bluuebunny/crossref_metadata_2025 huggingface dataset:
A Recursive Trigonometric Technique for Direct Digital Frequency Synthesizer Implementation
Authors: Xing Xing , Melek William , Wang Wilson
Published: December 2024

This paper presents a novel recursive trigonometry (RT) technique for direct digital frequency synthesizer (DDFS) implementations. Traditional DDFS systems on field programmable gate arrays (FPGAs) either require a substantial amount of read-only memory (ROM) space to store reference values or depend on intricate angle rotation functions to approximate trigonometric values. The proposed RT technique offers a DDFS architecture without using the lookup table (LUT) method, and it can enhance signal accuracy and minimize power consumption. The effectiveness of the proposed RT technique has been implemented in a 13.5 kHz 16-bit DDFS with a minimum of 18.91 mW and was tested on a Lattice FPGA. The effectiveness of the proposed RT technology is assessed by using different FPGA platforms in terms of accuracy, hardware resource efficiency, and power consumption, especially in generating cosine waveforms.
A Retrospective Analysis of Automated Image Labeling for Eyewear Detection Using Zero-Shot Object Detectors
Authors: Matuzevičius Dalius
Published: December 2024

This research presents a retrospective analysis of zero-shot object detectors in automating image labeling for eyeglasses detection. The increasing demand for high-quality annotations in object detection is being met by AI foundation models with open-vocabulary capabilities, reducing the need for labor-intensive manual labeling. There is a notable gap in systematic analyses of foundation models for specialized detection tasks, particularly within the domain of facial accessories. Six state-of-the-art models—Grounding DINO, Detic, OWLViT, OWLv2, YOLO World, and Florence-2—were evaluated across three datasets (FFHQ with custom annotations, CelebAMask-HQ, and Face Synthetics) to assess their effectiveness in zero-shot detection and labeling. Performance metrics, including Average Precision (AP), Average Recall (AR), and Intersection over Union (IoU), were used to benchmark foundation models. The results show that Detic achieved the highest performance scores (AP of 0.97 and AR of 0.98 on FFHQ, with IoU values reaching 0.97), making it highly suitable for automated annotation workflows. Grounding DINO and OWLv2 also showed potential, especially in high-recall scenarios. The results emphasize the importance of prompt engineering. Practical recommendations for using foundation models in specialized dataset annotation are provided.
Phase-Only Transmit Beampattern Synthesis Method for Cluttered Environments for Airborne Radar
Authors: Shi Jing , Zeng Cao , Lai Lichu , Zhang Jiaqi
Published: December 2024

In order to solve the problem of strong downward clutter jamming in airborne radar detection, we propose a phase-only transmit beampattern synthesis method. Firstly, with the aim of minimizing the sidelobe gain in the cluttered region, the desired radiation pattern is constructed by using terrain environmental information from where the airborne radar operates. Secondly, an optimization model for phase-only transmit beampattern synthesis accounting for four constraints (the mainlobe gain, the sidelobe gain in the highly cluttered region, the sidelobe gain at other angles, and the amplitude of the weight vector) is established. The Alternating Direction Method of Multipliers (ADMM) is then used to find the iterative solution. Based on the results of four sets of simulation examples designed to verify the effectiveness of the proposed method, it is concluded that the method can reduce the echo intensity in the cluttered region and is suitable for a wide range of array configurations.
Optimization of Bifurcated Switching by Enhanced Synthetic Antiferromagnetic Layer
Authors: Sun Yihui , Meng Fantao , Gong Junlu , Gao Yang , Chen Ruofei , Zhao Lei , Zeng Dinggui , Fu Ting , He Weiming , Wang Yaohua
Published: December 2024

Defects in the free layer are considered to be the main cause of the balloon effect, but there is little insight into the synthetic antiferromagnetic (SAF) layer. To address this shortcoming, in this work, an optimized SAF layer was introduced in the perpendicular magnetic tunneling junction (pMTJ) stack to eliminate the low-probability bifurcated-switching phenomenon. The results indicated that the Hf field in the film stack improved significantly from ~5700 Oe to ~7500 Oe. A magnetoresistive random access memory (MRAM) test chip was also fabricated with a 300 mm process, resulting in a significantly improved ballooning effect. The results also indicated that the switching voltage decreased by 18.6% and the writing energy decreased by 33.7%. In addition, the low-probability stray field along the x-axis was thought to be the main cause of the ballooning effect, and was experimentally optimized for the first time by enhancing the SAF layer. This work provides a new perspective on spin-flipping dynamics, facilitating a deeper comprehension of the internal mechanism and helping to secure improvements in MRAM performance.
Study on the Characteristics of Turn-to-Turn Short Circuit Faults in the Primary Windings of the Generator Terminal Voltage Transformer
Authors: Wei Yang , Wu Ligui , Li Guangyao , Li Chen , Sun Yue , Qiu Pengyu , Qian Yuzhe , Chen Jun
Published: December 2024

Turn-to-turn short circuit faults in the primary winding of generator terminal voltage transformers can lead to erroneous operation of stator grounding protection systems. This paper analyzes the fault characteristics associated with such failures and derives formulas for the fault phase current and zero-sequence voltage during a turn-to-turn short circuit in the primary winding. A 3D finite element model of the generator terminal voltage transformer is established by using Altair Flux 3D, and the accuracy of the model is verified. Based on this model, simulation tests were conducted to investigate turn-to-turn short circuits in the primary winding. The results reveal that as the number of shorted turns increases, the voltage of the fault phase decreases continuously while the voltages of the other two phases increase. The current in the short-circuited phase rises significantly, accompanied by an increase in zero-sequence voltage. Visualizations of magnetic field parameters indicate that as the number of shorted turns increases, the magnetic induction magnitude of the fault phase rises steadily and approaches saturation, resulting in heightened magnetic field intensity near the shorted turns. This analysis of fault characteristics through simulation contributes to the advancement of fault diagnosis systems for generator terminal voltage transformers.
A Mobility Handover Decision Method Based on Multi-Topology
Authors: Zhang Chi , Deng Haojiang , Han Rui
Published: December 2024

With the emergence of new applications in mobile networks, users demand higher network stability and lower data transmission delays. When the network address of a mobile user changes, the data transmission path in the wired network may need to be switched to maintain service continuity. Traditional mobility support methods typically rely on a single switching path for all mobile data flows. However, if this path cannot meet the requirements of all the flows, it may lead to network congestion or a decline in user experience. To overcome this limitation, this paper proposes a mobility handover decision method based on multi-topology. It enables the dynamic allocation of mobile data flows across different switching paths within multiple logical topologies. The method models a multi-topology selection problem aimed at minimizing average packet transmission delay and packet loss rate, while considering network conditions and the Quality of Service (QoS) requirements for each flow. By solving the dual problem of the original optimization, a near-optimal solution is achieved. The proposed scheme and algorithm were implemented and tested using the Mininet network simulator. Results show that the proposed approach achieves low average packet transmission delay, low average packet loss rate, and high throughput, compared to traditional single-path switching methods and existing multipath routing methods.
Applied Research on Face Image Beautification Based on a Generative Adversarial Network
Authors: Gan Junying , Liu Jianqiang
Published: December 2024

Generative adversarial networks (GANs) are widely used in image conversion tasks and have shown unique advantages in the context of face image beautification, as they can generate high-resolution face images. When used alongside potential spatial adjustments, it becomes possible to control the diversity of the generated images and learn from small amounts of labeled data or unsupervised data, thus reducing the costs associated with data acquisition and labeling. At present, there are some problems in terms of face image beautification processes, such as poor learning of the details of a beautification style, the use of only one beautification effect, and distortions being present in the generated face image. Therefore, this study proposes the facial image beautification generative adversarial network (FIBGAN) method, in which images with different beautification style intensities are generated with respect to an input face image. First, a feature pyramid network is used to construct a pre-encoder to generate multi-layer feature vectors containing the details of the face image, such that it can learn the beautification details of the face images during the beautification style transmission. Second, the pre-encoder combines the separate style vectors generated with respect to the original image and the style image to transfer the beautification style, such that the generated images have different beautification style intensities. Finally, the weight demodulation method is used as the beautification style transmission module in the generator, and the normalization operation on the feature map is replaced with the convolution weight to eliminate any artifacts from the feature map and reduce distortions in the generated images. The experimental results show that the FIBGAN model not only transmits the beautification style to face images in a detailed manner but also generates face images with different beautification intensities while reducing the distortion of the generated face images. Therefore, it can be widely used in the beauty and fashion industry, advertising, and media production.
Effects of Bacterial Enzyme Cooperative Fermentation Diet on Growth Performance, Blood Biochemical Indices, and Fecal Microflora of Growing–Finishing Pigs
Authors: Geng Yanchao , Wang Xin , Bao Xinyu , Li Mengting , Gao Yumeng , Qin Shunyi , Yang Hua , Pu Lei , Hong Liang , Zhang Jianbin
Published: December 2024

This research utilized Fourier transform infrared (FTIR) spectroscopy to analyze and discuss the molecular structure of pig diets, aiming to provide new insights into the application of fermented feeds in livestock and poultry production. Moreover, the impacts of the fermented diet on growth performance, apparent digestibility, blood biochemical indices, and fecal microorganisms at different stages of pig fattening were also explored. Forty-eight pigs (Duroc × Landrace × Large white three-way hybrid) with a mean body weight of 16.55 ± 3.88 kg were randomly divided into three groups with four replicates per group and four pigs per replicate. The control group was fed a basal diet. The pigs in the fermented diet group (T1) were fed Pediococcus acidilactici (PA), Lactobacillus reuteri (LR), and Bacillus velezensis (BS) (ratio of 1:1:1) at a 6% inoculation dose. The pigs in the cooperative fermentation group (T2) were fed 6% PA, LR, BS, and a 0.2% compound enzyme preparation. The T1 and T2 diets were fermented with 45% water at 33 °C for 48 h. The pre-feeding period lasted 7 days, and the experimental period lasted 84 days. The experimental results showed that the bacterial enzyme cooperation fermentation process significantly increased the contents of crude protein, calcium, and phosphorus in the diet; increased the area of amide Ⅰ region; increased the apparent digestibility of neutral detergent fiber and phosphorus; significantly increased average daily gain; and decreased the feed-to-gain ratio in the late fattening and growth period. During the whole experiment, the serum concentrations of total protein and immunoglobulin A were significantly increased, the serum concentrations of superoxide dismutase and glucose were decreased, and the diversity and richness of fecal microorganisms were increased. These results show that the bacterial enzyme cooperative fermentation diet can improve the apparent digestibility of nutrients and improve overall health by increasing the area of amide Ⅰ region.
Enhanced Multi-Stress Tolerance in Escherichia coli via the Heterologous Expression of Zymomonas mobilis recA: Implications for Industrial Strain Engineering
Authors: Phannarangsee Yupaporn , Kitwetcharoen Haruthairat , Thanonkeo Sudarat , Klanrit Preekamol , Yamada Mamoru , Thanonkeo Pornthap
Published: December 2024

This study investigated the role of the Zymomonas mobilis recA gene in conferring stress resistance when expressed in Escherichia coli. The recA gene was cloned and expressed in E. coli BL21(DE3), producing a 39 kDa polypeptide. The results of comparative analyses demonstrated that the recombinant strain significantly enhanced survival rates under various stress conditions. In oxidative stress tests, the recombinant E. coli pET-22b(+)-recA exhibited superior survival at 3 mM and 5 mM H2O2 concentrations. Heat stress experiments at 50 °C and 55 °C revealed increased survival for the recombinant strain. Under ethanol stress, particularly at 20% (v/v), E. coli pET-22b(+)-recA displayed higher viability than controls. UV-C exposure tests further highlighted the protective effect of recA expression, with the recombinant strain maintaining viability after 60 min of exposure, while control strains showed no survival. These results indicate that the Z. mobilis recA gene product enhances resistance to oxidative, heat, ethanol, and UV-C stresses when expressed in E. coli. This study elucidates the broad stress-protective functions of the RecA protein across bacterial species and suggests potential applications in developing stress-tolerant bacterial strains for biotechnological purposes.
A Potential Diabetic-Friendly Food Material: Optimization, Nutritional Quality, Structural Characteristics, and Functional Properties of Oat and Purple Potato Fermented by Ganoderma lucidum Mycelium
Authors: Guan Yingxian , Zhao Tong , Zhang Anrong , Zhang Di , Huang Xiaoxiao , Fang Xiao , Geng Jiajun , Gang Jie
Published: December 2024

Dietary intervention is the basis for the treatment of diabetes mellitus. This study employed Ganoderma lucidum (GL) mycelium to ferment a compound medium of oat and purple potato (OPP), optimized fermentation conditions to increase the triterpene content in the resulting product (F-OPPF), and systematically investigated the impact of fermentation on the nutritional quality, structural characteristics, and functional properties of OPP. The results indicated that the triterpene content in F-OPPF significantly increased from 8.53 mg/g to 17.23 mg/g under optimal conditions (temperature: 28 °C, inoculum size: 10%, material quantity: 36 g/250 mL, and fermentation time: day 13). Fermentation resulted in enhanced nutritional quality, with increased contents of protein, soluble protein, crude fiber, ash, mineral elements, essential amino acids, polysaccharides, flavonoids, and total phenols. Mycelium not only enveloped the OPP surface but also penetrated its interior, forming a porous honeycomb-like structure. The types of reactive groups and crystals (C + V-type) were not changed after fermentation, while the crystallinity increased. F-OPPF exhibited positive changes in thermogravimetric properties, antioxidant and hypoglycemic activities, and adsorption capacity of insoluble dietary fiber. Additionally, incorporating F-OPPF into the diet markedly reduced fasting blood glucose levels and promoted weight gain in T2DM rats induced by a high-fat diet and streptozotocin. The fermented groups exhibited improvements in glyco- and lipo-metabolism, oxidative stress, and the function and pathological morphology of the pancreas, liver, and kidneys compared to the unfermented group. Collectively, these findings suggested that GL mycelium fermentation enhanced the nutritional and functional values of OPP, and F-OPPF holds potential as a raw material for developing diabetic-friendly foods.
Continuous Cultivation of Yarrowia lipolytica: Potential, Challenges, and Case Studies
Authors: Kamzolova Svetlana V.
Published: December 2024

Currently, the yeast Yarrowia lipolytica is regarded as one of the most promising producers of protein, lipids, polyols, organic acids, and other metabolites. The objective of enhancing the efficiency of the target product biosynthesis can be achieved through the improvement of the strains-producers and the optimization of the cultivation conditions. The present review assesses the potential of continuous cultivation methods (chemostat, turbidostat, pH-auxostat, changestats, etc.) in order to gain insight into the impact of strains and cultivation conditions on the productivity of the developed bioprocesses. The utilization of continuous cultivation methods enables the implementation of processes under controlled and reproducible conditions, thus stabilizing the parameters of the cultivation and the physiological state of the producer, and obtaining homogeneous samples. The review focuses on nitrogen-limited chemostat cultures, which represent the most commonly employed strategy for investigating the physiological and biochemical characteristics of the yeast Y. lipolytica and for developing the processes for the production of lipids, erythritol, citric acid, and recombinant proteins. To date, such an analysis of the literature has not been conducted in the context of the yeast Y. lipolytica.
Advances in Blockchain-Based Internet of Vehicles Application: Prospect for Machine Learning Integration
Authors: Okere Emmanuel Ekene , Balyan Vipin
Published: December 2024

Blockchain-based technology has completely revolutionized the development of the Internet of Vehicles (IoV) framework. This has led to increasing blockchain-based Internet of Vehicles application over the last decade. However, challenges persist, including scalability, interoperability, and security issues. This paper first presents the state-of-the-art overview on IoV systems along with their applications. Then, we explore novel technologies, including blockchain-based IoV and machine learning-based IoV and highlight how the blockchain technology could be integrated with machine learning for intelligent transportation systems in the IoV ecosystem. This paper has shown the potential of machine learning integration in addressing the technical challenges in individual blockchain-based Internet of Vehicles applications.
Comprehensive Bibliometric Review on the Sustainability and Environmental Impact of Fiber-Reinforced Polymers
Authors: Tănase Maria , Diniță Alin , Popovici Daniela Roxana , Portoacă Alexandra Ileana , Călin Cătălina , Sirbu Elena-Emilia
Published: December 2024

Fiber-reinforced polymers (FRPs) are increasingly recognized in sustainable materials research due to their potential environmental advantages. This study presents a focused bibliometric review of the sustainability research on FRPs. An initial search of the Web of Science (WOS) database identified 803 documents, which were refined to 749 relevant articles, reviews, and proceedings. A co-authorship analysis highlights the significant contributions of the USA and India, with European countries forming regional collaborations. The research output has steadily increased since 2011, peaking in 2022 and 2023. The multidisciplinary nature of the research spans materials science, engineering, and environmental sciences, with journals such as *Polymers*, *Sustainability*, and the *Journal of Cleaner Production* emphasizing sustainability themes. This analysis covers key aspects such as keyword co-occurrence, overlay visualizations, co-authorship networks, and the distribution of publications by year, research area, and journal. The findings underscore the evolving research landscape of sustainable FRPs and highlight the ongoing need for life cycle assessments and interdisciplinary collaboration.
Selection of Reference Genes by Quantitative Real-Time PCR in Different Cell Lines from Humpback Grouper (Cromileptes altivelis)
Authors: Du Xiangyu , Zhang Han , Zhu Longfei , Cao Zhenjie , Zhang Chen , Wu Ying , Zhou Yongcan , Sun Yun
Published: December 2024

Humpback grouper (Cromileptes altivelis) is an economically important fish, but the increasing density of its farming has led to more severe disease outbreaks. To address this challenge, we established brain (CAB) and kidney (CAK) cell lines in our laboratory previously, providing a valuable tool for in vitro studies on immune responses. In this study, we used quantitative real-time polymerase chain reaction (qRT-PCR) to identify the optimal reference gene from six reference genes for CAB and CAK cells, under both normal conditions and after stimulation with LPS or Poly I: C. The qRT-PCR data were analyzed using geNorm, NormFinder, and BestKeeper software (Version 3.5) to ensure comprehensve evaluation. The results showed that RPL13 was the most stable reference gene for both CAB and CAK cells under normal conditions. Following LPS stimulation, TTLL1 was the best reference gene for CAB cells, while RPL13 remained the most suitable for CAK cells. For Poly I: C stimulation, EF1A and Actin were identified as the most stable reference genes for CAB and CAK cells, respectively. To confirm the reliability of the selected reference genes, we analyzed the expression of the cytokine genes IL-6 and IFN-h, demonstrating the dependability of these reference genes. This study lays a solid foundation for exploring gene expression patterns in humpback grouper cell lines under various experimental conditions, providing essential insights for future research into immune processes and disease control strategies in aquaculture.
The Oxytetracycline and Florfenicol Effect on the Immune System and Oxidative Stress Response of the SHK-1 Cell Line of Salmo salar
Authors: Vargas-Chacoff Luis , Figueroa Daniela , Nualart Daniela , Muñoz José Luis
Published: December 2024

The aquaculture industry in Chile, as in the rest of the world, has rapidly grown, becoming a crucial economic sector. However, diseases pose a major threat, causing significant economic losses and environmental impacts. Various antimicrobials, particularly Oxytetracycline and Florfenicol, are used to combat these diseases, which has boosted production and mitigated economic losses. However, excessive antibiotic use has led to pathogen resistance, necessitating higher doses. This overuse can cause side effects in fish, including liver damage and immunosuppression. This study aimed to determine the impact of multiple doses of florfenicol and oxytetracycline on the SHK-11 cell line of Salmo salar by analyzing the expression of genes related to innate immunity and oxidative stress by qRT-PCR in addition to the quantification of immune system proteins via dot blot. The experimental treatments were the following: cells were stimulated with different concentrations of oxytetracycline (0.25, 0.5, and 1.5 µg/mL) and florfenicol (1, 10, and 20 µg/mL) for time kinetics of 0.5, 1, 3, 6, 12, 24, and 48 h. For both cases, controls consisting of cells without antibiotics were included. The expression of the immune system genes was mostly inhibited compared to the control. However, it was observed that TLR-1 and MyD88 present a joint activation pattern at different times and concentrations for both antibiotics. Regarding the expression of CAT and GPx, transcripts were increased in the early stages of stimulation with oxytetracycline and florfenicol, followed by a subsequent decrease in gene expression. This study provides relevant information to understand the effect of antibiotics at the cellular level in one of the most important species for global aquaculture, the Atlantic salmon.
It’s Time for Dinner, a Particular and Seasonal Feeding Habit of a Threatened Troglobitic Catfish from Brazil, Rhamdiopsis krugi Bockmann & Castro 2010 (Ostaryophysi, Siluriformes)
Authors: Bichuette Maria E.
Published: December 2024

Rhamdiopsis krugi is a highly specialized troglobitic (exclusively subterranean) catfish endemic to the phreatic water bodies of twelve caves located within two separated metasedimentary basins in the region of Chapada Diamantina, Bahia state, Brazil. This species is included in the List of Endangered Fauna of Brazil, under the Vulnerable category—VU. In general, troglobites have different strategies for searching for food and reproductive partners, as well as unique behaviors. Knowledge of the reproductive periods, as well as its feeding habits, provides fundamental data for effective protection and species conservation. Biological aspects related to feeding habits and reproduction of R. krugi were addressed across six annual cycles, considering both dry and rainy seasons. For this, stomach content analysis, using the frequency of occurrence and volumetric index methods, as well as observation of the sex ratio and stage of maturation of the gonads were carried out for 148 individuals of R. krugi sampled in eight caves in Chapada Diamantina. Stomach volumes correlated with reproduction aspects across the dry and rainy seasons. These populations showed opportunistic carnivorous feeding habits, consuming both autochthonous and allochthonous items, with a preference for foraging in submerged guano deposits, which demonstrates the catfish’s strong dependence on bats. Regarding sex ratios, there was no marked seasonality; however, in rainy seasons, there was a higher proportion of maturing females, showing a reproductive tendency. During these periods, there was also a significantly higher number of stomachs with contents, showing seasonality in the diet. Specialized diet and dependence on rainy periods, especially in diet, corroborate the fragility of R. krugi, especially considering the changes in rainfall regimes in Brazil, with dry seasons exceeding eight months per year in the last ten years.
Estimation of Dietary Exposure to Sodium Benzoate (E211) and Potassium Sorbate (E202) of Children and Adolescents in the Oran Region, Algeria
Authors: Zouaoui Fatima Saida , Boukhari Rachid , Heroual Nabila , Djemouai Nadjette , Redouane Dalal , Saidi Djamel , Addou Samia , Kheroua Omar
Published: December 2024

Sodium benzoate (E211) and potassium sorbate (E202) are two preservatives widely used alone or in combination in the food industry in Algeria. This study aims to estimate the dietary exposure of children and adolescents to these substances in Oran Province (Algeria) and to assess the risks incurred in consuming these two preservatives. For this, a total of 116 commercial food samples were analyzed for potassium sorbate and sodium benzoate content by HPLC, and a survey was carried out on 250 children and 765 adolescents using a consumption frequency questionnaire as the method. The concentration of preservatives in foodstuffs was mostly within the maximum permitted limits set by national and international standards. In scenario 2 and scenario 3, the estimated average dietary exposure to the two preservatives was below the ADI: it was 17–48% and 21–36% of the ADI for potassium sorbate and sodium benzoate, respectively. The dietary exposure at the 95th percentile exceeded the ADI for sodium benzoate and potassium sorbate when calculations were made using the average of samples containing these preservatives. The main contributors to exposure were carbonated drinks for sodium benzoate and juices, cheeses, and yogurts for potassium sorbate.
Investigating the Effects of Acid Hydrolysis on Physicochemical Properties of Quinoa and Faba Bean Starches as Compared to Cassava Starch
Authors: Halim Anthony , Torley Peter J. , Farahnaky Asgar , Majzoobi Mahsa
Published: December 2024

In response to the growing demand for high-quality food ingredients, starches from underutilised sources like quinoa and faba bean are gaining attention due to their unique properties and high tolerance to adverse environmental conditions. Acid hydrolysis is a well-established chemical method for producing modified starch with improved solubility, lower gelatinisation temperature, and reduced pasting viscosity. However, various outcomes can be achieved depending on the type of starch and modification conditions. This study comparatively investigated the effects of acid hydrolysis on the functional and physicochemical properties of emerging starches from quinoa and faba bean, with cassava starch serving as a reference from a leading source. The results demonstrated increased dietary fibre content across all three starches, with faba bean starch showing the most significant rise. Acid treatment also enhanced the crystallinity of the starches, with faba bean starch exhibiting the highest increase in relative crystallinity, which led to a shift towards higher temperatures in their thermal properties. Additionally, water solubility and oil adsorption capacity increased, while swelling power decreased following acid treatment. The acid treatment reduced the pasting properties of all samples, indicating that the modified starches were more resistant to heating and shearing in the rapid visco analyser. While quinoa starch gel remained soft after acid hydrolysis, the gel strength of cassava and faba bean starches improved significantly, making them suitable as plant-based gelling agents.
Resistant Starch Type 5 Formation by High Amylopectin Starch–Lipid Interaction
Authors: Castro-Campos Fernanda G. , Esquivel-Fajardo Edgar A. , Morales-Sánchez Eduardo , Rodríguez-García Mario E. , Barron-Garcia Oscar Yael , Ramirez-Gutierrez Cristian Felipe , Loarca-Piña Guadalupe , Gaytán-Martínez Marcela
Published: December 2024

The formation of resistant starch type 5 (RS5), primarily associated with amylose–lipid complexes, is typically attributed to starches with high-amylose content due to their affinity for lipid interactions. Recently, studies have also investigated the potential of amylopectin-rich starches to form amylopectin–lipid complexes (ALCs), expanding RS5 sources. This study explores the capacity of waxy corn starch (WS), which is rich in amylopectin, to develop ALCs with oleic acid (10% w/w) under different thermal and mechanical conditions. Specifically, WS was treated at temperatures of 80 °C, 85 °C, and boiling, with stirring times of 0 and 45 min. Results demonstrated significant ALC formation, reaching a peak complexation index (CI) of 59% under boiling conditions with 45 min of stirring. Differential scanning calorimetry (DSC) identified a notable endothermic transition at 110 °C, indicating strong ALC interactions. FTIR spectra further evidenced starch–lipid interactions through bands at 2970 cm−1 and 2888 cm−1. X-ray diffraction (XRD) analysis confirmed the presence of orthorhombic nanocrystals in native WS, with ALC samples exhibiting a V-type diffraction pattern, supporting effective complexation. This study advances knowledge on starch–lipid interactions, suggesting ALCs as a promising RS5 form with potential food industry applications due to its structural resilience and associated health benefits.
A Comparative Study of Physicochemical, Aroma, and Color Profiles Affecting the Sensory Properties of Grape Juice from Four Chinese Vitis vinifera × Vitis labrusca and Vitis vinifera Grapes
Authors: Yang Chen , Fan Xinyue , Lao Fei , Huang Jin , Giusti M. Monica , Wu Jihong , Lu Hongmei
Published: December 2024

In order to compare the grape juice sensory properties of four common seedless grape varieties (Kyoho, Summer Black, Moldovan, and Sweet Sapphire) in China, a thorough comparison of these grape varieties was conducted. Physicochemical indicators, aroma, and color characteristics were analyzed and compared to a commercially available not-from-concentrate Concord grape juice. The contents of fructose, glucose, and seven organic acids were evaluated. Kyoho and Sweet Sapphire possessed optimal Brix–acid ratio in the range around 35–40. In terms of aroma, 60 volatiles were identified by GC-MS, including 16 alcohols, 9 terpenes, 6 aldehydes, and 4 ketones. Kyoho demonstrated the highest aroma intensity with superior floral and fruity notes, while Sweet Sapphire showed the lowest aroma intensity with a grassy scent. Additionally, grape pigment profiles were investigated by HPLC-PDA-MS. Summer Black grapes featured a vibrant color, and 52.5% of their anthocyanins were acylated, which helps provide good stability in follow-up processing. Concord juice showed the best overall properties, with the most saturated color and delightful aroma. It was suggested that blending Summer Black with Kyoho could be a promising way to achieve balanced color, taste, and aroma of grape juice. This study provides a feasible and promising combination of Chinese local grapes for making all-round high-quality juice products.
How Argentinian Consumers Perceive the Safety of Irradiated Foods
Authors: Rusin Tiago , Villavicencio Anna Lucia Casañas Haasis , Araújo Wilma Maria Coelho , Faiad Cristiane
Published: December 2024

Food irradiation is a process used for various purposes, the main function of which is food safety. Although food irradiation has been used to ensure food safety, most consumers are unaware of the basic concepts of irradiation, misinterpreting information and showing a negative perception towards food treated with ionizing radiation. This research aimed to develop the cross-cultural adaptation and validation of the Awareness Scale on Consumption of Irradiated Foods (ASCIF) for the Argentine population and culture. The scale included 31 items covering 4 factors: safety of irradiated foods (S), concepts (C), labeling (L), and awareness (A), which were able to assess the Argentine population’s knowledge of irradiated foods. The total number of respondents was 500 and the data were collected by means of an electronic survey. Statistical tests were carried out which met the validity assumptions and confirmed the validity and consistency of the psychometric scale by means of confirmatory factor analysis (CFA), Cronbach’s alpha coefficient, and exploratory structural equation modelling (ESEM). Analysis of the results showed that the majority of consumers are unaware of the benefits of irradiated foods. It was found that the scale met the criteria for evidence of validity and consistency, proving to be an efficient tool for assessing potential challenges and opportunities in the Argentinian market for irradiated foods. The process was approved by the Research Ethics Committees of Brazil and Argentina and followed the adaptation methodologies of the International Test Commission (ITC) with processes of translations and retranslations and application of the scale in Argentina.
Advances in Vibrational Spectroscopic Techniques for the Detection of Bio-Active Compounds in Virgin Olive Oils: A Comprehensive Review
Authors: Ding Fangchen , Sánchez-Villasclaras Sebastián , Pan Leiqing , Lan Weijie , García-Martín Juan Francisco
Published: December 2024

Vibrational spectroscopic techniques have gained significant attention in recent years for their potential in the rapid and efficient analysis of virgin olive oils, offering a distinct advantage over traditional methods. These techniques are particularly valuable for detecting and quantifying bio-active compounds that contribute to the nutritional and health benefits of virgin olive oils. This comprehensive review explores the latest advancements in vibrational spectroscopic techniques applied to virgin olive oils, focusing on the detection and measurement of key bio-active compounds such as unsaturated fatty acids, phenolic compounds, and other antioxidant compounds. The review highlights the improvements in vibrational spectroscopy, data processing, and chemometric techniques that have significantly enhanced the ability to accurately identify these compounds compared to conventional analytical methods. Additionally, it addresses current challenges, including the need for standardized methodologies and the potential for integrating vibrational spectroscopy with other analytical techniques to improve accuracy and reliability. Finally, findings over the last two decades, in which vibrational spectroscopy techniques were effectively used for the detailed characterization of bio-active compounds in virgin olive oils, are discussed.
Sustainable Design and Evaluation of Children’s Food Packaging from the Perspective of Buyers’ Preferences
Authors: Xiao Ying , Wang Yihan , Wei Yangyang
Published: December 2024

Consumer behavior is one of the key factors influencing product sales, especially in food packaging design, where green, organic, sustainable, and human-centered designs are more effective in promoting food sales. This paper aims to develop a sustainability evaluation method for children’s food packaging. The study first explores the theoretical foundations of sustainability, establishing a systematic set of quantitative indicators and evaluation criteria. Based on this framework, the research gathers consumption behavior, rating data from 250 parents of various ages, professions, and income backgrounds. Using the CRITIC model, the study performs dimensionless processing and detailed quantitative evaluation of the indicators’ comparability, contradictions, and information content to allocate weights for the sustainability evaluation metrics. Furthermore, the MABAC model is applied to construct a weighted decision matrix and boundary approximation area, ranking the sustainability of 20 representative children’s food packaging design schemes (S1–S20). The results show that Scheme S1, after calculation using the CRITIC–MABAC model, has a total distance of 0.214 from the boundary approximation area, exhibiting the smallest deviation from the ideal solution across multiple evaluation criteria and achieving the best overall performance. Building on the optimal Scheme S1, this study comprehensively considers key elements such as eco-friendliness, safety, functionality, and educational value in the optimization of a sustainable design for children’s fruit puree packaging. The research validates the practicality and effectiveness of the quantitative model through the sustainable design and evaluation of children’s food packaging from a consumer behavior perspective, promoting sustainability design and optimization in the children’s food packaging sector.
Assessment of CPME as Sustainable Low VOC Alternative to Hexane: Optimization of Extraction Efficiency and Bioactive Compound Yield from Fenugreek Seed Oil Using Computational and Experimental Methods
Authors: Ben Abdennebi Ameni , Chaabani Emna , Ben Jemaa Mariem , Hammami Majdi , Khammassi Saber , Nait Mohamed Salma , Aidi Wannes Wissem , Hamrouni Sellami Ibtissem , Fabiano Tixier Anne-Sylvie , Bettaieb Rebey Iness
Published: December 2024

This study investigates the performance of cyclopentyl methyl ether (CPME) in the extraction of fenugreek seed oil, aiming to replace the conventionally used hexane. The efficiency of this alternative solvent was evaluated first through in silico methods (based on Hansen Solubility Parameters (HSPs) and Conductor-like Screening Model for Real Solvent (COSMO-RS) simulations), followed by experimental studies. Solubility computational predictions analysis revealed that CPME exhibits superior solvation power compared to hexane. Experimentally, CPME demonstrated a significantly higher oil yield (7.23%) compared to hexane (4.25%) and a better retention of beneficial unsaturated fatty acids than hexane. Additionally, the physicochemical properties of oils extracted with CPME showed enhanced oxidative stability, sterol, tocopherol, and phenolic contents, leading to superior antioxidant and antibacterial activities. Importantly, CPME’s low volatile organic compound (VOC) emissions further establish it as a more sustainable and environmentally friendly alternative to hexane, aligning with contemporary goals of reducing harmful emissions in extraction processes. Thus, this paper highlights the functional advantages of CPME, focusing on its efficiency, selectivity, and enhanced retention of bioactive compounds, positioning it as a superior extraction solvent for fenugreek seed oil compared to hexane.
Amino Acid Composition of Dried Bovine Dairy Powders from a Range of Product Streams
Authors: Gilmour Simon R. , Holroyd Stephen E. , Fuad Maher D. , Elgar Dave , Fanning Aaron C.
Published: December 2024

The amino acid (AA) content of multiple samples of various dairy powders was determined, providing a comprehensive evaluation of the differences in AA profiles attributable to distinct manufacturing processes. Products examined included whole milk powder (WMP), skim milk powder (SMP), cheese whey protein concentrate (WPC-C), lactic acid casein whey protein concentrate (WPC-L), high-fat whey protein concentrate (WPC-HF), hydrolyzed whey protein concentrate (WPH), whey protein isolate (WPI), and demineralized whey protein (D90). WMP and SMP exhibited broadly similar AA profiles, with minor differences likely due to the minimal milk fat protein content, which is nearly absent from SMP. Comparative analysis of WPC-C and WPC-L indicated higher levels of threonine, serine, glutamic acid, and proline in WPC-C but lower levels of tyrosine, phenylalanine, and tryptophan, attributed to the different methods of separation from casein proteins. WPI and WPC-HF originate from similar sweet whey streams but follow divergent processing methods; consequent on this were variations in the levels of all AAs except histidine. The nanofiltration step in D90 production retains its non-protein nitrogen content and affects its AA profile; consequently, D90 consistently exhibited lower AA levels than WPC-C. These findings underscore the significant impact of manufacturing processes on dairy powder AA composition.
Effect of Organic Acid Addition Before Fermentation on the Physicochemical and Sensory Properties of Cherry Wine
Authors: Yang Wenbo , Lv Zhenzhen , Liu Hui , Zhang Qiang , Qiao Chengkui , Nawaz Muhammad , Jiao Zhonggao , Liu Jiechao
Published: December 2024

Lack of acidity is the main reason for the spoilage of cherry wine, and for insufficient aroma and mouthfeel. In this study, the initial acidity of cherry purees was adjusted to 3.50, 4.15, 4.80 and 5.45 g/kg by using malic acid, lactic acid and a mixture of the two before fermentation. And the effects of different organic acid additions on the physicochemical profiles and sensory properties of cherry wines were investigated. Our findings suggest that organic acid addition can inhibit the formation of volatile acid and enhance ethanol production, while having a negative effect on their polyphenol contents. These additions can be utilized as carbon sources during cherry wine fermentation and affect its metabolism. Among them, the application of malic acid with lactic acid was shown to have more metabolically active effects on non-volatile compounds, and enhanced the total volatile organic compounds by 14.04%–66.92%. MC-4.80 and MLC-4.80 had the highest total VOC content and odor score in the sensory evaluation. However, the addition of large amounts of acids reduced the acidity score and overall acceptability of cherry wine. In conclusion, adjusting the initial acid content to 4.15 g/kg before fermentation significantly improved the quality of cherry wines, and the combination of malic acid and lactic acid was more effective for cherry winemaking. This finding evidenced that organic acid addition could be an effective strategy for improving the quality of cherry wines.
Essential Oils Against Spoilage in Fish and Seafood: Impact on Product Quality and Future Challenges
Authors: Pierozan Matheus Barp , Oliveira Filho Josemar Gonçalves de , Cappato Leandro Pereira , Costa Adriano Carvalho , Egea Mariana Buranelo
Published: December 2024

The preservation of fish and seafood represents a significant challenge for the food industry due to these products’ high susceptibility to microbial spoilage. Essential oils (EOs), classified as Generally Recognized as Safe (GRAS), have become a natural alternative to synthetic preservatives due to their antimicrobial and antioxidant properties. This review aims to analyze the specific potential of EOs in extending the shelf life of fish and seafood products, offering a natural and effective preservation solution. It provides a detailed overview of EOs applications and mechanisms, highlighting their role in controlling spoilage microorganisms while maintaining product quality. The main methods of EOs application include immersion, spraying, and pipetting, with antimicrobial effectiveness influenced by factors such as concentration, exposure time, and food characteristics like chemical composition and biofilms. Direct EOs application shows challenges that can be countered by exploring nanoemulsion technology as an effective strategy to enhance EOs stability and controlled release, maximizing their preservation impact. Additionally, coatings made from chitosan, gelatin, Farsi gum, and carrageenan, combined with EOs such as oregano, clove, and thyme have shown efficacy in preserving species like rainbow trout, mackerel, and shrimp. However, the commercial feasibility of using EOs in fish preservation depends on consumer acceptance and regulatory compliance. This review offers valuable insights for the industry and researchers by highlighting the practical applications and commercial challenges of EOs in seafood products, underscoring the importance of consumer acceptance and regulatory adherence for market viability.
The Impact of Artificial and Natural Additives in Meat Products on Neurocognitive Food Perception: A Narrative Review
Authors: Ciobanu Marius-Mihai , Flocea Elena-Iuliana , Boișteanu Paul-Corneliu
Published: December 2024

The holistic sensory experience creates a unified perception that influences consumer memory. Consumer interest in clean label products underlines an accelerated trend towards products without artificial additives. From a sensory point of view, food appeal is significantly influenced by how additives actively participate in the organoleptic properties of the final product. This research aims to shed light on the impact of artificial and natural additives in meat products on neurocognitive food perception, which is essential for understanding how they influence the consumer’s final decision and direct food trends. Different neural mechanisms involved in multimodal sensory integration confirm differences in perception of meat products containing artificial and natural additives. Analysis confirms that neurocognitive perception integrates organoleptic sensations to form a complete sensory experience. The encephalon simultaneously processes multimodally integrated stimuli from organoleptic properties, reaching the orbitofrontal cortex and other regions involved in the neuroprocessing of the final product. The reformulation and development of meat products need a detailed analysis of the impact of additives on sensory properties contributing to the shaping of consumption trends.