bluuebunny/crossref_metadata_2025 huggingface dataset:
The Suzuki-Miyaura reaction in the chemical transformations of vindoline.
Authors: Ruszkowska Joanna , Chrobak Robert , Zero Paweł , Maurin Jan K , Szawkało Joanna , Czarnocki Zbigniew
Published: November 2019

Vindoline and its analogues are important constituents of the Madagascan periwinkle Catharanthus roseus, and some of them are valuable chemotherapy drugs used in treatment for some types of cancer, including leukaemia, lymphoma, breast and lung cancer. The search for semi-synthetic congeners of natural substances is still an important task for organic chemistry. In this communication we report the synthesis of five new vindoline derivatives, 15-(2-methoxyphenyl)vindoline 11, 15-(3-methoxyphenyl)vindoline 12, 15-(2-nitrophenyl)vindoline 13, 15-(3-cyanophenyl)vindoline 15, and 15-(4-cyanophenyl)vindoline 16 using the Suzuki-Miyaura reaction as the key step. X-Ray analysis of compound 16 is also reported.
Synthesis of a quaternary polyprenyl ammonium salt.
Authors: Sizova Olga V , Utkina Natalia S , Kalinchuk Nadezhda A , Danilov Leonid L , Maltsev Sergey D
Published: November 2019

Reaction of primary C(55)-allylic alcohol moraprenol (WT(3)C(7-9)-OH, a polyprenol from mulberry leaves) with triethylamine in the presence of phosphorus oxychloride leads to a quaternary ammonium chloride with a good yield (72%) and high cis-stereoselectivity of the terminal isoprene unit. Cationic polyprenyl derivatives may be useful for transfection and immunological studies.
Multicellular behaviour and production of a wide variety of toxic substances support usage of Bacillus subtilis as a powerful biocontrol agent.
Authors: Nagórska Krzysztofa , Bikowski Mariusz , Obuchowski Michał
Published: November 2019

Intensive cultivation of plants in the monoculture field system in order to feed the continuously growing human population creates a need for their protection from the variety of natural competitors such as: bacteria, fungi, insects as well as other plants. The increase in the use of chemical substances in the 20th century has brought many effective solutions for the agriculture. However, it was extremely difficult to obtain a substance, which would be directed solely against a specific plant pathogen and would not be harmful for the environment. In the late 1900's scientists began trying to use natural antagonisms between resident soil organism to protect plants. This phenomenon was named biocontrol. Biological control of plants by microorganisms is a very promising alternative to an extended use of pesticides, which are often expensive and accumulate in plants or soil, having adverse effects on humans. Nonpathogenic soil bacteria living in association with roots of higher plants enhance their adaptive potential and, moreover, they can be beneficial for their growth. Here, we present the current status of the use of Bacillus subtilis in biocontrol. This prevalent inhabitant of soil is widely recognized as a powerful biocontrol agent. Naturally present in the immediate vicinity of plant roots, B. subtilis is able to maintain stable contact with higher plants and promote their growth. In addition, due to its broad host range, its ability to form endospores and produce different biologically active compounds with a broad spectrum of activity, B. subtilis as well as other Bacilli are potentially useful as biocontrol agents.
Engineered resistance against proteinases.
Authors: Milner Malgorzata , Chroboczek Jadwiga , Zagorski-Ostoja Wlodzimierz
Published: November 2019

Exogenous proteinase inhibitors are valuable and economically interesting protective biotechnological tools. We examined whether small proteinase inhibitors when fused to a selected target protein can protect the target from proteolytic degradation without simultaneously affecting the function and activity of the target domain. Two proteinase inhibitors were studied: a Kazal-type silk proteinase inhibitor (SPI2) from Galleria mellonella, and the Cucurbita maxima trypsin inhibitor I (CMTI I). Both inhibitors target serine proteinases, are small proteins with a compact structure stabilized by a network of disulfide bridges, and are expressed as free polypeptides in their natural surroundings. Four constructs were prepared: the gene for either of the inhibitors was ligated to the 5' end of the DNA encoding one or the other of two selected target proteins, the coat protein (CP) of Potato potyvirus Y or the Escherichia coli beta-glucuronidase (GUS). CMTI I fused to the target proteins strongly hampered their functions. Moreover, the inhibitory activity of CMTI I was retained only when it was fused to the CP. In contrast, when fused to SPI2, specific features and functions of both target proteins were retained and the inhibitory activity of SPI2 was fully preserved. Measuring proteolysis in the presence or absence of either inhibitor, we demonstrated that proteinase inhibitors can protect target proteins used either free or as a fusion domain. Interestingly, their inhibitory efficiency was superior to that of a commercial inhibitor of serine proteinases, AEBSF.
Fold recognition insights into function of herpes ICP4 protein.
Authors: Wyrwicz Lucjan S , Rychlewski Leszek
Published: November 2019

ICP4 is an important factor regulating the life cycle of HSV1. This conserved protein has several molecular functions, including activation of expression of viral late gene transcripts and inhibition of immediate early genes. Although ICP4 and its Alphaherpesvirinae homologs (eg.: IE62 of VZV) have been subjects of various molecular studies, a complete view of their molecular function is lacking. Here we present the results of fold recognition and molecular modelling of ICP4 functional domains. The performed state-of-the-art bioinformatic fold recognition analysis identified a dual helix-turn-helix motif as a binding module of repressor activities (so called region 2 domain). The mapping of distant homology identified that a segment responsible for activation of late gene promoters (region 4) exhibits folding of uracil DNA glycosylase (UDG), but seems to be a non-functional homolog of UDG. Potential implications of the results are discussed.
A new method to precipitate myosin V from rat brain soluble fraction.
Authors: Melo Hugo Christiano Soares , Coelho Milton Vieira
Published: November 2019

Myosin can be precipitated from soluble fraction under different assay conditions. This paper describes a new method for precipitating myosin V from rat brain soluble fraction. Brains were homogenized in 50 mM imidazole/HCl buffer, pH 8.0, containing 10 mM EDTA/EGTA, 250 mM sucrose, 1 mM DTT and 1 mM benzamidine, centrifuged at 45000 x g for 40 min and the supernatant was frozen at -20 degrees C. Forty-eight hours later, the supernatant was thawed, centrifuged at 45000 x g for 40 min and the precipitate was washed in 20 mM imidazole buffer pH 8.0. SDS/PAGE analysis showed four polypeptides in the precipitate: 205, 150, 57 and 43 kDa. The precipitate presented high Mg(2+)-ATPase activity, which co-purifies with p205. This polypeptide was recognized by a specific myosin V antibody and was proteolised by calpain, generating two stable polypeptides: p130 and p90. The Mg(2+)-ATPase activity was not stimulated by calcium in both the absence and presence of exogenous calmodulin and the K+/EDTA-ATPase activity represented 25% of the Mg(2+)-ATPase activity. In this work, myosin V from rat brain was precipitated by freezing the soluble fraction and was co-purificated with a 45 kDa polypeptide.
Elevated cyclin E level in human clear cell renal cell carcinoma: possible causes and consequences.
Authors: Nauman Alicja , Turowska Olga , Poplawski Piotr , Master Adam , Tanski Zbigniew , Puzianowska-Kuznicka Monika
Published: November 2019

The expression of cyclin E gene (CCNE) in relation to the expression of its major regulatory protein, E2F1, was examined in clear cell renal cell carcinomas (ccRCC). We show that the overexpression of E2F1 is accompanied by the significant increase of the mean amounts of cyclin E mRNA, as well as of total cyclin E protein and its low molecular weight forms in cancer tissues as compared to peritumoral controls. A significant increase of the mean amount of total cyclin E was found in peritumoral tissues compared to cancer-free kidneys, suggesting that cancer cells might secrete factors having a profound influence on the metabolism of neighbouring tissues. A significant, positive correlations between E2F1 protein and total cyclin E mRNA, as well as between E2F1 protein and full length cyclin E protein were found in cancer-free kidneys and in peritumoral tissues, but not in ccRCCs. The overexpression of cyclin E positively correlated with the decreasing degree of tumor differentiation, implicating a role for cyclin E in the promotion of tumorigenesis.
The role of actin and microtubule networks in plasmid DNA intracellular trafficking.
Authors: Ondrej Vladan , Lukásová Emilie , Falk Martin , Kozubek Stanislav
Published: November 2019

This work is focused on the function of the microtubule and actin networks in plasmid DNA transport during liposomal transfection. We observed strong binding of plasmid DNA-lipid complexes (lipoplexes) to both networks and directional long-range motion of these lipoplexes along the microtubules. Disruption of either of these networks led to the cessation of plasmid transport to the nucleus, a decreased mobility of plasmids, and accumulation of plasmid DNA in large aggregates at the cell periphery. Our findings show an indispensable but different role of both types of cytoskeleton, actin and microtubular, in the processes of gene delivery.
A freeze-thaw method for disintegration of Escherichia coli cells producing T7 lysozyme used in pBAD expression systems.
Authors: Wanarska Marta , Hildebrandt Piotr , Kur Józef
Published: November 2019

The pLysN plasmid containing the T7 lysozyme gene under control of the lac promoter was constructed to facilitate cell disintegration after expression of recombinant proteins in arabinose-induced expression systems. The usefulness of this plasmid was tested in Escherichia coli TOP10 and E. coli LMG194 cells carrying pBADMHADgeSSB plasmid containing Deinococcus geothermalis SSB protein gene under control of the araBAD promoter. The results showed that low-level expression of T7 lysozyme did not interfere with the target SSB protein production, and that the freezing-thawing treatment was sufficient for disruption of the E. coli cells producing low amounts of T7 lysozyme.
Structural basis of the interspecies interaction between the chaperone DnaK(Hsp70) and the co-chaperone GrpE of archaea and bacteria.
Authors: Zmijewski Michał A , Skórko-Glonek Joanna , Tanfani Fabio , Banecki Bogdan , Kotlarz Agnieszka , Macario Alberto J L , Lipińska Barbara
Published: November 2019

Hsp70s are chaperone proteins that are conserved in evolution and present in all prokaryotic and eukaryotic organisms. In the archaea, which form a distinct kingdom, the Hsp70 chaperones have been found in some species only, including Methanosarcina mazei. Both the bacterial and archaeal Hsp70(DnaK) chaperones cooperate with a GrpE co-chaperone which stimulates the ATPase activity of the DnaK protein. It is currently believed that the archaeal Hsp70 system was obtained by the lateral transfer of chaperone genes from bacteria. Our previous finding that the DnaK and GrpE proteins of M. mazei can functionally cooperate with the Escherichia coli GrpE and DnaK supported this hypothesis. However, the cooperation was surprising, considering the very low identity of the GrpE proteins (26%) and the relatively low identity of the DnaK proteins (56%). The aim of this work was to investigate the molecular basis of the observed interspecies chaperone interaction. Infrared resolution-enhanced spectra of the M. mazei and E. coli DnaK proteins were almost identical, indicating high similarity of their secondary structures, however, some small differences in band position and in the intensity of amide I' band components were observed and discussed. Profiles of thermal denaturation of both proteins were similar, although they indicated a higher thermostability of the M. mazei DnaK compared to the E. coli DnaK. Electrophoresis under non-denaturing conditions demonstrated that purified DnaK and GrpE of E. coli and M. mazei formed mixed complexes. Protein modeling revealed high similarity of the 3-dimensional structures of the archaeal and bacterial DnaK and GrpE proteins.
Calcium- and proton-dependent relocation of annexin A6 in Jurkat T cells stimulated for interleukin-2 secretion.
Authors: Podszywalow-Bartnicka Paulina , Strzelecka-Kiliszek Agnieszka , Bandorowicz-Pikula Joanna , Pikula Slawomir
Published: November 2019

Annexin A6 (AnxA6) is a Ca(2+)-dependent membrane-binding protein involved in vesicular traffic. The likely participation of AnxA6 in the response of lymphocytes to Ca(2+) signals has not been investigated yet. The present study focuses on intracellular relocation of AnxA6 in human Jurkat T lymphoblasts upon stimulation followed by transient increase of intracellular [Ca(2+)] and exocytosis of interleukin-2 (IL-2). Stimulation of the cells under different experimental conditions (by lowering pH and/or by rising extracellular [Ca(2+)] in the presence of ionomycin) induced time-dependent transients of intracellular [Ca(2+)] and concomitant changes in AnxA6 intracellular localization and in IL-2 secretion, with only minor effects on cell viability and apoptosis. In resting conditions (in the presence of EGTA or with no ionophore) AnxA6 was localized uniformly in the cytosol, whereas it translocated to vesicular structures beneath the plasma membrane within 5 min following stimulation of Jurkat T cells and rise of intracellular [Ca(2+)] at pH 7.4. Lowering the extracellular pH value from 7.4 to 6.0 significantly enhanced this process. AnxA6 changed its location from the cytosol to the secretory granules and early endosomes which seem to represent membranous targets for annexin. In conclusion, AnxA6 is sensitive to variations in intracellular [Ca(2+)] upon stimulation of Jurkat T cells, as manifested by a switch in its intracellular localization from the cytosol to vesicular structures located in close proximity to the plasma membrane, suggestive of participation of AnxA6 in calcium- and proton-dependent secretion of cytokines by lymphocytes.
pVAX1 plasmid vector-mediated gene transfer of soluble TRAIL suppresses human hepatocellular carcinoma growth in nude mice.
Authors: Zhang Yan , Ma Cun Hong , Liu Hua , Zhang Xiu Mei , Sun Wen Sheng
Published: November 2019

The extracellular domain of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) may function as a soluble cytokine to selectively kill various cancer cells without toxicity to most normal cells. We used a high-biosafety plasmid pVAX1 as a vector and constructed a recombinant plasmid expressing the extracellular domain (95-281 aa) of human TRAIL fused with signal peptides of human IgGgamma, designated as pVAX-sT. Transduction of human BEL7402 liver cancer cells with pVAX-sT led to high levels of sTRAIL protein in the cell culture media and induced apoptosis. The therapeutic potential of pVAX-sT was then evaluated in the BEL7402 transplanted naked mouse model. Subsequent intratumoral administration of naked pVAX-sT resulted in the expression of soluble TRAIL in the sera and the tumor site, as well as effective suppression of tumor growth, with no toxicity to liver. In conclusion, the successful inhibition of liver cancer growth and the absence of detectable toxicity suggest that pVAX-sT could be useful in the gene therapy of liver cancer.
Plasma membrane homing of tissue nonspecific alkaline phosphatase under the influence of 3-hydrogenkwadaphnin, an antiproliferative agent from Dendrostellera lessertii.
Authors: Sadeghirizi Akram , Yazdanparast Razieh
Published: November 2019

Several mammalian enzymes are anchored to the outer surface of the plasma membrane by a covalently attached glycosylphosphatidylinositol (GPI) structure. These include acetylcholinesterase, alkaline phosphatase (AP) and 5'-nucleotidase among other enzymes. Recently, it has been reported that these membrane enzymes can be released into the serum by the GPI-dependent phospholipase D under various medical disturbances such as cancer and/or by chemical and physical manipulation of the biological systems. Treatment of MCF-7 cells with two consecutive effective concentrations of 3-hydrogenkwadaphnin (3-HK, 3 nM) for 48 h enhanced membrane AP activity by almost 330% along with a 40% reduction in the AP activity of the cell culture medium. In addition, our data indicate that 3-HK is capable of inducing mainly the tissue-nonspecific alkaline phosphatase (TNAP) isoenzyme, along with enhancing its thermostability. These findings, besides establishing a correlation between the antiproliferative activity of 3-HK and the extent of plasma membrane AP activity, might assist in the development of new diagnostic tools for following cancer medical treatments.
Nuclear localization and binding affinity of STAT5b for the alpha(2)-macroglobulin gene promoter during rat liver development and the acute-phase response.
Authors: Mihailović Mirjana , Dinić Svetlana , Bogojević Desanka , Ivanović-Matić Svetlana , Uskoković Aleksandra , Arambasić Jelena , Grigorov Ilijana , Grdović Nevena , Vidaković Melita , Martinović Vesna , Petrović Miodrag , Poznanović Goran
Published: November 2019

Expression of the rat alpha(2)-macroglobulin (MG) gene undergoes dynamic changes throughout an individual's life and during the acute-phase (AP) response. Details of the participation of the STAT family of transcription factors in its control remain incompletely understood. Here we examined the involvement of STAT5b in MG gene expression during development and the AP response. Immuno-blot analysis revealed the highest nuclear level of STAT5b in the fetus and during postnatal development, whereas in the adult it decreased. Stimulation of MG expression during the AP response was accompanied by a decrease in STAT5b. Examination of STAT5b localization revealed that the relative concentrations of STAT5b were higher in the nuclear matrix than in the nuclear extract. Affinity chromatography with the extended promoter region of the MG gene (-825/+12), followed by immuno-blot analysis, revealed dynamic changes in STAT5b binding. The highest concentration of the promoter-binding form of STAT5b was observed in the fetus. As postnatal development progressed, the level of promoter-bound STAT5b decreased and in the adult liver it was the lowest. Stimulation of MG gene expression during the AP response in both the fetus and adult was accompanied by significantly decreased STAT5b binding to the MG promoter. The AP response was accompanied by lower levels of STAT5b serine and tyrosine phosphorylation in both fetus and adult. In the nuclear matrix derived from adult tissues, tyrosine phosphorylated species were completely absent. We conclude that developmental-stage differences in the mechanisms that determine STAT5b nuclear localization contribute to its activity in vivo.
FGF binding by extracellular matrix components of Wharton's jelly.
Authors: Malkowski Andrzej , Sobolewski Krzysztof , Jaworski Stefan , Bankowski Edward
Published: November 2019

Our earlier paper has reported that Wharton's jelly is a reservoir of several peptide growth factors, including acidic and basic fibroblast growth factors (aFGF and bFGF, respectively). Both can be extracted by buffered salts solutions in the form of high molecular mass complexes, probably with a component(s) of the extracellular matrix. Both aFGF and bFGF from such extracts hardly penetrate 10% polyacrylamide gels during electrophoresis. Pre-treatment of Wharton's jelly with hyaluronidase slightly increased the extractability of aFGF, but did not affect the extractability of bFGF. In contrast, the pre-treatment of tissue homogenate with bacterial collagenase (2000 U/ml, 37 degrees C, 18 h) increased the extractability of bFGF. The presence of beta-mercaptoethanol in the extracting solutions increased the extractability of both FGFs, but did not release FGFs in their free form, despite reducing the molecular mass of the FGF-containing complexes. We conclude that both aFGF and bFGF are bound through disulphide bonds to a protein component of Wharton's jelly. We propose that ground substance composed mainly of collagen fibrils and hyaluronate molecules, which surrounds the cells of Wharton's jelly, prevents the access of the extracting solution to aFGF and bFGF. Although hyaluronate and collagen do not bind aFGF or bFGF directly, they may constitute a barrier which prevents the dispersion of FGFs in Wharton's jelly. Thus, the high concentration of FGFs around the cells of Wharton's jelly may facilitate the interaction of these factors with membrane receptors, thereby resulting in stimulation of cell division and differentiation, as well as of the synthesis of extracellular matrix components.
Kinetic intermediates of unfolding of dimeric prostatic phosphatase.
Authors: Kuciel Radosława , Mazurkiewicz Aleksandra , Dudzik Paulina
Published: November 2019

Kinetics of guanidine hydrochloride (GdnHCl)-induced unfolding of human prostatic acid phosphatase (hPAP), a homodimer of 50 kDa subunit molecular mass was investigated with enzyme activity measurements, capacity for binding an external hydrophobic probe, 1-anilinonaphtalene-8-sulfonate (ANS), accessibility of thiols to reaction with 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and 2-(4'-maleimidylanilino)naphthalene-6-sulfonate (MIANS) and ability to bind Congo red dye. Kinetic analysis was performed to describe a possible mechanism of hPAP unfolding and dissociation that leads to generation of an inactive monomeric intermediate that resembles, in solution of 1.25 M GdnHCl pH 7.5, at 20 degrees C, in equilibrium, a molten globule state. The reaction of hPAP inactivation in 1.25 M GdnHCl followed first order kinetics with the reaction rate constant 0.0715 +/- 0.0024 min(-1) . The rate constants of similar range were found for the pseudo-first-order reactions of ANS and Congo red binding: 0.0366 +/- 0.0018 min(-1) and 0.0409 +/- 0.0052 min(-1), respectively. Free thiol groups, inaccessible in the native protein, were gradually becoming, with the progress of unfolding, exposed for the reactions with DTNB and MIANS, with the pseudo-first-order reaction rate constants 0.327 +/- 0.014 min(-1) and 0.216 +/- 0.010 min(-1), respectively. The data indicated that in the course of hPAP denaturation exposure of thiol groups to reagents took place faster than the enzyme inactivation and exposure of the protein hydrophobic surface. This suggested the existence of a catalytically active, partially unfolded, but probably dimeric kinetic intermediate in the process of hPAP unfolding. On the other hand, the protein inactivation was accompanied by exposure of a hydrophobic, ANS-binding surface, and with an increased capacity to bind Congo red. Together with previous studies these results suggest that the stability of the catalytically active conformation of the enzyme depends mainly on the dimeric structure of the native hPAP.
SFRP1 inhibited the epithelial ovarian cancer through inhibiting Wnt/β-catenin signaling
Authors: Zhang Hao , Sun Duohe , Qiu Jianping , Yao Liangqing
Published: November 2019

Background: Epithelial ovarian cancer is the most malignant gynecologic neoplasm accounting for 90% of the ovarian cancer patients. Objective: Researchers proved that epigenetic alterations could disrupt gene expression as often as genetic alterations. Secreted frizzed related protein (SFRP1), a Wnt antagonist, exerts a significant effect on ovarian cancer. The aim of this research was to investigate the effects and the mechanism of action of SFRP1 on epithelial ovarian cancer. Methods: Clinical specimens (including fallopian tubes epithelium from 60 epithelial ovarian cancer patients’ and 20 healthy subjects who were undergoing surgical treatments), transgenic mice (overexpressing SFRP1 gene), and 4 epithelial ovarian cancer cell lines (including OVCAR4, SKOV3, COV644, TOV21G) were used in this study. Overexpression of SFRP1 in cells was carried out on OVCAR4 cells by transfection using Lipofectamine 2000. Gene transcription was analyzed by qRT-PCR. The methylation of SFRP1 gene was quantified by methylation-specific PCR. The level of protein expression was measured by Western blot or immunohistochemistry analysis. Cell proliferation was analyzed by CCK8 methods. The ability of cell migration and invasion were measured by wound healing assay and transwell assay. Results: Abnormal expression level and hypermethylation status of SFRP1 were found in clinical epithelial ovarian cancer samples and cell lines. We observed that SFRP1 knockdown could promote proliferation, migration and invasion abilities of epithelial ovarian cancer cells. Additionally, we discovered a potential inhibitory effect of SFRP1 on Wnt/β-catenin signaling pathway in epithelial ovarian cancer cells. Furthermore, the anti-tumor effect of SFRP1 was tested in SFRP1 transgenic mice. Conclusion: SFRP1 inhibited epithelial ovarian cancer through inhibiting Wnt/β-catenin pathway, suggesting that SFRP1 could be considered as a potential therapeutic target in epithelial ovarian cancer.
Eosinophilic Gastroenteritis and Familial Mediterranean Fever in a Child With MEFV Gene Mutation
Authors: Mehrabani Sanaz , Pornasrollah Mohammad , Moslemi Leila
Published: November 2019

Eosinophilic gastroenteritis (EG) is a rare inflammatory disorder affecting both children and adults. The exact etiology of the disease is not clear. A child presented with episodic generalized abdominal pain since a year ago without fever at first. After endoscopic and colonoscopic examinations, histopathological examination showed an increased number of eosinophils and diagnosis of EG was made. After elimination of dairy products from his regimen, abdominal pain attacks was reduced, but he got a fever. Familial Mediterranean Fever (FMF) diagnosis was made by genetic evaluation which showed MEV gene mutation. Symptoms were resolved with the treatment of colchicine which confirmed FMF diagnosis.
© 2019 Tehran University of Medical Sciences. All rights reserved.
Acta Med Iran 2019;57(5):328-331.
Non Salicylate-Associated Reye Syndrome: A Case Report
Authors: Azizi Arsalan , Sedaghattalab Moslem
Published: November 2019

Reye's syndrome (RS) is a rare disease, usually associated with consumption of salicylates during viral illness. In 1965, the first case of association between RS and salicylates was described in United Kingdom (UK). The incidence of RS decreased dramatically after warnings of UK and US health agencies against using aspirin in children. Patients with RS presented with neurologic compromise, cerebral edema, acute hepatitis, and liver failure-especially in the children.  In this paper, a four-month-old boy with diagnosis of RS was described, who presented with malaise, cyanosis and decreased level of consciousness, but the history of salicylates consumption was negative for him and his mother.
© 2019 Tehran University of Medical Sciences. All rights reserved.
Acta Med Iran 2019;57(5):332-334.
Promoter Hypermethylation of the Eyes Absent 4 Gene Is a Tumor-Specific Epigenetic Biomarker in Iranian Colorectal Cancer Patients
Authors: Barati Bagerabad Matineh , Tavakolian Sahar , Abbaszadegan Mohammad Reza , Kerachian Mohammad Amin
Published: November 2019

Abstract
Planning Around Reserves: Probing the Inclusion of First Nations in Saskatchewan's Watershed Planning Framework
Authors: Baijius Warrick , Patrick Robert J.
Published: November 2019

Watershed-based planning in Saskatchewan began in earnest after 2006 under the auspices of the Saskatchewan Watershed Authority. Within a decade, a dozen watershed plans were produced following a planning framework that included technical and watershed resident committees. First Nation communities, or "reserves," exist within these watershed areas. This article probes the inclusion of First Nations in those plans. Using document analysis and keyword search, our analysis explores any spatial relationship that may exist between First Nation inclusion and the amount of reserve land in a watershed. The results of this research show that First Nation inclusion is limited in watershed planning in Saskatchewan. We see opportunity for more effective watershed planning through greater collaboration with First Nations.
Análisis de costo-efectividad del uso de calcio más ácido linoleico para la prevención de la hipertensión inducida por el embarazo en mujeres con riesgo en Colombia
Authors: Romero-Prada Martín , Alvis-Guzmán Nelson , Karpf-Benavides Elizabeth
Published: November 2019

Objetivo: analizar la costo-efectividad del uso de calcio-ácido linoleico y control prenatal frente a calcio y control prenatal para prevenir hipertensión inducida por el embarazo (HIE) en mujeres con riesgo en Colombia, desde la perspectiva del tercero pagador.Materiales y métodos: en un árbol de decisiones se simuló la historia natural de la enfermedad. Las medidas de resultado fueron la mortalidad materna y perinatal evitadas. Los costos de los medicamentos fueron los promedios del mercado (2010). Los costos directos de atención se tomaron de aseguradores y de registros individuales de las prestaciones de salud.Resultados: la razón de costo-efectividad incremental fue menor en el brazo tratado con calcio-ácido linoleico que en el tratado con calcio para ambos desenlaces ($723.788 por año de vidas salvadas en madres y $103.741 por año de vidas salvadas en hijos frente a $4.709.708 y $2.240.294 para el brazo de solo calcio respectivamente). La comparación entre calcio-ácido linoleico y calcio revela dominancia del primero en términos de costo-efectividad incremental para ambos desenlaces, dado que es más efectivo (0,44 años de vida salvados en madres y 11,84 años de vida salvados en hijos) y menos costoso ($6.676.952) para el caso base. La razón incremental de costo-efectividad entre calcio-ácido linoleico y control prenatal sería a favor del primero tanto en el desenlace materno como perinatal.Conclusión: para Colombia, el uso de calcio-ácido linoleico es el mejor tratamiento en mujeres con riesgo de HIE, frente a calcio o control prenatal, tomando como indicador las muertes maternas y perinatales evitadas.
Pertinencia del tamizaje genético en donantes de gametos
Authors: Serrano-Serrano Claudia J.
Published: November 2019

Introducción: una preocupación importante para las parejas sometidas a tratamientos de reproducción asistida con gametos donados es la posibilidad de adquirir enfermedades infecciosas o la transmisión de enfermedades genéticas a la descendencia. Existen reportes, basados en la legislación de cada país y en la experiencia de cada centro, que hacen referencia al tipo de evaluación genética, infecciosa y psicológica que deben tener los donantes de gametos. En Colombia no existe una ley específica sobre las técnicas de reproducción asistida y la donación de gametos. Tampoco existe claridad acerca de si los donantes deberían ser evaluados genéticamente y con qué tipo de pruebas.Objetivo: el objetivo de este documento es hacer una reflexión sobre la pertinencia de evaluar genéticamente a los donantes de gametos a partir de la caracterización de las principales condiciones genéticas prevalentes en nuestra población, la alteración genética de base y el método actual de diagnóstico genético.Conclusión: se concluye que es necesario desarrollar un protocolo claro a nivel nacional y en los centros de reproducción asistida a cerca de la evaluación genética en los pacientes donantes de gametos.
Principios básicos de la Administración imperial romana
Authors: Quintana Orive Elena
Published: November 2019

El artículo examina los principios básicos de la organización de la Administración imperial romana, basada en la formación de una burocracia fuertemente jerarquizada. Se estudian también los requisitos de los candidatos para acceder a la función pública y se da una visión de conjunto sobre la responsabilidad en la que podían incurrir los magistrados y funcionarios en el ejercicio de su cargo.
Desafíos de la función pública en Chile y medidas para la modernización y buena gestión de las relaciones laborales dentro de la Administración del Estado
Authors: Gómez González Rosa Fernanda
Published: November 2019

La presente investigación tiene por objeto examinar la situación actual de la función pública en Chile, explicando los principales aspectos de conflicto que han tenido lugar, fundamentalmente por la obsolescencia del sistema de carrera de funcionarios, así como por la llamada laboralización del empleo público. Luego de identificados los aspectos críticos ligados a estas evoluciones, se analizan una serie de medidas que deberían ser adoptadas para el fortalecimiento del sistema de carrera y que permitirían alcanzar la modernización del mismo y una buena gestión de las relaciones laborales al interior de la Administración del Estado.
Las medidas correctoras del abuso de la temporalidad de los empleados públicos en España
Authors: Sánchez García Alfonso
Published: November 2019

El presente artículo analiza las medidas adoptadas por el ordenamiento jurídico español a efectos de prevenir y sancionar el extendido e inapropiado uso del empleo público temporal para la cobertura de puestos de trabajo de carácter permanente y el consiguiente fraude a la obligación legal de recurrir, como regla general, al empleo indefinido. Se examina una situación de gran actualidad en la que, a las últimas sentencias del Tribunal Supremo, se añaden inminentes pronunciamientos del Tribunal de Justicia de la Unión Europea, que podrían obligar a un cambio jurisprudencial en los fallos nacionales.
Las consecuencias impositivas de la declaración judicial del vínculo realidad en el contrato de prestación de servicios
Authors: Sanjuanelo Ortiz José
Published: November 2019

Si el juez administrativo ha construido una jurisprudencia garantista en cuanto al reconocimiento de prestaciones en favor del particular que logra probar que su vinculación contractual de servicios con el Estado se trató en la realidad de una relación subordinada de trabajo, la presente investigación enseña cómo estas decisiones pueden tornarse injustas cuando no consideran las cargas tributarias del aparente contratista. Este escrito busca enseñar que, en el evento en que es declarado el llamado contrato realidad, se debe entender que los tributos pagados por el contratista fueron cobrados con base en una causa inexistente o sin hecho generador: el desvirtuado contrato de prestación de servicios.
En defensa de la facultad de libre nombramiento y remoción: el camino de su insuficiente delimitación conceptual conduce a su cuestionable restricción
Authors: Marín Hernández Hugo Alberto
Published: November 2019

La categoría de los empleos de libre nombramiento y remoción pareciera haberse convertido en una gran incomprendida en el sistema jurídico colombiano. Ello obedece (1) a cierto déficit o confusión en punto de su justificación y delimitación conceptual, justificación que debe pasar por la necesidad de explicar la facultad de libre nombramiento y remoción como una modalidad de ejercicio de función política, más que como una especie de la función estrictamente administrativa. La inconsistencia dogmática que comporta el desconocimiento del carácter político de la citada potestad se traduce (2) en la inapropiada formulación de exigencias, como el reclamar que las decisiones de retiro del servicio se apoyen en la utilización del principio de proporcionalidad o el exigir que se deje constancia de los motivos de la desvinculación en la hoja de vida del servidor público. Esto, a pesar de que el acto de remoción del empleo no precise de motivación; y lo más grave aún, (3) a que se enerve hasta casi inutilizar la facultad mencionada. Esto podría tener un impacto nocivo para la buena marcha de las entidades del Estado, por la vía de entender aplicables a los empleos de libre nombramiento y remoción los supuestos de estabilidad laboral reforzada o de retén social que la normatividad vigente y sus desarrollos jurisprudenciales actualmente reconocen.
Total diz artroplastisinde insizyon kapatılırken diz pozisyonunun, postoperatif ağrı ve fonksiyona etkisi
Authors: PAMUK Çağdaş
Published: November 2019

Amaç

Literatürde total diz artroplastisi
(TDA) sırasında inzisyonun hangi pozisyonda kapatılması gerektiği konusunda
fikir birliği yoktur. İnsizyon bölgesindeki gerginlik, hematom oluşması aynı
zamanda ekstensör mekanizmanın uyumsuz kapatılmasının erken dönemde kötü
fonksiyonel sonuçlar doğurabileceği bildirilmiştir. Bu çalışmada TDA sırasında
dizin tam fleksiyon pozisyonunda insizyonun kapatılması ile tam ekstansiyon
pozisyonda kapatılmasının, postoperatif ağrı ve fonksiyona olan etkisini
araştırmayı amaçladık.

Gereç
ve Yöntemler

İleri derece gonartroz nedeniyle TDA
operasyonu planlanan 61 hasta çalışmaya dahil edildi. TDA sırasından insizyonu
diz maksimum fleksiyon pozisyonundayken sütüre edilen 30 hasta Grup 1 olarak,
tam ekstansiyon pozisyonunda sütüre edilen 31 hasta Grup 2 olarak belirlendi.
Gruplar arasında (1) hastaların demografik özellikleri (yaş, kilo, beden kitle
indeksi (BKİ)) (2) preop, postop 1. hafta, postop 2. hafta eklem hareket
açıklığı değerleri ve ağrı skorları karşılaştırıldı.

Bulgular

Gruplar arasında ağrı değerleri
açısından preoperatif dönemde anlamlı bir fark yoktur. Postop 1. haftada eklem
hareket açıklığı ve ağrı skorları Grup 1’de anlamlı oranda düşüktür
(p<0,001). 6. hafta incelendiğinde gruplar arasında anlamlı bir fark
görülmemektedir.

Sonuç

TDA da insizyonun hangi pozisyonda
kapatılacağı konusunda fikir birliği yoktur. Ancak literatürde ve bu çalışmada
görüldüğü gibi fleksiyon pozisyonunda gerçekleştirilen onarımların fonksiyonel
sonuçlarının erken dönemde daha iyi olduğu düşüncesindeyiz.
XXS patient with an XXL aorta
Authors: AVCI DEMİR Fulya , ERKAN Arda , PAPACHRİSTİDİS Alexandros , THEODOROPOULOS Konstantinos
Published: November 2019

An
85-year-old woman with small body size was referred to our cardiology clinic
due to a longstanding nonspecific abdominal pain. A routine transthoracic
echocardiogram revealed a massive ascending aorta aneurysm. A chest CT
demonstrated a huge aneurysm of thoracic and abdominal aorta.
Sistem Prediksi Jumlah Permintaan Produk Darah Menggunakan Metode Least Square Regression Line (Studi Kasus : Utd Pmi Kabupaten Jombang)
Authors: Nasyika Dzurrotun , Slamin Slamin , Pandunata Priza
Published: November 2019

Blood Transfusion Unit (UTD) as the provider of blood supply is required to fulfill blood demand. However in reality, the blood stock does not always meet the demand for blood. The amount of blood group stock in UTD depends on blood donors who voluntarily donate blood. Red blood cells only have a life span of 35 days since they were donated, if they have passed that time the blood cannot be used for transfusion. Based on these problems, the authors applied the Lesat Square Regression Line method to predict the number of requests for blood products in the coming month. The calculation results obtained prediction of demand with the smallest MAPE value that is blood product of  PRC blood type A with a value of 14.40%. The biggest MAPE value obtained is 180.66% for WB blood type AB. Factors or parameters that affect the amount of blood product demand, that is the environment, disease outbreaks and differences in body resistance of each blood group. In addition, the types of blood products also have a number of different requests depending on the level of blood needs for health.
Sistem Informasi Kartu Pegawai Elektronik (SI-KPE) Berbasis Web dan Mobile KPE Berbasis Android Dengan Menggunakan Metode AES-128
Authors: Nurdiansyah Yanuar , Slamin Slamin , Priaditama Juniar
Published: November 2019

East Java regional development Bank, known as the Bank of East Java was founded on august 17th 1951 in Surabaya. Bank of East Java has a lot of products and services for both civil society or non- civilservants.Oneofthemistheproduct elektronicservisservantcard(KPE).Datamanagementservices servants electronic card is very simple, make inefficient in terms of time and effort because the input file and file storage are still using manual system, as well as to disseminate announcements or events newest still using posters and other print media. File and information would be usefull if it is delivered to the user with an interest in the proper way. Currently, almost all file and information submitted throught the internet network. Security and confidentiality of file submitted via the internet is vulnerable to file theft by unauthorized parties. One way to maitain the security and confidentiality of such file is by using cryptographic methods. There are many cryptographic algorithms that are used to secure the file. One of which is an Algorithm Advanced Encryption Standard (AES). AES algorithm used in the reseacrh, namely AES-128 algorithm to encode digital files. So that the information contained in the file become more secure after converted into the file encrypt because the information can only be read by the party entitled. Employee card elektorinic information system (SI-KPE) and android based Mobile KPE using AES-128 method is a solution for Bank of East Java Jember branch
OPTIMASI PERSEDIAAN MATERIAL TRANSFORMATOR MENGGUNAKAN METODE JARINGAN SYARAF TIRUAN DAN ANT COLONY OPTIMIZATION DI PT. PLN (PERSERO) AREA JEMBER
Authors: Herdatullah Rizki , Bukhori Syaiful , Retnani Windi Eka Yulia
Published: November 2019

Optimization comes from basic words optimal which mean the best, highest, most beneficial, make the best, and do optimizing (make the best, highest, etc.). Forecasting is an attempt to predict the future. Prediction can be done by studying the pattern of historical data to find a model that can show future data. This methoed is called time series data forecasting. One of many algorithm that can builds model from historical data is Artificial Neural Networks (ANN). The algoritm mimics the human neuron system so that is can solve non-linear problems, such as the forecasting of transformator demand.

In the process of modeling, ANN will always update the connection weights to find the optimum weights. In this final project ANN will be trained by Ant Colony Optimization (ACO). Based on the results can be seen that ANN with ACO as learning methods can predict transformator demand with good result.
SEGMENTASI DAN PERHITUNGAN SEL DARAH PUTIH MENGGUNAKAN OPERASI MORFOLOGI DAN TRANSFORMASI WATERSHED
Authors: Alham Dwi Syamsuifin , Herumurti Darlis
Published: November 2019

Perhitungan sel darah putih terkait dengan sistem kekebalan tubuh manusia karena bisa memberikan informasi tentang kondisi tubuh manusia seperti diagnosis penyakit, salah satunya leukimia. Penyakit ini ditandai dengan produksi sel darah putih berlebih yang menyebabkan fungsi normal darah menjadi terganggu dan dapat menyebabkan kematian. Untuk itu diperlukan deteksi dini penyakit ini, yang salah satunya dengan menganalisis populasi atau menghitung jumlah sel darah putih pada citra mikroskopis sel darah. Perlu dilakukan metode segmentasi yang tepat agar hasil perhitungan sel dapat maksimal. Metode Watershed merupakan metode yang dapat memisahkan objek yang saling berhimpit, tetapi memiliki kelemahan jika dalam suatu citra terdapat noise atau distribusi intensitas yang tidak merata. Oleh karena itu, untuk mengatasi masalah tersebut, maka ditambahkan operasi morfologi di awal untuk mengatasi adanya obyek yang tidak termasuk sel darah putih. Tahap terakhir adalah menghitung sel darah putih menggunakan standar objek yang dianggap sel darah putih normal berdasarkan ukuran roundness obyek. Pengujian dilakukan dengan menggunakan 35 citra dengan menggunakan nilai SE = 20 pada operasi morfologi opening. Hasilnya, akurasi perhitungan jumlah sel antara sistem dengan manual (ground truth) sebesar 97.67%.
Increased Use Value of Coffee Bean Skin Waste as Liquid Fertilizer in Sukorambi Jember Area
Authors: Faizin Nur
Published: November 2019

The Faculty of Engineering's community service program, University of Jember, with a partner, the Sumber Kembang Kelompok Tani (Farmer Group), aims to overcome partner problems, about the accumulation of coffee bean skin waste that is not utilized. So far, the Sumber Kembang Kelompok Tani (Farmer Group) has not utilized coffee waste properly. The waste of the coffee bean skin will be processed into liquid fertilizer. The community service team will act as a facilitator and instructor in training or mentoring, which includes: 1. Explanation of the use and method of making liquid fertilizer from coffee bean skin, 2. Training related to the use of tools and processes for making liquid fertilizer, 3. Monitoring activities after socialization and training.  The results of this training and service are that farmers have the ability to make liquid organic fertilizer from coffee bean skin waste expected to increase partners' understanding in processing coffee bean skin waste into liquid fertilizer and adding economic value from coffee bean skin waste so that it has a higher selling value.
Coffee Processed Product Innovation in Kampung Pelita Bondowoso
Authors: Wildana Dina Tsalist , Furqoni Laili
Published: November 2019

Bondowoso has been known as a city of tape. This district also has the potential of coffee plantations that have produced products until it is known as the Republic of Coffee. The purpose of this activity is to increase public knowledge about various kinds of processed coffee, provide value added products, and attract consumers to come to visit the region. The method used is trial and error and utilizes the University of Jember Real Lecture Program where the target area is Sub-District Tamansari, Bondowoso District, Bondowoso Regency. The results of the activities include the emergence of innovations in processed coffee products such as: coffee dawet, coffee marrow porridge, Cilok Kopi, coffee fried rice and coffee ladrang. This activity was supported by PKK ladies and received support from Blue Gas. Such innovation activities are quite interesting for the community because they are able to make various kinds of processed foods from coffee. It's just that more intensive training is needed so that these processed products can support Bondowoso tourism.
The Development of Disaster Awareness in Primary Schools to Promote Awareness among Kelompok Kerja Guru (Teacher Working Group) 03 of Silo Sub-district in Jember
Authors: Finali Zetti , A'la Muh Zulfatul , Agustiningsih Agustiningsih
Published: November 2019

This project aims to raise awareness in the Kelompok Kerja Guru or KKG (Teacher Working Group) 03 Silo sub-district, Jember by developing Disaster Awareness in Primary Schools. Efforts to boost awareness are done through implementing training in the preparation of disaster-based learning tools and actions that must be taken by the teachers if a disaster occurs. The methods used in this service were (1) discussion on preparedness efforts and the level of teacher's awareness for disasters, (2) teachers training on the concept of disaster awareness in primary schools in the KKG 03 using modules, and (3) teachers assistance in transferring knowledge to students.