bluuebunny/crossref_metadata_2025 huggingface dataset:
An online therapist-guided ultra-brief treatment for depression and anxiety: a randomized controlled trial
Authors: Bisby Madelyne A. , Balakumar Tanya , Scott Amelia J. , Titov Nickolai , Dear Blake F.
Published: September 2023

Background

There are many barriers to engaging in current psychological treatments, including time, cost, and availability. Ultra-brief treatments overcome some of these barriers by delivering therapeutic information and skills using significantly less time than standard-length treatments. We developed a therapist-guided online ultra-brief treatment for depression and anxiety and compared it to an existing 8-week, 5-lesson therapist-guided standard-length treatment and a waitlist control.

Methods

In a randomized controlled trial, adults with self-reported depression or anxiety were randomized (1:1:1) to the ultra-brief treatment, standard-length treatment, or waitlist control. The primary outcomes were depression symptoms and anxiety symptoms assessed at baseline, 5-weeks later, 9-weeks later (primary timepoint), and 3-months later. The trial was prospectively registered.

Results

Between 7 February 2022, and 16 August 2022, 242 participants were enrolled in the ultra-brief treatment (n = 85), standard-length treatment (n = 80), and waitlist control (n = 77). Participants were mostly women with an average age of 48.56 years. At 9-weeks post-baseline, participants in the ultra-brief treatment group reported significantly lower depression (between groups d = 0.41) and anxiety (d = 0.53) than the waitlist control. The ultra-brief treatment was non-inferior for anxiety at both 9-weeks and 3-months follow-up. Non-inferiority for depression was observed at 9-weeks.

Conclusions

The online ultra-brief treatment resulted in significant reductions in depression and anxiety that were non-inferior to a longer treatment course after 9-weeks. Remotely delivered ultra-brief treatments have the potential to provide accessible and effective care for those who cannot, or would prefer not to, access longer psychological interventions.
Women’s discursive agency in transitional justice policy-making: A feminist institutionalist approach
Authors: Kostovicova Denisa , Popovski Vesna
Published: August 2023

Scholars have studied how women’s domestic and transnational civil society activism addresses the gendered nature of transitional justice. In contrast, they have paid scant attention to women’s impact on transitional justice policy-making in institutions. We leverage the feminist institutionalist perspective that makes visible gendered norms, rules, and discourses in institutions. Homing in on women’s influence in parliaments where women are outnumbered by men and marginalised by adversarial discourse, we develop a conceptualisation of women’s discursive agency. Foregrounding discourse in women’s ability to drive change, women’s agency is enacted through their linguistic communication style and substantive normative positions that constitute micro- and macro-level structures of domination. Quantitative and qualitative discourse analysis is applied to a corpus of parliamentary questions about transitional justice in the Croatian parliament from 2004 to 2020. Our results show that women adopt the adversarial style of questioning, which they use to broaden the scope of entitlements and press for reparations for female and male victims. They overcome constraints posed by partisanship and ideology, while constraints of nationalism are less easily broken. The article advances feminist transitional justice by demonstrating how women’s language contributes to dismantling men’s policy domination in institutions, with implications for mixed-sex interactions in non-institutional domains.
Single and multiple humanoid path planning using Hill valley approach applied to gravitational drift in Gravitational search algorithm
Authors: Vikas  , Parhi Dayal R.
Published: August 2023

In this paper, the Hill Valley (HV) approach is applied to the drifting masses or agents in the basic Gravitational Search Algorithm (GSA) for the path planning of humanoid robots. The drift in lighter masses toward the heavier mass creates a localized area, where the probability of obtaining a globally optimal solution is very high. So, the present work is focused on exploiting the area to tackle local optima and provide the best steering angle for the humanoids to navigate. The HV approach is applied to the basic GSA model, at the later stages, to improve the overall computational time and cost. The robustness of the proposed controller was tested in both simulation and experimental environments and compared with the previous research. The results obtained from the proposed controller showed a significant improvement in the overall path length and time taken. Path smoothness was also given equal importance during path planning to ensure stability. The multi-robot navigational scheme was performed using the Dining Philosopher’s model to avoid dynamic collision among the humanoids. The percentage deviation in the results was within the acceptable limits. To further check the effectiveness of the proposed technique, the proposed approach was compared with the vision-based navigation in danger space.
Kisspeptin decreases the adverse effects of human ovarian vitrification by regulating ROS-related apoptotic occurrences
Authors: Tavakoli Anahita , Aliakbari Fereshteh , Soleimani Mehranjani Malek
Published: September 2023

Kisspeptin is characterized as a neuropeptide with a pivotal function in female and male infertility, and its antioxidant properties have been demonstrated. In this study, the effects of kisspeptin on the improvement of the vitrification and thawing results of human ovarian tissues were investigated. In this work, 12 ovaries from patients who underwent hysterectomy were collected laparoscopically, and then 32 samples from each of their tissues were taken. Haematoxylin and eosin (H&E) staining was performed to check the normality of the ovarian tissue and, subsequently, the samples were allocated randomly into four groups, including: (1) fresh (control), (2) vitrification, (3) vitrified + 1 μM kisspeptin, and (4) vitrified + 10 μM kisspeptin groups. After vitrification, thawing, and tissue culture processes, H&E staining for tissue quality assessment, terminal deoxynucleotidyl transferase dUTP nick end labelling assay for apoptosis evaluation, and malondialdehyde (MDA), superoxide dismutase (SOD), and ferric reducing ability of plasma tests for oxidative stress appraisal were carried out. Our histological results showed incoherency of ovarian tissue morphology in the vitrification group compared with other groups. Other findings implicated increased apoptosis rate and MDA concentration and reduced SOD activity and total antioxidant capacity (TAC) in the vitrification group compared with the control group (P < 0.05). Moreover, decreased apoptosis rate and MDA concentration, and increased TAC and SOD function were observed in the vitrification with kisspeptin groups (1 μM and 10 μM) compared with the vitrified group (P < 0.05). Our reports express that kisspeptin is an effective agent to overcome the negative effects of vitrification by regulating reactive oxygen species-related apoptotic processes.
An Introduction
Authors: Hilton Leon J. , Johnson Jasmine
Published: September 2023

Performances and performative instantiations respond to the urgencies of “pressure” and its effects. What is the work of performance when wrung by such pressures as financial constraint, racial subordination, misogyny, and sexism?
Claudia Bucher in Five Movements
Authors: Cheng Meiling
Published: September 2023

For close to four decades, Claudia Bucher’s “scientart” has created myriad experiential habitats for her performing body, while inviting others to join her imaginary leaps. From personifying an outer-space lichen colony to meditating midair as an “anemochore kite,” Bucher demonstrates empathic kinships with other beings and nonbeings, calling forth alternative eco-systems in which sentience thrives as the common denominator for all inhabitants.
Religiosity matters: assessing competing explanations of support for secularism in Quebec and Canada
Authors: Bibeau Alexis , Brie Evelyne , Dufresne Yannick , Gagné Gilles
Published: September 2023

Secularism—i.e., the separation between the state and religious institutions—is a fundamental characteristic of liberal democracies, yet support for secular arrangements varies significantly across Western countries. In Canada, such attitudinal divergences are observable at the regional level, with citizens from Quebec displaying higher levels of support for secularism than other Canadians. In this paper, we test three hypotheses to account for this regional discrepancy: religiosity, liberal values, and out-group prejudice. Using data from an online panel survey (n = 2,000), our findings suggest that support for secularism in Quebec is mostly explained by the province's lower baseline levels of religiosity, anticlerical feelings, and by its distinctive understanding of liberalism. These factors are likely to result from Quebec's unique religious and sociohistorical history. Results also suggest that while negative feelings toward religious minorities are positively correlated with support for secularism across the entire country, negative feelings toward ethnic minorities are associated with lower support for secularism in Quebec. These findings disprove the commonly held assumption according to which support for secularism is driven by ethnic prejudice in Quebec.
High gain metasurface integrated millimeter-wave planar antenna
Authors: Tahir Muhammad Usman , Rafique Umair , Ahmed Muhammad Mansoor , Abbas Syed Muzahir , Iqbal Shahid , Wong Sai-Wai
Published: September 2023

A metasurface reflector-backed wideband planar antenna is designed for millimeter-wave (mmWave) applications. A simple meandering structure is used for radiation element design, while the back side consists of a partial ground plane and parasitic elements. The utilization of meander-shaped element led to small antenna dimensions. The partial ground plane is used to achieve wide bandwidth, while the parasitic elements are used to improve the impedance matching toward higher frequency bands. To achieve high gain and directional radiation characteristics, an array of metasurfaces is placed behind the radiating element. It is observed from the simulated results that the proposed antenna system offers 17.72 GHz of impedance bandwidth in the operating range of 22.28–40 GHz, while the measured impedance bandwidth is noted to be 15.8 GHz, ranging from 23 to 38.8 GHz. Furthermore, it is observed that a metasurface-based planar antenna tends to achieve a peak gain of ≈9 dBi in the band of interest.
Ayutthaya's Seventeenth-century Shi‘ite Muslim Enclave: A Reassessment
Authors: Joll Christopher M.
Published: September 2023

The primary purpose of this article is to reconstruct the date, location, and significance of Ayutthaya's Shi‘ite enclave within the former Siamese capital during the seventeenth century. This reassessment is based on a mixture of Persian, Thai, and European sources that clarify the confused picture generated by European cartographers that has for too long cast a shadow over Muslim studies in Thailand. Following a summary of extant explanations and a description of my primary sources and methodological approach, I summarise two aspects of Muslim presence in Ayutthaya. First, I introduce readers to connections between the incremental growth of the Muslim presence in Ayutthaya during the sixteenth century with geopolitical developments on the eastern littoral of the Bay of Bengal. Second, I present the range of accounts provided in primary sources specifically mentioning Ayutthaya's Muslim enclave. Having orientated readers to the origins of the Muslim presence within Ayutthaya's citadel, I incrementally introduce annotated portions of Thai and European maps. These clarify confusion about where and when this Shi‘ite mosque was constructed. I conclude with comments about how this reassessment brings into focus the presence of Shi‘ite ‘alid piety, Shi‘ite polemics about local Sunnis, Siamese conversion to Shi‘ism, and distinctions between these “Moors” and “Malays.”
Sprachwahrnehmung im Säuglingsalter
Authors: Altvater-Mackensen Nicole
Published: September 2023

Zusammenfassung: Der Artikel gibt einen Überblick über die Entwicklung der Sprachwahrnehmung im ersten Lebensjahr. Er zeichnet den Entwicklungsverlauf von den frühesten Hörerfahrungen bis zum ersten gesprochenen Wort nach und geht darauf ein: (i) wie vorgeburtliche Erfahrungen das Hören bei Neugeborenen prägen; (ii) wie die Sprachwahrnehmung sich im Säuglingsalter verändert und auf die Umgebungssprache einstellt; (iii) welche Faktoren die Veränderung in der Sprachwahrnehmung beeinflussen; (iv) wie theoretische Modelle diese Veränderungen erklären; und (v) welche Rolle die perzeptuelle Entwicklung im ersten Lebensjahr für die weitere Sprachentwicklung spielt.
The Cluster Virtual Observatory for ULF Waves
Authors: Constantinescu O. D. , Fornaçon K. H. , Motschmann U. , Glassmeier K. H. , Richter I. , Plaschke F.
Published: September 2023

Since its launch in 2000, the Cluster fleet visited a vast domain of the circum‐terrestrial environment, from the upstream solar wind and the distant tail, down to the plasmasphere, scanning in detail all magnetospheric regions during over two solar cycles. This led to an unprecedentedly rich data collection of multi‐point measurements which will be used for years to come to decipher the mechanisms of Solar‐Terrestrial interactions. The large volume of data gathered by Cluster requires special strategies to make efficient use of it. To address this issue we constructed a browsable database containing parameters of the detected Ultra low frequency waves and of the spacecraft formation geometry. The primary data used to derive the parameters are the magnetic field, the electric field and the electron density. The data is resampled to a cadence of 1 s and processed using a sliding analysis window of 2,048 s with a step of 256 s over 24 hr intervals. This results in time‐frequency arrays for each parameter covering the 0.5 mHz to 0.5 Hz frequency range. The database is accessible at http://plasma.spacescience.ro/cluster.html. In total there are 47 wave parameters in the database, among them being the ellipticity, the degree of polarization, the (unsigned) wave vector direction, and the Poynting vector. Plots for the planarity, elongation, and degeneration of the Cluster tetrahedron are also available. At the moment, the database covers measurements made between 01 January 2001 and 31 December 2020 with more data being added in time. Here we present this database, discuss the methods used to derive the parameters and give practical examples.
Enhanced Polar Vortex Predictability Following Sudden Stratospheric Warming Events
Authors: Rupp Philip , Spaeth Jonas , Garny Hella , Birner Thomas
Published: August 2023

Sudden stratospheric warming (SSW) events can form a window of forecast opportunity for polar vortex predictions on subseasonal‐to‐seasonal time scales. Analyzing numerical ensemble simulations, we quantify the associated enhanced predictability due to reduced upward planetary wave fluxes during the mostly radiatively driven recovery phase following SSWs. Ensembles that predict an SSW show reduced ensemble spread in terms of polar vortex strength for several weeks to follow, as well as a corresponding reduction in forecast errors. This increased predictability is particularly pronounced for strong SSWs and even occurs if not all ensemble members predict a major SSW. Furthermore, we found a direct impact of the occurrence of SSWs on the date of the final warming (FW): the decrease in upward wave fluxes delays the FW significantly. The reduced spread after SSWs and the delay in FW date have potentially further implications for (subseasonal) predictions of the tropospheric and mesospheric circulations.
Sea Surface Energy Fluxes' Response to the Quasi‐Biweekly Oscillation: A Case Study in the South China Sea
Authors: Qi Murong , Han Bo , Yang Qinghua , Wu Renhao , Liu Changwei , Zhang Gong , Zhang Xiaobo , Zhou Haitao , Chen Jianqiao , Chen Dake
Published: August 2023

The South China Sea (SCS) owns the world's strongest quasi‐biweekly oscillation (QBWO) in boreal summer, but the mechanism is still unclear. This case study summarizes two modes of QBWO over the summer SCS in 2019 by using empirical orthogonal function on the 10–20‐day bandpass‐filtered outgoing longwave radiation fields. The maximum positive irradiance anomalies for the two modes are 90 W m−2. The upward solar and downward longwave radiation anomalies own about 4%–8% of the irradiance magnitude, and the surface upward longwave radiation shows a weak response. Sea surface turbulent heat fluxes' responses to QBWO display different spatial patterns compared to radiation fluxes. Their changes are mainly ascribed to the surface wind in Mode1 and the air‐sea thermal contrast in Mode2. We also discuss the cause and impact of sea surface turbulent heat fluxes on QBWO.
Variation of Hydrogen Energetic Neutral Atom Flux in the Subsolar Magnetosheath as a Function of Solar Cycle
Authors: Sokół J. M. , Starkey M. J. , Dayeh M. A. , Fuselier S. A. , Petrinec S. M. , McComas D. J. , Schwadron N. A. , Szalay J. , Ogasawara K.
Published: September 2023

We analyzed observations of energetic neutral atoms (ENAs) of hydrogen collected from the subsolar magnetosheath by the Interstellar Boundary Explorer (IBEX) in Solar Cycle 24. We looked for long‐term solar activity‐related variation and correlation between the H ENA flux and the solar wind parameters. The H ENA flux in the energy range from 0.5 to 6 keV increased during the maximum and declining phases of the solar cycle. This H ENA flux increase happened during a period of enhanced solar wind dynamic pressure observed at 1 au. We study the relation between the ENA flux and solar wind pressure in the subsolar magnetosheath and conclude that the increase in the ENA flux is a consequence of enhanced ENA production. It is because of the increased solar wind pressure during the declining phase of the solar activity that moved the subsolar magnetosheath earthward into a region of denser hydrogen geocorona, thus increasing the H ENA production. Furthermore, we report a positive correlation between the H ENA flux in the subsolar magnetosheath and the solar wind pressure for the first four energy steps observed by IBEX‐Hi and a decrease for the highest energy acquired, which indicates the presence of a different parent ion population for those ENAs from that of the core solar wind. Our study also shows that the ENA flux correlates differently with the solar wind density and speed as a function of increasing energy of ENAs.
Paleoseismological Constraints on the Anghiari Normal Fault (Northern Apennines, Italy) and Potential Implications for the Activity of the Altotiberina Low‐Angle Normal Fault
Authors: Testa A. , Boncio P. , Baize S. , Mirabella F. , Pucci S. , Pace B. , Riesner M. , Pauselli C. , Ercoli M. , Benedetti L. , Di Chiara A. , Civico R.
Published: September 2023

The NE‐dipping Anghiari normal fault, bounding to the west the Sansepolcro basin in the Upper Tiber Valley (northern Apennines), is thought to be a synthetic splay of the Altotiberina low‐angle normal fault (LANF), an active ENE‐dipping extensional detachment whose seismogenic behavior is debated. In order to assess the Anghiari fault capability to break the surface during strong earthquakes and be the source of historical earthquakes, we acquired high resolution topographic data, performed field survey and geophysical investigations (Seismic reflection, Ground Penetrating Radar, Electrical Resistivity Tomography) and dug three paleoseismological trenches across different fault sections of the Anghiari fault. The acquired data reveal for the first time the Late Pleistocene to historical activity of the Anghiari fault, constraining the age of seven paleo‐earthquakes over the last 25 ka, the youngest of which is comparable with one of the poorly constrained historical earthquakes of the Sansepolcro basin. The yielded slip rate is >0.2 mm/yr averaged over the last 25 ka and the recurrence interval is about 2,500–3,200 years. An analysis of the anisotropy of the magnetic susceptibility performed in one of the paleoseismological trenches revealed an extensional stress field, continuously acting during the sedimentation of the entire trenched stratigraphy. Our results confirm the ability of the Anghiari fault to generate surface faulting earthquakes. In addition, if the Anghiari fault does sole at depth into the Altotiberina low‐angle normal fault, this LANF could also be seismogenic and generateM > 6.
Crustal‐Scale Seismic Reflection Profiling Constrains How the Paleo‐Asian Ocean Was Closed
Authors: Tan Xiao‐Miao , Zhou Jian‐Bo , Deng Xiao‐Fan , Wang Hai‐Yan , Hou He‐Sheng , Li Hui‐Lin , Qi Rui , Xie Fan , Gao Rui
Published: September 2023

The Central Asian Orogenic Belt (CAOB) is the most significant accretionary orogenic belt since the Phanerozoic and the most ideal site for studying continental growth evolution processes. A 460‐km‐long high‐resolution crustal‐scale seismic reflection study was conducted across the eastern CAOB in North‐Central China to constrain the closure mode and location of the Paleo‐Asian Ocean, that is, the previous ocean of the CAOB. The resultant seismic reflection profile revealed opposite‐dipping reflectors in the northern and southern parts of the profile, which converge at the profile center to form an inverted U‐shaped reflector pattern near the crust–mantle transition zone beneath the Solonker Suture. The dipping reflectors represent bidirectional fossil subduction zones sloping to the north and south, and the convergence reflector pattern represents the ocean closure location. Integration of these results with available geological data facilitated model construction whereby Paleo‐Asian Ocean closure was accomplished by divergent subduction of the Paleo‐Asian oceanic plate, with northward subduction beneath the southern margin of the Mongolian Block and southward subduction beneath the northern margin of the North China Craton. The oceanic lithosphere contracted and deformed, yielding the observed inverted U‐shaped reflector pattern, representing Paleo‐Asian Ocean closure. This subsurface location lies beneath the Solonker Suture surface exposure, suggesting that this suture marks the ocean closure location, rather than the previously proposed Hegenshan–Heihe Suture to the north or Xar Moron Suture to the south. Our study suggests that divergently dipping subduction and associated accretion and magmatism may constitute the primary continental growth mode for accretionary‐type orogens.
Light People: Professor Fan Wang
Authors: Guo Siqiu
Published: September 2023

Editorial

My first encounter with Prof. Fan Wang left a profound impression on me. I felt that he was exactly the gentle and courteous scholar depicted in books, well-read in poetry and literature, and exceptionally talented. Through my interactions with Prof. Fan Wang, I deeply sensed his passion for academia and pursuit of knowledge, as well as his warm hospitality, kindness, and gentle demeanor.

His thinking is profound and broad, capable of examining issues from various perspectives, and providing inspiration. Prof. Fan Wang is a leading young scientist who actively engages in various academic activities, concerns himself with cutting-edge technological issues, and dedicates himself to overcoming research challenges that can drive advancements and developments in optoelectronics, biophotonics and nanomaterials.

Prof. Fan Wang’s talent, knowledge, character, and sense of family responsibility all fill me with admiration and respect. Now, let’s step into the world of Light People Prof. Fan Wang and together, appreciate the brilliance of his carefree and extraordinary life.
Intestinal microbiota links to allograft stability after lung transplantation: a prospective cohort study
Authors: Wu Junqi , Li Chongwu , Gao Peigen , Zhang Chenhong , Zhang Pei , Zhang Lei , Dai Chenyang , Zhang Kunpeng , Shi Bowen , Liu Mengyang , Zheng Junmeng , Pan Bo , Chen Zhan , Zhang Chao , Liao Wanqing , Pan Weihua , Fang Wenjie , Chen Chang
Published: September 2023

Whether the alternated microbiota in the gut contribute to the risk of allograft rejection (AR) and pulmonary infection (PI) in the setting of lung transplant recipients (LTRs) remains unexplored. A prospective multicenter cohort of LTRs was identified in the four lung transplant centers. Paired fecal and serum specimens were collected and divided into AR, PI, and event-free (EF) groups according to the diagnosis at sampling. Fecal samples were determined by metagenomic sequencing. And metabolites and cytokines were detected in the paired serum to analyze the potential effect of the altered microbiota community. In total, we analyzed 146 paired samples (AR = 25, PI = 43, and EF = 78). Notably, we found that the gut microbiome of AR followed a major depletion pattern with decreased 487 species and compositional diversity. Further multi-omics analysis showed depleted serum metabolites and increased inflammatory cytokines in AR and PI. Bacteroides uniformis, which declined in AR (2.4% vs 0.6%) and was negatively associated with serum IL-1β and IL-12, was identified as a driven specie in the network of gut microbiome of EF. Functionally, the EF specimens were abundant in probiotics related to mannose and cationic antimicrobial peptide metabolism. Furthermore, a support-vector machine classifier based on microbiome, metabolome, and clinical parameters highly predicted AR (AUPRC = 0.801) and PI (AUPRC = 0.855), whereby the microbiome dataset showed a particularly high diagnostic power. In conclusion, a disruptive gut microbiota showed a significant association with allograft rejection and infection and with systemic cytokines and metabolites in LTRs.
The Neurod1/4-Ntrk3-Src pathway regulates gonadotrope cell adhesion and motility
Authors: Le Ciclé Charles , Pacini Vincent , Rama Nicolas , Tauszig-Delamasure Servane , Airaud Eloïse , Petit Florence , de Beco Simon , Cohen-Tannoudji Joëlle , L’hôte David
Published: September 2023

Pituitary gonadotrope cells are essential for the endocrine regulation of reproduction in vertebrates. These cells emerge early during embryogenesis, colonize the pituitary glands and organize in tridimensional networks, which are believed to be crucial to ensure proper regulation of fertility. However, the molecular mechanisms regulating the organization of gonadotrope cell population during embryogenesis remain poorly understood. In this work, we characterized the target genes of NEUROD1 and NEUROD4 transcription factors in the immature gonadotrope αT3-1 cell model by in silico functional genomic analyses. We demonstrated that NEUROD1/4 regulate genes belonging to the focal adhesion pathway. Using CRISPR/Cas9 knock-out approaches, we established a double NEUROD1/4 knock-out αT3-1 cell model and demonstrated that NEUROD1/4 regulate cell adhesion and cell motility. We then characterized, by immuno-fluorescence, focal adhesion number and signaling in the context of NEUROD1/4 insufficiency. We demonstrated that NEUROD1/4 knock-out leads to an increase in the number of focal adhesions associated with signaling abnormalities implicating the c-Src kinase. We further showed that the neurotrophin tyrosine kinase receptor 3 NTRK3, a target of NEUROD1/4, interacts physically with c-Src. Furthermore, using motility rescue experiments and time-lapse video microscopy, we demonstrated that NTRK3 is a major regulator of gonadotrope cell motility. Finally, using a Ntrk3 knock-out mouse model, we showed that NTRK3 regulates gonadotrope cells positioning in the developing pituitary, in vivo. Altogether our study demonstrates that the Neurod1/4-Ntrk3-cSrc pathway is a major actor of gonadotrope cell mobility, and thus provides new insights in the regulation of gonadotrope cell organization within the pituitary gland.
Knockdown of KIF15 suppresses proliferation of prostate cancer cells and induces apoptosis through PI3K/Akt signaling pathway
Authors: Bi Hai , Hou Xiaofei , Shen Qiyang , Liu Zenan , Zhu Xuehua , Ma Lulin , Lu Jian
Published: September 2023

Prostate cancer is one of the most common malignancies in men, which has been considered a public health threat. KIF15 is a kind of driver protein, and its abnormal expression is closely related to the occurrence and development of malignant tumors. The purpose of the study was to explore the significance and role of KIF15 in prostate cancer and to show some potential value for prostate cancer. Immunohistochemistry analysis showed that KIF15 was highly expressed in prostate cancer tissues, which was also positively correlated with T Infiltrate. The loss-of-function and gain-of-function assays based on prostate cancer cells indicated that the change in KIF15 expression could significantly affect cell proliferation, tumorigenesis, migration, and cell apoptosis. The inhibition of prostate cancer development by KIF15 knockdown was also assured in vivo. The Human Apoptosis Antibody Array showed that CD40L, cytoC, DR6, and p21 were up-regulated upon KIF15 knockdown, while IGF-I and Survivin were down-regulated. Moreover, the involvement of the PI3K/Akt pathway in the KIF15-mediated regulation of prostate cancer was preliminarily proved. In summary, KIF15 was identified to play an important role in the development or biological progress of prostate cancer and is considered to possess the potential to be used as a therapeutic target.
Transposable elements as tissue-specific enhancers in cancers of endodermal lineage
Authors: Karttunen Konsta , Patel Divyesh , Xia Jihan , Fei Liangru , Palin Kimmo , Aaltonen Lauri , Sahu Biswajyoti
Published: September 2023

Transposable elements (TE) are repetitive genomic elements that harbor binding sites for human transcription factors (TF). A regulatory role for TEs has been suggested in embryonal development and diseases such as cancer but systematic investigation of their functions has been limited by their widespread silencing in the genome. Here, we utilize unbiased massively parallel reporter assay data using a whole human genome library to identify TEs with functional enhancer activity in two human cancer types of endodermal lineage, colorectal and liver cancers. We show that the identified TE enhancers are characterized by genomic features associated with active enhancers, such as epigenetic marks and TF binding. Importantly, we identify distinct TE subfamilies that function as tissue-specific enhancers, namely MER11- and LTR12-elements in colon and liver cancers, respectively. These elements are bound by distinct TFs in each cell type, and they have predicted associations to differentially expressed genes. In conclusion, these data demonstrate how different cancer types can utilize distinct TEs as tissue-specific enhancers, paving the way for comprehensive understanding of the role of TEs as bona fide enhancers in the cancer genomes.
Acquired miR-142 deficit in leukemic stem cells suffices to drive chronic myeloid leukemia into blast crisis
Authors: Zhang Bin , Zhao Dandan , Chen Fang , Frankhouser David , Wang Huafeng , Pathak Khyatiben V. , Dong Lei , Torres Anakaren , Garcia-Mansfield Krystine , Zhang Yi , Hoang Dinh Hoa , Chen Min-Hsuan , Tao Shu , Cho Hyejin , Liang Yong , Perrotti Danilo , Branciamore Sergio , Rockne Russell , Wu Xiwei , Ghoda Lucy , Li Ling , Jin Jie , Chen Jianjun , Yu Jianhua , Caligiuri Michael A. , Kuo Ya-Huei , Boldin Mark , Su Rui , Swiderski Piotr , Kortylewski Marcin , Pirrotte Patrick , Nguyen Le Xuan Truong , Marcucci Guido
Published: September 2023

The mechanisms underlying the transformation of chronic myeloid leukemia (CML) from chronic phase (CP) to blast crisis (BC) are not fully elucidated. Here, we show lower levels of miR-142 in CD34+CD38− blasts from BC CML patients than in those from CP CML patients, suggesting that miR-142 deficit is implicated in BC evolution. Thus, we create miR-142 knockout CML (i.e., miR-142−/−BCR-ABL) mice, which develop BC and die sooner than miR-142 wt CML (i.e., miR-142+/+BCR-ABL) mice, which instead remain in CP CML. Leukemic stem cells (LSCs) from miR-142−/−BCR-ABL mice recapitulate the BC phenotype in congenic recipients, supporting LSC transformation by miR-142 deficit. State-transition and mutual information analyses of “bulk” and single cell RNA-seq data, metabolomic profiling and functional metabolic assays identify enhanced fatty acid β-oxidation, oxidative phosphorylation and mitochondrial fusion in LSCs as key steps in miR-142-driven BC evolution. A synthetic CpG-miR-142 mimic oligodeoxynucleotide rescues the BC phenotype in miR-142−/−BCR-ABL mice and patient-derived xenografts.
Pressure evolution of electron dynamics in the superconducting kagome metal CsV3Sb5
Authors: Wenzel Maxim , Tsirlin Alexander A. , Capitani Francesco , Chan Yuk T. , Ortiz Brenden R. , Wilson Stephen D. , Dressel Martin , Uykur Ece
Published: September 2023

The coexistence of the charge-density wave (CDW) and superconducting phases and their tunability under external pressure remains one of the key points in understanding the electronic structure of AV3Sb5 (A = K, Rb, Cs) kagome metals. Here, we employ synchrotron-based infrared spectroscopy assisted by density-functional calculations to study the pressure evolution of the electronic structure at room temperature up to 17 GPa experimentally. The optical spectrum of CsV3Sb5 is characterized by the presence of localized carriers seen as a broad peak at finite frequencies in addition to the conventional metallic Drude response. The non-monotonic pressure dependence of this low-energy peak reflects the re-entrant behavior of superconductivity and may be interpreted in terms of electron-phonon coupling, varying with the growth and shrinkage of the Fermi surface under pressure. Moreover, drastic modifications in the low-energy interband absorptions are observed upon the suppression of CDW. These changes are related to the upward shift of the Sb2 px + py band that eliminates part of the Fermi surface around the M-point, whereas band saddle points do not move significantly. These observations shed new light on the mixed electronic and lattice origin of the CDW in CsV3Sb5.
Time of day and sleep effects on motor acquisition and consolidation
Authors: Truong Charlène , Ruffino Célia , Gaveau Jérémie , White Olivier , Hilt Pauline M. , Papaxanthis Charalambos
Published: September 2023

We investigated the influence of the time-of-day and sleep on skill acquisition (i.e., skill improvement immediately after a training-session) and consolidation (i.e., skill retention after a time interval including sleep). Three groups were trained at 10 a.m. (G10am), 3 p.m. (G3pm), or 8 p.m. (G8pm) on a finger-tapping task. We recorded the skill (i.e., the ratio between movement duration and accuracy) before and immediately after the training to evaluate acquisition, and after 24 h to measure consolidation. We did not observe any difference in acquisition according to the time of the day. Interestingly, we found a performance improvement 24 h after the evening training (G8pm), while the morning (G10am) and the afternoon (G3pm) groups deteriorated and stabilized their performance, respectively. Furthermore, two control experiments (G8awake and G8sleep) supported the idea that a night of sleep contributes to the skill consolidation of the evening group. These results show a consolidation when the training is carried out in the evening, close to sleep, and forgetting when the training is carried out in the morning, away from sleep. This finding may have an important impact on the planning of training programs in sports, clinical, or experimental domains.
“You did incredibly well!”: teachers’ inflated praise can make children from low-SES backgrounds seem less smart (but more hardworking)
Authors: Schoneveld Emiel , Brummelman Eddie
Published: September 2023

Can teachers’ inflated praise make children from low socioeconomic status (SES) backgrounds seem less smart? We conducted two preregistered experiments to address this question. We used hypothetical scenarios to ensure experimental control. An experiment with primary school teachers (N = 106, ages 21–63) showed that when a child from a low-SES (vs. high-SES) background succeeded in school, teachers attributed this success more to hard work and delivered more inflated praise (e.g., “You did incredibly well!”) but less modest praise (e.g., “You did well!”). An experiment with primary school children (N = 63, ages 10–13) showed that when children learned that another child received inflated praise (while an equally performing classmate received modest praise or no praise), they perceived this child as less smart but more hardworking. These studies provide converging evidence that teachers’ inflated praise, although well-intentioned, can make children from low-SES backgrounds seem less smart, thereby reinforcing negative stereotypes about these children’s academic abilities.
Sub-arc mantle fugacity shifted by sediment recycling across the Great Oxidation Event
Authors: Moreira Hugo , Storey Craig , Bruand Emilie , Darling James , Fowler Mike , Cotte Marine , Villalobos-Portillo Edgar E. , Parat Fleurice , Seixas Luís , Philippot Pascal , Dhuime Bruno
Published: August 2023

The chemical exchange between the atmosphere, crust and mantle depends on sediment recycling via subduction. However, it remains unclear how atmospherically modified sediment may affect mantle oxygen fugacity through time. The Great Oxidation Event, among the most important atmospheric changes on Earth, offers an opportunity to investigate changes in magmatism related to surface–mantle interactions. Here we use sulfur K-edge micro X-ray absorption near-edge structure spectroscopy to measure the relative abundances of S6+, S4+ and S2− state in apatite inclusions hosted in 2.4–2.1-billion-year-old igneous zircons from the Mineiro Belt, Brazil. The host magmas record intracrustal melting of juvenile crust and the involvement of recycled sediments in the sub-arc mantle wedge. Unaltered apatite inclusions reveal a change from reduced to more oxidized magmas from pre- to post-Great Oxidation Event during the early Proterozoic. We argue that this change is a direct result of deep subduction of oxidized sediments and thus evidence of mantle–atmosphere interaction across the Great Oxidation Event. This suggests that the onset of sediment recycling in the Archaean provided atmospheric access to the mantle, and early ‘whiffs’ of oxygen may have already contributed to a localized increase of calc-alkaline magmatism and related ore deposits on Earth.
Genomic analysis of phylogroup D Escherichia coli strains using novel de-novo reference-based guided assembly
Authors: Aswal Manisha , Singhal Neelja , Kumar Manish
Published: September 2023

Escherichia coli are highly diverse bacteria with different pathogenic types, serotypes and phylogenetic types/phylotypes. In recent years, infections with E. coli have increased worldwide and so has the emergence of antibiotic resistant strains. In the present study we have assembled, annotated and analysed genome sequences of three strains of the phylogroup D of E. coli. These strains were isolated from the river Yamuna, a prominent anthropogenic urban river of northern India. These strains showed varied antibiotic susceptibilities, one was susceptible to all the antibiotics tested except ampicillin while of the other two, one was multi-β-lactam resistant and the other was multi-drug resistant (resistant to multiple β-lactams, fluoroquinolones and kanamycin). The short-sequence reads were assembled into contigs using the de-novo approach and further, scaffolding of contigs was performed by using the best reference genome for a particular isolate which resulted in a significant increase in the N50 value of each assembly. The bioinformatics assembly approach used in this study could be easily applied to study other bacterial genomes.
Pre-neuronal processing of haptic sensory cues via dispersive high-frequency vibrational modes
Authors: Ding Yu , Vlasov Yurii
Published: September 2023

Sense of touch is one of the major perception channels. Neural coding of object textures conveyed by rodents’ whiskers has been a model to study early stages of haptic information uptake. While high-precision spike timing has been observed during whisker sweeping across textured surfaces, the exact nature of whisker micromotions that spikes encode remains elusive. Here, we discovered that a single micro-collision of a whisker with surface features generates vibrational eigenmodes spanning frequencies up to 10 kHz. While propagating along the whisker, these high-frequency modes can carry up to 80% of shockwave energy, exhibit 100× smaller damping ratio, and arrive at the follicle 10× faster than low frequency components. The mechano-transduction of these energy bursts into time-sequenced population spike trains may generate temporally unique “bar code” with ultra-high information capacity. This hypothesis of pre-neuronal processing of haptic signals based on dispersive temporal separation of the vibrational modal frequencies can shed light on neural coding of haptic signals in many whisker-like sensory organs across the animal world as well as in texture perception in primate’s glabrous skin.
Non-chemotherapy adjuvant agents in TP53 mutant Ewing sarcoma
Authors: Kim Jin-Ah , Crawford Kenneth A. , Spada Piero A. , Martin Leah R. , Zhang Jiaqi , Wong Rain , Reid Joel M. , Stewart Clinton F. , Frank Timothy M. , Liu Qianqian , Michalek Joel E. , Keller Charles
Published: September 2023

Ewing sarcoma (EWS) is a malignant tumor arising in bone or soft tissue that occurs in adolescent and young adult patients as well as adults later in life. Although non-metastatic EWS is typically responsive to treatment when newly diagnosed, relapsed cases have an unmet need for which no standard treatment approach exists. Recent phase III clinical trials for EWS comparing 7 vs 5 chemotherapy drugs have failed to improve survival. To extend the durability of remission for EWS, we investigated 3 non-chemotherapy adjuvant therapy drug candidates to be combined with chemotherapy. The efficacy of these adjuvant drugs was investigated via anchorage-dependent growth assays, anchorage-independent soft-agar colony formation assays and EWS xenograft mouse models. Enoxacin and entinostat were the most effective adjuvant drug in both long-term in vitro and in vivo adjuvant studies. In the context that enoxacin is an FDA-approved antibiotic, and that entinostat is an investigational agent not yet FDA-approved, we propose enoxacin as an adjuvant drug for further preclinical and clinical investigation in EWS patients.
Identification of growth years for Puerariae Thomsonii Radix based on hyperspectral imaging technology and deep learning algorithm
Authors: Zhang Lei , Guan Yu , Wang Ni , Ge Fei , Zhang Yan , Zhao Yuping
Published: August 2023

Puerariae Thomsonii Radix (PTR) is not only widely used in disease prevention and treatment but is also an important raw material as a source of starch and other food. The growth years of PTR are closely related to its quality. The rapid and nondestructive identification of growth year is essential for the quality control of PTR and other traditional Chinese medicines. In this study, we proposed a convolutional neural network (CNN)-based classification framework in conjunction with hyperspectral imaging (HSI) technology for the rapid identification of the growth years of PTRs. Traditional treatment methods (i.e., multiplicative scatter correction, standard normal variate, and Savitzky-Golay smoothing) combined with machine learning algorithms (i.e., random forest, logistic regression, naive Bayes, and eXtreme gradient boost) were used as baseline models. Among them, the F1-score of CNN-based models based on PTRs’ outer surfaces was over 90%, outperforming all the other baseline models. These results showed that it was feasible to use a deep learning algorithm in conjunction with HSI technology to identify the growth years of PTR. This method provides a fast, nondestructive, and simple method of identifying the growth years of PTR. It can be easily applied to other scenarios, such as for the identification of the locality or years of growth for other traditional Chinese herbs.
Homogentisate 1,2-dioxygenase (HGD) gene variants in young Egyptian patients with alkaptonuria
Authors: Abdelkhalek Zeinab S. , Mahmoud Iman G. , Omair Heba , Abdulhay Mohamed , Elmonem Mohamed A.
Published: September 2023

Alkaptonuria (AKU) is a rare autosomal recessive metabolic disorder caused by pathogenic variants in the homogentisate 1,2-dioxygenase (HGD) gene. This leads to a deficient HGD enzyme with the consequent accumulation of homogentisic acid (HGA) in different tissues causing complications in various organs, particularly in joints, heart valves and kidneys. The genetic basis of AKU in Egypt is completely unknown. We evaluated the clinical and genetic spectrum of six pediatric and adolescents AKU patients from four unrelated Egyptian families. All probands had a high level of HGA in urine by qualitative GC/MS before genetic confirmation by Sanger sequencing. Recruited AKU patients were four females and two males (median age 13 years). We identified four different pathogenic missense variants within HGD gene. Detected variants included a novel variant c.1079G > T;p.(Gly360Val) and three recurrent variants; c.1078G > C;p.(Gly360Arg), c.808G > A;p.(Gly270Arg) and c.473C > T;p.(Pro158Leu). All identified variants were properly segregating in the four families consistent with autosomal recessive inheritance. In this study, we reported the phenotypic and genotypic spectrum of alkaptonuria for the first time in Egypt. We further enriched the HGD-variant database with another novel pathogenic variant. The recent availability of nitisinone may promote the need for genetic confirmation at younger ages to start therapy earlier and prevent serious complications.
Analysis of flow-induced transcriptional response and cell alignment of different sources of endothelial cells used in vascular tissue engineering
Authors: Rojas-González Diana M. , Babendreyer Aaron , Ludwig Andreas , Mela Petra
Published: September 2023

Endothelialization of tissue-engineered vascular grafts has proven crucial for implant functionality and thus clinical outcome, however, the choice of endothelial cells (ECs) is often driven by availability rather than by the type of vessel to be replaced. In this work we studied the response to flow of different human ECs with the aim of examining whether their response in vitro is dictated by their original in vivo conditions. Arterial, venous, and microvascular ECs were cultured under shear stress (SS) of 0, 0.3, 3, 1, 10, and 30 dyne/cm2 for 24 h. Regulation of flow-induced marker KLF2 was similar across the different ECs. Upregulation of anti-thrombotic markers, TM and TPA, was mainly seen at higher SS. Cell elongation and alignment was observed for the different ECs at 10 and 30 dyne/cm2 while at lower SS cells maintained a random orientation. Downregulation of pro-inflammatory factors SELE, IL8, and VCAM1 and up-regulation of anti-oxidant markers NQO1 and HO1 was present even at SS for which cell alignment was not observed. Our results evidenced similarities in the response to flow among the different ECs, suggesting that the maintenance of the resting state in vitro is not dictated by the SS typical of the tissue of origin and that absence of flow-induced cell orientation does not necessarily correlate with a pro-inflammatory state of the ECs. These results support the use of ECs from easily accessible sources for in vitro vascular tissue engineering independently from the target vessel.