bluuebunny/crossref_metadata_2025 huggingface dataset:
A Systematic Review of the Influence of Bovine Colostrum Supplementation on Leaky Gut Syndrome in Athletes: Diagnostic Biomarkers and Future Directions
Authors: Dziewiecka Hanna , Buttar Harpal S. , Kasperska Anna , Ostapiuk-Karolczuk Joanna , Domagalska Małgorzata , Cichoń Justyna , Skarpańska-Stejnborn Anna
Published: June 2022

Background: Bovine colostrum (BC) contains a myriad of bioactive molecules that are renowned for possessing unique medicinal benefits in children and adults, and BC supplements are considered safe and cost-effective options to manage/prevent the incidence of upper respiratory tract infections and gut-related problems in athletes. In this review, we will try to answer the question: How will BC supplementation ameliorate gut permeability problems among athletes? Methods: Literature searches were performed using PRISMA guidance to identify studies assessing the influence of BC supplements on gut permeability. Studies were selected using four databases: PubMed, Web of Science, Scopus, and EBSCO, and a total number of 60 articles were retrieved by using appropriate keywords. Results: Nine studies were selected that met the eligibility criteria for this review. The data analysis revealed that vigorous exercise profoundly increases intestinal permeability, and BC supplementation helps to reverse gut permeability in athletes. Conclusion: BC supplementation may be highly beneficial in improving gut permeability in athletes. However, well-designed, placebo-controlled, and randomized studies are needed to evaluate the long-term safety and efficacy and to determine the optimal dose schedules of BC supplementation in high-performance athletes.
The Association of Dietary Fiber Intake in Three Meals with All-Cause and Disease-Specific Mortality among Adults: The U.S. National Health and Nutrition Examination Survey, 2003–2014
Authors: Qi Jiayue , Gao Jian , Zhang Yuntao , Hou Wanying , Han Tianshu , Sun Changhao
Published: June 2022

The timing of food intake can significantly alter the body’s metabolism of nutrient intake and affect the occurrence of chronic diseases. However, whether and how the intake time of dietary fiber could influence mortality risks is largely unknown. This study aims to reveal the association between total dietary fiber intake and fiber intake at different times with all-cause, cancer, and cardiovascular disease (CVD) mortality rates. A total of 31,164 adults who enrolled in the National Health and Nutrition Examination Survey from 2003 to 2014 are included in this study. Dietary fiber intake was measured using 2-day, 24 h dietary recall. The main exposures in this study were the intake of dietary fiber at breakfast, lunch, and dinner via regression analysis of the residual method. The main outcomes were the all-cause, cancer, and CVD mortality rates. Cox proportional hazards regression models were used to evaluate the survival relationship between dietary fiber intake at different times and mortality rates. Among the 31,164 adults, 2915 deaths, including 631 deaths due to cancer and 836 deaths due to CVD, were documented. Firstly, after adjusting for potential confounders, compared to the participants in the lowest quintile of total dietary fiber intake, the participants in the highest quintile of fiber intake had lower all-cause (HR = 0.686, 95% CI: 0.589–0.799, p for trend <0.001) and cancer (HR = 0.606, 95% CI: 0.446–0.824, p for trend = 0.015) mortality risks. Secondly, compared to the participants in the lowest quintile of dietary fiber intake at dinner, the participants in the highest quintile of fiber intake had lower all-cause (HR = 0.796, 95% CI: 0.668–0.949, p for trend = 0.009) and cancer (HR = 0.564, 95% CI: 0.388–0.822, p for trend = 0.005) mortality risks. Furthermore, equivalently replacing each standard deviation of dietary fiber consumed at breakfast with that at dinner was associated with lower cancer mortality risks (HR = 0.846, 95% CI: 0.747–0.958). In conclusion, this study demonstrates that, in the NHANES (2003–2014) cohort, to reduce all-cause and cancer mortality risks, the optimal dietary fiber intake time is in the evening.
Impact of Zinc Transport Mechanisms on Embryonic and Brain Development
Authors: Willekens Jeremy , Runnels Loren W.
Published: June 2022

The trace element zinc (Zn) binds to over ten percent of proteins in eukaryotic cells. Zn flexible chemistry allows it to regulate the activity of hundreds of enzymes and influence scores of metabolic processes in cells throughout the body. Deficiency of Zn in humans has a profound effect on development and in adults later in life, particularly in the brain, where Zn deficiency is linked to several neurological disorders. In this review, we will summarize the importance of Zn during development through a description of the outcomes of both genetic and early dietary Zn deficiency, focusing on the pathological consequences on the whole body and brain. The epidemiology and the symptomology of Zn deficiency in humans will be described, including the most studied inherited Zn deficiency disease, Acrodermatitis enteropathica. In addition, we will give an overview of the different forms and animal models of Zn deficiency, as well as the 24 Zn transporters, distributed into two families: the ZIPs and the ZnTs, which control the balance of Zn throughout the body. Lastly, we will describe the TRPM7 ion channel, which was recently shown to contribute to intestinal Zn absorption and has its own significant impact on early embryonic development.
Quercetin-Rich Extracts from Onions (Allium cepa) Play Potent Cytotoxicity on Adrenocortical Carcinoma Cell Lines, and Quercetin Induces Important Anticancer Properties
Authors: Veiga Alan A. , Irioda Ana Carolina , Mogharbel Bassam F. , Bonatto Sandro J. R. , Souza Lauro M.
Published: June 2022

Adrenocortical carcinoma (ACC) is a rare subtype of cancer, with a poor prognosis in children and adults. Mitotane is the only approved adrenolytic drug for the treatment of ACC, which has controversies regarding its efficacy and side effects on patients. Onion (Allium cepa), a worldwide consumed food, is associated with many health benefits. Along with its glycosides, the flavonoid quercetin is abundant in onions. After evaluating the cytotoxicity of A. cepa extracts on adrenocortical carcinoma cell line (H295R), the rich quercetin fractions had better results. Then, we aimed to compare the quercetin vs. mitotane effectiveness, using adrenocortical carcinoma cell lines H295R and SW-13. Quercetin showed a higher cytotoxicity response on both cancerous cell lines after 10 µM concentration, while mitotane only after 30 µM. Cell cycle dynamics were altered upon quercetin treatments, with G2 phase increase with 30 µM of quercetin on H295R cell line and G1 arrest on SW-13 cell line with 15 µM. Early and late apoptosis, alongside intracellular calcium, were increased on SW-13 treated with 30 µM of quercetin, and ROS rates were reduced by quercetin on H295R. Therefore, quercetin-rich onions have the potential to be a natural source of anticancer agents for adrenocortical carcinoma.
Ginsenoside Rh3 Inhibits Lung Cancer Metastasis by Targeting Extracellular Signal-Regulated Kinase: A Network Pharmacology Study
Authors: Xue Xiaodan , Liu Yannan , Qu Linlin , Fan Cuiying , Ma Xiaoxuan , Ouyang Pingkai , Fan Daidi
Published: June 2022

Lung cancer has a high mortality rate and is very common. One of the main reasons for the poor prognosis of patients with lung cancer is the high incidence of metastasis. Ginsenoside Rh3, a rare ginsenoside extracted from Panax notoginseng, exhibits excellent anti-inflammatory and anti-tumor effects. Nonetheless, the inhibitory potential of Rh3 against lung cancer remains unknown. The target genes of Rh3 were screened by the PharmMapper database; the proliferation of lung cancer cells was detected by MTT assay; the migration and invasion of cells were detected by the Transwell method; and the expression of extracellular signal-regulated kinase (ERK) and EMT-related proteins in vivo and in vitro were detected by Western blotting. In addition, we established a lung metastasis model in nude mice using A549 cells to assess the effect of Rh3 on NSCLC tumor metastasis in vivo. Our findings suggest that Rh3 significantly inhibited lung cancer metastasis both in vivo and in vitro. It was determined by flow cytometry analysis that Rh3 notably inhibited cell proliferation by blocking the G1 phase. In addition, Rh3 inhibited metastasis in lung cancer cells and regulated the expression of metastasis-related proteins under hypoxia. Mechanistic studies suggested that Rh3 targeted ERK to inhibit lung cancer metastasis. The ERK inhibitor U0126 or siRNA-mediated knockdown of ERK had an enhanced effect on Rh3’s ability to inhibit lung cancer metastasis. The studies revealed that the inhibitory effect of Rh3 on the metastatic ability of lung cancer cells may be supported by ERK-related signaling pathways.
Gene Therapy for Mitochondrial Diseases: Current Status and Future Perspective
Authors: Di Donfrancesco Alessia , Massaro Giulia , Di Meo Ivano , Tiranti Valeria , Bottani Emanuela , Brunetti Dario
Published: June 2022

Mitochondrial diseases (MDs) are a group of severe genetic disorders caused by mutations in the nuclear or mitochondrial genome encoding proteins involved in the oxidative phosphorylation (OXPHOS) system. MDs have a wide range of symptoms, ranging from organ-specific to multisystemic dysfunctions, with different clinical outcomes. The lack of natural history information, the limits of currently available preclinical models, and the wide range of phenotypic presentations seen in MD patients have all hampered the development of effective therapies. The growing number of pre-clinical and clinical trials over the last decade has shown that gene therapy is a viable precision medicine option for treating MD. However, several obstacles must be overcome, including vector design, targeted tissue tropism and efficient delivery, transgene expression, and immunotoxicity. This manuscript offers a comprehensive overview of the state of the art of gene therapy in MD, addressing the main challenges, the most feasible solutions, and the future perspectives of the field.
Safety Challenges and Application Strategies for the Use of Dendrimers in Medicine
Authors: Li Xiang , Naeem Abid , Xiao Shanghua , Hu Lei , Zhang Jing , Zheng Qin
Published: June 2022

Dendrimers are used for a variety of applications in medicine but, due to their host–guest and entrapment characteristics, are particularly used for the delivery of genes and drugs. However, dendrimers are intrinsically toxic, thus creating a major limitation for their use in biological systems. To reduce such toxicity, biocompatible dendrimers have been designed and synthesized, and surface engineering has been used to create advantageous changes at the periphery of dendrimers. Although dendrimers have been reviewed previously in the literature, there has yet to be a systematic and comprehensive review of the harmful effects of dendrimers. In this review, we describe the routes of dendrimer exposure and their distribution in vivo. Then, we discuss the toxicity of dendrimers at the organ, cellular, and sub-cellular levels. In this review, we also describe how technology can be used to reduce dendrimer toxicity, by changing their size and surface functionalization, how dendrimers can be combined with other materials to generate a composite formulation, and how dendrimers can be used for the diagnosis of disease. Finally, we discuss future challenges, developments, and research directions in developing biocompatible and safe dendrimers for medical purposes.
Unraveling NPR-like Family Genes in Fragaria spp. Facilitated to Identify Putative NPR1 and NPR3/4 Orthologues Participating in Strawberry-Colletotrichum fructicola Interaction
Authors: Bai Yun , Li Ziyi , Zhu Jiajun , Chen Siyu , Dong Chao , Gao Qinghua , Duan Ke
Published: June 2022

The salicylic acid receptor NPR1 (nonexpressor of pathogenesis-related genes) and its paralogues NPR3 and NPR4 are master regulators of plant immunity. Commercial strawberry (Fragaria × ananassa) is a highly valued crop vulnerable to various pathogens. Historic confusions regarding the identity of NPR-like genes have hindered research in strawberry resistance. In this study, the comprehensive identification and phylogenic analysis unraveled this family, harboring 6, 6, 5, and 23 members in F. vesca, F. viridis, F. iinumae, and F. × ananassa, respectively. These genes were clustered into three clades, with each diploid member matching three to five homoalleles in F. × ananassa. Despite the high conservation in terms of gene structure, protein module, and functional residues/motifs/domains, substantial divergence was observed, hinting strawberry NPR proteins probably function in ways somewhat different from Arabidopsis. RT-PCR and RNAseq analysis evidenced the transcriptional responses of FveNPR1 and FxaNPR1a to Colletotrichum fructicola. Extended expression analysis for strawberry NPR-likes helped to us understand how strawberry orchestrate the NPRs-centered defense system against C. fructicola. The cThe current work supports that FveNPR1 and FxaNPR1a, as well as FveNPR31 and FxaNPR31a-c, were putative functional orthologues of AtNPR1 and AtNPR3/4, respectively. These findings set a solid basis for the molecular dissection of biological functions of strawberry NPR-like genes for improving disease resistance.
Viromes of Hungarian Peach Trees Identified by High-Throughput Sequencing of Small RNAs
Authors: Barath Daniel , Jaksa-Czotter Nikoletta , Varga Tunde , Varallyay Eva
Published: June 2022

Peach trees can be infected with viruses and viroids. As we do not have efficient plant protection methods against these pathogens, the prevention of infection is crucial. Fruit trees are maintained by vegetative propagation. Planting material such as certified mother trees and rootstocks should be free from viruses and viroids, and this status has to be regularly checked to prevent infections. We surveyed certified peach trees for the presence of viruses and viroids using small RNA high-throughput sequencing (HTS), an unbiased virus diagnostic method. The results of the bioinformatic analysis of HTS were validated by other molecular methods including RT-PCR, Northern blot hybridization and loop-mediated isothermal amplification (LAMP). We found the presence of plum pox virus and peach latent mosaic viroid (PLMVd) in the vector-free isolator houses, whose presence should be regularly tested. Moreover, we detected frequent infection with recently described viruses such as nectarine stem pitting-associated virus and peach-associated luteovirus (PaLV). During the survey, PLMVd and PaLV were detected for the first time in Hungary. The analysis of the presenting virus variants and possible sources of infection suggests that the source of the viral infection could be the infected propagating material. Our study emphasizes the importance of using sensitive and trustworthy diagnostic techniques to be able to detect viral infections and successfully prevent their spread by propagation material.
Nutrient Composition Analysis of Maize Hybrids Affected by Different Nitrogen Fertilisation Systems
Authors: Bojtor Csaba , Mousavi Seyed Mohammad Nasir , Illés Árpád , Golzardi Farid , Széles Adrienn , Szabó Atala , Nagy János , Marton Csaba L.
Published: June 2022

Maize is one of the most widely used plants in the agricultural industry, and the fields of application of this plant are broad. The experiment was conducted at the Látókép Crop Production Experimental Station of the University of Debrecen, Hungary. Three mid-ripening maize hybrids with different FAO numbers were used in the present study. The effects of different nitrogen supplies were examined as a variable rate of abiotic stress and the interrelationship among the essential nutrients through the nutrient acquisition and partitioning of the different vegetative and generative plant parts. The results showed that NPK application compared to the control treatment (no fertilizer application) increased DM in all tissues of maize, while increasing nitrogen application from 120 to 300 kg ha−1 had no significant effect on this trait. The highest protein content was obtained with the nitrogen application of 120 kg ha−1, and the higher nitrogen fertilizer application had no significant effect on this trait. Seeds and leaves had a maximum zinc and manganese value in terms of nitrogen content (protein). Dry matter was positively correlated with nitrogen, potassium, and manganese content, while the dry matter had a negative correlation with nickel content. In general, to achieve a maximum quantitative and qualitative yield, it is recommended to use NPK fertilizer with a rate of 120 kg ha−1 N for maize cultivation.
The Mutation of Rice MEDIATOR25, OsMED25, Induces Rice Bacterial Blight Resistance through Altering Jasmonate- and Auxin-Signaling
Authors: Suzuki Go , Fukuda Manatsu , Lucob-Agustin Nonawin , Inukai Yoshiaki , Gomi Kenji
Published: June 2022

Rice bacterial blight disease caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most severe diseases of rice. However, the regulatory mechanisms of rice defense against Xoo remain poorly understood. The rice MEDIATOR25, OsMED25—a subunit of the mediator multiprotein complex that acts as a universal adaptor between transcription factors (TFs) and RNA polymerase II—plays an important role in jasmonic acid (JA)-mediated lateral root development in rice. In this study, we found that OsMED25 also plays an important role in JA- and auxin-mediated resistance responses against rice bacterial blight. The osmed25 loss-of-function mutant exhibited high resistance to Xoo. The expression of JA-responsive defense-related genes regulated by OsMYC2, which is a positive TF in JA signaling, was downregulated in osmed25 mutants. Conversely, expression of some OsMYC2-independent JA-responsive defense-related genes was upregulated in osmed25 mutants. Furthermore, OsMED25 interacted with some AUXIN RESPONSE FACTORS (OsARFs) that regulate auxin signaling, whereas the mutated osmed25 protein did not interact with the OsARFs. The expression of auxin-responsive genes was downregulated in osmed25 mutants, and auxin-induced susceptibility to Xoo was not observed in osmed25 mutants. These results indicate that OsMED25 plays an important role in the stable regulation of JA- and auxin-mediated signaling in rice defense response.
Effect of Common Mouthwashes on Mechanical Properties of Suture Materials Used in Dental Surgeries: A Laboratory Experiment
Authors: Abullais Shahabe Saquib , AlOsman Shaker Saleh , AlQahtani Sultan Mansoor , Khan Abdul Ahad , Nahid Rakhshinda , Basheer Sulphi Abdul , Jameel Ahmad Saib
Published: June 2022

Background: Sutures play a pivotal role in promoting healing in any surgical procedure. However, the versatile nature of the oral environment entails additional properties in the suture materials to assure uneventful healing. A surgeon needs to understand these delicate intricacies in the immediate postoperative phase and needs some kind of algorithm in the selection of sutures that suits the different surgical scenarios. Therefore, a study was designed to observe the effect of four different types of mouthwash, viz. Avohex, Aloedent, Parodontax and Betadine, on the mechanical properties of Vicryl (polyglactin 910), PTFE (polytetrafluoroethylene), Prolene (polypropylene) and Mersilk. Methods: A total of 288 suture samples were divided in four equal groups (72 = each group) and used for the experiments. Tensile load, tensile strength as well as percentage elongation of the suture materials were evaluated in the various stated media at 3rd, 7th, 10th and 14th days, which were compared with the controls. The results were analyzed statistically and inferences were drawn. Results: Mersilk in (Avohex, Parodontax and Betadine) solutions showed a statistically significant decrease in tensile load over time. All the sutures had the highest tensile strength in Parodontax and had the lowest in Betadine solution. Vicryl and PTFE presented significant percentage elongation over time as compared to Prolene and Mersilk. Conclusion: The author has given certain recommendations but also states that there is no standard rule for the use of a particular suture material in all clinical situations. However, the selection of the suture material should be based on the type of surgery and preferences of the surgeon.
Active Flexible Films for Food Packaging: A Review
Authors: Azevedo Ana G. , Barros Carolina , Miranda Sónia , Machado Ana Vera , Castro Olga , Silva Bruno , Saraiva Margarida , Silva Ana Sanches , Pastrana Lorenzo , Carneiro Olga Sousa , Cerqueira Miguel A.
Published: June 2022

Active food packaging is a dynamic area where the scientific community and industry have been trying to find new strategies to produce innovative packaging that is economically viable and compatible with conventional production processes. The materials used to develop active packaging can be organized into scavenging and emitting materials, and based on organic and inorganic materials. However, the incorporation of these materials in polymer-based flexible packaging is not always straightforward. The challenges to be faced are mainly related to active agents’ sensitivity to high temperatures or difficulties in dispersing them in the high viscosity polymer matrix. This review provides an overview of methodologies and processes used in the production of active packaging, particularly for the production of active flexible films at the industrial level. The direct incorporation of active agents in polymer films is presented, focusing on the processing conditions and their effect on the active agent, and final application of the packaging material. Moreover, the incorporation of active agents by coating technologies and supercritical impregnation are presented. Finally, the use of carriers to help the incorporation of active agents and several methodologies is discussed. This review aims to guide academic and industrial researchers in the development of active flexible packaging, namely in the selection of the materials, methodologies, and process conditions.
Antiseptic Polymer–Surfactant Complexes with Long-Lasting Activity against SARS-CoV-2
Authors: Molchanov Vyacheslav S. , Shibaev Andrey V. , Karamov Eduard V. , Larichev Viktor F. , Kornilaeva Galina V. , Fedyakina Irina T. , Turgiev Ali S. , Philippova Olga E. , Khokhlov Alexei R.
Published: June 2022

Antiseptic polymer gel–surfactant complexes were prepared by incorporating the low-molecular-weight cationic disinfectant cetylpyridinium chloride into the oppositely charged, slightly cross-linked polymer matrices. Three types of polymers were used: copolymers of acrylamide and sodium 2-acrylamido-2-methylpropane sulfonate; copolymers of acrylamide and sodium methacrylate; copolymers of vinylpyrrolidone and sodium methacrylate. It was shown that the rate of the release of the cationic disinfectant from the oppositely charged polymer gels could be tuned in a fairly broad range by varying the concentration of the disinfectant, the degree of swelling, and degree of cross-linking of the gel and the content/type of anionic repeat units in the polymer matrix. Polymer–surfactant complexes were demonstrated to reduce SARS-CoV-2 titer by seven orders of magnitude in as little as 5 s. The complexes retained strong virucidal activity against SARS-CoV-2 for at least one week.
Research on the Influence of Processing Parameters on the Specific Tensile Strength of FDM Additive Manufactured PET-G and PLA Materials
Authors: Bembenek Michał , Kowalski Łukasz , Kosoń-Schab Agnieszka
Published: June 2022

Fused deposition modeling (FDM) is one of the most accessible additive manufacturing (AM) technologies for processing polymeric materials. It allows processing most of thermoplastic polymers, with polyethylene terephthalate glycol-modified (PET-G) and polylactic acid (PLA). AM parts tend to display anisotropic behavior because of layer-by-layer fabrication and various technological parameters that can be set for 3D print, so it is hard to predict and analyze how the manufactured parts would behave under load. This research presents results of classic tensile strength tests performed on 57 PET-G specimens and 57 PLA specimens manufactured with varying technological parameters such as: printing temperature, print orientation, layer height, and infill percentage. Afterward, a comparative analysis is performed, proposing specific tensile strength (STS) as a benchmark to determine how 3D printed parts strength is varying due to beforementioned parameters, eliminating bias induced by varying weight of specimens. The biggest relative increase of UTS and the biggest relative decrease of STS was noted for variable infill percentage (increasing infill—PLA: 37.27% UTS increase and 30.41% STS decrease; PET-G: 24.42% UTS increase and 37.69% STS decrease). The biggest relative increase of STS between examined parameters was observed for both materials as the printing temperature was increased (27.53% for PLA and 12.69% for PET-G). Similar trends in STS changes were observed for both materials. Obtained data shows which FDM AM parameters are the most important to obtain the biggest UTS of manufactured parts, and those do not overlap with parameters needed to obtain optimal strength-to-weight ratio.
Hepatotropic Peptides Grafted onto Maleimide-Decorated Nanoparticles: Preparation, Characterization and In Vitro Uptake by Human HepaRG Hepatoma Cells
Authors: Brossard Clarisse , Vlach Manuel , Jacquet Lucas , Vène Elise , Dorcet Vincent , Loyer Pascal , Cammas-Marion Sandrine , Lepareur Nicolas
Published: June 2022

We recently demonstrated the strong tropism of George Baker (GB) Virus A (GBVA10-9) and Plasmodium circumsporozoite protein (CPB) derived synthetic peptides towards hepatoma cells. In a first approach, these peptides were covalently bound to poly(benzyl malate) (PMLABe73) and poly(ethylene glycol)-block-PMLABe73 (PEG62-b-PMLABe73) (co)polymers, and corresponding peptide-decorated nanoparticles (NPs) were prepared by nanoprecipitation. We showed that peptide enhanced NPs internalization by hepatoma cells. In the present work, we set up a second strategy to functionalize NPs prepared from PMLABe73 derivates. First, maleimide-functionalized PMLABe73 (Mal-PMLABe73) and PEG62-b-PMLABe73 (Mal-PEG62-b-PMLABe73) were synthesized and corresponding NPs were prepared by nanoprecipitation. Then, peptides (GBVA10-9, CPB and their scramble controls GBVA10-9scr and CPBscr) with a thiol group were engrafted onto the NPs’ maleimide groups using the Michael addition to obtain peptide functionalized NPs by post-formulation procedure. These peptide-modified NPs varied in diameter and dispersity depending on the considered peptides and/or (co)polymers but kept their spherical shape. The peptide-functionalized NPs were more efficiently internalized by HepaRG hepatoma cells than native and maleimide-NPs with various levels relying on the peptide’s nature and the presence of PEG. We also observed important differences in internalization of NPs functionalized by the maleimide-thiol-peptide reaction compared to that of NPs prepared from peptide-functionalized PMLABe73 derivatives.
Electrospinning-Generated Nanofiber Scaffolds Suitable for Integration of Primary Human Circulating Endothelial Progenitor Cells
Authors: Jiménez-Beltrán Miguel A. , Gómez-Calderón Alan J. , Quintanar-Zúñiga Rafael E. , Santillán-Cortez Daniel , Téllez-González Mario A. , Suárez-Cuenca Juan A. , García Silvia , Mondragón-Terán Paul
Published: June 2022

The extracellular matrix is fundamental in order to maintain normal function in many organs such as the blood vessels, heart, liver, or bones. When organs fail or experience injury, tissue engineering and regenerative medicine elicit the production of constructs resembling the native extracellular matrix, supporting organ restoration and function. In this regard, is it possible to optimize structural characteristics of nanofiber scaffolds obtained by the electrospinning technique? This study aimed to produce partially degraded collagen (gelatin) nanofiber scaffolds, using the electrospinning technique, with optimized parameters rendering different morphological characteristics of nanofibers, as well as assessing whether the resulting scaffolds are suitable to integrate primary human endothelial progenitor cells, obtained from peripheral blood with further in vitro cell expansion. After different assay conditions, the best nanofiber morphology was obtained with the following electrospinning parameters: 15 kV, 0.06 mL/h, 1000 rpm and 12 cm needle-to-collector distance, yielding an average nanofiber thickness of 333 ± 130 nm. Nanofiber scaffolds rendered through such electrospinning conditions were suitable for the integration and proliferation of human endothelial progenitor cells.
Preparation of Poly(vinyl Alcohol) Microparticles for Freeze Protection of Sensitive Fruit Crops
Authors: Sabando Constanza , Ide Walther , Rodríguez-Llamazares Saddys , Bastías Richard M. , Valenzuela Miguel , Rojas Claudio , Castaño Johanna , Pettinelli Natalia , Bouza Rebeca , Müller Niels
Published: June 2022

Poly(vinyl alcohol) (PVA) displays ice recrystallization inhibition (IRI) properties as many antifreeze proteins found in cold tolerant organisms. The molecular architecture and composition (molecular weight and distribution of pendant OH and acetate groups) have been studied to improve the antifreezing properties of PVA, suggesting that the molecular architecture of PVA plays an important role in IRI activity. The present work deals with the preparation of PVA microparticles using an alkaline treatment. The effect of PVA molecular weight on the morphology and antifreezeing properties of PVA microparticles was investigated. The antifreezeing property of PVA microparticles on the susceptibility of flower bud tissues to freeze damage was also evaluated. The alkaline treatment of an aqueous PVA solution produced stable polymer chain aggregates with spherical shapes. The average size of the PVA microparticles increased significantly with the increasing molecular weight of the PVA macromolecule precursor. The PVA microparticles inhibited the growth of ice crystals and blocked ice growth at concentrations as low as 0.01 % w/v. The effect of impeding ice crystal growth by preventing the joining of adjacent ice crystals is attributed to the larger size of the PVA particles adsorbed on the ice surface compared to the aggregated PVA macromolecules in saline solution. The thermal hysteresis activity of PVA macromolecules and microparticles was not detected by differential scanning calorimetry analysis. The PVA microparticles reduced the incidence of freeze injuries in flower bud tissues by 55% and their application, considering the low toxicity of PVA, has a high potential for freeze protection in fruit crops.
DC Surface Flashover Characteristics of Polyimide Containing Polyhedral Oligomeric Silsesquioxane (POSS) in the Main Chains under Vacuum
Authors: Wang Jian , Xiao Ruofan , Liu Renying , Ping An , Wang Zhe , Liu Jikui , Zhang Shumin , Liu Yanmin
Published: June 2022

Polyimide, which is widely used to insulate power equipment operating in a vacuum environment, is prone to insulation failure due to surface flashover. Using POSS to modify it is an effective solution. This paper focuses on the study of DC surface flashover characteristics in vacuum of POSS/polyimide composite film, by introducing 1%, 3%, 5% equivalent mole content of POSS into polyimide, and conducting a surface flashover characteristics test in vacuum together with pure polyimide. The physical and chemical properties of the composite films were tested utilizing Fourier transform infrared spectroscopy and ultraviolet–visible spectroscopy. Combined with resistivity, SEM, and other test techniques, the influence mechanism of POSS molecular modification on DC surface flashover characteristics of polyimide films in vacuum was initially revealed. The results showed that after the introduction of POSS, the overall functional group structure of polyimide remained unchanged, the intermolecular charge transfer complexation was inhibited, and the transmittance of the film increased. The thermal conductivity and thermogravimetric temperature of the film are improved to a certain extent, and the mechanical properties are slightly decreased. With the increase of the introduced POSS content, the dielectric strength of the composite film is also enhanced. The surface flashover voltage of the composite film with a POSS content of 5% is 17.5 kV in vacuum, which is 30.5% higher than that of the pure film. Further analysis shows that the introduction of POSS will reduce the resistivity of the composite film, accelerate the dissipation of surface charges, and increase the flashover voltage. In addition, POSS forms a uniformly distributed Si-O-Si cage-like structure through molecular modification. When the surface of the film is damaged, SiOx inorganic flocculent particles are generated, which can not only scatter electrons, but also shallow the depth of trap energy level and accelerate the dissipation rate of surface charge, thus increasing the flashover voltage.
Combined Strategy of Wound Healing Using Thermo-Sensitive PNIPAAm Hydrogel and CS/PVA Membranes: Development and In-Vivo Evaluation
Authors: Chu Yan , Chai Shuo , Li Fei , Han Cuiyan , Sui Xiaoyu , Liu Tingting
Published: June 2022

Past studies have shown that the hot spring effect can promote wound healing. Mild thermal stimulation and metal ions can promote angiogenesis. In this study, the hot spring effect was simulated by thermosensitive PNIPAAm hydrogel loaded with copper sulfide nanoparticles. Heat stimulation could be generated through near-infrared irradiation, and copper ions solution could be pulsed. On the other hand, the CS/PVA nanofiber membrane was attached to the bottom of the hydrogel to simulate the extracellular matrix structure, thus improving the wound healing ability. The CS/PVA nanofiber membrane was prepared by electrospinning, and the appropriate prescription and process parameters were determined. The nanofiber membrane has uniform pore size, good water absorption and permeability. The poor mechanical properties of PNIPAAm hydrogel were improved by adding inorganic clay. The temperature of the hydrogel loaded with CuS nanoparticles reached 40 °C under near-infrared light irradiation for 20 min, and the release rate of Cu2+ reached 26.89%. The wound-healing rate of the rats in the combined application group reached 79.17% at 13 days, demonstrating superior results over the other control groups. Histological analyses show improved inflammatory response at the healed wound area. These results indicate that this combined application approach represents a promising wound treatment strategy.
Towards an All-Solid-State Electrochromic Device: A Review of Solid-State Electrolytes and the Way Forward
Authors: Au Benedict Wen-Cheun , Chan Kah-Yoong
Published: June 2022

In order to curb high electricity usage, especially in commercial buildings, smart windows, also known as “switchable” or “smart” glasses, have attracted a significant amount of attention in an effort to achieve energy savings in eco-friendly buildings and transportation systems. Smart windows save energy by regulating the input of solar heat and light and hence cutting down air-conditioning expenses, while maintaining indoor comfort. This is achieved by electrochromism, which is defined as the reversible colour change in electrochromic (EC) materials from transparent to dark blue and vice versa under a small applied voltage. Recent state-of-the-art electrochromic devices (ECD) adopt liquid-based electrolytes as the main source of energy for basic operations. While this has resulted in much success in ECDs as reported in past studies, there remain several drawbacks to this aspect, such as liquid electrolyte leakage and evaporation, not to mention safety concerns related to the harmful nature of electrolyte materials. This paper aims to review the recent advances in various solid electrolytes that are potential solutions to the mentioned problems.
Boosting the Anticancer Activity of Sunitinib Malate in Breast Cancer through Lipid Polymer Hybrid Nanoparticles Approach
Authors: Ahmed Mohammed Muqtader , Anwer Md. Khalid , Fatima Farhat , Aldawsari Mohammed F. , Alalaiwe Ahmed , Alali Amer S. , Alharthi Abdulrahman I. , Kalam Mohd Abul
Published: June 2022

In the current study, lipid-polymer hybrid nanoparticles (LPHNPs) fabricated with lipoid-90H and chitosan, sunitinib malate (SM), an anticancer drug was loaded using lecithin as a stabilizer by employing emulsion solvent evaporation technique. Four formulations (SLPN1–SLPN4) were developed by varying the concentration of chitosan polymer. Based on particle characterization, SLPN4 was optimized with size (439 ± 5.8 nm), PDI (0.269), ZP (+34 ± 5.3 mV), and EE (83.03 ± 4.9%). Further, the optimized formulation was characterized by FTIR, DSC, XRD, SEM, and in vitro release studies. In-vitro release of the drug from SPN4 was found to be 84.11 ± 2.54% as compared with pure drug SM 24.13 ± 2.67%; in 48 h, release kinetics followed the Korsmeyer–Peppas model with Fickian release mechanism. The SLPN4 exhibited a potent cytotoxicity against MCF-7 breast cancer, as evident by caspase 3, 9, and p53 activities. According to the findings, SM-loaded LPHNPs might be a promising therapy option for breast cancer.
Alginate-Based Hydrogel as Delivery System for Therapeutic Bacterial RNase
Authors: Bogdanova Liliya R. , Zelenikhin Pavel V. , Makarova Anastasiya O. , Zueva Olga S. , Salnikov Vadim V. , Zuev Yuriy F. , Ilinskaya Olga N.
Published: June 2022

To deliver therapeutic proteins into a living body, it is important to maintain their target activity in the gastrointestinal tract after oral administration. Secreted ribonuclease from Bacillus pumilus (binase) has antitumor and antiviral activity, which makes it a promising therapeutic agent. This globular protein of small molecular weight (12.2 kDa) is considered as a potential agent that induces apoptosis of tumor cells expressing certain oncogenes, including colorectal and duodenum cancer. The most important problem of its usage is the preservation of its structure and target activity, which could be lost during oral administration. Here, we developed alginate microspheres reinforced with divalent cations and analyzed the enzyme release from them. Using methods of scanning electron microscopy, measurements of fluorescence, enzyme catalytic activity, and determination of viability of the duodenum adenocarcinoma tumor cell line, we characterized obtained microspheres and chose calcium as a biogenic ion-strengthening microsphere structure. Among such modified additivities as beta-casein, gelatin, and carbon nanotubes introduced into microspheres, only gelatin showed a pronounced increase in their stability and provided data on the prolonged action of enzyme release from microspheres into tumor cell culture medium during 48 h in an amount of about 70% of the loaded quantity.
Post-Consumer Textile Waste Classification through Near-Infrared Spectroscopy, Using an Advanced Deep Learning Approach
Authors: Riba Jordi-Roger , Cantero Rosa , Riba-Mosoll Pol , Puig Rita
Published: June 2022

The textile industry is generating great environmental concerns due to the exponential growth of textile products’ consumption (fast fashion) and production. The textile value chain today operates as a linear system (textile products are produced, used, and discarded), thus putting pressure on resources and creating negative environmental impacts. A new textile economy based on the principles of circular economy is needed for a more sustainable textile industry. To help meet this challenge, an efficient collection, classification, and recycling system needs to be implemented at the end-of-life stage of textile products, so as to obtain high-quality recycled materials able to be reused in high-value products. This paper contributes to the classification of post-consumer textile waste by proposing an automatic classification method able to be trained to separate higher-quality textile fiber flows. Our proposal is the use of near-infrared (NIR) spectroscopy combined with a mathematical treatment of the spectra by convolutional neural networks (CNNs) to classify and separate 100% pure samples and binary mixtures of the most common textile fibers. CNN is applied for the first time to the classification of textile samples. A total of 370 textile samples were studied—50% used for calibration and 50% for prediction purposes. The results obtained are very promising (100% correct classification for pure fibers and 90–100% for binary mixtures), showing that the proposed methodology is very powerful, able to be trained for the specific separation of flows, and compatible with the automation of the system at an industrial scale.
The Impact of Particulate Matters and Nanoparticles on Thermoplastic Polymer Coatings and Paint Layers
Authors: Héritier Pierre-Antoine
Published: June 2022

This article attempts to highlight a phenomenon that more or less permanently damages emulsion paint layers, the surfaces of which remain sufficiently permeable for dust particles to become permanently anchored there; when the particles are nanometric, this can cause a permanent change in appearance. Based on scientific documents, empirical observations, laboratory analyses, case studies, and reconstructions of characteristic pictorial layers, this paper aims to highlight the medium- and long-term risks that alter these surfaces, in order to realize strategies for better prevention. The physico-chemical nature of these vulnerable materials will be discussed first, followed by the dust’s involvement; finally, the topic will be illustrated through concrete examples, with photos taken using digital, 4 K optical, and Scanning Electron Microscope equipment (SEM), in order to show how the problem of dust particle accumulation impacts even the most contemporary works of art.
Everyone’s Accountable? Peer Sexual Abuse in Religious Schools, Digital Revelations, and Denominational Contests over Protection
Authors: Kasstan Ben
Published: June 2022

Since the emergence of the #MeToo and #ChurchToo movements, online tracts have been employed to publicly reveal experiences of sexual abuse and assault among women and men in religious institutions and to shame abusers, which tend to be examined as an issue of women’s rights or child protection from adult predators. Drawing on the use of digital reporting platforms to testify against peer offences within religious schools, this paper asks how do such testimonies reveal adolescent agency and provoke policy re/actions about the accountability of religious institutions? Digital revelations submitted anonymously to Everyone’s Invited are analysed alongside interviews conducted with educators, parents, and youths in Jewish schools in Britain. Findings indicate how adolescent digital revelations of peer sexual abuse call for accountability by implicating the faith schools in question, which in turn triggers pedagogical and policy debates from educators. Public responses reflect diverging denominational positions on how to balance the protection of young people and safeguard religious self-protectionism. The paper spotlights the agency of youth in shaming peer abusers as much as faith schools and structures of religious authority, and in turn, how online shaming reveals frictions over accountability.
Freedom of Conscience of Healthcare Professionals and Conscientious Objection in the European Court of Human Rights
Authors: Valero María José
Published: June 2022

The recent social and legal debate in several European countries on abortion, euthanasia, and assisted suicide has caused a strong resurgence of the concerns of healthcare personnel as to the real possibility of protecting their consciences in their professional sphere. Individual refusal for religious, moral, deontological, or ethical reasons to participate in activities that directly or indirectly could result in the termination of a human life constitutes the most extreme manifestation of the legal phenomenon of conscientious objection. Although the European Convention on Human Rights does not recognize a general right to conscientious objection, since Bayatyan v. Armenia, the case law of the European Court of Human Rights has identified a connection between conscience-related claims to compulsory military service and Article 9 of the Convention. However, to this date, this doctrine has not been applied to cases that affect health-sensitive areas like abortion and contraception. This article analyzes the activity of the European Court of Human Rights in relation to the right to freedom of conscience and to conscientious objection, particularly in healthcare, and offers several final observations projected to possible future conflicts.
Exploring Motivation and Engagement: Voices of Adolescent Non-Arab Muslim Learners of Arabic at Australian Islamic Schools
Authors: Selim Nadia , Abdalla Mohamad
Published: June 2022

This article explores the motivation and engagement of adolescent non-Arab Muslim learners of Arabic (a-MLA) enrolled at Australian Islamic Schools (AIS). To this end, the ‘L2 Motivational Self System’ was used as a theoretical lens. This research gives ‘voice’ to learners and is dialogic, ‘speaking with’ rather than ‘speaking for’ learners in Islamic schools. It also responds to calls for the ‘renewal’ of Islamic Schools in the Western context, including in Australia, through a focus on Arabic learning. A basic interpretive qualitative approach was used, and data were collected from 40 participants using semi-structured interviews. The interviews were supplemented by classroom observations. In keeping with the emphasis placed on learners’ voices, the data presented focus on the students’ own words and perspectives. The findings suggest the presence of predominantly religious orientations to learning Arabic, but that a subset of other orientations also exists. The findings also indicate that several contextual factors can lead to disengagement and that the L2 Motivational Self System might not fully explain the situation of these learners. Nonetheless, these findings can inform the practice of teachers engaged with a-MLA and provide grounds for further research.
Going to the Morgue with Andres Serrano: Provocation as Revelation
Authors: Sosler Alex
Published: June 2022

Originally displayed in Paula Cooper Gallery in New York City’s SoHo district, Andres Serrano’s The Morgue series continued the artist’s controversial and transgressive work. Set against a black backdrop in a mortuary, he photographed dead bodies in different stages of decomposition. In this article, I borrow from Charles Taylor’s cultural analysis of the secular and Flannery O’Connor’s literary theory of the revelatory power of the grotesque to discuss Serrano’s artistic choices. In essence, I argue that his work is not a desecration of humanity but a stark reminder of the sacralization of humanity. As such, Serrano’s work is not provocative for provocation’s sake, but a provocation to poke holes in a disenchanted age. Underneath Serrano’s images is the question: if this is a heap of flesh, why are you provoked? In a culture that avoids death at all costs, Serrano reminds the contemporary world of their mortality with an updated form of memento mori art.