bluuebunny/crossref_metadata_2025 huggingface dataset:
Clarification of the Mechanism of Pulse Laser Grinding of Nanosecond Lasers Using High-Speed Camera Imaging
Authors: Liu Xiaoxu , Ni Xianlong , Konda Osamu , Furuhashi Hiroko , Maegawa Satoru , Itoigawa Fumihiro
Published: March 2022

Pulse laser grinding (PLG), as a cutting tool processing method, can not only achieve edge sharpening with high precision, but it can also produce surface modification. For example, polycrystalline cubic boron nitride (PCBN) tools processed by PLG can show increased hardness due to the reduction in defects. However, the mechanism of edge formation under PLG processing remains unclear. In this study, by observing the plasma generated during processing using a high-speed camera, the elementary process for each laser pulse of the PLG process was visualized. The plasma luminescence moved successively through four stages: multipoint luminescence, uniform luminescence, the downward movement of the luminous center, and faint luminescence. By comparing the results of three different laser pulse pitches (0.2, 2, and 20 μm), it was found that the pulse pitch had a significant influence on the PLG processing mode. When the pulse pitch was too small, the sidewall effect was likely to lead to local excess machining. The large pulse pitch resulted in processed surfaces that could not be fully covered by laser irradiation, and it was preferred to remove the decrease threshold subsequently. Thus, the moderate pulse pitch condition showed a superior processed surface compared to the others.
Multi-Objective Optimal Design and Development of a Four-Bar Mechanism for Weed Control
Authors: Hosseini Hooman , Farzad Abdulali , Majeed Faizan , Hensel Oliver , Nasirahmadi Abozar
Published: March 2022

Weeds compete with crops for water, nutrients, and light consequently, have adverse effects on the crop yield and overall productivity. Mechanical weeding is the most common non-chemical method for weed control, which is applied in organic farming, and the weed cultivator is the most common implement in mechanical weeding. This study aimed to design and develop an innovative active tool to optimize the cultivation depth, which can avoid damage to crop roots and improve the key performance indicators of an inter-row cultivator. A quasi-Newton optimization method and a hybrid of the non-dominated sorting genetic algorithm (NSGA-II) and goal attainment method were separately applied to synthesize and develop a four-bar mechanism for weeding requirements. The transmission angle of the mechanism and the desired path of the weeding blade were simultaneously optimized using these multi-objective optimization techniques. The performance of the developed four-bar cultivator based on the optimization techniques was compared with the ones developed based on the classic methods and also with several conventional tools evaluated in other studies. The results showed that applying the quasi-Newton optimization method and hybrid genetic algorithm can propose a more effective weed cultivator in terms of performance indicators, namely weeding performance, mechanical damage to crop plants and cultivation depth. In addition, the optimization of the transmission angle guaranteed the smooth rotations in the mechanism’s joints.
Evaluation of Liner Cavitation Potential through Piston Slap and BEM Acoustics Coupled Analysis
Authors: Wang Xiaoyu , Wang Haofeng , Zhao Jingchao , Zhou Shenghao , Luo Zhong , Han Qingkai
Published: March 2022

Internal combustion engines take up the major position in the power facility market and still encounter some challenges; one key issue is liner cavitation erosion. The impact vibration between piston and cylinder generates pressure fluctuation on the wet liner surface and leads to the occurrence of cavitation in the case that coolant pressure falls below its vapor pressure. Piston slap methodology has been improved by considering the dynamic characteristics of the piston. Water coolant passage acoustic features were investigated and the Helmholtz effect between cylinders was confirmed. In order to address the cavitation erosion potential of the engine cylinder, acoustic pressure in the cooling water passage was investigated by boundary element method analysis with the acceleration of the cylinder liner which was obtained from the piston slap program. This study revealed that a certain acoustic mode of the cooling water passage had a dominant effect on the amplitude of water coolant dynamic pressure induced by liner vibration. Measures of eliminating the acoustic mode are believed to be able to suspend pressure fluctuation and furthermore the potential of cavitation.
An Eagle Strategy Arithmetic Optimization Algorithm for Frequency Stability Enhancement Considering High Renewable Power Penetration and Time-Varying Load
Authors: Elkasem Ahmed. H. A. , Kamel Salah , Hassan Mohamed H. , Khamies Mohamed , Ahmed Emad M.
Published: March 2022

This study proposes a new optimization technique, known as the eagle strategy arithmetic optimization algorithm (ESAOA), to address the limitations of the original algorithm called arithmetic optimization algorithm (AOA). ESAOA is suggested to enhance the implementation of the original AOA. It includes an eagle strategy to avoid premature convergence and increase the populations’ efficacy to reach the optimum solution. The improved algorithm is utilized to fine-tune the parameters of the fractional-order proportional-integral-derivative (FOPID) and the PID controllers for supporting the frequency stability of a hybrid two-area multi-sources power system. Here, each area composites a combination of conventional power plants (i.e., thermal-hydro-gas) and renewable energy sources (i.e., wind farm and solar farm). Furthermore, the superiority of the proposed algorithm has been validated based on 23 benchmark functions. Then, the superiority of the proposed FOPID-based ESAOA algorithm is verified through a comparison of its performance with other controller performances (i.e., PID-based AOA, PID-based ESAOA, and PID-based teaching learning-based optimization TLBO) under different operating conditions. Furthermore, the system nonlinearities, system uncertainties, high renewable power penetration, and control time delay has been considered to ensure the effectiveness of the proposed FOPID based on the ES-AOA algorithm. All simulation results elucidate that the domination in favor of the proposed FOPID-based ES-AOA algorithm in enhancing the frequency stability effectually will guarantee a reliable performance.
Differential Neural Network-Based Nonparametric Identification of Eye Response to Enforced Head Motion
Authors: Chairez Isaac , Mukhamedov Arthur , Prud Vladislav , Andrianova Olga , Chertopolokhov Viktor
Published: March 2022

Dynamic motion simulators cannot provide the same stimulation of sensory systems as real motion. Hence, only a subset of human senses should be targeted. For simulators providing vestibular stimulus, an automatic bodily function of vestibular–ocular reflex (VOR) can objectively measure how accurate motion simulation is. This requires a model of ocular response to enforced accelerations, an attempt to create which is shown in this paper. The proposed model corresponds to a single-layer spiking differential neural network with its activation functions are based on the dynamic Izhikevich model of neuron dynamics. An experiment is proposed to collect training data corresponding to controlled accelerated motions that produce VOR, measured using an eye-tracking system. The effectiveness of the proposed identification is demonstrated by comparing its performance with a traditional sigmoidal identifier. The proposed model based on dynamic representations of activation functions produces a more accurate approximation of foveal motion as the estimation of mean square error confirms.
Subgroup Identification and Regression Analysis of Clustered and Heterogeneous Interval-Censored Data
Authors: Huang Xifen , Xu Jinfeng
Published: March 2022

Clustered and heterogeneous interval-censored data occur in many fields such as medical studies. For example, in a migraine study with the Netherlands Twin Registry, the information including time to diagnosis of migraine and gender was collected for 3975 monozygotic and dizygotic twins. Since each study subject is observed only at discrete and periodic follow-up time points, the failure times of interest (i.e., the time when the individual first had a migraine) are known only to belong to certain intervals and hence are interval-censored. Furthermore, these twins come from different genetic backgrounds and may be associated with differential risks for developing migraines. For simultaneous subgroup identification and regression analysis of such data, we propose a latent Cox model where the number of subgroups is not assumed a priori but rather data-driven estimated. The nonparametric maximum likelihood method and an EM algorithm with monotone ascent property are also developed for estimating the model parameters. Simulation studies are conducted to assess the finite sample performance of the proposed estimation procedure. We further illustrate the proposed methodologies by an empirical analysis of migraine data.
A Note on Exponential Stability for Numerical Solution of Neutral Stochastic Functional Differential Equations
Authors: Wang Qi , Chen Huabin , Yuan Chenggui
Published: March 2022

This paper examines the numerical solutions of the neutral stochastic functional differential equation. This study establishes the discrete stochastic Razumikhin-type theorem to investigate the exponential stability in the mean square sense of the Euler–Maruyama numerical solution to this equation. In addition, the Borel–Cantelli lemma and the stochastic analysis theory are incorporated to discuss the almost sure exponential stability for this numerical solution of such equations.
New Modular Fixed-Point Theorem in the Variable Exponent Spaces ℓp(.)
Authors: El Amri Amnay , Khamsi Mohamed A.
Published: March 2022

In this work, we prove a fixed-point theorem in the variable exponent spaces ℓp(.), when p−=1 without further conditions. This result is new and adds more information regarding the modular structure of these spaces. To be more precise, our result concerns ρ-nonexpansive mappings defined on convex subsets of ℓp(.) that satisfy a specific condition which we call “condition of uniform decrease”.
On a New Family of Runge–Kutta–Nyström Pairs of Orders 6(4)
Authors: Kovalnogov Vladislav N. , Fedorov Ruslan V. , Generalov Dmitry A. , Tsvetova Ekaterina V. , Simos Theodore E. , Tsitouras Charalampos
Published: March 2022

In this study, Runge–Kutta–Nyström pairs of orders 6(4) using six stages per step are considered. The main contribution of the present work is that we introduce a new family of pairs (i.e., new methodology of solution for order conditions) that possesses seven free parameters instead of four, as used by similar pairs until now. Using these extra coefficients efficiently we may construct methods with better properties. Here, we exploit the free parameters in order to derive a pair with extended imaginary stability interval. This type of method may furnish better results on problems with periodic solutions. Extended numerical tests justify our effort.
Multiple Benefit Thresholds Problem in Online Social Networks: An Algorithmic Approach
Authors: Pham Phuong N. H. , Nguyen Bich-Ngan T. , Co Quy T. N. , Snášel Václav
Published: March 2022

An important problem in the context of viral marketing in social networks is the Influence Threshold (IT) problem, which aims at finding some users (referred to as a seed set) to begin the process of disseminating their product’s information so that the benefit gained exceeds a predetermined threshold. Even though, marketing strategies exhibit different in several realistic scenarios due to market dependence or budget constraints. As a consequence, picking a seed set for a specific threshold is not enough to come up with an effective solution. To address the disadvantages of previous works with a new approach, we study the Multiple Benefit Thresholds (MBT), a generalized version of the IT problem, as a result of this phenomenon. Given a social network that is subjected to information distribution and a set of thresholds, T={T1,T2,…,Tk},Ti>0, the issue aims to seek the seed sets S1,S2,…,Sk with the lowest possible cost so that the benefit achieved from the influence process is at the very least T1,T2,…,Tk, respectively. The main challenges of this problem are a #NP-hard problem and the estimation of the objective function #P-Hard under traditional information propagation models. In addition, adapting the exist algorithms many times to different thresholds can lead to large computational costs. To address the abovementioned challenges, we introduced Efficient Sampling for Selecting Multiple Seed Sets, an efficient technique with theoretical guarantees (ESSM). At the core of our algorithm, we developed a novel algorithmic framework that (1) can use the solution to a smaller threshold to find that of larger ones and (2) can leverage existing samples with the current solution to find that of larger ones. The extensive experiments on several real social networks were conducted in order to show the effectiveness and performance of our algorithm compared with current ones. The results indicated that our algorithm outperformed other state-of-the-art ones in terms of both the total cost and running time.
Effect of Dope Flow Rate and Post-Treatment on the Morphology, Permeation and Metal Ion Rejection from PES/LiBr-Based UF Hollow Fiber Membranes
Authors: Irfan Muhammad , Irfan Masooma , Idris Ani , Alsubaie Abdullah Saad , Mahmoud Khaled H. , Mohd Yusof Noordin , Muhammad Nawshad
Published: March 2022

This study investigated the influence of dope extrusion rate (DER) and post-treatment effect on the morphology, permeation, and metal ion rejection by polyethersulfone/lithium bromide (PES/LiBr)-based hollow fiber (HF) membranes. HF fibers were spun with 2.25, 2.5, and 3.1 ratios of DER to bore fluid rate (BFR), wherein DER varied from 11.35, 12.5, to 15.6 mL/min with a fixed BFR (5 mL/min). Molecular weight cutoff (MWCO), pore size, water flux, and flux recovery ratio were determined, whereas lake water was used to observe the rejection rate of dissolved metallic ions. Results showed that with the increase of the DER wall thickness (WT), HFs increased from 401.5 to 419.5 um, and furthermore by the post-treatments up to 548.2 um, as confirmed by field emission scanning electron microscope (FESEM) analysis. Moreover, MWCO, pore size, and the pure water permeation (PWP) of the HF membranes decreased, while the separation performance for polyethylene glycol (PEG) solute increased with increasing DER. Post-treated HFs from 11.35 mL/min of DER showed 93.8% of MWCO value with up to 90% and 70% rejection of the arsenic and chromium metallic ions, respectively, in comparison with all other formulated HFs.
Behavior of As/AsxSy in Neutral and Oxidizing Atmospheres at High Temperatures—An Overview
Authors: Castro Kristhobal , Balladares Eduardo , Jerez Oscar , Pérez-Tello Manuel , Aracena Álvaro
Published: March 2022

The reaction mechanisms in As and As-S systems during their oxidation and/or thermal decomposition are complex to describe due to the physicochemical characteristics of arsenic and its sulfides; the information highlighted in the literature was analyzed and correlated to determinate the predominant phases and reaction mechanisms during the thermal decomposition and oxidation of arsenic, in its elemental form and in sulfurate phases. As a result of this analysis, it was determined that the predominant phases are mainly composed of allotropies of arsenic, sulfides, and dimers. In addition, reaction mechanisms are provided that describe the behavior of arsenic and its sulfides during its thermal decomposition and oxidation.
Research on the Negative Multistage Incremental Forming of Straight-Wall Parts Based on the Extrusion from the Forward and Reverse Side of the Sheet
Authors: Cheng Guixi , Zhu Hu , Jung Dongwon
Published: March 2022

Because the forming area involved in traditional reverse multistage incremental forming is only located inside the model, the sheet-metal thinning rate is relatively large. Particularly, the straight-wall parts with a narrow internal space cannot be formed using traditional multistage incremental forming. Therefore, a negative multistage incremental forming that extrudes the sheet from the forward and the reverse side of the straight-wall part is proposed in this paper. In this method, firstly, the auxiliary surface is generated to divide the straight-wall part model into three forming regions; secondly, the first- and second-stage forming are carried out from the forward side of the straight-wall part with support, respectively; Thirdly, the third-stage forming is carried out from the forward side of the straight-wall part without support. The software system for auxiliary-surface generation, the straight-wall parts partition, each intermediate-stage-forming model, and each stage-forming toolpath generation was developed by using C++, VC++, and OpenGL library. In order to verify the feasibility of the proposed method in this paper, the forming experiments of a 1060 aluminum sheet were conducted using traditional reverse multistage forming and the proposed method in this paper, and the forming effects were compared and analyzed. The results show that compared with traditional reverse multistage incremental forming with forward-side extrusion, the multistage incremental forming method with the forward and the reverse-sided extrusion proposed in this paper can increase the area of the sheet participating in the deformation and avoid the problem of excessive thinning of sheet thickness, especially suitable for the straight-wall part model with narrow internal space.
Mechanistic Insights of Mitochondrial Dysfunction in Amyotrophic Lateral Sclerosis: An Update on a Lasting Relationship
Authors: Candelise Niccolò , Salvatori Illari , Scaricamazza Silvia , Nesci Valentina , Zenuni Henri , Ferri Alberto , Valle Cristiana
Published: March 2022

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive loss of the upper and lower motor neurons. Despite the increasing effort in understanding the etiopathology of ALS, it still remains an obscure disease, and no therapies are currently available to halt its progression. Following the discovery of the first gene associated with familial forms of ALS, Cu–Zn superoxide dismutase, it appeared evident that mitochondria were key elements in the onset of the pathology. However, as more and more ALS-related genes were discovered, the attention shifted from mitochondria impairment to other biological functions such as protein aggregation and RNA metabolism. In recent years, mitochondria have again earned central, mechanistic roles in the pathology, due to accumulating evidence of their derangement in ALS animal models and patients, often resulting in the dysregulation of the energetic metabolism. In this review, we first provide an update of the last lustrum on the molecular mechanisms by which the most well-known ALS-related proteins affect mitochondrial functions and cellular bioenergetics. Next, we focus on evidence gathered from human specimens and advance the concept of a cellular-specific mitochondrial “metabolic threshold”, which may appear pivotal in ALS pathogenesis.
Nonribosomal Peptide Synthesis Definitely Working Out of the Rules
Authors: Duban Matthieu , Cociancich Stéphane , Leclère Valérie
Published: March 2022

Nonribosomal peptides are microbial secondary metabolites exhibiting a tremendous structural diversity and a broad range of biological activities useful in the medical and agro-ecological fields. They are built up by huge multimodular enzymes called nonribosomal peptide synthetases. These synthetases are organized in modules constituted of adenylation, thiolation, and condensation core domains. As such, each module governs, according to the collinearity rule, the incorporation of a monomer within the growing peptide. The release of the peptide from the assembly chain is finally performed by a terminal core thioesterase domain. Secondary domains with modifying catalytic activities such as epimerization or methylation are sometimes included in the assembly lines as supplementary domains. This assembly line structure is analyzed by bioinformatics tools to predict the sequence and structure of the final peptides according to the sequence of the corresponding synthetases. However, a constantly expanding literature unravels new examples of nonribosomal synthetases exhibiting very rare domains and noncanonical organizations of domains and modules, leading to several amazing strategies developed by microorganisms to synthesize nonribosomal peptides. In this review, through several examples, we aim at highlighting these noncanonical pathways in order for the readers to perceive their complexity.
Incorporation of Plant Extracted Hydroxyapatite and Chitosan Nanoparticles on the Surface of Orthodontic Micro-Implants: An In-Vitro Antibacterial Study
Authors: Alhazmi Anwar S. , Syame Sohier M. , Mohamed Wael S. , Hakim Ashraf S.
Published: March 2022

In our study, the structural and morphological applications of hydroxyapatite and chitosan nanoparticles and coated micro-implants were assessed for their ability to combat oral pathogenic bacteria. The hydroxyapatite, as well as chitosan nanoparticles, were synthesized from the Salvadora persica plant. The crystal morphology, phase composition, particle size, and surface functional groups of the nano-samples were analyzed via classical examinations and energy dispersive X-ray analysis. The prepared nanoparticles have been examined for antibacterial activity against four common oral bacterial strains. The antimicrobial effect was also assessed by the Live/Dead BacLight technique in combination with confocal scanning laser microscopy. Titanium micro-implants were coated with regular hydroxyapatite (HAP) and chitosan nanoparticles, and the surface was characterized by scanning electron microscopy. The analysis asserted elemental composition of the prepared nanoparticles and their textural features, metal crystallization, and functional bonds. The antibacterial activity of the nanoparticles was evaluated against oral pathogenic microorganisms by the disc diffusion method, minimum bacterial concentration (MBC), and minimum inhibitory concentration (MIC). Chitosan nanoparticles showed (MICs) of 8 μg mL−1 for (Streptococcus salivarius, Streptococcus mutans and Enterococcus faecalis), and 16 μg mL−1 for Streptococcus sanguinis. HAP nanoparticles showed (MICs) of 16 μg/mL for E. faecalis, and S. sanguis, 8 μg/mL for S. salivarius and finally 4 μg/mL for S. mutans. HAP nanoparticles showed enhanced antibacterial activity and more obvious damage in the bacterial cell membrane than that of synthesized chitosan nanoparticles. The prepared nanoparticles could successfully coat titanium microplates to enhance their efficiency.
Bacteriocin Producing Streptococcus agalactiae Strains Isolated from Bovine Mastitis in Brazil
Authors: Vidal Amaral João Ricardo , Jucá Ramos Rommel Thiago , Almeida Araújo Fabrício , Bentes Kato Rodrigo , Figueira Aburjaile Flávia , de Castro Soares Siomar , Góes-Neto Aristóteles , Matiuzzi da Costa Mateus , Azevedo Vasco , Brenig Bertram , Soares de Oliveira Selma , Soares Rosado Alexandre
Published: March 2022

Antibiotic resistance is one of the biggest health challenges of our time. We are now facing a post-antibiotic era in which microbial infections, currently treatable, could become fatal. In this scenario, antimicrobial peptides such as bacteriocins represent an alternative solution to traditional antibiotics because they are produced by many organisms and can inhibit bacteria, fungi, and/or viruses. Herein, we assessed the antimicrobial activity and biotechnological potential of 54 Streptococcus agalactiae strains isolated from bovine mastitis. Deferred plate antagonism assays revealed an inhibition spectrum focused on species of the genus Streptococcus—namely, S. pyogenes, S. agalactiae, S. porcinus, and S. uberis. Three genomes were successfully sequenced, allowing for their taxonomic confirmation via a multilocus sequence analysis (MLSA). Virulence potential and antibiotic resistance assessments showed that strain LGMAI_St_08 is slightly more pathogenic than the others. Moreover, the mreA gene was identified in the three strains. This gene is associated with resistance against erythromycin, azithromycin, and spiramycin. Assessments for secondary metabolites and antimicrobial peptides detected the bacteriocin zoocin A. Finally, comparative genomics evidenced high similarity among the genomes, with more significant similarity between the LGMAI_St_11 and LGMAI_St_14 strains. Thus, the current study shows promising antimicrobial and biotechnological potential for the Streptococcus agalactiae strains.
Isolation, Characterization, and Genomic Analysis of Three Novel E. coli Bacteriophages That Effectively Infect E. coli O18
Authors: Abdelrahman Fatma , Rezk Nouran , Fayez Mohamed S. , Abdelmoteleb Mohamed , Atteya Reham , Elhadidy Mohamed , El-Shibiny Ayman
Published: March 2022

Escherichia coli (E. coli) is one of the most common pathogenic bacteria worldwide. Avian pathogenic E. coli (APEC) causes severe systemic disease in poultry (Colibacillosis), and accordingly, has an extreme risk to the poultry industry and public health worldwide. Due to the increased rate of multi-drug resistance among these bacteria, it is necessary to find an alternative therapy to antibiotics to treat such infections. Bacteriophages are considered one of the best solutions. This study aimed to isolate, characterize, and evaluate the potential use of isolated bacteriophages to control E. coli infections in poultry. Three novel phages against E. coli O18 were isolated from sewage water and characterized in vitro. The genome size of the three phages was estimated to be 44,776 bp, and the electron microscopic analysis showed that they belonged to the Siphoviridae family, in the order Caudovirales. Phages showed good tolerance to a broad range of pH and temperature. The complete genomes of three phages were sequenced and deposited into the GenBank database. The closely related published genomes of Escherichia phages were identified using BLASTn alignment and phylogenetic trees. The prediction of the open reading frames (ORFs) identified protein-coding genes that are responsible for functions that have been assigned such as cell lysis proteins, DNA packaging proteins, structural proteins, and DNA replication/transcription/repair proteins.
Hemorrhagic Fever Viruses: Pathogenesis and Countermeasures
Authors: Mire Chad E. , Marzi Andrea
Published: March 2022

Before December 2019 and the COVID-19 pandemic, the general public was to some extent aware that zoonotic viruses can spill over into the human population and cause a disease outbreak [...]
Epidemiology of Echovirus 30 Infections Detected in a University Hospital in Catalonia, Spain, in 1995–2020
Authors: del Cuerpo Margarita , Gonzalez de Audicana Jon , Fernandez-Garcia Maria Dolores , Marín Pilar , Esteban Montserrat , Español Montserrat , Cabrerizo María , Rabella Núria
Published: March 2022

There is a growing interest in echovirus 30 (E30), an enterovirus responsible for neurological disease and hospitalization. There are multiple studies of outbreaks, but few that study the epidemiology over long periods of time. Our study aims to describe the clinical, epidemiological and microbiological characteristics of a series of E30 infections detected over 26 years. Data were retrospectively collected from a database of all enterovirus infections identified in our laboratory. They were detected by viral isolation or nucleic acid detection in patients presenting with respiratory or neurological infections, rash, sepsis-like syndrome, or gastroenteritis. Enterovirus genotyping was performed by amplification of the VP1 gene using RT-nested PCR, followed by sequencing and BLAST analysis. Of the 2402 enterovirus infections detected, 1619 were linked to at least one genotype and 173 were caused by E30. Clinical information was available for 158 (91.3%) patients. E30 was associated with neurological infection in 107 (67.8%) cases and it was detected almost every year. Phylogenetic analysis was performed with 67 sequences. We observed that E30 strains circulating in Catalonia from 1996 to 2016 belong to two lineages (E and F), although the majority cluster was in F. In 2018, lineage I emerged as the dominant lineage.
Genetic and Antigenic Characteristics of Highly Pathogenic Avian Influenza A(H5N8) Viruses Circulating in Domestic Poultry in Egypt, 2017–2021
Authors: Kandeil Ahmed , Moatasim Yassmin , El Taweel Ahmed , El Sayes Mohamed , Rubrum Adam , Jeevan Trushar , McKenzie Pamela P. , Webby Richard J. , Ali Mohamed A. , Kayali Ghazi , El-Shesheny Rabeh
Published: March 2022

In Egypt, the endemicity of avian influenza viruses is a serious concern. Since 2016, several outbreaks of H5N8 have been recorded among domestic poultry in various areas of the country. Active surveillance of domestic poultry across several governorates in Egypt from 2017 to 2021 detected at least six genotypes of Highly Pathogenic Avian Influenza (HPAI) H5N8 viruses with evidence of partial or complete annual replacement of dominant strains. Although all Egyptian H5N8 viruses had clade 2.3.4.4b hemagglutinin (HA) genes, the remaining viral gene segments were from multiple geographic origins, indicating that the H5N8 isolates resulted from multiple introductions. Mutations in the viral proteins associated with pathogenicity and antiviral drug resistance were detected. Some mutations in the HA resulted in antigenic drift. Heterogeneity in circulating H5N8 HPAI threatens poultry production and public health.
Insights into the Antibacterial Activity of Prolactin-Inducible Protein against the Standard and Environmental MDR Bacterial Strains
Authors: Yousuf Mohd , Ali Asghar , Khan Parvez , Anjum Farah , Elasbali Abdelbaset Mohamed , Islam Asimul , Yadav Dharmendra Kumar , Shafie Alaa , Rizwanul Haque Qazi Mohd. , Hassan Md. Imtaiyaz
Published: March 2022

Background: Prolactin inducible protein (PIP) is a small secretary glycoprotein present in most biological fluids and contributes to various cellular functions, including cell growth, fertility, antitumor, and antifungal activities. Objectives: The present study evaluated the antibacterial activities of recombinant PIP against multiple broad-spectrum MDR bacterial strains. Methods: The PIP gene was cloned, expressed and purified using affinity chromatography. Disk diffusion, broth microdilution, and growth kinetic assays were used to determine the antibacterial activities of PIP. Results: Disk diffusion assay showed that PIP has a minimum and maximum zone of inhibition against E. coli and P. aeruginosa, respectively, compared to the reference drug ampicillin. Furthermore, growth kinetics studies also suggested that PIP significantly inhibited the growth of E. coli and P. aeruginosa. The minimum inhibitory concentration of PIP was 32 µg/mL for E. coli (443), a standard bacterial strain, and 64 µg/mL for Bacillus sp. (LG1), an environmental multidrug-resistant (MDR) strain. The synergistic studies of PIP with ampicillin showed better efficacies towards selected bacterial strains having MDR properties. Conclusion: Our findings suggest that PIP has a broad range of antibacterial activities with important implications in alleviating MDR problems.
The Role of Scandium Substitution in Babingtonite Group Minerals
Authors: Nagashima Mariko , Nishio-Hamane Daisuke , Matsumoto Takashi , Fukuda Chihiro
Published: March 2022

Sc-rich babingtonite from Heftetjern, Norway and Baveno, Italy were examined using electron microprobe analysis and X-ray single-crystal refinement in order to re-examine the behavior of Sc3+ and analyze its effect on the crystal structure of babingtonite. The Sc2O3 content is 13.78 wt.% in the Heftetjern specimen, and 8.44 wt.% in the Baveno one. In contrast, the latter has higher Fe content (11.13 wt.% as FeO) rather than the former one (8.63 wt.% as FeO). Characteristically, both specimens contain sodium. Although the oxidation state of octahedral cations in babingtonites is in general Me2+:Me3+ = 1:1, trivalent cations in the Heftetjern specimen attain 1.14 apfu. This excess of trivalent cations must be counterbalanced by monovalent Na substituted for Ca. The unit-cell parameters are a = 7.5272(1), b = 11.7175(1), c = 6.7613(1) Å, α = 91.710(1), β = 93.637(1), γ = 104.522(1)°, and V = 575.49(2) Å3 for the Heftetjern specimen, and a = 7.5199(2), b = 11.7145(3), c = 6.7408(2) Å, α = 91.756(2), β = 93.786(2), γ = 104.549(2)°, and V = 573.83(3) Å3 for the Baveno one. The structural formulae are A1Ca1.00A2(Ca0.879Na0.121)M1(Sc3+0.42Fe2+0.37Mn2+0.21)M2(Sc3+0.68Fe2+0.27Mg0.03Fe3+0.02)Si5O14(OH) for Heftetjern, and A1Ca1.00A2(Ca0.819Na0.181)M1(Sc3+0.43Mn2+0.36Fe2+0.21)M2(Fe3+0.36Fe2+0.30Sc3+0.26Sn4+0.05Al0.03)Si5O14(OH) for Baveno. Due to Sc3+ substitution, the  distance, 2.09–2.11 Å, is longer than that of Sc-free babingtonite, 2.03–2.05 Å. The M2O6 expansion leads to the lengthened O4–O10 edge shared between the M1O6 and M2O6 octahedra, and causes the stronger angular distortion of M2O6. This can be explained by the increase of the O4–M1–O10 angle and decrease of the O4–M1–O8 angle with lengthening of the O4–O10 edge.
High-Throughput Analysis of Offshore Well Drill-Cuttings via SEM-Automated Mineralogy Using Single-Step Trans-Vertical Moulds
Authors: Grant David , Goudie Dylan J. , Wilton Derek
Published: March 2022

Single-step trans-vertical moulds used to prepare grain mounts for SEM-automated-mineralogy based instruments have been shown to lead to more representative sample statistics. For samples that contain a variety of mineral phases, denser phases can settle to the bottom of an epoxy mould during the curing phase. Slicing and polishing a cross section through density-stratified moulds of the sample material can compensate for this. Aliquots from offshore drill well cuttings were prepared using three different methods: (1) traditional epoxy mounting, (2) two-step trans-vertical moulds, and (3) single-step trans-vertical moulds. All were analysed with a FEI Quanta 650 FEG instrument using MLA software with similar acquisition parameters. The results indicate that the single-step trans-vertical moulds are reproducible, lead to more accurate statistics, and yield MLA calculated elemental assays that closely match with data from standard analytical methods. In addition, these moulds can be made in half the time of the two-step trans-vertical moulds, and because of the unique geometry of the custom-made SEM holders, the analytical throughput is doubled. Depending on the size fraction of the well cuttings, the throughput can be further increased by slicing off the remaining epoxy to allow for more samples in the 26x TV holder. A unique cell holder was created to accommodate these trans-vertical samples, allowing them to fit into a LA-ICP-MS instrument such that detailed follow-up microanalyses can be conducted on specific minerals. For instance geochronology can be conducted on grains of interest (e.g., zircons), which had been mapped by the SEM-MLA.
Isotope Geochemistry of the Shenshuitan Gold Deposit within the Wulonggou Gold Field in the Eastern Kunlun Orogen, Northwest China: Implications for Metallogeny
Authors: Zhou Xuan , Pan Tong , Ding Qing-Feng , Cheng Long , Song Kai , Liu Fei , Gao Yang
Published: March 2022

The Shenshuitan gold deposit is located within the Eastern Kunlun Orogen in northwest China. The gold mineralization here occurs primarily within the ductile fault XI. The sulfide mineral assemblage is dominated by pyrite and arsenopyrite, with minor pyrrhotite, chalcopyrite, galena, and sphalerite. Host rocks predominantly consist of Ordovician silicic slate and late Silurian granites, and their alterations include silicification and sericitization. The measured δD and δ18O values of quartz and sericite range from −113.9‰ to −93.1‰ and from 4.6‰ to 12.0‰, respectively. Bulk and in situ δ34S values of sulfides range from −7.3‰ to +9.6‰ and from −3.92‰ to 11.04‰, respectively. Lead isotope compositions of sulfides show 206Pb/204Pb ratios from 18.071 to 19.341, 207Pb/204Pb ratios from 15.530 to 15.67, and 208Pb/204Pb ratios from 37.908 to 38.702. Collectively, the isotope (H, O, S, and Pb) geochemistry suggests that the ore-forming fluids were of a metamorphic origin mixed with meteoric water and that the sulfur and lead were sourced from a mixture of host rocks and original ore-forming metamorphic fluids. Lastly, this deposit can be classified as an orogenic gold deposit associated with the final collision between the Bayan Har–Songpanganzi Terrane and the Eastern Kunlun Orogen during the Later Triassic.
Evaluating the Co-design of an Age-Friendly, Rural, Multidisciplinary Primary Care Model: A Study Protocol
Authors: Winterton Rachel , Brasher Kathleen , Ashcroft Mark
Published: March 2022

In the context of increased rates of frailty and chronic disease among older people, there is a need to develop age-friendly, integrated primary care models that place the older person at the centre of their care. However, there is little evidence about how age-friendly integrated care frameworks that are sensitive to the challenges of rural regions can be developed. This protocol paper outlines a study that will examine how the use of an age-friendly care framework (the Indigo 4Ms Framework) within a co-design process can facilitate the development of models of integrated care for rural older people within the Upper Hume region (Victoria, Australia). A co-design team will be assembled, which will include older people and individuals from local health, aged care, and community organisations. Process and outcome evaluation of the co-design activities will be undertaken to determine (1) the processes, activities and outputs that facilitate or hinder the co-design of a 4Ms integrated approach, and (2) how the use of the Indigo 4Ms Framework within a co-design process contributes to more integrated working practices. This protocol contributes to the development of a field of study examining how rural health and aged care services can become more age-friendly, with an emphasis on the role of co-design in developing integrated approaches to health care for older adults.
The Positive Temperature Coefficient of Resistivity in BiFeO3 Films
Authors: Yang Qianqian , Wang Xiaolei , Yang Kaihua , Deng Jinxiang , Nie Ruijuan , Deng Qingsong , Chen Xuegang , Yang Hongwei , Xu Kailin , Wang Furen
Published: March 2022

The use of lead-free ceramic film materials with positive temperature coefficient of resistivity (PTCR) is widespread in temperature heaters and sensors in micro-electromechanical systems. In this research, the out of plane transport properties of the BiFeO3 (BFO) films have been studied. Surprisingly, PTCR was found in the BFO ceramic films due to the strongly correlated interaction between the multiferroic material BFO and the superconductor YBCO perovskite oxides. To our knowledge, this is the first report on the PTCR effect of BFO films. The BFO/YBCO interface and the bulk conductivity of BFO are important for the PTCR effect, as they make it possible to compare the transport properties of Au/BFO/YBCO- and YBCO/BFO/YBCO-type structures. PTCR was observed in Au/BFO/YBCO at a bias voltage of more than 2 V, but not in the YBCO/BFO/YBCO, even with a 40 V bias voltage. PTCR was found after BFO breakdown of a YBCO/BFO/YBCO capacitor. This indicated that the conductivity of BFO is critical for PTCR. The dependence of PTCR on the superconducting transition temperature illustrates that a cooper-pair can be injected into BFO. Our work presents a method by which to produce a lead-free ceramic film material with PTCR.
Facile Preparation of Mechanically Robust and Functional Silica/Cellulose Nanofiber Gels Reinforced with Soluble Polysaccharides
Authors: Beaumont Marco , Jahn Elisabeth , Mautner Andreas , Veigel Stefan , Böhmdorfer Stefan , Potthast Antje , Gindl-Altmutter Wolfgang , Rosenau Thomas
Published: March 2022

Nanoporous silica gels feature extremely large specific surface areas and high porosities and are ideal candidates for adsorption-related processes, although they are commonly rather fragile. To overcome this obstacle, we developed a novel, completely solvent-free process to prepare mechanically robust CNF-reinforced silica nanocomposites via the incorporation of methylcellulose and starch. Significantly, the addition of starch was very promising and substantially increased the compressive strength while preserving the specific surface area of the gels. Moreover, different silanes were added to the sol/gel process to introduce in situ functionality to the CNF/silica hydrogels. Thereby, CNF/silica hydrogels bearing carboxyl groups and thiol groups were produced and tested as adsorber materials for heavy metals and dyes. The developed solvent-free sol/gel process yielded shapable 3D CNF/silica hydrogels with high mechanical strength; moreover, the introduction of chemical functionalities further widens the application scope of such materials.
Three-Dimensional MoS2/Reduced Graphene Oxide Nanosheets/Graphene Quantum Dots Hybrids for High-Performance Room-Temperature NO2 Gas Sensors
Authors: Yang Cheng , Wang Yanyan , Wu Zhekun , Zhang Zhanbo , Hu Nantao , Peng Changsi
Published: March 2022

This study presents three-dimensional (3D) MoS2/reduced graphene oxide (rGO)/graphene quantum dots (GQDs) hybrids with improved gas sensing performance for NO2 sensors. GQDs were introduced to prevent the agglomeration of nanosheets during mixing of rGO and MoS2. The resultant MoS2/rGO/GQDs hybrids exhibit a well-defined 3D nanostructure, with a firm connection among components. The prepared MoS2/rGO/GQDs-based sensor exhibits a response of 23.2% toward 50 ppm NO2 at room temperature. Furthermore, when exposed to NO2 gas with a concentration as low as 5 ppm, the prepared sensor retains a response of 15.2%. Compared with the MoS2/rGO nanocomposites, the addition of GQDs improves the sensitivity to 21.1% and 23.2% when the sensor is exposed to 30 and 50 ppm NO2 gas, respectively. Additionally, the MoS2/rGO/GQDs-based sensor exhibits outstanding repeatability and gas selectivity. When exposed to certain typical interference gases, the MoS2/rGO/GQDs-based sensor has over 10 times higher sensitivity toward NO2 than the other gases. This study indicates that MoS2/rGO/GQDs hybrids are potential candidates for the development of NO2 sensors with excellent gas sensitivity.
Synthesis and Investigation of Antibacterial Activity of Thin Films Based on TiO2-Ag and SiO2-Ag with Potential Applications in Medical Environment
Authors: Adochițe Cristina-Ș. , Vițelaru Cătălin , Parau Anca C. , Kiss Adrian E. , Pană Iulian , Vlădescu Alina , Costinaș Sarah , Moga Marius , Muntean Radu , Badea Mihaela , Idomir Mihaela
Published: March 2022

Multiple antibiotic resistance has now become a major obstacle to the treatment of infectious diseases. In this context, the application of nanotechnology in medicine is a promising alternative for the prevention of infections with multidrug-resistant germs. The use of silver as a powerful antibacterial agent has attracted much interest. TiO2 and SiO2 thin films enhanced with Ag particles have been developed with the aim of maintaining the transparency of the polymer films. Antibacterial activity was evaluated for a Gram-negative species-Escherichia coli-in concentrations of 105 and 104 CFU/mL in different conditions-activation by UV irradiation, single layer and double layer. Increased antibacterial efficacy of TiO2-deposited foil was found for the tests that had been exposed to UV activation. In the case of bilayer tests, the efficiency was higher compared to those in a single layer, as the contact surface between the films and the bacterial suspension increased. Films can be used as a potential method to limit bacterial growth on hospital surfaces, such as telephone screens and medical equipment, given their optimized characteristics and proven antibacterial efficacy.
Hymenolepis diminuta Infection in a Romanian Child from an Urban Area
Authors: Galoș Felicia , Anghel Mălina , Ioan Andreea , Ieșanu Mara-Ioana , Boboc Cătălin , Boboc Anca Andreea
Published: March 2022

Hymenolepis diminuta is primarily a rodent parasite that is ubiquitously distributed worldwide, but with only a few cases described as human infections. We report a case of Hymenolepis diminuta infection in a 15-month-old child, living in an urban setting, with no previous medical history. The patient presented with two episodes of seizures, and complaints of abdominal pain, vomiting, and diarrhea, with no apparent history of rodent contact. Furthermore, the patient’s gastrointestinal symptoms were linked to the emission of suspected tapeworm proglottids in the feces. After excluding other possible etiologies, a diagnosis of Hymenolepis diminuta infection was made, based on the examination of characteristic eggs in a concentrated stool specimen. The infant was successfully treated with praziquantel and fully recovered. After two weeks, the stool sample was free of Hymenolepis diminuta eggs. The clinical follow-up over the next 3 years was normal. Hymenolepis diminuta is rarely found in humans, and, when present, the infection is frequently asymptomatic. Abdominal pain, irritability, itching, eosinophilia, and seizures have also been reported. In this paper, we report, for the first time in the literature, an infection with Hymenolepis diminuta in a Romanian infant who had atypical neurological presentation, with full recovery, without subsequent neurological sequelae.
A Multi-Laboratory Comparison of Methods for Detection and Quantification of African Swine Fever Virus
Authors: Olesen Ann Sofie , Bruun Rasmussen Thomas , Saxmose Nielsen Søren , Belsham Graham J. , Boklund Anette , Ploegaert Tosca , Moonen-Leusen Bernie , Blome Sandra , Bøtner Anette
Published: March 2022

African swine fever is a viral disease of the family Suidae. Methods to detect and quantify African swine fever virus (ASFV) include qPCR and virus infectivity assays. Individual laboratories often use in-house procedures for these assays, which can hamper the comparison of results. The objective of this study was to estimate the probability of ASFV detection using these assays, and to determine the inter-test correlations between results. This was achieved by testing a panel of 80 samples at three reference laboratories. Samples were analysed using nucleic acid extraction and qPCR, as well as virus infectivity assays. For qPCR, a very high probability (ranging from 0.96 to 1.0) of detecting ASFV DNA was observed for all tested systems. For virus infectivity assays in cells, the probability of detecting infectious ASFV varied from 0.68 to 0.90 and was highest using pulmonary alveolar macrophages, followed by MARC145 cells, peripheral blood monocytes, and finally wild boar lung cells. Intraclass correlation coefficient estimates of 0.97 (0.96–0.98) between qPCR methods, 0.80 (0.74–0.85) to 0.94 (0.92–0.96) between virus infectivity assays, and 0.77 (0.68–0.83) to 0.95 (0.93–0.96) between qPCR methods and virus infectivity assays were obtained. These findings show that qPCR gives the highest probability for the detection of ASFV.