bluuebunny/crossref_metadata_2025 huggingface dataset:
The Cross-Level Moderation Effect of Resource-Providing Leadership on the Demands—Work Ability Relationship
Authors: Richter Anne , Roczniewska Marta , Loeb Carina , Stempel Christiane R. , Rigotti Thomas
Published: August 2021

Employees in female-dominated sectors are exposed to high workloads, emotional job demands, and role ambiguity, and often have insufficient resources to deal with these demands. This imbalance causes strain, threatening employees’ work ability. The aim of this study was to examine whether resource-providing leadership at the workplace level buffers against the negative repercussions of these job demands on work ability. Employees (N = 2383) from 290 work groups across three countries (Germany, Finland, and Sweden) in female-dominated sectors were asked to complete questionnaires in this study. Employees rated their immediate supervisor’s resource-providing leadership and also self-reported their work ability, role ambiguity, workload, and emotional demands. Multilevel modeling was performed to predict individual work ability with job demands as employee-level predictors, and leadership as a group-level predictor. Work ability was poor when employees reported high workloads, high role ambiguity, and high emotional demands. Resource-providing leadership at the group level had a positive impact on employees’ work ability. We observed a cross-level interaction between emotional demands and resource-providing leadership. We conclude that resource-providing leadership buffers against the repercussions of emotional demands for the work ability of employees in female-dominated sectors; however, it is not influential in dealing with workload or role ambiguity.
Does Climate Play Any Role in COVID-19 Spreading?—An Australian Perspective
Authors: Abraham Joji , Turville Christopher , Dowling Kim , Florentine Singarayer
Published: August 2021

Compared to other countries, the COVID-19 pandemic did not severely affect Australia as measured by total deaths until mid-2021. Though a substantial number of daily confirmed cases (up to 698) were reported during the second wave, most of them were from the southern state of Victoria. This study examined the possible correlations between climate variables and the number of daily confirmed COVID-19 cases in Victoria, Australia, from 25 January to 31 October 2020. Appropriate regression models and cross-correlation diagnostics were used to examine the effect of temperature, rainfall, solar exposure, and ultraviolet index (UVI) with the number of daily confirmed cases. Significant positive associations were identified for solar exposure and maximum and average UVI for confirmed cases one and 19 days later. Negative associations for these variables were found for confirmed cases five days later. Minimum temperature had a significant negative correlation one day later and a positive effect 21 days later. No significant correlation was found for maximum temperature and rainfall. The most significant relationships were found for confirmed cases 19 days after changes in the meteorological variables. A 1% increase in solar exposure, maximum UVI, and average UVI was associated with a 0.31% (95% CI: 0.13 to 0.51), 0.71% (95% CI: 0.43 to 0.98), and 0.63% (95%CI: 0.20 to 1.61) increase 19 days later in the number of confirmed cases, respectively. The implications of these results can be used in the public health management of any possible future events in Australia. It also highlights the significance of considering the climatic variables and seasonality in all kinds of epidemics and pandemics.
Balanced Convolutional Neural Networks for Pneumoconiosis Detection
Authors: Hao Chaofan , Jin Nan , Qiu Cuijuan , Ba Kun , Wang Xiaoxi , Zhang Huadong , Zhao Qi , Huang Biqing
Published: August 2021

Pneumoconiosis remains one of the most common and harmful occupational diseases in China, leading to huge economic losses to society with its high prevalence and costly treatment. Diagnosis of pneumoconiosis still strongly depends on the experience of radiologists, which affects rapid detection on large populations. Recent research focuses on computer-aided detection based on machine learning. These have achieved high accuracy, among which artificial neural network (ANN) shows excellent performance. However, due to imbalanced samples and lack of interpretability, wide utilization in clinical practice meets difficulty. To address these problems, we first establish a pneumoconiosis radiograph dataset, including both positive and negative samples. Second, deep convolutional diagnosis approaches are compared in pneumoconiosis detection, and a balanced training is adopted to promote recall. Comprehensive experiments conducted on this dataset demonstrate high accuracy (88.6%). Third, we explain diagnosis results by visualizing suspected opacities on pneumoconiosis radiographs, which could provide solid diagnostic reference for surgeons.
HIA and EIA Are Different, but Maybe Not in the Way We Thought They Were: A Bibliometric Analysis
Authors: Kim Jinhee , Haigh Fiona Anne
Published: August 2021

Background: The fields of Health Impact Assessment (HIA) and Environmental Impact Assessment (EIA) have grown with increasing numbers of disciplines and sectors contributing to their advancements, but with it, perceived conflict over methodological and disciplinary approaches to integrate health in impact assessments. This study maps the current field of HIA and health in EIA to examine the scientific landscape of the field. Methods: We carried out a bibliometric analysis of HIA papers and EIA papers that included a health focus in peer-reviewed journals in the Web of Science Core Collection (n = 229). We carried out co-authorship and co-citation network analyses of authors and documents in VOSviewer. Results: We identified two main co-authorship and co-citation groupings. Our document co-citation analysis also identified four clusters with two major groups, the Defining HIA cluster and the Describing the fields cluster versus the Active transport quantitative HIA cluster, and the Quantitative modelling tools cluster. Conclusion: Our findings strongly suggest that there exist two groups of thought in the scholarly fields of HIA and health in EIA. Barriers to developing more methodologically integrated approaches to considering health within EIA are related more to disciplinary differences than field (HIA versus EIA)-based differences and we advocate for the development of transdisciplinary approaches to both HIA and EIA.
Increased Use of Porch or Backyard Nature during COVID-19 Associated with Lower Stress and Better Symptom Experience among Breast Cancer Patients
Authors: Pearson Amber L. , Breeze Victoria , Reuben Aaron , Wyatt Gwen
Published: August 2021

Contact with nature has been used to promote both physical and mental health, and is increasingly used among cancer patients. However, the COVID-19 pandemic created new challenges in both access to nature in public spaces and in cancer care. The purpose of our study was to evaluate the change in active and passive use of nature, places of engaging with nature and associations of nature contact with respect to improvements to perceived stress and symptom experience among breast cancer patients during the pandemic. We conducted a cross-sectional survey of people diagnosed with breast cancer using ResearchMatch (n = 56) in July 2020 (the first wave of COVID-19). In this US-based, predominantly white, affluent, highly educated, female sample, we found that, on average, participants were first diagnosed with breast cancer at 54 years old and at stage 2 or 3. Eighteen percent of participants experienced disruptions in their cancer care due to the pandemic. As expected, activities in public places significantly decreased as well, including use of parks/trails and botanical gardens. In contrast, spending time near home, on the porch or in the backyard significantly increased. Also observed were significant increases in indoor activities involving passive nature contact, such as watching birds through a window, listening to birdsong, and smelling rain or plants. Decreased usage of parks/trails was significantly associated with higher stress (Coef = −2.30, p = 0.030) and increased usage of the backyard/porch was significantly associated with lower stress (Coef = −2.69, p = 0.032), lower symptom distress (Coef = −0.80, p = 0.063) and lower symptom severity (Coef = −0.52, p = 0.009). The most commonly reported alternatives to outdoor engagement with nature were watching nature through a window (84%), followed by looking at images of nature (71%), and listening to nature through a window (66%). The least commonly enjoyed alternative was virtual reality of nature scenes (25%). While outdoor contact with nature away from home decreased, participants still found ways to experience the restorative benefits of nature in and around their home. Of special interest in planning interventions was the fact that actual or real nature was preferred over that experienced through technology. This could be an artifact of our sample, or could represent a desire to be in touch with the “real world” during a health crisis. Nature contact may represent a flexible strategy to decrease stress and improve symptom experience among patients with cancer, particularly during public health crises or disruptions to cancer care.
A Comprehensive Community-Based Prevalence Study on Nocturia in Hong Kong Male Adults
Authors: Yuen John Wai-Man , Wong Ivy Yuen-Ping , Chiu Peter Ka-Fung , Teoh Jeremy Yuen-Chun , Chan Chi-Kwok , Yee Chi-Hang , Ng Chi-Fai
Published: August 2021

Background: Most prevalence surveys on nocturia have focused on older populations. This study aimed to measure the nocturia prevalence across the full spectrum of male adults living in Hong Kong, where severity and associated quality-of-life (QoL) were also explored. Methods: A cross-sectional population-based survey was conducted in men aged 18 or above using the ICIQ-NQoL Questionnaire. Results: With 1239 respondents at age ranged 18–99, the overall nocturia prevalences were found to be 63.0% (ranged 41.6–84.6% at different age groups) and 31.2% (ranged 13.0–56.3% at different age groups), for ≥1 and ≥2 bedtime voiding episodes, respectively. The chance of nocturia was dramatically increased at age 60 or above while both prevalence and voiding frequency were increased with advancing age. About 83% of the nocturia men experienced one to two voiding episodes per night, but many of them had self-rated their QoL poor or very poor and indicated moderate-to-high bothersome. Younger men at age 18–39 were found to have high prevalence as 41.6% and almost 30% of them rated poor or very poor QoL. Conclusions: Nocturia was not only affecting the older populations but also caused significant bothersome and negative impacts on QoL in younger males.
The Effect of Functional Motivation on Future Intention to Donate Blood: Moderating Role of the Blood Donor’s Stage
Authors: Zhang Lifan , Yao Meilin , Liu Hongrui , Zheng Shuang
Published: August 2021

The low retention rate and lack of long-term blood donors are still worldwide problems, and the fostering and retaining of more blood donors has posed an urgent problem that requires a solution. The purpose of this study was to explore the role of functional motivation and blood donor stage in the future intention to donate blood. Three sites in China provided 252 blood donors who filled out a questionnaire including the volunteer function inventory, their future intention to donate blood, number of blood donations and demographic information. Based on a functional motivation approach, we investigated the motivations that influence unpaid blood donors’ future intention through qualitative interview methods. The results showed that blood donor stage moderated the relationship between values motivation and future intention to donate blood. More specifically, the predictive effect of values motivation on future intention decreased gradually from new donors to regular donors. Our study found that the effect of various motivations on blood donors’ intention was conditional; this effect changed with the development of blood donor behavioral stages. The results are significant in practice for establishing effective intervene strategies in maintaining blood donors.
Comparison of Experiences in Two Birth Cohorts Comprising Young Families with Children under Four Years during the Initial COVID-19 Lockdown in Australia and the UK: A Qualitative Study
Authors: Gibson Lisa Y. , Lockyer Bridget , Dickerson Josie , Endacott Charlotte , Bridges Sally , McEachan Rosemary R. C. , Pickett Kate E. , Whalan Sarah , Bear Natasha L. , Silva Desiree T. , Prescott Susan L. , Davis Jacqueline A.
Published: August 2021

This study aims to understand the experience and impact of the initial COVID-19 lockdown in young families with children aged below 4 years. Free text questions were administered to participants in the ORIGINS (Australia) and Born in Bradford (UK) cohort studies to collect qualitative information on worries, concerns and enjoyable experiences during the pandemic. A total of 903 (400 for ORIGINS and 503 for BiB) participants completed the two surveys during April 2020. Despite varying in geography, levels of socio-economic disadvantage and their situational context during the pandemic, respondents from both cohorts reported similar worries and challenges during the lockdown period, including: employment/finances, health anxiety, mental health and social isolation, caring for children and child development. Families across the globe experienced both positive and negative immediate impacts of COVID-19. Population-based data can be used to inform the development of support services, public health campaigns and universal interventions to assist families in future health crises.
Regional Effects of Perceived Risks of Harm on Cigarette Smoking among U.S. High School Seniors: Evidence from Monitoring the Future
Authors: Medina Jorge
Published: August 2021

Overall, there has been an increasing trend in the perceived risk of harm from smoking among U.S. high school seniors. However, these perceptions of risk have been falling in recent years. This study uses regional-level panel data from the Monitoring the Future survey and a fixed effects model to estimate the effect of perceived risk on three regional measurements of smoking behavior: consumption, lifetime prevalence, and daily smoking prevalence. Elasticity measurements at regional levels show that an increase in perceived risk decreases these regional measurements of smoking behavior. Moreover, the results show that, at regional levels, these measurements of smoking behavior are more responsive to changes in the perceived risk associated with smoking than to changes in the price of cigarettes.
Current Issues within the Perinatal Mental Health Care System in Aichi Prefecture, Japan: A Cross-Sectional Questionnaire Survey
Authors: Fujita Kei , Kotani Tomomi , Moriyama Yoshinori , Ushida Takafumi , Imai Kenji , Kobayashi-Nakano Tomoko , Kato Noriko , Kano Takeo , Kikkawa Fumitaka , Kajiyama Hiroaki
Published: August 2021

Mental illnesses commonly occur in the reproductive age. This study aimed to identify the issues that exist within the perinatal mental health care system. A cross-sectional survey was conducted in Aichi Prefecture in central Japan. Questionnaires on the situation between 2016 and 2018 were mailed to the head physicians of 128 maternity care units, 21 neonatal intensive care units (NICUs), and 40 assisted reproductive technology (ART) units. A total of 82 (52.6 per 100,000 births) women were admitted to mental health care units during the perinatal period, and 158 (1.0 per 1000 births) neonates born to mothers with mental illness were admitted to NICUs. Approximately 40% of patients were hospitalized in psychiatric hospitals without maternity care units. Eighty-four (71.1%) and 76 (64.4%) maternity care units did not have psychiatrists or social workers, respectively. Moreover, 20–35% of the head physicians in private clinics, general hospitals, and ART units endorsed the discontinuation of psychotropic drug use during pregnancy. However, the corresponding figures were only 5% among those in maternal-fetal centers. Resources for perinatal mental illness might be limited. Perspectives on psychotropic drug use differed based on the type of facilities where the doctors were working.
Impact of Substantive Staging and Communicative Staging of Sustainable Servicescape on Behavioral Intentions of Hotel Customers through Overall Perceived Image: A Case of Boutique Hotels
Authors: Khalil-ur-Rehman  , Adnan Mohammad , Ahmad Naveed , Scholz Miklas , Khalique Muhammad , Naveed Rana Tahir , Han Heesup
Published: August 2021

Customers have become very sensitive regarding the innovative evaluation of services. Due to competition in the hospitality industry, it is a challenge for hotel marketers to understand customers’ behavior. There is scant research in the hotel industry of Pakistan and especially on boutique hotels. This research seeks to measure the relationship between substantive, communicative elements of the sustainable servicescape and behavioral intentions (word of mouth) in a boutique hotel setting. However, the mediating effect of the overall perceived image is examined between these constructs. Responses of boutique hotel visitors were collected from Lahore, Islamabad, Faisalabad, and Murree. Data were analyzed by using structural equation modeling (SEM). Results display that both substantive and communicative servicescape elements positively affect the perceived image of customers, which has a positive influence on behavioral intentions such as word of mouth (WOM). Theoretical and practical implications are also discussed.
Gender Differences in Depressive Traits among Rural and Urban Chinese Adolescent Students: Secondary Data Analysis of Nationwide Survey CFPS
Authors: Chen Weilong , Huang Yi , Riad Abanoub
Published: August 2021

Many previous studies have indicated that urban adolescents show a higher level of mental health in China compared to rural adolescents. Specifically, girls in rural areas represented a high-risk group prior to the 21st century, demonstrating more suicidal behaviour and ideation than those in the urban areas because of the severe gender inequality in rural China. However, because of the urbanisation process and centralised policy to eliminate gender inequality in recent decades, the regional and gender differences in mental health might decrease. This research aimed to probe the gender and regional differences in depressive traits among adolescent students currently in China. We adopted the national survey dataset Chinese Family Panel Studies (CFPS) conducted in 2018. Accordingly, 2173 observations from 10–15-year-old subjects were included. CFPS utilised an eight-item questionnaire to screen individuals’ depressive traits. Two dimensions of depressive traits were confirmed by CFA, namely depressed affect and anhedonia. The measurement invariance tests suggested that the two-factor model was applicable for both males and females and rural and urban students. Based on the extracted values from the CFA model, MANOVA results revealed that, compared to boys, girls experienced more depressed affect. Moreover, rural students demonstrated more anhedonia symptoms. There was no interaction between gender and region. The results suggest that, even though the gender and regional differences are small, being a female and coming from a rural area are still potential risk factors for developing depressive traits among adolescent students in China.
One Year on: An Overview of Singapore’s Response to COVID-19—What We Did, How We Fared, How We Can Move Forward
Authors: Anand S Vivek , Shuy Yao Kang , Lee Poay Sian Sabrina , Lee Eng Sing
Published: August 2021

Background—One year has passed since the first COVID-19 case in Singapore. This scoping review commemorates Singaporean researchers that have expanded the knowledge on this novel virus. We aim to provide an overview of healthcare-related articles published in peer-reviewed journals, authored by the Singapore research community about COVID-19 during the first year of the pandemic. Methods—This was reported using the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) protocol. It included healthcare-related articles about COVID-19 published between 23 January 2020 and 22 January 2021 with a Singapore-affiliated author. MEDLINE, Embase, Scopus, Web of Science, CINAHL, PsycINFO, Google Scholar, and local journals were searched. The articles were screened independently by two reviewers. Results—The review included 504 articles. Most of the articles narrated the changes to hospital practice (210), while articles on COVID-19 pathology (94) formed most of the non-narrative papers. Publications on public health (61) and the indirect impacts to clinical outcomes (45) were other major themes explored by the research community. The remaining articles detailed the psychological impact of the pandemic (35), adaptations of medical education (30), and narratives of events (14). Conclusion—Amidst a resurgence of community cases involving variant COVID-19 strains, the resources from the research community will provide valuable guidance to navigate these uncertain times.
Intergenerational Association of Short Maternal Stature with Stunting in Yanomami Indigenous Children from the Brazilian Amazon
Authors: Orellana Jesem Douglas Yamall , Gatica-Domínguez Giovanna , Vaz Juliana dos Santos , Neves Paulo Augusto Ribeiro , de Vasconcellos Ana Claudia Santiago , de Souza Hacon Sandra , Basta Paulo Cesar
Published: August 2021

To describe the factors associated to stunting in <5-year-old Yanomami Brazilian children, and to evaluate the association of short maternal stature to their offspring’s stunting. A cross-sectional study carried out in three villages in the Yanomami territory. We performed a census, in which all households with children < 5-years-old were included. The length/height-for-age z-score <−2 standard deviations was used to classify the children as stunted. Short maternal height was defined as <145 cm for adult women, and <−2 standard deviations of the height-for-age z-score for adolescent women. We used adjusted Poisson regression models to estimate prevalence ratios (PR) along the 90% confidence interval. We evaluated 298 children. 81.2% of children suffered from stunting and 71.9% of the mothers from short stature. In the bivariate analysis, a significant association of stunting with short maternal stature, gestational malaria and child’s place of birth were observed. Considering the variables of the children under five years of age, there were significant associations with age group, the child’s caregiver, history of malaria, pneumonia, and malnutrition treatment. In the adjusted hierarchical model, stunting was 1.22 times greater in the offspring of women with a short stature (90% CI: 1.07–1.38) compared to their counterparts. Brazilian Amazonian indigenous children living in a remote area displayed an alarming prevalence of stunting, and this was associated with short maternal height, reinforcing the hypothesis of intergenerational chronic malnutrition transmission in this population. In addition, children above 24 months of age, who were born in the village healthcare units and who had had previous treatment in the past for stunting presented higher rates of stunting in this study.
Effects of Exposure to Road, Railway, Airport and Recreational Noise on Blood Pressure and Hypertension
Authors: Petri Davide , Licitra Gaetano , Vigotti Maria Angela , Fredianelli Luca
Published: August 2021

Noise is one of the most diffused environmental stressors affecting modern life. As such, the scientific community is committed to studying the main emission and transmission mechanisms aiming at reducing citizens’ exposure, but is also actively studying the effects that noise has on health. However, scientific literature lacks data on multiple sources of noise and cardiovascular outcomes. The present cross-sectional study aims to evaluate the impact that different types of noise source (road, railway, airport and recreational) in an urban context have on blood pressure variations and hypertension. 517 citizens of Pisa, Italy, were subjected to a structured questionnaire and five measures of blood pressure in one day. Participants were living in the same building for at least 5 years, were aged from 37 to 72 years old and were exposed to one or more noise sources among air traffic, road traffic, railway and recreational noise. Logistic and multivariate linear regression models have been applied in order to assess the association between exposures and health outcomes. The analyses showed that prevalence of high levels of diastolic blood pressure (DBP) is consistent with an increase of 5 dB (A) of night-time noise (β = 0.50 95% CI: 0.18–0.81). Furthermore, increased DBP is also positively associated with more noise sensitive subjects, older than 65 years old, without domestic noise protection, or who never close windows. Among the various noise sources, railway noise was found to be the most associated with DBP (β = 0.68; 95% CI: −1.36, 2.72). The obtained relation between DBP and night-time noise levels reinforces current knowledge.
Systematic Review and Meta-Analysis of Tocilizumab Therapy versus Standard of Care in over 15,000 COVID-19 Pneumonia Patients during the First Eight Months of the Pandemic
Authors: Mahroum Naim , Watad Abdulla , Bridgewood Charlie , Mansour Muhammad , Nasr Ahmad , Hussein Amr , Khamisy-Farah Rola , Farah Raymond , Gendelman Omer , Lidar Merav , Shoenfeld Yehuda , Amital Howard , Kong Jude Dzevela , Wu Jianhong , Bragazzi Nicola Luigi , McGonagle Dennis
Published: August 2021

Background. Tocilizumab is an anti-IL-6 therapy widely adopted in the management of the so-called “cytokine storm” related to SARS-CoV-2 virus infection, but its effectiveness, use in relation to concomitant corticosteroid therapy and safety were unproven despite widespread use in numerous studies, mostly open label at the start of the pandemic. Methods: We performed a systematic review and meta-analysis of case-control studies utilising tocilizumab in COVID-19 on different databases (PubMed/MEDLINE/Scopus) and preprint servers (medRxiv and SSRN) from inception until 20 July 2020 (PROSPERO CRD42020195690). Subgroup analyses and meta-regressions were performed. The impact of tocilizumab and concomitant corticosteroid therapy or tocilizumab alone versus standard of care (SOC) on the death rate, need for mechanical ventilation, ICU admission and bacterial infections were assessed. Results. Thirty-nine studies with 15,531 patients (3657 cases versus 11,874 controls) were identified. Unadjusted estimates (n = 28) failed to demonstrate a protective effect of tocilizumab on survival (OR 0.74 ([95%CI 0.55–1.01], p = 0.057), mechanical ventilation prevention (OR 2.21 [95%CI 0.53–9.23], p = 0.277) or prevention of ICU admission (OR 3.79 [95%CI 0.38–37.34], p = 0.254). Considering studies with adjusted, estimated, tocilizumab use was associated with mortality rate reduction (HR 0.50 ([95%CI 0.38–0.64], p < 0.001) and prevention of ICU admission (OR 0.16 ([95%CI 0.06–0.43], p < 0.001). Tocilizumab with concomitant steroid use versus SOC was protective with an OR of 0.49 ([95%CI 0.36–0.65], p < 0.05) as was tocilizumab alone versus SOC with an OR of 0.59 ([95%CI 0.34–1.00], p < 0.001). Risk of infection increased (2.36 [95%CI 1.001–5.54], p = 0.050; based on unadjusted estimates). Conclusion: Despite the heterogeneity of included studies and large number of preprint articles, our findings from the first eight of the pandemic in over 15,000 COVID-19 cases suggested an incremental efficacy of tocilizumab in severe COVID-19 that were confirmed by subsequent meta-analyses of large randomized trials of tocilizumab. This suggests that analysis of case-control studies and pre-print server data in the early stages of a pandemic appeared robust for supporting incremental benefits and lack of major therapeutic toxicity of tocilizumab for severe COVID-19.
Benefits and Drawbacks of Ultrasound-Assisted Extraction for the Recovery of Bioactive Compounds from Marine Algae
Authors: Carreira-Casais Anxo , Otero Paz , Garcia-Perez Pascual , Garcia-Oliveira Paula , Pereira Antia G. , Carpena Maria , Soria-Lopez Anton , Simal-Gandara Jesus , Prieto Miguel A.
Published: August 2021

The increase in life expectancy has led to the appearance of chronic diseases and interest in healthy aging, in turn promoting a growing interest in bioactive compounds (BCs) and functional ingredients. There are certain foods or products rich in functional ingredients, and algae are one of them. Algae consumption has been nominal in Europe until now. However, in recent years, it has grown significantly, partly due to globalization and the adoption of new food trends. With the aim of obtaining BCs from foods, multiple methods have been proposed, ranging from conventional ones, such as maceration or Soxhlet extraction, to more innovative methods, e.g., ultrasound-assisted extraction (UAE). UAE constitutes a novel method, belonging to so-called green chemistry, that enables the extraction of BCs requiring lower amounts of solvent and energy costs, preserving the integrity of such molecules. In recent years, this method has been often used for the extraction of different BCs from a wide range of algae, especially polysaccharides, such as carrageenans and alginate; pigments, including fucoxanthin, chlorophylls, or β-carotene; and phenolic compounds, among others. In this way, the application of UAE to marine algae is an efficient and sustainable strategy to pursue their deep characterization as a new source of BCs, especially suitable for vegetarian and vegan diets.
The Asymmetric Pattern of Population Mobility during the Spring Festival in the Yangtze River Delta Based on Complex Network Analysis: An Empirical Analysis of “Tencent Migration” Big Data
Authors: Lin Jinping , Wu Kangmin , Yang Shan , Liu Qianqian
Published: August 2021

Population mobility patterns are an important reflection of the future distribution of migrant populations and the evolution trends of urbanization patterns. However, although research based on statistical data can reveal the pattern of population flow, it also shows a time lag. Most of the population flow network research based on location services data has failed to fully discuss the symmetry of directional outflows and inflows in the same place and the two-way symmetrical connections between places. This paper creatively proposes and constructs the concept and analysis framework of population flow asymmetry. We used the Yangtze River Delta (YRD) as a typical case and the results of our analysis reveal the temporal and spatial asymmetry of the population flow using complex network analysis methods based on the Spring Festival (SF) population migration big data. We found that the timing asymmetry manifested in such a way that the closer it was to the festival, the greater the scale and intensity of the population movement. This is a feature of the lack of scale and regional differences within China. The spatial asymmetry was manifested in three aspects, network, node, and link, and the core cities with administrative and economic hierarchical advantages dominated the asymmetric pattern of regional population mobility. In addition, distance and administrative boundaries are factors that cannot be ignored in population movements, and they were implicated in the degree of asymmetry by distance enhancement and administrative boundary blocking. The conclusions of this study can not only provide policy decision-making guidelines for population management and resource allocation in the YRD, but they can also provide a reference value for achieving the goal of regional, high-quality, integrated development. Future research will further the discussion and management of socio-economic attributes in order to develop a more detailed and microscopic understanding of the mechanisms of population mobility patterns.
Spatiotemporal Characteristics of Urban Land Expansion and Population Growth in Africa from 2001 to 2019: Evidence from Population Density Data
Authors: Jiang Shengnan , Zhang Zhenke , Ren Hang , Wei Guoen , Xu Minghui , Liu Binglin
Published: August 2021

Africa has been undergoing a rapid urbanization process, which is critical to the achievement of the 11th Sustainable Development Goal (SDG11). Using population density data from LandScan, we proposed a population density-based thresholding method to generate urban land and urban population data in Africa from 2001 to 2019, which were further applied to detect the spatiotemporal characteristics of Africa’s urbanization. The results showed that urban land and urban population have both grown rapidly in Africa, which increased by about 5.92% and 4.91%, respectively. The top three countries with the most intense urbanization process in Africa are Nigeria, the Democratic Republic of the Congo, and Ethiopia. The coupling relationship index of urban land expansion and population growth was 0.76 in Africa during 2001–2019. Meanwhile, the total proportion of uncoordinated development types at the provincial level was getting higher, which indicated an uncoordinated relationship between urban land expansion and population growth in Africa. Cropland, grassland, rural land, and forests were the most land-use types occupied by urban expansion. The proportion of cropland, grassland, and forests occupied was getting higher and higher from 2001 to 2019. The extensive urban land use may have an impact on the environmental and economic benefits brought by urbanization, which needs further research.
Probiotic Administration Mitigates Bisphenol A Reproductive Toxicity in Zebrafish
Authors: Giommi Christian , Habibi Hamid R. , Candelma Michela , Carnevali Oliana , Maradonna Francesca
Published: August 2021

Although the use of bisphenol A (BPA) has been banned in a number of countries, its presence in the environment still creates health issues both for humans and wildlife. So far, BPA toxicity has been largely investigated on different biological processes, from reproduction to development, immune system, and metabolism. In zebrafish, Danio rerio, previous studies revealed the ability of environmentally relevant concentrations of this contaminant to significantly impair fertility via epigenetic modification. In addition, several studies demonstrated the ability of different probiotic strains to improve organism health. This study provides information on the role of the probiotic mixture SLAb51 to counteract adverse BPA effects on reproduction. A 28-day trial was set up with different experimental groups: BPA, exposed to 10 µg/L BPA; P, receiving a dietary supplementation of SLAb51 at a final concentration of 109 CFU/g; BPA+P exposed to 10 µg/L BPA and receiving SLAb51 at a final concentration of 109 CFU/g and a C group. Since oocyte growth and maturation represent key aspects for fertility in females, studies were performed on isolated class III (vitellogenic) and IV (in maturation) follicles and liver, with emphasis on the modulation of the different vitellogenin isoforms. In males, key signals regulating spermatogenesis were investigated. Results demonstrated that in fish exposed to the combination of BPA and probiotic, most of the transcripts were closer to C or P levels, supporting the hypothesis of SLAb51 to antagonize BPA toxicity. This study represents the first evidence related to the use of SLAb51 to improve reproduction and open new fields of investigation regarding its use to reduce endocrine disrupting compound impacts on health.
Extracellular Heat Shock Proteins as Therapeutic Targets and Biomarkers in Fibrosing Interstitial Lung Diseases
Authors: Tanguy Julie , Pommerolle Lenny , Garrido Carmen , Kolb Martin , Bonniaud Philippe , Goirand Françoise , Bellaye Pierre-Simon
Published: August 2021

Interstitial lung diseases (ILDs) include a large number of diseases and causes with variable outcomes often associated with progressive fibrosis. Although each of the individual fibrosing ILDs are rare, collectively, they affect a considerable number of patients, representing a significant burden of disease. Idiopathic pulmonary fibrosis (IPF) is the typical chronic fibrosing ILD associated with progressive decline in lung. Other fibrosing ILDs are often associated with connective tissues diseases, including rheumatoid arthritis-ILD (RA-ILD) and systemic sclerosis-associated ILD (SSc-ILD), or environmental/drug exposure. Given the vast number of progressive fibrosing ILDs and the disparities in clinical patterns and disease features, the course of these diseases is heterogeneous and cannot accurately be predicted for an individual patient. As a consequence, the discovery of novel biomarkers for these types of diseases is a major clinical challenge. Heat shock proteins (HSPs) are molecular chaperons that have been extensively described to be involved in fibrogenesis. Their extracellular forms (eHSPs) have been recently and successfully used as therapeutic targets or circulating biomarkers in cancer. The current review will describe the role of eHSPs in fibrosing ILDs, highlighting the importance of these particular stress proteins to develop new therapeutic strategies and discover potential biomarkers in these diseases.
Extracellular Vesicles and Thrombosis: Update on the Clinical and Experimental Evidence
Authors: Zifkos Konstantinos , Dubois Christophe , Schäfer Katrin
Published: August 2021

Extracellular vesicles (EVs) compose a heterogenous group of membrane-derived particles, including exosomes, microvesicles and apoptotic bodies, which are released into the extracellular environment in response to proinflammatory or proapoptotic stimuli. From earlier studies suggesting that EV shedding constitutes a cellular clearance mechanism, it has become evident that EV formation, secretion and uptake represent important mechanisms of intercellular communication and exchange of a wide variety of molecules, with relevance in both physiological and pathological situations. The putative role of EVs in hemostasis and thrombosis is supported by clinical and experimental studies unraveling how these cell-derived structures affect clot formation (and resolution). From those studies, it has become clear that the prothrombotic effects of EVs are not restricted to the exposure of tissue factor (TF) and phosphatidylserines (PS), but also involve multiplication of procoagulant surfaces, cross-linking of different cellular players at the site of injury and transfer of activation signals to other cell types. Here, we summarize the existing and novel clinical and experimental evidence on the role and function of EVs during arterial and venous thrombus formation and how they may be used as biomarkers as well as therapeutic vectors.
Characteristics of TIMP1, CD63, and β1-Integrin and the Functional Impact of Their Interaction in Cancer
Authors: Justo Beatriz Laís , Jasiulionis Miriam Galvonas
Published: August 2021

Tissue Inhibitor of Metalloproteases 1, also known as TIMP-1, is named for its well-established function of inhibiting the proteolytic activity of matrix metalloproteases. Given this function, many studies were carried out to verify if TIMP-1 was able to interrupt processes such as tumor cell invasion and metastasis. In contrast, many studies have shown that TIMP-1 expression is increased in several types of tumors, and this increase was correlated with a poor prognosis and lower survival in cancer patients. Later, it was shown that TIMP-1 is also able to modulate cell behavior through the induction of signaling pathways involved in cell growth, proliferation, and survival. The mechanisms involved in the regulation of the pleiotropic functions of TIMP-1 are still poorly understood. Thus, this review aimed to present literature data that show its ability to form a membrane complex with CD63 and β1-integrin, and point to N-glycosylation as a potential regulatory mechanism of the functions exerted by TIMP-1. This article reviewed the characteristics and functions performed individually by TIMP1, CD63, and β1-integrin, the roles of the TIMP-1/CD63/β1-integrin complex, both in a physiological context and in cancer, and the regulatory mechanisms involved in its assembly.
Loss of Ift74 Leads to Slow Photoreceptor Degeneration and Ciliogenesis Defects in Zebrafish
Authors: Zhu Panpan , Xu Jingjin , Wang Yadong , Zhao Chengtian
Published: August 2021

Cilia are microtubule-based structures projecting from the cell surface that perform diverse biological functions. Ciliary defects can cause a wide range of genetic disorders known collectively as ciliopathies. Intraflagellar transport (IFT) proteins are essential for the assembly and maintenance of cilia by transporting proteins along the axoneme. Here, we report a lack of Ift74, a core IFT-B protein, leading to ciliogenesis defects in multiple organs during early zebrafish development. Unlike rapid photoreceptor cell death in other ift-b mutants, the photoreceptors of ift74 mutants exhibited a slow degeneration process. Further experiments demonstrated that the connecting cilia of ift74 mutants were initially formed but failed to maintain, which resulted in slow opsin transport efficiency and eventually led to photoreceptor cell death. We also showed that the large amount of maternal ift74 transcripts deposited in zebrafish eggs account for the main reason of slow photoreceptor degeneration in the mutants. Together, our data suggested Ift74 is critical for ciliogenesis and that Ift proteins play variable roles in different types of cilia during early zebrafish development. To our knowledge, this is the first study to show ift-b mutant that displays slow photoreceptor degeneration in zebrafish.
Sample Preparation as a Critical Aspect of Blood Platelet Mitochondrial Respiration Measurements—The Impact of Platelet Activation on Mitochondrial Respiration
Authors: Siewiera Karolina , Labieniec-Watala Magdalena , Wolska Nina , Kassassir Hassan , Watala Cezary
Published: August 2021

Blood platelets are considered as promising candidates as easily-accessible biomarkers of mitochondrial functioning. However, their high sensitivity to various stimulus types may potentially affect mitochondrial respiration and lead to artefactual outcomes. Therefore, it is crucial to identify the factors associated with platelet preparation that may lead to changes in mitochondrial respiration. A combination of flow cytometry and advanced respirometry was used to examine the effect of blood anticoagulants, the media used to suspend isolated platelets, respiration buffers, storage time and ADP stimulation on platelet activation and platelet mitochondria respiration. Our results clearly show that all the mentioned factors can affect platelet mitochondrial respiration. Briefly, (i) the use of EDTA as anticoagulant led to a significant increase in the dissipative component of respiration (LEAK), (ii) the use of plasma for the suspension of isolated platelets with MiR05 as a respiration buffer allows high electron transfer capacity and low platelet activation, and (iii) ADP stimulation increases physiological coupling respiration (ROUTINE). Significant associations were observed between platelet activation markers and mitochondrial respiration at different preparation steps; however, the fact that these relationships were not always apparent suggests that the method of platelet preparation may have a greater impact on mitochondrial respiration than the platelet activation itself.
Hydrothermal-Freeze-Casting of Poly(amidoamine)-Modified Graphene Aerogels towards CO2 Adsorption
Authors: Pruna Alina , Cárcel Alfonso , Benedito Adolfo , Giménez Enrique
Published: August 2021

This article presents novel poly(amidoamine) (PAMAM) dendrimer-modified with partially-reduced graphene oxide (rGO) aerogels, obtained using the combined solvothermal synthesis-freeze-casting approach. The properties of modified aerogels are investigated with varying synthesis conditions, such as dendrimer generation (G), GO:PAMAM wt. ratio, solvothermal temperature, and freeze-casting rate. Scanning electron microscopy, Fourier Transform Infrared spectroscopy, Raman spectroscopy, X-ray photoelectron spectroscopy are employed to characterize the aerogels. The results indicate a strong correlation of the synthesis conditions with N content, N/C ratio, and nitrogen contributions in the modified aerogels. Our results show that the best CO2 adsorption performance was exhibited by the aerogels modified with higher generation (G7) dendrimer at low GO:PAMAM ratio as 2:0.1 mg mL−1 and obtained at higher solvothermal temperature and freeze-casting in liquid nitrogen. The enclosed results are indicative of a viable approach to modify graphene aerogels towards improving the CO2 capture.
Anisotropic Chitosan Scaffolds Generated by Electrostatic Flocking Combined with Alginate Hydrogel Support Chondrogenic Differentiation
Authors: Gossla Elke , Bernhardt Anne , Tonndorf Robert , Aibibu Dilbar , Cherif Chokri , Gelinsky Michael
Published: August 2021

The replacement of damaged or degenerated articular cartilage tissue remains a challenge, as this non-vascularized tissue has a very limited self-healing capacity. Therefore, tissue engineering (TE) of cartilage is a promising treatment option. Although significant progress has been made in recent years, there is still a lack of scaffolds that ensure the formation of functional cartilage tissue while meeting the mechanical requirements for chondrogenic TE. In this article, we report the application of flock technology, a common process in the modern textile industry, to produce flock scaffolds made of chitosan (a biodegradable and biocompatible biopolymer) for chondrogenic TE. By combining an alginate hydrogel with a chitosan flock scaffold (CFS+ALG), a fiber-reinforced hydrogel with anisotropic properties was developed to support chondrogenic differentiation of embedded human chondrocytes. Pure alginate hydrogels (ALG) and pure chitosan flock scaffolds (CFS) were studied as controls. Morphology of primary human chondrocytes analyzed by cLSM and SEM showed a round, chondrogenic phenotype in CFS+ALG and ALG after 21 days of differentiation, whereas chondrocytes on CFS formed spheroids. The compressive strength of CFS+ALG was higher than the compressive strength of ALG and CFS alone. Chondrocytes embedded in CFS+ALG showed gene expression of chondrogenic markers (COL II, COMP, ACAN), the highest collagen II/I ratio, and production of the typical extracellular matrix such as sGAG and collagen II. The combination of alginate hydrogel with chitosan flock scaffolds resulted in a scaffold with anisotropic structure, good mechanical properties, elasticity, and porosity that supported chondrogenic differentiation of inserted human chondrocytes and expression of chondrogenic markers and typical extracellular matrix.