bluuebunny/crossref_metadata_2025 huggingface dataset:
In Silico Genome-Wide Analysis of Respiratory Burst Oxidase Homolog (RBOH) Family Genes in Five Fruit-Producing Trees, and Potential Functional Analysis on Lignification of Stone Cells in Chinese White Pear
Authors: Cheng Xi , Li Guohui , Manzoor Muhammad Aamir , Wang Han , Abdullah Muhammad , Su Xueqiang , Zhang Jingyun , Jiang Taoshan , Jin Qing , Cai Yongping , Lin Yi
Published: May 2019

The accumulation of lignin in fruit has a significant negative impact on the quality of fruit-producing trees, and in particular the lignin formation stimulates the development of stone cells in pear fruit. Reactive oxygen species (ROS) are essential for lignin polymerization. However, knowledge of the RBOH family, a key enzyme in ROS metabolism, remains unknown in most fruit trees. In this study, a total of 40 RBOHs were identified from five fruit-producing trees (Pyrus bretschneideri, Prunus persica, Citrus sinensis, Vitis vinifera, and Prunus mume), and 10 of these sequences came from Pyrus bretschneideri. Multiple sequence alignments revealed that all 10 PbRBOHs contained the NADPH_Ox domain and the six alpha-helical transmembrane domains (TM-I to TM-VI). Chromosome localization and interspecies phylogenetic tree analysis showed that 10 PbRBOHs irregularly distributed on 8 chromosomes and 3 PbRBOHs (PbRBOHA, PbRBOHB, and PbRBOHD) are closely related to known lignification-related RBOHs. Furthermore, hormone response pattern analysis showed that the transcription of PbRBOHs is regulated by SA, ABA and MeJA. Reverse transcription-quantitative real-time polymerase chain reaction (qRT-PCR) and transcriptome sequencing analysis showed that PbRBOHA, PbRBOHB, and PbRBOHD accumulated high transcript abundance in pear fruit, and the transcriptional trends of PbRBOHA and PbRBOHD was consistent with the change of stone cell content during fruit development. In addition, subcellular localization revealed that PbRBOHA and PbRBOHD are distributed on the plasma membrane. Combining the changes of apoplastic superoxide (O2.−) content and spatio-temporal expression analysis, these results indicate that PbRBOHA and PbRBOHD, which are candidate genes, may play an important role in ROS metabolism during the lignification of pear stone cells. This study not only provided insight into the molecular characteristics of the RBOH family in fruit-producing trees, but also lays the foundation for studying the role of ROS in plant lignification.
A New Approach to Calculate the Shielding Factor of Magnetic Shields Comprising Nonlinear Ferromagnetic Materials under Arbitrary Disturbances
Authors: Zhao Yiyang , Sun Zhiyin , Pan Donghua , Lin Shengxin , Jin Yinxi , Li Liyi
Published: May 2019

To enable the realization of ultra-low magnetic fields for scientific and technological research, magnetic shielding is required to create a space with low residual magnetic field and high shielding factors. The shielding factors of magnetic shields are due to nonlinear material properties, the geometry and structure of the shields, and the external magnetic fields. Magnetic shielding is used in environments full of random realistic disturbances, resulting in an arbitrary and random external magnetic field, and in this case, the shielding effect is hard to define simply by the shielding factors. A new method to simulate and predict a dynamic internal space magnetic field wave is proposed based on the Finite Element method (FEM) combined with the Jiles-Atherton (JA) model. By simulating the hysteresis behavior of the magnetic shields and establishing a dynamic model, the new method can simulate dynamic magnetic field changes inside magnetic shields as long as the external disturbances are known. The shielding factors under an AC external field with a sine wave and certain frequencies are calculated to validate the feasibility of the new method. A real-time wave of internal magnetic flux density under an AC triangular wave external field is simulated directly with the new method versus a method that splits the triangular wave into several sine waves by a Fourier transform, divides the shielding factors, and then adds the quotients together. Moreover, real-time internal waves under some arbitrary fields are measured. Experimental internal magnetic flux density waves of a 4-layer magnetically shielded room (MSR) at the Harbin Institute of Technology (HIT) fit the simulated results well, taking experimental errors into account.
Effect of Alternative Liquid Fuels on the Exhaust Particle Size Distributions of a Medium-Speed Diesel Engine
Authors: Ovaska Teemu , Niemi Seppo , Sirviö Katriina , Heikkilä Sonja , Portin Kaj , Asplund Tomas
Published: May 2019

We mainly aimed to determine how alternative liquid fuels affect the exhaust particle size distributions (PSD) emitted by a medium-speed diesel engine. The selected alternative fuels included: circulation-origin marine gas oil (MGO), the 26/74 vol. % blend of renewable naphtha and baseline low-sulfur marine light fuel oil (LFO), and kerosene. PSDs were measured by means of an engine exhaust particle sizer from the raw exhaust of a four-cylinder, turbocharged, intercooled engine. During the measurements, the engine was loaded by an alternator, the maximum power output being set at 600 kW(e) at a speed of 1000 rpm. The partial loads of 450, 300, 150 and 60 kW(e) were also used for measurements. At each load, the PSDs had a distinct peak between 20 and 100 nm regardless of fuel. Relative to the other fuels, circulation-origin MGO emitted the lowest particle numbers at several loads despite having the highest viscosity and highest density. Compared to baseline LFO and kerosene, MGO and the blend of renewable naphtha and LFO were more beneficial in terms of total particle number (TPN). Irrespective of the load or fuel, the TPN consisted mainly of particles detected above the 23 nm size category.
Integrated Measuring and Control System for Thermal Analysis of Buildings Components in Hot Box Experiments
Authors: de Rubeis Tullio , Muttillo Mirco , Nardi Iole , Pantoli Leonardo , Stornelli Vincenzo , Ambrosini Dario
Published: May 2019

In this paper, a novel integrated measuring and control system for hot box experiments is presented. The system, based on a general-purpose microcontroller and on a wireless sensors network, is able to fully control the thermal phenomena inside the chambers, as well as the heat flux that involves the specimen wall. Thanks to the continuous measurements of air and surfaces temperatures and energy input into the hot chamber, the thermal behavior of each hot box component is analyzed. A specific algorithm allows the post-process of the measured data for evaluating the specimen wall thermal quantities and for creating 2D and 3D thermal models of each component. The system reliability is tested on a real case represented by a double insulating X-lam wall. The results of the 72 h experiment show the system’s capability to maintain stable temperature set points inside the chambers and to log the temperatures measured by the 135 probes, allowing to know both the U-value of the sample (equal to 0.216 ± 0.01 W/m2K) and the thermal models of all the hot box components. The U-value obtained via hot box method has been compared with the values gathered through theoretical calculation and heat flow meter measurements, showing differences of less than 20%. Finally, thanks to the data postprocessing, the 2D and 3D thermal models of the specimen wall and of the chambers have been recreated.
CFD Simulation and Experimental Study of Working Process of Screw Refrigeration Compressor with R134a
Authors: Wu Huagen , Huang Hao , Zhang Beiyu , Xiong Baoshun , Lin Kanlong
Published: May 2019

Twin-screw refrigeration compressors have been widely used in many industry applications due to their unique advantages. The performance of twin-screw refrigeration compressors is generally predicted by one-dimensional numerical simulation or empirical methods; however, the above methods cannot obtain the distribution of the fluid pressure field and temperature field inside the compressor. In this paper, a three-dimensional model was established based on the experimental twin-screw refrigeration compressor. The internal flow field of the twin-screw compressor was simulated by computational fluid dynamics (CFD) software using structured dynamic grid technology. The flow and thermodynamic characteristics of the fluid inside the compressor were analyzed. The distribution of the internal pressure field, temperature field, and velocity field in the compressor were obtained. Comparing the P-θ indicator diagram and the performance parameters of the compressor with the experimental results, it was found that the results of the three-dimensional numerical simulation were consistent with the experimental data. The maximum error was up to 2.578% on the adiabatic efficiency at the partial load working condition. The accuracy of the 3D numerical simulation of the screw compressors was validated and a new method for predicting the performance of twin-screw refrigeration compressors was presented that will be helpful in their design.
Development and 24 Hour Behavior Analysis of a Peak-Shaving Equipment with Battery Storage
Authors: Sant’Ana Wilson Cesar , Gonzatti Robson Bauwelz , Lambert-Torres Germano , Bonaldi Erik Leandro , Torres Bruno Silva , de Oliveira Pedro Andrade , Pereira Rondineli Rodrigues , Borges-da-Silva Luiz Eduardo , Mollica Denis , Santana Filho Joselino
Published: May 2019

This paper presents the development of a peak-shaving equipment, composed by a multilevel converter in a cascaded H-bridge topology and battery banks on the DC links. Between specific time periods, when the demand is higher, the equipment injects active power from the batteries into the grid to provide support to the system. During the other times of the day, when the demand is lower, the converter charges its battery banks with the exceeding (and low producing cost) energy from the grid. The charge and discharge control algorithms are implemented in a digital signal processor (DSP). The precise time of the day information is obtained from a real-time-clock from a global positioning system module (GPS), which communicates with the DSP through the serial interface. This paper presents the control algorithms and experimental results obtained in a 24 h continuous operation of the equipment.
Analytical and Experimental Investigation of the Solar Chimney System
Authors: Lipnicki Zygmunt , Gortych Marta , Staszczuk Anna , Kuczyński Tadeusz , Grabas Piotr
Published: May 2019

In this, paper the authors propose a new simplified method of solving the problem of air flow through a solar chimney system using a classical system of equations for the principles of conservation (momentum, mass, and energy), as well as a general solution to research the problem using similarity theory. The method presented in this paper allows one to design a solar chimney. The theoretical analysis was compared with experimental studies on existing solar towers. The experimental and theoretical studies were satisfactorily consistent. For clarity, the phenomenon of heat flow in the solar chimney was described using dimensionless numbers, such as the Reynolds, Grashof, Galileo, Biot, and Prandtl numbers. In the equations for the dimensionless geometric parameters, the ratios of the collector radius to the thickness gap, height, and chimney radius were used. The method used to test the system of equations allows us to analyse various solar collectors easily. In the scientific literature, there is a lack of a simple calculation method to use in engineering practice, suitable for each type of solar chimney independent of dimensions and construction parameters.
The Polyubiquitin Gene MrUBI4 Is Required for Conidiation, Conidial Germination, and Stress Tolerance in the Filamentous Fungus Metarhizium robertsii
Authors: Wang Zhangxun , Zhu Hong , Cheng Yuran , Jiang Yuanyuan , Li Yuandong , Huang Bo
Published: May 2019

The polyubiquitin gene is a highly conserved open reading frame that encodes different numbers of tandem ubiquitin repeats from different species, which play important roles in different biological processes. Metarhizium robertsii is a fungal entomopathogen that is widely applied in the biological control of pest insects. However, it is unclear whether the polyubiquitin gene is required for fungal development, stress tolerance, and virulence in the entomopathogenic fungus. In the present study, the polyubiquitin gene (MrUBI4, MAA_02160) was functionally characterized via gene deletion in M. robertsii. Compared to the control strains, the MrUBI4 deletion mutant showed delayed conidial germination and significantly decreased conidial yields (39% of the wild-type 14 days post-incubation). Correspondingly, the transcript levels of several genes from the central regulatory pathways associated with conidiation, including brlA, abaA, and wetA, were significantly downregulated, which indicated that MrUBI4 played an important role in asexual sporulation. Deletion of MrUBI4 especially resulted in increased sensitivity to ultraviolet (UV) and heat-shock stress based on conidial germination analysis between mutant and control strains. The significant increase in sensitivity to heat-shock was accompanied with reduced transcript levels of genes related to heat-shock protein (hsp), trehalose, and mannitol accumulation (tps, tpp, nth, and mpd) in the MrUBI4 deletion mutant. Deletion of MrUBI4 has no effect on fungal virulence. Altogether, MrUBI4 is involved in the regulation of conidiation, conidial germination, UV stress, and heat-shock response in M. robertsii.
Impact of the Case Management Model through Community Liaison Nurses
Authors: Duarte-Climents Gonzalo , Sánchez-Gómez María Begoña , Rodríguez-Gómez José Ángel , Rodríguez-Álvarez Cristobalina , Sierra-López Antonio , Aguirre-Jaime Armando , Gómez-Salgado Juan
Published: May 2019

The objective of the present study is to assess the model’s impact on patients and their families in terms of outcomes and the efficiency results for the health system in Tenerife, Canary Islands, selecting a period of eight years from the time interval 2002–2018. The employed indicators were collected on a monthly basis. They referred to home care and its impact on clinical outcomes and on the use of resources. The comparison between the indicators’ tendencies with and without the liaison nurse model was done with the F-test by Snedecor. All these tests are bilateral, with a level of significance of p < 0.05. In those areas with community liaison nurse (CLN), improvements have been found in indicators that describe: (1) the management of the clinical status of patients, (2) the efficiency of the use of resources, and (3) the quality and compliance with the process that also includes home visits and social risk detection and management. It can be said that in the basic areas of primary health care where the work of the CLN develops there are improvements in the management of the patients’ clinical condition as well as in the quality and efficiency of care.
Understanding Global HIV Stigma and Discrimination: Are Contextual Factors Sufficiently Studied? (GAPRESEARCH)
Authors: Tran Bach Xuan , Phan Hai Thanh , Latkin Carl A. , Nguyen Huong Lan Thi , Hoang Chi Linh , Ho Cyrus S.H. , Ho Roger C.M.
Published: May 2019

Stigma and discrimination are among the greatest challenges that people living with human immunodeficiency virus (HIV) face, and both are known to negatively affect quality of life as well as treatment outcomes. We analyzed the growing research and current understanding of HIV-related stigma and contextual factors in HIV/AIDS (human Immunodeficiency virus/ acquired immunodeficiency syndrome) bibliography. A total of 5984 publications published from 1991 to 2017 were retrieved from the Web of Science database. The number of papers and their impacts have been considerably grown in recent years. Research landscapes related to stigma and discrimination include clinical, physical and mental health outcomes, risk behaviors of most-at-risk populations, and HIV-related services. We found a lack of empirical studies not only on social, cultural and economic contexts, but also on specific interventions for particular settings and sub-populations. This study highlights certain gaps and provides a basis for future studies and interventions on this critical issue given the changing drivers of HIV epidemics.
The Role of Socioeconomic Status in the Association of Lung Function and Air Pollution—A Pooled Analysis of Three Adult ESCAPE Cohorts
Authors: Keidel Dirk , Anto Josep Maria , Basagaña Xavier , Bono Roberto , Burte Emilie , Carsin Anne-Elie , Forsberg Bertil , Fuertes Elaine , Galobardes Bruna , Heinrich Joachim , de Hoogh Kees , Jarvis Debbie , Künzli Nino , Leynaert Bénédicte , Marcon Alessandro , Le Moual Nicole , de Nazelle Audrey , Schindler Christian , Siroux Valérie , Stempfelet Morgane , Sunyer Jordi , Temam Sofia , Tsai Ming-Yi , Varraso Raphaëlle , Jacquemin Bénédicte , Probst-Hensch Nicole
Published: May 2019

Ambient air pollution is a leading environmental risk factor and its broad spectrum of adverse health effects includes a decrease in lung function. Socioeconomic status (SES) is known to be associated with both air pollution exposure and respiratory function. This study assesses the role of SES either as confounder or effect modifier of the association between ambient air pollution and lung function. Cross-sectional data from three European multicenter adult cohorts were pooled to assess factors associated with lung function, including annual means of home outdoor NO2. Pre-bronchodilator lung function was measured according to the ATS-criteria. Multiple mixed linear models with random intercepts for study areas were used. Three different factors (education, occupation and neighborhood unemployment rate) were considered to represent SES. NO2 exposure was negatively associated with lung function. Occupation and neighborhood unemployment rates were not associated with lung function. However, the inclusion of the SES-variable education improved the models and the air pollution-lung function associations got slightly stronger. NO2 associations with lung function were not substantially modified by SES-variables. In this multicenter European study we could show that SES plays a role as a confounder in the association of ambient NO2 exposure with lung function.
Does CABG with Saphenous Vein Grafting and Standard Cardiac Rehabilitation Affect Lower Limb Function? A Clinical Study
Authors: Skomudek Aleksandra , Waz Grzegorz , Rozek-Piechura Krystyna
Published: May 2019

Background: The aim of the study was to assess lower limb function in response to two cardiac rehabilitation (CR) protocols after coronary artery bypass surgery with saphenous vein grafting. Methods: Clinically-stable male patients aged 50–70 years were recruited 4 weeks post-surgery in which to group. Group I (n = 47) receive standard CR in a hospital setting for 3 weeks and Group II (n = 14) receive CR with a resistance training component in an outpatient setting for 8 weeks. Measures included body mass and composition, lower limb temperature distribution, lower limb hemodynamics, and dorsal and plantar flexor muscle strength. Results: Average temperature of the operated limb decreased only in Group II after cardiac rehabilitation. Venous blood flow improved in both groups as evidenced by increased blood refilling time. Isokinetic strength was greater in Group I. Conclusions: The results suggest a 3-week intensive CR protocol to be most effective in restoring lower limb function in CABG patients after saphenectomy.
Particulate Matter Mortality Rates and Their Modification by Spatial Synoptic Classification
Authors: Kim Jayeun
Published: May 2019

Air pollution levels are highly correlated with temperature or humidity, so we investigated the relationship between PM10 and the spatial synoptic classification (SSC) scheme on daily mortality, according to age group and season. Daily death data for 2000–2014 from Seoul, Korea, were acquired, and time-series analysis was applied with respect to season and to each of seven distinct SSC types: dry moderate (DM); dry polar (DP); dry tropical (DT); moist moderate (MM); moist polar (MP); moist tropical (MT); and transition (T). Modification effects were estimated for daily, non-accidental, cardiovascular, and respiratory mortality between PM10 and SSC types. The following SSC-type-specific increased mortalities were observed, by cause of death: non-accidental mortality: DT (1.86%) and MT (1.86%); cardiovascular mortality: DT (2.83%) and MM (3.00%); respiratory mortality: MT (3.78%). Based on simplified weather types, increased PM10 effects in non-accidental mortality rates were observed in dry (1.54%) and moist (2.32%) conditions among those aged 40–59 years and were detected regardless of conditions in other age groups: 60–74 (1.11%), 75–84 (1.55%), and 85+ (1.75%). The effects of particulate air pollution, by SSC, suggest the applicability of SSC to the comparison and understanding of acute effects of daily mortality based on weather type.
Galectin-9 Induces Mitochondria-Mediated Apoptosis of Esophageal Cancer In Vitro and In Vivo in a Xenograft Mouse Model
Authors: Chiyo Taiga , Fujita Koji , Iwama Hisakazu , Fujihara Shintaro , Tadokoro Tomoko , Ohura Kyoko , Matsui Takanori , Goda Yasuhiro , Kobayashi Nobuya , Nishiyama Noriko , Yachida Tatsuo , Morishita Asahiro , Kobara Hideki , Mori Hirohito , Niki Toshiro , Hirashima Mitsuomi , Himoto Takashi , Masaki Tsutomu
Published: May 2019

Galectin-9 (Gal-9) enhances tumor immunity mediated by T cells, macrophages, and dendritic cells. Its expression level in various cancers correlates with prognosis. Furthermore, Gal-9 directly induces apoptosis in various cancers; however, its mechanism of action and bioactivity has not been clarified. We evaluated Gal-9 antitumor effect against esophageal squamous cell carcinoma (ESCC) to analyze the dynamics of apoptosis-related molecules, elucidate its mechanism of action, and identify relevant changes in miRNA expressions. KYSE-150 and KYSE-180 cells were treated with Gal-9 and their proliferation was evaluated. Gal-9 inhibited cell proliferation in a concentration-dependent manner. The xenograft mouse model established with KYSE-150 cells was administered with Gal-9 and significant suppression in the tumor growth observed. Gal-9 treatment of KYSE-150 cells increased the number of Annexin V-positive cells, activation of caspase-3, and collapse of mitochondrial potential, indicating apoptosis induction. c-Jun NH2-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38) phosphorylation were activated and could be involved in apoptosis. Therefore, Gal-9 induces mitochondria-mediated apoptosis of ESCC and inhibits cell proliferation in vitro and in vivo with JNK and p38 activation.
Multi-Walled Carbon Nanotube-Induced Gene Expression Biomarkers for Medical and Occupational Surveillance
Authors: Snyder-Talkington Brandi N. , Dong Chunlin , Singh Salvi , Raese Rebecca , Qian Yong , Porter Dale W. , Wolfarth Michael G. , Guo Nancy L.
Published: May 2019

As the demand for multi-walled carbon nanotube (MWCNT) incorporation into industrial and biomedical applications increases, so does the potential for unintentional pulmonary MWCNT exposure, particularly among workers during manufacturing. Pulmonary exposure to MWCNTs raises the potential for development of lung inflammation, fibrosis, and cancer among those exposed; however, there are currently no effective biomarkers for detecting lung fibrosis or predicting the risk of lung cancer resulting from MWCNT exposure. To uncover potential mRNAs and miRNAs that could be used as markers of exposure, this study compared in vivo mRNA and miRNA expression in lung tissue and blood of mice exposed to MWCNTs with in vitro mRNA and miRNA expression from a co-culture model of human lung epithelial and microvascular cells, a system previously shown to have a higher overall genome-scale correlation with mRNA expression in mouse lungs than either cell type grown separately. Concordant mRNAs and miRNAs identified by this study could be used to drive future studies confirming human biomarkers of MWCNT exposure. These potential biomarkers could be used to assess overall worker health and predict the occurrence of MWCNT-induced diseases.
Is Chickpea a Potential Substitute for Soybean? Phenolic Bioactives and Potential Health Benefits
Authors: de Camargo Adriano Costa , Favero Bruno Trevenzoli , Morzelle Maressa Caldeira , Franchin Marcelo , Alvarez-Parrilla Emilio , de la Rosa Laura A. , Geraldi Marina Vilar , Maróstica Júnior Mário Roberto , Shahidi Fereidoon , Schwember Andrés R.
Published: May 2019

Legume seeds are rich sources of protein, fiber, and minerals. In addition, their phenolic compounds as secondary metabolites render health benefits beyond basic nutrition. Lowering apolipoprotein B secretion from HepG2 cells and decreasing the level of low-density lipoprotein (LDL)-cholesterol oxidation are mechanisms related to the prevention of cardiovascular diseases (CVD). Likewise, low-level chronic inflammation and related disorders of the immune system are clinical predictors of cardiovascular pathology. Furthermore, DNA-damage signaling and repair are crucial pathways to the etiology of human cancers. Along CVD and cancer, the prevalence of obesity and diabetes is constantly increasing. Screening the ability of polyphenols in inactivating digestive enzymes is a good option in pre-clinical studies. In addition, in vivo studies support the role of polyphenols in the prevention and/or management of diabetes and obesity. Soybean, a well-recognized source of phenolic isoflavones, exerts health benefits by decreasing oxidative stress and inflammation related to the above-mentioned chronic ailments. Similar to soybeans, chickpeas are good sources of nutrients and phenolic compounds, especially isoflavones. This review summarizes the potential of chickpea as a substitute for soybean in terms of health beneficial outcomes. Therefore, this contribution may guide the industry in manufacturing functional foods and/or ingredients by using an undervalued feedstock.
Formation of γH2AX and pATM Foci in Human Mesenchymal Stem Cells Exposed to Low Dose-Rate Gamma-Radiation
Authors: Ulyanenko Stepan , Pustovalova Margarita , Koryakin Sergey , Beketov Evgenii , Lychagin Anatolii , Ulyanenko Liliya , Kaprin Andrey , Grekhova Anna , M. Ozerova Alexandra , V. Ozerov Ivan , Vorobyeva Natalia , Shegay Peter , Ivanov Sergey , Leonov Sergey , Klokov Dmitry , Osipov Andreyan N.
Published: May 2019

DNA double-strand breaks (DSB) are among the most harmful DNA lesions induced by ionizing radiation (IR). Although the induction and repair of radiation-induced DSB is well studied for acute irradiation, responses to DSB produced by chronic IR exposures are poorly understood, especially in human stem cells. The aim of this study was to examine the formation of DSB markers (γH2AX and phosphorylated kinase ATM, pATM, foci) in human mesenchymal stem cells (MSCs) exposed to chronic gamma-radiation (0.1 mGy/min) in comparison with acute irradiation (30 mGy/min) at cumulative doses of 30, 100, 160, 240 and 300 mGy. A linear dose-dependent increase in the number of both γH2AX and pATM foci, as well as co-localized γH2AX/pATM foci (“true” DSB), were observed after an acute radiation exposure. In contrast, the response of MSCs to a chronic low dose-rate IR exposure deviated from linearity towards a threshold model, for γH2AX, pATM foci and γH2AX/pATM foci, with an indication of a “plateau”. The state of equilibrium between newly formed DSB at a low rate during the protracted exposure time and the elimination of a fraction of DSB is proposed as a mechanistic explanation of the non-linear DSB responses following a low dose-rate irradiation. This notion is supported by the observation of the elimination of a substantial fraction of DSB 6 h after the cessation of the exposures. Our results demonstrate non-linear dose responses for γH2AX and pATM foci in human MSCs exposed to low dose-rate IR and showed the existence of a threshold, which may have implications for radiation protection in humans.
AMPK Alters Detrusor Contractility During Emptying in Normal Bladder and Hypertrophied Bladder with Partial Bladder Outlet Obstruction via CaMKKβ
Authors: Choi Bo-Hwa , Jin Long-Hu , Chung Doo Yong , Cho Tae Jin , Kang Ju-Hee , Lee Tack , Park Chang-Shin
Published: May 2019

AMP-activated protein kinase (AMPK) has been implicated in contractility changes in bladders with partial bladder outlet obstruction (PBOO), but the role of AMPK in the contractile response of normal bladder remains unclear. We investigated the phosphorylation of AMPKα and expression of the involved upstream AMPK kinases (AMPKKs) in a model of bladders with PBOO and sought to determine whether the pharmacological inhibition of these two factors affected detrusor contractility in normal bladders, using female Sprague–Dawley rats. Cystometry and Western blot analysis were performed in rats that were subjected to PBOO induction or a sham operation. Cystometry was performed in normal rats that received selective inhibitors of AMPKα and Ca2+/calmodulin-dependent protein kinase kinase (CaMKKβ) (compound C and STO-609, respectively) at doses determined in the experiments. In the PBOO bladders, bladder weight and micturition pressure (MP) were higher and AMPKα phosphorylation (T172) and CaMKKβ expression was significantly reduced. Compound C and STO-609 increased MP. The increased contractile response in bladders with PBOO-induced hypertrophy was related to decreased CaMKKβ/AMPK signaling activity, and the pharmacological inhibition of this pathway in normal bladders increased detrusor contractility, implying a role of CaMKKβ/AMPK signaling in the bladder in the regulation of detrusor contractility.
Potential for Acanthoscelides obtectus to Adapt to New Hosts Seen in Laboratory Selection Experiments
Authors: Savković Uroš , Đorđević Mirko , Stojković Biljana
Published: May 2019

Effective pest management strategies for a targeted pest species must rely on accurate, reliable and reproducible estimates of population dynamics. Importance of such approaches is even more conspicuous when assessing pest’s potential to utilize other stored products. Using an experimental evolution approach, we have focused our attention on a common bean pest, the seed beetle (Acanthoscelides obtectus). We looked into the potential to invade and sustain population growth on two suboptimal host plants (chickpeas and mung beans). Such an approach simulates steps of the host-shift process in storages. By analyzing population dynamics during initial encountering with a new host plant, we detected a population drop for both novel hosts. However, transgenerational development in a novel environment resulted in a constant population growth in chickpeas, but not in mung bean populations. Reversal of chickpea selected populations to original host plant has led to a severe decrease in population parameters due to low viability of immatures, while the opposite trend was detected in mung bean populations. This paper highlights the importance of good practice in estimating population dynamics for economically important species. With special emphasis on storage pest species, we discuss how this approach can be useful for estimating invading potential of pest insects.
RGD-Modified Nanofibers Enhance Outcomes in Rats after Sciatic Nerve Injury
Authors: Cavanaugh McKay , Silantyeva Elena , Pylypiv Koh Galina , Malekzadeh Elham , Lanzinger William D. , Willits Rebecca Kuntz , Becker Matthew L.
Published: May 2019

Nerve injuries requiring surgery are a significant problem without good clinical alternatives to the autograft. Tissue engineering strategies are critically needed to provide an alternative. In this study, we utilized aligned nanofibers that were click-modified with the bioactive peptide RGD for rat sciatic nerve repair. Empty conduits or conduits filled with either non-functionalized aligned nanofibers or RGD-functionalized aligned nanofibers were used to repair a 13 mm gap in the rat sciatic nerve of animals for six weeks. The aligned nanofibers encouraged cell infiltration and nerve repair as shown by histological analysis. RGD-functionalized nanofibers reduced muscle atrophy. During the six weeks of recovery, the animals were subjected to motor and sensory tests. Sensory recovery was improved in the RGD-functionalized nanofiber group by week 4, while other groups needed six weeks to show improvement after injury. Thus, the use of functionalized nanofibers provides cues that aid in in vivo nerve repair and should be considered as a future repair strategy.
(2S,5R)-2-Isopropyl-5-methylcyclohexanone Hydrazones
Authors: Nesterkina Mariia , Barbalat Dmytro , Zheltvay Ivan , Rakipov Ildar , Atakay Mehmet , Salih Bekir , Kravchenko Iryna
Published: May 2019

Hydrazones were obtained in 76–78% yield via condensation of (2S,5R)-2-isopropyl-5-methylcyclohexanone with 4-R-phenoxyacetic acid hydrazides in the presence of a catalytic amount of glacial acetic acid. The structure of the target compounds has been established by FTIR-ATR, Raman, 1H-NMR and 13C-NMR spectral analysis and EI/FAB/ESI mass spectrometry. Thermal properties of hydrazones 3a–3e were elucidated by differential scanning calorimetry (DSC) and their purity by HPLC coupled to mass spectrometry. Synthesized compounds were found to exist as Z/E geometrical isomers about C=N bond and cis/trans amide conformers.
Modeling the Physical Properties of Gamma Alumina Catalyst Carrier Based on an Artificial Neural Network
Authors: Majdi Hasan Sh. , Saud Amir N. , Saud Safaa N.
Published: May 2019

Porous γ-alumina is widely used as a catalyst carrier due to its chemical properties. These properties are strongly correlated with the physical properties of the material, such as porosity, density, shrinkage, and surface area. This study presents a technique that is less time consuming than other techniques to predict the values of the above-mentioned physical properties of porous γ-alumina via an artificial neural network (ANN) numerical model. The experimental data that was implemented was determined based on 30 samples that varied in terms of sintering temperature, yeast concentration, and socking time. Of the 30 experimental samples, 25 samples were used for training purposes, while the other five samples were used for the execution of the experimental procedure. The results showed that the prediction and experimental data were in good agreement, and it was concluded that the proposed model is proficient at providing high accuracy estimation data derived from any complex analytical equation.
In Vitro and In Vivo Neuroprotective Effects of Stellettin B Through Anti-Apoptosis and the Nrf2/HO-1 Pathway
Authors: Feng Chien-Wei , Chen Nan-Fu , Wen Zhi-Hong , Yang Wen-Ya , Kuo Hsiao-Mei , Sung Ping-Jyun , Su Jui-Hsin , Cheng Shu-Yu , Chen Wu-Fu
Published: May 2019

Pharmaceutical agents for halting the progression of Parkinson’s disease (PD) are lacking. The current available medications only relieve clinical symptoms and may cause severe side effects. Therefore, there is an urgent need for novel drug candidates for PD. In this study, we demonstrated the neuroprotective activity of stellettin B (SB), a compound isolated from marine sponges. We showed that SB could significantly protect SH-SY5Y cells against 6-OHDA-induced cellular damage by inhibiting cell apoptosis and oxidative stress through PI3K/Akt, MAPK, caspase cascade modulation and Nrf2/HO-1 cascade modulation, respectively. In addition, an in vivo study showed that SB reversed 6-OHDA-induced a locomotor deficit in a zebrafish model of PD. The potential for developing SB as a candidate drug for PD treatment is discussed.
Crohn’s Disease: Potential Drugs for Modulation of Autophagy
Authors: Azzman Nursyuhada
Published: May 2019

Autophagy is an intracellular process whereby cytoplasmic constituents are degraded within lysosomes. Autophagy functions to eliminate unwanted or damaged materials such as proteins and organelles as their accumulation would be harmful to the cellular system. Autophagy also acts as a defense mechanism against invading pathogens and plays an important role in innate and adaptive immunity. In physiological processes, autophagy is involved in the regulation of tissue development, differentiation and remodeling, which are essential for maintaining cellular homeostasis. Recent studies have demonstrated that autophagy is linked to various diseases and involved in pathophysiological roles, such as adaptation during starvation, anti-aging, antigen presentation, tumor suppression and cell death. The modulation of autophagy has shown greatest promise in Crohn’s disease as most of autophagy drugs involved in these diseases are currently under clinical trials and some has been approved by Food and Drug Administration. This review article discusses autophagy and potential drugs that are currently available for its modulation in Crohn’s disease.
Femtosecond Laser Micromachining of Soda–Lime Glass in Ambient Air and under Various Aqueous Solutions
Authors: Mačernytė Lina , Skruibis Julius , Vaičaitis Virgilijus , Sirutkaitis Romualdas , Balachninaitė Ona
Published: May 2019

We have studied femtosecond ablation of soda–lime glass sample under thin water film, under KOH and NaCl aqueous solutions films and their influence and benefits compared with ablation in the air atmosphere. These have been studied in case of the groove ablation using the infrared (IR) femtosecond laser. KOH aqueous solution film above the glass sample improved the ablation efficiency and led to the formation of the grooves with a higher aspect ratio when multi-scan glass cutting conditions were applied.
Coupling Mineralogy, Textures, Stable and Radiogenic Isotopes in Identifying Ore-Forming Processes in Irish-Type Carbonate-Hosted Zn–Pb Deposits
Authors: Yesares Lola , Drummond Drew A. , Hollis Steven P. , Doran Aileen L. , Menuge Julian F. , Boyce Adrian J. , Blakeman Robert J. , Ashton John H.
Published: May 2019

Irish-type deposits comprise carbonate-hosted sphalerite- and galena-rich lenses concentrated near normal faults. We present new data from the Tara Deep resource and overlying mineralization, at Navan, and the Island Pod deposit and associated Main zone orebodies, at Lisheen. Tara Deep mineralization predominantly replaces Tournasian micrites and subordinate Visean sedimentary breccias. The mineralization is mainly composed of sphalerite, galena, marcasite and pyrite. A range of Cu- and Sb-bearing minerals occur as minor phases. At Tara Deep, paragenetically early sulfides exhibit negative δ34S values, with later phases displaying positive δ34S values, indicating both bacterial sulfate reduction (BSR) and hydrothermal sulfur sources, respectively. However, maximum δ34S values are heavier (25‰) than in the Main Navan orebody (17‰). These mineralogical and isotopic features suggest that Tara Deep represents near-feeder mineralization relative to the Navan Main orebody. The subeconomic mineralization hosted in the overlying Thin Bedded Unit (TBU) comprises sphalerite replacing framboidal pyrite, both exhibiting negative δ34S values (−37.4 to −8.3‰). These features indicate a BSR source of sulfur for TBU mineralization, which may represent seafloor exhalation of mineralizing fluids that formed the Tara Deep orebody. The Island Pod orebody, at Lisheen, shows a mineralogical paragenetic sequence and δ34S values broadly similar to other Lisheen orebodies. However, the lack of minor Cu, Ni, and Sb minerals suggests a setting more distal to hydrothermal metal feeder zones than the other Lisheen orebodies. Pb isotope data indicate a very homogeneous Lower Palaeozoic Pb source for all Navan orebodies. Lower Palaeozoic metal sources are also inferred for Lisheen, but with variations both within and between orebodies. Carbon and oxygen isotopic variations at Navan and Lisheen appear to result from fluid-carbonate rock buffering. The emerging spectrum of mineralogical and isotopic variations define proximal to distal characteristics of Irish-type systems and will assist in developing geochemical vectoring tools for exploration.
Phenolic Compounds from Five Ericaceae Species Leaves and Their Related Bioavailability and Health Benefits
Authors: Ștefănescu Bianca Eugenia , Szabo Katalin , Mocan Andrei , Crişan Gianina
Published: May 2019

Some species of the Ericaceae family have been intensively studied because of the beneficial health impact, known since ancient times, of their chemical components. Since most studies focus on the effects of fruit consumption, this review aims to highlight the phenolic components present in the leaves. For this purpose, five species from Ericaceae family (bilberry—Vaccinium myrtillus L., lingonberry—V. vitis-idaea L., bog bilberry—V. uliginosum L., blueberry—V. corymbosum L. and bearberry—Arctostapylos uva-ursi L.) were considered, four of which can be found in spontaneous flora. The chemical composition of the leaves revealed three major phenolic compounds: chlorogenic acid, quercetin and arbutin. The health promoting functions of these compounds, such as antioxidant and anti-inflammatory properties that could have preventive effects for cardiovascular disease, neurodegenerative disorders, cancer, and obesity, have been exemplified by both in vitro and in vivo studies in this review. Furthermore, the importance of bioaccessibility and bioavailability of the phenolic compounds have been summarized. The findings highlight the fact that leaves of some Ericaceae species deserve increased attention and should be studied more profoundly for their biological activities, especially those from spontaneous flora.
A Malonyl-Based Scaffold for Conjugatable Multivalent Carbohydrate-BODIPY Presentations
Authors: Uriel Clara , Sola-Llano Rebeca , Bañuelos Jorge , Gomez Ana , Lopez J.
Published: May 2019

A concise synthetic route from methylmalonate to a tetravalent aliphatic scaffold has been developed. The ensuing tetra-tethered derivative is equipped with two hydroxyl groups, as well as orthogonal alkene and alkyne functionalities. The usefulness of the scaffold has been demonstrated with the preparation of two representative multivalent derivatives: (i) a tetravalent compound containing two D-mannose units, one fluorescent boron-dipyrromethene (BODIPY) dye and a suitably functionalized amino acid and (ii) by way of dimerization and saponification, a water-soluble tetramannan derivative containing two fluorescent BODIPY units. Additionally, photophysical measurements conducted on these derivatives support the viability of the herein designed single and double BODIPY-labeled carbohydrate-based clusters as fluorescent markers.
Conductivity and Density of States of New Polyphenylquinoline
Authors: Saitov Shamil R. , Amasev Dmitriy V. , Tameev Alexey R. , Malov Vladimir V. , Tedoradze Marine G. , Svetlichnyi Valentin M. , Myagkova Lyudmila A. , Popova Elena N. , Kazanskii Andrey G.
Published: May 2019

Electrical, photoelectrical, and optical properties of thin films of a new heat-resistant polyphenylquinoline synthesized using facile methods were investigated. An analysis of the obtained temperature dependences of the dark conductivity and photoconductivity indicates the hopping mechanism of conductivity over localized states arranging at the energy distance of 0.8 eV from the Fermi level located inside the band gap of the investigated material. The optical band gap of the studied material was estimated from an analysis of the spectral dependences of the photoconductivity and absorption coefficient before (1.8–1.9 eV) and after (2.0–2.2 eV) annealing at temperatures exceeding 100 °C. The Gaussian character of the distribution of the localized states of density inside the band gap near the edges of the bands was established. A mechanism of changes in the optical band gap of the investigating polymer under its annealing is proposed.
Why the Covenant Worked: On the Institutional Foundations of the American Civil Religion
Authors: Compton John W.
Published: May 2019

Scholars of American civil religion (ACR) have paid insufficient attention to the micro-level processes through which civil religious ideas have historically influenced beliefs and behavior. We know little about what makes such appeals meaningful to average Americans (assuming they are meaningful); nor do we know much about the mechanisms through which abstract religious themes and imagery come to be associated with specific policy aims, or what Robert Bellah called “national goals.” This article argues that a renewed focus on the relationship between civil religion and organized religion can help fill this gap in the literature. More specifically, I draw attention to three mainline Protestant institutions that for much of the twentieth-century were instrumental both in cultivating respect for the national civic faith and in connecting its abstract ideals to concrete reform programs: namely, the clergy, the state and local church councils, and the policy-oriented departments of the National Council of Churches (NCC). Finally, I argue that a fresh look at the relationship between civil religion and “church religion” sheds new light on the (arguably) diminished role of civil religious appeals in the present. If, as Bellah claimed in his later writings, ACR appeals have lost much of their power to motivate support for shared national goals, it is at least in part because the formal religious networks through which they once were transmitted and interpreted have largely collapsed.
As-Built 3D Heritage City Modelling to Support Numerical Structural Analysis: Application to the Assessment of an Archaeological Remain
Authors: Antón Daniel , Pineda Paloma , Medjdoub Benachir , Iranzo Alfredo
Published: May 2019

Terrestrial laser scanning is a widely used technology to digitise archaeological, architectural and cultural heritage. This allows for modelling the assets’ real condition in comparison with traditional data acquisition methods. This paper, based on the case study of the basilica in the Baelo Claudia archaeological ensemble (Tarifa, Spain), justifies the need of accurate heritage modelling against excessively simplified approaches in order to support structural safety analysis. To do this, after validating the 3D meshing process from point cloud data, the semi-automatic digital reconstitution of the basilica columns is performed. Next, a geometric analysis is conducted to calculate the structural alterations of the columns. In order to determine the structural performance, focusing both on the accuracy and suitability of the geometric models, static and modal analyses are carried out by means of the finite element method (FEM) on three different models for the most unfavourable column in terms of structural damage: (1) as-built (2) simplified and (3) ideal model without deformations. Finally, the outcomes show that the as-built modelling enhances the conservation status analysis of the 3D heritage city (in terms of realistic compliance factor values), although further automation still needs to be implemented in the modelling process.