bluuebunny/crossref_metadata_2025 huggingface dataset:
Inhibition of Protein Prenylation of GTPases Alters Endothelial Barrier Function
Authors: Aslam Muhammad , Troidl Christian , Tanislav Christian , Rohrbach Susanne , Gündüz Dursun , Hamm Christian W.
Published: December 2019

The members of Rho family of GTPases, RhoA and Rac1 regulate endothelial cytoskeleton dynamics and hence barrier integrity. The spatial activities of these GTPases are regulated by post-translational prenylation. In the present study, we investigated the effect of prenylation inhibition on the endothelial cytoskeleton and barrier properties. The study was carried out in human umbilical vein endothelial cells (HUVEC) and protein prenylation is manipulated with various pharmacological inhibitors. Inhibition of either complete prenylation using statins or specifically geranylgeranylation but not farnesylation has a biphasic effect on HUVEC cytoskeleton and permeability. Short-term treatment inhibits the spatial activity of RhoA/Rho kinase (Rock) to actin cytoskeleton resulting in adherens junctions (AJ) stabilization and ameliorates thrombin-induced barrier disruption whereas long-term inhibition results in collapse of endothelial cytoskeleton leading to increased basal permeability. These effects are reversed by supplementing the cells with geranylgeranyl but not farnesyl pyrophosphate. Moreover, long-term inhibition of protein prenylation results in basal hyper activation of RhoA/Rock signaling that is antagonized by a specific Rock inhibitor or an activation of cAMP signaling. In conclusion, inhibition of geranylgeranylation in endothelial cells (ECs) exerts biphasic effect on endothelial barrier properties. Short-term inhibition stabilizes AJs and hence barrier function whereas long-term treatment results in disruption of barrier properties.
How the Other Half Lives: What p53 Does When It Is Not Being a Transcription Factor
Authors: Ho Teresa , Tan Ban Xiong , Lane David
Published: December 2019

It has been four decades since the discovery of p53, the designated ‘Guardian of the Genome’. P53 is primarily known as a master transcription factor and critical tumor suppressor, with countless studies detailing the mechanisms by which it regulates a host of gene targets and their consequent signaling pathways. However, transcription-independent functions of p53 also strongly define its tumor-suppressive capabilities and recent findings shed light on the molecular mechanisms hinted at by earlier efforts. This review highlights the transcription-independent mechanisms by which p53 influences the cellular response to genomic instability (in the form of replication stress, centrosome homeostasis, and transposition) and cell death. We also pinpoint areas for further investigation in order to better understand the context dependency of p53 transcription-independent functions and how these are perturbed when TP53 is mutated in human cancer.
Influence of Acrylamide Administration on the Neurochemical Characteristics of Enteric Nervous System (ENS) Neurons in the Porcine Duodenum
Authors: Palus Katarzyna , Całka Jarosław
Published: December 2019

The digestive tract, especially the small intestine, is one of the main routes of acrylamide absorption and is therefore highly exposed to the toxic effect of acrylamide contained in food. The aim of this experiment was to elucidate the effect of low (tolerable daily intake—TDI) and high (ten times higher than TDI) doses of acrylamide on the neurochemical phenotype of duodenal enteric nervous system (ENS) neurons using the pig as an animal model. The experiment was performed on 15 immature gilts of the Danish Landrace assigned to three experimental groups: control (C) group—pigs administered empty gelatine capsules, low dose (LD) group—pigs administered capsules with acrylamide at the TDI dose (0.5 μg/kg body weight (b.w.)/day), and the high dose (HD) group—pigs administered capsules with acrylamide at a ten times higher dose than the TDI (5 μg/kg b.w./day) with a morning feeding for 4 weeks. Administration of acrylamide, even in a low (TDI) dose, led to an increase in the percentage of enteric neurons immunoreactive to substance P (SP), calcitonin gene-related peptide (CGRP), galanin (GAL), neuronal nitric oxide synthase (nNOS), and vesicular acetylcholine transporter (VACHT) in the porcine duodenum. The severity of the changes clearly depended on the dose of acrylamide and the examined plexus. The obtained results suggest the participation of these neuroactive substances in acrylamide-inducted plasticity and the protection of ENS neurons, which may be an important line of defence from the harmful action of acrylamide.
GTP Binding Is Necessary for the Activation of a Toxic Mutant Isoform of the Essential GTPase ObgE
Authors: Dewachter Liselot , Deckers Babette , Martin Ella , Herpels Pauline , Gkekas Sotirios , Versées Wim , Verstraeten Natalie , Fauvart Maarten , Michiels Jan
Published: December 2019

Even though the Obg protein is essential for bacterial viability, the cellular functions of this universally conserved GTPase remain enigmatic. Moreover, the influence of GTP and GDP binding on the activity of this protein is largely unknown. Previously, we identified a mutant isoform of ObgE (the Obg protein of Escherichia coli) that triggers cell death. In this research we explore the biochemical requirements for the toxic effect of this mutant ObgE* isoform, using cell death as a readily accessible read-out for protein activity. Both the absence of the N-terminal domain and a decreased GTP binding affinity neutralize ObgE*-mediated toxicity. Moreover, a deletion in the region that connects the N-terminal domain to the G domain likewise abolishes toxicity. Taken together, these data indicate that GTP binding by ObgE* triggers a conformational change that is transmitted to the N-terminal domain to confer toxicity. We therefore conclude that ObgE*–GTP, but not ObgE*–GDP, is the active form of ObgE* that is detrimental to cell viability. Based on these data, we speculate that also for wild-type ObgE, GTP binding triggers conformational changes that affect the N-terminal domain and thereby control ObgE function.
Use of QSAR Global Models and Molecular Docking for Developing New Inhibitors of c-src Tyrosine Kinase
Authors: Ancuceanu Robert , Tamba Bogdan , Stoicescu Cristina Silvia , Dinu Mihaela
Published: December 2019

A prototype of a family of at least nine members, cellular Src tyrosine kinase is a therapeutically interesting target because its inhibition might be of interest not only in a number of malignancies, but also in a diverse array of conditions, from neurodegenerative pathologies to certain viral infections. Computational methods in drug discovery are considerably cheaper than conventional methods and offer opportunities of screening very large numbers of compounds in conditions that would be simply impossible within the wet lab experimental settings. We explored the use of global quantitative structure-activity relationship (QSAR) models and molecular ligand docking in the discovery of new c-src tyrosine kinase inhibitors. Using a dataset of 1038 compounds from ChEMBL database, we developed over 350 QSAR classification models. A total of 49 models with reasonably good performance were selected and the models were assembled by stacking with a simple majority vote and used for the virtual screening of over 100,000 compounds. A total of 744 compounds were predicted by at least 50% of the QSAR models as active, 147 compounds were within the applicability domain and predicted by at least 75% of the models to be active. The latter 147 compounds were submitted to molecular ligand docking using AutoDock Vina and LeDock, and 89 were predicted to be active based on the energy of binding.
Molecular Signaling Regulating Endometrium–Blastocyst Crosstalk
Authors: Massimiani Micol , Lacconi Valentina , La Civita Fabio , Ticconi Carlo , Rago Rocco , Campagnolo Luisa
Published: December 2019

Implantation of the embryo into the uterine endometrium is one of the most finely-regulated processes that leads to the establishment of a successful pregnancy. A plethora of factors are released in a time-specific fashion to synchronize the differentiation program of both the embryo and the endometrium. Indeed, blastocyst implantation in the uterus occurs in a limited time frame called the “window of implantation” (WOI), during which the maternal endometrium undergoes dramatic changes, collectively called “decidualization”. Decidualization is guided not just by maternal factors (e.g., estrogen, progesterone, thyroid hormone), but also by molecules secreted by the embryo, such as chorionic gonadotropin (CG) and interleukin-1β (IL-1 β), just to cite few. Once reached the uterine cavity, the embryo orients correctly toward the uterine epithelium, interacts with specialized structures, called pinopodes, and begins the process of adhesion and invasion. All these events are guided by factors secreted by both the endometrium and the embryo, such as leukemia inhibitory factor (LIF), integrins and their ligands, adhesion molecules, Notch family members, and metalloproteinases and their inhibitors. The aim of this review is to give an overview of the factors and mechanisms regulating implantation, with a focus on those involved in the complex crosstalk between the blastocyst and the endometrium.
Cell-Based Mechanosensation, Epigenetics, and Non-Coding RNAs in Progression of Cardiac Fibrosis
Authors: Ferrari Silvia , Pesce Maurizio
Published: December 2019

The heart is par excellence the ‘in-motion’ organ in the human body. Compelling evidence shows that, besides generating forces to ensure continuous blood supply (e.g., myocardial contractility) or withstanding passive forces generated by flow (e.g., shear stress on endocardium, myocardial wall strain, and compression strain at the level of cardiac valves), cells resident in the heart respond to mechanical cues with the activation of mechanically dependent molecular pathways. Cardiac stromal cells, most commonly named cardiac fibroblasts, are central in the pathologic evolution of the cardiovascular system. In their normal function, these cells translate mechanical cues into signals that are necessary to renew the tissues, e.g., by continuously rebuilding the extracellular matrix being subjected to mechanical stress. In the presence of tissue insults (e.g., ischemia), inflammatory cues, or modifiable/unmodifiable risk conditions, these mechanical signals may be ‘misinterpreted’ by cardiac fibroblasts, giving rise to pathology programming. In fact, these cells are subject to changing their phenotype from that of matrix renewing to that of matrix scarring cells—the so-called myo-fibroblasts—involved in cardiac fibrosis. The links between alterations in the abilities of cardiac fibroblasts to ‘sense’ mechanical cues and molecular pathology programming are still under investigation. On the other hand, various evidence suggests that cell mechanics may control stromal cells phenotype by modifying the epigenetic landscape, and this involves specific non-coding RNAs. In the present contribution, we will provide examples in support of this more integrated vision of cardiac fibrotic progression based on the decryption of mechanical cues in the context of epigenetic and non-coding RNA biology.
Possible Contribution of Inflammation-Associated Hypoxia to Increased K2P5.1 K+ Channel Expression in CD4+ T Cells of the Mouse Model for Inflammatory Bowel Disease
Authors: Endo Kyoko , Kito Hiroaki , Tanaka Ryo , Kajikuri Junko , Tanaka Satoshi , Elboray Elghareeb E. , Suzuki Takayoshi , Ohya Susumu
Published: December 2019

Previous studies have reported the up-regulation of the two-pore domain K+ channel K2P5.1 in the CD4+ T cells of patients with multiple sclerosis (MS) and rheumatoid arthritis (RA), as well as in a mouse model of inflammatory bowel disease (IBD). However, the mechanisms underlying this up-regulation remain unclear. Inflammation-associated hypoxia is involved in the pathogenesis of autoimmune diseases, such as IBD, MS, and RA, and T cells are exposed to a hypoxic environment during their recruitment from inflamed tissues to secondary lymphoid tissues. We herein investigated whether inflammation-associated hypoxia is attributable to the increased expression and activity of K2P5.1 in the splenic CD4+ T cells of chemically-induced IBD model mice. Significant increases in hypoxia-inducible factor (HIF)-1α transcripts and proteins were found in the splenic CD4+ T cells of the IBD model. In the activated splenic CD4+ T cells, hypoxia (1.5% O2) increased K2P5.1 expression and activity, whereas a treatment with the HIF inhibitor FM19G11 but not the selective HIF-2 inhibitor exerted the opposite effect. Hypoxia-exposed K2P5.1 up-regulation was also detected in stimulated thymocytes and the mouse T-cell line. The class III histone deacetylase sirtuin-1 (SIRT1) is a downstream molecule of HIF-1α signaling. We examined the effects of the SIRT1 inhibitor NCO-01 on K2P5.1 transcription in activated CD4+ T cells, and we found no significant effects on the K2P5.1 transcription. No acute compensatory responses of K2P3.1–K2P5.1 up-regulation were found in the CD4+ T cells of the IBD model and the hypoxia-exposed T cells. Collectively, these results suggest a mechanism for K2P5.1 up-regulation via HIF-1 in the CD4+ T cells of the IBD model.
Combined Linkage Mapping and BSA to Identify QTL and Candidate Genes for Plant Height and the Number of Nodes on the Main Stem in Soybean
Authors: Li Ruichao , Jiang Hongwei , Zhang Zhanguo , Zhao Yuanyuan , Xie Jianguo , Wang Qiao , Zheng Haiyang , Hou Lilong , Xiong Xin , Xin Dawei , Hu Zhenbang , Liu Chunyan , Wu Xiaoxia , Chen Qingshan
Published: December 2019

Soybean is one of the most important food and oil crops in the world. Plant height (PH) and the number of nodes on the main stem (NNMS) are quantitative traits closely related to soybean yield. In this study, we used 208 chromosome segment substitution lines (CSSL) populations constructed using “SN14” and “ZYD00006” for quantitative trait locus (QTL) mapping of PH and NNMS. Combined with bulked segregant analysis (BSA) by extreme materials, 8 consistent QTLs were identified. According to the gene annotation of the QTL interval, a total of 335 genes were obtained. Five of which were associated with PH and NNMS, potentially representing candidate genes. RT-qPCR of these 5 candidate genes revealed two genes with differential relative expression levels in the stems of different materials. Haplotype analysis showed that different single nucleotide polymorphisms (SNPs) between the excellent haplotypes in Glyma.04G251900 and Glyma.16G156700 may be the cause of changes in these traits. These results provide the basis for research on candidate genes and marker-assisted selection (MAS) in soybean breeding.
Role of Claudin Proteins in Regulating Cancer Stem Cells and Chemoresistance-Potential Implication in Disease Prognosis and Therapy
Authors: Gowrikumar Saiprasad , Singh Amar B. , Dhawan Punita
Published: December 2019

Claudins are cell–cell adhesion proteins, which are expressed in tight junctions (TJs), the most common apical cell-cell adhesion. Claudin proteins help to regulate defense and barrier functions, as well as differentiation and polarity in epithelial and endothelial cells. A series of studies have now reported dysregulation of claudin proteins in cancers. However, the precise mechanisms are still not well understood. Nonetheless, studies have clearly demonstrated a causal role of multiple claudins in the regulation of epithelial to mesenchymal transition (EMT), a key feature in the acquisition of a cancer stem cell phenotype in cancer cells. In addition, claudin proteins are known to modulate therapy resistance in cancer cells, a feature associated with cancer stem cells. In this review, we have focused primarily on highlighting the causal link between claudins, cancer stem cells, and therapy resistance. We have also contemplated the significance of claudins as novel targets in improving the efficacy of cancer therapy. Overall, this review provides a much-needed understanding of the emerging role of claudin proteins in cancer malignancy and therapeutic management.
DRG2 Deficient Mice Exhibit Impaired Motor Behaviors with Reduced Striatal Dopamine Release
Authors: Lim Hye Ryeong , Vo Mai-Tram , Kim Dong Jun , Lee Unn Hwa , Yoon Jong Hyuk , Kim Hyung-Jun , Kim Jeongah , Kim Sang Ryong , Lee Jun Yeon , Yang Chae Ha , Kim Hee Young , Choi June-Seek , Kim Kijeong , Yang Esther , Kim Hyun , Lee Seongsoo , Lee Byung Ju , Kim Kyungjin , Park Jeong Woo , Ha Chang Man
Published: December 2019

Developmentally regulated GTP-binding protein 2 (DRG2) was first identified in the central nervous system of mice. However, the physiological function of DRG2 in the brain remains largely unknown. Here, we demonstrated that knocking out DRG2 impairs the function of dopamine neurons in mice. DRG2 was strongly expressed in the neurons of the dopaminergic system such as those in the striatum (Str), ventral tegmental area (VTA), and substantia nigra (SN), and on neuronal cell bodies in high-density regions such as the hippocampus (HIP), cerebellum, and cerebral cortex in the mouse brain. DRG2 knockout (KO) mice displayed defects in motor function in motor coordination and rotarod tests and increased anxiety. However, unexpectedly, DRG2 depletion did not affect the dopamine (DA) neuron population in the SN, Str, or VTA region or dopamine synthesis in the Str region. We further demonstrated that dopamine release was significantly diminished in the Str region of DRG2 KO mice and that treatment of DRG2 KO mice with l-3,4-dihydroxyphenylalanine (L-DOPA), a dopamine precursor, rescued the behavioral motor deficiency in DRG2 KO mice as observed with the rotarod test. This is the first report to identify DRG2 as a key regulator of dopamine release from dopamine neurons in the mouse brain.
Effects of Regorafenib, a Multi-Kinase Inhibitor, on Conjunctival Scarring in a Canine Filtration Surgery Model in Comparison with Mitomycin-C
Authors: Nemoto Emika , Kojima Shota , Sugiyama Tetsuya , Jin Denan , Takai Shinji , Maeda Michiko , Kohmoto Ryohsuke , Ueki Mari , Oku Hidehiro , Ikeda Tsunehiko
Published: December 2019

Regorafenib eye drops were developed for treating age-related macular degeneration. This study aimed to investigate the effects of this multi-kinase inhibitor on intraocular pressure (IOP), bleb formation, and conjunctival changes in a canine filtration surgery model. Glaucoma filtration surgery models were created in 24 eyes of 24 beagles. In experiment 1 (Ex 1), regorafenib eye drops (regorafenib group: n = 6) or a vehicle (control group, n = 6) were instilled twice daily for 4 weeks postoperatively. In experiment 2 (Ex 2), regorafenib eye drops were instilled as in Ex 1 (regorafenib group: n = 6) for 12 weeks while conventional intraoperative mitomycin-C (MMC) was utilized (MMC group: n = 6), In Ex 1, only the regorafenib group showed significant IOP reduction with a significantly higher bleb score. Subconjunctival area, collagen density, vessels, and cells showing proliferation and differentiation were lower in subconjunctival tissue in the regorafenib group. In Ex 2, no significant difference was found in IOP reduction and bleb formation between the regorafenib and MMC groups; bleb walls were significantly thicker and collagen density and vessels were higher in the regorafenib group; and no differences were observed in the above-mentioned cells. Thus, regorafenib might be a better alternative to MMC for creating thicker and less ischemic blebs in glaucoma filtration surgery.
Anti-Wrinkle Benefits of Peptides Complex Stimulating Skin Basement Membrane Proteins Expression
Authors: Jeong Sekyoo , Yoon Seokjeong , Kim Sungwoo , Jung Juyeon , Kor Myungho , Shin Kayoung , Lim Chaejin , Han Hyo Sun , Lee Haekwang , Park Kyeong-Yong , Kim Jinwan , Chung Hwa Jee , Kim Hyun Jung
Published: December 2019

The dermal-epidermal junction (DEJ) provides a physical and biological interface between the epidermis and the dermis. In addition to providing a structural integrity, the DEJ also acts as a passageway for molecular transport. Based on the recently reported importance of the DEJ in skin aging, novel peptide derivatives have been tested for their effects on basement membrane (BM) protein expressions in cultured human epidermal keratinocytes. As a result, protein expressions of collagen XVII, laminin and nidogen were stimulated by the test peptide and peptides complex. Further ex vivo evaluation using excised human skin, confirmed that the topical application of the peptides complex significantly increased dermal collagen expression, as well as expressions of collagen XVII and laminin. Interestingly, while the origin of the laminin protein is epidermal keratinocytes, the immunohistochemical staining of skin showed that laminin was only detected in the uppermost layer of the dermis, which suggests a tight assembly of laminin protein onto the dermal side of the DEJ. These results suggest that a peptide complex could improve the structural properties of the DEJ through its ability to stimulate BM proteins. In order to evaluate the anti-wrinkle benefits of the peptide complex in vivo, a clinical study was performed on 22 healthy Asian female volunteers older than 40 years. As a result, significant improvements in skin wrinkles for all of the five sites were observed after two weeks, as assessed by skin topographic measurements. Collectively, these results demonstrate the anti-aging efficacy of the peptides complex.
Grey Wolf Algorithm and Multi-Objective Model for the Manycast RSA Problem in EONs
Authors: Xuan Hejun , Lin Lidan , Qiao Lanlan , Zhou Yang
Published: December 2019

Manycast routing and spectrum assignment (RSA) in elastic optical networks (EONs) has become a hot research field. In this paper, the mathematical model and high efficient algorithm to solve this challenging problem in EONs is investigated. First, a multi-objective optimization model, which minimizes network power consumption, the total occupied spectrum, and the maximum index of used frequency spectrum, is established. To handle this multi-objective optimization model, we integrate these three objectives into one by using a weighted sum strategy. To make the population distributed on the search domain uniformly, a uniform design method was developed. Based on this, an improved grey wolf optimization method (IGWO), which was inspired by PSO (Particle Swarm Optimization, PSO) and DE (Differential Evolution, DE), is proposed to solve the maximum model efficiently. To demonstrate high performance of the designed algorithm, a series of experiments are conducted using several different experimental scenes. Experimental results indicate that the proposed algorithm can obtain better results than the compared algorithm.
Weakly Supervised Learning for Evaluating Road Surface Condition from Wheelchair Driving Data
Authors: Watanabe Takumi , Takahashi Hiroki , Iwasawa Yusuke , Matsuo Yutaka , Eguchi Yairi Ikuko
Published: December 2019

Providing accessibility information about sidewalks for people with difficulties with moving is an important social issue. We previously proposed a fully supervised machine learning approach for providing accessibility information by estimating road surface conditions using wheelchair accelerometer data with manually annotated road surface condition labels. However, manually annotating road surface condition labels is expensive and impractical for extensive data. This paper proposes and evaluates a novel method for estimating road surface conditions without human annotation by applying weakly supervised learning. The proposed method only relies on positional information while driving for weak supervision to learn road surface conditions. Our results demonstrate that the proposed method learns detailed and subtle features of road surface conditions, such as the difference in ascending and descending of a slope, the angle of slopes, the exact locations of curbs, and the slight differences of similar pavements. The results demonstrate that the proposed method learns feature representations that are discriminative for a road surface classification task. When the amount of labeled data is 10% or less in a semi-supervised setting, the proposed method outperforms a fully supervised method that uses manually annotated labels to learn feature representations of road surface conditions.
Rating Prediction Algorithm Based on User Time-Sensitivity
Authors: Cheng Shulin , Wang Wanyan
Published: December 2019

Rating prediction is an important technology in the personalized recommendation field. Prediction results are influenced by many factors, such as time, and their accuracy directly affects the quality of the recommendation. Current time-based collaborative filtering (CF) algorithms have improved the technology of prediction accuracy to a certain extent, but they fail to differentiate the time-sensitivity of different users, which further affects prediction accuracy. To address this issue, we have proposed a rating prediction algorithm based on user time-sensitivity differences. First, we analyzed and modeled the time sensitivities of users, utilized cosine distance and relative entropy to build a judgment function, and then judged the time sensitivities of users based on a voting strategy. Next, we applied the time-sensitivity difference to improve the traditional CF algorithm and optimized the combination of parameters. Finally, we tested our algorithm on standard datasets. The experimental results showed that there are many users who have different sensitivities to time. According to these experimental results, our proposed algorithm has achieved a higher prediction accuracy than other state-of-the-art algorithms.
Complex q-Rung Orthopair Fuzzy Aggregation Operators and Their Applications in Multi-Attribute Group Decision Making
Authors: Liu Peide , Mahmood Tahir , Ali Zeeshan
Published: December 2019

In this manuscript, the notions of q-rung orthopair fuzzy sets (q-ROFSs) and complex fuzzy sets (CFSs) are combined is to propose the complex q-rung orthopair fuzzy sets (Cq-ROFSs) and their fundamental laws. The Cq-ROFSs are an important way to express uncertain information, and they are superior to the complex intuitionistic fuzzy sets and the complex Pythagorean fuzzy sets. Their eminent characteristic is that the sum of the qth power of the real part (similarly for imaginary part) of complex-valued membership degree and the qth power of the real part (similarly for imaginary part) of complex-valued non‐membership degree is equal to or less than 1, so the space of uncertain information they can describe is broader. Under these environments, we develop the score function, accuracy function and comparison method for two Cq-ROFNs. Based on Cq-ROFSs, some new aggregation operators are called complex q-rung orthopair fuzzy weighted averaging (Cq-ROFWA) and complex q-rung orthopair fuzzy weighted geometric (Cq-ROFWG) operators are investigated, and their properties are described. Further, based on proposed operators, we present a new method to deal with the multi‐attribute group decision making (MAGDM) problems under the environment of fuzzy set theory. Finally, we use some practical examples to illustrate the validity and superiority of the proposed method by comparing with other existing methods.
M[B2(SO4)4] (M = Mn, Zn)—Syntheses and Crystal Structures of Two New Phyllosilicate Analogue Borosulfates
Authors: Pasqualini Leonard C. , Huppertz Hubert , Bruns Jörn
Published: December 2019

Borosulfates are a rapidly expanding class of silicate analogue materials, where the structural diversity is expected to be at least as large as known for silicates. However, borosulfates with cross-linking of the anionic network into two or even three dimensions are still very rare. Herein, we present two new representatives with phyllosilicate analogue topology. Through solvothermal reactions of ZnO and MnCl2∙4H2O with boric acid in oleum (65% SO3), we obtained single-crystals of Mn[B2(SO4)4] (monoclinic, P21/n, Z = 2, a = 8.0435(4), b = 7.9174(4), c = 9.3082(4) Å, β = 110.94(1)°, V = 553.63(5) Å3) and Zn[B2(SO4)4] (monoclinic, P21/n, Z = 2, a = 7.8338(4), b = 8.0967(4), c = 9.0399(4) Å, β = 111.26(1)°, V = 534.36(5) Å3). The crystal structures reveal layer-like anionic networks with alternating vierer- and zwölfer-rings formed exclusively by corner-linked (SO4)- and (BO4)-tetrahedra.
The Effect of Longwave Ultraviolet Light Radiation on Dendrolimus tabulaeformis Antioxidant and Detoxifying Enzymes
Authors: Wang Wenlong , Gao Chenglong , Ren Lili , Luo Youqing
Published: December 2019

Longwave ultraviolet (UVA) light, in the range of 315–400 nm, has been widely used as a light source in the light trapping of insect pests. Previous studies have demonstrated the oxidative stress and lethal effect of UV radiation on insects. In this study, we evaluated the influence of UVA radiation on the antioxidant and detoxifying enzymes of Dendrolimus tabulaeformis. We tested the contents of malondialdehyde (MDA), hydroxyl radical (·OH), hydrogen peroxide (H2O2), reduced glutathione (GSH), and oxidized glutathione (GSSH) following different exposure time periods of UVA light irradiation on D. tabulaeformis adults. In addition, we investigated how the activities of antioxidant and detoxifying enzymes responded to UVA radiation by determining the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), polyphenol oxidase (PPO), glutathione S-transferase (GST), glutathione reductase (GR), acetylcholinesterase (AChE), carboxylesterase (CarE), alkaline phosphatase (ALP), and acid phosphatase (ACP). Adults were exposed to UVA light for different time periods (0, 5, 15, 30, 60, and 120 min). We found that exposure to UVA light for 5 min resulted in rapid variation in the activities of the antioxidant and detoxification enzyme systems. However, the antioxidant capacity of females was incongruous with that of males following UVA irradiation. Our results confirmed that UVA light irradiation increased the level of oxidative stress and disturbed physiological detoxification in D. tabulaeformis adults. Based on the above results, we anticipated that further research of the mechanism of UVA irradiation on the antioxidant and detoxifying enzymes of D. tabulaeformis would gain more importance, allowing to develop and use new, less toxic and environmentally friendly pesticides.
Seasonal Occurrence of Potato Psyllid (Bactericera Cockerelli) and Risk of Zebra Chip Pathogen (Candidatus Liberibacter Solanacearum) in Northwestern New Mexico
Authors: Djaman Koffi , Higgins Charles , Begay Shantel , Koudahe Komlan , Allen Samuel , Lombard Kevin , O’Neill Michael
Published: December 2019

Potato psyllid (Bactericera cockerelli) is one of the most important pests in potatoes (Solanum tuberosum L.) due to its feeding behavior and the transmission of a bacterium (Candidatus Liberibacter solanacearum) that causes zebra chip disease, altering the quality of the potato tuber and the fried potato chip or french fry. This pest is thus a threat to the chip potato industry and often requires preventive measures including the use of costly insecticides. The objectives of this research were to monitor the variation in B. cockerelli adult abundance and to evaluate the risk of zebra chip disease in northwestern New Mexico, USA. Yellow sticky traps were used to collect the pest at the Agricultural Experiment Station at Farmington, NM and in nearby commercial fields at the Navajo Agricultural Products Industry (NAPI) and Navajo Mesa Farms during the 2017–2019 period. The collected adult pests were analyzed at Texas A & M University for the presence of Candidatus L. solanacearum (Lso). The results showed field infestation by B. cockerelli in early June and that the population peaked during the second half of July and decreased as the potato growing season progressed. However, a second less important peak of the pest was revealed around mid- to late-August, depending on the growing season and field. While the B. cockerelli population increased linearly with average air temperature, it showed strong third order polynomial relationships with the accumulated thermal units and the Julian days. The test of B. cockerelli for the Lso infection revealed a low incidence of the pathogen varying from 0.22% to 6.25% and the infected adult B. cockerelli were collected during the population peak period. The results of this study may be helpful to potato growers in pest management decision-making and control. However, more study is needed to evaluate zebra chip disease in terms of its prevention and economic impact, and to develop economic thresholds and pest management programs for northwestern New Mexico and neighboring regions.
Comparative Quantification of Trail-Following Behavior in Pest Ants
Authors: Vázquez Ricardo J. , Koehler Philip G. , Pereira Roberto M.
Published: December 2019

A comparison of trail-following movement parameters of six major urban pest ants, Nylanderia fulva (Forel) (Hymenoptera: Formicidae), Pheidole megacephala, Linepithema humile (Mayr), Solenopsis invicta Buren, Paratrechina longicornis (Forel), and Technomyrmex albipes (Smith) demonstrated several differences in velocity of movement, amplitude of the deviations from a marked trail, percent fidelity to the trail, number of curves per unit of trail, and trail-following accuracy. Paratrechina longicornis and N. fulva had the largest deviations from the marked trails and moved three times faster (25–30 mm/s) along the trail than the slowest ant, S. invicta (< 10 mm/s), with other ants following between these extremes. Species differences in relation to going toward or returning from food were observed in a few cases, especially with Pa. longicornis for which velocity, amplitude, and trail fidelity differed between the foraging and return trails. Quantification of ant trail-following movement parameters can be useful in understanding the mechanics of ant movement and may be important in testing specific strategies and products that disrupt trail-following behavior.
“Generalist” Aphid Parasitoids Behave as Specialists at the Agroecosystem Scale
Authors: Derocles Stéphane A.P. , Navasse Yoann , Buchard Christelle , Plantegenest Manuel , Le Ralec Anne
Published: December 2019

The degree of trophic specialization of interacting organisms impacts on the structure of ecological networks and has consequences for the regulation of crop pests. However, it remains difficult to assess in the case of parasitoids. Host ranges are often established by listing host records from various years and geographic areas in the literature. Here, we compared the actual hosts exploited at a local farm-scale by aphid parasitoids (Hymenoptera: Aphidiinae), to the available species listed as hosts for each parasitoid species. We sampled aphids and their parasitoids in cultivated and uncultivated areas in an experimental farm from April to November 2014 and thereafter used DNA-based data to determine whether a differentiation in sequences existed. Twenty-nine parasitoid species were found on 47 potential aphid hosts. Our results showed that the great majority of the parasitoid tested used fewer host species than expected according to data published in the literature and parasitized a limited number of hosts even when other potential hosts were available in the environment. Moreover, individuals of the most generalist species differed in their DNA sequences, according to the aphid species and/or the host plant species. At a local scale, only obligate or facultative specialist aphid parasitoids were detected. Local specialization has to be considered when implementing the use of such parasitoids in pest regulation within agroecosystems.
Bioaccumulation of Cadmium Affects Development, Mating Behavior, and Fecundity in the Asian Corn Borer, Ostrinia furnacalis
Authors: Luo Mei , Cao Hong-Mei , Fan Ying-Ying , Zhou Xiao-Cao , Chen Jun-Xian , Chung Henry , Wei Hong-Yi
Published: December 2019

Heavy metal pollution is becoming an increasingly serious problem in agricultural ecosystems. Heavy metals such as cadmium (Cd) accumulate in the food chain and may lead to detrimental effects on the physiological functions of living organisms, including herbivorous insects. One such example is the Asian Corn Borer, Ostrinia furnacalis (Lepidoptera: Pyralidae). However, how Cd can affect the development and reproduction of O. furnacalis is largely unknown. In this study, we exposed larvae of O. furnacalis to a diet containing Cd and investigated the effects of Cd on the development, mating behavior, and fecundity of the insect. We showed that Cd accumulates in the larvae and inhibits development by extending larval and pupal duration and decreasing the survival rate. The excretion of Cd through multiple routes during the larval and pupal stages resulted in low levels of residual Cd in the adult insects, which were not fed with Cd. However, the mating behavior and fecundity of these insects were significantly affected, compared to control insects. This suggests that the bioaccumulation of heavy metals such as Cd has long lasting and detrimental effects on O. furnacalis over the entire life cycle, affecting fecundity, even when specimens are only exposed at an early life stage.
Balance Performance across the Lifespan Assessed by the Leonardo Mechanograph®: A Cross-Sectional Study
Authors: Wiegmann Sabine , Felsenberg Dieter , Gast Ulf , Börst Hendrikje , Armbrecht Gabriele , Dietzel Roswitha
Published: December 2019

Reference values of sway parameters have not been published for the Leonardo mechanograph® so far. The aim of this cross-sectional study was to determine normative values on postural control measured by the force plate Leonardo Mechanograph® and to analyze the influence of age and sex on balance performance. A set of standardized standing positions with eyes opened (Romberg, semi-tandem, tandem, unipedal standing) was carried out. Analysis of covariance (ANCOVA) was used to detect age-and sex-related differences in center of pressure (COP) parameters (path length, velocity, elliptical area, anterior-posterior, and medio-lateral directions). Measurements were available for 570 subjects aged 20–86 years. Statistical analysis showed a high effect of age group on postural control (partial n² between 0.1 and 0.4) with a U-shaped dependency between postural control and age for all area- and path-related COP parameters, with the largest sway in the youngest (aged 20–40) and the oldest age group (aged 60–86). For velocity of COP, a linear deterioration with increasing age was found. Medio-lateral components of COP are likely to indicate the extent of postural control. Significant sex differences were not clearly supported by current findings. Age- and sex-related normative values are a useful resource for diagnostic, research, and training.
Bioconcentration of Essential and Nonessential Elements in Black Sea Turbot (Psetta Maxima Maeotica Linnaeus, 1758) in Relation to Fish Gender
Authors: Simionov Ira-Adeline , Cristea Victor , Petrea Stefan-Mihai , Mogodan Alina , Nicoara Mircea , Baltag Emanuel Stefan , Strungaru Stefan-Adrian , Faggio Caterina
Published: December 2019

This study investigates the influence of gender in the bioconcentration of essential and nonessential elements in different parts of Black Sea turbot (Psetta maxima maeotica) body, from an area considered under high anthropogenic pressure (the Constanta City Black Sea Coastal Area in Romania). A number of 13 elements (Ca, Mg, Na, K, Fe, Zn, Mn, Cu, Ni, Cr, As, Pb and Cd) were measured in various sample types: muscle, stomach, stomach content, intestine, intestine content, gonads, liver, spleen, gills and caudal fin. Turbot adults (4–5 years old) were separated, according to their gender, into two groups (20 males, 20 females, respectively), and a high total number of samples (1200 from both groups) were prepared and analyzed, in triplicate, with Flame Atomic Absorption Spectrometry and High-Resolution Continuum Source Atomic Absorption Spectrometry with Graphite Furnace techniques. The results were statistically analyzed in order to emphasize the bioconcentration of the determined elements in different tissues of wild turbot males vs. females, and also to contribute to an upgraded characterization of the Romanian Black Sea Coast, around Constanta City, in terms of heavy metals pollution. The essential elements Mg and Zn have different roles in the gonads of males and females, as they were the only elements with completely different patterns between the analyzed groups of specimens. The concentrations of studied elements in muscle were not similar with the data provided by literature, suggesting that chemistry of the habitat and food plays a major role in the availability of the metals in the body of analyzed fish species. The gender influenced the bioaccumulation process of all analyzed elements in most tissues since turbot male specimens accumulated higher concentration of metals compared to females. The highest bioaccumulation capacity in terms of Ca, Mg, Na, Ni, As, Zn and Cd was registered in caudal fin, liver and intestine tissues. Also, other elements such as K, Fe, Cu and Mn had the highest bioaccumulation in their muscle, spleen, liver and gills tissues. The concentrations of toxic metals in Black Sea turbot from this study were lower in the muscle samples compared with the studies conducted in Turkey, suggesting that the anthropogenic activity in the studied area did not pose a major impact upon the habitat contamination.
Sea Waves Transport of Inertial Micro-Plastics: Mathematical Model and Applications
Authors: Stocchino Alessandro , De Leo Francesco , Besio Giovanni
Published: December 2019

Plastic pollution in seas and oceans has recently been recognized as one of the most impacting threats for the environment, and the increasing number of scientific studies proves that this is an issue of primary concern. Being able to predict plastic paths and concentrations within the sea is therefore fundamental to properly face this challenge. In the present work, we evaluated the effects of sea waves on inertial micro-plastics dynamics. We hypothesized a stationary input number of particles in a given control volume below the sea surface, solving their trajectories and distributions under a second-order regular wave. We developed an exhaustive group of datasets, spanning the most plausible values for particles densities and diameters and wave characteristics, with a specific focus on the Mediterranean Sea. Results show how the particles inertia significantly affects the total transport of such debris by waves.
Local Scour for Vertical Piles in Steady Currents: Review of Mechanisms, Influencing Factors and Empirical Equations
Authors: Liang Bingchen , Du Shengtao , Pan Xinying , Zhang Libang
Published: December 2019

Scour induced by currents is one of the main causes of the bridge failure in rivers. Fundamental knowledge and mechanisms on scour processes due to currents are often taken as a basis for scour studies, which are the focus of this review. Scour development induced by waves and in combined wave–current conditions are also briefly discussed. For the design of structure foundations, the maximum scour depths need to be estimated. The mechanisms of local scour and predictions of maximum local scour depths have been studied extensively for many years. Despite the complexity of the scour process, a lot of satisfying results and progresses have been achieved by many investigators. In order to get a comprehensive review of local scour for vertical piles, major progresses made by researchers are summarized in this review. In particular, maximum scour depth influencing factors including flow intensity, sediments, pile parameters and time are analyzed with experimental data. A few empirical equations referring to temporary scour depth and maximum scour depth were classified with their expressing forms. Finally, conclusions and future research directions are addressed.
AIS-Based Multiple Vessel Collision and Grounding Risk Identification based on Adaptive Safety Domain
Authors: Bakdi Azzeddine , Glad Ingrid Kristine , Vanem Erik , Engelhardtsen Øystein
Published: December 2019

The continuous growth in maritime traffic and recent developments towards autonomous navigation have directed increasing attention to navigational safety in which new tools are required to identify real-time risk and complex navigation situations. These tools are of paramount importance to avoid potentially disastrous consequences of accidents and promote safe navigation at sea. In this study, an adaptive ship-safety-domain is proposed with spatial risk functions to identify both collision and grounding risk based on motion and maneuverability conditions for all vessels. The algorithm is designed and validated through extensive amounts of Automatic Identification System (AIS) data for decision support over a large area, while the integration of the algorithm with other navigational systems will increase effectiveness and ensure reliability. Since a successful evacuation of a potential vessel-to-vessel collision, or a vessel grounding situation, is highly dependent on the nearby maneuvering limitations and other possible accident situations, multi-vessel collision and grounding risk is considered in this work to identify real-time risk. The presented algorithm utilizes and exploits dynamic AIS information, vessel registry and high-resolution maps and it is robust to inaccuracies of position, course and speed over ground records. The computation-efficient algorithm allows for real-time situation risk identification at a large-scale monitored map up to country level and up to several years of operation with a very high accuracy.
The Role of Molecular Tests in the Diagnosis of Disseminated Histoplasmosis
Authors: Vasconcellos Izadora Clezar da Silva , Dalla Lana Daiane Flores , Pasqualotto Alessandro C.
Published: December 2019

Histoplasmosis is an emerging fungal disease, with global distribution. The disseminated form of the disease is a more severe infection, generally associated with AIDS. Classic diagnostic methods for histoplasmosis consist of microscopy, culture, and histopathology. More recently, the importance of Histoplasma antigen detection has dominated the literature on histoplasmosis diagnosis, but the relevance of molecular assays has not been as much studied. Here we describe the results of a systematic literature review focusing on studies that mainly compared immunological techniques (Histoplasma urine antigen detection) with molecular tests for the diagnosis of histoplasmosis. In addition to the review of comparative studies using such diagnostic techniques, the literature on polymerase chain reaction (PCR) tests in patients with disseminated histoplasmosis is also summarized. Two studies reported the comparison between immunological and molecular methods applied simultaneously for the diagnosis of disseminated histoplasmosis. PCR demonstrates a satisfactory performance assisting in the detection of Histoplasma spp. DNA in clinical samples.
Histoplasmosis in Persons Living with HIV
Authors: Nacher Mathieu
Published: December 2019

The increase in the number of immunocompromised persons, following the HIV pandemic, has led to a dramatic amplification of the number of patients with progressive disseminated histoplasmosis [...]
Biotic Environments Supporting the Persistence of Clinically Relevant Mucormycetes
Authors: Richardson Malcolm D. , Rautemaa-Richardson Riina
Published: December 2019

Clinically relevant members of the Mucorales group can grow and are found in diverse ecological spaces such as soil, dust, water, decomposing vegetation, on and in food, and in hospital environments but are poorly represented in mycobiome studies of outdoor and indoor air. Occasionally, Mucorales are found in water-damaged buildings. This mini review examines a number of specialised biotic environments, including those revealed by natural disasters and theatres of war, that support the growth and persistence of these fungi. However, we are no further forward in understanding exposure pathways or the chronicity of exposure that results in the spectrum of clinical presentations of mucormycosis.