bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Na/Al Ratio on the Extent of Alkali-Activation Reaction: Non-linearity and Diminishing Returns
Authors: Abdelrahman Omar , Garg Nishant
Published: January 2022

To address the high CO2footprint associated with cement production, many alternative, sustainable binders are now gaining worldwide attention–including alkali-activated materials. The alkali-activation reaction of metakaolin is a fairly complex process involving transformation of one amorphous reactant (precursor metakaolin) into another amorphous product or products (N-A-S-H gel and/or disordered zeolite type phases). In spite of this complexity, researchers in the past 2 decades have gained significant knowledge on the nature of this reaction at multiple scales. Understanding and developing a clear relationship between the alkalinity of the mix and the extent of reaction is of high interest for practical applications. However, detailed and thorough investigations on this important relationship are limited. Here, in this study, we address this gap by systematically investigating a series of alkali-activated materials samples with a wide range of Na/Al ratios (0.5–1.8) using seven different yet complementary analytical techniques (isothermal calorimetry, FTIR, XRD, TGA, NMR, and Raman imaging). Applied in tandem, these tools reveal a clear but non-linear relationship between the Na/Al ratio and the extent of alkali-activation reaction indicating diminishing returns at higher Na/Al ratios, where higher Na/Al ratios cause an increase in the degree of reaction until a certain point at which the increase in Na/Al ratio does not significantly affect the reaction kinetics, but may affect the gel polymerization. These findings could potentially aid decision making for commercial applications of AAMs where alkalinity of the mix is an important parameter for performance as well as safety.
Designing Structural Electrochemical Energy Storage Systems: A Perspective on the Role of Device Chemistry
Authors: Navarro-Suárez Adriana M. , Shaffer Milo S. P.
Published: January 2022

Structural energy storage devices (SESDs), designed to simultaneously store electrical energy and withstand mechanical loads, offer great potential to reduce the overall system weight in applications such as automotive, aircraft, spacecraft, marine and sports equipment. The greatest improvements will come from systems that implement true multifunctional materials as fully as possible. The realization of electrochemical SESDs therefore requires the identification and development of suitable multifunctional structural electrodes, separators, and electrolytes. Different strategies are available depending on the class of electrochemical energy storage device and the specific chemistries selected. Here, we review existing attempts to build SESDs around carbon fiber (CF) composite electrodes, including the use of both organic and inorganic compounds to increase electrochemical performance. We consider some of the key challenges and discuss the implications for the selection of device chemistries.
Criminal of Adverse Pregnant Outcomes: A Perspective From Thyroid Hormone Disturbance Caused by SARS-CoV-2
Authors: Shi Qiman , Wu Min , Chen Pei , Wei Bo , Tan Hailong , Huang Peng , Chang Shi
Published: January 2022

Nowadays, emerging evidence has shown adverse pregnancy outcomes, including preterm birth, preeclampsia, cesarean, and perinatal death, occurring in pregnant women after getting infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but the underlying mechanisms remain elusive. Thyroid hormone disturbance has been unveiled consistently in various studies. As commonly known, thyroid hormone is vital for promoting pregnancy and optimal fetal growth and development. Even mild thyroid dysfunction can cause adverse pregnancy outcomes. We explored and summarized possible mechanisms of thyroid hormone abnormality in pregnant women after coronavirus disease 2019 (COVID-19) infection and made a scientific thypothesis that adverse pregnancy outcomes can be the result of thyroid hormone disorder during COVID-19. In which case, we accentuate the importance of thyroid hormone surveillance for COVID-19-infected pregnant women.
Water and Emotion: Testing a New Approach for Monitoring Water Security Among Afar Pastoralists in Ethiopia
Authors: Hutchings Paul , Cooper Sarah , Butterworth John , Joseph Solome , Kebede Abinet , Parker Alison , Terefe Bethel , Van Koppen Barbara
Published: January 2022

Ethiopia has over 12 million pastoralists that raise livestock and move their herds in search of fresh pasture and water. This way of life is especially vulnerable to climate change as drought and shifts in seasonal rainfall patterns are changing the distribution and availability of these resources in pastoralist regions. The dynamic use of water within these settings is also not well-understood or captured by conventional water sector monitoring systems, which prevents appropriate supportive interventions and policies to be delivered. This paper presents results from a study into a new approach to measuring water security that focuses on assessing the emotional response of pastoralist populations to their water security situation. Formative research involving focus groups and interviews was followed by a survey of 148 pastoralists to assess their emotional response to different water security dimensions. The results indicate that emotional response can be used to elicit valuable insights into water security and provide a powerful complement to conventional water security monitoring techniques. Using the approach, we show a strong relationship between variation in seasonal water access and reported emotional response. Negative emotions also strongly associate with the most laborious methods of collecting water such as scoop holes and hand dug wells, whereas positive emotions were associated with access to higher quantities of water. Access to equines for carrying water was associated with more positive emotional well-being indicating a route to water security improvement in this context could be through the provision of donkeys and mules for water carrying. The paper discusses the value of using an emotion-based approach to capture experiences of water security alongside more conventional objective measures, especially among populations with water use patterns that continue to be poorly understood.
Multimodal User Feedback During Adaptive Robot-Human Presentations
Authors: Axelsson Agnes , Skantze Gabriel
Published: January 2022

Feedback is an essential part of all communication, and agents communicating with humans must be able to both give and receive feedback in order to ensure mutual understanding. In this paper, we analyse multimodal feedback given by humans towards a robot that is presenting a piece of art in a shared environment, similar to a museum setting. The data analysed contains both video and audio recordings of 28 participants, and the data has been richly annotated both in terms of multimodal cues (speech, gaze, head gestures, facial expressions, and body pose), as well as the polarity of any feedback (negative, positive, or neutral). We train statistical and machine learning models on the dataset, and find that random forest models and multinomial regression models perform well on predicting the polarity of the participants' reactions. An analysis of the different modalities shows that most information is found in the participants' speech and head gestures, while much less information is found in their facial expressions, body pose and gaze. An analysis of the timing of the feedback shows that most feedback is given when the robot makes pauses (and thereby invites feedback), but that the more exact timing of the feedback does not affect its meaning.
Threatened Plant Translocation for Mitigation: Improving Data Accessibility Using Existing Legislative Frameworks. An Australian Case Study
Authors: Doyle Chantelle A. , Pellow Belinda J. , Bell Stephen A. J. , Reynolds Deborah M. , Silcock Jennifer L. , Commander Lucy E. , Ooi Mark K. J.
Published: January 2022

Translocation of plants is used globally as a conservation action to bolster existing or establish new populations of threatened species and is usually communicated in academic publications or case studies. Translocation is also used to mitigate or offset impacts of urbanization and development but is less often publicly published. Irrespective of the motivation, conservation or mitigation, on ground actions are driven by overriding global conservation goals, applied in local or national legislation. This paper deconstructs the legislative framework which guides the translocation process in Australia and provides a case study which may translate to other countries, grappling with similar complexities of how existing legislation can be used to improve accessibility of translocation records. Each year, across Australia, threatened plants are being translocated to mitigate development impacts, however, limited publicly accessible records of their performance are available. To improve transparency and opportunities to learn from the outcomes of previous mitigation translocations, we propose mandatory recording of threatened plant translocations in publicly accessible databases, implemented as part of development approval conditions of consent. The contribution to these need not be onerous, at a minimum including basic translocation information (who, what, when) at project commencement and providing monitoring data (outcome) at project completion. These records are currently already collected and prepared for translocation proposals and development compliance reporting. Possible repositories for this information include the existing national Australian Network for Plant Conservation translocation database and existing State and Territory databases (which already require contributions as a condition of licensing requirements) with new provisions to identify and search for translocation records. These databases could then be linked to the Atlas of Living Australia and the Australian Threatened Plant Index. Once established, proposals for mitigation translocation could be evaluated using these databases to determine the viability of mitigation translocation as an offset measure and to build on the work of others to ensure better outcomes for plant conservation, where translocations occur.
Blood Pressure Load: An Effective Indicator of Systemic Circulation Status in Individuals With Acute Altitude Sickness
Authors: Chen Renzheng , Ye Xiaowei , Sun Mengjia , Yang Jie , Zhang Jihang , Gao Xubin , Liu Chuan , Ke Jingbin , He Chunyan , Yuan Fangzhengyuan , Lv Hailin , Yang Yuanqi , Cheng Ran , Tan Hu , Huang Lan
Published: January 2022

Background: Acute high altitude (HA) exposure results in blood pressure (BP) variations in most subjects. Previous studies have demonstrated that higher BP is potentially correlated with acute mountain sickness (AMS). The BP load may be of clinical significance regarding systemic circulation status.

Objectives: This study aimed to examine HA-induced BP changes in patients with AMS compared to those in healthy subjects. Further, we provided clinical information about the relationship between variations in 24-h ambulatory parameters (BP level, BP variability, and BP load) and AMS.

Methods: Sixty-nine subjects were enrolled and all participants ascended Litang (4,100 m above sea level). They were monitored using a 24-h ambulatory blood pressure device and underwent echocardiography within 24 h of altitude exposure. The 2018 Lake Louise questionnaire was used to evaluate AMS.

Results: The AMS group comprised more women than men [15 (65.2%) vs. 13 (28.3%), P < 0.001] and fewer smokers [4 (17.4%) vs. 23 (50.0%), P = 0.009]. The AMS group exhibited significant increases in 24-h BP compared to the non-AMS group (24-h SBP variation: 10.52 ± 6.48 vs. 6.03 ± 9.27 mmHg, P = 0.041; 24-h DBP variation: 8.70 ± 4.57 vs. 5.03 ± 4.98 mmHg, P = 0.004). The variation of mean 24-h cBPL (cumulative BP load) (mean 24-h cSBPL: 10.58 ± 10.99 vs. 4.02 ± 10.58, P = 0.016; 24-h mean cDBPL: 6.03 ± 5.87 vs. 2.89 ± 4.99, P = 0.034) was also obviously higher in AMS subjects than in non-AMS subjects after HA exposure. 24-h mean cSBPL variation (OR = 1.07, P = 0.024) and 24-h mean cDBPL variation (OR = 1.14, P = 0.034) were independent risk factors of AMS. Moreover, variation of 24-h mean cSBPL showed a good correlation with AMS score (R = 0.504, P < 0.001).

Conclusions: Our study demonstrated that patients with AMS had higher BP and BP load changes after altitude exposure than healthy subjects. Excessive BP load variations were associated with AMS. Thus, BP load could be an effective indicator regarding systemic circulation status of AMS.
MEF2A Is the Trigger of Resveratrol Exerting Protection on Vascular Endothelial Cell
Authors: Liu Benrong , Pang Lihua , Ji Yang , Fang Lei , Tian Chao Wei , Chen Jing , Chen Changnong , Zhong Yun , Ou Wen-Chao , Xiong Yujuan , Liu Shi Ming
Published: January 2022

Both resveratrol and myocyte enhancer factor 2A (MEF2A) may protect vascular endothelial cell (VEC) through activating the expression of SIRT1. However, the relationship between resveratrol and MEF2A is unclear. We aimed to investigate the deeper mechanism of resveratrol in protecting vascular endothelial cells and whether MEF2A plays a key role in the protective function of resveratrol. Human umbilical vein endothelial cell (HUVEC) was used for in vitro study, and small interfere RNA was used for silencing MEF2A. Silencing MEF2A in the vascular endothelium (VE) of ApoE−/− mice was performed by tail injection with adeno associated virus expressing si-mef2a-shRNA. The results showed that treatment of HUVEC with resveratrol significantly up-regulated MEF2A, and prevented H2O2-induced but not siRNA-induced down-regulation of MEF2A. Under various experimental conditions, the expression of SIRT1 changed with the level of MEF2A. Resveratrol could rescue from cell apoptosis, reduction of cell proliferation and viability induced by H2O2, but could not prevent against that caused by silencing MEF2A with siRNA. Silencing MEF2A in VE of apoE−/− mice decreased the expression of SIRT1, increased the plasma LDL-c, and abrogated the function of resveratrol on reducing triglyceride. Impaired integrity of VE and aggravated atherosclerotic lesion were observed in MEF2A silenced mice through immunofluorescence and oil red O staining, respectively. In conclusion, resveratrol enhances MEF2A expression, and the upregulation of MEF2A is required for the endothelial protective benefits of resveratrol in vitro via activating SIRT1. Our work has also explored the in vivo relevance of this signaling pathway in experimental models of atherosclerosis and lipid dysregulation, setting the stage for more comprehensive phenotyping in vivo and further defining the molecular mechanisms.
The Emerging Role of Rho Guanine Nucleotide Exchange Factors in Cardiovascular Disorders: Insights Into Atherosclerosis: A Mini Review
Authors: Li Mengqi , Jiao Qingzheng , Xin Wenqiang , Niu Shulin , Liu Mingming , Song Yanxin , Wang Zengguang , Yang Xinyu , Liang Degang
Published: January 2022

Atherosclerosis is a leading cause of cardiovascular disease, and atherosclerotic cardiovascular disease accounts for one-third of global deaths. However, the mechanism of atherosclerosis is not fully understood. It is well-known that the Rho GTPase family, especially Rho A, plays a vital role in the development and progression of arteriosclerosis. Rho guanine nucleotide exchange factors (Rho GEFs), which act upstream of Rho GTPases, are also involved in the atheromatous pathological process. Despite some research on the role of Rho GEFS in the regulation of atherosclerosis, the number of studies is small relative to studies on the essential function of Rho GEFs. Some studies have preliminarily revealed Rho GEF regulation of atherosclerosis by experiments in vivo and in vitro. Herein, we review the advances in research on the relationship and interaction between Rho GEFs and atheroma to provide a potential reference for further study of atherosclerosis.
Electrocardiographic and Echocardiographic Insights From a Prospective Registry of Asian Elite Athletes
Authors: Yeo Tee Joo , Wang Mingchang , Grignani Robert , McKinney James , Koh Lay Pheng , Tan Frankie Hun Yau , Chan Gregory Chung Tsing , Tay Nigel , Chan Siew-Pang , Lee Chi-Hang , Oxborough David , Malhotra Aneil , Sharma Sanjay , Richards Arthur Mark
Published: January 2022

Background:Asian representation in sport is increasing, yet there remains a lack of reference values for the Asian athlete's heart. Consequently, current guidelines for cardiovascular screening recommend using Caucasian athletes' norms to evaluate Asian athletes. This study aims to outline electrocardiographic and echocardiographic characteristics of the Asian athlete's heart using a Singaporean prospective registry of Southeast (SE) Asian athletes.

Methods and Results:One hundred and fifty elite athletes, mean age of 26.1 ± 5.7 years (50% males, 88% Chinese), were evaluated using a questionnaire, 12-lead electrocardiogram (ECG) and transthoracic echocardiogram. All ECGs were analyzed using the 2017 International Recommendations. Echocardiographic data were presented by gender and sporting discipline. The prevalence of abnormal ECGs among SE Asian athletes was 6.7%—higher than reported figures for Caucasian athletes. The abnormal ECGs comprised mainly anterior T wave inversions (ATWI) beyond lead V2, predominantly in female athletes from mixed/endurance sport (9.3% prevalence amongst females). None had echocardiographic structural abnormalities. Male athletes had reduced global longitudinal strain compared to females (−18.7 ± 1.6 vs. −20.7 ± 2.1%,p< 0.001). Overall, SE Asian athletes had smaller left ventricular cavity sizes and wall thickness compared to non-Asian athletes.

Conclusion:SE Asian athletes have higher abnormal ECG rates compared to Caucasian athletes, and also demonstrate structural differences that should be accounted for when interpreting their echocardiograms compared to athletes of other ethnicities.
Experimental Research of Compound Monitoring on Multiple Temporary Blocking Refracturing for Long-Section Horizontal Wells
Authors: Zhang Jingchen , Wu Baocheng , Wang Fei , Shi Shanzhi , Liu Jinjun , Wang Mingxing , Li Jie , Osuji Nicholas Izuchukwu
Published: January 2022

As an important energy replacement block in China, the tight conglomerate oilfields in the Mahu area are difficult to develop and are characterized by strong heterogeneity, large horizontal stress differences, and undeveloped natural fractures. However, new development processes including temporary blocking diversion and large section-multiple clusters have been implemented on the oilfields in the past few years. In 2020, two adjacent horizontal wells in the MD well area experienced a poor fracturing development effect compared with the earlier wells in this area. Analysis suggests that the main reasons are water sensitivity of the reservoir, insufficient fracturing scale, and/or interference from the adjacent old wells. To ameliorate the problem, this study presents an experimental study of multiple temporary plugging and refracturing technology in long horizontal well sections, in combination with electromagnetic and microseismic monitoring. Results from the study show a great difference between the two monitoring techniques, which is attributed to their different detection principles. Interestingly, the combination of the two approaches provides a greater performance than either approach alone. As the fracturing fluid flow diversion is based on temporary plugging diversion and electromagnetic monitoring of fracturing fluid is advantageous in temporary plugging diversion monitoring, both approaches require further research and development to address complex situations such as multiple temporary plugging and refracturing in long intervals of adjacent older wells.
Sedimentary and Source-to-Sink Evolution of Intracontinental Basins: Implications for tectonic and Climate Evolution in the Late Mesozoic (Southern Junggar Basin, NW China)
Authors: Guan Xutong , Wu Chaodong , Zhang Xuecai , Jia Weiwei , Zhang Wei
Published: January 2022

Sedimentary investigations, petrography, heavy mineral and conglomerate component analyses, and detrital zircon U-Pb geochronology were conducted to reconstruct the sedimentary and source-to-sink evolution of the Southern Junggar Basin, an intracontinental basin in the late Mesozoic. A paludal deltaic environment evolved into a fluvial environment, and abruptly prograded into alluvial fan and aeolian environments in the Late Jurassic, which was replaced by fan deltaic and lacustrine environments in the Early Cretaceous. Three source-to-sink systems were identified, according to different source-to-sink system features. In the northern piedmont of the Tianshan Orogenic Belt, the North Tianshan Orogenic Belt mainly provided sediments in the Late Jurassic. The North Tianshan and Central Tianshan Orogenic Belt both supplied sediments in the Early Cretaceous. In the northern piedmont of the Bogda Orogenic Belt, the Bogda Orogenic Belt was constantly the primary provenance, and the Tianshan Orogenic Belt also provided sediments. Sediment recycling occurred in the basin margin in the Late Jurassic and more metamorphic rocks were denudated in the Early Cretaceous. The source-to-sink system shrank in the Late Jurassic and expanded in the Early Cretaceous. This source-to-sink evolution and the conglomerates in the Kalazha Formation with seismite structures responded to the aridification in the Late Jurassic, the uplift of the Bogda and Tianshan Orogenic Belts in the Late Jurassic, and the exhumation of the Bogda and Tianshan Orogenic Belts in the Early Cretaceous.
Primary Hepatoid Adenocarcinoma of Gallbladder With MB21D2/GALNT12/ARID2 Mutations: A Case Report
Authors: Li Zhenyu , Jiang Qingming , Chen Xinyu , Xiao Yu , Xiao Jue
Published: January 2022

Background

Primary hepatoid adenocarcinoma of the gallbladder is a relatively rare type of extrahepatic adenocarcinoma. The genetic changes involved in this type of adenocarcinoma were unexplained so far. We reported a rare case of primary hepatoid adenocarcinoma of gallbladder with Mab-21 domain containing 2 (MB21D2), polypeptide N-acetylgalactosaminyltransferase 12 (GALNT12), and AT-rich interaction domain 2 (ARID2) mutations, which was confirmed after surgical resection pathologically.

Case Summary

A 69-year-old female with distention of hypogastrium and constipation received enema treatment, but ineffectively. No abnormalities were found on relevant physical examination. Then, the CT and MRI demonstrated a 3.3–4-cm soft tissue mass shadow in the neck of the gallbladder. The primary lesions consisted of two components: high-grade intraepithelial neoplasia of glands and hepatoid glands microscopically after laparoscope cholecystectomy. Immunohistochemical staining showed the sameness and difference of the two areas. Furthermore, tumor mutational burden (TMB) shows that the MB21D2, GALNT12, and ARID2 genes were mutated.

Conclusion

This is the first report of primary hepatoid adenocarcinoma of the gallbladder with MB21D2, GALNT12, and ARID2 mutations. This will provide a theoretical basis for genetic changes in rare tumors.
Hyperglycemia Is Not Associated With Higher Volumetric BMD in a Chinese Health Check-up Cohort
Authors: Wang Ling , Zhao Kaiping , Zha Xiaojuan , Ran Limei , Su Heng , Yang Yingying , Shuang Qing , Liu Yandong , Xu Li , Blake Glen M. , Cheng Xiaoguang , Engelke Klaus , Vlug Annegreet
Published: January 2022

Background and Purpose

Type 2 diabetes mellitus patients have an increased fracture risk despite having higher areal bone mineral density (aBMD) measured by DXA. This apparent paradox might be explained by the overestimation of BMD by DXA due to the higher fat mass in type 2 diabetes mellitus patients. Volumetric BMD (vBMD) as assessed by quantitative CT (QCT) is not influenced by fat mass. We assessed the association of vBMD and fasting plasma glucose in a large cohort of Chinese subjects and compared the vBMD in healthy and diabetic subjects. In addition, we compared the relation between aBMD, vBMD, glucose and fat mass in a subset of this cohort.

Materials and Methods

10309 participants from the China Biobank project underwent QCT based on chest low dose CT to compute vBMD of L1 and L2 vertebrae and FPG measurements between 2018 and 2019. Among them, 1037 subjects also had spine DXA scans. Data was analyzed using linear regression models.

Results

In the total cohort (5889 men and 4420 women, mean age 53 years, range 30-96), there was no significant association between vBMD and FPG after adjustment for age (women: p=0.774; men: p=0.149). 291 women and 606 men fitted the diagnostic criteria of diabetes. Both women and men with diabetes had lower vBMD compared to non-diabetic subjects, but this became non-significant after adjusting for age in the total cohort (women: p=0.817; men: p=0.288) and after propensity score matching based on age (women: p=0.678; men: p=0.135). In the DXA subcohort, aBMD was significantly higher in men with diabetes after adjusting for age and this difference disappeared after further adjusting for total fat area (p=0.064).

Conclusion

We did not find any effect of fasting plasma glucose or diabetes on the volumetric BMD measured with QCT after adjustment for age. Therefore, vBMD measured with QCT might be a more reliable measurement to diagnose osteoporosis and assess fracture risk than aBMD measured with DXA in diabetic patients.
Psychiatric Comorbidities and Liver Injury Are Associated With Unbalanced Plasma Bile Acid Profile During Methamphetamine Withdrawal
Authors: Ma Yuru , Wu Hongjin , Wang Huawei , Chen Fengrong , Xie Zhenrong , Zhang Zunyue , Peng Qingyan , Yang Jiqing , Zhou Yong , Chen Cheng , Chen Minghui , Zhang Yongjin , Yu Juehua , Wang Kunhua
Published: January 2022

Background

The pathogenesis of methamphetamine usedisorders (MUDs) remains largely unknown; however, bile acids may play arole as potential mediators of liver injury and psychiatric comorbidities.The aim of this study was to characterize bile acid (BA) profiles in plasmaof patients with MUDs undergoing withdrawal.

Methods

Liver functions and psychiatric symptoms wereevaluated in a retrospective cohort (30 MUDs versus 30 control subjects) andan exploratory cohort (30 MUDs including 10 subjects each at the 7-day,3-month, and 12-month withdrawal stages versus 10 control subjects). BAcompositions in plasma samples from MUD patients in the exploratory cohortwere determined by gas-liquid chromatography.

Results

Both psychiatric comorbidities andmethamphetamine-induced liver injury were observed in patients in both MUDcohorts. The plasma concentrations of the total BA, cholic acid (CA), andchenodeoxycholic acid (CDCA) were lower in MUD patients relative tocontrols. The maximum decline was observed at the 3-month stage, withgradual recovery at the 12-month stage. Notably, the ratios of deoxycholicacid (DCA)/CA and lithocholic acid (LCA)/CDCA were statistically significantat the 3-month stage comparing with controls. Significant correlations werefound between the LCA/CDCA and taurolithocholic acid (TLCA)/CDCA ratios andthe levels of alanine transaminase and aspartate aminotransferase, andbetween the LCA/CDCA ratio and the HAM-A score.

Conclusion

BA profile during METH withdrawal weremarkedly altered, with these unbalanced BAs being associated with liverinjury. The associations between BA profiles and psychiatric symptomssuggest an association between specific BAs and disease progression,possibly through the liver-brain axis.
Short-Lived Interfaces in Energy Materials
Authors: Borgschulte Andreas , Terreni Jasmin , Fumey Benjamin , Sambalova Olga , Billeter Emanuel
Published: January 2022

The kinetics of most chemical energy storage/conversion systems depend on the mass transport through matter, which is rate-limited by various kinetic barriers. The distinction of the barriers by static and dynamic interfaces helps in reducing their impact and therefore enhancing the overall kinetics. The concept is introduced along examples of static and dynamic interfaces in hydrogen storage, thermal energy storage in absorptive media, and electrochemical water splitting and CO2 reduction. In addition to the description of analysis methods to probe static and dynamic interfaces, the general strategy as well as concrete examples to overcome them are discussed.
An Optimization-Based Method for the Explicit Production Data Analysis of Gas Wells
Authors: Zhang Lixia , Li Yong , Song Xinmin , Wang Mingxian , Yu Yang , He Yingxu , Zhao Zeqi , Liu Chenchao
Published: January 2022

This work aims at the exploration of production data analysis (PDA) methods without iterations. It can overcome limitations of the advanced type curve analysis relying on the iterative calculation of material-balance pseudotime and current explicit methods reckoning on specific production schedule assumptions. The dynamic material balance equation (DMBE) is strictly proved by the integral variable substitution based on the gas flow equation under the boundary dominated flow (BDF) condition and the static material balance equation (SMBE) of a gas reservoir. We introduce the pseudopressure level functionγ(p) and the recovery factor functionR(p) to rewrite the DMBE in terms of observed variableYand estimated variableYe; then the PDA can be transformed into an optimization problem of minimizing the error betweenYandYe. An optimization-based method for the explicit production data analysis of gas wells (OBM-EPDA), therefore, is developed in the paper, capable of determining the BDF constant and gas reserves explicitly and accurately for variable rate and/or variable flowing pressure systems. Three stimulated cases demonstrate the applicability and validity of OBM-EPDA with small errors less than 1% for estimated values of both reserves andY. Not second to previous studies, the field case analysis further proves its practicability. It is shown that the nonlinear relation ofγtoRcan be represented by a polynomial function merely dependent on the inherent properties of the gas production system even before sorting out the production data. The errors of observed variableYprovided by OBM-EPDA facilitate the data quality control, and the elimination of outliers not subject to the BDF condition improves the reliability of the analysis. For various gas systems producing whether at a constant rate, a constant bottomhole pressure (BHP), or under variable rate and variable BHP conditions, the proposed method gives insights into the well-controlled volume and production capacity of the gas well whether in a low-pressure or high-pressure gas reservoir, where the compressibilities of rock and bound water are considered.
Fabrication of β-Ni(OH)2 Particles by Alkaline Etching Layered Double Hydroxides Precursor for Supercapacitor
Authors: Chen Lumei , Yang Xiaotong , Tian Ye , Wang Yiping , Zhao Xuhui , Lei Xiaodong , Zhang Fazhi
Published: January 2022

We report the facile preparation of β-Ni(OH)2 particles by etching a NiAl-layered double hydroxides (NiAl-LDHs) precursor with KOH solution. The amphoteric Al3+ ions in LDHs crystal were selectively dissolved out by KOH solution and LDHs crystals were proposed to be in situ topologically transformed to form β-Ni(OH)2. Alkaline concentration has a great influence on the structure, morphology, specific surface area, and porous structure of the resulting samples. Compared to LDHs precursor and β-Ni(OH)2 prepared by a precipitation reaction, the sample etched in 10 M KOH solution has enhanced specific capacitance (829 F/g at 1 A/g), high rate capability (capacitance retention 57.3% with current density 8 A/g), and good charge/discharge stability. We suggested that the high accessible specific surface area and appropriate porous structure, which is conducive to full contact between active material and electrolyte, can improve the utilization rate of the active material to increase the rate capacity of the 10 M KOH-etched sample.
Enterprise Innovation, Executive Experience and Internationalization Strategy: Evidence From High-Carbon Industrial Enterprises Versus Low-Carbon Industrial Enterprises in China
Authors: Cui Shuangchang
Published: January 2022

Industrial enterprises are the core subjects to reduce carbon emissions. Their innovations for low-carbon production are the key to determine the effect of carbon emission reduction. This paper examines the impact of executive experience, especially the overseas experience, on enterprise innovations across 3559 enterprises in low-carbon, medium-carbon and high-carbon industries respectively. Interestingly, it shows that the executive experience has only played a significant role in enterprise innovations of high-carbon industrial enterprises, indicating that the executive’s international vision might help to promote innovation in high-carbon industry. Then, it’s also discovered that there is a mediating effect of international strategy which helps to better understand the impact mechanism of executive experience on enterprise innovation in high-carbon industry.
Fossil Leaves of Meliosma (Sabiaceae) With Associated Pollen and a Eupodid Mite From the Eocene of Maoming Basin, South China
Authors: Moiseeva Maria G. , Kodrul Tatiana M. , Tekleva Maria V. , Maslova Natalia P. , Wu Xinkai , Jin Jianhua
Published: January 2022

A first occurrence of the genusMeliosma(Sabiaceae) is reported from the upper Eocene of the Maoming Basin of South China. This fossil is one of the oldest reliable records of the genus within its modern center of diversity. Fossil leaves are assigned to a new species,Meliosma eosinicasp. nov. based on leaf morphology and epidermal characters. The leaf epidermal anatomy of fossilMeliosmais illustrated for the first time. We also provide the first SEM observation of pollen grains associated withMeliosma. This study also documents an occurrence of mites within the leaf domatia previously unknown from the fossil record. We presume that the studied mite belongs to the superfamily Eupodoidea (Arthropoda), and probably the family Eupodidae, which comprises very small soft-bodied cosmopolitan mites occupying a wide range of terrestrial habitats. Additionally, we analyze the damage types on the fossil leaves ofMeliosma. They exhibit exclusively external foliage feeding damage caused by arthropods and traces of probable fungal infection. A review of currently known fossil occurrences of leaves, fruits, and wood ofMeliosmaprovides evidence for the geological and geographical distribution of the genus.
Phenotypic Diversity and Association Mapping of Ascorbic Acid Content in Spinach
Authors: Rueda Dario , Awika Henry O. , Bedre Renesh , Kandel Devi R. , Mandadi Kranthi K. , Crosby Kevin , Avila Carlos A.
Published: January 2022

Ascorbic acid (AsA), or vitamin C, is an essential nutrient for humans. In plants, AsA functions as an antioxidant during normal metabolism or in response to stress. Spinach is a highly nutritious green leafy vegetable that is consumed fresh, cooked or as a part of other dishes. One current goal in spinach breeding programs is to enhance quality and nutritional content. However, little is known about the diversity of nutritional content present in spinach germplasm, especially for AsA content. In this study, a worldwide panel of 352 accessions was screened for AsA content showing that variability in spinach germplasm is high and could be utilized for cultivar improvement. In addition, a genome-wide association study for marker-trait association was performed using three models, and associated markers were searched in the genome for functional annotation analysis. The generalized linear model (GLM), the compressed mixed linear model (CMLM) based on population parameters previously determined (P3D) and the perMarker model together identified a total of 490 significant markers distributed across all six spinach chromosomes indicating the complex inheritance of the trait. The different association models identified unique and overlapping marker sets, where 27 markers were identified by all three models. Identified high AsA content accessions can be used as parental lines for trait introgression and to create segregating populations for further genetic analysis. Bioinformatic analysis indicated that identified markers can differentiate between high and low AsA content accessions and that, upon validation, these markers should be useful for breeding programs.
Characterization of XR_311113.2 as a MicroRNA Sponge for Pre-ovulatory Ovarian Follicles of Goats via Long Noncoding RNA Profile and Bioinformatics Analysis
Authors: Tao Hu , Yang Juan , Zhang Pengpeng , Zhang Nian , Suo Xiaojun , Li Xiaofeng , Liu Yang , Chen Mingxin
Published: January 2022

Long noncoding RNAs (lncRNAs) were identified recently as a large class of noncoding RNAs (ncRNAs) with a length ≥200 base pairs (bp). The function and mechanism of lncRNAs have been reported in a growing number of species and tissues. In contrast, the regulatory mechanism of lncRNAs in the goat reproductive system has rarely been reported. In the present study, we sequenced and analyzed the lncRNAs using bioinformatics to identify their expression profiles. As a result, 895 lncRNAs were predicted in the pre-ovulatory ovarian follicles of goats. Eighty-eight lncRNAs were differentially expressed in the Macheng black goat when compared with Boer goat. In addition, the lncRNA XR_311113.2 acted as a sponge of chi-miR-424-5p, as assessed via a luciferase activity assay. Taken together, our findings demonstrate that lncRNAs have potential effects in the ovarian follicles of goats and may represent a promising new research field to understand follicular development.
Identification and Validation of Immune-Related Prognostic Genes in the Tumor Microenvironment of Colon Adenocarcinoma
Authors: Pan Shenghua , Tang Tingting , Wu Yanke , Zhang Liang , Song Zekai , Yu Sisi
Published: January 2022

The tumor microenvironment (TME) has been shown to be involved in angiogenesis, tumor metastasis, and immune response, thereby affecting the treatment and prognosis of patients. This study aims to identify genes that are dysregulated in the TME of patients with colon adenocarcinoma (COAD) and to evaluate their prognostic value based on RNA omics data. We obtained 512 COAD samples from the Cancer Genome Atlas (TCGA) database and 579 COAD patients from the independent dataset (GSE39582) in the Gene Expression Omnibus (GEO) database. The immune/stromal/ESTIMATE score of each patient based on their gene expression was calculated using the ESTIMATE algorithm. Kaplan–Meier survival analysis, Cox regression analysis, gene functional enrichment analysis, and protein–protein interaction (PPI) network analysis were performed. We found that immune and stromal scores were significantly correlated with COAD patients’ overall survival (log rank p < 0.05). By comparing the high immune/stromal score group with the low score group, we identified 688 intersection differentially expressed genes (DEGs) from the TCGA dataset (663 upregulated and 25 downregulated). The functional enrichment analysis of intersection DEGs showed that they were mainly enriched in the immune process, cell migration, cell motility, Toll-like receptor signaling pathway, and PI3K-Akt signaling pathway. The hub genes were revealed by PPI network analysis. Through Kaplan–Meier and Cox analysis, four TME-related genes that were significantly related to the prognosis of COAD patients were verified in GSE39582. In addition, we uncovered the relationship between the four prognostic genes and immune cells in COAD. In conclusion, based on the RNA expression profiles of 1091 COAD patients, we screened four genes that can predict prognosis from the TME, which may serve as candidate prognostic biomarkers for COAD.
EDNRA Gene rs1878406 Polymorphism is Associated With Susceptibility to Large Artery Atherosclerotic Stroke
Authors: Wei Wan , Xuan Xianjun , Zhu Jiahui , Chen Tianwen , Fang Yudan , Ding Jiao , Ji Danfei , Zhou Guoyi , Tang Bo , He Xudong
Published: January 2022

Objective: We performed this study to investigate whether the EDNRA gene rs1878406 C > T polymorphism is associated with risk of large artery atherosclerosis (LAA) stroke in the Chinese Han population.

Methods: Genotyping of rs1878406 was performed in 1,112 LAA stroke patients and 1,192 healthy controls. Multivariate logistic regression analyses were applied to assess the effect of the rs1878406 C > T polymorphism on susceptibility to LAA stroke.

Results: A significant increase of LAA stroke risk was found in the recessive model (TT vs. CC/TC, OR = 1.74, 95% CI = 1.23–2.48, p = 0.002) and co-dominant model (TC vs. CC, OR = 1.06, 95% CI = 0.89–1.27, TT vs. CC, OR = 1.79, 95% CI = 1.25–2.55, p = 0.006). However, the interaction between age and genotypes of rs1878406 was not statistically significant, and no significant interactive effect was observed between the rs1878406 C > T polymorphism and sex (p > 0.05).

Conclusion: The rs1878406 C > T polymorphism is associated with increased risk of LAA stroke in the Chinese Han population.
Machine Learning Identifies Six Genetic Variants and Alterations in the Heart Atrial Appendage as Key Contributors to PD Risk Predictivity
Authors: Ho Daniel , Schierding William , Farrow Sophie L. , Cooper Antony A. , Kempa-Liehr Andreas W. , O’Sullivan Justin M.
Published: January 2022

Parkinson’s disease (PD) is a complex neurodegenerative disease with a range of causes and clinical presentations. Over 76 genetic loci (comprising 90 SNPs) have been associated with PD by the most recent GWAS meta-analysis. Most of these PD-associated variants are located in non-coding regions of the genome and it is difficult to understand what they are doing and how they contribute to the aetiology of PD. We hypothesised that PD-associated genetic variants modulate disease risk through tissue-specific expression quantitative trait loci (eQTL) effects. We developed and validated a machine learning approach that integrated tissue-specific eQTL data on known PD-associated genetic variants with PD case and control genotypes from the Wellcome Trust Case Control Consortium. In so doing, our analysis ranked the tissue-specific transcription effects for PD-associated genetic variants and estimated their relative contributions to PD risk. We identified roles for SNPs that are connected with INPP5P, CNTN1, GBA and SNCA in PD. Ranking the variants and tissue-specific eQTL effects contributing most to the machine learning model suggested a key role in the risk of developing PD for two variants (rs7617877 and rs6808178) and eQTL associated transcriptional changes of EAF1-AS1 within the heart atrial appendage. Similarly, effects associated with eQTLs located within the Brain Cerebellum were also recognized to confer major PD risk. These findings were replicated in two additional, independent cohorts (the UK Biobank, and NeuroX) and thus warrant further mechanistic investigations to determine if these transcriptional changes could act as early contributors to PD risk and disease development.
B Cell Receptor Signaling and Protein Kinase D2 Support Regulatory B Cell Function in Pancreatic Cancer
Authors: Michaud Daniel , Mirlekar Bhalchandra , Steward Colleen , Bishop Gail , Pylayeva-Gupta Yuliya
Published: January 2022

B cells can act as potent suppressors of anti-tumor T cell immunity, presenting a mechanism of resistance to immunotherapy. In pancreatic ductal adenocarcinoma, B cells can display a T cell-suppressive or regulatory phenotype centered on the expression of the cytokine Interleukin 35 (IL-35). While B cell-mediated immunosuppression presents a barrier to anti-tumorigenic T cell function, it is not clear how regulatory B cell function could be targeted, and the signals that promote this suppressive phenotype in B cells are not well understood. Here we use a novel IL-35 reporter model to understand which signaling pathways are important for immunosuppressive properties in B cells. In vitro analysis of IL-35 reporter B cells revealed a synergy between the BCR and TLR4 signaling pathways is sufficient to induce IL-35 expression. However, in vivo, B cell receptor activation, as opposed to MyD88 signaling in B cells, is central to B cell-mediated suppression and promotion of pancreatic cancer growth. Further analysis identified protein kinase D2 (PKD2) as being a key downstream regulator of IL-35 expression in B cells. Regulatory B cells with an inactivating mutation in PKD2 failed to produce IL-35 or fully suppress effector T cell function in vitro. Furthermore, inhibition of PKD in B cells decreased tumor growth and promoted effector T cell function upon adoptive transfer into B cell-deficient mice. Collectively, these data provide insight into how regulatory B cell function is promoted in pancreatic cancer and identify potential therapeutic targets to restrain this function.
Revisiting the Development of Vaccines Against Pathogenic Leptospira: Innovative Approaches, Present Challenges, and Future Perspectives
Authors: Barazzone Giovana C. , Teixeira Aline F. , Azevedo Bruna O. P. , Damiano Deborah K. , Oliveira Marcos P. , Nascimento Ana L. T. O. , Lopes Alexandre P. Y.
Published: January 2022

Human vaccination against leptospirosis has been relatively unsuccessful in clinical applications despite an expressive amount of vaccine candidates has been tested over years of research. Pathogenic Leptospira encompass a great number of serovars, most of which do not cross-react, and there has been a lack of genetic tools for many years. These obstacles have hampered the understanding of the bacteria’s biology and, consequently, the identification of an effective antigen. Thus far, many approaches have been used in an attempt to find a cost-effective and broad-spectrum protective antigen(s) against the disease. In this extensive review, we discuss several strategies that have been used to develop an effective vaccine against leptospirosis, starting with Leptospira-inactivated bacterin, proteins identified in the genome sequences of pathogenic Leptospira, including reverse vaccinology, plasmid DNA, live vaccines, chimeric multi-epitope, and toll- and nod-like receptors agonists. This overview should be able to guide scientists working in the field to select potential antigens and to choose the appropriate formulation to administer the candidates.