bluuebunny/crossref_metadata_2025 huggingface dataset:
The Relationship between ROP Funds and Sustainable Development—A Case Study for Poland
Authors: Mach Łukasz , Bedrunka Karina , Dąbrowski Ireneusz , Frącz Paweł
Published: May 2021

The aim of this research is to analyse the correlation between public intervention in Poland within the Regional Operational Programmes and the key macroeconomic variables for the sustainable development of regions, i.e., the labour market, with particular emphasis on the unemployment rate and the level of employment; the average monthly remuneration; the residential construction market, with particular emphasis on the number of permits issued for the construction of apartments and the number of apartments under construction. The research and the analyses carried out on the basis of the above-mentioned aspects made it possible to indicate the relations between the studied macroeconomic indicators and the EU funds spending in Polish provinces, which will enable the implementation of the sustainable development policy. The capitals used in the research process are very important components of the region’s and country’s sustainable development. In the research, a calculation methodology was applied based on the analysis of time variability of the examined determinants, their correlation and regression relationships. The tools and methods of data analysis used allowed the quantification of the relationship between the macroeconomic determinants studied and the pace and value of payments made. The conducted analyses have shown a positive influence of the payments made in Poland within the framework of Regional Operational Programmes on selected macroeconomic indicators, i.e., regional economic and social-institutional capitals. The research results obtained may have a practical decision-making aspect for regional and national authorities responsible for the disbursement of EU funds.
Variation of Chemical Components in Sapwood, Transition Zone, and Heartwood of Dalbergia odorifera and Its Relationship with Heartwood Formation
Authors: Ma Ruoke , Liu Heng , Fu Yunlin , Li Yingjian , Wei Penglian , Liu Zhigao
Published: May 2021

Heartwood has a high economic value because of its natural durability, beautiful color, special aroma, and richness in active ingredients used in traditional Chinese medicine. However, the mechanism of heartwood formation remains unclear. Dalbergia odorifera was selected as the object of research to analyze this variation in the chemical composition of sapwood, transition zone, and heartwood as well as to elucidate the relationship between this variation and the formation of heartwood. The variation of secondary metabolites was analyzed using gas chromatography-mass spectrometry and ultra-high performance liquid chromatography–mass spectrometry, the variation of lignin was analyzed using Fourier transform infrared spectroscopy and ultraviolet visible spectrophotometry, and the variation law of mineral elements was analyzed using atomic absorption spectrophotometry. The results demonstrated that contents of characteristic secondary metabolites in Dalbergia odorifera were mainly distributed in heartwood (84.3–96.8%), increased from the outer to inner layers of the xylem, and sudden changes occurred in the transition zone (the fourth growth ring). The Dalbergia odorifera lignin can be identified as typical “syringyl–guaiacyl (S–G)” lignin, and the color darkened from the outside to the inside. The results demonstrated that there were more benzene rings and conjugated C=O structures in the heartwood. Additionally, the variation of minerals in the xylem was related to elemental types; the average concentrations of Mg, Ca, Fe and Sr were higher in the heartwood than in the sapwood, whereas the concentrations of K and Zn were higher in the sapwood than in the heartwood owing to the reabsorption of elements. The concentrations of Na and Cu were similar in the heartwood and sapwood. The composition and structural characteristics of secondary metabolites, lignin, and mineral elements in the three typical xylem regions (sapwood, transition zone and heartwood) of Dalbergia odorifera changed. The most abrupt change occurred in the narrow xylem transition zone, which is the key location involved in heartwood formation in Dalbergia odorifera.
Short-Term Impacts of Harvesting Intensity on the Upper Soil Layers in High Karst Dinaric Fir-Beech Forests
Authors: Hukić Emira , Čater Matjaž , Marinšek Aleksander , Ferlan Mitja , Kobal Milan , Žlindra Daniel , Čustović Hamid , Simončič Primož
Published: May 2021

The present study addresses the short-term effects of different harvest intensities under close-to-nature selective management on the upper soil layers in Slovenian and Bosnian Dinaric karst fir-beech forests. The different harvest intensities coincided with the single-tree and irregular shelterwood management, common in the region. The effect of harvesting intensity on the upper soil layers (Ol, Of, Ol and 0–10 cm mineral soil) was investigated by a repeated measurements experiment in Slovenia on 27 research plots in close-to nature managed forests. The properties of the upper layers (concentration of SOC and TN, C/N ratio, weights, BD and SOC stocks) were analyzed twice, before (2011) and after (2014) treatment of 50% and 100% harvest intensity in relation to the total standing growing stock of trees. As a control, we used no-treatment <20% harvesting intensity plots. To extend this experiment, we added three comparable plots from the Bosnian site: one in an old-growth forest with 0% harvest intensity and two in the managed forest with <20% harvest intensity. The results of the assessment of mean differences indicated a significant influence of harvesting intensity on the decrease in SOC, TN concentrations, weights and SOC stocks in the organic layers and the increase in BD and SOC stocks in the 0–10 cm mineral soil. The highest relative decreases in Ol, Of and Oh SOC stocks occurred in 50% (−10 and −38%) and 100% (−16 and −49%) harvest intensities. Negligible relative differences in both organic and 0–10 cm mineral layers were found for the <20% harvest intensity in the region. The change in forest light conditions resulting from differences in canopy openness as a function of applied harvest intensity explained the significant difference in the properties of the upper soil layers. The impact of the short-term losses in SOC stocks, in terms of overall soil productivity, may depend on the regeneration dynamics and melioration methods.
A Statistical Workflow to Evaluate the Modulation of Wine Metabolome and Its Contribution to the Sensory Attributes
Authors: Muñoz-Redondo José Manuel , Puertas Belén , Pereira-Caro Gema , Ordóñez-Díaz José Luis , Ruiz-Moreno María José , Cantos-Villar Emma , Moreno-Rojas José Manuel
Published: May 2021

A data-processing and statistical analysis workflow was proposed to evaluate the metabolic changes and its contribution to the sensory characteristics of different wines. This workflow was applied to rosé wines from different fermentation strategies. The metabolome was acquired by means of two high-throughput techniques: gas chromatography–mass spectrometry (GC-MS) and liquid chromatography–mass spectrometry (LC-MS) for volatile and non-volatile metabolites, respectively, in an untargeted approach, while the sensory evaluation of the wines was performed by a trained panel. Wine volatile and non-volatile metabolites modulation was independently evaluated by means of partial least squares discriminant analysis (PLS-DA), obtaining potential markers of the fermentation strategies. Then, the complete metabolome was integrated by means of sparse generalised canonical correlation analysis discriminant analysis (sGCC-DA). This integrative approach revealed a high link between the volatile and non-volatile data, and additional potential metabolite markers of the fermentation strategies were found. Subsequently, the evaluation of the contribution of metabolome to the sensory characteristics of wines was carried out. First, the all-relevant metabolites affected by the different fermentation processes were selected using PLS-DA and random forest (RF). Each set of volatile and non-volatile metabolites selected was then related to the sensory attributes of the wines by means of partial least squares regression (PLSR). Finally, the relationships among the three datasets were complementary evaluated using regularised generalised canonical correlation analysis (RGCCA), revealing new correlations among metabolites and sensory data.
Effects of Different Densities of Sea Grape Caulerpa lentillifera on Water Quality, Growth and Survival of the Whiteleg Shrimp Litopenaeus vannamei in Polyculture System
Authors: Ly Khanh Van , Murungu David Kamau , Nguyen Dung Phuong , Nguyen Ngoc Anh Thi
Published: May 2021

The integrated aquaculture-seaweed system has been identified as a bio-mitigation strategy to overcome environmental damage, improve the efficiency of nutrient use, maintain good water quality, and ensure the system’s sustainability. This study was conducted to determine the appropriate density of sea grape (Caulerpa lentillifera) in polyculture with whiteleg shrimp (Litopenaeus vannamei) in the same culture tank. Five treatments were randomly designed in triplicate tanks where shrimp was monocultured (without sea grape) as a control treatment and four polyculture treatments with different seaweed density levels (0.5, 1, 1.5, and 2 kg m−3) for 56 days. The results showed that polyculture of shrimp and sea grape significantly reduced the concentrations of total ammonia nitrogen (TAN), nitrite (NO2−), nitrate (NO3−), and phosphate (PO43−) in the rearing tanks and significantly improved (p < 0.05) the growth rate (6.67–6.76% day−1), survival (73.3–78.5%), and production of shrimp (3.44–3.87 kg m−3) compared to monoculture (6.24% day−1, 54.8%, and 2.02 kg m−3, respectively). Applying shrimp and sea grape polyculture at a density of 1 kg m−3 provided a relatively better shrimp performance and feed conversion ratio than other seaweed densities, although not significantly different among polyculture treatments. The findings suggested that sea grape could be used at densities of 0.5–2 kg m−3 in polyculture with whiteleg shrimp, of which 1 kg m−3 resulted in higher production and feed efficiency.
Exploration of Spanish Olive Oil Quality with a Miniaturized Low-Cost Fluorescence Sensor and Machine Learning Techniques
Authors: Venturini Francesca , Sperti Michela , Michelucci Umberto , Herzig Ivo , Baumgartner Michael , Caballero Josep Palau , Jimenez Arturo , Deriu Marco Agostino
Published: May 2021

Extra virgin olive oil (EVOO) is the highest quality of olive oil and is characterized by highly beneficial nutritional properties. The large increase in both consumption and fraud, for example through adulteration, creates new challenges and an increasing demand for developing new quality assessment methodologies that are easier and cheaper to perform. As of today, the determination of olive oil quality is performed by producers through chemical analysis and organoleptic evaluation. The chemical analysis requires advanced equipment and chemical knowledge of certified laboratories, and has therefore limited accessibility. In this work a minimalist, portable, and low-cost sensor is presented, which can perform olive oil quality assessment using fluorescence spectroscopy. The potential of the proposed technology is explored by analyzing several olive oils of different quality levels, EVOO, virgin olive oil (VOO), and lampante olive oil (LOO). The spectral data were analyzed using a large number of machine learning methods, including artificial neural networks. The analysis performed in this work demonstrates the possibility of performing the classification of olive oil in the three mentioned classes with an accuracy of 100%. These results confirm that this minimalist low-cost sensor has the potential to substitute expensive and complex chemical analysis.
Intended Ranges and Correlations between Percentages of Variables Like Oleic Acid, Eicosapentaenoic Acid, and Arachidonic Acid
Authors: Høstmark Arne Torbjørn
Published: May 2021

In chicken muscle, we previously showed that ranges of oleic acid (OA), arachidonic acid (AA), and eicosapentaenoic acid (EPA) might explain why %OA was inversely related to %AA, and that %EPA correlated positively with %AA. We here try to clarify further how ranges of the fatty acids could make strong associations between their relative amounts, utilizing published data from chicken muscle and human sera. We generated random number variables (OA’, AA’, EPA’) in lieu of the true variables, and we studied effects of altering their ranges upon scatterplots of %OA’ vs. %AA’ (%EPA’), and %AA’ vs. %EPA’. To explain the results, we first applied the equation OA’ + AA’ + EPA’ = S, i.e., %OA’ + %AA’ + %EPA’ = 100. Next, we considered how the OA’ (AA’, EPA’) fractions of S related to S. Increasing the OA’ range towards higher values improved the positive association between %AA’ and %EPA’. Thus, increased intake of OA could improve the positive correlations between percentages of eicosanoid precursors, raising the question of whether “intended ranges” of some fatty acids represent a case of evolutionary selection to, e.g., achieve balance between eicosanoids.
Characterization and Regulation of the Acetolactate Synthase Genes Involved in Acetoin Biosynthesis in Acetobacter pasteurianus
Authors: Zhao Jingyi , Meng Zhe , Ma Xiaolong , Zhao Shumei , An Yang , Xiao Zijun
Published: May 2021

Acetoin is an important aroma-active chemical in cereal vinegars. Acetobacter pasteurianus was reported to make a significant contribution to acetoin generation in cereal vinegars. However, the related acetoin biosynthesis mechanism was largely unknown. Two annotated acetolactate synthase (ALS) genes of A. pasteurianus were investigated in this study to analyze their functions and regulatory mechanisms. Heterologous expression in Escherichia coli revealed that only AlsS1 exhibited ALS activity and had the optimal activity at 55 °C and pH 6.5. Two alsS-defective mutants of A. pasteurianus CICC 22518 were constructed, and their acetoin yields were both reduced, suggesting that two alsS genes participated in acetoin biosynthesis. A total 79.1% decrease in acetoin yield in the alsS1-defective mutant revealed that alsS1 took a major role. The regulator gene alsR disruptant was constructed to analyze the regulation effect. The decline of the acetoin yield and down-regulation of the alsD and alsS1 gene transcriptions were detected, but the alsS2 gene transcription was not affected. Acetoin was an important metabolite of lactate catabolism in A. pasteurianus. The coexistence of two alsS genes can help strains rapidly and securely assimilate lactate to deal with the lactate pressure in a vinegar brewing environment, which represented a new genetic mode of acetoin production in bacteria.
Inhibitory Effect of Catechin-Rich Açaí Seed Extract on LPS-Stimulated RAW 264.7 Cells and Carrageenan-Induced Paw Edema
Authors: Xavier Gabriel Silva , Teles Amanda Mara , Moragas-Tellis Carla Junqueira , Chagas Maria do Socorro dos Santos , Behrens Maria Dutra , Moreira Wendel Fragoso de Freitas , Abreu-Silva Ana Lucia , Calabrese Kátia da Silva , Nascimento Maria do Desterro Soares Brandão , Almeida-Souza Fernando
Published: May 2021

Açaí berry is a fruit from the tree commonly known as açaízeiro (Euterpe oleracea Mart.) originated from the Amazonian region and widely consumed in Brazil. There are several reports of the anti-inflammatory activity of its pulp and few data about the seed’s potential in inflammation control. This work aimed to evaluate the effect of catechin-rich açaí extract on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and carrageenan-induced paw edema. The treatment with E. oleracea ethyl acetate extract (EO-ACET) was used in an in vitro model performed with macrophages stimulated by LPS, in which pro-inflammatory markers were evaluated, and in an in vivo model of acute inflammation, in which edema inhibition was evaluated. EO-ACET showed an absence of endotoxins, and did not display cytotoxic effects in RAW 264.7 cells. LPS-stimulated cells treated with EO-ACET displayed low levels of nitrite and interleukins (IL’s), IL-1β, IL-6 and IL-12, when compared to untreated cells. EO-ACET treatment was able to inhibit carrageenan-induced paw edema at 500 and 1000 mg/kg, in which no acute inflammatory reaction or low mast cell counts were observed by histology at the site of inoculation of λ-carrageenan. These findings provide more evidence to support further studies with E. oleracea seeds for the treatment of inflammation.
Effect of Farming System (Organic vs. Conventional) and Season on Composition and Fatty Acid Profile of Bovine, Caprine and Ovine Milk and Retail Halloumi Cheese Produced in Cyprus
Authors: Tzamaloukas Ouranios , Neofytou Marina C. , Simitzis Panagiotis E. , Miltiadou Despoina
Published: May 2021

The present work aimed to evaluate the effect of farming practices and season on the fat and protein content and fatty acid (FA) profile of milk and Halloumi cheese produced in Cyprus. Over a year, raw bulk-tank milk samples from cow, goat, and sheep farms were collected seasonally from all organic (11) and representative conventional (44) dairy farms, whereas Fresh Halloumi cheese samples were collected monthly from retail outlets (48 organic and 48 conventional samples in total). The different farming practices did not affect the milk fat content of ruminants, while protein levels were decreased in organic bovine and caprine milk. Under organic farming practices, milk and cheese contained increased values of total mono-unsaturated FA (MUFA) and poly-unsaturated FA (PUFA), and specific FA, such as oleic, conjugated linoleic, linoleic, and α-linolenic acids. Total saturated FA (SFA) levels were particularly decreased in organic samples and, consequently, the atherogenic indices of milk and cheese were decreased. Season influenced milk and Halloumi cheese FA profile; spring samples had lower SFA and higher PUFA and MUFA concentrations. Overall, the organic farm practices improved the lipid profile of milk and Halloumi cheese, which is more likely attributed to the different feeding strategies applied in organic dairy farms.
Features of Secular Changes in the Flux Density of the Cas a Supernova Remnant, from Centimeter to Decameter Wavelengths
Authors: Gorbynov Artem , Sukharev Artem , Ryabov Michail , Bezrukovs Vladislavs , Orbidans Arturs
Published: May 2021

The purpose of this work is to summarize a large amount of observational data about the secular decrease of Cas A flux in the radio range, as an indicator of physical processes both in the source itself and as a consequence of the influence of the propagation medium. This paper presents results of observations Cas A and Cyg A on radio telescopes of International Radio Astronomy Center (Latvia), and URAN-4 phased array antenna (Institute of Radio Astronomy NAS of Ukraine). According to URAN-4 observations, there were seasonal–diurnal changes in Cas A/Cyg A flux ratios due to the effects of solar activity on the ionosphere, and there may be no secular decrease in Cas A flux density, or a weak tendency to decrease it. The significant influence of the ionosphere makes it difficult to use Cyg A as a reference source in the decameter radio range. In the centimeter radio range, there were episodic intra-day variations at the level 8–10% of Cas A averaged flux. Additionally, in the period January–February 2021, Cas A flux was about 1.7 times that of Cyg A. Taking into account the observed complex type of secular decrease in Cas A flux against the background of changes in space weather variations, further observations of Cas A were planned at radio observatories in Latvia and Ukraine.
Fabrication and Characterization of Poly (vinyl alcohol) and Chitosan Oligosaccharide-Based Blend Films
Authors: Qureshi Dilshad , Sahoo Ayasharani , Mohanty Biswaranjan , Anis Arfat , Kulikouskaya Viktoryia , Hileuskaya Kseniya , Agabekov Vladimir , Sarkar Preetam , Ray Sirsendu Sekhar , Maji Samarendra , Pal Kunal
Published: May 2021

In the present study, we report the development of poly (vinyl alcohol) (PVA) and chitosan oligosaccharide (COS)-based novel blend films. The concentration of COS was varied between 2.5–10.0 wt% within the films. The inclusion of COS added a brown hue to the films. FTIR spectroscopy revealed that the extent of intermolecular hydrogen bonding was most prominent in the film that contained 5.0 wt% of COS. The diffractograms showed that COS altered the degree of crystallinity of the films in a composition-dependent manner. As evident from the thermal analysis, COS content profoundly impacted the evaporation of water molecules from the composite films. Stress relaxation studies demonstrated that the blend films exhibited more mechanical stability as compared to the control film. The impedance profiles indicated the capacitive-dominant behavior of the prepared films. Ciprofloxacin HCl-loaded films showed excellent antimicrobial activity against Escherichia coli and Bacillus cereus. The prepared films were observed to be biocompatible. Hence, the prepared PVA/COS-based blend films may be explored for drug delivery applications.
Nanopore Sequencing and Hi-C Based De Novo Assembly of Trachidermus fasciatus Genome
Authors: Xie Gangcai , Zhang Xu , Lv Feng , Sang Mengmeng , Hu Hairong , Wang Jinqiu , Liu Dong
Published: May 2021

Trachidermus fasciatus is a roughskin sculpin fish widespread across the coastal areas of East Asia. Due to environmental destruction and overfishing, the population of this species is under threat. In order to protect this endangered species, it is important to have the genome sequenced. Reference genomes are essential for studying population genetics, domestic farming, and genetic resource protection. However, currently, no reference genome is available for Trachidermus fasciatus, and this has greatly hindered the research on this species. In this study, we integrated nanopore long-read sequencing, Illumina short-read sequencing, and Hi-C methods to thoroughly assemble the Trachidermus fasciatus genome. Our results provided a chromosome-level high-quality genome assembly with a predicted genome size of 542.6 Mbp (2n = 40) and a scaffold N50 of 24.9 Mbp. The BUSCO value for genome assembly completeness was higher than 96%, and the single-base accuracy was 99.997%. Based on EVM-StringTie genome annotation, a total of 19,147 protein-coding genes were identified, including 35,093 mRNA transcripts. In addition, a novel gene-finding strategy named RNR was introduced, and in total, 51 (82) novel genes (transcripts) were identified. Lastly, we present here the first reference genome for Trachidermus fasciatus; this sequence is expected to greatly facilitate future research on this species.
Genetic Confirmation and Identification of Novel Variants for Glanzmann Thrombasthenia and Other Inherited Platelet Function Disorders: A Study by the Korean Pediatric Hematology Oncology Group (KPHOG)
Authors: Yang Eu , Shim Ye , Kim Heung , Lim Young , Im Ho , Koh Kyung-Nam , Kim Hyery , Suh Jin , Park Eun , Lee Na , Choi Young , Hah Jeong , Lee Jae , Han Jung , Lee Jae , Lee Young-Ho , Jung Hye , Ha Jung-Sook , Ki Chang-Seok ,   on behalf of the Benign Hematology Committee of the Korean Pediatric Hematology Oncology Group (KPHOG)
Published: May 2021

The diagnosis of inherited platelet function disorders (IPFDs) is challenging owing to the unavailability of essential testing methods, including light transmission aggregometry and flow cytometry, in several medical centers in Korea. This study, conducted by the Korean Pediatric Hematology Oncology Group from March 2017 to December 2020, aimed to identify the causative genetic variants of IPFDs in Korean patients using next-generation sequencing (NGS). Targeted exome sequencing, followed by whole-genome sequencing, was performed for diagnosing IPFDs. Of the 11 unrelated patients with suspected IPFDs enrolled in this study, 10 patients and 2 of their family members were diagnosed with Glanzmann thrombasthenia (GT). The variant c.1913+5G>T of ITGB3 was the most common, followed by c.2333A>C (p.Gln778Pro) of ITGB2B. Known variants of GT, including c.917A>C (p.His306Pro) of ITGB3 and c.2975del (p.Glu992Glyfs*), c.257T>C (p.Leu86Pro), and c.1750C>T (p.Arg584*) of ITGA2B, were identified. Four novel variants of GT, c.1451G>T (p.Gly484Val) and c.1595G>T (p.Cys532Phe) of ITGB3 and c.1184G>T (p.Gly395Val) and c.2390del (p.Gly797Valfs*29) of ITGA2B, were revealed. The remaining patient was diagnosed with platelet type bleeding disorder 18 and harbored two novel RASGRP2 variants, c.1479dup (p.Arg494Alafs*54) and c.813+1G>A. We demonstrated the successful application of NGS for the accurate and differential diagnosis of heterogeneous IPFDs.
Regulation of Vitamin C Accumulation for Improved Tomato Fruit Quality and Alleviation of Abiotic Stress
Authors: Mellidou Ifigeneia , Koukounaras Athanasios , Kostas Stefanos , Patelou Efstathia , Kanellis Angelos K.
Published: May 2021

Ascorbic acid (AsA) is an essential multifaceted phytonutrient for both the human diet and plant growth. Optimum levels of AsA accumulation combined with balanced redox homeostasis are required for normal plant development and defense response to adverse environmental stimuli. Notwithstanding its moderate AsA levels, tomatoes constitute a good source of vitamin C in the human diet. Therefore, the enhancement of AsA levels in tomato fruit attracts considerable attention, not only to improve its nutritional value but also to stimulate stress tolerance. Genetic regulation of AsA concentrations in plants can be achieved through the fine-tuning of biosynthetic, recycling, and transport mechanisms; it is also linked to changes in the whole fruit metabolism. Emerging evidence suggests that tomato synthesizes AsA mainly through the l-galactose pathway, but alternative pathways through d-galacturonate or myo-inositol, or seemingly unrelated transcription and regulatory factors, can be also relevant in certain developmental stages or in response to abiotic factors. Considering the recent advances in our understanding of AsA regulation in model and other non-model species, this review attempts to link the current consensus with novel technologies to provide a comprehensive strategy for AsA enhancement in tomatoes, without any detrimental effect on plant growth or fruit development.
Mucinous Cystic Neoplasm of Pancreas in a Pregnant Woman Presenting with Severe Anemia and Gastric Bleeding: Case Report and Review of the Literature
Authors: Farahmandi Susan , Elessawy Mohamed , Bauerschlag Dirk O. , Pecks Ulrich , Abdullazade Samir , Beckmann Jan Henrik , Heilmann Thorsten , Rumpf Anna-Lena , Maass Nicolai , Jansen Peer , Winkler Vincent
Published: May 2021

Mucinous cystic neoplasms of the pancreas are uncommon and especially their occurrence during pregnancy is an extremely rare event which necessitates an individualized and interdisciplinary management. A 33-year old woman was referred to our department during her third trimester of pregnancy (34th week of gestation) with severe anemia and tarry stools. Based on gastroscopic findings, our interdisciplinary team suspected a gastrointestinal stromal tumor and therefore indicated a prompt delivery via cesarean section completed with an oncological resection of the neoplasm. Histological examination subsequently showed a mucinous cystic neoplasm of the pancreas with no evidence of malignancy. To review the prevalence of mucinous cystic neoplasms and to discuss diagnosis and treatment during pregnancy. Moreover, we critically value the indication of preterm delivery and the oncological procedure in the perspective of outcome for mother and infant. A bleeding gastrointestinal tumor during pregnancy represents a life-threatening risk for mother and infant and requires an immediate interdisciplinary treatment. The urgency and radicality of the therapy should be adapted according to individual findings. As our patient’s tumor was suspected of having a malignant progression, an extensive surgical intervention was necessary.
Differences in the Pattern of Non-Recreational Sharing of Prescription Analgesics among Patients in Rural and Urban Areas
Authors: Markotic Filipa , Curkovic Mario , Pekez-Pavlisko Tanja , Vrdoljak Davorka , Vojvodic Zeljko , Jurisic Dinka , Puljiz Marijana , Novinscak Martina , Bonassin Karmela , Permozer Hajdarovic Snjezana , Tomicic Marion , Diminic-Lisica Ines , Fabris Ivsic Sonja , Nejasmic Danijel , Miosic Ivana , Novak Ivana , Puljak Livia
Published: May 2021

Introduction: This study aimed to analyze differences in sharing of prescription analgesics between rural and urban populations. Methods: We surveyed 1000 participants in outpatient family medicine settings in Croatia. We used a 35-item questionnaire to analyze patients’ characteristics, pain intensity, prescription analgesic sharing behavior, and perception of risks regarding sharing prescription medications. Results: Prescription analgesic sharing was significantly more frequent in the rural (64%) than in the urban population 55% (p = 0.01). Participants from rural areas more commonly asked for verbal or written information than those from urban areas when taking others’ prescription analgesics (p < 0.001) or giving such analgesics (p < 0.001). Participants from rural areas more commonly informed their physician about such behavior compared to those from urban areas (p < 0.01), and they were significantly more often asked about such behavior by their physician (p < 0.01). Perceptions about risks associated with sharing prescription medication were similar between rural and urban populations. Conclusions: There are systematic differences in the frequency of prescription analgesics and associated behaviors between patients in family medicine who live in rural and urban areas. Patients from rural areas were more prone to share prescription analgesics. Future studies should examine reasons for differences in sharing prescription analgesics between rural and urban areas.
The Effect of BCR-ABL Specific Tyrosine Kinase Inhibitors on the Thioredoxin System in Chronic Myeloid Leukemia
Authors: Clapper Erin , Di Trapani Giovanna , Tonissen Kathryn F.
Published: May 2021

Chronic myeloid leukemia (CML) is a myeloproliferative disorder that is caused by the formation of a fusion onco-protein, BCR-ABL. Since BCR-ABL plays a role in the progression of CML, the most common treatments of CML are tyrosine kinase inhibitors (TKIs) that specifically target BCR-ABL. However, resistance to TKIs is a major problem in CML treatment. A promising target in overcoming drug resistance in other cancers is the thioredoxin (TRX) system, an antioxidant system that maintains cellular redox homeostasis. The TRX system is upregulated in many cancers and this is associated with a poor prognosis. Analysis of a patient database showed that the expression of the TRX system was upregulated in CML patients compared to healthy donors. Our experiments revealed a significant link between the TRX and BCR-ABL systems since inhibition of BCR-ABL with chemical inhibitors and siRNA resulted in a decrease in the activity and expression of the TRX system in CML cells. This is notable as it shows that the TRX system may be a viable target in the treatment of CML.
A Multicomponent Program to Improve Self-Concept and Self-Esteem among Intimate Partner Violence Victims: A Study Protocol for a Randomized Controlled Pilot Trial
Authors: Sáez Gemma , López-Nuñez Carla , Carlos-Vivas Jorge , Barrios-Fernández Sabina , Rojo-Ramos Jorge , Adsuar José C. , Collado-Mateo Daniel
Published: May 2021

Background: Intimate Partner Violence (IPV) is a major public health problem that affects one-third of women aged 15 around the world. Interventions for IPV victims are essential for women’s self-esteem and self-concept recovery. This project aims to assess the effects of an eight-session multicomponent intervention program based on group psychological therapy and adventure activities in (1) self-esteem, (2) self-concept, (3) body image, (4) self-efficacy and (5) depression symptomatology in IPV victims. Methods/Design: A single-blind, randomized controlled pilot study, with experimental and control group, will be carried out. 34 IPV female victims will be recruited and equally assigned to the experimental (n = 17) or the control (n = 17) group. Primary outcome measures will include self-esteem, while secondary measures will be focused on self-concept, body image, self-efficacy, and depressive symptoms. Intention to treat and efficacy statistical analyses will be also performed. Discussion: This project will explore the effects of a new multicomponent program which includes cognitive-behavioral therapy sessions and outdoor adventure activities on affective and emotional variables, often affected in IPV victims. In addition, orientations to incorporate the main findings into the community based IPV resources and victims’ services will be provided.
The Role of Burnout in the Association between Work-Related Factors and Perceived Errors in Clinical Practice among Spanish Residents
Authors: Saavedra Rionda Isabel , Cortés-García Laura , de la Villa Moral Jiménez María
Published: May 2021

This study aimed to evaluate the prevalence of burnout syndrome in a sample of residents from different specialties, to determine the influence of work-related factors on the development of burnout, and to examine the mediating role of burnout in the relation between such work factors and perceived errors in clinical practice. A total of 237 Spanish residents participated (Mage = 28.87, SD = 3.84; 73.8% females). The Maslach Burnout Inventory and an ad hoc questionnaire were administered to assess burnout and work-related factors. Comparison analyses and mediational models were conducted. Half of the residents reported high levels of burnout (48.9%). Burnout was significantly associated with perceived errors in clinical practice. Significant differences were found between residents with lower and higher burnout levels, showing that those with higher burnout were less satisfied with the working conditions. Burnout mediated the associations between adjustment of responsibility, support among residents, satisfaction with teaching and rotations, general satisfaction, and perceived errors in the clinical practice. Adjusted levels of responsibility and workload, enhanced supervision, and more social support from colleagues predict lower levels of burnout, which may result in fewer errors in clinical practice. Consequently, such work-related factors should be taken into account as a preventive strategy for burnout and errors in the clinical practice so adequate patient care, good mental health of future specialists, and, therefore, higher quality of public health care can be ensured.
Exergames in Childhood Obesity Treatment: A Systematic Review
Authors: Valeriani Federica , Protano Carmela , Marotta Daniela , Liguori Giorgio , Romano Spica Vincenzo , Valerio Giuliana , Vitali Matteo , Gallè Francesca
Published: May 2021

In the last decade, active video games (exergames) have been proposed in obesity prevention and treatment as a potential tool to increase physical activity. This review was aimed to assess the possible role of exergames in reducing weight-related outcomes among overweight/obese children and/or adolescents. The databases PubMed, Scopus, Web of Science and SPORTDiscus were interrogated to detect controlled studies involving healthy overweight/obese children and adolescents in interventions based exclusively on exergames. Out of a total of 648 articles found, 10 met the inclusion criteria and were included in the review. The included studies differ for duration, setting and type of intervention, frequency of active game sessions, and outcomes considered. Seven out of ten studies reported better outcomes in children/adolescents involved in the interventions, with significant differences between groups in four, while three studies found better outcomes in control groups. These results suggest a possible positive effect of active video games on weight-related outcomes in obese children and adolescents. However, further research is still needed to define if they can be effectively used in childhood obesity treatment and which may be the most effective approach. The potentiality of the new digital media in this field should be explored.
Parental Age and the Risk of ADHD in Offspring: A Systematic Review and Meta-Analysis
Authors: Min Xianying , Li Chao , Yan Yan
Published: May 2021

Evidence has suggested that parental age at birth is a risk factor of offspring attention deficit/hyperactivity disorder (ADHD). We conducted a meta-analysis of observational studies investigating the association between parental age and offspring ADHD. We conducted a systematic search that followed the recommended guidelines for performing meta-analyses on PUBMED, EMBASE, and Web of Science up to 8 April 2021. We calculated pooled risk estimates from individual age with and without adjusting for possible confounding factors. Dose–response analysis for parental age and ADHD risk was performed. Eleven studies were selected in this meta-analysis, which included 111,101 cases and 4,417,148 participants. Compared with the reference points, the lowest parental age category was associated with an increased risk of ADHD in the offspring, with adjusted odds ratios (ORs) of 1.49 (95% confidence intervals (95%CI) 1.19–1.87) and 1.75 (95%CI 1.31–2.36) for the mother and father, respectively. The highest parental age was statistically insignificant, with adjusted ORs of 1.11 (95%CI 0.79–1.55) and 0.93 (95%CI 0.70–1.23) for mother and father separately. Dose–response analysis indicated a non-linear relationship of parental age with offspring ADHD, with the lowest ADHD risk at 31–35 years old. The results of this meta-analysis support an association between young parental age and the risk of ADHD. More high-quality studies are needed to establish whether the association with parental age is causal.
The Immediate Effect of Informational Manual Therapy for Improving Quiet Standing and Bodily Pain in University Population
Authors: Cabanas-Valdés Rosa , Toro-Coll Mª Dolores , Cruz-Sicilia Sara , García-Rueda Laura , Rodríguez-Rubio Pere Ramón , Calvo-Sanz Jordi
Published: May 2021

Background: The Informational Manual Therapy (IMT) is a therapeutic touch. This study aims to assess the effect of IMT on quiet standing, pain and health status in university population. Methods: An experiment was conducted on subjects utilizing a comparative paired analysis both before and after the intervention. One IMT session was performed on 57 healthy individuals aged from 18 to 65 years. The primary outcome was quiet standing assessed by the Satel 40 Hz stabilometric force platform. Secondary outcomes were bodily pain assessed by the 36-Item Short Form Survey (SF-36) and health status by EQ-5D-3L. The primary outcome was evaluated before and immediately after treatment. Results: The individuals were divided into 3 age groups, 18–35 (52.6%), 35–50 (29.8%) and 51–65 (17.6%). Statistically significant differences were immediately observed after the session ended when comparing the pre-post quiet stance scores in a number of length parameters: L, Lx, Ly and stabilometry amplitude on Y-axis with eyes open and closed. Significant differences were also found when testing bodily pain (SF-36) and anxiety (5Q-5D-3L). Conclusion: One session of IMT produced positive effects when testing quiet standing with eyes open and eyes closed, as well as a significant reduction in pain and anxiety for those tested. Further research is suggested.
Anaerobic Ammonium Oxidation Bacteria in a Freshwater Recirculating Pond Aquaculture System
Authors: Liu Xing-Guo , Wang Jie , Wu Zong-Fan , Cheng Guo-Feng , Gu Zhao-Jun
Published: May 2021

Anaerobic ammonium oxidation (anammox) is a key biochemical process to reduce nitrogen pollution in aquaculture, especially in water recirculating pond aquaculture system (RPAS). We used 16S RNA and quantified PCR to study the distribution and environmental impacts of anammox bacteria in RPAS. The results show that the anammox bacterial community distributions and diversities that are apparently unit-specific and seasonal have significant (p < 0.05) difference variation in the RPAS. Most of the anaerobic ammonium oxidation bacteria sequences (77.72%) retrieved from the RPAS belong to the Brocadia cluster. The abundance of anammox bacterial in the RPAS ranged from 3.33 × 101 to 41.84 × 101 copies per ng of DNA. The environmental parameter of temperature and nitrogen composition in water could have impacted the anammox bacterial abundance. This study provides more information on our understanding of the anammox bacteria in the RPAS, and provides an important basis for RPAS improvement and regulation.
The Comprehensive Machine Learning Analytics for Heart Failure
Authors: Guo Chao-Yu , Wu Min-Yang , Cheng Hao-Min
Published: May 2021

Background: Early detection of heart failure is the basis for better medical treatment and prognosis. Over the last decades, both prevalence and incidence rates of heart failure have increased worldwide, resulting in a significant global public health issue. However, an early diagnosis is not an easy task because symptoms of heart failure are usually non-specific. Therefore, this study aims to develop a risk prediction model for incident heart failure through a machine learning-based predictive model. Although African Americans have a higher risk of incident heart failure among all populations, few studies have developed a heart failure risk prediction model for African Americans. Methods: This research implemented the Least Absolute Shrinkage and Selection Operator (LASSO) logistic regression, support vector machine, random forest, and Extreme Gradient Boosting (XGBoost) to establish the Jackson Heart Study’s predictive model. In the analysis of real data, missing data are problematic when building a predictive model. Here, we evaluate predictors’ inclusion with various missing rates and different missing imputation strategies to discover the optimal analytics. Results: According to hundreds of models that we examined, the best predictive model was the XGBoost that included variables with a missing rate of less than 30 percent, and we imputed missing values by non-parametric random forest imputation. The optimal XGBoost machine demonstrated an Area Under Curve (AUC) of 0.8409 to predict heart failure for the Jackson Heart Study. Conclusion: This research identifies variations of diabetes medication as the most crucial risk factor for heart failure compared to the complete cases approach that failed to discover this phenomenon.
Mechanism of Myoglobin Molecule Adsorption on Silica: QCM, OWLS and AFM Investigations
Authors: Wasilewska Monika , Nattich-Rak Małgorzata , Pomorska Agata , Adamczyk Zbigniew
Published: May 2021

Adsorption kinetics of myoglobin on silica was investigated using the quartz crystal microbalance (QCM) and the optical waveguide light-mode spectroscopy (OWLS). Measurements were carried out for the NaCl concentration of 0.01 M and 0.15 M. A quantitative analysis of the kinetic adsorption and desorption runs acquired from QCM allowed to determine the maximum coverage of irreversibly bound myoglobin molecules. At a pH of 3.5–4 this was equal to 0.60 mg m−2 and 1.3 mg m−2 for a NaCl concentration of 0.01 M and 0.15 M, respectively, which agrees with the OWLS measurements. The latter value corresponds to the closely packed monolayer of molecules predicted from the random sequential adsorption approach. The fraction of reversibly bound protein molecules and their biding energy were also determined. It is observed that at larger pHs, the myoglobin adsorption kinetics was much slower. This behavior was attributed to the vanishing net charge that decreased the binding energy of molecules with the substrate. These results can be exploited to develop procedures for preparing myoglobin layers at silica substrates of well-controlled coverage useful for biosensing purposes.
Exposures in the Indoor Environment and Prevalence of Allergic Conditions in the United States of America
Authors: Gasana Janvier , Ibrahimou Boubakari , Albatineh Ahmed N. , Al-Zoughool Mustafa , Zein Dina
Published: May 2021

Our study examines the association of the presence of mildew, cockroaches, and pets in homes as well as household dust allergens with the prevalence and/or severity of allergic diseases. No study has concurrently assessed home environment exposures in relation to allergic conditions in the general US population. Data from 5409 participants from the 2005–2006 National Health and Nutrition Examination Survey (NHANES) living in their current homes for ≥one year were analyzed. Multivariate logistic regression analyses between home exposures and allergic diseases prevalence and severity were performed. In adjusted analyses, mildew was associated with higher current asthma, allergies, and allergic rhinitis prevalence; endotoxin, with higher current asthma prevalence; and dust Canis familiaris (Can f) 1, with higher allergic rhinitis prevalence. However, presence of cockroaches and dust Dermatophagoides farinae (Der f) 1 were associated, respectively, with lower current asthma and allergies prevalence. Presence of mildew, dust Der f1, Dermatophagoides pteronyssinus (Der p) 1, Felis domesticus (Fel d) 1, and endotoxin were all associated with asthma and/or wheeze severity. Non-atopic asthma was more frequent with mildew and/or musty smell dust and higher dust Fel d1 concentration, while atopic asthma was more prevalent with higher Can f1 and endotoxin concentrations in dust. This study confirms previous relationships and reports novel associations, generating hypotheses for future research.
Initiative in Work Teams: Lever between Authentic Leadership and Results
Authors: Lisbona Ana , Las Hayas Abel , Palací Francisco J. , Frese Michael
Published: May 2021

Background: The central point of this study is team initiative, and we analyzed how the theoretical model of antecedents and consequents of personal initiative contribute to explaining the relationship between team initiative and its antecedents and consequents. Authentic leadership is proposed as the antecedent, and the consequent leads to two types of outcomes, one of which is related to employee well-being, and the other is related to performance. However, little is known about what occurs in this relationship once the focus shifts to the team level. From a team perspective, with the label team initiative, we propose a collective construct defined similarly to personal initiative. This study shows the relationship between team initiative and its two consequences, team work engagement and performance, which are measured in terms of team productivity by the leader. Methods: Our model was tested in a field study with 344 employees of 79 work teams belonging to 55 organizations. Results: The analysis of the results using SEM and a regression analysis supported our main hypotheses. Conclusions: The finding that initiative is related to performance establishes the importance of initiative at the team level. It also emphasizes its impact on employee well-being through team work engagement and suggests the importance of authentic leadership.