bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-wide selection signal analysis of Australian Boer goat by using insertion/deletion variants
Authors: yuan ying , Yang Bai-Gao , he yongmeng , Duan xinghai , zhang weiyi , Na Ri-Su , Han yanguo , Zeng Yan , Huang Yongfu , Zhao Yong-Ju , Zhao Zhong-Quan , wang shizhi , e guangxin
Published: January 2024

Breed selection for commercial goat is needed for production efficiency,
growth trait alteration, and improved livestock quality. Boer goat is
famous for its stable production performance, fast growth rate, and high
meat production. Detecting selective signatures in its genome can
elucidate selection mechanisms for its economic and adaptive traits. In
this study, 1,122,858 InDels were identified based on the whole genomes
of 46 Australian Boer goats and 81 worldwide local goats. FST was used
to identify the candidate selection signatures in 127 goats. A total of
11229 InDels were obtained from the top1% of all inDels, and 1239
candidate genes were annotated. A total of 1193 and 476 candidate genes
were involved in 4726 GO terms and 299 KEGG pathways, respectively. Many
genes were related to muscle development (MEF2C, MAPK14, TMOD1 etc.),
reproduction (SRD5A1, FBXW11, DMRT1 etc.), immunity (CD200, SGK1, IL17RB
etc.) and metabolism (INSR, STXBP3, H6PD etc.). The results provide
novel and important insights into the genetic basis of selection in Boer
goat and may be useful for goat molecular breeding.
Media use in gynecological and obstetric care and women’s knowledge on lifestyle-related risks: A cross-sectional multi-center study
Authors: Wittek Maren , Wensing Michel , Ullrich Charlotte , Müller Gerhard , Heinzel-Gutenbrunner Monika , Bombana Manuela
Published: January 2024

OBJECTIVE: Media-based educational interventions applied by healthcare
providers may improve women‘s adherence, health literacy and awareness
of LRRFs, and off-spring health outcomes. This study aims to investigate
whether exposure to media-based educational interventions in
gynecological and obstetric care are associated with self-assessed
levels of knowledge on LRRFs during pregnancy and lactation. DESIGN: We
conducted a cross-sectional observational study. SETTING: The study was
carried out across 14 randomly generated sample points in the twelve
most-populated cities in Ba-den-Wuerttemberg, South-West Germany. Women
were recruited in gynecological and obstetric institutions. SAMPLE: The
study sample comprised of 219 women who met our inclusion criteria and
completed the quantitative questionnaire. METHODS: We applied ordinal
logistic regression analyses to calculate Odds Ratios (ORs) and 95%
Confidence Intervals (CIs). MAIN OUTCOME MEASURES: Women’s self-assessed
level of knowledge in relation to the exposure to media-based
educational interventions by healthcare providers. RESULTS: Media-based
educational interventions on LRRFs during pregnancy through
gynecologists and/or midwifes are significantly associated with women’s
self-assessed level of knowledge (gynecologists: OR 4.26 (95% CI 2.04,
8.90; p <.001); midwifes: OR 3.86 (95% CI 1.66, 8.98;
p=.002)). Similar results were found for media-based educational
interventions through gynecologists and/or midwifes on LRRFs during
lactation and its association with women’s self-assessed level of
knowledge (gynecologists: OR 4.76 (95% CI 2.15, 10.59; p
<.001); midwifes: OR 7.61 (95% CI 3.13, 18.53;
p<.001)). CONCLUSION: The study suggests that exposure to
media-based educational interventions in gynecological and obstetric
care increases women’s level of knowledge on LRRFs during pregnancy and
lactation.
Tympanoplasty with Boomerang Shaped Chondoperıchondrial Graft (Twbscg): Long-Term Results
Authors: Dundar Riza , İşlek Akif , yukkaldiran ahmet , Yağcı Tarık
Published: January 2024

Objectives: We aimed in this study to evaluate and present the long-term
results of type 1 tympanoplasty patients who were operated on by using
boomerang-shaped chondroperichondrial graft. Methods: The study was
designed retrospectively. One hundred and thirty-eight patients who
underwent primary type 1 tympanoplasty with tragal, boomerang-shaped
chondroperichondrial graft between September 2010 and March 2014 were
included. Patients invented to control examination between January 2021
and April 2021, and 62 patients complete control visits after
invitation. Audiological results and otomicroscopic findings were
recorded. The graft status, preoperative and postoperative air-bone gap
(ABG) values and presence of retraction were identified and compared
with preoperative findings. Results: The preoperative mean ABG value of
the patients was 23.69 ± 1.03 dB. Control ABG was calculated as 10.6 ±
0.7 dB (p<0.0001). The audiological test performed after a
mean of 68.5 + 14.2 (min: 60, max: 96) months after the surgery. The
average follow-up period was found as 73.03 ± 12.4 (min: 60, max: 122)
months. Perforation was detected in 6 (9.7 %) patients. Conclusion:
Considering the long-term results, we think that this grafting technique
of tympanoplasty has adequate and satisfactory results.
Conjunctival Impression Cytology and Dry Eye Parameters in Migraine Patients
Authors: Altaş Mustafa , Oltulu Pembe , Belviranlı Selman , Uca Ali Ulvi , Gündoğan Ali Osman , Mirza Enver , Doğru İsmet , Oltulu Refik
Published: January 2024

Objective In this study, it was aimed to compare ocular surface changes
and dry eye parameters between migraine patients and healthy
individuals. Material and Methods The participants of the present
cross-sectional comparative study are 30 migraine patients and 31 age
and gender matched healthy individuals. The demographic and clinical
data of migraine patients were recorded. Ocular surface changes were
evaluated using Schirmer I test, tear break-up time (TBUT), Ocular
Surface Disease Index (OSDI) scores and conjunctival impression cytology
(CIC) grades were assessed and results were compared between the two
groups. Results CIC degrees of the migraine patients were higher than
the control group (p<0.001). CIC assessment of the migraine
group revealed that ten patients (33.3%) were of grade II-III, whereas
the CIC assessment of two (6.5%) participants in the control group was
grade II. Grade III CIC was not determined in the control group. TBUT in
the migraine group was lower compared to the control group (p=0.023) and
Schirmer I was (p=0.013) lower and OSDI score higher
(p<0.001). In the migraine group, there was an inverse
correlation between CIC grade, Schirmer I test (p=0.001, ρ=-0.559) and
TBUT (p<0.001, ρ=-0.778). Conclusion Objective signs and
subjective symptoms of dry eye are common in patients with migraine.
Abnormal CIC evaluation findings such as morphological changes in the
bulbar conjunctiva, goblet cell loss, and squamous metaplasia suggest
the presence of inflammation. These findings too, support the hypothesis
that dry eye and changes in the ocular surface may play a role in the
pathogenesis of migraine.
Morphology-performance Mapping Determines Least Functional Resistance in Morphospace: A Case of Dual Locomotor Systems in Chinese Sturgeon
Authors: Qu Yi , Zhang Chaoshuo , Zhang Ying , Yang Gang , Hu Shaoqiu , Mi Xiangyuan , Zhang Dongxu , Cooke Steven , Rohlf Jim , Duan Ming
Published: January 2024

Morphology-performance (M-P) mapping seems to be predictive of how
morphology may evolve along “functional lines of least resistance”,
regardless of genetic effect, but as yet it is not clear the strict (or
mathematical) definition of the least functional resistance and how M-P
mapping determines it. We recorded station-holding and swimming
performance using a published critical swimming speed (Ucrit) test in
Chinese sturgeon Acipenser sinensis, and used Ucrit as a proxy for
fitness. We addressed a geometric morphometric framework to initiate
Arnold’s “morphology-performance-fitness” path model (Arnold, 1983),
assessing and visualizing apparent and direct M-P mapping. We quantified
them as that one unit of the most-performance-covaried shape vector
generated fapparent and f units of standardized performance,
respectively. We defined the least functional resistance as the
theoretically minimum morphological variation on an arbitrary direction
in morphospace required by one unit of standardized fitness,
(|b-1|min), which was calculated as a multivariate
function of direct M-P mapping (f), together with the angle between
paired most-performance-covaried shape vectors in morphospace (θ) and
direct performance effect on fitness (w), given dual performance
systems. The simulated and empirical data suggested that
|b-1|min was constrained by larger sum of absolute
direct effects (|fhold| + |fswim|)
and absolute functional interaction (|fhold fswim cos
θ|), and its direction was biased by magnitude of direct M-P
mapping.
Reliable estimation of butterfly flight period phenology from occurrence records
Authors: Larsen Elise , Shirey Vaughn
Published: January 2024

Previous phenological analyses of GBIF records ignored data biases and
produced misleading results. Here we demonstrate how data sufficiency
and methodology affect inferred latitudinal patterns in lepidopteran
phenology, highlighting the importance of spatiotemporal data
aggregation decisions. Results indicate later and/or shorter flight
period phenology at higher latitudes is common.
Quantitative Analysis of Tumor Cell Proliferation with Membrane Fluorescent Dye
Authors: Nie Biao , Yu Bingqing , Lin Chuangzhen , Zhong Hui , Zuo Junhua , Yang Ya-Feng , Zhang Zhaohui , Bai Wenke , Chen Lixin , Yang Yuping
Published: January 2024

Objectives: Cell membrane fluorescent dye known as DiO/DiD has been
suggested for its stability and safety as an effective tool for live
cell staining. This study developed a novel cell proliferation detection
technology based on DiO/DiD. Materials and Methods: Labelling of tumor
cells using membrane fluorescent dye DiO/DiD, and the fluorescence
intensity was measured by usig fluorescence-activated cell sorting. The
number of different tumor cell divisions was calculated through the
standard curves by measuring the fluorescence intensity. Labeled cells
was treated with different concentrations of doxorubicin to investigate
the effect of chemotherapy on labeled cells. DiO-labeled tumor cell were
injected into different organs and tissues to explore the relationship
between the number of cell divisions and tumor growth. Results: Tumor
cells can be divided into DiO-High and DiO-Low subgroups after
chemotherapy and a higher proportion of CD133 was observed in the
DiO-High cell subgroups compared to DiO-Low cell subgroups. Besides, the
tumor cells from metastatic tumors proliferated more rapidly than those
in situ. Conclusions: Application of cell membrane dye DiO/DiD in the
quantitative detection of cell proliferation method is expected to be
more feasible and applicable in future studies.
SUBLINGUAL METHYLCOBALAMIN TREATMENT IS AS EFFECTIVE AS INTRAMUSCULAR AND PERORAL TREATMENT IN 0-3 YEARS OLD CHILDREN
Authors: Kılıç Betül , KILIÇ Serhat , Eroğlu Elif Şahinoğlu , Gül Eylem , Apak Fatma Burcu Belen
Published: January 2024

Objectives: Vitamin B12 deficiency is a cause of preventable growth and
developmental retardation in children. In this respect, alternative
methods such as oral and sublingual treatments are being tried. We aimed
to compare the efficacy of oral, sublingual and intramuscular vitamin
B12 treatments in children aged 0-3 years. Methods: The study included
158 patients with serum vitamin B12 deficiency (serum vitamin B12 level
<300 pg/ml) aged 0-3 years retrospectively. According to the
vitamin B12 treatment modalities, the patients were divided into three
groups as oral cyanocobalamin (group 1), sublingual methyl-cobalamin
(group 2) and intramuscular cyanocobalamin (group 3). Results: The mean
values of vitamin B12 levels increased to above 300 pg/ml in all of
three groups . This increase was statistically significant for Group 1,2
and 3 (p<0.05). Conclusion: Sublingual methyl-cobalamin was
determined as effective as oral and intramuscular cyanocobalamin
improving vitamin B12 levels aged 0-3 years.
Assessment of Effects of Smoking Cessation on Lung Function and Quality of Life in Patients Admitted to the Smoking Cessation Outpatient Clinic
Authors: paksoy nur , Selçuk Engin Burak , Tetik Burcu Kayhan
Published: January 2024

Objective: Smoking is a significant health problem as the main cause of
many diseases. In this study we aimed to investigate the early stage
changes in the quality of life and the lung function following the
cessation of smoking and to investigate the relationship between these
changes in the patients who admitted to our smoking cessation outpatient
clinic. Methods: A total of 112 smoking cessation patients who admitted
to Turgut Ozal Medical Center Smoking Cessation outpatient clinic were
investigated. The patients answered a questionnaire, a health quality
test Quality of Life Assessment Scale (SF-36), Fagerström Nicotine
Addiction Test (FNAT) and they were given Pulmonary Function Test (PFT)
and the test and repeated at the end of the first and the third months.
“SPSS (Statistical Package for Social Sciences) for Windows 22.0” was
used for statistical analysis and a p< 0.05 was used to
determine statistical significance. Results: The analysis was carried
out on 112 individuals who applied to the smoking cessation polyclinic.
FEV₁(L), FVC (L) and FEF25-75 (L) values were significantly lower in
females compared to males and there was not any difference between the
groups in FEV₁/ FVC (L) values (p> 0.05). We observed no
improvement in terms of pulmonary function tests (PFT) after smoking
cessation whereas a significant development was observed in terms of
life quality (p< 0.05). Conclusion: We found that respiratory
functions did not improve after smoking cessation whereas the quality of
life improved significantly in the early period. While the effects of
smoking cessation on the respiratory functions and quality of life on
long term are emphasized in the literature, in this study we
demonstrated that the quality of life improves regardless of respiratory
functions in the early period of cessation.
Differences in complications between hepatitis B-related cirrhosis and alcohol-related cirrhosis
Authors: Zhuang Yu-Pei , Wang Si-Qi , Pan Zhao-Yu , Zhong Hao-Jie , He Xing-Xiang
Published: January 2024

Rationale, aims and objectives: Chronic hepatitis B virus (HBV)
infection and alcoholism are the leading cirrhotic etiologies. Cirrhosis
cases caused by different etiologies have different clinical features,
which leads to different clinical outcomes. This study aimed to
investigate the differences in complications between HBV- and
alcohol-related cirrhosis. Methods: Medical records of hospitalized
patients with HBV- or alcohol-related cirrhosis treated from January
2014 to January 2021 were retrospectively reviewed. The unadjusted rate
and adjusted risk of cirrhotic complications between the two groups were
assessed. Results: The rates of hepatocellular carcinoma (HCC) and
hypersplenism were notably higher in HBV-related cirrhosis (HCC: 39.55%
vs 1.45%, P < 0.001; hypersplenism: 45.84% vs 28.99%, P =
0.009), whereas the rates of hepatic encephalopathy (HE) and
acute-on-chronic liver failure (ACLF) were higher in alcohol-related
cirrhosis (HE: 15.94% vs 4.49%, P = 0.001; ALCF: 7.25% vs 2.28%, P =
0.040). After adjusting for potential confounders, HBV-related cirrhotic
patients still had higher risks of HCC (odds ratio [OR] = 34.06,
95% confidence interval [CI]: 4.61–251.77, P = 0.001) and
hypersplenism (OR = 2.29, 95% CI: 1.18–4.42, P = 0.014), while
alcohol-related cirrhotic patients still had higher risks of HE (OR=
0.22, 95% CI: 0.06–0.73, P = 0.013) and ACLF (OR = 0.30, 95% CI:
0.14–0.73, P = 0.020). Conclusion: HBV-related cirrhotic patients had
increased risks of HCC and hypersplenism, while alcohol-related
cirrhotic patients more readily developed HE and ACLF.
Well-posedness of solutions to a phase-field model for the martensitic phase transformations
Authors: yang manman
Published: January 2024

We study a phase-field model, which describes the transformations for
the austenite-martensite and the multiple twinning in Martensite. The
model consists of two nonlinear parabolic equations of second order. We
first show the existence of local solutions to an initial-boundary value
problem by utilizing the Banach fixed-point theorem. Then we verify the
solutions is global. Finally we investigate the regularity and
uniqueness of the solution.
Impact of COVID-19 pandemic on mental health during pregnancy and postpartum: A cross-sectional study
Authors: De Reena , Acharya Rudraprasad , Sasikumar Saranya , Sangma Pattianchi , Sil Amit , Mitra Sukumar , Mukhopadhyay Partha
Published: January 2024

Objective- To determine the prevalence of peripartum depression (PPD)
and anxiety during the COVID-19 pandemic, any differences between
COVID-infected and non-infected mothers and its risk factors. Study
design- Cross-sectional study. Setting- Tertiary care maternity hospital
Population- Pregnant and postpartum women Methods- Questionnaire based
direct interview. EPDS (Edinburgh postnatal depression scale) score,
COVID-19 anxiety scale (CAS) was used. Outcome measures- Possible PPD
(EPDS13), Probable PPD (EPDS14), Peripartum anxiety (EPDS anxiety sub
score4), COVID-19 related anxiety and risk factors of PPD. Results- Out
of 842 eligible subjects, 142 were confirmed COVID-19 positive and 670
patients were without the infection. The mean age was 25±3.9 years and
571 (67.8%) subjects were postpartum. Among the subjects, 383 (45.5%)
had possible depression, 317 (37.6%) had probable depression.
Peripartum anxiety was observed among 763(90.6%) subjects and the
median CAS score was 15(8-28). While there was no significant difference
in the prevalence of PPD, peripartum anxiety was higher among COVID
non-infected subjects (91.6% vs 86.6%, p=0.04). The CAS score was
higher among COVID-19 infected compared to the non-infected [17(10-28)
vs 15(8-25), p=0.00]. In multivariate analysis, history of psychiatric
illness [OR- 4.2(95% CI- 1.82-9.93), p=0.001], domestic violence
[OR-2.4(95% CI-1.16-5.10), p=0.019] and past obstetric
complications [OR-1.5(95%CI-1.01-2.27), p=0.042] were significant
risk factors for PPD. Conclusion- High prevalence of PPD was observed
during COVID-19 pandemic without any significant difference between
COVID-19 infected and non-infected subjects. COVID-19 infected patients
had higher COVID related anxiety. Funding- None taken Keywords-
peripartum depression; EPDS; COVID-19 anxiety scale.
“Prevalence of diabetic retinopathy in Eastern Mediterranean Region (EMR): A systematic review and meta-analysis”
Authors: Heiran Alireza , Azarchehry Seyede Pegah , Khalili Saeid Dehghan , Afarid Mehrded , Shaabani Sonia , Mirahmadizadeh Alireza
Published: January 2024

Objectives: Diabetic Mellitus is a global epidemic, exerting a
substantial burden on health care services. This high prevalence of
diabetes leads to an increase in diabetic complications, and diabetic
retinopathy is a leading cause of blindness and vision disability.
Individual studies on the prevalence of diabetic retinopathy in EMR
showed the high prevalence of diabetic retinopathy in EMR. Therefore, we
conducted a systematic review and meta-analysis of relevant studies to
estimate diabetic retinopathy in EMR. Method: We searched for the
relevant keywords in the title and abstract of Medline/PubMed, Scopus,
Embase, and Web of knowledge, as well as Google Scholar and manual
search to identify the published target studies from Jan 1st, 2000 to
December 13, 2019. The two-step screening was carried out by two
independent researchers and relevant data were extracted. Estimation of
summary proportions, subgroup analysis, meta-regressions, and
publication bias assessment was performed. Results: Amongst 4,139
identified citations, 109 articles were entered into a meta-analysis,
involving 280,566 patients. We found that the prevalence of diabetic
retinopathy was 30.67% (95% CI= 26.94%, 34.53%). Low HDI countries
and very high HDI countries had the highest 63.55% (95% CI= 52.37%,
74.04%) and the lowest 22.59 (95% CI= 20.53, 24.71%) diabetic
retinopathy prevalence. The meta-regression analysis showed that HDI had
a significant negative moderating effect (p <0.0001),
publication year had not a significant modulator effect (p = 0.6679),
and the mean duration of diagnosed diabetes had a significant positive
moderating effect (p <0.0001) on the prevalence of retinopathy
in diabetes. Conclusions: We estimated a high prevalence of diabetic
retinopathy in EMR. Our results implicate the importance of diabetes
screening, on-time and periodic examinations for retinopathy, diabetes
care, and risk factor controls.
MIXED CONVECTIVE FLOW OF HEAT AND MASS TRANSFER OF JEFFREY FLUID ON PERISTALTIC FLOW WITH NEWTONIAN FLUID
Authors: kalyan shreedevi , sharan ashwini
Published: January 2024

Mixed convective flow of transfer of heat and mass of Jeffrey fluid on
peristaltic flow alongwith Newtonian fluid in a vertical channel is
studied. Mathematical modelling, by using regular perturbation method
the coupled nonlineargoverning equations are solved analytically. The
effects of the various parameters and inclination angle on the velocity,
temperature and concentration are discussed through the graphical
representations and results are discussed. The consequences of Jeffrey
fluid parameters λ have been noted.
On Normal Congruence of Surfaces by Using Electromagnetic Wave Vectors: A Geometric Approach
Authors: Yavuz Ayşe
Published: January 2024

The present paper gives an extraordinary view of the normal congruence
of surfaces including the s lines and b lines in terms of
electromagnetic wave vectors in ordinary space. Frenet Serret frame of
given a space curve are described in E3 in terms of anholonomic
coordinates which includes eight parameters. Using the expression the
Frenet frames and electromagnetic wave vectors on the curve with a
linear transformation in terms of each other, the changes of !t , !E and
!B between any two points in the tangential and binormal direction along
with the curved path = (s; n; b) are obtained in terms of geometric
phase ; respectively. Intrinsic geometric properties of this normal
congruence of surfaces are obtained in terms of electromagnetic wave
vectors.
Prevalence of anemia among chronic myeloid leukemia patients treated with Imatinib: A evidence based meta-analysis.
Authors: Singh Avinash , Kumar Anoop , Agrawal Narendra , Bhurani Dinesh , Ahmed Rayaz , Sharma Manju
Published: January 2024

Aim- Imatinib is one of the tyrosine kinase inhibitors used for the
treatment of chronic myeloid leukemia (CML) patients. The exact
association of imatinib with anemia in CML patients is still unclear.
The current study aimed to find the prevalence of anemia in chronic
myeloid leukemia patients treated with imatinib. Methods- The relevant
articles were searched in PubMed, Google scholar, and Clinical trials
registries till 31st January 2021. The quality of the articles was
assessed using the Newcastle-Ottawa Scale. The prevalence rate with 95%
C. I was calculated using StatsDirect Statistical analysis software V.3.
Results A total of 18 studies containing 3,537 patients (male-1912 and
female-1478) were found relevant for the analysis. The pooled prevalence
of anemia in CML was found to be 34% (95% CI: 23%-46%). However, the
heterogeneity among studies was found to be high. Conclusion: The
physician should also take care of anemia while treating CML patients
with imatinib. Anemia is a major concern associated with CML patients
treated with Imatinib.
Microbial-derived carbon is a major contributor to rhizosphere soil organic carbon accumulation in alpine coniferous forests
Authors: Wang Qitong , Zhang Ziliang , Zhu Xiaomin , Liu Zhanfeng , Li Na , Xiao Juan , Liu Qing , Yin Huajun
Published: January 2024

Root activity creates a unique microbial hotspot in the rhizosphere and
profoundly regulates soil carbon (C) dynamics, but empirical assessments
of the soil microbial carbon pump (MCP) and associated ecological
consequences on soil C storage based on insight of the rhizosphere
remains lack, especially for different root functional modules. Based on
the assessment of MCP efficacy in the rhizosphere of absorptive and
transport roots, we found that rhizosphere MCP efficacy was
~ 60%. The RMCP-efficacy was 26.5% higher in the
rhizosphere of absorptive roots than in that of transport roots. The
plant-derived C contributed only 5.71 ~ 10.48% of the
increased SOC in the rhizosphere. These observations suggest that the
soil MCP is intensely stimulated in the rhizosphere, especially in the
rhizosphere of absorptive roots. Our study provides novel and direct
empirical evidence for the active soil MCP functions in SOC
sequestration from the perspective of the rhizosphere.
Global well-posedness for the Cauchy problems of the 3d simplified Ericksen-Leslie system with Fujita-Kato type initial data
Authors: Qiao Liu
Published: January 2024

In this paper, we provide the global well-posedness of the Cauchy
problems of the 3d simplified Ericksen-Leslie system, modelling the
incompressible nematic liquid crystal flows, with Fujita-Kato type
initial data. More precisely, we prove that for any initial data
$(u_0,d_0)\in
H^{s}(\mathbb{R}^3)\times
H^{s+1}(\mathbb{R}^3)$ with
$s>\frac{5}{2}$, there exists a
sufficient small $\varepsilon>0$ such that
if the initial velocity $u_{0}$ satisfies
\begin{align*}
\|u_0\|_{\dot{H}^{\frac{1}{2}}}<\varepsilon
\nu, \end{align*} and the initial
orientation $d_0$ satisfies \begin{align*}
\|d_0\|_{\dot{H}^{\frac{1}{2}}\cap\dot{H}^{\frac{3}{2}}}^2
\exp\Big\{
C\frac{\nu+\gamma}{\nu^2\gamma}(1+\|u_0\|_{\dot{H}^\frac{1}{2}}^2)\|
u_0\|_{\dot{H}^\frac{1}{2}}^2
\Big\}
<\varepsilon
\min\{\nu,\gamma\},
\end{align*} for some positive constant $C$, the
Ericksen-Leslie system admits a unique global solution
$(u,d)\in C(\mathbb{R}_+;
H^{s}(\mathbb{R}^3)\times
H^{s+1}(\mathbb{R}^3))$.
Combining continuous flow aerobic granulation using an external selector and carbon-efficient nutrient removal with AvN control in a full-scale simultaneous nitrification-denitrification process
Authors: Regmi Pusker , Sturm Belinda , Hiripitiyage Devinda , Keller Nancy , Murthy Sudhir , Jimenez Jose
Published: January 2024

The James R. Dolorio Water Reclamation Facility in Pueblo, Colorado,
uses AvN aeration controls to lower aeration energy while promoting
carbon-efficient nutrient removal and hydrocyclone-based wasting to
achieve SVI improvements and process intensification. The results from
the full-scale installation showed that hydrocyclone-based wasting
helped improve settling characteristics by reducing the SVI from 200 ±
52 mL/g to 83 ± 22 mL/g within weeks of operation. PAO and nitrifiers
were preferentially retained in dense flocs and granules, while lighter
heterotrophic and filamentous organisms were preferentially wasted, thus
uncoupling the SRT of these two fractions relative to overall SRT. The
SRT of dense aggregates was estimated at 14.4 ± 3.4 days and for lighter
flocs at 7.1 ± 2.3 days. The use of AvN control with continuous low DO
conditions resulted in low DO conditions (< 0.4 mgO2/L)
reducing air demands by 50% while providing excellent nitrogen
(effluent TIN < 11 mg/L) and TP removal (effluent TP
< 1 mg/L) at low primary effluent COD/N ratio of 6.0. The
presence of comammox was demonstrated through molecular analysis, while
ex-situ batch tests revealed the presence of DPAO, which could have
attributed to the energy and carbon-efficient biological nutrient
removal.
long non-coding RNA OIP5-AS1 suppresses microRNA-92a to augment proliferation and metastasis of ovarian cancer cells through upregulating ITGA6
Authors: Wang Yujue , Li Lingling , Zhang Xun , Zhao Xiaolan
Published: January 2024

Objective: Recently, long non-coding RNAs (lncRNAs) and microRNAs
(miRNAs) have been identified as essential biomarkers during development
of malignancies. This study was performed to study the roles of lncRNA
opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) and miR-92a
in ovarian cancer (OC). Methods: OIP5-AS1, miR-92a and integrin alpha 6
(ITGA6) expression in OC tissues and cells was assessed. The screened OC
cells were respectively with OIP5-AS1 silenced vector, miR-92a
mimic/inhibitor or depleted or restored integrin alpha 6 (ITGA6) vector.
The viability, migration, invasion and apoptosis of the cells were
determined and the levels of epithelial-mesenchymal transition
(EMT)-related proteins were also measured. The interactions between
OIP5-AS1 and miR-92a, and between miR-92a and ITGA6 were confirmed.
Results: OIP5-AS1 and ITGA6 were upregulated while miR-92a was
downregulated in OC. MiR-92a was a target of OIP5-AS1 and ITGA6 was
targeted by miR-92a. Inhibited OIP5-AS1 or ITGA6 or elevated miR-92a
repressed EMT, viability, migration and invasion, and promoted apoptosis
of OC cells. These effects that induced by silenced OIP5-AS1 could be
reversed by miR-92a inhibition while those that induced by up-regulated
miR-92a were reduced by restored ITGA6. Conclusion: OIP5-AS1 silencing
promoted miR-92a to repress proliferation and metastasis of OC cells
through inhibiting ITGA6.
Measuring coagulopathy in pediatric craniofacial surgery
Authors: Borst Alexandra , Bonfield Christopher , Deenadayalan Poornachanda , Le Chi , Xu Meng , Sobey Jenna , Reddy Srijaya
Published: January 2024

Complex cranial vault reconstruction (CCVR) for repair of
craniosynostosis is a procedure associated with high risk of bleeding
and resultant coagulopathy. The goal of this study was to describe lab
parameters in a cohort of pediatric patients undergoing CCVR and
identify if standard hematologic and coagulation laboratory results
could predict blood loss. We found that standard hematologic and
coagulation laboratory parameters predicted intraoperative and
postoperative blood loss, but provided limited mechanistic information
to improve our understanding of coagulopathy in craniofacial surgery.
Future laboratory-based studies would be useful in providing a
comprehensive model of coagulopathy in this population.
FACTORS AFFECTING POSTOPERATIVE RENAL FUNCTION IN NEPHRECTOMIES
Authors: Onay Meryem , BAŞ Sema ŞANAL , KURADA Gizem , KARAKOÇ Ebru , YELKEN Birgül
Published: January 2024

Introduction: Patients who have had a nephrectomy usually have a
previous history of renal dysfunction or are at risk for kidney failure
due to tissue loss during surgery. In this study, we aimed to
retrospectively evaluate the factors affecting acute renal function in
nephrectomy surgeries under sevoflurane anesthesia. Method: Demographic
data of patients, case type, surgery type, duration of anesthesia,
intraoperative lactate level, and postoperative complications of
patients who underwent nephrectomy in our clinic were reviewed. Renal
function was compared with the levels of urea, creatinine and estimated
glomerular filtration rate (eGFR) in the preoperative (2 day before
surgery) and postoperative period (day 2). Results: The mean age of the
patients 58.2 ± 13.02, duration of anesthesia 166.24 ± 53.01/min,
pre-operative eGFR 84.71 (67.69-90.0), and postoperative eGFR 65.09
(47.91-87.44) were respectively. When the pre- and postoperative renal
functions of the patients were compared, the statistically significant
results were those of urea (p: 0.01), creatinine (p:0.01), and GFR
(p:0.01). The factors affecting GFR decrease were age (p:0.01) and case
type (p:0.01). Conclusion: The incidence of acute renal failure is
increasing in nephrectomy surgeries, especially in radical ones. Age and
case type are effective in causing eGFR decrease.
Immunotherapy of Cancer by Targeting Regulatory T cells
Authors: Chen Bojin , Zhao Jing-Wen , Zhang Da-Hong , Zheng Ai-Hong , Wu Guo-Qing
Published: January 2024

Regulatory T (Treg) cells maintain immune homeostasis by inhibiting
abnormal/overactive immune responses to both autogenic and nonautogenic
antigens. Treg cells play an important role in immune tolerance,
autoimmune diseases, infectious diseases, organ transplantation, and
tumor diseases. Treg cells have two functional characteristics: T cell
anergy and immunosuppression. Treg cells remain immune unresponsive to
high concentrations of interleukin-2 and anti-CD3 monoclonal antibodies.
In addition, the activation of Treg cells after TCR-mediated signal
stimulation inhibits the activation and proliferation of effector T
cells. In the process of tumor development, Treg cells accumulate
locally in the tumor and lead to tumor escape by inducing anergy and
immunosuppression. It is believed that targeted elimination of Treg
cells can activate tumor-specific effector T cells and improve the
efficiency of cancer immunotherapy. Therefore, inhibition/clearance of
Treg cells is a promising strategy for enhancing antitumor immunity.
Here, we review studies of cancer immunotherapies targeting Treg cells.
Recent progress on tick-borne animal diseases in China
Authors: Jia Weijuan , Chen Si , Chi Shanshan , Yunjiang * , Ren Linzhu , Wang Xueli
Published: January 2024

Tick and Tick-borne diseases pose a growing threat to human and animal
health, which has brought great losses to livestock production. With the
continuous expansion of human activities and the development of natural
resources, there are more and more opportunities for the human to
contact ticks and tick-borne pathogens. Therefore, research on ticks and
tick-borne diseases is of great significance. This paper reviews recent
progress on tick-borne bacterial diseases, viral diseases, and parasitic
diseases in China, which provides a theoretical foundation for the
research of tick-borne diseases.
Increased frequency of circulating classical monocytes in patients with rosacea
Authors: Gao Cuie , Ge Lan , Chen Dewei , Zhang Mengjie , Zhao Li , Liu Wenying , Chen Shuguang , Wang Juan , Zhou Cunjian , Zhao Xingwang , Li Shifei , Song Zhiqiang , Li Jian
Published: January 2024

Monocyte subsets, including classical, intermediate and non-classical
monocytes, are involved in the pathogenesis of inflammatory or
autoimmune diseases. The pathogenic role of monocytes in the peripheral
blood mononuclear cells (PBMCs) of patients with rosacea remains
unclear. This study aimed to assess frequencies of monocyte subsets in
PBMCs from rosacea patients before and after clinical treatment. We
applied flow cytometry to examine frequencies of monocyte subsets in 116
patients with rosacea，while patients with 26 systemic lupus
erythematosus (SLE), 28 acne and 42 normal healthy subjects without skin
problems (HC) were recruited as controls. Expression of CCR2 on
monocytes and plasma levels of CCL2, HMGB-1, IL-1β and TNF-α were
measured in HC and rosacea patients before and after treatment. The
frequency of classical monocytes, but not intermediate or non-classical
monocytes, was higher in rosacea as compared with HC, which decreased
after treatment. Frequencies of monocyte subsets showed no gender
difference, while increased with age in patients but not in HC.
Frequencies of classical monocytes in patients with
erythromatotelangiectatic rosacea (ETR) and ETR-papulopaustula rosacea
(PPR) overlap were significantly higher than HC or patients with only
PPR or phytomatous rosacea (PhR). There was a significant higher
expression of CCR2 in classical monocytes, with higher plasma levels of
CCL2, HMGB-1, IL-1β and TNF-α in patients than in HC, which all
significantly decreased after treatment. Our data indicated a possible
association between abnormal classical monocytes frequencies and
rosacea.
Comparison of Clinical Characteristics in Adult Patients Under 65 years of age with and without Covid 19 pneumonia
Authors: Ozdemirel Tugce Sahin , Akkurt Esma Sevil , Ertan Ozlem , Gokler Mehmet Enes , Ozyurek Berna Akinci
Published: January 2024

Background: Coronavirus disease-2019 (COVID-19) can cause asymptomatic,
mild upper respiratory tract symptoms, pneumonia in young persons. How
the disease will progress in each patient is still unknown. Therefore,
we aimed to investigate the prognostic markers of the development of
pneumonia and the clinical characteristics of patients under 65 years
with COVID-19 confirmed by a positive reverse transcriptase polymerase
chain reaction (RT-PCR) test. Method: In this retrospective study, a
total of 271 patients admitted in our unit were included. The patients
were divided into two groups, those who did or did not develop
pneumonia. Their clinical features, treatment protocols and laboratory
parameters were recorded retrospectively. Results: Pneumonia developed
in 67.9% (n = 184) of the cases. Age in the pneumonia group was higher
than in the non-pneumonia group (p < 0.001). In the logistic
regression analysis, when symptom and comorbidity status were examined
according to the presence of pneumonia; HT (OR: 4,525 95% CL:
1,494–13,708) were the most important risk factor for pneumonia. When
age and laboratory values were examined according to the presence of
pneumonia, advanced age (OR: 1.042 95% CL: 1.01–1.073), low albumin
(OR: 0.917 95% CL: 0.854–0.986) and high troponin (OR: 1.291 95% CL:
1.044–1.596) were identified as risk factors for pneumonia. Conclusion:
HT, older age, low albumin, high troponin were important factors for
predicting COVID-19 pneumonia in patients under 65 years of age. Young
patients with these predictive factors should be more carefully
evaluated by further diagnostic procedures, such as thoracic CT. Key
Words: COVID-19, pneumonia, young adults
Patient Point of View of the diagnosis, treatment and follow up in acromegaly: Single center study from a tertiary center
Authors: Dirikoc Ahmet , Polat Burcak , genç birgül , keskin çağlar , Cakir Bekir
Published: January 2024

Objectives We aimed to evaluate the awareness and perspectives of
acromegaly patients in the diagnosis and treatment processes, as well as
to evaluate basic clinical and demographic features. Methods A
cross-sectional study was conducted at the Endocrinology Department of
Yildirim Beyazit University between March 2019-April 2020. Fifty-eight
acromegalic patients were enrolled. All patients were identified from
our database and called for a clinical visit and fill the questionnaire
forms. Results Fifty-eight patients were included in this study (41.4%
female). The mean age of the patients was 52 ± 10.8. Median years from
symptom to diagnosis (min-max) was 2 (1-12). 55.2% of the patients did
not graduate from high school. Thirty of fifty-eight (51.7%) patients
had knowledge about the etiology of their disease.While 12 patients
(20.7%) noticed their initial symptoms themselves, 75% of the patients
reported their symptoms during the clinical history taken by a health
care professional. The majority of patients were diagnosed by an
endocrinologist (69%). Acromegaly didn’t affect social life but
affected work life and caused early retirement. Transsphenoidal surgery
was performed as primary treatment in 96.6% of the patients (n=56). 46
patients (79.3%) received medical treatment with somatostatin receptor
ligands (SSRL) (octreotide or lanreotide LAR) with or without
cabergoline. Overall disease control was achieved in 38 patients
(65.5%). Conclusions Acromegaly is usually detected incidentally by
clinicians. The diagnosis of acromegaly is delayed in most patients and
disease-related complications have already developed at the time of
diagnosis. We think that increasing the awareness of the society and
health care professionals will reduce both disease-related comorbidities
and the economic burden on the health system.
A method for the analysis of African swine fever by viral metagenomic sequencing
Authors: Ji ChiHai , Jiang JingZhe , Wei Yingfang , Wang ZhiYuan , Chen Yongjie , Mai ZhanZhuo , Cai MengKai , Qin ChenXiao , Cai Yu , Yi HeYou , Liang Guan , Ji Chihai , Gong Lang , Zhang Guihong , Wang Heng
Published: January 2024

In 2018, there was an outbreak of African swine fever (ASF) in China,
which spread to other provinces in the following three years, severely
damaging China’s pig industry. ASF is caused by the African swine fever
virus (ASFV). Given that the genome of the African swine fever virus is
very complex and whole genome information is currently inadequate, it is
important to efficiently obtain virus genome sequences for genomic and
epidemiological studies. The prevalent ASFV strains have low genetic
variability; therefore, whole genome sequencing analysis provides a
basis for the study of ASFV. We provide a method for the efficient
sequencing of whole genomes, requiring only a small number of tissues.
The pig spleen were crushed and homogenized, and the tissue and cell
fragments were removed by low-speed centrifugation. The virus-like
particles (VLPs) in the solution were purified and concentrated by
filtration and over-speed centrifugation. After obtaining the relatively
pure VLP sample, nucleic acids were extracted simultaneously by
co-extraction of viral DNA (dsDNA, ssDNA) and RNA (ssRNA, dsRNA). Then,
the database construction method was selected according to the genomic
types of ASFV, and the whole ASFV genome was obtained through data
filtering, host sequence removal, virus classification, data assembly,
virus sequence identification, statistical analysis, gene prediction,
and functional analysis. Our proposed method will facilitate ASFV genome
sequencing and novel virus discovery.
The cyclic peptide OFP011 functioned as a multifunctional agonist at opioid/neuropeptide FF receptors with improved blood-brain barrier penetration
Authors: Fang Quan , Zhang Mengna , Xu Biao , Li Ning , Zhang Run , Zhang Qinqin , Chen Dan , Niu Jiandong , Xu Kangtai , Xiao Jian , Shi Yonghang
Published: January 2024

BACKGROUND AND PURPOSE A recently discovered multi-target
opioid/neuropeptide FF (NPFF) receptor agonist, DN-9, exhibited
peripherally acting analgesia. To improve its metabolic stability and
blood-brain barrier penetration, we designed and synthesized a novel
cyclic disulfide analogue of DN-9, OFP011, and examined its bioactivity
using in vitro cAMP and in vivo behavioral experiments. EXPERIMENTAL
APPROACH OFP011 was pharmacologically characterized using the in vitro
cAMP assays at opioid and NPFF receptors. The blood-brain barrier (BBB)
transport property of OFP011 was investigated using pharmacokinetic and
pharmacological experiments. Antinociceptive properties of OFP011 were
investigated in acute, inflammatory and neuropathic pain. Its adverse
effects on locomotor coordination, gastrointestinal transit,
antinociceptive tolerance, addiction, reward, and respiration were
further assessed. KEY RESULTS OFP011 exhibited multifunctional agonistic
efficacies at mu-, delta-, kappa-opioid, NPFF1 and NPFF2 receptors in
vitro. The pharmacokinetic and pharmacological experiments demonstrated
its BBB permeability after systemic administration. In addition,
subcutaneous OFP011 produced potent and long-lasting antinociception via
central opioid receptors in various pain models. At the highest
analgesic doses, subcutaneous OFP011 induced reduced adverse effects,
including tolerance, gastrointestinal transit, motor function,
addiction, reward, and respiration depression. Notably, OFP011 achieved
oral antinociceptive activity in different pain models. CONCLUSION AND
IMPLICATIONS OFP011 exhibited potent and sustained analgesia without
opioid-like side effects. Collectively, the results imply that the
multifunctional opioid/NPFF receptor agonists with improved BBB
penetration may be a promising strategy to treatment of moderate to
severe nociceptive and pathological pain with fewer side effects.
Microscopic polyangiitis presenting with persistent cough and hemoptysis in pediatrics: A case report and review
Authors: Zhu Yantong , chentao Liu , Zheng Xiangrong
Published: January 2024

Background：Microscopic polyangiitis (MPA) is a necrotizing vasculitis
which involves small- and medium-sized vessels. It is associated with
the presence of anti-neutrophil cytoplasmic antibodies with a
perinuclear staining pattern (p-ANCA). The kidney and lung are the
organs primarily affected. MPA is rare in children, and easily
misdiagnosed. In this work we describe a complete course of this
disease. Case presentation: An 11-year-old girl with a one-month cough
and hemoptysis, showed no improvement after imipenem-cilastatin
treatment. P-ANCA, and microscopic haematuria and proteinuria, were
positive, and a chest CT revealed an area of shadow in bilateral lower
lobes of the lungs. Renal biopsies revealed crescentic
glomerulonephritis. MPA was diagnosed based on these observations. The
patient showed dramatic clinical and imaging improvement after
immunosuppressive treatment. Conclusion: The organs and systems most
affected by MPA in children, are the lungs (about 80%), kidneys (about
80%), cutaneous involvement (about 20%), nervous system involvement
(about 16%), and gastrointestinal involvement (about 16%). Reasonable
care should be taken in examining those patients, while biopsies of the
kidney or any other of the organs remain the gold standard for
diagnostic purposes. Pulmonary involvement may be the initial symptom of
MPA, which is often misdiagnosed as pneumonia and antibiotics are used
at the beginning of the disease. Patients with hemoptysis should undergo
a comprehensive examination, including urine routine, to determine
whether there is renal involvement, and an immunological index detection
to determine whether it is an autoimmune disease.
The Application of Energy and Laplacian Energy of Hesitancy Fuzzy Graphs based on Similarity Measures in Decision-Making Problems
Authors: Shaik Sharief Basha , N Rajagopal Reddy
Published: January 2024

In this article, a new hesitancy fuzzy similarity measure is defined and
then used to develop the matrix of hesitancy fuzzy similarity measures,
which is subsequently used to classify hesitancy fuzzy graph using the
working procedure. We build a working procedure (Algorithm) for
estimating the eligible reputation scores values of experts by applying
hesitancy fuzzy preference relationships (HFPRs) and the usual
similarity degree of one distinct HFPRs to each other’s. As the last
step, we provide numerical examples to demonstrate and validate our
working procedure.
A survey of Iranian population attitude about vaccination against COVID-19 and evaluation of its spontaneous reporting of adverse effects
Authors: Namazi soha , mikaeili-mirak sevda , Ghadrdan elliyeh
Published: January 2024

Abstract Objectives: The COVID-19 pandemic continues to claim victims
around the world, and Iran is no exception from this ravage. Vaccine
development led to growing optimism regarding control of COVID-19.
Vaccine acceptance by the majority of the population is important for
the success in controlling COVID-19. This study assessed the attitude of
Iranian population toward COVID-19 vaccination. Also, the adverse
effects of the COVID-19 vaccines on vaccinated individuals were
reported. Method: This cross-sectional study was conducted from May to
July 2021 among Iranian population. The data was collected using the
online researcher-made questionnaire. The questionnaire contained 16
questions in 3 sections. Results: The questionnaire was filled out by
916 people in total. The majority of participants had 40-65 years old
(N:480, 52.4%). More than half of participants were from health care
team (N:543, 59.3%). The majority of participants trusted the vaccine
and said they would inject it (N:582, 63.5%). However more than 80% of
participants preferred non-domestic vaccines over Iranian ones.
Potential adverse effect following vaccination was the main concern of
responders (N:576, 62.9%). The most common adverse effects reported by
vaccine recipients were temporary fatigue and muscle pain (N:313,
71.95%). The level of education, academic area and area of practice in
the health system were significantly associated with the positive
response to COVID-19 vaccination (P-value 0.0001, 0.0001 and 0.004
respectively). Conclusion: According to the results of this study,
Iranian health authorities should provide clear information about the
safety and efficacy of COVID-19 vaccines, especially domestic types, to
increase public confidence and awareness regarding vaccines.