bluuebunny/crossref_metadata_2025 huggingface dataset:
Pseudo-Steady-State Parameters for a Well Penetrated by a Fracture with an Azimuth Angle in an Anisotropic Reservoir
Authors: Xing Guoqiang , Wang Mingxian , Wu Shuhong , Li Hua , Dong Jiangyan , Zhao Wenqi
Published: June 2019

Many oil wells in closed reservoirs continue to produce in the pseudo-steady-state flow regime for a long time. The principal objective of this work is to investigate the characteristics of two key pseudo-steady-state parameters—pseudo-steady-state constant (bDpss) and pseudo-skin factor (S)—for a well penetrated by a fracture with an azimuth angle (θ) in an anisotropic reservoir. Firstly, a general analytical pressure solution for a finite-conductivity fracture with or without an azimuth angle in an anisotropic rectangular reservoir was developed by using the point-source function and spatial integral method, and two typical cases were employed to verify this solution. Secondly, with the asymptotic analysis method, the expressions of pseudo-steady-state constant and pseudo-skin factor were obtained on the basis of their definitions, and the effects of permeability anisotropy, fracture azimuth angle, fracture conductivity and reservoir shape on them were discussed in detail. Results show that all the bDpss-θ and S-θ curves are symmetric around the vertical line, θ = 90° and form a hump or groove shape. The optimized fracture direction in an anisotropic reservoir is perpendicular to the principal permeability axis. Furthermore, a new formula to calculate pseudo-skin factor was successfully proposed based on these two parameters’ relationship. Finally, as an application of pseudo-steady-state constant, a set of Blasingame format rate decline curves for the proposed model were established.
Tip-Bed Velocity and Scour Depth of Horizontal-Axis Tidal Turbine with Consideration of Tip Clearance
Authors: Zhang Tianming , Lam Wei Haur , Cui Yonggang , Jiang Jinxin , Sun Chong , Guo Jianhua , Ma Yanbo , Wang Shuguang , Lam Su Shiung , Hamill Gerard
Published: June 2019

The scouring by a tidal turbine is investigated by using a joint theoretical and experimental approach in this work. The existence of a turbine obstructs a tidal flow to divert the flow passing through the narrow channel in between the blades and seabed. Flow suppression is the main cause behind inducing tidal turbine scouring, and its accelerated velocity is being termed as tip-bed velocity (Vtb). A theoretical equation is currently proposed to predict the tip-bed velocity based on the axial momentum theory and the conservation of mass. The proposed tip-bed velocity equation is a function of four variables of rotor radius (r), tip-bed clearance (C), efflux velocity (V0) and free flow velocity (V∞), and a constant of mass flow coefficient (Cm) of 0.25. An experimental apparatus was built to conduct the scour experiments. The results provide a better understanding of the scour mechanism of the horizontal axis tidal turbine-induced scour. The experimental results show that the scour depth is inversely proportional to tip-bed clearance. Turbine coefficient (Kt) is proposed based on the relationship between the tip-bed velocity and the experimental tidal turbine scour depth. Inclusion of turbine coefficient (Kt) into the existing pier scour equations can predict the maximum scour depth of a tidal turbine with an error range of 5–24%.
Reasons for the Extremely Small Population of putative hybrid Sonneratia × hainanensis W.C. Ko (Lythraceae)
Authors: Zhang Mengwen , Yang Xiaobo , Long Wenxing , Li Donghai , Lv Xiaobo
Published: June 2019

Sonneratia × hainanensis, a species once endemic to Hainan Island in China, is now endangered. China’s State Forestry Administration lists this species as a wild plant species with an extremely small population. Field fixed-point investigations, artificial pollination, and laboratory experiments, as well as other methods, were applied to study the reproductive system and seed germination of S. × hainanensis to elucidate the reasons for the endangerment of this species. The results are as follows: (1) Outcrossing index, pollen-ovule ratio, and artificial pollination showed S. × hainanensis has a mixed mating system and mainly focuses on outcrossing with some self-compatibility. (2) Fruit and seed placement tests showed that the fruit predators on the ground were mainly Fiddler crab and squirrel, with the predation rates being 100%. The artificially spread seeds do not germinate under natural conditions. The mean seed destruction rate and remaining rate of were 82.5% and 17.5%. (3) Seeds need to germinate under ambient light conditions, with an optimal photoperiod of 12 h. Seed germination is extremely sensitive to low temperatures because of optimum temperatures from 30 °C to 40 °C. At an optimal temperature of 35 °C, the seeds germinate under salinities ranging from 0‰ to 7.5‰, with an optimal salinity of 2.5‰, which shows the sensitivity of seed germination to salinity, with low salinity promoting germination, whereas high salinity inhibits germination. These findings indicate that the limited regeneration of S. × hainanensis is caused by the following: (1) Pollen limitation and inbreeding recession caused by the extremely small population of S. × hainanensis. (2) Seeds near parent trees are susceptible not only to high fruit drop rate, but to high predation beneath the parent trees′ canopy as well. (3) Seed germination has weak adaptability to light, temperature, and salinity.
Relationship between Parental Socialization, Emotional Symptoms, and Academic Performance during Adolescence: The Influence of Parents’ and Teenagers’ Gender
Authors: Bully Paola , Jaureguizar Joana , Bernaras Elena , Redondo Iratxe
Published: June 2019

Scientific interest in students’ emotional and psychosocial experiences has been increasing in the last years due to their influence on students’ learning processes and academic performance. The present manuscript tries to go further in the study of the relationship between perceived parenting socialization and academic performance by analyzing not only their direct effects, but also by testing their indirect influence through other variables such as students’ psychological and school maladjustment, especially focusing on gender differences (both of students and parents). The sample comprised 823 students (416 males and 407 females) from the Basque Country (Spain), with ages ranging between 12 and 16 years (M = 13.7, SD = 1.2). Students completed a sociodemographic data form, the PARQ-Control questionnaire, and the BASC-S3 test. Teachers answered an ad hoc question on each student’s academic performance. The data showed that, both for males and females, the same structure of parent–teenager relationship predicted teenagers’ academic performance, via psychological and school maladjustment. However, the intensity of the relationship between parental acceptance and teenagers’ results in all the other factors differed depending on teenagers’ gender. Fathers’ influence was greater in males, and mothers’ influence was higher in females. This study is considered a starting point for a theoretical model predicting academic performance and psychological and school maladjustment among teenagers.
The Effect of Environmental Performance on Employment: Evidence from China’s Manufacturing Industries
Authors: Shan Wei , Wang Jingyi
Published: June 2019

This research aims to explore the interaction between environmental performance and employment China’s manufacturing industries. Based on the environmental performance of 32 industries in China’s manufacturing industry during 2006–2015, a panel vector autoregressive model was constructed to study the interaction between industry output and employment in clean industries and dirty industries. The dynamic impact and internal transmission mechanism between environmental performance is analyzed. The study found that in the early stage, due to the reduction of production scale, there was a weak and short-term negative correlation effect on employment, and the mutual promotion relationship between economic benefits and employment was unsustainable. In return, employment affects environmental performance, but the effect differs due to the different forms of environmental performance. For dirty industries, the impact of environmental performance on employment through technical effects is more significant and, thus, a win–win situation of ecological environment and employment stability will be achieved. This research has practical significance regarding how to scientifically and effectively carry out environmental regulation and green management.
What Frequency of Technical Activity Is Needed to Improve Results? New Approach to Analysis of Match Status in Professional Soccer
Authors: Konefał Marek , Chmura Paweł , Rybka Kacper , Chmura Jan , Huzarski Maciej , Andrzejewski Marcin
Published: June 2019

The aim of the research detailed here has been to assess the frequency with which football players engage in technical activity of various different types, in relation to seven phases of a game associated with changes in match status. To this end, 2016–2017 domestic-season matches in Germany’s Bundesliga were analyzed, the relevant data being retrieved using an Opta Sportsdata Company system. Technical activity taken into consideration included shots, passes, ball possession, dribbles, and tackles. It was found that there was a large impact of frequency of shots on target (H = 466.999(6); p = 0.001) in relation to the different match-status phases. Furthermore, moderate effect sizes were then obtained for frequency of shots (H = 187.073(6); p = 0.001), frequency of passes (H = 133.547(6); p = 0.001), and percentage of ball possession (H = 123.401(6); p = 0.001). The implication would be that a team trying to change the match score of a game experienced at a given moment in a more favorable direction will need to raise the frequency and accuracy of passes, the percentage of ball possession, and the percentage of tackles ending in success. The maintenance of a winning match status requires a high frequency of occurrence of shots and shots on target as well as greater frequency and effectiveness of dribbling. The main finding from our work is that consideration of the consequences of a game presented in relation to seven potential phases to match status can point to a novel approach to analysis.
Normative Data on Grip Strength in a Population-Based Study with Adjusting Confounding Factors: Sixth Korea National Health and Nutrition Examination Survey (2014–2015)
Authors: Lim Seong Hoon , Kim Yeo Hyung , Lee Jung Soo
Published: June 2019

Background: We investigated population-based data on grip strength, analyzed for demographic factors, and proposed a formula to estimate grip strength that could be generalized to a population with different anthropometric and background characteristics. Methods: This study used a complex, stratified, multistage probability cluster survey with a representative sample of the population. Select household Korean participants (n = 6577) over age 10 who were able to perform daily tasks without issue were included. Grip strength was measured in both hands, alternately, three times using a digital grip strength dynamometer. Results: There was a curvilinear relationship between grip strength and age, and grip strength was higher in males than females (p = 0.001). Hand preference significantly affected grip strength (p = 0.001). Weight and height were positively correlated with strength in both hands (p = 0.001), but waist circumference was negatively correlated with strength in both hands (p = 0.001). The intensity of occupational labor did significantly affect grip strength in both hands (p = 0.001). The formulas for estimating grip strength of each hand are presented as main results. Conclusions: To determine normative data on grip strength, we may consider factors such as occupations with different physical demands, underlying medical conditions, anthropometric characteristics, and unmodifiable factors such as age and sex.
Evaluation of the Effectiveness of a Nursing/Physiotherapy Program in Chronic Patients
Authors: López-Liria Remedios , Vega-Ramírez Francisco Antonio , Aguilar-Parra José Manuel , Padilla-Góngora David , Trigueros-Ramos Rubén , Rocamora-Pérez Patricia
Published: June 2019

This study aimed to evaluate the functional impact of a shared intervention model by the mobile physiotherapy and rehabilitation team (MPRT) and primary care case management nurses (PCCMNs) on chronic patients. This was a prospective, observational study involving 1086 patients (mean age, 80 years; 63.7% females) in the province of Almeria, which was conducted between 2004 and 2018. Most of the registered diseases included cerebrovascular and neurological diseases (56.7%), osteoarticular diseases (45.3%), diabetes mellitus (25.7%), cardiovascular diseases (25.5%), and chronic respiratory diseases. The study included a home care intervention by the MPRT and PCCMNs and included the following main outcome measures: age, sex, main caregiver, disabling process (ICD-9), type and number of inclusion categories for chronic disease, initial and final Barthel index (BI), treatment or intervention on the patient (techniques), objectives, and number of sessions. The main techniques used were kinesiotherapy (44.6%) and caregiver training (23%), along with technical aid. An equation predicting the patients’ final BI, according to the initial BI, was constructed using multiple linear regression modelling. A marked improvement in functional capacity was found after an average of 10 physiotherapy sessions. A lower patient age was correlated with a higher functional capacity, both initial and final BI, as well as a greater number of sessions.
Age Group Differences in Household Accident Risk Perceptions and Intentions to Reduce Hazards
Authors: Morgan James , Reidy John , Probst Tahira
Published: June 2019

Very little is known about the extent to which seemingly vulnerable younger and older adults appraise household risks and relatedly whether safety information focused on raising risk awareness influences intentions to reduce hazards in the home. The present study assessed age differences in accident experience, risk attitudes, household accident risk perceptions, comparative optimism, personal control, efficacy judgements, and intentions to remove household hazards. It also examined the predictors of these intentions. Thirty-eight younger adults (aged 18 to 25) and forty older adults (aged 65 to 87) completed study booklets containing all measures. There were significant age group differences for all accident experience and risk-related variables. Younger adults experienced more accidents, had riskier attitudes, and had significantly lower cognitive risk perceptions (i.e., they were less likely to be injured due to a household accident). They also had lower affective risk perceptions (i.e., they were less worried) about their accident risk and perceived more personal control over the risk compared with older adults. Young adults were comparatively optimistic about their risk while older adults were pessimistic. Older adults had higher response efficacy and intentions to reduce hazards in the home. Only worry, response efficacy, and risk attitudes predicted intention, however, these relationships were not moderated by age or efficacy appraisal. Although tentative theoretical and practical implications are presented, further research is required in order to better understand the objective and subjective risk associated with household accidents, and to determine the factors that may improve safety, particularly for those most vulnerable.
Exploring Generational Differences of British Ethnic Minorities in Smoking Behavior, Frequency of Alcohol Consumption, and Dietary Style
Authors: Wang Senhu , Li Shuanglong
Published: June 2019

Background: This article explores ethnic minority generational differences in smoking behavior, frequency of alcohol consumption, and dietary style in Britain, and whether these differences can be explained by generational differences in socioeconomic status and ethnic identity. Method: Multivariate analyses using wave 2 (2010–2012) and wave 5 (2013–2015) of the United Kingdom Household Longitudinal Study on smoking behavior, frequency of alcohol consumption, and dietary style from 59,189 White British, 1690 Indians, 960 Pakistanis, 555 Bangladeshis, 1060 Black Caribbeans, and 1059 Black Africans, adjusted for demographic characteristics, socioeconomic status and ethnic identity. Results: While we find little evidence for generational differences in dietary style, second-generation Indians, Pakistanis, and Black Caribbeans have a significantly higher probability of smoking than the first-generation, and all second-generation minorities are significantly more likely to consume alcohol than their first-generation counterparts. Such generational differences in alcohol consumption are partly explained by second-generation minorities’ weakened ethnic identity and higher socioeconomic status. Conclusions: This study facilitates a better understanding of minority generational differences in health behaviors and the role of socioeconomic status and ethnic identity, highlighting the need for future policy interventions to target certain second-generation ethnic minorities who have adopted certain host society unhealthy lifestyles.
Effects of Lanthanum on the Photosystem II Energy Fluxes and Antioxidant System of Chlorella Vulgaris and Phaeodactylum Tricornutum
Authors: Sun Dong , He Ning , Chen Qi , Duan Shunshan
Published: June 2019

The rare earth elements are widely used in agricultural and light industry development. They promote the growth of crop seedlings, enhance root development and change the metal properties. Due to the large amount of rare earth minerals mined in China, rare earth elements have been detected in both coastal and estuary areas. They cause pollution and threaten the health of aquatic organisms and human beings. This study investigates the effects of lanthanum on two marine bait algae, and analyzes the changes in the photosynthetic and antioxidant systems of the two algae. The results show that rare earth elements have significant inhibitory effects upon the two algae. The OJIP kinetic curve value decreases with an increasing concentration of La(NO3)3 ·6H2O. The parameters of the fluorescence value were analyzed. The ABS/RC increases and the DI0/RC decreases during the first 24 h after exposure. The effects on the photosynthetic and antioxidant systems at low concentrations (both EC10 and EC20) show that the TR0/ABS increases, and the ET0/RC, ABS/RC, and DI0/RC has a decreasing trend after 30 min. However, after 24 h, normal levels were restored. In addition, the study finds that the TR0/ABS increases after 24 h, leading to an increase in reactive oxygen species. The antioxidant system analysis also confirms the increase in the activities of antioxidant enzymes, such as SOD and GSH. The experiment is expected to support the marine pollution of rare earths and the theoretical data of the impact on marine primary producers.
Effectiveness of Enzyme Dentifrices on Oral Health in Orthodontic Patients: A Randomized Controlled Trial
Authors: Cheng Hsin-Chung , Hu Hao-Ting , Chang Ya-Chu
Published: June 2019

Plaque accumulation and white spot lesions are common adverse effects of fixed orthodontic appliance use. This study compared the effects between enzyme-containing and conventional dentifrices on orthodontic patients. This double-blind randomized controlled trial included 42 orthodontic patients (25 women and 17 men: 22.7 ± 4.2 years) from Taipei Medical University Hospital between 2017 and 2018. The patients were randomly divided into three groups and assigned to dentifrice use during the first 3 months of the orthodontic treatment: group 1 used dentifrices containing enzymes including amyloglucosidase and glucose oxidase, group 2 used dentifrices containing 1450 ppm fluoride, and group 3 used natural dentifrices containing no chemical agent. White spot lesion index (WSL), gingival bleeding index (GBI), and visible plaque index (VPI) were recorded and analyzed. WSL, GBI, and VPI values exhibited no significant difference among the three groups. WSL increased significantly in group 3, GBI decreased significantly in all groups, and VPI decreased significantly in groups 1 and 2. No significant difference was observed between the use of enzyme-containing and conventional dentifrices after fixed orthodontic appliance placement.
The Distribution of Available Prevention and Management Interventions for Fetal Alcohol Spectrum Disorder (2007 to 2017): Implications for Collaborative Actions
Authors: Adebiyi Babatope O. , Mukumbang Ferdinand C. , Erasmus Charlene
Published: June 2019

The global prevalence of Fetal Alcohol Spectrum Disorder (FASD) remains high despite the various preventive and management interventions that have been designed and implemented to tackle the issue in various settings. The aim of the scoping review is to identify and classify prevention and management interventions of FASD reported globally across the life span and to map the concentration of these interventions across the globe. We searched some selected databases with predefined terms. Framework and narrative approaches were used to synthesize and report on the findings. Thirty-two prevention intervention studies and 41 management interventions studies were identified. All the interventions were reported to be effective or showed promising outcomes for the prevention and management of FASD, except four. Although Europe and Africa have a relatively higher prevalence of FASD, the lowest number of interventions to address FASD were identified in these regions. Most of the interventions for FASD were reported in North America with comparatively lower FASD prevalence. The uneven distribution of interventions designed for FASD vis-à-vis the burden of FASD in the different regions calls for a concerted effort for knowledge and intervention sharing to enhance the design of contextually sensitive preventive and management policy in the different regions.
The Use of a Quasi-Experimental Study on the Mortality Effect of a Heat Wave Warning System in Korea
Authors: Heo Seulkee , Nori-Sarma Amruta , Lee Kwonsang , Benmarhnia Tarik , Dominici Francesca , Bell Michelle L.
Published: June 2019

Many cities and countries have implemented heat wave warning systems to combat the health effects of extreme heat. Little is known about whether these systems actually reduce heat-related morbidity and mortality. We examined the effectiveness of heat wave alerts and health plans in reducing the mortality risk of heat waves in Korea by utilizing the discrepancy between the alerts and the monitored temperature. A difference-in-differences analysis combined with propensity score weighting was used. Mortality, weather monitoring, and heat wave alert announcement data were collected for 7 major cities during 2009–2014. Results showed evidence of risk reduction among people aged 19–64 without education (−0.144 deaths/1,000,000 people, 95% CI: −0.227, −0.061) and children aged 0–19 (−0.555 deaths/1,000,000 people, 95% CI: −0.993, −0.117). Decreased cardiovascular and respiratory mortality was found in several subgroups including single persons, widowed people, blue-collar workers, people with no education or the highest level of education (university or higher). No evidence was found for decreased all-cause mortality in the population (1.687 deaths/1,000,000 people per day; 95% CI: 1.118, 2.255). In conclusion, heat wave alerts may reduce mortality for several causes and subpopulations of age and socio-economic status. Further work needs to examine the pathways through which the alerts impact subpopulations differently.
Gentrification and Displacement in the San Francisco Bay Area: A Comparison of Measurement Approaches
Authors: Mujahid Mahasin S. , Sohn Elizabeth Kelley , Izenberg Jacob , Gao Xing , Tulier Melody E. , Lee Matthew M. , Yen Irene H.
Published: June 2019

Gentrification may play an important role in influencing health outcomes, but few studies have examined these associations. One major barrier to producing empirical evidence to establish this link is that there is little consensus on how to measure gentrification. To address this barrier, we compared three gentrification classification methodologies in relation to their ability to identify neighborhood gentrification in nine San Francisco Bay Area counties: the Freeman method, the Landis method, and the Urban Displacement Project (UDP) Regional Early Warning System. In the 1580 census tracts, 43% of the population had a bachelor’s degree or higher. The average median household income was $79,671 in 2013. A comparison of gentrification methodologies revealed that the Landis and Freeman methodologies characterized the vast majority of census tracts as stable, and only 5.2% and 6.1% of tracts as gentrifying. UDP characterized 46.7% of tracts at risk, undergoing, or experiencing advanced stages of gentrification and displacement. There was substantial variation in the geographic location of tracts identified as gentrifying across methods. Given the variation in characterizations of gentrification across measures, studies evaluating associations between gentrification and health should consider using multiple measures of gentrification to examine the robustness of the study findings across measures.
Carbonic Anhydrase as a Biomarker of Global and Local Impacts: Insights from Calcifying Animals
Authors: Zebral Yuri Dornelles , da Silva Fonseca Juliana , Marques Joseane Aparecida , Bianchini Adalto
Published: June 2019

The emission of greenhouse gases has grown in unprecedented levels since the beginning of the industrial era. As a result, global climate changes, such as heightened global temperature and ocean acidification, are expected to negatively impact populations. Similarly, industrial and urban unsustainable development are also expected to impose local impacts of their own, such as environmental pollution with organic and inorganic chemicals. As an answer, biomarkers can be used in environmental programs to assess these impacts. These tools are based in the quantification of biochemical and cellular responses of target species that are known to respond in a sensitive and specific way to such stresses. In this context, carbonic anhydrase has shown to be a promising biomarker candidate for the assessment of global and local impacts in biomonitoring programs, especially in marine zones, such as coral reefs, considering the pivotal role of this enzyme in the calcification process. Therefore, the aim of this review is to show the recent advances in the carbonic anhydrase research and the reasons why it can be considered as a promising biomarker to be used for calcifying organisms.
Special Issue on Basic and Translational Research in Colorectal Cancer
Authors: Ulivi Paola , Scarpi Emanuela , Passardi Alessandro
Published: June 2019

The present editorial aims to summarize the 17 scientific papers that have contributed to this Special Issue focusing on different aspects of basic and translational research in colorectal cancer.
Serum Proteome Alterations in Human Cystathionine β-Synthase Deficiency and Ischemic Stroke Subtypes
Authors: Sikora Marta , Lewandowska Izabela , Kupc Małgorzata , Kubalska Jolanta , Graban Ałła , Marczak Łukasz , Kaźmierski Radosław , Jakubowski Hieronim
Published: June 2019

Ischemic stroke induces brain injury via thrombotic or embolic mechanisms involving large or small vessels. Cystathionine β-synthase deficiency (CBS), an inborn error of metabolism, is associated with vascular thromboembolism, the major cause of morbidity and mortality in affected patients. Because thromboembolism involves the brain vasculature in these patients, we hypothesize that CBS deficiency and ischemic stroke have similar molecular phenotypes. We used label-free mass spectrometry for quantification of changes in serum proteomes in CBS-deficient patients (n = 10) and gender/age-matched unaffected controls (n = 14), as well as in patients with cardioembolic (n = 17), large-vessel (n = 26), or lacunar (n = 25) ischemic stroke subtype. In CBS-deficient patients, 40 differentially expressed serum proteins were identified, of which 18 were associated with elevated homocysteine (Hcy) and 22 were Hcy-independent. We also identified Hcy-independent differentially expressed serum proteins in ischemic stroke patients, some of which were unique to a specific subtype: 10 of 32 for cardioembolic vs. large-vessel, six of 33 for cardioembolic vs. lacunar, and six of 23 for large-vessel vs. lacunar. There were significant overlaps between proteins affected by CBS deficiency and ischemic stroke, particularly the cardioembolic subtype, similar to protein overlaps between ischemic stroke subtypes. Top molecular pathways affected by CBS deficiency and ischemic stroke subtypes included acute phase response signaling and coagulation system. Similar molecular networks centering on NFκB were affected by CBS deficiency and stroke subtypes. These findings suggest common mechanisms involved in the pathologies of CBS deficiency and ischemic stroke subtypes.
Age-Associated mRNA and miRNA Expression Changes in the Blood-Brain Barrier
Authors: Goodall Emily F. , Leach Vicki , Wang Chunfang , Cooper-Knock Johnathan , Heath Paul R. , Baker David , Drew David R. , Saffrey M. Jill , Simpson Julie E. , Romero Ignacio A. , Wharton Stephen B.
Published: June 2019

Functional and structural age-associated changes in the blood-brain barrier (BBB) may affect the neurovascular unit and contribute to the onset and progression of age-associated neurodegenerative pathologies, including Alzheimer’s disease. The current study interrogated the RNA profile of the BBB in an ageing human autopsy brain cohort and an ageing mouse model using combined laser capture microdissection and expression profiling. Only 12 overlapping genes were altered in the same direction in the BBB of both ageing human and mouse cohorts. These included genes with roles in regulating vascular tone, tight junction protein expression and cell adhesion, all processes prone to dysregulation with advancing age. Integrated mRNA and miRNA network and pathway enrichment analysis of the datasets identified 15 overlapping miRNAs that showed altered expression. In addition to targeting genes related to DNA binding and/or autophagy, many of the miRNAs identified play a role in age-relevant processes, including BBB dysfunction and regulating the neuroinflammatory response. Future studies have the potential to develop targeted therapeutic approaches against these candidates to prevent vascular dysfunction in the ageing brain.
Exploring the Hospital Microbiome by High-Resolution 16S rRNA Profiling
Authors: Rampelotto Pabulo H. , Sereia Aline F.R. , de Oliveira Luiz Felipe V. , Margis Rogério
Published: June 2019

The aim of this work was to analyze and compare the bacterial communities of 663 samples from a Brazilian hospital by using high-throughput sequencing of the 16S rRNA gene. To increase taxonomic profiling and specificity of 16S-based identification, a strict sequence quality filtering process was applied for the accurate identification of clinically relevant bacterial taxa. Our results indicate that the hospital environment is predominantly inhabited by closely related species. A massive dominance of a few taxa in all taxonomic levels down to the genera was observed, where the ten most abundant genera in each facility represented 64.4% of all observed taxa, with a major predominance of Acinetobacter and Pseudomonas. The presence of several nosocomial pathogens was revealed. Co-occurrence analysis indicated that the present hospital microbial network had low connectedness, forming a clustered topology, but not structured among groups of nodes (i.e., modules). Furthermore, we were able to detect ecologically relevant relationships between specific microbial taxa, in particular, potential competition between pathogens and non-pathogens. Overall, these results provide new insight into different aspects of a hospital microbiome and indicate that 16S rRNA sequencing may serve as a robust one-step tool for microbiological identification and characterization of a wide range of clinically relevant bacterial taxa in hospital settings with a high resolution.
Lithium and Not Acetoacetate Influences the Growth of Cells Treated with Lithium Acetoacetate
Authors: Vidali Silvia , Aminzadeh-Gohari Sepideh , Vatrinet Renaud , Iommarini Luisa , Porcelli Anna Maria , Kofler Barbara , Feichtinger René Günther
Published: June 2019

The ketogenic diet (KD), a high-fat/low-carbohydrate/adequate-protein diet, has been proposed as a treatment for a variety of diseases, including cancer. KD leads to generation of ketone bodies (KBs), predominantly acetoacetate (AcAc) and 3-hydroxy-butyrate, as a result of fatty acid oxidation. Several studies investigated the antiproliferative effects of lithium acetoacetate (LiAcAc) and sodium 3-hydroxybutyrate on cancer cells in vitro. However, a critical point missed in some studies using LiAcAc is that Li ions have pleiotropic effects on cell growth and cell signaling. Thus, we tested whether Li ions per se contribute to the antiproliferative effects of LiAcAc in vitro. Cell proliferation was analyzed on neuroblastoma, renal cell carcinoma, and human embryonic kidney cell lines. Cells were treated for 5 days with 2.5, 5, and 10 mM LiAcAc and with equimolar concentrations of lithium chloride (LiCl) or sodium chloride (NaCl). LiAcAc affected the growth of all cell lines, either negatively or positively. However, the effects of LiAcAc were always similar to those of LiCl. In contrast, NaCl showed no effects, indicating that the Li ion impacts cell proliferation. As Li ions have significant effects on cell growth, it is important for future studies to include sources of Li ions as a control.
MMP-12, Secreted by Pro-Inflammatory Macrophages, Targets Endoglin in Human Macrophages and Endothelial Cells
Authors: Aristorena Mikel , Gallardo-Vara Eunate , Vicen Matej , de Las Casas-Engel Mateo , Ojeda-Fernandez Luisa , Nieto Concepción , Blanco Francisco J. , Valbuena-Diez Ana C. , Botella Luisa M. , Nachtigal Petr , Corbi Angel L. , Colmenares María , Bernabeu Carmelo
Published: June 2019

Upon inflammation, monocyte-derived macrophages (MΦ) infiltrate blood vessels to regulate several processes involved in vascular pathophysiology. However, little is known about the mediators involved. Macrophage polarization is crucial for a fast and efficient initial response (GM-MΦ) and a good resolution (M-MΦ) of the inflammatory process. The functional activity of polarized MΦ is exerted mainly through their secretome, which can target other cell types, including endothelial cells. Endoglin (CD105) is a cell surface receptor expressed by endothelial cells and MΦ that is markedly upregulated in inflammation and critically involved in angiogenesis. In addition, a soluble form of endoglin with anti-angiogenic activity has been described in inflammation-associated pathologies. The aim of this work was to identify components of the MΦ secretome involved in the shedding of soluble endoglin. We find that the GM-MΦ secretome contains metalloprotease 12 (MMP-12), a GM-MΦ specific marker that may account for the anti-angiogenic activity of the GM-MΦ secretome. Cell surface endoglin is present in both GM-MΦ and M-MΦ, but soluble endoglin is only detected in GM-MΦ culture supernatants. Moreover, MMP-12 is responsible for the shedding of soluble endoglin in vitro and in vivo by targeting membrane-bound endoglin in both MΦ and endothelial cells. These data demonstrate a direct correlation between GM-MΦ polarization, MMP-12, and soluble endoglin expression and function. By targeting endothelial cells, MMP-12 may represent a novel mediator involved in vascular homeostasis.
High-Fish Oil and High-Lard Diets Differently Affect Testicular Antioxidant Defense and Mitochondrial Fusion/Fission Balance in Male Wistar Rats: Potential Protective Effect of ω3 Polyunsaturated Fatty Acids Targeting Mitochondria Dynamics
Authors: Migliaccio Vincenzo , Sica Raffaella , Di Gregorio Ilaria , Putti Rosalba , Lionetti Lillà
Published: June 2019

High-fat diets rich in fish oil (HFO diet, mainly ω3-PUFAs), in contrast to high-fat diets rich in lard (HL diet, mainly saturated fatty acids) have been shown to induce improvement in mitochondrial function and fusion processes associated with a reduction in reactive oxygen species production in both liver and skeletal muscle. High-fat diets may also impair testicular function, and mitochondria represent important cellular organelles with a pivotal role in reproductive function. Mitochondria are dynamic organelles that frequently undergo fission/fusion processes. A shift toward mitochondrial fusion process has been associated with improvement of mitochondrial function, as well as with ω3-PUFAs protective effects. The present study aimed to analyze the effect of chronic overfeeding (six weeks) with HFO or HL diet on testicular tissue histology, oxidative stress, antioxidant defenses, and mitochondrial fusion (mitofusin 2) and fission (dynamic related protein 1) protein. Our results showed that HFO diet induced less testicular histology impairment, oxidative stress, and apoptosis compared to a HL diet. This finding was associated with an increase in antioxidant activities and a shift toward mitochondrial fusion processes induced by HFO diet compared to HL diet, suggesting that ω3-PUFAs may act as bioactive compound targeting mitochondria dynamics to prevent testicular impairment.
Effects of Estrogens on Platelets and Megakaryocytes
Authors: Dupuis Marion , Severin Sonia , Noirrit-Esclassan Emmanuelle , Arnal Jean-François , Payrastre Bernard , Valéra Marie-Cécile
Published: June 2019

In women, oral menopausal hormonal therapy (MHT) is associated with adverse effects including an increased incidence of thromboembolic events, classically attributed to an increase in several liver-derived coagulation factors due to hepatic first pass. While platelets are central players in thrombus constitution, their implication in women treated with estrogens remains incompletely characterized. Platelets and their medullar progenitors, megakaryocytes, express estrogen receptors (ER) that may explain, at least in part, a sensitivity to hormonal changes. The purpose of this review is to summarize our current knowledge of estrogen actions on platelets and megakaryocytes in mice following in vivo administration and in women using MHT.
Using an Exponential Random Graph Model to Recommend Academic Collaborators
Authors: Al-Ballaa Hailah , Al-Dossari Hmood , Chikh Azeddine
Published: June 2019

Academic collaboration networks can be formed by grouping different faculty members into a single group. Grouping these faculty members together is a complex process that involves searching multiple web pages in order to collect and analyze information, and establishing new connections among prospective collaborators. A recommender system (RS) for academic collaborations can help reduce the time and effort required to establish a new collaboration. Content-based recommendation system make recommendations based on similarity without taking social context into consideration. Hybrid recommender systems can be used to combine similarity and social context. In this paper, we propose a weighting method that can be used to combine two or more social context factors in a recommendation engine that leverages an exponential random graph model (ERGM) based on historical network data. We demonstrate our approach using real data from collaborations with faculty members at the College of Computer and Information Sciences (CCIS) in Saudi Arabia. Our results demonstrate that weighting social context factors helps increase recommendation accuracy for new users.
Promoting Physical Exercise Participation: The Role of Interpersonal Behaviors for Practical Implications
Authors: Rodrigues Filipe , Teixeira Diogo S. , Cid Luís , Monteiro Diogo
Published: June 2019

The number of people engaging in physical exercise has been decreasing every year. These behaviors are known to be related with non-communicable chronic diseases and to drastically increase premature morbidity and mortality. Since “the lack of motivation” has been pointed out as one of the main reasons for not engaging in physical exercise, several theoretical and empirical studies have been conducted aimed at understanding what influences behavior regulation. According to literature, gym exercisers who perceive exercise instructors as supportive are more likely to maintain physical exercise participation over the long-run. Supporting autonomy, competence, and relatedness should be carefully considered when interacting with health club clients as a way to promote more autonomous motivation. Overall, it seems that exercise instructors should foster a supportive environment for gym exercisers, in order to encourage exercise as a habitual behavior.
Motion Control for Autonomous Navigation in Blue and Narrow Waters Using Switched Controllers
Authors: Alessandri Angelo , Donnarumma Silvia , Martelli Michele , Vignolo Stefano
Published: June 2019

Autonomous ships represent one of the new frontiers of technological innovation in marine engineering, which demand the development of innovative control systems to guarantee efficient and safe navigation of vessels. A convenient control system should be able to command the several actuators installed on board in different conditions—for instance, during oceanic navigation, harbor approach, narrow channels, and crowed areas. Such tasks are accomplished by different switching controllers for high and low speed motion, which have to be orchestrated to ensure an effective maneuvering. An approach to the design of hierarchies of controllers for maneuvering and navigation of ships equipped with a standard propulsion configuration in both blue and narrow water is proposed. Different levels of control, from global to local, are defined and integrated to steer the vessel in such a way to increase the maneuvering capability in various scenarios.
Synthesis and Crystal Structure of A Pyrithione Derivative: Bis{2-[(1-oxidopyridin-2-yl)sulfanyl]-4,5-dihydro-1H-imidazol-3-ium} tetrachlorocuprate(2-)
Authors: Balewski Łukasz , Sączewski Franciszek , Gdaniec Maria
Published: June 2019

The pyrithione derivative, bis{2-[(1-oxidopyridin-2-yl)sulfanyl]-4,5-dihydro-1H-imidazol-3-ium} tetrachlorocuprate(2-) (1a) has been obtained by the reaction of one equivalent of 2-[(4,5-dihydro-1H-imidazol-2-yl)thio]pyridine 1-oxide hydrochloride with one and a half equivalents of copper (II) chloride dihydrate in methanol in a very good yield. The structure of this product was confirmed by X-ray crystallography, infrared spectroscopy, and elemental analysis.
Essential Oil Microcapsules Immobilized on Textiles and Certain Induced Effects
Authors: Stan Miruna S. , Chirila Laura , Popescu Alina , Radulescu Denisa M. , Radulescu Diana E. , Dinischiotu Anca
Published: June 2019

In order to obtain textile materials with potential utility in the development of cosmetic textiles, this study examined the deposition by padding of rose and sage microcapsules on woven textile structures, with different fiber compositions (100% cotton and 50% cotton/50% polyester). Cationization of the textile materials was performed to enhance the degree of uptake the pf the microcapsules on the fabrics’ surface. A commercially acrylate-based binder was used to fix the microcapsules to the textile substrate and to improve the durability against external factors. The finished textile materials were characterized in terms of their physical-mechanical characteristics. The distribution of microcapsules on the fabrics surface before and after five washing cycles and 1000 abrasion cycles was investigated by scanning electron microscopy. The biocompatibility in terms of cell viability, cell membrane integrity and inflammation status of the functionalized fabrics was evaluated on CCD-1070Sk normal human dermal fibroblasts. The cell morphology was evaluated by F-actin staining using fluorescence microscopy and no significant changes were noticed after the incubation in the presence of fabrics compared with control. The in vitro biocompatibility evaluation on human skin cells confirmed the absence of cytotoxicity after the short-term exposure, supporting further in vivo use of these innovative textiles with improved properties.
High Sulfate Attack Resistance of Reinforced Concrete Flumes Containing Liquid Crystal Display (LCD) Waste Glass Powder
Authors: Kim Seong-Kyum , Hong Won-Kee
Published: June 2019

To prevent chemical erosion of concrete and improve chemical resistance, reinforced concrete flumes were manufactured, conforming to the Korean Industrial Standards (KS). Two different sizes of liquid crystal display (LCD) waste glass powder (LWGP) particles were used (i.e., 5 and 12 µm) with two substitution types with cement in concrete (i.e., 10% and 20%). Changes in compressive strength, pore structure, weight, volume, and strength of the concrete flumes after immersion in two sulfate solutions (i.e., Na2SO4 and MgSO4) for 84 and 182 days were measured for sulfate attack resistance. The applicability of the LWGP concrete flume with a 0.5 mm crack width was also evaluated based on the bending strength results. The LWGP5, which has a smaller particle size among LWGPs, filled the smaller pores, thereby reducing the porosity and contributing to the compressive strength gain. Higher volume and weight change ratios for all specimens immersed in MgSO4 solution were found than those immersed in Na2SO4 solution under identical conditions. Flexural loads of all the LWGP concrete flumes with 0.05 mm crack widths were greater than 48.5 kN, as required by the KS code; however, these flexural loads were lower than those of ordinary Portland cement. The applicability was also validated via a flexural test complying with KS.
Machinability of Rene 65 Superalloy
Authors: Olufayo Oluwole A. , Che Hanqing , Songmene Victor , Katsari Christina , Yue Stephen
Published: June 2019

Nickel-based superalloys are heavily used in the aerospace and power industries due to their excellent material and mechanical properties. They offer high strength at elevated temperatures, high hardness, corrosion resistance, thermal stability and improved fatigue properties. These superalloys were developed to address the demand for materials with the enhanced heat and stress capabilities needed to increase operational temperatures and speeds in jet and turbine engines. However, most of these properties come with machining difficulty, high wear rate, increased force and poor surface finish. Rene 65 is one of the next generation wrought nickel superalloys that addresses these demands at a reduced cost versus powder metallurgy superalloys. It is strengthened by the presence of gamma prime precipitates in its microstructure, which enhance its strength at high temperatures. Notwithstanding its advantages, Rene 65 must also deal with the reality of the poor workability and machinability generally associated with Ni-based superalloys. This study examines the machinability—using drilling tests—of Rene 65 and seeks to establish the influence of hardness (with varying microstructure) and cutting conditions on machinability indicators (surface finish, forces and chip formation). The experimental setup is based on a set of experimental drilling tests using three different heat-treated samples of varying hardness. The results indicate a negligible effect from material hardness, ranging from 41 HRC to 52 HRC, on generated cutting forces and a similarly low effect from cutting speeds. The feed rate was identified as the main factor of relevance in cutting force and chip morphology during the machining of this new superalloy.