bluuebunny/crossref_metadata_2025 huggingface dataset:
Jevons Paradox: Sustainable Development Goals and Energy Rebound in Complex Economic Systems
Authors: Fich Louise Ellegaard , Viola Silvia , Bentsen Niclas Scott
Published: August 2022

Jevons Paradox has fundamental implications on sustainable development and the UN Sustainable Development Goals (SDGs). The paradox states that technological improvements aiming to increase the energy efficiency risk causing a rebound effect, and an increase in demand, production, and resource exploitation. Third world countries undergoing early-stage technological development may be particularly vulnerable, but it is also relevant in complex economic systems, where policymaking on climate and energy building on insufficient knowledge and attention to rebound effects can impair the desired outcome in terms of climate change mitigation, resource use and sustainable development.
Structural Analysis of Electrochemical Hydrogen Compressor End-Plates for High-Pressure Applications
Authors: Caponetto Riccardo , Privitera Emanuela , Mirone Giuseppe , Matera Fabio
Published: August 2022

Transportation and portable applications already use hydrogen as fuel, but it is essential to use highly-efficient hydrogen storage methods to increase its usage in the future. The compressed form is the most utilized for transportation applications, but mechanical compressors have low efficiency when compressing low quantities of gas to high pressure. The most suitable device for hydrogen compression is the Electrochemical Hydrogen Compressor (EHC). It has the same structure as a Proton Exchange Membrane Fuel Cell (PEM-FC), but it works at very high-pressure ( 700 bar). The present work analyses the monopolar plate of an Electrochemical Hydrogen Compressor prone to hydrogen embrittlement. Irregular shape variations generate peaks of stress magnitude and triaxiality, further contributing to decreasing metal ductility at the local scale. The calculation of the stress field in such components is essential due to the possibility of failure due to the material embrittlement caused by hydrogen. The paper presents a conceptual design of an EHC operating at 700 bar and focuses on the shape and the mechanical stress of the end-plates to have conservative levels of the nominal stress states, which then are taken as the design parameter for providing adequate structural integrity and mechanical reliability to the component. The FEM analysis with Marc software—of MSC Software Corporation—identified the optimal end-plates configuration in circular plan view and active area. The plate, sized to have a deflection no greater than 0.1mm when the EHC works at 700 bar, should have the minimum thickness of 17 mm.
Resource Efficiency in the Construction Sector: Material Intensities of Residential Buildings—A German Case Study
Authors: Krause Karina , Hafner Annette
Published: August 2022

This article describes an approach for comparing material intensity values for residential buildings with different construction types. Based on the working drawings of the different construction types (wood and mineral), material intensities are calculated at the building level. Material intensities describe the materials used in a building in mass (tonnes (t)) in relation to the square meters (m2) of gross floor area (GFA) or the cubic meters (m3) of gross volume (GV). The method for determining material intensities at the building level is demonstrated. The results show that material intensities range from 0.61 t/m2 GFA to 1.95 t/m2 GFA for single-family residential buildings and from 1.36 t/m2 GFA to 1.54 t/m2 GFA for multi-storey residential buildings. The average material intensity for mineral buildings is twice as high as that for wood buildings, which means that there is a beneficial resource efficiency in building with wood instead of mineral materials. Therefore, benchmarks for a resource efficient building can be conducted based on these values. These values demonstrate a possibility to influence resource efficiency in buildings.
Identification of New Copy Number Variation and the Evaluation of a CNV Detection Tool for NGS Panel Data in Polish Familial Hypercholesterolemia Patients
Authors: Rutkowska Lena , Pinkier Iwona , Sałacińska Kinga , Kępczyński Łukasz , Salachna Dominik , Lewek Joanna , Banach Maciej , Matusik Paweł , Starostecka Ewa , Lewiński Andrzej , Płoski Rafał , Stawiński Piotr , Gach Agnieszka
Published: August 2022

Familial hypercholesterolemia (FH) is an inherited, autosomal dominant metabolic disorder mostly associated with disease-causing variant in LDLR, APOB or PCSK9. Although the dominant changes are small-scale missense, frameshift and splicing variants, approximately 10% of molecularly defined FH cases are due to copy number variations (CNVs). The first-line strategy is to identify possible pathogenic SNVs (single nucleotide variants) using multiple PCR, Sanger sequencing, or with more comprehensive approaches, such as NGS (next-generation sequencing), WES (whole-exome sequencing) or WGS (whole-genome sequencing). The gold standard for CNV detection in genetic diagnostics are MLPA (multiplex ligation-dependent amplification) or aCGH (array-based comparative genome hybridization). However, faster and simpler analyses are needed. Therefore, it has been proposed that NGS data can be searched to analyze CNV variants. The aim of the study was to identify novel CNV changes in FH patients without detected pathogenic SNVs using targeted sequencing and evaluation of CNV calling tool (DECoN) working on gene panel NGS data; the study also assesses its suitability as a screening step in genetic diagnostics. A group of 136 adult and child patients were recruited for the present study. The inclusion criteria comprised at least “possible FH” according to the Simon Broome diagnostic criteria in children and the DLCN (Dutch Lipid Clinical Network) criteria in adults. NGS analysis revealed potentially pathogenic SNVs in 57 patients. Thirty selected patients without a positive finding from NGS were subjected to MLPA analysis; ten of these revealed possibly pathogenic CNVs. Nine patients were found to harbor exons 4–8 duplication, two harbored exons 6–8 deletion and one demonstrated exon 9–10 deletion in LDLR. To test the DECoN program, the whole study group was referred for bioinformatic analysis. The DECoN program detected duplication of exons 4–8 in the LDLR gene in two patients, whose genetic analysis was stopped after the NGS step. The integration of the two methods proved to be particularly valuable in a five-year-old girl presenting with extreme hypercholesterolemia, with both a pathogenic missense variant (c.1747C>T) and exons 9–10 deletion in LDLR. This is the first report of a heterozygous deletion of exons 9 and 10 co-occurring with SNV. Our results suggest that the NGS-based approach has the potential to identify large-scale variation in the LDLR gene and could be further applied to extend CNV screening in other FH-related genes. Nevertheless, the outcomes from the bioinformatic approach still need to be confirmed by MLPA; hence, the latter remains the reference method for assessing CNV in FH patients.
Genome-Wide Characterization of Nitrogenase Reductase (nifH) Genes in the Sweet Potato [Ipomoea batatas (L.) Lam] and Its Wild Ancestors
Authors: Si Zengzhi , Wang Chong , Zhao Mingming , Ji Zhixin , Qiao Yake , Wang Lianjun
Published: August 2022

The sweet potato (Ipomoea batatas (L.) Lam.) is an important and widely grown crop, and the nitrogenase reductase (nifH) gene is the most widely sequenced marker gene used to identify nitrogen-fixing bacteria and archaea. There have been many examples of the isolation of the diazotrophic endophytes in sweet potatoes, and there has been no report on whether sweet potatoes and their wild ancestors harbored nifH genes. In this study, a comprehensive analysis of nifH genes has been conducted on these species by using bioinformatics and molecular biology methods. A total of 20, 19 and 17 nifH genes were identified for the first time in sweet potatoes, I. trifida and I. triloba, respectively. Based on a phylogenetic analysis, all of the nifH genes, except for g10233.t1, itf14g14040.t1 and itb14g15470.t1, were clustered into five independent clades: I, II, III, IV and V. The nifH genes clustered in the same phylogenetic branch showed a more similar distribution of conserved motifs and exons–introns than those of the other ones. All of the identified genes were further mapped on the 15 chromosomes of the sweet potato, I. trifida and I. triloba. No segmental duplication was detected in each genome of three Ipomoea species, and 0, 8 and 7 tandemly duplicated gene pairs were detected in the genome of the sweet potato, I. trifida and I. triloba, respectively. Synteny analysis between the three Ipomoea species revealed that there were 7, 7 and 8 syntenic gene pairs of nifH genes detected between the sweet potato and I. trifida, between the sweet potato and I. triloba and between I. trifida and I. triloba, respectively. All of the duplicated and syntenic nifH genes were subjected to purifying selection inside duplicated genomic elements during speciation, except for the tandemly duplicated gene pair itf11g07340.t2_itf11g07340.t3, which was subjected to positive selection. Different expression profiles were detected in the sweet potato, I. trifida and I. triloba. According to the above results, four nifH genes of the sweet potato (g950, g16683, g27094 and g33987) were selected for quantitative real-time polymerase chain reaction (qRT-PCR) analysis in two sweet potato cultivars (Eshu 15 and Long 9) under nitrogen deficiency (N0) and normal (N1) conditions. All of them were upregulated in the N1 treatment and were consistent with the analysis of the RNA-seq data. We hope that these results will provide new insights into the nifH genes in the sweet potato and its wild ancestors and will contribute to the molecular breeding of sweet potatoes in the future.
Effect of Geo-Material on Dynamic Response of Tunnel Subjected to Surface Explosion
Authors: Mandal Jagriti , Goel Manmohan Dass
Published: August 2022

Prime materials involved in a problem such as underground structures are concrete, reinforcement steel, and geo-material surrounding the tunnel. Among these three materials, concrete and steel are manufactured materials and their properties can be controlled up to a certain extent. However, geo-material is a naturally occurring material whose constitutive properties vary from region to region, making it highly unpredictable. Findings from one study cannot be applied to other geotechnical problems directly, especially in the case of tunnels subjected to surface explosions. The blast wave generated has to travel through the geo-material before it interacts with the tunnel. As the shock wave propagates radially, its characteristics are likely to be altered by the geo-material. Limited study has been carried out considering this problem. In the present study, the effect of various types of geo-material on the blast response of tunnels subjected to surface explosions is investigated. Finite element analysis has been carried out using LS-DYNA®, wherein the problem has been modeled using the multi-material arbitrary Lagrangian–Eulerian (MM-ALE) method. Materials with fluid behavior such as air, explosives, and soil are modeled using ALE formulation. Other materials including tunnel lining, reinforcement steel, and rock are modeled using Lagrangian formulation. Blast loading is simulated using the Jones–Wilkins–Lee (JWL) equation of state. Geo-materials considered for the comparative study are sandy loam, saturated clayey soil, sandstone, and granite. Vertical displacement measured at the crown of the tunnel is used to determine the response of the tunnel. Sandy loam soil, being a highly compressible soil, exhibits non-linear and fluid-like behavior under high-strain loading such as explosions. Tunnels undergo extreme deformation in the case of sandy loam soil and clayey soil compared to rock cases. Further, the effect of saturation in sandy loam on tunnel stability is studied. It is observed that with the increase in saturation of soil, more blast energy is transmitted to the structure, which results in higher deformation. Lastly, the effect of the weathering of rock on the tunnel’s response is investigated in the case of sandstone and granite. It was observed that weathering in rock led to more displacement of tunnel crown when compared to intact rock.
The Clinical Significance of Urinary Retinol-Binding Protein 4: A Review
Authors: Ratajczyk Krzysztof , Konieczny Andrzej , Czekaj Adrian , Piotrów Paweł , Fiutowski Marek , Krakowska Kornelia , Kowal Paweł , Witkiewicz Wojciech , Marek-Bukowiec Karolina
Published: August 2022

Effective biomarkers for early diagnosis, prognostication, and monitoring in renal diseases (in general) comprise an unmet need. Urinary retinol-binding protein 4, which is the most sensitive indicator of renal tubular damage, holds great promise as a universal biomarker for renal pathologies, in which tubular injury is the driving force. Here, we summarize the most important existing data on the associations between urinary retinol-binding protein 4 and renal diseases and highlight the untapped potential of retinol-binding protein 4 in clinical use.
Raising the Child—Do Screen Media Help or Hinder? The Quality over Quantity Hypothesis
Authors: Puzio Diana , Makowska Iwona , Rymarczyk Krystyna
Published: August 2022

Screen media are ubiquitous in human life across all age, cultural and socioeconomic groups. The ceaseless and dynamic growth of technological possibilities has given rise to questions regarding their effect on the well-being of children. Research in this area largely consists of cross-sectional studies; experimental and randomized studies are rare, which makes drawing causative conclusions difficult. However, the prevailing approach towards the use of screen media by children has focused on time limitations. The emerging evidence supports a more nuanced perspective. It appears that the older the child, the more important how the screen media are used becomes. Concentrating on the quality of the screen, time has become increasingly relevant in the recent COVID-19 pandemic, which necessitated a transfer of educational and social functioning from real-life to the digital world. With this review, we aimed at gathering current knowledge on the correlations of different screen media use and development outcomes, as well as providing an overview of potential benefits that new technologies may provide to the pediatric population. To summarize, if one cannot evade screen time in children, how can we use it for children’s maximum advantage?
Postoperative Lifestyle of Patients with Liver Cancer: An Exploratory Study in a Single Center in Taiwan
Authors: Yan Sheng-Lei , Hu Chia-Jung , Tsai Li-Yun , Hsu Chen-Yuan
Published: August 2022

Various treatments are available for patients with liver cancer; however, complications after treatment affect their quality of life (QOL). To improve the QOL of patients with liver cancer, this study investigated the postoperative lifestyle of sixty patients at the Liouying District Hospital, Taiwan. A self-reported structured questionnaire and a modified Chinese version of the Health Enhancement Lifestyle Profile (HELP-C) were used to collect the demographic data and to assess patients’ overall postoperative lifestyle, respectively. Significant differences were observed between the overall postoperative lifestyle and the demographic variables of age, ethnicity, education level, marital status, chronic diseases, and postoperative complications (p < 0.05). Significant differences (p < 0.05) were observed in the HELP-C domains of diet, leisure, and activities of daily living (ADL) between the sexes. The scores for diet (9.66 ± 4.21) and leisure (4.33 ± 2.03) in women were significantly lower (p < 0.05) than those in men (13.13 ± 4.98 and 6.17 ± 2.37, respectively), indicating that women with liver cancer should be more concerned about diet and leisure after surgery. However, the score for ADL was significantly higher (p < 0.05) in women (17.90 ± 5.15) than in men (13.48 ± 2.56), indicating that men should focus on improving ADL. This research provides reference clinical data on the postoperative lifestyle of patients with liver cancer to improve their QOL.
Before and Amid COVID-19 Pandemic, Self-Perception of Digital Skills in Saudi Arabia Higher Education: A Longitudinal Study
Authors: Salem Mostafa Aboulnour , Alsyed Wafaa Hassanien , Elshaer Ibrahim A.
Published: August 2022

Compatible with global sustainable development report, 2016 edition, and vision 2030, Saudi Arabia recognized the importance of technology in achieving the Sustainable Development Goals (SDGs). This paper aims to measure the self-perception of digital skills among students in Saudi Arabia’s higher education system to understand how they were influenced before and amid the COVID-19 pandemic. In 2019 before the COVID-19 pandemic, we started a project to study the self-perception of digital skills among Saudi Arabia university students (group A). A total of 469 students participated in this research. The validity and reliability of the employed scale were tested with first-order confirmatory factor analysis (CFA). The differences between the two groups (before and amid the pandemic) were tested through the Mann–Whitney U test. The results for group A (N = 232 students) showed a higher self-perception of their digital skills. In March 2020, amid the pandemic, Saudi Arabia closed and shifted to technology-based teaching like many other countries worldwide. After students’ return to universities in 2021, an evaluation of how the students perceived their own digital skills was again conducted (group B). The results for group B (N = 237 students) demonstrated a lower level of confidence in their own digital abilities. Comparing two groups (A and B), after the educational course was administered, group A (prior to COVID-19) had greater self-perceptions of digital skills than group B (amid COVID-19). Students’ perceptions of their own digital skills have been negatively impacted as a result of the pandemic situation caused by COVID-19. The collected evidence suggests that there is a difference, and that this difference is statistically significant. As a result of the substantial relationship between self-perception of digital skills and how students deal with reality based on their own self-perception, Saudi Arabia higher education ministry shifted teaching methods to be based on technology. Other significant findings and their implications for practice and theory were reported in this study. Finally, limitations and prospects for future research were also elaborated.
Modeling Sleep Quality Depending on Objective Actigraphic Indicators Based on Machine Learning Methods
Authors: Bitkina Olga Vl. , Park Jaehyun , Kim Jungyoon
Published: August 2022

According to data from the World Health Organization and medical research centers, the frequency and severity of various sleep disorders, including insomnia, are increasing steadily. This dynamic is associated with increased daily stress, anxiety, and depressive disorders. Poor sleep quality affects people’s productivity and activity and their perception of quality of life in general. Therefore, predicting and classifying sleep quality is vital to improving the quality and duration of human life. This study offers a model for assessing sleep quality based on the indications of an actigraph, which was used by 22 participants in the experiment for 24 h. Objective indicators of the actigraph include the amount of time spent in bed, sleep duration, number of awakenings, and duration of awakenings. The resulting classification model was evaluated using several machine learning methods and showed a satisfactory accuracy of approximately 80–86%. The results of this study can be used to treat sleep disorders, develop and design new systems to assess and track sleep quality, and improve existing electronic devices and sensors.
Sensory Processing Sensitivity and Gastrointestinal Symptoms in Japanese Adults
Authors: Iimura Shuhei , Takasugi Satoshi
Published: August 2022

Background: Sensory processing sensitivity is a personality or temperamental trait defined as individual differences in the tendency to perceive and process both positive and negative stimuli and experiences. Studies have shown that high sensitivity is correlated with psychosocial health, including depression and anxiety. However, its relationship with physical health has not been clarified. To fill this gap, using a large sample size with sufficient statistical power, an adult sample not including university students, and a range of covariates, this study examined the association between gastrointestinal symptoms as an indicator of physical health and sensory processing sensitivity. Methods: In this cross-sectional study, the participants were 863 Japanese adults (female = 450; male = 413; Mage = 30.4 years; SD = 4.9) who completed a web-based questionnaire. We statistically controlled for sociodemographic characteristics and examined whether sensory processing sensitivity is correlated with gastrointestinal symptoms. Results: The results showed that highly sensitive individuals were more likely to experience a wide range of gastrointestinal symptoms in the past week, including reflux symptoms, abdominal pain, indigestion symptoms, diarrhea symptoms, and constipation symptoms, even when statistically controlling for the participants’ sociodemographic characteristics. Conclusions: Our findings suggest that high sensory processing sensitivity is associated with physical health. Some of the potential causes of this are also discussed.
Temporary and Permanent Auditory Effects Associated with Occupational Coexposure to Low Levels of Noise and Solvents
Authors: Bohn Vanessa , Morata Thais C. , Roggia Simone , Zucki Fernanda , Pouyatos Benoît , Venet Thomas , Krieg Edward , José Maria Renata , de Lacerda Adriana B. M.
Published: August 2022

This study aimed to assess temporary and permanent auditory effects associated with occupational coexposure to low levels of noise and solvents. Cross-sectional study with 25 printing industry workers simultaneously exposed to low noise (<80 dBA TWA) and low levels of solvents. The control group consisted of 29 industry workers without the selected exposures. Participants answered a questionnaire and underwent auditory tests. Auditory fatigue was measured by comparing the acoustic reflex threshold before and after the workday. Workers coexposed to solvents and noise showed significantly worse results in auditory tests in comparison with the participants in the control group. Auditory brainstem response results showed differences in III–V interpeak intervals (p = 0.046 in right ear; p = 0.039 in left ear). Mean dichotic digits scores (exposed = 89.5 ± 13.33; controls = 96.40 ± 4.46) were only different in the left ear (p = 0.054). The comparison of pre and postacoustic reflex testing indicated mean differences (p = 0.032) between the exposed (4.58 ± 6.8) and controls (0 ± 4.62) groups. This study provides evidence of a possible temporary effect (hearing fatigue) at the level of the acoustic reflex of the stapedius muscle. The permanent effects were identified mainly at the level of the high brainstem and in the auditory ability of binaural integration.
Assessing the Content Validity of the Revised Health of the Nation Outcome Scales (HoNOS 2018)
Authors: Harris Meredith G. , Tapp Caley , Arnautovska Urska , Coombs Tim , Dickson Rosemary , James Mick , Painter Jon , Smith Mark , Jury Angela , Lai Jennifer , Burgess Philip M.
Published: August 2022

The Health of the Nation Outcome Scales (HoNOS) comprises 12 scales that cover the kinds of problems that may be experienced by working-age adults in contact with specialised mental health services. Drawing on 20 years’ experience in clinical practice, a collaborative, international review of the HoNOS was undertaken and a revised measure (known as the HoNOS 2018) was published. In this study, 32 experts from Australia, England and New Zealand completed an anonymous web-based survey to assess the relevance, comprehensiveness and comprehensibility (aspects of content validity) of the HoNOS 2018. The experts rated 11 of the 12 HoNOS 2018 scales as ‘important’ or ‘very important’ for determining the overall clinical severity (item-level content validity index or I-CVI ≥ 0.75). Evaluations of the scales’ ability to capture change, comprehensiveness and comprehensibility were more variable, but generally positive. Experts’ comments provided further insights into this variability; for example, they noted that some scales combine multiple phenomena, which can result in ambiguity in item wording and assessment challenges. Results from this study suggest that the revisions have not altered the importance of the scales. Given the measure’s breadth of content, training remains important for ensuring rating fidelity. Inter-rater reliability and utility testing are indicated.
Clinical Performance of a Multivariate Index Assay in Detecting Early-Stage Ovarian Cancer in Filipino Women
Authors: Velayo Clarissa L. , Reforma Kareen N. , Sicam Renee Vina G. , Diwa Michele H. , Sy Alvin Duke R. , Tantengco Ourlad Alzeus G.
Published: August 2022

This study evaluated the clinical performance and overall utility of a multivariate index assay in detecting early-stage ovarian cancer in a Filipino population. This is a prospective cohort study among Filipino women undergoing assessment for an ovarian mass in a tertiary center. Patients diagnosed with early-stage ovarian cancer and who underwent a physical examination before level III specialist ultrasonographic and Doppler evaluation, multivariate index assay (MIA2G), and surgery for an adnexal mass were included in this study. Ovarian tumors were classified as high-risk for malignancy based on the IOTA-LR2 score. The ovarian imaging and biomarker results were correlated with the reference standard: surgico-pathologic findings. The MIA2G exhibited the best overall performance among individual classifiers with a sensitivity of 91.7% and NPV of 84.7%, with a concomitant higher sensitivity in early-stage disease, whether as an individual classifier (93.5%) or in serial combination with ultrasound (85.5%). The performance of biomarkers (specificity, positive predictive values, and AUROC) such as MIA2G and CA-125 significantly improved when combined with an ultrasound risk scoring approach (p < 0.01). MIA2G showed a higher sensitivity for detecting lesions among EOC and late-stage ovarian cancers than otherwise. The application of biomarkers for evaluating ovarian masses in our local setting is secondary to ultrasound but adopting multivariate index assays rather than CA-125 would increase the detection of early-stage ovarian cancers regardless of menopausal status. This is most relevant in areas where level III sonographers or gynecologic oncologists are limited and preoperative referrals to these specialists can improve the survival of our patients.
Feasibility and Comparative Effectiveness for the Delivery of the National Diabetes Prevention Program through Cooperative Extension in Rural Communities
Authors: Gorczyca Anna M. , Washburn Richard A. , Smith Patricia , Montgomery Robert N. , Koon Lyndsie M. , Hastert Mary , Suire Kameron B. , Donnelly Joseph E.
Published: August 2022

The U.S. Cooperative Extension Service (CE) has potential to deliver the National Diabetes Prevention Program (NDPP) to rural residents with prediabetes. However, the CE remains underutilized for the delivery of NDPP. We compared the feasibility/effectiveness of the NDPP (0–6 mos.) delivered by CE personnel to rural residents with prediabetes using Zoom® (CE-Zoom®) or by our research staff using Facebook® (FB). Adults (n = 31, age ~55 years) were enrolled (CE-Zoom® n = 16, FB n = 15). Attendance did not differ significantly between groups (CE Zoom® = 69%, FB = 83%, p = 0.15). Participant retention was similar in the CE Zoom® (88%) and FB groups (87%). CE-Zoom® and FB® groups provided weekly, self-monitoring data for 83% and 84% of the 24 potential weeks, respectively. Six-month weight loss was not different between groups (CE-Zoom® = −5.99 ± 8.0 kg, −5.4%, FB = −1.68 ± 3.3 kg, −1.6% p = 0.13). Participants achieving ≥5% weight loss was greater in the CE-Zoom® (44%) compared with the FB group (7%, p = 0.04). Participants achieving the NDPP program goal for physical activity (≥150 min/week) did not differ (CE-Zoom® = 75%, FB = 67%, p = 0.91). This pilot trial demonstrated the potential feasibility and effectiveness of the NDPP delivered by CE personnel in a group remote format (Zoom®) to adults with prediabetes living in rural areas.
New Progress in Early Diagnosis of Atherosclerosis
Authors: Meng Heyu , Ruan Jianjun , Yan Zhaohan , Chen Yanqiu , Liu Jinsha , Li Xiangdong , Meng Fanbo
Published: August 2022

Coronary atherosclerosis is a potentially chronic circulatory condition that endangers human health. The biological cause underpinning cardiovascular disease is coronary atherosclerosis, and acute cardiovascular events can develop due to thrombosis, platelet aggregation, and unstable atherosclerotic plaque rupture. Coronary atherosclerosis is progressive, and three specific changes appear, with fat spots and stripes, atherosclerosis and thin-walled fiber atherosclerosis, and then complex changes in arteries. The progression and severity of cardiovascular disease are correlated with various levels of calcium accumulation in the coronary artery. The therapy and diagnosis of coronary atherosclerosis benefit from the initial assessment of the size and degree of calcification. This article will discuss the new progress in the early diagnosis of coronary atherosclerosis in terms of three aspects: imaging, gene and protein markers, and trace elements. This study intends to present the latest methods for diagnosing patients with early atherosclerosis through a literature review.
Effect of FeSi Alloy Additions and Calcium Treatment on Non-Metallic Inclusions in 304 Stainless Steel during AOD and LF Refining Process
Authors: Zhai Jun , Shi Chengbin , Lang Weiyun , Zhao Yu , Wang Shijun
Published: August 2022

Non-deformable inclusions are detrimental to the surface quality and mechanical properties of stainless-steel plates. Plant trials were conducted to investigate the effect of different ferrosilicon alloys and calcium treatment during argon oxygen decarburization (AOD) and ladle furnace (LF) refining on inclusions in Si-killed 304 stainless steel. The inclusions were examined by scanning electron microscope with energy dispersive spectrometer. The results show that both the contents of soluble aluminum in molten steel and Al2O3 in slag increase with the increase of aluminum content in FeSi alloy. The content of soluble aluminum in liquid steel could be limited to lower than 0.004% when using ultra-purity FeSi alloy. When the calcium wire addition is 2 m/t, inclusions are located in the low-melting-temperature region, and the inclusion rating of hot rolled plates is mainly C-class. Industrial application shows that, by decreasing the soluble aluminum content in liquid steel, decreasing the MgO and Al2O3 in slag in AOD, and applying low basicity refining slag as well as calcium treatment, the inclusions are low melting point silicates. The inclusion rating of hot rolled plates is mainly fine C-type with a small amount of class-A, and surface polishing qualification rate is increased from 17.8% to more than 88.7%.
Mechanism Analysis and Multi-Scale Protection Design of GaN HEMT Induced by High-Power Electromagnetic Pulse
Authors: Wang Lei , Chai Changchun , Zhao Tianlong , Li Fuxing , Qin Yingshuo , Yang Yintang
Published: August 2022

Currently, severe electromagnetic circumstances pose a serious threat to electronic systems. In this paper, the damage effects of a high-power electromagnetic pulse (EMP) on the GaN high-electron-mobility transistor (HEMT) were investigated in detail. The mechanism is presented by analyzing the variation in the internal distribution of multiple physical quantities in the device. The results reveal that the device damage was dominated by different thermal accumulation effects such as self-heating, avalanche breakdown and hot carrier emission during the action of the high-power EMP. Furthermore, a multi-scale protection design for the GaN HEMT against high-power electromagnetic interference (EMI) is presented and verified by a simulation study. The device structure optimization results demonstrate that the symmetrical structure, with the same distance from the gate to drain (Lgd) and gate to source (Lgs), possesses a higher damage threshold compared to the asymmetrical structure, and that a proper passivation layer, which enhances the breakdown characteristics, can improve the anti-EMI capability. The circuit optimization results present the influences of external components on the damage progress. The findings show that the resistive components which are in series at the source and gate will strengthen the capability of the device to withstand high-power EMP damage. All of the above conclusions are important for device reliability design using gallium nitride materials, especially when the device operates under severe electromagnetic circumstances.
Corded Ware and Contemporary Hunter-Gatherer Pottery from Southeast Lithuania: Technological Insights through Geochemical and Mineralogical Approaches
Authors: Šatavičė Eglė , Skridlaitė Gražina , Grigoravičiūtė-Puronienė Inga , Kareiva Aivaras , Selskienė Aušra , Suzdalev Sergej , Žalūdienė Gailė , Taraškevičius Ričardas
Published: August 2022

A geochemical and mineralogical approach was used to analyze 3rd millennium BCE pottery from Southeast Lithuania that is attributed to the foreign Corded Ware Culture and local hunter-gatherers. SEM-EDS, XRF, XRD, and FTIR were used to study the peculiarities of the pottery and to develop hypotheses about the raw material and technology choices present. The amounts of ten major elements in the bulk and clay matrix compositions (XRF, SEM-EDS) and eleven trace analytes in the bulk compositions (XRF) were compared with the Clarke values and tested to highlight the significance (Mann–Whitney U and Wilcoxon Matched Pairs Tests) of the differences in the elemental quantities between the clay matrix and bulk compositions, and between the lighter and darker clay matrixes. These also revealed the advantage of Ward’s clustering method using the City-block distance of bulk compositions as a tool for inter-correlating ceramics in attributing them to specific communities and locations. The XRD, FTIR, and SEM-EDS mineralogical analyses indicated a predominance of iron-rich illite clay, quartz, and alkali feldspar, in addition to very low to medium firing temperatures. All of the pottery samples consisted of hydromicaceous clay from local Quaternary glacial sediments that contain weathered granitoid fragments.
A Comparison of the Fine-Grinding Performance between Cylpebs and Ceramic Balls in the Wet Tumbling Mill
Authors: Liao Ningning , Wu Caibin , Li Jianjuan , Fang Xin , Li Yong , Zhang Zhongxiang , Yin Wenhang
Published: August 2022

This study investigated the effect of grinding media on the fine-grinding performance in the wet tumbling mill. Comparative experiments between cylpebs and ceramic balls were conducted in a laboratory wet ball mill under various conditions, such as different total masses, total surface areas, and total numbers. The results indicated that ceramic balls produce a slightly smaller instantaneous breakage rate than cylpebs due to their small bulk density at the same charge volume. However, a larger instantaneous breakage rate can be obtained by ceramic balls with the same total mass due to the larger surface area. According to experiments related to the total surface area and total number of ceramic balls, it was found that the fine grinding of ceramic balls also needs to meet a certain break force, which is mainly determined by the diameter of the ceramic balls. Moreover, two models were developed to use ceramic balls instead of cylpebs as fine-grinding media in the wet tumbling mill based on the ball size and the ratio of the total mass of cylpebs. Cylpebs can be replaced by ceramic balls with 1.16–1.75 times the diameter of cylpebs, taking up only 76%–83% of the total weight of cylpebs. When the charge volume of cylpebs is between 20% and 35% in the wet ball mill, cylpebs can be replaced by ceramic balls of the same single mass, with the total weight of the ceramic balls being only 74%–77% of that of the cylpebs.
Effects of High Hydrostatic Pressure and Storage Temperature on Fatty Acids and Non-Volatile Taste Active Compounds in Red Claw Crayfish (Cherax quadricarinatus)
Authors: Liu Chunsheng , Li Meng , Wang Yuanyuan , Yang Yi , Wang Aimin , Gu Zhifeng
Published: August 2022

The effects of high hydrostatic pressure (treated with 200, 400 and 600 MPa) and storage temperatures (4 °C and −20 °C) on the fatty acids and flavor compounds of red claw crayfish were studied. HHP decreased the PUFA, GMP, IMP and AMP, citric and lactic acids, and PO43− contents, but the FAA, Ca2+ and Cl− contents increased in HHP-treated crayfish compared to untreated crayfish at 0 d. Storage at −20 °C could restrain the fatty acids and flavor contents compared to those stored at 4 °C. The GMP, AMP, citric acid and PO43− contents decreased, and Ca2+ and Cl− contents increased after storage at 4 °C for 15 d (p < 0.05). HHP at 200 and 400 MPa increased EUC on 0 d. No significant changes in EUC were observed after storage at −20 °C for 15 d, significant decreases were noted at 4 °C than the crayfish stored for 0 d (p < 0.05), except for the untreated group. Generally, HHP at 200 or 400 MPa, and storage at −20 °C is beneficial according to the shelling rates and EUC of crayfish.
Comprehensive Evaluation of the Quality of Tripterygium Glycosides Tablets Based on Multi-Component Quantification Combined with an In Vitro Biological Assay
Authors: Wang Yadan , Dai Zhong , Yan Jiangong , Wu Xianfu , Ma Shuangcheng
Published: August 2022

Tripterygium glycosides tablets (TGTs) are widely used in clinical practice to treat rheumatoid arthritis and other autoimmune diseases, with significant beneficial effects but also high toxicity, necessitating rigorous quality evaluation and control. In current study, a rapid resolution liquid chromatography tandem electrospray ionization triple quadrupole mass spectrometry (RRLC–ESI–MS/MS) method was developed and validated for the quantitative analysis of 14 components of ten batches of TGTs produced by different manufacturers, including four diterpenoids, three triterpenoids, and seven sesquiterpene alkaloids. Meanwhile, the NO inhibition effects of these TGTs were evaluated in LPS-induced RAW264.7 cells for their downstream anti-inflammatory activities, as well as their cytotoxicity. The results indicate that the TGTs from different manufacturers showed poor quality consistency, as evidenced by large variations in chemical profiles and biological effects, which may increase the risks associated with clinical use. To improve the quality status of TGTs, it is crucial to identify indicator components whose characterization can accurately reflect the efficacy and toxicity of TGTs from which they were derived. Our study reveals that triptolide, triptoquinone B, celastrol, and demethylzelaysteral considerably contributed to the anti-inflammatory activity and/or cytotoxicity of TGTs, implying that they should be further investigated as candidate indicator components for TGT quality control.
Application of Orange Peel Waste as Adsorbent for Methylene Blue and Cd2+ Simultaneous Remediation
Authors: Giraldo Stephanie , Acelas Nancy Y. , Ocampo-Pérez Raúl , Padilla-Ortega Erika , Flórez Elizabeth , Franco Camilo A. , Cortés Farid B. , Forgionny Angélica
Published: August 2022

Pollution by dyes and heavy metals is one of the main concerns at the environmental level due to their toxicity and inefficient elimination by traditional water treatment. Orange peel (OP) without any treatment was applied to effectively eliminate methylene blue (MB) and cadmium ions (Cd2+) in mono- and multicomponent systems. Although the single adsorption processes for MB and Cd2+ have been investigated, the effects and mechanisms of interactions among multicomponent systems are still unclear. Batch experiments showed that in monocomponent systems, the maximum adsorption capacities were 0.7824 mmol g−1 for MB and 0.2884 mmol g−1 for Cd2+, while in multicomponent systems (Cd2+ and MB), both contaminants competed for the adsorption sites on OP. Particularly, a synergic effect was observed since the adsorption capacity of Cd2+ increased compared to the monocomponent system. Results of desorption and adsorbent reuse confirmed that the adsorbent presents good regeneration performance. The low cost of this material and its capacity for the individual or simultaneous removal of Cd2+ and MB in aqueous solutions makes it a potential adsorbent for polluted water treatment processes.
Dyslipidemia Increases the Risk of Incident Hypertension in a Large Taiwanese Population Follow-Up Study
Authors: Lin Yu-Hsuan , Liu Yi-Hsueh , Wu Da-Wei , Su Ho-Ming , Chen Szu-Chia
Published: August 2022

Dyslipidemia is an important risk factor for hypertension and is strongly associated with an elevated risk of cardiovascular diseases including atherosclerosis and stroke. In this study, we investigated correlations between lipid profiles, including triglycerides, total cholesterol (Chol), high-and low-density lipoprotein cholesterol (HDL-C/LDL-C), and Chol/HDL-C, and baseline and incident hypertension. A total of 26,965 subjects with 4 years of follow-up data were enrolled from the Taiwan Biobank. In the cross-sectional cohort, associations between the prevalence of hypertension and lipid profiles were examined in all study participants (n = 26,965). In the longitudinal cohort, these associations were further assessed in the participants without baseline hypertension (n = 21,454). Multivariable analysis revealed that those in the second quartile (Q2) of triglycerides (compared to Q1; odds ratio (OR), 1.402; p < 0.001); Q3 of triglycerides (compared to Q1; OR, 1.365; p < 0.001); Q4 of triglycerides (compared to Q1; OR, 1.617; p < 0.001); Q3 of HDL-C (compared to Q1; OR, 0.886; p = 0.042); Q4 of HDL-C (compared to Q1; OR, 0.819; p = 0.002); Q2 of Chol/HDL-C (compared to Q1; OR, 1.144; p = 0.042); Q3 of Chol/HDL-C (compared to Q1; OR, 1.149; p = 0.034); and Q4 of Chol/HDL-C (compared to Q1; OR, 1.225; p = 0.002) were significantly associated with incident hypertension. In summary, high Chol/HDL-C, low HDL-C, and high triglycerides were associated with a higher risk of incident hypertension in the enrolled Taiwanese participants.
Effectiveness of a 12-Month Online Weight Reduction Program in Cohorts with Different Baseline BMI—A Prospective Cohort Study
Authors: Woźniak Jakub , Garbacz Katarzyna , Wojciechowska Olga , Wrzosek Michał , Włodarek Dariusz
Published: August 2022

The purpose of this article was to answer the question of whether people who want to reduce their body weight can achieve different results depending on their baseline BMI and whether the rate of weight loss is constant over the months of intervention. The study included 400 individuals aged 19 to 55 years with a mean BMI of 31.83 ± 4.77 (min 25.1 max 51.8). Men comprised 190 subjects and women 210 subjects. The participants were divided into three groups with the following BMI: overweight, class 1 obesity, and obesity class > 1 (class 2 and 3 combined). BMI groups were randomized by gender, the number of trainings per week, training time, intervention length, and intervention type. The online intervention consisted of a 15% energy deficit diet and training. Over the 12-month dietary intervention, overweight subjects reduced average body weight by 16.6%. The group with class 1 obesity reduced body weight by 15.7%. The group with obesity class > 1 reduced mean body weight by 15.4%. The relative weight reduction in the overweight group was significantly greater than in the other obesity groups (p = 0.007). In all groups, the rate of weight loss from month-to-month was statistically significant (p = 0.0001), ranging between 0.6 and 2.6% per month. The results indicate that overweight individuals are likely to experience a percent greater weight loss as a result of a comprehensive lifestyle intervention. Regardless of baseline BMI, the observed weight loss was consistent from month-to-month throughout the 12-month period, which may indicate that the diet, as well as, the training plan were properly tailored to the subjects’ needs and that they were highly motivated to participate in the program throughout its course. Properly conducted lifestyle intervention enables significant weight loss regardless of baseline BMI values.
Sodium Butyrate Effectiveness in Children and Adolescents with Newly Diagnosed Inflammatory Bowel Diseases—Randomized Placebo-Controlled Multicenter Trial
Authors: Pietrzak Anna , Banasiuk Marcin , Szczepanik Mariusz , Borys-Iwanicka Agnieszka , Pytrus Tomasz , Walkowiak Jarosław , Banaszkiewicz Aleksandra
Published: August 2022

Background: Butyric acid’s effectiveness has not yet been assessed in the pediatric inflammatory bowel disease (IBD) population. This study aimed to evaluate the effectiveness of oral sodium butyrate as an add-on to standard therapy in children and adolescents with newly diagnosed IBD. Methods: This was a prospective, randomized, placebo-controlled multicenter study. Patients aged 6–18 years with colonic Crohn’s disease or ulcerative colitis, who received standard therapy depending on the disease’s severity, were randomized to receive 150 mg sodium butyrate twice a day (group A) or placebo (group B). The primary outcome was the difference in disease activity and fecal calprotectin concentration between the two study groups measured at 12 weeks of the study. Results: In total, 72 patients with initially active disease completed the study, 29 patients in group A and 43 in group B. At week 12 of the study, the majority of patients achieved remission. No difference in remission rate or median disease activity was found between the two groups (p = 0.37 and 0.31, respectively). None of the patients reported adverse events. Conclusions: A 12-week supplementation with sodium butyrate, as adjunctive therapy, did not show efficacy in newly diagnosed children and adolescents with IBD.
Search for Novel Potent Inhibitors of the SARS-CoV-2 Papain-like Enzyme: A Computational Biochemistry Approach
Authors: Osorio Manuel I. , Yáñez Osvaldo , Gallardo Mauricio , Zuñiga-Bustos Matías , Mulia-Rodríguez Jorge , López-Rendón Roberto , García-Beltrán Olimpo , González-Nilo Fernando , Pérez-Donoso José M.
Published: August 2022

The rapid emergence and spread of new variants of coronavirus type 2, as well as the emergence of zoonotic viruses, highlights the need for methodologies that contribute to the search for new pharmacological treatments. In the present work, we searched for new SARS-CoV-2 papain-like protease inhibitors in the PubChem database, which has more than 100 million compounds. Based on the ligand efficacy index obtained by molecular docking, 500 compounds with higher affinity than another experimentally tested inhibitor were selected. Finally, the seven compounds with ADME parameters within the acceptable range for such a drug were selected. Next, molecular dynamics simulation studies at 200 ns, ΔG calculations using molecular mechanics with generalized Born and surface solvation, and quantum mechanical calculations were performed with the selected compounds. Using this in silico protocol, seven papain-like protease inhibitors are proposed: three compounds with similar free energy (D28, D04, and D59) and three compounds with higher binding free energy (D60, D99, and D06) than the experimentally tested inhibitor, plus one compound (D24) that could bind to the ubiquitin-binding region and reduce the effect on the host immune system. The proposed compounds could be used in in vitro assays, and the described protocol could be used for smart drug design.