bluuebunny/crossref_metadata_2025 huggingface dataset:
A Descriptive Exploration of Heart Rate Response to Live Professional Irish Dance Performance
Authors: Needham-Beck Sarah C. , Smith Marcus S. , West Andy T. , Quin Edel
Published: July 2024

Introduction: Research investigating the physiological demands of dance performance has documented exercise intensity and time spent in work and rest; however, available data are across limited dance genres and often use rehearsal or simulated conditions. Real-time data recorded during live performance with an audience are limited. The aim of the study is to provide a detailed description of the physiological demands of live professional Irish dance performance. Method: The entire Irish dance cast of Riverdance participated in the study (n = 24). Approval was granted by the Institutional Research Ethics Committee. Matinee and evening performances on the first day of a tour were examined in a descriptive, cross-sectional design. Heart rate (HR) monitors worn discretely under costumes recorded data every 5-seconds. Video analysis allowed calculation of time spent in work and rest. All descriptive analyses were split by role (troupe/lead) and sex (male/female). Results: Dancers worked at hard/very hard intensities (mean > 72%, peak > 91% HRmax) for ~3 minutes at a time for 3-9 individual dance numbers. There was a high variation in overall demand experienced by individual dancers due to variation in both duration and intensity of on-stage time, dependent upon their role. Mean total on-stage time was 18.18 ± 5.82 minutes, 19.43 ± 5.80 minutes, 29.71 ± 0.62 minutes, 20.00 ± 0.00 minutes for male troupe, female troupe, male lead, and female lead dancers, respectively. Recovery periods were varied in terms of duration, activity undertaken, and HR response. Conclusion: Individual dance numbers within a professional Irish dance show were high intensity for a short duration when considered in isolation, but overall physiological demand experienced by any one dancer across the entire show varied dependent upon their role and off-stage/recovery practices. Future research investigating the physiological demands of dance performance should ensure analysis accounts for the individual dancer’s role and should capture the entire show duration in repeated exposures.
Leukocyte-Rich Platelet-Rich Plasma for Osteonecrosis of Sesamoid in a Performing Artist: A Case Report
Authors: Callahan Elizabeth A. , Peyser Kenneth , Espiritu-McKay Tracy
Published: July 2024

Introduction: Sesamoid bones absorb and disperse weight from the metatarsal bones. Sesamoid injuries occur primarily in dancers and runners, with nonoperative management as the first line of treatment. Due to the lack of secondary blood supply, the sesamoids are at risk for osteonecrosis and nonunion fractures. The literature supporting orthobiologics for the treatment of sesamoid injuries is limited. Methods: In this case, a 28-year-old female dancer with sesamoid osteonecrosis underwent a leukocyte-rich platelet-rich plasma (LR-PRP) injection to the fibular sesamoid. Results: The patient reported significant improvement in pain at the 2-month follow-up and returned to regular auditions at the 3-month follow-up. At 2-year follow-up, the patient’s symptoms returned to baseline. Conclusion: This is the first known case of sesamoid osteonecrosis treated with LR-PRP. LR-PRP should be considered in the treatment of sesamoid osteonecrosis in those who show limited improvement with standard conservative measures.
When do member state withdrawals lead to the death of international organizations?
Authors: von Borzyskowski Inken , Vabulas Felicity
Published: July 2024

Recent research has drawn attention to states’ backlash against international organizations (IOs), including whether member state withdrawals affect the longevity of IOs. We therefore ask when do member state withdrawals lead to the death of IOs? We are skeptical of a general link between withdrawal and IO death because on average, any one member is not critical for the survival of an IO. Also, withdrawal is often driven by one member state’s preferences diverging from remaining members; these remaining states may band together after withdrawal, ensuring or even enhancing the longevity of the IO. Even withdrawal by several states may not contribute to IO death because a smaller group of remaining members may better overcome collective action challenges. Nonetheless, exit by an important member may affect IO survival by removing resources, market power, and guidance. We test these arguments using survival models on an original dataset of withdrawals across 532 IOs from 1909 to 2014/2020 and illustrate the dynamics with case vignettes. The results support our arguments: withdrawals in general do not lead to IO death but the withdrawal of founding members can speed IO death. Interestingly, withdrawal by economically powerful states seems to facilitate IO survival (often through reform and/or re-entry). These findings contribute to a better understanding of the lifecycle of IOs as well as to the resilience and vulnerabilities of international cooperation.
Engine-out hydrocarbon speciation and DOC reaction modeling for dual-fuel combustion concept
Authors: García Antonio , Pastor José V , Monsalve-Serrano Javier , Fogué-Robles Álvaro
Published: July 2024

The concerns for global warming have pushed very harsh regulations on conventional propulsion systems based on the use of fossil fuels. New technologies are being promoted, but their current technological status needs further research and development to become a competitive substitute for the ever-present internal combustion engine. Transition technologies like hybrid-electric platforms are the preferred solution, but their dependence on the internal combustion engine demands continued developing and improving this technology. Advanced combustion modes like dual-mode dual-fuel combustion are attractive solutions with room for improvement. This work evaluates the specifics of the hydrocarbon composition emitted during the operation of a medium-duty dual-mode dual-fuel engine, analyzing the specific requirements of a diesel oxidation catalyst for this application. Also, the modeling approach of this after-treatment component is revised for this type of application, proposing a new approach and evaluating numerically the performance of a conventional diesel oxidation catalyst. The results show that the new modeling approach brings better accuracy when modeling the transient operation of the engine.
Monitoring and assessing concrete member states using implantable sensing technology and enhanced long short-term memory networks
Authors: Kong Qingzhao , Ding Yewei , Ma Bin , Qin Xiaoming , Yang Ziqian
Published: July 2024

Monitoring the state of concrete structures and assessing their performance are significant tasks for civil engineering. This study proposes a combined technique of novel concrete implantable bar (CIB) transducers and enhanced long short-term memory (LSTM) networks for monitoring and assessing reinforced concrete (RC) beam state over the whole loading process. The CIB can be installed on the inspected structure in an implantable manner. It contains an array of piezoceramic sensing units that can generate and receive ultrasonic waves from a concrete medium. To enhance the LSTM network’s ability to learn very long-series data, a time-shift energy (TSE) strategy was developed. Compared with another existing convolutional neural network (CNN)–LSTM network, the proposed TSE–LSTM network is highlighted to fully consider the ultrasound propagation characteristics in concrete when extracting sample features, instead of using simple convolution operation. A numerical study was conducted to investigate the sensitivity of the TSE feature to different concrete damage levels through mesoscale finite element models. The results provided the best parameter settings of the TSE. Eventually, to validate the feasibility of the proposed technique, an RC beam four-point bending test was carried out, in which two CIBs were implanted into the specimen for emitting and collecting ultrasonic waves in different damage states to establish a dataset. Two schemes including a classification model for predicting RC beam stress stages and another regression model for predicting the carried forces were separately investigated. The experimental results showed that the TSE–LSTM networks can successfully predict the signature of three stages of RC beams and can, in general, predict their carried forces. The comparison to the results obtained by CNN–LSTM networks further highlighted the stability and accuracy of the proposed one in learning long ultrasound series. The combined technique of CIB transducers and TSE–LSTM networks shows a promising application for monitoring and assessing RC structures.
The Promise and Practicality of Addressing Sex as a Biological Variable and the Ovarian Cycle in Preclinical Epilepsy Research
Authors: Christian-Hinman Catherine A.
Published: July 2024

Seizures and epilepsy affect people of all sexes and genders. In the last several years, funding agency initiatives such as the U.S. National Institutes of Health policy on sex as a biological variable (SABV) have intended to encourage researchers to study both males and females from cell to tissue to organism and analyze and report the resulting data with sex as a factor. Preclinical epilepsy research, however, continues to be plagued by confusion regarding both the SABV policy and its implementation, reflecting similar beliefs in the larger neuroscience research community. This article aims to address some common misconceptions and provide practical tools and suggestions for preclinical epilepsy researchers in implementing SABV and analysis of the female ovarian cycle (estrous cycle in rodents) in their research programs, with a focus on studies using rodent models. Examples of recent publications in preclinical epilepsy research highlighting the value of incorporating SABV and information on the estrous cycle are included. The specifics of how best to address SABV and the estrous cycle can vary depending on the needs and goals of a particular research program, but an embrace of these physiological factors by the preclinical epilepsy research community promises to yield more rigorous research and improved treatment strategies for all people with epilepsy.
Numerical study on the effect of the stenters’ nozzle structure on fabric drying rate and surface heat-moisture distribution characteristics
Authors: Dai Jia-ao , Diao Yong fa , Zhang Lei
Published: July 2024

Stenters play a crucial role in the post-treatment of fabric dyeing and finishing, which is mainly used for drying and heat setting. However, the influence of the orifice structure on the heat-moisture distribution characteristics of the fabric surface is not clear when the air duct sprays high-speed airflow. In this paper, the drying rate of fabrics under four different orifice types (circular orifice, elliptical orifice, square orifice, and slits orifice) are analyzed using numerical method, then the distribution characteristics of heat and moisture on the fabric surface are explored by accurately describing the gas-liquid conversion process. The results show that the elliptical orifice jet has the best heat-moisture distribution on the fabric surface and has a moderate drying rate compared to other orifice types, the decrease of jet height and the increase of orifice size will lead to the increase of fabric drying rate, the thermal action distance of the control region of elliptical orifice jet is obtained. Furthermore, multiple nonlinear methods were used to conduct regression analysis on the changes in fabric moisture content under the influence of the above factors. The numerical results have been verified by experiments, and the relative deviation is within 10%. The research is important for optimizing the air supply system of stenters and improving fabric product quality.
Walkable Maps and Policy Innovation for Nature: A Novel Methodology for Understanding Policy Learning
Authors: Petersen Carolyn J. , Russel Duncan J. , Jensen Anne , Pedersen Anders Branth , Banzhaf Ellen , Kaltenegger Ingrid
Published: July 2024

Methodological innovation is needed that actively engages a range of policy makers in policy learning to address the climate and biodiversity crises. We developed Walkable Floor Map Policy Workshops (WFMPWs) as a way of engaging policy makers in policy learning towards NBS innovation in their local context. This paper examines WFMPW methodology for NBS through an analysis of three WFMPWs in three European urban case study sites. We find that implementation of WFMPWs facilitated policy learning through group discussion and experience sharing amongst participating policy makers. The WFMPWs added greater spatial contextualisation and the futures workshop elements facilitated discussions of future opportunities and barriers, leading to questioning of common approaches and assumptions and ‘thinking outside of the box’. The data provided in-depth, comparative, nuanced, and locally contextualised qualitative insights of interest across multiple disciplines. The findings provide a different way of understanding political and decision-making processes around NBS, including problem framing and shifting policy debates. The WFMPW approach enabled participating policy makers to describe and critique the current situation; imagine/visualise a preferable future situation; and explore ways of moving from the actual situation to the preferred one.
Protective Effect ofChikusetsu saponin IVaon Hydrogen Peroxide Induced Injury of IPEC-J2 Cells
Authors: Liu Qing , Ma Yingfeng , Fan Hanxiao , Li Jialu , Tang Jinbao , Wang Tianjie , Fan Ying , Su Jianming , Lei Hongyu
Published: July 2024

Objectives: Chikusetsu saponin IVa (CHS-IVa) is a saponin compound widely found in herbs such as Panax japonicus C.A.Mey, which has various functions. The purpose of this study was to investigate the protective effect of CHS-IVa on oxidative stress and inflammation in porcine intestinal epithelial cells (IPEC-J2) under the action of hydrogen peroxide (H2O2). Methods: Cell viability was detected by MTT method. The cell scratch experiment was used to test the repair ability of CHS-IVa on cells. The levels of lactate dehydrogenase (LDH), catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), thioredoxin reductase (TrxR), and malondialdehyde (MDA) were tested using the relevant kits. The expression levels of TNF-α, NF-κB, IL-6, IL-1α, and P65 were detected by qPCR. Results: MTT results showed that CHS-IVa at 50 to 100 μg/mL could enhance the activity of IPEC-J2 cells within 24 hours. CHS-IVa at 80 and 100 μg/mL could promote damage repair and effectively inhibit the release of LDH production in IPEC-J2 cells after exposure to H2O2. Additionally, it had a restorative effect on the loss of TrxR, SOD, GSH, and CAT. At the same time, the treatment of CHS-IVa significantly decreased the mRNA expression of NF-κB, IL-6, and P65 after H2O2treatment. Conclusion: These findings indicate that a specific concentration of CHS-IVa has a protective effect on IPEC during oxidative stress, thereby enhancing their ability to repair damage.
Advising Leaders’ Perceptions of Advisor Workplace Learning
Authors: McGill Craig M.
Published: July 2024

This study explores the perceptions of academic advising leaders regarding workplace learning for primary-role academic advisors in the United States. This research highlights the importance of self-directed learning, scholarly engagement, and graduate programs in the professional development of academic advisors. Through semi-structured interviews with 17 leaders of NACADA: The Global Community for Academic Advising, two themes emerged: self-directed learning and engagement, and scholarly engagement and understanding the field. The findings reveal that academic advisors, as proactive learners, continuously enhance their competencies through formal and informal learning activities, actively engaging with academic literature and professional networks. The study underscores the significance of preparing advisors to contribute meaningfully to their profession. By addressing the challenges and opportunities in workplace learning, this research contributes to the evolution and professionalization of academic advising, aligning with principles of Human Resource Development (HRD). The insights gained inform the design of effective professional development programs that enhance advisor skills and knowledge, ultimately leading to improved student outcomes and the advancement of the advising profession. This study bridges a critical gap in understanding the role of workplace learning in the continuous professional growth of academic advisors.
Sublingual asenapine for agitation in malabsorptive states: three patient cases
Authors: Burk Bradley G. , Humphreys Kyle , Waites Jim , Adams Bentley , Birur Badari , Parker Pamela E.
Published: July 2024

Gastric malabsorptive conditions may prevent patients from deriving benefit from orally administered medications intended for enteric absorption. While malabsorption is an increasingly common issue, current data on alternative oral options for agitation in these patients are very sparse. Sublingual (SL) asenapine is absorbed transmucosally, bypassing gut absorption, making it a viable consideration. We report on three patients, one with short bowel syndrome, one with viral gastritis, and one with aortic dissection who were trialed on SL asenapine for agitation after failing alternative antipsychotics. Two of these patients had an extensive history of psychiatric admissions for bipolar disorder and substance-induced psychosis. All three patients had significant reductions in agitation within 1–5 days, with no reported adverse effects. However, benefit of SL asenapine was hindered in two of these patients as they began inappropriately swallowing the medication, reducing bioavailability to nil. Clinicians should consider the use of SL asenapine for medically complex agitated patients where gastric absorption is questionable. There is an urgent need for guidelines on this matter, as well as more, alternative dosage forms for various medications that may help with agitation in this population.
Exploring the barriers and facilitators experienced by patients with heart failure when using popular exergaming platforms for self-management—a systematic review
Authors: Ravani Elham , Ali Fai , Albuainain Sara , Flamarzi Sara , Dirar Tuqa , O’Brien Michelle , Morris Linzette
Published: July 2024

Objective

To systematically explore the barriers and facilitators experienced by patients with heart failure when using the most popular commercially-accessible digital exergaming platforms for self-management.

Methods

A systematic literature search was conducted in: Embase, SCOPUS, PubMed, and ProQuest. Qualitative/mixed methods studies published in English between 2000 and 2024, including adults >18 years with heart failure using digital gaming platforms for exercise as self-management (i.e. Microsoft Xbox/Kinect, Sony PlayStation®, Nintendo™ Wii) were considered. Risk of bias was assessed using the Critical Appraisal Skills Program. The grounded theory method was used to extract, analyze, and synthesize the data.

Results

Three articles which qualitatively reported on the experiences of the patients with heart failure when using Nintendo™ Wii for self-management were included. Seventy-nine participants (male and female, age 32–81 years) were included in the studies. The three included studies were of high quality. Extracted qualitative data were grouped into subthemes, which were grouped into main themes, namely, Engagement, A pathy, Convenience, Health-related benefits and Gaming Platforms. The main themes were categorized into Barriers and Facilitators. Patients reported that gaming platforms were simple, easy to use, interesting, and motivating ( Facilitators). Boredom while playing specific games, preferring other activities and technical issues were reported as Barriers.

Conclusion

This review provides the first insights into the barriers and facilitators patients with heart failure experience when using popular digital exergaming platforms to participate in exercise programs in the self-management of their condition. However, few high-quality studies exist which limits the conclusions made and warrants further research in this area.
Uncovering genetic mimics in multiple sclerosis: A single-center clinical exome sequencing study
Authors: Mandler Julia M. , Härtl Johanna , Cordts Isabell , Sturm Marc , Hedderich Dennis M. , Bafligil Cemsel , Baki Enayatullah , Becker Benedikt , Machetanz Gerrit , Haack Tobias B. , Berthele Achim , Hemmer Bernhard , Deschauer Marcus
Published: July 2024

Background

Multiple sclerosis (MS) shares clinical/radiological features with several monogenic diseases that can mimic MS.

Objective

We aimed to determine if exome sequencing can identify monogenic diseases in patients diagnosed with MS according to the McDonald criteria thus uncovering them as being misdiagnosed.

Methods

We performed whole exome sequencing in a cohort of 278 patients with MS, clinically or radiologically isolated syndrome without cerebrospinal fluid-specific oligoclonal bands (CSF-OCBs) (n = 228), a positive family history of MS (n = 44), or both (n = 6), thereby focusing on individuals potentially more likely to have underlying monogenic conditions mimicking MS. We prioritized 495 genes associated with monogenic diseases sharing features with MS.

Results

A disease-causing variant in NOTCH3 was identified in one patient without CSF-OCBs, no spinal lesions, with non-response to immunotherapy, and a family history of dementia, thereby converting the diagnosis to cerebral autosomal-dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). Moreover, 18 patients (6.5% of total) carried variants of unclear significance.

Conclusion

Monogenic diseases being misdiagnosed as MS seem rare in patients diagnosed with MS according to the McDonald criteria, even in CSF-OCB negative cases. The detected pathogenic NOTCH3 variant emphasizes CADASIL as a rare differential diagnosis and highlights the relevance of genetic testing in selected MS cases with atypical presentations.
Influences of 2-ethylanthraquinone on lignin degradation in alkaline cooking of Eucalyptus pellita wood
Authors: Luo Ke , Nishimoto Taiki , Matsushita Yasuyuki
Published: July 2024

The impact of 2-ethylanthraquinone (2-EAQ) on alkaline cooking of Eucalyptus pellita wood was examined by analyzing pulp yield, kappa number, the structure and weight-averaged molecular weight (Mw) of dissolved lignin in black liquor and residual lignin in pulp (RL). During soda cooking under 23% of active alkaline (AA) condition, adding 0.06% 2-EAQ reduced the kappa number by 12 points compared to cooking without it. The addition of 0.06% 2-EAQ to kraft cooking increased pulp yield by about 4% at about 19 of kappa number compared to the no addition. The analysis of lignin structure by using semiquantitative heteronuclear single quantum correlation nuclear magnetic resonance (HSQC NMR) showed that the addition of 2-EAQ promoted β-ether cleavage during alkaline cooking and reduced peeling reaction of polysaccharides and prevention of the elimination of γ-OH of lignin to form enol ethers and stilbenes. Overall, the addition of 2-EAQ promoted the degradation of lignin to lower molecular weight compounds, with a more pronounced effect observed in soda cooking than in kraft cooking. Under optimal conditions, the delignification efficiency and pulp yield of soda-EAQ pulping were comparable to kraft cooking.
Characteristics of the phenotypes in prevalent and incident cases of heart failure in primary care: IBERICAN study
Authors: Cinza-Sanjurjo Sergio , Prieto-Díaz Miguel Ángel , Pallarés-Carratalá Vicente , Micó-Pérez Rafael M. , Velilla-Zancada Sonsoles , Barquilla-García Alfonso , Ginel-Mendoza Leovigildo , Segura-Fragoso Antonio , Martín-Sánchez Vicente , Polo-García José
Published: July 2024

Background

The management in primary care (PC) of the patients with Heart Failure (HF) is different from the management hospital, in a special way compared to cardiology departments.

Objective

To define the characteristics in both phenotypes of HF in prevalent and incident cases of HF in patients recruited in a large PC sample.

Methods

We proposed a and longitudinal analyses, in patients of the IBERICAN cohort, that recruited 8,066 patients in the Spanish primary care system, with 15,488 patients-years of follow-up. Of them, 252 patients (3.1%) had diagnoses of HF. HF was classified according to the 2014 guidelines in two groups: HF with a reduced eject fraction or HFrEF (LVEF < 50%) and HF with preserved eject fraction or HFpEF (LVEF ≥ 50%). Recommended treatment was defined as the patient receiving drug treatment with Renin-Angiotensin-System (RAS) blockers with beta-blockers and, optionally, spironolactone. The incidence of new cases of HF was calculated in the 7,814 patients without HF in the inclusion visit. Finally, we analysed which variables associated the onset new cases and get the hazard ratio (HR) with the confidence interval at 95% ([95%CI]). Clinical trials register: NCT02261441 (02/05/2017).

Results

The HFpEF was the most frequent phenotype in prevalent cases (61.1%) and incident cases (73.9%). Patients with HFrEF had a higher prevalence of coronary heart disease (p = 0.008) and PAD (p = 0.028), and no statistically significant differences was observed in the therapeutic groups used between both groups. The incidence of HF was 12.8 cases/1000 inhabitants/year, 35.6% of them was diagnosed in PC. The renin-angiotensin system blockers were more used in PC (60%) and beta-blockers (100%) and spironolactone (60%) in hospital. The female sex showed a protective effect for incident cases (0.51 [0.28–0.92]); and AF (HR [95%CI]: 2.90 [1.51–5.54]), coronary heart disease (HR [95%CI]: 2.18 [1.19-4.00]) and hypertension (HR [95%CI]: 1.91 [1.00-3.64]) increased the risk of developing HF.

Conclusions

HF phenotype more frequent and incident in PC was the HFpEF, but only one third of them are diagnosed in PC level. The female sex showed a protective effect and atrial fibrillation, ischaemic heart disease and hypertension increased the risk of develop HF.
GWAS-based polygenic risk scoring for predicting cerebral artery dissection in the Chinese population
Authors: Zhang Shufan , Zhu Dongliang , Wu Zhengyu , Yang Shilin , Liu Yuanzeng , Kang Xiaocui , Chen Xingdong , Zhu Zhu , Dong Qiang , Suo Chen , Han Xiang
Published: July 2024

Objective

Cerebral artery dissection (CeAD) is a rare but serious disease. Genetic risk assessment for CeAD is lacking in Chinese population. We performed genome-wide association study (GWAS) and computed polygenic risk score (PRS) to explore genetic susceptibility factors and prediction model of CeAD based on patients in Huashan Hospital.

Methods

A total of 210 CeAD patients and 280 controls were enrolled from June 2017 to September 2022 in Department of Neurology, Huashan Hospital, Fudan University. We performed GWAS to identify genetic variants associated with CeAD in 140 CeAD patients and 210 control individuals according to a case and control 1:1.5 design rule in the training dataset, while the other 70 patients with CeAD and 70 controls were used as validation. Then Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) enrichment analyses were utilized to identify the significant pathways. We constructed a PRS by capturing all independent GWAS SNPs in the analysis and explored the predictivity of PRS, age, and sex for CeAD.

Results

Through GWAS analysis of the 140 cases and 210 controls in the training dataset, we identified 13 leading SNPs associated with CeAD at a genome-wide significance level of P < 5 × 10− 8. Among them, 10 SNPs were annotated in or near (in the upstream and downstream regions of ± 500Kb) 10 functional genes. rs34508376 (OR2L13) played a suggestive role in CeAD pathophysiology which was in line with previous observations in aortic aneurysms. The other nine genes were first-time associations in CeAD cases. GO enrichment analyses showed that these 10 genes have known roles in 20 important GO terms clustered into two groups: (1) cellular biological processes (BP); (2) molecular function (MF). We used genome-wide association data to compute PRS including 32 independent SNPs and constructed predictive model for CeAD by using age, sex and PRS as predictors both in training and validation test. The area under curve (AUC) of PRS predictive model for CeAD reached 99% and 95% in the training test and validation test respectively, which were significantly larger than the age and sex models of 83% and 86%.

Conclusions

Our study showed that ten risk loci were associated with CeAD susceptibility, and annotated functional genes had roles in 20 important GO terms clustered into biological process and molecular function. The PRS derived from risk variants was associated with CeAD incidence after adjusting for age and sex both in training test and validation.
The correlations on psychopathology in children self-rating, psychopathology in children as related by their parents and psychopathology in parents self-rating in a Kenyan school setting: towards an inclusive family-centered approach
Authors: Ndetei David M. , Mutiso Victoria , Nyamai Pascalyne , Musyimi Christine
Published: July 2024

Several studies have reported on the association between parental and childhood psychopathologies. Despite this, little is known about the psychopathologies between parents and children in a non-clinical population. We present such a study, the first in a Kenyan setting in an attempt to fill this gap. The objective of this study was to determine the association between self-rating psychopathology in children, parent-rating psychopathology in their children and self-rating psychopathology in parents in a non-clinical population of children attending schools in Kenya. We identified 113 participants, comprising children and their parents in 10 randomly sampled primary schools in South East Kenya. The children completed the Youth Self-Report (YSR) scale and parents completed the Child Behavior Check List (CBCL) on their children and the Adult Self-Reports (ASR) on themselves. These instruments are part of the Achenbach System of Empirically Based Assessment (ASEBA), developed in the USA for a comprehensive approach to assessing adaptation and maladaptive behavior in children and adolescents. There was back and forth translation of the instruments from English to Swahili and the local dialect, Kamba. Every revision of the English translation was sent to the instrument author who sent back comments until the revised version was in sync with the version developed by the author. We used the ASEBA in-built algorithm for scoring to determine cut-off points for problematic and non-problematic behavior. Correlations, linear regression and independent sample t-test were used to explore these associations. The mean age of the children was 12.7. While there was no significant association between child problems as measured by YSR (self-reported) and parent problems as measured by ASR and CBCL in the overall correlations, there was a significant association when examining specific groups (clinical range vs. non-clinical). Moreover, significant association existed between total problems on YSR and ASR internalizing problems (t=-2.3,p = 0.023), with clinical range having a higher mean than the normal range. In addition, a significant relationship (p < 0.05) was found between psychopathology in children as reported by both parents (CBCL) and psychopathology in parents as self-reported (ASR).

Mothers were more likely to report lower syndrome scores of their children as compared to fathers. Our findings indicate discrepancies between children self-rating and parent ratings, suggesting that one cannot manage psychopathology in children without reference to psychopathology in their parents. We suggest broad-based psycho-education to include children and parents to enhance shared awareness of psychopathology and uptake of treatment.
Social, economic, and physical side effects impact PrEP uptake and persistence among transgender women in Peru
Authors: Naz-McLean Sarah , Clark Jesse , Huerta Leyla , Mayer Kenneth H. , Lama Javier R. , Reisner Sari , Perez-Brumer Amaya
Published: July 2024

Introduction

Oral pre-exposure prophylaxis (PrEP) for HIV-1 infection is over 99% effective in protecting against HIV acquisition when used consistently and appropriately. However, PrEP uptake and persistent use remains suboptimal, with a substantial gap in utilization among key populations who could most benefit from PrEP. In Latin America specifically, there is poor understanding of barriers to PrEP uptake and persistence among transgender (trans) women.

Methods

In April-May 2018, we conducted qualitative interviews lasting 25–45 min as part of an end-of-project evaluation of TransPrEP, a pilot RCT that examined the impact of a social network-based peer support intervention on PrEP adherence among trans women in Lima, Peru. Participants in the qualitative evaluation, all adult trans women, included individuals who either (1) screened eligible to participate in the TransPrEP pilot, but opted not to enroll (n = 8), (2) enrolled, but later withdrew (n = 6), (3) were still actively enrolled at the time of interview and/or successfully completed the study (n = 16), or (4) were study staff (n = 4). Interviews were audio recorded and transcribed verbatim. Codebook development followed an immersion/crystallization approach, and coding was completed using Dedoose.

Results

Evaluation participants had a mean age of 28.2 years (range 19–47). When describing experiences taking PrEP, participant narratives highlighted side effects that spanned three domains: physical side effects, such as prolonged symptoms of gastrointestinal distress or somnolence; economic challenges, including lost income due to inability to work; and social concerns, including interpersonal conflicts due to HIV-related stigma. Participants described PrEP use within a broader context of social and economic marginalization, with a focus on daily survival, and how PrEP side effects negatively contributed to these stressors. Persistence was, in some cases, supported through the intervention’s educational workshops.

Conclusion

This research highlights the ways that physical, economic, and social side effects of PrEP can impact acceptability and persistence among trans women in Peru, amplifying and layering onto existing stressors including economic precarity. Understanding the unique experiences of trans women taking PrEP is crucial to informing tailored interventions to improve uptake and persistence.
The elements of end-of-life care provision in paediatric intensive care units: a systematic integrative review
Authors: Adistie Fanny , Neilson Susan , Shaw Karen L. , Bay Betul , Efstathiou Nikolaos
Published: July 2024

Background

Deaths in paediatric intensive care units (PICUs) are not uncommon. End-of-life care in PICUs is generally considered more challenging than other settings since it is framed within a context where care is focused on curative or life-sustaining treatments for children who are seriously ill. This review aimed to identify and synthesise literature related to the essential elements in the provision of end-of-life care in the PICU from the perspectives of both healthcare professionals (HCPs) and families.

Methods

A systematic integrative review was conducted by searching EMBASE, CINAHL, MEDLINE, Nursing and Allied Health Database, PsycINFO, Scopus, Web of Science, and Google Scholar databases. Grey literature was searched via Electronic Theses Online Service (EthOS), OpenGrey, Grey literature report. Additionally, hand searches were performed by checking the reference lists of all included papers. Inclusion and exclusion criteria were used to screen retrieved papers by two reviewers independently. The findings were analysed using a constant comparative method.

Results

Twenty-one studies met the inclusion criteria. Three elements in end-of-life care provision for children in the PICUs were identified: 1) Assessment of entering the end-of-life stage; 2) Discussion with parents and decision making; 3) End of life care processes, including care provided during the dying phase, care provided at the time of death, and care provided after death.

Conclusion

The focus of end-of-life care in PICUs varies depending on HCPs’ and families’ preferences, at different stages such as during the dying phase, at the time of death, and after the child died. Tailoring end-of-life care to families’ beliefs and rituals was acknowledged as important by PICU HCPs. This review also emphasises the importance of HCPs collaborating to provide the optimum end-of-life care in the PICU and involving a palliative care team in end-of-life care.
Menstrual hygiene management practice and associated factors among high school and preparatory school adolescent students in Debre Markos town, Northwest, Ethiopia: a mixed-method study
Authors: Worku Yichalem , Kassa Getachew Mullu , Mekonen Bekele , Desta Melaku , Bishaw Keralem Anteneh , Gedfaw Mihretie , Tesfaw Eyerus , Degu Genet , Shiferaw Aster
Published: July 2024

Background

Despite being a normal occurrence, menstruation requires hygienic care and is associated with a number of myths and wrongdoings. Menstrual hygiene issues have been linked to major health issues, such as urinary tract and reproductive tract infections. Consequently, the purpose of this study was to evaluate the management of menstrual hygiene and related aspects among teenage students in Debre Markos town, North West, Ethiopia.

Methods

From March 15 to April 15, 2019, teenage pupils in Debre Markos town participated in a cross-sectional mixed study. An in-depth interview and a self-administered structured questionnaire were used to gather data. Quantitative information was imported into Epi Data and then exported to SPSS for examination. A 95% Confidence Interval of p-value ≤ 0.05 was used to declare significance. The method of thematic content analysis was used to examine the qualitative data.

Result

This study comprised 531 individuals in total, with a 96.2% response rate. Approximately 260 adolescent females (49%, 95% CI: 39.2, 59.2) had good management practices for menstrual hygiene. Girls whose mothers were private employees (AOR: 0.3, 95% CI: 0.09, 0.99), self-employed (AOR: 0.52, 95% CI: 0.28, 0.98) and housewives (AOR: 0.53, 95% CI: 0.29, 0.98), and parent-adolescent discussions about menstruation (AOR: 1.62, 95% CI: 1.40, 3.34) were significantly associated with good menstrual hygiene management. Adolescence aged 17 years or older (AOR: 2.13, 95% CI: 1.32, 3.44) were found to have good knowledge regarding menstrual hygiene (AOR: 1.59, 95% CI: 1.43, 2.94). The qualitative study found that ignorance, an unfavorable school climate, and cultural and economic factors were the main causes of teenagers’ poor menstrual hygiene.

Conclusion

Nearly half of adolescent girls had good menstrual hygiene practice. Menstrual hygiene practice was associated with adolescent age, knowledge of menstruation, maternal occupational and discussion with parents. Girls had difficulty to manage menstrual hygiene due to poor knowledge, unsafe school environment, and cultural barriers. Thus, school-based programs aimed at improving knowledge towards menstrual hygiene management are needed.
Antiplasmodial potential of isolated xanthones from Mesua ferrea Linn. roots: an in vitro and in silico molecular docking and pharmacokinetics study
Authors: Konyanee Atthaphon , Chaniad Prapaporn , Chukaew Arnon , Payaka Apirak , Septama Abdi Wira , Phuwajaroanpong Arisara , Plirat Walaiporn , Punsawad Chuchard
Published: July 2024

Background

Malaria is a major global health concern, particularly in tropical and subtropical countries. With growing resistance to first-line treatment with artemisinin, there is an urgent need to discover novel antimalarial drugs. Mesua ferrea Linn., a plant used in traditional medicine for various purposes, has previously been investigated by our research group for its cytotoxic properties. The objective of this study was to explore the compounds isolated from M. ferrea with regards to their potential antiplasmodial activity, their interaction with Plasmodium falciparum lactate dehydrogenase (PfLDH), a crucial enzyme for parasite survival, and their pharmacokinetic and toxicity profiles.

Methods

The isolated compounds were assessed for in vitro antiplasmodial activity against a multidrug-resistant strain of P. falciparum K1 using a parasite lactate dehydrogenase (pLDH) assay. In vitro cytotoxicity against Vero cells was determined using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The interactions between the isolated compounds and the target enzyme PfLDH were investigated using molecular docking. Additionally, pharmacokinetic and toxicity properties were estimated using online web tools SwissADME and ProTox-II, respectively.

Results

Among the seven compounds isolated from M. ferrea roots, rheediachromenoxanthone (5), which belongs to the pyranoxanthone class, demonstrated good in vitro antiplasmodial activity, with the IC50 being 19.93 µM. Additionally, there was no toxicity towards Vero cells (CC50 = 112.34 µM) and a selectivity index (SI) of 5.64. Molecular docking analysis revealed that compound (5) exhibited a strong binding affinity of − 8.6 kcal/mol towards PfLDH and was stabilized by forming hydrogen bonds with key amino acid residues, including ASP53, TYR85, and GLU122. Pharmacokinetic predictions indicated that compound (5) possessed favorable drug-like properties and desired pharmacokinetic characteristics. These include high absorption in the gastrointestinal tract, classification as a non-substrate of permeability glycoprotein (P-gp), non-inhibition of CYP2C19, ease of synthesis, a high predicted LD50 value of 4,000 mg/kg, and importantly, non-hepatotoxic, non-carcinogenic, and non-cytotoxic effects.

Conclusions

This study demonstrated that compounds isolated from M. ferrea exhibit activity against P. falciparum. Rheediachromenoxanthone has significant potential as a scaffold for the development of potent antimalarial drugs.
The sensitivity outcome index system for home care of elderly liver transplant patients was developed based on the Omaha problem classification system
Authors: Wang Bin , Huang Xia , Liu Guofang , Zheng Taohua , Lin Hui , Qiao Yue , Sun Wenjuan
Published: July 2024

Objective

Based on the Omaha problem classification system, a sensitivity outcome index system for home nursing of elderly liver transplant patients was established.

Methods

Through a comprehensive literature review and rigorous application of the Delphi method, a panel of 20 experts completed two rounds of effective letter consultation to obtain expert consensus opinions. The contents of indicators were determined based on this process, and the analytic hierarchy process was employed to confirm the weightage assigned to each indicator. Consequently, we established a sensitivity outcome index system for home care in elderly liver transplant patients.

Results

The effective recovery rate of the questionnaire in two rounds of expert consultation was 100%, and the proportion of experts who gave opinions was 55% and 15%, respectively, indicating that the experts were highly active. The expert authority coefficients were calculated as 0.904 and 0.905, respectively, indicating a high degree of expert authority. In the second round, Kendall’s coordination coefficients for primary, secondary, and tertiary indicators were determined to be 0.419, 0.418, and 0.394 (P < 0.001), indicating that expert opinions tended to be consistent. Finally, we established a comprehensive sensitivity outcome index system comprising 4 first-level indexes, 20 s-level indexes, and 72 third-level indexes specifically designed for elderly liver transplantation patients.

Conclusion

The sensitivity outcome index system of home nursing for elderly liver transplant patients can provide theoretical basis for nursing staff to build accurate individualized continuous nursing model.
Residual effect of sequential 4-channel neuromuscular electrical stimulation evaluated by high-resolution manometry
Authors: Lim Jiwoon , Hyun Sung Eun , Kim Hayoung , Ryu Ju Seok
Published: July 2024

Background

High-resolution manometry (HRM) can quantify swallowing pathophysiology to evaluate the status of the pharynx. Sequential 4-channel neuromuscular electrical stimulation (NMES) was recently developed based on the normal contractile sequences of swallowing-related muscles. This study aimed to examine the effects of sequential 4-channel NMES for compensatory application during swallowing and to observe the residual effects after the application of NMES using HRM.

Results

Sequential 4-channel NMES significantly improved the HRM parameters, with respect to the maximal pressure and area of the velopharynx (VP), maximal pressure and area of the mesopharynx (MP), and upper esophageal sphincter (UES) activation and nadir duration. Furthermore, the improvement in the pressure and area variables of the VP and MP showed a tendency to maintain even when measured after NMES, but there are no significant differences.

Conclusions

The present study suggests that the sequential 4-channel NMES application of the suprahyoid and infrahyoid muscles during swallowing improves the pressure, area, and time variables of the oropharynx, as measured by HRM, and it is likely that the effects may persist even after stimulation.

Trial Registration Clinicaltrials.gov, registration number: NCT02718963 (initial release: 03/20/2016, actual study completion date: 06/24/2016, last release: 10/20/2020).
Global profiling of protein lactylation in microglia in experimental high-altitude cerebral edema
Authors: Jiang Xiufang , Gao Jiayue , Fei Xuechao , Geng Yanan , Yue Xiangpei , Shi Zibi , Cheng Xiang , Zhao Tong , Fan Ming , Wu Haitao , Zhao Ming , Zhu Lingling
Published: July 2024

Background

High-altitude cerebral edema (HACE) is considered an end-stage acute mountain sickness (AMS) that typically occurs in people after rapid ascent to 2500 m or more. While hypoxia is a fundamental feature of the pathophysiological mechanism of HACE, emerging evidence suggests that inflammation serves as a key risk factor in the occurrence and development of this disease. However, little is known about the molecular mechanism underlying their crosstalk.

Methods

A mouse HACE model was established by combination treatment with hypobaric hypoxia exposure and lipopolysaccharides (LPS) stimulation. Lactylated-proteomic analysis of microglia was performed to reveal the global profile of protein lactylation. Molecular modeling was applied to evaluate the 3-D modeling structures. A combination of experimental approaches, including western blotting, quantitative real-time reverse transcriptionpolymerase chain reaction (qRT-PCR), and enzyme-linked immunosorbent assay (ELISA), confocal microscopy and RNA interference, were used to explore the underlying molecular mechanisms.

Results

We found that hypoxia exposure increased the lactate concentration and lactylation in mouse HACE model. Moreover, hypoxia aggravated the microglial neuroinflammatory response in a lactate-dependent manner. Global profiling of protein lactylation has shown that a large quantity of lysine-lactylated proteins are induced by hypoxia and preferentially occur in protein complexes, such as the NuRD complex, ribosome biogenesis complex, spliceosome complex, and DNA replication complex. The molecular modeling data indicated that lactylation could affect the 3-D theoretical structure and increase the solvent accessible surface area of HDAC1, MTA1 and Gatad2b, the core members of the NuRD complex. Further analysis by knockdown or selectively inhibition indicated that the NuRD complex is involved in hypoxia-mediated aggravation of inflammation.

Conclusions

These results revealed a comprehensive profile of protein lactylation in microglia and suggested that protein lysine lactylation plays an important role in the regulation of protein function and subsequently contributes to the neuroinflammatory response under hypoxic conditions.