bluuebunny/crossref_metadata_2025 huggingface dataset:
Modeling and identification of the Quanser Aero using a detailed description of friction and centripetal forces
Authors: Dyvik Mathias , Fjereide Didrik Efjestad , Rotondo Damiano
Published: October 2023

This paper deals with the modeling and identification of the Quanser Aero. The Quanser Aero is an aerospace laboratory setup designed for teaching aerospace concepts. Two propellers generate thrust and allow the user to control its dynamic response. The ability to lock axes individually makes it capable of abstracting a variety of aerospace systems, such as half-quadrotor, 1-Degree of freedom (DOF), vertical take-off and landing (VTOL), and 2-DOF helicopter. This paper focuses on the latter of these modes. In this configuration, the Quanser Aero can produce different pitch and yaw angles based on the angular velocity of the propellers, which produces an interesting identification and control problem, due to the presence of nonlinearities and significant cross-couplings between different variables. In this paper, a nonlinear model derived from Newton’s law and Euler’s rotational dynamics is obtained, and the unknown model parameters are identified through an experimental approach, with the model validated through real-time testing. In particular, it is shown that by means of a more detailed description of the friction, which includes the Karnopp’s model that keeps the sum of the magnitude of all forces equal to zero until the applied forces are strong enough to overcome the friction force, and of the centripetal forces acting on the Aero, significant improvements are obtained when compared to state-of-the-art models. These improvements may hold the potential to enhance the performance of advanced nonlinear model-based control algorithms for this device.
Enhancing Indoor Environmental Simulations: A Comprehensive Review of CFD Methods
Authors: Marashian Shahrzad , Vadiee Amir , Abouali Omid , Sadrizadeh Sasan
Published: October 2023

Computational Fluid Dynamics (CFD) simulations are extensively used to model indoor environments, including airflow patterns, temperature distribution, and contaminant dispersion. These simulations provide valuable insights for improving indoor air quality, enhancing thermal comfort, optimizing energy efficiency, and informing design decisions. The recent global pandemic has emphasized the importance of understanding airflow patterns and particle dispersion in indoor spaces, highlighting the potential of CFD simulations to guide strategies for improving indoor air quality and public health. Consequently, there has been a significant increase in research focused on studying the transport and dispersion of pollutants in indoor environments using CFD techniques. These simulations are vital in advancing engineers' understanding of indoor environments; however, achieving accurate results requires careful method selection and proper implementation of each step. This paper aims to review the state-of-the-art CFD simulations of indoor environments, specifically focusing on strategies employed for three main simulation components: geometry and grid generation, ventilation strategies, and turbulence model selection. Researchers can select suitable techniques for their specific applications by comparing different indoor airflow simulation strategies.
Sustainable application of rice-waste for fuels and valuable chemicals-a mini review
Authors: Zhang Wenwen , Xu Xiaoyu , Yuan Yongjun , Wang Zichun
Published: October 2023

The global annual production of rice is over 750 million tons, and generates a huge amount of biomass waste, such as straw, husk, and bran, making rice waste an ideal feedstock for biomass conversion industries. This review focuses on the current progress in the transformation of rice waste into valuable products, including biochar, (liquid and gaseous) biofuels, valuable chemicals (sugars, furan derivatives, organic acids, and aromatic hydrocarbons), and carbon/silicon-based catalysts and catalyst supports. The challenges and future prospectives are highlighted to guide future studies in rice waste valorization for sustainable production of fuels and chemicals.
Effect of composite conjugated materials on tissue healing during exercise rehabilitation training
Authors: Li Jie , Zhang Jing
Published: October 2023

The application of traditional materials to tissue healing in sports rehabilitation training has problems such as poor effect, high rejection reaction, and slow healing speed. It also brings many challenges to the development of sports rehabilitation training. This article aims to explore the impact of composite conjugated materials on tissue healing to promote rapid and efficient tissue healing and improve the effect of sports rehabilitation training. Through research and analysis, this article found that composite conjugated materials have unique biocompatibility and can promote cell growth and differentiation. In skin tissue healing, composite conjugated materials can control the release rate and duration of drugs to promote skin healing. During the fracture healing process, conjugated materials can provide growth factors and extracellular matrix components, stimulate bone cell proliferation and differentiation, and promote fracture healing. In terms of soft tissue injuries, composite conjugated materials serve as supporting structures or matrices, providing a favorable environment for the regeneration of damaged tissue. In the regulation of inflammatory responses, composite conjugated materials reduce inflammatory responses and accelerate the healing process by modulating immune responses. The results of this study show that 1 week after the experiment, the skin healing rates of the control group and the experimental group were 42.55% and 58.17% respectively; 5 weeks after the experiment, the skin healing rates of the control group and the experimental group were 51.28% and 73.24% respectively. After 1, 2, 3, 4, and 5 weeks of experiment, it was found that the average tissue repair rates of the control group were 44.03%, 54.18%, 58.40%, 67.08%, and 72.09% respectively, and the average tissue repair rates of the experimental group were 52.18%, 61.91%, 63.40%, 74.61%, and 85.05% respectively. This study highlights the huge potential of composite conjugated materials in promoting tissue healing and tissue repair, and is of great significance for promoting technological progress in the field of sports rehabilitation and improving rehabilitation effects.
Significance of RNA N6-methyladenosine regulators in the diagnosis and subtype classification of coronary heart disease using the Gene Expression Omnibus database
Authors: Jiang Yu , Pan Yaqiang , Long Tao , Qi Junqing , Liu Jianchao , Zhang Mengya
Published: October 2023

Background

Many investigations have revealed that alterations in m6A modification levels may be linked to coronary heart disease (CHD). However, the specific link between m6A alteration and CHD warrants further investigation.

Methods

Gene expression profiles from the Gene Expression Omnibus (GEO) databases. We began by constructing a Random Forest model followed by a Nomogram model, both aimed at enhancing our predictive capabilities on specific m6A markers. We then shifted our focus to identify distinct molecular subtypes based on the key m6A regulators and to discern differentially expressed genes between the unique m6A clusters. Following this molecular exploration, we embarked on an in-depth analysis of the biological characteristics associated with each m6A cluster, revealing profound differences between them. Finally, we delved into the identification and correlation analysis of immune cell infiltration across these clusters, emphasizing the potential interplay between m6A modification and the immune system.

Results

In this research, 37 important m6Aregulators were identified by comparing non-CHD and CHD patients from the GSE20680, GSE20681, and GSE71226 datasets. To predict the risk of CHD, seven candidate m6A regulators (CBLL1, HNRNPC, YTHDC2, YTHDF1, YTHDF2, YTHDF3, ZC3H13) were screened using the logistic regression model. Based on the seven possible m6A regulators, a nomogram model was constructed. An examination of decision curves revealed that CHD patients could benefit from the nomogram model. On the basis of the selected relevant m6A regulators, patients with CHD were separated into two m6A clusters (cluster1 and cluster2) using the consensus clustering approach. The Single Sample Gene Set Enrichment Analysis (ssGSEA) and CIBERSORT methods were used to estimate the immunological characteristics of two separate m6A Gene Clusters; the results indicated a close association between seven candidate genes and immune cell composition. The drug sensitivity of seven candidate regulators was predicted, and these seven regulators appeared in numerous diseases as pharmacological targets while displaying strong drug sensitivity.

Conclusion

m6A regulators play crucial roles in the development of CHD. Our research of m6A clusters may facilitate the development of novel molecular therapies and inform future immunotherapeutic methods for CHD.
Prevalence and temporal trends of prostate diseases among inpatients with cardiovascular disease: a nationwide real-world database survey in Japan
Authors: Kaneta Kohei , Tanaka Atsushi , Nakai Michikazu , Sumita Yoko , Kaneko Hidehiro , Noguchi Mitsuru , Node Koichi
Published: October 2023

Introduction

Benign prostate hyperplasia (BPH) and prostate cancer (PCa) are major prostate diseases that potentially share cardiometabolic risk factors and an elevated risk for cardiovascular disease (CVD). However, the prevalence of prostate diseases among patients with established CVD remains unclear.

Materials and methods

This nationwide retrospective study assessed the prevalence and temporal trend of prostate diseases (i.e., BPH or PCa) among patients hospitalized for CVDs in Japan. We used a claims database (the Japanese Registry of All Cardiac and Vascular Diseases–Diagnosis Procedure Combination), which included data on 6,078,487 male patients recorded from 1,058 hospitals between April 2012 and March 2020. We conducted the Cochran–Armitage trend test and calculated the adjusted odds ratio (aOR) with 95% confidence intervals (CIs).

Results

The prevalence of prostate diseases over the entire study period was 5.7% (BPH, 4.4%; PCa, 1.6%). When dividing the overall cohort into age categories (<65, 65–74, and ≥75 years old), the prevalence was 1.1%, 4.7%, and 9.9%, respectively (P for trend <0.05). In addition, the annual prevalence showed a modest increasing trend over time. Patients admitted for heart failure (HF) were significantly associated with a higher incidence of coexisting prostate diseases than those admitted for non-HF causes [aOR 1.02 (95% CI, 1.01–1.03)] or acute coronary syndrome [aOR 1.19 (95% CI, 1.17–1.22)].

Conclusions

The nationwide real-world database revealed that the prevalence of prostate diseases is increasing among patients hospitalized for CVD, particularly HF. Attention to detailed causality and continued surveillance are needed to further clarify the clinical characteristics of prostate diseases among patients with CVD.
On the external forcing of global eruptive activity in the past 300 years
Authors: Le Mouël J-L. , Gibert D. , Courtillot V. , Dumont S. , de Bremond d'Ars J. , Petrosino S. , Zuddas P. , Lopes F. , Boulé J-B. , Neves M. C. , Custódio S. , Silveira G. , Kossobokov V. , Coen L. , Gèze M.
Published: October 2023

The decryption of the temporal sequence of volcanic eruptions is a key step in better anticipating future events. Volcanic activity results from a complex interaction between internal and external processes, with time scales spanning multiple orders of magnitude. We review periodicities that have been detected or correlated with volcanic eruptions/phenomena and interpreted as resulting from external forces. Taking a global perspective and longer time scales than a few years, we approach this interaction by analyzing three time-series using singular spectral analysis: the global number of volcanic eruptions (NVE) between 1700 and 2022, the number of sunspots (ISSN), a proxy for solar activity, and polar motion (PM) and length of day (lod), two proxies for gravitational force. Several pseudo-periodicities are common to NVE and ISSN, in addition to the 11-year Schwabe cycle that has been reported in previous work, but NVE shares even more periodicities with PM. These quasi-periodic components range from −5 to −130 years. We interpret our analytical results in light of the Laplace’s paradigm and propose that, similarly to the movement of Earth’s rotation axis, global eruptive activity is modulated by commensurable orbital moments of the Jovian planets together with Pluto, whose influence is also detected in solar activity.
Signaling pathways and targeted therapeutic strategies for polycystic ovary syndrome
Authors: Wang Kexin , Li Yanhua
Published: October 2023

Polycystic ovary syndrome (PCOS) is the most common endocrine disorder among women of reproductive age. Although promising strides have been made in the field of PCOS over the past decades, the distinct etiologies of this syndrome are not fully elucidated. Prenatal factors, genetic variation, epigenetic mechanisms, unhealthy lifestyles, and environmental toxins all contribute to the development of this intricate and highly heterogeneous metabolic, endocrine, reproductive, and psychological disorder. Moreover, interactions between androgen excess, insulin resistance, disruption to the hypothalamic–pituitary–ovary (HPO) axis, and obesity only make for a more complex picture. In this review, we investigate and summarize the related molecular mechanisms underlying PCOS pathogenesis from the perspective of the level of signaling pathways, including PI3K/Akt, TGF-β/Smads, Wnt/β-catenin, and Hippo/YAP. Additionally, this review provides an overview of prospective therapies, such as exosome therapy, gene therapy, and drugs based on traditional Chinese medicine (TCM) and natural compounds. By targeting these aberrant pathways, these interventions primarily alleviate inflammation, insulin resistance, androgen excess, and ovarian fibrosis, which are typical symptoms of PCOS. Overall, we hope that this paper will pave the way for better understanding and management of PCOS in the future.
Molecular interplay between ecdysone receptor and retinoid X receptor in regulating the molting of the Chinese mitten crab, Eriocheir sinensis
Authors: Chen Xiaowen , Hou Xin , Yang Hao , Liu Hao , Wang Jun , Wang Chenghui
Published: October 2023

Purpose

Molting is a pivotal biological process regulated by the ecdysteroid signaling pathway that requires molecular coordination of two transcription factors, Ecdysone receptor (EcR) and ultraspiracle (USP) in arthropods. However, the molecular interplay of EcR and Retinoid X receptor (RXR), the crustacean homolog of USP in the ecdysteroid signaling pathway, is not well understood.

Methods

In this study, we conducted temporal and spatial expression, co-immunoprecipitation (CO-IP), and luciferase reporter assay experiments to investigate the molecular function and interplay of EcR and RXR during the molting process of the Chinese mitten crab, Eriocheir sinensis.

Results

The results showed that the expression level of RXR was more stable and significantly higher than EcR during the entire molting process. However, the expression level of EcR fluctuated dynamically and increased sharply at the premolt stage. The CO-IP and luciferase reporter assay results confirmed the molecular interplay of EcR and RXR. The heterodimer complex formed by the two transcription factors significantly induced the transcription of E75, an essential gene in the ecdysteroid signaling pathway.

Conclusions

Our study unveiled the diverse molecular function and molecular interplay of EcR and RXR; RXR is possibly a “constitutive-type” gene, and EcR is possibly a vital speed-limiting gene while both EcR and RXR are required to initiate the ecdysteroid signaling cascade, which may be indispensable for molting regulation in E. sinensis. The results provide a theoretical basis for the endocrine control of molting in E. sinensis and novel insights into the molecular mechanism of molting mediated by the ecdysteroid signaling pathway in crustaceans.
Mating with dead conspecifics in an invasive ladybird is affected by male sexual fasting and time since the female’s death
Authors: Řeřicha Michal , Montoya-Molina Santiago , Harabiš Filip , Knapp Michal
Published: October 2023

High promiscuity and low mating partner choosiness in insects can sometimes result in a deviated mating behaviour such as mating with already dead individuals. In this study we investigated the occurrence of necrophilic behaviour in males of the invasive ladybird Harmonia axyridis using two laboratory experiments. For both no-choice and choice experiments, the probability of mating and mating duration were significantly affected by female status (alive, 1-day, 7-day, or 14-day old carcass) and by male mating status (unmated or mated) which was investigated in the no-choice experiment. The presence of chemical cues produced by an alive female did not affect the probability of a male mating with a dead female. In the no-choice experiment, 14-day old female carcasses were significantly less attractive than alive females, and unmated males tended to mate with females of all statuses with significantly higher probability than mated males. In the choice experiment, males showed a significant preference to mate with alive females when compared to 7-day old carcasses but did not distinguish between alive females and 1-day old carcasses. Mating latency (time to the starting of mating) tended to be longer for 14-day old carcasses in the no-choice experiment and was not affected by female status in the choice experiment. Mating duration was negatively affected by age of female carcasses in the no-choice experiment and only 7-day old carcasses were mated for significantly shorter time than alive females in the choice experiment. Mating behaviour was highly consistent for the no-choice and choice experiments. Despite decreasing probability of mating with 7-day and 14-day old carcasses, we observed a surprisingly high frequency of mating with dead conspecifics in the invasive ladybird Harmonia axyridis, which could have serious consequences for transmission of pathogens and affect male fitness even under natural conditions.
IGFBP2 from a novel copper metabolism-associated biomarker promoted glioma progression and response to immunotherapy
Authors: Luo Qisheng , Zhuang Junhong , Zheng Dandan , Miao Changfeng , Luo Hongcheng , Peng Jun , Zheng Chuanhua , Qin Chengjian , Lan Chuanliu , Chen Meiqin , Xia Ying , Huang Deyou , Chen Zigui
Published: October 2023

Introduction

Copper metabolism encompasses all cellular metabolic processes involving copper ions and plays a significant role in the pathogenesis of diseases, including cancer. Furthermore, copper is intricately involved in various processes related to nucleotide metabolism. However, a comprehensive analysis of copper metabolism in gliomas remains lacking despite its importance.

Methods

To address this gap, glioma patients were stratified based on the expression levels of copper metabolism-related genes. By utilizing machine learning techniques, a novel copper metabolism-associated biomarker was developed. The potential of this biomarker in prognosis, mutation analysis, and predicting immunotherapy response efficiency in gliomas was systematically investigated.

Results

Notably, IGFBP2, identified as a glioma tumor promoter, was found to promote disease progression and influence immunotherapy response. Additionally, glioma-derived IGFBP2 was observed to enhance microglial migration. High IGFBP2 expression in GBM cells facilitated macrophage interactions through the EGFR, CD63, ITGB1, and CD44 signaling pathways. Discussion: Overall, the copper metabolism-associated biomarker shows promising potential to enhance the clinical management of gliomas, offering valuable insights into disease prognosis and treatment strategies.
A review of post-whaling abundance, trends, changes in distribution and migration patterns, and supplementary feeding of Southern Hemisphere humpback whales
Authors: Seyboth Elisa , Meynecke Jan-Olaf , de Bie Jasper , Roychoudhury Alakendra , Findlay Ken
Published: October 2023

Southern Hemisphere humpback whales (Megaptera novaeangliae) were heavily targeted during modern commercial whaling operations, with some 216,000 individuals killed between 1903 and 1973. That impacted the abundance of all the seven breeding stocks of the species. Most of these stocks have been recovering from whaling pressure although the understanding of the current growth rates of some stocks, and how the rates compare across stocks are lacking. Updated information is fundamental for understanding the species’ current status, and to support the review of management plans promoting its protection and recovery, especially considering current changes in ocean environments due to climate change. This work offers a comprehensive overview of the current knowledge on Southern Hemisphere humpback whales breeding stocks’ status. The aim is to provide information on their post-whaling growth trends and changes in distribution and migration patterns. Within that, records of supplementary feeding records (i.e. feeding beyond their formally described feeding grounds) are described. We have also identified knowledge gaps and note that the establishment of research collaborations, as well as standard methodologies for data collection can be important steps for the acquisition of better comparable data sets for the analysis of the current status of humpback whales and to fill such gaps. The compiled information provided can be used as part of an In-Depth Assessment of the species by the International Whaling Commission.
Pathological and virological insights from an outbreak of European brown hare syndrome in the Italian hare (Lepus corsicanus)
Authors: Domanico Mariagiovanna , Cavadini Patrizia , Nardini Roberto , Cecca Daniele , Mastrandrea Giovanni , Eleni Claudia , Galietta Valentina , Attili Lorenzo , Pizzarelli Antonella , Onorati Roberta , Amoruso Cristina , Stilli Donatella , Pacchiarotti Giulia , Merzoni Francesca , Caprioli Andrea , Ricci Ida , Battisti Antonio , Lavazza Antonio , Scicluna Maria Teresa
Published: October 2023

European brown hare syndrome (EBHS) is a highly contagious and fatal viral disease, mainly affecting European brown hares (Lepus europaeus). The etiological agent, EBHS virus (EBHSV), belongs to theLagovirusgenus within theCaliciviridaefamily. The Italian hare (Lepus corsicanus) is endemic to Central-Southern Italy and Sicily and is classified as a vulnerable species.L. corsicanusis known to be susceptible to EBHS, but virological data available is scarce due to the few cases detected so far. In this study, we describe the occurrence of EBHS in two free-rangingL. corsicanus,found dead in a protected area of Central Italy. The two hares were identified asL. corsicanususing phenotypic criteria and confirmed through mitochondrial DNA analysis. Distinctive EBHS gross lesions were observed at necropsy and confirmed by subsequent histological examination. EBHSV was detected in the livers of the two animals initially using an antigen detection ELISA, followed by an EBHSV-specific reverse transcription-PCR, thus confirming the viral infection as the probable cause of death. The EBHS viruses detected in the two hares were identical, as based on blast analysis performed for the VP60 sequences and showed 98.86% nucleotide identity and 100% amino acid identity with strain EBHSV/GER-BY/EI97.L03477/2019, isolated in Germany in 2019. Phylogenetic analysis places our virus in group B, which includes strains that emerged after the mid-1980s. This study supports previous reports of EBHS inL. corsicanusand further expands the knowledge of the pathological and virological characteristics of the etiological agent. The ability of EBHSV to cause a fatal disease in the Italian hare represents a serious threat to the conservation of this vulnerable species, especially in populations kept in enclosed protected areas.
Characterization of the bacterial microbiome of non-hematophagous bats and associated ectoparasites from Brazil
Authors: André Marcos Rogério , Ikeda Priscila , Lee Daniel Antônio Braga , do Amaral Renan Bressianini , Carvalho Lucas Amoroso Lopes , Pinheiro Daniel Guariz , Torres Jaire Marinho , de Mello Victória Valente Califre , Rice Gregory K. , Cer Regina Z. , Lourenço Elizabete Captivo , Oliveira Carisa Elisei , Herrera Heitor Miraglia , Barros-Battesti Darci Moraes , Machado Rosangela Zacarias , Bishop-Lilly Kimberly A. , Dalgard Clifton L. , Dumler J. Stephen
Published: October 2023

Introduction

Bats, along with their ectoparasites, harbor a wide diversity of symbiotic and potential pathogenic bacteria. Despite the enormous diversity of bats (181 species), few studies aimed to investigate the bacterial microbiome of Brazilian chiropterans and associated ectoparasites. This study aimed to characterize the bacterial microbiome of non-hematophagous bats and associated Streblidae flies and Macronyssidae and Spinturnicidae mites in the state of Mato Grosso do Sul, midwestern Brazil.

Methods

Oral and rectal swabs were collected from 30 bats (Artibeus lituratus [n = 13], Artibeus planirostris [n  =  9], Eptesicus furinalis [n = 5], Carollia perspicillata [n = 2], and Platyrrhinus lineatus [n = 1]). In addition, a total of 58 mites (15 Macronyssidae and 43 Spinturnicidae) and 48 Streblidae bat flies were collected from the captured bats. After DNA extraction and purification, each sample’s bacterial composition was analyzed with metagenomic sequencing.

Results

The microbiome composition of both oral and rectal bat swab samples showed that Gammaproteobacteria was the most abundant bacterial class. Spiroplasma, Wolbachia and Bartonella represented the most abundant genera in Streblidae flies. While Wolbachia (Alphaproteobacteria) was the most abundant genus found in Spinturnicidae, Arsenophonus (Gammaproteobacteria) was found in high abundance in Macronyssidae mites. In addition to characterizing the microbiome of each sample at the class and genus taxonomic levels, we identified medically significant bacteria able to infect both animals and humans in oral (Streptococcus and Anaplasma) and rectal swabs (Enterobacter, Klebsiella, Escherichia, Enterococcus, Streptococcus), Macronyssidae (Anaplasma, Bartonella, Ehrlichia) and Spinturnicidae (Anaplasma, Bartonella) mites as well as Streblidae flies (Spiroplasma, Bartonella).

Discussion and conclusion

Besides expanding the knowledge on the bacterial microbiome of non-hematophagous bats and Streblidae flies from Brazil, the present work showed, for the first time, the bacterial community of bat-associated Macronyssidae and Spinturnicidae mites.
Screening and evaluation of skin potential probiotic from high-altitude Tibetans to repair ultraviolet radiation damage
Authors: Zhang Zhihao , Ran Haixia , Hua Yutong , Deng Feilong , Zeng Bo , Chai Jianmin , Li Ying
Published: October 2023

Human skin microbes play critical roles in skin health and diseases. Microbes colonizing on the skin of Tibetans living in the high-altitude area for generations may have a stronger ability to resist the harsh environment, such as high ultraviolet radiation (UV). Isolation of a potential probiotic from Tibetans skin is beneficial for resistance of skin disease for humans in the world. In this study, the signature microbiota for Tibetan skin were characterized compared to low-altitude humans. Next, using culture-omics, 118 species were isolated. The culturability of high-altitude of Tibetan skin microbiome reached approximate 66.8%. Next, we found that one strain, Pantoea eucrina, had the greatest ability to repair UV damage to the skin as the lowest pathological score was observed in this group. Interestingly, another animal trial found this bacterium resisted UV rather than its metabolites. Using whole genome sequencing, this strain P. eucrina KBFS172 was confirmed, and its functions were annotated. It might involve in the metabolic pathway of carotenoid biosynthesis with anti-oxidative stress properties, which plays critical roles in UV-damage repair. In conclusion, we characterized the signature microbes of skin in high-altitude Tibetans, isolated a skin bacterium of Pantoea eucrina KBFS172 which could repair UV damage via involving the metabolic pathway of carotenoid biosynthesis. Our results provide a new potential skin probiotic for skin disease prevention or sunburn.
Brain-inhabiting bacteria and neurodegenerative diseases: the “brain microbiome” theory
Authors: Arabi Tarek Ziad , Alabdulqader Aliyah Abdulmohsen , Sabbah Belal Nedal , Ouban Abderrahman
Published: October 2023

Controversies surrounding the validity of the toxic proteinopathy theory of Alzheimer’s disease have led the scientific community to seek alternative theories in the pathogenesis of neurodegenerative disorders (ND). Recent studies have provided evidence of a microbiome in the central nervous system. Some have hypothesized that brain-inhabiting organisms induce chronic neuroinflammation, leading to the development of a spectrum of NDs. Bacteria such as Chlamydia pneumoniae, Helicobacter pylori, and Cutibacterium acnes have been found to inhabit the brains of ND patients. Furthermore, several fungi, including Candida and Malassezia species, have been identified in the central nervous system of these patients. However, there remains several limitations to the brain microbiome hypothesis. Varying results across the literature, concerns regarding sample contamination, and the presence of exogenous deoxyribonucleic acids have led to doubts about the hypothesis. These results provide valuable insight into the pathogenesis of NDs. Herein, we provide a review of the evidence for and against the brain microbiome theory and describe the difficulties facing the hypothesis. Additionally, we define possible mechanisms of bacterial invasion of the brain and organism-related neurodegeneration in NDs and the potential therapeutic premises of this theory.
Nomogram model of functional outcome for endovascular treatment in patients with acute basilar artery occlusion
Authors: Li Lei , Lv Jin , Han Jian-jia , Gao Yuan , Yan Zhao-xuan , Wu Qi , Zhang Xiao-li , Gao Feng
Published: October 2023

Background and purpose

The efficacy and safety of endovascular treatment (EVT) in acute basilar artery occlusion (ABAO) has been confirmed by four randomized clinical trials. Nevertheless, the predictors of a 90-day favorable outcome after EVT have not been elucidated. We attempted to establish a nomogram for the prediction of a 90-day favorable outcome in ABAO patients with EVT.

Methods

Clinical data of ABAO patients with EVT were obtained from two nationwide clinical trial registries in China. Factors associated with a 90-day favorable outcome were screened by multivariable step-wise regression on the basis of univariable analysis. A nomogram was established to predict 90-day favorable outcome after EVT.

Results

The proportion of ABAO patients with a favorable outcome was 41.53% (157/378). Seven variables, including baseline National Institutes of Health Stroke Scale (NIHSS) <20 [odds ratio (OR): 8.330; P-value < 0.0001], posterior circulation Alberta Stroke Program Early CT (pc-ASPECT) score ≥7 (OR: 1.948; P-value = 0.0296), Pons-Midbrain Index (PMI) score < 2 (OR: 2.108; P-value = 0.0128), Posterior Circulation Collateral Score (PC-CS) ≥5 (OR: 3.288; P-value < 0.0001), local anesthesia (OR: 0.389; P-value = 0.0017), time from onset to recanalization (OTR) <330 min (OR: 2.594; P-value = 0.0013), and no occurrence of early neurological deterioration (END; OR: 0.039; P-value < 0.0001) were included into the nomogram, with C-index values of 0.8730 and 0.8857 in the training and the internal validation set, respectively.

Conclusions

The proposed nomogram provided a reliable prognostic scale, which can be employed in clinical settings for the selection and clinical management of ABAO patients.

Registration

https://www.clinicaltrials.gov, identifier: NCT03370939.
The external evocation and movement-related modulation of motor cortex inhibition in children and adolescents with Tourette syndrome – a TMS/EEG study
Authors: Schmidgen Julia , Konrad Kerstin , Roessner Veit , Bender Stephan
Published: October 2023

Objective

This study tested the reactivity of motor cortex inhibition to different intensities of external stimulation by transcranial magnetic stimulation (TMS) and its internal modulation during different motor states in children and adolescents with Tourette syndrome.

Methods

TMS-evoked N100 served as an indirect measure of GABAB receptor function which is related to cortical inhibition. Combined TMS/EEG was used to analyze the TMS-evoked N100 component evoked by different stimulation intensities as well as during resting condition, movement preparation (contingent negative variation task) and movement execution. The study included 18 early adolescents with Tourette syndrome and 15 typically developing control subjects.

Results

TMS-evoked N100 showed a less steep increase with increasing TMS intensity in Tourette syndrome together with less modulation (disinhibition) over the primary motor cortex during the motor states movement preparation and movement execution. Children with Tourette syndrome showed equally high N100 amplitudes at 110% resting motor threshold (RMT) intensity during resting condition and a parallel decline of RMT and N100 amplitude with increasing age as control subjects.

Conclusion

Our study yields preliminary evidence that modulation of motor cortical inhibitory circuits, during external direct stimulation by different TMS intensities and during volitional movement preparation and execution is different in children and adolescents with Tourette syndrome compared to controls. These results suggest that a reduced resting motor cortical inhibitory “reserve” could contribute to the production of unwanted movements. Our findings are compatible with increased regulation of motor cortex excitability by perception-action binding in Tourette syndrome instead of top-down / motor regulation and need to be replicated in further studies.
Evaluation of cellular activity in response to sleep deprivation by a comprehensive analysis of the whole mouse brain
Authors: Urushihata Takuya , Goto Mio , Kabetani Keiko , Kiyozuka Mai , Maruyama Shiho , Tsuji Shogo , Tada Hirobumi , Satoh Akiko
Published: October 2023

Sleep deprivation (SD) causes several adverse functional outcomes, and understanding the associated processes can improve quality of life. Although the effects of SD on neuronal activity in several brain regions have been identified, a comprehensive evaluation of the whole brain is still lacking. Hence, we performed SD using two different methods, gentle handling and a dedicated chamber, in targeted recombination in active populations 2 (TRAP2) mice crossed with Rosa-ZsGreen reporter mice and visualized cellular activity in the whole brain. Using the semi-automated post-imaging analysis tool Slice Histology Alignment, Registration, and Cell Quantification (SHARCQ), the number of activated cells was quantified. From the analysis of 14 brain regions, cellular activity was significantly increased in the olfactory areas and decreased in the medulla by the two SD methods. From the analysis of the further subdivided 348 regions, cellular activity was significantly increased in the vascular organ of the lamina terminalis, lateral hypothalamic area, parabigeminal nucleus, ventral tegmental area, and magnocellular reticular nucleus, and decreased in the anterior part of the basolateral amygdalar nucleus, nucleus accumbens, septohippocampal nucleus, reticular nucleus of the thalamus, preoptic part of the periventricular hypothalamic nucleus, ventromedial preoptic nucleus, rostral linear nucleus raphe, facial motor nucleus, vestibular nuclei, and some fiber tracts (oculomotor nerve, genu of corpus callosum, and rubrospinal tract) by the two SD methods. Two subdivided regions of the striatum (caudoputamen and other striatum), epithalamus, vascular organ of the lamina terminalis, anteroventral preoptic nucleus, superior colliculus optic layer, medial terminal nucleus of the accessory optic tract, pontine gray, and fiber tracts (medial lemniscus, columns of the fornix, brachium of the inferior colliculus, and mammillary peduncle) were differentially affected by the two SD methods. Most brain regions detected from these analyses have been reported to be involved in regulating sleep/wake regulatory circuits. Moreover, the results from the connectivity analysis indicated that the connectivity of cellular activity among brain regions was altered by SD. Together, such a comprehensive analysis of the whole brain is useful for understanding the mechanisms by which SD and/or sleep disruption affects brain function.
A novel approach to assess diet diversity: a development of the Nutritional Functional Diversity indicator
Authors: Di Maso Matteo , Bravi Francesca , Polesel Jerry , Dal Maso Luigino , Patel Linia , La Vecchia Carlo , Ferraroni Monica
Published: October 2023

Background and aims

Diversity is a key element of diet quality. The Food Variety Score (FVS) is used to assess diversity, especially in low- and middle-income countries. It sums up the number of foods consumed ignoring their nutrient content. A more suitable index should combine the number of foods consumed and their nutritional composition. We adapted the Nutritional Functional Diversity indicator (NFD), proposed by ecologists, to measure diversity in the human diet. We compared NFD and FVS evaluating subjects’ distributions across quartiles of the two diversity indices. To evaluate which one reflected a higher diet quality, we estimated associations between these two diversity indices and diet quality measures, i.e., the Mediterranean Diet Score (MDS) and the Healthy Eating Index-2015 (HEI-2015). Associations were expressed by odds ratios (OR) and corresponding 95% confidence intervals (CI).

Materials and methods

We used the data of controls only derived from an integrated series of hospital-based case-control cancer studies conducted in different Italian areas. The NFD identifies groups of foods based on a set of nutrients according to a cluster analysis. Some steps are required: creating a food-nutrient matrix; clustering of the Euclidean food-food distance matrix to identify groups of foods with nutritional (dis)similarities; and calculating the NFD as the ratio between the sum of branch lengths of the dendrogram belonging to the number of foods consumed by individuals (i.e., subject-specific diversity) and the sum of all branch lengths of the dendrogram (i.e., maximal diversity).

Results

More than one quarter of individuals (28.4%) were differently classified within quartiles of the two diversity indices. For both indices, increasing the diversity level increased the risk for adhering to MDS (OR for NFD = 11.26; 95% CI: 7.88–16.09, and OR for FVS = 6.80; 95% CI: 4.84–9.54) and to HEI-2015 (OR for NDF = 2.86; 95% CI: 2.39–3.42, and OR for FVS = 2.72; 95% CI: 2.27–3.26). Associations were stronger for NFD.

Conclusion

Our findings showed a greater ability of NFD to assess diet quality quantifying the degree of diversity.
Enhancement of cation exchange and glucose binding capacity, flavonoids release and antioxidant capacity of Tartary buckwheat powder with ultrafine grinding
Authors: Wang Xinhui , Zhang Xue , Zhang Dongjie
Published: October 2023

The objective of this paper was to study the effects of ultrafine grinding on the cation exchange capacity, glucose binding capacity and in vitro digestion characteristics of Tartary buckwheat powder. The results showed that the cation exchange ability and glucose binding strength of Tartary buckwheat powder, Tartary buckwheat bran powder and Tartary buckwheat core powder increased significantly with the increase of crushing frequency (20, 40 and 60 Hz), and the Tartary buckwheat bran powder was the highest. The results of in vitro digestion showed that ultrafine grinding improved the flavonoid release and antioxidant activity of Tartary buckwheat bran powder in the in vitro digestion process. The correlation analysis indicated that the amount of flavonoids released in digestive fluid was significantly related to antioxidant activity. This study may provide a theoretical basis for improving the physicochemical properties and functions of Tartary buckwheat by ultrafine grinding technology.
Fully automated 3D body composition analysis and its association with overall survival in head and neck squamous cell carcinoma patients
Authors: Rozynek Miłosz , Gut Daniel , Kucybała Iwona , Strzałkowska-Kominiak Ewa , Tabor Zbisław , Urbanik Andrzej , Kłęk Stanisław , Wojciechowski Wadim
Published: October 2023

Objectives

We developed a method for a fully automated deep-learning segmentation of tissues to investigate if 3D body composition measurements are significant for survival of Head and Neck Squamous Cell Carcinoma (HNSCC) patients.

Methods

3D segmentation of tissues including spine, spine muscles, abdominal muscles, subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT), and internal organs within volumetric region limited by L1 and L5 levels was accomplished using deep convolutional segmentation architecture - U-net implemented in a nnUnet framework. It was trained on separate dataset of 560 single-channel CT slices and used for 3D segmentation of pre-radiotherapy (Pre-RT) and post-radiotherapy (Post-RT) whole body PET/CT or abdominal CT scans of 215 HNSCC patients. Percentages of tissues were used for overall survival analysis using Cox proportional hazard (PH) model.

Results

Our deep learning model successfully segmented all mentioned tissues with Dice’s coefficient exceeding 0.95. The 3D measurements including difference between Pre-RT and post-RT abdomen and spine muscles percentage, difference between Pre-RT and post-RT VAT percentage and sum of Pre-RT abdomen and spine muscles percentage together with BMI and Cancer Site were selected and significant at the level of 5% for the overall survival. Aside from Cancer Site, the lowest hazard ratio (HR) value (HR, 0.7527; 95% CI, 0.6487-0.8735; p = 0.000183) was observed for the difference between Pre-RT and post-RT abdomen and spine muscles percentage.

Conclusion

Fully automated 3D quantitative measurements of body composition are significant for overall survival in Head and Neck Squamous Cell Carcinoma patients.
Transanal total mesorectal excision: single center study on risk factors for major complications
Authors: Xu Zhiwen , Zhu Jingtao , Bai Haoyu , Xiao Liangbin , Wang Tinghao , Lin Hexin , Hong Qingqi , You Jun
Published: October 2023

Purpose

Transanal total mesorectal excision (TaTME) as a novel surgical approach for mid and low rectal cancer has gained significant research interest in recent years. The main objective of this study is to identify the risk factors associated with major complications after TaTME and evaluate the perioperative clinical outcomes.

Methods

A retrospective analysis was performed on the clinical data of patients with mid-to-low rectal cancer who underwent TaTME surgery and were admitted to the First Affiliated Hospital of Xiamen University from January 2018 to May 2023. Univariate and multivariate regression methods were employed to analyze the risk factors influencing the occurrence of major complications (Clavien-Dindo III-V).

Results

This study included a total of 179 eligible cases, with no perioperative deaths. The overall incidence of early complications was 25.1%, with a rate of 10.1% for mild complications and 15.0% for major complications. The postoperative anastomotic leakage rate within 30 days was 6.7%. Multivariate analysis demonstrated that male (P=0.030), pathological T ≥ 3 (P=0.018) and manual anastomosis (P=0.009) were independent risk factors for the development of major complications after surgery.

Conclusion

In this study, the incidence of early complications and anastomotic leakage rate in TaTME were both relatively low. Male, pathological T stage ≥ 3 and manual anastomosis were independent risk factors for the occurrence of major complications in a cohort of patients with mid and low rectal cancer undergoing TaTME.
Desmopressin in combination with anticholinergic agents in the treatment of nocturnal enuresis: a systematic review and meta-analysis
Authors: Cai Tong , Yao Yi , Sun Weigui , Lei Peipei
Published: October 2023

Background

The desmopressin combined with anticholinergic agents for the treatment of nocturnal enuresis (NE) remains controversial. This meta-analysis assesses the efficacy and safety of desmopressin compared with desmopressin plus anticholinergic agents for the treatment of NE.

Methods

We searched MEDLINE, Embase, and Cochrane Controlled Trials Register databases for RCTs published for the treatment of NE. Systematic review was carried out using the Preferred Reporting Items for Systematic Reviews and Meta-analyses. This meta-analysis used RevMan v.5.1.0 to analyze data.

Results

Eight studies involving 600 patients (293 in the combination group and 307 in the desmopressin group) contained meaningful data. The results were as follows: after one month of treatment, compared with the desmopressin monotherapy group, the combination group was significantly better in treating NE in FR (full responders, P = 0.003), FR + PR (partial responders) (P < 0.0001), and the mean number of wet nights (P = 0.004); also, the combination group had a better effect in FR (P < 0.00001), FR + PR (P = 0.02) and the mean number of wet nights (P = 0.04) after 3 months' treatment. For side effects, combination therapy does not cause more adverse events in treating NE (P = 0.42).

Conclusions

This study elucidates that desmopressin combined with the anticholinergic agent was demonstrated to be more effective in treating NE than desmopressin monotherapy, and the anticholinergic agent does not increase the risk of adverse events (AEs).
Fitness costs of resistance to insecticides in insects
Authors: Gul Hina , Gadratagi Basana Gowda , Güncan Ali , Tyagi Saniya , Ullah Farman , Desneux Nicolas , Liu Xiaoxia
Published: October 2023

The chemical application is considered one of the most crucial methods for controlling insect pests, especially in intensive farming practices. Owing to the chemical application, insect pests are exposed to toxic chemical insecticides along with other stress factors in the environment. Insects require energy and resources for survival and adaptation to cope with these conditions. Also, insects use behavioral, physiological, and genetic mechanisms to combat stressors, like new environments, which may include chemicals insecticides. Sometimes, the continuous selection pressure of insecticides is metabolically costly, which leads to resistance development through constitutive upregulation of detoxification genes and/or target-site mutations. These actions are costly and can potentially affect the biological traits, including development and reproduction parameters and other key variables that ultimately affect the overall fitness of insects. This review synthesizes published in-depth information on fitness costs induced by insecticide resistance in insect pests in the past decade. It thereby highlights the insecticides resistant to insect populations that might help design integrated pest management (IPM) programs for controlling the spread of resistant populations.