bluuebunny/crossref_metadata_2025 huggingface dataset:
Peran Guru Pendidikan Agama Islam Dalam Menanamkan Pendidikan Multikultural Berbasis Nilai-Nilai Kearifan Lokal
Authors: Supriyanto  , Amrin
Published: March 2023

This study aims to determine the role of Islamic Religious Education teachers in instilling multicultural education based on local wisdom values ​​in Indonesia State Junior High School 15 Surakarta. Method The research used is a descriptive qualitative method with data sources obtained from the principal, teachers, and students. The data analysis used is data collection, data reduction, data presentation, and concluding. Data collection techniques are observation, interviews, and documentation as for the analysis technique through the reduction stage, data presentation stage, and stage conclusion. The results of the study indicate that the role of Islamic Religious Education teachers in instilling multicultural education based on local wisdom values ​​in Indonesia State Junior High School 15 Surakarta  is teachers as educators,  motivators, facilitators, with multicultural education based on local wisdom in the form of values ​​of equality, tolerance, democracy, pluralism and human values ​​(morals). Planting multicultural education based on local wisdom values is also influenced by supporting factors, namely first, support from all parties, and secondly, the school facilities are adequate. While the inhibiting factor namely the lack of good cooperation between the school and parents and guardians in shaping the character and personality of noble character in children or students.
Pengaruh Kemampuan Teknologi Digital Terhadap Prestasi Siswa SMA di JABODETABEK
Authors: Wardaya Anton , Budi Kurniawan Novianto , Hadumaon Siagian Tiodora
Published: March 2023

Penelitian ini bertujuan untuk mengetahui pengaruh kemampuan teknologi digital terhadap prestasi siswa SMA di jabodetabek. Penelitian ini menggunakan jenis penelitian kuantitatif. Populasi  dalam penelitian ini yaitu siswa SMA di jabodetabek. Teknik pengambilan sampel dilakukan dengan teknik non probability sampling jenis sampling incidental. Selanjutnya dilakukan perhitungan menggunakan Rumus Slovin sehingga diperoleh sampel sebanyak 200 siswa. Teknik pengumpulan data yang digunakan yaitu kuesioner, dokumentasi dan wawancara. Data penelitian selanjutnya dianalisis menggunakan analisis regresi linear sederhana dengan bantuan SPSS 16.0. Hasil analisis data diperoleh bahwa nilai sig sebesar 0,00, maka tolak Ho sehingga dapat disimpulkan bahwa terdapat pengaruh kemampuan teknologi digital terhadap prestasi siswa. Besar pengaruh kemampuan teknologi terhadap prestasi siswa yaitu 51,80% dan sisanya 48,20% dipengaruhi oleh variabel lain.
Pengelolaan Kewirausahaan Sekolah Bagi Kepala Sekolah Dasar Negeri
Authors: Rosyidi Unifah , Karnati Neti , Rugaiyah Rugaiyah
Published: March 2023

Administrasi sekolah dasar merupakan kegiatan penunjang keberhasilan mutu pendidikan di sekolah dasar. Ruang lingkup kegiatan administrasi sekolah diantaranya adalah pengelolaan surat dinas dan arsip sekolah. Pengelolaan surat dinas dan arsip di sekolah dasar dilakukan oleh kepala sekolah dibantu oleh seorang tenaga operator sekolah. Kemampuan mereka dalam mengelola surat dinas dan arsip sekolah masih sangat kurang. Upaya peningkatan kemampuan para Kepala Sekolah dan tenaga administrasi sekolah dasar dapat diberikan pelatihan tentang pengelolaan surat dinas dan arsip sekolah. Objek (khalayak sasaran) kegiatan pelatihan sebanyak 25 Kepala SDN di kecamatan Pulo Gadung. Mitra yang terlibat adalah Suku Dinas Pendidikan Wilayah I Kota Administrasi Jakarta Timur. Pelatihan sudah dilaksanaka selama sehari pada hari Senin, 15 Agustus 2022 melalui virtual dengan zoom cloud metting. Materi pelatihan terdiri dari: kewirausahaan dan Pendidikan kewirausahaandi sekolah; peran kepala sekolah dalam kewirausahaan; dan pengelolaan kewirausahaan sekolah dasar.  Ketiga materi disampaikan oleh 3 orang dosen prodi Manajemen Pendidian FIP UNJ dengan metode ceramah, tanya jawab dan tugas. Hasil pelatihan sangat memuaskan peserta dan panitia pelaksana, karena semua materi telah disampaikan dengan baik dan sangat bermanfaat bagi semua peserta pelatihan.
Measuring with confidence: leveraging expressive type systems for correct-by-construction software
Authors: McBride Conor , Nakov Georgi , Nordvall Forsberg Fredrik
Published: March 2023

Modern programming language type systems help programmers write correct software, and furthermore helps them write the software they actually intended to write. We show how expressive types can be used to encode dimension and units of measure information, which can be used to avoid dimensional mistakes and guide software construction, and how types can even help to generate code automatically, which eliminates a whole class of bugs.
Uso problemático de Internet por parte de los menores desde la perspectiva parental antes y después del confinamiento general por COVID-19
Authors: Ponce-Gómez Juana , Zych Izabela , Rodríguez-Ruiz Joaquín
Published: March 2023

El uso problemático de Internet por parte de la población infantil y adolescente se ha incrementado en los últimos años, provocando consecuencias negativas en el desarrollo psicoemocional, físico, social y académico del menor. Sin embargo, pocos estudios han examinado el cambio en el uso problemático de Internet por parte de los menores tras la pandemia. Así, el objetivo principal de esta investigación fue estudiar si se ha producido un aumento en el uso problemático de Internet tras el confinamiento por COVID-19, analizar si este hipotético aumento se relaciona con la supervisión parental y explorar su vínculo con edad, género y actividades online no educativas. Este fue un estudio retrospectivo que incluyó a 156 padres y madres de menores (de entre 5 y 16 años), que respondieron a una batería de cuestionarios con buenas propiedades psicométricas para evaluar el uso problemático que realizan sus hijos de Internet, la frecuencia de uso de las actividades que los menores realizan en la red y la supervisión que ellos mismos otorgan a sus hijos. Estas variables se compararon entre antes y después del confinamiento por COVID-19. Los resultados mostraron que se ha producido un aumento del uso problemático de Internet por parte de los menores tras el confinamiento. Por otro lado, los hallazgos no mostraron una relación significativa entre la supervisión parental y un menor uso problemático de Internet en ninguno de los dos momentos temporales. Cabe destacar que la supervisión que ejercen los padres no ha sufrido cambios a pesar del aumento del uso problemático de Internet tras el confinamiento.
Decompositional processes of microbial carbonates in Lagoa Vermelha, Brazil
Authors: Shiraishi Fumito , Hanzawa Yusaku , Asada Jiro , Cury Leonardo Fadel , Bahniuk Anelize Manuela
Published: March 2023

In Lagoa Vermelha, Brazil, a lagoonal stromatolite and a saltpan microbial mat are investigated to understand the influence of environmental changes on the decomposition of microbial carbonates. The lagoonal stromatolite, composed mainly of magnesian calcite and aragonite, is developed on a dolomite-containing carbonate crust. While most stromatolites are eroded to the water surface level, some smaller, green stromatolites below the water surface retain a domal shape. The domal stromatolite surface is dominated by endolithic cyanobacteria with conspicuous microborings. In addition, microbial aerobic respiration causes carbonate dissolution in darkness, and metazoans grazing the inner surface of the stromatolite excrete fecal pellets. This suggests that the formational stage of lagoonal stromatolites has ceased and they are now decomposing, most likely because of environmental changes in recent years. The microbial mat, which is about 3 cm thick, developed in a saltpan pond precipitating carbonate and gypsum, and it contains quartz, magnesian calcite, aragonite, and gypsum. At the time of the investigation, the population of oxygenic phototrophs is low at the mat surface, and carbonate dissolution, rather than precipitation, is occurring by microbial metabolism deeper in the mat. This suggests that the formation of carbonate in the mat has ceased and is decomposing, probably due to the progressive salinity increase in the salt pan. This examination of two carbonate deposits in Lagoa Vermelha suggests that microbial metabolism is an important process for decomposing microbial carbonates in addition to grazing and microboring, and that environmental changes may alter microbial compositions from carbonate-constructive to carbonate-destructive communities.
Citra-Powder: Inovasi Etnomedisin Jeruk Nipis sebagai Obat Herbal Pereda Batuk Masyarakat Desa Karanglo Kidul
Authors: Khotimah Dwi Fitri , Ramadhani Fahri Eka , Andryansah Laudry Bayu , Anwar Muhammad Khoirul
Published: March 2023

Indonesia adalah salah satu negara yang memiliki kebudayaan yang berlimpah dan diturunkan secara turun temurun oleh nenek moyang. Kebiasaan adat atau budaya yang diwariskan secara turun temurun salah satunya adalah pemanfaatan tumbuhan untuk etnomedisin yang dipercaya mampu mengobati berbagai penyakit secara alami. Tumbuhan yang dimanfaatkan dalam etnomedisin ini salah satunya adalah jeruk nipis (Citrus aurantiifolia). Jeruk nipis dimanfaatkan sebagai obat pereda batuk oleh masyarakat di daerah pedesaan. Salah satunya adalah masyarakat Desa Karanglo Kidul Kec. Jambon. Tujuan dalam penelitian ini adalah untuk menginovasikan jeruk nipis sebagai obat pereda batuk bagi masyarakat dengan praktis dan efisien. Metode yang digunakan dalam penelitian ini adalah R&D yang dikembangkan dengan model 4D. Sehingga didapatkan hasil penelitian yakni produk berupa serbuk jeruk nipis dengan uji organoleptik yakni kemasan yang digunakan sangat menarik, penggunaan produk sangat efektif, rasanya cukup enak, memiliki aroma yang khas dengan tekstur yang mudah larut, serta produk lebih awet dalam penyimpanannya.
Analisis Kemampuan Argumentasi Peserta Didik dalam Menyelesaikan Masalah Berbasis Isu Sosiosaintifik
Authors: Bima Anggara , Fauziah Hanin Niswatul
Published: March 2023

Penelitian ini bertujuan untuk mengetahui kemampuan argumentasi peserta didik dalam menyelesaikan masalah berbasis isu sosisosaintifik. Penelitian ini merupakan penelitian kualitatif dengan jenis studi kasus. Metode pengumpulan data menggunakan tes lisan dan wawancara. Subyek penelitian ini yaitu peserta didik kelas VIII A MTs Muhammadiyah 3 Yanggong yang berjumlah 6 siswa dan guru mata pelajaran IPA, analisis data menggunakan model Miles dan Huberman. Data hasil tes lisan dan wawancara dianalisis secara deskriptif. Hasil penelitian diketahui bahwa kemampuan argumentasi peserta didik dalam menyelesaikan masalah berbasis isu sosiosaintifik berada pada tiga kategori, yaitu tinggi, sedang, dan rendah. Peserta didik yang memiliki kemampuan argumentasi tinggi ada 1, karena dapat menghubungkan 4 indikator kemampuan argumentasi dengan sempurna. Peserta didik yang memiliki kemampuan argumentasi sedang ada 3, peserta didik sudah bisa menghubungkan antara claim, ground, dan backing tetapi pada indikator warrant peserta didik kurang dapat menghubungkan dengan konsep sains. Peserta didik yang memiliki kemampuan argumentasi rendah ada 2, pada indikator claim peserta didik sudah mampu memberikan pernyataan, tetapi peserta didik tidak memberikan kesimpulan dan pada indikator warrant peserta didik tidak menghubungkan dengan konsep sains.
Crystalline monolayer graphdiyne synthesized in the MXene interlayer space
Authors: Li Jiaqiang , Cao Haicheng , Wang Qingxiao , Zhang Hui , Liu Qing , Chen Cailing , Li Guanxing , Kong Ya , Cai Yichen , Shen Jie , Lai Zhiping , Wu Ying , Zhang Jin , Han Yu
Published: March 2023

Graphdiyne (GDY) is an artificial carbon allotrope that is conceptually similar to graphene but composed of sp- and sp2-hybridized carbon atoms. Monolayer GDY (ML-GDY) is predicted to be an ideal two-dimensional (2D) semiconductor material with a wide range of applications. However, its preparation has not been achieved experimentally due to difficulties with synthesis. Here, we report that in-situ polymerization of hexaethynylbenzene within the sub-nanometer interlayer space of MXene can effectively prevent out-of-plane growth or vertical stacking of the material, resulting in crystalline ML-GDY. The subsequent exfoliation process successfully yields free-standing GDY monolayers with micrometer-scale lateral dimensions. The fabrication of field-effect transistor on free-standing ML-GDY makes the first measurement of its electronic properties possible. The measured electrical conductivity (5.1×103 S m-1) and carrier mobility (231.4 cm2 V−1 s−1) at room temperature are remarkably higher than those of the previously reported multilayer GDY materials. The space-constrained synthesis using layered crystals as templates provides a new strategy for preparing 2D materials with precisely controlled layer numbers and long-range structural order.
Risk definers and social discourse of GM foods–a comparative analysis of the People's Daily and the New York Times
Authors: Wang Di , Li Yinyun , Mao Zhifei , He Mengfan , Hon Chitin , Liu Zige
Published: March 2023

Consumers’ understanding of genetic modified foods (GM foods)’s risk and benefits is influenced by the media. Literature on GM foods reports often examined article tones in general, omitting the differences of various social actors’ attitudes. To explore such differences, we conducted a comparative framing analysis of news reports on genetically modified (GM) foods in the United States and China, two large markets of GM foods, to examine (1) the use of frames on GM foods; (2) who has more power in defining the risks of GM foods and (3) the tones towards GM foods in each country. By content analyzing 230 news articles on GM foods from 2014–2019, including 109 from the People’s Daily and 121 from the New York Times, we identified the frames, sources, and tones of each article. We also identified 1,297 sources and their corresponding 1,288 assertions from the 230 news articles to examine the tones of each source. Results showed that the People’s Daily used more treatment responsibility and information frames than the New York Times, while the latter used more conflict, human impact, and causal responsibility frames than the former. While both newspapers published predominantly neutral articles, the New York Times was more likely to publish anti-GM food articles, while the People’s Daily were more likely to publish pro-GM food articles. The New York Times used more diverse sources than the People’s Daily. The former used more anti-GM food sources, while the latter cited more neutral sources.
Fundamental Processes in an Induction Lamp
Authors: Halima Ahlem Ben , Abdelmlak Ben , Sammoudi Bousselham , Araoud Zouhour , Charrada Kamel
Published: March 2023

This paper investigates the kinetic study in an inductive discharge lamp. Numerical simulation of electron density and other parameters that characterize the plasma are discussed and their values are compared with what is expected in inductive plasma and which show a good agreement considering the electron density and the electron temperature values . Such research allows us to understand the relationship between the radial distribution of electronic temperature and the electrical density of the lamp and the frequency of operation. The influence of the current is presented and the performance of the lamp can be found.
Characteristics of soil organic carbon distribution in different economic forests in Gangu County, Gansu Province, China
Authors: Liu Minxia , Mi Jiale , Wang Siyuan , Xiao Shirui , Li Le
Published: March 2023

SOC (Soil organic carbon) is an important indicator of soil nutrients and is essential for maintaining ecosystem functions and preventing land degradation. In this study, five types of economic forest plots were selected as sample plots (Malus pumila, Juglans regia, Zanthoxylum bungeanum, Prunus persica, and Prunus armeniaca) in Gangu County, Gansu Province, and wasteland was used as a control to investigate the changes of soil organic carbon content in 0-100 cm of different economic forests in Gangu County. The results showed that the soil organic carbon content of different economic forests in Gangu County ranged from 9.17 ± 0.15–17.20 ± 0.13 g/kg, and the soil organic carbon content at 0-100 cm depth ranged from high to low: apple (Malus pumila), pepper (Zanthoxylum bungeanum), walnut (Juglans regia), apricot (Prunus armeniaca), peach (Prunus persica) and wasteland. The SOC content of the five forest types and the wasteland showed apparent surface aggregation, with the highest SOC content of 17.20 g/kg for Malus pumila and the lowest SOC content of 12.65 g/kg for the wasteland in the 0–10 cm surface layer; in the 80–100 cm deep layer, the highest SOC content of 14.90 g/kg for Juglans regia and the lowest SOC content of 9.17 g/kg for the wasteland. SWC (Soil water concent, SWC) and S-UE were the most influential factors on the SOC content of Juglans regia; S-CAT (Soil catalase, S-CAT) was the most influential factor on the SOC content of Malus pumila; SBD (Soil bulk density, SBD) was the most influential factor on the SOC content of Prunus armeniaca, Prunus persica, and Zanthoxylum bungeanum; the SOC content of the control wasteland was more influenced by S-CAT and soil fungi. The number of S-CAT and soil fungi more influenced the SOC content of the control wasteland. Overall, the mean SOC content of Malus pumila and Prunus persica in 0-100 cm soils is relatively high among the five economic forest species. Therefore, Malus pumila is the preferred choice for economic forest planting in Gangu County. In the future economic development and ecological restoration practice of Gangu County, it is recommended to plant a mixed forest planting pattern of Malus pumila and Zanthoxylum bungeanum, which is conducive to improving the potential carbon sink function of economic forests in the region.
Assessing the Practical Application of Imaging Mass Cytometry for Visualizing Tumor Immune Microenvironment Heterogeneity
Authors: Huang Shengtao , Zhou Tianhao , Du Shaoqiang , Li Shanhe , Li Ning , Xv Ziqi , Jiang Mengyi , Zhu Ying , Yang Chaoyong , Wang Hongxia , Ding Xianting , Tang Lei , Zang Lijuan
Published: March 2023

BACKGROUND
Analyses of immune cell subsets and immune indices are gaining in importance. By combining molecular probe and immunohistochemical (IHC) analysis with laser ablation, the imaging mass cytometry (IMC) offers spatially high-dimensional in situ measurements in tissue slides with a cell resolution of 1µm. The most critical challenge is how to implement stringent quality control strategies during application of IMC to facilitate reproducible data analysis.
METHODS
We discussed the multi-step experimental processing procedures of IMC, including specimen preparation, panel design, antibody selection, lanthanide metal labeling and data pre-processing workflows by IHC and fluorescent multiplex immunohistochemistry (mIHC) analysis. Based on IMC, we developed a standard operation and well-established agreement for 29 BC patients to identify a structured tumor immune microenvironment.
RESULTS
Metal labeling does not affect the antibodies target specificity (p > 0.05). The 3 µm-thick formalin-fixed and paraffin-embedded (FFPE) and fresh-frozen slides exhibited the least blur (p < 0.01) and the lowest nonspecific adsorption of antibodies (p < 0.01). Detailed analysis of human BC tissues revealed a wide range of differences in the composition and frequency of immune cells within the tumor microenvironment.
CONCLUSIONS
This study offered optimized proposals on procedures for IMC analysis as well as a standard quality control strategy.
Development of a South-South Clinical Research Capacity Strengthening: Institutional Partnership Platform between Cameroon and South Africa
Authors: Ngah Yayah Emerencia
Published: March 2023

Collaborative partnerships are key components to reinforce biomedical and clinical research capacity and are characterized by enormous challenges though important in promoting the institutional exchange of ideas and capacities that potentially fill knowledge and research gaps. An observational analysis was conducted from 2014 to 2022 between the Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa, and the Centre for the Study of Communicable Diseases (CSCCD), Faculty of Medicine and Biomedical Science, University of Yaoundé I, Cameroon. Despite constraints, affiliations with institutes from developed economies are often established within the framework of North-South collaborations. Since 2014, a strong bilateral South-South research partnership between the two institutions has been developed and consolidated for sustainable coexistence. In this paper, we report on qualitative research within our leading collaborative scientists involved in major institutional research, our views on South-South collaboration, and the factors that consolidate our decision-making about joining and participating actively in research networks. Keywords South-South collaboration, Stellenbosch University, South Africa, University of Yaoundé 1, Cameroon.
Legal Regulation of the Competence of Local Self-Government in the Conditions of War in Ukraine
Authors: Lialiuk Oleksii
Published: March 2023

The article is devoted to the issues of competence of local self-government and its bodies during the introduction of martial law, and also to the analysis of amendments to the legislation in the field of local self-government aimed at redistribution of powers in the system of local self-government, and also to the introduction of conditions for "lawful interference" of a number of other entities in the process of exercising by local self-government bodies of their powers. The subject matter of the study is public relations related to ensuring further guarantee of local self-government activities under martial law, inviolability of its powers, and prevention of arbitrariness or usurpation of local self-government powers by public authorities. The purpose of the article is to study the functional and competence sphere of local self-government and to assess the impact on it by public authorities. The study applies systemic-structural and structural-functional, formal-legal, prognostic methods, the method of generalization, as well as the methods of analysis and synthesis. The author examines the current legislation with a view to clearly establishing the conditions for exercising the powers granted to local self-government bodies, as well as the cases, grounds and mechanism under which public authorities may take over the powers of local self-government. The author analyzes the conditions and grounds for early termination of powers of local self-government bodies and the scope of exercise of local self-government powers by military administrations. The legislation is studied to determine the moment and grounds for the transfer of self-governing powers of local self-government to state authorities. It is determined that the impact on the local self-government system by public authorities should be commensurate with the conditions in which the State is currently operating. The author concludes that under martial law, the activities of local self-government should be carried out in accordance with the rules clearly defined by law and should not go beyond the powers granted to them. In case of violation of such rules by the State, appropriate adequate measures should be taken.
Evaluation of the Metabolic Production from the Co-Culture of Saccharicola sp. and Botryosphaeria parva, an Endophytic Fungi Associated with Eugenia jambolana Lam.
Authors: Costa Mayra , Borges Maiara , Chapla Vanessa , Biasetto Carolina , Nascimento Isabele , Bolzani Vanderlan , Araujo Angela
Published: March 2023

A new compound, (6R,7S,2E,4E)-6,7-dihydroxy-4,6-dimethylocta-2,4-dienoic acid (1), together with eight known compounds were isolated from the co-culture of Saccharicola sp. and Botryosphaeria parva, an endophytic fungi associated with Eugenia jambolana Lam. (Myrtaceae) plant species. The structures were elucidated by spectroscopic analysis of the one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) and mass spectrometry (MS) data as well as by comparison with literature data. The bioactivity (antioxidant and antifungal) of the crude EtOAc was evaluated. All crude extracts presented antioxidant activity and only the crude extract from the co-culture was active on the fungus Cladosporium sphaerospermum. This investigation contributed to the knowledge about the metabolic production of two endophytic fungi Saccharicola sp. and Botryosphaeria parva in co-culture, once, until the present date, there are no studies in the literature that report the understanding of the chemical interaction of both grown in the same environment.
A Mini-review on Potentials Proteins/Peptides Applied for Serodiagnosis
of Human Monkeypox Infection and Future Trends
Authors: Moraes Fellipe Alexandre Alves , Duarte Caíque Lopes , Fernandes Gabriel Oliveira , Lisboa Ribeiro Lucas Antônio , Rodrigues Luciana Martins , dos Santos Lacerda Junivania Aparecida , Silva Líria Souza , da Silva Jonatas Oliveira , dos Santos Michelli , da Silva Dantas Alessandra , Chávez-Fumagalli Miguel Angel , Campos-da-Paz Mariana , Giunchetti Rodolfo Cordeiro , Ferraz Coelho Eduardo Antônio , Machado Juliana Martins , Galdino Alexsandro Sobreira
Published: March 2023

Monkeypox is a zoonosis that re-emerged in 2022, generating cases in non-endemic
countries for the disease and creating a public health issue. The rapid increase in the number of cases
kindles a need for quick, inexpensive diagnostic tests for the epidemiological control of the disease.
The high cost of molecular tests can make this control more difficult to access in poorer regions, with
immunological tests being a more viable option. In this mini-review, a search was conducted in the
main databases for peptide and protein options that could be used in the development of serological
diagnostic tests. Nine viable registres were found, and seven were selected (two patents and five
studies). The main studies used the B21R peptide sequence as it is a high immunogenic epitope. In
addition, studies on the improvement of these sequences were also found to avoid cross-reactions
against other viruses of the same family, proposing a rational approach using multiepitope
recombinant proteins. These approaches demonstrated high sensitivity and specificity values and are
seen as viable options for developing new tests. New effective serological testing options, when
combined with awareness, disease surveillance, early diagnosis, and rapid communication, form a set
of key strategies used by health systems to control the spread of the monkeypox virus.
NF-κB as a Potential Target for the Treatment and Prevention of
Mucositis
Authors: Abeesh Prathapan , Guruvayoorappan Chandrasekaran
Published: March 2023

Mucositis is a debilitating and severe side effect of chemotherapy and radiotherapy. It is
responsible for reducing the patient's quality of life and represents a significant economic burden
in oncology. Currently, there is no definitive and definite treatment for this disease. Intracellular
signalling pathways have provided excellent drug development resources, particularly cancer therapeutic
development. In recent decades, active research has been conducted to describe the pathogenesis
of mucositis and the role of nuclear factor-kappa B (NF-κB) signalling pathways in mucositis
development. Insights into the mechanisms of mucositis are creating new approaches for
effective targeted treatment and their success in clinical use. Several studies have concentrated on
elucidating the functional significance of NF-kB activation and its signalling mechanisms in mucositis
in recent decades. Also, evidence indicates that NF-κB is the primary node for the development
and progression of mucositis. Its altered expression is associated with increased mucosal
injury in mucositis. Hence, regulating the activation of NF-κB could be a powerful strategy for the
clinical management of mucositis. Thus, this review examines the role of NF-κB as a potential
therapeutic target for chemotherapy and radiation-induced mucositis therapy.
Mammalian Stable Cell Platforms for Recombinant Adeno-associated Virus
(rAAV) Production: Development Strategies and their Impact on Viral
Productivity
Authors: Fernandes Sofia , Diogo Joana , Coroadinha Ana Sofia
Published: March 2023

Adeno-associated viruses (AAV) are widely used as a recombinant vectors in gene therapy.
AAVs are non-pathogenic. They present reduced cytotoxicity and can transduce both dividing and
non-dividing cells. The existence of different serotypes provides flexibility for targeting different tissues
and organs. Its therapeutic success was already shown by the approval of three products by the
European and American regulatory agencies. To satisfy the high dosage, safety, and reproducibility
required in each clinical trial, production platforms based on stable mammalian cell lines have been
proposed as the best strategy. However, the methodologies employed must be adapted to each cell
line, which often results in distinct productivities. In this article, we review the published and commercially
available mammalian stable cell lines, discussing the key factors that impact viral production
yields, such as integration sites and copy numbers.
MicroRNA-34c-5p Reduces Malignant Properties of Lung Cancer
Cells through Regulation of TBL1XR1/Wnt/β-catenin Signaling
Authors: Lai Weiqiang , Yue Yonghong , Zeng Ganhua
Published: March 2023

Introduction:

Lung cancer is common cancer with high mortality. A growing
number of studies have focused on investigating the regulatory effects of microRNAs
(miRs/miRNAs) during cancer progression. Nevertheless, the biological function of miR-
34c-5p in lung cancer and the underlying mechanism have not been determined. This
study explored the effect of miR-34c-5p on the malignant behaviors of lung cancer cells.

Methods:

In this study, we utilized diverse public databases to obtain differentially
expressed miRNAs. Then, qRT-PCR and western blot were conducted to determine
miR-34c-5p and transducin β-like 1 X-linked receptor 1 (TBL1XR1) expression. Next,
H1299 and H460 cells were transfected with miR-34c-5p-mimic and pcDNA3.1-
TBL1XR1. To examine the anticancer effects of miR-34c-5p, CCK-8, scratch, and
Matrigel-Transwell assays were conducted to test cell viability, migration, and invasion,
respectively. The StarBase database and dual-luciferase reporter gene assay were used
to predict and verify the relationship between miR-34c-5p and TBL1XR1.

Results:

Finally, Wnt/β-catenin signaling- and epithelial-mesenchymal transition (EMT)-
related protein levels were detected using western blot. The results demonstrated that
miR-34c-5p was poorly expressed in lung cancer cells, while TBL1XR1 was highly
expressed. The findings also confirmed the direct interaction between miR-34c-5p and
TBL1XR1. In H1299 and H460 cells, miR-34c-5p overexpression inhibited cell
proliferation, migration, and invasion, Wnt/β-catenin signaling activity, and EMT, while
TBL1XR1 upregulation reversed these effects of miR-34c-5p overexpression.

Conclusion:

These findings illustrated that miR-34c-5p might repress the malignant
behaviors of lung cancer cells via TBL1XR1, providing evidence for miR-34c-5p-based
lung cancer therapy.
Synthesis of Some New 1,2,4-triazole Schiff Bases and their Antibacterial
Activity Studies
Authors: Sim Kooi-Mow , Hoor Yin-Haw , Boo Xin-Lik , Teh Chor-Chin , Lai Shih-Nee , Teh Chor-Pey , Teo Kah-Cheng
Published: March 2023

A new 4-amino-5-mercapto-3-[(1H-indol-3-yl)propyl]-1,2,4-triazole was synthesized from
the fusion of indole-3-butyric acid with thiocarbohydrazide. The reaction of 1,2,4-triazole with a series
of benzaldehydes afforded the corresponding Schiff bases (2a-2s). All the synthesized compounds
were evaluated for their antibacterial activities using a 96-well microbroth dilution assay. The results
of the antibacterial activity revealed that Schiff base 2p with both chloro groups at meta and para positions
of the phenyl exhibited significant inhibition against Bacillus cereus and Staphylococcus aureus
at MIC 9.11 μmol/mL and against Bacillus subtilis spizizenni at 18.20 μmol/mL.
Clinical and Structural Factors Affecting Ablation Outcomes in Atrial
Fibrillation Patients - A Review
Authors: Brilliant Justin , Yadav Ritu , Akhtar Tauseef , Calkins Hugh , Trayanova Natalia , Spragg David
Published: March 2023

Catheter ablation is an effective and durable treatment option for patients with atrial fibrillation (AF). Ablation outcomes vary widely, with optimal results in patients with paroxysmal AF and diminishing results in patients with persistent or long-standing persistent AF. A number of clinical factors including obesity, hypertension, diabetes, obstructive sleep apnea, and alcohol use contribute to AF recurrence following ablation, likely through modulation of the atrial electro-anatomic substrate. In this article, we review the clinical risk factors and the electro-anatomic features that contribute to AF recurrence in patients undergoing ablation for AF.
Development and Characterization of Febuxostat Loaded Floating Beads
as Gastro-retentive Drug Delivery System in the Treatment of Gout: A
Statistical Approach
Authors: Kathota Ravina , Modi Chetna , Thakkar Vaishali , Rana Hardik , Patel Ayushi
Published: March 2023

Background:

Febuxostat is a BCS class-II drug, used in the treatment of gout. However,
because of its lower solubility, a higher and more frequent dose of the drug is required in the treatment.

Objective:

The objective of this research was to develop and evaluate febuxostat-loaded floating beads
as a gastro-retentive drug delivery system (GRDDS) to target drug release up to 24hr in order to enhance
bioavailability.

Methodology:

Gastro-retentive floating beads were formulated using the ionotropic gelation method.
Screening of lipids was carried out based on the shape and texture of floating beads. Drug-excipient
compatibility study was done using DSC analysis. Further optimization of gastro-retentive floating
beads of febuxostat was performed by Box-Behnken design using gelucire 43/01, lactose, and soluplus
as independent variables and %drug entrapment and %drug release after 24 hr as dependent variables.
Evaluation of the optimized batch was performed for in vitro buoyancy, %drug entrapment, %drug release,
FTIR, and SEM study.

Result and Discussion:

In the ANOVA, contour plots, and 3D surface plots, the optimized batch
showed 93.95±0.29 % drug entrapment and 88.14±0.58 % drug release after 24 hr with 98%±1% invitro
buoyancy. Overlay plots and checkpoint batches were accompanied to confirm the optimization.
Polynomial equations proved the positive effect of lipids on drug entrapment and drug release. SEM
images explained porous and microstructures on beads.

Conclusion:

In conclusion, gastro-retentive febuxostat floating beads were successfully developed
and characterized for once a daily dose with enhanced bioavailability and reduced cost of therapy.
Antimicrobial Resistance of Clinical Klebsiella pneumoniae Isolates:
Involvement of AcrAB and OqxAB Efflux Pumps
Authors: AlMatar Manaf , Albarri Osman , Var Işil , Köksal Fatih
Published: March 2023

Background:

Over the last several decades, the AcrAB and OqxAB efflux pumps have been found to cause multidrug resistance (MDR) in various bacteria, most notably Klebsiella pneumoniae. Antibiotic resistance surges with increased expression of the acrAB and oqxAB efflux pumps.

Methods:

In accordance with CLSI guidelines, a disk diffusion test was carried out using 50 K. pneumoniae isolates obtained from various clinical samples. CT was computed in treated samples and compared to a susceptible ciprofloxacin strain (A111). The final finding is presented as the fold change in the target gene's expression in treated samples relative to a control sample (A111), normalized to a reference gene. As CT=0 and 20 equals one, relative gene expression for reference samples is often set to 1.

Results:

The highest rates of resistance were recognized with cefotaxime (100%), cefuroxime (100%), cefepime (100%), levofloxacin (98%), trimethoprim-sulfamethoxazole (80%), and gentamicin (72%), whereas imipenem (34%) had the lowest rates. Overexpression of acrA and acrB, oqxA and oqxB, regulators marA, soxS, and rarA were greater in ciprofloxacin-resistant isolates compared to the reference strain (strain A111). There was also a moderate connection between ciprofloxacin MIC and acrAB gene expression and a moderate connection between ciprofloxacin MIC and oqxAB gene expression.

Conclusion:

This work provides a deeper knowledge of the role of efflux pump genes, particularly acrAB and oqxAB, as well as transcriptional regulators marA, soxS, and rarA, in bacterial resistance to ciprofloxacin.
Thermal Analysis and Biocidal Studies of Copper (II) Soapgroundnut
Complex Containing Urea and Thiourea as a Ligand
Authors: Meena Asha , Sharma Rashmi , Sukhadia Vandana
Published: March 2023

Background:

Studies on the thermal decomposition of synthesized complexes
have great importance for calculating the thermal stability and characterization of copper
(II) soap complexes, and represent new investigations on the solution of environmental
problems.

Aim:

The present research work aims to report new findings in the field of thermogravimetric
analysis and biocidal studies for copper (II) groundnut complexes with urea and thiourea
ligands.

Objective:

The objective of this study was to conduct the kinetic analysis of copper (II)
soap complexes of nitrogen and sulphur-containing ligands with the help of a thermogravimetric
analyser (TGA), as this technique is commonly applied for thermal analysis.

Methods:

In relevance of aforesaid applications, the present work deals with determining
the different thermal degradation steps of newly synthesized copper (II) groundnut urea
complex (CGU) and copper (II) groundnut thiourea complex (CGT) by using Coats-
Redfern, Horowitz-Metzger, Broido, and Piloyan-Novikova equations for determining kinetic
parameters, i.e., the energy of activation (E), rate constant, order of decomposition
reaction, and pre-exponential factor (Z).

Results:

The results obtained from kinetic parameters were used to evaluate the thermodynamic
parameters, i.e., entropy of activation (ΔS), enthalpy of activation (ΔH), and Gibbs
free energy of activation (ΔG), corresponding to the activation by using previously mentioned
equations. Kinetics of degradation for the synthesized complexes in solid state were
studied using thermogravimetric analysis technique (TGA) in nitrogen atmosphere.

Conclusion:

The present study has discussed the biocidal activities of these complexes
against Staphylococcus aureus and an explicit correlation between structure and biological
activity has also been provided.
THE EFFECT OF PRENATAL EXERCISE ON THE INCIDENCE OF POST PARTUM DEPRESSION
Authors:   Department of Midwifery (Diploma) Medicine Faculty, Universitas Sebelas Maret, Surakarta, Indonesia, Pratiwi Dyah
Published: March 2023

Background: The prevalence data for postpartum depression globally reaches 10-15%. In Asia alone, the incidence of postpartum depression reaches 26 - 85%. Meanwhile in Indonesia, the incidence rate is 50-70%. Various interventions are carried out to reduce the prevalence of postpartum depression. Currently, many studies have found the effectiveness of reducing the risk of postpartum depression by preventing routine exercise during pregnancy. Objective: To discuss the effectiveness of exercise during pregnancy compared to the incidence of postpartum depression. Method: This paper involved on referral sources and based on observation by author. Results: Exercise is a non- pharmacological therapeutic effort that is able to reduce stressors in the body, increasing self-confidence in pregnant women who regularly exercise so as to reduce the risk of depression both during pregnancy and after pregnancy. Apart from being related to the mother's own physical factors, there are psychosocial factors that arise when a mother joins regular exercise classes during pregnancy. namely having a strong form of support among pregnant women so that they do not feel alone in facing physical and psychological changes during pregnancy, childbirth, and postpartum There are psychosocial factors that arise when a mother participates in routine exercise classes during pregnancy. namely having a strong form of support among pregnant women so that they do not feel alone in facing physical and psychological changes during pregnancy, childbirth, and postpartum. Conclusion: Psychosocial factors that arise when a mother participates in routine exercise classes during pregnancy having a strong form of support among pregnant women so that they do not feel alone in facing physical and psychological changes during pregnancy, childbirth, and postpartum. Keywords: prenatal, exercise, post-partum depression.
Using Gene Expression Profile to Extract the Biomarker Genes of Cardiovascular Disease
Authors: AlShamlan Hala M.
Published: March 2023

Cardiovascular disease (CVD) is the world’s premier cause of morbidity and death. CVD is a class of heart or blood vessel diseases. CVD contains the coronary artery disease (CAD) such as unstable angina (UA) and myocardial infarction (MI) diseases. Clinicians use additional tools to support clinical evaluation and improve their ability to detect the susceptible patient at threat for CVD. Biomarkers are one such method to identify potential risk persons, rapidly and reliably diagnose disease symptoms that efficiently predict and treat disease. Discovery of MicroRNAs (miRNAs) representing a class of small, non-coding RNA molecules opens interesting opportunities to use the patterns of miRNAs as a biomarker for cardiovascular diseases. The objective of this study is to define miRNA and genes potentially associated with MI. Rothman dataset includes 52 samples of Acute Coronary Syndromes (ACS). including 18 patients with myocardial infarction (MI) and 8 patients with unstable angina (UA). Overall (number of genes selected) candidate ncRNA biomarkers have been defined and a ncRNA-based classifier has been created to predict MI risk which based on 7 ncRNA expression data using vector support machines SVM and decision tree classifiers. The experimental results obtained through applying these mechanisms on the Rothman dataset. The classification model’s performance is evaluated using the V-fold validation and LOOCV methods. The outcome of this search can be used by the drug designer for pathway analysis and CVD treatment decisions.
Women with Postpartum Depression in Kerala: A Survey
Authors: Prasad Pooja
Published: March 2023

The increase in number of Postpartum Depression (PPD) cases in Kerala is increasing day by day. So, a study on the awareness of PPD has become necessary in this scenario. This study tries to quantify the awareness among women in Kerala about PPD and covers how they tackled the issue through various methods used by them to cope with the issue. The study is conducted using an online survey method. A prepared questionnaire was circulated online among 150 young mothers who were born and brought up in Kerala. The questionnaire consisted of eight questions about PPD and baby blues. Each question was provided with options from which the participants had to choose one. The data thus collected were studied and analyzed. The results of the survey were analyzed to arrive at a conclusion. There was active participation from the participants’ side. The results showed that the percentage of participants who were well aware of PPD was very low. The study brought to light that, much attention and activities are needed to solve PPD cases in Kerala. Most women who suffer from PPD are reluctant to seek medical help. This situation can change through proper campaigns and other related activities.
Геттерирование эпитаксиального арсенида индия редкоземельным элементом гольмием
Authors: Куницына Е.В. , Пархоменко Я.А. , Пивоварова А.А. , Яковлев Ю.П.
Published: March 2023

The results of a study of the galvanomagnetic properties of indium arsenide grown by liquid-phase epitaxy are presented. It is shown that the use of the rare earth element holmium in the growth of InAs epitaxial layers makes it possible to reduce the electron concentration by two orders of magnitude to n=2.1•1015 cm−3 at T=77 K. This effect is due to the gettering of shallow background impurities with the formation of their compounds in the melt. With an increase in the holmium content of more than 0.12 mol.% the concentration of current carriers in the material begins to increase, while mobility decreases due to the influence of VAs – Ho donor centers. This method of gettering is promising for obtaining AIIIBV materials with a low concentration of current carriers, which are in demand in the optoelectronic industry.
Acceptability of a Hypothetical Reduction in Routinely Scheduled Clinic Visits Among Patients With History of a Localized Melanoma (MEL-SELF): Pilot Randomized Clinical Trial
Authors: Drabarek Dorothy , Ackermann Deonna , Medcalf Ellie , Bell Katy J L
Published: March 2023

Background

After treatment for a localized melanoma, patients attend routinely scheduled clinics to monitor for new primary or recurrent melanoma. Patient-led surveillance (skin self-examination with patient-performed teledermoscopy) is an alternative model of follow-up that could replace some routinely scheduled visits.

Objective

This study aims to assess the acceptability of a hypothetical reduction in routinely scheduled visits among participants of the Melanoma Self Surveillance (MEL-SELF) pilot randomized clinical trial of patient-led surveillance (intervention) versus usual care (control).

Methods

Patients previously treated for localized melanoma in New South Wales who were participating in the MEL-SELF pilot randomized clinical trial were asked to respond to a web-based questionnaire at baseline and after 6 months on trial. We used mixed methods to analyze the data. The main outcome of interest was the acceptability of a hypothetical reduction in routinely scheduled visits for melanoma surveillance.

Results

Of 100 randomized participants, 87 answered the questionnaire at baseline, 66 answered the questionnaire at 6 months, and 79 provided a free-text explanation at either time point. At 6 months, 33% (17/51) of the control group and 35% (17/49) of the intervention group indicated that a hypothetical reduction in routinely scheduled visits with all melanoma doctors was at least slightly acceptable (difference in proportions –1%, 95% CI –20% to 17%; P=.89). Participants suggested that prerequisites for a reduction in routinely scheduled visits would include that sufficient time had elapsed since the previous diagnosis without a new primary melanoma or recurrence, an unscheduled appointment could be made at short notice if the patient noticed something concerning, their melanoma doctor had suggested reducing their clinic visit frequency, and patients had confidence that patient-led surveillance was a safe and effective alternative. Participants suggested that a reduction in routinely scheduled visits would not be acceptable where they perceived a very high risk of new or recurrent melanoma, low self-efficacy in skin self-examination and in the use of technologies for the patient-led surveillance intervention, and where they had a preference for clinician-led surveillance. Some patients said that a partial reduction to once a year may be acceptable.

Conclusions

Some patients may be receptive to a reduction in routinely scheduled visits if they are assured that patient-led surveillance is safe and effective.

Trial Registration

Australian New Zealand Clinical Trials Registry ACTRN12616001716459; https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=371865&isReview=true; ClinicalTrials.gov NCT03581188; https://clinicaltrials.gov/ct2/show/NCT03581188

International Registered Report Identifier (IRRID)

RR2-10.1001/jamadermatol.2021.4704