bluuebunny/crossref_metadata_2025 huggingface dataset:
Peran Kepercayaan Diri dalam Memoderasi Pengaruh Pengetahuan Dasar Akuntansi, Prestasi Matematika, dan Sikap Belajar terhadap Pemahaman Akuntansi
Authors: Fadhilah Nurul
Published: November 2024

Penelitian ini bertujuan untuk menganalisis pengaruh kemampuan akuntansi, hasil belajar matematika, dan perilaku belajar terhadap tingkat pemahaman akuntansi secara parsial, serta peran kepercayaan diri dalam memoderasi hubungan antara kemampuan dasar akuntansi, hasil belajar matematika, dan perilaku belajar terhadap pemahaman akuntansi. Responden penelitian terdiri dari 105 siswa akuntansi kelas X. Teknik analisis data menggunakan software WarpPLS 6.0, dengan hasil penelitian sebagai berikut: (1) kemampuan dasar akuntansi dan perilaku belajar tidak berhubungan secara parsial dengan tingkat pemahaman akuntansi, (2) terdapat pengaruh signifikan antara hasil belajar matematika dan pemahaman akuntansi, (3) kepercayaan diri tidak memoderasi hubungan antara kemampuan dasar akuntansi, hasil belajar matematika, dan perilaku belajar terhadap tingkat pemahaman akuntansi. Penelitian selanjutnya disarankan untuk menggunakan aspek-aspek lain yang berkaitan dengan pemahaman akuntansi dan mengembangkan variabel penelitian lebih lanjut.
Entrepreneurship-Based Education: Teaching Students to Think Creatively and Innovatively
Authors: Wakhudin Wakhudin , Basri Hasan , Wahyudi Andri , Ginanjar Martin Anjar
Published: November 2024

Entrepreneurship-based education in the era of globalization and fast-developing technology is becoming increasingly important for the younger generation to face the challenges of a dynamic world of work. The purpose of this study is to explore the effectiveness of entrepreneurship education in shaping an innovative mindset among students. The methods used in this study include qualitative analysis through a literature review approach to analyze and summarize various relevant sources on entrepreneurship education and the development of creative and innovative thinking skills in students. The results showed that entrepreneurship-based education successfully improved students' skills in identifying opportunities, solving problems creatively, and adapting to change. The implication of this study confirms that the integration of entrepreneurship education in the curriculum can significantly contribute to the development of relevant skills for students, as well as encourage innovation and economic growth in society. Further research is recommended to explore different approaches in teaching entrepreneurship and their impact on students' long-term development.
Exploring the Relationship between Teacher Professional Development and Student Learning Outcomes
Authors: Dewi A. Erni Ratna , Kalil Nur Cahyani , Hidayat Hidayat , Juniati Sri Rahayu
Published: November 2024

Teacher professional development is a key factor in improving education quality and student learning outcomes. This study aims to explore the relationship between teacher professional development and student learning outcomes and identify factors that influence the effectiveness of professional development programs. The method used in this research is a literature review that includes analyzing various studies related to teacher professional development and its impact on student learning outcomes. This study collected data from relevant articles, journals and research reports. The results of the review show that teacher professional development contributes significantly to improving student learning outcomes through improving pedagogical competence, technology utilization and classroom management. However, there are obstacles such as a lack of teacher motivation that affects the effectiveness of the program. This study provides important implications for stakeholders, showing the need for collaboration between the government, educational institutions and teachers in creating a sustainable professional development system. With adequate support, it is expected that professional development can effectively improve the quality of education and student learning outcomes, as well as create a learning environment that is more inclusive and responsive to students' needs.
The Role of Social Media in Increasing Brand Awareness: A Literature Review
Authors: Suparto Yuyun Hadi
Published: November 2024

Social media has become an important platform in modern marketing strategies, especially in the context of increasing brand awareness. This research aims to investigate how social media can effectively increase brand awareness through visual content strategies, the use of influencers, and employee engagement. This research uses a literature review approach. This method aims to analyze and summarize existing ideas and knowledge related to the research topic and look for gaps for further research. This literature review is based on relevant academic sources, which can explain the latest thinking and identify theories related to the research. The results of the literature review show that social media is a very effective tool in building brand awareness in the current digital era. By using the right strategies, such as leveraging engaging visual content, collaborating with relevant influencers, and encouraging employee engagement in sharing company content, brands can reach a wider audience and increase brand recognition. The implications of this research emphasize the importance of companies adapting social media strategies that are responsive to changing trends and consumer preferences, so that they can maximize the influence of social media in strengthening brand image and achieving broader marketing goals.
Percaya Diri Memoderasi Pengaruh Dasar Akuntansi, Hasil Belajar Matematika, dan Perilaku Belajar Terhadap Pemahaman Akuntansi
Authors: Anggraini Tri Chandra
Published: November 2024

Penelitian ini bertujuan untuk menganalisis pengaruh kemampuan akuntasi hasil belajar matematika dan perilaku belajar terhadap Tingkat pemahaman akuntansi secara parsial dan kepercayaan diri dapat memoderasi hubungan antara kemampuan dasar akuntansi, hasil belajar matematika dan perilaku belajar terhadap pemahaman akuntansi. Responden penelitian ini berjumlah 105 siswa akuntansi kelas X dengan Teknik analisis data menggunakan software warpPLS 6.0 dengan hasil Penelitian menunjukkan bahwa: (1) tidak ada hubungan kemampuan dasar akuntansi dan perilaku belajar dengan tingkat pemahaman akuntansi secara parsial (2) terdapat pengaruh antara hasil belajar siswa matematika dengan pemahaman tentang akuntansi (3) kepercayaan diri bukan merupakan variabel moderasi antara kemampuan dasar akuntansi, hasil belajar dari matematika, dan perilaku belajar terhadap tingkat pemahaman akuntansi secara parsial. Saran untuk penelitian selanjutnya dapat menggunakan aspek lain yang berkaitan dengan pemahaman akuntansi selain yang digunakan dalam penelitian ini dan dapat mengembangkan
Is Personal Branding The Key To Success In Simultaneous Elections In 2024?
Authors: Kusnindar Arum Arupi , Sari Ayunita , Fitria Bilqis Dwi , Pratama Krisna Fino
Published: November 2024

In the context of representative democracy, elections play a central role in determining the direction of a country's policies and leadership. In Indonesia, the moment of general elections, especially the presidential and vice presidential elections, is very important to determine the political future of the nation. With the development of democracy, the phenomenon of first-time voters has emerged-a new generation that is actively involved in the political process. This study aims to examine the impact of personal branding of presidential and vice presidential candidates on novice voters in the 2023 general election. The method used is quantitative with data collection through questionnaires and data analysis using Smart PLS. The results showed that personal attributes such as honesty, professional background, populism, and wealth have a significant positive influence on the perception of personal branding of presidential and vice presidential candidates among novice voters. These findings make a significant contribution to the understanding of how personal branding elements can influence the decisions of novice voters in general elections.
Data Security Analysis of Chatting Applications Using End-to-End Encryption
Authors: Intan Juniarmi
Published: November 2024

This research aims to analyze data security in chat applications by applying end-to-end encryption techniques. The research method used is a literature study and qualitative analysis of chat applications that use end-to-end encryption. The results show that the implementation of end-to-end encryption in chat applications is effective in maintaining the confidentiality of user data, securing messages from hacking and data interception attacks, and increasing user security awareness. However, this study also identified some challenges related to application performance and encryption key management. Therefore, this study recommends the development of a more efficient and comprehensive end-to-end encryption technology to maximize user data security in chat applications.
The Impact of Error in Persona : Lilability of Police Investigators in Wrongful Arrest Cases
Authors: Gunawan Adi , Malau Parningotan , Khotami Wildani , Salmon Harly Clifford Jonas
Published: November 2024

Wrongful arrest in Indonesia is a serious problem in law enforcement that often results in physical suffering, psychological, and social losses for victims. One form of error that often occurs is “error in persona,” which is a misidentification that results in the arrest of an innocent person. This research uses normative juridical method with literature study and qualitative analysis to analyze the responsibility of investigators in cases of wrongful arrest. The purpose of this study is to understand the factors that cause errors in persona and their impact on victims and the legal system. The results show that misidentification often occurs due to inaccuracies in evidence collection, pressure to quickly resolve cases, and the use of inadequate investigative methods. In addition, the importance of fulfilling the legal procedures stipulated in the Criminal Procedure Code (KUHAP) is crucial to prevent these errors. Reforms in the investigation process, increased capacity of investigators, and the use of modern technology are necessary to minimize the risk of errors and ensure justice and the protection of human rights.
Exploring the Role of Notaries in Common Law and Civil Law Legal Systems: A Comparative Analysis of Authentic Deed Making
Authors: Nuroini Indi , Jamilah Akfah Zakiah , Azizi Agung Wildan , Sudrajat Ajeung Syilva Syara Noor Silmi
Published: November 2024

The role of notaries in Civil Law and Common Law legal systems, with a focus on Indonesia and Malaysia as examples of each. The Civil Law legal system in Indonesia regulates notaries as public officials responsible for making authentic deeds with strong evidentiary power in the eyes of the law. This study uses a normative research approach to explore the legal regulations governing notaries in both legal systems, by analyzing the UUJN in Indonesia and the Notaries Public Act 1959 in Malaysia. The main findings show that notaries in Civil Law have broader authority in making authentic deeds compared to Notary Public in Common Law, which focuses more on document verification and attestation. This difference reflects the characteristics of each legal system in regulating the role of notaries in maintaining legal certainty in society. Further research on these differences and similarities can provide valuable insights for legal development in both systems, as well as help formulate more effective strategies in meeting the increasingly complex and connected global legal needs.
Information System Security Analysis in the Era of Internet of Things (IoT)
Authors: Desi Ratna Sari
Published: November 2024

The analysis of information system security in the Internet of Things (IoT) era reveals the complexities and challenges associated with ensuring the integrity and security of an increasingly connected infrastructure. The research method used in this study is qualitative research, Data related to information system security in the context of IoT will be collected from various sources, including scientific literature, research reports, and technical documentation and analyzed in-depth literature. Serious threats such as malware, ransomware, and denial of service (DoS) attacks require a proactive and holistic approach in dealing with them. Lack of consistent security standards, device interoperability vulnerabilities, and lack of end-user security awareness are the main focus of efforts to improve security in IoT environments. Cooperation between industry, government, and other stakeholders is needed to develop robust security standards, increase user security awareness, and address interoperability challenges. Through these measures, it is hoped to create a safer and more secure IoT environment, maintaining data integrity and infrastructure security in an increasingly connected and complex era.
The Influence of Problem-Solving Skills and Self-Efficacy on Entrepreneurial Readiness in Culinary Skills Program
Authors: Astari Nesya Eka , Muslim Suyitno , Hasana Uswatun
Published: November 2024

This study examines the influence of problem-solving skills and self-efficacy on entrepreneurial readiness among participants in a culinary skills program. Given the increasing importance of entrepreneurship in the culinary industry, understanding these factors can enhance educational strategies and support aspiring culinary entrepreneurs. A quantitative research design was employed, utilizing a survey methodology to collect data from 125 culinary skill program participants. The survey measured participants' problem-solving skills, self-efficacy, and entrepreneurial readiness. Data were analyzed using multiple regression analysis to determine the relationships between these variables. The findings reveal that both problem-solving skills and self-efficacy significantly influence entrepreneurial readiness. Specifically, problem-solving skills were found to have a stronger impact, suggesting that participants who are adept at resolving challenges are more prepared for entrepreneurial ventures. Additionally, self-efficacy contributes positively to entrepreneurial readiness, indicating that confidence in one's abilities is crucial in entrepreneurial preparedness. The study concludes that enhancing problem-solving skills and self-efficacy in culinary education programs can significantly boost entrepreneurial readiness.
Implementation of TQM in Islamic-Based Schools: A Literature Review
Authors: Kusriyanto Kusriyanto , Wening Nur
Published: November 2024

The quality of Islamic education in Indonesia plays an important role in supporting the improvement of education quality nationally in order to produce quality human resources (HR). This qualitative research aims to determine the implementation of Total Quality Management (TQM) in Islamic-based schools. The research was conducted using qualitative research methods, especially Systemic Literature Review (SLR) using the results of previous research. Based on a literature review study, it was found that TQM can actually be implemented in the management of the Islamic education system. By: 1) The principle of customer focus and total involvement, namely: leadership and employee empowerment. 2) Leadership roles, education and training, support structures, communication, rewards and recognition, and measurement 3) Implementation of continuous improvement, meaning that quality is always improved and adapted to changing customer needs and desires.
The Effect of Service Quality and Patient Satisfaction on Patient Loyalty Mediated by Patient Trust at Byor Skin Clinic
Authors: Lepianda Jessica , Gunardi Wani Devita , Fushen Fushen
Published: November 2024

Nowadays, women in Indonesia are very fond of the beauty and smoothness of the skin of Korean artists, along with the increasing trend of drama and KPOP in Indonesia, many are competing to do beauty treatments to be able to maintain their appearance and have white, clean, bright, glowing skin or what is currently often called clear skin. The increasing awareness of self-beauty treatments are in line with the beauty industry in Indonesia which also continues to grow, it can be seen from the increasing number of people who visit and perform treatments at beauty clinics and the increasing number of beauty clinics that we can find. Data were obtained through questionnaires distributed randomly. The analysis technique was SEM and using AMOS software version 23. Based on the analysis results, it was found that out of 7 hypotheses, there was 1 hypothesis that rejected, and other 6 hypotheses were accepted. The service quality has no positive effect to the patient loyalty, the patient satisfaction has positive effect to patient loyalty, patient trust has positive effect to patient loyalty, service quality has positive effect to patient trust, patient satisfaction has a positive effect to patient trust, patient trust has positive effect mediate service quality with patient loyalty, and the patient trust also has positive effect mediate patient satisfaction with patient loyalty.
The Impact of Financial, Psychological, and Social Motivation on Employee Performance Accountability in the Cooperative and SME Office of Klaten Regency
Authors: Estiningtyastuti Estiningtyastuti , Putra Zai Simon Nisja , Prijanto Tulus , Hastuti Rining Dyah Tri
Published: November 2024

The objective of this research is to examine the influence of financial, psychological, and social motivation on employee performance accountability within the Cooperative and SME Agency of Klaten Regency. The study encompasses a total employee population of 230 individuals, among whom 64 have less than 5 years of work experience. The research employs Multiple Linear Regression Analysis as its methodology. The results indicate a positive effect of financial motivation on performance accountability, with psychological factors also demonstrating a significant positive impact. Conversely, social factors do not show a significant influence on performance accountability. Therefore, further research is necessary in the areas of psychology and finance, focusing on aspects such as work culture, organisational culture, and the implementation of internal control policies, to enhance employee performance accountability. This study aims to provide deeper insights into the factors affecting performance accountability and to offer valuable recommendations for improving employee performance based on the research findings.
Forming an Ethical Mindset: Applying Constructivist Learning to Teach Anti-Corruption Education in Junior High Schools
Authors: Yasmiatti Luh Wayan
Published: November 2024

Corruption is a widespread problem that disrupts the stability and progress of a country, including Indonesia, and undermines the economy and public trust. Education is essential to tackle corruption from an early age to foster a culture of integrity and ethical behavior. In this article, we talk about how constructivist learning theory can be used to teach anti-corruption education in junior high schools. The purpose of this study is to show how important constructivist methods such as experiential learning, value-based education, role play, and discussion are in encouraging moral reasoning and civic responsibility among young students. Recent studies on constructivist pedagogy and anti-corruption education were collected through literature review methodologies. The findings suggest that constructivism promotes deeper moral engagement and better critical thinking, which is essential for understanding and combating corrupt behavior. In addition, students internalize anti-corruption principles through real-world scenarios, discussions, and community projects. As this study shows, the constructivist approach offers a promising platform for teaching anti-corruption education because it allows students to participate in the learning process interactively and think critically. This study shows that schools should prioritize a constructivist approach in equipping students with the moral competencies necessary to build a non-corrupt society
System Development Methodology For Accounting Infoemation System
Authors: Putri Devi Dwi , Hazmi* Yusri , Yana Fitri , Nabella Reka , Saputri Yuni
Published: November 2024

This study discusses the importance of selecting an appropriate information system development method before starting the development process to ensure the creation of a system that meets expectations. Although there are many methodologies or models for software development, confusion often arises when choosing the most suitable one based on specific situations or environments. The article also outlines the basic concepts of systems, accounting information systems, and the stages of system analysis, including proposal preparation, system analysis implementation, and the preparation of system analysis reports. The research methodology used is library research, which involves studying literature and analyzing various written information sources. The findings highlight the importance of written documentation at each stage of system development to effectively communicate plans and findings to information users, as well as the significance of employing effective information-gathering techniques during system analysis. Therefore, selecting the right method and maintaining thorough documentation are crucial to successfully developing an information system that meets user needs and supports organizational goals.
Effect of Vitamin D3 Levels on Varicella-Zoster Virus Infection and IFN-Gamma Expression in Children: A Cross-Sectional Study in Iraq
Authors: Yousif Maitham G. , Al-Amran Fadhil G. , Rawaf Salman , Al-Jumeily Dhiya
Published: November 2024

Background: This study aimed to explore the correlation between Vitamin D3 levels and IFN-Gamma expression in children residing in the southern and central provinces of Iraq. Vitamin D3 plays a pivotal role in immune function, and IFN-Gamma is a crucial cytokine involved in antiviral defense. Investigating the connection between Vitamin D3 and IFN-Gamma offers valuable insights into immune responses and potential implications for infectious diseases.Methods: A case-control study was conducted, involving children from various schools and kindergartens in the southern and central provinces of Iraq. The study assessed Vitamin D3 levels and measured IFN-Gamma expression. Statistical analyses were performed to determine the relationship between these variables.Results: The outcomes revealed a significant positive correlation between Vitamin D3 levels and IFN-Gamma expression within the study population (p < 0.05). Children with higher Vitamin D3 levels exhibited elevated IFN-Gamma expression, suggesting a possible immunomodulatory impact of Vitamin D3 on IFN-Gamma production.Conclusion:These findings underscore the importance of maintaining adequate Vitamin D3 levels to support immune function, particularly in relation to IFN-Gamma expression. Improving Vitamin D3 status could potentially bolster antiviral defense mechanisms and reduce susceptibility to viral infections among children in the southern and central provinces of Iraq. Further investigation is warranted to delve into the underlying mechanisms and potential clinical ramifications of this significant association. Additionally, exploring the long-term effects of maintaining optimal Vitamin D3 levels on immune function and the outcomes of infectious diseases in this population would offer valuable insights for preventive and therapeutic strategies.
Risk factors for Beta-lactam and carbapenem-resistant bacteria causing post-burn infections
Authors: Haider Muhammad Hayat , Akram Amina , Nasir Maira , Sherwani Rehan Ahmad Khan , Shareef Muhammad Umair , Ali Muhammad Nauman , Riaz Saba
Published: November 2024

Background: Burn patients are predisposed to infection because of the loss of skin barrier protection and low immunity. This study aims to evaluate the  risk factors of post-burn infections due to beta-lactam and carbapenem-resistant bacteria (CR) isolated from a major burn unit.Methods: The descriptive study was conducted at Jinnah Hospital Lahore’s Burn and Reconstructive Surgery Centre (JB & RSC) and the Institute of Microbiology and Molecular Genetics (MMG), University of the Punjab, Lahore. The clinical specimens of wound swabs, including the age, gender, socio-economic status, residence, occupation, hospital stay, wards, burn degree, total burnt surface area (TBSA%), etiology of burn, and other factors were recorded. Antimicrobial susceptibility testing (AST), ESBL and CR screening (Beta- lactam and carbapenem resistant bacteria) were performed using CLSI guidelines 2017.Results: The most dominant strain was Pseudomonas spp. 26.5% (n=106), followed by 15% (n=60) Klebsiella spp., and 7.5% (n=30) Acinetobacter spp. with ESBL-producing strains. Compared to nosocomial infections, community-acquired infections were observed in 314 (78.5%) patients with more ESBL production. There was a significant relation between burnt people having an age of ≤ 29 years with ESBL (p=0.985) and carbapenemases (p=0.694) infections. None of the other factors, like low socioeconomic status and more hospital stays had a significant correlation (p > 0.05).Concluded: It has been concluded that MDR gram-negative strains play a serious role in burn infections and demand suitable methods to decrease their number. Pseudomonas spp. was the most common post-burn infection causing bacteria. Unfortunately, a high frequency of burn infections was observed in the patients at a young age.Keywords: Post burn infections; Gram negative; MDR; Beta- lactam; Carbapenem resistant
Bioremediation of hydrocarbon pollutants by Pseudomonas putida under optimal conditions
Authors: Shandookh Fatima Kareem , Mohammed Melad Khalaf , Jabbar Ahmed Darweesh
Published: November 2024

Background: The extreme toxicity of petroleum products to human and environmental health, particularly when linked to large-scale unintentional spills, has made them one of the most serious and current environmental pollution issue. Petroleum products are a complex mixture of hydrocarbons.Methods: Oil-contaminated soil samples were gathered and utilized to isolate hydrocarbon-degrading bacterial isolates. The bacterial isolates possessing bioremediation capabilities were identified using phenotypic determination and biochemical properties. The Optimal conditions including incubation period, temperature, different pH values and different carbon sources were studied for bioremediation of crude oil by bacterial isolates.Result: In Wasit province of Iraq, Pseudomonas putida, P. fluorescens, and P. aeruginosa isolated from soil samples tainted with petroleum hydrocarbons. Pseudomonas putida have more ability for bioremediation of crude oil (68%) compared to P. aeruginosa and P. fluorescens (47%, 58%) respectively. The results of optical density (600nm), biomass and (E24%) index for Pseudomonas putida were (1.080, 1.98 and 60%) respectively. The Optimal conditions (incubation period, temperature, different pH values and different carbon sources) were studied for bioremediation of crude oil by Pseudomonas putida by using liquid BHM supplemented with 1% crude oil. The result of this study showed that the incubation period of 9 days was the optimum for  bioremediation of hydrocarbons which was 88.33%. The optimum temperature and pH were 35 °C and 7 respectively. The carbon source (glucose and fructose) was optimal for hydrocarbon bioremediation.Conclusion: This bacterial isolate Pseudomonas putida can be use in petroleum hydrocarbon bioremediation process.Keywords: Pseudomonas putida; Bioremediation; Optical density
Characterization of garlic virus A, garlic virus D, and onion yellow dwarf virus infecting onion
Authors: Amer Mahmoud A. , Amir Muhammad , Hussain Khadim , Al-Shahwan Ibrahim M , Al-Saleh Mohammed A.
Published: November 2024

Background: Allium cepa is one of the major vegetable crops cultivated in Saudi Arabia. This economically important crop faces several biotic stresses which adversely affect the quality and quantity of its production. Several virus groups (potyviruses, carlaviruses, tospoviruses, and allexiviruses) have been reported infecting Allium crops.Methods: During the growing season of 2021-2022, a total of 81 onion samples exhibiting virus-like symptoms were collected from two different geographical regions in Saudi Arabia. The serological technique (ELISA) was used to detect the important allexiviruses and potyviruses. RT-PCR amplification of partial genome sequence was done using degenerate primers for allexiviruses and potyviruses and the phylogenetic trees were constructed using different bioinformatic tools.Results: The results obtained from ELISA tests showed that 26% and 32% of onion samples were positive with both Garlic Virus A (GarV-A) and Onion yellow dwarf virus (OYDV) respectively. RT-PCR amplification and sequencing results showed that two allexiviruses, GarV-A, garlic virus D (GarV-D), and one Potyvirus (OYDV) were detected in both regions. Sequence data were deposited in the GenBank database with accession numbers, OQ397545, OQ397546 for GarV-A, OQ397547 for GarV-D, and OQ397548, OQ397549 for OYDV, sequentially. Phylogenetic tree analysis of these virus isolates showed making clades with closely related isolates of their respective viruses. Pairwise nucleotide sequence identity showed their similarity with GarV-A, GarV-D, and OYDV isolates reported earlier in the GenBank.Conclusion: To the best of our knowledge, these two distinct allexiviruses (GarV-A, GarV-D) and one Potyvirus (OYDV) were isolated for the first time from an onion crop in Saudi Arabia.Keywords: Allexiviruses; Potyviruses; Serological Detection; Allium cepa; RT-PCR; Sequence
Evaluation of the effectiveness of Azolla fern in removing the toxicity of lead and cadmium in water
Authors: Muttaleb Wurood Hamzah , Altameme Huda Jasim
Published: November 2024

Background: Heavy metals are harmful to living organisms and are emitted into the environment from many different sources. Compared to alternative remediation technologies that are either too expensive or impractical, phytoremediation, in which plants are used to remove hazardous toxins from water and soil, is a viable option for use at extremely large field sites. Heavy metals may be removed from the environment by a wide variety of plants. Azolla, a kind of aquatic fern, is one example.Methods: Different concentrations of heavy elements (5,10, and 25mg/ L) were prepared and treated with the plant in different masses (0.2,0.4, and 0.8 grams)and over time periods of one and two weeks. The concentration of each element was measured before and after the treatment periods using an atomic absorption device.Results: As evidenced that spring Azolla (green type) was more effective at removing cadmium and lead elements than autumn Azolla (red type), where the spring Azolla recorded higher removal efficiency for Cd , where it was 1.80% under biomass 0.8g, contact time 10 days, and at metal concentration 10mg\L ,while the highest percentage removal for Pb , was 40.02% under biomass 0.4g, contact time 15 day and at metal concentration 5mg\L . Azolla has great potential for removing heavy metals from water resources and can be used in heavy metal phytoremediation initiatives in environmental improvement projects.Conclusion: This research suggests using the aquatic macrophyte Azolla, especially spring fern, to treat sewage and household wastewater containing Cadmium and Lead.Keywords: Azolla; Heavy metals; Phytoremediation; Cd; Pb
Photosynthesis microbial desalination cell: Analysis and kinetic study of microbial community contribute to biofilm formation, system performance and bioenergy recovery
Authors: Sadeq Ahmed Mustafa , Ismail Zainab Ziad
Published: November 2024

Background: Detection of bacterial species using 16S rRNA is a popular approach in microbiology. This method focuses on 16S rRNA gene which includes both conserved regions shared among bacterial species and variable regions unique to each species. This study aimed for the first time to apply this technique for identifying and classifying the bacterial species which contribute to the formation of anodic biofilm in a tubular photosynthetic microbial desalination cell (PMDC).Methods: A tubular photosynthesis microbial desalination cell was designed and set up for simultaneous wastewater biotreatment and desalination of seawater associated with clean power generation.16S rRNA sequencing was used for characterization of the dominant microbial strains in the anodic biofilm. The materials involve DNA extracting from bacteria and PCR amplifying for 16S rRNA gene. The kinetic of the bacterial growth in relation to the substrate utilization was studied.Results: The results revealed the identification of 19 new dominant microbial strains; 13 in the initial shallow biofilm and 6 in the developed biofilm. Results of evaluating the PMDC performance demonstrated that maximum removal efficiency of organic content from sewage was 93±3% associated with power generation of 24.3±2.5 mW/m3 and 70±4% desalination efficiency of saline water. Results of the kinetic study of biomass growth demonstrated that among the 5 examined models, Monod and Blackman models significantly fitted the experimental data with determination coefficients (R2) of 0.951 and 0.907, respectively.Conclusion: This study adds to our knowledge of the anode biofilm's involvement in PMDC performance by identifying dominant microbial strains using 16S rRNA sequencing. The findings emphasize microbial contributions to simultaneous treatment of wastewater, desalination of sea water, and electricity generation. This experimental and theoretical investigation paves the way for future breakthroughs in microbial desalination technology, addressing crucial water scarcity issues.Keywords: Photosynthesis microbial desalination cell; Microbial characterization; Biofilm; Bacterial strains; Energy production
Exploring Natural Compounds Targeting the Bacterial SHV Protein to Combat Antibiotic Resistance: A Biocomputational Study
Authors: Anjum Farah , Banjer Hamsa Jameel , Hawsawi Nahed , Alsaeedi Fouzeyyah Ali , Alsaiari Ahad Amer , Alharthi Afaf , Almalki Abdulraheem Ali , Hulbah Maram Jameel , Alharthi Norah , Shafie Alaa , Bakhuraysah Maha
Published: November 2024

Background: Antibiotic-resistant (AR) bacteria are rapidly spreading worldwide, posing a serious threat to antibiotic efficacy. Bacterial infections have emerged as a persistent threat following decades of antibiotic use. Sulfhydryl variable (SHV) is a well-known bacterial enzyme linked to AR. SHV has a high degree of genetic diversity, resulting in the existence of numerous distinct variants. Methods: The PyRx AutoDock VINA was used to conduct in-silico screening of a natural compound library to assess their interaction with the SHV-1 protein. SwissADME web tools were used to predict the physicochemical, drug-likeness, and ADMET properties of the selected compounds.Result: The compounds PSCdb00708, PSCdb00149, PSCdb00698, and PSCdb00175 bind strongly to the SHV-1 protein and interact strongly with the SHV-1 active site residues, as well as having several amino acid residue interactions in common with avibactam. These compounds exhibited higher binding affinity values than avibactam. Furthermore, these compounds demonstrated no violation of drug-likeness.Conclusion: The compounds PSCdb00708, PSCdb00149, PSCdb00698, and PSCdb00175 can be employed as SHV-1 inhibitors in the management of AR. However, experimental validation is required to optimize them as SHV-1 inhibitors.Keywords: Antibiotic-resistant; Antibiotic; Virtual screening; AutoDock; SHV-1 protein
Chemical Profiling and Pharmaceutical and Biological Activities of Methanolic Extract of Citrullus colocynthis L. Seeds Collected from the Arid Zone of Qassim, Saudi Arabia against Aphis craccivora
Authors: Abdallah Emad M. , Ali Ahmed M. H. , Hashem Hanaa Fadl , Mujawah Adil A. , Elsharkawy Eman R.
Published: November 2024

Background: Citrullus colocynthis L. (C. colocynthis) is a medicinal plant with a long history of traditional usage in Saudi Arabia, particularly for the treatment of digestive issues such as indigestion and stomach pain.Methods: C. colocynthis seeds were examined using qualitative and quantitative phytochemical methods. Antioxidant testing was conducted using DPPH assay and the reducing power test. Antibacterial properties were evaluated using the well-diffusion test, minimum inhibitory concentration (MIC) test, and minimum bactericidal concentration (MBC) test. The effectiveness of the extract against Aphis craccivora (A. craccivora) was assessed using a leaf-dip bioassay, measuring generation time (GT), net reproduction rate (R0), intrinsic rate of increase (rm), doubling time (DT), and finite rate of growth (λ).Results: The methanolic extract of C. colocynthis seeds included diverse bioactive compounds, a good quantity of phenolic content (60.45 mg GAE/g) and flavonoid content (46.66 mg/g). The antibacterial tests showed that the extract was effective only against Staphylococcus aureus and Proteus mirabilis. The extract recorded inhibition zones of 14.5 ± 0.7 mm and 10.5 ± 0.5 mm for Staphylococcus aureus and Proteus mirabilis, respectively. Staphylococcus aureus showed the greatest sensitivity, with MIC of 6.25 mg/mL and MBC of 25.0 mg/mL. The extract was found to have a fatal concentration (LC50) of 9.02% and a lethal concentration (LC95) of 20.50% against A. craccivora aphid.Conclusions: The current vitro study on C. colocynthis reported that the seeds are effective as antioxidants, antibacterial agents (only against Gram-positive bacteria), and aphicidal agents against aphids. More investigations are recommended to examine the possible toxicity and biochemical interactions in vivo.Keywords: Plants; Biological activity; In vitro study; Citrullus colocynthis; Aphis craccivora; Aphicidal
Bioinformatic Analysis of Oxygen Sensitivity in Arcobacter bivalviorum's Pyruvate:Ferredoxin Oxidoreductase (PFOR)
Authors: Alqurashi Abdulmajeed
Published: November 2024

Background: Arcobacter bivalviorum is an emerging foodborne pathogen particularly found in shellfish and other bivalves. This bacterium's metabolic versatility is highlighted by its possession of both the Pyruvate Dehydrogenase Complex (PDC) and Pyruvate:Ferredoxin Oxidoreductase (PFOR). The PFOR enzyme is crucial for linking glycolysis to the citric acid cycle. Previous studies of PFOR enzyme reveal its susceptibility to oxygen damage due to its reliance on Fe-S clusters for electron transfer.Methods: The current study employs bioinformatic approaches to explore the oxygen sensitivity of A. bivalviorum's PFOR, comparing it with Desulfovibrio africanus and Moorella thermoacetica. The UniProt database was used to obtain the sequences of PFOR from D. africanus (CAA70873), A. bivalviorum (AXH11209), and M. thermoacetica (Q2RMD6). This comparative analysis sheds light on the structural similarities and differences between the enzymes, providing a deeper understanding of their functional mechanisms.Result: PFOR enzyme is a heterodimer, with its functional subunits containing three [4Fe-4S]²⁺ clusters. Its exposed Fe-S clusters are vulnerable to oxygen and ROS, leading to enzyme inactivation. A comparison of PFOR sequences from D. africanus, M. thermoacetica, and A. bivalviorum reveals a crucial difference: the final 43 residues at the C-terminal of D. africanus are missing in the other two enzymes, depriving the M. thermoacetica and A. bivalviorum enzymes of a self-protective mechanism.Conclusion: Pyruvate:ferredoxin oxidoreductase (PFOR) in A. bivalviorum lacks protection from oxidative damage due to the absence of the final 43 amino acids at the C-terminal.Keywords: PFOR; Homology modeling; oxygen sensitivity; Foodborne pathogen
Neimann-Pick Diseases: Beyond Lipid Accumulation – Genetic, Diagnostics, and Therapeutic Strategies
Authors: Naffadi Hind M
Published: November 2024

Rare genetic disorders are the group of disorders/diseases that occur in such a low count that they are not considered much active market for therapeutics unless encouraged by appropriate incentives and support. They are too rare to be fully investigated and managed by health professionals. Genetic disorders are caused by change in genes and are often serious and complex. Around the world, most affected individuals are found to be children. They can be progressive and might get worse as children grow older. Niemann-Pick diseases (NPD) is one such group of rare genetic diseases that are characterized by unwanted and abnormal accumulation of lipids within the cells. In this review, we discuss the history, genetic basis, clinical manifestations and diagnostic approaches for NPD. We further discuss the pathophysiology of accumulation of lipids and their impact on cellular functions and organ systems and various management strategies.Keywords: Rare disease; Genetic mutation; Lipid metabolism; Cholesterol trafficking; Neurological abnormalities
Evaluating the Antioxidant Capacity, Phenolic Contents, and Anticancer Potential of Caralluma europaea Extracts
Authors: Atrooz Omar M. , Al-Awaida Makarem , Al-Awaida Wajdy , Al-Ameer Hamzeh J. , Uysal Hamdi
Published: November 2024

Background: Caralluma europaea (C. europaea) is a genus of shrubs in the Apocynaceae family, endemic to the Mediterranean region. Assessing the anticancer properties, total phenolic and flavonoid content, and antioxidant activity of different C. europaea extracts were the aim of this study.Methods: To determine antioxidant activity, we used three free radical scavenging assays: DPPH (2,2-Diphenyl-1-picrylhydrazyl), hydrogen peroxide (H2O2), and nitric oxide (NO) assays. Total phenolic and flavonoid contents were measured using colorimetric methods. The anti-important activity was evaluated by MTT assay.Results: Our findings showed that the efficacy of the extract in MCF7 cells decreased in the system containing chloroform, methanol, ethyl acetate, and water. The strength of the extract for U87 cells decreased according to methanolic, chloroform and water, where ethyl acetate was the strongest. The highest total phenolic and flavonoid content was found in methanol extract. All extracts showed a high scavenging activity of DPPH. Methanol extract showed the highest antioxidant activity in the NO assay of the solution, followed by ethyl acetate, chloroform, and aqueous extract. In the H2O2-based antioxidant activity assay, the methanol extract again showed the highest activity, followed by ethyl acetate, chloroform, and aqueous extracts.Conclusions: Extracts of C. europaea have  shown great potential as natural antioxidants and anticancer agents in this study, suggesting that they deserve further investigation into the food and pharmaceutical fields.Keywords: Phenolic content; Flavonoid content; Antioxidant activity; Anti-cancer activity
In-Silico Perspectives on the Potential Therapeutic Aids of Hesperetin Derivatives for Lung Cancer
Authors: Asiri Abdulaziz
Published: November 2024

Background: Lung carcinoma has become one of the most noteworthy and dangerous health problems discovered today. The disease is mainly caused by smoking and becomes among the leading causes of death spread by growing the cancerous cells into the lining of the lungs and nearby lobes. The FDA has given clearance to numerous drugs and chemotherapy agents; nevertheless, they can be exceedingly costly as well as frequently fall short of entirely addressing the ailment. In the medical management of lung cancer, new medicines or active leads with high efficacy and minimal toxicity are required in this era.Methods: The study has been conducted with the intent to uncover a prospective approach to treating lung cancer through structural modification of Hesperetin by creating its analogs to enhance its efficacy compared to its parent compound with the computational drug design. The analysis has been conducted with various approaches followed by PASS prediction, ADME, toxicity profile, molecular docking, data filtration, and anticancer activity.Results: All the compounds showed satisfactory criteria in each parameter that was assessed. The data mining was done carefully by pointing out the compounds that had the greatest value among all the compounds in each investigation, which resulted in a total of 3 compounds out of 50. Conclusions: Finally, the selected compounds were further analyzed for MD simulation studies. Afterward, PCA analysis was also conducted in order to get the lead compound with this additional investigation.Keywords: Lung cancer; Anti-cancer lead; Hesperetin; Drug designing; Molecular docking; MD simulation; PCA
Advancing Frontiers in Colorectal Cancer: Exploring Novel Insights and Emerging Trends in Research and Therapy
Authors: Al Ali Amer
Published: November 2024

Colorectal cancer, a known form of cancer poses a global health threat. Ongoing studies are actively investigating perspectives and evolving treatments. This review explores the developments in cancer research covering topics such as profiling, microbiome interactions, immunotherapy, precision medicine, tumor environment analysis, liquid biopsies, metabolic changes, artificial intelligence applications and patient centric care. Molecular subtyping advancements have greatly improved our understanding of the nature of cancer and how different individuals respond to therapies. The significant link between gut microbiome composition, inflammation levels and immune responses underscores the importance of interventions targeting the microbiome and innovative immunotherapy strategies. Precision medicine initiatives are leveraging biomarkers and molecular signals to inform treatment decisions and optimize outcomes. This thorough examination highlights the impact of evolving trends in cancer research and treatment strategies while offering insights into directions for improving patient care and results.Keywords: Cancer research; Microbiome interaction; Immunotherapy; Liquid biopsy; Biomarkers
Veterinary and sanitary assessment of milk quality in black-and-white cows fed with extruded compound feeds in North Kazakhstan
Authors: Zhaxalykov R.A. , Bayazitova K.N. , Polyak A.I. , Kassymbekova L.N. , Mustafina R. , Zabolotnykh M.V.
Published: November 2024

Background: Today, one of the most effective ways to increase the digestibility of nutrients is extrusion processing. Raw materials are exposed to high temperatures and pressure, which leads to qualitative and nutritional transformations. The study aims to identify the effect of extruded compound feeds on cow productivity and the qualitative composition of milk.Methods: Based on the chemical analysis of milk, the following indicators were studied: fat mass fraction, protein mass fraction, casein, lactose, dry matter, skimmed milk residue, urea, density, titrated acidity, active acidity, and somatic cells. An organoleptic evaluation of milk was carried out according to the following indicators: color, smell, taste, appearance, and consistency. Once a month, a control milking was carried out to account for productivity.Results: The EG's raw milk had higher casein and lactose content compared to the CG, with EG II showing the highest levels. Additionally, milk from the EG contained more solids, indicating better nutritional and technological properties, with solid content being 3.8-5.7% higher than in the CG.Conclusion: Based on the obtained results, it was found that milk productivity had increased by 10-20%, the fat mass fraction by 7-10%, the protein mass fraction by 5.1-6.5%, the amount of dry matter by 3.8-5.7%, and lactose by 1.1-4.3%.Keywords: Extruded Compound Feeds; Milk Productivity; Black-And-White Breed; Fat; Protein