bluuebunny/crossref_metadata_2025 huggingface dataset:
Weak Capacitance Detection Circuit of Micro-Hemispherical Gyroscope Based on Common-Mode Feedback Fusion Modulation and Demodulation
Authors: Zhang Xiaoyang , Li Pinghua , Zhuang Xuye , Sheng Yunlong , Liu Jinghao , Gao Zhongfeng , Yu Zhiyu
Published: May 2023

As an effective capacitance signal produced by a micro-hemisphere gyro is usually below the pF level, and the capacitance reading process is susceptible to parasitic capacitance and environmental noise, it is highly difficult to acquire an effective capacitance signal. Reducing and suppressing noise in the gyro capacitance detection circuit is a key means to improve the performance of detecting the weak capacitance generated by MEMS gyros. In this paper, we propose a novel capacitance detection circuit, where three different means are utilized to achieve noise reduction. Firstly, the input common-mode feedback is applied to the circuit to solve the input common-mode voltage drift caused by both parasitic capacitance and gain capacitance. Secondly, a low-noise, high-gain amplifier is used to reduce the equivalent input noise. Thirdly, the modulator–demodulator and filter are introduced to the proposed circuit to effectively mitigate the side effects of noise; thus, the accuracy of capacitance detection can be further improved. The experimental results show that with the input voltage of 6 V, the newly designed circuit produces an output dynamic range of 102 dB and the output voltage noise of 5.69 nV/√Hz, achieving a sensitivity of 12.53 V/pF.
A Review of Theoretical Knowledge and Practical Applications of Iron-Based Adsorbents for Removing Arsenic from Water
Authors: Nguyen Thi Hai , Nguyen Tien Vinh , Vigneswaran Saravanamuthu , Ha Nguyen Thi Hoang , Ratnaweera Harsha
Published: May 2023

Groundwater contamination by arsenic (As) is a serious issue in many countries, particularly in middle- and low-income nations. High concentrations of As in drinking water and groundwater cause serious health problems. Numerous technologies, such as ion exchange, oxidation, coagulation–flocculation, phytoremediation, membrane filtration, and adsorption, have been applied to remove As from the water environment. Among these technologies, adsorption is the preferred method in low- and middle-income countries due to its affordability, high efficiency, and ease of design, operation, and maintenance. Along with this technology, a variety of materials have been developed and investigated for their ability to remove As from water environments, though iron-based adsorbents have been reported to be the most effective. Thus, the aim of this paper is to briefly review the sources of As and the prevalence of As species in water bodies, as well as the currently available technologies for As removal. A detailed analysis of recent studies on iron-based adsorbents used to remove As from aqueous solutions in both laboratory conditions and field conditions is presented.
Using Large-Size Three-Dimensional Marine Electromagnetic Data for the Efficient Combined Investigation of Natural Hydrogen and Hydrocarbon Gas Reservoirs: A Geologically Consistent and Process-Oriented Approach with Implications for Carbon Footprint Reduction
Authors: Meju Max A. , Saleh Ahmad Shahir
Published: May 2023

The recycling or burial of carbon dioxide in depleted petroleum reservoirs and re-imagining exploration strategies that focus on hydrogen reservoirs (with any associated hydrocarbon gas as the upside potential) are a necessity in today’s environmental and geopolitical climate. Given that geologic hydrogen and hydrocarbon gases may occur in the same or different reservoirs, there will be gains in efficiency when searching for both resources together since they share some commonalities, but there is no geophysical workflow available yet for this purpose. Three-dimensional (3D) marine controlled-source electromagnetic (CSEM) and magnetotelluric (MT) methods provide valuable information on rock-and-fluid variations in the subsurface and can be used to investigate hydrogen and hydrocarbon reservoirs, source rocks, and the migration pathways of contrasting resistivity relative to the host rock. In this paper, a process-oriented CSEM-MT workflow is proposed for the efficient combined investigation of reservoir hydrocarbon and hydrogen within a play-based exploration and production framework that emphasizes carbon footprint reduction. It has the following challenging elements: finding the right basin (and block), selecting the right prospect, drilling the right well, and exploiting the opportunities for sustainability and CO2 recycling or burial in the appropriate reservoirs. Recent methodological developments that integrate 3D CSEM-MT imaging into the appropriate structural constraints to derive the geologically robust models necessary for resolving these challenges and their extension to reservoir monitoring are described. Instructive case studies are revisited, showing how 3D CSEM-MT models facilitate the interpretation of resistivity information in terms of the key elements of geological prospect evaluation (presence of source rocks, migration and charge, reservoir rock, and trap and seal) and understanding how deep geological processes control the distribution and charging of potential hydrocarbon, geothermal, and hydrogen reservoirs. In particular, evidence is provided that deep crustal resistivity imaging can map serpentinized ultramafic rocks (possible source rocks for hydrogen) in offshore northwest Borneo and can be combined with seismic reflection data to map vertical fluid migration pathways and their barrier (or seal), as exemplified by the subhorizontal detachment zones in Eocene shale in the Mexican Ridges fold belt of the southwest of the Gulf of Mexico, raising the possibility of using integrated geophysical methods to map hydrogen kitchens in different terrains. The methodological advancements and new combined investigative workflow provide a way for improved resource mapping and monitoring and, hence, a technology that could play a critical role in helping the world reach net-zero emissions by 2050.
Investigation on the Mineral Catalytic Graphitization of Anthracite during Series High Temperature Treatment
Authors: Cao Haiyue , Li Kuo , Zhang Hao , Liu Qinfu
Published: May 2023

Graphite can be artificially converted from anthracites under high temperatures; however, the exact mechanism through which inorganic minerals contribute to the graphitization process is still unknown. In light of this, several selected minerals in different amounts were added to demineralized anthracite coal. The anthracite–mineral mixtures were subjected to artificial graphitization experiments under temperatures ranging from 1700 to 2900 °C in the laboratory. The obtained series of coal-based graphites with various levels of graphitization were characterized by X-ray diffraction (XRD), and the derived structural parameters, such as d002 and FWHM (002), La, and Lc were used to compare the carbon structural evolution during the high temperature treatment and mineral catalytic graphitization. Moreover, the amorphous carbon of anthracite is eventually transformed into the highly ordered crystalline carbon of coal-based graphite. The five added minerals show interesting structural variation during the graphitization process, in which pyrite is decomposed into iron (Fe), illite, quartz, and kaolinite, which can react with disordered carbon in organic matter to form moissanite (SiC), while dolomite seems to react with sulfur to form oldhamite (CaS). At temperatures less than 2300 °C, the minerals could significantly enhance the catalytic effect. There is a clear difference in the catalytic effect of different minerals on graphitization. Kaolinite exhibits the strongest catalytic effect. The minerals dolomite, illite, and quartz only show a certain degree of catalysis. Pyrite, however, only has a limited effect on improving the degree of graphitization at a temperature of 1700 °C. However, once the temperature exceeds 2300 °C, the dominant factor controlling the graphitization of anthracite appears to be the temperature. According to the growth pattern at microcrystalline sizes (La and Lc), the minerals’ catalytic effects can be classified into three groups. The first group includes minerals that preferentially promote La growth, such as pyrite, illite, and quartz. The second group includes minerals that preferentially promote Lc growth, such as dolomite. Finally, kaolinite is in a separate group that promotes microcrystal growth in both the lateral and vertical directions simultaneously. The mechanisms of the minerals’ catalytic graphitization are discussed in this paper. The promotion role of minerals in the artificial graphitization process may help to optimize the graphitization process and reduce the process cost in the future.
Multi-Responsive Molecular Encapsulation and Release Based on Hydrogen-Bonded Azo-Macrocycle
Authors: Wu Jinyang , Sun Xuan , Li Xianghui , Li Xiaowei , Feng Wen , Yuan Lihua
Published: May 2023

Research on stimuli-responsive host–guest systems is at the cutting edge of supramolecular chemistry, owing to their numerous potential applications such as catalysis, molecular machines, and drug delivery. Herein, we present a multi-responsive host–guest system comprising azo-macrocycle 1 and 4,4′-bipyridinium salt G1 for pH-, photo-, and cation- responsiveness. Previously, we reported a novel hydrogen-bonded azo-macrocycle 1. The size of this host can be controlled through light-induced E↔Z photo-isomerization of the constituent azo-benzenes. The host is found in this work to be capable of forming stable complexes with bipyridinium/pyridinium salts, and implementing guest capture and release with G1 under light in a controlled manner. The binding and release of the guest in the complexes can also be easily controlled reversibly by using acid and base. Moreover, the cation competition-induced dissociation of the complex 1a2⊃G1 is achieved. These findings are expected to be useful in regulating encapsulation for sophisticated supramolecular systems.
XDH and XO Research and Drug Discovery—Personal History
Authors: Nishino Takeshi
Published: May 2023

The author will outline the research history of the main issues addressed in this paper. The author has worked on this research himself. XDH, which is responsible for purine degradation, is present in various organisms. However, conversion to XO only occurs in mammals. The molecular mechanism of this conversion was elucidated in this study. The physiological and pathological significance of this conversion is presented. Finally, enzyme inhibitors were successfully developed, two of which are used as therapeutic agents for gout. Their wide application potential is also discussed.
Separation of Flavonoids and Purification of Chlorogenic Acid from Bamboo Leaves Extraction Residues by Combination of Macroporous Resin and High-Speed Counter-Current Chromatography
Authors: Zhou Yifeng , Chen Meixu , Huo Xinyi , Xu Qilin , Wu Linlin , Wang Liling
Published: May 2023

Flavonoids are major active small-molecule compounds in bamboo leaves, which can be easily obtained from the bamboo leaves extraction residues (BLER) after the polysaccharides extraction. Six macroporous resins with different properties were screened to prepare and enrich isoorientin (IOR), orientin (OR), vitexin (VI), and isovitexin (IVI) from BLER, and the XAD-7HP resin with the best adsorption and desorption performance was selected for further evaluation. Based on the static adsorption experiments, the experimental results showed that the adsorption isotherm fitted well with the Langmuir isotherm model, and the adsorption process was better explained by the pseudo-second-order kinetic model. After the dynamic trial of resin column chromatography, 20 bed volume (BV) of upload sample and 60% ethanol as eluting solvent was used in a lab scale-up separation, and the results demonstrated that the content of four flavonoids could be increased by 4.5-fold, with recoveries between 72.86 and 88.21%. In addition, chlorogenic acid (CA) with purity of 95.1% was obtained in water-eluted parts during dynamic resin separation and further purified by high-speed countercurrent chromatography (HSCCC). In conclusion, this rapid and efficient method can provide a reference to utilize BLER to produce high-value-added food and pharmaceutical products.
Pharmacological Properties of Four Plant Species of the Genus Anabasis, Amaranthaceae
Authors: Shegebayev Zhanybek , Turgumbayeva Aknur , Datkhayev Ubaidilla , Zhakipbekov Kairat , Kalykova Assem , Kartbayeva Elmira , Beyatli Ahmet , Tastambek Kuanysh , Altynbayeva Gulmira , Dilbarkhanov Bassymbek , Akhelova Aiman , Anarbayeva Rabiga , Orynbassarova Kulpan
Published: May 2023

The genus Anabasis is a member of the family Amaranthaceae (former name: Chenopodiaceae) and includes approximately 102 genera and 1400 species. The genus Anabasis is one of the most significant families in salt marshes, semi-deserts, and other harsh environments. They are also renowned for their abundance in bioactive compounds, including sesquiterpenes, diterpenes, triterpenes, saponins, phenolic acids, flavonoids, and betalain pigments. Since ancient times, these plants have been used to treat various diseases of the gastrointestinal tract, diabetes, hypertension, and cardiovascular diseases and are used as an antirheumatic and diuretic. At the same time, the genus Anabasis is very rich in biologically active secondary metabolites that exhibit great pharmacological properties such as antioxidant, antibacterial, antiangiogenic, antiulcer, hypoglycemic, hepatoprotective, antidiabetic, etc. All of the listed pharmacological activities have been studied in practice by scientists from different countries and are presented in this review article to familiarize the entire scientific community with the results of these studies, as well as to explore the possibilities of using four plant species of the genus Anabasis as medicinal raw materials and developing medicines based on them.
A Simple and Effective Phosphine-Doping Technique for Solution-Processed Nanocrystal Solar Cells
Authors: Min Chenbo , Chen Yihui , Yang Yonglin , Wu Hongzhao , Guo Bailin , Wu Sirui , Huang Qichuan , Qin Donghuan , Hou Lintao
Published: May 2023

Solution-processed cadmium telluride (CdTe) nanocrystal (NC) solar cells offer the advantages of low cost, low consumption of materials and large-scale production via a roll-to-roll manufacture process. Undecorated CdTe NC solar cells, however, tend to show inferior performance due to the abundant crystal boundaries within the active CdTe NC layer. The introduction of hole transport layer (HTL) is effective for promoting the performance of CdTe NC solar cells. Although high-performance CdTe NC solar cells have been realized by adopting organic HTLs, the contact resistance between active layer and the electrode is still a large problem due to the parasitic resistance of HTLs. Here, we developed a simple phosphine-doping technique via a solution process under ambient conditions using triphenylphosphine (TPP) as a phosphine source. This doping technique effectively promoted the power conversion efficiency (PCE) of devices to 5.41% and enabled the device to have extraordinary stability, showing a superior performance compared with the control device. Characterizations suggested that the introduction of the phosphine dopant led to higher carrier concentration, hole mobility and a longer lifetime of the carriers. Our work presents a new and simple phosphine-doping strategy for further improving the performance of CdTe NC solar cells.
Physico-Mechanical Optimization and Antimicrobial Properties of the Bionanocomposite Films Containing Gallic Acid and Zinc Oxide Nanoparticles
Authors: Karami Azin , Ghanbarzadeh Babak , Fakhri Leila Abolghasemi , Falcone Pasquale M. , Hosseini Mohammadyar
Published: May 2023

The mechanical and physical properties of the bionanocomposite films based on κ-carrageenan (KC)–gelatin (Ge) containing zinc oxide nanoparticles (ZnONPs) and gallic acid (GA) were optimized using the response surface method, and the optimum amounts of 11.19 wt% GA and 1.20 wt% ZnONPs were obtained. The results of XRD, SEM, and FT-IR tests showed the uniform distribution of the ZnONPs and GA in the film microstructure, and suitable interactions between biopolymers and these additives, which led to increasing the structural cohesion of the biopolymer matrix and improving the physical and mechanical properties of the KC–Ge-based bionanocomposite. In the films containing gallic acid and ZnONPs, an antimicrobial effect was not observed against E. coli; however, the GA-loaded and optimum films show an antimicrobial effect against S. aureus. The optimum film showed a higher inhibition effect against S. aureus compared to the ampicillin- and gentamicin-loaded discs.
Systematically Study the Tensile and Compressive Behaviors of Diamond-like Carbon
Authors: Xu Jingxiang , Geng Yina , Chu Zhenhua , Hu Qingsong , Lei Yanhua , Wang Yang
Published: May 2023

It is important to understand the mechanical properties of diamond-like carbon (DLC) for use not only in frictionand wear-resistant coatings, but also in vibration reduction and damping increase at the layer interfaces. However, the mechanical properties of DLC are influenced by the working temperature and its density, and the applications of DLC as coatings are limited. In this work, we systematically studied the deformation behaviors of DLC under different temperatures and densities using compression and tensile testing of DLC by molecular dynamics (MD) methods. In our simulation results, the values of tensile stress and compressive stress decreased and tensile strain and compressive strain increased as the temperature increased from 300 K to 900 K during both tensile and compressive processes, indicating that the tensile stress and tensile strain depend on the temperature. During the tensile simulation, Young’s modulus of DLC models with different densities had a different sensitivity to the increase in temperature, and the DLC model with a high density was more sensitive than that with a low density, which was not seen in the compression process. We conclude that the Csp3-Csp2 transition leads to tensile deformation, while the Csp2-Csp3 transition and relative slip dominate compressive deformation.
Scientific Rationale for the Treatment of Cognitive Deficits from Long COVID
Authors: Fesharaki Zadeh Arman , Arnsten Amy F. T. , Wang Min
Published: May 2023

Sustained cognitive deficits are a common and debilitating feature of “long COVID”, but currently there are no FDA-approved treatments. The cognitive functions of the dorsolateral prefrontal cortex (dlPFC) are the most consistently afflicted by long COVID, including deficits in working memory, motivation, and executive functioning. COVID-19 infection greatly increases kynurenic acid (KYNA) and glutamate carboxypeptidase II (GCPII) in brain, both of which can be particularly deleterious to PFC function. KYNA blocks both NMDA and nicotinic-alpha-7 receptors, the two receptors required for dlPFC neurotransmission, and GCPII reduces mGluR3 regulation of cAMP-calcium-potassium channel signaling, which weakens dlPFC network connectivity and reduces dlPFC neuronal firing. Two agents approved for other indications may be helpful in restoring dlPFC physiology: the antioxidant N-acetyl cysteine inhibits the production of KYNA, and the α2A-adrenoceptor agonist guanfacine regulates cAMP-calcium-potassium channel signaling in dlPFC and is also anti-inflammatory. Thus, these agents may be helpful in treating the cognitive symptoms of long COVID.
Liver-Specific Bmal1 Depletion Reverses the Beneficial Effects of Nobiletin on Liver Cholesterol Homeostasis in Mice Fed with High-Fat Diet
Authors: Lu Zhitian , Li Xudong , Wang Min , Zhang Xiaojun , Zhuang Runxuan , Wu Fan , Li Wenxue , Zhu Wei , Zhang Bo
Published: May 2023

Nobiletin (NOB), a naturally occurring small-molecule compound abundant in citrus peels, has displayed potential lipid-lowering and circadian-enhancing properties in preclinical studies. However, the requirement of specific clock genes for the beneficial effects of NOB is not well understood. In the current study, mice with a liver-specific deletion of the core clock component, Bmal1—Bmal1LKO—were fed a high-fat diet (HFD) ad libitum for eight weeks, while NOB (200 mg/kg) was administered by daily oral gavage from the fifth week and throughout the last four weeks. NOB decreased liver triglyceride (TG) alongside the decreasing mRNA levels of de novo lipogenesis (DNL) genes in both Bmal1flox/flox and Bmal1LKO mice. NOB increased serum very low-density lipoprotein (VLDL) levels in Bmal1LKO mice, which was consistent with higher liver Shp and lower Mttp mRNA expression levels, the key genes that facilitate VLDL assembly and secretion. NOB decreased liver and serum cholesterol levels in the Bmal1flox/flox mice, consistent with lower Hmgcr and higher Cyp7a1, Cyp8b1, Gata4 and Abcg5 mRNA levels in the liver. In contrast, in the Bmal1LKO mice, NOB increased Hmgcr mRNA levels and had no effect on the above-mentioned genes related to bile acid synthesis and cholesterol excretion, which might contribute to the elevation of liver and serum cholesterol levels in NOB-treated Bmal1LKO mice. NOB inhibited hepatic DNL and decreased liver TG levels in HFD-fed mice independently of liver Bmal1, whereas liver-specific Bmal1 depletion reversed the beneficial effects of NOB on liver cholesterol homeostasis. The complex interactions between NOB, the circadian clock and lipid metabolism in the liver warrant further research.
Plasma Retinol Concentrations and Dietary Intakes of Mother–Infant Sets in Singleton versus Twin Pregnancy
Authors: Akbar Anum , Duvall Sarah , VanOrmer Matthew , Slotkowski Rebecca , Hahka Taija , Genaro-Mattos Thiago , Korade Zeljka , Hanson Corrine , Anderson Berry Ann , Thoene Melissa
Published: May 2023

Vitamin A (retinol) is essential for normal fetal development, but the recommendation for maternal dietary intake (Retinol Activity Equivalent, RAE) does not differ for singleton vs. twin pregnancy, despite the limited evaluation of retinol status. Therefore, this study aimed to evaluate plasma retinol concentrations and deficiency status in mother–infant sets from singleton vs. twin pregnancies as well as maternal RAE intake. A total of 21 mother–infant sets were included (14 singleton, 7 twin). The HPLC and LC-MS/HS evaluated the plasma retinol concentration, and data were analyzed using the Mann–Whitney U test. Plasma retinol was significantly lower in twin vs. singleton pregnancies in both maternal (192.2 vs. 312.1 vs. mcg/L, p = 0.002) and umbilical cord (UC) samples (102.5 vs. 154.4 vs. mcg/L, p = 0.002). The prevalence of serum-defined vitamin A deficiency (VAD) <200.6 mcg/L was higher in twins vs. singletons for both maternal (57% vs. 7%, p = 0.031) and UC samples (100% vs. 0%, p < 0.001), despite a similar RAE intake (2178 vs. 1862 mcg/day, p = 0.603). Twin pregnancies demonstrated a higher likelihood of vitamin A deficiency in mothers, with an odds ratio of 17.3 (95% CI: 1.4 to 216.6). This study suggests twin pregnancy may be associated with VAD deficiency. Further research is needed to determine optimal maternal dietary recommendations during twin gestation.
The Role of Nutrition in Obesity
Authors: Jiao Jingjing
Published: May 2023

This Special Issue of Nutrients, “Nutrients, Foods, Dietary Patterns and Obesity”, includes nine original articles that explore the role of eating behaviors, diet quality and dietary interventions in obesity and its potential mechanisms [...]
The In Vitro Effects of Romina Strawberry Extract on 3D Uterine Leiomyosarcoma Cells
Authors: Greco Stefania , Pellegrino Pamela , Giampieri Francesca , Capocasa Franco , Delli Carpini Giovanni , Battino Maurizio , Mezzetti Bruno , Giannubilo Stefano Raffaele , Ciavattini Andrea , Ciarmela Pasquapina
Published: May 2023

Leiomyosarcoma is an aggressive soft tissue sarcoma derived from the smooth muscle cells of the uterus. We tested the effect of Romina strawberry extract treatment on three-dimensional cultured uterine leiomyosarcoma cells. We established 3D cultures in agarose gel, where the cells seeded were able to form spheroids. We performed the observation and counting of the spheroids with a phase-contrast optical microscope, finding a decrease in the number of spheroids formed in the plates after 24 and 48 h treatment with 250 µg/mL of cultivar Romina strawberry extract. We also characterized the spheroids morphology by DNA binding fluorescent-stain observation, hematoxylin and eosin stain, and Masson’s trichrome stain. Finally, the real-time PCR showed a reduced expression of extracellular matrix genes after strawberry treatment. Overall, our data suggest that the fruit extract of this strawberry cultivar may be a useful therapeutic adjuvant for the management of uterine leiomyosarcoma.
Genetic Preference for Sweet Taste in Mothers Associates with Mother-Child Preference and Intake
Authors: Lif Holgerson Pernilla , Hasslöf Pamela , Esberg Anders , Haworth Simon , Domellöf Magnus , West Christina E. , Johansson Ingegerd
Published: May 2023

Taste perception is a well-documented driving force in food selection, with variations in, e.g., taste receptor encoding and glucose transporter genes conferring differences in taste sensitivity and food intake. We explored the impact of maternal innate driving forces on sweet taste preference and intake and assessed whether their children differed in their intake of sweet foods or traits related to sweet intake. A total of 133 single nucleotide polymorphisms (SNPs) in genes reported to associate with eating preferences were sequenced from saliva-DNA from 187 mother-and-child pairs. Preference and intake of sweet-, bitter-, sour-, and umami-tasting foods were estimated from questionnaires. A total of 32 SNP variants associated with a preference for sweet taste or intake at a p-value < 0.05 in additive, dominant major, or dominant minor allele models, with two passing corrections for multiple testing (q < 0.05). These were rs7513755 in the TAS1R2 gene and rs34162196 in the OR10G3 gene. Having the T allele of rs34162196 was associated with higher sweet intake in mothers and their children, along with a higher BMI in mothers. Having the G allele of rs7513755 was associated with a higher preference for sweets in the mothers. The rs34162196 might be a candidate for a genetic score for sweet intake to complement self-reported intakes.
Preparation of Red Ginseng Marc-Derived Gintonin and Its Application as a Skin Nutrient
Authors: Lee Rami , Kim Ji-Hun , Hwang Hongik , Rhim Hyewhon , Hwang Sung-Hee , Cho Ik-Hyun , Kim Do-Geun , Kim Hyoung-Chun , Nah Seung-Yeol
Published: May 2023

Ginseng is one of the traditional herbal medicines for tonic. Gintonin is a new material derived from white/red ginseng and its lysophosphatidic acids (LPAs) play as a ligand for G protein-coupled LPA receptors. Korean red ginseng marc (KRGM) is a by-product after the KRG processes. We developed a low-cost/high-efficiency method for KRGM gintonin production. We further studied the KRGM gintonin-mediated anti-skin aging effects under UVB exposure using human dermal fibroblasts (HDFs). KRGM gintonin yield is about 8%. KRGM gintonin contains a high amount of LPA C18:2, lysophosphatidylcholine (LPC), and phosphatidylcholine (PC), which is similar to white ginseng gintonin. KRGM gintonin induced [Ca2+]i transient via LPA1/3 receptors and increased cell viability/proliferation under UVB exposure. The underlying mechanisms of these results are associated with the antioxidant action of KRGM gintonin. KRGM gintonin attenuated UVB-induced cell senescence by inhibiting cellular β-galactosidase overexpression and facilitated wound healing. These results indicate that KRGM can be a novel bioresource of KRGM gintonin, which can be industrially utilized as new material for skin nutrition and/or skin healthcare.
Translation of the Satter’s Division of Responsibility in Feeding Questionnaire into Brazilian Portuguese: A Cross-Sectional Study
Authors: Dusi Rafaella , Botelho Raquel Braz Assunção , Nakano Eduardo Yoshio , Queiroz Fabiana Lopes Nalon de , Zandonadi Renata Puppin
Published: May 2023

This cross-sectional study aimed to translate and perform a psychometric analysis (evaluation of reproducibility and internal consistency) of the sDOR.2-6y™ into Brazilian Portuguese. The translation and back-translation followed the protocol required by the NEEDs Center, and the approved version was called “sDOR.2-6y™—Português-Brasil”. The approved version was submitted to a test–retest round to verify its reproducibility through the Intraclass Correlation Coefficient (ICC). A pilot study was performed to assess the internal consistency of the instrument. The reproducibility analysis (n = 23) showed a total ICC of 0.945. With the data from the pilot study (n = 384), the internal consistency evaluation was analyzed through Cronbach’s alpha coefficient, and the instrument obtained an overall Cronbach’s alpha of 0.301. The translation of the sDOR.2-6y™ into Brazilian Portuguese is the first and only tool available for the Brazilian population to exclusively assess the division of responsibility in feeding, which is essential to the academic community, health professionals, and research on child feeding. Therefore, this instrument in Brazilian Portuguese will allow future research on the division of responsibility in feeding among those responsible for children in Brazil.
Characterization of Collagen Binding Activity of Clostridium perfringens Strains Isolated from Broiler Chickens
Authors: Sun Zhifeng , Lu Mingmin , Lillehoj Hyun , Lee Youngsub , Goo Doyun , Yuan Baohong , Yan Xianghe , Li Charles
Published: May 2023

Clostridium perfringens is the etiological agent for necrotic enteritis (NE) in broiler chickens, which causes a substantial economic loss of an estimated USD 6 billion annually in the global poultry industry. Collagen adhesion is involved in the NE pathogenesis in poultry. In this study, the binding capabilities of chicken C. perfringens isolates of various genetic backgrounds (netB−tpeL−, netB+tpeL−, netB+tpeL+) to collagen types I–V and gelatin were examined, and the putative adhesin protein cnaA gene was investigated at the genomic level. In total, 28 C. perfringens strains from healthy and NE-inflicted sick chickens were examined. The results on collagen adhesin-encoding gene cnaA by the quantitative-PCR results indicated that netB−tpeL− isolates had much lower copies of the detectable cnaA gene than netB+ isolates (10 netB+tpeL− isolates, 5 netB+tpeL+ isolates). Most of the virulent C. perfringens isolates demonstrated collagen-binding abilities to types I–II and IV–V, while some strains showed weak or no binding to collagen type III and gelatin. However, the netB+tpeL+ isolates showed significantly higher binding capabilities to collagen III than netB−tpeL− and netB+tpeL− isolates. The data in this study suggest that the collagen-binding capability of clinical C. perfringens isolates correlates well with their NE pathogenicity levels, especially for C. perfringens isolates carrying genes encoding crucial virulence factors and virulence-associated factors such as netB, cnaA, and tpeL. These results indicate that the presence of the cnaA gene may be correlated with C. perfringens virulence (particularly for netB+ isolates).
Vertebrate and Invertebrate Animal and New In Vitro Models for Studying Neisseria Biology
Authors: Girgis Michael , Christodoulides Myron
Published: May 2023

The history of Neisseria research has involved the use of a wide variety of vertebrate and invertebrate animal models, from insects to humans. In this review, we itemise these models and describe how they have made significant contributions to understanding the pathophysiology of Neisseria infections and to the development and testing of vaccines and antimicrobials. We also look ahead, briefly, to their potential replacement by complex in vitro cellular models.
Biological Control of Phytopathogens: Mechanisms and Applications
Authors: Hartmann Anton , Proença Diogo Neves
Published: May 2023

According to the inherent ecological mechanisms within community structures, organismic interactions are mediated by chemical structures and signaling molecules as well as enzymatic activities targeting the vital activities of microbial competitors [...]
Spasmolytic and Uroprotective Effects of Apigenin by Downregulation of TGF-β and iNOS Pathways and Upregulation of Antioxidant Mechanisms: In Vitro and In Silico Analysis
Authors: Saima Saima , Anjum Irfan , Mobashar Aisha , Jahan Shah , Najm Saima , Nafidi Hiba-Allah , Bin Jardan Yousef A. , Bourhia Mohammed
Published: May 2023

Apigenin is a phytochemical obtained from Chamomilla recutita. Its role in interstitial cystitis is not yet known. The present study is aimed at understanding the uroprotective and spasmolytic effects of apigenin in cyclophosphamide-induced interstitial cystitis. The uroprotective role of apigenin was analyzed by qRT-PCR, macroscopic analysis, Evans blue dye leakage, histological evaluation, and molecular docking. The spasmolytic response was measured by adding cumulative concentrations of apigenin to isolated bladder tissue pre-contracted with KCl (80 mM) and carbachol (10−9–10−4) on non-incubated and pre-incubated tissues with atropine, 4DAMP, methoctramine, glibenclamide, barium chloride, nifedipine, indomethacin, and propranolol. Apigenin inhibited pro-inflammatory cytokines (IL-6, TNF-α and TGF 1-β) and oxidant enzymes (iNOS) while increasing antioxidant enzymes (SOD, CAT, and GSH) in CYP-treated groups compared to the control. Apigenin restored normal tissue of the bladder by decreasing pain, edema, and hemorrhage. Molecular docking further confirmed the antioxidant and anti-inflammatory properties of apigenin. Apigenin produced relaxation against carbachol-mediated contractions, probably via blockade of M3 receptors, KATP channels, L-type calcium channels, and prostaglandin inhibition. While the blockade of M2 receptors, KIR channels, and β-adrenergic receptors did not contribute to an apigenin-induced spasmolytic effect, apigenin presented as a possible spasmolytic and uroprotective agent with anti-inflammatory, antioxidant effects by attenuating TGF-β/iNOS-related tissue damage and bladder muscle overactivity. Thus, it is a potential agent likely to be used in treatment of interstitial cystitis.
Transient Coatings from Nanoparticles Achieving Broad-Spectrum and High Antimicrobial Performance
Authors: Zaia Rachel , Quinto Giovanna M. , Camargo Livia C. S. , Ribeiro Rodrigo T. , Carmona-Ribeiro Ana M.
Published: May 2023

Cationic and hydrophilic coatings based on casting and drying water dispersions of two different nanoparticles (NPs) onto glass are here described and evaluated for antimicrobial activity. Discoid cationic bilayer fragments (BF) surrounded by carboxy-methylcellulose (CMC) and poly (diallyl dimethyl ammonium) chloride (PDDA) NPs and spherical gramicidin D (Gr) NPs dispersed in water solution were cast onto glass coverslips and dried, forming a coating quantitatively evaluated against Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. From plating and colony forming units (CFU) counting, all strains interacting for 1 h with the coatings lost viability from 105 to 106, to zero CFU, at two sets of Gr and PDDA doses: 4.6 and 25 μg, respectively, or, 0.94 and 5 μg, respectively. Combinations produced broad spectrum, antimicrobial coatings; PDDA electrostatically attached to the microbes damaging cell walls, allowing Gr NPs interaction with the cell membrane. This concerted action promoted optimal activity at low Gr and PDDA doses. Further washing and drying of the deposited dried coatings showed that they were washed out so that antimicrobial activity was no longer present on the glass surface. Significant applications in biomedical materials can be foreseen for these transient coatings.
Live Cell Imaging by Förster Resonance Energy Transfer Fluorescence to Study Trafficking of PLGA Nanoparticles and the Release of a Loaded Peptide in Dendritic Cells
Authors: Liu Mengshan , Lau Chun Yin Jerry , Cabello Irene Trillo , Garssen Johan , Willemsen Linette E. M. , Hennink Wim E. , van Nostrum Cornelus F.
Published: May 2023

Our previous study demonstrated that a selected β-lactoglobulin-derived peptide (BLG-Pep) loaded in poly(lactic-co-glycolic acid) (PLGA) nanoparticles protected mice against cow’s milk allergy development. However, the mechanism(s) responsible for the interaction of the peptide-loaded PLGA nanoparticles with dendritic cells (DCs) and their intracellular fate was/were elusive. Förster resonance energy transfer (FRET), a distance-dependent non-radioactive energy transfer process mediated from a donor to an acceptor fluorochrome, was used to investigate these processes. The ratio of the donor (Cyanine-3)-conjugated peptide and acceptor (Cyanine-5) labeled PLGA nanocarrier was fine-tuned for optimal (87%) FRET efficiency. The colloidal stability and FRET emission of prepared NPs were maintained upon 144 h incubation in PBS buffer and 6 h incubation in biorelevant simulated gastric fluid at 37 °C. A total of 73% of Pep-Cy3 NP was internalized by DCs as quantified using flow cytometry and confirmed using confocal fluorescence microscopy. By real-time monitoring of the change in the FRET signal of the internalized peptide-loaded nanoparticles, we observed prolonged retention (for 96 h) of the nanoparticles-encapsulated peptide as compared to 24 h retention of the free peptide in the DCs. The prolonged retention and intracellular antigen release of the BLG-Pep loaded in PLGA nanoparticles in murine DCs might facilitate antigen-specific tolerance induction.
RETRACTED: Zhou et al. Pyrvinium Treatment Confers Hepatic Metabolic Benefits via β-Catenin Downregulation and AMPK Activation. Pharmaceutics 2021, 13, 330
Authors: Zhou Shiwei , Obianom Obinna N. , Huang Jiangsheng , Guo Dong , Yang Hong , Li Qing , Shu Yan
Published: May 2023

The authors and the journal retract the article, ‘Pyrvinium Treatment Confers Hepatic Metabolic Benefits via β-Catenin Downregulation and AMPK Activation’ [...]
Combined Biological and Numerical Modeling Approach for Better Understanding of the Cancer Viability and Apoptosis
Authors: Živanović Marko , Gazdić Janković Marina Gazdić , Ramović Hamzagić Amra , Virijević Katarina , Milivojević Nevena , Pecić Katarina , Šeklić Dragana , Jovanović Milena , Kastratović Nikolina , Mirić Ana , Đukić Tijana , Petrović Ivica , Jurišić Vladimir , Ljujić Biljana , Filipović Nenad
Published: May 2023

Nowadays, biomedicine is a multidisciplinary science that requires a very broad approach to the study and analysis of various phenomena essential for a better understanding of human health. This study deals with the use of numerical simulations to better understand the processes of cancer viability and apoptosis in treatment with commercial chemotherapeutics. Starting from many experiments examining cell viability in real-time, determining the type of cell death and genetic factors that control these processes, a lot of numerical results were obtained. These in vitro test results were used to create a numerical model that gives us a new angle of observation of the proposed problem. Model systems of colon and breast cancer cell lines (HCT-116 and MDA-MB-231), as well as a healthy lung fibroblast cell line (MRC-5), were treated with commercial chemotherapeutics in this study. The results indicate a decrease in viability and the appearance of predominantly late apoptosis in the treatment, a strong correlation between parameters. A mathematical model was created and employed for a better understanding of investigated processes. Such an approach is capable of accurately simulating the behavior of cancer cells and reliably predicting the growth of these cells.
A Novel Control Method of Enterococcus faecalis by Co-Treatment with Protamine and Calcium Hydroxide
Authors: Abe Yu , Honda Michiyo
Published: May 2023

Enterococcus faecalis (E. faecalis), a gram-positive facultative anaerobic bacterium, is likely to survive root canal treatment because of its extremely high alkaline tolerance, which may contribute to the refractory nature of apical periodontitis (AP). In this study, protamine was combined with calcium hydroxide to evaluate its efficacy in killing E. faecalis. First, the antibacterial activity of protamine against E. faecalis was investigated. Protamine reduced the E. faecalis growth rate at concentrations above the MIC (250 μg/mL), but was not bactericidal at any of the concentrations tested. Next, we investigated the calcium hydroxide tolerance of E. faecalis, using a 10% 310 medium, adjusted for pH by adding a calcium hydroxide solution. The results showed that E. faecalis could survive and proliferate in alkaline environments up to pH 10. However, the complete killing of E. faecalis was observed when protamine (250 μg/mL) was added. In addition, compared with treatment with protamine and calcium hydroxide alone, membrane damage and internalization of protamine into the cytoplasm of E. faecalis were enhanced. Therefore, the synergistic increase in antibacterial activity may be related to the action of both antimicrobial agents on the cell membrane. In conclusion, co-treatment with protamine and calcium hydroxide seems to be very effective in sterilizing E. faecalis, and has the potential to provide a novel control method against E. faecalis for root canal treatment.
How Naive Is Contentful Moral Perception?
Authors: Werner Preston J.
Published: May 2023

According to contentful moral perception (CMP), moral properties can be perceived in the same sense as tables, tigers, and tomatoes. Recently, Heather Logue (2012) has distinguished between two potential ways of perceiving a property. A Kantian Property (KP) in perception is one in which a perceiver’s access involves a detection of the property via a representational vehicle. A Berkeleyan Property (BP) in perception is one in which a perceiver’s access to the property involves that property as partly constitutive of the experience itself. In this paper, I set aside generalized arguments in favor of one view or another, and instead ask whether proponents of CMP have reasons to understand moral perception as Kantian or Berkeleyan. I explore three possible explanatory differences—(a) explaining the intrinsic motivating force of moral perceptions, (b) providing a metasemantics for moral properties, and (c) providing an epistemology of the normative authority of moral properties.
Mining Salt Tolerance SNP Loci and Prediction of Candidate Genes in the Rice Bud Stage by Genome-Wide Association Analysis
Authors: Wang Rui , Zhou Zhenzhen , Xiong Mengyuan , Du Mingyu , Lin Xingxing , Liu Cuiping , Lu Mingwei , Liu Zhengbo , Chang Yinping , Liu Erbao
Published: May 2023

Mining salt tolerance genes is significant for breeding high-quality salt-tolerant rice varieties in order to improve the utilization of saline–alkaline land. In this study, 173 rice accessions were measured for their germination potential (GP), germination rate (GR), seedling length (SL), root length (RL), germination potential relative to salt damage rate (GPR), germination rate relative to salt damage rate (GRR), seedling length relative to salt damage rate (SLR), relative salt damage rate at the germination stage (RSD) and comprehensive relative salt damage rate in the early seedling stage (CRS) under normal and salt stress conditions. Genome-wide association analysis was performed with 1,322,884 high-quality SNPs obtained by resequencing. Eight quantitative trait loci (QTLs) related to salt tolerance traits at the germination stage were detected in 2020 and 2021. They were related to the GPR (qGPR2) and SLR (qSLR9), which were newly discovered in this study. Three genes were predicted as salt tolerance candidate genes: LOC_Os02g40664, LOC_Os02g40810, and LOC_Os09g28310. At present, marker-assisted selection (MAS) and gene-edited breeding are becoming more widespread. Our discovery of candidate genes provides a reference for research in this field. The elite alleles identified in this study may provide a molecular basis for cultivating salt-tolerant rice varieties.
Evidence That PbrSAUR72 Contributes to Iron Deficiency Tolerance in Pears by Facilitating Iron Absorption
Authors: Guo Guoling , Yu Tao , Zhang Haiyan , Chen Meng , Dong Weiyu , Zhang Shuqin , Tang Xiaomei , Liu Lun , Heng Wei , Zhu Liwu , Jia Bing
Published: May 2023

Iron is an essential trace element for plants; however, low bioactive Fe in soil continuously places plants in an Fe-deficient environment, triggering oxidative damage. To cope with this, plants make a series of alterations to increase Fe acquisition; however, this regulatory network needs further investigation. In this study, we found notably decreased indoleacetic acid (IAA) content in chlorotic pear (Pyrus bretschneideri Rehd.) leaves caused by Fe deficiency. Furthermore, IAA treatment slightly induced regreening by increasing chlorophyll synthesis and Fe2+ accumulation. At that point, we identified PbrSAUR72 as a key negative effector output of auxin signaling and established its close relationship to Fe deficiency. Furthermore, the transient PbrSAUR72 overexpression could form regreening spots with increased IAA and Fe2+ content in chlorotic pear leaves, whereas its transient silencing does the opposite in normal pear leaves. In addition, cytoplasm-localized PbrSAUR72 exhibits root expression preferences and displays high homology to AtSAUR40/72. This promotes salt tolerance in plants, indicating a putative role for PbrSAUR72 in abiotic stress responses. Indeed, transgenic plants of Solanum lycopersicum and Arabidopsis thaliana overexpressing PbrSAUR72 displayed less sensitivity to Fe deficiency, accompanied by substantially elevated expression of Fe-induced genes, such as FER/FIT, HA, and bHLH39/100. These result in higher ferric chelate reductase and root pH acidification activities, thereby hastening Fe absorption in transgenic plants under an Fe-deficient condition. Moreover, the ectopic overexpression of PbrSAUR72 inhibited reactive oxygen species production in response to Fe deficiency. These findings contribute to a new understanding of PbrSAURs and its involvement in Fe deficiency, providing new insights for the further study of the regulatory mechanisms underlying the Fe deficiency response.
Controlled Release and Cell Viability of Ketoconazole Incorporated in PEG 4000 Derivatives
Authors: Inácio Carolina R. , Nascimento Gabriel S. , Barboza Ana Paula M. , Neves Bernardo R. A. , Andrade Ângela Leão , Teixeira Gabriel M. , Sousa Lucas R. D. , de A. Vieira Paula M. , Novack Kátia M. , dos Santos Viviane M. R.
Published: May 2023

In recent years, polymeric materials have been gaining prominence in studies of controlled release systems to obtain improvements in drug administration. These systems present several advantages compared with conventional release systems, such as constant maintenance in the blood concentration of a given drug, greater bioavailability, reduction of adverse effects, and fewer dosages required, thus providing a higher patient compliance to treatment. Given the above, the present work aimed to synthesize polymeric matrices derived from polyethylene glycol (PEG) capable of promoting the controlled release of the drug ketoconazole in order to minimize its adverse effects. PEG 4000 is a widely used polymer due to its excellent properties such as hydrophilicity, biocompatibility, and non-toxic effects. In this work, PEG 4000 and derivatives were incorporated with ketoconazole. The morphology of polymeric films was observed by AFM and showed changes on the film organization after drug incorporation. In SEM, it was possible to notice spheres that formed in some incorporated polymers. The zeta potential of PEG 4000 and its derivatives was determined and suggested that the microparticle surfaces showed a low electrostatic charge. Regarding the controlled release, all the incorporated polymers obtained a controlled release profile at pH 7.3. The release kinetics of ketoconazole in the samples of PEG 4000 and its derivatives followed first order for PEG 4000 HYDR INCORP and Higuchi for the other samples. Cytotoxicity was determined and PEG 4000 and its derivatives were not cytotoxic.