bluuebunny/crossref_metadata_2025 huggingface dataset:
Figure S2 from IL3-Driven T Cell–Basophil Crosstalk Enhances Antitumor Immunity
Authors: Wei Jian , Mayberry Colleen L. , Lv Xiaoting , Hu Fangyan , Khan Taushif , Logan Natalie A. , Wilson John J. , Sears John D. , Chaussabel Damien , Chang Chih-Hao
Published: July 2024

IL3 supplementation effectively restrains E.G7-OVA tumor progression
Supplementary Figure 4 from PVRIG is Expressed on Stem-Like T Cells in Dendritic Cell–Rich Niches in Tumors and Its Blockade May Induce Immune Infiltration in Non-Inflamed Tumors
Authors: Alteber Zoya , Cojocaru Gady , Granit Roy Z. , Barbiro Inbal , Wool Assaf , Frenkel Masha , Novik Amit , Shuchami Adi , Liang Yu , Carmi Vered D. , Sabath Niv , Foreman Rob , Petrenko Natalia , He Jiang , Kliger Yossef , Levy-Barda Adva , Eitan Ram , Raban Oded , Sadot Eran , Sulimani Omri , Nathan Abraham Avi , Adewoye Henry , Ferre Pierre , Levine Zurit , Ophir Eran
Published: July 2024

Supplementary Figure 4
Supplementary Figure 10 from PVRIG is Expressed on Stem-Like T Cells in Dendritic Cell–Rich Niches in Tumors and Its Blockade May Induce Immune Infiltration in Non-Inflamed Tumors
Authors: Alteber Zoya , Cojocaru Gady , Granit Roy Z. , Barbiro Inbal , Wool Assaf , Frenkel Masha , Novik Amit , Shuchami Adi , Liang Yu , Carmi Vered D. , Sabath Niv , Foreman Rob , Petrenko Natalia , He Jiang , Kliger Yossef , Levy-Barda Adva , Eitan Ram , Raban Oded , Sadot Eran , Sulimani Omri , Nathan Abraham Avi , Adewoye Henry , Ferre Pierre , Levine Zurit , Ophir Eran
Published: July 2024

Supplementary Figure 10
Supplementary Table S1 from Orchestrated Codelivery of Peptide Antigen and Adjuvant to Antigen-Presenting Cells by Using an Engineered Chimeric Peptide Enhances Antitumor T-Cell Immunity
Authors: Pan Haifeng , Yu Siyuan , Zhuang Haoyun , Yang Han , Jiang Jinlu , Yang Haihui , Ren Shuling , Luo Guoxing , Yu Xuan , Chen Shuping , Lin Yanhua , Sheng Roufang , Zhang Shiyin , Yuan Quan , Huang Chenghao , Zhang Tianying , Li Tingdong , Ge Shengxiang , Zhang Jun , Xia Ningshao
Published: July 2024

Supplementary Table S1: Sequences of peptides and proteins.
Supplementary Fig. S8 from Orchestrated Codelivery of Peptide Antigen and Adjuvant to Antigen-Presenting Cells by Using an Engineered Chimeric Peptide Enhances Antitumor T-Cell Immunity
Authors: Pan Haifeng , Yu Siyuan , Zhuang Haoyun , Yang Han , Jiang Jinlu , Yang Haihui , Ren Shuling , Luo Guoxing , Yu Xuan , Chen Shuping , Lin Yanhua , Sheng Roufang , Zhang Shiyin , Yuan Quan , Huang Chenghao , Zhang Tianying , Li Tingdong , Ge Shengxiang , Zhang Jun , Xia Ningshao
Published: July 2024

Supplementary Fig. S8. Flow cytometry analysis of eTAT-mediated entry efficiency in the presence or absence of CpG.
Supplementary Fig. S7 from Orchestrated Codelivery of Peptide Antigen and Adjuvant to Antigen-Presenting Cells by Using an Engineered Chimeric Peptide Enhances Antitumor T-Cell Immunity
Authors: Pan Haifeng , Yu Siyuan , Zhuang Haoyun , Yang Han , Jiang Jinlu , Yang Haihui , Ren Shuling , Luo Guoxing , Yu Xuan , Chen Shuping , Lin Yanhua , Sheng Roufang , Zhang Shiyin , Yuan Quan , Huang Chenghao , Zhang Tianying , Li Tingdong , Ge Shengxiang , Zhang Jun , Xia Ningshao
Published: July 2024

Supplementary Fig. S7. GP33-related antigens and corresponding specific CD8 T cell responses.
Supplementary Fig. S5 from Orchestrated Codelivery of Peptide Antigen and Adjuvant to Antigen-Presenting Cells by Using an Engineered Chimeric Peptide Enhances Antitumor T-Cell Immunity
Authors: Pan Haifeng , Yu Siyuan , Zhuang Haoyun , Yang Han , Jiang Jinlu , Yang Haihui , Ren Shuling , Luo Guoxing , Yu Xuan , Chen Shuping , Lin Yanhua , Sheng Roufang , Zhang Shiyin , Yuan Quan , Huang Chenghao , Zhang Tianying , Li Tingdong , Ge Shengxiang , Zhang Jun , Xia Ningshao
Published: July 2024

Supplementary Fig. S5. Analysis of Ag uptake by APCs at the injection site.
Supplementary Fig. S3 from Orchestrated Codelivery of Peptide Antigen and Adjuvant to Antigen-Presenting Cells by Using an Engineered Chimeric Peptide Enhances Antitumor T-Cell Immunity
Authors: Pan Haifeng , Yu Siyuan , Zhuang Haoyun , Yang Han , Jiang Jinlu , Yang Haihui , Ren Shuling , Luo Guoxing , Yu Xuan , Chen Shuping , Lin Yanhua , Sheng Roufang , Zhang Shiyin , Yuan Quan , Huang Chenghao , Zhang Tianying , Li Tingdong , Ge Shengxiang , Zhang Jun , Xia Ningshao
Published: July 2024

Supplementary Fig. S3. Flow cytometric analysis of SIINFEKL-H-2Kb complex expression on BMDCs treated with OVA-related antigens.
Supplementary Fig. S22 from Orchestrated Codelivery of Peptide Antigen and Adjuvant to Antigen-Presenting Cells by Using an Engineered Chimeric Peptide Enhances Antitumor T-Cell Immunity
Authors: Pan Haifeng , Yu Siyuan , Zhuang Haoyun , Yang Han , Jiang Jinlu , Yang Haihui , Ren Shuling , Luo Guoxing , Yu Xuan , Chen Shuping , Lin Yanhua , Sheng Roufang , Zhang Shiyin , Yuan Quan , Huang Chenghao , Zhang Tianying , Li Tingdong , Ge Shengxiang , Zhang Jun , Xia Ningshao
Published: July 2024

Supplementary Fig. S22. SDS-PAGE analysis of the different purified MC38-related Ags under reducing conditions.
Supplementary Fig. S21 from Orchestrated Codelivery of Peptide Antigen and Adjuvant to Antigen-Presenting Cells by Using an Engineered Chimeric Peptide Enhances Antitumor T-Cell Immunity
Authors: Pan Haifeng , Yu Siyuan , Zhuang Haoyun , Yang Han , Jiang Jinlu , Yang Haihui , Ren Shuling , Luo Guoxing , Yu Xuan , Chen Shuping , Lin Yanhua , Sheng Roufang , Zhang Shiyin , Yuan Quan , Huang Chenghao , Zhang Tianying , Li Tingdong , Ge Shengxiang , Zhang Jun , Xia Ningshao
Published: July 2024

Supplementary Fig. S21. SDS-PAGE analysis of the different purified B16-F10-related Ags under reducing conditions.
Supplementary Fig. S2 from Orchestrated Codelivery of Peptide Antigen and Adjuvant to Antigen-Presenting Cells by Using an Engineered Chimeric Peptide Enhances Antitumor T-Cell Immunity
Authors: Pan Haifeng , Yu Siyuan , Zhuang Haoyun , Yang Han , Jiang Jinlu , Yang Haihui , Ren Shuling , Luo Guoxing , Yu Xuan , Chen Shuping , Lin Yanhua , Sheng Roufang , Zhang Shiyin , Yuan Quan , Huang Chenghao , Zhang Tianying , Li Tingdong , Ge Shengxiang , Zhang Jun , Xia Ningshao
Published: July 2024

Supplementary Fig. S2. Maturation of BMDCs following treatment with different OVA antigens.
Supplementary Fig. S18 from Orchestrated Codelivery of Peptide Antigen and Adjuvant to Antigen-Presenting Cells by Using an Engineered Chimeric Peptide Enhances Antitumor T-Cell Immunity
Authors: Pan Haifeng , Yu Siyuan , Zhuang Haoyun , Yang Han , Jiang Jinlu , Yang Haihui , Ren Shuling , Luo Guoxing , Yu Xuan , Chen Shuping , Lin Yanhua , Sheng Roufang , Zhang Shiyin , Yuan Quan , Huang Chenghao , Zhang Tianying , Li Tingdong , Ge Shengxiang , Zhang Jun , Xia Ningshao
Published: July 2024

Supplementary Fig. S18. Gating scheme for flow cytometric analysis of immune cell and tumor cell populations in tumor microenvironment.
Supplementary Fig. S1 from Orchestrated Codelivery of Peptide Antigen and Adjuvant to Antigen-Presenting Cells by Using an Engineered Chimeric Peptide Enhances Antitumor T-Cell Immunity
Authors: Pan Haifeng , Yu Siyuan , Zhuang Haoyun , Yang Han , Jiang Jinlu , Yang Haihui , Ren Shuling , Luo Guoxing , Yu Xuan , Chen Shuping , Lin Yanhua , Sheng Roufang , Zhang Shiyin , Yuan Quan , Huang Chenghao , Zhang Tianying , Li Tingdong , Ge Shengxiang , Zhang Jun , Xia Ningshao
Published: July 2024

Supplementary Fig. S1. The SDS-PAGE analysis and internalization evaluation of eTAT-GFP.
Data from Small Extracellular Vesicle piR-hsa-30937 Derived from Pancreatic Neuroendocrine Neoplasms Upregulates CD276 in Macrophages to Promote Immune Evasion
Authors: Zhong Yuan , Tian Ye , Wang Yan , Bai Jian’an , Long Qin , Yan Lijun , Gong Zhihui , Gao Wei , Tang Qiyun
Published: July 2024

Abstract
The role of PIWI-interacting RNAs (piRNA) in small extracellular vesicles (sEV) derived from pancreatic neuroendocrine neoplasms (PNEN) in the tumor microenvironment (TME) remains unexplored. We used multiplex IHC to analyze the expression of CD68, CD276 (B7H3), and CD3 on PNEN. CD276+ tumor-associated macrophages (TAM) were more abundant in tumor tissues than nontumor tissues and negatively correlated with T-cell infiltration. Serum sEV piRNA sequencing was performed to identify piRNAs enriched in patients with PNEN. We then investigated the function and mechanism of sEV piR-hsa-30937 in the cross-talk between tumor cells and macrophages in the PNEN TME. PNEN-derived sEV piR-hsa-30937 targeted PTEN to activate the AKT pathway and drive CD276 expression. CD276+ macrophages inhibited T-cell proliferation and IFNγ production. piR-hsa-30937 knockdown and anti-CD276 treatment suppressed progression and metastasis in a preclinical model of PNEN by enhancing T-cell immunity. Thus, our data show that PNEN-derived sEV piR-hsa-30937 promotes CD276 expression in macrophages through the PTEN/AKT pathway and that CD276+ TAMs suppress T-cell antitumor immunity. sEV piR-hsa-30937 and CD276 are potential therapeutic targets for immunotherapy of PNEN.
Supplementary Table 4 from Claudin-4 Modulates Autophagy via SLC1A5/LAT1 as a Mechanism to Regulate Micronuclei
Authors: Villagomez Fabian R. , Lang Julie , Rosario Fredrick J. , Nunez-Avellaneda Daniel , Webb Patricia , Neville Margaret , Woodruff Elizabeth R. , Bitler Benjamin G.
Published: July 2024

Survival statistics of claudin-4-interacting partners.
Supplementary Table 3 from Claudin-4 Modulates Autophagy via SLC1A5/LAT1 as a Mechanism to Regulate Micronuclei
Authors: Villagomez Fabian R. , Lang Julie , Rosario Fredrick J. , Nunez-Avellaneda Daniel , Webb Patricia , Neville Margaret , Woodruff Elizabeth R. , Bitler Benjamin G.
Published: July 2024

Survival statistics of claudin-4 with its interacting partners.
Supplementary Table 2 from Claudin-4 Modulates Autophagy via SLC1A5/LAT1 as a Mechanism to Regulate Micronuclei
Authors: Villagomez Fabian R. , Lang Julie , Rosario Fredrick J. , Nunez-Avellaneda Daniel , Webb Patricia , Neville Margaret , Woodruff Elizabeth R. , Bitler Benjamin G.
Published: July 2024

Metabolomics OVCA429 and OVCAR3
Supplementary Figure 4 from Claudin-4 Modulates Autophagy via SLC1A5/LAT1 as a Mechanism to Regulate Micronuclei
Authors: Villagomez Fabian R. , Lang Julie , Rosario Fredrick J. , Nunez-Avellaneda Daniel , Webb Patricia , Neville Margaret , Woodruff Elizabeth R. , Bitler Benjamin G.
Published: July 2024

Survival analysis in breast, lung, and stomach cancers.
Supplementary Figure 3 from Claudin-4 Modulates Autophagy via SLC1A5/LAT1 as a Mechanism to Regulate Micronuclei
Authors: Villagomez Fabian R. , Lang Julie , Rosario Fredrick J. , Nunez-Avellaneda Daniel , Webb Patricia , Neville Margaret , Woodruff Elizabeth R. , Bitler Benjamin G.
Published: July 2024

Metabolic enrichments
Movie 3 from Claudin-4 Modulates Autophagy via SLC1A5/LAT1 as a Mechanism to Regulate Micronuclei
Authors: Villagomez Fabian R. , Lang Julie , Rosario Fredrick J. , Nunez-Avellaneda Daniel , Webb Patricia , Neville Margaret , Woodruff Elizabeth R. , Bitler Benjamin G.
Published: July 2024

Movie 3 - mCherry
Pascal e a aposta: tradução do fragmento "Infinito Nada" e de alguns fragmentos correlatos
Authors: Loque Flavio Fontenelle
Published: July 2024

Tradução do fragmento "Infinito Nada" e de alguns fragmentos correlatos dos Pensamentos de Pascal.
Espinosa e o céu
Authors: Tatián Diego
Published: July 2024

The present text examines some of Spinoza’s references to astronomical phenomena, based on the ontological connections that various scholars and historians, from Bayle to Jacobi, have made between his thought and that of Giordano Bruno — particularly regarding the issue of pantheism. Spinoza’s thought, as well as optics, teaches that the infinite is not a harmony and that the infinite substance (the infinite power to produce infinite things in infinite modes) does not house one world below the moon and another above it, but rather only one indeterminate and open world. It proposes to conceive Spinozism as a “crossroads” between sky and earth, which does not sacrifice the science of celestial phenomena to the science of affections, nor the latter to the former.
Imagem em foco: reflexões e estudos dos processos de criação
Authors: Canjani Douglas , Cardozo Missila Loures , Spineli Patricia Kiss
Published: July 2024

Editorial do dossiê Imagem em foco: reflexões e estudos dos processos de criação. Editado por Douglas Canjani, Missila Loures Cardozo e Patricia Kiss Spineli.
Soft Skins and Rough Skins: Hundertwasser's five skins in conversation with personal poetics
Authors: Coser João Victor
Published: July 2024

This article presents an excerpt from the personal poetic production of the artist João Cóser, selected from the portfolio, and which covers five artistic works created between 2016 and 2020. The analysis of João Cóser's artistic process uses the concepts and symbologies about the skin, as constructed by the Austrian artist Hundertwasser, who defines five levels of coverings or skins for the human being: the epidermis, clothing, the home, the social environment and the global environment. The connections between the chosen poetic production and the works of other artists who deal with corporeality and the senses, such as Ernesto Neto, Chiharu Shiota and Hélio Oiticica, are also explored. The analysis also incorporates the phenomenology of the body, as developed by Merleau-Ponty and other authors who support the research. The tactile movement and manipulation of the object, whether of its visible or invisible materiality, are also addressed as prerogatives of the body.
Alencar and Lamartine, a correspondence
Authors: Pereira Patrícia Regina Cavaleiro
Published: July 2024

Transcription and translation of a letter (dated August 27, 1856) from Alphonse de Lamartine to Leonel de Alencar, Brazilian writer José de Alencar’s brother.
Collaborative Effect-Centered Problem-Solving
Authors: Hartstang Moritz , Held Jürgen
Published: July 2024

To justify the individuality of design as a discipline, researchers repeatedly separated it from other disciplines while underscoring the integration of design in those disciplines. We resolve this apparent paradox by recognizing that people have knowledge, abilities, and tasks that can be attributed to several disciplines. We emphasize a collaborative effect-centered understanding and execution of problem-solving rather than an individual and design-centered one to enable each person to perform to the best of their abilities. Therefore, we introduce the newly developed “effect method,” which, by researching defined interrelations between subjects (terms considered as single entities) and systems (terms considered as multipart entities), empowers people to identify and understand relevant problems and solution approaches. The interrelations are defined by effects to be prevented (problem) or achieved (solution). Based on this, the effect method enables the development of system-compatible solution concepts. As a result, each person's creativity, knowledge, and best abilities can contribute to the collaborative development of relevant solutions.
Phylogenetic relationships of Malesian Lady Ferns (Athyrium) provide insight into long-standing species complexes
Authors: WARDANI WITA , SALAMAH ANDI , ADJIE BAYU , YULITA KUSUMADEWI SRI , FRASER-JENKINS CHRISTOPHER ROY , WEI RAN , SUNDUE MICHAEL
Published: July 2024

We conducted a molecular phylogenetic study of Athyrium focusing on species from Java, Bali, and Sulawesi using five chloroplast markers. We sample from type localities to help resolve the application of names which have been applied inconsistently throughout the Malesian floristic area and tropical Asia. We show that A. nigripes is known with certainty only from Indonesia and perhaps Malaysia, corroborating earlier conclusions that the name A. nigripes does not apply to plants from mainland China, or India. Moreover, we show that A. nigripes is closely related to A. pulcherrimum, a narrow endemic from Mt. Pangrango, Java. We also find a large polytomy of species including A. erythropodum from both Java and Taiwan, A. auriculatum, A. brevipinnulatum, and A. mearnsianum, that helps to both clarify species concepts and raises new questions about the application of names. Finally, we show that although the presence of spines and setae do not coincide with a recent infrageneric classification of Athyrium, these characters remain useful for distinguishing large species groups.
Exploring Pico de São Tomé (São Tomé and Príncipe), a hotspot for collecting plant specimens during the colonial period: collectors and itineraries
Authors: FIGUEIREDO ESTRELA , SMITH GIDEON F. , LIMA RICARDO F. DE
Published: July 2024

The peak Pico de São Tomé on the island of São Tomé (São Tomé and Príncipe, Gulf of Guinea) is a locality where many specimens that became types of vascular plant names were collected during colonial times. Determining the routes the collectors took to ascend the peak will help determine some collecting localities. Gustav Mann was the first collector to explore the region; his itinerary is analysed and mapped based on his correspondence. The itineraries of ten further expeditions that ascended the peak are analysed. Collections that became types of vascular plant species names are recorded.
Agaricus karakensis sp. nov. (Agaricaceae, Agaricales), a new species from Pakistan
Authors: ULLAH TAUSEEF , ULLAH KHETAB , KAMAL ASIF , SABA MALKA
Published: July 2024

There are around 500 species of saprobic basidiomycetes in the genus Agaricus, of which 21 are reported from Pakistan. In this study, Agaricus karakensis sp. nov. is described as a new species from the tropical semi-arid District Karak, Khyber Pakhtunkhwa, Pakistan. The taxonomic identification was based on morphological features and ITS-region of rDNA. Both, field data and morpho-anatomical characterizations support that our specimens belong to a new species of Agaricus section Bivelares from family Agaricaceae, order Agaricales. In the phylogenetic tree, A. karakensis fall in section Bivelares forming a sister branch with A. bitorquis. In this paper, the nearest falling species were also compared with our novel finding in morphology.
Freshwater fishes of the Northeastern Mata Atlântica ecoregion, Brazil: an updated checklist with distributional patterns of a highly endemic ichthyofauna
Authors: VIEIRA-GUIMARÃES FELIPE , MARTINS-PINHEIRO RONALDO FERNANDO , SARMENTO-SOARES LUISA MARIA
Published: July 2024

We present an annotated and updated checklist of freshwater fish species occurring in the Northeastern Mata Atlântica ecoregion (NMAF), Eastern Brazil. A total of 305 native freshwater fish species are documented for this ecoregion, of which 210 are endemic. Distribution maps of 298 species are provided. There is a general pattern of increased sampling intensity in coastal zones, gradually diminishing towards the west as elevation increases. A bioregionalization algorithm identified four biogeographic units in the NMAF ecoregion: Northern Bioregion, Central Bioregion, Coastal Tablelands Bioregion and Southern Bioregion. The heterogeneous nature of the ichthyofauna in this area, along with the significant faunal overlap observed between NMAF and adjacent ecoregions, suggests that the approach of ecoregion delimitation may not be fully congruent with the current faunal compositions. This underscores the need for novel regionalization approaches that reinforce outcomes consistent with the diversity patterns evident within the ecoregion.
Graphene-based nanotechnology in the Internet of Things: a mini review
Authors: Ganguly Sharmi , Sengupta Joydip
Published: July 2024

Graphene, a 2D nanomaterial, has garnered significant attention in recent years due to its exceptional properties, offering immense potential for revolutionizing various technological applications. In the context of the Internet of Things (IoT), which demands seamless connectivity and efficient data processing, graphene's unique attributes have positioned it as a promising candidate to prevail over challenges and optimize IoT systems. This review paper aims to provide a brief sketch of the diverse applications of graphene in IoT, highlighting its contributions to sensors, communication systems, and energy storage devices. Additionally, it discusses potential challenges and prospects for the integration of graphene in the rapidly evolving IoT landscape.
Genome sequence data of the contemporary fresh-market tomatoes
Authors: Kim Juhee , Lee Tong Geon
Published: July 2024

Objective

The fresh-market tomato (Solanum lycopersicum) is bred for direct human consumption. It is selected for specific traits to meet market demands and production systems, and unique genetic variations underlying fresh-market tomato yields have been recently identified. However, DNA sequence variant-trait associations are not yet fully examined even for major traits. To provide a rich genome sequence resource for various genetics and breeding goals for fresh-market tomato traits, we report whole genome sequence data of a pool of contemporary U.S. fresh-market tomatoes.

Data description

Eighty-one tomatoes were nominated by academic tomato breeding programs in the U.S. Of the 81 tomatoes, 68 were contemporary fresh-market tomatoes, whereas the remaining 13 were relevant fresh-market tomato breeding and germplasm accessions. Whole genome sequencing (WGS) of the 81 tomatoes was conducted using the Illumina next-generation sequencing technology. The polymerase chain reaction (PCR)-free, paired-end sequencing libraries were sequenced on an average depth per sequenced base of 24 × for each tomato. This data note enhances visibility and potential for use of the more diverse, freely accessible whole genome sequence data of contemporary fresh-market tomatoes.
Genome-wide association study provided insights into the polled phenotype and polled intersex syndrome (PIS) in goats
Authors: Zhang Fuhong , Liu Qingqing , Gong Ping , Wang Yaling , Shi Chenbo , Zhu Lu , Zhao Jianqing , Yao Weiwei , Luo Jun
Published: July 2024

Background

Breeding polled goats is a welfare-friendly approach for horn removal in comparison to invasive methods. To gain a comprehensive understanding of the genetic basis underlying polledness in goats, we conducted whole-genome sequencing of 106 Xinong Saanen dairy goats, including 33 horned individuals, 70 polled individuals, and 3 polled intersexuality syndrome (PIS) individuals.

Methods

The present study employed a genome-wide association study (GWAS) and linkage disequilibrium (LD) analysis to precisely map the genetic locus underlying the polled phenotype in goats.

Results

The analysis conducted in our study revealed a total of 320 genome-wide significant single nucleotide polymorphisms (SNPs) associated with the horned/polled phenotype in goats. These SNPs exhibited two distinct peaks on chromosome 1, spanning from 128,817,052 to 133,005,441 bp and from 150,336,143 to 150,808,639 bp. The present study identified three genome-wide significant SNPs, namely Chr1:129789816, Chr1:129791507, and Chr1:129791577, as potential markers of PIS-affected goats. The results of our LD analysis suggested a potential association between MRPS22 and infertile intersex individuals, as well as a potential association between ERG and the polled trait in goats.

Conclusion

We have successfully identified three marker SNPs closely linked to PIS, as well as several candidate genes associated with the polled trait in goats. These results may contribute to the development of SNP chips for early prediction of PIS in goats, thereby facilitating breeding programs aimed at producing fertile herds with polled traits.
Genome-wide identification and expression analysis of histone deacetylase and histone acetyltransferase genes in response to drought in poplars
Authors: Li Huanhuan , Chen Yao , Dai Yujie , Yang Le , Zhang Sheng
Published: July 2024

Background

Histone deacetylases (HDACs) and histone acetyltransferases (HATs) are involved in plant growth and development as well as in response to environmental changes, by dynamically regulating gene acetylation levels. Although there have been numerous reports on the identification and function of HDAC and HAT in herbaceous plants, there are fewer report related genes in woody plants under drought stress.

Results

In this study, we performed a genome-wide analysis of the HDAC and HAT families in Populus trichocarpa, including phylogenetic analysis, gene structure, conserved domains, and expression analysis. A total of 16 PtrHDACs and 12 PtrHATs were identified in P. trichocarpa genome. Analysis of cis-elements in the promoters of PtrHDACs and PtrHATs revealed that both gene families could respond to a variety of environmental signals, including hormones and drought. Furthermore, real time quantitative PCR indicated that PtrHDA906 and PtrHAG3 were significantly responsive to drought. PtrHDA906, PtrHAC1, PtrHAC3, PtrHAG2, PtrHAG6 and PtrHAF1 consistently responded to abscisic acid, methyl jasmonate and salicylic acid under drought conditions.

Conclusions

Our study demonstrates that PtrHDACs and PtrHATs may respond to drought through hormone signaling pathways, which helps to reveal the hub of acetylation modification in hormone regulation of abiotic stress.
Pharmacotherapy and cardiovascular challenges: a case report of olverembatinib-induced myocardial infarction with non-obstructive coronary arteries
Authors: Xue Haiyan , Wang Lan , Ma Yuliang , Hou Chang
Published: July 2024

The anticancer drug of tyrosine kinase-inhibitors (TKIs) has significantly improved the prognosis of patients with specific leukemia but has also increased the risk of organ adverse reactions. Herein, we present a case of a patient diagnosed with myeloproliferative neoplasms who experienced recurrent chest pain after receiving treatment with Olverembatinib. Electrocardiography and coronary angiography confirmed the diagnosis of myocardial infarction with non-obstructive coronary arteries. This case serves as a reminder for clinicians to pay more attention and actively prevent the cardiac adverse reactions of TKIs when using such medications.
Serum miRNA-1 may serve as a promising noninvasive biomarker for predicting treatment response in breast cancer patients receiving neoadjuvant chemotherapy
Authors: Peng Jing , Lin Yanping , Sheng Xiaonan , Yuan Chenwei , Wang Yan , Yin Wenjin , Zhou Liheng , Lu Jinsong
Published: July 2024

Background

MicroRNA-1 (miR-1) is a tumour suppressor that can inhibit cell proliferation and invasion in several cancer types. In addition, miR-1 was found to be associated with drug sensitivity. Circulating miRNAs have been proven to be potential biomarkers with predictive and prognostic value. However, studies of miR-1 expression in the serum of breast cancer (BC) patients are relatively scarce, especially in patients receiving neoadjuvant chemotherapy (NAC).

Methods

Serum samples from 80 patients were collected before chemotherapy, and RT-PCR was performed to detect the serum expression of miR-1. The correlation between miR-1 expression in serum and clinicopathological factors, including pathological complete response (pCR), was analyzed by the chi-squared test and logistic regression. KEGG and GSEA analysis were also performed to determine the biological processes and signalling pathways involved.

Results

The miR-1 high group included more patients who achieved a pCR than did the miR-1 low group (p < 0.001). Higher serum miR-1 levels showed a strong correlation with decreased ER (R = 0.368, p < 0.001) and PR (R = 0.238, p = 0.033) levels. The univariate model of miR-1 for predicting pCR achieved an AUC of 0.705 according to the ROC curve. According to the interaction analysis, miR-1 interacted with Ki67 to predict the NAC response. According to the Kaplan–Meier plot, a high serum miR-1 level was related to better disease-free survival (DFS) in the NAC cohort. KEGG analysis and GSEA results indicated that miR-1 may be related to the PPAR signalling pathway and glycolysis.

Conclusions

In summary, our data suggested that miR-1 could be a potential biomarker for pCR and survival outcomes in patients with BC treated with NAC.
Diagnosis of cystic fibrosis: a high heterogeneity of symptoms and genotypes in a Brazil population
Authors: Meneses Daniela Gois , dos Santos Fábia Regina , Botelho Anne Jardim , Bispo Luciana Mota , Matos Camilla Guerra , Propheta Vynicius Goltran Sobral , Rodrigues Alexia Ferreira , Oliveira Géssica Uruga , da Silva Angela Maria , Gurgel Ricardo Queiroz
Published: July 2024

Introduction

In highly multiracial populations with inadequate newborn screening, knowledge of the various phenotypic presentations of Cystic Fibrosis (CF) can help reach an early diagnosis. This study aims to describe phenotypes and genotypes at the time of CF diagnosis in a state in the Northeast Region of Brazil.

Methods

Retrospective cross-sectional study. Clinical data were extracted from the medical records of CF patients. Clinical, laboratory, and genotypic characteristics were described for patients admitted to a tertiary referral center between 2007 and 2021.

Results

Fifty-eight (58) patients were included in the study, 53.5% of whom were diagnosed through clinical suspicion. The median age at diagnosis was 4.7 months (IQR: 1.5–14.8 months). Five patients had false-negative results in the newborn screening. Faltering growth was the most frequent clinical manifestation. Bronchiectasis and a history of pneumonia predominated in those older than ten, while thinness, underweight, and electrolyte imbalances were more frequent in children under two. Sequencing of the CFTR gene identified 27 genotypes, with at least one class I–III variant in all patients, and nine variants that are rare, previously undescribed, or have uncertain significance (619delA, T12991, K162Q, 3195del6, 1678del > T, 124del123bp, 3121–3113 A > T). The most frequent alleles were p.Phe508del, p.Gly542*, p.Arg334Trp, and p.Ser549Arg.

Conclusions

Malnutrition and electrolyte imbalances were the most frequent phenotypes for children < 2 years and were associated with genotypes including 2 class I–III variants. Rare and previously undescribed variants were identified. The p.Gly542*, p.Arg334Trp, and p.Ser549Arg alleles were among the most frequent variants in this population.
Health literacy – study protocol for LiSa cohort study
Authors: Batalha Maria João , Gabriel Tiago , Valentim Ana , Soledade Ana , Gomes Cátia , Alves Bartolomeu , dos Santos Estêvão Soares , Passadouro Rui , Dias Sara Simões
Published: July 2024

Background

Health literacy is the degree to which individuals have the ability to find, understand, and use information and services to inform health-related decisions and actions for themselves and others, whether at home, at the workplace, in the community, marketplace, healthcare sector, or the political arena. The main aim of this project is to measure health literacy in the adult population living in the municipality of Leiria over the next 10 years. As secondary objectives it is intended to characterize anxiety and depression, metabolic risk and health behaviors in the same population and over the same period.

Methods

This is a prospective cohort study that collects data on HL, anxiety and depression, health characteristics, health behavior and sociodemographic data. The study population will be composed by adults (≥ 18 years old) who are non-institutionalized and living in private households in Leiria. The random sample is stratified by gender and age groups. A face-to-face interview will be conducted with the Computer Assisted Personal Interview at baseline. Follow-up will be carried out every 2 years via telephone call. The association between independent variables and health literacy is examined by means of variance analysis with measurement repetition, and taking into consideration follow-up.

Discussion

The LiSa project is a population-based study, derived from a random sampling technique that will allow the analysis of health outcomes in a representative sample of the population of the municipality of Leiria. The LiSa study will be a valuable resource for epidemiological research, as it will provide fundamental information to improve public health policies regarding health literacy in Portugal.

Trial registration

Clinical trials: NCT05558631 (registered on 26/09/2022).
Women’s health at work: a qualitative study on women’s health issues in relation to work participation. Experiences and perspectives from female teachers and managers in Norwegian high schools
Authors: Gjellestad Marianne , Enehaug Heidi , Haraldstad Kristin , Nilsen Vegard , Helmersen Migle
Published: July 2024

Background

Public health and working life are closely related. Even though Norway is one of the world’s most equality-oriented countries, working life is still divided by gender. Women have a lower rate of participation in working life than men, they work more part-time and they have a higher sickness absence. Research has mostly focused on structural and cultural reasons for gender differences, rather than on the fact that women and men have different biology and face different health challenges. The aim of this project was to explore experienced associations between women’s health and female participation in working life.

Methods

Qualitative methods were chosen for investigating women’s experiences. We carried out in-depth interviews with 11 female high school teachers and supplemented the material with a focus group with five managers from the same organisation. The interviews were recorded and transcribed verbatim. We used the six steps of reflexive thematic analysis for consistency in the analysis process.

Results

The teachers shared a variety of experienced health issues within the field of women’s health and perceived barriers in the work environment. Four main themes were identified: (1) invisibility of women’s health at work, (2) complexity and lack of recognition of women’s health at work, (3) women’s health in work environment and (4) women’s health and role conflicts. There were few contradictions between the two informant groups. We found that health, work and total life intertwine and that complexity, lack of recognition and invisibility of women’s health appear at different levels in a mutual influence: for the women themselves, in the organisation and in society.

Conclusion

Lack of recognition and invisibility of women’s health in the work environment is suggested to influence women’s work participation. The complexity of female health is not captured by gender-neutral structures in the work environment meant to protect and promote employees’ occupational health. Recognition of women’s health in the work context can therefore contribute to a gender-equal, health-promoting and sustainable working life.
Moniezia benedeni drives the SNAP-25 expression of the enteric nerves in sheep's small intestine
Authors: Huang Zhen , Yao Wanling , He Wanhong , Pan Jing , Chai Wenzhu , Wang Baoshan , Jia Zhitao , Fan Xiping , Wang Wenhui , Zhang Wangdong
Published: July 2024

Background

The neuroimmune network plays a crucial role in regulating mucosal immune homeostasis within the digestive tract. Synaptosome-associated protein 25 (SNAP-25) is a presynaptic membrane-binding protein that activates ILC2s, initiating the host's anti-parasitic immune response.

Methods

To investigate the effect of Moniezia benedeni (M. benedeni) infection on the distribution of SNAP-25 in the sheep's small intestine, the recombinant plasmid pET-28a-SNAP-25 was constructed and expressed in BL21, yielding the recombinant protein. Then, the rabbit anti-sheep SNAP-25 polyclonal antibody was prepared and immunofluorescence staining was performed with it. The expression levels of SNAP-25 in the intestines of normal and M. benedeni-infected sheep were detected by ELISA.

Results

The results showed that the SNAP-25 recombinant protein was 29.3 KDa, the titer of the prepared immune serum reached 1:128,000. It was demonstrated that the rabbit anti-sheep SNAP-25 polyclonal antibody could bind to the natural protein of sheep SNAP-25 specifically. The expression levels of SNAP-25 in the sheep's small intestine revealed its primary presence in the muscular layer and lamina propria, particularly around nerve fibers surrounding the intestinal glands. Average expression levels in the duodenum, jejunum, and ileum were 130.32 pg/mg, 185.71 pg/mg, and 172.68 pg/mg, respectively. Under conditions of M. benedeni infection, the spatial distribution of SNAP-25-expressing nerve fibers remained consistent, but its expression level in each intestine segment was increased significantly (P < 0.05), up to 262.02 pg/mg, 276.84 pg/mg, and 326.65 pg/mg in the duodenum, jejunum, and ileum, and it was increased by 101.06%, 49.07%, and 89.16% respectively.

Conclusions

These findings suggest that M. benedeni could induce the SNAP-25 expression levels in sheep's intestinal nerves significantly. The results lay a foundation for further exploration of the molecular mechanism by which the gastrointestinal nerve-mucosal immune network perceives parasites in sheep.
Streamlining heterologous expression of top carbonic anhydrases in Escherichia coli: bioinformatic and experimental approaches
Authors: Wei Hui , Lunin Vladimir V. , Alahuhta Markus , Himmel Michael E. , Huang Shu , Bomble Yannick J. , Zhang Min
Published: July 2024

Background

Carbonic anhydrase (CA) enzymes facilitate the reversible hydration of CO2 to bicarbonate ions and protons. Identifying efficient and robust CAs and expressing them in model host cells, such as Escherichia coli, enables more efficient engineering of these enzymes for industrial CO2 capture. However, expression of CAs in E. coli is challenging due to the possible formation of insoluble protein aggregates, or inclusion bodies. This makes the production of soluble and active CA protein a prerequisite for downstream applications.

Results

In this study, we streamlined the process of CA expression by selecting seven top CA candidates and used two bioinformatic tools to predict their solubility for expression in E. coli. The prediction results place these enzymes in two categories: low and high solubility. Our expression of high solubility score CAs (namely CA5-SspCA, CA6-SazCAtrunc, CA7-PabCA and CA8-PhoCA) led to significantly higher protein yields (5 to 75 mg purified protein per liter) in flask cultures, indicating a strong correlation between the solubility prediction score and protein expression yields. Furthermore, phylogenetic tree analysis demonstrated CA class-specific clustering patterns for protein solubility and production yields. Unexpectedly, we also found that the unique N-terminal, 11-amino acid segment found after the signal sequence (not present in its homologs), was essential for CA6-SazCA activity.

Conclusions

Overall, this work demonstrated that protein solubility prediction, phylogenetic tree analysis, and experimental validation are potent tools for identifying top CA candidates and then producing soluble, active forms of these enzymes in E. coli. The comprehensive approaches we report here should be extendable to the expression of other heterogeneous proteins in E. coli.
Human peritoneal fluid exerts ovulation- and nonovulation-sourced oncogenic activities on transforming fallopian tube epithelial cells
Authors: Hsu Che-Fang , Seenan Vaishnavi , Wang Liang-Yuan , Chen Pao-Chu , Ding Dah-Ching , Chu Tang-Yuan
Published: July 2024

Secretory cells in the fallopian tube fimbria epithelium (FTE) are regarded as the main cells of origin of ovarian high-grade serous carcinoma (HGSC). Ovulation is the main cause of FTE oncogenesis, which proceeds through a sequence of TP53 mutations, chromosomal instability due to Rb/cyclin E aberration, in situ carcinoma (STIC), and metastasis to the ovary and peritoneum (metastatic HGSC). Previously, we have identified multiple oncogenic activities of the ovulatory follicular fluid (FF), which exerts the full spectrum of transforming activity on FTE cells at different stages of transformation. After ovulation, the FF is transfused into the peritoneal fluid (PF), in which the FTE constantly bathes. We wondered whether PF exerts the same spectrum of oncogenic activities as done by FF and whether these activities are derived from FF. By using a panel of FTE cell lines with p53 mutation (FT282-V), p53/CCNE1 aberrations (FT282-CCNE1), and p53/Rb aberrations plus spontaneous transformation, and peritoneal metastasis (FEXT2), we analyzed the changes of different transformation phenotypes after treating with FF and PF collected before or after ovulation. Similar to effects exhibited by FF, we found that, to a lesser extent, PF promoted anchorage-independent growth (AIG), migration, anoikis resistance, and peritoneal attachment in transforming FTE cells. The more transformed cells were typically more affected. Among the transforming activities exhibited by PF treatment, AIG, Matrigel invasion, and peritoneal attachment growth were higher with luteal-phase PF treatment than with the proliferative-phase PF treatment, suggesting an ovulation source. In contrast, changes in anoikis resistance and migration activities were similar in response to treatment with PF collected before and after ovulation, suggesting an ovulation-independent source. The overall transforming activity of luteal-phase PF was verified in an i.p. co-injection xenograft mouse model. Co-injection of Luc-FEXT2 cells with either FF or luteal-phase PF supported early peritoneal implantation, whereas co-injection with follicular-phase PF did not. This study, for the first time, demonstrates that PF from ovulating women can promote different oncogenic phenotypes in FTE cells at different stages of malignant transformation. Most of these activities, other than anoikis resistance and cell migration, are sourced from ovulation.
NIR-responsive electrospun nanofiber dressing promotes diabetic-infected wound healing with programmed combined temperature-coordinated photothermal therapy
Authors: Fu Jinlang , Wang Ding , Tang Zinan , Xu Yixin , Xie Jiajun , Chen Rong , Wang Pinkai , Zhong Qiang , Ning Yanhong , Lei Mingyuan , Mai Huaming , Li Hao , Liu Haibing , Wang Jian , Cheng Hao
Published: July 2024

Background

Diabetic wounds present significant challenges, specifically in terms of bacterial infection and delayed healing. Therefore, it is crucial to address local bacterial issues and promote accelerated wound healing. In this investigation, we utilized electrospinning to fabricate microgel/nanofiber membranes encapsulating MXene-encapsulated microgels and chitosan/gelatin polymers.

Results

The film dressing facilitates programmed photothermal therapy (PPT) and mild photothermal therapy (MPTT) under near-infrared (NIR), showcasing swift and extensive antibacterial and biofilm-disrupting capabilities. The PPT effect achieves prompt sterilization within 5 min at 52 °C and disperses mature biofilm within 10 min. Concurrently, by adjusting the NIR power to induce local mild heating (42 °C), the dressing stimulates fibroblast proliferation and migration, significantly enhancing vascularization. Moreover, in vivo experimentation successfully validates the film dressing, underscoring its immense potential in addressing the intricacies of diabetic wounds.

Conclusions

The MXene microgel-loaded nanofiber dressing employs temperature-coordinated photothermal therapy, effectively amalgamating the advantageous features of high-temperature sterilization and low-temperature promotion of wound healing. It exhibits rapid, broad-spectrum antibacterial and biofilm-disrupting capabilities, exceptional biocompatibility, and noteworthy effects on promoting cell proliferation and vascularization. These results affirm the efficacy of our nanofiber dressing, highlighting its significant potential in addressing the challenge of diabetic wounds struggling to heal due to infection.

Graphical Abstract
A 20 year experience in the management of non-tubal ectopic pregnancies in a tertiary hospital – a retrospective review
Authors: Lai Theodora Hei Tung , Ko Jennifer Ka Yee , Ng Hung Yu Ernest
Published: July 2024

Background

Non-tubal ectopic pregnancies account for < 10% of all ectopic pregnancies. Due to its rarity and wide variation in clinical practice, there is no guideline or consensus for its management. We reported our 20-year experience in the management of non-tubal ectopic pregnancies in a tertiary hospital.

Methods

This is a retrospective review of all women admitted for non-tubal ectopic pregnancies from January 2003 to December 2022 in a tertiary hospital. Women with non-tubal ectopic pregnancies diagnosed by ultrasound or operation were included for analysis.

Results

Within the study period, 180 women were diagnosed to have non-tubal ectopic pregnancies at a mean gestation of 6.8 weeks. 16.7% (30/180) were conceived via assisted reproduction. Medical treatment was the first-line management option for 81 women, of which 75 (92.1%) women received intralesional methotrexate administered under transvaginal ultrasound guidance. The success rate of intralesional methotrexate ranges from 76.5% to 92.3%. Intralesional methotrexate was successful even in cases with a positive fetal pulsation or with high human chorionic gonadotrophin levels up to 252605U/L. Twenty seven women were managed expectantly and 40 underwent surgery. Nine (11.1%), two (6.1%), and one (2.3%) women required surgery due to massive or recurrent bleeding following medical, expectant, or surgical treatment. Hysterotomy and uterine artery embolization were necessary to control bleeding in one Caesarean scar and one cervical pregnancy.

Conclusions

Intralesional methotrexate is more effective than systemic methotrexate and should be considered as first line medical treatment for non-tubal ectopic pregnancies. It has a high success rate in the management of unruptured non-tubal ectopic pregnancies even in the presence of fetal pulsations or high human chorionic gonadotrophin levels, but patients may require a prolonged period of monitoring. Close surveillance and readily available surgery were required due to the risk of heavy post-procedural intra-abdominal bleeding.
Intellectual property licensing of therapeutics during the COVID-19 crisis: lessons learnt for pandemic preparedness and response
Authors: Braimoh Tiwadayo , Burrone Esteban , Gore Charles , Vijayaraghavan Pushpa
Published: July 2024

During the COVID-19 pandemic, intellectual property licensing through bilateral agreements and the Medicines Patent Pool were used to facilitate access to new COVID-19 therapeutics in low- and middle-income countries (LMICs). The lessons learnt from the application of the model to COVID-19 could be relevant for preparedness and response to future pandemics and other health emergencies.

The speed at which affordable versions of a new product are available in LMICs is key to the realization of the potential global impact of the product. When initiated early in the research and development life cycle, licensing could facilitate rapid development of generic versions of innovative products in LMICs during a pandemic. The pre-selection of qualified manufacturers, for instance building on the existing network of generic manufacturers engaged during the COVID-19 pandemic, the sharing of know-how and the quick provision of critical inputs such as reference listed drugs (RLDs) could also result in significant time saved. It is important to find a good balance between speed and quality. Necessary quality assurance terms need to be included in licensing agreements, and the potentials of the new World Health Organization Listed Authority mechanism could be explored to promote expedited regulatory reviews and timely access to safe and quality-assured products.

The number, capacity, and geographical distribution of licensed companies and the transparency of licensing agreements have implications for the sufficiency of supply, affordability, and supply security. To foster competition and support supply security, licenses should be non-exclusive. There is also a need to put modalities in place to de-risk the development of critical pandemic therapeutics, particularly where generic product development is initiated before the innovator product is proven to be effective and approved. IP licensing and technology transfer can be effective tools to improve the diversification of manufacturing and need to be explored for regional manufacturing for accelerated access at scale in in LMICs and supply security in future pandemics.