bluuebunny/crossref_metadata_2025 huggingface dataset:
Treatment with direct oral anticoagulants or warfarin and the risk for incident diabetes among patients with atrial fibrillation: a population‐based cohort study
Authors: Cheung Ching-Lung , Sing Chor-Wing , Lau Wallis C. Y. , Li Gloria H. Y. , Lip Gregory Y. H. , Tan Kathryn C. B. , Cheung Bernard M. Y. , Chan Esther W. Y. , Wong Ian C. K.
Published: March 2021

Background

Diabetes mellitus is a common comorbidity of atrial fibrillation (AF), which can complicate the management of AF. The pharmacology of oral anticoagulants (OACs) have been implicated in pathogenesis of diabetes, but the relationship between different OACs and risk of diabetes remains unexamined. This study aimed to evaluate the risk of diabetes with use of different OACs in AF patients.

Methods

Population-based retrospective cohort study using an electronic healthcare database managed by the Hong Kong Hospital Authority. Patients newly diagnosed with AF from 2014 through 2018 and prescribed OACs were included and followed till December 31, 2019. Inverse probability of treatment weighting based on the propensity score (PS) is used to address potential bias due to nonrandomized allocation of treatment. The risks ofdiabetes were compared between different new OAC users using propensity score-weighted cumulative incidence differences (CID).

Results

There were 13,688 new users of OACs (warfarin: n = 3454; apixaban: n = 3335; dabigatran: n = 4210; rivaroxaban: n = 2689). The mean age was 75.0 (SD, 11.2), and 6,550 (47.9%) were women. After a median follow-up of 0.93 years (interquartile range, 0.21–1.92 years), 698 incident diabetes cases were observed. In Cox-regression analysis, dabigatran use was significantly associated with reduced risk of diabetes when compared with warfarin use [HR 0.69 (95% CI 0.56–0.86; P < 0.001)], with statistically insignificant associations observed for use of apixaban and rivaroxaban. The corresponding adjusted CIDs at 2 years after treatment with apixaban, dabigatran, and rivaroxaban users when compared with warfarin were − 2.06% (95% CI − 4.08 to 0.16%); − 3.06% (95% CI − 4.79 to − 1.15%); and − 1.8% (− 3.62 to 0.23%). In head-to-head comparisons between women DOAC users, dabigatran was also associated with a lower risk of diabetes when compared with apixaban and rivaroxaban.

Conclusions

Among adults with AF receiving OACs, the use of dabigatran had the lowest risk of diabetes when compared with warfarin use.
Correction to: Human drug efflux transporter ABCC5 confers acquired resistance to pemetrexed in breast cancer
Authors: Chen Jihui , Wang Zhipeng , Gao Shouhong , Wu Kejin , Bai Fang , Zhang Qiqiang , Wang Hongyu , Ye Qin , Xu Fengjing , Sun Hong , Lu Yunshu , Liu Yan
Published: March 2021

An amendment to this paper has been published and can be accessed via the original article.
Machine learning associated with respiratory oscillometry: a computer-aided diagnosis system for the detection of respiratory abnormalities in systemic sclerosis
Authors: Andrade Domingos S. M. , Ribeiro Luigi Maciel , Lopes Agnaldo J. , Amaral Jorge L. M. , Melo Pedro L.
Published: March 2021

Introduction

The use of machine learning (ML) methods would improve the diagnosis of respiratory changes in systemic sclerosis (SSc). This paper evaluates the performance of several ML algorithms associated with the respiratory oscillometry analysis to aid in the diagnostic of respiratory changes in SSc. We also find out the best configuration for this task.

Methods

Oscillometric and spirometric exams were performed in 82 individuals, including controls (n = 30) and patients with systemic sclerosis with normal (n = 22) and abnormal (n = 30) spirometry. Multiple instance classifiers and different supervised machine learning techniques were investigated, including k-Nearest Neighbors (KNN), Random Forests (RF), AdaBoost with decision trees (ADAB), and Extreme Gradient Boosting (XGB).

Results and discussion

The first experiment of this study showed that the best oscillometric parameter (BOP) was dynamic compliance, which provided moderate accuracy (AUC = 0.77) in the scenario control group versus patients with sclerosis and normal spirometry (CGvsPSNS). In the scenario control group versus patients with sclerosis and altered spirometry (CGvsPSAS), the BOP obtained high accuracy (AUC = 0.94). In the second experiment, the ML techniques were used. In CGvsPSNS, KNN achieved the best result (AUC = 0.90), significantly improving the accuracy in comparison with the BOP (p < 0.01), while in CGvsPSAS, RF obtained the best results (AUC = 0.97), also significantly improving the diagnostic accuracy (p < 0.05). In the third, fourth, fifth, and sixth experiments, different feature selection techniques allowed us to spot the best oscillometric parameters. They resulted in a small increase in diagnostic accuracy in CGvsPSNS (respectively, 0.87, 0.86, 0.82, and 0.84), while in the CGvsPSAS, the best classifier's performance remained the same (AUC = 0.97).

Conclusions

Oscillometric principles combined with machine learning algorithms provide a new method for diagnosing respiratory changes in patients with systemic sclerosis. The present study's findings provide evidence that this combination may help in the early diagnosis of respiratory changes in these patients.
Decomposition of high-frequency electrical conductivity into extracellular and intracellular compartments based on two-compartment model using low-to-high multi-b diffusion MRI
Authors: Lee Mun Bae , Kim Hyung Joong , Kwon Oh In
Published: March 2021

Background

As an object’s electrical passive property, the electrical conductivity is proportional to the mobility and concentration of charged carriers that reflect the brain micro-structures. The measured multi-b diffusion-weighted imaging (Mb-DWI) data by controlling the degree of applied diffusion weights can quantify the apparent mobility of water molecules within biological tissues. Without any external electrical stimulation, magnetic resonance electrical properties tomography (MREPT) techniques have successfully recovered the conductivity distribution at a Larmor-frequency.

Methods

This work provides a non-invasive method to decompose the high-frequency conductivity into the extracellular medium conductivity based on a two-compartment model using Mb-DWI. To separate the intra- and extracellular micro-structures from the recovered high-frequency conductivity, we include higher b-values DWI and apply the random decision forests to stably determine the micro-structural diffusion parameters.

Results

To demonstrate the proposed method, we conducted phantom and human experiments by comparing the results of reconstructed conductivity of extracellular medium and the conductivity in the intra-neurite and intra-cell body. The phantom and human experiments verify that the proposed method can recover the extracellular electrical properties from the high-frequency conductivity using a routine protocol sequence of MRI scan.

Conclusion

We have proposed a method to decompose the electrical properties in the extracellular, intra-neurite, and soma compartments from the high-frequency conductivity map, reconstructed by solving the electro-magnetic equation with measured B1 phase signals.
The Arab region’s contribution to global COVID-19 research: Bibliometric and visualization analysis
Authors: Zyoud Sa’ed H.
Published: March 2021

Background

At the global level and in the Arab world, particularly in low-income countries, COVID-19 remains a major public health issue. As demonstrated by an incredible number of COVID-19-related publications, the research science community responded rapidly. Therefore, this study was intended to assess the growing contribution of the Arab world to global research on COVID-19.

Methods

For the period between December 2019 and March 2021, the search for publications was conducted via the Scopus database using terms linked to COVID-19. VOSviewer 1.6.16 software was applied to generate a network map to assess hot topics in this area and determine the collaboration patterns between different countries. Furthermore, the research output of Arab countries was adjusted in relation to population size and gross domestic product (GDP).

Results

A total of 143,975 publications reflecting the global overall COVID-19 research output were retrieved. By restricting analysis to the publications published by the Arab countries, the research production was 6131 documents, representing 4.26% of the global research output regarding COVID-19. Of all these publications, 3990 (65.08%) were original journal articles, 980 (15.98%) were review articles, 514 (8.38%) were letters and 647 (10.55%) were others, such as editorials or notes. The highest number of COVID-19 publications was published by Saudi Arabia (n = 2186, 35.65%), followed by Egypt (n = 1281, 20.78%) and the United Arab Emirates (UAE), (n = 719, 11.73%). After standardization by population size and GDP, Saudi Arabia, UAE and Lebanon had the highest publication productivity. The collaborations were mostly with researchers from the United States (n = 968), followed by the United Kingdom (n = 661). The main research lines identified in COVID-19 from the Arab world are related to: public health and epidemiology; immunological and pharmaceutical research; signs, symptoms and clinical diagnosis; and virus detection.

Conclusions

A novel analysis of the latest Arab COVID-19-related studies is discussed in the current study and how these findings are connected to global production. Continuing and improving future collaboration between developing and developed countries will also help to facilitate the sharing of responsibilities for COVID-19 in research results and the implementation of policies for COVID-19.
Correction to: Leadership and capacity building in chiropractic research: report from the first CARL cohort
Authors: Hartvigsen Jan , Kawchuk Greg , Breen Alexander , De Carvalho Diana , Eklund Andreas , Fernandez Matthew , Funabashi Martha , Holmes Michelle M. , Johansson Melker S. , de Luca Katie , Moore Craig , Pagé Isabelle , Pohlman Katherine A. , Swain Michael S. , Wong Arnold Y. L. , Adams Jon
Published: March 2021

An amendment to this paper has been published and can be accessed via the original article.
Minimum 15-year results of metasul 28-mm metal-on-metal total hip arthroplasty in patients younger than 50 years of age
Authors: Jin Sheng-Yu , Jin Jing-Yao , Kang Joon-Kyoo , Yoon Taek-Rim , Park Kyung-Soon
Published: March 2021

Background

Some propitious mid- and long-term studies had been reported for MoM bearings; however, most studies have addressed specific patient groups rather than younger, active patients, who probably represent the most suitable population for investigations on wear and osteolysis. The purpose of this study to evaluate the long-term results of second-generation metal-on-metal cementless total hip arthroplasty (THA) in patients aged <50 years.

Methods

From December 1997 to January 2004, primary THA using a metal-on-metal bearing cementless implant was performed in 63 patients (72 hips) aged <50 years. The mean follow-up duration was 18.6 (range, 15.9–22.1) years, and the mean age at initial operation was 39 (range, 22–49) years. Clinical results, complications, survivorship, osteolysis, and aseptic loosening were evaluated.

Results

The mean Harris hip score and Western Ontario and McMaster Universities Arthritis Index scores were improved from 57.8 (range, 28–69) points and 73.4 (range, 63–94) points preoperatively to 91.7 (range, 80–100) points and 25.5 points (range, 17–38) points, respectively, at the last follow-up. Osteolysis lesions were found in 12 hips (acetabulum, 6 and femur, 6). The notching occurred on the femoral stem neck occurred in 12 hips. The mean serum cobalt and chromium concentrations were 2.3 (range, 0.2–10.6) μg/L and 1.7 (range, 0.4–8.1) μg/L, respectively, at a mean follow-up of 12.7 years in 32 patients (50.1%). The Kaplan-Meier survivorship curve analysis with revision for any reason as the endpoint revealed that 93.1% survived at 18.6 years’ follow-up.

Conclusions

Second-generation metal-on-metal cementless THA was found to produce satisfactory clinical and radiographic results with a low revision rate for osteolysis and aseptic loosening in patients aged less than 50 years.
Perforation of the atrial wall and aortic sinus after closure of an atrial septal defect with an Atriasept occluder: a case report
Authors: Zhang Zai-Qiang , Ding Jia-Wang
Published: March 2021

Background

While the perforation of the atrial wall and aortic sinus after closure of an atrial septal defect (ASD) is rare, it’s life-threatening, with rapid progress and high mortality. To the best of our knowledge, 21 similar cases have been reported since 1976.

Case presentation

We report a 16-year-old male whose atrial septal defect (ASD) was closed using a 12-mm Amplatzer septal occluder (ASO). Atrial wall and aortic sinus perforation occurred 3 months after transcatheter closure, and the patient was discharged after emergency operation. He was discharged on the 12th postoperative day in good overall condition.

Conclusions

With this case report, we want to illustrate that although percutaneous closure of ASD is regarded as a routine procedure, we should not forget the potentially lethal complications, especially cardiac erosion. Therefore, we should carefully evaluate the risk of erosion before surgery, and careful lifelong follow-up is needed.
The prevalence and distribution of human papillomavirus among 10,867 Chinese Han women
Authors:   CHIMUST team, Guo Chunlei , Du Hui , Belinson Jerome L. , Wang Chun , Huang Xia , Qu Xinfeng , Wu Ruifang
Published: March 2021

Objective

To assess the prevalence and distribution of HPV genotypes among Chinese Han women, and to explore the risk of high-grade cervical lesions associated with individual hr-HPV genotypes.

Methods

Genotyping and histopathology data from the Chinese Multi-Center Screening Trial (CHIMUST) and its pilot screening trial, from 6 regions across mainland China, were re-analyzed. The data from physician- and self-collected samples from 10,867 Chinese Han women (ages 30–69) were used to determine the prevalence and distribution of hr-HPV and to explore the risk association between hr-HPV genotypes and precancerous lesions.

Results

9.2% of the study population tested hr-HPV positive in physician-collected sample. The prevalence varied regionally from the lowest in Guangdong (6.3%) to the highest in Inner Mongolia (13.0%). The most prevalent genotypes found were HPV-52 (21.7%), HPV-16 (19.2%), HPV-58 (15.0%), HPV-39 (8.9%), and HPV-51 (8.2%). The overall odds ratios for CIN2+ and CIN3+ for the presence of HPV-16 was 58.6 (95% CI 39.2–87.5) and, 91.6 (95%CI 54.3–154.6), respectively and remained the highest odds ratio for CIN3+ in all 6 regions.

Conclusion

Geographical variation exists in the prevalence and distribution of hr-HPV in mainland China. HPV-16/52/58 were the most prevalent genotypes, and HPV-16 had the highest risk for high-grade cervical lesions.

Trial registration

CHIMUST, Registration number: ChiCTR-EOC-16008456. Registered 11 May 2016.
Compliance with American College of Chest Physicians (ACCP) recommendations for thromboembolic prophylaxis in the intensive care unit: a level I trauma center experience
Authors: Waxman Michael J. , Griffin Daniel , Sercy Erica , Bar-Or David
Published: March 2021

Background

Recommendations are for nearly universal venous thromboembolism (VTE) prophylaxis in critically ill hospitalized patients because of their well-recognized risks. In those intensive care units (ICUs) where patient care is more uniformly directed, it may be expected that VTE prophylaxis would more closely follow this standard over units that are less uniform, such as open-model ICUs.

Methods

This was a retrospective cohort study on all patients aged 18+ admitted to an open ICU between 6/1/2017 and 5/31/2018. Patients were excluded if they had instructions to receive comfort measures only or required therapeutic anticoagulant administration. Prophylaxis administration practices, including administration of mechanical and/or pharmacologic prophylaxis and delayed (≥48 h post-ICU admission) initiation of pharmacologic prophylaxis, were compared between patients admitted to the ICU by the trauma service versus other departments. Root causes for opting out of pharmacological prophylaxis were documented and compared between the two study groups.

Results

One-hundred two study participants were admitted by the trauma service, and 98 were from a non-trauma service. Mechanical (98% trauma vs. 99% non-trauma, P = 0.99) and pharmacologic (54% vs. 44%, P = 0.16) prophylaxis rates were similar between the two admission groups. The median time from ICU admission to pharmacologic prophylaxis initiation was 53 h for the trauma service and 10 h for the non–trauma services (P ≤ 0.01). In regression analyses, trauma-service admission (odds ratio (OR) = 2.88, 95% confidence interval (CI) 1.21–6.83) and increasing ICU length of stay (OR = 1.13, 95% CI 1.05–1.21) were independently associated with pharmacologic prophylaxis use. Trauma-service admission (OR = 8.30, 95% CI 2.18–31.56) and increasing hospital length of stay (OR = 1.15, 95% CI 1.03–1.28) were independently associated with delayed prophylaxis initiation.

Conclusions

Overall, the receipt of VTE prophylaxis of any type was close to 100%, due to the nearly universal use of mechanical compression devices among ICU patients in this study. However, when examining pharmacologic prophylaxis specifically, the rate was considerably lower than is currently recommended: 54% among the trauma services and 44% among non-trauma services.
ChrXq27.3 miRNA cluster functions in cancer development
Authors: Yoshida Kosuke , Yokoi Akira , Yamamoto Yusuke , Kajiyama Hiroaki
Published: March 2021

MicroRNAs (miRNAs) regulate the expression of their target genes post-transcriptionally; thus, they are deeply involved in fundamental biological processes. miRNA clusters contain two or more miRNA-encoding genes, and these miRNAs are usually coexpressed due to common expression mechanisms. Therefore, miRNA clusters are effective modulators of biological pathways by the members coordinately regulating their multiple target genes, and an miRNA cluster located on the X chromosome q27.3 region has received much attention in cancer research recently. In this review, we discuss the novel findings of the chrXq27.3 miRNA cluster in various types of cancer.

The chrXq27.3 miRNA cluster contains 30 mature miRNAs synthesized from 22 miRNA-encoding genes in an ~ 1.3-Mb region. The expressions of these miRNAs are usually negligible in many normal tissues, with the male reproductive system being an exception. In cancer tissues, each miRNA is dysregulated, compared with in adjacent normal tissues. The miRNA-encoding genes are not uniformly distributed in the region, and they are further divided into two groups (the miR-506-514 and miR-888-892 groups) according to their location on the genome. Most of the miRNAs in the former group are tumor-suppressive miRNAs that are further downregulated in various cancers compared with normal tissues. miR-506-3p in particular is the most well-known miRNA in this cluster, and it has various tumor-suppressive functions associated with the epithelial–mesenchymal transition, proliferation, and drug resistance. Moreover, other miRNAs, such as miR-508-3p and miR-509-3p, have similar tumor-suppressive effects. Hence, the expression of these miRNAs is clinically favorable as prognostic factors in various cancers. However, the functions of the latter group are less understood. In the latter group, miR-888-5p displays oncogenic functions, whereas miR-892b is tumor suppressive. Therefore, the functions of the miR-888–892 group are considered to be cell type- or tissue-specific.

In conclusion, the chrXq27.3 miRNA cluster is a critical regulator of cancer progression, and the miRNAs themselves, their regulatory mechanisms, and their target genes might be promising therapeutic targets.
CDKN2A germline alterations and the relevance of genotype-phenotype associations in cancer predisposition
Authors: Chan Sock Hoai , Chiang Jianbang , Ngeow Joanne
Published: March 2021

AlthoughCDKN2Ais well-known as a susceptibility gene for melanoma and pancreatic cancer, germline variants have also been anecdotally associated with a broader range of neoplasms including neural system tumors, head and neck squamous cell carcinomas, breast carcinomas, as well as sarcomas. TheCDKN2Agene encodes for two distinct tumor suppressor proteins, p16INK4Aand p14ARF, however, the independent association of germline alterations affecting these two proteins with cancer is under-appreciated. Here, we reviewedCDKN2Agermline alterations reported among individuals and families with cancer in the literature, specifically addressing the cancer phenotypes in relation to the molecular consequence on p16INK4Aand p14ARF. While melanoma is observed to associate with variants affecting both p16INK4Aand p14ARFtranscripts, it is noted that variants affecting p14ARFare more frequently observed with a heterogenous range of cancers. Finally, we reflected on the implications of this inferred genotype-phenotype association in clinical practice and proposed that clinical management ofCDKN2Agermline variant carriers should involve dedicated cancer genetics services, with multidisciplinary input from various healthcare professionals.
Hematologists’ barriers and enablers to screening and recruiting patients to a chimeric antigen receptor (CAR) T cell therapy trial: a theory-informed interview study
Authors:   on behalf of the GO CART team, Castillo Gisell , Lalu Manoj , Asad Sarah , Foster Madison , Kekre Natasha , Fergusson Dean , Hawrysh Terry , Atkins Harold , Thavorn Kednapa , Montroy Joshua , Schwartz Stuart , Holt Robert , Broady Raewyn , Presseau Justin
Published: March 2021

Background

Novel therapies often fail to reach the bedside due to low trial recruitment rates. Prior to conducting one of the first chimeric antigen receptor (CAR) T cell therapy trials in Canada, we used the Theoretical Domains Framework, a novel tool for identifying barriers and enablers to behavior change, to identify physician-related barriers and enablers to screening and recruiting patients for an early phase immunotherapy trial.

Methods

We conducted interviews with hematologists across Canada and used a directed content analysis to identify relevant domains reflecting the key factors that may affect screening and recruitment.

Results

In total, we interviewed 15 hematologists. Physicians expressed “cautious hope”; while expressing safety, feasibility, and screening criteria concerns, 14 out of 15 hematologists intended to screen for the trial (domains: knowledge, goals, beliefs about consequences, intentions). Physicians underscored the “challenging contexts,” identifying resources, workload, forgetting, and patient wait times to receive CAR T cells as key practical barriers to screening (domains: environmental context and resources, memory, attention and decision-making, behavioral regulation). They also highlighted “variability in roles and procedures” that may lead to missed trial candidates (domain: social and professional role). Left unaddressed, these barriers may undermine trial recruitment.

Conclusions

This study is among the first to use the Theoretical Domains Framework from the physician perspective to identify recruitment challenges to early phase trials and demonstrates the value of this approach for identifying barriers to screening and recruitment that may not otherwise have been elicited. This approach can optimize trial procedures and may serve to inform future promising early phase cancer therapy trials.

Trial registration

ClinicalTrials.gov Identifier: NCT03765177. Registered on December 5, 2018.
Determinants of adherence to dental treatment of socially vulnerable adolescents: a cohort study
Authors: Bulgareli Jaqueline Vilela , Cortellazzi Karine Laura , Guerra Luciane Miranda , Ambrosano Gláucia Maria Bovi , Kaieda Armando Koichiro , da Cunha Inara Pereira , Vazquez Fabiana de Lima , Pereira Antonio Carlos
Published: March 2021

Objective

Different studies with adolescents address the difficulty they have to adhere to oral dental treatments. Therefore, better understanding the processes involved in adherence to treatment in this population is necessary. The aim of this study was to investigate the factors that influence adherence to dental treatment in socially underprivileged adolescents in primary care.

Results

Non-adherence to treatment showed high rate in the studied sample (49.5%). Family income (p = 0.039) and number of individuals in the family (p = 0.003) were associated with non-adherence to dental treatment. It is concluded that the adolescents’ social vulnerability condition resulted in situations that are incompatible with adherence, which hinders dental treatment and health service planning.
Synchronous distance teaching of radiology clerkship promotes medical students’ learning and engagement
Authors: Alamer Ali , Alharbi Fawaz
Published: March 2021

Background

The COVID-19 pandemic has impacted education in myriad ways, primarily leading to an abrupt paradigm shift in teaching and learning practices towards distance learning. The study aims to assess the effectiveness of teaching radiology to undergraduate medical students using synchronous distance learning compared to traditional on-campus learning through exploring students’ perceived satisfaction and concerns. Students’ perceptions were correlated with their attendance, grades, and frequency of technical difficulties.

Methods

The study was designed as an observational study involving fourth-year medical students (2019/2020) from two institutions. The cohort students were exposed to traditional learning, distance learning, or both. Students completed an online self-administered questionnaire concerning their perceptions of distance learning. Students’ attendance, engagement, technical difficulties, and post-clerkship knowledge assessments were analyzed using descriptive and inferential statistics.

Results

A total of 145 participants completed the clerkship using the following strategies: traditional learning (n = 66), both traditional and distance learning (n = 67), and distance learning alone (n = 12). The most important result indicates that the abrupt transition to distance learning was well perceived. Most students preferred distance learning over traditional learning in the radiology clerkship (p = .05). During the synchronous sessions, student attendance was high, reaching to 100%. Technical difficulties were limited (1.9%), and they did not affect learning.

Conclusion

Synchronous distance teaching promotes learning, interaction, and enjoyment in undergraduate radiology education, and it can be as effective as traditional on-campus learning. The technical difficulties encountered, although they were limited, can be overcome by recording the synchronous sessions.
Natural killer cell therapy for hematologic malignancies: successes, challenges, and the future
Authors: Lamb Margaret G. , Rangarajan Hemalatha G. , Tullius Brian P. , Lee Dean A.
Published: March 2021

The adoptive transfer of natural killer (NK) cells is an emerging therapy in the field of immuno-oncology. In the last 3 decades, NK cells have been utilized to harness the anti-tumor immune response in a wide range of malignancies, most notably with early evidence of efficacy in hematologic malignancies. NK cells are dysfunctional in patients with hematologic malignancies, and their number and function are further impaired by chemotherapy, radiation, and immunosuppressants used in initial therapy and hematopoietic stem cell transplantation. Restoring this innate immune deficit may lead to improved therapeutic outcomes. NK cell adoptive transfer has proven to be a safe in these settings, even in the setting of HLA mismatch, and a deeper understanding of NK cell biology and optimized expansion techniques have improved scalability and therapeutic efficacy. Here, we review the use of NK cell therapy in hematologic malignancies and discuss strategies to further improve the efficacy of NK cells against these diseases.
Applications and therapeutic mechanisms of action of mesenchymal stem cells in radiation-induced lung injury
Authors: Niu Shiying , Zhang Yueying
Published: March 2021

Radiation-induced lung injury (RILI) is one of the most common complications associated with radiotherapy, characterized by early-stage radiation pneumonia and subsequent radiation pulmonary fibrosis. However, effective therapeutic strategies for RILI are currently lacking. Recently, an increasing number of studies reported that mesenchymal stem cells (MSCs) can enhance the regeneration of damaged tissue, modulate the inflammatory response, reduce the levels of fibrotic cytokines and reactive oxygen species, and inhibit epithelial-mesenchymal transformation. Interestingly, MSCs can also exert immunosuppressive effects, which highlights a new potential therapeutic activity of MSCs for managing RILI. Here, we reviewed the potential applications and therapeutic mechanisms of action of MSCs in RILI, which will represent a good compendium of information for researchers in this field.
Human AZFb deletions cause distinct testicular pathologies depending on their extensions in Yq11 and the Y haplogroup: new cases and review of literature
Authors: Vogt P. H. , Bender U. , Deibel B. , Kiesewetter F. , Zimmer J. , Strowitzki T.
Published: March 2021

Genomic AZFb deletions in Yq11 coined “classical” (i.e. length of Y DNA deletion: 6.23 Mb) are associated with meiotic arrest (MA) of patient spermatogenesis, i.e., absence of any postmeiotic germ cells. These AZFb deletions are caused by non-allelic homologous recombination (NAHR) events between identical sequence blocks located in the proximal arm of the P5 palindrome and within P1.2, a 92 kb long sequence block located in the P1 palindrome structure of AZFc in Yq11. This large genomic Y region includes deletion of 6 protein encoding Y genes, EIFA1Y, HSFY, PRY, RBMY1, RPS4Y, SMCY. Additionally, one copy of CDY2 and XKRY located in the proximal P5 palindrome and one copy of BPY1, two copies of DAZ located in the P2 palindrome, and one copy of CDY1 located proximal to P1.2 are included within this AZFb microdeletion. It overlaps thus distally along 2.3 Mb with the proximal part of the genomic AZFc deletion. However, AZFb deletions have been also reported with distinct break sites in the proximal and/or distal AZFb breakpoint intervals on the Y chromosome of infertile men. These so called “non-classical” AZFb deletions are associated with variable testicular pathologies, including meiotic arrest, cryptozoospermia, severe oligozoospermia, or oligoasthenoteratozoospermia (OAT syndrome), respectively. This raised the question whether there are any specific length(s) of the AZFb deletion interval along Yq11 required to cause meiotic arrest of the patient’s spermatogenesis, respectively, whether there is any single AZFb Y gene deletion also able to cause this “classical” AZFb testicular pathology? Review of the literature and more cases with “classical” and “non-classical” AZFb deletions analysed in our lab since the last 20 years suggests that the composition of the genomic Y sequence in AZFb is variable in men with distinct Y haplogroups especially in the distal AZFb region overlapping with the proximal AZFc deletion interval and that its extension can be “polymorphic” in the P3 palindrome. That means this AZFb subinterval can be rearranged or deleted also on the Y chromosome of fertile men. Any AZFb deletion observed in infertile men with azoospermia should therefore be confirmed as “de novo” mutation event, i.e., not present on the Y chromosome of the patient’s father or fertile brother before it is considered as causative agent for man’s infertility. Moreover, its molecular length in Yq11 should be comparable to that of the “classical” AZFb deletion, before meiotic arrest is prognosed as the patient’s testicular pathology.
Functional analysis of SARS-CoV-2 proteins in Drosophila identifies Orf6-induced pathogenic effects with Selinexor as an effective treatment
Authors: Zhu Jun-yi , Lee Jin-Gu , van de Leemput Joyce , Lee Hangnoh , Han Zhe
Published: March 2021

Background

SARS-CoV-2 causes COVID-19 with a widely diverse disease profile that affects many different tissues. The mechanisms underlying its pathogenicity in host organisms remain unclear. Animal models for studying the pathogenicity of SARS-CoV-2 proteins are lacking.

Methods

Using bioinformatic analysis, we found that 90% of the virus-host interactions involve human proteins conserved inDrosophila. Therefore, we generated a series of transgenic fly lines for individual SARS-CoV-2 genes, and used the Gal4-UAS system to express these viral genes inDrosophilato study their pathogenicity.

Results

We found that the ubiquitous expression of Orf6, Nsp6 or Orf7a inDrosophilaled to reduced viability and tissue defects, including reduced trachea branching as well as muscle deficits resulting in a “held-up” wing phenotype and poor climbing ability. Furthermore, muscles in these flies showed dramatically reduced mitochondria. Since Orf6 was found to interact with nucleopore proteins XPO1, we tested Selinexor, a drug that inhibits XPO1, and found that it could attenuate the Orf6-induced lethality and tissue-specific phenotypes observed in flies.

Conclusions

Our study establishedDrosophilaas a model for studying the function of SARS-CoV2 genes, identified Orf6 as a highly pathogenic protein in various tissues, and demonstrated the potential of Selinexor for inhibiting Orf6 toxicity using an in vivo animal model system.
Three-phase distribution transformer connections modeling based on matrix operation method by phase-coordinates
Authors: Zhang Zhigang , Mo Mingrui , Wu Caizhu
Published: March 2021

This paper proposes a matrix operation method for modeling the three-phase transformer by phase-coordinates. Based on decoupling theory, the 12 × 12 dimension primitive admittance matrix is obtained at first employing the coupling configuration of the windings. Under the condition of asymmetric magnetic circuits, according to the boundary conditions for transformer connections, the transformers in different connections enable to be modeling by the matrix operation method from the primitive admittance matrix. Another purpose of this paper is to explain the differences of the phase-coordinates and the positive sequence parameters in the impedances of the transformers. The numerical testing results in IEEE-4 system show that the proposed method is valid and efficient.
An improvement of convergence rate in the local limit theorem for integral-valued random variables
Authors: Siripraparat Tatpon , Neammanee Kritsana
Published: March 2021

Let $X_{1}, X_{2}, \ldots , X_{n}$

X
1

,

X
2

,
…
,

X
n

be independent integral-valued random variables, and let $S_{n}=\sum_{j=1}^{n}X_{j}$

S
n

=

∑

j
=
1

n

X
j

. One of the interesting probabilities is the probability at a particular point, i.e., the density of $S_{n}$

S
n

. The theorem that gives the estimation of this probability is called the local limit theorem. This theorem can be useful in finance, biology, etc. Petrov (Sums of Independent Random Variables, 1975) gave the rate $O (\frac{1}{n} )$
O
(

1
n

)
of the local limit theorem with finite third moment condition. Most of the bounds of convergence are usually defined with the symbol O. Giuliano Antonini and Weber (Bernoulli 23(4B):3268–3310, 2017) were the first who gave the explicit constant C of error bound $\frac{C}{\sqrt{n}}$

C

n

. In this paper, we improve the convergence rate and constants of error bounds in local limit theorem for $S_{n}$

S
n

. Our constants are less complicated than before, and thus easy to use.
Traveling wave solutions for a class of reaction-diffusion system
Authors: Wang Bingyi , Zhang Yang
Published: March 2021

In this paper we investigate the existence of traveling wave for a one-dimensional reaction diffusion system. We show that this system has a unique translation traveling wave solution.
Dynamics of a stochastic population model with predation effects in polluted environments
Authors: Song Guanghai
Published: March 2021

The present paper puts forward and probes a stochastic single-species model with predation effect in a polluted environment. We propose a threshold between extermination and weak persistence of the species and provide sufficient conditions for the stochastic persistence of the species. In addition, we evaluate the growth rates of the solution. Theoretical findings are expounded by some numerical simulations.
Factors that facilitate multigenerational exchanges in regional locations: a cross‐sectional study in Niigata City, Japan
Authors: Morita Kumiko , Kobayashi Minako , Aoki Rieko , Nagamine Hitomi , Yamamoto Harumi , Ohtake Fumi , Hoki Mika , Sumita Hiroko , Maruyama Kayo , Mitsuhashi Kayoko , Sasaki Akiko
Published: March 2021

Background

In the process of community building, it is important to create a place for multigenerational exchanges. To promote multigenerational exchanges in regional locations, it is essential to clarify whether such exchanges are related to government infrastructure, regional characteristics, and social capital, and how these exchanges contribute to community building.

Methods

A cross-sectional questionnaire study was conducted with representatives from 455 Chiiki no Cha-no-Ma (literal translation “community living room,” and hereafter “Cha-no-Ma”) in Niigata City, Japan. Responses were received from 405 representatives (response rate: 89.0 %), and 401 agreed to participate (4 declined). The survey details included basic information (e.g., date each location was established, frequency of meetings, number of caretakers and participants, qualifications of the representative), activities reflecting local culture, a social capital scale, the effects of the Cha-no-Ma implemented by the representative (12 items), challenges for management (16 items), and the implementation of multigenerational exchanges.

Results

Most of the age groups that participated in the Cha-no-Ma were elderly, and multigenerational exchanges took place in 125 locations (31.5 %). Items that had a significant connection to the implementation of multigenerational exchanges were “Frequency of meetings” (p < 0.001) and “Activities reflecting local culture” (p = 0.026). Binomial logistic regression analysis indicated that a high frequency of meetings was associated with the implementation of multigenerational exchanges (Odds ratio = 3.839).

There was a significantly higher ratio of implementation of multigenerational exchanges when the effects were a “connection with the region” (p = 0.006) and “conversations with different generations” (p = 0.004), and when the challenge was “no support from residents” (p = 0.002).

Conclusions

Cha-no-Ma participation is low among young people. The following ideas can be considered in order to increase multigenerational exchanges in regional locations. These exchanges may be promoted by increasing the frequency of meetings with qualified personnel and by adding activities that reflect local culture, such as festivals and making local foods. This community-based study clearly indicates that implementing multigenerational exchanges is an important activity for community building because it is related to connection within the community.
Patients with severe schistosomiasis mansoni in Ituri Province, Democratic Republic of the Congo
Authors: Nigo Maurice M. , Odermatt Peter , Nigo David Wully , Salieb-Beugelaar Georgette B. , Battegay Manuel , Hunziker Patrick R.
Published: March 2021

Background

Severe hepatosplenic complications arise in patients with chronic Schistosoma mansoni infection after heavy exposure to disease agents in endemic areas. These complications are rarely reported and, hence, underestimated.

Case presentation

We report on eight patients with severe morbidity associated with S. mansoni infection in Ituri Province, northeastern Democratic Republic of Congo (DRC). The patients were identified during a community-based survey in 2017; one patient was seen at the district hospital. After taking the patients’ history, a clinical examination and an abdominal ultrasonographical examination were performed. S. mansoni infection was diagnosed in fecal (Kato-Katz technique) and urine (point-of-case circulating cathodic antigen test) samples. These eight patients with severe intestinal and hepatosplenic complications were identified from four villages with high S. mansoni infection prevalence and related morbidity. The patients’ ages ranged from 19 to 57 years; four patients were women. Three patients reported hematemesis. Two patients were severely anemic. All patients reported non-specific abdominal symptoms, such as diarrhea (six patients), abdominal pain (seven patients), and blood in the stool (five patients), as well as weight loss (two patients). Abdominal ultrasonography revealed ascites in four patients. All patients had portal hypertension with hepatomegaly (seven patients) or splenomegaly (five patients). Of the six patients with a discernable liver parenchyma pattern, five displayed pattern F and three patient displayed pattern E. Liver parenchyma was not visible for two patients with severe ascites. An S. mansoni infection was confirmed in six patients, with infection intensity ranging from light to heavy. All S. mansoni positive patients were treated with praziquantel (40 mg/kg body weight) and referred to the district hospital for follow-up. One patient with severe ascites died two weeks after we saw her. Due to security and accessibility reasons, the villages could not be visited again and the patients were lost to follow-up.

Conclusions

Our observations of patients with severe schistosomiasis document the severe degree of endemicity of S. mansoni in the province and suggest an urgent need for adequate schistosomiasis control measures that target vulnerable population groups and address severe complications.
Quality of life after Supracricoid Partial Laryngectomy
Authors: Nakai Marianne Yumi , Menezes Marcelo Benedito , de Carvalho Julia Vilas Boas Gonçalves , Dias Lucas Porto Maurity , de Barros Silva Leandro Augusto , Tenório Lucas Ribeiro , Gonçalves Antonio José
Published: March 2021

Background

Supracricoid partial laryngectomy has good oncologic results in the treatment of advanced laryngeal cancer with the advantage of preserving larynx phonatory function when compared with total laryngectomy. However the rehabilitation could be a challenge, especially regarding swallowing function. Is supracricoid partial laryngectomy associated with better quality of life than total laryngectomy?

Methods

Survey study that included 33 patients (16 total laryngectomy and 17 supracricoid partial laryngectomy) with advanced larynx cancer surgically treated and fully rehabilitated. The quality of life were evaluated with EORTC QLQ C30 and H&N 35 instrument.

Results

Patients who underwent supracricoid partial laryngectomy obtained better scores in global health status-quality of life and general activities and had lower levels of sensory and speech-related symptoms.

Conclusion

SPL was associated with better quality of life when compared with TL.

Graphical Abstract
Insight into the current practice of ototoxicity monitoring during cisplatin therapy
Authors: Santucci N. M. , Garber B. , Ivory R. , Kuhn M. A. , Stephen M. , Aizenberg D.
Published: March 2021

Background

The aim of this study is to evaluate the current state of ototoxicity monitoring for patients receiving cisplatin chemotherapy in an academic medical center with particular attention to how closely monitoring adheres to national ototoxicity guidelines.

Methods

Case series including retrospective medical records review of patients (age > 18) treated with cisplatin at University of California Davis Medical Center between January 2014 and August 2017. Patient and ototoxicity related variables were analyzed. Patients that underwent a transfer of care during treatment and with less than 3 months of follow-up were excluded.

Results

Three hundred seventy-nine patients met study criteria, of which 104 (27.4%) had a prior history of hearing loss. Prior to treatment, 196 (51.7%) patients were counseled regarding the ototoxic nature of cisplatin and 92 (24.3%) patients had a pretreatment audiogram. During treatment, 91 (24%) patients had documented otologic complaints. Only 17 patients (4.5%) patients had an audiogram ordered during their cisplatin treatment period. 130 (34.3%) patients had otologic complaints following cisplatin treatment. Audiograms were ordered for 20 (7.8%), 13 (5.1%), and 16 (6.2%) patients at 1-month, 3-month, and 6-month follow-ups, respectively. No patients in the study cohort received baseline, treatment, and post-treatment audiograms as recommended by national ototoxicity monitoring protocols. Patients with Head and Neck Cancer (HNC) represented the largest subgroup that received cisplatin ( n = 122, 32.2%) and demonstrated higher rates of ototoxicity counseling ( n = 103, 84.4%) and pretreatment audiograms ( n = 70, 57.4%) compared to the non HNC group ( n = 36, 36.2%, P < 0.0001 and n = 22, 8.5%, P < 0.0001).

Conclusions

There is poor adherence to national ototoxicity monitoring guidelines at a large academic medical center. This is a missed opportunity for intervention and aural rehabilitation. Improved education and collaboration between otolaryngology, audiology, and medical oncology is needed to develop and promote an effective ototoxicity-monitoring program.

Graphical Abstract
The effect of using online language-support resources on L2 writing performance
Authors: Shin Dongkwang , Kwon Suh Keong , Lee Yongsang
Published: March 2021

Language learners today have access to a much wider choice of resources and language-support tools that aid their writing processes. While research on the convenience and flexibility of the writing resources that learners use in real-life writing activities have argued for a re-conceptualization of writing ability, little is known about the effect of using them in a writing assessment setting. Accordingly, the present study aimed to examine whether L2 learners’ use of writing resources in a writing test might have an effect on their writing performance. Furthermore, we also investigated how the effects of the writing resources might vary by test takers’ writing proficiency and scoring domains. Results showed that the group who had access to writing resources outperformed the group who were not given the online resource, but both groups’ scores were within the same scoring descriptor. The significant score improvement was more evident in the low- and intermediate-level learners. In a questionnaire, test takers reported that they normally use the writing resources in real-life writing situations and also found the online resource features helpful in improving their writing performance and agreed upon introducing such features in future writing tests.
Drone and sensor technology for sustainable weed management: a review
Authors: Esposito Marco , Crimaldi Mariano , Cirillo Valerio , Sarghini Fabrizio , Maggio Albino
Published: March 2021

Weeds are amongst the most impacting abiotic factors in agriculture, causing important yield loss worldwide. Integrated Weed Management coupled with the use of Unmanned Aerial Vehicles (drones), allows for Site-Specific Weed Management, which is a highly efficient methodology as well as beneficial to the environment. The identification of weed patches in a cultivated field can be achieved by combining image acquisition by drones and further processing by machine learning techniques. Specific algorithms can be trained to manage weeds removal by Autonomous Weeding Robot systems via herbicide spray or mechanical procedures. However, scientific and technical understanding of the specific goals and available technology is necessary to rapidly advance in this field. In this review, we provide an overview of precision weed control with a focus on the potential and practical use of the most advanced sensors available in the market. Much effort is needed to fully understand weed population dynamics and their competition with crops so as to implement this approach in real agricultural contexts.