bluuebunny/crossref_metadata_2025 huggingface dataset:
Automatic Column Grouping of 3D Steel Frames via Multi-Objective Structural Optimization
Authors: Resende Cláudio , Martha Luiz Fernando , Lemonge Afonso , Hallak Patricia , Carvalho José , Motta Júlia
Published: January 2024

Formulations of structural optimization problems are proposed in this paper to automatically find the best grouping of columns in 3D steel buildings. In these formulations, the conflicting objective functions, minimized simultaneously, are the weight of the structure and the number of different groups of columns. In other words, the smaller the number of different groups of columns, the greater the weight of the structure, and the greater the number of groups, the smaller the structure’s weight. The design variables are the bracing system configuration, column cross-section orientation, and assigned W-shaped profile indices for columns, beams, and braces. The design constraints are the allowable displacements, strength, and geometric considerations. After solving the multi-objective optimization problem, the result is a Pareto front, presenting non-dominated solutions. Three evolutionary algorithms based on differential evolution are adopted in this paper to solve three computational experiments. Even if preliminary groupings of columns are adopted, considering architectural aspects such as the symmetry of the structure, it is possible to discover other interesting structural configurations that will be available to the decision maker, who will be able to make their choices based on the impacts on manufacturing, cutting, transporting, checking and welding.
Multiplex Immunofluorescence Captures Progressive Immune Exhaustion with Advancing Penile Squamous Cell Cancer Stage
Authors: Ionescu Filip , Nguyen Jonathan , Segura Carlos , Paravathaneni Mahati , Grass G. , Johnstone Peter , Zacharias Niki , Pettaway Curtis , Lu Xin , Kim Youngchul , Whiting Junmin , Dhillon Jasreman , Eschrich Steven , Chadha Juskaran , Gullapalli Keerthi , Roman Souza Gabriel , Miyagi Hiroko , Manley Brandon , Spiess Philippe , Chahoud Jad
Published: January 2024

Penile squamous cell carcinoma (PSCC) is a rare and deadly malignancy. Therapeutic advances have been stifled by a poor understanding of disease biology. Specifically, the immune microenvironment is an underexplored component in PSCC and the activity of immune checkpoint inhibitors observed in a subset of patients suggests immune escape may play an important role in tumorigenesis. Herein, we explored for the first time the immune microenvironment of 57 men with PSCC and how it varies with the presence of human papillomavirus (HPV) infection and across tumor stages using multiplex immunofluorescence of key immune cell markers. We observed an increase in the density of immune effector cells in node-negative tumors and a progressive rise in inhibitory immune players such as type 2 macrophages and upregulation of the PD-L1 checkpoint in men with N1 and N2-3 disease. There were no differences in immune cell densities with HPV status.
Integrated Diagnostics of Thyroid Nodules
Authors: Giovanella Luca , Campennì Alfredo , Tuncel Murat , Petranović Ovčariček Petra
Published: January 2024

Thyroid nodules are common findings, particularly in iodine-deficient regions. Our paper aims to revise different diagnostic tools available in clinical thyroidology and propose their rational integration. We will elaborate on the pros and cons of thyroid ultrasound (US) and its scoring systems, thyroid scintigraphy, fine-needle aspiration cytology (FNAC), molecular imaging, and artificial intelligence (AI). Ultrasonographic scoring systems can help differentiate between benign and malignant nodules. Depending on the constellation or number of suspicious ultrasound features, a FNAC is recommended. However, hyperfunctioning thyroid nodules are presumed to exclude malignancy with a very high negative predictive value (NPV). Particularly in regions where iodine supply is low, most hyperfunctioning thyroid nodules are seen in patients with normal thyroid-stimulating hormone (TSH) levels. Thyroid scintigraphy is essential for the detection of these nodules. Among non-toxic thyroid nodules, a careful application of US risk stratification systems is pivotal to exclude inappropriate FNAC and guide the procedure on suspicious ones. However, almost one-third of cytology examinations are rendered as indeterminate, requiring “diagnostic surgery” to provide a definitive diagnosis. 99mTc-methoxy-isobutyl-isonitrile ([99mTc]Tc-MIBI) and [18F]fluoro-deoxy-glucose ([18F]FDG) molecular imaging can spare those patients from unnecessary surgeries. The clinical value of AI in the evaluation of thyroid nodules needs to be determined.
The Versatile Attributes of MGMT: Its Repair Mechanism, Crosstalk with Other DNA Repair Pathways, and Its Role in Cancer
Authors: Fang Qingming
Published: January 2024

O6-methylguanine-DNA methyltransferase (MGMT or AGT) is a DNA repair protein with the capability to remove alkyl groups from O6-AlkylG adducts. Moreover, MGMT plays a crucial role in repairing DNA damage induced by methylating agents like temozolomide and chloroethylating agents such as carmustine, and thereby contributes to chemotherapeutic resistance when these agents are used. This review delves into the structural roles and repair mechanisms of MGMT, with emphasis on the potential structural and functional roles of the N-terminal domain of MGMT. It also explores the development of cancer therapeutic strategies that target MGMT. Finally, it discusses the intriguing crosstalk between MGMT and other DNA repair pathways.
Reactive Gliosis in Neonatal Disorders: Friend or Foe for Neuroregeneration?
Authors: Gargas Justyna , Janowska Justyna , Gebala Paulina , Maksymiuk Weronika , Sypecka Joanna
Published: January 2024

A developing nervous system is particularly vulnerable to the influence of pathophysiological clues and injuries in the perinatal period. Astrocytes are among the first cells that react to insults against the nervous tissue, the presence of pathogens, misbalance of local tissue homeostasis, and a lack of oxygen and trophic support. Under this background, it remains uncertain if induced astrocyte activation, recognized as astrogliosis, is a friend or foe for progressing neonatal neurodevelopment. Likewise, the state of astrocyte reactivity is considered one of the key factors discriminating between either the initiation of endogenous reparative mechanisms compensating for aberrations in the structures and functions of nervous tissue or the triggering of neurodegeneration. The responses of activated cells are modulated by neighboring neural cells, which exhibit broad immunomodulatory and pro-regenerative properties by secreting a plethora of active compounds (including interleukins and chemokines, neurotrophins, reactive oxygen species, nitric oxide synthase and complement components), which are engaged in cell crosstalk in a paracrine manner. As the developing nervous system is extremely sensitive to the influence of signaling molecules, even subtle changes in the composition or concentration of the cellular secretome can have significant effects on the developing neonatal brain. Thus, modulating the activity of other types of cells and their interactions with overreactive astrocytes might be a promising strategy for controlling neonatal astrogliosis.
Cardiomyocyte Differentiation from Mouse Embryonic Stem Cells by WNT Switch Method
Authors: Mensah Isaiah K. , Emerson Martin L. , Tan Hern J. , Gowher Humaira
Published: January 2024

The differentiation of ESCs into cardiomyocytes in vitro is an excellent and reliable model system for studying normal cardiomyocyte development in mammals, modeling cardiac diseases, and for use in drug screening. Mouse ESC differentiation still provides relevant biological information about cardiac development. However, the current methods for efficiently differentiating ESCs into cardiomyocytes are limiting. Here, we describe the “WNT Switch” method to efficiently commit mouse ESCs into cardiomyocytes using the small molecule WNT signaling modulators CHIR99021 and XAV939 in vitro. This method significantly improves the yield of beating cardiomyocytes, reduces number of treatments, and is less laborious.
Sclerostin Alters Tumor Cell Characteristics of Oral Squamous Cell Carcinoma and May Be a Key Player in Local Bone Invasion
Authors: Schirmer Uwe , Schneider Sina Allegra , Khromov Tatjana , Bremmer Felix , Schminke Boris , Schliephake Henning , Liefeith Klaus , Brockmeyer Phillipp
Published: January 2024

Localized jawbone invasion is a milestone in the progression of oral squamous cell carcinoma (OSCC). The factors that promote this process are not well understood. Sclerostin is known to be involved in bone metabolism and there are preliminary reports of its involvement in bone tumors and bone metastasis. To identify a possible involvement of sclerostin in the bone invasion process of OSCC, sclerostin expression was analyzed in vitro in two different human OSCC tumor cell lines by quantitative real-time polymerase chain reaction (qRT-PCR), and the effect of recombinant human (rh)-sclerostin treatment on tumor cell capabilities was evaluated using proliferation, migration, and invasion assays. Undifferentiated human mesenchymal stem cells (hMSCs) were osteogenically differentiated and co-cultured with OSCC tumor cells to demonstrate potential interactions and migration characteristics. Sclerostin expression was evaluated in clinical cases by immunohistochemistry at the OSCC–jawbone interface in a cohort of 15 patients. Sclerostin expression was detected in both OSCC tumor cell lines in vitro and was also detected at the OSCC–jawbone interface in clinical cases. Tumor cell proliferation rate, migration and invasion ability were increased by rh-sclerostin treatment. The migration rate of tumor cells co-cultured with osteogenically differentiated hMSCs was increased. The results presented are the first data suggesting a possible involvement of sclerostin in the bone invasion process of OSCC, which deserves further investigation and may be a potential approach for drug-based tumor therapy.
Camelina sativa Oilseed Cake as a Potential Source of Biopolymer Films: A Chemometric Approach to Synthesis, Characterization, and Optimization
Authors: Šuput Danijela , Pezo Lato , Rakita Slađana , Spasevski Nedeljka , Tomičić Ružica , Hromiš Nevena , Popović Senka
Published: January 2024

In this work, the possibility of obtaining biopolymer films from Camelina sativa oilseed cake (CSoC) at different parameters of the synthesis process was investigated. The pH (values 8, 10, and 12), the temperature (60, 80, and 100 °C), and the concentration of the cake in the film-forming suspension (3, 4, and 5%) were varied. The films obtained were characterized by studying the mechanical, barrier, physico-chemical, structural, and biological properties (antioxidant and antimicrobial). The results showed moderate mechanical properties, indicating the application of these biopolymer materials as coatings. Low values of water vapor permeability (5.1–12.26 g/m2h) and very low transmission in the UV range (less than 2%) indicate good barrier properties. FTIR analysis confirmed the films’ composite structures: peaks related to proteins, polysaccharides, lipids, and cellulose were detected. The biological activity of the obtained CSoC films was pronounced so that they could be used for active packaging. All films have high antioxidant activity, which is more pronounced in samples synthesized at pH 8. The films possess antibacterial properties against S. aureus, while some had an inhibitory effect on S. typhimurium. Standard score analysis showed that the film sample synthetized at pH = 10, temperature = 100 °C, and concentration = 5% had optimal investigated properties.
Decoration of a Glass Surface with AgNPs Using Thio-Derivates for Environmental Applications
Authors: Ilie Cornelia-Ioana , Spoială Angela , Motelica Ludmila , Marinescu Liliana , Dolete Georgiana , Trușcă Doina-Roxana , Oprea Ovidiu-Cristian , Ficai Denisa , Ficai Anton
Published: January 2024

The aim of this study is to decorate a glass surface with silver nanoparticles (AgNPs) and further prove its efficiency in the removal of some thio-derivatives—potential pollutants from water. Therefore, grafting the surface of glass-based platforms with AgNPs will strongly influence their interaction with other substances or molecules. The most commonly used molecules for glass-based platform functionalization/modification are organosilanes. In this case, the main interest is in thioalkyl organosilanes because, after silanization, the thio (-SH) functional groups that have a high affinity for AgNPs can intermediate their binding on the surface. By decorating the glass platforms with AgNPs, these surfaces become active for the adsorption of dyes from wastewater. Certainly, in this case, the dyes must bear -SH groups to ensure a high affinity for these surfaces. Therefore, the desired purpose of this study was to develop glass-based platforms decorated with AgNPs able to bind model molecules—dyes from aqueous media (dithizone—DIT and thioindigo—TIO), with these platforms being potentially used for environmental applications.
Strontium-Doped Mesoporous Bioactive Glass-Loading Bisphosphonates Inhibit Osteoclast Differentiation and Prevent Osteoporosis in Ovariectomized Mice
Authors: Zhou Zhi , Huo Shicheng , Li Zhanchun
Published: January 2024

Postmenopausal osteoporosis, a metabolic bone disease associated with aging that affects bones throughout the body, is emerging as an urgent public health concern and imposes a substantial healthcare burden on society. The clinical application of bisphosphonate, the primary treatment for osteoporosis, is limited owing to the drug’s severe complications. Herein, we investigate the synthesis and utilization of strontium-doped mesoporous bioactive glass loaded with alendronate (ALN@Sr-MBG) as a novel therapeutic agent for osteoporosis, to explore its potential as an alternative to alendronate (ALN). Strontium-doped mesoporous bioactive glass (Sr-MBG) was synthesized using the sol–gel method, while ALN@Sr-MBG was obtained via incorporating Sr-MBG into an alendronate saturated solution. The bioactivities of ALN@Sr-MBG, including biotoxicity, inflammation inhibition, and anti-osteoclast differentiation, were investigated in vitro using CCK-8, flow cytometry, tartrate-resistant acid phosphatase (TRAP) staining, and RT-PCR assays. Animal models were established by surgically removing the ovaries from non-pregnant female C57/BL mice, followed by weekly intraperitoneal injections of ALN@Sr-MBG, ALN, or excipients. After 8 weeks, the mice femurs were extracted and analyzed through micro-CT scanning and hematoxylin-eosi, osteoblastic, and osteogenic staining. These in vitro findings demonstrate that ALN@Sr-MBG displays enhanced biological efficacy compared to ALN in terms of inflammation inhibition, osteogenesis promotion, and osteoclastogenesis inhibition. Furthermore, micro-CT analysis revealed that ALN@Sr-MBG significantly augments bone mineral density (BMD), bone volume fraction (BV/TV), trabecular number (Tb.N), and trabecular thickness (Tb.Th), while reducing trabecular separation (Tb.Sp) and the structural model index (SMI) in mice with ovariectomy-induced osteoporosis. The osteoblast and osteogenic staining results indicate the enhanced bioactivities of ALN@Sr-MBG in promoting bone formation and inhibiting bone resorption compared to ALN. In vitro and in vivo assessments further confirmed that ALN@Sr-MBG exhibits superior anti-osteoporotic bioactivity compared to ALN. This study’s findings confirm the potential of ALN@Sr-MBG as a novel alternative therapy to ALN and a drug candidate for the treatment of osteoporosis.
Hydraulic Performance Optimization of a Submersible Drainage Pump
Authors: Rakibuzzaman Md , Suh Sang-Ho , Roh Hyung-Woon , Song Kyung Hee , Song Kwang Chul , Zhou Ling
Published: January 2024

Small submersible drainage pumps are used to discharge leaking water and rainwater in buildings. In an emergency (e.g., heavy rain or accident), advance monitoring of the flow rate is essential to enable optimal operation, considering the point where the pump operates abnormally when the water level is increased rapidly. Moreover, pump performance optimization is crucial for energy-saving policy. Therefore, it is necessary to meet the challenges of submersible pump systems, including sustainability and pump efficiency. The final goal of this study was to develop an energy-saving and highly efficient submersible drainage pump capable of performing efficiently in emergencies. In particular, this paper targeted the hydraulic performance improvement of a submersible drainage pump model. Prior to the development of driving-mode-related technology capable of emergency response, a way to improve the performance characteristics of the existing submersible drainage pump was found. Disassembling of the current pump followed by reverse engineering was performed instead of designing a new pump. Numerical simulation was performed to analyze the flow characteristics and pump efficiency. An experiment was carried out to obtain the performance, and it was validated with numerical results. The results reveal that changing the cross-sectional shape of the impeller reduced the flow separation and enhanced velocity and pressure distributions. Also, it reduced the power and increased efficiency. The results also show that the pump’s efficiency was increased to 5.56% at a discharge rate of 0.17 m3/min, and overall average efficiency was increased to 6.53%. It was concluded that the submersible pump design method is suitable for the numerical designing of an optimized pump’s impeller and casing. This paper provides insight on the design optimization of pumps.
The Formulation of Dermato-Cosmetic Products Using Sanguisorba minor Scop. Extract with Powerful Antioxidant Capacities
Authors: Tocai (Moţoc) Alexandra-Cristina , Memete Adriana Ramona , Ganea Mariana , Vicaș Laura Graţiela , Gligor Octavia Dorina , Vicas Simona Ioana
Published: January 2024

There has been a significant increase in the use of botanical resources for the formulation of topical products designed for medicinal and cosmetic applications. Sanguisorba minor Scop., a botanical species, exhibits a variety of properties and has significant potential for applications in the field of cosmetics. The aim of this study was to formulate topical preparations, incorporating an extract derived from the plant S. minor Scop. comprising a combination of roots, leaves, and flowers. In the initial phase, a total of seven combinations were prepared using extracts derived from the roots, leaves, and flowers of S. minor Scop. (v/v/v). These combinations were subsequently subjected to evaluation for their antioxidant capacity using four distinct methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), and Trolox equivalent antioxidant capacity (TEAC). An extract of plant organs in a ratio of 1:2:1 (v/v/v), which had a strong antioxidant capacity and demonstrated synergistic effects according to the DPPH, TEAC, and CUPRAC assays (with values of 1.58 ± 0.1, 1.18 ± 0.09, and 1.07 ± 0.07, respectively), was selected for inclusion in three dermato-cosmetic products (hydrogel, emulgel, and cream). All the prepared preparations were evaluated in terms of topical formulation attributes and organoleptic characteristics. The testing of dermato-cosmetic products included assessments of their topical formulation properties and organoleptic characteristics. The hydrogel, emulgel, and cream exhibited varying degrees of stretchability. In addition, a study was carried out to assess the in vitro release of polyphenols from the cosmetic formulations using a Franz diffusion cell system. The results showed that the emulgel containing the extract of S. minor Scop. had the highest and most significant release of polyphenols, with a release rate of 84.39 ± 1.01%. This was followed by the hydrogel and cream, which had release percentages of 80.52 ± 0.89 and 75.88 ± 0.88, respectively, over an 8 h period. Thus, for the first time in the literature, a topical cosmetic product with high antioxidant potential containing S. minor Scop. extract was developed and optimized.
Numerical Simulation of CdTe Crystal Growth Using the Vertical Gradient Freeze Technique Assisted by Axial Low-Frequency Oscillations of the Melt
Authors: Nefedov Oleg , Dovnarovich Alexey , Kostikov Vladimir , Mozhevitina Elena , Bocharnikov Dmitry , Avetissov Igor
Published: January 2024

The problem of intensification of the melt crystal growth process has been analyzed using CdTe as an actual material. Numerical simulation of 100 mm diameter CdTe crystal growth using the VGF technique has been carried out. The heat–mass transfer was controlled by introducing low-frequency oscillating baffle into the melt, which is a so-called axial vibrational control (AVC) technique. The baffle configuration has been optimized to destroy solid “tails”, which were formed near the crucible walls at high cooling rates due to the low thermoconductivity and the corresponding latent heat. Analysis of CdTe homogeneity range showed that during fast crystal cooling, Te micro precipitations were formed, resulting from the decay of oversaturated Cd-rich nonstoichiometric solid solution during the Bridgman crystal growth technique. After full crystallization, a VGF-grown CdTe crystal stays inside the phase field of the high-temperature wurtzite polymorph. This makes it possible to go through the polymorph transition without Te micro-precipitating using the advantages of the VGF-specific feature of very slow cooling.
Analyzing the Impact of Simulated Nitrogen Deposition on Stoichiometric Properties and Yield of Ma Bamboo (Dendrocalamus latiflorus Munro) Shoots, Leaves, and Soil Substrate
Authors: Lin Yuwei , Zheng Suyun , Su Jianlin , Rong Jundong , He Tianyou , Zheng Yushan , Chen Liguang
Published: January 2024

The growth dynamics of Ma bamboo (Dendrocalamus latiflorus Munro) are intricately linked to nitrogen availability, a pivotal nutrient. Escalating global nitrogen deposition, primarily driven by anthropogenic factors, is reshaping nutrient fluxes and productivity within forest and bamboo ecosystems. Such alterations bear significant implications for the growth equilibrium and yields of rapid-growth species such as Ma bamboo, thereby influencing their sustainable management strategies. This investigation delves into the responses of Ma bamboo under varying nitrogen deposition scenarios (0 g·clump−1, 11.2 g·clump−1, 13.5 g·clump−1, and 22.5 g·clump−1), examining stoichiometric attributes in bamboo shoots, leaves, and soil across distinct growth phases. Our empirical findings reveal that in the early growth stage, nitrogen enrichment markedly augmented N and P concentrations in the foliage and shoots, alongside a corresponding enhancement in soil P content. This was paralleled by a substantial reduction in the C:N ratio in leaves and the C:P ratio in shoots and soil, indicating an amplified uptake of P and N in both plant and soil matrices. During the middle stage, all nitrogen treatments boosted nitrogen levels across various plant tissues, while concurrently, soil C content exhibited a notable decline with increased nitrogen supplementation. In the late stage, leaf and soil N content continued to ascend; however, alterations in C content in both soil and leaves were not pronounced. Contrastingly, N and P levels in shoots showed a gradual decrement. Yield assessments disclosed that during the early stage, the N3 treatment (22.5 g·clump−1) not only delayed shoot emergence by 14 days but also surged the yield by 115.87% in comparison to the control (CK). In the late stage, the N2 treatment (13.5 g·clump−1) extended emergence duration by 10 days, with the yield apex under N3 treatment (22.5 g·clump−1) evidencing a 116.67% yield augmentation over CK. In summation, this study elucidates the stoichiometric balancing and distribution strategies within the plant–soil system of Ma bamboo, investigating its adaptability and responsive feedback to diverse nitrogen deposition gradients. This research contributes to a deeper understanding of plant nutrient adaptation mechanisms in the context of nitrogen deposition, enriches the discourse on plant population stoichiometry, and offers valuable insights and scientific underpinnings for broader-scale community or ecosystem stoichiometry studies.
Forest Cover and Locality Regulate Response of Watershed Discharge to Rainfall Variability in Caribbean Region
Authors: Gao Qiong , Yu Mei
Published: January 2024

Reforestation often occurs when the economy shifts from agriculture to industry and services such as tourism. However, there is a lack of coherent knowledge and investigation about the impact of reforestation in the tropics on hydrological variability as well as flood risks. It is unclear how changes in forest cover and pattern will affect flood risks and watershed response to future altered rainfall intensity. This study uses the Soil Water Assessment Tool (SWAT+) to simulate the impact of reforestation, the locality of forest, and the concentrated rainfall on the hydrology of the largest watershed in Puerto Rico. SWAT+ is a computer model simulating watershed hydrology driven by meteorological input and the characteristics of soils and land use. We hypothesized that increased forest cover, especially at low elevation range, would reduce flood risk and that reduced rain days while maintaining the mean annual rainfall invariant would increase stream discharge variability. We found that reforestation significantly reduced large discharges but increased small discharges; that forest at low elevation tended to reduce large and extreme discharges in comparison with forest at high elevation; and that more concentrated rainfall not only increased the rainfall variability but also increased the discharge variability. However, both the impact of shifting forest locality and the response of watershed to altered rainfall intensity strongly depended on geophysical factors such as ranges of elevation and slope. Moving forests to lower elevation in subbasins with steeper slopes showed a stronger reduction in extreme discharges than in subbasins with flatter slopes. On the other hand, subbasins with steeper slopes tended to response more strongly to more concentrated rainfall with greater increase in discharge variability than subbasins with flatter slopes. To cope with future increased climate variability, our results favor reforestation at lower elevation for watershed with large elevation ranges and steep slopes.
Predicting Wood Density Using Resistance Drilling: The Effect of Instrument and Operator
Authors: Gendvilas Vilius , Lee David J. , Kain Dominic P. , Kumar Chandan , Downes Geoff M. , Lausberg Marco , Harrington Jonathan J.
Published: January 2024

The IML PD series Resi is an instrument designed to measure the drilling resistance of wood. Use of the IML PD series Resi instrument is rapidly becoming a routine method among Australian forest plantation growers for wood quality assessments. The major driver in the commercial uptake of the IML Resi is that it is fast, cheap, and sufficiently precise for commercial use, particularly when the data are processed through a user-friendly, web-based processor. This study examined whether the accuracy of wood density predictions was impacted by the use of different Resi tools and different operators, which has never been examined before. Seven Resi instruments were evaluated across six sites with two operators on plantation-grown Southern Pine (Pinus caribaea var. hondurensis (Sénéclauze) or hybrids between Pinus elliottii var. elliottii (Engelm) × P. caribaea var. hondurensis) trees. Two types of Resi instruments were used (IML Resi PD-400 and IML Resi PD-500), and all had been recently serviced or were new. The instruments were operated by experienced operators. Constant sampling conditions of feed speed 200 cm/min and 3500 RPM were used. The order of instrument use, and hence the order of operator assessment at each site, was randomized. The variance between Resi instruments was small. The measured mean basic density of 50 mm outerwood cores across all plots was 542 kg/m3, while Resi-predicted basic density varied among instruments between 535 and 547 kg/m3. One Resi instrument underpredicted basic density by 9 kg/m3 and another overpredicted by 5 kg/m3. The operator had no effect on the basic density prediction. Resi PD400 or PD500 instruments gave similar basic density predictions.
Unraveling the Formation Mechanism of Egg’s Unique Flavor via Flavoromics and Lipidomics
Authors: Zhou Zheng , Cui Shuang , Che Jing , Zhang Yuying , Zhou Dayong , Huang Xuhui , Qin Lei
Published: January 2024

Egg products after thermal treatment possess a unique flavor and are favored by consumers. In this study, the key aroma-active compounds of egg yolk products and their formation mechanism during thermal treatment were investigated. The volatile aroma compounds in egg yolks were monitored using an electronic nose, gas chromatography-mass spectrometry (GC–MS) and gas chromatography–olfactometry–mass spectrometry (GC–O–MS), and the lipid molecular species were explored using ultra-high-performance liquid chromatography– mass spectrometry with a Q-Exactive HF-X Orbitrap (UPLC-Q-Exactive HF-X). A total of 68 volatile compounds were identified. Boiled eggs mainly derived their flavor from hexanal, 2-pentyl-furan, 2-butanone, 3-methyl-butanal and heptane. Meanwhile, fried eggs relied mainly on 14 compounds, the most important of which were 2-ethyl-3-methyl-pyrazine, 3-ethyl-2,5-dimethyl-pyrazine, 2-ethyl-3,5-dimethyl-pyrazine, nonanal and 2,3-diethyl-5-methyl-pyrazine, providing a baked and burnt sugar flavor. A total of 201 lipid molecules, belonging to 21 lipid subclasses, were identified in egg yolks, and 13 oxidized lipids were characterized using a molecular network. Phosphoethanolamines (PEs) containing polyunsaturated fatty acids were the primary flavor precursors contributing to the development of egg yolks’ flavor, participating in lipid oxidation reactions and the Maillard reaction and regulating the production of aldehydes and pyrazine compounds. This study provides reference and guidance for the development of egg yolk flavor products.
Advertising and Eco-Labels as Influencers of Eco-Consumer Attitudes and Awareness—Case Study of Ecuador
Authors: Carrión-Bósquez Nelson , Veas-González Iván , Naranjo-Armijo Franklin , Llamo-Burga Mary , Ortiz-Regalado Oscar , Ruiz-García Wilfredo , Guerra-Regalado Wilson , Vidal-Silva Cristian
Published: January 2024

This study examined the impact of green advertising and eco-labels on the attitudes and environmental awareness of millennials purchasing eco-friendly products in shopping centers across Ecuador. The research utilized a quantitative, correlational, cross-sectional methodology with 430 millennials participating. A 20-item survey was administered face-to-face at shopping centers in Quito and Guayaquil, Ecuador. The validity of the research model was established through Confirmatory Factor Analysis (CFA) and Structural Equation Modeling (SEM), employing SPSS 20 and AMOS 24 for statistical evaluations. Findings reveal that green advertising significantly shapes environmental attitudes (β: 0.245) and awareness (β: 0.110), as well as directly influences the purchasing behavior (β: 0.154) towards green products. While eco-labels do not exert a direct effect on purchasing behavior (β: 0.128), they significantly inform attitudes (β: 0.406) and ecological awareness (β: 0.277) of millennials who purchase organic products. This paper is among the pioneering research to delineate the correlation between green advertising elements and the purchasing patterns of green products among millennials in a developing nation. It concludes that marketing strategies centered on green advertising and eco-labels do affect millennials’ attitudes and environmental consciousness, but only advertising has a direct impact on purchasing behaviors, contrary to eco-labels. The research bears social significance as it affirms that millennials are attentive to environmental issues and are actively engaged in promoting sustainability.
Impact of Cooking Methods on Phenolic Acid Composition, Antioxidant Activity, and Starch Digestibility of Chinese Triticale Porridges: A Comparative Study between Atmospheric Pressure and High Pressure Boiling
Authors: Li Hua , Mao Yurong , Ma Danni , Li Hua , Liu Ruixin , Siriamornpun Sirithon
Published: January 2024

Water boiling under atmospheric pressure (CAP) and water boiling under high pressure (CHP) are two popular domestic cooking methods for Chinese porridge making. In this study, we aimed to evaluate the effects of these two methods on the phenolic acid composition, antioxidant activity, and starch digestibility of triticale porridges. The contents of total free and total bound phenolic acids in the CHP sample were 1.3 and 1.6 times higher than those in the CAP counterpart, respectively, although the DPPH and ABTS values of these two samples were comparable. CAP induced more small pieces of starch than CHP, and the gelatinization enthalpy was 19% higher in the CHP sample than that in the CAP. Both cooking methods increased the starch digestibility, while the CHP sample (58.84) showed a lower GI than the CAP (61.52). These results may promote the application of triticale in health-promoting staple foods.
Intelligent Biosensors Promise Smarter Solutions in Food Safety 4.0
Authors: Chen Yuehua , Wang Yicheng , Zhang Yiran , Wang Xin , Zhang Chen , Cheng Nan
Published: January 2024

Food safety is closely related to human health. However, the regulation and testing processes for food safety are intricate and resource-intensive. Therefore, it is necessary to address food safety risks using a combination of deep learning, the Internet of Things, smartphones, quick response codes, smart packaging, and other smart technologies. Intelligent designs that combine digital systems and advanced functionalities with biosensors hold great promise for revolutionizing current food safety practices. This review introduces the concept of Food Safety 4.0, and discusses the impact of intelligent biosensors, which offer attractive smarter solutions, including real-time monitoring, predictive analytics, enhanced traceability, and consumer empowerment, helping improve risk management and ensure the highest standards of food safety.
nFeS Embedded into Cryogels for High-Efficiency Removal of Cr(VI): From Mechanism to for Treatment of Industrial Wastewater
Authors: Xu Peng , Jiang Shaojun
Published: January 2024

Most studies have focused on complex strategies for materials preparation instead of industrial wastewater treatment due to emergency treatment requirements for metal pollution. This study evaluated sodium polyacrylate (PSA) as a carbon skeleton and FeS as a functional material to synthesize PSA-nFeS material. The characteristics and interactions of PSA-nFeS composites treated with hexavalent chromium were analyzed by means of various techniques, such as scanning electron microscopy-energy dispersive X-ray spectrometry (SEM-EDS), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectrometry (FTIR), and atomic absorption spectroscopy (AAS). Adsorption-coupled reduction was observed to be the predominant mechanism of Cr(VI) removal. The feasibility of PSA-nFeS composites in reducing toxicity and removing of Cr(VI) from real effluents was investigated through column studies and material properties evaluation. The continuous column studies were conducted using tannery effluents to optimize feed flow rates, initial feed Cr(VI) concentration, and column bed height. The results revealed that PSA-nFeS composites are ideal for filling materials in portable filtration devices due to their lightweight and compact size.
Complete Mitochondrial Genome and Phylogenetic Analysis of Tarsiger indicus (Aves: Passeriformes: Muscicapidae)
Authors: Lan Guanwei , Yu Jiaojiao , Liu Juan , Zhang Yue , Ma Rui , Zhou Yanshan , Zhu Biqing , Wei Wei , Liu Jiabin , Qi Guilan
Published: January 2024

Tarsiger indicus (Vieillot, 1817), the White-browed Bush Robin, is a small passerine bird widely distributed in Asian countries. Here, we successfully sequenced its mitogenome using the Illumina Novaseq 6000 platform (Illumina, San Diego, CA, USA) for PE 2 × 150 bp sequencing. Combined with other published mitogenomes, we conducted the first comprehensive comparative mitogenome analysis of Muscicapidae birds and reconstructed the phylogenetic relationships between Muscicapidae and related groups. The T. indicus mitogenome was 16,723 bp in size, and it possessed the typical avian mitogenome structure and organization. Most PCGs of T. indicus were initiated strictly with the typical start codon ATG, while COX1 and ND2 were started with GTG. RSCU statistics showed that CUA, CGA, and GCC were relatively high frequency in the T. indicus mitogenome. T. cyanurus and T. indicus shared very similar mitogenomic features. All 13 PCGs of Muscicapidae mitogenomes had experienced purifying selection. Specifically, ATP8 had the highest rate of evolution (0.13296), whereas COX1 had the lowest (0.01373). The monophylies of Muscicapidae, Turdidae, and Paradoxornithidae were strongly supported. The clade of ((Muscicapidae + Turdidae) + Sturnidae) in Passeriformes was supported by both Bayesian Inference and Maximum likelihood analyses. The latest taxonomic status of many passerine birds with complex taxonomic histories were also supported. For example, Monticola gularis, T. indicus, and T. cyanurus were allocated to Turdidae in other literature; our phylogenetic topologies clearly supported their membership in Muscicapidae; Paradoxornis heudei, Suthora webbiana, S. nipalensis, and S. fulvifrons were formerly classified into Muscicapidae; we supported their membership in Paradoxornithidae; Culicicapa ceylonensis was originally classified as a member of Muscicapidae; our results are consistent with a position in Stenostiridae. Our study enriches the genetic data of T. indicus and provides new insights into the molecular phylogeny and evolution of passerine birds.
The Pathophysiology of Inherited Renal Cystic Diseases
Authors: Satariano Matthew , Ghose Shaarav , Raina Rupesh
Published: January 2024

Renal cystic diseases (RCDs) can arise from utero to early adulthood and present with a variety of symptoms including renal, hepatic, and cardiovascular manifestations. It is well known that common RCDs such as autosomal polycystic kidney disease and autosomal recessive kidney disease are linked to genes such as PKD1 and PKHD1, respectively. However, it is important to investigate the genetic pathophysiology of how these gene mutations lead to clinical symptoms and include some of the less-studied RCDs, such as autosomal dominant tubulointerstitial kidney disease, multicystic dysplastic kidney, Zellweger syndrome, calyceal diverticula, and more. We plan to take a thorough look into the genetic involvement and clinical sequalae of a number of RCDs with the goal of helping to guide diagnosis, counseling, and treatment.
Nurses’ Workplace Perceptions in Southern Germany—Job Satisfaction and Self-Intended Retention towards Nursing
Authors: Sommer Domenic , Wilhelm Sebastian , Wahl Florian
Published: January 2024

Our cross-sectional study, conducted from October 2022 to January 2023, aims to assess post-COVID job satisfaction, crucial work dimensions, and self-reported factors influencing nursing retention. Using an online survey, we surveyed 2572 nurses in different working fields in Bavaria, Germany. We employed a quantitative analysis, including a multivariable regression, to assess key influence factors on nursing retention. In addition, we evaluated open-ended questions via a template analysis to use in a joint display. In the status quo, 43.2% of nurses were not committed to staying in the profession over the next 12 months. A total of 66.7% of our surveyed nurses were found to be dissatisfied with the (i) time for direct patient care. Sources of dissatisfaction above 50% include (ii) service organization, (iii) documentation, (iv) codetermination, and (v) payment. The qualitative data underline necessary improvements in these areas. Regarding retention factors, we identified that nurses with (i) older age, (ii) living alone, (iii) not working in elder care, (iv) satisfactory working hours, (v) satisfactory career choice, (vi) career opportunities, (vii) satisfactory payment, and (viii) adequate working and rest times are more likely to remain in the profession. Conversely, dissatisfaction in (ix) supporting people makes nurses more likely to leave their profession and show emotional constraints. We uncovered a dichotomy where nurses have strong empathy for their profession but yearn for improvements due to unmet expectations. Policy implications should include measures for younger nurses and those in elderly care. Nevertheless, there is a need for further research, because our research is limited by potential bias from convenience sampling, and digitalization will soon show up as a potential solution to improve, e.g., documentation and enhanced time for direct patient time.
Cross-Cultural Adaptation and Validation of the Arabic Version of the Physical Activity Scale for Individuals with Physical Disabilities in Saudi Arabia (PASIPD-AR)
Authors: Alhumaid Majed M. , Said Mohamed A. , Adnan Yuhanis , Khoo Selina
Published: January 2024

This study aimed to cross-culturally adapt and validate the Arabic version of the Physical Activity Scale for Individuals with Physical Disabilities (PASIPD) with Saudi Arabian participants. The study encompassed four distinct stages: (i) translation and subsequent back-translation; (ii) a preliminary assessment aimed at evaluating the quality of the translated scale; (iii) an assessment of the reliability of the measures employed; and (iv) a comprehensive examination of the validity of the measures. A sample of Saudi Arabian participants with physical disabilities (N = 206) took part, ranging in age from 18 to 70 years old, with an average age of 39.56 years and a standard deviation of 12.16. The findings obtained from the reliability tests indicated a notable level of internal consistency and stability. Experts and confirmatory factor analysis were employed to establish the face, content, and construct validity. The findings of the assessment of the Arabic version of PASIPD demonstrated a satisfactory degree of reliability and validity, rendering it suitable for implementation within the Saudi Arabian setting.
Impact of Physical Activity on Autonomy and Quality of Life in Individuals with Down Syndrome: A Systematic Review
Authors: Muñoz-Llerena Antonio , Ladrón-de-Guevara Laura , Medina-Rebollo Daniel , Alcaraz-Rodríguez Virginia
Published: January 2024

Down syndrome (DS) is the most common genetic alteration in humans, resulting from the trisomy of chromosome 21. Individuals with DS are characterized by physical traits and limitations related to intellectual functioning and the development of motor skills. People with DS tend to have lower levels of physical activity (PA) than the general population, despite its benefits for health and quality of life, which could be caused by barriers such as the lack of adapted programs or knowledge on how to adapt them. Therefore, this systematic review aimed to examine the impact of physical activity or sports programs on autonomy and quality of life in individuals with DS. Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines were followed to search four databases (Scopus, Web of Science, PubMed, and SportDiscus), adhering to the population, intervention, comparison, and outcome strategy. A total of 13 studies were selected that followed different training programs (combined training, adapted football, technology-based, pulmonary training, gymnastics and dance, water-based, and whole body vibration). Most of the interventions provided benefits for autonomy or quality of life (physical, psychological, cognitive, emotional, and social) in people with DS. In conclusion, the benefits of physical activity and sports programs adapted to people with DS are positive.
Emerging Role of [18F]FLT PET/CT in Lymphoid Malignancies: A Review of Clinical Results
Authors: Nappi Anna Giulia , Santo Giulia , Jonghi-Lavarini Lorenzo , Miceli Alberto , Lazzarato Achille , La Torre Flavia , Dondi Francesco , Gorica Joana
Published: January 2024

Fluorine-18 fluorodeoxyglucose ([18F]FDG) is nowadays the leading positron emission tomography (PET) tracer for routine clinical work-ups in hematological malignancies; however, it is limited by false positive findings. Notably, false positives can occur in inflammatory and infective cases or in necrotic tumors that are infiltrated by macrophages and other inflammatory cells. In this context, 3′-deoxy-3′-[18F]fluorothymidine ([18F]FLT) has been shown to be a promising imaging biomarker of hematological malignant cell proliferation. In this review, a total of 15 papers were reviewed to collect literature data regarding the clinical application of [18F]FLT PET/CT in hematological malignancies. This imaging modality seems to be a suitable tool for noninvasive assessment of tumor grading, also showing a correlation with Ki-67 immunostaining. Moreover, [18F]FLT PET/CT demonstrated high sensitivity in detecting aggressive lymphoma lesions, especially when applying a standardized uptake value (SUV) cutoff of 3. At baseline, the potential of [18F]FLT imaging as a predictive tool is demonstrated by the low tracer uptake in patients with a complete response. However, its use is limited in evaluating bone diseases due to its high physiological uptake in bone marrow. Interim [18F]FLT PET/CT (iFLT) has the potential to identify high-risk patients with greater precision than [18F]FDG PET/CT, optimizing risk-adapted therapy strategies. Moreover, [18F]FLT uptake showed a greater ability to differentiate tumor from inflammation compared to [18F]FDG, allowing the reduction of false-positive findings and making the first one a more selective tracer. Finally, FLT emerges as a superior independent predictor of PFS and OS compared to FDG and ensures a reliable early response assessment with greater accuracy and predictive value.
Bioavailability of Cd in Plantago weldenii and Sonchus oleraceus Plants: The Effects of a Humic and Fulvic Acids-Based Biostimulant
Authors: Grammenou Aspasia , Petropoulos Spyridon A. , Antoniadis Vasileios
Published: January 2024

Cadmium (Cd) contamination poses a major threat to plants and human health, as well as to ecosystem services. Biostimulants provide a promising eco-friendly solution to enhance the phytoremediation of Cd-contaminated soils. We examined the influence of a humic and fulvic acids-based biostimulant on two plant species, e.g., Plantago weldenii and Sonchus oleraceus (common sowthistle), in a soil spiked with Cd at 45 mg kg−1. The aim of this study was to determine whether a biostimulant can potentially affect Cd mobility in soil and absorption in plant tissues. We found that the biostimulant significantly decreased Cd bioavailability (recorded as DTPA extractability) in the soil where Plantago was grown from 17.57 to 13.12 mg kg−1, probably due to the Cd immobilization effect of the added biostimulant. However, the biostimulant had the opposite effect in the soil where S. oleraceus was grown (Cd-DTPA significantly increased from 10.13 to 13.03 mg kg−1). S. oleraceus was found to have accumulated higher Cd concentrations in its aerial parts, resulting in a soil-to-plant transfer value close to 1 and root-to-shoot translocation value well above 1. These two indices exhibited the potential of S. oleraceus to be used as hyperaccumulator in Cd-contaminated soils, while P. weldenii behaved rather as a Cd excluder. These findings highlight the complex dynamics of added biostimulants and Cd behavior in soil and plants. We recognize the need for further research so that the mechanisms dictating Cd behavior after biostimulant application can be better elucidated.