bluuebunny/crossref_metadata_2025 huggingface dataset:
A Quality Improvement Initiative to Reduce Postoperative Delirium among Cardiac Surgery Patients
Authors: Sanjanwala Rohan M. , Hiebert Brett , Kent David , Warren Sandy , Grocott Hilary , Arora Rakesh C.
Published: November 2021

Patients following cardiac surgery commonly experience post-operative delirium (POD) during their postoperative hospital stay. A multifaceted, specialty wide, quality improvement (QI) project was undertaken for patients experiencing POD. The goal was to develop a reduction in POD care bundle (rPOD-a structured patient care program) that encompasses efficient preoperative risk factor identification and a postoperative patient-care process to ensure early POD identification and treatment. The following steps were taken to implement the rPOD care bundle including: (a) Developing a quality driven, evidence-based guideline for the perioperative cardiac surgery health care team, (b) identifying and addressing local barriers to implementation, (c) selecting performance measures to assess intervention adherence and patient outcomes, and (d) ensuring that all patients receive the interventions through staff engagement and education, and regular project evaluation. Trends of process measures and quality improvement measures were examined. An increasing trend in the rate of postoperative delirium screening during implementation of rPOD intervention was demonstrated. This quality improvement study provides a bases for future postoperative delirium reduction interventions.
Reference Values for External and Internal Training Intensity Monitoring in Young Male Soccer Players: A Systematic Review
Authors: Oliveira Rafael , Brito João Paulo , Moreno-Villanueva Adrián , Nalha Matilde , Rico-González Markel , Clemente Filipe Manuel
Published: November 2021

Training intensity monitoring is a daily practice in soccer which allows soccer academies to assess the efficacy of its developmental interventions and management strategies. The current systematic review’s purpose is to: (1) identify and summarize studies that have examined external and internal training intensity monitoring, and to (2) provide references values for the main measures for young male soccer players. A systematic review of EBSCO, PubMed, Scielo, Scopus, SPORTDiscus, and Web of Science databases was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. From the 2404 studies initially identified, 8 were fully reviewed, and their outcome measures were extracted and analyzed. From them, the following range intervals were found for training: rated perceived exertion (RPE) 2.3–6.3 au; session-RPE, 156–394 au; total distance, 3964.5–6500 m and; distance >18 km/h, 11.8–250 m. Additionally, a general tendency to decrease the intensity in the day before the match was Found. This study allowed to provide reference values of professional young male players for the main internal and external measures. All together, they can be used by coaches, their staff, or practitioners in order to better adjust training intensity.
Usability of Telemedicine Mobile Applications during COVID-19 in Saudi Arabia: A Heuristic Evaluation of Patient User Interfaces
Authors: Aldekhyyel Raniah N. , Almulhem Jwaher A. , Binkheder Samar
Published: November 2021

The coronavirus disease 2019 (COVID-19) pandemic has impacted the use of telemedicine application (apps), which has seen an uprise. This study evaluated the usability of the user interface design of telemedicine apps deployed during the COVID-19 pandemic in Saudi Arabia. It also explored changes to the apps’ usability based on the pandemic timeline. Methods: We screened ten mHealth apps published by the National Digital Transformation Unit and selected three telemedicine apps: (1) governmental “Seha”® app, (2) stand-alone “Cura”® app, and (3) private “Dr. Sulaiman Alhabib”®app. We conducted the evaluations in April 2020 and in June 2021 by identifying positive app features, using Nielsen’s ten usability heuristics with a five-point severity rating scale, and documenting redesign recommendations. Results: We identified 54 user interface usability issues during both evaluation periods: 18 issues in “Seha” 14 issues in “Cura”, and 22 issues in “Dr. Sulaiman Alhabib”. The two most heuristic items violated in “Seha”, were “user control and freedom” and “recognition rather than recall”. In “Cura”, the three most heuristic items violated were “consistency and adherence to standards”, “esthetic and minimalist design”, and “help and documentation” In “Dr. Sulaiman Alhabib” the most heuristic item violated was “error prevention”. Ten out of the thirty usability issues identified from our first evaluation were no longer identified during our second evaluation. Conclusions: our findings indicate that all three apps have a room for improving their user interface designs to improve the overall user experience and to ensure the continuity of these services beyond the pandemic.
A Potential Application of Endophytic Bacteria in Strawberry Production
Authors: Mei Chuansheng , Amaradasa B. Sajeewa , Chretien Robert L. , Liu Danyang , Snead George , Samtani Jayesh B. , Lowman Scott
Published: November 2021

Endophytic bacteria could be used as a tool to promote plant growth, enhance abiotic stress tolerance, and inhibit plant pathogen growth. More than 100 bacteria, previously tested to have biocontrol activity against fungal pathogens from our lab, were chosen for pathogen inhibition in vitro with antibiosis assays against strawberry specific pathogens. Three potential endophytic bacteria were tested in the greenhouse for disease reduction and growth performance. Finally, field trials were conducted for fruit yield production. Our results showed that three endophytic bacteria significantly inhibited the growth of the strawberry pathogen Colletotrichum gloeosporioides from antibiosis assays and were identified as Bacillus velezensis strains IALR308, IALR585, and IALR619. Inoculation of these bacteria significantly reduced strawberry disease in greenhouse conditions with pathogen infection. Field trials showed that IALR619 has potential to influence marketable fruit yield when strawberry plants were inoculated twice over the growing season. All three bacteria had the ability to produce auxin and to solubilize phosphate. The antibiotics surfactin and iturin were also detected in IALR585 and IALR619. In conclusion, Bacillus velezensis IALR619 has potential inhibition of strawberry pathogen growth in the greenhouse and possible ability to increase marketable fruit yield in the field.
The Urban Double-Crop: Can Fall Vegetables and a Warm-Season Lawn Co-Exist?
Authors: Bauske Ellen M. , Dorn Sheri , Waltz Freddie Clinton , Garcia Chance Lauren
Published: November 2021

A gardening methodology using double-cropped cool-season vegetables and warm-season turfgrass, thereby capitalizing on the ideal growing season for each, was developed in field trials and tested in volunteers’ landscapes. Broccoli (Brassica oleracea), lettuce (Lactuca sativa), and Swiss chard (Beta vulgaris subsp. Cicla) were planted into an established hybrid bermudagrass lawn (Cynodon dactylon (L.) Pers. × C. transvaalensis Burtt-Davy ‘Tifsport’) in September. The vegetables were planted into tilled strips, 5 cm × 10 cm holes and 10 cm × 10 cm holes in the turf. All treatments produced harvestable yield, though the yield of vegetables planted in the tilled treatments and larger holes was greater than in smaller holes. Efforts to reduce turfgrass competition with vegetables by the application of glyphosate or the use of the Veggie Lawn Pod (an easily installed plastic cover on the lawn) did not increase yield. Tilled treatments left depressions that discouraged spring turfgrass recovery. The double-crop was tested by seven volunteers on their lawns. Though lawn-planted vegetables did not produce as much yield as those planted in the volunteers’ gardens, the volunteers were enthusiastic about this methodology. The volunteers reported that lawn vegetables were more difficult to plant but not more difficult to maintain, and they were easier to harvest than vegetables in their gardens. All volunteers reported satisfactory recovery of their lawns in the spring.
Land Use Evolution and Land Ecological Security Evaluation Based on AHP-FCE Model: Evidence from China
Authors: Zhu Yong , Zhong Shihu , Wang Ying , Liu Muhua
Published: November 2021

China experienced rapid urbanization and socioeconomic development at an unusual rate during the past four decades. Against such background, land use evolution and land ecological security have both been affected in a volatile way. Therefore, it is necessary to investigate the land use and the land ecological security in China. However, the traditional assessment approaches have paid more attention to the environmental and economic factors than the sustainable development of ecology, which cannot comprehensively assess the land ecological security. From the perspective of ecological sustainable development, this study identifies 3 main factors and 17 sub-factors. We also construct a model to integrate the FCE approach with the AHP. The results show that from 2004 to 2017, China’s land use structure was unbalanced. The construction land, mining land, and cultivated land increased rapidly, leading to the shrinkage of ecological land. Moreover, the weight of the sustainable development of resources and the environment, economic sustainable development, social sustainable development are 0.3341, 0.3780, and 0.2879, respectively, demonstrating that economic sustainable development is the most important factor affecting land ecological security. Finally, although the value of comprehensive land ecological security in China has been on the rise from 2004 to 2017, it remains at an unsecured level. Moreover, the value of the sustainable development of resources and the environment has been declining since 2011 and is lower than the values of economic sustainable development and social sustainable development. This study demonstrates that more attention should be paid to enhancing land ecological security, especially promoting the sustainable development of resources and the environment.
An Investigation of the Initial Recovery Time of Chinese Enterprises Affected by COVID-19 Using an Accelerated Failure Time Model
Authors: Yang Lijiao , Qi Yishuang , Jiang Xinyu
Published: November 2021

COVID-19 has had a great impact on the economy, society, and people’s lives in China and globally. The production and operations of Chinese enterprises have also faced tremendous challenges. To understand the economic impact of COVID-19 on enterprises and the key affecting factors, this study adds to the literature by investigating the business recovery process of enterprises from the micro perspective. Specific attention is paid to the initial stage of business recovery. A questionnaire survey of 750 enterprises explored the impact during the pandemic period from July to September 2020. An accelerated failure time model in survival analysis was adopted to analyze the data. The results show that the manufacturing industry is mainly faced by affecting factors such as enterprise ownership, employees’ panic and order cancellation on initial enterprise recovery. As for the non-manufacturing industry, more factors, including clients’ distribution, employees’ panic, raw material shortage, cash flow shortage and order cancellation, are found to be significant. Acceleration factors that estimate the effects of those covariates on acceleration/deceleration of the recovery time are presented. For instance, the acceleration factor of employees’ panic is 1.319 for non-manufacturing, which implies that, compared with enterprises where employees are less panicked, enterprises with employees obviously panicked will recover 1.319 times slower at any quantile of probability of recovery time. This study provides a scientific reference for the post-pandemic recovery of enterprises, and can support the formulation of government policies and enterprise decisions.
Perceived Competence in the Face of Death before and after Nursing Studies: An Intrasubject Longitudinal Study
Authors: Sáez-Alvarez Enrique , Medrano-Abalos Pilar , Cunha-Pérez Cristina , Cuesta-Fernández Jesús , Martín-Utrilla Salvador
Published: November 2021

Perceived competence is a subject’s perception of being able to interact effectively with the environment. Perceived Competence in the face of death in Nursing degree programs in which the presence of the subject of death and bereavement is key becomes more topical and relevant. The objective of this study is to determine whether this competence is improved through Nursing Studies. This study was designed as paired repeated intrasubject measures, initial measurement at the beginning of the first year and second measurement in the fourth year of the Nursing degree. One hundred and seventeen nursing students were assessed. Significant improvement is evidenced in three of the four dimensions of Perceived Competence in the face of death (Accompaniment and Communication: 25.70/29.34; Self-Confidence: 9.64/12.78; Management of Self-Fear: 13.18/14.66). These results show notable differences with their cross-sectional predecessors, suggesting the need for further studies in this field to consolidate a still developing body of knowledge.
The Mediating Effect of Coping Style on Physical Activity and Negative Affect Caused by Public Health Emergencies: Evidence from Chinese College Students
Authors: Liu Yuetao , Wang Zhiyuan , You Songhui
Published: November 2021

In this study, we explored the relationship between physical activity (PA), coping style (CS) and negative affect caused by public health emergencies (PHENA), to examine if CS can play a mediating role between PA and PHENA, and analyzed the different effects of positive coping (PC) and negative coping (NC). Using the method of random sampling, 700 students from four universities in Beijing and Shanghai were recruited to complete questionnaires about PA, CS and PHENA. Data collection was conducted through online and offline questionnaires. Firstly, there is a significant correlation between PA, CS and PHENA. Secondly, PA can negatively predict PHENA, and PA has positive impact on PC and negative impact on NC. Thirdly, the mediating role of NC related to PHENA is significant, the mediating role of PC related to PHENA is not significant. College students’ participation in physical activity can reduce the probability of adopting negative coping mechanisms, thereby alleviating the PHENA.
Reframing Sexual Health for Black Girls and Women in HIV/STI Prevention Work: Highlighting the Role of Identity and Interpersonal Relationships
Authors: Opara Ijeoma , Abrams Jasmine A. , Cross Kristina , Amutah-Onukagha Ndidiamaka
Published: November 2021

While Black girls and women are disproportionately impacted by sexual health disparities, there continues to be an overwhelming focus on individual risk behaviors within prevention initiatives, which offers a fragmented narrative of the multidimensional nature of risk and plausibly limits effectiveness of prevention programs and attenuates reductions in disparities. Because sexual health is experienced within an individual’s beliefs/values, interpersonal relationships, and behaviors and reflects larger social and cultural systems, it is important to critically examine common theories used to inform HIV/STI prevention interventions for Black women and girls. To fill this gap in the literature, we critique two commonly used theories in HIV/STI prevention interventions, namely the social cognitive theory and the theory of gender and power, by highlighting theoretical and practical strengths and weaknesses. We propose research implications that incorporate key strengths of the two theories while adding new concepts grounded in the intersectionality theory. The overall goal is to introduce a more comprehensive conceptual model that is reflective of and applicable to the multidimensional sexual experiences of Black girls and women within the evolving definition of sexual health and behavior.
Not All Remote Workers Are Similar: Technology Acceptance, Remote Work Beliefs, and Wellbeing of Remote Workers during the Second Wave of the COVID-19 Pandemic
Authors: Donati Simone , Viola Gianluca , Toscano Ferdinando , Zappalà Salvatore
Published: November 2021

Although a large part of the world’s workforce engaged in mandatory Work from Home during the COVID-19 pandemic, the experience was not the same for everyone. This study explores whether different groups of employees, based on their work and organizational characteristics (i.e., organizational size, number of days per week working from home, working in team) and personal characteristics (i.e., remote work experience, having children at home), express different beliefs about working remotely, acceptance of the technology necessary to Work from Home, and well-being. A study was conducted with 163 Italian workers who answered an online questionnaire from November 2020 to January 2021. A cluster analysis revealed that work, organizational, and personal variables distinguish five different types of workers. ANOVA statistics showed that remote workers from big companies who worked remotely several days a week, had experience (because they worked remotely before the national lockdowns), and worked in a team, had more positive beliefs about working remotely, higher technology acceptance, and better coping strategies, compared to the other groups of workers. Practical implications to support institutional and organizational decision-makers and HR managers to promote remote work and employee well-being are presented.
Associations of COVID-19 Knowledge and Risk Perception with the Full Adoption of Preventive Behaviors in Seoul
Authors: Choo Jina , Park Sooyeon , Noh Songwhi
Published: November 2021

This study explores the levels of COVID-19 knowledge, risk perception, and preventive behavior practice in Seoul, to determine whether knowledge and risk perception are significantly associated with the full adoption of preventive behaviors, for the delivery of a customized public campaign to Seoul’s citizens. A total of 3000 Seoul residents participated in this study through an online questionnaire survey. They had a mean score of 84.6 for COVID-19 knowledge (range: 0–100 points) and 4.2 (range: 1–7 points) for risk perception. Of the participants, 33.4% practiced full adoption of all three preventive behaviors: hand hygiene, wearing a face mask, and social distancing; wearing a face mask was practiced the most (81.0%). Women significantly adopted these three preventive behaviors more often compared with men. Both COVID-19 knowledge and risk perception were found to be significantly associated with the full adoption of preventive behaviors; however, this association differed by the type of preventive behavior. This indicates that city-level information on the levels of COVID-19 knowledge, risk perception, and preventive behaviors should be clearly and periodically communicated among public officers and healthcare professionals to continually raise the public’s awareness of the full adoption of non-pharmaceutical preventive behaviors.
Mediating Effects of Emotional Support Reception and Provision on the Relationship between Group Interaction and Psychological Well-Being: A Study of Young Patients
Authors: Ngai Steven Sek-yum , Cheung Chau-Kiu , Mo Jianhong , Chau Spencer Yu-hong , Yu Elly Nga-hin , Wang Lin , Tang Hon-yin
Published: November 2021

While it is well-established that mutual aid groups are effective in the psychological rehabilitation of vulnerable individuals, few studies have thoroughly investigated the dynamic mechanism of how psychological well-being improves through mutual aid groups of young patients with chronic health conditions. In connection with several existing theories (i.e., the helper therapy principle, equity theory, the norm of reciprocity, and the concept of communal relationships), this study aims to: (1) evaluate whether emotional support exchanges (i.e., emotional support reception and provision) mediate the relationship between group interaction and psychological well-being; and (2) compare three potential underlying mechanisms—the mediating role of emotional support provision, equitable reciprocity (i.e., a balance of receiving and providing emotional support, where no party over-benefits or under-benefits), and sequential reciprocity (i.e., repaying the helper or a third party in the future after receiving help)—through a path analysis model. A stratified random sampling procedure with chronic health conditions as the stratifying criterion was used to recruit 391 individuals aged 12–45 years from mutual aid groups in Hong Kong, who completed both the baseline and follow-up surveys over a 12-month interval. The results of the path model revealed significant mediating roles of emotional support provision and sequential reciprocity, not equitable reciprocity. The present study offers theoretical and practical implications for promoting the psychological well-being of young patients with chronic health conditions.
Research on Public Environmental Perception of Emotion, Taking Haze as an Example
Authors: Bao Qiang , Zhang Xujuan , Wu Xijuan , Zhang Qiang , Chen Jinshou
Published: November 2021

Ecological and environmental problems have become increasingly prominent in recent years. Environmental problems represented by haze have become a topic that affects the harmonious ecology of human beings. The trend of this topic is on the rise. People’s perception of the environment after the impact of haze has also changed. A real-time grasp of the dynamic public environment perception of emotions is often an important basis for environmental management departments to effectively solve environmental problems through public opinion. This article focuses on the problem of the public perception of emotional changes, which is caused by fog and hazy weather, proposes an environmental emotion perception model, using Weibo comment data about fog and haze as environmental perception data, and analyzes the impact of fog and haze on the public in four seasonal time dimensions. The post-environment perception of emotion changes: the results show that in spring, the public’s environmental perception of emotions is mainly negative emotions at the beginning of the season; in summer, positive emotions become dominant emotions; in autumn, the public’s environmental perception of emotions is dominated by negative emotions that increase substantially; and in winter, the dominant environmental perception of emotions of the public is still negative. This theory provides support for research on social emotions and public opinion behavior.
D-Xylose Sensing in Saccharomyces cerevisiae: Insights from D-Glucose Signaling and Native D-Xylose Utilizers
Authors: Brink Daniel P. , Borgström Celina , Persson Viktor C. , Ofuji Osiro Karen , Gorwa-Grauslund Marie F.
Published: November 2021

Extension of the substrate range is among one of the metabolic engineering goals for microorganisms used in biotechnological processes because it enables the use of a wide range of raw materials as substrates. One of the most prominent examples is the engineering of baker’s yeast Saccharomyces cerevisiae for the utilization of d-xylose, a five-carbon sugar found in high abundance in lignocellulosic biomass and a key substrate to achieve good process economy in chemical production from renewable and non-edible plant feedstocks. Despite many excellent engineering strategies that have allowed recombinant S. cerevisiae to ferment d-xylose to ethanol at high yields, the consumption rate of d-xylose is still significantly lower than that of its preferred sugar d-glucose. In mixed d-glucose/d-xylose cultivations, d-xylose is only utilized after d-glucose depletion, which leads to prolonged process times and added costs. Due to this limitation, the response on d-xylose in the native sugar signaling pathways has emerged as a promising next-level engineering target. Here we review the current status of the knowledge of the response of S. cerevisiae signaling pathways to d-xylose. To do this, we first summarize the response of the native sensing and signaling pathways in S. cerevisiae to d-glucose (the preferred sugar of the yeast). Using the d-glucose case as a point of reference, we then proceed to discuss the known signaling response to d-xylose in S. cerevisiae and current attempts of improving the response by signaling engineering using native targets and synthetic (non-native) regulatory circuits.
3D Printed Multiphasic Scaffolds for Osteochondral Repair: Challenges and Opportunities
Authors: Doyle Stephanie E. , Snow Finn , Duchi Serena , O’Connell Cathal D. , Onofrillo Carmine , Di Bella Claudia , Pirogova Elena
Published: November 2021

Osteochondral (OC) defects are debilitating joint injuries characterized by the loss of full thickness articular cartilage along with the underlying calcified cartilage through to the subchondral bone. While current surgical treatments can provide some relief from pain, none can fully repair all the components of the OC unit and restore its native function. Engineering OC tissue is challenging due to the presence of the three distinct tissue regions. Recent advances in additive manufacturing provide unprecedented control over the internal microstructure of bioscaffolds, the patterning of growth factors and the encapsulation of potentially regenerative cells. These developments are ushering in a new paradigm of ‘multiphasic’ scaffold designs in which the optimal micro-environment for each tissue region is individually crafted. Although the adoption of these techniques provides new opportunities in OC research, it also introduces challenges, such as creating tissue interfaces, integrating multiple fabrication techniques and co-culturing different cells within the same construct. This review captures the considerations and capabilities in developing 3D printed OC scaffolds, including materials, fabrication techniques, mechanical function, biological components and design.
Genome-Wide Analysis of the Apple CBL Family Reveals That Mdcbl10.1 Functions Positively in Modulating Apple Salt Tolerance
Authors: Chen Peihong , Yang Jie , Mei Quanlin , Liu Huayu , Cheng Yunpeng , Ma Fengwang , Mao Ke
Published: November 2021

Abiotic stresses are increasingly harmful to crop yield and quality. Calcium and its signaling pathway play an important role in modulating plant stress tolerance. As specific Ca2+ sensors, calcineurin B-like (CBL) proteins play vital roles in plant stress response and calcium signaling. The CBL family has been identified in many plant species; however, the characterization of the CBL family and the functional study of apple MdCBL proteins in salt response have yet to be conducted in apple. In this study, 11 MdCBL genes were identified from the apple genome. The coding sequences of these MdCBL genes were cloned, and the gene structure and conserved motifs were analyzed in detail. The phylogenetic analysis indicated that these MdCBL proteins could be divided into four groups. The functional identification in Na+-sensitive yeast mutant showed that the overexpression of seven MdCBL genes could confer enhanced salt stress resistance in transgenic yeast. The function of MdCBL10.1 in regulating salt tolerance was also verified in cisgenic apple calli and apple plants. These results provided valuable insights for future research examining the function and mechanism of CBL proteins in regulating apple salt tolerance.
Indole-3-Propionic Acid, a Functional Metabolite of Clostridium sporogenes, Promotes Muscle Tissue Development and Reduces Muscle Cell Inflammation
Authors: Du Lei , Qi Renli , Wang Jing , Liu Zuohua , Wu Zhenlong
Published: November 2021

Clostridium sporogenes (C. sporogenes), as a potential probiotic, metabolizes tryptophan and produces an anti-inflammatory metabolite, indole-3-propionic acid (IPA). Herein, we studied the effects of C. sporogenes and its bioactive metabolite, IPA, on skeletal muscle development and chronic inflammation in mice. In the in vivo study, the muscle tissues and serum samples of mice with C. sporogenes supplementation were used to analyze the effects of C. sporogenes on muscle metabolism; the IPA content was determined by metabonomics and ELISA. In an in vitro study, C2C12 cells were exposed to lipopolysaccharide (LPS) alone or LPS + IPA to verify the effect of IPA on muscle cell inflammation by transcriptome, and the involved mechanism was revealed by different functional assays. We observed that C. sporogenes colonization significantly increased the body weight and muscle weight gain, as well as the myogenic regulatory factors’ (MRFs) expression. In addition, C. sporogenes significantly improved host IPA content and decreased pro-inflammatory cytokine levels in the muscle tissue of mice. Subsequently, we confirmed that IPA promoted C2C12 cells’ proliferation by activating MRF signaling. IPA also effectively protected against LPS-induced C2C12 cells inflammation by activating Pregnane X Receptor and restoring the inhibited miR-26a-2-3p expression. miR-26a-2-3p serves as a novel muscle inflammation regulatory factor that could directly bind to the 3′-UTR of IL-1β, a key initiator factor in inflammation. The results suggested that C. sporogenes with its functional metabolite IPA not only helps muscle growth development, but also protects against inflammation, partly by the IPA/ miR-26a-2-3p /IL-1β cascade.
Single Extracellular Vesicle Analysis Performed by Imaging Flow Cytometry and Nanoparticle Tracking Analysis Evaluate the Accuracy of Urinary Extracellular Vesicle Preparation Techniques Differently
Authors: Droste Marvin , Tertel Tobias , Jeruschke Stefanie , Dittrich Robin , Kontopoulou Evangelia , Walkenfort Bernd , Börger Verena , Hoyer Peter F. , Büscher Anja K. , Thakur Basant K. , Giebel Bernd
Published: November 2021

Small extracellular vesicles isolated from urine (uEVs) are increasingly recognized as potential biomarkers. Meanwhile, different uEV preparation strategies exist. Conventionally, the performance of EV preparation methods is evaluated by single particle quantification, Western blot, and electron microscopy. Recently, we introduced imaging flow cytometry (IFCM) as a next-generation single EV analysis technology. Here, we analyzed uEV samples obtained with different preparation procedures using nanoparticle tracking analysis (NTA), semiquantitative Western blot, and IFCM. IFCM analyses demonstrated that urine contains a predominant CD9+ sEV population, which exceeds CD63+ and CD81+ sEV populations. Furthermore, we demonstrated that the storage temperature of urine samples negatively affects the recovery of CD9+ sEVs. Although overall reduced, the highest CD9+ sEV recovery was obtained from urine samples stored at −80 °C and the lowest from those stored at −20 °C. Upon comparing the yield of the different uEV preparations, incongruencies between NTA and IFCM data became apparent. Results obtained by both NTA and IFCM were consistent with Western blot analyses for EV marker proteins; however, NTA results correlated with the amount of the impurity marker uromodulin. Despite demonstrating that the combination of ultrafiltration and size exclusion chromatography appears as a reliable uEV preparation technique, our data challenge the soundness of traditional NTA for the evaluation of different EV preparation methods.
Doxorubicin-Resistant TNBC Cells Exhibit Rapid Growth with Cancer Stem Cell-like Properties and EMT Phenotype, Which Can Be Transferred to Parental Cells through Autocrine Signaling
Authors: Paramanantham Anjugam , Jung Eun , Kim Hye , Jeong Bae , Jung Jin-Myung , Kim Gon , Hong Soon , Lee Won Sup
Published: November 2021

Emerging evidence suggests that breast cancer stem cells (BCSCs), and epithelial–mesenchymal transition (EMT) may be involved in resistance to doxorubicin. However, it is unlear whether the doxorubicin-induced EMT and expansion of BCSCs is related to cancer dormancy, or outgrowing cancer cells with maintaining resistance to doxorubicin, or whether the phenotypes can be transferred to other doxorubicin-sensitive cells. Here, we characterized the phenotype of doxorubicin-resistant TNBC cells while monitoring the EMT process and expansion of CSCs during the establishment of doxorubicin-resistant MDA-MB-231 human breast cancer cells (DRM cells). In addition, we assessed the potential signaling associated with the EMT process and expansion of CSCs in doxorubicin-resistance of DRM cells. DRM cells exhibited morphological changes from spindle-shaped MDA-MB-231 cells into round-shaped giant cells. They exhibited highly proliferative, EMT, adhesive, and invasive phenotypes. Molecularly, they showed up-regulation of Cyclin D1, mesenchymal markers (β-catenin, and N-cadherin), MMP-2, MMP-9, ICAM-1 and down-regulation of E-cadherin. As the molecular mechanisms responsible for the resistance to doxorubicin, up-regulation of EGFR and its downstream signaling, were suggested. AKT and ERK1/2 expression were also increased in DRM cells with the advancement of resistance to doxorubicin. Furthermore, doxorubicin resistance of DRM cells can be transferred by autocrine signaling. In conclusion, DRM cells harbored EMT features with CSC properties possessing increased proliferation, invasion, migration, and adhesion ability. The doxorubicin resistance, and doxorubicin-induced EMT and CSC properties of DRM cells, can be transferred to parental cells through autocrine signaling. Lastly, this feature of DRM cells might be associated with the up-regulation of EGFR.
Evolution of the Early Spliceosomal Complex—From Constitutive to Regulated Splicing
Authors: Borao Sonia , Ayté José , Hümmer Stefan
Published: November 2021

Pre-mRNA splicing is a major process in the regulated expression of genes in eukaryotes, and alternative splicing is used to generate different proteins from the same coding gene. Splicing is a catalytic process that removes introns and ligates exons to create the RNA sequence that codifies the final protein. While this is achieved in an autocatalytic process in ancestral group II introns in prokaryotes, the spliceosome has evolved during eukaryogenesis to assist in this process and to finally provide the opportunity for intron-specific splicing. In the early stage of splicing, the RNA 5′ and 3′ splice sites must be brought within proximity to correctly assemble the active spliceosome and perform the excision and ligation reactions. The assembly of this first complex, termed E-complex, is currently the least understood process. We focused in this review on the formation of the E-complex and compared its composition and function in three different organisms. We highlight the common ancestral mechanisms in S. cerevisiae, S. pombe, and mammals and conclude with a unifying model for intron definition in constitutive and regulated co-transcriptional splicing.
Characterization and Genome Study of Novel Lytic Bacteriophages against Prevailing Saprophytic Bacterial Microflora of Minimally Processed Plant-Based Food Products
Authors: Wójcicki Michał , Średnicka Paulina , Błażejak Stanisław , Gientka Iwona , Kowalczyk Monika , Emanowicz Paulina , Świder Olga , Sokołowska Barbara , Juszczuk-Kubiak Edyta
Published: November 2021

The food industry is still searching for novel solutions to effectively ensure the microbiological safety of food, especially fresh and minimally processed food products. Nowadays, the use of bacteriophages as potential biological control agents in microbiological food safety and preservation is a promising strategy. The aim of the study was the isolation and comprehensive characterization of novel bacteriophages with lytic activity against saprophytic bacterial microflora of minimally processed plant-based food products, such as mixed leaf salads. From 43 phages isolated from municipal sewage, four phages, namely Enterobacter phage KKP 3263, Citrobacter phage KKP 3664, Enterobacter phage KKP 3262, and Serratia phage KKP 3264 have lytic activity against Enterobacter ludwigii KKP 3083, Citrobacter freundii KKP 3655, Enterobacter cloacae KKP 3082, and Serratia fonticola KKP 3084 bacterial strains, respectively. Transmission electron microscopy (TEM) and whole-genome sequencing (WGS) identified Enterobacter phage KKP 3263 as an Autographiviridae, and Citrobacter phage KKP 3664, Enterobacter phage KKP 3262, and Serratia phage KKP 3264 as members of the Myoviridae family. Genome sequencing revealed that these phages have linear double-stranded DNA (dsDNA) with sizes of 39,418 bp (KKP 3263), 61,608 bp (KKP 3664), 84,075 bp (KKP 3262), and 148,182 bp (KKP 3264). No antibiotic resistance genes, virulence factors, integrase, recombinase, or repressors, which are the main markers of lysogenic viruses, were annotated in phage genomes. Serratia phage KKP 3264 showed the greatest growth inhibition of Serratia fonticola KKP 3084 strain. The use of MOI 1.0 caused an almost 5-fold decrease in the value of the specific growth rate coefficient. The phages retained their lytic activity in a wide range of temperatures (from −20 °C to 50 °C) and active acidity values (pH from 4 to 11). All phages retained at least 70% of lytic activity at 60 °C. At 80 °C, no lytic activity against tested bacterial strains was observed. Serratia phage KKP 3264 was the most resistant to chemical factors, by maintaining high lytic activity across a broader range of pH from 3 to 11. The results indicated that these phages could be a potential biological control agent against saprophytic bacterial microflora of minimally processed plant-based food products.
The Impact of Social Media Activities on Brand Equity
Authors: Masa’deh Ra’ed , AL-Haddad Shafig , Al Abed Dana , Khalil Hadeel , AlMomani Lina , Khirfan Taghreed
Published: November 2021

This study aims to investigate the impact of Social Media Activities on brand equity (brand awareness and brand image). A cross-sectional quantitative study has been conducted using a validated questionnaire distributed to a convenience sample of 362 participants who used one or more forms of an Airline’s social media. Multiple Regression analysis was performed using SPSS version 20 to test the hypotheses. Results revealed a significant impact of Social Media Activities as a whole on brand equity. It was found that entertainment, customization, interaction and EWOM significantly affected the brand image, while customization, trendiness, interaction and EWOM significantly affected brand awareness. This study is one of few to examine the impact of social media activities on brand equity towards Airlines in Middle Eastern countries. The study provided several theoretical and practical implications that can benefit airline managers in their marketing efforts using various social media activities.
Research and Development of Metrological Assurance Elements for Leeb Hardness Measurements
Authors: Syasko Vladimir , Nikazov Artem
Published: November 2021

This article is devoted to contemporary topics of ensuring the uniformity of Leeb hardness measurements. The analysis of the physical principles of the Leeb hardness test is carried out, and the influence of the measuring transducer parameters on the measurement results is investigated. A four-level structure of the calibration hierarchy for the Leeb hardness scales is proposed, which ensures metrological traceability of hardness scales from the calibration reference machines (CRMs) to measuring instruments. A list of requirements for the 2nd grade CRM and the 2nd grade Leeb reference test blocks are formulated in accordance with the proposed calibration hierarchy draft and their values are calculated. A prototype was developed and a model of the 2nd grade Leeb hardness CRM and a set of Leeb reference test blocks were manufactured, as well as the compliance of their metrological characteristics with the requirements of the proposed calibration hierarchy draft and international standards was confirmed. The results of this work allow us to ensure the uniformity and reliability of the Leeb hardness measurements.
Prevention of Low Back Pain in Adults with a Back School-Based Intervention
Authors: Hernandez-Lucas Pablo , Lopez-Barreiro Juan , Garcia-Soidan Jose Luis , Romo-Perez Vicente
Published: November 2021

Background: Low back pain is highly prevalent and has a major socio-economic impact worldwide. Among the rehabilitation options is the Back School, which consists of programmes that include exercise and educational interventions to treat and prevent back pain. The effects of this type of programme are usually evaluated in patients with low back pain. The aim of this study was to evaluate the effects on low back functionality and the prevention of medical visits due to low back pain during one year of follow-up in a healthy adult population. Methods: A quasi-experimental study was conducted with 56 healthy participants who were divided into an experimental group (n = 30), who underwent the programme consisting of a total of 16 sessions, and a control group (n = 26), who did not undergo the intervention. All participants were administered the Partial Curl-up Test, Biering Sorense Test, Modified Schöber Test, and Toe Touch Test, and they completed the Short Form 36 Health Survey before and after the intervention. In addition, a telephone call was made to ask whether they attended a doctor for low back pain in the following year post-intervention. Results: In the experimental group, statistically significant improvements were observed in trunk muscle strength, spinal flexion joint range of motion, and hamstring flexibility, and they had fewer visits to the doctor for low back pain in the following year. Conclusions: The theoretical–practical programme based on the Back School seems to have beneficial effects on low back functionality by increasing its strength and flexibility in an adult population. In addition, this programme reduced the number of medical visits due to low back pain during the following year after the intervention.
Albumin-Bilirubin Score for Prediction of Outcomes in Heart Failure Patients Treated with Cardiac Resynchronization Therapy
Authors: Yamada Shinya , Kaneshiro Takashi , Yoshihisa Akiomi , Nodera Minoru , Amami Kazuaki , Nehashi Takeshi , Takeishi Yasuchika
Published: November 2021

Background: Liver function may be a useful indicator of response to cardiac resynchronization therapy (CRT). We aimed to investigate the clinical significance of albumin-bilirubin (ALBI) score, an assessment tool of liver function, on outcomes in heart failure (HF) patients treated with CRT. Methods: We studied 180 patients undergoing CRT. The ALBI score, derived from albumin and total bilirubin, and left ventricular ejection fraction (LVEF) were assessed before and 6 months after CRT. The patients were classified according to the ALBI score before CRT; High (>−2.60) or Low (≤−2.60) ALBI groups. The patients were then reclassified based on the ALBI score before and 6 months after CRT; High/High, High/Low, Low/High, and Low/Low ALBI groups. We evaluated the prognostic value of the ALBI score for HF deaths after CRT. Results: During a median follow-up period of 50 months, there were 41 (22.7%) HF deaths. A Cox proportional hazard analysis revealed that high ALBI scores at baseline were not related to HF deaths (hazard ratio, 1.907, p = 0.068). However, High/High ALBI scores, but not High/Low or Low/High ALBI scores, were an independent predictor of HF deaths compared with Low/Low ALBI scores (hazard ratio, 3.449, p = 0.008), implying that consistently high ALBI scores were associated with poor prognosis. The percentage change in LVEF from baseline to 6 months after CRT did not differ among the four groups, suggesting that left ventricular systolic function was not linked with the ALBI score. Conclusions: ALBI scores before and after CRT are a new indicator of CRT response, and have a predictive value for HF deaths in HF patients.
Potential Risk Factors for Isolated Hypothyroxinemia in Women of Childbearing Age—Results from Retrospective Analysis
Authors: Karbownik-Lewińska Małgorzata , Stępniak Jan , Lewiński Andrzej
Published: November 2021

Isolated hypothyroxinemia (IH) unfavorably affects reproduction. This study aimed to evaluate retrospectively if any routinely measured clinical/laboratory parameters are associated with IH among women of childbearing age hospitalized in the endocrine department. A group of 466 female non-pregnant inpatients (age range 13–57 years) was considered. IH (decreased free thyroxine (FT4) with normal TSH) was found in 8/466 patients (1.72%). Vitamin D deficiency (<30 ng/mL) was found in all patients with IH, whereas severe Vitamin D deficiency (<20 ng/mL) was found in 5/6. Vitamin D concentration was lower in IH females. FT4 concentration was lower in patients with severe vitamin D deficiency and correlated positively with vitamin D concentration. Insulin resistance index (IRI) was increased (>1.25) in 5/6 patients with IH. IRI was higher in IH patients and it was the only independent linear factor for IH in the univariate regression. FT4 concentration was lower in patients with increased IRI and correlated negatively with IRI. FT4 concentration correlated negatively with body mass index (BMI) and LDL cholesterol or triglycerides, and positively with HDL cholesterol or HDLC/cholesterol ratio. Vitamin D deficiency, insulin resistance and increased BMI (as potential causative factors), and abnormal lipid profile (as a possible consequence), are associated with IH in women of childbearing age. Eliminating risk factors for hypothyroxinemia may improve reproductive health.
Effect of Silica Based Nanoparticles against Plasmodium falciparum and Leishmania infantum parasites
Authors: Tsamesidis Ioannis , Lymperaki Evgenia , Egwu Chinedu O. , Pouroutzidou Georgia K. , Kazeli Konstantina , Reybier Karine , Bourgeade-Delmas Sandra , Valentin Alexis , Kontonasaki Eleana
Published: November 2021

Malaria and Leishmaniasis are two major parasitic diseases, endemic in large areas of tropical countries with high morbidity and mortality across the world. Nanoparticles in small sizes are specifically considered in medicine due to their ability to enter the cells, control the distribution of the administered drug and carry the drug specifically to the place of action. The present study aims to introduce the application of silica nanoparticles as new promising nanotools in malaria and leishmaniasis treatment. Ion doped silica nanomaterials revealed antileishmanial activities indicating the positive role of calcium, magnesium and copper to the surface of the particles against Leishmania parasites. Artemisinin-loaded nanoparticles presented the most promising antiparasitic properties with a sustained release able to overcome the parasite invasion. The sustainable release of artemisinin guarantee both the maintenance of its potential efficacy and also introduce an administration of drug to avoid subsequent drug resistance.