bluuebunny/crossref_metadata_2025 huggingface dataset:
Multimodality OCT, IVUS and FFR evaluation of coronary intermediate grade lesions in women vs. men
Authors: Baruś Piotr , Piasecki Adam , Gumiężna Karolina , Bednarek Adrian , Dunaj Piotr , Głód Marcin , Sadowski Karol , Ochijewicz Dorota , Rdzanek Adam , Pietrasik Arkadiusz , Grabowski Marcin , Kochman Janusz , Tomaniak Mariusz
Published: June 2023

Background

The pathophysiology of atherosclerotic plaque formation and its vulnerability seem to differ between genders due to contrasting risk profiles and sex hormones, however this process is still insufficiently understood. The aim of the study was to compare the differences between sexes regarding the optical coherence tomography (OCT), intravascular ultrasound (IVUS) and fractional flow reserve (FFR)-derived coronary plaque indices.

Methods

In this single-center multimodality imaging study patients with intermediate grade coronary stenoses identified in coronary angiogram (CAG) were evaluated using OCT, IVUS and FFR. Stenoses were considered significant when the FFR value was ≤0.8. Minimal lumen area (MLA), was analyzed by OCT in addition to plaque stratification into fibrotic, calcific, lipidic and thin-cap fibroatheroma (TCFA). IVUS was used for evaluation of lumen-, plaque- and vessel volume, as well as plaque burden.

Results

A total of 112 patients (88 men and 24 women) with chronic coronary syndromes (CCS), who underwent CAG were enrolled. No significant differences in baseline characteristics were present between the study groups. The mean FFR was 0.76 (0.73–0.86) in women and 0.78 ± 0.12 in men (p = 0.695). OCT evaluation showed a higher prevalence of calcific plaques among women than men p = 0.002 whereas lipid plaques were more frequent in men (p = 0.04). No significant differences regarding minimal lumen diameter and minimal lumen area were found between the sexes. In IVUS analysis women presented with significantly smaller vessel area, plaque area, plaque volume, vessel volume (11.1 ± 3.3 mm2 vs. 15.0 ± 4.6 mm2p = 0.001, 6.04 ± 1.7 mm2 vs. 9.24 ± 2.89 mm2p < 0.001, 59.8 ± 35.2 mm3 vs. 96.3 (52.5–159.1) mm3p = 0.005, 106.9 ± 59.8 mm3 vs. 153.3 (103–253.4) mm3p = 0.015 respectively). At MLA site plaque burden was significantly greater for men than women (61.50 ± 7.7% vs. 55.5 ± 8.0% p = 0.005). Survival did not differ significantly between women and men (94.6 ± 41.9 months and 103.51 ± 36.7 months respectively; p = 0.187).

Conclusion

The presented study did not demonstrate significant differences in FFR values between women and men, yet a higher prevalence of calcific plaques by OCT and lower plaque burden at the MLA site by IVUS was found in women vs. men.
A deep-learning approach for myocardial fibrosis detection in early contrast-enhanced cardiac CT images
Authors: Penso Marco , Babbaro Mario , Moccia Sara , Baggiano Andrea , Carerj Maria Ludovica , Guglielmo Marco , Fusini Laura , Mushtaq Saima , Andreini Daniele , Pepi Mauro , Pontone Gianluca , Caiani Enrico G.
Published: June 2023

Aims

Diagnosis of myocardial fibrosis is commonly performed with late gadolinium contrast-enhanced (CE) cardiac magnetic resonance (CMR), which might be contraindicated or unavailable. Coronary computed tomography (CCT) is emerging as an alternative to CMR. We sought to evaluate whether a deep learning (DL) model could allow identification of myocardial fibrosis from routine early CE-CCT images.

Methods and results

Fifty consecutive patients with known left ventricular (LV) dysfunction (LVD) underwent both CE-CMR and (early and late) CE-CCT. According to the CE-CMR patterns, patients were classified as ischemic (n = 15, 30%) or non-ischemic (n = 35, 70%) LVD. Delayed enhancement regions were manually traced on late CE-CCT using CE-CMR as reference. On early CE-CCT images, the myocardial sectors were extracted according to AHA 16-segment model and labeled as with scar or not, based on the late CE-CCT manual tracing. A DL model was developed to classify each segment. A total of 44,187 LV segments were analyzed, resulting in accuracy of 71% and area under the ROC curve of 76% (95% CI: 72%−81%), while, with the bull’s eye segmental comparison of CE-CMR and respective early CE-CCT findings, an 89% agreement was achieved.

Conclusions

DL on early CE-CCT acquisition may allow detection of LV sectors affected with myocardial fibrosis, thus without additional contrast-agent administration or radiational dose. Such tool might reduce the user interaction and visual inspection with benefit in both efforts and time.
Mastering the learning curve of endoscopic mitral valve surgery
Authors: Aerts Luca , Sardari Nia Peyman
Published: June 2023

Endoscopic mitral valve surgery is a challenging procedure. Surgical volume is mandatory to achieve sufficient proficiency and superior results. To this date the learning curve has proven to be challenging. Offering high-fidelity simulation based training for both residents as experienced surgeons can help in establishing and enlarging surgical competences in shorter time without intraoperative trial and error.
Case Report: do heart transplant candidates benefit from mechanically supported revascularization?
Authors: Pyka Lukasz , Szkodzinski Janusz , Piegza Jacek , Swietlińska Malgorzata , Gąsior Mariusz
Published: June 2023

Introduction

Recently published studies suggest that percutaneous coronary intervention (PCI) has no significant impact on outcomes in patients with heart failure and stable coronary artery disease. The use of percutaneous mechanical circulatory support is growing, but its value is still uncertain. If large areas of viable myocardium are ischemic, the benefit from revascularization should be evident. In such instances, we should strive for complete revascularization. The use of mechanical circulatory support in such cases is vital because it provides hemodynamic stability throughout the complex procedure.

Case report

We present a case of a 53-year-old male heart transplant candidate with type 1 diabetes mellitus, initially considered unsuitable for revascularization and qualified for heart transplantation, transferred to our center due to acute decompensated heart failure. At this time, the patient had temporary contraindications for heart transplantation. As the patient was considered no-option, we have decided to reassess the possibility of revascularization. The heart team opted for a high-risk mechanically supported PCI with the aim of complete revascularization. A complex multivessel PCI was performed with optimal effect. The patient was weaned off dobutamine on the second day post-PCI. Four months post-discharge, he remains stable, is in NYHA II class, and has no chest pain. Control echocardiography showed improved ejection fraction. The patient is not a heart transplant candidate anymore.

Conclusions

This case report shows that we must strive for revascularization in select heart failure cases. The outcome of this patient suggests that heart transplant candidates with potentially viable myocardium should be considered for revascularization, especially as the shortage of donors persists. In the most complex coronary anatomy and severe heart failure, mechanical support in the procedure might be essential.
Painful and recurring injection site reaction to alirocumab and evolocumab in a young woman with familial hypercholesterolemia and effective therapeutic alternative based on inclisiran: a case report
Authors: Allevi Massimiliano , Sarnari Silvia , Giulietti Federico , Spannella Francesco , Di Pentima Chiara , Sarzani Riccardo
Published: June 2023

A 28-year-old woman with autosomal dominant familial hypercholesterolemia (FH) with a probable coexistent polygenic contribution causing very high low-density lipoprotein-cholesterol (LDL-C) levels, started therapy with the proprotein convertase subtilisin/kexin type 9-inhibitor (PCSK9i) alirocumab, in addition to high-intensity statin plus ezetimibe. Forty-eight hours after the second injection of alirocumab, the patient developed a painful palpable injection site reaction (ISR) that recurred after the third administration of the drug. Treatment was then switched to evolocumab, another PCSK9i, but the patient had an ISR with similar features. The most conceivable cause of the ISR was a cell-mediated hypersensitivity reaction to polysorbate, an excipient contained in both drugs. Although ISR after PCSK9i administration is usually transient and does not compromise the continuation of treatment, in this case the recurrence of such side effect in an exacerbated way led to treatment withdrawal, with a subsequent re-exposure to increased cardiovascular (CV) risk. As soon as it became available in clinical practice, the patient started treatment with inclisiran, a small interfering RNA targeting hepatic PCSK9 synthesis. No adverse events were reported after inclisiran administration and LDL-C levels decreased significantly, confirming the evidence that this innovative approach to hypercholesterolemia is a safe and effective resource in patients at high CV risk who cannot achieve LDL-C goal with conventional lipid-lowering therapies and antibody-based PCSK9i.
Application of next generation sequencing in cardiology: current and future precision medicine implications
Authors: Papadopoulou Eirini , Bouzarelou Dimitra , Tsaousis George , Papathanasiou Athanasios , Vogiatzi Georgia , Vlachopoulos Charalambos , Miliou Antigoni , Papachristou Panagiota , Prappa Efstathia , Servos Georgios , Ritsatos Konstantinos , Seretis Aristeidis , Frogoudaki Alexandra , Nasioulas George
Published: June 2023

Inherited cardiovascular diseases are highly heterogeneous conditions with multiple genetic loci involved. The application of advanced molecular tools, such as Next Generation Sequencing, has facilitated the genetic analysis of these disorders. Accurate analysis and variant identification are required to maximize the quality of the sequencing data. Therefore, the application of NGS for clinical purposes should be limited to laboratories with a high level of technological expertise and resources. In addition, appropriate gene selection and variant interpretation can result in the highest possible diagnostic yield. Implementation of genetics in cardiology is imperative for the accurate diagnosis, prognosis and management of several inherited disorders and could eventually lead to the realization of precision medicine in this field. However, genetic testing should also be accompanied by an appropriate genetic counseling procedure that clarifies the significance of the genetic analysis results for the proband and his family. In this regard, a multidisciplinary collaboration among physicians, geneticists, and bioinformaticians is imperative. In the present review, we address the current state of knowledge regarding genetic analysis strategies employed in the field of cardiogenetics. Variant interpretation and reporting guidelines are explored. Additionally, gene selection procedures are accessed, with a particular emphasis on information concerning gene-disease associations collected from international alliances such as the Gene Curation Coalition (GenCC). In this context, a novel approach to gene categorization is proposed. Moreover, a sub-analysis is conducted on the 1,502,769 variation records with submitted interpretations in the Clinical Variation (ClinVar) database, focusing on cardiology-related genes. Finally, the most recent information on genetic analysis's clinical utility is reviewed.
Experimental study on the shear failure of layered rock bridges
Authors: Yu Jianxin , Zhou Zhibin , Liu Huanchun , Zhou Lianhao , Li Zhenzhen , Cheng Long
Published: June 2023

The structural face of rock plays a decisive role in the stability of geotechnical engineering, and the mechanism of failure destabilization of layered rock masses is still unclear. By preparing rock-like materials, shear failure tests on layered rock masses were carried out under different bedding dip angles, different rock bridge width, different normal forces, and forward and reverse shear effects. With the increase in the rock bridge width, the influence of the joint inclination angle on the shear failure process of the rock sample decreases; the peak shear strength of the specimen is the largest when the joint inclination angle is 60°, followed by 90°, 30° and 0° from large to small; the failure mode in forward shear is mainly tensile failure, and the failure mode in reversed shear is mainly shear failure; the peak number of acoustic emission events is proportional to the joint inclination angle and the rock bridge width of the specimen, and the number of acoustic emission events is proportional to the joint inclination angle and the rock bridge width of the specimen. The number of acoustic emission peak events is proportional to the bedding angle of the sample and the width of the rock bridge. The peak number of acoustic emission events in the reverse shear of the layered rock mass is greater than that in the forward shear. The angle of the lamina and the width of the rock bridge are both important factors affecting the strength of the rock. The results of this study provide a basis for identifying the shear failure mechanism of rock bridge in layered rock masses.
The return to university after fieldwork: toward disrupting practice-theory challenges identified by mathematics teacher educators
Authors: Bjerke Annette Hessen , Nolan Kathleen
Published: June 2023

In this paper, we present on a research study that was framed in disruptive pedagogy (DP) to examine the post-field context of mathematics teacher educators’ (MTEs’) practices. We open by referring to common challenges discussed in the literature related to theory-practice transitions of prospective teachers (PTs) as they move from university courses to their field placement. After reviewing these challenges, we then shift our focus toward understanding what MTEs see as challenges in the post-field context of teacher education programs; that is, what practice-theory challenges are identified by MTEs as PTs make the transition from field back to university. Briefly, our thematic analysis suggests that, in the post-field context of teacher education programs, MTEs are challenged by organizational issues and institutional structures; by PTs’ return to the university armed with superficial placement stories and unexamined indicators of “good mathematics teaching”; and by the significant emphasis PTs place on mentor voices and ways of teaching, often including unfavorable views on the value of reform teaching. Simply put, MTEs expressed being challenged by PTs’ skepticism, resistance, and lack of conviction toward the role of the university. Additionally, MTEs reported being challenged by their own feelings of resignation that our analysis suggests stems from a growing list of challenges which can result in some MTEs stepping down and settling on a pragmatic approach to their post-field mathematics teaching. To close, implications for MTEs are discussed by pointing specifically to the potential of DP for unpacking practice-theory transitions and considering the creation of a post-field third space.
Analysis of eye movements to study drawing in the context of vector fields
Authors: Hahn Larissa , Klein Pascal
Published: June 2023

Research has shown that visual representations can substantially enhance the learning and understanding of STEM concepts; despite this, students tend to struggle in using them fluently and consistently. Consequently, educators advocate for explicit instructions that support the coordination of multiple representations, especially when concepts become more abstract and complex. For recent years, the drawing (or sketching) technique has received increasing attention. Theoretical considerations and prior research suggest that drawing has the potential to support knowledge construction and to provide cognitive relief. In this article, we present two studies that investigate the impact of drawing activities in a multi-representational, instruction-based learning scenario from physics, more precisely, in the context of vector fields. Further, mobile and remote eye tracking was used to record students' gaze behavior in addition to monitoring indicators of performance and cognitive load. Here, eye movements provide information about cognitive processes during the completion of the instruction, on the one hand, and during subsequent problem solving, on the other hand. Comparisons of a treatment group instructed with drawing activities and a control group instructed without drawing activities revealed significant differences in students' perceived cognitive load (p = 0.02, d = 0.47 and p = 0.0045, d = 0.37), as well as their response accuracy (p = 0.02, d = 0.51) and their response confidence (p = 0.02, d = 0.55 and p = 0.004, d = 0.64) during assessment after instruction (N = 84). Moreover, students instructed with drawing activities were found to distribute more visual attention to important parts of the instruction (vector field diagram and instructional text, N = 32) compared to the control group and, further, showed effective, expert-like behaviors during subsequent problem solving (N = 53). Finally, as a contribution to current trends in eye-tracking research, the application of mobile and remote eye-tracking in drawing-based learning and assessment scenarios is compared and critically discussed.
Cultivating critical consciousness through a Global Health Book Club
Authors: Collins Sarah L. , Case Stuart J. , Rodriguez Alexandra K. , Allen Acquel C. , Wood Elizabeth A.
Published: June 2023

Introduction

Central to public health practice is mindfulness and intentionality toward achieving social justice and health equity. However, there is limited literature published on how educators are integrating these concepts into their curricular, pedagogical and instructional efforts. The goal of this study was to leverage the pluralistic views, social identities, and demographics within the classroom to explore the effects of introducing a Global Health Book Club (GHBC) assignment focused on identity of culture, equity, and power. We also sought to explore the use of first-account narratives illustrating the human experience as an instructional strategy to cultivate an empathic understanding of global health threats, while fostering critical consciousness toward one’s positionality within macro-level contexts. Finally, students were encouraged to reflect on their lived cultural experiences and engage in open and authentic dialogue with their peers.

Methods

We implemented a four-week GHBC assignment within an undergraduate global public health course. At the conclusion of the GHBC, students engaged in a reflective Individual Analysis Paper, which captured students’ perspectives on their cultural values and traditions, how these views shaped their understanding of their book, and evaluate whether their global perspective had changed as a result of the assignment. Thirty-one students consented to have their Individual Analysis Paper downloaded and de-identified for analysis. Student responses were analyzed using inductive thematic analysis procedures.

Results

Through our analysis, six themes, with several coinciding categories, were identified as salient. The themes include Demonstrated Empathy, Personal Reflection and Growth, Personally Inspired, Immersive Learning Experience, Broadened Perspective, and Provoked Emotion.

Discussion

Our findings support that a GHBC assignment is a viable and effective mechanism for engaging students in critical reflection, critical motivation and critical action. In cultivating a learning environment that promotes student-centered learning and active participation, students exemplified agency in their own learning. This work can serve as an exemplary model for other public health educators to engage students in reflective-based assignments regarding their positionality and critical consciousness. By utilizing frameworks conceived out of antiracism, diversity, equity, and inclusion, our work presents an innovative activity in engaging students in decolonization efforts within global public health practice.
The influence of self-esteem, depression, and life satisfaction on the future expectations of Peruvian university students
Authors: Mamani-Benito Oscar , Carranza Esteban Renzo Felipe , Caycho-Rodríguez Tomás , Castillo-Blanco Ronald , Tito-Betancur Madona , Alfaro Vásquez Rosa , Ruiz Mamani Percy G.
Published: June 2023

Introduction

Strengthening the self-confidence of future professionals can help them achieve professional goals with a likelihood of being able to achieve them in the future. The objective was to determine whether self-esteem, depression, and life satisfaction influence the future expectations of Peruvian university students.

Methods

A total of 708 university students participated (64.8% were female, mean age = 21.49; SD = 4.83). The Rosenberg Self-Esteem Scale, the Patient Health Questionnaire-2, the Life Satisfaction Scale, and the Adolescent Future Expectations Scale were used. Structural equation modeling was employed to test the impact of selfesteem, depression, and life satisfaction on future expectations.

Results

The model relating the study variables demonstrated an adequate fit (p < 0.001, CFI = 0.940, RMSEA = 0.073), confirming the relationship between self-esteem (β = 0.33, p < 0.001), life satisfaction (β = 0.28, p < 0.001), and depression (β =  −0.14, p < 0.010.) with future expectations. This model explained 36% of future expectations.

Discussion

In conclusion, future expectations may be affected by low levels of self-esteem and life satisfaction and high levels of depression.
Transiently impaired endothelial function during thyroid hormone withdrawal in differentiated thyroid cancer patients
Authors: Hou Li-ying , Li Xiao , Zhang Guo-qiang , Xi Chuang , Shen Chen-tian , Song Hong-jun , Bai Wen-kun , Qiu Zhong-ling , Luo Quan-yong
Published: June 2023

Purpose

Endothelial dysfunction, which was associated with chronic hypothyroidism, was an early event in atherosclerosis. Whether short-term hypothyroidism following thyroxine withdrawal during radioiodine (RAI) therapy was associated with endothelial dysfunction in patients with differentiated thyroid cancer (DTC) was unclear. Aim of the study was to assess whether short-term hypothyroidism could impair endothelial function and the accompanied metabolic changes in the whole process of RAI therapy.

Methods

We recruited fifty-one patients who underwent total thyroidectomy surgery and would accept RAI therapy for DTC. We analyzed thyroid function, endothelial function and serum lipids levels of the patients at three time points: the day before thyroxine withdrawal(P1), the day before 131I administration(P2) and 4-6 weeks after RAI therapy(P3). A high-resolution ultrasound named flow-mediated dilation (FMD) was used to measure endothelial function of the patients.

Results

We analyzed the changes of FMD, thyroid function and lipids at three time points. FMD(P2) decreased significantly compared to FMD(P1) (P1vsP2, 8.05 ± 1.55vs 7.26 ± 1.50, p<0.001). There was no significant difference between FMD(P3) and FMD(P1) after restoring TSH (thyroid stimulating hormone) suppression therapy (P1 vs P3, 8.05 ± 1.55 vs 7.79 ± 1.38, p=0.146). Among all parameters, the change of low-density lipoprotein (ΔLDL) was the only factor correlated negatively with the change of FMD (ΔFMD) throughout the RAI therapy process (P1-2, r=-0.326, p=0.020; P2-3, r=-0.306, p=0.029).

Conclusion

Endothelial function was transiently impaired in DTC patients at short-term hypothyroidism state during the RAI therapy, and immediately returned to the initial state after restoring TSH suppression therapy.
Tocilizumab improves clinical outcome in patients with active corticosteroid-resistant moderate-to-severe Graves’ orbitopathy: an observational study
Authors: Boutzios Georgios , Chatzi Sofia , Goules Andreas V. , Mina Areti , Charonis George C. , Vlachoyiannopoulos Panayiotis G. , Tzioufas Athanasios G.
Published: June 2023

Background

Graves’ orbitopathy (GO) is an autoimmune disorder affecting the orbital fat and muscles. A significant role of IL-6 in the pathogenesis of GO has been described and tocilizumab (TCZ), an IL-6 inhibitor targeting IL-6R has been given in some patients. The aim of our case study was to evaluate the therapeutic outcome of TCZ in non-responders to first line treatments with corticosteroids.

Methods

We conducted an observational study of patients with moderate to severe GO. Twelve patients received TCZ in intravenous infusions at a dose of 8mg/kg every 28 days for 4 months and followed up for additionally 6 weeks. The primary outcome was improvement in CAS by at least 2 points, 6 weeks after the last dose of TCZ. Secondary outcomes included CAS <3 (inactive disease) 6 weeks after TCZ last dose, reduced TSI levels, proptosis reduction by > 2mm and diplopia response.

Results

The primary outcome, was achieved in all patients 6 weeks after treatment course. Furthermore all patients had inactive disease 6 weeks after treatment cessation. Treatment with TCZ reduced significantly median CAS by 3 units (p=0.002), TSI levels by 11.02 IU/L (p=0.006), Hertel score on the right eye by 2.3 mm (p=0.003), Hertel score on the left eye by 1.6 mm (p=0.002), while diplopia persisted in fewer patients (25%) after treatment with TCZ (not statistically significant, p=0.250). After treatment with TCZ, there was a radiological improvement in 75% of patients, while 16.7% showed no response, and in 8.3% of patients deterioration was established.

Conclusion

Tocilizumab appears to be a safe and cost effective therapeutic option for patients with active, corticosteroid-resistant, moderate to severe Graves’ orbitopathy.
The role of TyG index in predicting the incidence of diabetes in Chinese elderly men: a 20-year retrospective study
Authors: Rong Lingjun , Hou Naijing , Hu Jingsheng , Gong Yanping , Yan Shuangtong , Li Chunlin , Yang Zaigang , Sun Banruo
Published: June 2023

Background

The triglyceride glucose index (TyG index) has been regarded as a reliable surrogate marker of insulin resistance and an independent predictor of diabetes. However, few studies have reported the association between the TyG index and diabetes in the elderly population. Accordingly, this study aimed to investigate the association between the TyG index and diabetes progression in elderly Chinese.

Methods

Baseline medical history, fasting plasma glucose (FPG), glucose levels during the oral glucose tolerance test (OGTT) after 1-hour (1h-PG) and 2-hour (2h-PG), and triglyceride (TG) were obtained from a cohort of 862 elderly (aged ≥ 60 years) Chinese in the Beijing urban area between 1998 and 1999. A follow-up visit was conducted between 1998 and 2019 to assess incident diabetes. TyG index was calculated by the following formula ln[TG (mg/dL) × FPG (mg(dL)/2]. The predictive values of TyG index, lipids, and glucose levels during OGTT were assessed alone and also in a clinical prediction model comprising traditional risk factors using concordance index (C-index). Areas under the receiver operating characteristics curves (AUC) and 95% CIs were calculated.

Results

After 20 years of follow-up, there were 544 cases of incident type 2 diabetes mellitus (63.1% of incidence). The multivariable HRs (95% CI) for TyG index, FPG, 1h-PG and 2h-PG, high-density lipoprotein-cholesterol (HDL-c), and TG were 1.525 (1.290-1.804), 1.350 (1.181-1.544), 1.337 (1.282-1.395), 1.401 (1.327-1.480), 0.505 (0.375-0.681), and 1.120 (1.053-1.192), respectively. The corresponding C-index were 0.623, 0.617, 0.704, 0.694, 0.631, and 0.610, respectively. The AUC (95% CI) for the TyG index, FPG, 1h-PG, 2h-PG, HDL-c, and TG were 0.608 (0.569-0.647), 0.587 (0.548-0.625), 0.766 (0.734-0.797), 0.713 (0.679-0.747), 0.397 (0.358-0.435), and 0.588 (0.549-0.628). The AUC of the TyG index was higher than that of TG but did not differ with FPG and HDL-c. In addition, the AUCs of 1h-PG and 2h-PG were higher than that of the TyG index.

Conclusions

Elevated TyG index is independently correlated with an increased risk of incident diabetes in the elderly male population, but it is not superior to OGTT 1h-PG and 2h-PG in predicting the risk of diabetes.
Association between neutrophil to lymphocyte ratio and erectile dysfunction among US males: a population-based cross-sectional study
Authors: Feng Xingliang , Mei Yangyang , Wang Xiaogang , Cui Li , Xu Renfang
Published: June 2023

Objective

The purpose of the study was to investigate the relationship between neutrophil-to-lymphocyte ratio (NLR) and erectile dysfunction (ED) in adult American males using a large database.

Methods

We adopted a series of statistical analyses of the relationship between NLR indices and ED prevalence among participants in the 2001-2004 National Health and Nutrition Examination Survey (NHANES) database using the R software.

Results

The study included a total of 3012 participants, of whom 570 (18.9%) presented with ED. NLR levels were 2.13 (95% CI: 2.08,2.17) in those without ED and 2.36 (95% CI: 2.27,2.45) in those with ED. After adjusting for confounding variables, NLR levels were higher in patients with ED, (β, 1.21, 95% CI, 1.09-1.34, P < 0.001). In addition, a U-shaped relationship between NLR and ED was observed after controlling for all confounders. A more significant correlation (β, 1.35, 95% CI, 1.19 to 1.53, P < 0.001) existed to the right of the inflection point (1.52).

Conclusion

The results of the large cross-sectional study showed a statistically significant association between the occurrence of ED and NLR, a simple, inexpensive, and readily available parameter of inflammation, in US adults. Further studies are still needed in the future to validate and replicate our findings and to investigate the specific mechanisms involved.
Enhancing and upgrading biogas and biomethane production in anaerobic digestion: a comprehensive review
Authors: Aworanti Oluwafunmilayo Abiola , Ajani Ayobami Olu , Agbede Oluseye Omotoso , Agarry Samuel Enahoro , Ogunkunle Oyetola , Laseinde Opeyeolu Timothy , Kalam Md Abul , Fattah Islam Md Rizwanul
Published: June 2023

Anaerobic digestion (AD) processes can face operational challenges or flaws such as substrate structure and characteristics complexity, process complexity, low productivity, inefficient biodegradability, and poor stability, which suppresses or reduces biogas and biomethane production. As a result of the need to overcome these challenges/shortcomings and improve or enhance biogas and biomethane yield, process intensification methods have gained attention. There is some literature review on pretreatment and co-digestion as a means of improving AD performance; however, there is no systematic information on the various strategies required for improving AD performance and, in turn, increasing biogas/biomethane yield. The AD process produces biogas, a valuable renewable biofuel. Biogas is composed primarily of biomethane and other undesirable components such as carbon dioxide, oxygen, hydrogen sulphide, water vapour, ammonia, siloxanes, nitrogen, hydrocarbons, and carbon monoxide, which act as impurities or contaminants and tend to reduce the biogas specific calorific value while also causing various problems with machine operation. As a result, various technologies are used to improve raw biogas quality by removing contaminants during biogas transformation to biomethane. As a result, this paper provides a comprehensive review of the various systematic process intensification strategies used to overcome AD process challenges/shortfalls, improve or enhance biogas and biomethane production, and conventional and emerging or advanced technologies for biogas purification, cleaning, and upgrading.
Analysis of various degradations of five years aged mono c-Si, poly c-Si, and thin-film photovoltaic modules from rooftop solar installations in Dhaka’s tropical wet and dry climate conditions
Authors: Hossion Abul , Manoj Kumar Nallapaneni , Bajaj Mohit , Alrashed Mohammed M. , Elnaggar Mohamed F. , Kamel Salah
Published: June 2023

This study evaluates the degradation of mono, poly, and thin-film silicon solar photovoltaic (PV) modules through visual and electrical measurements in Dhaka’s tropical wet and dry climate conditions. For this, several sites of rooftop solar PV installations in Dhaka, aged at least 5 years, were selected for field investigation. A visible inspection for visual degradation followed by experimental testing on various electrical characteristics of the PV modules installed on the rooftop using a portable Seaward I–V Tracer for measuring the electrical degradation was done. The electrical data were used to evaluate the degradation of short circuit current, open circuit voltage, fill factor, and maximum power in the field as compared to the nameplate values. Experimental results showed the varying types of visual degradation types such as visible cracks, snail trails, moisture intrusion, front glass, and discoloration for the investigated PV modules. The degradation of PV modules in terms of short circuit current was 0.7%/year for 85Wp mono c-Si, 7.6%/year for 150Wp poly c-Si, and 9.7%/year for 105Wp silicon thin-film, respectively. We believe this study would guide the maintenance of the solar PV systems installed on Dhaka city’s rooftop buildings.
The impact of the collaborative innovation network embeddedness on enterprise green innovation performance
Authors: Li Xinmiao , Liu Xipeng
Published: June 2023

As the market environment becomes increasingly competitive, enterprises that rely solely on internal research and development innovation are no longer sufficient to meet the demands of competition. Consequently, enterprises have broken down organizational boundaries and shifted from closed innovation to open collaborative innovation. The flow of knowledge across organizations facilitates the acquisition of heterogeneous resources from partners, promotes the integration and configuration of internal and external knowledge, thereby enhances the competitiveness of enterprises. However, some scholars argued that collaborative innovation does not always achieve win-win outcomes, and the existence of substitution effects between enterprises and their partners may hinder the innovation level of the focal enterprise. Therefore, based on the resource-based theory and the network embeddedness theory, this study constructs a theoretical model to investigate the effects of network embeddedness, network experience and partner diversity on enterprise green innovation performance in the Chinese green collaborative innovation network. The impact of network embeddedness on innovation performance is examined from two dimensions: structural embeddedness and relational embeddedness. The moderating effects of network experience and partner diversity are analyzed to further explain this phenomenon. Using Chinese green patent data from 2000 to 2015 as the research object, the collaborative innovation network of enterprises is constructed, and the network characteristic variables are calculated using social network analysis methods. Finally, negative binomial regression and robustness tests are conducted using STATA software. The research findings provide managerial implications for Chinese enterprises to achieve competitiveness and sustainable development.
Leaching of chemicals and DOC from tire particles under simulated marine conditions
Authors: Foscari Aurelio , Schmidt Natascha , Seiwert Bettina , Herzke Dorte , Sempéré Richard , Reemtsma Thorsten
Published: June 2023

Tire wear particles (TWPs) represent one of the major anthropogenic pools of particles ending up in the environment. They contain a large variety of chemicals, a part of which may be released into the environment through leaching, although the influence of sunlight and other environmental factors during this process is still unclear. This laboratory study compares the leaching of organic compounds from TWP in seawater in the dark and under artificial sunlight for 1) cryo-milled tire tread (CMTT), 2) ‘virgin’ crumb rubber (VCR) and 3) crumb rubber immersed in the sea for ≥12 months prior to the experiments (WCR). Leachates were analyzed for dissolved organic carbon (DOC) and 19 tire-derived chemicals, benzothiazoles and phenylguanidines as well as phenylendiamines by liquid chromatography-high resolution-mass spectrometry. For DOC and most chemicals, the amounts released decreased in the order CMTT > VCR > WCR and increased when leaching occurred under artificial sunlight. sunlight also led to the formation of 23 transformation processes related to 1,3-diphenylguanidine (DPG). In contrast, 4-hydroxydiphenylamine (4-HDPA) and N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine quinone (6-PPDQ) were found in lower amounts upon sunlight exposure. The 19 quantified chemicals, however, did only account for 6%–55% of the DOC in the leachates; most of the DOC, thus, remained unexplained. This study highlights that the amount of chemicals leached from tire particles depends upon their aging history and may be modulated by environmental conditions.
Disentangling the impacts of macroalgae on corals via effects on their microbiomes
Authors: Clements Cody S. , Hay Mark E.
Published: June 2023

Tropical reefs are commonly transitioning from coral to macroalgal dominance, but the role of macroalgae in coral decline remains inadequately understood. A growing body of research suggests that algae may harm corals via disruptions to the homeostasis of the coral holobiont, including resident microbial communities, but the processes that mediate these potential microbial effects and the spatial scales at which they operate are uncertain. Resolving the relative importance and context dependencies of microbially-mediated algal-coral competition is critical for understanding and predicting coral dynamics as reefs further degrade. In this review, we examine the current state of knowledge surrounding algal impacts on corals via disruption of their microbiomes, with a particular focus on the mechanisms hypothesized to mediate microbial effects, the scales at which they are thought to operate, and the evidence from laboratory- and field-based studies for their existence and ecological relevance in the wild. Lastly, we highlight challenges for further advancing the field.
Exploring ‘endangered living fossils’ (ELFs) among monotypic genera of plants and animals of the world
Authors: Vargas Pablo
Published: June 2023

The recently proposed concept of ´endangered living fossils’ (ELFs) integrates high-endangered status and evolutionary singularity for any species. In this review, I gathered monotypic genera (single-species genera) that satisfy the three ELF criteria: (i) scarcity and narrow distribution of populations, i.e., considering every species categorized ‘critically endangered’ or contemporary ´extinct´ by IUCN criteria; (ii) evolutionary singularity, i.e., both morphological and phylogenetic singularities of a single-species lineage as a result of a null net diversification rate; and (iii) ancient divergence, i.e., split from the closest extant relatives predating a particular geological epoch. A total of 3,706 monotypic genera of vertebrates and angiosperms were analyzed. I found 109 critically endangered and contemporary extinct genera of which 57 were ELFs. The emergent patterns are: (1) taxonomy (generic level) is a reliable first approach to identifying ELFs; (2) ´morphological singularity´ displayed by monotypic genera does not always help identify ELFs on islands; (3) species of monotypic genera tend to be more threatened than average species; (4) extinction appears to be biased against some animal and plant groups; (5) contemporary extinct genera are strongly associated with distribution on islands, particularly for flightless birds vulnerable to human prosecution; and (6) the ELF approach is a relatively quick method to identify the species of floras and faunas most urgently in need of protection in the world. This approach is complementary to any method searching for phylogenetic diversity (e. g. EDGE), which is also discussed. I argue that ELFs should be prioritized in conservation because they are the most threatened lineages representing an exceptional evolutionary heritage in the world.
Cooperative, Connected and Automated Mobility Service Continuity in a Cross-Border Multi-Access Edge Computing Federation Scenario
Authors: Hosseini Seyed M. , Jooriah Mohannad , Rocha David , Almeida João , Bartolomeu Paulo , Ferreira Joaquim , Rosales Carlos , Miranda Marta
Published: June 2023

The paradigm shift in transportation systems towards Cooperative, Connected and Automated Mobility (CCAM) aims to improve vehicle maneuverability, reduce pollution, and increase safety. CCAM is primarily responsible for ensuring the best mobility environment, making it one of the main trends in the automotive industry. However, taking advantage of CCAM in Cross-Border Corridors (CBCs) faces many challenges, which go beyond infrastructure deployment cost, and all of which are related to supporting Service Continuity (SC) for mobile users, especially in light of the diversity of territories, network coverage areas, international roaming agreements, and type of cooperative maneuvers. As a solution to these challenges, the paper proposes and implements a new architecture for CCAM SC in CBCs that combines a federation of Multi-access Edge Computing (MEC) concepts to maintain SC.
Nonclassical monocytes potentiate anti-tumoral CD8+ T cell responses in the lungs
Authors: Padgett Lindsey E. , Marcovecchio Paola M. , Olingy Claire E. , Araujo Daniel J. , Steel Kathleen , Dinh Huy Q. , Alimadadi Ahmad , Zhu Yanfang Peipei , Meyer Melissa A. , Kiosses William B. , Thomas Graham D. , Hedrick Catherine C.
Published: June 2023

CD8+ T cells drive anti-cancer immunity in response to antigen-presenting cells such as dendritic cells and subpopulations of monocytes and macrophages. While CD14+ classical monocytes modulate CD8+ T cell responses, the contributions of CD16+ nonclassical monocytes to this process remain unclear. Herein we explored the role of nonclassical monocytes in CD8+ T cell activation by utilizing E2-deficient (E2-/-) mice that lack nonclassical monocytes. During early metastatic seeding, modeled by B16F10-OVA cancer cells injected into E2-/- mice, we noted lower CD8+ effector memory and effector T cell frequencies within the lungs as well as in lung-draining mediastinal lymph nodes in the E2-/- mice. Analysis of the myeloid compartment revealed that these changes were associated with depletion of MHC-IIloLy6Clo nonclassical monocytes within these tissues, with little change in other monocyte or macrophage populations. Additionally, nonclassical monocytes preferentially trafficked to primary tumor sites in the lungs, rather than to the lung-draining lymph nodes, and did not cross-present antigen to CD8+ T cells. Examination of the lung microenvironment in E2-/- mice revealed reduced CCL21 expression in endothelial cells, which is chemokine involved in T cell trafficking. Our results highlight the previously unappreciated importance of nonclassical monocytes in shaping the tumor microenvironment via CCL21 production and CD8+ T cell recruitment.
Expression of lymphoid structure-associated cytokine/chemokine gene transcripts in tumor and protein in serum are prognostic of melanoma patient outcomes
Authors: Karapetyan Lilit , AbuShukair Hassan M. , Li Aofei , Knight Andrew , Al Bzour Ayah Nedal , MacFawn Ian P. , Thompson Zachary J. , Chen Ann , Yang Xi , Dadey Rebekah , Karunamurthy Arivarasan , De Stefano Danielle Vargas , Sander Cindy , Kunning Sheryl R. , Najjar Yana G. , Davar Diwakar , Luke Jason J. , Gooding William , Bruno Tullia C. , Kirkwood John M. , Storkus Walter J.
Published: June 2023

Background

Proinflammatory chemokines/cytokines support development and maturation of tertiary lymphoid structures (TLS) within the tumor microenvironment (TME). In the current study, we sought to investigate the prognostic value of TLS-associated chemokines/cytokines (TLS-kines) expression levels in melanoma patients by performing serum protein and tissue transcriptomic analyses, and to then correlate these data with patients clinicopathological and TME characteristics.

Methods

Levels of TLS-kines in patients’ sera were quantitated using a custom Luminex Multiplex Assay. The Cancer Genomic Atlas melanoma cohort (TCGA-SKCM) and a Moffitt Melanoma cohort were used for tissue transcriptomic analyses. Associations between target analytes and survival outcomes, clinicopathological variables, and correlations between TLS-kines were statistically analyzed.

Results

Serum of 95 patients with melanoma were evaluated; 48 (50%) female, median age of 63, IQR 51-70 years. Serum levels of APRIL/TNFSF13 were positively correlated with levels of both CXCL10 and CXCL13. In multivariate analyses, high levels of serum APRIL/TNFSF13 were associated with improved event-free survival after adjusting for age and stage (HR = 0.64, 95% CI 0.43-0.95; p = 0.03). High expression of APRIL/TNFSF13 tumor transcripts was significantly associated with improved OS in TCGA-SKCM (HR = 0.69, 95% CI 0.52-0.93; p = 0.01) and in Moffitt Melanoma patients (HR = 0.51, 95% CI: 0.32-0.82; p = 0.006). Further incorporation of CXCL13 and CXCL10 tumor transcript levels in a 3-gene index revealed that high APRIL/CXCL10/CXCL13 expression was associated with improved OS in the TCGA SKCM cohort (HR = 0.42, 95% CI 0.19-0.94; p = 0.035). Melanoma differentially expressed genes positively associated with high APRIL/CXCL10/CXCL13 tumor expression were linked to tumor infiltration by a diverse array of proinflammatory immune cell types.

Conclusion

Serum protein and tumor transcript levels of APRIL/TNFSF13 are associated with improved survival outcomes. Patients exhibiting high coordinate expression of APRIL/CXCL10/CXCL13 transcripts in their tumors displayed superior OS. Further investigation of TLS-kine expression profiles related to clinical outcomes in larger cohort studies is warranted.
Exploring the potential common denominator pathogenesis of system lupus erythematosus with COVID-19 based on comprehensive bioinformatics analysis
Authors: Zeng Huiqiong , Zhuang Yu , Li Xiaojuan , Yin Zhihua , Huang Xia , Peng Haiyan
Published: June 2023

Objective

Evidences show that there may be a link between SLE and COVID-19. The purpose of this study is to screen out the diagnostic biomarkers of systemic lupus erythematosus (SLE) with COVID-19 and explore the possible related mechanisms by the bioinformatics approach.

Methods

SLE and COVID-19 datasets were extracted separately from the NCBI Gene Expression Omnibus (GEO) database. The limma package in R was used to obtain the differential genes (DEGs). The protein interaction network information (PPI) and core functional modules were constructed in the STRING database using Cytoscape software. The hub genes were identified by the Cytohubba plugin, and TF-gene together with TF-miRNA regulatory networks were constructed via utilizing the Networkanalyst platform. Subsequently, we generated subject operating characteristic curves (ROC) to verify the diagnostic capabilities of these hub genes to predict the risk of SLE with COVID-19 infection. Finally, a single-sample gene set enrichment (ssGSEA) algorithm was used to analyze immune cell infiltration.

Results

A total of 6 common hub genes (CDC6, PLCG1, KIF15, LCK, CDC25C, and RASGRP1) were identified with high diagnostic validity. These gene functional enrichments were mainly involved in cell cycle, and inflammation-related pathways. Compared to the healthy controls, abnormal infiltration of immune cells was found in SLE and COVID-19, and the proportion of immune cells linked to the 6 hub genes.

Conclusion

Our research logically identified 6 candidate hub genes that could predict SLE complicated with COVID-19. This work provides a foothold for further study of potential pathogenesis in SLE and COVID-19.