bluuebunny/crossref_metadata_2025 huggingface dataset:
MADS Based on DL Techniques on the Internet of Things (IoT): Survey
Authors: Talal Hussah , Zagrouba Rachid
Published: October 2021

Technologically speaking, humanity lives in an age of evolution, prosperity, and great development, as a new generation of the Internet has emerged; it is the Internet of Things (IoT) which controls all aspects of lives, from the different devices of the home to the large industries. Despite the tremendous benefits offered by IoT, still there are some challenges regarding privacy and information security. The traditional techniques used in Malware Anomaly Detection Systems (MADS) could not give us as robust protection as we need in IoT environments. Therefore, it needed to be replaced with Deep Learning (DL) techniques to improve the MADS and provide the intelligence solutions to protect against malware, attacks, and intrusions, in order to preserve the privacy of users and increase their confidence in and dependence on IoT systems. This research presents a comprehensive study on security solutions in IoT applications, Intrusion Detection Systems (IDS), Malware Detection Systems (MDS), and the role of artificial intelligent (AI) in improving security in IoT.
Climate and Management Factors Underlying Changes in Beech Forest Herbaceous Layer Plant Communities in the Polish Eastern Carpathians
Authors: Bugno-Pogoda Anna , Durak Tomasz
Published: October 2021

The herbaceous vegetation and forest stand characteristics in European beech forests growing in the Polish part of the Eastern Carpathians have changed over the last 40 years. This has been influenced by many factors, including land-use change, forest management and climate change. This study investigates changes in forest cover and structure and the associated changes in herbaceous layer plant communities and seeks to elucidate whether and how beech forest herbaceous layer communities have been affected by climate change. The study used information from archival and current land cover maps, semi-permanent sampling plots, forest management plans for the Forest Districts of Brzozów, Lesko and Ustrzyki Dolne and meteorological weather station data compiled for three study periods of herbaceous vegetation (1970s, 2000s, 2010s). In the study area, the regular shelterwood system was changed to an irregular shelterwood system that produces stands with a complex overstorey structure. The results revealed the important role of light availability in shaping the species composition of the herbaceous layer in semi-natural Carpathian beech forests, which was strongly related to the course of management activities. An overall decrease in the number of species during the 2010s is linked to the ageing of beech forests, increased intensity of management activities in ageing stands, competition from understorey vegetation and lower soil moisture that can be linked to climate change. Our study partially supports the existing findings that more manipulative forest management systems can play an important role in countering the current and expected effects of climate change on the forest ecosystem because of the low degree of spatial differentiation of the stand’s structure (developmental stages). Therefore, foresters managing the structure of stands should strive to create a forest structure with high variability of developmental stages on a regional scale.
Electric Bicyclist Injury Severity during Peak Traffic Periods: A Random-Parameters Approach with Heterogeneity in Means and Variances
Authors: Zhu Tong , Zhu Zishuo , Zhang Jie , Yang Chenxuan
Published: October 2021

Accidents involving electric bicycles, a popular means of transportation in China during peak traffic periods, have increased. However, studies have seldom attempted to detect the unique crash consequences during this period. This study aims to explore the factors influencing injury severity in electric bicyclists during peak traffic periods and provide recommendations to help devise specific management strategies. The random-parameters logit or mixed logit model is used to identify the relationship between different factors and injury severity. The injury severity is divided into four categories. The analysis uses automobile and electric bicycle crash data of Xi’an, China, between 2014 and 2019. During the peak traffic periods, the impact of low visibility significantly varies with factors such as areas with traffic control or without streetlights. Furthermore, compared with traveling in a straight line, three different turnings before the crash reduce the likelihood of severe injuries. Roadside protection trees are the most crucial measure guaranteeing riders’ safety during peak traffic periods. This study reveals the direction, magnitude, and randomness of factors that contribute to electric bicycle crashes. The results can help safety authorities devise targeted transportation safety management and planning strategies for peak traffic periods.
Patient-Derived Human Basal and Cutaneous Squamous Cell Carcinoma Tissues Display Apoptosis and Immunomodulation following Gas Plasma Exposure with a Certified Argon Jet
Authors: Saadati Fariba , Moritz Juliane , Berner Julia , Freund Eric , Miebach Lea , Helfrich Iris , Stoffels Ingo , Emmert Steffen , Bekeschus Sander
Published: October 2021

Reactive oxygen species (ROS) have been subject of increasing interest in the pathophysiology and therapy of cancers in recent years. In skin cancer, ROS are involved in UV-induced tumorigenesis and its targeted treatment via, e.g., photodynamic therapy. Another recent technology for topical ROS generation is cold physical plasma, a partially ionized gas expelling dozens of reactive species onto its treatment target. Gas plasma technology is accredited for its wound-healing abilities in Europe, and current clinical evidence suggests that it may have beneficial effects against actinic keratosis. Since the concept of hormesis dictates that low ROS levels perform signaling functions, while high ROS levels cause damage, we investigated herein the antitumor activity of gas plasma in non-melanoma skin cancer. In vitro, gas plasma exposure diminished the metabolic activity, preferentially in squamous cell carcinoma cell (SCC) lines compared to non-malignant HaCaT cells. In patient-derived basal cell carcinoma (BCC) and SCC samples treated with gas plasma ex vivo, increased apoptosis was found in both cancer types. Moreover, the immunomodulatory actions of gas plasma treatment were found affecting, e.g., the expression of CD86 and the number of regulatory T-cells. The supernatants of these ex vivo cultured tumors were quantitatively screened for cytokines, chemokines, and growth factors, identifying CCL5 and GM-CSF, molecules associated with skin cancer metastasis, to be markedly decreased. These findings suggest gas plasma treatment to be an interesting future technology for non-melanoma skin cancer topical therapy.
In Vivo and In Vitro Evaluation of Urinary Biomarkers in Ischemia/Reperfusion-Induced Kidney Injury
Authors: Hosohata Keiko , Jin Denan , Takai Shinji
Published: October 2021

Oxidative stress plays an important role in the pathophysiology of acute kidney injury (AKI). Previously, we reported that vanin-1, which is involved in oxidative stress, is associated with renal tubular injury. This study was aimed to determine whether urinary vanin-1 is a biomarker for the early diagnosis of AKI in two experimental models: in vivo and in vitro. In a rat model of AKI, ischemic AKI was induced in uninephrectomized rats by clamping the left renal artery for 45 min and then reperfusing the kidney. On Day 1 after renal ischemia/reperfusion (I/R), serum creatinine (SCr) in I/R rats was higher than in sham-operated rats, but this did not reach significance. Urinary N-acetyl-β-D-glucosaminidase (NAG) exhibited a significant increase but decreased on Day 2 in I/R rats. In contrast, urinary vanin-1 significantly increased on Day 1 and remained at a significant high level on Day 2 in I/R rats. Renal vanin-1 protein decreased on Days 1 and 3. In line with these findings, immunofluorescence staining demonstrated that vanin-1 was attenuated in the renal proximal tubules of I/R rats. Our in vitro results confirmed that the supernatant from HK-2 cells under hypoxia/reoxygenation included significantly higher levels of vanin-1 as well as KIM-1 and NGAL. In conclusion, our results suggest that urinary vanin-1 might be a potential novel biomarker of AKI induced by I/R.
Epigenetic Dysregulations in Merkel Cell Polyomavirus-Driven Merkel Cell Carcinoma
Authors: Rotondo John Charles , Mazziotta Chiara , Lanzillotti Carmen , Tognon Mauro , Martini Fernanda
Published: October 2021

Merkel cell polyomavirus (MCPyV) is a small DNA virus with oncogenic potential. MCPyV is the causative agent of Merkel Cell Carcinoma (MCC), a rare but aggressive tumor of the skin. The role of epigenetic mechanisms, such as histone posttranslational modifications (HPTMs), DNA methylation, and microRNA (miRNA) regulation on MCPyV-driven MCC has recently been highlighted. In this review, we aim to describe and discuss the latest insights into HPTMs, DNA methylation, and miRNA regulation, as well as their regulative factors in the context of MCPyV-driven MCC, to provide an overview of current findings on how MCPyV is involved in the dysregulation of these epigenetic processes. The current state of the art is also described as far as potentially using epigenetic dysregulations and related factors as diagnostic and prognostic tools is concerned, in addition to targets for MCPyV-driven MCC therapy. Growing evidence suggests that the dysregulation of HPTMs, DNA methylation, and miRNA pathways plays a role in MCPyV-driven MCC etiopathogenesis, which, therefore, may potentially be clinically significant for this deadly tumor. A deeper understanding of these mechanisms and related factors may improve diagnosis, prognosis, and therapy for MCPyV-driven MCC.
The Burden of Peritoneal Metastases from Gastric Cancer: A Systematic Review on the Incidence, Risk Factors and Survival
Authors: Rijken Anouk , Lurvink Robin J. , Luyer Misha D. P. , Nieuwenhuijzen Grard A. P. , van Erning Felice N. , van Sandick Johanna W. , de Hingh Ignace H. J. T.
Published: October 2021

The peritoneum is a common metastatic site in gastric cancer. This systematic review provides an overview of the incidence, risk factors and survival of synchronous peritoneal metastases from gastric cancer. A systematic search was performed to identify studies wherein the incidence, risk factors and survival of gastric cancer with peritoneal metastases were investigated. Of all 38 potentially eligible studies, 17 studies were included based on the eligibility criteria. The incidence of synchronous gastric peritoneal metastases was reviewed for population-based studies (10–21%), for observational cohort studies (2–15%) and for surgical cohort studies (13–40%). Potential risk factors for synchronous gastric peritoneal metastases were younger age, non-cardia gastric cancer, female sex, signet ring cell carcinoma, diffuse type histology or linitis plastica, T4 stage, Hispanic ethnicity and more than one metastatic location. Synchronous peritoneal metastases are commonly diagnosed in patients with gastric cancer with an incidence up to 21% in recent population-based studies. Furthermore, prognosis of patients with gastric peritoneal metastases is poor with median overall survival ranging from 2 to 9 months. The high incidence and poor prognosis require intensive research on diagnostic features and effective treatment options to improve survival.
Pharmacological Treatments against COVID-19 in Pregnant Women
Authors: Arco-Torres Ana , Cortés-Martín Jonathan , Tovar-Gálvez María Isabel , Montiel-Troya María , Riquelme-Gallego Blanca , Rodríguez-Blanque Raquel
Published: October 2021

The recent respiratory virus known as SARS-CoV-2 has caused millions of deaths worldwide, causing great uncertainty due to the lack of a specific treatment, which has been mitigated by the use of various drugs traditionally used against other types of pathologies. Pregnancy presents special physiological conditions that expose the pregnant woman and the foetus to greater risk. Pregnant women are often excluded from trials due to possible risk of toxicity or side effects, resulting in a lack of knowledge about the use of drugs and treatments during pregnancy. The main objective of this review was to compile existing knowledge about currently available drug treatments for COVID-19 in pregnant women. The review report met the criteria of the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) review protocol and was registered with the registration number CRD42021251036. The electronic databases searched were Scopus, PubMed, CINAHL and SciELO. Finally, 22 articles were included, resulting in an analysis of drugs with an acceptable safety profile in the treatment of pregnant women with COVID-19.
Perceptions of Patients and Their Families Regarding Limitation of Therapeutic Effort in the Intensive Care Unit
Authors: Muñoz Camargo Juan Carlos , Hernández-Martínez Antonio , Rodríguez-Almagro Julián , Parra-Fernández María Laura , Prado-Laguna María del Carmen , Martín Mairena
Published: October 2021

Objective: Our objective was to determine and describe the opinion and attitudes of patients and their families regarding the limitation of therapeutic effort and advanced directives in critical patients and whether end-of-life planning occurs. Religious affiliation, education level, and pre-admission quality of life were also evaluated to determine whether they may influence decisions regarding appropriate therapeutic effort. Methods: A prospective, observational and descriptive study, approved by the center’s ethical committee, was carried out with 257 participants (94 patients and 163 family members) in the intensive care unit (ICU). A questionnaire regarding the opinions of patients and relatives about situations of therapeutic appropriateness in case of poor prognosis or poor quality of life was used. The questionnaire had three sections. In the first section, sociodemographic features were investigated. In the second section, information was collected on the quality of life and functional situation before ICU admission (taking as a reference the situation one month before admission) assessed by the Karnofsky scale, Barthel index, and the PAEEC scale (Project for the Epidemiological Analysis of Critical Care Patients). The third section aimed to determine whether the family knew the patient’s opinion regarding his/her end of life. Results: Of those interviewed, 62.2% would agree to limit treatment in case of poor prognosis or poor quality of future life. In contrast, 37.7% considered that they should fight for life, even if it is irretrievable. Only 1.6% had advanced directives registered, 43.9% of the participants admitted deterioration in their quality of life before ICU admission, 18.2% with moderate-severe deterioration. Our study shows that the higher the educational level, the lower the desire to fight for life when it is irretrievable and the greater the agreement to limit treatment. Besides, those participants not affiliated with a religion were significantly less likely to fight for life, including when irretrievable, than Catholics and were more likely to agree to limit treatment. Conclusions: More than half of the participants would agree to limit treatment in the case of a poor prognosis. Our results indicate that patients do not prepare for the dying process well in advance. Religion and educational level were determining factors for the choice of procedures at the end of life, both for patients and their families.
Dental Erosion in Obese Patients before and after Bariatric Surgery: A Cross-Sectional Study
Authors: Yang Cui , Hammer Frederik Johannes , Reissfelder Christoph , Otto Mirko , Vassilev Georgi
Published: October 2021

Obese patients are at risk of dental erosion due to micronutrient deficiency, consumption of soft drinks, gastric reflux disease and vomiting. The present study evaluates the presence of dental erosion in obese patients before and after bariatric surgery using the BEWE (basic erosive wear examination) scoring system. A total of 62 patients with severe obesity were included in the analysis, 31 in the control group (without bariatric surgery) and 31 in the surgery group (after bariatric surgery). BEWE scores did not vary between groups. Vitamin D deficiency was detected in 19 patients in the control group and three in the surgery group (p < 0.001). The serum calcium and vitamin D values were significantly higher in the surgery group (p = 0.003, p < 0.001 consecutively). All patients after bariatric surgery showed compliance with supplements, including vitamin D and calcium daily. Patients after bariatric surgery were less likely to drink soft drinks regularly (p = 0.026). Obese patients, before or after bariatric surgery, are at risk for erosive dental wear. However, with sufficient education prior to surgery and consistent intake of vitamin and mineral supplements, significant erosive dental wear after bariatric surgery could be avoided. Regular dental examination should be included in the check-up and follow-up program.
Impact of the Gestational Diabetes Diagnostic Criteria during the Pandemic: An Observational Study
Authors: Molina-Vega María , Gutiérrez-Repiso Carolina , Lima-Rubio Fuensanta , Suárez-Arana María , Linares-Pineda Teresa María , Cobos Díaz Andrés , Tinahones Francisco J. , Morcillo Sonsoles , Picón-César María J.
Published: October 2021

Objective: To analyze the effect of applying alternative diagnostic criteria for gestational diabetes mellitus (GDM) during the COVID-19 pandemic on GDM prevalence and obstetrical and perinatal outcomes, in comparison to usual diagnostic approaches. Methods: Data from women referred to GDM diagnosis from 1 September to 30 November 2019 were retrospectively collected (2019-group). The same data from the same period in 2020 were prospectively collected (2020-group). In both cases, a two-step diagnostic approach was used, the first step being a screening test (1 h 50 goral glucose tolerance test, OGTT). In 2019 it was followed by a 100 gr OGTT for diagnosis. In 2020, this was replaced by a blood test for the measurement of plasma glucose and HbA1c, according to alternative GDM diagnostic criteria during the COVID-19 pandemic. Results: From 237 women in the 2019 group, 40 (16.9%) were diagnosed with GDM, while from 255 women in the 2020 group, 37 (14.5%) had GDM (p = 0.470). More women in the 2020 group, in comparison to the 2019 group, were nulligravid (41.9% vs. 47.2%, p = 0.013), had a personal history of GDM (11.4% vs. 4.6%, p = 0.013) and had macrosomia in previous pregnancies (10.2% vs. 2.1%, p = 0.001). Obstetrical and perinatal outcomes were similar when comparing women with GDM to non-GDM women in the 2019 and 2020 groups and between GDM women and non-GDM women. Conclusion: In a Spanish population, GDM prevalence during the COVID-19 pandemic using the alternative diagnostic criteria was similar to that found in 2019 using the usual diagnostic criteria. Despite women referred for GDM diagnosis during the pandemic having more GDM risk factors, obstetrical and perinatal outcomes were comparable to those observed before the pandemic.
Establishment and Characterization of NCC-DDLPS4-C1: A Novel Patient-Derived Cell Line of Dedifferentiated Liposarcoma
Authors: Tsuchiya Ryuto , Yoshimatsu Yuki , Noguchi Rei , Sin Yooksil , Ono Takuya , Akiyama Taro , Hirose Takeshi , Iwata Shintaro , Yoshida Akihiko , Ohtori Seiji , Kawai Akira , Kondo Tadashi
Published: October 2021

Dedifferentiated liposarcoma (DDLPS) is a highly malignant sarcoma characterized by the co-amplification of MDM2 and CDK4. Although systemic chemotherapy is recommended for unresectable or metastatic cases, DDLPS is insensitive to conventional chemotherapy, leading to an unfavorable prognosis. Therefore, novel treatment methods are urgently required. Patient-derived cell lines are essential in preclinical studies. Recently, large-scale screening studies using a number of cell lines have been actively conducted for the development of new therapeutic drugs. However, the DDLPS cell line cannot be obtained from public cell banks owing to its rarity, hindering screening studies. As such, novel DDLPS cell lines need to be established. Accordingly, this study aimed to establish a novel DDLPS cell line from surgical specimens. The cell line was named NCC-DDLPS4-C1. NCC-DDLPS4-C1 cells retained copy number alterations corresponding to the original tumors. Further, the cells demonstrated constant growth, spheroid formation, and equivalent invasiveness to MG63 osteosarcoma cells. We also conducted drug screening and integrated the results with those of the previously reported DDLPS cell lines. Consequently, we identified the histone deacetylase inhibitor romidepsin as a novel candidate drug. In conclusion, the NCC-DDLPS4-C1 cell line is a useful tool for the basic study of DDLPS.
Personalized Reconstruction of Genital Defects in Complicated Wounds with Vertical Rectus Abdominis Myocutaneous Flaps including Urethral Neo-Orifice
Authors: Horch Raymund E. , Ludolph Ingo , Arkudas Andreas , Cai Aijia
Published: October 2021

Non-healing extensive wounds in the perineal region can lead to severe soft tissue infections and disastrous complications, which are not manageable with conservative measures. Specifically in recurrent or advanced pelvic malignancies, irradiation often leads to extensive scarring and wound breakdown, resulting in significant soft tissue defects during surgical tumor excision. Among several surgical options to reconstruct the perineum, the transpelvic vertical rectus abdominis myocutaneous (VRAM) flap has proven to be one of the most reliable methods. Specific modifications of this flap allow an individualized procedure depending on the patient’s needs. We modified this technique to include the urethral orifice into the skin paddle of VRAM flaps in three patients as a novel option to circumvent urinary diversion and maintain an acceptable quality of life.
Tomentosin a Sesquiterpene Lactone Induces Antiproliferative and Proapoptotic Effects in Human Burkitt Lymphoma by Deregulation of Anti- and Pro-Apoptotic Genes
Authors: Virdis Patrizia , Marchesi Irene , Fiorentino Francesco Paolo , Migheli Rossana , Sanna Luca , Bordoni Valentina , Pintore Giorgio , Galleri Grazia , Muroni Maria Rosaria , Bagella Luigi , Fozza Claudio , De Miglio Maria Rosaria , Podda Luigi
Published: October 2021

(1) Tomentosin is the most representative sesquiterpene lactone extracted by I. viscosa. Recently, it has gained particular attention in therapeutic oncologic fields due to its anti-tumor properties. (2) In this study, the potential anticancer features of tomentosin were evaluated on human Burkitt’s lymphoma (BL) cell line, treated with increasing tomentosin concentration for cytotoxicity screening. (3) Our data showed that both cell cycle arrest and cell apoptosis induction are responsible of the antiproliferative effects of tomentosin and may end in the inhibition of BL cell viability. Moreover, a microarray gene expression profile was performed to assess differentially expressed genes contributing to tomentosin activity. Seventy-five genes deregulated by tomentosin have been identified. Downregulated genes are enriched in immune-system pathways, and PI3K/AKT and JAK/STAT pathways which favor proliferation and growth processes. Importantly, different deregulated genes identified in tomentosin-treated BL cells are prevalent in molecular pathways known to lead to cellular death, specifically by apoptosis. Tomentosin-treatment in BL cells induces the downregulation of antiapoptotic genes such as BCL2A1 and CDKN1A and upregulation of the proapoptotic PMAIP1 gene. (4) Overall, our results suggest that tomentosin could be taken into consideration as a potential natural product with limited toxicity and relevant anti-tumoral activity in the therapeutic options available to BL patients.
Biological and Clinical Significance of Adaptive Evolution of Coronaviruses
Authors: Beloukas Apostolos , Rampias Theodoros
Published: October 2021

RNA viral genomes are generally small genomes that rarely exceed 10 kb in size [...]
PBPK Modeling and Simulation of Antibiotics Amikacin, Gentamicin, Tobramycin, and Vancomycin Used in Hospital Practice
Authors: Ferreira Abigail , Martins Helena , Oliveira José Carlos , Lapa Rui , Vale Nuno
Published: October 2021

The importance of closely observing patients receiving antibiotic therapy, performing therapeutic drug monitoring (TDM), and regularly adjusting dosing regimens has been extensively demonstrated. Additionally, antibiotic resistance is a contemporary concerningly dangerous issue. Optimizing the use of antibiotics is crucial to ensure treatment efficacy and prevent toxicity caused by overdosing, as well as to combat the prevalence and wide spread of resistant strains. Some antibiotics have been selected and reserved for the treatment of severe infections, including amikacin, gentamicin, tobramycin, and vancomycin. Critically ill patients often require long treatments, hospitalization, and require particular attention regarding TDM and dosing adjustments. As these antibiotics are eliminated by the kidneys, critical deterioration of renal function and toxic effects must be prevented. In this work, clinical data from a Portuguese cohort of 82 inpatients was analyzed and physiologically based pharmacokinetic (PBPK) modeling and simulation was used to study the influence of different therapeutic regimens and parameters as biological sex, body weight, and renal function on the biodistribution and pharmacokinetic (PK) profile of these four antibiotics. Renal function demonstrated the greatest impact on plasma concentration of these antibiotics, and vancomycin had the most considerable accumulation in plasma over time, particularly in patients with impaired renal function. Thus, through a PBPK study, it is possible to understand which pharmacokinetic parameters will have the greatest variation in a given population receiving antibiotic administrations in hospital context.
Role of PNPLA3 in the Assessment and Monitoring of Hepatic Steatosis and Fibrosis in Patients with Chronic Hepatitis C Infection Who Achieved a Sustained Virologic Response
Authors: Gavril Oana Irina , Arhire Lidia Iuliana , Gavrilescu Otilia , Dranga Mihaela , Barboi Oana , Gavril Radu Sebastian , Popescu Roxana , Cijevschi Prelipcean Cristina , Trifan Anca-Victorita , Mihai Catalina
Published: October 2021

Background and Objectives: Hepatic diseases are an important public health problem. All patients with chronic hepatitis C virus (HCV) infection receive treatment, regardless of hepatic fibrosis severity. However, evaluation of hepatic fibrosis and steatosis is still useful in assessing evolution, prognosis and monitoring of hepatic disease, especially after treatment with direct-acting antivirals (DAAs). The aim of this study was to assess the link between patatin-like phospholipase domain-containing 3 (PNPLA3) polymorphism and the degree of hepatic steatosis and fibrosis in patients with chronic HCV infection, as well as changes in steatosis and fibrosis three monthsafter obtaining a sustained viral response (SVR). Materials and Methods:Ourstudy included 100 patients with chronic hepatitis C (CHC) infection and compensated cirrhosis who received DAA treatment and who were evaluated using Fibromax prior to and 3 months after SVR. The influence of PNPLA3 (CC, CG, GG) genotype among these patients on the degree of post-treatment regression of steatosis and fibrosis was assessed. Results: Regression was noticed in the degree of both hepatic steatosis and hepatic fibrosis post-DAA treatment (three months after SVR). Analysis of the correlation between PNPLA3 genotype and fibrosis indicated that the average level of fibrosis (F) before DAA treatment was higher in patients with the GG genotype than in patients with the CC or CG genotype. Three months after SVR, the average level of fibrosis decreased; however, it remained significantly increased in GG subjects compared to that in CC or CG patients. The degree of hepatic steatosis before treatment was not significantly different among patients with different PNPLA3 genotypes, and no significant correlations were observed three months after SVR. Conclusions: The genetic variants of PNPLA3 influence the evolution of hepatic fibrosis. The GG subtype plays an important role in the degree of hepatic fibrosis both before and after treatment (three months after SVR)and could be a prognostic marker for assessment of post-SVR evolution.
A Magnetically Coupled Electromagnetic Energy Harvester with Low Operating Frequency for Human Body Kinetic Energy
Authors: Li Xiang , Meng Jinpeng , Yang Chongqiu , Zhang Huirong , Zhang Leian , Song Rujun
Published: October 2021

In this paper, a magnetically coupled electromagnetic energy harvester (MCEEH) is proposed for harvesting human body kinetic energy. The proposed MCEEH mainly consists of a pair of spring-connected magnets, coils, and a free-moving magnet. Specifically, the interaction force between the magnets is repulsive. The main feature of this structure is the use of a magnetic-spring structure to weaken the hardening response caused by the repulsive force. The magnetic coupling method enables the energy harvester system to harvest energy efficiently at low frequency. The MCEEH is experimentally investigated for improving energy harvesting efficiency. Under harmonic excitation with an acceleration of 0.5 g, the MCEEH reaches resonance frequency at 8.8 Hz and the maximum output power of the three coils are 5.2 mW, 2.8 mW, and 2.5 mW, respectively. In the case of hand-shaking excitation, the generator can obtain the maximum voltage of 0.6 V under the excitation acceleration of 0.2 g and the excitation frequency of 3.4 Hz. Additionally, a maximum instantaneous power can be obtained of about 26 mW from the human body’s kinetic energy.
Optimal Design for Compliant Mechanism Flexure Hinges: Bridge-Type
Authors: Wang Chia-Nan , Yang Fu-Chiang , Nguyen Van Thanh Tien , Nguyen Quoc Manh , Huynh Ngoc Thai , Huynh Thanh Thuong
Published: October 2021

Compliant mechanisms’ design aims to create a larger workspace and simple structural shapes because these mechanical systems usually have small dimensions, reduced friction, and less bending. From that request, we designed optimal bridge-type compliant mechanism flexure hinges with a high magnification ratio, low stress by using a flexure joint, and especially no friction and no bending. This joint was designed with optimal dimensions for the studied mechanism by using the method of grey relational analysis (GRA), which is based on the Taguchi method (TM), and finite element analysis (FEA). Grey relational grade (GRG) has been estimated by an artificial neural network (ANN). The optimal values were in good agreement with the predicted value of the Taguchi method and regression analysis. The finite element analysis, signal-to-noise analysis, surface plot, and analysis of variance demonstrated that the design dimensions significantly affected the equivalent stress and displacement. The optimal values of displacement were also verified by the experiment. The outcomes were in good agreement with a deviation lower than 6%. Specifically, the displacement amplification ratio was obtained as 65.36 times compared with initial design.
Progress and Perspectives in Designing Flexible Microsupercapacitors
Authors: Li La , Hu Chuqiao , Liu Weijia , Shen Guozhen
Published: October 2021

Miniaturized flexible microsupercapacitors (MSCs) that can be integrated into self-powered sensing systems, detecting networks, and implantable devices have shown great potential to perfect the stand-alone functional units owing to the robust security, continuously improved energy density, inherence high power density, and long service life. This review summarizes the recent progress made in the development of flexible MSCs and their application in integrated wearable electronics. To meet requirements for the scalable fabrication, minimization design, and easy integration of the flexible MSC, the typical assembled technologies consist of ink printing, photolithography, screen printing, laser etching, etc., are provided. Then the guidelines regarding the electrochemical performance improvement of the flexible MSC by materials design, devices construction, and electrolyte optimization are considered. The integrated prototypes of flexible MSC-powered systems, such as self-driven photodetection systems, wearable sweat monitoring units are also discussed. Finally, the future challenges and perspectives of flexible MSC are envisioned.
A Novel Multi-Ingredient Supplement Activates a Browning Program in White Adipose Tissue and Mitigates Weight Gain in High-Fat Diet-Fed Mice
Authors: Nederveen Joshua P. , Manta Katherine , Bujak Adam L. , Simone Alexander C. , Fuda Matthew R. , Nilsson Mats I. , Hettinga Bart P. , Hughes Meghan C. , Perry Christopher G. R. , Tarnopolsky Mark A.
Published: October 2021

We investigated the effects of a novel multi-ingredient supplement comprised of polyphenol antioxidants and compounds known to facilitate mitochondrial function and metabolic enhancement (ME) in a mouse model of obesity. In this study, 6-week-old male C57/BL6J mice were placed on a high-fat diet (HFD; ~60% fat) for 6 weeks, with subsequent allocation into experimentalgroups for 4 weeks: HFD control, HFD + ME10 (10 components), HFD + ME7 (7 components), HFD + ME10 + EX, HFD + EX (where ‘+EX’ animals exercised 3 days/week), and chow-fed control. After the intervention, HFD control animals had significantly greater body weight and fat mass. Despite the continuation of HFD, animals supplemented with multi-ingredient ME or who performed exercise training showed an attenuation of fat mass and preservation of lean body mass, which was further enhanced when combined (ME+EX). ME supplementation stimulated the upregulation of white and brown adipose tissue mRNA transcripts associated with mitochondrial biogenesis, browning, fatty acid transport, and fat metabolism. In WAT depots, this was mirrored by mitochodrial oxidative phosphorylation (OXPHOS) protein expression, and increased in vivo fat oxidation measured via CLAMS. ME supplementation also decreased systemic and local inflammation markers. Herein, we demonstrated that novel multi-ingredient nutritional supplements induced significant fat loss independent of physical activity while preserving muscle mass in obese mice. Mechanistically, these MEs appear to act by inducing a browning program in white adipose tissue and decreasing other pathophysiological impairments associated with obesity, including mitochondrial respiration alterations induced by HFD.
Assessment of Ceragenins in Prevention of Damage to Voice Prostheses Caused by Candida Biofilm Formation
Authors: Spałek Jakub , Daniluk Tamara , Godlewski Adrian , Deptuła Piotr , Wnorowska Urszula , Ziembicka Dominika , Cieśluk Mateusz , Fiedoruk Krzysztof , Ciborowski Michał , Krętowski Adam , Góźdź Stanisław , Durnaś Bonita , Savage Paul B. , Okła Sławomir , Bucki Robert
Published: October 2021

This study aimed to investigate the potential application of ceragenins (CSAs) as new candidacidal agents to prevent biofilm formation on voice prostheses (VPs). The deterioration of the silicone material of VPs is caused by biofilm growth on the device which leads to frequent replacement procedures and sometimes serious complications. A significant proportion of these failures is caused by Candida species. We found that CSAs have significant candidacidal activities in vitro (MIC; MFC; MBIC), and they effectively eradicate species of yeast responsible for VP failure. Additionally, in our in vitro experimental setting, when different Candida species were subjected to CSA-13 and CSA-131 during 25 passages, no tested Candida strain showed the significant development of resistance. Using liquid chromatography–mass spectrometry (LC-MS), we found that VP immersion in an ethanol solution containing CSA-131 results in silicon impregnation with CSA-131 molecules, and in vitro testing revealed that fungal biofilm formation on such VP surfaces was inhibited by embedded ceragenins. Future in vivo studies will validate the use of ceragenin-coated VP for improvement in the life quality and safety of patients after a total laryngectomy.
AIP1, Encoding the Small Subunit of Acetolactate Synthase, Is Partially Responsible for Resistance to Hypoxic Stress in Arabidopsis thaliana
Authors: Im Geunmuk , Choi Dongsu
Published: October 2021

Flooding is a significant stress to land plants, depriving them of essential oxygen. Plants have evolved diverse strategies with variable success to survive flooding. Similar strategies have been described in organisms from other kingdoms. Several fungal species can successfully survive a low-oxygen environment by increasing their branched-chain amino acid (BCAA) contents. BCAAs may act as alternative electron acceptors in the respiratory chain under an oxygen-limited environment. The key and first enzyme for BCAA biosynthesis is acetolactate synthase (ALS). We identified two homologous genes encoding the small subunit of ALS in Arabidopsis (Arabidopsis thaliana). We determined that ALS INTERACTING PROTEIN1 (AIP1), which encodes the small subunit of ALS, is strongly expressed in all organs and highly expressed under submergence and low-oxygen stresses. We also showed that the overexpression of AIP1 confers tolerance to low-oxygen stress. These results indicate that ALS may play an essential role under prolonged flooding or oxygen deficiency in Arabidopsis.
Use Patterns, Knowledge Diversity and Drivers for the Cultivation of the Miracle Plant [Synsepalum dulcificum (Schumach & Thonn.) Daniell] in Benin and Ghana
Authors: Tchokponhoué Dèdéou Apocalypse , N’Danikou Sognigbé , Fassinou Hotegni Nicodème Vodjo , Nyadanu Daniel , Kahane Rémi , Odindo Alfred Oduor , Achigan-Dako Enoch Gbènato , Sibiya Julia
Published: October 2021

Despite the growing interest in the miracle plant worldwide due to its numerous applications, the threats and the wild harvest of the species hamper its sustainable utilisation. Moreover, traditional knowledge so far documented on the species is limited to a narrow geographical coverage of its natural distribution range, which is West and Central Africa. This study analysed the use variation and knowledge acquisition pattern of the miracle plant among West African sociolinguistic groups and deciphered the drivers of populations’ willingness and readiness to engage in cultivating the species. Semi-structured interviews were conducted with 510 respondents purposively selected from nine sociolinguistic groups in Benin and Ghana using the snowball sampling approach. Information was collected on respondents’ socio-demographic profile, miracle plant ownership, plant parts used and preparation methods, knowledge of the species bioecology, perceived threats on the species, willingness to cultivate, maximum acreage to allocate to the species and maximum price to pay for a seedling. Descriptive statistics, generalized linear models, classification and regression tree models were used for data analysis. The miracle plant ownership mode depended on the age category. Sociolinguistic affiliation, level of schooling, migratory status and religion significantly affected the number of trees owned. We recorded 76 uses belonging to six use categories. The overall use-value of the miracle plant significantly varied according to the respondent sociolinguistic affiliation, main activity and religion. Men were the main source of knowledge and knowledge is mainly acquired along the family line. Knowledge related to food and social uses was mostly acquired from parents and people of the same generation, while magico-therapeutic and medicinal use-related knowledge were inherited from parents and grandparents. Sociolinguistic affiliation, awareness of taboos and market availability were the most important drivers of respondent willingness to cultivate the miracle plant. While the respondent’s level of schooling and perception of plant growth rate determined the maximum acreage they were willing to allocate to the species in cultivation schemes, their main activity, sociolinguistic affiliation and knowledge of the species time to fruiting drove the maximum purchase price they were willing to offer for a seedling of the species. Our findings provide key information for the promotion of miracle plant cultivation in the study area.
Transcriptomic Analysis Reveals Candidate Genes Responding Maize Gray Leaf Spot Caused by Cercospora zeina
Authors: He Wenzhu , Zhu Yonghui , Leng Yifeng , Yang Lin , Zhang Biao , Yang Junpin , Zhang Xiao , Lan Hai , Tang Haitao , Chen Jie , Gao Shibin , Tan Jun , Kang Jiwei , Deng Luchang , Li Yan , He Yuanyuan , Rong Tingzhao , Cao Moju
Published: October 2021

Gray leaf spot (GLS), caused by the fungal pathogen Cercospora zeina (C. zeina), is one of the most destructive soil-borne diseases in maize (Zea mays L.), and severely reduces maize production in Southwest China. However, the mechanism of resistance to GLS is not clear and few resistant alleles have been identified. Two maize inbred lines, which were shown to be resistant (R6) and susceptible (S8) to GLS, were injected by C. zeina spore suspensions. Transcriptome analysis was carried out with leaf tissue at 0, 6, 24, 144, and 240 h after inoculation. Compared with 0 h of inoculation, a total of 667 and 419 stable common differentially expressed genes (DEGs) were found in the resistant and susceptible lines across the four timepoints, respectively. The DEGs were usually enriched in ‘response to stimulus’ and ‘response to stress’ in GO term analysis, and ‘plant–pathogen interaction’, ‘MAPK signaling pathways’, and ‘plant hormone signal transduction’ pathways, which were related to maize’s response to GLS, were enriched in KEGG analysis. Weighted-Genes Co-expression Network Analysis (WGCNA) identified two modules, while twenty hub genes identified from these indicated that plant hormone signaling, calcium signaling pathways, and transcription factors played a central role in GLS sensing and response. Combing DEGs and QTL mapping, five genes were identified as the consensus genes for the resistance of GLS. Two genes, were both putative Leucine-rich repeat protein kinase family proteins, specifically expressed in R6. In summary, our results can provide resources for gene mining and exploring the mechanism of resistance to GLS in maize.
Light Deficiency Inhibits Growth by Affecting Photosynthesis Efficiency as well as JA and Ethylene Signaling in Endangered Plant Magnolia sinostellata
Authors: Lu Danying , Liu Bin , Ren Mingjie , Wu Chao , Ma Jingjing , Shen Yamei
Published: October 2021

The endangered plant Magnolia sinostellata largely grows in the understory of forest and suffers light deficiency stress. It is generally recognized that the interaction between plant development and growth environment is intricate; however, the underlying molecular regulatory pathways by which light deficiency induced growth inhibition remain obscure. To understand the physiological and molecular mechanisms of plant response to shading caused light deficiency, we performed photosynthesis efficiency analysis and comparative transcriptome analysis in M. sinostellata leaves, which were subjected to shading treatments of different durations. Most of the parameters relevant to the photosynthesis systems were altered as the result of light deficiency treatment, which was also confirmed by the transcriptome analysis. Gene Ontology and KEGG pathway enrichment analyses illustrated that most of differential expression genes (DEGs) were enriched in photosynthesis-related pathways. Light deficiency may have accelerated leaf abscission by impacting the photosynthesis efficiency and hormone signaling. Further, shading could repress the expression of stress responsive transcription factors and R-genes, which confer disease resistance. This study provides valuable insight into light deficiency-induced molecular regulatory pathways in M. sinostellata and offers a theoretical basis for conservation and cultivation improvements of Magnolia and other endangered woody plants.
Durability of Adult Plant Resistance Gene Yr18 in Partial Resistance Behavior of Wheat (Triticum aestivum) Genotypes with Different Degrees of Tolerance to Stripe Rust Disease, Caused by Puccinia striiformis f. sp. tritici: A Five-Year Study
Authors: Omar Ghady E. , Mazrou Yasser S. A. , EL-Kazzaz Mohammad K. , Ghoniem Kamal E. , Ashmawy Mammduh A. , Emeran Amero A. , Mabrouk Ola I. , Nehela Yasser
Published: October 2021

Adult plant resistance in wheat is an achievement of the breeding objective because of its durability in comparison with race-specific resistance. Partial resistance to wheat stripe rust disease was evaluated under greenhouse and field conditions during the period from 2016 to 2021. Misr 3, Sakha 95, and Giza 171 were the highest effective wheat genotypes against Puccinia striiformis f. sp. tritici races. Under greenhouse genotypes, Sakha 94, Giza 168, and Shandaweel1 were moderately susceptible, had the longest latent period and lowest values of the length of stripes and infection frequency at the adult stage. Partial resistance levels under field conditions were assessed, genotypes Sakha 94, Giza 168, and Shandaweel1 exhibited partial resistance against the disease. Leaf tip necrosis (LTN) was noted positively in three genotypes Sakha 94, Sakha 95, and Shandaweel1. Molecular analyses of Yr18 were performed for csLV34, cssfr1, and cssfr2 markers. Only Sakha 94 and Shandaweel1 proved to carry the Yr18 resistance allele at both phenotypic and genotypic levels. Scanning electron microscopy (SEM) observed that the susceptible genotypes were colonized extensively on leaves, but on the slow-rusting genotype, the pustules were much less in number, diminutive, and poorly sporulation, which is similar to the pustule of NIL Jupateco73 ‘R’.
Comparative Analysis of the Effect of Carbon- and Titanium-Ions Irradiation on Morpho-Anatomical and Biochemical Traits of Dolichos melanophthalmus DC. Seedlings Aimed to Space Exploration
Authors: De Micco Veronica , De Francesco Sara , Amitrano Chiara , Arena Carmen
Published: October 2021

The realization of manned missions for space exploration requires the development of Bioregenerative Life Support Systems (BLSSs) to make human colonies self-sufficient in terms of resources. Indeed, in these systems, plants contribute to resource regeneration and food production. However, the cultivation of plants in space is influenced by ionizing radiation which can have positive, null, or negative effects on plant growth depending on intrinsic and environmental/cultivation factors. The aim of this study was to analyze the effect of high-LET (Linear Energy Transfer) ionizing radiation on seed germination and seedling development in eye bean. Dry seeds of Dolichos melanophthalmus DC. (eye bean) were irradiated with two doses (1 and 10 Gy) of C- and Ti-ions. Seedlings from irradiated seeds were compared with non-irradiated controls in terms of morpho-anatomical and biochemical traits. Results showed that the responses of eye bean plants to radiation are dose-specific and dependent on the type of ion. The information obtained from this study will be useful for evaluating the radio-resistance of eye bean seedlings, for their possible cultivation and utilization as food supplement in space environments.