bluuebunny/crossref_metadata_2025 huggingface dataset:
Alternative splicing profiling provides insights into the molecular mechanisms of peanut peg development
Authors: Zhao Xiaobo , Li Chunjuan , Zhang Hao , Yan Caixia , Sun Quanxi , Wang Juan , Yuan Cuiling , Shan Shihua
Published: October 2020

Background

The cultivated peanut (Arachis hypogaea) is one of the most important oilseed crops worldwide, and the generation of pegs and formation of subterranean pods are essential processes in peanut reproductive development. However, little information has been reported about alternative splicing (AS) in peanut peg formation and development.

Results

Herein, we presented a comprehensive full-length (FL) transcriptome profiling of AS isoforms during peanut peg and early pod development. We identified 1448, 1102, 832, and 902 specific spliced transcripts in aerial pegs, subterranean pegs, subterranean unswollen pegs, and early swelling pods, respectively. A total of 184 spliced transcripts related to gravity stimulation, light and mechanical response, hormone mediated signaling pathways, and calcium-dependent proteins were identified as possibly involved in peanut peg development. For aerial pegs, spliced transcripts we got were mainly involved in gravity stimulation and cell wall morphogenetic processes. The genes undergoing AS in subterranean peg were possibly involved in gravity stimulation, cell wall morphogenetic processes, and abiotic response. For subterranean unswollen pegs, spliced transcripts were predominantly related to the embryo development and root formation. The genes undergoing splice in early swelling pods were mainly related to ovule development, root hair cells enlargement, root apex division, and seed germination.

Conclusion

This study provides evidence that multiple genes are related to gravity stimulation, light and mechanical response, hormone mediated signaling pathways, and calcium-dependent proteins undergoing AS express development-specific spliced isoforms or exhibit an obvious isoform switch during the peanut peg development. AS isoforms in subterranean pegs and pods provides valuable sources to further understand post-transcriptional regulatory mechanisms of AS in the generation of pegs and formation of subterranean pods.
Circular RNA hsa_circ_0000282 contributes to osteosarcoma cell proliferation by regulating miR-192/XIAP axis
Authors: Li Houkun , He Limin , Tuo Yuan , Huang Yansheng , Qian Bing
Published: October 2020

Background

Circular RNAs (circRNAs) have emerged as a novel category of non-coding RNA, which exhibit a pivotal effect on regulating gene expression and biological functions, yet how circRNAs function in osteosarcoma (OSA) still demands further investigation. This study aimed at probing into the function of hsa_circ_0000282 in OSA.

Methods

The expressions of circ_0000282 and miR-192 in OSA tissues and cell lines were examined by quantitative real-time polymerase chain reaction (qRT-PCR), and the correlation between the expression level of circ_0000282 and clinicopathological features of OSA patients was analyzed. The expressions of X-linked inhibitor of apoptosis protein (XIAP), B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) in OSA cells were assayed by Western blot. The proliferation and apoptosis of OSA cells were examined by CCK-8, BrdU and flow cytometry, respectively. Bioinformatics analysis, dual-luciferase reporter gene assay and RIP experiments were employed to predict and validate the targeting relationships between circ_0000282 and miR-192, and between miR-192 and XIAP, respectively.

Results

Circ_0000282 was highly expressed in OSA tissues and cell lines, which represented positive correlation with Enneking stage of OSA patients and negative correlation with tumor differentiation degree. In vitro experiments confirmed that overexpression of circ_0000282 markedly facilitated OSA cell proliferation and repressed cancer cell apoptosis in comparison to control group. Besides, knockdown of circ_0000282 repressed OSA cell proliferation and promoted apoptosis. Additionally, the binding relationships between circ_0000282 and miR-192, and between miR-192 and XIAP were validated. Circ_0000282 indirectly up-regulated XIAP expression by adsorbing miR-192, thereby playing a role in promoting cancer in OSA.

Conclusion

Circ_0000282 was a novel oncogenic circRNA in OSA. Circ_0000282/miR-192/XIAP axis regulated OSA cell proliferation apoptosis with competitive endogenous RNA mechanism.
De Clérambault’s syndrome revisited: a case report of Erotomania in a male
Authors: Valadas Maria Teresa Tavares Rodrigues Toma , Bravo Lucilia Eduarda Abrantes
Published: October 2020

Background

Erotomania, also known as “de Clérambault’s Syndrome”, is a psychiatric syndrome characterized by the delusional belief that one is loved by another person of, generally of a higher social status. Erotomania has always been a target of attempts of conceptualization, and the utility of regarding Erotomania as an independent syndrome has been questioned to this day. Erotomania has a much higher prevalence in the female sex, and male Erotomania is a rare and probably underdiagnosed condition. Male Erotomania is only more prevalent in forensic samples, since male sex is one of the risk factors for violent behavior in this disorder. In this article, we aim to describe an uncommon case of Erotomania occurring in a male, hoping to add to literature and to reflect on the implications of the occurrence of Erotomania in men. By discussing the case in light of the different described clinical pictures, proposed diagnostic criteria and classifications, we also aim to contribute to the ongoing attempt to conceptualize this syndrome and to understand the pertinence of considering it an independent nosological entity.

Case presentation

We describe a case of Erotomania in a middle-aged Caucasian Portuguese male, with consecutive erotic delusions, followed by a classic turn to a persecutory delusion. The patient was admitted as an inpatient in a psychiatry unit and he was medicated with risperidone 3 mg and diazepam 3 mg daily. His persecutory delusion remitted a 4 days later, he gained insight and was discharged to follow-up as an outpatient. He retains his erotic delusional beliefs, but these are less intense, and has not presented further aggressive behavior.

Conclusions

We can conclude that it seems reasonable to retain the operative concept of Erotomania as a subtype of Persistent Delusional Disorder/Delusional Disorder, since cases fitting the classical descriptions of the syndrome have been reported, including the presented case. The diagnosis of Erotomania has implications in case management, treatment and prognosis, and missing it, especially in men, may culminate in violent situations that can have legal implications. The developed diagnostic criteria and classifications seem to retain use and should be kept in mind, in the sense that they facilitate making an important diagnosis.
“My work? Well, I live it and breathe it”: The seamless connect between the professional and personal/community self in the Aboriginal and Torres Strait Islander health sector
Authors: Dickson Michelle
Published: October 2020

Background

Australian Aboriginal and Torres Strait Islander health professionals often juggle the challenges of working and living in the same community in ways that are positive for both themselves and their clients. This study specifically examines the strategies Aboriginal and Torres Strait Islander health professionals have developed to enable them to feel empowered by the sense of being always visible or perceived as being always available. Findings provide examples of how participants (Team Members) established a seamless working self, including how they often held different perspectives to many work colleagues, how Team Members were always visible to community and how Team Members were comfortable to be seen as working when not at work.

Methods

This qualitative study engages an Indigenous research methodology and uses an Indigenous method, PhotoYarning, to explore lived experiences of a group (n = 15) of Aboriginal and Torres Strait Islander health workers as they worked in the Australian health sector.

Results

The analysis presented here comes from data generated through PhotoYarning sessions. Team Members in this study all work in health care settings in the communities in which they also live, they manage an extremely complex network of interactions and relationships in their daily working lives. They occupy an ambivalent, and sometimes ambiguous, position as representing both their health profession and their community. This article explores examples of what working with seamlessness involved, with findings citing four main themes: (1) Being fellow members of their cultural community, (2) the feeling of always being visible to community as a health worker, (3) the feeling of always being available as a health worker to community even when not at work and (4) the need to set an example.

Conclusions

While creating the seamlessness of working and living in the same community was not easy, Team Members considered it an important feature of the work they did and vital if they were to be able to provide quality health service to their community. However, they reported that the seamless working self was at odds with the way many of their non-Indigenous Australian colleagues worked and it was not well understood.
Evaluation of an isothermal amplification HPV detection assay for primary cervical cancer screening
Authors: Zhang Wei , Du Hui , Huang Xia , Wang Chun , Duan Xianzhi , Liu Yan , Shi Bin , Zhang Wei , Qu Xinfeng , Wei Lihui , Schiffman M. , Belinson J. L. , Wu Ruifang
Published: October 2020

Objective

The aim of this research was to evaluate independently the performance of a new isothermal amplification assay for cervical cancer screening compared to two previously validated PCR-based assays and histologic endpoints.

Methods

This is a sub-study from the Chinese multi-center screening trial (CHIMUST). The self-collected and clinician-collected specimens stored in PreservCyt at − 4 °C from 6042 women with complete data were tested with the AmpFire assay. These specimens had been previously tested with Cobas and SeqHPV assays. In the primary study all patients with an abnormal test were referred to colposcopy where all had directed and/or random biopsies plus ECC. No additional patients were called back based on the AmpFire results.

Results

6042/6619 women had complete data (mean age 44.1). There were 57 cases of CIN 2, 35 cases of CIN 3 and 2 cancers. The sensitivity for CIN2+ and CIN3+ were similar among the three assays (both direct and self-collected). For the specificities in all categories (CIN2+/CIN3+ and self and direct collection), isothermal amplification assay was either equal to or more specific than Cobas but consistently less specific than SeqHPV.

Conclusion

The AmpFire HPV assay showed similar sensitivity to Cobas and SeqHPV for CIN2+ and CIN3+ on both self and clinician-collections (P>0.05), with good specificity. The speed, low cost, and simplicity of this assay will make it particularly suited for low and middle resource settings. Its accuracy with self-collection makes it applicable for mass screening programs.
Differences and similarities of high-resolution computed tomography features between pneumocystis pneumonia and cytomegalovirus pneumonia in AIDS patients
Authors: Du Chun-Jing , Liu Jing-Yuan , Chen Hui , Yan Shuo , Pu Lin , Xiong Hao-Feng , Xiang Pan , Li Chuan-Sheng , Zhang Ming , Xie Ru-Ming , Chen Bu-Dong , Li Ang
Published: October 2020

Background

Accurately differentiating pneumocystis from cytomegalovirus pneumonia is crucial for correct therapy selection in AIDS patients. Hence, the goal of this study was to compare the computerized tomography (CT) features of pneumocystis pneumonia and cytomegalovirus pneumonia in AIDS patients and identify clinical hallmarks to accurately distinguish these two pathologies.

Methods

A total of 112 AIDS patients (78 with pneumocystis pneumonia and 34 cytomegalovirus pneumonia) at Beijing Ditan Hospital from January 2017 to May 2019 were included in this study. Two experienced chest radiologists retrospectively reviewed CT images for 17 features including ground-glass opacity, consolidation, nodules, and halo sign. Binary logistic regression analyses were conducted to identify the significant parameters that distinguished pneumocystis pneumonia from cytomegalovirus pneumonia. Correlations were analyzed by Pearson or Spearman correlation analyses. Result were considered significant if P < 0.05.

Results

The presence of consolidation, halo signs, and nodules (all P < 0.05) were significantly more frequent in patients with cytomegalovirus pneumonia than in those with pneumocystis pneumonia. Small nodules (32.5% in cytomegalovirus pneumonia, 6.41% in pneumocystis pneumonia, P < 0.001) without perilymphatic distribution were particularly common in patients with cytomegalovirus pneumonia. Large nodules were not found in any of patients with cytomegalovirus pneumonia. The presence of ground-glass opacity, reticulation, and bronchial wall thickening (all P > 0.05) were common in both groups.

Conclusions

Analysis of consolidation, nodules, and halo signs may contribute to the differential diagnosis of pneumocystis pneumonia or cytomegalovirus pneumonia. However, some CT features considered typical in one or other diseases appear with similar frequency in both cohorts of AIDS patients. CT features are potentially useful for the differential diagnosis of pneumocystis pneumonia and cytomegalovirus pneumonia in AIDS patients.
Making the invisible visible: a place for utilizing activity theory within in situ simulation to drive healthcare organizational development?
Authors: Gormley Gerard J. , Kajamaa Anu , Conn Richard L. , O’Hare Sarah
Published: October 2020

Background

The healthcare needs of our societies are continual changing and evolving. In order to meet these needs, healthcare provision has to be dynamic and reactive to provide the highest standards of safe care. Therefore, there is a continual need to generate new evidence and implement it within healthcare contexts. In recent times, in situ simulation has proven to have been an important educational modality to accelerate individuals’ and teams’ skills and adaptability to deliver care in local contexts. However, due to the increasing complexity of healthcare, including in community settings, an expanded theoretical informed view of in situ simulation is needed as a form of education that can drive organizational as well as individual learning.

Main body

Cultural-historical activity theory (CHAT) provides us with analytical tools to recognize and analyse complex health care systems. Making visible the key elements of an in situ simulation process and their interconnections, CHAT facilitates development of a system-level view of needs of change.

Conclusion

In this paper, we theorize how CHAT could help guide in situ simulation processes—to generate greater insights beyond the specific simulation context and bring about meaningful transformation of an organizational activity.
Governance of a Learning Health Care System for Oncology: Patient Recommendations
Authors: Jones Rochelle D. , Krenz Chris , Griffith Kent A. , Spence Rebecca , Bradbury Angela R. , De Vries Raymond , Hawley Sarah T. , Zon Robin , Bolte Sage , Sadeghi Navid , Schilsky Richard L. , Jagsi Reshma
Published: October 2020

PURPOSE:

The learning health care system (LHS) was designed to enable real-time learning and research by harnessing data generated during patients’ clinical encounters. This novel approach begets ethical questions regarding the oversight of users and uses of patient data. Understanding patients’ perspectives is vitally important.

MATERIALS AND METHODS:

We conducted democratic deliberation sessions focused on CancerLinQ, a real-world LHS. Experts presented educational content, and then small group discussions were held to elicit viewpoints. The deliberations centered around whether policies should permit or deny certain users and uses of secondary data. De-identified transcripts of the discussions were examined by using thematic analysis.

RESULTS:

Analysis identified two thematic clusters: expectations and concerns, which seemed to inform LHS governance recommendations. Participants expected to benefit from the LHS through the advancement of medical knowledge, which they hoped would improve treatments and the quality of their care. They were concerned that profit-driven users might manipulate the data in ways that could burden or exploit patients, hinder medical decisions, or compromise patient-provider communication. It was recommended that restricted access, user fees, and penalties should be imposed to prevent users, especially for-profit entities, from misusing data. Another suggestion was that patients should be notified of potential ethical issues and included on diverse, unbiased governing boards.

CONCLUSION:

If patients are to trust and support LHS endeavors, their concerns about for-profit users must be addressed. The ethical implementation of such systems should consist of patient representation on governing boards, transparency, and strict oversight of for-profit users.
Antibiotics in hives and their effects on honey bee physiology and behavioral development
Authors: Ortiz-Alvarado Yarira , Clark David R. , Vega-Melendez Carlos J. , Flores-Cruz Zomary , Domingez-Bello Maria G. , Giray Tugrul
Published: October 2020

Recurrent honey bee losses make it critical to understand the impact of human interventions, such as antibiotics use in apiculture. Antibiotics are used to prevent or treat bacterial infections in colonies. However, little is known about their effects on honey bee development. We studied the effect of two commercial beekeeping antibiotics on the bee physiology and behavior throughout development. Our results show that antibiotic treatments have an effect on amount of lipids and rate of behavioral development. Lipid amount in treated bees was higher than those not treated. Also, the timing of antibiotic treatment had distinct effects for the age of onset of behaviors starting with cleaning, then nursing and lastly foraging. Bees treated during larva-pupa stages demonstrated an accelerated behavioral development and loss of lipids, while bees treated from larva to adulthood had a delay in behavioral development and loss of lipids. The effects were shared across the two antibiotics tested, TerramycinR (oxytetracycline) and TylanR (tylosin tartrate). These results on effects of antibiotic treatments suggest a role of microbiota in the interaction between the fat body and brain that is important for honey bee behavioral development.
Mesoporous Alumina-Titania Composites with Enhanced Molybdenum Adsorption towards Medical Radioisotope Production
Authors: Benu Didi Prasetyo , Earnshaw Jacob , Ashok Aditya , Tsuchiya Kunihiko , Saptiama Indra , Yuliarto Brian , Suendo Veinardi , Mukti Rino Rakhmata , Fukumitsu Nobuyoshi , Ariga Katsuhiko , Kaneti Yusuf Valentino , Yamauchi Yusuke
Published: October 2020

This work reports the fabrication of mesoporous Al2O3-TiO2 composites with various contents of TiO2 and their utilization as adsorbents for molybdenum anions with potential application in medical radioisotope production. The increase of TiO2 promotes the deposition of more TiO2 nanoparticles on the surface of the Al2O3 support without altering the original morphology. Furthermore, alumina samples loaded with smaller amounts of TiO2 (Al2O3-2.5% Ti (20.01 mass% TiO2) and Al2O3-5% Ti (34.55 mass% TiO2)) are amorphous in nature. However, at a high loading (Al2O3-10% Ti (63.97 mass% TiO2)), anatase TiO2 becomes the dominant phase, suggesting the extensive coverage of the Al2O3 surface by TiO2 NPs. The TiO2 modification is also observed to greatly increase the surface area from 177 m2 g−1 for pristine γ-Al2O3 to as high as 982 m2 g−1 for Al2O3-5% Ti sample. When employed for molybdenum adsorption, the Al2O3-5% Ti sample displays a higher Mo adsorption capacity (44.5 mg g−1) than Al2O3-2.5%Ti (39.0 mg g−1), Al2O3-10%Ti, (40.5 mg g−1), and pristine γ-Al2O3 (37.1 mg g−1) samples. The larger surface area and presence of additional hydroxyl groups for attracting the molybdenum anions contribute to the enhanced adsorption capacities of the Al2O3-TiO2 composites compared to that of pristine γ-Al2O3.
The Potential Health Benefits of Meditation
Authors: Allen Cynthia
Published: October 2020

Apply It!

By reading this article, health and fitness professionals will:

• Understand what meditation is and the multiple forms in which it is taught and practiced,

• Identify the potential health benefits of consistent meditation training, and

• Explore strategies for developing a regular meditation practice.
Medical and surgical devices in the emergency and trauma patient: what the radiologist should know, and how they can add value
Authors: De La Hoz Polo Marcela , Sandhu Amandeep , Kashef Elika , Aylwin Christopher , Bew Duncan , Manikon Maribel , Dick Elizabeth
Published: October 2020

A variety of different external and internal medical devices are used in the acute setting to maintain life support and manage severely injured and unstable trauma or emergency patients. These devices are inserted into the acutely ill patient with the specific purpose of improving outcome, but misplacement can cause additional morbidity and mortality. Consequently, meaningful interpretation of the position of devices can affect acute management. Some devices such as nasopharyngeal, nasogastric and endotracheal tubes and chest and surgical drains are well known to most clinicians, however, little formal training exists for radiologists in composing their report on the imaging of these devices. The novice radiologist often relies on tips and phrases handed down in an aural tradition or resorts to phrases such as: “position as shown”. Furthermore, radiologists with limited experience in trauma might not be familiar with the radiological appearance of other more specific devices. This review will focus on the most common medical devices used in acute trauma patients, indications, radiological appearance and their correct and suboptimal positioning.
A EXCLUSÃO E SEUS DESDOBRAMENTOS ORIUNDO DE UMA PANDEMIA: REFLEXÕES A PARTIR DO MOVIMENTO POR UMA ESCOLA INCLUSIVA PARA ESTUDANTES COM DEFICIÊNCIA
Authors: Redig Annie Gomes , Mascaro Cristina Angélica Aquino de Carvalho
Published: October 2020

O artigo discute a inclusão à luz dos sentimentos de isolamento e exclusão advindos da pandemia do COVID-19. Objetivou-se refletir sobre o relato de jovens com transtorno do espectro autista de como vivenciam e lidam com a escola em momentos de isolamento. A análise permitiu concluir a necessidade de mudanças na formação inicial e continuada de professores na perspectiva da Educação Inclusiva. Constatou-se a ausência de suportes adequados para os professores, neste momento de isolamento social, para atender aos estudantes com deficiência, ainda que a intenção dos sistemas de ensino tenha sido de incluir os educandos por meio de atividade nas mídias digitais. Considera-se a que a dificuldade de inclusão no período de pandemia desvela a situação de exclusão já vivenciada por este público.
ENSINO SUPERIOR E PRODUTIVIDADE ACADÊMICA EM TEMPOS DE PANDEMIA
Authors: Moura Aline De Carvalho , Cruz Andreia Gomes da
Published: October 2020

Por meio de levantamento bibliográfico e de análise documental, este artigo tem como objetivo problematizar as ações do ensino superior diante do cenário de Covid-19 e seus efeitos no incremento da produtividade acadêmica, no contexto brasileiro. Diante de uma pandemia de escala global, os Institutos de Ensino Superior têm demostrado sua ‘vocação pública’ em diversas frente de ações em busca de um entendimento sobre a doença e seus impactos no Brasil e no sistema público de saúde, o que vem promovendo alta produtividade em um movimento de fast science sobre o Coronavírus. Como considerações, criticamos uma naturalização das pressões exercidas pela produtividade em meio à crise sanitária planetária devido a Covid-19.
Impactos do Covid-19: as visões e experiências de crianças e jovens portugueses em situação de acolhimento residencial
Authors: Valasques Daiane , Santos Hugo
Published: October 2020

A pandemia veio a questionar as estruturas mais fundacionais das nossas sociedades globais, incluindo o direito democrático à educação. A partir de uma abordagem participatória, envolvendo photovoiced, entrevistas e grupos focais, quis-se perceber quais os impactos psicológicos e educacionais da pandemia nos quotidianos de crianças e jovens em situação de acolhimento residencial. Os resultados indicam que as crianças e jovens se sentem isolados e entediados, chegando a ser difícil cumprir as regras para certos grupos. Esse stress estende-se às suas experiências educacionais que somadas à falta de recursos tecnológicos e técnicos da instituição, torna o direito a ser educado mais difícil. Chama-se a atenção para a necessidade de investir educacionalmente nestas instituições.
AMBIÊNCIAS FORMATIVAS EM TEMPO DE NOVAS EDUCAÇÕES: O QUE APRENDEMOSENSINAMOS COM A PANDEMIA
Authors: De Castro Luis Henrique Monteiro , Dos Santos Rosemary
Published: October 2020

O presente artigo se propõe a investigar e mapear algumas práticas de aprendizagemensino com o uso da mediação tecnológica em rede no cotidiano de um grupo de alunos da disciplina Eletrônica Digital de uma Escola Técnica na Zona Norte do Rio de Janeiro durante um período da pandemia do COVID-19. As atividades foram desenvolvidas através do uso de um Ambiente Virtual de Aprendizagem (AVA) baseado no Moodle e de interfaces de web conferência. Dessa forma, procuramos problematizar algumas questões a respeito da cultura contemporânea, como rede educativa.  Ao trazermos como dispositivos de pesquisa o WhatsApp e o Moodle encontramos nas conversas a possibilidade de articulação e ressignificação de novas práticas cotidianas nos espaços e nos tempos escolares.
The Institute for Health Protection and its role in tackling COVID-19
Authors: Glasper Alan
Published: October 2020

Emeritus Professor Alan Glasper, from the University of Southampton, discusses the role of the newly created Institute for Health Protection, which has subsumed the work of Public Health England and other organisations
We can't leave social care in the cold
Authors: Foster Sam
Published: October 2020

Sam Foster, Chief Nurse, Oxford University Hospitals, considers the challenges facing the social care sector and the need for more joined-up working between health and social care
Sarcoidosis: a neglected inflammatory disease?
Authors: Booth Helen
Published: October 2020

New clinical practice guidelines for sarcoidosis provide recommendations to address uncertainties commonly confronted by clinicians, but highlight the need for good quality clinical research for this ‘neglected’ inflammatory disease.
Effects of 4 Thiopurine Compounds on Nitric Oxide Production and Cell Viability of HIG-82 Synoviocytes and RAW 264.7 Macrophages
Authors: C. K Dahlan-Daud , Z. N Zain , C. L. Tham , Y. K. Yong , M. N Hakim
Published: October 2020

The 6-Mercaptopurine (6-MP) is a purine sulphur derivative used in the treatment of children with leukaemia as an antitumor medication. Nonetheless, the anti-proliferation and anti-rheumatoid arthritic activities of the drug have not been previously described. Therefore, the current study objective was to determine the cytotoxicity effects of 6-MP, 6-mercaptopurine riboside (6-MPR), 6-thioguanine (6-TG) and 6-thioxanthine (6-TX) on murine macrophages (RAW 264.7) and rabbit synoviocytes (HIG-82) cell lines. An inflammatory stage of RAW 264.7 and HIG-82 cell lines stimulated with Escherichia coli lipopolysaccharide (LPS) and phorbol-12-myristate acetate (PMA), respectively were treated with the drugs at a serials concentration 3.125 – 100 µM. Subsequently, the MTT assay was used to determine the viability of cells. The inhibitory effects were measured based on nitric accumulation in the conditioned media. The results showed that all drugs tested did not show any cytotoxic effect on both cell lines at low and medium concentrations, which the HIG-82 cell viability was more than 80%. However, the drugs display anti-proliferation property on RAW 264.7 cells compared to control. Reduction on cell proliferation was found on all tested drugs. Among its, the diclofenac significantly reduced the proliferation on HIG-82 cell compared to other drug compounds. Inhibitory effects of compound on nitric oxide production PMA-stimulated HIG-82 had only a small inductive effect, but excellent inhibition was observed on RAW 264.7 cell. Meanwhile, 100 μM 6-thioguanine and diclofenac had cytotoxic effect to RAW 264.7 and HIG-82 cell respectively. All thiopurines enhanced the proliferation of HIG-82 and RAW 264.7 cells with no cytotoxic effects. This finding opens new avenues for treating RA and the anti-rheumatic activities during synovial inflammation stage of RA. The inhibitory effects of 6-MP and 6-MPR to inflammatory cells marker such as synovial fibroblast and macrophages by proliferating healthy synoviocytes more potenly than 6-TG and 6-TX. The current study has demonstrated the potential use of 6-MP and its associated thio compounds in the in-vitro approach of HIG-82 cell culture in curing rheumatoid arthritis disease.
APPLICATION OF THE CONSTRUCTION WASTE AS A REDUCING AGENT IN THE SOLUBILITY OF IRON TAILING METALS
Authors: Almeida Sarah Moreira de , Matos Antonio Teixeira de , Puiatti Gustavo Alves , Barros Raphael Tobias Vasconcelos de
Published: October 2020

Iron ore tailings has acid pH and high content of metals, such as iron, aluminum and manganese. Regarding the environment, these metals poses a risk of contaminating the soil and underground water. Yet, construction residues usually have high pH and because of this, they can be used to neutralize soil acidity and to reduce metal availability in mining tailing piles. The objective of this study was to evaluate the aplicabilith of CW to promote neutralization of the acidity in iron ore tailings aiming at decreasing the solubility and mobilith of metal found in them. Thus, a sample of construction waste was collected and characterized which was later incorporated at different doses into a sample of iron mining tailing collected in the Iron Quadrangle (Minas Gerais – Brazil). The construction waste raised the pH of the tailing sample from 5.58 to 8.22. Moreover, the incorporation of the waste incorporation decreased the manganese solubility in at least 80%. Despite the lack of national legislations and policies on the use of construction waste for this purpose, the results show that it is a promising technological alternative.
Teachers' attitudes towards regional dialects in Hungary
Authors: Parapatics Andrea
Published: October 2020

A la mayoría de los húngaros se les enseña durante la educación obligatoria contenido de tipo sociolingüístico sobre el valor de la diversidad lingüística, especialmente de las variedades regionales. Sin embargo, perviven las creencias estereotipadas sobre los hablantes de variedades dialectales que, junto con la cultura basada en estándares, pueden ser la base de debates sobre prejuicios en la comunicación diaria o de la discriminación lingüística. Este estudio busca la explicación del problema investigando las actitudes y prácticas lingüísticas que tienen los docentes en relación con las variedades lingüísticas de sus alumnos. La hipótesis principal es que, si bien el Currículo Nacional promueve una actitud de tolerancia, la práctica prescriptiva sugiere que esas actitudes existen solo en la teoría y que las lecciones sobre la variedad dialectal generan prejuicios en muchos hablantes de la comunidad húngara - incluso entre los jóvenes. Aunque se enseña a los estudiantes a estar orgullosos de los dialectos de sus regiones de origen, la insuficiente información sobre la variedad dialectal les lleva a tener problemas de aceptación, a pesar de que el estudio de esos antecedentes regionales podría ser una ventaja para desarrollar una conciencia metalingüística. Enfatizamos que esta ambivalencia está enraizada en la falta de formación docente en la Educación Superior - en relación con la variación lingüística y con la falta de recursos metodológicos, por lo que este estudio también recomienda algunos materiales educativos recientes sobre el tema.
Auxiliary interferometer in an optoelectronic swept-frequency laser and its application to the measurement of the group refractive index
Authors: Zhao Enming , Shen Heliang , Liu Shuangqiang , Liu Guangyu , Zhou Bao , Wang Chen , Xing Chuanxi , Miao Peixian , Shi Yanchao
Published: October 2020

An optoelectronic swept-frequency laser (SFL) is an optoelectronic feedback system that includes an auxiliary interferometer that can exert precise control over the optical frequency sweep. The arm-length difference (ALD) of the auxiliary interferometer directly affects the performance of the whole system. We established a theoretical model to choose the optimal ALD of an auxiliary interferometer in an optoelectronic SFL system using a frequency-modulated continuous-wave reflectometry experimental setup. The experimental results indicated that, based on our system, the optimal ALD was 7 m, which agreed with the theoretical analysis. As an example application, we implemented the proposed system for measurement of the group refractive index of a glass sample. A minimum measurement error of 0.12% was obtained with the ALD of 7 m.
Self-Medication Practices among Medical and Non-Medical Students
Authors: Anwar Isha , Minimol Krishna , Narasimhaiah Manjunath
Published: October 2020

BACKGROUND Self-medication practice involves consumption of medicines by one’s own initiative or on the consultation of others without the guidance of a doctor. Self-medication and use of over-the-counter drugs, are worldwide health concerns. This study evaluated the prevalence, behavioural patterns, knowledge of self-medication, and the attitudes toward this practice among medical and nonmedical university students. METHODS This is a cross-sectional descriptive study conducted among students with medical and non-medical backgrounds. A semi-structured questionnaire was constructed after reviewing literature to capture data regarding socio-demographic background, practice and knowledge of self-medication. RESULTS Out of a total of 98 students, 79.6 % of students practiced self-medication (medical - 77.5 % and non-medical - 85.2 %). The main reason for self-medication was convenience (50.8 %) and the choice of self-medication was based on their own experience (30.4 %) and previous doctors’ prescription (20.2 %). Community pharmacies (64.0 %) were the most common source of acquiring the drugs in both groups. It was observed that 53.1 % students think self-medication is an acceptable practice. 28.6% were confident of treating infectious diseases through selfmedication. CONCLUSIONS The present study shows a higher prevalence of self-medication among students. It also indicates a critical need for the implementation of quality education programs in order to prevent the self-medication among students. KEY WORDS Self-Medication, Medical, Non-Medical
Book Review: Media and Moral Education: A Philosophy of Critical Engagement
Authors: Brown Cheryl L.
Published: October 2020

Media and moral education tackles the topic of how we educate young people to live and learn in a digital world through the adoption of a moral standpoint. This is a particularly pertinent issue for educators as the digital world is fraught with pitfalls and challenges and in many contexts educators and educational institutions fall short of dealing with the complexities of digital literacy and digital citizenship directly. We have moved beyond the issue of accessibility to information and media, to the need to focus on the ability to seek, analyse and critically evaluate and apply information and media content to purposeful decision-making and choices, particularly in relation to learning (Grizzle and Calvo, 2013). With technology being increasingly ubiquitous and pervasive we need to move our attention beyond the mechanics of access and ability into the implications of living digitally.
The use of vortioxetine for depression in patients with Parkinson's disease in the early and advanced stages of the disease
Authors: Miliukhina I. V.
Published: October 2020

Depression is one of the most common non-motor manifestations of Parkinson's disease (PD), which largely determines both the severity of the course of the disease and the life expectancy of patients, as well as the compliance of patients to and the efficiency of antiparkinsonian therapy. In this connection, the search for a safe and effective antidepressant for patients with PD is of particular relevance.

Objective: to evaluate the efficacy and safety of vortioxetine in patients with PD complicated by mild to moderate depression.

Patients and methods. Examinations were made in 150 patients with PD in its early and advanced stages (Hoehn-Yahr stages: 1.0 to 3.0). All the patients were treated with vortioxetine at a dose of 15 or 20 mg/day for 8 months. The investigators used clinical and psychopathological rating scales, such as the Hospital Depression Scale (HADS-D), the Hospital Anxiety Scale (HADS-A), the Beck Depression Inventory (BDI), and the Montgomery-Asberg Depression Rating Scale (MADRS). The severity of PD motor manifestations was assessed according to the Unified PD Rating Scale (UPDRS) Part III.

Results and discussion. During the follow-up period corresponding to 12 weeks of vortioxetine use, all the patients showed a significant decrease in MARDS, HADS-A and HADS-D, and BDI scores for depression and anxiety (p < 0.001). Vortioxetine demonstrated an optimal balance between tolerability and clinical efficacy in correcting affective disorders in this patient group. In addition, analysis of the dynamics of motor disorders yielded data on the improvement of movement functions while correcting depression as lower UPDRS Part III total scores (p < 0.001).

Conclusion. The findings suggest that vortioxetine has a significant effect on depression and anxiety in patients with PD in its early and advanced stages, good tolerability, and a rapid-onset effect.
« Tu es devenu toubab »
Authors: Sambou Césarine
Published: October 2020

La migration et le retour temporaire dans la société de départ sont des moments importants, tant pour le migrant que pour sa famille restée au pays. Les proches non migrants attendent beaucoup du migrant qui revient en termes de solidarité et de redistribution des richesses accumulées en Europe. À partir d’une enquête qualitative multisituée à Bordeaux auprès de migrants originaires d’Afrique subsaharienne et de leurs familles au Sénégal et au Burkina Faso, cette étude présente les enjeux du retour temporaire au prisme des attentes de la famille non migrante. Les résultats montrent comment les migrants originaires d’Afrique subsaharienne, qui rencontrent des difficultés à répondre aux attentes de la famille non migrante, sont étiquetés de « toubab », et les effets de cet étiquetage sur le plan social, familial et identitaire.
Design of Powernet Knitted Fabrics from PET/BaTiO3 as Pressure Garments for Rehabilitation of Cerebral Palsy
Authors: Varan Nilüfer Yıldız
Published: October 2020

In this study, powernet warp-knit fabrics were designed using polyethylene terephthalate (PET) yarns melt-spun with three different amounts (20, 25, and 30 wt.%) of barium titanate (BaTiO3) and 30 wt.% elastane yarns. The resulting fabrics were characterized by antimicrobial activity cytotoxicity electromagnetic shielding properties, differential scanning calorimetry (DSC) analyses, stiffness tests at MD and CD (machine and cross directions), and pressure measurements using wireless pressure sensors. According to the results, the newly-designed pressure garments would help in the rehabilitation of cerebral palsy patients to improve motor skills and to prevent complications by training muscles with the needed tight structure and electric stimulus onto limbs. They would also provide a hygienic environment during long physical therapy for future designs.
Green Reduction of Graphene Oxide Coated Polyamide Fabric Using Carob Extract
Authors: Gültekin Nergis , Usta İsmail , Yalçin Bahattin
Published: October 2020

A green reduction processes for graphene oxide using carob extract is reported in this work. In this study, graphene oxide (GO) nanosheets were synthesized using the improved Hummer's method and applied to polyamide fabric thorough the simple dip coating method. Then, the graphene oxide was reduced with a chemical reduction process using carob extract as a green reducing agent to give the reduced graphene oxide (RGO) material. The reduction time was studied. The structure, morphology, and thermal behavior of the material was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and thermogravimetric analysis (TGA), respectively. The electrical resistivity results clearly revealed that the GO coated polyamide fabric was successfully converted to the RGO coated polyamide fabric with the effective elimination of oxygen containing functional groups.
Marcin syn Pawła z Gołocic (zm. 1448) – wikariusz katedry i notariusz kapituły gnieźnieńskiej, archidiakon i oficjał pomorski, skryptor kanonu mszalnego
Authors: Czyżak Marta
Published: October 2020

The article presents the person of Marcin son of Paweł of Gołocice, descendant of the minor gentry of Łęczyca. The author describes his career as notary (notary imperiali auctoritate, notary of the Gniezno consistory and chapter, surrogate of the Gniezno official and official of Pomerania) and beneficiary (parson of Borkowo, Sompolno and Staw, vicar of the Gniezno cathedral, Pomeranian archdeacon in the Włocławek chapter). On the one hand, emphasis is placed on Marcin’s personal talents which enabled him to perform various offices and functions in the service of the Gniezno college of vicars, cathedral and chapter (watchmaker, succentor of the cathedral choir, prefect of the construction of the cathedral). On the other hand, attention is drawn to the patronage of Władysław Oporowski, a Gniezno canon and bishop of Włocławek (1434-1449), thanks to whom Marcin of Gołocice, as one of three out of 106 vicars of the Gniezno cathedral, in the first half of the 15th century obtained prelature of the cathedral chapter and was nominated Pomeranian archdeacon in the Włocławek chapter.  Marcin of Gołocice is also the only known vicar-scriptor, the author of the inscription of the canon of the Holy Mass with a detailed Polish gloss in a manuscript which is now property of the National Library of Poland.
Studnia przydrożna z XVI-XVII wieku na Ostrowie Tumskim w Poznaniu
Authors: Antowska-Gorączniak Olga , Lech Paweł , Sikorski Andrzej
Published: October 2020

In July 2008 a roadside well was discovered on the cathedral island in Poznań (to date at least five drawing wells from the late Middle Ages and modern times have been registered).  The well was unearthed at the rose square (excavation pit 51/2008) about 18 m from the facade of the church of the Blessed Virgin Mary (and the now nonexistent churchyard) at the level of  56.09 meters above sea level (rubble fill-in) – 50.61 meters above sea level (end of exploration) – Fig. 1.  The object was situated directly next to the road, in a large oval or quadrilateral pit (with rounded corners) ca 3.6 m in diameter, and was ca 5.5 m deep. The wooden construction which protected the walls of the object was done in the post and beam technique – Fig. 2. Many different objects fell (were thrown) into the well while water was being drawn from it, perhaps some of them were redeemed while other remained at the bottom. However, most of the “sunk” relics got into the well while it was being dug or repaired, but especially when it was filled in, i.e. when water was no longer drawn from it (Figs. 4 and 5).The well, which was sunk during the rule of Bishop Jan Lubrański (1499-1520), i.e. at a time of prosperity of Ostrów Tumski in the 16th century, was in use and water was drawn from it in the 15/16-17th centuries. It is difficult to assert what reasons decided about its being
„Szkółka Niedzielna” pod redakcją ks. Tomasza Borowicza (1837-1849) i Ignacego Koteckiego (1849-1853)
Authors: Biesiada Jacek
Published: October 2020

“Szkółka Niedzielna” (“The Sunday School”), a weekly published in Leszno from 1837 to 1853, was the first Polish journal for the peasantry. It was established on Dezydery Chłapowski’s initiative by Fr. Tomasz Borowicz, who was its first editor until mid-1849 (until the end of 1853 this function was performed by Ignacy Kotecki). Articles which appeared in the weekly not only urged readers to pursue school education but in themselves were a source of knowledge on religion, farming, horticulture, animal husbandry and veterinary medicine, also offering guidance on broadly understood country life (including among others such issues as household management, childcare, personal hygiene). Considerable space was allotted to anti-alcoholic texts. Financial difficulties caused the decline of “Szkółka Niedzielna”.
Akademia Lubrańskiego w Poznaniu. Próba wskrzeszenia (1937)
Authors: Szczepaniak Maciej
Published: October 2020

In the period of the Second Republic of Poland two attempts – unfortunately unsuccessful – were undertaken to organize the teaching of theology at university level in Poznań. Endeavors to create a Theological Faculty at Poznań University in the years 1919-1921 did not bring the expected effects. In 1937 Cardinal August Hlond addressed a plea to the Apostolic See to reinstate the Lubrański Academy in Poznań. The Primate’s vision included the construction of a modern building of the academy which he saw as his personal gift to the archdiocese and a “remembrance” of his primateship.The article takes up the above mentioned issue in the light of the Vatican’s archival materials. They allow us to grasp some essential historical facts and provide access to the drafts of the statutes of the intended reactivated Academy. Their publication herein seems to be an interesting contribution to the outlining of a complete history of the Faculty of Theology in Poznań.
„Bądź pozdrowion Krzyżu Pana Wszechmocnego”. Kafel z badań archeologicznych przy ul. Za Bramką w Poznaniu
Authors: Zisopulu-Bleja Kateriny
Published: October 2020

Przedmiotem artykułu jest kafel z przedstawieniem Grupy Ukrzyżowania odkryty podczas badań archeologicznych w fosie miejskiej przy ul. Za Bramką w Poznaniu. Kafle były wytworem rzemiosła garncarskiego, natomiast ich ornamentyka podlegała zmienności związanej z aktualnie panującymi trendami i stylami zdobniczymi. Omawiany zabytek datowany jest na początek XVI wieku, jego dekoracja wykonana została w oparciu o gotyckie wzory graficzne i symbolikę charakterystyczną dla sztuki pasyjnej tego okresu. Miejsce znalezienia kafla może wskazywać na fundatora pieca wywodzącego się z kręgu osób duchownych zamieszkujących w bezpośrednim sąsiedztwie kolegiaty św. Marii Magdaleny.
Pobór kleryków do wojska jako forma represji władz komunistycznych wobec Kościoła katolickiego. Alumni-żołnierze archidiecezji poznańskiej
Authors: Wilczyński Leszek
Published: October 2020

Po raz pierwszy poboru kleryków do służby w Ludowym Wojsku Polskim dokonano w 1955 roku. Była to nowa metoda represji zastosowana wobec Kościoła katolickiego, zaś jej celem było zniechęcenie kandydatów do kapłaństwa do realizacji obranej drogi życiowej. Kres tym prześladowaniom położył dopiero wybór papieża-Polaka na Stolicę Piotrową. Ogółem w latach 1955-1979 represjonowano 2.713 kleryków w całej Polsce.Artykuł omawia tło historyczne, motywacje władz komunistycznych, przeciwdziałanie ze strony Episkopatu Polski i poszczególnych hierarchów, akcentując w sposób szczególny działania ochronne podejmowane przez duchowieństwo archidiecezji poznańskiej wobec młodzieży kleryckiej służącej w Ludowym Wojsku Polskim. Podstawą źródłową była korespondencja kleryków z przełożonymi seminarium oraz opracowania dotyczące najnowszej historii państwa i Kościoła.