bluuebunny/crossref_metadata_2025 huggingface dataset:
mcr-1-Mediated Colistin Resistance and Genomic Characterization of Antimicrobial Resistance in ESBL-Producing Salmonella Infantis Strains from a Broiler Meat Production Chain in Italy
Authors: Casagrande Proietti Patrizia , Musa Laura , Stefanetti Valentina , Orsini Massimiliano , Toppi Valeria , Branciari Raffaella , Blasi Francesca , Magistrali Chiara Francesca , Capomaccio Stefano , Kika Tana Shtylla , Franciosini Maria Pia
Published: May 2022

This work aimed to evaluate phenotypically and genotypically the colistin susceptibility of 85 Salmonella Infantis strains isolated in Italy from the broiler production chain, and to apply a whole-genome approach for the determination of genes conferring antimicrobial resistance (AMR). All isolates were tested by the broth microdilution method to evaluate the colistin minimum inhibitory concentrations (MICs). A multiplex PCR was performed in all isolates for the screening of mcr-1, mcr-2, mcr-3 mcr-4, mcr-5 genes and whole-genome sequencing (WGS) of six S. Infantis was applied. Three out of 85 (3.5%) S. Infantis strains were colistin resistant (MIC values ranged from 4 to 8 mg/L) and mcr-1 positive. The mcr-1.1 and mcr-1.2 variants located on the IncX4 plasmid were detected in three different colistin-resistant isolates. The two allelic variants showed identical sequences. All six isolates harbored blaCTXM-1, aac(6′)-Iaa and gyrA/parC genes, mediating, respectively, beta-lactam, aminoglycoside and quinolone resistance. The pESI-megaplasmid carrying tet(A) (tetracycline resistance), dfrA1, (trimethoprim resistance) sul1, (sulfonamide resistance) and qacE (quaternary ammonium resistance) genes was found in all isolates. To our knowledge, this is the first report of the mcr-1.2 variant described in S. Infantis isolated from broilers chickens. Our results also showed a low prevalence of colistin- resistance, probably due to a reduction in colistin use in poultry. This might suggest an optimization of biosecurity control both on farms and in slaughterhouses.
Antimicrobial Nanomaterials for Food Packaging
Authors: Suvarna Vasanti , Nair Arya , Mallya Rashmi , Khan Tabassum , Omri Abdelwahab
Published: May 2022

Food packaging plays a key role in offering safe and quality food products to consumers by providing protection and extending shelf life. Food packaging is a multifaceted field based on food science and engineering, microbiology, and chemistry, all of which have contributed significantly to maintaining physicochemical attributes such as color, flavor, moisture content, and texture of foods and their raw materials, in addition to ensuring freedom from oxidation and microbial deterioration. Antimicrobial food packaging systems, in addition to their function as conventional food packaging, are designed to arrest microbial growth on food surfaces, thereby enhancing food stability and quality. Nanomaterials with unique physiochemical and antibacterial properties are widely explored in food packaging as preservatives and antimicrobials, to extend the shelf life of packed food products. Various nanomaterials that are used in food packaging include nanocomposites composing nanoparticles such as silver, copper, gold, titanium dioxide, magnesium oxide, zinc oxide, mesoporous silica and graphene-based inorganic nanoparticles; gelatin; alginate; cellulose; chitosan-based polymeric nanoparticles; lipid nanoparticles; nanoemulsion; nanoliposomes; nanosponges; and nanofibers. Antimicrobial nanomaterial-based packaging systems are fabricated to exhibit greater efficiency against microbial contaminants. Recently, smart food packaging systems indicating the presence of spoilage and pathogenic microorganisms have been investigated by various research groups. The present review summarizes recent updates on various nanomaterials used in the field of food packaging technology, with potential applications as antimicrobial, antioxidant equipped with technology conferring smart functions and mechanisms in food packaging.
Combination of C-Reactive Protein and Procalcitonin in Distinguishing Fungal from Bacterial Infections Early in Immunocompromised Children
Authors: Liu Yingli , Zhang Xiaoli , Yue Tianfang , Tang Yanlai , Ke Zhiyong , Li Yu , Luo Xuequn , Huang Libin
Published: May 2022

Invasive fungal infection (IFI) is life-threatening in children with cancer and hematology disorders, especially when diagnosis and treatment are delayed. Conventional β-D-glucan and galactomannan tests have poor positive predictive values in the diagnosis of IFI in children with cancer. This study aims to access the diagnostic performance of C-reactive protein (CRP) and procalcitonin (PCT) in differentiating IFI from bacterial bloodstream infections in children with malignant and hematology disorders. CRP and PCT levels were measured in samples taken from patients between 12 and 24 h after fever onset, of which 24 and 102 were in the IFI and bacterial groups, respectively. We found that the CRP levels were much higher in the IFI group than the bacterial group (100.57 versus 40.04 mg/L, median, p < 0.001), while the PCT levels remained significantly lower (0.45 versus 1.29 μg/L, median, p = 0.007). Both CRP and PCT showed significant diagnostic utilities with an area under the curve (AUC) of 0.780 (95% CI, 0.664–0.896, p < 0.001) and 0.731 (95% CI, 0.634–0.828, p < 0.001) when using the cut-off values of 94.93 mg/L and 2.00 μg/L, respectively. However, the combined biomarker of CRP and PCT yielded a better diagnostic performance with an AUC of 0.934 (95% confidential interval (CI), 0.881–0.987, p < 0.001), which was significantly higher than that of CRP or PCT (both p < 0.001), with a sensitivity of 87.5% and a specificity of 87.3%. Our study demonstrates high levels of CRP combined with low PCT could differentiate IFI from bacterial bloodstream infections in immunocompromised children.
Skin Care Product Rich in Antioxidants and Anti-Inflammatory Natural Compounds Reduces Itching and Inflammation in the Skin of Atopic Dermatitis Patients
Authors: Zhang Yu , Heinemann Nina , Rademacher Franziska , Darvin Maxim E. , Raab Christian , Keck Cornelia M. , Vollert Henning , Fluhr Joachim W. , Gläser Regine , Harder Jürgen , Meinke Martina C.
Published: May 2022

The atopic dermatitis (AD) complex pathogenesis mechanism reveals marked changes of certain signaling factors as well as some morphological alterations in the epidermis. Reduced resilience against environmental factors and oxidative stress often makes the treatment with corticosteroids or tacrolismus ointments indispensable. In view of the correlation between oxidative stress and AD pathological factors, antioxidants can be incorporated into AD management strategies. This study investigates a curly kale, apple and green tea-containing natural extract rich in antioxidants for its effects on signaling inflammatory molecules and skin barrier enhancement in human epidermal keratinocytes- (NHEKs) based cell assays. Furthermore, the skin penetration on porcine ears was measured ex vivo using Raman micro spectroscopy. Finally, in a double-blind half-side, placebo-controlled clinical study, the effects of a formulation containing this extract were analyzed for the influence of lesion severity, epidermal barrier function, and pruritus in mild to moderately AD patients. Summarizing our results: The extract reduces expression of inflammatory cytokines in keratinocytes and increases barrier-related molecules. The verum formulation with a very high antioxidant capacity used in AD patients with mild to moderate lesions reduces itching, local SCORAD, and improves barrier function and the hydration of skin lesions.
Investigation of DHA-Induced Regulation of Redox Homeostasis in Retinal Pigment Epithelium Cells through the Combination of Metabolic Imaging and Molecular Biology
Authors: Bianchetti Giada , Clementi Maria Elisabetta , Sampaolese Beatrice , Serantoni Cassandra , Abeltino Alessio , De Spirito Marco , Sasson Shlomo , Maulucci Giuseppe
Published: May 2022

Diabetes-induced oxidative stress leads to the onset of vascular complications, which are major causes of disability and death in diabetic patients. Among these, diabetic retinopathy (DR) often arises from functional alterations of the blood-retinal barrier (BRB) due to damaging oxidative stress reactions in lipids, proteins, and DNA. This study aimed to investigate the impact of the ω3-polyunsaturated docosahexaenoic acid (DHA) on the regulation of redox homeostasis in the human retinal pigment epithelial (RPE) cell line (ARPE-19) under hyperglycemic-like conditions. The present results show that the treatment with DHA under high-glucose conditions activated erythroid 2-related factor Nrf2, which orchestrates the activation of cellular antioxidant pathways and ultimately inhibits apoptosis. This process was accompanied by a marked increase in the expression of NADH (Nicotinamide Adenine Dinucleotide plus Hydrogen) Quinone Oxidoreductase 1 (Nqo1), which is correlated with a contextual modulation and intracellular re-organization of the NAD+/NADH redox balance. This investigation of the mechanisms underlying the impairment induced by high levels of glucose on redox homeostasis of the BRB and the subsequent recovery provided by DHA provides both a powerful indicator for the detection of RPE cell impairment as well as a potential metabolic therapeutic target for the early intervention in its treatment.
The Antioxidant Activity of Thymus serpyllum Extract Protects against the Inflammatory State and Modulates Gut Dysbiosis in Diet-Induced Obesity in Mice
Authors: Ruiz-Malagón Antonio Jesús , Rodríguez-Sojo María Jesús , Hidalgo-García Laura , Molina-Tijeras José Alberto , García Federico , Pischel Ivo , Romero Miguel , Duarte Juan , Diez-Echave Patricia , Rodríguez-Cabezas María Elena , Rodríguez-Nogales Alba , Gálvez Julio
Published: May 2022

Nowadays, there is an increasing interest in alternative therapies in the treatment of metabolic syndrome that combine efficacy and safety profiles. Therefore, this study aimed to evaluate the effect of an extract of Thymus serpyllum, containing rosmarinic acid, on high-fat diet (HFD)-induced obesity mice, highlighting the impact of its antioxidant activity on the inflammatory status and gut dysbiosis. The extract was administered daily (50, 100 and 150 mg/kg) in HFD-fed mice. The treatment reduced body weight gain, glucose and lipid metabolic profiles. Moreover, the extract ameliorated the inflammatory status, with the c-Jun N-terminal kinases (JUNK) pathway being involved, and showed a significant antioxidant effect by the reduction of radical scavenging activity and the mitigation of lipid peroxidation. Moreover, the extract was able to modulate the altered gut microbiota, restoring microbial richness and diversity, and augmenting the counts of short-chain fatty acid producing bacteria, which have been associated with the maintenance of gut permeability and weight regulation. In conclusion, the antioxidant activity of Thymus serpyllum extract displayed a positive impact on obesity and its metabolic alterations, also reducing systemic inflammation. These effects may be mediated by modulation of the gut microbiota.
An In Vitro Study of Saffron Carotenoids: The Effect of Crocin Extracts and Dimethylcrocetin on Cancer Cell Lines
Authors: Hatziagapiou Kyriaki , Nikola Olti , Marka Sofia , Koniari Eleni , Kakouri Eleni , Zografaki Maria-Eleftheria , Mavrikou Sophie S. , Kanakis Charalabos , Flemetakis Emmanouil , Chrousos George P. , Kintzios Spyridon , Lambrou George I. , Kanaka-Gantenbein Christina , Tarantilis Petros A.
Published: May 2022

Crocus sativus L. has various pharmacological properties, known for over 3600 years. These properties are attributed mainly to biologically active substances, which belong to the terpenoid group and include crocins, picrocrocin and safranal. The aim of the current work was to examine the effects of crocins (CRCs) and their methyl ester derivate dimethylcrocetin (DMCRT) on glioblastoma and rhabdomyosarcoma cell lines, in terms of cytotoxicity and gene expression, implicated in proapoptotic and cell survival pathways. Cell cytotoxicity was assessed with Alamar Blue fluorescence assay after treatment with saffron carotenoids for 24, 48 and 72 h and concentrations ranging from 22.85 to 0.18 mg/mL for CRCs and 11.43 to 0.09 mg/mL for DMCRT. In addition, BAX, BID, BCL2, MYCN, SOD1, and GSTM1 gene expression was studied by qRT-PCR analysis. Both compounds demonstrated cytotoxic effects against glioblastoma and rhabdomyosarcoma cell lines, in a dose- and time-dependent manner. They induced apoptosis, via BAX and BID upregulation, MYCN and BCL-2, SOD1, GSTM1 downregulation. The current research denotes the possible anticancer properties of saffron carotenoids, which are considered safe phytochemicals, already tested in clinical trials for their health promoting properties.
Dipeptide of ψ-GSH Inhibits Oxidative Stress and Neuroinflammation in an Alzheimer’s Disease Mouse Model
Authors: Raza Abbas , Xie Wei , Kim Kwan-Hyun , Dronamraju Venkateshwara Rao , Williams Jessica , Vince Robert , More Swati S.
Published: May 2022

Supplementation of glutathione (GSH) levels through varying formulations or precursors has thus far appeared to be a tenable strategy to ameliorate disease-associated oxidative stress. Metabolic liability of GSH and its precursors, i.e., hydrolysis by the ubiquitous γ-glutamyl transpeptidase (γ-GT), has limited successful clinical translation due to poor bioavailability. We addressed this problem through the design of γ-GT-resistant GSH analogue, ψ-GSH, which successfully substituted in GSH-dependent enzymatic systems and also offered promise as a therapeutic for Alzheimer’s disease (AD). With the aim to improve its bioavailability, we studied the utility of a ψ-GSH precursor, dipeptide 2, as a potential AD therapeutic. Compound 2 retains the γ-GT stable ureide linkage and the thiol group for antioxidant property. By engaging glutathione synthetase, compound 2 was able to generate ψ-GSH in vivo. It was found to be a modest cofactor of glutathione peroxidase and prevented cytotoxicity of Aβ1–42-aggregates in vitro. Studies of compound 2 in an acute AD model generated by intracerebroventricular injection of Aβ1–42 showed cognitive benefits, which were augmented by its combination with glycine along with mitigation of oxidative stress and inflammatory pathology. Collectively, these results support further optimization and evaluation of ψ-GSH dipeptide as a potential therapeutic in transgenic AD models.
Antioxidant and Anti-Inflammatory Effect of the Consumption of Powdered Concentrate of Sechium edule var. nigrum spinosum in Mexican Older Adults with Metabolic Syndrome
Authors: Arista-Ugalde Taide Laurita , Santiago-Osorio Edelmiro , Monroy-García Alberto , Rosado-Pérez Juana , Aguiñiga-Sánchez Itzen , Cadena-Iñiguez Jorge , Gavia-García Graciela , Mendoza-Núñez Víctor Manuel
Published: May 2022

Metabolic syndrome (MetS) has a high prevalence in older adults and is a risk factor for cardiovascular diseases and complications of old age. It has also been related to oxidative stress (OxS) and chronic inflammation (CI) and their consequent alterations. Therefore, it is important to propose therapeutic alternatives such as the consumption of Sechium edule (Chayote), since hypoglycemic, hypotensive, and lipogenesis inhibitor properties are attributed to it. We carried out a study in 81 older adults (OA) with MetS to determine the effect of consumption of chayote powder concentrate (500 mg, three times a day) for six months, with a baseline measurement, at three and six months in an experimental group (EG) (n = 41) and a placebo group (PG) (n = 40), all with a diagnosis of MetS according to the criteria of National Adult Treatment Panel of the National Cholesterol Program III (NCEP/ATP III). Anthropometric, biochemical, OxS markers, and inflammation measurements were performed on all participants, basal, three, and six months after. A statistically significant decrease was found in the concentration of lipoperoxides (TBARS), 8-isoprostanes, 8-OHdG, oxidative stress score (OSS), HbA1c, blood pressure, and in the number of MetS diagnostic criteria, as well as an increase in total antioxidant status (TAS), antioxidant gap (GAP), superoxide dismutase (SOD), interleukin 10 (IL-10), and HDL-cholesterol in EG. The results suggest that the consumption of Sechium edule powder has a hypotensive, hypoglycemic, antioxidant, and anti-inflammatory effect in OA with MetS and reduced the percentage of patients with MetS.
Over-Expression of Dehydroascorbate Reductase Improves Salt Tolerance, Environmental Adaptability and Productivity in Oryza sativa
Authors: Kim Young-Saeng , Park Seong-Im , Kim Jin-Ju , Shin Sun-Young , Kwak Sang-Soo , Lee Choon-Hwan , Park Hyang-Mi , Kim Yul-Ho , Kim Il-Sup , Yoon Ho-Sung
Published: May 2022

Abiotic stress induces reactive oxygen species (ROS) generation in plants, and high ROS levels can cause partial or severe oxidative damage to cellular components that regulate the redox status. Here, we developed salt-tolerant transgenic rice plants that overexpressed the dehydroascorbate reductase gene (OsDHAR1) under the control of a stress-inducible sweet potato promoter (SWPA2). OsDHAR1-expressing transgenic plants exhibited improved environmental adaptability compared to wild-type plants, owing to enhanced ascorbate levels, redox homeostasis, photosynthetic ability, and membrane stability through cross-activation of ascorbate-glutathione cycle enzymes under paddy-field conditions, which enhanced various agronomic traits, including root development, panicle number, spikelet number per panicle, and total grain yield. dhar2-knockdown plants were susceptible to salt stress, and owing to poor seed maturation, exhibited reduced biomass (root growth) and grain yield under paddy field conditions. Microarray revealed that transgenic plants highly expressed genes associated with cell growth, plant growth, leaf senescence, root development, ROS and heavy metal detoxification systems, lipid metabolism, isoflavone and ascorbate recycling, and photosynthesis. We identified the genetic source of functional genomics-based molecular breeding in crop plants and provided new insights into the physiological processes underlying environmental adaptability, which will enable improvement of stress tolerance and crop species productivity in response to climate change.
Altered Mitochondrial Opa1-Related Fusion in Mouse Promotes Endothelial Cell Dysfunction and Atherosclerosis
Authors: Chehaitly Ahmad , Guihot Anne-Laure , Proux Coralyne , Grimaud Linda , Aurrière Jade , Legouriellec Benoit , Rivron Jordan , Vessieres Emilie , Tétaud Clément , Zorzano Antonio , Procaccio Vincent , Joubaud Françoise , Reynier Pascal , Lenaers Guy , Loufrani Laurent , Henrion Daniel
Published: May 2022

Flow (shear stress)-mediated dilation (FMD) of resistance arteries is a rapid endothelial response involved in tissue perfusion. FMD is reduced early in cardiovascular diseases, generating a major risk factor for atherosclerosis. As alteration of mitochondrial fusion reduces endothelial cells’ (ECs) sprouting and angiogenesis, we investigated its role in ECs responses to flow. Opa1 silencing reduced ECs (HUVECs) migration and flow-mediated elongation. In isolated perfused resistance arteries, FMD was reduced in Opa1+/− mice, a model of the human disease due to Opa1 haplo-insufficiency, and in mice with an EC specific Opa1 knock-out (EC-Opa1). Reducing mitochondrial oxidative stress restored FMD in EC-Opa1 mice. In isolated perfused kidneys from EC-Opa1 mice, flow induced a greater pressure, less ATP, and more H2O2 production, compared to control mice. Opa1 expression and mitochondrial length were reduced in ECs submitted in vitro to disturbed flow and in vivo in the atheroprone zone of the mouse aortic cross. Aortic lipid deposition was greater in Ldlr−/--Opa1+/- and in Ldlr−/--EC-Opa1 mice than in control mice fed with a high-fat diet. In conclusion, we found that reduction in mitochondrial fusion in mouse ECs altered the dilator response to shear stress due to excessive superoxide production and induced greater atherosclerosis development.
Peter Eckl: Research on the Pro-/Antioxidant Balance
Authors: Bresgen Nikolaus , Siems Werner
Published: May 2022

Peter Maria ECKL started his scientific career in the late 1970s at the Paris-Lodron University of Salzburg working in the field of radiation research [...]
Antioxidants, Oxidative Stress, and Non-Communicable Diseases
Authors: Crisóstomo Luís , Oliveira Pedro F. , Alves Marco G.
Published: May 2022

Non-communicable diseases have become the leading cause of death, morbidity, and loss of healthy years worldwide, according to the World Health Organization [...]
The Pharma-Nutritional Role of Antioxidant Phytochemicals in Health and Disease
Authors: Davinelli Sergio , Scapagnini Giovanni
Published: May 2022

There is an increasing number of disease areas where nutritional and pharmacological applications complement each other [...]
Alleviation of Oral Exposure to Aflatoxin B1-Induced Renal Dysfunction, Oxidative Stress, and Cell Apoptosis in Mice Kidney by Curcumin
Authors: Wang Yingjie , Liu Fangju , Zhou Xin , Liu Mengru , Zang Haoran , Liu Xiao , Shan Anshan , Feng Xingjun
Published: May 2022

Aflatoxin B1 is a contaminant widely found in food and livestock feed, posing a major threat to human and animal health. Recently, much attention from the pharmaceutical and food industries has been focused on curcumin due to its strong antioxidant capacity. However, the therapeutic impacts and potential mechanisms of curcumin on kidney damage caused by AFB1 are still incomplete. In this study, AFB1 triggered renal injury in mice, as reflected by pathological changes and renal dysfunction. AFB1 induced renal oxidative stress and interfered with the Keap1–Nrf2 pathway and its downstream genes (CAT, SOD1, NQO1, GSS, GCLC, and GCLM), as manifested by elevated oxidative stress metabolites and reduced antioxidant enzymes activities. Additionally, AFB1 was found to increase apoptotic cells percentage in the kidney via the TUNEL assay, along with increased expression of Cyt-c, Bax, cleaved-Caspase-3, Caspase-9, and decreased expression of Bcl-2 at the transcriptional and protein levels; in contrast, for mice given curcumin, there was a significant reversal in kidney coefficient, biochemical parameters, pathological changes, and the expression of genes and proteins involved in oxidative stress and apoptosis. These results indicate that curcumin could antagonize oxidative stress and apoptosis to attenuate AFB1-induced kidney damage.
Reactive Human Plasma Glutathione Peroxidase Mutant with Diselenide Bond Succeeds in Tetramer Formation
Authors: Fan Zhenlin , Yan Qi , Song Jian , Wei Jingyan
Published: May 2022

Plasma glutathione peroxidase (GPx3) belongs to the GPx superfamily, and it is the only known secreted selenocysteine (Sec)−containing GPx in humans. It exists as a glycosylated homotetramer and catalyzes the reduction of hydrogen peroxide and lipid peroxides, depending on the Sec in its active center. In this study, a previously reported chimeric tRNAUTuT6 was used for the incorporation of Sec at the UAG amber codon, and the mature form of human GPx3 (hGPx3) without the signal peptide was expressed in amber−less E. coli C321.ΔA.exp. Reactive Sec−hGPx3, able to reduce H2O2 and tert−butyl hydroperoxide (t−BuOOH), was produced with high purity and yield. Study of the quaternary structure suggested that the recombinant Sec−hGPx3 contained an intra−molecular disulfide bridge but failed to form tetramer. Mutational and structural analysis of the mutants with three Cys residues, individually or jointly replaced with Ser, indicated that the formation of intra−molecular disulfide bridges involved structure conformational changes. The secondary structure containing Cys77 and Cys132 was flexible and could form a disulfide bond, or form a sulfhydryl–selenyl bond with Sec49 in relative mutants. Mutation of Cys8 and Cys132 to Sec8 and Sec132 could fix the oligomerization loop through the formation of diselenide bond, which, in turn, facilitated tetramer formation and noticeably improved the GPx activity. This research provides an important foundation for the further catalysis and functional study of hGPx3.
Towards the Implementation Process of Accessible Virtual Campuses in Higher Education Institutions in Latin America
Authors: Vásquez Francisco Sánchez , Pérez-Arriaga Juan Carlos , Vega Gerardo Contreras , Luján-Mora Sergio , Tortosa Salvador Otón
Published: May 2022

Ensuring equitable and inclusive access to educational services in Higher Education Institutions (HEIs) requires the development of strategies that consider the diversity of their academic members, administrative staff, and students, as well as the use of information and communication technologies. The identification of requirements for technological accessibility in HEIs allows for the establishment of actions aimed at considering accessibility aspects in the processes of admission, permanence, and graduation, in order to support students with disabilities in their transit through these institutions. Having a systematic approach to guide the design of educational strategies in HEIs contributes to the identification of areas for improvement for the benefit of educational quality and community members. This article describes the proposal of a process based on the Plan-Do-Check-Act (PDCA) cycle, and a methodology for the implementation of accessible learning environments oriented to the implementation of an accessible virtual campus based on the establishment of five defined phases: diagnosis, planning, implementation, control, and tracing. This proposal is aimed at supporting Latin American HEIs in the integration of technological accessibility requirements from a systematic and continuous improvement approach.
Contactless Soil Moisture Mapping Using Inexpensive Frequency-Modulated Continuous Wave RADAR for Agricultural Purposes
Authors: Coutinho Rui M. , Sousa Armando , Santos Filipe , Cunha Mário
Published: May 2022

Soil Moisture (SM) is one of the most critical factors for a crop’s growth, yield, and quality. Although Ground-Penetrating RADAR (GPR) is commonly used in satelite observation to analyze soil moisture, it is not cost-effective for agricultural applications. Automotive RADAR uses the concept of Frequency-Modulated Continuous Wave (FMCW) and is more competitive in terms of price. This paper evaluates the viability of using a cost-effective RADAR as a substitute for GPR for soil moisture content estimation. The research consisted of four experiments, and the results show that the RADAR’s output signal and the soil moisture sensor SEN0193 have a high correlation with values as high as 0.93 when the SM is below 15%. Such results show that the tested sensor (and its cost-effective working principle) are able to determine soil water content (with certain limitations) in a non-intrusive, proximal sensing manner.
Self-Supervised Sentiment Analysis in Spanish to Understand the University Narrative of the Colombian Conflict
Authors: Rendón-Cardona Paula , Gil-Gonzalez Julian , Páez-Valdez Julián , Rivera-Henao Mauricio
Published: May 2022

Sentiment analysis is a relevant area in the natural language processing context–(NLP) that allows extracting opinions about different topics such as customer service and political elections. Sentiment analysis is usually carried out through supervised learning approaches and using labeled data. However, obtaining such labels is generally expensive or even infeasible. The above problems can be faced by using models based on self-supervised learning, which aims to deal with various machine learning paradigms in the absence of labels. Accordingly, we propose a self-supervised approach for sentiment analysis in Spanish that comprises a lexicon-based method and a supervised classifier. We test our proposal over three corpora; the first two are labeled datasets, namely, CorpusCine and PaperReviews. Further, we use an unlabeled corpus conformed by news related to the Colombian conflict to understand the university journalistic narrative of the war in Colombia. Obtained results demonstrate that our proposal can deal with sentiment analysis settings in scenarios with unlabeled corpus; in fact, it acquires competitive performance compared with state-of-the-art techniques in partially-labeled datasets.
A Review of Recent Literature on Systems and Methods for the Control of Thermal Comfort in Buildings
Authors: Grassi Benedetta , Piana Edoardo Alessio , Lezzi Adriano Maria , Pilotelli Mariagrazia
Published: May 2022

Thermal comfort in indoor environments is perceived as an important factor for the well-being and productivity of the occupants. To practically create a comfortable environment, a combination of models, systems, and procedures must be applied. This systematic review collects recent studies proposing complete thermal-comfort-based control strategies, extracted from a scientific database for the period 2017–2021. The study consists of this paper and of a spreadsheet recording all the 166 reviewed works. After a general introduction, the content of the papers is analyzed in terms of thermal comfort models, indoor environment control strategies, and correlation between these two aspects. Practical considerations on scope, required inputs, level of readiness, and, where available, estimated cost are also given. It was found that the predicted mean vote is the preferred thermal comfort modeling approach, followed by data-driven and adaptive methods. Thermal comfort is controlled mainly through indoor temperature, although a wide range of options are explored, including the comfort-based design of building elements. The most popular field of application of advanced control strategies is office/commercial buildings with air conditioning systems, which can be explained by budget and impact considerations. The analysis showed that few works envisaging practical implementations exist that address the needs of vulnerable people. A section is, therefore, dedicated to this issue.
A Comparison of Turning Kinematics at Different Amplitudes during Standing Turns between Older and Younger Adults
Authors: Khobkhun Fuengfa , Hollands Mark , Richards Jim
Published: May 2022

It is well-established that processes involving changing direction or turning in which either or both standing and walking turns are utilized involve coordination of the whole-body and stepping characteristics. However, the turn context and whole-body coordination have not been fully explored during different turning amplitudes. For these reasons, this present study aimed to determine the effects of turning amplitude on whole-body coordination. The findings from this study can be utilized to inform the rationale behind fall prevention factors and to help design an exercise strategy to address issues related to amplitude of turning in older adults. Twenty healthy older and twenty healthy younger adults were asked to complete standing turns on level ground using three randomly selected amplitudes, 90°, 135° and 180°, at their self-selected turn speed. Turning kinematics and stepping variables were recorded using Inertial Measurement Units. Analysis of the data was carried out using Mixed Model Analysis of Variance with two factors (2 groups × 3 turning amplitudes) and further post hoc pairwise analysis to examine differences between factors. There were significant interaction effects (p < 0.05) between the groups and turning amplitudes for step duration and turn speed. Further analysis using Repeated Measure Analysis of Variance tests determined a main effect of amplitude on step duration and turn speed within each group. Furthermore, post hoc pairwise comparisons revealed that the step duration and turn speed increased significantly (p < 0.001) with all increases in turning amplitude in both groups. In addition, significant main effects for group and amplitudes were seen for onset latency of movement for the head, thorax, pelvis, and feet, and for peak head–thorax and peak head–pelvis angular separations and stepping characteristics, which all increased with turn amplitude and showed differences between groups. These results suggest that large amplitude turns result in a change in turning and stepping kinematics. Therefore, when assessing the turning characteristics of older adults or those in frail populations, the turning amplitude should be taken into account during turning, and could be gradually increased to challenge motor control as part of exercise falls prevention strategies.
Modelling and Analysis of Viscoelastic and Nanofluid Effects on the Heat Transfer Characteristics in a Double-Pipe Counter-Flow Heat Exchanger
Authors: Mavi Anele , Chinyoka Tiri , Gill Andrew
Published: May 2022

This study computationally investigates the heat transfer characteristics in a double-pipe counter-flow heat-exchanger. A heated viscoelastic fluid occupies the inner core region, and the outer annulus is filled with a colder Newtonian-Fluid-Based Nanofluid (NFBN). A mathematical model is developed to study the conjugate heat transfer characteristics and heat exchange properties from the hot viscoelastic fluid to the colder NFBN. The mathematical modelling and formulation of the given problem comprises of a system of coupled nonlinear partial differential Equations (PDEs) governing the flow, heat transfer, and stress characteristics. The viscoelastic stress behaviour of the core fluid is modelled via the Giesekus constitutive equations. The mathematical complexity arising from the coupled system of transient and nonlinear PDEs makes them analytically intractable, and hence, a recourse to numerical and computational methodologies is unavoidable. A numerical methodology based on the finite volume methods (FVM) is employed. The FVM algorithms are computationally implemented on the OpenFOAM software platform. The dependence of the field variables, namely the velocity, temperature, pressure, and polymeric stresses on the embedded flow parameters, are explored in detail. In particular, the results illustrate that an increase in the nanoparticle volume-fraction, in the NFBN, leads to enhanced heat-exchange characteristics from the hot core fluid to the colder shell NFBN. Specifically, the results illustrate that the use of NFBN as the coolant fluid leads to enhanced cooling of the hot core-fluid as compared to using an ordinary (nanoparticle free) Newtonian coolant.
The Vehicle-to-Grid Concept with Respect to the Preferences of Electric Vehicle Drivers and Charging Station Operators
Authors: Kajanova Martina , Bracinik Peter
Published: May 2022

Recent events in Europe show the necessity of speeding up the transition to local renewable energy sources utilization within countries’ power systems. Moreover, the share of electric vehicles is gradually increasing within the Europe market. To utilize renewable energy sources with intermittent character to supply power for this increasing demand for electric vehicle charging, the vehicle-to-grid concept must be applied. However, for this concept to be successful, it should be beneficial for electric vehicle drivers and charging station operators. As a result, the focus of this research is on developing a charging/discharging control algorithm that adheres to the above assumptions. The use of the proposed control mechanism in case studies demonstrates that if electric vehicle drivers declare their preferences truthfully, the social welfare of all participants can be maximized. To prevent one of the drivers from trying to disrupt the social welfare concept in their favor by reporting false preferences, the incentive mechanism is proposed as well. The case studies show that the incentive mechanism results in the increased cost of the participant trying to disrupt the social welfare while decreasing the cost of all the others. Therefore, truth-telling and thus maximizing the social welfare of all participants in the system is an optimal strategy for each of them.
Assuring Anonymity and Privacy in Electronic Voting with Distributed Technologies Based on Blockchain
Authors: Neziri Vehbi , Shabani Isak , Dervishi Ramadan , Rexha Blerim
Published: May 2022

Anonymity and privacy in the electoral process are mandatory features found in any democratic society, and many authors consider these fundamental civil liberties and rights. During the election process, every voter must be identified as eligible, but after casting a vote, the voter must stay anonymous, assuring voter and vote unlinkability. Voter anonymity and privacy are the most critical issues and challenges of almost all electronic voting systems. However, vote immutability must be assured as well, which is a problem in many new democracies, and Blockchain as a distributed technology meets this data immutability requirement. Our paper analyzes current solutions in Blockchain and proposes a new approach through the combination of two different Blockchains to achieve privacy and anonymity. The first Blockchain will be used for key management, while the second will store anonymous votes. The encrypted vote is salted with a nonce, hashed, and finally digitally signed with the voter’s private key, and by mixing the timestamp of votes and shuffling the order of cast votes, the chances of linking the vote to the voter will be reduced. Adopting this approach with Blockchain technology will significantly transform the current voting process by guaranteeing anonymity and privacy.
Effect of Coir Fiber Reinforcement on Properties of Metakaolin-Based Geopolymer Composite
Authors: Ayeni Olugbenga , Mahamat Assia Aboubakar , Bih Numfor Linda , Stanislas Tido Tiwa , Isah Ibrahim , Savastano Junior Holmer , Boakye Emmanuel , Onwualu Azikiwe Peter
Published: May 2022

This study explored the use of coir fibers extracted from coconut husks, an agro-waste material that constitutes sanitation and environmental pollution problems, as a reinforcing element in the production of metakaolin-based geopolymer composites with improved properties. A series of sample formulations were produced with varying coir fiber content (0.5, 1.0, 1.5, and 2.0 percent weight of metakaolin powder). The investigation was conducted using a 10 M NaOH alkaline solution with a 0.24 NaOH:Na2SiO3 mass ratio. Samples were cured for 28 days and tested for bulk density, ultrasonic pulse velocity (UPV), and compressive and flexural strength. Microstructural examinations such as X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM) were also performed on samples. Compressive strength values up to 21.25 N/mm2 at 0.5% fiber content and flexural strength values up to 10.39 N/mm2 at 1% fiber content were achieved in this study. The results obtained showed a decreasing bulk density of geopolymer samples (2113 kg/m3 to 2045 kg/m3) with increasing coir fiber content. The geopolymer samples had UPV values varying from 2315 m/s to 2717 m/s. Coir fiber with 0.5–1.0% fiber content can be incorporated into metakaolin-based geopolymers to produce eco-friendly composite materials with improved mechanical properties for sustainable development.
Numerical Analysis of Ground Settlement Patterns Resulting from Tunnel Excavation in Composite Strata
Authors: Deng Huijuan , Cao Ping , Liu Yinzhu , Liu Zhizhen , Meng Gang , Fan Zhi , Xie Weiping
Published: May 2022

Cross-river twin tunnels are prone to deformation and uneven settlement of the surrounding soil due to the complexity of the strata crossed, which has a negative impact on the tunnel structure. A numerical calculation model was established using the COMSOL Multiphysics to study the effects of twin tunnel excavation in composite strata on the ground settlement and the ground settlement pattern. The results indicated that after the construction of the twin tunnels is completed, the ground settlement above the first tunnel is slightly larger than the ground settlement above the second tunnel. The further the spacing between the two tunnels before and after excavation, the smaller the amount of ground settlement and the impact on the surrounding soil. The ground settlement value increases with the increase in burial depth, and the ground settlement curve gradually changes from a W-shaped curve to a V-shaped curve. As the distance between the two tunnels increases, the maximum settlement value of the settlement curve gradually decreases, and the ground lateral settlement curve changes from V-shaped to W-shaped. The higher the water level on the riverbed side, the greater the settlement value of the ground.
High-Performance Concrete Nanomodified with Recycled Rice Straw Biochar
Authors: Beskopylny Alexey N. , Stel’makh Sergey A. , Shcherban’ Evgenii M. , Mailyan Levon R. , Meskhi Besarion , Smolyanichenko Alla S. , Beskopylny Nikita
Published: May 2022

The development of new and improvement of existing technologies based on the use of waste products from various industries or recyclable materials is a current trend in the construction industry. Including in the composition of binders and concrete by-products of industry, reducing the proportion of Portland cement, it is crucial to maintain and improve the resulting products’ mechanical characteristics and life cycle. The main aim of the study was to investigate the influence of biochar additive on the microstructure and properties of the concrete and obtain the composition with improved characteristics due to nanomodification of rice straw recycled biochar. An environmentally friendly technology for concrete manufacture was obtained, using agricultural waste, rice straw, as its components, developing a composition of concrete nanomodified with processed rice straw biochar, identifying the dependences of concrete properties on their nanomodification with processed rice straw coal. It has been established that the most effective dosage is the addition of rice straw biochar in the amount of 6% by weight of cement. The improvement in the properties of concrete was expressed in the increase in its physical and mechanical characteristics and changes in deformability according to the results of the analysis of the stress-strain diagrams. The increase in strength characteristics ranged from 17% to 25%. The modulus of elasticity increased to 14%. The deformation characteristics decreased from 12% to 24%. Introducing a finely dispersed additive of rice straw biochar modified by the electromagnetic method leads to a decrease in cement consumption by up to 10%.
Numerical Analysis for Ground Subsidence Caused by Extraction Holes of Removed Piles
Authors: Inazumi Shinya , Kuwahara Shuichi , Nontananandh Supakij , Jotisankasa Apiniti , Chaiprakaikeow Susit
Published: May 2022

Around the world, and especially in Japan, the tearing down of social infrastructure, including civil engineering structures, has been increasing due to the aging of these constructions, which were built during a period of high economic growth, and a decrease in their utilization caused by a recent drop in the population. The number of existing pile foundations being pulled out has gradually risen to a higher number than that of pile foundations being newly installed. However, after the pulling-out of a pile foundation, the mechanical characteristics of the surrounding ground are of great concern due to the existence of the holes that form when the existing piles are removed (extraction holes). In this study, a three-dimensional elasto-plastic consolidation analysis was performed to examine the effect of the extraction holes of removed piles on the static properties of the surrounding ground. As examples of the results of the analysis, if an extraction hole of a removed pile is left as it is, large ground subsidence will occur near the extraction hole of removed pile, especially at the lower part of the clay layer near the hole. The greater the number of extraction holes of removed piles, the greater the compressive stress acting on the extraction holes after the pile removal. Therefore, the filler should exhibit strength early as the number of extraction holes of removed piles increases.
Response Surface Methodological Approach for Optimizing Theobroma cacao L. Oil Extraction
Authors: Saelee Manee , Sivamaruthi Bhagavathi Sundaram , Tansrisook Chawin , Duangsri Sasiwimon , Chaiyasut Khontaros , Kesika Periyanaina , Peerajan Sartjin , Chaiyasut Chaiyavat
Published: May 2022

Theobroma cacao L. (Cocoa) is an agricultural product that is economically valuable worldwide; it is rich in bioactive compounds such as phenolic compounds and flavonoids. These compounds are known for their anti-inflammatory, anticarcinogenic, antimicrobial, antiulcer, and immune-modulating properties. Cocoa powder and cocoa butter are the major cocoa seed products, and cocoa seed oil (CSO) is the least-studied cocoa seed product. CSO is used in several industries; therefore, optimizing the extraction of high-quality CSO is essential. We used response surface methodology (RSM) to optimize the restriction dies, temperature, and sieve size to achieve a high yield and quality of CSO. The quality of the CSO was assessed according to total phenolic content (TPC), acid, and peroxide values, fatty acid content, and nitric oxide free radical scavenging activity. The highest yield (actual value: 46.10%; predicted value: 45.82%) was observed with the following restriction parameters: die size: 0.8 cm, temperature: 40 °C, and sieve size > 1.4 mm. The 2FI model for CSO extraction, the pressing time, the reduced quadratic model for acid value, the reduced cubic model for peroxide value, and the TPC showed that the model was significant. Our study primarily reported the impact of sieve size, restriction die, and temperature on CSO yield, acid, peroxide values, TPC of the CSO, and the influence of pressing time on the quantity and quality of the CSO. The high yield of CSO was of relatively lower quality. The temperature affected the yield, acid, peroxide values, TPC, and the nitric oxide free radical scavenging activity. In comparison, the fatty acid composition of the CSO was not affected by the processing temperature or sieve size. The results indicated that the extraction conditions must be chosen based on the application of the extracted oil. Further studies are warranted to confirm the results and further analyze other influential parameters during CSO extraction.
FSM-HSVM-Based Locomotion Mode Recognition for Exoskeleton Robot
Authors: Qi Zhuo , Song Qiuzhi , Liu Yali , Guo Chaoyue
Published: May 2022

This paper proposes a hierarchical support vector machine recognition algorithm based on a finite state machine (FSM-HSVM) to accurately and reliably recognize the locomotion mode recognition of an exoskeleton robot. As input signals, this method utilizes the angle information of the hip joint and knee joint collected by inertial sensing units (IMUs) on the thighs and shanks of the exoskeleton and the plantar pressure information collected by force sensitive resistors (FSRs) are used as input signals. This method establishes a framework for mode transition by combining the finite state machine (FSM) with the common locomotion modes. The hierarchical support vector machine (HSVM) recognition model is then tightly integrated with the mode transition framework to recognize five typical locomotion modes and eight locomotion mode transitions in real-time. The algorithm not only reduces the abrupt change in the recognition of locomotion mode, but also significantly improves the recognition efficiency. To evaluate recognition performance, separate experiments are conducted on six subjects. According to the results, the average accuracy of all motion modes is 97.106% ± 0.955%, and the average recognition delay rate is only 25.017% ± 6.074%. This method has the benefits of a small calculation amount and high recognition efficiency, and it can be applied extensively in the field of robotics.