bluuebunny/crossref_metadata_2025 huggingface dataset:
Regional Right Ventricular Function Assessed by Intraoperative Three-Dimensional Echocardiography Is Associated With Short-Term Outcomes of Patients Undergoing Cardiac Surgery
Authors: Keller Marius , Duerr Marcia-Marleen , Heller Tim , Koerner Andreas , Schlensak Christian , Rosenberger Peter , Magunia Harry
Published: March 2022

Background

The assessment of right ventricular (RV) function in patients undergoing elective cardiac surgery is paramount for providing optimal perioperative care. The role of regional RV function assessment employing sophisticated state-of-the-art cardiac imaging modalities has not been investigated in this cohort. Hence, this study investigated the association of 3D echocardiography-based regional RV volumetry with short-term outcomes.

Materials and Methods

In a retrospective single-center study, patients undergoing elective cardiac surgery were included if they underwent 3D transesophageal echocardiography prior to thoracotomy. A dedicated software quantified regional RV volumes of the inflow tract, apical body and RV outflow tract employing meshes derived from 3D speckle-tracking. Echocardiographic, clinical and laboratory data were entered into univariable and multivariable logistic regression analyses to determine association with the endpoint (in-hospital mortality or the need for extracorporeal circulatory support).

Results

Out of 357 included patients, 25 (7%) reached the endpoint. Inflow RV ejection fraction (RVEF, 32 ± 8% vs. 37 ± 11%, p = 0.01) and relative stroke volume (rel. SV) were significantly lower in patients who reached the endpoint (44 ± 8 vs. 48 ± 9%, p = 0.02), while the rel. SV of the apex was higher (38 ± 10% vs. 33 ± 8%, p = 0.01). Global left and right ventricular function including RVEF and left ventricular global longitudinal strain did not differ. In univariable logistic regression, tricuspid regurgitation grade ≥ 2 [odds ratio (OR) 4.24 (1.66–10.84), p < 0.01], inflow RVEF [OR 0.95 (0.92–0.99), p = 0.01], inflow rel. SV [OR 0.94 (0.90–0.99), p = 0.02], apex rel. SV [OR 1.07 (1.02–1.13), p < 0.01] and apex to inflow rel. SV ratio [OR 5.81 (1.90–17.77), p < 0.01] were significantly associated with the endpoint. In a multivariable model, only the presence of tricuspid regurgitation [OR 4.24 (1.66–10.84), p < 0.01] and apex to inflow rel. SV ratio [OR 6.55 (2.09–20.60), p < 0.001] were independently associated with the endpoint.

Conclusions

Regional RV function is associated with short-term outcomes in patients undergoing elective cardiac surgery and might be helpful for optimizing risk stratification.
Fontan Circulation Associated Organ Abnormalities Beyond the Heart, Lungs, Liver, and Gut: A Systematic Review
Authors: Ritmeester Evi , Veger Veerle A. , van der Ven Jelle P. G. , van Tussenbroek Gabrielle M. J. W. , van Capelle Carine I. , Udink ten Cate Floris E. A. , Helbing Willem A.
Published: March 2022

Introduction

Patients with a Fontan circulation are at risk for sequelae of Fontan physiology during follow-up. Fontan physiology affects all organ systems and an overview of end-organ damage is needed.

Methods

We performed a systematic review of abnormalities in multiple organ systems for patients with a longstanding Fontan circulation. We searched online databases for articles describing abnormalities in multiple organ systems. Cardio-pulmonary abnormalities, protein losing enteropathy, and Fontan associated liver disease have already extensively been described and were excluded from this systematic review.

Results

Our search returned 5,704 unique articles. After screening, we found 111 articles relating to multiple organ systems. We found abnormalities in, among others, the nervous system, pituitary, kidneys, and musculoskeletal system. Pituitary edema—relating to the unique pituitary vasculature- may affect the thyroid axis. Renal dysfunction is common. Creatinine based renal function estimates may be inappropriate due to myopenia. Both lean muscle mass and bone mineral density are decreased. These abnormalities in multiple organ systems may be related to Fontan physiology, cyanosis, iatrogenic factors, or lifestyle.

Conclusions

Health care providers should be vigilant for hypothyroidism, visual or hearing deficits, and sleep disordered breathing in Fontan patients. We recommend including cystatin C for assessment of renal function. This review may aid health care providers and guide future research.

Systematic Review Registration:https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021232461, PROSPERO, identifier: CRD42021232461.
The Induction of Endothelial Autophagy and Its Role in the Development of Atherosclerosis
Authors: Hua Yunqing , Zhang Jing , Liu Qianqian , Su Jing , Zhao Yun , Zheng Guobin , Yang Zhihui , Zhuo Danping , Ma Chuanrui , Fan Guanwei
Published: March 2022

Increasing attention is now being paid to the important role played by autophagic flux in maintaining normal blood vessel walls. Endothelial cell dysfunction initiates the development of atherosclerosis. In the endothelium, a variety of critical triggers ranging from shear stress to circulating blood lipids promote autophagy. Furthermore, emerging evidence links autophagy to a range of important physiological functions such as redox homeostasis, lipid metabolism, and the secretion of vasomodulatory substances that determine the life and death of endothelial cells. Thus, the promotion of autophagy in endothelial cells may have the potential for treating atherosclerosis. This paper reviews the role of endothelial cells in the pathogenesis of atherosclerosis and explores the molecular mechanisms involved in atherosclerosis development.
The Epidemiology and Mechanisms of Lifetime Cardiopulmonary Morbidities Associated With Pre-Pregnancy Obesity and Excessive Gestational Weight Gain
Authors: Rastogi Shantanu , Rastogi Deepa
Published: March 2022

Obesity has reached pandemic proportions in the last few decades. The global increase in obesity has contributed to an increase in the number of pregnant women with pre-pregnancy obesity or with excessive gestational weight gain. Obesity during pregnancy is associated with higher incidence of maternal co-morbidities such as gestational diabetes and hypertension. Both obesity during pregnancy and its associated complications are not only associated with immediate adverse outcomes for the mother and their newborns during the perinatal period but, more importantly, are linked with long-term morbidities in the offsprings. Neonates born to women with obesity are at higher risk for cardiac complications including cardiac malformations, and non-structural cardiac issues such as changes in the microvasculature, e.g., elevated systolic blood pressure, and overt systemic hypertension. Pulmonary diseases associated with maternal obesity include respiratory distress syndrome, asthma during childhood and adolescence, and adulthood diseases, such as chronic obstructive pulmonary disease. Sequelae of short-term complications compound long-term outcomes such as long-term obesity, hypertension later in life, and metabolic complications including insulin resistance and dyslipidemia. Multiple mechanisms have been proposed to explain these adverse outcomes and are related to the emerging knowledge of pathophysiology of obesity in adults. The best investigated ones include the role of obesity-mediated metabolic alterations and systemic inflammation. There is emerging evidence linking metabolic and immune derangements to altered biome, and alteration in epigenetics as one of the intermediary mechanisms underlying the adverse outcomes. These are initiated as part of fetal adaptation to obesity during pregnancy which are compounded by rapid weight gain during infancy and early childhood, a known complication of obesity during pregnancy. This newer evidence points toward the role of specific nutrients and changes in biome that may potentially modify the adverse outcomes observed in the offsprings of women with obesity.
Late Quaternary Drainage Rearrangement Prevents the Vegetation Development in the La Tatacoa Intermontane Basin of the Colombian Andes
Authors: Souza D. H. , Parra M. , del Rio I. , Sawakuchi A. O. , Pupim F. N. , Hernández-González J. S. , Gomez S.
Published: March 2022

The Tatacoa Desert (TD) is a Cenozoic basin between the Central and Eastern Cordilleras in the southern Colombian Andes. Its sparse vegetation and Badlands morphology resemble a semi-arid environment, despite receiving ∼1,300 mm of mean annual precipitation. Based on optically stimulated luminescence dating in two fluvial terraces and drainage morphometric analysis, we propose that such “aridification” was imposed by a drainage reorganization during the Late Pleistocene. No river coming from the neighboring mountains flows into the TD. This makes the Tatacoa rivers unable to balance the evapotranspiration effect, causing a surficial water deficit. A fluvial terrace (TAT-2) works as a divide between the Tatacoa catchments and the Cabrera River, the latter coming from the Eastern Cordillera and bounding the TD towards the north. Paleocurrent measurements on its sedimentary lithic clasts point to a W-NW-directed flow into the TD. Thus, OSL ages around 74.1 ± 7.0 ka in this terrace suggest the TD was drained by an Eastern Cordillera catchment during the Late Pleistocene. At the western end of the TD, a dissected fluvial terrace (TAT-1) lying 80 m above the Magdalena River ages between 6.3 ± 1.5 and 7.7 ± 1.5 ka, representing the Holocene incision rates of 10.8 ± 0.3 mm/y. Coupled OSL data and river longitudinal profile analysis show a high susceptibility to erosion of the Tatacoa rocks and estimate incision rates ranging from 2–5 mm/y to 10–20 mm/y within the TD. The higher rates are concentrated along knickpoints related to anticlines and thrust faults. Thus, a northward shift of the Cabrera River and a westward migration of the Magdalena Valley have drastically reduced the surficial water availability in the Tatacoa and the development of vegetation, increasing erodibility. Potential climatic and tectonic drivers include 1) terrace accumulation during the wet periods of Marine Isotope Stage 5A and 1; 2) fault-induced incision of the Magdalena River in the NW, which would have favored northward migration of tributaries such as the Cabrera River; and 3) activity on the Baraya thrust fault, which would have prevented rivers from the Eastern Cordillera to enter the desert.
Sandstone Slope Stability Analysis Under Wetting-Drying Cycles Based on Generalized Hoek-Brown Failure Criterion
Authors: Kang Kai , Huang Shiyuan , Liu Wenhua , Cheng Hu , Fomenko Igor , Zhou Yuxin
Published: March 2022

The deterioration of rock strength due to cyclic fluctuations in the groundwater level in drawdown regions influences bank slope stability. Based on wetting–drying cycling and uniaxial compression tests of sandstone specimens, the intact rock strength deterioration characteristics were analyzed. Considering different types of rock mass discontinuities, the rock mass strength deterioration features were shown. The results of rock mass strength deterioration characteristics were then applied in sandstone slope stability analysis. Using the finite element method, the safety factors of the slope under different wetting–drying cycles were computed based on the generalized Hoek–Brown failure criterion. Meanwhile, statistical distribution features of strength parameters were considered, and safety factor distribution of studied slopes was obtained with Monte Carlo simulation. The results show that rock mass deformation modulus decreased during wetting–drying cycling. The mean safety factors of the slope with any type of discontinuities were reduced by wetting–drying cycling in probabilistic analyses. Wetting–drying cycling plays an important role in damaging slope stability at initial stages since the degree of rock mass strength deterioration is remarkable during initial wetting–drying cycles. However, the rock mass strength and safety factor of the slope are reduced gently after 10 wetting–drying cycles. This research presents the phases of the effect of wetting–drying cycles on sandstone slope stability in drawdown regions.
Solution-Processed Titanium Dioxide Ion-Gated Transistors and Their Application for pH Sensing
Authors: Subramanian Arunprabaharan , Azimi Mona , Leong Cheng Yee , Lee Siew Ling , Santato Clara , Cicoira Fabio
Published: March 2022

Titanium dioxide (TiO2) is an abundant metal oxide, widely used in food industry, cosmetics, medicine, water treatment and electronic devices. TiO2 is of interest for next-generation indium-free thin-film transistors and ion-gated transistors due to its tunable optoelectronic properties, ambient stability, and solution processability. In this work, we fabricated TiO2 films using a wet chemical approach and demonstrated their transistor behavior with room temperature ionic liquids and aqueous electrolytes. In addition, we demonstrated the pH sensing behavior of the TiO2 IGTs with a sensitivity of ∼48 mV/pH. Furthermore, we demonstrated a low temperature (120°C), solution processed TiO2-based IGTs on flexible polyethylene terephthalate (PET) substrates, which were stable under moderate tensile bending.
Estrogen and Estrogen Receptor Modulators: Potential Therapeutic Strategies for COVID-19 and Breast Cancer
Authors: Hu Shuying , Yin Feiying , Nie Litao , Wang Yuqin , Qin Jian , Chen Jian
Published: March 2022

Owing to the ongoing coronavirus disease 2019 (COVID-19) pandemic, we need to pay a particular focus on the impact of coronavirus infection on breast cancer patients. Approximately 70% of breast cancer patients express estrogen receptor (ER), and intervention therapy for ER has been the primary treatment strategy to prevent the development and metastasis of breast cancer. Recent studies have suggested that selective estrogen receptor modulators (SERMs) are a potential therapeutic strategy for COVID-19. With its anti-ER and anti-viral combined functions, SERMs may be an effective treatment for COVID-19 in patients with breast cancer. In this review, we explore the latent effect of SERMs, especially tamoxifen, and the mechanism between ER and virus susceptibility.
Analysis of Abnormal Detection Data of Fire Accident in Power Cable Tunnel and Field Test Study on Characteristic Parameters of Tunnel Fire
Authors: Guo Wei , Pu Ziheng , Ren Zhigang , Zhou Shiyi , Quan Lang , Men Yekun , Pan Zehua
Published: March 2022

With the continuous expansion of urban cable scale and the increase in service time, the fire accident risk of cable tunnels also increases. Defects in the configuration of fire prevention measures lead to many fire accidents and seriously affect the reliability of power supply. First, the detection data of a typical tunnel fire accident are analyzed in this study. The results show that the main problems are the unreasonable installation position of fire extinguishers, the lack of fire prevention measures between intermediate joints, and the insufficient fire prevention performance of interlayer fire barriers. Then, the field test of cable tunnel fire is carried out in a cable tunnel to be put into operation. The test data show that the high-temperature area is mainly concentrated on the top of the tunnel, and the insufficient suspension height of the fire extinguisher will affect the trigger time. The energy of a short circuit accident is large, the cable burns and spreads rapidly, and the delayed triggering may lead to large fire to extinguish effectively. Comparing the simulation and test results of abnormal characteristic parameters in fire, the temperature error is less than 4%. The fire power development curve is obtained through simulation, and the burner power is set to test the fire prevention and extinguishing devices. Finally, the optimal configuration scheme of the fire anomaly data detection equipment and fire-extinguishing products is proposed. The temperature drop rate increases by 135%.
Ecologic Service, Economic Benefits, and Sustainability of the Man-Made Ecosystem in the Taklamakan Desert
Authors: Congjuan Li , Abulimiti Madinai , Jinglong Fan , Haifeng Wang
Published: March 2022

The longest artificial highway shelterbelt flowing across the Taklamakan desert was conducted in 2005. To ensure the sustainable development of the highway and shelterbelts, scholars have carried out a large number of studies on the shelterbelt since 1991. This review is based on a 30-year monitoring program from 1991 to 2021. We find that, with the increase of shelterbelt age, some ecological and economic benefits gradually appear, such as saving transportation cost, promoting economic development in Southern Xinjiang, improving the sandy soil and microenvironment, and increasing the biodiversity. In the meanwhile, some adverse phenomena also exist, such as high maintenance costs, decrease in water tables because of irrigation, threats of the protected species, pollution from pumping oil wells, and the potential damage from highway burying as sand dunes move forward. In view of these problems, some suggestions are as follows. First, the science-policy sections of both the UNFCCC and the UNCCD should pay special attention to providing research-based guidance for the international cooperation and coordination. Second, China government should provide the necessary funding to establish the 27th China desert observation and research station to ensure long-term monitoring and slow down the pace of construction on the desert highway to allow time to develop strategies that minimize the impact on wild animals. Third, it is necessary to apply solar photovoltaic power in well irrigation and sand industry development. Fourth, the sand-blocking belt of 10 m width and 10 m away from the outside of the upwind shelterbelt can be laid every five years in view of sand-buried roads. In short, the inter-agency collaboration and coordination are both essential to ensuring the success of sustainable development and management of the Taklamakan Desert man-made ecosystem.
Community-Based Conservation and Management of Chelonians in the Amazon
Authors: Andrade Paulo Cesar Machado , de Oliveira Paulo Henrique Guimarães , de Lima Aldeniza Cardoso , da Mota Duarte João Alfredo , da Silva Azevedo Sandra Helena , de Oliveira Anndson Brelaz , de Almeida Carlos Dias , da Silva Eleyson Barboza , Garcez Jânderson Rocha , da Silva Pinto José Ribamar , da Silva Liriann Chrisley Nascimento , Monteiro Midian Salgado , da Silva Rodrigues Wander , Anízio Thiago Luiz Ferreira , Pontes Alfredo Luiz Belém , Teixeira Ruth Lima , da Silva Jefferson Moreira , Duncan Wallice Luiz Paxiúba , Vogt Richard Carl
Published: March 2022

Chelonians represent an important resource in the Amazon, either as a source of protein at the base of the food chain of aquatic and transition ecosystems, or in the dispersion of seeds of plants from floodplains and flooded forests. The consumption and predatory exploitation of their meat and eggs by local populations has been, and still is, one of the main threats to these animals. Community-based conservation projects allied to official protection programs have been restoring populations of chelonians of the genus Podocnemis throughout the Amazon since 1974. In this study, we analyzed the historical time series of protection data of Podocnemis expansa, P. unifilis, P. sextuberculata and P. erythrocephala in areas protected by the government and communities in the Amazonas state and northwest of Pará state. Between 1974 and 2019, 230,444 nests and 21,350,201 hatchlings of P. expansa, 170,076 nests and 3,229,821 hatchlings of P. unifilis, 647,715 nests and 6,410,092 hatchlings of P. sextuberculata and 24,617 nests and 168,856 hatchlings of P. erythrocephala were protected. Community protection schemes emerged in 1990, and covered 80.7% of the areas and produced 64.2% of P. unifilis hatchlings and 44.6% of P. sextuberculata hatchlings. The areas with the highest production of P. expansa remain under government protection (57.4%). Using the time series of production of nests and hatchlings per beach, logistic growth curves were estimated, and the values of r and K were compared between the two protection systems (government and community). Beaches controlled by the government showed higher support capacity in the production of nests (1,910.7 ± 1,035) and hatchlings (211,513 ± 93,031) of P. expansa and P. sextuberculata (81,160 ± 34,924 hatchlings). However, the communities were more efficient in protecting nests (r = 0.102 ± 0.2315) and hatchlings (r = 0.282 ± 0.166) of P. unifilis. Community-based protection and monitoring programs are an important component that should be incorporated by the government’s environmental agencies for the management and conservation of turtles in the Amazon.
Sexual Selection May Not Often Reduce Gene Flow Between Locally Adapted Populations. A Review of Some Evidence, and Suggestions for Better Tests
Authors: Edelaar Pim
Published: March 2022

Sexually selected traits often depend on an individual’s physical condition, or otherwise indirectly reflect the ecological performance of individuals. When individuals disperse between populations that are locally adapted to different environments, their ecological performance may decline. This in turn may result in more poorly expressed sexual traits, and therefore in a lower reproductive success. Hence, sexual selection may reduce the effective gene flow between populations, and thereby maintain or even enhance population divergence. This hypothesis was published in a highly visible journal (van Doorn et al., 2009, Science). Here I review the subsequently published empirical tests of this hypothesis. I downloaded all metadata (incl. abstracts) of papers citing van Doorn et al. (2009) and read those papers that undertook relevant tests. To my surprise, only very few papers provided explicit tests of the hypothesis, this never involved plants, and only one study found support for it. While sexual selection may therefore not often reduce gene flow between locally adapted populations, some improvements to experimental design and choice of study system are noted. I therefore also provide a detailed list of suggestions for high quality tests of this hypothesis. This hopefully acts as a catalyst for more and better studies to test whether sexual and natural selection can work in synergy to reduce effective dispersal, and thereby protect and promote adaptive population divergence.
Parapatric Genetic Lineages Persist in a Multiply Introduced Non-native Bush-Cricket
Authors: Kaňuch Peter , Cassel-Lundhagen Anna , Preuss Sonja , Nordlander Göran , Berggren Åsa
Published: March 2022

To understand colonization success of an invasive species we need to know the origin of the founders, where and when they were introduced, and how they spread from the introduction site(s) through the landscape. Admixture of different genetic lineages from multiple introductions is generally hypothesized to be beneficial to invasive species thanks to adaptive variation and heterozygosity-fitness correlations. In this study, population genetic and landscape data was gathered for Roesel’s bush-cricket,Roeseliana roeseliia small bush-cricket common in central and eastern Europe that currently is expanding its range in northern Europe. We examined how colonization history and landscape structure affect the spread of the species and its population genetic structure, as a consequence of multiple introductions. Using comprehensive information of the species ecology and dispersal, together with genetic structure inferred from samples from 29 locations in central Sweden (we employed data published by Preuss et al., 2015), we found that two parapatric founding lineages have coexisted with very little gene flow during a long time span. An isolation-by-distance pattern and a decrease of genetic diversity toward marginal areas were more pronounced in the lineage situated in forest dominated landscapes. Our findings are in strong contrast to the hypothesis that different genetic lineages will admix when introduced to the same area. The presence of the separate lineages decades after introduction and without physical barriers for gene flow shows that some mechanism prevents them from admixture. One possibility is that the lineages with different genetic setups have adapted independently to local conditions and their admixture resulted in loss of locally adapted genotypes and hybrid offspring, less viable than the respective ancestral genotypes. However, an alternative post-mating reproductive barrier and hybrid breakdown phenomenon should also be considered. Our data indicate that besides landscape characteristics, human transportation of agricultural goods may play an important role for the overall spatial genetic pattern of the species in the study area by aiding the spread of the species.
The Ovarian Transcriptome at the Early Stage of Testis Removal-Induced Male-To-Female Sex Change in the Protandrous Black Porgy Acanthopagrus schlegelii
Authors: Tseng Peng-Wei , Wu Guan-Chung , Kuo Wei-Lun , Tseng Yung-Che , Chang Ching-Fong
Published: March 2022

Unlike gonochoristic fishes, sex is fixed after gonadal differentiation (primary sex determination), and sex can be altered in adults (secondary sex determination) of hermaphroditic fish species. The secondary sex determination of hermaphroditic fish has focused on the differences between testicular tissue and ovarian tissue during the sex change process. However, comprehensive studies analyzing ovarian tissue or testicular tissue independently have not been performed. Hermaphroditic black porgy shows a digonic gonad (ovarian tissue with testicular tissue separated by connective tissue). Protandrous black porgy has stable maleness during the first two reproductive cycles (<2 years old), and approximately 50% enter femaleness (natural sex change) during the third reproductive cycle. Precocious femaleness is rarely observed in the estradiol-17β (E2)-induced female phase (oocytes maintained at the primary oocyte stage), and a reversible female-to-male sex change is found after E2 is withdrawn in <2-year-old fish. However, precocious femaleness (oocytes entering the vitellogenic oocyte stage) is observed in testis-removed fish in <2-year-old fish. We used this characteristic to study secondary sex determination (femaleness) in ovarian tissue via transcriptomic analysis. Cell proliferation analysis showed that BrdU (5-bromo-2′-deoxyuridine)-incorporated germline cells were significantly increased in the testis-removed fish (female) compared to the control (sham) fish (male) during the nonspawning season (2 months after surgery). qPCR analysis showed that there were no differences in pituitary-releasing hormones (lhb and gtha) in pituitary and ovarian steroidogenesis-related factors (star, cyp11a1, hsd3b1, and cyp19a1a) or female-related genes (wnt4a, bmp15, gdf9, figla, and foxl2) in ovarian tissues between intact and testis-removed fish (2 months after surgery). Low expression of pituitary fshb and ovarian cyp17a1 was found after 2 months of surgery. However, we did find small numbers of genes (289 genes) showing sexual fate dimorphic expression in both groups by transcriptomic analysis (1 month after surgery). The expression profiles of these differentially expressed genes were further examined by qPCR. Our present work identified several candidate genes in ovarian tissue that may be involved in the early period of secondary sex determination (femaleness) in black porgy. The data confirmed our previous suggestion that testicular tissue plays an important role in secondary sex determination in protandrous black porgy.
Comprehensive Analysis of YTHDF1 Immune Infiltrates and ceRNA in Human Esophageal Carcinoma
Authors: Liu Xu-Sheng , Kui Xue-Yan , Gao Yan , Chen Xue-Qin , Zeng Jing , Liu Xiao-Yu , Zhang Yu , Zhang Yao-Hua , Pei Zhi-Jun
Published: March 2022

Background: YTHDF1 is highly expressed in multiple tumors and affects tumor progression. However, there are only a few comprehensive studies on the analysis of YTHDF1 in esophageal cancer.

Methods: We analyzed YTHDF1 expression in pan-cancer by comparing both the GEPIA and TCGA cohorts, and further verified the differences in YTHDF1 expression between the ESCA and normal groups by the GEO ESCA cohort and in vitro experiments. The correlation of YTHDF1 expression and the clinical characteristics of ESCA patients was analyzed using the TCGA ESCA clinical data. The GO and KEGG enrichment analyses of the YTHDF1 coexpressed genes were completed by bioinformatics analysis, and the GGI and PPI were constructed for the YTHDF1, respectively. The relationship between YTHDF1 expression and the infiltration of ESCA immune cells was analyzed by using the TIMER database and the TCGA ESCA cohort. The relationships between YTHDF1 expression levels and glycolysis and ferroptosis-related genes were analyzed using the TCGA and GEPIA ESCA cohorts. Finally, the ceRNA network that may be involved in YTHDF1 in ESCA was predicted and constructed through a variety of databases.

Results: YTHDF1 was overexpressed in various cancers, and in vitro experiments confirmed that YTHDF1 expression was higher in ESCA samples than in normal samples. The expression of YTHDF1 has some accuracy in predicting the tumor outcome. Expression of YTHDF1 was significantly associated with multiple clinical features in ESCA patients. GO and KEGG enrichment analyses indicated that YTHDF1 coexpressed genes involved multiple biological functions. There is a potential association between YTHDF1 expression and multiple immune cell infiltration, glycolysis, and ferroptosis-related genes in ESCA. YTHDF1 may be involved in multiple ceRNA regulatory networks in ESCA, including PAXIP1-AS1/hsa-miR-376c-3p/YTHDF1 axis, THUMPD3-AS1/hsa-miR-655-3p/YTHDF1 axis, and SNHG20/hsa-miR-655-3p/YTHDF1 axis, respectively.

Conclusion: YTHDF1 can serve as a biomarker of ESCA, related to the immune cell infiltration of ESCA, regulation of glycolysis and ferroptosis, and the ceRNA regulatory network.
Role of Oxidative Stress in Varicocele
Authors: Wang Kaixian , Gao Yuanyuan , Wang Chen , Liang Meng , Liao Yaping , Hu Ke
Published: March 2022

According to the official statistics of the World Health Organization, at least 48 million couples and 186 million people suffer from infertility. Varicocele has been recognized as the leading cause of male infertility and can affect spermatogenesis and cause testicular and epididymal disorders through multiple diverse pathophysiological processes. Reactive oxygen species (ROS) produced by oxidative stress have been reconciled as an important pathogenic factor throughout the course of varicocele. Testis respond to heat stress, hypoxia, and inflammation at the cost of producing excessive ROS. High levels of ROS can lead to infertility not only through lipid peroxidation or DNA damage, but also by inactivating enzymes and proteins in spermatogenesis. This review studies the oxidative stress and its role in the pathophysiology and molecular biology of varicocele in the context of a decline in fertility.
Advances in Nanotechnology Development to Overcome Current Roadblocks in CAR-T Therapy for Solid Tumors
Authors: Mi Juan , Ye Qing , Min Yuanzeng
Published: March 2022

Chimeric antigen receptor T cell (CAR-T) therapy for the treatment of hematologic tumors has achieved remarkable success, with five CAR-T therapies approved by the United States Food and Drug Administration. However, the efficacy of CAR-T therapy against solid tumors is not satisfactory. There are three existing hurdles in CAR-T cells for solid tumors. First, the lack of a universal CAR to recognize antigens at the site of solid tumors and the compact tumor structure make it difficult for CAR-T cells to locate in solid tumors. Second, soluble inhibitors and suppressive immune cells in the tumor microenvironment can inhibit or even inactivate T cells. Third, low survival and proliferation rates of CAR-T cells in vivo significantly influence the therapeutic effect. As an emerging method, nanotechnology has a great potential to enhance cell proliferation, activate T cells, and restarting the immune response. In this review, we discuss how nanotechnology can modify CAR-T cells through variable methods to improve the therapeutic effect of solid tumors.
Escaping Death: How Cancer Cells and Infected Cells Resist Cell-Mediated Cytotoxicity
Authors: Tuomela Karoliina , Ambrose Ashley R. , Davis Daniel M.
Published: March 2022

Cytotoxic lymphocytes are critical in our immune defence against cancer and infection. Cytotoxic T lymphocytes and Natural Killer cells can directly lyse malignant or infected cells in at least two ways: granule-mediated cytotoxicity, involving perforin and granzyme B, or death receptor-mediated cytotoxicity, involving the death receptor ligands, tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) and Fas ligand (FasL). In either case, a multi-step pathway is triggered to facilitate lysis, relying on active pro-death processes and signalling within the target cell. Because of this reliance on an active response from the target cell, each mechanism of cell-mediated killing can be manipulated by malignant and infected cells to evade cytolytic death. Here, we review the mechanisms of cell-mediated cytotoxicity and examine how cells may evade these cytolytic processes. This includes resistance to perforin through degradation or reduced pore formation, resistance to granzyme B through inhibition or autophagy, and resistance to death receptors through inhibition of downstream signalling or changes in protein expression. We also consider the importance of tumour necrosis factor (TNF)-induced cytotoxicity and resistance mechanisms against this pathway. Altogether, it is clear that target cells are not passive bystanders to cell-mediated cytotoxicity and resistance mechanisms can significantly constrain immune cell-mediated killing. Understanding these processes of immune evasion may lead to novel ideas for medical intervention.
Bioprospecting for Microorganisms in Peloids—Extreme Environment Known for Its Healing Properties
Authors: Vadlja Denis , Bujak Maro , Čož-Rakovac Rozelindra , Roje Marin , Čižmek Lara , Horvatić Anita , Svetličić Ema , Diminić Janko , Rakovac Saša , Oros Damir , Zucko Jurica , Starcevic Antonio
Published: March 2022

Seawater is an environment in which numerous microorganisms have evolved, some with a great potential for biotechnology. In recent years, many scientists have moved away from the assumption that the origin of life was in pools of water, and instead propose that life on Earth probably originated in accumulations of warm, nutrient-rich mud. This mud, also called peloid is a rich source of organisms that, due to their adaptation to this unique environment, produce a wide variety of primary and secondary metabolites with numerous and diverse activities, including anti-cancer, anti-inflammatory, antiviral, and immunomodulatory ones. In this research, two questions were addressed using collected samples of a peloid with demonstrated healing properties. Firstly biodiversity in this ecological niche was explored in order to assess microbial communities present and secondly natural products were screened for in order to assess whether predicted activities could be linked to healing properties of the peloid. The use of peloids in medical therapy dates back to ancient times. Abiotic components such as clay and mineral water are believed to be the main contributors of the healing properties of natural peloids. The places where peloids are usually found are characteristically shallow and enclosed lagoons. The constant UV exposure and increased salt concentration classify peloid as an extreme environment. The spectrum of relief’s peloid therapy is claimed to provide ranges from purely cosmetic and skin-related to musculoskeletal and immunological problems. These claims can hardly be supported by mineral content and heat-retaining properties alone. However, organic compounds from present microorganisms as well as secondary metabolites could help explain the observed range of health benefits. The fact that the relationship between the therapeutic activity of peloids and their composition besides mineral and physicochemical properties has not been extensively studied indicates untapped biotechnological potential.
Anthropogenic Mixing in Seasonally Stratified Shelf Seas by Offshore Wind Farm Infrastructure
Authors: Dorrell Robert M. , Lloyd Charlie J. , Lincoln Ben J. , Rippeth Tom P. , Taylor John R. , Caulfield Colm-cille P. , Sharples Jonathan , Polton Jeff A. , Scannell Brian D. , Greaves Deborah M. , Hall Rob A. , Simpson John H.
Published: March 2022

The offshore wind energy sector has rapidly expanded over the past two decades, providing a renewable energy solution for coastal nations. Sector development has been led in Europe, but is growing globally. Most developments to date have been in well-mixed, i.e., unstratified, shallow-waters near to shore. Sector growth is, for the first time, pushing developments to deep water, into a brand new environment: seasonally stratified shelf seas. Seasonally stratified shelf seas, where water density varies with depth, have a disproportionately key role in primary production, marine ecosystem and biogeochemical cycling. Infrastructure will directly mix stratified shelf seas. The magnitude of this mixing, additional to natural background processes, has yet to be fully quantified. If large enough it may erode shelf sea stratification. Therefore, offshore wind growth may destabilize and fundamentally change shelf sea systems. However, enhanced mixing may also positively impact some marine ecosystems. This paper sets the scene for sector development into this new environment, reviews the potential physical and environmental benefits and impacts of large scale industrialization of seasonally stratified shelf seas and identifies areas where research is required to best utilize, manage, and mitigate environmental change.
Forced vs. Intrinsic Wintertime Submonthly Variability of Sea Surface Temperature in the Midlatitude Western North Pacific
Authors: Wu Yusheng , Zhou Guidi , Wang Guifen , Cheng Xuhua
Published: March 2022

The relative importance of wintertime forced and intrinsic SST variability in the Kuroshio-Oyashio Extension (KOE) region on submonthly timescales (2–10 and 10–30 days) is evaluated based on theoretical, observational, and modeling analysis. It is shown that the theoretical framework extended from the stochastic climate model has difficulties in representing observed SST variability on such short scales. We then employ the single-column General Ocean Turbulence Model (GOTM) to explicitly evaluate the SST variability forced by atmospheric disturbances. Results show that in the KOE region forced SST variability is responsible for a very small fraction of the total variability (<10%) on the submonthly scales, indicating the dominance of intrinsic oceanic processes. Outside the KOE forced variability dominates. By means of sensitivity experiments, the key physical factors are identified: upper ocean vertical mixing, wind stress forcing (mainly for outside KOE), and latent heat flux, the former two of which are not considered in the theoretical framework. The above results are robust against different levels of submonthly SST variability.
Discrepant Effects of Oceanic Advection in the Evolution of SST Anomalies in the South China Sea During El Niño of Different Intensities
Authors: Xiao Fuan , Wang Dongxiao , Wu Qiaoyan , Song Wei , Zeng Lili , Xie Qiang , Wang Yan
Published: March 2022

Observed different evolutions of SST anomalies in the South China Sea (SCS) between super and other El Niño events are revealed. The results suggest that the first (second) warming peak is warmer than the second (first) one for super (other) El Niño composite. Mixed layer heat budget analysis indicates that during the first warming period, the vertical advection induced by the anomalous anticyclone in the SCS (SCSAC) warms the SST in the central SCS through the basin-scale downwelling motion for super El Niño composite. In contrast, the positive shortwave radiation anomalies from atmosphere into ocean associated with the reduced total cloud cover contribute the most warming SST for other El Niño composite. During the cooling period, the horizontal linear cold advection associated with a large west-east SST gradient anomaly and climatological western boundary currents plays the most important role in rapid cooling SST for super El Niño composite. However, the nonlinear warm advection associated with the northward current anomalies and anomalous SST gradient in the western SCS maintains the SCS SST for other El Niño composite. During the second warming period, the net surface heat flux anomalies act as a damping process, while the warming process could be attributed to the vertical warm advection. Specially, the SCSAC weakens but the anomalous easterly winds strengthen to suppress the upwelling off the coast of southeast Vietnam for other El Niño composite. The anomalous ocean circulations and associated advections are related to the development of SCSAC, which could be largely attributed to the SST anomalies in the Indian Ocean. In contrast with previous studies, our results highlight the role of ocean dynamics in the evolution of SCS SST anomalies during El Niño of different intensities.
The Association of Hyperuricemia and Gout With the Risk of Cardiovascular Diseases: A Cohort and Mendelian Randomization Study in UK Biobank
Authors: Zhu Jianwei , Zeng Yu , Zhang Hanyue , Qu Yuanyuan , Ying Zhiye , Sun Yajing , Hu Yao , Chen Wenwen , Yang Huazhen , Yang Jing , Song Huan
Published: March 2022

Background

The association between hyperuricemia/gout with cardiovascular diseases (CVD) have been investigated. However, whether the magnitude of associations differs between hyperuricemia and gout, and the causality of these associations, remains inconclusive.

Methods

Based on UK Biobank, we conducted a cohort analysis including 431,967 participants, who were categorized as gout, hyperuricemia, and normal groups at recruitment, and followed up for CVD until December 2019. The phenotypic association of hyperuricemia/gout with CVD was estimated by Cox regression, adjusting for multiple confounders. Further exploration on the causality of such links was performed using Mendelian Randomization (MR) analysis, where we selected exclusive genetic variants for hyperuricemia and for gout based on summary GWAS data from independent populations.

Results

During mean 10.20 years of follow-up, hyperuricemia patients were associated with increased CVD (HR = 1.33, 95% CI: 1.29–1.36), compared to individuals who were free of hyperuricemia/gout. The risk elevation was even higher for gout patients (HR = 1.54, 95% CI: 1.48–1.62). Furthermore, we found significantly positive association between genetic liability for hyperuricemia and CVD in both one-sample (OR = 1.06, 95% CI: 1.02–1.11) and two-sample (OR = 1.09, 95% CI: 1.03–1.16) MR analysis. However, genetic liability for gout was not associated with CVD (OR = 0.89, 95% CI: 0.79–1.01 in one-sample, and OR = 0.92, 95% CI: 0.82–1.21 in two-sample MR analysis).

Conclusion

Individuals with hyperuricemia/gout were at increased risk of various types of CVD. As the MR analyses suggest a causal effect of hyperuricemia, but not gout, on CVD, these results indicate the possible effects of other gout-associated factors on the development of CVD, in addition to the uric acid pathway.
Control of a COVID-19 Outbreak in a Spanish Prison: Lessons Learned in Outbreak Control
Authors: Vicente-Alcalde Nancy , Ruescas-Escolano Esther , Franco-Paredes Carlos , Tuells José
Published: March 2022

The rapid spread of highly transmissible respiratory infections in carceral settings occurs due to their conglomerate nature. The COVID-19 pandemic has resulted in large outbreaks in jails and prisons in many settings. Herein, we describe an outbreak of SARS-CoV2 infection in a prison in Alicante, Spain. Prior to January 2021, testing for coronavirus infection was not widely available in jails and prisons nationwide. Offering of testing services in Spanish carceral facilities, coincided with the deployment of COVID-19 vaccination in the larger community. However, COVID-19 vaccine role out of incarcerated individuals occurred later during the deployment plan. With the identification of the initial cases of this outbreak, two units of the facility were assigned for population management: one for inmates with confirmed infection by positive PCR detection of SARS-COV-2 infection in nasopharyngeal swabs. Inmates with confirmed exposure and thus considered close contacts were place in a second isolation unit. Functional quarantine was employed in some instances. A reactive testing strategy was instituted at baseline, and at 7 and 14 days of nasopharyngeal specimens by PCR. A total of 1,097 nasopharyngeal specimens were obtained for PCR testing during the outbreak, which lasted a total of 80 days between the index case the end of medical isolation of the last case. A total of 103 COVID-19 cases were identified during the outbreak. Of these, three inmates developed severe manifestations requiring hospitalization, and one died. Were identified, among which there were three hospitalized and one deceased. Among cases and confirmed contacts, we conducted close clinical monitoring, symptom screening, and daily temperature checks. The implementation of these interventions along with early medical isolation of cases, quarantining of contacts, and interval testing to detect presymptomatic or asymptomatic cases were instrumental in containing this outbreak.
Development and Validation of an Artificial Intelligence Preoperative Planning System for Total Hip Arthroplasty
Authors: Chen Xi , Liu Xingyu , Wang Yiou , Ma Ruichen , Zhu Shibai , Li Shanni , Li Songlin , Dong Xiying , Li Hairui , Wang Guangzhi , Wu Yaojiong , Zhang Yiling , Qiu Guixing , Qian Wenwei
Published: March 2022

Background

Accurate preoperative planning is essential for successful total hip arthroplasty (THA). However, the requirements of time, manpower, and complex workflow for accurate planning have limited its application. This study aims to develop a comprehensive artificial intelligent preoperative planning system for THA (AIHIP) and validate its accuracy in clinical performance.

Methods

Over 1.2 million CT images from 3,000 patients were included to develop an artificial intelligence preoperative planning system (AIHIP). Deep learning algorithms were developed to facilitate automatic image segmentation, image correction, recognition of preoperative deformities and postoperative simulations. A prospective study including 120 patients was conducted to validate the accuracy, clinical outcome and radiographic outcome.

Results

The comprehensive workflow was integrated into the AIHIP software. Deep learning algorithms achieved an optimal Dice similarity coefficient (DSC) of 0.973 and loss of 0.012 at an average time of 1.86 ± 0.12 min for each case, compared with 185.40 ± 21.76 min for the manual workflow. In clinical validation, AIHIP was significantly more accurate than X-ray-based planning in predicting the component size with more high offset stems used.

Conclusion

The use of AIHIP significantly reduced the time and manpower required to conduct detailed preoperative plans while being more accurate than traditional planning method. It has potential in assisting surgeons, especially beginners facing the fast-growing need for total hip arthroplasty with easy accessibility.