bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring Pakistani Physicians' Knowledge and Practices Regarding High Alert Medications: Findings and Implications
Authors: Mustafa Zia Ul , Haroon Shahzaib , Aslam Naeem , Saeed Ahsan , Salman Muhammad , Hayat Khezar , Shehzadi Naureen , Hussain Khalid , Khan Amer Hayat
Published: March 2022

Introduction: While many low-middle income countries (LMICs), including Pakistan, try and ensure patient safety within available resources, there are considerable concerns with medication use. Unsafe and inappropriate medication use, especially high alert medications (HAMs), is one of the important factors compromising patient safety and quality of care. Besides economic loss, HAMs contribute to greater morbidity, hospitalization, and mortality. Physicians as key members of the provision of healthcare are expected to be well aware of the administration and regulations surrounding HAMs. However, the current status is unknown in Pakistan. Consequently, the objectives of this study were to evaluate the knowledge of Pakistani physicians about the administration, regulation, and practices related to HAMs. This builds on our recently published study with nurses.

Methods: An online cross-sectional study design was used, and data were gathered from the physicians throughout Pakistan using previously used self-administered questionnaires during a period of 5 months (January 1 to May 30, 2021). All data were entered and analyzed using SPSS 22 for Windows.

Results: Physicians (847) who provided consent were enrolled in the study. Most physicians (62.2%) were male, aged between 25 and 30 years (75.2%) and had 2- to 5-year work experience (50.9%). About 27% were working in the emergency departments. The median (IQR) knowledge score for HAMs administration and regulation was 5 (3) and 5 (2), respectively. About 46.4% of respondents were found to have moderate knowledge about HAMs; increasing age, work experience, and higher qualifications were significantly associated (p < 0.05) with better HAMs knowledge. Around 58% had good practices relating to HAMs during their routine work. Median practice scores increased significantly (p < 0.05) with age, work experience, and postgraduate qualification.

Conclusion: Most Pakistani physicians possess moderate knowledge about HAMs administration and regulations. However, their practices relating to the HAMs administration and regulations are typically sub-optimal. Consequently, HAMs awareness needs to be improved by including course content in the current curriculum, provision of hospital-based continuous training programs about patient safety and care, and establishment of multi-disciplinary health care teams, including board-certified pharmacists and specialized nurses, for the effective execution of medication use process in Pakistani hospitals in the future.
CD44/Folate Dual Targeting Receptor Reductive Response PLGA-Based Micelles for Cancer Therapy
Authors: Zhang Ru , Jiang Yunying , Hao Linkun , Yang Yang , Gao Ying , Zhang Ningning , Zhang Xuecheng , Song Yimin
Published: March 2022

In this study, a novel poly (lactic-co-glycolic acid) (PLGA)-based micelle was synthesized, which could improve the therapeutic effect of the antitumor drug doxorubicin hydrochloride (DOX) and reduce its toxic and side effects. The efficient delivery of DOX was achieved by active targeting mediated by double receptors and stimulating the reduction potential in tumor cells. FA-HA-SS-PLGA polymer was synthesized by amidation reaction, and then DOX-loaded micelles were prepared by dialysis method. The corresponding surface method was used to optimize the experimental design. DOX/FA-HA-SS-PLGA micelles with high drug loading rate and encapsulation efficiency were prepared. The results of hydrophilic experiment, critical micelle concentration determination, and hemolysis test all showed that DOX/FA-HA-SS-PLGA micelles had good physicochemical properties and biocompatibility. In addition, both in vitro reduction stimulus response experiment and in vitro release experiment showed that DOX/FA-HA-SS-PLGA micelles had reduction sensitivity. Molecular docking experiments showed that it can bind to the target protein. More importantly, in vitro cytology studies, human breast cancer cells (MCF-7), human non-small cell lung cancer cells (A549), and mouse colon cancer cells (CT26) were used to demonstrate that the dual receptor-mediated endocytosis pathway resulted in stronger cytotoxicity to tumor cells and more significant apoptosis. In and in vivo antitumor experiment, tumor-bearing nude mice were used to further confirm that the micelles with double targeting ligands had better antitumor effect and lower toxicity. These experimental results showed that DOX/FA-HA-SS-PLGA micelles have the potential to be used as chemotherapeutic drugs for precise tumor treatment.
Limits of Prediction for Machine Learning in Drug Discovery
Authors: von Korff Modest , Sander Thomas
Published: March 2022

In drug discovery, molecules are optimized towards desired properties. In this context, machine learning is used for extrapolation in drug discovery projects. The limits of extrapolation for regression models are known. However, a systematic analysis of the effectiveness of extrapolation in drug discovery has not yet been performed. In response, this study examined the capabilities of six machine learning algorithms to extrapolate from 243 datasets. The response values calculated from the molecules in the datasets were molecular weight, cLogP, and the number of sp3-atoms. Three experimental set ups were chosen for response values. Shuffled data were used for interpolation, whereas data for extrapolation were sorted from high to low values, and the reverse. Extrapolation with sorted data resulted in much larger prediction errors than extrapolation with shuffled data. Additionally, this study demonstrated that linear machine learning methods are preferable for extrapolation.
A Multitarget Therapeutic Peptide Derived From Cytokine Receptors Based on in Silico Analysis Alleviates Cytokine-Stimulated Inflammation
Authors: Chang Chun-Chun , Peng Shih-Yi , Tsao Hao-Hsiang , Huang Hsin-Ting , Lai Xing-Yan , Hsu Hao-Jen , Jiang Shinn-Jong
Published: March 2022

Septicemia is a severe inflammatory response caused by the invasion of foreign pathogens. Severe sepsis-induced shock and multiple organ failure are the two main causes of patient death. The overexpression of many proinflammatory cytokines, such as TNF-α, IL-1β, and IL-6, is closely related to severe sepsis. Although the treatment of sepsis has been subject to many major breakthroughs of late, the treatment of patients with septic shock is still accompanied by a high mortality rate. In our previous research, we used computer simulations to design the multifunctional peptide KCF18 that can bind to TNF-α, IL-1β, and IL-6 based on the binding regions of receptors and proinflammatory cytokines. In this study, proinflammatory cytokines were used to stimulate human monocytes to trigger an inflammatory response, and the anti-inflammatory ability of the multifunctional KCF18 peptide was further investigated. Cell experiments demonstrated that KCF18 significantly reduced the binding of proinflammatory cytokines to their cognate receptors and inhibited the mRNA and protein expressions of TNF-α, IL-1β, and IL-6. It could also reduce the expression of reactive oxygen species induced by cytokines in human monocytes. KCF18 could effectively decrease the p65 nucleus translocation induced by cytokines, and a mice endotoxemia experiment demonstrated that KCF18 could reduce the expression of IL-6 and the increase of white blood cells in the blood stimulated by lipopolysaccharides. According to our study of tissue sections, KCF18 alleviated liver inflammation. By reducing the release of cytokines in plasma and directly affecting vascular cells, KCF18 is believed to significantly reduce the risk of vascular inflammation.
Inhibition of Microbiota-dependent Trimethylamine N-Oxide Production Ameliorates High Salt Diet-Induced Sympathetic Excitation and Hypertension in Rats by Attenuating Central Neuroinflammation and Oxidative Stress
Authors: Liu Gang , Cheng Jiayin , Zhang Tianhao , Shao Yingxin , Chen Xiangxu , Han Lihong , Zhou Ru , Wu Bin
Published: March 2022

Excessive dietary salt intake induces neuroinflammation and oxidative stress in the brain, which lead to sympathetic excitation, contributing to hypertension. However, the underlying mechanisms remain elusive. Accumulating evidence reveals that trimethylamine-N-oxide (TMAO), a gut microbiota-derived metabolite, is implicated in the pathogenesis of multiple cardiovascular diseases. The present study sought to determine whether central TMAO is elevated and associated with neuroinflammation and oxidative stress in the brain after long-term high salt (HS) diet intake and, if so, whether inhibition of TMAO generation ameliorates HS-induced sympathetic excitation and hypertension. Sprague–Dawley rats were fed either a HS diet or a normal salt (NS) diet and simultaneously treated with vehicle (VEH) or 1.0% 3,3-Dimethyl-1-butanol (DMB, an inhibitor of trimethylamine formation) for 8 weeks. HS + VEH rats, compared with NS + VEH rats, had elevated TMAO in plasma and cerebrospinal fluid (CSF), increased blood pressure (BP), and increased sympathetic drive as indicated by the BP response to ganglionic blockade and plasma norepinephrine levels. HS-induced these changes were attenuated by DMB, which significantly reduced TMAO in plasma and CSF. Neuroinflammation as assessed by proinflammatory cytokine expression and NF-κB activity and microglial activity, and oxidative stress as measured by NAD(P)H oxidase subunit expression and NAD(P)H activity and reactive oxygen species (ROS) production in the hypothalamic paraventricular nucleus (PVN) were increased in HS + VEH rats but were decreased by DMB. DMB had no effects on above measured parameters in NS rats. The results suggest that long-term HS diet intake causes elevation in TMAO in the circulation and brain, which is associated with increased neuroinflammation and oxidative stress in the PVN, an important cardiovascular regulatory center. Inhibition of TMAO generation ameliorates HS-induced sympathetic excitation and hypertension by reducing neuroinflammation and oxidative stress in the PVN.
Temporal-Spatial Measurement of Surface Morphological Evolution Time in Nanosecond Laser Irradiation on the Copper Film
Authors: Liu Yuhan , Zhang Jianguo , Shangguan Shiyong , Qi Dongfeng , Sun Tengfei , Zheng Hongyu
Published: March 2022

In this research, the pump-probe imaging technology and the time-resolved transmittance measurements are used to investigate the transient time scale of the oxidation area, the dewetted melt pool, and the ablation-induced mass transport, and their dependence on the incident laser. Moreover, the evolution time of various morphologies have been studied through a certain delay time from 10 to 30 ns with different dynamic processes. Meanwhile, the underlying formation mechanism and the phase change pathway have been studied. In this regard, a nanosecond (ns) pulsed laser with 532 nm wavelength was used to fabricate nanostructures on the surface of the thin Copper (Cu) films. The observations confirmed that the oxidation process of Cu plays a significant role in the mechanism of laser fluence-dependent oxidation, melting, and ablation dynamics. The results revealed that the different characteristics of the Cu film are related to the incident laser pulse energy. Furthermore, the experimental evidence was in good agreement with the SEM images and the Raman spectra. It is found that the Cu2O surface-rim with a central hole could be fabricated with the laser fluences above the ablation threshold. Although, for the laser fluences below the ablation threshold, the Cu2O phase can be formed in the central region.
Defining Optimal Cut-Points for Cardiorespiratory Fitness Associated With Overweight/Obesity in Children: A School-Based Study
Authors: Kasović Mario , Štefan Lovro , Petrić Vilko , Štemberger Vesna , Blažević Iva
Published: March 2022

The main purpose of the study was to define optimal criterion-referenced cut-points for cardiorespiratory fitness (CRF) associated with overweight/obesity. In this cross-sectional study, participants were 1,612 children aged 7–14 years (mean age ± SD = 9.7 ± 2.4 years; 52.5% girls). CRF was assessed by the Maximal multistage 20-m shuttle run test, from which maximal oxygen uptake (VO2max) was estimated. Anthropometric indices included body-mass index (BMI), waist circumference (WC), and waist-to-height ratio (WHtR). Receiver operating characteristic (ROC) curves were performed to determine cut-off points. In boys, the optimal cut-off points of CRF in defining overweight/obesity for BMI, WC, and WHtR were 44.6, 46.4, and 46.9 mlO2/kg/min. The areas under the curves (AUC) were 0.83 (95% CI 0.78–0.88, p < 0.001), 0.77 (95% CI 0.71–0.83, p < 0.001), and 0.90 (95% CI 0.86–0.93, p < 0.001). In girls, the optimal cut-off points were 41.0, 40.8, and 40.7 mlO2/kg/min for BMI, WC, and WHtR, with the AUCs of 0.86 (95% CI 0.82–0.90, p < 0.001), 0.83 (95% CI 0.79–0.88), and 0.88 (95% CI 0.84–0.93, p < 0.001). In conclusion, our newly developed cut-off points for CRF assessed by the Maximal multistage 20-m shuttle run test may adequately detect primary school-aged boys and girls with general and abdominal obesity.
The Acute Physiological and Perceptual Responses Between Bodyweight and Treadmill Running High-Intensity Interval Exercises
Authors: Bellissimo Gabriella F. , Ducharme Jeremy , Mang Zachary , Millender Desmond , Smith Jessica , Stork Matthew J. , Little Johnathan P. , Deyhle Michael R. , Gibson Ann L. , de Castro Magalhaes Flavio , Amorim Fabiano
Published: March 2022

Objective

The purpose of this study was to compare the acute physiological, perceptual, and enjoyment responses between bodyweight high-intensity interval exercise (BW-HIIE) and treadmill running high-intensity interval exercise HIIE (RUN-HIIE).

Methods

Twelve adults [age: 29.5 ± 5.3 years; weight: 70.9 ± 15.0 kg; height: 167.9 ± 8.9 cm; peak oxygen consumption (VO2 peak): 48.7 ± 6.5 ml min−1·kg−1] performed both RUN-HIIE and BW-HIIE. RUN-HIIE consisted of two sets of 5, 60-s (s) run intervals at 100% of the speed achieved during VO2 peak testing followed by 60s of walking at 4.02 km/h. BW-HIIE consisted of two sets of 5, 60s ‘all-out’ effort calisthenic exercises followed by 60s of marching in place at 100 steps per minute. Oxygen consumption (VO2), blood lactate (Blac), heart rate (HR), and rating of perceived exertion (RPE) were measured during exercise. Physical activity enjoyment (PACES) was assessed post-exercise. Creatine Kinase (CK) was measured before exercise and 48-h post-exercise. Muscle soreness was assessed before exercise, post-exercise, and 48-h post-exercise.

Results

Oxygen consumption relative to VO2 peak was higher (p < 0.001) during RUN-HIIE (88 ± 3%) compared to BW-HIIE (77 ± 4%). HR relative to HRpeak was higher (p = 0.002) for RUN-HIIE (93 ± 1%) compared to BW-HIIE (88 ± 2%). Blac was higher (p < 0.001) after BW-HIIE (11.2 ± 3.2 mmol/l) compared to RUN-HIIE (6.9 ± 2.0 mmol/l). Average RPE achieved was higher (p = 0.003) during BW-HIIE (16 ± 2) than RUN-HIIE (14 ± 2). PACES was similar for RUN-HIIE and BW-HIIE (p > 0.05). No differences (p > 0.05) in CK were observed between RUN-HIIE and BW-HIIE.

Conclusion

Our results indicate ‘all-out’ calisthenic exercise can elicit vigorous cardiorespiratory, Blac, and RPE responses. Implementing this style of exercise into training requires minimal space, no equipment, and may elicit cardiometabolic adaptations seen with traditional forms of high-intensity exercise.
Light Color and the Commercial Broiler: Effect on Ocular Health and Visual Acuity
Authors: Remonato Franco Bruna , Leis Marina L. , Wong Melody , Shynkaruk Tory , Crowe Trever , Fancher Bryan , French Nick , Gillingham Scot , Schwean-Lardner Karen
Published: March 2022

Light is a critical management factor for broiler production, and the wavelength spectrum, one of its components, can affect bird physiology, behavior and production. Among all the senses, sight is important to birds, and their visual system possess several adaptations that allow them to perceive light differently from humans. Therefore, it is critical to consider whether the exposure to monochromatic light colors influences broiler visual ability, which could affect behavioral expression. The present study examined the effects of various light colors on the visual systems of broiler chickens. Ross 708 males were raised from 0 to 35 days under three wavelength programs [blue (dominant wavelengths near 455 nm), green (dominant wavelengths near 510 nm) or white]. Broilers were given a complete ophthalmic examination, including chromatic pupillary light reflex testing, rebound tonometry, anterior segment biomicroscopy and indirect ophthalmoscopy (n= 36, day 21). To assess ocular anatomy, broilers were euthanized, eyes were weighed, and dimensions were taken (n= 108, day 16 and day 24). An autorefractor was used to assess the refractive index and the corneal curvature (n= 18, day 26). To evaluate spatial vision, broilers underwent a grating acuity test at one of three distances–50, 75, or 100 cm (n= 24, day 29). Data were analyzed as a one-way ANOVA using the MIXED procedure or Proc Par1way for non-normally distributed data. Significant differences were observed for refractive index and spatial vision. Birds raised under blue light were slightly more hyperopic, or far-sighted, than birds raised under white light (P= 0.01). As for spatial vision, birds raised under blue light took less time to approach the stimulus at distances of 50 cm (P= 0.03) and 75 cm (P= 0.0006) and had a higher success rate (choosing the right feeder,P= 0.03) at 100 cm than birds raised under white light. Improvements in spatial vision for birds exposed to blue light can partially explain the behavioral differences resulting from rearing broilers under different wavelengths.
Future Time Orientation and Learning Engagement Through the Lens of Self-Determination Theory for Freshman: Evidence From Cross-Lagged Analysis
Authors: Peng Michael Yao-Ping , Zhang Zizai
Published: March 2022

View of future time orientation is a cognitive construct about future time. This view has its unique work of motivation and effect on academic performance. Previous studies have only explored the influence that future time orientation brings to the learning process at a single time, and most of them focus on cross-sectional studies. To further explore the cross-lagged relationship for freshmen between future time orientation and learning engagement during different periods, AMOS 23.0 was performed for cross-lagged analysis in this study to explore the influence and effect among variables of different periods. This research was based on the theory of self-determination to discover the relationship between future time and learning involvement for freshmen in enrollment and the first summer vacation. In this research, there were 1,000 valid samples in the first stage and 840 valid samples in the second stage for the conduction of descriptive statistics, pair t-test, and cross-lagged analysis. The results show that: (1) for learning engagement, freshmen at the end of the first year have a higher average score than at the beginning of the first year. (2) View of students of future time orientation can affect their learning engagement of the future through self-determination of students. At last, we provide some suggestions as references for institutional research and future research.
Examining Multiteam Systems Across Context and Type: A Historiometric Analysis of Failed MTS Performance
Authors: Campbell Lauren N. P. , Torres Elisa M. , Zaccaro Stephen J. , Zhou Steven , Hedrick Katelyn N. , Wallace David M. , Luning Celeste Raver , Zakzewski Joanna E.
Published: March 2022

Multiteam systems (MTSs) are complex organizational forms comprising interdependent teams that work towards their own proximal goals within and across teams to also accomplish a shared superordinate goal. MTSs operate within high-stakes, dangerous contexts with high consequences for suboptimal performance. We answer calls for nuanced exploration and cross-context comparison of MTSs “in the wild” by leveraging the MTS action sub-phase behavioral taxonomy to determine where and how MTS failures occur. To our knowledge, this is the first study to also examine how key MTS attributes (boundary status, goal type) influence MTS processes and performance. We conducted historiometric analysis on 40 cases of failed MTS performance across various contexts (e.g., emergency response, commercial transportation, military, and business) to uncover patterns of within- and between-team behaviors of failing MTSs, resulting in four themes. First, component teams of failing MTSs over-engaged in within-team alignment behaviors (vs. between-team behaviors) by enacting acting, monitoring, and recalibrating behaviors more often within than between teams. Second, failing MTSs over-focused on acting behaviors (vs. monitoring or recalibrating) and tended to not fully enact the action sub-phase cycle. Third and fourth, boundary status and goal type exacerbated these behavioral patterns, as external and physical MTSs were less likely to enact sufficient between-team behaviors or fully enact the action sub-phase cycle compared to internal and intellectual MTSs. We propose entrainment as a mechanism for facilitating MTS performance wherein specific, cyclical behavioral patterns enacted by teams align to facilitate goal achievementviathree multilevel behavioral cycles (i.e., acting-focused, alignment-focused, and adjustment-focused). We argue that the degree to which these cycles are aligned both between teams and with the overarching MTS goal determines whether and how an MTS fails. Our findings add nuance beyond single-context MTS studies by showing that the identified behavioral patterns hold both across contexts and almost all types of MTS action-phase behaviors. We show that these patterns vary by MTS boundary status and goal type. Our findings inform MTS training best practices, which should be structured to integrate all component teams and tailored to both MTS attributes (i.e., boundary status, goal type) and situation type (e.g., contingency planning).
Role of Program Curriculum in Building Social Skills and Sports Coaching in Academic and Career Development Under Sports Humanities and Sociology
Authors: Jiang Zhenglu , Yin Jiesen
Published: March 2022

This study focused on the role of program curriculum in building social skills and sports coaching in academic and career development in terms of sports humanities and sociology. Social skills coaching and sports coaching for the students are two significant factors that need to be considered by the universities around the globe to improve the organizational climate, which ultimately lead to better student’s career and academic development. This study utilized data from 308 members of the sports federation enrolled in different programs in the universities in China through a questionnaire for investigating such influencing factors. The data were analyzed using the structural equation modeling (SEM) technique using SmartPLS. This study found a substantial relationship of social skills with academic development and organizational climate. The relationship of sports coaching with organizational climate and career development is also discussed in this study. Furthermore, the organizational climate acts as a partial mediator between sports coaching and career development. However, this study revealed that organizational climate had an insignificant relationship with academic growth and organizational climate did not mediate between social skills coaching and educational development among students. While hiring the sports personnel, universities must consider the coach’s competency using effective methods such as physical tests, preassessments, and psychological tests. Second, the universities in China have to develop a culture of social skills and sports to improve academic and career development.
Automated Koos Classification of Vestibular Schwannoma
Authors: Kujawa Aaron , Dorent Reuben , Connor Steve , Oviedova Anna , Okasha Mohamed , Grishchuk Diana , Ourselin Sebastien , Paddick Ian , Kitchen Neil , Vercauteren Tom , Shapey Jonathan
Published: March 2022

Objective

The Koos grading scale is a frequently used classification system for vestibular schwannoma (VS) that accounts for extrameatal tumor dimension and compression of the brain stem. We propose an artificial intelligence (AI) pipeline to fully automate the segmentation and Koos classification of VS from MRI to improve clinical workflow and facilitate patient management.

Methods

We propose a method for Koos classification that does not only rely on available images but also on automatically generated segmentations. Artificial neural networks were trained and tested based on manual tumor segmentations and ground truth Koos grades of contrast-enhanced T1-weighted (ceT1) and high-resolution T2-weighted (hrT2) MR images from subjects with a single sporadic VS, acquired on a single scanner and with a standardized protocol. The first stage of the pipeline comprises a convolutional neural network (CNN) which can segment the VS and 7 adjacent structures. For the second stage, we propose two complementary approaches that are combined in an ensemble. The first approach applies a second CNN to the segmentation output to predict the Koos grade, the other approach extracts handcrafted features which are passed to a Random Forest classifier. The pipeline results were compared to those achieved by two neurosurgeons.

Results

Eligible patients (n = 308) were pseudo-randomly split into 5 groups to evaluate the model performance with 5-fold cross-validation. The weighted macro-averaged mean absolute error (MA-MAE), weighted macro-averaged F1 score (F1), and accuracy score of the ensemble model were assessed on the testing sets as follows: MA-MAE = 0.11 ± 0.05, F1 = 89.3 ± 3.0%, accuracy = 89.3 ± 2.9%, which was comparable to the average performance of two neurosurgeons: MA-MAE = 0.11 ± 0.08, F1 = 89.1 ± 5.2, accuracy = 88.6 ± 5.8%. Inter-rater reliability was assessed by calculating Fleiss' generalized kappa (k = 0.68) based on all 308 cases, and intra-rater reliabilities of annotator 1 (k = 0.95) and annotator 2 (k = 0.82) were calculated according to the weighted kappa metric with quadratic (Fleiss-Cohen) weights based on 15 randomly selected cases.

Conclusions

We developed the first AI framework to automatically classify VS according to the Koos scale. The excellent results show that the accuracy of the framework is comparable to that of neurosurgeons and may therefore facilitate management of patients with VS. The models, code, and ground truth Koos grades for a subset of publicly available images (n = 188) will be released upon publication.
Rehabilitation Research in Denmark Between 2001 and 2020: A Scoping Review
Authors: Momsen Anne-Mette Hedeager , Fox Jasmine Charlotte , Nielsen Claus Vinther , Thuesen Jette , Maribo Thomas
Published: March 2022

The demand for rehabilitation has increased, and evidence is rapidly growing; however, a rehabilitative health strategy receives less attention than treatment. Knowledge of what is being researched, who are the target groups and who contributes to rehabilitation research is deficient. We did not find any reviews mapping rehabilitation research regarding the research questions. The objective was to identify and synthesize existing scientific evidence on rehabilitation research published by Danish institutions between 2001 and 2021. The research questions to be explored were: Among which study groups has rehabilitation research been published?. Which types of studies on rehabilitation have been published?. Which institutions have been involved in rehabilitation research?

Methods

The process was guided according to the Joanna Briggs Institute's (JBI's) scoping review methodology. Four databases were searched. All types of peer-reviewed studies on any target group and rehabilitation setting, with any affiliation to a Danish institution, were eligible to be included. Studies referring to population and the type of design were categorized. Institutions were counted as Danish first authorship.

Results

The search revealed 3,100 studies, and following screening 1,779 were included. A total of 24 broad study groups were identified, mostly diagnosis-based health conditions. Musculoskeletal, cancer, and cardiac had 342, 228, and 174 studies, respectively. A total of 1,545 had a Danish first authorship, most of the Danish publications came from hospitals (56.6%) and universities (28.4%). The publication trend showed an almost linear development, with a 10–15% increase during the period.

Conclusion

Following screening 1,779 studies were included involving 24 broad study groups. Most categories were diagnosis-based; musculoskeletal, cancer, and cardiac health conditions encompassed most studies. All study designs were represented, and 1/10 were secondary studies. The majority (87%) of studies had a Danish first authorship. The majority of first affiliations were among hospitals followed by universities. A few municipalities were presented although they are yet to have research responsibility. Publication trends showed an increase primarily from 2013.

Systematic Review Registration

https://osf.io/, identifier [10.17605/OSF.IO/2AENX].
Moving Beyond Business as Usual Toward Regenerative Business Practice in Small and Medium-Sized Enterprises
Authors: Caldera Savindi , Hayes Samantha , Dawes Les , Desha Cheryl
Published: March 2022

Environmental degradation is a complex global challenge requiring the urgent attention of small and medium-sized enterprises (SMEs), which are collectively responsible for a large proportion of global pollution. For those SMEs who are still thinking about sustainability at the level of the organization and reducing its environmental damage, there must be an immediate shift in SME strategy and operations to consider planetary systems and practices that can regenerate ecosystems critical for the business's success. Responding to this urgent need, the authors were keen to identify how SMEs could move from “doing less bad” to “doing more good,” as a critically needed shift toward “regenerative business practice.” Using two case studies of Australian manufacturing sector SMEs already self-identified as regenerative business practices, their transition pathways and current operations were explored for insights and lesson learning that could be used to empower other SMEs. Collected interview data revealed three themes of priority during the two SMEs' journeys: (1) Organization and Nature conviviality; (2) Organizational freedom to innovate; and (3) Organizational innovative outlook. The SMEs' experiences were also explored in relation to an “Action Framework for Regenerative Business” developed by the authors. The framework draws on Stewardship Theory together with a set of “Principles and Strategies of Regenerative Business” for SMEs to consider their current operations and identify opportunities for their next steps accordingly. Such directed actions are imperative to move away from just “reducing pollution” to “restoring planetary systems,” demonstrating truly responsible consumption and production. Within the framework the authors add “advocate” to the existing stewardship roles of “doers,” “donors” and “practitioners,” which acknowledges the importance of this role in enabling SMEs to shift practices; in this case to regenerative business practice.
Do Different Hurdle Heights Alter Important Spatiotemporal Variables in Hurdle Clearance?
Authors: Ozaki Yusuke , Ueda Takeshi
Published: March 2022

The aim of this study was to determine whether important spatiotemporal variables in hurdle clearance change with different hurdle heights. Twelve male hurdlers (mean height, 1.75 ± 0.04 m) cleared hurdles set at different heights [10% higher (High) and 10% lower (Low) than the center of mass (CM)]; images were captured by six high-speed cameras, and each spatiotemporal variable was calculated. Thereafter, the difference in each spatiotemporal variable between High and Low and the relationship between the mean horizontal velocity from takeoff to landing [Hurdle clearance velocity (HC-v)] and each spatiotemporal variable were examined. Our findings indicated that values for flight time, flight time from hurdle to landing (2nd flight time), clearance time, release height on takeoff, peak height of the CM, and the difference in landing distance were greater in the High condition than in the Low condition. Moreover, a low rate of deceleration on takeoff and short 2nd flight time, clearance distance, and takeoff distance were more strongly related to HC-v in the condition High, whereas a low rate of deceleration on landing, short flight time from takeoff to hurdle (1st flight time), high release height on landing, and touchdown height on landing were more strongly related to HC-v in the Low condition. Therefore, coaches should consider these changes in spatiotemporal variables when changing hurdle heights based on age group or event. It should also be noted that, even when the hurdle heights are the same, the spatiotemporal variables that should be considered may differ depending on the height of the hurdler.
An Innovative Prone Position Using a Body-Shape Plaster Bed and Skull Traction for Posterior Cervical Spine Fracture Surgeries
Authors: Ding Zhiyu , Ren Yijun , Cao Hongqing , Li Yuezhan , Chen Shijie , Miao Jinglei , Li Jinsong
Published: March 2022

Background

An innovative prone cervical spine surgical position using a body-shape plaster bed with skull traction (BSPST) was compared with the traditional prone surgical position with horseshoe headrests.

Methods

A total of 47 patients, undergoing posterior cervical spine surgery for cervical spine fracture, were retrospectively classified into two groups, the BSPST group (n = 24) and the traditional group (n = 23), and underwent a posterior instrumented fusion with or without decompression. Multiple indicators were used to evaluate the advantages of the BSPST compared with the traditional position.

Results

All the operations went smoothly. The mean recovery rate was 56.30% in the BSPST group and 48.55% in the traditional group (p = 0.454), with no significant difference. The intraoperative blood loss (177.5 ml vs. 439.1 ml, p = 0.003) and the total incidence of complications (8.3 vs. 47.8%, p = 0.004) were significantly less in the BSPST group than in the traditional group. In addition, the BSPST position provided a greater comfort level for the operators and allowed convenient intraoperative radiography.

Conclusions

This is the first study to describe a combined body-shape plaster bed and skull traction as an innovative cervical spine-prone surgical position that is simple, safe, and stable, intraoperative traction direction adjustable, reproducible, and economical for posterior cervical spine fracture surgery, and potentially other cervical and upper dorsal spine surgeries in the prone position. Additionally, this position provides the surgeons with a comfortable surgical field and can be easily achieved in most orthopedic operation rooms.
Genome Wide Scan to Identify Potential Genomic Regions Associated With Milk Protein and Minerals in Vrindavani Cattle
Authors: Singh Akansha , Kumar Amit , Gondro Cedric , Pandey A. K. , Dutt Triveni , Mishra B. P.
Published: March 2022

In this study, genome-wide association study (GWAS) was conducted for identifying significantly associated genomic regions/SNPs with milk protein and minerals in the 96 taurine-indicine crossbred (Vrindavani) cows using 50K SNP Chip. After quality control, a total of 41,427 SNPs were retained and were further analyzed using a single-SNP additive linear model. Lactation stage, parity, test day milk yield and proportion of exotic inheritance were included as fixed effects in GWAS model. Across all traits, 13 genome-wide significant (p < 1.20 x 10−06) and 49 suggestive significant (p < 2.41 x 10−05) SNPs were identified which were located on 18 different autosomes. The strongest association for protein percentage, calcium (Ca), phosphorus (P), copper (Cu), zinc (Zn), and iron (Fe) were found on BTA 18, 7, 2, 3, 14, and 2, respectively. No significant SNP was detected for manganese (Mn). Several significant SNPs identified were within or close proximity to CDH13, BHLHE40, EDIL3, HAPLN1, INHBB, USP24, ZFAT, and IKZF2 gene, respectively. Enrichment analysis of the identified candidate genes elucidated biological processes, cellular components, and molecular functions involved in metal ion binding, ion transportation, transmembrane protein, and signaling pathways. This study provided a groundwork to characterize the molecular mechanism for the phenotypic variation in milk protein percentage and minerals in crossbred cattle. Further work is required on a larger sample size with fine mapping of identified QTL to validate potential candidate regions.
Transcriptome Comparison of Chorion-Attached and Non-chorion-attached Endometrium in Mid-gestation of Rabbit
Authors: Mei Xiuli , Xu Ling , Ren Yan , Yu Minjie , Kuang Liangde , Li Congyan , Zhang Yan , Lu Chuanzhi , Wang Zhicheng , Guo Zhiqiang , Xie Xiaohong , Huang Dengping , Zhang Ming
Published: March 2022

Background

The chorion from the placenta is directly attached to the endometrium (CA) after embryo implantation while some parts of the endometrium are not chorion-attached (NCA). The differences in gene expression between the CA and NCA endometrium mid-gestation are unknown. Our objective was to compare the gene expression profiles of the CA and NCA endometrium of rabbit, to identify the differentially expressed genes (DEGs), and correlate the differences with the physiological state of the endometrium at mid-gestation of rabbit.

Methods

We used transcriptome sequencing to reveal the differences in gene expression between CA and NCA endometrium (n = 3), and then determined the concentration of inflammatory cytokines in CA and NCA tissue and serum by ELISA.

Results

Six Hundred and Forty-Six DEGs were identified between the CA and NCA endometrium [p < 0.05, |log2 (fold change) |≥ 2], The expression levels of 590 DEGs were higher in the NCA endometrium than in the CA endometrium, while the expression level of only 56 DEGs were higher in CA than in NCA. The DEGs were enriched in gene ontology (GO) terms and pathways related to immune regulation and cellular adhesions. Six hub-genes related to inflammatory mediator regulation of transient receptor potential (TRP) channels and chemokine signaling pathways had a lower expression level in the CA endometrium compared to the NCA endometrium, and the expression levels of genes related to focal adhesion and extracellular matrix (ECM)-receptors were significantly higher in NCA endometrium than in CA endometrium. The level of pro-inflammatory cytokines accumulated in the CA endometrium, and high abundance of integrin-β and THBS1 were localized in the luminal epithelium of the NCA endometrium, but not in the CA endometrium.

Conclusions

Our study reveals differences in gene expression between the CA and NCA endometrium at mid-gestation of rabbit, and suggests implications for endometrial physiological function. The CA endometrium showed relative low-level gene expression compared to the NCA endometrium, while the NCA endometrium performed physiological functions related to focal adhesion and ECM-receptor interaction.
Abstract Reservoir Computing
Authors: Senn Christoph Walter , Kumazawa Itsuo
Published: March 2022

Noise of any kind can be an issue when translating results from simulations to the real world. We suddenly have to deal with building tolerances, faulty sensors, or just noisy sensor readings. This is especially evident in systems with many free parameters, such as the ones used in physical reservoir computing. By abstracting away these kinds of noise sources using intervals, we derive a regularized training regime for reservoir computing using sets of possible reservoir states. Numerical simulations are used to show the effectiveness of our approach against different sources of errors that can appear in real-world scenarios and compare them with standard approaches. Our results support the application of interval arithmetics to improve the robustness of mass-spring networks trained in simulations.
Calcium Channels in the Heart: Disease States and Drugs
Authors: Shah Kajol , Seeley Sarah , Schulz Castin , Fisher Jacqueline , Gururaja Rao Shubha
Published: March 2022

Calcium ions are the major signaling ions in the cells. They regulate muscle contraction, neurotransmitter secretion, cell growth and migration, and the activity of several proteins including enzymes and ion channels and transporters. They participate in various signal transduction pathways, thereby regulating major physiological functions. Calcium ion entry into the cells is regulated by specific calcium channels and transporters. There are mainly six types of calcium channels, of which only two are prominent in the heart. In cardiac tissues, the two types of calcium channels are the L type and the T type. L-type channels are found in all cardiac cells and T-type are expressed in Purkinje cells, pacemaker and atrial cells. Both these types of channels contribute to atrioventricular conduction as well as pacemaker activity. Given the crucial role of calcium channels in the cardiac conduction system, mutations and dysfunctions of these channels are known to cause several diseases and disorders. Drugs targeting calcium channels hence are used in a wide variety of cardiac disorders including but not limited to hypertension, angina, and arrhythmias. This review summarizes the type of cardiac calcium channels, their function, and disorders caused by their mutations and dysfunctions. Finally, this review also focuses on the types of calcium channel blockers and their use in a variety of cardiac disorders.
Emerging SARS-CoV-2 Variants, Inequitable Vaccine Distribution, and Implications for COVID-19 Control in Sub-Saharan Africa
Authors: Murewanhema Grant , Dzinamarira Tafadzwa , Chingombe Innocent , Mapingure Munyaradzi Paul , Mukwenha Solomon , Chitungo Itai , Herrera Helena , Madziva Roda , Ngwenya Solwayo , Musuka Godfrey
Published: March 2022

Since the onset of the COVID-19 pandemic, four SARS-CoV-2 variants of concern have emerged, which have shifted the clinical and epidemiological characteristics of the disease. Of concern is the impact of the emerging variants on COVID-19 vaccination programmes, with vaccination perceived as a key global pandemic control strategy. Variants of concern can reduce the effectiveness of the currently available vaccines, shift herd immunity thresholds, and promote wider vaccine inequities as richer countries hoard vaccines for booster shots for their populations without accounting for the needs of the underdeveloped countries of sub-Saharan Africa. Currently, Africa lags far behind the rest of the world, with most sub-Saharan Africa countries still to reach 50% vaccination of their eligible populations against global herd immunity thresholds of 70–90%. As long as the vaccination gap between sub-Saharan Africa and the rest of the world persists, SARS-CoV-2 will most likely persist as a significant global health threat, with continued emergence of variants of concern. Therefore, strategies to ensure wider reach of different types of vaccines on the African continent are urgently required alongside fighting vaccine hesitancy and logistical barriers to access for the marginalized populations. Sub-Saharan Africa must look for opportunities to manufacture vaccines on the continent and enhance genomic sequencing capacity as key pandemic-control strategies.
T-cell Receptor Is a Threshold Detector: Sub- and Supra-Threshold Stochastic Resonance in TCR-MHC Clusters on the Cell Surface
Authors: Bene László , Bagdány Miklós , Damjanovich László
Published: March 2022

Stochastic resonance in clusters of major histocompatibility molecules is extended by a more detailed description of adaptive thresholding and by applying the notion of suprathreshold stochastic resonance as a stochastically quantizing encoder of transmembrane signaling downstream of major histocompatibility molecules and T-cell receptors on the side of presenting and recognizing cells, respectively. The adaptive nature of thresholding is partly explained by a mirroring of the noncognate–cognate dichotomy shown by the T-cell receptor structure and the kinetic-segregation model of the onset of T-cell receptor triggering. Membrane clusters of major histocompatibility molecules and T-cell receptors on their host cells are envisioned as places of the temporal encoding of downstream signals via the suprathreshold stochastic resonance process. The ways of optimization of molecular prostheses, such as chimeric antigen receptors against cancer in transmembrane signaling, are suggested in the framework of suprathreshold stochastic resonance. The analogy between Förster resonance energy transfer and suprathreshold stochastic resonance for information transfer is also discussed. The overlap integral for energy transfer parallels the mutual information transferred by suprathreshold stochastic resonance.
Variable Valve Strategy Evaluation for Low-Load Operation in a Heavy-Duty Gasoline Compression Ignition Engine
Authors: Kumar Praveen , Zhang Yu
Published: March 2022

By harnessing gasoline’s low reactivity for partially premixed combustion promotion, gasoline compression ignition (GCI) combustion shows the potential to produce markedly improved NOx-soot trade-off with high fuel efficiency compared to conventional diesel combustion. However, at low-load conditions, gasoline’s low reactivity poses challenges to attaining robust combustion with low unburned hydrocarbons (UHC) and carbon monoxide (CO) emissions. Increasing the in-cylinder charge temperature by using variable valve actuation (VVA) can be an effective means to address these challenges. In this numerical investigation, VVA strategies, including (1) early exhaust valve opening (EEVO), (2) positive valve overlap (PVO), and (3) exhaust rebreathe (ExReb), were investigated at 1375 RPM and 2 bar brake mean effective pressure in a heavy-duty GCI engine using a market-based gasoline with a research octane number (RON) of 93. The total residual gas level was kept over 50% to achieve an engine-out NOx target of below 1.5 g/kWh. For a complete engine system analysis, one-dimensional (1-D) system-level modeling and three-dimensional (3-D) computational fluid dynamics (CFD) analysis were close-coupled in this study. Performance of the VVA strategies was compared in terms of in-cylinder charge and exhaust gas temperatures increase versus brake-specific fuel consumption (BSFC). The EEVO strategy demonstrated in-cylinder charge and exhaust temperature increase up to 130 and 180 K, respectively. For similar in-cylinder charge temperature gains, the ExReb strategy demonstrated 11% to 18% lower BSFC compared to the EEVO strategy. This benefit primarily originated from a more efficient gas-exchange process. The PVO strategy, due to the valve–piston contact constraint, required excessive exhaust back-pressure valve (BPV) throttling for hot residuals trapping, thereby incurring higher BSFC compared to ExReb. In addition, the ExReb strategy demonstrated the highest potential for exhaust temperature increase (up to 673 K) among the three strategies. This was achieved by ExReb’s maximum air-fuel ratio reduction from high internal residuals mass and BPV throttling. Finally, the ExReb profile was optimized in terms of the peak lift, the duration, and the location for maximizing the fuel-efficiency potential of the strategy.