bluuebunny/crossref_metadata_2025 huggingface dataset:
Effectiveness of Harm Reduction Interventions in Chemsex: A Systematic Review
Authors: Pozo-Herce Pablo Del , Martínez-Sabater Antonio , Sanchez-Palomares Paula , Garcia-Boaventura Paula Cristina , Chover-Sierra Elena , Martínez-Pascual Raquel , Gea-Caballero Vicente , Saus-Ortega Carles , Ballestar-Tarín María Luisa , Karniej Piotr , Baca-García Enrique , Juárez-Vela Raúl
Published: July 2024

The phenomenon of chemsex has emerged as an essential public health issue in recent years. This systematic review aimed to investigate currently available harm reduction strategies and to evaluate the efficacy of the corresponding interventions. Methods: A systematic review of the scientific literature related to harm and risk reduction strategies and the effectiveness of chemsex interventions. Between January 2024 and May 2024, the articles were retrieved from the electronic databases Pubmed, Web of Science, Scopus, PsycInfo, Cochrane, Dialnet, CUIDEN, and SciELO. The review protocol was registered in the PROSPERO database (registration number CRD42024508953). The inclusion criteria were as follows: (I) original studies published in peer-reviewed journals, (II) studies exploring harm reduction interventions for chemsex, and (III) studies reflecting the efficacy of harm reduction interventions for chemsex. Two reviewers independently selected articles by title, abstract, and full paper review and extracted data. Two authors described the selected studies and assessed their methodological quality. Results: The systematic review comprised six scientific papers that met the selection criteria and were obtained from five countries. Although a limited number of studies were included, it was observed that they presented a medium–high methodological quality. Programs evaluated interventions to reduce harm from chemsex, such as a web-based intervention that improved self-efficacy to refuse risky behaviors and accept HIV testing. The studies suggested that peer-led programs can be effective, especially with facilitators who have experienced chemsex dependence. Conclusion: Harm reduction strategies in chemsex are effective and should be promoted by health professionals. Interventions should be accessible, personalized, and non-judgmental to provide appropriate care and support, ensuring a comprehensive and effective public health response.
Arbosana Olive Is Self-Incompatible, but Inter-Compatible with Some Other Low-Vigor Olive Cultivars
Authors: Cuevas Julián , Chiamolera Fernando M. , Pinillos Virginia , Rodríguez Francisco , Salinas Irene , Cabello Diego , Arbeiter Alenka Baruca , Bandelj Dunja , Raboteg Božiković Marina , Vuletin Selak Gabriela
Published: July 2024

Trendy high-density olive fields are often monovarietal orchards, mostly using the cultivar Arbequina. However, Arbequina shows a strong self-incompatibility response, and its yields depend on wind cross-pollination, which is not always available. With the aim of finding suitable self-compatible cultivars that can replace Arbequina, we evaluated pollen–pistil interaction, fruit set and seed paternity in Arbosana under different pollination treatments: self-pollination, open-pollination and three cross-pollination treatments: × Arbequina, × Sikitita and × Koroneiki. All these cultivars are low-vigor cultivars suitable for high-density orchards, making them potential pollinizers for Arbosana. The results show that Arbosana is also self-incompatible with a strong reduction in fruit set due to a lower fertilization level caused by a strong inhibition of pollen tube growth in self-pollinated flowers. Seed-paternity analyses confirmed the self-incompatibility response of Arbosana and suggest that some fruit obtained in bagged shoots under self-pollination were, in fact, a product of cross-fertilization. In conclusion, we recommend against the use of Arbosana in large monovarietal orchards. On the contrary, good results were obtained under cross-pollination with Sikitita, Arbequina and Koroneiki pollen, allowing us to recommend them as pollinizers for Arbosana in appropriate pollination designs. This is the first time Arbosana self-incompatibility has been reported.
Variation in the Photosynthetic Leaf Pigments of Different Basil (Ocimum spp.) Genotypes under Varying Conditions at the Flowering Stage
Authors: Crișan Ioana , Bunea Andrea , Vârban Dan , Cordea Mirela Irina , Horga Vasile , Vînătoru Costel , Stoie Andrei , Vârban Rodica
Published: July 2024

Basil is a culinary herb in high demand all year round, either fresh, dried, or frozen. Field basil crops are commercially predominant, while greenhouse crops can cover the demand for fresh basil during the off-season. The leaf aspect of basil has great importance for the food industry, and a great diversity of genotypes has been created. The aim of this research was to conduct a comparative characterization of 12 basil genotypes (green and purple leaf) that present interest for breeding programs from a national germplasm collection. The study examines the accumulation of photosynthetic pigments in basil cultivated in field versus greenhouse conditions as indicators of plant performance and herb quality from the perspective of ensuring fresh plant material all year round. The experimental median indicated that photosynthetic leaf pigments accumulated in higher concentrations in the field relative to the greenhouse conditions, in descending order: β-carotene, lutein, chlorophyll a, and chlorophyll b. The trend was not as consistent for chlorophyll b, since four out of twelve genotypes presented higher levels in the greenhouse than in the field, while the overall values were not much lower in the greenhouse than the field (16.82%). All genotypes accumulated much higher carotenoid contents in field conditions relative to greenhouse conditions (>200%) and could also provide better nutritional advantages given their demonstrated health benefits. The differences in photosynthetic leaf pigments have both nutritional (the carotenoids), shelf-life, and processing relevance (chlorophylls) and serve as quality markers.
Modeling the Performance Parameters of Pollen Grains of Male Date Palms Using an Artificial Neural Network Based on the Mineral Composition and Morphological Properties of Their Leaves
Authors: Al-Sager Saleh M. , Abdel-Sattar Mahmoud , Al-Obeed Rashid S. , Almady Saad S. , Aboukarima Abdulwahed M.
Published: July 2024

One of the key factors for sustainability in agricultural systems, particularly, for cultivation of date palms, is the identification of the performance parameters of the pollen grains of male date palms (Phoenix dactylifera L.). This study was carried out to predict the performance parameters of pollen grains using an artificial neural network (ANN) model. The morphological features of spathe length, spathe weight, number of pinnae per leaf, leaf length, leaf width, length of the pinna part, pinna length, pinna width, length of the spathe stem, and spathe width, as well as the concentrations of minerals such as Mg, N, K, P, and Ca in their leaves were used as inputs to the ANN model. For this purpose, we collected the required data from nine male date palms grown in Saudi Arabia. The ANN model utilized in this work included an input layer with 15 parameters, a hidden layer of 30 neurons, and an output layer with 8 neurons. The ANN model was trained with 27 patterns. Seven patterns were utilized for testing purposes. The coefficient of determination (R2) obtained between the observed and predicted performance parameters’ values using the testing dataset was 0.902 for the number of strands per spathe, 0.967 for strand length, 0.963 for the number of flowers per strand, 0.941 for the number of flowers per spathe, 0.985 for the weight of pollen grains per spathe, 0.810 for the pollen grains’ viability, 0.936 for the pollen grains’ length, and 0.992 for the pollen grains’ width. The length of the spathe stem had the most critical effect on how the ANN model predicted the values of the dependent variables, i.e., the number of strands per spathe, with a percentage of contribution of 17.66%; the weight of pollen grains per spathe, with 17.85%; the pollen grains’ length, with 19.78%, and the pollen grains’ width, with a percentage of contribution of 30.59%. Spathe weight had the most critical influence on strand length and pollen grains’ viability, with percentages of 26.29% and 14.92%, respectively. Leaf width had the most critical effect on the number of flowers per spathe, with a percentage of 12.55%. The elemental concentration of K in the male date palm leaves had the most critical effect on the number of flowers per strand, with a percentage of 13.98%. It was therefore concluded that using a modeling process with the ANN technique can help estimate the performance parameters of male date palms’ pollen grains for different purposes, such as providing a starting point for mathematical analyses associated with the physiological mechanisms of male date palm. Moreover, the outcomes of this research work can be supportive as a practical tool in this field of study.
Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability
Authors: Pascariu Oana-Elena , Dias Luís Guimarães , Israel-Roming Florentina
Published: July 2024

Phenolic compounds from elderberries (Sambucus nigra L.) have attracted attention due to their potential health benefits. This paper examines different extraction methods used to obtain phenolic compounds from these fruits and the stability of the extracts. Several extraction techniques (extraction with continuous agitation, ultrasound-assisted extraction, microwave-assisted extraction, maceration, and enzyme-assisted extraction) were tested and compared to evaluate the yield and the quality of the extracts. The stability of the extracts with various storage parameters (time and temperature) and processing conditions (concentration and lyophilization) was also investigated. The results showed that ultrasound-assisted extraction (UAE) provided the highest yield of total phenolic compounds (74.89 mg GAE/g), of which 71.23% were represented by total anthocyanins and 62.50% by monomeric anthocyanins, with total flavonoids of 8.11–9.41 mg RUE/g. The analysis of individual phenolic compounds reconfirms the efficiency of UAE, obtaining 0.42–0.09 mg/g gallic acid, 0.59–0.01 mg/g chlorogenic acid, 0.17–0.03 mg/g 4-coumaric acid, and 2.43–0.01 mg/g rutin. The optimization of the extraction conditions led to the conclusion that the best solvent is 45% ethanol (v/v), and the optimal parameters are 40 °C for 40 min. It was also found that the stability of the extracts can be high during long periods of time (even after 180 days). These findings contribute to the understanding of the optimization of extraction processes and storage conditions to obtain extracts rich in phenolic compounds from elderberries, with potential uses in pharmaceutical and food applications.
Integrated Transcriptome and Metabolome to Elucidate the Mechanism of Aluminum-Induced Blue-Turning of Hydrangea Sepals
Authors: Li Wenfang , Lei Penghu , Zhu Tingting , Zhang Huijun , Jiang Hui , Chen Haixia
Published: July 2024

Hydrangea macrophylla is an ornamental plant with varied calyx colors. Interestingly, from red, to purple, to blue, the colors of all Hydrangea macrophylla are formed by unique delphinidin-3-O-glucoside and aluminum ions (Al3+) and 5-O-p-coumaroylquinic acid. The sepals of ‘Blue Mama’ changed from pink to blue, and the contents of delphinidin-3-O-glucoside and aluminum ions increased under 3 g/L aluminum sulfate treatment. However, the mechanism of the effect of aluminum ions on the synthesis and metabolism of anthocyanins in Hydrangea macrophylla is still unclear. In this project, transcriptome sequencing and anthocyanin metabolome analysis were performed on the sepals of ‘Blue Mama’ during flower development at the bud stage (S1), discoloration stage (S2) and full-bloom stage (S3) under aluminum treatment. It was found that delphinidin, delphinidin-3-O-glucoside and delphinidin-3-O-galactoside were the main differential metabolites. The structural genes CHS, F3H, ANS, DFR and BZI in the anthocyanin synthesis pathway were up-regulated with the deepening in sepal color. There was no significant difference between the aluminum treatment and the non-aluminum treatment groups. However, seven transcription factors were up-regulated and expressed to regulate anthocyanin synthesis genes CHS, F3H, BZI and 4CL, promoting the sepals to turn blue. The KEGG enrichment pathway analysis of differentially expressed genes showed that the glutathione metabolism and the ABC transporter pathway were closely related to anthocyanin synthesis and aluminum-ion transport. GST (Hma1.2p1_0158F.1_g069560.gene) may be involved in the vacuolar transport of anthocyanins. The expression of anthocyanin transporter genes ABCC1 (Hma1.2p1_0021F.1_g014400.gene), ABCC2 (Hma1.2p1_0491F.1_g164450.gene) and aluminum transporter gene ALS3 (Hma1.2p1_0111F.1_g053440.gene) were significantly up-regulated in the aluminum treatment group, which may be an important reason for promoting the transport of anthocyanin and aluminum ions to vacuoles and making the sepals blue. These results preliminarily clarified the mechanism of aluminum ion in the synthesis and transport of anthocyanin in Hydrangea macrophylla, laying a foundation for the further study of the formation mechanism of ‘blue complex’ in Hydrangea macrophylla.
Local and Global Public Health and Emissions from Concentrated Animal Feeding Operations in the USA: A Scoping Review
Authors: Pohl Elise , Lee Sang-Ryong
Published: July 2024

Up to 1.6 million tons of waste is produced annually by each of more than 21,000 concentrated animal feeding operations (CAFOs) located in the United States (USA). These operations give rise to externalities, including adverse local and global health impacts from CAFO waste emissions, which can potentially outweigh their economic viability. However, a shortage of evidence synthesis research exclusively on the impacts of USA-based CAFO waste emissions may hinder effective policy development. This scoping review (ScR) study, adhering to the guidelines from the Joanna Briggs Institute, conducted a search in databases including Scopus, Web of Science, PubMed, and Embase in May 2022, resulting in ten publications that met the inclusion criteria. The results suggest possible exposure of CAFO workers to multidrug-resistant Staphylococcus aureus (MDRSA), campylobacteriosis, and cryptosporidiosis. Communities near CAFOs experienced higher rates of adverse health impacts compared to those in non-CAFO areas, with patterns suggesting that proximity may correlate with increased odds of detrimental health effects. Implicit global health threats include methicillin-resistant Staphylococcus aureus (MRSA), MDRSA, campylobacteriosis, tuberculosis, and cryptosporidiosis. These studies provide foundational insights into CAFO proximity, density patterns, and adverse public health effects, indicating a need for evidence-informed environmental health policies to minimize local and global risks.
The Prevalence of and Trend in Drug Use among Adolescents in Mississippi and the United States: Youth Risk Behavior Surveillance System (YRBSS) 2001–2021
Authors: Zhang Zhen , Mitra Amal K. , Schroeder Julie A. , Zhang Lei
Published: July 2024

Mississippi youth are demographically unique compared to those of the nation. The aim of the study was to examine the drug use among adolescents in Mississippi compared to that in the US, which included determining prevalence and trends in drug use as well as drugs on school property and estimating the differences in drug use prevalence by gender and by race. National and Mississippi Youth Risk Behavior Surveillance System (YRBSS) data from 2001 to 2021 were obtained for analysis. Summary statistics, prevalence ratio, and survey Chi-squared tests of independence statistics were generated for the comparison for all students, and by gender and race separately. Trend analysis was conducted using logistic regression combined with joinpoint regression. The six survey questions being studied were the following: have you ever used marijuana, an inhalant, heroin, methamphetamines, or injected drugs, and were you offered, sold, or given an illegal drug on school property during the last 12 months. Survey packages in R were used to account for the complex sampling design of YRBSS data. On the national level, all six drug-related risk behaviors being studied showed a significant decrease from 2001 to 2021. In Mississippi, however, only “ever used marijuana” showed a decrease trend, while three remain unchanged, and two increased. The 2021 YRBSS data show that Mississippi adolescents exhibited a significantly higher prevalence of drug use, and are more likely to be offered, sold or given an illegal drug on school property. This research showed detailed findings on drug use-related issues in Mississippi, which is alarming. This poses an important challenge for public health in Mississippi and sounds an urgent call for drug use intervention among Mississippi adolescents. More concerted actions at the community, school and government level are needed for reducing youth drug use and controlling the drug traffic on school property.
Smartphone Use and Social Media Involvement in Young Adults: Association with Nomophobia, Depression Anxiety Stress Scales (DASS) and Self-Esteem
Authors: Vagka Elissavet , Gnardellis Charalambos , Lagiou Areti , Notara Venetia
Published: July 2024

Excessive smartphone use and dependence on social media give rise to multiple issues that negatively affect the overall well-being of individuals. Nomophobia is characterized as a “digital disease” due to the unlimited use of smartphone devices. The aim of this study is to examine smartphone use and social media involvement in association with nomophobia and psychological traits (i.e., depression, anxiety, stress, and self-esteem) of young adults. A cross-sectional investigation was conducted on 1408 Greek undergraduate students aged 18 to 25 years, using an anonymous self-reported questionnaire. Study data were collected through the Nomophobia Questionnaire (NMP-Q), Depression Anxiety Stress Scale (DASS-21), and Rosenberg Self-Esteem Scale (RSES). Participants’ social media engagement was summarized through four variables: use of social media applications, number of friends, number of followers, and number of messages exchanged daily on social media. High levels of nomophobia were associated with high engagement in social media and, thereby, with a high number of friends. The same applies to participants with high/normal self-esteem compared to those with low. Regarding stress, anxiety and depression were not associated with digital network use, while elevated stress and anxiety seemed to play a negative role in the number of online followers. In addition, high levels of anxiety were correlated to an increased number of messages exchanged through social media applications.
Trends in Complementary Feeding Indicators in Children Aged 6–23 Months According to Participation in a Conditional Cash Transfer Program: Data from the Brazilian Food and Nutrition Surveillance System, 2015–2019
Authors: Andrade-Silva Andreia , Farias Dayana Rodrigues , Carrilho Thais Rangel Bousquet , de Castro Inês Rugani Ribeiro , Kac Gilberto , de Castro Maria Beatriz Trindade
Published: July 2024

Inadequate practices during complementary feeding are associated with malnutrition, especially in children experiencing vulnerable conditions and social inequality. The aim of this study was to evaluate the trends in complementary feeding indicators (CFIs) according to participation in a Brazilian cash transferu program—the Bolsa Família Program (BFP). This was a time-series study with secondary data from 600,138 children assisted from 2015 to 2019 and registered within the Brazilian Food and Nutrition Surveillance System. The CFIs assessed were food introduction, minimum meal frequency and appropriate consistency, minimum dietary diversity, iron-rich food, vitamin A-rich food, ultra-processed food consumption, and zero vegetable or fruit consumption. Prevalence and 95% confidence intervals were calculated for the CFIs according to BFP, the region of residence, and the child’s age. The Prais–Winsten regression method was used to analyze the temporal trend. There was a steady trend for all CFIs of a healthy diet. A decrease in ultra-processed food consumption for both BFP (−10.02%) and non-BFP children (−9.34%) was observed over the years. Children residing in the North and Northeast regions and those enrolled in the BFP were more distant from the recommended feeding practices when compared to the other regions and non-BFP children. The results highlight the relevance of nutritional surveillance and the need to improve food and nutrition public policies for children aged 6–23 months, particularly for those experiencing greater social vulnerability.
Practical Considerations of Workplace Wellbeing Management under Post-Pandemic Work-from-Home Conditions
Authors: Cheung Victor K. L.
Published: July 2024

As a natural experiment or “stress test” on the rapidly shifting work environment from office to home during and after the COVID-19 pandemic, staff wellbeing has been considered as the most critical issue in organizational change management. Following an overview of the relevant literature and recent official statistics, this essay aims to (i) address the major considerations and challenges in light of the transformation and re-design of the mode of work in the new normal and (ii) inform practical decisions for overall staff wellbeing under post-pandemic work-from-home (WFH) conditions with recommendations. For the sake of both staff healthiness and safety, as well as organizational competitiveness, senior management should take reasonable steps to enhance occupational safety in their WFH policy in line with practical recommendations on five areas, namely, (i) ergonomics, (ii) stress and anxiety management, (iii) workplace boundaries, (iv) work–family conflicts, and (v) other factors regarding a negative work atmosphere (e.g., loneliness attack, burnout, and workplace violence) particularly on virtual platforms. With the suggested evidence-based practices on WFH initiatives, senior management could make a difference in optimizing the overall workplace wellbeing of staff after the pandemic.
The Undeniable Potential of Thermophiles in Industrial Processes
Authors: Gallo Giovanni , Imbimbo Paola , Aulitto Martina
Published: July 2024

Extremophilic microorganisms play a key role in understanding how life on Earth originated and evolved over centuries. Their ability to thrive in harsh environments relies on a plethora of mechanisms developed to survive at extreme temperatures, pressures, salinity, and pH values. From a biotechnological point of view, thermophiles are considered a robust tool for synthetic biology as well as a reliable starting material for the development of sustainable bioprocesses. This review discusses the current progress in the biomanufacturing of high-added bioproducts from thermophilic microorganisms and their industrial applications.
Saturation Mutagenesis and Molecular Modeling: The Impact of Methionine 182 Substitutions on the Stability of β-Lactamase TEM-1
Authors: Grigorenko Vitaly G. , Krivitskaya Alexandra V. , Khrenova Maria G. , Rubtsova Maya Yu. , Presnova Galina V. , Andreeva Irina P. , Serova Oxana V. , Egorov Alexey M.
Published: July 2024

Serine β-lactamase TEM-1 is the first β-lactamase discovered and is still common in Gram-negative pathogens resistant to β-lactam antibiotics. It hydrolyzes penicillins and cephalosporins of early generations. Some of the emerging TEM-1 variants with one or several amino acid substitutions have even broader substrate specificity and resistance to known covalent inhibitors. Key amino acid substitutions affect catalytic properties of the enzyme, and secondary mutations accompany them. The occurrence of the secondary mutation M182T, called a “global suppressor”, has almost doubled over the last decade. Therefore, we performed saturating mutagenesis at position 182 of TEM-1 to determine the influence of this single amino acid substitution on the catalytic properties, thermal stability, and ability for thermoreactivation. Steady-state parameters for penicillin, cephalothin, and ceftazidime are similar for all TEM-1 M182X variants, whereas melting temperature and ability to reactivate after incubation at a higher temperature vary significantly. The effects are multidirectional and depend on the particular amino acid at position 182. The M182E variant of β-lactamase TEM-1 demonstrates the highest residual enzymatic activity, which is 1.5 times higher than for the wild-type enzyme. The 3D structure of the side chain of residue 182 is of particular importance as observed from the comparison of the M182I and M182L variants of TEM-1. Both of these amino acid residues have hydrophobic side chains of similar size, but their residual activity differs by three-fold. Molecular dynamic simulations add a mechanistic explanation for this phenomenon. The important structural element is the V159-R65-E177 triad that exists due to both electrostatic and hydrophobic interactions. Amino acid substitutions that disturb this triad lead to a decrease in the ability of the β-lactamase to be reactivated.
Beneficial Effects of Probiotic Lactobacillus paraplantarum BGCG11 on Pancreatic and Duodenum Function in Diabetic Rats
Authors: Mihailović Mirjana , Soković Bajić Svetlana , Arambašić Jovanović Jelena , Brdarić Emilija , Dinić Svetlana , Grdović Nevena , Uskoković Aleksandra , Rajić Jovana , Đorđević Marija , Tolinački Maja , Golić Nataša , Živković Milica , Vidaković Melita
Published: July 2024

Diabetes mellitus, as a chronic metabolic disorder, significantly impacts the pancreas and among other organs, affects duodenal function. Emerging evidence suggests that probiotics can exert beneficial effects on gut health and metabolism. In our previous research, we evaluated the probiotic Lactobacillus paraplantarum BGCG11 primarily for its protective properties against diabetic rats’ damaged liver and kidneys. In this work, we further examined the effects of probiotic strain BGCG11 on the function of the duodenum and pancreas in diabetic rats. We explored the potential mechanisms underlying the probiotic’s effects, focusing on general indicators of diabetes, the architecture and morphology of pancreatic islets, duodenal integrity (measuring the transfer of fluid and serum zonulin level), and the modulation of gut microbiota composition. Our findings reveal the protective and regulatory roles of L. paraplantarum BGCG11 in mitigating diabetes-induced pancreatic and duodenal dysfunction regardless of its application time (pre- or post-treatment), highlighting its therapeutic potential in managing diabetes-related gastrointestinal complications.
Curcumin and Gut Microbiota: A Narrative Overview with Focus on Glycemic Control
Authors: Servida Simona , Piontini Alessandra , Gori Francesca , Tomaino Laura , Moroncini Gianluca , De Gennaro Colonna Vito , La Vecchia Carlo , Vigna Luisella
Published: July 2024

Turmeric is a spice widely used in China, Southeast Asia, and in traditional Ayurvedic medicine. Its safety profile and efficacy as an antioxidant, anti-inflammatory, antimicrobial, antitumor, antidiabetic, and anti-obesity agent have led to extensive research into its potential role in preventing and treating metabolic diseases. The active compound in turmeric is curcumin, which exhibits low systemic bioavailability after oral administration. However, it is detectable in the gut, where it bidirectionally interacts with the gut microbiota (GM), which plays a crucial role in maintaining host health. The favorable effects of curcumin, particularly its hypoglycemic properties, are linked to alteration in intestinal dysbiosis observed in type 2 diabetes mellitus and metabolic syndrome patients. Restoration of the eubiotic GM may contribute to glycemic homeostasis. Preclinical and clinical studies have demonstrated the involvement of the GM in the regulation of glucose and lipid metabolism. Although the underlying mechanism remains incompletely understood, intestinal dysbiosis is associated with insulin resistance, hyperglycemia, and low-grade inflammation. In the present overview, we summarize the biological properties of curcumin, focusing on its link with GM and, therefore, on its potential role in metabolic diseases.
Acute Effects of Dietary Protein Consumption on the Postprandial Metabolic Response, Amino Acid Levels and Circulating MicroRNAs in Patients with Obesity and Insulin Resistance
Authors: Hernández-Gómez Karla G. , Velázquez-Villegas Laura A. , Granados-Portillo Omar , Avila-Nava Azalia , González-Salazar Luis E. , Serralde-Zúñiga Aurora E. , Palacios-González Berenice , Pichardo-Ontiveros Edgar , Guizar-Heredia Rocio , López-Barradas Adriana M. , Sánchez-Tapia Mónica , Larios-Serrato Violeta , Olin-Sandoval Viridiana , Díaz-Villaseñor Andrea , Medina-Vera Isabel , Noriega Lilia G. , Alemán-Escondrillas Gabriela , Ortiz-Ortega Victor M. , Torres Nimbe , Tovar Armando R. , Guevara-Cruz Martha
Published: July 2024

The post-nutritional intervention modulation of miRNA expression has been previously investigated; however, post-acute dietary-ingestion-related miRNA expression dynamics in individuals with obesity and insulin resistance (IR) are unknown. We aimed to determine the acute effects of protein ingestion from different dietary sources on the postprandial metabolic response, amino acid levels, and circulating miRNA expression in adults with obesity and IR. This clinical trial included adults with obesity and IR who consumed (1) animal-source protein (AP; calcium caseinate) or (2) vegetable-source protein (VP; soy protein isolate). Glycaemic, insulinaemic, and glucagon responses, amino acid levels, and exosomal microRNAs isolated from plasma were analysed. Post-AP ingestion, the area under the curve (AUC) of insulin (p = 0.04) and the plasma concentrations of branched-chain (p = 0.007) and gluconeogenic (p = 0.01) amino acids increased. The effects of different types of proteins on the concentration of miRNAs were evaluated by measuring their plasma circulating levels. Compared with the baseline, the AP group presented increased circulating levels of miR-27a-3p, miR-29b-3p, and miR-122-5p (p < 0.05). Subsequent analysis over time at 0, 30, and 60 min revealed the same pattern and differences between treatments. We demonstrated that a single dose of dietary protein has acute effects on hormonal and metabolic regulation and increases exosomal miRNA expression in individuals with obesity and IR.
Itch and Pain Behaviors in Irritant Contact Dermatitis Produced by Sodium Lauryl Sulfate in Mice
Authors: Malewicz-Oeck Nathalie M. , Zhang Zhe , Shimada Steven G. , LaMotte Robert H.
Published: July 2024

Irritant contact dermatitis (ICD) is a nonspecific skin inflammation caused by irritants, leading to itch and pain. We tested whether differential responses to histamine-dependent and -independent pruritogens can be evoked in ICD induced by sodium lauryl sulfate (SLS). An ICD mouse model was established with 5% SLS in acetone versus a vehicle topically applied for 24 h to the cheek. Site-directed itch- and pain-like behaviors, occurring spontaneously and in response to mechanical, thermal, and chemical stimuli (histamine, ß-alanine, BAM8-22, and bradykinin) applied to the cheek, were recorded before (day 0) and after irritant removal (days 1, 2, 3, and 4). Skin inflammation was assessed through visual scoring, ultrasound, and measurements of skin thickness. SLS-treated mice exhibited hyperalgesia-like behavior in response to mechanical and heat stimuli on day 1 compared to the controls. SLS mice exhibited more spontaneous wipes (pain) but not scratching bouts (itch) on day 1. Pruritogen injections caused more scratching but not wiping in SLS-treated mice compared to the controls. Only bradykinin increased wiping behavior compared to saline. SLS-treated mice developed noticeable erythema, scaling, and increased skin thickness on days 1 and 2. SLS induced cutaneous inflammation and behavioral signs of spontaneous pain and itching, hyperalgesia to mechanical and heat stimuli and a chemical algogen, and enhanced itch response to pruritogens. These sensory reactions preceded the inflammation peak and lasted up to two days.
Low-Temperature Regulates the Cell Structure and Chlorophyll in Addition to Cellulose Metabolism of Postharvest Red Toona sinensis Buds across Different Seasons
Authors: Zhao Qian , Wang Fu , Wang Yifei , Zhong Xiulai , Zhu Shunhua , Zhang Xinqi , Li Shuyao , Lei Xiujuan , Zang Zhenyuan , Tan Guofei , Zhang Jian
Published: July 2024

Postharvest fibrosis and greening of Toona sinensis buds significantly affect their quality during storage. This study aimed to clarify the effects of low-temperature storage on postharvest red TSB quality harvested in different seasons. Red TSB samples were collected from Guizhou province, China, 21 days after the beginning of spring (Lichun), summer (Lixia), and autumn (Liqiu), and stored at 4 °C in dark conditions. We compared and analyzed the appearance, microstructure, chlorophyll and cellulose content, and expression levels of related genes across different seasons. The results indicated that TSB harvested in spring had a bright, purple-red color, whereas those harvested in summer and autumn were green. All samples lost water and darkened after 1 day of storage. Severe greening occurred in spring-harvested TSB within 3 days, a phenomenon not observed in summer and autumn samples. Microstructural analysis revealed that the cells in the palisade and spongy tissues of spring and autumn TSB settled closely during storage, while summer TSB cells remained loosely aligned. Xylem cells were smallest in spring-harvested TSB and largest in autumn. Prolonged storage led to thickening of the secondary cell walls and pith cell autolysis in the petioles, enlarging the cavity area. Chlorophyll content was higher in leaves than in petioles, while cellulose content was lower in petioles across all seasons. Both chlorophyll and cellulose content increased with storage time. Gene expression analysis showed season-dependent variations and significant increases in the expression of over half of the chlorophyll-related and cellulose-related genes during refrigeration, correlating with the observed changes in chlorophyll and cellulose content. This research provides valuable insights for improving postharvest storage and freshness preservation strategies for red TSB across different seasons.
Effects of Age and MPTP-Induced Parkinson’s Disease on the Expression of Genes Associated with the Regulation of the Sleep–Wake Cycle in Mice
Authors: Semenova Ekaterina I. , Rudenok Margarita M. , Rybolovlev Ivan N. , Shulskaya Marina V. , Lukashevich Maria V. , Partevian Suzanna A. , Budko Alexander I. , Nesterov Maxim S. , Abaimov Denis A. , Slominsky Petr A. , Shadrina Maria I. , Alieva Anelya Kh.
Published: July 2024

Parkinson’s disease (PD) is characterized by a long prodromal period, during which patients often have sleep disturbances. The histaminergic system and circadian rhythms play an important role in the regulation of the sleep–wake cycle. Changes in the functioning of these systems may be involved in the pathogenesis of early stages of PD and may be age-dependent. Here, we have analyzed changes in the expression of genes associated with the regulation of the sleep–wake cycle (Hnmt, Hrh1, Hrh3, Per1, Per2, and Chrm3) in the substantia nigra (SN) and striatum of normal male mice of different ages, as well as in young and adult male mice with an MPTP-induced model of the early symptomatic stage (ESS) of PD. Age-dependent expression analysis in normal mouse brain tissue revealed changes in Hrh3, Per1, Per2, and Chrm3 genes in adult mice relative to young mice. When gene expression was examined in mice with the MPTP-induced model of the ESS of PD, changes in the expression of all studied genes were found only in the SN of adult mice with the ESS model of PD. These data suggest that age is a significant factor influencing changes in the expression of genes associated with sleep–wake cycle regulation in the development of PD.
The Utility of Lipidomic Analysis in Colorectal Cancer Diagnosis and Prognosis—A Systematic Review of Recent Literature
Authors: Klekowski Jakub , Chabowski Mariusz , Krzystek-Korpacka Małgorzata , Fleszar Mariusz
Published: July 2024

Colorectal cancer (CRC) is among the most prevalent and lethal malignancies. Lipidomic investigations have revealed numerous disruptions in lipid profiles across various cancers. Studies on CRC exhibit potential for identifying novel diagnostic or prognostic indicators through lipidomic signatures. This review examines recent literature regarding lipidomic markers for CRC. PubMed database was searched for eligible articles concerning lipidomic biomarkers of CRC. After selection, 36 articles were included in the review. Several studies endeavor to establish sets of lipid biomarkers that demonstrate promising potential to diagnose CRC based on blood samples. Phosphatidylcholine, phosphatidylethanolamine, ceramides, and triacylglycerols (TAGs) appear to offer the highest diagnostic accuracy. In tissues, lysophospholipids, ceramides, and TAGs were among the most altered lipids, while unsaturated fatty acids also emerged as potential biomarkers. In-depth analysis requires both cell culture and animal studies. CRC involves multiple lipid metabolism alterations. Although numerous lipid species have been suggested as potential diagnostic markers, the establishment of standardized methods and the conduct of large-scale studies are necessary to facilitate their clinical application.
Pervaporation Dehydration Mechanism and Performance of High-Aluminum ZSM-5 Zeolite Membranes for Organic Solvents
Authors: Wang Qing , Qian Cheng , Guo Changxu , Xu Nong , Liu Qiao , Wang Bin , Fan Long , Hu Kunhong
Published: July 2024

Membrane-based pervaporation (PV) for organic solvent dehydration is of great significance in the chemical and petrochemical industries. In this work, high-aluminum ZSM-5 zeolite membranes were synthesized by a fluoride-assisted secondary growth on α-alumina tubular supports using mordenite framework inverted (MFI) nanoseeds (~110 nm) and a template-free synthesis solution with a low Si/Al ratio of 10. Characterization by XRD, EDX, and SEM revealed that the prepared membrane was a pure-phase ZSM-5 zeolite membrane with a Si/Al ratio of 3.8 and a thickness of 2.8 µm. Subsequently, two categories of PV performance parameters (i.e., flux versus separation factor and permeance versus selectivity) were used to systematically examine the effects of operating conditions on the PV dehydration performance of different organic solvents (methanol, ethanol, n-propanol, and isopropanol), and their PV mechanisms were explored. Employing permeance and selectivity effectively disentangles the influence of operating conditions on PV performance, thereby elucidating the inherent contribution of membranes to separation performance. The results show that the mass transfer during PV dehydration of organic solvents was mainly dominated by the adsorption–diffusion mechanism. Furthermore, the diffusion of highly polar water and methanol molecules within membrane pores had a strong mutual slowing-down effect, resulting in significantly lower permeance than other binary systems. However, the mass transfer process for water/low-polar organic solvent (ethanol, n-propanol, and isopropanol) mixtures was mainly controlled by competitive adsorption caused by affinity differences. In addition, the high-aluminum ZSM-5 zeolite membrane exhibited superior PV dehydration performance for water/isopropanol mixtures.
What Not to Overlook in the Management of Patients with Type 2 Diabetes Mellitus: The Nephrological and Hepatological Perspectives
Authors: Alfieri Carlo Maria , Molinari Paolo , Cinque Felice , Vettoretti Simone , Cespiati Annalisa , Bignamini Daniela , Nardelli Luca , Fracanzani Anna Ludovica , Castellano Giuseppe , Lombardi Rosa
Published: July 2024

Diabetes mellitus (DM) significantly impacts renal and hepatic function, necessitating comprehensive understanding and management strategies. Renal involvement, namely diabetic kidney disease (DKD), presents a global challenge, with increasing prevalence paralleling DM rates. Lifestyle modifications and pharmacotherapy targeting hypertension and glycemic control have pivotal roles in DKD management. Concurrently, hepatic involvement in DM, characterized by metabolic dysfunction-associated steatotic liver disease (MASLD), presents a bidirectional relationship. DM exacerbates MASLD progression, while MASLD predisposes to DM development and worsens glycemic control. Screening for MASLD in DM patients is of high importance, utilizing non-invasive methods like ultrasound and fibrosis scores. Lifestyle modifications, such as weight loss and a Mediterranean diet, mitigate MASLD progression. Promising pharmacotherapies, like SGLT2 inhibitors and GLP-1 agonists, demonstrate efficacy in both DM and MASLD management. Special populations, such as diabetic individuals undergoing hemodialysis or kidney transplant recipients, demand special care due to unique clinical features. Similarly, DM exacerbates complications in MASLD patients, elevating the risks of hepatic decompensation and hepatocellular carcinoma. Recognizing the interconnectedness of DM, renal, and hepatic diseases underscores the need for multidisciplinary approaches for optimal patient outcomes. The present review aims to present the main characteristics and crucial points not to be overlooked regarding the renal and hepatic involvement in DM patients focusing on the inter-relationships between the renal and the hepatic involvements.
Recent Progress in the Research on RNA-Binding Proteins in Bone Development and Diseases
Authors: Farooq Hafiz Muhammad Umer , Yang Lihuizi , Cao Mengru , Chen Zhihao , Qian Airong , Dang Kai
Published: July 2024

RNA-binding proteins (RBPs), which regulate gene expression through post-transcriptional modifications of RNAs, play a role in diverse biological processes that include bone cell development and bone tissue formation. RBP dysregulation may result in aberrant bone homeostasis and contribute to various bone diseases. The function of RBPs in bone physiology and pathophysiology and the underlying molecular mechanisms have been extensively studied in recent years. This article provides a review of such studies, highlighting the potential of RBPs as pivotal targets for therapeutic intervention.
Autotaxin–Lysophosphatidate Axis: Promoter of Cancer Development and Possible Therapeutic Implications
Authors: Laface Carmelo , Ricci Angela Dalia , Vallarelli Simona , Ostuni Carmela , Rizzo Alessandro , Ambrogio Francesca , Centonze Matteo , Schirizzi Annalisa , De Leonardis Giampiero , D’Alessandro Rosalba , Lotesoriere Claudio , Giannelli Gianluigi
Published: July 2024

Autotaxin (ATX) is a member of the ectonucleotide pyrophosphate/phosphodiesterase (ENPP) family; it is encoded by the ENPP2 gene. ATX is a secreted glycoprotein and catalyzes the hydrolysis of lysophosphatidylcholine to lysophosphatidic acid (LPA). LPA is responsible for the transduction of various signal pathways through the interaction with at least six G protein-coupled receptors, LPA Receptors 1 to 6 (LPAR1–6). The ATX–LPA axis is involved in various physiological and pathological processes, such as angiogenesis, embryonic development, inflammation, fibrosis, and obesity. However, significant research also reported its connection to carcinogenesis, immune escape, metastasis, tumor microenvironment, cancer stem cells, and therapeutic resistance. Moreover, several studies suggested ATX and LPA as relevant biomarkers and/or therapeutic targets. In this review of the literature, we aimed to deepen knowledge about the role of the ATX–LPA axis as a promoter of cancer development, progression and invasion, and therapeutic resistance. Finally, we explored its potential application as a prognostic/predictive biomarker and therapeutic target for tumor treatment.
PKR Mediates the Mitochondrial Unfolded Protein Response through Double-Stranded RNA Accumulation under Mitochondrial Stress
Authors: Kusuma Fedho , Park Soyoung , Nguyen Kim Anh , Elvira Rosalie , Lee Duckgue , Han Jaeseok
Published: July 2024

Mitochondrial stress, resulting from dysfunction and proteostasis disturbances, triggers the mitochondrial unfolded protein response (UPRMT), which activates gene encoding chaperones and proteases to restore mitochondrial function. Although ATFS-1 mediates mitochondrial stress UPRMT induction in C. elegans, the mechanisms relaying mitochondrial stress signals to the nucleus in mammals remain poorly defined. Here, we explored the role of protein kinase R (PKR), an eIF2α kinase activated by double-stranded RNAs (dsRNAs), in mitochondrial stress signaling. We found that UPRMT does not occur in cells lacking PKR, indicating its crucial role in this process. Mechanistically, we observed that dsRNAs accumulate within mitochondria under stress conditions, along with unprocessed mitochondrial transcripts. Furthermore, we demonstrated that accumulated mitochondrial dsRNAs in mouse embryonic fibroblasts (MEFs) deficient in the Bax/Bak channels are not released into the cytosol and do not induce the UPRMT upon mitochondrial stress, suggesting a potential role of the Bax/Bak channels in mediating the mitochondrial stress response. These discoveries enhance our understanding of how cells maintain mitochondrial integrity, respond to mitochondrial dysfunction, and communicate stress signals to the nucleus through retrograde signaling. This knowledge provides valuable insights into prospective therapeutic targets for diseases associated with mitochondrial stress.
Exploring Fecal Microbiota Transplantation for Modulating Inflammation in Parkinson’s Disease: A Review of Inflammatory Markers and Potential Effects
Authors: Sadowski Karol , Zając Weronika , Milanowski Łukasz , Koziorowski Dariusz , Figura Monika
Published: July 2024

Parkinson’s disease (PD) is a complex neurodegenerative disorder characterized by numerous motor and non-motor symptoms. Recent data highlight a potential interplay between the gut microbiota and the pathophysiology of PD. The degeneration of dopaminergic neurons in PD leads to motor symptoms (tremor, rigidity, and bradykinesia), with antecedent gastrointestinal manifestations, most notably constipation. Consequently, the gut emerges as a plausible modulator in the neurodegenerative progression of PD. Key molecular changes in PD are discussed in the context of the gut–brain axis. Evidence suggests that the alterations in the gut microbiota composition may contribute to gastroenteric inflammation and influence PD symptoms. Disturbances in the levels of inflammatory markers, including tumor necrosis factor-α (TNF α), interleukin -1β (IL-1β), and interleukin-6 (IL-6), have been observed in PD patients. These implicate the involvement of systemic inflammation in disease pathology. Fecal microbiota transplantation emerges as a potential therapeutic strategy for PD. It may mitigate inflammation by restoring gut homeostasis. Preclinical studies in animal models and initial clinical trials have shown promising results. Overall, understanding the interplay between inflammation, the gut microbiota, and PD pathology provides valuable insights into potential therapeutic interventions. This review presents recent data about the bidirectional communication between the gut microbiome and the brain in PD, specifically focusing on the involvement of inflammatory biomarkers.