bluuebunny/crossref_metadata_2025 huggingface dataset:
Design of fuzzy sliding mode controller for islanded AC/DC hybrid microgrid with cyber‐attacks
Authors: Jiang He , Zhao Yan , Su Hanguang , Wang Yuzhong
Published: November 2023

This paper presents a fuzzy sliding mode control method for voltage control of islanded AC/DC hybrid microgrid. The T‐S fuzzy model is utilized to approximate initial non‐linear dynamic model of the microgrid. The fuzzy rules and membership functions are designed according to the coupling relationship between AC part and DC part. To reduce the influence of external disturbance and cyber‐attacks, an integral sliding mode controller is considered. Furthermore, a sufficient condition is proposed with attenuation performance to ensure the asymptotic stability of sliding motion. The set of gain matrices can be acquired via solving the linear matrix inequalities deduced from the Lyapunov stability analysis. The reachability to the proposed sliding mode surface can be guaranteed via switching control law based on a Lyapunov function. In addition, to eliminate chattering performance of the sliding mode control theory, the fuzzy logic controller is designed to optimize the switching region in boundary layers of saturation functions. Finally, the simulation results for the islanded AC/DC hybrid microgrid are implemented to verify the robustness and effectiveness of the designed fuzzy sliding mode control method.
Radar emitter structure identification based on stacked frequency sparse auto‐encoder network
Authors: Liu Lutao , Zhang Wei , Song Yu , Jiang Yilin , Yu Xiangzhen
Published: November 2023

In the current complex situations of electronic intelligence (ELINT), the authors present a radar emitter structure (RES) identification method based on deep learning at a new level to address the issue of incomplete cognitive information. Firstly, due to the fact that existing simulation data cannot fully reflect the structure features of the entire radar emitter, the structure feature‐level RES model is built using direct digital synthesiser (DDS) technology and radio frequency (RF) simulation platform. Afterwards, considering that the structure features are reflected in the frequency domain, a stacked frequency sparse auto encoder (sFSAE) network is constructed by adding a constraint term in frequency domain to the loss function of sparse auto encoder (SAE). Using deep learning to extract structure features with constraints in different domains is instructive for feature extraction techniques under variable operating parameters. Finally, the extracted structure features are input into the Softmax classifier to perform the identification from the radar signal to the RES. The experimental results show that the proposed method has high generalisation ability and robustness under different modulation types, different operating parameters and different signal to noise ratio (SNR). It also has a high identification rate even for untrained modulated signals.
COVID-stasis of Pregnancy: An Increased Prevalence of COVID-19 in Patients with Cholestasis
Authors: Holden Marissa G. , Schmiech Kathryn V. , Martinez Telleria Mariana , Sun Xinxin , Bender Whitney R. , Karjane Nicole W.
Published: November 2023

Objective To evaluate the relationship between intrahepatic cholestasis of pregnancy (ICP) and coronavirus disease 2019 (COVID-19).

Study Design We performed a retrospective cohort study of pregnant women undergoing induction of labor (IOL) at a single institution between May 2020 to January 2021. Primary exposure was diagnosis of intrahepatic cholestasis of pregnancy (ICP). The primary outcome was the prevalence of COVID-19 as determined by reverse-transcriptase polymerase chain reaction testing on nasopharyngeal swabs for SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) on routine admission testing. Secondary outcomes were abnormal laboratory values and adverse fetal outcomes. Logistic regression with log link analysis was performed comparing patients undergoing IOL for ICP compared with IOL for all other indications. The moderating effect of ethnicity was assessed by the interaction between ethnicity and ICP in a logistic regression model. The Wilcoxon rank-sum test and Fisher's exact test were performed for the secondary outcome analyses.

Results Over the course of the study, 596 patients underwent IOL: 24 for ICP and 572 for other indications. The overall prevalence of COVID-19 positivity in the cohort was 5.5% (33 of 596). Those with ICP were more likely to test positive for COVID-19 compared with those with other IOL indications (29.2 vs. 4.5%, RR = 6.4, 95% CI: 2.8–12.5, p < 0.001). All patients with ICP who tested positive for COVID-19 were Hispanic. To analyze the moderating effect of ethnicity, the results of the logistic model found the interaction between ethnicity and ICP to not be significant (p = 0.991). In patients with ICP, the median AST (aspartate aminotransferase) was higher than those with COVID-19 (p = 0.0182). There were no adverse fetal outcomes in the ICP group.

Conclusion In this single-site retrospective cohort study, we demonstrated an increased prevalence of COVID-19 in those with ICP in general and among Hispanic patients specifically. Despite this difference, there was no increased risk of adverse fetal outcomes.

Key Points
Preoperative Application of Chlorhexidine to Reduce Infection with Cesarean Delivery after Labor (PRACTICAL): A Randomized Clinical Trial
Authors: DeBolt Chelsea A. , Rao Manasa G. , Warren Leslie , Johnson Shaelyn , Rekawek Patricia , Kaplowitz Elianna , Overbey Jessica , Paul Keisha , Tavella Nicola , Monro Johanna , Stone Joanne , Bianco Angela
Published: November 2023

Objective To evaluate whether use of both preoperative 2% chlorhexidine gluconate abdominal cloth and 4% chlorhexidine gluconate vaginal scrub is effective in reducing surgical site infections (SSIs) in patients undergoing cesarean delivery after labor.

Study Design This is a single-center, randomized clinical trial in which patients were randomized 1:1 to receive 2% chlorhexidine gluconate cloth applied to the abdomen in addition to the application of 4% chlorhexidine gluconate vaginal scrub versus standard of care. The primary outcome was rate of SSIs, including endometritis, by 6 weeks postdelivery. The secondary outcomes were other wound complications (erythema at the operative site, skin separation, drainage, fever, hematoma, seroma) by 6 weeks postdelivery, hospital readmission for wound complications, and day of discharge after cesarean delivery.

Results A total of 319 patients between September 2018 and February 2021 met eligibility criteria: 160 were randomized to the chlorhexidine gluconate abdominal cloth and vaginal scrub group and 159 were randomized to the standard of care group. The groups did not have significant differences in maternal demographic characteristics. Of the 302 (95%) individuals included in primary analysis, there was no statistically significant difference in SSI and endometritis rate by 6 weeks postdelivery (6.6% in chlorhexidine vs. 5.3% standard of care, p = 0.65). Secondary outcomes were also similar among the two groups.

Conclusion The combination of preoperative 2% chlorhexidine gluconate abdominal cloth and 4% chlorhexidine gluconate vaginal scrub does not appear to reduce the risk of SSI with cesarean delivery after trial of labor when compared with standard of care.

Key Points
The Molecular Structure of Fibrinogen
Authors: Murano Genesio
Published: November 2023

Seminars in Thrombosis and Hemostasis (STH) celebrates 50 years of publishing in 2024. To celebrate this landmark event, STH is republishing some archival material. This manuscript represents the first full paper ever published in STH. The manuscript published without an abstract, and essentially covered in considerable detail the molecular structure of fibrinogen, as was known at that time. Fittingly, it covers some historical perspectives, the physicochemical properties and structure of fibrinogen across several species of animals (including humans) and its transformation into fibrin. We hope the readers of STH enjoy this journey into the past. This manuscript is accompanied by a Commentary that reflects on this past, as well as the journey towards contemporary understanding of the molecular structure of fibrinogen. As this is a republication of archival material, transformed into a modern format, we apologise in advance for any errors introduced during this transformation.
Primary Free Flaps for Coverage and Reconstruction in Acute Facial Trauma
Authors: Ravula Parvati , R. Srikanth , Khan Pathan Subhan , Nuvvula Rambabu , Yellinedi Rajesh
Published: November 2023

Background Acute facial trauma in motor vehicle accident defects may be associated with skeletal, neuromuscular, or mucosal losses. Simultaneous repair of the critical structures in these defects mandates the use of flap cover; paucity of local tissues necessitates the use of free skin flaps.

Materials and Methods Six free flap reconstructions for acute facial trauma defects over a 10-year period were reviewed. The defect location, associated injuries, flap choice, additional reconstructive procedures, and flap outcomes were analyzed.

Results There were four males and two females with ages between 18 and 63 years. Four defects were located in the lateral face and scalp, and two in the lower central face. Defect size ranged between 96 and 346 cm2. There were fractures in three, facial nerve injuries in two, and loss of facial muscles in one. Five free flaps were anterolateral thigh flap; simple and composite, one was a radial artery forearm flap. Recipient pedicles were the superficial temporal vessels in two and facial vessels in four cases. There were no re-explorations but one flap necrosed on 7th postoperative day on account of invasive aspergillosis.

Discussion Use of free flaps for ballistic wounds is common. In uncommon nonballistic traumatic facial defects, the location, nature of the defect, and type of associated injuries and need for simultaneous reconstructions may dictate the use of free flaps and permit a one stage debridement, flap coverage, and a simultaneous reconstruction of lost functional units.

Conclusion Free flap coverage in high velocity acute facial trauma defects offers a better possibility for primary reconstruction of associated facial injuries and helps in achieving better functional outcomes.
‘The first, but not the last’: women’s descriptive and substantive representation in the 2021 Scottish Parliament election
Authors: Belknap Emilia , Kenny Meryl
Published: November 2023

The 2021 Scottish Parliament election delivered the most diverse range of MSPs in Holyrood’s history, including a record number of women. We situate the 2021 election within its wider context, evaluating dynamics of party feminisation over time. Drawing on original candidate data and analysis of party manifestos across the main Scottish parties, we assess parties’ substantive appeals to women voters; the integration of different groups of women as candidates, MSPs and leaders; and the adoption and implementation of gender quotas. We find evidence of descriptive and substantive party feminisation in Scotland over time—including increases in both the amount and diversity of party manifesto pledges directed at women voters; improvements in the number and diversity of women selected and elected; and the increasing diffusion of, and inter-party competition over, gender quota measures. Yet we also find that progress has been uneven across parties and that some groups of women continue to be descriptively and substantively under-represented in Scottish politics.
Friction or cooperation? Boosting the global economy and fighting climate change in the post-pandemic era
Authors: Yang Zhenshan , Wei Jianan , Ge Quansheng
Published: November 2023

In the post-COVID-19 pandemic era, the world faces a choice between trade protection and cooperation. However, current literature provides very little information on the benefit or loss of trade friction or cooperation on global economy and climate mitigation. This study applied the Global Trade Analysis Project model to assess the impacts of trade friction on global economic recovery and climate change. The results indicated that international trade friction can both delay global economic recovery and affect CO2 emission reduction. The shocks of consumption reduction and production suspension have a higher marginal effect in developing and emerging economies, whereas trade friction has a higher effect on developed countries. Trade friction has more negative economic effects for developed countries, but developing countries cannot reduce CO2 emissions proportionally with the decrease in trade and related production. In the post-pandemic era, if the global trade barrier increases, the world may face a co-occurring economic decline and an increase in or low abatement of carbon emissions.
How do online users respond to crowdsourced fact-checking?
Authors: Panizza Folco , Ronzani Piero , Morisseau Tiffany , Mattavelli Simone , Martini Carlo
Published: November 2023

Recently, crowdsourcing has been proposed as a tool for fighting misinformation online. Will internet users listen to crowdsourced fact-checking, and how? In this experiment we test how participants follow others’ opinions to evaluate the validity of a science-themed Facebook post and examine which factors mediate the use of this information. Participants observed a post presenting either scientific information or misinformation, along with a graphical summary of previous participants’ judgements. Even though most participants reported not having used information from previous raters, their responses were influenced by previous assessments. This happened regardless of whether prior judgements were accurate or misleading. Presenting crowdsourced fact-checking however did not translate into the blind copying of the majority response. Rather, participants tended to use this social information as a cue to guide their response, while also relying on individual evaluation and research for extra information. These results highlight the role of individual reasoning when evaluating online information, while pointing to the potential benefit of crowd-sourcing-based solutions in making online users more resilient to misinformation.
Variations in functional traits and resilience of Inga vera subsp. affinis under flooding and drought conditions
Authors: Müller Padilha Feitosa Rosana , Braghin Louizi de Souza Magalhães , Pastorini Lindamir Hernandez , Romagnolo Mariza Barion
Published: November 2023

Context The dynamics of a plant population are influenced by both biotic and abiotic factors that interfere with the phenotype of the individual, indicating how species develop in the occupied environment. Riparian plant species are subject to environmental fluctuations, such as periodic flooding regimes and severe droughts, which may result from climate change. Aims The aim of this study was to evaluate the morphological growth responses of individuals of Inga vera subsp. affinis (DC.) T.D.Penn. in controlled drought and flooding conditions by observing variations in functional traits using controlled experiments. Methods Individuals were subjected to partial flooding and dry periods, and functional traits, such as leaf area, root height and dry mass, were analysed. The difference between treatments was tested using unifactorial statistical analysis of variance (ANOVA). Key results Fifteen-day-old individuals of Inga vera subsp. affinis kept under flooding conditions showed lower root height and dry mass compared to plants exposed to drought treatment; 50-day-old plants under flooding conditions also presented lower root dry mass than did plants in drought treatment, maintaining the result after the resilience period. Conclusions Root dry mass was the most affected variable in individuals of Inga vera subsp. affinis under flooding conditions, even after the recovery period. In general, plants in the control and drought treatments did not differ significantly considering the analysed variables. Implications Given the current climate change conditions, an increase in the frequency and duration of extreme events, such as floods and droughts, is expected. Thus, understanding how phenotypical traits of species already adapted to flooding conditions behave under water stress and waterlogged conditions for longer periods of time is essential to explain plant responses and predict future survival scenarios for the species.
Impact of water stress during reproductive development on seed dormancy in Bromus diandrus Roth and Lolium rigidum Gaudin
Authors: Ramiz Zarka , Malone Jenna , Preston Christopher , Gill Gurjeet
Published: November 2023

Context Bromus diandrus Roth and Lolium rigidum Gaudin are important weeds of cereal-based cropping systems of Australian agriculture. Adaptation to environmental stresses, protracted seed germination and herbicide resistance have made these weeds serious threats to crop production. Aims Studies were undertaken to determine the impact of moisture stress during reproductive development on the extent of seed dormancy and the expression of genes involved with gibberellic acid and abscisic acid synthesis. Methodology A pot study was undertaken at two locations, with two populations each of B. diandrus and L. rigidum. Water stress was applied from either the GS31 or GS60 stage until seed maturation, along with a well-watered treatment. Seeds of stressed vs well-watered treatments were assessed for seed dormancy and the expression of ABA1 and GA20ox genes using quantitative polymerase chain reaction. Results The seeds from GS31 treatment, where plants experienced the longest water stress, were most dormant in both weed species. Water stress treatments altered the expression of the GA20ox gene, which was correlated with the level of dormancy in seeds of B. diandrus and L. rigidum. Conclusions This investigation has provided clear evidence of the impact of water stress on seed dormancy and on expression of genes involved in regulating seed dormancy in these weed species. Implications As spring rainfall in the Australian agricultural landscape is highly variable, weeds are likely to experience variable levels of water stress during reproductive development, which in turn is likely to influence seed dormancy, weed seedling emergence and effectiveness of pre-sowing weed management next season.
Learning by Doing: Educational Tourism at Mount Danxiashan
Authors: Li Guiqing , Xu Ding
Published: November 2023

Educational tourism is a fast-growing market in China. Since Mount Danxiashan was listed as a Global Geopark and World Heritage Site in 2010, educational tourism has rapidly grown in the local community, becoming a flagship tourism product. This makes Mount Danxiashan an informative case for understanding educational tourism development and community participation. The present case study has two foci. First, we articulate the stakeholder collaboration mode at Mount Danxiashan. Various stakeholders, including but not limited to the administrative authority, research and education institutes, and local communities, co-determined to develop educational tourism as the best practice for local resources. The collaboration mode and the educational focus are critical in sustaining local development. Second, we depict the learning trajectory of the local community. Led by local intellectual elites, residents acquired both tourism- and nature-related knowledge and participated in educational tourism. This enabled them to design and deliver nature and heritage education curricula for tourists. Moreover, this process encouraged and empowered villagers to co-create new knowledge.

Information

© The Authors 2023
Diagnostic impact of RNA-based next-generation sequencing fusion panel for solid tumors: A single-institution experience
Authors: Fei Fei , Kunder Christian A , Ho Chandler , Zehnder James L , Tomasello Gianna , Fung Eula , Suarez Carlos J
Published: November 2023

Objectives

Gene rearrangements frequently act as oncogenic driver mutations and determine the onset and progression of cancer. RNA-based next-generation sequencing (NGS) is being used with increasing frequency for solid tumors. The purpose of our study is to investigate the feasibility and utility of an RNA-based NGS fusion panel for solid tumors.

Methods

We conducted a retrospective, single-institution review of fusion panels requested between May 2022 and March 2023. Demographic, clinical, pathologic, and molecular findings of the patients were reviewed. The utility of the RNA-based NGS fusion panel for the pathologic diagnosis of solid tumors was assessed.

Results

Our study included 345 cases, and a fusion event was identified in 24.3% (78/321) of cases. Among the 110 cases submitted for diagnostic purposes, a fusion event was detected in 42.7% (47/110) of cases. The results led to refinement or clarification of the initial diagnosis in 31.9% (15/47) of cases and agreement or support for the initial diagnosis in 59.6% (28/47) of cases. Furthermore, our study indicated that the overall cellularity (tumor and normal tissue) of the tested specimen influences the success of the testing process.

Conclusions

In summary, this study demonstrated the feasibility and utility of an RNA-based NGS fusion panel for a wide variety of solid tumors in the appropriate clinicopathologic context. These findings warrant further validation in larger studies involving multiple institutional patient cohorts.
A mixed-methods study on the use of a nongynecological virtual microscopy platform in cytology education
Authors: Chiou Paul Z , Jia Yuane
Published: November 2023

Objectives

Expanding the virtual microscopy (VM) slide collection for nongynecological cytology is important to teaching. In a mixed-methods approach, this study evaluated VM’s performance and user experience to determine its feasibility and usage in an educational setting.

Methods

From September through October 2022, the students reviewed 44 whole-slide imaged nongynecological slides. The concordance rate with reference diagnosis was compared with that from light microscopy (LM) from 4 months earlier. In addition to assessing the overall performance from VM, imaged urinary cytology’s accuracy was reviewed for both urinary and nonurinary cytology. Finally, the students’ weekly feedback logs were analyzed to gain insights for improving the digital screening experience.

Results

The overall nongynecological diagnostic accuracy was significant between the 2 screening platforms (P < .001), favoring LM over the VM platform. Light microscopy also performed better than VM in urine cytology cases, with 84.2% concordance against reference diagnosis, compared with 61.1% for the VM platform (P = .03). As for the accuracy of nonurinary cases, its glass slide (LM) agreement with the reference diagnosis was also superior at 84.8%, compared with 58.8% for VM (P = .03). Finally, the overarching theme discerned from reviewing the user logs was concern over image quality, which was mentioned 76 times.

Conclusions

The VM results were poorer compared with LM in our validation. Its use seems promising, but more focus is needed to improve the VM screening platform.
scTopoGAN: unsupervised manifold alignment of single-cell data
Authors: Singh Akash , Biharie Kirti , Reinders Marcel J T , Mahfouz Ahmed , Abdelaal Tamim
Published: November 2023

Motivation

Single-cell technologies allow deep characterization of different molecular aspects of cells. Integrating these modalities provides a comprehensive view of cellular identity. Current integration methods rely on overlapping features or cells to link datasets measuring different modalities, limiting their application to experiments where different molecular layers are profiled in different subsets of cells.

Results

We present scTopoGAN, a method for unsupervised manifold alignment of single-cell datasets with non-overlapping cells or features. We use topological autoencoders (topoAE) to obtain latent representations of each modality separately. A topology-guided Generative Adversarial Network then aligns these latent representations into a common space. We show that scTopoGAN outperforms state-of-the-art manifold alignment methods in complete unsupervised settings. Interestingly, the topoAE for individual modalities also showed better performance in preserving the original structure of the data in the low-dimensional representations when compared to other manifold projection methods. Taken together, we show that the concept of topology preservation might be a powerful tool to align multiple single modality datasets, unleashing the potential of multi-omic interpretations of cells.

Availability and implementation

Implementation available on GitHub (https://github.com/AkashCiel/scTopoGAN). All datasets used in this study are publicly available.
A Bayesian approach to differential edges with probabilistic interactions: applications in association and classification
Authors: Huang Yu-Jyun , Lai Ying-Ju , Hsiao Chuhsing Kate
Published: November 2023

Motivation

Differential network (D-Net) analysis has attracted great attention in systems biology for its ability to identify genetic variations in response to different conditions. Current approaches either estimate the condition-specific networks separately followed by post-procedures to determine the differential edges or estimate the D-Net directly. Both types of analysis overlook the probabilistic inference and can only provide deterministic inference of the edges.

Results

Here, we propose a Bayesian solution and translate the probabilistic estimation in the regression model to an inferential D-Net analysis for genetic association and classification studies. The proposed PRobabilistic Interaction for Differential Edges (PRIDE) focuses on inferring the D-Net with uncertainty so that the existence of the differential edges can be evaluated with probability and even prioritized if comparison among these edges is of interest. The performance of the proposed model is compared with state-of-the-art methods in simulations and is demonstrated in glioblastoma and breast cancer studies. The proposed PRIDE performs comparably to or outperforms most existing tools under deterministic evaluation criteria. Additionally, it offers the unique advantages, including prioritizing the differential edges with probabilities, highlighting the relative importance of hub nodes, and identifying potential sub-networks in a D-Net.

Availability and implementation

All the data analyzed in this research can be downloaded at https://xenabrowser.net/datapages/. The R code for implementing PRIDE is available at https://github.com/YJGene0806/PRIDE_Code.
Term-BLAST-like alignment tool for concept recognition in noisy clinical texts
Authors: Groza Tudor , Wu Honghan , Dinger Marcel E , Danis Daniel , Hilton Coleman , Bagley Anita , Davids Jon R , Luo Ling , Lu Zhiyong , Robinson Peter N
Published: November 2023

Motivation

Methods for concept recognition (CR) in clinical texts have largely been tested on abstracts or articles from the medical literature. However, texts from electronic health records (EHRs) frequently contain spelling errors, abbreviations, and other nonstandard ways of representing clinical concepts.

Results

Here, we present a method inspired by the BLAST algorithm for biosequence alignment that screens texts for potential matches on the basis of matching k-mer counts and scores candidates based on conformance to typical patterns of spelling errors derived from 2.9 million clinical notes. Our method, the Term-BLAST-like alignment tool (TBLAT) leverages a gold standard corpus for typographical errors to implement a sequence alignment-inspired method for efficient entity linkage. We present a comprehensive experimental comparison of TBLAT with five widely used tools. Experimental results show an increase of 10% in recall on scientific publications and 20% increase in recall on EHR records (when compared against the next best method), hence supporting a significant enhancement of the entity linking task. The method can be used stand-alone or as a complement to existing approaches.

Availability and implementation

Fenominal is a Java library that implements TBLAT for named CR of Human Phenotype Ontology terms and is available at https://github.com/monarch-initiative/fenominal under the GNU General Public License v3.0.
Parallel phenotypic plasticity and divergent ecological strategies in morphologically and molecularly similar sympatric sponge species
Authors: Wulff Janie
Published: November 2023

How can phenotypic plasticity promote or impede adaptive change and diversification? Answering this key question can be experimentally intractable, but closely related clonal species allow a direct approach: experimentally provoking phenotypic plasticity to assess the adaptive significance of both plastic traits and species differences. Two common Caribbean sponge species, Aplysina fulva and A. cauliformis, are nearly indistinguishable molecularly, and share growth form, habitat, and geographical range. This raises questions about species boundaries, within-species variability, and mechanisms of speciation. To distinguish phenotypic plasticity from genetic variation, and learn how plasticity might influence adaptive—and divergent—evolution, I: (i) quantified morphological and ecological characters, (ii) revealed phenotypic plasticity by growing clonemates in different environments, and (iii) related plastic morphological features to ecological function. Characters included skeletal fibre density, biomechanical properties, vulnerability to parasites and predators, wound healing, transport pathways, propagation by fragments, population dynamics, and growth and survival in settings differing in food, sunlight, predators, and water motion. Transplanting both species to a different environment elicited parallel plasticity in the same traits. Combined comparative and experimental data reveal integrated suites of ecologically relevant characters that clearly distinguish these species and allow interpretation of adaptive significance of plastic characters that may underlie divergence.
EHD1 impaired decidualization of endometrial stromal cells in recurrent implantation failure: role of SENP1 in modulating progesterone receptor signalling
Authors: Zhang Hui , Kong Liping , Cao Zhiwen , Zhu Yinchun , Jiang Yue , Wang Xiaoying , Jiang Ruiwei , Liu Yang , Zhou Jidong , Kang Yu , Zhen Xin , Kong Na , Wu Min , Yan Guijun , Sun Haixiang
Published: November 2023

Recurrent implantation failure (RIF) patients exhibit poor endometrial receptivity and abnormal decidualization with reduced effectiveness and exposure to progesterone, which is an intractable clinical problem. However, the associated molecular mechanisms remain elusive. We found that EH domain containing 1 (EHD1) expression was abnormally elevated in RIF and linked to aberrant endometrial decidualization. Here we show that EHD1 overexpressed in human endometrial stromal cells significantly inhibited progesterone receptor (PGR) transcriptional activity and the responsiveness to progesterone. No significant changes were observed in PGR mRNA levels, while a significant decrease in progesterone receptor B (PRB) protein level. Indeed, EHD1 binds to the PRB protein, with the K388 site crucial for this interaction. Overexpression of EHD1 promotes the SUMOylation and ubiquitination of PRB, leading to the degradation of the PRB protein. Supplementation with the de-SUMOylated protease SENP1 ameliorated EHD1–repressed PRB transcriptional activity. To establish a functional link between EHD1 and the PGR signalling pathway, sg-EHD1 were utilized to suppress EHD1 expression in HESCs from RIF patients. A significant increase in the expression of prolactin and insulin-like growth factor-binding protein 1 was detected by interfering with the EHD1. In conclusion, we demonstrated that abnormally high expression of EHD1 in endometrial stromal cells attenuated the activity of PRB associated with progesterone resistance in a subset of women with RIF.
Maximum likelihood estimation for semiparametric regression models with interval-censored multistate data
Authors: Gu Yu , Zeng Donglin , Heiss Gerardo , Lin D Y
Published: November 2023

Interval-censored multistate data arise in many studies of chronic diseases, where the health status of a subject can be characterized by a finite number of disease states and the transition between any two states is only known to occur over a broad time interval. We relate potentially time-dependent covariates to multistate processes through semiparametric proportional intensity models with random effects. We study nonparametric maximum likelihood estimation under general interval censoring and develop a stable expectation-maximization algorithm. We show that the resulting parameter estimators are consistent and that the finite-dimensional components are asymptotically normal with a covariance matrix that attains the semiparametric efficiency bound and can be consistently estimated through profile likelihood. In addition, we demonstrate through extensive simulation studies that the proposed numerical and inferential procedures perform well in realistic settings. Finally, we provide an application to a major epidemiologic cohort study.
Hate Crime and Class Vulnerability: A Case Study of White Nationalist Violence Against Unhoused Indigenous People
Authors: Urbanik Marta-Marika , Maier Katharina , Tetrault Justin E C , Greene Carolyn
Published: November 2023

Advocates and academics have increasingly called on governments to recognize anti-homeless violence as a hate crime and type of domestic extremism, representing a broader trend in Westernized countries for responding to social issues through anti-hate policies. Can these approaches protect unhoused people? Drawing upon ethnographic interviews and observation with 50 unhoused community members in a Canadian city, we outline their experiences with anti-homeless and anti-Indigenous violence. Our findings show how hate crime approaches often (1) fail to consider intersectionality, especially how class contributes to vulnerability, and (2) overlook place-based victimization and how institutions enable class vulnerability. We call for more localized analyses of hate crime and introduce the concept of ‘cumulative risk of hate crime victimization’ to help address intersectionality.
Guidelines for cold urticaria management established by the Centre of Evidence of Dermatology and the Urticaria Group of the French Society of Dermatology
Authors: Bréhon Alice , Bensefa-Colas Lynda , D’Andrea Cyril , Jeziorski Eric , Lehucher-Michel Marie-Pascale , Lequipe Johan , Marmion Nicolas , Mathelier-Fusade Pascale , Mercier Jean-Christophe , Sebatigita Georges , Tannous Julio , Vigan Martine , Guillot Bernard , Du-Thanh Aurélie , Soria Angèle
Published: November 2023

This research letter provides guidelines for cold urticaria management established by the Centre of Evidence of Dermatology and the Urticaria Group of the French Society of Dermatology.
Working Together to Create Meaning: Narratives of Care Leavers in Eastern Europe
Authors: Kessler Zoë , Levy Susan , Smith Mark
Published: November 2023

This article uses life history research to reveal a new understanding of institutional care. The study draws on interviews with care leavers from a Latvian orphanage who narrate life histories and identify critical life events and moments of resistance to times of adversity. The methodology intentionally positioned study nineteen care leavers as experts on their own experiences, focusing on three main life phases: before, during and after living in care. Findings deconstructed and brought nuance to stereotypes and dominant assumptions about people growing up in care, providing alternative narratives of self-reliance and resistance to adversity. Recommendations for practice and policy are discussed. This rich knowledge base can be used to support and inform the practice of children in the care system in Latvia and more widely.
Nigrostriatal tau pathology in parkinsonism and Parkinson’s disease
Authors: Chu Yaping , Hirst Warren D , Federoff Howard J , Harms Ashley S , Stoessl A Jon , Kordower Jeffrey H
Published: November 2023

While Parkinson’s disease remains clinically defined by cardinal motor symptoms resulting from nigrostriatal degeneration, it is now appreciated that the disease commonly consists of multiple pathologies, but it is unclear where these co-pathologies occur early in disease and whether they are responsible for the nigrostriatal degeneration.

For the past number of years, we have been studying a well-characterized cohort of subjects with motor impairment that we have termed mild motor deficits. Motor deficits were determined on a modified and validated Unified Parkinson’s Disease Rating Scale III but were insufficient in degree to diagnose Parkinson’s disease. However, in our past studies, cases in this cohort had a selection bias, as both a clinical syndrome in between no motor deficits and Parkinson’s disease, plus nigral Lewy pathology as defined post-mortem, were required for inclusion. Therefore, in the current study, we only based inclusion on the presence of a clinical phenotype with mild motor impairment insufficient to diagnose Parkinson’s disease. Then, we divided this group further based upon whether or not subjects had a synucleinopathy in the nigrostriatal system.

Here we demonstrate that loss of nigral dopaminergic neurons, loss of putamenal dopaminergic innervation and loss of the tyrosine hydroxylase-phenotype in the substantia nigra and putamen occur equally in mild motor deficit groups with and without nigral alpha-synuclein aggregates. Indeed, the common feature of these two groups is that both have similar degrees of AT8 positive phosphorylated tau, a pathology not seen in the nigrostriatal system of age-matched controls. These findings were confirmed with early (tau Ser208 phosphorylation) and late (tau Ser396/Ser404 phosphorylation) tau markers. This suggests that the initiation of nigrostriatal dopaminergic neurodegeneration occurs independently of alpha-synuclein aggregation and can be tau mediated.
Efficient transmission of human prion diseases to a glycan-free prion protein-expressing host
Authors: Cracco Laura , Cali Ignazio , Cohen Mark L , Aslam Rabail , Notari Silvio , Kong Qingzhong , Newell Kathy L , Ghetti Bernardino , Appleby Brian S , Gambetti Pierluigi
Published: November 2023

It is increasingly evident that the association of glycans with the prion protein (PrP), a major post-translational modification, significantly impacts the pathogenesis of prion diseases. A recent bioassay study has provided evidence that the presence of PrP glycans decreases spongiform degeneration and disease-related PrP (PrPD) deposition in a murine model. We challenged (PRNPN181Q/197Q) transgenic (Tg) mice expressing glycan-free human PrP (TgGlyc−), with isolates from sporadic Creutzfeldt–Jakob disease subtype MM2 (sCJDMM2), sporadic fatal insomnia and familial fatal insomnia, three human prion diseases that are distinct but share histotypic and PrPD features. TgGlyc− mice accurately replicated the basic histotypic features associated with the three diseases but the transmission was characterized by high attack rates, shortened incubation periods and a greatly increased severity of the histopathology, including the presence of up to 40 times higher quantities of PrPD that formed prominent deposits. Although the engineered protease-resistant PrPD shared at least some features of the secondary structure and the presence of the anchorless PrPD variant with the wild-type PrPD, it exhibited different density gradient profiles of the PrPD aggregates and a higher stability index. The severity of the histopathological features including PrP deposition appeared to be related to the incubation period duration. These findings are clearly consistent with the protective role of the PrP glycans but also emphasize the complexity of the conformational changes that impact PrPD following glycan knockout. Future studies will determine whether these features apply broadly to other human prion diseases or are PrPD-type dependent.
Delusional infestation treated with risperidone: a series of 27 patients
Authors: Guedes Nycolle L K O , Dwan Alexandre J , Gerlero Paula , Nico Marcello M S
Published: November 2023

Background

Patients with delusional infestation (DI) frequently refuse to be treated with psychoactive drugs. In the past, pimozide was commonly used as a first-line agent but is now prescribed more rarely. Risperidone was first used to treat DI in 1995. A recent review identified 12 studies that evaluated the use of risperidone in 43 patients with DI.

Objectives

To study the characteristics of and therapeutic results in patients with DI treated with risperidone at a university medical centre in São Paulo, Brazil.

Methods

We performed a retrospective study of patients with DI treated with risperidone at a dermatological university clinic since 2016. Records were reviewed for personal data and findings related to treatment.

Results

Twenty-seven patients were studied (20 women and 7 men). The maintenance dose of risperidone varied from 1 mg three times weekly to 8 mg daily. Control of symptoms was achieved in the majority of patients. A reduction in dosage due to side-effects was seen in four patients; risperidone had to be switched to another antipsychotic in three cases, despite a good response. Only one patient did not respond to risperidone.

Conclusions

Risperidone is an effective, well-tolerated and safe treatment for delusional parasitosis. Adequate follow-up is mandatory in order to obtain long-term control of symptoms.
Hyperhidrosis: a patient's perspective
Authors: O’Reilly Marese , Affleck Andrew
Published: November 2023

The patient explores the profound impact of hand and feet hyperhidrosis and its associated impact on his life both from a physical and psychological perspective. Primary hyperhidrosis (PH) is a common condition with variable estimated prevalence rates ranging from 4.8% in the USA to 14.5% in Shanghai, China. Bilateral symmetrical palmar hyperhidrosis is a common form of idiopathic PH.
Fasting durations of Steller sea lion pups vary among subpopulations—evidence from two plasma metabolites
Authors: Crawford Stephanie G , Coker Robert H , O’Hara Todd M , Breed Greg A , Gelatt Tom , Fadely Brian , Burkanov Vladimir , Rivera Patricia M , Rea Lorrie D
Published: November 2023

Geographic differences in population growth trends are well-documented in Steller sea lions (Eumetopias jubatus), a species of North Pacific pinniped listed under the U.S. Endangered Species Act in 1990 following a marked decline in population abundance that began during the 1970s. As population growth is intrinsically linked to pup production and survival, examining factors related to pup physiological condition provides useful information to management authorities regarding potential drivers of regional differences. During dam foraging trips, pups predictably transition among three fasting phases, distinguished by the changes in the predominant metabolic byproduct. We used standardized ranges of two plasma metabolites (blood urea nitrogen and β–hydroxybutyrate) to assign pups to fasting categories (n = 1528, 1990–2016, 12 subpopulations): Recently Fed–Phase I (digestion/assimilation–expected hepatic/muscle glycogen usage), Phase II (expected lipid utilization), transitioning between Phases II–III (expected lipid utilization with increased protein reliance), or Phase III (expected protein catabolism). As anticipated, the majority of pups were classified as Recently Fed–Phase I (overall mean proportion = 0.72) and few pups as Phase III (overall mean proportion = 0.04). By further comparing pups in Short (Recently Fed–Phase II) and Long (all other pups) duration fasts, we identified three subpopulations with significantly (P < 0.03) greater proportions of pups dependent upon endogenous sources of energy for extended periods, during a life stage of somatic growth and development: the 1) central (0.27 ± 0.09) and 2) western (0.36 ± 0.13) Aleutian Island (declining population trend) and 3) southern Southeast Alaska (0.32 ± 0.06; increasing population trend) subpopulations had greater Long fast proportions than the eastern Aleutian Islands (0.10 ± 0.05; stabilized population). Due to contrasting population growth trends among these highlighted subpopulations over the past 50+ years, both density-independent and density-dependent factors likely influence the dam foraging trip duration, contributing to longer fasting durations for pups at some rookeries.
Improved Clinical Outcomes With Early Anti-Tumour Necrosis Factor Alpha Therapy in Children With Newly Diagnosed Crohn’s Disease: Real-world Data from the International Prospective PIBD-SETQuality Inception Cohort Study
Authors: Klomberg Renz C W , van der Wal Hella C , Aardoom Martine A , Kemos Polychronis , Rizopoulos Dimitris , Ruemmele Frank M , Charrout Mohammed , Escher Hankje C , Croft Nicholas M , de Ridder Lissy ,   PIBD-SETQuality collaborative group, Milovanovich Ivan D , Ashton James J , Henderson Paul , Ledder Oren , de Meij Tim G J , Hansen Richard , Hummel Thalia Z , Arai Katsuhiro , Rodrigues Astor , Cameron Fiona , Koletzko Sibylle , Muhammed Rafeeq , Nedelkopoulou Natalia
Published: November 2023

Background and Aims

Treatment guidelines for paediatric Crohn’s disease [CD] suggest early use of anti-tumour necrosis factor alpha [anti-TNFα] in high-risk individuals. The aim is to evaluate the effect of early anti-TNF in a real-world cohort.

Methods

Children with newly diagnosed CD were prospectively recruited at 28 participating sites of the international observational PIBD-SETQuality study. Outcomes were compared at 3 months, 1 and 2 years between patients receiving early anti-TNF [<90 days after diagnosis] and those not receiving early anti-TNF. Outcomes included sustained steroid-free remission [SSFR] without treatment intensification [specified as SSFR*] and sustained steroid-free mild/inactive disease without treatment intensification [specified as SSFMI*]. Penalised logistic regression model-based standardisation was applied to estimate the relative risks [RR] of early therapy on outcomes. RRs were estimated for high-risk and low-risk patients, based on presence of predictors of poor outcome [POPOs] and disease activity at diagnosis.

Results

In total, 331 children (median age 13.9 years [IQR 12.2–15.3]) were enrolled, with 135 [41%] receiving early anti-TNF. At 1 year, patients on early anti-TNF had higher rates of SSFR* [30% vs 14%, p <0.001] and SSFMI* [69% vs 33%, p <0.001], with RRs of 2.95 [95% CI 1.63-5.36] and 4.67 [95% CI 2.46-8.87], respectively. At 1 year, the RRs for SSFMI* were higher, and statistically significant in high-risk patients, i.e. those with moderate/severe disease compared with mild/inactive disease at diagnosis (5.50 [95% CI 2.51-12.05] vs 2.91 [95% CI 0.92-9.11]), and those with any POPO compared with no POPO (5.05 [95% CI 2.45-10.43] vs 3.41 [95% CI 0.54-21.7]).

Conclusion

In this cohort of children with newly-diagnosed CD, early anti-TNF demonstrated superior effectiveness in high-risk patients.