bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Postoperative Coronal Leg Alignment in Customized Individually Made and Conventional Total Knee Arthroplasty
Authors: Wunderlich Felix , Azad Maheen , Westphal Ruben , Klonschinski Thomas , Belikan Patrick , Drees Philipp , Eckhard Lukas
Published: June 2021

Neutral coronal leg alignment is known to be important for postoperative outcome in total knee arthroplasty (TKA). Customized individually made implants (CIM) instrumented with patient-specific cutting guides are an innovation aiming to increase the precision and reliability of implant positioning and reconstruction of leg alignment. We aimed to compare reconstruction of the hip–knee–ankle angle (HKA) of the novel CIM system iTotal™ CR G2 (ConforMIS Inc.) to a matched cohort of the off-the-shelf (OTS) knee replacement system Vanguard™ CR (Zimmer Biomet). Retrospective analysis of postoperative coronal full-leg weight-bearing radiographs of 562 TKA (283 CIM TKA, 279 OTS TKA) was conducted. Via a medical planning software, HKA and rotation of the leg were measured in postoperative radiographs. HKA was then adjusted for rotational error, and 180° ± 3° varus/valgus was defined as the target zone HKA. Corrected postoperative HKA in the CIM group was 179.0° ± 2.8° and 179.2° ± 3.1° in the OTS group (p = 0.34). The rate of outliers, outside of the ±3° target zone, was equal in both groups (32.9%). Our analysis showed that TKA using patient-specific cutting guides and implants and OTS TKA implanted with conventional instrumentation resulted in equally satisfying restoration of the coronal leg alignment with less scattering in the CIM group.
Incidence and Treatment Outcome of Rhinosinusitis before Kidney Transplantation: A Retrospective Cohort Study
Authors: Oh Jin Seok , Kim Min Soo , Kim Sung Hee , Kim Ji Heui
Published: June 2021

Background: The use of immunosuppressants after transplantation can aggravate sinus infections. Although kidney transplantation (KT) recipients are administered strong immunosuppressant therapy, there is few consensus or reports on incidence and treatment of rhinosinusitis before KT. This study was undertaken to analyze the results of a cohort of KT recipients that underwent sinonasal evaluation before KT. Methods: Observational retrospective cohort data were analyzed from adults who underwent a KT between January 2015 and December 2018. In total, 966 patients were screened by clinical history, nasal endoscopy, and plain X-ray before KT. Results: A total of 86 patients (8.9%) were diagnosed with rhinosinusitis. Twenty-three of the eighty-six patients (26.7%) who underwent plain X-ray on second follow up were successfully treated with primary and secondary antibiotics, saline irrigation, and INS. From the remaining 63 patients who underwent additional CT on second follow up, 43 patients were treated with primary or secondary antibiotics and 20 patients (10 with chronic rhinosinusitis and 10 with fungal ball) were treated with endoscopic sinus surgery. There were no serious complications affecting patient mortality after KT. Conclusion: We report that 8.9% of patients showed abnormal findings in sinonasal evaluation before KT. Although most patients did not require surgery, surgery is recommended for active rhinosinusitis, which does not respond to medication, and for fungal rhinosinusitis to prevent postoperative sinonasal infection.
The Functionality of UDP-Glucuronosyltransferase Genetic Variants and their Association with Drug Responses and Human Diseases
Authors: Jarrar Yazun , Lee Su-Jun
Published: June 2021

UDP-glucuronosyltransferases (UGTs) are phase II drug-metabolizing enzymes that metabolize endogenous fatty acids such as arachidonic acid metabolites, as well as many prescription drugs, such as opioids, antiepileptics, and antiviral drugs. The UGT1A and 2B genes are highly polymorphic, and their genetic variants may affect the pharmacokinetics and hence the responses of many drugs and fatty acids. This study collected data and updated the current view of the molecular functionality of genetic variants on UGT genes that impact drug responses and the susceptibility to human diseases. The functional information of UGT genetic variants with clinical associations are essential to understand the inter-individual variation in drug responses and susceptibility to toxicity.
Construction Cash Flow Risk Index
Authors: Mahmoud Hasan , Ahmed Vian , Beheiry Salwa
Published: June 2021

As investment increases in capital projects, financial risks increase, and cash flow prediction and control become more paramount. Higher risks could hinder project performance and increase the chances of failure in multiple aspects of a project. While there are models that aim to assess and forecast risks in the construction industry, none present a technique to include the impact of risks on a project’s cash flow. Therefore, cash flow forecasts tend to exceed the actual cash flow of a project due to inaccurate risk assessment. Thus, this paper presents the Cash Flow Risk Index (CFRI) development process quantifying the impact of risks on a project’s cash flow from an owner’s perspective. To that end, the study explored the literature to identify the risk factors that might impact a construction projects’ cash flow and uncovered 44 factors. The study also validated and consolidated these factors to build a CFRI via a Delphi exercise, which reduced the factors from 44 to 36. In further iterations, the 36 factors were also shared with 32 construction industry professionals to rate their relative importance on a five-point Likert scale, from which relative importance index and weights were obtained. As a result, the CFRI was developed to measure the impact of different risk factors on a typical construction project’s cash flow.
Fluorescence Lifetime Changes Induced by Laser Irradiation: A Preclinical Study towards the Evaluation of Retinal Metabolic States
Authors: Sonntag Svenja Rebecca , Seifert Eric , Hamann Maximilian , Lewke Britta , Theisen-Kunde Dirk , Grisanti Salvatore , Brinkmann Ralf , Miura Yoko
Published: June 2021

Fluorescence Lifetime (FLT) of intrinsic fluorophores may alter under the change in metabolic state. In this study, the FLT of rabbit retina was investigated in vivo after laser irradiation using fluorescence lifetime imaging ophthalmoscopy (FLIO). The retina of the Chinchilla bastard rabbits was irradiated with a 514 nm diode laser. FLIO, fundus photography, and optical coherence tomography (OCT) were conducted 30 min and 1 to 3 weeks after treatment. After strong coagulation, the FLT at laser spots was significantly elongated immediately after irradiation, conversely shortened after more than a week. Histological examination showed eosinophilic substance and melanin clumping in subretinal space at the coagulation spots older than one week. The FLT was also elongated right around the coagulation spots, which corresponded to the discontinuous ellipsoid zone (EZ) on OCT. This EZ change was recovered after one week, and the FLT became the same level as the surroundings. In addition, there was a region around the laser spot where the FLT was temporarily shorter than the surrounding area. When weak pulse energy was applied to selectively destroy only the RPE, a shortening of the FLT was observed immediately around the laser spot within one week after irradiation. FLIO could serve as a tool to evaluate the structural and metabolic response of the retina to laser treatments.
Strong Shift to ATR-Dependent Regulation of the G2-Checkpoint after Exposure to High-LET Radiation
Authors: Mladenova Veronika , Mladenov Emil , Scholz Michael , Stuschke Martin , Iliakis George
Published: June 2021

The utilization of high linear-energy-transfer (LET) ionizing radiation (IR) modalities is rapidly growing worldwide, causing excitement but also raising concerns, because our understanding of their biological effects is incomplete. Charged particles such as protons and heavy ions have increasing potential in cancer therapy, due to their advantageous physical properties over X-rays (photons), but are also present in the space environment, adding to the health risks of space missions. Therapy improvements and the protection of humans during space travel will benefit from a better understanding of the mechanisms underpinning the biological effects of high-LET IR. There is evidence that high-LET IR induces DNA double-strand breaks (DSBs) of increasing complexity, causing enhanced cell killing, owing, at least partly, to the frequent engagement of a low-fidelity DSB-repair pathway: alternative end-joining (alt-EJ), which is known to frequently induce severe structural chromosomal abnormalities (SCAs). Here, we evaluate the radiosensitivity of A549 lung adenocarcinoma cells to X-rays, α-particles and 56Fe ions, as well as of HCT116 colorectal cancer cells to X-rays and α-particles. We observe the expected increase in cell killing following high-LET irradiation that correlates with the increased formation of SCAs as detected by mFISH. Furthermore, we report that cells exposed to low doses of α-particles and 56Fe ions show an enhanced G2-checkpoint response which is mainly regulated by ATR, rather than the coordinated ATM/ATR-dependent regulation observed after exposure to low doses of X-rays. These observations advance our understanding of the mechanisms underpinning high-LET IR effects, and suggest the potential utility for ATR inhibitors in high-LET radiation therapy.
Surgery for Coagulopathy-Related Intracerebral Hemorrhage: Craniotomy vs. Minimally Invasive Neurosurgery
Authors: Liu Yen-Bo , Kuo Lu-Ting , Chen Chih-Hao , Kung Woon-Man , Tsai Hsin-Hsi , Chou Sheng-Chieh , Yang Shih-Hung , Wang Kuo-Chuan , Lai Dar-Ming , Huang Abel Po-Hao
Published: June 2021

Coagulopathy-related intracerebral hemorrhage (ICH) is life-threatening. Recent studies have shown promising results with minimally invasive neurosurgery (MIN) in the reduction of mortality and improvement of functional outcomes, but no published data have recorded the safety and efficacy of MIN for coagulopathy-related ICH. Seventy-five coagulopathy-related ICH patients were retrospectively reviewed to compare the surgical outcomes between craniotomy (n = 52) and MIN (n = 23). Postoperative rebleeding rates, morbidity rates, and mortality at 1 month were analyzed. Postoperative Glasgow Outcome Scale Extended (GOSE) and modified Rankin Scale (mRS) scores at 1 year were assessed for functional outcomes. Morbidity, mortality, and rebleeding rates were all lower in the MIN group than the craniotomy group (8.70% vs. 30.77%, 8.70% vs. 19.23%, and 4.35% vs. 23.08%, respectively). The 1-year GOSE score was significantly higher in the MIN group than the craniotomy group (3.96 ± 1.55 vs. 3.10 ± 1.59, p = 0.027). Multivariable logistic regression analysis also revealed that MIN contributed to improved GOSE (estimate: 0.99650, p = 0.0148) and mRS scores (estimate: −0.72849, p = 0.0427) at 1 year. MIN, with low complication rates and improved long-term functional outcome, is feasible and favorable for coagulopathy-related ICH. This promising result should be validated in a large-scale prospective study.
Development of a System for Additive Manufacturing of Ceramic Matrix Composite Structures Using Laser Technology
Authors: Polenz Stefan , Kunz Willy , Braun Benjamin , Franke Andrea , López Elena , Brückner Frank , Leyens Christoph
Published: June 2021

Ceramic matrix composites (CMCs) are refractory ceramic materials with damage-tolerant behavior. Coming from the space industry, this class of materials is increasingly being used in other applications, such as automotive construction for high-performance brake discs, furnace technology, heat coatings for pipe systems and landing flaps on reusable rocket sections. In order to produce CMC faster and more cost-efficiently for the increasing demand, a new additive manufacturing process is being tested, which in the future should also be able to realize material joints and higher component wall thicknesses than conventional processes. The main features of the process are as follows. A ceramic fiber bundle is de-sized and infiltrated with ceramic suspension. The bundle infiltrated with matrix material is dried and then applied to a body form. During application, the matrix material is melted by laser radiation without damaging the fiber material. For the initial validation of the material system, samples are pressed and analyzed for their absorption properties using integrating sphere measurement. With the results, a suitable processing laser is selected, and initial melting tests of the matrix system are carried out. After the first validation of the process, a test system is set up, and the first test specimens are produced to determine the material parameters.
Framework Materials for Full-Arch Implant-Supported Rehabilitations: A Systematic Review of Clinical Studies
Authors: Delucchi Francesca , De Giovanni Emanuele , Pesce Paolo , Bagnasco Francesco , Pera Francesco , Baldi Domenico , Menini Maria
Published: June 2021

The purpose of this systematic review was to investigate the clinical outcomes of frameworks made of different materials in patients with implant-supported full-arch prostheses. A literature search was conducted on MEDLINE, Scopus and Cochrane Library, until the 1st of March 2021, with the following search terms: framework or substructure combined with “dental implants”. The outcomes evaluated were: implant and prosthesis survival, bone resorption, biological and technical complications. The Cochrane Handbook for Systematic Reviews of Interventions was employed to assess the risk of bias in randomized clinical trials. The Newcastle–Ottawa quality assessment scale was used for non-randomized studies. In total, 924 records were evaluated for title and abstract, and 11 studies were included in the review: 4 clinical randomized trials and 7 cohort studies. The framework materials investigated were: gold alloy, titanium, silver-palladium alloy, zirconia and polymers including acrylic resin and carbon-fiber-reinforced composites. High implant and prosthetic cumulative survival rates were recorded by all included studies. Various materials and different fabrication techniques are now available as alternatives to traditional cast metal frameworks, for full-arch implant-supported rehabilitations. Further long-term studies are needed to validate the use of these materials and clarify their specific clinical indications and manufacturing protocols to optimize their clinical outcomes.
Structural Factors Inducing Cracking of Brass Fittings
Authors: Kunčická Lenka , Jambor Michal , Weiser Adam , Dvořák Jiří
Published: June 2021

Cu–Zn–Pb brasses are popular materials, from which numerous industrially and commercially used components are fabricated. These alloys are typically subjected to multiple-step processing—involving casting, extrusion, hot forming, and machining—which can introduce various defects to the final product. The present study focuses on the detailed characterization of the structure of a brass fitting—i.e., a pre-shaped medical gas valve, produced by hot die forging—and attempts to assess the factors beyond local cracking occurring during processing. The analyses involved characterization of plastic flow via optical microscopy, and investigations of the phenomena in the vicinity of the crack, for which we used scanning and transmission electron microscopy. Numerical simulation was implemented not only to characterize the plastic flow more in detail, but primarily to investigate the probability of the occurrence of cracking based on the presence of stress. Last, but not least, microhardness in specific locations of the fitting were examined. The results reveal that the cracking occurring in the location with the highest probability of the occurrence of defects was most likely induced by differences in the chemical composition; the location the crack in which developed exhibited local changes not only in chemical composition—which manifested as the presence of brittle precipitates—but also in beta phase depletion. Moreover, as a result of the presence of oxidic precipitates and the hard and brittle alpha phase, the vicinity of the crack exhibited an increase in microhardness, which contributed to local brittleness.
Influence of Water Glass Introduction Methods on Selected Properties of Portland Cement
Authors: Szewczenko Wiktor , Kotsay Galyna
Published: June 2021

This article presents a study of the effect of water glass and its introduction on the hydration of Portland cement and its properties in plastic and solid states. The introduction of sodium water glass into the mixing water extends the setting time of Portland cement by 35%, while introduction into the cement paste reduces it by 24.4%; for potassium water glass, the respective values are 10.8% and 10.8%. The introduction of sodium water glass into the mixing water decreases its consistency by 17.6%; its introduction into the cement paste reduces its consistency by 97%. Based on microcalorimetric studies and using the modelling method, mechanisms of the processes occurring in the cement paste, for various methods of introducing water glass admixtures, and their influence on the properties of cement are proposed. The important implications of the obtained results are that, using various methods for introducing admixtures of water glass, it is possible to regulate the setting of cement slurries within significant limits that are important during their transportation.
Decoding Rocks: An Assessment of Geomaterial Microstructure Using X-ray Microtomography, Image Analysis and Multivariate Statistics
Authors: Strzelecki Piotr Jan , Świerczewska Anna , Kopczewska Katarzyna , Fheed Adam , Tarasiuk Jacek , Wroński Sebastian
Published: June 2021

An understanding of the microstructure of geomaterials such as rocks is fundamental in the evaluation of their functional properties, as well as the decryption of their geological history. We present a semi-automated statistical protocol for a complex 3D characterization of the microstructure of granular materials, including the clustering of grains and a description of their chemical composition, size, shape, and spatial properties with 44 unique parameters. The approach consists of an X-ray microtomographic image processing procedure, followed by measurements using image analysis and statistical multivariate analysis of its results utilizing freeware and widely available software. The statistical approach proposed was tested out on a sandstone sample with hidden and localized deformational microstructures. The grains were clustered into distinctive groups covering different compositional and geometrical features of the sample’s granular framework. The grains are pervasively and evenly distributed within the analysed sample. The spatial arrangement of grains in particular clusters is well organized and shows a directional trend referring to both microstructures. The methodological approach can be applied to any other rock type and enables the tracking of microstructural trends in grains arrangement.
Recent Progress in the Synthesis of MoS2 Thin Films for Sensing, Photovoltaic and Plasmonic Applications: A Review
Authors: Mouloua Driss , Kotbi Ahmed , Deokar Geetanjali , Kaja Khaled , El Marssi Mimoun , EL Khakani My Ali , Jouiad Mustapha
Published: June 2021

In the surge of recent successes of 2D materials following the rise of graphene, molybdenum disulfide (2D-MoS2) has been attracting growing attention from both fundamental and applications viewpoints, owing to the combination of its unique nanoscale properties. For instance, the bandgap of 2D-MoS2, which changes from direct (in the bulk form) to indirect for ultrathin films (few layers), offers new prospects for various applications in optoelectronics. In this review, we present the latest scientific advances in the field of synthesis and characterization of 2D-MoS2 films while highlighting some of their applications in energy harvesting, gas sensing, and plasmonic devices. A survey of the physical and chemical processing routes of 2D-MoS2 is presented first, followed by a detailed description and listing of the most relevant characterization techniques used to study the MoS2 nanomaterial as well as theoretical simulations of its interesting optical properties. Finally, the challenges related to the synthesis of high quality and fairly controllable MoS2 thin films are discussed along with their integration into novel functional devices.
Design of a Waterborne Polyurethane–Urea Ink for Direct Ink Writing 3D Printing
Authors: Vadillo Julen , Larraza Izaskun , Calvo-Correas Tamara , Gabilondo Nagore , Derail Christophe , Eceiza Arantxa
Published: June 2021

In this work, polycaprolactone–polyethylene glycol (PCL–PEG) based waterborne polyurethane–urea (WBPUU) inks have been developed for an extrusion-based 3D printing technology. The WBPUU, synthesized from an optimized ratio of hydrophobic polycaprolactone diol and hydrophilic polyethylene glycol (0.2:0.8) in the soft segment, is able to form a physical gel at low solid contents. WBPUU inks with different solid contents have been synthesized. The rheology of the prepared systems was studied and the WBPUUs were subsequently used in the printing of different pieces to demonstrate the relationship between their rheological properties and their printing viability, establishing an optimal window of compositions for the developed WBPUU based inks. The results showed that the increase in solid content results in more structured inks, presenting a higher storage modulus as well as lower tan δ values, allowing for the improvement of the ink’s shape fidelity. However, an increase in solid content also leads to an increase in the yield point and viscosity, leading to printability limitations. From among all printable systems, the WBPUU with a solid content of 32 wt% is proposed to be the more suitable ink for a successful printing performance, presenting both adequate printability and good shape fidelity, which leads to the realization of a recognizable and accurate 3D construct and an understanding of its relationship with rheological parameters.
Simplified Testing of the Bond Strength of Adhesives Used for Bonded Anchors
Authors: Barnat Jan , Prokeš Jan , Bajer Miroslav , Bezděk Ondřej , Vild Martin
Published: June 2021

The analysis presented in this paper is focused on problems of bond strength as an overall bond quality parameter of industrial adhesives for structural anchoring. In the first part, the problem of bond strength as the most important parameter influencing the final anchor resistance to tension load is described. Further in the text, a new methodology of simplified testing of the strength parameters of adhesives is described. Special test specimens made from steel are repeatedly used in this methodology. Additionally, results of these tests on some new recipes for adhesive are presented. Especially, epoxy resins with special fillers, such as carbon fibres, carbon nanotubes or graphene, were tested. The use of these adhesives in temperatures close to zero degrees Celsius was also tested.
Effect of Au-Coating on the Laser Spot Cutting on Spring Contact Probe (SCP) for Semi-Conductor Inspection
Authors: Seo Youngjin , Nam Jungsoo , Yun Huitaek , Jun Martin Byung Guk , Lee Dongkyoung
Published: June 2021

Spring contact probes (SCPs) are used to make contact with various test points on printed circuit boards (PCBs), wire harnesses, and connectors. Moreover, they can consist of the test interface between the PCBs and the semiconductor devices. For mass production of SCPs, ultra-small precision components have been manufactured by conventional cutting methods. However, these cutting methods adversely affect the performance of components due to tool wear and extreme shear stress at the contact point. To solve this problem, laser spot cutting is applied to Au-coated SCP specimens as an alternative technique. A 20 W nano-second pulsed Ytterbium fiber laser is used, and the experimental variables are different laser parameters including the pulse duration and repetition rate. After the spot cutting experiments, the heat-affected zone (HAZ) and material removal zone (MRZ) formed by different total irradiated energy (Etotal) was observed by using a scanning electron microscope (SEM). Then, the size of HAZ, top and bottom parts of MRZ, and roundness were measured. Furthermore, the change rate of HAZ and MRZ on Au-coated and non-coated specimens was analyzed with regard to different pulse durations. Based on these results, the effect of Au-coating on the SCP was evaluated through the comparison with the non-coated specimen. Consequently, in the Au-coated specimen, hole penetration was observed at a low pulse duration and low total energy due to the higher thermal conductivity of Au. From this study, the applicability of laser spot cutting to Au-coated SCP is investigated.
A Novel Surface Modification Strategy via Photopolymerized Poly-Sulfobetaine Methacrylate Coating to Prevent Bacterial Adhesion on Titanium Surfaces
Authors: Gülses Aydin , Lopar Antonia , Es-Souni Martha , Emmert Marie , Es-Souni Mona , Behrens Eleonore , Naujokat Hendrik , Liedtke Kim Rouven , Acil Yahya , Wiltfang Jörg , Flörke Christian
Published: June 2021

Recent investigations on the anti-adhesive properties of polysulfobetaine methacrylate (pSBMA) coatings had shown promising potential as antifouling surfaces and have given the impetus for the present paper, where a pSBMA coating is applied via photopolymerization on a macro-roughened, sandblasted, and acid-etched titanium implant surface in order to assess its antifouling properties. Current emphasis is placed on how the coating is efficient against the adhesion of Enterococcus faecalis by quantitative assessment of colony forming units and qualitative investigation of fluorescence imaging and scanning electron microscopy. pSBMA coatings via photopolymerization of titanium surfaces seems to be a promising antiadhesion strategy, which should bring substantial benefits once certain aspects such as biodegradation and osseointegration were addressed. Additionally, commercial SAL-titanium substrates may be coated with the super-hydrophilic coating, appearing resistant to physiological salt concentrations and most importantly lowering E. faecalis colonization significantly, compared to titanium substrates in the as-received state. It is very likely that pSBMA coatings may also prevent the adhesion of other germs.
Series in Le Roy Type Functions: A Set of Results in the Complex Plane—A Survey
Authors: Paneva-Konovska Jordanka
Published: June 2021

This study is based on a part of the results obtained in the author’s publications. An enumerable family of the Le Roy type functions is considered herein. The asymptotic formula for these special functions in the cases of ‘large’ values of indices, that has been previously obtained, is provided. Further, series defined by means of the Le Roy type functions are considered. These series are studied in the complex plane. Their domains of convergence are given and their behaviour is investigated ‘near’ the boundaries of the domains of convergence. The discussed asymptotic formula is used in the proofs of the convergence theorems for the considered series. A theorem of the Cauchy–Hadamard type is provided. Results of Abel, Tauber and Littlewood type, which are analogues to the corresponding theorems for the classical power series, are also proved. At last, various interesting particular cases of the discussed special functions are considered.
The Scalar Curvature of a Riemannian Almost Paracomplex Manifold and Its Conformal Transformations
Authors: Rovenski Vladimir , Mikeš Josef , Stepanov Sergey
Published: June 2021

A Riemannian almost paracomplex manifold is a 2n-dimensional Riemannian manifold (M,g), whose structural group O(2n,R) is reduced to the form O(n,R)×O(n,R). We define the scalar curvature π of this manifold and consider relationships between π and the scalar curvature s of the metric g and its conformal transformations.
Reducing the Computational Time for the Kemeny Method by Exploiting Condorcet Properties
Authors: Rico Noelia , Vela Camino R. , Pérez-Fernández Raúl , Díaz Irene
Published: June 2021

Preference aggregation and in particular ranking aggregation are mainly studied by the field of social choice theory but extensively applied in a variety of contexts. Among the most prominent methods for ranking aggregation, the Kemeny method has been proved to be the only one that satisfies some desirable properties such as neutrality, consistency and the Condorcet condition at the same time. Unfortunately, the problem of finding a Kemeny ranking is NP-hard, which prevents practitioners from using it in real-life problems. The state of the art of exact algorithms for the computation of the Kemeny ranking experienced a major boost last year with the presentation of an algorithm that provides searching time guarantee up to 13 alternatives. In this work, we propose an enhanced version of this algorithm based on pruning the search space when some Condorcet properties hold. This enhanced version greatly improves the performance in terms of runtime consumption.
Acceptability of Healthcare Professionals to Get Vaccinated against COVID-19 Two Weeks before Initiation of National Vaccination
Authors: Pataka Athanasia , Kotoulas Seraphim , Stefanidou Emilia , Grigoriou Ioanna , Tzinas Asterios , Tsiouprou Ioanna , Zarogoulidis Paul , Courcoutsakis Nikolaos , Argyropoulou Paraskevi
Published: June 2021

Background and Objectives The greatest challenge vaccines face is that of acceptance from the general population. Healthcare professionals’ (HCPs) recommendations have significant influence on general public vaccination behavior. The aim of this study was to assess the willingness of HCPs to get vaccinated against COVID-19, two weeks before initiation of vaccinations. Materials and Methods: We conducted an anonymous online survey from 11–15 December 2020 among HCPs by emails delivered from the local medical and nursing stuff associations. Results: The 71.1% of 656 HCPs intended to accept vaccination, 5.9% did not and 23% were still undecided. The acceptance rate was higher in physicians (76.5%) and significantly lower in nurses (48.3%). Most of the responders who intended to accept vaccination were males (p = 0.01), physicians (p = 0.001), older (p = 0.02), married (p = 0.054) with children (p = 0.001), and had treated patients with COVID-19 (p < 0.001). In the multivariate logistic regression, the predictors of HCPs willingness to get vaccinated were parenthood (OR = 4.19, p = 0.003), being a physician (OR = 2.79, p = 0.04), and treating confirmed/suspected COVID-19 patients (OR = 2.87, p = 0.036). Conclusions: Low vaccination acceptance rate was found especially in nurses, and as this may have a negative impact in the vaccination compliance of the general public, interventional educational programs to enhance vaccination are crucial.
Adolescence, Adulthood and Self-Perceived Halitosis: A Role of Psychological Factors
Authors: Mento Carmela , Lombardo Clara , Milazzo Mariacristina , Whithorn Nicholas Ian , Boronat-Catalá Montserrat , Almiñana-Pastor Pedro J. , Fernàndez Cristina Sala , Bruno Antonio , Muscatello Maria Rosaria Anna , Zoccali Rocco Antonio
Published: June 2021

(1) Background: Halitosis is a frequent condition that affects a large part of the population. It is considered a “social stigma”, as it can determine a number of psychological and relationship consequences that affect people’s lives. The purpose of this review is to examine the role of psychological factors in the condition of self-perceived halitosis in adolescent subjects and adulthood. (2) Type of studies reviewed: We conducted, by the PRISMA (Preferred Reporting Items for Systematic Review and Meta-Analyses) guidelines, systematic research of the literature on PubMed and Scholar. The key terms used were halitosis, halitosis self-perception, psychological factors, breath odor and two terms related to socio-relational consequences (“Halitosis and Social Relationship” OR “Social Issue of Halitosis”). Initial research identified 3008 articles. As a result of the inclusion and exclusion criteria, the number of publications was reduced to 38. (3) Results: According to the literature examined, halitosis is a condition that is rarely self-perceived. In general, women have a greater ability to recognize it than men. Several factors can affect the perception of the dental condition, such as socioeconomic status, emotional state and body image. (4) Conclusion and practical implication: Self-perceived halitosis could have a significant impact on the patient’s quality of life. Among the most frequent consequences are found anxiety, reduced levels of self-esteem, misinterpretation of other people’s attitudes and embarrassment and relational discomfort that often result in social isolation.
Meibomian Gland Dysfunction: Intense Pulsed Light Therapy in Combination with Low-Level Light Therapy as Rescue Treatment
Authors: Solomos Leonidas , Bouthour Walid , Malclès Ariane , Thumann Gabriele , Massa Horace
Published: June 2021

Background and Objectives: Evaporative dry eye disease is frequently associated with meibomian gland dysfunction. Patients are often unhappy because of daily drops, care burden, and suboptimal conventional treatments. In this study, we assessed the efficacy of a novel device, the Eye-light®, a combination of intense pulsed light therapy and low-level light therapy, as a novel treatment for meibomian gland dysfunction and dry eye disease. Materials and Methods: This was a retrospective, single-center study carried out over a 6-week period, in which 22 eyes from 11 patients were included. Each patient received four combined light therapy treatment sessions, once weekly over 4 weeks. Patients underwent a clinical examination and filled out a standardized questionnaire to evaluate symptoms one week prior to treatment, and one week after the fourth session. Results: Combined light therapy improved several ocular surface outcome measures in our patients. This study demonstrates that this adjunctive treatment significantly improves the ocular surface and quality of life of patients with dry eye disease and meibomian gland dysfunction. Conclusions: Combined light therapy may be included in meibomian gland dysfunction treatment protocols as an adjunctive rescue treatment.
Numerical Study of Coupled Electrical-Thermal-Mechanical-Wear Behavior in Electrical Contacts
Authors: Shen Fei , Ke Liao-Liang
Published: June 2021

Electrical contacts involve complicated electrical, thermal, and mechanical phenomena. Fretting wear as a surface damage mechanism significantly weakens the performance of electrical contact components. In this study, a numerical approach is developed to investigate the electrical-thermal-mechanical-wear coupling behavior of electrical contacts. An electrical contact conductance law is used with the current conservation model to evaluate the electrical behavior. A transient heat transfer model, including the Joule heating behavior and a thermal contact conductance law, is employed to calculate the temperature field. Both contact conductance laws are related to the contact pressure distribution obtained by the contact stress analysis. Based on the predicted contact stress and relative slip on contact surfaces, the energy wear model is used to study the evolution of fretting wear depth and contact surface geometry. The material properties in these models are temperature-dependent. The proposed numerical approach is implemented in a finite element modeling of electrical contacts, which is validated by comparing the predicted and experimental results of the wear scar profile. The effects of the fretting wear on the electric potential, current density, contact resistance, temperature, and contact pressure are numerically studied.
Influence of Tool Wear on Form Deviations in Dry Machining of UNS A97075 Alloy
Authors: Trujillo Vilches Francisco Javier , Martín Béjar Sergio , Bermudo Gamboa Carolina , Herrera Fernández Manuel , Sevilla Hurtado Lorenzo
Published: June 2021

Geometrical tolerances play a very important role in the functionality and assembly of parts made of light alloys for aeronautical applications. These parts are frequently machined in dry conditions. Under these conditions, the tool wear becomes one of the most important variables that influence geometrical tolerances. In this work, the influence of tool wear on roundness, straightness and cylindricity of dry-turned UNS A97075 alloy has been analyzed. The tool wear and form deviations evolution as a function of the cutting parameters and the cutting time has been assessed. In addition, the predominant tool wear mechanisms have been checked. The experimental results revealed that the indirect adhesion wear (BUL and BUE) was the main tool-wear mechanism, with the feed being the most influential cutting parameter. The combination of high feed and low cutting speed values resulted in the highest tool wear. The analyzed form deviations showed a general trend to increase with both cutting parameters. The tool wear and the form deviations tend to increase with the cutting time only within the intermediate range of feed tested. As the main novelty, a relationship between the cutting parameters, the cutting time (and, indirectly, the tool wear) and the analyzed form deviations has been found.
Dried Blood Spot (DBS) Methodology Study for Biomarker Discovery in Lysosomal Storage Disease (LSD)
Authors: Rus Corina-Marcela , Di Bucchianico Sebastiano , Cozma Claudia , Zimmermann Ralf , Bauer Peter
Published: June 2021

Lysosomal storage diseases (LSDs) are a heterogeneous group of inherited metabolic diseases caused by mutations in genes encoding for proteins involved in the lysosomal degradation of macromolecules. They occur in approximately 1 in 5000 live births and pose a lifelong risk. Therefore, to achieve the maximum benefit from LSDs therapies, a fast and early diagnosis of the disease is required. In this framework, biomarker discovery is a significant factor in disease diagnosis and in predicting its outcomes. On the other hand, the dried blood spot (DBS) based metabolomics platform can open up new pathways for studying non-directional hypothesis approaches to biomarker discovery. This study aims to increase the efficiency of the developed methods for biomarker development in the context of rare diseases, with an improved impact on the reliability of the detected compounds. Thereby, we conducted two independent experiments and integrated them into the screening of the human blood metabolome: (1) comparison of EDTA blood and filter cards in terms of their suitability for metabolomics studies; (2) optimization of the extraction method: a side-by-side comparison of a series of buffers to the best utility to the disease of interest. The findings were compared to previous studies across parameters such as metabolite coverage, sample type suitability, and stability. The results indicate that measurements of metabolites are susceptible to differences in pre-analytical conditions and extraction solvents. This proposed approach can increase the positive rate of the future development of biomarkers. Altogether, the procedure can be easily adapted and applied to other studies, where the limited number of samples is a common barrier.
Pre-Degassed Microfluidic Chamber-Based Digital PCR Device for Meat Authentication Applications
Authors: Hu Hezhi , Cheng Jingmeng , Wei Chunyang , Li Shanshan , Yu Chengzhuang , Meng Xiaoshuai , Li Junwei
Published: June 2021

Droplet digital polymerase chain reaction (ddPCR) suffers from the need for specific equipment and skilled personnel; thus, we here present a chamber-based digital PCR microfluidic device that is compatible with fluorescence image read-out systems and removes bubbles by a pre-degassed microfluidic device that consists of a pilot channel and micro chamber arrays. Digitalized PCR reagents are introduced into micro chambers, and thermocycles are taken to perform a DNA amplification process. Then, fluorescence images of a micro chamber array are read out and analyzed to obtain the total number of positive chambers. Thereby, the copy numbers of target DNA are calculated for quantitative detections. As a validation, this device is evaluated by the application of meat authentication. We performed dPCR tests using DNA templates extracted from a pure mutton DNA template with different dilutions. Then, the dPCR chip was used to identify the meat authentication using mutton–chicken mixtures with different mass ratios, showing its performance in real biotechnical applications.
Methods of Respiratory Virus Detection: Advances towards Point-of-Care for Early Intervention
Authors: Lu Siming , Lin Sha , Zhang Hongrui , Liang Liguo , Shen Shien
Published: June 2021

Respiratory viral infections threaten human life and inflict an enormous healthcare burden worldwide. Frequent monitoring of viral antibodies and viral load can effectively help to control the spread of the virus and make timely interventions. However, current methods for detecting viral load require dedicated personnel and are time-consuming. Additionally, COVID-19 detection is generally relied on an automated PCR analyzer, which is highly instrument-dependent and expensive. As such, emerging technologies in the development of respiratory viral load assays for point-of-care (POC) testing are urgently needed for viral screening. Recent advances in loop-mediated isothermal amplification (LAMP), biosensors, nanotechnology-based paper strips and microfluidics offer new strategies to develop a rapid, low-cost, and user-friendly respiratory viral monitoring platform. In this review, we summarized the traditional methods in respiratory virus detection and present the state-of-art technologies in the monitoring of respiratory virus at POC.
Beneficial Insects Deliver Plant Growth-Promoting Bacterial Endophytes between Tomato Plants
Authors: Galambos Nikoletta , Compant Stéphane , Wäckers Felix , Sessitsch Angela , Anfora Gianfranco , Mazzoni Valerio , Pertot Ilaria , Perazzolli Michele
Published: June 2021

Beneficial insects and mites, including generalist predators of the family Miridae, are widely used in biocontrol programs against many crop pests, such as whiteflies, aphids, lepidopterans and mites. Mirid predators frequently complement their carnivore diet by feeding plant sap with their piercing–sucking mouthparts. This implies that mirids may act as vectors of phytopathogenic and beneficial microorganisms, such as plant growth-promoting bacterial endophytes. This work aimed at understanding the role of two beneficial mirids (Macrolophus pygmaeus and Nesidiocoris tenuis) in the acquisition and transmission of two plant growth-promoting bacteria, Paraburkholderia phytofirmans strain PsJN (PsJN) and Enterobacter sp. strain 32A (32A). Both bacterial strains were detected on the epicuticle and internal body of both mirids at the end of the mirid-mediated transmission. Moreover, both mirids were able to transmit PsJN and 32A between tomato plants and these bacterial strains could be re-isolated from tomato shoots after mirid-mediated transmission. In particular, PsJN and 32A endophytically colonised tomato plants and moved from the shoots to roots after mirid-mediated transmission. In conclusion, this study provided novel evidence for the acquisition and transmission of plant growth-promoting bacterial endophytes by beneficial mirids.
Nasal Methicillin-Resistant Staphylococcus aureus Colonization in Patients with Type 1 Diabetes in Taiwan
Authors: Kang Chun-Ya , Kang Eugene Yu-Chuan , Lai Chi-Chun , Lo Wei-Che , Chen Kun-Jen , Wu Wei-Chi , Liu Laura , Hwang Yih-Shiou , Lo Fu-Sung , Huang Yhu-Chering
Published: June 2021

Nasal methicillin-resistant Staphylococcus aureus (MRSA) colonies are an essential reservoir of infection, especially for patients with diabetes. However, data on MRSA colonization in patients with type 1 diabetes are limited. We investigated the epidemiology of MRSA colonization in patients with type 1 diabetes. This prospective cross-sectional study was conducted in a medical center (Chang Gung Memorial Hospital) in Taiwan from 1 July to 31 December 2020. Nasal sampling and MRSA detection were performed. The molecular characteristics of MRSA isolates were tested, and factors associated with MRSA colonization were analyzed. We included 245 patients with type 1 diabetes; nasal MRSA colonization was identified in 13 (5.3%) patients. All isolates belonged to community-associated MRSA genetic strains; the most frequent strain was clonal complex 45 (53.8%), followed by ST59 (30.8%) (a local community strain). MRSA colonization was positively associated with age ≤ 10 years, body mass index < 18 kg/m2, and diabetes duration < 10 years; moreover, it was negatively associated with serum low-density lipoprotein cholesterol ≥ 100 mg/dL. No independent factor was reported. The nasal MRSA colonization rate in type 1 diabetes is approximately 5% in Taiwan. Most of these colonizing strains are community strains, namely clonal complex 45 and ST59.
Lead Isotopes to Identify Underwater Ceramic Contamination: The Example of the Kyrenia Shipwreck (Cyprus)
Authors: Renson Virginie , Glascock Michael D.
Published: June 2021

We present the lead isotopic composition of ceramic fragments from the Kyrenia shipwreck (Cyprus), selected from three chemical groups related to the Rhodes and Alimos regions (Greece). Fragments of the lead sheathing covering the ship’s hull and biogenic material formed on some of the ceramic sherds, were analyzed along with sherds from the Rhodes and Alimos source areas for comparison. The objective of this paper was to evaluate the impact of the seawater environment on the isotopic signature of the ceramics, and on our ability to use lead isotopes to source ceramics recovered from seawater. The results showed that the lead isotopic composition of the shipwreck ceramics was modified by its prolonged proximity to the lead hull sheathing. The lead signature of filtering marine organisms encrusted on the ceramics provided support for this hypothesis.
Primary Minerals and Age of The Hydrothermal Quartz Veins Containing U-Mo-(Pb, Bi, Te) Mineralization in the Majerská Valley near Čučma (Gemeric Unit, Spišsko-Gemerské Rudohorie Mts., Slovak Republic)
Authors: Ferenc Štefan , Števko Martin , Mikuš Tomáš , Milovská Stanislava , Kopáčik Richard , Hoppanová Eva
Published: June 2021

An occurrence of vein U-Mo mineralization is located in the Majerská valley near Čučma, about 7 km to the NNE of the district town of Rožňava (Eastern Slovakia). Mineralization is hosted in the acidic metapyroclastics of the Silurian Bystrý Potok Fm. (Gemeric Unit), and originated in the following stages: (I.) quartz I, fluorapatite I; (II.) quartz II, fluorapatite II, zircon, rutile chlorite, tourmaline; (III.) uraninite, molybdenite, U-Ti oxides; (IV.) pyrite I, ullmannite, gersdorffite, cobaltite; (Va.) galena, bismuth, tetradymite, joséite A and B, Bi3(TeS)2 mineral phase, (BiPb)(TeS) mineral phase, ikunolite; (Vb.) minerals of the kobellite–tintinaite series, cosalite; (VI.) pyrite II; (VII.) titanite, chlorite; and (VIII.) supergene mineral phases. The chemical in-situ electron-microprobe U-Pb dating of uraninite from a studied vein yielded an average age of around 265 Ma, corresponding to the Guadalupian Epoch of Permian; the obtained data corresponds with the age of Gemeric S-type granites. The age correlation of uraninite with the Gemeric S-type granites and the spatial connection of the studied mineralization with the Čučma granite allows us to assume that it is a Hercynian, granite-related (perigranitic) mineralization.