bluuebunny/crossref_metadata_2025 huggingface dataset:
A Narrative Review on the Pathophysiology of Preeclampsia
Authors: Torres-Torres Johnatan , Espino-y-Sosa Salvador , Martinez-Portilla Raigam , Borboa-Olivares Hector , Estrada-Gutierrez Guadalupe , Acevedo-Gallegos Sandra , Ruiz-Ramirez Erika , Velasco-Espin Martha , Cerda-Flores Pablo , Ramirez-Gonzalez Andrea , Rojas-Zepeda Lourdes
Published: July 2024

Preeclampsia (PE) is a multifactorial pregnancy disorder characterized by hypertension and proteinuria, posing significant risks to both maternal and fetal health. Despite extensive research, its complex pathophysiology remains incompletely understood. This narrative review aims to elucidate the intricate mechanisms contributing to PE, focusing on abnormal placentation, maternal systemic response, oxidative stress, inflammation, and genetic and epigenetic factors. This review synthesizes findings from recent studies, clinical trials, and meta-analyses, highlighting key molecular and cellular pathways involved in PE. The review integrates data on oxidative stress biomarkers, angiogenic factors, immune interactions, and mitochondrial dysfunction. PE is initiated by poor placentation due to inadequate trophoblast invasion and improper spiral artery remodeling, leading to placental hypoxia. This triggers the release of anti-angiogenic factors such as soluble fms-like tyrosine kinase-1 (sFlt-1) and soluble endoglin (sEng), causing widespread endothelial dysfunction and systemic inflammation. Oxidative stress, mitochondrial abnormalities, and immune dysregulation further exacerbate the condition. Genetic and epigenetic modifications, including polymorphisms in the Fms-like tyrosine kinase 1 (FLT1) gene and altered microRNA (miRNA) expression, play critical roles. Emerging therapeutic strategies targeting oxidative stress, inflammation, angiogenesis, and specific molecular pathways like the heme oxygenase-1/carbon monoxide (HO-1/CO) and cystathionine gamma-lyase/hydrogen sulfide (CSE/H2S) pathways show promise in mitigating preeclampsia’s effects. PE is a complex disorder with multifactorial origins involving abnormal placentation, endothelial dysfunction, systemic inflammation, and oxidative stress. Despite advances in understanding its pathophysiology, effective prevention and treatment strategies remain limited. Continued research is essential to develop targeted therapies that can improve outcomes for both mothers and their babies.
Single-Molecule Mixture: A Concept in Polymer Science
Authors: Tang Yu
Published: July 2024

In theory, two extreme forms of substances exist: the pure form and the single-molecule mixture form. The latter contains a mixture of molecules with molecularly different structures. Inspired by the “chemical space” concept, in this paper, I report a study of the single-molecule mixture state that combines model construction and mathematical analysis, obtaining some interesting results. These results provide theoretical evidence that the single-molecule mixture state may indeed exist in realistic synthetic or natural polymer systems.
Innovative Analogs of Unpasteurized Kombucha Beverages: Comparative Analysis of Mint/Nettle Kombuchas, Considering Their Health-Promoting Effect, Polyphenolic Compounds and Chemical Composition
Authors: Pawluś Patrycja , Kolniak-Ostek Joanna
Published: July 2024

Increasing demand for functional beverages is attracting consumers’ attention and driving research to expand our knowledge of fermentation using symbiotic culture of bacteria and yeast (SCOBY) and demonstrate the health effects of consuming kombucha. The objective of this study was to develop innovative recipes for unpasteurized mint/nettle kombucha analogs, and to compare the products obtained under varying conditions in terms of chemical composition, bioactive polyphenols and health-promoting activity. Four variants of kombucha beverages (K1–K4), differing in the addition of sucrose and fermentation temperature, were formulated. The fermentation process provided data indicating the increase of antidiabetic, anti-inflammatory and anticholinergic properties, while a decrease in antioxidant capacity was observed. The content of polyphenolics was the highest on the seventh day of fermentation. A higher fermentation temperature and a larger amount of sucrose accelerated the fermentation process, which may be crucial for shortening the production time of kombucha drinks.
MEG3-Mediated Oral Squamous-Cell-Carcinoma-Derived Exosomal miR-421 Activates Angiogenesis by Targeting HS2ST1 in Vascular Endothelial Cells
Authors: Huang Chia-Yun , Chou Sung-Tau , Hsu Yuan-Ming , Chao Wan-Ju , Wu Guan-Hsun , Hsiao Jenn-Ren , Wang Horng-Dar , Shiah Shine-Gwo
Published: July 2024

Exosomal microRNAs (miRNAs) from cancer cells play a key role in mediating the oral squamous cell carcinoma (OSCC) microenvironment. The objective of this study was to investigate how the long non-coding RNA (lncRNA) MEG3 affects OSCC angiogenesis through exosomal miR-421. Global miRNA microarray analysis and quantitative real-time PCR (qRT-PCR) were performed to determine the level of miRNAs in OSCC cell-derived exosomes. Cell migration, invasion, tube formation, immunohistochemistry, and hemoglobin concentrations were used to study the effects of exosomal miR-421 in angiogenesis. Western blotting was used to determine the expression level of HS2ST1 and VEGFR2-related downstream proteins. MiRNA array and qRT-PCR identified the upregulation of miR-421 in OSCC cell-derived exosomes. Furthermore, exosomal miR-421 can be taken up by human umbilical vein endothelial cells (HUVECs) and then target HS2ST1 through VEGF-mediated ERK and AKT phosphorylation, thereby promoting HUVEC migration, invasion, and tube formation. Additionally, forced expression of the lncRNA MEG3 in OSCC cells reduced exosomal miR-421 levels and then increased HS2ST1 expression, thereby reducing the VEGF/VEGFR2 pathway in HUVECs. Our results demonstrate a novel mechanism by which lncRNA MEG3 can act as a tumor suppressor and regulate endothelial angiogenesis through the exosomal miR-421/HS2ST1 axis, which provides a potential therapeutic strategy for OSCC angiogenesis.
Influence of BMI, Cigarette Smoking and Cryopreservation on Tyrosine Phosphorylation during Sperm Capacitation
Authors: Ortiz-Vallecillo Ana , Santamaría-López Esther , García-Ruiz Diego , Martín-Lozano David , Candenas Luz , Pinto Francisco M. , Fernández-Sánchez Manuel , González-Ravina Cristina
Published: July 2024

Capacitation involves tyrosine phosphorylation (TP) as a key marker. Lifestyle-related factors, such as obesity and smoking, are recognized for their adverse effects on semen quality and male fertility, yet the underlying mechanisms, including their potential impact on TP, remain unclear. Moreover, the effect of sperm cryopreservation on TP at the human sperm population level is unexplored. Flow cytometry analysis of global TP was performed on pre-capacitated, post-capacitated and 1- and 3-hours’ incubated fresh and frozen–thawed samples from sperm donors (n = 40). Neither being overweight nor smoking (or both) significantly affected the percentage of sperm showing TP. However, elevated BMI and smoking intensity correlated with heightened basal TP levels (r = 0.226, p = 0.003) and heightened increase in TP after 3 h of incubation (r = 0.185, p = 0.017), respectively. Cryopreservation resulted in increased global TP levels after capacitation but not immediately after thawing. Nonetheless, most donors’ thawed samples showed increased TP levels before and after capacitation as well as after incubation. Additionally, phosphorylation patterns in fresh and frozen–thawed samples were similar, indicating consistent sample response to capacitation stimuli despite differences in TP levels. Overall, this study sheds light on the potential impacts of lifestyle factors and cryopreservation on the dynamics of global TP levels during capacitation.
Structure Engineering of Ni/SiO2 Vegetable Oil Hydrogenation Catalyst via CeO2
Authors: Gabrovska Margarita , Nikolova Dimitrinka , Radonjić Vojkan , Karashanova Daniela , Baeva Aleksandra , Parvanova-Mancheva Tsvetomila , Tzvetkov Peter , Petrova Evangeliya , Zarkova Gabriella , Krstić Jugoslav
Published: July 2024

Inspired by our finding that metallic Ni particles could be uniformly distributed on a reduced CeO2 surface and stabilized on Ce3+ sites, we suppose a possible improvement in the activity and selectivity of the MgNi/SiO2 vegetable oil hydrogenation catalyst by increasing the surface metal Ni availability via modification by ceria. The proposed approach involved the addition of a CeO2 modifier to the SiO2 carrier and as a catalyst component. Evaluation of the structure, reducibility, and surface and electronic states of the CeO2-doped MgNi/SiO2 catalyst was performed by means of the Powder X-ray diffraction (PXRD), Scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS), and X-ray photoelectron spectroscopy (XPS) combined with High-resolution transmission electron microscopy (HRTEM), Temperature-programmed reduction with hydrogen (H2-TPR), and H2-chemisortion techniques. So far, no studies related to this approach of designing Ni/SiO2 catalysts for the partial hydrogenation of vegetable oil have been reported. The added ceria impact was elucidated by comparing fatty acid compositions obtained by the catalysts at an iodine value of 80. In summary, tuning the hydrogenation performance of Ni-based catalysts can be achieved by structural reconstruction using 1 wt.% CeO2. The introduction mode changed the selectivity towards C18:1-cis and C18:0 fatty acids by applying ceria as a carrier modifier, while hydrogenation activity was improved upon ceria operation as the catalyst dopant.
Transcriptomic and Epigenomic Responses to Cortisol-Mediated Stress in Rainbow Trout (Oncorhynchus mykiss) Skeletal Muscle
Authors: Aravena-Canales Daniela , Valenzuela-Muñoz Valentina , Gallardo-Escarate Cristian , Molina Alfredo , Valdés Juan Antonio
Published: July 2024

The production and release of cortisol during stress responses are key regulators of growth in teleosts. Understanding the molecular responses to cortisol is crucial for the sustainable farming of rainbow trout (Oncorhynchus mykiss) and other salmonid species. While several studies have explored the genomic and non-genomic impacts of cortisol on fish growth and skeletal muscle development, the long-term effects driven by epigenetic mechanisms, such as cortisol-induced DNA methylation, remain unexplored. In this study, we analyzed the transcriptome and genome-wide DNA methylation in the skeletal muscle of rainbow trout seven days after cortisol administration. We identified 550 differentially expressed genes (DEGs) by RNA-seq and 9059 differentially methylated genes (DMGs) via whole-genome bisulfite sequencing (WGBS) analysis. KEGG enrichment analysis showed that cortisol modulates the differential expression of genes associated with nucleotide metabolism, ECM-receptor interaction, and the regulation of actin cytoskeleton pathways. Similarly, cortisol induced the differential methylation of genes associated with focal adhesion, adrenergic signaling in cardiomyocytes, and Wnt signaling. Through integrative analyses, we determined that 126 genes showed a negative correlation between up-regulated expression and down-regulated methylation. KEGG enrichment analysis of these genes indicated participation in ECM-receptor interaction, regulation of actin cytoskeleton, and focal adhesion. Using RT-qPCR, we confirmed the differential expression of lamb3, itga6, limk2, itgb4, capn2, and thbs1. This study revealed for the first time the molecular responses of skeletal muscle to cortisol at the transcriptomic and whole-genome DNA methylation levels in rainbow trout.
Mutations in Genes Encoding Subunits of the RNA Exosome as a Potential Novel Cause of Thrombotic Microangiopathy
Authors: Wijnsma Kioa L. , Schijvens Anne M. , Bouwmeester Romy N. , Aarts Lonneke A. M. , van den Heuvel Lambertus (Bert) P. , Haaxma Charlotte A. , van de Kar Nicole C. A. J.
Published: July 2024

Thrombotic microangiopathy (TMA) in association with RNA exosome encoding mutations has only recently been recognized. Here, we present an infant (female) with an EXOSC5 mutation (c.230_232del p.Glu77del) associated with the clinical phenotype known as CABAC syndrome (cerebellar ataxia, brain abnormalities, and cardiac conduction defects), including pontocerebellar hypoplasia, who developed renal TMA. At the age of four months, she presented with signs of septic illness, after which she developed TMA. A stool culture showed rotavirus as a potential trigger. The patient received eculizumab once, alongside supportive treatment, while awaiting diagnostic analysis of TMA, including genetic complement analysis, all of which were negative. Eculizumab was withdrawn and the patient’s TMA recovered quickly. A review of the literature identified an additional four patients (age < 1 year) who developed TMA after a viral trigger in the presence of mutations in EXOSC3. The recurrence of TMA in one of these patients with an EXOSC3 mutation while on eculizumab treatment underscores the apparent lack of responsiveness to C5 inhibition. In conclusion, mutations in genes influencing the RNA exosome, like EXOSC3 and EXOSC5, characterized by neurodevelopment and neurodegenerative disorders could potentially lead to TMA in the absence of complement dysregulation. Hence, these patients were likely non-responsive to eculizumab.
The Role of Lactobacillus plantarum in Reducing Obesity and Inflammation: A Meta-Analysis
Authors: Li Chen-Pi , Chen Chin-Chang , Hsiao Yao , Kao Chieh-Hsin , Chen Chin-Chu , Yang Hao-Jan , Tsai Ru-Yin
Published: July 2024

Recent research has underscored the efficacy of Lactobacillus plantarum (L. plantarum) in managing obesity among healthy adults. This meta-analysis reviewed randomized controlled trials (RCTs) from major databases up to May 2024, focusing on the effects of L. plantarum on body weight, body mass index (BMI), and metabolic parameters. This study has been registered in PROSPERO (number: CRD 42024531611). The analysis of nine studies revealed significant weight reduction and BMI decreases with L. plantarum supplementation compared to a placebo. Notably, using more than two strains together enhanced these effects. Improvements were also observed in abdominal fat and inflammatory markers such as interleukin-6 (IL-6) and high-sensitivity C-reactive protein (hs-CRP). This meta-analysis synthesizes evidence from nine RCTs to test the hypothesis that L. plantarum supplementation effectively reduces body weight and BMI in healthy adults compared to a placebo. However, variations in study designs, probiotic strains, and intervention durations call for more robust trials to confirm these benefits.
Entamoeba histolytica: EhADH, an Alix Protein, Participates in Several Virulence Events through Its Different Domains
Authors: Zanatta Dxinegueela , Betanzos Abigail , Azuara-Liceaga Elisa , Montaño Sarita , Orozco Esther
Published: July 2024

Entamoeba histolytica is the protozoan causative of human amoebiasis. The EhADH adhesin (687 aa) is a protein involved in tissue invasion, phagocytosis and host-cell lysis. EhADH adheres to the prey and follows its arrival to the multivesicular bodies. It is an accessory protein of the endosomal sorting complexes required for transport (ESCRT) machinery. Here, to study the role of different parts of EhADH during virulence events, we produced trophozoites overexpressing the three domains of EhADH, Bro1 (1–400 aa), Linker (246–446 aa) and Adh (444–687 aa) to evaluate their role in virulence. The TrophozBro11–400 slightly increased adherence and phagocytosis, but these trophozoites showed a higher ability to destroy cell monolayers, augment the permeability of cultured epithelial cells and mouse colon, and produce more damage to hamster livers. The TrophozLinker226–446 also increased the virulence properties, but with lower effect than the TrophozBro11–400. In addition, this fragment participates in cholesterol transport and GTPase binding. Interestingly, the TrophozAdh444–687 produced the highest effect on adherence and phagocytosis, but it poorly influenced the monolayers destruction; nevertheless, they augmented the colon and liver damage. To identify the protein partners of each domain, we used recombinant peptides. Pull-down assays and mass spectrometry showed that Bro1 domain interplays with EhADH, Gal/GalNAc lectin, EhCPs, ESCRT machinery components and cytoskeleton proteins. While EhADH, ubiquitin, EhRabB, EhNPC1 and EhHSP70 were associated to the Linker domain, and EhADH, EhHSP70, EhPrx and metabolic enzymes interacted to the Adh domain. The diverse protein association confirms that EhADH is a versatile molecule with multiple functions probably given by its capacity to form distinct molecular complexes.
Adjuvant Properties of Caffeic Acid in Cancer Treatment
Authors: Cortez Nicole , Villegas Cecilia , Burgos Viviana , Cabrera-Pardo Jaime R. , Ortiz Leandro , González-Chavarría Iván , Nchiozem-Ngnitedem Vaderament-A. , Paz Cristian
Published: July 2024

Caffeic acid (CA) is a polyphenol belonging to the phenylpropanoid family, commonly found in plants and vegetables. It was first identified by Hlasiwetz in 1867 as a breakdown product of caffetannic acid. CA is biosynthesized from the amino acids tyrosine or phenylalanine through specific enzyme-catalyzed reactions. Extensive research since its discovery has revealed various health benefits associated with CA, including its antioxidant, anti-inflammatory, and anticancer properties. These effects are attributed to its ability to modulate several pathways, such as inhibiting NFkB, STAT3, and ERK1/2, thereby reducing inflammatory responses, and activating the Nrf2/ARE pathway to enhance antioxidant cell defenses. The consumption of CA has been linked to a reduced risk of certain cancers, mitigation of chemotherapy and radiotherapy-induced toxicity, and reversal of resistance to first-line chemotherapeutic agents. This suggests that CA could serve as a useful adjunct in cancer treatment. Studies have shown CA to be generally safe, with few adverse effects (such as back pain and headaches) reported. This review collates the latest information from Google Scholar, PubMed, the Phenol-Explorer database, and ClinicalTrials.gov, incorporating a total of 154 articles, to underscore the potential of CA in cancer prevention and overcoming chemoresistance.
Comparative Analysis of Mitochondria Surrounding the Intercalated Discs in Heart Diseases—An Ultrastructural Pilot Study
Authors: Schönmehl Rebecca , Mendelsohn Daniel H. , Winter Lina , Pabel Steffen , Niedermair Tanja , Evert Katja , Cheung Wing-Hoi , Wong Ronald Man Yeung , Schmitt Volker H. , Keller Karsten , Barsch Friedrich , Dietl Alexander , Gummert Jan F. , Schramm René , Sossalla Samuel , Brochhausen Christoph
Published: July 2024

Background: Mitochondria play a crucial role in adapting to fluctuating energy demands, particularly in various heart diseases. This study investigates mitochondrial morphology near intercalated discs in left ventricular (LV) heart tissues, comparing samples from patients with sinus rhythm (SR), atrial fibrillation (AF), dilated cardiomyopathy (DCM), and ischemic cardiomyopathy (ICM). Methods: Transmission electron microscopy was used to analyze mitochondria within 0–3.5 μm and 3.5–7 μm of intercalated discs in 9 SR, 10 AF, 9 DCM, and 8 ICM patient samples. Parameters included mean size in µm2 and elongation, count, percental mitochondrial area in the measuring frame, and a conglomeration score. Results: AF patients exhibited higher counts of small mitochondria in the LV myocardium, resembling SR. DCM and ICM groups had fewer, larger, and often hydropic mitochondria. Accumulation rates and percental mitochondrial area were similar across groups. Significant positive correlations existed between other defects/size and hydropic mitochondria and between count/area and conglomeration score, while negative correlations between count and size/other defects and between hydropic mitochondria and count could be seen as well. Conclusion: Mitochondrial parameters in the LV myocardium of AF patients were similar to those of SR patients, while DCM and ICM displayed distinct changes, including a decrease in number, an increase in size, and compromised mitochondrial morphology. Further research is needed to fully elucidate the pathophysiological role of mitochondrial morphology in different heart diseases, providing deeper insights into potential therapeutic targets and interventions.
High-Intensity Focused Ultrasound Increases Facial Adipogenesis in a Swine Model via Modulation of Adipose-Derived Stem Cell Cilia
Authors: Byun Kyung-A , Kim Hyoung Moon , Oh Seyeon , Batsukh Sosorburam , Lee Sangsu , Oh Myungjune , Lee Jeongwoo , Lee Ran , Kim Jae Woo , Oh Seung Min , Kim Jisun , Kim Geebum , Park Hyun Jun , Hong Hanbit , Lee Jehyuk , An Sang-Hyun , Oh Sung Suk , Jung Yeon-Seop , Son Kuk Hui , Byun Kyunghee
Published: July 2024

Decreased medial cheek fat volume during aging leads to loss of a youthful facial shape. Increasing facial volume by methods such as adipose-derived stem cell (ASC) injection can produce facial rejuvenation. High-intensity focused ultrasound (HIFU) can increase adipogenesis in subcutaneous fat by modulating cilia on ASCs, which is accompanied by increased HSP70 and decreased NF-κB expression. Thus, we evaluated the effect of HIFU on increasing facial adipogenesis in swine (n = 2) via modulation of ASC cilia. Expression of CD166, an ASC marker, differed by subcutaneous adipose tissue location. CD166 expression in the zygomatic arch (ZA) was significantly higher than that in the subcutaneous adipose tissue in the mandible or lateral temporal areas. HIFU was applied only on the right side of the face, which was compared with the left side, where HIFU was not applied, as a control. HIFU produced a significant increase in HSP70 expression, decreased expression of NF-κB and a cilia disassembly factor (AURKA), and increased expression of a cilia increasing factor (ARL13B) and PPARG and CEBPA, which are the main regulators of adipogenesis. All of these changes were most prominent at the ZA. Facial adipose tissue thickness was also increased by HIFU. Adipose tissue volume, evaluated by magnetic resonance imaging, was increased by HIFU, most prominently in the ZA. In conclusion, HIFU increased ASC marker expression, accompanied by increased HSP70 and decreased NF-κB expression. Additionally, changes in cilia disassembly and length and expression of adipogenesis were observed. These results suggest that HIFU could be used to increase facial volume by modulating adipogenesis.
Potential for Drug Repositioning of Midazolam as an Inhibitor of Inflammatory Bone Resorption
Authors: Harigaya Hiroko , Chiba-Ohkuma Risako , Karakida Takeo , Yamamoto Ryuji , Fujii-Abe Keiko , Kawahara Hiroshi , Yamakoshi Yasuo
Published: July 2024

Drug repositioning is a method for exploring new effects of existing drugs, the safety and pharmacokinetics of which have been confirmed in humans. Here, we demonstrate the potential drug repositioning of midazolam (MDZ), which is used for intravenous sedation, as an inhibitor of inflammatory bone resorption. We cultured a mouse macrophage-like cell line with or without MDZ and evaluated its effects on the induction of differentiation of these cells into osteoclasts. For in vivo investigations, we administered lipopolysaccharide (LPS) together with MDZ (LPS+MDZ) to the parietal region of mice and evaluated the results based on the percentage of bone resorption and calvaria volume. Furthermore, we examined the effects of MDZ on the production of reactive oxygen species (ROS) in cells and on its signaling pathway. MDZ inhibited osteoclast differentiation and bone resorption activity. In animal studies, the LPS+MDZ group showed a decreasing trend associated with the rate of bone resorption. In addition, the bone matrix volume in the LPS+MDZ group was slightly higher than in the LPS only group. MDZ inhibited osteoclast differentiation by decreasing ROS production and thereby negatively regulating the p38 mitogen-activated protein kinase pathway. Thus, we propose that MDZ could potentially be used for treating inflammatory bone resorption, for example, in periodontal disease.
Understanding Autoimmunity: Mechanisms, Predisposing Factors, and Cytokine Therapies
Authors: Yasmeen Farzana , Pirzada Rameez Hassan , Ahmad Bilal , Choi Bogeum , Choi Sangdun
Published: July 2024

Autoimmunity refers to an organism’s immune response against its own healthy cells, tissues, or components, potentially leading to irreversible damage to vital organs. Central and peripheral tolerance mechanisms play crucial roles in preventing autoimmunity by eliminating self-reactive T and B cells. The disruption of immunological tolerance, characterized by the failure of these mechanisms, results in the aberrant activation of autoreactive lymphocytes that target self-tissues, culminating in the pathogenesis of autoimmune disorders. Genetic predispositions, environmental exposures, and immunoregulatory disturbances synergistically contribute to the susceptibility and initiation of autoimmune pathologies. Within the realm of immune therapies for autoimmune diseases, cytokine therapies have emerged as a specialized strategy, targeting cytokine-mediated regulatory pathways to rectify immunological imbalances. Proinflammatory cytokines are key players in inducing and propagating autoimmune inflammation, highlighting the potential of cytokine therapies in managing autoimmune conditions. This review discusses the etiology of autoimmune diseases, current therapeutic approaches, and prospects for future drug design.
Molecular Cloning and Functional Identification of a Pericarp- and Testa-Abundant Gene’s (AhN8DT-2) Promoter from Arachis hypogaea
Authors: Sharif Yasir , Zhuang Yuhui , Xie Wenpin , Zhang Chong , Chen Kun , Deng Ye , Chen Yuting , Fu Huiwen , Wang Lihui , Chen Xiangyu , Zhuang Weijian , Chen Hua
Published: July 2024

Cultivated peanut (Arachis hypogaea L.) is a key oil- and protein-providing legume crop of the world. It is full of nutrients, and its nutrient profile is comparable to that of other nuts. Peanut is a unique plant as it showcases a pegging phenomenon, producing flowers above ground, and after fertilization, the developing peg enters the soil and produces seeds underground. This geocarpic nature of peanut exposes its seeds to soil pathogens. Peanut seeds are protected by an inedible pericarp and testa. The pericarp- and testa-specific promoters can be effectively used to improve the seed defense. We identified a pericarp- and testa-abundant expression gene (AhN8DT-2) from available transcriptome expression data, whose tissue-specific expression was further confirmed by the qRT-PCR. The 1827bp promoter sequence was used to construct the expression vector using the pMDC164 vector for further analysis. Quantitative expression of the GUS gene in transgenic Arabidopsis plants showed its high expression in the pericarp. GUS staining showed a deep blue color in the pericarp and testa. Cryostat sectioning of stained Arabidopsis seeds showed that expression is only limited to seed coat (testa), and staining was not present in cotyledons and embryos. GUS staining was not detected in any other tissues, including seedlings, leaves, stems, and roots, except for some staining in flowers. Under different phytohormones, this promoter did not show an increase in expression level. These results indicated that the AhN8DT-2 promoter drives GUS gene expression in a pericarp- and testa-specific manner. The identified promoter can be utilized to drive disease resistance genes, specifically in the pericarp and testa, enhancing peanut seed defense against soil-borne pathogens. This approach has broader implications for improving the resilience of peanut crops and other legumes, contributing to sustainable agricultural practices and food security.
Apt-Conjugated PDMS-ZnO/Ag-Based Multifunctional Integrated Superhydrophobic Biosensor with High SERS Activity and Photocatalytic Sterilization Performance
Authors: Qian Sihan , Zhao Wenshi , Guo Rui , Wang Xiaohan , Dai Huasong , Lang Jihui , Kadasala Naveen Reddy , Jiang Yuhong , Liu Yang
Published: July 2024

Sensitive detection and efficient inactivation of pathogenic bacteria are crucial for halting the spread and reproduction of foodborne pathogenic bacteria. Herein, a novel Apt-modified PDMS-ZnO/Ag multifunctional biosensor has been developed for high-sensitivity surface-enhanced Raman scattering (SERS) detection along with photocatalytic sterilization towards Salmonella typhimurium (S. typhimurium). The distribution of the electric field in PDMS-ZnO/Ag with different Ag sputtering times was analyzed using a finite-difference time-domain (FDTD) algorithm. Due to the combined effect of electromagnetic enhancement and chemical enhancement, PDMS-ZnO/Ag exhibited outstanding SERS sensitivity. The limit of detection (LOD) for 4-MBA on the optimal SERS substrate (PZA-40) could be as little as 10−9 M. After PZA-40 was modified with the aptamer, the LOD of the PZA-40-Apt biosensor for detecting S. typhimurium was only 10 cfu/mL. Additionally, the PZA-40-Apt biosensor could effectively inactivate S. typhimurium under visible light irradiation within 10 min, with a bacterial lethality rate (Lb) of up to 97%. In particular, the PZA-40-Apt biosensor could identify S. typhimurium in food samples in addition to having minimal cytotoxicity and powerful biocompatibility. This work provides a multifunctional nanoplatform with broad prospects for selective SERS detection and photocatalytic sterilization of pathogenic bacteria.
Epigenome-Wide Association Study of Depressive Symptoms in Black Women in the InterGEN Study
Authors: Taylor Brittany , Zhao Yihong , Perez Nicole B. , Potts-Thompson Stephanie , Crusto Cindy , Creber Ruth Masterson , Taylor Jacquelyn Y.
Published: July 2024

(1) The prevalence of depression is two times higher in women than men. Black women have an increased risk of depression due to stressors such as low socioeconomic status and perceived discrimination. Depression is likely influenced by both genetic and environmental factors. Psychosocial stressors can influence DNA methylation (DNAm), leading to changes in gene expression and ultimately, depression. The objective of this study was to examine associations between DNAm and depressive symptoms in Black women. (2) This study was a secondary analysis of data from the Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure (InterGEN) Study. Perceived discrimination was assessed using Krieger’s Experiences of Discrimination and Waelde’s Race-Related Events Scale, and participants were screened for depressive symptoms with the Beck Depression Inventory. Raw data from saliva samples were analyzed using the Illumina Infinium Epic (850 K) BeadChip and then preprocessed in RStudio. (3) Differential methylation analysis identified DNAm sites and regions associated with depressive symptoms. Six DNAm sites had a q-value less than 0.05. Additionally, of the 25 regions identified, 12 were associated with neurological diseases or disorders. (4) These findings suggest that there is a neurological component to depression, which should be considered during treatment.
Nanofibrous Scaffolds in Biomedicine
Authors: Omidian Hossein , Gill Erma J.
Published: July 2024

This review explores the design, fabrication, and biomedical applications of nanofibrous scaffolds, emphasizing their impact on tissue engineering and regenerative medicine. Advanced techniques like electrospinning and 3D printing have enabled precise control over scaffold architecture, crucial for mimicking native tissue structures. Integrating bioactive materials has significantly enhanced cellular interactions, mechanical properties, and the controlled release of therapeutic agents. Applications span bone, cardiovascular, soft tissue, neural regeneration, wound healing, and advanced drug delivery. Despite these advancements, challenges such as scalability, biocompatibility, and long-term stability remain barriers to clinical translation. Future research should focus on developing smart scaffolds and utilizing AI-enhanced manufacturing for more personalized and effective regenerative therapies.
Sheep Bone Ultrastructure Analyses Reveal Differences in Bone Maturation around Mg-Based and Ti Implants
Authors: Iskhakova Kamila , Wieland D. C. Florian , Marek Romy , Schwarze Uwe Y. , Davydok Anton , Cwieka Hanna , AlBaraghtheh Tamadur , Reimers Jan , Hindenlang Birte , Sefa Sandra , Lopes Marinho André , Willumeit-Römer Regine , Zeller-Plumhoff Berit
Published: July 2024

Magnesium alloys are some of the most convenient biodegradable materials for bone fracture treatment due to their tailorable degradation rate, biocompatibility, and mechanical properties resembling those of bone. Despite the fact that magnesium-based implants and ZX00 (Mg-0.45Zn-0.45Ca in wt.%), in particular, have been shown to have suitable degradation rates and good osseointegration, knowledge gaps remain in our understanding of the impact of their degradation properties on the bone’s ultrastructure. Bone is a hierarchically structured material, where not only the microstructure but also the ultrastructure are important as properties like the local mechanical response are determined by it. This study presents the first comparative analysis of bone ultrastructure parameters with high spatial resolution around ZX00 and Ti implants after 6, 12, and 24 weeks of healing. The mineralization was investigated, revealing a significant decrease in the lattice spacing of the (002) Bragg’s peak closer to the ZX00 implant in comparison to Ti, while no significant difference in the crystallite size was observed. The hydroxyapatite platelet thickness and osteon density demonstrated a decrease closer to the ZX00 implant interface. Correlative indentation and strain maps obtained by scanning X-ray diffraction measurements revealed a higher stiffness and faster mechanical adaptation of the bone surrounding Ti implants as compared to the ZX00 ones. Thus, the results suggest the incorporation of Mg2+ ions into the bone ultrastructure, as well as a lower degree of remodeling and stiffness of the bone in the presence of ZX00 implants than Ti.
The Influence of the Process Conditions on the Thermo-Mechanical Fatigue Damage of the Rolls in the Twin-Roll Casting Process of Aluminum Alloys
Authors: Shevchenko Ratibor , Zani Nicola , Mazzù Angelo
Published: July 2024

Twin-roll casting is a technology for the production of thin strips directly from liquid metal by combining continuous casting with hot rolling in a single step. The thermo-mechanical cyclic interaction with the solidifying strip causes fatigue crack formation at the outer surface of the rolls. A 2D FEM model with Eulerian boundary conditions and the interference fit load on the rolls was defined. The influence of the roll–strip thermal contact, the inlet temperature of the liquid aluminum, the efficiency of the water cooling and the production rate on the fatigue damage of the rolls was analyzed with a parametric study. The maximum temperature of the rolls, the maximum contact pressure, the accumulated plastic strain and the equivalent strain computed (considering a multiaxial out-of-phase fatigue criterion) were considered to investigate the thermo-mechanical fatigue load on the rolls. The results showed that, in the considered range, the most influential parameters on the fatigue mechanism are the heat contact conductance coefficient, which dominates the thermo-mechanical load, and the tangential velocity of the rolls, which contributes to the thermal field and determines the roll–strip mechanical contact interaction.
Information Consumption Habits of Young Colombian University Students
Authors: Barrios-Rubio Andrés
Published: July 2024

The younger generations are exerting a profound influence on the future of the media. The current crisis of reputation facing traditional media is compelling those involved in the creation, production, and distribution of content to rethink the logic underpinning these processes. The consumption of entertainment and information via smartphones is becoming increasingly concentrated in screen devices and social platforms. In light of the particularities of a convergence of media and the characteristics of the digital sphere of users, it is necessary to investigate the information consumption habits and preferences of young university students towards conventional and digital media platforms, as well as social networks within the digital sphere. The objective of this research was to ascertain the news consumption habits of young university students in the context of the new digital landscape, characterised by the pervasiveness of connectivity and the ascendency of smartphones as the primary gateway to the internet. A survey of 1300 students was conducted in October 2023 to investigate their adoption, use, and consumption of traditional and digital media, as well as the devices they use and the importance of podcasts. The results, viewed through the lens of a specific ecosystem, such as that of Colombia, challenge prevailing stereotypes and suggest that younger generations continue to value traditional journalistic brands, potentially due to familial influence. The majority of content is accessed through screen devices and social media platforms. Despite the existence of prejudices, young people supplement their musical preferences with informational content and leisure and entertainment products.
Framing Income Inequality: How the Spanish Media Reported on Disparities during the First Year of the Pandemic
Authors: Odriozola-Chéné Javier , Pérez-Arozamena Rosa
Published: July 2024

This paper addresses the problem of how Spanish digital media reported income inequality during the first year of the COVID-19 pandemic. In this way, the goal was to study the framing of definition, contextual aspects, and depth. For this article, a tool was designed to analyse the content of the items. An analysis of news published by six digital media in Spain from March 2020 to February 2021 was conducted using content analysis. Within a sample of 2727 media stories in which there was a connection between the coronavirus and inequality, a stratified sample was used (n = 958) according to the content production by quarter and by media. The results of this study show that income inequality was the most common type of inequality reported in the media, and they cantered more on the micro level. Also, it appeared to be linked to the social gap and showed poverty as the main consequence. The frame was focused on social issues, international and national contexts, and expert sources. Finally, different levels of depth can be observed in the news items analysed, depending on the frame.
Zinc and Copper Oxide Nanoparticles: Pioneering Antibacterial and Antibiofilm Strategies for Environmental Restoration against Antibiotic-Resistant Bacteria
Authors: Uthra Chandrabose , Nagaraj Karuppiah , Wadaan Mohammad Ahmad , Karuppiah Chelladurai , Maity Prasenjit , Baabbad Almohannad , Kaliyaperumal Raja , Venkatachalapathy Renuka , Shah Flora , Kumar Puneet
Published: July 2024

This study explores the challenge of antimicrobial resistance by investigating the utilization of zinc oxide (ZnO) and copper oxide (Cu2O) nanoparticles (NPs) to combat antibiotic-resistant bacteria in wastewater treatment plants (WWTPs). The synthesized metal oxide NPs underwent thorough characterization through various analytical techniques, confirming their nanoparticulate nature. Electronic absorption and X-ray diffraction (XRD) analyses revealed successful reduction processes and crystalline properties, respectively. Fourier transform infrared spectroscopy (FTIR) results indicated the stabilization of nanoparticles in solution. Scanning electron microscopy (SEM) observations revealed well-defined spherical and flower-like morphologies for the zinc and copper oxide nanoparticles, with sizes approximately ranging from 50 nm to 25 nm Notably, the synthesized nanoparticles exhibited heightened efficacy in impeding biofilm formation, with zinc oxide NPs displaying superior antibacterial activity compared to copper. These findings suggest the promising potential of these nanoparticles in controlling antibiotic-resistant organisms, even following WWTP treatment processes. This research contributes to the ongoing advancements in nanotechnology aimed at combating antibiotic resistance, offering new prospects for the development of effective wastewater treatment strategies.
Impact of Heat Treatment on the Mechanical Properties and Fracture Morphology of Ti555211 Alloy
Authors: Gao Yushe , Xue Xiangyi , Du Yuxuan , Liu Xianghong , Gao Huixian , Wang Jianguo , Xu Junfeng
Published: July 2024

Heat treatment is important for optimizing the strength performance and improving the toughness of titanium alloys. In this study, we investigated the impact of three heat treatment methods (β-annealing, double annealing, and solid-solution and aging treatment) on the mechanical properties and fracture morphology of Ti555211 titanium alloy. The results show that after β-annealing treatment, the alloy retains a high strength, while showing almost no ductility, and no yield strength. The alloy after double annealing has a high elongation rate (~54%) and lower strength. After solid-solution and aging heat treatment, the alloy was able to retain both high strength and a certain degree of ductility. The optimal heat-treatment process is solid-solution treatment at 820 °C/2 h and aging at 560 °C/12 h, which results in a maximum tensile strength of 1404 MPa and an elongation rate of 11%.
Fabrication and Tribological Performance of Self-Lubricating Porous Materials and Composites: A Review
Authors: Kasar Ashish K. , Jose Subin Antony , D’Souza Brian , Menezes Pradeep L.
Published: July 2024

Porous materials have recently attracted significant attention in the aerospace and biomedical fields for addressing issues related to friction and wear. Porous materials are beneficial in applications where continuous lubrication is not feasible or for components that operate under extreme conditions, such as high speeds, elevated temperatures, and heavy loads. The pores can serve as reservoirs for liquid lubricants, which are gradually released during the operation of the components. The tribological properties of these materials depend on their porosity, the lubricants used, and any additional additives incorporated into the porous materials. This review article provides insight into common fabrication techniques for porous materials and examines their tribological performance for all three classes of materials—polymers, metals, and ceramics. Additionally, it discusses design criteria for porous self-lubricating materials by highlighting the critical properties of both the substrate and lubricants.
The Potential of Wire Explosion in Nanoparticle Production in Terms of Reproducibility
Authors: Égerházi László , Szörényi Tamás
Published: July 2024

Aquasols produced by exploding copper wires represent complex systems in which identifying individual colloidal components poses challenges due to broad and multimodal size distributions and varying shares among oxidation states. To evaluate the reproducibility of copper wire explosion, the size distribution of metallic and oxidized colloidal components within the 10–300 nm diameter range was assessed. Classification of each individual particle into bins according to size and chemical composition was accomplished by reconstructing the recorded optical extinction spectra of three sols produced under identical conditions as the weighted sum of the extinction spectra of individual copper and copper-oxide particles, computed using Mie theory. Our spectrophotometry-based component analysis revealed differences in particle number concentrations of the mainly oxidized nanoparticles, corresponding to deviations observed in the ultraviolet portion of the extinction spectra. Notable uniformity was observed, however, in the number of metallic fine particles, consistent with agreement in spectral features in the visible range. Regarding mass concentration, practically no differences were observed among the three samples, with nano-to-fine ratios of copper particles agreeing within 0.45%. Despite the complex processes during explosion leading to limited reproducibility in the ratio of different copper oxidation states, very good reproducibility (54.2 ± 0.7%) was found when comparing the total copper content of the samples to the mass of the exploded copper wire.
Corrosion Behavior of Ferritic 12Cr ODS and Martensitic X46Cr13 Steels in Nitric Acid and Sodium Chloride Solutions
Authors: Nowik Krzysztof , Zybała Rafał , Sztorch Bogna , Oksiuta Zbigniew
Published: July 2024

This paper presents corrosion resistance results of a 12Cr ferritic ODS steel (Fe-12Cr-2W-0.5Zr-0.3Y2O3) fabricated via a powder metallurgy route as a prospective applicant for fuel cladding materials. In a spent nuclear fuel reprocessing facility, nitric acid serves as the primary solvent in the PUREX method. Therefore, fundamental immersion and electrochemical tests were conducted in various nitric acid solutions to evaluate corrosion degradation behavior. Additionally, polarization tests were also performed in 0.61 M of sodium chloride solutions (seawater-like atmosphere) as a more general, all-purpose procedure that produces valid comparisons for most metal alloys. For comparison, martensitic X46Cr13 steel was also examined under the same conditions. In general, the corrosion resistance of the 12Cr ODS steel was better than its martensitic counterpart despite a lower nominal chromium content. Potentiodynamic polarization plots exhibited a lower corrosion current and higher breakdown potentials in chloride solution in the case of the ODS steel. It was found that the corrosion rate during immersion tests was exceptionally high in diluted (0.1–3 M) boiling nitric acid media, followed by its sharp decrease in more concentrated solutions (>4 M). The results of the polarization plots also exhibited a shift toward more noble corrosion potential as the concentrations increased from 1 M to 4 M of HNO3. The results on corrosion resistance were supported by LSCM and SEM observations of surface topology and corrosion products.
Same Organ, Two Cancers: Complete Analysis of Renal Cell Carcinomas and Upper Tract Urothelial Carcinomas
Authors: Vamesu Sorin , Ursica Oana Andreea , Milea Serban Eduard , Deacu Mariana , Aschie Mariana , Mitroi Anca Florentina , Voinea Felix , Pundiche Mihaela Butcaru , Orasanu Cristian Ionut , Voda Raluca Ioana
Published: July 2024

Background and Objectives: Renal cell carcinomas and upper tract urothelial carcinomas are types of malignancies that originate in the kidneys. Each of these examples shows an increasing trend in the frequency and the mortality rate. This study aims to comprehensively define carcinomas by analyzing clinical, paraclinical, and histological aspects to predict aggressiveness and mortality. Materials and Methods: We conducted a retrospective investigation on a group of patients suspected of kidney cancers. Results: We identified 188 cases. We observed a higher mortality rate and older age in individuals with urothelial carcinomas. Anemia, acute kidney injury, hematuria, and perineural invasion were the main risk factors that predicted their mortality. Tumor size in renal cell carcinomas correlates with the presence of necrosis and sarcomatoid areas. Factors that indicate a higher rate of death are older age, exceeding the renal capsule, a lesion that includes the entire kidney, lymphovascular invasion, acute kidney injury, and anemia. Conclusions: Even if they originate at the renal level, and the clinical–paraclinical picture is similar, the histopathological characteristics make the difference. In addition, to these are added the previously mentioned common parameters that can represent important prognostic factors. In conclusion, the characteristics commonly identified in one type of cancer may act as risk factors for the other tumor. The detected data include threshold values and risk factors, making a significant contribution to the existing literature.