bluuebunny/crossref_metadata_2025 huggingface dataset:
FALSO-POSITIVOS PARA HIV/AIDS EM GRÁVIDAS: ANÁLISE DE ACÓRDÃOS DO RIO DE JANEIRO
Authors: Jorge Juliana de Melo , Brito Talita Gabriele Pereira , Cerqueira Marília Borborema Rodrigues , Ravnjak Leandro Luciano Silva , Gonçalves Maria Elizete
Published: May 2024

A pesquisa visa apresentar o direito à saúde das mulheres grávidas e a ocorrência de processos civis em casos de resultados falso-positivos para diagnóstico de HIV/AIDS. O aparecimento do falso-positivo para HIV/AIDS, nos exames de pré-natal, ocorre devido ao surgimento do aloanticorpo, no processo das alterações hormonais. Para o desenvolvimento deste trabalho, foi realizado um estudo evolutivo sobre a responsabilidade civil subjetiva e objetiva, a fim de demonstrar que poderá haver situações em que o médico e o biomédico, sendo eles responsáveis pelos atos de ofício da sua profissão, podem passar por um exame com resultado falso-positivo. É vista a possibilidade de ocorrência de responsabilidade subjetiva e objetiva, para os médicos e para os biomédicos, perante o Código Civil (2002), Código de Defesa do Consumidor (1990), Código de Ética Médica e Código de Ética do Profissional Biomédico (2020). Foi realizada a pesquisa por meio de análise dos acórdãos jurisprudenciais do Tribunal de Justiça do Estado do Rio de Janeiro (TJRJ), para que o tema ora estudado se verifique no caso em concreto.
Immunomagnetic Assay Using Photon-Upconversion Nanoparticles for the Detection of Cancer Biomarkers
Authors: Sklenárová Dorota , Makhneva Ekaterina , Farka Zdeněk
Published: May 2024

Prostatic carcinoma is the most frequently diagnosed type of cancer within the male population worldwide. The most important prostate cancer biomarker is the prostate-specific antigen (PSA). Changes in PSA concentration may occur in very narrow ranges, making ultrasensitive analytical methods necessary. Enzyme-linked immunosorbent assay (ELISA) is considered the gold standard of analytical methods. It utilizes microtiter plates as solid phase and enzyme-based labels. However, ELISAs are often not sensitive enough for early-stage detection of subclinical levels of PSA. To enhance the sensitivity, it is possible to introduce suitable alternatives for both the solid phase and the label.
Our work focuses on the use of magnetic microparticles (MBs) and photon-upconversion nanoparticles (MBs) as alternatives for solid phase and the label, respectively. The superparamagnetic properties and higher specific surface of MBs allows for analyte preconcentration and simple magnetic separation of the immunocomplex. UCNPs, on the other hand, are nanocrystals exhibiting anti-Stokes luminescence (converting light in the NIR region to visible light). After surface modification, it is possible to utilize UCNPs as labels in the upconversion-linked immunosorbent assay (ULISA), significantly enhancing the sensitivity. We have developed and optimized ELISA and ULISA assays for the detection of PSA and compared their parameters with the equivalent assays utilizing MBs as solid phase. Moreover, we have developed a novel ULISA method with an additional magnetic preconcentration step to further enhance sensitivity, providing a limit of detection of 0.46 pg mL–1.
Case Study Insights: Passive Range of Motion and Extended Stretching in Improving Functional Mobility for Individuals with Spastic Cerebral Palsy
Authors: Naveen G , Aravindan T , Anishka . , Nikhat .
Published: May 2024

Cerebral Palsy (CP) is a non-progressive neuromuscular disorder of cerebral origin. It includes a number of clinical disorders, mostly arising in childhood. The essential features vary the degree of upper motor neuron type of limb paralysis (spasticity 70-80%), together with difficulty in coordination (ataxia) and purposeless movements (athetosis). A single case study 3 years old male of spastic CP & with a score of 2 on a modified Ashworth scale, Chief complaints of difficulty in neck control & delayed milestones. Passive ROM and prolonged stretching for the lower limb have been given to improve ROM, spasticity and mobility. decrease in spasticity and improvement in range of motion. There was a significant decrease in spasticity and improvement in the range of motion by using the digital goniometer and modified Ashworth scale in children with spastic cerebral palsy. Keywords: Cerebral palsy, Stretching, Spasticity
THE GOOD, BAD AND UGLY SIDES OF VALUES: IMPLICATIONS FOR NATIONAL DEVELOPMENT IN NIGERIA
Authors: UWAIFO Roseline Osayi , OMIYEFA Muraina Olugbenga
Published: May 2024

This paper examined the good, bad and ugly aspects of values and the implications on national development. The crucial nature of values cannot be overstated. Values are either good or bad but the bad values have very terrible consequences hence, graded as ugly values. The paper therefore examined what value is all about, some of the good values in Nigeria, and the attributes that help these values to strive. Furthermore, the bad and ugly values were also examined along with their attributes and the effect on the citizens. It was established that good values when entrenched will lead to national development and global acceptability while bad values will lead to bad leadership, national catastrophe, retrogression, poverty, fear, and terrorism. The paper therefore suggested punishment for those found wanting, corrupt officials should face justice, and that Social Studies should be made compulsory from primary schools to tertiary institutions.
ADJUDICATION OF MUSIC PERFORMANCES IN THREE SELECTED MUNICIPALITY/ DISTRICT IN GHANA
Authors: Mensah Augusta Arko
Published: May 2024

This paper presents an inquiry into the adjudication of music performances during cultural festivals within two municipalities and one district in the Central Region of Ghana. It examines the competence levels of adjudicators engaged in the process and elucidates the employed adjudication process, aiming to foster understanding and appreciation among all competition participants. Guided by Dworkin’s theory of interpretation, which delineates pre-interpretive, interpretive, and post-interpretive stages in adjudication, the research adopts a qualitative paradigm, utilizing interviews and document analysis as primary data collection instruments. Convenience and purposive sampling were employed to select both the geographical area and study participants respectively. The findings indicate that the selection of adjudicators is the prerogative of cultural coordinators, resulting in a less competitive process that may compromise competence in adjudication. Furthermore, although the cultural festival employs a scoring system across various disciplines to ensure precision and equity through expert adjudication, adjudicators struggle to provide comprehensive feedback that could aid participating groups in improving future performances. The paper suggests the establishment of strategies to introduce competitiveness in the engagement of adjudicators, coupled with plans to ensure their training as part of the preinterpretive stage before proceeding to the interpretive and post-interpretive stages. This approach aims to enhance the overall quality and effectiveness of the adjudication process.
INTEGRATING THE TECHNIQUES OF DIAGRAMMING AND GROUP DISCUSSION IN THE INSTRUCTION OF MARXIST-LENINIST PHILOSOPHY
Authors: Thi Giang Nguyen , Huong Bui Lan
Published: May 2024

Marxist-Leninist philosophy constitutes one of the triadic pillars within the broader framework of Marxism-Leninism, evolving concomitantly with advancements in scientific thought and the practical dynamics of the revolutionary movement of the working class. It holds the status of a mandatory subject within the curricula of higher education institutions overseen by the Ministry of Education and Training in Vietnam. This discipline imparts a scientific worldview and applies a materialist-dialectical approach to enhance cognition and pedagogy, thereby facilitating practical improvement. Hence, the instructional approach must be adaptable to ensure that lectures are comprehensible and aligned with the stated objectives. Employing a blend of teaching methodologies assists educators in effectively conveying information, facilitating a logical and cohesive understanding of the subject matter for learners. Drawing insights from research on diagramming and group discussion methods, this article formulates requisites and procedures for amalgamating diagramming with group discussion. Concurrently, it underscores certain considerations when integrating these two approaches in the pedagogy of Marxist-Leninist philosophy
HIGHER EDUCATION AND ITS ENVIRONMENT IN MANIPUR
Authors: Ibempishak Devi Dr. Lukram
Published: May 2024

Many thinkers accept that Education is the learning centre or institute to bring up the students to be an ideal person .Education is what we want ? So teachers should be the construction of knowledge and co-learners of knowledge. Teachers take a great important role to make good society . Their important task is to make students to be an ideal person. So ,teacher is the soul or vital principle of educational system and no scheme of education can succeed without a competent body of teachers . The most genuine object of true education is the development of man as a human being with his own exigencies and sympathy with all other members of the human race. This is the bases which international society should develop . After independence many commission established with good suggestions by many educationist and recommended by the Central government but still now India cannot be included in the high literacy rate in the world . It might be having some deficiencies in the education sector of India . As Manipur also one of the seven sisters of North-East India ,the education of Manipur lies in the underdeveloped stage . The cause might be the negligence of both the State and the Central Government in the education sector . As education is the only means to develop human resources there is necessary to reform in the education system . The present education system is needy to modify both in the curriculum and the teaching methodology. The implementation and infrastructure is very low and insufficient . Hence, it is necessary to reform the education system of Manipur.
UNDERSTANDING THE CULTURE OF COMMUNICATION IN THE COLLEGE OF ARTS AND LETTERS OF BULACAN STATE UNIVERSITY-MAIN CAMPUS
Authors: G.Pineda Florentino , R. Buenaventura
Published: May 2024

Communication is the backbone of any academic institution. It enables collaboration, productivity, and innovation. This study explores the communication culture within the College of Arts and Letters (CAL) at Bulacan State University Campus. The goal is to understand the dynamics shaping communication practices, barriers, and strategies among administrators and faculty. Communication is crucial in educational settings, as it affects organizational unity, teaching quality, and student outcomes. Using communication theories and practices, the study uncovers the mechanisms driving communication interactions within CAL. Communication practices in modern educational settings are undergoing significant changes. As new technologies emerge and global competition increases, administrators and faculty face evolving challenges. This study examines communication within the CAL organization, exploring how administrators and faculty navigate these changes and use communication strategies to foster positive working relationships and improve instructional quality. The research takes a careful and thorough approach, using both numbers and stories to understand the communication culture within CAL. By surveying administrators and faculty, the study explores their views on communication practices, challenges, and strategies. Additionally, indepth interviews and group discussions provide valuable insights into the real-life experiences and perspectives of key people involved, deepening our understanding of the communication dynamics at play. The study reveals a diverse and interactive communication environment within CAL, marked by open discussions, information exchange, and collaborative problem-solving. However, the research also identifies common obstacles to effective communication, ranging from noise and poor feedback to insufficient communication channels and psychological barriers. Additionally, the study examines faculty perspectives on job performance, leadership capabilities, and communication dynamics with management, providing valuable insights into the relationship between individual attitudes and the organizational communication culture. Building on what we've learned, this study suggests ways to improve communication practices within CAL. From specialized training programs to optimizing communication channels and promoting a culture of open communication and collaboration, these recommendations provide a path to cultivating a cohesive, inclusive, and effective communication environment in the college. By exploring the communication culture at CAL, this research lays the foundation for nurturing a thriving academic community that supports learning, innovation, and growth.
COMPARATIVE STUDY OF COMMUNICATION DYNAMICS ACROSS DIFFERENT AGE GROUPS: INSIGHTS FROM IN-DEPTH INTERVIEWS AT TWO LEADING COMPANIES
Authors: Francine Eunice D.G Balala , De Guzman
Published: May 2024

This study focuses on the communication dynamics happening in the two institutions chosen by the researchers. The study aims to identify if the age differences of the workers of the respective institutions have an impact on how the employees and employers communicate with each other. The researchers selected two participants using a convenience sampling technique, one participant per institution. The use of semi-structured interviews as an instrument enabled to gathering of data and information needed for this research as a basis for assessing the research topic. The gathered data was then interpreted using thematic analysis. This approach ensured the credibility and relevance of the participants’ claims and enabled the researchers to explain further the results of the study. The study found out that the Baby Boomers working in Company 1, and the Millennials working in Company 2, have their own way of communicating inside the workplace. The study revealed that the two generations have their own communication preferences, communication processes and how they consider other elements in communicating, and conflicts in communication and its solutions. In line with this, the study suggests improvement and insights on how the institutions, and other organizations as well, to improve and maintain the communication dynamics between the workers inside their company. This study recommends further exploring the diversity of the different generations to better understand each and every one. Harmony of the people leads to the success of a company or organization.
ENHANCING COMMUNICATION DYNAMICS WITHIN ROCKAVILLA REALTY AND DEVELOPMENT CORPORATION: A STUDY BASED ON INTERVIEWS
Authors: Caparas  , Jefferson John
Published: May 2024

This study explores the communication dynamics among Civil Engineers employed at Rockavilla Realty and Development Corporation (RRDC). The study discovers the culture of communication and the challenges and flow of communication within the workplace. The researchers selected the participants through purposive sampling. This research paper utilizes a thematic analysis of transcripts from the semi-structured interview. The data collected from the 3 participants underwent analysis, coding, and interpretation, resulting in the identification of its themes. The result of the study showed that the communication culture's significant features at RRDC were positive attitude, flexible work schedule, and reliability. The findings also showed that the flow of communication at RRDC combines upward, downward, and horizontal communication. The feedback loop, which exists in negative and positive ways, was also determined. Researchers also conducted further investigations into the communication challenges faced in the field, which reveals conflict management and miscommunication as two of the challenges faced. This study's findings demonstrated the capability of civil engineer employees to communicate effectively despite recognizable and inevitable differences, which serve as indicators of success in work within the institution. The study discovered the significant features that unite every worker with varying cultural backgrounds, values, and beliefs, improving daily interaction and increasing productivity at the RRDC. Generally, this study offers essential insights into how Civil Engineers strive and prevail in the field, whether in construction or an office setting, through practical, dynamic, and inclusive communication where fundamental growth and skill improvement are also attained and assessed.
Dairesel Silindirlerin Girdap Kaynaklı Titreşimlerine Serbest Su Yüzeyi Etkisinin Deneysel ve Sayısal Olarak İncelenmesi
Authors: Duranay Aytekin
Published: May 2024

Girdap kaynaklı titreşimler (GKT), çeşitli yapı tiplerinde, açık deniz yapılarından durağan ve yüzen platformlara kadar, lineer olmayan bir akış–yapı etkileşimi fenomeni olarak karşımıza çıkmakta ve bu durum genellikle yapısal hasara ve istikrarsızlığa neden olmaktadır. Ancak, son yıllarda yapılan bazı GKT çalışmaları, fenomeni doğrudan yenilenebilir enerji üretimi için kullanmaya ya da çeşitli şekillerde yararlanmak amacıyla cihazlar geliştirmeye odaklanmıştır. Bu çalışmada, GKT’nin hem yıkıcı–tahrip edici özelliklerinden kaçınmaya hem de fenomenden yararlanmayı amaçlayan araştırmaların sıklıkla karşılaştığı ortak problemlerden biri olan serbest su yüzeyi etkisini yüksek Reynolds sayılarında inceleme amaçlanmıştır. Bu amaçla, serbest yüzeye yakın bir konumda bulunan dairesel bir silindirin GKT'si üzerine yapılan sayısal ve deneysel araştırmaların bulguları sunulmakta ve tartışılmaktadır. Çalışmada öncelikle, serbest su yüzeyinden yeterince uzakta konumlandırılan bir silindirin GKT performansı deneysel olarak verilmiştir. Ardından aynı silindir kademeli olarak yüzeye yaklaştırılmış ve sonunda sakin suda silindir üst noktası serbest su yüzeyi hizasında olacak şekilde konumlandırılmıştır. Serbest su yüzeyinden yeterince uzakta olduğu koşulda en yüksek genlik ürettiği kenetlenme durumundaki kütle oranı, yay sabiti, Reynolds sayısı gibi deneysel parametreler sabit tutularak sadece su içindeki derinliği değiştirilmiş ve her yeni koşul için hareketli silindir etrafındaki akış özelliklerine ve GKT performansına izahat getirilmiştir. Çalışma, serbest yüzey etkisi altında GKT hareketi gözlemlenen dairesel silindirin salınım genliğinin, frekansının ve faz farkının su içindeki derinliğine bağlı olarak kontrol edilebileceğini ve düzenlenebileceğini göstermektedir. Bu bulgular, açık denizde yerleştirilen rüzgâr türbinleri, dalga enerjisi üreteçleri, hidrojen ve güneş enerjisi tesisleri gibi birçok potansiyel uygulamanın daha güvenli ve uzun ömürlü olmasına sağlamasına katkı sağlayacak niteliktedir. Çalışmada, önerilen sayısal yöntem, parametrik analiz ve ana sonuçlar detaylı bir şekilde sunulmaktadır.
Пасивний дохід в Україні: джерела та перспективи
Authors: Карнаушенко А. С. , Петренко В. С. , Лопатньов Д. К.
Published: May 2024

Актуальність дослідження зумовлено тим, що Україна переживає складний період пов'язаний з економічними кризами, Covid-19 та війною, що прямо впливає на доходи громадян та змушує їх шукати альтернативні шляхи заробітку. Метою статті є систематизація та аналіз різноманітних джерел пасивного доходу, які доступні в Україні, з урахуванням їх потенційних переваг та недоліків, а також дослідження сучасних тенденцій розвитку пасивного доходу в українському контексті. Під час дослідження було використано аналітичний та систематичний підходи, а саме: проаналізовано різноманітні джерела інформації, включаючи наукові та публіцистичні матеріали, статистичні дані, звіти різних організацій щодо пасивного доходу для визначення основних джерел пасивного доходу та їх стану. В межах наукового дослідження було висунуто припущення, що розуміння різних джерел пасивного доходу та їх переваг допоможе українцям забезпечити стабільніше фінансове майбутнє. Адже розуміння та використання можливостей пасивного доходу може стати важливим інструментом для фінансової стабільності громадян. В дослідженні було використано наступні методи: метод  аналізу  та  синтезу,  систематизації  та  узагальнення; висновки дослідження було сформульовано за допомогою логічно-наслідкового методу. Автори дослідження акцентували увагу на різних джерелах пасивного доходу, проаналізували дані за попередні роки, визначили можливості використання різних джерел пасивного доходу у період війни та виклики, які стоять перед громадянам для збереження та примноження власних заощаджень. В результаті встановлено, що в Україні існує великий потенціал для отримання пасивного доходу, але його реалізація вимагає від громадян більш високого рівня фінансової грамотності, ніж він є зараз. Оригінальність та наукова новизна дослідження полягають у комплексному вивченні специфіки різних джерел пасивного доходу в контексті української економічної ситуації, включаючи їх переваги та обмеження, що дало змогу визначити структурний розподіл фізичних осіб в Україні, які отримували пасивний дохід станом на 31.12.2022 р. Перспективи подальших досліджень включатимуть глибше вивчення впливу пасивного доходу на економічний та соціокультурний розвиток України, а також проведення соціологічного опитування щодо намірів формування пасивного доходу серед молодого покоління. Стаття є теоретичною.
The Impact Of IRFS 16 Implementation On Tax Aggressiveness: Do Right-Of-Use Assets And Lease Liabilities Matter?
Authors: Sa’diyyah Dewi Khalimatus , Herwanda Reyhan , Firmansyah Amrie
Published: May 2024

Implementing PSAK 73 in Indonesia requires companies to disclose right-of-use assets and lease liabilities in their financial statements. This study examines the impact of changes in the disclosure of right-of-use assets and lease liabilities due to the implementation of PSAK 73 on tax aggressiveness. The research sample consisted of financial statements of companies in the consumer non-cyclical sector listed on the Indonesian Stock Exchange (IDX) from 2020 to 2022. Using purposive sampling, the study used 24 companies with 72 observations. The impact of the change was tested using panel data linear regression. The results showed that right-of-use assets have a positive impact on tax aggressiveness, while lease liabilities do not have a significant impact on tax aggressiveness. This study contributes to the literature on PSAK 73 and its relationship with tax aggressiveness. The Directorate General of Taxes can use the findings to develop tax monitoring and auditing strategies.
RESCUE ROBOT FOR BOREWELL
Authors:   Associate Professor ISE, The Oxford College of Engineering
Published: May 2024

Modern citification and development of cities has expanded since a decade, parchedness and deficient supply of water from conventional water reservoir and supply system has led to expand in number of domestic borewell installation. And price of installation and financing is easy and economical hence increasingly people are selecting for their personal bore well, rather relying of City Corporation for water supply. This has acutely enlarged the accident in the site of bore well especially toddlers are assailable and numerous incidents has transpired where toddlers smitten in open borewell and some falling into the depth. The intension of the project is to establish and design a bore well rescue robot, which can lift and rescue the kids who fall into well. Key words- rescue robot for borewell, confined space rescue, remote-controlled robotics, live streaming surveillance, humanitarian technology, cost-effective rescue system
Facial Emotion Recognition using Convolutional Neural Network
Authors: Sadat Mohammed Nashat
Published: May 2024

Emotion is an expression that human use in expressing their feelings. It can be express through facial expression, body language and voice tone. Humans' facial expression is a major way in conveying emotion since it is the most powerful, natural and universal signal to express humans' emotion condition. However, humans' facial expression has similar patterns, and it is very confusing in recognizing the expression using naked eye. For instance, afraid and surprised is very similar to one another. Thus, this will lead to confusion in determining the facial expression. Hence, this study aims to develop a application for emotion recognition that can recognize emotion based on facial expression in real-time. The Deep Learning based technique, Convolutional Neural Network (CNN) is implemented in this study. The Mobile Net algorithm is deployed to train the model for recognition. There are four types of facial expressions to be recognized which are happy, sad, surprise, and disgusting. Keywords: Facial Emotion Recognition, Deep Learning, CNN, Image Processing.
THE INFLUENCE OF ONLINE PRESENCE ON THE INTENSITY OF EMPLOYEE DISCIPLINE AT PT. PEGADAIAN (PERSERO) DAYA MAKASSAR BRANCH
Authors: Anisa Apriliani Malik  , Sri Andayaningsih  , A.Muhiddin Daweng
Published: May 2024

This research aims to determine the effect of Online Presence on Employee Discipline Intensity at PT. Pegadaian (Persero) Daya Makassar Branch. The population in this study were all employees of PT. Pegadaian (Persero) Daya Makassar Branch has 35 employees. The type of research is descriptive quantitative. The data sources used are primary data sources, namely information originating from observations and questionnaires. Meanwhile, secondary data sources are data sources obtained from documentation or literature study. The data analysis technique used is simple linear regression analysis using statistical calculations by Statistical Package for the Social Science (SPSS) version 24 application.The results of the research show that Online Presence (X) has a positive and significant effect on employee discipline intensity (Y) seen from the results of the calculated t value > t table (4,163 > 1.690) and the significant value 0.00 ˂ 0.05
EFFECT OF PROBIOTIC TREATMENT ON THE MICROBIOLOGICAL ACTIVITY OF UKRAINIAN TYPICAL BLACK SOIL
Authors: Pysarenko Pavlo , Samoilik Maryna , Taranenko Anna , Taranenko Serhiy , Bybyk Yevhen
Published: May 2024

The search for novel substances that promote the establishment of a microbial community and facilitate optimal humification processes while increasing soil organic matter content offers an opportunity for land restoration. The objective of study was to determine the abundance of ecological and trophic groups of soil microorganisms and the intensity of microbiological processes when subjected to probiotic treatment at various concentrations and doses in typical black soil. The eco-trophic groups of soil microorganisms were identified by inoculating dilutions of soil suspensions onto selective nutrient media. The direction and intensity of soil microbiological processes were assessed using the mineralization-immobilization, oligotrophy, and pedotrophy indexes. The results demonstrate that probiotics has a positive impact on the microbiological activity of the soil, leading to an increase in the number of ecological and trophic groups of soil microorganisms during both spring and autumn seasons. Notably, a significant effect on the soil microorganism conditions was observed after 30 days of probiotic treatment. Probiotics exhibit a favorable influence on microbiological processes within the soil, fostering conducive conditions for the development of soil microorganisms and the formation of humus. The most effective concentration of probiotics for promoting the functionality of microbial communities in black soils is determined to be 10%, accompanied by a dose of 100 l ha-1. Consequently, the application of probiotic treatment at a concentration of 10% and a dose of 100 l/ha-1 holds the potential to enhance the biological state of the soil, restore soil microbial diversity, and serve as an environmentally safe fertilizer.
DIAZOTROPHIC ENDOPHYTE Klebsiella sp. N5 FROM SORGHUM SHOWS CROSS-COLONIZATION AND PLANT GROWTH PROMOTION IN RICE
Authors: Gauswami Ashvin , JHA SANJAY , Dr. Swati Patel  , SUTHAR HARISH , Parekh Vipul , Jha Anamika , Jajda Harsur
Published: May 2024

Rice necessitates the highest levels of N fertilizers during its cultivation increasing demand for nitrogen fertilizers as well as negatively affecting the environment. Endophytic diazotrophic bacteria were isolated from sorghum, out of 35 isolates  Klebsiella sp. N5 showed maximum potential for biological nitrogen fixation. Primary screening of strain N5 was done with acetylene reduction assay indicating dry root activity of 638.55 nmol C2H4 h-1 g-1. That was further confirmed by nifH gene amplification. The strain was identified morphologically, biochemically and 16S rDNA sequencing analysis and identified as Klebsiella sp. strain N5. This strain exhibited other plant growth-promoting (PGP) traits, such as phosphate solubilization, production of indole acetic acid, siderophore activity, ACC deaminase activity, and anti-fungal activity. PGP endophyte N5 was inoculated into rice seedlings to investigate its interaction under axenic conditions and to characterize its ability to colonize non-native host plant rice. Extensive colonization of rice roots was corroborated by Scanning electron microscopy. In-planta experiments with rice seeds inoculated with Klebsiella sp. N5 showed significant improvements in various agronomic traits such as increase in shoot length, root length, fresh and dry weight of root and shoot, chlorophyll content, and nitrogen content of the plant and soil compared to the untreated plants. Klebsiella sp. N5, a cross colonizer from sorghum (C4 plant) to rice (C3 plant) plays a crucial role in facilitating the acquisition of essential resources such as nitrogen, phosphorus, and iron through direct mechanisms. This study expands the horizon for its potential as a valuable bioinoculant for sustainable agricultural practices, particularly in non-native host rice, paving the way for future field trials.
FUNCTIONING OF MICROORGANISMS IN THE RHIZOSPHERE OF PLANTS
Authors: Kurdish Ivan , Chobotarov Andrii
Published: May 2024

The functioning of living organisms in nature is possible only due to active interaction with microbiota. It is typical for phytobionts, the microbial cenosis of which is even more diverse. Plants contribute to the spread of microorganisms in the rhizosphere by releasing root exudates. The chemical composition of these exudates varies in different plant species and depends on the significant number of factors that affect their growth and development. The components of the plant root exudates stimulate expansion in the rhizosphere of microorganisms, useful for the plant growth. This microbiota improves the mineral life of plants considerably, stimulating their growth with biologically active compounds, increasing the availability of a number of microelements for them, protecting the growth of plants from phytopathogens and phytophages. It provides significant stimulation for the growth and development of phytobionts. It has been shown that microbial groups in the rhizosphere can induce some changes in the composition of plant root exudates.
DETERMINATION OF BIOGENIC AMINES IN CULTIVATED AND PROCESSED MUSHROOMS INTENDED FOR THE SLOVAK MARKET
Authors: Jakabová Silvia , Árvay Július , Grófová Magdaléna , Golian Jozef
Published: May 2024

Cultivated and processed white button mushrooms are widely used in the menu of the Slovak consumers. However, these food items can potentially contain biogenic amines, attributed to the presence of precursor compounds and their susceptibility to microbial spoilage. Biogenic amines significantly influence food quality and can pose health risks to consumers. This study is focused on determination of spermidine, putrescine, tyramine, cadaverine, histamine, spermine, and 2-phenylethylamine in processed mushroom products available in the Slovak market. The findings revealed considerable variability in biogenic amine content across individual products, influenced by the producer. Spermidine, putrescine and spermine emerged as the predominant biogenic amines with concentration ranges 2349.8 -7412.8, 37.2 - 607.5 and 74.2 - 267.6 mg/kg DW, respectively. Tyramine and histamine were detected in 22.2% of processed mushroom samples with one product exceeding concentration 200 mg/kg DW. Cadaverine was identified in 44.4% samples. The presence of histamine has to be concerned regarding potential health risks for consumers. Comparison of the mushroom products showed significant differences between the groups of products with whole mushrooms vs. sliced mushrooms in brine in case of the content of spermine (p<0.001), water content (p<0.01), putrescine, histamine and tyramine (p<0.05 for all these BAs). No significant differences were found between the groups for the cadaverine, spermidine and total content of biogenic amines. In house rapid validation was performed in term of determination of limits of detection and limits of quantification and intraday precision of retention time for individual biogenic amines in this study. Limits of detection ranged from 0.013 to 0.029 µg/mL and limits for quantification were in the range from 0.039 to 0.087 µg/mL. The RSD for the retention time in standard solutions did not exceed 0.12%.
QUALITY ASSESSMENT OF FERMENTED MEAT PRODUCT AFTER NATURAL EXTRACTS OF BLACKCURRANT (RIBES NIGRUM) AND KAMCHATKA HONEYSUCKLE (LONICERA CAERULEA VAR. KAMTSCHATICA) ADDITION
Authors: Kročko Miroslav , Hanuska Adam , Bobko Marek , Jurčaga Lukáš , Mesárošová Andrea
Published: May 2024

The aim of this work was to analyse the effect of blackcurrant (Ribes nigrum) and kamchatka honeysuckle (Lonicera caerulea var. Kamtschatica) extracts on the physico-chemical properties, microbiological and sensory quality of fermented meat product. Analyses were performed after 5, 15, 30 and 45 days of ageing. The addition of blackcurrant and kamchatka honeysuckle extracts in quantities of 3 and 5 ml.kg-¹ did not adversely affect the ageing process of fermented meat product samples determined based on pH. After 45 days of ageing period, we found higher malondialdehyde (MDA) values in all experimental groups (except for the experimental group with the addition of 3 ml blackcurrant extract). The addition of the extracts in the tested quantities did not cause demonstrable differences in the intensity values of brightness (L*), red (a*) and yellow (b*) colour throughout the ageing period. Microbial examination indicates that the addition of blackcurrant and kamchatka honeysuckle extracts did not inhibit the growth of starter culture. Bacteria of the genus Lactobacillus and Staphylococcus predominated in all experimental groups. Family Enterobacteriaceae was not detected after 45 days of ageing and storage. Moulds were detected only after 5 days of ageing and in the experimental groups with the addition of blackcurrant extracts. The counts of yeasts gradually increased with increasing ageing time in all experimental groups. Sensory analysis revealed a beneficial effect of kamchatka honeysuckle extract in the amount of 3 ml.kg-1 after 30 days of fermented sausages ageing. On the other hand, blackcurrant extract in higher amount (5 ml.kg-1) had a beneficial effect on sensory quality after 30th day. Traditional kamchatka honeysuckle aroma was probably transferred into the samples of meat product and caused lower sensory scores. Due to the known functional properties of black currant and kamchatka honeysuckle, we recommend carrying out their further analyses on the quality of fermented meat products.
PYOCIN S5 ACTIVITY AGAINST PSEUDOMONAS SYRINGAE PHYTOPATHOGENIC STRAINS
Authors: Balko Oleksandr , Zelena Lyubov , Balko Olha , Avdeeva Liliya
Published: May 2024

Pseudomonas syringae is the most frequently isolated bacterial plant pathogens. Previously we revealed a number of Pseudomonas aeruginosa strains – producers of bacteriocins (pyocins) with high activity against P. syringae. However, it was unknown, which type of pyocins possesses the activity against P. syringae strains. S5 and S1 pyocin genes were observed in the genome of most active strain–producer P. aeruginosa UCM В-333. Pyocins were induced by nalidixic acid (100 μg/ml). Using DEAE-cellulose chromatography we isolated two bacteriocin groups from initial lysate. Substances from the 10th fraction had nuclease activity (pyocin S1) and the 27th fraction - pore-forming activity (pyocin S5). It was revealed that S1 pyocin didn’t lead to growth inhibition of studied bacterial plant pathogens. However pyocin S5 caused appearance of growth inhibition area with diameter 14-20 mm. So, pyocins of S1 and S5 types are active substances in P. aeruginosa UCM В-333 lysates, but only S5 pyocin possess activity against P. syringae strains. fpt A receptor gene was revealed in genomes of all analyzed cultures, including used phytopathogenic strains. This fact can explain wide spectrum of pyocin S5 activity. It is suggested that pyocin S5 can be a potential biocontrol agent against phytopathogenic P. syringae strains.
Toezicht op zijn Brabants
Authors: de Bree M.A. , Meerman P.
Published: May 2024

Toezicht op zijn Brabants. Innovatief toezichthouden onder de Omgevingswet

De Omgevingswet, ingevoerd op 1 januari 2024, vraagt om vernieuwend toezichthouden. Dit artikel bespreekt de uitdagingen voor toezichthouders, zoals het veranderen van een controle- naar een oplossingsgerichte aanpak. In Noord-Brabant hebben omgevingsdiensten, veiligheidsregio’s en waterschappen een innovatieve methode ontwikkeld. Deze stelt de kwaliteit van de leefomgeving centraal en maakt gebruik van maatwerk, zelfevaluaties door bedrijven en een focus op interne borging. De aanpak bevordert samenwerking tussen overheid en bedrijven, stimuleert tweede-orde-leren, en biedt voorspellende waarde door vroegtijdige interventie.
The Economical and Geographical Importance of Lapis Route
Authors: Samoon Mohammad Zaman , Ahmadi Noor Ahmad
Published: May 2024

This research explores the intricate web of the Lapis Route, an ancient trade route that formerly linked Afghanistan with the Mediterranean Sea. This study examines the geographical and historical relevance of this ancient road using a qualitative research approach, building on insights from numerous books, journals, and other sources. It focuses on the valuable lapis lazuli gemstone that contributed to the route's notoriety.  The research delves into the various segments of the Lapis Route and its convergence with the Silk Road, unveiling the complex web of commerce that thrived amongst various societies. It reveals the significance of the Silk Road historically and emphasizes how it shaped both economic development and cross-cultural exchange.  In addition, the study looks into the economic importance of silk, a highly prized item that traveled the Silk Road and supported the region's economy. The study concludes by examining the historical, cultural, and economic significance of the Silk Road and the Lapis Route in Afghanistan.
The report addresses the opportunities and problems related to transit and commercial development, emphasizing the possible advantages of reviving the Silk Road. It highlights the possibilities that the revival of this historic trade route holds for fostering regional collaboration and economic progress.  This study provides important insights into the historical, geographical, and economic relevance of the Silk Road and the Lapis Route by analyzing them. It draws attention to the routes' lasting impact and their potential to support Afghanistan's and the region's future economic growth.
The Potential of Leukocyte Ratio to Differ Pediatric Tuberculosis from Bronchopneumonia
Authors: Benita Krist Nathania , Wulandari Asih Retno , Purwaningtyas Retno
Published: May 2024

Introduction: Differentiating between TB and pneumonia is crucial to avoid treatment delays. Delayed diagnosis can result in reduced patient survival rates, increased treatment expenses and prolonged treatment durations. Several leukocyte ratios such as Neutrophil-lymphocyte ratio (NLR), lymphocyte-monocyte ratio (LMR), platelet-lymphocyte ratio (PLR), monocyte-lymphocyte ratio (MLR) will be compared to confirm whether it can be used to distinguished pulmonary tuberculosis from community-acquired pneumonia.
Material and Methods: Retrospective study of 100 children aged 1-12 years who were treated at RSUD Dr. Haryoto Lumajang, period 1 January 2019 – 1 January 2023. Data on age, gender, diagnosis and laboratory results were obtained from medical records.
Results: Of the 100 children included 50 had TB disease and 50 had pneumonia. The mean of neutrophil counts was significantly higher in the control group compared to the case group. NLR and MLR ratio were statistically significant. NLR were both statistically significant in both <5 years old and >5 years old, but MLR were not significantly different in both age groups. A significant but weak positive correlation was found between tuberculosis (case) group, pneumonia (control) group, and age of children (r=0.132; p=0.048). Younger children (less than 5 years old) had 0.571-fold odds for tuberculosis infection compared to the older children.
Conclusion: NLR hold promise as readily accessible diagnostic biomarkers for distinguishing children with TB disease from those with pneumonia.
Assessing The Knowledge, Attitude and Practices of Malaria Prevention and Treatment in Mansa District, Luapula Province, Zambia
Authors: Lillian Mambwe  Mutesu  , Rodrick Sakamba  , Stanley Nyirenda  , Kingford Chimfwembe
Published: May 2024

Introduction: Malaria continues to be a major public health problem in Zambia with more than 16 million people at risk and 2015 estimates show that there were over 5 million malaria cases. Children under five years and pregnant women are at the greatest risk. Despite Zambia’s interventions including universal insecticide-treated bed-net (ITN) coverage and indoor residual spraying (IRS) in targeted areas strides in the fight against malaria, the situation in Luapula Province is totally different with the highest incidence in pregnancy, 131/1000 in 2013, 207/1000 in 2014, and 177/1000 in 2015. This research therefore assessed the knowledge, attitudes, and practices of malaria prevention and treatment in Mansa, Luapula province of Zambia.
Material and Methods: A descriptive cross-sectional study design adopting both qualitative and quantitative study approaches was used on 270 study participants who included community members, pregnant women and health personnel in Mansa ward of Mansa district. The participants were sampled using simple random and purposive sampling techniques. Data was collected using questionnaires, interview guides, and focus group discussions. The quantitative data was analyzed using SPSS statistical software, while the qualitative was thematically analyzed.
Results: Out of the 270 participants, knowledge of malaria transmission being due to mosquito vector was very high and seen in 270 (100%), however, other perceptions of transmission were; drinking contaminated water 3(1.1%), eating a lot of mangoes 7(2.6%) and coming in contact with a sick patient 207(76.7%). Participants negative attitudes stood at 196 (72.6%) and bad practices was 153(56.7%).  Education level was strongly associated with knowledge (p=0.000), attitudes (p=0.001) and practices (p=0.004).
Conclusion: Even though knowledge levels on malaria were high and comparable to other studies, however, some attitudes and practices were negative among the participants. On the contrary, healthcare providers had the right attitude towards malaria prevention and control. Community Health Workers are very important in the fight against malaria. The study recommends that creating health promotion messages taking into account the illiterates, involvement of traditional leaders, and giving incentives to community health workers can help in the fight against malaria in Luapula Province.
Natural farming negatively influences the growth of Sangyod Muang Phatthalung rice (Oryza sativa L.) but not its grain production or quality in preliminary comparison to conventional farming
Authors: Srisawat Theera , Tarasuk Tanapa , Kaosuwan Siriwan , Chimpud Warapong , Chumkaew Parinuch , Samala Sainiya , Sukolrat Apinya
Published: May 2024

Sangyod Muang Phatthalung (SMP) rice is a great source of various nutritional ingredients, but it is only grown in chemical-dependent systems with synthetic fertilizers and insecticides. Farming practices for food security and environmental conservation in the paddy field are highly controversial, but most practices have focused on either chemical-dependent or organic farming. In this study, we compared the growth, yield, quality, phytochemical profiles, and data on grain elements (EDX associated with SEM analysis) of SMP rice along with its production costs when using natural farming (NF) [Azolla and leaf compost applied] with those when using conventional farming (CF) [chemical fertilizer use (18-8-8 and 46-0-0) and high dose fertilizer (HF) as recommended by the supplier (18-4-5, 20-8-20, and 15-15-15)]. Our data show negative results in terms of plant growth and positive results in terms of yield, physical grain qualities, chemical grain qualities, and chemical composition for SMP rice grown under a natural farming system. A total of six important inorganic elements were present in the kernel (C and O) and the aleurone layer (P, K, Mg, and S) of rice grain. This study claims a high performance for natural farming, based on rice yield and quality with decreased production costs and a possible increase in the market price of high-value rice. These findings have significant implications for applying natural farming practices in the context of food security and environmental conservation, without risk to farmers’ finances and health or the agroecosystem.
Imprevisão Legal da Socioafetividade: Contribuições das Decisões Judiciais para o avanço do tema no Direito das Famílias
Authors: Cavalini Laura Toledo , Santos Guilherme Augusto Martins
Published: May 2024

O artigo examina o fenômeno de como as decisões judiciais no domínio do Direito de Família contribuem para mitigar o impacto da falta de normas jurídicas específicas em relação à socioafetividade. Inicia-se com uma análise da evolução da abordagem do direito de família em relação à socioafetividade ao longo do tempo. Em seguida, destaca-se o surgimento do princípio da afetividade como um valor jurídico fundamental. O estudo revela o significativo efeito das decisões judiciais na legitimação da socioafetividade no âmbito familiar, destacando a importância dessas decisões para preencher lacunas legais e promover a justiça social. A metodologia adotada envolve a revisão de decisões judiciais relevantes que reconhecem a socioafetividade em casos familiares, investigando seu impacto e implicações. Além disso, são identificadas as principais teorias e argumentos jurídicos utilizados pelos tribunais para fundamentar suas decisões relacionadas à socioafetividade na ausência de leis específicas. Conclui-se que as decisões judiciais desempenham um papel crucial no reconhecimento e na proteção dos vínculos socioafetivos nas relações familiares, evidenciando a necessidade de uma abordagem flexível e sensível do direito das famílias para acompanhar as transformações sociais e garantir a efetivação dos princípios constitucionais, como a dignidade da pessoa humana e a afetividade.