bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of spontaneous brain activity in distinguishing parkinsonian variant of multiple system atrophy from Parkinson’s disease at an early stage
Authors: Wang Shichan , Xiao Yi , Hou Yanbing , Li Chunyu , Zhang Lingyu , Ou Ruwei , Wei Qianqian , Lin Junyu , Yang Tianmi , Che Ningning , Jiang Qirui , Zheng Xiaoting , Liu Jiyong , Shang Huifang
Published: August 2024

Background

The overlapping clinical manifestations in parkinsonian variant of multiple system atrophy (MSA-P) and Parkinson’s Disease (PD) can complicate clinical diagnostic accuracy, particularly in the early stage. The study aims to uncover the patterns of brain function in the initial phase of the two conditions.

Methods

We recruited 24 MSA-P patients, 34 PD patients and 27 healthy controls (HC). Voxel-wise fractional amplitude of low-frequency fluctuation (fALFF) was compared to characterize regional brain function, followed by seed-based functional connectivity (FC) analysis. Receiver operating characteristic (ROC) analyses were used to examine the diagnostic accuracy of fALFF.

Results

Compared to HC, decreased fALFF was observed in the bilateral basal ganglia (BG) of MSA-P patients, while decreased fALFF was identified in the left BG of PD patients. Additionally, elevated fALFF was found in the superior cerebellum for MSA-P patients and the temporo-occipital cortex for PD patients. Furthermore, PD patients exhibited increased FC in the cortico-striatal loop compared to MSA-P patients. The fALFF of the left caudate distinguished MSA-P from HC with an area under the curve (AUC) of 0.838 (p < 0.001) and from PD with an AUC of 0.772 (p < 0.001). The fALFF of the left putamen distinguished PD from HC with an AUC of 0.736 (p = 0.002).

Conclusion

Our findings indicated common and distinct abnormalities in spontaneous brain activity within BG, cerebellum, and cortices in early-stage MSA-P and PD patients. PD patients employed more compensatory mechanisms than MSA-P patients. Furthermore, fALFF may aid in early differentiation between MSA-P and PD.
c-Jun N-terminal kinase signaling in aging
Authors: Li Yihao , You Li , Nepovimova Eugenie , Adam Vojtech , Heger Zbynek , Jomova Klaudia , Valko Marian , Wu Qinghua , Kuca Kamil
Published: August 2024

Aging encompasses a wide array of detrimental effects that compromise physiological functions, elevate the risk of chronic diseases, and impair cognitive abilities. However, the precise underlying mechanisms, particularly the involvement of specific molecular regulatory proteins in the aging process, remain insufficiently understood. Emerging evidence indicates that c-Jun N-terminal kinase (JNK) serves as a potential regulator within the intricate molecular clock governing aging-related processes. JNK demonstrates the ability to diminish telomerase reverse transcriptase activity, elevate β-galactosidase activity, and induce telomere shortening, thereby contributing to immune system aging. Moreover, the circadian rhythm protein is implicated in JNK-mediated aging. Through this comprehensive review, we meticulously elucidate the intricate regulatory mechanisms orchestrated by JNK signaling in aging processes, offering unprecedented molecular insights with significant implications and highlighting potential therapeutic targets. We also explore the translational impact of targeting JNK signaling for interventions aimed at extending healthspan and promoting longevity.
Magnitude and predictors of elasticity of demand for morphine are similar in male and female rats
Authors: Harris Andrew C. , Muelken Peter , Liu Shirelle X. , Smethells John R. , LeSage Mark G. , Gewirtz Jonathan C.
Published: August 2024

Introduction

Sex differences in vulnerability to opioid use disorder (OUD) have been reported in some clinical and preclinical studies, but findings are mixed and further research is needed in this area. The goal of this study was to compare elasticity of demand (reinforcement efficacy) in an i.v. morphine self-administration (SA) model in male and female rats using a translationally relevant behavioral economics approach. Rate of acquisition and predictors of individual differences in demand (e.g., cumulative morphine infusions during acquisition) were also evaluated in both sexes.

Materials, methods, and results

Acquisition of morphine SA (0.4 mg/kg/infusion) under a fixed ratio (FR) 1 schedule of reinforcement was slower and infusions earned were lower in females than in males (n = 30–31/sex), but infusions earned did not differ between sexes during the FR 2 and FR 3 phases of acquisition. Increases in the FR response requirement across sessions during demand testing (FR 1–FR 96) resulted in a progressive reduction in morphine infusions in both sexes. Morphine consumption was well-described by an exponential demand function in both sexes and was associated with considerable individual vulnerability. There were no sex differences in elasticity of demand (rate of decline in morphine consumption with increasing price) or intensity of demand (consumption at zero price). A higher number of infusions earned during the FR 2 and FR 3 phases of acquisition and greater maximum response rates during demand testing were associated with lower demand elasticity (i.e., greater reinforcing efficacy) in both males and females, whereas other relationships were sex-specific (e.g., higher intensity of demand was associated with lower elasticity of demand in males but not in females).

Conclusion

Our findings indicate similar elasticity of demand and predictors of individual differences in demand for morphine in male and female rats, although sex differences were observed in initial rate of acquisition and in some correlations between morphine SA measures. These data are consistent with findings of similar OUD vulnerability in males and females in some human and animal studies.
Post-weaning social isolation alters sociability in a sex-specific manner
Authors: Myers Teneisha , Birmingham Elizabeth A. , Rhoads Brigham T. , McGrath Anna G. , Miles Nylah A. , Schuldt Carmen B. , Briand Lisa A.
Published: August 2024

Adolescence is a critical period for brain development in humans and stress exposure during this time can have lasting effects on behavior and brain development. Social isolation and loneliness are particularly salient stressors that lead to detrimental mental health outcomes particularly in females, although most of the preclinical work on social isolation has been done in male animals. Our lab has developed a model of post-weaning adolescent social isolation that leads to increased drug reward sensitivity and altered neuronal structure in limbic brain regions. The current study utilized this model to determine the impact of adolescent social isolation on a three-chamber social interaction task both during adolescence and adulthood. We found that while post-weaning isolation does not alter social interaction during adolescence (PND45), it has sex-specific effects on social interaction in young adulthood (PND60), potentiating social interaction in male mice and decreasing it in female mice. As early life stress can activate microglia leading to alterations in neuronal pruning, we next examined the impact of inhibiting microglial activation with daily minocycline administration during the first 3 weeks of social isolation on these changes in social interaction. During adolescence, minocycline dampened social interaction in male mice, while having no effect in females. In contrast, during young adulthood, minocycline did not alter the impact of adolescent social isolation in males, with socially isolated males exhibiting higher levels of social interaction compared to their group housed counterparts. In females, adolescent minocycline treatment reversed the effect of social isolation leading to increased social interaction in the social isolation group, mimicking what is seen in naïve males. Taken together, adolescent social isolation leads to sex-specific effects on social interaction in young adulthood and adolescent minocycline treatment alters the effects of social isolation in females, but not males.
Vasopressin differentially modulates the excitability of rat olfactory bulb neuron subtypes
Authors: Suyama Hajime , Bianchini Gaia , Lukas Michael
Published: August 2024

Vasopressin (VP) plays a crucial role in social memory even at the level of the olfactory bulb (OB), where OB VP cells are activated during social interactions. However, it remains unclear how VP modulates olfactory processing to enable enhanced discrimination of very similar odors, e.g., rat body odors. Thus far, it has been shown that VP reduces firing rates in mitral cells (MCs) during odor presentation in vivo and decreases the amplitudes of olfactory nerve-evoked excitatory postsynaptic potentials (ON-evoked EPSPs) in external tufted cells in vitro. We performed whole-cell patch-clamp recordings and population Ca2+ imaging on acute rat OB slices. We recorded ON-evoked EPSPs as well as spontaneous inhibitory postsynaptic currents (IPSCs) from two types of projection neurons: middle tufted cells (mTCs) and MCs. VP bath application reduced the amplitudes of ON-evoked EPSPs and the frequencies of spontaneous IPSCs in mTCs but did not change those in MCs. Therefore, we analyzed ON-evoked EPSPs in inhibitory interneurons, i.e., periglomerular cells (PGCs) and granule cells (GCs), to search for the origin of increased inhibition in mTCs. However, VP did not increase the amplitudes of evoked EPSPs in either type of interneurons. We next performed two-photon population Ca2+ imaging in the glomerular layer and the superficial GC layer of responses to stronger ON stimulation than during patch-clamp experiments that should evoke action potentials in the measured cells. We observed that VP application increased ON-evoked Ca2+ influx in juxtaglomerular cells and GC somata. Thus, our findings indicate inhibition by VP on projection neurons via strong ON input-mediated inhibitory interneuron activity. This neural modulation could improve representation of odors, hence, better discriminability of similar odors, e.g., conspecific body odors.
Novel insights into mechanisms of inhibition of colonic motility by loperamide
Authors: Parkar Nabil , Spencer Nick J. , Wiklendt Luke , Olson Trent , Young Wayne , Janssen Patrick , McNabb Warren C. , Dalziel Julie E.
Published: August 2024

Background

It is well known that opiates slow gastrointestinal (GI) transit, via suppression of enteric cholinergic neurotransmission throughout the GI tract, particularly the large intestine where constipation is commonly induced. It is not clear whether there is uniform suppression of enteric neurotransmission and colonic motility across the full length of the colon. Here, we investigated whether regional changes in colonic motility occur using the peripherally-restricted mu opioid agonist, loperamide to inhibit colonic motor complexes (CMCs) in isolated mouse colon.

Methods

High-resolution video imaging was performed to monitor colonic wall diameter on isolated whole mouse colon. Regional changes in the effects of loperamide on the pattern generator underlying cyclical CMCs and their propagation across the full length of large intestine were determined.

Results

The sensitivity of CMCs to loperamide across the length of colon varied significantly. Although there was a dose-dependent inhibition of CMCs with increasing concentrations of loperamide (10 nM - 1 μM), a major observation was that in the mid and distal colon, CMCs were abolished at low doses of loperamide (100 nM), while in the proximal colon, CMCs persisted at the same low concentration, albeit at a significantly slower frequency. Propagation velocity of CMCs was significantly reduced by 46%. The inhibitory effects of loperamide on CMCs were reversed by naloxone (1 μM). Naloxone alone did not change ongoing CMC characteristics.

Discussion

The results show pronounced differences in the inhibitory action of loperamide across the length of large intestine. The most potent effect of loperamide to retard colonic transit occurred between the proximal colon and mid/distal regions of colon. One of the possibilities as to why this occurs is because the greatest density of mu opioid receptors are located on interneurons responsible for neuro-neuronal transmission underlying CMCs propagation between the proximal and mid/distal colon. The absence of effect of naloxone alone on CMC characteristics suggest that the mu opioid receptor has little ongoing constitutive activity under our recording conditions.
Differentiation stage-specific expression of transcriptional regulators for epithelial mesenchymal transition in dentate granule progenitors
Authors: Ohyama Kyoji , Shinohara Hiroshi M. , Takayama Natsumi , Ogawa Rina , Omura Shoichiro , Hayashida Mio , Takahashi Tokiharu
Published: August 2024

During the development of the mouse dentate gyrus (DG), granule neuronal progenitors (GNPs) arise from glial fibrillary acidic protein (GFAP)-expressing neural stem cells in the dentate notch. However, the transcriptional regulators that control their stepwise differentiation remain poorly defined. Since neurogenesis involves epithelial-to-mesenchymal transition (EMT)-like processes, we investigated the spatio-temporal expression profiles of the EMT transcription factors Zeb1, Scratch2 (Scrt2) and Nkx6-2 in relation to known GNP markers. Our results show that Zeb1 and Scrt2 exhibit sequential, but partially overlapping expression across embryonic and postnatal stages of GNP differentiation. Zeb1 is highly enriched in gfap-GFP+/Sox2+ neural stem/progenitor pools and subsets of Tbr2+/Prox1+/NeuroD+ intermediate GNPs, whereas Scrt2 predominates in Tbr2+/Prox1+/NeuroD+ GNPs. Strikingly, the neuronal EMT regulator Nkx6-2 shows selective expression in postnatal Tbr2+/Prox1+ GNPs, but it is excluded from embryonic counterparts. This temporally coordinated yet distinct expression of Zeb1, Scrt2 and Nkx6-2 reveals discrete transcriptional programs orchestrating GNP differentiation and neurogenic progression at embryonic versus postnatal stages of DG neurogenesis.
On the basis of sex and sleep: the influence of the estrous cycle and sex on sleep-wake behavior
Authors: Swift Kevin M. , Gary Nicholas C. , Urbanczyk Phillip J.
Published: August 2024

The recurrent hormonal fluctuations within reproductive cycles impact sleep-wake behavior in women and in rats and mice used in preclinical models of sleep research. Strides have been made in sleep-related clinical trials to include equal numbers of women; however, the inclusion of female rodents in neuroscience and sleep research is lacking. Female animals are commonly omitted from studies over concerns of the effect of estrus cycle hormones on measured outcomes. This review highlights the estrous cycle’s broad effects on sleep-wake behavior: from changes in sleep macroarchitecture to regionally specific alterations in neural oscillations. These changes are largely driven by cycle-dependent ovarian hormonal fluctuations occurring during proestrus and estrus that modulate neural circuits regulating sleep-wake behavior. Removal of estrous cycle influence by ovariectomy ablates characteristic sleep changes. Further, sex differences in sleep are present between gonadally intact females and males. Removal of reproductive hormones via gonadectomy in both sexes mitigates some, but not all sex differences. We examine the extent to which reproductive hormones and sex chromosomes contribute to sex differences in sleep-wake behavior. Finally, this review addresses the limitations in our understanding of the estrous cycle’s impact on sleep-wake behavior, gaps in female sleep research that are well studied in males, and the implications that ignoring the estrous cycle has on studies of sleep-related processes.
Does tinnitus amplify the effects of healthy eating patterns and physical activity on the sleep disturbance or sleep insufficiency, based on the case study of NHANES survey in the United States
Authors: Chen Jia , Zou Wujun , Li Hao , Luo Yu , Lu Kaifu , Yi Xuelian , Li Hong , Shi Zhu , Meng Juan
Published: August 2024

Objective

Exploring whether the presence of tinnitus amplifies the effects of an individual’s dietary patterns and physical activity on sleep disturbance or sleep insufficiency.

Study design

This study extracted data from the five National Health and Nutrition Examination Surveys (NHANES) between 2009 and 2018, including individuals who had undergone complete questionnaires on tinnitus, dietary habits, physical activity, and sleep. Multivariate logistic regression, restricted cubic spline (RCS) and subgroup analyses were conducted to explore the associations of dietary habits, physical activity, and tinnitus with sleep disturbance and sleep insufficiency.

Results

A total of 7,440 participants were enrolled in this study, of whom 1,795 participants were evaluated as sleep disturbance (24.13%), and 2,281 were sleep insufficiency (30.66%). With adjusting confounding factors of demographic and socioeconomic variables, among overall population, participants with tinnitus showed a significantly increased risk of sleep disturbance [adjusted odds ratio (aOR) = 2.08, 95% confidence interval (CI): 1.83–2.36), and sleep insufficiency (aOR = 1.31, 95% CI: 1.15–1.49). Poor dietary habits also increased the risk of sleep disturbance (aOR = 1.08, 95% CI: 1.04–1.12), as does lack of physical activity (aOR = 1.14, 95% CI: 1.03–1.27); but neither exposure factors significantly increased the risk of sleep insufficiency. The non-linear trend analyses of RCS found that the influence of exposure factors on sleep disturbance experiencing a steady or small decline trend after rising. In addition, the results of the subgroup analysis showed that in tinnitus patients, poor dietary habits and lack of physical activity both significantly increased the risk of sleep disturbance, and poor dietary habits also increased the risk of sleep insufficiency remarkable, but lack of physical activity did not. In healthy participants, poor dietary habits were only significantly associated the sleep disturbance, while lack of physical inactivity even had a protective effect against sleep insufficiency.

Conclusion

Compared to the general population, tinnitus significantly amplified the effects of poor dietary patterns and physical inactivity on sleep disturbance and sleep insufficiency. For tinnitus patients, adjusting a healthy diet and increasing exercise could more effectively promote their sleep health.
Using multimodal ultrasound including full-time-series contrast-enhanced ultrasound cines for identifying the nature of thyroid nodules
Authors: He Hanlu , Zhu Junyan , Ye Zhengdu , Bao Haiwei , Shou Jinduo , Liu Ying , Chen Fen
Published: August 2024

Background

Based on the conventional ultrasound images of thyroid nodules, contrast-enhanced ultrasound (CEUS) videos were analyzed to investigate whether CEUS improves the classification accuracy of benign and malignant thyroid nodules using machine learning (ML) radiomics and compared with radiologists.

Materials and methods

The B-mode ultrasound (B-US), real-time elastography (RTE), color doppler flow images (CDFI) and CEUS cines of patients from two centers were retrospectively gathered. Then, the region of interest (ROI) was delineated to extract the radiomics features. Seven ML algorithms combined with four kinds of radiomics data (B-US, B-US + CDFI + RTE, CEUS, and B-US + CDFI + RTE + CEUS) were applied to establish 28 models. The diagnostic performance of ML models was compared with interpretations from expert and nonexpert readers.

Results

A total of 181 thyroid nodules from 181 patients of 64 men (mean age, 42 years +/- 12) and 117 women (mean age, 46 years +/- 12) were included. Adaptive boosting (AdaBoost) achieved the highest area under the receiver operating characteristic curve (AUC) of 0.89 in the test set among 28 models when combined with B-US + CDFI + RTE + CEUS data and an AUC of 0.72 and 0.66 when combined with B-US and B-US + CDFI + RTE data. The AUC achieved by senior and junior radiologists was 0.78 versus (vs.) 0.69 (p > 0.05), 0.79 vs. 0.64 (p < 0.05), and 0.88 vs. 0.69 (p < 0.05) combined with B-US, B-US+CDFI+RTE and B-US+CDFI+RTE+CEUS, respectively.

Conclusion

With the addition of CEUS, the diagnostic performance was enhanced for all seven classifiers and senior radiologists based on conventional ultrasound images, while no enhancement was observed for junior radiologists. The diagnostic performance of ML models was similar to senior radiologists, but superior to those junior radiologists.
2.5D peritumoural radiomics predicts postoperative recurrence in stage I lung adenocarcinoma
Authors: Lan Haimei , Wei Chaosheng , Xu Fengming , Yang Eqing , Lu Dayu , Feng Qing , Li Tao
Published: August 2024

Objective

Radiomics can non-invasively predict the prognosis of a tumour by applying advanced imaging feature algorithms.The aim of this study was to predict the chance of postoperative recurrence by modelling tumour radiomics and peritumour radiomics and clinical features in patients with stage I lung adenocarcinoma (LUAD).

Materials and methods

Retrospective analysis of 190 patients with postoperative pathologically confirmed stage I LUAD from centre 1, who were divided into training cohort and internal validation cohort, with centre 2 added as external validation cohort. To develop a combined radiation-clinical omics model nomogram incorporating clinical features based on images from low-dose lung cancer screening CT plain for predicting postoperative recurrence and to evaluate the performance of the nomogram in the training cohort, internal validation cohort and external validation cohort.

Results

A total of 190 patients were included in the model in centre 1 and randomised into a training cohort of 133 and an internal validation cohort of 57 in a ratio of 7:3, and 39 were included in centre 2 as an external validation cohort. In the training cohort (AUC=0.865, 95% CI 0.824-0.906), internal validation cohort (AUC=0.902, 95% CI 0.851-0.953) and external validation cohort (AUC=0.830,95% CI 0.751-0.908), the combined radiation-clinical omics model had a good predictive ability. The combined model performed significantly better than the conventional single-modality models (clinical model, radiomic model), and the calibration curve and decision curve analysis (DCA) showed high accuracy and clinical utility of the nomogram.

Conclusion

The combined preoperative radiation-clinical omics model provides good predictive value for postoperative recurrence in stage ILUAD and combines the model’s superiority in both internal and external validation cohorts, demonstrating its potential to aid in postoperative treatment strategies.
Neighborhood attention transformer multiple instance learning for whole slide image classification
Authors: Aftab Rukhma , Yan Qiang , Zhao Juanjuan , Yong Gao , Huajie Yue , Urrehman Zia , Mohammad Khalid Faizi
Published: August 2024

Introduction

Pathologists rely on whole slide images (WSIs) to diagnose cancer by identifying tumor cells and subtypes. Deep learning models, particularly weakly supervised ones, classify WSIs using image tiles but may overlook false positives and negatives due to the heterogeneous nature of tumors. Both cancerous and healthy cells can proliferate in patterns that extend beyond individual tiles, leading to errors at the tile level that result in inaccurate tumor-level classifications.

Methods

To address this limitation, we introduce NATMIL (Neighborhood Attention Transformer Multiple Instance Learning), which utilizes the Neighborhood Attention Transformer to incorporate contextual dependencies among WSI tiles. NATMIL enhances multiple instance learning by integrating a broader tissue context into the model. Our approach enhances the accuracy of tumor classification by considering the broader tissue context, thus reducing errors associated with isolated tile analysis.

Results

We conducted a quantitative analysis to evaluate NATMIL’s performance against other weakly supervised algorithms. When applied to subtyping non-small cell lung cancer (NSCLC) and lymph node (LN) tumors, NATMIL demonstrated superior accuracy. Specifically, NATMIL achieved accuracy values of 89.6% on the Camelyon dataset and 88.1% on the TCGA-LUSC dataset, outperforming existing methods. These results underscore NATMIL’s potential as a robust tool for improving the precision of cancer diagnosis using WSIs.

Discussion

Our findings demonstrate that NATMIL significantly improves tumor classification accuracy by reducing errors associated with isolated tile analysis. The integration of contextual dependencies enhances the precision of cancer diagnosis using WSIs, highlighting NATMILs´ potential as a robust tool in pathology.
The role of lymphocyte-C-reactive protein ratio in the prognosis of gastrointestinal cancer: a systematic review and meta-analysis
Authors: Liu XiaoMeng , Zhang JingChen , An HaoYu , Wang WanYao , Zheng YuKun , Wei FengJiang
Published: August 2024

Objective

The lymphocyte-to-C-reactive protein (LCR) ratio, an immune-inflammatory marker, shows prognostic potential in various cancers. However, its utility in gastrointestinal malignancies remains uncertain due to inconsistent findings. This systematic review and meta-analysis synthesizes recent evidence to elucidate the association between LCR and prognosis in gastrointestinal cancer patients, aiming to clarify LCR’s potential role as a prognostic biomarker.

Methods

We searched PubMed, Embase, Cochrane, and Web of Science databases up to May 2024 to evaluate the association between LCR and prognosis in gastrointestinal cancer patients. The main outcomes included overall survival (OS), recurrence-free survival (RFS), and disease-free survival (DFS). We also analyzed secondary parameters such as geographical region, study duration, sample size, LCR threshold, and patient characteristics (age, gender, tumor location, and TNM stage).

Results

This meta-analysis of 21 cohort studies (n=9,131) finds a significant association between reduced LCR levels and poor prognosis in gastrointestinal cancer. Lower LCR levels were associated with worse overall survival (HR=2.01, 95% CI=1.75-2.31, P<0.001), recurrence-free survival (HR=1.90, 95% CI=1.32-2.76, P<0.001), and disease-free survival (HR=1.76, 95% CI=1.45-2.13, P<0.001). Subgroup analyses by cancer type, timing, and LCR threshold consistently confirmed this relationship (P<0.05).

Conclusion

LCR may serve as a prognostic marker in gastrointestinal cancer patients, with lower LCR levels associated with poorer prognosis. However, more high-quality studies are needed to validate these findings, considering the limitations of the current evidence.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier CRD42023486858.
Long non-coding RNAs in ferroptosis, pyroptosis and necroptosis: from functions to clinical implications in cancer therapy
Authors: Huang Ke , Yu Li , Lu Dingci , Zhu Ziyi , Shu Min , Ma Zhaowu
Published: August 2024

As global population ageing accelerates, cancer emerges as a predominant cause of mortality. Long non-coding RNAs (lncRNAs) play crucial roles in cancer cell growth and death, given their involvement in regulating downstream gene expression levels and numerous cellular processes. Cell death, especially non-apoptotic regulated cell death (RCD), such as ferroptosis, pyroptosis and necroptosis, significantly impacts cancer proliferation, invasion and metastasis. Understanding the interplay between lncRNAs and the diverse forms of cell death in cancer is imperative. Modulating lncRNA expression can regulate cancer onset and progression, offering promising therapeutic avenues. This review discusses the mechanisms by which lncRNAs modulate non-apoptotic RCDs in cancer, highlighting their potential as biomarkers for various cancer types. Elucidating the role of lncRNAs in cell death pathways provides valuable insights for personalised cancer interventions.
The prognostic value of preoperative peripheral blood inflammatory biomarkers in extrahepatic cholangiocarcinoma: a systematic review and meta-analysis
Authors: Zeng Di , Wang Yaoqun , Wen Ningyuan , Lu Jiong , Li Bei , Cheng Nansheng
Published: August 2024

Background

Recent evidence indicates that inflammation plays a major role in the pathogenesis and progression of CCA. This meta-analysis seeks to evaluate the prognostic implications of preoperative inflammatory markers, specifically NLR, PLR, and LMR, in patients with eCCA. By focusing on these preoperative biomarkers, this study aims to provide valuable insights into their prognostic value and potential utility in clinical practice.

Methods

For this analysis, comprehensive searches were conducted in PubMed, Embase, and Web of Science databases from inception to May 2024. The primary outcomes of interest focused on the association between the levels of NLR, PLR, and LMR and the prognosis of eCCA patients. Statistical analyses were conducted using STATA 17.0 software.

Results

The meta-analysis, involving 20 retrospective studies with 5553 participants, revealed significant correlations between preoperative biomarkers and the prognosis of eCCA patients. Elevated NLR, PLR, and decreased LMR levels were extensively studied regarding overall survival (OS) in eCCA patients. Elevated NLR was an independent predictor of poor OS (HR 1.86, p < 0.001), similar to elevated PLR (HR 1.76, p < 0.001), while decreased LMR predicted poor OS (HR 2.16, p < 0.001). Subgroup analyses based on eCCA subtypes and curative surgery status showed consistent results.

Conclusions

In conclusion, our study emphasizes the clinical significance of assessing NLR, PLR, and LMR preoperatively to predict patient prognosis. Elevated NLR and PLR values, along with decreased LMR values, were linked to poorer overall survival (OS). Large-scale prospective cohort studies are required to confirm their independent prognostic value in eCCA.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier CRD42024551031.
Skipping breakfast is associated to inadequate nutrient intakes among Tunisian children: a cross-sectional study
Authors: El Ati Jalila , Doggui Radhouene , Dogui Darine , El Ati-Hellal Myriam
Published: August 2024

Introduction

Breakfast is widely acknowledged as a crucial meal of the day, particularly for children, owing to its role in supplying essential nutrients and energy necessary for optimal growth and cognitive function. This study aims to examine differences in nutrient intake between breakfast skippers and non-skippers among children.

Methods

A representative sample of 1,200 Tunisian preschool and schoolchildren, aged 3–9 years, was randomly selected from kindergartens and primary schools in the Greater Tunis region which includes four governorates (Tunis, Ariana, Manouba and Ben Arous) using a two-stage cross-sectional design. Stratification was carried out depending on each of the selected governorates and urban/rural areas. Dietary intake was evaluated using the 24-hour recall method and a diet history covering the week preceding the survey. Nutritional food composition was derived from a specific Tunisian food composition database. Nutrient intake below age- and sex-specific reference values was considered inadequate.

Results and Discussion

The findings indicate that the daily intake of energy and nutrients was lower among breakfast skippers compared to regular breakfast eaters. After adjusting for energy, gender, age, BMI and household economic proxy, the daily consumption of total sugar, riboflavin, vitamin B-5, phosphorus and calcium was higher among breakfast consumers while saturated fatty acid intake was higher among skippers. A comparative analysis of daily mean food category intake between the two groups revealed a higher consumption of milk and dairy products among breakfast consumers. Regular breakfast consumption is an important part of maintaining a balanced diet and healthy weight.
What do we need to address when we treat neglected Monteggia fracture in children
Authors: Yi Yangfei , Liu Can , Xu Zheng , Xie Yuyin , Cao Shu , Wen Jie , Jian Xiaohong , Li Yufei
Published: August 2024

Monteggia fracture is a relatively uncommon injury in pediatric patients, accounting for less than 2% of forearm fractures, characterized by a combination of ulna fracture and radial head dislocation. Neglected Monteggia fractures define as those that have not received treatment within 3 weeks. In children, ulna fractures are easily diagnosed while radial head dislocation may be overlooked, necessitating open reduction after neglecting the Monteggia fracture and potentially causing additional trauma to the child. This study aims to review the pathological characteristics of neglected Monteggia fractures based on the length ratio of the ulna and radius, relative positions between the proximal ends of the ulna and radius, the integrality of annular ligament and the pathological change of proximal radioulnar joint. The findings will provide valuable insights and guidance for managing neglected Monteggia fractures.
Democracy with adjectives? Economic development and democracy in the Middle East
Authors: Yasun Salih
Published: August 2024

Shadi Hamid, in his book “The Problem of Democracy, advocates for the promotion of minimalist democracy for the Middle East. Can minimalist democracy respond to the aspirations of the Arab public? Using data from the Arab Barometer (2018) survey, I emphasize that many citizens of Arab countries define democracy by its expected economic outcomes. Whether minimalist democracy can spur economic development is a puzzle. Literature on democratization and economic growth is inconclusive. In the Middle East and North Africa region, ballot box alone may not overcome significant barriers, such as overriding interest groups and corruption, to spur development. Democratic openings in the region often failed to promote expected economic growth. Therefore, while minimalist democracy would be an improvement for the current standards of governance in the region, it may fail to respond to the aspirations of the masses, making authoritarian reversion a likely outcome.
Women's equality in the era of permacrisis
Authors: Koutselini Mary
Published: August 2024

Permacrisis implies a permanent state of crisis, contrasting with polycrisis and intracrisis, which refer to crises in several fields that can be investigated and resolved. Women's equality has been a longstanding issue, analyzed theoretically and through research exploring persistent and pervasive inequality in all areas of life. This study discusses the concept of permacrisis from a gender perspective and presents research highlighting the effects of interconnected crises on women's rights and societal presence. Ongoing crises—economic, environmental, political, and educational—have been exacerbated by temporary wars, climate change, pandemics, and disputes over international institutions for the protection of human rights and the vulnerable populations. These issues, combined with ineffective political leadership, have made women's equality an increasingly distant goal. This situation calls for new analytical frameworks that go beyond the pessimism of permacrisis and lead to what can be termed a meta-crisis, a transcendence of current obstacles. Thus, the main aims of this study are two-fold: First, to discuss permacrisis from a gender perspective, and second, to propose research evidence showing that gender inequality as an inherent aspect of permacrisis requires new theoretical insights for effective analysis.
Impact of religion and spirituality on the incidence of depression and mental health among young adults in the Czech Republic
Authors: Brečka Tibor A. , Ptáček Radek , Sebalo Ivan , Anders Martin , Sebalo Vňuková Martina
Published: August 2024

Introduction

The issue of the impact of religion and spirituality on mental health is a phenomenon which has recently become increasingly more accentuated. Despite the attention given to the topic, many questions still remain as to whether and how religion and spirituality affect a person’s mental wellbeing. In the text below, we have focused on examining the relationship between religion and spirituality and mental health among young adults in the Czech Republic. Research also explored the idea that forgiveness can be viewed as a component of religion or spirituality.

Materials and methods

The research project was executed in close cooperation with STEM/MARK, a renowned data collection agency. The methodological framework was constructed with a dual focus: leveraging standardized questionnaires to ensure data reliability and comparability while also incorporating tailored questions that delve into the participants’ socioeconomic status (SES) and background details. The study unfolded across four online sessions, a format chosen for its convenience and effectiveness in facilitating participant engagement while accommodating our respondents’ diverse schedules. The total sample approached comprised of 270 young adults that expressed certain form of religiosity.

Results

Forgiveness and the depth of one’s personal religious or spiritual history emerged as the most influential factors. Forgiveness was significantly associated with an increase in self-blame (positively), and decrease in refocusing, planning (both negatively), and putting things into perspective (negatively) (Beta = 0.25, Beta = −0.06, and Beta = −0.16, respectively). In contrast, a deeper personal religious history was positively associated with self-blame, rumination, and refocusing (Beta = 0.22, Beta = 0.13, and Beta = 0.15, respectively).

Conclusion

The finding that forgiveness may be a risk factor associated with regularly elevated depressive symptoms, stress, and maladaptive coping strategies such as self-blame and ruminating over problems, while negatively affecting physical, psychological, and environmental quality of life, clearly points to the need to examine the inner aspects of individual religions and spiritualities. These findings suggest that religious and spiritual beliefs may play a key role in how people experience and manage the emotional burdens and difficulties of life.
Seeing others suffer and enjoying it? The Model of Individual and Social Appraisals of Misfortunes of others in media reception
Authors: Suter Lilian , Döveling Katrin
Published: August 2024

Suffering and misfortunes of other people are often portrayed in the media. Recipients react to these portrayals with different emotions. This article elucidates and clarifies schadenfreude (pleasure at the misfortune of others) and sympathy (feeling concern or sorrow over another person’s distress) in media experiences. A thorough literature review provides in-depth insights into the formation of affective dispositions and schadenfreude from various psychological perspectives. This conceptual analysis leads to the “Model of Individual and Social Appraisals of Misfortunes of Others” (MISAM) which first reveals the determining intrapersonal factors within the emotional experience of schadenfreude and sympathy. Second, it discloses the social component vital for understanding the construction and regulation of these emotions. The model combines individual and social appraisal processes and identifies the factors involved in the elicitation and regulation of schadenfreude and sympathy in the media reception of misfortunes. With the aim of integrating different perspectives, we incorporated Affective Disposition Theory and recent work from social psychology and used an appraisal framework. The MISAM opens the path for further investigation of schadenfreude and sympathy in media reception, beyond entertainment experiences.
Problematic internet use and shyness among Chinese adolescents: a moderated mediation model
Authors: Sun Hong , Yu Yang
Published: August 2024

The potential adverse effects of problematic internet use (PIU) on individuals’ offline personalities have been a significant concern in internet psychology. This study aimed to investigate the negative effects of PIU on shyness and the mediating role of self-regulation, as well as the moderating role of social comparison orientation. A total of 1,322 adolescents from China participated in the survey. The results indicated that: (1) PIU positively predicted shyness; (2) self-regulation partially mediated the relationship between PIU and shyness; (3) social comparison orientation moderated the mediation effect, specifically buffering the negative prediction of PIU on self-regulation, but exacerbating the negative predictive effect of self-regulation on shyness. The findings explored and verified the psychosocial effects of the internet, contributing to a deeper understanding of how PIU affects individual personality traits and offering insights into its role in broader social behavior patterns.
Effects of aripiprazole on prolactin levels and differences in effectiveness in patients with schizophrenia: a post-hoc analysis of the real-world data of a multicenter study
Authors: Li Qian , Su Yun-Ai , Liao Xuemei , Fang Maosheng , Gao Jianliang , Xu Jia , Duan Mingjun , Yu Haiying , Yang Yang , Chen Zhiyu , Liu Jintong , Yan Shaoxiao , Yao Peifen , Li Shuying , Wang Changhong , Wu Bin , Zhang Congpei , Si Tianmei
Published: August 2024

Objectives

To investigate the effect of aripiprazole on prolactin levels in patients with schizophrenia and analyze whether varying baseline prolactin levels affect the effectiveness and safety of aripiprazole, in a real-life diagnostic and therapeutic setting in a post-hoc analysis.

Methods

In this post-hoc analysis, patients with schizophrenia in the acute phase were divided into an elevated-prolactin group and a normal-prolactin group. After 8 weeks of aripiprazole treatment, changes in the proportion of patients with an abnormal prolactin level were analyzed in both groups, and the efficacy and safety of aripiprazole were compared between the two groups.

Results

The elevated-prolactin group had more women, a longer duration of disease, and lower Positive and Negative Syndrome Scale (PANSS) total and subscale scores at baseline compared with the normal-prolactin group (all P < 0.05), and there was no significant difference in the proportion of patients with prior use of antipsychotic medication between the two groups. Regardless of previous antipsychotic use, patients in both groups developed hyperprolactinemia (23/168 [13.7%] in those who had taken antipsychotics vs. 43/375 [11.4%] in those who had not). After 8 weeks of aripiprazole treatment, the proportion of patients with abnormal prolactin in the elevated-prolactin group significantly decreased with prolonged treatment (P < 0.001), and aripiprazole had no significant effect on the normal-prolactin group (P = 0.250). Normal-prolactin group showed better efficacy than the elevated-prolactin group, and the differences in efficacy between the two groups was observed from week 4 to the endpoint (all p<0.05). In total, 87.2% (68/78) patients experienced mild to moderate adverse events in the elevated-prolactin group, which was significantly more frequent compared with the normal-prolactin group 71.0% (365/514).

Conclusions

In this real-world study, for patients with acute schizophrenia, aripiprazole was effective in lowering the proportion of patients with abnormal prolactin levels, while it had no significant effect on patients with normal baseline prolactin. After adjusting for factors such as sex, age, prior antipsychotic drugs use history and disease severity, effectiveness and safety of aripiprazole in patients with normal baseline prolactin was significantly better than that in patients with elevated baseline prolactin.
Inverted U-shaped relationship between serum 25-hydroxyvitamin D concentrations and Toxoplasma gondii infection: a cross-sectional study
Authors: Huang Lihua , Luo Xiaoyan , He Liuliu , You Xiaoyan , Chen Xiaobo
Published: August 2024

Background

Toxoplasma gondii (T. gondii) is a widespread zoonotic parasite transmitted through contaminated food or water. It poses a significant public health threat, especially to pregnant women and immunocompromised individuals. 25-Hydroxyvitamin D [25(OH)D] plays a critical role in regulating both innate and adaptive immune responses, particularly in its anti-infective capacity. However, the relationship between serum 25(OH)D concentrations and T. gondii infection remains uncertain.

Methods

We analyzed the data from the National Health and Nutrition Examination Survey (NHANES) spanning 2009–2014 to explore the association between serum 25(OH)D concentrations and T. gondii infection. Extensive demographic, comorbidity, and dietary data were collected. The status of T. gondii infection was determined using serum anti-IgG antibodies. Serum 25(OH)D levels were measured using ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS). In addition, weighted logistic regression and restricted cubic spline analyses were performed.

Results

Our analysis included 10,157 participants (mean [SE] age, 45.38 [0.39] years; 49.73% female) who met the inclusion criteria. Serum 25(OH)D levels were categorized into quintiles, with the second quintile serving as the reference group. The final model, adjusted for age, sex, race, education level, poverty income ratio, body mass index, smoking status, hypertension, diabetes, chronic kidney disease, depression, physical activity, alcohol intake, seasonal testing, and dietary vitamin D, revealed the following adjusted odds ratios (ORs) for the quintiles: 0.75 (95% confidence interval [CI]: 0.60–0.93) for the first, 0.87 (95% CI: 0.69–1.10) for the third, 0.75 (95% CI: 0.58–0.95) for the fourth, and 0.66 (95% CI: 0.49–0.91) for the fifth. Additionally, a restricted cubic spline analysis revealed an inverted U-shaped relationship between serum 25(OH)D and T. gondii infection, with an inflection point at approximately 51.29 nmol/L. Odds ratios to the left and right of the inflection point were 1.17 (95% CI: 1.03–1.32) and 0.94 (95% CI, 0.90–0.98) per 10 nmol/L, respectively.

Conclusion

Our study uncovers an inverted U-shaped relationship between serum 25(OH)D concentrations and T. gondii infection, with an inflection point around 51.29 nmol/L.