bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation on the Nutrition Quotient Scores of Elderly People Living Alone in Korea
Authors: Gang Gyoungok , Lee Min , Choi Eun-hui , Lee Hye-Lim , Lee Hyun-Young , Chang Hye-Ja , Choi Jung-Hwa , Yi Na-Young , Lee Kyung-Eun , Chung Min-Jae , Kwak Tong-Kyung
Published: August 2023

As the Korean society is aging rapidly, the issues on physical, social, economic, and mental disabilities of single-person households aged 65 years or older has also increased. This study aimed to investigate the nutrition-related dietary conditions of elderly people living alone and determine their dietary behavior by calculating the nutrition quotient for elderly (NQ-E). One hundred and three elderly people living alone who were basic living recipients were recruited from six senior welfare centers in Seoul, and the data were collected using a questionnaire from 19 July 2016 to 17 August 2016, with a 1:1 in-depth interview using the modified version of the NQ-E questionnaire. The data were analyzed using SPSS 27.0 for Mac (IBM Corp., Armonk, NY, USA); a p value of <0.05 was considered significant. The nutrition-related dietary conditions of the elderly living alone were limited, and many of them received support from the government, which helped improve their diet. The nutrition quotient score of the elderly living alone was 50.14, which was lower than the NQ-E mean score (57.6) of the Korean elderly and the NQ-E (62 points), which is the top 25% of the national survey subjects according to the criteria value presented by the Korean Nutrition Society. Elderly people living alone often have poor dietary habits and nutritional status. The NQ-E presented in this study can be used to evaluate the dietary conditions of the elderly and is expected to be used as an indicator for developing community programs for health promotion and evaluating their effectiveness.
Phylogeographic Aspects of Bat Lyssaviruses in Europe: A Review
Authors: Dundarova Heliana , Ivanova-Aleksandrova Nadya , Bednarikova Sarka , Georgieva Irina , Kirov Krasimir , Miteva Kalina , Neov Boyko , Ostoich Peter , Pikula Jiri , Zukal Jan , Hristov Peter
Published: August 2023

During the last few decades, bat lyssaviruses have become the topic of intensive molecular and epidemiological investigations. Since ancient times, rhabdoviruses have caused fatal encephalitis in humans which has led to research into effective strategies for their eradication. Modelling of potential future cross-species virus transmissions forms a substantial component of the recent infection biology of rabies. In this article, we summarise the available data on the phylogeography of both bats and lyssaviruses in Europe and the adjacent reg ions, especially in the contact zone between the Palearctic and Ethiopian realms. Within these zones, three bat families are present with high potential for cross-species transmission and the spread of lyssaviruses in Phylogroup II to Europe (part of the western Palearctic). The lack of effective therapies for rabies viruses in Phylogroup II and the most divergent lyssaviruses generates impetus for additional phylogenetic and virological research within this geographical region.
Xenorhabdus and Photorhabdus Bacteria as Potential Candidates for the Control of Culex pipiens L. (Diptera: Culicidae), the Principal Vector of West Nile Virus and Lymphatic Filariasis
Authors: Yüksel Ebubekir , Yıldırım Alparslan , İmren Mustafa , Canhilal Ramazan , Dababat Abdelfattah A.
Published: August 2023

Vector-borne diseases pose a severe threat to human and animal health. Culex pipiens L. (Diptera: Culicidae) is a widespread mosquito species and serves as a vector for the transmission of infectious diseases such as West Nile disease and Lymphatic Filariasis. Synthetic insecticides have been the prime control method for many years to suppress Cx. pipiens populations. However, recently, the use of insecticides has begun to be questioned due to the detrimental impact on human health and the natural environment. Therefore, many authorities urge the development of eco-friendly control methods that are nontoxic to humans. The bacterial associates [Xenorhabdus and Photorhabdus spp. (Enterobacterales: Morganellaceae)] of entomopathogenic nematodes (EPNs) (Sterinernema spp. and Heterorhabditis spp.) (Rhabditida: Heterorhabditidae and Steinernematidae) are one of the green approaches to combat a variety of insect pests. In the present study, the mosquitocidal activity of the cell-free supernatants and cell suspension (4 × 107 cells mL−1) of four different symbiotic bacteria (Xenorhabdus nematophila, X. bovienii, X. budapestensis, and P. luminescens subsp. kayaii) was assessed against different development stages of Cx. pipiens (The 1st/2nd and 3rd/4th instar larvae and pupa) under laboratory conditions. The bacterial symbionts were able to kill all the development stages with varying levels of mortality. The 1st/2nd instar larvae exhibited the highest susceptibility to the cell-free supernatants and cell suspensions of symbiotic bacteria and the efficacy of the cell-free supernatants and cell suspensions gradually declined with increasing phases of growth. The highest effectiveness was achieved by the X. bovienii KCS-4S strain inducing 95% mortality to the 1st/2nd instar larvae. The results indicate that tested bacterial symbionts have great potential as an eco-friendly alternative to insecticides.
Imidazoles as Serotonin Receptor Modulators for Treatment of Depression: Structural Insights and Structure–Activity Relationship Studies
Authors: Goel Kapil Kumar , Thapliyal Somesh , Kharb Rajeev , Joshi Gaurav , Negi Arvind , Kumar Bhupinder
Published: August 2023

Serotoninergic signaling is identified as a crucial player in psychiatric disorders (notably depression), presenting it as a significant therapeutic target for treating such conditions. Inhibitors of serotoninergic signaling (especially selective serotonin reuptake inhibitors (SSRI) or serotonin and norepinephrine reuptake inhibitors (SNRI)) are prominently selected as first-line therapy for the treatment of depression, which benefits via increasing low serotonin levels and norepinephrine by blocking serotonin/norepinephrine reuptake and thereby increasing activity. While developing newer heterocyclic scaffolds to target/modulate the serotonergic systems, imidazole-bearing pharmacophores have emerged. The imidazole-derived pharmacophore already demonstrated unique structural characteristics and an electron-rich environment, ultimately resulting in a diverse range of bioactivities. Therefore, the current manuscript discloses such a specific modification and structural activity relationship (SAR) of attempted derivatization in terms of the serotonergic efficacy of the resultant inhibitor. We also featured a landscape of imidazole-based development, focusing on SAR studies against the serotoninergic system to target depression. This study covers the recent advancements in synthetic methodologies for imidazole derivatives and the development of new molecules having antidepressant activity via modulating serotonergic systems, along with their SAR studies. The focus of the study is to provide structural insights into imidazole-based derivatives as serotonergic system modulators for the treatment of depression.
Characterization and In Vivo Antiangiogenic Activity Evaluation of Morin-Based Cyclodextrin Inclusion Complexes
Authors: De Gaetano Federica , Margani Fatima , Barbera Vincenzina , D’Angelo Valeria , Germanò Maria Paola , Pistarà Venerando , Ventura Cinzia Anna
Published: August 2023

Morin (MRN) is a natural compound with antiangiogenic, antioxidant, anti-inflammatory, and anticancer activity. However, it shows a very low water solubility (28 μg/mL) that reduces its oral absorption, making bioavailability low and unpredictable. To improve MRN solubility and positively affect its biological activity, particularly its antiangiogenic activity, in this work, we prepared the inclusion complexes of MNR with sulfobutylether-β-cyclodextrin (SBE-β-CD) and hydroxypropyl-β-cyclodextrin (HP-β-CD). The inclusion complexes obtained by the freeze-drying method were extensively characterized in solution (phase-solubility studies, UV–Vis titration, and NMR spectroscopy) and in the solid state (TGA, DSC, and WAXD analysis). The complexation significantly increased the water solubility by about 100 times for MRN/HP-β-CD and 115 times for MRN/SBE-β-CD. Furthermore, quantitative dissolution of the complexes was observed within 60 min, whilst 1% of the free drug dissolved in the same experimental time. 1H NMR and UV–Vis titration studies demonstrated both CDs well include the benzoyl moiety of the drug. Additionally, SBE-β-CD could interact with the cinnamoyl moiety of MRN too. The complexes are stable in solution, showing a high value of association constant, that is, 3380 M−1 for MRN/HP-β-CD and 2870 M−1 for MRN/SBE-β-CD. In vivo biological studies on chick embryo chorioallantoic membrane (CAM) and zebrafish embryo models demonstrated the high biocompatibility of the inclusion complexes and the effective increase in antiangiogenic activity of complexed MRN with respect to the free drug.
CNP-miR146a Decreases Inflammation in Murine Acute Infectious Lung Injury
Authors: Vaughn Alyssa E. , Lehmann Tanner , Sul Christina , Wallbank Alison M. , Lyttle Bailey D. , Bardill James , Burns Nana , Apte Anisha , Nozik Eva S. , Smith Bradford , Vohwinkel Christine U. , Zgheib Carlos , Liechty Kenneth W.
Published: August 2023

Acute respiratory distress syndrome (ARDS) has approximately 40% in-hospital mortality, and treatment is limited to supportive care. Pneumonia is the underlying etiology in many cases with unrestrained inflammation central to the pathophysiology. We have previously shown that CNP-miR146a, a radical scavenging cerium oxide nanoparticle (CNP) conjugated to the anti-inflammatory microRNA(miR)-146a, reduces bleomycin- and endotoxin-induced acute lung injury (ALI) by decreasing inflammation. We therefore hypothesized that CNP-miR146a would decrease inflammation in murine infectious ALI. Mice were injured with intratracheal (IT) MRSA or saline followed by treatment with IT CNP-miR146a or saline control. Twenty-four hours post-infection, bronchoalveolar lavage fluid (BALF) and whole lungs were analyzed for various markers of inflammation. Compared to controls, MRSA infection significantly increased proinflammatory gene expression (IL-6, IL-8, TNFα, IL-1β; p < 0.05), BALF proinflammatory cytokines (IL-6, IL-8, TNFα, IL-1β; p < 0.01), and inflammatory cell infiltrate (p = 0.03). CNP-miR146a treatment significantly decreased proinflammatory gene expression (IL-6, IL-8, TNFα, IL-1β; p < 0.05), bronchoalveolar proinflammatory protein leak (IL-6, IL-8, TNFα; p < 0.05), and inflammatory infiltrate (p = 0.01). CNP-miR146a decreases inflammation and improves alveolar–capillary barrier integrity in the MRSA-infected lung and has significant promise as a potential therapeutic for ARDS.
Cocrystals Enhance Biopharmaceutical and Antimicrobial Properties of Norfloxacin
Authors: Gomes Samantha Nascimento , Biscaia Isabela Fanelli Barreto , Lopes Diana Schon , Mengarda Mariana , Murakami Fábio Seigi , Oliveira Paulo Renato , Bernardi Larissa Sakis
Published: August 2023

A solvate cocrystal of the antimicrobial norfloxacin (NFX) was formed by using isonicotinamide (INA) as a coformer with the solvent evaporation technique. The cocrystal formation was confirmed by performing solid-state characterization techniques. We evaluated the dissolution under supersaturated conditions and also the solubility at the vertex of triphasic domain of cocrystal and NFX in both water and Fasted-State Simulated Intestinal Fluid (FaSSIF). The antimicrobial activity was evaluated using the microdilution technique. The cocrystal showed 1.8 times higher dissolution than NFX in water at 60 min and 1.3 times higher in FaSSIF at 180 min in the kinetic study. The cocrystal also had an increase in solubility of 8.38 times in water and 6.41 times in FaSSIF. The biopharmaceutical properties of NFX with cocrystallization improved antimicrobial action, as shown in the results of minimum inhibitory concentration (MIC) and inhibitory concentrations of 50% (IC50%) and 90% (IC90%). This paper presents, for the first time, a more in-depth analysis of the cocrystal of NFX–INA concerning its dissolution, solubility, and antimicrobial activity. In all these criteria, the cocrystal obtained better results compared to the pure drug.
Do Mixtures of Beads with Different Sizes Improve Wet Stirred Media Milling of Drug Suspensions?
Authors: Guner Gulenay , Mehaj Mirsad , Seetharaman Natasha , Elashri Sherif , Yao Helen F. , Clancy Donald J. , Bilgili Ecevit
Published: August 2023

The impacts of bead sizes and bead mixtures on breakage kinetics, the number of milling cycles applied to prevent overheating, and power consumption during the nanomilling of drug (griseofulvin) suspensions were investigated from both an experimental and theoretical perspective. Narrowly sized zirconia beads with nominal sizes of 100, 200, and 400 µm and their half-and-half binary mixtures were used at 3000 and 4000 rpm with two bead loadings of 0.35 and 0.50. Particle size evolution was measured during the 3 h milling experiments using laser diffraction. An nth-order breakage model was fitted to the experimental median particle size evolution, and various microhydrodynamic parameters were calculated. In general, the beads and their mixtures with smaller median sizes achieved faster breakage. While the microhydrodynamic model explained the impacts of process parameters, it was limited in describing bead mixtures. For additional test runs performed, the kinetics model augmented with a decision tree model using process parameters outperformed that augmented with an elastic-net regression model using the microhydrodynamic parameters. The evaluation of the process merit scores suggests that the use of bead mixtures did not lead to notable process improvement; 100 µm beads generally outperformed bead mixtures and coarser beads in terms of fast breakage, low power consumption and heat generation, and low intermittent milling cycles.
Milnacipran Has an Antihyperalgesic Effect on Cisplatin-Induced Neuropathy
Authors: Cho Sun Jin , Lee Jin Young , Jeong Yujin , Cho So Yeon , Lee Do-Gyeong , Choi Ji Yeon , Park Hue Jung
Published: August 2023

(1) Background: Milnacipran is a typical serotonin–norepinephrine reuptake inhibitor and has been shown to have analgesic effects in several pain models. However, its antihyperalgesic effect in cisplatin-induced neuropathy remains unknown. We examined the effects of intraperitoneal (IP) milnacipran on allodynia in cisplatin-induced peripheral neuropathic mice. (2) Methods: Peripheral neuropathy was induced by injecting cisplatin (2.3 mg/kg/day, IP) six times, on every other day. Saline or milnacipran (10, 30, 50 mg/kg, IP) were then administered to the neuropathic mice. We examined mechanical allodynia using von Frey hairs at preadministration and at 30, 60, 90, 120, 180, 240 min and 24 h after drug administration. We also measured the dorsal root ganglion (DRG) activating transcription factor 3 (ATF3) to confirm the analgesic effects of milnacipran. (3) Results: For the milnacipran groups, the decreased paw withdrawal thresholds to mechanical stimuli were significantly reversed when compared to the preadministration values and the values in the saline-injected control group (p < 0.0001). Milnacipran administration to cisplatin-induced peripheral neuropathic mice resulted in a significant suppression of neuronal ATF3 activation (p < 0.01). (4) Conclusions: Milnacipran given via IP injection attenuates mechanical allodynia in mouse models of cisplatin-induced poly-neuropathic pain. These effects were confirmed by significant suppression of neuronal ATF3 activation in the DRG.
Influence of Manufacturing Process on the Microstructure, Stability, and Sensorial Properties of a Topical Ointment Formulation
Authors: Chow Pui Shan , Lim Ron Tau Yee , Cyriac Febin , Shah Jaymin C. , Badruddoza Abu Zayed Md , Yeoh Thean , Yagnik Chetan Kantilal , Tee Xin Yi , Wong Annie Bao Hua , Chia Vernissa Dilys , Wang Guan
Published: August 2023

The manufacturing process for ointments typically involves a series of heating, cooling, and mixing steps. Precise control of the level of mixing through homogenization and the cooling rate, as well as temperature at different stages, is important in delivering ointments with the desired quality attributes, stability, and performance. In this work, we investigated the influence of typical plant processing conditions on the microstructure, stability, and sensorial properties of a model ointment system through a Design of Experiments (DoE) approach. Homogenization speed at the cooling stage after the addition of the solvent (propylene glycol, PG) was found to be the critical processing parameter that affects stability and the rheological and sensorial properties of the ointment. A lower PG addition temperature was also found to be beneficial. The stabilization of the ointment at a lower PG addition temperature was hypothesized to be due to more effective encapsulation by crystallizing mono- and diglycerides at the lower temperature. The in vitro release profiles were found to be not influenced by the processing parameters, suggesting that for the ointment platform studied, processing affects the microstructure, but the effects do not translate into the release profile, a key performance indicator. Our systematic study represents a Quality-by-Design (QbD) approach to the design of a robust manufacturing process for delivering stable ointments with the desired performance attributes and properties.
Relative Humidity Optical Sensor Based on Self-Assembled Gold Nanoparticles Covered with Nafion
Authors: Sgibnev Yevgeniy , Tananaev Peter , Shelaev Artem , Yankovskii Georgiy , Baryshev Alexander
Published: August 2023

The detection of humidity plays a vital role in healthcare, industrial, and scientific areas, and the development of an ideal sensor is in continuous progress. In this work, a relative humidity (RH) optical sensor based on localized surface plasmon resonance of self-assembled gold nanoparticles formed by thermal dewetting and coated with Nafion fluoropolymer is under study. Sensor performance has been found to substantially depend on Nafion layer thickness. The best sensing element—an array of gold nanoparticles covered with a 300 nm-thick Nafion—has been shown to possess a linear response in a wide dynamic range of 0–85% RH with a limit of detection down to 0.12%. Thus, a simple and low-cost method for high-accuracy RH detection has been demonstrated.
Nanophotonics Pioneer: Prof. Dr. Dieter Bimberg “Green Photonic Network: From VCSELs to Nanophotonics”
Authors: Shi Jin-Wei , Lu Tien-Chang , Koyama Fumio
Published: August 2023

We would like to celebrate Prof [...]
Chiral Quasi-Bound States in the Continuum of a Dielectric Metasurface for Optical Monitoring and Temperature Sensing
Authors: Du Xu , Xie Suxia , Nan Haoxuan , Sun Siyi , Shen Weiwei , Yang Jingcheng , Guan Xin
Published: August 2023

Chiral BIC can reach ultrahigh quality factors (Q-factor) based on its asymmetry, with broken mirror symmetries and in-plane inversion. Only by in-plane structural perturbation can chiral quasi-BIC (q-BIC) appear, so it is much more realizable and reasonable for the manufacturers in practical productions and fabrications considering the technology and means that are available. In this paper, we design a new dielectric metasurface employing H-shaped silica meta-atoms in the lattice, which is symmetrical in structure, obtaining chiral BIC with ultrahigh Q-factor (exceeding 105). In this process, we change the length of the limbs of the structure to observe the specific BICs. Previous scholars have focused on near-infrared-wavelength bands, while we concentrate on the terahertz wavelength band (0.8–1 THz). We found that there is more than one BIC, thus realizing multiple BICs in the same structure; all of them exhibit excellent circular dichroism (CD) (the maximum value of CD is up to 0.8127) for reflectance and transmittance, which provides significant and unique guidance for the design of multi-sensors. Meanwhile, we performed temperature sensing with chiral BIC; the sensitivity for temperature sensing can reach 13.5 nm/°C, which exhibits high accuracy in measuring temperature. As a consequence, the result proposed in this study will make some contributions to advanced optical imaging, chiral sensors with high frequency and spectral resolution, optical monitoring of environmental water quality, multiple sensors, temperature sensing, biosensing, substance inspection and ambient monitoring and other relevant optical applications.
Genome-Wide Identification, Phylogeny and Expression Analysis of Subtilisin (SBT) Gene Family under Wheat Biotic and Abiotic Stress
Authors: Zhao Xiaotong , Goher Farhan , Chen Lei , Song Jiancheng , Zhao Jiqiang
Published: August 2023

The subtilisin-like protease (SBT) family is widely known for its role in stress resistance to a number of stressors in different plant species, but is rarely studied in wheat. Subtilisin-like serine proteases (SBTs) are serine proteolytic enzymes that hydrolyze proteins into small peptides, which bind to receptors as signal molecules or ligands and participate in signal transduction. In this study, we identified 255 putative SBT genes from the wheat reference genome and then divided these into seven clades. Subsequently, we performed syntenic relation analysis, exon-intron organization, motif composition, and cis-element analysis. Further, expression analysis based on RNA-seq and tissue-specific expression patterns revealed that TaSBT gene family expression has multiple intrinsic functions during various abiotic and biotic stresses. Analysis of RNA-seq expression assays and further validation through qRT PCR suggested that some of the TaSBT genes have significant changes in expression levels during Pst interaction. TaSBT7, TaSBT26, TaSBT102, and TaSBT193 genes showed increasing expression levels during compatible and non-compatible interactions, while the expression levels of TaSBT111 and TaSBT213 showed a decreasing trend, indicating that these members of the wheat SBT gene family may have a role in wheat’s defense against pathogens. In conclusion, these results expand our understanding of the SBT gene family, and provide a valuable reference for future research on the stress resistance function and comprehensive data of wheat SBT members.
The Impact and Invasive Mechanisms of Pueraria montana var. lobata, One of the World’s Worst Alien Species
Authors: Kato-Noguchi Hisashi
Published: August 2023

Pueraria montana var. lobata is native to East Asia, and was introduced to many countries due to its potential for multiple uses. This species escaped under the management conditions soon after its introduction, and became a harmful weed species. This species has been listed in the top 100 of the world’s worst invasive alien species. P. montana stands expand quickly and threaten the native flora and fauna including microbiota. This species affects the concentration of carbon and nitrogen in soil and aquatic environments, and increases the amount of pollutants in the local atmosphere. Its infestation also causes serious economic losses on forestry and agriculture. Its characteristics of fast growth, thick canopy structure, enormous vegetative reproduction, and adaptative ability to the various environmental conditions may contribute to the invasiveness and naturalization of this species. The characteristics of P. montana regarding their defense functions against their natural enemies and pathogens, and allelopathy may also contribute to the invasiveness of this species. Potential allelochemicals such as xanthoxins, p-coumaric acid, caffeic acid, methyl caffeate and daidzein, and two isoflavones with anti-virus activity were identified in this species. In addition, fewer herbivore insects were found in the introduced ranges. These characteristics of P. montana may be involved in the invasive mechanisms of the species. This is the first review article focusing on the invasive mechanisms of this species.
Chemical Composition of Essential Oils from Eight Tunisian Eucalyptus Species and Their Antifungal and Herbicidal Activities
Authors: Ayed Amira , Polito Flavio , Mighri Hedi , Souihi Mouna , Caputo Lucia , Hamrouni Lamia , Amri Ismail , Nazzaro Filomena , De Feo Vincenzo , Hirsch Ann M. , Mabrouk Yassine
Published: August 2023

Eucalyptus species are known to produce metabolites such as essential oils (EOs) that play an important role in the control of weeds, pests and phytopathogenic fungi. The aims of this study were as follows: (i) to determine the chemical composition of the EOs derived from eight Eucalyptus species growing in Tunisia, and (ii) to study their possible antifungal and herbicidal activities. EOs were obtained by hydrodistillation from the dried leaves of eight Eucalyptus species, namely, E. angulosa, E. cladocalyx, E. diversicolor, E. microcoryx, E. ovata, E. resinifera, E. saligna and E. sargentii, and the determination of their composition was achieved by GC and GC-MS. The EOs’ antifungal activities were tested against four Fusarium strains, and the EOs’ herbicidal properties were evaluated on the germination and seedling growth of three annual weeds (Trifolium campestre, Lolium rigidum and Sinapis arvensis) and three cultivated crop species (Lepidium sativum, Raphanus sativus and Triticum durum). The EO yields ranged between 0.12 and 1.32%. The most abundant components found were eucalyptol, α-pinene, p-cymene, trans-pinocarveol, α-terpineol and globulol. All EOs showed significant antifungal activity against the four phytopathogenic Fusarium strains. E. cladocalyx EO exhibited the highest level of antifungal activity, and the greatest inhibition of seed germination was obtained even at lowest concentrations used. These findings suggested that E. resinifera, E. ovata and E. cladocalyx EOs could have applications in agriculture as possible biopesticides, as Fusarium antagonists and as bioherbicides.
Small RNA-Seq to Unveil the miRNA Expression Patterns and Identify the Target Genes in Panax ginseng
Authors: Liu Chang , Jiang Yang , Yun Ziyi , Zhang Kexin , Zhao Mingzhu , Wang Yi , Zhang Meiping , Tian Zhuo , Wang Kangyu
Published: August 2023

Panax ginseng, renowned for its medicinal properties, relies on adventitious roots and hairy roots as crucial sources for the production of ginsenosides. Despite the widespread utilization of ginseng, investigations into its miRNAs have remained scarce. To address this gap, two samples of ginseng adventitious roots and ginseng hairy roots were collected, and subsequent construction and sequencing of small RNA libraries of ginseng adventitious roots and hairy roots were performed using the Illumina HiSeq X Ten platform. The analysis of the sequencing data unveiled total miRNAs 2432. The miR166 and miR396 were the most highly expressed miRNA families in ginseng. The miRNA expression analysis results were used to validate the qRT-PCR. Target genes of miRNA were predicted and GO function annotation and KEGG pathway analysis were performed on target genes. It was found that miRNAs are mainly involved in synthetic pathways and biological processes in plants, which include metabolic and bioregulatory processes. The plant miRNAs enriched KEGG pathways are associated with some metabolism, especially amino acid metabolism and carbohydrate metabolism. These results provide valuable insights miRNAs and their roles in metabolic processes in ginseng.
Automatic Evaluation of Soybean Seed Traits Using RGB Image Data and a Python Algorithm
Authors: Ghimire Amit , Kim Seong-Hoon , Cho Areum , Jang Naeun , Ahn Seonhwa , Islam Mohammad Shafiqul , Mansoor Sheikh , Chung Yong Suk , Kim Yoonha
Published: August 2023

Soybean (Glycine max) is a crucial legume crop known for its nutritional value, as its seeds provide large amounts of plant protein and oil. To ensure maximum productivity in soybean farming, it is essential to carefully choose high-quality seeds that possess desirable characteristics, such as the appropriate size, shape, color, and absence of any damage. By studying the relationship between seed shape and other traits, we can effectively identify different genotypes and improve breeding strategies to develop high-yielding soybean seeds. This study focused on the analysis of seed traits using a Python algorithm. The seed length, width, projected area, and aspect ratio were measured, and the total number of seeds was calculated. The OpenCV library along with the contour detection function were used to measure the seed traits. The seed traits obtained through the algorithm were compared with the values obtained manually and from two software applications (SmartGrain and WinDIAS). The algorithm-derived measurements for the seed length, width, and projected area showed a strong correlation with the measurements obtained using various methods, with R-square values greater than 0.95 (p < 0.0001). Similarly, the error metrics, including the residual standard error, root mean square error, and mean absolute error, were all below 0.5% when comparing the seed length, width, and aspect ratio across different measurement methods. For the projected area, the error was less than 4% when compared with different measurement methods. Furthermore, the algorithm used to count the number of seeds present in the acquired images was highly accurate, and only a few errors were observed. This was a preliminary study that investigated only some morphological traits, and further research is needed to explore more seed attributes.
Harvesting Mycelial Biomass of Selected Basidiomycetes for Chitosan Biopolymer Extraction
Authors: Irbe Ilze , Andze Laura , Blumfelde Mara , Filipova Inese , Verovkins Anrijs , Zoldners Juris
Published: August 2023

This study investigates the mycelial biomass production and chitosan extraction potential of various Basidiomycota strains, including Heterobasidion annosum, Phanerochaete chrysosporium, Pleurotus ostreatus, Trametes versicolor, and Lentinus lepideus. Both submerged fermentation (SF) and solid-state fermentation (SSF) methods were employed. The chitosan yield in basidiocarps of Pleurotus ostreatus, Agaricus bisporus, and Ganoderma applanatum was also evaluated as a reference material. The chitosan extracted from fungal cells was characterized using elemental analyses and FTIR spectroscopy. Among the cultivated strains, P. chrysosporium exhibited the highest mycelial biomass concentration in SF (1.03 g 100 mL–1) after 14 days, while T. versicolor achieved the highest biomass concentration in SSF (3.65 g 100 mL–1). The highest chitosan yield was obtained from the mycelium of P. chrysosporium (0.38%) and T. versicolor (0.37%) in shaken SF. Additionally, commercially cultivated A. bisporus demonstrated the highest chitosan yield in fungal fruiting bodies (1.7%). The extracted chitosan holds potential as a functional biopolymer additive for eco-friendly materials, serving as an alternative to synthetic wet and dry strength agents in packaging materials.
Synthesis and Characterization of Organo-Soluble Polyimides Based on Polycondensation Chemistry of Fluorene-Containing Dianhydride and Amide-Bridged Diamines with Good Optical Transparency and Glass Transition Temperatures over 400 °C
Authors: Ren Xi , Wang Zhenzhong , He Zhibin , Yang Changxu , Qi Yuexin , Han Shujun , Chen Shujing , Yu Haifeng , Liu Jingang
Published: August 2023

Polymeric optical films with light colors, good optical transparency and high thermal resistance have gained increasing attention in advanced optoelectronic areas in recent years. However, it is somewhat inter-conflicting for achieving the good optical properties to the conventional thermal resistant polymers, such as the standard aromatic polyimide (PI) films, which are well known for the excellent combined properties and also the deep colors. In this work, a series of wholly aromatic PI films were prepared via the polycondensation chemistry of one fluorene-containing dianhydride, 9,9-bis(3,4-dicarboxyphenyl)fluorene dianhydride (FDAn) and several aromatic diamines with amide linkages in the main chain, including 9,9-bis [4-(4-aminobenzamide)phenyl]fluorene (FDAADA), 2,2′-bis(trifluoromethyl)-4,4′-bis[4-(4-aminobenzamide)] biphenyl (ABTFMB), and 2,2′-bis(trifluoromethyl)-4,4′-bis[4-(4-amino-3-methyl)benzamide] biphenyl (MABTFMB). The derived FLPI-1 (FDAn-FDAADA), FLPI-2 (FDAn-ABTFMB) and FLPI-3 (FDAn-MABTFMB) resins showed good solubility in the polar aprotic solvents, such as N-methyl-2-pyrrolidone (NMP), N,N-dimethylacetamide (DMAc) and dimethyl sulfoxide (DMSO). The solution-processing FDAn-PI films exhibited good optical transmittance over 80.0% at a wavelength of 500 nm (T500), yellow indices (b*) in the range of 1.01–5.20, and haze values lower than 1.0%. In addition, the FDAn-PI films showed low optical retardance with optical retardation (Rth) values in the range of 31.7–390.6 nm. At the same time, the FDAn-PI films exhibited extremely high glass transition temperatures (Tg) over 420 °C according to dynamic mechanical analysis (DMA) tests. The FDAn-PI films showed good dimensional stability at elevated temperatures with linear coefficients of thermal expansion (CTE) in the range of (31.8–45.8) × 10−6/K.
Isinglass as an Alternative Biopolymer Membrane for Green Electrochemical Devices: Initial Studies of Application in Electric Double-Layer Capacitors and Future Perspectives
Authors: Jeżowski Paweł , Kowalczewski Przemysław Łukasz
Published: August 2023

The presented work discusses in detail the preparation of a cheap and environmentally friendly biopolymer membrane from isinglass and its physicochemical characterisation. One of the possible uses of the obtained membrane can be as a separator between electrodes in novel green electrochemical devices as in, for example, electric double-layer capacitors (EDLCs). The functionality of the mentioned membrane was investigated and demonstrated by classical electrochemical techniques such as cyclic voltammetry (CV), galvanostatic cycling with potential limitation (GCPL), and electrochemical impedance spectroscopy (EIS). The obtained values of capacitance (approximately 30 F g−1) and resistance (approximately. 3 Ohms), as well as the longevity of the EDLC during electrochemical floating at a voltage of 1.6 V (more than 200 h), show that the proposed biopolymer membrane could be an interesting alternative among the more environmentally friendly energy storage devices, while additionally it could be more economically justified.
Study on the Equation of State and Jet Forming of 3D-Printed PLA and PLA-Cu Materials
Authors: Yi Jianya , Hao Ruijie , Ji Qing , Guan Siman , Wang Zhijun , Yin Jianping
Published: August 2023

In order to improve the research and development efficiency and quality of low-density liners in production and scientific research development, PLA and PLA-Cu composite liners were prepared based on 3D-printing technology. In this paper, the relationship between the shock wave velocity D and the particle velocity u of PLA and PLA-Cu materials was tested by a one-stage light gas gun experiment device, and then the Grüneisen equation of state parameters of the two materials was obtained by fitting. The forming process of the two jets was numerically simulated by using the equation of state. When combined with the pulsed X-ray shooting results of the jets, it was found that the jets of the two materials showed obvious characteristics of “expansion particle flow”, and the head of the PLA jet had a gasification phenomenon. The length of the PLA jet at 20 μs in the numerical simulation was 127.2 mm, and the average length of the PLA jet at 20 μs in the pulsed X-ray shooting experiment was 100.45 mm. The length of the PLA jet gasification part accounted for about 21% of the total length of the jet. The average velocity of the head of the PLA jet is 7798.35 m/s, and the average velocity of the head of the PLA-Cu jet is 8104.25 m/s. In this paper, 3D-printing technology is used to prepare the liner for the first time, aiming to open up a new preparation technology and provide a new material selection for low-density material liners.
Investigating the Mechanism of Continuous–Discrete Coupled Destabilization of Roadway-Surrounding Rocks in Weakly Cemented Strata under Varying Levels of Moisture Content
Authors: Sun Lihui , Jiang Zhixin , Long Yaxin , He Qingfeng , Zhang Haiyang
Published: August 2023

This study examines frequent disasters, including large-scale deformation and collapse, caused by underground mining in weakly cemented strata in Western China. The weakly cemented rock’s unique characteristics, including low strength and easy disintegration, demonstrate a different damage pattern than that traditionally seen in the central and eastern regions. Using Fast Lagrangian Analysis of Continua-Particle Flow Code (FLAC2D-PFC2D) coupling, we model the strata, focusing on the 3-1 coal seam roadway at Hongqinghe mine. This study investigates the damage–rupture–destabilization progression in the peripheral rock under varying levels of moisture content. Our findings indicate that a water content of ω = 5.5% is the threshold for roadway damage, and moisture content <5.5% yields minimal rock deformation. However, moisture content >5.5% abruptly increases cracks and shifts the rock’s force chain, causing significant deformation and affecting the ceiling the most. Moreover, higher levels of moisture content weaken the anchor solid’s performance, with two primary failure modes: anchor interface slippage (comprising five stages: elasticity, elasticity–shear hardening, elasticity–shear hardening–decohesion, shear hardening–decohesion, and decohesion) and shear damage. These insights are vital for improving numerical simulations of underground mining, obtaining a more accurate understanding of mineral pressure disasters in weakly cemented strata mining regions in Western China, and developing a solid foundation for the better control of such strata.
Anti-Inflammatory and Antinociceptive Activity of Herbal Lipospheres of Pentaclethra macrophylla (Fabaceae) Stem Bark Extract
Authors: Nnamani Petra Obioma , Diovu Edith Obioma , Peter Ikechukwu Emmanuel , Onwuka Akachukwu Marytheresa , Ogbuanwu Chiamaka Victory , Abonyi Obiora Emmanuel , Loretz Brigitta , Lehr Claus-Michael
Published: August 2023

Purpose: Inflammation of various degrees is common among humans. There are associated side effects with orthodox delivery systems and anti-inflammatory agents; hence, the study investigated the characteristics of herbal lipospheres and the anti-inflammatory potency of the lipospheres formulated from Pentaclethra macrophylla with the view to having a drug with a better delivery system and lesser side effects. Methods: Herbal lipospheres were formulated using solidified reverse micellar solutions (SRMS) of P90H and goat fat and characterized for particle size and morphology, pH time dependent analysis, encapsulation efficiency (EE%), and Fourier Transform infrared spectroscopy. The in vitro antinociceptive and anti-inflammatory studies were carried out using membrane stabilization by hypotonicity-induced hemolysis and the determination of anti-platelet aggregatory activity models. The in vivo antinociceptive and anti-inflammatory studies on egg albumin- and formaldehyde-induced arthritis models were conducted. A total white blood cell count and differential blood count were carried out on the rats. Results: The results showed that there was no change in pH for the PM-unloaded lipospheres and 2.5 g of PM-loaded lipospheres from day 1 to day 7, but there was a mild variation in the rest of the formulations. The EE ranged from 35.2% to 94%, increasing according to the drug concentration. The photomicrographs of the lipospheres showed that the particles were spherical in shape. The particle sizes were within the acceptable range for lipospheres. FTIR showed no interaction. In the arthritis study, PM-loaded lipospheres inhibited edema consistently throughout the duration of observation. Inhibition of the membrane increased steadily with an increase in concentration of PM in the lipospheres and the standard drug. The platelet aggregatory inhibition decreased steadily with an increase in concentration of the PM in the lipospheres as well as the standard. The T50 dose of PM had the highest percentage of WBC, and it decreased as the treatment doses increased from T100 to T200. There were no significant differences among the Neutrophil counts of the different groups. Conclusions: The study, therefore, showed that the methanol extract of Pentaclethra macrophylla formed efficient herbal lipospheres with antinociceptive and anti-inflammatory activities.
Screening of Microbial Strains Used to Ferment Dendrobium officinale to Produce Polysaccharides, and Investigation of These Polysaccharides’ Skin Care Effects
Authors: Tang Xin , Wang Bulei , Mao Bingyong , Zhao Jianxin , Liu Guangrong , Yang Kaiye , Cui Shumao
Published: August 2023

The microbial fermentation of plants is a promising approach for enhancing the yield of polysaccharides with increased activity. In this study, ten microbial strains, Lactiplantibacillus plantarum CCFM8661, Limosilactobacillus reuteri CCFM8631, Lactobacillus helveticus M10, Lacticaseibacillus rhamnosus CCFM237, Lactilactobacillus sakei GD17-9, Lacticaseibacillus casei CCFM1073, Bacillus subtilis CCFM1162, Bacteroides cellulosilyticus FTJSI-E-2, Bacteroides stercoris FNMHLBEIK-4, and Saccharomyces cerevisiae HN7-A5, were used to ferment Dendrobium officinale. The skin care activity of the resulting polysaccharides (F-DOP) was evaluated in cultured HaCaT and RAW 264.7 cells, and a mouse model. The results indicated that D. officinale medium promoted strain proliferation, and fermentation significantly enhanced polysaccharide yield (up to 1.42 g/L) compared to that without fermentation (0.76 g/L). Moreover, F-DOPs, especially after CCFM8631 fermentation, exhibited an excellent ability to attenuate sodium dodecyl sulfate-induced HaCaT cell injury (from 69.04 to 94.86%) and decrease nitric oxide secretion (from 42.86 to 22.56 μM) in lipopolysaccharide-stimulated RAW 264.7 cells. In vivo, CCFM8631-FDOP reduced the transdermal water loss rate, skin epidermal thickness, and interleukin 6, and enhanced the expression of filaggrin, improving 2,4-dinitrofluorobenzene-induced skin damage. Therefore, considering viable cell counts, polysaccharide yields, and skin care efficacy in vitro and in vivo, CCFM8631 is the most suitable strain to enhance the skin care activity of DOPs and possesses promising potential for applications in the cosmetics industry.
Oscillatory Rheology of Three-Phase Coal Froths: Effects of Ionic Strength
Authors: Zhang Na , Kou Jue , Sun Chunbao , Zhu Yangge
Published: August 2023

The rheologic properties of a three-phase coal froth are critical to understanding the interfacial properties that are associated with its stability. Due to the fragile nature of froth, oscillatory rheology was used to make sure that the froths were not damaged during measurement. To reveal the relationship between a coal froth’s rheology and its stability, oscillatory rheology was used in this study. The viscoelastic behaviors of coal froths were analyzed, which illustrated that the storage modulus (G′) of a coal froth is larger than its loss modulus (G″), showing that coal froth is solid-like. The complex viscosity of the coal froths decreased with an increase in angular frequency, meaning that coal froth is shear-thinning. The dependence of froth rheology on ionic strength was investigated, which showed that an increase in ionic strength led to an enhancement of the storage modulus G′, as well as a decrease in tanδ (G″/G′). The coal froths tended to be more rigid and viscous with an increase in ionic strength. The mechanism of the effect of ionic strength on froth rheology was explored using electrical double layers, cryo-SEM, and particle fractions. As the ionic strength increased, the thickness of the electrical double layer decreased, which strengthened the interaction between the particles in the froth; in addition, the solid fraction in the froth increased with an increase in the ionic strength, so the value of G′ and the froth’s stability both increased.
Technological Advancements in Food Processing and Packaging
Authors: Fadiji Tobi , Pathare Pankaj B.
Published: August 2023

The global challenge of providing sufficient, safe, and sustainable food to a growing population requires continuous food processing and packaging technology advancements [...]
A Modelling-Based Framework for Carbon Emissions Calculation in Additive Manufacturing: A Stereolithography Case Study
Authors: Panagiotopoulou Vasiliki C. , Paraskevopoulou Aikaterini , Stavropoulos Panagiotis
Published: August 2023

Manufacturing is one of the most heavily contributing sectors to global warming via its high carbon emissions. Initiatives such as the Green Deal and Sustainable Goals by the United Nations are supporting the reduction of carbon emissions in the manufacturing sector, which can be completed by making manufacturing processes more sustainable and with less carbon footprint. This also applies to novel manufacturing processes such as additive manufacturing (AM). In this work, a previously developed framework for carbon footprint calculation was tailor-made and applied to a specific stereolithography (SLA) case. The different steps of the SLA were categorised per process, machine tool and system level, and the respective carbon emissions were calculated, either theoretically or via a life cycle assessment software. The carbon emissions at the process level were significant when compared to the total carbon emissions, and the carbon emissions of the isopropanol (IPA) bath accounted for more than 50% of the total carbon footprint of the SLA. These results demonstrate that the AM process may not be as environmentally friendly as it was assumed to be, especially if post-processing and finishing steps are carbon-intensive, because of the liquids used for the baths.
A Volume Fracturing Percolation Model for Tight Reservoir Vertical Wells
Authors: Du Dianfa , Liu Peng , Ren Lichuan , Li Yuan , Tang Yujie , Hao Fanghui
Published: August 2023

Based on the non-linear seepage characteristics of tight reservoirs and the reconstruction mode of vertical wells with actual volume fracturing, a seven-area percolation model for volume fracturing vertical wells in tight reservoirs is established. Laplace transform and Pedrosa transform are applied to obtain analytical solutions of bottom hole pressure and vertical well production under a constant production regime. After verifying the correctness of the model, the influence of the fracture network parameters on the pressure and production is studied. The research results indicate that as the permeability modulus increases, the production of volume fracturing vertical wells decreases. The penetration ratio of the main crack and the half-length of the main crack have a small impact on production, while the diversion capacity of the main crack has a significant impact on the initial production, but it is ultimately limited by the effective volume of the transformation. Under constant pressure conditions, the greater the width and permeability of the ESRV region, the higher the vertical well production rate is. The smaller the aspect ratio of the ESRV region, the higher the mid-term yield and the faster the yield decrease. The research results show guiding significance for the design of vertical well volume fracturing in tight reservoirs.