bluuebunny/crossref_metadata_2025 huggingface dataset:
Income and Self-Rated Mental Health: Diminished Returns for High Income Black Americans
Authors: Assari Shervin , Lapeyrouse Lisa M. , Neighbors Harold W.
Published: May 2018

Background: The minorities’ diminished return theory suggests that socioeconomic position (SEP) generates smaller health gains for racial/ethnic minorities compared to Whites. The current study was a Black–White comparison of the association between household income and self-rated mental health (SRMH). Methods: This cross-sectional study used data from the 2017 State of the State Survey (SOSS). With representative sampling, the SOSS generates results that are generalizable to the state of Michigan. This study included 881 adults, (n = 92) Black and (n = 782) White. The independent variable was household income. The dependent variable was SRMH, measured using a single item. Age, gender, and participation in the labor force were covariates. Race/ethnicity was the focal moderator. Logistic regression models were used for data analysis. Results: Overall, higher household income was associated with better SRMH, net of covariates. An interaction was found between race/ethnicity and household income on SRMH, suggesting a smaller, or nonexistent, protective effect for Blacks compared to Whites. In race/ethnicity-stratified models, higher household income was associated with better SRMH for Whites but not Blacks. Conclusion: Supporting the minorities’ diminished return theory, our study documents differential effects for income on SRHM for Blacks and Whites, where Whites but not Blacks appear to benefit from their income. Given this, researchers and policy makers are cautioned against making assumptions that racial groups benefit equally from similar economic resources.
Temozolomide Enhances Triple-Negative Breast Cancer Virotherapy In Vitro
Authors: Garza-Morales Rodolfo , Gonzalez-Ramos Roxana , Chiba Akiko , Montes de Oca-Luna Roberto , McNally Lacey R. , McMasters Kelly M. , Gomez-Gutierrez Jorge G.
Published: May 2018

Triple-negative breast cancer (TNBC) is one of the most aggressive types of cancer, and treatment is limited to chemotherapy and radiation. Oncolytic virotherapy may be a promising approach to treat TNBC. However, oncolytic adenovirus (OAd)-based mono-therapeutic clinical trials have resulted in modest outcomes. The OAd potency could be increased by chemotherapy-induced autophagy, an intracellular degradation system that delivers cytoplasmic constituents to the lysosome. In this study, the ability of alkylating agent temozolomide (TMZ)-induced autophagy to increase OAd replication and oncolysis in TNBC cells was evaluated. Human TNBC MDA-MB-231 and HCC1937 cells and mouse 4T1 cells were infected with an OAd expressing the red fluorescent protein mCherry on the virus capsid (OAdmCherry) alone or in combination with TMZ. TNBC cells treated with OAdmCherry/TMZ displayed greater mCherry and adenovirus (Ad) early region 1A (E1A) expression and enhanced cancer-cell killing compared to OAdmCherry or TMZ alone. The combined therapy-mediated cell death was associated with virus replication and accumulation of the autophagy marker light chain 3 (LC3)-II. Overall, this study provides experimental evidence of TMZ’s ability to increase oncolytic virotherapy in both human and murine TNBC cells.
OGT (O-GlcNAc Transferase) Selectively Modifies Multiple Residues Unique to Lamin A
Authors: Simon Dan N. , Wriston Amanda , Fan Qiong , Shabanowitz Jeffrey , Florwick Alyssa , Dharmaraj Tejas , Peterson Sherket B. , Gruenbaum Yosef , Carlson Cathrine R. , Grønning-Wang Line M. , Hunt Donald F. , Wilson Katherine L.
Published: May 2018

The LMNA gene encodes lamins A and C with key roles in nuclear structure, signaling, gene regulation, and genome integrity. Mutations in LMNA cause over 12 diseases (‘laminopathies’). Lamins A and C are identical for their first 566 residues. However, they form separate filaments in vivo, with apparently distinct roles. We report that lamin A is β-O-linked N-acetylglucosamine-(O-GlcNAc)-modified in human hepatoma (Huh7) cells and in mouse liver. In vitro assays with purified O-GlcNAc transferase (OGT) enzyme showed robust O-GlcNAcylation of recombinant mature lamin A tails (residues 385–646), with no detectable modification of lamin B1, lamin C, or ‘progerin’ (Δ50) tails. Using mass spectrometry, we identified 11 O-GlcNAc sites in a ‘sweet spot’ unique to lamin A, with up to seven sugars per peptide. Most sites were unpredicted by current algorithms. Double-mutant (S612A/T643A) lamin A tails were still robustly O-GlcNAc-modified at seven sites. By contrast, O-GlcNAcylation was undetectable on tails bearing deletion Δ50, which causes Hutchinson–Gilford progeria syndrome, and greatly reduced by deletion Δ35. We conclude that residues deleted in progeria are required for substrate recognition and/or modification by OGT in vitro. Interestingly, deletion Δ35, which does not remove the majority of identified O-GlcNAc sites, does remove potential OGT-association motifs (lamin A residues 622–625 and 639–645) homologous to that in mouse Tet1. These biochemical results are significant because they identify a novel molecular pathway that may profoundly influence lamin A function. The hypothesis that lamin A is selectively regulated by OGT warrants future testing in vivo, along with two predictions: genetic variants may contribute to disease by perturbing OGT-dependent regulation, and nutrient or other stresses might cause OGT to misregulate wildtype lamin A.
The Rights of Children on the Move and the Budapest Declaration
Authors: Oberg Charles
Published: May 2018

It has been estimated that more than 50,000,000 children and youth have migrated across borders or been forcibly displaced within their own country. They consist of refugees, asylum seekers, internally displaced persons (IDP), economic migrants, and exploited trafficked children. They are virtually “stateless”, children deprived of the protective structures of state and family that they need and deserve and unrecognized by either their country of origin or the international community. This opinion piece starts with the personal reflections of its author on his recent work in Middle East refugee camps. It then explores the prevalence and demographics of these children and their plight. It examines the United Nation’s Convention on the Rights of the Child (CRC) and other international conventions designed to protect them. It also summarizes the International Society of Social Pediatrics and Child Health (ISSOP) Budapest Declaration on the Rights, Health andWell-Being of Children and Youth on the Move as a framework for improved care and vehicle for change.
Feasibility of Predicting Vietnam’s Autumn Rainfall Regime Based on the Tree-Ring Record and Decadal Variability
Authors: Sun Yan , Wang S. -Y. Simon , Li Rong , Buckley Brendan M. , Gillies Robert , Hansen Kyle G.
Published: May 2018

We investigate the feasibility of developing decadal prediction models for autumn rainfall ( R A ) over Central Vietnam by utilizing a published tree-ring reconstruction of October–November (ON) rainfall derived from the earlywood width measurements from a type of Douglas-fir (Pseudotsuga sinensis). Autumn rainfall for this region accounts for a large percentage of the annual total, and is often the source of extreme flooding. Central Vietnam’s R A is characterized by a pronounced decadal oscillation signal. We use the decadal mode of R A along with its notable autocorrelation and significant cross-correlation with basin-wide Pacific sea surface temperature (SST) variability, to develop four discrete time-series models. The sparse autoregressive model, with Pacific SST as an external variable, accounts for most of the autoregressive R A , while taking advantage of the predictability from the basin-wide Pacific climate oscillation. Using this model, the decadal prediction of R A can be reasonably achieved with a 10-year-ahead forecasting skill score (SS) about 0.46. We therefore suggest, with this paper, that forecasting R A for Central Vietnam for multiple years ahead is possible using a time-series model.
Absorption and Photo-Stability of Substituted Dibenzoylmethanes and Chalcones as UVA Filters
Authors: Quintana Lazópulos Silvina , Svarc Federico , Sagrera Gabriel , Dicelio Lelia
Published: May 2018

There is still an international need to develop broad-spectrum sunscreen products with an adequate UVB/UVA balance, while the approved filters available in the UVA are scarce. Currently, one of the few UVA filters approved in the United States and Europe is tert-butylmethoxydibenzoylmethane (BMDM, avobenzone). However, this compound is unstable from aphotochemical point of view and cannot be used in combination with certain sunscreens. In this paper, we investigate the photochemical behavior of a set of dibenzoylmethanes and chalcones. In particular, we carry out their absorption and emission spectra, evaluate their photochemical degradation, and study their generation of free radicals and singlet oxygen photoproduction. Two compounds resultedin having the basic properties of UVA filters (2′-hydroxy-4-methoxychalcone and 2′-hydroxy-4-methoxydibenzoylmethane). Further studies are proposed, such as formulating the compounds into emulsions or other common cosmetic presentations, as well as combining them with broadly-used UVB filters. We have also considered the need to establish its toxicological profile.
Metal Halide Perovskite Single Crystals: From Growth Process to Application
Authors: Li Shuigen , Zhang Chen , Song Jiao-Jiao , Xie Xiaohu , Meng Jian-Qiao , Xu Shunjian
Published: May 2018

As a strong competitor in the field of optoelectronic applications, organic-inorganic metal hybrid perovskites have been paid much attention because of their superior characteristics, which include broad absorption from visible to near-infrared region, tunable optical and electronic properties, high charge mobility, long exciton diffusion length and carrier recombination lifetime, etc. It is noted that perovskite single crystals show remarkably low trap-state densities and long carrier diffusion lengths, which are even comparable with the best photovoltaic-quality silicon, and thus are expected to provide better optoelectronic performance. This paper reviews the recent development of crystal growth in single-, mixed-organic-cation and fully inorganic halide perovskite single crystals, in particular the solution approach. Furthermore, the application of metal hybrid perovskite single crystals and future perspectives are also highlighted.
Robust Estimation for the Single Index Model Using Pseudodistances
Authors: Toma Aida , Fulga Cristinca
Published: May 2018

For portfolios with a large number of assets, the single index model allows for expressing the large number of covariances between individual asset returns through a significantly smaller number of parameters. This avoids the constraint of having very large samples to estimate the mean and the covariance matrix of the asset returns, which practically would be unrealistic given the dynamic of market conditions. The traditional way to estimate the regression parameters in the single index model is the maximum likelihood method. Although the maximum likelihood estimators have desirable theoretical properties when the model is exactly satisfied, they may give completely erroneous results when outliers are present in the data set. In this paper, we define minimum pseudodistance estimators for the parameters of the single index model and using them we construct new robust optimal portfolios. We prove theoretical properties of the estimators, such as consistency, asymptotic normality, equivariance, robustness, and illustrate the benefits of the new portfolio optimization method for real financial data.
Factors Affecting MOOC Usage by Students in Selected Ghanaian Universities
Authors: Fianu Eli , Blewett Craig , Ampong George Oppong Appiagyei , Ofori Kwame Simpe
Published: May 2018

There has been widespread criticism about the rates of participation of students enrolled on MOOCs (Massive Open Online Courses), more importantly, the percentage of students who actively consume course materials from beginning to the end. The current study sought to investigate this trend by examining the factors that influence MOOC adoption and use by students in selected Ghanaian universities. The Unified Theory of Acceptance and Use of Technology (UTAUT) was extended to develop a research model. A survey was conducted with 270 questionnaires administered to students who had been assigned MOOCs; 204 questionnaires were retrieved for analysis. Findings of the study show that MOOC usage intention is influenced by computer self-efficacy, performance expectancy, and system quality. Results also showed that MOOC usage is influenced by facilitating conditions, instructional quality, and MOOC usage intention. Social influence and effort expectancy were found not to have a significant influence on MOOC usage intention. The authors conclude that universities must have structures and resources in place to promote the use of MOOCs by students. Computer skills training should also be part of the educational curriculum at all levels. MOOC designers must ensure that the MOOCs have good instructional quality by using the right pedagogical approaches and also ensure that the sites and learning materials are of good quality.
Dynamic Programming-Based Vessel Speed Adjustment for Energy Saving and Emission Reduction
Authors: Kim Kwang-Il , Lee Keon Myung
Published: May 2018

Maritime transportation is an economic form of mass transportation, but it is associated with significant energy consumption and pollutant emissions. External forces such as tidal currents, waves, and wind strongly influence the energy efficiency of ships. The effective management of external forces can save energy and reduce emissions. This study presents a method to build an optimal speed adjustment plan for a ship to navigate a given route. The method takes a dynamic programming (DP)-based approach to finding such an optimal plan to utilize external forces. To estimate the speed changes caused by external forces, the proposed method uses the mapping information from a combined database of ship status, marine environmental conditions, and speed changes. For the efficient manipulation of externally forced speed-change information, we used MapReduce-based operations that can handle big data and support the easy retrieval of associated data in specific situations. To evaluate the applicability of the proposed method, we applied it to real navigation situations in the southwestern sea of the Korean Peninsula. In the simulation experiments, we used real automatic identification system data and marine environmental data. The proposed method built more efficient speed adjustment plans than the fixed-speed navigation in terms of energy savings and pollutant emission reduction. The results also showed that the speed adjustment exploits external forces in a beneficial manner.
Optimal Distribution Network Reconfiguration through Integration of Cycle-Break and Genetic Algorithms
Authors: Čađenović Rade , Jakus Damir , Sarajčev Petar , Vasilj Josip
Published: May 2018

This paper presents novel cycle-break (spanning tree generation) algorithms which can be used to find the optimal distribution network topology. These algorithms (adjacency matrix/top-down/bottom-up cycle break) represent a novel way of obtaining radial network topology by cycle regrouping using adjacency matrix or elementary cycle information. Proposed methods assure connected radial network topology and can be used in combination with genetic algorithms to obtain optimal distribution network structure under minimum active power loss or network loading index framework. The cycle-break algorithms are used in initial population generation, crossover and mutation process to enhance the performance of the genetic algorithms in terms of convergence rate. These modifications make the proposed approach suitable for the use on realistic distribution networks without concern of its complexity. The algorithms are tested on a several standard test networks and the results are compared with the other existing approaches.
Renewable Energy Integrated Islanded Microgrid for Sustainable Irrigation—A Bangladesh Perspective
Authors: Shoeb Md Asaduzzaman , Shafiullah GM.
Published: May 2018

Due to high investment and maintenance costs, the government on Bangladesh is unable to provide sufficient support for grid extension and supplying electricity to remote or rural areas. The deficit in electricity introduces a crisis in powering irrigation systems, which influences negatively the country’s dominant income-generating sector, agriculture. Islanded microgrids with solar photovoltaic (PV) cells is one of the most attractive solutions for providing power to rural areas due to their cost-effectiveness, reliability and environment-friendly attributes. Therefore, a techno-economic feasibility study has been undertaken to investigate the prospects of renewable energy-based islanded microgrids to support rural electrification to power both households and irrigation systems. Three case studies based on the operation time of irrigation pumps during the day are developed using the HOMER Pro Microgrid Analysis Tool to identify the optimised configurations for the proposed system. The optimised configurations are then assessed considering the performance matrices of the cost of electricity (COE), net present cost (NPC), greenhouse gas (GHG) emissions and renewable energy fraction (RF). From the analyses, it is perceived that the operation of irrigation pumps at different times of a day is a significant influence, and the optimum method considering techno-economical evaluation is to run the irrigation pumps during the daytime by solar PV. It is evident that the proposed islanded microgrid has significant potentialities in powering irrigation systems as well as rural electrification with low energy generation costs, a contribution to the reduction of global warming and to ameliorating the energy crisis in Bangladesh in order to achieve a sustainable future.
Analysis of Integration of MEA-Based CO2 Capture and Solar Energy System for Coal-Based Power Plants Based on Thermo-Economic Structural Theory
Authors: Zhai Rongrong , Liu Hongtao , Wu Hao , Yu Hai , Yang Yongping
Published: May 2018

Installing CO2 capture plants in coal-fired power stations will reduce greenhouse gas emissions and help mitigate climate change. However, the deployment of this technology faces many obstacles—in particular, high energy consumption. Aiming to address this challenge, we investigated the integration of a solar energy system in a 1000 MW coal-fired power plant equipped with monoethanolamine (MEA)-based CO2 capture (termed PG-CC) by comparing the thermo-economic performance of two integrated systems with that of PG-CC. In the first system, solar-aided coal-fired power generation equipped with MEA-based CO2 capture (SA-PG-CC), solar thermal was used to heat the high-pressure feed water in the power plant, while the reboiler duty of the capture plant’s stripper was provided by extracted low-pressure steam from the power plant. The second system integrated the power plant with solar-aided MEA-based CO2 capture (SA-CC-PG), using solar thermal to heat the stripper’s reboiler. Both systems were simulated in EBSILON Professional and Aspen Plus and analysed using thermo-economics theory. We then evaluated each system’s thermodynamic and economic performance in terms of power generation and CO2 capture. Compared with PG-CC, the thermo-economic cost of electricity increased by 12.71% in SA-PG-CC and decreased by 9.77% in SA-CC-PG. The unit thermo-economic cost of CO2 was similar in both the PG-CC and SA-PG-CC systems, but significantly greater in SA-CC-PG. Overall, SA-PG-CC produced less power but used energy more effectively than SA-CC-PG. From a thermo-economic point of view, SA-PG-CC is therefore a better choice than SA-CC-PG.
Building Resistance and Resilience: Regeneration Should Not be Left to Chance
Authors: Long James N. , Windmuller-Campione Marcella , DeRose R. Justin
Published: May 2018

Contemporary forest planning has tasked managers with developing goals associated with resistance and resilience. In practice, silviculturists use forest structure and tree species composition to characterize goals and desired future conditions, write prescriptions, and monitor outcomes associated with resistance and resilience. Although rarely discussed in the exploding literature relating to forest resistance and resilience, silvicultural regeneration methods are important and underutilized tools to meet these goals. We propose alternative silvicultural systems for building resistance and resilience to two common large-scale bark beetle disturbance agents in the Intermountain West, United States: mountain pine beetle (Dendroctonus ponderosae Hopkins) and spruce beetle (Dendroctonus rufipennis Kirby). Shelterwood, and shelterwood-with-reserves, silvicultural systems provide the desirable facilitative characteristics of a mature overstory on maintaining advance reproduction and the establishment of new cohorts of desirable tree species. These also allow the timely regeneration of large treatment areas necessary to rapidly promote desired future conditions in the face of inevitable disturbance. When implemented proactively, regeneration treatments allow silviculturists to take advantage of currently existing vegetation for the creation of age class and tree species diversity. In general, these examples illustrate the need for proactive planning for regeneration in response to any disturbance where desired future conditions include particular species. Furthermore, we argue that timely silvicultural interventions that focus on regenerating trees may be a key factor in achieving goals relating to resilience to specific disturbance types. Waiting until after the disturbance has occurred could result in the lost opportunity to establish desired species composition or stand structure—and may well result in a considerable restoration challenge.
Achieving Perfect Coordination amongst Agents in the Co-Action Minority Game
Authors: Rajpal Hardik , Dhar Deepak
Published: May 2018

We discuss the strategy that rational agents can use to maximize their expected long-term payoff in the co-action minority game. We argue that the agents will try to get into a cyclic state, where each of the ( 2 N + 1 ) agents wins exactly N times in any continuous stretch of ( 2 N + 1 ) days. We propose and analyse a strategy for reaching such a cyclic state quickly, when any direct communication between agents is not allowed, and only the publicly available common information is the record of total number of people choosing the first restaurant in the past. We determine exactly the average time required to reach the periodic state for this strategy. We show that it varies as ( N / ln 2 ) [ 1 + α cos ( 2 π log 2 N ) ] , for large N, where the amplitude α of the leading term in the log-periodic oscillations is found be 8 π 2 ( ln 2 ) 2 exp ( − 2 π 2 / ln 2 ) ≈ 7 × 10 − 11 .
The Changing Landscape in the Genetic Etiology of Human Tooth Agenesis
Authors: Williams Meredith A. , Letra Ariadne
Published: May 2018

Despite much progress in understanding the genetics of syndromic tooth agenesis (TA), the causes of the most common, isolated TA remain elusive. Recent studies have identified novel genes and variants contributing to the etiology of TA, and revealed new pathways in which tooth development genes belong. Further, the use of new research approaches including next-generation sequencing has provided increased evidence supporting an oligogenic inheritance model for TA, and may explain the phenotypic variability of the condition. In this review, we present current knowledge about the genetic mechanisms underlying syndromic and isolated TA in humans, and highlight the value of incorporating next-generation sequencing approaches to identify causative and/or modifier genes that contribute to the etiology of TA.
The Loss of Autonomy in Abused Persons: Psychological, Moral, and Legal Dimensions
Authors: Ciurria Michelle
Published: May 2018

This paper tries to resolve a tension in popular conceptions of intimate partner violence (IPV). On the one hand, we correctly assume that all abused persons are not the same: they have irreducibly plural personalities. On the other hand, we correctly assume that abused persons suffer from a loss of autonomy. The puzzle is: if abused persons share deficits in autonomy, why does it not follow that they share a set of personality traits? I argue that the psychological states implicated in autonomy-impairment in abused persons are situation-sensitive responses to salient eliciting conditions, not personality traits. This view has substantive moral and legal implications, as it implies that abusers are responsible for inflicting severe moral harms on victim-survivors, and they may also be liable for unlawful abduction and rape, in case the abused person lives with or has sexual contact with the abuser. This is because the conditions of abuse undermine the victim-survivor’s ability to autonomously consent to cohabitation and sexual contact with the abuser. I argue that the best way of protecting people from autonomy-undermining abuse is public education.
Modeling the Population Health Impact of Introducing a Modified Risk Tobacco Product into the U.S. Market
Authors: Djurdjevic Smilja , Lee Peter N. , Weitkunat Rolf , Sponsiello-Wang Zheng , Lüdicke Frank , Baker Gizelle
Published: May 2018

Philip Morris International (PMI) has developed the Population Health Impact Model (PHIM) to quantify, in the absence of epidemiological data, the effects of marketing a candidate modified risk tobacco product (cMRTP) on the public health of a whole population. Various simulations were performed to understand the harm reduction impact on the U.S. population over a 20-year period under various scenarios. The overall reduction in smoking attributable deaths (SAD) over the 20-year period was estimated as 934,947 if smoking completely went away and between 516,944 and 780,433 if cMRTP use completely replaces smoking. The reduction in SADs was estimated as 172,458 for the World Health Organization (WHO) 2025 Target and between 70,274 and 90,155 for the gradual cMRTP uptake. Combining the scenarios (WHO 2025 Target and cMRTP uptake), the reductions were between 256,453 and 268,796, depending on the cMRTP relative exposure. These results show how a cMRTP can reduce overall population harm additionally to existing tobacco control efforts.
Do Young People Ever Sit Still? Variations in Accelerometer Counts, Muscle Activity and Heart Rate across Various Sedentary Activities in Youth
Authors: Van Ekris Evi , Chinapaw Mai J. M. , Rotteveel Joost , Altenburg Teatske M.
Published: May 2018

Evidence of adverse health effects of TV viewing is stronger than for overall sedentary behaviour in youth. One explanation may be that TV viewing involves less body movement than other sedentary activities. Variations in body movement across sedentary activities are currently unknown, as are age differences in such variations. This study examined body movement differences across various sedentary activities in children and adolescents, assessed by hip-, thigh- and wrist-worn accelerometers, muscle activity and heart rate. Body movement differences between sedentary activities and standing were also examined. Fifty-three children (aged 10–12 years) and 37 adolescents (aged 16–18 years) performed seven different sedentary activities, a standing activity, and a dancing activity (as a control activity) in a controlled setting. Each activity lasted 10 minutes. Participants wore an Actigraph on their hip and both wrists, an activPAL on their thigh and a heart rate monitor. The muscle activity of weight-bearing leg muscles was measured in a subgroup (n = 38) by surface electromyography. Variations in body movement across activities were examined using general estimation equations analysis. Children showed significantly more body movement during sedentary activities and standing than adolescents. In both age groups, screen-based sedentary activities involved less body movement than non-screen-based sedentary activities. This may explain the stronger evidence for detrimental health effects of TV viewing while evidence for child sedentary behaviour in general is inconsistent. Differences in body movement during standing and sedentary activities were relatively small. Future research should examine the potential health effects of differences in body movement between screen-based versus non-screen based and standing versus sedentary activities.
The Cholinergic Anti-Inflammatory Response and the Role of Macrophages in HIV-Induced Inflammation
Authors: Delgado-Vélez Manuel , Lasalde-Dominicci José A.
Published: May 2018

Macrophages are phagocytic immune cells that protect the body from foreign invaders and actively support the immune response by releasing anti- and proinflammatory cytokines. A seminal finding revolutionized the way macrophages are seen. The expression of the neuronal alpha7 nicotinic acetylcholine receptor (α7-nAChR) in macrophages led to the establishment of the cholinergic anti-inflammatory response (CAR) in which the activation of this receptor inactivates macrophage production of proinflammatory cytokines. This novel neuroimmune response soon began to emerge as a potential target to counteract inflammation during illness and infection states. Human immunodeficiency virus (HIV)-infected individuals suffer from chronic inflammation that persists even under antiretroviral therapy. Despite the CAR’s importance, few studies involving macrophages have been performed in the HIV field. Evidence demonstrates that monocyte-derived macrophages (MDMs) recovered from HIV-infected individuals are upregulated for α7-nAChR. Moreover, in vitro studies demonstrate that addition of an HIV viral constituent, gp120IIIB, to uninfected MDMs also upregulates the α7-nAChR. Importantly, contrary to what was expected, activation of upregulated α7-nAChRs in macrophages does not reduce inflammation, suggesting a CAR disruption. Although it is reasonable to consider this receptor as a pharmacological target, additional studies are necessary since its activity seems to differ from that observed in neurons.
PIMT/NCOA6IP Deletion in the Mouse Heart Causes Delayed Cardiomyopathy Attributable to Perturbation in Energy Metabolism
Authors: Jia Yuzhi , Liu Ning , Viswakarma Navin , Sun Ruya , Schipma Mathew J. , Shang Meng , Thorp Edward B. , Kanwar Yashpal S. , Thimmapaya Bayar , Reddy Janardan K.
Published: May 2018

PIMT/NCOA6IP, a transcriptional coactivator PRIP/NCOA6 binding protein, enhances nuclear receptor transcriptional activity. Germline disruption of PIMT results in early embryonic lethality due to impairment of development around blastocyst and uterine implantation stages. We now generated mice with Cre-mediated cardiac-specific deletion of PIMT (csPIMT−/−) in adult mice. These mice manifest enlargement of heart, with nearly 100% mortality by 7.5 months of age due to dilated cardiomyopathy. Significant reductions in the expression of genes (i) pertaining to mitochondrial respiratory chain complexes I to IV; (ii) calcium cycling cardiac muscle contraction (Atp2a1, Atp2a2, Ryr2); and (iii) nuclear receptor PPAR- regulated genes involved in glucose and fatty acid energy metabolism were found in csPIMT−/− mouse heart. Elevated levels of Nppa and Nppb mRNAs were noted in csPIMT−/− heart indicative of myocardial damage. These hearts revealed increased reparative fibrosis associated with enhanced expression of Tgfβ2 and Ctgf. Furthermore, cardiac-specific deletion of PIMT in adult mice, using tamoxifen-inducible Cre-approach (TmcsPIMT−/−), results in the development of cardiomyopathy. Thus, cumulative evidence suggests that PIMT functions in cardiac energy metabolism by interacting with nuclear receptor coactivators and this property could be useful in the management of heart failure.
Modulating Chemosensitivity of Tumors to Platinum-Based Antitumor Drugs by Transcriptional Regulation of Copper Homeostasis
Authors: Lai Yu-Hsuan , Kuo Chin , Kuo Macus Tien , Chen Helen H. W.
Published: May 2018

Platinum (Pt)-based antitumor agents have been effective in treating many human malignancies. Drug importing, intracellular shuffling, and exporting—carried out by the high-affinity copper (Cu) transporter (hCtr1), Cu chaperone (Ato x1), and Cu exporters (ATP7A and ATP7B), respectively—cumulatively contribute to the chemosensitivity of Pt drugs including cisplatin and carboplatin, but not oxaliplatin. This entire system can also handle Pt drugs via interactions between Pt and the thiol-containing amino acid residues in these proteins; the interactions are strongly influenced by cellular redox regulators such as glutathione. hCtr1 expression is induced by acute Cu deprivation, and the induction is regulated by the transcription factor specific protein 1 (Sp1) which by itself is also regulated by Cu concentration variations. Copper displaces zinc (Zn) coordination at the zinc finger (ZF) domains of Sp1 and inactivates its DNA binding, whereas Cu deprivation enhances Sp1-DNA interactions and increases Sp1 expression, which in turn upregulates hCtr1. Because of the shared transport system, chemosensitivity of Pt drugs can be modulated by targeting Cu transporters. A Cu-lowering agent (trientine) in combination with a Pt drug (carboplatin) has been used in clinical studies for overcoming Pt-resistance. Future research should aim at further developing effective Pt drug retention strategies for improving the treatment efficacy.
Fine Mapping and Candidate Gene Prediction for White Immature Fruit Skin in Cucumber (Cucumis sativus L.)
Authors: Tang Hong-Yu , Dong Xu , Wang Jian-Ke , Xia Jun-Hui , Xie Fei , Zhang Yu , Yao Xuan , Xu Yue-Jin , Wan Zheng-Jie
Published: May 2018

In this study, a single recessive gene (designated w0) was identified to control the white immature fruit color. Genetic mapping with simple sequence repeats (SSR) markers located the w0 gene in the distal region of cucumber chromosome 3 (Chr.3). Fine mapping was then conducted using the method of draft genome scaffold-assisted chromosome walking with 7304 F2 individuals, which allowed for the assignment of the gene locus to a 100.3 kb genomic DNA region with two flanking markers, Q138 and Q193. Thirteen candidate genes were predicted in the 100.3 kb region. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that the expression of the Csa3G904140 gene, which encodes a two-component response regulator-like protein, was much higher in the immature fruit skin of the green parental line (Q1) than in the white parental line (H4). A coding sequence analysis suggested that a single-base insertion occurred at the ninth exon, resulting in a frameshift mutation in Csa3G904140 of H4, and the mutation was consistent with the phenotype in 17 green/white germplasms. Therefore, Csa3G904140 was taken as the likely candidate gene controlling the immature fruit color of cultivated cucumber. This study will contribute to the cloning of candidate genes and the development of white cucumber cultivars using marker-assisted breeding.
The Making of Leukemia
Authors: González-Herrero Inés , Rodríguez-Hernández Guillermo , Luengas-Martínez Andrea , Isidro-Hernández Marta , Jiménez Rafael , García-Cenador Maria Begoña , García-Criado Francisco Javier , Sánchez-García Isidro , Vicente-Dueñas Carolina
Published: May 2018

Due to the clonal nature of human leukemia evolution, all leukemic cells carry the same leukemia-initiating genetic lesions, independently of the intrinsic tumoral cellular heterogeneity. However, the latest findings have shown that the mode of action of oncogenes is not homogeneous throughout the developmental history of leukemia. Studies on different types of hematopoietic tumors have shown that the contribution of oncogenes to leukemia is mainly mediated through the epigenetic reprogramming of the leukemia-initiating target cell. This driving of cancer by a malignant epigenetic stem cell rewiring is, however, not exclusive of the hematopoietic system, but rather represents a common tumoral mechanism that is also at work in epithelial tumors. Tumoral epigenetic reprogramming is therefore a new type of interaction between genes and their target cells, in which the action of the oncogene modifies the epigenome to prime leukemia development by establishing a new pathological tumoral cellular identity. This reprogramming may remain latent until it is triggered by either endogenous or environmental stimuli. This new view on the making of leukemia not only reveals a novel function for oncogenes, but also provides evidence for a previously unconsidered model of leukemogenesis, in which the programming of the leukemia cellular identity has already occurred at the level of stem cells, therefore showing a role for oncogenes in the timing of leukemia initiation.
Overview of Cadmium Thyroid Disrupting Effects and Mechanisms
Authors: Buha Aleksandra , Matovic Vesna , Antonijevic Biljana , Bulat Zorica , Curcic Marijana , Renieri Elisavet A. , Tsatsakis Aristidis M. , Schweitzer Amie , Wallace David
Published: May 2018

Humans are exposed to a significant number of chemicals that are suspected to produce disturbances in hormone homeostasis. Hence, in recent decades, there has been a growing interest in endocrine disruptive chemicals. One of the alleged thyroid disrupting substances is cadmium (Cd), a ubiquitous toxic metal shown to act as a thyroid disruptor and carcinogen in both animals and humans. Multiple PubMed searches with core keywords were performed to identify and evaluate appropriate studies which revealed literature suggesting evidence for the link between exposure to Cd and histological and metabolic changes in the thyroid gland. Furthermore, Cd influence on thyroid homeostasis at the peripheral level has also been hypothesized. Both in vivo and in vitro studies revealed that a Cd exposure at environmentally relevant concentrations results in biphasic Cd dose-thyroid response relationships. Development of thyroid tumors following exposure to Cd has been studied mainly using in vitro methodologies. In the thyroid, Cd has been shown to activate or stimulate the activity of various factors, leading to increased cell proliferation and a reduction in normal apoptotic activity. Evidence establishing the association between Cd and thyroid disruption remains ambiguous, with further studies needed to elucidate the issue and improve our understanding of Cd-mediated effects on the thyroid gland.
Human Chorionic Gonadotropin (hCG)—An Endocrine, Regulator of Gestation and Cancer
Authors: Heidegger Helene , Jeschke Udo
Published: May 2018

Human Chorionic Gonadotropin (hCG) is a heterodimeric glycoprotein composed of two subunits [...]
Development of New Hybrid Acrylic/Epoxy DLP-3D Printable Materials
Authors: Lantean Simone , Roppolo Ignazio , Sangermano Marco , Pirri Candido Fabrizio , Chiappone Annalisa
Published: May 2018

Light induced three dimensional (3D) printing techniques generally use printable formulations that are based on acrylic monomers because of their fast reactivity, which is balanced with their good final properties. However, the possibility to enlarge the palette of 3D printable materials is a challenging target. In this work, hybrid printable formulations that are based on acrylic and epoxy resins are presented and their printability on DLP (Digital Light Processing) machines is demonstrated. Hexanediol diacrylate (HDDA) and an epoxy resin—3,4-Epoxycylohexylmethyl-3′,4′-epoxycyxlohexane carboxylate (CE)—in different ratios are used and the influence of a bridging agent, Glycidyl methacrylate (GMA), is also investigated. The reactivity of the different active species during irradiation is evaluated and the mechanical properties, including the impact toughness, the thermo-mechanical properties, and the volumetric shrinkage, are studied on printed samples.
The Emergence of Endophytic Microbes and Their Biological Promise
Authors: Strobel Gary
Published: May 2018

As is true with animal species, plants also have an associated microflora including endophytes as well as microbes associated with the phyllosphere and rhizosphere (plant surfaces) and this is considered the plant microbiome. However, those organisms within virtually all tissues and organs of the plant are known as endophytes. Most often fungi are the most frequently recovered endophytes from plant tissues, but bacterial forms generally occur in greater numbers, but not in species varieties. The exact biological/biochemical role of the endophyte in the plant and how it interacts with the plant and other endophytes and plant associated organisms has not been intensely and carefully examined. However, this has not stopped investigators in exploring the direct utility of endophytes in boosting plant production, and discovering that endophytes can directly influence the plant to resist temperature extremes, drought, as well as the presence of disease causing organisms. Also, because of the relationships that endophytes seem to have with their host plants, they make a myriad of biologically active compounds some of which are classified as antibiotics, antioxidants, anticancer agents, volatile antimicrobial agents, immunosuppressive compounds, plant growth promoting agents, and insecticides. These endophytic compounds represent a wide range of organic molecules including terpenoids, peptides, carbohydrates, aromatics, hydrocarbons and others and it seems that these compounds may have a role in the host microbe relationship. Most recently and quite surprisingly, some endophytes have been discovered that make hydrocarbons of the types found in diesel and gasoline fuels. In addition, recently discovered are epigenetic factors relating to the biology and biochemistry of endophytes. Interestingly, only about 1–2% of the entire spectrum of 300,000 known plants have been studied for their endophyte composition. Additionally, only a few plants have ever been completely studied including all tissues for the microbes within them. Likewise, the vast majority of plants, including those in oceans and lower plant forms, have never been examined for their endophytes. Furthermore, endophytes representing the “microbiome” of world’s major food plants as they exist in their native “centers of origin” are largely unknown. This non-classical review is intended to provide background information on aspects of developments in endophyte biology and more importantly the identification of new questions in this field that need to be addressed. The review is primarily based on the author’s long held experience in this field.
What We Do Not Know about Fungal Cell Adhesion Molecules
Authors: Lipke Peter N.
Published: May 2018

There has been extensive research on structure and function of fungal cell adhesion molecules, but the most of the work has been about adhesins in Candida albicans and Saccharomyces cerevisiae. These yeasts are members of a single ascomycete order, and adhesion molecules from the six other fungal phyla are only sparsely described in the literature. In these other phyla, most of the research is at the cellular level, rather than at the molecular level, so there has been little characterization of the adhesion molecules themselves. A catalog of known adhesins shows some common features: high Ser/Thr content, tandem repeats, N- and O-glycosylations, GPI anchors, dibasic sequence motifs, and potential amyloid-forming sequences. However, none of these features is universal. Known ligands include proteins and glycans on homologous cells and host cells. Existing and novel tools can exploit the availability of genome sequences to identify and characterize new fungal adhesins. These include bioinformatics tools and well-established yeast surface display models, which could be coupled with an adhesion substrate array. Thus, new knowledge could be exploited to answer key questions in fungal ecology, animal and plant pathogenesis, and roles of biofilms in infection and biomass turnover.
Shifting Centres: Site Location and Resource Procurement on the North Coast of Cyprus over the Longue Durée of the Prehistoric Bronze Age
Authors: Webb Jennifer M.
Published: May 2018

This paper examines the relationship between site location, resource procurement, and political economy in the context of three localised centres of settlement—Vasilia, Vounous, and Lapithos—which succeeded each other in the narrow, naturally bounded north coastal strip of Cyprus during the approximately 750 years of the Early and Middle Bronze Age (ca. 2450–1700 BC). Cyprus is home to abundant copper sulphide ores and was linked to the international metal trade in the first phase of the Early Bronze Age and again in the Middle Bronze Age. In both cases, this was conducted largely, if not exclusively, via outlets on the north coast which lie close to the southern coast of Anatolia and contemporary shipping lanes but some 35–40 km distant from the nearest ore bodies in the foothills of the Troodos Mountains. Mechanisms which allowed north coast sites to overcome internal distance deterrents in order to exploit geostrategic advantages in relation to external trade include a favourable natural environment (rainfall, soils, and harbours), technological advantage, probably coercion (physical and ideological), and an ability to achieve high levels of centrality within communication and transport networks with fluctuating levels of integration and hierarchy.
Thermal–Hydrodynamic Behaviour of Coated Pivoted Pad Thrust Bearings: Comparison between Babbitt, PTFE and DLC
Authors: Katsaros Konstantinos , Bompos Dimitrios A. , Nikolakopoulos Pantelis G. , Theodossiades Stephanos
Published: May 2018

The hydrodynamic lubrication and thermal analysis of tilting pad thrust bearings has been a major subject for many studies in the field of tribology. There is only a limited number of studies regarding thrust bearings with coated surfaces. The purpose of this study is to build a parametric, iterative algorithm in order to perform a complete thermal and hydrodynamic lubrication analysis for pivoted pad thrust bearings with coatings. The analytical model is mainly based on the energy, continuity and Navier–Stokes equations, which are solved numerically with the Semi-Implicit Method for Pressure Linked Equations Consistent (SIMPLEC) method. The analysis focuses on a single pivoted pad of the thrust bearing. The thermal properties of the coating material are taken into account and the resulting thermal and flow fields are solved. The basic hydrodynamic and tribological characteristics are calculated for an uncoated, a Babbitt coated, a PTFE coated and a diamond like carbon (DLC) coated pivoted pad thrust bearing. The pressure and the film thickness distribution, as well as the load capacity and the frictional forces, are determined for several pad positions and velocities of the rotor. A mineral oil lubricant is used to estimate the shear thinning or thickening effects on the pad tribological performance. The results indicate that pads coated with PTFE and DLC show lower friction forces compared to the common steel and Babbitt applications. At the same time, the DLC coating seems to affect the bearing’s flow and thermal fields less than the PTFE, making it more suitable for thrust bearings applications.