bluuebunny/crossref_metadata_2025 huggingface dataset:
PS-B10-12: A NOVEL PLATFORM FOR A LARGE-VOLUME CSF COLLECTION IN THE FREE-BEHAVING MICE AS A PRECLINICAL MODEL FOR BIOMARKER DEVELOPMENT.
Authors: Nakajima Tsunoe , Takeda Shuko , Ito Yuki , Oyama Akane , Takami Yoichi , Takeya Yasushi , Yamamoto Koichi , Sugimoto Ken , Rakugi Hiromi , Morishita Ryuichi
Published: January 2023

Objectives:

With the emergence of disease-modifying therapies for dementia including Alzheimers disease and vascular dementia, there is an urgent need for the development of better biomarkers detecting early stage of dementia and predicting rate of progression. Cerebrospinal fluid (CSF) provides close representation of pathophysiological changes occurring in the central nervous system, and CSF biomarkers have proven to be useful for the diagnosis and prognosis of dementia. Preclinical work using mouse models would be useful to explore novel CSF biomarkers; however, detailed characterization of CSF has been challenging due to the difficulty in collecting large amount of CSF from mice. Here, we developed a novel technique that allows consistent recovery of CSF in the awake, free-moving mouse.

Methods:

Three-month-old C57/B6 male mice were used in this study. A small incision was made on the dura mater over the cisterna magna and a collecting tube was placed and fixed on the surface of the dura mater that CSF can be drawn via the small hole. Roller pump was connected to the tubing and CSF was continually collected in awake mobile mice.

Results:

We were able to collect a large volume of CSF from an awake mouse over a few days. Brain tissue had no apparent damage after the long-term collection and no detectable level of blood contamination was found in the collected CSF. This technique allowed a serial measurement of CSF biomarkers in a high-temporal resolution and a continuous administration of compounds / drugs into the subarachnoid space of awake mice.

Conclusions:

This technique would provide the opportunity to identify novel CSF biomarkers using mouse models of dementia.
PS-B11-2: MODULATORY EFFECT OF PPAR-GAMMA BY ACETATE ON CARDIORENOMETABOLIC DISTURBANCE ASSOCIATED WITH HIGH FAT DIET-FED INSULIN-RESISTANT MALE WISTAR RATS
Authors: Olaniyi Kehinde Samuel , Amusa Oluwatobi Aminat , Ajadi Isaac Olatoye
Published: January 2023

Objectives:

Insulin resistance (IR) and its comorbidities are critical pathogenic factors to cardiovascular disease (CVD) and chronic kidney disease (CKD). Cardiovascular disease and CKD are the leading cause of death and morbidity worldwide. Short chain fatty acids, particularly acetate has been demonstrated to elicit cardioprotective effects. Herein, we investigated its effects on IR-driven cardiorenal disorder and the possible involvement of peroxisome proliferator-activated receptor-gamma (PPAR-Gamma) in rats exposed to high fat diet (HFD).

Methods:

Ten-week-old male Wistar rats were assigned into three groups (n = 6/group): The groups received distilled water as vehicle (po), 40% HFD with or without 200 mg/kg sodium acetate (po) respectively. The treatments were carried out for 12 weeks.

Results:

HFD caused IR with impaired glucose tolerance and increased cardiac mass without altering the renal mass. Besides, HFD also increased cardiac/renal lipid indices and low-density lipoprotein, malondialdehyde, uric acid and gamma-glutamyl transferase as well as decreased glutathione, nitric oxide concentration and PPAR-Gamma. It also increased plasma urea, creatinine, troponin, and creatinine kinase and increased cardiac but not renal lactate and lactate dehydrogenase. Nevertheless, supplementation with acetate ameliorated theses alterations.

Conclusions:

The present results demonstrate cardiorenometabolic disturbance in HFD-induced IR and accompanied by deficiency in PPAR-Gamma. The results in addition suggest that acetate ameliorates cardiorenometabolic disturbance by increasing PPAR-Gamma and insulin sensitivity with attenuation of oxidative and pro-inflammation.
PS-B12-4: SOLUBLE (PRO)RENIN RECEPTOR LEVEL INCREASED IN A RAT MODEL OF REDUCED UTERINE PERFUSION PRESSURE
Authors: Suda Chikahito , Morimoto Satoshi , Ichihara Atsuhiro
Published: January 2023

Objective:

The (pro)renin receptor [(P)RR)] is a multifunctional protein with roles in angiotensin II-dependent and -independent intracellular cell signaling and as an adaptor protein between the Wnt receptor complex and vacuolar proton-translocating adenosine triphosphatase. The (P)RR is cleaved from the full length (P)RR to generate soluble (P)RR [s(P)RR], reflecting the status of the tissue activity of the (P)RR. We have shown that maternal blood s(P)RR concentrations in early pregnancy are associated with increased blood pressure in late pregnancy, while higher levels of s(P)RR in cord blood in babies are at lower risk of low birth weight. We have also reported that intracellular s(P)RR is increased by hypoxia and interacts with pyruvate dehydrogenase β; subunit (PDHB) to stabilize PDHB protein and pyruvate dehydrogenase (PDH) activity in trophoblast cells, leading to our hypothesis that (P)RR may help maintain oxidative metabolism and efficient energy production during placental ischemia. In this in vivo study, we investigated effects of reduced uterine perfusion pressure (RUPP) on PDH activity and s(P)RR levels in rats.

Design and method:

RUPP was induced by placing sliver clips around the aorta above the iliac bifurcation and the bilateral uterine arcades in pregnant Sprague Dawley rats on gestational day 14 (RUPP rats). Weights of placentas and infants were measured on gestational day 21. mRNA expressions of (P)RR, hypoxia-inducible factor 1-alpha (HIF1a), and PDHB, and protein levels of HIF1a, (P)RR, s(P)RR, PDHB, lactate, and pyruvate, and activity of PDH in placentas were measured on gestational day 21.

Result:

Weight of placentas and infants were significantly decreased in RUPP rats when compared with those of sham rats. mRNA and protein levels of HIF1a were significantly higher in RUPP rats than in control sham rats, indicating that ischemia was actually induced by RUPP. mRNA levels of (P)RR and PDHB were not significantly different between the groups. The protein level of (P)RR was not significantly different between the groups while s(P)RR protein level was significantly higher in RUPP rats than in controls. Concentrations of lactate or pyruvate and PDH activity were not significantly different between the groups.

Conclusion:

PDH activity was not decreased even under the condition of placental ischemia. s(P)RR but not full length (P)RR was increased by placental ischemia. These data suggested that s(P)RR might be increased by placental ischemia to maintain mitochondrial oxidative metabolism in rats.
PS-B12-5: THE INFLUENCE OF CELLULAR AGING ON THE MOLECULES CLEARANCE THAT UNDERGO ENDOCYTOSIS VIA AN ANGIOTENSIN II TYPE 1 RECEPTOR-DEPENDENT PATHWAY.
Authors: Takahashi Toshimasa , Huang Yibin , Yamamoto Koichi , Rakugi Hiromi
Published: January 2023

Objective:

We recently reported that ligand binding to the receptor for advanced glycation end-products (RAGE) or oxidized LDL receptor (LOX-1) activates the G protein-coupled angiotensin II (AII) type 1 receptor (AT1) via a membrane complex of the receptor (2021 Sci Rep, 2021 iScience). The intracellular events triggered by this AT1 activation include signal transduction in a G-protein-dependent pathway and endocytosis of the ligand-receptors complex in a β;-arrestin-dependent pathway. Particularly, little is known about the significance of AT1-induced endocytosis of ligands. In this study, we used amyloid β; (A β;), a known ligand for RAGE, to examine whether cell aging affects the fate of ligands undergoing AT1-dependent endocytosis.

Design and Methods:

Human unvelical vascular endothelial cell (HUVEC) and human brain microvascluar endothelial cell (HBMEC) were continuously cultured to induce replicative senescence. Fluorescent A β; (FA β;) was used to visualize cellular uptake of A β;. Fluorescence/cell number was measured after 24hour application of FA β; (F0) or after 24hour application followed by 24hour washout of FA β; (F1). Clearance of A β; was culculated by (F0-F1)/F0. Cellular aggeragation of A β; was assessed by PROTEOSTAT∨ reagent. Pretreatment with lysosomal inhibitors (Chloroquine, Bafilomycin A), siRNA knockdown of AT1, or trehalose as a lysosomal activator was used if applicable. β;galactosidase assay and real time PCR of p16 mRNA were performed to quantify cellular senescence.

Results:

In HUVECs, pretreatment with Chloroquine or Bafilomycin A markedly decreased A β; clearance. Older HUVEC with passage 12 (P12) and older HBMEC with P20 showed higher β;-galactosidase activity and p16 expression compared with younger cells with P3 and P5, respectively. Clearance of A β; was decreased in older cells compared to younger cells in both cell types. A β; aggregation was higher in older cells than younger cells in both cell types. siRNA of AT1 prominently decreased uptake and aggregation of A β; but did not influence clearance rate of Aβ; in both types of cells. Pretreatment of trehalose significantly restored clearance of Aβ; in older HBMEC.

Conclusions:

These findings indicate that cellular senescence reduces the clearance of molecules that undergo endocytosis via an AT1-dependent pathway. Further investigation will be required, but impaired lysosomal function due to aging might be involved in this mechanism.
PS-B12-9: ASTRAGALOSIDE IV INHIBITS HYDROGEN PEROXIDE-INDUCED APOPTOSIS IN H9C2 CELLS BY ATTENUATING OXIDATIVE STRESS AND MITOCHONDRIAL DAMAGE
Authors: Qi Miaomiao , Wang qiongying , Sun Runmin , Tian Li , Bai Feng , Yu Jing
Published: January 2023

Backgrounds:

Oxidative stress and mitochondrial damage play an important role in the pathology of cardiomyocytes apoptosis. However, the effects of Astragaloside IV(As-IV) on mitochondrial function have not been uncertain. Therefore, we assumed that As-IV inhibits hydrogen peroxide-induced apoptosis in H9c2 cells by attenuating oxidative stress and mitochondrial damage.

Objectives:

To investigate the effects and mechanisms of As-IV on hydrogen peroxide-induced apoptosis in H9c2 cells.

Methods:

H9c2 cells were divided into three groups, including normal control group (cultured without intervention), hydrogen peroxidegroup (cultured with 200 μmol/L hydrogen peroxide for 2 hours), As-IV group (cultured with 100 μmol/L As-IV for 1 hour), As-IV with hydrogen peroxide group (pretreated with 100 μmol/L As-IV for 1 hour, then incubated with hydrogen peroxide for 2 hours). FITC/PI and DCFH-DA are stained to examine levels of apoptosis and ROS receptively. Levels of SOD and MDA were measured. Stained with JC-1 probe to observe the change of mitochondrial membrane potential (MMP). Western-blot was detected to analyze the expression of Drp-1.

Results 1:

The apoptosis rate of hydrogen peroxide group ((13.22 ± 3.17 vs 40.55 ± 13.74)%, P < 0.05) was significantly higher than that of control group, which was lower in As-IV with hydrogen peroxide group((23.43 ± 4.3 vs 40.55 ± 13.74) %, P < 0.05). 2. The ROS levels were higher in hydrogen peroxide group than control group((73.53 ± 5.33 vs 39.02 ± 7.90) %, P < 0.05), which were significant reduced in As-IV with hydrogen peroxide group((57.6 ± 4.63 vs 73.53 ± 5.33) %, P < 0.05) Meanwhile, the activity of MDA was increased and the activity of SOD was decreased in hydrogen peroxide group compared with that in control group((10.97 ± 0.56 vs 4.85 ± 0.55) nmol/mL, P < 0.05), ((1192.23 ± 341.59 vs 2710.95 ± 274.32)U/L, P < 0.05). Compared with hydrogen peroxide group, As-IV with hydrogen peroxide group significantly decrease the activity of MDA and increase the activity of SOD ((6.58 ± 0.79 vs 10.97 ± 0.56) nmol/mL, P < 0.05), ((1984.66 ± 188.81 vs 1192.23 ± 341.59) U/L, P < 0.05). 3. MMP was lower and the expression of Drp1 was higher in hydrogen peroxide group than that in control group ((0.31 ± 0.01 vs 1.52 ± 0.25) %, P < 0.05), ((0.86 ± 0.003 vs 0.45 ± 0.004) %, P < 0.05). Compared with hydrogen peroxide group, MMP was significantly increased and the expression of Drp1 was downregulated ((0.65 ± 0.38 vs 0.31 ± 0.01) %, P < 0.05), ((0.72 ± 0.005 vs 0.86 ± 0.003) %, P < 0.05).

Conclusions:

As-IV exerts protective effects on hydrogen peroxide-induced apoptosis by attenuating oxidative stress and mitochondrial damage.
PS-B12-12: NOVEL PATHOPHYSIOLOGICAL ROLES OF A-SYNUCLEIN IN AGE-RELATED VASCULAR ENDOTHELIAL DYSFUNCTION AND E NHANCED PERMEABILITY.
Authors: Takami Yoichi , Wang Cheng , Yamamoto Koichi , Takeya Yasushi , Nakajima Tuneo , Takeda Shuko , Nakagami Hironori , Kaneda Yasufumi , Rakugi Hiromi
Published: January 2023

Although α-synuclein (SNCA) is a well-known pathological molecule involved in synucleinopathy in neurons, its physiological roles remain largely unknown. We reported that serum SNCA levels have a close inverse correlation with blood pressure and age, which indicates the involvement of SNCA in age-related endothelial dysfunction. Therefore, this study aimed to elucidate the molecular functions of SNCA in the endothelium.

We confirmed that SNCA was expressed in and secreted from endothelial cells (ECs). Exogenous treatment with recombinant SNCA (rSNCA) activated the Akt-eNOS axis and increased nitric oxide (NO) production in ECs. Treatment with rSNCA also suppressed TNF-α and palmitic acid induced NF-κB activation, leading to the suppression of VCAM-1 upregulation and restoration of eNOS downregulation in ECs. As for endogenous SNCA expression, replicative senescence resulted in the attenuation of SNCA expression in cultured ECs, similar to the effects of physiological aging on mice aortas. The siRNA-mediated silencing of SNCA consistently resulted in senescent phenotypes, such as eNOS downregulation, increased β; gal activity, decreased Sirt1 expression, and increased p53 expression, in ECs. Mechanistically, loss-of-function of SNCA upregulated the expression of β;-site amyloid precursor protein cleaving enzyme 1 (BACE1), which was reported to exacerbate eNOS inactivation and permeability in ECs. Addition of SNAP, NO donor, rescued the increment of BACE1, which indicated eNOS downregulation by loss-of-function of SNCA caused BECE1 increment in ECs. Ex vivo assessment of endothelial functions using aortic rings revealed impaired endothelium-dependent acetylcholine-induced relaxation in SNCA knockout (KO) mice. Furthermore, SNCA KO mice, especially those on a high fat diet, displayed elevated blood pressure compared with wild-type (WT) mice; this could be eNOS dysfunction dependent because of the lower difference caused by L NAME administration. In addition, increased depositions of immunoglobulin were found in the aortas of SNCA KO compared with those of WT mice, which indicated enhanced endothelial permeability by in vivo loss-of-function of SNCA. Thus, these results indicate that exogenous and endogenous SNCA in ECs might physiologically maintain vascular integrity, and age-related endothelial dysfunction and enhanced permeability might be partially ascribed to loss-of-function of SNCA in ECs.
PS-C28-3: EFFECTS OF THE OLMESARTAN MEDOXOMIL ON CONTROLLING HYPERTENSION AND REHABILITATION OUTCOMES FOR INPATIENTS IN THE STROKE CARE UNIT
Authors: Matsumoto Shuji , Hoei Takashi , Tojo Ryuji , Nakamura Toshihiro
Published: January 2023

Objective:

Hypertension is the most prevalent risk factor for stroke, based on data from many studies, and has been reported in about 64% of patients with stroke. In patients with severe hypertension, chronic heart failure or history of stroke, the lower limit of cerebral blood flow autoregulation is shifted to a higher blood pressure (BP) than in healthy subjects. Furthermore, in patients with cerebrovascular disease, CBF autoregulation can be disturbed, so that the mean BP necessary to maintain a constant CBF is higher than that in healthy subjects. In the present study, we assessed the effects of the angiotensin II type 1 receptor antagonist olmesartan medoxomil on controlling hypertension and rehabilitation outcomes for hypertensive post-stroke patients in the stroke care unit.

Methods:

Inpatients of both genders, who were aged between 20 and 75 years and had hypertension and a history of a first stroke, were eligible for participation in the present study. In total, 54 patients were enrolled in the study. Research design was an open treatment trial with pre-treatment and post-treatment evaluations for 8 weeks. After screening, the patients were administered olmesartan once daily at a dose of 10 mg for the first 4 weeks of the trial. The dose was increased over the next 4 weeks if a 24-hr SBP/DBP of < 135/80mmHg was not achieved, or if reductions in the 24-hr SBP/DBP of > 10/5 mmHg were not realized. The outcome measures were the 24-h BP (ABPM), heart rate (HR), and rehabilitation outcomes (Brunnstrom stage [BRS], Barthel index [BI] and Mini-Mental State Examination [MMSE] score).

Results:

Olmesartan was well tolerated by all patients, and no complications or adverse events (severe or fatal) were observed during the eight weeks of treatment. The SBP and DBP assessed by ABPM, decreased continuously and significantly after olmesartan administration. In all subjects, the BRS of recovery from hemiplegia in the upper limb, finger, and lower limb showed a significant improvement after 8 weeks of treatment. No decreases in BRS were observed in any of the patients during the study. Also, the BI and MMSE score significantly improved after 8 weeks of treatment.

Conclusions:

Our findings confirmed that olmesartan is an effective antihypertensive drug with good tolerability. Treating hypertensive patients with a history of stroke for eight consecutive weeks with olmesartan decreased 24-hr BP, and improved rehabilitation outcomes. These findings suggest that olmesartan has beneficial effects for the hypertensive post-stroke patients in the stroke care unit.
PS-C28-9: AMBULATORY BP DERIVED SALT SENSITIVITY INDEX AS A PREDICTOR OF THE BP RESPONSE TO RENAL DENERVATION
Authors: Ashton Jonathon Sesa , Carnagarin Revathy , Nolde Janis M , Schlaich Markus
Published: January 2023

Objective:

Renal denervation (RDN) has been consistently shown in recent sham-controlled clinical trials to reduce clinic and ambulatory blood pressure (BP). Salt sensitivity is a critical factor in hypertension pathogenesis and severity, but cumbersome to assess by gold-standard methodology. 24-hour average heart rate and mean arterial pressure dipping, taken by ambulatory blood pressure monitoring (ABPM), can stratify patients into high, moderate and low salt sensitivity index (SSI) risk categories. Here we aimed to assess whether ABPM-derived SSI risk could predict the systolic blood pressure reduction at long-term follow-up in a real-world cohort of RDN patients.

Design and Methods:

60 participants (70.0 ± 10.3 years, 76.3% male) had repeat ABPM assessment as part of a renal denervation long-term follow and compared to their pre-RDN baseline for calculating systolic BP changes. Average time since RDN was 8.7 years ± 1.09 years. Participants were stratified into low, moderate and high SSI Risk groups, respectively. MAP dipping less than 10% and heart rate (HR) greater than 70 indicated high-risk, MAP dipping greater than 10% and HR less than 70 indicated low risk. Remaining participants were classified as moderate risk. Systolic BP changes in risk groups were compared by One-way ANOVA. Multiple regression was computed for systolic BP response, baseline SBP and High SSI Risk status.

Results:

One-way ANOVA indicated a significant treatment effect (p = 0.03) between low, moderate and high salt-sensitivity index risk calculated by ABPM measures with systolic BP reduction of 9.64 ± 3.74 mmHg, 8.41 ± 3.48 mmHg, and 28.20 ± 9.55 mmHg, respectively. Baseline SBP was not significantly different between SSI Risk groups (p = 0.18), but trended upward with increasing SSI. High SSI risk independently correlated with systolic BP reduction (p = 0.02).

Conclusions:

Our preliminary investigation indicates that SSI risk may serve as a simple and easily accessible measure for prediction of the BP response to RDN. However, the influence of pharmacological therapy on these participants is an important extraneous variable requiring testing in prospective or drug-naive RDN cohorts.
PS-C29-2: AZELNIDIPINE IMPROVES INSULIN RESISTANCE IN JAPANESE HYPERTENSIVE PATIENTS WITH METABOLIC SYNDROME
Authors: Ueshiba Hajime
Published: January 2023

Objective:

Metabolic syndrome is related with insulin resistance and the close relation between hypertension and insulin resistance has been demonstrated. On ant-hypertensive drugs, organ-protective action other than antihypertensive action is important and should be considered when we select antihypertensive drugs.

Long-acting calcium antagonists have action of improving insulin resistance other than antihypertensive action. In this study, we examined effect of improving insulin resistance in azelnidipne of long-acting calcium antagonist in Japanese hypertensive patients with metabolic syndrome.

Design and method:

This study included 25 patients (14 men and 11 women; mean age 54.6 years) of Japanese hypertensive patients with metabolic syndrome who had prior antihypertensive treatment with ARB (poor BP control with BP > 140/90 mmHg) or no treatment. These patients were given azelnidipine 16 mg/day and the following indices were measured before and 3–6 months of azelnidipine therapy to compare their variations: systolic BP, diastolic BP, heart rate, fasting plasma glucose, fasting immunoreactive insulin(F-IRI), homeostasis model assessment for insulin resistance(HOMA-R) and HbA1c.

Results:

The mean BP was 153/90 mmHg before azelnidipine therapy but significantly decreased to 137/80 mmHg after therapy. Heart rate also significantly decreased from 88bpm to 81bpm. F-IRI significantly decreased from 15.0 microIU/ml to 10.1 microIU/ml. HOMA-R significantly decreased from 3.66 to 2.40. Other parameters had no significant changes.

Conclusions:

In Japanese hypertensive patients with metabolic syndrome who had prior antihypertensive treatment with ARB (poor BP control) or no treatment, azelnidipine therapy significantly decreased BP and heart rate and improved insulin resistance. It is speculated that this phenomenon is caused by azelnidipine effect of inhibiting the sympathetic nervous system. This study showed that azelnidipine therapy is useful for hypertensive patients with metabolic syndrome.
PS-C30-8: PREGNANT WOMEN WITH STRUGGLING WITH HIGH BLOOD PRESSURE IN PREGNANCY AND ROLE OF NITRIC OXIDE SUPPLEMENTS L-ARGININE FOR PROLONG PERIOD
Authors: Soomro Khalida
Published: January 2023

Nitric oxide is a compound in the body to relax and widen blood vessels Some scientists believe that nitric oxide stimulates the release of certain hormones, such as insulin and human growth hormone. Researchers have performed multiple clinical trials related to nitric oxide supplements that includes L-citrulline and L-arginine and their effectiveness, often with mixed results. Taking nitric oxide supplements offers several heart boosting effects. Reducing arterial stiffness, blood pressure, and improving carotid artery blood flow, by enhancing blood flow in the body they improve performance in sports, promote healing, enhance heart health, and provide many other potential benefits. Preeclampsia, which is a form of high blood pressure that can occur in pregnancy, can be dangerous for both the woman and baby. A 2005 study in the European Journal of Clinical Investigation Trusted Source found that pregnant women who took L-arginine supplements for a prolonged period had lower blood pressure readings than pregnant women who did not take L-arginine. Although researchers need to conduct more studies, the results are promising for women struggling with high blood pressure in pregnancy. However, there are no studies that establish how much nitric oxide supplements people should take to achieve the results.
PS-C30-12: IMPACT OF SARS-COV-2 INFECTION TO RISK AND SEVERITY OF HYPERTENSION IN PREGNANCY AND ITS OUTCOME TO THE NEW BORN
Authors: Pradnyaandara I Gusti Bagus Mulia Agung , Winata I Gde Sastra , Putra I Wayan Artana
Published: January 2023

Objective:

To determine relationship of SARS-CoV-2 infection to the risk and severity of preeclampsia, as well as its impact on newborns.

Design and method:

We performed a systematic search in databases (PubMed, ScienceDirect, ProQuest, and Cochrane Library) for studies examining impact of SARS-CoV-2 infection on pregnancy. Included studies were evaluated for risk of bias based on the Newcastle Ottawa Score. A meta-analysis was conducted using the data extracted from each study. Review Manager (RevMan) 5.4 was utilized to compute the summary of odds ratios (OR), mean differences (MD), and 95% confidence intervals (CI) for the outcomes. Our outcomes of interest are preeclampsia, preeclampsia with severe features, eclampsia, fetal distress and still birth. The other outcomes are preterm birth (< 37 week), instrumental labor, sectio caesaria and birth defect.

Results:

We identified twenty two observational studies involving 1,025,048 pregnancy patients. Based on the analysis, SARS-CoV-2 infection in pregnancy significantly increased the risk of preeclampsia [OR 2.01(95% CI 1.59–2.53; p < 0.00001; I2 = 82%)], and the severity was based on the high prevalence of preeclampsia with severe features [OR 3.04(95% CI 1.19–7.78; p = 0.02; I2 = 91%)] and eclampsia [OR 17.73(95% CI 13.83–22.72;p < 0.00001; I2 = 0%)]. Poor outcome in newborns in terms of incidence of preterm birth [OR 1.65(95% CI 1.54–1.76; p < 0.00001; I2 = 86%)], fetal distress [OR 19.18(95% CI 17.14–21.45; p < 0.00001; I2 = 99%)] and still birth [OR 2.12(95% CI 1.74–2.59; p < 0.00001; I2 = 0%)], were also significantly associated with SARS-CoV-2 infection.

Conclusions:

SARS-CoV-2 infection during pregnancy increases the risk and severity of preeclampsia and gives a poor outcome in newborn.
PS-C33-9: PSYCHOLOGICAL ABNORMALITIES IN PATIENTS WITH RESISTANT HYPERTENSION FOUND NON-ADHERENT BY DIRECTLY OBSERVED TREATMENT INTAKE
Authors: Polonia Jorge J , Barbosa Loide , Silva Jose Alberto , Salgueiro Andre
Published: January 2023

Introduction & aim:

We intend to assess the underlying psychological and behavioural factors in patients with resistant hypertension (HTres) confirmed as non-adherents by directly observed treatment intake (DOTI).

Methods:

We studied with DOTI 68 patients with HTres in outpatient hypertension clinic. 24-h ambulatory BP (ABP) was evaluated before the procedure. In DOTI patients took all medications in the morning for 5 days under the supervision of a technician and performing on the fifth day a second ABP. In some patients a third ABP measurement was repeated 3–6 months after DOTI. All patients responded to appropriate questionnaires evaluating their psychological and behavioural profiles

Results:

Out of the 68 patients 76% were female and 21% diabetics in average ageing 62 years and BMI 30 Kg/m2. Average antihypertensive agents was 4.6 ± 1.2 drugs/day. After DOTI casual, daytime and night-time BP decreased significantly (24 h from 149/82 + 13/13 to 131/74 + 13/9 mm Hg, p < 0.01). Pts (n = 36, i.e. 52.9%) were classified as non-adherents (false HTres) since after DOTI vs previous 24-h ABP there was a significant reduction of mean 24 h BP to < 130/80 mm Hg or a reduction of both 24 h SBP and 24 h DBP by > 10%). Non-adherents i.e false HTres showed significant worse values of disbelief in the potential benefits of treatment, expression of emotions, hostility, anxiety, paranoid ideation, self-blame, physical mobility, smoking habits and social isolation.

Conclusions:

DOTI can be a valuable tool to identify the non-adherence to therapy as a cause of HRes. Non-adherence was associate with worse psychological and behaviour profiles.
PS-C34-4: EVALUATION OF EFFECTS ON BP AND CHOLESTEROL CHANGES AFTER MULTIPLE IMPUTATION FOR MISSING VALUES IN OBSERVATIONAL STUDY DATA OF POLYPILL
Authors: Son Chanseok , Lee Juneyoung
Published: January 2023

Purpose:

The purpose of this study is to prove the effect of polypill on BP and cholesterol reduction effects by analyzing observational study data including missing.

Method:

Medical records were collected for a minimum of 8 weeks and a maximum of 24 weeks after the first prescription of the polypill for each subject for patients who changed their administration to the polypill, which was collected in a retrospective observational study at 71 centers in Korea. In the collected data, there were 1412 subjects, but at 8 weeks, 16 weeks, and 24 weeks, BP and cholesterol information were 30–50% or more missing.

Therefore, the available data were analyzed with GLM and LMM, and complete case data with missing removed and the imputed data by MICE estimated with pmm algorithm at all time points were analyzed by LMM.

Results:

As a result of analyzing the available data with GLM in the improvement effect of SBP, it decreased relatively linearly until the 24th week, and as a result of analyzing the complete case data with LMM, overestimation appeared. However, as a result of analyzing the available data by MICE and LMM, the effect appears until the 8th week, which can be judged as relatively normal data, and is maintained thereafter. Available data can also be analyzed by LMM, and in this case, when the missingness is less than 50%, the results are similar to the case with MICE and LMM analysis. The improvement effects of DBP and LDL-C were similar to SBP.

According to the data of this observational study, the administration of the polypill reduced SBP by 3.7 mmHg, DBP by 2.8 mmHg, and LDL-C by 14.8 mg/dL within 8 weeks of comsuming the polypill compared to the previous treatment. The effect was well maintained by 24 weeks.

Conclusion:

Interpretation of such RWD requires a lot of attention. Basically, errors in analyzing repeatedly measured data with GLM and errors in randomly deleting subjects with missing data should be taken care of. In addition, if there are less than 50% missing, it is convenient to LMM the available data, but if there are more than 50% missing, an unreasonable result has shown.

In conclusion, it is expected that the change of administration to polypill can show additional improvement effects in the control of blood pressure and lipids in patients who are taking two or more of the existing antihypertensive and antidyslipidemic drugs at the same time.
PS-C34-7: EFFECT OF FEBUXOSTAT ON THE LEVEL OF MALONDIALDEHYDE-MODIFIED LOW-DENSITY LIPOPROTEIN, AS AN OXIDANT MARKER, FROM THE SUB-ANALYSES OF PRIZE STUDY
Authors: Teragawa Hiroki , Tanaka Atsushi , Fujii Yuichi , Ueda Tomohiro , Nomura Shuichi , Node Koichi
Published: January 2023

Background:

Febuxostat is a selective xanthine oxidase inhibitor that has been reported to possess antioxidant properties in experimental and animal studies. We previously conducted the program of vascular evaluation under uric acid control by xanthine oxidase inhibitor, febuxostat: multicenter, randomized controlled (PRIZE) study to evaluate the progression of carotid lesions in asymptomatic hyperuricemic patients with carotid atherosclerosis treated with febuxostat for 2 years compared with a control group. In the present sub-analysis study of the PRIZE study, the effect of febuxostat on the level of malondialdehyde modified low density lipoprotein (MDA-LDL), one of the oxidative stress markers, was examined.

Methods:

The subjects were 381 patients enrolled in the PRIZE trial for whom MDA-LDL measurements were available at baseline and at 24 months (182 in the febuxostat group and 199 in the control group). Uric acid (UA) levels, MDA-LDL levels, and MDA-LDL/LDL-C were determined, expressed as the estimated difference from baseline to 24 months, and compared between the two groups. We also examined the relationship between febuxostat dose (10 mg, 20–40 mg, 40–60 mg) and changes in MDA-LDL or MDA-LDL/LDL-C.

Results:

Febuxostat significantly reduced the level of UA, compared with that in the control group, in a dose-dependent manner (p < 0.001). The estimated difference in MDA-LDL/LDL-C from baseline to 24 months was significantly lower in the Febuxostat group than that in the control group (p = 0.025), while the estimated difference in MDA-LDL from baseline to 24 months was not different in the two groups (p = 0.235). There was no significant correlation between the dose of febuxostat and the estimated difference in MDA-L/LDL-C (p = 0.626). Conclusions: In asymptomatic hypereuricemia patients with carotid atherosclerosis, febuxostat may have some antioxidant effect, but the effect may not be dose-dependent.
PS-C35-5: URINARY SODIUM EXCRETION AND THE RISK OF CARDIOVASCULAR DISEASE: A COMMUNITY-BASED COHORT STUDY IN TAIWAN
Authors: Wang Yijie , Chien Kuoliong , Hsu Hsiuching , Lin Hungju , Su Tachen , Chen Mingfong , Lee Yuanteh
Published: January 2023

Urinary sodium excretion is a potential risk factor for cardiovascular diseases (CVD). However, the underlying biological mechanisms and effects of salt sensitivity are unclear. The purpose of this study was to characterize the relative contribution of biological factors to the sodium-CVD association. A total of 2112 participants were enrolled in this study. Structured questionnaires and blood and urine samples were obtained. Twenty-four-hour sodium excretion was estimated using a single overnight urine sample. Hypertension, metabolic syndrome, and overweight status were considered to indicate salt sensitivity. Cox proportional hazard models were used to investigate the effects of salt sensitivity on urinary sodium excretion and CVD risk. The traditional mediation approach was used to calculate the proportion of mediation. The mean age (standard deviation) of the 2112 participants was 54.5 (12.2) years, and they were followed up for a mean of 14.1 [8.1] years. Compared with those in the lowest quartile, the highest baseline urinary sodium excretion (> 4.2 g/24 hours) was associated with a 43% higher CVD risk (hazard ratio, 1.43; 95% confidence interval, 1.02–1.99). Participants with high urinary sodium excretion, hypertension, or metabolic syndrome had a significantly high risk of CVD. The carotid intima-media thickness had the largest mediating effect (accounting for 35% of the sodium-CVD association), followed by systolic blood pressure (33%), left ventricular mass (28%), and diastolic blood pressure (14%). Higher urinary sodium excretion increased the risk of CVD, which was explained largely by carotid media-thickness and systolic blood pressure.
PS-C37-1: EFFICACITY OF COMBINATION ACID ACETYLSALICYLIC AND GLUCOCORTICOID IN TAKAYASU ATERITIS TREATMENT: A CASE CLINIC TAKAYASU ARTERITIS IN VIET NAM
Authors: Nguyen Thao Van
Published: January 2023

A young female 14 years old was detected with Takayasu arteritis in December 2018. We started the therapy with glucocorticoid 20 mg/ day in the first month, and then additive acid acetylsalicylic (Aspirin) 81 mg/day, in addition to the other drugs such as ACEi, a beta-blocker. After 12 months, this therapy bring results positive that were demonstrated via the value C- reactive protein (CRP) from high to normal, and the image of an aortic aneurysm in CT scan is nearly the normal size after treatment. This is the first time that the combination of glucocorticoid and antiplatelet showed benefit in Takayasu disease in Viet Nam. This is a chance for the patients who are diagnosed with Takayasu arteritis, instead of using invasive methods such as aortic stent placement or operations.

JOURNAL/jhype/04.03/00004872-202301001-01260/figure1/v/2023-10-24T163949Z/r/image-jpeg
PS-C37-3: MULTIPLE VISITS TO THE EMERGENCY SERVICE - A CASE OF NUTCRACKER SYNDROME
Authors: Pinto Pedro David Teixeira Marques
Published: January 2023

Background:

Nutcracker Syndrome (NS) is a rare clinical entity consisting of compression of the left renal vein by the superior mesenteric artery as it travels between it and the abdominal aorta. The compressive process leads to different levels of extrinsic renal artery stenosis, from asymptomatic episodes to episodes of macroscopic hematuria, proteinuria, renovascular hypertension, left flank pain, dyspareunia, dysmenorrhea and pelvic varicose veins. It is more prevalent in females and it can be diagnosed at any age, but particularly during the 2nd and 3rd decades of life. Renal veins Doppler ultrasonography is the exam of choice in the initial evaluation, being confirmed by imaging exams such as CT and MRI. The treatment is controversial, ranging from conservative treatment to nephrectomy.

Case Description:

Between 2015 and 2022, a 24-year-old Caucasian woman, without noteworthy personal and family clinical background, resorted several times to the Emergency Department with vomiting, diarrhea and abdominal distension and pain. Numerous alternative diagnoses have been made, such as Acute Gastroenteritis, Urinary Tract Infection, among other.

Due to the large number of emergency episodes, the patient was examined by gastroenterologist who requested an abdominal CT, and diagnosed NS.

The patient underwent an unsuccessful left renal vein stenting, which subsequently forced a renal auto transplantation, which was performed without complications.

Take Home Message:

In the GP daily practice, establishing NS diagnosis is difficult and usually made after the exclusion of other problematics. A great level of suspicion is therefore crucial in patients with diversified clinical findings.
PS-C37-6: IDENTIFICATION OF ALDOSTERONE-DRIVER SOMATIC MUTATIONS IN CELL-FREE DNA FROM ADRENAL VEIN SAMPLES OF PRIMARY ALDOSTERONISM PATIENTS
Authors: Nazri Siti Khadijah Syed Mohammed , Zakaria Rozman , Murad Nor Azian Abdul , Muhammad Rohaizak , Tan Geok Chin , Sukor Norlela , Azizan Elena Aisha
Published: January 2023

Objective:

Cell-free DNA fragments (cf-DNA) of tumour cells are often found in the blood downstream to the tumour due to the high apoptosis or necrosis rate of the cells. Primary aldosteronism (PA), a curable cause of secondary hypertension, are commonly due to an autonomous aldosterone-producing adenoma that harbours a somatic mutation in an aldosterone-driver gene. We thus aimed to see if genotyping of cf-DNA for mutations in aldosterone-driver genes could be used as a biomarker for APA.

Design and Methods:

Genotyping of cf-DNA from the adrenal vein samples (AVS) of PA patients was performed using the Agena MassARRAY platform and validated by Sanger sequencing. In this study, six samples of cf-DNA from the left and right adrenals of three PA patients were interrogated.

Results:

Of the three PA patients, two had left adrenalectomy and one had right adrenalectomy. CTNNB1 S45 mutation was detected by the Agena MassArray platform in the cf-DNA of both right adrenal and left adrenal vein samples of all patients (Patient 1, Patient 2 and Patient 3). In Patient 1, Sanger sequencing confirmed a CTNNB1 S45del mutation in both cf-DNA of right adrenal and left adrenal vein samples. In Patient 3, who was found to have left adrenal hyperplasia, the cf-DNA of the right adrenal and left adrenal vein samples was also detected to have a KCNJ5 G151R mutation by the Agena MassArray platform which was confirmed by Sanger sequencing. Interestingly, only the cf-DNA of the left adrenal was found to have a CACNA1D S672L mutation in Patient 3.

Conclusion:

These results suggest that genotyping cf-DNA from AVS samples are able to detect aldosterone-driver mutations present in the APA. However, as AVS is an invasive procedure, it would be best if genotyping of cf-DNA from periphery blood can be performed. Therefore, further work is needed to ensure this strategy can be non-invasive as then it can be used as a screening method before AVS.
PS-P11-1: ASSESSING THE KNOWLEDGE, ATTITUDE AND AWARENESS OF URBAN HYPERTENSIVE PATIENTS FOR METABOLIC SYNDROME
Authors: Patil Vishal , Patil Mansi
Published: January 2023

Objectives:

The aim of this study was to assess the awareness, knowledge, and attitude of hypertensive patients for metabolic syndrome and its relation to demographic data in those attending the out-patient services.

Design and Methods:

400 known hypertensive patients between the age 18–60 years were recruited. Data was collected using a 2-parts closed ended questionnaire using a 4-point Likert scale. Data was analysed using both descriptive and inferential statistics (student's t-test, Pearson correlation coefficient and Spearman correlation coefficient).

Results:

A significant relationship was established between knowledge score and age of the participants (p = 0.002), but not between age and awareness and attitude (p > ; 0.05). A significant relation was observed between the duration of metabolic syndrome and knowledge score (p < 0.05). There is a significant relationship between knowledge score and educational level (p < 0.05). There is a significant difference between men's and women's awareness scores (p < 0.005), but this difference is not seen in other aspects (knowledge, attitude) (p > ; 0.05). Women's awareness score was more than men's (p = 0.0007). Data analysis showed that there is a significant relationship between awareness and knowledge (P = 0.003), and awareness and attitude (P = 0.0001).

Conclusions:

The participants’ blood pressure was not controlled in spite of good awareness and attitude. The knowledge score was low and community-based programs to improve knowledge should be initiated. Programs like May Measurement Month, regular BP screenings, and awareness around medication compliance should be encouraged in the community.
PS-P11-2: THE PATTERN OF PHYSICAL DISABILITY AND DETERMINANTS OF ACTIVITIES OF DAILY LIVING AMONG DIABETIC PATIENTS IN BANGLADESH: A CROSS-SECTIONAL ANALYSIS.
Authors: Ahmed Sabrina , Faruque Mithila , Moniruzzaman Mohammad , Roby Naym Uddin , Ashraf Fatema , Miura Katsuyuki , Ahmed MSA Mansur
Published: January 2023

Objective:

Diabetes mellitus is a known predictor of physical disability and decline in activities of daily living (ADL); however, there are existing controversies about the factors explaining the association between diabetes and disability. Therefore, we assessed the proportion of physical disabilities and possible factors associated with ADL decline among diabetic patients in Bangladesh.

Design and Methods:

We conducted a cross-sectional study among 480 diabetic individuals from a tertiary level hospital in Dhaka, Bangladesh, aged between 40 and 85 years. For determining the ADL decline, we used the Katz Index Scoring, the highest score of 6 indicates no ADL decline. We divided patients into two groups depending on ADL impairment to compare the population characteristics using the unpaired Students t-test or Wilcoxon rank-sum test for continuous variables and the chi-squared test for categorical variables. Age, sex, educational attainment, household expenditure, BMI, HbA1c, hypertension, and medication adherence to anti-diabetic drugs were included in the statistical models. We defined any ADL decline as an event, and multivariable logistic regression was performed to assess the factors associated with ADL decline.

Results:

The mean age of the participants was 59.0 (standard deviation [SD], 7.0) years. The majority of the participants (76.3%) had at least some sort of physical disability. Age, educational attainment, household expenditure, systolic blood pressure, waist circumference, quality of life, and comorbidities were different among the groups stratified by ADL impairment status. In multivariable logistic regression analysis after adjusting for all covariates simultaneously, age (OR, 95% CI: 1.35, 1.20–1.75), lower educational attainment (0.97, 0.94–0.99), BMI (1.40, 1.12–1.75), existing comorbidities (2.79, 1.48–5.25) and uncontrolled diabetes (1.35,1.10–1.45) were independently associated with ADL decline among diabetic population.

Conclusions:

Physical disability was common, and ADL decline was associated with age, educational attainment, BMI, comorbidities, and uncontrolled diabetes among diabetic patients in Bangladesh.
PS-P12-11: INFECTION CONTROL PRACTICES OF HEALTHCARE WORKERS INFECTED WITH COVID-19 IN TONDO MEDICAL CENTER FROM MARCH 2020 TO MAY 2021
Authors: Gracilla Sheryl Joy Esteban , Quebral Joseph
Published: January 2023

Background:

COVID-19 has put the lives of our healthcare workers at the forefront of the battle against pandemic. Understanding how this pandemic directly affects them is vital in ensuring safe and continuous provision of service to the public. Reviewing their infection control practices is imperative in protecting their health and the patients they serve. This study aimed to determine the infection control practices of healthcare workers infected with COVID-19 in Tondo Medical Center from March 2020 to May 2021.

Methodology:

This is descriptive retrospective study involving healthcare workers of Tondo Medical Center infected with COVID-19 from March 2020 to May 2021. Demographic data, exposure history, clinical manifestations and infection control practices data were surveyed, encoded and tabulated. Categorical variables were presented as frequencies and percentages.

Results:

A total of 425 healthcare workers developed COVID-19 from March 2020 to April 2021. Their mean age was 36.4 years; more female (57.4%) and single (56.0%) staff were affected. Most cases (41.4%) came from or low/lowest risk areas. Three clustering of cases were identified. More than half of the respondents (54.4%) had unknown exposure; housemates/relatives (21.0%) were the most common source of known exposure. Three out of 10 respondents were asymptomatic; majority had mild symptoms (62.2%), the most common of which was the loss of taste/smell (26.5%). Adherence to wearing of mask was highest in the community (99.5%) than in the workplace (93.4%) and least at home (78.3%). Majority practiced social distancing in the community (97.9%) and in the workplace (95.5%). Almost half (58.7%) practiced 1-meter social distancing. Respondents practiced frequent handwashing both at home (92.2%) and in the workplace (91.9%). Only 1–2 out of 10 washed their hands for 40 seconds or more. Majority employed hand hygiene with alcohol-based rub for less than 20 seconds in the workplace (71.6%) and at home (69.5%). Majority (98.3%) performed hand hygiene after coughing or sneezing and used tissue (44.4%). Most of the respondents decontaminated their work area before and after work (66.6%). Alcohol was most used in the workplace (70.7%). More than half (59.6%) ate in the pantry, mostly alone (65.5%). Most cleaned their cellphones daily (88.4%). Majority brought alcohol with them (97.2%) and almost half (47.5%) wore a mask for 4–8 hours.

Conclusion:

Some gaps have been identified in the knowledge and practices of infection control of healthcare workers infected with COVID-19 at Tondo Medical Center; hence reinforcement programs including refresher trainings and policy enhancement are warranted.