bluuebunny/crossref_metadata_2025 huggingface dataset:
Machine learning for high-resolution landslide susceptibility mapping: case study in Inje County, South Korea
Authors: Le Xuan-Hien , Eu Song , Choi Chanul , Nguyen Duc Hai , Yeon Minho , Lee Giha
Published: September 2023

Landslides are a major natural hazard that can significantly damage infrastructure and cause loss of life. In South Korea, the current landslide susceptibility mapping (LSM) approach is mainly based on statistical techniques (logistic regression (LR) analysis). According to previous studies, this method has achieved an accuracy of approximately 75.2%. In this paper, we expand upon this traditional approach by comparing the performance of six machine learning (ML) algorithms for LSM in Inje County, South Korea. The study employed a combination of geographical data gathered from 2005 to 2019 to train and evaluate six algorithms, including LR, Gaussian Naive Bayes (GNB), Support Vector Machine (SVM), K-Nearest Neighbors (KNN), Random Forest (RF), and Extreme Gradient Boosting (XGB). The effectiveness of these models was measured by various criteria, such as the percentage of correct classification (PCC) score, F1 score, and Kappa score. The results demonstrated that the PCC and F1 scores of the six models fell between [0.869–0.941] and [0.857–0.940], respectively. RF and XGB had the highest PCC and F1 scores of 0.939 and 0.941, respectively. This study indicates that ML can be a valuable technique for high-resolution LSM in South Korea instead of the current approach.
South African Life Sciences teachers’ pedagogical beliefs and their influence on information communication and technology integration
Authors: Ramnarain Umesh , Ncube Rumbidzai , Teo Timothy
Published: September 2023

Evidence suggests that learners actively construct knowledge and improve digital literacy when Information Communication and Technologies (ICTs) are used effectively. However, ICTs have not been exploited adequately in a science classroom. Early research argues that teachers’ pedagogical beliefs are potential barriers to delaying effective ICT integration in teaching and learning. In South Africa, research is silent regarding investigating Life Sciences teachers’ pedagogical beliefs translating into ICT integration. Hence, this study intended to close the knowledge gap and add to the body of knowledge. This study adopted a qualitative design to investigate three South African Life Sciences teachers pedagogical beliefs in Gauteng province. Qualitative data were collected from three Life Sciences teachers through lesson observations and semi-structured interviews. Teachers were observed teaching grade 11 Life Sciences classes. The video recordings were analyzed using the Teaching Dimension Observation Protocol (TDOP) to confirm findings from the questionnaire and establish how teachers integrate technology into teaching. Lastly, interviews were conducted to triangulate findings from lesson observations and questionnaires. The teachers integrated technology in ways that supported traditional beliefs. The results indicate the complexity of the relationship between pedagogical beliefs and ICT integration in practice. Teachers reflected on challenges in ICT integration that possibly impacted on their practices of ICT integration. They referred to a lack of learner access to smart devices and issues surrounding WiFi connectivity. In addition, teachers suggested that they needed training on innovative practices in ICT integration.
Nanoindentation response of small-volume piezoelectric structures and multi-layered composites: modeling the effect of surrounding materials
Authors: Cheng Guang , Wu Zonglin , Venkatesh T. A.
Published: September 2023

With piezoelectric small-volume composites gaining importance in smart device applications and nanoindentation being recognized as a versatile method for assessing the properties of layer materials, the present study is focused on the indentation response of the small-volume piezoelectric structures multi-layered composites. In particular, the effects of the nature of the substrate and surrounding materials, on the indentation response of piezoelectric nanocomposites, such as nanoislands, nanowires, and multi-layered composites are investigated. By developing three-dimensional finite element modeling, the complex interaction between the fundamental elastic, piezoelectric and dielectric properties of the piezoelectric materials and the elastic, plastic and electrically conducting or insulating properties of the surrounding materials, on the indentation response of the layered composites is analyzed. It is found that: (i) a substrate material that is elastically stiffer enhances the mechanical indentation stiffness and the electric indentation stiffness while plastic deformation in the substrate causes a reduction in the mechanical and electrical indentation stiffness; (ii) the effective piezoelectric and mechanical indentation stiffnesses of piezoelectric multi-layered composites are bounded by the corresponding characteristics of the bulk material counterparts from which the individual layers are constructed; (iii) electrically conducting surrounding materials produce a softening effect while insulating materials enhance the electrical indentation stiffness resulting in more charges being accumulated during the indentation process.
Factors affecting male-to-female ratio at birth in frozen-thawed embryo transfer cycles: a large retrospective cohort study
Authors: Du Tong , Xie Qin , Ye Jing , Wang Xindi , Qiu Jiaxin , Yan Zheng , Zhang Suqun , Zhao Dong , Lin Jiaying , Li Bin
Published: September 2023

Background

ICSI (intracytoplasmic sperm injection) leads to a reduced male-to-female ratio at birth, whereas blastocyst transfer results in an increased male-to-female ratio. However, limited knowledge exists regarding the impact of these factors on the live birth rate for each gender. This study aimed to investigate the influence of patient characteristics and treatment parameters on the live birth rate for each gender, as well as the ultimate male-to-female ratio at birth in frozen-thawed embryo transfer (FET) cycles.

Method

This retrospective cohort study involved a total of 28,376 FET cycles and 9,217 subsequent deliveries, spanning from January 2003 to December 2015. The study consisted of two parts. First, logistic regression models were constructed to determine the factors influencing the male-to-female ratio among babies born after FET. Second, we aimed to investigate the mechanisms underlying this sex ratio imbalance by analyzing data from all transfer cycles. Generalized estimated equations were employed to assess the impact of risk factors on rates of male and female live births separately.

Results

ICSI resulted in a lower proportion of male offspring compared to in vitro fertilization (IVF) (50.1% vs. 53.7%, aOR: 0.87, 95% CI: 0.80-0.96). Conversely, blastocyst transfer yielded a higher proportion of male offspring than cleavage-stage embryo transfer (58.7% vs. 51.6%, aOR: 1.32, 95% CI: 1.17-1.48). Analysis of all cycles indicated that ICSI resulted in a reduced likelihood of male live birth in comparison to IVF (19.8% vs. 21.6%, aOR: 0.90, 95% CI: 0.83-0.97). However, the transfer of blastocysts rather than cleavage-stage embryos not only increased the chance of male live birth (26.9% vs. 20.2%, aOR: 1.70, 95% CI:1.56-1.85) but also facilitated female live birth (20.3% vs. 19.3%, aOR: 1.26, 95% CI: 1.15-1.39).

Conclusion

ICSI was associated with a reduction in the male-to-female sex ratio and a lower rate of male live births, while blastocyst transfer was associated with an increased male-to-female sex ratio at birth and a higher rate of male live births.
Long-term glycemic variability predicts compromised development of heart failure with improved ejection fraction: a cohort study
Authors: Yang Chen Die , Chen Jia Wei , Quan Jin Wei , Shu Xin Yi , Feng Shuo , Aihemaiti Muladili , Ding Feng Hua , Shen Wei Feng , Lu Lin , Zhang Rui Yan , Wang Xiao Qun
Published: September 2023

Background

A substantial portion of heart failure (HF) patients adherent to guideline-directed medical therapies have experienced improved ejection fraction (EF), termed HFimpEF. Glycemic variability (GV) has emerged as a critical cardiometabolic factor. However, the relation between long-term GV and the incidence of HFimpEF is still unclear.

Methods

A total of 591 hospitalized HF patients with reduced EF (HFrEF, EF≤ 40%) admitted from January 2013 to December 2020 were consecutively enrolled. Repeat echocardiograms were performed at baseline and after around 12 months. The incidence of HFimpEF, defined as (1) an absolute EF improvement ≥10% and (2) a second EF > 40% and its association with long-term fasting plasma glucose (FPG) variability were analyzed.

Results

During a mean follow-up of 12.2 ± 0.6 months, 218 (42.0%) patients developed HFimpEF. Multivariate analysis showed FPG variability was independently associated with the incidence of HFimpEF after adjustment for baseline HbA1c, mean FPG during follow-up and other traditional risk factors (odds ratio [OR] for highest vs. lowest quartile of CV of FPG: 0.487 [95% CI 0.257~0.910]). Evaluation of GV by alternative measures yielded similar results. Subgroup analysis revealed that long-term GV was associated with HFimpEF irrespective of glycemic levels and diabetic conditions.

Conclusions

This study reveals that greater FPG variability is associated with compromised development of HFimpEF. A more stable control of glycemic levels might provide favorable effects on myocardial functional recovery in HF patients even without diabetes.
Intelligent optimization algorithm-based electricity pricing strategy for smart building clusters
Authors: Wang Hui , Liao Xu , Ji Xiu , Gong Shanggao , Meng Xiangping , Wang Jiarui
Published: September 2023

With the continuous infusion of renewable energy sources, smart buildings have evolved from single-load characteristics into dual characteristics with both electric energy production and consumption capability. Concurrently, the peak and off-peak periods of electricity consumption are influenced by climatic factors, which leads to complexity and deviation from the time-of-use tariffs set by electricity markets, which consequently result in a loss of revenue from grid-based electricity sales. Thus, adopting an innovative pricing mechanism to offset the revenue deficit in the grid assumes paramount significance. Built upon a dual-layer framework that employs intelligent optimization algorithms, this study proposes a pricing strategy for introducing the retail electricity provider into smart building clusters with peer-to-peer power sharing as the core. First, the independent operation model of intelligent buildings and electric energy sharing model without the participation of retail power suppliers are respectively established. Subsequently, with the aim to minimize alliance costs, a novel energy sharing pricing model involving retail electricity suppliers is developed, and a combination of particle swarm optimization and alternating direction multiplier methods is used for distributed solutions within a representative model. This approach yields optimal energy sharing transaction volumes and pricing while ensuring the confidentiality of each participating entity. Lastly, from the perspectives of the power grid, retail electricity suppliers, and multi-building smart alliances, this study conducts simulation analyses of key parameters that influence the bargaining effectiveness of retail electricity suppliers. These parameters encompass the upper limit of pricing, market supervision coefficient, and discount coefficient associated with the grid-based electricity sales to suppliers. Through these analyses, the study further validates the efficacy of the proposed strategy.
Does resource efficiency matter for environmental quality in Canada?
Authors: Kirikkaleli Dervis , Castanho Rui Alexandre , Deniz Özbay Rahmi , Yilmaz Genc Sema , Ahmed Zahoor
Published: September 2023

In order to combat climate change, the OECD emphasized the need to minimize the environmental impact of material use, as well as promote resource efficiency and accelerate the creation of a circular economy. The present study objects to promote a new debate about Canadian environmental quality and resource efficiency. In other words, this paper aims to capture the effect of resource efficiency on environmental quality in Canada while controlling financial development, economic growth, and energy. Nonlinear ARDL bounds test results indicate the significant long-run linkage between environmental quality, resource efficiency, financial development, economic growth, and energy in Canada. Moreover, the asymmetric results underline that 1) resource efficiency mitigates environmental degradation; 2) economic growth and energy uses in Canada significantly increase consumption-based CO2 emissions; 3) financial development positively contributes to environmental stability. Therefore, policymakers in Canada make sure that circular economies and resource efficiency can help reach net zero and combat climate change.
Time-dependent mortality and behavioral response of Odontotermes obesus (Blattodea: Termitidae) against different dose rates of pesticides for sustainable forest management
Authors: Aslam Asad , Chi De-Fu , Abbasi Asim , Arshad Muhammad , Hafeez Faisal , Fayyaz Amna , Hatamleh Ashraf Atef , Abdullah Al-Dosary Munirah
Published: September 2023

Termites have become a global concern, and their effective management has remained a challenge since time immemorial. Certain microbial and botanical agents have been used for their management, but their efficacy has been compromised, particularly in field conditions. Hence, the current study was designed to check the efficacy of low doses of different pesticides, such as chlorpyrifos, fipronil, bifenthrin, and chlorantraniliprole, against mortality and behavioral responses of Odontotermes obesus at two different temperatures (16 ± 1 and 26 ± 1°C). The discrete behavioral symptoms included intoxication, ataxia, moribundity, and death. Laboratory-maintained termite workers were exposed to different concentrations of pesticides through a filter paper bioassay. All tested pesticides and their concentrations differed significantly regarding their lethal time (LT50) values compared to the mortality of termite workers. Moreover, the LT50 values of pesticides gradually decreased with increased pesticidal concentrations. Temperature also had a significant effect on the efficacy of tested pesticides as all pesticides showed better results at higher temperatures. At both tested temperatures, chlorantraniliprole (5 ppm) proved to be the most effective pesticide against termite workers. Similarly, the behavioral symptoms also varied depending on pesticides and their administered concentrations and existed for a relatively longer time span at lower temperatures. In most cases, the order of responses was moribundity, followed by intoxication and ataxia. Moribundity and intoxication were the most frequently observed symptoms for chlorpyriphos and bifenthrin-treated termite workers. In the case of fipronil, intoxication was the most pronounced symptom. Similarly, the maximum value of ataxia was recorded in the case of chlorantraniliprole. However, moribund symptoms lasted longer in all tested concentrations of chlorantraniliprole, followed by ataxia and intoxication. The overall order of toxicity was chlorantraniliprole > bifenthrin > fipronil > chlorpyrifos. These pesticides, at their low doses, did not exhibit any repellent action and were not detected by the foraging termite workers. Moreover, their slow action mechanism makes them a suitable candidate for infecting whole colonies away from treated surfaces. Therefore, these pesticides can be successfully incorporated into different integrated termite management programs to keep the plantation free from threatening underground pests.
IL-21/23 axis modulates inflammatory cytokines and RANKL expression in RA CD4+ T cells via p-Akt1 signaling
Authors: Bhattacharya Gargee , Sengupta Soumya , Jha Rohila , Shaw Shubham K. , Jogdand Gajendra M. , Barik Prakash K. , Padhan Prasanta , Parida Jyoti R. , Devadas Satish
Published: September 2023

Introduction

CD4+ T cells are critically involved in the pathogenesis of Rheumatoid Arthritis; an autoimmune disorder characterized by joint inflammation and bone degeneration. In this study, we focused on the critical role of cytokines, IL-21 and IL-23 in facilitating the aberrant status of RA Th17-like cells and report their significant contribution(s) in modulating the expression of inflammatory cytokines and RANKL.

Methods

Blood and synovial fluid collected from a total of 167 RA patients and 25 healthy volunteers were assessed for various inflammatory markers and RANKL expression in plasma and CD4+ T cells. Subsequent ex vivo studies examined the role of specific cytokines, IL-21 and IL-23 in mediating inflammation and RANKL upregulation by blocking their expression with neutralizing antibodies in RA CD4+ T cells and terminally differentiated human Th17 cells. Further, the role of p-Akt1 as a signalling target downstream of IL-21 and IL-23 was evinced with IL-21 and IL-23 inhibition and phospho Akt-1/2 kinase inhibitor.

Results

Our observations highlighted the augmented inflammatory cytokine levels in plasma and an aberrant CD4+ T cell phenotype expressing exaggerated inflammatory cytokines and membrane RANKL expression in RA as opposed to healthy controls. Neutralization of either IL-21 or IL-23 (p19 and p40) or both, resulted in downregulation of the cytokines, TNF-α, IFN-γ and IL-17 and RANKL expression in these cells, signifying the critical role of IL-21/23 axis in modulating inflammation and RANKL. Subsequent dissection of the signaling pathway found p-Akt1 as the key phosphoprotein downstream of both IL-21 and IL-23, capable of increasing inflammatory cytokines and RANKL production.

Discussion

Our findings unequivocally identify IL-21/23 axis in RA CD4+ T cells as a key regulator dictating two critical processes i.e. exaggerated inflammation and higher RANKL expression and provide critical targets in their downstream signalling for therapeutic approaches.
Association between immunoglobulin G N-glycosylation and lupus nephritis in female patients with systemic lupus erythematosus: a case-control study
Authors: Lu Xinxia , Wang Liangao , Wang Meng , Li Yuejin , Zhao Qinqin , Shi Yanjun , Zhang Yujing , Wang Yingjie , Wang Wei , Ji Long , Hou Haifeng , Li Dong
Published: September 2023

Background

Lupus nephritis (LN) is a crucial complication of systemic lupus erythematosus (SLE) and has important clinical implications in guiding treatment. N-glycosylation of immunoglobulin G (IgG) plays a key role in the development of SLE by affecting the balance of anti-inflammatory and proinflammatory responses. This study aimed to evaluate the performance of IgG N-glycosylation for diagnosing LN in a sample of female SLE patients.

Methods

This case-control study recruited 188 women with SLE, including 94 patients with LN and 94 age-matched patients without LN. The profiles of plasma IgG N-glycans were detected by hydrophilic interaction chromatography with ultra-performance liquid chromatography (HILIC-UPLC). A multivariate logistic regression model was used to explore the associations between IgG N-glycans and LN. A diagnostic model was developed using the significant glycans as well as demographic factors. The performance of IgG N-glycans in the diagnosis of LN was evaluated by receiver operating characteristic (ROC) curve analysis, and the area under the curve (AUC) and its 95% confidence interval (CI) were calculated.

Results

There were significant differences in 9 initial glycans (GP2, GP4, GP6, GP8, GP10, GP14, GP16, GP18 and GP23) between women with SLE with and without LN (P < 0.05). The levels of sialylated, galactosylated and fucosylated glycans were significantly lower in the LN patients than in the control group, while bisected N-acetylglucosamine (GlcNAc) glycans were increased in LN patients (P < 0.05). GP8, GP10, GP18, and anemia were included in our diagnostic model, which performed well in differentiating female SLE patients with LN from those without LN (AUC = 0.792, 95% CI: 0.727 to 0.858).

Conclusion

Our findings indicate that decreased sialylation, galactosylation, and core fucosylation and increased bisecting GlcNAc might play a role in the development of LN by upregulating the proinflammatory response of IgG. IgG N-glycans can serve as potential biomarkers to differentiate individuals with LN among SLE patients.
Activation-induced deaminase expression defines mature B cell lymphoma in the mouse
Authors: Gómez-Escolar Carmen , Marina-Zárate Ester , Ramiro Almudena R.
Published: September 2023

Germinal centers (GCs) are the sites of secondary antibody diversification and underlie the mechanism of action of many vaccination strategies. Activation-induced deaminase (AID) triggers secondary antibody diversification through the introduction of somatic changes in immunoglobulin genes which lead to the generation of antibodies of higher affinity and more specialized effector functions. However, AID can also target other genomic regions, giving rise to mutations and chromosome translocations with oncogenic potential. Many human lymphomas originate from mature B cells that have undergone the GC reaction, such as the diffuse large B cell lymphoma, the follicular lymphoma and Burkitt lymphoma, and carry chromosome translocations. Mature B cell lymphomagenesis has been modeled in the mouse by the genetic introduction of chromosome translocations. Here, we present an in-depth characterization of one such model, λ-MYC mice. We found that young pre-tumor stage mice had a prominent block in early B cell differentiation that resulted in the generation of very aggressive tumors lacking surface B cell receptor (BCR) expression, indicating that a large fraction of tumors in λ-MYC mice arise from B cell precursors rather than from mature B cells. Further, we assessed the contribution of AID to B cell lymphomagenesis in λ-MYC mice by using a genetic tracer of historical AID expression. Only a fraction of tumors contained cells of GC origin as defined by AID expression. AID-experienced tumors associated with longer survival and resembled mature B cell lymphomas. Thus, AID expression defines Burkitt lymphomagenesis in λ-MYC mice.
A SST-constructed Ocean Heat Content index in crossing ENSO spring persistence barrier
Authors: Meng Xing , Chen Hui , Zhang Li , Jin Yishuai , Wu Lixin
Published: September 2023

The El Niño-Southern Oscillation (ENSO) spring persistence barrier (SPB) describes the feature in which the predictive skills of ENSO decrease significantly in the boreal spring. This paper investigates an index constructed using sea surface temperature (SST), namely SSTH, which is based on tropical Pacific Ocean Heat Content (OHC) in crossing ENSO SPB. Inspired by the dynamical relationship between the tropical Pacific OHC and eastern Pacific SST anomalies, SSTH is constructed by SST anomalies (i.e., Niño3.4 index) to represent OHC. We show that this index leads ENSO SST anomalies by about 10 months, making it effective in crossing ENSO SPB. Particularly, among the 50 ENSO events from 1950 to 2022, 27 years were identified to be caused by SSTH signals. Compared with warm water volume (WWV) or the west of WWV (WWVw), this index is more stable and effective after the 21st century because the effective region of subsurface OHC changed dramatically afterward. However, SSTH avoids this problem as it is constructed by SST anomalies alone. Finally, as SST data is reliable before 1980, SSTH is utilized to study the interdecadal lead-lag relationship between subsurface OHC and ENSO SST.
Molecular characterization, spatiotemporal expression patterns of fatty acid elongase (elovl8) gene, and its transcription changes in response to different diet stimuli in yellow catfish (Pelteobagrus fulvidraco)
Authors: Zeng Wan-Hong , Wei Xiu-Ying , Qin Wei , Qin Chuan-Jie , Shi Qiong , Guo Sheng-Tao , Prathomya Panita , Zhang Shi-Yong , Fu Peng , Hu Wei , Yuan Han-Wen , Wen Zheng-Yong
Published: September 2023

Elongase of very long-chain fatty acid 8 (Elovl8) is a new member identified in the Elovl family that is involved in the synthesis of highly unsaturated fatty acids (HUFAs). However, the evolutionary and physiological roles of this enzyme are still largely unknown. In the present study, the elovl8 gene was identified and characterized from yellow catfish Pelteobagrus fulvidraco, and then its evolutionary and molecular characteristics as well as transcriptional changes in response to various nutritional status were determined. Results showed that the open reading frame (ORF) of elovl8 was 795 bp in length, encoding a protein of 264 amino acids. Multiple sequences alignment showed that the yellow catfish Elovl8 was highly conserved with other homologs in teleosts, sharing similar structural characteristics (including six conserved transmembrane α-helical domains, four conserved elongase motifs, and three highly conserved cysteine residues). Meanwhile, comparisons of genetic synteny confirmed that the elovl8 gene identified from the yellow catfish was the homolog of elovl8b in other teleosts, and thus, the elovl8a gene was lost in the genome of the yellow catfish. Gene structure analysis revealed that the elovl8b gene contained eight exons and seven introns, which was highly conserved in teleosts, implying the functional conservation among various fish species. Tissue distribution analysis detected by real-time quantitative PCR (RT-qPCR) showed that the elovl8 gene was extensively expressed in all detected tissues except eyes, with high expression levels in the intestine and liver. Temporal expression analysis revealed that the expression level of elovl8 was stably expressed in the early 12 h after fertilization, and then dramatically decreased at 24, 48, 72, and 96 h after fertilization, implying that elovl8 is required for HUFA biosynthesis in the early development stages. Functional experiments showed that the expression of the elovl8 gene was stimulated after feeding with egg yolk but was not obviously affected after feeding with halogenated worms, indicating that diets full of HUFAs can inhibit the expression of elovl8 in yellow catfish. Our findings will help us to better understand the evolutionary and functional characteristics of elovl8 in teleosts, and lay a solid basis for investigating the regulation mechanism of HUFA biosynthesis.
Qualitative and quantitative endothelium changes after cataract surgery: ultrasound phacoemulsification vs. nanolaser technique
Authors: Zbiba Walid , Kharrat Malek , Sayadi Sana , Kallel Zeineb , Marzouk Ghassen
Published: September 2023

Purpose

The aim of this study was to evaluate corneal endothelial cell density and morphology, central corneal thickness, and best visual acuity using ultrasound (US) phacoemulsification or nanosecond laser technique.

Setting

Department of ophthalmology, Nabeul, Tunisia.

Design

Prospective cohort study.

Methods

This study included eyes with nuclear cataracts with a density grade of 1, 2, 3, or 4 according to LOCS III, divided into two groups; group 1 had conventional US, and group 2 had nanosecond laser. The endothelial cell density (ECD), coefficient of variation (CoV) in cell size, percentage of hexagonal cells, central corneal thickness (CCT) and best visual acuity (VA) were evaluated during 24 months.

Results

Seventy-four eyes had uneventful surgery, 40 in group 1, 34 in group 2. Three procedures in group 2 required conversion to standard phacoemulsification. The mean ECD decreased from 2616.4 ± 194.6 cells/mm2 in group 1 preoperatively to 2088.4 ± 229.9 after 2 years. In group 2, it decreased from 2611.8 ± 186.5 cells/mm2 to 2276.4 ± 163.8 after 2 years. The change was statistically significant in both groups. The decline of the mean ECD in group 2 was significantly less important than in group 1 (p = <10−2). The mean percentage of hexagonal cells was 45.18 ± 4.9 preoperatively and 43.5% ± 6.6 after 2 years in group 1. In group 2, it remained almost stable with 45.6 ± 5.1 and 45.4% ± 6.6 preoperatively and after 2 years, respectively. Preoperatively, the mean CoV was 0.39 ± 0.037 in group 1 and 0.38 ± 0.04 in group 2. After 2 years, it was 0.38 ± 0.04 and 0.37 ± 0.038 in group 1 and group 2, respectively. The changes of the mean CoV and the mean percentage of hexagonal cells were significant in both groups, but the difference between the groups was significant only during the six first months postoperatively. In preoperative, the mean corneal central thickness was 509.7 ± 19.5 in group 1 and 510.3 ± 20.4 in group 2. In both groups, the mean corneal thickness increased on D1 postoperatively to 550.9 in group 1, and 528.2 in group 2. The mean corneal thickness decreased more rapidly after 1 week in group 2, to find the initial values. Visual acuity improved from 0.76 Log Mar ± 0.5 at enrolment to 0.45 Log Mar ± 0.2, and 0.033 Log Mar ± 0.086 in group 1 at 1 day post-operative and after 24 months, respectively and from 0.58 Log Mar ± 0.28 to 0.2 Log Mar ± 0.09 and 0.035 Log Mar ± 0.083, respectively in group 2. There was no significant difference in VA at each follow-up between groups except for day 1.

Conclusion

Our study showed lower corneal tissue trauma, and lower endothelial cell loss in the laser cataract surgery compared to phacoemulsification.

Clinical trial registration: (https://classic.clinicaltrials.gov/ct2/show/NCT05886283), identifier NCT05886283.
Case report: lipoprotein apheresis reduces the risk of cardiovascular events and prolongs pregnancy in a woman with severely elevated lipoprotein(a), cardiovascular disease, and a high risk of preeclampsia
Authors: Marlȩga-Linert Joanna , Wartecka-Zielińska Katarzyna , Wydra Dariusz , Fijałkowski Marcin , Gruchała Marcin , Mickiewicz Agnieszka
Published: September 2023

Background

Preeclampsia is a common and serious pregnancy-induced disease, with potential severe maternal and fetal complications. Recently, an increased lipoprotein (a) (Lp[a]) concentration, an important factor in cardiovascular diseases (CVDs) pathogenesis, has been identified as a sensitive and specific marker of preeclampsia severity. Although lipoprotein apheresis (LA) is currently used in patients with hyperlipoproteinemia(a) and CVD, real-life data on its efficacy among pregnant women with an increased risk of preeclampsia are limited.

Case presentation

We present the case of a pregnant woman with severely elevated Lp(a), two previous episodes of the acute coronary syndrome and multivessel coronary disease treated with long-term LA before pregnancy, and a high risk of preeclampsia (as assessed using combined test screening). An increased pulsatility index and early diastolic notch were observed on Doppler interrogation at 18 weeks’ gestation. Biweekly LA therapy was re-initiated at 21 weeks’ gestation. The LA safely removed 70% of the serum Lp(a) concentration and reduced low-density lipoprotein-cholesterol (LDL-C) levels by 60%. We also observed an improvement in her urine protein/creatinine ratio, a reduction in the pulsatility index, and a notch on Doppler interrogation. The pregnancy lasted until week 36, when severe preeclampsia prompted an emergency cesarean delivery.

Conclusion

Pregnancy in women with elevated Lp(a), CVD, and a high risk of preeclampsia can present challenges in clinical management. Our case report indicates the benefits of LA in preventing atherosclerotic CVD progression during pregnancy, its potential influence on uteroplacental circulation, and prolongation of pregnancy for the best possible intrauterine fetus development. LA may be considered as a treatment option during pregnancy in such conditions. In addition, in pregnant women with CVD, we suggest screening using a combined test and measurement of Lp(a) as a marker of preeclampsia severity.
Prevalence and influencing factors of frailty in older patients with diabetes in China: a system review and meta-analysis
Authors: Liu Jian , Cao Yanjun , Wang Qingjie , Wang Zhiwei , Luan Xiaorong
Published: September 2023

Background and aims

To systematically evaluate the relevant literature to explore the prevalence and influencing factors of frailty in older patients with diabetes in China.

Methods

Cochrane Library, PubMed, Embase, Medline, CINAHL, Scopus, Proquest Central, Web of Science, SinoMed, CNKI, VIP and Wan fang Databases were searched to collect Chinese and English literatures about frailty in older diabetic patients. RevMan 5.4 software was used to extract data for systematic review.

Results

Seventeen studies involving 23,070 older patients with diabetes were included. The results showed that the prevalence of frailty in older Chinese diabetic patients was 30%. The main influencing factors were HbA1c level, number of complications, age, depression, exercise, and nutritional status.

Conclusion

The prevalence of frailty in Chinese elderly diabetic patients is high and there are many influencing factors. However, the quality of relevant literature is general and the number is limited, so high-quality prospective studies should be carried out in the future to further verify the conclusions.
Eucommia ulmoides Oliver repairs the disorder of intestinal microflora caused by high starch in Micropterus salmoides and improves resistance to pathogens
Authors: Liu Hongli , Li Fulong , Tang Hong , Chen Baipeng , Geng Yi , Chen Defang , Ouyang Ping , Li Liangyu , Huang Xiaoli
Published: September 2023

Eucommia ulmoides Oliver (EuO) is a natural medicine that can improve the composition of intestinal flora in fish, but more experiments and data are needed to support whether it can effectively improve the changes of intestinal flora and intestinal damage caused by high starch. This study examined the changes in intestinal structure as well as intestinal flora before and after the addition of EuO to high-starch diets and analyzed the effects of such changes on immune and digestive functions. The results showed that EuO reduces mortality during Nocardia seriolae attack and can reduce starch-induced intestinal inflammation. Eucommia ulmoides Oliver supplementation was able to alter the changes of intestinal flora in fatty acid degradation, bacterial chemotaxis, porphyrin metabolism and flagella assembly caused by high starch. By analyzing the abundance and correlation of bacterial communities, three bacterial communities that were significantly related to the intervention effect of EuO were screened. Further analysis revealed that EuO supplementation reduced the increase in abundance of Limnochordaceae, Nitrolancea, Lysinibacillus, and Hydrogenispora induced by high starch, which were negatively correlated with levels of the immunoreactive substance LZM in fish. This study reveals the regulatory effects of EuO on the intestinal flora of Micropterus salmoides fed on high starch diets, and provides a theoretical basis for reducing starch damage to fish in production.
The use of Xuanbai Chengqi decoction on monkeypox disease through the estrone-target AR interaction
Authors: Jiao Yanqi , Shi Chengcheng , Sun Yao
Published: September 2023

Introduction

After COVID-19, there was an outbreak of a new infectious disease caused by monkeypox virus. So far, no specific drug has been found to treat it. Xuanbai Chengqi decoction (XBCQD) has shown effects against a variety of viruses in China.

Methods

We searched for the active compounds and potential targets for XBCQD from multiple open databases and literature. Monkeypox related targets were searched out from the OMIM and GeneCards databases. After determining the assumed targets of XBCQD for monkeypox treatment, we built the PPI network and used R for GO enrichment and KEGG pathway analysis. The interactions between the active compounds and the hub targets were investigated by molecular docking and molecular dynamics (MD) simulations.

Results

In total, 5 active compounds and 10 hub targets of XBCQD were screened out. GO enrichment and KEGG analysis demonstrated that XBCQD plays a therapeutic role in monkeypox mainly by regulating signaling pathways related to viral infection and inflammatory response. The main active compound estrone binding to target AR was confirmed to be the best therapy choice for monkeypox.

Discussion

This study systematically explored the interactions between the bioactive compounds of XBCQD and the monkeypox-specific XBCQD targets using network pharmacological methods, bioinformatics analyses and molecular simulations, suggesting that XBCQD could have a beneficial therapeutic effect on monkeypox by reducing the inflammatory damage and viral replication via multiple pathways. The use of XBCQD on monkeypox disease was confirmed to be best worked through the estrone-target AR interaction. Our work could provide evidence and guidance for further research on the treatment of monkeypox disease.
Comparative genomic analysis reveals differential genomic characteristics and featured genes between rapid- and slow-growing non-tuberculous mycobacteria
Authors: Zhang Menglu , Wang Peihan , Li Cuidan , Segev Ofir , Wang Jie , Wang Xiaotong , Yue Liya , Jiang Xiaoyuan , Sheng Yongjie , Levy Asaf , Jiang Chunlai , Chen Fei
Published: September 2023

Introduction

Non-tuberculous mycobacteria (NTM) is a major category of environmental bacteria in nature that can be divided into rapidly growing mycobacteria (RGM) and slowly growing mycobacteria (SGM) based on their distinct growth rates. To explore differential molecular mechanisms between RGM and SGM is crucial to understand their survival state, environmental/host adaptation and pathogenicity. Comparative genomic analysis provides a powerful tool for deeply investigating differential molecular mechanisms between them. However, large-scale comparative genomic analysis between RGM and SGM is still uncovered.

Methods

In this study, we screened 335 high-quality, non-redundant NTM genome sequences covering 187 species from 3,478 online NTM genomes, and then performed a comprehensive comparative genomic analysis to identify differential genomic characteristics and featured genes/protein domains between RGM and SGM.

Results

Our findings reveal that RGM has a larger genome size, more genes, lower GC content, and more featured genes/protein domains in metabolism of some main substances (e.g. carbohydrates, amino acids, nucleotides, ions, and coenzymes), energy metabolism, signal transduction, replication, transcription, and translation processes, which are essential for its rapid growth requirements. On the other hand, SGM has a smaller genome size, fewer genes, higher GC content, and more featured genes/protein domains in lipid and secondary metabolite metabolisms and cellular defense mechanisms, which help enhance its genome stability and environmental adaptability. Additionally, orthogroup analysis revealed the important roles of bacterial division and bacteriophage associated genes in RGM and secretion system related genes for better environmental adaptation in SGM. Notably, PCoA analysis of the top 20 genes/protein domains showed precision classification between RGM and SGM, indicating the credibility of our screening/classification strategies.

Discussion

Overall, our findings shed light on differential underlying molecular mechanisms in survival state, adaptation and pathogenicity between RGM and SGM, show the potential for our comparative genomic pipeline to investigate differential genes/protein domains at whole genomic level across different bacterial species on a large scale, and provide an important reference and improved understanding of NTM.
Gene knockout of glutathione reductase results in increased sensitivity to heavy metals in Acidithiobacillus caldus
Authors: Shi Yuping , Wu Wei , Yang Yinghui , Liu Xiao , Lin Jianqiang , Liu Xiangmei , Lin Jianqun , Pang Xin
Published: September 2023

Acidithiobacillus caldus plays an important role in bioleaching of low-grade metal ore. It can promote the release of heavy metals in mining-associated habitats and survive in high concentrations of heavy metals. Functions of glutathione reductase (GR) in cell defense against reactive oxygen species caused by heavy metals have been elucidated in some eukaryotic cells and bacteria; however, no information is available in A. caldus. In this research, the methods of bioinformatics, gene expression, GR activity assays were used to detect and characterize the glutathione reductase gene from the A. caldus MTH-04 strain. Then, A. caldus gr knockout mutant and gr overexpression strain were constructed, and the heavy metal tolerant properties and transcriptional levels of ROS related genes of them were compared to study the function of GR. The results showed that, a putative gr gene F0726_RS04210 was detected in the genome of A. caldus MTH-04. The purified recombinant protein of F0726_RS04210 showed remarkable GR activity at optimal pH 7.0 and 30°C using in vitro assay. The evolutionary relationship of GR from A. caldus MTH-04 was close to that from Escherichia coli K12. Gene knockout or overexpression of gr in A. caldus did not affect the growth rate on S0 medium, suggesting that GR did not play a key role in the activation of sulfur. Deletion of gr resulted in increased sensitivity to heavy metals (Cu2+ and Zn2+) in A. caldus, and the gr overexpression strain showed enhanced tolerance to heavy metals. Furthermore, transcription analysis also revealed strong correlations between GR and the antioxidant pathway. The above results suggest that GR can play an important role in heavy metal tolerance in A. caldus.
Identification and characterization of a novel β-lactamase gene, blaAMZ–1, from Achromobacter mucicolens
Authors: Zhang Yuan , Zhao Jingxuan , Zhang Guozhi , Lin Naru , Sha Yuning , Lu Junwan , Zhu Tingting , Zhang Xueya , Li Qiaoling , Zhang Hailin , Lin Xi , Li Kewei , Bao Qiyu , Li Dong
Published: September 2023

Background

Achromobacter is a genus of gram-negative bacteria that can act as opportunistic pathogens. Recent studies have revealed that some species of Achromobacter show inherent resistance to β-lactams, but the resistance mechanisms of Achromobacter mucicolens have rarely been reported.

Method

The bacterium was isolated using standard laboratory procedures. The agar dilution method was used to determine the minimum inhibitory concentrations (MICs). Genome sequencing was performed using the PacBio RS II and Illumina HiSeq 2500 platforms, and the Comprehensive Antibiotic Resistance Database (CARD) was used to annotate the drug resistance genes. The localization of the novel β-lactamase AMZ-1 was determined, and its characteristics were determined via molecular cloning and enzyme kinetic analysis. The phylogenetic relationship and comparative genomic analysis of the resistance gene-related sequences were also analyzed.

Result

Achromobacter mucicolens Y3, isolated from a goose on a farm in Wenzhou, showed resistance to multiple antibiotics, including penicillins and cephalosporins. BlaAMZ–1 showed resistance to amoxicillin, penicillin G, ampicillin, cephalothin and cefoxitin, and the resistance activity could be inhibited by β-lactamase inhibitors. Enzyme kinetic analysis results showed that AMZ-1 has hydrolytic activity against a wide range of substrates, including cephalothin, amoxicillin, penicillin G, and cefoxitin but not ampicillin. The hydrolytic activity of AMZ-1 was greatly inhibited by avibactam but much more weakly inhibited by tazobactam. Mobile genetic elements could not be found around the blaAMZ–1-like genes, which are conserved on the chromosomes of bacteria of the genus Achromobacter.

Conclusion

In this study, a novel AmpC gene, blaAMZ–1, from the animal-origin bacterium A. mucicolens Y3 was identified and characterized. It conferred resistance to some penicillins and first- and second-generation cephalosporins. The identification of this novel resistance gene will be beneficial for the selection of effective antimicrobials to treat associated infections.
Hypoxia and lactate influence VOC production in A549 lung cancer cells
Authors: Furuhashi Takeshi , Matsumoto Yuki , Ishii Ryuga , Sugasawa Takehito , Ota Shigenori
Published: September 2023

Introduction: Cancer cells emit characteristic volatile organic compounds (VOCs), which are potentially generated from ROS-based lipid peroxidation of polyunsaturated fatty acids. The metabolism of such VOCs and their regulation remain to be fully investigated. In fact, the enzymes involved in the synthesis of these VOCs have not been described yet.

Methods: In this study, we firstly conducted in vitro enzyme assays and demonstrated that recombinant alcohol dehydrogenase (ADH) converted Trans 2-hexenal into Trans 2-hexenol. The latter has previously been reported as a cancer VOC. To study VOC metabolism, 14 different culture conditions were compared in view of Trans 2-hexenol production.

Results and discussion: The data indicate that hypoxia and the addition of lactate positively influenced Trans 2-hexenol production in A549 cancer cells. The RNAseq data suggested certain gene expressions in the VOC pathway and in lactate signaling, parallel to VOC production. This implies that hypoxia and lactate signaling with a VOC production can be characteristic for cancer in vitro.
Robust control for a tracked mobile robot based on a finite-time convergence zeroing neural network
Authors: Cao Yuxuan , Liu Boyun , Pu Jinyun
Published: September 2023

Introduction

Since tracked mobile robot is a typical non-linear system, it has been a challenge to achieve the trajectory tracking of tracked mobile robots. A zeroing neural network is employed to control a tracked mobile robot to track the desired trajectory.

Methods

A new fractional exponential activation function is designed in this study, and the implicit derivative dynamic model of the tracked mobile robot is presented, termed finite-time convergence zeroing neural network. The proposed model is analyzed based on the Lyapunov stability theory, and the upper bound of the convergence time is given. In addition, the robustness of the finite-time convergence zeroing neural network model is investigated under different error disturbances.

Results and discussion

Numerical experiments of tracking an eight-shaped trajectory are conducted successfully, validating the proposed model for the trajectory tracking problem of tracked mobile robots. Comparative results validate the effectiveness and superiority of the proposed model for the kinematical resolution of tracked mobile robots even in a disturbance environment.