bluuebunny/crossref_metadata_2025 huggingface dataset:
DEVELOPMENT OF COORDINATION FORMS OF ORGANIZATION OF TERRITORIAL AND SECTORAL INTERACTIONS IN RURAL AREAS
Authors: Gazetdinov Shamil , Kovalenko Elena , YAkimova Olga , Ziganshin Bulat
Published: April 2023

The prepared article reflects the results of a study of the forms of organizing the coordination of territorial-sectoral interactions in rural areas. A trend of steady decline in the number of rural population and a decrease in the number of rural settlements has been revealed. The results obtained showed that despite the growth in agricultural production noted in recent years, the level and quality of life of the rural population as a whole lag significantly behind the standard of living in cities, and at the same time it is difficult to involve agricultural enterprises, integrated formations and other business entities in solving social problems - economic tasks of the development of territories by administrative methods without taking into account their own economic interests and opportunities. The level of wages in agriculture remains low compared to other sectors of the economy. During the study period, the level of employment in agriculture in the Russian Federation decreased by 10%. In order to overcome the negative phenomena, it is proposed to bring the management system of the integrated socio-economic development of rural areas into line with modern requirements, make it more democratic and efficient, for which it is necessary to create qualitatively different organizational conditions for the joint activities of enterprises and other business entities located in the same territory, using elements of cooperation and integration that have justified themselves in practice, progressive domestic and foreign experience. At the same time, the basis for the formation of territorial-economic associations should be, first of all, the need and interest in organizing joint activities, the expediency and profitability of combining the resources and production potentials of several business entities for this. One of the forms is the formation of a territorial-economic association for the socio-economic development of rural areas on the basis of a voluntary association of enterprises and business entities, both industrial and non-industrial, and municipal governments on the scale of a rural area.
Features of ultrasound and dopplerometric endometry examination in autoimmune thyroiditis patients with abnormal uterine bleeding
Authors: Alieksieieva O. , Lazurenko V. , Vasylieva I. , Afanasiev I. , Kudin I. , Harkavenko K.
Published: April 2023

Abnormal uterine bleeding  occurs in 10–30% of reproductive age women. Ultrasound examination is one of the most effective methods of diagnosing lesions of the pelvic organs while it has high sensitivity (96%), but low specificity (13.8%) in relation to endometrial lesions, the informativeness of ultrasound in the diagnosis of abnormal uterine bleeding ranges from 60 to 93.3%. It had been revealed revealed that the imbalance of thyroid hormones can have a direct effect on the endometrium. Thus, the analysis of ultrasound examinations data  together with dopplerometric assessment of the blood flow of the internal genital organs in autoimmune thyroiditis patients with abnormal uterine bleeding is an urgent task of modern gynecology. The purpose is to determine the features of ultrasound exanimation with Doppler assessment of the endometrium  in autoimmune thyroiditis patients with abnormal uterine bleeding. Materials and methods.  The patients’ examinations were carried out from 2019 to 2022 We examined 120 patients, of whom 90 (75%) suffered from abnormal uterine bleeding and 30 (25%) did not have signs of gynecological and somatic pathology (control group). Abnormal bleeding patients average age was 36.3±3.2 years. The average age of bleeding and thyroid pathology women was 34.8±2.6 years, the control group average age was 30.7±2.9 years. Results. Echographic signs of endometrium structural changes were detected in 83 (92.2%) of abnormal nautoimmune bleeding women and in 7 (7.8%) women ultrasound signs of pathology were not detected. Significant differences were found when analyzing the results of ultrasonographic examination of patients with bleeding and bleeding + thyroid pathology. Conclusion. Ultrasound is an important method that contributes to a significant improvement in the diagnosis of isolated or combined benign uterine diseases in women with abnormal autoimmune bleeding at the background of thyroid pathology, and in most cases corresponds to the histological diagnosis.
Monoclonal antibodies blocking CGRP for prevention of migraine
Authors: Maziarczyk Alicja , Miazga Dominika , Surdacka Laura
Published: April 2023

Introduction and purpose

Monoclonal antibodies blocking calcitonin gene-related peptide (CGRP) are a novel treatment strategy developed specifically for prevention of migraine. Four drugs belong to this group: eptinezumab, fremanezumab and galcanezumab, which bind to the peptide; and erenumab, which blocks the CGRP receptor. CGRP is involved in nociception and plays a crucial role in the pathophysiology of migraine, as it is released in the trigeminal ganglion as a response to local cerebral vasoconstriction in order to cause dilation of the vessels and maintain cerebral blood flow. Moreover, administration of CGRP, especially among migraineurs, induces a migraine-type headache. The aim of the paper is to discuss the potential of monoclonal antibodies blocking CGRP for the prevention of migraine and to outline their safety and efficacy profile.

State of knowledge

Several randomised clinical trials have shown a significant efficacy of these drugs compared to placebo in reducing monthly migraine affected days, among patients suffering from both episodic and chronic migraine. Incidence rate of side effects is low; the most common were mild to moderate (e.g. pain at the injection site, upper respiratory tract infections, nasopharyngitis, back pain and urinary tract infection). Anti-CGRP monoclonal antibodies exhibit a superior benefit-to-risk ratio than established preventive treatments.

Conclusions

Randomized controlled trials are still needed in order to compare different anti-CGRP monoclonal antibodies and asses their long-term safety profile. In conclusion, these drugs seem to provide promising prospects of improving the lives of migraineurs. As based on current knowledge, the benefits are superior to the likelihood of harm.
Mindfulness based interventions in the treatment of obesity
Authors: Winiarz Aleksandra , Wokurka Wojciech , Turek Michał , Szlendak Paula , Toś Katarzyna , Wojtala Kacper , Grosman Sylwia , Węgrzyniak Agata , Drobek Dominik , Wąsala Katarzyna
Published: April 2023

Introduction: Obesity is a chronic, multifactorial disease that leads to many complications. Many methods of treating obesity are available, but they are often not effective enough. Due to the strong connection between psychological factors and the development of obesity, it can be useful to include mindfulness training in treatment. 
The aim of this study: Summarize current knowledge on the effectiveness of mindfulness training in the treatment of obesity and evaluate the effect of mindfulness on weight loss. 
Material and method: The review includes publications published in 2012-2022. The search of publications in the Pubmed database was carried out using the following keywords: mindful eating, mindfulness, weight loss, obesity.
Description of the stage of the knowledge: The use of mindfulness techniques in the treatment of eating disorders is becoming more popular. A growing number of studies evaluating the effects of mindfulness on weight loss are emerging, but the results are inconclusive. Mindfulness training is effective in improving eating habits and promoting lifestyle changes. Mindfulness training combined with behavioral treatments for obesity has positive effects on weight loss.
Summary: Research shows that mindfulness training may be a promising intervention in obesity treatment because it increases self-awareness and promotes lifestyle changes. However, more research is needed to assess the long-term effectiveness of mindfulness for weight control.
TINU syndrome - literature review
Authors: Sowiński Wojciech , Zdybel Michał , Kamińska Magdalena , Strzoda Adam , Strzoda Anna , Strzoda Agata , Rojek Kornelia , Juda Agata
Published: April 2023

Introduction

Tubulointerstitial nephritis with uveitis (TINU syndrome) is a rare condition which mainly affects young females. The pathomechanism of the TINU has not been still well understood but autoimmune background is suspected. Although it was first described in 1975, less than 600 cases have been reported so far.

Aim of the study

The aim of the study is to present the current knowledge about the TINU syndrome.

Material and methods

The article was created based on the PubMed database. Articles were searched in English using the following keywords: TINU syndrome, tubulointerstitial nephritis, uveitis.

Results

TINU syndrome occurs in 0.1-2% of patients with uveitis with no ethnic preferences. It has been linked to a number of potential causes including infectious, genetic, and jatrogenic factors. Clinical manifestation is often atypical what leads to underdiagnosis of disease. There are currently no established specific guidelines on the basis of which a diagnosis of TINU syndrome can be made. Therapy includes topical treatment and systemic corticosteroid therapy. The overall prognosis for patients is favorable, but it is possible to develop chronic kidney disease especially in adults.

Summary:

TINU syndrome remains a poorly understood disease and is often not taken into account in the diagnostic process. The lack of general treatment standards means that therapy is not always effective. Further research is needed to establish guidelines for the management of this disease.
Medical cannabis and neurological disorders: a clinical overview
Authors: Erridge Simon , Sodergren Mikael H
Published: April 2023

In the UK, in addition to licensed medical cannabis products, over 30 000 patients are prescribed unlicensed preparations. The conditions for which these unlicensed products are commonly prescribed and have been identified as having potential therapeutic value, include neurological conditions, as well as those conditions that require modulation of the central and/or peripheral nervous system via targeting the endocannabinoid system, such as during chronic pain. Considering the growing evidence base, this article examines some of the pre-clinical and clinical rationale underpinning the increase in the prescription of these products since their rescheduling in 2018. Beyond this, the article outlines the regulatory environment for prescribing, some of the barriers that patients face, and the work of Sapphire Medical Clinics to uphold stringent clinical governance practices, while providing a patient-centred service.
Complementary and alternative medicine and multiple sclerosis
Authors: Whittam Ali
Published: April 2023

Complementary and alternative medicines (CAMs) are non-mainstream approaches that are used alongside or instead of conventional medicines. As people with multiple sclerosis (MS) are one of the biggest user groups of CAMs, Ali Whittam from the MS Trust, provides a brief overview into what CAMs are and what people with MS, or another neurological condition for that matter, need to consider when sourcing and contemplating the use of CAMs.
The short-term impact of non-removable offloading devices on quality of life in people with recurrent diabetic foot ulcers
Authors: Delpierre Thomas , McCormick Keith , Backhouse Michael R , Bruce Julie , Cherry Lindsey
Published: April 2023

Objective:

Recurrent diabetic foot ulcers (DFUs) are associated with poor health-related quality of life and reduced mobility. Current guidelines recommend application of non-removable offloading devices (NROLDs) as they may improve the healing of DFUs, but there is a lack of information on the wider effects of wearing these devices. Few studies have examined the impact of NROLDs on holistic wellbeing or physical activity. We aimed to investigate the short-term impact of NROLDs on physical activity and DFU-related quality of life (DFU-QoL) in a small sample of community-dwelling people with recurrent DFUs.

Method:

We measured DFU-QoL and physical activity (GPAQ) in people with DFUs, recruited from a single clinic before NROLD application, and at three and six weeks after device fitting. Participants were aged from 39–81 years (mean 58.4±10.1 years) with an equal number of male and female participants.

Results:

The study cohort comprised 18 participants, of whom 14 (78%) completed six-week questionnaires. Although there was some interim decline observed within individual domains of the DFU-QoL (financial: mean difference (MD) 16.2 (95% confidence interval (CI) 2.1, 30.2); p=0.03); non-compliance: MD 12.5 (95% CI–0.2, 25.2); p=0.05), no differences were observed over six weeks. Levels of physical activity declined over time, with over half (56%) of participants classified as having low levels of physical activity at baseline, rising to two-thirds (67%) at follow-up.

Conclusion:

Future studies should explore the longer-term holistic impact of NROLDs and develop more personalised approaches to care at the point of prescription, during and post-device use.
New antioxidant therapy for hard-to-heal neuroischaemic diabetic foot ulcers with deep exposure
Authors: Pérez-Zabala Elena , Basterretxea Andima , Castro Begoña , Aizpuru Alfonso , Arancon Jose Antonio , Moreno Carla , Zubizarreta Arrate , Larizgoitia Zohartze , Ysa August , Lobato Marta , Larrazabal Ainara
Published: April 2023

Objective:

To evaluate the clinical efficacy of a new antioxidant therapy for the treatment of complex neuroischaemic diabetic foot ulcers (DFUs).

Method:

A prospective case series study has been conducted in patients with complex neuroischaemic DFUs after transmetatarsal amputation. DFUs were locally treated with an antioxidant dressing twice a week for the first two weeks, and then once a week until the end of the study or complete wound closure. Patients were followed-up for eight weeks and assessed weekly to analyse wound outcome. Primary outcomes were the wound closure ratio and percentage of granulation tissue; secondary outcomes were parameters related to wound management, namely, presence of non-viable tissue in the wound bed, levels of maceration and exudates, presence of erythema and pain.

Results:

A total of 20 patients were included with a mean baseline wound area of 20.4cm2. At 8 weeks, the mean reduction in wound area was 88.1% (p<0.0001) and complete closure was observed in 33% of cases. In addition, there was a mean increase of 94.7% in granulation tissue in the wound bed (p<0.0001). Furthermore, the therapy was associated with a significant percentage reduction in wounds with non-viable tissue, good exudate management, and the maintenance of low levels of maceration, erythema and pain.

Conclusion:

The new antioxidant therapy was associated with good clinical outcomes in large hard-to-heal neuroischaemic DFUs, with significant wound area reduction and granulation tissue formation. The therapy was also found to be safe and perform well from a practical perspective.
Cold plasma treatment is safe for diabetic foot ulcers and decreases Staphylococcus aureus bacterial load
Authors: Lagrand Rimke S , Sabelis Louise WE , de Groot Vincent , Peters Edgar JG
Published: April 2023

Aim:

Cold atmospheric plasma (CAP) has antimicrobial properties. We studied the safety of a novel CAP device (PLASOMA prototype; Plasmacure, The Netherlands) that is simple to use and could be applied at a patient's home for the treatment of diabetic foot ulcers (DFUs). Secondary objectives were to investigate the effect of CAP on bacterial load and on ulcer size.

Method:

We included subjects with non-infected, superficial DFUs and treated them with CAP on a daily basis for 10 days. The primary endpoint was the occurrence of serious adverse device effects (SADE). We defined safety as: ≤10% of patients experiencing a SADE other than infection (non-infectious SADE), and ≤60% of patients developing infection of the foot (infectious serious adverse event (SAE)).

Results:

We enrolled 20 patients. No SADE occurred, but three infectious SAEs occurred at the site of application within one month of treatment; three SAEs unrelated to treatment occurred, and 55% of subjects reported transient mild adverse device effects. Staphylococcus aureus bacterial load decreased directly after CAP application (p=0.01). The mean decrease of ulcer surface area was 43% (95% confidence interval: 20.2%–65.9%).

Conclusion:

CAP treatment in DFUs was safe and well tolerated. Ulcer size and Staphylococcus aureus colonisation decreased during treatment.
Nurses' knowledge and practice in preventing pressure injuries in intensive care units
Authors: Kaçmaz Hatice Yüceler , Ceyhan Özlem , Güler Hüseyin Burak , Balcılar Fadime
Published: April 2023

Objective:

Globally, pressure injuries (PIs) are an important problem affecting healthcare institutions and patients. Nurses in multidisciplinary teams, play a crucial role in preventing PIs. Nurses working in intensive care units (ICUs) provide care to patients who are at risk of developing PIs. The aim of this study was to ascertain the knowledge and practices of intensive care nurses in preventing PIs, and to highlight the relationship between this knowledge and the prevalence of PIs.

Method:

This study used a descriptive and correlational design. It was carried out in the level 3 ICU of a university hospital in Kayseri, Turkey. A total of four instruments were used at the data collection stage: a nurse information form; the PI prevention knowledge assessment instrument; PI prevention practices; and a PI Prevalence Form.

Results:

The population of the study consisted of 111 nurses, 91 (82%) of whom fully participated. The mean score of the nurses' knowledge in preventing PIs was found to be 43.2±11.4%. The most common answer regarding the clinical use of PI prevention practices was: ‘always applied’. There was no relationship between PI prevalence and PI prevention knowledge scores of the ICU nurses.

Conclusion:

In this study, it was observed that although nurses reported incorporating PI prevention practices in ICUs, their related knowledge level was insufficient.
Use of negative pressure wound therapy on locoregional flaps: a case–control study
Authors: Yuan Kong , Quah Alison , Hwee Jolie , Xu Roland , Yijun Wu , Wen Ng Hui , Han Pek Chong
Published: April 2023

Objective:

The use of negative pressure wound therapy (NPWT) is ubiquitous in the management of complex wounds. Extending beyond the traditional utility of NPWT, it has been used after reconstructive flap surgery in a few case series. The authors sought to investigate the outcomes of NPWT use on flap reconstruction in a case–control study.

Method:

Patients who underwent flap reconstruction between November 2017 and January 2020 were reviewed for inclusion in the study, and divided into an NPWT group and a control group. For patients in the NPWT group, NPWT was used directly over the locoregional flap immediately post-surgery for 4–7 days, before switching to conventional dressings. The control group used conventional dressing materials immediately post-surgery. Outcome measures such as flap necrosis, surgical site infections (SSIs), wound dehiscence as well as time to full functional recovery and hospitalisation duration were evaluated.

Results:

Of the 138 patients who underwent flap reconstruction, 37 who had free flap reconstructions were excluded, and 101 patients were included and divided into two groups: 51 patients in the NPWT group and 50 patients in the control group. Both groups had similar patient demographics, and patient and wound risk factors for impaired wound healing. Results showed that there was no statistically significant difference between flap necrosis, SSIs, wound dehiscence, hospitalisation duration as well as functional recovery rates. Cost analysis showed that the use of NPWT over flaps for the first seven postoperative days may potentially be more cost effective in our setting.

Conclusion:

In this study, the appropriate use of NPWT over flaps was safe and efficacious in the immediate postoperative setting, and was not inferior to the conventional dressings used for reconstructive flap surgery. The main benefits of NPWT over flaps include better exudate management, oedema reduction and potential cost savings. Further studies would be required to ascertain any further benefit.
Do diabetes-related foot ulcer wound fluid measures have clinical utility as biomarkers for healing? A systematic review
Authors: Longfield Matilda SG , Lourdesamy Jacintha , Min Danqing , Twigg Stephen M
Published: April 2023

Objective:

This study aimed to identify potential biomarkers reported in wound fluid of diabetes-related foot ulcers (DRFUs), and their ability to reflect current and prospective wound healing.

Method:

A systematic search was executed following the PRISMA methodology across five chosen databases: MEDLINE, Embase, Scopus, Cochrane Clinical Trials and Cochrane Systematic Reviews. Using keywords and phrases, it yielded 5022 results.

Results:

Based on predetermined inclusion and exclusion criteria, 19 papers were included in the final analysis, among which: seven reported serial temporal biomarker changes in wounds; six reported measures from baseline and related them to healing rate and/or final healing outcome; four papers reported both end-points, and two papers reported solely on baseline biomarker levels in a generalised diabetic foot ulcer group. Across the studies, a total of 46 distinct markers were described from the wound fluid of n=1141 participants. Biomarkers examined included proteases, protease inhibitors, growth factors, chemokines and cytokines, with proteases being the largest subcategory making up 16 (34.8%) of the markers investigated (n=7). Matrix metalloproteinase-9 (MMP-9) was the most frequently investigated protease and it currently holds the most biomarker promise (n=5). Wound bacterial profiles variably related to wound healing outcome (n=5). One study reported biophysical markers rather than biomarkers, including measurement of wound fluid pH. Study quality was generally good. Drawing quantitative comparisons between papers was not possible due to variability in experimental design including sampling and assessment methods.

Conclusion:

These studies collectively indicate several wound fluid measures that could identify DRFU status and outcomes, and that methodological standardisation in the field is needed to determine reliable predictive thresholds for healing.
The Factors Influencing Digital Literacy Practice in Vocational Education: A Structural Equation Modeling Approach
Authors: Jatmoko Dwi , Suyitno Suyitno , Rasul Mohamad Sattar , Nurtanto Muhammad , Kholifah Nur , Masek Alias , Nur Hamid Ramadhan
Published: April 2023

In the future, vocational students will face all changes and developments in technology and information. In this context, students' digital literacy skills need to be trained to adapt to the demands of the world of work. This article aims to present the results of a study on factors that can improve the digital literacy practice for vocational students as a roadmap for digital learning in the current era. The sampling technique used purposive random sampling of 8 Vocational Schools in East Java Province, Indonesia. The number of respondents in this study amounted to 355 second-year students. The study has found that online learning, motivation, and technology introduction are influenced by students' digital literacy practices, while teacher readiness and infrastructure must be mediated by online learning. The findings in this study indicate that collaboration from various parties is needed from teachers, school administrators, and policymakers in planning learning that focuses on students' digital abilities.
The Effect of the Collaborative Discussion Strategy Think-Pair-Share on Developing Students' Skills in solving Engineering Mathematical Problems
Authors: Alsmadi Mohareb. A. , Tabieh Ahmad A.S. , Alsaifi Rula M. , Al-Nawaiseh Sabah Jamil
Published: April 2023

The Think-Pair-Share (TPS) strategy makes the learning environment interactive, lively, collaborative and democratic. It allows students to interact; accept information; develop collaborative discussion skills; refine their thinking; and participate effectively in the classroom. In this study, the researchers investigated the effect of the collaborative discussion strategy (think-pair-share) on developing students' skills in solving engineering mathematical problems. Once we had confirmed the validity and reliability of the tools, we used the quasi-experimental approach. The study sample consisted of 66 students divided into two groups: Namely, an experimental group, which comprised 33 students who studied mathematics using the (think-pair-share) strategy; and a control group, which comprised 33 students who studied in the traditional way. Both groups sat for a pretest and post-test in mathematics. The test results showed that the use of the TPS strategy had a positive effect on developing problem-solving skills compared to the traditional method. In light of these results, the study recommended the use of TPS strategy to improve the skills of students in solving engineering mathematical problems.
Motivating Active Learning in Physical Education: Critical Thinking
Authors: Hidayat Cucu , Lengkana Anggi Setia , Rohyana Aang , Purwanto Didik , Razali Razali , Rosalina Mia
Published: April 2023

Motivation is essential in shaping how much a student is interested in learning and also affects how much students will learn from a learning activity or how much students' ability to capture the information presented by the teacher. Well-motivated students will produce a vibrant learning atmosphere and a better success rate. This research aims to determine whether the motivating active learning in physical education (MALP) model can help kids in elementary school develop their capacity for critical thinking. The design used is experimental. The research subjects were grade 6 elementary school students representing five sub-districts in the Tasikmalaya district. One elementary school was taken from each sub-district through a probability sampling technique using the cluster random sampling approach. The total sample taken was 137 people. The results of the study prove that applying the MALP model can greatly influence improving the critical thinking skills of elementary school students. The result of the study is proven by the significance test using the paired sample t-test; the results obtained from sig. (2-tailed) of .001< .05. So applying motivating active learning in the physical education model influences increasing elementary school students’ critical thinking skills.
Relations Among Psychological Resilience, Exam Anxiety, and School Satisfaction in a Large Sample of Azerbaijani Adolescents
Authors: Rustamov Elnur , Aliyeva Matanat , Nahmatova Ulviyya , Asadov Fuad , Mammadzada Gulay
Published: April 2023

The purpose of this study was to examine whether exam anxiety played a role as a mediator in the relationship between psychological resilience and school satisfaction in a large sample of adolescents (N = 1819; Mage = 15.16, SD = 0.95) who live in different regions of Azerbaijan. Adolescents’ psychological resilience was positively associated with school satisfaction, whereas their exam anxiety level was negatively associated with school satisfaction. Structural equation modeling results showed that exam anxiety partially mediated the relationships between psychological resilience and school satisfaction. Therefore, the indirect effect of psychological resilience on school satisfaction through exam anxiety was also statistically significant.
Products of skew-involutions
Authors: Joven Jesus Paolo , Paras Agnes
Published: April 2023

It is shown that every $2n$-by-$2n$ matrix over a field $\mathbb{F}$ with determinant 1 is a product of (i) four or fewer skew-involutions ($A^2 = -I$) provided $\mathbb{F} \neq \mathbb{Z}_3$, and (ii) eight or fewer skew-involutions if $\mathbb{F} = \mathbb{Z}_3$ and $n > 1$. Every real symplectic matrix is a product of six real symplectic skew-involutions, and an explicit factorization of a complex symplectic matrix into two symplectic skew-involutions is given.
Characteristics of Maleo bird spawning nests (Macrocephalon maleo) in Lake Towuti, South Sulawesi, Indonesia
Authors: Karim Hadijah Azis , Najib Nardy Noerman , Ayu Srida Mitra , Fidel Fidel
Published: April 2023

Abstract. Karim HA, Najib NN, Ayu SM, Fidel. 2023. Characteristics of Maleo bird spawning nests (Macrocephalon maleo) in Lake Towuti, South Sulawesi.Biodiversitas 24: 670-676. Maleo birds (Macrocephalon maleo) have a habitat in the forest near the beach or in the middle of the forest near the hot springs. However, in Pekaloa Village, East Luwu District, Maleo birds are found in Lake Towuti, South Sulawesi, Indonesia. This, of course, shows that the habitat of Maleo birds on the shore of the lake is new information, especially on the island of Sulawesi. This research was conducted in Pekaloa Village, Towuti Sub-district, East Luwu District, South Sulawesi. This study was conducted to collect data on the characteristics of Maleo Senkawor (Macrocephalon maleo) nests. Secondary data collection began from scientific journals and works, as well as discussions with the surrounding community and the TWA manager of Lake Towuti, while primary data was obtained from measurements and direct observation in the field, as well as laboratory analysis. In this study, five nest plots with 13 nest holes were used, and the vegetation analysis used example plots measuring 175 m2. The characteristics of the nest pit have an average depth of 0.6 m, a surface diameter of 0.41 m, a temperature of 33.6oC, and a pH of 6.2. Vegetation analysis found eight species around the Maleo Senkawor bird's nest. Vegetation analysis used the density formula: highest frequency density 19.5 and highest frequency 0.57, lowest density 0.02 and lowest frequency 0.14. This study forms the basis of new information in the conservation of Maleo birds with the characteristics of the habitat conditions for nesting Maleo birds, especially in the coastal area of the lake.
Phylogenetic of red snapper (Lutjanidae) in Yapen Island Waters, Papua, Indonesia
Authors: Sala Ridwan , Kusuma Aradea Bujana , Pranata Bayu
Published: April 2023

Abstract. Sala R, Kusuma AB, Pranata B. 2022. Phylogenetic of red snapper (Lutjanidae) in Yapen Island Waters, Papua, Indonesia. Biodiversitas 23: 6428-6436. Red snappers are an economically valuable fishery resource. Most of these snapper species are inhabitants of coral reef ecosystems. A variety of red snapper species can be found in the northern waters of Papua, particularly in the Yapen Regency. However, information regarding the biological and ecological aspects of these fish is virtually unavailable. On the other hand, the utilization intensity of red snapper as a source of community income continues to increase. By using DNA barcode sequences, this study attempted to examine species diversity and relationships among Lutjanidae family species. The study was carried out from June to August 2022 in the waters of Yapen Regency by taking fish samples from the fish caught by local hand-lining fishers. Red snapper samples were identified morphologically and molecularly. According to molecular analyses, it was identified nine red snapper species from the genera of Lutjanus, Aphareus and Pristipomoides. The morphological characteristics of the species from the genus of Aphareus are similar to those from the genus of Pristipomoides yet different from those of the species from the genus of Lutjanus. The phylogenetic tree consisted of four clades with significant bootstrap values ranging from 98 to 99%. Clades 1, 2 and 3 comprise the species from the genus of Lutjanus, while clade 4 contains species from the genera of Aphareus and Pristipomoides. The greatest genetic distance was found between Lutjanus fulvus and Pristipomoides multidens, while the smallest genetic distance was found between Lutjanus vitta and Lutjanus ehrenbergii. Based on the study results, some management implications are discussed. For example, information on species biodiversity maps is needed to determine the target stock of a managed species so that management objectives are more focused on the species level and not on the genus or family level.
Sex-linked Single Nucleotide Polymorphism (SNP) identification and molecular marker development of salacca (Salacca zalacca (Gaertn.) Voss)
Authors: PRIHATINI RIRY , DINARTI DINY , SUTANTO AGUS , SUDARSONO SUDARSONO
Published: April 2023

Abstract. Prihatini R, Dinarti D, Sutanto A, Sudarsono. 2023. Sex-linked Single Nucleotide Polymorphism (SNP) identification and molecular marker development of salacca (Salacca zalacca (Gaertn.) Voss). Biodiversitas 24: 704-712. Salacca zalacca (Gaertn.) Voss is a native tropical fruit species of Indonesia with three different sex types, i.e., female, male, and hermaphrodite. Early sex identification plays a vital role in the dioecious plant development, cultivation, and breeding program. The morphological and histological markers cannot differentiate salacca sex types during vegetative stages, during which molecular approaches are offered. Isolation of salacca's partial Transducin Beta Like-3 (TBL3) gene was done using Phoenix dactylifera L. TBL3-specific primers. After DNA sequencing and multiple sequence alignment, the putative TBL3 aligned data was used to construct a phylogenetic tree, find SNAP primers, and use the generated markers as predictors for sex-type phenotypes of salacca. Phylogenetic analysis showed that the dendrogram of aligned TBL3 partial sequences of salacca and other taxa showed that the salacca TBL3 is closely related to P. dactylifera and Elaeis guineensis Jacq. since the three palms belong to the same Arecaceae family. The partial sequence of the TBL3 gene of hermaphrodite salacca revealed ten single nucleotide polymorphisms (SNPs) compared to those of female and male plants. Protein alignment of the sequence also showed several differences, which may lead to different flower morphogenesis. The salacca SNAP sex-types markers were developed from one of the SNP sites and validated using samples from farmer plantations. The SNAP primer pairs of STBL1F-Ref - STBL1-Rev amplified a 300 bp fragment from hermaphrodite samples, whereas no band was amplified from the female and male samples. On the contrary, primers STBL1F-Alt - STBL1-Rev amplified a 300 bp fragment from female and male plants but not from the hermaphrodite plants. The salacca's TBL3 sequences and the SNAP markers are valuable tools for further salacca genomic exploration.
Determination of Begomovirus on chili plants (Capsicum sp.) in Buton and Muna Islands, Southeast Sulawesi, Indonesia
Authors: WIDODO CATUR JOKO , TAUFIK MUHAMMAD , KHAERUNI ANDI , MALLARANGENG RAHAYU
Published: April 2023

Abstract. Widodo CJ, Taufik M, Khaeruni A, Mallaranggeng R. 2023. Determination of Begomovirus on chili plants (Capsicum sp.) in Buton and Muna Islands, Southeast Sulawesi, Indonesia. Biodiversitas 24: 741-751. Begomovirus disease is one of the main factors inhibiting chili cultivation which could harm plants and result in crop failure. This study aimed to determine the symptoms of begomovirus disease in chili plants in Buton and Muna, Southeast Sulawesi. The methods used are disease incidence survey, insect vector observation, molecular identification using the polymerase chain reaction (PCR) technique, and Sanger sequencing (Applied Biosystem 3500). The survey was conducted in Southeast Sulawesi province, covering Bau-Bau City, Buton Regency, Central Buton, North Buton, Muna, and West Muna. The results showed that the incidence and severity of Begomovirus disease were observed highest in Buton islands, 73% and 49.5% (Buton City), followed by North Buton, Central Buton, and Buton, with the incidence and severity of Begomovirus, respectively, of 71.74% and 59.5%; 69.32% and 51.5%; and 68% and 61.5%. While in Muna Regency, 63.16% and 38.5%, West Muna, 73.79% and 53.50%. On the other hand, the highest percentage of the whitefly population was in Bau-Bau City, 24%, while the lowest was in West Muna, 15.5% supporting this observation. Furthermore, PCR analysis successfully amplified symptomatic isolates from Buton and Muna islands at a band length of 580 bp. On the other hand, Sanger sequencing analysis revealed the existence of the Indonesian Pepper Yellow Leaf Curl Virus (PepYLCIV) with more than 96% genetic homology to the Gene Bank data of MN094866 PepYLCIV Capsicum annuum Indonesia and MN738463 PepYLCIV Chili pepper Indonesian. This information is the first report of the presence of Begomovirus on the two islands.
Short Communication: Characteristics of the endangered Javan banteng (Bos javanicus) spermatozoa
Authors: Yoelinda Vincentia Trisna , Arifiantini Raden Iis , Solihin Dedy Duryadi , Agil Muhammad , Setiadi Dedi Rahmat , Hastuti Yohana Tri , Manansang Jansen , Sajuthi Dondin
Published: April 2023

Abstract. Yoelinda VT, Arifiantini RI, Solihin DD, Agil M, Setiadi DR, Hastuti YT, Manansang J, Sajuthi D. 2023. Short Communication: Characteristics of the endangered Javan banteng (Bos javanicus) spermatozoa. Biodiversitas 24: 759-766. Morphology and morphometry of spermatozoa become essential fertility parameters in domestic and wild animals. Such morphological abnormalities can arise from genetic or environmental origins and cause fertility deprivation. Semen collection using transrectal massage has been used in several domestic and wild animals and is known to be simpler, safer, and less painful than artificial vaginas and electro-ejaculation. This study aimed to evaluate morphological and morphometrical features of the banteng spermatozoa head. The objective of this study was also, to evaluate the use of transrectal massage as a semen collection protocol. This study was conducted in Taman Safari Indonesia, Cisarua, Bogor, West Java, involving one banteng (Bos javanicus) bull. Semen samples were collected weekly, smeared, and stained with a modified Williams' staining method. This study found ten types of head morphological abnormality, comprising several major defects, with several types known to be the genetic origin. There were also nine types of spermatozoa tail abnormalities and teratoid form abnormalities. The total normal spermatozoa proportion in this study was 54.30±1.74%. Morphometric dimensions of length, width, and area head of banteng spermatozoa were 9.88±0.01 µm, 4.91 µm, and 38.12±0.05 µm2, respectively, with a tail length of 60.48±0.03 ?m. Although our result was limited by the number of samples available for the study, this first report on the banteng bull's sperm morphology and morphometry still need to be considered. The possibility of transrectal massage use for banteng semen collection protocol demonstrated in this study may be helpful to support the conservation approach in terms of breeding program strategy.
Flowering and fruiting phenology of Anaxagorea luzonensis A. Gray (Annonaceae)
Authors: LESTARI DEWI AYU , NINGRUM LINDA WIGE , NADA MAFTUKHAKH HILMYA , PRADIPTA NARESVARA NIRCELA , HARSONO DIAN RAKHMAWATI
Published: April 2023

Abstract. Lestari DA, Ningrum LW, Nada FMH, Pradipta NN, Harsono DR. 2023. Flowering and fruiting phenology of Anaxagorea luzonensis A. Gray (Annonaceae). Biodiversitas 24: 784-792. Anaxagorea luzonensis is one of the critical plant collections from the Annonaceae family, with only one specimen in Purwodadi Botanic Garden (PBG). In protecting these plants, it is necessary to know the process of flowering and fruiting through the phenological process. This research is expected to observe plant propagation, physiology, environmental response, and increased reproductive success. The aim of this research is to know the details of flowering and fruiting phenology stages, determine reproductive success, and explain the floral biology of A. luzonensis. Research on the flowering and fruiting phenology of A. luzonensis was carried out from December 2021 – November 2022 in one season at PBG. The method is observative, including changes in color, shape, and size of flowers and fruit in each phenological stage. In addition, the method includes the count of reproductive success and observation of the A. luzonensis flower. This study's results stated that the flowering and fruiting phenology of A. luzonensis experienced a shorter flowering time than fruiting time and a low percentage (<30%) of reproductive success. Flowering stages are divided into five stages, i.e. initiation, flower bud, before bloom, bloom, and anthesis; fruiting stages are divided into three stages, i.e. immature-sized, mature-sized, and ripe fruit, which occurred in period 73-121 ± 85 days. Environmental and genetic factors cause the low value of reproductive success. This research can be useful for the conservation strategy of A. luzonensis, especially for reproduction biology and seed conservation.
The influence of inoculum types on the chemical characteristics and b-glucan content of tempe gembus
Authors: RIZAL SAMSUL , KUSTYAWATI MARIA ERNA , MURHADI MURHADI , AMIN MUHAMAMAD
Published: April 2023

Abstract. Rizal S, Kustyawati ME, Murhadi, Amin M. 2023. The influence of inoculum types on the chemical characteristics and b-glucan content of tempe gembus. Biodiversitas 24: 793-798. Tempe gembus is a type of tempe which is made by fermenting tofu dregs with Rhizopus oligosporus. Tempe gembus has lower nutritional value than soybean tempe. Adding Saccharomyces cerevisiae to manufacture tempe gembus is expected to increase the added value of tempe gembus. The aim of this study was to determine the effect of various types of inoculums on the chemical properties and b-glucan content of tempe gembus. The study used a completely randomized block design (RBD) with 3 repetitions and 7 types of inoculums: commercial tempe inoculum (RAPRIMA) (T1), S. cerevisiae (T2), R. oligosporus (T3), commercial tempe inoculum (RAPRIMA) + commercial yeast (Fermipan) (T4), commercial tempe inoculum (RAPRIMA) + S. cerevisiae (T5), R. oligosporus + Fermipan (T6), and R. oligosporus + S. cerevisiae (T7). Tempe gembus produced was analyzed for the content of fat, protein, ash, water, carbohydrates, and b-glucan. The data obtained was analyzed statistically using one way ANOVA and the Honest Significant Difference (HSD) test. The results showed that the type of tempe inoculum increased the levels of protein, ash, water and b-glucan but decreased the levels of fat and carbohydrates in tempe gembus. A mixture of R. oligosporus and S. cerevisiae produced the best tempe gembus containing 0.69% ?-glucan, 6.98% protein, 0.48% ash, 83.98% water, 8.12% carbohydrates and 0.47% fat.
Agronomic performance and selection of green super rice doubled haploid lines from anther culture
Authors: NURHIDAYAH SITI , PURWOKO BAMBANG SAPTA , DEWI ISWARI SARASWATI , SUWARNO WILLY BAYUARDI , LUBIS ISKANDAR
Published: April 2023

Abstract. Nurhidayah S, Purwoko BS, Dewi IS, Suwarno WB, Lubis I. 2023. Agronomic performance and selection of green super rice doubled haploid lines from anther culture. Biodiversitas 24: 819-826. Rice is the staple food of most Asian, including Indonesian people. Plant breeding plays an important role in producing high-yielding varieties of rice to meet national carbohydrate sufficiency. Green Super Rice (GSR) is an alternative for rice variety development that has the advantages of high yield, good quality, pest resistance, and efficient fertilizer use to support sustainable agriculture. This study aimed to evaluate the agronomic character of GSR doubled haploid (DH) lines derived from anther culture and determine the best GSR rice lines based on index selection. The treatment was 65 genotypes consisting of 60 DH of GSR lines from anther culture of 5 combinations of crosses and 5 check varieties. The study used a randomized complete block design with three replications. The results showed that most of the tested lines had similar agronomic performances to the check variety Inpari 42 Agritan GSR. Almost all agronomic characters of the GSR DH lines had high genetic variability. Most characters had a high category of broad-sense heritability except panicle length. Line selection using index selection for economically important agronomic characters yielded 27 GSR DH lines having high productivity, number of productive tillers per hill, number of total grains per panicle, and percentage of filled grains per panicle.
Mapping coral cover using Sentinel-2A in Karimunjawa, Indonesia
Authors: SATYA ERIDHANI DHARMA , SABDONO AGUS , WIJAYANTI DIAH PERMATA , HELMI MUHAMMAD , WIDIARATIH RIKHA , SURYOPUTRA AGUS ANUGROHO DWI , HANDOYO GENTUR , PURYAJATI ARDIANSYAH DESMONT
Published: April 2023

Abstract. Satya ED, Sabdono A, Wijayanti DP, Helmi M, Widiaratih R. 2023. Mapping coral cover using Sentinel-2A in Karimunjawa, Indonesia. Biodiversitas 24: 827-836. Coral reefs in the Karimunjawa National Park (KNP) have their own charm, especially for snorkeling and divers, it can be seen from the number of tourist visits which tends to increase every year, except for 2020 to 2021 due to the COVID-19 pandemic. This study aims to determine the impact of tourism on coral reef cover in KNP, with islands selected as representatives of high, medium and low level of tourist visits using Sentinel-2A. Comparison of Sentinel-2A coral cover area in 2020 with previous research which used Landsat 8 data in 2018 showed decrease in coral cover area by 16.5% and an increase in sand area by 24.1% for island of high tourist visits. It can be seen that tourism activities have significant impact on coral cover area, especially snorkeling and diving activities that are intentional or not potentially damaging to coral reefs. Sentinel-2A's ability to detect coral area is reliable, since field data is needed as input for data processing to identification locations in detail as needed. Coral area in 2020 using Sentinel-2A obtained for Menjangan Besar Island and Menjangan Kecil, Sintok Island, Tengah Island and Seruni Island were 1.88 km2, 0.303 km2, 0.318 km2, 0.237 km2 respectively.
Subtrochanteric Pathological Femur Fracture in an HIV-Positive Patient on Tenofovir-based antiretroviral therapy: A Case Report and Review of the Literature
Authors: Choudhary Siddharth , Rangasamy Karthick , Naidu Shankar , Sachdeva Ravinder Kaur , Sharma Aman
Published: April 2023

Introduction: The population of people living with human immunodeficiency virus (HIV) and AIDS has increased and so is the incidence of fragility fractures in these patients. Multiple contributory factors are responsible for osteomalacia or osteoporosis in such patients such as a chronic inflammatory response to the HIV, highly active antiretroviral therapy (HAART) itself, and associated comorbidities. Tenofovir has also been reported to disrupt bone metabolism and causes fragility fractures. Case Report: A 40-year-old HIV-positive female came to us with pain in her left hip and was unable to bear weight. She had a history of trivial fall. The patient has been taking tenofovir-associated HAART regimen for the past 6 years and has been compliant. She was diagnosed with a left-side transverse subtrochanteric closed femur fracture. Closed reduction and internal fixation was done using a proximal femur intramedullary nail (PFNA). The latest follow-up shows fracture union and good functional outcomes after treating osteomalacia, and HAART changed to a non-tenofovir regimen later. Conclusion: Patients with HIV infection are prone to fragility fractures and periodic monitoring of their BMD, serum calcium, and vitamin D3 levels should be done for prevention and early diagnosis. More vigilance in patients receiving a tenofovir-associated HAART regimen is needed. Appropriate medical treatment needs to be started once any abnormality in the bone metabolic parameters is detected, and drugs like tenofovir need to be changed as it causes osteomalacia. Keywords: Human immunodeficiency virus, fracture, pathological, HAART, tenofovir, osteomalacia.