bluuebunny/crossref_metadata_2025 huggingface dataset:
The Higher Education Act and Minority Serving Institutions: Towards a Typology of Title III and V Funded Programs
Authors: Boland William
Published: March 2018

To date, there has been little analysis of MSI Title III and V grant-funded programs across all MSI categories. For researchers, practitioners, and policymakers, it is imperative to explore the contributions of MSIs as manifested in Title III and V grant-funded programs. The purpose of this study is to analyze MSI Title III and V programs based on project abstracts. This study is driven by three research questions: How have MSIs used their Title III and V grants? What are the expressed outcomes of MSI grant funding? Using restricted-use data obtained from the U.S. Department of Education, NCES IPEDS, and the Office of Postsecondary Education, this study uses a quantitative content analysis strategy to respond to the research questions in ways that can help stakeholders begin to understand the impact of the federal grant on MSIs.
Novel Method for Rapidly Constructing Active Power Steady-State Security Regions Incorporating the Equivalent Reactances of TCSCs
Authors: Chen Rusi , Lin Tao , Chen Baoping , Bi Ruyu , Xu Xialing
Published: March 2018

Active power steady-state security regions (APSSRs), which can provide guidance for prevention and control through security checks, is of great importance for the safe operation of power systems in which more and more sustainable energy power generation is integrated. As a mature flexible AC transmission system (FACTS) device, thyristor-controlled series compensators (TCSCs) can carry out series compensation for the transmission line by controlling its equivalent reactance. With the change of the equivalent reactance parameter of a TCSC, the nodal admittance matrix and power flow distribution of the power system also changes. Inevitably, the APSSR will be different. Therefore, it is necessary and important to further incorporate the equivalent reactance parameters of TCSCs in the APSSR expression, which is generally established in the space of node active power injections. In this paper, a rapid construction method of APSSRs incorporating the equivalent reactances of TCSCs is proposed. Firstly, applicability and efficiency of the conventional APSSR construction method for power systems with TCSCs are analyzed. Further, with equivalent disconnection of TCSC branches, the effect of TCSC equivalent reactances on the distribution of active power flow through changing the structure parameters is treated as modifying node active power injections. On this basis, explicit expressions of APSSRs with a single TCSC equivalent reactance parameter and double TCSC equivalent reactance parameters are derived, respectively. Moreover, by deducing the general formula of APSSRs with multiple TCSC equivalent reactance parameters, the feasibility of the proposed method for power systems with multiple TCSCs is analyzed. Eventually, via benchmarks with different scales and a different number of TCSCs, validity and superiorities of the proposed method in computational efficiency are demonstrated.
An Experimental Investigation of Hydraulic Fracturing in Shale Considering Anisotropy and Using Freshwater and Supercritical CO2
Authors: He Jianming , Afolagboye Lekan , Lin Chong , Wan Xiaole
Published: March 2018

The process of hydraulic fracturing makes use of a liquid to fracture reservoir rocks for the exploitation of unconventional resources. Hence, it is vital to understand the processes that produce the fracture networks that occur during hydraulic fracturing. A shale reservoir is one of the largest unconventional resources and it displays obvious anisotropic characteristics due to its inherent sedimentary structures. The viscosity and flow ability of the fracturing fluid plays an important role in this process. We conducted a series of hydraulic fracturing tests on shale cores (from the southern Sichuan Basin) using freshwater and supercritical CO2 (SCO2) as fracturing fluids to investigate the different modes of fracture propagation. The pump pressure curves that we obtained during the fracturing experiment show how the shale responded to each of the fracturing fluids. We examined the influence of the anisotropic characteristics on the propagation of hydraulic fractures by conducting a series of hydraulic fracturing experiments on the shale cores using different bedding orientations. The bedding orientation of the shale had a profound influence on the fracture propagation when using either freshwater or a SCO2 fluid. The breakdown pressure of the shale core was affected not only by the bedding orientation but also by the fracturing fluid. A macroscopic observation of the fractures revealed different fracture geometries and propagation patterns. The results demonstrated that the anisotropic structures and the fracturing fluids could influence the path of the hydraulic fracture.
Automatic Identification of Fractures Using a Density-Based Clustering Algorithm with Time-Spatial Constraints
Authors: Xue Qingfeng , Wang Yibo , Zhai Hongyu , Chang Xu
Published: March 2018

In shale gas hydraulic fracture monitoring or rock acoustic emission experiments, fracture plane identification is always a complex task. Conventional approaches typically use the source locating results derived from the micro-seismic event and then interpret the fracture plane using manual qualitative analysis. Large errors typically occur due to manual operations. On the other hand, the density-based clustering algorithm with spatial constraints is widely used in geographic information science, biological cells science and astronomy. It is an automated algorithm and can achieve good classification results. In this paper, we introduced the above-mentioned clustering algorithm with spatial constraints to fracture identification applications. Moreover, because micro-seismic events are 4D in nature, every micro-seismic event has both time and space information. Hence, we improve the conventional clustering algorithm by incorporating a time constraint. We test the proposed method using rock acoustic emissions data and compare our fracture identification results with CT scan images; the comparison clearly shows the effectiveness of the proposed method.
Plant Water Use Strategy in Response to Spatial and Temporal Variation in Precipitation Patterns in China: A Stable Isotope Analysis
Authors: Zhao Ying , Wang Li
Published: March 2018

Spatial and temporal variation in precipitation patterns can directly alter the survival and growth of plants, yet in China there is no comprehensive and systematic strategy for plant use based on the effects of precipitation patterns. Here, we examined information from 93 published papers (368 plant species) on plant xylem water stable isotopes (δD and δ18O) in China. The results showed that: (1) The slope of the local meteoric water line (LMWL) gradually increased from inland areas to the coast, as a result of continental and seasonal effects. The correlation between δD and δ18O in plant stem water is also well fitted and the correlation coefficients range from 0.78 to 0.89. With respect to the soil water line, the δ18O values in relation to depth (0–100 cm) varied over time; (2) Plants’ main water sources are largely affected by precipitation patterns. In general, plants prioritize the use of stable and continuous water sources, while they have a more variable water uptake strategy under drought conditions; (3) There are no spatial and temporal variations in the contribution of the main water source (p > 0.05) because plants maintain growth by shifting their use of water sources when resources are unreliable.
Coordinating the Uncoordinated: The EU Forest Strategy
Authors: Aggestam Filip , Pülzl Helga
Published: March 2018

The second European Union (EU) Forest Strategy responds to new challenges facing both forests and the forest-based sector which highlights the EU’s need for a policy framework ensuring coordination and coherence of forest-related policies. The objective of the present article is to analyse whether the new Strategy contributes towards horizontal policy coherence of EU forest-related policies, given its shared and exclusive competences. This is achieved by comparing European Commission and forest industry policy priorities as articulated in the Strategy and through research carried out for the recent Cumulative Cost Assessment (CCA) of forest-based industries. Results from the comparative analysis demonstrate that the Strategy does not address many EU policies and policy instruments that affect the whole forest value chain and that it clearly omits existing EU policy instrument objectives that entail significant costs for the forest-based industry. It is therefore argued that without coordinating collective EU goals and gathering strong political support, it is at best extremely difficult or at worst impossible, to achieve coherence for EU forest-related policies across the whole forest value chain. Improving coherence of Union forest-related policies will require the Strategy to address more policy areas and instruments, including clearly defined parameters of what constitutes an EU forest-related policy. These pressing needs reach beyond what the Strategy presently sets out to achieve.
A Review of Methodology for Evaluating the Performance of Atmospheric Transport and Dispersion Models and Suggested Protocol for Providing More Informative Results
Authors: Herring Steven , Huq Pablo
Published: March 2018

Many models exist for predicting the atmospheric transport and dispersion of material following its release into the atmosphere. The purpose of these models may be to support air quality assessments and/or to predict the hazard resulting from releases of harmful materials to inform emergency response actions. In either case it is essential that the user understands the level of predictive accuracy that might be expected. However, contrary to expectation, this is not easily determined from published comparisons of model predictions against data from dispersion experiments. The paper presents and reviews the methods adopted and issues involved in comparing the predictive performance of atmospheric transport and dispersion models to experimental data, by reference to a number of experimental data sets and comparison results. It then presents an approach which is designed to make the performance of atmospheric dispersion models more transparent, through clearly defining the basis on which the comparison is made, and comparing the performance of the chosen model to that of a reference model. Such an approach establishes a clear baseline against which the accuracy of models can be evaluated and the performance benefits of more sophisticated approaches quantified. The use of a simple analytic reference model applicable to continuous ground level releases in open terrain and urban areas is shown as a proof-of-principle.
Multiscale Stuart-Landau Emulators: Application to Wind-Driven Ocean Gyres
Authors: Kondrashov Dmitri , Chekroun Mickaël , Berloff Pavel
Published: March 2018

The multiscale variability of the ocean circulation due to its nonlinear dynamics remains a big challenge for theoretical understanding and practical ocean modeling. This paper demonstrates how the data-adaptive harmonic (DAH) decomposition and inverse stochastic modeling techniques introduced in (Chekroun and Kondrashov, (2017), Chaos, 27), allow for reproducing with high fidelity the main statistical properties of multiscale variability in a coarse-grained eddy-resolving ocean flow. This fully-data-driven approach relies on extraction of frequency-ranked time-dependent coefficients describing the evolution of spatio-temporal DAH modes (DAHMs) in the oceanic flow data. In turn, the time series of these coefficients are efficiently modeled by a family of low-order stochastic differential equations (SDEs) stacked per frequency, involving a fixed set of predictor functions and a small number of model coefficients. These SDEs take the form of stochastic oscillators, identified as multilayer Stuart–Landau models (MSLMs), and their use is justified by relying on the theory of Ruelle–Pollicott resonances. The good modeling skills shown by the resulting DAH-MSLM emulators demonstrates the feasibility of using a network of stochastic oscillators for the modeling of geophysical turbulence. In a certain sense, the original quasiperiodic Landau view of turbulence, with the amendment of the inclusion of stochasticity, may be well suited to describe turbulence.
The Non-Coding Regulatory RNA Revolution in Archaea
Authors: Gelsinger Diego , DiRuggiero Jocelyne
Published: March 2018

Small non-coding RNAs (sRNAs) are ubiquitously found in the three domains of life playing large-scale roles in gene regulation, transposable element silencing and defense against foreign elements. While a substantial body of experimental work has been done to uncover function of sRNAs in Bacteria and Eukarya, the functional roles of sRNAs in Archaea are still poorly understood. Recently, high throughput studies using RNA-sequencing revealed that sRNAs are broadly expressed in the Archaea, comprising thousands of transcripts within the transcriptome during non-challenged and stressed conditions. Antisense sRNAs, which overlap a portion of a gene on the opposite strand (cis-acting), are the most abundantly expressed non-coding RNAs and they can be classified based on their binding patterns to mRNAs (3′ untranslated region (UTR), 5′ UTR, CDS-binding). These antisense sRNAs target many genes and pathways, suggesting extensive roles in gene regulation. Intergenic sRNAs are less abundantly expressed and their targets are difficult to find because of a lack of complete overlap between sRNAs and target mRNAs (trans-acting). While many sRNAs have been validated experimentally, a regulatory role has only been reported for very few of them. Further work is needed to elucidate sRNA-RNA binding mechanisms, the molecular determinants of sRNA-mediated regulation, whether protein components are involved and how sRNAs integrate with complex regulatory networks.
Integration of lncRNA and mRNA Transcriptome Analyses Reveals Genes and Pathways Potentially Involved in Calf Intestinal Growth and Development during the Early Weeks of Life
Authors: Ibeagha-Awemu Eveline , Do Duy , Dudemaine Pier-Luc , Fomenky Bridget , Bissonnette Nathalie
Published: March 2018

A better understanding of the factors that regulate growth and immune response of the gastrointestinal tract (GIT) of calves will promote informed management practices in calf rearing. This study aimed to explore genomics (messenger RNA (mRNA)) and epigenomics (long non-coding RNA (lncRNA)) mechanisms regulating the development of the rumen and ileum in calves. Thirty-two calves (≈5-days-old) were reared for 96 days following standard procedures. Sixteen calves were humanely euthanized on experiment day 33 (D33) (pre-weaning) and another 16 on D96 (post-weaning) for collection of ileum and rumen tissues. RNA from tissues was subjected to next generation sequencing and 3310 and 4217 mRNAs were differentially expressed (DE) between D33 and D96 in ileum and rumen tissues, respectively. Gene ontology and pathways enrichment of DE genes confirmed their roles in developmental processes, immunity and lipid metabolism. A total of 1568 (63 known and 1505 novel) and 4243 (88 known and 4155 novel) lncRNAs were detected in ileum and rumen tissues, respectively. Cis target gene analysis identified BMPR1A, an important gene for a GIT disease (juvenile polyposis syndrome) in humans, as a candidate cis target gene for lncRNAs in both tissues. LncRNA cis target gene enrichment suggested that lncRNAs might regulate growth and development in both tissues as well as posttranscriptional gene silencing by RNA or microRNA processing in rumen, or disease resistance mechanisms in ileum. This study provides a catalog of bovine lncRNAs and set a baseline for exploring their functions in calf GIT development.
Cell-Free Approaches in Synthetic Biology Utilizing Microfluidics
Authors: Damiati Samar , Mhanna Rami , Kodzius Rimantas , Ehmoser Eva-Kathrin
Published: March 2018

Synthetic biology is a rapidly growing multidisciplinary branch of science which aims to mimic complex biological systems by creating similar forms. Constructing an artificial system requires optimization at the gene and protein levels to allow the formation of entire biological pathways. Advances in cell-free synthetic biology have helped in discovering new genes, proteins, and pathways bypassing the complexity of the complex pathway interactions in living cells. Furthermore, this method is cost- and time-effective with access to the cellular protein factory without the membrane boundaries. The freedom of design, full automation, and mimicking of in vivo systems reveal advantages of synthetic biology that can improve the molecular understanding of processes, relevant for life science applications. In parallel, in vitro approaches have enhanced our understanding of the living system. This review highlights the recent evolution of cell-free gene design, proteins, and cells integrated with microfluidic platforms as a promising technology, which has allowed for the transformation of the concept of bioprocesses. Although several challenges remain, the manipulation of biological synthetic machinery in microfluidic devices as suitable ‘homes’ for in vitro protein synthesis has been proposed as a pioneering approach for the development of new platforms, relevant in biomedical and diagnostic contexts towards even the sensing and monitoring of environmental issues.
One-Year Mortality Rates Before and After Implementing Quality-Improvement Initiatives to Prevent Inpatient Falls (2012–2016)
Authors: Singh Inderpal , Edwards Chris , Anwar Anser
Published: March 2018

Single-room ward design has previously been associated with increased risk of inpatient falls and adverse outcomes. However, following quality initiatives, the incidence of inpatient falls has shown a sustained reduction. Benefits have also been observed in the reduction of hip fractures. However, one-year mortality trends have not been reported. The aim of this observational study is to report the trends in one-year mortality rates before and after implementing quality-improvement initiatives to prevent inpatient falls over the last 5 years (2012–2016). This retrospective observational study was conducted for all patients who had sustained an inpatient fall between January 2012 and December 2016. All the incident reports in DATIX patient-safety software which were completed for each inpatient fall were studied, and the clinical information was extracted from Clinical Work Station software. Mortality data were collected on all patients for a minimum of one year following the discharge from the hospital. The results show that 95% patients were admitted from their own homes; 1704 patients had experienced 3408 incidents of an inpatient fall over 5 years. The mean age of females (82.61 ± 10.34 years) was significantly higher than males (79.36 ± 10.14 years). Mean falls/patient = 2.0 ± 2.16, range 1–33). Mean hospital stay was 45.43 ± 41.42 days. Mean hospital stay to the first fall was 14.5 ± 20.79 days, and mean days to first fall prior to discharge was 30.8 ± 34.33 days. The results showed a significant and sustained reduction in the incidence of inpatient falls. There was a downward trend in the incidence of hip fractures over the last two years. There was no significant difference in the inpatient and 30-day mortality rate over the last five years. However, mortality trends appear to show a significant downward trend in both six-month and one-year mortality rates over the last two years following the implementation of quality initiatives to prevent inpatient falls. A significant reduction in the incidence of inpatient falls following quality initiatives initially has been observed, followed by a downward trend in the incidence of hip fractures. We have just started to observe a significant reduction in the 6-month and one-year mortality. We propose prompt completion of multifactorial falls risk assessments, and every possible quality initiative should be taken to prevent a ‘first inpatient fall’, which should result in the sustained improvement of clinical outcomes.
Effect of Paclobutrazol Application on Plant Photosynthetic Performance and Leaf Greenness of Herbaceous Peony
Authors: Xia Xing , Tang Yuhan , Wei Mengran , Zhao Daqiu
Published: March 2018

Paclobutrazol (PBZ) has been associated with effects on the photosynthetic capacity of plants. PBZ affects the growth and development of plants in general. However, little is known about the effects of PBZ on photosynthetic performance and related anatomical features of herbaceous peony (Paeonia lactiflora Pall.) leaves. In the present study, PBZ application resulted in a significant reduction in peony plant height. Furthermore, PBZ application significantly increased photosynthetic rate (Pn), transpiration rate (Tr) and water use efficiency (WUE), but significantly decreased intercellular CO2 concentration (Ci) at some stages from the bolting stage to the bud stage of the plants, compared to controls. Moreover, PBZ application increased the maximum quantum yield of PSII photochemistry (Fv/Fm), coefficient of photochemical quenching (qP) and intrinsic PSII efficiency (ΦPSII), but decreased the coefficient of non-photochemical quenching (qN) and non-photochemical quenching (NPQ). Leaves treated with PBZ had a heavy aggregation of chloroplasts close to the cell wall, with distinct grana lamellae, more and bigger starch grains (on average for a chloroplast), and fewer plastoglobuli, as compared to the control. PBZ increased chlorophyll content (SPAD) and the number of chloroplasts in individual cells in the foliar ultrastructure. PBZ-treated leaves had a darker green color with decreased luminosity (L*) and increased hue angle (h°). The results indicated that plants treated with PBZ were superior in terms of increased photosynthetic characteristics when compared with untreated controls. The direct cause of the increase in Pn and leaf greenness of PBZ-treated P. lactiflora may be the increase in chlorophyll content.
Hygiene and Health: Who Do Mothers in Vanuatu Communicate with about Health?
Authors: File Karen , Valente Thomas , McLaws Mary-Louise
Published: March 2018

Health information-seeking behaviour of mothers with children five years of age and younger in Vanuatu was examined using the structural properties of social networks. Data were collected from a rural village from two islands and an urban settlement in the capital, Port Vila, by face-to-face interviews using a structured questionnaire. Sociometric data on the structure of the network, the characteristics of key informants, and associations with outside sources of health information were analysed as interpersonal predictors of health promotion and behavior change. Rural mothers preferred the health advice of biomedical practitioners for diarrheal disease over traditional custom practitioners. Interpersonal connections were restricted in the urban mother network indicating that mothers were merely acquaintances or do not seek health advice from each other. Our findings suggest that biomedical practitioners are the best option for diffusing health and hygiene information for rural and urban mothers. Traditional healers and paraprofessionals could be strategically used to complete the missing links in network connectedness to optimally spread new information. The novel use of cross-sectional social network data can create a baseline evaluation to purposefully frame a health intervention. Our study provided a unique explanation of how network analysis offers insight into how key players can be identified and the circumstances in which they are likely to be able to influence hygiene practices of their peers.
Gas Storage Valuation and Hedging: A Quantification of Model Risk
Authors: Hénaff Patrick , Laachir Ismail , Russo Francesco
Published: March 2018

This paper focuses on the valuation and hedging of gas storage facilities, using a spot-based valuation framework coupled with a financial hedging strategy implemented with futures contracts. The contributions of this paper are two-fold. Firstly, we propose a model that unifies the dynamics of the futures curve and spot price, and accounts for the main stylized facts of the US natural gas market such as seasonality and the presence of price spikes in the spot market. Secondly, we evaluate the associated model risk, and show not only that the valuation is strongly dependent upon the dynamics of the spot price, but more importantly that the hedging strategy commonly used in the industry leaves the storage operator with significant residual price risk.
Functional Characterization of the Steroid Reductase Genes GmDET2a and GmDET2b from Glycine max
Authors: Huo Weige , Li Bodi , Kuang Jiebing , He Pingan , Xu Zhihao , Wang Jinxiang
Published: March 2018

Brassinosteroids are important phytohormones for plant growth and development. In soybean (Glycine max), BR receptors have been identified, but the genes encoding BR biosynthesis-related enzymes remain poorly understood. Here, we found that the soybean genome encodes eight steroid reductases (GmDET2a to GmDET2h). Phylogenetic analysis grouped 105 steroid reductases from moss, fern and higher plants into five subgroups and indicated that the steroid reductase family has experienced purifying selection. GmDET2a and GmDET2b, homologs of the Arabidopsis thaliana steroid 5 α -reductase AtDET2, are proteins of 263 amino acids. Ectopic expression of GmDET2a and GmDET2b rescued the defects of the Atdet2-1 mutant in both darkness and light. Compared to the mutant, the hypocotyl length and plant height of the transgenic lines GmDET2a and GmDET2b increased significantly, in both darkness and light, and the transcript levels of the BR biosynthesis-related genes CPD, DWF4, BR6ox-1 and BR6ox-2 were downregulated in GmDET2aOX-23 and GmDET2bOX-16 lines compared to that in Atdet2-1. Quantitative real-time PCR revealed that GmDET2a and GmDET2b are ubiquitously expressed in all tested soybean organs, including roots, leaves and hypocotyls. Moreover, epibrassinosteroid negatively regulated GmDET2a and GmDET2b expression. Sulfate deficiency downregulated GmDET2a in leaves and GmDET2b in leaves and roots; by contrast, phosphate deficiency upregulated GmDET2b in roots and leaves. Taken together, our results revealed that GmDET2a and GmDET2b function as steroid reductases.
Nanotechnology and Glycosaminoglycans: Paving the Way Forward for Ovarian Cancer Intervention
Authors: Hoosen Yasar , Pradeep Priyamvada , Kumar Pradeep , Du Toit Lisa , Choonara Yahya , Pillay Viness
Published: March 2018

Ovarian cancer (OC) has gained a great deal of attention due to its aggressive proliferative capabilities, high death rates and poor treatment outcomes, rendering the disease the ultimate lethal gynaecological cancer. Nanotechnology provides a promising avenue to combat this malignancy by the niche fabrication of optimally-structured nanomedicines that ensure potent delivery of chemotherapeutics to OC, employing nanocarriers to act as “intelligent” drug delivery vehicles, functionalized with active targeting approaches for precision delivery of chemotherapeutics to overexpressed biomarkers on cancer cells. Recently, much focus has been implemented to optimize these active targeting mechanisms for treatment/diagnostic purposes employing nanocarriers. This two-part article aims to review the latest advances in active target-based OC interventions, where the impact of the newest antibody, aptamer and folate functionalization on OC detection and treatment is discussed in contrast to the limitations of this targeting mechanism. Furthermore, we discuss the latest advances in nanocarrier based drug delivery in OC, highlighting their commercial/clinical viability of these systems beyond the realms of research. Lastly, in the second section of this review, we comprehensively discussed a focus shift in OC targeting from the well-studied OC cells to the vastly neglected extracellular matrix and motivate the potential for glycosaminoglycans (GAGs) as a more focused extracellular molecular target.
Regulation of Ion Transport in the Intestine by Free Fatty Acid Receptor 2 and 3: Possible Involvement of the Diffuse Chemosensory System
Authors: Kuwahara Atsukazu , Kuwahara Yuko , Inui Toshio , Marunaka Yoshinori
Published: March 2018

The diffuse chemosensory system (DCS) is well developed in the apparatuses of endodermal origin like gastrointestinal (GI) tract. The primary function of the GI tract is the extraction of nutrients from the diet. Therefore, the GI tract must possess an efficient surveillance system that continuously monitors the luminal contents for beneficial or harmful compounds. Recent studies have shown that specialized cells in the intestinal lining can sense changes in the luminal content. The chemosensory cells in the GI tract belong to the DCS which consists of enteroendocrine and related cells. These cells initiate various important local and remote reflexes. Although neural and hormonal involvements in ion transport in the GI tract are well documented, involvement of the DCS in the regulation of intestinal ion transport is much less understood. Since activation of luminal chemosensory receptors is a primary signal that elicits changes in intestinal ion transport and motility and failure of the system causes dysfunctions in host homeostasis, as well as functional GI disorders, study of the regulation of GI function by the DCS has become increasingly important. This review discusses the role of the DCS in epithelial ion transport, with particular emphasis on the involvement of free fatty acid receptor 2 (FFA2) and free fatty acid receptor 3 (FFA3).
Effect of Presence and Concentration of Plasticizers, Vegetable Oils, and Surfactants on the Properties of Sodium-Alginate-Based Edible Coatings
Authors: Senturk Parreidt Tugce , Schott Michael , Schmid Markus , Müller Kajetan
Published: March 2018

Achieving high quality of a coated food product is mostly dependent on the characteristics of the food material to be coated, the properties of the components in the coating solution, and the obtained coating material. In the present study, usability and effectiveness of various components as well as their concentrations were assessed to produce an effective coating material. For this purpose, different concentrations of gelling agent (sodium alginate 0–3.5%, w/w), plasticizers (glycerol and sorbitol (0–20%, w/w), surfactants (tween 40, tween 80, span 60, span 80, lecithin (0–5%, w/w), and vegetable oils (sunflower oil, olive oil, rapeseed oil (0–5%, w/w) were used to prepare edible coating solutions. Formulations were built gradually, and characteristics of coatings were evaluated by analyzing surface tension values and its polar and dispersive components, emulsion droplet size, and optical appearance in microscopic scale. The results obtained showed that 1.25% sodium alginate, 2% glycerol, 0.2% sunflower oil, 1% span 80, and 0.2% tween 40 or tween 80 can be used in formulation to obtain an effective coating for hydrophobic food surfaces. Three formulations were designed, and their stability (emulsion droplet size, optical characteristics, and creaming index) and wettability tests on strawberry showed that they could be successfully used in coating applications.
The Setup Design for Selective Laser Sintering of High-Temperature Polymer Materials with the Alignment Control System of Layer Deposition
Authors: Nazarov Alexey , Skornyakov Innokentiy , Shishkovsky Igor
Published: March 2018

This paper presents the design of an additive setup for the selective laser sintering (SLS) of high-temperature polymeric materials, which is distinguished by an original control system for aligning the device for depositing layers of polyether ether ketone (PEEK) powder. The kinematic and laser-optical schemes are given. The main cooling circuits are described. The proposed technical and design solutions enable conducting the SLS process in different types of high-temperature polymer powders. The principles of the device adjustment for depositing powder layers based on an integral thermal analysis are disclosed. The PEEK sinterability was shown on the designed installation. The physic-mechanical properties of the tested 3D parts were evaluated in comparison with the known data and showed an acceptable quality.
Preliminary Results on the Evaluation of the Occurrence of Tetrodotoxin Associated to Marine Vibrio spp. in Bivalves from the Galician Rias (Northwest of Spain)
Authors: Leão Jose Manuel , Lozano-Leon Antonio , Giráldez Jorge , Vilariño Óscar , Gago-Martínez Ana
Published: March 2018

Tetrodotoxins (TTX) are a potent group of natural neurotoxins putatively produced by symbiotic microorganisms and affecting the aquatic environment. These neurotoxins have been recently found in some species of bivalves and gastropods along the European Coasts (Greece, UK, and The Netherlands) linked to the presence of high concentrations of Vibrio, in particular Vibrio parahaemolyticus. This study is focused on the evaluation of the presence of Vibrio species and TTX in bivalves (mussels, oysters, cockles, clams, scallops, and razor clams) from Galician Rias (northwest of Spain). The detection and isolation of the major Vibrio spp. and other enterobacterial populations have been carried out with the aim of screening for the presence of the pathways genes, poliketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) possibly involved in the biosynthesis of these toxins. Samples containing Vibrio spp. were analyzed by biochemical (API20E-galery) and genetic tests (PCR-RT). These samples were then screened for TTX toxicity by a neuroblastoma cell-based assay (N2a) and the presence of TTX was further confirmed by LC-MS/MS. TTX was detected in two infaunal samples. This is the first confirmation of the presence of TTX in bivalve molluscs from the Galician Rias.
Risk Factors Including Age, Stage and Anatomic Location that Impact the Outcomes of Patients with Synovial Sarcoma
Authors: Pan Minggui , Merchant Maqdooda
Published: March 2018

Synovial sarcoma is a high-grade soft tissue sarcoma that inflicts mostly children and young adults with high mortality rate; however, the risk factors that impact the outcomes remain incompletely understood. We have identified the synovial sarcoma cases from the Kaiser Permanente Northern California cancer registry between 1981 and 2014. Kaplan–Meier plots were used to display disease-free survival (DFS) and overall survival (OS); log-rank tests and Cox proportional hazard models were used to determine the impact of clinical factors on DFS, OS, and disease-specific survival. Tumor size > 5.0 cm and age > 50 years were associated with higher risk of presenting with stage IV disease. Median OS for patients with stage IV was 1.3 years and 7.8 years for early-stage disease. For patients with early-stage disease, tumor size > 5.0 cm was significantly associated with worse DFS, sarcoma-specific morality, and OS. Compared to extremity primary, patients with head and neck and trunk primary had approximately three-fold higher sarcoma-specific mortality and lower OS. There was no significant difference in DFS or OS among three histologic subtypes. Pre-operative and/or post-operative chemotherapy was not associated with improvement in DFS or OS. Twenty-six patients relapsed with predominantly lung metastasis, thirteen of whom received metastatectomy with a median OS of 7.8 years, compared to 2.3 years for patients who did not receive metastatectomy. In conclusion, age older than 50 years and tumor size > 5.0 cm are risk factors for presenting with stage IV disease. For early-stage patients, trunk and head and neck primary as well as tumor size > 5.0 cm are risk factors for decreased OS.
Interface Characteristic of Explosive-Welded and Hot-Rolled TA1/X65 Bimetallic Plate
Authors: Xie Miao-Xia , Shang Xiang-Tao , Zhang Lin-Jie , Bai Qing-Lin , Xu Ting-Ting
Published: March 2018

TA1/X65 bimetallic plate has a bright future of application by combining the excellent corrosion resistance of TA1 and the high strength of inexpensive X65 steel, while manufacturing large size TA1/X65 bimetallic plate is still a challenging task. Multi-pass hot-rolling is the most common way to achieve a large size bimetallic plate. In this work, interface characteristic of explosive-welded and multi-pass hot-rolled TA1/X65 bimetallic plate is experimentally studied. The microstructure, composition and microhardness distribution across the TA1/X65 interface are investigated by optical metallographic observation, scanning electron microscope (SEM) observation, energy dispersive spectrometer (EDS) analysis, and Vickers hardness test. Shear tests and stratified tensile tests are conducted with emphasis on impacts of the angle between loading direction and detonation wave propagation direction on interface strength. A straight TA1/X65 interface with periodic morphology of residual peninsula could be observed on the cross section parallel to detonation wave propagation direction, while in most cases there is no residual peninsula morphology on the straight TA1/X65 interface when the cross section is perpendicular to detonation wave propagation direction. TA1/X65 interface of explosive-welded and multi-pass hot-rolled bimetallic plate presents higher bearing capacity for the load perpendicular to detonation wave propagation direction than that for the load parallel to detonation wave propagation direction. The results of this paper have a certain guiding significance for the fabrication of pipes and containers made of explosive-welded and hot-rolled TA1/X65 bimetallic plate.
Dissolved Silica Effects on Adsorption and Co-Precipitation of Sb(III) and Sb(V) with Ferrihydrite
Authors: Zhou Shuang , Sato Tsutomu , Otake Tsubasa
Published: March 2018

Elevated antimony concentrations in aqueous environments from anthropogenic sources are becoming of global concern. In this respect iron oxides are known to strongly adsorb aqueous antimony species with different oxidation states, but the effect of silica on the removal characteristics is not well understood despite being a common component in the environment. In this study, ferrihydrite was synthesized at various Si/Fe molar ratios to investigate its adsorption and co-precipitation behaviors with aqueous antimony anionic species, Sb(III) and Sb(V). The X-ray diffraction analyses of the precipitates showed two broad diffraction features at approximately 35° and 62° 2θ, which are characteristics of 2-line ferrihydrite, but no significant shifts in peak positions in the ferrihydrite regardless of the Si/Fe ratios. The infrared spectra showed a sharp band at ~930 cm−1, corresponding to asymmetric stretching vibrations of Si–O–Fe bonds which increased in intensity with increasing Si/Fe molar ratios. Further, the surface charge on the precipitates became more negative with increasing Si/Fe molar ratios. The adsorption experiments indicated that Sb(V) was preferentially adsorbed under acidic conditions which decreased dramatically with increasing pH while the adsorption rate of Sb(III) ions was independent of pH. However, the presence of silica suppressed the adsorption of both Sb(III) and Sb(V) ions. The results showed that Sb(III) and Sb(V) ions were significantly inhibited by co-precipitation with ferrihydrite even in the presence of silica by isomorphous substitution in the ferrihydrite crystal structure.
Network Formation via Anion Coordination: Crystal Structures Based on the Interplay of Non-Covalent Interactions
Authors: Savastano Matteo , Bazzicalupi Carla , Mariani Palma , Bianchi Antonio
Published: March 2018

We describe the synthesis and the structural characterization of new H2L(CF3CO2)2 (1) and H2L(Ph2PO4)2 (2) compounds containing the diprotonated form (H2L2+) of the tetrazine-based molecule 3,6-di(pyridin-4-yl)-1,2,4,5-tetrazine. X-ray diffraction (XRD) analysis of single crystals of these compounds showed that H2L2+ displays similar binding properties toward both anions when salt bridge interactions are taken into account. Nevertheless, the different shapes, sizes and functionalities of trifluoroacetate and diphenyl phosphate anions define quite different organization patterns leading to the peculiar crystal lattices of 1 and 2. These three-dimensional (3D) architectures are self-assembled by a variety of non-covalent forces, among which prominent roles are played by fluorine–π (in 1) and anion–π (in 2) interactions.
Quality Evaluation of Apocyni Veneti Folium from Different Habitats and Commercial Herbs Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis
Authors: Chen Cuihua , Liu Zixiu , Zou Lisi , Liu Xunhong , Chai Chuan , Zhao Hui , Yan Ying , Wang Chengcheng
Published: March 2018

Apocyni Veneti Folium (AVF) is a kind of staple traditional Chinese medicine with vast clinical consumption because of its positive effects. However, due to the habitats and adulterants, its quality is uneven. To control the quality of this medicinal herb, in this study, the quality of AVF was evaluated based on simultaneous determination of multiple bioactive constituents combined with multivariate statistical analysis. A reliable method based on ultra-fast liquid chromatography tandem triple quadrupole mass spectrometry (UFLC-QTRAP-MS/MS) was developed for the simultaneous determination of a total of 43 constituents, including 15 flavonoids, 6 organic acids, 13 amino acids, and 9 nucleosides in 41 Luobumaye samples from different habitats and commercial herbs. Furthermore, according to the contents of these 43 constituents, principal component analysis (PCA) was employed to classify and distinguish between AVF and its adulterants, leaves of Poacynum hendersonii (PHF), and gray relational analysis (GRA) was performed to evaluate the quality of the samples. The proposed method was successfully applied to the comprehensive quality evaluation of AVF, and all results demonstrated that the quality of AVF was higher than the PHF. This study will provide comprehensive information necessary for the quality control of AVF.
GC-MS Analysis of the Composition of the Essential Oil from Dendranthema indicum Var. Aromaticum Using Three Extraction Methods and Two Columns
Authors: Fan Sanpeng , Chang Jin , Zong Yufeng , Hu Gaosheng , Jia Jingming
Published: March 2018

Dendranthema indicum var. aromaticum, which is an aromatic plant with a strong and special fragrance throughout the whole plant, is used for the treatment of colds and headaches, and as a mosquito repellant in Shennongjia, Hubei province, China. To analyze the composition of the essential oil from this medicinal herb, we developed a gas chromatography-mass Spectrometry (GC-MS) method including microwave-assisted extraction, hydrodistillation and direct headspace analysis in two different stationary phase columns. In total, 115 volatile compounds were identified, of which 90 compounds were identified using Rxi-5MS and 78 using HP-INNOWAX. Our results revealed that the oil was mainly composed of five categories of compound: oxygenated monoterpenes (28.76–78.10%), oxygenated sesquiterpenes (4.27–38.06%), sesquiterpenes (3.22–11.57%), fatty hydrocarbons (1.65–9.81%) and monoterpenes (0–3.32%). The major constituents are α-thujone, β-thujone, cis-sabinol, sabinyl acetate and (-)-neointermedeol.However, the essential oil composition in the published literature differs significantly. Therefore, a cluster analysis was carried out using the top ten compositions in the reported literature as well as this study, using Minitab software. To provide detailed information on plant origin, the ITS1-5.8s-ITS2 region was amplified and sequenced (Accession No. MF668250). Besides, in order to provide a macroscopic view of the chemical composition, the biosynthetic pathway of the main components was summarized according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and the published literatures.
Quantum Chemical Design Guidelines for Absorption and Emission Color Tuning of fac-Ir(ppy)3 Complexes
Authors: Natori Yoshiki , Kitagawa Yasutaka , Aoki Shogo , Teramoto Rena , Tada Hayato , Era Iori , Nakano Masayoshi
Published: March 2018

The fac-Ir(ppy)3 complex, where ppy denotes 2-phenylpyridine, is one of the well-known luminescent metal complexes having a high quantum yield. However, there have been no specific molecular design guidelines for color tuning. For example, it is still unclear how its optical properties are changed when changing substitution groups of ligands. Therefore, in this study, differences in the electronic structures and optical properties among several substituted fac-Ir(ppy)3 derivatives are examined in detail by density functional theory (DFT) and time-dependent DFT (TD-DFT) calculations. On the basis of those results, we present rational design guidelines for absorption and emission color tuning by modifying the species of substituents and their substitution positions.
Melatonin Improves Heat Tolerance in Kiwifruit Seedlings through Promoting Antioxidant Enzymatic Activity and Glutathione S-Transferase Transcription
Authors: Liang Dong , Gao Fan , Ni Zhiyou , Lin Lijin , Deng Qunxian , Tang Yi , Wang Xun , Luo Xian , Xia Hui
Published: March 2018

Evidence exists to suggest that melatonin (MT) is important to abiotic stress tolerance in plants. Here, we investigated whether exogenous MT reduces heat damage on biological parameters and gene expression in kiwifruit (Actinidia deliciosa) seedlings. Pretreatment with MT alleviates heat-induced oxidative harm through reducing H2O2 content and increasing proline content. Moreover, MT application raised ascorbic acid (AsA) levels and the activity of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD). We also observed elevation in the activity of enzymes related to the AsA-GSH cycle, such as ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR). Furthermore, MT application increased the expression of 28/31 glutathione S-transferase (GST) genes, reducing oxidative stress. These results clearly indicate that in kiwifruit, MT exerts a protective effect against heat-related damage through regulating antioxidant pathways.
Special Issue: Antibacterial Materials and Coatings
Authors: Vasilev Krasimir , Cavallaro Alex , Zilm Peter
Published: March 2018

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Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus
Authors: Qin Yujing , Yuan Qingxia , Zhang Yuexing , Li Jialu , Zhu Xinjiao , Zhao Lingling , Wen Jing , Liu Jikai , Zhao Longyan , Zhao Jinhua
Published: March 2018

Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study. The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%. Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98Cl and Sepharose CL-6B columns. Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc. Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2 were determined to be 21.9%, 20.6%, 25.2% and 28.0% (w/w), respectively. PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2. PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.