bluuebunny/crossref_metadata_2025 huggingface dataset:
Rapid diagnosis of fatal Nocardia kroppenstedtii bacteremic pneumonia and empyema thoracis by next-generation sequencing: a case report
Authors: Xing Fanfan , Xia Yao , Lu Qianyun , Lo Simon K. F. , Lau Susanna K. P. , Woo Patrick C. Y.
Published: July 2023

Nocardia species do not replicate as rapidly as other pyogenic bacteria and nocardial infections can be highly fatal, particularly in immunocompromised patients. Here, we present the first report of fatal Nocardia kroppenstedtii bacteremic pneumonia and empyema thoracis diagnosed by next-generation sequencing (NGS) using the Oxford Nanopore Technologies' MinION device. The bacterium was not identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Due to its low equipment cost, short turn-around-time, and portable size, the Oxford Nanopore Technologies' MinION device is a useful platform for NGS in routine clinical microbiology laboratories.
Salt stress perception and metabolic regulation network analysis of a marine probiotic Meyerozyma guilliermondii GXDK6
Authors: Cai Xinghua , Sun Huijie , Yan Bing , Bai Huashan , Zhou Xing , Shen Peihong , Jiang Chengjian
Published: July 2023

Introduction

Extremely salt-tolerant microorganisms play an important role in the development of functional metabolites or drug molecules.

Methods

In this work, the salt stress perception and metabolic regulation network of a marine probiotic Meyerozyma guilliermondii GXDK6 were investigated using integrative omics technology.

Results

Results indicated that GXDK6 could accept the salt stress signals from signal transduction proteins (e.g., phosphorelay intermediate protein YPD1), thereby contributing to regulating the differential expression of its relevant genes (e.g., CTT1, SOD) and proteins (e.g., catalase, superoxide dismutase) in response to salt stress, and increasing the salt-tolerant viability of GXDK6. Omics data also suggested that the transcription (e.g., SMD2), translation (e.g., MRPL1), and protein synthesis and processing (e.g., inner membrane protein OXA1) of upregulated RNAs may contribute to increasing the salt-tolerant survivability of GXDK6 by improving protein transport activity (e.g., Small nuclear ribonucleoprotein Sm D2), anti-apoptotic ability (e.g., 54S ribosomal protein L1), and antioxidant activity (e.g., superoxide dismutase). Moreover, up to 65.9% of the differentially expressed genes/proteins could stimulate GXDK6 to biosynthesize many salt tolerant-related metabolites (e.g., β-alanine, D-mannose) and drug molecules (e.g., deoxyspergualin, calcitriol), and were involved in the metabolic regulation of GXDK6 under high NaCl stress.

Discussion

This study provided new insights into the exploration of novel functional products and/or drugs from extremely salt-tolerant microorganisms.
A novel organotypic cortical slice culture model for traumatic brain injury: molecular changes induced by injury and mesenchymal stromal cell secretome treatment
Authors: Pischiutta Francesca , Cavaleiro Helena , Caruso Enrico , Tribuzio Francesca , Di Marzo Noemi , Moro Federico , Kobeissy Firas , Wang Kevin K. , Salgado António J. , Zanier Elisa R.
Published: July 2023

Traumatic brain injury (TBI) is a major worldwide neurological disorder with no neuroprotective treatment available. Three-dimensional (3D) in vitro models of brain contusion serving as a screening platform for drug testing are lacking. Here we developed a new in vitro model of brain contusion on organotypic cortical brain slices and tested its responsiveness to mesenchymal stromal cell (MSC) derived secretome. A focal TBI was induced on organotypic slices by an electromagnetic impactor. Compared to control condition, a temporal increase in cell death was observed after TBI by propidium iodide incorporation and lactate dehydrogenase release assays up to 48 h post-injury. TBI induced gross neuronal loss in the lesion core, with disruption of neuronal arborizations measured by microtubule-associated protein-2 (MAP-2) immunostaining and associated with MAP-2 gene down-regulation. Neuronal damage was confirmed by increased levels of neurofilament light chain (NfL), microtubule associated protein (Tau) and ubiquitin C-terminal hydrolase L1 (UCH-L1) released into the culture medium 48 h after TBI. We detected glial activation with microglia cells acquiring an amoeboid shape with less ramified morphology in the contusion core. MSC-secretome treatment, delivered 1 h post-injury, reduced cell death in the contusion core, decreased NfL release in the culture media, promoted neuronal reorganization and improved microglia survival/activation. Our 3D in vitro model of brain contusion recapitulates key features of TBI pathology. We showed protective effects of MSC-secretome, suggesting the model stands as a tractable medium/high throughput, ethically viable, and pathomimetic biological asset for testing new cell-based therapies.
Diversity of aphantasia revealed by multiple assessments of visual imagery, multisensory imagery, and cognitive style
Authors: Takahashi Junichi , Saito Godai , Omura Kazufumi , Yasunaga Daichi , Sugimura Shinichiro , Sakamoto Shuichi , Horikawa Tomoyasu , Gyoba Jiro
Published: July 2023

Aphantasia—a condition wherein individuals have a reduced or absent construction of voluntary visual imagery—is diagnosed using either the Vividness of Visual Imagery Questionnaire (VVIQ) or self-identification. However, a significant discrepancy exists between the proportions of aphantasia in the populations assessed using these two criteria. It is unclear why the reported proportions differ excessively and what percentage of people cannot form visual imagery. We investigated the replicability of the proportion of people with aphantasia using both criteria in the same population of participants. Therefore, we explored the potential causes of the discrepancy and characteristics of putative aphantasia in terms of multisensory imagery, cognitive style, and face recognition ability. First, we conducted an online sampling study (Study 1: N = 2,871) using the VVIQ, self-identification of a reduction in visual imagery, Questionnaire upon Mental Imagery (QMI), and Verbalizer-Visualizer Questionnaire (VVQ). We found that 3.7 and 12.1% fulfilled the VVIQ and self-identification criteria, respectively, roughly replicating the proportions reported in previous studies. The self-identification criterion—but not the VVIQ criterion—contains items related to face recognition; hence, we suspected that face recognition ability was factor contributing to this discrepancy and conducted another online sampling study (Study 2: N = 774). We found a significant correlation between VVIQ and face recognition ability in the control group with self-identification, but not in the group defined by low VVIQ (VVIQ ≤32). As the participants in the control group with self-identification tended to exhibit moderately high VVIQ scores but low face recognition ability, we reason that the discrepancy can be partially explained by the contamination of individual differences in face recognition ability. Additional analyses of Study 1 revealed that the aphantasia group included participants who lacked all types of sensory imagery or only visual imagery in multisensory imagery and exhibited a non-specific cognitive style. This study indicates that the VVIQ alone may be insufficient to diagnose individuals who report an inability to form visual imagery. Furthermore, we highlight the importance of multiple assessments—along with the VVIQ—to better understand the diversity of imagery in aphantasia.
Mapping gender stereotypes: a network analysis approach
Authors: Sánchez-Rodríguez Ángel , Moreno-Bella Eva , García-Sánchez Efraín
Published: July 2023

Introduction

Stereotypes have traditionally been considered as “mental pictures” of a particular social group. The current research aims to draw the structure of gender stereotypes and metastereotype schemes as complex systems of stereotypical features. Therefore, we analyze gender stereotypes as networks of interconnected characteristics.

Method

Through an online survey (N = 750), participants listed the common female and male features to build the structure of the gender stereotypes. Participants also listed the common features of how members of one gender think they are viewed by people of the other gender to build the structure of gender metastereotypes.

Results

Our results suggest that female stereotypes are characterized by a single community of features consistently associated such as intelligent, strong, and hardworkers. Female metastereotype, however, combines the previous community with another characterized by weak and sensitive. On the contrary, the male stereotype projected by women is characterized by a community of features associated such as intelligent, strong, and hardworker, but male in-group stereotypes and metastereotypes projected by men are a combination of this community with another one characterized by features associated such as strong, chauvinist, and aggressive.

Discussion

A network approach to studying stereotypes provided insights into the meaning of certain traits when considered in combination with different traits. (e.g., strong-intelligent vs. strong-aggressive). Thus, focusing on central nodes can be critical to understanding and changing the structure of gender stereotypes.
Droplet microfluidic technologies for next-generation high-throughput screening
Authors: Ali Mushtaq , Kim Woohyuk , Park Jinsoo
Published: July 2023

Droplet microfluidics has evolved into a promising platform for high-throughput screening (HTS), allowing for rapid and precise analysis of thousands of samples encapsulated within droplets. Droplet microfluidic platform offers versatility, high-throughput, and the ability to compartmentalize reactions for a wide range of applications including pharmaceutics, cell analysis, and combinatorial chemical analysis. While droplet-based microfluidics has made considerable advances in automating basic laboratory tasks, for instance, manipulation, storage, and analysis, there has been comparatively little advancement toward HTS applications. The complexity of the technology, the lack of standardization, and the challenges associated with screening large numbers of samples are all factors that have contributed to the limited adoption of droplet-based microfluidics in HTS. In this perspective, we provide a comprehensive overview of the progress of droplet microfluidics as a potential platform for next-generation HTS, specifically in the domain of droplet separation and library generation. We hope that this perspective will inspire further research in relevant academic fields and contribute to the development of innovative HTS strategies based on droplet microfluidic technologies.
The complexity of the day-side X-line during southward interplanetary magnetic field
Authors: Burkholder Brandon L. , Chen Li-Jen , Sorathia Kareem , Sciola Anthony , Merkin Slava , Trattner Karlheinz J. , Gershman Daniel , Ma Xuanye , Connor Hyunju
Published: July 2023

High-resolution global magnetohydrodynamics (MHD) simulations include both meso- and global-scale processes occurring at the magnetopause, which interact to determine the time-dependent orientation of the day-side x-line (DXL). This study demonstrates that the global orientation of the DXL in GAMERA global MHD simulations varies on a time scale of minutes during steady southward interplanetary magnetic field conditions. This behavior manifests in observational data when reconnection outflows indicate that the direction to the x-line is opposite to the prediction from a steady-state model of the reconnection location. Because steady-state models of the DXL do not capture dynamics that are independent of solar wind variations, particularly surface waves and flux transfer events, they represent a time-averaged state of the system.
The role of the ankle plantar flexor muscles in trip recovery during walking: a computational modeling study
Authors: Namayeshi Tayebeh , Haddara Raneem , Ackland David , Lee Peter Vee Sin
Published: July 2023

Background

Reactive lower limb muscle function during walking plays a role in balance, stability, and ultimately fall prevention. The objective of this study was to evaluate muscle and joint function used to regain balance after trip-based perturbations during walking.

Research question

How are lower limb muscles used to recover from external tripping during walking?

Method

The dominant legs of 20 healthy adult participants with similar athletic backgrounds were tripped using a split-belt instrumented treadmill. High- and medium-intensity trips were simulated by deceleration of the dominant leg at initial contact from the speed of 1.1 m/s to 0 m/s and back to 1.1 m/s in 0.4 s and 0.8 s, respectively. Lower limb kinematics, kinetics, and muscle forces following perturbations were computed to pre-perturbation values using statistical parametric mapping (SPM) paired t-test.

Results

A greater ankle dorsiflexion angle (mean difference: 5.3°), ankle plantar flexion moment (mean difference: 0.6Nm/kg), and gastrocnemius and soleus muscle forces (mean difference: 4.27N/kg and 13.56N/kg for GAS and SOL, respectively) were observed post-perturbation step despite the magnitude of the perturbation.

Significance

This study concludes that adequate timely response of ankle function during a compensatory step is required for a successful recovery after tripping during walking in young healthy adults. Weakness in plantar flexors suggests insufficient ankle moments, which ultimately can result in falls. The findings of this paper can be used as a reference for the joint moments and range of motion needed to recover trips in the design of assistive devices. In addition to that, clinicians can use the estimated values of muscle forces and the pattern of muscle activities to design targeted training in fall prevention among the elderly.
Salbutamol transport and deposition in healthy cat airways under different breathing conditions and particle sizes
Authors: Fernández-Parra Rocio , Pey Pascaline , Reinero Carol , Malvè Mauro
Published: July 2023

Salbutamol is a bronchodilatator commonly used for the treatment of feline inflammatory lower airway disease, including asthma or acute bronchospasm. As in humans, a pressurized metered dose inhaler (pMDI) is used in conjunction with a spacer and a spherical mask to facilitate salbutamol administration. However, efficacy of inhalation therapy is influenced by different factors including the non-cooperative character of cats. In this study, the goal was to use computational fluid dynamics (CFD) to analyze the impact of breathing patterns and salbutamol particle size on overall drug transport and deposition using a specific spherical mask and spacer designed for cats. A model incorporating three-dimensional cat airway geometry, a commercially available spherical mask, and a 10 cm spacer, was used for CFD analysis. Two peak inspiratory flows were tested: 30 mL/s and 126 mL/s. Simulations were performed with 30s breathing different inspiratory and expiratory times, respiratory frequencies and peaks. Droplet spray transport and deposition were simulated with different particle sizes typical of the drug delivery therapies (1, 5, 10, and 15 μm). The percentage of particle deposition into the device and upper airways decreased with increasing particle diameter during both flows imposed in this cat model. During increased mean ventilatory rate (MVR) conditions, most of the salbutamol was lost in the upper airways. And during decreased MVR conditions, most of the particles remained in suspension (still in hold-up) between the mask and the carina, indicating the need for more than 30 s to be transported. In both flows the percentage of particles traveling to the lung was low at 1.5%–2.3%. In conclusion, in contrast to what has been described in the human literature, the results from this feline model suggest that the percentage of particles deposited on the upper airway decreases with increasing particle diameter.
Swine gut microbiome associated with non-digestible carbohydrate utilization
Authors: Pandey Sriniwas , Kim Eun Sol , Cho Jin Ho , Song Minho , Doo Hyunok , Kim Sheena , Keum Gi Beom , Kwak Jinok , Ryu Sumin , Choi Yejin , Kang Juyoun , Lee Jeong Jae , Kim Hyeun Bum
Published: July 2023

Non-digestible carbohydrates are an unavoidable component in a pig’s diet, as all plant-based feeds contain different kinds of non-digestible carbohydrates. The major types of non-digestible carbohydrates include non-starch polysaccharides (such as cellulose, pectin, and hemicellulose), resistant starch, and non-digestible oligosaccharides (such as fructo-oligosaccharide and xylo-oligosaccharide). Non-digestible carbohydrates play a significant role in balancing the gut microbial ecology and overall health of the swine by promoting the production of short chain fatty acids. Although non-digestible carbohydrates are rich in energy, swine cannot extract this energy on their own due to the absence of enzymes required for their degradation. Instead, they rely on gut microbes to utilize these carbohydrates for energy production. Despite the importance of non-digestible carbohydrate degradation, limited studies have been conducted on the swine gut microbes involved in this process. While next-generation high-throughput sequencing has aided in understanding the microbial compositions of the swine gut, specific information regarding the bacteria involved in non-digestible carbohydrate degradation remains limited. Therefore, it is crucial to investigate and comprehend the bacteria responsible for the breakdown of non-digestible carbohydrates in the gut. In this mini review, we have discussed the major bacteria involved in the fermentation of different types of non-digestible carbohydrates in the large intestine of swine, shedding light on their potential roles and contributions to swine nutrition and health.
Time Spent on Private Tutoring and Sleep Patterns of Chinese Adolescents: Evidence from a National Panel Survey
Authors: Zhang Yueyun
Published: July 2023

Objective: In recent decades, there has been a marked increase in private tutoring and a decline in sleep health among adolescents. This study aimed to outline the association between time spent on private tutoring and sleep patterns of Chinese adolescents. Methods: Participants were from a nationwide two-wave panel survey. We performed OLS regressions of sleep duration at wave 2 and Poisson regressions of sleep problems at wave 2 on tutoring time at wave 2, adjusting for baseline sleep duration and other covariates. Tutoring time was assessed by three items: daily hours of tutoring, daily hours of tutoring on weekdays, and daily hours of tutoring on weekends. Results: Overall, more time spent on private tutoring was associated with shorter sleep duration and more sleep problems. Furthermore, both weekday and weekend tutoring can have a positive effect on the number of sleep problems. With regard to sleep duration, weekend tutoring time played a negative role, while the effect of weekday tutoring time was negligible. Conclusion: In the Chinese context, which is characterized by intense academic competition, participation in private tutoring plays a significant role in shaping students’ sleep duration and sleep problems. To improve the sleep health of adolescents, the time spent on private tutoring should be carefully monitored and regulated.
A New Single-Cell Hybrid Inductor-Capacitor DC-DC Converter for Ultra-High Voltage Gain in Renewable Energy Applications
Authors: Algamluoli Ammar Falah , Wu Xiaohua
Published: July 2023

In this paper, a new single-cell hybrid switched inductor DC-DC converter is proposed to demonstrate the verification of ultra-high voltage gain in renewable energy applications (REA). The modification involves adding a single cell of an inductor with a diode and double capacitor to increase voltage transfer gain. Additionally, this modification helps prevent the input current from becoming zero, pulsating at very low duty cycles. The single cell of the hybrid inductor is interleaved with the main switch to reduce current stress when the capacitor of the single-cell inductor charge becomes zero. Moreover, the addition of a modified hybrid switch inductor with a capacitor, operating in dual boosting mode with a single switch, allows the converter to achieve ultra-high voltage gain. The proposed converter offers several advantages, including ultra-high voltage gain, high efficiency, low voltage stress on power MOSFETs, diodes, inductors, and capacitors, as well as low switching and conduction losses. Furthermore, the proposed converter utilizes transformerless and non-coupled inductors. Mathematical equations have been derived for the discontinuous conduction mode (DCM) and continuous conduction mode (CCM) and implemented using Matlab Simulink software to validate the results. In addition, a dual PI controller is designed for the proposed converter to verify the fixed output voltage. Experimental results have also been obtained for a 200 W prototype, with the input voltage varying between 20 V and 40 V, and an output voltage of 200 V at an efficiency of 96.5%.
Effective Non-Stationary Clutter Suppression Method via Elevation Oblique Subspace Projection for Moving Targets Detection with a Space-Based Surveillance Radar
Authors: Wang Xiaofeng , Ruan Yaduan , Zhang Xinggan
Published: July 2023

The clutter becomes non-stationary for a space-based surveillance radar (SBSR), which is harmful for the moving targets detection due to the earth’s rotation. The non-stationarity will degrade the accuracy of clutter covariance matrix (CCM) estimation and increase the clutter degree of freedom (DOF), thereby degrading the performance of clutter suppression. To solve this problem, this paper proposes a novel non-stationary clutter suppression method using an elevation oblique subspace projection method. After analyzing the range ambiguity and non-stationarity of the clutter, the proposed method utilized the oblique projection matrix to project the signal onto the subspace spanned by the near-range and far-range clutter components along the subspace spanned by the main lobe clutter component. Then, the projected signal was used to estimate the elevation covariance matrix and calculate the optimal weight vector for the elevation adaptive filter. The proposed method can suppress the non-stationary clutter effectively with a higher improvement factor (IF) and a narrower main lobe width. Finally, the simulation results were given to verify the correctness and effectiveness of the proposed method.
Using Non-Standard Finite Difference Scheme to Study Classical and Fractional Order SEIVR Model
Authors: Din Rahim Ud , Khan Khalid Ali , Aloqaily Ahmad , Mlaiki Nabil , Alrabaiah Hussam
Published: July 2023

In this study, we considered a model for novel COVID-19 consisting on five classes, namely S, susceptible; E, exposed; I, infected; V, vaccinated; and R, recovered. We derived the expression for the basic reproductive rate R0 and studied disease-free and endemic equilibrium as well as local and global stability. In addition, we extended the nonstandard finite difference scheme to simulate our model using some real data. Moreover, keeping in mind the importance of fractional order derivatives, we also attempted to extend our numerical results for the fractional order model. In this regard, we considered the proposed model under the concept of a fractional order derivative using the Caputo concept. We extended the nonstandard finite difference scheme for fractional order and simulated our results. Moreover, we also compared the numerical scheme with the traditional RK4 both in CPU time as well as graphically. Our results have close resemblance to those of the RK4 method. Also, in the case of the infected class, we compared our simulated results with the real data.
Stability of Time Series Models Based on Fractional-Order Weakening Buffer Operators
Authors: Li Chong , Yang Yingjie , Zhu Xinping
Published: July 2023

Different weakening buffer operators in a time-series model analysis usually result in different model sensitivities, which sometimes affect the effectiveness of relevant operator-based methods. In this paper, the stability of two classic fractional-order weakening buffer operator-based series models is studied; then, a new data preprocessing method based on a novel fractional-order bidirectional weakening buffer operator is provided, whose effect in improving the model’s stability is tested and utilized in prediction problems. Practical examples are employed to demonstrate the efficiency of the proposed method in improving the model’s stability in noise scenarios. The comparison indicates that the proposed method overcomes the disadvantage of many weakening buffer operators in the subjectively biased weighting of the new or old information in forecasting. These expand the application of the proposed method in time series analysis.
IMVTS: A Detection Model for Multi-Varieties of Famous Tea Sprouts Based on Deep Learning
Authors: Zhao Runmao , Liao Cong , Yu Taojie , Chen Jianneng , Li Yatao , Lin Guichao , Huan Xiaolong , Wang Zhiming
Published: July 2023

The recognition of fresh tea leaf sprouts is one of the difficulties in the realization of the automated picking of fresh tea leaves. At present, the research on the detection of fresh tea leaf sprouts is based on a single variety of tea leaves for a specific period or specific place, which has no advantage for the spread, promotion, and application of the methods. To address this problem, an identification of multiple varieties of tea sprouts (IMVTS) model was proposed. First, images of three different varieties of tea (ZhongCha108 (ZC108), ZhongHuangYiHao (ZH), ZiJuan (ZJ)) were obtained, and the multiple varieties of tea (MVT) dataset for training and validating models was created. In addition, the detection effects of adding a convolutional block attention module (CBAM) or efficient channel attention (ECA) module to YOLO v7 were compared. In the detection of the MVT dataset, YOLO v7+ECA and YOLO v7+CBAM showed a higher mean average precision (mAP) than YOLO v7, with 98.82% and 98.80%, respectively. Notably, the IMVTS model had the highest AP for ZC108, ZH, and ZJ compared with the two other models, with 99.87%, 96.97%, and 99.64%, respectively. Therefore, the IMVTS model was proposed on the basic framework of the ECA and YOLO v7. To further illustrate the superiority of the model, this study also conducted a comparison test between the IMVTS model and the mainstream target detection models (YOLO v3, YOLO v5, FASTER-RCNN, and SSD) and the IMVTS model on the VOC dataset, and it is clear from the test results that the mAP of the IMVTS model is ahead of the remaining models. Concisely, the detection accuracy of the IMVTS model can meet the engineering requirements for the automatic harvesting of autumn fresh famous tea leaves, which provides a basis for the future design of detection networks for other varieties of autumn tea sprouts.
Effects of Sulfate on the Physiology, Biochemistry, and Activity of Group 1 Sulfate Transporters in Seedlings of Brassica pekinensis
Authors: Prajapati Dharmendra , Patani Anil , Patel Margi , Ali Daoud , Alarifi Saud , Yadav Virendra Kumar , Tank Jigna , Patel Ashish
Published: July 2023

It is well known that some plants have the capability of taking up sulfur as a nutrient from the atmosphere through foliar absorption and can survive well in polluted environments. In order to observe the effects of the relationship between atmospheric hydrogen sulfide (H2S) deposition and soil sulfur nutrition, the current study used Brassica pekinensis as a model plant. The objective in conducting this study was to understand the regulatory mechanisms engaged in the uptake and assimilation of sulfate (SO42−) in plants by studying the modulation of transcription levels of sulfate transporter genes (STGs) (Sultr1;1 and Sultr1;2), changes in growth physiology, and the potential of roots to uptake the SO42− when allowed to grow in the presence or absence of SO42− in a hydroponic nutrient solution. Changes in growth, physico-chemical parameters, and gene expression levels of Group 1 STGs were observed when sulfur-treated and non-treated plants were exposed to phytotoxic H2S levels in the air. Sulfur deficiency enhanced nitrate and free amino acid (FAA) concentrations in the shoot and root regions of the plant. However, there was a significant decrease in the biomass, shoot/root ratio (SRR), chlorophyll content, and thiol content, with p-values < 0.01. This, in turn, increased the sulfur-uptake capacity of plants from the atmosphere through foliar absorption. When the sulfur-uptake capacity of plants increased, there was an increase in the expression level of Group 1 sulfate transporter genes (Sultr1;1 and Sultr1;2), which regulate sulfur transportation through roots. The growth, physico-chemical characteristics, and level of gene expression of Group 1 STGs were unaffected by the availability of excess sulfur in the atmosphere of up to 0.3 μL l−1.
Synergistic Effect of Substance P with Insulin and Insulin-Like Growth Factor-I on Epithelial Migration of the Transformed Human Corneal Epithelial Cells (SV-40)
Authors: Tran Phat , Kopel Jonathan , Reid Ted W.
Published: July 2023

On the cornea, daily epithelial resurfacing is a critical process to prevent the loss of normal function, ocular morbidity, corneal structure, and vision loss. There are several components to wound healing, including cellular attachment, migration, and growth. To develop a treatment for corneal epithelial healing, we studied the effect of substance P (SP) on corneal epithelial cell migration using a cell culture system of either transformed human corneal epithelial cells (SV-40), or rabbit corneal epithelial cells (SIRC). We investigated the effect of SP with insulin and insulin-like growth factor-I (IGF-1). We found that SP is synergistic with insulin and IGF-1 on the stimulation of transformed human corneal epithelial migration in a cell culture system, as well as in rabbit SIRC cells. The addition of either SP, insulin, or IGF-1 alone did not greatly affect epithelial migration, while the combination of SP and insulin or SP and IGF-1 markedly stimulated epithelial migration in a dose-dependent manner. The synergistic effects of SP with insulin and SP with IGF-1 were inhibited by the addition of the SP-specific inhibitor (CP96345). However, the effect of insulin and IGF-1 alone were not inhibited by the NK-1-specific inhibitor (CP96345). Our results are consistent with the need for the involvement of the neuropeptide SP in corneal epithelial wound healing of diabetic corneas where nerve-ending dropout occurs. Additionally, almost identical results were obtained with human and rabbit corneal epithelial cells. These results suggest that the maintenance of the normal integrity of the corneal epithelium might be regulated by both humoral and neural factors.
Semi-Automatic GUI Platform to Characterize Brain Development in Preterm Children Using Ultrasound Images
Authors: Rabanaque David , Regalado Maria , Benítez Raul , Rabanaque Sonia , Agut Thais , Carreras Nuria , Mata Christian
Published: July 2023

The third trimester of pregnancy is the most critical period for human brain development, during which significant changes occur in the morphology of the brain. The development of sulci and gyri allows for a considerable increase in the brain surface. In preterm newborns, these changes occur in an extrauterine environment that may cause a disruption of the normal brain maturation process. We hypothesize that a normalized atlas of brain maturation with cerebral ultrasound images from birth to term equivalent age will help clinicians assess these changes. This work proposes a semi-automatic Graphical User Interface (GUI) platform for segmenting the main cerebral sulci in the clinical setting from ultrasound images. This platform has been obtained from images of a cerebral ultrasound neonatal database images provided by two clinical researchers from the Hospital Sant Joan de Déu in Barcelona, Spain. The primary objective is to provide a user-friendly design platform for clinicians for running and visualizing an atlas of images validated by medical experts. This GUI offers different segmentation approaches and pre-processing tools and is user-friendly and designed for running, visualizing images, and segmenting the principal sulci. The presented results are discussed in detail in this paper, providing an exhaustive analysis of the proposed approach’s effectiveness.
Four Novel Species and Two New Records of Boletes from India
Authors: Das Kanad , Ghosh Aniket , Chakraborty Dyutiparna , Datta Sudeshna , Bera Ishika , Layola MR Ranjith , Banu Farheen , Vizzini Alfredo , Wisitrassameewong Komsit
Published: July 2023

Repeated macrofungal explorations, followed by thorough examination of species through morphology and molecular phylogeny, have made it clear that European and American names of wild mushrooms were inadvertently misapplied quite often to Asian lookalikes by mycologists/taxonomists in the past. Therefore, in order to reveal this mushroom treasure, in recent years, taxonomical research on wild mushrooms has been intensified in Asian countries, including India, by undertaking a combined approach of morpho-taxonomy and multigene molecular phylogeny. Boletoid mushrooms (Boletaceae) are no exception. While working on boletoid mushrooms of the Indian Himalayas, authors recently came across six interesting species of boletoid mushrooms. In the present communication, four novel species, namely Leccinellum binderi, Cyanoboletus paurianus, Xerocomus uttarakhandae, and Xerocomellus himalayanus, are established based on morphology and molecular phylogenetic estimations. Moreover, Cyanoboletus macroporus and Xerocomus fraternus are also reported here for the first time in India.
Is Continuous Positive Airway Pressure a Valid Alternative to Sildenafil in Treating Sexual Dysfunction among OSA Patients? A Systematic Review and Meta-Analysis
Authors: Stilo Giovanna , Vicini Claudio , Pollicina Isabella , Maniaci Antonino , Lechien Jérôme René , Calvo-Henríquez Christian , Yáñez Miguel Mayo , Iannella Giannicola , Pace Annalisa , Cammaroto Giovanni , Meccariello Giuseppe , Cannavicci Angelo , Moffa Antonio , Casale Manuele , La Mantia Ignazio
Published: July 2023

Background and Objectives: This study aimed to assess the comparative effectiveness of continuous positive airway pressure (CPAP) therapy and sildenafil pharmacological therapy in improving sexual function among patients with obstructive sleep apnea (OSA) and erectile dysfunction (ED). Materials and methods: Population: Patients affected by OSA and ED; Intervention: CPAP therapy vs. Comparison: Sildenafil pharmacological therapy; Outcomes: Improvement in erectile function, as measured by the International Index of Erectile Function 5 (IIEF-5) scoring system; Time: A systematic review of the literature from the past 20 years; Study Design: Observational studies comparing erectile function improvements after OSA treatment. Results: A total of eight papers were included in the qualitative summary, involving four hundred fifty-seven patients with ED and OSA. Erectile function improvements were observed in both treatment groups. After sildenafil and CPAP treatment, the mean IIEF-5 domain scores were 37.7 and 27.3, respectively (p < 0.001). Sildenafil 100 mg demonstrated a higher therapeutic impact compared to CPAP treatment. Conclusions: CPAP therapy significantly improved sexual parameters in most studies for OSA patients with ED. The findings suggest that CPAP therapy effectively alleviates erectile dysfunction symptoms, resulting in improved sexual performance in OSA patients. The comparison of the two treatments indicates that sildenafil has a more substantial therapeutic impact on erectile function than CPAP therapy; however, a combined treatment will provide a cumulative effect.
Accuracy of Serological Screening for the Diagnosis of Celiac Disease in Type 1 Diabetes Children
Authors: Girard Chloé , De Percin Aurélie , Morin Carole , Talvard Maeva , Fortenfant Françoise , Congy-Jolivet Nicolas , Le Tallec Claire , Olives Jean-Pierre , Mas Emmanuel
Published: July 2023

Background and Objectives: Patients with type 1 diabetes (T1D) are considered at high-risk for developing celiac disease (CD). The purpose of our study was to determine the prevalence of CD among children who were followed in our unit for T1D using the latest ESPGHAN guidelines, and avoiding intestinal biopsies in some of the children. Materials and Methods: We performed a prospective monocentric study, which included 663 T1D children between June 2014 and June 2016. We considered CD according to serological (tissue transglutaminase (TGAs) and endomysium antibodies) results. Children were included either at the time of T1D diagnosis or during their follow up. We looked for clinical and biochemical signs of CD, and for T1D characteristics. Results: The children’s ages ranged from 11 months to 18 years. CD was confirmed in 32 out of 663 patients with T1D, with a prevalence of 4.8%. CD was excluded in 619 children and remained uncertain for 12 children, who had positive TGAs without the required criteria. We found that 95% of T1D children express HLA-DQ2 and/or -DQ8, which was 2.4 times higher than in the general population. Conclusions: An intestinal biopsy could be avoided to confirm CD in the majority of T1D children. Silent forms of CD are frequent and screening is recommended for all patients. Importantly, repeated TGA assessment is required in HLA genetically predisposed T1D patients, while it is unnecessary in the 5% who are HLA-DQ2 and -DQ8 negative.
Exploring the Mechanisms behind the Anti-Tumoral Effects of Model C-Scorpionate Complexes
Authors: Silva Pedro M. G. , Pinheiro Pedro F. , Camões Sérgio P. , Ribeiro Ana P. C. , Martins Luísa M. D. R. S. , Miranda Joana P. G. , Justino Gonçalo C.
Published: July 2023

The growing worldwide cancer incidence, coupled to the increasing occurrence of multidrug cancer resistance, requires a continuous effort towards the identification of new leads for cancer management. In this work, two C-scorpionate complexes, [FeCl2(κ3-Tpm)] (1) and [Co(κ3-TpmOH)2](NO3)2 (2), (Tpm = hydrotris(pyrazol-1-yl)methane and TpmOH = 2,2,2-tris(pyrazol-1-yl)ethanol), were studied as potential scaffolds for future anticancer drug development. Their cytotoxicity and cell migration inhibitory activity were analyzed, and an untargeted metabolomics approach was employed to elucidate the biological processes significantly affected by these two complexes, using two tumoral cell lines (B16 and HCT116) and a non-tumoral cell line (HaCaT). While [FeCl2(κ3-Tpm)] did not display a significant cytotoxicity, [Co(κ3-TpmOH)2](NO3)2 was particularly cytotoxic against the HCT116 cell line. While [Co(κ3-TpmOH)2](NO3)2 significantly inhibited cell migration in all tested cell lines, [FeCl2(κ3-Tpm)] displayed a mixed activity. From a metabolomics perspective, exposure to [FeCl2(κ3-Tpm)] was associated with changes in various metabolic pathways involving tyrosine, where iron-dependent enzymes are particularly relevant. On the other hand, [Co(κ3-TpmOH)2](NO3)2 was associated with dysregulation of cell adhesion and membrane structural pathways, suggesting that its antiproliferative and anti-migration properties could be due to changes in the overall cellular adhesion mechanisms.
Influence of the Emulsifier Sodium Caseinate–Xanthan Gum Complex on Emulsions: Stability and Digestive Properties
Authors: Huang Huan , Tian Yan , Bai Xinpeng , Cao Yumiao , Fu Zihuan
Published: July 2023

In this study, virgin coconut oil (VCO) nanoemulsions were prepared by ultrasonication using a sodium caseinate (SC) and xanthan gum (XG) complex as an emulsifier. The stability and digestion characteristics of SC/XG–VCO emulsions formed by co-adsorption and SC–VCO–XG emulsions formed by layer adsorption were compared. The stability of the two emulsions was studied under different pH, ionic strength, heat treatment, freeze–thaw cycles, and storage conditions, and the droplet size and zeta potential were used as indicators to assess the stability. In addition, the stability of oxidation and the digestive properties of both emulsions were studied. It was found that the SC–VCO–XG emulsions had better environmental stability, oxidative stability, storage stability, and digestibility compared to SC/XG–VCO emulsions. This study has shown that the formation method of protein–polysaccharide stabilized emulsions has an impact on the stability and digestibility properties of the emulsions, and that the emulsion carriers constructed by layer adsorption are more suitable for subsequent industrial production and development.
Synthesis, Structure, and Characterization of Thiacalix[4]-2,8-thianthrene
Authors: Ueda Masafumi , Isozaki Moe , Mazaki Yasuhiro
Published: July 2023

Sulfur-containing macrocycles have attracted substantial interest because they exhibit unique characteristics due to their polygonal ring-shaped skeleton. In this study, a thianthrene-based cyclic tetramer with the sulfur linker, thiacalix[4]-2,8-thianthrene (TC[4]TT), was successfully prepared from a cyclo-p-phenylenesulfide derivative using acid-induced intramolecular condensation. Single crystal X-ray diffraction revealed that TC[4]TT adopts an alternative octagonal form recessed to the inner side. Its internal cavity included small solvents, such as chloroform and carbon disulfide. Due to its polygonal geometry, TC[4]TT laminated in a honeycomb-like pattern with a porous channel. Furthermore, TC[4]TT showed fluorescence and phosphorescence emission in a CH2Cl2 solution at ambient and liquid nitrogen temperatures. Both emission bands were slightly redshifted compared with those of the reference compounds (di(thanthren-2-yl)sulfane (TT2S) and thianthrene (TT)). This work describes a sulfur-containing thiacalixheterocycle-based macrocyclic system with intriguing supramolecular chemistry based on molecular tiling and photophysical properties in solution.
Anti-Inflammatory and Antiatopic Effects of Rorippa cantoniensis (Lour.) Ohwi in RAW 264.7 and HaCaT Cells
Authors: Kim Min-Jin , Hwang Buyng Su , Hwang Yong , Jeong Yong Tae , Jeong Dae Won , Oh Young Taek
Published: July 2023

This study evaluated the effects of Rorippa cantoniensis (Lour.) ohwi extract (RCE) on factors associated with inflammation-related skin lesions in RAW 264.7 and HaCaT cells. RCE inhibited the levels of proinflammatory mediators and cytokines such as nitric oxide (NO), prostaglandin E2 (PGE2), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. In addition, RCE significantly inhibited the expression of chemokines and cytokines such as MDC/CCL22, TARC/CCL17, RANTES/CCL5, CTSS, IL-6, IL-1β, and TNF-α in HaCaT cells costimulated by TNF-α and interferon (IFN)-γ in a concentration-dependent manner. These results suggest that RCE attenuated the TNF-α- and IFN-γ-induced release of proinflammatory chemokines and cytokines probably by suppressing the activation of MAPK (JNK and p38), NF-κB, and STAT1 signaling. Moreover, RCE significantly increased the expression of skin components such as hyaluronic acid and aquaporin, which play important roles in the physical and chemical barriers of the skin. These results suggest that RCE has significant anti-inflammatory and antiatopic activities, which may be beneficial for the topical treatment of inflammatory skin disorders.
Bodipy Dimer for Enhancing Triplet-Triplet Annihilation Upconversion Performance
Authors: Gao Min , Zeng Le , Jiang Linhan , Zhang Mingyu , Chen Yong , Huang Ling
Published: July 2023

Triplet-triplet annihilation upconversion (TTA-UC) has considerable potential for emerging applications in bioimaging, optogenetics, photoredox catalysis, solar energy harvesting, etc. Fluoroboron dipyrrole (Bodipy) dyes are an essential type of annihilator in TTA-UC. However, conventional Bodipy dyes generally have large molar extinction coefficients and small Stokes shifts (<20 nm), subjecting them to severe internal filtration effects at high concentrations, and resulting in low upconversion quantum efficiency of TTA-UC systems using Bodipy dyes as annihilators. In this study, a Bodipy dimer (B-2) with large Stokes shifts was synthesized using the strategy of dimerization of an already reported Bodipy annihilator (B-1). Photophysical characterization and theoretical chemical analysis showed that both B-1 and B-2 can couple with the red light-activated photosensitizer PdTPBP to fulfill TTA-UC; however, the higher fluorescence quantum yield of B-2 resulted in a higher upconversion efficiency (ηUC) for PdTPBP/B-2 (10.7%) than for PdTPBP/B-1 (4.0%). This study proposes a new strategy to expand Bodipy Stokes shifts and improve TTA-UC performance, which can facilitate the application of TTA-UC in photonics and biophotonics.
A Case of De Novo Positional Complex Sleep Apnea Syndrome (CompSAS)
Authors: Khalifa Abdelkarim , Spielmanns Marc
Published: July 2023

Obstructive sleep apnea (OSA) is well known to often improve with non-supine positioning as opposed to supine positioning. Emerging research supports a role for sleep position management in patients with central sleep apnea (CSA) as well. We report a case of de novo complex sleep apnea syndrome (CompSAS) in a 78-year-old female, who presented after a car accident due to unclear syncope. Diagnostic polysomnography (PSG) showed moderate OSA. A CompSAS developed under automatic positive airway pressure (APAP), while 4 years of downloaded data showed good adherence. No significant benefit was reported under adaptive servo ventilation (ASV) and BiPAP-ST, while a reduction in CSA in the non-supine position was noticed. Oxygen and sleep positional therapy (SPT) were considered, resulting in a significant improvement in CSA and sleep quality. Further research on the prevalence of positional CSA is needed.
High-Precision Calibration of a Monocular-Vision-Guided Handheld Line-Structured-Light Measurement System
Authors: Zhou Jingbo , Ji Zhaohui , Li Yuehua , Liu Xiaohong , Yao Wenhao , Qin Yafang
Published: July 2023

Due to the advantages of simple construction, easy application and good environmental suitability, handheld structured-light measurement systems have broad application prospects in 3D measurements. Here, a monocular-vision-guided line-structured-light measurement system is developed, and the posture of the handheld device can be obtained via a specifically designed target attached to it. No more marker points need to be adhered onto the object under inspection. The key for the system calibration is to obtain the coordinate transformation matrix from the sensor to the featured target coordinate system. The mathematical model of the system is first established. Then, an improved multi-view calibration method is proposed, where a selection process for the image pairs is conducted for accuracy improvement. With this method, the maximum relative error of the measured stair heights can be reduced from 0.48% to 0.16%. The measurement results for the specific parts further verified the effectiveness of the proposed system and the calibration method.