PHYSICAL AND CHEMICAL PROPERTIES OF BILEDURING THE ONSET AND MANIFESTATION OF CHOLANGITIS. ACUTE CHOLANGITIS: POSSIBLE TRIGGERING MECHANISMS
Authors:   V. T. Zaytsev Institute of General and Urgent Surgery of the National Academy of Medical Sciences of Ukraine, Boyko V.V. , Maloshtan A.V. ,   V. T. Zaytsev Institute of General and Urgent Surgery of the National Academy of Medical Sciences of Ukraine, Smachylo R.M. ,   V. T. Zaytsev Institute of General and Urgent Surgery of the National Academy of Medical Sciences of Ukraine, Nekliudov A.A. ,   V. T. Zaytsev Institute of General and Urgent Surgery of the National Academy of Medical Sciences of Ukraine, Tyshchenko A.M. ,   V. T. Zaytsev Institute of General and Urgent Surgery of the National Academy of Medical Sciences of Ukraine, Vovk V.A. ,   Regional Clinical Hospital, Klosova M.A. ,   V. T. Zaytsev Institute of General and Urgent Surgery of the National Academy of Medical Sciences of Ukraine, Volchenko O.V. ,   V. T. Zaytsev Institute of General and Urgent Surgery of the National Academy of Medical Sciences of Ukraine
Published: April 2021

Objective. To determine the possible triggering mechanism for the development and manifestation of acute suppurative cholangitis based on the study of the rheological, physical and chemical properties of bile. Methods. The bile of choledoch was studied inpatients (n=41) with calculous obstruction of the biliary tract, 25 of them had clinical picture of cholangitis. For comparison, 32 patients with asymptomatic choledocholithiasis were examined. Bile sampling was performed in each patient twice: the first bile intake was obtained during duodenoscopy and cannulation of the major papilla of the duodenum and endoscopic papillosphincterotomy and the second portion - during a follow-up examination three days after the endoscopic papillotomy. The biliary pH, viscosity, the amount of solids in it, the concentration of primary bile acids, microbial contamination and the intraductal pressure were studied. Results. For the first time, it has been hypothesized that a reduced concentration of bile acids in the bile causes a subsequent chain of triggering and manifestation of acute cholangitis. In the first bile intake the concentration of bile acids is three fold decreased in patients with acute cholangitis in comparison with the patients of control group, and in the second case it reliable increased but was lower than in the control group. It was a small amount of bile acids caused sedimentation of a colloidal bile solution, increased its viscosity and amount of dry sediment, caused precipitation and sludge. This chain process caused the instantaneous inclusion of concretion in sludge and acute obstruction of the choledoch. As a result of bacterial contamination, the microbial dissemination of bile increases by 10<sup>3</sup>-10<sup>4</sup> times, the choledoch becomes like an abscess. Endoscopic papillosphincterotomy breaks this chain and surgical management is the definitive mode of treatment for decompression and biliary track sanitation. Conclusion. A decrease of bile acids in the content of the bile causes a chain process of sedimentation, the formation of sludge and a complete block of the choledoch, which is the primary trigger for cholangitis. What this paper adds Scientific and theoretical principles which in their essencedetermine the possible mechanism of the development of cholangitis due to choledocholithiasis have been developed. The physicochemical properties of bile play a decisive role in the triggering mechanism of cholangitis. Reduction of the synthesis of bile acids in the liver or their resorption in the intestine starts an avalanche-like process of sedimentation in the micellar solution of bile in the choledoch, sludge with the stones appears, the pH decreases, intraductal pressure rises, as a result of bacterial infection microbial contamination the microbal dissemination increases by 10<sup>3</sup>-10<sup>4</sup> fold.