bluuebunny/crossref_metadata_2025 huggingface dataset:
Expert opinion as priors for random effects in Bayesian prediction models: Subclinical ketosis in dairy cows as an example
Authors: Ni Haifang , Klugkist Irene , van der Drift Saskia , Jorritsma Ruurd , Hooijer Gerrit , Nielen Mirjam
Published: January 2021

Random effects regression models are routinely used for clustered data in etiological and intervention research. However, in prediction models, the random effects are either neglected or conventionally substituted with zero for new clusters after model development. In this study, we applied a Bayesian prediction modelling method to the subclinical ketosis data previously collected by Van der Drift et al. (2012). Using a dataset of 118 randomly selected Dutch dairy farms participating in a regular milk recording system, the authors proposed a prediction model with milk measures as well as available test-day information as predictors for the diagnosis of subclinical ketosis in dairy cows. While their original model included random effects to correct for the clustering, the random effect term was removed for their final prediction model. With the Bayesian prediction modelling approach, we first used non-informative priors for the random effects for model development as well as for prediction. This approach was evaluated by comparing it to the original frequentist model. In addition, herd level expert opinion was elicited from a bovine health specialist using three different scales of precision and incorporated in the prediction as informative priors for the random effects, resulting in three more Bayesian prediction models. Results showed that the Bayesian approach could naturally take the clustering structure of clusters into account by keeping the random effects in the prediction model. Expert opinion could be explicitly combined with individual level data for prediction. However in this dataset, when elicited expert opinion was incorporated, little improvement was seen at the individual level as well as at the herd level. When the prediction models were applied to the 118 herds, at the individual cow level, with the original frequentist approach we obtained a sensitivity of 82.4% and a specificity of 83.8% at the optimal cutoff, while with the three Bayesian models with elicited expert opinion, we obtained sensitivities ranged from 78.7% to 84.6% and specificities ranged from 75.0% to 83.6%. At the herd level, 30 out of 118 within herd prevalences were correctly predicted by the original frequentist approach, and 31 to 44 herds were correctly predicted by the three Bayesian models with elicited expert opinion. Further investigation in expert opinion and distributional assumption for the random effects was carried out and discussed.
The Impact of the first COVID-19 shelter-in-place announcement on social distancing, difficulty in daily activities, and levels of concern in the San Francisco Bay Area: A cross-sectional social media survey
Authors: Elser Holly , Kiang Mathew V. , John Esther M. , Simard Julia F. , Bondy Melissa , Nelson Lorene M. , Chen Wei-ting , Linos Eleni
Published: January 2021

Background

The U.S. has experienced an unprecedented number of orders to shelter in place throughout the ongoing COVID-19 pandemic. We aimed to ascertain whether social distancing; difficulty with daily activities; and levels of concern regarding COVID-19 changed after the March 16, 2020 announcement of the nation’s first shelter-in-place orders (SIPO) among individuals living in the seven affected counties in the San Francisco Bay Area.

Methods

We conducted an online, cross-sectional social media survey from March 14 –April 1, 2020. We measured changes in social distancing behavior; experienced difficulties with daily activities (i.e., access to healthcare, childcare, obtaining essential food and medications); and level of concern regarding COVID-19 after the March 16 shelter-in-place announcement in the San Francisco Bay Area versus elsewhere in the U.S.

Results

In this non-representative sample, the percentage of respondents social distancing all of the time increased following the shelter-in-place announcement in the Bay Area (9.2%, 95% CI: 6.6, 11.9) and elsewhere in the U.S. (3.4%, 95% CI: 2.0, 5.0). Respondents also reported increased difficulty obtaining hand sanitizer, medications, and in particular respondents reported increased difficulty obtaining food in the Bay Area (13.3%, 95% CI: 10.4, 16.3) and elsewhere (8.2%, 95% CI: 6.6, 9.7). We found limited evidence that level of concern regarding the COVID-19 crisis changed following the announcement.

Conclusion

This study characterizes early changes in attitudes, behaviors, and difficulties. As states and localities implement, rollback, and reinstate shelter-in-place orders, ongoing efforts to more fully examine the social, economic, and health impacts of COVID-19, especially among vulnerable populations, are urgently needed.
Effect of aerobic exercise on amyloid accumulation in preclinical Alzheimer’s: A 1-year randomized controlled trial
Authors: Vidoni Eric D. , Morris Jill K. , Watts Amber , Perry Mark , Clutton Jon , Van Sciver Angela , Kamat Ashwini S. , Mahnken Jonathan , Hunt Suzanne L. , Townley Ryan , Honea Robyn , Shaw Ashley R. , Johnson David K. , Vacek James , Burns Jeffrey M.
Published: January 2021

Background

Our goal was to investigate the role of physical exercise to protect brain health as we age, including the potential to mitigate Alzheimer’s-related pathology. We assessed the effect of 52 weeks of a supervised aerobic exercise program on amyloid accumulation, cognitive performance, and brain volume in cognitively normal older adults with elevated and sub-threshold levels of cerebral amyloid as measured by amyloid PET imaging.

Methods and findings

This 52-week randomized controlled trial compared the effects of 150 minutes per week of aerobic exercise vs. education control intervention. A total of 117 underactive older adults (mean age 72.9 [7.7]) without evidence of cognitive impairment, with elevated (n = 79) or subthreshold (n = 38) levels of cerebral amyloid were randomized, and 110 participants completed the study. Exercise was conducted with supervision and monitoring by trained exercise specialists. We conducted 18F-AV45 PET imaging of cerebral amyloid and anatomical MRI for whole brain and hippocampal volume at baseline and Week 52 follow-up to index brain health. Neuropsychological tests were conducted at baseline, Week 26, and Week 52 to assess executive function, verbal memory, and visuospatial cognitive domains. Cardiorespiratory fitness testing was performed at baseline and Week 52 to assess response to exercise. The aerobic exercise group significantly improved cardiorespiratory fitness (11% vs. 1% in the control group) but there were no differences in change measures of amyloid, brain volume, or cognitive performance compared to control.

Conclusions

Aerobic exercise was not associated with reduced amyloid accumulation in cognitively normal older adults with cerebral amyloid. In spite of strong systemic cardiorespiratory effects of the intervention, the observed lack of cognitive or brain structure benefits suggests brain benefits of exercise reported in other studies are likely to be related to non-amyloid effects.

Trial registration

NCT02000583; ClinicalTrials.gov.
Predictive equations for resting metabolic rate are not appropriate to use in Brazilian male adolescent football athletes
Authors: Oliveira Taillan M. , Penna-Franca Paula A. , Dias-Silva Christian H. , Bittencourt Victor Z. , Cahuê Fabio F. L. C. , Fonseca-Junior Sidnei J. , Pierucci Anna Paola T. R.
Published: January 2021

High accuracy in estimating energy expenditure is essential for enhancing sports performance. The resting metabolic rate (RMR), as a primary component of total energy expenditure (TEE), is commonly estimated using predictive equations. However, these references may not be applicable to adolescent athletes. The purpose of this cross-sectional study was to analyse the differences between predicted RMR in relation to energy expenditure measured by indirect calorimetry (IC) among 45 Brazilian male adolescent football athletes. Indirect calorimetry (IC) and anthropometric (bioimpedance) measurements were recorded at a single visit to the laboratory after fasting overnight. The mean age was 15.6 ± 1.14 years, body mass was 63.05 ± 7.8 kg, and height was 172 ± 7.5 cm. The RMR values predicted by equations proposed by the Food and Agriculture Organization (FAO) (United Nations), Henry and Rees (HR), Harris Benedict (HB), and Cunningham (CUN) were compared with IC RMR values, by correlation analysis. The FAO and HR predictive equations yielded different values from IC (IC: 1716.26 ± 202.58, HR: 1864.87 ± 147.78, FAO: 1854.28 ± 130.19, p = 0.001). A moderate correlation of 0.504 was found between the results of HB and IC. In the survival-agreement model, the CUN equation showed low disagreement with the IC RMR, with error values between 200 and 300 kcal/day. The results showed that HB and CUN yielded similar values as IC, with the CUN equation showing low disagreement with IC; hence, adolescent athletes should undergo evaluation with precise laboratory methods to ensure that accurate information about RMR is recorded.
Palliative and end-of-life care for people living with dementia in rural areas: A scoping review
Authors: Elliot Valerie , Morgan Debra , Kosteniuk Julie , Bayly Melanie , Froehlich Chow Amanda , Cammer Allison , O’Connell Megan E.
Published: January 2021

Background and objectives

People living with dementia deserve to experience the benefits of receiving palliative care and end-of-life services and supports, yet they often do not receive this care compared to those with other terminal diseases. People living with dementia in rural areas often face additional challenges to accessing such care. The purpose of this scoping review was to systematically review and synthesize the literature on palliative and end-of-life care for people with dementia living in rural areas, and to identify and describe key findings and gaps in the literature.

Methods

A collaborative research team approach was used in an iterative process across all stages of this review. Systematic, comprehensive searches were conducted across ten databases and eight targeted websites for relevant peer-reviewed, original research and other less formal literature, published in English, which yielded a total of 4476 results. After duplicate removal, screening, and review, 24 items were included for synthesis.

Results

All items were described and illustrated by frequency distribution, findings were grouped thematically, and five key themes emerged, including: 1) Knowledge about dementia, 2) Availability, accessibility, and utilization of palliative and end-of-life care services and supports, 3) Decision-making about care, the value of a person-centered approach and collaborative support, 4) Perspectives on artificial nutrition, hydration, and comfort care, and 5) Quality of life and death. The main gap identified was literature pertaining to rural populations, especially from locations other than the United States. The influence of rurality on relevant findings was mixed across rural-urban comparison studies, as was the effect of sex and gender across the literature.

Conclusions

Several areas were highlighted including the importance of increasing knowledge about dementia, having early conversations about advanced care and treatment options, providing a person-centered approach, and the potential for using technology to address rural access issues. These findings can be used to inform future research and policy and the development of services, supports, and strategies for rural people living with dementia. Further research is recommended.
Impact of altitude on COVID-19 infection and death in the United States: A modeling and observational study
Authors: Stephens Kenton E. , Chernyavskiy Pavel , Bruns Danielle R.
Published: January 2021

Background

COVID-19, the disease caused by SARS-CoV-2, has caused a pandemic, sparing few regions. However, limited reports suggest differing infection and death rates across geographic areas including populations that reside at higher elevations (HE). We aimed to determine if COVID-19 infection, death, and case mortality rates differed in higher versus low elevation (LE) U.S. counties.

Methods

Using publicly available geographic and COVID-19 data, we calculated per capita infection and death rates and case mortality in population density matched HE and LE U.S. counties. We also performed population-scale regression analysis to investigate the association between county elevation and COVID-19 infection rates.

Findings

Population density matching of LA (< 914m, n = 58) and HE (>2133m, n = 58) counties yielded significantly lower COVID-19 cases at HE versus LE (615 versus 905, p = 0.034). HE per capita deaths were significantly lower than LE (9.4 versus 19.5, p = 0.017). However, case mortality did not differ between HE and LE (1.78% versus 1.46%, p = 0.27). Regression analysis, adjusted for relevant covariates, demonstrated decreased COVID-19 infection rates by 12.82%, 12.01%, and 11.72% per 495m of county centroid elevation, for cases recorded over the previous 30, 90, and 120 days, respectively.

Conclusions

This population-adjusted, controlled analysis suggests that higher elevation attenuates infection and death. Ongoing work from our group aims to identify the environmental, biological, and social factors of residence at HE that impact infection, transmission, and pathogenesis of COVID-19 in an effort to harness these mechanisms for future public health and/or treatment interventions.
Physiological, genomic, and sulfur isotopic characterization of methanol metabolism by Desulfovibrio carbinolicus
Authors: Sim Min Sub , Skennerton Connor T. , Orphan Victoria J.
Published: January 2021

Methanol is often considered as a non-competitive substrate for methanogenic archaea, but an increasing number of sulfate-reducing microorganisms (SRMs) have been reported to be capable of respiring with methanol as an electron donor. A better understanding of the fate of methanol in natural or artificial anaerobic systems thus requires knowledge of the methanol dissimilation by SRMs. In this study, we describe the growth kinetics and sulfur isotope effects ofDesulfovibrio carbinolicus, a methanol-oxidizing sulfate-reducing deltaproteobacterium, together with its genome sequence and annotation.D.carbinolicuscan grow with a series of alcohols from methanol to butanol. Compared to longer-chain alcohols, however, specific growth and respiration rates decrease by several fold with methanol as an electron donor. Larger sulfur isotope fractionation accompanies slowed growth kinetics, indicating low chemical potential at terminal reductive steps of respiration. In a medium containing both ethanol and methanol,D.carbinolicusdoes not consume methanol even after the cessation of growth on ethanol. Among the two known methanol dissimilatory systems, the genome ofD.carbinolicuscontains the genes coding for alcohol dehydrogenase but lacks enzymes analogous to methanol methyltransferase. We analyzed the genomes of 52 additional species of sulfate-reducing bacteria that have been tested for methanol oxidation. There is no apparent relationship between phylogeny and methanol metabolizing capacity, but most gram-negative methanol oxidizers grow poorly, and none carry homologs for methyltransferase (mtaB). Although the amount of available data is limited, it is notable that more than half of the known gram-positive methanol oxidizers have both enzymatic systems, showing enhanced growth relative to the SRMs containing only alcohol dehydrogenase genes. Thus, physiological, genomic, and sulfur isotopic results suggest thatD.carbinolicusand close relatives have the ability to metabolize methanol but likely play a limited role in methanol degradation in most natural environments.
Effectiveness of orally-delivered double-stranded RNA on gene silencing in the stinkbug Plautia stali
Authors: Nishide Yudai , Kageyama Daisuke , Tanaka Yoshiaki , Yokoi Kakeru , Jouraku Akiya , Futahashi Ryo , Fukatsu Takema
Published: January 2021

Development of a reliable method for RNA interference (RNAi) by orally-delivered double-stranded RNA (dsRNA) is potentially promising for crop protection. Considering that RNAi efficiency considerably varies among different insect species, it is important to seek for the practical conditions under which dsRNA-mediated RNAi effectively works against each pest insect. Here we investigated RNAi efficiency in the brown-winged green stinkbug Plautia stali, which is notorious for infesting various fruits and crop plants. Microinjection of dsRNA into P. stali revealed high RNAi efficiency–injection of only 30 ng dsRNA into last-instar nymphs was sufficient to knockdown target genes as manifested by their phenotypes, and injection of 300 ng dsRNA suppressed the gene expression levels by 80% to 99.9%. Knockdown experiments by dsRNA injection showed that multicopper oxidase 2 (MCO2), vacuolar ATPase (vATPase), inhibitor of apoptosis (IAP), and vacuolar-sorting protein Snf7 are essential for survival of P. stali, as has been demonstrated in other insects. By contrast, P. stali exhibited very low RNAi efficiency when dsRNA was orally administered. When 1000 ng/μL of dsRNA solution was orally provided to first-instar nymphs, no obvious phenotypes were observed. Consistent with this, RT-qPCR showed that the gene expression levels were not affected. A higher concentration of dsRNA (5000 ng/μL) induced mortality in some cohorts, and the gene expression levels were reduced to nearly 50%. Simultaneous oral administration of dsRNA against potential RNAi blocker genes did not improve the RNAi efficiency of the target genes. In conclusion, P. stali shows high sensitivity to RNAi with injected dsRNA but, unlike the allied pest stinkbugs Halyomorpha halys and Nezara viridula, very low sensitivity to RNAi with orally-delivered dsRNA, which highlights the varied sensitivity to RNAi across different species and limits the applicability of the molecular tool for controlling this specific insect pest.
A retrospective analysis of pathogen profile, antimicrobial resistance and mortality in neonatal hospital-acquired bloodstream infections from 2009–2018 at Tygerberg Hospital, South Africa
Authors: Reddy Kessendri , Bekker Adrie , Whitelaw Andrew C. , Esterhuizen Tonya M. , Dramowski Angela
Published: January 2021

Background

Analysis of hospital-acquired bloodstream infection (HA-BSI) trends is important to monitor emerging antimicrobial resistance (AMR) threats and guide empiric antibiotic choices.

Methods

A retrospective 10-year review of neonatal HA-BSI was performed at Tygerberg Hospital’s neonatal unit in Cape Town, South Africa. Neonatal clinical and laboratory data from 2014 to 2018 (Period 2) was compared with published data from 2009 to 2013 (Period 1).

Results

The neonatal unit’s HA-BSI rate declined between periods from 3.9/1000 inpatient-days in Period 1 to 3.3/1000 inpatient-days in Period 2 (p = 0.002). Pathogen yield and blood culture contamination rate were unchanged (11.0% to 10.4%, p = 0.233; 5.1% to 5.3%, p = 0.636 respectively). Gram-negative pathogens predominated (1047/1636; 64.0%); Klebsiella species, Staphylococcus aureus, Serratia marcescens, Enterococcus species and Acinetobacter baumannii were the most frequent pathogens. Extended spectrum beta-lactamase production was observed in 319/432 (73.8%) of Klebsiella species, methicillin resistance in 171/246 (69.5%) of Staphylococcus aureus and extensive drug resistance in 115/137 (83.9%) of Acinetobacter species (2009–2018). The crude mortality rate of neonatal HA-BSI episodes increased from Period 1 to Period 2 from 139/717 (19.4%) to 179/718 (24.9%) (p = 0.014), but HA-BSI attributable mortality remained unchanged (97/139 [69.8%] vs 118/179 [65.9%], p = 0.542). The in-vitro activity of piperacillin-tazobactam and amikacin declined during Period 2 (74.6% to 61.4%; p<0.001).

Conclusion

Although HA-BSI rates declined in the neonatal unit, antimicrobial resistance rates in BSI pathogens remained high. Continuous BSI surveillance is a valuable tool to detect changes in pathogen and AMR profiles and inform empiric antibiotic recommendations for neonatal units in resource-limited settings.
Amplitude of circadian rhythms becomes weaken in the north, but there is no cline in the period of rhythm in a beetle
Authors: Abe Masato S. , Matsumura Kentarou , Yoshii Taishi , Miyatake Takahisa
Published: January 2021

Many species show rhythmicity in activity, from the timing of flowering in plants to that of foraging behavior in animals. The free-running periods and amplitude (sometimes called strength or power) of circadian rhythms are often used as indicators of biological clocks. Many reports have shown that these traits are highly geographically variable, and interestingly, they often show latitudinal or longitudinal clines. In many cases, the higher the latitude is, the longer the free-running circadian period (i.e., period of rhythm) in insects and plants. However, reports of positive correlations between latitude or longitude and circadian rhythm traits, including free-running periods, the power of the rhythm and locomotor activity, are limited to certain taxonomic groups. Therefore, we collected a cosmopolitan stored-product pest species, the red flour beetle Tribolium castaneum, in various parts of Japan and examined its rhythm traits, including the power and period of the rhythm, which were calculated from locomotor activity. The analysis revealed that the power was significantly lower for beetles collected in northern areas than southern areas in Japan. However, it is worth noting that the period of circadian rhythm did not show any clines; specifically, it did not vary among the sampling sites, despite the very large sample size (n = 1585). We discuss why these cline trends were observed in T. castaneum.
Health and social concerns about living in three communities affected by per- and polyfluoroalkyl substances (PFAS): A qualitative study in Australia
Authors: Banwell Cathy , Housen Tambri , Smurthwaite Kayla , Trevenar Susan , Walker Liz , Todd Katherine , Rosas May , Kirk Martyn
Published: January 2021

Background

Exposure to per- and polyfluoroalkyl substances (PFAS) is a public health issue globally. In Australia high concentrations of PFAS have been found in environments close to sites where Aqueous Film Forming Foams (AFFF) were historically used for firefighting activities. This has resulted in significant community concern about the potential long-term health effects of these chemicals.

Objective

We describe residents’ perceptions and experiences of PFAS in three regional Australian towns where exposure has occurred.

Methods

We conducted focus groups to generate free-flowing open discussion on PFAS in three affected communities, including some with significant numbers of First Nations Peoples. We recruited participants using a range of media outlets and postal services. Focus group transcripts were analysed thematically to identify major shared concerns using Atlas Ti.

Results

One hundred and eighty residents attended fifteen focus groups that were conducted in the three communities. They included 69 First Nations People living in three communities near the town of Katherine in the Northern Territory. Study participants were concerned about potential physical health effects of exposure to PFAS, such as cancer clusters, unexplained deaths, potential exacerbation of existing health conditions, and the future health of their children. They expressed feelings of stress and anxiety about living with uncertainty related to the possible health and the socio-economic impacts of PFAS contamination in their communities.

Conclusion

While research has concentrated on the physical health effects of PFAS, more attention needs to be given to the immediate psychosocial impacts of living in an affected community.
The application of WHO ICD-PM: Feasibility for the classification of timing and causes of perinatal deaths in a busy birth centre in a low-income country
Authors: Housseine Natasha , Snieder Anne , Binsillim Mithle , Meguid Tarek , Browne Joyce L. , Rijken Marcus J.
Published: January 2021

Objective

To assess the feasibility of the application of International Classification of Diseases-10—to perinatal mortality (ICD-PM) in a busy low-income referral hospital and determine the timing and causes of perinatal deaths, and associated maternal conditions.

Design

Prospective application of ICD-PM.

Setting

Referral hospital of Mnazi Mmoja Hospital, Zanzibar, United Republic of Tanzania.

Population

Stillbirths and neonatal deaths with a birth weight above 1000 grams born between October 16th 2017 to May 31st 2018.

Methods

Clinical information and an adapted WHO ICD-PM interactive excel-based system were used to capture and classify the deaths according to timing, causes and associated maternal complications. Descriptive analysis was performed.

Main outcome measures

Timing and causes of perinatal mortality and their associated maternal conditions.

Results

There were 661 perinatal deaths of which 248 (37.5%) were neonatal deaths and 413 (62.5%) stillbirths. Of the stillbirths, 128 (31%) occurred antepartum, 129 (31%) intrapartum and for 156 (38%) the timing was unknown. Half (n = 64/128) of the antepartum stillbirths were unexplained. Two-thirds (67%, n = 87/129) of intrapartum stillbirths followed acute intrapartum events, and 30% (39/129) were unexplained. Of the neonatal deaths, 40% died after complications of intrapartum events.

Conclusion

Problems of documentation, lack of perinatal death audits, capacity for investigations, and guidelines for the unambiguous objective assignment of timing and primary causes of death are major threats for accurate determination of timing and specific primary causes of perinatal deaths.
Dynamics of water conveying zinc oxide through divergent-convergent channels with the effect of nanoparticles shape when Joule dissipation are significant
Authors: Rashid Umair , Iqbal Azhar , Liang Haiyi , Khan Waris , Ashraf Muhammad Waqar
Published: January 2021

Aim of study

The shape effects of nanoparticles are very significant in fluid flow and heat transfer. In this paper, we discuss the effects of nanoparticles shape in nanofluid flow between divergent-convergent channels theoretically. In this present study, various shapes of nanoparticles, namely sphere, column and lamina in zinc oxide-water nanofluid are used. The effect of the magnetic field and joule dissipation are also considered.

Research methodology

The system of nonlinear partial differential equations (PDEs) is converted into ordinary differential equations (ODES). The analytical solutions are successfully obtained and compared with numerical solutions. The Homotopy perturbation method and NDsolve method are used to compare analytical and numerical results respectively.

Conclusion

The results show that the lamina shape nanoparticles have higher performance in temperature disturbance and rate of heat transfer as compared to other shapes of nanoparticles.
Investigating the microbiota of fermented fish products (Pla-ra) from different communities of northeastern Thailand
Authors: Rodpai Rutchanee , Sanpool Oranuch , Thanchomnang Tongjit , Wangwiwatsin Arporn , Sadaow Lakkhana , Phupiewkham Weeraya , Boonroumkaew Patcharaporn , Intapan Pewpan M. , Maleewong Wanchai
Published: January 2021

DNA-sequencing was performed on the V3-V4 regions of 16S rRNA genes to investigate the microbial diversity of five samples of fermented freshwater fish (pla-ra) from three provinces in northeastern Thailand. The samples had salt concentrations ranging from 7 to 10%, pH values from 4.83 to 7.15, and D-/L-lactic acid concentrations of 90 to 450 mg/l. A total of 598 operational taxonomic units were annotated at various taxonomic ranks based on the SILVA Database. The lactic-acid and halophilic generaTetragenococcus,HalanaerobiumandLactobacilluswere among the dominant taxa of bacteria. The top 20 non-redundant taxa were considered in more detail. In twopla-rasamples,Tetragenococcus muriaticuswas commonly identified.Halanaerobium fermentanswas the most abundant species in a third sample and co-dominant in another sample.Lactobacillus renniniwas dominant in thepla-rasample from Roi Et Province. Additionally, other beneficial bacteria were detected includingStaphylococcus nepalensis,Lactobacillus sakei,Lactobacillus pentosus,Weissella confusa, andBifidobacterium bifidum. Differences between samples may be due to use of different raw materials, salt concentrations, recipes, processes and fermentation periods. The microbial communities inpla-raprovide a better understanding of the production outcomes of traditional products. Further optimization of the fermentation process, for example by using dominant bacterial taxa in starter cultures, may improve processes of food fermentation, food quality and flavor control, providing useful guidelines for industrial applications.
Mapping climate discourse to climate opinion: An approach for augmenting surveys with social media to enhance understandings of climate opinion in the United States
Authors: Bennett Jackson , Rachunok Benjamin , Flage Roger , Nateghi Roshanak
Published: January 2021

Surveys are commonly used to quantify public opinions of climate change and to inform sustainability policies. However, conducting large-scale population-based surveys is often a difficult task due to time and resource constraints. This paper outlines a machine learning framework—grounded in statistical learning theory and natural language processing—to augment climate change opinion surveys with social media data. The proposed framework maps social media discourse to climate opinion surveys, allowing for discerning the regionally distinct topics and themes that contribute to climate opinions. The analysis reveals significant regional variation in the emergent social media topics associated with climate opinions. Furthermore, significant correlation is identified between social media discourse and climate attitude. However, the dependencies between topic discussion and climate opinion are not always intuitive and often require augmenting the analysis with a topic’s most frequent n-grams and most representative tweets to effectively interpret the relationship. Finally, the paper concludes with a discussion of how these results can be used in the policy framing process to quickly and effectively understand constituents’ opinions on critical issues.
Source identification of infectious diseases in networks via label ranking
Authors: Zhou Jianye , Jiang Yuewen , Huang Biqing
Published: January 2021

Background

Outbreaks of infectious diseases would cause great losses to the human society. Source identification in networks has drawn considerable interest in order to understand and control the infectious disease propagation processes. Unsatisfactory accuracy and high time complexity are major obstacles to practical applications under various real-world situations for existing source identification algorithms.

Methods

This study attempts to measure the possibility for nodes to become the infection source through label ranking. A unified Label Ranking framework for source identification with complete observation and snapshot is proposed. Firstly, a basic label ranking algorithm with complete observation of the network considering both infected and uninfected nodes is designed. Our inferred infection source node with the highest label ranking tends to have more infected nodes surrounding it, which makes it likely to be in the center of infection subgraph and far from the uninfected frontier. A two-stage algorithm for source identification via semi-supervised learning and label ranking is further proposed to address the source identification issue with snapshot.

Results

Extensive experiments are conducted on both synthetic and real-world network datasets. It turns out that the proposed label ranking algorithms are capable of identifying the propagation source under different situations fairly accurately with acceptable computational complexity without knowing the underlying model of infection propagation.

Conclusions

The effectiveness and efficiency of the label ranking algorithms proposed in this study make them be of practical value for infection source identification.
Facilitating the natural semi-drying of oily sludge by changing the form of water
Authors: Liu Yucheng , Wang Maoren , Chen Mingyan , Zhu Meng , Liao Maoqi
Published: January 2021

Reducing the water content of oily sludge is essential for the disposal of it. Despite conditioning and solid-liquid separation, the water content of oily sludge generally exceeds 65%. A large amount of this water exists in the form of emulsified and bound water, reducing the efficiency of water removal during the natural semi-drying process of oily sludge. To shorten the time required for natural semi-drying, this study applied an orthogonal test to screen an oily sludge modified material (OSM). The effect and mechanism of OSM on the natural semi-drying of oily sludge were studied using a thermal gravimetric analyzer (TGA), scanning electron microscope (SEM), surface tension measurement, and microscopic observations. The results show that when the ambient temperature exceeded 10°C, the OSM increased in mass by 8–10%, and the time required for the natural semi-drying of oily sludge was shortened from 15 days to less than 5 days. OSM can rupture the emulsion, reduce the surface tension, convert the emulsion and bound water in the oily sludge into free water, and accelerate the rate of water migration, diffusion, and natural evaporation from the inside of the oily sludge to the surface and air. The research results show that changing the form of water can speed up the drying of oily sludge, creating positive economic benefits in the process of oily sludge reduction and recycling.
Assessing the applicability of the new Global Lung Function Initiative reference values for the diffusing capacity of the lung for carbon monoxide in a large population set
Authors: Wardyn Pierre-Marie , de Broucker Virginie , Chenivesse Cécile , Sobaszek Annie , Van Bulck Richard , Perez Thierry , Edmé Jean-Louis , Hulo Sébastien
Published: January 2021

Background

The single-breath diffusing capacity of the lung for carbon monoxide (DLCO) interpretation needs the comparison of measured values to reference values. In 2017, the Global Lung Function Initiative published new reference values (GLI-2017) for DLCO, alveolar volume (VA) and transfer coefficient of the lung for carbon monoxide (KCO). We aimed to assess the applicability of GLI-2017 reference values for DLCO on a large population by comparing them to the European Community of Steel and Coal equations of 1993 (ECSC-93) widely used.

Methods

In this retrospective study, spirometric indices, total lung capacity, DLCO, VA and KCO were measured in adults classified in 5 groups (controls, asthma, chronic bronchitis, cystic fibrosis, and interstitial lung diseases (ILD)). Statistical analysis comparing the 2 equations sets were stratified by sex.

Results

4180 tests were included. GLI-2017 z-scores of the 3 DLCO indices of the controls (n = 150) are nearer to 0 (expected value in a normal population) than ECSC-93 z-scores. All groups combined, in both genders, DLCO GLI-2017 z-scores and %predicted are significantly higher than ECSC z-scores and %predicted. In the ILD group, differences between the 2 equation sets depend on the DLCO impairment severity: GLI-2017 z-scores are higher than ECSC z-scores in patients with no or “mild” decrease in DLCO, but are lower in “moderate” or “severe” decrease.

Conclusion

GLI-2017 reference values for DLCO are more suitable to our population and influence the diagnostic criteria and severity definition of several lung diseases.
Comparison of obesity and metabolic syndrome prevalence using fat mass index, body mass index and percentage body fat
Authors: Wong Joseph C. , O’Neill Sheila , Beck Belinda R. , Forwood Mark R. , Khoo Soo Keat
Published: January 2021

Background

Accurate obesity classification is important so that appropriate intervention can be instituted to modify metabolic risk factors. Commonly utilized body mass index (BMI) and percentage body fat (PBF) are influenced by lean mass whereas fat mass index (FMI) measures only body fat. This study compares the prevalence of obesity and metabolic risk factors with FMI, BMI and PBF using DXA (dual-energy x-ray absorptiometry).

Methods

489 women randomly recruited from the electoral roll were stratified into 4 age groups; 40–49, 50–59, 60–69 and 70–79 years from 2000 to 2001. Clinical data and DXA body composition were obtained. Statistical analyses were performed using Medcalc v15 (Ostend, Belgium) with significance level at p = 0.05 (two-tailed).

Results

There was higher prevalence of obesity using PBF compared to BMI and FMI (p<0.001). This difference was greater from age 50–59 (p<0.05) which may be explained by age-related lean mass loss. PBF over-classified obesity in over 35% of normal and 95% of overweight categories compared to FMI and BMI. BMI has a sensitivity of 78.9% and specificity of 98.3% for obesity using FMI as the standard. BMI under-classified obesity in the overweight category by 14.9% compared to FMI. There was no difference in diabetes, dyslipidemia, hypertension and metabolic syndrome prevalence within the BMI-obesity and FMI-obesity categories (p>0.05).

Conclusion

PBF classified more obesity than BMI and FMI because of its low pre-determined threshold. The greater difference with PBF compared to BMI and FMI from the 50–59 decade onwards can be attributed to age-related lean mass loss. BMI had the lowest sensitivity for obesity diagnosis. BMI under-classified obesity in the overweight category compared to FMI due to its inability to differentiate lean mass. However, there was no significant difference in the prevalence of metabolic risk factors between BMI and FMI-obesity categories indicating that fat location may influence metabolic dysregulation.
Impact of integrating objective structured clinical examination into academic student assessment: Large-scale experience in a French medical school
Authors: Matet Alexandre , Fournel Ludovic , Gaillard François , Amar Laurence , Arlet Jean-Benoit , Baron Stéphanie , Bats Anne-Sophie , Buffel du Vaure Celine , Charlier Caroline , De Lastours Victoire , Faye Albert , Jablon Eve , Kadlub Natacha , Leguen Julien , Lebeaux David , Malmartel Alexandre , Mirault Tristan , Planquette Benjamin , Régent Alexis , Thebault Jean-Laurent , Dinh Alexy Tran , Nuzzo Alexandre , Turc Guillaume , Friedlander Gérard , Ruszniewski Philippe , Badoual Cécile , Ranque Brigitte , Oualha Mehdi , Courbebaisse Marie
Published: January 2021

Purpose

Objective structured clinical examinations (OSCE) evaluate clinical reasoning, communication skills, and interpersonal behavior during medical education. In France, clinical training has long relied on bedside clinical practice in academic hospitals. The need for a simulated teaching environment has recently emerged, due to the increasing number of students admitted to medical schools, and the necessity of objectively evaluating practical skills. This study aimed at investigating the relationships between OSCE grades and current evaluation modalities.

Methods

Three-hundred seventy-nine 4th-year students of University-of-Paris Medical School participated to the first large-scale OSCE at this institution, consisting in three OSCE stations (OSCE#1–3). OSCE#1 and #2 focused on cardiovascular clinical skills and competence, whereas OSCE#3 focused on relational skills while providing explanations before planned cholecystectomy. We investigated correlations of OSCE grades with multiple choice (MCQ)-based written examinations and evaluations of clinical skills and behavior (during hospital traineeships); OSCE grade distribution; and the impact of integrating OSCE grades into the current evaluation in terms of student ranking.

Results

The competence-oriented OSCE#1 and OSCE#2 grades correlated only with MCQ grades (r = 0.19, P<0.001) or traineeship skill grades (r = 0.17, P = 0.001), respectively, and not with traineeship behavior grades (P>0.75). Conversely, the behavior-oriented OSCE#3 grades correlated with traineeship skill and behavior grades (r = 0.19, P<0.001, and r = 0.12, P = 0.032), but not with MCQ grades (P = 0.09). The dispersion of OSCE grades was wider than for MCQ examinations (P<0.001). When OSCE grades were integrated to the final fourth-year grade with an incremental 10%, 20% or 40% coefficient, an increasing proportion of the 379 students had a ranking variation by ±50 ranks (P<0.001). This ranking change mainly affected students among the mid-50% of ranking.

Conclusion

This large-scale French experience showed that OSCE designed to assess a combination of clinical competence and behavioral skills, increases the discriminatory capacity of current evaluations modalities in French medical schools.
Three-dimensional geometric morphometric studies of modern human occipital variation
Authors: Zhang Yameng , Schepartz Lynne A.
Published: January 2021

Objectives

To investigate three-dimensional morphological variation of the occipital bone between sexes and among populations, to determine how ancestry, sex and size account for occipital shape variation and to describe the exact forms by which the differences are expressed.

Methods

CT data for 214 modern crania of Asian, African and European ancestry were compared using 3D geometric morphometrics and multivariate statistics, including principal component analysis, Hotelling’s T2test, multivariate regression, ANOVA, and MANCOVA.

Results

Sex differences in average occipital morphology are only observed in Europeans, with males exhibiting a pronounced inion. Significant ancestral differences are observed among all samples and are shared by males and females. Asian and African crania have smaller biasterionic breadths and flatter clivus angles compared to Europeans. Asian and European crania are similar in their nuchal and occipital plane proportions, nuchal and occipital angles, and lower inion positions compared to Africans. Centroid size significantly differs between sexes and among populations. The overall allometry, while significant, explains little of the shape variation. Larger occipital bones were associated with a more curved occipital plane, a pronounced inion, a narrower biasterionic breadth, a more flexed clivus, and a lower and relatively smaller foramen magnum.

Conclusions

Although significant shape differences were observed among populations, it is not recommended to use occipital morphology in sex or population estimation as both factors explained little of the observed variance. Other factors, relating to function and the environment, are suggested to be greater contributors to occipital variation. For the same reason, it is also not recommended to use the occiput in phylogenetic studies.
High blood flow shear stress values are associated with circulating tumor cells cluster disaggregation in a multi-channel microfluidic device
Authors: Marrella Alessandra , Fedi Arianna , Varani Gabriele , Vaccari Ivan , Fato Marco , Firpo Giuseppe , Guida Patrizia , Aceto Nicola , Scaglione Silvia
Published: January 2021

Metastasis represents a dynamic succession of events involving tumor cells which disseminate through the organism via the bloodstream. Circulating tumor cells (CTCs) can flow the bloodstream as single cells or as multicellular aggregates (clusters), which present a different potential to metastasize. The effects of the bloodstream-related physical constraints, such as hemodynamic wall shear stress (WSS), on CTC clusters are still unclear. Therefore, we developed, upon theoretical and CFD modeling, a new multichannel microfluidic device able to simultaneously reproduce different WSS characterizing the human circulatory system, where to analyze the correlation between SS and CTC clusters behavior. Three physiological WSS levels (i.e. 2, 5, 20 dyn/cm2) were generated, reproducing values typical of capillaries, veins and arteries. As first validation, triple-negative breast cancer cells (MDA-MB-231) were injected as single CTCs showing that higher values of WSS are correlated with a decreased viability. Next, the SS-mediated disaggregation of CTC clusters was computationally investigated in a vessels-mimicking domain. Finally, CTC clusters were injected within the three different circuits and subjected to the three different WSS, revealing that increasing WSS levels are associated with a raising clusters disaggregation after 6 hours of circulation. These results suggest that our device may represent a valid in vitro tool to carry out systematic studies on the biological significance of blood flow mechanical forces and eventually to promote new strategies for anticancer therapy.
Towards a feminist politics of mobility: U.S. Travel and immigration memoirs
Authors: Butterfield Leah
Published: January 2021

This paper challenges longstanding cultural associations that link men to mobility and women to stability by outlining what I term a feminist politics of mobility. Bringing together four contemporary memoirs that foreground journeys, I explore how U.S. women embody and represent their mobility, as well as how movement shapes their relationships to global power structures and to norms of gender and sexuality. I draw on feminist geography, feminist and queer theory, memoir studies and mobility scholarship to read Elizabeth Gilbert’s Eat Pray Love (2006), Reyna Grande’s The Distance Between Us (2012), Daisy Hernández’s A Cup of Water Under My Bed (2014), and Cheryl Strayed’s Wild (2012). Highlighting the differences between these authors’ journeys as well as the patterns across them, I ultimately find that these memoirists model a feminist politics of mobility, wherein moving through space redistributes power to women and renegotiates social relations that have historically supported women’s subordination.
Contestations of nationhood and belonging in contemporary African women travel writing
Authors: Amimo Maureen
Published: January 2021

Contemporary African travel writing produces interesting possibilities redefining the directions of the genre. One of these promises manifests in how the crisis of nationhood and belonging impacts subjects’ navigation of sites of travel. African travel narratives by women foreground fractured intimacies encumbering journeys, especially when subjects travel «home». Such texts extensively grapple with the complexities of negotiating the personal and the collective in a bid to unravel belonging. This article examines two travelogues by African women: Leah Chishugi’s A Long Way from Paradise: Surviving the Rwandan Genocide and Noo Saro-Wiwa’s Looking for Transwonderland: Travels in Nigeria as explorations of how reading precarities of nationhood through embodied travel re-imagines private journeys as a means to tease out public anxieties of nationhood and belonging. In the process of narrating precarious journeys, African women complicate the travelogue into a political statement of belonging and its paradoxes.
Prácticas discursivas feministas: análisis de los lemas de la manifestación del 8M en Bilbao
Authors: Pando-Canteli María J. , Aurrekoetxea-Casaus Maite
Published: January 2021

La movilización por la celebración del 8 de marzo de 2019 en Bilbao recibió un apoyo sin precedentes entre las manifestaciones convocadas por las organizaciones feministas hasta la fecha. En este contexto, esta investigación se propuso como objetivo analizar los lemas de las pancartas que fueron portadas por mujeres participantes a título particular en la movilización de ese día. Partiendo de una metodología cualitativa y del análisis crítico del discurso, se analizó una muestra de 154 lemas. Los resultados pusieron de manifiesto un foco discursivo centrado en la violencia, la igualdad y la lucha que interpelaba a la sociedad, a la vez que se detectaron voces individualistas que valoraban la libre elección y el empoderamiento individual por encima de los valores colectivos de solidaridad. El análisis evidencia la falta de homogeneidad en la lucha feminista, en la que conviven voces diversas, en ocasiones divergentes e incluso en conflicto, que apuntan a formas múltiples de entender al propio feminismo como un movimiento polifónico, dinámico y fluido.