bluuebunny/crossref_metadata_2025 huggingface dataset:
Nasalance Scores for Normal Persian-Speaking Girls
Authors: Sadeghi Saba , Derakhshandeh Fatemeh , Abdali Hossein , Rezaei Parisa
Published: July 2021

Background: Obtaining normative nasalance scores is essential in the process of assessing and treating resonance disorders. The purpose of this study was to obtain nasalance scores in Persian-speaking girls aged 4-6 years and investigate the age-related differences. Materials and Methods: All participants (n=40) were screened to identify any overt problems relating to resonance, hearing, voice quality, or speech and language skills. The mean nasalance scores were obtained from normal-speaking girls during the repetition of the Persian version of the Simplified Nasometric Assessment Procedures (SNAP) test subtests. The Nasometer II (model 6450) was used to obtain the nasalance scores. Results: The mean nasalance score for the SNAP test subtests was obtained. Group mean and standard deviation (SD) nasalance scores of girls for oral and nasal sentences were 12.59±3.74 and 50.52±6.39, respectively. There was no significant difference between age groups (4, 5 and 6 years old) (P<0.05). Conclusion: Our results provided normative nasalance scores based on the SNAP test that can be used for the evaluation and treatment of resonance problems in Persian-speaking girls.
The Effect of 12 Weeks of Aerobic Exercise on Anxiety and Happiness of Female High School Students
Authors: babaei solmaz , Jamali Qarakhanlou Bahram
Published: July 2021

Background and Objectives: One of the most important factors in mental health is cultivating happiness and reducing anxiety in students. Therefore, the aim of this study was to investigate the effect of 12 weeks of aerobic exercise on anxiety and happiness of female high school students. Material and Methods: The present study was a quasi-experimental study and was performed as a pretest-posttest with a control group. The statistical population of the study consisted of all female high school students in Urmia city of Iran, of whom 40 vulentiarly agreed to participate in the study and were randomly divided into two groups of 20 experimental and control. For the experimental group, aerobic exercises were performed for 12 weeks, three sessions per week and and each session takes 45 to 60 minutes. The Oxford Happiness and Spielberger Anxiety Questionnaire were used in this study. Data analysis was performed by analysis of covariance using spss22 software. Results: The results showed a significant effect of aerobic exercise on promoting happiness variables and reducing anxiety in students (p < 0.05). Conclusion: Aerobic exercise improves students' cognitive functions, so using this approach has been recommended in order to promote students' mental health.
Highlighting the Relevance of Motivation and Job Satisfaction of Nurses in Ghanaian Hospitals
Authors: Botchwey Charles Owusu-Aduomi
Published: July 2021

Background: Provision of quality and accessible health services comes with its challenges. Therefore, the need to highlight how motivation and job satisfaction can impact nurses’ performances at the Korle-Bu teaching hospital. Objectives: The research aimed to practically highlight nurses’ motivation and job satisfaction by identifying what constituted motivation packages of nurses and how the work they did could give them satisfaction at the Korle-Bu teaching hospital. Methods: The research employed a qualitative method with a case study design. In-depth interviews and focus group discussions (data triangulation) were used for primary data collection. A total of 40 respondents, including ten key informants, were selected using the purposive and convenience sampling techniques. Results: The research found that the recognition of needs of nurses, improved remunerations, enhanced conditions of service of the nurses’, regular payment of nurses’ salaries’, the institution of study leave with pay policy, among others, were considered as factors that could contribute to nurses’ motivation and job satisfaction. These findings are consistent with the findings of other studies. Again, the study found that professional allowances paid to nurses, reduced rent, and the like are current motivation packages directed at nurses at the Korle-Bu teaching hospital. Conclusion: It is important to state that for motivation to accomplish its intended purpose, there is the need for the hospital to identify the individual needs to satisfy to enhance job satisfaction.
Identifying the Factors Affecting the Management of Addiction Treatment Clinics: A Qualitative Study
Authors: Abdi Iraj , Komeili Ali , Riahi Leila , Tabibi Seyed Jamaledin
Published: July 2021

Background: Due to the expansion of addiction treatment clinics and the costs that these clinics incur on the government and the families of addicts, monitoring the performance of these clinics and the need to pay attention to the principles of management, efficiency, and effectiveness is essential. Objectives: The present study was conducted to identify the factors affecting the management of addiction treatment clinics. Methods: The present study is a descriptive-applied and cross-sectional study conducted in 2019. Experts and academic experts have been considered as the research community, and 18 people were selected as the research sample by the purposeful snowball method. Data collection tools were the review of relevant national and international documents as well as semi-structured exploratory interviews. Finally, after collecting information from the interview sections and reviewing the sources, the data foundation and coding methods (open, axial, and selective) were used to classify the data. Results: The findings of the study showed that the effective factors in the management of addiction treatment clinics are dimensions such as organization, planning, control, guidance as well as leadership and treatment management. Conclusion: According to the findings of this study, it can be concluded that the effective factors in the management of addiction treatment clinics can be a good basis to evaluate managers so that the policies and programs of the organization can be upgraded, modified, and reviewed.
Thromboprophylaxis Measures to Prevent Venous Thromboembolism in Hospitalized Patients
Authors: Tolentino Júnior Dilceu Silveira , Carlos de Oliveira Roberto
Published: July 2021

Venous thromboembolism (VTE) is a serious complication in hospitalized patients and is the most frequent cause of preventable death in these patients, in addition to being the third cause of death of cardiovascular origin. Even though the loss of recent or continued mobility represents an important predisposition related to the onset of VTE, there are no clear and uniform criteria for defining the concept of immobility. Despite this, it is currently known that the early mobilization and other non-pharmacological thromboprophylactic measures of the patient in the medical or surgical clinic is an essential measure for his full recovery and satisfactory prognosis. Therefore, it is always important to prescribe preventive multiprofessional care, such as lateral decubitus variation, bed positioning, passive continuous movements apparatus as use of a cycle ergometer (CE), compressive socks, intermittent pneumatic compression (IPC), electric stimulation, sitting position, orthostatism, transfer from bed to chair, walking, and insertion of a filtering device in the lower portion of the main human vein in order to guarantee recovery quickly, safely and free from the risk of developing fatal complications such as pulmonary thromboembolism (PTE). Our objective in this article is to briefly summarize the main non-pharmacological thromboprophylaxis measures that help prevent venous embolism, in addition to addressing other mechanical resources used to prevent complications of thromboembolic events described in the literature on this topic.
Evaluation of Antibiotic Resistance Pattern of Escherichia coli Isolated From Broiler Chickens With Colibacillosis in Ardabil Province, Iran
Authors: Azizpour Aidin , Ghazaei Ciamak
Published: July 2021

Introduction: Colibacillosis is one of the most important bacterial diseases of birds that is caused by Escherichia coli. This disease causes considerable economic damage to the poultry industry every year. Various antimicrobial agents are used to reduce the damage caused by this infection. But in recent decades, the increased use of antibiotics has led to the development of resistant genes and, consequently increasing antibiotic resistance of bacteria, leading to a decrease in the efficacy of antibiotics. The purpose of this study was to determine the susceptibility and drug resistance of 178 isolates of 40 chicken flocks in Ardabil province northwest of Iran. Methods: Five carcasses were randomly selected from each flocks with colibacillosis and sampled from liver and heart using sterile swabs. After culture and isolation, colonies were identified by biochemical and serological methods. Antibiotic resistance of all isolates to 19 antibiotics was determined using disk diffusion method based on CLSI guidelines. Results: Of 200 samples, 178 (89%) were isolated, and 22 (11%) did not grow. In this study the highest antibiotic resistance was observed against flumequine (98.31%), nalidixic acid (97.25%), tylosin (97.20%), oxytetracycline (97.20%), chlortetracycline (95.50%), difloxacin (89.32%), doxycycline (81.47%), enrofloxacin (77.53%), sulfamethoxazole + trimethoprim (71.91%), and the lowest antibiotic resistance was recorded for Linco-Spectin (36.52%), chloramphenicol (22.47%), gentamycin (7.30%), fuzbac (5.05%) and ceftriaxone (3.93%). All isolates were highly sensitive to ceftazidime. Conclusion: The results of this study showed a high level of resistance to antibiotics commonly used in poultry industry, which is probably due to improper use of antibiotics in poultries.
The Study of Antibacterial Properties of Anbarnasara Smoke on Multi-Drug Resistant Bacteria Isolated From Urinary Infection in Pregnant Women
Authors: Molamirzaei Afsaneh , Allahdadian Maryam , Doudi Monir
Published: July 2021

Background: Using smoke from burning donkey dung has been popular in the treatment of many diseases in Iran. Objective: This study aimed to investigating the antimicrobial properties of donkey dung smoke on multi-drug resistant (MDR) bacteria isolated from urinary infection. Materials and Methods: First, 300 and 200 urine samples were collected from pregnant and non-pregnant women in Isfahan, Iran. Then in each group, 100 bacterial isolates including Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, and Staphylococcus saprophyticus were isolated. Antibiotic resistant protocol was determined by antibiogram test. Donkey dung was sterilized, disintegrated, and heated. The smokes were concentrated in n-hexane solvent (65%) and were collected after evaporation of the solvent. Finally, the antibacterial activities of the concentrations of 0.25, 0.5 and 1 mg/mL of the smokes were detected using disk diffusion and macrodilution methods. Results: The most abundant MDR isolates causing urinary infections in pregnant and non-pregnant women was Escherichia coli. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of donkey dung smoke on MDR isolates from pregnant women were 0.25 mg/mL and 0.5 mg/mL, respectively. In the case of MDR isolates in non-pregnant women, the MIC of the smoke on Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus was 0.25 mg/mL, and the MBC on these isolates was 0.5 mg/mL. Conclusion: The smokes from donkey dung investigated in the present study have suitable potentials for controlling the infections after In vivo analysis.
Anticancer Effect of Fractions From Staphylococcus aureus and Bacillus atrophaeus on the Proliferation and Death of Human Breast Cancer Cell Line (MCF-7)
Authors: Asadi Sepideh , Soleimani Neda
Published: July 2021

Background: Nowadays, breast cancer is known to be one of the most common cancers among women. Due to the side effects of chemotherapy and the high probability of recurrences in surgery, it is essential to identify and introduce new anticancer drugs of natural origin with fewer complications. In this regard, secondary bacterial metabolites and other microbial products have been considered. In the meantime, pathogenic and environmental bacteria have been investigated. Objective: The aim of this study is to examine the effects of the interaction between cytoplasmic extract and the cell wall of Staphylococcus aureus and Bacillus atrophaeus on the proliferation rate of human breast cancer cells. Materials and Methods: In this experimental study, cytoplasmic and cell wall extracts of bacteria were prepared. Then, SDS-PAGE was used to examine their protein contents. MCF-7 cells, as human breast cancer cells, with bacterial cytoplasmic extract and bacterial cell wall, were treated at different concentrations. Mesenchymal stem cells derived from adipose tissue were treated with different concentrations of bacterial cell wall extract. The effects of cytotoxicity were assessed by MTT assay at 24 and 48-hour intervals. The results were analyzed by SPSS. Results: The results showed that bacterial cytoplasmic extract had a concentration-dependent cytotoxic effect on cancer cells, suggesting that the increase of concentration significantly (P<0.05) increased cell death. Additionally, the bacterial cell wall extract showed a proliferative effect on cell growth (P<0.05) Conclusion: The bacterial cytoplasmic extract has a lethal effect and can, therefore, be considered as an anticancer compound in the future. This feature of the bacterium is attributed to the presence of a novel bioactive compound that can be used as an adjunct to other chemotherapy compounds. The bacterial cell wall extract, on the other hand, has cell growth-promoting components and can, therefore, be adopted as a compound for the proliferation of mesenchymal stem cells or wound healing in future studies.
Effect of Psidium guajava (guava) L. Leaf Decoction on Antibiotic-resistant Clinical Diarrhoeagenic Isolates of Shigella spp.
Authors: Daswani Poonam , Muthuraman Vigneshwaran , Macaden Ragini , Dias Mary , Birdi Tannaz
Published: July 2021

Background: Although shigellosis is self-limiting, antibiotics are recommended to minimize the severity of symptoms and reduce mortality rates. However, due to the increasing reports of antibiotic resistance, alternative approaches are needed to combat shigellosis. Interest for research on medicinal plants has increased in recent years, and hence, they can be explored to treat this infectious diarrhoea. Objective: To study the effect of Psidium guajava L. (guava) leaf decoction (GLD) on the antibiotic-resistant clinical isolates of Shigella spp. Materials and Methods: A total of 43 isolated Shigella spp. from diarrhoeal patients were used in this study. The effect of GLD on the bacterial viability was initially assessed. The isolates were divided into two categories: sensitive and resistant to GLD. For sensitive isolates, antibacterial activity of GLD was evaluated while for resistant strains, the ability of GLD for reducing the bacterial invasion of the HEp-2 cell line underwent an investigation. Results: Among the 43 Shigella isolates, GLD affected the growth of 23 strains. The invasion of 9 strains from the 20 remaining resistant isolates was unaffected. Although the number of isolates was less, the data suggested that isolates belonging to S. flexneri serogroup were more sensitive to GLD in comparison with other spp (i.e., sonnei, boydii, and dysenteriae). Conclusion: The results of this study revealed the efficacy of GLD against drug-resistant Shigella spp. and thus could be considered for the treatment of diarrhoea. GLD can be a cost-effective alternative to antibiotics.
Antibacterial Effect of Low-Level Laser (Diode 405 nm) on Antibiotic-Resistant Enterococci Clinical Isolates (In Vitro)
Authors: Khavari Rahimeh , Massudi Reza , Karmostaji Afsaneh , Soleimani Neda , Ashkeshi Pantea , Rezaei-Pandari Mohammad
Published: July 2021

Background: Enterococcus is a part of normal gastrointestinal flora in human body. Nevertheless, antibiotic-resistant Enterococcus (ARE) is considered a key factor in nosocomial infections which result in a considerable increase in the rate of patient death due to referring of numerous patients to health centers annually, or lead to extended disease convalescence. Objective: This study aimed to evaluate the bactericidal effect at 405nm diode at a laser power of 30 mW on ARE viability of clinical infections. Materials and Methods: In the present study, 30 isolates underwent antibiotic susceptibility test (AST) in which sensitivity to piperacillin (100 µg), rifampin (5 µg), and oxacillin (1 µg) were measured based on the Clinical and Laboratory Standards Institute (CLSI) guidelines. Afterwards, ten most resistant isolates were selected and irradiated by a 405 nm diode laser at a power of 30 mW for 180 and 240 seconds. The data were reported statistically as mean ± standard deviation, and the analysis of the data on varied bacteria was performed using ANOVA. The result was evaluated by SPSS software and P value ≤0.05 was interpreted to be significant. Results: Bacterial viability decreased unsteadily to 10 resistant isolates. Moreover, enhancing irradiation time caused a lower viability rate in such a way that the viability of isolate 9 having the lowest viability rate was reduced from 2.94% in 180 seconds to 0.58% in 240 seconds. The result was evaluated by SPSS software and P value was determined to be significant, and P≤0.05 was laser irradiation for either 180 s or 240 s. Conclusion: Following the study results, 405 nm diode laser could be applied as a tool for eliminating clinical ARE, and it was useful for preventing hospital-acquired infections.
Contamination of Water Sources of Karaj Hospitals with Legionella pneumophila and Campylobacter jejuni
Authors: Ghomimaghsad Niloofar , Yaslianifard Somayeh , Mohammadzadeh Mohammad , Dadashi Masoud , Noorisepehr Mohammad
Published: July 2021

Background: One of the most common routes of infection development in humans is contaminated water. Legionella pneumophila and Campylobacter jejuni are the important causes of community- and hospital-acquired pneumonia and gastroenteritis that are transmitted to humans via the inhalation of contaminated water droplets and consumption of contaminated water, respectively. Thus, continuous monitoring of the water supply systems for these pathogens has great importance in public health. Objective: This study aimed to evaluate the water contamination of Karaj hospitals with these two bacterial species. Materials and Methods: In this study, 62 water samples were obtained from different parts of the hospitals of Karaj from April to September 2019, including air conditioning systems, dialysis equipment, ventilation tanks, and different wards of a hospital such as infectious diseases, pediatrics, gastroenterology, dialysis, and intensive and neonatal intensive care units. The samples were collected in sterile containers and immediately transferred to the laboratory for further analysis. The culture on specific media, staining, and biochemical tests were performed to identify the L. pneumophila and C. jejuni. Results: Out of 62 water samples, 25.8% (16 samples) were positive for L. pneumophila; 68.75% were observed in hot water samples, and 31.25% were attributed to cold water samples. Among 62 samples, 4.84% (3 samples) were positive for C. jejuni, which were all detected in hot water samples. Conclusion: Considering that the methods of water refinery of municipal water have no high efficiency, the quality improvement of the water sources of hospitals seems to be necessary.
Out-of-Pocket Expenditures for Delivery for Maternity Waiting Home Users and Non-users in Rural Zambia
Authors: Fontanet Constance P. , Kaiser Jeanette L. , Fong Rachel M. , Ngoma Thandiwe , Lori Jody R. , Biemba Godfrey , Munro-Kramer Michelle , Sakala Isaac , McGlasson Kathleen Lucile , Vian Taryn , Hamer Davidson H. , Rockers Peter C. , Scott Nancy A.
Published: July 2021

Background: Utilizing maternity waiting homes (MWHs) is a strategy to improve access to skilled obstetric care in rural Zambia. However, out-of-pocket (OOP) expenses remain a barrier for many women. We assessed delivery-related expenditure for women who used MWHs and those who did not who delivered at a rural health facility. Methods: During the endline of an impact evaluation for an MWH intervention, household surveys (n = 826) were conducted with women who delivered a baby in the previous 13 months at a rural health facility and lived >10 km from a health facility in seven districts of rural Zambia. We captured the amount women reported spending on delivery. We compared OOP spending between women who used MWHs and those who did not. Amounts were converted from Zambian kwacha (ZMW) to US dollar (USD). Results: After controlling for confounders, there was no significant difference in delivery-related expenditure between women who used MWHs (US$40.01) and those who did not (US$36.66) (P=.06). Both groups reported baby clothes as the largest expenditure. MWH users reported spending slightly more on accommodation compared to those did not use MWHs, but this difference represents only a fraction of total costs associated with delivery. Conclusion: Findings suggest that for women coming from far away, utilizing MWHs while awaiting delivery is not costlier overall than for women who deliver at a health facility but do not utilize a MWH.
Survey on Zoonotic Importance and Prevalence of Dirofilaria immitis Infection in Dogs of Tabriz, Iran
Authors: Hashemzadeh Farhang Hossein , Bahavarnia Seyed Razi , Esmailzadeh Mohamad Javad , Mahmoudi Kamalabad Navid
Published: July 2021

Background: Dirofilaria immitis is one of the most important parasites of the circulatory system of dogs that causes severe cardiovascular events in the animal and it is reported to be the most common nematode in humans and dogs from many parts of Iran. Methods: In order to investigate the presence of Dirofilaria immitis infection in Tabriz, Iran, a cross-sectional study was performed on 200 owned and stray dogs. The blood samples were analyzed using Knott method. The prevalence of D. immitis infection was reported to be 15% in owned dogs. Results: The highest levels of infection were reported in dogs less than one year of age and the lowest in dogs aged over 9 years. In a statistical survey, there was a significant relationship between infection and gender. Conclusion: According to the findings of this study, due to the presence of D. immitis infection in this region, the need for a more comprehensive examination and control of the infection in this region is emphasized.
In Vivo Therapeutic Effect of Triclabendazole + Levamisole on Hydatid Cyst in Rats
Authors: Sadeghi Peyman , Khayatnouri Mirhadi , Hashemzade Farhang Hosein , Kaffash Elahi Ramin
Published: July 2021

Introduction: Hydatidosis is a global disease and one of the most dangerous zoonotic diseases which is found in areas where humans, dogs, and herbivores are in close contact with each other. Methods: In this study, a total of 64 rats were divided into control and case groups, and then 3000 protoscolices were injected into the peritoneal cavity. After 2 months, rats in the case group were given triclabendazole + levamisole at a dose of 6 mg + 4.41 mg/mL orally for 60 days, respectively. Then, after 6 months of infection, the rats of the control and case groups were killed by anesthesia and then an autopsy was performed and the viscera were carefully examined for hydatid cyst infection. Results: The results showed that in the control group, 3 cysts were observed in the liver, 10 in the kidney, and 88 in the lung. The mean number of hydatid cysts in this group was determined to be 33.6. In the case group, 2 cysts were observed in the liver, 6 in the kidney, and 64 in the lungs. The mean number of hydatid cysts in the viscera was 24 in the case group. Conclusions: Statistical analysis of the results obtained from the case and control groups showed that the therapeutic efficacy of triclabendazole + levamisole was 31%, which is not enough to treat cases of hydatid cyst.
A Case Report of Human Demodicosis in a Patient Referred to a Dermatology Clinic in Tabriz, Iran
Authors: Mohebbi Hesamoddin , Boozarjomehri Amniyeh Shayan , Mahdavi Parisa , Heydari Azar Heris Ali
Published: July 2021

Background: The genus Demodex belongs to the order Prostigmata and the family Demodecidae that has several species of uncommon mites, some of which cause severe scabies in animals. There are two species of this mite that cause disease in humans, including Demodex folliculorum, which is known as hair follicle mite, and Demodex brevis. This disease is more common in women than in men. Case Presentation: The patient is a 36-year-old woman living in one of the villages of Tabriz city who referred to a dermatologist following severe itching and hyperkeratosis (abundant dandruff) of the cheeks. Then, she was introduced to the laboratory for preparing a slide from a sample taken from the patient’s cheeks. A large number of Demodex mites were observed in the microscopic test of the sample. Conclusion: In patients referred to skin clinics with scaling and itching, especially in the head and face, the complication may be due to Demodex infection. Therefore, it is suggested that demodicosis be considered in differential diagnosis in such patients.
A Review of the Importance of Stool Test in the Diagnosis of Intestinal Parasites
Authors: Garedaghi Yagoob
Published: July 2021

Objectives: Stool test is a test used to diagnose an infection or intestinal parasitic infection. Gastrointestinal infections occur for a variety of reasons, so this test is valuable along with other complementary tests. Common gastrointestinal infections are commonly reported as food poisoning or stomach infections. However, diagnostic tests are useful for treating and removing the source of the disease. Methods: This study was reviewed by using a search of keywords including, "stool test" , "Diagnosis ", "Intestinal Parasites", in Google Scholar, PubMed, and Elsevier databases. Approximately 15 articles were selected that were examined thoroughly. Results: The results of our study revealed that the direct microscopic method is more widely used in medical diagnostic laboratories due to its time saving and simplicity of testing. Conclusion: Stool tests are recommended for people with symptoms of a gastrointestinal infection. These symptoms include: Prolonged diarrhea, abdominal pain (cramping), nausea and vomiting, Stools with blood and mucus. However, diagnostic tests are useful for treating and removing the source of the disease.
Evaluation of Rodent Fauna of Rural Cutaneous Leishmaniasis Reservoir in East Azerbaijan Province of Iran During 2018-2019
Authors: Heidarinejad Saeid , Esfandiyar Behrang , Amirfazli Mohsen
Published: July 2021

Introduction: Leishmaniasis is one of the most important parasitic diseases that represents a serious health problem worldwide. This disease exists in three forms: cutaneous (rural, urban), visceral, and mucocutaneous. In the rural type, some rodents play the role of a reservoir. In our country, many people are infected with this disease every year. Cutaneous Leishmaniasis is now common in some parts of East Azerbaijan province. Considering that no comprehensive study on the general condition of cutaneous Leishmaniasis reservoirs in this province has been done so far, this research was very necessary. This study was conducted to investigate the fauna, distribution, and frequency of rodents in East Azerbaijan province from 2018 to 2019. Methods: This is a descriptive cross-sectional study. Cluster sampling was performed on rodents. Rodents were caught each week by 30 live traps from different parts of East Azerbaijan province and were determined in the laboratory after examining their morphological characteristics. In order to identify Leishmaniasis, 2 samples were prepared from each rodent ear on a laboratory slide and after fixation with methanol and staining by Giemsa method, they were examined by light microscope. Results: A total of 100 rodents were found in this study, which included 5 species of Rhombomys opimus, Meriones libycus, Mus musculus, Nesokia indica, and Tatera indica. In this study, a total of 3 rodents infected with Leishmania parasite were caught, 2 of which were from Meriones libycus from Azarshahr and Sarab counties and 1 from R. opimus from Kalibar. Conclusion: Infection of R. opimus and M. libycus species was identified in Azarshahr, Sarab, and Kalibar counties. These two species are the most important reservoirs of cutaneous leishmaniasis in Iran. It is suggested that rodents be controlled in areas where there are cases of disease and rodents have been infected with Leishmania parasite in order to control the disease.
Vulvar Schistosomiasis: An Infrequent Localization of the Female Genital Schistosomiasis
Authors: Bourée Patrice , Belilovsky Clarence de , Plantier Françoise , Berville-Levy Sophie
Published: July 2021

Female genital schistosomiasis (FGS) is frequent in sub-Saharian Africa, Madagascar, and the Middle East, with localization in all organs of the reproductive tract, which causes many problems. However, vulvar localization of this disease is uncommon. The patient was a young woman of 25 years old with a complaint of a mass in the right labia minora. After the tumor removal, pathology examination revealed inflammatory granuloma with eggs of Schistosoma haematobium. The patient has never travelled to an endemic tropical area but has bathed in a contaminated river in Corsica, an island located in the south of France. The diagnosis of vulvar schistosomiasis can only be confirmed by biopsy of the lesion.
The Impact of COVID-19 Lockdown on Global Warming: A Call for Policy Review
Authors: Jegede Oluwatosin Samson , Olarewaju Sunday
Published: July 2021

Coronavirus disease 2019 (COVID-19) is probably the worst epidemic the world has experienced in recent times; it has led to drastic, lifesaving, and extraordinary decisions by governments of nations. Among such extraordinary measures is the closure of international borders leading to the cancellation of air travel by commercial airlines. The carbon emissions from air travel affect global warming. To this end, some authors ranked aircraft as if it were a country and compared the volume of carbon emissions generated by air travel to that generated by countries from other sources. Commercial air travel ranked seventh after Germany in terms of carbon emissions. This policy review, therefore, explored the impact of COVID-19 lockdown and travel restrictions on global warming. As a result of lockdown, there is a likelihood of a significant decrease in carbon emissions and global warming. Assuming the estimated global emissions remain constant annually, an estimated 9 gigatonnes of carbon dioxide emissions would be avoided by the end of 2020 provided that the lockdown continues. To accurately measure the value of reduced carbon dioxide emissions during the global lockdown, it is recommended that scientific studies be conducted to estimate the carbon emissions generated by the few aircraft granted waivers to transport essential commodities during the global lockdown and deduct it from the 9 gigatonnes. After the global lockdown, through a travel policy review, governments and organizations are encouraged to restrict physical meetings or activities that involve air travel only to situations where a physical presence is unavoidable.
Scientometric Evaluation of Published Articles in Travel Medicine and Global Health
Authors: Oh William Wen , Nasir Nizrull , Flaherty Gerard Thomas
Published: July 2021

Introduction: Travel medicine has become firmly established as a medical discipline and, as such, it should undergo periodic assessment of its productivity. Scientometrics is a field of study related to data science, which concerns itself with the measurement and analysis of published scholarly literature. Methods: A scientometric evaluation was conducted of all 240 articles published through February 2021 in the International Journal of Travel Medicine and Global Health (IJTMGH), one of the few academic journals dedicated to the study of travel medicine. Results: The majority of articles belonged to the general category of travel medicine (62%, n=149), with 38% of articles (n=91) being focused on global health and non-travel related infectious diseases. The 149 travel medicine-related articles mapped onto the Body of Knowledge syllabus of the International Society of Travel Medicine, with the majority of articles addressing the domain of pre-travel assessment of travellers (49.7%, n=74), including the sub-domains of patient evaluation, special populations of travellers, special itineraries, prevention and self-treatment, and communication of risks to travellers. The most common thematic designation of IJTMGH articles related to medical and health tourism (12.1%, n=29). High levels of geographic diversity, multi-authorship and inter-institutional collaboration were observed in the journal. The subject matter of the most cited and most popular articles reflected the broad coverage of travel medicine and global health by IJTMGH. Conclusion: We recommend that future bibliometric and citation analyses be performed, which will further enhance our understanding of the evolution of these dynamic fields of academic study.
The future potential of Annona muricata L. extract and its bioactive compounds as radiation sensitizing agent: proposed mechanisms based on a systematic review
Authors: Wijaya David Andi , Louisa Melva , Wibowo Heri , Taslim Aslim , Permata Tiara Bunga Mayang , Handoko Handoko , Nuryadi Endang , Kodrat Henry , Gondhowiardjo Soehartati Argadikoesoema
Published: July 2021

Despite technological advances in cancer treatment, especially in radiotherapy, many efforts are being made in improving cancer cell radio-sensitivity to increase therapeutic ratio and overcome cancer cell radio-resistance. In the present review, we evaluated the anticancer mechanism of Annona muricata L. (AM) leaves extract and its bioactive compounds such as annonaceous acetogenins, annomuricin, annonacin, or curcumin; and further correlated them with the potential of the mechanism to increase or to reduce cancer cells radio-sensitivity based on literature investigation. We see that AM has a promising future potential as a radio-sensitizer agent.
A Comparative Evaluation of Smear Layer Removal Using Erbium:YAG Laser-Activated Irrigation, Sonic Irrigation, and Manual Dynamic Irrigation: A Scanning Electron Microscope Study
Authors: Thapak Gourav , Arya Ashtha , Grewal Mandeep S. , Arora Anshul
Published: July 2021

Introduction: The conventional chemomechanical procedures are ineffective in complete disinfection of the pulp space due to the complexities of the root canal architecture. The present study aims to compare the efficacy of erbium: YAG laser-activated irrigation, sonic irrigation, and manual dynamic irrigation in the removal of the smear layer through a scanning electron microscope study. Methods: Fifty extracted single rooted mandibular premolars with single canal were used and instrumented until F3 ProTaper rotary file reached the working length. Upon the completion of the canal preparation, each specimen was irrigated with 3 mL of 4% NaOCl for 3 minutes, 3 mL saline for 1 minute and 3 mL of 17% EDTA for 3 minutes. The teeth were assigned to three experimental groups (n=15 each): manual dynamic irrigation, sonic irrigation (EndoActivator), and Er:YAG laser using an X pulse tip. Root canals were sectioned longitudinally and the smear layer at the apical, middle and coronal third was examined under a scanning electron microscope. Smear layer scores were analyzed by Kruskal-Wallis and Mann-Whitney U tests at P=0.05. Results: The Er:YAG laser group showed significantly lower smear layer scores in the apical third as compared to all other groups. EndoActivator resulted in better cleaning efficacy at the apical area compared to manual dynamic agitation. Conclusion: This study showed results in favor of Er:YAG with an X-pulse tip followed by EndoActivator activation.
The Successful Treatment of Eyelid Intradermal Melanocytic Nevi (Nevus of Miescher)With the Dual-Wavelengths Copper Vapor Laser
Authors: Ponomarev Igor Vladimirovich , Topchiy Sergey B. , Andrusenko Yury N. , Shakina Lyudmila D.
Published: July 2021

Introduction: Surgical methods for removing eyelid nevus are associated with a risk of developing such side effects as scarring or loss of eyelashes. Although current laser modalities have shown promising results, eyelid laser surgery may cause skin discoloration and noticeable scarring. This study aims at evaluating the efficacy and safety of the dual-wavelengths copper vapor laser (CVL) treatment of eyelid intradermal nevus of Miescher (NM). Patients and Methods: We present three adult female patients with eyelid intradermal nevus treated with CVL. CVL settings were as follows: the average power of 0.6-0.8 W, under a power ratio of 3:2 at wavelengths of 511 nm and 578 nm, exposure time of 0.3 seconds, the light spot diameter of 1 mm. Results: The dual-wavelength CVL treatment of medium- and large-sized NM provides the complete removal of eyelid dome-like NM after one and two sessions with a month interval correspondingly. The duration of skin healing accounted for two weeks. Conclusion: The complete removal of eyelid NM lesions without side effects demonstrates the safety and efficacy of dual-wavelengths CVL in treating such skin lesions. This laser treatment mode of benign skin neoplasms seems promising for dermatologists and cosmetologists’ clinical practice.
Dosage Effects of an 810 nm Diode Laser on the Proliferation and Growth Factor Expression of Human Gingival Fibroblasts
Authors: Karoussis Ioannis K. , Kyriakidou Kyriaki , Psarros Costas , Afouxenides Panayotis , Vrotsos Ioannis A.
Published: July 2021

Introduction: A substantial amount of evidence supports the positive effect of photobiomodulation on the proliferation and differentiation of various cell types. Several laser wavelengths have been used for wound healing improvement, and their actual outcome depends on the settings utilized during irradiation. However, the heterogeneous wavelengths and laser settings applied in the existing literature make it difficult to draw solid conclusions and comparison of different studies. The aim of the present study is to evaluate and compare the effects of various doses of laser energy, provided by an 810 nm diode, on human gingival fibroblasts in terms of proliferation and expression of growth factors with a pivotal role in wound healing. Methods: Human gingival fibroblasts were cultured on plastic tissue culture and irradiated with 2, 4, 6 or 12 J/cm2 . The effects of the low-level laser therapy (LLLT) using an 810 nm diode laser on growth factor expression (EGF, TGF and VEGF) were evaluated by qPCR at 72 hours and 7 days after irradiation. Cell proliferation was evaluated at 24, 48 and 72 hours after LLLT using MTT assay. Results: Energy density of 12 J/cm2 provoked irradiated gingival fibroblasts to demonstrate significantly higher proliferation as well as higher gene expression of Col1, VEGF and EGF. LLLT positive effects were obvious up to 7 days post-irradiation. Conclusion: LLLT with 810 nm presents beneficial effects on proliferation, collagen production and growth factor expression in human gingival fibroblast cells. The application of 12 J/cm2 can be suggested as the optimal energy density for the enhancement of the wound healing process.
Effect of a Low-Level Laser on Liposomal Doxorubicin Efficacy in a Melanoma Cell Line
Authors: Razzaghi Mohammad Reza , Ghazimoradi Mohammad Hossein , Afzali Shervin , Kamani Ehsan , Mohajerani Ezeddin , Shirkavand Afshan , Farivar Shirin
Published: July 2021

Introduction: The cytotoxicity of chemotherapy drugs is a significant challenge in the way of surmounting cancer. Liposomal drug delivery has proven to be efficacious in increasing the function of the drugs. Its potential to accumulate drugs in the target site and enhance the efficiency of anti-cancer agents with lower doses hinders their cytotoxicity on normal healthy cells. Since the release of drugs from liposomes is not generally on a controlled basis, several studies have suggested that external stimuli including lasers could be used to induce controlled release and boost the efficiency of liposomal drug delivery systems (LDDSs). Methods: The A375 cancer cell line was used and exposed to the liposomes containing doxorubicin in the presence of a low-level laser beam to investigate its effect on the liposomal stimuli-responsiveness release and its toxicity on cancer cells. So as to achieve that goal, Annexin V/PI was employed to analyze the number of cells that underwent apoptosis and necrosis. Results: Here, we report the effect of laser irradiation on LDDSs. According to the results obtained from the annexin V/PI assay, the pattern of viability status has shifted, so that the number of pre-apoptotic cells treated with liposomal doxorubicin and a laser beam was more than that of cells treated with only liposomal doxorubicin. Conclusion: The use of stimuli-responsive LDDSs, in this case, laser-responsive, has led to favorable circumstances in the treatment of cancer, offering enhanced cancer cell cytotoxicity.
In vitro and in vivo studies of cytotoxic effects of FeSO4 nanoparticles
Authors: Moeini Elahe , Bonyadian Mojtaba , Ebrahimnejad Hadi , Karimi Iraj , Askari Nahid
Published: July 2021

Background and aims: Using iron as a food additive usually causes undesirable sensory changes and side effects in humans. In this study, we made iron (Fe) nanoparticles (NPs) and studied the cytotoxicity of FeSO4 bulk and NPs on HT-29 cells and different doses of these particles on rat intestine. Methods: Particle size of nanoscale was achieved by mechanical technique. Iron particles were characterized using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The effect of iron particles with different concentrations (6.25, 3.125, and 1.57 mM/mL) on the colon cell line was performed using the MTT assay at 24, 48, and 72 hours. Apoptosis and necrosis of the cells were assessed using Annexin V-FITC staining and propidium iodide (PI) at 24 h. In an in vivo study, Taftoon bread was produced from fortified wheat flour with FeSO4 bulk and NPs, which are recommended in human diet (9, 18, and 27 mg of elemental iron/kg flour). Wistar rats were fed daily with fortified bread for 21 days and their colon and small intestine were then evaluated histopathologically. Statistical analyses were performed using SPSS 22.0 software by chi-square test. Results: The synthesized FeSO4 NPs were smaller than 100 nm, and they had more adverse effects on the viability of the HT-29 cells compared to the bulk- FeSO4 at 72 hours. Flow cytometric study showed that the early apoptosis of cells by the bulk form was more than the NPs, but at the low concentration (1.57 mM/mL), the NPs induced more necrosis than the bulk particles (P=0.063). The survival rate of cells facing all concentrations of NPs and bulk- FeSO4 decreased dose dependently (P=0.075). In vivo results revealed that there were no pathological changes in rats’ intestinal tissues. Conclusion: The bulk and NPs of iron have adverse effects on the HT-29 cells, but no histopathological changes were seen on rats’ intestinal cells.
Antiproliferative effects of fresh water crab hemolymph and meat extract on breast cancer cell line
Authors: Rezakhani Leila , Alizadeh Morteza , Sharifi Esmaeel , Soleimannejad Mostafa , Alizadeh Akram
Published: July 2021

Background and aims: Despite the advances in drugs, side effects of chemotherapy drugs continue to exist. Therefore, more attention has been paid to the compounds derived from medicinal herbs and aquatic organisms. This study aimed to investigate the effect of freshwater crab hemolymph and meat extract on breast cancer (BC) cell line (4T1). Methods: After isolation of freshwater crab hemolymph and meat extract, protein concentration and total antioxidant capacity were analyzed by bicinchoninic acid (BCA) and cupric reducing antioxidant capacity (CUPRAC) methods. The 4T1 cells and bone marrow mesenchymal stem cells (BMSCs) were treated with crab hemolymph (1, 2, 10 mg/mL) and meat extract (0.1, 0.2 and 1 mg/mL), and cell survival was analyzed using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT) at 48 and 72 hours. Nitric oxide (NO) secretion was measured by Griess method. Data were analyzed using one-way analysis of variance (ANOVA). Results: Protein concentration of 23.25 mg/mL was shown in crab hemolymph, and 2.3 mg/mL in meat extract. Total antioxidant capacity was reported as 1.036 µM/mL and 1.104 µM/mL in crab hemolymph and meat extract, respectively. Cell survival in the 4T1 cells was decreased in a dose- and time-dependent manner (P≤0.001). NO secretion of 4T1 cells was decreased after treatment with different concentrations of crab hemolymph and meat extract at 48 and 72 hours. Cellular growth was observed in BMSCs after treatment with different concentrations of crab hemolymph and meat extract at 48 and 72 hours. Conclusion: Since crab hemolymph and meat extract have protein and antioxidant activities, they can have anti-cancer effects on 4T1 cells.
Optimization of human interferon beta protein expression in Chinese hamster ovary cells
Authors: Xodadadi Nasrin , Saeidinia Alireza , Zeinoddini Mehdi , Khalilzadeh Rasoul
Published: July 2021

Background and aims: Human interferon beta-1a (hIFNβ-1a) is a 22.5-kDa glycoprotein used to treat diseases such as multiple sclerosis (MS). Because of appropriate post-translation modifications, protein isolation, and lack of toxicity in Chinese hamster ovary (CHO) cells, we cloned hIFNβ-1a encoding sequence into these cells by recombinant DNA technology to achieve stable expression of this recombinant protein. Methods: The hIFNβ-1a encoding sequence was designed based on the CHO cells’ codon usage and the Gene Bank data, and then syntactically constructed in the pUC57 vector. After confirmation, the synthesized sequence was cloned into the pcDNA3.1 expression vector by using EcoRI and XhoI sites via Escherichia coli DH5α competent cells. Then, the recombinant vector pcDNA-hHIFNβ1a was linearized by BglII and transfected into the CHO cells using lipofectamine. The transfected cells were proliferated and screened by gentamicin. Certain concentrations of zinc sulfate, DMSO, and glycerol were used to enhance protein expression. Finally, the recombinant protein expression was qualitatively evaluated using different techniques. Results: The hIFNβ1a integrity was confirmed by DNA sequencing and specific software. The construction and sub-cloning of hIFNβ1a-pcDNA3.1 in E. coli were confirmed by colony-PCR with specific primers and restriction enzyme mapping. The screening of transfected CHO cells was performed using gentamicin. The protein expression was confirmed by RT-PCR, MTT assay, SDS-PAGE, and Western blot. Comparison of the optimized and control samples demonstrated that chemical treatment enhanced the protein expression. Conclusion: We achieved the stable clones of CHO cells expressing the active form of human interferon beta.