bluuebunny/crossref_metadata_2025 huggingface dataset:
Surgery or Non-surgical Treatment of ≤8 mm Non-small Cell Lung Cancer: A Population-Based Study
Authors: Shen Jianfei , Zhuang Weitao , Xu Congcong , Jin Ke , Chen Baofu , Tian Dan , Hiley Crispin , Onishi Hiroshi , Zhu Chengchu , Qiao Guibin
Published: May 2021

Background: Timing for intervention of small indeterminate pulmonary nodules has long been a topic of debate given the low incidence of malignancy and difficulty in obtaining a definite preoperative diagnosis. We sought to determine survival outcomes of surgical and non-surgical managements in non-small cell lung cancer (NSCLC) ≤8 mm, which may provide a reference for prospective decision-making for patients with suspected NSCLC.

Method: A total of 1,652 patients with Stage IA NSCLC ≤8 mm were identified from the Surveillance, Epidemiology, and End Results (SEER) database and categorized into surgery and non-surgery groups. Chi-square test, t-test and Mann-Whitney U test were used to compare the baseline characteristics between groups. Survival curves were depicted using Kaplan-Meier method and compared by log-rank test. Cox proportional hazard model was used for univariate and multivariate analyses. Adjustment of confounding factors between groups was performed by propensity score matching.

Results: The surgery and non-surgery groups included 1,438 and 208 patients, respectively. Patients in surgery group demonstrated superior survival outcome than patients in non-surgery group both before [overall survival (OS): HR, 16.22; 95% CI, 11.48–22.91, p < 0.001; cancer-specific survival (CSS): HR, 49.6; 95% CI, 31.09–79.11, p < 0.001] and after (OS: HR, 3.12; 95% CI, 2.40–4.05, p < 0.001; CSS: HR, 3.85; 95% CI, 2.74–5.40, p < 0.001) propensity score matching. The 30-day mortality rates were 3.1 and 12.0% in surgery and non-surgery groups, respectively. Multivariate analysis suggested age, sex, race, tumor size, grade, pathological stage were all independent prognostic factors in patients with ≤8 mm NSCLC. A comparison of surgical resections revealed a survival superiority of lobectomy over sub-lobectomy. In terms of CSS, no statistically significant difference was found between segmentectomy and wedge resection.

Conclusion: The current SEER database showed better prognosis of surgical resection than non-surgical treatment in patients with ≤8 mm NSCLC. However, the factors that should be essentially included in the proper propensity-matched analysis, such as comorbidity, cardiopulmonary function and performance status were unavailable and the true superiority or inferiority should be examined further by ongoing randomized trial, especially comparing surgery and stereotactic body irradiation.
The Role of Sperm Morphology Standards in the Laboratory Assessment of Bull Fertility in Australia
Authors: Perry V.E.A.
Published: May 2021

The lack of standardization in the laboratory assessment of semen questions the reliability of semen analysis, and makes meaningful interpretation of these evaluations impossible. We herein describe a standardization program for morphology assessment currently in place in Australia and outline the methods used, both for the categorization of the abnormalities, including newly described abnormalities, and those that permit standardized microscopic assessment between laboratories.
Value Co-Creation between Public Service Organizations and the Private Sector: An Organizational Capabilities Perspective
Authors: Rösler Jonathan , Söll Tobias , Hancock Louise , Friedli Thomas
Published: May 2021

Theoretically based on public service logic (PSL), this paper explores the barriers and capabilities surrounding public sector value co-creation with private sector organizations in the context of digital transformation. Specifically, value co-creation efforts at a large public transport and rail infrastructure provider are examined from multiple perspectives using an exploratory case study approach. Based on qualitative interview data, six barriers that hinder value co-creation in public service ecosystems and five corresponding organizational capabilities required to overcome them are identified. The study contributes to the field of public management research by shedding light on the concept of value co-creation in public-private sector digitalization collaborations.
Contrasting Responses of Guar Genotypes Shed Light on Multiple Component Traits of Salinity Tolerance Mechanisms
Authors: Sandhu Devinder , Pallete Andrew , Pudussery Manju V. , Grover Kulbhushan K.
Published: May 2021

Guar (Cyamopsis tetragonoloba (L.) Taub.) is a legume crop, and gum derived from its seeds has various industrial applications. Due to its tolerance to various abiotic stresses, guar can be grown under water-deficit or high-salinity conditions. In this investigation, four diverse guar genotypes that performed at a similar level in field conditions were evaluated in a salinity experiment in the greenhouse lysimeter system. Based on the salt tolerance index (STI) for shoot biomass, root biomass, shoot length, and root length, Matador and PI 268229 were classified as salt-tolerant, and PI 340261 and PI 537281 as salt-sensitive. Leaf Na concentrations were 4- to 5.5-fold higher, and leaf Cl concentrations were 1.6- to 1.9-fold higher in salt-sensitive lines than salt-tolerant lines under salinity. The strong associations between the leaf K concentrations under salinity compared to the control (K-salinity/K-control) ratio and STI for stem and root length advocate higher importance of K-salinity/K-control than total leaf K concentrations. The expression analyses of genes involved in Na+ and Cl− transport revealed the importance of different component traits of salinity tolerance mechanisms, such as the exclusion of Na+/Cl− from the root, sequestration of Cl− in root vacuoles, retrieval of Na+/Cl− from xylem during salinity stress, root-to-shoot Na+/Cl− translocation, and K+-Na+ homeostasis.
A State-of-the-Art Analysis of Obstacle Avoidance Methods from the Perspective of an Agricultural Sprayer UAV’s Operation Scenario
Authors: Ahmed Shibbir , Qiu Baijing , Ahmad Fiaz , Kong Chun-Wei , Xin Huang
Published: May 2021

Over the last decade, Unmanned Aerial Vehicles (UAVs), also known as drones, have been broadly utilized in various agricultural fields, such as crop management, crop monitoring, seed sowing, and pesticide spraying. Nonetheless, autonomy is still a crucial limitation faced by the Internet of Things (IoT) UAV systems, especially when used as sprayer UAVs, where data needs to be captured and preprocessed for robust real-time obstacle detection and collision avoidance. Moreover, because of the objective and operational difference between general UAVs and sprayer UAVs, not every obstacle detection and collision avoidance method will be sufficient for sprayer UAVs. In this regard, this article seeks to review the most relevant developments on all correlated branches of the obstacle avoidance scenarios for agricultural sprayer UAVs, including a UAV sprayer’s structural details. Furthermore, the most relevant open challenges for current UAV sprayer solutions are enumerated, thus paving the way for future researchers to define a roadmap for devising new-generation, affordable autonomous sprayer UAV solutions. Agricultural UAV sprayers require data-intensive algorithms for the processing of the images acquired, and expertise in the field of autonomous flight is usually needed. The present study concludes that UAV sprayers are still facing obstacle detection challenges due to their dynamic operating and loading conditions.
Mid-Term Effects of Conservative Soil Management and Fruit-Zone Early Leaf Removal Treatments on the Performance of Nerello Mascalese (Vitis vinifera L.) Grapes on Mount Etna (Southern Italy)
Authors: Nicolosi Elisabetta , Iovino Valeria , Distefano Gaetano , Di Guardo Mario , La Malfa Stefano , Gentile Alessandra , Palliotti Alberto , Las Casas Giuseppina , Ferlito Filippo
Published: May 2021

The aim of this study was to analyze the mid-term influence of minimum tillage (MT) combined with early leaf removal (ELR) on the performance of the black grapevine variety Nerello Mascalese (Vitis vinifera L.). The research was carried out from 2015 to 2018 in a vineyard located in the Mount Etna area, East Sicily, Southern Italy. An average of about 8000 cm2 of leaves were removed from the ELR vines, corresponding to a canopy defoliation of 44% in 2017 and 30% in 2018. As for the stem water potential of both primary and lateral leaves, ELR-MT treatment showed the best performance (up to −0.4 MPa) among the four combinations. In 2017, no differences in yield/vine were found among treatments, whereas in 2018 the yield was significantly lower in ELR treatments. Among the different treatments performed, the ELR-MT showed the best results both in terms of physiological plant parameters (water status and photosynthetic activity) and fruit quality (higher concentration of sugars and total anthocyanins and polyphenols). ELR-MT treatment is also associated with a lower incidence of tillage operations from four to only one per year (−60–70 h/ha). In contrast to what was observed in the first 3 years of application (i.e., from 2015 to 2017), yield was heavily penalized during the fourth year of application (2018), probably due to the excessive depletion of nutritional reserves, the reduction of bud fertility and the unfavorable meteorological condition. For these reasons, the ELR-MT combination might require an interruption every 2–3 years of application.
Forage Potential of Non-Native Guinea Grass in North African Agroecosystems: Genetic, Agronomic, and Adaptive Traits
Authors: Benabderrahim Mohamed Ali , Elfalleh Walid
Published: May 2021

Guinea grass (Panicum maximum Jacq., renamed Megathyrsus maximus Jacq.) is a native forage plant in Africa of great economic value, but it was introduced in almost all tropical countries as a source of animal forage. Over the last decade, it was introduced in North arid regions of Africa (Morocco, Algeria, Tunisia, Libya, and Egypt) through authorized and unauthorized ways. It has two reproduction modes through sexual and apomictic ways. Besides its ability to provide high nutritive forage, guinea grass could affect the oases agroecosystems diversity due to its genetic aspects (apomixis and autotetraploidy) and eco-physiological traits (allelopathy effect and resistance to abiotic stress). That is why a review of genetic and eco-physiologic aspects of guinea grass is essential to investigate its potential introduction and management in new regions, particularly in arid and semiarid zones. In this paper, we review the most important traits of this plant that should be considered (polyploidy, apomixis, allelopathic effect, drought and salinity resistance, and invasion) for the potential success of guinea grass in integrated systems of forage/livestock.
The Effect of the Proportion of Adjacent Non-Crop Vegetation on Plant and Invertebrate Diversity in the Vineyards of the South Moravian Region
Authors: Ragasová Lucia , Kopta Tomáš , Winkler Jan , Šefrová Hana , Pokluda Robert
Published: May 2021

Increasing vulnerability of crops to pests and diseases, problems with soil erosion, a decline in biodiversity and a number of other negative impacts caused by agricultural intensification and monocultural production have been the subjects of many studies in recent decades. Today, cover cropping has become a promising practice to defuse these negative impacts, and it is emerging in many wine-producing regions, including the Czech Republic. However, the importance of permanent natural and semi-natural habitats in agricultural production should not be neglected. In this study, the effect of adjacent non-crop vegetation on plant and insect diversity was evaluated. The highest plant species richness of inter-row vegetation was found in vineyards with a high proportion (>40%) of non-crop vegetation within a 500-m radius. Regarding the agricultural impact of inter-row vegetation, the high proportion of non-crop vegetation could have been related to the higher presence of opportunistic and non-harmful weeds, compared with the presence of dangerous weed species. The number of insect families present in inter-rows was probably affected more by the vegetation coverage rate than by the proportion of adjacent non-crop vegetation. However, the occurrence of the Hymenoptera species, often representing beneficial organisms, was related to localities with a high proportion of adjacent non-crop vegetation.
Special Issue “Pollinator Diversity and Pollination in Agricultural Systems”
Authors: Drummond Francis
Published: May 2021

Animal-mediated pollination and the subsequent fertilization of plants is the most important eco-physiological process that occurs during the production of many fruit, vegetable, and nut crops [...]
Use Bottom Sediment to Agriculture—Effect on Plant and Heavy Metal Content in Soil
Authors: Kazberuk Witold , Szulc Wiesław , Rutkowska Beata
Published: May 2021

Removing bottom sediment from water reservoirs and rivers can, on the one hand, be an effective method to restore lakes, and on the other—be used for plant production, ensuring the recycling of nutrients. The aim of this research was to evaluate the possibilities of using various types of bottom sediment and its impact on heavy metal content in soil and plants. For this purpose, a pot experiment was carried out using white mustard (Sinapis alba) as a test plant. The total content of heavy metals (Cd, Cu, Zn, Pb) was determined in soil and plant. The addition of all types of bottom sediment increased heavy metal content in the soil. The results indicate that adding bottom sediment resulted in a significant increase in plant yield in comparison to the control. The highest yield as a result of direct effect was obtained for a combination with a 5% addition of dam sediment, while as a result of residual effect, the highest yield was achieved for a mixture with a 10% addition of pond sediment. The values of the transfer factor (TF = Cplant/Csoil) indicate a high accumulation of zinc and low accumulation of lead in the plant.
Impact of Milk Thistle (Silybum marianum [L.] Gaertn.) Seeds in Broiler Chicken Diets on Rearing Results, Carcass Composition, and Meat Quality
Authors: Janocha Alina , Milczarek Anna , Pietrusiak Daria
Published: May 2021

The studies aimed to evaluate the impact of milk thistle seeds in broiler chicken feed rations on rearing results, carcass composition and meat quality. The experiment involved 120 broiler chickens randomly allocated to three equinumerous groups (C, MT02, MT23). Each group was divided into five subgroups of eight chickens each. Over the first 21 days of life the birds were fed starter rations, and over the following 21 days received grower rations. Chicken starter/grower diets in groups MT02 and MT23 were supplemented with ground seeds of milk thistle in the amount of 0/2% (MT02) and 2/3% (MT23). It was demonstrated that Silybum marianum added to feed rations over the whole rearing period (group MT23) increased above 3% the birds’ body weight on rearing day 42. (p < 0.05) and decreased about 7% the feed conversion ratio (p < 0.05) in comparison to group C. No effect of feeding on the carcass composition was observed, including on muscularity and fattening grade, although diets containing milk thistle reduced (by 15% and 19% in group MT02 and MT23, respectively) the content of crude fat in chicken leg muscles (p < 0.05). The highest (p < 0.05) content of polyunsaturated fatty acids (PUFA) was determined in the breast (38.06%) and leg (37.63%) muscles of chicken receiving feed rations containing Silybum marianum throughout the rearing period. No effect of nutrition on the evaluated physical properties of muscles was observed, except on the decrease in lightness color (L*) and increase in values a* and C as well as a decrease of water holding capacity of the breast muscles. It was found that Silybum marianum in chicken diets had a positive effect on the evaluated meat flavor characteristics of the muscles. To sum up, based on the study results, including ground seeds of milk thistle in broiler chickens nutrition can be recommended in the amount of 2/3% in starter/grower diets, respectively.
Effect of Oats and Wheat Genotype on In Vitro Gas Production Kinetics of Straw
Authors: Peñailillo Karen A. , Aedo María Fernanda , Scorcione María Carolina , Mathias Mónica L. , Jobet Claudio , Vial Manuel , Lobos Iris A. , Saldaña Rodolfo C. , Escobar-Bahamondes Paul , Etcheverría Paulina , Ungerfeld Emilio M.
Published: May 2021

Increases in cereals grain yield in the last decades have increased the accumulation of straw on the soil after harvest. Farmers typically open burn the straw to prepare the soil for the next crop, resulting in pollution, emission of greenhouse gases, erosion, loss of soil organic matter, and wildfires. An alternative is feeding straw to ruminants, but straw nutritive value is limited by its high content of lignocellulose and low content of protein. Cereal breeding programs have focused on improving grain yield and quality and agronomic traits, but little attention has been paid to straw nutritive value. We screened straw from 49 genotypes of oats and 24 genotypes of wheat from three cereal breeding trials conducted in Chile for in vitro gas production kinetics. We found moderate effects of the genotype on gas production at 8, 24, and 40 h of incubation, and on the maximum extent and rate of gas production. Gas production was negatively associated with lignin and cellulose contents and not negatively associated with grain yield and resistance to diseases and lodging. Effects observed in vitro need to be confirmed in animal experiments before gas production kinetics can be adopted to identify cereal genotypes with more digestible straw.
Attenuation of Aeromonas hydrophila Infection in Carassius auratus by YtnP, a N-acyl Homoserine Lactonase from Bacillus licheniformis T-1
Authors: Peng Mengfan , Tong Wentao , Zhao Zhen , Xiao Ling , Wang Zhaoyue , Liu Xuanming , He Xuanhao , Song Zengfu
Published: May 2021

In this experiment, the quorum quenching gene ytnP of Bacillus licheniformis T-1 was cloned and expressed, and the effect against infection of Aeromonas hydrophila ATCC 7966 was evaluated in vitro and vivo. The BLAST results revealed a 99% sequence identity between the ytnP gene of T-1 and its homolog in B.subtilis sub sp. BSP1, and the dendroGram showed that the similarity in the YtnP protein in T-1 was 100% in comparison with B.subtilis 3610, which was categorized as the Aidc cluster of the MBL family. The AHL lactonase activity of the purified YtnP was detected as 1.097 ± 0.7 U/mL with C6-HSL as the substrate. Otherwise, purified YtnP protein could significantly inhibit the biofilm formation of A.hydrophila ATCC 7966 with an inhibition rate of 68%. The MIC of thiamphenicol and doxycycline hydrochloride against A. hydrophila reduced from 4 μg/mL and 0.5 μg/mL to 1 μg/mL and 0.125 μg/mL, respectively, in the presence of YtnP. In addition, YtnP significantly inhibited the expression of five virulence factors hem, ahyB, ast, ep, aerA of A. hydrophila ATCC 7966 as well (p < 0.05). The results of inhibition on virulence showed a time-dependence tendency, while the strongest anti-virulence effects were within 4–24 h. In vivo, when the YtnP protein was co-injected intraperitoneally with A. hydrophila ATCC 7966, it attenuated the pathogenicity of A. hydrophila and the accumulated mortality was 27 ± 4.14% at 96 h, which was significantly lower than the average mortality of 78 ± 2.57% of the Carassius auratus injected with 108 CFU/mL of A. hydrophila ATCC 7966 only (p < 0.001). In conclusion, the AHL lactonase in B. licheniformis T-1 was proven to be YtnP protein and could be developed into an agent against infection of A. hydrophila in aquaculture.
Essential Oils from Two Apiaceae Species as Potential Agents in Organic Crops Protection
Authors: Merad Nadjiya , Andreu Vanessa , Chaib Slimane , de Carvalho Augusto Ronaldo , Duval David , Bertrand Cédric , Boumghar Yacine , Pichette André , Djabou Nassim
Published: May 2021

Chemical composition and herbicidal, antifungal, antibacterial and molluscicidal activities of essential oils from Choukzerk, Eryngium triquetrum, and Alexander, Smyrnium olusatrum, from western Algeria were characterized. Capillary GC-FID and GC/MS were used to investigate chemical composition of both essential oils, and the antifungal, antibacterial, molluscicidal and herbicidal activities were determined by % inhibition. Collective essential oil of E. triquetrum was dominated by falcarinol (74.8%) and octane (5.6%). The collective essential oil of S. olusatrum was dominated by furanoeremophilone (31.5%), furanodiene+curzurene (19.3%) and (E)-β-caryophyllene (11%). The E. triquetrum oil was tested and a pure falcarinol (99%) showed virtuous herbicidal and antibacterial activities against potato blackleg disease, Pectobacterium atrosepticum, and Gram-negative soil bacterium, Pseudomonas cichorii (85 and 100% inhibition, respectively), and high ecotoxic activity against brine shrimp, Artemia salina, and the freshwater snail, Biomphalaria glabrata, with an IC50 of 0.35 µg/mL and 0.61 µg/mL, respectively. Essential oil of S. olusatrum showed interesting antibacterial and ecotoxic activity and good herbicidal activity against watercress seeds, Lepidium sativum (74% inhibition of photosynthesis, 80% mortality on growth test on model watercress), while the furanoeremophilone isolated from the oil (99% pure) showed moderate herbicidal activity. Both oils showed excellent antifungal activity against Fusarium. Both oils and especially falcarinol demonstrated good potential as new biocontrol agents in organic crop protection.
In Vitro Selection of High-Level Beta-Lactam Resistance in Methicillin-Susceptible Staphylococcus aureus
Authors: Gostev Vladimir , Kalinogorskaya Olga , Ivanova Ksenia , Kalisnikova Ekaterina , Lazareva Irina , Starkova Polina , Sidorenko Sergey
Published: May 2021

Selective pressure of beta-lactams is thought to be responsible for mutation selection in methicillin-susceptible Staphylococcus aureus (MSSA). We used next-generation sequencing to compare the genomes of beta-lactamase-positive (SA0707) and -negative (SA0937) MSSA isolates with their derivatives obtained after selection with oxacillin, ceftaroline, or meropenem. Selection with oxacillin and ceftaroline caused a rapid and significant (6–8 times) increase in the minimum inhibitory concentration (MICs) of oxacillin, penicillin, amoxicillin/clavulanate, and ceftaroline against the derivatives of both isolates, associated with growth impairment. Selection with meropenem caused a limited increase in the MICs of all beta-lactams against both isolates. During the initial stages of selection (after 5–15 passages), mutations were detected only in some reads, which indicated the heterogeneity of the population; however, during the later stages, either the population reversed to the wild type or fixation of the mutation was observed in the entire population. Selection with different beta-lactams caused diverse mutational events, but common mutations were detected in gdpP, all penicillin-binding proteins, cell wall regulators (vraST, graR), and deletions in the promoter region of pbp4. Therefore, the disk diffusion test with cefoxitin does not reveal resistance associated with these mechanisms in some cases, which can lead to the failure of beta-lactam therapy.
Nrf2 Regulates Anti-Inflammatory A20 Deubiquitinase Induction by LPS in Macrophages in Contextual Manner
Authors: Potteti Haranatha R. , Venkareddy Lalith K. , Noone Patrick M. , Ankireddy Aparna , Tamatam Chandramohan R. , Mehta Dolly , Tiruppathi Chinnaswamy , Reddy Sekhar P.
Published: May 2021

The aberrant regulation of inflammatory gene transcription following oxidant and inflammatory stimuli can culminate in unchecked systemic inflammation leading to organ dysfunction. The Nrf2 transcription factor dampens cellular stress and controls inflammation by upregulating antioxidant gene expression and TNFα-induced Protein 3 (TNFAIP3, aka A20) deubiquitinase by controlling NF-kB signaling dampens tissue inflammation. Here, we report that Nrf2 is required for A20 induction by inflammatory stimuli LPS in monocyte/bone marrow derived macrophages (MDMΦs) but not in lung-macrophages (LDMΦs). LPS-induced A20 expression was significantly lower in Nrf2−/− MDMΦs and was not restored by antioxidant supplementation. Nrf2 deficiency markedly impaired LPS-stimulated A20 mRNA expression Nrf2−/− MDMΦs and ChIP assays showed Nrf2 enrichment at the promoter Nrf2−/− MDMΦs upon LPS stimulation, demonstrating that Nrf2 directly regulates A20 expression. Contrary to MDMΦs, LPS-stimulated A20 expression was not largely impaired in Nrf2−/− LDMΦs ex vivo and in vivo and ChIP assays showed lack of increased Nrf2 binding at the A20 promoter in LDMΦ following LPS treatment. Collectively, these results demonstrate a crucial role for Nrf2 in optimal A20 transcriptional induction in macrophages by endotoxin, and this regulation occurs in a contextual manner.
Distribution of Polyphenolic and Isoprenoid Compounds and Biological Activity Differences between in the Fruit Skin + Pulp, Seeds, and Leaves of New Biotypes of Elaeagnusmultiflora Thunb
Authors: Lachowicz-Wiśniewska Sabina , Kapusta Ireneusz , Stinco Carla M. , Meléndez-Martínez Antonio J. , Bieniek Anna , Ochmian Ireneusz , Gil Zygmunt
Published: May 2021

The purpose of this study was to determine the distribution of polyphenolic and isoprenoid compounds and organic acids in the fruit skin + pulp, seeds, and leaves of six new biotypes of Elaeagnus multiflora Thunb., as well as their in vitro biological potency. The polyphenols and isoprenoids were determined with UPLC-PDA-MS/MS (ultra-performance liquid chromatography coupled to photodiode array detection and electrospray ionization tandem mass spectrometry) and RRLC-MS/MS (rapid resolution liquid chromatography/tandem mass spectrometry) methods, the organic acid with HPLC-RID (high-performance liquid chromatography coupled to a Refractive Index Detector), and the antioxidant capacity using ABTS and FRAP assays. Enzymatic activity was established as the ability to inhibit α-amylase, α-glucosidase, and pancreatic lipase. Owing to such an effective technique, 88 compounds were recorded, with 17 polyphenolic compounds and 3 isoprenoids identified for the first time in the seeds and leaves of cherry silverberry. In total, 55 compounds were identified in the leaves, 36 in the seeds, and 31 in the fruit skin + pulp. The predominant polyphenol was polymeric procyanidin (66–95% of total polyphenolics), whereas the predominant isoprenoids were chlorophyll b and (all-E)-lycopene. The results of our work noted that there are significant differences in the profiles of several secondary metabolites between the analyzed parts of the plant, and depending on the need, the compounds can be used to develop different innovative food or cosmetic products.
Disinclusion of Palatally Impacted Canines with Surgical and Photobiomodulating Action of a Diode Laser: Case Series
Authors: Impellizzeri Alessandra , Horodynski Martina , De Stefano Adriana , Guercio-Monaco Elisabetta , Palaia Gaspare , Serritella Emanuela , Polimeni Antonella , Galluccio Gabriella
Published: May 2021

Background: The permanent maxillary canine is the most impacted tooth after third molars. There are many possible surgical techniques to approach impacted canines. The literature reports that high-intensity laser therapies (HILTs) can be used for opercolectomy of an impacted tooth. The aim of this study is to propose a new orthodontic-surgical approach using a laser for the disinclusion of palatally impacted canines. Methods: Nine patients presented maxillary primary canine persistence in the dental arch. Orthopanoramic X-ray and cone beam computed tomography (CBCT) showed the impaction of permanent maxillary canines. Surgical exposure was performed using a diode laser (Raffaello, DMT, Lissone, Italy, 980 nm + 645 nm). No orthodontic devices were applied for impacted tooth traction. Canine movement was monitored at 1, 8, 16 weeks post-surgery with photos and a CS3500 intraoral scanner (Carestream Dental, Atlanta USA) to evaluate their autonomous eruption. Results: No complications were observed. In all the cases, complete disimpaction of the treated canines was obtained in only four months. Conclusions: Impacted canine exposure with a diode laser has many advantages if compared with traditional surgery. The pre-orthodontic exposure and autonomous eruption of impacted canines provided simplified and predictable outcomes. The absence of traction and the reduced time for orthodontic treatment increased compliance during the orthodontic alignment.
Quantifying Sub-Elite Youth Football Weekly Training Load and Recovery Variation
Authors: Teixeira José E. , Forte Pedro , Ferraz Ricardo , Leal Miguel , Ribeiro Joana , Silva António J. , Barbosa Tiago M. , Monteiro António M.
Published: May 2021

Monitoring the training load in football is an important strategy to improve athletic performance and an effective training periodization. The aim of this study was two-fold: (1) to quantify the weekly training load and recovery status variations performed by under-15, under-17 and under-19 sub-elite young football players; and (2) to analyze the influence of age, training day, weekly microcycle, training and playing position on the training load and recovery status. Twenty under-15, twenty under-17 and twenty under-19 players were monitored over a 2-week period during the first month of the 2019–2020 competitive season. Global positioning system technology (GPS) was used to collect external training loads: total distance covered, average speed, maximal running speed, relative high-speed running distance, high metabolic load distance, sprinting distance, dynamic stress load, accelerations and decelerations. Internal training load was monitored using ratings of perceived exertion (RPE) and session rating of perceived exertion (sRPE). Recovery status was obtained using the total quality recovery (TQR) scale. The results show an age-related influence for external training load (p ≤ 0.001; d = 0.29–0.86; moderate to strong effect), internal training load (p ≤ 0.001, d = 0.12–0.69; minimum to strong effect) and recovery status (p ≤ 0.001, d = 0.59; strong effect). The external training load presented differences between training days (p < 0.05, d = 0.26–0.95; moderate to strong effect). The playing position had a minimum effect on the weekly training load (p < 0.05; d = 0.06–0.18). The weekly microcycle had a moderate effect in the TD (p < 0.05, d = 0.39), RPE (p < 0.05; d = 0.35) and sRPE (p < 0.05, d = 0.35). Interaction effects were found between the four factors analyzed for deceleration (F = 2.819, p = 0.017) and between inter-day, inter-week and age for total covered distance (F = 8.342, p = 0.008). This study provided specific insights about sub-elite youth football training load and recovery status to monitor training environments and load variations. Future research should include a longer monitoring period to assess training load and recovery variations across different season phases.
Numerical Simulation of KBKZ Integral Constitutive Equations in Hierarchical Grids
Authors: Bertoco Juliana , de Araújo Manoel S. B. , Leiva Rosalía T. , Sánchez Hugo A. C. , Castelo Antonio
Published: May 2021

In this work, we present the implementation and verification of HiGTree-HiGFlow solver (see for numerical simulation of the KBKZ integral constitutive equation. The numerical method proposed herein is a finite difference technique using tree-based grids. The advantage of using hierarchical grids is that they allow us to achieve great accuracy in local mesh refinements. A moving least squares (MLS) interpolation technique is used to adapt the discretization stencil near the interfaces between grid elements of different sizes. The momentum and mass conservation equations are solved by an implicit method and the Chorin projection method is used for decoupling the velocity and pressure. The Finger tensor is calculated using the deformation fields method and a three-node quadrature formula is used to derive an expression for the integral tensor. The results of velocity and stress fields in channel and contraction-flow problems obtained in our simulations show good agreement with numerical and experimental results found in the literature.
Why Do Tethered-Bilayer Lipid Membranes Suit for Functional Membrane Protein Reincorporation?
Authors: Girard-Egrot Agnès P. , Maniti Ofelia
Published: May 2021

Membrane proteins (MPs) are essential for cellular functions. Understanding the functions of MPs is crucial as they constitute an important class of drug targets. However, MPs are a challenging class of biomolecules to analyze because they cannot be studied outside their native environment. Their structure, function and activity are highly dependent on the local lipid environment, and these properties are compromised when the protein does not reside in the cell membrane. Mammalian cell membranes are complex and composed of different lipid species. Model membranes have been developed to provide an adequate environment to envisage MP reconstitution. Among them, tethered-Bilayer Lipid Membranes (tBLMs) appear as the best model because they allow the lipid bilayer to be decoupled from the support. Thus, they provide a sufficient aqueous space to envisage the proper accommodation of large extra-membranous domains of MPs, extending outside. Additionally, as the bilayer remains attached to tethers covalently fixed to the solid support, they can be investigated by a wide variety of surface-sensitive analytical techniques. This review provides an overview of the different approaches developed over the last two decades to achieve sophisticated tBLMs, with a more and more complex lipid composition and adapted for functional MP reconstitution.
A Redox-Neutral, Two-Enzyme Cascade for the Production of Malate and Gluconate from Pyruvate and Glucose
Authors: Mandair Ravneet , Karagoz Pinar , Bill Roslyn M.
Published: May 2021

A triple mutant of NADP(H)-dependent malate dehydrogenase from thermotolerant Thermococcus kodakarensis has an altered cofactor preference for NAD+, as well as improved malate production compared to wildtype malate dehydrogenase. By combining mutant malate dehydrogenase with glucose dehydrogenase from Sulfolobus solfataricus and NAD+/NADH in a closed reaction environment, gluconate and malate could be produced from pyruvate and glucose. After 3 h, the yield of malate was 15.96 mM. These data demonstrate the feasibility of a closed system capable of cofactor regeneration in the production of platform chemicals.
Systematic Review of Anomaly Detection in Hyperspectral Remote Sensing Applications
Authors: Racetin Ivan , Krtalić Andrija
Published: May 2021

Hyperspectral sensors are passive instruments that record reflected electromagnetic radiation in tens or hundreds of narrow and consecutive spectral bands. In the last two decades, the availability of hyperspectral data has sharply increased, propelling the development of a plethora of hyperspectral classification and target detection algorithms. Anomaly detection methods in hyperspectral images refer to a class of target detection methods that do not require any a-priori knowledge about a hyperspectral scene or target spectrum. They are unsupervised learning techniques that automatically discover rare features on hyperspectral images. This review paper is organized into two parts: part A provides a bibliographic analysis of hyperspectral image processing for anomaly detection in remote sensing applications. Development of the subject field is discussed, and key authors and journals are highlighted. In part B an overview of the topic is presented, starting from the mathematical framework for anomaly detection. The anomaly detection methods were generally categorized as techniques that implement structured or unstructured background models and then organized into appropriate sub-categories. Specific anomaly detection methods are presented with corresponding detection statistics, and their properties are discussed. This paper represents the first review regarding hyperspectral image processing for anomaly detection in remote sensing applications.
A Performance Analysis of Internet of Things Networking Protocols: Evaluating MQTT, CoAP, OPC UA
Authors: Silva Daniel , Carvalho Liliana I. , Soares José , Sofia Rute C.
Published: May 2021

IoT data exchange is supported today by different communication protocols and different protocolar frameworks, each of which with its own advantages and disadvantages, and often co-existing in a way that is mandated by vendor policies. Although different protocols are relevant in different domains, there is not a protocol that provides better performance (jitter, latency, energy consumption) across different scenarios. The focus of this work is two-fold. First, to provide a comparison of the different available solutions in terms of protocolar features such as type of transport, type of communication pattern support, security aspects, including Named-data networking as relevant example of an Information-centric networking architecture. Secondly, the work focuses on evaluating three of the most popular protocols used both in Consumer as well as in Industrial IoT environments: MQTT, CoAP, and OPC UA. The experimentation has been carried out first on a local testbed for MQTT, COAP and OPC UA. Then, larger experiments have been carried out for MQTT and CoAP, based on the large-scale FIT-IoT testbed. Results show that CoAP is the protocol that achieves across all scenarios lowest time-to-completion, while OPC UA, albeit exhibiting less variability, resulted in higher time-to-completion in comparison to CoAP or MQTT.
Multitask Learning with Knowledge Base for Joint Intent Detection and Slot Filling
Authors: He Ting , Xu Xiaohong , Wu Yating , Wang Huazhen , Chen Jian
Published: May 2021

Intent detection and slot filling are important modules in task-oriented dialog systems. In order to make full use of the relationship between different modules and resource sharing, solving the problem of a lack of semantics, this paper proposes a multitasking learning intent-detection system, based on the knowledge-base and slot-filling joint model. The approach has been used to share information and rich external utility between intent and slot modules in a three-part process. First, this model obtains shared parameters and features between the two modules based on long short-term memory and convolutional neural networks. Second, a knowledge base is introduced into the model to improve its performance. Finally, a weighted-loss function is built to optimize the joint model. Experimental results demonstrate that our model achieves better performance compared with state-of-the-art algorithms on a benchmark Airline Travel Information System (ATIS) dataset and the Snips dataset. Our joint model achieves state-of-the-art results on the benchmark ATIS dataset with a 1.33% intent-detection accuracy improvement, a 0.94% slot filling F value improvement, and with 0.19% and 0.31% improvements respectively on the Snips dataset.
Computational Fluid Dynamic Analysis of Flow Rate Performance of a Small Piezoelectric-Hydraulic Pump
Authors: Anh Phuc Nguyen , Bae Jae-Sung , Hwang Jai-Hyuk
Published: May 2021

This paper investigates the transient flow rate performance of small piezoelectric-hydraulic pumps. In a previous study, a small piezoelectric hydraulic pump was designed and developed to be applicable to the braking systems of small- and medium-sized UAVs (unmanned aerial vehicles). To this end, a thin plate spring check valve was designed in order to effectively discharge the flow in a single direction. The flow rate of the piezoelectric-hydraulic pump is an important criterion for evaluating pump efficiency. Therefore, a study on the parameters affecting such a flow rate is necessary to enhance the efficiency of piezoelectric hydraulic pumps used in brake systems. This study on small piezoelectric-hydraulic pumps is performed to accurately predict the flow rate using a CFD (Computational Fluid Dynamics) tool. In other words, an unsteady CFD method is applied to model the transient flow rate characteristics and the internal flow field of the fluid. The visualization of the internal flow field is evaluated for a better understanding of the flow fields inside the pump. Moreover, this work also illustrates the detailed motion of both the inlet and outlet check valves during the pump operation that fully reflects the phase shift between the check valves and the piston motion, all of which affect the flow rate performance of the pump. An experiment of flow rate characteristics was conducted on a designed piezoelectric-hydraulic pump, which verifies the validity of the CFD results.
Effect of Red Cabbage Sprouts Treating with Organic Acids on the Content of Polyphenols, Antioxidant Properties and Colour Parameters
Authors: Najman Katarzyna , Sadowska Anna
Published: May 2021

In recent years, there has been a great deal of consumer interest in consuming vegetables in the form of sprouts, characterized by high nutritional value. The disadvantage of sprouts is the loss of bioactive compounds during storage and the relatively short shelf life, due to the fact that they are a good medium for microorganisms, especially yeasts and molds. The aim of the study was to compare the content of polyphenols, antioxidant properties, color and microbiological quality of red cabbage sprouts preserved by the use of mild organic acids: Citric, ascorbic, lactic, acetic and peracetic. In the study, the content of polyphenols and antioxidant properties of sprouts was examined using the spectrophotometric method, instrumental color measurement was done using an “electronic eye” and the content of mold, yeast and the total number of mesophilic microorganisms was determined using the plate inoculation method. Taking into account the content of polyphenols and the antioxidant potential of sprouts, it was found that the addition of all organic acids contributed to the preservation of the tested compounds during their 14-day storage under refrigerated conditions, depending on the type of organic acid used, from 71 to 86% for polyphenols and from 75 to 96% for antioxidant properties. The best results were obtained by treating the sprouts with peracetic acid and ascorbic acid, respectively, at a concentration of 80 ppm and 1%. The conducted research on the possibility of extending the storage life and preserving the bioactive properties of fresh sprouts showed that the use of peracetic acid in the form of an aqueous solution during pre-treatment allows to reduce the content of microorganisms by one logarithmic order. Ascorbic acid did not reduce the content of microorganisms in the sprout samples tested. Considering the content of bioactive ingredients, as well as the microbiological quality of fresh sprouts, it can be said that there is a great need to use mild organic acids during the pre-treatment of sprouts in order to maintain a high level of health-promoting ingredients during their storage, which may also contribute to their prolongation durability.
Prediction Method of Underwater Acoustic Transmission Loss Based on Deep Belief Net Neural Network
Authors: Zhao Yihao , Wang Maofa , Xue Huanhuan , Gong Youping , Qiu Baochun
Published: May 2021

The prediction of underwater acoustic transmission loss in the sea plays a key role in generating situational awareness in complex naval battles and assisting underwater operations. However, the traditional classical underwater acoustic transmission loss models do not consider the regional hydrological elements, and the performance of underwater acoustic transmission loss prediction under complex environmental conditions in a wide range of sea areas is limited. In order to solve this problem, we propose a deep learning-based underwater acoustic transmission loss prediction method. First, we studied the application domains of typical underwater acoustic transmission loss models (ray model, normal model, fast field program model, parabolic equation model), analyzed the constraint rules of its characteristic parameters, and constructed a dataset according to the rules. Then, according to the characteristics of the dataset, we built a DBN (deep belief net) neural network model and used DBN to train and learn the dataset. Through the DBN method, the adaptation and calculation of the underwater acoustic transmission loss model under different regional hydrological elements were carried out in a simulation environment. Finally, the new method was verified with the measured transmission loss data of acoustic sea trials in a certain sea area. The results show that the RMSE error between the underwater acoustic transmission loss calculated by the new method and the measured data was less than 6.5 dB, the accuracy was higher than that of the traditional method, and the prediction speed was faster, the result was more accurate, and had a wide range of adaptability in complex seas.
Deep Eutectic Solvents for the Extraction of Bioactive Compounds from Natural Sources and Agricultural By-Products
Authors: Socas-Rodríguez Bárbara , Torres-Cornejo Monica Vanessa , Álvarez-Rivera Gerardo , Mendiola Jose A.
Published: May 2021

In this work, a review about the applicability of eutectic solvents, mainly deep eutectic solvents (DES) and natural deep eutectic solvents (NADES), for the extraction of bioactive compounds from natural products has been carried out. These alternative solvents have shown not only to have high extraction yields but also to be environmentally friendly, exhibiting very low or almost no toxicity, compared to conventional organic solvents. The last trends and main extraction methods that have been most widely used in studies using these emerging solvents have been reviewed, as well as the varied natural sources in which they have been used, including agro-food by-products. Besides the toxicity, biodegradability of these solvents is reviewed. Likewise, different reported bioactivity tests have been included, in which extracts obtained with these ecological solvents have been tested from antioxidant activity analysis to in vivo studies with rats, through in vitro cytotoxicity tests.
Estimation of Dose Enhancement for Inhomogeneous Distribution of Nanoparticles: A Monte Carlo Study
Authors: Abolaban Fouad , Taha Eslam , Alhawsawi Abdulsalam , Djouider Fathi , Banoqitah Essam , Nisbet Andrew
Published: May 2021

High atomic number nanoparticles are of increasing interest in radiotherapy due to their significant positive impact on the local dose applied to the treatment site. In this work, three types of metal nanoparticles were utilized to investigate their dose enhancement based on the GATE Monte Carlo simulation tool. Gold, gadolinium, and silver were implanted at three different concentrations to a 1 cm radius sphere to mimic a cancerous tumor inside a 10 × 10 × 30 cm3 water phantom. The innermost layer of the tumor represents a necrotic region, where the metal nanoparticles uptake is assumed to be zero, arising from hypoxic conditions. The nanoparticles were defined using the mixture technique, where nanoparticles are added to the chemical composition of the tumor. A directional 2 × 2 cm2 monoenergetic photon beam was used with several energies ranging from 50 keV to 4000 keV. The dose enhancement factor (DEF) was measured for all three metal nanoparticles under all beam energies. The maximum DEF was ~7 for silver nanoparticles with the 50 keV beam energy at the highest nanoparticle concentration of 30 mg/g of water. Gold followed the same trend as it registered the highest DEF at the 50 keV beam energy with the highest concentration of nanoparticles at 30 mg/g, while gadolinium registered the highest at 100 keV.