bluuebunny/crossref_metadata_2025 huggingface dataset:
LncRNA NEAT1 exacerbates sepsis-induced acute lung injury via targeting miR-26a-5p/ROCK1 axis
Authors: Fan Xiu-Ying , Tang Li-Bin , Ma Zhong-Xu , Shen Han-Zhang , Qi Fei , Xia Jia-Wei
Published: August 2022

Background: Acute lung injury (ALI) is a severe disease of respiratory system, which one of the most common causes is sepsis induction. Cell apoptosis and inflammation contribute to the progression of ALI and lncRNAs play crucial roles in ALI. Hence, this study is to unearth the specific mechanism of NEAT1.Methods: BEAS-2B cells was exposed to lipopolysaccharide (LPS) to construct cell model of sepsis-induced ALI. The gene and protein expression were evaluated using qRT-PCR and western blot. Cell viability was determined by CCK-8. Cell apoptosis was detected utilizing flow cytometry. The secretion of inflammatory factors was assessed using ELISA. The interconnections among NEAT1, miR-26a-5p and ROCK1 were validated using starbase, luciferase assay and RIP.Results: LPS treatment elevated NEAT1 and ROCK1 levels while reduced miR-26a-5p level in BEAS-2B cells. Besides, LPS treatment augmented cell apoptosis and enhanced inflammatory cytokine secretion, whereas NEAT1 silencing could reversed LPS-mediated these influences in BEAS-2B cells. Mechanistically, NEAT1 positively mediated ROCK1 expression through targeting miR-26a-5p. Moreover, miR-26a-5p inhibitor offset NEAT1 depletion-mediated suppressive influences on cell apoptosis and inflammation. ROCK1 upregulation attenuated the inhibitory impacts generated by miR-26a-5p overexpression on cell apoptosis and inflammation. Conclusion: Our results revealed NEAT1 could reinforce LPS-induced cell apoptosis and inflammatory response through repressing miR-26a-5p/ROCK1 axis, thereby aggravating ALI caused by sepsis. Our data suggested NEAT1, miR-26a-5p and ROCK1 might be biomarkers and targets genes for alleviating sepsis-induced ALI.
Evaluation of Intraoperative Navigation-assisted Thoracoscopic Oesophagectomy Outcomes in the Patient's Semi-prone Position
Authors: Khorobykh Тatyana , Dydykin Sergey , Mishchenko Natalya , Kapitonova Мarina , Gupalo Sergey , Kytko Olesya , Bogoyavlenskaya Тatyana , Agadzhanov Vadim , Salikhov Рashad
Published: August 2022

Video-assisted thoracoscopic esophagectomy for oesophageal cancer involving the thoracic region has certain advantages. However, thoracoscopic instrument manipulations performed in the narrow confines of the posterior mediastinum carry the risk of intraoperative injury to other vital structures.Purpose of the studyTo improve surgical treatment outcomes of thoracic oesophageal cancer patients using topographic anatomical navigation.Materials and methodsThe study included 23 patients with stages II-III middle and lower thoracic oesophageal cancer (TOC), according to TNM-8. All of them underwent thoracoscopic oesophagectomy in the semi-prone position. While the 1st group of patients (n=15) underwent conventional surgery, in the 2nd group of patients (n= 8), our algorithm of sequential visual navigation based on topographic and anatomical landmarks was employed.ResultsIn the 1st group,4 patients (26.7%) developed intraoperative complications. Out of these four, in 3 patients, injury of the thoracic lymphatic duct complicated with chylothorax development was diagnosed, requiring reoperations; in one patient, the surgery caused damage to the thoracic aorta. Unfortunately, two patients out of these four died.In contrast, there were no intraoperative complications or deaths in the 2nd group. In addition, the algorithm allowed the removal of 18% more lymph nodes, reduction in the incidence of postoperative pneumonia by 25%, the operation time by 25±15 minutes, and the length of stays by 3±2 days, compared with the 1st group of patients. ConclusionTopographic anatomical navigation can reduce the incidence of intra- and postoperative complications, effectively guide lymph node dissection, and shorten hospital stay.
Comparison of Quality of Life in patients with advanced chronic kidney disease undergoing haemodialysis, peritoneal dialysis and conservative management in Johannesburg, South Africa: a cross-sectional, descriptive study
Authors: Mathew Neelu , Davies Malcolm , Kaldine Feroza , Cassimjee Zaheera
Published: August 2022

Introduction:
Mental health and quality of life are under-appreciated clinical targets which affect patient and modality survival. Lack of dialysis slot availability in the resource-constrained public health sector in South Africa, results in assignment to treatment modalities without regard to effects on these parameters. We assessed the effect of dialysis modality, demographic and laboratory parameters on mental health and quality of life measurements.
Methods
Size-matched voluntary cohorts were recruited from patients on haemodialysis (HD), peritoneal dialysis (PD), and patients on conservative management (with an estimated glomerular filtration rate below 20mL/min/1.73m2), between September 2020 and March 2021. Responses to self-administered Hospital Anxiety and Depression Scale (HADS) and Kidney Disease Quality of Life Short Form 36 (KDQOL-SF36) questionnaires and demographic and baseline laboratory parameters were compared between treatment modalities using the Student t-test and Pearson Chi-square test. Linear regression was used to test for independent effect where significant difference was observed.
Results
HADS anxiety score was highest (p < 0.001) and KDQOL-SF36 emotional wellbeing was poorer in HD (p < 0.001). Social functioning (p = 0.011) and physical limitation due to pain (p = 0.030) were poorer in PD. Unemployment (p = 0.044) was more frequent in HD; fewer PD patients required a social support grant (p = 0.008). Significant independent effect was found for age (p = 0.009), employment (p = 0.007), and haemoglobin (Hb) (p = 0.025) on anxiety; HD worsened (p = 0.037) and PD improved (p = 0.007) anxiety. Unemployment (p < 0.001) and low Hb (p = 0.018) worsened depression. PD improved (p = 0.002) and HD worsened (p < 0.001) emotional well-being. PD worsened social functioning (p = 0.002). PD (p = 0.007) and higher phosphate (p = 0.022) worsened and HD (p = 0.01) and higher Hb (p = 0.02) improved physical discomfort / pain.
Conclusion
Advanced chronic kidney disease increases anxiety and depression and limits quality of life. Peritoneal dialysis improves mental health and emotional wellbeing and preserves the ability to undertake economic activity but limits social functioning and causes greater physical discomfort. Targeting haemoglobin and phosphate may ameliorate modality effects on mental health and quality of life.
The postoperative prognosis of elderly intertrochanteric fracture patients as evaluated by the Chang reduction quality criteria
Authors: He Miao , Liu Jian , Deng Xu , Zhang xiaoxing
Published: August 2022

Objective
The aim of this study was to investigate the relationship between the Chang reduction quality criteria (CRQC) and the outcome of elderly intertrochanteric fractures based on a subgroup of follow-up time.
Methods
Retrospective analysis of 389 elderly patients with intertrochanteric fractures treated surgically from January 2019 to June 2021, including 130 males and 259 females; age 84.64 (77.50-89.74) years. Patient survival was followed by telephone between admission to hospital for fracture and death, or until study deadline (June 1, 2022). According to the CRQC, were divided into Poor, Acceptable, Excellent three groups. Based on total follow-up time and 1-year follow-up, univariate and multivariate Cox proportional hazard models were used to assess the association between CRQC and all-cause mortality in elderly intertrochanteric fractures. Further subgroup analysis was performed according to different clinical and biological characteristics to make the results more accurate.
Results
The mortality were 24.68% and 15.42% at 1 year and total follow-up, respectively. In the total follow-up time and 1-year follow-up time, the mortality of CRQC-Excellent was significantly lower than that of CRQC-Acceptable (p.adj < 0.05), and was significantly lower than that of CRQC-Poor (p.adj < 0.05). After multi-factor adjustment, CRQC-Acceptable and Poor in were independent risk factors affecting the overall mortality and 1-year mortality. In addition, advanced age, comorbidities ≥ 1, ASA3 + 4, and prolonged preoperative waiting time were independent risk factors for survival in the total follow-up time. At 1-year follow-up, only ASA3 + 4 and prolonged preoperative waiting time were independent risk factors for survival. Subgroup analysis according to different characteristics at the total follow-up time and at the one-year follow-up time showed that in most subgroups as CRQC decreased was significantly associated with an increase in all-cause mortality (p for trend < 0.05).
Conclusions
This study highlights that CRQC-Acceptable and Poor are associated with increased all-cause mortality in elderly intertrochanteric fractures. We should try to achieve good reduction of these fractures.
The efficacy and safety of topical saline-irrigation with tranexamic acid on perioperative blood loss in patients treated with percutaneous endoscopic interlaminar discectomy
Authors: yu zhang , Zhao Wenjie , Hu Man , Liu Xin , Peng Qing , Meng Bo , Yang Sheng , Feng Xinmin , Zhang Liang
Published: August 2022

Background: Adequate visualization in percutaneous endoscopic interlaminar discectomy (PEID) surgery is essential for the successful completion of lumbar discectomy. However, there is currently no relevant research on the efficacy and safety of adding tranexamic acid (TXA) to the saline irrigation fluid in PEID surgery. To evaluate the efficacy and safety of topical saline-irrigation with TXA for PEID in the treatment of lumbar disc herniation.Methods: Patients who underwent PEID surgery for L5-S1 lumbar disc herniation were divided into two groups. PEID was performed with saline-irrigation fluid containing 0.33 g of TXA per 1 L of saline in the TXA group (n=38) and only saline in the control group (n=51). All PEID surgeries were performed by the same spine surgery team. The hidden blood loss (HBL), intraoperative blood loss (IBL), total blood loss (TBL), amount of fluid used, operation time, visual clarity, hospital stay, blood transfusion rate, coagulation index and complication rate were compared between the two groups.Results: The TBL, HBL, and IBL in the TXA group were significantly lower than those of the control group. The postoperative hemoglobin in the TXA group was significantly higher than that of the control group. Visual clarity was significantly better and operation time was significantly shorter in the TXA group. However, there was no significant difference in postoperative hematocrit, blood coagulation function, amount of fluid used, blood transfusion rate and perioperative complications between the two groups.Conclusion: In PEID surgery, addition TXA to topical saline-irrigating fluid can significantly reduce HBL, IBL and TBL. Addition TXA to topical saline-irrigating fluid can improve visual clarity in the surgery and reduce operation time, but do not change coagulation function or increase complication rate.
CD206+ mono-macrophages are involved in Antineutrophil Cytoplasmic Antibodies associated glomerulonephritis
Authors: AN Xiao-ning , Wei Zhao-Nan , Xie Yin-Yin , Xu Jing , Shen Yan , Ni Li-Yan , Shi Hao , Shen Ping-Yan , Zhang Wen , Chen Yong-Xi
Published: August 2022

BackgroundAntineutrophil Cytoplasmic Antibodies (ANCA) associated glomerulonephritis (AGN) is a group of autoimmune diseases and mono-macrophages are involved in its glomerular injuries. We investigate role of CD206+ mono-macrophages in AGN to provide therapeutic targets for the disease. Methods 27 AGN patients (14 active AGN, 13 remissive AGN) together with healthy controls (n=9), disease controls (n=6) and kidney function adjusted controls (n=9) from Department of Nephrology, Ruijin hospital were recruited. Flow cytometry was used to study proportion of CD206+ cells in peripheral blood. Immunohistochemistry for CD206 staining was performed in kidney tissues. Serum soluble CD206 (sCD206) was measured by enzyme-linked immunosorbent assay (ELISA). We also generated murine myeloperoxidase (MPO) (muMPO) ANCA by immunizing Mpo-/- mice. Mouse bone marrow-derived macrophages (BMDMs) and peripheral blood mononuclear cell (PBMC) derived macrophages were treated with MPO ANCA with or without its inhibitor AZD5904 to investigate the effects of MPO-ANCA on CD206 expression.ResultsThe proportion of peripheral CD206+CD68+ cells in active AGN patients were significantly higher than that in remissive patients (p<0.001), healthy controls (p<0.001) and kidney function adjusted controls (p<0.001). Serum sCD206 level in active AGN patients was higher than that in healthy controls (p<0.05) and remissive patients (p<0.01). Immunohistochemistry showed CD206 was highly expressed in the kidney tissues in active AGN patients in comparison with disease controls. Further studies showed MPO ANCA could induce CD206 expression in BMDMs and PBMC derived macrophages and such effects could be reversed by its inhibitor AZD5904. Conclusion ANCA could induce CD206 expression on mono-macrophages and CD206+ mono-macrophages are activated in AGN. Serum sCD206 correlates to disease activity and it could serve as a potential therapeutic target for the disease.
EGFR-dependent aerotaxis is a common trait of breast tumour cells
Authors: MIKAELIAN Ivan , GADET Rudy , DEYGAS Mathieu , Bertolino Philippe , Hennino Anca , Gillet Germain , Rimokh Ruth , Berremila Sid-Ali , PEOC'H Michel , GONZALO Philippe
Published: August 2022

Background: Aerotaxis, the chemotactism to oxygen, is well documented in prokaryotes. We previously reported for the first time that non-tumorigenic breast epithelial cells also display unequivocal directional migration towards oxygen. This process is independent of the hypoxia-inducible factor (HIF)/prolyl hydroxylase domain (PHD) pathway but controlled by the redox regulation of epidermal growth factor receptor (EGFR), with a reactive oxygen species (ROS) gradient overlapping the oxygen gradient at low oxygen concentration. Since hypoxia is an acknowledged hallmark of cancers, we addressed the putative contribution of aerotaxis to cancer metastasis by studying the directed migration of cancer cells from their hypoxic environment towards nearby oxygen sources, modelling the in vivo migration of cancer cells towards blood capillaries.Methods: We subjected to the aerotactic test described in our previous papers cells isolated from fresh breast tumours analysed by the Pathology Department of the Saint-Etienne University Hospital (France) over a year. The main selection criterion, aside from patient consent, was the size of the tumour, which had to be large enough to perform the aerotactic tests without compromising routine diagnostic tests. Finally, we compared the aerotactic properties of these primary cells with those of commonly available breast cancer cell lines.Results: We show that cells freshly isolated from sixteen human breast tumour biopsies, representative of various histological characteristics and grades, are endowed with strong aerotactic properties similar to normal mammary epithelial cell lines. Strikingly, aerotaxis of these primary cancerous cells is also strongly dependent on both EGFR activation and ROS. In addition, we demonstrate that aerotaxis can trigger directional invasion of tumour cells within the extracellular matrix contrary to normal mammary epithelial cells. This contrasts with results obtained with breast cancer cell lines, in which aerotactic properties were either retained or impaired, and in some cases, even lost during the establishment of these cell lines.Conclusions:Altogether, our results support that aerotaxis may play an important role in breast tumour metastasis. In view of these findings, we discuss the prospects for combating metastatic spread. Trial registration: IRBN1462021/CHUSTE
Use of the Superblock Model for Physical Activity in Barcelona: A one-year observational comparative study using SOPARC
Authors: Puig-Ribera Anna , Arumí-Prat Ignasi , Cirera Eva , Solà Marta , Codina Anna , Palència Laia , Biaani Brenda , Pérez Katherine
Published: August 2022

Background: The Barcelona Superblock model transforms urban public spaces into active-friendly spaces, a key issue for public health. This study assessed the use of a newly developed Superblock in St. Antoni Market Square for physical activity and sedentary behaviour during the first year of implementation. It then compared citizens’ use of the Superblock for physical activity and sedentary behaviour with a comparison site at one-year follow-up, when the Superblock was fully integrated into citizens’ daily life. Methods: This observational comparative study (May 2018-May 2019) used the System for Observing Play and Recreation in Communities (SOPARC). SOPARC assessed citizens’ sitting, standing, walking, vigorous activities and use of electric scooter by gender, age group and time of the day. At the Superblock site, two observers completed five weekly observations: the opening week, and at three, five, eight and twelve months. At the comparison site, observers completed one weekly observation at twelve months after the implementation of the Superblock. Observations included 4 days/week (including weekends) and, 4 hours/day (morning, midday, afternoon, evening). Results: At baseline, an average of 2,340 citizens/hour were observed using the Superblock but visits reduced by 12% in the next three observation weeks and 17.6% after one-year (mainly elderly and teenagers). At baseline, 92.9% walked in the Superblock, while 3.1% engaged in vigorous physical activity. After one year, citizens’ walking decreased by 18.2%, from 2,170 citizens/hour at baseline to 1,930 citizens/hour. Citizens’ engagement in vigorous activities also declined by 11%, from 73 citizens/hour at baseline to 65 citizens/hour at one-year follow up. In the comparison site, citizens’ usage for walking and vigorous physical activity was similar to the Superblock. Conclusions: This is the first study to assess citizens’ usage for physical activity of the Barcelona Superblock model, an urban built-environment intervention that is both novel and health-enhancing. The Superblock model would benefit from strategies maximizing effectiveness for promoting superblock-based physical activity, with special focus on seniors and teenagers.
Assessment of the Quality of Economic Growth in Agriculture
Authors: Abduvakhidov Akmal , Eshpulatov Dostonbek , Olimovna Kadyrova Dilafruz
Published: August 2022

The issue of economic growth is one of the main macroeconomic problems that have always been of interest to economists. Also, in the current macroeconomic situation, another side of the problem is being manifested. That is, the current issue is not only economic growth, but also the study of the factors that determine its quality has become an urgent issue. The existing theories of economic growth cannot fully explain the matter. Therefore, the article studied the issues of qualitative economic growth and evaluated statistical indicators that determine it. Indicators such as material return, capital return and labor productivity were selected as the main indicators that ensure the quality of economic growth in agriculture. Conducting the research, we found out that the indicator of material return and capital return in agriculture has been decreasing. Moreover, the exact conclusions and suggestions were made on ensuring quality economic growth in agriculture.
Comparison of Clinical and Radiological Outcome of Radial Shortening and Capitate Shortening in the Early Stages of Kienböck Disease
Authors: Nazari Dariush , Kachooei Amir R. , Rezaeian Amin , Birjandinejad Ali , Ariamanesh Amir Shahriar , Moradi Ali
Published: August 2022

Background: This study aims to compare the clinical and radiological outcomes of radial shortening osteotomy (RSO) and capitate shortening osteotomy (CSO) in the early stages of Kienböck disease. Methods: Grip strength and wrist range of motion were assessed bilaterally. Also, the disease stage in pre-op and the final follow-up were determined. Quick-DASH, Patient-Rated Wrist Evaluation (PRWE), and Modified Mayo Wrist Score were used to assess patient comfort and function.Results: 23 patients were followed up (13 patients with RSO and 10 patients with CSO) for mean 46 months. Affected wrist range of motion in flexion and extension and grip strength was significantly lower than the unaffected side. Pain score in the Mayo wrist questionnaire was significantly lower in the RSO group than CSO group. The failure rate was 1.16% and 2.59% per year for RSO and CSO, respectively. Radiologic stage worsened in two patients (one in each RSO and CSO groups) and it was improved in nine patients (six in RSO and three in CSO groups). Six patients (three in each RSO and CSO groups) underwent revision surgeries due to residual pain. As expected, wrist motion arc and grip strength were significantly more limited in these patients in comparison to others (p=0.031 and p=0.026, respectively).Conclusion: We found no significant differences between the two groups in terms of clinical findings, patients’ function and satisfaction, and success rate. Level of evidence: III - Retrospective cohort study
Radiation green synthesis of Guar gum -Pectin/ Polyacrylamide/ zinc oxide superabsorbent hydrogel composites for sustainable agriculture
Authors: Sayed Asmaa , Mohamed Mai M. , Abdel-Raouf Manar , Adel Ghada
Published: August 2022

In the current study, the performance of superabsorbent hydrogel composites comprised of Guar gum-Pectin/ Polyacrylamide/ ZnO crosslinked with gamma irradiation (10 kGy) has been investigated for sustainable agriculture. The claimed composites (GG/PC/PAAm/ZnOx) were characterized by FTIR, TGA, and AFM. The swelling capacity data reveal that the equilibrium water swelling (EW) of the composites was increased by increasing the ZnO content from 600 g/g to 1050 g/g for zero to the highest concentration of ZnO, respectively. Furthermore, the physical properties of the soil mixed with the hydrogels were improved; water holding capacity (WHC) increased to 66% and water retention (WR) kept at 15% after 20 days. The composites showed a good degradability in the biodegradation test. They also portrayed super-absorption capacity at three swelling/deswelling cycles. This advancement is important for reducing water consumption through the irrigation of arid lands. The prepared composites were proved as excellent candidates in sustainable agriculture applications.
Highly accurate species identification of eastern Pacific rockfishes (Sebastes spp.) with high-throughput DNA sequencing
Authors: Baetscher Diana S. , Nuetzel Hayley , Garza John Carlos
Published: August 2022

Genetic species identification is often necessary for species flocks, such as rockfishes in the genus Sebastes (Teleostei, Scorpaenidae), where traditional visual identification methods are challenged by the presence of many sympatric species with morphologically similar juveniles. Here we present a straightforward approach for performing species identification in rockfishes using 96 nuclear microhaplotype loci that can be efficiently genotyped using high-throughput DNA sequencing. Self-assignment of nearly 1 000 samples from 54 species resulted in > 99% accurate species identification at a 95% confidence threshold. Phylogenetic relationships of Sebastes uncovered with these same loci were highly concordant with relationships previously derived primarily with mitochondrial DNA. We also assessed ascertainment bias and consequent reduced nucleotide diversity and heterozygosity in non-ascertainment species to understand the potential utility of these markers for those species. The data and protocol presented here will be useful for research and management of rockfishes in the northeastern Pacific Ocean.
Metastatic Colorectal Cancer as the Primary Phenotype in a Hereditary Breast and Ovarian Cancer Patient with Germline BRCA1 Mutation: A Case Report
Authors: Liu Ying , Zhu Jing , Wei Xiao , Yang Duoxia , Li Si , Qian Xiaoping , Li Li
Published: August 2022

Hereditary breast and ovarian cancer (HBOC) syndrome has increased predisposition to breast and/or ovarian cancer, and 24% of families with HBOC were associated with the germline pathogenic variants in BRCA1/2. Timely diagnosis and identification of mutation carriers is of utmost importance to improve survival benefit and quality of life. Cancers that has been included into screening of BRCA1/2 associated HBOC included prostate and pancreatic cancers etc.. In this case, we reported a patient who first presented symptom of CRC and was finally diagnosed as BRCA1 associated HBOC with advanced peritoneal carcinoma. With strategies of cetuximab based treatment and olaparib, and debulking surgeries, she has achieved an overall survival (OS) > 35 months. The aim was to indicate that HBOC might also first present as CRC, and comprehensive next-generation sequencing analysis might be a key complement for screening and diagnose of HBOC.
Evaluation of serum Fe concentration as an alternative biomarker for inflammation in cattle
Authors: MURAKAMI Yoshiki , TSUKANO Kenji , HIRATA Haruyuki , SUZUKI Kazuyuki
Published: August 2022

This study aimed to clarify the relationship between acute phase protein (APP) concentrations and serum Fe concentrations to determine whether serum iron (Fe) can be clinically applied as a substitute for APPs in cattle. One hundred and five Holstein-Friesian breed dairy cows were enrolled in this study. The cows were divided into forty-three healthy cows and sixty-two cows with inflammatory diseases. The plasma haptoglobin (HPT), alpha-1 acid glycoprotein (AGP), serum amyloid A (SAA), serum Fe levels, and other biochemical parameters in the cows were measured. From the results, HPT and AGP were significantly higher in inflammatory disease cattle compared with the control group, but no odds ratios were determined in the binomial analysis. Significant differences in SAA and serum Fe concentrations were observed between the inflammatory disease and control groups and odds ratios were determined in the binomial analysis. In addition, the cutoff value of Fe could be used to diagnose the inflammatory disease to the same degree as the cutoff value of SAA. The results suggested that serum Fe can be used as a substitute for APPs in the production of animal medicine.
Insights into a novel pathway of paraben-induced endotoxin affecting human sperm quality: Parabens’ dual effects of antibacterial and estrogenic activity
Authors: Zhang Linyuan , Song Pengkun , Guo Ying , Ding Chunguang , Xu Jianfeng , Yuan Dong , Qiao Peihuan , Huo Junsheng , Huang Jian , Chang Bing , Liang Xiaowei , Ding Gangqiang
Published: August 2022

Background
The decline of human sperm quality has caused great concern. Whether exposure to parabens and the intestinal endotoxin (IE) they induce are related to sperm quality is a challenging scientific question.
Results
We measured seminal methyl-(MeP), ethyl-(EtP), propyl-(PrP), and butyl-(BuP) parabens, their metabolites PHBA and 3,4-DHB, semen parameters, and blood IE (lipopolysaccharide, LPS) of 315 healthy men. Bivariate correlation and multiple linear regression were used to evaluate their interrelationships. For the first time, we found that MeP, EtP, PrP, BuP, PHBA, and 3,4-DHB in semen were negatively correlated with sperm parameters (r = − 0.184 to − 0.438), with EtP showing the strongest correlation. LPS was detected in all plasma samples (0.021–0.195 EU/mL) and negatively correlated with sperm concentration and total sperm count with R2 = 0.295 and R2 = 0.236, respectively, when LPS was higher than 0.104 EU/mL. LPS was positively correlated with EtP and BuP.
Conclusion
We concluded that seminal parabens affected sperm quality possibly due to estrogenic activity. Parabens likely promote intestinal endotoxemia and affect sperm quality in healthy people by altering the composition of gut microbiota through antimicrobial activity. The finding that parabens exert dual toxicological effects on sperm quality provides novel insights for future research.
TP53 mutation–associated and copy number–dependent KDM7A-DT expression affects DNA repair and promotes invasive breast cancer progression
Authors: Giannakakis Antonis , Tsifintaris Margaritis , Gouzouasis Vasilis , Tafyllou Charisiostrian , Ow Ghim Siong , Papp Csaba , Ivshina Anna , kuznetsov vladimir
Published: August 2022

Background: Recent characterisation of stress-induced promoter-associated antisense lncRNAs (si-paancRNAs) suggests that these lncRNAs modulate transcription, cellular responses to oxidative, metabolic and genotoxic stress, and may be part of the pathways critical in cancer. KDM7A divergent transcribed (KDM7A-DT) is among the top overexpressed genes encoded by such lncRNAs. The mechanisms leading to aberrant KDM7A-DT transcription, transcript biogenesis, and downstream functions of KDM7A-DT isoforms in breast cancer (BC) types and subtypes have not been studied. Methods: Cell line models, biochemistry methods and microarrays experiments for overexpression and knockdown of KDM7A-DT were used. Next, integration of experimental models, bioinformatics, and massive BRCA patient metadata analyses were performed to investigate the mechanisms and functions of KDM7A-DT.Results: The stable KDM7A-DT overexpression in non-malignant cells upregulates p53, CDKN1A, and γH2AX signalling, resulting in a prolonged cell growth retardation phenotype and preferential depletion of epithelial-to-mesenchymal transition (EMT) pathway–associated tumour suppressor genes. Importantly, KDM7A-DT induction by acute oxidative stress of semi-transformed fibroblasts is p53-dependent. According to BC clinical metadata, KDM7A-DT is overexpressed in poor survival basal-like BC (BLBC) subtype. The KDM7A-DT overexpression is associated with the overexpression of the TP53-missense mutated gene and KDM7A-DT full-length copy number amplification. KDM7A-DT overexpression affects DNA repair via the non-homologous end-joining (NHEJ) pathway, inhibits tumor suppressors involved in EMT, induces pro-oncogenic metabolic changes, and correlates with histologic grades, aneuploidy, hypoxia, cancer-associated proteins, clinical and molecular markers.Conclusion: KDM7A-DT is the TP53 mutation–associated and copy number–dependent pro-oncogene si-paancRNAs that contributes to genome instability, initiation, progression, invasiveness, and BLBC outcomes.
Thermal conductivity of binary Platinum-Iridium alloys
Authors: Sun Hongsheng , Chen Haoran , Chen Yu , Wei Chaoqun , Wang Qing , Gan Youyu , Qi Yanwu , Wang Jianli
Published: August 2022

Measurements of thermal conductivity and electrical conductivity of Platinum-Iridium alloys at different compositions are performed. The thermal conductivities measured by the electrical-heating method and the cross-wire geometry agree well with each other. For the binary alloys with Ir compositions of 6 at.% to 15 at.%, the thermal conductivity varies linearly with temperature, which can be related to the electrical counterpart by using the classical Simth-Palmer formula. The lattice component of the thermal conductivity is found to be nearly composition-independent, contributing to about 20~25% of the total thermal conductivity.
Identification of 7 key genes as biomarkers for prognosis and metastasis of gastric cancer
Authors: Fan Shuyuan , Qiu Feng , Liu jing , Li bin , Li Kun , Diao yunpeng
Published: August 2022

Background
Gastric cancer is one of the most common tumors in the GI system worldwide. The refractory nature of gastric cancer and the increasing risk of recurrence and metastasis with inadequate treatment have led to numerous reports of death due to recurrence and metastasis of gastric cancer. In turn, potential mechanisms and potential biomarkers for the prevention of cancer metastasis have become a major task in clinical cancer research.
Methods
Differential genes (DEGs) up- and down-regulated in the GEO dataset (GSE118897) were identified, and 145 gastric cancer patients from the TCGA database were evaluated for differential gene prognosis and the impact of high expression of these genes in gastric cancer tissues on survival of gastric cancer patients was explored.
Results
A total of 90 DEGs were identified, of which 33 were expressed up-regulated and 57 were expressed down-regulated. By screening genes that were highly expressed in tumor tissues and had poor prognosis for gastric cancer patients, seven key genes were finally screened, including CEACAM7, THBS2, TREM2, MFAP2, BGN, MMP1 and NUSAP1. The expression levels of these genes were shown to correlate with overall survival by survival analysis. These genes are involved in biological processes such as cell proliferation and division, while being reflected in the KEGG-enriched pathway with a higher cell cycle and inflammatory response. Further analysis revealed that all seven genes can affect the proliferation and metastasis of cancer cells, with significant correlation with increasing expression, suggesting a possible significant implication for the prevention and targeted treatment of gastric cancer.
Two-fluid numerical model of chromospheric1heating and plasma outflows in a quiet-Sun
Authors: Murawski Kris , Musielak Zdzislaw , Poedts Stefaan , Srivast Abhishek
Published: August 2022

Purpose: This paper addresses long-standing solar physics problems, namely, the heating of the solar chromosphere and the origin of the solar wind. Our aim is to reveal the related mechanisms behind chromospheric heating and plasma outflows in a quiet-Sun. Methods: The approach is based on a two-fluid numerical model that accounts for thermal non-equilibrium (ionization/recombination), non-adiabatic and non-ideal dynamics of protons+electrons and hydrogen atoms. The model is applied to numerically simulate the propagation and dissipation of granulation-generated waves in the chromosphere and plasma flows inside a quiet region. Results: The obtained results demonstrate that collisions between protons+electrons and hydrogen atoms supplemented by plasma viscosity, magnetic resistivity, and recombination lead to thermal energy release, which compensates radiative and thermal losses in the chromosphere, and sustains the atmosphere with vertical profiles of averaged temperature and periods of generated waves that are consistent with recent observational data. Conclusion: Our model conjectures a most robust and global physical picture of granulation generated wave motions, plasma flows, and subsequent heating, which form and dynamically couple the various layers of the solar atmosphere.
Precise solutions to the Bogoyavlenskii equation byextended rational techniques
Authors: Karchi Nikan Ahmadi , Ghaemi Mohammad Bagher , Vahidi Javad
Published: August 2022

In this article, after implementing traveling wave solutions on the Bogoyavlenskii equation ,and then by employing the extended rational methods, we reachsolutions. One of the applicability ways to find the solutions of nonlinear evolution equations are proposed in this paper.
Effect of timing of bronchodilator therapy initiation on exacerbations in patients with chronic obstructive pulmonary disease: a retrospective cohort study
Authors: Yamada Hideyasu , Matsumoto Isao , Makita Naoyuki , Arita Yoshifumi , Hayashi Nobuya , Mitsuoka Kurena , Tashiro Naoki , Hizawa Nobuyuki
Published: August 2022

Background: The benefit of prompt vs delayed treatment initiation with inhaled long-acting bronchodilators in reducing exacerbations in chronic obstructive pulmonary disease (COPD) is unclear. This study aimed to investigate if long-acting bronchodilator therapy initiation within 30 days of COPD diagnosis reduces exacerbation risk in patients with COPD. Methods: This was a retrospective cohort study of patients with COPD based on claims and electronic medical records data extracted from the Real World Data database. The index date (day 0) was the date of the first confirmed inpatient or outpatient COPD diagnosis between January 1, 2005, and December 31, 2018. Patients with COPD without an asthma diagnosis and aged ≥40 years at the index date were included. Patients who initiated inhaled long-acting bronchodilator therapy within the first 30 days (day 0 to day 29) were categorized into the “prompt therapy” group and the rest into the “delayed therapy” group. Time from day 30 post-diagnosis to the first exacerbation and annual exacerbation rate (AER) were evaluated for the overall population and those stratified by COPD phenotype, including chronic bronchitis (CB) and emphysema. Results: Compared with the delayed therapy group (n=1,516), time to first exacerbation was prolonged (hazard ratio 0.78; 95% confidence interval [CI] [0.70, 0.87]) and annual rates of moderate or severe exacerbations were lower (rate ratio 0.74; 95% CI [0.65, 0.84]) in the prompt therapy group (n=1,466). Similarly, time to first exacerbation was prolonged and AERs were lower in the prompt therapy group in the subgroups of patients with CB or emphysema. Conclusions: This is the first study to demonstrate a prolonged time to first exacerbation upon initiation of long-acting bronchodilators within 30 days of COPD diagnosis. A beneficial effect was also observed in patients with CB and emphysema. Our data support advising patients to initiate long‑acting bronchodilators soon after COPD diagnosis.
Maximum, Minimum and Daily Temperature Range Trends as an Indication of Global Climate Change; Beer Sheva Israel
Authors: Kudish Avraham I , Evseev Efim G
Published: August 2022

An in-depth trend analysis of a database consisting of dry bulb temperatures measured at Beer Sheva, Israel during the time interval between August 1988 and December 2021 is presented. Beer Sheva is located at the northern edge of the semi-arid Negev region of Israel. Trend analyses were applied first to the mean diurnal (06:00-18:00), nocturnal (18:00-06:00) and daily (24 hour) time intervals. Positive trends were detected for all months of the year for both diurnal and nocturnal time intervals with the exception of April and December. The positive trends were in the range of 1.13 and 0.33°C/decade. The magnitude of the positive trends for the nocturnal time interval were greater than that of the diurnal for six months of the year and essentially equal during the month of November. In the case of the daily time interval positive trends were found throughout the year with the exception of April. The positive trends were in the range of 1.05 and 0.41°C/decade. The second phase of the study was a trend analyses performed on the daily maximum, daily minimum and Daily Temperature Range (DTR). The daily maximum temperature exhibited positive trends only during January, February, March, July and August, whereas the daily minimum temperature trends were positive throughout the year. In addition, only during January and February were the maximum daily temperatures greater that the corresponding minimum temperatures; during March there were essentially the same. The DTR was found to possess negative trends from May through October. The highest negative trend, -0.96°C/decade, is in June and the lowest negative trend, -0.48°C/decade, occurs in October.
Prevalence of adverse events varies with the different oral isotretinoin brands in acne treatment: a retrospective observational study.
Authors: Soutou Boutros , Sleiman Joelle , Tomb Roland , Kechichian Elio , Helou Josiane
Published: August 2022

Oral isotretinoin remains the most effective treatment for acne. The aim of this retrospective single-center cohort study was to estimate the prevalence of adverse events with the different oral isotretinoin brands used in acne treatment. The population consisted of all patients who consulted for acne between January 2015 and January 2020. The inclusion criterion was the initiation of treatment with oral isotretinoin. The exclusion criteria were the use of two or more brands during the same course of treatment and previous treatment with oral isotretinoin. Statistical analysis was carried out using Chi-square and Mann-Whitney tests. We analyzed 468 patients of whom 68.6% were female. The median age was 21 years. The median weight was 65 kg. The treatment was Roaccutane®, Curacné®, Acnotren®, Isosupra®, Contracné®, or Acnogen® in 44.2%, 28%, 14.5%, 10.5%, 1.7% and 0.4% of cases, respectively. Xerosis was the most frequently reported side effect regardless of the brand. The highest frequencies of: - hypercholesterolemia (25.6%) and eczema (13%) were noted with Roaccutane®; - hypertriglyceridemia (16.8%), epistaxis (9.9%) and fatigue (3.1%) with Curacné®; - excessive sweating (4.1%) and headache (4.1%) with Isosupra®; - abnormal liver function tests (11%) with Acnotren®. We found a significant correlation mainly between abnormal ASAT and Acnotren®(p = 0.009), hypercholesterolemia and Roaccutane® [OR = 1.652 (95%CI: 1.056–2.585)], hypertriglyceridemia and higher body weight (p = 0.004). Factors related to the drug brand and to characteristics of acne patients could explain the variability in the prevalence of some adverse events.
Pancreaticoduodenectomy with celiac artery resection (PD-CAR) for locally advanced pancreatic ductal adenocarcinoma Name of the authors
Authors: Kimura Kenjiro , Amano Ryosuke , Tauchi Jun , Nishio Kohei , Ohira Go , Shinkawa Hiroji , Tanaka Shogo , Yamamoto Akira , Motomura Hisashi , Ishizawa Takeaki
Published: August 2022

Purpose
Locally advanced pancreatic ductal adenocarcinoma (LA-PDAC) involving the celiac artery (CeA), the common hepatic artery and the gastroduodenal artery (GDA) is considered unresectable. We developed the novel procedure of pancreaticoduodenectomy with celiac artery resection (PD-CAR) for such LA-PDACs.
Methods
From 2015 to 2018, we performed curative pancreatectomy with major arterial resection for 13 LA-PDACs as a clinical study (UMIN000029501). Of those, four patients with pancreatic neck cancer involving the CeA and GDA were candidates for PD-CAR. Prior to surgery, blood flow alterations were performed to unify the blood flow to the liver, stomach, and pancreas, resulting in feeding from the cancer-free artery. During PD-CAR, arterial reconstruction of the unified artery was performed as needed. Based on the records of PD-CAR cases, we retrospectively analyzed the validity of the operation.
Results
R0 resection was achieved in all patients. Arterial reconstruction was performed in three patients. In another patient, the hepatic arterial flow was maintained by preserving of the left gastric artery. The mean operative time was 669 min and the mean blood loss was 1003 g. Although Clavien-Dindo classification III-IV postoperative morbidities occurred in three patients, no reoperations nor mortalities occurred. Although two patients died of cancer recurrence, one patient survived for 26 months without recurrence (died of cerebral infarction), and another is alive at 76 months without recurrence.
Conclusion
PD-CAR, which enabled R0 resection and preservation of the residual stomach, pancreas, and spleen, provided acceptable postoperative outcomes.
Early Mitochondrial Defects in the 5xFAD mouse model of Alzheimer’s Disease
Authors: Sharma Neelam , Banerjee Rupkatha , Davis Ronald L.
Published: August 2022

BACKGROUND
Mitochondrial (MT) dysfunction is a hallmark of Alzheimer’s disease (AD). Amyloid Precursor Protein and Aβ peptides localize to MT and lead to MT dysfunction in familial forms of AD. This dysfunction may trigger subsequent types of pathology. Identification of the MT phenotypes that occur early is required to help understand the cascade of AD pathophysiology.
METHODS
The 5xFAD mouse model was used to explore the time course of MT pathologies in both sexes. Protein biomarkers for MT dynamics were measured biochemically and MT function was measured using oxygen consumption and ATP assays.
RESULTS
We discovered progressive alterations in mitochondrial dynamics (biogenesis, fission, fusion, and mitophagy) and function (O2 consumption, ATP generation, and Ca2+ import) in the hippocampus of 5xFAD mice in both sexes as early as 2 month-of-age. Thus, mitochondrial dynamics and function become altered at young ages, consistent with an early role for mitochondria in the AD pathological cascade.
CONCLUSIONS
Our study offers the baseline information required to understand the hierarchical relationship between the multiple pathologies that develop in this mouse model and provides early biomarkers for MT dysfunction. This will aid in dissecting the temporal cascade of pathologies, understanding sex-specific differences, and in testing the efficacy of putative mitochondrial therapeutics.
Blockchain in Financial Services: Current Status, Adaptation Challenges, and Future Vision
Authors: Mia Anisha , Rahouti Mohamed , Jagatheesaperumal Senthil Kumar , Ayyash Moussa , Xiong Kaiqi , Fernandez Fredery , Lekena Modupe
Published: August 2022

Blockchain is undoubtedly considered one of the most innovative technologies in financial services from the past decade. Interests in blockchain technology continue to grow on a daily basis, while many promising blockchain-enabled applications and services continue to draw financial interests in the industrial sector and the broader financial services communities. Furthermore, as blockchain matures, it increases the value of a diversified range of industries and institutes with a better return on investment. However, such organizations must continue to adopt rigorous approaches to enhance their financial services and regulate their policies. In this article, we discuss the current status of blockchain-enabled financial services and applications, in addition to the impacts, implications, and regulations of incorporating blockchain technology in the finance industry. We further tackle the adaptation model for blockchain technology in global banking and provide a vision for the next generation of financial services based on this emerging technology.