bluuebunny/crossref_metadata_2025 huggingface dataset:
1-C Metabolism—Serine, Glycine, Folates—In Acute Myeloid Leukemia
Authors: Mahmood Kanwal , Emadi Ashkan
Published: February 2021

Metabolic reprogramming contributes to tumor development and introduces metabolic liabilities that can be exploited to treat cancer. Studies in hematological malignancies have shown alterations in fatty acid, folate, and amino acid metabolism pathways in cancer cells. One-carbon (1-C) metabolism is essential for numerous cancer cell functions, including protein and nucleic acid synthesis and maintaining cellular redox balance, and inhibition of the 1-C pathway has yielded several highly active drugs, such as methotrexate and 5-FU. Glutamine depletion has also emerged as a therapeutic approach for cancers that have demonstrated dependence on glutamine for survival. Recent studies have shown that in response to glutamine deprivation leukemia cells upregulate key enzymes in the serine biosynthesis pathway, suggesting that serine upregulation may be a targetable compensatory mechanism. These new findings may provide opportunities for novel cancer treatments.
Morin-5′-Sulfonic Acid Sodium Salt (NaMSA) Attenuates Cyclophosphamide-Induced Histological Changes in Genitourinary Tract in Rats—Short Report
Authors: Merwid-Ląd Anna , Ksiądzyna Dorota , Hałoń Agnieszka , Szkudlarek Danuta , Trocha Małgorzata , Szandruk-Bender Marta , Matuszewska Agnieszka , Nowak Beata , Sozański Tomasz , Kuźniar Anna , Szeląg Adam
Published: February 2021

Cyclophosphamide (CPX) exerts toxicity in the urogenital system. The current study was designed to evaluate the effect of morin-5′-sulfonic acid sodium salt (NaMSA) on CPX-induced urogenital toxicity in rats. NaMSA (100 mg/kg/daily) and CPX (15 mg/kg/daily) alone or in combination and 0.9% NaCl (as a control) were given intragastrically for 10 days. Testes and epididymes from male and urinary bladders from male and female rats were evaluated histologically. In testes and epididymes, morphological changes and relative decrease in sperm count were assessed. In urinary bladders edema, hemorrhage and urothelium erosions were described by 0–2 points scoring system. Reproductive score (RS—in total 6 points) and urinary bladder score (BS—in total 6 points) were thereafter calculated. In CPX-receiving group RS (2.7) and BS (3.3) were significantly higher than in the control (0.5 and 0.25 for RS and BS, respectively). Co-administration of NaMSA reversed most of the morphological changes, which was reflected by lower RS and BS score (0.5 and 1.2 for RS and BS, respectively). The preliminary findings suggest that NaMSA may attenuate CPX-induced histological changes in rat urogenital tract.
A Design Approach of Optical Phased Array with Low Side Lobe Level and Wide Angle Steering Range
Authors: He Xinyu , Dong Tao , He Jingwen , Xu Yue
Published: February 2021

In this paper, a new design approach of optical phased array (OPA) with low side lobe level (SLL) and wide angle steering range is proposed. This approach consists of two steps. Firstly, a nonuniform antenna array is designed by optimizing the antenna spacing distribution with particle swarm optimization (PSO). Secondly, on the basis of the optimized antenna spacing distribution, PSO is further used to optimize the phase distribution of the optical antennas when the beam steers for realizing lower SLL. Based on the approach we mentioned, we design a nonuniform OPA which has 1024 optical antennas to achieve the steering range of ±60°. When the beam steering angle is 0°, 20°, 30°, 45° and 60°, the SLL obtained by optimizing phase distribution is −21.35, −18.79, −17.91, −18.46 and −18.51 dB, respectively. This kind of OPA with low SLL and wide angle steering range has broad application prospects in laser communication and lidar system.
LIDAR and Beam Steering Tailored by Neuromorphic Metasurfaces Dipped in a Tunable Surrounding Medium
Authors: Lio Giuseppe Emanuele , Ferraro Antonio
Published: February 2021

The control of amplitude, losses and deflection of light with elements of an optical array is of paramount importance for realizing dynamic beam steering for light detection and ranging applications (LIDAR). In this paper, we propose an optical beam steering device, operating at a wavelength of 1550 nm, based on high index material as molybdenum disulfide (MoS2) where the direction of the light is actively controlled by means of liquid crystal. The metasurface have been designed by a deep machine learning algorithm jointed with an optimizer in order to obtain univocal optical responses. The achieved numerical results represent a promising way for the realization of novel LIDAR for future applications with increase control and precision.
Unusual Polarization Relation between Single-Mode Lasing Emission and Excitation Laser from an Evanescent-Wave Pumped Micro-Cavity Laser
Authors: Wang Yuchen , Li Hongsheng , Hu Shu , Li Heng , Sheng Chuanxiang
Published: February 2021

Using a fiber of that is 125 μm in diameter in rhodamine 6G ethanol solution, controllable multi- and single-whispering-gallery-mode (WGM) optofluidic lasers based on evanescent-wave-coupled gain are both available. With multi-mode WGM emission, lasing emission with almost pure TM (transverse magnetic) or almost TE (transverse electric) modes can be obtained when the pump laser has an electric field parallel (perpendicular) to the fiber axis, i.e., the polarization direction of output laser is the same as that of the pump laser. On the other hand, when the laser emission is single-mode, the TE output laser always emerges firstly above lasing threshold, then keeps TE mode while the pump laser’s intensity increases with polarization direction perpendicular to the fiber axis; on the contrary, TE emission will dwindle relatively, while the TM emission emerges and dominates the spectra, when the pump laser’s intensity increases with polarization parallel to the fiber axis. Our work proves that controlling the leakage of the evanescent wave from high-Q microcavities is crucial for both modes of lasing emission and its polarization.
Peak-to-Average Power Ratio Reduction of Carrier-Suppressed Optical SSB Modulation: Performance Comparison of Three Methods
Authors: Sampath K. I. Amila , Takano Katsumi , Maeda Joji
Published: February 2021

We compare the performances of three previously proposed methods to reduce the peak-to-average power ratio (PAPR) of the carrier-suppressed optical single-sideband (OSSB-SC) signal. PAPR of OSSB-SC signal becomes high due to the peaky Hilbert-transformed signal which is used for spectral suppression. Nonlinear phase shifts induced by high PAPR degrade OSSB-SC signal during fiber transmission. Previously, we proposed peak folding, peak clipping, and high-pass Hilbert transform methods to reduce the PAPR of OSSB-SC modulation. In this study, we numerically compare the effectiveness of proposed methods in a 10 Gbit/s non-return-to-zero (NRZ)-coded 100-km single-channel transmission link. Due to the reduced PAPR, peak folding and peak clipping can increase the self-phase modulation (SPM) threshold of the studied system by 2.40 dB and 2.63 dB respectively. The high-pass Hilbert transform method improves the SPM threshold by more than 9 dB.
Fantastic Downy Mildew Pathogens and How to Find Them: Advances in Detection and Diagnostics
Authors: Salcedo Andres F. , Purayannur Savithri , Standish Jeffrey R. , Miles Timothy , Thiessen Lindsey , Quesada-Ocampo Lina M.
Published: February 2021

Downy mildews affect important crops and cause severe losses in production worldwide. Accurate identification and monitoring of these plant pathogens, especially at early stages of the disease, is fundamental in achieving effective disease control. The rapid development of molecular methods for diagnosis has provided more specific, fast, reliable, sensitive, and portable alternatives for plant pathogen detection and quantification than traditional approaches. In this review, we provide information on the use of molecular markers, serological techniques, and nucleic acid amplification technologies for downy mildew diagnosis, highlighting the benefits and disadvantages of the technologies and target selection. We emphasize the importance of incorporating information on pathogen variability in virulence and fungicide resistance for disease management and how the development and application of diagnostic assays based on standard and promising technologies, including high-throughput sequencing and genomics, are revolutionizing the development of species-specific assays suitable for in-field diagnosis. Our review provides an overview of molecular detection technologies and a practical guide for selecting the best approaches for diagnosis.
Antioxidant Enzymatic Activity and Osmotic Adjustment as Components of the Drought Tolerance Mechanism in Carex duriuscula
Authors: Hou Peichen , Wang Feifei , Luo Bin , Li Aixue , Wang Cheng , Shabala Lana , Ahmed Hassan Ahmed Ibraheem , Deng Shurong , Zhang Huilong , Song Peng , Zhang Yuhong , Shabala Sergey , Chen Liping
Published: February 2021

Drought stress is a major environmental constraint for plant growth. Climate-change-driven increases in ambient temperatures resulted in reduced or unevenly distributed rainfalls, leading to increased soil drought. Carex duriuscula C. A. Mey is a typical drought-tolerant sedge, but few reports have examined the mechanisms conferring its tolerant traits. In the present study, the drought responses of C. duriuscula were assessed by quantifying activity of antioxidant enzymes in its leaf and root tissues and evaluating the relative contribution of organic and inorganic osmolyte in plant osmotic adjustment, linking it with the patterns of the ion acquisition by roots. Two levels of stress—mild (MD) and severe (SD) drought treatments—were used, followed by re-watering. Drought stress caused reduction in a relative water content and chlorophyll content of leaves; this was accompanied by an increase in the hydrogen peroxide (H2O2) and superoxide (O2−) contents in leaves and roots. Under MD stress, the activities of catalase (CAT), peroxidase (POD), and glutathione peroxidase (GPX) increased in leaves, whereas, in roots, only CAT and POD activities increased. SD stress led to an increase in the activities of CAT, POD, superoxide dismutase (SOD), and GPX in both tissues. The levels of proline, soluble sugars, and soluble proteins in the leaves also increased. Under both MD and SD stress conditions, C. duriuscula increased K+, Na+, and Cl− uptake by plant roots, which resulted in an increased K+, Na+, and Cl− concentrations in leaves and roots. This reliance on inorganic osmolytes enables a cost-efficient osmotic adjustment in C. duriuscula. Overall, this study revealed that C. duriuscula was able to survive arid environments due to an efficient operation of its ROS-scavenging systems and osmotic adjustment mechanisms.
Priming Maritime Pine Megagametophytes during Somatic Embryogenesis Improved Plant Adaptation to Heat Stress
Authors: Pérez-Oliver María Amparo , Haro Juan Gregorio , Pavlović Iva , Novák Ondřej , Segura Juan , Sales Ester , Arrillaga Isabel
Published: February 2021

In the context of global climate change, forest tree research should be addressed to provide genotypes with increased resilience to high temperature events. These improved plants can be obtained by heat priming during somatic embryogenesis (SE), which would produce an epigenetic-mediated transgenerational memory. Thereby, we applied 37 °C or 50 °C to maritime pine (Pinus pinaster) megagametophytes and the obtained embryogenic masses went through the subsequent SE phases to produce plants that were further subjected to heat stress conditions. A putative transcription factor WRKY11 was upregulated in priming-derived embryonal masses, and also in the regenerated P37 and P50 plants, suggesting its role in establishing an epigenetic memory in this plant species. In vitro-grown P50 plants also showed higher cytokinin content and SOD upregulation, which points to a better responsiveness to heat stress. Heat exposure of two-year-old maritime pine plants induced upregulation of HSP70 in those derived from primed embryogenic masses, that also showed better osmotic adjustment and higher increases in chlorophyll, soluble sugars and starch contents. Moreover, ϕPSII of P50 plants was less affected by heat exposure. Thus, our results suggest that priming at 50 °C at the SE induction phase is a promising strategy to improve heat resilience in maritime pine.
Far-Infrared Emission Properties and Thermogravimetric Analysis of Ceramic-Embedded Polyurethane Films
Authors: Faisal Ashik Md , Salaün Fabien , Giraud Stéphane , Ferri Ada , Chen Yan , Wang Lichuan
Published: February 2021

The far-infrared ray (FIR) is one kind of electromagnetic wave employed for numerous bio-interactive applications such as body thermoregulation, infrared therapy, etc. Tuning the FIR-emitting property of the functional textile surface can initiate a new horizon to utilize this property in sportswear or even smart textiles. Ceramic particles were studied for their unique ability to constantly emit FIR rays. The purpose of this research is to characterize the FIR emission properties and the thermogravimetric analysis of ceramic-embedded polyurethane films. For this purpose, ceramic particles such as aluminum oxide, silicon dioxide, and titanium dioxide were incorporated (individually) with water-based polyurethane (WPU) binder by a sonication technique to make a thin layer of film. Significant improvement in FIR emissive property of the films was found when using different ceramic particles into the polyurethane films. Reflection and transmission at the FIR range were measured with a gold integrating sphere by Fourier-transform infrared (FTIR) spectrometer. The samples were also characterized by thermogravimetric analysis (TGA). Different physical tests, such as tensile strength and contact angle measurements, were performed to illustrate the mechanical properties of the films. The study suggested that the mechanical properties of the polyurethane films were significantly influenced by the addition of ceramic particles.
Characterization and Biotechnological Potential of Intracellular Polyhydroxybutyrate by Stigeoclonium sp. B23 Using Cassava Peel as Carbon Source
Authors: Mourão Murilo Moraes , Xavier Luciana Pereira , Urbatzka Ralph , Figueiroa Lucas Barbosa , Costa Carlos Emmerson Ferreira da , Dias Carmen Gilda Barroso Tavares , Schneider Maria Paula Cruz , Vasconcelos Vitor , Santos Agenor Valadares
Published: February 2021

The possibility of utilizing lignocellulosic agro-industrial waste products such as cassava peel hydrolysate (CPH) as carbon sources for polyhydroxybutyrate (PHB) biosynthesis and characterization by Amazonian microalga Stigeoclonium sp. B23. was investigated. Cassava peel was hydrolyzed to reducing sugars to obtain increased glucose content with 2.56 ± 0.07 mmol/L. Prior to obtaining PHB, Stigeoclonium sp. B23 was grown in BG-11 for characterization and Z8 media for evaluation of PHB nanoparticles’ cytotoxicity in zebrafish embryos. As results, microalga produced the highest amount of dry weight of PHB with 12.16 ± 1.28 (%) in modified Z8 medium, and PHB nanoparticles exerted some toxicity on zebrafish embryos at concentrations of 6.25–100 µg/mL, increased mortality (<35%) and lethality indicators as lack of somite formation (<25%), non-detachment of tail, and lack of heartbeat (both <15%). Characterization of PHB by scanning electron microscopy (SEM), X-ray diffraction (XRD), differential scanning calorimeter (DSC), and thermogravimetry (TGA) analysis revealed the polymer obtained from CPH cultivation to be morphologically, thermally, physically, and biologically acceptable and promising for its use as a biomaterial and confirmed the structure of the polymer as PHB. The findings revealed that microalgal PHB from Stigeoclonium sp. B23 was a promising and biologically feasible new option with high commercial value, potential for biomaterial applications, and also suggested the use of cassava peel as an alternative renewable resource of carbon for PHB biosynthesis and the non-use of agro-industrial waste and dumping concerns.
Insights on Cellulose Research in the Last Two Decades in Romania
Authors: Coseri Sergiu
Published: February 2021

In the current global context, cellulose fulfills those characteristics that give it clear advantages over synthetic fibers, having a huge potential for substituting fossil-based materials which are polluting and harmful to ecosystems. Research conducted in most laboratories around the world in the field of cellulose is overwhelmingly aimed at industrial needs because features such as renewability and low cost are the most important attributes for economic success. In this global effort, Romanian researchers contribute through achievements that are briefly reviewed in this paper. These refer to the main achievements reported after 2000 in the field of cellulose characterization and cellulose functionalization, as well as the main areas where cellulose-based materials were applied.
Influence of Treated Distillate Aromatic Extract (TDAE) Content and Addition Time on Rubber-Filler Interactions in Silica Filled SBR/BR Blends
Authors: Sökmen Selin , Oßwald Katja , Reincke Katrin , Ilisch Sybill
Published: February 2021

High compatibility and good rubber–filler interactions are required in order to obtain high quality products. Rubber–filler and filler–filler interactions can be influenced by various material factors, such as the presence of processing aids. Although different processing aids, especially the plasticizers, and their effects on compatibility have been investigated in the literature, their influence on rubber–filler interactions in highly active filler reinforced mixtures is not explicit and has not been investigated in depth. For this purpose, the influence of treated distillate aromatic extract (TDAE) oil content and its addition time on interactions between silica and rubber chains were investigated in this study. Rubber–filler and filler–filler interactions of uncured and cured silica-filled SBR/BR blends were characterized by using rubber layer L concept and dynamic mechanical analysis, whereas mechanical properties were studied by tensile test and Shore A hardness. Five parts per hundred rubber (phr) TDAE addition at 0, 1.5, and 3 min of mixing were characterized to investigate the influence of TDAE addition time on rubber–filler interactions. It was observed that addition time of TDAE can influence the development of bounded rubber structure and the interfacial interactions, especially at short time of mixing, less than 5 min. Oil addition with silica at 1.5 min of mixing resulted in fast rubber layer development and a small reduction in storage shear modulus of uncured blends. The influence of oil content on rubber–filler and filler–filler interactions were investigated for the binary blends without oil, with 5 and 20 phr TDAE content. The addition of 5 phr oil resulted in a slight increase in rubber layer and 0.05 MPa reduction in Payne effect of uncured blends. The storage tensile modulus of vulcanizates at small strains decreased from 13.97 to 8.28 MPa after oil addition. Twenty parts per hundred rubber (phr) oil addition to binary blends caused rubber layer L to decrease from 0.45 to 0.42. The storage tensile modulus of the vulcanizates and its reduction with higher amplitudes were incontrovertibly high among the vulcanizates with lower oil content, which were 13.57 and 4.49 MPa, respectively. When any consequential change in mechanical properties of styrene–butadiene rubber (SBR)/butadiene rubber (BR) blends could not be observed at different TDAE addition time, increasing amount of oil in blends enhanced elongation at break, and decreased Shore A hardness and tensile strength.
Fused Filament Fabrication-4D-Printed Shape Memory Polymers: A Review
Authors: Valvez Sara , Reis Paulo N. B. , Susmel Luca , Berto Filippo
Published: February 2021

Additive manufacturing (AM) is the process through which components/structures are produced layer-by-layer. In this context, 4D printing combines 3D printing with time so that this combination results in additively manufactured components that respond to external stimuli and, consequently, change their shape/volume or modify their mechanical properties. Therefore, 4D printing uses shape-memory materials that react to external stimuli such as pH, humidity, and temperature. Among the possible materials with shape memory effect (SME), the most suitable for additive manufacturing are shape memory polymers (SMPs). However, due to their weaknesses, shape memory polymer compounds (SMPCs) prove to be an effective alternative. On the other hand, out of all the additive manufacturing techniques, the most widely used is fused filament fabrication (FFF). In this context, the present paper aims to critically review all studies related to the mechanical properties of 4D-FFF materials. The paper provides an update state of the art showing the potential of 4D-FFF printing for different engineering applications, maintaining the focus on the structural integrity of the final structure/component.
Reinforced Universal Adhesive by Ribose Crosslinker: A Novel Strategy in Adhesive Dentistry
Authors: Bourgi Rim , Daood Umer , Bijle Mohammed Nadeem , Fawzy Amr , Ghaleb Maroun , Hardan Louis
Published: February 2021

Enzymatic biodegradation of demineralized collagen fibrils could lead to the reduction of resin–dentin bond strength. Therefore, methods that provide protection to collagen fibrils appear to be a pragmatic solution to improve bond strength. Thus, the study’s aim was to investigate the effect of ribose (RB) on demineralized resin–dentin specimens in a modified universal adhesive. Dentin specimens were obtained, standardized and then bonded in vitro with a commercial multi-mode adhesive modified with 0, 0.5%, 1%, and 2% RB, restored with resin composite, and tested for micro-tensile bond strength (µTBS) after storage for 24 h in artificial saliva. Scanning electron microscopy (SEM) was performed to analyze resin–dentin interface. Contact angles were analyzed using a contact angle analyzer. Depth of penetration of adhesives and nanoleakage were assessed using micro-Raman spectroscopy and silver tracing. Molecular docking studies were carried out using Schrodinger small-molecule drug discovery suite 2019-4. Matrix metalloproteinases-2 (MMP-2) and cathepsin-K activities in RB-treated specimens were quantified using enzyme-linked immunosorbent assay (ELISA). The significance level was set at α = 0.05 for all statistical analyses. Incorporation of RB at 1% or 2% is of significant potential (p < 0.05) as it can be associated with improved wettability on dentin surfaces (0.5% had the lowest contact angle) as well as appreciable hybrid layer quality, and higher resin penetration. Improvement of the adhesive bond strength was shown when adding RB at 1% concentration to universal adhesive (p < 0.05). Modified adhesive increased the resistance of collagen degradation by inhibiting MMP-2 and cathepsin-K. A higher RB concentration was associated with improved results (p < 0.01). D-ribose showed favorable negative binding to collagen. In conclusion, universal adhesive using 1% or 2% RB helped in maintaining dentin collagen scaffold and proved to be successful in improving wettability, protease inhibition, and stability of demineralized dentin substrates. A more favorable substrate is created which, in turn, leads to a more stable dentin-adhesive bond. This could lead to more advantageous outcomes in a clinical scenario where a stable bond may result in longevity of the dental restoration.
Thermal Stability Analysis of Lithium-Ion Battery Electrolytes Based on Lithium Bis(trifluoromethanesulfonyl)imide-Lithium Difluoro(oxalato)Borate Dual-Salt
Authors: Yang Ya-Ping , Huang An-Chi , Tang Yan , Liu Ye-Cheng , Wu Zhi-Hao , Zhou Hai-Lin , Li Zhi-Ping , Shu Chi-Min , Jiang Jun-Cheng , Xing Zhi-Xiang
Published: February 2021

Lithium-ion batteries with conventional LiPF6 carbonate electrolytes are prone to failure at high temperature. In this work, the thermal stability of a dual-salt electrolyte of lithium bis(trifluoromethanesulfonyl)imide (LiTFSI) and lithium difluoro(oxalato)borate (LiODFB) in carbonate solvents was analyzed by accelerated rate calorimetry (ARC) and differential scanning calorimetry (DSC). LiTFSI-LiODFB dual-salt carbonate electrolyte decomposed when the temperature exceeded 138.5 °C in the DSC test and decomposed at 271.0 °C in the ARC test. The former is the onset decomposition temperature of the solvents in the electrolyte, and the latter is the LiTFSI-LiODFB dual salts. Flynn-Wall-Ozawa, Starink, and autocatalytic models were applied to determine pyrolysis kinetic parameters. The average apparent activation energy of the dual-salt electrolyte was 53.25 kJ/mol. According to the various model fitting, the thermal decomposition process of the dual-salt electrolyte followed the autocatalytic model. The results showed that the LiTFSI-LiODFB dual-salt electrolyte is significantly better than the LiPF6 electrolyte in terms of thermal stability.
Experimental Study of Oriented Strand Board Ignition by Radiant Heat Fluxes
Authors: Tureková Ivana , Marková Iveta , Ivanovičová Martina , Harangózo Jozef
Published: February 2021

Wood and composite panel materials represent a substantial part of the fuel in many building fires. The ability of materials to ignite when heated at elevated temperatures depends on many factors, such as the thermal properties of materials, the ignition temperature, critical heat flux and the environment. Oriented strand board (OSB) without any surface treatment in thicknesses of 12, 15 and 18 mm were used as experimental samples. The samples were gradually exposed to a heat flux of 43 to 50 kW.m−2, with an increase of 1 kW.m−2. At heat fluxes of 49 kW.m−2 and 50 kW.m−2, the ignition times are similar in all OSB thicknesses, in contrast to the ignition times at lower heat fluxes. The influence of the selected factors (thickness and distance from the heat source) was analysed based on the experimentally obtained data of ignition time and weight loss. The experimentally determined value of the heat flux density was 43 kW.m−2, which represented the critical heat flux. The results show a statistically significant effect of OSB thickness on ignition time.
Polymers and Nanoparticles for Statin Delivery: Current Use and Future Perspectives in Cardiovascular Disease
Authors: Nenna Antonio , Nappi Francesco , Larobina Domenico , Verghi Emanuele , Chello Massimo , Ambrosio Luigi
Published: February 2021

Atherosclerosis-related coronary artery disease (CAD) is one of the leading sources of mortality and morbidity in the world. Primary and secondary prevention appear crucial to reduce CAD-related complications. In this scenario, statin treatment was shown to be clinically effective in the reduction of adverse events, but systemic administration provides suboptimal results. As an attempt to improve bioavailability and effectiveness, polymers and nanoparticles for statin delivery were recently investigated. Polymers and nanoparticles can help statin delivery and their effects by increasing oral bioavailability or enhancing target-specific interaction, leading to reduced vascular endothelial dysfunction, reduced intimal hyperplasia, reduced ischemia-reperfusion injury, increased cardiac regeneration, positive remodeling in the extracellular matrix, reduced neointimal growth and increased re-endothelization. Moreover, some innovative aspects described in other cardiovascular fields could be translated into the CAD scenario. Recent preclinical studies are underlining the effect of statins in the stimulation and differentiation of endogenous cardiac stem cells, as well as in targeting of local adverse conditions implicated in atherosclerosis, and statin delivery through poly-lactic-co-glycolic acid (PLGA) appears the most promising aspect of current research to enhance drug activity. The present review intends to summarize the current evidence about polymers and nanoparticles for statin delivery in the field of cardiovascular disease, trying to shed light on this topic and identify new avenues for future studies.
Effect of Chitosan Coating Incorporated with Artemisia fragrans Essential Oil on Fresh Chicken Meat during Refrigerated Storage
Authors: Yaghoubi Milad , Ayaseh Ali , Alirezalu Kazem , Nemati Zabihollah , Pateiro Mirian , Lorenzo José M.
Published: February 2021

The present study was conducted to assess the impact of chitosan coating (1%) containing Artemisia fragrans essential oil (500, 1000, and 1500 ppm) as antioxidant and antimicrobial agent on the quality properties and shelf life of chicken fillets during refrigerated storage. After packaging meat samples, physicochemical, microbiological, and organoleptic attributes were evaluated at 0, 3, 6, 9, and 12 days at 4 °C. The results revealed that applied chitosan (CH) coating in combination with Artemisia fragrans essential oils (AFEOs) had no significant (p < 0.05) effects on proximate composition among treatments. The results showed that the incorporation of AFEOs into CH coating significantly reduced (p < 0.05) pH, thiobarbituric acid reactive substances (TBARS), and total volatile base nitrogen (TVB-N), especially for 1% CH coating + 1500 ppm AFEOs, with values at the end of storage of 5.58, 1.61, and 2.53, respectively. The coated samples also displayed higher phenolic compounds than those obtained by uncoated samples. Coated chicken meat had, significantly (p < 0.05), the highest inhibitory effects against microbial growth. The counts of TVC (total viable counts), coliforms, molds, and yeasts were significantly lower (p < 0.05) in 1% CH coating + 1500 ppm AFEOs fillets (5.32, 3.87, and 4.27 Log CFU/g, respectively) at day 12. Organoleptic attributes of coated samples also showed the highest overall acceptability scores than uncoated ones. Therefore, the incorporation of AFEOs into CH coating could be effectively used for improving stability and shelf life of chicken fillets during refrigerated storage.
Biopolymeric Materials Used as Nonviral Vectors: A Review
Authors: Santos Jailson de Araújo , Liarte Daniel Barbosa , Ribeiro Alessandra Braga , Rizzo Marcia dos Santos , Costa Marcília Pinheiro da , Osajima Josy A. , Silva-Filho Edson C.
Published: February 2021

Bacterial transformation and gene transfection can be understood as being the results of introducing specific genetic material into cells, resulting in gene expression, and adding a new genetic trait to the host cell. Many studies have been carried out to investigate different types of lipids and cationic polymers as promising nonviral vectors for DNA transfer. The present study aimed to carry out a systematic review on the use of biopolymeric materials as nonviral vectors. The methodology was carried out based on searches of scientific articles and applications for patents published or deposited from 2006 to 2020 in different databases for patents (EPO, USPTO, and INPI) and articles (Scopus, Web of Science, and Scielo). The results showed that there are some deposits of patents regarding the use of chitosan as a gene carrier. The 16 analyzed articles allowed us to infer that the use of biopolymers as nonviral vectors is limited due to the low diversity of biopolymers used for these purposes. It was also observed that the use of different materials as nonviral vectors is based on chemical structure modifications of the material, mainly by the addition of cationic groups. Thus, the use of biopolymers as nonviral vectors is still limited to only a few polysaccharide types, emphasizing the need for further studies involving the use of different biopolymers in processes of gene transfer.
Creating Values from Biomass Pyrolysis in Sweden: Co-Production of H2, Biocarbon and Bio-Oil
Authors: Zaini Ilman Nuran , Sophonrat Nanta , Sjöblom Kurt , Yang Weihong
Published: February 2021

Hydrogen and biocarbon are important materials for the future fossil-free metallurgical industries in Sweden; thus, it is interesting to investigate the process that can simultaneously produce both. Process simulations of biomass pyrolysis coupled with steam reforming and water-gas-shift to produce H2, biocarbon, and bio-oil are investigated in this work. The process simulation is performed based on a biomass pyrolysis plant currently operating in Sweden. Two co-production schemes are proposed: (1) production of biocarbon and H2, and (2) production of biocarbon, H2, and bio-oil. Sensitivity analysis is also performed to investigate the performance of the production schemes under different operating parameters. The results indicated that there are no notable differences in terms of the thermal efficiency for both cases. Varying the bio-oil condenser temperature only slightly changes the system’s thermal efficiency by less than 2%. On the other hand, an increase in biomass moisture content from 7 to 14 wt.% can decrease the system’s efficiency from 79.0% to 72.6%. Operating expenses are evaluated to elucidate the economics of 3 different cases: (1) no bio-oil production, (2) bio-oil production with the condenser at 50 °C, and (3) bio-oil production with the condenser at 130 °C. Based on operation expenses (OPEX) and revenue alone, it is found that producing more bio-oil helps improving the economics of the process. However, capital costs and the cost for post-processing of bio-oil should also be considered in the future. The estimated minimum selling price for biocarbon based on OPEX alone is approx. 10 SEK, which is within the range of the current commercial price of charcoal and coke.
Removal of Anionic and Cationic Dyes from Wastewater Using Activated Carbon from Palm Tree Fiber Waste
Authors: Alhogbi Basma G. , Altayeb Shoruq , Bahaidarah Effat. A. , Zawrah Mahmoud F.
Published: February 2021

This study focuses on using a facile method for the green preparation of activated carbon (AC) from palm tree fiber (PTF) waste. The synthesized cost-effective AC was investigated for the removal of an anionic dye (Congo red, CR) and a cationic dye (Rhodamine B, RhB) from wastewater. The morphological and structural characterization of the synthesized AC were performed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), surface area, Fourier transform infrared spectroscopy (FTIR), total pore volume, average pore diameter and pore size distribution, zeta potential, and zero-point charge. To investigate the adsorption efficiency, different parameters such as adsorbent dosage, solution pH, initial dye concentration, and duration were applied using the batch experiments. Various adsorption isotherm and kinetics models were applied to study the adsorption mechanism and dynamics. The results showed that chemical activation with a weak acid (H3PO4) at 400 °C for 30 min is a fast method for the activation of each precursor and produces a high yield. The result of analysis showed an increase in the adsorption capacity at pH 2. The maximum adsorption capacity was 9.79 and 26.58 mg g−1 at 30 min for CR dye and RhB dye, respectively. The optimum adsorbent dosage for the activated carbon from palm tree fiber (PTFAC) was 0.15 g with a high percentage removal of CR (98.24%) and RhB (99.86%) dyes. The adsorption isotherm and kinetic studies were found to be favorable and feasible for assessing the adsorption of dyes with the Langmuir model and pseudo-second-order reaction, respectively. In addition, the AC showed reusability up to five cycles. The results showed that the synthesized AC was environmentally friendly and successfully removed dyes from wastewater.
Chemical Profiling of Pistacia lentiscus var. Chia Resin and Essential Oil: Ageing Markers and Antimicrobial Activity
Authors: Pachi Vasiliki K. , Mikropoulou Eleni V. , Dimou Sofia , Dionysopoulou Mariangela , Argyropoulou Aikaterini , Diallinas George , Halabalaki Maria
Published: February 2021

Chios Mastic Gum (CMG) and Chios Mastic Oil (CMO) are two unique products of the tree Pistacia lentiscus var. Chia, cultivated exclusively on the Greek island of Chios. In the present study, the method proposed by the European Pharmacopoeia for mastic identification was employed using HPTLC together with an in-house method. A GC-MS methodology was also developed for the chemical characterization of CMOs. α-Pinene and β-myrcene were found in abundance in the fresh oils; however, in the oil of the aged collection, oxygenated monoterpenes and benzenoids such as verbenone, pinocarveol, and α-campholenal were found at the highest rates. Additionally, the antimicrobial activity of Chios Mastic Gums (CMGs) with their respective Chios Mastic Oils (CMOs) was evaluated, with growth tests against the fungi Aspergillus nidulans, Aspergillus fumigatus, Candida albicans, Mucor circinelloides, and Rhizopus oryzae, and the bacteria Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis, with the samples exhibiting a moderate activity. To our knowledge, this is the first time that an HPTLC method is proposed for the analysis of mastic and its essential oil and that a standardized methodology is followed for the distillation of CMO with a parallel assessment of the ageing effect on the oil’s composition.
Sage (Salvia officinalis L.) Essential Oil as a Potential Replacement for Sodium Nitrite in Dry Fermented Sausages
Authors: Šojić Branislav , Tomović Vladimir , Savanović Jovo , Kocić-Tanackov Sunčica , Pavlić Branimir , Jokanović Marija , Milidrag Ardea , Martinović Aleksandra , Vujadinović Dragan , Vukić Milan
Published: February 2021

This study investigates the effects of sodium nitrite replacement by the sage essential oil (SEO), on the physico-chemical, microbiological and sensory quality of dry fermented sausages (DFS) during 225 days of storage. The SEO (0.00, 0.05 and 0.10 µL/g) was added in DFS batters formulated with different levels of pork back fat (15% and 25%) and sodium nitrite (0, 75 and 150 mg/kg). The inclusion of SEO had no negative impact on pH, color (instrumental and sensory) and texture parameters. Total plate counts were lower than 6 log CFU (colony forming units)/g in all samples throughout the storage. Furthermore, the addition of SEO at concentration of 0.05 µL/g provided acceptable TBARS (2-Thiobarbituric acid reactive substances) values (<0.3 mg MDA (malondialdehyde)/kg) in the samples produced with reduced levels of sodium nitrite (0 and 75 mg/kg) without negative alternations on sensory attributes of odor and flavor. Generally, our findings confirmed that the usage of SEO could be a good solution to produce healthier DFS with reduced levels of sodium nitrite.
Using Peptidomics and Machine Learning to Assess Effects of Drying Processes on the Peptide Profile within a Functional Ingredient
Authors: Chauhan Sweeny , O’Callaghan Sean , Wall Audrey , Pawlak Tomasz , Doyle Ben , Adelfio Alessandro , Trajkovic Sanja , Gaffney Mark , Khaldi Nora
Published: February 2021

Bioactive peptides are known to have many health benefits beyond nutrition; yet the peptide profile of high protein ingredients has been largely overlooked when considering the effects of different processing techniques. Therefore, to investigate whether drying conditions could affect the peptide profile and bioactivity within a functional ingredient, we examined the effects of spray (SD) and freeze (FD) drying on rice natural peptide network (NPN), a characterised functional ingredient sourced from the Oryza sativa proteome, which has previously been shown to effectively modulate circulating cytokines and improve physical performance in humans. In the manufacturing process, rice NPN was either FD or SD. Employing a peptidomic approach, we investigated the physicochemical characteristics of peptides common and unique to FD and SD preparations. We observed similar peptide profiles regarding peptide count, amino acid distribution, weight, charge, and hydrophobicity in each sample. Additionally, to evaluate the effects of drying processes on functionality, using machine learning, we examined constituent peptides with predicted anti-inflammatory activity within both groups and identified that the majority of anti-inflammatory peptides were common to both. Of note, key bioactive peptides validated within rice NPN were recorded in both SD and FD samples. The present study provides an important insight into the overall stability of the peptide profile and the use of machine learning in assessing predicted retention of bioactive peptides contributing to functionality during different types of processing.
Adaptation and Validation of a Short Acculturation Scale in a Multi-Ethnic Asian Population
Authors: Park Su Hyun , Abdin Edimansyah , Nan Luo , Subramaniam Mythily , Tan Linda Wei Lin , van Dam Rob M
Published: February 2021

The Short Acculturation Scale (SAS) has been widely used for assessing the level of the acculturation of migrants in Western countries. However, the validity of SAS for use in cosmopolitan settings without a single prevailing culture is unclear. We examined the validity and reliability of a version of the SAS adapted to a multi-ethnic Asian society. We used cross-sectional data from 12,610 Singaporean citizens and permanent residents, aged 21–75 years, of Chinese, Malay, and Indian ethnicity. Our version used 11 items, with 5 questions on language use, 3 on media use, and 3 on ethnic social relations, to measure acculturation. Our version of the SAS had good internal consistency. The three-factor CFA model had a good fit to our data. The results from the multiple group CFA supported metric invariance and partial scalar invariance across the three ethnic groups. The total score was positively correlated with generation in Singapore and the number of languages spoken. Among first generation immigrants, country of origin, but not the duration of residence was significantly associated with the acculturation score. Our three-factor version of the SAS is a reliable and valid tool for measuring acculturation in Singapore residents. These findings indicate that adapted SAS can be used to assess acculturation in multicultural settings.
The Study on Stress, Spirituality, and Health (SSSH): Psychometric Evaluation and Initial Validation of the SSSH Baseline Spirituality Survey
Authors: Warner Erica T. , Kent Blake Victor , Zhang Ying , Argentieri M. Austin , Rowatt Wade C. , Pargament Kenneth , Koenig Harold G. , Underwood Lynn , Cole Shelley A. , Daviglus Martha L. , Kanaya Alka M. , Palmer Julie R. , Huang Tianyi , Blais Mark A. , Shields Alexandra E.
Published: February 2021

This paper describes the development and initial psychometric testing of the baseline Spirituality Survey (SS-1) from the Study on Stress, Spirituality, and Health (SSSH). The SS-1 contains a mixture of items selected from validated existing scales and new items generated to measure important constructs not captured by existing instruments, and our purpose here was to establish the validity of new and existing measures in a racially/ethnically diverse sample. Psychometric properties of the SS-1 were evaluated using standard psychometric analyses in 4563 SSSH participants. Predictive validity of SS-1 scales was assessed in relation to the physical and mental health component scores from the Short-Form 12 Health Survey (SF-12). Scales exhibited adequate to strong psychometric properties and demonstrated construct and predictive validity. Overall, the correlational findings provided solid evidence that the SS-1 scales are associated with a wide range of relevant R/S attitudes, mental health, and to a lesser degree physical health.
A Qualitative Study on the Experience of Military Life and Ministry of Korean Christian Female Military Pastors
Authors: Yoo Jieun
Published: February 2021

The purpose of this study was to understand who female military pastors are in the Korean military and examine their military lives and ministry experiences through in-depth interviews and qualitative analysis. Six of the eight female military pastors currently working participated in this study. Seven major themes were identified: motivation to apply, role confusion and ambiguity, token women in the Korean military, perceived barriers or bias, equality experience, lack of network or mentor, and work and family balance. This study was helpful for obtaining a deeper understanding of female military pastors who exist as a minority in the Korean military. This study also provides basic information for the seminary education of future female military pastors and policies for female leaders working in the Korean military.
Periglacial Lake Origin Influences the Likelihood of Lake Drainage in Northern Alaska
Authors: Lara Mark Jason , Chipman Melissa Lynn
Published: February 2021

Nearly 25% of all lakes on earth are located at high latitudes. These lakes are formed by a combination of thermokarst, glacial, and geological processes. Evidence suggests that the origin of periglacial lake formation may be an important factor controlling the likelihood of lakes to drain. However, geospatial data regarding the spatial distribution of these dominant Arctic and subarctic lakes are limited or do not exist. Here, we use lake-specific morphological properties using the Arctic Digital Elevation Model (DEM) and Landsat imagery to develop a Thermokarst lake Settlement Index (TSI), which was used in combination with available geospatial datasets of glacier history and yedoma permafrost extent to classify Arctic and subarctic lakes into Thermokarst (non-yedoma), Yedoma, Glacial, and Maar lakes, respectively. This lake origin dataset was used to evaluate the influence of lake origin on drainage between 1985 and 2019 in northern Alaska. The lake origin map and lake drainage datasets were synthesized using five-year seamless Landsat ETM+ and OLI image composites. Nearly 35,000 lakes and their properties were characterized from Landsat mosaics using an object-based image analysis. Results indicate that the pattern of lake drainage varied by lake origin, and the proportion of lakes that completely drained (i.e., >60% area loss) between 1985 and 2019 in Thermokarst (non-yedoma), Yedoma, Glacial, and Maar lakes were 12.1, 9.5, 8.7, and 0.0%, respectively. The lakes most vulnerable to draining were small thermokarst (non-yedoma) lakes (12.7%) and large yedoma lakes (12.5%), while the most resilient were large and medium-sized glacial lakes (4.9 and 4.1%) and Maar lakes (0.0%). This analysis provides a simple remote sensing approach to estimate the spatial distribution of dominant lake origins across variable physiography and surficial geology, useful for discriminating between vulnerable versus resilient Arctic and subarctic lakes that are likely to change in warmer and wetter climates.