bluuebunny/crossref_metadata_2025 huggingface dataset:
Psychological Well-Being of School Counsellors Model
Authors: Bali-Mahomed Nur Jannah , Ku-Johari Ku Suhaila , Mahmud Mohd Izwan , Amat Salleh , Saadon Syazwani
Published: January 2022

The school counselor’s role is increasingly challenging with various demands of students’ problems and the issue of Coronavirus disease (COVID-19) pandemic also affect students’ psychosocial and mental well-being. Therefore, school counselors need to equip themselves with high psychological well-being as a self-care factor to deal with burnout, instability, and work stress. This study aims to develop Psychological Well-Being Model among school counsellors. A total of 330 secondary school counsellors from four districts in Selangor were selected as the respondents using the group random sampling method. Data were collected through translated questionnaire instruments, namely Self Compassion Scale, Counselling Self Estimate Inventory, The Assessing Emotions Scale, Spiritual Involvement and Beliefs Scale Revised, and Psychological Well Being-Ryff. Confirmation Factor Analysis (CFA) and Structural Equation Modeling (SEM) show that there is a significant positive relationship between self-compassion, counselling self-efficacy, emotional intelligence, spiritual intelligence, and the school counsellor’s psychological well-being. The findings also showed that self-compassion, counselling self-efficacy, emotional and spiritual intelligence affected 76.5% (R2 = 0.765) of variance in psychological well-being. This study is one of the earliest in presenting the school counsellor’s psychological well-being model who can contribute to Malaysian education. The implications of this study suggest that the elements of self-compassion, counselling self-efficacy, emotional and spiritual intelligence, and psychological well-being should be applied in the curriculum at the counsellor training level in university so that counsellors have adequate preparation in providing effective services in schools. The Ministry of Education Malaysia, on the other hand, needs to cultivate psychological well-being interventions regularly so that counsellors can always manage various students’ issues in schools as well as maintaining psychological well-being in terms of personnel and professionals.
Exploratory and Confirmatory Factor Analysis for Disposition Levels of Computational Thinking Instrument Among Secondary School Students
Authors: Sovey Saralah , Osman Kamisah , Mohd-Matore Mohd Effendi Ewan
Published: January 2022

Computational thinking (CT) is a method for solving complex problems, but also gives people an inventive inspiration to adapt to our smart and changing society. Globally it has been considered as vital abilities for solving genuine issues successfully and efficiently in the 21st century. Recent studies have revealed that the nurture of CT mainly centered on measuring the technical skill. There is a lack of conceptualization and instruments that cogitate on CT disposition and attitudes. This study attends to these limitations by developing an instrument to measure CT concerning dispositions and attitudes. The instruments' validity and reliability testing were performed with the participation from secondary school students in Malaysia. The internal consistency reliability, standardized residual variance, construct validity and composite reliability were examined. The result revealed that the instrument validity was confirmed after removing items. The reliability and validity of the instrument have been verified. The findings established that all constructs are useful for assessing the disposition of computer science students. The implications for psychometric assessment were evident in terms of giving empirical evidence to corroborate theory-based constructs and also validating items' quality to appropriately represent the measurement.
Implementing Synchronous Online Flipped Learning for Pre-service Teachers During COVID-19
Authors: Lee Yong-Jik , Davis Robert , Li Yue
Published: January 2022

Most research has examined flipped learning within the context of face-to-face (F2F) instruction. However, previous research has not effectively explored the possibility of how online synchronous flipped learning influences pre-service teachers (PSTs) in teacher education programs during Coronavirus disease (COVID-19). Recognizing the gap in the literature, this paper explored three aspects of online synchronous flipped learning by understanding 1) PSTs' learner engagement, 2) self-directed learning, and 3) learner satisfaction in a Korean university. The data was collected from Korean PST's interviews, reflection notes, and course evaluations. The thematic analysis was used to analyze qualitative data sources. The study findings showed that PSTs favored a synchronous online FL because it encouraged them to engage in various collaborative activities through Zoom breakout sessions. Also, pre-class materials from online FL can positively enhance the PSTs' self-directed learning process. Based on these findings, this study provides suggestions on how to effectively implement online synchronous flipped learning in teacher education programs.
Moderating the Neuropsychological Impact of Online Learning on Psychology Students
Authors: Voloshyna Valentyna , Stepanenko Inna , Zinchenko Anna , Andriiashyna Nataliia , Hohol Oksana
Published: January 2022

The purpose of the study was to identify what neuropsychological effect online learning had on psychology students and how it could be moderated. The study was descriptive and combined qualitative and quantitative methods to address the research questions. The study relied on three phases such as baseline study, experiment, and reporting. The experiment utilised neuropsychology tests adopted from the NeurOn platform. It was found that the Psychology students’ perceptions of e-learning and their emotional reaction to them were found not to be appreciative. The practices in breathing exercises, meditation, or yoga were proved to be able to moderate the impact of online learning on the experimental group students’ attentional capacities, memory processes, and cognition abilities. The above findings were supported by the results obtained for the neuropsychology tests and the experimental group students’ self-reflections yielded from the use of the MovisensXS App. The students conﬁrmed that breathing exercises, meditation, or yoga reduced study stress and burnout caused by e-learning and improved their academic performance. The focus group online discussion also showed that integration of breathing exercises, meditation, and yoga helped the experimental group students keep emotional balance, concentrate on their studies easier, remember more information, and meet deadlines in completing assignments. The education scientists are suggested to study how the e-learning curriculum could be reshaped so that it used relaxation practices on regular basis.
Exploring the Role of Digital and Socio-civic Skills for Promoting Youth Participation and Digital Citizenship
Authors: Peart Mark , Cubo-Delgado Sixto , Gutiérrez-Esteban Prudencia
Published: January 2022

The emergence of digital technologies and a more global and digital society has brought about the need to develop and educate in Digital Citizenship, as well as to study how youth are taught to participate and learn citizenship in a digital age. This paper aims to explore the role of digital and socio-civic skills development, as facilitators for youth participation and analyses the relationship between sociodemographic variables (sex, age, educational level, and political ideology) with the participatory profile of participants. This is a study with a quantitative methodology, where, based on non-probabilistic convenience sampling, 534 young people between 16 and 35 years old from Spain, completed an online questionnaire regarding the development of digital and socio-civic skills. The results indicate how a participant’s participatory profile is related to other variables. In addition, significant differences are observed between the different participation profiles and digital and socio-civic skills, underlining that the development of digital and socio-civic skills are essential for educating in digital citizenship.
Effects of Generic and Subject-Didactic Teaching Characteristics on Student Performance in Mathematics in Secondary School: A Scoping Review
Authors: Spreitzer Carina , Hafner Samuel , Krainer Konrad , Vohns Andreas
Published: January 2022

Research on instructional quality has been of great interest for several decades, leading to an immense and diverse body of literature. However, due to different definitions and operationalisations, the picture of what characteristics are important for instructional quality is not entirely clear. Therefore, in this paper, a scoping review was performed to provide an overview of existing evidence of both generic and subject-didactic characteristics with regard to student performance. More precisely, this paper aims to (a) identify both generic and subject-didactic characteristics affecting student performance in mathematics in secondary school, (b) cluster these characteristics into categories to show areas for quality teaching, and (c) analyse and assess the effects of these characteristics on student performance to rate the scientific evidence in the context of the articles considered. The results reveal that teaching characteristics, and not just the instruments for recording the quality of teaching as described in previous research, can be placed on a continuum ranging from generic to subject-didactic. Moreover, on account of the inconsistent definition of subject-didactic characteristics, the category of ‘subject-didactic specifics’ needs further development to establish it as a separate category in empirical research. Finally, this study represents a further step toward understanding the effects of teaching characteristics on student performance by providing an overview of teaching characteristics and their effects and evidence.
Educational Robotics and Attention to Diversity: A Case Study
Authors: Fernández-Batanero José María , Piñero-Virué Rocío , Rodríguez-González César Antonio , Reyes-Rebollo Miguel María
Published: January 2022

In this study we focus our research on the case analysis of an eleven-year-old boy and his close relationship with technology, specifically robotics. The methodology of the study is experimental in nature, with the aim of improving the subject's attention span through robotics, thereby favouring his educational process and, consequently, his overall development. To this end, the attitudes, and aptitudes that this technological tool has provided the subject with are evaluated over a period of four years. Three data collection instruments were selected: questionnaire, interview, and observation. Among the conclusions we highlight, on the one hand, that the older the age and the greater the interest in robotics, the greater the individual's attention span and greater psychomotor coordination, increasing the improvement in the educational process and in their daily life. On the other hand, robotics is an effective way of orienting knowledge towards the personal and educational sphere and can provide advantages in integral development.  
Using Explicit Instruction of the International Phonetic Alphabet System in English as a Foreign Language Adult Classes
Authors: Trinh Quoc Lap , Nguyen Thi Dieu Lien , Le Thanh Thao
Published: January 2022

The effects of international phonetic alphabet (IPA) instruction on English as a foreign language (EFL) adult learners’ pronunciation have been well-recognized. However, not many studies on the topic were conducted in the Vietnamese context. Therefore, the current study aims to investigate (1) the impact of IPA learning on Vietnamese EFL adult learners’ pronunciation and (2) adult learners’ perceptions of the effects of learning the IPA system on their pronunciation. The study was designed as an experimental study, following a mixed-methods approach, using the pre-and-post-tests, questionnaires, and interviews to collect quantitative and qualitative data. Thirty-eight adult learners took part in this investigation; they were divided into two groups, nineteen in the control and nineteen in the experimental group. The experimental study lasted ten weeks before the questionnaires and interviews were administered with the participants in the experimental group. The results demonstrated a significant improvement in adult learners’ pronunciation in the experimental group. The participants in the experimental group also highly perceived the positive effects of learning the IPA system on their pronunciation. Pedagogical implications and suggestions were presented at the end of the paper.
Traditional conservation and human-primate conflict in Ujungjaya Village Community, Ujung Kulon, Banten, Indonesia
Authors: PERMANA SIDIK , Partasasmita Ruhyat , ISKANDAR JOHAN , ROHMATULLAYALY ENENG NUNUZ , ISKANDAR BUDIAWATI S. , MALONE NICHOLAS
Published: January 2022

Abstract. Permana S, Partasasmita R, Iskandar J, Rohmatullayaly EN, Iskandar BS, Malone N. 2020. Traditional conservation and human-primate conflict in Ujungjaya Village Community, Ujung Kulon, Banten, Indonesia. Biodiversitas 21: 521-529. In the past, rural Sundanese people’s interactions with wild animals, including nonhuman primates (hereafter ‘primates’), is influenced by traditional ecological knowledge (TEK) with foundations in various myths and beliefs. Today, because of environmental changes, development of a market economy, cultural change, and the enhancement of agricultural technology, the beliefs and practices associated with TEK have eroded. We aim to describe the present perceptions of primates by the Sundanese people of Ujungjaya Village, Sumur Subdistrict, Ujung Kulon, Banten Province, and demonstrate how these myths and beliefs manifest in behavior towards primates. We use qualitative methods based on an ethnobiological approach to gain insight into people’s perceptions of their natural surroundings. Our results show that the people of Ujungjaya still maintain deep perceptions that are manifested in stories, songs, poems, spells, and invocations that prohibit the killing of primates. However, on their own, these manifestations are insufficient to protect primates from harm as the penetration of market economies and the fragmentation of habitats create the conditions for increased human-primate conflict. Indeed, the people of Ujungjaya sometimes hunt and capture primates for consumption, trading, and medicinal use. As such, laws and regulations designed to promote conservation are insufficient without an understanding of the cultural and socio-economic aspects of people’s lives.
The occurrence of Anisakis larvae on hairtail, Trichiurus lepturus caught from the Pangandaran Waters, West Java, Indonesia
Authors: AYUN NISA QUROTA , DEWI LUTFI SEPTIANA , MURWANTOKO MURWANTOKO , Setyobudi Eko
Published: January 2022

Abstract. Ayun NQ, Dewi LS, Murwantoko, Setyobudi E. 2021. The occurrence of Anisakis larvae on hairtail, Trichiurus lepturus caught from the Pangandaran Waters, West Java, Indonesia. Biodiversitas 22: 1378-1384. This study aimed to investigate the prevalence, mean intensity, and type of Anisakis larvae infecting hairtail (Trichiurus lepturus) caught at Pangandaran Waters, West Java, Indonesia. It was conducted using 869 samples sourced from local fishermen. The parameters measured were the total length and weight of each T. lepturus. Furthermore, parasitological examination was carried out to investigate the occurrence of Anisakis larvae in the fishes. The selected samples of Anisakis were identified through morphological and molecular analysis. The results showed that the larvae were found either free in the abdominal cavity of the fishes or coiled and attached to the serosal layer of the gastrointestinal tract, gonad, liver, and muscle. The results showed that the larvae were found either free in the abdominal cavity of the fishes or coiled and attached to the serosal layer of the gastrointestinal tract, gonad, liver, and muscle. It also showed that their prevalence in the T. lepturus was at a moderate level (45.5%), with the mean intensity at 4.4 larvae/fish. Moreover, the larvae were mostly in the abdominal cavity (89%), as only a few were found in another part of the fishes. Morphological identification showed that the type of Anisakis present was type I, as they were characterized by the presence of mucron and ventriculus without caecum. Molecular analysis was carried out by sequencing the ITS region, and it confirmed that the nematode was Anisakis typica, a non-zoonotic parasite. These parasites were not present in the edible portion/muscle of the fishes, and had a relatively moderate prevalence and low intensity. Therefore, their presence posed a relatively low risk to human health. However, due to consumption patterns and the increasing popularity of seafood, being served raw or undercooked, the incidence of anisakiasis may increase in the future. In the other hand, the presence of these larvae might be used as a biological indicator for several ecological studies.
Trend of mosquito (Diptera: Culicidae) monthly distribution in Sanandaj County of Iran
Authors: Salavati Bahadin , ZAHIRNIA AMIR HOSSEIN , Nasirian Hassan , AZARI-HAMIDIAN SHAHYAD
Published: January 2022

Abstract. Salavati B, Zahirnia AH, Nasirian H, Hamidian SA. 2021. Trend of mosquito (Diptera: Culicidae) monthly distribution in Sanandaj County of Iran. Biodiversitas 22: 4705-4715. Mosquitoes are the most significant arthropods of medical importance due to their burden of transmitting pathogens and parasites. The present study was designed to investigate the distribution of mosquitos (Diptera: Culicidae) in Sanandaj County in 2018. Sanandaj County was divided into four areas in rural and urban areas, including north, south, east, and west. Four rural and urban areas were randomly selected from four geographical directions. A sampling of larval and adult mosquitoes was performed using standard methods. The dipping technique was used to collect the mosquito larvae. A sampling of adult mosquitoes was performed during feeding on humans and animal baits and resting in the natural indoors, outdoors, and artificial areas. Light traps were also used to collect adult mosquitoes. The larval and adult specimens were identified using the morphology-based key. In total, nine mosquito species, including Anopheles maculipennis s.l., Anopheles superpictus s.l., Culiseta longiareolata, Culiseta subochrea, Culex hortensis, Culex mimeticus, Culex modestus, Culex pipiens, and Culex theileri were inhabited in Sanandaj County. The larval and adult mosquito species trend remains constant for at least 6 months of a year, from May to October. One-way ANOVA didn't reveal a significant difference between mosquito breeding places' habitats and water types, mosquito localities and life stages, monthly mosquito distribution, and adult mosquito resting places. The frequency trends of larval and adult mosquito species remain constant at least for 6 months of a year. It needs to be ready for timely mosquito control measures.
Phytochemical screening and antioxidant activity of wild mushrooms growing in tropical regions
Authors: HERAWATI ELISA , RAMADHAN RICO , ARIYANI FARIDA , MARJENAH MARJENAH , KUSUMA IRAWAN WIJAYA , SUWINARTI WIWIN , MARDJI DJUMALI , AMIRTA RUDIANTO , ARUNG ENOS TANGKE
Published: January 2022

Abstract. Herawati E, Ramadhan R, Ariyani F, Marjenah, Kusuma IW, Suwinarti W, Mardji D, Amirta R, Arung ET. 2021. Phytochemical screening and antioxidant activity of wild mushrooms growing in tropical regions. Biodiversitas 22: 4716-4721. This study aims to determine the presence of secondary metabolites which generally have bioactivity that is antioxidant with phytochemical tests and antioxidant tests in Auricularia auricula, Schyzophyllum commune, Microporus xanthopus, and Trametes versicolor fungi that grow in the wild. Phytochemical analysis was carried out by testing color changes and modifications, while antioxidant tests were carried out by using a UV-Vis spectrophotometer and a DPPH solution (1,1-diphenyl-2-picrylhydrazyl). This research found that edible wood fungus that grows, wild such as A. auricula, contains secondary metabolites, namely flavonoids, triterpenoids, saponins, and tannins, but does not contain steroids, alkaloids, carotenoids, or coumarin, while S. commune contains secondary metabolites, namely flavonoids, steroids, tannins, and coumarin, but does not contain triterpenoids, alkaloids, carotenoids, or saponins and non-edible wood fungus, such as M. xanthopus, contains secondary metabolites, namely, flavonoids, saponins, tannins, coumarin, and steroids, but does not contain triterpenoids, alkaloids, or carotenoids, while T. versicolor contains compounds, secondary metabolites, flavonoids, saponin, tannins, coumarin, and triterpenoids, but does not contain steroids, alkaloids, or carotenoids. The antioxidant test results are based on IC50 calculations; it is known that the IC50 value of A. auricula is 499.25 g/mL, while S. commune is 121.37 g/mL, M. xanthopus is 251.20 g/mL, and T. versicolor is 493.04 g/mL. Based on the research results, edible and inedible wood mushroom extracts contain several phytochemicals and show potential for antioxidant activity.
Nitrogen-fixation and phosphate-solubilization bacteria isolated from alluvial and latosol soil paddy field
Authors: IKHWANI IKHWANI , SARIBANON NONON , SETIA TATANG MITRA , YUNIARTI ERNY , Jumakir Jumakir
Published: January 2022

Abstract. Ikhwani, Saribanon N, Setia TM, Yuniarti E, Jumakir. 2021. Nitrogen-fixation and phosphate-solubilization bacteria isolated from alluvial and latosol soil paddy field. Biodiversitas 22: 4722-4730. This study aims to isolate, select and identify molecular characterization of bacteria from alluvial and latosol soil paddy field. This study has been conducted from February-June 2020 in the Soil Biology Laboratory, ICALRRD, Bogor. Alluvial and latosol soil samples were selected from Pusakanagara and Muara experimental gardens, Muara district. The result showed that bacteria growth in N-free media was higher from latosol soil as compared to alluvial soil. The higher nitrogenase activity isolates, could form ethylene gas concentration 1.1.E + 09 µmol/mL/hours, 1.7.E + 09 µmol/mL/hours and 1.3.E + 09 µmol/mL/hours from  alluvial 6, latosol 4, and latosol 9.  Three isolates have great PSB performances, i.e. isolates No. 4.2, 2.1 and 2 could dissolve 14.398 mg l-1, 12.648 mg l-1 and 12.145 mg l-1 of phosphate, from latosol soil. The highest N-fixing and solubilization capacity identified by molecular characterization i.e latosol 4, 4.2 and alluvial 7.1, alluvial-1.11. The phylogeny analyzed the presence of bacterial isolates in two distinct groups. Latosol 3.1 and latosol 4.2 isolates clustered in the same as the genus Lysinibacillus bacteria. The Alluvial 7.1 bacterial isolate showed similarities by Fictibacillus sp. and alluvial 1.2 isolate showed similarities by the genus Bacillus sp.
Resilience response to yellow leaf curl disease and identification of resistance gene analogs (RGA) in pepper (Capsicum annuum)
Authors: Ayu Dyan Kusumaning , Maharijaya Awang , Syukur Muhamad , Hidayat Sri Hendrastuti
Published: January 2022

Abstract. Ayu DK, Maharijaya A, Syukur M, Hidayat SH. 2021. Resilience response to yellow leaf curl disease and identification of resistance gene analogs (RGA) in pepper (Capsicum annuum). Biodiversitas 22: 4731-4739. Pepper yellow leaf curl disease (PYLCD) caused by infection of Begomovirus is a serious threat to pepper production worldwide. Identification of the resistance gene analogs (RGA) and resilience response of pepper against PYLCD is needed especially for selection resistance genotype. Evaluation of resistance response involving 28 pepper genotypes was carried out through transmission of Begomovirus using whitefly (Bemisia tabaci) as vector. The result showed that IPB C12 and F4-012328-6-3 were potential resistance genotypes. A total of 15 R gene analogs (CaRGA) containing NBS motif, namely CARGA1 to CARGA15, were identified by degenerated PCR amplification and database mining. The alignment of deduced amino acid sequence revealed conservation of subdomains Ploop (GKTT), kinase2 (LVVLDDV), RNBSB/kinase3 (IILTTR) and GLPL. BLASTp analysis indicated that 15 RGA showed high homology at deduced amino acid level with R gene identified such as whitefly resistance protein Mi-1.2, Pvr 9 gene for potyvirus, Begomovirus resistance protein, TRGA15 and RGA 13 for putative late blight resistance. Phylogenetic analysis exhibited that isolated sequences distinguished into CNL-NBS groups. These pepper RGA could be considered as candidate sequences of resistance genes.
Inventory of native and mother trees in Key Biodiversity Areas of Cebu Island, Philippines for species selection in local reforestation programs
Authors: Lillo Edgardo P. , MALAKI ARCHIEBALD B. , ALCAZAR STEVE MICHAEL T. , ROSALES RAAMAH , REDOBLADO BERNARDO R. , DIAZ JOHN LOU B. , PANTINOPLE ERWIN M. , BUOT INOCENCIO E. JR
Published: January 2022

Abstract. Lillo EP, Malaki AB, Alcazar SMT, Rosales R, Redoblado BR, Diaz JLB, Pantinople EM, Buot Jr. IE. 2021. Inventory of native and mother trees in Key Biodiversity Areas of Cebu Island, Philippines for species selection in local reforestation programs. Biodiversitas 22: 4740-4749. Forest restoration is the counterforce of deforestation and forest degradation. Yet, despite promoting natural recovery after forest harvest, afforestation and reforestation efforts, tropical forest ecosystems still experience a poor ratio of forest loss to forest gain. The study assessed the native tree species and potential mother trees in different Cebu Island Key Biodiversity Areas (KBAs) as well as their distribution and habitat preferences which can be used in local reforestation programs. A vegetation survey was conducted in three KBAs in Cebu Island, namely Nug-as forest, Mount Lantoy and Mount Capayas to inventory all native species. Assessment based on the phenotypic characteristics of adult trees was also conducted for indicating mother trees. This study in Cebu Island KBAs recorded a total of 292 species, categorized into 125 families and 203 genera, which can be classified into native trees (210), vines and lianas (18), shrub (37), and herbs (27). Out of the 292 species, 214 were recorded in Nug-as forest, 172 in Mount Lantoy, and 145 in Mount Capayas. Of the 210 native tree species, Nug-as forest had 145 species, Mount Lantoy 131 species, and Mount Capayas 109 species. A total of 241 mother trees were identified in the three KBAs, corresponding to 77 species in which Nug-as forest had 143 trees correspond to 52 species, Mount Lantoy had 68 trees correspond to 29 species, and Mount Capayas had 31 trees correspond to 6 species. Such native tree species are recommended for reforestation programs as planting materials that could reduce the risks of failure due to its high adaptability to the environment.
Rice cultivar selection in an agroforestry system through GGE-biplot and EBLUP
Authors: Alam Taufan , Suryanto Priyono , Supriyanta Supriyanta , Basunanda Panjisakti , Wulandari Rani Agustina , Kastono Dody , Widyawan Muhammad Habib , NURMANSYAH Nurmansyah , TARYONO Taryono
Published: January 2022

Abstract. Alam T, Suryanto P, Supriyanta, Basunanda P, Wulandari RA, Kastono D, Widyawan MH, Nurmansyah, Taryono. 2021. Rice cultivar selection in an agroforestry system through GGE-biplot and EBLUP. Biodiversitas 22: 4750-4757. Genotype-by-environment interaction (GEI) causes differences in the productivity of rice cultivars in agroforestry systems. For this reason, the stability of rice cultivars is an important aspect that should be considered before a cultivar is recommended to farmers. Superior genotypes and ideal environments are commonly identified using two statistical models, namely, genotype–genotype-by-environment biplot (GGE-biplot) and empirical best linear unbiased prediction (EBLUP). In this study, 15 rice cultivars were evaluated in terms of their productivity and stability in three soil types (Lithic Haplusterts, Ustic Epiaquerts, and Vertic Haplustalfs) in an agroforestry system with kayu putih (Melaleuca cajuputi) in 2019 and 2020 at the Menggoran Forest Resort, Playen Forest Section, Yogyakarta Forest Management District, Indonesia. The cultivars were treated as random effects to select and obtain the EBLUP of the best cultivars in each soil type. The EBLUP revealed that Situ Patenggang showed the highest yields of 4.887 and 5.456 tons ha?1 in Lithic Haplusterts and Vertic Haplustalfs, respectively. GM 28 exhibited the highest yield of 6.492 tons ha?1 in Ustic Epiaquerts. Ciherang, GM 2, GM 8, GM 11, GM 28, Inpari 6 Jete, Inpari 33, IR-64, and Way Apo Buru were classified as stable and fairly stable cultivars, whereas the other cultivars were unstable. Therefore, rice cultivars with high yields in specific soil types should be selected.
Morphological response and genetic variability of four species of chili pepper (Capsicum spp.) under infection of pepper yellow leaf curl virus
Authors: Sayekti Tri Wahono Dyah Ayu , SYUKUR MUHAMAD , HIDAYAT SRI HENDRASTUTI , MAHARIJAYA AWANG
Published: January 2022

Abstract. Sayekti TWDA, Syukur M, Hidayat SH, Maharijaya A. 2021. Morphological response and genetic variability of four species of chili pepper (Capsicum spp.) under infection of pepper yellow leaf curl virus. Biodiversitas 22: 4758-4765. Chili pepper has various types and species, but only five known species are commonly used and consumed. Most cultivated chili is susceptible to various plant diseases, one of which is Pepper yellow leaf curl disease (PYLCD) caused by Pepper yellow leaf curl virus (PYLCV) (Begomovirus, Geminiviridae). To control PYLCD, resistant variety assembly is required to prevent virus infection in cultivated plants. From this research, testing on four chili species is expected to provide information regarding the resistance and performance of chili peppers to conditions infected with PYLCV. This study was conducted at Dramaga Bogor, West Java, Indonesia in two experimental units: planting under virus-free conditions (as control) and virus-infected conditions. Each experimental unit was carried out using a single factor Randomized Complete Block Design (RCBD) with three replications. Twenty-nine genotypes of chili pepper were used consisted of four species, including C. annuum, C. frutescens, C. chinense, and C. baccatum. Of the 29 genotypes tested, thirteen genotypes in the resistant, nine genotypes in moderate resistant, two genotypes in moderate susceptible, three genotypes in the Susceptible, and two genotypes in the highly susceptible category. The heritability, genotypic coefficient of variance (GCOV) and phenotypic coefficient of variance (PCOV) value obtained from testing for all characters is high, ranging from 65.16-99.12%, 14.87-82.60%, and 15.77-84.45%, respectively. Most of the genotypes from C. chinense showed good resistance to PYLCV. In general, by considering the category of the resistance level and other characters such as productivity, ‘Jolokia’ (C. chinense), ‘Anies’ (C. annuum) and ‘Bonita’ (C. frutescens) can be ascertained as potential candidate sources of resistance to PYLCV.
Representative benthic habitat mapping on Lovina coral reefs in Northern Bali, Indonesia
Authors: Kartikasari Anggita , PRISTIANTO TODHI , HANINTYO RIZKI , AMPOU EGHBERT ELVAN , WIBAWA TEJA ARIEF , BORNEO BERTA BERLIAN
Published: January 2022

Abstract. Kartikasari A, Pristianto T, Hanintyo R, Ampou EE, Wibawa TA, Borneo BB. 2021. Representative benthic habitat mapping on Lovina coral reefs in Northern Bali, Indonesia. Biodiversitas 22: 4766-4774. Satellite optical imagery datasets integrated with in situ measurements are widely used to derive the spatial distribution of various benthic habitats in coral reef ecosystems. In this study, an approach to estimate spatial coverage of those habitats based on observation derived from Sentinel-2 optical imagery and a field survey, is presented. This study focused on the Lovina coral reef ecosystem of Northern Bali, Indonesia to support deployment of artificial reefs within the Indonesian Coral Reef Garden (ICRG) programme. Three specific locations were explored: Temukus, Tukad Mungga, and Baktiseraga waters. Spatial benthic habitat coverages of these three waters was estimated based on supervised classification techniques using 10m bands of Sentinel-2 imagery and the medium scale approach (MSA) transect method of in situ measurement.The study indicates that total coverage of benthic habitat is 61.34 ha, 25.17 ha, and 27.88 ha for Temukus, Tukad Mungga, and Baktiseraga waters, respectively. The dominant benthic habitat of those three waters consists of sand, seagrass, coral, rubble, reef slope and intertidal zone. The coral reef coverage is 29.48 ha (48%) for Temukus covered by genus Acropora, Isopora, Porites, Montipora, Pocillopora. The coverage for Tukad Mungga is 8.69 ha (35%) covered by genus Acropora, Montipora, Favia, Psammocora, Porites, and the coverage for Baktiseraga is 11.37 ha (41%) covered by genus Montipora sp, Goniastrea, Pavona, Platygyra, Pocillopora, Porites, Acropora, Leptoseris, Acropora, Pocillopora, Fungia. The results are expected to be suitable as supporting data in restoring coral reef ecosystems in the northern part of Bali, especially in Buleleng District.
Estimating residents' willingness to pay for wetland conservation using contingent valuation: the case of Van Long Ramsar Protected Area, Vietnam
Authors: TRUONG DINH DUC
Published: January 2022

Abstract. Truong DD. 2021. Estimating residents' willingness to pay for wetland conservation using contingent valuation: the case of Van Long Ramsar Protected Area, Vietnam. Biodiversitas 22: 4784-4793. Willingness to pay (WTP) for wetland conservation is an important basis for designing market-based wetland protection strategies and sustainable wetland management. The main objective of this study is to estimate villagers' WTP for wetland conservation in Van Long Wetland Protected Area, Vietnam, and analyze factors influencing WTP. A questionnaire survey based on the dichotomous contingent valuation method (CVM) was conducted at seven communes surrounding Van Long Ramsar Protected Area (VLPA). The results showed that the local people in the studied area appreciate the roles of the wetland in preserving landscape values, supporting livelihoods, and transmitting values ??to future generations. The value of biodiversity conservation and the value of water filtration and regulation are perceived to a lesser extent in terms of the importance of conservation. Of the 384 respondents, 96% are WTP for wetland conservation at different levels. With the parametric model, the average value of WTP ranges from 300,000 to VND 328,000 VND /family/year. In the non-parametric model, the average of WTP is from 338,000 to 359,000 VND/family/year. The probability of environmental response' that willing to pay for conservation is closely related to their awareness, family income and payment amount. The results also showed optimistic points that the local people are willing to contribute to improving wetland quality. In a general sense, the results of this study make good contributions to the literature related to WTP for wetland conservation in developing countries.
Degradation of phytic acid from tithonia (Tithonia diversifolia) leaves using Lactobacillus bulgaricus at different fermentation times
Authors: Pazla Roni , YANTI GUSRI , JAMARUN NOVIRMAN , ARIEF Arief , ELIHASRIDAS ELIHASRIDAS , SUCITRA LARAS SUKMA
Published: January 2022

Abstract. Pazla R, Yanti G, Jamarun N, Arief, Elihasridas, Sucitra LS. 2021. Degradation of phytic acid from tithonia (Tithonia diversifolia) leaves using Lactobacillus bulgaricus at different fermentation times. Biodiversitas 22: 4794-4798. The aim of this study is to reduce high level of phytic acid in tithonia (Tithonia diversifolia) leaves by fermentation technique using Lactobacillus bulgaricus. A completely randomized design consisting of four treatments (fermentation time) i.e. T2: 2 days, T3: 3 days, T4: 4 days and T5: 5 days and experiment was performed in four replicates. The parameters measured were pH, phytase enzyme activity, total bacterial colony, phytic acid content, and phytic acid degradation. The results showed that the fermentation time had a significant effect (P<0.05) on pH, phytase enzyme activity, total bacterial colony, phytic acid content, and phytic acid degradation. The conclusion of this study is that on fifth day (Treatment T5) fermented tithonia leaves produced the lowest levels of phytic acid (3.48 mg/ 100g) with the highest level of degradation (64.81%).
Vegetation community and species association of Castanopsis spp. at its habitat in the remnant forest of Cibodas Botanical Garden, Indonesia
Authors: Nurdiana Dian ridwan , Buot Inocencio E , Jr.
Published: January 2022

Abstract. Nurdiana DR, Buot IE Jr. 2021. Vegetation community and species association of Castanopsis spp. at its habitat in the remnant forest of Cibodas Botanical Garden, Indonesia. Biodiversitas 22: 4799-4807. Castanopsis is one of the genera of Fagaceae with a large distribution in Indonesia. The genus can be found easily in Java, Sumatera and Kalimantan. Several studies have found an association between Castanopsis and environmental factors dealing with the diversity, dispersal and propagation. So far, there has been no agreement on how Castanopsis interacts with other species within the same genus in nature, especially in the remnant forest under a fragmented state. The objectives of the study were to determine the vegetation associated with Castanopsis and whether each species of the genus Castanopsis co-occur within the remnant forest? A total of 60 plots have been studied in 3 different sites of the remnant forest. The nested sampling method was used to identify the trees, saplings and seedlings. Sixty-seven total species of trees, 140 species of saplings and 121 species of seedlings in Cibodas Botanical Garden remnant forest were recorded. The diversity index for tree, sapling and seedling were 3.5, 3.9 and 4.1, respectively, while evenness index for tree, sapling and seedling were 0.81, 0.8 and 0.85 respectively. The interspecific association of the genus Castanopsis was restricted to C. javanica x C. tungurrut. They are associated positively at sapling stage with Jaccard index of 0.2. However, at tree stage, there was no association out of the pair, C. javanica x C. tungurrut. Competition within genus Castanopsis occurred in the same habitat, though at a lesser degree which needs to be studied further. Dominance of many pioneer species (Oreocnide integrifolia (Gaudich.) Miq., Ostodes paniculata Blume, Cestrum aurantiacum Lindl. and others) at tree stage had been evident. This implies that the Cibodas remnant forest is still in the process of succession towards a more stable climax state.
Feed intake of selected vegetables and fruits by coconut lorikeet (Trichoglossus haematodus) in a colony system
Authors: Prijono Siti Nuramaliati , RACHMATIKA RINI , MAHARANI SINTA
Published: January 2022

Abstract. Prijono SN,Rachmatika R, Maharani S. 2021. Feed intake of selected vegetables and fruits by coconut lorikeet (Trichoglossus haematodus) in a colony system. Biodiversitas 22: 4818-4828. Coconut lorikeet (Trichoglossus haematodus (Linnaeus 1771)) is nectarivorous bird. An imbalanced diet is a common problem with lorikeet were kept in captivity. The research method was completely randomized design arranged in a factorial with two factors. The first factor consisted of five types of vegetables or five types of fruits while the second factor consisted of five height levels of feed cup placement (30 cm, 60 cm, 90 cm, 120 cm and, 150 cm from the roof). This  study aimed to get information on the feed intake of selected vegetables and fruits that have high attractiveness to meet nutrient requirements and balanced diet for coconut lorikeets kept in a colony aviary. The feed intake of vegetable soybean (26.68 g) was higher than yardlong bean (21.63 g), green cabbage (19.12 g), red cabbage (16.24 g), and broccoli (14.27 g), while the feed intake of plum (14.15 g) was higher than apple (12.31 g), pomegranate (10.88 g), papaya (10.55 g) and guava (9.82 g). Vegetable and fruit intake by six coconut lorikeets in a colony system were 20.80% of the daily diet, and the siwalan sugar solution was 79.20% of the daily diet. It was suggested that the coconut lorikeets should be fed more vegetables than fruits. A good quality, varied and balanced diet will make the coconut lorikeets healthy and it will support future conservation effort.
Antibiotic resistance of Aeromonas spp. isolated from diseased walking catfish (Clarias sp.)
Authors: MULIA DINI SISWANI , Isnansetyo Alim , PRATIWI RARASTOETI , ASMARA WIDYA
Published: January 2022

Abstract. Mulia DS, Isnansetyo A, Pratiwi R, Asmara W. 2021. Antibiotic resistance of Aeromonas spp. isolated from diseased walking catfish (Clarias sp.). Biodiversitas 22: 4839-4846. Aeromonas spp. is known to be pathogenic to freshwater fish, including catfish. Antibiotics are often used to overcome bacterial attacks. However, the indiscriminate use of antibiotics, especially at incorrect doses and frequencies, could result in the emergence of resistant bacteria. The presence of resistance genes is also suspected to trigger the resistance potential of Aeromonas sp. against antibiotics. The objective of this study is to characterize the resistance genes of Aeromonas spp. isolated from diseased walking catfish (Clarias sp.). Ten Aeromonas spp. were isolated from infected walking catfish cultivated in Java. The resistance genes assessed included tetA, strA-strB, and qnrA. The antibiotics tested consisted of ampicillin (AMP), erythromycin (ERY), oxytetracycline (OXT), kanamycin (KAN), enrofloxacin (ENRO), and chloramphenicol (CHL). Results showed that some Aeromonas spp. had at least one out of the three genes assessed (i.e., tetA and strA-strB) but none had the qnrA gene. Aeromonas spp. were sensitive to KAN, ENRO, and CHL but resistant to AMP, ERY, and OXT. Thus, KAN, ENRO, and CHL are effective antibiotics for treating fish infected with Aeromonas spp.
Biodiversity of epiphytic periphyton in the leaves of the seagrass bed of Talawaan Bajo Estuary, North Sulawesi, Indonesia
Authors: Ogi Nova L.I.M. , Herawati Endang Yuli , Risjani Yenny , Mahmudi Mohammad
Published: January 2022

Abstract. Ogi NLIM, Herawati EY, Risjani Y, Mahmudi M. 2021. Biodiversity of epiphytic periphyton in the leaves of the seagrass bed of Talawaan Bajo Estuary, North Sulawesi, Indonesia. Biodiversitas 22: 4857-4864. This study aimed to analyze the diversity of epiphytic periphyton on the leaves of the seagrass bed of Talawaan Bajo estuary North Sulawesi Indonesia. The study was performed in three sampling points by using the line transect method with 50x50 cm2. The sampling points were located in front of the residential area, the mangrove forest, and in budo Cape. Seagrass and periphyton communities were analyzed for species density, relative frequency, diversity, evenness, and dominance index. Water quality and heavy metal Hg were also measured. The results showed that Cymodocea rotundata was the dominant seagrass based on species density and frequency distribution. Periphyton composition on the leaf of C. rotundata consisted of Bacillariophyceae (16 genera), Cyanophyceae (3 genera), Chlorophyceae (9 genera), Dinophyceae (1 genus), and Rhodophyceae (1 genus). The water quality, such as phosphate, current, nitrate, dissolved oxygen, and Hg content in the water, contributed to changing the environmental condition of Talawaan Bajo waters. Therefore, the efforts to manage coastal resource conservation in the Talawaan Bajo estuary require more concern from the government and stakeholders of Talawaan Bajo.
Short Communication: Fish diversity and its relationship with environmental variables in Kamala River, Nepal
Authors: Ghimire Sumnima , Koju Narayan
Published: January 2022

Ghimire S, Koju NP. 2021. Short Communication: Fish diversity and its relationship with environmental variables in Kamala River, Nepal. Biodiversitas 22: 4865-4871. Kamala River originates from the lower part of the Mahabharat range and flows through inner to outer Terai, providing a broad range of ecosystem services; provisioning, regulating, supporting, and cultural. However, the information regarding the environmental factors and species diversity in the river is not available enough. Hence the study aimed to explore baseline information on the diversity and abundance of fish and their relation to environmental variables. The fish diversity was assessed in five sections of Kamala River and correlated with different environmental variables. The study was carried out during September- October 2019, marking the post-monsoon season of Nepal. Fish samples were collected using cast nets, and physiochemical parameters were analysed onsite and in the laboratory. Altogether 19 freshwater fish species belonging to 5 orders, 8 families, and 15 genera were recorded. Cyprinids were the most dominant in the river, while Channids, Mastacembelids, Botiids, Sisorids, Gobiids were represented less. The Redundancy Analysis (RDA) ordination method revealed that species variation was correlated with temperature, Electrical Conductivity (EC), Total Dissolved Solids (TDS), and nitrate. Nonetheless, different river sections were disturbed due to mining, deforestation, and construction activities, which could pose a real threat to fish diversity and population, and other aquatic organisms.
Characteristics of anti-Vibrio harveyi compounds produced by Bacillus spp. isolated from shrimp ponds
Authors: Rusmana Iman , ISRAMILDA ISRAMILDA , Akhdiya Alina
Published: January 2022

Abstract. Rusmana I, Isramilda, Akhdiya A. 2021. Characteristics of anti-Vibrio harveyi compounds produced by Bacillus spp. isolated from shrimp ponds. Biodiversitas 22: 4872-4879. Pathogenic Vibrio spp. such as Vibrio vulnificus, V. alginolyticus, V. fluvialis, V. anguillarum, and V. harveyi caused shrimp diseases. The application of bacterial probiotics can control the growth of pathogenic Vibrio in shrimp. Bacillus spp. can produce antimicrobial compounds that inhibit the growth of pathogenic Vibrio spp. Isolation of Bacillus from several pond water samples, pond sediment, and shrimp intestines was successfully collected 175 isolates of Bacillus spp. Three isolates, i.e., Ltw54, Lts36, dan Lts40 had a high inhibitory index and stability in inhibiting the growth of Vibrio harveyi. Verification assay showed that Bacillus sp. Lts40 had the highest antimicrobial activity. The competition assay showed that Bacillus sp Lts40 isolates inhibited the growth of V. harveyi up to 81,8%. The antimicrobial compound produced by Bacillus sp. Lts40 was stable at the pH range of 3-11 and remained stable after heating at 100ºC for 20 minutes. The purification results using the chromatographic filtration technique showed that the protein fraction with a molecular weight of 47,38 kDa effectively inhibited V. harveyi growth. Bacillus sp. Lts40 isolate has potential application as a probiotic agent in shrimp ponds to control the growth of V. harveyi that causes luminous vibriosis diseases and the antimicrobial substance is potentially to be developed and produced as an anti-V. harveyi product that can be applied in a shrimp hatchery.