bluuebunny/crossref_metadata_2025 huggingface dataset:
The Institutional Structure of Land Use Planning for Urban Forest Protection in the Post-Socialist Transition Environment: Serbian Experiences
Authors: Maruna Marija , Crnčević Tijana , Milojević Milica P.
Published: July 2019

In recent decades, Serbia has been undergoing a period of post-socialist transition that has significantly altered the value system underlying spatial development due to alteration of ownership frameworks and land use rights. In consequence, issues have arisen of how to strike a balance between the various interests involved in the distribution of spatial resources and how to control the outcomes of public policies. Land use planning has been identified as an efficient instrument for implementing the public policy value framework. The objective of this paper is to identify the key points of land use planning in relation to urban forest management of significance for the maintenance of urban forests in the environment of post-socialist institutional transformation in Serbia. Seen as an institutional structure, the practice of land use planning in Serbia is the product of a stable interaction between the set of interrelated rules, procedures and organisational units that allows spatial development outcomes that take into account and safeguard land resources and, ultimately, urban forests. The research was carried out in relation to the concept of institutional transformation across three scales: macro/governance, meso/coordination and micro/agency: (a) components of the regulatory framework; (b) procedures for cooperation between stakeholders; and (c) specific activities of land use planning practice. As a result, the concept of Land use Planning for Urban Forest Protection (LUPUFP) in Serbia was established. It identifies components of institutional structure of importance for regulating system changes in the post-socialist transition environment and steering them towards the establishment of a value framework that allows the agenda of saving urban forests to be implemented.
Influence of Drought on Foliar Water Uptake Capacity of Temperate Tree Species
Authors: Schreel Jeroen D.M. , von der Crone Jonas S. , Kangur Ott , Steppe Kathy
Published: July 2019

Foliar water uptake (FWU) has been investigated in an increasing number of species from a variety of areas but has remained largely understudied in deciduous, temperate tree species from non-foggy regions. As leaf wetting events frequently occur in temperate regions, FWU might be more important than previously thought and should be investigated. As climate change progresses, the number of drought events is expected to increase, basically resulting in a decreasing number of leaf wetting events, which might make FWU a seemingly less important mechanism. However, the impact of drought on FWU might not be that unidirectional because drought will also cause a more negative tree water potential, which is expected to result in more FWU. It yet remains unclear whether drought results in a general increase or decrease in the amount of water absorbed by leaves. The main objectives of this study are, therefore: (i) to assess FWU-capacity in nine widely distributed key tree species from temperate regions, and (ii) to investigate the effect of drought on FWU in these species. Based on measurements of leaf and soil water potential and FWU-capacity, the effect of drought on FWU in temperate tree species was assessed. Eight out of nine temperate tree species were able to absorb water via their leaves. The amount of water absorbed by leaves and the response of this plant trait to drought were species-dependent, with a general increase in the amount of water absorbed as leaf water potential decreased. This relationship was less pronounced when using soil water potential as an independent variable. We were able to classify species according to their response in FWU to drought at the leaf level, but this classification changed when using drought at the soil level, and was driven by iso- and anisohydric behavior. FWU hence occurred in several key tree species from temperate regions, be it with some variability, which potentially allows these species to partly reduce the effects of drought stress. We recommend including this mechanism in future research regarding plant–water relations and to investigate the impact of different pathways used for FWU.
Seismic Site Classification from the Horizontal-to-Vertical Response Spectral Ratios: Use of the Spanish Strong-Motion Database
Authors: Pinzón Luis A. , Pujades Luis G. , Macau Albert , Carreño Emilio , Alcalde Juan M.
Published: July 2019

Normally, the average of the horizontal-to-vertical (H/V) ratios of the 5% damped response spectra of ground motions is used to classify the site of strong-motion stations. In these cases, only the three-orthogonal as-recorded acceleration components are used in the analysis, and all the vector compositions that can generate a different response for each period oscillator are excluded. In this study, the Spanish strong-motion database was used to classify the sites of accelerometric stations based on the predominant periods through the average horizontal-to-vertical spectral ratios (HVSR) of recorded ground motions. Moreover, the directionality effects using the vector composition of the horizontal components of ground motions were also considered in the estimations of H/V ratios. This consideration is a relevant novelty compared to the traditional H/V ratios methods. Only earthquakes with magnitudes above 3.5 and hypocentral distances below 200 km were selected, which resulted in 692 ground-motion records, corresponding to 86 stations, from events in the period between 1993 and 2017. After the analysis, a predominant-period site classification was assigned to each station. On the whole, the obtained mean and standard deviation values of the spectral ratios are comparable to those shown by other researchers. Therefore, the advantages of the proposed procedure, which takes the directionality effects into account, can be summarized as follows: (a) The obtained information is richer and gives enables more sophisticated and realistic analyses on the basis of percentiles and (b) it is easier to detect anomalous stations, sites, and/or accelerograms. Moreover, the method eliminates the effect of directionality as a contributor to epistemic uncertainty.
Cost–Benefit Analysis of Real-Time Influenza Testing for Patients in German Emergency Rooms
Authors: Diel Roland , Nienhaus Albert
Published: July 2019

Background: Seasonal influenza causes significant morbidity worldwide and has a substantial economic impact on the healthcare system. Objective: To assess the cost–benefit relation of implementing a real-time influenza test in emergency rooms (ER) of German hospitals. Methods: A deterministic decision-analytic model was developed simulating the incremental costs of using the Solana® Influenza A+B test, compared to those of using conventional clinical judgement alone to confirm or exclude influenza in adult ILI (influenza-like illness) patients, in German ER, prior to hospitalization. Direct costs were evaluated from the hospital perspective, considering resource use directly related to influenza testing and treatment, as well as indirect costs incurred by nosocomial influenza transmission. Results: Through base-case analysis and assuming an influenza prevalence of 42.6%, real-time testing with Solana® reduced average costs of hospitalized ILI patients by €132.61, per tested patient. Moreover, the Solana® saved €6.9 per tested patient in favor of the hospital. In probabilistic sensitivity analysis, under all reasonable assumptions, implementing the Solana® saved on average €144.13 as compared to applying the clinical-judgement-only strategy, thus, it was found to be constantly less expensive. Conclusions: Using highly sensitive and specific real-time influenza tests in ILI patients at German ER might significantly reduce hospital expenditures
The Interaction Effects of Burnout and Job Support on Peptic Ulcer Disease (PUD) among Firefighters and Policemen
Authors: Lin Ping-Yi , Wang Jong-Yi , Shih Dann-Pyng , Kuo Hsien-Wen , Liang Wen-Miin
Published: July 2019

Policemen and firefighters encounter numerous emergency events that frequently lead to high burnout and low job support, resulting in adverse health effects. A number of studies reported the correlation between job characteristics and the risk of peptic ulcer diseases (PUD) across various industries. However, there is very little research on evaluating the interaction effects of burnout and job support on the prevalence of PUD among firefighters and policemen. The objective of this study was to assess the interactional effects between burnout and job support on the prevalence of PUD among firefighters and policemen. This was a cross-sectional quantitative study. Registered, full-time police officers and firefighters in Taiwan were anonymously interviewed by a mail-delivered questionnaire. All female workers were excluded due to different job characteristics and a limited sample size. A total of 9328 firefighters and 42,798 policemen completed the questionnaire, with a response rate of 78.7%. Overall, prevalence rates of self-reported and self-reports of physician-diagnosed PUD were 8.3% and 6.5% for policemen and 7.1% and 5.5% for firefighters, respectively. There was a 22% reduced odds ratio of PUD as diagnosed by physicians for the group with low burnout and high job support, but an increased odds ratio of 53% for the group with high burnout and low job support, after adjusting for lifestyle and demographic variables. There must be an increase of job support and reduction of burnout through the modification of work structure and setting up of counseling services to reduce workplace stress and the prevalence of PUD among policemen and firefighters.
Addressing Food and Nutrition Security in Developed Countries
Authors: Pollard Christina Mary , Booth Sue
Published: July 2019

The guest editors of the special issue on Addressing Food and Nutrition Security in Developed Countries reflect on the 26 papers that were published as part of this issue and the scope of research contained therein. There is an extensive body of work, which focuses on topics ranging from the prevalence of food insecurity in developed countries, associations and determinants, measurement and monitoring, to reports of the lived experience and coping strategies of people who are living with food insecurity or and those who are a part of the charitable food sector. Very few solutions to address the problem of food insecurity in developed countries were offered, and many challenges highlighted. Further research is required to find the solutions to address the problem of food insecurity in developed countries, and important principles and values are proposed for those undertaking this work to embrace.
Oral Health-Related Quality of Life in Dutch Children Diagnosed with Oligodontia. A Cross-Sectional Study
Authors: Filius Marieke A.P. , Cune Marco S. , Créton Marijn , Vissink Arjan , Raghoebar Gerry M. , Visser Anita
Published: July 2019

There is need to get insight into condition-specific oral health-related quality of life in Dutch children with oligodontia. Between October 2014 and March 2017, 11–17-year-old oligodontia patients were approached to join a study assessing the impact of oligodontia on condition-specific oral health-related quality of life (OHrQoL). The patients received a condition-specific OHrQoL questionnaire prior to the start of orthodontic treatment. Non-oligodontia children in the same age group, but also requiring orthodontic treatment, were approached to serve as a control. The Fisher’s Exact Test was used for comparison purposes with the control group because of the small group sizes. Furthermore, subgroup analyses were performed for gender, age, number of congenitally missing teeth, tooth agenesis in the aesthetic region, orthodontic classification and microdontia via independent t-tests. p-values of <0.05 were considered statistically significant. Twenty-eight oligodontia patients and 23 controls agreed to participate. The oligodontia patients’ scores were comparable to the controls except for the items about dental appearance and treatment complexity. The impact of oligodontia on OHrQoL in youngsters is limited and mainly concerns dental appearance and the complexity of the treatment.
Patterns of Diet, Physical Activity, Sitting and Sleep Are Associated with Socio-Demographic, Behavioural, and Health-Risk Indicators in Adults
Authors: Oftedal Stina , Vandelanotte Corneel , Duncan Mitch J.
Published: July 2019

Our understanding of how multiple health-behaviours co-occur is in its infancy. This study aimed to: (1) identify patterns of physical activity, diet, sitting, and sleep; and (2) examine the association between sociodemographic and health-risk indicators. Pooled data from annual cross-sectional telephone surveys of Australian adults (2015–2017, n = 3374, 51.4% women) were used. Participants self-reported physical activity, diet, sitting-time, sleep/rest insufficiency, sociodemographic characteristics, smoking, alcohol use, height and weight to calculate body mass index (BMI), and mental distress frequency. Latent class analysis identified health-behaviour classes. Latent class regression determined the associations between health-behaviour patterns, sociodemographic, and health-risk indicators. Three latent classes were identified. Relative to a ‘moderate lifestyle’ pattern (men: 43.2%, women: 38.1%), a ‘poor lifestyle’ pattern (men: 19.9%, women: 30.5%) was associated with increased odds of a younger age, smoking, BMI ≥ 30.0 kg/m2, frequent mental distress (men and women), non-partnered status (men only), a lower Socioeconomic Index for Areas centile, primary/secondary education only, and BMI = 25.0–29.9 kg/m2 (women only). An ‘active poor sleeper’ pattern (men: 37.0%, women: 31.4%) was associated with increased odds of a younger age (men and women), working and frequent mental distress (women only), relative to a ‘moderate lifestyle’ pattern. Better understanding of how health-behaviour patterns influence future health status is needed. Targeted interventions jointly addressing these behaviours are a public health priority.
Health Needs Assessment of Five Pennsylvania Plain Populations
Authors: Miller Kirk , Yost Berwood , Abbott Christina , Thompson Buckland Scottie , Dlugi Emily , Adams Zachary , Rajagopalan Varun , Schulman Meryl , Hilfrank Kimberly , Cohen Mara A.
Published: July 2019

We performed a health needs assessment for five Plain communities in Pennsylvania from a random sample of households, comparing them to the general population of Pennsylvania adults. Plain respondents were more likely to drink well water, as likely to eat fruit and vegetables and much more likely to drink raw milk and be exposed to agricultural chemicals. Plain respondents were less likely to receive screening exams compared to the general population and there was variation from settlement to settlement in whether respondents had a regular doctor, whether they received preventive screenings or had their children vaccinated, with Mifflin County Amish generally lowest in these and Plain Mennonites highest. Plain respondents reported good physical and mental health compared to the general population but Groffdale Mennonite respondents had a high proportion of diagnoses of depression and were more likely to be receiving treatment for a mental health condition. Most Plain respondents would want a spouse tested for genetic disease with Mifflin County Amish least in favor of these tests. Despite their geographic and genetic isolation, the health of Plain communities in Pennsylvania is similar to that of other adults in the state.
Entrepreneurial Leadership and Turnover Intention of Employees: The Role of Affective Commitment and Person-job Fit
Authors: Yang Juan , Pu Bo , Guan Zhenzhong
Published: July 2019

The purpose of this study is to explore the causal system between entrepreneurial leadership and turnover intention of employees by examining the mediating effect of affective commitment and the moderating effect of person-job fit in start-ups. A quantitative approach was used to test the hypotheses and data were collected through the internet questionnaire tool. The authors selected employees from ventures newly established within the past five years and finally collected 427 questionnaires. The authors then used the hierarchical regression analysis method of Baron and Kenny for test mediating effect and the Hayes bootstrapping method for the test moderating effect by using Hayes’ SPSS PROCESS macro. The results demonstrated that affective commitment functions as a mediator and person-job fit functions as a moderator. This paper provides implications for start-up leaders that entrepreneurial leadership is an effective style of leadership and plays a crucial role which accompanies the development of venture.
Factors Associated with Burnout in Medical Academia: An Exploratory Analysis of Romanian and Moldavian Physicians
Authors: Popa-Velea Ovidiu , Diaconescu Liliana Veronica , Gheorghe Iuliana Raluca , Olariu Oana , Panaitiu Iolanda , Cerniţanu Mariana , Goma Ludmila , Nicov Irina , Spinei Larisa
Published: July 2019

This study aimed to assess the extent of burnout in Romanian and Moldavian academic physicians and to determine the predictive value of emotional intelligence (EI), coping strategies, work motivation (WM), perceived organizational support (POS), and the socio-demographic characteristics of burnout. Two hundred physicians (40% men, 60% women, mean age = 43.02, SD = 9.91) participated in the study. They were administered the Maslach Burnout Inventory−General Survey, Brief COPE Scale, Multidimensional Work Motivation Scale, Schutte’s Self-Report Emotional Intelligence Test, and Perceived Organizational Support Scale. Mann−Whitney U tests were used to assess the significance of intercountry differences, while hierarchical regressions were performed to investigate the predictive value of the independent variables on burnout. Moldavian participants had significantly lower scores in burnout and amotivation (p < 0.001) and higher scores in EI, POS, and WM (p < 0.001). The main burnout predictors were amotivation (β = 0.388, p < 0.001) and low POS (β = −0.313, p< 0.001) in Moldavian respondents, and WM (intrinsic: β = −0.620, p < 0.001; extrinsic: β = 0.406, p < 0.001) in Romanian participants. Moldavian respondents displayed better adjustment to academic stress. The distribution of burnout predictors suggests better sensitivity of respondents to organizational interventions in Moldova and to individual therapy in Romania. This data could serve to better tailor Public Health interventions addressing burnout in the academic environment.
The Function of Lipin in the Wing Development of Drosophila melanogaster
Authors: Duy Binh Tran , L. A. Pham Tuan , Nishihara Taisei , Thanh Men Tran , Kamei Kaeko
Published: July 2019

Lipin is evolutionarily conserved from yeast to mammals. Although its roles in lipid metabolism in adipocyte tissue, skeletal muscle, and the liver, and as a transcriptional co-activator are known, its functions during development are still under investigation. In this study, we analyzed the role of Drosophila lipin (dLipin) in development. Specifically, we showed that the tissue-selective knockdown of dLipin in the wing pouch led to an atrophied wing. Elevated DNA damage was observed in the wing imaginal disc of dLipin-knockdown flies. dLipin dysfunction induced accumulation of cells in S phase and significantly reduced the number of mitotic cells, indicating DNA damage-induced activation of the G2/M checkpoint. Reduced expression of cyclin B, which is critical for the G2 to M transition, was observed in the margin of the wing imaginal disc of dLipin-knockdown flies. The knockdown of dLipin led to increased apoptotic cell death in the wing imaginal disc. Thus, our results suggest that dLipin is involved in DNA replication during normal cell cycle progression in wing development of Drosophila melanogaster.
Lessons on Differential Neuronal-Death-Vulnerability from Familial Cases of Parkinson’s and Alzheimer’s Diseases
Authors: Franco Rafael , Navarro Gemma , Martínez-Pinilla Eva
Published: July 2019

The main risk of Alzheimer’s disease (AD) and Parkinson’s disease (PD), the two most common neurodegenerative pathologies, is aging. In contrast to sporadic cases, whose symptoms appear at >60 years of age, familial PD or familial AD affects younger individuals. Finding early biological markers of these diseases as well as efficacious treatments for both symptom relief and delaying disease progression are of paramount relevance. Familial early-onset PD/AD are due to genetic factors, sometimes a single mutation in a given gene. Both diseases have neuronal loss and abnormal accumulations of specific proteins in common, but in different brain regions. Despite shared features, the mechanisms underlying the pathophysiological processes are not known. This review aims at finding, among the genetic-associated cases of PD and AD, common trends that could be of interest to discover reliable biomarkers and efficacious therapies, especially those aimed at affording neuroprotection, i.e., the prevention of neuronal death.
The Lipid Status in Patients with Ulcerative Colitis: Sphingolipids are Disease-Dependent Regulated
Authors: Bazarganipour Sarah , Hausmann Johannes , Oertel Stephanie , El-Hindi Khadija , Brachtendorf Sebastian , Blumenstein Irina , Kubesch Alica , Sprinzl Kathrin , Birod Kerstin , Hahnefeld Lisa , Trautmann Sandra , Thomas Dominique , Herrmann Eva , Geisslinger Gerd , Schiffmann Susanne , Grösch Sabine
Published: July 2019

The factors that contribute to the development of ulcerative colitis (UC), are still not fully identified. Disruption of the colon barrier is one of the first events leading to invasion of bacteria and activation of the immune system. The colon barrier is strongly influenced by sphingolipids. Sphingolipids impact cell–cell contacts and function as second messengers. We collected blood and colon tissue samples from UC patients and healthy controls and investigated the sphingolipids and other lipids by LC-MS/MS or LC-QTOFMS. The expression of enzymes of the sphingolipid pathway were determined by RT-PCR and immunohistochemistry. In inflamed colon tissue, the de novo-synthesis of sphingolipids is reduced, whereas lactosylceramides are increased. Reduction of dihydroceramides was due to posttranslational inhibition rather than altered serine palmitoyl transferase or ceramide synthase expression in inflamed colon tissue. Furthermore, in human plasma from UC-patients, several sphinglipids change significantly in comparison to healthy controls. Beside sphingolipids free fatty acids, lysophosphatidylcholines and triglycerides changed significantly in the blood of colitis patients dependent on the disease severity. Our data indicate that detraction of the sphingolipid de novo synthesis in colon tissue might be an important trigger for UC. Several lipids changed significantly in the blood, which might be used as biomarkers for disease control; however, diet-related variabilities need to be considered.
Imaging Membrane Curvature inside a FcεRI-Centric Synapse in RBL-2H3 Cells Using TIRF Microscopy with Polarized Excitation
Authors: Machado Rosa , Bendesky Justin , Brown Madison , Spendier Kathrin , Hagen Guy M.
Published: July 2019

Total internal reflection fluorescence microscopy with polarized excitation (P-TIRF) can be used to image nanoscale curvature phenomena in live cells. We used P-TIRF to visualize rat basophilic leukemia cells (RBL-2H3 cells) primed with fluorescent anti-dinitrophenyl (anti-DNP) immunoglobulin E (IgE) coming into contact with a supported lipid bilayer containing mobile, monovalent DNP, modeling an immunological synapse. The spatial relationship of the IgE-bound high affinity IgE receptor (FcεRI) to the ratio image of P-polarized excitation and S-polarized excitation was analyzed. These studies help correlate the dynamics of cell surface molecules with the mechanical properties of the plasma membrane during synapse formation.
A Comparison Survey Study on RFID Based Anti-Counterfeiting Systems
Authors: Khalil Ghaith , Doss Robin , Chowdhury Morshed
Published: July 2019

Counterfeiting has always been a concern, costing a significant amount of money and causing losses in international trading markets. Radio frequency identification (RFID) tag Anti-counterfeiting is a conceptual solution that has received attention in the past few years. In this article, we present a survey study on the research topic of anti-counterfeiting products using RFID tags on merchandise. As this issue evolved in industry, there were several techniques used to address the problem; each technique uses a different concept and mechanism in resolving the issue. Each technique also has different pros and cons which we will address at the end of this paper with our findings. As we explore RFID technology and its implementation, we will discuss previous research before proceeding to the core of the topic of RFID Anti-counterfeiting based on the methods used. We compare the different techniques used at the end of the paper.
The Identity Construction of Migrants on Facebook
Authors: Mapelli Giovanna
Published: July 2019

Social network sites, such as Facebook, allow access to a series of resources or discursive forms that constitute a multimodal and dialogical system that transcends barriers of time and space, favouring transnational communication, something particularly important to migrants. In addition, the comments and dialogues that take place in such socialisation spaces allow us to develop a greater knowledge of the identity and positioning of the user with respect to others. With this work we analyse, from a qualitative point of view, 150 posts each containing at least five comments, published between 2017 and 2019, in each of five Facebook groups of Latin American migrants living in Italy: Uruguayans, Argentinians, Colombians, Peruvians and Venezuelans. We determine their role in the migratory process and how the digital environment affects the relationships between migrants. In addition, we investigate how the identities of migrants are negotiated and (re)defined in discursive practice. Results shows that social network sites are “transnational social spaces”, in which a community is based on bonds of solidarity that derive from a shared conception of collective identity, and they forge deterritorialised “community of feeling”.
Association between Circle of Willis Configuration and Rupture of Cerebral Aneurysms
Authors: Stojanović Nebojša N. , Kostić Aleksandar , Mitić Radisav , Berilažić Luka , Radisavljević Miša
Published: July 2019

Background and Objectives: Intracranial hemorrhage caused by the rupture of brain aneurysms occurs in almost 10 per 100,000 people whereas the incidence of such aneurysms is significantly higher, accounting for 4–9%. Linking certain factors to cerebral aneurysm rupture could help in explaining the significantly lower incidence of their rupture compared to their presence. The aim of this study is to determine the association between the corresponding circle of Willis configurations and rupture of cerebral aneurysms. Materials and Methods: A group of 114 patients treated operatively for aruptured cerebral aneurysm and a group of 56 autopsied subjects were involved in the study. Four basic types of the circle of Willis configurations were formed—two symmetric types A and C, and two asymmetric types B and D. Results: A statistically significantly higher presence of asymmetry of the circle of Willis was determined in the group of surgically-treated subjects (p = 0.001),witha significant presence of asymmetric Type B in this group (p < 0.001). The changeson the A1 segment in the group of surgically-treated subjects showed a statistically significant presence compared to the group of autopsied subjects (p = 0.001). Analyzing the presence of symmetry of the circle of Willis between the two groups, that is, the total presence of symmetric types A and C, indicated their statistically significant presence in the group of autopsied patients (p < 0.001). Conclusions: Changes such as hypoplasia or aplasia of A1 and the resulting asymmetry of the circle of Willis directly affect the possibility of the rupture of cerebral aneurysms. Detection of the corresponding types of the circle of Willis after diagnostic examination can be the basis for the development of a protocol for monitoring such patients.
Response of Phenylpropanoid Pathway and the Role of Polyphenols in Plants under Abiotic Stress
Authors: Sharma Anket , Shahzad Babar , Rehman Abdul , Bhardwaj Renu , Landi Marco , Zheng Bingsong
Published: July 2019

Phenolic compounds are an important class of plant secondary metabolites which play crucial physiological roles throughout the plant life cycle. Phenolics are produced under optimal and suboptimal conditions in plants and play key roles in developmental processes like cell division, hormonal regulation, photosynthetic activity, nutrient mineralization, and reproduction. Plants exhibit increased synthesis of polyphenols such as phenolic acids and flavonoids under abiotic stress conditions, which help the plant to cope with environmental constraints. Phenylpropanoid biosynthetic pathway is activated under abiotic stress conditions (drought, heavy metal, salinity, high/low temperature, and ultraviolet radiations) resulting in accumulation of various phenolic compounds which, among other roles, have the potential to scavenge harmful reactive oxygen species. Deepening the research focuses on the phenolic responses to abiotic stress is of great interest for the scientific community. In the present article, we discuss the biochemical and molecular mechanisms related to the activation of phenylpropanoid metabolism and we describe phenolic-mediated stress tolerance in plants. An attempt has been made to provide updated and brand-new information about the response of phenolics under a challenging environment.
The Cytotoxic Properties of Some Tricyclic 1,3-Dithiolium Flavonoids
Authors: Sarbu Laura G. , Shova Sergiu , Peptanariu Dragos , Sandu Isabela A. , Birsa Lucian M. , Bahrin Lucian G.
Published: July 2019

Background: Due to the emergence of multidrug resistant microorganisms, new classes of antibiotics are needed. In this paper, we present the cytotoxic effects of five tricyclic flavonoids, one of which was previously identified as a potent antimicrobial agent. Methods: All five derivatives were tested against human HOS and MCF7 cancer cell lines using a wound scratch assay. The cytotoxic properties of previously reported flavonoid 4a were also evaluated using the standard MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) and live/dead assays, using NHDF, HOS and MCF7 cell lines. Results: All five derivatives were found to inhibit to some degree the proliferation of cancer cells. 4a was also found to be less toxic towards regular versus cancerous human cells. Moreover, the minimum bactericidal concentration of 4a against Staphylococcus aureus was found to be non-toxic for any of the tested human cell lines. Conclusions: Derivative 4a has the potential of being used as a therapeutic agent against certain microorganisms. Further structure optimization is required for use against tumors.
Advanced Glycation End Products of Bovine Serum Albumin Suppressed Th1/Th2 Cytokine but Enhanced Monocyte IL-6 Gene Expression via MAPK-ERK and MyD88 Transduced NF-κB p50 Signaling Pathways
Authors: Shen Chieh-Yu , Wu Cheng-Han , Lu Cheng-Hsun , Kuo Yu-Min , Li Ko-Jen , Hsieh Song-Chou , Yu Chia-Li
Published: July 2019

Advanced glycation end products (AGE), the most known aging biomarker, may cause “inflamm-aging” (i.e., chronic low-grade inflammation that develops with aging) in both aged and diabetes groups. However, the molecular bases of inflamm-aging remain obscure. We prepared AGE by incubating BSA (0.0746 mmol/L) + glucose (0.5 mol/L) at 37 °C in 5% CO2–95% air for 1–180 days. The lysine glycation in BSA–AGE reached 77% on day 30 and 100% after day 130, whereas the glycation of arginine and cysteine was minimal. The Nε-(carboxymethyl)-lysine content in BSA–AGE was also increased with increasing number of incubation days. The lectin-binding assay revealed that the glycation of BSA not only altered the conformational structure, but lost binding capacity with various lectins. An immunological functional assay showed that BSA–AGE > 8 μg/mL significantly suppressed normal human Th1 (IL-2 and IFN-γ) and Th2 (IL-10) mRNA expression, whereas AGE > 0.5 μg/mL enhanced monocyte IL-6 production irrelevant to cell apoptosis. The AGE-enhanced monocyte IL-6 production was via MAPK–ERK and MyD88-transduced NF-κBp50 signaling pathways. To elucidate the structure–function relationship of BSA–AGE-enhanced IL-6 production, we pre-preincubated BSA–AGE with different carbohydrate-degrading, protein-degrading, and glycoprotein-degrading enzymes. We found that trypsin and carboxypeptidase Y suppressed whereas β-galactosidase enhanced monocyte IL-6 production. In conclusion, BSA–AGE exerted both immunosuppressive and pro-inflammatory effects that are the molecular basis of inflamm-aging in aged and diabetes groups.
Simplification of Carbon Bond Mechanism IV (CBM-IV) under Different Initial Conditions by Using Concentration Sensitivity Analysis
Authors: Cao Le , Li Simeng , Yi Ziwei , Gao Mengmeng
Published: July 2019

Carbon Bond Mechanism IV (CBM-IV) is a widely used reaction mechanism in which VOCs are grouped according to the molecular structure. In the present study, we applied a sensitivity analysis on the CBM-IV mechanism to clarify the importance of each reaction under two different initial conditions (urban and low-NOx scenarios). The reactions that exert minor influence on the reaction system are then screened out from the mechanism, so that a reduced version of the CBM-IV mechanism under specific initial conditions can be obtained. We found that in a typical urban condition, 11 reactions can be removed from the original CBM-IV mechanism, and the deviation is less than 5% between the results using the original CBM-IV mechanism and the reduced mechanism. Moreover, in a low-NOx initial condition, two more reactions, both of which are nitrogen-associated reactions, can be screened out from the reaction mechanism, while the accuracy of the simulation is still maintained. It is estimated that the reduction of the CBM-IV mechanism can save 11–14% of the computing time in the calculation of the chemistry in a box model simulation.
High Prevalence of Hypovitaminosis D in Institutionalized Elderly Individuals is Associated with Summer in a Region with High Ultraviolet Radiation Levels
Authors: Sousa Sara Estéfani S. , Sales Márcia Cristina , Araújo José Rodolfo T. , Sena-Evangelista Karine C.M. , Lima Kenio C. , Pedrosa Lucia F.C.
Published: July 2019

Vitamin D may play a significant role in regulating the rate of aging. The objective of the study was to assess vitamin D status and its associated factors in institutionalized elderly individuals. A total of 153 elderly individuals living in Nursing Homes (NH) were recruited into the study. Serum 25-hydroxyvitamin D [25(OH)D] concentration was used as the biomarker of vitamin D status, and it was considered as the dependent variable in the model. The independent variables were the type of NH, age-adjusted time of institutionalization, age, sex, skin color, body mass index, waist and calf circumference, physical activity practice, mobility, dietary intake of vitamin D and calcium, vitamin D supplementation, use of antiepileptics, and season of the year. Serum 25(OH)D concentrations less than or equal to 29 ng/mL were classified as insufficient vitamin D status. The prevalences of inadequate dietary intake of vitamin D and calcium were 95.4% and 79.7%, respectively. The prevalence of hypovitaminosis D was 71.2%, and the mean serum concentration of 25(OH)D was 23.9 ng/mL (95% confidence interval [CI]: 22.8–26.1). Serum 25(OH)D concentration was associated with the season of summer (p = 0.046). There were no associations with other independent variables (all p > 0.05). The present results showed that a high prevalence of hypovitaminosis D was significantly associated with summer in institutionalized elderly individuals.
How Satiating Are the ‘Satiety’ Peptides: A Problem of Pharmacology versus Physiology in the Development of Novel Foods for Regulation of Food Intake
Authors: Lim Jia Jiet , Poppitt Sally D.
Published: July 2019

Developing novel foods to suppress energy intake and promote negative energy balance and weight loss has been a long-term but commonly unsuccessful challenge. Targeting regulation of appetite is of interest to public health researchers and industry in the quest to develop ‘functional’ foods, but poor understanding of the underpinning mechanisms regulating food intake has hampered progress. The gastrointestinal (GI) or ‘satiety’ peptides including cholecystokinin (CCK), glucagon-like peptide 1 (GLP-1) and peptide YY (PYY) secreted following a meal, have long been purported as predictive biomarkers of appetite response, including food intake. Whilst peptide infusion drives a clear change in hunger/fullness and eating behaviour, inducing GI-peptide secretion through diet may not, possibly due to modest effects of single meals on peptide levels. We conducted a review of 70 dietary preload (DIET) and peptide infusion (INFUSION) studies in lean healthy adults that reported outcomes of CCK, GLP-1 and PYY. DIET studies were acute preload interventions. INFUSION studies showed that minimum increase required to suppress ad libitum energy intake for CCK, GLP-1 and PYY was 3.6-, 4.0- and 3.1-fold, respectively, achieved through DIET in only 29%, 0% and 8% of interventions. Whether circulating ‘thresholds’ of peptide concentration likely required for behavioural change can be achieved through diet is questionable. As yet, no individual or group of peptides can be measured in blood to reliably predict feelings of hunger and food intake. Developing foods that successfully target enhanced secretion of GI-origin ‘satiety’ peptides for weight loss remains a significant challenge.
Patients’, Pharmacy Staff Members’, and Pharmacy Researchers’ Perceptions of Central Elements in Prescription Encounters at the Pharmacy Counter
Authors: Kaae Susanne , Nørgaard Lotte Stig , Sporrong Sofia Kälvemark , Almarsdottir Anna Birna , Kofoed Mette , Daysh Rami Faris , Jowkar Nima
Published: July 2019

Background: Studies suggest that the way pharmacy counselling takes place does not fully support patients in obtaining optimal medicine use. To understand the basis of current challenges in pharmacy counselling, we investigated which selected related cues, i.e., objects, sounds, or circumstances in prescription encounters, patients, and pharmacy staff notice, and how they interpret these cues. Pharmacy practice researchers’ cue orientation was also investigated to explore possible differences to those of staff and patients. Methods: Twelve focus group interviews representing 5 community pharmacies (staff and patients) and 2 universities (researchers) were conducted during 2017–2018 in Denmark. A total of 20 patients, 22 pharmacy staff, and 6 pharmacy researchers participated. A theoretical analysis based on cue orientation and social appraisal was conducted. Results: Pharmacy staff, patients and researchers noticed different selected related cues in prescription encounters. Staff particularly noticed ‘types of patients’. Patients were more divided and grouped into three overall categories: ‘types of staff’, medical content, and the situation around the encounter. Pharmacy researchers noticed multiple cues. Different emotions were integrated in the construction of the cues. Conclusion: Differences in the cue orientation between all three groups were identified. The identified types of cues and emotions can explain an underlying dissatisfaction with the encounters. Patients lack, in particular, more personal contact. Staff need to consider these aspects to provide relevant counselling.
Transcriptional Regulation of Ascorbic Acid During Fruit Ripening in Pepper (Capsicum annuum) Varieties with Low and High Antioxidants Content
Authors: Chiaiese Pasquale , Corrado Giandomenico , Minutolo Maria , Barone Amalia , Errico Angela
Published: July 2019

Research on plant antioxidants, such as ascorbic acid (AsA) and polyphenols, is of increasing interest in plant science because of the health benefits and preventive role in chronic diseases of these natural compounds. Pepper (Capiscum annuum L.) is a major dietary source of antioxidants, especially AsA. Although considerable advance has been made, our understanding of AsA biosynthesis and its regulation in higher plants is not yet exhaustive. For instance, while it is accepted that AsA content in cells is regulated at different levels (e.g., transcriptional and post-transcriptional), their relative prominence is not fully understood. In this work, we identified and studied two pepper varieties with low and high levels of AsA to shed light on the transcriptional mechanisms that can account for the observed phenotypes. We quantified AsA and polyphenols in leaves and during fruit maturation, and concurrently, we analyzed the transcription of 14 genes involved in AsA biosynthesis, degradation, and recycling. The differential transcriptional analysis indicated that the higher expression of genes involved in AsA accumulation is a likely explanation for the observed differences in fruits. This was also supported by the identification of gene-metabolite relations, which deserve further investigation. Our results provide new insights into AsA differential accumulation in pepper varieties and highlight the phenotypic diversity in local germplasm, a knowledge that may ultimately contribute to the increased level of health-related phytochemicals.
Reinforcement of Polylactic Acid for Fused Deposition Modeling Process with Nano Particles Treated Bamboo Powder
Authors: Wang Cuicui , Smith Lee Miller , Zhang Wenfu , Li Mingpeng , Wang Ge , Shi Sheldon Q. , Cheng Haitao , Zhang Shuangbao
Published: July 2019

The focus of this report was to understand the tensile properties and dynamic mechanical properties of bamboo powder (BP) reinforced polylactic acid (PLA) composite filaments which were treated with nano calcium carbonate (CaCO3), cellulose nanofibers (CNF), and micro-crystalline cellulose (MCC) using impregnation modification technology. The storage modulus (E’) of nano CaCO3-BP/PLA, MCC-BP/PLA, and CNF-BP/PLA composite filaments increased compared with BP/PLA composite filaments before the glass transition temperature Tg. When the temperature was above Tg, the reinforcement effect of nano CaCO3, MCC, and CNF gradually became less apparent. The loss modulus (E’’) and loss factor (tan δmax) of the nano CaCO3-BP/PLA, MCC-BP/PLA, and CNF-BP/PLA composite filaments was higher than that of BP/PLA composite filaments produced by the “one-step” method. The tensile strength (TS) results showed a similar trend. Compared with the control samples, the TS of nano CaCO3-BP/PLA, MCC-BP/PLA, and CNF-BP/PLA composite filaments produced by the “one-step” method (and the “two-step” method) increased by 40.33% (and 10.10%), 32.35% (and −8.61%), and 12.32% (and −12.85%), respectively. The TS of nano CaCO3-BP/PLA, MCC-BP/PLA, and CNF-BP/PLA composite filaments produced by the “one-step” method was slightly higher than those produced by the “two-step” method. The elongation at break (EAB) of BP/PLA composite filaments was higher than that of BP/PLA samples treated with nano CaCO3, MCC, or CNF. The PLA and modified BP were readily accessible through a simple mixing process. The rheological investigation of such mixtures showed that nano CaCO3, CNF, and MCC have different effects on the processability and rheological properties of composites.
Aminomethylated Calix[4]resorcinarenes as Modifying Agents for Glycidyl Methacrylate (GMA) Rigid Copolymers Surface
Authors: Velásquez-Silva Betty Astrid , Castillo-Aguirre Alver , Rivera-Monroy Zuly Jenny , Maldonado Mauricio
Published: July 2019

Functionalization of tetrapropylcalix[4]resorcinarene, tetrapentylcalix[4]resorcinarene, tetranonylcalix[4]resorcinarene, and tetra-(4-hydroxyphenyl)calix[4]resorcinarene by means of aminomethylation reactions with the amino acids β-alanine and l-proline in the presence of aqueous formaldehyde was carried out. When β-alanine was used, the reaction products were tetrabenzoxazines. The reaction with tetra-(4-hydroxyphenyl)calix[4]resorcinarene did not proceed under the experimental conditions; therefore, l-proline was used, and the corresponding tetra-Mannich base was regio- and diasteroselectively formed. The products were characterized via FT-IR, 1H NMR, 13C NMR, and elemental analysis. With these aminomethylated-calix[4]resorcinarenes, the chemical surface modification of the copolymers poly(GMA–co–EDMA) and poly(BMA–co–EDMA–co–MMA) in a basic medium was studied. The results were quite satisfactory, obtaining the corresponding copolymers functionalized by nucleophilic substitution reaction and ring-opening between the carboxyl group of the upper rim of aliphatic calix[4]resorcinarenes and the hydroxyl group of the lower rim in the aromatic calix[4]resorcinarene and the epoxy group of the glycidyl methacrylate residue of each copolymer. The modified copolymers were characterized via FT-IR, scanning electron microscopy imaging, and elemental analysis. Finally, the modified copolymer surfaces exhibited interaction with peptides, showing their potential application in chromatographic separation techniques such as high-performance liquid chromatography.
A2RMNet: Adaptively Aspect Ratio Multi-Scale Network for Object Detection in Remote Sensing Images
Authors: Qiu Heqian , Li Hongliang , Wu Qingbo , Meng Fanman , Ngan King Ngi , Shi Hengcan
Published: July 2019

Object detection is a significant and challenging problem in the study area of remote sensing and image analysis. However, most existing methods are easy to miss or incorrectly locate objects due to the various sizes and aspect ratios of objects. In this paper, we propose a novel end-to-end Adaptively Aspect Ratio Multi-Scale Network (A 2 RMNet) to solve this problem. On the one hand, we design a multi-scale feature gate fusion network to adaptively integrate the multi-scale features of objects. This network is composed of gate fusion modules, refine blocks and region proposal networks. On the other hand, an aspect ratio attention network is leveraged to preserve the aspect ratios of objects, which alleviates the excessive shape distortions of objects caused by aspect ratio changes during training. Experiments show that the proposed A 2 RMNet significantly outperforms the previous state of the arts on the DOTA dataset, NWPU VHR-10 dataset, RSOD dataset and UCAS-AOD dataset by 5.73 % , 7.06 % , 3.27 % and 2.24 % , respectively.
Instrumented Crutch Tip for Monitoring Force and Crutch Pitch Angle
Authors: Sesar Iñigo , Zubizarreta Asier , Cabanes Itziar , Portillo Eva , Torres-Unda Jon , Rodriguez-Larrad Ana
Published: July 2019

In rehabilitation procedures related to the lower limbs, gait monitoring is an important source of information for the therapist. However, many of the approaches proposed in the literature require the use of uncomfortable and invasive devices. In this work, an instrumented tip is developed and detailed, which can be connected to any crutch. The instrumented tip provides objective data of the crutch motion, which, combined with patient movement data, might be used to monitor the daily activities or assess the recovery status of the patient. For that purpose, the tip integrates a two-axis inclinometer, a tri-axial gyroscope, and a force sensor to measure the force exerted on the crutch. In addition, a novel algorithm to estimate the pitch angle of the crutch is developed. The proposed approach is tested experimentally, obtaining acceptable accuracies and demonstrating the validity of the proposed lightweight, portable solution for gait monitoring.