bluuebunny/crossref_metadata_2025 huggingface dataset:
Causal effect of serum 25-hydroxyvitamin D levels on low back pain: A two-sample mendelian randomization study
Authors: Jiang Xiaojuan , Zhou Ruihao , He Yi , Zhu Tao , Zhang Weiyi
Published: September 2022

Background: Previous observational studies have suggested the involvement of 25-hydroxyvitamin D [25(OH)D] in chronic pain. However, whether the 25(OH)D is a novel target for management, the causality remains unclear.

Methods: A two-sample Mendelian randomization (MR) study was conducted to identify the causal association between 25(OH)D and low back pain (LBP). The primary analysis was revealing causality from serum 25(OH)D level (n = 417,580) on LBP (21,140 cases and 227,388 controls). The replicated analysis was performing MR estimates from circulating 25(OH)D concentration (n = 79,366) on LBP experienced last month (118,471 cases and 343,386 controls). Inverse variance weighted (IVW) was used as the main analysis. In addition, we used weighted median and MR-Egger to enhance the robustness. Sensitivity analysis was conducted to evaluate the robustness of MR results.

Results: IVW estimation indicated strong evidence that higher serum 25(OH)D levels exerted a protective effect on LBP (OR = 0.89, 95% CI = 0.83–0.96, p = 0.002). Similar trends were also found in replicate analysis (OR = 0.98, 95% CI = 0.96–1.00, p = 0.07). After meta-analysis combining primary and replicated analysis, the causal effect is significant (p = 0.03). Sensitivity analysis supported that the MR estimates were robust.

Conclusion: In our MR study, genetically increased serum 25(OH)D levels were associated with a reduced risk of LBP in the European population. This might have an implication for clinicians that vitamin D supplements might be effective for patients with LBP in clinical practice.
Systemic lupus erythematosus variants modulate the function of an enhancer upstream of TNFAIP3
Authors: Pasula Satish , Gopalakrishnan Jaanam , Fu Yao , Tessneer Kandice L. , Wiley Mandi M. , Pelikan Richard C. , Kelly Jennifer A. , Gaffney Patrick M.
Published: September 2022

TNFAIP3/A20 is a prominent autoimmune disease risk locus that is correlated with hypomorphic TNFAIP3 expression and exhibits complex chromatin architecture with over 30 predicted enhancers. This study aimed to functionally characterize an enhancer ∼55 kb upstream of the TNFAIP3 promoter marked by the systemic lupus erythematosus (SLE) risk haplotype index SNP, rs10499197. Allele effects of rs10499197, rs58905141, and rs9494868 were tested by EMSA and/or luciferase reporter assays in immune cell types. Co-immunoprecipitation, ChIP-qPCR, and 3C-qPCR were performed on patient-derived EBV B cells homozygous for the non-risk or SLE risk TNFAIP3 haplotype to assess haplotype-specific effects on transcription factor binding and chromatin regulation at the TNFAIP3 locus. This study found that the TNFAIP3 locus has a complex chromatin regulatory network that spans ∼1M bp from the promoter region of IL20RA to the 3′ untranslated region of TNFAIP3. Functional dissection of the enhancer demonstrated co-dependency of the RelA/p65 and CEBPB binding motifs that, together, increase IL20RA and IFNGR1 expression and decreased TNFAIP3 expression in the context of the TNFAIP3 SLE risk haplotype through dynamic long-range interactions up- and downstream. Examination of SNPs in linkage disequilibrium (D’ = 1.0) with rs10499197 identified rs9494868 as a functional SNP with risk allele-specific increase in nuclear factor binding and enhancer activation in vitro. In summary, this study demonstrates that SNPs carried on the ∼109 kb SLE risk haplotype facilitate hypermorphic IL20RA and IFNGR1 expression, while suppressing TNFAIP3 expression, adding to the mechanistic potency of this critically important locus in autoimmune disease pathology.
A retrospective single-center analysis of prenatal diagnosis and follow-up of 626 chinese patients with positive non-invasive prenatal screening results
Authors: Bu Xiufen , Zhou Shihao , Li Xu , Li Shihong , Li Hongyu , Ding Siyi , He Jun , Linpeng Siyuan
Published: September 2022

This study explored the diagnostic efficiency of different prenatal diagnostic approaches for women with positive non-invasive prenatal screening (NIPS) results by analyzing their clinical information and pregnancy outcomes. We collected data on 626 NIPS-positive pregnant women from January 2017 to June 2021 and arranged subsequent prenatal diagnostic operations for them after genetic counseling, along with long-term intensive follow-up. A total of 567 women accepted invasive prenatal diagnosis (IPD) (90.58%), and 262 cases were confirmed as true positives for NIPS. The positive predictive values for trisomies 21 (T21), 18 (T18), and 13 (T13); sex chromosome aneuploidies (SCAs); rare autosomal trisomies (RATs); and microdeletion and microduplication syndromes (MMS) were 81.13%, 37.93%, 18.42%, 48.83%, 18.37%, and 41.67%, respectively. Discordant results between NIPS and IPD were observed in 48 cases, with the discordance rate being 8.47%. Additionally, there were 43 cases with discordant results between karyotyping and chromosomal microarray analysis (CMA)/copy number variation sequencing. Additional reporting of RATs and MMS with routine NIPS that only detects T21/T18/T13 and SCAs can yield more accurate diagnoses. However, NIPS cannot be used as a substitute for IPD owing to its high false positive rate and discordance with other diagnostic methods. Therefore, we recommend CMA combined with karyotyping as the preferred method for accurately diagnosing NIPS-positive women.
Identification and analysis of microRNA editing events in recurrent bladder cancer based on RNA sequencing: MicroRNA editing level is a potential novel biomarker
Authors: Qin Jia-Xin , Liu Xing , Wang Xin-Lei , Wang Guang-Yue , Liang Qing , Dong Yang , Pang Kun , Hao Lin , Xue Liang , Zhao Yan , Hu Zheng-Xiang , Li Rui , Lv Qian , Chao Liu , Meng Fan-Lai , Shi Zhen-Duo , Han Cong-Hui
Published: September 2022

Background: With the continued advancement of RNA-seq (RNA-sequencing), microRNA (miRNA) editing events have been demonstrated to play an important role in different malignancies. However, there is yet no description of the miRNA editing events in recurrent bladder cancer.

Objective: To identify and compare miRNA editing events in primary and recurrent bladder cancer, as well as to investigate the potential molecular mechanism and its impact on patient prognosis.

Methods: We examined the mRNA and miRNA transcriptomes of 12 recurrent bladder cancer cases and 13 primary bladder cancer cases. The differentially expressed mRNA sequences were analyzed. Furthermore, we identified the differentially expressed genes (DEGs) in recurrent bladder cancer. The Gene Ontology (GO) functional enrichment analyses on DEGs and gene set enrichment analysis were performed. The consensus molecular subtype (CMS) classification of bladder cancer was identified using the Consensus MIBC package in R (4.1.0); miRNA sequences were then further subjected to differentially expressed analysis and pathway enrichment analysis. MiRNA editing events were identified using miRge3.0. miRDB and TargetScanHuman were used to predict the downstream targets of specific differentially edited or expressed miRNAs. The expression levels of miR-154-5p and ADAR were validated by RT-qPCR. Finally, survival and co-expression studies were performed on the TCGA-BLCA cohort.

Results: First, the mRNA expression levels in recurrent bladder cancer changed significantly, supporting progression via related molecular signal pathways. Second, significantly altered miRNAs in recurrent bladder cancer were identified, with miR-154-5p showing the highest level of editing in recurrent bladder cancer and may up-regulate the expression levels of downstream targets HS3ST3A1, AQP9, MYLK, and RAB23. The survival analysis results of TCGA data revealed that highly expressed HS3ST3A1 and RAB23 exhibited poor prognosis. In addition, miR-154 editing events were found to be significant to CMS classification.

Conclusion: MiRNA editing in recurrent bladder cancer was detected and linked with poor patient prognosis, providing a reference for further uncovering the intricate molecular mechanism in recurrent bladder cancer. Therefore, inhibiting A-to-I editing of miRNA may be a viable target for bladder cancer treatment, allowing current treatment choices to be expanded and individualized.
tRNA derived small RNAs—Small players with big roles
Authors: George Suja , Rafi Mohammed , Aldarmaki Maitha , ElSiddig Mohamed , Al Nuaimi Mariam , Amiri Khaled M. A.
Published: September 2022

In the past 2 decades, small non-coding RNAs derived from tRNA (tsRNAs or tRNA derived fragments; tRFs) have emerged as new powerful players in the field of small RNA mediated regulation of gene expression, translation, and epigenetic control. tRFs have been identified from evolutionarily divergent organisms from Archaea, the higher plants, to humans. Recent studies have confirmed their roles in cancers and other metabolic disorders in humans and experimental models. They have been implicated in biotic and abiotic stress responses in plants as well. In this review, we summarize the current knowledge on tRFs including types of tRFs, their biogenesis, and mechanisms of action. The review also highlights recent studies involving differential expression profiling of tRFs and elucidation of specific functions of individual tRFs from various species. We also discuss potential considerations while designing experiments involving tRFs identification and characterization and list the available bioinformatics tools for this purpose.
Application of phase field model coupled with convective effects in binary alloy directional solidification and roll casting processes
Authors: Zeng Hong Bo , Ai Xin Gang , Chen Ming , Hu Xiao Dong
Published: September 2022

Based on the Kim-Kim-Suzuki (KKS) phase field model coupled with the thermodynamic parameters, the transformation process from columnar dendrites to equiaxed crystals during directional solidification of aluminium alloy was simulated, and the effects of phase field parameters on the growth morphology and dendrite segregation were discussed. Furthermore, considering the effect of the microcosmic flow field, the convection influence gradient term is introduced into KKS formula near the solid-liquid interface, and the phase field model considering flow field was applied to the inherent convective environment of the actual roll casting process, also the multiple dendrites growth behavior of magnesium alloy under the action of microscopic convection was further explored. When coupling calculation of microscopic velocity field and pressure field, the staggered grid method was used to deal with the complex interface. The combined solution of Marker in Cell (MAC) algorithm and phase field discrete calculation was realized. In order to further describe the influence of convection on the solidification process, the roll casting experiments are used to verify the impact growth of multiple dendrites under convection. The results show that the dendrites undergo solute remelting and the dendrites melt into equiaxed crystals, showing the phenomenon of Columnar to Equiaxed Transition (CET).
Guanine inhibits the growth of human glioma and melanoma cell lines by interacting with GPR23
Authors: Garozzo Roberta , Zuccarini Mariachiara , Giuliani Patricia , Di Liberto Valentina , Mudò Giuseppa , Caciagli Francesco , Ciccarelli Renata , Ciruela Francisco , Di Iorio Patrizia , Condorelli Daniele F.
Published: September 2022

Guanine-based purines (GBPs) exert numerous biological effects at the central nervous system through putative membrane receptors, the existence of which is still elusive. To shed light on this question, we screened orphan and poorly characterized G protein-coupled receptors (GPRs), selecting those that showed a high purinoreceptor similarity and were expressed in glioma cells, where GBPs exerted a powerful antiproliferative effect. Of the GPRs chosen, only the silencing of GPR23, also known as lysophosphatidic acid (LPA) 4 receptor, counteracted GBP-induced growth inhibition in U87 cells. Guanine (GUA) was the most potent compound behind the GPR23-mediated effect, acting as the endpoint effector of GBP antiproliferative effects. Accordingly, cells stably expressing GPR23 showed increased sensitivity to GUA. Furthermore, while GPR23 expression was low in a hypoxanthine-guanine phosphoribosyl-transferase (HGPRT)-mutated melanoma cell line showing poor sensitivity to GBPs, and in HGPRT-silenced glioma cells, GPR23-induced expression in both cell types rescued GUA-mediated cell growth inhibition. Finally, binding experiments using [3H]-GUA and U87 cell membranes revealed the existence of a selective GUA binding (KD = 29.44 ± 4.07 nM; Bmax 1.007 ± 0.035 pmol/mg prot) likely to GPR23. Overall, these data suggest GPR23 involvement in modulating responses to GUA in tumor cell lines, although further research needs to verify whether this receptor mediates other GUA effects.
Low-dose norepinephrine in combination with hypotensive resuscitation may prolong the golden window for uncontrolled hemorrhagic shock in rats
Authors: Zhou Yuanqun , Li Qinghui , Xiang Xinming , Wu Yue , Zhu Yu , Peng Xiaoyong , Liu Liangming , Li Tao
Published: September 2022

Hypotension resuscitation is an important principle for the treatment after trauma. Current hypotensive resuscitation strategies cannot obtain an ideal outcome for remote regions. With the uncontrolled hemorrhagic shock (UHS) model in rats, the effects of norepinephrine (NE) on the tolerance time of hypotensive resuscitation, blood loss, vital organ functions, and animal survival were observed. Before bleeding was controlled, only the LR infusion could effectively maintain the MAP to 50–60 mmHg for 1 h, while the MAP gradually decreased with prolonging time, even with increasing infusion volume. Low-dose NE during hypotensive resuscitation prolonged the hypotensive tolerance time to 2–3 h, and the effect of 0.3 μg/kg/min NE was the best. Further studies showed that 0.3 μg/kg/min NE during hypotensive resuscitation significantly lightened the damage of organ function induced by UHS via protecting mitochondrial function, while the LR infusion did not. At the same time, NE administration improved Hb content, DO2, and VO2, and restored liver and kidney blood flow. The survival results showed that low-dose NE administration increased the survival rate and prolonged the survival time. Together, low-dose NE during hypotensive resuscitation was suitable for the early treatment of UHS, which can strive for the golden window of emergency treatment for serious trauma patients by reducing blood loss and protecting vital organ functions.
A subspace projection approach to quantify respiratory variations in the f-wave frequency trend
Authors: Abdollahpur Mostafa , Engström Gunnar , Platonov Pyotr G. , Sandberg Frida
Published: September 2022

Background: The autonomic nervous system (ANS) is known as a potent modulator of the initiation and perpetuation of atrial fibrillation (AF), hence information about ANS activity during AF may improve treatment strategy. Respiratory induced ANS variation in the f-waves of the ECG may provide such information.

Objective: This paper proposes a novel approach for improved estimation of such respiratory induced variations and investigates the impact of deep breathing on the f-wave frequency in AF patients.

Methods: A harmonic model is fitted to the f-wave signal to estimate a high-resolution f-wave frequency trend, and an orthogonal subspace projection approach is employed to quantify variations in the frequency trend that are linearly related to respiration using an ECG-derived respiration signal. The performance of the proposed approach is evaluated and compared to that of a previously proposed bandpass filtering approach using simulated f-wave signals. Further, the proposed approach is applied to analyze ECG data recorded for 5 min during baseline and 1 min deep breathing from 28 AF patients from the Swedish cardiopulmonary bioimage study (SCAPIS).

Results: The simulation results show that the estimates of respiratory variations obtained using the proposed approach are more accurate than estimates obtained using the previous approach. Results from the analysis of SCAPIS data show no significant differences between baseline and deep breathing in heart rate (75.5 ± 22.9 vs. 74 ± 22.3) bpm, atrial fibrillation rate (6.93 ± 1.18 vs. 6.94 ± 0.66) Hz and respiratory f-wave frequency variations (0.130 ± 0.042 vs. 0.130 ± 0.034) Hz. However, individual variations are large with changes in heart rate and atrial fibrillatory rate in response to deep breathing ranging from −9% to +5% and −8% to +6%, respectively and there is a weak correlation between changes in heart rate and changes in atrial fibrillatory rate (r = 0.38, p < 0.03).

Conclusion: Respiratory induced f-wave frequency variations were observed at baseline and during deep breathing. No significant changes in the magnitude of these variations in response to deep breathing was observed in the present study population.
Estradiol and zinc-doped nano hydroxyapatite as therapeutic agents in the prevention of osteoporosis; oxidative stress status, inflammation, bone turnover, bone mineral density, and histological alterations in ovariectomized rats
Authors: Elghareeb Mona M. , Elshopakey Gehad E. , Elkhooly Tarek A. , Salama Basma , Samy Alaa , Bazer Fuller W , Elmetwally Mohammed A , Almutairi Mikhlid H. , Aleya Lotfi , Abdel-Daim Mohamed M. , Rezk Shaymaa
Published: September 2022

Osteoporosis (OP) is a serious health problem, and the most popular therapeutic strategy for OP is hormone replacement (estrogen); however, it increases the risk of reproductive cancers. Hydroxyapatite (HA) nanoparticles have a similar chemical structure to the bone mineral component and can be used as a new remedy for OP. This study was designed to investigate the osteoporosis-protective potential of nano zinc hydroxyapatite (ZnHA-NPs) and/or estradiol (E2) combined therapy. A total of 35 adult female rats were assigned into five groups (n = 7): 1) control group; 2) ovariectomized group (OVX); 3) OVX received oral estradiol replacement therapy (OVX/E2); 4) OVX received ZnHA replacement therapy (OVX/ZnHA); and 5) OVX received both estradiol and ZnHA-NPs combined therapy (OVX/E2+ZnHA). After 3 months of treatment, serum bone markers and estrogen level, oxidative/antioxidant, and inflammatory cytokines were determined. Additionally, femoral expression of estrogen receptors alpha and beta (ESR1; ESR2), receptor activator of nuclear factor-kappa B (RANKL) ligand, osteoprotegerin (OPG), bone mineral density (BMD), histological alterations, and immunohistochemical expression of vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) were assessed. ALP, PINP, Ca, and P concentrations improved significantly (p < 0.05) in all treatment groups, especially in the OVX/E + ZnHA group. MDA and NO were higher in OVX rats, while SOD activity and GSH were lower (p < 0.05). E2 alone or with ZnHA-NPs restored the estimated antioxidant molecules and cytokines toward normal levels in OVX rats (p < 0.05). On the other hand, E2 and ZnHA increased OPG and OC expression in femurs while decreasing ESR1, ESR2, and NF-kB expression (p < 0.05). The combination treatment was superior in the restoration of normal femoral histoarchitecture and both cortical and trabecular BMD (p < 0.05). Overall, the combined therapy of OVX/E2+ZnHA was more effective than the individual treatments in attenuating excessive bone turnover and preventing osteoporosis.
A Practical Interlacing-Based Coverage Path Planning Method for Fixed-Wing UAV Photogrammetry in Convex Polygon Regions
Authors: Huang Junhua , Fu Wenxing , Luo Sheng , Wang Chenxin , Zhang Bo , Bai Yu
Published: September 2022

This paper investigates the coverage path planning problem for a fixed-wing UAV in convex polygon regions with several practical task requirements in photogrammetry considered. A typical camera model pointing forward-down for photogrammetric application is developed. In addition, the coordinates of the region vertices are converted from the WGS-84 coordinate system to the local ENU coordinate system for path planning convenience. The relationship between the minimum turning radius and the camera footprint is fully studied and the span coefficient of the fixed-wing UAV is first proposed. A novel flight pattern, named as the interlaced back-and-forth pattern in this paper, is presented accordingly. The proposed algorithm is compared with a traditional back-and-forth pattern in mathematics and several important results are given. Then, a practical low-computation algorithm for waypoints generation is developed. Finally, simulation results validate the effectiveness of the proposed method.
Efficient Numerical Integration Algorithm of Probabilistic Risk Assessment for Aero-Engine Rotors Considering In-Service Inspection Uncertainties
Authors: Li Guo , Liu Junbo , Zhou Huimin , Zuo Liangliang , Ding Shuiting
Published: September 2022

Numerical integration methods have the characteristics of high efficiency and precision, making them attractive for aero-engine probabilistic risk assessment and design optimization of an inspection plan. One factor that makes the numerical integration method a suitable approach to in-service inspection uncertainties is the explicit derivation of the integration formula and integration domains. This explicit derivation ensures accurate characterization of a multivariable system’s failure risk evolution mechanism. This study develops an efficient numerical integration algorithm for probabilistic risk assessment considering in-service inspection uncertainties. The principle of probability conservation is applied to the transformation of the integration domain from the current flight cycle to the initial (N = 0) computational space. Consequently, the integration formula of failure probability is deduced, and a detailed mathematical demonstration of the proposed method is provided. An actual compressor disk is evaluated using the efficient numerical integration algorithm and the Monte Carlo simulation to validate the accuracy and efficiency of the proposed method. Results show that the time cost of the proposed algorithm is dozens of times lower than that of the Monte Carlo simulation, with a maximum relative error of 5%. Thus, the efficient numerical integration algorithm can be applied to failure analysis in the airworthiness design of commercial aero-engine components.
Human Activity Played a Key Role in Rice Stripe Disease Epidemics: From an Empirical Evaluation of over a 10-Year Period
Authors: Ma Yan-Li , Lin Wen-Wu , Guo Si-Si , Xie Lian-Hui , He Dun-Chun , Cheng Zhao-Bang
Published: September 2022

Paddy is an artificial ecosystem driven by human activities, such as adjustment of cropping systems, deployment of resistant varieties and pesticides use. Inappropriate human intervention aggravated the disruption of ecosystems, which resulted in rice viral disease epidemics characterized by fulminant, migrating and intermittent outbreaks. Rice stripe disease (RSD), lasting for over 10 years from 2000, was modeled for exploring better management strategies of plant viral disease transmitted by insect vectors. In eight counties of Jiangsu province, China, the biotic, abiotic and human factors between 2000 and 2012 were monitored to determine key factors of human activities related to RSD epidemics. RSD severity was significantly related to resistance, the interval of wheat harvest and rice sowing (WHRS) and inconsecutive interval of wheat sowing and rice harvest (WSRH). The relationship between human activities and the small brown planthopper (SBPH) showed that the resistance was more significantly associated with SBPH viruliferous rate in the preceding year than that of the current year but not correlated with SBPH density. Resistance could impact the SBPH viruliferous rate in the preceding year indirectly through transmission probability and, thereafter, the continuing disease epidemics. The insignificant interactive effects among resistance, WHRS and WSRH on disease severity meant that these three factors could be taken into consideration separately in agricultural practice according to rice chronological order. The quantitative field study conducted in Jiangsu province presented a good example of plant viral disease management, guided by which could not only avoid pointless actions but, most importantly, generate more efficient and economic returns. Therefore, in order to improve the management of RSD, it should focus on the adjustment of these human factors independently and sequentially in combination with the forecast of RSD.
Sensitivity and Recovery of Tomato Cultivars Following Simulated Drift of Dicamba or 2,4-D
Authors: Warmund Michele R. , Ellersieck Mark R. , Smeda Reid J.
Published: September 2022

Tomato (Solanum lycopersicum L.) plants are commonly injured by the off-target movement of synthetic auxin herbicides. A greenhouse and a field trial were conducted to determine the relative tolerance of eight fresh market tomato cultivars to drift-simulating rates of dicamba or 2,4-D. Tomato cultivars included ‘BHN 589’, ‘Celebrity’, ‘Florida 91’, ‘Mountain Merit’, ‘Primo Red’, ‘Red Deuce’, ‘Red Morning’, and ‘Skyway’. Dicamba (3,6-dichloro-methoxybenzoic acid) and 2,4-D (2,4-dichlorophenoxyacetic acid) were applied at 2.8 g ae ha−1 and 5.3 g ae ha−1-D, respectively. By 14 weeks after treatment (WAT), herbicide-treated plants of each cultivar produced less total and marketable yield than their respective nontreated control in the greenhouse trial. For most cultivars, dicamba-treated plants had less marketable yield than 2,4-D-treated plants in the greenhouse. Herbicide treatments also reduced total and marketable yields of each cultivar when compared with their control in the field study at 14 WAT, except for ‘Mountain Merit’. The severity of yield loss from herbicide treatments was cultivar-dependent. Field-grown ‘Skyway’ plants treated with dicamba produced the lowest marketable yield. In contrast, herbicide-treated plants of ‘Florida 91’ produced high marketable yields in the field, but ‘Red Deuce’ plants receiving 2,4-D were also highly productive. Herbicide residue in fruit sampled the third week of the harvest was nondetectable. Because the type of auxin herbicide drift is often unanticipated, ‘Florida 91’ may be the preferred cultivar for cultivation among those tested to maximize tomato production in the field.
Relationship of Textures from Tomato Fruit Images Acquired Using a Digital Camera and Lycopene Content Determined by High-Performance Liquid Chromatography
Authors: Ropelewska Ewa , Szwejda-Grzybowska Justyna
Published: September 2022

This study aimed at correlating image features with the lycopene content of tomato fruit. Tomato cultivars with different fruit colors, such as ‘Ożarowski’ (yellow), ‘Marvel Striped’ (yellow-orange-pink), ‘Green Zebra’ (green), Sandoline F1 (red), Cupidissimo F1 (red), and Sacher F1 (brown) were selected for the study. The tomato fruits were imaged using a digital camera. The texture parameters were computed from the images converted to color channels R, G, B, L, a, b, X, Y, and Z based on the histogram, autoregressive model, gradient map, co-occurrence matrix, and run-length matrix. Lycopene content was determined using high-performance liquid chromatography (HPLC). Pearson’s correlation coefficients (R), regression equations, and coefficients of determination (R2) were determined. The lycopene content in fruit ranged from 0.31 mg 100 g−1 for ‘Green Zebra’ to 11.83 mg 100 g−1 for Sacher F1. The correlation coefficient (R) between lycopene content and selected image textures reached −0.99 for selected textures from color channels G, b, and Y. The highest positive correlation (R parameter equal to 0.98) was obtained for texture from color channel Y. Based on the individual color channel providing the highest results, one texture was selected for the determination of regression equations. Coefficients of determination (R2) of 0.99 were obtained for texture from color channel G. The regression equations may be used in practice for nondestructive, objective, and precise estimation of the lycopene content in tomato fruit.
Effect of Rearing Systems on Growth Performance, Lying/Standing Behavior, Morbidity, and Immunity Parameters of Pre-Weaned Dairy Calves in a Continental Zone in Winter
Authors: Zhao Wanying , Choi Christopher , Ru Lin , Shi Zhengxiang , Li Hao
Published: September 2022

On dairy farms, calves are typically raised inside barns (either in individual or group pens), or they are raised in outdoor hutches. To evaluate the effect of all three of these commonly used rearing practices on calves, an experiment was conducted. A group of 58 Holstein dairy female healthy calves (3 days of age) was randomly divided into three subgroups (outdoor hutches, individual-housed, and group-housed in a barn). The body weight, lying bouts, lying time, and immunity parameters of each calf were monitored, and the ambient temperature and relative humidity were measured. The average temperatures outside and in the barn and hutches were −16.67 °C, −15.26 °C, and −15.59 °C, respectively, from 22 November 2020 to 27 January 2021. All calves suffered from cold stress. Group-housed calves weighed significantly less than the other calves at the ages of 1 month and 2 month (p < 0.05). The lying time of the calves housed in individual pens and group pens was longer (p < 0.05) than that of the calves housed in hutches. The morbidity attributable to bovine respiratory disease was significantly lower among the calves housed in hutches than it was among the calves housed either individually or in group pens inside the barn (p < 0.05). No significant differences in the concentrations of TNF-α, IL-1β, and IL-6 were found between the three groups (p > 0.05). On the basis of these findings, we were able to conclude that calves housed in outdoor hutches were at a lower risk of developing a disease than were calves housed in barns without heating in winter. To optimize the management process, heating should be added to hutch systems. Moreover, more rigorous disease and environmental control management strategies should be applied when raising calves inside barns.
Responses of Wheat Yield under Different Fertilization Treatments to Climate Change Based on a 35-Year In Situ Experiment
Authors: Zhang Rui , Yang Yingnan , Dang Tinghui , Zhu Yuanjun , Huang Mingbin
Published: September 2022

Fertilization, as one of many important field management practices, can increase crop yields. However, whether different levels of fertilization will affect the response of wheat yields to inter-annual climate variations and long-term climate trends is not clear. In this study, 35-year wheat yields were used to investigate the responses of wheat yield to inter-annual climate variations and long-term climate trends under different fertilization treatments. The first difference method was used to de-trend wheat yields and climate variables and stepwise regression analysis was used to quantify the yield–climate relationship. The experimental design consisted of a control treatment (CK without fertilization) and three fertilizer treatments: nitrogen, phosphorus, and manure (NPM with 120 kg ha−1 N, 26.2 kg ha−1 P, and 75 t ha−1 manure), nitrogen and phosphorus (NP with 120 kg ha−1 N and 26.2 kg ha−1 P), and manure (M with 75 t ha−1 manure). Compared to the CK treatment, the NPM, NP, and M treatments increased wheat yield by an average of 201.9, 161.7, and 130.6% and increased yield inter-annual variability by an average of 191.2, 149.3, and 144.2%, respectively, during the study period (1985–2020). Inter-annual climate fluctuations in the study area explained 45, 38, 27, and 29% of wheat yield variations and 35-year climatic trends contributed to wheat yield decreases of 0.3, 0.7, 1.6, and 1.8% for the NPM, NP, M, and CK treatments, respectively. The results show the impact of inter-annual climate fluctuations on yield increases with the increasing level of fertilization, while the effect of long-term climate trends on yield decreases with the increasing level of fertilization.
Impact of High Temperature on Germination, Seedling Growth and Enzymatic Activity of Wheat
Authors: Sharma Sushma , Singh Vikram , Tanwar Hemender , Mor Virender Singh , Kumar Mukesh , Punia Ramesh Chander , Dalal Mohinder Singh , Khan Mujahid , Sangwan Sonali , Bhuker Axay , Dagar Chander Shekhar , Yashveer Shikha , Singh Jogender
Published: September 2022

Global warming has increased the temperature significantly over a large extent both spatially and temporally. The threat of heat stress during the germination and seedling establishment stages in the wheat crop is now more prevalent than ever before. The present experiment assessed the effect of elevated temperature on the germination and early seedling growth of wheat genotypes. The seeds were sown under four temperatures, viz., 20 °C, 25 °C, 30 °C and 35 °C; the germination, seedling vigor and enzyme activities in 8-day-old seedlings were assessed. The temperature significantly influenced germination and early seedling growth. The germination percentage at 20 and 25 °C was statistically on par with and higher than at 30 and 35 °C. The seedling vigor parameters were maximum at 25 °C and showed a reduction at higher temperatures. Genotypic differences were observed for early heat stress as the genotypes WH 730, WH 1123 and HD 2967 showed tolerance towards heat stress during germination, whereas the genotypes PBW 725 and WH 1105 were susceptible. Antioxidant enzyme activities in seedlings increased with the rise in temperature. Catalase, peroxidase and superoxide dismutase enzymes showed increased activities at higher temperature levels.
Determining Irrigation Requirements of Extensive Crops Using the Typical Meteorological Year Adjusted to the Growing Cycle Period
Authors: Cano Antonio , Pardo José Jesús , Montero Jesús , Domínguez Alfonso
Published: September 2022

In the Castilla-La Mancha (CLM, Spain) region, most of the irrigated area is managed by two different strategies in which the previously defined irrigation requirements of crops affect both the distribution of crops and the sustainable management of groundwater resources. Thus, in the western Mancha system, the amount of irrigation water per farm is limited, while in the eastern Mancha system the irrigable area per farm is limited. Therefore, the use of average irrigation requirements in these areas may cause yield drops in dry years and the overuse of groundwater. Consequently, the main aim was to achieve a better approach to the irrigation requirements of the main extensive crops in CLM (maize (Zea mays L.), onion (Allium cepa L.), garlic (Allium sativum L.), and barley (Hordeum vulgare L.)) to help farmers and water authorities achieve higher yields and a more sustainable use of water resources. The typical meteorological year (TMY) methodology combined with the MOPECO model were used to: (1) determine the distribution of wet, intermediate, and dry years during the growing cycle of the four selected crops; (2) determine the average (AVE) and typical irrigation requirements of these crops for the complete 70 years series (TMYG) or the duration of the crop cycle (TMYC), and under wet (TMYW), intermediate (TMYI), and dry (TMYD) year conditions; and (3) recommend the irrigation depths to be used for the management of farms and water bodies. The results show that the number of wet, intermediate, and dry years depends on the growing cycle of the crop considered, with wet years being unusual, although they notably increase the average rainfall in the area. The irrigation requirements for the average year were between 20.4 and 9.0% lower than the average irrigation requirements calculated for the four studied crops during the 70 years of the series. For western Mancha farmers the recommended irrigation depth for dry years and most profitable crops (garlic and onion) is the one calculated for the driest year of the series, while for the rest of the years and crops is that estimated by the global dry TMY (TMYGD). For eastern Mancha farmers the recommended irrigation depths are also those estimated by the TMYGD.
Monitoring Growth Status of Winter Oilseed Rape by NDVI and NDYI Derived from UAV-Based Red–Green–Blue Imagery
Authors: Zamani-Noor Nazanin , Feistkorn Dominik
Published: September 2022

The current study aimed to evaluate the potential of the normalized difference vegetation index (NDVI), and the normalized difference yellowness index (NDYI) derived from red–green–blue (RGB) imaging to monitor the growth status of winter oilseed rape from seeding to the ripening stage. Subsequently, collected values were used to evaluate their correlations with the yield of oilseed rape. Field trials with three seed densities and three nitrogen rates were conducted for two years in Salzdahlum, Germany. The images were rapidly taken by an unmanned aerial vehicle carrying a Micasense Altum multi-spectral camera at 25 m altitudes. The NDVI and NDYI values for each plot were calculated from the reflectance at RGB and near-infrared (NIR) bands’ wavelengths pictured in a reconstructed and segmented ortho-mosaic. The findings support the potential of phenotyping data derived from NDVI and NDYI time series for precise oilseed rape phenological monitoring with all growth stages, such as the seedling stage and crop growth before winter, the formation of side shoots and stem elongation after winter, the flowering stage, maturity, ripening, and senescence stages according to the crop calendar. However, in comparing the correlation results between NDVI and NDYI with the final yield, the NDVI values turn out to be more reliable than the NDYI for the real-time remote sensing monitoring of winter oilseed rape growth in the whole season in the study area. In contrast, the correlation between NDYI and the yield revealed that the NDYI value is more suitable for monitoring oilseed rape genotypes during flowering stages.
Predicting Plant Growth and Development Using Time-Series Images
Authors: Wang Chunying , Pan Weiting , Song Xubin , Yu Haixia , Zhu Junke , Liu Ping , Li Xiang
Published: September 2022

Early prediction of the growth and development of plants is important for the intelligent breeding process, yet accurate prediction and simulation of plant phenotypes is difficult. In this work, a prediction model of plant growth and development based on spatiotemporal long short-term memory (ST-LSTM) and memory in memory network (MIM) was proposed to predict the image sequences of future growth and development including plant organs such as ears. A novel dataset of wheat growth and development was also compiled. The performance of the prediction model of plant growth and development was evaluated by calculating structural similarity index measure (SSIM), mean square error (MSE), and peak signal to noise ratio (PSNR) between the predicted and real plant images. Moreover, the optimal number of time steps and the optimal time interval between steps were determined for the proposed model on the wheat growth and development dataset. Under the optimal setting, the SSIM values surpassed 84% for all time steps. The mean of MSE values was 46.11 and the MSE values were below 68 for all time steps. The mean of PSNR values was 30.67. When the number of prediction steps was set to eight, the prediction model had the best prediction performance on the public Panicoid Phenomap-1 dataset. The SSIM values surpassed 78% for all time steps. The mean of MSE values was 77.78 and the MSE values were below 118 for all time steps. The mean of PSNR values was 29.03. The results showed a high degree of similarity between the predicted images and the real images of plant growth and development and verified the validity, reliability, and feasibility of the proposed model. The study shows the potential to provide the plant phenotyping community with an efficient tool that can perform high-throughput phenotyping and predict future plant growth.
Identification of Phytochemical Compounds, Functional Properties and Antioxidant Activity of Germinated Purple Corn Protein Concentrate and Its Gastrointestinal Hydrolysates
Authors: Vilcacundo Edgar , Montalvo Vinicio , Sanaguano Herminia , Moran Roberto , Carrillo Wilman , García Antón
Published: September 2022

Purple corn seeds germinated at 25 °C and 35 °C for 5 days were used to obtain purple corn protein concentrate (PCPC25 and PCPC35). PCPC25 and PCPC35 were subject to gastrointestinal hydrolysis. PCPC and its hydrolysates were used to evaluate their functional and biological properties. Total flavonoid content (TFC), total flavonol content (TFLC), total anthocyanin content (TAC) and total proanthocyanin (TAP) were determined. The polyphenols and flavonoids were identified by UPLC-QDa. Protein solubility (PS), water and oil absorption capacity (WAC and OAC) were determined. The antioxidant activity was evaluated by the FRAP, ABTS and DPPH methods. PCPC35 showed the highest TFLC values (11,091.37 mg rutin equivalents (RE)/100 g dry weight DW), and TFL presented values of 7975.59 mg RE/100 g DW. The PCPCs presented better functional properties than the hydrolysates. PCPC25 presented a PS of 59.43%PS at pH 8.0, 27.77%WAC and 24.94%OAC. PCPC25 and PCPC35 showed high values of antioxidant activity. PCPC25 showed ABTS values (570.97 µmol trolox equivalents (TE)/g DW) and FRAP (772.85 µmol TE/g DW). PCPCs hydrolysates were less active with ABTS values (74.12 µmol TE/g DW) and FRAP (59.42 µmol TE/g DW).
Crop Water Requirement and Utilization Efficiency-Based Planting Structure Optimization in the Southern Huang-Huai-Hai Plain
Authors: Liu Jian , Sun Bin , Shen Hongli , Ding Pengfei , Ning Dongfeng , Zhang Jiyang , Qiu Xinqiang
Published: September 2022

Optimizing planting structure that balances both high yield and water resources shortage is essential for developing efficient water-saving agriculture. To provide insights about the relationship between planting structure optimization and water resource constraint, crop water requirement, precipitation coupling degree, gross total water requirement and irrigation project metrics were calculated and analyzed with the dataset collected from 16 locations in Xuchang City, China. The strategy of reducing the planting proportion of high water-consumption crops and increasing low consumption and high precipitation coupling degree crops was adopted to determine a suitable water-saving planting scheme based on the IQR (interquartile range) method. Evapotranspiration had a decreasing trend from northwest to southeast areas. There were positive correlations between gross total water requirement (GTWR) and annual total yields (r = 0.825, p = 0.002), and between GTWR and proportion of vegetable planting areas (PVPA) (r = 0.734, p = 0.0101). The GTWR was negatively correlated with the ratio of water-saving irrigated areas to effective irrigated areas (RSEA), proportion of wheat planting areas (PWPA) and proportion of bean planting areas (PBPA), with coefficients of −0.787, −0.936 and −0.828, respectively. The planting proportion of winter wheat, summer maize, vegetables and flowers decreased by 8.8%, 25.8%, 16.2%, and 28.7%, respectively, while oil-beans and tubers increased by 62.4% and 95.6%, respectively. The irrigation water consumption was reduced by 5.2%, saving 3.25 × 107 m3 irrigation water without sacrificing economic benefits after adjusting for the whole region. Consequently, precipitation coupling degree, water-saving technology and historical planting habits should be considered when optimizing cropping distributions. This research provided a new theoretical basis and comprehensive approach for agriculture irrigation water management and regional planting structure optimization from a realistic perspective.
Mycorrhizal Inoculation Improves the Quality and Productivity of Essential Oil Distilled from Three Aromatic and Medicinal Plants: Thymus satureioides, Thymus pallidus, and Lavandula dentata
Authors: Akachoud Oumaima , Bouamama Hafida , Facon Natacha , Laruelle Frédéric , Zoubi Btissam , Benkebboura Abderrazak , Ghoulam Cherki , Qaddoury Ahmed , Lounès-Hadj Sahraoui Anissa
Published: September 2022

In Morocco, extensive use, traditional practices, and climate change have seriously impacted the productivity of aromatic and medicinal plants (AMP). To mitigate these adverse effects, this study aims at evaluating the potential of the arbuscular mycorrhizal fungi (AMF), namely Rhizophagusirregularis and Funneliformis mosseae, in improving biomass, essential oils (EOs), and biomolecule production in Thymus satureioides, T. pallidus, and Lavandula dentata. Compared to non-inoculated-AMP, AMF induced significant increases in biomass production by 37.1, 52.4, and 43.6%, and in EOs yield by 21, 74, and 88% in T. satureioides, T. pallidus, and L. dentata, respectively. The EOs of inoculated-AMP exhibited increased proportions of major compounds such as thymol (23.7%), carvacrol (23.36%), and borneol (18.7%) in T. satureioides; α-terpinene (32.6%), thymol (28.79%), and δ-terpinene (8.1%) in T. pallidus; and camphor (58.44%), isoborneol (8.8%), and fenchol (4.1%) in L. dentata. Moreover, AMF significantly improved the anti-germinative and antifungal activities of the EOs. Indeed, IC50 values decreased by 1.8, 16.95, and 2.2 times against Blumerai graminis, Zymoseptoria tritici, and Fusarium culmorum, respectively, compared to non-inoculated-AMP. This study highlights the performance of the symbiosis between AMF and AMPs in terms of high quality of EOs production while respecting the environment. The associations F. mosseae-Thymus and R. irregularis-Lavandula are the most efficient.
Comparison of Some Behavioural Responses in Budgerigars (Melopsittacus undulatus) Raised in Cages Enriched with Coloured LED Lights
Authors: Ergun Demirel , Taskin Atilla
Published: September 2022

This study examined the effects of exposure to coloured LED lights on some behavioural responses, food and water consumption, and live weight in budgerigars kept in captivity using behavioural tests to compare different lighting conditions. Budgerigars’ feeding, comfort, social, fear, and resting behaviours and behavioural reactions to a new environment were studied. Twelve male birds were assigned to four groups, with three birds in each group. They were provided with food and water ad libitum in wire cages at 25 ± 2 °C in a room. The study was conducted with 10 h of natural light, 6 h of LED light (white, blue, yellow, and red LED lights), and 8 h of darkness. Their behaviours in the cages were observed. Home cage avoidance (HCA) and two open field tests (OFT) were performed. No difference was observed in the LW and food consumption, but the blue and yellow groups consumed less water. The blue group displayed more comfort and social behaviours. In the HCA test, the red group demonstrated higher reactions. Furthermore, the birds in the yellow group stayed closer to the centre of the platform in the OFT I test, and they chose the compartment simulating their familiar environment in the OFT II test. Consequently, it was concluded that first yellow and then blue lights may be used to prevent stress-related problems for these birds. Our findings could be used to improve the hand-rearing conditions of captive budgerigars.