bluuebunny/crossref_metadata_2025 huggingface dataset:
Appreciative Leadership, Workplace Belongingness, and Affective Commitment of Nurses: The Mediating Role of Job Crafting
Authors: Saleh Manal Saleh Moustafa , Abd-Elhamid Zaineb Naiem , Afit Aldhafeeri Nouf , Ghaleb Dailah Hamad , Alenezi Atallah , Zoromba Mohamed , Elsaid Elsabahy Hanan
Published: July 2024

Aim. This study aimed to investigate the appreciation leadership, workplace belongingness, and affective commitment among nurses, with a specific focus on the mediating role of job crafting. Background. Leadership, particularly in healthcare care, significantly influences employee experiences and outcomes. Appreciative leadership fosters a positive work environment, valuing and motivating employees. However, its impact on workplace belongingness and affective commitment among nurses requires further exploration. Job crafting, a mechanism in which employees shape their roles to align with their preferences, strengths, and values, can serve as a mediator in the relationship between appreciative leadership and outcomes, such as workplace belongingness and affective commitment. Subjects and Methods. A cross‐sectional descriptive study was conducted in nurses from two hospitals (Prince Mohammed bin Abdulaziz and Shaqra General Hospital) in Riyadh City, Saudi Arabia. Four standardized scales were used to assess appreciation for leadership, sense of belonging, affective commitment among nurses, and job crafting; 381 nurses were surveyed. AMOS structural equation modeling (SEM) was used to examine the hypothetical model of the study. Results. APL significantly affects job‐crafting behaviors, belonging, and affective commitment among nurses. Furthermore, job‐crafting behaviors significantly affect belonging among nurses and commitment. Conclusions. This indicates that when nurses perceive their leadership positively, their job‐crafting behaviors increase, which in turn enhances their sense of belonging at work. Furthermore, these findings indicate that positive leadership perceptions directly improve nurses’ commitment to their jobs. This study recommended that educational programs can upgrade leadership styles and change practice levels. Implications for Nursing Management. Nursing managers should focus on cultivating appreciative leadership behaviors, such as providing regular feedback, recognizing achievements, and fostering a supportive work culture. Organizations can encourage the creation of jobs among nurses by offering opportunities for autonomy, skill development, and flexibility in job roles.
Exploring the Dynamics of Fractional q‐Integro‐Differential Equations with Infinite Time Delays: A Study in Mathematical Analysis
Authors: Alsallami Shami A. M. , Raslan K. R. , Khalil E. M. , Abdel-Khalek S. , Abd-Elall Ibrahim Amira , Ali Khalid K.
Published: July 2024

A novel equation that combines fractional calculus and integral operations is investigated in this study. The unique properties of the equation and its potential applications to various real‐world phenomena have not been previously explored. The existence and uniqueness of a solution to this equation are the primary objectives of this research. To achieve this, the Schauder fixed point theorem and Banach’s fixed point theorem are utilized. The role of these theorems is crucial in demonstrating the existence and uniqueness of the solution. In addition to theoretical analysis, numerical methods are developed, employing the Caputo fractional derivative and the Riemann fractional q integral. By combining the finite difference approach with the trapezoidal method, the equation is transformed into a system of algebraic equations that can be efficiently solved. This numerical approach allows the acquisition of a reliable numerical solution to the problem at hand. Practical examples are presented to underscore the significance of the findings and to showcase the diverse applications of this equation. These examples serve as compelling evidence for the profound impact of this study on real‐world phenomena. Through rigorous mathematical reasoning and innovative numerical techniques, the mysteries surrounding this intriguing equation are uncovered. Consequently, this exploration not only contributes to a deeper understanding of fractional calculus but also sheds light on its practical implications in a variety of real‐world scenarios.
Response Characteristics of Tunnels with Curvilinear Axes under Obliquely Incident Lateral SV Seismic Waves
Authors: Sun Yongchao , Wang Meijing , Ai Chengjie , Fan Chuanwei , Zhao Huiling
Published: July 2024

Influenced by topography, geological conditions, and environmental factors, the alignment design of long‐distance tunnels frequently incorporates curved tunnel segments. When subjected to obliquely incident transverse SV seismic waves, curved tunnels exhibit more complex patterns of stress and deformation, which could become pivotal in governing the seismic safety of the tunnel compared to their straight counterparts. This paper presents a numerical simulation on the response characteristics of curved tunnels subjected to obliquely incident lateral seismic SV waves. Curved tunnels exhibit more complex deformation under oblique SV wave incidence compared to vertical incidence, with notable increases in axial tensile deformation and shear deformation, both axially and in‐plane, as the angle of incidence rises. While vertical incidence primarily induces lateral bending, oblique incidence results in bending influenced by both wave direction and incidence angle, necessitating advanced seismic design considerations for tunnel integrity. The bending moment distribution in curved tunnels under oblique incidence displays nonuniformity and localized concentration. The findings underscore the importance of considering the oblique incident angle in the design and analysis of curved tunnels for seismic safety.
Aspects of Dual Simulation for Modified Thermal Flux Advances in Non‐Newtonian Reiner–Philippoff Fluid Flow past a Shrinking Plate Embedded in a Porous Medium
Authors: Mohamad Nurhana , Ishak Anuar , Khan Umair , Rawat Sawan Kumar , Siddiqui Md Irfanul Haque
Published: July 2024

This study investigates the effects of non‐Newtonian Reiner–Philippoff fluids on porous media, particularly in the context of steady radiative mixed convection flow and heat transfer near a shrinking plate surface in the presence of magnetohydrodynamics (MHD). The mathematical model is constructed using PDEs and transformed into ODEs via similarity transformations, with numerical solutions obtained using MATLAB’s bvp4c function. The results demonstrate that boundary layer separation occurs slowest for dilatant fluids and fastest for pseudoplastic fluids, with Newtonian fluids exhibiting moderate separation rates. Thermal radiation and media porosity parameters are found to reduce heat transfer by approximately 0.95% and 0.02%, respectively, while accelerating boundary layer separation. Conversely, magnetic effects and suction parameters increase heat transfer by about 0.08% and 4.25%, respectively, enhancing both fluid velocity and temperature. The mixed convection parameter indicates the possibility of dual solutions, with the opposing flow favoring this phenomenon more than the assisting flow. The time‐based stability analysis reveals that the first solution is stable, whereas the second solution is unstable. These findings provide significant insights into the behavior and control of Reiner–Philippoff fluids in practical applications involving porous media and magnetic fields.
Hydatid of Morgagni in Young Female: A Rare Case of Acute Abdomen—Case Report and Literature Review
Authors: Bollettini Taisia , Molinaro Francesco , Benigna Alice , Ciardini Enrico , Centini Gabriele , Calomino Natale , Fusi Giulia , Mario Messina , Angotti Rossella
Published: July 2024

Torsion of the hydatid of Morgagni is a rare condition which can cause acute abdominal pain in young female. We present a case report of a 13‐year‐old girl with acute abdominal pain and treated for torsion of the hydatid of Morgagni. Less than 20 cases of female younger than 18 years old with this condition are been described in the literature. Through our systematic review performed following the PRISMA statement, we want to emphasize the difficulty in diagnosis and the importance of considering this pathology in the differential diagnosis of acute abdomen in females with the aim of obtaining a timely surgical treatment to preserve fertility in these patients.
Inner‐Side‐Protected Cladding with Meter Scale for High‐Temperature Oxidation Resistance via the Swaging–Drawing Process
Authors: Jo Jeong-Hye , Choi Ji-Hyeok , Kim Jong Woo , Kim Dong-Joo , Yoon Young Soo
Published: July 2024

Nuclear accidents, such as the Fukushima nuclear accident, have highlighted the necessity for accident‐tolerant fuel (ATF) cladding. Previous studies focused on coating the outside of Zr alloy currently used in nuclear reactors with an oxidation‐resistant material in a vacuum environment. This limits the coating to the inside of the cladding and does not tend to achieve a uniform coating on meter scale cladding. In this study, a room temperature and non‐vacuum‐based swaging–drawing process was demonstrated as an alternative cladding manufacturing process. It enables both the inner and outer sides of the 2‐m‐long Zr alloy cladding to be uniformly covered with a 100‐μm‐thick corrosion‐resistant material (316‐L stainless steel; SS316L), thereby minimizing its high‐temperature oxidation and avoiding failures. After the swaging–drawing process, there was a gap of less than 1 µm between outer SS316L and Zr alloy and a gap of about 12 µm between inner Zr alloy and SS316L. The high‐temperature oxidation properties of the resulting triplex Gachon ATF cladding tube (G‐tube) were evaluated up to 1,200°C in an atmospheric environment. Following heat treatment at 1,200°C, the control cladding completely oxidized and ruptured, potentially causing leakage of radioactive material during application. In contrast, only 15% of the G‐tube cladding manufactured by the swaging–drawing process was oxidized despite a gap, and the Zr alloy of the G‐tube changed phase from α‐Zr to α‐Zr (O) and prior β‐Zr. The cladding microstructure, oxide layer, and oxidation mechanism were analyzed through microscopy, X‐ray diffraction, and thermogravimetric analysis. As a result, it was confirmed that SS316L completely prevented oxygen diffusion into the bulk Zr alloy. In addition, there was no elemental diffusion between SS316L and the Zr alloy. These results demonstrate the feasibility of using room temperature, nonvacuum environment‐based swaging–drawing process to fabricate structurally stable ATF cladding at extremely high temperatures.
Dual‐Sliding‐Mode‐Observer‐Based IPMSM Sensorless Control Technique
Authors: Xu Sang , Shen Anwen , Zhang Mingzhen , Tang Qipeng , Luo Xin , Xu Jinbang
Published: July 2024

Back electromotive force (EMF)‐based sliding mode observer (SMO) is increasingly employed for interior permanent magnet synchronous machine (IPMSM) sensorless drives due to its high robustness to external disturbance and low sensitivity to system parameter variations. However, its control performance is severely weakened by the inherent chattering and speed iteration operation. In order to effectively resolve these problems, a strategy to design a dual‐SMO is proposed in this paper. With the proposed strategy, the combination of the stator‐voltage transformation matrix (SVTM) and the low‐pass filter is developed to obtain the rotor position information, which greatly alleviates the chattering without any deviations. Meanwhile, three independent equations are constructed and extracted by placing two SVTMs in different locations. By solving these three equations, the rotor position can be calculated directly with zero phase shift, which eliminates the speed iteration operation and improves the system’s dynamic performance. Furthermore, by analyzing the influences of machine parameters’ variations, the suitable virtual q‐axis inductance can be selected to quickly achieve the optimal‐efficiency sensorless control of the IPMSM. Finally, the experimental results on an IPMSM demonstrate that the rotor position with good steady‐state and dynamic performance can be obtained accurately by using the proposed sensorless control strategy.
Table S7 from A Compound That Inhibits Glycolysis in Prostate Cancer Controls Growth of Advanced Prostate Cancer
Authors: Uo Takuma , Ojo Kayode K. , Sprenger Cynthia C.T. , Epilepsia Kathryn S. , Perera B. Gayani K. , Damodarasamy Mamatha , Sun Shihua , Kim Soojin , Hogan Hannah H. , Hulverson Matthew A. , Choi Ryan , Whitman Grant R. , Barrett Lynn K. , Michaels Samantha A. , Xu Linda H. , Sun Vicky L. , Arnold Samuel L.M. , Pang Haley J. , Nguyen Matthew M. , Vigil Anna-Lena B.G. , Kamat Varun , Sullivan Lucas B. , Sweet Ian R. , Vidadala Ram , Maly Dustin J. , Van Voorhis Wesley C. , Plymate Stephen R.
Published: July 2024

Table S7 shows pharmacokinetic parameters after a single dose in mouse, rat, dog, and cynomolgus monkey.
Supplementary Figure S3 from The Novel ATR Inhibitor Tuvusertib (M1774) Induces Replication Protein Overexpression and Broad Synergy with DNA-targeted Anticancer Drugs
Authors: Jo Ukhyun , Arakawa Yasuhiro , Zimmermann Astrid , Taniyama Daiki , Mizunuma Makito , Jenkins Lisa M. , Maity Tapan K. , Kumar Suresh , Zenke Frank T. , Takebe Naoko , Pommier Yves
Published: July 2024

This figure shows protein networks in SN-38/control and correlation plots between replication fork protection proteins and VE-822.
Figure S4 from A Compound That Inhibits Glycolysis in Prostate Cancer Controls Growth of Advanced Prostate Cancer
Authors: Uo Takuma , Ojo Kayode K. , Sprenger Cynthia C.T. , Epilepsia Kathryn S. , Perera B. Gayani K. , Damodarasamy Mamatha , Sun Shihua , Kim Soojin , Hogan Hannah H. , Hulverson Matthew A. , Choi Ryan , Whitman Grant R. , Barrett Lynn K. , Michaels Samantha A. , Xu Linda H. , Sun Vicky L. , Arnold Samuel L.M. , Pang Haley J. , Nguyen Matthew M. , Vigil Anna-Lena B.G. , Kamat Varun , Sullivan Lucas B. , Sweet Ian R. , Vidadala Ram , Maly Dustin J. , Van Voorhis Wesley C. , Plymate Stephen R.
Published: July 2024

Figure S4 shows dose response of multiple cancer cell lines to BKIDC-1553-N-Me and 1817.
Figure S11 from A Compound That Inhibits Glycolysis in Prostate Cancer Controls Growth of Advanced Prostate Cancer
Authors: Uo Takuma , Ojo Kayode K. , Sprenger Cynthia C.T. , Epilepsia Kathryn S. , Perera B. Gayani K. , Damodarasamy Mamatha , Sun Shihua , Kim Soojin , Hogan Hannah H. , Hulverson Matthew A. , Choi Ryan , Whitman Grant R. , Barrett Lynn K. , Michaels Samantha A. , Xu Linda H. , Sun Vicky L. , Arnold Samuel L.M. , Pang Haley J. , Nguyen Matthew M. , Vigil Anna-Lena B.G. , Kamat Varun , Sullivan Lucas B. , Sweet Ian R. , Vidadala Ram , Maly Dustin J. , Van Voorhis Wesley C. , Plymate Stephen R.
Published: July 2024

Figure S11 shows glucose uptake assays.
Unveiling Cellular Identities and Gene Expression Pathways in Overlapping Myocarditis and Myositis
Authors: Fankhauser Reilly G. , Johnson Douglas B. , Moslehi Javid J. , Balko Justin M.
Published: July 2024

Immune checkpoint therapies can drive antitumor responses and benefit patients but can also induce life-threatening immune-related adverse events such as myocarditis and myositis. These immune-related adverse events are rare but carry substantial morbidity and mortality. In this issue, Siddiqui and colleagues use single-cell RNA and T-cell receptor sequencing to identify novel cellular subsets and propose various mechanisms that could contribute to the pathogenesis of immune checkpoint inhibitor–associated myocarditis and myositis. These new insights should help move the field toward the development of improved treatment and prevention options, ultimately improving patient outcomes.

See related article by Siddiqui et al., p. 964 (1)
The Effectiveness of the webinar in providing non-traumatic urology emergencies education to medical personnel during COVID-19 pandemic
Authors: Seputra Kurnia Penta , Medianto Medianto , Naim Hamdan Yuwafi , S.L. Muhammad Adi , Daryanto Besut
Published: July 2024

Objectives. Basic emergency knowledge is a very important skill for medical personnel. Providing medical information and education is the new challenge in the COVID-19 pandemic situation. Webinar should be considered as one of alternatives to provide and share the knowledge. This study assessed the effectiveness of webinar as a medium to provide information and education in the field of non-traumatic urology emergencies.Methods. A descriptive analytic research design with cross sectional approach was conducted for all participants. The learning outcomes were assessed by comparing the pretest and the post test.Results. A total of 775 participants were included in this study. It showed a significant improvement in average score from pre-test to post-test (p<0.000). It also showed a similar result in almost all of the topics with p<0.005, except for the topic of treatment of urinary retention with p=0.434.Conclusions. The webinar should be considered as one of the effective educational media to provide information and education on non-traumatic urology emergencies for medical personnel during COVID-19 pandemic.
Characteristics of Clinical Finding in Child Diagnosed with Wilm’s Tumor: A Case Series
Authors: Patimura Muhammad Ilham Fauzan , Azis Abdul , Bakri Syarif
Published: July 2024

Wilm’s Tumor is the second most common intra-abdominal cancer of childhood, the fifth most common pediatric malignancy overall, and the most common renal tumor in children, representing 95% of all pediatric renal malignancies. However, Most of them present as an asymptomatic abdominal mass. Here we present two cases of Wilm’s tumor with different characteristics of clinical symptoms and ages. In Conclusion, Wilms tumor has a variety of clinical characteristics in each patient. The disease is also frequently found asymptomatically which leads to a delay in diagnosis. Screening for Wilms’ tumor is very important for children who have syndromes or birth defects known to be linked to this disease.
Characteristic of Urinary Tract Stone Patient in Sido Waras General Hospital Mojokerto
Authors: Daniputra Kristian Mohamad , Pratanu Antono , Kurniawan Putu Adi
Published: July 2024

Objectives. Urinary tract stones have been discovered since the ancient Egyptian period. In the eighteenth century, a Russian scientist, H.C.G von Struve, discovered a struvite stone whose formation was initiated by a urinary tract infection. The prevalence of kidney stone disease in Indonesia was 0,6% in 2013, and in the United States, the incidence of kidney stone disease was 116/100.000 people in 2000. This research aims to determine the characteristics of urinary tract stone patients treated in Sido Waras General Hospital Mojokerto, East Java.Methods. The design of this research is a descriptive study. The inclusion criteria are all new cases of urinary tract stones treated in Sido Waras General Hospital between 2017 and 2018. All clinical data regarding urinary tract stone disease is obtained from medical records retrospectively.Results. There are 285 new cases of urinary tract stones treated in Sido Waras General Hospital Mojokerto in 2 years, in which 236 cases (83%) are male and 49 (17%) are female. Most urinary tract stone patients are classified in the 40-60 age group, with 197 cases in two years. The most common urinary tract stone is kidney stone, with 147 cases (51%). In two years, open surgery is the most common management method for urinary tract stone disease (52%). Based on comorbid disease, there are 120 urinary tract stone patients (42%) suffering from hypertension and 84 urinary tract stone patients (29%) suffering from diabetes mellitus.Conclusions. The incidence of urinary tract stones in Sido Waras General Hospital is 285 cases. Urinary tract stones are more common in males, with a male-to-female ratio of 4:1. Most cases are kidney stones.
The Relationship Between Bicycle Saddles and The Incidence of Erectile Dysfunction In Men : A Systematic Review
Authors: Dono Bimo
Published: July 2024

Objectives. There is conflicting evidence about the relationship between bicycle saddle type and erectile dysfunction (ED). The main limitation of some previous studies is the lack of a validated measure of ED.Methods. We searched several databases from an English-language medical literature review that was made of publications in peer review journals, including published abstract presentations at major medical meetings, using a variety of search terms relating to “bicycle saddle” and “erectile dysfunction”.Results. After systematic evaluation of 359 studies, 7 studies met our inclusion criteria. In general, cycling significantly reduced perineal oxygen pressure levels (p<0.05). When comparing between studies, cycling more than 3 hours per week was an independent relative risk (RR=1.72) of moderate to severe ED. In a narrower saddle, when examining perineal oxygen pressure, there was a decrease, where it was found that p<0.001, where significantly a narrower saddle increased compression at the perineal saddle interface which disrupted penile hemodynamics.Conclusions. Limited evidence supports a positive correlation between narrower bicycle saddles and compression of the perineal arteries which causes occlusion of penile perfusion, penile blood flow and can lead to endothelial damage and causes erectile dysfunction. Long bike rides with seated positions might cause temporary penile blood flow reduction due to perineal artery pressure, resulting in decreased erectile function. However, this issue usually resolves quickly when pressure is relieved. Heterogeneity between studies suggests the need for further investigation of specific saddle types for cyclists and the measurement.
William Harvey e a circulação sanguínea: um diálogo com seus antecessores
Authors: Nunes Luana Beatriz Xavier
Published: July 2024

William Harvey (1578-1656) apresentou a doutrina da circulação do sangue em 1628, em sua obra De motu cordis, considerada seu trabalho mais importante. O tratado foi resultado de nove anos de observações e demonstrações anatômicas de animais e humanos e causou grande impacto entre médicos, filósofos e anatomistas do período, gerando diversos comentários e refutações, principalmente porque as ideias de Harvey sobre a circulação implicavam a quebra de autoridade e do conhecimento tradicional que era aceito na época. Entretanto, a doutrina da circulação do sangue foi resultado de um conhecimento construído desde a Antiguidade até o início do século XVII e apesar de a obra de Harvey ser considerada um marco na mudança do pensamento fisiológico e anatômico e devido a seu estilo de pensamento lógico e sua firmeza em refutar ideias de seus antecessores, é inegável a contribuição de seus antecessores na construção do conhecimento sobre a circulação sanguínea. Este trabalho tem por objetivo apresentar alguns dos pressupostos que constituíram a tradição médica do período e como essas contribuições influenciaram Harvey, seu método e suas conclusões a respeito da anatomia cardiovascular, movimentos do coração e movimento do sangue em sistema fechado.
Alterations of Placental Sodium in Preeclampsia: Trophoblast Responses
Authors: Mistry Hiten D. , Klossner Rahel , Scaife Paula J. , Eisele Nicole , Kurlak Lesia O. , Kallol Sampada , Albrecht Christiane , Gennari-Moser Carine , Briggs Louise V. , Broughton Pipkin Fiona , Mohaupt Markus G.
Published: July 2024

BACKGROUND:

Evidence suggests that increasing salt intake in pregnancy lowers blood pressure, protecting against preeclampsia. We hypothesized that sodium (Na
+
) evokes beneficial placental signals that are disrupted in preeclampsia.

METHODS:

Blood and urine were collected from nonpregnant women of reproductive age (n=26) and pregnant women with (n=50) and without (n=55) preeclampsia, along with placental biopsies. Human trophoblast cell lines and primary human trophoblasts were cultured with varying Na
+
concentrations.

RESULTS:

Women with preeclampsia had reduced placental and urinary Na
+
concentrations, yet increased urinary angiotensinogen and reduced active renin, aldosterone concentrations, and osmotic response signal TonEBP (tonicity-responsive enhancer binding protein) expression. In trophoblast cell cultures, TonEBP was consistently increased upon augmented Na
+
exposure. Mechanistically, inhibiting Na
+
/K
+
-ATPase or adding mannitol evoked the TonEBP response, whereas inhibition of cytoskeletal signaling abolished it.

CONCLUSIONS:

Enhanced Na
+
availability induced osmotic gradient-dependent cytoskeletal signals in trophoblasts, resulting in proangiogenic responses. As placental salt availability is compromised in preeclampsia, adverse systemic responses are thus conceivable.
Air Pollution and Stroke: What Nurses Need to Know
Authors: Kloster Mary Eileen
Published: July 2024

Air pollution exposure is linked to an increased risk of stroke. Elevated levels of pollution (carbon monoxide, carbon dioxide, nitrous oxide, sulfur dioxide, coarse particulate matter [PM
10
], and especially fine particulate matter [PM
2.5
]) cause systemic inflammation after the particles are inhaled and lodge into lung tissue, causing an increased incidence of stroke, hospitalizations for stroke, and stroke mortality. Until air pollution levels are remediated, assessing Air Quality Index (AQI) and following the guidelines to decrease illness from exposure is imperative. AQI levels are reported hourly, identifying ambient PM
2.5
and ozone levels. When AQI levels are low, the risk of exposure to PM
2.5
is low. As the AQI increases, there is more risk. It is important to take steps to decrease exposure to PM
2.5
, especially for those with cardiovascular comorbidities such as diabetes and those with previous stroke events. This is important information for nurses to understand and share with their patients as a risk reduction strategy.
Dziewiętnastowieczne materiały gwarowe z obszaru Warmii, Mazur i terenów sąsiednich. Cz. 1
Authors: Rembiszewska Dorota Krystyna , Siatkowski Janusz
Published: July 2024

This publication is the first part of a two-volume edition of materials concerning nineteenth-century dialects in Warmia, Mazuria and neighbouring areas, i.e. the Chełmno Land (Pol. ziemia chełmińska, Ger. Kulmer Land) and the Lubawa Land (Pol. ziemia lubawska, Ger. Löbauer Land). Part One contains material from 456 questionnaires collected for the German Linguistic Atlas (Deutscher Sprachatlas) in the following historical administrative districts: Węgorzewo (Argenburg: 1 questionnaire), Giżycko (Lötzen: 27 questionnaires), Mrągowo (Sensburg: 35), Pisz (Johannisburg: 41), Szczytno (Ortelsburg: 106), Nidzica (Neidenburg: 54), Reszel (Rößel: 10), Olsztyn (Allenstein: 50), Ostróda (Osterode: 60), Lubawa (Löbau: 52), Działdowo (Soldau: 7), Brodnica (Strasburg an der Drewenz: 13). Each questionnaire contains translations of forty German sentences (formulated by Georg Wenker, a librarian working in Marburg) into a given local dialect. The texts presented are a unique source for the study of Polish dialects in the nineteenth century. They are the only testimony of this kind showing the existence of Polish local dialects developing in the environment of the German language. It should be noted that, as specified in comments to the questionnaires, Polish was the dominant or the only language used in many surveyed villages. The material was recorded by local teachers and its value varies between different questionnaires. Considering that the specificity of particular local dialects was conveyed in an unprofessional way, without using phonetic notation, some phonetic features are difficult to determine. On the other hand, the interpretation of morphological forms is quite clear and they are certainly very useful for linguistic studies. It is particularly worth noting the richness of archaic dialectal lexis and the level of its preservation.
How unique structural adaptations support and coordinate the complex function of von Willebrand factor
Authors: Lenting Peter J. , Denis Cécile V. , Christophe Olivier D.
Published: July 2024

von Willebrand factor (VWF) is a multimeric protein consisting of covalently linked monomers, which share an identical domain architecture. Although involved in processes such as inflammation, angiogenesis, and cancer metastasis, VWF is mostly known for its role in hemostasis, by acting as a chaperone protein for coagulation factor VIII (FVIII) and by contributing to the recruitment of platelets during thrombus formation. To serve its role in hemostasis, VWF needs to bind a variety of ligands, including FVIII, platelet-receptor glycoprotein Ib-α, VWF-cleaving protease ADAMTS13, subendothelial collagen, and integrin α-IIb/β-3. Importantly, interactions are differently regulated for each of these ligands. How are these binding events accomplished and coordinated? The basic structures of the domains that constitute the VWF protein are found in hundreds of other proteins of prokaryotic and eukaryotic organisms. However, the determination of the 3-dimensional structures of these domains within the VWF context and especially in complex with its ligands reveals that exclusive, VWF-specific structural adaptations have been incorporated in its domains. They provide an explanation of how VWF binds its ligands in a synchronized and timely fashion. In this review, we have focused on the domains that interact with the main ligands of VWF and discuss how elucidating the 3-dimensional structures of these domains has contributed to our understanding of how VWF function is controlled. We further detail how mutations in these domains that are associated with von Willebrand disease modulate the interaction between VWF and its ligands.
A phase 1 clinical trial of NKTR-255 with CD19-22 CAR T-cell therapy for refractory B-cell acute lymphoblastic leukemia
Authors: Srinagesh Hrishikesh , Jackson Clayton , Shiraz Parveen , Jeyakumar Nikeshan , Hamilton Mark , Egeler Emily , Mavroukakis Sharon , Kuo Adam , Cancilla Juancarlos , Sahaf Bita , Agarwal Neha , Kanegai Alyssa , Kramer Anne Marijn , Arai Sally , Bharadwaj Sushma , Dahiya Saurabh , Hosoya Hitomi , Johnston Laura , Kennedy Vanessa , Liedtke Michaela , Lowsky Robert , Mikkilineni Lekha , Negrin Robert , Rezvani Andrew , Sidana Surbhi , Shizuru Judith , Smith Melody , Weng Wen-Kai , Feldman Steven , Frank Matthew J. , Lee Zachary , Tagliaferri Mary , Marcondes A. Mario , Miklos David , Mackall Crystal , Muffly Lori
Published: July 2024

Although chimeric antigen receptor (CAR) T-cell (CAR-T) therapy has revolutionized the treatment of B-cell malignancies, many patients relapse and therefore strategies to improve antitumor immunity are needed. We previously designed a novel autologous bispecific CAR targeting CD19 and CD22 (CAR19-22), which was well tolerated and associated with high response rates but relapse was common. Interleukin-15 (IL15) induces proliferation of diverse immune cells and can augment lymphocyte trafficking. Here, we report the results of a phase 1 clinical trial of the first combination of a novel recombinant polymer-conjugated IL15 receptor agonist (NKTR-255), with CAR19-22, in adults with relapsed/refractory B-cell acute lymphoblastic leukemia. Eleven patients were enrolled, 9 of whom successfully received CAR19-22 followed by NKTR-255. There were no dose-limiting toxicities, with transient fever and myelosuppression as the most common possibly related toxicities. We observed favorable efficacy with 8 of 9 patients (89%) achieving measurable residual disease–negative remission. At 12 months, progression-free survival for NKTR-255 was double that of historical controls (67% vs 38%). We performed correlative analyses to investigate the effects of IL15 receptor agonism. Cytokine profiling showed significant increases in IL15 and the chemokines CXCL9 and CXCL10. The increase in chemokines was associated with decreases in absolute lymphocyte counts and CD8+ CAR T cells in the blood and 10-fold increases in cerebrospinal fluid CAR-T cells, suggesting lymphocyte trafficking to tissue. Combining NKTR-255 with CAR19-22 was safe, feasible, and associated with high rates of durable responses. This trial was registered at www.clinicaltrials.gov as #NCT03233854.
Novel MAGIC composite scores using both clinical symptoms and biomarkers best predict treatment outcomes of acute GVHD
Authors: Akahoshi Yu , Spyrou Nikolaos , Weber Daniela , Aguayo-Hiraldo Paibel , Ayuk Francis , Chanswangphuwana Chantiya , Choe Hannah K. , Eder Matthias , Etra Aaron M. , Grupp Stephan A. , Hexner Elizabeth O. , Hogan William J. , Kitko Carrie L. , Kraus Sabrina , Al Malki Monzr M. , Merli Pietro , Qayed Muna , Reshef Ran , Schechter Tal , Ullrich Evelyn , Vasova Ingrid , Wölfl Matthias , Zeiser Robert , Baez Janna , Beheshti Rahnuma , Eng Gilbert , Gleich Sigrun , Katsivelos Nikolaos , Kowalyk Steven , Morales George , Young Rachel , Chen Yi-Bin , Nakamura Ryotaro , Levine John E. , Ferrara James L. M.
Published: July 2024

Acute graft-versus-host disease (GVHD) grading systems that use only clinical symptoms at treatment initiation such as the Minnesota risk identify standard and high-risk categories but lack a low-risk category suitable to minimize immunosuppressive strategies. We developed a new grading system that includes a low-risk stratum based on clinical symptoms alone and determined whether the incorporation of biomarkers would improve the model’s prognostic accuracy. We randomly divided 1863 patients in the Mount Sinai Acute GVHD International Consortium (MAGIC) who were treated for GVHD into training and validation cohorts. Patients in the training cohort were divided into 14 groups based on similarity of clinical symptoms and similar nonrelapse mortality (NRM); we used a classification and regression tree (CART) algorithm to create three Manhattan risk groups that produced a significantly higher area under the receiver operating characteristic curve (AUC) for 6-month NRM than the Minnesota risk classification (0.69 vs 0.64, P = .009) in the validation cohort. We integrated serum GVHD biomarker scores with Manhattan risk using patients with available serum samples and again used a CART algorithm to establish 3 MAGIC composite scores that significantly improved prediction of NRM compared to Manhattan risk (AUC, 0.76 vs 0.70, P = .010). Each increase in MAGIC composite score also corresponded to a significant decrease in day 28 treatment response (80% vs 63% vs 30%, P < .001). We conclude that the MAGIC composite score more accurately predicts response to therapy and long-term outcomes than systems based on clinical symptoms alone and may help guide clinical decisions and trial design.
An αIIbβ3 monoclonal antibody traps a semiextended conformation and allosterically inhibits large ligand binding
Authors: Wang Lu , Wang Jialing , Li Jihong , Walz Thomas , Coller Barry S.
Published: July 2024

Monoclonal antibodies (mAbs) have provided valuable information regarding the structure and function of platelet αIIbβ3. Protein disulfide isomerase (PDI) has been implicated in αIIbβ3 activation and binds to thrombin-activated αIIbβ3. Using human platelets as the immunogen, we identified a new mAb (R21D10) that inhibits the binding of PDI to platelets activated with thrombin receptor–activating peptide (T6). R21D10 also partially inhibited T6-induced fibrinogen and PAC-1 binding to platelets, as well as T6- and adenosine 5'-diphosphate–induced platelet aggregation. Mutual competition experiments showed that R21D10 does not inhibit the binding of mAbs 10E5 (anti-αIIb cap domain) or 7E3 (anti-β3 β-I domain), and immunoblot studies indicated that R21D10 binds to β3. The dissociation of αIIbβ3 by EDTA had a minimal effect on R21D10 binding. Cryogenic electron microscopy of the αIIbβ3-R21D10 Fab complex revealed that R21D10 binds to the β3 integrin-epidermal growth factor 1 (I-EGF1) domain and traps an intermediate conformation of αIIbβ3 with semiextended leg domains. The binding of R21D10 produces a major structural change in the β3 I-EGF2 domain associated with a new interaction between the β3 I-EGF2 and αIIb thigh domains, which may prevent the swing-out motion of the β3 hybrid domain required for high-affinity ligand binding and protect αIIbβ3 from EDTA-induced dissociation. R21D10 partially reversed the ligand binding priming effect of eptifibatide, suggesting that it could convert the swung-out conformation into a semiextended conformation. We concluded that R21D10 inhibits ligand binding to αIIbβ3 via a unique allosteric mechanism, which may or may not be related to its inhibition of PDI binding.
Assessing the dynamics of forest ecosystem services to define forest use suitability: a case study of Pinus sylvestris in Spain
Authors: Krsnik Goran , Reynolds Keith M. , Aquilué Núria , Mola-Yudego Blas , Pecurul-Botines Mireia , Garcia-Gonzalo Jordi , González Olabarria José Ramón
Published: July 2024

Adopting a multi-criteria approach in forest management is essential for preserving or improving specific benefits while minimizing negative environmental impacts. Determining the appropriate long-term management approach for a forest requires considering heterogeneous environmental and social factors, as well as changes in forest characteristics over time. Conducting a strategic assessment of forest use suitability (FUS), namely productive, protective, conservation-oriented, social and multifunctional, at the national level, taking into account the dynamics in the provision of forest ecosystem services and the trade-offs between FUS alternatives, can guide the development of customized management strategies and policies that align with the specific requirements and conditions of the forest. In this study, we evaluate the supply and simulation-based changes over time of diverse ecosystem services of Pinus sylvestris stands in Spain and utilize a decision model to determine the most suitable FUS alternative that enhances the provision of these services. The assignment of the most appropriate FUS alternative aims to help in decision-making processes and in the selection of the most adequate management strategies. To achieve this, we utilize the last version of ecosystem management decision support (EMDS) system, a spatially focused decision support tool capable of generating precise results for multi-criteria assessment. Participatory planning actions based on Delphi principles and Analytic Hierarchy Process (AHP) analysis were applied and combined with geospatial logic-based modelling. According to the results, the dominant FUS is protective, followed by productive alternative, exhibiting high levels of multifunctionality.
Deepvirusclassifier: a deep learning tool for classifying SARS-CoV-2 based on viral subtypes within the coronaviridae family
Authors: Azevedo Karolayne S. , de Souza Luísa C. , Coutinho Maria G. F. , de M. Barbosa Raquel , Fernandes Marcelo A. C.
Published: July 2024

Purpose

In this study, we present DeepVirusClassifier, a tool capable of accurately classifying Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) viral sequences among other subtypes of the coronaviridae family. This classification is achieved through a deep neural network model that relies on convolutional neural networks (CNNs). Since viruses within the same family share similar genetic and structural characteristics, the classification process becomes more challenging, necessitating more robust models. With the rapid evolution of viral genomes and the increasing need for timely classification, we aimed to provide a robust and efficient tool that could increase the accuracy of viral identification and classification processes. Contribute to advancing research in viral genomics and assist in surveilling emerging viral strains.

Methods

Based on a one-dimensional deep CNN, the proposed tool is capable of training and testing on the Coronaviridae family, including SARS-CoV-2. Our model’s performance was assessed using various metrics, including F1-score and AUROC. Additionally, artificial mutation tests were conducted to evaluate the model’s generalization ability across sequence variations. We also used the BLAST algorithm and conducted comprehensive processing time analyses for comparison.

Results

DeepVirusClassifier demonstrated exceptional performance across several evaluation metrics in the training and testing phases. Indicating its robust learning capacity. Notably, during testing on more than 10,000 viral sequences, the model exhibited a more than 99% sensitivity for sequences with fewer than 2000 mutations. The tool achieves superior accuracy and significantly reduced processing times compared to the Basic Local Alignment Search Tool algorithm. Furthermore, the results appear more reliable than the work discussed in the text, indicating that the tool has great potential to revolutionize viral genomic research.

Conclusion

DeepVirusClassifier is a powerful tool for accurately classifying viral sequences, specifically focusing on SARS-CoV-2 and other subtypes within the Coronaviridae family. The superiority of our model becomes evident through rigorous evaluation and comparison with existing methods. Introducing artificial mutations into the sequences demonstrates the tool’s ability to identify variations and significantly contributes to viral classification and genomic research. As viral surveillance becomes increasingly critical, our model holds promise in aiding rapid and accurate identification of emerging viral strains.
Maternal outcomes of conservative management and cesarean hysterectomy for placenta accreta spectrum disorders: a systematic review and meta-analysis
Authors: Pan Siman , Han Minmin , Zhai Tianlang , Han Yufei , Lu Yihan , Huang Shiyun , Zuo Qing , Jiang Ziyan , Ge Zhiping
Published: July 2024

Background

Cesarean hysterectomy as a traditional therapeutic maneuver for placenta accreta spectrum (PAS) has been associated with serious morbidity, conservative management has been used in many institutions to treat women with PAS. This systematic review aims to compare maternal outcomes according to conservative management or cesarean hysterectomy in women with placenta accreta spectrum disorders.

Methods

A systematic literature search was performed in MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials, Web of Science, and four Chinese databases (Chinese Biomedical Literature Database, China National Knowledge Infrastructure, Chinese Wanfang database and VIP database) to May 2024. Included studies were to be retrospective or prospective in design and compare and report relevant maternal outcomes according to conservative management (the placenta left partially or totally in situ) or cesarean hysterectomy in women with PAS. A risk ratio (RR) with 95% confidence interval (95% CI) was calculated for categorical outcomes and weighted mean difference (WMD) with 95% CI for continuous outcomes. The Newcastle-Ottawa Quality Assessment Scale was used to assess the observational studies. All analyses were performed using STATA version 18.0.

Results

Eight studies were included in the meta-analysis. Compared with cesarean hysterectomy, PAS women undergoing conservative management showed lower estimated blood loss [WMD − 1623.83; 95% CI: -2337.87, -909.79], required fewer units of packed red blood cells [WMD − 2.37; 95% CI: -3.70, -1.04] and units of fresh frozen plasma transfused [WMD − 0.40; 95% CI: -0.62, -0.19], needed a shorter mean operating time [WMD − 73.69; 95% CI: -90.52, -56.86], and presented decreased risks of bladder injury [RR 0.24; 95% CI: 0.11, 0.50], ICU admission [RR 0.24; 95% CI: 0.11, 0.52] and coagulopathy [RR 0.20; 95% CI: 0.06, 0.74], but increased risk for endometritis [RR 10.91; 95% CI: 1.36, 87.59] and readmission [RR 8.99; 95% CI: 4.00, 12.21]. The incidence of primary or delayed hysterectomy rate was 25% (95% CI: 19–32, I2 = 40.88%) and the use of uterine arterial embolization rate was 78% (95% CI: 65–87, I2 = 48.79%) in conservative management.

Conclusion

Conservative management could be an effective alternative to cesarean hysterectomy when women with PAS desire to preserve the uterus and are informed about the limitations of conservative management.

Prospero ID

CRD42023484578.
Intrauterine fetal death due to rupture of umbilical vessels: a rare case of furcate cord insertion
Authors: Xu Heng , Lu Jia-Ping , Xu Qiu-Lian
Published: July 2024

Furcate cord insertion refers to the separation of umbilical vessels before reaching the placenta, where the branching vessels normally attach at the edge of the placental parenchyma or near the placental membranes. This is an extremely rare abnormal umbilical cord insertion. This paper reported a case of a furcate cord insertion, where the rupture of exposed umbilical vessels led to intrauterine fetal death at full term. Through literature review, we analyzed the prenatal ultrasound characteristics and pregnancy outcomes of furcate cord insertions, with the aim to improve detection rates and reduce the risk of adverse pregnancy outcomes.
Case report of a child with nephronophthisis from South Africa
Authors: Bhimma Rajendra , Jembere Edgar , Hariparshad Sudesh
Published: July 2024

Background

Nephronophthisis (NPHP) is an autosomal recessive disorder with a subset of patients presenting with extrarenal manifestations such as retinal degeneration, cerebella ataxia, liver fibrosis, skeletal abnormalities, cardiac malformations, and lung bronchiectasis. However, the involvement of other organ systems has also been documented. Extrarenal manifestations occur in approximately 10–20% of patients. In developed countries, it has been reported as one of the most common causes of monogenic chronic kidney failure (CKF) during the first three decades of life, with more than 25 genes associated with this condition. The current treatment options for managing NPHP include supportive care, management of complications, and kidney replacement therapy when necessary.

The index patient is a 10-year-old Caucasian female who presented with recurrent attacks of abdominal pain. Her elder sister, TN, who was 17 years old, was diagnosed with CKF and noted to have persistently elevated liver enzymes (gamma-glutamyl transferase, alanine, and aspartate transaminases). Following genetic testing, her elder sister was shown to have Nephronophthisis Type 3, and a liver biopsy showed early fibrotic changes. Subsequent genetic testing confirmed the index patient as having NPHP Type 3. A kidney biopsy showed focal sclerosed glomeruli with patchy areas of tubular atrophy and related tubulointerstitial changes in keeping with NPHP. We present the first confirmatory case of NPHP from South Africa based on histopathology and genetic testing in a 10-year-old Caucasian female who presented with recurrent attacks of abdominal pain, whose elder sister also presented with CKF and early liver fibrosis, confirmed on biopsy and genetic testing.

Conclusion

In low-middle-income countries, genetic testing should be undertaken whenever possible to confirm the diagnosis of NPHP, especially in those with a suggestive biopsy or if there is CKF of unknown aetiology with or without extra-renal manifestations.