bluuebunny/crossref_metadata_2025 huggingface dataset:
Hantavirus Infections among Military Forces
Authors: Mustonen Jukka , Henttonen Heikki , Vaheri Antti
Published: July 2023

Introduction

Hantaviruses cause two kinds of clinical syndromes. Hemorrhagic fever with renal syndrome is caused by Hantaan virus in Asia, Puumala virus (PUUV) and Dobrava virus in Europe, and Seoul virus worldwide. Hantavirus cardiopulmonary syndrome is caused by Sin Nombre virus in North America and Andes virus and related viruses in Latin America. All hantaviruses are carried by rodents and insectivores. Humans are infected via inhaled aerosols of rodent excreta. In the history, there are several epidemics of acute infectious diseases during many wars, which have been suggested or proven to be caused by various hantaviruses.

Materials and Methods

Literature review of 41 original publications and reviews published between 1943 and 2022 was performed. Among them, 23 publications handle hantavirus infections among military forces, and the rest 17 hantavirus infections themselves.

Results

A large epidemic during World War II in 1942 among German and Finnish soldiers in Northern Finland with more than 1,000 patients was most probably caused by PUUV. During Korean War in 1951–1954,∼ 3,200 cases occurred among United Nations soldiers in an epidemic caused by Hantaan virus. During Balkan war from 1991 to 1995, numerous soldiers got ill because of hantavirus infection caused by PUUV and Dobrava virus. Several other reports of cases of various hantavirus infections especially among U.S. soldiers acting in South Korea, Germany, Bosnia, and Kosovo have been described in the literature.

Conclusions

Military maneuvers usually include soil removal, spreading, digging with accompanied dust, and living in field and other harsh conditions, which easily expose soldiers to rodents and their excreta. Therefore, the risks of hantavirus infections in military context are obvious. All military infections have been caused by hantaviruses leading to hemorrhagic fever with renal syndrome.
The Sum of Well-Being
Authors: Nebel Jacob M
Published: July 2023

Is well-being the kind of thing that can be summed across individuals? This paper takes a measurement-theoretic approach to answering this question. To make sense of adding well-being, we would need to identify some natural ‘concatenation’ operation on the bearers of well-being that satisfies the axioms of extensive measurement and can therefore be represented by the arithmetic operation of addition. I explore various proposals along these lines, involving the concatenation of segments within lives over time, of entire lives led alongside one another or in sequence, and of evaluatively basic propositions via conjunction. All of these proposals turn out to carry highly controversial commitments about the good. I do not claim that these commitments are unacceptable. But they suggest that we cannot simply take for granted, as many philosophers do, that there is any such thing as the sum of well-being.
Reconstruction of inflationary scenarios in non-conservative unimodular gravity
Authors: Piccirilli María Pía , León Gabriel
Published: July 2023

Unimodular gravity is an alternative theory of gravity to general relativity. The gravitational field equations are given by the trace-free version of Einstein’s field equations. Due to the structure of the theory, unimodular gravity admits a diffusion term that characterizes a possible non-conservation of the canonical energy–momentum tensor locally. Employing this feature of unimodular gravity, in this work, we explicitly show how to construct an inflationary phase that can be contrasted with current observations. In particular, we focus on three different inflationary scenarios of physical interest. An important element in these scenarios is that the accelerated expansion is driven by the diffusion term exclusively, i.e. there is no inflaton. Furthermore, the primordial spectrum during inflation is generated by considering inhomogeneous perturbations associated to standard hydrodynamical matter (modelled as a single ultra-relativistic fluid). For each of the scenarios, we obtain the prediction for the primordial spectrum and contrast it with recent observational bounds using Planck 2018 + Bicep2/KECK Array data.
Brain metastasis screening in the molecular age
Authors: Tabor Joanna K , Onoichenco Amanda , Narayan Vinayak , Wernicke A Gabriella , D’Amico Randy S , Vojnic Morana
Published: July 2023

The incidence of brain metastases (BM) amongst cancer patients has been increasing due to improvements in therapeutic options and an increase in overall survival. Molecular characterization of tumors has provided insights into the biology and oncogenic drivers of BM and molecular subtype-based screening. Though there are currently some screening and surveillance guidelines for BM, they remain limited. In this comprehensive review, we review and present epidemiological data on BM, their molecular characterization, and current screening guidelines. The molecular subtypes with the highest BM incidence are epithelial growth factor receptor-mutated non-small cell lung cancer (NSCLC), BRCA1, triple-negative (TN), and HER2+ breast cancers, and BRAF-mutated melanoma. Furthermore, BMs are more likely to present asymptomatically at diagnosis in oncogene-addicted NSCLC and BRAF-mutated melanoma. European screening standards recommend more frequent screening for oncogene-addicted NSCLC patients, and clinical trials are investigating screening for BM in hormone receptor+, HER2+, and TN breast cancers. However, more work is needed to determine optimal screening guidelines for other primary cancer molecular subtypes. With the advent of personalized medicine, molecular characterization of tumors has revolutionized the landscape of cancer treatment and prognostication. Incorporating molecular characterization into BM screening guidelines may allow physicians to better identify patients at high risk for BM development and improve patient outcomes.
High-dose oral vitamin D supplementation for prevention of infections in children aged 0 to 59 months: a systematic review and meta-analysis
Authors: Carboo Janet Adede , Dolman-Macleod Robin Claire , Malan Linda , Lombard Martani Johanni
Published: July 2023

Context

Vitamin D plays an important role in immune function, and the deficiency thereof has been associated with several infections, most notably respiratory tract infections. However, data from intervention studies investigating the effect of high-dose vitamin D supplementation on infections have been inconclusive.

Objective

The aim of this study was to evaluate the level of evidence regarding the efficacy of vitamin D supplementation above the standard dose (400 IU) in preventing infections in apparently healthy children < 5 years of age.

Data Sources

PubMed, Scopus, Science Direct, Web of Science, Google Scholar, CINAHL, and MEDLINE electronic databases were searched between August 2022 and November 2022. Seven studies met the inclusion criteria.

Data Extraction

Meta-analyses of outcomes in more than one study were performed using Review Manager software. Heterogeneity was evaluated using the I2 statistic. Randomized controlled trials in which vitamin D was supplemented at > 400 IU compared with placebo, no treatment, or standard dose were included.

Data Analysis

Seven trials that enrolled a total of 5748 children were included. Odds ratios (ORs) with 95%CIs were calculated using random- and fixed-effects models. There was no significant effect of high-dose vitamin D supplementation on the incidence of upper respiratory tract infection (OR, 0.83; 95%CI, 0.62–1.10). There was a 57% (95%CI, 0.30–0.61), 56% (95%CI, 0.27–0.07), and 59% (95%CI, 0.26–0.65) reduction in the odds of influenza/cold, cough, and fever incidence, respectively, with daily supplementation of vitamin D > 1000 IU. No effect was found on bronchitis, otitis media, diarrhea/gastroenteritis, primary care visits for infections, hospitalizations, or mortality.

Conclusion

High-dose vitamin D supplementation provided no benefit in preventing upper respiratory tract infections (moderate certainty of evidence) but reduced the incidence influenza/cold (moderate certainty of evidence), cough, and fever (low certainty of evidence). These findings are based on a limited number of trials and should be interpreted with caution. Further research is needed.

Systematic Review Registration

PROSPERO registration number CRD42022355206.
Prevalence ofKRASG12C Mutation and Co-mutations and Associated Clinical Outcomes in Patients With Colorectal Cancer: A Systematic Literature Review
Authors: Strickler John H , Yoshino Takayuki , Stevinson Kendall , Eichinger Christian Stefan , Giannopoulou Christina , Rehn Marko , Modest Dominik Paul
Published: July 2023

Purpose

A systematic literature review was conducted to estimate the global prevalence of Kirsten rat sarcoma virus gene (KRAS) mutations, with an emphasis on the clinically significant KRAS G12C mutation, and to estimate the prognostic significance of these mutations in patients with colorectal cancer (CRC).

Design

Relevant English-language publications in the Embase, MEDLINE, and the Cochrane Library databases (from 2009 to 2021) and congress presentations (from 2016 to 2021) were reviewed. Eligible studies were those that reported the prevalence and clinical outcomes of the KRAS G12C mutation in patients with CRC.

Results

A total of 137 studies (interventional [n = 8], post hoc analyses of randomized clinical trials [n = 6], observational [n = 122], and longitudinal [n =1]) were reviewed. Sixty-eight studies reported the prevalence of KRAS mutations (KRASm) in 42 810 patients with CRC. The median global prevalence of KRASm was 38% (range, 13.3%-58.9%) and that of the KRAS G12C mutation (KRAS G12C) 3.1% (range, 0.7%-14%). Available evidence suggests that KRASm are possibly more common in tumors that develop on the right side of the colon. Limited evidence suggests a lower objective response rate and inferior disease-free/relapse-free survival in patients with KRAS G12C compared with patients with KRASwt or other KRASm.

Conclusion

Our analysis reveals that KRAS G12C is prevalent in 3% of patients with CRC. Available evidence suggests a poor prognosis for patients with KRAS G12C. Right-sided tumors were more likely to harbor KRASm; however, their role in determining clinical outcomes needs to be investigated further.
Clinical and Economic Outcomes in Patients With Metastatic Urothelial Carcinoma Receiving First-Line Systemic Treatment (the IMPACT UC I Study)
Authors: Bilen Mehmet A , Robinson Scott B , Schroeder Amy , Peng Jing , Kim Ruth , Liu Frank X , Bhanegaonkar Abhijeet
Published: July 2023

Background

The IMPACT UC I study assessed real-world treatment patterns, outcomes, healthcare resource utilization (HCRU), and costs in patients with metastatic urothelial carcinoma (mUC) receiving first-line (1L) systemic treatment after the FDA approval of 1L immune checkpoint inhibitor (ICI) monotherapy.

Patients and Methods

This retrospective study used 100% Medicare fee-for-service claims from 1/1/2015 to 6/30/2019 to identify patients aged ≥18 years diagnosed with UC with evidence of metastatic disease, continuously enrolled for 6 months before and after initial diagnosis. Patients were grouped by 1L treatment: cisplatin-containing chemotherapy, carboplatin-containing chemotherapy, ICI monotherapy, or nonplatinum-containing therapy. Unadjusted time on 1L treatment (TOT), overall survival (OS), HCRU, and total healthcare costs were analyzed.

Results

Of 18 888 patients with mUC, 8630 (45.7%) had received identified 1L systemic treatment; platinum-containing chemotherapy was the most common (cisplatin-containing chemotherapy, 37.6%; carboplatin-containing chemotherapy, 30.2%). Cisplatin- and carboplatin-containing chemotherapy had the shortest time-to-treatment initiation (median, 1.7-3.0 months) and longest TOT (median, 4.0-4.3 months). Median OS was longest with cisplatin-containing chemotherapy (20.0 months) and shortest with ICI monotherapy (7.6 months). Cisplatin- and carboplatin-containing chemotherapy were associated with highest HCRU; total healthcare costs were approximately 2-fold higher with ICI monotherapy vs other 1L treatments ($10 359 vs $5042-$5709 per patient per month).

Conclusion

1L platinum-containing chemotherapy resulted in the longest median OS and highest HCRU, whereas 1L ICI treatment had the shortest median OS and the highest costs. Over 50% of patients diagnosed with advanced UC (aUC) received no systemic therapy, highlighting the importance of optimal 1L treatment decisions in aUC.
G-type receptor-like kinase AsNIP43 interacts with rhizobia effector nodulation outer protein P and is required for symbiosis
Authors: Liu Yuan , Lin Ye , Wei Feng , Lv Yanfei , Xie Fuli , Chen Dasong , Lin Hui , Li Youguo
Published: July 2023

In the Rhizobium-Legume symbiosis, the nodulation outer protein P (NopP) effector is one of the key regulators for rhizobial infection and nodule organogenesis. However, the molecular mechanism through which host legume plants sense NopP remains largely unknown. Here, we constructed an nopP deletion mutant of Mesorhizobium huakuii and found that nopP negatively regulates nodulation on Chinese milk vetch (Astragalus sinicus). Screening for NopP interacting proteins in host plants using the yeast 2-hybrid system identified NopP interacting protein 43 (AsNIP43), which encodes a G-type receptor-like kinase (LecRLK). The B-lectin domain at the N terminus of AsNIP43 was essential in mediating its interaction with NopP, which was confirmed in vitro and in vivo. Subcellular localization, co-localization, and gene expression analyses showed that AsNIP43 and NopP function tightly associated with earlier infection events. RNA interference (RNAi) knockdown of AsNIP43 expression by hairy root transformation led to decreased nodule formation. AsNIP43 plays a positive role in symbiosis, which was further verified in the model legume Medicago truncatula. Transcriptome analysis indicated that MtRLK (a homolog of AsNIP43 in M. truncatula) may function to affect defense gene expression and thus to regulate early nodulation. Taken together, we show that LecRLK AsNIP43 is a legume host target that interacts with rhizobia effector NopP is essential for rhizobial infection and nodulation.
A truncated ETHYLENE INSENSITIVE3-like protein, GhLYI, regulates senescence in cotton
Authors: Zhang Yayao , Zang Yihao , Chen Jinwen , Feng Shouli , Zhang Zhiyuan , Hu Yan , Zhang Tianzhen
Published: July 2023

Numerous endogenous and environmental signals regulate the intricate and highly orchestrated process of plant senescence. Ethylene (ET), which accumulates as senescence progresses, is a major promoter of leaf senescence. The master transcription activator ETHYLENE INSENSITIVE3 (EIN3) activates the expression of a wide range of downstream genes during leaf senescence. Here, we found that a unique EIN3-LIKE 1 (EIL1) gene, cotton LINT YIELD INCREASING (GhLYI), encodes a truncated EIN3 protein in upland cotton (Gossypium hirsutum L.) that functions as an ET signal response factor and a positive regulator of senescence. Ectopic expression or overexpression of GhLYI accelerated leaf senescence in both Arabidopsis (Arabidopsis thaliana) and cotton. Cleavage under targets and tagmentation (CUT&Tag) analyses revealed that SENESCENCE-ASSOCIATED GENE 20 (SAG20) was a target of GhLYI. Electrophoretic mobility shift assay (EMSA), yeast 1-hybrid (Y1H), and dual-luciferase transient expression assay confirmed that GhLYI directly bound the promoter of SAG20 to activate its expression. Transcriptome analysis revealed that transcript levels of a series of senescence-related genes, SAG12, NAC-LIKE, ACTIVATED by APETALA 3/PISTILLATA (NAP/ANAC029), and WRKY53, are substantially induced in GhLYI overexpression plants compared with wild-type (WT) plants. Virus-induced gene silencing (VIGS) preliminarily confirmed that knockdown of GhSAG20 delayed leaf senescence. Collectively, our findings provide a regulatory module involving GhLYI-GhSAG20 in controlling senescence in cotton.
The apiosyltransferase celery UGT94AX1 catalyzes the biosynthesis of the flavone glycoside apiin
Authors: Yamashita Maho , Fujimori Tae , An Song , Iguchi Sho , Takenaka Yuto , Kajiura Hiroyuki , Yoshizawa Takuya , Matsumura Hiroyoshi , Kobayashi Masaru , Ono Eiichiro , Ishimizu Takeshi
Published: July 2023

Apiose is a unique branched-chain pentose found in plant glycosides and a key component of the cell wall polysaccharide pectin and other specialized metabolites. More than 1,200 plant-specialized metabolites contain apiose residues, represented by apiin, a distinctive flavone glycoside found in celery (Apium graveolens) and parsley (Petroselinum crispum) in the family Apiaceae. The physiological functions of apiin remain obscure, partly due to our lack of knowledge on apiosyltransferase during apiin biosynthesis. Here, we identified UGT94AX1 as an A. graveolens apiosyltransferase (AgApiT) responsible for catalyzing the last sugar modification step in apiin biosynthesis. AgApiT showed strict substrate specificity for the sugar donor, UDP-apiose, and moderate specificity for acceptor substrates, thereby producing various apiose-containing flavone glycosides in celery. Homology modeling of AgApiT with UDP-apiose, followed by site-directed mutagenesis experiments, identified unique Ile139, Phe140, and Leu356 residues in AgApiT, which are seemingly crucial for the recognition of UDP-apiose in the sugar donor pocket. Sequence comparison and molecular phylogenetic analysis of celery glycosyltransferases suggested that AgApiT is the sole apiosyltransferase-encoding gene in the celery genome. Identification of this plant apiosyltransferase gene will enhance our understanding of the physioecological functions of apiose and apiose-containing compounds.
Gene–environment interactions and the case of body mass index and obesity: How much do they matter?
Authors: Huangfu Yiyue , Palloni Alberto , Beltrán-Sánchez Hiram , McEniry Mary C
Published: July 2023

We investigate the demographic and population health implications of gene–environment interactions (GxE) in the case of body mass index (BMI) and obesity. We seek to answer two questions: (a) what is the first-order impact of GxE effects on BMI and probability of obesity, e.g. the direct causal effect of G in different E's? and (b) how large is the impact of GxE effects on second-order health outcomes associated with BMI and obesity, such as type 2 diabetes (T2D) and disability? In contrast to most of the literature that focuses on estimating GxE effects, we study the implications of GxE effects for population health outcomes that are downstream of a causal chain that includes the target phenotype (in this case BMI) as the initial cause. To limit the scope of the paper, we focus on environments defined by birth cohorts. However, extensions to other environments (education, socioeconomic status (SES), early conditions, and physical settings) are straightforward.
Heterogeneity in network structure switches the dominant transmission mode of infectious diseases
Authors: Kollepara Pratyush K , Chisholm Rebecca H , Miller Joel C
Published: July 2023

Several recent emerging diseases have exhibited both sexual and nonsexual transmission modes (Ebola, Zika, and mpox). In the recent mpox outbreaks, transmission through sexual contacts appears to be the dominant mode of transmission. Motivated by this, we use an SIR-like model to argue that an initially dominant sexual transmission mode can be overtaken by casual transmission at later stages, even if the basic casual reproduction number is less than one. Our results highlight the risk of intervention designs which are informed only by the early dynamics of the disease.
SUB1A-1 anchors a regulatory cascade for epigenetic and transcriptional controls of submergence tolerance in rice
Authors: Lin Chih-Cheng , Lee Wan-Jia , Zeng Cyong-Yu , Chou Mei-Yi , Lin Ting-Jhen , Lin Choun-Sea , Ho Meng-Chiao , Shih Ming-Che
Published: July 2023

Most rice (Oryza sativa) cultivars cannot survive under prolonged submergence. However, some O. sativa ssp. indica cultivars, such as FR13A, are highly tolerant owing to the SUBMERGENCE 1A-1 (SUB1A-1) allele, which encodes a Group VII ethylene-responsive factor (ERFVII) protein; other submergence-intolerant cultivars contain a SUB1A-2 allele. The two alleles differ only by a single substitution at the 186th amino acid position from serine in SUB1A-1 to proline in SUB1A-2 resulting in only SUB1A-1 being able to be phosphorylated. Two other ERFVIIs, ERF66 and ERF67, function downstream of SUB1A-1 to form a regulatory cascade in response to submergence stress. Here, we show that SUB1A-1, but not SUB1A-2, interacts with ADA2b of the ADA2b-GCN5 acetyltransferase complex, in which GCN5 functions as a histone acetyltransferase. Phosphorylation of SUB1A-1 at serine 186 enhances the interaction of SUB1A-1 with ADA2b. ADA2b and GCN5 expression was induced under submergence, suggesting that these two genes might play roles in response to submergence stress. In transient assays, binding of SUB1A-1 to the ERF67 promoter and ERF67 transcription were highly induced when SUB1A-1 was expressed together with the ADA2b-GCN5 acetyltransferase complex. Taken together, these results suggest that phospho-SUB1A-1 recruits the ADA2-GCN5 acetyltransferase complex to modify the chromatin structure of the ERF66/ERF67 promoter regions and activate gene expression, which in turn enhances rice submergence tolerance.
Promising by Normative Assurance
Authors: Passi Luca
Published: July 2023

This paper develops a new theory of the morality of promissory obligations. T. M. Scanlon notoriously argued that promising consists in assuring the promisee that we will do something. I disagree. I argue that it is true that promising consists in assuring the promisee, but what the promisor gives to the promisee is not an assurance that they will do something, but that the normative situation is in a certain way.
Physical Activity Behavior and Its Association With Global Cognitive Function Three Months After Stroke: The Nor-COAST Study
Authors: Luzum Geske , Gunnes Mari , Lydersen Stian , Saltvedt Ingvild , Tan Xiangchun , Thingstad Pernille , Thrane Gyrd , Askim Torunn
Published: July 2023

Objective

The purposes of this study were to determine the association between physical activity (PA) behavior and global cognitive function 3 months after stroke and to explore the role of physical capacity as a mediating factor.

Methods

Participants with stroke were successively recruited at 5 different hospitals in Norway. PA was measured using accelerometers, with a follow-up period of 7 consecutive days, and global cognitive function was assessed using the Montreal Cognitive Assessment (MoCA). The general pattern of PA and the percentage of participants adhering to World Health Organization PA recommendations (at least 150 minutes of moderate-intensity aerobic PA per week) were investigated using descriptive statistics. Multiple regression and mediator analyses were used to examine the relationship between PA behavior and MoCA scores; physical capacity, measured with the Short Physical Performance Battery, served as the mediating variable.

Results

A total of 193 women (42.6%) and 260 men (57.4%) with a median age of 73.7 years (25th and 75th percentiles = 65.8 and 80.4, respectively) and a median MoCA score of 25 points (25th and 75th percentiles = 22 and 27, respectively) were included. Mean total time spent walking at moderate intensity was 251.7 (SD = 164.6) min/wk (mean bout length = 20.9 [SD = 7.3] seconds), which indicated 69.3% adherence to World Health Organization guidelines. With each point decrease in the MoCA score, there was an expected 8.6% increase in the odds of nonadherence to PA recommendations. Physical capacity was identified as an important mediating factor, explaining the strength of the association between cognition and PA behavior.

Conclusions

In contrast to previous research, in the present study, most participants adhered to the updated global PA guidelines. However, people who had survived stroke and had reduced cognitive function were at higher risk of inactivity, an association mediated by physical capacity.

Impact

A better understanding of the association between cognition and PA behavior after stroke might help for developing more targeted early-onset interventions.
The Littleness of Little Dorrit
Authors: Weltman Sharon Aronofsky
Published: July 2023

This essay challenges prevailing interpretations of Amy Dorrit’s petiteness as a symbol of her abstemious self-sacrifice or of women’s infantilized position in Victorian culture. Examining Little Dorrit literally rather than symptomatically shows that Charles Dickens highlights the protagonist Amy’s small size on a spectrum of human variation, rendering in detail corporeal experiences in a physical world built for taller people. While the plentiful critical readings interpreting Amy as symbolic are illuminating, they depend on the unreliable points of view of other characters. Aware of others’ misperception of her in contrast to her understanding of herself, Amy develops a painful double consciousness that nonetheless gives her a capacity to see from more than one viewpoint. Shifting readers away from seeing Amy through the eyes of her misperceiving friends and family, Dickens critiques symptomatic reading and links Amy’s compassionate capacity for comprehending multiple perspectives to the omniscient narrator’s expansive point of view.
Dynamic Opinion Aggregation: Long-Run Stability and Disagreement
Authors: Cerreia-Vioglio Simone , Corrao Roberto , Lanzani Giacomo
Published: July 2023

This article proposes a model of non-Bayesian social learning in networks that accounts for heuristics and biases in opinion aggregation. The updating rules are represented by non-linear opinion aggregators from which we extract two extreme networks capturing strong and weak links. We provide graph-theoretic conditions for these networks that characterize opinions’ convergence, consensus formation, and efficient or biased information aggregation. Under these updating rules, agents may ignore some of their neighbours’ opinions, reducing the number of effective connections and inducing long-run disagreement for finite populations. For the wisdom of the crowd in large populations, we highlight a trade-off between how connected the society is and the non-linearity of the opinion aggregator. Our framework bridges several models and phenomena in the non-Bayesian social learning literature, thereby providing a unifying approach to the field.
Effects of feeding a vitamin and mineral supplement to cow-calf pairs grazing native range
Authors: Hurlbert Jennifer L , Baumgaertner Friederike , McCarthy Kacie L , Long Timothy , Wieland Cody , Sedivec Kevin K , Dahlen Carl R
Published: July 2023

Our objectives were to evaluate the impacts of providing vitamin and mineral (VTM) supplements to cow-calf pairs during the summer grazing period on cow and calf performance and liver concentrations of minerals. During a two-year period, 727 crossbred cows and their calves (initial cow BW = 601.7 ± 48.1 kg; calf BW = 87.8 ± 5.0 kg; n = 381 in year 1, n = 346 in year 2) from the Central Grasslands Research Extension Center (Streeter, N.D.) were blocked by parity (young [parity 1 to 3], and old [parity 4+]) and randomly assigned to pastures at the beginning of the grazing season (16 in year 1 and 14 in year 2). Pastures were assigned to receive a free-choice VTM supplement (SUPP) or no VTM supplement (CON) from pasture turnout to pasture removal (158 and 156 days in year 1 and 2, respectively). Consecutive day weights were taken from cows and calves at pasture turnout and removal and liver biopsies were collected from a subset of cows at both timepoints and from calves at weaning. Cows were bred via AI 37 to 41 d after pasture turnout and by natural service cleanup bulls for a 70 to 80 d breeding season. Calving and weaning data were collected from the calf conceived and gestated during treatments. Data were analyzed for the effect of VTM treatment (SUPP vs. CON), block of parity, and their interaction using the GLM procedure of SAS with pasture as the experimental unit. Year was considered a random effect in the final analysis. Cow pregnancy success was evaluated using the GLIMMIX procedure in SAS with model terms of VTM treatment, parity, and their interaction with year as a random effect. In year 2, cows in differing days postpartum (DPP) groups at pasture turnout (66.1, 48.8, and 34.5 ± 1.04 DPP for EARLY, MID, and LATE groups, respectively) were selected for liver biopsies with cow as the experimental unit. Cow and calf BW and BW change were not impacted (P ≥ 0.20) by VTM access. Pregnancy rate to AI, overall pregnancy rate, gestating calf birth BW and calving distribution were not affected (P ≥ 0.11) by treatment. Liver concentrations of Se, Cu, and Co were greater (P ≤ 0.002) at pasture removal and weaning for cows and suckling calves that had access to VTM. Cows considered EARLY calving had greater (P = 0.05) concentrations of liver Se compared with LATE calving cows. Although VTM supplementation enhanced concentrations of key minerals in the liver of cow-calf pairs, reproductive and growth performance was not affected.
A stomach plate to divide them all: a phylogenetic reassessment of the family Tritoniidae (Nudibranchia: Cladobranchia)
Authors: De Vasconcelos Silva Felipe , Pola Marta , Cervera Juan Lucas
Published: July 2023

The phylogeny of the family Tritoniidae has recently been studied with significant advances, but the relationships between genera are still controversial. In our study, we investigated the phylogeny of Tritoniidae using the most diverse taxon sampling possible. We applied an integrative approach based on new sequences of two mitochondrial genes (COI and 16S), a nuclear gene (H3) and morpho-anatomical characters. The monophyly of the family Tritoniidae was not recovered in our phylogenetic analyses. In view of our results, we propose a new rearrangement at the subfamily and genus levels. The plate-bearing genera are raised to the subfamily level as the monophyletic Marioniinae subfam. nov., formed by Marionia and the reinstated Marioniopsis. The remaining plate-less genera are raised to the subfamily level as the monophyletic Tritoniinae Lamarck, 1809. The genus Myrella is reinstated to assign the Antarctic and sub-Antarctic tritoniids. The taxonomic status of the monotypic Tritonidoxa is confirmed. Species delimitation analyses reveal a new large Marionia species from the Gulf of Cádiz (south-west Spain, Atlantic Ocean) and evidence for the pseudocryptic speciation of numerous Atlantic–Mediterranean and Indo-Pacific tritoniids. Finally, taxonomic notes are provided for several species according to the proposed rearrangement.
Osteological comparison of casque ontogeny in palaeognathous and neognathous birds: insights for selecting modern analogues in the study of cranial ornaments from extinct archosaurs
Authors: Green Todd L , Gignac Paul M
Published: July 2023

Birds, along with their dinosaurian precursors, possess a variety of bony cranial expansions. A deep understanding of the phenotypic complexity of these structures would be useful for addressing the development, evolution, and function of hard-tissue cranial ornamentation. Yet, the evolutionary significance and function of these structures have gone largely unaddressed because no unifying conceptual framework for interpreting bony cranial expansions currently exists. To provide such a framework, we examine osseous ornament variation in modern birds, using µ-CT imaging to examine the cranial casque components, structural composition, and developmental changes of two neognathous (Numida meleagris, Macrocephalon maleo) and one palaeognathous species (Casuarius casuarius) and survey the avian osteology literature of the 11 orders containing members with osseous cranial ornamentation. Our anatomical analyses suggest two broad configuration categories: (i) geminal, in which ornaments consist of paired elements only (i.e. within Neognathae) and (ii) disunited, in which ornaments consist of unpaired, midline elements along with paired bones (i.e. within Palaeognathae). Ornament bones contribute to casque elevation (proximal ornament support), elaboration (distal ornament shape), or both. Our results hold utility for unravelling the selection processes, particularly in difficult-to-decipher display roles, that shaped modern avian casques, as well as for the use of extant avians as comparative analogues of non-avian dinosaurs with ornamental head structures.
New information on the Eocene frog Thaumastosaurus (Anura, Pyxicephalidae) from the Phosphorites du Quercy, France
Authors: Georgalis Georgios L , Prendini Elizabeth , Roček Zbyněk
Published: July 2023

We present new disarticulated cranial elements of the Eocene frog Thaumastosaurus from several localities in the Phosphorites du Quercy, France, providing novel information about the variation of its anatomical characteristics. With the use of micro-computed tomography (μCT) scanning technology, we examine and discuss various types of dermal cranial ornamentation in extant Pyxicephalus, the closest extant relative of Thaumastosaurus, in which these features are useful in species diagnoses, paying particular attention to the individual and ontogenetic variation, and sexual dimorphism, as well as interspecific variation among extant species. We suggest that various types of dermal cranial ornamentation in Thaumastosaurus could be potentially used in diagnoses at species level, although ontogenetic variation should be taken into consideration. Apart from ornamentation, the size and general morphology of the maxillae and squamosa in Thaumastosaurus reveal an unexpected disparity of morphotypes, which suggests the potential presence of cryptic taxa. Some squamosa reveal that their processus zygomaticus extended up to the postnasal wall, so their associated maxillae were excluded from the orbital margin, whereas others were short and their respective maxillae participated in the formation of the orbital margin. Thaumastosaurus is envisaged as the product of an Early Palaeogene direct, potentially overseas, dispersal from isolated Afro-Arabia to Europe.
A new euharamiyidan, Mirusodens caii (Mammalia: Euharamiyida), from the Jurassic Yanliao Biota and evolution of allotherian mammals
Authors: Mao Fangyuan , Li Zhiheng , Hooker Jerry J , Meng Jin
Published: July 2023

Allotheria are an extinct group of mammaliaforms that originally comprised multituberculates, to which ‘haramiyidans’ and gondwanatherians were later added. Phylogenetic relationships of allotherians have remained controversial since the first reports of the allotherian fossils in the 1800s. Here we report a new euharamiyidan based on a skeletal specimen from the Jurassic Daohugou phase of the Yanliao Biota, China. The superb specimen preserves impressions of differentiated hairs. The pes with impressions of toe skin and claw sheath represents the first such evidence in Mesozoic mammaliamorphs. The remarkable tooth morphologies surpass in complexity any Mesozoic mammals previously known. Phylogenetic analyses place the new species within euharamiyidans from Eurasia and support euharamiyidans, multituberculates, and gondwanatherians as a tripartite monophyletic Allotheria within mammals. This clade is supported not only by their similar molar pattern, but also by many shared derived features of the dental system recognized from discoveries during the last two decades. Allotherians and therians represent the two most successful groups of mammals that are characterized by distinct dental systems. They originated at the latest in the Late Triassic and became diversified in the Middle Jurassic, representing two directions of ecomorphological adaptation during early evolution of mammals.
Effects of Altering Three Steps of Monolignol Biosynthesis on Sorghum Responses to Stalk Pathogens and Water Deficit
Authors: Funnell-Harris Deanna L. , Sattler Scott E. , O’Neill Patrick M. , Gries Tammy , Ge Zhengxiang , Nersesian Natalya
Published: July 2023

The drought-resilient crop sorghum (Sorghum bicolor [L.] Moench) is grown worldwide for multiple uses, including forage or potential lignocellulosic bioenergy feedstock. A major impediment to biomass yield and quality are the pathogens Fusarium thapsinum and Macrophomina phaseolina, which cause Fusarium stalk rot and charcoal rot, respectively. These fungi are more virulent with abiotic stresses such as drought. Monolignol biosynthesis plays a critical role in plant defense. The genes Brown midrib (Bmr)6, Bmr12, and Bmr2 encode the monolignol biosynthesis enzymes cinnamyl alcohol dehydrogenase, caffeic acid O-methyltransferase, and 4-coumarate:CoA ligase, respectively. Plant stalks from lines overexpressing these genes and containing bmr mutations were screened for pathogen responses with controlled adequate or deficit watering. Additionally, near-isogenic bmr12 and wild-type lines in five backgrounds were screened for response to F. thapsinum with adequate and deficit watering. All mutant and overexpression lines were no more susceptible than corresponding wild-type under both watering conditions. The bmr2 and bmr12 lines, near-isogenic to wild-type, had significantly shorter mean lesion lengths (were more resistant) than RTx430 wild-type when inoculated with F. thapsinum under water deficit. Additionally, bmr2 plants grown under water deficit had significantly smaller mean lesions when inoculated with M. phaseolina than under adequate-water conditions. When well-watered, bmr12 in cultivar Wheatland and one of two Bmr2 overexpression lines in RTx430 had shorter mean lesion lengths than corresponding wild-type lines. This research demonstrates that modifying monolignol biosynthesis for increased usability may not impair plant defenses but can even enhance resistance to stalk pathogens under drought conditions.
Impact of Fungicides on Dual-Purpose and Brown Midrib Zea mays Hybrids Used for Silage in Wisconsin
Authors: Chibuogwu Maxwell O. , Mueller Brian , Groves Carol L. , Smith Damon L.
Published: July 2023

Decisions about hybrid choice and fungicide application when growing corn ( Zea mays L.) for silage are important considerations to maximize sustainable production. The objective of this research was to evaluate how brown midrib hybrids compare with dual-purpose hybrids and how fungicides interact with these two classes of corn hybrids. To explore this, field research trials were conducted in Arlington, Wisconsin, in 2020 and 2021. In both years, the trials were arranged in a randomized complete block design and included one brown midrib hybrid, one dual-purpose hybrid, and seven fungicide application regimes. In-field disease ratings were made for tar spot (caused by Phyllachora maydis), ear rot, and stalk rot (caused by Gibberella zeae). Harvested silage was analyzed for yield, quality, and deoxynivalenol (DON) concentration. The selection of hybrid significantly ( P < 0.01) influenced the silage quality parameters, such as starch and total tract neutral detergent fiber digestibility, a measure of fiber digested throughout the tract of a cow. Fungicide use influenced dry matter yield ( P < 0.05), tar spot severity ( P < 0.01), and DON concentration ( P = 0.05). Our findings suggest that using appropriate fungicides improves yield and reduces disease severity and DON contamination. Better quality silage could be obtained from brown midrib hybrids at the expense of yield. Therefore, Wisconsin farmers are encouraged to primarily utilize a dual-purpose class of corn hybrids to ensure optimal milk production and secondarily apply fungicides to reduce DON levels in corn chopped for silage.
Population Genomics Reveals an Emerging Lineage of Xanthomonas perforans on Pepper
Authors: Subedi Aastha , Barrera Laura Belen Tapia de la , Ivey Melanie Lewis , Egel Daniel S. , Kebede Misrak , Kara Serhat , Aysan Yesim , Minsavage Gerald V. , Roberts Pamela D. , Jones Jeffrey B. , Goss Erica M.
Published: July 2023

Xanthomonas perforans—the dominant causal agent of bacterial leaf spot of tomato—is an emerging pathogen of pepper, indicative of a potential host expansion across the southeastern United States. However, studies of the genetic diversity and evolution of X. perforans from pepper remain limited. In this study, the whole-genome sequences of 35 X. perforans strains isolated from pepper from four fields and two transplant facilities across southwest Florida between 2019 and 2021 were used to compare genomic divergence, evolution, and variation in type III secreted effectors. Phylogenetic analysis based on core genes revealed that all 35 X. perforans strains formed one genetic cluster with pepper and tomato strains from Alabama and Turkey and were closely related to strains isolated from tomato in Indiana, Mexico, and Louisiana. The in planta population growth of tomato strains isolated from Indiana, Mexico, Louisiana, and Turkey in pepper leaf mesophyll was on par with pepper X. perforans and X. euvesicatoria strains. Molecular clock analysis of the 35 Florida strains dated their emergence to approximately 2017. While strains varied in copper tolerance, all sequenced strains harbored the avrHah1 transcription activation-like effector located on a conjugative plasmid, not previously reported in Florida. Our findings suggest that there is a geographically distributed lineage of X. perforans strains on tomato that has the genetic background to cause disease on pepper. Moreover, this study clarifies potential adaptive variants of X. perforans on pepper that could help forecast the emergence of such strains and enable immediate or preemptive intervention.
Genetic ablation of synaptotagmin‐9 alters tomosyn‐1 function to increase insulin secretion from pancreatic β‐cells improving glucose clearance
Authors: Rahman Md Mostafizur , Pathak Asmita , Schueler Kathryn L. , Alsharif Haifa , Michl Ava , Alexander Justin , Kim Jeong‐A , Bhatnagar Sushant
Published: July 2023

Stimulus‐coupled insulin secretion from the pancreatic islet β‐cells involves the fusion of insulin granules to the plasma membrane (PM) via SNARE complex formation—a cellular process key for maintaining whole‐body glucose homeostasis. Less is known about the role of endogenous inhibitors of SNARE complexes in insulin secretion. We show that an insulin granule protein synaptotagmin‐9 (Syt9) deletion in mice increased glucose clearance and plasma insulin levels without affecting insulin action compared to the control mice. Upon glucose stimulation, increased biphasic and static insulin secretion were observed from ex vivo islets due to Syt9 loss. Syt9 colocalizes and binds with tomosyn‐1 and the PM syntaxin‐1A (Stx1A); Stx1A is required for forming SNARE complexes. Syt9 knockdown reduced tomosyn‐1 protein abundance via proteasomal degradation and binding of tomosyn‐1 to Stx1A. Furthermore, Stx1A‐SNARE complex formation was increased, implicating Syt9‐tomosyn‐1‐Stx1A complex is inhibitory in insulin secretion. Rescuing tomosyn‐1 blocked the Syt9‐knockdown‐mediated increases in insulin secretion. This shows that the inhibitory effects of Syt9 on insulin secretion are mediated by tomosyn‐1. We report a molecular mechanism by which β‐cells modulate their secretory capacity rendering insulin granules nonfusogenic by forming the Syt9‐tomosyn‐1‐Stx1A complex. Altogether, Syt9 loss in β‐cells decreases tomosyn‐1 protein abundance, increasing the formation of Stx1A‐SNARE complexes, insulin secretion, and glucose clearance. These outcomes differ from the previously published work that identified Syt9 has either a positive or no effect of Syt9 on insulin secretion. Future work using β‐cell‐specific deletion of Syt9 mice is key for establishing the role of Syt9 in insulin secretion.
Principles of Justice, Equity, Diversity, and Inclusion in Health Care Distance Simulation Education: Consensus Building via the Nominal Group Technique
Authors: Dowell Sharon , Bajwa Maria , Charnetski Matt , Lababidi Hani , Vora Samreen , Herrera Edgar , Park Yoon Soo , Palaganas Janice C.
Published: July 2023

Purpose

Health care distance simulation (HCDS) replicates professional encounters through an immersive experience overseen by experts and in which technological infrastructure enriches the learning activity. As HCDS has gained traction, so has the movement to provide inclusive and accessible simulation experiences for all participants. However, established guidelines for best practices in HCDS regarding justice, equity, diversity, and inclusion (JEDI) are lacking. This study aimed to generate consensus statements on JEDI principles in synchronous HCDS education using the nominal group technique (NGT).

Method

Professionals with experience in HCDS education were invited to generate, record, discuss, and vote on ideas that they considered best practices for JEDI. This process was followed by a thematic analysis of the NGT discussion to provide a deeper understanding of the final consensus statements. An independent group of HCDS educators individually reviewed and recorded their agreement or disagreement with the consensus statements created by the NGT process.

Results

Eleven independent experts agreed on 6 key practices for JEDI in HCDS. Educators need to (1) be aware of JEDI principles, (2) be able to define and differentiate JEDI, (3) model JEDI in their environment, (4) have expertise and comfort facilitating conversations and debriefing around JEDI issues, (5) be advocates within their organizations to ensure equitable educational experiences, and (6) achieve JEDI without compromising educational objectives. Experts were divided on the approach to technology to ensure equitable learning experiences: some believed that the most basic technology accessible to all learners should be used, and some believed that the technology used should be determined by the competency of the students or faculty.

Conclusions

Structural and institutional barriers in HCDS education persist despite agreement on key JEDI practices. Conclusive research is needed to guide the optimal policy in HCDS toward creating equitable learning experiences while bridging the digital divide.