bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Grain Chemical Composition, Ruminal Degradation In Vivo, and Intestinal Digestibility In Vitro of Vicia Sativa L. Varieties Grown on the Tibetan Plateau
Authors: Huang Yafeng , Li Rui , Coulter Jeffrey A. , Zhang Zhixin , Nan Zhibiao
Published: May 2019

Four varieties of common vetch, three improved varieties and one local variety, were evaluated for grain chemical composition, rumen protein degradability, and intestinal protein digestibility over two cropping years on the Tibetan Plateau. This study also examined correlations of grain chemical composition with rumen degradability parameters of grain protein and with intestinal digestibility of grain protein. Results of this study showed that grain quality attributes varied (p < 0.05) among varieties and cropping years. Significant intra-species variation was observed for concentrations (g/kg dry matter) of crude protein (CP; range = 347–374), ether extract (range = 15.8–19.6), neutral detergent fiber (aNDF; range = 201–237), acid detergent fiber (range = 58.2–71.6), ash (range = 27.6–31.0), effective CP degradability (EDCP; range = 732–801 g/kg CP), and intestinally absorbable digestible protein (IADP; range = 136–208 g/kg CP). The relationship between grain chemical composition and IADP was best described by the linear regression equation IADP = –0.828CP + 8.80ash + 0.635aNDF + 70.2 (R2 = 0.891), indicating that chemical analysis offers a quick and reliable method for IADP of common vetch grain. In terms of EDCP and IADP of grain, common vetch varieties, Lanjian No.2 and Lanjian No. 3, have the greatest potential among varieties tested for supplementing ruminant diets when grown on the Tibetan Plateau.
Slag as an Inventory Material for Heat Storage in a Concentrated Solar Tower Power Plant: Design Studies and Systematic Comparative Assessment
Authors: Krüger Michael , Haunstetter Jürgen , Knödler Philipp , Zunft Stefan
Published: May 2019

By using metallurgical slag from an electric arc furnace that is otherwise not recycled but deposited as an inventory material in thermal energy storage for concentrated solar power plants, it is possible to make a significant step forward in two transformation processes: energy and raw materials. As this type of slag has not been considered as an inventory material for this purpose, it is important to clarify fundamental questions about this low-cost material and its storage design. In this paper, design studies of slag-based thermal energy storage are carried out. Different slag-specific design concepts are developed, calculated and evaluated by a method based on established management tools. Finally, concepts for further investigations are defined. The highest aptitude value and the lowest risk value are achieved by the vertical storage design with axial flow direction. Therefore, it is taken as the lead concept and will be considered in complete detail in further research. Also, a closer look, but not as detailed as the lead concept, is taken at the horizontal storage with axial flow and the vertical storage with radial flow direction.
Effect of the Dynamic Cone Angle on the Atomization Performance of a Piezoceramic Vibrating Mesh Atomizer
Authors: Yan Qiufeng , Wu Chuanyu , Zhang Jianhui
Published: May 2019

In this paper, we find that the dynamic cone angle of a piezoceramic atomizer is linked to periodic changes in the volume of the micro-cone hole of the atomizer, and such changes affect atomization performance. Firstly, we explained the theory of the dynamic cone angle inside the vibrating mesh atomizer. Then, we analyzed the flow status of liquid in the micro-cone hole, and the one-way flow Rof the liquid is caused by the difference of diffuser and nozzle flow resistance. The volume change of the micro-cone hole and the liquid chamber can produce atomization. Furthermore, we developed the experiment to measure the atomization rate, atomization height, and the diameter of the atomized particles. The experiments reveal that the atomization rate and height are much larger when the vibrating mesh atomizer is working in the forward path than in the reverse one. The atomization rate and atomization height increase as the working voltage increases. Meanwhile, with increasing driving voltage to the piezoceramic actuator, the atomization particle size decrease and the atomized particle size distribution is more concentrated. Finally, the size of the micro-cone hole was measured using a microscope with different direct current (DC) voltages, further demonstrating the existence of the dynamic cone angle.
mTOR Inhibitors Can Enhance the Anti-Tumor Effects of DNA Vaccines through Modulating Dendritic Cell Function in the Tumor Microenvironment
Authors: Chen Yu-Li , Lin Han-Wei , Sun Nai-Yun , Yie Jr-Chi , Hung Hsueh-Chih , Chen Chi-An , Sun Wei-Zen , Cheng Wen-Fang
Published: May 2019

The life span of dendritic cells (DCs) can become short following induced activation, which is associated with metabolic transition due to the regulation of mechanistic target of rapamycin (mTOR). The purpose of this study was to investigate the potential of inhibiting mTOR to modulate DC functions for elevating the anti-tumor effects of DNA vaccines. Therefore, the influences of various inhibitors of mTOR (mTORi) on the expressions of DC maturation markers, the abilities of antigen presenting and processing of BMM-derived DCs and the tumor killing effects of E7-specific CD8+ T lymphocytes activated by BMM-derived DCs were in vitro examined. The anti-tumor effects of connective tissue growth factor (CTGF)/E7 DNA vaccine and/or mTORi were also in vivo analyzed. In our study, suppressive effects of mTORi on the DC maturation markers expressed on BMMCs could be reversed. The mTORi-treated mature BMM-derived DCs tended to be non-apoptotic. These mTORi-treated BMM-derived DCs could have better antigen presenting and processing abilities. The E7-specific cytotoxic CD8+ T lymphocytes could have more potent tumoricidal activity following activation of mTORi-treated BMM-derived DCs. For tumor-bearing mice, those treated with CTGF/E7 DNA vaccine and mTORi indeed can have higher percentages of mature DCs in the TME, better disease control and longer survivals. Consequently, application of mTORi can be a pharmacological approach for temporally increasing life span, antigen presenting and antigen processing of DCs to strengthen the therapeutic outcome of cancer immunotherapy.
Cardiovascular Organ Damage and Blood Pressure Levels Predict Adverse Events in Multiple Myeloma Patients Undergoing Carfilzomib Therapy
Authors: Bruno Giulia , Bringhen Sara , Maffei Ilaria , Iannaccone Andrea , Crea Teresa , Ravera Agnese , Astarita Anna , Vallelonga Fabrizio , Salvini Marco , Gay Francesca , Veglio Franco , Milan Alberto
Published: May 2019

Carfilzomib is a second-generation proteasome inhibitor approved for the treatment of multiple myeloma (MM). It seems to determine cardiovascular toxicity, primarily arterial hypertension. No predictive factors for cardiovascular adverse events (CVAEs) are known in patients affected by multiple myeloma treated with carfilzomib. We evaluated the role of cardiovascular organ damage parameters to predict CVAEs in MM patients taking carfilzomib. Seventy patients affected by MM were prospectively enrolled. A comprehensive cardiovascular evaluation was performed before carfilzomib therapy; they underwent a transthoracic echocardiogram and the assessment of carotid-femoral pulse wave velocity. All the patients were followed up (FU) to determine the incidence of CVAEs. The mean age was 60.3 ± 8.2, and 51% were male. The median FU was 9.3 (4.3; 20.4) months. A proportion of 33% experienced CVAEs, 91% of them had uncontrolled hypertension, 4.5% acute coronary syndrome, and 4.5% cardiac arrhythmias. Subjects with CVAEs after carfilzomib treatment had significantly higher blood pressure values, left ventricular mass (98 ± 23 vs. 85 ± 17 g/m2, p = 0.01), and pulse wave velocity (8.5 ± 1.7 vs. 7.5 ± 1.6 m/s, p = 0.02) at baseline evaluation compared to the others. Furthermore, baseline uncontrolled blood pressure, left ventricular hypertrophy, and pulse wave velocity ≥ 9 m/s were able to identify patients at higher risk of developing CVAEs during FU. These preliminary findings indicate that blood pressure control, left ventricular mass, and pulse wave velocity may predict CVAEs in MM patients treated with carfilzomib.
Exploring Cellular Stress Response and Chaperones
Authors: Stacchiotti Alessandra
Published: May 2019

Since the pioneering discovery of heat shock proteins in Drosophila by Ferruccio Ritossa in 1960s, a long and exciting journey has been undertaken by molecular biologists and researchers worldwide. Not only lower organisms like worms, yeast, amoeba, and flies but also eukaryotes share common cellular response signals to stressful conditions that can arise from the outside but also from the inside. Moreover, extraordinary interplay between nucleus and subcellular organelles, and between different organelles, like mitochondria and the endoplasmic reticulum called mitochondria-associated endoplasmic reticulum membranes (MAMs), are involved in aging and human diseases like obesity, diabetes, inflammation, neurodegeneration, autoimmune diseases, atherosclerosis, and cancer. Actually, we know that to hit abnormal proteostasis and lipid exchanges in the endoplasmic reticulum is crucial to best guide effective therapies or discover new drugs. Indeed, restoration or impairment of endoplasmic reticulum shape and function lead to cellular homeostasis by autophagy or to final death generally by apoptosis or pyroptosis. This Special Issue collects current valuable articles or reviews on cellular stress research and each contribution opens a new window for further studies and hypothesis. I hope that readers interested in this fascinating topic may be stimulated to know more and more.
Cisplatin-Induced Ototoxicity in Rats Is Driven by RIP3-Dependent Necroptosis
Authors: Choi Mi-Jin , Kang Hyunsook , Lee Yun Yeong , Choo Oak-Sung , Jang Jeong Hun , Park Sung-Hee , Moon Jong-Seok , Choi Seong Jun , Choung Yun-Hoon
Published: May 2019

Cisplatin-induced early-onset ototoxicity is linked to hearing loss. The mechanism by which cisplatin causes ototoxicity remains unclear. The purpose of this study was to identify the involvement of receptor-interacting protein kinase (RIP)3-dependent necroptosis in cisplatin-induced ototoxicity in vitro and in vivo. Sprague–Dawley rats (SD, 8 week) were treated via intraperitoneal (i.p.) injection with cisplatin (16 mg/kg for 1 day), and their hearing thresholds were measured by the auditory brainstem response (ABR) method. Hematoxylin and eosin (H & E) staining, immunohistochemistry, and western blots were performed to determine the effect of cisplatin-induced ototoxicity on cochlear morphology. Inhibitor experiments with necrostatin 1 (Nec-1) and Z-VAD were also performed in HEI-OC1 cell line. H&E stains revealed that the necroptotic changes were increased in the organ of Corti (OC) and spiral ganglion neurons (SGNs). Moreover, immunohistochemistry and western blot analysis showed that cisplatin treatment increased the protein levels of RIP3 in both OCs and SGNs. The treatment of Nec-1, a selective RIP1 inhibitor, resulted in markedly suppression of cisplatin-induced cell death in HEI-OC1 cells, whereas Z-VAD treatment did not change the cisplatin-induced cell death. Our results suggest that RIP3-dependent necroptosis was substantial in cisplatin-induced ototoxicity; inner cochlear regions, the OCs, and SGNs were especially sensitive to necroptosis.
Flexibility Options for Absorption and Distillation to Adapt to Raw Material Supply and Product Demand Uncertainties: A Review
Authors: Riese Julia , Lier Stefan , Paul Sarah , Grünewald Marcus
Published: May 2019

The chemical industry has to deal with increasing uncertainties regarding the boundary conditions of their production processes. On the one hand, uncertainties affect the availability, quality, and prizes of raw material and energy. On the other hand, the demand side is affected by increasing volatilities in product demand and increasing requirements for product variety. These changing boundary conditions lead to higher needs for flexibility in production processes of the chemical industry. Within this article technical solutions for an enhancement of different forms of flexibility are presented for production concepts and apparatus concepts, respectively. The latter focuses on unit operations for the separation of gas–liquid mixtures. This includes a review regarding transformable, modular production processes and a classification of their field of application. Additionally, concepts for named unit operations on different scales are presented and discussed. The presented concepts are also classified with respect to the different types of flexibility.
School-University Links for Evidence-Informed Practice
Authors: Cain Tim
Published: May 2019

A range of studies has identified barriers to evidence-informed practice in schools, many of which recommend school-university links as a means for removing these barriers. In England, public policy also promotes school-university partnerships, which expects these to have benefits for both schools and universities. Secondary analysis of data from five qualitative research projects reveals that school-university links are formed around activities, including postgraduate degrees, research projects and evaluations, Teacher Research projects, research dissemination conferences and seminars, Initial Teacher Education, research-informed Continuous Professional Development (CPD), and bidding for funding. Although, superficially, these activities might seem to enable more and better, evidence-informed practice in schools, school-university links are founded on activities that are declining, those that are short-term, and those that heavily rely on the enthusiasm of a few people. This paper concludes by offering suggestions for improving school-university links, so as to enable research to better inform practice.
Reducing the Superheating of Extraction Stream on Advanced-Ultra Super Critical Power Plants with Regenerative Turbines in South Korea: An Economic Analysis
Authors: Cho Dong-Jin , Lee Eul-Bum , Cho Jae-Min , Alleman Douglas
Published: May 2019

In this study, an advanced-ultra super critical (A-USC) simulation model was developed using the Performance Evaluation of power system efficiencies (PEPSE) software and data collected from a 500 MW ultra-supercritical (USC) coal-fired power plant in South Korea. Using the operational USC and a typical A-USC power plant steam conditions, the model analyzed the impacts of adding an additional feedwater heater (FWH) and reheater to the baseline single reheater (SR) and 8 FWH case. Through the process of introducing reheat and/or regenerative cycles, the authors found: (1) A-USC steam conditions offers an approximate 4% power plant efficiency increase in comparison to the baseline USC steam conditions and; (2) power plant efficiencies increase approximately 1.5% when a 9th FWH and double reheater are added, however; (3) this also results in an approximate 64 °C increase in the superheating of extraction stream. This excessive rise in the superheating of extraction steam was found to cause overall energy loss, reducing the overall efficiency of the power plant. Therefore, it was surmised that if the increase in the superheat degree of extraction steam from the improved steam cycle, which can effectively reduce, the efficiency of the power plant could be further improved. To determine the efficiency variations based on the reduction of the superheat degree of extraction steam, the authors applied a regenerative turbine (RT) to the model. Introducing the RT to the A-USC DR and 9 FWH was found to decrease from the average extraction steam temperature from 221 °C to 108 °C and result in an increase in power plant efficiency of approximately 0.3% to 49.5%. An economic analysis was also performed to assess the fiscal feasibility of adding an RT. Assuming the initial investment to be USD 1409 million, implementing an RT equated to a net present value increase of approximately USD 33 million as compared to that of similar life (30 years of durability) expectancy of A-USC without using an RT. The findings of this study have the potential to improve South Korea’s energy policy, reducing the superheat degree of extraction steam that rises excessively during A-USC steam condition optimization. While this study is focused on South Korea, said findings are also generalizable to worldwide energy policies, serving as an effective method to not only increase system efficiencies, but enhance the economic feasibility as well.
Evaluation of Excavation-Damaged Zone around Underground Tunnels by Theoretical Calculation and Field Test Methods
Authors: Tao Ming , Hong Zhixian , Peng Kang , Sun Pengwei , Cao Mingyu , Du Kun
Published: May 2019

Excavation-damaged zones (EDZs) induced in underground mining and civil engineering potentially threaten tunnel safety and stability, and increase construction and support costs. In this paper, an investigation of the excavation damaged zone (EDZ) around roadways in Fankou lead-zinc mine in Guangzhou, China is performed by applying a seismic velocity method accompanied by SET-PLT-01 nonmetallic ultrasonic detector. Meanwhile, the in situ stress in the mining area was measured based on the stress relief method with the Swedish high-precision LUT system. The results indicate that the stress field is dominated by the maximum horizontal tectonic stress, and the extents of the EDZ on the roof-floor region are greater than that on the sidewall. In addition, both of the in situ stresses and EDZs show an increasing trend with an increase of depth. Analytical solutions of EDZ around circular openings in the brittle rock mass subjected to non-hydrostatic stress fields are presented in terms of the Mohr–Coulomb and generalized Hoek–Brown criteria, and validated by several cases mentioned above. The extents of EDZ solved by closed-form solutions were found to be in a great agreement with those obtained in the field. Finally, a series of parametric studies are conducted to investigate the effects of cohesion (c), friction angle (φ), geological strength index (GSI), mi, uniaxial compressive strength (σc), and disturbance factor (D) on EDZ. It is shown that the effects of c, φ, GSI, and σc are significant; however, more attention should be paid to consider the dynamic disturbances induced by mechanical drilling, blasting, and seismic waves in tunnel excavations or operations.
Tracing the Relationship between Precipitation and River Water in the Northern Carpathians Base on the Evaluation of Water Isotope Data
Authors: Nagavciuc Viorica , Bădăluță Carmen-Andreea , Ionita Monica
Published: May 2019

The aim of this study is to investigate the stable isotope composition of precipitation and river water from the northeastern part of Romania. For this study, we collected monthly samples (for variable periods of time) of precipitation from six stations, and river water from three stations, between March 2012 and December 2017. The precipitation in the area is sourced mainly from the Atlantic Ocean, and secondarily from the Black Sea, local recycling being important especially in summer. We found that the seasonal δ18O in precipitation is in agreement with the seasonal temperature variability, as shown by the significant correlation coefficient between the two variables (r = 0.77), which indicates that the temperature has an important role in the δ18O variability in precipitation water in this region. The local meteoric water line in the northeastern part of Romania is defined by the equation δ2H = 7.80 × δ18O + 7.47, (r2 = 0.99, n = 121). The results presented in this study emphasize that the δ18O (and δ2H) and d-excess variability are strongly influenced by temperature, precipitation and the prevailing large-scale atmospheric circulation.
Relationship between the IL23R SNPs and Crohn’s Disease Susceptibility and Phenotype in the Polish and Bosnian Populations: A Case-Control Study
Authors: Borecki Krzysztof , Zawada Iwona , Salkić Nermin Nusret , Karakiewicz Beata , Adler Grażyna
Published: May 2019

It is suggested that IL-23/IL-17 axis and single nucleotide polymorphisms (SNPs) of IL23R may have crucial role in pathogenesis of Crohn’s disease (CD). Thus, we sought to assess the IL23R SNPs contribution to susceptibility and phenotype of CD. We recruited 117 CD subjects and 117 controls from Poland and 30 CD subjects and 30 controls from Bosnia and Herzegovina (B&H). Two common IL23R SNPs: rs1004819, rs7517847 were genotyped using TaqMan SNP assays. In the Polish population it was found that allele rs1004819: A increases the risk of CD, while allele rs7517847: A is protective against disease development. In Poles the co-carriage of two IL23R risk genotypes was associated with increased risk of CD. A significantly increased risk of CD early onset was observed in Poles carrying at least one rs7517847: G allele. It was also found that IL23R SNPs may be associated with structuring/penetrating CD behavior, as alleles rs1004819: A and rs7517847: G were significantly less frequent in patients without complications, from Poland and B&H, respectively. Allele rs1004819: A was also significantly more frequent in Poles with penetrating CD. These results confirm IL23R SNPs contribution to CD susceptibility in the Polish population and suggest their impact on early age of onset and more severe disease course.
Determinants of Frequent Attendance of Outpatient Physicians: A Longitudinal Analysis Using the German Socio-Economic Panel (GSOEP)
Authors: Hadwiger Moritz , König Hans-Helmut , Hajek André
Published: May 2019

There is a lack of population-based longitudinal studies which investigates the factors leading to frequent attendance of outpatient physicians. Thus, the purpose of this study was to analyze the determinants of frequent attendance using a longitudinal approach. The used dataset comprises seven waves (2002 to 2014; n = 28,574 observations; ranging from 17 to 102 years) from the nationally representative German Socio-Economic Panel (GSOEP). The number of outpatient physician visits in the last three months was used to construct the dependent variable “frequent attendance”. Different cut-offs were used (top 25%; top 10%; top 5%). Variable selection was based on the “behavioral model of health care use” by Andersen. Accordingly, variables were grouped into predisposing, enabling, and need characteristics as well as health behavior, which are possible determinants of frequent attendance. Conditional fixed effects logistic regressions were used. As for predisposing characteristics, regressions showed that getting married and losing one’s job increased the likelihood of frequent attendance. Furthermore, age was negatively associated with the outcome measure. Enabling characteristics were not significantly associated with the outcome measure, except for the onset of the “practice fee”. Decreases in mental and physical health were associated with an increased likelihood of frequent attendance. Findings were robust across different subpopulations. The findings of this study showed that need characteristics are particularly important for the onset of frequent attendance. This might indicate that people begin to use health services frequently when medically indicated.
Polycystins and Mechanotransduction in Human Disease
Authors: Gargalionis Antonios N. , Basdra Efthimia K. , Papavassiliou Athanasios G.
Published: May 2019

Alterations in the process of mechanotransduction have been implicated in the pathogenesis of several diseases such as genetic diseases, osteoporosis, cardiovascular anomalies, and cancer. Several studies over the past twenty years have demonstrated that polycystins (polycystin-1, PC1; and polycystin-2, PC2) respond to changes of extracellular mechanical cues, and mediate pathogenic mechanotransduction and cyst formation in kidney cells. However, recent reports reveal the emergence of polycystins as key proteins that facilitate the transduction of mechano-induced signals in various clinical entities besides polycystic kidney disease, such as cancer, cardiovascular defects, bone loss, and deformations, as well as inflammatory processes like psoriasis. Herewith, we discuss data from recent studies that establish this role with potential clinical utility.
PI3K/AKT/mTOR Signaling Regulates the Virus/Host Cell Crosstalk in HPV-Positive Cervical Cancer Cells
Authors: Bossler Felicitas , Hoppe-Seyler Karin , Hoppe-Seyler Felix
Published: May 2019

Human papillomavirus (HPV)-induced cancers will remain a significant clinical challenge for decades. Thus, the development of novel treatment strategies is urgently required, which should benefit from improving our understanding of the mechanisms of HPV-induced cell transformation. This should also include analyses of hypoxic tumor cells, which represent a major problem for cancer therapy. Recent evidence indicates that the PI3K/AKT/mTOR network plays a key role for the virus/host cell crosstalk in both normoxic and hypoxic HPV-positive cancer cells. In normoxic cells, the efficacy of the senescence induction upon experimental E6/E7 repression depends on active mTORC1 signaling. Under hypoxia, however, HPV-positive cancer cells can evade senescence due to hypoxic impairment of mTORC1 signaling, albeit the cells strongly downregulate E6/E7. Hypoxic repression of E6/E7 is mediated by the AKT kinase, which is activated under hypoxia by its canonical upstream regulators mTORC2 and PI3K. This review highlights our current knowledge about the oxygen-dependent crosstalk of the PI3K/AKT/mTOR signaling circuit with the HPV oncogenes and the phenotypic state of the host cell. Moreover, since the PI3K/AKT/mTOR pathway is considered to be a promising target for anticancer therapy, we discuss clinical implications for the treatment of HPV-positive cervical and head and neck squamous cell carcinomas.
Relationship of Success Rate for Balloon Adhesiolysis with Clinical Outcomes in Chronic Intractable Lumbar Radicular Pain: A Multicenter Prospective Study
Authors: Park Jun-Young , Ji Gyu Yeul , Lee Sang Won , Park Jin Kyu , Ha Dongwon , Park Youngmok , Cho Seong-Sik , Moon Sang Ho , Shin Jin-Woo , Kim Dong Joon , Shin Dong Ah , Choi Seong-Soo
Published: May 2019

Combined balloon decompression and epidural adhesiolysis has been reported to be effective in refractory lumbar spinal stenosis. Many cases of intractable stenosis have symptom-related multiple target sites for interventional treatment. In this situation it may not be possible to perform balloon adhesiolysis, or even only epidural adhesiolysis, for all target sites. Therefore, this multicenter prospective observational study aimed to evaluate the relationship of successful ballooning rate for multiple target sites with clinical outcome. Based on the ballooning success rate of multiple target sites, the patients were divided into three groups: below 50%, 50–85%, and above 85% ballooning. A greater ballooning success rate for multiple target sites provided a more decreased pain intensity and improved functional status in patients with chronic refractory lumbar spinal stenosis, and the improvement was maintained for 6 months. The estimated proportions of successful responders according to a multidimensional approach in the below 50%, 50–85%, and above 85% balloon success groups at 6 months after the procedure were 0.292, 0.468, and 0.507, respectively (p = 0.038). Our study suggests the more successful balloon adhesiolysis procedures for multiple target lesions are performed, the better clinical outcome can be expected at least 6 months after treatment.
Effects of Bi3+ Doping on the Optical and Electric-Induced Light Scattering Performance of PLZT (8.0/69/31) Transparent Ceramics
Authors: Zhu Bin , Zeng Xia , Qiu Pingsun , Ling Liang , Sun Dazhi , Zhao Suchuan , He Xiyun
Published: May 2019

Lanthanum modified lead zirconate titanate (abbreviated as PLZT) Electro-Optical ceramics with various Bi concentration were prepared by a hot-press process. The PLZT ceramic samples all present a single perovskite structure with no second phase detected. Bi3+ ion was considered to mainly enter the A-site of perovskite ABO3 structure of PLZT ceramics by the X-Ray Diffractionanalysis. The maximum permittivity (εm) of the PLZT samples decreased obviously as Bi concentration increased, while their remnant polarization (Pr) decreased slightly. In particular, the PLZT sample with 0.14 wt% Bi doping presented a higher optical transmittance 61.8% (λ = 633 nm), and its electric-induced light scattering performance was found to be improved obviously, the maximum light deduction value of ~47.6% was obtained.
The Microstructure, Electric, Optical and Photovoltaic Properties of BiFeO3 Thin Films Prepared by Low Temperature Sol–Gel Method
Authors: Wang Jiaxi , Luo Li , Han Chunlong , Yun Rui , Tang Xingui , Zhu Yanjuan , Nie Zhaogang , Zhao Weiren , Feng Zhechuan
Published: May 2019

Ferroelectrics have recently attracted attention as a candidate class of materials for use in photovoltaic devices due to their abnormal photovoltaic effect. However, the current reported efficiency is still low. Hence, it is urgent to develop narrow-band gap ferroelectric materials with strong ferroelectricity by low-temperature synthesis. In this paper, the perovskite bismuth ferrite BiFeO3 (BFO) thin films were fabricated on SnO2: F (FTO) substrates by the sol–gel method and they were rapidly annealed at 450, 500 and 550 °C, respectively. The microstructure and the chemical state’s evolution with annealing temperature were investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS), and the relationship between the microstructure and electric, optical and photovoltaic properties were studied. The XRD, SEM and Raman results show that a pure phase BFO film with good crystallinity is obtained at a low annealing temperature of 450 °C. As the annealing temperature increases, the film becomes more uniform and has an improved crystallinity. The XPS results show that the Fe3+/Fe2+ ratio increases and the ratio of oxygen vacancies/lattice oxygen decreases with increasing annealing temperature, which results in the leakage current gradually being reduced. The band gap is reduced from 2.68 to 2.51 eV due to better crystallinity. An enhanced photovoltaic effect is observed in a 550 °C annealed BFO film with a short circuit current of 4.58 mA/cm2 and an open circuit voltage of 0.15 V, respectively.
Four Antioxidant Peptides from Protein Hydrolysate of Red Stingray (Dasyatis akajei) Cartilages: Isolation, Identification, and In Vitro Activity Evaluation
Authors: Pan Xiao-Yang , Wang Yu-Mei , Li Li , Chi Chang-Feng , Wang Bin
Published: May 2019

In the work, water-soluble proteins of red stingray (Dasyatis akajei) cartilages were extracted by guanidine hydrochloride and hydrolyzed using trypsin. Subsequently, four antioxidant peptides (RSHP-A, RSHP-B, RSHP-C, and RSHP-D) were isolated from the water-soluble protein hydrolysate while using ultrafiltration and chromatographic techniques, and the amino acid sequences of RSHP-A, RSHP-B, RSHP-C, and RSHP-D were identified as Val-Pro-Arg (VPR), Ile-Glu-Pro-His (IEPH), Leu-Glu-Glu--Glu-Glu (LEEEE), and Ile-Glu-Glu-Glu-Gln (IEEEQ), with molecular weights of 370.46 Da, 494.55 Da, 647.64 Da, and 646.66 Da, respectively. VPR, IEPH, LEEEE, and IEEEQ exhibited good scavenging activities on the DPPH radical (EC50 values of 4.61, 1.90, 3.69, and 4.01 mg/mL, respectively), hydroxyl radical (EC50 values of 0.77, 0.46, 0.70, and 1.30 mg/mL, respectively), superoxide anion radical (EC50 values of 0.08, 0.17, 0.15, and 0.16 mg/mL, respectively), and ABTS cation radical (EC50 values of 0.15, 0.11, 0.19, and 0.18 mg/mL, respectively). Among the four isolated antioxidant peptides, IEPH showed the strongest reducing power and lipid peroxidation inhibition activity, but LEEEE showed the highest Fe2+-chelating ability. The present results suggested that VPR, IEPH, LEEEE, and IEEEQ might have the possibility of being an antioxidant additive that is used in functional food and pharmaceuticals.
The Metabolomics Society—Current State of the Membership and Future Directions
Authors: Zanetti Krista A. , Hall Robert D. , Griffin Julian L. , Putri Sastia , Salek Reza M. , Styczynski Mark P. , Tugizimana Fidele , van der Hooft Justin J.J.
Published: May 2019

Background: In 2017, the Metabolomics Society conducted a survey among its members to assess the degree of its current success, define opportunities for improving its service to the community and make plans to establish future goals and direction of the Society. Methods: A 32-question online survey was sent via e-mail to all Metabolomics Society members as of 19 June 2017 (n = 644). In addition to the direct e-mails, the link to access the survey was made available through social media. The survey was open until 10 August 2017. Question-specific data were reported using the summary data generated by SurveyMonkey and additional stratified analyses performed using Stata 15. Results: The number of respondents was 394 (61%) with 348 (88%) completing the multiple-choice questions in survey. Metabolomics Society annual meetings, networking and the opportunity to join the global metabolomics community were among the most important benefits expressed by the Metabolomics Society members. Conclusions: The survey collected the first data focusing on membership issues from Society members. The Society should focus on collecting and monitoring of demographic data during the membership registration process; continuing to support the early-career members of the Society; and developing initiatives that focus on member networking to retain and increase Society membership.
The Preparation and Catalytic Properties of Nanoporous Pt/CeO2 Composites with Nanorod Framework Structures
Authors: Wang Haiyang , Duan Dong , Ma Chen , Shi Wenyu , Liang Miaomiao , Wang Liqun , Song Xiaoping , Gao Lumei , Sun Zhanbo
Published: May 2019

Pt/CeO2 catalysts with nanoporous structures were prepared by the facile dealloying of melt-spun Al92−XCe8PtX (X = 0.1; 0.3 and 0.5) ribbons followed by calcination. The phase compositions and structural parameters of the catalysts were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and high-resolution transmission electron microscopy (HRTEM). The specific surface area and pore size distribution were characterized by N2 adsorption–desorption tests. The catalytic properties were evaluated by a three-way catalyst (TWC) measurement system. The results revealed that the dealloyed samples exhibited a nanorod framework structure. The Pt nanoparticles that formed in situ were supported and highly dispersed on the CeO2 nanorod surface and had sizes in the range of 2–5 nm. For the catalyst prepared from the melt-spun Al91.7Ce8Pt0.3 ribbons, the 50% CO conversion temperature (T50) was 91 °C, and total CO could be converted when the temperature was increased to 113 °C. An X-ray photoelectron spectroscopy (XPS) test showed that the Pt0.3/CeO2 sample had a slightly richer oxygen vacancy; and a H2 temperature-programmed reduction (H2-TPR) test demonstrated its superior adsorption ability for reduction gas and high content of active oxygen species. The experiments indicated that the catalytic performance could be retained without any attenuation after 130 h when water and CO2 were present in the reaction gas. The favorable catalytic activities were attributed to the high specific areas and small pore and Pt particle sizes as well as the strong interactions between the CeO2 and Pt nanoparticles. The Pt nanoparticles were embedded in the surface of the CeO2 nanorods, inhibiting growth. Therefore, the catalytic stability and water resistance were excellent.
A Motion-Balanced Sensor Based on the Triboelectricity of Nano-Iron Suspension and Flexible Polymer
Authors: Wang Zhihua , Yang Songyi , Miao Shuang , Shi Qiongfeng , He Tianyiyi , Lee Chengkuo
Published: May 2019

With the development of the Internet of Things and information technology, a large number of inexpensive sensors are needed to monitor the state of the object. A wide variety of sensors with a low cost can be made using the difference in charge attractiveness between flexible polymers and other materials. Compared to the two solid materials, a sensor made of a solid polymer-liquid has a large contact area and low friction. A motion-balanced sensor is presented based on the polytetrafluoroethene pipe and nano-iron suspension. The effect of the concentration and volume of the nano-iron suspension on the output voltage of the sensor is analyzed. The motion-balanced sensor can be used to measure the tilt angle of the object and there is a linear relationship between the output voltage and the tilt angle. A comparison test is performed to a commercial acceleration sensor with PZT-5. The test results show that the frequency characteristics and amplitude characteristics of the motion-balanced sensor are consistent with those of the acceleration sensor. The motion-balanced sensor can be used to determine the state of exercise such as walking, running, etc. The motion-balanced sensor has broad application prospects for monitoring the bridges and power towers balance, stroke patients’ health assessment, etc.
Elemental Distribution and Structural Characterization of GaN/InGaN Core-Shell Single Nanowires by Hard X-ray Synchrotron Nanoprobes
Authors: Secco Eleonora , Mengistu Heruy Taddese , Segura-Ruíz Jaime , Martínez-Criado Gema , García-Cristóbal Alberto , Cantarero Andrés , Foltynski Bartosz , Behmenburg Hannes , Giesen Christoph , Heuken Michael , Garro Núria
Published: May 2019

Improvements in the spatial resolution of synchrotron-based X-ray probes have reached the nano-scale and they, nowadays, constitute a powerful platform for the study of semiconductor nanostructures and nanodevices that provides high sensitivity without destroying the material. Three complementary hard X-ray synchrotron techniques at the nanoscale have been applied to the study of individual nanowires (NWs) containing non-polar GaN/InGaN multi-quantum-wells. The trace elemental sensitivity of X-ray fluorescence allows one to determine the In concentration of the quantum wells and their inhomogeneities along the NW. It is also possible to rule out any contamination from the gold nanoparticle catalyst employed during the NW growth. X-ray diffraction and X-ray absorption near edge-structure probe long- and short-range order, respectively, and lead us to the conclusion that while the GaN core and barriers are fully relaxed, there is an induced strain in InGaN layers corresponding to a perfect lattice matching with the GaN core. The photoluminescence spectrum of non-polar InGaN quntum wells is affected by strain and the inhomogeneous alloy distribution but still exhibits a reasonable 20% relative internal quantum efficiency.
Biomimetic Collagen/Zn2+-Substituted Calcium Phosphate Composite Coatings on Titanium Substrates as Prospective Bioactive Layer for Implants: A Comparative Study Spin Coating vs. MAPLE
Authors: Neacsu Ionela Andreea , Arsenie Laura Vasilica , Trusca Roxana , Ardelean Ioana Lavinia , Mihailescu Natalia , Mihailescu Ion Nicolae , Ristoscu Carmen , Bleotu Coralia , Ficai Anton , Andronescu Ecaterina
Published: May 2019

Synthesis of biomimetic materials for implants and prostheses is a hot topic in nanobiotechnology strategies. Today the major approach of orthopaedic implants in hard tissue engineering is represented by titanium implants. A comparative study of hybrid thin coatings deposition was performed by spin coating and matrix-assisted pulsed laser evaporation (MAPLE) onto titanium substrates. The Collagen-calcium phosphate (Coll-CaPs) combination was selected as the best option to mimic natural bone tissue. To accelerate the mineralization process, Zn2+ ions were inserted by substitution in CaPs. A superior thin film homogeneity was assessed by MAPLE, as shown by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) microscopy. A decrease of P-O and amide absorbance bands was observed as a consequence of different Zn2+ amounts. A variety of structural modifications of the apatite layer are then generated, which influenced the confinement process towards the collagen template. The in-vitro Simulated Body Fluid (SBF) assay demonstrated the ability of Coll/Zn2+-CaPs coatings to stimulate the mineralization process as a result of synergic effects in the collagen-Zn2+ substituted apatite. For both deposition methods, the formation of droplets associated to the growth of CaPs particulates inside the collagen matrix was visualized. This supports the prospective behavior of MAPLE biomimetic coatings to induce mineralization, as an essential step of fast implant integration with vivid tissues.
CdTe0.5S0.5/ZnS Quantum Dots Embedded in a Molecularly Imprinted Polymer for the Selective Optosensing of Dopamine
Authors: Khadem-Abbassi Kiana , Rinnert Hervé , Balan Lavinia , Doumandji Zahra , Joubert Olivier , Masteri-Farahani Majid , Schneider Raphaël
Published: May 2019

This work describes the preparation of molecularly imprinted polymer (MIP)-modified core/shell CdTe0.5S0.5/ZnS quantum dots (QDs). The QDs@MIP particles were used for the selective and sensitive detection of dopamine (DA). Acrylamide, which is able to form hydrogen bonds with DA, and ethylene glycol dimethylacrylate (EGDMA) as cross-linker were used for the preparation of the MIP. Highly cross-linked polymer particles with sizes up to 1 µm containing the dots were obtained after the polymerization. After the removal of the DA template, MIP-modified QDs (QDs@MIP) exhibit a high photoluminescence (PL) with an intensity similar to that of QDs embedded in the nonimprinted polymer (NIP). A linear PL decrease was observed upon addition of DA to QDs@MIP and the PL response was in the linear ranges from 2.63 µM to 26.30 µM with a limit of detection of 6.6 nM. The PL intensity of QDs@MIP was quenched selectively by DA. The QDs@MIP particles developed in this work are easily prepared and of low cost and are therefore of high interest for the sensitive and selective detection of DA in biological samples.
Drug Combinations: Mathematical Modeling and Networking Methods
Authors: Vakil Vahideh , Trappe Wade
Published: May 2019

Treatments consisting of mixtures of pharmacological agents have been shown to have superior effects to treatments involving single compounds. Given the vast amount of possible combinations involving multiple drugs and the restrictions in time and resources required to test all such combinations in vitro, mathematical methods are essential to model the interactive behavior of the drug mixture and the target, ultimately allowing one to better predict the outcome of the combination. In this review, we investigate various mathematical methods that model combination therapies. This survey includes the methods that focus on predicting the outcome of drug combinations with respect to synergism and antagonism, as well as the methods that explore the dynamics of combination therapy and its role in combating drug resistance. This comprehensive investigation of the mathematical methods includes models that employ pharmacodynamics equations, those that rely on signaling and how the underlying chemical networks are affected by the topological structure of the target proteins, and models that are based on stochastic models for evolutionary dynamics. Additionally, this article reviews computational methods including mathematical algorithms, machine learning, and search algorithms that can identify promising combinations of drug compounds. A description of existing data and software resources is provided that can support investigations in drug combination therapies. Finally, the article concludes with a summary of future directions for investigation by the research community.
Acid-Treated Water-Soluble Chitosan Suitable for Microneedle-Assisted Intracutaneous Drug Delivery
Authors: Chandrasekharan Ajeesh , Hwang Young Jun , Seong Keum-Yong , Park Samdae , Kim Sodam , Yang Seung Yun
Published: May 2019

Chitosan has been widely used as a nature-derived polymeric biomaterial due to its high biocompatibility and abundance. However, poor solubility in aqueous solutions of neutral pH and multiple fabrication steps for the molding process limit its application to microneedle technology as a drug delivery carrier. Here, we present a facile method to prepare water-soluble chitosan and its application for sustained transdermal drug delivery. The water-soluble chitosan was prepared by acid hydrolysis using trifluoroacetic acid followed by dialysis in 0.1 M NaCl solutions. We successfully fabricated bullet-shaped microneedle (MN) arrays by the single molding process with neutral aqueous chitosan solutions (pH 6.0). The chitosan MN showed sufficient mechanical properties for skin insertion and, interestingly, exhibited slow dissolving behavior in wet conditions, possibly resulting from a physical crosslinking of chitosan chains. Chitosan MN patches loading rhodamine B, a model hydrophilic drug, showed prolonged release kinetics in the course of the dissolving process for more than 72 h and they were found to be biocompatible to use. Since the water-soluble chitosan can be used for MN fabrication in the mild conditions (neutral pH and 25 °C) required for the loading of bioactive agents such as proteins and achieve a prolonged release, this biocompatible chitosan MN would be suitable for sustained transdermal drug delivery of a diverse range of drugs.
Nanomedicines for the Delivery of Biologics
Authors: Wahlich John , Desai Arpan , Greco Francesca , Hill Kathryn , Jones Arwyn T. , Mrsny Randall J. , Pasut Gianfranco , Perrie Yvonne , Seib F. Philipp , Seymour Leonard W. , Uchegbu Ijeoma F.
Published: May 2019

A special symposium of the Academy of Pharmaceutical Sciences Nanomedicines Focus Group reviewed the current status of the use of nanomedicines for the delivery of biologics drugs. This meeting was particularly timely with the recent approval of the first siRNA-containing product Onpattro™ (patisiran), which is formulated as a lipid nanoparticle for intravenous infusion, and the increasing interest in the use of nanomedicines for the oral delivery of biologics. The challenges in delivering such molecules were discussed with specific emphasis on the delivery both across and into cells. The latest developments in Molecular Envelope Technology® (Nanomerics Ltd, London, UK), liposomal drug delivery (both from an academic and industrial perspective), opportunities offered by the endocytic pathway, delivery using genetically engineered viral vectors (PsiOxus Technologies Ltd, Abingdon, UK), Transint™ technology (Applied Molecular Transport Inc., South San Francisco, CA, USA), which has the potential to deliver a wide range of macromolecules, and AstraZeneca’s initiatives in mRNA delivery were covered with a focus on their uses in difficult to treat diseases, including cancers. Preclinical data were presented for each of the technologies and where sufficiently advanced, plans for clinical studies as well as early clinical data. The meeting covered the work in progress in this exciting area and highlighted some key technologies to look out for in the future.