bluuebunny/crossref_metadata_2025 huggingface dataset:
Opposite and Differently Altered Postmortem Changes in H3 and H3K9me3 Patterns in the Rat Frontal Cortex and Hippocampus
Authors: Dulka Karolina , Lajkó Noémi , Nacsa Kálmán , Gulya Karoly
Published: March 2024

Temporal and spatial epigenetic modifications in the brain occur during ontogenetic development, pathophysiological disorders, and aging. When epigenetic marks, such as histone methylations, in brain autopsies or biopsy samples are studied, it is critical to understand their postmortem/surgical stability. For this study, the frontal cortex and hippocampus of adult rats were removed immediately (controls) or after a postmortem delay of 15, 30, 60, 90, 120, or 150 min. The patterns of unmodified H3 and its trimethylated form H3K9me3 were analyzed in frozen samples for Western blot analysis and in formalin-fixed tissues embedded in paraffin for confocal microscopy. We found that both the unmodified H3 and H3K9me3 showed time-dependent but opposite changes and were altered differently in the frontal cortex and hippocampus with respect to postmortem delay. In the frontal cortex, the H3K9me3 marks increased approximately 450% with a slow parallel 20% decrease in the unmodified H3 histones after 150 min. In the hippocampus, the change was opposite, since H3K9me3 marks decreased steadily by approximately 65% after 150 min with a concomitant rapid increase of 20–25% in H3 histones at the same time. Confocal microscopy located H3K9me3 marks in the heterochromatic regions of the nuclei of all major cell types in the control brains: oligodendrocytes, astrocytes, neurons, and microglia. Therefore, epigenetic marks could be affected differently by postmortem delay in different parts of the brain.
Aurantiochytrium sp. Meal as Feed Additive for Pacific White Shrimp Reared under Low Temperature and Challenged by WSSV in Association with Thermal Stress
Authors: Hoffling Flávia Banderó , Marquezi Alex Silva , Pinheiro Isabela , Simon Cedric , Rombenso Artur Nishioka , Seiffert Walter Quadros , Vieira Felipe do Nascimento , Schleder Delano Dias
Published: March 2024

A study was conducted to test the inclusion of Aurantiochytrium sp. meal in the rearing of Penaeus vannamei grown in a clear water system and at a suboptimal temperature of 22 °C. The doses tested were 0 (control), 1, 2, 3, and 4% at a stocking density of 100 shrimp/m3. Rearing was carried out with aeration and individual heaters, and seawater temperature was controlled with a chiller. After nine weeks, shrimp were weighed and hemolymph was collected for hemato-immunological tests, and growth performance were calculated. Shrimp raised at 22 °C and fed a 1% of dietary supplementation of Aurantiochytrium sp. meal were superior in immunological parameters. After the dietary assay, a total of 42 shrimps (10.9 ± 0.06 g) per treatment were infected with White Spot Syndrome Virus (WSSV). They were orally infected with 2.6 ± 106 virus particles per g of the animal, maintained at a suboptimal temperature of 22 °C for 108 h, and acclimated to an optimal temperature of 28 °C for an additional 48 h. At 7 days post infection, surviving shrimp were collected for hemato-immunological analysis. Cumulative mortality results showed that shrimp fed diets containing 3% and 4% Aurantiochytrium sp. meal had higher survival than other treatments when challenged with WSSV.
Hedonic Analysis of Dried Pasta Prices Using E-Commerce Data—An Explorative Study
Authors: Bimbo Francesco , De Meo Emilio , Carlucci Domenico
Published: March 2024

Italy is the world leader in the production of pasta and the Italian market is characterized by strong price competition among large industrial producers. Thus, recently, many small and medium firms have started to differentiate their products as a way to achieve higher margins and escape from price competition. Using data on the prices and characteristics of dried pasta sold online in the Italian market and a hedonic price model, we estimated the implicit prices associated with several attributes that are currently available for dried pasta. We find that the “artisanal” statement on pasta labeling is associated with the highest price premium. Also, results show that protected geographical indication, Halal and Kosher certifications, and the use of ancient wheat varieties are valuable features of dried pasta sold in the Italian market. Instead, a positive, albeit limited in magnitude, price premium is associated with dried pasta made using 100% Italian durum wheat semolina, the organic method, enriched with additional ingredients. Findings suggest that producers can differentiate their products by mostly emphasizing their small-scale production methods, the territorial connotation, and the cultural and environmental sustainability of production. Otherwise, certifying dried pasta as Halal or Kosher can represent a complementary or alternative strategy to differentiate the product and achieve a higher price.
Effectiveness of Cyclic Voltammetry in Evaluation of the Synergistic Effect of Phenolic and Amino Acids Compounds on Antioxidant Activity: Optimization of Electrochemical Parameters
Authors: Jara-Palacios María José , Begines Emilio , Heredia Francisco J. , Escudero-Gilete María Luisa , Hernanz Dolores
Published: March 2024

Antioxidant activity can be evaluated using cyclic voltammetry (CV). The aim of this work is to verify the efficacy of CV in evaluating the synergistic effect of bioactive compounds, such as phenolic and amino acid compounds, on antioxidant activity. Therefore, three types of model solutions were prepared: individual model solution (phenol and amino acid), (b) binary model solutions (phenol-phenol and amino acid-amino acid) and (c) mixed phenol–amino acid solutions. Electrochemical measurement conditions were optimized for phenolic compounds (pH 3.0, 1.0 g/L and 100 mV/s) and for amino acids (pH 7.0, 2.0 g/L for amino acids and 100 mV/s), and, for each solution, the functional groups responsible of the anodic and cathodic peaks were established. The peak anodic potential (Epa) and the onset potential (Eon) were two parameters of great importance. The first one was used to classify the solutions according to their antioxidant potential. In general, all the binary and mixed solutions had lower values of Epa than the corresponding individual model solution, which indicates an improvement in the antioxidant potential. The second one was used to evaluate the synergistic effects of phenolic compounds and amino acids.
Milk Bioactive Compounds and Gut Microbiota Modulation: The Role of Whey Proteins and Milk Oligosaccharides
Authors: Gallo Valentina , Arienzo Alyexandra , Tomassetti Federica , Antonini Giovanni
Published: March 2024

A strong correlation between the occurrence of various pathological conditions and intestinal dysbiosis is supported by a range of strong evidence. Vice versa, many pathologies have been shown, in turn, to be responsible for alterations in the gut microbiota, a condition that can worsen illness outcomes and response to therapies. For these reasons, great efforts have been made, and studies are still ongoing, to elucidate the mechanisms underlying gut microbiota alterations and to search for pharmacologic or other strategies that can effectively restore the gut microbiota. In this narrative review, we examined the most significant literature on the role of some milk bioactive compounds, such as milk oligosaccharides and whey proteins, in modulating the composition of the gut microbiota and the underlying mechanisms of action, with the aim of investigating the impact of the microbiota changes mediated by these milk bioactive molecules on human health, and their potential use as therapeutics to treat or adjuvate the treatment of gut dysbiosis and associated pathologies.
Adulteration of Brain Health (Cognitive, Mood, and Sleep Enhancement) Food Supplements by the Addition of Pharmaceutical Drugs: A Comprehensive Review of Analytical Approaches and Trends
Authors: Paiva Rafael , Correia Manuela , Delerue-Matos Cristina , Amaral Joana S.
Published: March 2024

In recent years, the consumption of dietary supplements has grown worldwide, particularly in developed regions. However, this growing market has also become a prime target for adulteration practices, with some manufacturers illegally adding pharmaceuticals into plant-based food supplements (PFS) to enhance their effects. While extensive research has focused on detecting adulterant drugs in PFS tailored for improving sexual performance, weight loss, and muscle building, less attention has been given to supplements intended for mood enhancement, sleep aid, and cognitive function (nootropics). Nonetheless, recent reports indicate an increasing level of adulteration within this group of PFS. Therefore, this review aims at providing a comprehensive overview on the adulteration of PFS tailored for brain health, with a focus on the analytical techniques utilized for detection while also presenting data on consumption patterns and the prevalence of reported adulterants. Considering that the detection of such fraudulent practices primarily relies on chromatographic techniques coupled with mass spectrometry (MS), the developments in this field comprising either targeted or untargeted analysis of pharmaceutical adulterants are discussed.
A Metabolomics Approach to Establish the Relationship between the Techno-Functional Properties and Metabolome of Indian Goat Yoghurt
Authors: Rehman Hameedur , Saipriya Kanchanpally , Singh Ashish Kumar , Singh Richa , Meena Ganga Sahay , Khetra Yogesh , Sharma Heena
Published: March 2024

Introduction: Goat milk has poorer fermentation characteristics due to the absence or only traces of αs1-casein, due to which goat yoghurt contains a less dense gel structure. Moreover, the fermentation characteristics of the milk vary between the breeds of the same species. Therefore, it becomes imperative to explore a few metabolites which could regulate the techno-functional properties of goat yoghurt. Objectives: This study was aimed at relating the metabolite profile of yoghurt prepared from milk of Barbari, an indigenous goat breed of India, and its techno-functional properties (firmness, whey syneresis, and flow behaviour) using multivariate data analysis and regression models. Results: Goat yoghurt was prepared with two different total solids (TS) levels (12 and 16%) and cultures, namely, commercial culture comprising a thermophilic yoghurt culture (A) and NCDC-263 comprising a mixed yoghurt culture (B). Results demonstrated a significant difference (p < 0.05) in whey syneresis with the increase in the TS level. Flow behaviour of all yoghurt samples showed a decrease in viscosity with an increase in shear rate, which confirmed its non-Newtonian behaviour and shear thinning nature, whereas frequency sweep confirmed its viscoelastic nature. Firmness was the most affected under the influence of different TS and culture levels. It was higher (p < 0.05) for 16-A, followed by 16-3B, and minimum for 12-2B. GC-MS-based metabolomics of the yoghurt revealed a total of 102 metabolites, out of which 15 metabolites were differentially expressed (p < 0.05), including 2-hydroxyethyl palmitate, alpha-mannobiose, and myo-inositol. Multivariate data analysis revealed clear separation among groups using principal component analysis and several correlations using a correlation heat map. Further, regression analysis exhibited methylamine (0.669) and myo-inositol (0.947) with higher regression coefficients (R2 values) exceeding 0.6, thus demonstrating their significant influence on the techno-functional properties, mainly firmness, of the yogurt. Conclusion: In conclusion, A gas chromatography-based metabolomics approach could successfully establish a relationship between the metabolome and the techno-functional properties of the yoghurt.
Differential Characteristics of the Metabolic Profiles and Microbial Community between Superior and Normal Grades of Nongxiangxing-daqu
Authors: Hou Xiaoge , Hui Ming , Gu Xiaoman , Shi Xin , Fan Chenming , Wang Junfei , Li Xuesi , Pan Chunmei , Li Ruifang
Published: March 2024

Nongxiangxing-daqu (NXDQ), as a saccharification and fermentation agent, directly affects the flavor and yield of fresh Nongxiangxing Baijiu (NXBJ). The difference in fermentation temperature owing to the artificial turning operation leads to the formation of superior (S) and normal (N) grades of NXDQ. Here, aiming to explore the discriminant characteristics of two grades of NXDQ, we studied the physicochemical properties, volatile compounds and microbial communities using HS-SPME-GC/MS and high-throughput sequencing technology. The NXDQ grades presented different physicochemical properties. Staphylococcus, Weissella, Lactobacillus and Thermoascus were dominant in the S grade (S-NXDQ), while Bacillus, Thermoactinomyces and Aspergillus were predominant in the N grade (N-NXDQ). Higher alcohols, aldehydes and ketones positively correlated with the bacterial biomarkers could be used as metabolic biomarkers for N-NXDQ; the S-NXDQ had a higher abundance of key enzymes involved in lactic acid and ethanol fermentation, while N-NXDQ had a higher abundance of key enzymes involved in amino acid synthesis and long-chain fatty acid and lipid metabolism. N-NXDQ and S-NXDQ had different microbial and metabolic biomarkers. These findings provide insight into the discriminant characteristics of different grades of NXDQ, a theoretical basis for rational evaluation of NXDQ, and effective information for quality improvement of daqu.
Applications of Life Cycle Assessment in the Chocolate Industry: A State-of-the-Art Analysis Based on Systematic Review
Authors: Wang Shuhan , Dong Yahong
Published: March 2024

Chocolate is a popular food for its unique flavor and taste, rich nutritional value, and the psychological values brought to people. The raw material production of chocolate, product manufacturing, sales and transportation have different degrees of environmental impact. This review explores the environmental hot spots in the life cycle of chocolate and puts forward corresponding suggestions for the improvement. By applying a systematic review method, this paper collected 25 articles on life cycle assessment (LCA) of the environmental impact of the chocolate industry. It is found that the life cycle of chocolate has the highest environmental impact in the raw material production and chocolate manufacturing stages (accounting for 77–97% of total impacts), among which milk powder, sugar and cocoa derivatives are the important contributors to significant environmental burden. Dark chocolate generates the lowest carbon emissions (1.67 kg CO2 eq/kg product) among existing chocolate categories, while the chocolate confectionery products release the highest carbon emissions (6.76 kg CO2 eq/kg product) among chocolate-containing products. Improvement measures are proposed for reducing environmental impacts and for selecting environmentally friendly product formulae. This study can provide benchmarking for the chocolate industry and improves the understanding of life cycle environmental impacts of chocolate products.
Cryoprotective Effects and Quality Maintenance of Antifreeze Proteins and Peptides on Aquatic Products: A Review
Authors: Fan Xinru , Geng Wenhao , Li Meng , Wu Zixuan , Ma Yongsheng , Li Zhibo , Benjakul Soottawat , Zhao Qiancheng
Published: March 2024

Aquatic products are gaining popularity due to their delicacy and high nutrient value. However, they are perishable, with a short shelf-life. Frozen storage is associated with adverse effects, leading to protein oxidation and degradation, thereby altering the protein’s structural integrity and subsequently influencing the palatability of protein-based food products. To address these challenges, novel antifreeze peptides have gained significant attention. Antifreeze peptides are a class of small molecular weight proteins or protein hydrolysates that offer protection to organisms in frozen or sub-frozen environments. They offer distinct advantages over conventional commercial antifreeze agents and natural antifreeze proteins. This review provides an overview of the current state of research on antifreeze agents, elucidates their characteristics and mechanisms, and examines their applications in aquatic products. Furthermore, the article offers insights into the prospective development and application prospects of antifreeze peptides.
Comparison of the Adsorption and Desorption Dynamics of Biological Molecules on Alginate Hydrogel Microcapsules—The Case of Sugars, Polyphenols, and Proteins
Authors: Benković Maja , Laljak Izvorka , Valinger Davor , Jurina Tamara , Sokač Cvetnić Tea , Gajdoš Kljusurić Jasenka , Jurinjak Tušek Ana
Published: March 2024

The aim of this work was to analyze and compare the adsorption and desorption processes of carbohydrates (glucose as a model molecule), polyphenols (gallic acid as a model molecule), and proteins (bovine serum albumin, BSA as a model molecule) on alginate microcapsules. The adsorption and desorption processes were described by mathematical models (pseudo-first-order, pseudo-second-order, and Weber–Morris intraparticle diffusion model for adsorption, and first-order, Korsmeyer–Peppas, and the Higuchi model for desorption) in order to determine the dominant mechanisms responsible for both processes. By comparing the values of adsorption rate (k2) and initial adsorption rate (h0) based on the pseudo-first-order model, the lowest values were recorded for BSA (k1 = 0.124 ± 0.030 min−1), followed by glucose (k1 = 0.203 ± 0.041 min−1), while the model-obtained values for gallic acid were not considered significant at p < 0.05. For glucose and gallic acid, the limiting step of the adsorption process is the chemical sorption of substances, and the rate of adsorption does not depend on the adsorbate concentration, but depends on the capacity of the hydrogel adsorbent. Based on the desorption rates determined by the Korsmeyer–Peppas model (k), the highest values were recorded for gallic acid (k = 3.66236 ± 0.20776 g beads/mg gallic acid per min), followed by glucose (k = 2.55760 ± 0.16960 g beads/mg glucose per min) and BSA (k = 0.78881 ± 0.11872 g beads/mg BSA per min). The desorption process from alginate hydrogel microcapsules is characterized by the pseudo Fickian diffusion mechanism.
Effect of Hydrogel Stiffness on Chemoresistance of Breast Cancer Cells in 3D Culture
Authors: Zeng Tianjiao , Chen Huajian , Yoshitomi Toru , Kawazoe Naoki , Yang Yingnan , Chen Guoping
Published: March 2024

Chemotherapy is one of the most common strategies for cancer treatment, whereas drug resistance reduces the efficiency of chemotherapy and leads to treatment failure. The mechanism of emerging chemoresistance is complex and the effect of extracellular matrix (ECM) surrounding cells may contribute to drug resistance. Although it is well known that ECM plays an important role in orchestrating cell functions, it remains exclusive how ECM stiffness affects drug resistance. In this study, we prepared agarose hydrogels of different stiffnesses to investigate the effect of hydrogel stiffness on the chemoresistance of breast cancer cells to doxorubicin (DOX). Agarose hydrogels with a stiffness range of 1.5 kPa to 112.3 kPa were prepared and used to encapsulate breast cancer cells for a three-dimensional culture with different concentrations of DOX. The viability of the cells cultured in the hydrogels was dependent on both DOX concentration and hydrogel stiffness. Cell viability decreased with DOX concentration when the cells were cultured in the same stiffness hydrogels. When DOX concentration was the same, breast cancer cells showed higher viability in high-stiffness hydrogels than they did in low-stiffness hydrogels. Furthermore, the expression of P-glycoprotein mRNA in high-stiffness hydrogels was higher than that in low-stiffness hydrogels. The results suggested that hydrogel stiffness could affect the resistance of breast cancer cells to DOX by regulating the expression of chemoresistance-related genes.
Investigating Texture and Freeze–Thaw Stability of Cold-Set Gel Prepared by Soy Protein Isolate and Carrageenan Compounding
Authors: Wang Zhuying , Yu Zhenhai , Ren Shuanghe , Liu Jun , Xu Jing , Guo Zengwang , Wang Zhongjiang
Published: March 2024

In this study, the purpose was to investigate the effects with different concentrations of carrageenan (CG, 0–0.30%) on the gel properties and freeze–thaw stability of soy protein isolate (SPI, 8%) cold-set gels. LF-NMR, MRI, and rheology revealed that CG promoted the formation of SPI-CG cold-set gel dense three-dimensional network structures and increased gel network cross-linking sites. As visually demonstrated by microstructure observations, CG contributed to the formation of stable SPI-CG cold-set gels with uniform and compact network structures. The dense gel network formation was caused when the proportion of disulfide bonds in the intermolecular interaction of SPI-CG cold-set gels increased, and the particle size and zeta potential of SPI-CG aggregates increased. SG20 (0.20% CG) had the densest gel network in all samples. It effectively hindered the migration and flow of water, which decreased the damage of freezing to the gel network. Therefore, SG20 exhibited excellent gel strength, water holding capacity, freeze–thaw stability, and steaming stability. This was beneficial for the gel having a good quality after freeze–thaw, which provided a valuable reference for the development of freeze–thaw-resistant SPI cold-set gel products.
Dynamics of Mitochondrial DNA Copy Number and Membrane Potential in Mouse Pre-Implantation Embryos: Responses to Diverse Types of Oxidative Stress
Authors: Winstanley Yasmyn E. , Liu Jun , Adhikari Deepak , Gonzalez Macarena B. , Russell Darryl L. , Carroll John , Robker Rebecca L.
Published: March 2024

Mitochondria undergo a myriad of changes during pre-implantation embryo development, including shifts in activity levels and mitochondrial DNA (mtDNA) replication. However, how these distinct aspects of mitochondrial function are linked and their responsiveness to diverse stressors is not well understood. Here, we show that mtDNA content increased between 8-cell embryos and the blastocyst stage, with similar copy numbers per cell in the inner cell mass (ICM) and trophectoderm (TE). In contrast, mitochondrial membrane potential (MMP) was higher in TE than ICM. Culture in ambient oxygen (20% O2) altered both aspects of mitochondrial function: the mtDNA copy number was upregulated in ICM, while MMP was diminished in TE. Embryos cultured in 20% O2 also exhibited delayed development kinetics, impaired implantation, and reduced mtDNA levels in E18 fetal liver. A model of oocyte mitochondrial stress using rotenone showed only a modest effect on on-time development and did not alter the mtDNA copy number in ICM; however, following embryo transfer, mtDNA was higher in the fetal heart. Lastly, endogenous mitochondrial dysfunction, induced by maternal age and obesity, altered the blastocyst mtDNA copy number, but not within the ICM. These results demonstrate that mitochondrial activity and mtDNA content exhibit cell-specific changes and are differentially responsive to diverse types of oxidative stress during pre-implantation embryogenesis.
The Effectiveness and Usability of a Rehabilitation Program Using the Nintendo Switch to Promote Healthy Aging in Older People with Cognitive Impairment: A Randomized Clinical Trial
Authors: Zegarra-Ramos Melissa Helen , García-Bravo Cristina , Huertas-Hoyas Elisabet , Fernández-Gómez Gemma , Rodríguez-Pérez Mª Pilar , Pérez-Corrales Jorge , García-Bravo Sara
Published: March 2024

(1) Background: Healthy aging is the process of developing and maintaining functional capacity and optimizing involvement in order to improve one’s quality of life as people age. This study aimed to encourage healthy aging in people with cognitive impairment, as well as a control group, via the use of the Nintendo Switch combined with occupational therapy sessions, aiming to improve gross and fine motor skills, functionality, quality of life, and cognitive status. (2) Methods: A randomized clinical trial was undertaken. The sample was randomized using the OxMar software program Attribution 4.0 International, facilitating the division into a control group (CG), who received conventional occupational therapy sessions, and an experimental group (EG), who received therapy incorporating the Nintendo Switch, in addition to conventional occupational therapy sessions. The intervention period with the Nintendo Switch lasted for 8 weeks. (3) Results: Thirty-two participants were included in the study. Significant differences were found in the vast majority of the variables analyzed, which shows an improvement following the intervention; these improvements were mainly observed in measures of skill and the perception of quality of life. (4) Conclusions: An eight-week intervention with the Nintendo Switch alongside conventional occupational therapy helps to maintain cognitive status and functional independence. Following 8 weeks of intervention with the Nintendo Switch, improvements in gross motor dexterity, fine motor dexterity, and quality of life were observed in older people with cognitive impairment.
The Healthspan Project: A Retrospective Pilot of Biomarkers and Biometric Outcomes after a 6-Month Multi-Modal Wellness Intervention
Authors: Chun Elizabeth , Crete Annie , Neal Christopher , Joseph Richard , Pojednic Rachele
Published: March 2024

Wellness-centric proactive healthcare is increasingly sought after, with individuals frequently embracing complementary modalities to achieve this goal. In this six-month study, healthy adult participants (n = 25) received specific therapies, including whole-body cryotherapy, infrared sauna, and photobiomodulation, along with guidance on physical activity, diet, and alcohol intake. Serum biomarkers were measured for all participants, while a subset also received biometric assessments for body composition (n = 10) and heart rate variability (n = 7). Over the course of the study (mean (SD) follow-up days = 174 (130)), participants exhibited significant improvements in health. LDL cholesterol (−9.77 (15.43) md/dL) and hsCRP (−1.75 (2.66) mg/L) decreased significantly (p < 0.05). HbA1c increased slightly (p < 0.05), but the effect size was small (0.12 (0.13)%). The body composition subset lost overall body weight (−3.29 (3.75) kg), primarily body fat, while preserving lean muscle mass (p < 0.05). Heart rate variability increased for those with existing cardiovascular risk factors (p < 0.05). In conclusion, participation in the multimodal Healthspan protocol is associated with substantial improvements in health-related biomarkers and biometrics.
Knowledge, Awareness, Attitudes and Practices toward Perimenopausal Symptoms among Saudi Females
Authors: Aljunaid Mohammed A. , Alruwaili Lojain Nasser , Alhajuj Hamzah Yahya , Musslem Mohammed Talal , Jamal Hussain Hasan
Published: March 2024

Women’s knowledge about perimenopause plays a crucial role in shaping their perception of related illnesses, influencing coping strategies, treatment adherence, and the overall management of this life stage. This cross-sectional study assessed the awareness, knowledge, attitudes, and practices regarding perimenopause among 409 Saudi women attending primary healthcare centers. Participants completed a structured questionnaire addressing demographic data, awareness, knowledge, attitudes, and practices related to perimenopause. While 75.3% of the participants were aware of perimenopause, only 17.4% could identify more than 10 out of 20 perimenopause symptoms. Commonly recognized symptoms included menstrual irregularity (67.7%), mood swings (66.0%), and mood fluctuations (50.4%). Only 23.0% had optimal knowledge about perimenopause complications. Additionally, 73.3% had not consulted a doctor for perimenopause-related issues. An analysis of the overall knowledge score showed a mean (SD) = 14.82 (5.64) out of 34. The level of knowledge was independently associated with a higher educational level, more frequent perimenopause symptoms, and regular doctor visits. This study reveals high awareness but insufficient knowledge among Saudi women regarding perimenopause symptoms and complications associated with higher perimenopause morbidity and a lack of engagement with healthcare professionals. It underscores the need for early and continued education on perimenopause, improved doctor–patient communication, and specific interventions to boost knowledge and attitudes toward perimenopause.
Is Craniosacral Therapy Effective? A Systematic Review and Meta-Analysis
Authors: Ceballos-Laita Luis , Ernst Edzard , Carrasco-Uribarren Andoni , Cabanillas-Barea Sara , Esteban-Pérez Jaime , Jiménez-del-Barrio Sandra
Published: March 2024

Objectives: The aim of this study was to evaluate the clinical effectiveness of craniosacral therapy (CST) in the management of any conditions. Methods: Two independent reviewers searched the PubMed, Physiotherapy Evidence Database, Cochrane Library, Web of Science, and Osteopathic Medicine Digital Library databases in August 2023, and extracted data from randomized controlled trials (RCT) evaluating the clinical effectiveness of CST. The PEDro scale and Cochrane Risk of Bias 2 tool were used to assess the potential risk of bias in the included studies. The certainty of the evidence of each outcome variable was determined using GRADEpro. Quantitative synthesis was carried out with RevMan 5.4 software using random effect models. Data Synthesis: Fifteen RCTs were included in the qualitative and seven in the quantitative synthesis. For musculoskeletal disorders, the qualitative and quantitative synthesis suggested that CST produces no statistically significant or clinically relevant changes in pain and/or disability/impact in patients with headache disorders, neck pain, low back pain, pelvic girdle pain, or fibromyalgia. For non-musculoskeletal disorders, the qualitative and quantitative synthesis showed that CST was not effective for managing infant colic, preterm infants, cerebral palsy, or visual function deficits. Conclusions: The qualitative and quantitative synthesis of the evidence suggest that CST produces no benefits in any of the musculoskeletal or non-musculoskeletal conditions assessed. Two RCTs suggested statistically significant benefits of CST in children. However, both studies are seriously flawed, and their findings are thus likely to be false positive.
Pollutants in Breast Milk: A Scoping Review of the Most Recent Data in 2024
Authors: Serreau Raphaël , Terbeche Yasmine , Rigourd Virginie
Published: March 2024

Perinatal exposure to pollutants, including persistent organic pollutants (POPs) and heavy metals, poses significant risks to both mothers and children, marking this period as highly vulnerable. Despite the well-acknowledged benefits of breastfeeding, there exists a gap in comprehensive understanding regarding the impact of environmental pollutants on breast milk, underscoring the critical need for this study. Our research addresses this gap by exploring the intersection of environmental health and lactation, situated within the broader ‘One Health’ concept, thus contributing a novel perspective to the existing body of knowledge. This scoping review aims to examine recent research on the persistent presence of organic pollutants (POPs) and heavy metals in breast milk, thereby elucidating the environmental setting’s impact on milk quality. We seek to highlight the innovative angle of our study by emphasizing the ‘One Health’ concept, which has not been thoroughly explored in the context of lactation and environmental pollutants. We performed a scoping review, consulting two online databases to identify articles published from 1995 to 2023 that reported on pollutants in breast milk, using the PRISMA checklist. This methodological approach underlines the comprehensive and up-to-date nature of our literature review, ensuring the relevance and timeliness of our findings. From a total of 54 relevant articles, findings indicate that POPs are present in higher concentrations in breast milk the longer the lactation period. These findings highlight the persistent and bioaccumulative nature of such contaminants, offering new insights into their long-term implications for maternal and infant health. This exposure does not appear time-sensitive, suggesting pollutants accumulated in maternal fat compartments can be excreted into human milk years after exposure, a novel finding that underscores the importance of considering long-term environmental exposures in lactation research. The presence of POPs and heavy metals in both infant formula and maternal milk underscores a critical need for further comparative studies to understand the health implications better. Our discussion extends the current dialogue on the safety of breastfeeding in polluted environments, providing a new framework for assessing risks and benefits. While breastfeeding remains the WHO-recommended nutrition for optimal infant growth, the findings emphasize the importance of continued risk reduction policies to protect mothers and infants from environmental contaminants in breast milk. Our conclusion calls for an integrated approach, combining public health, environmental science, and clinical practice to develop effective strategies for reducing exposure to environmental pollutants. This multidisciplinary perspective is a significant contribution to the field, paving the way for future research and policy development.
Insights into Shared Decision-Making in Interprofessional Teams for a Boy with Down Syndrome with Communication and Language Issues: Simulation-Based Training for Medical and Allied Health Students
Authors: Deckers Stijn R. J. M. , van Zaalen Yvonne
Published: March 2024

Background: Shared decision-making is one key element of interprofessional collaboration. Communication is often considered to be the main reason for inefficient or ineffective collaboration. Little is known about group dynamics in the process of shared decision-making in a team with professionals, including the patient or their parent. This study aimed to evaluate just that. Methods: Simulation-based training was provided for groups of medical and allied health profession students from universities across the globe. In an overt ethnographic research design, passive observations were made to ensure careful observations and accurate reporting. The training offered the context to directly experience the behaviors and interactions of a group of people. Results: Overall, 39 different goals were defined in different orders of prioritizing and with different time frames or intervention ideas. Shared decision-making was lacking, and groups chose to convince the parents when a conflict arose. Group dynamics made parents verbally agree with professionals, although their non-verbal communication was not in congruence with that. Conclusions: The outcome and goalsetting of an interprofessional meeting are highly influenced by group dynamics. The vision, structure, process, and results of the meeting are affected by multiple inter- or intrapersonal factors.
Mortars and Renders: The Case of the Roman Villa Horta da Torre Fronteira, Portugal
Authors: Ditta A. , Carneiro André , Galacho Cristina , Moita Patrícia
Published: March 2024

The archaeological site of villa Horta da Torre in Portugal reveals distinctive architectural features within the context of Roman villae in Hispania. Notably, the triclinium was designed with an artificial cascade originating from a double apse wall, and the walls were adorned with mosaic tessellae panels and marble skirting. During the Roman era, the surrounding area belonged to the former province of Lusitania, with Augusta Emerita serving as its capital. This study examines 11 mortar samples from various contexts and functions, such as masonry, preparatory, render, and opus signinum mortars. A set of complementary analytical techniques was employed to determine the textural and mineralogical compositions of the mortars. The aim was to gain insights into the production techniques and the selection of raw materials within the geological context of this rural construction. It was observed that the processing of raw materials and production techniques did not always adhere to the rules of Vitruvius. A comparison with other villas revealed comparatively less meticulous attention to sand selection and precise layering for mural painting. Nevertheless, it was noted that in preparatory layers for the supranucleus and nucleus tesserae, ceramic pieces were intentionally added to improve the mortar. The binder used was calcitic lime, likely obtained from locally sourced limestone. The nature of aggregates is diversified but consistent with the local geological provenance within a radius of less than 3 km, in accordance with two sand exploration sites. This research aligns with the United Nations’ 2030 Agenda for Sustainable Development, contributing specifically to SDG 11 and Target 11.4, which “aims to strengthen efforts to protect and safeguard the world’s cultural and natural heritage”.
Seed Morphological Analysis in Species of Vitis and Relatives
Authors: Martín-Gómez José Javier , Rodríguez-Lorenzo José Luis , Gutiérrez del Pozo Diego , Cabello Sáez de Santamaría Félix , Muñoz-Organero Gregorio , Tocino Ángel , Cervantes Emilio
Published: March 2024

Seed shape descriptions of species of Vitis have traditionally been based on adjectives comparing overall shape with geometric figures, such as oval, elongated oval, and pear-shaped, corresponding to higher values of the Stummer index (lower aspect ratio) for oval, and lower values of the Stummer index for pear shape (or elongated seeds, with a higher aspect ratio). Analytical, quantitative descriptions of shape have recently been applied to diverse genera of Vitaceae and cultivated varieties of Vitis. Here, we present the application of three quantitative methods to the seed shape description of ten species of the genus Vitis and three species of related genera (Ampelopsis, Cissus and Parthenocissus). First, general seed shape was described through comparisons using geometric models. For this, the average silhouettes of representative seed populations were used as models for shape quantification. Two additional quantitative methods were based on the measurement of bilateral symmetry and curvature analysis in the apex. Quantitative methods for shape description based on similarity with the models give an accurate account of the relationships between Vitis species. The resulting dendrogram is like the dendrogram obtained from a combined analysis using the data from general measurements and curvature and symmetry analyses. The original methods presented here for seed morphology are useful for analyzing the phylogenetic relationships between species of Vitis.
Integrative Phytohormone and Transcriptome Analyses Reveal the Inhibitory Mechanism of Ethylene on Potato Tuber Sprouting at Room Temperature
Authors: Zhang Meixue , Jiao Wenxiao , Chen Qingmin , Fu Maorun , Han Cong
Published: March 2024

As a commercial potato sprout suppressant, ethylene (Eth) is usually used under a low temperature for long-term storage of potato tubers. However, in many cases, potato tubers are usually transferred from a refrigeration house and sold at room temperature. In the present research, Eth’s inhibitory effects on tuber sprouting at room temperature were investigated. The potential molecular mechanisms of Eth-induced sprout suppression were revealed by phytohormone and transcriptome analyses. Results showed that exogenous Eth significantly suppressed sprout growth in potato tubers during two weeks of storage. The endogenous plant hormone levels of abscisic acid (ABA) and auxin (Aux) were markedly reduced by Eth treatment. Transcriptomic analysis revealed that more transcriptional changes occurred in the early stage of sprouting. The differentially expressed genes (DEGs) assigned to the pathways of plant hormone signal transduction, photosynthesis, starch and sucrose metabolism and phenylpropanoid biosynthesis, which were closely associated with sprouting, were extensively down-regulated by Eth treatment. In addition, the heat map of six hormone signal transduction pathways showed that the expression of most DEGs annotated into the Aux signal transduction pathway was suppressed by Eth treatment, while the expression of many DEGs annotated as ETR (Eth receptor) and ERF1/2 (Eth-responsive transcription factor 1 and 2) in the Eth signal transduction pathway was enhanced by Eth treatment. Taken together, our results indicated that Eth-induced sprout inhibition might be closely related to the suppression of internal Aux production and signal transduction and the activation of Eth signal transduction.
Comparison of the Differences in Tolerance to Drought Stress across Five Clematis Species Based on Seed Germination and Seedling Growth
Authors: Zhu Zhu , Li Yongmei , Liu Ting , Shi Rongrong , Xu Xiangyun , Song Zhanbang , Wang Yanli
Published: March 2024

Plants of the Clematis genus are attractive ornamental plants due to their various flower colors and patterns, and they play an important role as ground cover plants in landscaping. However, the application of Clematis plants in landscaping in arid and semi-arid areas is limited. This study used five common wild Clematis species in Gansu Province as experimental materials, including Clematistangutica, Clematis glauca, Clematis intricata, Clematis nannophylla, and Clematis fruticosa. By simulating different intensities of drought stress with polyethylene glycol (PEG), the germination behavior of seeds and the physiological and biochemical responses of seedlings of these five species to drought stress were observed. The results showed that 15% PEG stress significantly inhibited the seed germination of the five species, which was also the drought threshold for seed germination of C. fruticosa. C. tangutica exhibited the strongest tolerance to drought stress in seed germination. Seedlings of the five Clematis plants showed different levels of tolerance to drought stress, mainly demonstrating higher tolerance to 10% and 20% concentrations of PEG stress, while a 30% concentration of PEG stress caused varying degrees of damage to the seedlings of the five Clematis species. PCA analysis indicated that seedlings of C. intricata and C. glauca had higher scores under drought stress. These findings can provide a theoretical basis for the selection of urban landscaping plant species in arid and semi-arid regions of northwest China.
Effect of Seed Spaceflight Storage on Tomato Fruit Quality and Peel/Pulp Mineral and Antioxidant Distribution
Authors: Golubkina Nadezhda , Dzhos Elena , Bogachuk Maria , Antoshkina Marina , Verba Olga , Zavarykina Tatiana , Nechitailo Galina , Murariu Otilia Cristina , Tallarita Alessio Vincenzo , Caruso Gianluca
Published: March 2024

The spaceflight storage of seeds is known to cause mutations affecting both their quality and the mature plants originating from them. To study the effects of space stress, tomato seeds of two cultivars (Lotus and Autumn rhapsody) were subjected to half a year of storage at the International Space Station (ISS), and then, sown in a greenhouse to produce tomato fruits. The space-treated plants gave smaller fruits with a stable total yield not significantly different from that of the control plants. Space-treated tomatoes showed significantly higher levels of dry matter, dietary fiber, monosaccharides and citric and malic acids and lower values of oxalic acid compared to the control plants. The pulp of space-treated fruits had 1.44–1.70 times lower levels of carotenoids, while their peel contained a 1.27–1.90 times higher pigment amount compared to the control plants. No significant changes in the total antioxidant activity (AOA), photosynthetic pigments and phenolic (TP) and proline content were recorded in the fruits due to seed spaceflight storage. Contrarily, space-treated tomatoes showed decreased levels of Ca, Sr and Mo and increased Se both in the fruit pulp and peel. The concentration of Fe and especially Pb was lower in space-treated fruit pulp. Positive correlations between Se and dry matter, Ca and Sr, Ca and Co, Ca and Fe, and Cr and carotenoids, and negative correlations between Se and Mo, Se and K, and Mo and dry matter were recorded. The results indicate that seed stress caused by long-term spaceflight affects both the biochemical characteristics and mineral composition of tomato fruits and causes the peel/pulp redistribution of carotenoids as well as macro- and micro-elements, improving Se accumulation levels in the fruit peel.
Physiological and Transcriptional Characteristics of Banana Seedlings in Response to Nitrogen Deficiency Stress
Authors: Zhao Lei , Cai Bingyu , Zhang Xiaohan , Zhang Bencheng , Feng Junting , Zhou Dengbo , Chen Yufeng , Zhang Miaoyi , Qi Dengfeng , Wang Wei , Xie Jianghui , Wei Yongzan
Published: March 2024

Nitrogen is a crucial element for the growth and development of plants, directly affecting crop growth and yield. To investigate the physiological and molecular mechanism of nitrogen-deficiency stress, we conducted an investigation into the effects of different nitrogen levels on the growth, photosynthetic characteristics, and gene transcription levels of banana seedlings. Compared with the control group with normal nitrogen levels (NN), the height of plants receiving Reduced-N (NR), Low-N (LN), and N-Free (NF) treatments was decreased by 0.45 cm, 2.5 cm, and 3.25 cm, respectively. Their dry weight was reduced by 1.63 g, 2.99 g, and 2.88 g, respectively. Conversely, the dry weight of the underground plant part in the LN and NF treatment groups exhibited an increase of 0.13 g and 0.16 g, respectively. Regarding photosynthetic characteristics, the Specialty Products Agricultural Division (SPAD) values of the NR, LN, and NF treatments showed reductions of 15.5%, 30.4%, and 35.9%, respectively, compared with those of the control treatments. The values of maximum photosynthetic efficiency (Fv/Fm), actual photosynthetic efficiency (Y(Ⅱ)), and relative electron transfer (ETR) of the banana seedlings decreased to different degrees after NR, LN, and NF treatment, and their values were positively correlated with N levels. Gene transcription analysis showed that N transport-related proteins, including NRT1.7, NRT2.3a, NRT2.3b, and NRT2.5, were significantly up-regulated to increase the nitrogen absorption capacity of plant roots. On the other hand, various transcription factors including GRAS, MYB, and WRKY were notably up-regulated, facilitating root growth and the expanding root absorption area, thereby enhancing nitrogen uptake. Furthermore, genes associated with endogenous hormone metabolic pathways such as gibberellin (GA), strigolactone (SL), and brassinosteroids (BR) were activated in banana plants subjected to low nitrogen stress, enhancing the plant’s ability to adapt to nitrogen-deficient conditions. These findings offer valuable insights into understanding the transcriptional regulatory mechanisms governing banana responses to low nitrogen stress and breeding new varieties with improved nutrient utilization.