bluuebunny/crossref_metadata_2025 huggingface dataset:
Association of Urinary Biomarkers Noninvasively Forecasts The Extent of Renal Injury In The Early Diabetic Nephropathy Patients With Kidney Qi Deficiency Syndrome: A Retrospective Investigation 
Authors: Wu Wei , Meng Xian-Jie , Liu Ying-Lu , Wang Wen-Wen , Fang Qi-Jun , Yee Hong-Yun , Yuan Can-Can , Wang Mei-Zi , Chong Fee-Lan , Wan Zi-Yue , Wan Yi-Gang , Shen Shan-Mei
Published: October 2020

Background: Recently, diabetic nephropathy (DN) becomes a common health problem in China as one of the major microvascular complications of diabetes mellitus. Therefore, the incipient diagnosis and noninvasive detections in clinic by the association of urinary biomarkers are important for preventing the progress of DN. However, the clinical significance of urinary biomarkers is controversial nowadays. This study thereby aimed to further evaluate the clinical significance of the association of urinary biomarkers in noninvasively predicting the renal damaged extent of the early type 2 DN patients with kidney qi deficiency syndrome in an integrated traditional and western medical center, and preliminarily confirm the correlation between urinary tubular biomarkers and the biological bases of DN patients.Methods: Ninety-two patients in an integrated traditional and western medical center of China were categorized into 3 groups, 20 patients with normo-albuminuria, 50 patients with micro-albuminuria, and 22 patients with macro-albuminuria. In addition to urinary albumin (UAlb) and urinary albumin-to-creatinine ratio (UACR), serum creatinine, estimated glomerular filtration rate and various urinary tubular biomarkers were tested respectively. Besides, clinical characteristics and kidney asthenia syndrome distribution in all patients were observed. Results: In these 3 groups, 24-h UAlb and UACR showed stepwise and significant increases. Urinary cystatin C (UCysC), urinary N-acety1-β-D-glucosaminidase (UNAG) and urinary retinal binding protein (URBP) synchronously showed gradual increases consistent with albuminuria degree in 3 groups. Moreover, 24-h UAlb and UACR were positively correlated with UCysC, UNAG and URBP. In the 72 DN patients with albuminuria, there was a positive correlation between UNAG and URBP, and UCysC was also positively correlated with UNAG and URBP. Additionally, TCM syndrome distributional characteristics in all patients were consistent with the clinical manifestations of kidney qi deficiency syndrome. Conclusion: In this investigation, we ulteriorly demonstrated that the association within UCysC, UNAG, URBP and UAlb may be used as practical targets in noninvasively forecasting the extent of renal injury in the early type 2 DN patients with kidney qi deficiency syndrome. More importantly, we found that urinary tubular biomarkers may be one of the biological bases of DN patients with the specific traditional Chinese medicine syndrome.
Correlations Between Traditional Chinese Medicine Syndromes and Clinical Indexes in Health Check-Up Population
Authors: Liao Pei-Hung , Tu Chia-Ling , Liu Chi-Feng
Published: October 2020

BackgroundRoutine health check-up is associated with improved lifespan and reduced medical cost. Traditional Chinese medicine (TCM) serves as a cost-effective modality in healthcare system. We examined the correlations of TCM syndromes with modern medical indicators in health check-up population.MethodsWe studied 5231 subjects undergoing health check-up between January 1st 2008 and December 31, 2016. Physical indexes such as body weight and blood pressure and biomedical indicators like live function and tumour markers were measured. All subjects underwent colonoscopy. All subjects were classified and differentiated into five different TCM syndromes. An artificial neural network (ANN) was employed to evaluate the predictive value of TCM syndrome differentiation.ResultsOf enrolled subjects, SADH accounted for 85.8% and IDSIBSB was found in 4576 subject (87.5%). YaDSK and YiDLK accounted for 99.5% (5207) and 80.9% (4232) respectively. We found that YiDLK is correlated with abnormality of liver function indexes. The results showed that SADH is correlated with level of cholesterol in health check-up population. The results showed that the predictive ANN model showed a good fitting with an accuracy of 100%.ConclusionThe results demonstrated that TCM syndromes were closely correlated with clinical laboratory indexes regardless of health status. TCM syndrome differentiation is suggested to contribute to routine health examination as screening measure with its non-invasive nature.
Berbamine Inhibits Cell Proliferation, Migration, and Invasion and Induces Cell Apoptosis of A549 Cells Via Regulating C-Maf, PI3K/Akt and MDM2-P53 Pathways
Authors: Liu Lili , Xu Zhiying , Yu Binbin , Tao Li , Cao Ying
Published: October 2020

Background To investigate the influences of berbamine (BBM) on the cell viability, proliferation, and migration of A549 cells in vitro and in vivo, and explore the possible mechanisms.MethodsAfter the A549 cells were treated with BBM, the cell viability and proliferation of the cancer cells were detected by MTT assay, EdU assay, and colony formation assay. Migration and invasion of cancer cells were illustrated through wound scratch assay and transwell assay. Apoptosis of cancer cells was evaluated by trypan blue dye exclusion assay and elisa assay. Beside, the expression of PI3K/Akt signal pathway-related proteins and c-Maf were detected employing western blotting assay. Xenografted model of NSCLC was used to detect the effect of BBM on tumor growth and metastasis in vivo.ResultsMTT assay showed that BBM inhibited the viability of A549 cells in a concentration-dependent manner and time-dependent manner. The results from the colony formation assay and EdU assay revealed that BBM (10 µM) could significantly inhibit the proliferation of A549 cells (P＜0.001). And BBM (10 µM) significantly inhibited the migration and invasion in the wound scratch assay and transwell assay (P＜0.05). Trypan blue assay and elisa assay indicated that BBM (20 µM) significantly induced apoptosis of A549 cells. The nude mice assay manifested the tumor volume was significantly shrank by BBM (20 mg/kg) (P＜0.05). Western blotting assay showed that the PI3K/Akt and MDM2-p53 signaling pathways were inhibited by BBM, and the expression of c-Maf was downregulated by BBM. ConclusionsBBM could inhibit the proliferation and metastasis, and induce apoptosis of A549 cells in vitro and in vivo, these effects may be achieved by reducing the expression of c-Maf and regulating the PI3K/Akt and MDM2-p53 pathways.
First Systematic Analysis for Chemical Composition by HPLC-ESI-MSn and Antioxidant Activity Comparison of Dendrobium huoshanense and Dendrobium officinale
Authors: Wan Jingqiong , Gong Xiaohui , Wen Chongwei , Wei Yuan , Kong Lingdong , Han Bangxing , Ouyang Zhen
Published: October 2020

Background: Dendrobium huoshanense C. Z. Tang et S. J. Cheng (D. huoshanense) is a precious Dao-di herb with high quality, excellent efficacy and edible value of China, Dendrobium officinale Kimura et Migo (D. officinale) is widely utilized and commonly used as a substitute for D. huoshanense. But few studies have been conducted to systematically analyze chemical composition and bioactivities of the two.Methods: HPLC-ESI-MSn was used to identify the compounds in D. huoshanense and D. officinale grown in Huoshan from methanol extracts and different polar fractions of water extracts, including petroleum ether, ethyl acetate and n-butanol; The in vitro antioxidant activity were evaluated by the scavenging effects on DPPH and ·OH radicals, total reduction ability and total antioxidant ability.Results: 61 compounds were identified in D. huoshanense and 49 compounds were identified in D. officinale and with 43 shared compounds; Among them, 44 and 7 compounds were respectively identified from the two for the first time. Oxyresveratrol, quercetin-3-O-glucoside, quercetin-3-O-arabinoside, naringenin-7-O-glucoside, baicalin, hesperetin, vitexin and apigenin-6-C-pentosyl-8-C-(2’’-O-quinoyl)-glucoside were first identified in Dendrobium. In addition, 5 compounds were unique in D. huoshanense compared with other species of Dendrobium . The results of in vitro antioxidant activity showed that n-BuOH-soluble fraction of D. huoshanense and D. officinale both revealed the strongest antioxidant activities compared with other fractions, furthermore, the antioxidant activities of different fractions of D. huoshanense were separately superior to that of D. officinale.Conclusion: These results may provide a reference for the substances study about D. huoshanense's high-quality efficacy, and also provide a scientific basis for its development and utilization.
Exploring the Modulatory Mechanism of Eleng Capsule in the Treatment of Endometriosis Based on Integrated Strategy of Transcriptomics Combined With Network Pharmacology
Authors: Zheng Weilin , Wang Jie , Gu Jiangyong , Wu Jiayi , Wang Tao , Huang Yangxue , Liang Xuefang , Cao Lixing
Published: October 2020

BackgroundEndometriosis causes severe chronic pelvic pain and infertility. Chinese medicine plays an active role in the treatment of endometriosis. ELeng Capsule(ELC) is a Chinese medicine formula used for the treatment of endometriosis for several years. The previous studies have shown that ELC inhibits endometriosis. However, the mechanisms of action of ELC have not been characterized. In this study, network pharmacology and mRNA transcriptome analysis were used to study various therapeutic targets in ELC.Methods Ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry analysis(UPLC-Q-TOF/MS) was used to identify the compounds in ELC.And network pharmacology was used to analyze the network of targets and identified compounds of ELC. Apoptosis was assessed using the terminal deoxynucleotidyl transferase (TdT) deoxyuridine 5'-triphosphate (dUTP) nick-end labeling (TUNEL) assay.The protein expression of VEGFA,VEGFB,VEGFC and α-SMA in the ectopic endometrium were identified by immunohistochemistry(IHC).The level of VEGFA,VEGFB and IL1-β in serum were used by ELISA. Further, RNA-sequencing was used to identify differentially expressed genes (DEGs) in ELC. Biological functions and pathways were determined through the Gene Ontology(GO) and Kyoto encyclopedia of genes and genomes pathway(KEGG) analyses. In addition, Gene Set Enrichment Analysis (GSEA) analysis was used to further analyze the genetic network and modular genetics. ResultsWe had identified 26 new bioactive compounds in ELeng Capsule by UPLC-Q-TOF/MS analysis. The KEGG pathways of ELC associtaed targets related to neuroactive ligand-receptor interaction, the toll-like receptor signaling pathway, and the vascular endothelial growth factor signaling pathway by network pharmacology analysis.Further, ELC could induce cell apoptosis, inhibit angiogenesis through reduce the expression of VEGFA and VEGFC, and inhibit the fibrosis through reduce the expression of α-SMA in ectopic lesions(P＜0.05). In addition, the development of endometriosis in the rat model may be related to mechanisms of inflammation,epithelial-mesenchymal transition(EMT) by RNA-sequencing. And the targets in the treatment of ELC were predominantly associated with actin and cytoskeleton,EMT,focal adhesion,and inflammatory immunity based on the DEGs analysis. In additon, the GSEA analysis showed that the treatment of ELC was associated with the Notch signaling pathway, Hippo signaling pathway, etc.ConclusionELeng Capsules exerted beneficial effects against endometriosis, potentially by induce apoptosis,modulating the angiogenesis,cytoskeleton, epithelial-mesenchymal transition(EMT), and cell junction-associated pathways,etc. These findings could provide evidence for an innovative treatment strategy and novel therapeutic targets for endometriosis.
ShenLing BaiZhu San alleviates Ulcerative Colitis in Rats by Regulating Gut Microbiota
Authors: Gu Daxing , Zhou Shanshan , Yao Lili , Tan Ying , Chi Xingzi , Shi Dayou , Guo Shining , Liu Cui
Published: October 2020

Background: Recent studies have suggested that Shenling Baizhu San (SLBZS), an complementary and alternative medical therapy for ulcerative colitis (UC), alleviate clinic symptoms by the improvement of biochemical criteria and restoration of the intestinal barrier function. SLBZS as a famous Chinese herbal formula has been reportedly used to treat UC, of which mechanism is unknown. This study investigated the therapeutic effects of SLBZS on restoring the gut microbiota in a UC rat model. Methods: We proposed a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced UC rat model to monitor the structural modulation of the gut microbiota. The test period was 10 days (observation for two days after modeling, treatment for 8 days by SLBZS). In this study, the level of inflammatory cytokines and activity of antioxidant enzymes in serum were ascertained by enzyme-linked immunosorbent assay (ELISA) and histological changes of colon were observed. Feces were collected for high-throughput sequencing of 16S rRNA gene. Results: SLBZS partly reduced the diversity of the gut microbiota, while the abundance of that is increased. Furthermore, at the genus level, the relative abundance of short chain fatty acids (SCFA) producing bacteria including Prevotella and Oscillospira increased, while the relative abundance of harmful bacteria including Desulfovibrio, and Bilophila decreased. Additionally, SLBZS could improve the lesions of colon and significantly reduce the expression of Interleukin-6 (IL-6) and Myeloperoxidase (MPO) and increase the activities of Superoxide dismutase (SOD) and Catalase (CAT) in rats serum. Conclusions: These results demonstrate that SLBZS may treat UC effectively by inhibiting inflammation, enhancing antioxidant capacity and regulating gut microbiota.
Exploring the Pharmacological Mechanism of Cassiae Semen a Cting on Cataracts Based on a Network Pharmacology Approach
Authors: Zhong Ying , Fang Youfa
Published: October 2020

BackgroundCassiae Semen (CS) is one of the most well-known herbs used in the treatment of cataracts in China. However, the potential mechanisms of its anti-cataracts effects have not been fully explored.MethodThe active compounds of CS were obtained from TCMSP database, and their targets were retrieved from the TCMSP, STITCH and DrugBank databases. Cataracts related target genes were identified from the GeneCard, Malacard, and OMIM databases. GO and KEGG analysis were performed using DAVID online tools, and Cytoscape were used to construct compound-targets network and protein-protein interaction (PPI) networks, cluster analysis were carried out using MCODE plugin for Cytoscape.ResultsWe obtained 13 active compounds from CS and 105 targets in total to construct a compound-target network, which indicated that emodin, stigmastero, and rhein served as the main ingredients in CS. A total of 238 cataracts related targets were identified from public databases. PPI networks of compound targets and cataract-related targets were constructed and merged to obtained the central network, enrichment analysis showed 50 key targets in the central network enriched in several important signaling pathways, such as thyroid hormone signaling pathway, MAPK signaling pathway, PI3K-Akt signaling pathway. The top 4 genes with higher degree in the central network were TP53, HSP90, ESR1, EGFR, indicating their important roles in the treatment of cataracts.ConclusionsThe present study systematically revealed the multi-target mechanisms of CS on cataracts using network pharmacology approach, and provided indications for further mechanistic studies and also for the development of CS as a potential treatment for cataracts patients.
Synthesis, Structural and Dielectric Studies on SrBi2-xGdxNb2O9 Lead Free Ceramics
Authors: Jaladi Nitchal Kiran , Rao K. Sambasiva , Workineh Haileeyesus , Kiran J. Anindhya , Nagamani S.
Published: October 2020

In this manuscript, the structural and dielectric properties of Gadolinium (Gd3+) substituted at Bi-site of SrBi2-xGdxNb2O9 (x= 0.0, 0.4, 0.6 and 0.8) prepared by using solid state reaction are studied. XRD analysis revealed the formation of single phase with orthorhombic structure in SBN and Gadolinium modified SBN. It is found that cell parameters and volume were decreased with increase of Gd3+ ion concentration in SBN. SEM analysis revealed that the samples possess well defined needle shaped grains. The grain size of SBN was hindered by the presence of Gd3+ ion at Bi-site. The growth of single phase layered perovskite structure was confirmed from FTIR and Raman spectroscopy. The dielectric properties of Gd3+ ion doped SBN ceramics are studied as a function of frequency (50Hz-1MHz) from room temperature to 500ºC. It is observed that phase transition temperature (Tc) decreased from 430ºC to 330ºC with increase of frequency due to incorporation of Gd3+ ion in SBN. The broadness of peaks and decrease in Tc indicate the transition from a normal ferroelectric to ferroelectric-relaxor type. The study on variation of tanδ with temperature at different frequencies indicates that tanδ has larger values at higher temperatures. Further, the diffuseness parameter (γ) has been computed for all the compositions.
Characteristics of confirmed coronavirus disease cases at the south campus of Shanghai East Hospital: A retrospective study
Authors: Fan Chenghui , Qu Lihong , Guo Zhongliang , Zhang Jianhua , Wang Jun , Li Qin , Kang Sheng , Wang Yue , Xu Lei , Wang Wei , Yang Wenzhong , Zhou Ying , Wang Yuanyuan , Hao Ying , Li Ruilin , Sun Feng , Xue Junli
Published: October 2020

Background: A fever outpatient clinic at the south campus of Shanghai East Hospital (SEH) openedin response to the coronavirus disease (COVID-19) outbreak. We analyzed the data of all 11,972patients who visited the fever clinic and the 29 confirmed COVID-19 cases to determine the clinical and epidemiological characteristics of confirmed COVID-19 cases diagnosed at SEH.Methods: Data were collected from all fever outpatient clinic patients between January 23 and September 30, 2020. We compared the characteristics of confirmed patients, including age, occupation, area, symptoms, laboratory results, and computed tomography (CT) findings, according to month.Results: By September 30, 2020, 11,972 patients, including 29 (0.24%) confirmed COVID-19 cases, visited the clinic. Four of five confirmed domestic cases identified during January–February 2020 were from Wuhan (mainly elderly retirees and local employees), Hubei. After the epidemic spread internationally, all 22 confirmed cases identified during March–April 2020 were individuals who returned from abroad. They were predominantly young Chinese international students. The sporadic two confirmed cases during May–September 2020 included an employee returning to work from Hubei and an Indian servant from abroad. Symptoms, laboratory tests, and CT findings were consistent with previous reports of COVID-19-positive cases.Conclusions: The characteristics of confirmed COVID-19 cases at SEH varied among different periods in response to the spread of the pandemic. However, due to the effective early isolation and quarantine measures, no outbreak occurred in SEH, which contributed to the prevention and control of the epidemic in Shanghai.
Effect of Levosimendan on the Prognosis of Cardiac Surgery in Patients with Cardiac Insufficiency
Authors: Na Heya , Fa Xianen , Zhou Yuyang , Li Xiangyang , Zhu Rui , Liu Lei , Liu Bing
Published: October 2020

Objective: To retrospectively analyze the effect of levosimendan on the survival and prognosis of cardiac surgery patients with LVEF < 40%. Methods: the clinical data of 224 patients with preoperative LVEF < 40% were retrospectively analyzed. According to different treatment schemes, the patients were divided into levosimendan group (n = 60) and no-levosimendan group (n = 164,). The control group was treated with routine treatment, and the observation group was treated with levosimendan on the basis of routine treatment. Then a multivariate logistic regression model with a propensity score analysis was used to limit biases and finally the data of 40 patients in each group were selected for analysis. Results: Hemodynamic data showed that the cardiac index, LVEF and PAOP of patients in levosimendan group were significantly improved. The concentration of serum lactic acid in the levosimendan group was lower than that in the control group (P < 0.05). At the same time, postoperative ICU and hospital stay were significantly reduced in levosimendan group (P < 0.05.), Logistics regression analysis showed that levosimendan was the only protective factor for Low cardiac output syndrome (LCOS) (HR=4.33; 95% confidence interval: 1.27- 14.78; P = 0.019). Conclusion: levosimendan can better improve hemodynamics and reduce postoperative ICU time and hospital stay. The use of it tended to decrease the incidence of LCOS significantly.
The Clinical Characteristics and Treatment of Mesh Infection After Laparoscopic Inguinal Hernia Repair
Authors: Luo Yun , Wang Chongshu
Published: October 2020

Background: Mesh infection after inguinal hernia repair (MILIHR) is one of the rare and most serious complications. The aim of our study was to analyze the clinical characteristics and treatment methods of MILIHR.Methods: Two MILIHR cases admitted to Beibei Traditional Chinese Medical Hospital were reported, and 32 MILIHR cases reported in Chinese and English literatures were retrieved. The clinical features and treatment of MILIHR in 34 cases were analyzed.Results: In case 1, medical glue was found in in the infected area, and in case 2, the infection focal point was the residual hernia sac covering the pus. Risk factors of other cases included obesity, smoking, malignant tumor, diabetes, postoperative hematoma, postoperative aspiration of seroma, steroid or immunosuppressive drugs use, etc. The results of pathogen culture showed that there were 14 cases of Staphylococcus, 5 cases of Escherichia coli, 5 cases of Pseudomonas, 1 case of Hemolytic streptococcus, 2 cases of fungi, 2 cases of pseudoinfection (rejection). 33 cases were cured after the mesh was removed, and 1 case was cured after conservative treatment. The mesh was removed by laparoscopic surgery in 22 cases and by open surgery in 11 cases.Conclusions: The risk factors of MILIHR are multifactorial，and the using of medical glue and residual hernia sac may be the risk factors of mesh infection. The main pathogens of MILIHR were Staphylococcus, Escherichia coli and Pseudomonas. The most reliable way to treat MILIHR is to remove the mesh completely. The removal of the infected mesh by laparoscopic surgery can clearly identify the important anatomical structures and avoid anterior approach abdominal wall damage during re-hernia repair.
Cross-Sectional Surveys as A Tool To Study Epidemiological Factors And Exploring Social Dynamics of Seasonal Influenza In Malta
Authors: Marmarà Vincent , Marmarà Danika , McMenemy Paul , Kleczkowski Adam
Published: October 2020

Background: Seasonal influenza has major implications for healthcare services as outbreaks often lead to high activity levels in the health system. Mathematical models have extensively been used to predict epidemics of infectious diseases such as seasonal influenza and to assess effectiveness of proposed control strategies. However, the availability of comprehensive and reliable datasets is limited. In this paper we combine a unique epidemiological dataset collected in Malta through GPs with a novel method that uses cross-sectional surveys to study the dynamics of seasonal influenza in Malta in 2014-2016, to include social dynamics and self-perception related to seasonal influenza.Methods: Two cross-sectional public surveys (n=406 per survey) were performed by phone across the Maltese population in 2014-15 and 2015-16 influenza seasons. Survey results were compared with incidence data (diagnosed seasonal influenza cases) collected by GPs in the same period and with Google Trends data for Malta. The information was collected on whether participants recollected their health status in past months, occurrences of influenza symptoms, hospitalisation rates due to seasonal influenza, whether they sought GP advice, and other medical information. Results: We demonstrate that cross-sectional surveys are a reliable alternative source of data to medical records. The two surveys gave comparable results, indicating that the level of recollection among the public is high. Based on two seasons of data, the reporting rate in Malta varies between 14% and 22%. The comparison with Google Trends suggests that the online searches peak at about the same time as the maximum extent of the epidemic, but then the public interest declines and returns to the background level. We also found that the public intensively searched the Internet for influenza-related terms even when the number of cases was low.Conclusions: Our research shows that a telephone survey is an appropriate way to gain deeper insight into the population self-perception of influenza and its symptoms and to provide another benchmark for medical statistics provided by GPs and Google Trends. The information collected in different ways can be used to improve epidemiological modelling capacity for seasonal influenza and other infectious diseases, thus contributing to public health.
Ad﻿hatoda Vasica: a potential ayurvedic intervention against COVID-19 associated impaired immune response and hypoxia-inflammation phenotype
Authors: Gheware Atish , Dholakia Dhwani , Kannan S. , Panda Lipsa , Anand Vivek , Rani Ritu , Pattnaik Bijay Ranjan , Jain Vaibhav , Subramanian V , Mukerji Mitali , Agrawal Anurag , Prasher Bhavana
Published: October 2020

Background: The importance of hypoxia inducible factor-1 α (HIF-1α) stabilization in uncontrolled infection and inflammation is widely accepted. Several inhibitors of HIF signalling are in clinical trials for malignancy, ischemia and inflammatory diseases. Increased hypoxia is being reported to be an important modifier for several pathological features of COVID-19 such as impaired immunity, hyper-inflammation, thrombosis, lung injury and sepsis. Methods: In this study we tested the effect of whole aqueous extract Adhatoda Vasica (AV), that our group has shown to have anti-hypoxic and anti-inflammatory effects, on various outcomes of hypoxic response. Effects of AV were assessed in preclinical mouse models of pulmonary fibrosis, bacterial sepsis and siRNA induced hypoxia-thrombosis phenotype. Therapeutic relevance of AV in current pandemic were also examined through transcriptome and molecular docking analysis. Results: Oral administration AV extract attenuated the increased levels of airway inflammation, collagen content, transforming growth factor-β1 (TGF-β1), IL-6, HIF-1α and improved the overall survival rate in bleomycin treated and Cecum Ligation and Puncture (CLP) induced mice. AV treatment also rescued the prolyl hydroxylase domain 2 (phd2) siRNA induced HIF-1α and associated blood coagulation phenotypes in mice. Transcriptome analysis of lungs of AV treated naïve mice reveal downregulation of hypoxia, inflammation, TGF-β1 and angiogenesis and upregulation of adaptive immunity related genes. These genes and pathways show opposite expression in transcriptome of BALF and PBMCs of SARS-CoV2 infected patient. Molecular docking of AV constituents presents in extract reveal many molecules with low binding energy (≤ -8) to multiple SARS-CoV2 and host target proteins that are relevant in viral entry and replication. Conclusion: Our results provide a scientific rationale for this ayurvedic herbal medicine in ameliorating the anti-inflammatory and anti-HIF-1α effect for potential use in management of COVID19 patients.
ASSESSMENT OF STIGMATIZATION AND DISCRIMINATION OF HIV-INFECTED PERSONS IN PROFESSIONAL MEDICAL EDUCATION INSTITUTIONS
Authors: Datsyuk Victoriya
Published: October 2020

The aim of the study. Finding out the attitude of the respondents to HIV-infected / AIDS patients, their awareness of the ways of HIV infection.

Methods. A survey of teachers and students of Kyiv City Medical College was conducted (educational institution of 1–2 levels of accreditation). The total number of students, who participated in the study, was 150 people aged 17 to 25 years. The survey involved 62 teachers aged 30 to 65 years. In order to find out the level of stigmatization of people living with HIV, a questionnaire has been developed that contains questions, related to various aspects of HIV infection and the lives of HIV-infected people.

Results. The study showed a high willingness to stigmatize and discriminate against people living with HIV / AIDS by teachers and medical school workers. The problem of reasonable stigma and discrimination against people living with HIV is the low level of knowledge about the epidemic. This is especially true for work. A tolerant attitude towards HIV-infected people from work and lectures at medical college is directly related to the awareness of these problems.

The high level of stigma and discrimination against HIV-infected students and teachers in medical schools is reflected in the willingness to refuse medical care to people living with HIV. The state's information policy in the field of combating stigma and discrimination against HIV-infected people is imperfect and insufficient.

Сonclusions. The study shows the imperfection of the existing system of medical education in the field of upbringing of tolerant attitude to vulnerable groups, in particular to HIV-infected. In this area, the existing system of medical education needs to be improved and modernized. The aggravation of the problem of stigmatization and discrimination of HIV-infected people, the ineffectiveness of outdated methods requires the search for new approaches to its solution.
POPULAR MUSICAL TELEVISION PROJECTS OF THE LATE XX – EARLY XXI CENTURIES
Authors: Loktionova-Oitsius Oleksandra
Published: October 2020

The article is devoted to the study of the general state of television in the field of music performance. The most popular musical television programs and vocal shows are considered. The purpose of the work is to identify the features of music TV projects, comparing with world models and highlighting the typical features of the musical television space. The research methodology consists in applying the general principles of scientific knowledge that correspond to modern cultural discourse. The formation of musical and television projects in the context of changes in the social mentality of the consumer of mass culture is considered. Television is interpreted as a means of approaching the global process closer to a person, that is, a consumer communicates with world-wide examples of popular art, music television projects, vocal show projects and etc. It creates the preconditions for imitation of the best world models of music TV projects in Ukraine. The article first analyzes the interconnection of Ukrainian music television projects as analogues to such worldwide shows as "The Voice", "The X factor", "American Idol". Vocal talent shows are considered as combining the elements of a â€œgame showâ€ and a â€œperfection / transformation showâ€, promoting the development of the educational component, namely, the formation of educational activity through comments and advice of judges, classes with participants between performances, determination of the most successful performances, concert practice and etc. It was determined, that the vocal repertoire consists of the most popular world and national hits, which reflects the demand of the audience. It was noted, that the viewer influences the selection of participants in a music television project and functions as an additional judge. So, the article focuses on the structure and content of musical television projects, defines the values of such projects as the communication space of culture between the audience, the artistic and performing component, and the national and world music culture as a whole. Music television projects are part of the general educational context for the development of media art and have a scientific, artistic and educational potential for study.
The Destructive Effect of Economic Crime on the Process of Economy Socialization
Authors: Egoryshev Sergey
Published: October 2020

Over the past two decades, the problems of the economy socialization have gained increasing interest of Russian scientists. Economy socialization is 1) a civilizational regularity of socio-economic development, 2) a key component of the world economic transformation, 3) an essential condition for the formation of a social economy and social state. Unfortunately, Russia has a very low rate of economy socialization as a result of economic crime. The study was based on various sociological and criminological approaches. Its objective was to conduct a comparative analysis of statistical material for 2012–2019 and describe the state, trends, and consequences of economic crime in Russia and in the Republic of Bashkortostan in order to show its destructive impact on economy socialization. The research featured corruption, economic crimes, and real estate crimes. One third or even a half of all economic crimes are classified as serious or extremely serious and cause great material damage. If properly implemented, the economy socialization can create conditions for a more successful counteraction to economic crime. The results of the study can be useful in road-mapping regional socio-economic development, combating economic crime, or as comparative material for related research.
Strategies for Young College Teachers' Self-Realization
Authors: Ushakov Dmitriy
Published: October 2020

The research into social peculiarities, structure, and mechanisms of young teachers' self-realization is a relevant issue in modern sociological studies. The present paper features professional strategies that self-realization patterns. The research objective was to study strategies for professional self-realization employed by young teaching personnel of colleges. It was based on an authentic hybrid research complex, which included a combination of quantitative and qualitative analysis techniques: methods of included observation, in-depth interviews, expert interviews, and content analysis of regulatory documents. The analysis revealed three types of adaptive strategies for professional self-realization of young teachers: Strategy of Success, Strategy of Remonstrative Self-Realization, and Strategy of Forced Self-Realization. The article emphasizes the connection between social prosperity and the chosen strategy. Adaptive strategies force young teachers to comply with expectations of their social environment, which disagrees with the concept of self-realization. The humanistic non-adaptive strategy based on a free choice of patterns and modes of the teacher's work, which makes it possible to preserve physical and psychological health and save human resources.
Water reuse in hydroponic systems: a realistic future scenario for Germany? Facts and evidence gained during a transdisciplinary research project
Authors: Winker Martina , Fischer Michaela , Bliedung Alexa , Bürgow Grit , Germer Jörn , Mohr Marius , Nink Andreas , Schmitt Bea , Wieland Arne , Dockhorn Thomas
Published: October 2020

The HypoWave transdisciplinary research project investigated the innovation of water reuse in a hydroponic system, focusing on its applicability in Germany. The methods applied were the operation of a pilot plant for the appropriate treatment and subsequent reuse of water in a hydroponic system, expert interviews, feasibility studies, an impact assessment and a stakeholder dialogue. To identify the concept's advantages and disadvantages, publications specialising in the various disciplines involved and meeting protocols were analysed and the results grouped together in a SWOT (strengths, weaknesses, opportunities and threats) analysis. This revealed that the system has potential for application in Germany subject to certain requirements being met, such as suitable local wastewater treatment conditions and actors looking for new business opportunities within agricultural production. This system is not recommended for the country as a whole, but it does offer an interesting alternative for locations that meet the appropriate conditions. Nevertheless, additional efforts and knowledge are required to promote and operate a new system of this kind. These include the reliable supply of irrigation water with guaranteed plant nutrition, comprehensive quality management to manage potential risks, a good understanding of the cooperation arrangements required and a more detailed examination of energy aspects.
Understanding the perception and hierarchy of risks: an environmental medico-scientific perspective with COVID-19 in mind
Authors:   University of Strasbourg, 3 rue du loir, Oberhausbergen, Strasbourg, 67205, France, Reis J. , Zaitseva N.V. ,   Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, 82 Monastyrskaya Str., Perm, 614045, Russian Federation, Spencer P.S. ,   Oregon Health & Science University, Portland, Oregon 97201, USA
Published: October 2020

The purpose of this article is to examine risk perception among some specific stakeholders, including international intergovernmental bodies, private western-based corporates, and among European public opinion surveys. We also address concerns of the Russian Federation and the impact of China’s Belt and Road Initiative. Perception of risks is key to policy decision-making and probably more important than risk assessment. We offer a medico-scientific perspective based on factual evidence drawn mostly from official websites and publications. Our first goal is to understand if sufficient attention is given to human health, disease prevention and control relative to economic and financial considerations. Our second goal is to promote a translational and interprofessional approach to global risk prioritization by cooperation between the medicoscientific community and the financial-economic world. To this end, we examine the benefits for the practice of economic risk assessment of engaging biomedical expertise focused on global environmental health. Maintenance, expansion and sustainability of the human enterprise require health and wellness.
Priority risk factors related to drinking water from centralized water supply system that create negative trends in population health
Authors:   Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, 82 Monastyrskaya Str., Perm, 614045, Russian Federation, Kleyn S.V. , Vekovshinina S.A. ,   Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, 82 Monastyrskaya Str., Perm, 614045, Russian Federation
Published: October 2020

Our research object was long-term dynamics of parameters describing drinking water from centralized water supply systems and additional cases of health disorders among population in Russia directly caused by low-quality drinking water. Our research goal was to perform hygienic assessment of priority risk factors related to drinking water and potential health disorders that could be caused by them. Our research technique was hygienic analysis of drinking water parameters as per data taken from the federal statistical form No. 18 entitled «Data on sanitary situation in a RF region» over 2000–2019, the federal information fund of social and hygienic monitoring over 2012–2019, and calculation of associated health disorders as per MG 5.1.0095–14. Specific weight of centralized water supply sources that didn’t conform to sanitary-epidemiologic requirements decreased by 4.7 % over 2000–2019 and amounted to 14.9 %. Over the last 20 years there has been a descending trend in specific weight of water samples taken from centralized water supply sources that don’t conform to sanitary requirements as per sanitary-chemical parameters (by 2.7 %) and microbiological parameters (by 4.8%). Over 2000–2019, specific weight of water distribution networks not conforming to sanitary-epidemiologic requirements decreased by 10.7 % and amounted to 15.9 % in 2019. Over the last 20 years there has been an increase in quality of drinking water taken from centralized distribution networks. Specific weight of water samples from centralized distribution networks not conforming to sanitary requirements as per microbiological and sanitary-chemical parameters fell by 6.7 % and 7.9 % respectively. In 2012–2019 in the RF hygienic parameters of drinking water quality were the most violated as per contents of bromine, silicon, chlorine, iron, sodium, lithium, magnesium, manganese, chloroform, boron, strontium, sulfides, and hydrogen sulphide. Overall, in the RF in 2019 more than 1.66 million cases of digestive diseases, cardiovascular diseases, diseases of skin and subcutaneous tissue, and other health disorders were directly related to drinking water being contaminated with chemicals and microbiological agents; it was by 13.3 % lower than in 2012 regarding morbidity associated with drinking water quality. Priority risk factors were chlorine, chlorine organic compounds (COC), ammonia, iron, manganese, arsenic, nickel, copper, boron, magnesium, and other compounds.
Integral assessment of food products contamination with priority polyaromatic hydrocarbons
Authors:   Scientific Practical Centre of Hygiene, 8 Akademicheskaya Str., Minsk, 220012, Belarus, Dalhina N.A. , Fedorenko E.V. ,   Scientific Practical Centre of Hygiene, 8 Akademicheskaya Str., Minsk, 220012, Belarus, Sychik S.I. ,   Scientific Practical Centre of Hygiene, 8 Akademicheskaya Str., Minsk, 220012, Belarus, Belysheva L.L. ,   Scientific Practical Centre of Hygiene, 8 Akademicheskaya Str., Minsk, 220012, Belarus
Published: October 2020

Polyaromatic hydrocarbons (PAH) occur in the environment as complex mixtures and each congener has different carcinogenic and mutagenic activity. Our research goal was to accomplish an integral assessment of food products contamination with priority PAH basing on their determination with high precision procedures. We validated a procedure for determining the said substances and hygienically assessed contamination of certain food products with benzpyrene, as well as with different carcinogenic and mutagenic PAH equivalents taking into account samples with low contamination. Quantitative determination limit for benz(a)anthracene and benzpyrene was fixed at 0.01 μg/kg; benz(b)fluoranthene and chrysene, 0.1 μg/kg. Detection limit for benz(a)anthracene and benzpyrene amounted to 0.003 μg/kg in our research; for benz(b)fluoranthene and chrysene, 0.03 μg/kg. A procedure for integral assessment of contamination with the examined compounds allowed us to calculate benz(a)anthracene, benzpyrene, benz(b)fluoranthene, and chrysene contents in certain food products taking into account mixture of the examined substances, their individual contributions into aggregated contamination, and their different toxic and mutagenic activity. Median food products contamination with benzpyrene amounted to 0.0065–0.42 μg/kg; PAH taking into account carcinogenic equivalents, 0.03–0.55 μg/kg; PAH based on mutagenic equivalents, 0.04–0.81 μg/kg. Maximum concentrations of benzpyrene and PAH based on carcinogenic and mutagenic equivalents are due to a combination of subsequent technological processes that make for occurrence of the examined substances and also due to physical and chemical properties of the examined food products.
Hygienic assessment of contribution made by cooling meteorological factors into occupational risks of health disorders for workers who have to work outdoors in cold season
Authors:   North-Western State Medical University named after I.I. Mechnikov, 41 Kirochnaya Str., Saint Petersburg, 195067, Russian Federation, Polyakova E.M. ,   North-West Scientific Center for Hygiene and Public Health, 4 2 Sovetskaya Str., Saint Petersburg, 191036, Russian Federation, Mel'tser A.V. ,   North-Western State Medical University named after I.I. Mechnikov, 41 Kirochnaya Str., Saint Petersburg, 195067, Russian Federation, Chashchin V.P. ,   North-Western State Medical University named after I.I. Mechnikov, 41 Kirochnaya Str., Saint Petersburg, 195067, Russian Federation,   North-West Scientific Center for Hygiene and Public Health, 4 2 Sovetskaya Str., Saint Petersburg, 191036, Russian Federation, Erastova N.V. ,   North-Western State Medical University named after I.I. Mechnikov, 41 Kirochnaya Str., Saint Petersburg, 195067, Russian Federation
Published: October 2020

Effects produced by hazardous occupational factors on workers employed in oil extraction often occur under low temperatures when workers have to perform their tasks outdoors. Our research goal was to assess a contribution made by cooling meteorological factors into health risks for workers performing their job tasks outdoors during a cold season in order to substantiate priority prevention activities. The research was performed on workers employed by a large oil extracting company who spent different amount of time outdoors during a cold season. A posteriori group risk was assessed according to the Guide as per results obtained via periodical medical examinations in 2017–2018. Working conditions were assessed basing on a report obtained via special assessment of working conditions and industrial control results. Cooling microclimate was assessed according to G 2.2.2006-05. Basing on the results of a priori group risk assessment, work places were ranked as per health disorders probability. It was shown that in-plant noise was the leading factor causing health risks. Besides, when working experience exceeded 20 years, cooling meteorological conditions also made a substantial contribution into risks occurrence. Obtained results indicate that it is necessary to develop medical and prevention activities for workers who have to spend a lot of time outdoors in areas with cold climatic conditions.
Complex approach to assessing risks and preventing various somatic diseases in workers (employed at furniture production)
Authors:   Federal Service for Surveillance over Consumer Rights Protection and Human Wellbeing, Saratov Region office, 7 Vol'skaya Str., Saratov, 410028, Russian Federation, Merkulova N.A. , Eliseev Yu.Yu. ,   Saratov State Medical University named after V.I. Razumovsky, 112 Bol'shaya Kazach'ya Str., Saratov, 410012, Russian Federation, Kozhanova O.I. ,   Federal Service for Surveillance over Consumer Rights Protection and Human Wellbeing, Saratov Region office, 7 Vol'skaya Str., Saratov, 410028, Russian Federation
Published: October 2020

At present a number of contemporary furniture production enterprises is growing fast in Saratov region. And simultaneously there is a growth in number of working places that require adequate sanitary-hygienic assessment of their safety. Our research goal was to perform complex assessment of somatic diseases risks for workers employed at furniture production. We examined 323 workers employed at «Maria» furniture factory. To perform assessment, we applied sanitary-hygienic, laboratory-instrumental, and statistic research techniques. It was established that 23 % workers had to work under hazardous working conditions (hazard category 3.2); 37 % workers had to work under conditions belonging to hazard category 3.1; 39 % examined workers had optimal and acceptable conditions at their workplaces (category 1–2). Having examined workers’ age groups, we revealed that workers aged 21–30 prevailed among the examined ones and average working experience amounted to 5.4 years for people of this age. The second in number were workers aged 31–40 with their working experience being equal to 8.5 years. At the next stage in our research we examined morbidity among workers employed at furniture production. Thus, it was established that diseases of the nervous system were the most frequent among the examined workers (33.3 %); they were followed by respiratory diseases (20.4 %), cardiovascular diseases (12.1 %), and digestive organs diseases (10.2 %). The third stage involved examining hereditary burdens on morbidity among workers employed at furniture production. Thus, 108 workers were established to have various diseases and 79.6 % of them had hereditary predisposition. At the final stage in our research we examined retrospect data on changes in workers’ life quality over 2015. The obtained data were compared with occurrence or absence of new diseases cases over the next three years.