bluuebunny/crossref_metadata_2025 huggingface dataset:
Fate of Residual Pesticides in Fruit and Vegetable Waste (FVW) Processing
Authors: Nguyen Tri Thanh , Rosello Carmen , Bélanger Richard , Ratti Cristina
Published: October 2020

Plants need to be protected against pests and diseases, so as to assure an adequate production, and therefore to contribute to food security. However, some of the used pesticides are harmful compounds, and thus the right balance between the need to increase food production with the need to ensure the safety of people, food and the environment must be struck. In particular, when dealing with fruit and vegetable wastes, their content in agrochemicals should be monitored, especially in peel and skins, and eventually minimized before or during further processing to separate or concentrate bioactive compounds from it. The general objective of this review is to investigate initial levels of pesticide residues and their potential reduction through further processing for some of the most contaminated fruit and vegetable wastes. Focus will be placed on extraction and drying processes being amid the main processing steps used in the recuperation of bioactive compounds from fruit and vegetable wastes.
Characterization and Processing of Table Olives: A Special Issue
Authors: Gandul-Rojas Beatriz , Gallardo-Guerrero Lourdes
Published: October 2020

Table olives are recognized as an essential component of the Mediterranean diet, having been explicitly included in the second level of its nutritional pyramid as an aperitif or culinary ingredient, with a recommended daily consumption of one to two portions (15–30 g). Producers demand innovative techniques improving the performance and industrial sustainability, as well as the development of new products that respond efficiently to increasingly demanding consumers. The purpose of this special issue was to publish high-quality papers with the aim to cover the state-of-the-art, recent progress and perspectives related to characterization and processing of table olives. Two reviews offer an overview about the processing and storage effects on the nutritional and sensory properties of table olives, as well as the main technologies used for olive fermentation, and the role of lactic acid bacteria and yeasts characterizing this niche during the fermentation. A total of 10 research papers cover a broad range of aspects such as characterization of their chemical composition, bioavailability, advances in the processing technology, chemical and microbiological changes, optimized use of starter cultures for the improvement of the different fermentative processes, and new strategies to reduce sodium and additives to stabilize the organoleptic properties and avoid defects.
Determination of Polyvinyl Acetate in Chewing Gum Using High-Performance Liquid Chromatography–Evaporative Light Scattering Detector and Pyrolyzer–Gas Chromatography–Mass Spectrometry
Authors: Sim Sol , Kim Young-Min , Park Yeong-Ju , Siddiqui Mohammed Xain , Gang Yejin , Lee Jihyun , Lee Chan , Suh Hee-Jae
Published: October 2020

Polyvinyl acetate (PVAc) is used in various adhesive, paint, and transparent tape applications. It is also used as a food additive in food manufacturing to make chewing gum and fruit and vegetable glazes; however, guidelines on the amount of food additives that is used have not yet been established. In this study, a method was developed for analysis of polyvinyl acetate (PVAc) using high-performance liquid chromatography with an evaporative light scattering detector (HPLC–ELSD) and pyrolyzer–gas chromatography–mass spectrometry (Py–GC–MS). The analytical methods were applied to commercially available chewing gum. In the HPLC–ELSD analysis, the linearity was acceptable (R2 > 0.999), and the limits of detection and quantification were 22.2 and 67.3 µg/mL, respectively. The accuracies of PVAc were 87–115% at spike levels of 200–1000 µg/mL for the intra- and inter-day tests. The contents of PVAc in the chewing gum samples were n.d. (not detected)—13.8 g/kg. The presence of PVAc in chewing gum was verified with Py–GC–MS analysis, finding the typical pyrolysates of PVAc, such as acetic acid, benzene, toluene, styrene, indane, naphthalene, and acenaphthene. The developed analytical methods can be applied for successful identification of PVAc in chewing gum.
Tumor Regression in Lymph Node Metastases of Esophageal Adenocarcinomas after Neoadjuvant Therapy
Authors: Osecky Marek , Kröll Dino , Feith Marcus , Reim Daniel , Dislich Bastian , Becker Karen , Langer Rupert
Published: October 2020

Tumor regression following neoadjuvant treatment can be observed in lymph node (LN) metastases similar to the primary tumor in esophageal adenocarcinomas (EAC). We evaluated the prognostic significance of tumor regression in LN metastases of locally advanced EAC of 239 patients treated with neoadjuvant radiochemotherapy (RCTX) or chemotherapy (CTX) followed by esophagectomy. We examined retrospectively the LN for histopathologic signs of regression, i.e., nodular fibrosis and acellular mucin. LN classification was performed according to two parameters: presence (−) or absence (+) of residual tumor and regression characteristics in the LN, resulting in four categories: LN−/REG−, LN−/REG+, LN+/REG+, LN+/REG−. In total, LN metastases with residual tumor were detectable in 117/239 (49%) cases. Regression in LN were observed in 85/239 cases (35.5%). The distribution of the LN/REG categories were as follows: 97 patients (40.6%) were LN−/REG−. A total of 25 patients (10.5%) were LN−/REG+. A total of 60 (25.1%) were LN+/REG+ and 57 (23.8%) LN+/REG−. The LN/Reg categorization had a significant prognostic value in univariate analysis (p < 0.001) and multivariate analysis (HR = 1.326; p = 0.002) with similar results for the subgroups of patients treated with RCTX or CTX. The prognosis of LN−/REG+ was worse than LN−/REG− but better than both LN+ categories, which was demonstrated in the Kaplan–Meier curves but did not reach statistical significance (p = 0.104 and p = 0.090, respectively). In contrast, there was no difference between LN+/REG+ and LN+/REG− (p = 0.802). In summary, regression in LN metastases of EAC can be observed in a significant number of patients after neoadjuvant therapy. Complete regression of former LN metastases in comparison to “true” negative LN seems to be of prognostic relevance but additional studies are needed to confirm this trend seen in our study.
Cardiac Dimensions and Function are Not Altered among Females with the Myalgic Encephalomyelitis/Chronic Fatigue Syndrome
Authors: Iversen Per Ole , Lueder Thomas Gero von , Kardel Kristin Reimers , Lien Katarina
Published: October 2020

Background: Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a debilitating condition associated with several negative health outcomes. A hallmark of ME/CFS is decreased exercise capacity and often profound exercise intolerance. The causes of ME/CSF and its related symptoms are unknown, but there are indications of a dysregulated metabolism with impaired glycolytic vs oxidative energy balance. In line with this, we recently demonstrated abnormal lactate accumulation among ME/CFS patients compared with healthy controls after exercise testing. Here we examined if cardiac dimensions and function were altered in ME/CFS, as this could lead to increased lactate production. Methods: We studied 16 female ME/CFS patients and 10 healthy controls with supine transthoracic echocardiography, and we assessed cardiac dimensions and function by conventional echocardiographic and Doppler analysis as well as novel tissue Doppler and strain variables. Results: A detailed analyses of key variables of cardiac dimensions and cardiac function revealed no significant differences between the two study groups. Conclusion: In this cohort of well-described ME/CFS patients, we found no significant differences in echocardiographic variables characterizing cardiac dimensions and function compared with healthy controls.
Health-Care Services as a Platform for Building Community Resilience among Minority Communities: An Israeli Pilot Study during the COVID-19 Outbreak
Authors: Cohen Odeya , Mahagna Alaa , Shamia Asmaa , Slobodin Ortal
Published: October 2020

Background: Previous studies of minority groups in times of emergency have tended to focus on risk reduction or on individual resilience, overlooking the community factors that could be bolstered to promote better health and safety outcomes. The current study aimed to examine the role of health-care services in the perceived community resilience of urban and suburban Arab communities in Israel during the COVID-19 outbreak. Method: The study included 196 adults age 17–76 years, who filled out on-line questionnaires in May 2020; 112 participants lived in an urban community and 84 lived in a suburban community. Community resilience was evaluated using the Conjoint Community Resiliency Assessment Measure (CCRAM), a validated five-factor multidimensional instrument. Results: Residents of the suburban community reported higher community resilience than residents of the urban community. This difference was related to increased preparedness levels and strength of place attachment in the suburban community. Residents of suburban communities were also more satisfied and confident in health-care services than those of urban communities. Regression analysis showed that the satisfaction with primary health-care services, and not community type, significantly predicted community resilience. Conclusions: Our results support the pivotal role of primary health care in building community resilience of minority communities in times of emergency and routine.
Do Spatial Boundaries Matter for Exploring the Impact of Community Green Spaces on Health?
Authors: Shin Jong Cheol , Kwan Mei-Po , Grigsby-Toussaint Diana S.
Published: October 2020

Green space exposure is thought to have a positive influence on physical activity behavior and overall health. However, the literature remains equivocal, and green space measurement methods remain complicated. Using data from the Illinois Behavioral Risk Factor Surveillance System, this study examines the influence of green space on health-related factors, such as exercise, physical health, and mental health. Moreover, we explore the methods for measuring community green space via various spatial boundaries and green space resources. The results show that combining two contextually designated census boundaries and a measure of green space with seasonality were the best spatial conceptualizations for capturing community green space. Moreover, the findings showed a positive influence of green space exposure on health outcomes. These findings highlight the importance of considering geographic contexts of daily human behaviors and green space seasonality in providing a better understanding of the influence of community activity space on environmental exposure measurement. Further, this work contributes to community planning for encouraging health-promoting behaviors.
Synthetic Biology on Acetogenic Bacteria for Highly Efficient Conversion of C1 Gases to Biochemicals
Authors: Jin Sangrak , Bae Jiyun , Song Yoseb , Pearcy Nicole , Shin Jongoh , Kang Seulgi , Minton Nigel P. , Soucaille Philippe , Cho Byung-Kwan
Published: October 2020

Synthesis gas, which is mainly produced from fossil fuels or biomass gasification, consists of C1 gases such as carbon monoxide, carbon dioxide, and methane as well as hydrogen. Acetogenic bacteria (acetogens) have emerged as an alternative solution to recycle C1 gases by converting them into value-added biochemicals using the Wood-Ljungdahl pathway. Despite the advantage of utilizing acetogens as biocatalysts, it is difficult to develop industrial-scale bioprocesses because of their slow growth rates and low productivities. To solve these problems, conventional approaches to metabolic engineering have been applied; however, there are several limitations owing to the lack of required genetic bioparts for regulating their metabolic pathways. Recently, synthetic biology based on genetic parts, modules, and circuit design has been actively exploited to overcome the limitations in acetogen engineering. This review covers synthetic biology applications to design and build industrial platform acetogens.
Impact of A Cargo-Less Liposomal Formulation on Dietary Obesity-Related Metabolic Disorders in Mice
Authors: Komalla Varsha , Sheikholeslami Behjat , Li Gerard , Bokshi Bishwajit , Chan Yik Lung , Ung Alison , Gregory Oliver Brian , Chen Hui , Haghi Mehra
Published: October 2020

Current therapeutic options for obesity often require pharmacological intervention with dietary restrictions. Obesity is associated with underlying inflammation due to increased tissue macrophage infiltration, and recent evidence shows that inflammation can drive obesity, creating a feed forward mechanism. Therefore, targeting obesity-induced macrophage infiltration may be an effective way of treating obesity. Here, we developed cargo-less liposomes (UTS-001) using 1,2-dioleoyl-sn-glycero-3-phosphocholine, DOPC (synthetic phosphatidylcholine) as a single-agent to manage weight gain and related glucose disorders due to high fat diet (HFD) consumption in mice. UTS-001 displayed potent immunomodulatory properties, including reducing resident macrophage number in both fat and liver, downregulating liver markers involved in gluconeogenesis, and increasing marker involved in thermogenesis. As a result, UTS-001 significantly enhanced systemic glucose tolerance in vivo and insulin-stimulated cellular glucose uptake in vitro, as well as reducing fat accumulation upon ad libitum HFD consumption in mice. UTS-001 targets tissue residence macrophages to suppress tissue inflammation during HFD-induced obesity, resulting in improved weight control and glucose metabolism. Thus, UTS-001 represents a promising therapeutic strategy for body weight management and glycaemic control.
Editorial: Special Issue on “Molecular Mechanisms Regulating Osteoclastogenesis”
Authors: Brunetti Giacomina , Mori Giorgio , Faienza Maria Felicia
Published: October 2020

Bone is an active tissue that remodels continuously throughout life [...]
Combinatorial Effect of Cold Atmosphere Plasma (CAP) and the Anticancer Drug Cisplatin on Oral Squamous Cell Cancer Therapy
Authors: Lee Chang-Min , Jeong Young-IL , Kook Min-Suk , Kim Byung-Hoon
Published: October 2020

Cold atmospheric plasma (CAP) has been extensively investigated in the local treatment of cancer due to its potential of reactive oxygen species (ROS) generation in biological systems. In this study, we examined the synergistic effect of combination of CAP and cisplatin-mediated chemotherapy of oral squamous cell carcinoma (OSCC) in vitro. SCC-15 OSCC cells and human gingival fibroblasts (HGF-1) cells were treated with cisplatin, and then, the cells were irradiated with CAP. Following this, viability and apoptosis behavior of the cells were investigated. The viability of SCC-15 cells was inhibited by cisplatin with a dose-dependent manner and CAP treatment time. HGF-1 cells also showed decreased viability by treatment with cisplatin and CAP. Combination of 1 μM cisplatin plus 3 min of CAP treatment or 3 μM cisplatin plus 1 min of CAP treatment showed a synergistic anticancer effect with appropriate cytotoxicity against normal cells. ROS generation and dead cell staining were also increased by the increase in CAP treatment time. Furthermore, tumor-suppressor proteins and apoptosis-related enzymes also increased according to the treatment time of CAP. We showed the synergistic effect of cisplatin and CAP treatment against SCC-15 cells with low cytotoxicity against normal cells.
Performance of Force-Field- and Machine Learning-Based Scoring Functions in Ranking MAO-B Protein–Inhibitor Complexes in Relevance to Developing Parkinson’s Therapeutics
Authors: Murugan Natarajan Arul , Muvva Charuvaka , Jeyarajpandian Chitra , Jeyakanthan Jeyaraman , Subramanian Venkatesan
Published: October 2020

Monoamine oxidase B (MAOB) is expressed in the mitochondrial membrane and has a key role in degrading various neurologically active amines such as benzylamine, phenethylamine and dopamine with the help of Flavin adenine dinucleotide (FAD) cofactor. The Parkinson’s disease associated symptoms can be treated using inhibitors of MAO-B as the dopamine degradation can be reduced. Currently, many inhibitors are available having micromolar to nanomolar binding affinities. However, still there is demand for compounds with superior binding affinity and binding specificity with favorable pharmacokinetic properties for treating Parkinson’s disease and computational screening methods can be majorly recruited for this. However, the accuracy of currently available force-field methods for ranking the inhibitors or lead drug-like compounds should be improved and novel methods for screening compounds need to be developed. We studied the performance of various force-field-based methods and data driven approaches in ranking about 3753 compounds having activity against the MAO-B target. The binding affinities computed using autodock and autodock-vina are shown to be non-reliable. The force-field-based MM-GBSA also under-performs. However, certain machine learning approaches, in particular KNN, are found to be superior, and we propose KNN as the most reliable approach for ranking the complexes to reasonable accuracy. Furthermore, all the employed machine learning approaches are also computationally less demanding.
Current Status of Circulating Tumor DNA Liquid Biopsy in Pancreatic Cancer
Authors: Grunvald Miles W. , Jacobson Richard A. , Kuzel Timothy M. , Pappas Sam G. , Masood Ashiq
Published: October 2020

Pancreatic cancer is a challenging disease with a low 5-year survival rate. There are areas for improvement in the tools used for screening, diagnosis, prognosis, treatment selection, and assessing treatment response. Liquid biopsy, particularly cell free DNA liquid biopsy, has shown promise as an adjunct to our standard care for pancreatic cancer patients, but has not yet been universally adopted into regular use by clinicians. In this publication, we aim to review cfDNA liquid biopsy in pancreatic cancer with an emphasis on current techniques, clinical utility, and areas of active investigation. We feel that researchers and clinicians alike should be familiar with this exciting modality as it gains increasing importance in the care of cancer patients.
Towards Reduction or Substitution of Cytotoxic DMSO in Biobanking of Functional Bioengineered Megakaryocytes
Authors: Pogozhykh Denys , Eicke Dorothee , Gryshkov Oleksandr , Wolkers Willem F. , Schulze Kai , Guzmán Carlos A. , Blasczyk Rainer , Figueiredo Constança
Published: October 2020

Donor platelet transfusion is currently the only efficient treatment of life-threatening thrombocytopenia, but it is highly challenged by immunological, quality, and contamination issues, as well as short shelf life of the donor material. Ex vivo produced megakaryocytes and platelets represent a promising alternative strategy to the conventional platelet transfusion. However, practical implementation of such strategy demands availability of reliable biobanking techniques, which would permit eliminating continuous cell culture maintenance, ensure time for quality testing, enable stock management and logistics, as well as availability in a ready-to-use manner. At the same time, protocols applying DMSO-based cryopreservation media were associated with increased risks of adverse long-term side effects after patient use. Here, we show the possibility to develop cryopreservation techniques for iPSC-derived megakaryocytes under defined xeno-free conditions with significant reduction or complete elimination of DMSO. Comprehensive phenotypic and functional in vitro characterization of megakaryocytes has been performed before and after cryopreservation. Megakaryocytes cryopreserved DMSO-free, or using low DMSO concentrations, showed the capability to produce platelets in vivo after transfusion in a mouse model. These findings propose biobanking approaches essential for development of megakaryocyte-based replacement and regenerative therapies.
Rab GTPases in Osteoclastic Bone Resorption and Autophagy
Authors: Roy Michèle , Roux Sophie
Published: October 2020

Small guanosine triphosphate hydrolases (GTPases) of the Rab family are involved in plasma membrane delivery, fusion events, and lysosomal and autophagic degradation pathways, thereby regulating signaling pathways and cell differentiation and function. Osteoclasts are bone-resorbing cells that maintain bone homeostasis. Polarized vesicular trafficking pathways result in the formation of the ruffled border, the osteoclast’s resorptive organelle, which also assists in transcytosis. Here, we reviewed the different roles of Rab GTPases in the endomembrane machinery of osteoclasts and in bone diseases caused by the dysfunction of these proteins, with a particular focus on autophagy and bone resorption. Understanding the molecular mechanisms underlying osteoclast-related bone disease development is critical for developing and improving therapies.
Alpha-B-Crystallin Effect on Mature Amyloid Fibrils: Different Degradation Mechanisms and Changes in Cytotoxicity
Authors: Stepanenko Olga V. , Sulatsky M. I. , Mikhailova E. V. , Stepanenko Olesya V. , Povarova O. I. , Kuznetsova I. M. , Turoverov K. K. , Sulatskaya A. I.
Published: October 2020

Given the ability of molecular chaperones and chaperone-like proteins to inhibit the formation of pathological amyloid fibrils, the chaperone-based therapy of amyloidosis has recently been proposed. However, since these diseases are often diagnosed at the stages when a large amount of amyloids is already accumulated in the patient’s body, in this work we pay attention to the undeservedly poorly studied problem of chaperone and chaperone-like proteins’ effect on mature amyloid fibrils. We showed that a heat shock protein alpha-B-crystallin, which is capable of inhibiting fibrillogenesis and is found in large quantities as a part of amyloid plaques, can induce degradation of mature amyloids by two different mechanisms. Under physiological conditions, alpha-B-crystallin induces fluffing and unweaving of amyloid fibrils, which leads to a partial decrease in their structural ordering without lowering their stability and can increase their cytotoxicity. We found a higher correlation between the rate and effectiveness of amyloids degradation with the size of fibrils clusters rather than with amino acid sequence of amyloidogenic protein. Some external effects (such as an increase in medium acidity) can lead to a change in the mechanism of fibrils degradation induced by alpha-B-crystallin: amyloid fibers are fragmented without changing their secondary structure and properties. According to recent data, fibrils cutting can lead to the generation of seeds for new bona fide amyloid fibrils and accelerate the accumulation of amyloids, as well as enhance the ability of fibrils to disrupt membranes and to reduce cell viability. Our results emphasize the need to test the chaperone effect not only on fibrillogenesis, but also on the mature amyloid fibrils, including stress conditions, in order to avoid undesirable disease progression during chaperone-based therapy.
Fatty Acid Synthase Is a Key Enabler for Endocrine Resistance in Heregulin-Overexpressing Luminal B-Like Breast Cancer
Authors: Menendez Javier A. , Mehmi Inderjit , Papadimitropoulou Adriana , Vander Steen Travis , Cuyàs Elisabet , Verdura Sara , Espinoza Ingrid , Vellon Luciano , Atlas Ella , Lupu Ruth
Published: October 2020

HER2 transactivation by the HER3 ligand heregulin (HRG) promotes an endocrine-resistant phenotype in the estrogen receptor-positive (ER+) luminal-B subtype of breast cancer. The underlying biological mechanisms that link them are, however, incompletely understood. Here, we evaluated the putative role of the lipogenic enzyme fatty acid synthase (FASN) as a major cause of HRG-driven endocrine resistance in ER+/HER2-negative breast cancer cells. MCF-7 cells engineered to stably overexpress HRG (MCF-7/HRG), an in vitro model of tamoxifen/fulvestrant-resistant luminal B-like breast cancer, showed a pronounced up-regulation of FASN gene/FASN protein expression. Autocrine HRG up-regulated FASN expression via HER2 transactivation and downstream activation of PI-3K/AKT and MAPK-ERK1/2 signaling pathways. The HRG-driven FASN-overexpressing phenotype was fully prevented in MCF-7 cells expressing a structural deletion mutant of HRG that is sequestered in a cellular compartment and lacks the ability to promote endocrine-resistance in an autocrine manner. Pharmacological inhibition of FASN activity blocked the estradiol-independent and tamoxifen/fulvestrant-refractory ability of MCF-7/HRG cells to anchorage-independently grow in soft-agar. In vivo treatment with a FASN inhibitor restored the anti-tumor activity of tamoxifen and fulvestrant against fast-growing, hormone-resistant MCF-7/HRG xenograft tumors in mice. Overall, these findings implicate FASN as a key enabler for endocrine resistance in HRG+/HER2- breast cancer and highlight the therapeutic potential of FASN inhibitors for the treatment of endocrine therapy-resistant luminal-B breast cancer.
Genomic Features of a Food-Derived Pseudomonas aeruginosa Strain PAEM and Biofilm-Associated Gene Expression under a Marine Bacterial α-Galactosidase
Authors: Balabanova Larissa , Shkryl Yuri , Slepchenko Lubov , Cheraneva Daria , Podvolotskaya Anna , Bakunina Irina , Nedashkovskaya Olga , Son Oksana , Tekutyeva Liudmila
Published: October 2020

The biofilm-producing strains of P. aeruginosa colonize various surfaces, including food products and industry equipment that can cause serious human and animal health problems. The biofilms enable microorganisms to evolve the resistance to antibiotics and disinfectants. Analysis of the P. aeruginosa strain (serotype O6, sequence type 2502), isolated from an environment of meat processing (PAEM) during a ready-to-cook product storage (−20 °C), showed both the mosaic similarity and differences between free-living and clinical strains by their coding DNA sequences. Therefore, a cold shock protein (CspA) has been suggested for consideration of the evolution probability of the cold-adapted P. aeruginosa strains. In addition, the study of the action of cold-active enzymes from marine bacteria against the food-derived pathogen could contribute to the methods for controlling P. aeruginosa biofilms. The genes responsible for bacterial biofilm regulation are predominantly controlled by quorum sensing, and they directly or indirectly participate in the synthesis of extracellular polysaccharides, which are the main element of the intercellular matrix. The levels of expression for 14 biofilm-associated genes of the food-derived P. aeruginosa strain PAEM in the presence of different concentrations of the glycoside hydrolase of family 36, α-galactosidase α-PsGal, from the marine bacterium Pseudoalteromonas sp. KMM 701 were determined. The real-time PCR data clustered these genes into five groups according to the pattern of positive or negative regulation of their expression in response to the action of α-galactosidase. The results revealed a dose-dependent mechanism of the enzymatic effect on the PAEM biofilm synthesis and dispersal genes.
Experiences of Dynamic Identification and Monitoring of Bridges in Serviceability Conditions and after Hazardous Events
Authors: Rainieri Carlo , Notarangelo Matilde A. , Fabbrocino Giovanni
Published: October 2020

Operational Modal Analysis (OMA) currently represents an appealing technique for the non-destructive assessment and health monitoring of civil structures and infrastructures. Many applications have appeared in the literature in the last decade, demonstrating how this technique can support the observation and understanding of the structural behavior of bridges at different stages of their lifecycle and the remote detection of structural damage. The present paper describes some explanatory applications of OMA and modal-based Structural Health Monitoring (SHM) referring to bridges that have been carried out by the authors over the years. Some aspects related to the evolution of OMA in the last decade are summarized by means of the presentation and discussion of a number of case studies; they cover the fields of the non-destructive assessment and monitoring of bridges in serviceability conditions as well after hazardous events and remark the potential and the opportunities of OMA in the modern management of road infrastructures.
Developing a Universal and Efficient Method for the Rapid Selection of Stable Fluorescent Protein-Tagged Pathogenic Vibrio Species
Authors: Thorstenson Candice A. , Ullrich Matthias S.
Published: October 2020

World-wide increases in Vibrio-associated diseases have been reported in aquaculture and humans in co-occurrence with increased sea surface temperatures. Twelve species of Vibrio are known to cause disease in humans, but three species dominate the number of human infections world-wide: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus. Fluorescent protein (FP)-labelled bacteria have been used to make great progress through in situ studies of bacterial behavior in mixed cultures or within host tissues. Currently, FP-labelling methods specific for Vibrio species are still limited by time-consuming counterselection measures that require the use of modified media and temperatures below the optimal growth temperature of many Vibrio species. Within this study, we used a previously reported R6K-based suicide delivery vector and two newly constructed transposon variants to develop a tailored protocol for FP-labelling V. cholerae, V. parahaemolyticus, and V. vulnificus environmental isolates within two days of counterselection against the donor Escherichiacoli. This herein presented protocol worked universally across all tested strains (30) with a conjugation efficiency of at least two transconjugants per 10,000 recipients.
Quercitrin Ameliorates Hyperlipidemia and Hepatic Steatosis in Ovariectomized Mice
Authors: Hur Haeng , Jeong Yeon-Hui , Lee Sang , Sung Mi
Published: October 2020

Nonalcoholic fatty liver disease (NAFLD) is associated with progressive metabolic diseases. Estrogen deficiency increases the NAFLD risk among postmenopausal women. Thus, effective agents to prevent and treat NAFLD in postmenopausal women are required. Quercitrin (Quer) is a natural glycosylated flavonoid with antimicrobial, anti-inflammatory, and hypolipidemic effects. This study investigated whether Quer improves dysregulated lipid metabolism and suppresses hepatic steatosis in ovariectomized (OVX) mice as an experimental model mimicking postmenopausal women. Mice were assigned to the following four groups: SHAM, OVX, OVX + β-estradiol (0.4 mg/kg diet), and OVX + Quer (500 mg/kg diet). Mice were administered a diet with or without Quer for three months. OVX mice displayed significantly higher body mass, epidermal fat, and liver weights than those of SHAM mice. However, these levels were reduced in Quer-treated mice. Quer treatment reduced the levels of serum lipid metabolites, including triglycerides, total cholesterol, and low-density lipoprotein cholesterol. Furthermore, Quer reduced liver lipid steatosis and inhibited the expression of proinflammatory cytokines, such as tumor necrosis factor-α, IL-6, and IL-1β. The results of the present study indicate that Quer improves dysregulated lipid metabolism and reduces hepatic steatosis and inflammation by compensating for estrogen deficiency, suggesting that Quer may potentially exert protective effects during hepatic steatosis in postmenopausal women.
Measurement of Force Required for Anterior Displacement of Intraocular Lenses and Its Defining Parameters
Authors: Mochiji Mihoko , Kaidzu Sachiko , Ishiba Yoshihisa , Matsuda Yuji , Tanito Masaki
Published: October 2020

Intraocular stability during or after cataract and glaucoma filtration surgeries and vitreous surgery with a gas/silicone oil tamponade might differ among intraocular lenses (IOLs). We used six different one-piece IOL models and measured the force that displaced the IOLs from the vitreous cavity to anterior chamber as a measure of stability against the pressure gradient between the anterior and posterior IOL surfaces. We measured IOL hardness, haptics junction area, and posterior IOL bulge to identify what determines the IOL displacement force. The KOWA YP2.2 IOL (1.231 mN) required significantly greater force than the HOYA XY1 (0.416 mN, p = 0.0004), HOYA 255 (0.409 mN, p = 0.0003), Alcon SN60WF (0.507 mN, p = 0.0010), and Nidek NS60YG (0.778 mN, p = 0.0186) IOLs; J&J ZCB00V IOL (1.029 mN) required greater force than the HOYA XY1 (p = 0.0032) and HOYA 255 (p = 0.0029) IOLs; the Nidek NS60YG IOL required greater force than the HOYA 255 (p = 0.0468) IOL. The haptics junction area was correlated positively with the IOL displacement force (r = 0.8536, p = 0.0306); the correlations of the other parameters were non-significant. After adjusting for any confounding effects, the haptics junction area was correlated significantly with the IOL displacement force (p = 0.0394); the IOL hardness (p = 0.0573) and posterior IOL bulge (p = 0.0938) were not. The forces that displace IOLs anteriorly differed among one-piece soft-acrylic IOLs, and the optics/haptics junction area was the major force determinant.
Metallic Antibacterial Surface Treatments of Dental and Orthopedic Materials
Authors: Bai Rushui , Peng Liying , Sun Qiannan , Zhang Yunfan , Zhang Lingyun , Wei Yan , Han Bing
Published: October 2020

The oral cavity harbors complex microbial communities, which leads to biomaterial-associated infections (BAI) during dental and orthopedic treatments. Conventional antibiotic treatments have met great challenges recently due to the increasing emergency of drug-resistant bacteria. To tackle this clinical issue, antibacterial surface treatments, containing surface modification and coatings, of dental and orthopedic materials have become an area of intensive interest now. Among various antibacterial agents used in surface treatments, metallic agents possess unique properties, mainly including broad-spectrum antibacterial properties, low potential to develop bacterial resistance, relative biocompatibility, and chemical stability. Therefore, this review mainly focuses on underlying antibacterial applications and the mechanisms of metallic agents in dentistry and orthopedics. An overview of the present review indicates that much work remains to be done to deepen the understanding of antibacterial mechanisms and potential side-effects of metallic agents.
The Effect of Laser Parameters on Cutting Metallic Materials
Authors: Son Seungik , Lee Dongkyoung
Published: October 2020

This experimental study investigated the effect of laser parameters on the machining of SS41 and SUS304. The metallic materials play an important role in engineering applications. They are widely used in high-tech industries such as aerospace, automotive, and architecture. Due to the development of technology and high-tech industrialization, the various processing technologies are being developed with the requirement of high precision. However, the conventional cutting process is difficult to meet high precision processing. Therefore, to achieve high precision processing of the SS41 and SUS304, laser manufacturing has been applied. The current study investigated the process quality of laser cutting for SS41 and SUS304, with the usage of a continuous wave CO2 laser cutting system. The experimental variables are set to the laser cutting speed, laser power, and different engineering materials. The results are significantly affected by the laser parameters. As the result, the process quality of the laser cutting has been observed by measuring the top and bottom kerf widths, as well as the size of the melting zone and Heat Affected Zone (HAZ) according to volume energy. In addition, the evaluation of the laser processing parameters is significantly important to achieve optimal cutting quality. Therefore, we observed the correlation between the laser parameters and cutting quality. These were evaluated by analysis of variance (ANOVA) and multiple regression analysis. The experimental results of kerf top, kerf bottom, melting width, and HAZ on the laser parameters are properly predicted by multiple regression. In addition, the effect of laser parameters on the materials is determinant by the percentage of contribution of ANOVA.
Quasi-Situ Characterization of Retained Austenite Orientation in Quenching and Partitioning Steel via Uniaxial Tensile Tests
Authors: Gao Pengfei , Liu Jie , Chen Weijian , Li Feng , Pang Jingyu , Zhao Zhengzhi
Published: October 2020

As a representative of the third generation of advanced high strength steel, the quenching and partitioning steel has excellent potential in automobile manufacturing. The characterization and analysis of the mechanical properties and microstructure of the quenching and partitioning steel during deformation is an effective way to explore the microstructure evolution and transformation-induced plasticity effects of complex phase steels. The relationship between the microstructure morphology and mechanical properties of a 1180 MPa-grade quenching and partitioning steel was investigated through interrupted uniaxial tensile tests plus quasi-situ electron backscatter diffraction measurements. A mixture of ferrite, martensite, and retained austenite was observed in the microstructure. It was found that the volume fraction of global retained austenite decreased linearly with the increase of displacement (0 mm–1.05 mm). The evolution of the retained austenite with typical crystal direction ranges with deformation was characterized. Results show that the orientation (111) and (311) account for the highest proportion of retained austenite grains in the undeformed sample and the mechanical stability of the (311) retained austenite grains is the best. Moreover, the retained austenite grains rotated significantly in the early stage of the specimen deformation process (around yielding), and the work hardening of the specimen was weak at this stage, simultaneously.
Frost Damage in Tight Sandstone: Experimental Evaluation and Interpretation of Damage Mechanisms
Authors: Ding Shun , Jia Hailiang , Zi Fan , Dong Yuanhong , Yao Yuan
Published: October 2020

Low-porosity tight rocks are widely used as building and engineering materials. The freeze–thaw cycle is a common weathering effect that damages building materials in cold climates. Tight rocks are generally supposed to be highly frost-resistant; thus, studies on frost damage in tight sandstone are rare. In this study, we investigated the deterioration in mechanical properties and changes in P-wave velocity with freeze–thaw cycles in a tight sandstone. We also studied changes to its pore structure using nuclear magnetic resonance (NMR) technology. The results demonstrate that, with increasing freeze–thaw cycles, (1) the mechanical strength (uniaxial compressive, tensile, shear strengths) exhibits a similar decreasing trend, while (2) the P-wave velocity and total pore volume do not obviously increase or decrease. (3) Nanopores account for >70% of the pores in tight sandstone but do not change greatly with freeze–thaw cycles; however, the micropore volume has a continuously increasing trend that corresponds to the decay in mechanical properties. We calculated the pressure-dependent freezing points in pores of different diameters, finding that water in nanopores (diameter <5.9 nm) remains unfrozen at –20 °C, and micropores >5.9 nm control the evolution of frost damage in tight sandstone. We suggest that pore ice grows from larger pores into smaller ones, generating excess pressure that causes frost damage in micropores and then nanopores, which is manifested in the decrease in mechanical properties.
Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide
Authors: Lee Yoon , Park Yeoung-Hyun , Lee Dong-Seol , Seo You-Mi , Lee Ji-Hyun , Park Joo-Hwang , Choung Han-Wool , Park So-Hyun , Shon Won , Park Joo-Cheol
Published: October 2020

We aim to examine the effects of a newly developed peptide derived from CPNE7 (Cpne7-DP) in tertiary dentin formation and peritubular space occlusion, and comprehensively evaluate its potential as a bioactive therapeutic agent. Human dental pulp cells (HDPCs) and a mouse pre-odontoblast cell line, MDPC-23, were chosen for in vitro studies to characterize lineage-specific cell responses after Cpne7-DP treatment. Whether Cpne7-DP reproduces the dentin regenerative potential of CPNE7 was tested using a beagle dog model by generating dentinal defects of various degrees in vivo. Peritubular space occlusion was further examined by scanning electron microscopy and microleakage test, while overall mineralization capacity of Cpne7-DP was tested ex vivo. CPNE7 promotes tubular dentin formation under both shallow and deep dentinal defects, and the functional peptide Cpne7-DP induces odontoblast-like differentiation in vitro, mineralization ex vivo, and tubular dentin formation in in vivo beagle dog dentin exposure and pulp exposure models. Moreover, Cpne7-DP leads to peritubular space occlusion and maintains stability under different conditions. We show that CPNE7 and its derivative functional peptide Cpne7-DP promotes dentin regeneration in dentinal defects of various degrees and that the regenerated hard tissue demonstrates the characteristics of true dentin. Limitations of the current dental materials including post-operative hypersensitivity make biological repair of dentin a field of growing interest. Here, we suggest that the dual functions of Cpne7-DP in tubular dentin formation and peritubular space occlusion are promising for the treatment of dentinal loss and sensitivity.
Phase Change Materials Application in Battery Thermal Management System: A Review
Authors: Liu Changcheng , Xu Dengji , Weng Jingwen , Zhou Shujia , Li Wenjuan , Wan Yongqing , Jiang Shuaijun , Zhou Dechuang , Wang Jian , Huang Que
Published: October 2020

The purpose of a battery thermal management system (BTMS) is to maintain the battery safety and efficient use as well as ensure the battery temperature is within the safe operating range. The traditional air-cooling-based BTMS not only needs extra power, but it could also not meet the demand of new lithium-ion battery (LIB) packs with high energy density, while liquid cooling BTMS requires complex devices to ensure the effect. Therefore, phase change materials (PCMs)-based BTMS is becoming the trend. By using PCMs to absorb heat, the temperature of a battery pack could be kept within the normal operating range for a long time without using any external power. PCMs could greatly improve the heat dissipation efficiency of BTMS by combining with fillers such as expanded graphite (EG) and metal foam for their high thermal conductivity or coordinating with fins. In addition, PCMs could also be applied in construction materials, solar thermal recovery, textiles and other fields. Herein, a comprehensive review of the PCMs applied in thermal storage devices, especially in BTMS, is provided. In this work, the literature concerning current issues have been reviewed and summarized, while the key challenges of PCM application have been pointed out. This review may bring new insights to the PCM application.
On Fixed Points of Iterations Between the Order of Appearance and the Euler Totient Function
Authors: Hubálovský Štěpán , Trojovská Eva
Published: October 2020

Let Fn be the nth Fibonacci number. The order of appearance z(n) of a natural number n is defined as the smallest positive integer k such that Fk≡0(modn). In this paper, we shall find all positive solutions of the Diophantine equation z(φ(n))=n, where φ is the Euler totient function.
Data Science in Economics: Comprehensive Review of Advanced Machine Learning and Deep Learning Methods
Authors: Nosratabadi Saeed , Mosavi Amirhosein , Duan Puhong , Ghamisi Pedram , Filip Ferdinand , Band Shahab , Reuter Uwe , Gama Joao , Gandomi Amir
Published: October 2020

This paper provides a comprehensive state-of-the-art investigation of the recent advances in data science in emerging economic applications. The analysis is performed on the novel data science methods in four individual classes of deep learning models, hybrid deep learning models, hybrid machine learning, and ensemble models. Application domains include a broad and diverse range of economics research from the stock market, marketing, and e-commerce to corporate banking and cryptocurrency. Prisma method, a systematic literature review methodology, is used to ensure the quality of the survey. The findings reveal that the trends follow the advancement of hybrid models, which outperform other learning algorithms. It is further expected that the trends will converge toward the evolution of sophisticated hybrid deep learning models.
Fixed Point Results of Expansive Mappings in Metric Spaces
Authors: Yeşilkaya Seher Sultan , Aydın Cafer
Published: October 2020

In this study, we introduce the concept of θ-expansive mapping in ordered metric spaces and prove a fixed point theorem for such mappings. We give some fixed point results for θ-expansive mapping in metric spaces and prove fixed point theorems for such mappings. These results extend the main results of many comparable results from the current literature. We also obtain a common fixed point theorem of two weakly compatible mappings in metric spaces. Finally, the examples are presented to support the new theorems and results proved.
Geometric Inequalities of Bi-Warped Product Submanifolds of Nearly Kenmotsu Manifolds and Their Applications
Authors: Ali Akram , Mofarreh Fatemah
Published: October 2020

The present paper aims to construct an inequality for bi-warped product submanifolds in a special class of almost metric manifolds, namely nearly Kenmotsu manifolds. As geometric applications, some exceptional cases that generalized several other inequalities are discussed. We also deliberate some applications in the context of mathematical physics and derive a new relation between the Dirichlet energy and the second fundamental form. Finally, we present a constructive remark at the end of this paper which shows the motive of the study.
Coexistence of Periods in Parallel and Sequential Boolean Graph Dynamical Systems over Directed Graphs
Authors: Aledo Juan A. , Diaz Luis G. , Martinez Silvia , Valverde Jose C.
Published: October 2020

In this work, we solve the problem of the coexistence of periodic orbits in homogeneous Boolean graph dynamical systems that are induced by a maxterm or a minterm (Boolean) function, with a direct underlying dependency graph. Specifically, we show that periodic orbits of any period can coexist in both kinds of update schedules, parallel and sequential. This result contrasts with the properties of their counterparts over undirected graphs with the same evolution operators, where fixed points cannot coexist with periodic orbits of other different periods. These results complete the study of the periodic structure of homogeneous Boolean graph dynamical systems on maxterm and minterm functions.
Pet-Human Gut Microbiome Host Classifier Using Data from Different Studies
Authors: Bykova Nadia , Litovka Nikita , Popenko Anna , Musienko Sergey
Published: October 2020

(1) Background: microbiome host classification can be used to identify sources of contamination in environmental data. However, there is no ready-to-use host classifier. Here, we aimed to build a model that would be able to discriminate between pet and human microbiomes samples. The challenge of the study was to build a classifier using data solely from publicly available studies that normally contain sequencing data for only one type of host. (2) Results: we have developed a random forest model that distinguishes human microbiota from domestic pet microbiota (cats and dogs) with 97% accuracy. In order to prevent overfitting, samples from several (at least four) different projects were necessary. Feature importance analysis revealed that the model relied on several taxa known to be key components in domestic cat and dog microbiomes (such as Fusobacteriaceae and Peptostreptococcaeae), as well as on some taxa exclusively found in humans (as Akkermansiaceae). (3) Conclusion: we have shown that it is possible to make a reliable pet/human gut microbiome classifier on the basis of the data collected from different studies.