bluuebunny/crossref_metadata_2025 huggingface dataset:
Selection as the best method of genetic improvement in high temperature germinated bell pepper cultivars
Authors: Oliveira Ariane Miranda de , Lima Maida Cynthia Duca de , Silva Tânia Santos , Amaral Claudio Lucio Fernandes , Louzada Rômullo Oliveira , Santos Rayka Kristian Alves , Oliveira Elismar Pereira de
Published: April 2022

This work was carried out to estimate genetic parameters for the germination of seeds of four varieties of bell pepper to assist in choosing the most suitable genetic material for regions with high temperatures. The study covered two experiments, each comprising a germination temperature of 35.0 °C and an ambient temperature with an average of 19.6 °C, for the cultivars All big, Amarelo Alegria, Casca dura Ikeda and Yolo wonder. The design used was randomized, with eight replicates composed of 25 seeds each, totaling four treatments with 200 seeds per experiment. The germination percentage of the seeds was evaluated at temperatures of 19.6 °C and 35.0 °C, later used to obtain the genetic parameters. Bell pepper cultivars showed significant genetic variation to the detriment of the environmental one, showing that the genetic factor contributed more than the environmental factor to the studied correlation. Despite the low percentage of germination observed at 35.0 °C, the highest values were obtained for the cultivars All big, and Yolo wonder. The predominance of genetic and phenotypic influence on seed germination to the detriment of environmental impact, even in high temperatures, allows the continuity of bell pepper production in thermal conditions above 30.0 °C, mainly for the cultivars All big and Yolo wonder.
Síndrome de sobreposição de Lúpus Eritematoso Sistêmico e Líquen Plano Oral: relato de caso
Authors: Lira Rafaela Martins , Oliveira Catarina Rodrigues Rosa de , Kotovicz Leticia Bandeira de Melo , Omena Andréa Magalhães Agra de , Abreu Eulina Maria Vieira de , Ferreira Sonia Maria Soares
Published: April 2022

A Síndrome de Sobreposição (SS) associada ao Lúpus Eritematoso Sistêmico (LES) e Líquen Plano (LP), entidade rara, é caracterizada por sinais clínicos e imuno-histopatológicos sincrônicos de ambos. Relatamos o caso de uma paciente do sexo feminino, 39 anos, com diagnóstico de LES, apresentando lesões intraorais cujas hipóteses diagnósticas foram Líquen Plano Oral (LPO) e LES. Neste cenário, o presente estudo teve como objetivo descrever as patologias citadas, assim como expor as dificuldades do processo diagnóstico da síndrome de sobreposição em questão, explanando as etapas da doença e o tratamento realizado. O trabalho foi realizado através de um estudo de caso com objetivos descritivos e abordagem qualitativa utilizando-se de conhecimentos clínicos da dermatologia, estomatologia e reumatologia, com base em pesquisa bibliográfica e revisão de literatura integrativa em bases de dados digitais juntamente com a análise de exames complementares, histopatológicos e de imunofluoerescência. Na biópsia de lesões clinicamente ambíguas, como no caso das lesões orais da paciente, características histopatológicas de um ou ambos os processos podem ser encontrados simultaneamente ou oscilando em períodos diferentes, dificultando o diagnóstico e complicando o prognóstico e tratamento. Assim, foi observado que a imunofluorescência direta (IFD) tornou-se uma ferramenta essencial para auxiliar no diagnóstico dessa condição. Este caso reforça que a SS possui um diagnóstico desafiador e seu tratamento envolve vários profissionais para gerenciá-la.
Estratégia nutricional para o emagrecimento pós-parto
Authors: Souza Anne Karoline Mafra de , Ferreira José Carlos de Sales
Published: April 2022

Introdução: O período após o parto, é conhecido por puerpério, no pós-parto por sua vez, faz com que o corpo volte as condições normais, e costuma ser variável por conta da amamentação, este período bloqueia a ovulação. Acredita-se que no pós-parto, todos os órgãos, exceto as mamas, já estão em condições previas, que independem da amamentação. Objetivo: apresentar estratégias nutricionais para emagrecimento pós-parto. Metodologia: trata-se de uma revisão de literatura com abordagem descritiva, com artigos publicados em plataformas digitais nos anos de 2011 a 2022, com os descritores Obesidade, pós-parto, obesidade no pós-parto, estratégias nutricionais. Resultados e Discussão: A obesidade é uma inflamação corporal, denominada como uma doença crônica, adquirida por meio de hábitos alimentares, inadequados, as mulheres gestantes tendem a ganhar peso, devido a diversos fatores da gestação, e para perdê-los após a gestação custa cerca de 4 a 6 semanas, e é necessário se alimentar de proteínas de alto valor biológico, e alimentos com baixo índice glicêmico. Conclusão: É necessário que no período puerpério a mulher viva o resguardo, pois durante esse período os órgãos voltam ao seu tamanho normal. Sobre alimentação, é necessário equilibrar em quantidades e qualidade, para o balanço energético seja positivo.
Desafios do atendimento odontológico às pessoas com deficiência auditiva em uma clínica escola do Norte do Brasil
Authors: Feitosa Karine da Cruz da Silva , Santana Evilen Cristina dos Santos , Silveira Débora Furtado da , Hidalgo Lídia Regina da Costa
Published: April 2022

A deficiência auditiva é considerada uma das deficiências mais prevalentes e, no contexto do atendimento odontológico, a dificuldade em atender ao paciente com deficiência auditiva de forma integral constitui um problema de saúde relevante, porém, pouco abordado. Nesse sentido, o presente estudo objetivou avaliar o conhecimento, a segurança e as dificuldades dos graduandos em Odontologia, frente ao atendimento de pacientes com deficiência auditiva, que utilizam a Língua Brasileira de Sinais (LIBRAS).  Assim, foi realizado um estudo observacional transversal e descritivo com aplicação de questionários a 107 acadêmicos do curso de Odontologia, divididos em Períodos Iniciais (1º ao 5º) e Períodos Finais (6º ao 10º). Dos entrevistados, 97% declararam possuir conhecimento quanto ao que é LIBRAS, sendo que 98% consideram que ela ajuda na formação profissional. No entanto, 95% dos acadêmicos não se sentem preparados para atender um deficiente auditivo. Os graduandos (76%) responderam que LIBRAS deveria ser matéria obrigatória na grade curricular. Não houve diferença estatística entre as respostas dos acadêmicos dos períodos iniciais e finais. Assim pode-se inferir que LIBRAS deve estar presente na grade curricular do curso de Odontologia, para melhorar a interação entre paciente e profissional, extinguindo possíveis dificuldades durante o atendimento.
Mandibular wisdom tooth impaction and angulation in relation to the mandibular ramus among yemeni students: prevalence and pattern
Authors: Abulohom Faisal , Al-Sharani Hesham Mohammed , Alhasani Abdalhaq Hussin , Al-Muaalemi Zakarya , Al-Hutbany Nassr Abdalwhab , Aldomaini Mohammed Sadiq , Al-Radhi Mohammed Abdulwahab , Hu Tenglong
Published: April 2022

Background: The goal of this cross-sectional study was to determine the mandibular third molar impaction in an adult Yemeni student using the Pell & Gregory and Winter classification. Methods: An orthopantomographic x-ray was used to assess 200 students (mean age 22.34 years), 102 males and 98 females. The data was subjected to statistical tests, which included age, gender, angulation, width, and impaction depth. Results: Of 345 mandibular third molars examined, mandibular third molars were congenitally missing in about 14% of the cases, approximately 7.5% in females and 6.5 % in males. According to Pell and Gregory's classification, this study found a significant bilateral relation, with type A being the most prevalent in roughly half of the participants (61.4%). Type C, on the other hand, came in second with 22.9%, and this relationship was significant on both sides, particularly in women, who had greater mandibular third molar impaction than men. The vertical angulations of mandibular third molars showed the greatest vertical angulation (82.02%), followed by mesioangular (16.5%). Conclusion: The impaction pattern of mandibular third molars in Yemeni culture revealed a significant incidence of mesioangular, level B, and class II impaction, with more females than males.
Mapping of Patents Related to The Development of Products for Diabetic Neuropathy: a technological prospection from 2010 to 2022
Authors: Barros Giselda dos Santos , Pereira Hilton Henrique Cruz Santos , Souza Maria Suely Regis , Camargo Maria Emília , Menezes Paula dos Passos , Silva Gabriel Francisco da
Published: April 2022

Diabetic neuropathy is a complication of diabetes that occurs in 40-60 million diabetics worldwide and is associated with other chronic diseases. Besides that, there are no prospective studies that present the state of the technique about technologies developed to circumvent this important public health problem. Given the above, this study aims to carry out a technological prospection about the products developed for diabetic neuropathy in the period 2010-02/02/2022. To this end, a technological prospection was carried out at the INPI and ESPACENET patent office’s always making a parallel between Brazilian reality in comparison with international companies. Therefore, in this period, it was found that the countries that produced the most patents on the subject were China, the United States, and Japan, and the company that published the most patents in this period was Beijing Luyuan Qiuzheng Technology Dev. Co Ltd. Thus, the data from this study showed that Brazil did not produce patents on the subject in this period and that China, the United States, and Japan were the countries that showed the greatest interest in technological development aimed at this disease, which affects millions of people all over the world.
A influência percebida da indústria 4.0 no ensino de enfermagem: revisão integrativa
Authors: Soares Tânia Catarina Sobral , Carmo Annibal José Roris Rodriguez Scavarda do
Published: April 2022

Introdução: A existência da globalização não seria possível sem a indústria 4.0. A mesma tem forte contribuição para economia, meio ambiente, educação e saúde. Objetivo: desse estudo foi realizar uma revisão integrativa nas produções científicas que envolvessem os diversos domínios da indústria 4.0 no Ensino em Enfermagem. Métodos: trata-se de uma revisão integrativa, nas bases de dados eletrônicas Biblioteca Virtual em Saúde (BVS); Periódicos CAPES e Pubmed. O acesso as bases de dados se deram de janeiro a fevereiro de 2022. Resultados: Após rigor aplicado, foram selecionados 18 artigos, depois ler 30 artigos na íntegra. Foram descartados 12, cujo assunto não pertenciam ao ensino em enfermagem. Os artigos selecionados estão distribuídos do ano de 2015 a 2021, com diversos tipos de estudos, sendo a maioria descritivo qualitativo com coleta de dados realizada através de entrega de formulário eletrônico ou presencialmente com entrevistas. Tais estudos apontaram a necessidade de aprofundamento na indústria 4.0 representadas no ensino em enfermagem. Conclusão: Os resultados encontrados nesse estudo responderam à pergunta de pesquisa e expuseram a influência percebida da indústria 4.0 para o ensino em enfermagem. Esse estudo deixa como recomendação a exploração de cada ramificação da indústria 4.0, sendo elas: impressão 3D, internet das coisas, aplicativos, redes sociais, gamificação, big date e podcast, cuja importância é analisar efetividade e eficácia no ensino em enfermagem.
Intervenção em crianças com Transtorno do Espectro Autista aliada a um protocolo de desenvolvimento
Authors: Castro Gisélia  Gonçalves de , Santos Francielle  Marfisa de Paula , Santos Nilce Maria de Freitas , Ramos Salvador Boccaletti , Figueiredo Gloria Lúcia Alves
Published: April 2022

Este estudo teve como objetivo observar os efeitos de uma intervenção em crianças diagnosticadas com Transtorno do Espectro Autista (TEA) por meio da Escala de Desenvolvimento Motor. Trata-se de um estudo transversal, exploratório com abordagem quantitativa. Participaram do estudo 28 crianças com diagnóstico clínico de TEA de ambos os sexos e seus pais ou responsáveis. Para a coleta de dados foi utilizada a Escala de Desenvolvimento Motor. A amostra foi dividida nos seguintes grupos: Grupo Intervenção (G1) e Grupo Controle (G2) separadas em análises Pré Intervenção, Pré Controle, Pós-intervenção e Pós Controle. Os dados foram analisados pelo programa estatístico JASP (versão 0.11.1.). As comparações entre as médias dos grupos foram feitas por meio de análise de variância com medidas repetidas e as análises post-hoc por meio do Teste de Bonferroni. A homogeneidade dos dados foi verificada por meio do Teste de Levene. O tamanho do efeito para as comparações duas-a-duas do Teste de Bonferroni foi estimado por meio da estatística "d" de Cohen. Verificou-se também que tanto no domínio Idade Motora Geral quanto no Quociente Motor Geral houve significância no grupo onde se comparou o Pré-intervenção com o Pós-intervenção denotando uma influência positiva dos procedimentos intervencionistas utilizados (p≤0,05). Conclui-se que o método de intervenção utilizado foi benéfico para as crianças.
Consulta de enfermagem puerperal na atenção primária: evidências científicas
Authors: Córdova Gabriella Dalla Corte , Fiorenza Lorena , Colomé Juliana Silveira , Vasconcelos Janine , Santos Naiana Oliveira dos
Published: April 2022

Objetivo: identificar as evidências científicas nacionais e internacionais acerca das ferramentas disponíveis para consulta de enfermagem puerperal na atenção primária. Métodos: trata-se de uma revisão integrativa da literatura, cujas buscas foram realizadas nas seguintes bases de dados: BDENF; LILACS; MEDLINE e SCIELO. Foram incluídos 13 artigos, a análise foi realizada por meio da matriz avaliativa que considera a caracterização do estudo, resposta à pergunta de pesquisa e nível de evidência. Resultados: após a pré-análise, exploração do material e interpretação originaram-se duas categorias que permitiram sintetizar os achados: Ferramentas utilizadas para mediar a consulta de enfermagem no puerpério e Estratégias tecnológicas da enfermagem para a educação em saúde no pós-parto. Conclusão: os achados do presente estudo apontam diversas ferramentas e estratégias tecnológicas utilizadas para executar a consulta de enfermagem no período do ciclo gravídico-puerperal. Acredita-se que ao fomentar o uso, a criação e o aprimoramento dessas atividades seja uma forma de otimizar, qualificar e padronizar o cuidado.
Equipes de saúde frente aos cuidados a crianças em tratamento oncológico: principais intervenções
Authors: Souza Lizandra Ellem Silva de , Farias Anderson Fernandes de Carvalho , Koproski Ana Claudia , Sousa Cássio Moura de , Santos Dulcicleide Rodrigues , Oliveira Renata Silva , Lira Damião Wellington de Sousa , Silva Wesley Francisco Sousa da , Apolinário Joelma Maria dos Santos da Silva , Silva João Felipe Tinto
Published: April 2022

O objetivo do presente estudo, é identificar os principais cuidados, e as intervenções realizadas no tratamento oncológico para crianças com o diagnóstico de câncer. Trata-se de um levantamento bibliográfico sendo então uma revisão de literatura. Realizada no período de janeiro a abril de 2022 através de uma busca nas bases de dados biblioteca Virtual em Saúde (BVS), Scientific Electronic Library Online (SciELO) e Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS). Os descritores da saúde utilizados foram: equipe de saúde AND tratamento oncológico AND pediatria. Os critérios de inclusão foram: aqueles pulicados em qualquer idioma desde que tenha tradução disponível, artigos publicados no período de 2018 a 2022 disponíveis de forma gratuita, e nas bases de dados citadas anteriormente. Diante dos achados foi possível identificar 450 artigos que após aplicar os critérios de elegibilidade e feita a leituras dos restantes totalizou 12 trabalhos utilizados. O diagnóstico de câncer é de difícil aceitação e traz consigo sentimentos de desesperança. É uma doença agressiva e que exige um tratamento específico que necessita de intervenções pelas equipes de saúde. Alguns cuidados que podem ser realizados são: cuidados paliativos, comunicação, oferta de um ambiente confortável para o paciente, e suas famílias, apoio psicológico entre outros.  Conclui-se que as equipes de saúde estão diretamente ligadas ao cuidado a crianças em tratamento oncológico, pois tem o conhecimento, e capacidades técnicas para prestar esse serviço.
Dewey's theory and its contributions for Science Teaching
Authors: Siqueira Vanessa Fagundes , Goi Mara Elisângela Jappe
Published: April 2022

Through this work, we sought to present aspects related to John Dewey's life and theory, highlighting elements referring to scientific problems arising from common sense; the individual's experience and contributions to cognitive development; the importance of the development of democracy in the school context and the teaching by investigation defended by the school conceived by Dewey. Through this study, the importance that relationships and experiences in a cultural environment acquire for the improvement of an individual's knowledge is identified. Accordingly, teaching for Dewey suggests being mediated through problem solving, involving situations that are part of the students' reality, bringing school concepts closer to their daily lives and helping them to create the habit of seeking solutions to the issues that worry them. in and out of school.
O cuidado de enfermagem na prevenção de hematomas no setor de hemodinâmica
Authors: Francisco William Mannerick , Flauzino VIctor Hugo de Paula , Peruchena Giseli da Silva Moura , Cesário Jonas Magno dos Santos
Published: April 2022

Uma equipe de enfermagem treinada corretamente e continuamente é indispensável para se ter o sucesso terapêutico e recuperação dos pacientes que foram submetidos à Intervenção Percutânea (IP). O enfermeiro deve conhecer os procedimentos, benefícios, riscos e complicações, para nortear seu cuidado baseado em ações de enfermagem que busca garantir uma assistência de qualidade, evitando eventos adversos relacionados às IP. O objetivo Geral do estudo é descrever as principais causas de hematomas nos pacientes após o procedimento em hemodinâmica. Descrever as complicações de um hematoma em pacientes que foram submetidos aos procedimentos hemodinâmicos, as possíveis causas de hematomas, analisar os cuidados de enfermagem que foram realizados para a prevenção antes do procedimento cirúrgicos e identificar os cuidados de enfermagem para os pacientes que desenvolveram hematomas. Metodologia: revisão bibliográfica que reuniu evidências para responder a pergunta de pesquisa: qual é o índice de complicações relacionadas à hematomas após realizar intervenções percutâneas nos procedimentos hemodinâmicos?. Os serviços de Hemodinâmica são setores de alta complexidade que necessitam de profissionais capacitados e treinados. A equipe de enfermagem tem papel fundamental na prevenção de complicações após a intervenção percutânea, a posição em que o paciente deve permanecer “decúbito dorsal”, o tempo para deambular após a retirada da bainha introdutora e o uso de dispositivos de oclusão arterial o mais utilizado “curativo compressivo, esses três cuidados são fundamentais na prevenção de sangramentos e hematomas.
Correção de assimetria dentogengival através da técnica minimamente invasiva Flapless - Estudo de caso clínico
Authors: Couto Aline Vieira , Marzagão Mila , Vilela Júnior Rafael de Aguiar
Published: April 2022

A cirurgia plástica periodontal tem sido recomendada para pacientes que apresentam insatisfação com seu sorriso ou desproporção em relação a sua anatomia gengival, dessa forma sua corretada identificação é crucial para planejar a conduta mais apropriada de tratamento e propiciar maior previsibilidade de sucesso. Sendo assim, o presente trabalho tem como objetivo demonstrar por meio de um relato de caso clínico a correção do sorriso gengival através de uma técnica minimamente invasiva denominada Flapless. Paciente, caucasiana, sexo feminino, 31 anos de idade, apresenta como queixa principal insatisfação         com o tamanho e o aparecimento de seus dentes ao sorrir. Após avaliação clínica optou-se por realizar a cirurgia para aumento de coroa clínica utilizando a técnica flapless. Após 45 dias do procedimento observou-se cicatrização completa dos tecidos, e a partir do exame de profundidade de sondagem pode-se confirmar a efetividade da conquista do espaço biológico. Diante do caso supracitado observou-se que a correção da assimetria dentogengival pela técnica sem retalho (flapless) é um procedimento seguro, fácil e previsível, que possui diversos pontos positivos, dentre eles: menor sangramento e tempo cirúrgico, queda da inflamação no pós-operatório, não necessidade de sutura e mínimo desconforto durante a recuperação. Sua viabilidade e aplicabilidade clinica é notória, com chances reduzidas de recidivas e alto índice de sucesso final.
Knowledge, attitude, and practice of surgical site infection prevention among operating room nurses in southwest China
Authors: Feng Wen , Sae-Sia Wipa , Kitrungrote Luppana
Published: April 2022

Background: Surgical site infection has become a problem in the operating room, and the nurses’ knowledge, attitude, and practice could impact the incidence of the infection. Unfortunately, there is a dearth of studies on this issue in China. Therefore, determining nurses’ knowledge, attitude, and practice of surgical site infection prevention is necessary.
Objectives: This study aimed to examine the knowledge, attitude, and practice of surgical site infection prevention and their relationships, as well as to identify differences in knowledge, attitude, and practice of surgical site infection prevention according to nurses’ demographic characteristics.
Methods: A sample of 999 operating room nurses participated in 49 tertiary hospitals and 75 secondary hospitals in Guizhou Province, Southwest China. Data were collected using validated questionnaires through a Chinese survey website. Data were analyzed using descriptive statistics, One-Way Analysis of Variance, and Pearson product-moment correlation.
Results: The knowledge of surgical site infection prevention was at a low level, the attitude was positive, and the practice was at a high level. Approximately 39% of the nurses passed knowledge scores of ≥ 70%, 60% gave a positive attitude score of ≥ 80%, and 76% achieved a practice score of ≥ 80%. The nurses’ attitude was positively related to knowledge (p < .01), and practice (p < .01), respectively. However, knowledge and practice did not significantly relate. The age group of 30-39 years old had significant higher knowledge than other age groups. The nurses with working experience of 6-15 years had significant higher knowledge scores than other groups. In addition, the nurses with one time of training frequency had significant lower attitude and practice scores than those with six to ten times of training frequency.
Conclusion: Approximately 60% of operating room nurses still had inadequate knowledge regarding surgical site infection prevention, but they had a positive attitude and high level of practice. The findings of this study might serve as an input for nurse administrators or policymakers to provide updated knowledge or guideline, closed supervision, and in-service training on surgical site infection prevention for operating room nurses.
Comparative Report on the National Policies and Political-Economic Context of Germany, Poland, Sweden, and the UK
Authors: Crossdale Rachel , Foster Liam , Walker Alan
Published: April 2022

Context
The demographic context for the EIWO project is continued population ageing and, especially, growth in the numbers of very elderly people, which applies to all four countries in this project: Germany, Poland, Sweden and the UK. However, the common ageing trend is unequally distributed. In particular there remain significant gaps in life expectancy (LE) and health life expectancy (HLE) both between countries and within them. For example LE differences based on socio-economic status, income, and education are present in all four countries. In terms of HLE, Swedish women have HLE at birth, that is, on average, 6 years longer than German women, 8 years longer than Polish women and 11 years longer than British women.
RELIGIOSITY OF THE MALAY BATUBARA TROUGH ANTHROPOLOGICAL AND HISTORICAL APPROACH
Authors: Maulana Cecep , Loebis Iin Almeina
Published: April 2022

This research is talked about the Islamic Religious Discourse of Malay Batu Bara. In this case, the researchers took the historic sites in Batu Bara as a support in the Islamic religion of Malay Batu Bara. Background with many historic sites Batu Bara which is considered by some people to have high noble values and touched to religious part of the community and make researcher interested to explore this anthropology history approach which found the contribution about various aspects of human life, society and culture but also showed  the procedure, technique and process. That is systematically arranged in disciplinary research to develop knowledge and obtain the object study. To implement this research the writer uses the methodology with a qualitative approach which results in descriptive data and the object under study is the Islamic religious of Malay Batu Bara. In collecting data researchers use the power of primary and secondary data. In analyzing the data researchers use data reduction, data presentation, and concluding that do data verification. Then in the validity of the data the researcher uses the degree of trustworthiness, dependability, and certainty. In the scientific , researchers use anthropology  history. Researchers found many historic sites in Batu Bara which are very close. They are Kubah Datok Batu Bara, Sumur Istana Niat Lima Laras and Meriam Bogak. From the three historic sites, the researcher found a theoretical component from religion as said by C.Y.Glock and R. Stark that is the dimension of faith, dimension ritualistic, dimension experiential, dimension consequential, and dimension intellectual. Besides that researchers also found religious Malay Batu Bara community also influenced by beliefs in occult things such as witchcraft, rituals rejecting reinforcements, sea spells, and also related to beliefs in traditions or customs such as abstinence forbids, tapai party and balimau bathing.
Sustainable Management of Medicago sativa for Future Climates: Insect Pests, Endophytes and Multitrophic Interactions in a Complex Environment
Authors: McNeill Mark R. , Tu Xiongbing , Altermann Eric , Beilei Wu , Shi Shengjing
Published: April 2022

Medicago sativa L. (alfalfa, syn. lucerne) is an important forage crop for livestock, which is subject to attack from a range of insect pests and susceptible to diseases that can reduce production and persistence. This review considers the main insect pests affecting M. sativa in China and New Zealand as well as the wider plant resistance mechanisms and multitrophic interaction that occur between plants, insect pests, entomopathogens, endophytes, the environment, and climate change. This is with a view to identifying new research opportunities applicable to M. sativa that can be applied to improving production and persistence of this important agricultural crop. These opportunities include identification and activity of entomopathogens/endophytes (e.g., Bacillus and Pseudomonas spp., Metarhizium spp.) and plant growth enhancers (Trichoderma), as well as multitrophic plant-insect-microbial interactions.
A Template Model Explains Jerboa Gait Transitions Across a Broad Range of Speeds
Authors: Ding Jiayu , Moore Talia Y. , Gan Zhenyu
Published: April 2022

For cursorial animals that maintain high speeds for extended durations of locomotion, transitions between footfall patterns (gaits) predictably occur at distinct speed ranges. How do transitions among gaits occur for non-cursorial animals? Jerboas (Jaculus) are bipedal hopping rodents that frequently transition between gaits throughout their entire speed range. It has been hypothesized that these non-cursorial bipedal gait transitions are likely to enhance their maneuverability and predator evasion ability. However, it is difficult to use the underlying dynamics of these locomotion patterns to predict gait transitions due to the large number of degrees of freedom expressed by the animals. To this end, we used empirical jerboa kinematics and dynamics to develop a unified spring Loaded Inverted Pendulum model with defined passive swing leg motions. To find periodic solutions of this model, we formulated the gait search as a boundary value problem and described an asymmetrical running gait exhibited by the jerboas that emerged from the numerical search. To understand how jerboas change from one gait to another, we employed an optimization approach and used the proposed model to reproduce observed patterns of jerboa gait transitions. We then ran a detailed numerical study of the structure of gait patterns using a continuation approach in which transitions are represented by bifurcations. We found two primary mechanisms to increase the range of speeds at which gait transitions can occur. Coupled changes in the neutral leg swing angle alter leg dynamics. This mechanism generates changes in gait features (e.g., touchdown leg angle and timings of gait events) that have previously been shown to induce gait transitions. This mechanism slightly alters the speeds at which existing gait transitions occur. The model can also uncouple the left and right neutral leg swing angle, which generates asymmetries between left and right leg dynamics. New gait transitions emerge from uncoupled models across a broad range of speeds. In both the experimental observations and in the model, the majority of the gait transitions involve the skipping and asymmetrical running gaits generated by the uncoupled neutral leg swing angle mechanism. This simulated jerboa model is capable of systematically reproducing all biologically relevant gait transitions at a broad range of speeds.
Cell Inertia: Predicting Cell Distributions in Lung Vasculature to Optimize Re-endothelialization
Authors: Chan Jason K.D. , Chadwick Eric A. , Taniguchi Daisuke , Ahmadipour Mohammadali , Suzuki Takaya , Romero David , Amon Cristina , Waddell Thomas K. , Karoubi Golnaz , Bazylak Aimy
Published: April 2022

We created a transient computational fluid dynamics model featuring a particle deposition probability function that incorporates inertia to quantify the transport and deposition of cells in mouse lung vasculature for the re-endothelialization of the acellular organ. Our novel inertial algorithm demonstrated a 73% reduction in cell seeding efficiency error compared to two established particle deposition algorithms when validated with experiments based on common clinical practices. We enhanced the uniformity of cell distributions in the lung vasculature by increasing the injection flow rate from 3.81 ml/min to 9.40 ml/min. As a result, the cell seeding efficiency increased in both the numerical and experimental results by 42 and 66%, respectively.
Differences Between Supplier and Customer Experiences of Marketing Mix in the Construction Industry
Authors: Al-Fadly Ahmad
Published: April 2022

Suppliers often contend they “know” best when it comes to what the customer wants. Yet, despite using advanced models, such as the 7P (product, place, price, promotion, people, process, and physical evidence) marketing mix, companies can fail to meet their service objectives because customers perceive services from their own perspective. This difference in perspective presents a knowledge gap that requires it to be filled in an objective way. This research study elucidates how differences between supplier perception and customer perception of delivery are objectively identified. In this study, the target population consisted of the managers of the construction companies in Kuwait and their customers as individual property owners of residential, commercial, and industrial buildings. Discrete questionnaires consisting of the 7P marketing mix constructs were created specifically for the supplier and customer. Using a five-point Likert scale, data from 210 supplier staff and 210 customers were collected from the construction industry. The structural equation modeling (SEM) established the beta coefficients of latent variables reflecting the perceptions of both the supplier and customer. Both models were tested for internal consistency reliability using Cronbach’s alpha, and convergent validity was established based on the standardized factor loading and average variance extracted (AVE). In addition, discriminant validity was established using AVE and correlations. The differences between the standardized coefficients of supplier and customer coefficients were then tested against their pooled variance. The results show that suppliers tend to have a higher perception of their delivery on some constructs, while customers maintained a higher perspective on other constructs, that is, suppliers perceived that their product, place, price, and process were adequate, while customer data indicated otherwise. However, it was promising that the constructs of promotion, physical evidence, and people were more favorable among customer perceptions than supplier expectations. Interestingly, customers perceived that the people factor behind the construction industry was excellent. The findings recommend that suppliers scale their perceptions to be closer to the reality perceived by the customers. The study concludes that this approach of evaluating supplier–customer perceptions is highly beneficial to the supplier.
Long Non-Coding RNAs as Molecular Biomarkers in Cholangiocarcinoma
Authors: Wu Yanhua , Hayat Khizar , Hu Yufei , Yang Jianfeng
Published: April 2022

Cholangiocarcinoma (CCA) is a biliary system cancer that has the characteristics of strong invasiveness, poor prognosis, and few therapy choices. Furthermore, the absence of precise biomarkers for early identification and prognosis makes it hard to intervene in the early phase of initial diagnosis or recurring cholangiocarcinoma following surgery. Encouragingly, previous studies found that long non-coding RNA (lncRNA), a subgroup of RNA that is more than 200 nucleotides long, can affect cell proliferation, migration, apoptosis, and even drug resistance by altering numerous signaling pathways, thus reaching pro-cancer or anti-cancer outcomes. This review will take a retrospective view of the recent investigations on the work of lncRNAs in cholangiocarcinoma progression and the potential of lncRNAs serving as promising clinical biomarkers and therapeutic targets for CCA.
Stem Cells, Hematopoiesis and Lineage Tracing: Transplantation-Centric Views and Beyond
Authors: Konturek-Ciesla Anna , Bryder David
Published: April 2022

An appropriate production of mature blood cells, or hematopoiesis, is essential for organismal health and homeostasis. In this developmental cascade, hematopoietic stem cells (HSCs) differentiate into intermediate progenitor types, that subsequently give rise to the many distinct blood cell lineages. Here, we describe tools and methods that permit for temporal and native clonal-level HSC lineage tracing in the mouse, and that can now be combined with emerging single-cell molecular analyses. We integrate new insights derived from such experimental paradigms with past knowledge, which has predominantly been derived from transplantation-based approaches. Finally, we outline current knowledge and novel strategies derived from studies aimed to trace human HSC-derived hematopoiesis.
Effects of the Urease Concentration and Calcium Source on Enzyme-Induced Carbonate Precipitation for Lead Remediation
Authors: Wang Lin , Cheng Wen-Chieh , Xue Zhong-Fei , Hu Wenle
Published: April 2022

Heavy metal contamination during the rapid urbanization process in recent decades has notably impacted our fragile environments and threatens human health. However, traditional remediation approaches are considered time-consuming and costly, and the effect sometimes does not meet the requirements expected. The present study conducted test tube experiments to reproduce enzyme-induced carbonate precipitation applied to lead remediation under the effects of urease concentration and a calcium source. Furthermore, the speciation and sequence of the carbonate precipitation were simulated using the Visual MINTEQ software package. The results indicated that higher urease concentrations can assure the availability of CO32− during the enzyme-induced carbonate precipitation (EICP) process toward benefiting carbonate precipitation. The calcium source determines the speciation of carbonate precipitation and subsequently the Pb remediation efficiency. The use of CaO results in the dissolution of Pb(OH)2 and, therefore, discharges Pb ions, causing some difficulty in forming the multi-layer structure of carbonate precipitation and degrading Pb remediation. The findings of this study are useful in widening the horizon of applications of the enzyme-induced carbonate precipitation technology to heavy metal remediation.
An Antibiotic-Loaded Hydrogel Demonstrates Efficacy as Prophylaxis and Treatment in a Large Animal Model of Orthopaedic Device-Related Infection
Authors: Boot Willemijn , Foster Andrew Lewis , Guillaume Olivier , Eglin David , Schmid Tanja , D’Este Matteo , Zeiter Stephan , Richards Robert Geoff , Moriarty Thomas Fintan
Published: April 2022

Local antibiotic therapy is increasingly being recognised for its role in preventing and treating orthopaedic device-related infection (ODRI). A bioresorbable, injectable gentamicin-loaded hydrogel has been developed to deliver local antibiotics at the time of surgery with potential for both prevention and treatment of ODRI. In a prophylaxis model, the antibiotic hydrogel was compared with systemic perioperative antibiotic prophylaxis alone in twelve sheep (six per group) at the time of intramedullary (IM) nail insertion to the tibia, which was inoculated with methicillin-sensitive Staphylococcus aureus (MSSA). In a treatment model of single-stage revision surgery, adjunctive antibiotic-loaded hydrogel was compared with systemic antibiotics alone in a single stage revision of MSSA infection associated with a tibia intramedullary nail in eleven sheep (five/six per group). The primary endpoint was quantitative microbiological results of soft tissue, bone and sonicate fluid from explanted hardware at the time of euthanasia. At euthanasia, the control sheep that received no local antibiotics in the prophylaxis model were all culture-positive (median 1x108, range 7x106-3x108 colony forming units, CFU) while only two of six sheep receiving local gentamicin had any culture positive biopsies (median 1x101, range 0 - 1x105 CFU). For the treatment model, sheep receiving only systemic antibiotics were all culture-positive (median 8x105, range 2x103- 9x106 CFU) while only two of six sheep treated with gentamicin-loaded hydrogel had any culture positive biopsies (median 3x102, range 0 - 7x104 CFU). Local gentamicin concentrations measured in extracellular fluid in the tibial canal show a burst release of gentamicin from the hydrogel. Serum gentamicin concentrations peaked in both models at one day post application and were below detection limit thereafter. This study has demonstrated the effective use of a locally delivered antibiotic hydrogel for both the prevention and treatment of ODRI that is superior to that of systemic antibiotics alone. Future studies will endeavour to translate from preclinical to clinical research trials.
TRAF3 Positively Regulates Host Innate Immune Resistance to Influenza A Virus Infection
Authors: Chen Fangzhao , Chen Liurong , Li Yinyan , Sang Huiting , Zhang Chunyu , Yuan Shuofeng , Yang Jie
Published: April 2022

Tumor necrosis factor receptor-associated factor 3 (TRAF3) is one of the intracellular adaptor proteins for the innate immune response, which is involved in signaling regulation in various cellular processes, including the immune responses defending against invading pathogens. However, the defense mechanism of TRAF3 against influenza virus infection remains elusive. In this study, we found that TRAF3 could positively regulate innate antiviral response. Overexpression of TRAF3 significantly enhanced virus-induced IRF3 activation, IFN-β production, and antiviral response, while TRAF3 knockdown promoted influenza A virus replication. Moreover, we clarified that inhibiting ubiquitinated degradation of TRAF3 was associated with anti-influenza effect, thereby facilitating antiviral immunity upon influenza A virus infection. We further demonstrated the key domains of TRAF3 involved in anti-influenza effect. Taken together, these results suggested that TRAF3 performs a vital role in host defense against influenza A virus infection by the type-I IFN signaling pathway. Our findings provide insights into the development of drugs to prevent TRAF3 degradation, which could be a novel therapeutic approach for treatment of influenza A virus infection.
ECG Utilization Patterns of Patients With Arrhythmias During COVID-19 Epidemic and Post-SARS-CoV-2 Eras in Shanghai, China
Authors: Li Cheng , Chen Mu , Li Mohan , Wang Haicheng , Qiu Xiangjun , Hu Xiaoliang , Wang Qunshan , Sun Jian , Yang Mei , Zhu Yuling , Liao Peng , Zhou Baohong , Chen Min , Liu Xia , Zhao Yuelin , Shen Mingzhen , Huang Jinkang , Luo Li , Wu Hong , Li Yi-Gang
Published: April 2022

Background

The COVID-19 pandemic has led to concerns around its subsequent impact on global health.

Objective

To investigate the health-seeking behavior, reflected by ECG utilization patterns, of patients with non-COVID-19 diseases during and after COVID-19 epidemic.

Methods

Taking advantage of the remote ECG system covering 278 medical institutions throughout Shanghai, the numbers of medical visits with ECG examinations during the lockdown (between January 23 and April 7, 2020), post-lockdown (between April 8 and December 31, 2020) and post-SARS-CoV-2 (between January 23 and April 7, 2021) periods were analyzed and compared against those during the same periods of the preceding years (2018 and 2019).

Results

Compared with the same period during pre-COVID years, the number of medical visits decreased during the lockdown (a 38% reduction), followed by a rebound post-lockdown (a 17% increase) and a fall to the baseline level in post-SARS-CoV-2 period. The number of new COVID-19 cases announced on a given day significantly correlated negatively with the numbers of medical visits during the following 7 days. Medical visit dynamics differed for various arrhythmias. Whereas medical visits for sinus bradycardia exhibited a typical decrease-rebound-fallback pattern, medical visits for atrial fibrillation did not fall during the lockdown but did exhibit a subsequent increase during the post-lockdown period. By comparison, the volume for ventricular tachycardia remained constant throughout this entire period.

Conclusion

The ECG utilization patterns of patients with arrhythmias exhibited a decrease-rebound-fallback pattern following the COVID-19 lockdowns. Medical visits for diseases with more severe symptoms were less influenced by the lockdowns, showing a resilient demand for healthcare.
Relationship Between Hypertension and Basilar Atherosclerosis in Chinese Han Population: A High-Resolution Magnetic Resonance Imaging Study
Authors: Hu Feng , Lu Feng , Xiao Huiling , Dong Meixue , Xu Yan
Published: April 2022

Objective

To investigate the relationship between hypertension and basilar atherosclerosis evaluated by high-resolution magnetic resonance imaging (HR-MRI) in the Chinese Han population.

Methods

High resolution-MRI vessel wall imaging was performed in selected 193 patients for various indications. Multivariable logistic regression models based on odds ratio (OR) with their associated 95% confidence interval (CI) were used to assess the relationship between hypertension and basilar artery (BA) plaque, moderate or severe stenosis of BA plaque, and vulnerable plaque. A linear regression model was used to assess the relationship between hypertension and BA plaque numbers.

Results

Patients with hypertension had a higher proportion of BA plaque and vulnerable plaque as well as more number of enhancements of BA plaque and serious plaque compared with normotensive patients (all values of p < 0.05). Multivariable logistic regression analysis indicated that patients with hypertension had an increased risk for and more number of enhancements of BA plaque (adjusted-OR: 4.32, 95% CI 1.89–9.88, p < 0.001; adjusted-β: 0.55, 95% CI 0.14–0.96, p = 0.009, respectively) and had a higher proportion of moderate or severe stenosis of BA plaque and vulnerable plaque (adjusted-OR: 3.08, 95% CI 0.77–12.32, p = 0.111; adjusted-OR: 4.52, 95% CI 1.50–13.64, p = 0.007, respectively) compared with the normotensive group. Moreover, there was a saturation effect of age on the prevalence of BA plaque and vulnerable plaque.

Conclusion

Hypertension was the independent risk factor of BA plaque and vulnerable plaque assessed by HR-MRI in the Chinese Han population.
Novel Cardiac Computed Tomography Methods for the Assessment of Anthracycline Induced Cardiotoxicity
Authors: Feher Attila , Baldassarre Lauren A. , Sinusas Albert J.
Published: April 2022

Anthracyclines are among the most frequently utilized anti-cancer therapies; however, their use is frequently associated with off-target cardiotoxic effects. Cardiac computed tomography (CCT) is a validated and rapidly evolving technology for the evaluation of cardiac structures, coronary anatomy and plaque, cardiac function and preprocedural planning. However, with emerging new techniques, CCT is rapidly evolving to offer information beyond the evaluation of cardiac structure and epicardial coronary arteries to provide details on myocardial deformation, extracellular volume, and coronary vasoreactivity. The potential for molecular imaging in CCT is also growing. In the current manuscript we review these emerging computed tomography techniques and their potential role in the evaluation of anthracycline-induced cardiotoxicity.
Contagion Dynamics for Manifold Learning
Authors: Mahler Barbara I.
Published: April 2022

Contagion maps exploit activation times in threshold contagions to assign vectors in high-dimensional Euclidean space to the nodes of a network. A point cloud that is the image of a contagion map reflects both the structure underlying the network and the spreading behavior of the contagion on it. Intuitively, such a point cloud exhibits features of the network's underlying structure if the contagion spreads along that structure, an observation which suggests contagion maps as a viable manifold-learning technique. We test contagion maps and variants thereof as a manifold-learning tool on a number of different synthetic and real-world data sets, and we compare their performance to that of Isomap, one of the most well-known manifold-learning algorithms. We find that, under certain conditions, contagion maps are able to reliably detect underlying manifold structure in noisy data, while Isomap fails due to noise-induced error. This consolidates contagion maps as a technique for manifold learning. We also demonstrate that processing distance estimates between data points before performing methods to determine geometry, topology and dimensionality of a data set leads to clearer results for both Isomap and contagion maps.