bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on the Blue Carbon Trading Market System under Blockchain Technology
Authors: Zhao Changping , Sun Juanjuan , Gong Yu , Li Zhi , Zhou Peter
Published: April 2022

To combat global warming, “carbon neutrality” has gradually become a consensus. Some countries have constructed domestic and regional carbon trading markets, and links to global carbon markets are receiving increasing attention. Blue carbon, an important component of nature-based solutions (NbS), has not received the attention it deserves and is still isolated from the carbon trading market. Based on summarizing the existing achievements of blockchain application in carbon trading, this paper analyzes how to make each participating body provide services in the process of blue carbon production, circulation and trading, and designs the architectural diagram of the blue carbon system under peer-to-peer (P2P) transactions based on the theoretical framework of blockchain 3.0. Utilizing the advantages of decentralization, high transparency and non-tamperability of blockchain, we can realize a highly efficient, low-cost and intelligent blue carbon trading management system. Thus, we can make the world pay more attention to the development and utilization of marine resources and increase investment in blue carbon sink projects; and promote the development of blue carbon market, enrich the carbon trading market, and help achieve “emission reduction without reduction in production”.
Reactivity Effects of Inorganic Content in Biomass Gasification: A Review
Authors: Trubetskaya Anna
Published: April 2022

This review article discusses the effects of inorganic content and mechanisms on raw biomass and char during gasification. The impacts of the inherent inorganics and externally added inorganic compounds are summarized based on a literature search from the most recent 40 years. The TGA and larger-scale studies involving K-, Ca-, and Si-related mechanisms are critically reviewed with the aim of understanding the reaction mechanisms and kinetics. Differences between the reaction pathways of inorganic matter, and subsequent effects on the reactivity during gasification, are discussed. The present results illustrate the complexity of ash transformation phenomena, which have a strong impact on the design of gasifiers as well as further operation and process control. The impregnation and mixing of catalytic compounds into raw biomass are emphasized as a potential solution to avoid reactivity-related operational challenges during steam and CO2 gasification. This review clearly identifies a gap in experimental knowledge at the micro and macro levels in the advanced modelling of inorganics transformation with respect to gasification reactivity.
An Energy-Efficient Start-Up Strategy for Large Variable Speed Hydro Pump Turbine Equipped with Doubly Fed Asynchronous Machine
Authors: Singh Rassiah Raja , Baranidharan Manickavel , Subramaniam Umashankar , Bhaskar Mahajan Sagar , Rangarajan Shriram S. , Abdelsalam Hany A. , Collins Edward Randolph , Senjyu Tomonobu
Published: April 2022

The use of a Doubly Fed Asynchronous Machine (DFAM) provides attractive characteristics and offers operational flexibility in many variable speed generation applications, such as in a hydroelectric pumped storage plant. In a variable speed hydroelectric pumped storage plant, the start-up process of DFAM is identical to the conventional singly fed asynchronous machine, wherein a significant amount of energy is wasted. This paper introduces an energy-efficient start-up strategy in DFAM based hydroelectric pump-turbine. The back-to-back voltage source converter connected to the rotor side is amenable for speed control (real power), braking (regenerative/dynamic), and starting the unit. Further, in this starting technique, the stator circuit of the machine is injected with a low voltage DC supply at starting instead of short-circuiting the windings. This DC injection reduces the slip losses and cuts down the magnetizing current requirement. The magnitude of the required DC supply is estimated based on the machine’s reactive power requirement. Also, the switching of stator winding between the short circuit connection, DC injection, and grid supply is carried out using a changeover switch and determined by the speed of the rotor. The proposed starting strategy is investigated with 250 MW DFAM in Matlab/Simulink environment and experimented with a 2.2 kW DFAM prototype. Test results show that the proposed starting method can conserve more than 26.1 percent of electrical energy in the example application compared to the conventional V/f start-up strategy.
Pumped Storage Hydropower for Sustainable and Low-Carbon Electricity Grids in Pacific Rim Economies
Authors: Gilfillan Daniel , Pittock Jamie
Published: April 2022

Because generating electricity significantly contributes to global greenhouse gas emissions, meeting the 2015 Paris Agreement and 2021 Glasgow Climate Pact requires rapidly transitioning to zero or low-emissions electricity grids. Though the installation of renewables-based generators—predominantly wind and solar-based systems—is accelerating worldwide, electrical energy storage systems, such as pumped storage hydropower, are needed to balance their weather-dependent output. The authors of this paper are the first to examine the status and potential for pumped storage hydropower development in 24 Pacific Rim economies (the 21 member economies of the Asia Pacific Economic Cooperation plus Cambodia, Lao PDR, and Myanmar). We show that there is 195 times the pumped storage hydropower potential in the 24 target economies as would be required to support 100% renewables-based electricity grids. Further to the electrical energy storage potential, we show that pumped storage hydropower is a low-cost, low-greenhouse-gas-emitting electrical energy storage technology that can be sited and designed to have minimal negative (or in some cases positive) social impacts (e.g., requirements for re-settlement as well as impacts on farming and livelihood practices) and environmental impacts (e.g., impacts on water quality and biodiversity). Because of the high potential for pumped storage hydropower-based electrical energy storage, only sites with low negative (or positive) social and environmental impacts such as brownfield sites and closed-loop PSH developments (where water is moved back and forth between two reservoirs, thus minimally disturbing natural hydrology) need be developed to support the transition to zero or low-carbon electricity grids. In this way, the advantages of well-designed and -sited pumped storage hydropower can effectively address ongoing conflict around the social and environmental impacts of conventional hydropower developments. Noting the International Hydropower Association advocacy for pumped storage hydropower, we make recommendations for how pumped storage hydropower can sustainably reduce electricity-sector greenhouse gas emissions, including through market reforms to encourage investment and the application of standards to avoid and mitigate environmental and social impacts.
Positive Torque Modulation Method and Key Technology of Conventional Beam Pumping Unit
Authors: Xu Jinchao , Meng Siyuan , Li Wei , Wang Yan
Published: April 2022

The large fluctuation of net torque and the existence of negative torque on the crank output shaft of the beam pumping unit are the decisive factors leading to its low efficiency and high energy consumption. The conventional pumping unit CYJ10-4.2-53HF was selected as the study object on the basis of the fixed shaft secondary balance principle and the positive torque modulation scheme was first proposed according to the following secondary balance principle based on the linkage. The kinematics analysis of the suspension point and the secondary incremental velocity mechanism were carried out using the theory of rigid body plane kinematics. The force analysis of each moving part of the pumping unit was carried out, the net torque expression of the crank output shaft was obtained, and an example calculation was performed. The positive torque beam pumping unit was developed and tested in a field test. The tests showed that the positive torque beam pumping unit was able to fully realize positive torque operation under field well conditions, with a power saving rate of 23.73% and a 14.5% increase in system efficiency, and that the reliability of the pumping unit meets the requirements for field application.
Assessment of Environmental Loads in the Life Cycle of a Retail and Service Building
Authors: Tomporowski Daniel , Kasner Robert , Franus Wojciech , Doerffer Krzysztof
Published: April 2022

In order to achieve the European Union’s climate and energy goals, investments are required, mainly in the areas of energy efficiency, renewable energy sources and infrastructure. Buildings are responsible for almost half of total energy consumption, and nearly 80% of them are energy and ecologically inefficient. The policy of European countries is increasingly more focused on facilities with the highest potential in the areas of energy and matter saving and the possibly circular economy. The aim of the work was to assess the environmental loads occurring in the life cycle of an existing retail and service building. The analysis was performed using the Life Cycle Assessment (LCA) method. By using the IMPACT 2002+ model, it has become possible to assess the impact of the life cycle of the studied facility on human health, environmental quality, climate change and raw material resources. The highest level of negative consequences in the above-mentioned areas was recorded for the life cycle with the disposal in the form of landfill storage. The operational stage was the stage in the life cycle that caused the most harmful impacts on the environment. Therefore, it is necessary to optimize the ecological and energy consumption of resources, for example, by selecting the size and cubature of the facility for its function, maintaining good technical condition, introducing improvements in the usage processes or implementing solutions aimed at reducing media consumption. As a result of the conducted analyses, it can be noticed that in the future, the reduction in energy consumption in the operation of buildings will be of fundamental importance.
A Semantically Data-Driven Classification Framework for Energy Consumption in Buildings
Authors: Popa Angela , Ramallo González Alfonso P. , Jaglan Gaurav , Fensel Anna
Published: April 2022

Encouraged by the European Union, all European countries need to enforce solutions to reduce non-renewable energy consumption in buildings. The reduction of energy (heating, domestic hot water, and appliances consumption) aims for the vision of near-zero energy consumption as a requirement goal for constructing buildings. In this paper, we review the available standards, tools and frameworks on the energy performance of buildings. Additionally, this work investigates if energy performance ratings can be obtained with energy consumption data from IoT devices and if the floor size and energy consumption values are enough to determine a dwellings’ energy performance rating. The essential outcome of this work is a data-driven prediction tool for energy performance labels that can run automatically. The tool is based on the cutting edge kNN classification algorithm and trained on open datasets with actual building data such as those coming from the IoT paradigm. Additionally, it assesses the results of the prediction by analysing its accuracy values. Furthermore, an approach to semantic annotations for energy performance certification data with currently available ontologies is presented. Use cases for an extension of this work are also discussed in the end.
A Survey of Application of Mechanical Specific Energy in Petroleum and Space Drilling
Authors: Khalilidermani Mitra , Knez Dariusz
Published: April 2022

The optimization of drilling operations is an ongoing necessity since the major proportion of the terrestrial hydrocarbon reservoirs has been exhausted. Furthermore, there is a growing tendency among the space exploration agencies to drill the subsurface formations of the remote planets, such as the Moon and Mars. To optimize the drilling efficiency in such complicated conditions, the mechanical specific energy (MSE) must be efficiently reduced. The available MSE models incorporate the different parameters related to the surface rig, drill bit, and the underlying rocks to estimate the MSE values. In this research, the current status of those MSE models is assessed, and their relevant assumptions, limitations, applications, and pros and cons are profoundly argued. From the current scrutiny, it was deduced that the available MSE models require more geomechanical parameters to be included in their formulations. Furthermore, the use of artificial intelligence (AI) techniques was identified as an effective solution to incorporate such geomechanical parameters in the MSE models. Moreover, the establishment of suitable MSE models for off-Earth drilling applications was also revealed to be very urgent and essential. The performed analyses together with the comparative assessments are contributing factors for the modification and establishment of future MSE models.
Greening of the District Heating Systems—Case Study of Local Systems
Authors: Wyrwa Artur , Raczyński Maciej , Kulik Maciej , Oluwapelumi Oluwalana , Mateusiak Laura , Zhang Haoran , Kempka Marek
Published: April 2022

The integration of renewable energy resources into district heating systems is gaining momentum across Europe, as heat producers are expected to work towards the EU Directive of Efficient District Heating and Cooling to achieve carbon neutrality by 2050. This paper studies the techno-economic implications of transforming conventional district heating systems of six locations in Poland, generating 8.5 PJ of heat annually, into sustainable and efficient district heating systems. These new systems consist of flat solar collectors integrated with seasonal pit thermal energy storages and gas heating plants, acting as flexible heat sources, covering residual heat demand and/or increasing the parameters of the working medium in the network. Using the IEA-TIMES software, two scenarios were considered, namely STAT and DYN. The results show that reaching a 20% share of heat production by solar thermal would demand extra construction of seasonal heat storage facilities with a total capacity of 197 TJ, which is approximately 4.5 times bigger than the largest seasonal heat storage located in Vojens, Denmark. The projected increase in the prices of natural gas and CO2 emission allowances accelerates the transformation of systems towards greater use of solar heating plants. In the period 2025–2050 the heat generation costs increase by ca. 65%. The contribution of the CAPEX and OPEX costs components are presented.
Modelling Coal Dust Explosibility of Khyber Pakhtunkhwa Coal Using Random Forest Algorithm
Authors: Khan Amad Ullah , Salman Saad , Muhammad Khan , Habib Mudassar
Published: April 2022

Coal dust explosion constitutes a significant hazard in underground coal mines, coal power plants and other industries utilising coal as fuel. Knowledge of the explosion mechanism and the factors causing coal explosions is essential to investigate for the identification of the controlling factors for preventing coal dust explosions and improving safety conditions. However, the underlying mechanism involved in coal dust explosions is rarely studied under Artificial Intelligence (AI) based modelling. Coal from three different regions of Khyber Pakhtunkhwa, Pakistan, was tested for explosibility in 1.2 L Hartmann apparatus under various particle sizes and dust concentrations. First, a random forest algorithm was used to model the relationship between inputs (coal dust particle size, coal concentration and gross calorific value (GCV)), outputs (maximum pressure (Pmax) and the deflagration index (Kst)). The model reported an R2 value of 0.75 and 0.89 for Pmax and Kst. To further understand the impact of each feature causing explosibility, the random forest AI model was further analysed for sensitivity analysis by SHAP (Shapley Additive exPlanations). The study revealed that the most critical parameter affecting the explosibility of coal dust were particle size > GCV > concentration for Pmax and GCV > Particle size > Concentration for Kst. Mutual interaction SHAP plots of two variables at a time revealed that with <200 gm/L concentration, −73 µm size and a high GCV coal was the most explosive at a high concentration (>400 gm/L), explosibility is relatively lower irrespective of GCV and particle sizes.
Determining the Stability of a Mobile Manipulator for the Transport and Assembly of Arches in the Yielding Arch Support
Authors: Krauze Krzysztof , Mucha Kamil , Wydro Tomasz , Klempka Ryszard , Kutnik Andrzej , Hałas Waldemar , Ruda Piotr
Published: April 2022

The yielding arch support is transported and installed in the face with the use of auxiliary machines. These activities in underground mining cause many problems, which have as yet not been solved. Currently, transport and assembly are carried out manually, using the roadheader and suspended rail, or various types of mounting platforms. The analysis of the structure of the existing solutions resulted in the development of an original structure that met the requirements of Polish mines. Developed jointly by FAMA Sp. z o.o. and the AGH University of Science and Technology in Krakow, Poland, the mining modular transport and assembly unit (MZT-M) will enable the transport and assembly of support arches in the mining face. Additionally, it can also be used to reload works, which is related to the work ergonomics in underground coal mining, which is the main energy resource in Poland. The most important problem to be solved in the case of this manipulator, due to the limited space in the excavation, is how to ensure its stability during various phases of its operation. Therefore, analyses were carried out to find a solution, which resulted in determining specific conditions and design requirements related to the operation of this manipulator.
Lactobacillus fermentum Stimulates Intestinal Secretion of Immunoglobulin A in an Individual-Specific Manner
Authors: Mei Liya , Chen Ying , Wang Jialiang , Lu Jian , Zhao Jianxin , Zhang Hao , Wang Gang , Chen Wei
Published: April 2022

Immunoglobulin A (IgA), as the most secreted immunoglobulin in the intestine, plays an irreplaceable role in mucosal immunity regulation. Previous studies have indicated that Lactobacillus showed strain specificity in stimulating the secretion of IgA through intestinal mucosal lymphocytes. The reason for this phenomenon is not clear. The current studies have been aimed at exploring the effect of a strain on the secretion of IgA in the host’s intestine, but the mechanism behind it has not been seriously studied. Based on this, we selected five strains of Lactobacillus fermentum isolated from different individuals to determine whether there are intraspecific differences in stimulating the secretion of IgA from the intestinal mucosa. It was found that IgA concentrations in different intestinal segments and faeces induced by L. fermentum were different. 12-1 and X6L1 strains increased the secretion of IgA by the intestine significantly. In addition, different strains of L. fermentum were also proven to have different effects on the host gut microbiota but no significant effects on IgA-coated microbiota. Besides, it was speculated that different strains of L. fermentum may act on different pathways to stimulate IgA in a non-inflammatory manner. By explaining the differences of IgA secretion in the host’s intestine tract stimulated by different strains of L. fermentum, it is expected to provide a theoretical basis for the stimulation of intestinal secretion of IgA by Lactobacillus and a new direction for exploring the relationship between Lactobacillus and human immunity.
Role of Bioactive Compounds in Obesity: Metabolic Mechanism Focused on Inflammation
Authors: Ramírez-Moreno Esther , Arias-Rico José , Jiménez-Sánchez Reyna Cristina , Estrada-Luna Diego , Jiménez-Osorio Angélica Saraí , Zafra-Rojas Quinatzin Yadira , Ariza-Ortega José Alberto , Flores-Chávez Olga Rocío , Morales-Castillejos Lizbeth , Sandoval-Gallegos Eli Mireya
Published: April 2022

Obesity is a disease characterized by an inflammatory process in the adipose tissue due to diverse infiltrated immune cells, an increased secretion of proinflammatory molecules, and a decreased secretion of anti-inflammatory molecules. On the other hand, obesity increases the risk of several diseases, such as cardiovascular diseases, diabetes, and cancer. Their treatment is based on nutritional and pharmacological strategies. However, natural products are currently implemented as complementary and alternative medicine (CAM). Polyphenols and fiber are naturally compounds with potential action to reduce inflammation through several pathways and play an important role in the prevention and treatment of obesity, as well as in other non-communicable diseases. Hence, this review focuses on the recent evidence of the molecular mechanisms of polyphenols and dietary fiber, from Scopus, Science Direct, and PubMed, among others, by using key words and based on recent in vitro and in vivo studies.
Sensory Analysis for Cow Milk Product Development Using High Pressure Processing (HPP) in the Dairy Industry
Authors: Lim Shu Huey , Chin Nyuk Ling , Sulaiman Alifdalino , Tay Cheow Hwang , Wong Tak Hiong
Published: April 2022

High pressure processing (HPP) can be applied as an alternative thermal treatment of milk to maintain its natural and original sensory quality. Milk was processed at 600 MPa for 10 min or given thermal treatment at 125 °C for 4 s. Sensory evaluation of treated milk samples was conducted using the triangle and the acceptance and preference tests. The triangle test was used as a discriminative test to check whether there was a noticeable difference between both treated milk samples. The acceptance and preference test determined attributes of milk including colour, milkiness, creaminess, mouthfeel, and aftertaste based on the 5-point just-about-right (JAR) scale. In the triangle test, 89.5% of panellists were able to identify the odd sample and differentiate milk processed using high pressure from heat treatment. For the acceptance and preference test, 61% of panellists gave higher overall preference for the high pressure processed milk over heat-treated milk. The JAR evaluation showed no significant differences (p > 0.05) in all evaluated milk attributes which included milkiness, creaminess, mouthfeel, and aftertaste, with the exception of colour. Overall, high pressure processed milk scored better in terms of organoleptic properties as the penalty value for most attributes including colour, milkiness, mouthfeel, and aftertaste were lower than the penalty of heat-treated milk, except for creaminess. Therefore, to improve the acceptance and preference of high pressure processed milk, future development needs to focus on increasing creaminess of high pressure processed milk.
Assessment of Ultrasonic Stress on Survival and β-Glucosidase Activity of Encapsulated Lactiplantibacillus plantarum BCRC 10357 in Fermentation of Black Soymilk
Authors: Tseng Hung-Chih , Yang Chun-Yao
Published: April 2022

The enhanced β-glucosidase activity of encapsulated Lactiplantibacillus plantarum BCRC 10357 within calcium alginate capsules was investigated by ultrasonic stimulation to induce the stress response of the bacteria for the biotransformation of isoflavones in black soymilk. The effects of various ultrasound durations, sodium alginate concentrations (% ALG), and cell suspensions on the β-glucosidase activity of encapsulated bacteria were explored. The β-glucosidase activity of encapsulated L. plantarum BCRC 10357 with ultrasonic stimulation (40 kHz/300 W) was greater than that without ultrasound. With 20 min of ultrasonic treatment, the β-glucosidase activity of encapsulated L. plantarum BCRC 10357 from 2% ALG/0.85% NaCl cell suspension was 11.47 U/mL at 12 h, then increased to 27.43 U/mL at 36 h and to 26.25 U/mL at 48 h in black soymilk at 37 °C, showing the high adaptation of encapsulated L. plantarum BCRC 10357 encountering ultrasonic stress to release high β-glucosidase until 48 h, at which point the ratio of isoflavone aglycones (daidzein and genistein) in total isoflavones (daidzin, genistin, daidzein, and genistein) was 98.65%, reflecting the effective biotransformation of isoflavone glycosides into aglycones by β-glucosidase. In this study, the survivability and β-glucosidase activity of encapsulated L. plantarum BCRC 10357 were enhanced under ultrasonic stimulation, and were favorably used in the fermentation of black soymilk.
Antibacterial Effects of Bacteriocin PLNC8 against Helicobacter pylori and Its Potential Mechanism of Action
Authors: Liang Ying , Yan Jiaqian , Chen Ziqi , Gu Qing , Li Ping
Published: April 2022

Helicobacter pylori (H. pylori) is a bacterium that can cause a variety of gastric diseases. Most bacteriocins have gained popularity due to their non-toxic effects on cells and antibacterial effects against a wide range of pathogenic bacteria. In this study, the chemical synthesis of the bipeptide bacteriocin PLNC8 was used to investigate its possible action mechanism against H. pylori ZJC03 in vitro. Results showed that PLNC8 had significant anti-H. pylori ZJC03 potential, which resulted in a significant reduction in urease activity and a minimum inhibitory concentration (MIC) of 80 μM. PLNC8 inhibited the growth of H. pylori ZJC03, disrupting its structure as observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). In addition, PLNC8 decreased the ATP level and hydrogen peroxide sensitivity of H. pylori ZJC03. In conclusion, PLNC8 disrupts the ability of H. pylori ZJC03 to alter the host environment, providing a new avenue for the prevention and control of H. pylori infection, providing a theoretical foundation for further elucidation of its regulatory mechanism.
Quality Characteristics of Meat Analogs through the Incorporation of Textured Vegetable Protein: A Systematic Review
Authors: Bakhsh Allah , Lee Eun-Yeong , Ncho Chris Major , Kim Chan-Jin , Son Yu-Min , Hwang Young-Hwa , Joo Seon-Tea
Published: April 2022

Meat analogs produced through extruded products, such as texture vegetable protein (TVP) with the addition of various plant-based ingredients are considered the products that have great potential for replacing real meat. This systematic review was conducted to summarize the evidence of the incorporation of TVP on the quality characteristics of meat analogs. Extensive literature exploration was conducted up to March 2022 for retrieving studies on the current topic in both PubMed and Scopus databases. A total of 28 articles published from 2001 to 2022 were included in the data set based on specific inclusion criteria. It appears that soy protein is by far the most used extender in meat analogs due to its low cost, availability, and several beneficial health aspects. In addition, the studies included in this review were mainly conducted in countries, such as Korea, the USA, and China. Regarding quality characteristics, textural parameters were the most assessed in the studies followed by physicochemical properties, and sensory and taste attributes. Other aspects, such as the development of TVP, the difference in quality characteristics of texturized proteins, and the usage of binding agents in various meat analogs formulations are also highlighted in detail.
Determination of Market, Field Samples, and Dietary Risk Assessment of Chlorfenapyr and Tralopyril in 16 Crops
Authors: Li Hong , Sun Fengshou , Hu Shuai , Sun Qi , Zou Nan , Li Beixing , Mu Wei , Lin Jin
Published: April 2022

The frequent and massive use of chlorfenapyr has led to pesticide residues in crops, threatening food safety and human health. However, there is limited research on the detection of tralopyril, which is the major metabolite of chlorfenapyr with high toxicity. This study aimed to develop a novel, sensitive, and highly efficient method for the determination of chlorfenapyr and tralopyril residues in 16 crops. The optimized purification procedure provided satisfactory recovery of 76.6–110%, with relative standard deviations of 1.3–11.1%. The quantification values of pesticides in crop matrixes were all 0.01 μg kg−1. The optimal method was adopted to determine the chlorfenapyr and tralopyril residues in field trials in 12 regions in China and monitor their residues in 16 agricultural products. The results of the dissipation and terminal residue experiments show that the final residue of chlorfenapyr was less than MRL (maximum residue limit) and no tralopyril was detected in the field samples. Moreover, the qualification proportion of these residues in market samples were up to 99.5%. The RQ (risk quotient) values of chlorfenapyr and chlorfenapyr with consideration of tralopyril were both apparently lower than an RQ of 100%, indicating an acceptable level. This research provides a thorough long-term dietary risk evaluation on chlorfenapyr and tralopyril and would provide reference for their scientific and safe utilization.
Boundary Value Problem for Fractional q-Difference Equations with Integral Conditions in Banach Spaces
Authors: Allouch Nadia , Graef John R. , Hamani Samira
Published: April 2022

The authors investigate the existence of solutions to a class of boundary value problems for fractional q-difference equations in a Banach space that involves a q-derivative of the Caputo type and nonlinear integral boundary conditions. Their result is based on Mönch’s fixed point theorem and the technique of measures of noncompactness. This approach has proved to be an interesting and useful approach to studying such problems. Some basic concepts from the fractional q-calculus are introduced, including q-derivatives and q-integrals. An example of the main result is included as well as some suggestions for future research.
Quantization for Infinite Affine Transformations
Authors: Çömez Doğan , Roychowdhury Mrinal Kanti
Published: April 2022

Quantization for a probability distribution refers to the idea of estimating a given probability by a discrete probability supported by a finite set. In this article, we consider a probability distribution generated by an infinite system of affine transformations {Sij} on R2 with associated probabilities {pij} such that pij>0 for all i,j∈N and ∑i,j=1∞pij=1. For such a probability measure P, the optimal sets of n-means and the nth quantization error are calculated for every natural number n. It is shown that the distribution of such a probability measure is the same as that of the direct product of the Cantor distribution. In addition, it is proved that the quantization dimension D(P) exists and is finite; whereas, the D(P)-dimensional quantization coefficient does not exist, and the D(P)-dimensional lower and the upper quantization coefficients lie in the closed interval [112,54].
Understanding High-Energy (UV and X-ray) Emission from AGB Stars—Episodic Accretion in Binary Systems
Authors: Sahai Raghvendra , Sanz-Forcada Jorge , Guerrero Martin , Ortiz Roberto , Contreras Carmen Sanchez
Published: April 2022

X-ray surveys of UV-emitting AGB stars show that ∼40% of objects with FUV emission and GALEX FUV/NUV flux ratio Rfuv/nuv ∼> 0.2 (fuvAGB stars) have variable X-ray emission characterized by very high temperatures (Tx∼35–160 MK) and luminosities (Lx∼0.002–0.2 L⊙), indicating the presence of accretion associated with a close binary companion. However, the UV-emitting AGB star population is dominated by objects with Rfuv/nuv ≲ 0.06 (nuvAGB stars), and we do not know whether the UV emission from these is intrinsic to the AGB star or extrinsic (i.e., due to binarity). In order to help distinguish between intrinsic and extrinsic models of the puzzling high-energy emission of cool AGB stars, we report results from two studies—(i) XMM-Newton X-observations of two nuvAGB stars, and (ii) simple chromosphere modeling. In study (i), we detect the one which has the lower FUV/NUV ratio, with a total Lx = 0.00027 L⊙, and a spectrum best fitted with a dominant component at Tx∼10 MK, most likely coronal emission from a main-sequence companion. Therefore, a significant fraction of nuvAGB stars may also be binaries with active, but weak accretion. Study (ii) shows that chromospheres with temperatures of ∼10,000 K can produce Rfuv/nuv≲0.06; higher ratios require hotter gas, implying active accretion.
Progesterone Gel and Placebo Prolonged Pregnancy More Effectively Than Intravenous Tocolysis Alone in Women with Preterm Labor
Authors: Vladic Stjernholm Ylva , Vladic Tomislav , Marchini Giovanna
Published: April 2022

The aim of this trial was to evaluate the effect of progesterone gel compared to placebo in prolonging pregnancy among women with preterm labor. Methods: A randomized controlled trial in Sweden in 2009–18. Women with early preterm labor were randomized to daily doses of progesterone gel 90 mg (n = 28) or placebo (n = 30) after standard intravenous tocolytics. Women with intravenous tocolytics alone (n = 29) served as controls. Results: The median latency to delivery was 68 (range 28–88) days with progesterone and 72 (range 9–90) days with placebo (p = 0.84), compared to 1 (range 1–2) day in the control group (progesterone and placebo vs. control p < 0.001). The rate of preterm birth before 34 weeks was 32% after progesterone and 37 % after placebo (p = 0.32) compared to 100 % in the control group (p < 0.001, respectively). The composite neonatal morbidity (p = 0.65) and neonatal intensive care unit admission (p = 0.12) were comparable between the progesterone and placebo groups and lower in these groups compared with neonates in the control group (p < 0.001, respectively). Conclusions: Progesterone gel and placebo were equally effective in prolonging pregnancy among women with early preterm labor, and both treatments were more effective than standard intravenous tocolysis alone. We hypothesize that the acidic placebo gel reinforced the biochemical barrier at the uterine cervix, which counteracts ascending pathogen invasion and subsequent inflammation, and thereby prevented preterm labor.
Burden of Mental Illness among Primary HIV Discharges: A Retrospective Analysis of Inpatient Data
Authors: Avina Robert M. , Banta Jim E. , Mataya Ronald , Becerra Benjamin J. , Becerra Monideepa B.
Published: April 2022

Background: Empirical evidence demonstrates the substantial burden of mental illness among people living with HIV and AIDS (PLWHA). Current literature also notes the co-morbidity of these two illnesses and its impact on quality of life and mortality. However, little evidence exists on patient outcomes, such as hospital length of stay or post-discharge status. Methods: A retrospective analysis of National Inpatient Sample data was conducted. The study population was defined as discharges having a primary diagnosis of HIV based on International Classification of Disease, 10th Revision, Clinical Modification (ICD-10-CM) codes in primary diagnosis field. Clinical Classification Software (CCS) codes are used to identify comorbid mental illness. Length of stay was defined as number of days between hospital admission and discharge. Disposition (or post-discharge status) was defined as routine versus not routine. Patient and hospital characteristics were used as control variables. All regression analyses were survey-weighted and adjusted for control variables. Results: The weighted population size (N) for this study was 26,055 (n = 5211). Among primary HIV discharges, presence of any mental illness as a secondary discharge was associated with 12% higher LOS, when compared to a lack of such comorbidity (incidence rate ratio [IRR] = 1.12, 95% confidence interval [CI] = 1.05, 1.22, p < 0.01). Likewise, among primary HIV discharges, those with mental illness had a 21% lower routine disposition, when compared to those without any mental illness (OR = 0.79, 95% CI = 0.68, 0.91, p < 0.001). Conclusion: Our results highlight the need for improved mental health screening and coordinated care to reduce the burden of mental illness among HIV discharges.
Spatial Variability and Clustering of Quality of Life at Local Level: A Geographical Analysis in Athens, Greece
Authors: Faka Antigoni , Kalogeropoulos Kleomenis , Maloutas Thomas , Chalkias Christos
Published: April 2022

This paper presents a geographical analysis to evaluate urban quality of life in Athens, Greece, and investigate spatial heterogeneity and potential clustering. The urban environment was examined using composite criteria related to natural, built and socioeconomic environment, housing conditions, public services and infrastructures, and cultural and recreational facilities. Each criterion constructed from a set of mappable sub-criteria/variables. Weighted cartographic overlay was implemented to assess the overall urban quality of life of each spatial unit, based on the importance the residents of the area attributed to each criterion. High levels of quality of life were revealed in the eastern neighborhoods of the municipality, whereas low levels were noticed mainly in the western neighborhoods. The results of the study were validated using the perceived quality of life of the study area’s residents, resulting in substantial agreement. Finally, after spatial autocorrelation analysis, significant clustering of urban quality of life in Athens was revealed. The quality-of-life assessment and mapping at a local scale are efficient tools, contributing to better decision making and policy making.
Evidence That β1-Integrin Is Required for the Anti-Viability and Anti-Proliferative Effect of Resveratrol in CRC Cells
Authors: Brockmueller Aranka , Shayan Parviz , Shakibaei Mehdi
Published: April 2022

The β1-integrin receptor is broadly expressed on tumor and other cells in the tumor microenvironment (TME), and is an unfavorable prognostic factor for cancers. Nature-derived resveratrol has preventive and apoptotic effects on tumors, but whether resveratrol can exert its suppressive actions on TME-induced tumorigenesis through β1-integrin on the surface of CRC cells is still unknown. HCT116 or SW480 cells were exposed to inhibitory antibodies against β1-integrin, bacitracin (selective β1-integrin inhibitor), integrin-binding RGD (Arg-Gly-Asp) peptide, and/or resveratrol. We evaluated the anti-tumor actions and signaling impacts of resveratrol in colorectal cancer (CRC)-TME. We found that resveratrol completely altered the β1-integrin distribution pattern and expression on the surface of CRC cells in TME. Moreover, resveratrol down-regulated CRC cell proliferation, colony formation, viability, and up-regulated apoptosis in a concentration-dependent way. These actions of resveratrol were antagonized mainly by inhibitory antibodies against β1-integrin but not β5-integrin, and by an integrin-binding RGD peptide but not by RGE peptide, and by bacitracin in TME. Similarly, resveratrol-blocked TME-induced p65-NF-kB and its promoted gene markers linked to proliferation (cyclin D1), invasion (focal adhesion kinase, FAK), or apoptosis (caspase-3), were largely abrogated by anti-β1-integrin or RGD peptide, suggesting that β1-integrin is a potential transmission pathway for resveratrol/integrin down-stream signaling in CRC cells. The current results highlight, for the first time, the important gateway role of β1-integrins as signal carriers for resveratrol on the surfaces of HCT116 and SW480 cells, and their functional cooperation for the modulatory effects of resveratrol on TME-promoted tumorigenesis.
Receptor-Mediated AKT/PI3K Signalling and Behavioural Alterations in Zebrafish Larvae Reveal Association between Schizophrenia and Opioid Use Disorder
Authors: Thiagarajan Siroshini K. , Mok Siew Ying , Ogawa Satoshi , Parhar Ishwar S. , Tang Pek Yee
Published: April 2022

The link between substance abuse and the development of schizophrenia remains elusive. In this study, we assessed the molecular and behavioural alterations associated with schizophrenia, opioid addiction, and opioid withdrawal using zebrafish as a biological model. Larvae of 2 days post fertilization (dpf) were exposed to domperidone (DMP), a dopamine-D2 dopamine D2 receptor antagonist, and morphine for 3 days and 10 days, respectively. MK801, an N-methyl-D-aspartate (NMDA) receptor antagonist, served as a positive control to mimic schizophrenia-like behaviour. The withdrawal syndrome was assessed 5 days after the termination of morphine treatment. The expressions of schizophrenia susceptibility genes, i.e., pi3k, akt1, slc6a4, creb1 and adamts2, in brains were quantified, and the levels of whole-body cyclic adenosine monophosphate (cAMP), serotonin and cortisol were measured. The aggressiveness of larvae was observed using the mirror biting test. After the short-term treatment with DMP and morphine, all studied genes were not differentially expressed. As for the long-term exposure, akt1 was downregulated by DMP and morphine. Downregulation of pi3k and slc6a4 was observed in the morphine-treated larvae, whereas creb1 and adamts2 were upregulated by DMP. The levels of cAMP and cortisol were elevated after 3 days, whereas significant increases were observed in all of the biochemical tests after 10 days. Compared to controls, increased aggression was observed in the DMP-, but not morphine-, treated group. These two groups showed reduction in aggressiveness when drug exposure was prolonged. Both the short- and long-term morphine withdrawal groups showed downregulation in all genes examined except creb1, suggesting dysregulated reward circuitry function. These results suggest that biochemical and behavioural alterations in schizophrenia-like symptoms and opioid dependence could be controlled by common mechanisms.
Optical and Material Characteristics of MoS2/Cu2O Sensor for Detection of Lung Cancer Cell Types in Hydroplegia
Authors: Mukundan Arvind , Feng Shih-Wei , Weng Yu-Hsin , Tsao Yu-Ming , Artemkina Sofya B. , Fedorov Vladimir E. , Lin Yen-Sheng , Huang Yu-Cheng , Wang Hsiang-Chen
Published: April 2022

In this study, n-type MoS2 monolayer flakes are grown through chemical vapor deposition (CVD), and a p-type Cu2O thin film is grown via electrochemical deposition. The crystal structure of the grown MoS2 flakes is analyzed through transmission electron microscopy. The monolayer structure of the MoS2 flakes is verified with Raman spectroscopy, multiphoton excitation microscopy, atomic force microscopy, and photoluminescence (PL) measurements. After the preliminary processing of the grown MoS2 flakes, the sample is then transferred onto a Cu2O thin film to complete a p-n heterogeneous structure. Data are confirmed via scanning electron microscopy, SHG, and Raman mapping measurements. The luminous energy gap between the two materials is examined through PL measurements. Results reveal that the thickness of the single-layer MoS2 film is 0.7 nm. PL mapping shows a micro signal generated at the 627 nm wavelength, which belongs to the B2 excitons of MoS2 and tends to increase gradually when it approaches 670 nm. Finally, the biosensor is used to detect lung cancer cell types in hydroplegia significantly reducing the current busy procedures and longer waiting time for detection. The results suggest that the fabricated sensor is highly sensitive to the change in the photocurrent with the number of each cell, the linear regression of the three cell types is as high as 99%. By measuring the slope of the photocurrent, we can identify the type of cells and the number of cells.
Improved 3D Printing and Cell Biology Characterization of Inorganic-Filler Containing Alginate-Based Composites for Bone Regeneration: Particle Shape and Effective Surface Area Are the Dominant Factors for Printing Performance
Authors: Bednarzig Vera , Schrüfer Stefan , Schneider Tom C. , Schubert Dirk W. , Detsch Rainer , Boccaccini Aldo R.
Published: April 2022

The use of organic–inorganic 3D printed composites with enhanced properties in biomedical applications continues to increase. The present study focuses on the development of 3D printed alginate-based composites incorporating inorganic fillers with different shapes (angular and round), for bone regeneration. Reactive fillers (bioactive glass 13–93 and hydroxyapatite) and non-reactive fillers (inert soda–lime glass) were investigated. Rheological studies and the characterization of various extrusion-based parameters, including material throughput, printability, shape fidelity and filament fusion, were carried out to identify the parameters dominating the printing process. It was shown that the effective surface area of the filler particle has the highest impact on the printing behavior, while the filler reactivity presents a side aspect. Composites with angular particle morphologies showed the same high resolution during the printing process, almost independent from their reactivity, while composites with comparable amounts of round filler particles lacked stackability after printing. Further, it could be shown that a higher effective surface area of the particles can circumvent the need for a higher filler content for obtaining convincing printing results. In addition, it was proven that, by changing the particle shape, the critical filler content for the obtained adequate printability can be altered. Preliminary in vitro biocompatibility investigations were carried out with the bioactive glass containing ink. The 3D printed ink, forming an interconnected porous scaffold, was analyzed regarding its biocompatibility in direct or indirect contact with the pre-osteoblast cell line MC3T3-E1. Both kinds of cell tests showed increased viability and a high rate of proliferation, with complete coverage of the 3D scaffolds’ surface already after 7 d post cell-seeding.
Development of an Automatic Pipeline for Participation in the CELPP Challenge
Authors: Miñarro-Lleonar Marina , Ruiz-Carmona Sergio , Alvarez-Garcia Daniel , Schmidtke Peter , Barril Xavier
Published: April 2022

The prediction of how a ligand binds to its target is an essential step for Structure-Based Drug Design (SBDD) methods. Molecular docking is a standard tool to predict the binding mode of a ligand to its macromolecular receptor and to quantify their mutual complementarity, with multiple applications in drug design. However, docking programs do not always find correct solutions, either because they are not sampled or due to inaccuracies in the scoring functions. Quantifying the docking performance in real scenarios is essential to understanding their limitations, managing expectations and guiding future developments. Here, we present a fully automated pipeline for pose prediction validated by participating in the Continuous Evaluation of Ligand Pose Prediction (CELPP) Challenge. Acknowledging the intrinsic limitations of the docking method, we devised a strategy to automatically mine and exploit pre-existing data, defining—whenever possible—empirical restraints to guide the docking process. We prove that the pipeline is able to generate predictions for most of the proposed targets as well as obtain poses with low RMSD values when compared to the crystal structure. All things considered, our pipeline highlights some major challenges in the automatic prediction of protein–ligand complexes, which will be addressed in future versions of the pipeline.