bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing the Role of Food Related Lifestyle in Predicting Intention towards Edible Insects
Authors: Verneau Fabio , La Barbera Francesco , Amato Mario , Riverso Roberta , Grunert Klaus G.
Published: September 2020

Although recent literature has shown that switching to an insect-based diet could provide several relevant advantages—from a nutritional, environmental, economic and ecological point of view—the potential growth of insects as everyday food is still unclear. Despite a growing literature on consumer acceptance and product preference for insect-based food, a segmentation of this future and possible market has never been proposed. Therefore, in the present paper, a market segmentation based on the Food Related Lifestyle Scale (FRLS), was performed in order to predict consumers’ willingness to eat (WTE) edible insects. Moreover, the role of perceived behavioural control is taken into account. Results shows that the novelty and benefits of insect consumption have generated much interest in edible insects amongst consumers belonging to the Rational cluster, who showed the highest intention to introduce insects in their diet, thus confirming the presence of a niche of “early adopters”. In addition, perceived behavioural control was the major driver of intention. Implications for attempts to encourage people to incorporate insect-based foods into their diet are discussed, with special reference to the role of marketing campaigns.
An Analysis and Comparison of Survival and Functional Outcomes in Oropharyngeal Squamous Cell Carcinoma Patients Treated with Concurrent Chemoradiation Therapy within City of Hope Cancer Center Sites
Authors: Pharaon Rebecca , Chung Samuel , Amini Arya , Maghami Ellie , Chowdhury Arnab , Vora Nayana , Chang Sue , Kang Robert , Gernon Thomas , Hansen Kelly , Kelly Christina , Ackerman Denise , Vora Lalit , Sampath Sagus , Massarelli Erminia
Published: September 2020

Oropharyngeal squamous cell carcinoma (OPSCC) is a subset of head and neck cancers that can arise due to human papillomavirus (HPV) infection. We designed a retrospective analysis to determine differences in outcomes of OPSCC patients treated at City of Hope (COH) Cancer Center’s main campus versus selected satellite sites with COH-associated faculty and facilities. Patients diagnosed with OPSCC and treated with concurrent chemoradiation therapy (n = 94) were identified and included in the study. Patients underwent treatment at the COH main campus site (n = 50) or satellite sites (n = 44). The majority of patients were Caucasian, male, and diagnosed with p16 positive stage IV locally advanced OPSCC by AJCC 7th edition. Most patients completed their prescribed cumulative radiation therapy dose and had a complete response to treatment. No significant difference in overall survival and progression-free survival was observed between the main campus and the satellite sites. Our study demonstrates successful treatment completion rates as well as comparable recurrence rates between the main campus and COH-associated satellite sites. A trend toward significant difference in feeding tube dependency at 6-months was observed. Differences in feeding tube placement and dependency rates could be addressed by the establishment of on-site supportive services in satellite sites.
Polysomnography and Nocturia Evaluations after Uvulopalatopharyngoplasty for Obstructive Sleep Apnea Syndrome
Authors: Tsou Yung-An , Chou Eric Chieh-Lung , Shie Dung-Yun , Lee Ming-Jeng , Chang Wen-Dien
Published: September 2020

A higher incidence rate of nocturia in patients with obstructive sleep apnea (OSA) has been observed. We investigated the differences in clinical examinations between OSA patients with and without nocturia, and further compared those with successful and unsuccessful uvulopalatopharyngoplasty (UPPP). This retrospective study enrolled 103 patients with OSA undergoing UPPP. Patients were diagnosed with OSA by following the 2018 American Academy of Sleep Medicine (AASM) Scoring Manual Version 2.5. Patients were divided into two groups depending on if they urinated more than twice per night. The medical data of body mass index (BMI), nocturia frequency per night, apnea–hypopnea index (AHI), Epworth Sleepiness Scale (ESS), International Prostatic Symptom Score (IPSS), and Overactive Bladder Symptom Score (OABSS) were analyzed before and after uvulopalatopharyngoplasty (UPPP) surgery. All of the measurements were compared between successful and unsuccessful surgery in the non-nocturia or nocturia groups, respectively. Fifty patients (41 males and nine females) without nocturia were assigned to group 1, and 53 patients (43 males and 10 females) with nocturia were assigned to group 2. Nocturia frequency and post-surgery AHI in group 2 were significantly higher than those in group 1 (p < 0.05). Significant decreases in IPSS and OABSS were observed in the successful surgery subgroup of group 2 (p < 0.05). A significant decrease in post-surgery AHI was observed between unsuccessful and successful surgery in patients with nocturia (p < 0.05), but not in the non-nocturia group (p > 0.05). Although AHI had a significant correlation to nocturia frequency in all OSA patients before UPPP, no significant correlation between AHI reduction and nocturia frequency was found. UPPP appeared to be an effective treatment for nocturia associated with OSA. OSA should be taken into consideration for patients who complain of nocturia syndrome. The relationship of AHI reduction and nocturia improvement after OSA treatment with UPPP is still unclear. In addition, it is necessary to establish the existence of nocturia in patients with OSA, as a result of its high prevalence in OSA patients. UPPP could reduce the symptoms of OSA and could also contribute to a reduction of nocturia even in the unsuccessful surgery group.
Absence of BCL-2 Expression Identifies a Subgroup of AML with Distinct Phenotypic, Molecular, and Clinical Characteristics
Authors: haes Inke De , Dendooven Amélie , Mercier Marie Le , Puylaert Pauline , Vermeulen Katrien , Kockx Mark , Deiteren Kathleen , Maes Marie-Berthe , Berneman Zwi , Anguille Sébastien
Published: September 2020

Acute myeloid leukemia (AML) is a hematologic malignancy characterized by the rapid and uncontrolled clonal growth of myeloid lineage cells in the bone marrow. The advent of oral, selective inhibitors of the B-cell leukemia/lymphoma-2 (BCL-2) apoptosis pathway, such as venetoclax, will likely induce a paradigm shift in the treatment of AML. However, the high cost of this treatment and the risk of additive toxicity when used in combination with standard chemotherapy represent limitations to its use and underscore the need to identify which patients are most—and least—likely to benefit from incorporation of venetoclax into the treatment regimen. Bone marrow specimens from 93 newly diagnosed AML patients were collected in this study and evaluated for BCL-2 protein expression by immunohistochemistry. Using this low-cost, easily, and readily applicable analysis method, we found that 1 in 5 AML patients can be considered as BCL-2−. In addition to a lower bone marrow blast percentage, this group exhibited a favorable molecular profile characterized by lower WT1 expression and underrepresentation of FLT3 mutations. As compared to their BCL-2+ counterparts, the absence of BCL-2 expression was associated with a favorable response to standard chemotherapy and overall survival, thus potentially precluding the necessity for venetoclax add-on.
Mesoscale Ocean Feature Identification in the North Aegean Sea with the Use of Sentinel-3 Data
Authors: Spondylidis Spyros , Topouzelis Konstantinos , Kavroudakis Dimitris , Vaitis Michail
Published: September 2020

The identification of oceanographic circulation related features is a valuable tool for environmental and fishery management authorities, commercial use and institutional research. Remote sensing techniques are suitable for detection, as in situ measurements are prohibitively costly, spatially sparse and infrequent. Still, these imagery applications require a certain level of technical and theoretical skill making them practically unreachable to the immediate beneficiaries. In this paper a new geospatial web service is proposed for providing daily data on mesoscale oceanic feature identification in the North Aegean Sea, produced by Sentinel-3 SLSTR Sea Surface Temperature (SST) imagery, to end users. The service encompasses an automated process for: raw data acquisition, interpolation, oceanic feature extraction and publishing through a webGIS application. Level-2 SST data are interpolated through a Co-Kriging algorithm, involving information from short term historical data, in order to retain as much information as possible. A modified gradient edge detection methodology is then applied to the interpolated products for the mesoscale feature extraction. The resulting datasets are served according to the Open Geospatial Consortium (OGC) standards and are available for visualization, processing and download though a dedicated web portal.
An Underwater Image Enhancement Algorithm Based on MSR Parameter Optimization
Authors: Hu Kai , Zhang Yanwen , Lu Feiyu , Deng Zhiliang , Liu Yunping
Published: September 2020

The quality of underwater images is often affected by the absorption of light and the scattering and diffusion of floating objects. Therefore, underwater image enhancement algorithms have been widely studied. In this area, algorithms based on Multi-Scale Retinex (MSR) represent an important research direction. Although the visual quality of underwater images can be improved to some extent, the enhancement effect is not good due to the fact that the parameters of these algorithms cannot adapt to different underwater environments. To solve this problem, based on classical MSR, we propose an underwater image enhancement optimization (MSR-PO) algorithm which uses the non-reference image quality assessment (NR-IQA) index as the optimization index. First of all, in a large number of experiments, we choose the Natural Image Quality Evaluator (NIQE) as the NR-IQA index and determine the appropriate parameters in MSR as the optimization object. Then, we use the Gravitational Search Algorithm (GSA) to optimize the underwater image enhancement algorithm based on MSR and the NIQE index. The experimental results show that this algorithm has an excellent adaptive ability to environmental changes.
Reframing Psychiatry for Precision Medicine
Authors: Torres Elizabeth B.
Published: September 2020

The art of observing and describing behaviors has driven diagnosis and informed basic science in psychiatry. In recent times, studies of mental illness are focused on understanding the brain’s neurobiology but there is a paucity of information on the potential contributions from peripheral activity to mental health. In precision medicine, this common practice leaves a gap between bodily behaviors and genomics that we here propose to address with a new layer of inquiry that includes gene expression on tissues inclusive of brain, heart, muscle-skeletal and organs for vital bodily functions. We interrogate gene expression on human tissue as a function of disease-associated genes. By removing genes linked to disease from the typical human set, and recomputing gene expression on the tissues, we can compare the outcomes across mental illnesses, well-known neurological conditions, and non-neurological conditions. We find that major neuropsychiatric conditions that are behaviorally defined today (e.g., autism, schizophrenia, and depression) through DSM-observation criteria have strong convergence with well-known neurological conditions (e.g., ataxias and Parkinson’s disease), but less overlap with non-neurological conditions. Surprisingly, tissues majorly involved in the central control, coordination, adaptation and learning of movements, emotion and memory are maximally affected in psychiatric diagnoses along with peripheral heart and muscle-skeletal tissues. Our results underscore the importance of considering both the brain–body connection and the contributions of the peripheral nervous systems to mental health.
Refining the Tiered Approach for Mapping and Assessing Ecosystem Services at the Local Scale: A Case Study in a Rural Landscape in Northern Germany
Authors: Perennes Marie , Campagne C. Sylvie , Müller Felix , Roche Philip , Burkhard Benjamin
Published: September 2020

Spatially explicit assessments of ecosystem services (ES) potentials are a key component in supporting a sustainable land use management. The ES matrix method is a commonly used approach as it allows for a comparably fast, comprehensible and accessible ES assessment. As it is often based on land use/land cover data (LULC) with no spatial variability, a main critique is that the results fail to assess spatial variability at landscape levels, which limits the reliability of the outputs for spatial planning applications. By using the case study area of Bornhöved in northern Germany, we analyzed three assessment methods that combine expert judgments, LULC data with different resolutions and ecosystem condition indicators, in order to find the required resolution and data for ES assessment and mapping at a local scale. To quantify map discrepancies, we used the structural similarity index (SSIM) and analyzed the differences in local mean, variance and covariance between the maps. We found that using different spatial resolutions led to a relatively small difference in the outcomes, in which regulation and maintenance services are more affected than the other services categories. For most regulation, maintenance and cultural ES, our results indicate that assessments based only on LULC proxies are not suitable for a local quantitative assessment of ES, as they cannot sufficiently cover the spatial heterogeneity of ES capacities that arise from different ecosystem conditions.
Microstructural Investigations of Ni-Based Superalloys by Directional Solidification Quenching Technique
Authors: Wittenzellner Tobias , Sumarli Shieren , Schaar Helge , Wang Fu , Ma Dexin , Bührig-Polaczek Andreas
Published: September 2020

The improvement of the mechanical properties of Ni-based superalloys is achieved in most cases by modifying the chemical composition. Besides that, the processing can be modified to optimize the as-cast microstructure with regard to the mechanical properties. In this context, the present study highlights the solidification mechanism of several Ni-based superalloys by conducting experiments using a modified, laboratory-scale Bridgman-Stockbarger furnace. In that context, the single-crystal rods are partially melted, directionally solidified and quenched sequentially. Several characterization methods are applied to further analyze the influence of the alloying elements and the variation of the withdrawal rate on the as-cast microstructure. Four stages of solidification are distinguished whereby the morphology observed in the different stages mainly depends on the cooling rate and the local concentration of the carbide forming elements. The effect of carbide precipitation and the effect on the as-cast microstructure is investigated by employing energy dispersive X-ray spectrometry (EDX) and electron backscatter diffraction (EBSD) analysis techniques. A local polycrystalline structure is observed in the single-crystal system as consequence of the influence of the carbon content and the cooling rate. The present work aims to develop strategies to suppress the formation of the polycrystalline structure to maintain the single-crystal microstructure.
Selective Laser Melting of 18NI-300 Maraging Steel
Authors: Król Mariusz , Snopiński Przemysław , Hajnyš Jiří , Pagáč Marek , Łukowiec Dariusz
Published: September 2020

In the present study, 18% Ni 300 maraging steel powder was processed using a selective laser melting (SLM) technique to study porosity variations, microstructure, and hardness using various process conditions, while maintaining a constant level of energy density. Nowadays, there is wide range of utilization of metal technologies and its products can obtain high relative density. A dilatometry study revealed that, through heating cycles, two solid-state effects took place, i.e., precipitation of intermetallic compounds and the reversion of martensite to austenite. During the cooling process, one reaction took place (i.e., martensitic transformation), which was confirmed by microstructure observation. The improvements in the Rockwell hardness of the analyzed material from 42 ± 2 to 52 ± 0.5 HRC was improved as a result of aging treatment at 480 °C for 5 h. The results revealed that the relative density increased using laser speed (340 mm/s), layer thickness (30 µm), and hatch distance (120 µm). Relative density was found approximately 99.3%. Knowledge about the influence of individual parameters in the SLM process on porosity will enable potential manufacturers to produce high quality components with desired properties.
High Breakdown Voltage and Low Buffer Trapping in Superlattice GaN-on-Silicon Heterostructures for High Voltage Applications
Authors: Tajalli Alaleh , Meneghini Matteo , Besendörfer Sven , Kabouche Riad , Abid Idriss , Püsche Roland , Derluyn Joff , Degroote Stefan , Germain Marianne , Meissner Elke , Zanoni Enrico , Medjdoub Farid , Meneghesso Gaudenzio
Published: September 2020

The aim of this work is to demonstrate high breakdown voltage and low buffer trapping in superlattice GaN-on-Silicon heterostructures for high voltage applications. To this aim, we compared two structures, one based on a step-graded (SG) buffer (reference structure), and another based on a superlattice (SL). In particular, we show that: (i) the use of an SL allows us to push the vertical breakdown voltage above 1500 V on a 5 µm stack, with a simultaneous decrease in vertical leakage current, as compared to the reference GaN-based epi-structure using a thicker buffer thickness. This is ascribed to the better strain relaxation, as confirmed by X-Ray Diffraction data, and to a lower clustering of dislocations, as confirmed by Defect Selective Etching and Cathodoluminescence mappings. (ii) SL-based samples have significantly lower buffer trapping, as confirmed by substrate ramp measurements. (iii) Backgating transient analysis indicated that traps are located below the two-dimensional electron gas, and are related to CN defects. (iv) The signature of these traps is significantly reduced on devices with SL. This can be explained by the lower vertical leakage (filling of acceptors via electron injection) or by the slightly lower incorporation of C in the SL buffer, due to the slower growth process. SL-based buffers therefore represent a viable solution for the fabrication of high voltage GaN transistors on silicon substrate, and for the simultaneous reduction of trapping processes.
Towards Distributed Recycling with Additive Manufacturing of PET Flake Feedstocks
Authors: Little Helen A. , Tanikella Nagendra G. , J. Reich Matthew , Fiedler Matthew J. , Snabes Samantha L. , Pearce Joshua M.
Published: September 2020

This study explores the potential to reach a circular economy for post-consumer Recycled Polyethylene Terephthalate (rPET) packaging and bottles by using it as a Distributed Recycling for Additive Manufacturing (DRAM) feedstock. Specifically, for the first time, rPET water bottle flake is processed using only an open source toolchain with Fused Particle Fabrication (FPF) or Fused Granular Fabrication (FGF) processing rather than first converting it to filament. In this study, first the impact of granulation, sifting, and heating (and their sequential combination) is quantified on the shape and size distribution of the rPET flakes. Then 3D printing tests were performed on the rPET flake with two different feed systems: an external feeder and feed tube augmented with a motorized auger screw, and an extruder-mounted hopper that enables direct 3D printing. Two Gigabot X machines were used, each with the different feed systems, and one without and the latter with extended part cooling. 3D print settings were optimized based on thermal characterization, and both systems were shown to 3D print rPET directly from shredded water bottles. Mechanical testing showed the importance of isolating rPET from moisture and that geometry was important for uniform extrusion. The mechanical strength of 3D-printed parts with FPF and inconsistent flow is lower than optimized fused filament, but adequate for a wide range of applications. Future work is needed to improve consistency and enable water bottles to be used as a widespread DRAM feedstock.
Influence of Variable Radius of Cutting Head Trajectory on Quality of Cutting Kerf in the Abrasive Water Jet Process for Soda–Lime Glass
Authors: Sutowska Marzena , Kapłonek Wojciech , Pimenov Danil Yurievich , Gupta Munish Kumar , Mia Mozammel , Sharma Shubham
Published: September 2020

The main innovation of this article is the determination of the impact of curvature of a shape cut out in a brittle material using an abrasive water jet (AWJ) process as an important factor of the machined surfaces. The curvature of a shape, resulting from the size of the radius of the cutting head trajectory, is one of the key requirements necessary for ensuring the required surface quality of materials shaped by the abrasive water jet process, but very few studies have been carried out in this regard. An important goal of the experimental studies carried out here and presented in this work was to determine its influence on the quality of the inner and outer surfaces of the cutting kerf. This goal was accomplished by cutting the shape of a spiral in soda–lime glass. For such a shape, the effect of radius of the trajectory of the cutting head on selected parameters of the surface texture of the inner surface of the cutting kerf (IS) and the outer surface of the cutting kerf (OS) was studied. The obtained results of the experimental studies confirmed that the effect of the curvature of the cut shape is important from the point of view of the efficiency of the glass-based brittle material-cutting process using AWJ. Analyses of the surface textures of the areas located in the upper part of the inner and outer surfaces separated by the use of AWJ machining showed that the OS surfaces are characterized by worse technological quality compared with IS surfaces. Differences in the total height of surface irregularities (given by St amplitude parameter), determined on the basis of the obtained results of the measurements of both surfaces of the cutting kerf, were as follows: ΔStr = 50 = 0.6 μm; ΔStr = 35 = 1 μm; ΔStr = 15 = 1.3 μm. The analysis of values measured in areas located in the more sensitive zone of influence of the AWJ outflow proved that the total height of irregularities (St) of the OS was higher. Differences in the total heights of irregularities for inner and outer surfaces of the cutting kerf were as follows: ΔStr = 50 = 2.1 μm; ΔStr = 35 = 3 μm; ΔStr = 15 = 14.1 μm, respectively. The maximum difference in the total heights of irregularities (St), existing between the surfaces considered in a special case (radius 15 mm), was almost 20%, which should be a sufficient condition for planning cutting operations, so as to ensure the workpiece is shaped mainly by internal surfaces.
Nanoparticles Synthesis in Wet-Operating Stirred Media: Investigation on the Grinding Efficiency
Authors: Trofa Marco , D’Avino Gaetano , Fabiano Bruno , Vocciante Marco
Published: September 2020

The use of nanomaterials, thanks to their peculiar properties and versatility, is becoming central in an increasing number of scientific and engineering applications. At the same time, the growing concern towards environmental issues drives the seeking of alternative strategies for a safer and more sustainable production of nanoparticles. Here we focus on a low-energy, magnetically-driven wet milling technique for the synthesis of metal nanoparticles starting from a bulky solid. The proposed approach is simple, economical, sustainable, and provides numerous advantages, including the minimization of the nanoparticles air dispersion and a greater control over the final product. This process is investigated by experiments and discrete element method simulations to reproduce the movement of the grinding beads and study the collision dynamics. The effect of several parameters is analyzed, including the stirring bar velocity, its inclination, and the grinding bead size, to quantify the actual frequency, energy, and angle of collisions. Experiments reveal a non-monotonous effect of the stirring velocity on the abrasion efficiency, whereas numerical simulations highlight the prevalent tangential nature of collisions, which is only weakly affected by the stirring velocity. On the other hand, the stirring velocity affects the collision frequency and relative kinetic energy, suggesting the existence of an optimal parameters combination. Although a small variation of the stirring bar length does not significantly affect the collision dynamics, the use of grinding beads of different dimensions offers several tuning opportunities.
Mechanical Characterization of Multiwalled Carbon Nanotubes: Numerical Simulation Study
Authors: Sakharova Nataliya A. , Pereira André F. G. , Antunes Jorge M. , Fernandes José V.
Published: September 2020

The elastic properties of armchair and zigzag multiwalled carbon nanotubes were investigated under tensile, bending, and torsion loading conditions. A simplified finite element model of the multiwalled carbon nanotubes, without taking into account the van der Waals interactions between layers, was used to assess their tensile, bending, and torsional rigidities and, subsequently, Young’s and shear moduli. Relationships between the tensile rigidity and the squares of the diameters of the outer and inner layers in multiwalled carbon nanotubes, and between the bending and torsional rigidities with the fourth powers of the diameters of the outer and inner layers, were established. These relationships result in two consistent methods, one for assessment to the Young’s modulus of armchair and zigzag multiwalled carbon nanotubes, based on tensile and bending rigidities, and the other to evaluate shear modulus using tensile, bending, and torsional rigidities. This study provides a benchmark regarding the determination of the mechanical properties of nonchiral multiwalled carbon nanotubes by nanoscale continuum modeling approach.
Plasticity and Formability of Annealed, Commercially-Pure Aluminum: Experiments and Modeling
Authors: Ha Jinjin , Fones Johnathon , Kinsey Brad L. , Korkolis Yannis P.
Published: September 2020

The plasticity and formability of a commercially-pure aluminum sheet (AA1100-O) is assessed by experiments and analyses. Plastic anisotropy of this material is characterized by uniaxial and plane-strain tension along with disk compression experiments, and is found to be non-negligible (e.g., the r-values vary between 0.445 and 1.18). On the other hand, the strain-rate sensitivity of the material is negligible at quasistatic rates. These results are used to calibrate constitutive models, i.e., the Yld2000-2d anisotropic yield criterion as the plastic potential and the Voce isotropic hardening law. Marciniak-type experiments on a fully-instrumented hydraulic press are performed to determine the Forming Limit Curve of this material. Stereo-type Digital Image Correlation is used, which confirms the proportional strain paths induced during stretching. From these experiments, limit strains, i.e., the onset of necking, are determined by the method proposed by ISO, as well as two methods based on the second derivative. To identify the exact instant of necking, a criterion based on a statistical analysis of the noise that the strain signals have during uniform deformation versus the systematic deviations that necking induces is proposed. Finite element simulation for the Marciniak-type experiment is conducted and the results show good agreement with the experiment.
Cobalt Chromium Molybdenum Surface Modifications Alter the Osteogenic Differentiation Potential of Human Mesenchymal Stem Cells
Authors: Lohberger Birgit , Eck Nicole , Glaenzer Dietmar , Lichtenegger Helga , Ploszczanski Leon , Leithner Andreas
Published: September 2020

Surface roughness on orthopedic implant materials has been shown to be highly influential on the behavior of osteogenic cells. Mesenchymal stem and progenitor cells (MSPCs) migrate to the interface, adhere, proliferate, and differentiate into osteoblasts, which subsequently form bone matrix. Modifications of the implant surfaces should accelerate this process and improve biocompatibility. In this study, five surface topographies on cobalt chromium molybdenum (CoCrMo) were engineered to examine the influence on MSPCs. Scanning electron microscopy revealed significant differences in the morphology of untreated CoCrMo discs in comparison with CoCrMo with a titanium nitride (TiN) coating, polished and porous coated CoCrMo surfaces, and CoCrMo with a pure titanium (cpTi) coating. Elemental analysis was performed using energy-dispersive X-ray spectroscopy (EDX). Human primary MSPCs were expanded from tissue samples of spongiosa bone and characterized according to the criteria of the International Society for Cellular Therapy. The characteristic phenotype of MSPC was confirmed by flow cytometry and multilineage differentiation. Alcaline phosphatase and osteopontin expression increased significantly in all groups about 5-fold and 10-fold, respectively, in comparison to the undifferentiated controls. The porous coated surface showed a reduced expression of osteogenic markers. Due to the osteogenic differentiation, the expression of integrin α5β1, which is particularly important for cell-material contact, increased 4–7-fold. In the dynamic process of bone biology, MSPCs cultured and differentiated on cpTi, showed significant upregulation of IL6 and leptin.
On Minimal and Maximal Hyperidealsin n-ary Semihypergroups
Authors: Daengsaen Jukkrit , Leeratanavalee Sorasak , Davvaz Bijan
Published: September 2020

The concept of j-hyperideals, for all positive integers 1≤j≤n and n≥2, in n-ary semihypergroups, is a generalization of the concept of left, lateral and right hyperideals in ternary semihypergroups. In this paper, we first introduce the concept of j-(0-)simple n-ary semihypergroups and discuss their related properties through terms of j-hyperideals. Furthermore, we characterize the minimality and maximality of j-hyperideals in n-ary semihypergroups and establish the relationships between the (0-)minimal, maximal j-hyperideals and the j-(0-)simple n-ary semihypergroups. Our main results are to extend and generalize the results on semihypergroups and ternary semihypergroups. Moreover, a related question raised by Petchkaew and Chinram is solved.
Two Nested Limit Cycles in Two-Species Reactions
Authors: Nagy Ilona , Romanovski Valery G. , Tóth János
Published: September 2020

We search for limit cycles in the dynamical model of two-species chemical reactions that contain seven reaction rate coefficients as parameters and at least one third-order reaction step, that is, the induced kinetic differential equation of the reaction is a planar cubic differential system. Symbolic calculations were carried out using the Mathematica computer algebra system, and it was also used for the numerical verifications to show the following facts: the kinetic differential equations of these reactions each have two limit cycles surrounding the stationary point of focus type in the positive quadrant. In the case of Model 1, the outer limit cycle is stable and the inner one is unstable, which appears in a supercritical Hopf bifurcation. Moreover, the oscillations in a neighborhood of the outer limit cycle are slow-fast oscillations. In the case of Model 2, the outer limit cycle is unstable and the inner one is stable. With another set of parameters, the outer limit cycle can be made stable and the inner one unstable.
Anti-Acne Effects of Cembrene Diterpenoids from the Cultured Soft Coral Sinularia flexibilis
Authors: Chen Li-Wei , Chung Hsuan-Lien , Wang Ching-Chiung , Su Jui-Hsin , Chen Yu-Ju , Lee Chia-Jung
Published: September 2020

Acne is a skin disease common in adolescents and increasingly common in the adult population. The major pathologic events of acne vulgaris include increased sebum production, retention hyperkeratosis, carrying commensal skin microbiota, and inflammation. In recent years, more than 10,000 compounds have been isolated and identified from marine organisms. The aim of this study was to discover the potential anti-acne activity of fraction 9 + 10 (SF-E) of Sinularia flexibilis extract and six cembrene diterpenoids. We found that the SF-E significantly reduced Cutibacterium acnes-induced edema in Wistar rat ears. The cembrene diterpenoids including 11-dehydrosinulariolide (SC-2), 3,4:8,11-bisepoxy-7-acetoxycembra-15(17)-en-1,12-olide (SC-7), and sinularin (SC-9) reduced nitric oxide (NO) production with 50% inhibitory concentration of 5.66 ± 0.19, 15.25 ± 0.25, and 3.85 ± 0.25 μM, respectively, and inducible NO synthase expression in RAW 264.7 cells. Moreover, treatment with SC-2, SC-7, and SC-9 significantly suppressed lipopolysaccharide- and heat-killed C. acnes-induced expression of proteins involved in mitogen-activated protein kinase pathway in both RAW 264.7 and HaCaT cells. After treatment with SC-2, SC-7, and SC-9, over-proliferation of HaCaT cells was significantly terminated. In summary, SC-2, SC-7, and SC-9 showed anti-inflammatory effects in RAW 264.7 cells, suggesting that these cembrene diterpenoids obtained from S. flexibilis are natural marine products with potential anti-acne activities.
One Step Forward towards the Development of Eco-Friendly Antifouling Coatings: Immobilization of a Sulfated Marine-Inspired Compound
Authors: Vilas-Boas Cátia , Carvalhal Francisca , Pereira Beatriz , Carvalho Sílvia , Sousa Emília , Pinto Madalena M. M. , Calhorda Maria José , Vasconcelos Vitor , Almeida Joana R. , Silva Elisabete R. , Correia-da-Silva Marta
Published: September 2020

Marine biofouling represents a global economic and ecological challenge and few eco-friendly antifouling agents are available. The aim of this work was to establish the proof of concept that a recently synthesized nature-inspired compound (gallic acid persulfate, GAP) can act as an eco-friendly and effective antifoulant when immobilized in coatings through a non-release strategy, promoting a long-lasting antifouling effect. The synthesis of GAP was optimized to provide quantitative yields. GAP water solubility was assessed, showing values higher than 1000 mg/mL. GAP was found to be stable in sterilized natural seawater with a half-life (DT50) of 7 months. GAP was immobilized into several commercial coatings, exhibiting high compatibility with different polymeric matrices. Leaching assays of polydimethylsiloxane and polyurethane-based marine coatings containing GAP confirmed that the chemical immobilization of GAP was successful, since releases up to fivefold lower than the conventional releasing systems of polyurethane-based marine coatings were observed. Furthermore, coatings containing immobilized GAP exhibited the most auspicious anti-settlement effect against Mytilus galloprovincialis larvae for the maximum exposure period (40 h) in laboratory trials. Overall, GAP promises to be an agent capable of improving the antifouling activity of several commercial marine coatings with desirable environmental properties.
Hydrogen Selective SiCH Inorganic–Organic Hybrid/γ-Al2O3 Composite Membranes
Authors: Kubo Miwako , Mano Ryota , Kojima Misako , Naniwa Kenichi , Daiko Yusuke , Honda Sawao , Ionescu Emanuel , Bernard Samuel , Riedel Ralf , Iwamoto Yuji
Published: September 2020

Solar hydrogen production via the photoelectrochemical water-splitting reaction is attractive as one of the environmental-friendly approaches for producing H2. Since the reaction simultaneously generates H2 and O2, this method requires immediate H2 recovery from the syngas including O2 under high-humidity conditions around 50 °C. In this study, a supported mesoporous γ-Al2O3 membrane was modified with allyl-hydrido-polycarbosilane as a preceramic polymer and subsequently heat-treated in Ar to deliver a ternary SiCH organic–inorganic hybrid/γ-Al2O3 composite membrane. Relations between the polymer/hybrid conversion temperature, hydrophobicity, and H2 affinity of the polymer-derived SiCH hybrids were studied to functionalize the composite membranes as H2-selective under saturated water vapor partial pressure at 50 °C. As a result, the composite membranes synthesized at temperatures as low as 300–500 °C showed a H2 permeance of 1.0–4.3 × 10−7 mol m−2 s−1 Pa−1 with a H2/N2 selectivity of 6.0–11.3 under a mixed H2-N2 (2:1) feed gas flow. Further modification by the 120 °C-melt impregnation of low molecular weight polycarbosilane successfully improved the H2-permselectivity of the 500 °C-synthesized composite membrane by maintaining the H2 permeance combined with improved H2/N2 selectivity as 3.5 × 10−7 mol m−2 s−1 Pa−1 with 36. These results revealed a great potential of the polymer-derived SiCH hybrids as novel hydrophobic membranes for purification of solar hydrogen.
Comparison of Productivity and Fecal Microbiotas of Sows in Commercial Farms
Authors: Uryu Haruka , Tsukahara Takamitsu , Ishikawa Hiromichi , Oi Munetaka , Otake Satoshi , Yamane Itsuro , Inoue Ryo
Published: September 2020

Sow productivity, that is, the number of weaned piglets per sow per year, depends on their health status. The gut microbiota is considered a crucial factor in the health of pigs and may affect sow productivity. In the present study, we aimed to investigate the relationship between productivity and the fecal microbiotas of sows in different farms. Feces of sows were collected from 18 farms (10 samples/farm). A total of 90 fecal samples of high-reproductive performance farms were labeled as group H, and 90 fecal samples from low-reproductive performance farms were labeled as group L. Fecal microbiotas were analyzed by 16S rRNA metagenomics, and the organic acids and putrefactive metabolites of the microbiotas were measured. β-diversity was significantly different between groups H and L (p < 0.01), and the relative abundances of 43 bacterial genera, including short-chain fatty acid-producing and fiber-degrading bacteria such as Ruminococcus, Fibrobacter and Butyricicoccus, significantly differed between groups (p < 0.05). In addition, the concentrations of acetate, propionate and n-butyrate were significantly higher in group H than in group L (p < 0.05). In conclusion, sow productivity in farms was likely associated with the compositions of the fecal microbiotas.
Impact of Hepatitis B Virus Genetic Variation, Integration, and Lymphotropism in Antiviral Treatment and Oncogenesis
Authors: Lau Keith C.K. , Burak Kelly W. , Coffin Carla S.
Published: September 2020

Chronic Hepatitis B Virus (HBV) infection poses a significant global health burden. Although, effective treatment and vaccinations against HBV are available, challenges still exist, particularly in the development of curative therapies. The dynamic nature and unique features of HBV such as viral variants, integration of HBV DNA into host chromosomes, and extrahepatic reservoirs are considerations towards understanding the virus biology and developing improved anti-HBV treatments. In this review, we highlight the importance of these viral characteristics in the context of treatment and oncogenesis. Viral genotype and genetic variants can serve as important predictive factors for therapeutic response and outcomes in addition to oncogenic risk. HBV integration, particularly in coding genes, is implicated in the development of hepatocellular carcinoma. Furthermore, we will discuss emerging research that has identified various HBV nucleic acids and infection markers within extrahepatic sites (lymphoid cells). Intriguingly, the presence of hepatocellular carcinoma (HCC)-associated HBV variants and viral integration within the lymphoid cells may contribute towards the development of extrahepatic malignancies. Improved understanding of these HBV characteristics will enhance the development of a cure for chronic HBV infection.
Valganciclovir as Add-On to Standard Therapy in Secondary Glioblastoma
Authors: Stragliotto Giuseppe , Pantalone Mattia Russel , Rahbar Afsar , Söderberg-Nauclér Cecilia
Published: September 2020

Patients with glioblastoma have a very poor prognosis despite aggressive therapeutic strategies. Cytomegalovirus has been detected in >90% of glioblastoma tumors. This virus can affect tumor progression and may represent a novel glioblastoma therapy target. We report, here, a retrospective survival analysis of patients with secondary glioblastoma who were treated with the anti-viral drug valganciclovir at Karolinska University Hospital in Stockholm. We performed survival analyses of eight patients with secondary glioblastoma who were treated with a standard dose of valganciclovir as an add-on to second-line therapy after their disease progression to glioblastoma. Thirty-six patients with secondary glioblastoma admitted during the same time period who received similar treatment and care served as contemporary controls. The patients treated with valganciclovir showed an increased median overall survival after progression to glioblastoma compared with controls (19.1 versus 12.7 months, p = 0.0072). This result indicates a potential positive effect of valganciclovir in secondary glioblastoma, which is in agreement with our previous observation that valganciclovir treatment improves the outcomes of patients with newly diagnosed glioblastoma. Larger randomized studies are warranted to prove this hypothesis.
Novel Insights into the Effect of Pythium Strains on Rapeseed Metabolism
Authors: Bělonožníková Kateřina , Vaverová Kateřina , Vaněk Tomáš , Kolařík Miroslav , Hýsková Veronika , Vaňková Radomíra , Dobrev Petre , Křížek Tomáš , Hodek Ondřej , Čokrtová Kateřina , Štípek Adam , Ryšlavá Helena
Published: September 2020

Pythium oligandrum is a unique biological control agent. This soil oomycete not only acts as a mycoparasite, but also interacts with plant roots and stimulates plant defense response via specific elicitors. In addition, P. oligandrum can synthetize auxin precursors and stimulate plant growth. We analyzed the secretomes and biochemical properties of eleven Pythium isolates to find a novel and effective strain with advantageous features for plants. Our results showed that even closely related P. oligandrum isolates significantly differ in the content of compounds secreted into the medium, and that all strains secrete proteins, amino acids, tryptamine, phenolics, and hydrolytic enzymes capable of degrading cell walls (endo-β-1,3-glucanase, chitinase, and cellulase), exoglycosidases (especially β-glucosidase), proteases, and phosphatases. The most different strain was identified as a not yet described Pythium species. The changes in metabolism of Brassica napus plants grown from seeds coated with the tested Pythium spp. were characterized. Enhanced levels of jasmonates, ethylene precursor, and salicylic acid may indicate better resistance to a wide variety of pathogens. Glucosinolates, as defense compounds against insects and herbivores, were enhanced in young plants. Altogether, P. oligandrum strains varied in their life strategies, and either they could perform equally as plant growth promoters and mycoparasites or they had developed one of these strategies better.
Exploring Beneficial Properties of the Bacteriocinogenic Enterococcus faecium ST10Bz Strain Isolated from Boza, a Bulgarian Cereal-Based Beverage
Authors: Valledor Samantha Joy D. , Bucheli Jorge Enrique Vazquez , Holzapfel Wilhelm H. , Todorov Svetoslav Dimitrov
Published: September 2020

The bacteriocin-producing strain Enterococcus faecium ST10Bz, isolated from boza, a Bulgarian cereal-based beverage, exhibited strong activity against Listeria strains, vancomycin-resistant and other Enterococcus strains, but not against most of the other lactic acid bacteria (LAB) strains included in the test panel. Bacteriocin ST10Bz was proven as a stable antimicrobial, even after exposure to various environmental conditions, including varying pH values, temperatures, and commonly used chemicals in industry and laboratory practice. Bacteriocin activity against L. monocytogenes ATCC®15313™ was recorded at 25,600 AU/mL when the producer strain was cultured in MRS broth at 25 °C and 30 °C, and 19,200 AU/mL, when cultured at 37 °C. Additionally, bacteriocin ST10Bz exhibited bactericidal mode of action when added to actively growing cultures of L. monocytogenes ATCC®15313™ and Enterococcus faecalis 200A. E. faecium ST10Bz was susceptible to the antibiotics kanamycin, gentamycin, ampicillin, streptomycin, tylosin, chloramphenicol, clindamycin, tetracycline, and vancomycin; with no evidence for vanA, B, C, D, E, or G genes. PCR analysis of DNA from strain ST10Bz generated positive results for presence of some bacterial adhesion genes, including map, mub and ef-tu, as well as the gamma aminobutyric acid (GABA) production-related gene, gad. Under simulated gastrointestinal conditions in single and co-culture with L. monocytogenes ATCC®15313™ and E. faecalis 200A, E. faecium ST10Bz showed a high survival rate and the ability to reduce the viable numbers of the two test strains.
Evaluation of Efficiencies of Locally Available Neutralizing Agents for Passive Treatment of Acid Mine Drainage
Authors: Turingan Casey Oliver A. , Singson Giulio B. , Melchor Bernadette T. , Alorro Richard D. , Beltran Arnel B. , Orbecido Aileen H.
Published: September 2020

Acid mine drainage (AMD) generated from the mining industry elevates environmental concerns due to the pollution and contamination it causes to bodies of water. Over the years, passive treatment of AMD using alkalinity-generating materials have been widely studied with pH neutralization as its commonly observed mechanism. During the treatment process, heavy metal removal is also promoted by precipitation due to pH change or through adsorption facilitated by the mineral component of the materials. In this study, four materials were used and investigated: (1) a low grade ore (LGO) made up of goethite, calcium oxide, and manganese aluminum oxide (2–3) limestone and concrete aggregates (CA) composed of calcite, and (4) fly ash consisting of quartz, hematite, and magnetite. The performance of each alkalinity-generating agent at varying AMD/media ratios was based on the change in pH, total dissolved solids (TDS), oxidation reduction potential (EH); and heavy metals (Fe, Ni, and Al) removal and sulfate concentration reduction. Concrete aggregate displayed the most significant effect in treating AMD after raising the pH to 12.42 and removing 99% Fe, 99% Ni, 96% Al, and 57% sulfates. Afterwards, the efficiency of CA at various particle sizes were evaluated over 1 h. The smallest range at 2.00–3.35mm was observed to be most effective after 60 min, raising the pH to 6.78 and reducing 94% Fe, 78% Ni, and 92% Al, but only 28% sulfates. Larger particles of CA were able to remove higher amounts of sulfate up to 57%, similar to the jar test. Overall, CA is an effective treatment media for neutralization; however, its performance can be complemented by a second media for heavy metal and sulfate removal.