bluuebunny/crossref_metadata_2025 huggingface dataset:
Excommunication in Postrevolutionary England, 1689–1714
Authors: Jain Pranav
Published: February 2024

This article asks why many divines pushed for reform of the Church of England's use of excommunication after the Glorious Revolution of 1688. In response, it argues that, worried by what they perceived as widespread moral decline and the threat posed by the floodgates of Protestant dissent opened up by the Toleration Act of 1689, clergy became concerned that sentences such as excommunication were ineffective and the church would soon cease to be the chief arbiter of certain offenses. In contrast to existing historiography, this article suggests that the urge for reform was not confined to any particular section of the church. Instead, the reform of excommunication was a shared cause, although there was sharp disagreement about how to pursue it. However, despite enthusiasm for change, efforts for reform floundered because of partisan conflict and the legacy of the Tudor Reformation that continued to shape religious life in England well into the later Stuart period. Examining the debate about excommunication allows us to revise of our understandings of religion and politics in the last decades of the Stuart dynasty and further develop important concepts such as the long Reformation.
Resolvent-based motion-to-wake modelling of wind turbine wakes under dynamic rotor motion
Authors: Li Zhaobin , Yang Xiaolei
Published: February 2024

We propose a linearized deterministic model for predicting coherent structures in the wake of a floating offshore wind turbine subject to platform motions. The model's motion-to-wake predictive capability is achieved through two building blocks: a motion-to-forcing (M2F) part and a forcing-to-wake (F2W) part. The M2F model provides a unified framework to parameterize the effects of arbitrary floating wind turbine motions as unsteady loads of a fixed actuator disk, requiring only the radial distribution of the aerodynamics force coefficient on the blade as input. The F2W model is derived based on a bi-global resolvent model obtained from the linearized Navier–Stokes equations, using the time-averaged wake of a fixed wind turbine as input. In addition to its capability of predicting sensitive frequency ranges, the model excels linear stability analysis by providing spatial modes of the wake response in a motion-specific and phase-resolved manner. The model successfully predicts the wake pulsing mode induced by surge, as well as the similarity and difference of the wake meandering modes caused by sway and yaw. Large-eddy simulations under different inflow turbulence intensities (TIs) and length scales are further conducted to analyse the wake meandering triggered by the simultaneous excitation of free-stream turbulence and sway motion. The results show distinct frequency signatures for the wake dynamics induced by ambient turbulence and sway motion. The inflow TI is found to have a stabilizing effect on the wake, reducing the motion-induced wake responses. Such a stabilizing effect is captured satisfactorily with the proposed model, provided that the effective viscosity is calibrated properly using the data from the fixed turbine wake under the corresponding turbulent inflow.
High propulsive performance by an oscillating foil in a stratified fluid
Authors: Wang Jiadong , Kandel Prabal , Deng Jian
Published: February 2024

We investigate numerically the propulsion characteristics of an oscillating foil undergoing coupled heave and pitch motion in a linearly density-stratified flow. A parameter space defined by the internal Froude number (

$1 \le Fr \le 10$

) and the maximum angle of attack (

$5^\circ \le {\alpha _0} \le 30^\circ$

) is considered in our study. The results demonstrate a significant enhancement in both thrust production and propulsive efficiency due to the stratification influence. Notably, the highest efficiency exceeding

$80\,\%$

is achieved under moderate stratification conditions, surpassing the performance observed in a homogeneous fluid. We attribute this optimum performance to the proper match between the stratification effect and foil kinematics, which gives rise to intense vortex interactions and sufficient wave–mean flow interactions in the near wake of the oscillating foil. Consequently, the energy is transferred towards wake structures to form a high-intensity momentum jet in close proximity to the foil's trailing edge, indicating efficient propulsion. Furthermore, we find that the stratifications within the moderate-to-strong transitional regime display a reduced dependence of propulsive efficiency on the maximum angle of attack, primarily due to the delaying and alleviating effects on dynamic-stall events. Such a mechanism enables the oscillating foil to maintain a satisfactory performance by sufficiently high angles of attack without the penalty of stall events. Based on our findings, we propose that animals or artificial vehicles utilising oscillatory propulsion can benefit from the presence of density stratification in the surrounding fluid.
Recent change in surface mass-balance trends of glaciers on James Ross Island, north-eastern Antarctic Peninsula
Authors: Engel Zbyněk , Láska Kamil , Smolíková Jana , Kavan Jan
Published: February 2024

Glaciers cover 132 900 km2 around the Antarctic Ice Sheet, but few are subject to annual mass-balance measurements. Lookalike Glacier and Davies Dome on James Ross Island have been monitored since 2009, providing the third longest mass-balance record for the northern Antarctic Peninsula. These glaciers had a balanced mass budget over the period 2009/10–2014/15 but started to lose their mass thereafter. Between 2014/15 and 2020/21, mass change rates were −0.15 ± 0.13 and −0.26 ± 0.11 m w.e. a−1 for Lookalike Glacier and Davies Dome, respectively. The mean equilibrium-line altitudes over this period at Lookalike Glacier (362 ± 18 m a.s.l.) and Davies Dome (>427 ± 22 m a.s.l.) are 51 and >34 m higher compared to the previous 6-year period. The mean accumulation area ratio values determined for the period 2014/15–2020/21 are lower than the balanced-budget ratio indicating that glaciers are out of balance with the current climate. The data confirm the transition from positive to negative mass-balance periods around 2014/15, which is attributed to the change in air temperature trends. The mean summer temperature increased by 0.9°C between the periods 2009/10–2014/15 and 2015/16–2020/21 and melt-season temperatures became predominantly positive.
Cisuralian–Guadalupian brachiopod assemblages from the northern Tengchong Block in western Yunnan, China and their paleogeographical implications—a revisit
Authors: Zong Pu , Zhan Lipei , Lee Sangmin , Huang Hao , Shi G.R. , Jin Xiaochi
Published: February 2024

The early to middle Permian brachiopods from the Tengchong Block in western Yunnan, southwestern China, play important roles in biostratigraphic correlation and paleogeographic inferences of tectonic units on eastern peri-Gondwana. However, detailed taxonomic studies of these brachiopods have been limited. In this paper, we provide the systematic description of three Permian brachiopod assemblages from the Shanmutang section in the northern Tengchong Block, which in ascending order include theElivina-EtherilosiaAssemblage from the top of the Kongshuhe Formation, and theSpiriferella-SpiriferellinaAssemblage and theWaagenites-CostiferinaAssemblage from the base and lower part, respectively, of the overlying Dadongchang Formation. Based on the biostratigraphic assessment of the brachiopod taxa as well as the age constraints from the associated fusulinid assemblages, the age of theElivina-EtherilosiaAssemblage is considered to be most likely late Sakmarian to early Artinskian, theSpiriferella-SpiriferellinaAssemblage probably late Artinskian, and theWaagenites-CostiferinaAssemblage late Roadian to early Wordian. A transition from cool-water faunas with Gondwanan affinities to mixed warm-temperate (transitional) faunas with Cathaysian elements is evident for the Artinskian and through the Guadalupian. The climatic transition inferred from the faunal evidence is interpreted to have resulted from the combined effect of Gondwana deglaciation and the northward drift of the peri-Gondwanan blocks (including the Tengchong Block) during the Cisuralian and Guadalupian.
What does Covid-19 teach us about English contract law?
Authors: Mitchell Catherine
Published: February 2024

This paper examines how English courts have responded to the contract problems generated by the Covid-19 pandemic and considers what this tells us about future contract law development. In relation to consumers, the case law on pandemic-affected contracts, though limited, indicates that traditional contract doctrine does not necessarily produce beneficial outcomes for consumers. This further diminishes the importance of the common law in the consumer contracting context. In the commercial sector, contracting parties were encouraged by government and other organisations to co-operate with one another and act in good faith during the crisis, but this has not influenced the courts applying contract law in the pandemic aftermath. The emerging case law suggests that contract law has retained its commitment to certainty, freedom of contract and sanctity of contract, notwithstanding the extraordinary circumstances around the outbreak and its unpredictable effects on contracts. The unalloyed application of formal contract law in the post-pandemic case law augments the position of relational norms as extra-contractual in English law, putting the further judicial development of relational contract principles in doubt. The paper concludes that despite the considerable social and economic upheaval caused by the pandemic, its impact on contract law development is likely to be minimal.
A New Response to the Problem of Natural Evil
Authors: Cumberland Caleb
Published: February 2024

This paper will offer a new defense in response to the problem of natural evil, called the Perfect Will defense. The defense argues that in sustaining the universe, God conforms the system of physical laws to his intellect and will. Yet, God could not fully conform the system of laws (for our universe) to his intellect and will without simultaneously forcing people into a loving relation with God. Yet, since God would not force people to love him, God must thereby initially create people in a universe that has a system of laws that is only partially conformed to God’s intellect and will. However, while a universe with a system of laws that is only partially conformed to God’s intellect and will allows for people to exercise their freedom over their relation with God, it also results in the occurrence of natural evils. The paper will argue that once this defense is fully developed, it is able to account for why God allows for natural evils to occur within the universe. The paper will outline the defense, as well as respond to the defense’s major objections.
Using paleopathology to provide a deep-time perspective that improves our understanding of one health challenges: Exploring urbanization
Authors: Mitchell Piers D.
Published: February 2024

The question posed is how deep-time perspectives contribute to tackling contemporary One Health challenges, improving understanding and disease mitigation. Using evidence from the field of paleopathology, it is possible to explore this question and highlight key learning points from the past to focus the minds of those making healthcare policy decisions today. In previous centuries urbanization led to poorer health for a wide range of indicators, including life expectancy, sanitation and intestinal parasites, airway disorders such as maxillary sinusitis, metabolic diseases such as rickets, and even conditions resulting from clothing fashions such as bunions. Modern concerns regarding the quality of urban air and rivers show we have still to incorporate these lessons. When we consider major infectious diseases affecting past societies such as bubonic plague, tuberculosis and leprosy, interaction between humans and wild mammal reservoirs was key. Wild red squirrels in Britain today remain infected by the medieval strain of leprosy that affected people 1,500 years ago. It is clear that the One Health focus on the interaction between humans, animals and their environment is important. Eradicating zoonotic infectious diseases from humans but not these reservoirs leaves the door open to their spread back to people in the future.
Analysis of insecticide resistance and de novo transcriptome assembly of resistance associated genes in the European grapevine moth, Lobesia botrana (Lepidoptera: Tortricidae)
Authors: Albaz Esra , Katsavou Evangelia , Cagatay Naciye Sena , Ioannidis Panagiotis , Ilias Aris , Mylona Kyriaki , Kremi Katerina , Roditakis Emmanouil , Guz Nurper , Vontas John
Published: February 2024

The European grapevine moth Lobesia botrana (Denis & Shiffermüller 1776) is an economically important pest of the vine-growing areas worldwide. Chemical insecticides have been used for its control; however, its resistance status is largely unknown in many regions. We monitored the susceptibility of several L. botrana populations from Greece and Turkey. In addition, based on RNAseq transcriptome analysis, we identified and phylogenetically classify the cytochrome P450 genes of L. botrana, as well as analysed target site sequences and looked for the presence of known resistance mutations. Resistance against chlorantraniliprole, alpha-cypermethrin, spinetoram, etofenprox, and acetamiprid was very low (below 2.5-fold in all cases, compared to a reference strain from Greece) in all populations from Greece that were included in the study. However, resistance against indoxacarb (4–30-fold), spinosad (5–59-fold), and deltamethrin (18–30 fold) was detected in the L. botrana populations from Turkey, compared to a reference population from Turkey. De novo transcriptome assembly and manual annotation, and subsequent PCR-based analysis of insecticide target sequences (i.e. voltage-gated sodium channel – VGSC: target of pyrethroids and oxadiazines; nicotinic acetylcholine receptor subunit a6 – nAChR_α6: target of spinosad; ryanodine receptor – RyR: target of diamides; glutamate-gated chloride channel – GluCl: target of avermectins and; acetylcholinesterase – AChE: target of organophosphates) showed the absence of known resistance mutations in all specimens from both countries. Finally, the L. botrana CYPome (116 genes) was manually analysed and phylogenetically characterised, to provide resources for future studies that will aim the analysis of metabolic resistance.
The Anarchy of Children's Archives: Citizenship and Empire in the Global 1930s
Authors: MURPHY EMILY
Published: February 2024

This article considers how the archive, particularly material produced by children, destabilizes the boundaries between the domestic and the foreign, citizenship and empire. Through its analysis of a wave of educational reform in the United States during the 1930s, which encouraged global citizenship among the young, it demonstrates how children not typically associated with global citizenship – those from both rural and working-class backgrounds – engaged with the imperial messages embedded in global education of the period.
Laser-machined two-stage nozzle optimised for laser wakefield acceleration
Authors: Tomkus V. , Mackevičiūtė M. , Dudutis J. , Girdauskas V. , Abedi-Varaki M. , Gečys P. , Račiukaitis G.
Published: February 2024

In this paper, the modelling and manufacturing of a two-stage supersonic gas jet nozzle enabling the formation of adaptive plasma concentration profiles for injection and acceleration of electrons using few-cycle laser beams are presented. The stages are modelled using the rhoSimpleFoam algorithm of the OpenFOAM computational fluid dynamics software. The first 200–300

${\rm \mu}$

m diameter nozzle stage is dedicated to 1 % N2 + He gas jet formation and electron injection. By varying the pressure between the first and second stages of the injectors, the electron injection location could be adjusted, and the maximum acceleration distance could be ensured. By changing the concentration of the nitrogen in the gas mixture, the charge of the accelerated electrons could be controlled. The second nozzle stage is designed for acceleration in fully ionised He or hydrogen gas and forms the optimal plasma concentration for bubble formation depending on the laser pulse energy, duration and focused beam diameter. In order to reduce the diameter of the plasma profile formed by the first nozzle and the concentration drop gap between the two nozzles, a one-side straight section was introduced in the first nozzle. The shock wave reflected from the straight section of the wall propagates parallel to the shock wave of the intersecting supersonic jets and ensures a minimal gap between the jets. The second-stage longitudinal plasma concentration profile could have an increasing gas density gradient to compensate for dephasing between the electron bunch and the plasma wave due to wave shortening with increasing plasma concentration.
How to be absolutely fair Part I: The Fairness formula
Authors: Wintein Stefan , Heilmann Conrad
Published: February 2024

We present the first comprehensive theory of fairness that conceives of fairness as having two dimensions: a comparative and an absolute one. The comparative dimension of fairness has traditionally been the main interest of Broomean fairness theories. It has been analysed as satisfying competing individual claims in proportion to their respective strengths. And yet, many key contributors to Broomean fairness agree that ‘absolute’ fairness is important as well. We make this concern precise by introducing the Fairness formula and the absolute priority rule and analyse their implications for comparative fairness.
Emerging evidence on selenoneine and its public health relevance in coastal populations: a review and case study of dietary Se among Inuit populations in the Canadian Arctic
Authors: Little Matthew , Achouba Adel , Ayotte Pierre , Lemire Mélanie
Published: February 2024

Selenium is an essential mineral yet both deficiency and excess are associated with adverse health effects. Dietary intake of Se in humans varies greatly between populations due to food availability, dietary preferences, and local geological and ecosystem processes impacting Se accumulation into agricultural products and animal populations. We argue there is a need to evaluate and reconsider the relevance of public health recommendations on Se given recent evidence, including the metabolic pathways and health implications of Se. This argument is particularly pertinent for Inuit populations in Northern Canada, who often exceed dietary tolerable upper intake levels and exhibit very high whole blood Se concentrations due to their dependence on local country foods high in the newly discovered Se compound, selenoneine. Since selenoneine appears to have lower toxicity compared to other Se species and does not contribute to the circulating pools of Se for selenoprotein synthesis, we argue that total dietary Se or total Se in plasma or whole blood are poor indicators of Se adequacy for human health in these populations. Overall, this review provides an overview of the current evidence of Se speciation, deficiency, adequacy, and excess and implications for human health and dietary recommendations, with particular reference to Inuit populations in the Canadian Arctic and other coastal populations consuming marine foods.
Optimal pattern synthesis of circular antenna arrays with improved effective aperture and beam area
Authors: Kumar Satish , Ram Gopi , Mandal Durbadal , Kar Rajib
Published: February 2024

Circular antenna array (CAA) is one of the most widely used antenna array designs. This paper addresses the design challenges of the CAA with the non-uniform single ring, which is placed in an X-Y plane with the best sidelobe level (SLL) and improved first null beamwidth (FNBW). It has been solved using differential evolution, craziness-based particle swarm optimization (CRPSO), and novel particle swarm optimization (NPSO) techniques. An optimal combination of feeding current and inter-element spacing provides an array pattern with the best SLL and improved FNBW, as well as some other parameter calculations of the antenna array like maximum directivity, maximum effective aperture, total effective aperture, maximum beam area, total beam area, circumference, and radius of the CAAs using these techniques. There are six designs of CAAs with different antenna elements (i.e., 10-, 12-, 16-, 20-, 36-, and 64-elements) which have been taken into account. Simulations are done in MATLAB. Based on various simulation results, we can analyze the performance of SLL and FNBW with other parameters using NPSO and compare them with different techniques of CAAs, as shown in the numerical analysis and simulation result section.
A review of existing model-based scenarios achieving SDGs: progress and challenges
Authors: Orbons K. , van Vuuren D.P. , Ambrosio G. , Kulkarni S. , Weber E. , Zapata V. , Daioglou V. , Hof A.F. , Zimm C.
Published: February 2024

Non-Technical Summary

In 2015, the United Nations articulated the ambition to move toward a prosperous, socially inclusive, and environmentally sustainable future for all by adopting the Sustainable Development Goals (SDGs). However, little is known about the pathways that could lead to their concurrent achievement. We provide an overview of the current literature on quantitative pathways toward the SDGs, indicate the commonly used methods and indicators, and identify the most comprehensive pathways that have been published to date. Our results indicate that there is a need for more scenarios toward the full set of SDGs, using a wider range of underlying narratives.

Technical Summary

Quantitative goal-seeking scenario studies could help to explore the needed systems' transformations to implement the 2030 Agenda for Sustainable Development by identifying enabling conditions and accounting for the synergies and trade-offs between the SDGs. Given that the SDGs were adopted some time ago, here, we review the existing global scenario literature to determine what it can offer in this context. We found only a few scenarios that address a large set of SDGs, while many more deal with specific clusters of 2–6 SDGs. We identified the most frequent clusters and compared the results of the most comprehensive sustainable development scenarios. The latter is complicated because of the diversity of methods, indicators, and assumptions used. Therefore, we suggest that an effort is needed to develop a wider set of scenarios that would achieve multiple SDGs, using a more standardized framework of targets and indicators.

Social Media Summary

This study reviews the current global pathways toward the SDGs and shows the need for a broader set of SDG scenarios.
On the Two Approaches to Incorporate Wave‐Particle Resonant Effects Into Global Test Particle Simulations
Authors: Lukin A. S. , Artemyev A. V. , Zhang X.‐J. , Allanson O. , Tao X.
Published: February 2024

Energetic electron dynamics in the Earth's radiation belts and near‐Earth plasma sheet are controlled by multiple processes operating on very different time scales: from storm‐time magnetic field reconfiguration on a timescale of hours to individual resonant wave‐particle interactions on a timescale of milliseconds. The most advanced models for such dynamics either include test particle simulations in electromagnetic fields from global magnetospheric models, or those that solve the Fokker‐Plank equation for long‐term effects of wave‐particle resonant interactions. The most prospective method, however, would be to combine these two classes of models, to allow the inclusion of resonant electron scattering into simulations of electron motion in global magnetospheric fields. However, there are still significant outstanding challenges that remain regarding how to incorporate the long term effects of wave‐particle interactions in test‐particle simulations. In this paper, we describe in details two approaches that incorporate electron scattering in test particle simulations: stochastic differential equation (SDE) approach and the mapping technique. Both approaches assume that wave‐particle interactions can be described as a probabilistic process that changes electron energy, pitch‐angle, and thus modifies the test particle dynamics. To compare these approaches, we model electron resonant interactions with field‐aligned whistler‐mode waves in dipole magnetic fields. This comparison shows advantages of the mapping technique in simulating the nonlinear resonant effects, but also underlines that more significant computational resources are needed for this technique in comparison with the SDE approach. We further discuss applications of both approaches in improving existing models of energetic electron dynamics.
A Cold Lid on a Warm Ocean: Indian Ocean Surface Rain Layers and Their Feedbacks to the Atmosphere
Authors: Shackelford Kyle , DeMott Charlotte A. , Jan van Leeuwen Peter , Mazloff Matthew R. , Sun Rui
Published: February 2024

Ocean surface rain layers (RLs) form when relatively colder, fresher, less dense rain water stably stratifies the upper ocean. RLs cool sea surface temperature (SST) by confining surface evaporative cooling to a thin near‐surface layer, and generate sharp SST gradients between the cool RL and the surrounding ocean. In this study, ocean‐atmosphere coupled simulations of the November 2011 Madden‐Julian Oscillation (MJO) event are conducted with and without RLs to evaluate two pathways for RLs to influence the atmosphere. The first, termed the “SST gradient effect,” arises from the hydrostatic adjustment of the boundary layer to RL‐enhanced SST gradients. The second, termed the “SST effect,” arises from RL‐induced SST reductions impeding the development of deep atmospheric convection. RLs are found to sharpen SST gradients throughout the MJO suppressed and suppressed‐to‐enhanced convection transition phases, but their effect on convection is only detected during the MJO suppressed phase when RL‐induced SST gradients enhance low‐level convergence/divergence and broaden the atmospheric vertical velocity probability distribution below 5 km. The SST effect is more evident than the SST gradient effect during the MJO transition phase, as RLs reduce domain average SST by 0.03 K and narrow vertical velocity distribution, thus delaying onset of deep convection. A delayed SST effect is also identified, wherein frequent RLs during the MJO transition phase isolate accumulated subsurface ocean heat from the atmosphere. The arrival of strong winds at the onset of the MJO active phase erodes RLs and releases subsurface ocean heat to the atmosphere, supporting the development of deep convection.
Seasonal Wetlands Make a Relatively Limited Contribution to the Dissolved Carbon Pool of a Lowland Headwater Tropical Stream
Authors: Solano Vanessa , Duvert Clément , Hutley Lindsay B. , Cendón Dioni I. , Maher Damien T. , Birkel Christian
Published: February 2024

Wetlands process large amounts of carbon (C) that can be exported laterally to streams and rivers. However, our understanding of wetland inputs to streams remains unclear, particularly in tropical systems. Here we estimated the contribution of seasonal wetlands to the C pool of a lowland headwater stream in the Australian tropics. We measured dissolved organic and inorganic C (DOC and DIC) and dissolved gases (carbon dioxide—CO2, methane—CH4) during the wet season along the mainstem and in wetland drains connected to the stream. We also recorded hourly measurements of dissolved CO2 along a ‘stream–wetland drain–stream’ continuum, and used a hydrological model combined with a simple mass balance approach to assess the water, DIC and DOC sources to the stream. Seasonal wetlands contributed ∼15% and ∼16% of the DOC and DIC loads during our synoptic sampling, slightly higher than the percent area (∼9%) they occupy in the catchment. The riparian forest (75% of the DOC load) and groundwater inflows (58% of the DIC load) were identified as the main sources of stream DOC and DIC. Seasonal wetlands also contributed marginally to stream CO2 and CH4. Importantly, the rates of stream CO2 emission (1.86 g C s−1) and DOC mineralization (0.33 g C s−1) were much lower than the downstream export of DIC (6.39 g C s−1) and DOC (2.66 g g C s−1). This work highlights the need for further research on the role of riparian corridors as producers and conduits of terrestrial C to tropical streams.
Climate Scientist Michael Mann Confronts Defamers in Court After 12-Year Delay and Wins
Authors: Cartier Kimberly
Published: February 2024

The trial comes as climate impacts and attacks on science and its practitioners are worse than ever.
Graft-versus-host-disease prophylaxis with ATG or PTCY in patients with lymphoproliferative disorders undergoing reduced intensity conditioning regimen HCT from one antigen mismatched unrelated donor
Authors: Paviglianiti Annalisa , Ngoya Maud , Peña Marta , Boumendil Ariane , Gülbas Zafer , Ciceri Fabio , Bonifazi Francesca , Russo Domenico , Fegueux Nathalie , Stolzel Friedrich , Bulabois Claude Eric , Socié Gerard , Forcade Edouard , Solano Carlo , Finel Hervé , Robinson Stephen , Glass Bertram , Montoto Silvia
Published: February 2024

Post-transplant cyclophosphamide (PTCY) has been introduced as graft-versus-host disease (GvHD) prophylaxis in mismatched and matched unrelated hematopoietic cell transplant (HCT). However, data comparing outcomes of PTCY or ATG in patients undergoing a 1 antigen mismatched HCT for lymphoproliferative disease are limited. We compared PTCY versus ATG in adult patients with lymphoproliferative disease undergoing a first 9/10 MMUD HCT with a reduced intensity conditioning regimen from 2010 to 2021. Patients receiving PTCY were matched to patients receiving ATG according to: age, disease status at transplant, female to male matching, stem cell source and CMV serology. Grade II-IV acute GvHD at 100 day was 26% and 41% for the ATG and PTCY group, respectively (p = 0.08). Grade III–IV acute GvHD was not significantly different between the two groups. No differences were observed in relapse incidence, non-relapse mortality, progression-free survival, overall survival and GvHD-relapse-free survival at 1 year. The cumulative incidence of 1-year extensive chronic GvHD was 18% in the ATG and 5% in the PTCY group, respectively (p = 0.06). In patients with lymphoproliferative diseases undergoing 9/10 MMUD HCT, PTCY might be a safe option providing similar results to ATG prophylaxis. Due to the limited number of patients, prospective randomized trials are needed.
Hematopoietic stem cell transplantation for pediatric patients with non-anaplastic peripheral T-cell lymphoma. An EBMT pediatric diseases working party study
Authors: Moser Olga , Ngoya Maud , Galimard Jacques-Emmanuel , Dalissier Arnaud , Dalle Jean Hugues , Kalwak Krzysztof , Wössmann Wilhelm , Burkhardt Birgit , Bierings Marc , Gonzalez-Vicent Marta , López Corral Lucía , Mellgren Karin , Attarbaschi Andishe , Bourhis Jean Henri , Carlson Kristina , Corbacioglu Selim , Drabko Katarzyna , Sundin Mikael , Toporski Jacek , Cario Gunnar , Kontny Udo
Published: February 2024

Peripheral T-cell lymphomas (PTCL) other than anaplastic large-cell lymphoma are rare in children, and the role of hematopoietic stem cell transplantation (HSCT) has not been clarified yet. In a retrospective analysis of registry-data of the European Society for Blood and Marrow Transplantation we analyzed 55 patients aged < 18 years who received allogeneic (N = 46) or autologous (N = 9) HSCT for PTCL. Median age at HSCT was 13.9 years; 33 patients (60%) were in first remission, and 6 (19%) in progression at HSCT. Conditioning was myeloablative in 87% of the allogeneic HSCTs and in 27 (58.7%) based on total body irradiation. After allogeneic HSCT the 5-year overall- and progression-free survival was 58.9% (95% CI 42.7–71.9) and 52.6% (95% CI 36.8–66.1), respectively. 5-year relapse incidence was 27.6% (95% CI 15.1–41.6), the non-relapse mortality rate was 19.8% (95% CI 9.7–32.6). Five of the six patients with progression at HSCT died. Seven of nine patients after autologous HSCT were alive and disease-free at last follow-up. Our data suggest a role of allogeneic HSCT in consolidation-treatment of patients with high-risk disease, who reach at least partial remission after primary- or relapse-therapy, whereas patients with therapy-refractory or progressive disease prior to transplantation do not profit from HSCT.
Convenient synthesis and delivery of a megabase-scale designer accessory chromosome empower biosynthetic capacity
Authors: Ma Yuan , Su Shuxin , Fu Zongheng , Zhou Chu , Qiao Bin , Wu Yi , Yuan Ying-Jin
Published: February 2024

Synthetic biology confers new functions to hosts by introducing exogenous genetic elements, yet rebuilding complex traits that are based on large-scale genetic information remains challenging. Here, we developed a CRISPR/Cas9-mediated haploidization method that bypasses the natural process of meiosis. Based on the programmed haploidization in yeast, we further developed an easy-to-use method designated HAnDy (Haploidization-based DNA Assembly and Delivery in yeast) that enables efficient assembly and delivery of large DNA, with no need for any fussy in vitro manipulations. Using HAnDy, a de novo designed 1.024 Mb synthetic accessory chromosome (synAC) encoding 542 exogenous genes was parallelly assembled and then directly transferred to six phylogenetically diverse yeasts. The synAC significantly promotes hosts’ adaptations and increases the scope of the metabolic network, which allows the emergence of valuable compounds. Our approach should facilitate the assembly and delivery of large-scale DNA for expanding and deciphering complex biological functions.
Novel variant of FBN2 in a patient with congenital contractual arachnodactyly
Authors: Nakama Mina , Miwa Yuki , Manabe Sayaka , Shimamoto Shigeru , Ohnishi Hidenori
Published: February 2024

Congenital contractual arachnodactyly (CCA) is a genetic connective tissue disorder that is characterized by arachnodactyly, kyphoscoliosis, marfanoid habitus, and crumpled ears. We report a case of a boy with suspected Marfan syndrome. Genetic analysis revealed c.3207_3217+9del in a heterozygote form of the fibrillin-2 (FBN2) gene. This patient was diagnosed with CCA based on his phenotype, and the pathogenicity of this variant was classified according to cDNA analysis and protein modeling.
Re-evaluation of battery-grade lithium purity toward sustainable batteries
Authors: Choe Gogwon , Kim Hyungsub , Kwon Jaesub , Jung Woochul , Park Kyu-Young , Kim Yong-Tae
Published: February 2024

Recently, the cost of lithium-ion batteries has risen as the price of lithium raw materials has soared and fluctuated. Notably, the highest cost of lithium production comes from the impurity elimination process to satisfy the battery-grade purity of over 99.5%. Consequently, re-evaluating the impact of purity becomes imperative for affordable lithium-ion batteries. In this study, we unveil that a 1% Mg impurity in the lithium precursor proves beneficial for both the lithium production process and the electrochemical performance of resulting cathodes. This is attributed to the increased nucleation seeds and unexpected site-selective doping effects. Moreover, when extended to an industrial scale, low-grade lithium is found to reduce production costs and CO2 emissions by up to 19.4% and 9.0%, respectively. This work offers valuable insights into the genuine sustainability of lithium-ion batteries.
Melt-quenched carboxylate metal–organic framework glasses
Authors: Kim Minhyuk , Lee Hwa-Sub , Seo Dong-Hyun , Cho Sung June , Jeon Eun-chae , Moon Hoi Ri
Published: February 2024

Although carboxylate-based frameworks are commonly used architectures in metal-organic frameworks (MOFs), liquid/glass MOFs have thus far mainly been obtained from azole- or weakly coordinating ligand-based frameworks. This is because strong coordination bonds of carboxylate ligands to metals block the thermal vitrification pathways of carboxylate-based MOFs. In this study, we present the example of carboxylate-based melt-quenched MOF glasses comprising Mg2+ or Mn2+ with an aliphatic carboxylate ligand, adipate. These MOFs have a low melting temperature (Tm) of 284 °C and 238 °C, respectively, compared to zeolitic-imidazolate framework (ZIF) glasses, and superior mechanical properties in terms of hardness and elastic modulus. The low Tm may be attributed to the flexibility and low symmetry of the aliphatic carboxylate ligand, which raises the entropy of fusion (ΔSfus), and the lack of crystal field stabilization energy on metal ions, reducing enthalpy of fusion (ΔHfus). This research will serve as a cornerstone for the integration of numerous carboxylate-based MOFs into MOF glasses.
Targeting DNA2 overcomes metabolic reprogramming in multiple myeloma
Authors: Thongon Natthakan , Ma Feiyang , Baran Natalia , Lockyer Pamela , Liu Jintan , Jackson Christopher , Rose Ashley , Furudate Ken , Wildeman Bethany , Marchesini Matteo , Marchica Valentina , Storti Paola , Todaro Giannalisa , Ganan-Gomez Irene , Adema Vera , Rodriguez-Sevilla Juan Jose , Qing Yun , Ha Min Jin , Fonseca Rodrigo , Stein Caleb , Class Caleb , Tan Lin , Attanasio Sergio , Garcia-Manero Guillermo , Giuliani Nicola , Berrios Nolasco David , Santoni Andrea , Cerchione Claudio , Bueso-Ramos Carlos , Konopleva Marina , Lorenzi Philip , Takahashi Koichi , Manasanch Elisabet , Sammarelli Gabriella , Kanagal-Shamanna Rashmi , Viale Andrea , Chesi Marta , Colla Simona
Published: February 2024

DNA damage resistance is a major barrier to effective DNA-damaging therapy in multiple myeloma (MM). To discover mechanisms through which MM cells overcome DNA damage, we investigate how MM cells become resistant to antisense oligonucleotide (ASO) therapy targeting Interleukin enhancer binding factor 2 (ILF2), a DNA damage regulator that is overexpressed in 70% of MM patients whose disease has progressed after standard therapies have failed. Here, we show that MM cells undergo adaptive metabolic rewiring to restore energy balance and promote survival in response to DNA damage activation. Using a CRISPR/Cas9 screening strategy, we identify the mitochondrial DNA repair protein DNA2, whose loss of function suppresses MM cells’ ability to overcome ILF2 ASO−induced DNA damage, as being essential to counteracting oxidative DNA damage. Our study reveals a mechanism of vulnerability of MM cells that have an increased demand for mitochondrial metabolism upon DNA damage activation.
Redox-tunable isoindigos for electrochemically mediated carbon capture
Authors: Li Xing , Zhao Xunhua , Zhang Lingyu , Mathur Anmol , Xu Yu , Fang Zhiwei , Gu Luo , Liu Yuanyue , Liu Yayuan
Published: February 2024

Efficient CO2 separation technologies are essential for mitigating climate change. Compared to traditional thermochemical methods, electrochemically mediated carbon capture using redox-tunable sorbents emerges as a promising alternative due to its versatility and energy efficiency. However, the undesirable linear free-energy relationship between redox potential and CO2 binding affinity in existing chemistry makes it fundamentally challenging to optimise key sorbent properties independently via chemical modifications. Here, we demonstrate a design paradigm for electrochemically mediated carbon capture sorbents, which breaks the undesirable scaling relationship by leveraging intramolecular hydrogen bonding in isoindigo derivatives. The redox potentials of isoindigos can be anodically shifted by >350 mV to impart sorbents with high oxygen stability without compromising CO2 binding, culminating in a system with minimised parasitic reactions. With the synthetic space presented, our effort provides a generalisable strategy to finetune interactions between redox-active organic molecules and CO2, addressing a longstanding challenge in developing effective carbon capture methods driven by non-conventional stimuli.
The role of interfacial donor–acceptor percolation in efficient and stable all-polymer solar cells
Authors: Wang Zhen , Guo Yu , Liu Xianzhao , Shu Wenchao , Han Guangchao , Ding Kan , Mukherjee Subhrangsu , Zhang Nan , Yip Hin-Lap , Yi Yuanping , Ade Harald , Chow Philip C. Y.
Published: February 2024

Polymerization of Y6-type acceptor molecules leads to bulk-heterojunction organic solar cells with both high power-conversion efficiency and device stability, but the underlying mechanism remains unclear. Here we show that the exciton recombination dynamics of polymerized Y6-type acceptors (Y6-PAs) strongly depends on the degree of aggregation. While the fast exciton recombination rate in aggregated Y6-PA competes with electron-hole separation at the donor–acceptor (D–A) interface, the much-suppressed exciton recombination rate in dispersed Y6-PA is sufficient to allow efficient free charge generation. Indeed, our experimental results and theoretical simulations reveal that Y6-PAs have larger miscibility with the donor polymer than Y6-type small molecular acceptors, leading to D–A percolation that effectively prevents the formation of Y6-PA aggregates at the interface. Besides enabling high charge generation efficiency, the interfacial D–A percolation also improves the thermodynamic stability of the blend morphology, as evident by the reduced device “burn-in” loss upon solar illumination.
Glucocorticoids increase adiposity by stimulating Krüppel-like factor 9 expression in macrophages
Authors: Zhang Yinliang , Du Chunyuan , Wang Wei , Qiao Wei , Li Yuhui , Zhang Yujie , Sheng Sufang , Zhou Xuenan , Zhang Lei , Fan Heng , Yu Ying , Chen Yong , Liao Yunfei , Chen Shihong , Chang Yongsheng
Published: February 2024

The mechanisms underlying glucocorticoid (GC)-induced obesity are poorly understood. Macrophages are the primary targets by which GCs exert pharmacological effects and perform critical functions in adipose tissue homeostasis. Here, we show that macrophages are essential for GC-induced obesity. Dexamethasone (Dex) strongly induced Krüppel-like factor 9 (Klf9) expression in macrophages. Similar to Dex, lentivirus-mediated Klf9 overexpression inhibits M1 and M2a markers expression, causing macrophage deactivation. Furthermore, the myeloid-specific Klf9 transgene promotes obesity. Conversely, myeloid-specific Klf9-knockout (mKlf9KO) mice are lean. Moreover, myeloid Klf9 knockout largely blocks obesity induced by chronic GC treatment. Mechanistically, GC-inducible KLF9 recruits the SIN3A/HDAC complex to the promoter regions of Il6, Ptgs2, Il10, Arg1, and Chil3 to inhibit their expression, subsequently reducing thermogenesis and increasing lipid accumulation by inhibiting STAT3 signaling in adipocytes. Thus, KLF9 in macrophages integrates the beneficial anti-inflammatory and adverse metabolic effects of GCs and represents a potential target for therapeutic interventions.
The acidic intrinsically disordered region of the inflammatory mediator HMGB1 mediates fuzzy interactions with CXCL12
Authors: Mantonico Malisa Vittoria , De Leo Federica , Quilici Giacomo , Colley Liam Sean , De Marchis Francesco , Crippa Massimo , Mezzapelle Rosanna , Schulte Tim , Zucchelli Chiara , Pastorello Chiara , Carmeno Camilla , Caprioglio Francesca , Ricagno Stefano , Giachin Gabriele , Ghitti Michela , Bianchi Marco Emilio , Musco Giovanna
Published: February 2024

Chemokine heterodimers activate or dampen their cognate receptors during inflammation. The CXCL12 chemokine forms with the fully reduced (fr) alarmin HMGB1 a physiologically relevant heterocomplex (frHMGB1•CXCL12) that synergically promotes the inflammatory response elicited by the G-protein coupled receptor CXCR4. The molecular details of complex formation were still elusive. Here we show by an integrated structural approach that frHMGB1•CXCL12 is a fuzzy heterocomplex. Unlike previous assumptions, frHMGB1 and CXCL12 form a dynamic equimolar assembly, with structured and unstructured frHMGB1 regions recognizing the CXCL12 dimerization surface. We uncover an unexpected role of the acidic intrinsically disordered region (IDR) of HMGB1 in heterocomplex formation and its binding to CXCR4 on the cell surface. Our work shows that the interaction of frHMGB1 with CXCL12 diverges from the classical rigid heterophilic chemokines dimerization. Simultaneous interference with multiple interactions within frHMGB1•CXCL12 might offer pharmacological strategies against inflammatory conditions.
Chelation enables selectivity control in enantioconvergent Suzuki–Miyaura cross-couplings on acyclic allylic systems
Authors: Stojalnikova Violeta , Webster Stephen J. , Liu Ke , Fletcher Stephen P.
Published: February 2024

Asymmetric Suzuki–Miyaura cross-couplings with aryl boronic acids and allylic electrophiles are a powerful method to convert racemic mixtures into enantioenriched products. Currently, enantioconvergent allylic arylations are limited to substrates that are symmetrical about the allylic unit, and the absence of strategies to control regio-, E/Z- and enantioselectivity in acyclic allylic systems is a major restriction. Here, using a system capable of either conjugate addition or allylic arylation, we have discovered the structural features and experimental conditions that allow an acyclic system to undergo chemo- and regioselective, enantioconvergent allylic Suzuki–Miyaura-type arylation. A wide variety of boronic acid coupling partners can be used, and both alkyl and aromatic substituents are tolerated on the allylic unit so that a wide variety of structures can be obtained. Preliminary mechanistic studies reveal that the chelating ability of the ester group is crucial to obtaining high regio- and enantioselectivity. Using this method, we were able to synthesize the natural products (S)-curcumene and (S)-4,7-dimethyl-1-tetralone and the clinically used antidepressant sertraline (Zoloft).
Bistability between π-diradical open-shell and closed-shell states in indeno[1,2-a]fluorene
Authors: Mishra Shantanu , Vilas-Varela Manuel , Lieske Leonard-Alexander , Ortiz Ricardo , Fatayer Shadi , Rončević Igor , Albrecht Florian , Frederiksen Thomas , Peña Diego , Gross Leo
Published: February 2024

Indenofluorenes are non-benzenoid conjugated hydrocarbons that have received great interest owing to their unusual electronic structure and potential applications in nonlinear optics and photovoltaics. Here we report the generation of unsubstituted indeno[1,2-a]fluorene on various surfaces by the cleavage of two C–H bonds in 7,12-dihydroindeno[1,2-a]fluorene through voltage pulses applied by the tip of a combined scanning tunnelling microscope and atomic force microscope. On bilayer NaCl on Au(111), indeno[1,2-a]fluorene is in the neutral charge state, but it exhibits charge bistability between neutral and anionic states on the lower-workfunction surfaces of bilayer NaCl on Ag(111) and Cu(111). In the neutral state, indeno[1,2-a]fluorene exhibits one of two ground states: an open-shell π-diradical state, predicted to be a triplet by density functional and multireference many-body perturbation theory calculations, or a closed-shell state with a para-quinodimethane moiety in the as-indacene core. We observe switching between open- and closed-shell states of a single molecule by changing its adsorption site on NaCl.