bluuebunny/crossref_metadata_2025 huggingface dataset:
Mining and characterization of the PKS–NRPS hybrid for epicoccamide A: a mannosylated tetramate derivative from Epicoccum sp. CPCC 400996
Authors: Zhang Tao , Cai Guowei , Rong Xiaoting , Xu Jingwen , Jiang Bingya , Wang Hao , Li Xinxin , Wang Lu , Zhang Ran , He Wenni , Yu Liyan
Published: November 2022

Background

Genomic analysis indicated that the genomes of ascomycetes might carry dozens of biosynthetic gene clusters (BGCs), yet many clusters have remained enigmatic. The ascomycete genus Epicoccum, belonging to the family Didymellaceae, is ubiquitous that colonizes different types of substrates and is associated with phyllosphere or decaying vegetation. Species of this genus are prolific producers of bioactive substances. The epicoccamides, as biosynthetically distinct mannosylated tetramate, were first isolated in 2003 from Epicoccum sp. In this study, using a combination of genome mining, chemical identification, genetic deletion, and bioinformatic analysis, we identified the required BGC epi responsible for epicoccamide A biosynthesis in Epicoccum sp. CPCC 400996.

Results

The unconventional biosynthetic gene cluster epi was obtained from an endophyte Epicoccum sp. CPCC 400996 through AntiSMASH-based genome mining. The cluster epi includes six putative open reading frames (epiA-epiF) altogether, in which the epiA encodes a tetramate-forming polyketide synthase and nonribosomal peptide synthetases (PKS−NRPS hybrid). Sequence alignments and bioinformatic analysis to other metabolic pathways of fungal tetramates, we proposed that the gene cluster epi could be involved in generating epicoccamides. Genetic knockout of epiA completely abolished the biosynthesis of epicoccamide A (1), thereby establishing the correlation between the BGC epi and biosynthesis of epicoccamide A. Bioinformatic adenylation domain signature analysis of EpiA and other fungal PKS-NRPSs (NRPs) indicated that the EpiA is l-alanine incorporating tetramates megasynthase. Furthermore, based on the molecular structures of epicoccamide A and deduced gene functions of the cluster epi, a hypothetic metabolic pathway for biosynthesizing compound 1 was proposed. The corresponding tetramates releasing during epicoccamide A biosynthesis was catalyzed through Dieckmann-type cyclization, in which the reductive (R) domain residing in terminal module of EpiA accomplished the conversion. These results unveiled the underlying mechanism of epicoccamides biosynthesis and these findings might provide opportunities for derivatization of epicoccamides or generation of new chemical entities.

Conclusion

Genome mining and genetic inactivation experiments unveiled a previously uncharacterized PKS − NRPS hybrid-based BGC epi responsible for the generation of epicoccamide A (1) in endophyte Epicoccum sp. CPCC 400996. In addition, based on the gene cluster data, a hypothetical biosynthetic pathway of epicoccamide A was proposed.
Coating formulation change leads to inferior performance of long-lasting insecticidal nets in Papua New Guinea
Authors: Bubun Nakei , Anetul Evodia , Koinari Melanie , Freeman Timothy W. , Karl Stephan
Published: November 2022

Background

Long-lasting insecticidal nets (LLINs) play a key role in reducing malaria transmission in endemic countries. In a previous study, the authors demonstrated a substantial decrease in the bioefficacy of LLINs for malaria prevention delivered to Papua New Guinea (PNG) between 2013 and 2019. This coincided with a rise in malaria cases in the country. The present study was aimed at determining the underlying cause of the reduced bioefficacy observed in these LLINs. The main hypothesis was that a change in the coating formulation of the respective LLIN product was responsible, and had led to significantly altered product properties and performance.

Methods

A set of PermaNet® 2.0 LLIN samples (n = 12) manufactured between 2007 and 2019 was subjected to combustion ion chromatography in order to understand the chemistry of the LLIN polymer coating formulation. In addition, World Health Organization (WHO) LLIN standard wash tests and cone bioassays were conducted to further characterize the change in product performance that occurred between 2012 and 2013.

Results

High polymer fluorine content (average 3.2 g/kg) was measured in PermaNet® 2.0 manufactured up to 2012, whereas nets which were manufactured after 2012 contained very little polymer fluorine (average 0.04 g/kg) indicating a coating formulation change from a fluorocarbon (FC)-based to a non-FC-based formulation. The coating formulation change as part of the manufacturing process thus resulted in a significant reduction in bioefficacy. In addition, the manufacturing change affected wash resistance leading to a faster reduction in 24 h mosquito mortality in the non-FC-coated product with consecutive washes.

Conclusion

A change in coating formulation of PermaNet® 2.0 resulted in reduced product performance in PNG. Post-2012 PermaNet® 2.0 LLINs should not be considered to be the same product as PermaNet® 2.0 LLINs produced prior to and in 2012. Coating formulation changes should be validated to not impact LLIN product performance.
Sex-related differences in single nucleotide polymorphisms associated with dyslipidemia in a Korean population
Authors: Lee Gyeonghee , Jeon Hye Kyung , Yoo Hae Young
Published: November 2022

Background

The prevalence of dyslipidemia has increased steadily in Korea, and the incidence of dyslipidemia differs by sex. In this study, we identified single nucleotide polymorphisms (SNPs) related to dyslipidemia in Korean cohorts through genome-wide association study (GWAS) analysis.

Methods

Genotyping was conducted to determine the genotypes of 72,298 participants and investigate genotypes for 7,079,946 SNPs. Sex, age, and BMI were set as covariates for GWAS, and significant SNPs were identified in the discovery and replication stages using logistic regression.

Results

GWAS of the entire cohort revealed a total of five significant SNPs: rs117026536 (LPL), rs651821 (APOA5), rs9804646 (APOA5), rs9926440 (CETP), and rs429358 (APOE). GWAS of the male subjects revealed a total of four significant SNPs. While rs9804646 (APOA5) and rs429358 (APOE) were significant for all the subjects, rs662799 (APOA5) and rs56156922 (CETP) were significant only for the male subjects. GWAS of the female subjects revealed two significant SNPs, rs651821 (APOA5) and rs9804646 (APOA5), both of which were significant in all the subjects.

Conclusion

This is the first study to identify sex-related differences in genetic polymorphisms in Korean populations with dyslipidemia. Further studies considering environmental variables will be needed to elucidate these sex-related genetic differences in dyslipidemia.
Intravital electrochemical nanosensor as a tool for the measurement of reactive oxygen/nitrogen species in liver diseases
Authors: Abakumova Tatiana , Vaneev Alexander , Naumenko Victor , Shokhina Arina , Belousov Vsevolod , Mikaelyan Arsen , Balysheva Kamilla , Gorelkin Peter , Erofeev Alexander , Zatsepin Timofei
Published: November 2022

Reactive oxygen/nitrogen species (ROS/RNS) are formed during normal cellular metabolism and contribute to its regulation, while many pathological processes are associated with ROS/RNS imbalances. Modern methods for measuring ROS/RNS are mainly based on the use of inducible fluorescent dyes and protein-based sensors, which have several disadvantages for in vivo use. Intravital electrochemical nanosensors can be used to quantify ROS/RNS with high sensitivity without exogenous tracers and allow dynamic ROS/RNS measurements in vivo. Here, we developed a method for quantifying total ROS/RNS levels in the liver and evaluated our setup in live mice using three common models of liver disease associated with ROS activation: acute liver injury with CCl4, partial hepatectomy (HE), and induced hepatocellular carcinoma (HCC). We have demonstrated using intravital electrochemical detection that any exposure to the peritoneum in vivo leads to an increase in total ROS/RNS levels, from a slight increase to an explosion, depending on the procedure. Analysis of the total ROS/RNS level in a partial hepatectomy model revealed oxidative stress, both in mice 24 h after HE and in sham-operated mice. We quantified dose-dependent ROS/RNS production in CCl4-induced injury with underlying neutrophil infiltration and cell death. We expect that in vivo electrochemical measurements of reactive oxygen/nitrogen species in the liver may become a routine approach that provides valuable data in research and preclinical studies.
An evaluation of postmarketing reports of hyperglycaemia associated with dolutegravir for treatment of HIV in Eswatini
Authors: Duga Alemayehu L. , Magongo Sibongile , Nhlabatsi Siphesihle , Ladwar Denis O. , Härmark Linda , Rolfes Leàn
Published: November 2022

Background

Dolutegravir (DTG) is an Integrase Strand Transfer Inhibitor (INSTI) indicated in combination with other antiretroviral agents for the treatment of HIV infection. It is available in a number of pharmaceutical preparations including the fixed-dose combination (TLD) containing tenofovir (300 mg) + lamivudine (300 mg) + dolutegravir (50 mg). In 2018, Eswatini adopted TLD as the preferred first-line HIV treatment regimen for adults and adolescents as per WHO recommendations. From March 2019 to March 2020, the National Pharmacovigilance Center (NPC) in Eswatini received 8 reports of hyperglycaemia associated with the use of DTG. This study was conducted to investigate if Eswatini NPC database included cases suggestive of causality between dolutegravir and hyperglycaemia.

Method

A qualitative synthesis of information from the Eswatini national pharmacovigilance database from March 2019 to March 2020 was conducted to investigate a casual association between hyperglycaemia and dolutegravir.

Results

All reports with dolutegravir containing regimen and suspected Adverse Event of hyperglycaemia in the period of March 2019 to March 2020 were included in the study. Seven of the reports were serious (resulted in hospitalization and one case concerned optic neuritis, leading to blindness). Two patients had a medical history of diabetes while the rest of the patients had never experienced hyperglycaemia before starting dolutegravir. For all the reports, the time to onset of hyperglycaemia ranges from 2–5 months after the initiation of DTG. None of the patients discontinued the use of DTG. All the patients were treated with oral hypoglycaemic medication. In severe cases, patients were treated with intravenous normal saline and ringer lactate as well as rapid-acting insulins. All patients are currently stable on oral hypoglycaemic drugs.

Conclusion

Cases that support causality between dolutegravir containing regimen and hyperglycaemia were found. These cases were mainly serious. Based on these findings it is recommended that healthcare professionals (HCPs) actively screen all patients for risk factors of hyperglycaemia before DTG initiation. In addition, it is important that HCPs are aware of the possible association between DTG and hyperglycaemia.
Triple tSMS system (“SHIN jiba”) for non-invasive deep brain stimulation: a validation study in healthy subjects
Authors: Shibata Sumiya , Watanabe Tatsunori , Matsumoto Takuya , Yunoki Keisuke , Horinouchi Takayuki , Kirimoto Hikari , Zhang Jianxu , Wang Hen , Wu Jinglong , Onishi Hideaki , Mima Tatsuya
Published: November 2022

Background

Transcranial static magnetic field stimulation (tSMS) using a small and strong neodymium (NdFeB) magnet can temporarily suppress brain functions below the magnet. It is a promising non-invasive brain stimulation modality because of its competitive advantages such as safety, simplicity, and low-cost. However, current tSMS is insufficient to effectively stimulate deep brain areas due to attenuation of the magnetic field with the distance from the magnet. The aim of this study was to develop a brand-new tSMS system for non-invasive deep brain stimulation.

Methods

We designed and fabricated a triple tSMS system with three cylindrical NdFeB magnets placed close to each other. We compared the strength of magnetic field produced by the triple tSMS system with that by the current tSMS. Furthermore, to confirm its function, we stimulated the primary motor area in 17 healthy subjects with the triple tSMS for 20 min and assessed the cortical excitability using the motor evoked potential (MEP) obtained by transcranial magnetic stimulation.

Results

Our triple tSMS system produced the magnetic field sufficient for neuromodulation up to 80 mm depth from the magnet surface, which was 30 mm deeper than the current tSMS system. In the stimulation experiment, the triple tSMS significantly reduced the MEP amplitude, demonstrating a successful inhibition of the M1 excitability in healthy subjects.

Conclusion

Our triple tSMS system has an ability to produce an effective magnetic field in deep areas and to modulate the brain functions. It can be used for non-invasive deep brain stimulation.
MTNet: A combined diagnosis algorithm of vessel segmentation and diabetic retinopathy for retinal images
Authors: Liu Ruochen , Gao Song , Zhang Hengsheng , Wang Simin , Zhou Lun , Liu Jiaming
Published: November 2022

Medical studies have shown that the condition of human retinal vessels may reveal the physiological structure of the relationship between age-related macular degeneration, glaucoma, atherosclerosis, cataracts, diabetic retinopathy, and other ophthalmic diseases and systemic diseases, and their abnormal changes often serve as a diagnostic basis for the severity of the condition. In this paper, we design and implement a deep learning-based algorithm for automatic segmentation of retinal vessel (CSP_UNet). It mainly adopts a U-shaped structure composed of an encoder and a decoder and utilizes a cross-stage local connectivity mechanism, attention mechanism, and multi-scale fusion, which can obtain better segmentation results with limited data set capacity. The experimental results show that compared with several existing classical algorithms, the proposed algorithm has the highest blood vessel intersection ratio on the dataset composed of four retinal fundus images, reaching 0.6674. Then, based on the CSP_UNet and introducing hard parameter sharing in multi-task learning, we innovatively propose a combined diagnosis algorithm vessel segmentation and diabetic retinopathy for retinal images (MTNet). The experiments show that the diagnostic accuracy of the MTNet algorithm is higher than that of the single task, with 0.4% higher vessel segmentation IoU and 5.2% higher diagnostic accuracy of diabetic retinopathy classification.
The Slow Train to Reforming Anti-Dumping Measures
Authors: Yilmazcan Abdulkadir
Published: November 2022

This essay examines the need for and slow progress towards a revision of the Anti-Dumping Agreement. There are ongoing negotiations on the Anti-Dumping Agreement, but they are without positive outcomes. Several reasons account for this failure such as the deadlock in the Doha Development Round, mega trade agreements and the unwillingness of top anti-dumping users to engage in meaningful reform. In this paper, alternative solutions are proposed to settle the hidden trade protectionism in anti-dumping investigations. Normative solutions include a comprehensive reform of the Anti-Dumping Agreement. Such a revision has already been suggested in the literature, but this study departs from most others by prioritizing procedural issues rather than substantive ones. The study proposes changes to enhancing procedural justice in anti-dumping processes.

Keywords: World Trade Organization; Anti-Dumping Agreement; Negotiating Group on Rules.
SISTEMA DE INFORMAÇÕES GEOGRÁFICAS (SIG) COMO SUPORTE NA ELABORAÇÃO DE PLANOS DIRETORES MUNICIPAIS
Authors: Coelho André Luiz Nascentes
Published: November 2022

O presente artigo tem como objetivo apresentar os principais conceitos, fundamentos e aplicações do SIG (Sistema de Informações Geográficas) como instrumento de apóio na elaboração de Planos Diretores Municipais, abordando uma das suas etapas, que trata da análise territorial, seleção e espacialização das áreas de interesse ambiental para preservação no município da Serra, Região Metropolitana de Vitória, no estado do Espírito Santo, revelando-se uma tecnologia de suporte eficiente e de qualidade no planejamento e gestão do território.
CARTOGRAFIA GEOTÉCNICA: ESTÁGIO ATUAL DO CONHECIMENTO
Authors: Franco Gustavo Barreto , Marques Eduardo Antonio Gomes , Calijuri Maria Lucia , Gomes Ronaldo Lima
Published: November 2022

A cartografia geotécnica, nos últimos anos, tem tornado-se uma ferramenta importante para profissionais que atuam em temas relacionados ao planejamento urbano e regional. Com o objetivo de divulgar e reconhecer a potencialidade de utilização da cartografia geotécnica digital, por diferentes áreas do conhecimento, realizou-se uma revisão bibliográfica referente à sua origem, conceito, utilização, e por fim as características de um mapeamento geotécnico e as principais metodologias empregadas.
OS SOLOS DA BACIA DO RIBEIRÃO BOM JARDIM E SUAS RELAÇÕES COM RELEVO E OS RECURSOS HÍDRICOS, UBERLÂNDIA (MG)
Authors: Brito Jorge Luís Silva
Published: November 2022

The present work describes the methodology used to map the soils in the Basin of the Bom Jardim Creek (in the scale of 1:50,000) as well as the relations among the soils, relief and the hydric resources. The Bom Jardim creek is the main affluent to the Uberabinha river on its left margin. It drains an area of 396.54 km2 of lands located in the municipalities of both Uberaba and Uberlândia. The soils in the basin of the Bom Jardim creek are latosoils in the most, with medium texture, dystrophic, with low levels of organic substances. Around 20% of the basin have soils affected by hydromorphism (Neosoils, Gleisoils and Organosoils). These are found in the lane and meadow areas where the resurgence of the water table occurs. Such soils play a vital role in the hydrologic balance of the courses in the basin of the Bom Jardim creek.
MODERNIZAÇÃO AGRÍCOLA NO CERRADO MINEIRO: OS PROGRAMAS GOVERNAMENTAIS DA DÉCADA DE 1970
Authors: Gobbi Wanderléia Aparecida de Oliveira
Published: November 2022

O processo de modernização da agricultura, desencadeado na década de 1970, gerou uma grande transformação no espaço agrário brasileiro. A ação desse processo contou com a participação fundamental do Estado, via projetos governamentais. No cerrado mineiro essa realidade foi caracterizada por quatro programas, sendo dois estaduais (PCI/PADAP) e dois federais (POLOCENTRO/PRODECER), responsáveis pela capitalização da agricultura nessa região.
REFLEXÕES SOBRE O ESPAÇO GEOGRÁFICO A PARTIR DA FENOMENOLOGIA
Authors: Duarte Matusalém de Brito , Matias Vandeir Robson da Silva
Published: November 2022

O conhecimento, que significa organizar, estruturar e explicar as experiências do sujeito com o mundo dos objetos, corresponde a uma construção social da realidade e da história, adquirida pela experimentação na percepção do mundo. A construção do conhecimento acontece na observação e experimentação do sujeito espacializado, a partir dos processos políticos, econômicos, culturais e sociais que se apresentam. O espaço é objeto de estudo de vários pesquisadores: matemáticos, filósofos, geógrafos, psicólogos, sociólogos, pela sua íntima relação com à percepção e com a representação. Diante desta complexidade, é propósito deste artigo refletir sobre essa categoria, a partir da corrente fenomenológica, como aprofundamento da compreensão teórica em torno dessa categoria chave da Geografia.
CONTRADIÇÕES DE UMA “CIDADE CIENTÍFICA”: PROCESSO DE URBANIZAÇÃO E ESPECIALIZAÇÃO TERRITORIAL EM VIÇOSA (MG)
Authors: Pereira Mirlei Fachini Vicente
Published: November 2022

O artigo discute o processo de urbanização em Viçosa-MG, dando ênfase aos eventos mais dinâmicos de transformação do meio urbano e especialização científica da cidade. Destaca-se a existência de duas distintas temporalidades no lugar - um "tempo rápido", resultante da produção e especialização científica; e um "tempo lento", que caracteriza as demais relações sociais de reprodução.
Cladonia Verticillaris (Raddi) Fr., PARA DIAGNOSTICO DA SALUBRIDADE DO AR DECORRENTE DA EXTRAÇÃO E BENEFICIAMENTO DE CALCÁRIO EM VERTENTE DO LÉRIO, PERNAMBUCO (BRASIL)
Authors: Cunha Maria Helena Alves da , Silva Joelmir Marques da , Mota Filho Fernando de Oliveira , Silva Nicácio Henrique da , Pereira Eugênia Cristina Gonçalves
Published: November 2022

Os liquens têm sido utilizados como biomonitores em vários países pela sua eficiência na detecção e quantificação de poluentes atmosféricos. Neste trabalho utilizou-se Cladonia verticillaris (Raddi) Fr., com o objetivo de avaliar as alterações fisiológicas existentes após sua exposição permanente de material particulado, resultante de atividades de extração e beneficiamento de calcário em Vertente do Lério, Pernambuco (Brasil). Foram transplantadas amostras de C. verticillaris de Saloá - PE, cujo índice de poluição é quase nulo, para a área de estudo. C. verticillaris foi exposta ao ambiente no período de maio a dezembro de 2006, tendo sido recolhidas amostras mensais para ensaios. Estas foram analisadas através das Cromatografias de Camada Delgada (CCD) e a Líquida de Alta Eficiência (CLAE). Os resultados evidenciaram variações no metabolismo da espécie, que alterou a produção de sua substância principal, o ácido fumarprotocetrarico, além do acúmulo de produtos intermediários da sua biossíntese. Os efeitos foram mais evidentes nas amostras colocadas mais próximas aos emitentes, e/ou nas que estavam em localização a favor da direção predominante dos ventos.

Palavras-chave - biomonitor; liquens;poluição atmosférica; calcário.
EFEITOS DO EL NIÑO DE 1997/1998 NA PRODUÇÃO AGROPECUÁRIA NA BACIA DO RIO SUCURU - CARIRI PARAIBANO
Authors: Alencar Maria Leide Silva de , Barbosa Marx Prestes , Sousa Ridelson Farias de
Published: November 2022

O mega evento El Niño ocorrido em 1997/98, considerado o mais forte do século passado, desencadeou adversidades climáticas (secas, enchentes, temperaturas altas) em diversas regiões continentais ao redor do globo. As principais consequências de eventos como este no semi-árido estão relacionadas às secas, originando principalmente, baixa produtividade na agricultura, pecuária e reduções drásticas no abastecimento d'água aos centros urbanos e comunidades rurais. Durante muitos anos a agricultura nordestina vem passando por uma profunda crise, fundamentalmente devido à periodicidade das secas que desestimulam o plantio em sistema de sequeiro. Diante deste quadro essa pesquisa teve como objetivo avaliar a influência do evento El Niño na produção agropecuária em parte do cariri paraibano nos anos de 1997/1998 baseado em dados climatológicos, dados censitários e pesquisa de campo, onde ficou constatada uma queda considerável nos níveis de produção nos rebanhos mais relevantes, bem como nas culturas mais exploradas (milho e feijão) tanto do ponto de vista econômico como de segurança alimentar das famílias e rebanhos, para o período avaliado.
AS ALTERNATIVAS PARA O GERENCIAMENTO DOS RESÍDUOS SÓLIDOS EM CURITIBA - PR, E SUA CONTRIBUIÇÃO COM A MELHORIA DA QUALIDADE
Authors: Oliveira Nilza Aparecida da Silva
Published: November 2022

O referido artigo trata exclusivamente dos problemas relacionados à questão dos resíduos sólidos, em termos de Brasil, mas enfocando a situação de como acontece o gerenciamento desses resíduos em Curitiba Paraná, bem como destacando as alternativas encontradas pelo poder público para amenizar a questão. O objetivo principal do trabalho, consiste em mostrar que apesar de todas as dificuldades encontradas nas grandes cidades, relacionadas a esta questão, ainda é possível amenizar a situação, bastando para isso que o Poder Público e a população despertem uma consciência crítica e determine medidas que possam amenizar esta realidade. O trabalho foi realizado a partir de pesquisas de cunho teórico, tendo como base os órgãos públicos de Curitiba e do Brasil, como: Instituto Brasileiro de Geografia e Estatísticas, Instituto de Pesquisa e Planejamento Urbano de Curitiba, Compromisso empresarial para a Reciclagem e a Secretaria Estadual de Meio Ambiente. Acredita-se que a partir do momento que cada instituição partilhar as experiências que deram certo, muitas coisas boas poderão ocorrer para a melhoria de vida das pessoas do meio onde vivemos.
LEVANTAMENTO BIOGEOGRÁFICO DE MOLUSCOS NO ESTADO DE SANTA CATARINA, SC, REGIÃO SUL DO BRASIL, VERTENTE ATLÂNTICA DO CONE MERIDIONAL DA AMÉRICA DO SUL
Authors: Agudo-Padrón Aisur Ignacio
Published: November 2022

Síntese de projeto biogeográfico que completa 12 anos de exaustivo trabalho de pesquisa regional visando o levantamento integral de malacofauna marinha, terrícola e de água doce ocorrente no Estado de Santa Catarina, SC, região Sul do Brasil, apresentando razoável balanço com total de 766 espécies e subespécies confirmadas, envolvendo importantes abordagens nos terrenos da taxonomia e a ecologia, assim como exaustiva análise da produção histórica referencial regional gerada ao largo de 59 anos de produção bibliográfica, além de comparar os resultados previamente obtidos no terreno quanto ao número de espécies terrícolas e de água doce conhecidas para os restantes Estados da região Sul do Brasil (RS e PR) e os vizinhos países do Uruguai e a Argentina, geograficamente integrantes no seu conjunto da Vertente Atlântica do Cone Meridional da América do Sul.

Synthesis of biogeographical project that completes 12 years of exhaustive work of regional research seeking the integral rising of marine, terrestrial and freshwater mollusks ocurrence in the Santa Catarina's State, SC, Brazilian Southern region, presenting reasonable swinging with total of 766 species and confirmed subspecieses, involving important comments in the lands of the taxonomy and ecology, as well as exhaustive analysis of the regional bibliographical historical production generated to the 59 year-old plaza, besides to compare the results previously obtained in the land with relationship to the number of terrestrial and freshwater species acquaintances for remaining States of the brazilian Southern region (RS and PR) and the neighboring countries of Uruguay and Argentina, geographically integral in its group of the Atlantic Slope of the Southern Cone of South America.
A PRODUÇÃO DO ESPAÇO URBANO DE UBERLÂNDIA E AS POLÍTICAS PÚBLICAS DE PLANEJAMENTO
Authors: Cleps Geisa Daise Gumiero
Published: November 2022

Evidencia-se hoje que mais da metade da população mundial vive exclusivamente nas cidades, promovendo cenários de lutas e interesses urbanos diferenciados e em diversas escalas. Nesse contexto, o planejamento e a gestão urbana tornaram-se elementos essenciais ao processo de governança. No Brasil, com o crescente aumento dos problemas urbanos, onde mais de 80% da população vive em áreas urbanas, o planejamento urbano tornou-se uma estratégia que objetiva proporcionar melhores condições de vida à população, principalmente sob a ótica da justiça social. Por isso tem sido praticado não somente pelo Estado, mas também por Organizações Não-Governamentais (ONGs) e outras organizações sociais. Nesse sentido, este trabalho tem como objetivo principal analisar o papel das políticas públicas desenvolvidas em Uberlândia referentes ao planejamento urbano, avaliando a base histórico-estrutural para a realização dessa prática, bem como os aspectos sociais, econômicos e metodológicos, mais recentes que a permeiam.
Befundbuch 3.0. Befundansprache und Befundverortung durch semantische Technologien
Authors: Breitling Stefan , Arera-Rütenik Tobias , Giese Jürgen , Salzer Leonhard
Published: November 2022

Building archaeological findings are the primary sources of historic building research and an important basis for object-oriented interpretation in architectural history. In order to preserve the spatial, temporal and other content-related cross-references and contexts that are particularly valuable for analysis, a loose collection of diverse presentation formats such as descriptive texts, findings sheets, plan drawings and 3D representations has become established. However, this diversity is also a reason for the lack of accessibility and sustainable connectivity of this useful information. Linked Open Data technologies of the Semantic Web offer the possibility of making the findings available in a human- and machine-readable form, together with their diverse references. This requires consistent ›semantic modelling‹ of the material evidence in ontological form, oriented on specialist scientific needs.
Dynamic regulation of eEF1A1 acetylation affects colorectal carcinogenesis
Authors: Jiang Hongpeng , Zhang Yu , Liu Boya , Yang Xin , Wang Zhe , Han Meng , Li Huiying , Luo Jianyuan , Yao Hongwei
Published: November 2022

The dysregulation of the translation elongation factor families which are responsible for reprogramming of mRNA translation has been shown to contribute to tumor progression. Here, we report that the acetylation of eukaryotic Elongation Factor 1 Alpha 1 (eEF1A1/EF1A1) is required for genotoxic stress response and maintaining the malignancy of colorectal cancer (CRC) cells. The evolutionarily conserved site K439 is identified as the key acetylation site. Tissue expression analysis demonstrates that the acetylation level of eEF1A1 K439 is higher than paired normal tissues. Most importantly, hyperacetylation of eEF1A1 at K439 negatively correlates with CRC patient survival. Mechanistically, CBP and SIRT1 are the major acetyltransferase and deacetylase of eEF1A1. Hyperacetylation of eEF1A1 at K439 shows a significant tumor-promoting effect by increasing the capacity of proliferation, migration, and invasion of CRC cells. Our findings identify the altered post-translational modification at the translation machines as a critical factor in stress response and susceptibility to colorectal carcinogenesis.
Generación de residuos sólidos urbanos municipales y su relación con un indicador de ingreso municipal en México (años 2010 y 2015)
Authors: Ceballos Pérez Sergio Gabriel , Flores-Xolocotzi Ramiro
Published: November 2022

[Introduction]: The theoretical relationship between economic growth (measured through some income indicator) and the generation of pollutants explains that at a certain level of growth, a decrease in them begins, a process known as decoupling and that possibly indicates the presence of an Environmental Curve of Kuznets (CAK). [Objective]: A possible decoupling of municipal solid waste in Mexico was studied for the years 2010 and 2015 in 2 183 municipalities. [Methodology]: For this purpose, the independent variable used was the annual municipal income per capita in dollars expressed as purchasing power parities. In addition, the model was controlled by municipal population density and average municipal schooling. [Results]: The results of models estimated by random effects, fixed effects, and cluster robust standard errors in fixed effects show the probable existence of decoupling between income and municipal solid waste in the 2 183 municipalities studied. [Conclusions]: Although there is a possible CAK, new research is needed to contrast this hypothesis considering a long-time horizon at the national, regional, and sectorial levels in Mexico and Latin America too, as well as considering the effect of variables related to the collection of urban solid waste and recycling.
Uo mo aso uma, a o uso mo aso vale: Lessons from Aotearoa physiotherapists responding to disasters within the Pacific
Authors: Sanerivi Lilo Oka P. A. W. R. , Skinner Margot A.
Published: November 2022

Disasters can have sudden and devastating impacts on the health systems in the Pacific region, many parts of which are in a precarious state. The region is increasingly recognised as being prone to disasters such as those caused by climate change or epidemics. Physiotherapists have been identified as vital members of the interprofessional health team that responds to such catastrophic events. Despite this, in the Pacific region little is known about the nature of physiotherapists’ involvement and the multifaceted roles physiotherapists play in responding to disasters. This clinical commentary contains 1) an evaluation of the relevant literature sourced to describe the current knowledge base; and 2) a commentary on the experiences gained from physiotherapists’ response to the Samoa measles outbreak in 2019. Outcomes from the commentary have formed the basis of recommendations for the role the physiotherapy profession in Aotearoa New Zealand could have in responding to future potential disasters in the wider Pacific region.
Influence of the shape and dimensions of the chimney blocks for heat transfer in the regenerator of the glass furnace
Authors: Dzyuzer V. Ya.
Published: November 2022

Рассмотрено влияние формы и размеров дымоходных блоков на регенеративный теплообмен в стекловаренной печи с подковообразным пламенем. Исследованы дымоходные блоки с разным сочетанием размеров проходного сечения, высоты и толщины стенки. Для оценки эффективности блоков использованы параметры конструкторского расчета регенератора. Установлено, что наименьший объем и массу высокотемпературной зоны насадки, ограниченной температурой дыма 1100 оС, обеспечивают блоки TL 15/15 с удельным объемом 0,259 м3/м3 и удельной поверхностью нагрева 18,9 м2/м3. В зоне конденсации сульфатов и низкотемпературной зоне насадки следует использовать блоки TG 15/15, удельный объем и удельная поверхность нагрева равны 0,296 м3/м3 и 17,4 м2/м3 соответственно.
Development of super-covered slagresistant aluminosilicate refractory
Authors: Sidorina E. A. , Isagulov A. Z. , Kashcheev I. D. , Zemlyanoi K. G.
Published: November 2022

На основе алюмосиликатного сырья Республики Казахстан предложена технология получения сверхплотных алюмосиликатных огнеупоров с повышенной шлакоустойчивостью для тепловых процессов утилизации фторсодержащих углеродистых отходов. Использование «керамической» технологии позволяет получать структуру с водопоглощением менее 1 % как у шамота, так и у огнеупорных изделий с разным содержанием Al2O3.
THE EFFECTS OF SOWING DESIGNS ON FORAGE YIELD AND QUALITY OF SWEET SORGHUM AND MUNG BEAN MIXTURES UNDER MEDITERRANEAN CONDITIONS
Authors: ERTEKİN İbrahim , YILMAZ Şaban
Published: November 2022

This study was conducted to determine the effects of sowing designs on forage yield and quality in sweet sorghum and mung bean grown as binary mixture with different cultivation systems in Mediterranean conditions under second crop season during 2019-2020. As sowing design, twin-row (20×55 cm row spacing), narrow-row (55 cm row spacing) and conventional-row (75 cm row spacing) were used as sowing design. The mixtures were formed based on the plant density and alternative row numbers of sweet sorghum and mung bean. Sowing was done on alternating rows of 1 row of sweet sorghum and 1 row of mung bean and 2 rows of sweet sorghum and 1 row of mung bean. The plant density of sweet sorghum was 14 plants m-2 and plant densities of mung bean were 14 plants m-2, 21 plants m-2 and 28 plants m-2. This experiment was planned as two-factor (sowing designs and mixtures) and conducted in randomized complete block design arranged in split plot with 3 replications. To evaluate the forage yield and quality, fresh forage yield, dry matter yield, NDF, ADF, ADL, crude ash crude protein and ether extract characteristics were examined. In addition, the intercropping potential of mung bean and sweet sorghum mixtures was evaluated by the land equivalent ratio. As a result of the present study, mixed cultivation of 14 plants m-2 with one row of sweet sorghum and 14 plants m-2 with one row of mung bean gave the best results in narrow row sowing design. It was concluded that an efficient and high quality intercropping system can be realized in the second crop conditions in regions where Mediterranean climatic conditions prevail, especially for mung beans and sweet sorghum.
GRAIN YIELD AND NUTRITIONAL QUALITY OF DIFFERENT RYE GENOTYPES
Authors: BAŞARAN Uğur , GÜLÜMSER Erdem , KARDEŞ Yusuf Murat , ÇOPUR DOĞRUSÖZ Medine , MUT Hanife
Published: November 2022

Rye is resistant to cold, drought, diseases, and pests, and it protects and improves the soil structure. The aim of this study was to determine the grain yield and quality of rye (Secale cereale) as feed in Central Anatolian conditions. For this purpose, nine populations and one variety (Aslım-95) of rye and one variety of triticale (Karma-2000) were investigated for grain yield, crude protein content, acid detergent fiber (ADF), neutral detergent fiber (NDF), potassium, phosphorus, calcium, magnesium, total phenolic, total flavonoid, DPPH free radical scavenging activity and condensed tannin content. Experiments were carried out in Yozgat ecological conditions in 2018-2019 and 2019-2020 growing seasons in a randomized block design with three replications. There were significant (P
Preface
Authors: Supera-Markowska Maria
Published: November 2022

The purpose of this paper which serves as an introduction to the special edition of the quarterly of “Finanse i Prawo finansowe. Journal of Finance and Financial Law” under the title of Current Challenges for National Tax Policies and International Tax Coordination – Selected Issues from the Polish, Spanish and International Perspectives, is to outline major issues and challenges in shaping national and international tax policies. The research hypothesis is that, to a large extent, the economic consequences of the COVID-19 pandemic, but also the changing economic reality associated with the progressive internationalization and digitalization of business activity, pose numerous and significant challenges in the field of shaping tax policies, both at the national and international level. The research was carried out using a dogmatic-legal and legal-comparative method, taking into account the provisions of law, the body of literature as well as some economic data. The result of the research is to establish a starting point for further analysis of selected topics that reflect the major current challenges for national tax policies and international tax coordination. They include the question of legitimacy of taxes, the issues of correct realisation of the fiscal function of taxes while at the same time implementing the basic tax principles, the matter of tax compliance and the question of tax policy-shaping within the framework of the democratic law-governed state. Those issues presented from the Polish and Spanish perspectives, hopefully may provide an interesting contribution and make a valuable input to further discussions on the issue of shaping tax policies, both at the national and international levels.
Elementos de la teoría del tráfico vehicular
Authors: Fernández A. Rodrigo , Dextre Quijandría Juan Carlos
Published: November 2022

El tránsito o tráfico es la circulación de personas, algunas de ellas en vehículos, por el espacio público. Se trata de un fenómeno físico y, a la vez, social. Estamos convencidos de que cualquier análisis de los problemas del tránsito urbano parte del reconocimiento de las bases conceptuales de este fenómeno. A estas bases conceptuales las llamaremos teoría del tráfico vehicular. Una vez entendidos los elementos de la teoría del tráfico vehicular los podemos aplicar a lo que se denomina la ingeniería de tránsito, que es la aplicación de técnicas de la ingeniería para aminorar los impactos sociales, urbanos y ambientales derivados del tráfico. Los diseños conceptuales propuestos por la ingeniería de tránsito deben ser traducidos a planos de ingeniería que realizan los diseñadores viales. Son estos planos los que, finalmente, se materializan en las calles. En consecuencia, ningún dispositivo vial o medida de gestión de tráfico será acabado si no se conocen los principios aportados por la teoría que aquí se esboza. Este texto presenta los principios para el estudio de la circulación de vehículos. Está concebido para estudiantes de habla hispana de cursos avanzados de ingeniería civil o de posgrado en ingeniería de transporte.
Impact of Inclusive Leadership on Innovative Work Behavior: The Mediating Role of Job Crafting
Authors: Guo Yinping , Jin Junge , Lim Sanghyuk
Published: November 2022

The study aims to examine the mediating role of job crafting between inclusive leadership and innovative work behavior. The data were collected from 314 workers employed in China's small and medium-sized industries. The data collection was done through survey design. The data analysis was done through structural equation modeling using Spss 26 and Mplus 8. Inclusive leadership was found to be related to job crafting and innovative work behavior of the employees. Job crafting was found to be mediating between inclusive leadership and innovative work behavior. The study delineated the link mechanism between inclusive leadership and innovative work behavior. Studying inclusive leadership in the context of Chinese culture is a powerful complement to inclusive leadership theory. This paper provides the managers of SMEs with significant managerial insights into how inclusive leadership can effectively motivate employees’ innovative work behaviors.
Asociación entre los factores de riesgo al Virus Papiloma Humano (VPH) sobre los conocimientos y casualidad del VPH al cáncer de cuello uterino en mujeres en edad fértil del Perú 2019
Authors:   , Cardoso Contreras Shirley Katherine , Caycho Porta Diana Carolina
Published: November 2022

Objetivos: Analizar la asociación entre los factores de riesgo al Virus Papiloma Humano y el conocimiento sobre Cáncer de cuello uterino y conocimiento y causalidad del Virus Papiloma Humano sobre el cáncer en mujeres en edad fértil del Perú durante el año 2019. Métodos: Se realizó un estudio retrospectivo, transversal y observacional de tipo analítico, de análisis secundario de base de datos proporcionada por la Encuesta Demográfica y Salud Familiar 2019. Resultados: Este estudio analizó datos de 19312 femeninas que se encontraban en el rango de edad de 15 a 49 años. Se encontró que un 8,7% había empezado su actividad sexual antes de los 14 años y que además, no creía que el Virus Papiloma Humano cause Cáncer de cuello uterino (IC95% 5,0-14,4), un 46,6% no había utilizado condón con su última pareja sexual pese a escuchar acerca del Cáncer de cuello uterino (IC95% 42,8-50,3), el 45,3% uso condón con su última pareja sexual y creían que el Virus Papiloma Humano causaba Cáncer de cuello uterino (IC95% 41,0-49,8), el 54,7% que había tenido más de 2 parejas sexuales en 12 meses había oído hablar sobre el Cáncer de cuello uterino (IC 95% 53,3-56,1), y el 57,1% creía que el Virus Papiloma Humano causaba Cáncer de cuello uterino (IC 95% 55,3-58,9). Conclusiones: Nuestro estudio mostró que aún persiste el desconocimiento acerca de que el Virus Papiloma Humano cause Cáncer de cuello uterino en la población a pesar de las constantes campañas de promoción por parte del Ministerio de salud.
A confidence ellipse analysis for stochastic dynamics model of Alzheimer’s disease
Authors: Gao Jianzhong , Ji Ju-Ping , Liu Yan-Ping , Zhang Feng , Wang Rui-Wu , Wang Hao
Published: November 2022

The Alzheimer’s disease (AD) is a neurodegenerative disease, which is caused by the aggregation of beta-amyloid peptide (Aβ) in the patient’s brain and the disorder of Ca2+ homeostasis in neurons. Caluwé and Dupont [1] proposed a deterministic AD model to explore the effect of Ca2+ on AD. They demonstrated the positive feedback loop between Aβ and Ca2+ and the occurrence of bistability. Based on their results, we further discuss the bistable behaviors. We present two periodically feasible drug strategies to alleviate the AD and screen out more effective one. In this paper, we also formulate a stochastic AD model, analyze the existence and uniqueness of global positive solutions and establish sufficient conditions for the existence of ergodic stationary distribution. Furthermore, the confidence ellipses describing the configurational arrangement of stochastic coexistence equilibria are constructed by stochastic sensitivity function technique, and tipping threshold is estimated as well. Noise-induced stochastic switching between two coexistence equilibria is observed in bistability region. Our results provide a new idea to control noise to alleviate AD through physical therapy and drug therapy.
Dynamic Simulation of Carbon Emission under Different Policy Scenarios in Pearl River Delta Urban Agglomeration, China
Authors: Ding Yi , Yin Jian , Jiang Hongtao , Xia Ruici , Zhang Bin , Luo Xinyuan , Wei Danqi
Published: November 2022

Pearl River Delta Urban Agglomeration (PRDUA) is a key region in China's economic development and ecological civilization construction, and also an important global economic cluster area. The carbon emission (CE) prediction can provide a basis for local governments to formulate sustainable development policies. In order to make more accurate predictions of CE in the PRDUA, the STIRPAT model was employed to classify CE influencing factors into seven dimensions, on the basis of which a system dynamics model was constructed to analyze the multi-dimensional impact of CE. Then, 21 CE prediction scenarios were created at the city level based on the spatial heterogeneity analysis of the CE factors by Multiscale Geographically Weighted Regression (MGWR). The results showed that the PRDUA as a whole will reach carbon peak (CP) by 2025. Shenzhen, Zhuhai and Dongguan will reach CP before 2020, while other cities will reach CP by 2025–2030. The government policy constraints can effectively curb CE, but if government constraints were relaxed, CE will rise and individual cities will not reach CP. Comprehensive CE reduction policies are better than a single CE reduction policy. Finally, the study discussed the relationship between economic development and CE, and put forward some policy suggestions for CE reduction.
A Study on Multi-agent Reinforcement Learning for Autonomous Distribution Vehicles
Authors: Ergün Serap
Published: November 2022

A self-driving car, also called an autonomous vehicle, is capable of sensing the environment and driving without any human intervention, utilizing software algorithms and a fully automated driving system. This allows the vehicle to manage external conditions similar to a human. Safe and efficient delivery services require road controls and road restrictions to prevent accidents and reduce damage from unexpected technical failures. This study formulates the Autonomous Delivery Vehicles optimization problem and proposes a multi-agent reinforcement learning method using the analytically calculated shortest path information.
Quantifying the Impact of the COVID-19 Pandemic on Clinical Trial Enrollment Rates Over Time in 37 Countries
Authors: McDonald Kelsey , Saini Kamal S. , Lu Mary , Gaisenband Stefan Diaz , Fletcher Cassandra , McLeroth Patrick , Seltzer Earl
Published: November 2022

The COVID-19 pandemic has had an unprecedented and disruptive impact on people’s health and lives worldwide. In addition to burdening people’s health in the short-term in the form of infection, illness, and mortality, there has been an enormous negative impact on clinical research. Clinical trials experienced challenges in ensuring patient safety and enrolling new patients throughout the pandemic. Here, we investigate and quantify the negative impact that the COVID-19 pandemic has industry-sponsored clinical trials, both in the United States and worldwide. We find a negative correlation between the severity of the COVID-19 pandemic and clinical trial enrollment rate, with the relationship being strongest during the first three months of the pandemic compared to the entire duration of the pandemic. This negative statistical relationship holds across therapeutic areas, across states in the US despite the heterogeneity of responses at the state-level, and across countries. This work has significant implications for the management of clinical trials worldwide in response to the fluctuating severity of COVID-19 moving forward, and for future pandemics.