bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Drought on Yield and Nutraceutical Properties of Beans (Phaseolus spp.) Traditionally Cultivated in Veneto, Italy
Authors: Sica Pietro , Galvao Aline , Scariolo Francesco , Maucieri Carmelo , Nicoletto Carlo , Pilon Cristiane , Sambo Paolo , Barcaccia Gianni , Borin Maurizio , Cabrera Miguel , Franklin Dorcas
Published: January 2021

Beans are often grown in regions with climates that are susceptible to drought during the cultivation period. Consequently, it is important to identify bean accessions tolerant to drought conditions and assess the effect of drought on seeds’ nutraceutical properties. This study evaluated the effect of drought during different development stages (NES = never stressed; ALS = always stressed; SBF = stressed before flowering; SAF = stressed after flowering) on the yield and nutraceutical properties of six local bean varieties: Fasolo del Diavolo, Gialet, Posenati, Secle, D’oro, and Maron. Analysis of variance indicated that Gialet was not significantly affected by drought treatments, and Posenati under SBF and NES treatments had greater yields than under ALS and SAF treatments, whereas Secle under SBF produced 80% more seeds than under NES. Total phenols, antioxidant capacity, and calcium content were significantly different among the local varieties. Yield was significantly and positively correlated with seed calcium content and significantly and negatively correlated with protein, total phenols, and antioxidant capacity. The interaction between local varieties and treatment significantly affected seeds’ Zn content. Gialet and Maron seeds’ Zn contents were about 60 mg kg−1, almost double the average of commercial varieties. In summary, this study paves the way to the identification of potential bean varieties resistant to drought. Further molecular studies will help support these findings.
Accuracy of Community Informant Led Detection of Maternal Depression in Rural Pakistan
Authors: Mohsin Shamaila , Waqas Ahmed , Atif Najia , Rabbani Muhamamd Waqas , Ali Khan Shahzad , Bilal Samina , Sharif Maria , Bibi Amina , Sikander Siham
Published: January 2021

Maternal depression is a global mental health and a public health priority. Despite the priority its active detection is still a challenge. We tested the accuracy of an adapted version of Community Informant Detection Tool for Maternal Depression (CIDT-MD) in rural settings of Pakistan. Using a single stage design, trained community informants (lady health workers and lay peers) identified women (pregnant and/or with children) with symptoms of probable depression using CIDT-MD. This was immediately followed by diagnostic interviews of all the women using the Structured Clinical Interview for the Diagnostic and Statistical Manual (SCID-V) for current major depressive episode by trained assessors, blinded to the outcome of CIDT-MD. Data were analyzed using Statistical Package for Social Sciences (Version 25.0, IBM Corp., Armonk, NY, USA) and FACTOR software (Version. 10.3.01, Virgili University, Tarragona, Spain). Descriptive statistics, factor analysis, validity, reliability and known group validity was conducted to evaluate the psychometric properties of the adapted CIDT-MD. In all, 425 women, with mean age of 28 years (SD = 4.7), participated. Nearly 10% were illiterate, while the rest (90%) had an education ranging from eight to 15 years of schooling. The majority (73.2%) of the participants had 1–3 children while only 17.4% had >3 children. The sensitivity and specificity of CIDT-MD in detecting depressive symptoms was 97.5% (95% CI: 94.2–99.1) and 82.4% (95% CI: 77.8–86.4) respectively. It’s positive predictive value (PPV), 77.3% (95% CI: 72.9–81.2) and the negative predictive value (NPV) was 98.17% (95% CI: 95.7–99.2). While factor analysis revealed high inter-item correlation for most items (0.62–0.77) with an adequately fair Kaiser-Meyer-Olkin (KMO) sampling adequacy (0.73), significant Bartlett’s test of sphericity (p < 0.001). Uni-dimensionality for the CIDT-MD based on one-dimensional congruence (0.97), explained common variance (0.85), excellent internal consistency (0.90), good criterion validity (Area Under Curve = 81%), tester-test reliability (0.87–0.89) and statistically significant known group analysis (p < 0.001). The adapted version of the Community Informant Detection Tool for Maternal Depression is a valid and a reliable tool for active case detection of maternal depression in rural settings of Pakistan.
ArcStereoNet: A New ArcGIS® Toolbox for Projection and Analysis of Meso- and Micro-Structural Data
Authors: Ortolano Gaetano , D’Agostino Alberto , Pagano Mario , Visalli Roberto , Zucali Michele , Fazio Eugenio , Alsop Ian , Cirrincione Rosolino
Published: January 2021

ArcStereoNet is a new ArcGIS® based toolbox for stereographic projections that we implement here using Python 2.7 programming language. The reason to develop another stereographic projection package arises from the recent use of Python as an exclusive programming language within the ArcGIS® environment. This permits a more flexible approach for the development of tools with very intuitive GUIs, and also allows the user to take full advantage of all potential GIS mapping processes. The core of this new projections toolbox is based on the capability to easily apply and compare most of the commonly used statistical methods for cluster and girdle analysis of structural data. In addition to the well-known Fisher, K-means, and Bingham data elaborations, a completely new algorithm for cluster analysis and mean vector extraction (Mean Extractor from Azimuthal Data), was developed, thereby allowing a more reliable interpretation of any possible structural data distribution. Furthermore, as in any other GIS platform, users can always precisely correlate each single projected data point with the corresponding geographical/locality position, thereby merging or subdividing groups of structural stations with a simple selection procedure. ArcStereoNet also creates rose diagrams, which may be applied not only to fault/joint planes orientation data, but also for the analysis of 2D microstructural fabric parameters. These include geometrical datasets derived from the minimum bounding approach as applied to vectorized grains in thin sections. Finally, several customization settings ensure high-quality graphic outputs of plots, that also allow easy vector graphics post-processing.
Loss of Cnot6l Impairs Inosine RNA Modifications in Mouse Oocytes
Authors: Brachova Pavla , Alvarez Nehemiah S. , Christenson Lane K.
Published: January 2021

Mammalian oocytes must degrade maternal transcripts through a process called translational mRNA decay, in which maternal mRNA undergoes translational activation, followed by deadenylation and mRNA decay. Once a transcript is translationally activated, it becomes deadenylated by the CCR4-NOT complex. Knockout of CCR4-NOT Transcription Complex Subunit 6 Like (Cnot6l), a deadenylase within the CCR4-NOT complex, results in mRNA decay defects during metaphase I (MI) entry. Knockout of B-cell translocation gene-4 (Btg4), an adaptor protein of the CCR4-NOT complex, results in mRNA decay defects following fertilization. Therefore, mechanisms controlling mRNA turnover have significant impacts on oocyte competence and early embryonic development. Post-transcriptional inosine RNA modifications can impact mRNA stability, possibly through a translation mechanism. Here, we assessed inosine RNA modifications in oocytes, eggs, and embryos from Cnot6l-/- and Btg4-/- mice, which display stabilization of mRNA and over-translation of the stabilized transcripts. If inosine modifications have a role in modulating RNA stability, we hypothesize that in these mutant backgrounds, we would observe changes or a disruption in inosine mRNA modifications. To test this, we used a computational approach to identify inosine RNA modifications in total and polysomal RNA-seq data during meiotic maturation (GV, MI, and MII stages). We observed pronounced depletion of inosine mRNA modifications in samples from Cnot6l-/-, but not in Btg4-/- mice. Additionally, analysis of ribosome-associated RNA revealed clearance of inosine modified mRNA. These observations suggest a novel mechanism of mRNA clearance during oocyte maturation, in which inosine-containing transcripts decay in an independent, but parallel mechanism to CCR4-NOT deadenylation.
The Phylogenetic Structure of Reptile, Avian and Uropathogenic Escherichia coli with Particular Reference to Extraintestinal Pathotypes
Authors: Książczyk Marta , Dudek Bartłomiej , Kuczkowski Maciej , O’Hara Robert , Korzekwa Kamila , Wzorek Anna , Korzeniowska-Kowal Agnieszka , Upton Mathew , Junka Adam , Wieliczko Alina , Ratajszczak Radosław , Bugla-Płoskońska Gabriela
Published: January 2021

The impact of the Gram-negative bacterium Escherichia coli (E. coli) on the microbiomic and pathogenic phenomena occurring in humans and other warm-blooded animals is relatively well-recognized. At the same time, there are scant data concerning the role of E. coli strains in the health and disease of cold-blooded animals. It is presently known that reptiles are common asymptomatic carriers of another human pathogen, Salmonella, which, when transferred to humans, may cause a disease referred to as reptile-associated salmonellosis (RAS). We therefore hypothesized that reptiles may also be carriers of specific E. coli strains (reptilian Escherichia coli, RepEC) which may differ in their genetic composition from the human uropathogenic strain (UPEC) and avian pathogenic E. coli (APEC). Therefore, we isolated RepECs (n = 24) from reptile feces and compared isolated strains’ pathogenic potentials and phylogenic relations with the aforementioned UPEC (n = 24) and APEC (n = 24) strains. To this end, we conducted an array of molecular analyses, including determination of the phylogenetic groups of E. coli, virulence genotyping, Pulsed-Field Gel Electrophoresis-Restriction Analysis (RA-PFGE) and genetic population structure analysis using Multi-Locus Sequence Typing (MLST). The majority of the tested RepEC strains belonged to nonpathogenic phylogroups, with an important exception of one strain, which belonged to the pathogenic group B2, typical of extraintestinal pathogenic E. coli. This strain was part of the globally disseminated ST131 lineage. Unlike RepEC strains and in line with previous studies, a high percentage of UPEC strains belonged to the phylogroup B2, and the percentage distribution of phylogroups among the tested APEC strains was relatively homogenous, with most coming from the following nonpathogenic groups: C, A and B1. The RA-PFGE displayed a high genetic diversity among all the tested E. coli groups. In the case of RepEC strains, the frequency of occurrence of virulence genes (VGs) was lower than in the UPEC and APEC strains. The presented study is one of the first attempting to compare the phylogenetic structures of E. coli populations isolated from three groups of vertebrates: reptiles, birds and mammals (humans).
KMU-1170, a Novel Multi-Protein Kinase Inhibitor, Suppresses Inflammatory Signal Transduction in THP-1 Cells and Human Osteoarthritic Fibroblast-Like Synoviocytes by Suppressing Activation of NF-κB and NLRP3 Inflammasome Signaling Pathway
Authors: Baek Hye Suk , Hong Victor Sukbong , Kim Sang Hyon , Lee Jinho , Kim Shin
Published: January 2021

Protein kinases regulate protein phosphorylation, which are involved in fundamental cellular processes such as inflammatory response. In this study, we discovered a novel multi-protein kinase inhibitor, KMU-1170, a derivative of indolin-2-one, and investigated the mechanisms of its inflammation-inhibiting signaling in both THP-1 cells and human osteoarthritic fibroblast-like synoviocytes (FLS). We demonstrated that in THP-1 cells, KMU-1170 inhibited lipopolysaccharide (LPS)-induced upregulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and, furthermore, suppressed LPS-induced phosphorylation of transforming growth factor-β-activated kinase 1, JNK, ERK, inhibitor of NF-κB kinase α/β (IKKα/β), and NF-κB p65 as well as nuclear translocation of NF-κB p65. Moreover, KMU-1170 suppressed LPS-induced upregulation of proinflammatory cytokines such as IL-1β, TNF-α, and IL-6, and, notably, inhibited LPS-induced upregulation of the NLRP3 inflammasome in THP-1 cells. Importantly, KMU-1170 attenuated LPS-mediated inflammatory responses in human osteoarthritic FLS, such as the upregulation of IL-1β, TNF-α, IL-6, iNOS, and COX-2 and the phosphorylation of IKKα/β and NF-κB p65. Collectively, these results suggest that KMU-1170 inhibits inflammatory signal transduction and could be developed as a potential anti-inflammatory agent.
Purinergic Regulation of Endothelial Barrier Function
Authors: Aslam Muhammad , Gündüz Dursun , Troidl Christian , Heger Jacqueline , Hamm Christian W. , Schulz Rainer
Published: January 2021

Increased vascular permeability is a hallmark of several cardiovascular anomalies, including ischaemia/reperfusion injury and inflammation. During both ischaemia/reperfusion and inflammation, massive amounts of various nucleotides, particularly adenosine 5′-triphosphate (ATP) and adenosine, are released that can induce a plethora of signalling pathways via activation of several purinergic receptors and may affect endothelial barrier properties. The nature of the effects on endothelial barrier function may depend on the prevalence and type of purinergic receptors activated in a particular tissue. In this review, we discuss the influence of the activation of various purinergic receptors and downstream signalling pathways on vascular permeability during pathological conditions.
Mediterranean Diet for the Prevention of Gestational Diabetes in the Covid-19 Era: Implications of Il-6 In Diabesity
Authors: Fedullo Anna Lucia , Schiattarella Antonio , Morlando Maddalena , Raguzzini Anna , Toti Elisabetta , De Franciscis Pasquale , Peluso Ilaria
Published: January 2021

The aim of this review is to highlight the influence of the Mediterranean Diet (MedDiet) on Gestational Diabetes Mellitus (GDM) and Gestational Weight Gain (GWG) during the COVID-19 pandemic era and the specific role of interleukin (IL)-6 in diabesity. It is known that diabetes, high body mass index, high glycated hemoglobin and raised serum IL-6 levels are predictive of poor outcomes in coronavirus disease 2019 (COVID-19). The immunopathological mechanisms of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection include rising levels of several cytokines and in particular IL-6. The latter is associated with hyperglycemia and insulin resistance and could be useful for predicting the development of GDM. Rich in omega-3 polyunsaturated fatty acids, vitamins, and minerals, MedDiet improves the immune system and could modulate IL-6, C reactive protein and Nuclear Factor (NF)-κB. Moreover, polyphenols could modulate microbiota composition, inhibit the NF-κB pathway, lower IL-6, and upregulate antioxidant enzymes. Finally, adhering to the MedDiet prior to and during pregnancy could have a protective effect, reducing GWG and the risk of GDM, as well as improving the immune response to viral infections such as COVID-19.
Comparison of the Use of Arterial Doppler Waveform Classifications in Clinical Routine to Describe Lower Limb Flow
Authors: Guilcher Antoine , Lanéelle Damien , Hoffmann Clément , Guillaumat Jérôme , Constans Joel , Bressollette Luc , Le Hello Claire , Boissier Christian , Bura-Rivière Alessandra , Jaquinandi Vincent , Omarjee Loukman , Lacroix Philippe , Pernod Gilles , Abbadie Fabrice , Sevestre Marie Antoinette , Boulon Carine , Mahé Guillaume
Published: January 2021

Background: Characterisation of arterial Doppler waveforms is a persistent problem and a source of confusion in clinical practice. Classifications have been proposed to address the problem but their efficacy in clinical practice is unknown. The aim of the present study was to compare the efficacy of the categorisation rate of Descotes and Cathignol, Spronk et al. and the simplified Saint-Bonnet classifications. Methods: This is a multicentre prospective study where 130 patients attending a vascular arterial ultrasound were enrolled and Doppler waveform acquisition was performed at the common femoral, the popliteal, and the distal arteries at both sides. Experienced vascular specialists categorized these waveforms according to the three classifications. Results: of 1033 Doppler waveforms, 793 (76.8%), 943 (91.3%) and 1014 (98.2%) waveforms could be categorized using Descotes and Cathignol, Spronk et al. and the simplified Saint-Bonnet classifications, respectively. Differences in categorisation between classifications were significant (Chi squared test, p < 0.0001). Of 19 waveforms uncategorized using the simplified Saint-Bonnet classification, 58% and 84% were not categorized using the Spronk et al. and Descotes and Cathignol classifications, respectively. Conclusions: The results of the present study suggest that the simplified Saint-Bonnet classification provides a superior categorisation rate when compared with Spronk et al. and Descotes and Cathignol classifications.
The Effects of Association of Topical Polydatin Improves the Preemptive Systemic Treatment on EGFR Inhibitors Cutaneous Adverse Reactions
Authors: Bavetta Mauro , Silvaggio Dionisio , Campione Elena , Sollena Pietro , Formica Vincenzo , Coletta Deborah , Graziani Grazia , Romano Maria Concetta Pucci , Roselli Mario , Peris Ketty , Bianchi Luca
Published: January 2021

Epidermal Growth Factor Receptor inhibitors (EGFRi) are approved as therapeutic options in several solid tumors. Cutaneous papulopustular eruption is the most frequent cutaneous adverse-event (AE), usually treated with emollient or corticosteroids according to toxicity grade. Our study evaluated the efficacy and safety of a topical product containing polydatin, a glycosylated polyphenol, natural precursor of resveratrol showing anti-inflammatory and anti-oxidative activities, for the prevention and treatment of skin papulopustular rash in EGFRi-treated patients. Forty oncologic patients treated with EGFRi were enrolled in two groups: group-A, 20 patients with papulopustular AE, and group-B, 20 patients without cutaneous manifestations. The study consisted of twice-daily application of polydatin cream 1.5% (group-A) and 0.8% (group-B) for 6 months. In group-A patients, we observed at week 4 a remarkable improvement of skin manifestation and quality of life evaluated with National-Cancer-Institute-Common-Terminology-Criteria for Adverse-Events (NCI-CTCAE), Dermatology-Life-Quality-Index (DLQI) score and Visual-Analogue-Scale (VAS) pruritus, with a statistical significance of p < 0.05. None of the patients of group-B developed skin AEs to EGFRi. No cutaneous AEs related to the polydatin product were reported in both groups. Polydatin can be a good topical aid for the prevention and management of papulopustular rash in cancer patients receiving EGFRi, also capable of improving cancer patients’ quality of life.
Poly(methyl methacrylate) Bone Cement Composite Can Be Refilled with Antibiotics after Implantation in Femur or Soft Tissue
Authors: Cyphert Erika L. , Zhang Ningjing , Marques Dylan W. , Learn Greg D. , Zhang Fang , von Recum Horst A.
Published: January 2021

While periprosthetic joint infections (PJIs) result in a small percentage of patients following arthroplasties, they are challenging to treat if they spread into bone and soft tissue. Treatment involves delivering antibiotics using poly(methyl methacrylate) (PMMA) bone cement. However, antibiotic release is insufficient for prolonged infections. Previous work demonstrated efficacy of incorporating insoluble cyclodextrin (CD) microparticles into PMMA to improve antibiotic release and allow for post-implantation drug refilling to occur in a tissue-mimicking model. To simulate how antibiotic refilling may be possible in more physiologically relevant models, this work investigated development of bone and muscle refilling models. The bone refilling model involved embedding PMMA-CD into rabbit femur and administering antibiotic via intraosseous infusion. Muscle tissue refilling model involved implanting PMMA-CD beads in bovine muscle tissue and administering antibiotic via tissue injection. Duration of antimicrobial activity of refilled PMMA-CD was evaluated. PMMA-CD composite in bone and muscle tissue models was capable of being refilled with antibiotics and resulted in prolonged antimicrobial activity. PMMA-CD provided sustained and on-demand antimicrobial activity without removal of implant if infection develops. Intraosseous infusion appeared to be a viable technique to enable refilling of PMMA-CD after implantation in bone, reporting for the first time the ability to refill PMMA in bone.
Orographic Factors as a Predictor of the Spread of the Siberian Silk Moth Outbreak in the Mountainous Southern Taiga Forests of Siberia
Authors: Sultson Svetlana M. , Goroshko Andrey A. , Verkhovets Sergey V. , Mikhaylov Pavel V. , Ivanov Valery A. , Demidko Denis A. , Kulakov Sergey S.
Published: January 2021

This research is dedicated to solving an urgent problem associated with the large-scale destruction of taiga forests by Siberian silk moth (Dendrolimus sibiricus) outbreaks. The dynamics of the damage to dark coniferous forest stands induced by the Siberian silk moth outbreaks in mid-altitude mountains were studied. A hypothesis was formulated based on the fundamental influence of the orography on the phytophage’s dispersal within the landscape, along with the climate, which acts as a secondary predictor—a catalyst for outbreaks. The study was carried out using Landsat−8 satellite imagery time-series (from 2018 to 2020). The data were verified using a field forest pathological survey of the territory. An assessment of the defoliated forest area and damage association with the landscape was carried out using an Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) digital elevation model. The assessment was aimed to detail the forecast parameters for an outbreak development in mid-altitude mountains using the orographic features—altitude, terrain slope, and slope aspect. Early warnings of phytophagous insect outbreaks in mountain southern taiga should be focused on the permanent monitoring of dark coniferous stands of the mossy group of forest types, covering altitude levels from 400 to 600 m, located on gentle terrains and slopes of up to 15 degrees. The greatest vulnerability to phytophage impacts was characterized as areas located at altitudes from 400 to 600 m. The upper limit of D. sibiricus distribution was 900 m above sea level. The results obtained provide comprehensive information on the Siberian silk moth potential reserves within the study area with the possibility of extrapolation to similar territories. The data will make it possible to model pest outbreaks based on orography and improve the forest pathological monitoring methods at the regional level.
Rwanda’s Land Policy Reform: Self-Employment Perspectives from a Case Study of Kimonyi Sector
Authors: Mizero Mireille , Maniriho Aristide , Bashangwa Mpozi Bosco , Karangwa Antoine , Burny Philippe , Lebailly Philippe
Published: January 2021

Rwanda’s Land Policy Reform promotes agri-business and encourages self-employment. This paper aims to analyze the situation from a self-employment perspective when dealing with expropriation risk in rural areas. In this study, we conducted a structured survey addressed to 63 domestic units, complemented by focus groups of 47 participants from Kimonyi Sector. The binary logistic regression analysis revealed that having job alternatives, men heading domestic units, literacy skills in English, and owning land lease certificates (p < 0.05) are positively and significantly related to awareness of land expropriation risk. The decision of the head of the domestic unit to practice the main activity under self-employment status is positively influenced by owning a land lease certificate, number of plots, and French skills, while skills in English and a domestic unit’s size have a positive and significant influence on involvement in a second activity as self-employed. Information on expropriation risk has no significant effect on self-employment. The domestic unit survey revealed that 34.9% of the heads of domestic units only have one job, 47.6% have at least two jobs in their everyday life, 12.7% have a minimum of three jobs, and 4.8% are inactive. The focus group synthesis exposed the limits to self-employment ability and facilities.
Decreased Sound Tolerance in Tinnitus Patients
Authors: Raj-Koziak Danuta , Gos Elżbieta , Kutyba Justyna , Skarzynski Henryk , Skarzynski Piotr H.
Published: January 2021

(1) Background: Decreased sound tolerance is a significant problem in tinnitus sufferers. The aim of the study was to evaluate the relationship between tinnitus and decreased sound tolerance (hyperacusis and misophonia). (2) Methods: The study sample consisted of 74 patients with tinnitus and decreased sound tolerance. The procedure comprised patient interviews, pure tone audiometry, impedance audiometry, measurement of uncomfortable loudness levels, and administration of the Hyperacusis Questionnaire, Tinnitus Handicap Inventory, and Visual Analogue Scales. (3) Results: The majority (69%) of the patients reported that noise aggravated their tinnitus. The correlation between tinnitus and hyperacusis was found to be statistically significant and positive: r = 0.44; p < 0.01. The higher the tinnitus severity, the greater the hyperacusis. There was no correlation between misophonia and hyperacusis (r = 0.18; p > 0.05), or between misophonia and tinnitus (r = 0.06; p > 0.05). (4) Conclusions: For tinnitus patients the more significant problem was hyperacusis rather than misophonia. The diagnosis and treatment of decreased sound tolerance should take into account not only audiological, but also psychological problems of the patients.
The Emerging Physiological Role of AGMO 10 Years after Its Gene Identification
Authors: Sailer Sabrina , Keller Markus A. , Werner Ernst R. , Watschinger Katrin
Published: January 2021

The gene encoding alkylglycerol monooxygenase (AGMO) was assigned 10 years ago. So far, AGMO is the only known enzyme capable of catalysing the breakdown of alkylglycerols and lyso-alkylglycerophospholipids. With the knowledge of the genetic information, it was possible to relate a potential contribution for mutations in the AGMO locus to human diseases by genome-wide association studies. A possible role for AGMO was implicated by genetic analyses in a variety of human pathologies such as type 2 diabetes, neurodevelopmental disorders, cancer, and immune defence. Deficient catabolism of stored lipids carrying an alkyl bond by an absence of AGMO was shown to impact on the overall lipid composition also outside the ether lipid pool. This review focuses on the current evidence of AGMO in human diseases and summarises experimental evidence for its role in immunity, energy homeostasis, and development in humans and several model organisms. With the progress in lipidomics platform and genetic identification of enzymes involved in ether lipid metabolism such as AGMO, it is now possible to study the consequence of gene ablation on the global lipid pool and further on certain signalling cascades in a variety of model organisms in more detail.
Psycho-Social Factors in Patients with Cardiovascular Disease Attending a Family-Centred Prevention and Rehabilitation Programme: EUROACTION Model in Spain
Authors: Buigues Cristina , Queralt Ana , De Velasco Jose Antonio , Salvador-Sanz Antonio , Jennings Catriona , Wood David , Trapero Isabel
Published: January 2021

Background: Coronary heart disease (CHD) persists as the leading cause of death worldwide. Cardiovascular prevention and rehabilitation (CVPR) has an interdisciplinary focus, and includes not only in physiological components, but it also addresses psycho-social factors. Methods: The study analysed the Spanish psycho-social data collected during the EUROACTION study. In Spain, two hospitals were randomised in the Valencia community. Coronary patients were prospectively and consecutively identified in both hospitals. The intervention hospital carried out a 16-week CVPR programme, which aimed to assess illness perceptions and establish healthy behaviours in patients and their partners. Results: Illness perceptions were significantly and inversely associated with anxiety and depression. Low levels of anxiety were associated with better self-management of total cholesterol (p = 0.004) and low-density lipoprotein-cholesterol (p = 0.004). There was concordance at one year among patients and partners who participated in the programme related to anxiety (p < 0.001), fruit consumption (p < 0.001), and vegetable consumption (p < 0.001). Conclusions: The EUROACTION study emphasised the importance of assessing psycho-social factors in a CVPR programme and the inclusion of family as support in patients’ changes in behaviour.
Resistance to Growth of Molds for Wood Modified with Hydrophobic Hybrid Silica Gel Containing Copper Amine Complexes
Authors: Hao Shaokun , Hu Chuanshuang , Lin Xiuyi , Gu Jin , Yun Hong , Zhang Weiwei
Published: January 2021

Complexation copper with amine provides an effective strategy for fixation copper in wood, while hydrophobic modification improves the dimensional stability of wood. Thus, a combination of complexation and hydrophobization is expected to enhance the efficiency of copper-based biocides. In this study, hydrophobic hybrid silica gel containing copper amine complexes (MACu) was prepared through an in situ sol-gel process in wood using methyltriethoxysilane (MTES), 3-amino-propyltriethoxysilane (APTES), and copper chloride. The resistance to growth of molds for MACu modified wood (Populus tomentosa) was measured according to ASTM D3273-16. A leaching resistance test was carried out in accordance with AWPA E11-16. The results showed that only Aspergillus niger covered the surface of untreated wood blocks and no mold grew on the MACu surface even after the leaching test. MACu xerogel and MACu wood were further characterized by SEM-EDS, FTIR, and XPS. A possible schematic diagram of the reaction mechanism was proposed to explain the high-efficiency anti-mold performance of MACu wood.
Effect of Rotor Geometry on Bending Stiffness Variation
Authors: Viitala Risto , Tiainen Tuomas , Viitala Raine
Published: January 2021

Bending stiffness variation (BSV) is a common problem causing vibration in large rotating machinery. BSV describes lateral bending stiffness and its variation as a function of the rotational angle. It has been observed that BSV causes excitation exactly twice per revolution, which leads to vibration problems, especially at half critical speed. BSV is caused by rotor geometry errors if the material is assumed to be homogeneous and linearly elastic. Therefore, the study investigated BSV with harmonic roundness components, which are commonly used in industry to describe the geometry of a rotor. Hence, the results are easily applicable in the industry. The research was conducted primarily by analytical means, but also static simulations and numeric calculations were used. The results clearly showed that when the effect of single harmonic roundness components in rotor cross-sections were observed, only the second component could produce BSV. However, when component pairs were studied, they produced BSV also without the second component. If the second component was included, the profile produced BSV the most aggressively. A generated arbitrary roundness profile, including components 3–50 with random phases and amplitudes, indicated that BSV occurs always twice per revolution despite different components in the profile. The results improve the possibilities of eliminating excessive BSV in the industry, when certain components and component pairs can be avoided.
Regular Parameter Elements and Regular Local Hyperrings
Authors: Bordbar Hashem , Cristea Irina
Published: January 2021

Inspired by the concept of regular local rings in classical algebra, in this article we initiate the study of the regular parameter elements in a commutative local Noetherian hyperring. These elements provide a deep connection between the dimension of the hyperring and its primary hyperideals. Then, our study focusses on the concept of regular local hyperring R, with maximal hyperideal M, having the property that the dimension of R is equal to the dimension of the vectorial hyperspace MM2 over the hyperfield RM. Finally, using the regular local hyperrings, we determine the dimension of the hyperrings of fractions.
Acknowledgment to Reviewers of Metals in 2020
Authors:   Metals Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Metals maintains its standards for the high quality of its published papers [...]
Liquid Mixing Based on Electrokinetic Vortices Generated in a T-Type Microchannel
Authors: Wang Chengfa
Published: January 2021

This article proposes a micromixer based on the vortices generated in a T-type microchannel with nonuniform but same polarity zeta potentials under a direct current (DC) electric field. The downstream section (modified section) of the outlet channel was designed with a smaller zeta potential than others (unmodified section). When a DC electric field is applied in the microchannel, the electrokinetic vortices will form under certain conditions and hence mix the solution. The numerical results show that the mixing performance is better when the channel width and the zeta potential ratio of the modified section to the unmodified section are smaller. Besides, the electrokinetic vortices formed in the microchannel are stronger under a larger length ratio of the modified section to the unmodified section of the outlet channel, and correspondingly, the mixing performance is better. The micromixer presented in the paper is quite simple in structure and has good potential applications in microfluidic devices.
Characterizing Fungal Decay of Beech Wood: Potential for Biotechnological Applications
Authors: Bari Ehsan , Ohno Katie , Yilgor Nural , Singh Adya P. , Morrell Jeffrey J. , Pizzi Antonio , Tajick Ghanbary Mohammad Ali , Ribera Javier
Published: January 2021

The biotechnological potential of nine decay fungi collected from stored beech logs at a pulp and paper factory yard in Northern Iran was investigated. Beech blocks exposed to the fungi in a laboratory decay test were used to study changes in cell wall chemistry using both wet chemistry and spectroscopic methods. Pleurotus ostreatus, P. pulmonarius, and Lentinus sajor-caju caused greater lignin breakdown compared to other white-rot fungi, which led to a 28% reduction in refining energy. Trametesversicolor caused the greatest glucan loss, while P. ostreatus and L. sajor-caju were associated with the lowest losses of this sugar. Fourier transform infrared spectroscopy (FTIR) analyses indicated that white-rot fungi caused greater lignin degradation in the cell walls via the oxidation aromatic rings, confirming the chemical analysis. The rate of cellulose and lignin degradation by the T.versicolor and Pleurotus species was high compared to the other decay fungi analyzed in this study. Based on the above information, we propose that, among the fungi tested, P. ostreatus (27.42% lignin loss and 1.58% cellulose loss) and L. sajor-caju (29.92% lignin loss and 5.95% cellulose loss) have the greatest potential for biopulping.
Industrial Verification and Research Development of Lime–Gypsum Fertilizer Granulation Method
Authors: Siuda Robert , Kwiatek Jerzy , Szufa Szymon , Obraniak Andrzej , Piersa Piotr , Adrian Łukasz , Modrzewski Remigiusz , Ławińska Katarzyna , Siczek Krzysztof , Olejnik Tomasz P.
Published: January 2021

This work concerns non-pressure granulation of mineral materials used for the production of agricultural fertilizers for soil deacidification. In order to expand the product range of Nordkalk Poland sp. z o. o. located in Poland, the granulation conditions of the gypsum–lime mix were examined with the use of various granulation methods. The processed mixture was Jurassic lime flour mined in the Sławno mine (Poland) and waste gypsum (sulfogypsum) obtained from the largest coal-fired power plant in the EU, Bełchatów Power Plant (Poland). This paper presents the results of the optimization of the gypsum–lime fertilizer granulation process. The results of the study of granulation of gypsum–lime mixture realized in one-stage technology in a disc granulator were compared with the effects of two-stage agglomeration. During the research, a mixture (in a 1:1 ratio) of waste sulfogypsum and lime flour was used. Such a weight ratio provides maximum use of the sulfogypsum waste while maintaining good mechanical properties of the granulate. The granulated bed was moistened with a lignosulfonate solution. The process was carried out periodically. After the experiment, the grain composition of the granulate obtained was determined and tests were performed to determine the strength of the product. The test results were compared with analogous ones obtained during granulation with the use of molasses (waste from sugar production). The results obtained were verified during a trial carried out on an industrial scale.
Thermo-Mechanical Regime of the Greenland Ice Sheet and Erosion Potential of the Crystalline Bedrock
Authors: Li Zhenze , Nguyen Thanh Son
Published: January 2021

Past glaciation is known to have caused a substantial morphological change to high latitude regions of the northern hemisphere. In the assessment of the long-term performance of deep geological repositories for radioactive wastes, future glaciation is a critical factor to take into consideration. This study develops a thermal-mechanical model to investigate ice sheet thermal evolution and the impact on bedrock erosion. The model is based on comprehensive field data resulting from international collaborative research on the Greenland Analogue Project. The ice sheet model considers surface energy balance and basal heat flux, as well as the temperature-dependent flow of ice that follows Glen’s law. The ice-bedrock interface is treated with a mechanical contact model, which solves the relative velocity and predicts the abrasional erosion and meltwater flow erosion. The numerical model is calibrated with measured temperature profiles and surface velocities at different locations across the glacier cross-section. The erosion rate is substantially larger near the glacier edge, where channel flow erosion becomes predominant. The abrasional erosion rate is averaged at 0.006 mm/a, and peaks at regions near the ridge divide. The mean meltwater flow erosion rate in the study area is estimated to be about 0.12 mm/a for the melted base region.
An Overview of the Potential Therapeutic Applications of Essential Oils
Authors: Aljaafari Mariam Nasser , AlAli Asma Obaid , Baqais Laila , Alqubaisy Maream , AlAli Mudhi , Molouki Aidin , Ong-Abdullah Janna , Abushelaibi Aisha , Lai Kok-Song , Lim Swee-Hua Erin
Published: January 2021

The emergence of antimicrobial resistance (AMR) has urged researchers to explore therapeutic alternatives, one of which includes the use of natural plant products such as essential oils (EO). In fact, EO obtained from clove, oregano, thymus, cinnamon bark, rosemary, eucalyptus, and lavender have been shown to present significant inhibitory effects on bacteria, fungi, and viruses; many studies have been done to measure EO efficacy against microorganisms. The strategy of combinatory effects via conventional and non-conventional methods revealed that the combined effects of EO–EO or EO–antibiotic exhibit enhanced efficacy. This paper aims to review the antimicrobial effects of EO, modes of EO action (membrane disruption, efflux inhibition, increase membrane permeability, and decrease in intracellular ATP), and their compounds’ potential as effective agents against bacteria, fungi, and viruses. It is hoped that the integration of EO applications in this work can be used to consider EO for future clinical applications.
Pulmonaria obscura and Pulmonaria officinalis Extracts as Mitigators of Peroxynitrite-Induced Oxidative Stress and Cyclooxygenase-2 Inhibitors–In Vitro and In Silico Studies
Authors: Krzyżanowska-Kowalczyk Justyna , Kowalczyk Mariusz , Ponczek Michał B. , Pecio Łukasz , Nowak Paweł , Kolodziejczyk-Czepas Joanna
Published: January 2021

The Pulmonaria species (lungwort) are edible plants and traditional remedies for different disorders of the respiratory system. Our work covers a comparative study on biological actions in human blood plasma and cyclooxygenase-2 (COX-2) -inhibitory properties of plant extracts (i.e., phenolic-rich fractions) originated from aerial parts of P. obscura Dumort. and P. officinalis L. Phytochemical profiling demonstrated the abundance of phenolic acids and their derivatives (over 80% of the isolated fractions). Danshensu conjugates with caffeic acid, i.e., rosmarinic, lithospermic, salvianolic, monardic, shimobashiric and yunnaneic acids were identified as predominant components. The examined extracts (1–100 µg/mL) partly prevented harmful effects of the peroxynitrite-induced oxidative stress in blood plasma (decreased oxidative damage to blood plasma components and improved its non-enzymatic antioxidant capacity). The cellular safety of the extracts was confirmed in experimental models of blood platelets and peripheral blood mononuclear cells. COX-2 inhibitor screening evidently suggested a stronger activity of P. officinalis (IC50 of 13.28 and 7.24 µg/mL, in reaction with synthetic chromogen and physiological substrate (arachidonic acid), respectively). In silico studies on interactions of main components of the Pulmonaria extracts with the COX-2 demonstrated the abilities of ten compounds to bind with the enzyme, including rosmarinic acid, menisdaurin, globoidnan A and salvianolic acid H.
Current Updates on Naturally Occurring Compounds Recognizing SARS-CoV-2 Druggable Targets
Authors: Romeo Isabella , Mesiti Francesco , Lupia Antonio , Alcaro Stefano
Published: January 2021

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified in China as the etiologic agent of the recent COVID-19 pandemic outbreak. Due to its high transmissibility, this virus quickly spread throughout the world, causing considerable health issues. The scientific community exerted noteworthy efforts to obtain therapeutic solutions for COVID-19, and new scientific networks were constituted. No certified drugs to efficiently inhibit the virus were identified, and the development of de-novo medicines requires approximately ten years of research. Therefore, the repurposing of natural products could be an effective strategy to handle SARS-CoV-2 infection. This review aims to update on current status of the natural occurring compounds recognizing SARS-CoV-2 druggable targets. Among the clinical trials actually recruited, some natural compounds are ongoing to examine their potential role to prevent and to treat the COVID-19 infection. Many natural scaffolds, including alkaloids, terpenes, flavonoids, and benzoquinones, were investigated by in-silico, in-vitro, and in-vivo approaches. Despite the large data set obtained by a computational approach, experimental evidences in most cases are not available. To fill this gap, further efforts to validate these results are required. We believe that an accurate investigation of naturally occurring compounds may provide insights for the potential treatment of COVID-19 patients.
Impact of Different Levels of Supervision on the Recovery of Severely Malnourished Children Treated by Community Health Workers in Mali
Authors: Charle-Cuéllar Pilar , López-Ejeda Noemí , Traore Mamadou , Coulibaly Adama Balla , Landouré Aly , Diawara Fatou , Bunkembo Magloire , Vargas Antonio , Gil Ruth , Briend André
Published: January 2021

(1) Background: The Ministry of Health in Mali included the treatment of severe acute malnutrition (SAM) into the package of activities of the integrated community case management (iCCM). This paper evaluates the most effective model of supervision for treating SAM using community health workers (CHWs). Methods (2): This study was a prospective non-randomized community intervention trial with two intervention groups and one control group with different levels of supervision. It was conducted in three districts in rural areas of the Kayes Region. In the high supervision group, CHWs received supportive supervision for the iCCM package and nutrition-specific supervision. In the light supervision group, CHWs received supportive supervision based on the iCCM package. The control group had no specific supervision. (3) Results: A total of 6112 children aged 6–59 months with SAM without medical complications were included in the study. The proportion of cured children was 81.4% in those treated by CHWs in the high supervision group, 86.2% in the light supervision group, and 66.9% in the control group. Children treated by the CHWs who received some supervision had better outcomes than those treated by unsupervised CHWs (p < 0.001). There was no difference between areas with light and high supervision, although those with high supervision performed better in most of the tasks analyzed. (4) Conclusions: Public policies in low-income countries should be adapted, and their model of supervision of CHWs for SAM treatment in the community should be evaluated.
Acknowledgment to Reviewers of Nutrients in 2020
Authors:   Nutrients Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Nutrients maintains its standards for the high quality of its published papers [...]
Association of the Omega-3 Index with Incident Prostate Cancer with Updated Meta-Analysis: The Cooper Center Longitudinal Study
Authors: Farrell Stephen W. , DeFina Laura F. , Tintle Nathan L. , Leonard David , Cooper Kenneth H. , Barlow Carolyn E. , Haskell William L. , Pavlovic Andjelka , Harris William S.
Published: January 2021

Background: The association between long-chain omega-3 polyunsaturated fatty acids (n-3 PUFA) and prostate cancer (PC) remains unclear. Methods: We compared incident PC rates as a function of the Omega-3 Index [O3I, erythrocyte eicosapentaenoic and docosahexaenoic acids (EPA + DHA)] in 5607 men (40–80 years of age) seen at the Cooper Clinic who were free of PC at baseline. The average follow-up was 5.1 ± 2.8 years until censoring or reporting a new PC diagnosis. Proportional hazards regression was used to model the linear association between baseline O3I and the age-adjusted time to diagnosis. A meta-analysis of n-3 PUFA biomarker-based studies and incident PC was updated with the present findings. Results: A total of 116 cases of incident PC were identified. When O3I was examined as a continuous variable, the age-adjusted hazard ratio (HR) (95% CI) was 0.98 (0.89, 1.07; p = 0.25) for each 1% increment in the O3I. The updated meta-analysis with 10 biomarker-based studies found no significant relationship between EPA or DHA levels and risk for PC. Conclusions: We find no evidence in this study nor in a meta-analysis of similar studies that consuming n-3 PUFA-rich fish or using fish oil supplements affects the risk of PC.