bluuebunny/crossref_metadata_2025 huggingface dataset:
Data Quality: A Negotiator between Paper-Based and Digital Records in Pakistan’s TB Control Program
Authors: Ali Syed Mustafa , Naureen Farah , Noor Arif , Kamel Boulos Maged N. , Aamir Javariya , Ishaq Muhammad , Anjum Naveed , Ainsworth John , Rashid Aamna , Majidulla Arman , Fatima Irum
Published: July 2018

Background: The cornerstone of the public health function is to identify healthcare needs, to influence policy development, and to inform change in practice. Current data management practices with paper-based recording systems are prone to data quality defects. Increasingly, healthcare organizations are using technology for the efficient management of data. The aim of this study was to compare the data quality of digital records with the quality of the corresponding paper-based records using a data quality assessment framework. Methodology: We conducted a desk review of paper-based and digital records over the study duration from April 2016 to July 2016 at six enrolled tuberculosis (TB) clinics. We input all data fields of the patient treatment (TB01) card into a spreadsheet-based template to undertake a field-to-field comparison of the shared fields between TB01 and digital data. Findings: A total of 117 TB01 cards were prepared at six enrolled sites, whereas just 50% of the records (n = 59; 59 out of 117 TB01 cards) were digitized. There were 1239 comparable data fields, out of which 65% (n = 803) were correctly matched between paper based and digital records. However, 35% of the data fields (n = 436) had anomalies, either in paper-based records or in digital records. The calculated number of data quality issues per digital patient record was 1.9, whereas it was 2.1 issues per record for paper-based records. Based on the analysis of valid data quality issues, it was found that there were more data quality issues in paper-based records (n = 123) than in digital records (n = 110). Conclusion: There were fewer data quality issues in digital records as compared with the corresponding paper-based records of tuberculosis patients. Greater use of mobile data capture and continued data quality assessment can deliver more meaningful information for decision making.
Formulation of the Phasors of Apparent Harmonic Power: Application to Non-Sinusoidal Three-Phase Power Systems
Authors: Blasco Pedro A. , Montoya-Mira Rafael , Diez José M. , Montoya Rafael , Reig Miguel J.
Published: July 2018

In this work, the expression of the phasor of apparent power of harmonic distortion is formulated in the time domain. Applying this phasor along with the phasor of apparent unbalance power allows us to obtain a new set of phasors that include all of the inefficient power components appearing in the transfer of energy in non-linear and unbalanced systems. In this manner, a new model of inefficient power in electrical systems is developed. For each voltage harmonic of order ‘m’ and current harmonic of order ‘n’, a phasor of harmonic apparent power is obtained. Accuracy in the determination of the total apparent power of a system depends on the number of harmonics considered. Each phasor of apparent harmonic power is formed from six mutually orthogonal parameters or components that are calculated from the harmonic voltages at the nodes of the network and the circulating harmonic currents. To demonstrate the validity of the proposed formulation, a four-wire non-linear system formed by two nodes is assessed.
Using a Marginal Value Approach to Integrate Ecological and Economic Objectives across the Minnesota Landscape
Authors: De Pellegrin Llorente Irene , Hoganson Howard M. , Windmuller-Campione Marcella , Miller Steve
Published: July 2018

Forest management situations are intrinsically challenging due to the nature of being an interconnected and multi-faceted problem. Integrating ecological, social, and economic objectives is one of the biggest hurdles for forest planners. Often, decisions made with the interest of producing a specific ecosystem service may affect the production of other forest ecosystem services. We present a forest management scheduling model that involves multiple ownerships and addresses the joint production of two ecosystem services: timber and upland hardwood old forest. We use a marginal value approach to evaluate old forest. We analyze the impacts of considering different management options, shapes and levels of marginal value functions for old forest, and potential benefits of rewarding the major forest land ownership groups to produce old forest. Results show the downward-sloping marginal value function as a compromise strategy and the benefits of applying it over approaches using either fixed values or targets for addressing ecosystem services. A decomposition model was useful for recognizing important stand-level detail. A broad landscape and multiple ownership approach helped identify interconnections between forest cover types and between landowner groups.
Encapsulation of Citrus By-Product Extracts by Spray-Drying and Freeze-Drying Using Combinations of Maltodextrin with Soybean Protein and ι-Carrageenan
Authors: Papoutsis Konstantinos , Golding John B. , Vuong Quan , Pristijono Penta , Stathopoulos Costas E. , Scarlett Christopher J. , Bowyer Michael
Published: July 2018

The effect of different combinations of maltodextrin (MD) coating agents (MD, MD + soybean protein, and MD + ι-carrageenan) on the encapsulation of lemon by-product aqueous extracts using freeze-drying and spray-drying were investigated. The total phenolic content (TPC), total flavonoid content (TFC), and ferric ion reducing antioxidant power (FRAP) of the microparticles were evaluated. Freeze-drying with the mixture of MD + soybean protein resulted in the highest retention of TPC, TFC, and FRAP (1.66 ± 0.02 mg GAE/g d.b., 0.43 ± 0.02 mg CE/g d.b., and 3.70 ± 0.05 mM TE/g, respectively). Freeze-drying resulted in microparticles with lower moisture content (MC) and water activity (aw) than those produced by spray-drying. Specifically, the MC and aw of the microparticles produced by freeze-drying ranged from 1.15 to 2.15% and 0.13 to 0.14, respectively, while the MC and aw of the microparticles produced by spray-drying ranged from 6.06% to 6.60% and 0.33 to 0.40, respectively. Scanning electron microscopy revealed that spray-drying resulted in the formation of spherical particles of different sizes regardless of the type of coating agent. Although freeze-drying resulted in microparticles with amorphous glassy shapes, the mixture of MD + soybean protein resulted in the formation of spherical porous particles. X-ray diffraction revealed a low degree of crystallinity for the samples produced by both techniques.
Children Residing in Low-Income Households Like a Variety of Vegetables
Authors: Overcash Francine M. , Reicks Marla , Ritter Allison , Leak Tashara M. , Swenson Alison , Vickers Zata
Published: July 2018

Child vegetable intake falls far below the minimum recommended levels. Knowing which vegetables children may like help those responsible for providing vegetables to children to improve intake. The objective of this study was to measure vegetable liking for a wide variety of vegetables by a racially and ethnically diverse population of 9–12-year old children from low-income families. Children rated their liking of 35 vegetables using a 10-point hedonic scale. We tabulated the number of children that found each vegetable acceptable (ratings of ‘okay’ or above) and the number that found each vegetable unacceptable (ratings below ‘okay’). More than 50% of children who had tried a vegetable considered it acceptable. A large majority of the vegetables had mean ratings in the acceptable range. Corn was the most liked vegetable, closely followed by potatoes, lettuce, and carrots. Artichoke had the lowest mean liking, followed by onion and beets. We found children liked a wide variety of vegetables which offers counter evidence to the commonly held perception that children do not like vegetables.
GADD45B as a Prognostic and Predictive Biomarker in Stage II Colorectal Cancer
Authors: Zhao Zhixun , Gao Yibo , Guan Xu , Liu Zheng , Jiang Zheng , Liu Xiuyun , Lin Huixin , Yang Ming , Li Chunxiang , Yang Runkun , Zou Shuangmei , Wang Xishan
Published: July 2018

GADD45B acts as a member of the growth arrest DNA damage-inducible gene family, which has demonstrated to play critical roles in DNA damage repair, cell growth, and apoptosis. This study aimed to explore the potential relationship between GADD45B expression and tumor progression and evaluate the clinical value of GADD45B in stage II colorectal cancer (CRC). The expression patterns and prognostic value of GADD45B in CRC were analyzed based on The Cancer Genomic Atlas (TCGA). GADD45B expression features of 306 patients with stage II CRC and 201 patients with liver metastasis of CRC were investigated using immunochemical staining on tissue microarrays. Afterward, survival analysis and stratification analysis were performed in stage II to explore the prognostic and predictive significance of GADD45B. Overexpressed GADD45B is associated with poorer prognosis for CRC patients both in overall survival (OS) (p < 0.001) and disease-free survival (DFS) (p = 0.001) based on the TCGA database. Analysis results according to the stage II CRC cohort and the liver metastatic CRC cohort revealed that GADD45B was gradually upregulated in normal mucosa including primary colorectal cancer (PCC). Colorectal liver metastases (CLM) tissues were arranged in order (normal tissue vs. PCC p = 0.005 and PCC vs. CLM p = 0.001). The low GADD45B group had a significantly longer five-year OS (p = 0.001) and progression-free survival (PFS) (p < 0.001) than the high GADD45B group for the stage II patients. The multivariate Cox regression analysis results proved that the expression level of GADD45B was an independent prognostic factor for stage II after radical surgery (OS: Hazard Ratio (HR) 0.479, [95% confidence interval (CI) 0.305–0.753] and PFS:HR 0.490, [95% CI 0.336–0.714]). In high GADD45B expression subgroup of stage II cohort, the patients who underwent adjuvant chemotherapy had longer PFS than those who did not (p = 0.008). High expression levels of GADD45B is an independent prognostic factor of decreased OS and PFS in stage II CRC patients. The stage II CRC patients with high GADD45B expression might benefit from adjuvant chemotherapy.
Dual RNA-Seq Analysis of Trichophyton rubrum and HaCat Keratinocyte Co-Culture Highlights Important Genes for Fungal-Host Interaction
Authors: Petrucelli Monise , Peronni Kamila , Sanches Pablo , Komoto Tatiana , Matsuda Josie , Silva Wilson , Beleboni Rene , Martinez-Rossi Nilce , Marins Mozart , Fachin Ana
Published: July 2018

The dermatophyte Trichophyton rubrum is the major fungal pathogen of skin, hair, and nails that uses keratinized substrates as the primary nutrients during infection. Few strategies are available that permit a better understanding of the molecular mechanisms involved in the interaction of T. rubrum with the host because of the limitations of models mimicking this interaction. Dual RNA-seq is a powerful tool to unravel this complex interaction since it enables simultaneous evaluation of the transcriptome of two organisms. Using this technology in an in vitro model of co-culture, this study evaluated the transcriptional profile of genes involved in fungus-host interactions in 24 h. Our data demonstrated the induction of glyoxylate cycle genes, ERG6 and TERG_00916, which encodes a carboxylic acid transporter that may improve the assimilation of nutrients and fungal survival in the host. Furthermore, genes encoding keratinolytic proteases were also induced. In human keratinocytes (HaCat) cells, the SLC11A1, RNASE7, and CSF2 genes were induced and the products of these genes are known to have antimicrobial activity. In addition, the FLG and KRT1 genes involved in the epithelial barrier integrity were inhibited. This analysis showed the modulation of important genes involved in T. rubrum–host interaction, which could represent potential antifungal targets for the treatment of dermatophytoses.
Citizenship as Barrier and Opportunity for Ancient Greek and Modern Refugees
Authors: Gray Benjamin
Published: July 2018

Some dominant traditions in Refugee Studies have stressed the barrier which state citizenship presents to the displaced. Some have condemned citizenship altogether as a mechanism and ideology for excluding the weak (G. Agamben). Others have seen citizenship as an acute problem for displaced people in conditions, like those of the modern world, where the habitable world is comprehensively settled by states capable of defending their territory and organised in accordance with interstate norms, which leaves very limited space for the foundation of new communities with their own meaningful citizenship (H. Arendt). This paper engages with these prominent approaches, but also with more recent arguments that, when handled and adapted in the right way, the practices and ideology of citizenship also present opportunities for the displaced to form their own meaningful communities, exercise collective agency, and secure rights. It is argued that the evidence from ancient Greece shows that ancient Greek citizenship, an early forerunner of modern models of citizenship, could be imaginatively harnessed and adapted by displaced people and groups, in order to form effective and sometimes innovative political communities in exile, even after opportunities to found new city-states from scratch became quite rare (after c. 500 BC). Some relevant displaced groups experimented with more open and cosmopolitan styles of civic interaction and ideology in their improvised quasi-civic communities. The different kinds of ancient Greek informal ‘polis-in-exile’ can bring a new perspective on the wider debates and initiatives concerning refugee political agency and organisation in the ‘provocations’ in this special issue.
Soviet Central Asia and the Preservation of History
Authors: Benjamin Craig
Published: July 2018

Central Asia has one of the deepest and richest histories of any region on the planet. First settled some 6500 years ago by oasis-based farming communities, the deserts, steppe and mountains of Central Asia were subsequently home to many pastoral nomadic confederations, and also to large scale complex societies such as the Oxus Civilization and the Parthian and Kushan Empires. Central Asia also functioned as the major hub for trans-Eurasian trade and exchange networks during three distinct Silk Roads eras. Throughout much of the second millennium of the Common Era, then under the control of a succession of Turkic and Persian Islamic dynasties, already impressive trading cities such as Bukhara and Samarkand were further adorned with superb madrassas and mosques. Many of these suffered destruction at the hands of the Mongols in the 13th century, but Timur and his Timurid successors rebuilt the cities and added numerous impressive buildings during the late-14th and early-15th centuries. Further superb buildings were added to these cities by the Shaybanids during the 16th century, yet thereafter neglect by subsequent rulers, and the drying up of Silk Roads trade, meant that, by the mid-18th century when expansive Tsarist Russia began to incorporate these regions into its empire, many of the great pre- and post-Islamic buildings of Central Asia had fallen into ruin. This colonization of the region by the Russians, and its later incorporation into the Union of Society Socialist Republics in 1919, brought Central Asia to the attention of Russian and Soviet archaeologists and urban planners. It was these town planners and engineers who were eventually responsible for preserving many of the decaying monuments and historic urban cores of Central Asia, despite the often-challenging ideological constraints they were forced to work under. The paper focuses particularly on the effect of these preservation policy decisions in Uzbekistan, where the process has been best documented. It argues that Soviet authorities struggled constantly with ways of recognizing the need for historical preservation while at the same time creating a new society that had cast off the shackles of its ‘feudal past’.
Lyme Disease Transmission Risk: Seasonal Variation in the Built Environment
Authors: Roome Amanda , Spathis Rita , Hill Leah , Darcy John M. , Garruto Ralph M.
Published: July 2018

Seasonal variation in spatial distribution and pathogen prevalence of Borrelia burgdorferi in blacklegged ticks (Ixodes scapularis) influences human population risk of Lyme disease in peri-urban built environments. Parks, gardens, playgrounds, school campuses and neighborhoods represent a significant risk for Lyme disease transmission. From June 2012 through May 2014, ticks were collected using 1 m2 corduroy cloths dragged over low-lying vegetation parallel to walkways with high human foot traffic. DNA was extracted from ticks, purified and presence of B. burgdorferi assessed by polymerase chain reaction amplification. Summer is reported as the time of highest risk for Lyme disease transmission in the United States and our results indicate a higher tick density of 26.0/1000 m2 in summer vs. 0.2/1000 m2 to 10.5/1000 m2 in spring and fall. However, our findings suggest that tick infection rate is proportionally higher during the fall and spring than summer (30.0–54.7% in fall and 36.8–65.6% in spring vs. 20.0–28.2% in summer). Seasonal variation in infected tick density has significant implications for Lyme disease transmission as people are less likely to be aware of ticks in built environments, and unaware of increased infection in ticks in spring and fall. These factors may lead to more tick bites resulting in Lyme infection.
Simple Urea Immersion Enhanced Removal of Tetracycline from Water by Polystyrene Microspheres
Authors: Ma Junjun , Li Bing , Zhou Lincheng , Zhu Yin , Li Ji , Qiu Yong
Published: July 2018

Antibiotics pose potential ecological risks in the water environment, necessitating their effective removal by reliable technologies. Adsorption is a conventional process to remove such chemicals from water without byproducts. However, finding cheap adsorbents with satisfactory performance is still a challenge. In this study, polystyrene microspheres (PSM) were enhanced to adsorb tetracycline by surface modification. Simple urea immersion was used to prepare urea-immersed PSM (UPSM), of which surface groups were characterized by instruments to confirm the effect of immersion. Tetracycline hydrochloride (TC) and doxycycline (DC) were used as typical adsorbates. The adsorptive isotherms were interpreted by Langmuir, Freundlich, and Tempkin models. After urea immersion, the maximum adsorption capacity of UPSM at 293 K and pH 6.8 increased about 30% and 60%, achieving 460 mg/g for TC and 430 mg/g for DC. The kinetic data were fitted by first-order and second-order kinetics and Weber–Morris models. The first-order rate constant for TC adsorption on UPSM was 0.41 /h, and for DC was 0.33 /h. The cyclic urea immersion enabled multilayer adsorption, which increased the adsorption capacities of TC on UPSM by two to three times. The adsorption mechanism was possibly determined by the molecular interaction including π–π forces, cation-π bonding, and hydrogen bonding. The simple surface modification was helpful in enhancing the removal of antibiotics from wastewater with similar structures.
Job Satisfaction and Associated Factors among Medical Staff in Tertiary Public Hospitals: Results from a National Cross-Sectional Survey in China
Authors: Zhou Huixuan , Han Xueyan , Zhang Juan , Sun Jing , Hu Linlin , Hu Guangyu , Wu Shichao , Zhao Pengyu , Jiang Feng , Liu Yuanli
Published: July 2018

Medical staff in China’s tertiary public hospitals are responsible for providing healthcare to a considerable number of patients, and their job satisfaction needs attention. The aim of this study is to investigate the job satisfaction of medical staff in tertiary public hospitals and to explore its associated factors. Based on a national survey conducted in 2016, this study included 43,645 physicians and nurses nested in 136 tertiary public hospitals in 31 provinces of China. Multi-level logistic regression was used to examine job satisfaction and its association with individual characteristics and job-related factors. Results showed that 48.22% respondents were satisfied with their job, and they were least satisfied with their compensation. Individual characteristics including occupation, gender, education background, alcohol drinking and self-reported health status, as well as job-related factors regarding professional title, work years, income, workload, doctor-patient relationship and practice setting were found to be significantly associated with job satisfaction. Given that some of these factors may be amenable to interventions, we suggest that government and hospital administrators could take some measures to promote continuing education, improve personal health, balance workload and compensation for medical staff, in order to improve the job satisfaction of medical staff in tertiary public hospitals.
Analysis of the Relationship between the Referral and Evolution of Patients with Type 2 Diabetes Mellitus
Authors: Alvarez Camilo , Saint-Pierre Cecilia , Herskovic Valeria , Sepúlveda Marcos
Published: July 2018

Type 2 Diabetes Mellitus (T2DM) is a chronic disease that has risen in prominence in recent years and can cause serious complications. Several studies show that the level of adherence to different types of treatment has a direct correlation with the positive evolution of chronic diseases. While such studies relate to patient adherence to medication, those that concern adherence to medical appointments do not distinguish between the different disciplines that attend to or refer patients. This study analyses the relationship between adherence to referrals made by three distinct disciplines (doctors, nurses, and nutritionists) and the results of HbA1c tests from a sample of 2290 patients with T2DM. The aim is to determine whether a relationship exists between patient improvement and the frequency with which they attend scheduled appointments in a timely manner, having been previously referred from or to a particular discipline. Results showed that patients tended to be more adherent when their next appointment is with a doctor, and less adherent when it is with a nurse or nutritionist. Furthermore, patients that remained stable had higher rates of adherence, whereas those with lower adherence tended to be more decompensated. The results can enable healthcare professionals to monitor patients and place particular emphasis on those who do not attend their scheduled appointments in a timely manner.
Plant Metabolomics in the Global Scenario of Food Security: A Systems-Biology Approach for Sustainable Crop Production
Authors: Iriti Marcello , Vitalini Sara
Published: July 2018

n/a
Characterization of UDP-Activated Purinergic Receptor P2Y6 Involved in Japanese Flounder Paralichthys olivaceus Innate Immunity
Authors: Li Shuo , Li Jiafang , Wang Nan , Hao Gaixiang , Sun Jinsheng
Published: July 2018

Uridine 5’-diphosphate (UDP)-activated purinergic receptor P2Y6 is a member of a G-protein-coupled purinergic receptor family that plays an important role in mammalian innate immunity. However, the role of the P2Y6 receptor (P2Y6R) in fish immunity has not been investigated. In this report, we characterized a P2Y6R gene from Japanese flounder (Paralichthys olivaceus) and examined its role in fish innate immunity. Sequence analysis reveals that the Japanese flounder P2Y6R protein is conserved and possesses four potential glycosylation sites. Quantitative real-time RT-PCR analysis shows that P2Y6R is broadly distributed in all examined Japanese flounder tissues with dominant expression in the liver. In addition, P2Y6R gene expression was up-regulated in head kidney macrophages (HKMs) upon lipopolysaccharides (LPS) and poly(I:C) stimulations but down-regulated by LPS challenge in peripheral blood leukocytes (PBLs). Furthermore, pharmacological inhibition of the endogenous P2Y6 receptor activity by the potently selective P2Y6R antagonist, MRS 2578, greatly up-regulated pro-inflammatory cytokine interleukin (IL)-1β, IL-6 and TNF-α gene expression in PBL cells treated with UDP. Moreover, LPS- and poly(I:C)-induced gene expression of IL-1β and TNF-α in Japanese flounder PBL cells was attenuated significantly by inhibition of P2Y6R activity with antagonist MRS 2578. Collectively, we, for the first time, showed the involvement of functional purinergic P2Y6R in fish innate immunity.
Identification and In Silico Prediction of Anticoagulant Peptides from the Enzymatic Hydrolysates of Mytilus edulis Proteins
Authors: Qiao Meiling , Tu Maolin , Chen Hui , Mao Fengjiao , Yu Cuiping , Du Ming
Published: July 2018

Mytilus edulis is a typical marine bivalve mollusk. Many kinds of bioactive components with nutritional and pharmaceutical activities in Mytilus edulis were reported. In this study, eight different parts of Mytilus edulis tissues, i.e., the foot, byssus, pedal retractor muscle, mantle, gill, adductor muscle, viscera, and other parts, were separated and the proteins from these tissues were prepared. A total of 277 unique peptides from the hydrolysates of different proteins were identified by UPLC-Q-TOF-MS/MS, and the molecular weight distribution of the peptides in different tissues was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The bioactivity of the peptides was predicted through the Peptide Ranker database and molecular docking. Moreover, the peptides from the adductor muscle were chosen to do the active validation of anticoagulant activity. The active mechanism of three peptides from the adductor muscle, VQQELEDAEERADSAEGSLQK, RMEADIAAMQSDLDDALNGQR, and AAFLLGVNSNDLLK, were analyzed by Discovery Studio 2017, which also explained the anticoagulant activity of the hydrolysates of proteins from adductor muscle. This study optimized a screening and identification method of bioactive peptides from enzymatic hydrolysates of different tissues in Mytilus edulis.
In Vivo Performance of Decellularized Vascular Grafts: A Review Article
Authors: Lin Chih-Hsun , Hsia Kai , Ma Hsu , Lee Hsinyu , Lu Jen-Her
Published: July 2018

Due to poor vessel quality in patients with cardiovascular diseases, there has been an increased demand for small-diameter tissue-engineered blood vessels that can be used as replacement grafts in bypass surgery. Decellularization techniques to minimize cellular inflammation have been applied in tissue engineering research for the development of small-diameter vascular grafts. The biocompatibility of allogenic or xenogenic decellularized matrices has been evaluated in vitro and in vivo. Both short-term and long-term preclinical studies are crucial for evaluation of the in vivo performance of decellularized vascular grafts. This review offers insight into the various preclinical studies that have been performed using decellularized vascular grafts. Different strategies, such as surface-modified, recellularized, or hybrid vascular grafts, used to improve neoendothelialization and vascular wall remodeling, are also highlighted. This review provides information on the current status and the future development of decellularized vascular grafts.
Impact of Long-Term RF-EMF on Oxidative Stress and Neuroinflammation in Aging Brains of C57BL/6 Mice
Authors: Jeong Ye Ji , Son Yeonghoon , Han Na-Kyung , Choi Hyung-Do , Pack Jeong-Ki , Kim Nam , Lee Yun-Sil , Lee Hae-June
Published: July 2018

The expansion of mobile phone use has raised questions regarding the possible biological effects of radiofrequency electromagnetic field (RF-EMF) exposure on oxidative stress and brain inflammation. Despite accumulative exposure of humans to radiofrequency electromagnetic fields (RF-EMFs) from mobile phones, their long-term effects on oxidative stress and neuroinflammation in the aging brain have not been studied. In the present study, middle-aged C57BL/6 mice (aged 14 months) were exposed to 1950 MHz electromagnetic fields for 8 months (specific absorption rate (SAR) 5 W/kg, 2 h/day, 5 d/week). Compared with those in the young group, levels of protein (3-nitro-tyrosine) and lipid (4-hydroxy-2-nonenal) oxidative damage markers were significantly increased in the brains of aged mice. In addition, levels of markers for DNA damage (8-hydroxy-2′-deoxyguanosine, p53, p21, γH2AX, and Bax), apoptosis (cleaved caspase-3 and cleaved poly(ADP-ribose) polymerase 1 (PARP-1)), astrocyte (GFAP), and microglia (Iba-1) were significantly elevated in the brains of aged mice. However, long-term RF-EMF exposure did not change the levels of oxidative stress, DNA damage, apoptosis, astrocyte, or microglia markers in the aged mouse brains. Moreover, long-term RF-EMF exposure did not alter locomotor activity in aged mice. Therefore, these findings indicate that long-term exposure to RF-EMF did not influence age-induced oxidative stress or neuroinflammation in C57BL/6 mice.
BRCA1 Interacting Protein COBRA1 Facilitates Adaptation to Castrate-Resistant Growth Conditions
Authors: Yun Huiyoung , Bedolla Roble , Horning Aaron , Li Rong , Chiang Huai-Chin , Huang Tim-H , Reddick Robert , Olumi Aria F. , Ghosh Rita , Kumar Addanki P.
Published: July 2018

COBRA1 (co-factor of BRCA1) is one of the four subunits of the negative elongation factor originally identified as a BRCA1-interacting protein. Here, we provide first-time evidence for the oncogenic role of COBRA1 in prostate pathogenesis. COBRA1 is aberrantly expressed in prostate tumors. It positively influences androgen receptor (AR) target gene expression and promoter activity. Depletion of COBRA1 leads to decreased cell viability, proliferation, and anchorage-independent growth in prostate cancer cell lines. Conversely, overexpression of COBRA1 significantly increases cell viability, proliferation, and anchorage-independent growth over the higher basal levels. Remarkably, AR-positive androgen dependent (LNCaP) cells overexpressing COBRA1 survive under androgen-deprivation conditions. Remarkably, treatment of prostate cancer cells with well-studied antitumorigenic agent, 2-methoxyestradiol (2-ME2), caused significant DNA methylation changes in 3255 genes including COBRA1. Furthermore, treatment of prostate cancer cells with 2-ME2 downregulates COBRA1 and inhibition of prostate tumors in TRAMP (transgenic adenocarcinomas of mouse prostate) animals with 2-ME2 was also associated with decreased COBRA1 levels. These observations implicate a novel role for COBRA1 in progression to CRPC and suggest that COBRA1 downregulation has therapeutic potential.
A Study of a Health Resources Management Platform Integrating Neural Networks and DSS Telemedicine for Homecare Assistance
Authors: Massaro Alessandro , Maritati Vincenzo , Savino Nicola , Galiano Angelo , Convertini Daniele , De Fonte Emanuele , Di Muro Maurizio
Published: July 2018

The proposed paper is related to a case of study of an e-health telemedicine system oriented on homecare assistance and suitable for de-hospitalization processes. The proposed platform is able to transfer efficiently the patient analyses from home to a control room of a clinic, thus potentially reducing costs and providing high-quality assistance services. The goal is to propose an innovative resources management platform (RMP) integrating an innovative homecare decision support system (DSS) based on a multilayer perceptron (MLP) artificial neural network (ANN). The study is oriented in predictive diagnostics by proposing an RMP integrating a KNIME (Konstanz Information Miner) MLP-ANN workflow experimented on blood pressure systolic values. The workflow elaborates real data transmitted via the cloud by medical smart sensors and provides a prediction of the patient status. The innovative RMP-DSS is then structured to enable three main control levels. The first one is a real-time alerting condition triggered when real-time values exceed a threshold. The second one concerns preventative action based on the analysis of historical patient data, and the third one involves alerting due to patient status prediction. The proposed study combines the management of processes with DSS outputs, thus optimizing the homecare assistance activities.
Chiral, Heterometallic Lanthanide–Transition Metal Complexes by Design
Authors: Øwre Anders , Vinum Morten , Kern Michal , Van Slageren Joris , Bendix Jesper , Perfetti Mauro
Published: July 2018

Achieving control over coordination geometries in lanthanide complexes remains a challenge to the coordination chemist. This is particularly the case in the field of molecule-based magnetism, where barriers for magnetic relaxation processes as well as tunneling pathways are strongly influenced by the lanthanide coordination geometry. Addressing the challenge of design of 4f-element coordination environments, the ubiquitous Ln(hfac)3 moieties have been shown to be applicable as Lewis acids coordinating transition metal acetylacetonates facially leading to simple, chiral lanthanide–transition metal heterodinuclear complexes. The broad scope of this approach is illustrated by the synthesis of a range of such complexes LnM: LnM(hfac)3(μ2-acac-O,O,O′)3 (Ln = La, Pr, Gd; M = Cr, Fe, Ga), with approximate three-fold symmetry. The complexes have been crystallographically characterized and exhibit polymorphism for some combinations of 4f and 3d metal centers. However, an isostructural set of systems spanning several lanthanides which exhibit spontaneous resolution in the orthorhombic Sohncke space group P212121 is presented here. The electronic structure and ensuing magnetic properties have been studied by EPR spectroscopy and magnetometry. The GdFe, PrFe, and PrCr complexes exhibit ferromagnetic coupling, while GdCr exhibits antiferromagnetic coupling. GdGa exhibits slow relaxation of the magnetization in applied static fields.
Wasp Size and Prey Load in Cerceris fumipennis (Hymenoptera, Crabronidae): Implications for Biosurveillance of Pest Buprestidae
Authors: Nalepa Christine , Swink Whitney
Published: July 2018

The relationship between predator and prey size was studied in the buprestid hunting wasp Cerceris fumipennis Say in eight widely distributed nesting aggregations in North Carolina, USA. Initial work indicated a significant linear relationship between wasp head width and wasp wet weight; thus, head width was used to estimate wasp body mass in subsequent studies. Prey loads of hunting females was studied by measuring the head width of the wasp, then identifying and weighing the prey item brought back to the nest. There was significant variation in wasp size among nesting aggregations; the average estimated wasp body mass in one site was double that in another. Prey weight varied with wasp weight, but larger wasps had a slight tendency to carry proportionally larger prey. Beetles captured by large wasps (≥120 mg) were significantly more variable in weight than those taken by small wasps (<80 mg). All but the smallest wasps could carry more than their own body weight. Prey loads ranged from 4.8–150.2% of wasp weight. Evidence suggests that small wasps bring back more of the economically important buprestid genus Agrilus and thus would be most efficient in biosurveillance for pest buprestids.
Electronic Band Structure Variations in the Ceria Doped Zirconia: A First Principles Study
Authors: Ramin Gul Sahar , Khan Matiullah , Zeng Yi , Lin Maohua , Wu Bo , Tsai Chi-Tay
Published: July 2018

Using first principle calculations, the effect of Ce with different doping concentrations in the network of Zirconium dioxide (ZrO2) is studied. The ZrO2 cell volume linearly increases with the increasing Ce doping concentration. The intrinsic band gap of ZrO2 of 5.70 eV reduces to 4.67 eV with the 2.08% Ce doping. In 4.16% cerium doped ZrO2, the valence band maximum and conduction band minimum come closer to each other, about 1.1 eV, compared to ZrO2. The maximum band gap reduction of ZrO2 is observed at 6.25% Ce doping concentration, having the value of 4.38 eV. No considerable shift in the band structure is found with further increase in the doping level. The photo-response of the ZrO2 is modulated with Ce insertion, and two distinct modifications are observed in the absorption coefficient: an imaginary part of the dielectric function and conductivity. A 2.08% Ce-doped ZrO2 modeled system reduces the intensities of peaks in the optical spectra while keeping the peaks of intrinsic ZrO2. However, the intrinsic peaks related to ZrO2 completely vanish in 4.16%, 6.25%, 8.33%, and 12.5% Ce doped ZrO2, and a new absorption hump is created.
Impact of the Direct Ageing Procedure on the Age Hardening Response of Al-Mg-Si 6101 Alloy
Authors: Osuch Piotr , Walkowicz Monika , Knych Tadeusz , Dymek Stanislaw
Published: July 2018

Al-Mg-Si alloys are used not only as construction material, but also as a material for electrical conductors. For this application, it is crucial for the alloy to achieve a balance between strength and electrical properties. This is achieved in practice by a combination of strain and precipitation hardening. The current paper focuses on a heat treatment procedure in which the EN AW 6101 alloy is cooled by a flowing air stream from the solutionizing temperature down to the artificial ageing temperature. The proposed procedure, unlike the common heat treatment leading to the T6 temper, allowed for the precipitation of the coarser β” phase with the presence of relatively wide precipitate-free zones. The age hardening response was investigated by Brinell hardness measurements, eddy current testing and microstructural observations using transmission electron microscopy (TEM). The applied heat treatment resulted in slightly lower strength (compared to the T6 temper), but improved electrical performance of the alloy.
Synthesis and Properties of Nanosized Stoichiometric Cobalt Ferrite Spinel
Authors: Bartůněk Vilém , Sedmidubský David , Huber Štěpán , Švecová Marie , Ulbrich Pavel , Jankovský Ondřej
Published: July 2018

Nanoparticles with controllable sizes of ferrite spinel CoFe2O4 were formed by thermal treatment of cobalt-iron glycerolate. Thermal behavior during the heating was studied by differential thermal analysis combined with thermogravimetry. The precursor, as well as the prepared nanoparticles, were analyzed by a broad spectrum of analytic techniques (X-Ray photoelectron spectroscopy (XPS), X-Ray diffraction (XRD), Energy dispersive spectroscopy (EDS), Atomic absorption spectroscopy (AAS), Scanning electron microscopy (SEM), and Raman spectroscopy). The particle size of nanoparticles was obtained from Transmission electron microscopy and also calculated using Scherrer formula. A vibrating sample magnetometer (VSM) in a Physical Property Measurement System was used to analyze the magnetic properties of nanoparticles.
Characterization of Microstructural Refinement and Hardness Profile Resulting from Friction Stir Processing of 6061-T6 Aluminum Alloy Extrusions
Authors: Netto Nelson , Tiryakioğlu Murat , Eason Paul D.
Published: July 2018

In this study, the change in microstructure and microhardness adjacent to the tool during the friction stir processing (FSP) of 6061-T6 extrusions was investigated. Results showed that the as-received extrusions contained Fe-rich constituent particles with two distinct size distributions: coarse particles in bands and finer particles in the matrix. After FSP, Fe-containing particles exhibited single-size distribution and the coarse particles appeared to be completely eliminated through refinement. Microhardness tests showed the presence of four distinct zones and that hardness increased progressively from the dynamically recrystallized closest to the tool, outward through two distinct zones to the base material. The similarities and differences between the results of this study and others in the literature are discussed in detail.
A Simple and Efficient Method for Obtaining the Whole-Range Uniaxial Tensile Properties of Pipeline Steel
Authors: Kong Lingzhen , Su Lingbo , Zhou Xiayi , Chen Liqiong , Chen Jie , Mao Cheng
Published: July 2018

To obtain the whole-range true stress-true strain curves of API X65, a method is proposed based on the equal proportion principle and digital images. The tensile elongation was obtained by tracing the gauge points on the specimen surface, and the true strain and true stress of API X65 were calculated according to the formulae. The obtained true stress-true strain curves were validated by a 3-D finite element model. The true stress-true strain curve was set as the input data, while the engineering stress-engineering strain curve was set as the output data. The output data of the finite element model was the same as that of the experiment test. The findings imply that the proposed method could acquire reliable, whole-range true stress-true stain curves. These curves, which depict the material behavior of pipeline steel from initial elongation to fracture, could provide basic data for pipeline defect tolerance limit analysis and fracture assessment.
A Study on Microturning with Electrochemical Assistance of the Cutting Process
Authors: Grabowski Marcin , Skoczypiec Sebastian , Wyszynski Dominik
Published: July 2018

The paper investigated an electrochemically-assisted microturning process. Depending on the variant of electrochemical assistance, material can be removed with simultaneous electrochemical and mechanical action or electrochemical assistance can change the conditions of the cutting by changing the mechanical properties of the machined material. The experimental part includes discussion of the study methodology and a comparison of straight turning results in the case of machining 1.4301 stainless steel with and without electrochemical assistance. Based on this study, we can confirm that electrochemical assistance brings significant benefits in both variants, especially when the depth-of-cut is in the range of 1 µm.
Antiviral Compounds from Myxobacteria
Authors: Mulwa Lucky S. , Stadler Marc
Published: July 2018

Viral infections including human immunodeficiency virus (HIV), cytomegalovirus (CMV), hepatitis B virus (HBV), and hepatitis C virus (HCV) pose an ongoing threat to human health due to the lack of effective therapeutic agents. The re-emergence of old viral diseases such as the recent Ebola outbreaks in West Africa represents a global public health issue. Drug resistance and toxicity to target cells are the major challenges for the current antiviral agents. Therefore, there is a need for identifying agents with novel modes of action and improved efficacy. Viral-based illnesses are further aggravated by co-infections, such as an HIV patient co-infected with HBV or HCV. The drugs used to treat or manage HIV tend to increase the pathogenesis of HBV and HCV. Hence, novel antiviral drug candidates should ideally have broad-spectrum activity and no negative drug-drug interactions. Myxobacteria are in the focus of this review since they produce numerous structurally and functionally unique bioactive compounds, which have only recently been screened for antiviral effects. This research has already led to some interesting findings, including the discovery of several candidate compounds with broad-spectrum antiviral activity. The present review looks at myxobacteria-derived antiviral secondary metabolites.
Enhancement of Antibacterial Activity of Orange Oil in Pectin Thin Film by Microemulsion
Authors: Jantrawut Pensak , Boonsermsukcharoen Kasidech , Thipnan Kanyanut , Chaiwarit Tanpong , Hwang Kyu-Mok , Park Eun-Seok
Published: July 2018

The purpose of this study was to prepare orange oil microemulsion (ME) and to investigate the antimicrobial activity of film containing orange oil ME. First, surfactants and co-surfactants were screened on their efficiency to form ME using pseudo-ternary phase diagrams. The influences of surfactant and co-surfactant mass ratios were studied and optimized ME-loaded-films were prepared. Then, films containing orange oil ME were characterized by SEM and texture analyzer, and then evaluated for antimicrobial activity against Staphylococcus aureus and Propionibacterium acnes using an agar disc diffusion method. The results showed that Tween 80 as surfactant and propylene glycol as co-surfactant at a 1:1 ratio possessed the maximum ME area. Three ME formulations of ME 20, ME 25, and ME 30, which consisted of 20, 25, and 30% w/v of orange oil were prepared, respectively. All ME formulations showed particle sizes of about 60.26–80.00 nm, with broad a polydispersity index of 0.42. The orange oil ME films exhibited higher elastic values than the control. The diameters of inhibition zones for FME 20, FME 25, and FME 30 against P. acnes were 13.64, 15.18, and 16.10 mm, respectively. Only the FME 30 had an antimicrobial activity against S. aureus with 8.32 mm of inhibition zone. Contrarily, the control film had no antimicrobial activity against both bacteria. In conclusion, the present study found that the antibacterial activity of orange oil in pectin thin film could be enhanced by preparing orange oil as an ME before loading into pectin thin film.
Identification of Nanocellulose Retention Characteristics in Porous Media
Authors: Aadland Reidun , Dziuba Carter , Heggset Ellinor , Syverud Kristin , Torsæter Ole , Holt Torleif , Gates Ian , Bryant Steven
Published: July 2018

The application of nanotechnology to the petroleum industry has sparked recent interest in increasing oil recovery, while reducing environmental impact. Nanocellulose is an emerging nanoparticle that is derived from trees or waste stream from wood and fiber industries. Thus, it is taken from a renewable and sustainable source, and could therefore serve as a good alternative to current Enhanced Oil Recovery (EOR) technologies. However, before nanocellulose can be applied as an EOR technique, further understanding of its transport behavior and retention in porous media is required. The research documented in this paper examines retention mechanisms that occur during nanocellulose transport. In a series of experiments, nanocellulose particles dispersed in brine were injected into sandpacks and Berea sandstone cores. The resulting retention and permeability reduction were measured. The experimental parameters that were varied include sand grain size, nanocellulose type, salinity, and flow rate. Under low salinity conditions, the dominant retention mechanism was adsorption and when salinity was increased, the dominant retention mechanism shifted towards log-jamming. Retention and permeability reduction increased as grain size decreased, which results from increased straining of nanocellulose aggregates. In addition, each type of nanocellulose was found to have significantly different transport properties. Experiments with Berea sandstone cores indicate that some pore volume was inaccessible to the nanocellulose. As a general trend, the larger the size of aggregates in bulk solution, the greater the observed retention and permeability reduction. Salinity was found to be the most important parameter affecting transport. Increased salinity caused additional aggregation, which led to increased straining and filter cake formation. Higher flow rates were found to reduce retention and permeability reduction. Increased velocity was accompanied by an increase in shear, which is believed to promote breakdown of nanocellulose aggregates.