bluuebunny/crossref_metadata_2025 huggingface dataset:
Population pharmacokinetic analysis of transdermal granisetron in healthy Chinese and Caucasian volunteers
Authors: Li Jiayu , Hu Pei , Zhou Li , Nagahama Fumiko , Chen Rui
Published: June 2023

Granisetron patches are a prolonged delivery transdermal system that is used to prevent Chemotherapy-induced nausea and vomiting (CINV). To date, no pharmacokinetics comparison between Chinese and Caucasian populations has been conducted for granisetron patches. This study focused on the ethnic differences in pharmacokinetics (PK) of granisetron transdermal delivery system (GTDS) between Chinese and Caucasians and the influence of demographic covariates on pharmacokinetics (age, weight, height, body mass index, sex). To achieve this, blood concentration data were collected from 112 Caucasian healthy subjects participating in four clinical trials and 24 Chinese healthy subjects from one clinical trial, after a single application of the granisetron transdermal delivery system. A nonlinear mixed-effects model method of Phoenix NLME software was used to establish a population pharmacokinetic (Pop PK) model for Caucasian subjects. Bootstrap and visual predictive check (VPC) were used to validate the model. Based on the analysis a one-compartment model with first-order absorption and a first-order elimination well described the PK characteristics of GTDS. The apparent systemic clearance was determined to be 31316.3 mL/h and the central compartment volume of distribution was 6299.03 L. None of the five covariates (age, weight, height, body mass index, and sex) included in the Pop PK were significant covariates affecting PK. The final Pop PK model was used to simulate the Caucasian blood concentration by applying the dosing regimen used for the Chinese population. Comparison of the simulated Caucasian PK data with observed clinical PK data from Chinese healthy subjects revealed no significant differences in the main parameters, AUClast and Cavg, between the two groups. These findings suggested that no dose adjustment was required when applied to the Chinese population. In conclusion, this Pop PK study comparing the transdermal patch in Chinese and Caucasian healthy subjects provided valuable insights for optimizing dosage across ethnicities.
HECT, UBA and WWE domain containing 1 represses cholesterol efflux during CD4+ T cell activation in Sjögren’s syndrome
Authors: Yin Junhao , Xu Jiabao , Chen Changyu , Ma Xinyi , Zhu Hanyi , Xie Lisong , Wang Baoli , Shao Yanxiong , Zhao Yijie , Wei Yu , Hu Anni , Zheng Zhanglong , Yu Chuangqi , Fu Jiayao , Zheng Lingyan
Published: June 2023

Introduction: Sjögren’s syndrome (SS) is a chronic autoimmune disorder characterized by exocrine gland dysfunction, leading to loss of salivary function. Histological analysis of salivary glands from SS patients reveals a high infiltration of immune cells, particularly activated CD4+ T cells. Thus, interventions targeting abnormal activation of CD4+ T cells may provide promising therapeutic strategies for SS. Here, we demonstrate that Hect, uba, and wwe domain containing 1 (HUWE1), a member of the eukaryotic Hect E3 ubiquitin ligase family, plays a critical role in CD4+ T-cell activation and SS pathophysiology.

Methods: In the context of HUWE1 inhibition, we investigated the impact of the HUWE1 inhibitor BI8626 and sh-Huwe1 on CD4+ T cells in mice, focusing on the assessment of activation levels, proliferation capacity, and cholesterol abundance. Furthermore, we examined the therapeutic potential of BI8626 in NOD/ShiLtj mice and evaluated its efficacy as a treatment strategy.

Results: Inhibition of HUWE1 reduces ABCA1 ubiquitination and promotes cholesterol efflux, decreasing intracellular cholesterol and reducing the expression of phosphorylated ZAP-70, CD25, and other activation markers, culminating in the suppressed proliferation of CD4+ T cells. Moreover, pharmacological inhibition of HUWE1 significantly reduces CD4+ T-cell infiltration in the submandibular glands and improves salivary flow rate in NOD/ShiLtj mice.

Conclusion: These findings suggest that HUWE1 may regulate CD4+ T-cell activation and SS development by modulating ABCA1-mediated cholesterol efflux and presents a promising target for SS treatment.
No benefit of HIF prolyl hydroxylase inhibition for hypertensive renal damage in renovascular hypertensive rats
Authors: Hartner Andrea , Dambietz Thomas , Cordasic Nada , Willam Carsten , Burzlaff Nicolai , Brötsch Martin , Daniel Christoph , Schiffer Mario , Amann Kerstin , Veelken Roland , Schley Gunnar , Hilgers Karl F.
Published: June 2023

Introduction: We previously reported that malignant hypertension is associated with impaired capillary density of target organs. Here, we tested the hypothesis that stabilization of hypoxia-inducible factor (HIF) in a modified “preconditioning” approach prevents the development of malignant hypertension. To stabilize HIF, we employed pharmacological inhibition of HIF prolyl hydroxylases (PHD), that profoundly affect HIF metabolism.

Methods: Two-kidney, one-clip renovascular hypertension (2K1C) was induced in rats; controls were sham operated. 2K1C rats received either intermittent injections of the PHD inhibitor ICA (2-(1-chloro-4-hydroxyisoquinoline-3-carboxamido) acetate) or placebo. Thirty-five days after clipping, the frequency of malignant hypertension was assessed (based on weight loss and the occurrence of characteristic vascular lesions). In addition, kidney injury was compared between all ICA treated versus all placebo treated 2K1C, regardless of the occurrence of malignant hypertension. HIF stabilization was evaluated by immunohistochemistry, and HIF target gene expression by RT-PCR.

Results: Blood pressure was elevated to the same degree in ICA- and placebo-treated 2K1C compared to control rats. ICA treatment did not affect the frequency of malignant hypertension or the extent of kidney tissue fibrosis, inflammation, or capillary density. There was a trend towards higher mortality and worse kidney function in ICA-treated 2K1C rats. ICA increased the number of HIF-1α-positive renal tubular cell nuclei and induced several HIF-1 target genes. In contrast, expression of HIF-2α protein as well as HIF-2 target genes were markedly enhanced by 2K1C hypertension, irrespective of ICA treatment.

Discussion: We conclude that intermittent PHD inhibition did not ameliorate severe renovascular hypertension in rats. We speculate that the unexpected strong renal accumulation of HIF-2α in renovascular hypertension, which could not be further augmented by ICA, may contribute to the lack of a benefit from PHD inhibition.
The potential of SiK® fertilization in the resilience of chestnut plants to drought - a biochemical study
Authors: Carneiro-Carvalho Andreia , Pinto Teresa , Gomes-Laranjo José , Anjos Rosário
Published: June 2023

Silicon is an essential mineral nutrient, that plays a crucial role in the metabolic, biochemical, and functional mechanisms of many crops under environmental stress. In the current study, we evaluated the effect of SiK® fertilization on the biochemical defense response in plants exposed to water stress. Castanea sativa plants were fertilized with different concentrations of potassium silicate (0, 5, 7.5, and 10 mM of SiK®) and exposed to a non-irrigation phase and an irrigation phase. The results indicate that silicon promoted the synthesis of soluble proteins and decreased the proline content and the oxidative stress (reduced electrolyte leakage, lipid peroxidation, and hydrogen peroxide accumulation) in tissues, due to an increase in ascorbate peroxidase, catalase, and peroxidase activity, which was accompanied by the rise in total phenol compounds and the number of thiols under drought conditions. This study suggests that exogenous Si applications have a protective role in chestnut plants under water deficit by increasing their resilience to this abiotic stress
Boosting inhibition control process by knitting at school
Authors: Sonnier Frederic , Lussiana Emeline , Gueraud Sabine
Published: June 2023

Across two experiments, the presented research explored the impact of a knitting bout on elementary school pupils’ inhibition abilities. They proposed an accurate measure of the pupils’ inhibition abilities through the use of a stop-signal paradigm. In order to take into account, the differentiation between cool and hot inhibitions abilities, the emotional content of the stimuli was manipulated across experiments. Neutral materials were used in Experiment 1 when emotionally charged materials were in Experiment 2. The findings of both experiments highlighted a beneficial impact of the knitting bout on children’s inhibition abilities. While the results of Experiment 1 showed an optimization of inhibition abilities for the knitting session group in comparison to the control group, Experiment 2 revealed a disappearance of the effect of the emotional content on these abilities as well. Proposals as to why EF could be sensitive to knitting practice are discussed.
Caring for relations and organizational success—conceptualization of an Augustinian leadership scale
Authors: Slob Henri , van Geest Paul , Commandeur Harry
Published: June 2023

While making great strides in recent decades to connect leadership and human flourishing, the positive leadership literature has yet to focus on the aspect of the communal. Based on a close reading of Augustine’s works, this paper examines Augustinian leadership and emphasizes the importance of a view on leadership that aims at community building and contains an ethical framework characterized by veracity. This leadership style is founded on caritas (Gr.: agape, Eng.: love) as the main motive for leaders. Based on Augustine’s thinking, this kind of love is defined as a way to attain knowledge. We identify four subconstructs to constitute an Augustinian leadership scale: Centrality of the community, Veracity, Empathy and Success (through temperance). We provide theoretical grounds for the distinctiveness of this leadership construct as compared with neighboring constructs. Finally, we propose a testable framework of Augustinian leadership with a direct effect on affective commitment as well as a mediated effect, and with a sense of belonging as the mediating variable. We provide ideas for future research and present practical implications of the theoretical insights on Augustinian leadership.
Improving the therapist’s metacognition and capacity to intersubjectively attune with a patient with psychosis through the exploration of the therapist’s developmental history: a case report
Authors: Salvatore Gianpaolo , Di Somma Tania , Buonocore Luisa , Conza Maria , Di Sturco Nadia , Fimiani Gerardina , Manfredi Nicoletta , Marciano Raffaella , Pallotta Antonella , Proto Maria Grazia , Sateriale Anna
Published: June 2023

Clinical literature emphasizes how symptoms of psychosis can be efficiently targeted by psychological treatments. The most well-known approach to these symptoms is cognitive-behavioral therapy; but in the last few decades also other approaches are enriching the landscape, focusing on the dysfunctions in mentalization or metacognition, a spectrum of mental activities involving thinking about one’s own and others’ mental states. This huge amount of theoretical reflection and empirical research focused on the implementation of treatments does not seem to be associated with an attention to the inner world of the therapist who relates to the patient with psychosis; for example, to the impact of the therapist’s developmental history on the therapeutic relationship. In this paper the authors are inspired by an intersubjective perspective, according to which although the treatment is for the patient’s benefit, both the patient’s and the therapist’s developmental history and psychological organization are equally relevant for understanding the clinical exchange. On this basis, the authors make a “parallel” analysis of the clinical case of a young woman with symptoms of psychosis (i.e., persecutory delusions, auditory verbal hallucinations, social withdrawal) and its supervision process. They show how the therapeutic relationship can be significantly conditioned by the therapist’s developmental history; and how a process of supervision focused on the exploration of the traumatic elements of this history can effectively promote the therapist’s metacognitive capabilities, a functional patient-therapist intersubjective attunement, and a good clinical outcome.
Model of psychosocial determinants of health in processes of social exclusion
Authors: Cantos-Egea Jessica , Tous-Pallarés Jordi , Tierno-García Juana-María
Published: June 2023

The substantial increase in the number of families facing social exclusion in Europe and its direct relationship with health inequities is a challenge for studies approaching the social determinants of health and policies dealing with welfare and social inclusion. We start from the premise that reducing inequality (SDG10), has a value and contributes on other goals such as improving health and well-being (SDG3), ensuring quality education (SDG4), promoting gender equality (SDG5) and decent work (SDG8). In this study, we identify disruptive risk factors and psychological and social well-being factors that influence self-perceived health in trajectories of social exclusion. The research materials used a checklist of exclusion patterns, life cycles and disruptive risk factors, Goldberg’s General Health Questionnaire (GHQ-12), Ryff’s Psychological Well-being (PWB) Scale and Keyes’ Social Well-being Scale. The sample consists of 210 people (aged between 16 and 64  years): 107 people in a situation of social inclusion and 103 people in a situation of social exclusion. The data treatment involved statistical analysis, including correlation study and multiple regression analysis, aimed at developing a model of psychosocial factors that may act as health modulators, considering social factors as predictors in the regression model. The results showed that individuals in the sample, in a situation of social exclusion, have a greater accumulation of disruptive risk factors, and these are related to having fewer psychosocial and cognitive resources to cope with stressful situations: less self-acceptance, less mastery of the environment, less purpose in life, less level of social integration and social acceptance. Finally, analysis showed that in the absence of social integration and purpose in life, self-perceived health statuses decline. This work allows us to use the model obtained as a basis for confirming that there are dimensions of psychological and social well-being that should be considered stress-buffering factors in trajectories of social exclusion. These findings can help design psychoeducational programs for prevention and intervention with the aim of improving psychological adjustment and health states, as well as to promote proactive and reactive policies to reduce health inequalities.
Research on equity of medical resource allocation in Yangtze River Economic Belt under healthy China strategy
Authors: Ya-qing Liu , Hao-ran Niu , Xiang-yang Tian , Mei-cheng Zhang , Feng Jiang , Yu-tong Qian , Jian-bo Cao
Published: June 2023

Objective

This study aimed to assess the fairness of medical resource allocation in the Yangtze River Economic Belt, based on the Healthy China strategy. It aimed to identify the issues with resource allocation fairness and provide optimization suggestions.

Methods

To assess the allocation fairness from a geographical population perspective, the study used the Health Resource Concentration and Entropy Weight TOPSIS methods. Additionally, the study analyzed the allocation fairness from an economic level angle, using the Concentration Curve and Concentration Index.

Results

The study found that the downstream area had higher resource allocation fairness than the midstream and upstream areas. The middle reaches had more resources than the upper and lower reaches, based on population concentration. The Entropy Weight TOPSIS method found that Shanghai, Zhejiang, Chongqing, and Jiangsu had the highest comprehensive score index of agglomeration. Furthermore, from 2013 to 2019, the fairness of medical resource distribution gradually improved for different economic levels. Government health expenditure and medical beds were distributed more equitably, while general practitioners had the highest level of unfairness. However, except for medical and health institutions, traditional Chinese medicine institutions, and primary health institutions, other medical resources were mostly distributed to areas with better economic conditions.

Conclusion

The study found that the fairness of medical resource allocation in the Yangtze River Economic Belt varied greatly based on geographical population distribution, with inadequate spatial accessibility and service accessibility. Although the fairness of distribution based on economic levels improved over time, medical resources were still concentrated in better economic areas. The study recommends improving regional coordinated development to enhance the fairness of medical resource allocation in the Yangtze River Economic Belt.
A Conversation with ChatGPT about Digital Leadership and Technology Integration: Comparative Analysis Based on Human–AI Collaboration
Authors: Karakose Turgut , Demirkol Murat , Yirci Ramazan , Polat Hakan , Ozdemir Tuncay Yavuz , Tülübaş Tijen
Published: June 2023

Artificial intelligence (AI) is one of the ground-breaking innovations of the 21st century that has accelerated the digitalization of societies. ChatGPT is a newer form of AI-based large language model that can generate complex texts that are almost indistinguishable from human-generated text. It has already garnered substantial interest from people due to its potential utility in a variety of contexts. The current study was conducted to evaluate the utility of ChatGPT in generating accurate, clear, concise, and unbiased information that could support a scientific research process. To achieve this purpose, we initiated queries on both versions of ChatGPT regarding digital school leadership and teachers’ technology integration, two significant topics currently discussed in educational literature, under four categories: (1) the definition of digital leadership, (2) the digital leadership skills of school principals, (3) the factors affecting teachers’ technology integration, and (4) the impact of digital leadership on teachers’ technology integration. Next, we performed a comparative analysis of the responses generated by ChatGPT-3.5 and ChatGPT-4. The results showed that both versions were capable of providing satisfactory information compatible with the relevant literature. However, ChatGPT-4 provided more comprehensive and categorical information as compared to ChatGPT-3.5, which produced responses that were more superficial and short-cut. Although the results are promising in aiding the research process with AI-based technologies, we should also caution that, in their current form, these tools are still in their infancy, and there is a long way to go before they become fully capable of supporting scientific work. Meanwhile, it is significant that researchers continue to develop the relevant knowledge base to support the responsible, safe, and ethical integration of these technologies into the process of scientific knowledge creation, as Pandora’s box has already been opened, releasing newer opportunities and risks to be tackled.
Effects of Dairy Cows Management Systems on the Physicochemical and Nutritional Quality of Milk and Yogurt, in a North-Eastern Romanian Farm
Authors: Rațu Roxana Nicoleta , Cârlescu Petru Marian , Usturoi Marius Giorgi , Lipșa Florin Daniel , Veleșcu Ionuț Dumitru , Arsenoaia Vlad Nicolae , Florea Andreea Mihaela , Ciobanu Marius Mihai , Radu-Rusu Răzvan-Mihail , Postolache Alina Narcisa , Simeanu Daniel
Published: June 2023

The study’s objective was to investigate changes in the fatty acid composition of cow milk in general and in 80 Romanian Spotted cows’ husbandry and feeding systems in particular (grazing–GC group vs. stabulation–SC group). The ultimate objective was to determine if the changes that happened in the milk also transferred to the finished product. Also, the influence of the quality of raw milk produced by both systems was evaluated when yogurt was made from it. The milk was gathered in May, July and September and used for both the yogurt-making process and the study, which lasted from May to October. In comparison to milk from SC, milk from grazed caws had larger percentages of fat and dry matter throughout the summer (GC) season. Moreover, pasture-based rations (MGC) contained more PUFA than MCS did. Data research revealed that not only do factors such as milk origin and initial quality have a substantial impact on yogurt quality parameters, but also technologies such as milk fermentation have a considerable impact on the fatty acid profile of yogurt. In comparison to cows kept permanently in stables, grazed cows (MGC) had fat with a lower concentration of saturated fatty acids and a higher proportion of rumenic, vaccenic and oleic acids (MSC). When fresh milk is processed into yogurt and other dairy products, the fatty acid profiles alter, with saturated fatty acids predominating over unsaturated ones. The findings show that pasture-fed cows have a positive impact on milk quality, particularly in terms of fatty acid profile, as well as on yogurt’s ultimate nutritional and dietary quality.
From Large to Powerful: International Comparison, Challenges and Strategic Choices for China’s Livestock Industry
Authors: Shi Zizhong , Li Junru , Hu Xiangdong
Published: June 2023

Accelerating the construction of a strong livestock industry is of great significance to better guarantee the supply of livestock products and improve the quality, efficiency and competitiveness of the industry. This study constructed an evaluation index system including supply security, scientific and technological support, industrial resilience and international trade to evaluate the strength of China’s livestock industry, and then conducted an in-depth analysis of the issues and challenges of the construction of a livestock powerhouse. The research results showed that China’s livestock industry ranked 5th in the world, such that China was transitioning from being a large-producing livestock country to being a livestock powerhouse, although improvement was still needed to reach the goal. There were significant differences across species. China’s layer industry was a world leader; the pig, sheep and goat, and broiler industries were strong; and the beef cattle and dairy industries were weaker. There are still many challenges, such as the fact that the domestic supply security capacity needs to be strengthened, the level of scientific and technological support needs to be improved, the modern operation system needs to be sound, the industry and supply chain are not highly resilient, the international trade risks are increasing, and the policy support system needs to be improved. It is recommended to improve the institutional mechanism for the construction of the livestock industry, promote a high level of self-reliance and self-improvement in science and technology, build a modern livestock operation system, enhance the resilience and security level of the industry and supply chain, and consolidate and expand international trade and cooperation.
Has Rural Public Services Weakened Population Migration in the Sichuan–Chongqing Region? Spatiotemporal Association Patterns and Their Influencing Factors
Authors: Zhou Qianli , Zhang Shaoyao , Deng Wei , Wang Junfeng
Published: June 2023

The association between rural public services (RPSs) and population migration (PM) has become a key aspect of rapid urbanization in developing countries and an important breakthrough for improving rural–urban relations. An in-depth analysis of the heterogeneity of the weakening effect of RPSs on PM at different transformation phases and the internal mechanism of the evolution of association patterns driven by RPSs and PM helps to ensure better co-ordinated urban and rural development. This paper establishes an interactive analysis framework for measuring the spatiotemporal association and regional differences between RPSs and PM in the Sichuan–Chongqing region (SCR), and reveals the influence mechanism by employing multiscale geographically weighted regression (MGWR). The results indicate that the association rapidly increased with clear spatial heterogeneity across topographic units and the weakening effect of RPSs on PM begin to diverge during the urban–rural transition. The natural, economic, social, and urban–rural disparity factors in terms of the association exhibit significant spatial variability. In mountainous areas, where topography dominates, RPSs fail to effectively weaken rural migration. However, in the plain areas, urbanization is the main driver of urban–rural transition, and the adaptive upgrading and transformation of RPSs has made their weakening effect stronger, thus alleviating rural exodus and increasing population concentration. All these findings show that differentiated optimization strategies adhering to the association trends should be proposed for a deeper integration of rural revitalization and new urbanization in the SCR.
Do Pesticide Retailers’ Recommendations Aggravate Pesticide Overuse? Evidence from Rural China
Authors: Sun Shengyang , Zhang Chao , Hu Ruifa , Liu Jian
Published: June 2023

In rural China, pesticide retailers are often accused of recommending farmers apply more pesticides than the scientifically recommended rate, while playing an important role in providing technical information regarding pesticide use to farmers. However, there is little empirical evidence on the relationship between pesticide retailers’ recommendations and farmers’ pesticide overuse. Using survey data from 1084 rice farmers in four provinces, this study utilized an endogenous switching probit model to examine the impact of pesticide retailers’ recommendations on the overuse of pesticides at the level of pest-control observation, accounting for potential self-selectivity bias. Results show that the proportion of pesticide overuse at the level of pest-control observation for controlling major pests, secondary pests, and weeds is 58.5, 55, and 40.6%, respectively. Pesticide retailers’ recommendations are found to increase the probability of pesticide overuse at the level of pest-control observation for controlling major pests, secondary pests, and weeds by 62.1, 59.3, and 58.3%, respectively. The robustness check using a conditional mixed process model provided consistent findings. Accordingly, this study proposes that more efforts should be made to provide additional technology training activities for pesticide retailers, strengthen regulations on pesticide retailers’ information recommendations, and further improve socialized agricultural technology services.
Influence of Structure and Texture Feature on Retrieval of Ramie Leaf Area Index
Authors: Fu Hongyu , Lu Jianning , Chen Jianfu , Wang Wei , Cui Guoxian , She Wei
Published: June 2023

Leaf area index (LAI), as one of the important crop field phenotypes, plays a crucial role in evaluating crop growth and yield. The remote sensing method for crop parameters monitoring makes it possible to obtain crop LAI in a large area, efficiently and non-destructively. Fusion of spectral features, structure features, and texture features obtained by remote sensing images can effectively improve LAI estimation performance, but the impact of crop structure and texture feature differences on LAI remote sensing monitoring remains unclear. In this study, ramie was classified according to the differences of canopy structure, and then the LAI inversion performance of ramie was evaluated under different datasets to determine the contribution of structure features (canopy coverage, plant height) and texture features derived from remote sensing images to LAI estimation. Finally, multi-feature remote sensing indexes were integrated to construct the optimal ramie LAI estimation model. The results showed that structure features and texture features had an effect on ramie LAI inversion performance, and there were significant differences in LAI estimation accuracy among different data sets. A single structure feature or texture feature could not significantly optimize LAI inversion performance, while multi-feature fusion could effectively improve LAI estimation accuracy (R2 = 0.776, RMSE = 0.740). This study provides a robust, practical, and low-cost method for ramie LAI estimation, and provides a reference for crop LAI inversion research based on structural features and texture features.
Synthesis of Benzoxazinones Sulphur Analogs and Their Application as Bioherbicides: 1.4-Benzothiazinones and 1.4-Benzoxathianones for Weed Control
Authors: Mejías Francisco J. R. , Schwaiger Stefan , Varela Rosa M. , Molinillo José M. G. , Chinchilla Nuria , Macías Francisco A.
Published: June 2023

Eight different compounds inspired by benzoxazinones were synthesized in one simple step with easy purification. These compounds have a sulfur atom instead of the oxygen atom present in benzoxazinones. Furthermore, a new derivative obtained by a Rutkauskas–Beresnevicius reaction was synthesized. These compounds were evaluated in vitro to assess their phytotoxicity in plant cells by the elongation of wheat coleoptiles. The novel compounds showed higher inhibition than benzoxazinones and the positive control, especially at higher concentrations (1000 and 300 μM). Benzoxazinones have been described as histidine deacetylase inhibitors and we therefore evaluated the effect of 1.4-benzothiazinones and 1.4-benzoxathianones against HDA6, one of the most important enzymes of the family, in silico by molecular docking and molecular dynamics. In vitro studies against Echinochloa crus-galli, Lolium rigidum and Portulaca oleracea weeds gave interesting results against the growth of the roots for both monocots and dicots. Specifically, the inhibition was more pronounced against dicots, as in the case of common purslane, whose inhibition at a concentration of 1000 μM was similar to that of the classical herbicide employed as a positive control. Higher inhibition was obtained when an aliphatic group was present in the C2 position of 1.4-benzothiazinones.
Assessment of the Spatial Variability and Uncertainty of Shreddable Pruning Biomass in an Olive Grove Based on Canopy Volume and Tree Projected Area
Authors: Rodríguez-Lizana Antonio , Ramos Alzira , Pereira María João , Soares Amílcar , Ribeiro Manuel Castro
Published: June 2023

Olive pruning residues are a by-product that can be applied to soil or used for energy production in a circular economy model. Its benefits depend on the amount of pruning, which varies greatly within farms. This study aimed to investigate the spatial variability of shreddable olive pruning in a traditional olive grove in Córdoba (Spain) with an area of 15 ha and trees distanced 12.5 m from each other. To model the spatial variability of shreddable olive pruning, geostatistical methods of stochastic simulation were applied to three correlated variables measured on sampled trees: the crown projected area (n = 928 trees), the crown volume (n = 167) and the amount of shreddable pruning (n = 59). Pearson’s correlation between pairs of variables varied from 0.71 to 0.76. The amount of pruning showed great variability, ranging from 7.6 to 76 kg tree−1, with a mean value of 37 kg tree−1. Using exponential and spherical variogram models, the spatial continuity of the variables under study was established. Shreddable dry pruning weight values showed spatial autocorrelation up to 180 m. The spatial uncertainty of the estimation was obtained using sequential simulation algorithms. Stochastic simulation algorithms provided 150 possible images of the amount of shreddable pruning on the farm, using tree projected area and crown volume as secondary information. The interquartile range and 90% prediction interval were used as indicators of the uncertainty around the mean value. Uncertainty validation was performed using accuracy plots and the associated G-statistic. Results indicate with high confidence (i.e., low uncertainty) that shreddable dry pruning weight in the mid-western area of the farm will be much lower than the rest of the farm. In the same way, results show with high confidence that dry pruning weight will be much higher in a small area in the middle east of the farm. The values of the G-statistic ranged between 0.89 and 0.90 in the tests performed. The joint use of crown volume and projected areas is valuable in estimating the spatial variability of the amount of pruning. The study shows that the use of prediction intervals enables the evaluation of farm areas and informed management decisions with a low level of risk. The methodology proposed in this work can be extrapolated to other 3D crops without requiring modifications. On a larger scale, it can be useful for predicting optimal locations for biomass plants, areas with high potential as carbon sinks or areas requiring special soil protection measures.
Anatomical, Histological and Histochemical Observations of the Eyelids and Orbital Glands in the Lowland Tapir (Tapirus terrestris Linnaeus, 1785) (Perissodactyla: Ceratomorpha)
Authors: Klećkowska-Nawrot Joanna , Goździewska-Harłajczuk Karolina , Kupczyńska Marta , Kaleta-Kuratewicz Katarzyna , Kuropka Piotr , Barszcz Karolina
Published: June 2023

The lowland tapir is one of four species belonging to the Tapiridae family of the Ceratomorpha suborder, similar to Rhinocerotidae. This study describes anatomy with morphometry, histology (hematoxylin and eosin, Masson-Goldner trichrome, Movat pentachrome, mucicarmine, picro-Mallory trichrome) and histochemistry (PAS, AB pH 1.0, AB pH 2.5; AB pH2.5/PAS and HDI) of the upper and lower eyelids, and superficial gland of the third eyelid with the third eyelid, deep gland of the third eyelid, and lacrimal gland. The aim of the work is to show the features of the above-mentioned structures typical only for Tapiridae, as well as to show the presence of similarities and differences between the families forming the order Perissodactyla. The eyelashes on the upper eyelid were long, while those of the lower eyelid were short and much less prominent. In the upper and lower eyelid sebaceous glands, a characteristic simple alveolar gland producing a mucus-like secretion and poorly developed tarsal glands were observed. The marginal zone of the posterior surface of the eyelids was covered by stratified columnar epithelium with 18–21 layers of nucleated cells, while the bulbar zone of these surfaces was covered by cubic multilayer epithelium with 6–11 non-keratinized layers of cells and with sparse goblet cells. In only lower eyelids, numerous lymphoid nodules, diffuse lymphocytes and high endothelial venules were observed. The superficial gland was an acinar complex which secreted mucous and contained plasma cells within the interlobular and interlobular connective tissue. The upper and lower branches of the third eyelid were the shape of a bent “caudal fin” and were composed of hyaline cartilage, and they contained conjunctiva associated lymphoid tissue (CALT). The deep gland was also an acinar complex producing a serous character and having numerous diffuse lymphocytes. The lacrimal gland was an acinar complex producing seromucous secretions and had numerous plasma cells located in the glandular interstitium. The results of our research indicate that the features of the anatomy of the eyelids and orbital region in the lowland tapir are also typical of the family Tapiridae, but also have features common to the families Equidae and Rhinocerotidae. We confirm the presence of poorly developed tarsal glands in both eyelids as well as presence of a palpebral part of the lacrimal gland in the upper eyelid, which is typical only to Tapirus terrestris.
Protozoan Parasites of Sarcocystis spp. in Rodents from Commercial Orchards
Authors: Prakas Petras , Stirkė Vitalijus , Šneideris Donatas , Rakauskaitė Paulina , Butkauskas Dalius , Balčiauskas Linas
Published: June 2023

Small mammals are an important group of wildlife that can transmit pathogens to humans and animals. There is a lack of comprehensive studies on the protozoan parasites of the genus Sarcocystis in agricultural areas. The aim of the current research was to evaluate the prevalence of Sarcocystis spp., and to identify the parasite species found in the skeletal muscles of rodents and insectivores from commercial orchards. A total of 679 muscle samples from small mammals, mainly rodents (n = 674), belonging to eight species were examined. Muscle samples were pooled into groups, then digested, and the presence of the Sarcocystis species was confirmed by molecular methods. The examined parasites were determined in five rodent species, Apodemus agrarius, A. flavicollis, Clethrionomys glareolus, Microtus arvalis, and M. oeconomus. The prevalence of Sarcocystis spp. was low: 2.23% in voles and 0.79% in mice. Based on a sequence comparison of cox1 and 28S rDNA, four species were identified: S. myodes, Sarcocystis cf. strixi, Sarcocystis sp. Rod1, and Sarcocystis sp. Rod2. This is the first report of S. myodes in A. agrarius, A. flavicollis, and M. arvalis. The identified species were most closely related to Sarcocystis spp., and were transmitted by predatory mammals and birds. Future studies are needed to describe the species morphologically, as well as to define the host spectrum and to evaluate their possible pathogenicity.
Identification of SNPs Associated with Goose Meat Quality Traits Using a Genome-Wide Association Study Approach
Authors: Gao Guangliang , Zhang Keshan , Huang Ping , Zhao Xianzhi , Li Qin , Xie Youhui , Yin Chunhui , Li Jing , Wang Zhen , Zhong Hang , Xue Jiajia , Chen Zhuping , Wu Xianwen , Wang Qigui
Published: June 2023

(1) Background: Goose meat is highly valued for its economic significance and vast market potential due to its desirable qualities, including a rich nutritional profile, tender texture, relatively low-fat content, and high levels of beneficial unsaturated fatty acids. However, there is an urgent need to improve goose breeding by identifying molecular markers associated with meat quality. (2) Methods: We evaluated meat quality traits, such as meat color, shear force (SF), cooking loss rate (CLR), and crude fat content (CFC), in a population of 215 male Sichuan white geese at 70 days of age. A GWAS was performed to identify potential molecular markers associated with goose meat quality. Furthermore, the selected SNPs linked to meat quality traits were genotyped using the MALDI-TOP MS method. (3) Results: A dataset of 2601.19 Gb of WGS data was obtained from 215 individuals, with an average sequencing depth of 10.89×. The GWAS revealed the identification of 43 potentially significant SNP markers associated with meat quality traits in the Sichuan white goose population. Additionally, 28 genes were identified as important candidate genes for meat quality. The gene enrichment analysis indicated a substantial enrichment of genes within a 1Mb vicinity of SNPs in both the protein digestion and absorption pathway and the Glycerolipid metabolism pathway. (4) Conclusion: This study provides valuable insights into the genetic and molecular mechanisms underlying goose meat quality traits, offering crucial references for molecular breeding in this field.
Effects of Different Dietary Starch Sources and Digestible Lysine Levels on Carcass Traits, Serum Metabolites, Liver Lipid and Breast Muscle Protein Metabolism in Broiler Chickens
Authors: Luo Caiwei , Chen Yanhong , Yin Dafei , Yuan Jianmin
Published: June 2023

This study investigated the effects of digestible lysine (dLys) in different dietary starch sources on liver lipid metabolism and breast muscle protein metabolism in broiler chickens. The experimental design was a 3 × 3 two-factor completely randomized design. A total of 702 one-day-old male Arbor Acres Plus broilers were randomly divided into nine treatments of six replicate cages with thirteen birds each. The treatments consisted of three different starch sources (corn, cassava and waxy corn) with three different dLys levels (1.08%, 1.20% and 1.32%). The trial lasted from 1 to 21 days. Carcass traits, serum metabolites, breast muscle protein and liver lipid metabolism were evaluated. A significant interaction effect (p < 0.05) for dietary starch sources and dLys levels was noted in the percentage of abdominal fat and gene expression related to breast muscle protein metabolism throughout the experimental period. The waxy corn starch diet and a 1.08% dLys level in the diet increased both the percentage of abdominal fat (p < 0.01) and blood total cholesterol (p < 0.05) in the broilers. The waxy corn starch diet significantly upregulated the mRNA expressions of Eif4E, AMPK, FABP1, ACC and CPT1 (p < 0.05). The 1.32% dLys level significantly upregulated the mRNA expressions of mTOR, S6K1, Eif4E, AMPK and PPARα (p < 0.05) and significantly downregulated the mRNA expressions of MuRF and Atrogin-1 (p < 0.05). In summary, the waxy corn starch diet resulted in significantly higher expression levels of fat-synthesis-related genes than lipolysis-related genes, leading to abdominal fat deposition in broilers. Increasing the level of dLys in the diet increased the protein content in muscle by promoting protein synthesis and inhibiting protein degradation and also promoted the expression of lipolysis-related genes, thereby degrading the generation of abdominal fat in broilers. Our findings signify that increasing the dLys level to 1.32% when using the waxy corn starch diet could improve carcass traits.
Composition, Diversity and Sex-Related Differences in Intestinal Microbiota in Captive African Penguins (Spheniscus demersus)
Authors: Jiang Jingle
Published: June 2023

An understanding of the microbial communities in African penguins (Spheniscus demersus) could provide valuable information for saving this endangered species. The objective of this study was to investigate the composition, diversity and sex-related differences in the intestinal microbiota of captive African penguins. Fecal samples were collected from 21 captive adult African penguins reared in the same conditions at Shanghai Zoo. The results show that Proteobacteria, Actinobacteria and Firmicutes were the predominant bacteria in the intestinal microbiota of the captive African penguins. No difference was found in microbial diversity between female and male African penguins, as shown by their similar alpha and beta diversities. However, a notable sex-related difference was found between their microbial compositions. Female African penguins have a higher abundance of Pseudomonas and a lower abundance of Kocuria than males. A functional prediction indicates that the “mRNA surveillance pathway”, “Polyketide sugar unit biosynthesis”, “Wnt signaling pathway”, “Lysosome” and “Cell cycle” pathways were significantly enriched in the microbiota of female African penguins. In conclusion, the present study indicates that the compositions and predicted functions of the intestinal microbiota are significantly different between the sexes. Our data suggest that the intestinal microbiota of female African penguins are more unstable than the intestinal microbiota of males in captivity.
Effects of Dietary Chenodeoxycholic Acid Supplementation in a Low Fishmeal Diet Containing Clostridium autoethanogenum Protein on Growth, Lipid and Cholesterol Metabolism, and Hepatopancreas Health of Litopenaeus vannamei
Authors: Shi Menglin , Zheng Chaozhong , Sun Yidan , Li Xiaoyue , He Guilun , Cao Junming , Tan Beiping , Xie Shiwei
Published: June 2023

The study aimed to assess the impact of adding chenodeoxycholic acid (CDCA) to the diet of Litopenaeus vannamei on their growth performance, lipid and cholesterol metabolism, and hepatopancreas health while being fed a low fishmeal diet. Five diets were formulated, one of which contained 25% fishmeal (PC); fishmeal was partially replaced with Clostridium autoethanogenum protein in the remaining four diets and supplemented with 0, 0.03, 0.06, and 0.09% CDCA (NC, BA1, BA2, and BA3, respectively). In this study, four replicates of each diet were assigned and each replicate consisted of 30 shrimp with an average weight of (0.25 ± 0.03 g). The shrimp were fed four times a day for a period of 56 days. The results of this study indicate that the inclusion of CDCA in the diet had a positive impact on the growth performance of the shrimp. The final body weight (FBW), weight gain (WG), and specific growth rate (SGR) of the shrimp in the PC group were similar to those in the BA2 group, and significantly higher than those in the other three groups. The survival rate (SR) was similar among all groups. In comparison to the PC group, the low fishmeal groups exhibited a significant decrease in the crude lipid content of the whole shrimp, as well as the Total cholesterol (T-CHOL), Low-density lipoprotein (LDL-C), and High-density lipoprotein (HDL-C) levels in the hemolymph. Regarding the sterol metabolism, the dietary supplementation of CDCA up-regulated the mRNA expression of intracellular cholesterol transporter 1-like (npc1), 7-dehydrocholesterol reductase (7dhcr), Delta (24) sterol reductase (Δ24), HMG-CoA reductase membrane form (hmgcr), and sterol carrier protein 2 (scp). In the lipid metabolism, the mRNA expression of sterol-regulatory element binding protein (srebp) was significantly down-regulated in the shrimp fed the BA1 diet and the expression of AMP-activated protein kinase (ampk) was significantly up-regulated in the shrimp fed the BA1 and BA3 diets compared to the PC group. The mRNA expression of triacylglycerol lipase (tgl) was significantly up-regulated in the shrimp fed the BA2 diet compared to the NC group. Compared with the shrimp fed the PC diets, the dietary supplementation of CDCA significantly down-regulated the protein expression of SREBP1. The lumen damage in the BA1 group was significantly less severe than those in the NC group. The addition of 0.06% CDCA to low fishmeal diets can improve the growth performance, lipid and cholesterol metabolism, and hepatopancreas health of L. vannamei.
Antibacterial Activity of Dissolved Silver Fractions Released from Silver-Coated Titanium Dental Implant Abutments: A Study on Streptococcus mutans Biofilm Formation
Authors: Salaie Ranj Nadhim , Hassan Pakhshan A. , Meran Zhala Dara , Hamad Shehab Ahmed
Published: June 2023

(1) Background: The aim of this research was to investigate the antibacterial activity of dissolved silver from silver-coated titanium implants against Streptococcus mutans. (2) Methodology: Silver-coated titanium implant discs were immersed in 1.8 mL of brain heart infusion broth (BHIB) and incubated for 24 h in order to release the silver ions into the broth. The coating quality was confirmed via EDS, and the dissolved silver was measured via inductively coupled plasma mass spectrometry (ICP-MS). The experimental design used unconditioned broth (control) and broth conditioned with silver released from silver-coated titanium implants (n = 6). Regarding the antibacterial activity, isolated Streptococcus mutans was used. A turbidity test and lactate production test were performed to determine the effect of dissolved silver on bacterial growth in a suspension and biofilm formation. (3) Result: The results showed that the coating was successfully applied on the substrate. There was around 0.3 mg/L of silver released into the BHIB, and the turbidity of the control group was significantly higher than the treatment, with measured absorbance values of 1.4 and 0.8, respectively, indicating that the dissolved silver ions from the silver-coated titanium discs exhibited some degree of antibacterial activity by preventing the growth of Streptococcus mutans. However, the results of the antibiofilm activity test did not show any significant difference between the groups. (4) Conclusion: The dissolved silver from silver-coated titanium implants has an antibacterial activity but not a significant antimicrobial activity, indicating that the dissolved silver from silver-coated titanium abutments can significantly reduce the incidence of peri-implant mucositis.