bluuebunny/crossref_metadata_2025 huggingface dataset:
Ultrafast energy quenching mechanism of LHCSR3-dependent photoprotection in Chlamydomonas
Authors: Zheng Mengyuan , Pang Xiaojie , Chen Ming , Tian Lijin
Published: May 2024

Photosynthetic organisms have evolved an essential energy-dependent quenching (qE) mechanism to avoid any lethal damages caused by high light. While the triggering mechanism of qE has been well addressed, candidates for quenchers are often debated. This lack of understanding is because of the tremendous difficulty in measuring intact cells using transient absorption techniques. Here, we have conducted femtosecond pump-probe measurements to characterize this photophysical reaction using micro-sized cell fractions of the green alga Chlamydomonas reinhardtii that retain physiological qE function. Combined with kinetic modeling, we have demonstrated the presence of an ultrafast excitation energy transfer (EET) pathway from Chlorophyll a (Chl a) Qy to a carotenoid (car) S1 state, therefore proposing that this carotenoid, likely lutein1, is the quencher. This work has provided an easy-to-prepare qE active thylakoid membrane system for advanced spectroscopic studies and demonstrated that the energy dissipation pathway of qE is evolutionarily conserved from green algae to land plants.
Transparent radiative cooling cover window for flexible and foldable electronic displays
Authors: Lee Kang Won , Yi Jonghun , Kim Min Ku , Kim Dong Rip
Published: May 2024

Transparent radiative cooling holds the promise to efficiently manage thermal conditions in various electronic devices without additional energy consumption. Radiative cooling cover windows designed for foldable and flexible displays could enhance cooling capacities in the ubiquitous deployment of flexible electronics in outdoor environments. However, previous demonstrations have not met the optical, mechanical, and moisture-impermeable criteria for such cover windows. Herein, we report transparent radiative cooling metamaterials with a thickness of 50 microns as a cover window of foldable and flexible displays by rational design and synthesis of embedding optically-modulating microstructures in clear polyimide. The resulting outcome not only includes excellent light emission in the atmospheric window under the secured optical transparency but also provides enhanced mechanical and moisture-impermeable properties to surpass the demands of target applications. Our metamaterials not only substantially mitigate the temperature rise in heat-generating devices exposed to solar irradiance but also enhance the thermal management of devices in dark conditions. The light output performance of light-emitting diodes in displays on which the metamaterials are deployed is greatly enhanced by suppressing the performance deterioration associated with thermalization.
Unravelling the spatial directionality of urban mobility
Authors: Zhao Pengjun , Wang Hao , Liu Qiyang , Yan Xiao-Yong , Li Jingzhong
Published: May 2024

As it is central to sustainable urban development, urban mobility has primarily been scrutinised for its scaling and hierarchical properties. However, traditional analyses frequently overlook spatial directionality, a critical factor in city centre congestion and suburban development. Here, we apply vector computation to unravel the spatial directionality of urban mobility, introducing a two-dimensional anisotropy-centripetality metric. Utilising travel data from 90 million mobile users across 60 Chinese cities, we effectively quantify mobility patterns through this metric, distinguishing between strong monocentric, weak monocentric, and polycentric patterns. Our findings highlight a notable difference: residents in monocentric cities face increasing commuting distances as cities expand, in contrast to the consistent commuting patterns observed in polycentric cities. Notably, mobility anisotropy intensifies in the outskirts of monocentric cities, whereas it remains uniform in polycentric settings. Additionally, centripetality wanes as one moves from the urban core, with a steeper decline observed in polycentric cities. Finally, we reveal that employment attraction strength and commuting distance scaling are key to explaining these divergent urban mobility patterns. These insights are important for shaping effective policies aimed at alleviating congestion and guiding suburban housing development.
Deciphering bat influenza H18N11 infection dynamics in male Jamaican fruit bats on a single-cell level
Authors: Kessler Susanne , Burke Bradly , Andrieux Geoffroy , Schinköthe Jan , Hamberger Lea , Kacza Johannes , Zhan Shijun , Reasoner Clara , Dutt Taru S. , Kaukab Osman Maria , Henao-Tamayo Marcela , Staniek Julian , Villena Ossa Jose Francisco , Frank Dalit T. , Ma Wenjun , Ulrich Reiner , Cathomen Toni , Boerries Melanie , Rizzi Marta , Beer Martin , Schwemmle Martin , Reuther Peter , Schountz Tony , Ciminski Kevin
Published: May 2024

Jamaican fruit bats (Artibeus jamaicensis) naturally harbor a wide range of viruses of human relevance. These infections are typically mild in bats, suggesting unique features of their immune system. To better understand the immune response to viral infections in bats, we infected male Jamaican fruit bats with the bat-derived influenza A virus (IAV) H18N11. Using comparative single-cell RNA sequencing, we generated single-cell atlases of the Jamaican fruit bat intestine and mesentery. Gene expression profiling showed that H18N11 infection resulted in a moderate induction of interferon-stimulated genes and transcriptional activation of immune cells. H18N11 infection was predominant in various leukocytes, including macrophages, B cells, and NK/T cells. Confirming these findings, human leukocytes, particularly macrophages, were also susceptible to H18N11, highlighting the zoonotic potential of this bat-derived IAV. Our study provides insight into a natural virus-host relationship and thus serves as a fundamental resource for future in-depth characterization of bat immunology.
Internal water circulation mediated synergistic co-hydrolysis of PET/cotton textile blends in gamma-valerolactone
Authors: Zhang Shun , Xu Wenhao , Du Rongcheng , Yan Lei , Liu Xuehui , Xu Shimei , Wang Yu-Zhong
Published: May 2024

Recycling strategies for mixed plastics and textile blends currently aim for recycling only one of the components. Here, we demonstrate a water coupling strategy to co-hydrolyze polyester/cotton textile blends into polymer monomers and platform chemicals in gamma-valerolactone. The blends display a proclivity for achieving an augmented 5-hydroxymethylfurfural yield relative to the degradation of cotton alone. Controlled experiments and preliminary mechanistic studies underscore that the primary driver behind this heightened conversion rate lies in the internal water circulation. The swelling and dissolving effect of gamma-valerolactone on polyester enables a fast hydrolysis of polyester at much lower concentration of acid than the one in the traditional hydrolysis methods, effectively mitigating the excessive degradation of cotton-derived product and undesirable product formation. In addition, the system is also applicable to different kinds of blends and PET mixed plastics. This strategy develops an attractive path for managing end-of-life textiles in a sustainable and efficient way.
Exploring brain plasticity in developmental dyslexia through implicit sequence learning
Authors: Olivo Gaia , Persson Jonas , Hedenius Martina
Published: May 2024

Developmental dyslexia (DD) is defined as difficulties in learning to read even with normal intelligence and adequate educational guidance. Deficits in implicit sequence learning (ISL) abilities have been reported in children with DD. We investigated brain plasticity in a group of 17 children with DD, compared with 18 typically developing (TD) children, after two sessions of training on a serial reaction time (SRT) task with a 24-h interval. Our outcome measures for the task were: a sequence-specific implicit learning measure (ISL), entailing implicit recognition and learning of sequential associations; and a general visuomotor skill learning measure (GSL). Gray matter volume (GMV) increased, and white matter volume (WMV) decreased from day 1 to day 2 in cerebellar areas regardless of group. A moderating effect of group was found on the correlation between WMV underlying the left precentral gyrus at day 2 and the change in ISL performance, suggesting the use of different underlying learning mechanisms in DD and TD children during the ISL task. Moreover, DD had larger WMV in the posterior thalamic radiation compared with TD, supporting previous reports of atypical development of this structure in DD. Further studies with larger sample sizes are warranted to validate these results.
Enhancing mass spectrometry imaging accessibility using convolutional autoencoders for deriving hypoxia-associated peptides from tumors
Authors: Bitto Verena , Hönscheid Pia , Besso María José , Sperling Christian , Kurth Ina , Baumann Michael , Brors Benedikt
Published: May 2024

Mass spectrometry imaging (MSI) allows to study cancer’s intratumoral heterogeneity through spatially-resolved peptides, metabolites and lipids. Yet, in biomedical research MSI is rarely used for biomarker discovery. Besides its high dimensionality and multicollinearity, mass spectrometry (MS) technologies typically output mass-to-charge ratio values but not the biochemical compounds of interest. Our framework makes particularly low-abundant signals in MSI more accessible. We utilized convolutional autoencoders to aggregate features associated with tumor hypoxia, a parameter with significant spatial heterogeneity, in cancer xenograft models. We highlight that MSI captures these low-abundant signals and that autoencoders can preserve them in their latent space. The relevance of individual hyperparameters is demonstrated through ablation experiments, and the contribution from original features to latent features is unraveled. Complementing MSI with tandem MS from the same tumor model, multiple hypoxia-associated peptide candidates were derived. Compared to random forests alone, our autoencoder approach yielded more biologically relevant insights for biomarker discovery.
Virus-like structures for combination antigen protein mRNA vaccination
Authors: Zhang Jingjing , Li Yanmei , Zeng Fengyuan , Mu Changyong , Liu Change , Wang Lichun , Peng Xiaowu , He Liping , Su Yanrui , Li Hongbing , Wang An , Feng Lin , Gao Dongxiu , Zhang Zhixiao , Xu Gang , Wang Yixuan , Yue Rong , Si Junbo , Zheng Lichun , Zhang Xiong , He Fuyun , Yi Hongkun , Tang Zhongshu , Li Gaocan , Ma Kaili , Li Qihan
Published: May 2024

Improved vaccination requires better delivery of antigens and activation of the natural immune response. Here we report a lipid nanoparticle system with the capacity to carry antigens, including mRNA and proteins, which is formed into a virus-like structure by surface decoration with spike proteins, demonstrating application against SARS-CoV-2 variants. The strategy uses S1 protein from Omicron BA.1 on the surface to deliver mRNA of S1 protein from XBB.1. The virus-like particle enables specific augmentation of mRNAs expressed in human respiratory epithelial cells and macrophages via the interaction the surface S1 protein with ACE2 or DC-SIGN receptors. Activation of macrophages and dendritic cells is demonstrated by the same receptor binding. The combination of protein and mRNA increases the antibody response in BALB/c mice compared with mRNA and protein vaccines alone. Our exploration of the mechanism of this robust immunity suggests it might involve cross-presentation to diverse subsets of dendritic cells ranging from activated innate immune signals to adaptive immune signals.
A fundus image dataset for intelligent retinopathy of prematurity system
Authors: Zhao Xinyu , Chen Shaobin , Zhang Sifan , Liu Yaling , Hu Yarou , Yuan Duo , Xie Liqiong , Luo Xiayuan , Zheng Mianying , Tian Ruyin , Chen Yi , Tan Tao , Yu Zhen , Sun Yue , Wu Zhenquan , Zhang Guoming
Published: May 2024

Image-based artificial intelligence (AI) systems stand as the major modality for evaluating ophthalmic conditions. However, most of the currently available AI systems are designed for experimental research using single-central datasets. Most of them fell short of application in real-world clinical settings. In this study, we collected a dataset of 1,099 fundus images in both normal and pathologic eyes from 483 premature infants for intelligent retinopathy of prematurity (ROP) system development and validation. Dataset diversity was visualized with a spatial scatter plot. Image classification was conducted by three annotators. To the best of our knowledge, this is one of the largest fundus datasets on ROP, and we believe it is conducive to the real-world application of AI systems.
Development of a novel Colorimetric Assay for the rapid diagnosis of Coronavirus disease 2019 from nasopharyngeal samples
Authors: Sepahi Neda , Samsami Sahar , Mansoori Yaser , Chenari Maryam , Namavari Negin , Yazdanpanah Ava , Ghasemian Abdolmajid , Montaseri Zahra , Sharifzadeh Mahboobeh , Ranjbar Razie , Namavari Sahar , Ghanbariasad Ali
Published: May 2024

Emergence of Coronavirus disease 2019 (COVID-19) pandemic has posed a huge threat to public health. Rapid and reliable test to diagnose infected subjects is crucial for disease spread control. We developed a colorimetric test for COVID-19 detection using a Colorimetric Assay based on thiol-linked RNA modified gold nanoparticles (AuNPs) and oligonucleotide probes. This method was conducted on RNA from 200 pharyngeal swab samples initially tested by Real-Time polymerase chain reaction (RT-PCR) as gold standard. A specific oligonucleotide probe designed based on ORF1ab of COVID-19 was functionalized with AuNPs-probe conjugate. The exposure of AuNP-probe to isolated RNA samples was tested using hybridization. In this comparative study, the colorimetric functionalized AuNPs assay exhibited a detection limit of 25 copies/µL. It was higher in comparison to the RT-PCR method, which could only detect 15 copies/µL. The results demonstrated 100% specificity and 96% sensitivity for the developed method. Herein, we developed an incredibly rapid, simple and cost-effective Colorimetric Assay lasting approximately 30 min which could process considerably higher number of COVID-19 samples compared to the RT-PCR. This AuNP-probe conjugate colorimetric method could be considered the optimum alternatives for conventional diagnostic tools especially in over-populated and/or low-income countries.
Network pharmacology, molecular docking, and dynamics analyses to predict the antiviral activity of ginger constituents against coronavirus infection
Authors: Samy Asmaa , Hassan Afnan , Hegazi Nesrine M. , Farid Mai , Elshafei Moustafa
Published: May 2024

COVID-19 is a global pandemic that caused a dramatic loss of human life worldwide, leading to accelerated research for antiviral drug discovery. Herbal medicine is one of the most commonly used alternative medicine for the prevention and treatment of many conditions including respiratory system diseases. In this study, a computational pipeline was employed, including network pharmacology, molecular docking simulations, and molecular dynamics simulations, to analyze the common phytochemicals of ginger rhizomes and identify candidate constituents as viral inhibitors. Furthermore, experimental assays were performed to analyze the volatile and non-volatile compounds of ginger and to assess the antiviral activity of ginger oil and hydroalcoholic extract. Network pharmacology analysis showed that ginger compounds target human genes that are involved in related cellular processes to the viral infection. Docking analysis highlighted five pungent compounds and zingiberenol as potential inhibitors for the main protease (Mpro), spike receptor-binding domain (RBD), and human angiotensin-converting enzyme 2 (ACE2). Then, (6)-gingerdiacetate was selected for molecular dynamics (MD) simulations as it exhibited the best binding interactions and free energies over the three target proteins. Trajectories analysis of the three complexes showed that RBD and ACE2 complexes with the ligand preserved similar patterns of root mean square deviation (RMSD) and radius of gyration (Rg) values to their respective native structures. Finally, experimental validation of the ginger hydroalcoholic extract confirmed the existence of (6)-gingerdiacetate and revealed the strong antiviral activity of the hydroalcoholic extract with IC$$_{50}$$

50

of 2.727 $$\upmu \hbox {g}/\hbox {ml}$$

μ
g
/
ml

. Our study provides insights into the potential antiviral activity of (6)-gingerdiacetate that may enhance the host immune response and block RBD binding to ACE2, thereby, inhibiting SARS-CoV-2 infection.
Voronoi cell finite element method for heat conduction analysis of composite materials
Authors: Chen Siqi , Hu Changhao , Tian Jingjie , Tan Dawen , Gong Yuqiang , Xia Fan , Ning Shaoqing , Zhang Rui
Published: May 2024

In this paper, Voronoi cell finite element method (VCFEM) based on assumed flux hybrid formulation has been presented for heat conduction problem of particle reinforced composites material. The heat fluxes satisfying a priori internal thermal balance are directly approximated independently in the matrix and the inclusion respectively. The temperatures on element boundary and matrix-inclusion interface are interpolated by nodal temperature. The thermal balance on the interelement boundary and matrix-inclusion interface is relaxed and introduced into the functional by taking the temperature as Lagrange multiplier. In this way, a functional containing two variables of heat flux and temperature is proposed. Full field heat flux and effective thermal conductivity are obtained. Feasibility and effectiveness of the proposed approach are verified through several numerical examples.
Glucose uptake in trophoblasts of GDM mice is regulated by the AMPK-CLUT3 signaling pathway
Authors: Xiao Zhenghua , Liu Xue , Luan Xiaojin , Duan Ran , Peng Wei , Tong Chao , Qiao Juan , Qi Hongbo
Published: May 2024

GDM, as a metabolic disease during pregnancy, regulates GLUT3 translocation by AMPK, thereby affecting glucose uptake in trophoblasts. It provides a new research idea and therapeutic target for alleviating intrauterine hyperglycemia in GDM. STZ was used to construct GDM mice, inject AICAR into pregnant mice, and observe fetal and placental weight; flow cytometry was employed for the detection of glucose uptake by primary trophoblast cells; immunofluorescence was applied to detect the localization of GLUT3 and AMPK in placental tissue; Cocofal microscope was used to detect the localization of GLUT3 in trophoblast cells;qRT-PCR and Western blot experiments were carried out to detect the expression levels of GLUT3 and AMPK in placental tissue; CO-IP was utilized to detect the interaction of GLUT3 and AMPK. Compared with the normal pregnancy group, the weight of the fetus and placenta of GDM mice increased (P < 0.001), and the ability of trophoblasts to take up glucose decreased (P < 0.001). In addition, AMPK activity in trophoblasts and membrane localization of GLUT3 in GDM mice were down-regulated compared with normal pregnant mice (P < 0.05). There is an interaction between GLUT3 and AMPK. Activating AMPK in trophoblasts can up-regulate the expression of GLUT3 membrane protein in trophoblasts of mice (P < 0.05) and increase the glucose uptake of trophoblasts (P < 0.05). We speculate that inhibition of AMPK activity in GDM mice results in aberrant localization of GLUT3, which in turn attenuates glucose uptake by placental trophoblast cells. AICAR activates AMPK to increase the membrane localization of GLUT3 and improve the glucose uptake capacity of trophoblasts.
AI-based model for automatic identification of multiple sclerosis based on enhanced sea-horse optimizer and MRI scans
Authors: Khattap Mohamed G. , Abd Elaziz Mohamed , Hassan Hend Galal Eldeen Mohamed Ali , Elgarayhi Ahmed , Sallah Mohammed
Published: May 2024

This study aims to develop an AI-enhanced methodology for the expedited and accurate diagnosis of Multiple Sclerosis (MS), a chronic disease affecting the central nervous system leading to progressive impairment. Traditional diagnostic methods are slow and require substantial expertise, underscoring the need for innovative solutions. Our approach involves two phases: initially, extracting features from brain MRI images using first-order histograms, the gray level co-occurrence matrix, and local binary patterns. A unique feature selection technique combining the Sine Cosine Algorithm with the Sea-horse Optimizer is then employed to identify the most significant features. Utilizing the eHealth lab dataset, which includes images from 38 MS patients (mean age 34.1 ± 10.5 years; 17 males, 21 females) and matched healthy controls, our model achieved a remarkable 97.97% detection accuracy using the k-nearest neighbors classifier. Further validation on a larger dataset containing 262 MS cases (199 females, 63 males; mean age 31.26 ± 10.34 years) and 163 healthy individuals (109 females, 54 males; mean age 32.35 ± 10.30 years) demonstrated a 92.94% accuracy for FLAIR images and 91.25% for T2-weighted images with the Random Forest classifier, outperforming existing MS detection methods. These results highlight the potential of the proposed technique as a clinical decision-making tool for the early identification and management of MS.
Age-related changes of intraocular pressure in Dutch belted rabbits
Authors: Shin Young In , Kim Young Kook , Jeoung Jin Wook , Park Ki Ho
Published: May 2024

This study investigated intraocular pressure (IOP) in Dutch belted rabbits using two different tonometers, rebound tonometry (TonoVet Plus; TVP) and a Tonopen (Tono-Pen AVIA Vet; TPA). Post-pubescent male Dutch belted rabbits aged 36 weeks (n = 10 animals) were used in the study. IOP measurements were conducted every 2 weeks for 22 weeks using TVP and TPA on both eyes of each rabbit. The average IOP measurements were compared by the paired Student’s t-test. Pairwise Pearson’s correlation coefficients and Bland–Altman statistics were used. The overall mean IOP measured with TPA was significantly higher than that with TVP (23.5 ± 4.9 vs. 21.8 ± 2.4 mmHg for the right eyes; P = 0.045, and 23.0 ± 4.7 vs. 21.5 ± 2.4 mmHg for the left eyes; P = 0.047). Both tonometers tended to show increased IOP readings with age, and positive correlations between IOP and age were observed with both TPA (r = 0.95, P < 0.001 for right eyes; r = 0.95, P < 0.001 for left eyes) and TVP (r = 0.91, P < 0.001 for right eyes; r = 0.64, P = 0.024 for left eyes). The average bias calculated by subtracting TPA from TVP was − 1.60 (95% confidence intervals − 1.927, − 1.281) mmHg. IOP in post-pubescent Dutch belted rabbits tended to increase with age throughout the 22 week study.
Contrasting mineralized and barren porphyries in the Zhongdian Arc, insights from biotite and apatite compositions and halogen fugacity
Authors: Pan Yanning , Dong Guochen , Tsunogae Toshiaki , Wang Peng , Li Xuefeng , Dong Pengsheng
Published: May 2024

Copper mineralization in the Pulang (PL) porphyry deposit, Langdu (LD) porphyry-skarn deposit and Songnuo (SN) porphyry prospect in northwestern Yunnan, China, is closely related to the emplacement of quartz monzonite porphyries. The chemical compositions of biotite and apatite from those porphyries were analyzed to calculate the halogen fugacity and to constrain mineralized and barren porphyries. Our data show that biotites from the PL deposit have higher MgO, SiO2, TiO2 and F contents than those from the LD deposit or SN prospect. Compared to those in the LD deposit and SN prospect, the Mg (atoms per formula unit (apfu)) and AlVI (apfu) value in biotite is greater, and the F content is greater and the SO3 and Ce2O3/Y2O3 ratio in apatite are lower in the PL deposit. Ti-biotite thermometry and apatite-biotite geothermometry show that the crystallization temperature of biotite from the PL deposit is higher than that from the SN prospect or LD deposit. The results suggest that oxygen fugacity, magmatic sulfur, and H2O contents cannot be used to efficiently distinguish the PL deposit from the LD deposit and SN prospect. However, the halogen chemistry of biotite from the PL deposit is distinctly different from that of the LD deposit or SN prospect according to the lower IV (F), indicating that mineralized quartz monzonite porphyries in the PL deposit formed during the late magmatic stage, which is in contrast to those in the LD deposit and SN prospect. The mineralized porphyries display a remarkable negative linear relationship (r = − 0.96) with the log (f HF/f HCl) and log (f H2O/f HF) ratio, which can be used to distinguish the mineralized and barren porphyries. Compared with other typical porphyry Cu systems, there is a remarkable positive linear relationship between IV (Cl) and log (f H2O/f HCl). In addition, the linear slope and intercept for log (f H2O/f HCl) ratios and the IV (Cl) of biotite from the potassic and phyllic alteration zones are significantly greater than those from other porphyries.
No immediate attentional bias towards or choice bias for male secondary sexual characteristics in Bornean orang-utans (Pongo pygmaeus)
Authors: Roth Tom S. , Samara Iliana , Perea-Garcia Juan Olvido , Kret Mariska E.
Published: May 2024

Primate faces provide information about a range of variant and invariant traits, including some that are relevant for mate choice. For example, faces of males may convey information about their health or genetic quality through symmetry or facial masculinity. Because perceiving and processing such information may have bearing on the reproductive success of an individual, cognitive systems are expected to be sensitive to facial cues of mate quality. However, few studies have investigated this topic in non-human primate species. Orang-utans are an interesting species to test mate-relevant cognitive biases, because they are characterised by male bimaturism: some adult males are fully developed and bear conspicuous flanges on the side of their face, while other males look relatively similar to females. Here, we describe two non-invasive computerised experiments with Bornean orang-utans (Pongo pygmaeus), testing (i) immediate attention towards large flanges and symmetrical faces using a dot-probe task (N = 3 individuals; 2F) and (ii) choice bias for pictures of flanged males over unflanged males using a preference test (N = 6 individuals; 4F). In contrast with our expectations, we found no immediate attentional bias towards either large flanges or symmetrical faces. In addition, individuals did not show a choice bias for stimuli of flanged males. We did find exploratory evidence for a colour bias and energy efficiency trade-offs in the preference task. We discuss our null results and exploratory results in the context of the evolutionary history of Bornean orang-utans, and provide suggestions for a more biocentric approach to the study of orang-utan cognition.
A broadband second-order bandpass frequency selective surface for microwave and millimeter wave application
Authors: Li Zhiming , Weng Xiaolong , Yi Xu , Li Kai , Duan Wei , Bi Mei
Published: May 2024

This paper presents a frequency selective surface (FSS) with a wideband second-order bandpass response in the dual-band of microwave and millimeter wave. The overall structure consists of three layers of metal pattern and two layers of thin dielectric substrate. The top and bottom metal layers have capacitive patches with integrated curled Jerusalem cross slot resonators, while the intermediate metal layer has an inductive grid structure with cross-shaped slot resonators. The incorporated slot resonators play a pivotal role in achieving the desired transmission poles or zeros, which enable a wideband second-order filtering response in the dual-band and a quick roll-off at the passband edges, increasing the efficacy of electromagnetic shielding. To fully investigate the structure's frequency response, an equivalent circuit model of the structure is created, spanning the complete frequency range of 5–50 GHz. Physical samples are created and measured to confirm the suggested approach’s efficacy. The passband center frequencies of the FSS are found at f1 = 19.42 GHz and f2 = 42.78 GHz, and the − 3 dB bandwidth is 4.34 GHz (17.25–21.59 GHz) and 8.54 GHz (38.51–47.05 GHz), respectively. The simulation results align well with the experimental data. The transmission response rapidly transitions from the passband to the stopband at the passband boundaries.
Fano resonance in one-dimensional quasiperiodic topological phononic crystals towards a stable and high-performance sensing tool
Authors: Almawgani Abdulkarem H. M. , Makhlouf Fathy Hamza , E. Alfassam Haifa , M. El-Sherbeeny Ahmed , Hajjiah Ali , A. Elsayed Hussein , R. Abukhadra Mostafa , Al Zoubi Wail , Semeda Ramadan , Ismail Fathy Moataz , A. H. Al-Athwary Anwar , Mehaney Ahmed
Published: May 2024

Phononic crystals (PnCs) emerge as an innovative sensor technology, especially for high-performance sensing applications. This study strives to advance this field by developing new designs of PnC structures that exhibit stability in the face of construction imperfections and deformations, focusing on the evolution of topological PnCs (TPnCs). These designs could be promising to overcome the problem of instability involved in most of the theoretical PnC sensors when they emerge in experimental verification. In particular, the fabrication process of any design could collide with some fluctuations in controlling the size of each component. Thus, Fano resonance is introduced through a one-dimensional (1D) quasiperiodic TPnC. To the best of the author’s knowledge, this study is the first to observe Fano modes in liquid cavities through 1D PnCs. Various quasiperiodic PnC designs are employed to detect the temperature of alcohols (specifically propanol) across an extensive temperature range (160–240 °C). The effects of many geometrical parameters on the sensor stability, such as material thicknesses, are studied. Numerical findings demonstrated that the designed quasiperiodic topological PnCs based on Fibonacci sequence of the second order proved superior performance. This sensing tool provides sensitivity, quality factor and figure-of-merit values of 104,533.33 Hz/°C, 223.69 and 0.5221 (/°C), respectively, through temperature detection of propanol in the range of 160–240 °C.
Research on domain ontology construction based on the content features of online rumors
Authors: Zhao Jianbo , Liu Huailiang , Zhang Weili , Sun Tong , Chen Qiuyi , Wang Yuehai , Cheng Jiale , Zhuang Yan , Zhang Xiaojin , Zhang Shanzhuang , Li Bowei , Ding Ruiyu
Published: May 2024

Online rumors are widespread and difficult to identify, which bring serious harm to society and individuals. To effectively detect and govern online rumors, it is necessary to conduct in-depth semantic analysis and understand the content features of rumors. This paper proposes a TFI domain ontology construction method, which aims to achieve semantic parsing and reasoning of the rumor text content. This paper starts from the term layer, the frame layer, and the instance layer, and based on the reuse of the top-level ontology, the extraction of core literature content features, and the discovery of new concepts in the real corpus, obtains the core classes (five parent classes and 88 subclasses) of the rumor domain ontology and defines their concept hierarchy. Object properties and data properties are designed to describe relationships between entities or their features, and the instance layer is created according to the real rumor datasets. OWL language is used to encode the ontology, Protégé is used to visualize it, and SWRL rules and pellet reasoner are used to mine and verify implicit knowledge of the ontology, and judge the category of rumor text. This paper constructs a rumor domain ontology with high consistency and reliability.
Upscaling irradiation protocols of Aedes albopictus pupae within an SIT program in Reunion Island
Authors: Marquereau Lucie , Yamada Hanano , Damiens David , Leclercq Antonin , Derepas Brice , Brengues Cécile , Dain Brice William , Lejarre Quentin , Proudhon Mickael , Bouyer Jeremy , Gouagna Louis Clément
Published: May 2024

The implementation of the sterile insect technique against Aedes albopictus relies on many parameters, in particular on the success of the sterilization of males to be released into the target area in overflooding numbers to mate with wild females. Achieving consistent sterility levels requires efficient and standardized irradiation protocols. Here, we assessed the effects of exposure environment, density of pupae, irradiation dose, quantity of water and location in the canister on the induced sterility of male pupae. We found that the irradiation of 2000 pupae in 130 ml of water and with a dose of 40 Gy was the best combination of factors to reliably sterilize male pupae with the specific irradiator used in our control program, allowing the sterilization of 14000 pupae per exposure cycle. The location in the canister had no effect on induced sterility. The results reported here allowed the standardization and optimization of irradiation protocols for a Sterile Insect Technique program to control Ae. albopictus on Reunion Island, which required the production of more than 300,000 sterile males per week.
Setting a shared development agenda: prioritizing the sustainable development goals in the Dominican Republic with fuzzy-LMAW
Authors: Fernández-Portillo Luis A. , Demir Gülay , Sianes Antonio , Santos-Carrillo Francisco
Published: May 2024

The sustainable development goals (SDGs) were established by the United Nations as an international call to eradicate poverty, safeguard the environment, and guarantee that everyone lives in peace and prosperity by 2030. The SDGs aim to balance growth and sustainability in three dimensions: social, economic and environmental. However, in the post-pandemic era, when resources for public development policies are scarce, nations face the problem of prioritizing which SDGs to pursue. A lack of agreement is one of the determinants of low performance levels of the SDGs, and multicriteria decision analysis tools can help in this task, which is especially relevant in developing countries that are falling behind in achieving the SDGs. To test the feasibility and appropriateness of one of these tools, the Fuzzy Logarithm Methodology of Additive Weights, we apply it to prioritize the SDGs in the Dominican Republic, to see if the priorities established are consistent. Seventeen experts were surveyed, and the main result was that Decent work and economic growth was the most important goal for the country. Our findings, consistent with the literature, show the path to similar applications in other developing countries to enhance performance levels in the achievement of the SDGs.
Treatment of patients with carcinomas in advanced stages with 5-fluorouracil, folinic acid and pyridoxine in tandem
Authors: Machover David , Almohamad Wathek , Castagné Vincent , Desterke Christophe , Gomez Léa , Goldschmidt Emma
Published: May 2024

The effect of high-dose pyridoxine (PN) on activity of 5-fluorouracil (FUra) and folinic acid (FA)-containing regimens was studied in 50 patients including 14 with digestive tract, and 36 with breast carcinomas (BC) in advanced stages with poor prognostic characteristics. Patients with colorectal, and pancreas adenocarcinoma received oxaliplatin, irinotecan, FUra, FA (Folfirinox), and patients with squamous cell carcinoma of the esophagus had paclitaxel, carboplatin, FUra, FA (TCbF). Patients with BC received AVCF (doxorubicin, vinorelbine, cyclophosphamide, FUra, FA) followed by TCbF or TCbF only, and patients who overexpressed HER2 received TCbF plus trastuzumab and pertuzumab. PN (1000–3000 mg/day iv) preceded each administration of FUra and FA. 47 patients (94%) responded, including 16 (32%) with CR. Median tumor reduction was 93%. Median event-free survival (EFS) was 37.7 months. The 25 patients with tumor shrinkage ≥ 91% had EFS of 52% from 42 months onwards. Unexpected toxicity did not occur. PN enhances potency of chemotherapy regimens comprising FUra and FA.
Association between lipid profile and clinical outcomes in COVID-19 patients
Authors: Ochoa-Ramírez Luis Antonio , De la Herrán Arita Alberto Kousuke , Sanchez-Zazueta Jorge Guillermo , Ríos-Burgueño Efrén , Murillo-Llanes Joel , De Jesús-González Luis Adrián , Farfan-Morales Carlos Noe , Cordero-Rivera Carlos Daniel , del Ángel Rosa María , Romero-Utrilla Alejandra , Camberos-Barraza Josué , Valdez-Flores Marco Antonio , Camacho-Zamora Alejandro , Batiz-Beltrán José Candelario , Angulo-Rojo Carla , Guadrón-Llanos Alma Marlene , Picos-Cárdenas Verónica Judith , Norzagaray-Valenzuela Claudia Desiree , Rábago-Monzón Ángel Radamés , Velarde-Félix Jesús Salvador , Reyes-Ruiz José Manuel , Osuna-Ramos Juan Fidel
Published: May 2024

High-density lipoprotein cholesterol (HDL-c) removes cholesterol, an essential component in lipid rafts, and this cholesterol removal can regulate protein attachment to lipid rafts, modulating their functionality in the immune cell response. Although severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can alter the lipid profile, there is little information on the role of HDL-c and other lipids in prognostic of the coronavirus disease 2019 (COVID-19) in Mexican population. This study aims to evaluate the predictive value of HDL-c and lipid profile on severity and survival of 102 patients infected with SARS-CoV-2 during the COVID-19 first wave. Our findings, derived from univariate and multivariate Cox proportional hazards regression models, highlighted age and hypertension as significant predictors of survival (HR = 1.04, p = 0.012; HR = 2.78, p = 0.027), while gender, diabetes, and obesity showed no significant impact. Triglycerides and HDL-c levels notably influenced mortality, with elevated triglycerides and lower HDL-c associated with higher mortality risk (p = 0.032). This study underscores the importance of lipid profiles alongside traditional risk factors in assessing COVID-19 risk and outcomes. It contributes to the understanding of COVID-19 patient management and emphasizes the need for further investigation into the role of dyslipidemia in influencing COVID-19 prognosis, potentially aiding in refined risk stratification and therapeutic strategies.
Effect of alverine citrate plus simethicone in colonoscopy: a randomized controlled trial
Authors: Wilasrusmee Chumpon , Jirasiritham Jakrapan , Supsamutchai Chairat , Punmeechao Puvee , Poprom Napaphat
Published: May 2024

Colonoscopy is the standard procedure for screening, and surveillance of colorectal cancer, including the treatment for colonic lesions. Colonic spasm is an important problem from colonoscopy that affects both surgeons and patients. The spasm also might be the cause of longer cecal intubation time, difficulty of the procedure, and increased pain. Previous reports indicated that antispasmodic agents can decrease such symptoms. Therefore, we conducted this study to investigate the cecal intubation time of antispasmodic agents. A single blinded randomized controlled trial was conducted from 01/11/2020 to 31/08/2021. One hundred four patients were allocated to antispasmodic agent group and control group, in 1:1 ratio. The efficacy of median (range) cecal intubation time showed similar results of 5 (2, 14) and 5 (2, 15) minutes with no statistically significant difference. The mean scores of all domains i.e., pain, spasm, cleanliness, and difficulty were better in the antispasmodic agent group about 2.6 (1.4), 1.8 (0.8), 2.4 (0.9), and 2.0 (0.9), respectively, than control group but there were spasm and cleanliness showed statistically significant difference. Moreover, the satisfaction scores showed better efficacy in decreased spasm, decreased difficulty, and increased cleanliness than control group. Prescribing of antispasmodic drugs before colonoscopy might be the choice of treatment for the patients. The antispasmodic drugs will be beneficial to both of the patient and the doctor.
YOLOv8-PD: an improved road damage detection algorithm based on YOLOv8n model
Authors: Zeng Jiayi , Zhong Han
Published: May 2024

Road damage detection is an crucial task to ensure road safety. To tackle the issues of poor performance on multi-scale pavement distresses and high costs in detection task, this paper presents an improved lightweight road damage detection algorithm based on YOLOv8n, named YOLOv8-PD (pavement distress). Firstly, a BOT module that can extract global information of road damage images is proposed to adapt to the large-span features of crack objects. Secondly, the introduction of the large separable kernel attention (LKSA) mechanism enhances the detection accuracy of the algorithm. Then, a C2fGhost block is constructed in the neck network to strengthen the feature extraction of complex road damages while reducing the computational load. Furthermore, we introduced lightweight shared convolution detection head (LSCD-Head) to improve feature expressiveness and reduce the number of parameters. Finally, extensive experiments on the RDD2022 dataset yield a model with parametric and computational quantities of 2.3M and 6.1 GFLOPs, which are only 74.1% and 74.3% of the baseline, and the mAP reaches an improvement of 1.4 percentage points from the baseline. In addition, experimental results on the RoadDamage dataset show that the mAP increased by 4.2% and this algorithm has good robustness. This method can provide a reference for the automatic detection method of pavement distress.
Degradation of reactive red (B-3BF) dye wastewater using UV irradiation (254/185 nm) with sodium persulfate in a pilot UV device
Authors: Wang Chao , Li Yongqiang , Wan Junmin , Hu Yi , Huang Yi , Qiu Jiangen
Published: May 2024

Two low-pressure ultraviolet (UV) lamps at 185/254 nm with sodium persulfate in a pilot UV device were utilized for the degradation of reactive red (B-3BF) dye wastewater compared with two UV lamps at 185/185 nm and two UV lamps at 254/254 nm. The degradation performances of UV irradiation (254/185 nm) with sodium persulfate under different degradation times, flow rates, initial pH, initial Na2S2O8 concentrations and initial dye concentrations were investigated. The experimental results illustrated that the degradation percentage of B-3BF dye could reduce to 90.42% with the energy consumption of 85.1 kWh/kg and the residual dye concentration of 1.92 mg/L by UV irradiation (254/185 nm) with initial Na2S2O8 concentration of 1.5 mmol/L and initial dye concentration of 20 mg/L. In addition, degradation performance of B-3BF dye wastewater by UV irradiation (254/185 nm) with sodium persulfate was more effective than those of UV irradiation (254/254 nm) and UV irradiation (185/185 nm). Therefore UV irradiation (254/185 nm) with sodium persulfate was promising for the degradation of B-3BF dye wastewater.
A novel APP splice variant-dependent marker system to precisely demarcate maturity in SH-SY5Y cell-derived neurons
Authors: Kulatunga D. Chanuka M. , Ranaraja Umanthi , Kim Eun Young , Kim Ryoung Eun , Kim Dong Ern , Ji Kuk Bin , Kim Min Kyu
Published: May 2024

SH-SY5Y, a neuroblastoma cell line, can be converted into mature neuronal phenotypes, characterized by the expression of mature neuronal and neurotransmitter markers. However, the mature phenotypes described across multiple studies appear inconsistent. As this cell line expresses common neuronal markers after a simple induction, there is a high chance of misinterpreting its maturity. Therefore, sole reliance on common neuronal markers is presumably inadequate. The Alzheimer's disease (AD) central gene, amyloid precursor protein (APP), has shown contrasting transcript variant dynamics in various cell types. We differentiated SH-SY5Y cells into mature neuron-like cells using a concise protocol and observed the upregulation of total APP throughout differentiation. However, APP transcript variant-1 was upregulated only during the early to middle stages of differentiation and declined in later stages. We identified the maturity state where this post-transcriptional shift occurs, terming it "true maturity." At this stage, we observed a predominant expression of mature neuronal and cholinergic markers, along with a distinct APP variant pattern. Our findings emphasize the necessity of using a differentiation state-sensitive marker system to precisely characterize SH-SY5Y differentiation. Moreover, this study offers an APP-guided, alternative neuronal marker system to enhance the accuracy of the conventional markers.
Aerobic exercise improves motor dysfunction in Parkinson's model mice via differential regulation of striatal medium spiny neuron
Authors: Wang Yinhao , Wei Longwei , Tan Mingli , Yang Zizheng , Gao Bo , Li Juan , Liu Yang , Zikereya Talifu , Shi Kaixuan , Chen Wei
Published: May 2024

The striatum plays a crucial role in providing input to the basal ganglia circuit and is implicated in the pathological process of Parkinson’s disease (PD). Disruption of the dynamic equilibrium in the basal ganglia loop can be attributed to the abnormal functioning of the medium spiny neurons (MSNs) within the striatum, potentially acting as a trigger for PD. Exercise has been shown to mitigate striatal neuronal dysfunction through neuroprotective and neurorestorative effects and to improve behavioral deficits in PD model mice. In addition, this effect is offset by the activation of MSNs expressing dopamine D2 receptors (D2-MSNs). In the current study, we investigated the underlying neurobiological mechanisms of this effect. Our findings indicated that exercise reduces the power spectral density of the beta-band in the striatum and decreases the overall firing frequency of MSNs, particularly in the case of striatal D2-MSNs. These observations were consistent with the results of molecular biology experiments, which revealed that aerobic training specifically enhanced the expression of striatal dopamine D2 receptors (D2R). Taken together, our results suggest that aerobic training aimed at upregulating striatal D2R expression to inhibit the functional activity of D2-MSNs represents a potential therapeutic strategy for the amelioration of motor dysfunction in PD.
Mechanism of complement inhibition by a mosquito protein revealed through cryo-EM
Authors: Andersen John F. , Lei Haotian , Strayer Ethan C. , Pham Van , Ribeiro José M. C.
Published: May 2024

Salivary complement inhibitors occur in many of the blood feeding arthropod species responsible for transmission of pathogens. During feeding, these inhibitors prevent the production of proinflammatory anaphylatoxins, which may interfere with feeding, and limit formation of the membrane attack complex which could damage arthropod gut tissues. Salivary inhibitors are, in many cases, novel proteins which may be pharmaceutically useful or display unusual mechanisms that could be exploited pharmaceutically. Albicin is a potent inhibitor of the alternative pathway of complement from the saliva of the malaria transmitting mosquito, Anopheles albimanus. Here we describe the cryo-EM structure of albicin bound to C3bBb, the alternative C3 convertase, a proteolytic complex that is responsible for cleavage of C3 and amplification of the complement response. Albicin is shown to induce dimerization of C3bBb, in a manner similar to the bacterial inhibitor SCIN, to form an inactive complex unable to bind the substrate C3. Size exclusion chromatography and structures determined after 30 minutes of incubation of C3b, factor B (FB), factor D (FD) and albicin indicate that FBb dissociates from the inhibited dimeric complex leaving a C3b-albicin dimeric complex which apparently decays more slowly.
HunStat – a simple and low-cost potentiostat for analytical and educational purposes
Authors: Vamos Istvan , Kertesz Vilmos
Published: May 2024

We have developed a truly low-cost (15 USD), simple do-it-yourself (DIY) potentiostat with compact dimensions.