bluuebunny/crossref_metadata_2025 huggingface dataset:
Two Stage Implicit Method on Hexagonal Grids for Approximating the First Derivatives of the Solution to the Heat Equation
Authors: Buranay Suzan Cival , Matan Ahmed Hersi , Arshad Nouman
Published: February 2021

The first type of boundary value problem for the heat equation on a rectangle is considered. We propose a two stage implicit method for the approximation of the first order derivatives of the solution with respect to the spatial variables. To approximate the solution at the first stage, the unconditionally stable two layer implicit method on hexagonal grids given by Buranay and Arshad in 2020 is used which converges with Oh2+τ2 of accuracy on the grids. Here, h and 32h are the step sizes in space variables x1 and x2, respectively and τ is the step size in time. At the second stage, we propose special difference boundary value problems on hexagonal grids for the approximation of first derivatives with respect to spatial variables of which the boundary conditions are defined by using the obtained solution from the first stage. It is proved that the given schemes in the difference problems are unconditionally stable. Further, for r=ωτh2≤37, uniform convergence of the solution of the constructed special difference boundary value problems to the corresponding exact derivatives on hexagonal grids with order Oh2+τ2 is shown. Finally, the method is applied on a test problem and the numerical results are presented through tables and figures.
Quantifying Fetal Reprogramming for Biomarker Development in the Era of High-Throughput Sequencing
Authors: Chou Fu-Sheng , Newton Krystel , Wang Pei-Shan
Published: February 2021

Gestational hypertensive disorders continue to threaten the well-being of pregnant women and their offspring. The only current definitive treatment for gestational hypertensive disorders is delivery of the fetus. The optimal timing of delivery remains controversial. Currently, the available clinical tools do not allow for assessment of fetal stress in its early stages. Placental insufficiency and fetal growth restriction secondary to gestational hypertensive disorders have been shown to have long-term impacts on offspring health even into their adulthood, becoming one of the major focuses of research in the field of developmental origins of health and disease. Fetal reprogramming was introduced to describe the long-lasting effects of the toxic intrauterine environment on the growing fetus. With the advent of high-throughput sequencing, there have been major advances in research attempting to quantify fetal reprogramming. Moreover, genes that are found to be differentially expressed as a result of fetal reprogramming show promise in the development of transcriptional biomarkers for clinical use in detecting fetal response to placental insufficiency. In this review, we will review key pathophysiology in the development of placental insufficiency, existing literature on high-throughput sequencing in the study of fetal reprogramming, and considerations regarding research design from our own experience.
Current Knowledge on Genomic Profiling of Upper Tract Urothelial Carcinoma
Authors: De Lorenzis Elisa , Albo Giancarlo , Longo Fabrizio , Bebi Carolina , Boeri Luca , Montanari Emanuele
Published: February 2021

Recent research in next-generation sequencing characterized the genomic landscape of urothelial cancer. However, the majority of the studies focused on bladder cancer (BC). Upper urinary tract urothelial carcinomas (UTUC) and BC share some histological characteristics, but, considering the differences in terms of embryologic precursors, epidemiology, genetics, medical and surgical management and response to therapy, UTUC and BC should be considered as two distinct diseases. Our objective is to analyze through a literature search the latest updates and the current knowledge about the genomics of UTUC. We also evaluate genetic differences between BC and UTUC and the potential implications for systemic therapy. Molecular subtyping and variant histology and their correlation with response to chemotherapy were also explored. In summary, the most frequent genomic variations in UTUC included FGFR3, chromatin remodeling genes, TP53/MDM2 and other tumor suppressors/oncogenes. The genomics of UTUC, integrated with clinical data, could drive the selection of patients who could benefit from targeted therapy or off-label treatment. Routine implementation of tumor genomic characterization in UTUC patients should therefore be contemplated and evaluated prospectively.
Network Analysis of Gene Transcriptions of Arabidopsis thaliana in Spaceflight Microgravity
Authors: Manian Vidya , Orozco Jairo , Gangapuram Harshini , Janwa Heeralal , Agrinsoni Carlos
Published: February 2021

The transcriptomic datasets of the plant model organism Arabidopsis thaliana grown in the International Space Station provided by GeneLab have been mined to isolate the impact of spaceflight microgravity on gene expressions related to root growth. A set of computational tools is used to identify the hub genes that respond differently in spaceflight with controlled lighting compared to on the ground. These computational tools based on graph-theoretic approaches are used to infer gene regulatory networks from the transcriptomic datasets. The three main algorithms used for network analyses are Least Absolute Shrinkage and Selection Operator (LASSO), Pearson correlation, and the Hyperlink-Induced Topic Search (HITS) algorithm. Graph-based spectral analyses reveal distinct properties of the spaceflight microgravity networks for the Wassilewskija (WS), Columbia (Col)-0, and mutant phytochromeD (phyD) ecotypes. The set of hub genes that are significantly altered in spaceflight microgravity are mainly involved in cell wall synthesis, protein transport, response to auxin, stress responses, and catabolic processes. Network analysis highlights five important root growth-regulating hub genes that have the highest outdegree distribution in spaceflight microgravity networks. These concerned genes coding for proteins are identified from the Gene Regulatory Networks (GRNs) corresponding to spaceflight total light environment. Furthermore, network analysis uncovers genes that encode nucleotide-diphospho-sugar interconversion enzymes that have higher transcriptional regulation in spaceflight microgravity and are involved in cell wall biosynthesis.
The Independent Domestication of Timopheev’s Wheat: Insights from Haplotype Analysis of the Brittle rachis 1 (BTR1-A) Gene
Authors: Nave Moran , Taş Mihriban , Raupp John , Tiwari Vijay K. , Ozkan Hakan , Poland Jesse , Hale Iago , Komatsuda Takao , Distelfeld Assaf
Published: February 2021

Triticum turgidum and T. timopheevii are two tetraploid wheat species sharing T. urartu as a common ancestor, and domesticated accessions from both of these allopolyploids exhibit nonbrittle rachis (i.e., nonshattering spikes). We previously described the loss-of-function mutations in the Brittle Rachis 1 genes BTR1-A and BTR1-B in the A and B subgenomes, respectively, that are responsible for this most visible domestication trait in T. turgidum. Resequencing of a large panel of wild and domesticated T. turgidum accessions subsequently led to the identification of the two progenitor haplotypes of the btr1-A and btr1-B domesticated alleles. Here, we extended the haplotype analysis to other T. turgidum subspecies and to the BTR1 homologues in the related T. timopheevii species. Our results showed that all the domesticated wheat subspecies within T. turgidum share common BTR1-A and BTR1-B haplotypes, confirming their common origin. In T. timopheevii, however, we identified a novel loss-of-function btr1-A allele underlying a partially brittle spike phenotype. This novel recessive allele appeared fixed within the pool of domesticated Timopheev’s wheat but was also carried by one wild timopheevii accession exhibiting partial brittleness. The promoter region for BTR1-B could not be amplified in any T. timopheevii accessions with any T. turgidum primer combination, exemplifying the gene-level distance between the two species. Altogether, our results support the concept of independent domestication processes for the two polyploid, wheat-related species.
Genetic Diversity and Population Structure Analysis of Triticum aestivum L. Landrace Panel from Afghanistan
Authors: Tehseen Muhammad Massub , Istipliler Deniz , Kehel Zakaria , Sansaloni Carolina P. , da Silva Lopes Marta , Kurtulus Ezgi , Muazzam Sana , Nazari Kumarse
Published: February 2021

Landraces are a potential source of genetic diversity and provide useful genetic resources to cope with the current and future challenges in crop breeding. Afghanistan is located close to the centre of origin of hexaploid wheat. Therefore, understanding the population structure and genetic diversity of Afghan wheat landraces is of enormous importance in breeding programmes for the development of high-yielding cultivars as well as broadening the genetic base of bread wheat. Here, a panel of 363 bread wheat landraces collected from seven north and north-eastern provinces of Afghanistan were evaluated for population structure and genetic diversity using single nucleotide polymorphic markers (SNPs). The genotyping-by-sequencing of studied landraces after quality control provided 4897 high-quality SNPs distributed across the genomes A (33.75%), B (38.73%), and D (27.50%). The population structure analysis was carried out by two methods using model-based STRUCTURE analysis and cluster-based discriminant analysis of principal components (DAPC). The analysis of molecular variance showed a higher proportion of variation within the sub-populations compared with the variation observed as a whole between sub-populations. STRUCTURE and DAPC analysis grouped the majority of the landraces from Badakhshan and Takhar together in one cluster and the landraces from Baghlan and Kunduz in a second cluster, which is in accordance with the micro-climatic conditions prevalent within the north-eastern agro-ecological zone. Genetic distance analysis was also studied to identify differences among the Afghan regions; the strongest correlation was observed for the Badakhshan and Takhar (0.003), whereas Samangan and Konarha (0.399) showed the highest genetic distance. The population structure and genetic diversity analysis highlighted the complex genetic variation present in the landraces which were highly correlated to the geographic origin and micro-climatic conditions within the agro-climatic zones of the landraces. The higher proportions of admixture could be attributed to historical unsupervised exchanges of seeds between the farmers of the central and north-eastern provinces of Afghanistan. The results of this study will provide useful information for genetic improvement in wheat and is essential for association mapping and genomic prediction studies to identify novel sources for resistance to abiotic and biotic stresses.
Lux et Tenebris? Coloniality and Anglican Missions in Argentine Patagonia in the Nineteenth Century
Authors: Córdova Quero Hugo
Published: February 2021

Within the modern capitalist World-System, Missionary work was mostly developed through the connubiality with colonial powers. The missionary work of the Anglican Church is no exception. This article centers on the missionary enterprise carried out in Argentine Patagonia in the nineteenth century. Missionaries’ reports carefully narrated that venture. However, the language and the notions underlying the missionary work’s narration reveal the dominion of colonial ideologies that imbued how religious agents constructed alterity. Connecting the missionaries’ worldview with the political context and expansion of the British Empire allows us to unfold the complex intersections of religious, ethnic, racial, and geopolitical discourses that traverse the lives of indigenous peoples in South America.
MALDI-TOF Protein Profiling Reflects Changes in Type 1 Diabetes Patients Depending on the Increased Amount of Adipose Tissue, Poor Control of Diabetes and the Presence of Chronic Complications
Authors: Zawada Agnieszka , Naskręt Dariusz , Matuszewska Eliza , Kokot Zenon , Grzymisławski Marian , Zozulińska-Ziółkiewicz Dorota , Dobrowolska Agnieszka , Matysiak Jan
Published: February 2021

Introduction: Protein profiling allows the determination of the presence of proteins marking various stages of the disease, and differentiates between people at risk of various diseases. In type 1 diabetes, protein profiling had been previously used to find blood markers other than islet autoantibodies to indicate the pancreatic beta cell destruction process and to reflect the progression of type 1 diabetes mellitus (T1DM). However, T1DM is an auto-immune disease and its clinical presentation changes in time of its duration. The aim of the study: To find differences in protein profiles in patients with type 1 diabetes according to diabetes control (HbA1c > 7%) and with presence of diabetic complications or obesity. It may help to identify subgroups of patients who may need a better clinical supervision and individualized treatment. Material and methods: A group of 103 patients with auto-immunologically confirmed T1DM, and meeting the following inclusion criteria: Caucasian race, duration of diabetes >5 years, were used in the study. Criteria of exclusion: past or present cancer (treated with chemo-/radiotherapy), diseases of the liver (ALT > 3 × ULN) except for people with simple hepatic steatosis, chronic renal disease (eGFR < 30 mL/1.73 m2/min), and acute inflammation (CRP > 5 mg/dL). The study group was divided in terms of the presence of chronic complications, obesity, or poor metabolic control (HbA1c > 7%). Protein profiling was completed by using the MALDI-TOF MS (matrix-assisted laser desorption/ionization-time of flight mass spectrometry) analyzer. Results: Differentiating proteins were identified in all of the groups. The groups burdened with complications, obesity, and poor metabolic control were characterized by increased levels of fibrinogen, complement C4 and C3. Conclusion: The groups of type 1 diabetes patients burdened with complications, obesity, and poor metabolic control were characterized by increased levels of fibrinogen, complement C4 and C3. Further detailed studies are necessary to determine more subtle changes in the proteomic profile of patients with type 1 diabetes.
Five-Year Enhanced Natural Attenuation of Historically Coal-Tar-Contaminated Soil: Analysis of Polycyclic Aromatic Hydrocarbon and Phenol Contents
Authors: Telesiński Arkadiusz , Kiepas-Kokot Anna
Published: February 2021

The objective of this study was to assess the soil pollution on an industrial wasteland, where coal-tar was processed in the period between 1880 and 1997, and subsequent to assess the decline in the content of phenols and polycyclic aromatic hydrocarbons (PAHs) during enhanced natural attenuation. The soil of the investigated area was formed from a layer of uncompacted fill. Twelve sampling points were established in the investigated area for collecting soil samples. A study conducted in 2015 did not reveal any increase in the content of heavy metals, monoaromatic hydrocarbons (BTEX), and cyanides. However, the content of PAHs and phenols was higher than the content permitted by Polish norms in force until 2016. In the case of PAHs, it was observed for individual compounds and their total contents. Among the various methods, enhanced natural attenuation was chosen for the remediation of investigated area. Repeated analyses of the contents of phenols and PAHs were conducted in 2020. The results of the analyses showed that enhanced natural attenuation has led to efficient degradation of the simplest substances—phenol and naphthalene. The content of these compounds in 2020 was not elevated compared to the standards for industrial wastelands. The three- and four-ring hydrocarbons were degraded at a lower intensity. Based on the mean decrease in content after 5-year enhanced natural attenuation, the compounds can be arranged in the following order: phenols > naphthalene > phenanthrene > fluoranthene > benzo(a)anthracene > chrysene > anthracene.
Chilean Digital Press Coverage of the Relation between Diet and Mental Health
Authors: Sanchez-Sabate Ruben , Zunino Esteban , Badilla-Briones Yasna , Celedon Celis Natalia , Caro Saldías Daniel
Published: February 2021

Chile has a serious public health problem due to the high prevalence of both unhealthy dietary patterns and mental illnesses. Given that dietary quality is positively associated with the quality of mental health, it is urgent that healthy dietary patterns be promoted among Chileans. The WHO recommends the use of mass media for the dissemination of knowledge about mental health. Since health news affect people’s attitudes and health behaviors, this study analyzed the coverage by three Chilean online newspapers with the largest readership regarding the relation between diet and mental health in 2016. A previously constructed corpus of 2551 news items about food was analyzed quantitatively. The results show that the relevance of the topic diet and mental health was low in all three newspapers. The most frequent type of information was on “foods” and not “nutrients” that “benefit”—not that “damage”—mental health. The quality of the news was poor as a narrow range of sources was found. An individual responsibility frame predominated in the information to the detriment of a public health frame.
A Walk-In Clinic for Newly Arrived Mentally Burdened Refugees: The Patient Perspective
Authors: Zehetmair Catharina , Zeyher Valentina , Cranz Anna , Ditzen Beate , Herpertz Sabine C. , Kohl Rupert Maria , Nikendei Christoph
Published: February 2021

Providing refugees with psychosocial support is particularly important considering the high level of mental health problems prevalent in this population. A psychosocial walk-in clinic operating within a state reception and registration center in Germany has been supporting mentally burdened refugees since 2016. This study focused on patients’ perspectives on their mental health burden, the psychosocial walk-in clinic, and future help seeking. We conducted interviews with n = 22 refugees attending the walk-in clinic from March to May 2019. Qualitative analysis focused on the following four topics: (1) mental burden from the patients’ perspective, (2) access to the psychosocial walk-in clinic, (3) perception of counseling sessions, and (4) perception of follow-up treatment. The results show that the majority of interviewees were burdened by psychological and somatic complaints, mostly attributed to past experiences and post-migratory stress. Therapeutic counseling and psychiatric medication were found to be particularly helpful. Most of the participants felt motivated to seek further psychosocial support. Key barriers to seeking psychosocial help included shame, fear of stigma, and lack of information. Overall, the psychosocial walk-in clinic is a highly valued support service for newly arrived refugees with mental health issues.
A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture
Authors: Sivanesan Iyyakkannu , Muthu Manikandan , Gopal Judy , Tasneem Shadma , Kim Doo-Hwan , Oh Jae-Wook
Published: February 2021

Plant tissue culture has led to breakthroughs in understanding and applying the fundamental knowledge towards harnessing more from plants. Microbial contamination is one of the serious problems limiting the successful extrapolation of plant tissue culture practices. Sources of in vitro contamination include culture containers, media, explants, equipment, the environment of the culture room and transfer area, and operating personnel. The successful initiation of in vitro culture mostly depends on surface sterilization of explants because this is the primary source. Usually, surface sterilization is done using chemicals, or toxic nanomaterials, this is the first time such an approach has been demonstrated. Numerous surface microflora attached to plant surfaces grow faster than the cultured explants and release phytotoxic substances into the culture media, hindering positive outcomes. In the current work, for the first time, the applicability of turmeric and benzoin resin-based fumigation of explants is demonstrated. The results showed that fumigation methods for surface sterilization were promising and could lead to fifty and even 100% contamination-free plant tissue culture. Nanoparticulate carbon was identified in the turmeric and benzoin smoke and coined the key player in the surface sterilization effect. These studies open a whole new avenue for the use of fumigation-based methods for riddance of microbial contamination.
Erratum: Alves, J., et al. Correlations between Basal Trace Minerals and Hormones in Middle and Long-Distance High-Level Male Runners. International Journal of Environmental Research and Public Health 2020, 17, 9473
Authors: Alves Javier , Barrientos Gema , Toro Víctor , Grijota Francisco Javier , Muñoz Diego , Maynar Marcos
Published: February 2021

The authors wish to correct the following erratum in this paper [...]
Cultivation of the Acidophilic Microalgae Galdieria phlegrea with Wastewater: Process Yields
Authors: di Cicco Maria Rosa , Palmieri Maria , Altieri Simona , Ciniglia Claudia , Lubritto Carmine
Published: February 2021

Algal based wastewater treatment offers the opportunity to recover, in the form of biomass, the nutrients and internal chemical energy of wastewater. Recently, there has been a growing interest in the use of extremophilic microalgae, as they can easily adapt to difficult and often pollutant-rich environments. The thermo-acidophilic microalga Galdieria phlegrea is a species of recent discovery and great metabolic versatility, but it has still been poorly studied. Here, G. phlegrea was cultivated using raw municipal wastewater in 1 L Erlenmeyer flasks with 700 mL working volume at 37 °C for up to nine days. During the cultivation phase, biomass growth, phycocyanin content, ammonium and phosphate removal from the wastewater, lipid fraction, total carbon and nitrogen in the biomass, and variation in δ13C and δ15N isotopic ratios (a novel analytical contribution in these experiments) were monitored. Results indicated that G. phlegrea was able to grow in raw effluent, where it removed more than 50% ammonium and 20% phosphate in 24 h; total lipid content was in the range of 11–22%, while average C-N content was of 45% and 6%, respectively; isotopic analyses proved to be a useful support in identifying C and N metabolic pathways from effluent to biomass. Overall, G. phlegrea showed consistent performance with similar Cyanidiophyceae and is a potentially viable candidate for municipal wastewater valorization from a circular economy perspective.
“They Are Worth Their Weight in Gold”: Families and Clinicians’ Perspectives on the Role of First Nations Health Workers in Paediatric Burn Care in Australia
Authors: Coombes Julieann , Fraser Sarah , Hunter Kate , Ivers Rebecca , Holland Andrew , Grant Julian , Mackean Tamara
Published: February 2021

Burns affect Australia’s First Nations children more than other Australian children, they also experience longer lengths of stay in tertiary burns units and face barriers in accessing burn aftercare treatment. Data sets from two studies were combined whereby 19 families, 11 First Nations Health Worker (FNHW) and 56 multidisciplinary burn team members from across Australia described the actual or perceived role of FNHW in multidisciplinary burn care. Data highlighted similarities between the actual role of FNHW as described by families and as described by FNHW such as enabling cultural safety and advocacy. In contrast, a disconnect between the actual experience of First Nations families and health workers and that as perceived by multidisciplinary burn team members was evident. More work is needed to understand the impact of this disconnect and how to address it.
Restoration of Visitors through Nature-Based Tourism: A Systematic Review, Conceptual Framework, and Future Research Directions
Authors: Qiu Mengyuan , Sha Ji , Scott Noel
Published: February 2021

Visiting natural environments could restore health and contribute to human sustainability. However, the understanding of potential linkages between restoration of visitors and nature-based tourism remains incomplete, resulting in a lack of orientation for researchers and managers. This study aimed to explore how visitors achieve restoration through nature by analyzing published literature on tourism. Using a systematic review method, this study examined destination types, participant traits, theoretical foundations, and potential restorative outcomes presented in 34 identified articles. A new framework that synthesizes relevant research and conceptualizes the restorative mechanisms of nature-based tourism from a human–nature interaction perspective was developed. Owing to the limitations in the theories, methods, cases, and the COVID-19 pandemic, interdisciplinary methods and multisensory theories are needed in the future to shed further light on the restoration of visitors through nature-based tourism. The findings provide a theoretical perspective on the consideration of nature-based tourism as a public-wellness product worldwide, and the study provides recommendations for future research in a COVID-19 or post-COVID-19 society.
Exposure to PM2.5 and Obesity Prevalence in the Greater Mexico City Area
Authors: Tamayo-Ortiz Marcela , Téllez-Rojo Martha María , Rothenberg Stephen J. , Gutiérrez-Avila Ivan , Just Allan Carpenter , Kloog Itai , Texcalac-Sangrador José Luis , Romero-Martinez Martin , Bautista-Arredondo Luis F. , Schwartz Joel , Wright Robert O. , Riojas-Rodriguez Horacio
Published: February 2021

Exposure to PM2.5 has been associated with the prevalence of obesity. In the Greater Mexico City Area (GMCA), both are ranked among the highest in the world. Our aim was to analyze this association in children, adolescents, and adults in the GMCA. We used data from the 2006 and 2012 Mexican National Surveys of Health and Nutrition (ENSANUT). Participants’ past-year exposure to ambient PM2.5 was assessed using land use terms and satellite-derived aerosol optical depth estimates; weight and height were measured. We used survey-adjusted logistic regression models to estimate the odds ratios (ORs) of obesity (vs. normal-overweight) for every 10 µg/m3 increase in annual PM2.5 exposure for children, adolescents, and adults. Using a meta-analysis approach, we estimated the overall odds of obesity. We analyzed data representing 19.3 million and 20.9 million GMCA individuals from ENSANUT 2006 and 2012, respectively. The overall pooled estimate between PM2.5 exposure and obesity was OR = 1.96 (95% CI: 1.21, 3.18). For adolescents, a 10 µg/m3 increase in PM2.5 was associated with an OR of 3.53 (95% CI: 1.45, 8.58) and 3.79 (95% CI: 1.40, 10.24) in 2006 and 2012, respectively. More studies such as this are recommended in Latin American cities with similar air pollution and obesity conditions.
Curve Sprint in Elite Female Soccer Players: Relationship with Linear Sprint and Jump Performance
Authors: Kobal Ronaldo , Freitas Tomás T. , Fílter Alberto , Requena Bernardo , Barroso Renato , Rossetti Marcelo , Jorge Renato M. , Carvalho Leonardo , Pereira Lucas A. , Loturco Irineu
Published: February 2021

The aim of this study was to examine the associations between linear sprint, curve sprint (CS), change of direction (COD) speed, and jump performance in a sample of 17 professional female soccer players. All athletes performed squat and countermovement jumps, single leg horizontal triple jumps, 17 m linear sprints, CS tests, and a 17 m Zigzag COD test. A Pearson product–moment test was performed to determine the relationships among the assessed variables. The significance level was set at p < 0.05. Nearly perfect associations (r > 0.9) were found between linear and CS velocities. Players faster in linear sprints and CS exhibited greater COD deficits. No significant associations were found between COD deficit and either body mass or sprint momentum. Jumping ability was significantly correlated with linear sprint and CS performance, but not to COD performance. These findings may be used by coaches and practitioners to guide testing and training prescriptions in this population. The associations observed here suggest that training methods designed to improve linear sprint and CS velocities may benefit from the implementation of vertically and horizontally oriented plyometric exercises.
Implementation of Bio-Risk Management System in a National Clinical and Medical Referral Centre Laboratories
Authors: Lestari Fatma , Kadir Abdul , Miswary Thariq , Maharani Cynthia Febrina , Bowolaksono Anom , Paramitasari Debby
Published: February 2021

The increasing threats from biological agents have become a concern in laboratories, and emerging infectious diseases have demanded increased awareness and preparedness of laboratory facilities. Bio-risk assessment is needed to provide a framework for organisations to establish a comprehensive bio-risk management system. The assessment criteria should include both biosafety and biosecurity measures. Laboratories in Indonesia play a significant role in public health interventions in term of disease screening, diagnosis and medical decision making. The National Clinical and Medical Referral Centre Laboratories have the potential of daily exposures to dangerous biological materials. This study aims to identify the gap between bio-risk management system implementation and International Standard Organisation (ISO) 35001:2019 requirements. The 202 items in ISO 35001:2019 are categorized into seven main elements. The findings show that more than half of the elements on ISO 35001:2019 have been implemented in these centres. Good performance was identified at lab 4 and 5 which obtained the highest scores, particularly in the context of organisation, planning, operation and improvement elements. However, the widest gap was found in leadership, support and performance evaluation. One way to address this would be to create written rules and regulations at the laboratory top management level to require all laboratory facilities to comply to the bio-risk policies, rules, and regulations.
Prolonged Maternal and Child Health, Food and Nutrition Problems after the Kumamoto Earthquake: Semantic Network Analysis of Interviews with Dietitians
Authors: Tsuboyama-Kasaoka Nobuyo , Hamada Mari , Ohnishi Kae , Ueda Sakiko , Ito Yukako , Nakatani Hisae , Sudo Noriko , Noguchi Ritsuna
Published: February 2021

Infants need sufficient nutrients even during disasters. Only qualitative descriptive analysis has been reported regarding nutritional problems of mothers and children after the Kumamoto earthquake, and non-subjective analysis is required. This study examined issues concerning maternal and child health, food and nutrition after the Kumamoto earthquake using automatic computer quantitative analysis from focus group interviews (FGIs). Study participants (n = 13) consisted of dietitians in charge of nutrition assistance of infants in affected areas. The content of the interviews was converted into text, nouns were extracted, and co-occurrence network diagram analysis was performed. In the severely damaged area, there were hygienic problems not only in the acute phase but also in the mid-to-long-term phase. “Allergy” was extracted in the surrounding area in the acute and the mid-to-long-term phase, but not in the severely damaged area as the acute phase issue. In the surrounding area, problems have shifted to health and the quality of diet in the mid-to-long-term phase. This objective analysis suggested that dietary problems for mothers and children after disaster occurred also in the mid-to-long-term phase. It will be necessary to combine the overall trends obtained in this study with the results of qualitative descriptive analysis.
Chronic Exposure to Organophosphates Pesticides and Risk of Metabolic Disorder in Cohort from Pakistan and Cameroon
Authors: Leonel Javeres Mbah Ntepe , Habib Rabia , Judith Laure Ngondi , Abbas Shah Syed Tahir , Valis Martin , Kuca Kamil , Muhammad Nurulain Syed
Published: February 2021

(1) Background: Organophosphorus pesticides (OPPs) are major chemicals used in agriculture for eradication of insecticides/pesticides. Unfortunately, the longtime exposure of human beings to OPPs could lead to metabolic disorder such as high blood pressure, hyperglycemia, overweight or dyslipidemia. The aim of this research is to evaluate the possible metabolic dysregulations as a consequence of chronic OPPs exposure to individuals in Cameroon and Pakistan. (2) Methods: Blood samples were collected from 300 participants in each country, into ethylenediaminetetraacetic acid (EDTA) tubes. The samples were extracted with solid phase extraction (methanol/water) for analysis of OPPs with gas chromatography mass spectrometry. The spectrophotometry and Enzyme Linked ImmunoSorbent Assay (ELISA) were used to measure the hepatic, renal, pancreatic and cardiovascular functions. The atherogenic index (AI) was also determined in OPPs exposed and nonexposed cohorts. (3) Results: The results showed the presence of malathion, parathion and chlorpyrifos OPPs residues in Cameroonians, and malathion and chlorpyrifos in Pakistani samples, respectively. Elevated Body Mass Index (BMI), insulin, blood glucose, dyslipidemia and hypertension were noted in OPPs chronic exposed groups. In addition, dysregulated liver and kidney function profiles were observed in all participants regardless of gender and age groups. (4) Conclusions: The study concludes that both the study cohorts showed several metabolic dysregulations attributable to chronic exposure to a mixture of OPPs which may provide precursors for establishment of metabolic syndrome and other chronic diseases. Further different extended population-based studies are suggested to understand the differential metabolic dysfunctions caused by structurally different OPPs mixtures exposure.
Responsiveness and Minimal Clinically Important Difference of the Five Times Sit-to-Stand Test in Patients with Stroke
Authors: Agustín Rodrigo Martín-San , Crisostomo Mª José , Sánchez-Martínez Mª Piedad , Medina-Mirapeix Francesc
Published: February 2021

This study aimed to analyze the responsiveness of the 5STS test among stroke patients and to estimate the MCIDs (minimal clinically important differences) for different severity levels of community ambulation and stages of recovery. The 5STS and comparator instruments (gait speed and Functional Ambulatory Category (FAC)) were evaluated at baseline. These measures were repeated at 4 (Stage 1) and 8 weeks (Stage 2), together with the Global Rating of Change (GROC). The MCIDs were calculated with two anchor-based methods using the GROC as the external criterion. Responsiveness to change for the 5STS was estimated analyzing the correlation with changes in the two comparator instruments and their capacity to discriminate improvement. For the 5STS test, while the MCIDs of the limited community ambulators were similar in the two stages (around 3 s), those of the household ambulators decreased from 1.9 s to 0.72 s. Spearman’s rho coefficients showed an acceptable correlation between changes in 5STS and changes for both the FAC and gait speed changes in both stages of recovery. Our study revealed that the 5STS is responsive to functional changes in patients with stroke and that their degree of severity and stage of recovery influence the MCID values of the 5STS.
Development of a Structured Interview to Explore Interpersonal Schema of Older Adults Living Alone Based on Autobiographical Memory
Authors: Kwan Yunna , Choi Sungwon , Eom Tae Rim , Kim Tae Hui
Published: February 2021

With a growing public interest in the social health of older adults, studies focusing on social networks and interpersonal relationships of older adults are needed. The present study was conducted to develop a structured interview to evaluate the interpersonal schema based on Self-Defining Memory of older adults. First, the word cues that the older adults often report on interpersonal events were confirmed. Next, the indices and scoring rules were prepared, including Relationship frequency (RF), Conflict frequency (CF), Dominance mean (Dm), and Warmth mean (Wm). Healthy older adults living alone (mean age = 71.81, SD = 3.95) were interviewed. Finally, the correlation between each index and Short form of Korean Inventory of Interpersonal Problems Circumplex Scales (KIIP-SC) was analyzed for criterion validity. The inter-rater reliability was substantial (Kappa = 0.61~0.66). Based on the analysis of criterion validity, the indices of CF, Dm, and Wm indices showed an appropriate level of criterion validity. This study developed a structural interview based on a novel system of reporting autobiographical memory and established indices with appropriate validity to evaluate interpersonal relationships. The interview is expected to identify the characteristics of interpersonal relationships of the older adults and contribute to the establishment of the older adults’ community accordingly.
Dental Pulp-Derived Mesenchymal Stem Cells for Modeling Genetic Disorders
Authors: Masuda Keiji , Han Xu , Kato Hiroki , Sato Hiroshi , Zhang Yu , Sun Xiao , Hirofuji Yuta , Yamaza Haruyoshi , Yamada Aya , Fukumoto Satoshi
Published: February 2021

A subpopulation of mesenchymal stem cells, developmentally derived from multipotent neural crest cells that form multiple facial tissues, resides within the dental pulp of human teeth. These stem cells show high proliferative capacity in vitro and are multipotent, including adipogenic, myogenic, osteogenic, chondrogenic, and neurogenic potential. Teeth containing viable cells are harvested via minimally invasive procedures, based on various clinical diagnoses, but then usually discarded as medical waste, indicating the relatively low ethical considerations to reuse these cells for medical applications. Previous studies have demonstrated that stem cells derived from healthy subjects are an excellent source for cell-based medicine, tissue regeneration, and bioengineering. Furthermore, stem cells donated by patients affected by genetic disorders can serve as in vitro models of disease-specific genetic variants, indicating additional applications of these stem cells with high plasticity. This review discusses the benefits, limitations, and perspectives of patient-derived dental pulp stem cells as alternatives that may complement other excellent, yet incomplete stem cell models, such as induced pluripotent stem cells, together with our recent data.
Leaf-to-Whole Plant Spread Bioassay for Pepper and Ralstonia solanacearum Interaction Determines Inheritance of Resistance to Bacterial Wilt for Further Breeding
Authors: Kwon Ji-Su , Nam Jae-Young , Yeom Seon-In , Kang Won-Hee
Published: February 2021

Bacterial wilt (BW) disease from Ralstonia solanacearum is a serious disease and causes severe yield losses in chili peppers worldwide. Resistant cultivar breeding is the most effective in controlling BW. Thus, a simple and reliable evaluation method is required to assess disease severity and to investigate the inheritance of resistance for further breeding programs. Here, we developed a reliable leaf-to-whole plant spread bioassay for evaluating BW disease and then, using this, determined the inheritance of resistance to R. solanacearum in peppers. Capsicum annuum ‘MC4′ displayed a completely resistant response with fewer disease symptoms, a low level of bacterial cell growth, and significant up-regulations of defense genes in infected leaves compared to those in susceptible ‘Subicho’. We also observed the spreading of wilt symptoms from the leaves to the whole susceptible plant, which denotes the normal BW wilt symptoms, similar to the drenching method. Through this, we optimized the evaluation method of the resistance to BW. Additionally, we performed genetic analysis for resistance inheritance. The parents, F1 and 90 F2 progenies, were evaluated, and the two major complementary genes involved in the BW resistance trait were confirmed. These could provide an accurate evaluation to improve resistant pepper breeding efficiency against BW.
Effect of Varying Amount of Polyethylene Glycol (PEG-600) and 3-Aminopropyltriethoxysilane on the Properties of Chitosan based Reverse Osmosis Membranes
Authors: Kayani Anum , Raza Muhammad Asim , Raza Arsalan , Hussain Tajamal , Akram Muhammad Sarfraz , Sabir Aneela , Islam Atif , Haider Bilal , Khan Rafi Ullah , Park Sang Hyun
Published: February 2021

Chitosan and polyethylene glycol (PEG-600) membranes were synthesized and crosslinked with 3-aminopropyltriethoxysilane (APTES). The main purpose of this research work is to synthesize RO membranes which can be used to provide desalinated water for drinking, industrial and agricultural purposes. Hydrogen bonding between chitosan and PEG was confirmed by displacement of the hydroxyl absorption peak at 3237 cm−1 in pure chitosan to lower values in crosslinked membranes by using FTIR. Dynamic mechanical analysis revealed that PEG lowers Tg of the modified membranes vs. pure chitosan from 128.5 °C in control to 120 °C in CS-PEG5. SEM results highlighted porous and anisotropic structure of crosslinked membranes. As the amount of PEG was increased, hydrophilicity of membranes was increased and water absorption increased up to a maximum of 67.34%. Permeation data showed that flux and salt rejection value of the modified membranes was increased up to a maximum of 80% and 40.4%, respectively. Modified films have antibacterial properties against Escherichia coli as compared to control membranes.
Targeting Epigenetic Regulators for Endometrial Cancer Therapy: Its Molecular Biology and Potential Clinical Applications
Authors: Inoue Futaba , Sone Kenbun , Toyohara Yusuke , Takahashi Yu , Kukita Asako , Hara Aki , Taguchi Ayumi , Tanikawa Michihiro , Tsuruga Tetsushi , Osuga Yutaka
Published: February 2021

Endometrial cancer is one of the most frequently diagnosed gynecological malignancies worldwide. However, its prognosis in advanced stages is poor, and there are only few available treatment options when it recurs. Epigenetic changes in gene function, such as DNA methylation, histone modification, and non-coding RNA, have been studied for the last two decades. Epigenetic dysregulation is often reported in the development and progression of various cancers. Recently, epigenetic changes in endometrial cancer have also been discussed. In this review, we give the main points of the role of DNA methylation and histone modification in endometrial cancer, the diagnostic tools to determine these modifications, and inhibitors targeting epigenetic regulators that are currently in preclinical studies and clinical trials.
Activation of the Interleukin-33/ST2 Pathway Exerts Deleterious Effects in Myxomatous Mitral Valve Disease
Authors: Garcia-Pena Amaia , Ibarrola Jaime , Navarro Adela , Sadaba Alba , Tiraplegui Carolina , Garaikoetxea Mattie , Arrieta Vanessa , Matilla Lara , Fernández-Celis Amaya , Sadaba Rafael , Alvarez Virginia , Gainza Alicia , Jover Eva , López-Andrés Natalia
Published: February 2021

Mitral valve disease (MVD) is a frequent cause of heart failure and death worldwide, but its etiopathogenesis is not fully understood. Interleukin (IL)-33 regulates inflammation and thrombosis in the vascular endothelium and may play a role in the atherosclerotic process, but its role in mitral valve has not been investigated. We aim to explore IL-33 as a possible inductor of myxomatous degeneration in human mitral valves. We enrolled 103 patients suffering from severe mitral regurgitation due to myxomatous degeneration undergoing mitral valve replacement. Immunohistochemistry of the resected leaflets showed IL-33 and ST2 expression in both valve interstitial cells (VICs) and valve endothelial cells (VECs). Positive correlations were found between the levels of IL-33 and molecules implicated in the development of myxomatous MVD, such as proteoglycans, extracellular matrix remodeling enzymes (matrix metalloproteinases and their tissue inhibitors), inflammatory and fibrotic markers. Stimulation of single cell cultures of VICs and VECs with recombinant human IL-33 induced the expression of activated VIC markers, endothelial–mesenchymal transition of VECs, proteoglycan synthesis, inflammatory molecules and extracellular matrix turnover. Our findings suggest that the IL-33/ST2 system may be involved in the development of myxomatous MVD by enhancing extracellular matrix remodeling.
Strategies to Overcome the Barrier of Ischemic Microenvironment in Cell Therapy of Cardiovascular Disease
Authors: Berndt Rouven , Albrecht Martin , Rusch René
Published: February 2021

The transplantation of various immune cell types are promising approaches for the treatment of ischemic cardiovascular disease including myocardial infarction (MI) and peripheral arterial disease (PAD). Major limitation of these so-called Advanced Therapy Medicinal Products (ATMPs) is the ischemic microenvironment affecting cell homeostasis and limiting the demanded effect of the transplanted cell products. Accordingly, different clinical and experimental strategies have been evolved to overcome these obstacles. Here, we give a short review of the different experimental and clinical strategies to solve these issues due to ischemic cardiovascular disease.
Baicalin-Induced Autophagy Preserved LPS-Stimulated Intestinal Cells from Inflammation and Alterations of Paracellular Permeability
Authors: Rizzo Valentina , Ferlazzo Nadia , Currò Monica , Isola Gaetano , Matarese Marco , Bertuccio Maria Paola , Caccamo Daniela , Matarese Giovanni , Ientile Riccardo
Published: February 2021

Several studies have demonstrated a relevant role of intestinal epithelial cells in the immune response and in chronic inflammatory conditions, including ulcers, colitis, and Crohn’s disease. Baicalin (BA), extracted from the root of Scutellaria baicalensis, has various beneficial healthy effects, including anti-inflammatory activity. However, few studies have evaluated BA effects on autophagic signaling in epithelial cell response to inflammatory stimuli. To explore possible beneficial effects of BA, HT-29 cells were exposed to lipopolysaccharide (LPS), in presence or absence of BA, for 4 h. We evaluated mRNA levels of autophagy-related genes and cytokines, triggering inflammatory response. Furthermore, the expression of claudin 1, involved in the regulation of paracellular permeability was analyzed. BA treatment repressed LPS-induced expression of TNF-α and IL-1β. The down-regulation of autophagy-related genes induced by LPS was counteracted by cell pretreatment with BA. Under these conditions, BA reduced the NF-κB activation caused by LPS. Also, BA restored mRNA and protein levels of claudin 1, which were reduced by LPS. In conclusion, in intestinal epithelial cells BA regulates the NF-κB activation and modulates both autophagic and inflammatory processes, leading to an improvement of paracellular permeability. These results suggest that the anti-inflammatory effects of BA can be associated to the regulation of autophagic flux.