bluuebunny/crossref_metadata_2025 huggingface dataset:
PVA-Based MMMs for Ethanol Dehydration via Pervaporation: A Comparison Study between Graphene and Graphene Oxide
Authors: Zhan Xia , Ge Rui , Gao Zhongyong , Gao Teng , Wang Luying , Li Jiding
Published: January 2022

Two different types of 2D nanosheets, including hydrophobic graphene (GR) and hydrophilic graphene oxide (GO), were filled into poly (vinyl alcohol) (PVA) polymers to prepare mixed matrix membranes (MMMs) for ethanol dehydration via pervaporation. The relationship between the physical/chemical properties of graphene and pervaporation performance of MMMs was investigated by a comparison of GR/PVA and GO/PVA MMMs in microstructure and PV performance. The incorporation of GO nanosheets into PVA reduced PVA crystallinity and enhanced the membrane hydrophilicity, while the incorporation of GR into PVA led to the opposite results. The incorporation of GR/GO into PVA depressed the PVA membrane swelling degree, and the incorporation of GR showed a more obvious depression effect. GR/PVA MMMs showed a much higher separation factor than GO/PVA MMMs, while they exhibited a much lower permeation flux than GO/PVA MMMs and pristine PVA membranes. The huge difference in microstructure and performance between GO/PVA and GR/PVA MMMs was strongly associated with the oxygen-containing groups on graphene lamellae. The higher permeation flux of GO/PVA MMMs was ascribed to the facilitated transport of water molecules induced by oxygen-containing groups and exclusive channels provided by GO lamellae, while the much lower permeation flux and higher separation factor GR/PVA MMMs was resulted from the smaller GR interplanar spacing (0.33 nm) and hydrophobicity as well as barrier effect of GR lamellae on the sorption and diffusion of water molecules. It was presumed that graphene intercalated with an appropriate number of oxygen-containing groups might be a good choice to prepare PVA-based MMMs for ethanol dehydration, which would combine the advantages of GR’s high interlayer diffusion selectivity and GO’s high permeation properties. The investigation might open a door to achieve both of high permeation flux and separation factor of PVA-based MMMs by tuning the microstructure of graphene.
Flavonoids Extraction Kinetics, Antimicrobial Activity and Radical Scavenging Potential of Bulgarian Woundwort (Solidago virgaurea L.)
Authors: Yaneva Zvezdelina , Simeonov Evgeni , Rusenova Nikolina , Ivanova Donika , Nikolova Galina , Karamalakova Yanka , Chilev Chavdar , Beev Georgi
Published: January 2022

The medicinal plant woundwort (Solidago virgaurea L.) characterizes by diuretic, antimutagenic, anti-inflammatory activity and it has been applied for urinary tract, nephrolithiasis and prostate disorders treatment. The aim of the present study was to analyze the extraction kinetics of catechin, epigallocatechin and quercetin from Bulgarian woundwort extracts, to assess the antibacterial potential of the medicinal plant extracts against four bacterial strains (Staphylococcus aureus ATCC25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Bacillus cereus), their antioxidant capacity and radical scavenging potential. The concentrations of the flavonoids in the extracts obtained at different extraction conditions (solvent, temperature, extraction time) were determined by newly-developed by the scientific team RP-HPLC-PDA methodologies. The agar well diffusion method was applied to evaluate the antibacterial activity of the plant extracts. The 70% EtOH extracts at 20 °C displayed significantly higher antibacterial activity against the foodborne pathogenic bacteria S. aureus and P. aeruginosa as compared to the 70% and 98% EtOH extracts at 30 °C and 20 °C, respectively. The medicinal plant exhibited satisfactory antioxidant potential and radical-scavenging activity.
Fabrication of Poly(amide-co-ester) Solvent Resistant Nanofiltration Membrane from P-nitrophenol and Trimethyl Chloride via Interfacial Polymerization
Authors: Zhou Ayang , Li Lin , Li Mengying , Chen Qi
Published: January 2022

P-nitrophenol (PNP), a refractory hazardous substance, has not been efficiently utilized so far. In this paper, PNP is used as a membrane modification material for preparing poly(amide-co-ester) composite nanofiltration membrane. An organic solvent nanofiltration (OSN) membrane was prepared via interfacial polymerization reactionby using PNP and trimethyl chloride (TMC) on a ethylenediamine (EDA) crosslinked polyetherimide substrate. The results of ATR-FTIR and XPS show that interfacial polymerization occurs among with PNP and TMC and the terminal amine groups on the ethylenediamine crosslinked -PEI support forming a poly(amide-co-ester) toplayer. The NF-1PNP membrane maintained stable DMF performance permeance of 2.2 L m−2 h−1 bar−1 and rejection of 98% for Rose Bengal red (RB 1017.64 g mol−1) in 36 h continuous separation process. Furthermore, the average pore diameter of the two membranes including NF-1PNP and NF-1.25PNP, which is 0.40 and 0.36nm, respectively. This study not only provides a good way for the preparation of OSN membrane, but also provides a good demonstration for the comprehensive utilization of PNP and other toxic and harmful pollutants.
Impact of Producer’s Environmental Performance on Consumers and Retailers Simultaneously in the Indonesian Retail Environment
Authors: Fajar Muhamad Ibnu , Kusnoputranto Haryoto , Koestoer Raldi Hendro T. S. , Gozan Misri
Published: January 2022

Producers improve environmental performance to reduce the release of post-production waste. The positive environmental performance of producers is expected to influence consumer environmental attitudes positively, specifically toward post-consumption waste. However, research is deficient in exploring the impact of producer environmental performance (PEP) on retailer-consumer relationship quality (RCRQ) and consumer purchasing behavior (CPB) simultaneously to drive consumer environmental attitude (CEA) in the retail environment. Therefore, this study aims to examine the effect of PEP on RCRQ and CPB simultaneously to drive CEA in the retail environment. The survey was conducted at 17 retail outlets for 150 consumers of lubricant producers in the industrial city of Gresik, Indonesia. Partial Least Square analysis indicates that consumers perceive PEP has positive effects on CEA, but negatively affects RCRQ and positively affects CPB. Thereby, CPB has a more significant positive effect than RCRQ on CEA. Although RCRQ and CPB have significant positive total effects, CPB has a more significant indirect effect than RCRQ in mediating the effect of PEP on CEA. This study provides actionable guidance to reduce the negative impact of PEP on RCRQ to enhance the mediating role of RCRQ for more positive consumer environmental attitudes.
Impact of COVID-19 Pandemic on Daily Lives, Agricultural Working Lives, and Mental Health of Farmers in Northern Thailand
Authors: Sapbamrer Ratana , Chittrakul Jiraporn , Sirikul Wachiranun , Kitro Amornphat , Chaiut Wilawan , Panya Pattarika , Amput Patchareeya , Chaipin Eakasit , Sutalangka Chatchada , Sidthilaw Suttinee , Promrak Pornthip , Kamolsan Pailinrak , Hongsibsong Surat
Published: January 2022

This study aims to determine the impact of COVID-19 on the daily lives, agricultural working lives, and mental health of farmers in northern Thailand. This cross-sectional study was carried out in September and October 2021 by interviews. From the stratified random sampling, 2046 farmers responded. There were five sections on the interview form, including demographics, daily life, agricultural working life, stress, and depression experienced during the COVID-19 pandemic. The results show that COVID-19 negatively affected the daily lives of the farmers, making it worse by 61.2%. COVID-19 increased the cost of planting (57.4%) and the cost of agrochemicals and fertilizers (69.9%). It also decreased the prices of agricultural products (73.5%) as well as agricultural extensions (66.5%). The markets and logistics of agricultural products during the pandemic were more difficult than before it (72.8% and 65.1%, respectively). Half of the farmers (50.3%) had moderate stress, and the highest scores were for the loss of household income (mean ± SD = 3.92 ± 0.94) and increased household expenses (mean ± SD = 3.92 ± 0.98). With regard to depression, 19.6% of farmers had depressive symptoms, and the multivariate analysis shows that the mental health of farmers was associated with the changes in their daily and agricultural working lives, as well as with financial problems. The remarkable findings indicate that the farmers who had high and extremely high stress levels had a higher prevalence of depression than the farmers who had no stress (adj.OR = 10.10 and 22.45, respectively). Our results lead to the conclusion that the COVID-19 crisis had an impact on the daily lives, agricultural working lives, and mental health of farmers. The results of this study can be used to provide pertinent guidance, and they have implications for government and other relevant organizations in their COVID-19 efforts to improve agricultural systems and sustain the mental health of farmers.
Understanding Current Challenges in Evaluating Environmental Impacts for Aggregate Producers through a Case Study in Western Sweden
Authors: Lee Christina , Papadopoulou Panagiota , Asbjörnsson Gauti , Hulthén Erik , Evertsson Magnus
Published: January 2022

To improve environmental performance of a product or activity, an understanding of the environmental impacts associated with it is needed. Quantification of environmental impacts can be achieved through the standardized measurement-based tool of Life Cycle Assessment (LCA). However, challenges occur when trying to apply a standardized tool to a nonstandardized industry such as the aggregate industry. This study aims to provide greater understanding of the challenges facing the aggregate industry, particularly producers, in applying LCA. This was conducted through a literature review, to establish the current understanding of challenges, accompanied by a case study where a site-specific LCA was conducted with a large enterprise at a crushed-rock-production facility in western Sweden, to gain new industry-specific insight. A total of 13 challenges were identified: seven methodological and six systemic. Out of these 13 challenges, 3 were deemed a high risk to the implementation of LCA by aggregate producers, and 3 to the integrity of results. A best-practice framework is suggested to incorporate LCA into current environmental management techniques utilized at quarry sites in Sweden to overcome some challenges. However, LCA used for environmental management should not lead to double work if LCA is being utilized for Environmental Product Declarations, and further research is encouraged to find appropriate solutions with the most efficient allocation of the resources needed in conducting LCA studies.
Evaluation of Thermochemical Characteristics and Pyrolysis of Fish Processing Waste for Renewable Energy Feedstock
Authors: Reza Md Sumon , Azad Abul K. , Abu Bakar Muhammad S. , Karim Md Rezaul , Sharifpur Mohsen , Taweekun Juntakan
Published: January 2022

The necessity of energy is continuously increasing, whereas fossil fuel sources are gradually depleting. To mitigate this problem, fish processing waste of the bluespotted stingray (Neotrygon kuhlii), available in the Borneo region, was investigated for an alternative feedstock of bioenergy production. The fish wastes are hazardous for the environment, whereas the biodiesel from fish waste is pollution-free and produces less contaminant gas and carbon dioxide than fossil fuel. From the proximate analysis, the moisture content, volatile matter, fixed carbon, and ash content of the fish waste were achieved as 4.88%, 63.80%, 15.03%, and 16.29%, respectively. The proportion of carbon, hydrogen, nitrogen, sulfur, and oxygen was found as 42.06%, 5.99%, 10.77%, 0.91%, and 40.27%, respectively, from the ultimate analysis. The calorific value was 21.53 MJ/kg, which would be highly effective in biofuel production. The morphology analysis results of the biomass are favorable for renewable energy sources. The major bondage between carbon and hydrogen and oxygen was found using Fourier transform infrared spectroscopy. The thermogravimetric analysis and derivative thermogravimetry revealed that the highest weight loss occurred at 352 °C temperature with a decomposition rate of 4.57 wt.%/min in pyrolysis circumstances, and at 606 °C temperature with a decomposition rate of 3.77 wt.%/min in combustion conditions. In the pyrolysis process for 25 °C/min heating rate, the yield of biochar, bio-oil, and bio-syngas was found as 33.96, 29.34, 23.46% at 400 °C, 47.72, 49.32, 33.87% at 500 °C, and 18.32, 21.34, 42.37% at 600 °C, respectively. The characteristics and pyrolysis yields of fish waste are suitable for being an effective renewable energy source.
Integrated Policy Package Assessment (IPPA): A Problem-Oriented Research Approach for Sustainability Transformations
Authors: Scheer Dirk , Schmidt Maike , Dreyer Marion , Schmieder Lisa , Arnold Annika
Published: January 2022

In this paper, we present the Integrated Policy Package Assessment (IPPA) approach and relate IPPA to three substantial concepts of problem-oriented research concerned with societal transformations: technology assessment (TA), sustainability research (SR), and responsible research and innovation (RRI). The IPPA approach provides (political) decision-makers with transformation and orientation knowledge via a four-step process of (1) design, (2) analysis, (3) evaluation, and (4) discourse of a policy package assessment. It is illustrated with a case study of urban passenger transport. As an integrated approach, IPPA has substantial ties to TA, SR, and RRI. It connects with TA in fundamental ways, since it combines the field of TA with the field of regulatory assessment based on consequence analysis. Connectable to the field of SR, IPPA addresses deliberation processes and sustainable pathway identification based on multi-criteria assessment. In addition, akin to the area of RRI, IPPA shows cross-cutting axes with regards to social resonance assessment and stakeholder evaluation with a focus on multi-actor responsibilities. In this contribution, we link evidence-based impact assessment with transformation pathway mechanisms and corresponding policy packages, backed by stakeholder-based responsible innovation feedback loops. This enhances the ex-ante analysis of policy packages regarding their intended as well as unintended consequences.
Measurement of Innovation Efficiency in China’s Electronics and Communication Equipment Manufacturing Industry-Based on Dynamic Network SBM Model
Authors: Li Jinfa , Qin Ruijie , Jiang Hongbing
Published: January 2022

In recent years, China’s electronics and communication equipment manufacturing (ECEM) industry has overgrown, and the government should assess the innovation performance of the industry for its sustainable development. However, most previous studies on the innovation efficiency of the ECEM industry have ignored the link and carry-over variables. This paper uses the number of patent applications as a link variable to consider the stage of innovation activities. It divides the innovation activities of the electronics industry into two stages: technology development and results in transformation. To consider the dynamics of innovation activities, this paper uses capital stock as a period carry-over variable and evaluates the change of innovation efficiency over time. In this paper, the DNSBM model is used to measure the innovation efficiency of the ECEM industry in 26 Chinese provinces from 2013–2019. This model includes both stage link variables and period carry-over variables, thus allowing for overall efficiency and stage efficiency and period efficiency. The results show that the overall innovation efficiency values in the Chinese ECEM industry are low, there are considerable differences between the two-stage efficiency values in the east, central and western regions, and the overall efficiency values show a slow upward trend.
Residents’ Perception of the Impact of Sports Tourism on Sustainable Social Development
Authors: González-García Rómulo Jacobo , Mártínez-Rico Gabriel , Bañuls-Lapuerta Francesc , Calabuig Ferran
Published: January 2022

The analysis of the social perception of citizens and evaluations of the impact of sport tourism and their support for tourism development can be of great use in the formulation of policies aimed at social cohesion and local development. These actions favor social participation and inclusion, equal opportunities, and more positive attitudes towards sports tourism and inclusive sport. This study aims to analyze the perception and predisposition towards the support of citizens on the impact of sport tourism from a multidimensional perspective. Specifically, this study evaluates the social perception on four measures: social impact, cultural impact, environmental impact, and local policies. The sample of this study is composed of 607 people living in Gran Canaria. A survey with two scales were used, one on the perception of the social, cultural, environmental, economic, and political–administrative impact and the other was on the predisposition to support the development of sport tourism from the perspective of sustainable and inclusive social development. Using the partial least square methodology, the results show that all the study variables were significant except for social impact. It is concluded that there is a favorable social perception towards sport tourism. The results of this study might have a effect on the planning of the tourism sector in Gran Canaria as well as on the policies and regulations governing this activity.
The Role of a Hazardous Waste Intermediate Management Plant in the Circularity of Products
Authors: Viruega Sevilla David , Francisco López Ahinara , Bello Bugallo Pastora
Published: January 2022

Zero-pollution goals and the reduction in environmental pressures related to production and consumption have become a priority in recent environmental policies such as the 8th European Environment Action Program proposal. Adapting current industrial processes is essential to this transition towards a regenerative economy. This work presents a redesign plan for an industrial system that includes mechanical workshops and a hazardous waste intermediate management plant, covering all management activities (both off-site and on-site), such as collection, transport, and treatment. The waste management hierarchy is modified/amplified considering the original definition and the circular economy focus. This includes the improvement of existing processes and/or the design of new sustainable processes from waste to energy and useful materials, with different foci (integrated pollution prevention and control, industrial ecology, the circular economy, system dynamics, and life-cycle thinking (LCT)) and different tools employed (Best Available Techniques inventory (BAT), process simulation, BAT analysis, industrial symbiosis, dynamic material and energy flow analysis, and LCT tools). These tools help us to improve the sustainability of waste to energy and useful materials processes and improve symbiotic behaviour in the industrial system. This study shows the real possibility of achieving the circularity of products, transforming the waste sector into a productive one. Meanwhile, it contributes to the extinction of the traditional concept of waste.
Sustainability Reporting and Corporate Reputation: The Moderating Effect of CEO Opportunistic Behavior
Authors: Zimon Grzegorz , Arianpoor Arash , Salehi Mahdi
Published: January 2022

The present study’s main objective is to assess the impact of non-financial sustainability reporting (NFSR) on corporate reputation and the role of the CEO in the opportunistic behavior of companies listed on the Tehran Stock Exchange. In total, 178 firms were assessed for this paper during 2013–2020. In this study for calculating the NFSR, environmental sustainability reporting (ESR), social sustainability reporting (SSR), governance sustainability reporting (GSR) and ethical sustainability reporting (ETSR), Arianpoor and Salehi’s comprehensive and conceptual model has been used. In addition, the literature states that a CEO’s power can be classified as an opportunity for discretion and opportunistic behavior in CEOs that is in contrast with stakeholder demands. To this end, in this study, CEOs’ power has been used as an indicator for the CEO’s opportunistic behavior, and the CEO pay slice (CPS) index was used to calculate the CEO’s level of power. The results revealed that NFSR affects corporate reputation positively. In addition, ESR, SSR, ETSR and GSR positively affect corporate reputation. Moreover, the CEO’s power affects the relationship between NFSR/ESR/SSR/ETSR and corporate reputation. Because managers desire to engage in social and ethical activities, they try to hide the company’s errors and increase its reputation. The results revealed that the CEO’s power did not affect the relationship between GSR and corporate reputation. Since companies in the Tehran Stock Exchange are under intensive supervision, such as in governance, the impact of a CEO’s power and the interaction of a CEO’s power and GSR on company reputation in this study might, thus, not apply to these companies. It is crucial to investigate NFSR, corporate reputation and CEO power within Iran-specific conditions because of differences in emerging markets and developing countries such as Iran, which have diverse ownership structures, economic status, legal systems, government policies, and culture.
Red Mud as a Secondary Resource of Low-Grade Iron: A Global Perspective
Authors: Khanna Rita , Konyukhov Yuri , Zinoveev Dmitry , Jayasankar Kalidoss , Burmistrov Igor , Kravchenko Maksim , Mukherjee Partha S.
Published: January 2022

Managing red mud (RM), a solid waste byproduct of the alumina recovery process, is a serious ecological and environmental issue. With ~150 million tons/year of RM being generated globally, nearly 4.6 billion tons of RM are presently stored in vast waste reserves. RM can be a valuable resource of metals, minor elements, and rare earth elements. The suitability of RM as a low-grade iron resource was assessed in this study. The utilization of RM as a material resource in several commercial, industrial operations was briefly reviewed. Key features of iron recovery techniques, such as magnetic separation, carbothermal reduction, smelting reduction, acid leaching, and hydrothermal techniques were presented. RMs from different parts of the globe including India, China, Greece, Italy, France, and Russia were examined for their iron recovery potential. Data on RM composition, iron recovery, techniques, and yields was presented. The composition range of RMs examined were: Fe2O3: 28.3–63.2 wt.%; Al2O3: 6.9–26.53 wt.%; SiO2: 2.3–22.0 wt.%; Na2O: 0.27–13.44 wt.%; CaO: 0.26–23.8 wt.%; Al2O3/SiO2: 0.3–4.6. Even with a high alumina content and high Al2O3/SiO2 ratios, it was possible to recover iron in all cases, showing the significant potential of RM as a secondary resource of low-grade iron.
Characteristics of Body Posture in the Sagittal Plane in 8–13-Year-Old Male Athletes Practicing Soccer
Authors: Barczyk-Pawelec Katarzyna , Rubajczyk Krystian , Stefańska Małgorzata , Pawik Łukasz , Dziubek Wioletta
Published: January 2022

Background: An important part of a healthy lifestyle for children and adolescents is exercising to satisfy the natural need for physical activity. However, young athletes should take special care when they participate in intense physical training, to ensure their proper physical development. The aim of this study was to evaluate the body posture in the sagittal plane of soccer players in comparison with healthy untrained peers. Methods: A total of 245 young males aged 8–13 who participated in the study were divided into two groups: “Group F—Footballer”, elite youth soccer players comprising 132 male athletes, and “Group C—Control group”, consisting of 113 boys from primary schools. The elite, youth soccer players played and trained in the Gold Standard Certificate Academy for their age group and belonged to the top 1% of all players from their category, respectively (Polish Soccer Association (PZPN)). The control group consisted of healthy boys from primary schools in Wroclaw not practicing any sport. A photogrammetric method based on the projection moiré phenomenon was used to assess the body posture in all subjects. Results: The analysis showed statistically significant differences in body posture parameters in the sagittal plane between the trained (F) and non-trained (C) groups. In all age groups, a significantly higher value of the upper thoracic angle and a lower value of the trunk inclination angle were observed in the football players’ group. Significant differences were also observed for the thoracic spine in each age group. In the groups of 8–9 and 12–13 years, they concerned the angle of thoracic kyphosis, and in the group of 10–11-year-old, the depth of thoracic kyphosis. In the group of children with ages 8–9 and 10–11, significantly higher values of lumbosacral angle and upper thoracic angle were observed in the group of soccer players. Conclusions: The soccer training load can influence the anterior–posterior curvature of the spine. In the group of footballers in all age groups, higher angular values of thoracic kyphosis and greater tilt of the torso forward were found, compared with their untrained peers. There were also significant differences in body posture between children of different ages, both in the group of footballers and in the group of untrained children. In the group of footballers, the differences concerned mainly the size of the lumbosacral angle and the depth of both curves, which decreased with the age of the players. A similar phenomenon was observed in the group of untrained boys but only in children in the older age groups.
Vertical Shear Processes in River Plumes: Instabilities and Turbulent Mixing
Authors: Ayouche Adam , Carton Xavier , Charria Guillaume
Published: January 2022

In this paper, the problem of vertical shear flow instabilities at the base of a river plume and their consequences in terms of turbulent energy production and mixing is addressed. This study was carried out using 2D non-hydrostatic simulations and a linear stability analysis. The initial conditions used in these simulations were similar to those observed in river plumes near estuaries. Unstable stratified sheared flows follow three stages of evolution: (i) the generation of billows induced by vertical shear instabilities, (ii) intensification, and (iii) elongation. The elongation of the generated billows is related to the strain intensity, which depends on the physical setting involved (velocity shear, stratification thickness, and bottom slope). Two vertical shear instabilities were found in our study: the Holmboe and Kelvin–Helmholtz instabilities. The Kelvin–Helmholtz instability has a smaller growth time and longer wavelengths; the Holmboe instability is characterized by a longer growth time and shorter wavelengths. The Kelvin–Helmholtz instability is intensified when the bottom is sloped and for large shears. The Holmboe instability is stronger when the stratification thickness is reduced compared to the shear thickness and when the bottom is sloped. For mixing, the flow can be: (i) pre-turbulent, (ii) quasi-turbulent, or (iii) turbulent. The pre-turbulent flow corresponds to more mass mixing than momentum mixing and to more Eddy Kinetic Energy dissipation than Eddy Available Potential Energy dissipation. Such a flow is encountered over a flat bottom whatever the initial shear is. The quasi-turbulent and turbulent flows are reached when the bottom is sloped and when the stratification thickness is reduced. Using turbulent mixing statistics (mixing coefficients, mixing efficiency, Eddy Kinetic Energy, and Eddy Available Potential Energy dissipation rates), we showed that, despite their slow growth, Holmboe instabilities contribute more efficiently to turbulent mixing than Kelvin–Helmholtz instabilities. Holmboe instabilities are the only source of turbulent mixing when sharp density gradients are observed (small buoyancy thickness experiment). Our simulations highlight the contribution of the Holmboe instability to turbulent mixing.
‘Back-and-Forth Stomach’ CT Imaging Findings of a Pathophysiologic Entity Causing Acute Gastric Volvulus
Authors: Láinez Ramos-Bossini Antonio Jesús , Ruiz Carazo Eduardo , Rabadán Caravaca María Dolores
Published: January 2022

Background: Gastric volvulus (GV) is a life-threatening emergency condition that prompts emergent surgical management. With the advent of high-resolution computed tomography (CT), the role of radiologists in its diagnosis has become essential. Although many cases of GV have been described in the literature, its pathophysiology is still poorly understood. In addition, there is substantial terminological confusion with associated entities such as paraesophageal hernia, upside-down stomach, organo-axial or chronic GV. Methods: We conducted a retrospective review of clinical, radiological findings and other relevant data for seven patients with previous radiological diagnoses of a large hiatus hernia who presented with acute GV to the emergency department of our institution. We report data on age, sex, medical history, clinical presentation, imaging, treatment and outcomes for each case. Results: The CT findings at acute presentation showed the antrum lying above the diaphragm and dilated fundus below the diaphragm. By comparing the position of the stomach at acute presentation with previous imaging examinations, we confirmed a hypothesis put forward by a few authors decades ago that re-herniation of the gastric fundus into the abdomen is a common pathophysiologic trigger leading to acute GV. This hypothesis has not been supported by modern imaging examinations. Conclusions: We have provided imaging evidence supporting that the pathophysiology of many GVs is based on caudal re-descent of hiatal hernia into the abdominal cavity. Given the terminological disparity used in the literature in this context, we believe it appropriate to introduce and extend the term ‘back-and-forth stomach’ to refer to this type of GV.
Effects of Zinc Oxide Nanoparticles on Model Systems of the Intestinal Barrier
Authors: Mittag Anna , Owesny Patricia , Hoera Christian , Kämpfe Alexander , Glei Michael
Published: January 2022

Zinc oxide nanoparticles (ZnO NP) are often used in the food sector, among others, because of their advantageous properties. As part of the human food chain, they are inevitably taken up orally. The debate on the toxicity of orally ingested ZnO NP continues due to incomplete data. Therefore, the aim of our study was to examine the effects of two differently sized ZnO NP (<50 nm and <100 nm primary particle size; 123–614 µmol/L) on two model systems of the intestinal barrier. Differentiated Caco-2 enterocytes were grown on Transwell inserts in monoculture and also in coculture with the mucus-producing goblet cell line HT29-MTX. Although no comprehensive mucus layer was detectable in the coculture, cellular zinc uptake was clearly lower after a 24-h treatment with ZnO NP than in monocultured cells. ZnO NP showed no influence on the permeability, metabolic activity, cytoskeleton and cell nuclei. The transepithelial electrical resistance was significantly increased in the coculture model after treatment with ≥307 µmol/L ZnO NP. Only small zinc amounts (0.07–0.65 µg/mL) reached the basolateral area. Our results reveal that the cells of an intact intestinal barrier interact with ZnO NP but do not suffer serious damage.
Celebrating 120 Years of Butantan Institute Contributions for Toxinology
Authors: Moura-da-Silva Ana M.
Published: January 2022

A hundred and twenty years ago, the Butantan Institute was founded by the Brazilian physician and scientist Vital Brazil, combining, in the same institution, medical research, and the transfer of results to society in the form of health products [...]
Citrinin Mycotoxin Contamination in Food and Feed: Impact on Agriculture, Human Health, and Detection and Management Strategies
Authors: Kamle Madhu , Mahato Dipendra Kumar , Gupta Akansha , Pandhi Shikha , Sharma Nitya , Sharma Bharti , Mishra Sadhna , Arora Shalini , Selvakumar Raman , Saurabh Vivek , Dhakane-Lad Jyoti , Kumar Manoj , Barua Sreejani , Kumar Arvind , Gamlath Shirani , Kumar Pradeep
Published: January 2022

Citrinin (CIT) is a mycotoxin produced by different species of Aspergillus, Penicillium, and Monascus. CIT can contaminate a wide range of foods and feeds at any time during the pre-harvest, harvest, and post-harvest stages. CIT can be usually found in beans, fruits, fruit and vegetable juices, herbs and spices, and dairy products, as well as red mold rice. CIT exerts nephrotoxic and genotoxic effects in both humans and animals, thereby raising concerns regarding the consumption of CIT-contaminated food and feed. Hence, to minimize the risk of CIT contamination in food and feed, understanding the incidence of CIT occurrence, its sources, and biosynthetic pathways could assist in the effective implementation of detection and mitigation measures. Therefore, this review aims to shed light on sources of CIT, its prevalence in food and feed, biosynthetic pathways, and genes involved, with a major focus on detection and management strategies to ensure the safety and security of food and feed.
The Role of Von Willebrand Factor in the Pathogenesis of Pulmonary Vascular Thrombosis in COVID-19
Authors: Babkina Anastasiya S. , Ostrova Irina V. , Yadgarov Mikhail Ya , Kuzovlev Artem N. , Grechko Andrey V. , Volkov Alexey V. , Golubev Arkady M.
Published: January 2022

The increased plasma levels of von Willebrand factor (VWF) in patients with COVID-19 was reported in many studies, and its correlation with disease severity and mortality suggest its important role in the pathogenesis of thrombosis in COVID-19. We performed histological and immunohistochemical studies of the lungs of 29 patients who died from COVID-19. We found a significant increase in the intensity of immunohistochemical reaction for VWF in the pulmonary vascular endothelium when the disease duration was more than 10 days. In the patients who had thrombotic complications, the VWF immunostaining in the pulmonary vascular endothelium was significantly more intense than in nonsurvivors without thrombotic complications. Duration of disease and thrombotic complications were found to be independent predictors of increased VWF immunostaining in the endothelium of pulmonary vessels. We also revealed that bacterial pneumonia was associated with increased VWF staining intensity in pulmonary arterial, arteriolar, and venular endothelium, while lung ventilation was an independent predictor of increased VWF immunostaining in arterial endothelium. The results of the study demonstrated an important role of endothelial VWF in the pathogenesis of thrombus formation in COVID-19.
Three Novel Bacteriophages, J5a, F16Ba, and z1a, Specific for Bacillus anthracis, Define a New Clade of Historical Wbeta Phage Relatives
Authors: Nakonieczna Aleksandra , Rutyna Paweł , Fedorowicz Magdalena , Kwiatek Magdalena , Mizak Lidia , Łobocka Małgorzata
Published: January 2022

Bacillus anthracis is a potent biowarfare agent, able to be highly lethal. The bacteria dwell in the soil of certain regions, as natural flora. Bacteriophages or their lytic enzymes, endolysins, may be an alternative for antibiotics and other antibacterials to fight this pathogen in infections and to minimize environmental contamination with anthrax endospores. Upon screening environmental samples from various regions in Poland, we isolated three new siphophages, J5a, F16Ba, and z1a, specific for B. anthracis. They represent new species related to historical anthrax phages Gamma, Cherry, and Fah, and to phage Wbeta of Wbetavirus genus. We show that the new phages and their closest relatives, phages Tavor_SA, Negev_SA, and Carmel_SA, form a separate clade of the Wbetavirus genus, designated as J5a clade. The most distinctive feature of J5a clade phages is their cell lysis module. While in the historical phages it encodes a canonical endolysin and a class III holin, in J5a clade phages it encodes an endolysin with a signal peptide and two putative holins. We present the basic characteristic of the isolated phages. Their comparative genomic analysis indicates that they encode two receptor-binding proteins, of which one may bind a sugar moiety of B. anthracis cell surface.
The Reservoir of Persistent Human Papillomavirus Infection; Strategies for Elimination Using Anti-Viral Therapies
Authors: Zheng Ke , Egawa Nagayasu , Shiraz Aslam , Katakuse Mayako , Okamura Maki , Griffin Heather M. , Doorbar John
Published: January 2022

Human Papillomaviruses have co-evolved with their human host, with each of the over 200 known HPV types infecting distinct epithelial niches to cause diverse disease pathologies. Despite the success of prophylactic vaccines in preventing high-risk HPV infection, the development of HPV anti-viral therapies has been hampered by the lack of enzymatic viral functions, and by difficulties in translating the results of in vitro experiments into clinically useful treatment regimes. In this review, we discuss recent advances in anti-HPV drug development, and highlight the importance of understanding persistent HPV infections for future anti-viral design. In the infected epithelial basal layer, HPV genomes are maintained at a very low copy number, with only limited viral gene expression; factors which allow them to hide from the host immune system. However, HPV gene expression confers an elevated proliferative potential, a delayed commitment to differentiation, and preferential persistence of the infected cell in the epithelial basal layer, when compared to their uninfected neighbours. To a large extent, this is driven by the viral E6 protein, which functions in the HPV life cycle as a modulator of epithelial homeostasis. By targeting HPV gene products involved in the maintenance of the viral reservoir, there appears to be new opportunities for the control or elimination of chronic HPV infections.
ViralFlow: A Versatile Automated Workflow for SARS-CoV-2 Genome Assembly, Lineage Assignment, Mutations and Intrahost Variant Detection
Authors: Dezordi Filipe Zimmer , Neto Antonio Marinho da Silva , Campos Túlio de Lima , Jeronimo Pedro Miguel Carneiro , Aksenen Cleber Furtado , Almeida Suzana Porto , Wallau Gabriel Luz ,   on behalf of the Fiocruz COVID-19 Genomic Surveillance Network
Published: January 2022

The COVID-19 pandemic is driven by Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) that emerged in 2019 and quickly spread worldwide. Genomic surveillance has become the gold standard methodology used to monitor and study this fast-spreading virus and its constantly emerging lineages. The current deluge of SARS-CoV-2 genomic data generated worldwide has put additional pressure on the urgent need for streamlined bioinformatics workflows. Here, we describe a workflow developed by our group to process and analyze large-scale SARS-CoV-2 Illumina amplicon sequencing data. This workflow automates all steps of SARS-CoV-2 reference-based genomic analysis: data processing, genome assembly, PANGO lineage assignment, mutation analysis and the screening of intrahost variants. The pipeline is capable of processing a batch of around 100 samples in less than half an hour on a personal laptop or in less than five minutes on a server with 50 threads. The workflow presented here is available through Docker or Singularity images, allowing for implementation on laptops for small-scale analyses or on high processing capacity servers or clusters. Moreover, the low requirements for memory and CPU cores and the standardized results provided by ViralFlow highlight it as a versatile tool for SARS-CoV-2 genomic analysis.
A Proposed Guideline for Performance of Emergency Surgical Airways in Small Animals: Analysis of Five Unsuccessfully Managed Cannot Intubate, Cannot Oxygenate Cases
Authors: Hardjo Sureiyan , Goodwin Wendy , Haworth Mark David , Purcell Sarah Leonie
Published: January 2022

Objective—To describe three dogs and two cats diagnosed with a cannot intubate, cannot oxygenate (CICO) condition, and discuss the appropriateness and timing of emergency front-of-neck airway access (eFONA). The authors aim to increase awareness of CICO events and effective management strategies, which may result in faster airway access and improve patient outcomes. Case series summary—Three dogs and two cats could not be easily intubated resulting in the inability to deliver oxygen and contributing to their death. Emergency front-of-neck airway access was attempted in three cases, it could not be performed in one when indicated, and it was not considered in managing another. Conclusions—This is the first report of small animals suffering CICO emergencies and tracheostomy attempts without a concurrently secured airway. Cannot intubate, cannot oxygenate events and eFONA attempts were managed sub-optimally in all cases, which likely contributed to the poor outcomes. Rapid diagnosis of CICO and early eFONA using appropriate techniques has the potential to improve the management of difficult airways in small animals.
Current Use of Equine Laparoscopy in Urogenital Disorders: A Scoping Review of the Literature from 2000 to 2021
Authors: Straticò Paola , Guerri Giulia , Palozzo Adriana , Varasano Vincenzo , Petrizzi Lucio
Published: January 2022

(1) Background: Laparoscopic surgery replaced traditional invasive techniques for the treatment of common urogenital disorders in equids. The aim of this review is to evaluate applications and the development of urogenital laparoscopy from 2001 to 2021. (2) Methods: A scoping review of literature was undertaken according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines for scoping reviews on three databases (NCBI-PubMed, Web of Science-Thompson Reuters, and SciVerse Scopus). (3) Results: A total of 452 papers were identified. After duplicate removal and title screening, 181 papers underwent abstract screening. Of these, 160 + 10 papers (cited by others) were assessed for eligibility according to the PICOs. A total of 132 papers were considered eligible. Most of the research was focused on ovaries and testes, followed by urinary bladder and general articles about laparoscopy in horses. We identified 43 original studies (33%, RCT, NoRCT, and experimental trials), 39 case series/retrospective studies (29%), 37 case reports (28%), and 13 reviews (10%, narrative or systematic). (4) Conclusions: Gonadal disorders were the most investigated. Hand-Assisted Laparoscopic Surgery (HALS) and laparoscopic-assisted surgery represent valuable options for more challenging conditions (uterine and urinary bladder disorders).
Systematic Review of the Longitudinal Sensitivity of Precision Tasks in Visual Working Memory
Authors: Ades James , Mishra Jyoti
Published: January 2022

Precision of working memory (WM) refers to the objective performance of individuals when trying to recall the features of the encoded WM items. Studies of precision in VWM aim to identify whether differences in WM performance within individuals are sensitive to individual states or traits. In this systematic review, we study VWM precision and whether it reflects true differences in ability to accurately store information, and thereby possibly a more sensitive measure than discrete VWM span alone. Sifting through 327 abstracts, we identified 34 relevant articles. After assessing these articles with regard to our inclusion criteria to test participants at two separate time points and have a sample size of at least fifteen participants, we found four longitudinal studies regarding VWM precision. One review author and two reviewers independently assessed all studies in the screening and selection process and extracted outcome measures, study characteristics, and, when possible, test–retest reliability metrics. Given the small and heterogeneous sample, this systematic review could not yet provide conclusive evidence on the sensitivity of VWM precision paradigms. Future research of VWM should include longitudinal studies of precision, and address both test–retest reliability in healthy adults and changes in precision during key developmental trajectory periods and in clinical populations.
Impacts of Land-Use Change, Associated Land-Use Area and Runoff on Watershed Sediment Yield: Implications from the Kaduna Watershed
Authors: Daramola Japheth , Adepehin Ekundayo J. , Ekhwan Toriman M. , Choy Lam K. , Mokhtar Jaafar , Tabiti Tabiti S.
Published: January 2022

An uncontrolled sediment influx from the watershed upstream is a known threat to dam stability, while the pattern and amount of sediment yield are influenced by the predominant upstream land-use and land cover (LULC) types, precipitation amount, and intensity. Hence, the need to monitor sediment yield accumulation and its controlling factors in dam operation becomes crucial. In this paper, the Soil and Water Assessment Tool (SWAT) was used to assess the roles of land-use change, land cover area, and runoff on watershed’s sediment yield based on change detection analysis between 1975 and 2013 in the Kaduna Watershed (Nigeria), Western Africa. The SWAT standard procedures for the simulation of hydrological characteristics and sediment yields prediction were adopted. The datasets were calibrated for a period of 46 years and validated using 2015–2017 measured flow data, and suspended sediments concentration (SSC) acquired between March and October 2018. The model function was statistically determined using the Nash-Sutcliffe (NS), the coefficient of determination (r2) and the percentage of observed data (p-factor). The evaluation results of the SWAT model yielded NS, r2 and p-factor of 0.71, 0.80, and 0.86, respectively. These data suggest that the model performed satisfactorily for streamflow and sediment yield predictions. Findings suggest that the extinction of evergreen forests and a significant change in land-use from range grasses and forest to agriculture generic and residential types between 1975 and 2013, which resulted in surface runoff, sediment yield, and flow alteration. Evapotranspiration increased by 22.40% between 1975 and 2013. These changes have negatively impacted the watershed runoff by 56.00% and model sediment yield by 68.00% at the end of 2013. Thus, these variations can influence various human activities in the watershed, such as food security, livestock, energy production and water supply. It is hypothesized from the presented data that land use types exact a more dominant control on runoff and sediment yield than land cover area, although climatic influence may not be ruled out.
Research on Micro-Quantitative Detection Technology of Simulated Waterbody COD Based on the Ozone Chemiluminescence Method
Authors: Li Peng , Wang Yizhuo , Xu Bo
Published: January 2022

Chemical oxygen demand (COD), reflecting the degree of waterbody contaminated by reduction substances, is an important parameter for water quality monitoring. The existing measurement method of waterbody COD takes time and is a complex system, which cannot meet the real-time monitoring requirements of river pollution indicators. We developed the vortex t-structure microfluidic detection chip with the help of microfluidic technology and designed the COD detection system with a high integration degree based on the principle of ozone chemiluminescence, and we have also carried out research on a waterbody COD quantitative detection test. The test results show that the detection chip can generate quantitative and controllable ozone-based bubbles; it also shows the advantages of a simple system and short test time without environmental pollution, which provides some technical support for the online real-time monitoring of river water quality.