bluuebunny/crossref_metadata_2025 huggingface dataset:
Jeevamrit: A Sustainable Alternative to Chemical Fertilizers for Marigold (Tagetes erecta cv. Siracole) Cultivation under Mid-Hills of Himachal Pradesh
Authors: Kaushal Nitesh , Kashyap Bharati , Bhatia Suman , Kumar Manish , Shah Ali Haidar , Bhardwaj Ragini , Dilta Balbir Singh , Thakur Priyanka
Published: August 2024

Using desi-cow waste products like Jeevamrit under natural farming is widespread among farmers for improving soil biology and productivity. Jeevamrit enhances soil chemical and microbiological properties without needing a large quantity of farmyard manure (FYM) as a sustainable farming practice with a reduced carbon footprint. Despite its traditional use, Jeevamrit faces criticism due to a lack of scientific evidence. This study investigated the comparative effect of Jeevamrit and chemical fertilizers on the growth and yield of marigold cv. Siracole. The experiment employed a randomized block design (RBD) with three replications. The mother block of marigolds was raised for both the summer and winter seasons. From this mother block, three harvesting flushes were taken and propagated from cuttings. The rooted cuttings were planted at monthly intervals and evaluated for flowering parameters and compared to those treated with RDF (30:20:20 N, P, and K g/m2). Soil supplied with Jeevamrit showed enhanced bacteria (26.33%), fungi (18.92%), and actinomycetes (31.21%) populations compared to the recommended dose of fertilizers (RDF) (i.e., N–P–K @ 30:20:20 g m−2). Jeevamrit-treated plants have a more marketable flower yield per square meter (3.98%) and a longer shelf life (9.93%) compared to RDF. The study concludes that Jeevamrit @ 2 liters m−2 is a sustainable and effective alternative to traditional fertilizers for enhancing marigold production in the mid-hills of Himachal Pradesh, where natural farming is already accepted.
Genetic and Phytopathogenic Characterization of Endemic Colletotrichum Isolates in Major Olive Cultivars of Greece
Authors: Angeli Christina , Tsalgatidou Polina C. , Tsafouros Athanasios , Venieraki Anastasia , Zambounis Antonios , Vithoulkas Alexandros , Milionis Anna , Paplomatas Epaminondas J. , Demopoulos Vasilios , Delis Costas
Published: August 2024

Olive anthracnose outbreaks caused by the Colletotrichum species complex in the Mediterranean region decrease both fruit yield and olive oil production while also drastically degrading olive oil quality. The presence of various Colletotrichum species able to produce disease symptoms in olive fruits significantly deteriorates the efforts for an efficient crop protection strategy. In this report, the major olive productive area of Peloponnese was screened for Colletotrichum species capable of generating anthracnose symptoms. Olive fruits of 12 different olive cultivars were collected from 60 groves distributed analogously in the Peloponnese. Thirty-two fungal strains isolated from asymptomatic olive drupes were identified morphologically as Colletotrichum spp. and were multilocus genetically analyzed. The 32 isolates were grouped into two primary lineages resembling the previously characterized Colletotrichum acutatum and Colletotrichum nymphaeae based on the conducted genetic analysis for five genetic loci. The virulence of 16 Colletotrichum spp. strains were evaluated in a detached fruit assay of 10 Greek olive cultivars. The results clearly suggested that fungal isolates belonging to both C. acutatum and C. nymphaeae exhibited different levels of pathogenicity in a cultivar-dependent manner. Thus, cultivars examined in terms of the % Disease Index (%DI) were divided into highly tolerant, tolerant, and susceptible, and those analyzed regarding the % Disease Severity Index (%DSI) were divided into tolerant and susceptible. Our results suggest that the Greek cultivars of Athinolia and Megaritiki are highly tolerant to the vast majority of Colletotrichum strains isolated from Peloponnesian groves and consist of a significant genetic material for the future design of crop protection programs against anthracnose breakouts.
Effects of Temperature Regulation on the Physiological Characteristics and Platycodin Synthesis of Platycodon grandiflorum
Authors: Wang Zhuang , Yan Yan , Han Mei , Yang Limin
Published: August 2024

Platycodon grandiflorum, a dual-purpose herb for food and medicine, is widely distributed in Asia. Although P. grandiflorum has relatively low requirements for its growing environment, temperature remains an important ecological factor affecting its growth, development, and quality formation. In order to explore the effect of different temperatures on P. grandiflorum during their growth period, the diversity in growth physiology, platycodin contents, and gene expression of key enzymes were investigated under constant (8 °C, 18 °C, and 28 °C) and variable (8–18 °C, 8–28 °C, and 18–28 °C) temperature conditions at each of the three levels. The results suggested that both constant and variable temperatures at high levels significantly increased the aboveground fresh weight of P. grandiflorum. However, the low–variable temperature was beneficial for the accumulation of dry and fresh weight in the roots. Regardless of whether temperatures were constant or variable at low levels, this increased the content of soluble sugars, proline, and peroxidase in P. grandiflorum, while upregulating the expression levels of key enzyme genes involved in platycodin synthesis. Meanwhile, a low–constant temperature inhibited the photosynthetic rate of P. grandiflorum. Furthermore, medium–constant and large-scale variable temperatures were conducive to the accumulation of platycodins in the roots. This research provides a theoretical basis and data support for the influence of temperature variations on P. grandiflorum quality formation.
Coastal Almond-Leaved Pear (Pyrus spinosa) Seedlings’ Responses to Saline Stress Alleviated by Formulated L-Methionine and Bacterial Exogenous Soil Application
Authors: Kalorizou Helen , Giannoulis Paschalis , Leontopoulos Stefanos , Angelakis Charalambos , Sorovigka Maria
Published: August 2024

Coastal Pyrus spinosa seedlings were tested for their developmental, chlorophyll content and antioxidant performance under soil saline conditions where bacterial and l-methionine exogenous treatments were applied as potential saline alleviation stress schemes. Scaling up saline stress, the number of formed lateral shoots was reduced in all treatments. Medium salt stress (75 mM NaCl) demonstrated a rather unified decline in shoot fresh weight values, which became toxic at 100 mM NaCl, with up to 89.1% shoot fresh weight losses, in comparison to unchallenged status. Both exogenous applications increased root/shoot ratio, providing developmental boost for root growth. Total chlorophyll content values (May–July) did not differ among non-stressed plantlets independently of exogenous treatment. All experimental plantlet lines increased their antioxidant activity on scaled up soil NaCl enrichment. Νo differences in root orientation and their angle frequencies were observed while soil saline exposure took place. In brief, spring–summer exposure of P. spinosa plantlets under 100 mM NaCl saline stress can be manageable, achieving higher root/shoot ratio values, upregulating leaf antioxidant activity and optimizing root growth upon bacterial and l-methionine supplementation. However, many of the examined parameters were found to be not extensively different between exogenously treated plantlets and non-supplemented ones, suggesting a potential role of intergenerational and transgenerational stress memory.
GSE-YOLO: A Lightweight and High-Precision Model for Identifying the Ripeness of Pitaya (Dragon Fruit) Based on the YOLOv8n Improvement
Authors: Qiu Zhi , Huang Zhiyuan , Mo Deyun , Tian Xuejun , Tian Xinyuan
Published: August 2024

Pitaya fruit is a significant agricultural commodity in southern China. The traditional method of determining the ripeness of pitaya by humans is inefficient, it is therefore of the utmost importance to utilize precision agriculture and smart farming technologies in order to accurately identify the ripeness of pitaya fruit. In order to achieve rapid recognition of pitaya targets in natural environments, we focus on pitaya maturity as the research object. During the growth process, pitaya undergoes changes in its shape and color, with each stage exhibiting significant characteristics. Therefore, we divided the pitaya into four stages according to different maturity levels, namely Bud, Immature, Semi-mature and Mature, and we have designed a lightweight detection and classification network for recognizing the maturity of pitaya fruit based on the YOLOv8n algorithm, namely GSE-YOLO (GhostConv SPPELAN-EMA-YOLO). The specific methods include replacing the convolutional layer of the backbone network in the YOLOv8n model, incorporating attention mechanisms, modifying the loss function, and implementing data augmentation. Our improved YOLOv8n model achieved a detection and recognition accuracy of 85.2%, a recall rate of 87.3%, an F1 score of 86.23, and an mAP50 of 90.9%, addressing the issue of false or missed detection of pitaya ripeness in intricate environments. The experimental results demonstrate that our enhanced YOLOv8n model has attained a commendable level of accuracy in discerning pitaya ripeness, which has a positive impact on the advancement of precision agriculture and smart farming technologies.
Hyperspectral Spatial Frequency Domain Imaging Technique for Soluble Solids Content and Firmness Assessment of Pears
Authors: Yang Yang , Fu Xiaping , Zhou Ying
Published: August 2024

High Spectral Spatial Frequency Domain Imaging (HSFDI) combines high spectral imaging and spatial frequency domain imaging techniques, offering advantages such as wide spectral range, non-contact, and differentiated imaging depth, making it well-suited for measuring the optical properties of agricultural products. The diffuse reflectance spectra of the samples at spatial frequencies of 0 mm-1 (Rd0) and 0.2 mm-1 (Rd0) were obtained using the three-phase demodulation algorithm. The pixel-by-pixel inversion was performed to obtain the absorption coefficient (μa) spectra and the reduced scattering coefficient (μs′) spectra of the pears. For predicting the SSC and firmness of the pears, these optical properties and their specific combinations were used as inputs for partial least squares regression (PLSR) modeling by combining them with the wavelength selection algorithm of competitive adaptive reweighting sampling (CARS). The results showed that μa had a stronger correlation with SSC, whereas μs′ exhibited a stronger correlation with firmness. Taking the plane diffuse reflectance Rd0 as the comparison object, the prediction results of SSC based on both μa and the combination of diffuse reflectance at two spatial frequencies (Rd) were superior (the best Rp2 of 0.90 and RMSEP of 0.41%). Similarly, in the prediction of firmness, the results of μs′, μa×μs′, and Rd1 were better than that of Rd0 (the best Rp2 of 0.80 and RMSEP of 3.25%). The findings of this research indicate that the optical properties represented by HSFDI technology and their combinations can accurately predict the internal quality of pears, providing a novel technical approach for the non-destructive internal quality evaluation of agricultural products.
The TERCAP Tool: Investigation of Nursing Errors in Greek Hospitals
Authors: Pappa Despoina , Evangelou Eleni , Koutelekos Ioannis , Dousis Evangelos , Margari Nikoletta , Toulia Georgia , Stavropoulou Areti , Koreli Alexandra , Theodoratou Maria , Bilali Aggeliki , Chasaki Konstantina , Zartaloudi Afroditi , Dafogianni Chrysoula
Published: August 2024

Background: Errors are a common occurrence in all healthcare settings, and the safety of patients is a critical concern that involves multiple factors, including the complex and demanding nature of nursing practice. Nurses, due to their continuous and direct patient care, play a pivotal role in ensuring patient safety. This cross-sectional study aimed to investigate the factors that contribute to errors from the perspectives of nurses in Greek hospitals, with a focus on understanding the challenges they face in their daily practice. Methods: Clinical nurses willingly and anonymously filled out a specific structured questionnaire, the Taxonomy of Error, Root Cause Analysis, and Practice-responsibility (TERCAP) tool that describes the conditions under which an error during clinical practice occurred. The study method included convenience sampling. After obtaining permission, questionnaires were distributed to hospital departments. To accommodate pandemic-related restrictions, an electronic version of the questionnaire was also created for distribution and collection. Analysis of data was accomplished via SPSS 26.0. Results: Five hundred and ninety-seven clinical nurses participated anonymously, reporting errors in almost seven out of ten cases, often attributing them to high workload and staff shortages. Errors were commonly reported during different shifts in this study. Factors such as assigning significant responsibilities to inexperienced staff and inadequate implementation guidelines were highlighted. Conclusions: The in-depth study of nursing errors provides a nuanced understanding of their causes by categorizing them based on various factors. It emphasizes the complexity of challenges and the need to integrate systemic, clinical, and individual factors into intervention strategies, including medication protocols, ongoing training, clear communication, administrative support, and fostering an open communication culture.
A Review of Winter Ulcer Disease and Skin Ulcer Outbreaks in Atlantic Salmon (Salmo salar)
Authors: Ghasemieshkaftaki Maryam
Published: August 2024

Winter ulcer disease (WUD) is widely recognized as a serious threat to animal welfare and a major contributor to revenue loss within the aquaculture sector, particularly affecting the salmon-farming industry. This highlights the significant impact of WUD on both animal well-being and the economic sustainability of fish farming. WUD causes hemorrhagic signs and results in dermal lesions and ulcers. This disease can lead to higher mortality rates and a considerable decline in the fish’s market value. Moritella viscosa, a Gram-negative bacterium, is predominantly, but not exclusively, correlated with the emergence of WUD, mostly during the colder seasons. Waterborne transmission is the primary way for spreading the bacterium within a population. However, there is remarkable variation in the prevalence and characteristics of WUD in different regions. In Europe, this disease often occurs in the winter, and the intensity and occurrence of outbreaks are influenced by water temperature and salinity. In contrast, outbreaks are typically observed in the summer and mid-autumn in Eastern Canada. Despite the administration of various polyvalent vaccines, outbreaks of skin ulcers have been documented in Canada, and studies have highlighted the possible roles of other bacterial pathogens in Atlantic salmon. This review discusses the etiology, pathogenesis, and potential mitigation or prevention strategies for WUD, mainly in Atlantic salmon. Moreover, it underscores the necessity of conducting further investigations to discover the potential unknown causative agents of ulcerative disease and design appropriate vaccines or preventive strategies for these pathogens.
Systematic Review of Presymptomatic Treatment for Spinal Muscular Atrophy
Authors: Cooper Katy , Nalbant Gamze , Sutton Anthea , Harnan Sue , Thokala Praveen , Chilcott Jim , McNeill Alisdair , Bessey Alice
Published: August 2024

Spinal muscular atrophy (SMA) causes the degeneration of motor neurons in the spinal cord. Treatments including nusinersen, risdiplam, and onasemnogene abeparvovec have been shown to be effective in reducing symptoms, with recent studies suggesting greater effectiveness when treatment is initiated in the presymptomatic stage. This systematic review synthesises findings from prospective studies of presymptomatic treatment for 5q SMA published up to December 2023. The review identified three single-arm interventional studies of presymptomatic treatment (NURTURE, RAINBOWFISH, and SPR1NT), six observational studies comparing presymptomatic or screened cohorts versus symptomatic cohorts, and twelve follow-up studies of screened cohorts only (i.e., babies identified via newborn screening for SMA). Babies with three SMN2 copies met most motor milestones in the NURTURE study of nusinersen and in the SPR1NT study of onasemnogene abeparvovec. Babies with two SMN2 copies in these two studies met most motor milestones but with some delays, and some required ventilatory or feeding support. The RAINBOWFISH study of risdiplam is ongoing. Naïve comparisons of presymptomatic treatment in SPR1NT, versus untreated or symptomatic treatment cohorts, suggested improved outcomes in patients treated presymptomatically. Comparative observational studies supported the finding that presymptomatic treatment, and early treatment following screening, may improve outcomes compared with treatment at the symptomatic stage. Further research should assess the long-term clinical outcomes and cost-effectiveness of presymptomatic treatment for SMA.
Advanced Laser–Plasma Diagnostics for a Modular High-Repetition-Rate Plasma Electron Accelerator
Authors: Greb Christian , Aktan Esin , Adam Roman , Dickson Alex , Sire Cédric , Nefedova Viktoria E. , Sylla François , Lopez-Martens Rodrigo , Schneider Claus M. , Faure Jérôme , Büscher Markus
Published: August 2024

We present a laser–plasma electron accelerator module designed to be driven by high-repetition-rate lasers for industrial applications of laser-driven electron beams. It consists of a single vacuum chamber containing all the necessary components for producing, optimizing, and monitoring electron beams generated via laser wakefield acceleration in a gas jet when driven by a suitable laser. The core methods in this paper involve a comprehensive metrological assessment of the driving laser by rigorous temporal laser pulse characterization and contrast measurements, supplemented by detailed spatiotemporal distribution analyses of the laser focus. Results demonstrate the good stability and reproducibility of the laser system, confirming its suitability for advanced scientific and industrial applications. We further demonstrate the functionality of the laser–plasma accelerator module diagnostics, perform target density characterizations, and time-resolved laser–plasma shadowgraphy. Current limitations of the set-up preventing first electron acceleration are analyzed and an outlook for future experiments is given. Our work is a first step towards the wide dissemination of fully integrated laser–plasma accelerator technology.
Efficiency in Kinesiology: Innovative Approaches in Enhancing Motor Skills for Athletic Performance 2.0
Authors: Sorgente Vincenzo , Minciacchi Diego
Published: August 2024

The second edition of the Special Issue titled “Efficiency in Kinesiology: Innovative approaches in enhancing motor skills for Athletic Performance” has been effectively concluded, significantly enriching the discourse on “efficiency in kinesiology” by presenting a diverse array of innovative research findings and methodologies aimed at optimizing athletic performance and motor-skill development (https://www [...]
Simultaneous Stereo Matching and Confidence Estimation Network
Authors: Schmähling Tobias , Müller Tobias , Eberhardt Jörg , Elser Stefan
Published: August 2024

In this paper, we present a multi-task model that predicts disparities and confidence levels in deep stereo matching simultaneously. We do this by combining its successful model for each separate task and obtaining a multi-task model that can be trained with a proposed loss function. We show the advantages of this model compared to training and predicting disparity and confidence sequentially. This method enables an improvement of 15% to 30% in the area under the curve (AUC) metric when trained in parallel rather than sequentially. In addition, the effect of weighting the components in the loss function on the stereo and confidence performance is investigated. By improving the confidence estimate, the practicality of stereo estimators for creating distance images is increased.
Adoption of a Societal Perspective in Economic Evaluations of Musculoskeletal Disorders: A Conceptual Paper
Authors: Fatoye Francis , Gebrye Tadesse , Nherera Leo , Trueman Paul
Published: August 2024

Economic evaluations are used to compare the costs and consequences of healthcare interventions, including those for musculoskeletal (MSK) disorders, which are very common and a major source of morbidity and absence from work. Reimbursement decisions for interventions for MSK disorders by decision-makers rely on the findings of economic evaluations, the design and results of which depend largely on the perspective adopted. Despite methodological advancements in economic evaluations, there are no clear guidelines on the perspective to adopt. This paper explores the adoption of a societal perspective in economic evaluations of MSK disorders. Within health economics evaluations, the most commonly used perspectives include the payer perspective, the healthcare perspective, and the societal perspective. To facilitate optimal resource allocation decisions in order to reduce the significant economic burden of MSK disorders and improve the health outcomes of individuals with these disorders, all costs and benefits associated with interventions for them should be included. Thus, the societal perspective is arguably a preferable option to the others for economic evaluations of interventions for MSK disorders.
Design and Principles Analysis of Hydrofoil Appendages for Reducing Resistance of High-Speed Ships
Authors: Chen Qian , Liu Zhihua , Liu Wentao , Zhao Gangquan
Published: August 2024

To reduce the resistance of high-speed displacement ships with Froude numbers (Fr) between 0.4 and 0.5, this paper proposes the installation of hydrofoils at the bow and stern of the ship. Firstly, starting from the bow wave, this paper proposes the installation of a flat plate appendage at the free surface of the ship’s bow to suppress the height of the bow wave and thus reduce the hull resistance. Taking the DTMB 5415 ship model as the research object, CFD calculation results show that installing a flat plate appendage at the free surface of the ship’s bow can effectively suppress the height of the bow wave, and the total resistance reduction ratio can reach 6.49% when Fr = 0.45. Then, the flat plate appendage was improved to a hydrofoil appendage, further reducing the hull resistance. As a result, the total resistance reduction rate can reach 9.15% at Fr = 0.45. Following this, hydrofoil appendages were installed simultaneously at the bow and stern. The drag reduction effect and mechanism were studied, and the results show that the hydrofoils at the bow and stern have a good drag reduction effect. Suppressing the bow and stern waves and improving the flow field are the main reasons for the drag reduction. Finally, the drag reduction effect of the hydrofoil appendages was verified through experiments, demonstrating its excellent drag reduction effect when Fr = 0.4–0.5 and a maximum total resistance reduction ratio of 14.552%.
Spatio-Temporal Compressive Behaviors of River Pebble Concrete and Sea Pebble Concrete in Island Offshore Engineering
Authors: Yuan Yuan , Zhao Yian , Feng Xiaotian , Lei Yanhua , Zhang Wenbing
Published: August 2024

Obtaining river or sea pebbles from local resources for concrete production is considered an economical and eco-friendly alternative, particularly in marine and island-offshore engineering. However, the resulting changes in the mechanical properties of these concrete have attracted attention. This study investigates the compressive behavior of concretes where river or sea pebbles partially (i.e., 33% and 67%) or fully (i.e., 100%) replace traditional gravel as coarse aggregate, using a noncontact full-field deformation measurement system based on digital image correlation (DIC). Compared to the traditional gravel concrete (GC), compressive strengths of the river pebble concrete (RPC) at constitution rates of 33%, 67%, and 100% decreased by 6.5%, 29.8%, and 38.9% while those values of the sea pebble concrete (SPC) decreased by 13.1%, 32.7%, and 44.3%, respectively. Meanwhile, SPC exhibited slightly lower compressive strength than RPC. The peak strains of both SPC and RPC decreased at lower substitution rates, although their stress-strain curves resembled those of GC. In contrast, RPC and SPC at higher substitution rates exhibited a noticeable stage of load hardening. Full-field deformation data and interfacial characteristics indicated that the compressive failure modes of both RPC and SPC showed significant interfacial slipping between pebbles and mortar with increasing coarse aggregate substitution rates. In comparison, fractures in coarse aggregate and mortar were observed in damaged GC. The study demonstrated that the spatio-temporal compressive deformation response and failure modes of SPC and RPC were distinct due to the introduction of pebbles, providing insights for engineering applications of river/sea pebble concrete in practical offshore or island construction projects.
Occurrence and Characteristics of Serpula himantioides Fruiting Bodies on Living Trees in Japan
Authors: Haraguchi Ryusei , Hirao Toshihide , Yamada Toshihiro
Published: August 2024

Serpula himantioides is a globally distributed wood decay fungus that causes heartwood decay in several tree species. We investigated the occurrence of S. himantioides fruiting bodies in Japan for two years and six months to characterize their biology. The fruiting bodies matured in autumn and occurred on living Chamaecyparis pisifera, Chamaecyparis obtusa, Larix kaempferi, and Cryptomeria japonica trees, as well as on dead trees and soil. Assessing three circular plots, the incidence of living trees with S. himantioides fruiting bodies was lowest in the plot with the most advanced heartwood decay. Furthermore, fruiting bodies occurred more frequently in the lower slope direction of the trunk. Analysis using the pair correlation function suggested that the spatial distribution pattern of living trees with fruiting bodies may change from intensive to random with heartwood decay progress. Finally, according to generalized linear and generalized linear mixed models, which were used to investigate the factors affecting the development of fruiting bodies in C. pisifera, C. obtusa, and L. kaempferi, no clear relationship was found between the presence or absence of fruiting bodies and heartwood decay. Thus, we suggest that fruiting bodies can occur in healthy living trees as well as in living trees in the early stages of heartwood decay.
Isolation of Antagonistic Endophytic Fungi from Postharvest Chestnuts and Their Biocontrol on Host Fungal Pathogens
Authors: Wen Yunmin , Li Meng , Yang Shuzhen , Peng Litao , Fan Gang , Kang Huilin
Published: August 2024

In this study, antagonistic endophytic fungi were isolated from postharvest chestnut fruits; endophytic antagonistic fungi and their combination of inhibitory effects on the fungal pathogen Neofusicoccum parvum were evaluated. A total of 612 endophytic fungi were isolated from 300 healthy chestnut kernels, and 6 strains out of them including NS-3, NS-11, NS-38, NS-43, NS-56, and NS-58 were confirmed as antagonistic endophytic fungi against Neofusicoccum parvum; these were separately identified as Penicillium chermesinum, Penicillium italicum, Penicillium decaturense, Penicillium oxalicum, Talarmyces siamensis, and Penicillium guanacastense. Some mixed antagonistic endophytic fungi, such as NS-3-38, NS-11-38, NS-43-56, and NS-56-58-38, exhibited a much stronger antifungal activity against N. parvum than that applied individually. Among them, the mixture of NS-3-38 showed the highest antifungal activity, and the inhibition rate was up to 86.67%. The fermentation broth of NS-3, NS-38, and their combinations exhibited an obvious antifungal activity against N. parvum, and the ethyl acetate phase extract of NS-3-38 had the strongest antifungal activity, for which the inhibitory rate was up to 90.19%. The NS-3-38 fermentation broth combined with a chitosan coating significantly reduced N. parvum incidence in chestnuts from 100% to 19%. Furthermore, the fruit decay and weight loss of chestnuts during storage were significantly decreased by the NS-3-38 fermentation broth mixture along with a chitosan coating. Therefore, a mixture of P. chermesinum and P. decaturense could be used as a potential complex biocontrol agent to control postharvest fruit decay in chestnuts.
Assessment of Genetic Diversity and the Population Structure of Species from the Fusarium fujikuroi Species Complex Causing Fusarium Stalk Rot of Maize
Authors: Jambhulkar Prashant P. , Bajpai Ruchira , Reddy Harish Jayarama , Tripathy Partha Sarathi , Varun Priyanka , Rout Ajaya Kumar , Behera Bijay Kumar , Lakshman Dilip K. , Nanjundappa Mallikarjuna
Published: August 2024

Fusarium stalk rot (FSR), caused by the Fusarium species complex, is an economic threat to maize cultivation all over the world. We investigated the population structure and genetic diversity of Fusarium species obtained from five major maize-growing regions in India. The Tef-1α locus was used for phylogenetic analysis of geographically distinct isolates of Fusarium verticillioides, F. andiyazi, F. proliferatum, F. nygamai, and F. acutatum causing FSR. A phylogenetic tree showed monophyletic, polyphyletic, and paraphyletic groupings reflecting the complex evolutionary history and genetic diversity within the genus. Monophyletic groupings depicting strong bootstrap support were shown to have a single common ancestor and genetic coherence with limited genetic divergence among sequences. Polyphyletic groupings also presented significant genetic differentiation within the F. verticillioides sequences from diverse ecological niches. Nucleotide diversity of moderate level 0.02471 reflected genetic variations within populations that were attributed to factors such as mutation, genetic drift, or varying selection pressures. The Fst value of 0.98205 is particularly indicative of high genetic differentiation, implying that most of the genetic variance is due to differences between populations rather than within them. F. verticillioides, with 57 sequences, showed low genetic diversity with three segregating sites and a low haplotype diversity of 0.19486, suggesting the founder effect, where a reduced population expands from a limited genetic pool. The total data estimates across all populations for haplotype analysis showed 72 sequences, 44 segregating sites, and 9 haplotypes with a haplotype diversity of 0.48513. The evolutionary dynamics showed genetic differentiations among Fusarium species causing FSR. AMOVA indicated high within-population variations, depicting a substantial genetic diversity within individual populations. The results offer a comprehensive framework for discussing the implications of genetic diversity in pathogen management and the evolutionary dynamics of the Fusarium species causing FSR in maize in the Indian subcontinent.
Reduced Clavicle Length Indicates the Severity of Scapular Misalignment in Obstetric Brachial Plexus Lesions
Authors: Rosenauer Rudolf , Nógrádi Antal , Quadlbauer Stefan , Schmidhammer Markus , Schmidhammer Robert , Tsolakidis Savas
Published: August 2024

(1) Background: Although most brachial plexus birth palsies show some spontaneous recovery, secondary operations are likely to follow. Accordingly, due to the loss of muscle innervation, the growth of the affected limb and the shoulder girdle is reduced. This is associated with pathological scapula positioning and rotation. The objective of this work was to clarify the relationship between length differences of the two clavicles and different types of scapular dyskinesia. (2) Methods: Twenty-five patients suffering from brachial plexus birth palsy were included in this retrospective study. There were eighteen female and seven male patients with a mean age of 10 years (2 to 23 years). CT scans of the thoracic cage, including both shoulder joints and both clavicles, were obtained preoperatively between 2010 and 2012. Radiographic measurements were taken of the axial plane and 3D reconstructions were produced. Functional evaluations of possible movement and scapular dyskinesia were performed. (3) Results: We found an increasing difference in the length of the clavicle (both in absolute and relative terms) in the children with more pronounced scapular dyskinesia. Additionally, with increasing clavicle length differences, the scapula was positioned in a deteriorated angle compared to the healthy side. Significant positive correlations were identified for the age and absolute difference of the clavicle length and the length and width of the scapula on the affected side. (4) Conclusion: Scapular dyskinesia, which is a common finding in brachial plexus birth palsy, is strongly related to reduced clavicle growth. Reduced clavicle length (which is a relatively easily examinable parameter) compared to the healthy side can be used to estimate the extent of scapular malpositioning on the thoracic cage. The extent and severity of scapular dyskinesia increases with augmented differences in the length of the clavicle.
RIAIT (Italian Registry of Allergen Immunotherapy): Protocol for a New Tool in a New Vision of Disease-Modifying Therapy for Allergists
Authors: Costanzo Giovanni , Caruso Cristiano , Paoletti Giovanni , Baglivo Ilaria , Colantuono Stefania , Bagnasco Diego , Caminati Marco , Giovannini Mattia , Castagnoli Riccardo , Senna Gianenrico , Sirena Concetta , Tosca Maria Angela , Passalacqua Giovanni , Marseglia Gian Luigi , Miraglia del Giudice Michele , Ciprandi Giorgio , Indolfi Cristiana , Barberi Salvatore , Landi Massimo , Di Gioacchino Mario , Canonica Giorgio Walter , Heffler Enrico
Published: August 2024

Randomized controlled trials have demonstrated responses to clinical parameters, but a significant proportion of allergy patients in real-life settings would have been excluded from such studies. Therefore, real-world research is needed, and there is a growing body of information on allergen immunotherapy’s long-term effectiveness and safety. Real-world evidence can be a valuable instrument to better understand the patient’s journey and the effectiveness and safety of therapies. For this purpose, a registry will be used for the first time in Italy to evaluate the impact of allergen immunotherapy on several outcomes, including quality of life and disease-related effects in the pediatric and adult allergic population with a socio-economic assessment and respect to real-world health.
AI-Driven Thoracic X-ray Diagnostics: Transformative Transfer Learning for Clinical Validation in Pulmonary Radiography
Authors: Sufian Md Abu , Hamzi Wahiba , Sharifi Tazkera , Zaman Sadia , Alsadder Lujain , Lee Esther , Hakim Amir , Hamzi Boumediene
Published: August 2024

Our research evaluates advanced artificial (AI) methodologies to enhance diagnostic accuracy in pulmonary radiography. Utilizing DenseNet121 and ResNet50, we analyzed 108,948 chest X-ray images from 32,717 patients and DenseNet121 achieved an area under the curve (AUC) of 94% in identifying the conditions of pneumothorax and oedema. The model’s performance surpassed that of expert radiologists, though further improvements are necessary for diagnosing complex conditions such as emphysema, effusion, and hernia. Clinical validation integrating Latent Dirichlet Allocation (LDA) and Named Entity Recognition (NER) demonstrated the potential of natural language processing (NLP) in clinical workflows. The NER system achieved a precision of 92% and a recall of 88%. Sentiment analysis using DistilBERT provided a nuanced understanding of clinical notes, which is essential for refining diagnostic decisions. XGBoost and SHapley Additive exPlanations (SHAP) enhanced feature extraction and model interpretability. Local Interpretable Model-agnostic Explanations (LIME) and occlusion sensitivity analysis further enriched transparency, enabling healthcare providers to trust AI predictions. These AI techniques reduced processing times by 60% and annotation errors by 75%, setting a new benchmark for efficiency in thoracic diagnostics. The research explored the transformative potential of AI in medical imaging, advancing traditional diagnostics and accelerating medical evaluations in clinical settings.
Radiation-Associated Angiosarcoma of the Breast: The State of the Art of a Rare and Aggressive Disease
Authors: Cozzi Salvatore , Finocchi Ghersi Sebastiano , Tava Francesca , Bardoscia Lilia , Najafi Masoumeh , Ruggieri Maria Paola , Serre Anne-Agathe , Roukoz Camille , Gutierrez Miguelez Cristina , Lazrek Amina , Sardaro Angela , Taverna Cecilia
Published: August 2024

Introduction: Considering the increasing number of conservative surgeries (quadrantectomies) for primary breast carcinoma, especially in the early stages, often followed by adjuvant radiotherapy, the incidence of radiation-associated angiosarcoma (RAS) is expected to rise in the coming decades, and it will represent a clinical and therapeutic challenge, as limited data are available due to the rarity and heterogeneity of the disease. Though the prognosis of these patients is poor, a number of clinical and pathological factors can be evaluated to better understand the course of RAS. The aim of this systematic review is to explore the available clinical-pathological, therapeutic, and prognostic data regarding RAS to evaluate its occurrence, diagnosis, treatment, and outcomes. Method: RAS clinical data were identified by a systematic review conducted in five different databases (Medline, Web of Knowledge, Google Scholar, Scopus, and Cochrane). Only RAS works published in English, with access to the full manuscript text, and with clear results, were considered as eligible. Results: We considered 52 papers comprising 319 RAS cases. The patient age at diagnosis ranged from 37 to 93 years, with most cases occurring from 5 to 10 years after breast irradiation. The most common clinical presentation was an aggressive development of macules, papules, or erythematous-violaceous skin discoloration at the site of previous radiation treatment for primary breast carcinoma. Complete surgical excision appeared to be the first-line treatment of the disease, in some cases followed by adjuvant local and/or systemic therapies. Despite different treatments, local recurrence rates ranged from 40% to 90%, leading to negative outcomes and poor prognosis for patients. Conclusion: Although the literature is limited and the data are heterogeneous and contentious, our review aims to highlight the importance of early diagnosis, multimodal treatment, and long-term follow-up of RAS in order to limit and prevent the aggressiveness of this neoplasm.
The Effects of Monitoring Activities on Loan Defaults in Group-Based Lending Program: Evidence from Vietnam
Authors: Ba-Tri Tran , Truong Loc Dong , Friday H. Swint , Pham Tien Phat
Published: August 2024

The aim of this study is to investigate the impact of delegated monitoring by a group leader and peer monitoring by group members on loan defaults in a group-based lending program in Vietnam. The data used in the study were collected from a questionnaire survey of 675 participants involved in a group-based lending program conducted from August to October 2022 in the Mekong River Delta, Vietnam. This group-based lending program employs a unique monitoring system that involves hiring the group leader to supervise the group and encouraging group members to monitor each other. The empirical findings derived from the Probit model indicated that delegated monitoring significantly reduces loan defaults, but there was no evidence supporting the effectiveness of peer monitoring within the group. Additionally, under the delegated monitoring scheme, commissions and group size plays an important role in decreasing loan defaults. The implication of the findings is that the Vietnam Bank for Social Policies (VBSP) could maintain large group sizes to provide incentives for group leaders through commissions to enhance repayment rates.
Coprological Survey of Helminths in Reindeer (Rangifer tarandus) in 50 Selected Zoos and Menageries in Russia
Authors: Loginova Olga A. , Akulova Svetlana V. , Egorov Dmitry N. , Egorova Natalia S. , Spiridonov Sergei E. , Plotnikova Iuliia K. , Belova Larisa M. , Kuznetsov Yuriy E. , Chuprak Daria I. , Krutikova Anna A. , Vasilkova Iuliia V. , Gelashvili Diana A. , Shchepanovsky Yuri A. , Mizin Ivan A. , Panchenko Danila V. , Bondar Mikhail G. , Sipko Taras P.
Published: August 2024

Zoo conditions are unique for reindeer, since domestic reindeer are not kept in captive facilities like cattle. In the zoo, reindeer are usually surrounded by many different animals that they would never encounter naturally. Thus, they might be infected with new helminths. Numerous petting zoos raise concerns about the safety of tactile interactions for human visitors. Our study is the first large-scale one. Qualitative and quantitative fecal analyses were carried out for 233 reindeer distributed over 50 Russian zoos according to the National Standard of the Russian Federation (GOST R 54627-2011) Ruminant animals—Methods of Laboratory Helminthological Diagnostics. Where possible, DNA analyses of helminths were performed targeting internal transcribed spacer region. As a result, F. hepatica, Paramphistomum sp., Moniezia sp. (including M. expansa), gastrointestinal strongylids (including Nematodirus spp.), Dictyocaulus sp., E. rangiferi, Trichuris sp., and Capillaria sp. were found in 106 (45%) zoo reindeer. All these helminths were previously reported in reindeer and pose no direct danger for humans. The intensity of invasions was mostly low. Fecal examination might be considered as an indirect method for mange diagnostics, as Chorioptes and Demodex mites were found in reindeer fecal samples. The latter may represent a novel species of mite specific for reindeer.
Evaluation of the Importance of Ecological Service Function and Analysis of Influencing Factors in the Hexi Corridor from 2000 to 2020
Authors: Liu Yidan , Yao Xiaojun , Tian Zhijuan , Zhang Yingying
Published: August 2024

The Hexi Corridor plays a pivotal role in safeguarding China’s ecological security, functioning as a crucial conduit between economic and ecological systems. This study employs a multidisciplinary approach, integrating climate, evapotranspiration, and other variables, to analyze the trend in factors influencing ecological function and to evaluate the import of ecological service functions in the Hexi Corridor from 2000 to 2020. The findings reveal a distinctive spatial distribution pattern for regulating functions, with higher concentrations observed in the southern regions and lower concentrations in the northern regions. These functions include the storage of carbon, quality of habitat, the conservation of water, and soil and water conservation. It can be observed that the areas of general importance for ecosystem services are predominantly distributed across the northern and western sections of the Hexi Corridor, collectively representing 76.96% of the total area. Conversely, areas of general importance for ecosystem services are situated in regions characterized by a high altitude, intricate topography, and extensive glaciers as well as permafrost.
Spatiotemporal Accessibility of Rail Transport Systems in the Guangdong–Hong Kong–Macao Greater Bay Area and Its Implications on Economic Equity
Authors: Ouyang Shishu , Zhao Pengjun , Gong Zhaoya
Published: August 2024

Reducing inequality and fostering economic growth is the tenth global goal of the United Nations for sustainable development. Rail transport significantly influences spatial structures, industrial distributions, and is vital for regional economic integration. Despite its importance, the impact of rail transport on economic equity has not been thoroughly explored in current literature. This study aims to fill this gap by evaluating the spatiotemporal characteristics of rail transport accessibility and its implications for economic equity in the Guangdong–Hong Kong–Macao Greater Bay Area. We considered high-speed, intercity, and conventional rail transport and employ three distinct indicators—door-to-door travel time, weighted average travel time, and potential accessibility—to provide a nuanced assessment of accessibility in the region. Each indicator provides a unique perspective on how accessibility affects economic equity, collectively broadening the scope of the analysis. From 1998 to 2020, the evolution of rail transport and its consequent impact on regional economic equity is scrutinized. Advanced econometric methods, namely ordinary least squares, and spatial Durbin models, are combined with the Gini coefficient and Lorenz curve for comprehensive quantitative analysis. This approach highlights the dynamic influence of rail transport development on economic equity, contributing to the sustainable urban development discourse. The results reveal that although rail transport advancements bolster connectivity and economic growth, they also exacerbate regional economic inequality. This study provides valuable insights for urban planning and policymaking by elucidating the complex relationship between rail transport accessibility and economic equity. Our findings underscore the importance of implementing balanced and inclusive transport policies that foster growth and efficiency while mitigating socioeconomic disparities.
A Case Study of Negated Adjectives in Commuters’ Twitter Complaints
Authors: Ruytenbeek Nicolas
Published: August 2024

In today’s digital society, social networks such as Twitter are a preferred place for expressing one’s emotions, especially when they are negative. Despite a growing interest in the variety of linguistic realizations of commuters’ complaints, little attention has so far been paid to writers’ choices, especially when morphologically or syntactically simpler alternative formulations are available. A typical example is the “inference towards the antonym” triggered by the negation of contrary adjectives, an effect that is stronger for positive compared to negative adjectives. In the context of railway transport, a customer could use the negative statement The train is not clean instead of the corresponding affirmative sentence The train is dirty. It remains unclear, in our current state of knowledge, why online customers would prefer more complex constructions to voice their criticisms. Based on a large corpus of tweets sent to the French and Belgian national railway companies by their customers, I have semi-automatically extracted instances of not (very) + adjective (ADJ). Based on previous observations in the literature, I expected positive adjectives to be more frequently used in these negative environments compared to negative ones. As recent research demonstrates that one’s desire to save the interlocutor’s face is not necessarily the only reason why positive adjectives are used in linguistically negative environments, other motivations will also be considered. More precisely, I suggest that in a context where negativity is prevalent, customers using negated positive adjectives kill two birds with one stone: not only do they signal an issue with a product or a service, pointing to expectations that have not been met by the company, but they also mitigate the impact of their negative comments to the positive face of the service managers with whom they are interacting. By offering a quantitative, corpus-based analysis of negative constructions, complemented by a qualitative linguistic analysis of selected examples, this research sheds new light on users’ lexical choices in online negative customer feedback.