bluuebunny/crossref_metadata_2025 huggingface dataset:
Acid Corrosion Analysis in the Initial Condensation Zone of a H2O/CO2 Turbine
Authors: Ma Ziyue , Wang Xiaofang , Yang Jinguang , Wang Wei , Shao Wenyang , Jiang Xiaowu
Published: June 2021

A supercritical H2O/CO2 turbine is a key piece of equipment for the coal gasification in the supercritical water (CGSW) cycle to achieve conversion of heat into power. Compared with a traditional steam turbine, the working medium of an H2O/CO2 turbine has a relatively high CO2 concentration. In the initial condensation zone (ICZ), steam condenses into droplets on the turbine blades and the droplets combine with CO2 to form carbonic acid, which corrodes the turbine blades. In order to research the characteristics of acid corrosion in the ICZ of a H2O/CO2 turbine, the acid corrosion rate of the blades in the ICZ of the H2O/CO2 turbine was calculated and analyzed based on the three-dimensional CFD (3D CFD) method and a one-dimensional numerical model of CO2 corrosion. The results suggest that acid corrosion rates decrease stage by stage in the ICZ due to the reduction in temperature and pressure. Rotor blades in the first stage in the ICZ suffer the worst and form a corrosion zone at the trailing edge of the blade and on the pressure surface. The decline of efficiency caused by corrosion settles down to a relatively steady value of 0.6% for a 10 year service time. Moreover, the corrosion area for the last two stages shrinks with the service time due to the rearward movement of the ICZ.
Two-Phase Heuristic Algorithm for Integrated Airline Fleet Assignment and Routing Problem
Authors: Özkır Vildan , Özgür Mahmud Sami
Published: June 2021

High profitability and high costs have stiffened competition in the airline industry. The main purpose of the study is to propose a computationally efficient algorithm for integrated fleet assignments and aircraft routing problems for a real-case hub and spoke airline planning problem. The economic concerns of airline operations have led to the need for minimising costs and increasing the ability to meet rising demands. Since fleets are the most limited and valuable assets of airline carriers, the allocation of aircraft to scheduled flights directly affects profitability/market share. The airline fleet assignment problem (AFAP) addresses the assignment of aircraft, each with a different capacity, capability, availability, and requirement, to a given flight schedule. This study proposes a mathematical model and heuristic method for solving a real-life airline fleet assignment and aircraft routing problem. We generate a set of problem instances based on real data and conduct a computational experiment to assess the performance of the proposed algorithm. The numerical study and experimental results indicate that the heuristic algorithm provides optimal solutions for the integrated fleet assignment and aircraft routing problem. Furthermore, a computational study reveals that compared with the heuristic method, solving the mathematical model takes significantly longer to execute.
Analysis of Restructuring the Mexican Electricity Sector to Operate in a Wholesale Energy Market
Authors: Percino-Picazo Juan C. , Llamas-Terres Armando R. , Viramontes-Brown Federico A.
Published: June 2021

This paper analyzes the energy reform that has taken place in Mexico since 2013, driven by steady growth in energy demand and insufficient economic resources. The relevant points in the restructuring process are discussed, shedding light on the impact of recent governmental actions not aligned with the original spirit of the law. This research uses a framework and fundamentals of a well-organized structural process called the textbook model, making a comparative analysis of Mexican reform. It proceeds by presenting the Mexican Electrical System in numbers and how it is affected by the present government’s restructuring process providing positive and negative impacts of several implementations. The main objectives of restructuring were carried out to attract private investment and increase the reliability and efficiency of the system. During the first four years, the reform has attracted investment, in diminishing form in generation but not in transmission and distribution. Therefore, the main reason for this is explained and a brief analysis and the roots of these failures are presented. It is shown that recent political decisions tend to keep the system operator and regulatory agencies under Federal Government control so that it is acting against the main objectives of the original reform. Finally, a summary of the deviation of the reality from the existing law is outlined.
Design and Large Temperature Jump Testing of a Modular Finned-Tube Carbon–Ammonia Adsorption Generator for Gas-Fired Heat Pumps
Authors: Metcalf Steven , Rivero-Pacho Ángeles , Critoph Robert
Published: June 2021

Gas-fired heat pumps are a potential replacement for condensing boilers, utilizing fossil-fuel resources more efficiently and reducing the amount of biogas or hydrogen required in sustainable gas grids. However, their adoption has been limited due to their large size and high capital cost, resulting in long payback times. For adsorption-based heat pumps, the major development challenge is to maximize the rate of heat transfer to the adsorbent, whilst minimizing the thermal mass. This work develops a modular finned-tube carbon–ammonia adsorption generator that incorporates the adsorbent in highly compacted 3-mm layers between aluminum fins. Manufacturing techniques that are amenable to low cost and high-volume production were developed. The module was tested using the large temperature jump (LTJ) method and achieved a time constant for adsorption and desorption of 50 s. The computational model predicted that if incorporated into two adsorption generators of 6 L volume each, they could be used to construct a gas-fired heat pump with a 10 kW heat output and a gas utilization efficiency (GUE, the ratio of useful heat output to higher calorific value of gas used) of 1.2.
Fourth-Order Comprehensive Adjoint Sensitivity Analysis (4th-CASAM) of Response-Coupled Linear Forward/Adjoint Systems: I. Theoretical Framework
Authors: Cacuci Dan Gabriel
Published: June 2021

The most general quantities of interest (called “responses”) produced by the computational model of a linear physical system can depend on both the forward and adjoint state functions that describe the respective system. This work presents the Fourth-Order Comprehensive Adjoint Sensitivity Analysis Methodology (4th-CASAM) for linear systems, which enables the efficient computation of the exact expressions of the 1st-, 2nd-, 3rd- and 4th-order sensitivities of a generic system response, which can depend on both the forward and adjoint state functions, with respect to all of the parameters underlying the respective forward/adjoint systems. Among the best known such system responses are various Lagrangians, including the Schwinger and Roussopoulos functionals, for analyzing ratios of reaction rates, the Rayleigh quotient for analyzing eigenvalues and/or separation constants, etc., which require the simultaneous consideration of both the forward and adjoint systems when computing them and/or their sensitivities (i.e., functional derivatives) with respect to the model parameters. Evidently, such responses encompass, as particular cases, responses that may depend just on the forward or just on the adjoint state functions pertaining to the linear system under consideration. This work also compares the CPU-times needed by the 4th-CASAM versus other deterministic methods (e.g., finite-difference schemes) for computing response sensitivities These comparisons underscore the fact that the 4th-CASAM is the only practically implementable methodology for obtaining and subsequently computing the exact expressions (i.e., free of methodologically-introduced approximations) of the 1st-, 2nd, 3rd- and 4th-order sensitivities (i.e., functional derivatives) of responses to system parameters, for coupled forward/adjoint linear systems. By enabling the practical computation of any and all of the 1st-, 2nd, 3rd- and 4th-order response sensitivities to model parameters, the 4th-CASAM makes it possible to compare the relative values of the sensitivities of various order, in order to assess which sensitivities are important and which may actually be neglected, thus enabling future investigations of the convergence of the (multivariate) Taylor series expansion of the response in terms of parameter variations, as well as investigating the range of validity of other important quantities (e.g., response variances/covariance, skewness, kurtosis, etc.) that are derived from Taylor-expansion of the response as a function of the model’s parameters. The 4th-CASAM presented in this work provides the basis for significant future advances towards overcoming the “curse of dimensionality” in sensitivity analysis, uncertainty quantification and predictive modeling.
Use of Acidithiobacillus thiooxidans and Acidithiobacillus ferrooxidans in the Recovery of Heavy Metals from Landfill Leachates
Authors: Kamizela Tomasz , Grobelak Anna , Worwag Malgorzata
Published: June 2021

Among the methods used to remove metals and their compounds from landfill leachates with low application costs and high efficiency are bioleaching and biosorption. The most effective bacteria used in the metal removal process are Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans. The aim of the study was to determine the usefulness of the A. ferrooxidans and A. thiooxidans population in removing heavy metals from landfill leachate. In addition, development opportunities for bacterial population using landfill leachate as growth medium were identified. The substrate for the research was the raw leachate before the reverse osmosis process. In order to increase the efficiency of trace elements removal and recovery from leachate, variable combinations have been used which differ by the addition of sulfuric acid, A. ferrooxidans culture, A. thiooxidans culture, mixed culture containing populations of both bacteria, and elemental sulfur. Based on the research, it was found that the removal of heavy metals from leachate was a selective process. High bioleaching efficiency, from 80% to 90%, was obtained for all metals for which the sample acidification or sulfur addition was used. The simultaneous combination of both these additives turned out to be the most advantageous. The A. thiooxidans culture was the most effective in bioleaching reverse osmosis effluents. For the A. ferrooxidans culture used, much lower efficiencies were obtained, while by contrast, the use of mixed culture of two bacterium species had no significant effect.
A Sequential Optimization-Simulation Approach for Planning the Transition to the Low Carbon Freight System with Case Study in the North Island of New Zealand
Authors: Gallardo Patricio , Murray Rua , Krumdieck Susan
Published: June 2021

Freight movement has always been, and always will be an essential activity. Freight transport is one of the most challenging sectors to transition to net-zero carbon. Traffic assignment, mode allocation, network planning, hub location, train scheduling and terminal design problem-solving have previously been used to address cost and operation efficiencies. In this study, the interdisciplinary transition innovation, management and engineering (InTIME) methodology was used for the conceptualization, redesign and redevelopment of the existing freight systems to achieve a downshift in fossil energy consumption. The fourth step of the InTIME methodology is the conceptualization of a long-term future intermodal transport system that can serve the current freight task. The novelty of our approach stands in considering the full range of freight supply chain factors as a whole, using an optimization-simulation approach as if we were designing the low-carbon system of 2121. For the optimization, ArcGIS software was used to set up a multimodal network model. Route and mode selection were delivered through the optimization of energy use within the network. Complementarily, Anylogic software was used to build a GIS-based discrete event simulation model and set up different experiments to enhance the solution offered by the network analysis. The results outline the resources needed (i.e., number of railway tracks, train speed, size of railyards, number of cranes and forklifts at terminals) to serve the freight task. The results can be backcast to reveal the most efficient investments in the near term. In the case of New Zealand’s North Island, the implementation of strategic terminals, with corresponding handling resources and railyards, could deliver 47% emissions reduction from the sector by 2030, ahead of longer lead-time upgrades like electrification of the railway infrastructure.
CFD Simulations for Performance Enhancement of a Solar Chimney Power Plant (SCPP) and Techno-Economic Feasibility for a 5 MW SCPP in an Indian Context
Authors: Ganguli Arijit , Deshpande Sagar , Pandit Aniruddha
Published: June 2021

The use of solar energy for power generation using the innovative solar chimney concept has been explored by many researchers mostly with the help of analytical models as well as CFD simulations while experimental studies for a pilot and bench scale facilities have been carried out. The efficiencies of these chimneys, however, are less than 1% (~0.07% for 50 kW pilot plant similar to Manzanares plant in Spain). In the present study, an effort has been made to make modifications in the chimney design to improve the efficiency of the chimney in terms of power generation. CFD simulations have been carried out for this modified design and the efficiency is seen to improve to 0.12% for a 50 kW chimney. Furthermore, a techno-economic feasibility analysis has been carried out for a conventional 5 MW solar power plant which can be installed on the western part of India, which receives good solar irradiation throughout the year. Two cases with and without government subsidies have been considered. It is observed that a high rate of return (~20.4%) is obtained for a selling price of electricity of Rs 5 per kWh with government subsidy, while a rate of return of ~19% is obtained for Rs 10 per kWh without government subsidy.
Customized yet Standardized Temperature Derivatives: A Non-Parametric Approach with Suitable Basis Selection for Ensuring Robustness
Authors: Matsumoto Takuji , Yamada Yuji
Published: June 2021

Previous studies have demonstrated that non-parametric hedging models using temperature derivatives are highly effective in hedging profit/loss fluctuation risks for electric utilities. Aiming for the practical applications of these methods, this study performs extensive empirical analyses and makes methodological customizations. First, we consider three types of electric utilities being exposed to risks of “demand”, “price”, and their “product (multiplication)”, and examine the design of an appropriate derivative for each utility. Our empirical results show that non-parametrically priced derivatives can maximize the hedge effect when a hedger bears a “price risk” with high nonlinearity to temperature. In contrast, standard derivatives are more useful for utilities with only “demand risk” in having a comparable hedge effect and in being liquidly traded. In addition, the squared prediction error derivative on temperature has a significant hedge effect on both price and product risks as well as a certain effect on demand risk, which illustrates its potential as a new standard derivative. Furthermore, spline basis selection, which may be overlooked by modeling practitioners, improves hedge effects significantly, especially when the model has strong nonlinearities. Surprisingly, the hedge effect of temperature derivatives in previous studies is improved by 13–53% by using an appropriate new basis.
An Experimental Study of a Wine Batch Distillation in a Copper Pot Still Heated by Gas
Authors: Rougier Marie , Bellettre Jérôme , Luo Lingai
Published: June 2021

Wine batch distillation in a copper pot still heated directly by combustion is unusual. It is a niche sector. Few studies are available on the subject and even fewer have studied it from a thermal point of view. In a context of ecology awareness and regularly increasing gas taxes, finding less energy consuming and more performing solutions has become necessary. Two methods already exist to recover energy for such pot stills, however improvements could be made. The present study focuses on the heating zone of an experimental pot still. First, a thermal diagnosis based on a nodal model is conducted in transient regime. The major sites of energy expenditure and waste are thus identified and quantified. Results highlight significant losses both in combustion smoke and by conduction to the environment resulting from a limited thermal performance of the system. Proposals are then made to enhance it. For instance, the preheating could be optimized and more thermal waste could be recovered.
Identification of Independent Variables to Assess Green-Building Development in China Based on Grounded Theory
Authors: Zhang Ying , Kang Jian , Jin Hong
Published: June 2021

Background: Development of green building as future buildings has become a trend and played a significant role in changing the general direction of building development and creating an environment for sustainable development ’People-centric’ explores the relationship between people and building development. From the perspective of users, what are the influencing factors of green building? What is the relationship between independent variables? The authors link this issue to the development of green building and gaining a clearer understanding and direction. Methods: The authors applied grounded theory and intensity sampling to analyse the relationships of independent variables. Results: The findings of this study reveal the four core factors affecting how independent variables get to learn about green building, which are ‘personal perception elements’, ‘social elements’, ‘organisational elements’, and ‘architectural properties’. Conclusions: The authors also analysed the relationships between the independent variables to explore construction theory for helping green building better respond to people’s demand and pushing forward its development. In this case, the ’people-centric’ green building further improves the urban living environment.
Model of Flow Resistance Coefficient for a Fragment of a Porous Material Deposit with Skeletal Structure
Authors: Wałowski Grzegorz
Published: June 2021

The hydrodynamic conditions resulting from the permeability of porous materials are based not only on the assessment of the gas flow through these materials, but also the losses related to the pressure energy in this flow. Flow resistance is a direct measure of this loss. The aim of this experimental research was to evaluate the flow resistance of the porous material in relation to the gas flow. The research was carried out on a material with a slit-porous structure. The tests were carried out on a system for measuring gas permeability under the conditions of gas bubbling through the char. The issue of the total pressure drop process in the porous bed was considered in the Reynolds number category. The coefficient of flow resistance for the char was determined and the value of this coefficient was compared with the gas stream, and an experimental evaluation of the total pressure drop on the porous bed was made. The novelty of this article is the determination of the tortuosity and the gas permeability coefficient for a solid of any shape—a rigid skeleton.
Regional Diversity of Technical Efficiency in Agriculture as a Results of an Overinvestment: A Case Study from Poland
Authors: Pawłowski Krzysztof Piotr , Czubak Wawrzyniec , Zmyślona Jagoda
Published: June 2021

Ensuring adequate profitability of production, which can be ensured by optimal investments, can encourage farmers to be more caring about sustainable development. Several existing studies indicate that technical efficiency in agriculture varies regionally. Investments comprise a basic way to increase efficiency and thus reduce polarisation between regions. However, contrary to established assumptions, not every investment leads to increased efficiency, which entails a phenomenon of overinvestment. Investments should, by definition, be positively correlated with efficiency. However, existing studies indicate the existence of a significant problem of overinvestment, where increased efficiency may not occur. While for about 40% of farms in Poland the scale of investments can be assessed as optimal, more than quarter of farms exhibited absolute overinvestment and nearly one in five farms is underinvested. In response to this problem, this study aimed to identify regional differences in Poland with regard to overinvestment in farms, as well as to determine changes in farm efficiency depending on the region and level of overinvestment. The source material used in the following article consisted of unpublished Farm Accountancy Data Network (FADN) microdata derived from the DG AGRI of the European Commission. The study covered the period 2004–2015. For an original classification of farms according to their level of overinvestment the technical efficiency, using the stochastic frontier analysis approach, was used for determining regional differences that occurred as a result of overinvestment. Stochastic frontier analysis shown noticeable differences in the average technical efficiency for different overinvestment groups. As expected, underinvested farms are the least efficient (general in Poland and in all analyzed regions) and average technical efficiency did not increase. Interestingly, optimally investing farms do not have the highest technical efficiency. Higher efficiency was achieved by both relatively and absolutely overinvested farms. This is due to the fact that in order to produce efficiently in agriculture, it is necessary to at least maintain the level of tangible assets provision, and preferably to increase it as well. In terms of overinvestment levels, farm structure does not differ significantly between individual regions in Poland. However, there are differences between regions in terms of farm efficiency within each group. In all regions, only the underinvested farms did not increase their efficiency over the period under review and the highest efficiency growth rate was in regions where farms were least efficient at baseline.
Comparative Study on the Calendar Aging Behavior of Six Different Lithium-Ion Cell Chemistries in Terms of Parameter Variation
Authors: Geisbauer Christian , Wöhrl Katharina , Koch Daniel , Wilhelm Gudrun , Schneider Gerhard , Schweiger Hans-Georg
Published: June 2021

The degradation of lithium-ion cells is an important aspect, not only for quality management, but also for the customer of the application like, e.g., scooters or electric vehicles. During the lifetime of the system, the overall health on the battery plays a key role in its depreciation. Therefore, it is necessary to monitor the health of the battery during operation, i.e., cycle life, but also during stationary conditions, i.e., calendar aging. In this work, the degradation due to calendar aging is analyzed for six different cell chemistries in terms of capacity degradation and impedance increase and their performance are being compared. In a new proposed metric, the relative deviations between various cells with the exact identical aging history are being analyzed for their degradation effects and their differences, which stands out in comparison to similar research. The capacity loss was found to be most drastic at 60 °C and at higher storage voltages, even for titanate-oxide cells. LiNiMnCoO2 (NMC), LiNiCoAlO2 (NCA) and Li2TiO3 (LTO) cells at 60 °C showed the most drastic capacity decrease. NMC and NCA cells at 60 °C and highest storage voltage did not show any open circuit voltage, as their current interrupt mechanism triggered. The effect of aging shows no uniform impact on the changes in the capacity variance when comparing different aging conditions, with respect to the evaluated standard deviation for all cells. The focus of this work was on the calendar aging effect and may be supplemented in a second study for cyclic aging.
Modeling, Analysis and Optimization of Grid-Integrated and Islanded Solar PV Systems for the Ethiopian Residential Sector: Considering an Emerging Utility Tariff Plan for 2021 and Beyond
Authors: Mekonnen Tefera , Bhandari Ramchandra , Ramayya Venkata
Published: June 2021

Currently, difficulties such as the depletion of fossil fuel resources and the associated environmental pollution have driven the rise of other energy systems based on green energy sources. In this research, modeling and a viability study of grid-connected and islanded photovoltaic (PV) power systems for supplying the residential load in Mekelle City, Ethiopia, were carried out considering the country’s emerging utility tariff plan for 2021 and beyond. The technical viability of the proposed supply option was analyzed using PVGIS, PVWatts and HOMER Pro tool, while the economic and environmental optimization aspects were carried out using HOMER Pro. Sensitivity analyses and output comparisons among the three renewable energy simulation tools are presented. The results showed that under the consideration of an incremental electricity tariff plan (up to 2021), the analyzed cost of energy of the grid/PV system is around 12% lower than the utility grid tariff. Moreover, we also found that by taking the continuous global solar PV cost reduction into account, the cost of energy of the modeled islanded operation of solar PV power units totally broke the grid tariff in Ethiopia after 2029 based on the tariff for 2021 and well before with the expected escalation of the grid tariff on an annual basis. The technical performance of the system realized through PVGIS and PVWatts was almost comparable to the HOMER Pro outputs. Thus, this investigation will offer a clear direction to the concerned target groups and policy developers in the evolution of PV power supply options throughout the technically viable locations in the country.
Influence of Riverbed Incision and Hydrological Evolution on Water Quality and Water Age Based on Numerical Simulation: A Case Study of the Minjiang Estuary
Authors: Zhang Peng , Li Lanyimin , Wang Yishu , Shi Chengchun , Fan Chenchen
Published: June 2021

In recent years, problems such as water quality deterioration, saltwater invasion, and low oxygen have appeared in estuaries all over the world. The Minjiang River in Fujian, as a typical tidal estuary area, is facing these thorny problems. In this paper, the effects of topography and hydrologic evolution on the water age and water quality of the lower reaches of the Minjiang River were simulated by building a hydrodynamic and water quality model. The results show that: (1) It was found that the riverbed incision of the lower reaches of the Minjiang River led to the overall decline of river water level, the increase of river volume, and the increase of downstream water age, which eventually led to the decrease of dissolved oxygen (DO) and the deterioration of water quality in the downstream from Shuikou to Baiyantan. However, the decline of topography led to the increase of tidal volume in the estuary, the enhancement of the dilution effect of oxygen-rich water bodies in the open sea, and the increase of DO in the lower reaches of Baiyantan. (2) Under no tidal action, the concentration of pollutants in the water of the North Channel increased, the DO decreased, and the DO decreased from Baiyantan to the offshore water. After the enhancement of tidal action, the dilution of oxygen-enriched water from the offshore water increased, and the DO increased. (3) The hydrological and water quality characteristics of the upper part of the lower reaches of the Minjiang River were mainly controlled by topography, runoff, and pollutant discharge, which were more affected by the tidal current transport operation and pollutant discharge near the open sea. In recent decades, the deterioration of water quality and the aggravation of saltwater intrusion in the Minjiang River were closely related to the serious topographic downcutting. The results provide a scientific basis for revealing the deterioration of estuary water quality and long-term management of the estuary.
Large Animal Models for Investigating Cell Therapies of Stress Urinary Incontinence
Authors: Amend Bastian , Harland Niklas , Knoll Jasmin , Stenzl Arnulf , Aicher Wilhelm K.
Published: June 2021

Stress urinary incontinence (SUI) is a significant health concern for patients affected, impacting their quality of life severely. To investigate mechanisms contributing to SUI different animal models were developed. Incontinence was induced under defined conditions to explore the pathomechanisms involved, spontaneous recovery, or efficacy of therapies over time. The animal models were coined to mimic known SUI risk factors such as childbirth or surgical injury. However, animal models neither reflect the human situation completely nor the multiple mechanisms that ultimately contribute to the pathogenesis of SUI. In the past, most SUI animal studies took advantage of rodents or rabbits. Recent models present for instance transgenic rats developing severe obesity, to investigate metabolic interrelations between the disorder and incontinence. Using recombinant gene technologies, such as transgenic, gene knock-out or CRISPR-Cas animals may narrow the gap between the model and the clinical situation of patients. However, to investigate surgical regimens or cell therapies to improve or even cure SUI, large animal models such as pig, goat, dog and others provide several advantages. Among them, standard surgical instruments can be employed for minimally invasive transurethral diagnoses and therapies. We, therefore, focus in this review on large animal models of SUI.
Dietary Nanoparticles Interact with Gluten Peptides and Alter the Intestinal Homeostasis Increasing the Risk of Celiac Disease
Authors: Mancuso Clara , Re Francesca , Rivolta Ilaria , Elli Luca , Gnodi Elisa , Beaulieu Jean-François , Barisani Donatella
Published: June 2021

The introduction of metallic nanoparticles (mNPs) into the diet is a matter of concern for human health. In particular, their effect on the gastrointestinal tract may potentially lead to the increased passage of gluten peptides and the activation of the immune response. In consequence, dietary mNPs could play a role in the increasing worldwide celiac disease (CeD) incidence. We evaluated the potential synergistic effects that peptic-tryptic-digested gliadin (PT) and the most-used food mNPs may induce on the intestinal mucosa. PT interaction with mNPs and their consequent aggregation was detected by transmission electron microscopy (TEM) analyses and UV–Vis spectra. In vitro experiments on Caco-2 cells proved the synergistic cytotoxic effect of PT and mNPs, as well as alterations in the monolayer integrity and tight junction proteins. Exposure of duodenal biopsies to gliadin plus mNPs triggered cytokine production, but only in CeD biopsies. These results suggest that mNPs used in the food sector may alter intestinal homeostasis, thus representing an additional environmental risk factor for the development of CeD.
microRNAs in the Regulation of Melanogenesis
Authors: Hushcha Yekatsiaryna , Blo Irene , Oton-Gonzalez Lucia , Mauro Giulia Di , Martini Fernanda , Tognon Mauro , Mattei Monica De
Published: June 2021

Melanogenesis is the process leading to the synthesis of melanin, the main substance that influences skin color and plays a pivotal role against UV damage. Altered melanogenesis is observed in several pigmentation disorders. Melanogenesis occurs in specialized cells called melanocytes, physically and functionally related by means of autocrine and paracrine interplay to other skin cell types. Several external and internal factors control melanin biosynthesis and operate through different intracellular signaling pathways, which finally leads to the regulation of microphthalmia-associated transcription factor (MITF), the key transcription factor involved in melanogenesis and the expression of the main melanogenic enzymes, including TYR, TYRP-1, and TYRP-2. Epigenetic factors, including microRNAs (miRNAs), are involved in melanogenesis regulation. miRNAs are small, single-stranded, non-coding RNAs, of approximately 22 nucleotides in length, which control cell behavior by regulating gene expression, mainly by binding the 3′ untranslated region (3′-UTR) of target mRNAs. This review collects data on the miRNAs involved in melanogenesis and how these miRNAs can modulate target gene expression. Bringing to light the biological function of miRNAs could lead to a wider understanding of epigenetic melanogenesis regulation and its dysregulation. This knowledge may constitute the basis for developing innovative treatment approaches for pigmentation dysregulation.
The Mechanism of Drug Nephrotoxicity and the Methods for Preventing Kidney Damage
Authors: Kwiatkowska Ewa , Domański Leszek , Dziedziejko Violetta , Kajdy Anna , Stefańska Katarzyna , Kwiatkowski Sebastian
Published: June 2021

Acute kidney injury (AKI) is a global health challenge of vast proportions, as approx. 13.3% of people worldwide are affected annually. The pathophysiology of AKI is very complex, but its main causes are sepsis, ischemia, and nephrotoxicity. Nephrotoxicity is mainly associated with the use of drugs. Drug-induced AKI accounts for 19–26% of all hospitalized cases. Drug-induced nephrotoxicity develops according to one of the three mechanisms: (1) proximal tubular injury and acute tubular necrosis (ATN) (a dose-dependent mechanism), where the cause is related to apical contact with drugs or their metabolites, the transport of drugs and their metabolites from the apical surface, and the secretion of drugs from the basolateral surface into the tubular lumen; (2) tubular obstruction by crystals or casts containing drugs and their metabolites (a dose-dependent mechanism); (3) interstitial nephritis induced by drugs and their metabolites (a dose-independent mechanism). In this article, the mechanisms of the individual types of injury will be described. Specific groups of drugs will be linked to specific injuries. Additionally, the risk factors for the development of AKI and the methods for preventing and/or treating the condition will be discussed.
Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (Solanum tuberosum L.)
Authors: Schumacher Christina , Thümecke Susanne , Schilling Florian , Köhl Karin , Kopka Joachim , Sprenger Heike , Hincha Dirk Karl , Walther Dirk , Seddig Sylvia , Peters Rolf , Zuther Ellen , Haas Manuela , Horn Renate
Published: June 2021

Drought represents a major abiotic stress factor negatively affecting growth, yield and tuber quality of potatoes. Quantitative trait locus (QTL) analyses were performed in cultivated potatoes for drought tolerance index DRYM (deviation of relative starch yield from the experimental median), tuber starch content, tuber starch yield, tuber fresh weight, selected transcripts and metabolites under control and drought stress conditions. Eight genomic regions of major interest for drought tolerance were identified, three representing standalone DRYM QTL. Candidate genes, e.g., from signaling pathways for ethylene, abscisic acid and brassinosteroids, and genes encoding cell wall remodeling enzymes were identified within DRYM QTL. Co-localizations of DRYM QTL and QTL for tuber starch content, tuber starch yield and tuber fresh weight with underlying genes of the carbohydrate metabolism were observed. Overlaps of DRYM QTL with metabolite QTL for ribitol or galactinol may indicate trade-offs between starch and compatible solute biosynthesis. Expression QTL confirmed the drought stress relevance of selected transcripts by overlaps with DRYM QTL. Bulked segregant analyses combined with next-generation sequencing (BSAseq) were used to identify mutations in genes under the DRYM QTL on linkage group 3. Future analyses of identified genes for drought tolerance will give a better insight into drought tolerance in potatoes.
Multiple Functions of MYB Transcription Factors in Abiotic Stress Responses
Authors: Wang Xiaopei , Niu Yanli , Zheng Yuan
Published: June 2021

Plants face a more volatile environment than other organisms because of their immobility, and they have developed highly efficient mechanisms to adapt to stress conditions. Transcription factors, as an important part of the adaptation process, are activated by different signals and are responsible for the expression of stress-responsive genes. MYB transcription factors, as one of the most widespread transcription factor families in plants, participate in plant development and responses to stresses by combining with MYB cis-elements in promoters of target genes. MYB transcription factors have been extensively studied and have proven to be critical in the biosynthesis of secondary metabolites in plants, including anthocyanins, flavonols, and lignin. Multiple studies have now shown that MYB proteins play diverse roles in the responses to abiotic stresses, such as drought, salt, and cold stresses. However, the regulatory mechanism of MYB proteins in abiotic stresses is still not well understood. In this review, we will focus mainly on the function of Arabidopsis MYB transcription factors in abiotic stresses, especially how MYB proteins participate in these stress responses. We also pay attention to how the MYB proteins are regulated in these processes at both the transcript and protein levels.
Overexpression of miR-124 in Motor Neurons Plays a Key Role in ALS Pathological Processes
Authors: Vaz Ana Rita , Vizinha Daniela , Morais Hermes , Colaço Ana Rita , Loch-Neckel Gecioni , Barbosa Marta , Brites Dora
Published: June 2021

miRNA(miR)-124 is an important regulator of neurogenesis, but its upregulation in SOD1G93A motor neurons (mSOD1 MNs) was shown to associate with neurodegeneration and microglia activation. We used pre-miR-124 in wild-type (WT) MNs and anti-miR-124 in mSOD1 MNs to characterize the miR-124 pathological role. miR-124 overexpression in WT MNs produced a miRNA profile like that of mSOD1 MNs (high miR-125b; low miR-146a and miR-21), and similarly led to early apoptosis. Alterations in mSOD1 MNs were abrogated with anti-miR-124 and changes in their miRNAs mostly recapitulated by their secretome. Normalization of miR-124 levels in mSOD1 MNs prevented the dysregulation of neurite network, mitochondria dynamics, axonal transport, and synaptic signaling. Same alterations were observed in WT MNs after pre-miR-124 transfection. Secretome from mSOD1 MNs triggered spinal microglia activation, which was unno-ticed with that from anti-miR-124-modulated cells. Secretome from such modulated MNs, when added to SC organotypic cultures from mSOD1 mice in the early symptomatic stage, also coun-teracted the pathology associated to GFAP decrease, PSD-95 and CX3CL1-CX3CR1 signaling im-pairment, neuro-immune homeostatic imbalance, and enhanced miR-124 expression levels. Data suggest that miR-124 is implicated in MN degeneration and paracrine-mediated pathogenicity. We propose miR-124 as a new therapeutic target and a promising ALS biomarker in patient sub-populations.
Flavonone 3-hydroxylase Relieves Bacterial Leaf Blight Stress in Rice via Overaccumulation of Antioxidant Flavonoids and Induction of Defense Genes and Hormones
Authors: Jan Rahmatullah , Aaqil Khan Muhammad , Asaf Sajjad , Lubna  , Park Jae-Ryoung , Lee In-Jung , Kim Kyung-Min
Published: June 2021

Efficient accumulation of flavonoids is important for increased tolerance to biotic stress. Although several plant defense mechanisms are known, the roles of many pathways, proteins, and secondary metabolites in stress tolerance are unknown. We generated a flavanone 3-hydroxylase (F3H) overexpressor rice line and inoculated Xanthomonas Oryzae pv. oryzae and compared the control and wildtype inoculated plants. In addition to promoting plant growth and developmental maintenance, the overexpression of F3H increased the accumulation of flavonoids and increased tolerance to bacterial leaf blight (BLB) stress. Moreover, leaf lesion length was higher in the infected wildtype plants compared with infected transgenics. Kaempferol and quercetin, which scavenge reactive oxygen species, overaccumulated in transgenic lines compared with wildtypes in response to pathogenic infection, detected by scanning electron microscopy and spectrophotometry. The induction of F3H altered the antioxidant system and reduced the levels of glutathione peroxidase activity and malondialdehyde (MDA) contents in the transgenic lines compared with the wildtypes. Downstream gene regulation analysis showed that the expression of F3H increased the regulation of flavonol synthase (FLS), dihydroflavonol 4-reductase (DFR), and slender rice mutant (SLR1) during BLB stress. The analysis of SA and JA signaling revealed an antagonistic interaction between both hormones and that F3H induction significantly promoted SA and inhibited JA accumulation in the transgenic lines. SA-dependent nonexpressor pathogenesis-related (NPR1) and Xa1 showed significant upregulation in the infected transgenic lines compared with the infected control and wildtype lines. Thus, the overexpression of F3H was essential for increasing BLB stress tolerance.
Activity of CdTe Quantum-Dot-Tagged Superoxide Dismutase and Its Analysis in Capillary Electrophoresis
Authors: Zaręba Natalia , Lewandowski Łukasz , Kunachowicz Dominika , Kizek Rene , Kepinska Marta
Published: June 2021

Quantum dots (QDs) have a broad range of applications in cell biolabeling, cancer treatment, metastasis imaging, and therapeutic drug monitoring. Despite their wide use, relatively little is known about their influence on other molecules. Interactions between QDs and proteins can influence the properties of both nanoparticles and proteins. The effect of mercaptosuccinic acid-capped CdTe QDs on intercellular copper–zinc superoxide dismutase (SOD1)—one of the main enzymatic antioxidants—was investigated. Incubation of SOD1 with QDs caused an increase in SOD1 activity, unlike in the case of CdCl2, which inhibited SOD1. Moreover, this effect on SOD1 increased with the size and potential of QDs, although the effect became clearly visible in higher concentrations of QDs. The intensity of QD-SOD1 fluorescence, analyzed with the use of capillary electrophoresis with laser-induced fluorescence detection, was dependent on SOD1 concentration. In the case of green QDs, the fluorescence signal decreased with increasing SOD1 concentration. In contrast, the signal strength for Y-QD complexes was not dependent on SOD1 dilutions. The migration time of QDs and their complexes with SOD1 varied depending on the type of QD used. The migration time of G-QD complexes with SOD1 differed slightly. However, in the case of Y-QD complexes with SOD1, the differences in the migration time were not dependent on SOD concentration. This research shows that QDs interact with SOD1 and the influence of QDs on SOD activity is size-dependent. With this knowledge, one might be able to control the activation/inhibition of specific enzymes, such as SOD1.
Roles of Key Ion Channels and Transport Proteins in Age-Related Hearing Loss
Authors: Bazard Parveen , Frisina Robert D. , Acosta Alejandro A. , Dasgupta Sneha , Bauer Mark A. , Zhu Xiaoxia , Ding Bo
Published: June 2021

The auditory system is a fascinating sensory organ that overall, converts sound signals to electrical signals of the nervous system. Initially, sound energy is converted to mechanical energy via amplification processes in the middle ear, followed by transduction of mechanical movements of the oval window into electrochemical signals in the cochlear hair cells, and finally, neural signals travel to the central auditory system, via the auditory division of the 8th cranial nerve. The majority of people above 60 years have some form of age-related hearing loss, also known as presbycusis. However, the biological mechanisms of presbycusis are complex and not yet fully delineated. In the present article, we highlight ion channels and transport proteins, which are integral for the proper functioning of the auditory system, facilitating the diffusion of various ions across auditory structures for signal transduction and processing. Like most other physiological systems, hearing abilities decline with age, hence, it is imperative to fully understand inner ear aging changes, so ion channel functions should be further investigated in the aging cochlea. In this review article, we discuss key various ion channels in the auditory system and how their functions change with age. Understanding the roles of ion channels in auditory processing could enhance the development of potential biotherapies for age-related hearing loss.
Virus–Host Interaction Gets Curiouser and Curiouser. PART II: Functional Transcriptomics of the E. coli DksA-Deficient Cell upon Phage P1vir Infection
Authors: Cech Grzegorz M. , Szalewska-Pałasz Agnieszka , Potrykus Katarzyna , Kloska Anna
Published: June 2021

The virus–host interaction requires a complex interplay between the phage strategy of reprogramming the host machinery to produce and release progeny virions, and the host defense against infection. Using RNA sequencing, we investigated the phage–host interaction to resolve the phenomenon of improved lytic development of P1vir phage in a DksA-deficient E. coli host. Expression of the ant1 and kilA P1vir genes in the wild-type host was the highest among all and most probably leads to phage virulence. Interestingly, in a DksA-deficient host, P1vir genes encoding lysozyme and holin are downregulated, while antiholins are upregulated. Gene expression of RepA, a protein necessary for replication initiating at the phage oriR region, is increased in the dksA mutant; this is also true for phage genes responsible for viral morphogenesis and architecture. Still, it seems that P1vir is taking control of the bacterial protein, sugar, and lipid metabolism in both, the wild type and dksA− hosts. Generally, bacterial hosts are reacting by activating their SOS response or upregulating the heat shock proteins. However, only DksA-deficient cells upregulate their sulfur metabolism and downregulate proteolysis upon P1vir infection. We conclude that P1vir development is enhanced in the dksA mutant due to several improvements, including replication and virion assembly, as well as a less efficient lysis.