bluuebunny/crossref_metadata_2025 huggingface dataset:
Rheological Characterization of Algal Suspensions for Bioethanol Processing
Authors: Cioroiu Doinita Roxana , Parvulescu Oana Cristina , Koncsag Claudia Irina , Dobre Tanase , Raducanu Cristian
Published: February 2020

The paper has aimed at studying the rheology of macroalgae aqueous suspensions in the presence of cellulase enzyme relevant to bioethanol processing by a subsequent fermentation. Rheological measurements of aqueous suspensions of Ceramium virgatum and Cladophora vagabunda macroalgae species were performed using a Couette geometry rotational viscosimeter. The effects of operation temperature (t=25, 50 �C), cellulase/dried algae ratio (R=0, 16 U/mgda), and algal suspension mass concentration (c=5-15%) on rheological behaviour and parameters were evaluated. Algal suspensions behaved as non-Newtonian fluids obeying either a Bingham plastic linear relationship or an Ostwald-de Waele power law corresponding to a pseudoplastic fluid. Characteristic dynamic viscosity of Bingham plastic fluids were in the range 0.045-0.115 Pa�s for C. virgatum suspensions and 0.021-0.114 Pa�s for C. vagabunda ones, whereas apparent viscosity varied from 0.138 Pa�s to 43.551 Pa�s for C. virgatum and from 0.181 Pa�s to 45.417 Pa�s for C. vagabunda. Data obtained in 8 rheological tests corresponding to a Bingham plastic behaviour of C. vagabunda suspensions, which were processed according to a 23 factorial experiment, emphasized an increase in suspension viscosity with all process factors. The results could be useful for optimization of enzymatic hydrolysis process in order to develop efficient and cost effective saccharification and fermentation strategies.
Influence of Hydroxypropyl Methylcellulose on Flowing and Swelling Parameters in Biomucoadhesive Tablets with Miconazole Nitrate
Authors: Birsan Magdalena , Bibire Nela , Vieriu Madalina , Panainte Alina Diana , Cojocaru Ileana
Published: February 2020

Original pharmaceutical formulations have been produced as oral biomucoadhesive tablets for antifungal medication. They have been obtained through direct compression using as matrix forming polymers various sorts of hydroxypropyl methylcellulose. The main goal of the study was determining the swelling index of the new mucobioadhesive formulations with miconazole nitrate in order to correctly evaluate the time of contact with mucosa, and the prolongation of drug release. For each formulation, the flowing parameters have been determined: flowing time, friction coefficient, repose angle, Haussner ratio, Carr index, and the swelling index for 6 formulations containing various sorts of hydroxypropyl methylcellulose as matrix molders, while the formulation variables studied were time and association ratio between those polymers. Though results analysis, we noticed that the values of the swelling index depended on the type and quantity of polymer, results that could also be explained by the proportionality relationship to flowing and compressibility parameters.
Determination of Traces Heavy Metals from Solid Residues Formed by Combustion of Lignite in Relation to Degree of Accessibility in Soil and Plants
Authors: Nica Badea Delia
Published: February 2020

The paper evaluates the presence and content of traces of heavy metals Hg, Pb, Ni, Cd (total forms) from coal and solid combustion products, the degree of transfer and accessibility in the area of influence of a lignite power plant. The content of toxic heavy metals in residues are characterized by RE Meiji [ 1 (Pb and Hg) and REMeij �1 (Ni and Cd) for the filter ash. Pb and Ni content in the soil exceeds normal values, and Pb exceeds and alert value for sensitive soils around the residue deposit (70.20 mg.Kg-1). The degree of accessibility of the metals in plants (TF), reported at the Khan reference value (0.5), indicates a significant bioaccumulation level for the metals: Cd (1.9) and Hg (0.6) inside the deposit; Cd (0.39) at the base of the deposit, Hg (0.8) in the area of the thermal power plant. The trace levels of heavy metals analyzed by GFAAS and CVAAS (Hg), indicates a moderate risk potential for food safety and quality of life in the studied area.
Chemical Reactions of Iodoform in the Study of Post-Extraction Alveolites
Authors: Budacu Cristian , Constantin Mihai , Chiscop Iulia , Stelea Carmen Gabriela , Dragomir Raluca
Published: February 2020

Post-operative alveolitis is a topical issue in dental practice, which is also reflected by the etiopathogenic aspects. The conservative principle requires the maintenance of dento-periodontal units in the arch for as long as possible, but there are situations where dental extraction is required. The healing process of the post-surgical wound is complex and involves processes of gingival mucosal regeneration and bone reshaping, involving several local factors: wound size, presence of infection, alveolar vascularization, intraalveolar foreign bodies, and general factors, especially general condition, age and body reactivity. The quality, structure, maintenance, and retraction of the clot are key factors in the formation of connective tissue during the healing of the post-extraction would. At the Oral and Maxillofacial Surgery Clinic of Gala�i, during a 2-year period between January 2015 and December 30, 2016, 2780 patients that required surgery - dental extraction were consulted and diagnosed. We found that among those 2780 patients with dental extractions 105 (3.77%) had post-treatment alveolitis. No post-surgical alveolitis from the case study was complicated by osteomyelitis of the jaws or by suppurations of the superficial or deep compartments of the face. The prophylactic measures in each dental extraction, together with the correct and timely curative treatment, combined with the dentist�s competence and responsibility, can shorten the time of suffering, actively combating the risk factor and accelerating the social reintegration of the patient with post-treatment alveolitis.
The Application of Enzymological Techniques for Determination of the Inhibitor Activity of Bio Enzym Extracted from Seawater Bacteria Pseudomonas aerogenosa to Fight Biocorrosion of Carbon Steel
Authors: Faiza Khoukhi , Kebouche Salima , Djelali Nacer Eddine , Gana Mohamed Lamine
Published: February 2020

The paper presents a studies for the development of new corrosion inhibitors by biotechnological way applicable to many areas of petroleum industry including, characterization of protective films on carbon steel and biocorrosion inhibition. Our research is oriented towards the isolation of new strains of bacteria from the sea waters of the Mediterranean region that have power (inhibitory and/or bactericidal that blocks the growth of sulfate-reducing bacteria responsible for microbiologically influenced by production of Pyocyanin (1 hydroxyphenazin) or 5-methylphenazin-1-one. Our first objective was to isolate the population of Pseudomonas Aerugenosa on a specific medium, characterization gram, then fermentation in a nutrient broth. The second objective of this study is evaluation of corrosion rate by weight loss method with injecting different dose of the crude enzyme extract CEE containing the methylphenazine (Pyocyanin). An efficacy test was performed on test kit vials containing the specific culture medium with the SRB contaminated with industrial injection water from SONAHESS region. The total disappearance of the SRB is 40% of CEE by blocking their metabolism in the growth phase confirms the efficiency of treatment.
Identification of Bacteria Involved in Periodontal Disease Using Molecular Biology Techniques
Authors: Martu Ioana , Goriuc Ancuta , Martu Maria Alexandra , Vata Ioana , Baciu Raluca , Mocanu Raluca , Surdu Amelia Elena , Popa Cosmin , Luchian Ionut
Published: February 2020

Periodontal disease is progressive and episodic in nature, with tissue destruction resulting from the host response to bacterial antigens and irritants.This study aimed to evaluate the genotype of a certain group of pathogen agents. In order to to this we used a total of 45 patients with PAG included in the epidemiological analyzes with a mean age of 34.7 Four samples were collected from the subgingival plaque from each patient, a total of 224 samples of dental plaques were investigated. All samples were tested for the presence of Tannerella forsythensis, Porphyromonas gingivalis and Treponema denticola. We found that patients had a large number of pockets colonized by Tannerella forsythensis gingivalis (88.6%) Porphyromonas gingivalis (59%) and Aggregatibacter actinomycetemcomitans (25%). The data of this study support the evidence Tannerella forsythensis as a periodontopatogen and confirmed a strong association between Porphyromonas gingivalis and Tannerella forsythensis in aggressive periodontitis and support previous findings that generalized aggressive periodontitis are associated with more complex microbiota.
Biomarkers in Diagnosing Preeclampsia and Their Correlation with Blood Pressure
Authors: Mladin Micoara Narcisa Carmen , Lungeanu Diana , Morariu Stelian Ioan , Ciacli Camelia Anisoara , Moleriu Lavinia Cristina , Toth George , Stelea Lavinia , Petre Izabella , Chiriac Dana , Ionita Ioana , Puschita Maria
Published: February 2020

Together with their general condition, the ratio of sFlt-1 / PlGF is an objective factor in the assessment of suspected preeclampsia. Recent research has shown that the ratio sFlt-1 / PlGF can be associated with the severity of preeclampsia, providing a short-term prediction about pregnancy. For this follow up study we considered a sample of 30 pregnant woman�s all of them having tension problems. Our results confirm a decreased PlGF serum in patients with preeclampsia.
Obtaining the Volatile Oils from Wormwood and Tarragon Plants by a New Microwave Hydrodistillation Method
Authors: Mosteanu Danut , Barsan Ghita , Otrisal Pavel , Giurgiu Luminita , Oancea Romana
Published: February 2020

In the present paper we describe an original installation for microwave hydrodistillation at low pressure that we constructed in order to obtain volatile oils from different plants. The results that we have obtained are encouraging meaning that the main components of these oils were found in correspondence with the data presented in literature. A novelty of this process is the fact that the hydrodistillation time was shorter than in the case of a classic process of hydrodistillation at atmospheric pressure. The aim of our research was to find a new and modern way of obtaining volatile oils from plants.
Effectiveness of Nickel-Titanium Endodontic Instruments on Smear Layer Removal from Radicular Dentin. A Scanning Electron Microscopy Study
Authors: Monea Monica , Biris Carmen , Bechir Edwin Sever , Suciu Nicoleta , Pop Anca Maria , Olah Peter
Published: February 2020

The aim of our study was to evaluate the cleaning effect of nickel-titanium (Ni-Ti) rotary files on the smear layer formed on the radicular dentine walls during endodontic procedures. We used 50 freshly extracted maxillary permanent teeth divided in two study groups according to the instruments used for cleaning: Group 1 with ProTaper Ni-Ti rotary files, Group 2 with manual Ni-Ti files. Longitudinal sections of root canals from each group were evaluated by scanning electron microscopy (SEM) in the apical, middle and coronal sections. Representative microphotographs were taken and double blind scoring was performed by calibrated examiners. The amount of smear layer was measured according to Hulsmann scoring system and the data were statistically analyzed. There were statistically significant differences between instruments in the apical root canal sections (p[0.05), where the manual Ni-Ti files gave the best cleaning scores. There were no differences between groups for the middle and coronal thirds. In conclusion, the best approach for an effective removal of smear layer is to combine manual files with rotary Ni-Ti instruments during the endodontic treatment.
Self-assembled Nanomaterials Type Layered Double Hydroxides Antibiotics Used as Drug Delivery Vectors
Authors: Duceac Letitia Doina , Stafie Liviu , Banu Elena Ariela , Paduraru Oana , Calin Gabriela , Ciuhodaru Madalina Irina
Published: February 2020

Due to their biocompatibility, nanomaterials type layered double hydroxides (LDHs) development has attracted many researchers attention especially in medical area using them as drug delivery vectors. Their efficiency is due to the drug controlled release from the interlayer space of LDHs matrices. The controlled drug delivery characteristic is due to the reduction of drug concentration variations and maintains the desired level of drug concentration for long time. Furthermore, this action limits adverse effects and reduces the therapy duration, enhancing bactericidal activity and leading to an effective treatment. This work highlights the synthesis and characterization of hybrid structures type LDHs- tobramycin having application as antibacterial factor against Pseudomonas aeruginosa. The obtained self-assembled structures were morphologically characterized in order to point out the drug-interlayered products. Results indicate superior properties of MgAlLDHs and ZnAlLDHs behaving as host for a wide variety of molecules generally and, in our case, for tobramycin as guest.
Biochemical Modifications in HIV-Infected Children and Adolescents Under Antiretroviral Therapy
Authors: Jugulete Gheorghita , Iacob Simona , Merisescu Madalina , Luminos Monica
Published: February 2020

In this paper we propose to present the biochemical modifications in children and adolescents with HIV infection/AIDS undergoing antiretroviral therapy (ART). We have carried out a retrospective study on 200 HIV-seropositive children registered in our department, without any prior workup modifications, in which antiretroviral therapy was initiated. In all patients we have monitored serum glucose, amylasemia, natremia, and liver enzymes. Most of the biochemical modifications were mild and moderate and did not necessitate cessation of therapy. In only 2 cases, modifications were severe enough to impose a temporary interruption of the ART: one case with elevated triglycerides and one case with elevated liver enzymes. After 24 months of ART, approximately 11.5% (23/200) of the monitored patients had biochemical modifications: 3.5 % elevated triglycerides, 5% elevated liver enzymes, 2.5 % hyperamylasemia, and 0.5 % hypernatremia. Biochemical modifications were more significant in patients who received prior antiretroviral therapy, particularly those who received protease inhibitors (PIs) in comparison to patients naive to ART.
Rural and Urban Management of Toxic Aluminum Waste in Relation with Environmental Protection in Iasi County
Authors: Costuleanu Carmen Luiza , Ignat Gabriela , Brezuleanu Olguta , Ungureanu George , Robu Dragos , Vintu Catalin Razvan , Boghita Eduard , Brezuleanu Stejarel
Published: February 2020

Aluminum in excess could cause diseases, affecting bones, brain, liver, heart, spleen, as well as muscles. Aluminum in higher concentrations is found in tap water since aluminum compounds are used for water treatment before being supplied. Furthermore, aluminum foils and coatings are used in the processes of cans and boxes packaging, especially for foods and beverages, taking into account its capacities as efficient barrier. The aim of the present studies was represented by the comparison of aluminum packaging waste generation in two areas from Iasi county, a rural and an urban one. Meanwhile, we compared the ratio of aluminum packaging waste and total packaging waste generation, as well as the ratio of aluminum packaging waste and total waste generation in the studied areas. We found a statistical significant difference of 71.31 % (as average) between the aluminum packaging waste quantities in urban and rural zones. Aluminum packaging waste was considered to be any packaging coated by or including aluminum. We were not able to differentiate the aluminum foil wrap from other parts of packaging. Moreover, it seems to exist a small difference of the ratios of aluminum packaging waste and total packaging waste generation in urban area and rural one, but not statistically significant. The selective recycling is almost impossible in rural zones, the lack of education and of means representing the major challenges. The difficulties in managing the waste having as an important component the aluminum packaging could increase the risks for human health.
Treatment Methods Conditioned by the Gravity of Drug-Induced Gingival Hyperplasias
Authors: Calin Alina Mihaela , Debita Mihaela , Dragomir Raluca , Stefanescu Ovidiu Mihail , Budacu Cristian , Szalontay Andreea Silvana
Published: February 2020

The first drug discovered to be involved in the development of gingival hyperplasia is phenytoin, which is indicated in the treatment of epileptic patients. The other drugs are calcium channel blockers with vasodilating effect. The most important one is Nifedipine, while Ciclosporin A, which is used as an immunosuppressant in the prevention of transplant rejection, causes gingival hyperplasia as a secondary effect. Gingival hyperplasia can reach an impressive volume, completely covering the dental crown and affecting the masticatory and physiognomic functions. The elucidation of the mechanism, by which drug-induced gingival hyperplasia occurs, favoring factors and the choice of conservative or surgical treatment methods, emphasizing the prophylactic treatment. The study batch was subject to intraoral and extraoral clinical examinations and the data were included in the dental treatment sheet of each patient, 11 patients aged over 60 years, who came to the Clinic ... in the period 2014-2016. The diagnosis was based on the anamnesis, the clinical aspect of the lesions and the histopathological examination. After the surgical excision of the hyperplasia affected area, recurrence was prevented by dispensarizing the patients and controlling the bacterial plaque through rigorous oral hygiene. Treatment depends on the severity of the lesions, as well as on the physionomic and masticatory functions. Conservative etiological therapy is attempted, by removing the bacterial plaque and local irritant factors, by reducing the dose of drugs, or by changing the systemic medication.
Preparation and Characterization of Ultrafiltration TiO2 Nanoparticles-Polysulfone Membranes
Authors: Enache Daniela Florentina , Popa George Alexandru , Vasile Eugeniu , Razvan Anca , Oprea Ovidiu , Voicila Elena , Dumitru Florina
Published: February 2020

This paper presents the preparation route for new TiO2 nanoparticles-polysulfone membranes: M1 (Psf 12%), M2 (Psf 12% + TiO2 anatase), and M3 (Psf 12% + TiO2 76% anatase+ 24% rutile) that were structurally characterized by FTIR, TG-DSC and by scanning electron microscopy (SEM) coupled with energy dispersive spectroscopy (EDAX). Contact angle measurements, dead-end and cross-flow filtration experiments were carried out to characterize the morphology and hydrodynamic performance of the prepared membranes. Improved mechanical properties, enhanced hydrophilicity and the relative large water flux measured for M2-M3 (721.83 L/m2�h and 305.4 L/m2�h, respectively) in cross-flow filtration experiments, make these membranes appropriate for ultrafiltration applications.
PCR Analysis of the Herpesviruses Presence in Crevicular Fluid in HIV- Positive Patients
Authors: Popa Cristina , Stelea Carmen Gabriela , Filioreanu Ana Maria , Sufaru Irina Georgeta , Maftei George Alexandru , Arbune Manuela , Martu Silvia , Popescu Eugenia
Published: February 2020

The present study aimed to determine the frequency of herpesviruses in gingival fluid samples in patients with periodontitis HIV compared to HIV-negative subjects. Gingival crevicular fluid samples were obtained from 28 patients with HIV-positive periodontitis and from 14 patients with HIV seronegative periodontitis. Herpesviruses have been identified by PCR amplification methods. In HIV-positive patients, the most prevalent herpes virus was HCMV, followed by HHV-6 and HHV-7. In non-HIV-related periodontitis, HCMV was identified in 11 samples and EBV-1 in 8 samples, followed by HSV (7 samples). HIV seropositive samples showed an average of 4.0 herpesviruses and HIV-seronegative individuals averaging 1.4 herpesviruses. EBV-2 and HHV-8 were detected exclusively in subgingival samples from HIV-positive patients. HIV-induced activation of herpes viruses may be a stimulating factor for rapid periodontal destruction. Patients with severe immunosuppression may experience herpesvirus-mediated gingival necrosis. The hypothesis that HIV periodontitis is the result of a combined infection of herpesviruses and bacterial pathogens should be studied further.
The Role of the Immunohistochemical Examination in the Diagnosis of Castleman`s Disease
Authors: Badulescu Oana Viola , Hultoana Roxana , Ciuntu Bogdan Mihnea , Papancea Adelina , Georgescu Stefan Octavian
Published: February 2020

Castleman�s disease is characterized by benign angiolymphoid hyperplasia. There are two clinical forms: the localized form in which one lymph node is affected, often paucisimptomatic, treated by radical surgical excision, without recurrence in most cases, and the multicentric form, characterized by systemic manifestations, polyadenopathy (mediastin, cervical region, abdomen, etc.) , organomegaly (e.g. spleen, liver), with a less favorable prognosis than the localized form. The etiology of the disease remains unknown; recent studies suggest the implication of human herpes virus 8 (HHV-8), especially in multicenter forms. We shall present the case of women, aged 23, with Castleman�s disease with retroperitoneal localization, who was admitted to the surgery clinic due to abdominal pains. The initial abdominal CT raised the suspicion of a hepatic lobus caudatus tumor, but the anatomical-pathological result confirmed the diagnosis of angiofolicular hyperplasia of the lymph nodes. Diagnostic methods, histological forms, treatment and prognosis aspects of Castleman�s disease are described below.
Negative Pressure Therapy in Wounds Surgical Treatment
Authors: Ciuntu Bogdan Mihnea , Georgescu Stefan Octavian , Cirdeiu Ciprian , Timofte Daniel , Azoicai Doina , Tecuceanu Angela , Moraru Dan Cristian , Schaas Carmina , Blaj Mihaelau , Tamas Camelia
Published: February 2020

The study aims to assess the significance of negative pressure therapy in the treatment of 1 January 2014 - 31 June 2017. The objectives intend to evaluate the healing time required after applying the method and the functional consequences for the patient. A prospective study was conducted on a sample of 31 patients with various tipe of wounds which were monitored their clinical course between September 2014 - February 2017, following negative pressure therapy. There were used vacuum assisted closure devices (VAC � -Hartman) in order to apply negative pressure to the wound, while complying with specified settings in accordance with patients� outcome. Healing was obtained in all cases, to an average hospital stay of 30 days and 12 days of therapy application.The negative result of microbial cultures was obtained after an average of 7.55 days by simultaneous application of negative pressure and antibiotic treatment according to the antibiogram. After basic treatment of the wound, auxiliary methods such as negative pressure contribute to the healing. Evolution was favorable with wound granulation in 95% cases, which allowed surgery under local anesthesia, and defect was covered with skin graft. VAC therapy falls into the last group of treatments by eliminating healing inhibitors. This regenerates the wound in a damp environment and essentially turns an open wound into a closed system.
Preventive andTherapeutic Effects of Chlorhexidine Containing Varnish on Candida Biofilm
Authors: Mensch Karoly , Pongracz Julia , Nagy Adam , Kristof Katalin , Bechir Anamaria , Pacurar Mariana , Nagy Gabor
Published: February 2020

The aim of the study was to assess the preventive and therapeutic efficacy of a sustained-release varnish (SRV) Cervitec Plus� compared with Corsodyl� mouthwash against Candida albicans, Candida parapsilosis, Candida tropicalis and Candida glabrata on acrylate surfaces. Nystatin as the positive control, and sterile saline solution as the negative control, were used. To examine the preventive efficacy of the agents, Candida biofilms were developed in Sabouraud liquid medium, and mature biofilms were treated with the antifungal. To investigate the preventive efficacy, the agents were applied to the wells of the plates and the biofilms were grown in the treated wells. To determine the metabolic activity of biofilms, the XTT-assay was used. In the case of C. albicans and C. parapsilosis, the most effective therapeutic product was Corsodyl� followed by Cervitec Plus� and Nystatin. The differences were statistically significant when Corsodyl� was compared to Nystatin ( p � 0.05). In relation to C. tropicalis and C. glabrata, Corsodyl� showed the greatest therapeutic efficacy followed by Nystatin and Cervitec Plus�. For both species, the results of Corsodyl� and Cervitec Plus� showed statistically significant difference ( p � 0.05). The most effective preventive product for each examined Candida species was Nystatin, followed by Cervitec Plus� and Corsodyl�. Significant differences were between Nystatin and Corsodyl� (p � 0.05). The preventive and therapeutic efficacy of Cervitec Plus� and Corsodyl�, against the examined Candida species, was detected. Because of the disadvantageous side-effects of Nystatin, Cervitec Plus� can be considered as an alternative preventive agent against Candida species.
Influence of Weight of Pregnant Women on First Trimester Biochemical Markers Values
Authors: Navolan Dan , Ionescu Cringu Antoniu , Carabineanu Adrian , Birsasteanu Florin , Cretu Octavian , Szasz Florin , Vladareanu Simona , Ciohat Ioana , Gidea Ramona , Nemescu Dragos , Farcas Simona , Andreescu Nicoleta , Simu Sebastian , Stoian Dana
Published: February 2020

Values of first trimester biochemical markers (PAPP-A and free b-hCG) concentration are included in aneuploidies risk evaluation algorithm. Since both markers are produced by the fetus and placenta their concentration depends on the volume in which they are dissolved, respectively the weight of the pregnant women. Our study aimed to analyze the influence of maternal weight on first trimester biochemical markers concentration and the ability of the risk calculation software to correct this influence. Pregnancy-associated protein A (PAPP-A) and free � chorionic gonadotropin hormone (free � hCG) first trimester sera concentration respectively weight were measured in 1629 pregnant women. First trimester PAPP-A and free beta hCG concentrations inverse correlate with weight of pregnant women rho=-0.33, p[0.0001, respectively rho=-0.18, p[0.0001. Weight of pregnant women inversely correlates with multiple of median (MoM) values of first trimester markers too: rho=-0.38, p[0.0001 (PAPP-A), respectively rho=-0.17, p[0.0001 (free-b-hCG). The software counterbalances the influence of weight on biochemical markers values. PAPP-A corrected MoM (MoMc) values don�t inversely correlate with the weight (rho=-0.03, p=0.12), whereas free � hCG MoMc values showed an extremely weak inverse correlation (rho=-0.08, p=0.0008). The software counterbalances the influence of weight on PAPP-A values, whereas an extremely weak but insignificant inverse correlation between weight and free-beta hCG values persists after correction.
Comparative Study Regarding Heavy Metals Content in Air from Targu Jiu and Rovinari
Authors: Capatina Camelia , Cirtin Daniela
Published: February 2020

Environmental pollution represents an important and complex subject with a huge concern for both scientists in different fields, but also for the governments, national and international organizations. Besides the indisputable advantages of the industry for the human development, it has a big contribution to environmental pollution with negative effects to the human health. Heavy metals in the environment have negative effects to the ecosystems and to human health. Consequently, it is necessary to determine the level of heavy metals in the environment to take measures to reduce the environment pollution. According to this issue, the main purpose of these research studies is to perform a comparative analysis of heavy metals content in Targu-Jiu and Rovinari area, those being ones of the most pollution affected areas in Jiu county. The results obtained show that the heavy metals contents in PM10 in the air in these areas chosen for the study are below the permitted limit values for the period 2015-2016.
Cavitation Erosion Behaviour of Cooper Base Layers Deposited by HVOF Thermal Spraying
Authors: Bordeasu Ilare , Mitelea Ion , Lazar Iosif , Micu Lavinia Madalina , Karancsi Olimpiu
Published: February 2020

The recent development in enhancing the corrosion resistance of materials by covering with powdered layers open the way to use the procedure also for cavitation erosion protection, phenomenon always present in hydraulic machinery runners as well as ship propellers. The present research analyzes the behavior of four different types copper layers, deposited with high velocity flames, HVOF upon specimens realized from cast steel for general use 270-480 W (equivalent with OT500-3 used in Romania), to cavitation erosion in a Laboratory device. Even if the powder density and the layers thickness have close values, the behavior to cavitation erosion is different and depend primarily on the powder chemical composition and the microscopic structure.
Nanoparticles Deposition on Mini-implants for Osseo-integration and Antibacterial Properties Improvement
Authors: Zegan Georgeta , Carausu Elena Mihaela , Golovcencu Loredana , Sodor Botezatu Alina , Cernei Eduard Radu , Anistoroaei Daniela
Published: February 2020

In last few years, researchers focused on improvement of lifespan of dental mini-implants by coating them with nanopoarticles for a better osseo-integration, which also possesses antibacterial properties. Once their surface is coated with nanoparticles type silver dopped hydroxyapatite makes them durable and safe by a good integration into tissue and by preventing bacterial infections. It is well known that a particular problem with medical devices such as dental mini-implants is bacterial colonization. For this reason the use of nanoparticles coated mini-implants is a measure to prevent or to treat infections caused by microbial agents present in oral cavity. Our study considers the possibility of using silver doped hydroxyapatite thin films on titanium mini-implants as an osseo-integration and antibacterial component. Therefore several physic-chemical analyses were performed in order to determine their properties and to open new perspectives to develop nanoparticulated thin films as coatings for orthodontic mini-implants.
The Role of Low Molecular Weight Heparin in Pregnancies of Patients with Inherited Thrombophilia that Have Presented (and) Thrombotic Complications During Previous Pregnancies
Authors: Mitranovici Melinda Ildiko , Puscasiu Lucian , Craina Marius , Iacob Daniela , Chiriac Veronica Daniela , Ionita Ioana , Moleriu Radu Dumitru , Furau Gheorghe , Sisu Alina , Petre Izabella
Published: February 2020

What is presently known is that thromboembolic disease represents a major cause of morbidity and mortality during pregnancy. To this we add a rare but not negligible pathology, acquired and inherited thrombophilia, which increases the predisposition for thrombotic events during pregnancy. In this article, what has been studied is the impact that LMWH has on patients with inherited thrombophilia, both the conventional FV, FII and the newly introduced MTHFR, PAI, FXII, factors which are often combined.81 patients have been taken into consideration / included in study, with the following anterior results, before the patients discovered their illnesses, thus before following treatment: first trimester or advanced pregnancy loss, IUGR, premature birth or secondary placental thrombotic complications such as: preeclampsia, DPPNI or dead fetuses, as well as maternal thrombotic accidents. The administration of LMWH decreased the rate of pregnancy loss compared to untreated pregnancies is statistical significant (p= 0.004), there were fewer DPPNI (p= 0.006), and no intrauterine deaths. We also compared the occurrence of thrombotic events during treated and untreated pregnancies and we observed significant differences in this groups (p= 0.001). There was also a sufficient number of patients with new types of thrombophilia included in the study to demonstrate the impact it has on pregnancy. LMWH improves the situation of women with thrombophilia during pregnancy.
Assessment the Activity of Some Enzymes and Antibiotic Substances Sensitivity on Pathogenic Bacteria Species
Authors: Sarbu Ionela , Vassu Tatiana , Chifiriuc Mariana Carmen , Bucur Marcela , Stoica Ileana , Stefana Petrut , Rusu Elena , Moldovan Horatiu , Pelinescu Diana
Published: February 2020

Microbial and opportunistic pathogens cause serious problem to human host by releasing different compounds which are involved in colonization, invasion or in alteration of immune process. In this study we propose ourselves to analyze the virulence profile of microorganisms involved in urinary tract infections and bacterial vaginosis in order to compare the virulence mechanisms developed by these microorganisms. We assessed the virulence profile (motility, adhesion to HeLa cell line, biofilm formation, production of enzymes, antibiotic susceptibility) using phenotypic methods. The microbial strains isolated from urinary tract infections present a high ability to release amylases, caseinase, siderophore-like, half of them have present high motility and also were highly resistant to antibiotics, while the microbial strains isolated from bacterial vaginosis present a high ability to bind human epithelial cells and to release hemolyzin and DN-ase.
Studies on the Correlation Between Biosurfactant and Lipid Synthesis in Candida tropicalis CMGB114 Using Hydrocarbons and Vegetable Oil Wastes
Authors: Csutak Ortansa , Corbu Viorica , Vassu Tatiana
Published: February 2020

During past decades there is an augmented interest regarding the possibility of biosurfactant and lipid production using microorganisms able to assimilate various polluting substrates represented by hydrocarbons and industrial or household wastes such as glycerol and fried vegetable oil. The yeast strain Candida tropicalis CMGB114 produced hhigh rates of biosurfactants when grown on yeast extract and peptone supplemented with 1% petroleum, fried sunflower oil or n-hexadecane, respectively, on yeast extract and NH4NO3 with 40 g/L glycerol. Proliferating lipid bodies were observed in the cells from all the tested culture media, indicating an active lipogenesis and a correlation between biosurfactant and lipid synthesis. Future research will focus on developing strategies for practical applications of C. tropicalis CMGB114 in bioremediation and biofuel production.
Detection and Assessment of Interleukin 6 in Irreversible Pulp Inflamation
Authors: Giuroiu Cristian Levente , Vataman Maria , Melian Gabriel , Bularda Dragos , Lozneanu Ludmila , Salceanu Mihaela , Patrascu Alexandru , Andrian Sorin , Melian Anca
Published: February 2020

The study aimed to assess the number, localization and distribution of interleukin 6 (IL-6) positive cells in healthy pulp, acute and chronic pulpitis.
The study group included 48 patients aged between 18-72, treated in University of Medicine and Pharmacy Grigore T. Popa Iasi, Romania. The pulpectomy was performed on 42 patients diagnosed with acute and chronic pulpitis. The other 6 patients, without signs of dental caries or periodontal disease, were submitted to extractions of teeth for orthodontic purposes, with pulpectomy performed before extraction. The pulp samples were examined with optic microscope. The detection and assessment of IL-6 were performed using immunohistochemical technique. Data were statistically analysed using non-parametric tests. According to morphopathological criteria, 42.85% were classified as acute pulpitis and 57.14% as chronic pulpitis. The pulp samples in control group were not associated with IL-6 positive cells. The analysis of all samples with acute and chronic pulpitis identified 73.80% samples with IL-6 and 26.20% associated with the absence of IL-6. The highest frequency of IL-6 positive cells was recorded in rich-cell zone of crown dental pulp. The systemic distribution of IL-6 positive cells was mostly diffused without well-defined orientation. IL-6 release in acute and chronic pulpitis is significantly higher comparing with healthy pulp tissue.
Studies on Obtaining and Characterization a Pregabalin-cyclodextrin Complex for Taste Masking Purpose
Authors: Popa Gratiela , Dragostin Oana , Buzia Olimpia Dumitriu , Tartau Liliana Mititelu , Profire Lenuta , Gafitanu Carmen
Published: February 2020

Pregabalin is an anticonvulsant and analgesic drug that stirs more and more the interest of the researchers. Its stability, solubility and taste can be modified by obtaining complexes with cyclodextrins. For this purpose, in the present study betacyclodextrin and hydroxypropyl-betacyclodextrin were included. The obtaining of inclusion complexes was confirmed by ATR-FTIR spectroscopy and UV spectroscopy. The results demonstrated that it is possible to develop inclusion complexes of pregabalin with betacyclodextrin and hydroxypropyl-betacyclodextrin, with moderate efficiency.
Synthesis and Characterization of Nanosized Copper(II)-Phthalocyanine Pigment (PBI 15:3) with the Modified Surface. I
Authors: Athanasiu Angela Anca , Deaconu Marian , Crudu Marian , Doncea Sanda , Stoica Rusandica , Oproiu Loti , Senin Raluca , Ruse Mircea , Rosu Dan , Filipescu Catalin
Published: February 2020

The research activity presented in this paper is intended to investigating of certain valuable nanosized blue pigment ( P Bl 15:3) prepared by 3-way synthesis (functionalization reactions) and conditionin(dispersing with or without encapsulation), namely obtaining stable aqueous dispersions of blue pigment with modified surface used in medium nonspecific for pigments. The techniques employed to characterize the structure and properties of blue pigment dispersions with functionalized particles compared to the original powder product were characterized by Fourier transform infrared spectroscopy FT-IR, thermogravimetric analysis (TGA), elemental analysis (EA) and particle size distribution (the distribution and average of particle diameter).In our finding the nano-dispersions obtained contain functionalized blue pigment particles with or without encapsulation in the form of liposomes with the average diameter below 200-300 nm, representing good results.
Smart Hydrogels with Collagen Structure Made of Pelt Waste
Authors: Zainescu Gabriel , Constantinescu Rodica Roxana , Sirbu Carmen
Published: February 2020

The area of interest is the synthesis and study of properties of new types of hydrogels made from pelt waste, in order to recover waste from tanneries. Leather processing in tanneries results in about 500-600 kg of pelt waste from a ton of raw hides. These hydrogels are made using smart processes in order to then be applied in agriculture, for preservation of water in the soil or for controlled release of fertilizers, pesticides but also for the development of additivated agricultural film biodegradable over time (between 1 month and 6 months). Hydrogels that are based on biopolymers, compared with hydrogels based on synthetic polymers, have the advantage of biodegradability, biocompatibility, and a low level of toxicity. The paper presents the production of biodegradable polymer mixtures obtained from hydrolysis and enrichment of the resulting hydrolysate with phosphorus and potassium. Hydrogels with collagenous structure are tested using a high-performance instrumental analysis system (FT-IR-ATR, UV-Vis-NIR, SEM, EDAX, etc). The paper presents an experimental model for obtaining hydrogels with collagenous structure from pelt waste resulting from the liming process.
Dietary Supplements with Resveratrol, Flavonoids and Phenolic Acids: in-depth HPLC Profiling and Antioxidant Capacity as Quality Markers
Authors: Ardelean Florina , Vlase Laurian , Mocan Andrei Marius , Gheldiu Ana Maria , Antal Diana Simona , Trandafirescu Cristina , Marginean Otilia , Dragan Simona
Published: February 2020

Dietary supplements containing natural compounds, including resveratrol, are increasingly used as health improving products. The present study had three objectives, namely: the quantification of resveratrol by HPLC-MS in nine dietary supplements, the qualitative and quantitative assessment of 18 phenolic acids and flavonoids in the same supplements, and finally the measurement of the antioxidant capacity of these products. The content of resveratrol in commercial samples ranged between 22.8 and 104.7 % of the declared amount. The highest number of polyphenols that could be quantified in a dietary supplement was nine. The values obtained for antioxidant capacity suggest that resveratrol supplements containing below 10 mg of the stilbene have, even in the presence of other components, a very low antioxidant capacity which may be insufficient for an attainment of the desired antioxidant status. To our knowledge, this is the first HPLC-analysis cumulating the quantification of resveratrol with that of eighteen other polyphenols in dietary supplements, giving an in-depth overview of their composition and health benefits from the viewpoint of antioxidant capacity.
Determinants of Hemoglobine Value in Pregnant Women: Results from a Cross-Sectional Study
Authors: Botnariu Gina , Popa Alina , Toma Vasilica
Published: February 2020

Anemia is a public health problem, being associated with high maternal and fetal morbidity and mortality. Identification of favorable factors in population level is extremely important. We assessed in 400 pregnant women, hospitalized in Maternity Cuza-Voda Iasi, the relationship between sociodemographic, dietary and educational factors and degree of anemia, by using a food frequency questionnaire and a nutritional knowledge questionnaire. The prevalence of anemia in the study group was 20.05%. After multiple regression analysis (made step by) there was shown that anemia is significantly correlated with total iron and calcium intake, total cholesterol and nutritional knowledge.In our group, anemia had a significant incidence, being related with common factors such as iron intake (from food and supplements) and nutritional knowledge. These data highlight the importance of counseling and education in pregnant women in anemia prevention.
Iono-molecular Separation with Composite Membranes III. Nitrophenols separation on polysulphone and composite nanoparticles ultrafiltration
Authors: Ani Hussam Nadum Abdalraheem Al , Cimbru Anca Maria , Tanczos Szidonia Katalin , Din Ion Spiridon , Cuciureanu Adriana , Nafliu Ion Marius , Nechifor Gheorghe
Published: February 2020

Building on the excellent results of extraction on a solid phase (polymer) this work combine adsorption nitrophenols (a, m, p-nitrophenols) on polymeric nanoparticles (NP-PSf) and composite (NP-PSf-PANI) with colloidal ultrafiltration. Colloidal ultrafiltration solutions of nitrophenols is done in an ultrafiltration plant, CELF System, with a capacity of 500 mL at 25oC, variable working pressure (1-10 atmospheres), turbulent flow regime (2-4 m/s) and usable filter polysulfone-membrane composites 10% in dimethylformamide coagulated with methanol polyaniline (PANI-PSf) 15 cm2. Operational parameters of the process: concentration nitrophenols, nanoparticle concentration, pH of the feed solution were studied, trying to correlate the results of the colloidal ultrafiltration with the nature of the nanoparticles. Permeate flow is optimal at a pressure of 5 atm and nitrophenols retention depends on their nature. Good results have been obtained at pH = 3.1 for polysulfone nanoparticles (NP) and excellent retention at pH 1.3 or pH]7, for composite nanoparticles (NP-PSf-PANI). Rejection o-nitrophenols is superior to the others two nitrophenols in all experiments.
The Dynamics of Salivary Parameters in Patients Undergoing Orthodontic Treatment
Authors: Buzatu Roxana , Gotia Smaranda Laura , Valceanu Anca S. , Scrobota Ioana , Onisei Doina , Szuhanek Camelia
Published: February 2020

From assisting in digestion and lubricating soft tissues to neutralizing plaque and promoting tooth remineralization, saliva is crucial to the oral environment. Our objective was to analyze the dynamics of salivary parameters in patients with orthodontic treatment. Subjects were divided into two age-related groups. Results showed an increase in the number of epithelial cells and leukocytes, as well as various changes in glutathione and C-reactive protein levels. Another significant finding was that young people were more prone to developing oral candidiasis after receiving a fixed orthodontic appliance.
Determination of Phenicols Using a Fast and Reliable HPLC Method Developed on Hypercarb Stationary Phase
Authors: Stan Georgeta Simona , Moldovanu Florentina , Badea Irinel Adriana
Published: February 2020

Hypercarb porous graphitic stationary phase was used to develop a fast and reliable method for simultaneous determination of chloramphenicol, thiamphenicol and florfenicol. The separation was achieved in 7 min elution being made isocratic using water modified with acetonitrile as mobile phase. The method was fully validated and showed good linearity, precision and accuracy. Limit of detection and quantification together with decision limit and detection capability were established. This method was applied for analysis of milk samples.
Heat Degradation Kinetics of the Chlorophyll from Spinach and its Correlation with the Reflection Spectra
Authors: Popescu Elena Corina , Buruleanu Claudia Lavinia
Published: February 2020

Chlorophyll, the pigment responsible for the green color specific to the spinach leaves, is sensitive to temperature, pH, activity of enzymes. The heat degradation kinetics of the chlorophyll a and chlorophyll b from the spinach leaves was studied at different temperatures (60, 70, 80 and 900C) and different times (3, 9, 12, 15 min). The degradation of the chlorophyll a and chlorophyll b followed a first-order reaction, and the rate constant had values between 0.0199-0.0695 min-1 for chlorophyll a and 0042 -0.011 min-1 for chlorophyll b respectively. The dependence between the chlorophyll degradation and temperature was modeled using the Arrhenius�a equation. The activation energy was by 44.316 Kjmol-1 for chlorophyll a and 35.384 Kjmol-1 for chlorophyll b respectively. Using the rapid, non-invasive measuring method of the reflection spectra in red band and in NIR region and the calculation of the index Clred edge, the chlorophyll quantity from spinach leaves was measured with a relative error ErCle between 1.27 and 8.65%.