bluuebunny/crossref_metadata_2025 huggingface dataset:
Cardiovascular autonomic and peripheral sensory neuropathy in women with obesity
Authors: Keller Nóra , Zádori János , Lippai Balázs , Szöllősi Dalma , Márton Virág , Wellinger Károly , Lada Szilvia , Szűcs Mónika , Menyhárt Adrienn , Kempler Péter , Baczkó István , Várkonyi Tamás , Lengyel Csaba , Vágvölgyi Anna
Published: July 2024

Introduction

A higher incidence of neural dysfunction in people with obesity has been described. We determined the prevalence of neuropathic lesions in obese women and evaluated their potential association with anthropometric and laboratory parameters.

Patients and methods

In our cross-sectional study, we enrolled female patients with obesity and without diabetes before obesity treatment. Voluntary female subjects were controls with a normal body mass index (BMI). Autonomic function was assessed by Ewing’s cardiovascular reflex tests, while comprehensive peripheral neuropathic assessments were conducted utilizing the Neurometer®, Tiptherm®, Monofilament®, and Rydel-Seiffer tuning fork tests. Sudomotor function was assessed by the Neuropad®-test. Body composition was examined using the InBody 770.

Results

71 patients (mean ± SD; age: 36.1 ± 8.3 years; BMI: 40.2 ± 8.5 kg/m2) and 36 controls (age: 36.4 ± 13.3 years; BMI: 21.6 ± 2.1 kg/m2) were enrolled. Patients had significantly higher systolic (patients vs. controls; 137.5 ± 16.9 vs. 114.6 ± 14.8 mmHg, p<0.001) and diastolic (83.0 ± 11.7 vs.69.8 ± 11.2 mmHg, p<0.001) blood pressure compared to controls. Among autonomic tests, only the heart rate response to Valsalva maneuver (Valsalva-ratio) revealed significant impairment in patients (1.4 ± 0.2 vs. 1.7 ± 0.4, p<0.001). Neurometer® at the median nerve revealed increased current perception threshold (CPT) values at all stimulating frequencies in patients (CPT at 2000 Hz: 204.6 ± 70.9 vs. 168.1 ± 66.9, p=0.013; 250 Hz: 84.4 ± 38.9 vs. 56.5 ± 34.8, p<0.001; CPT at 5 Hz: 58.5 ± 31.2 vs 36.9 ± 29.1, p<0.001). The Rydel-Seiffer tuning fork test has revealed a significant impairment of vibrational sensing on the lower limb in patients (right hallux: 6.8 ± 0.9 vs. 7.4 ± 0.8, p=0.030; left hallux: 6.9 ± 0.8 vs. 7.3 ± 0.9, p=0.029). The Neuropad® testing showed a significant impairment of sudomotor function in women with obesity. A negative correlation was found in patients between BMI and the 25-hydroxy-D3/D2-vitamin levels (r=-0.41, p=0.00126) and a positive correlation between the BMI and resting systolic blood pressure (r=0.26, p=0.0325).

Conclusion

Peripheral sensory neuronal and sudomotor function impairments were detected in female patients with obesity compared to the controls with normal BMI. Cardiovascular autonomic dysfunction was also revealed by the Valsalva-ratio in these patients, suggesting the presence of parasympathetic dysfunction. The negative correlation between BMI and the 25-hydroxy-D3/D2-vitamin highlights the potential deficiency of vitamin D in the population affected by obesity.
Thyroid dysfunction in the wake of Omicron: understanding its role in COVID-19 severity and mortality
Authors: Zhang Qingfeng , Zhang Zongyue , Liu Xu , Wang Yixuan , Chen Hao , Hao Yueying , Zha Shiqian , Zhang Jingyi , He Yang , Zhou Beini , Hu Ke
Published: July 2024

Purpose

SARS-CoV-2 can invade the thyroid gland. This study was to delineate the risk of thyroid dysfunction amidst the prevalence of the Omicron variant, and to investigate the correlation between thyroid function and Coronavirus disease 2019 (COVID-19) outcomes. The study also aimed to ascertain whether thyroid dysfunction persisted during COVID-19 recovery phase.

Methods

This was a retrospective cohort study. COVID-19 patients from the Renmin Hospital of Wuhan University, China during the epidemic of Omicron variants were included, and their thyroid function were analyzed in groups.

Results

A history of thyroid disease was not associated with COVID-19 outcomes. COVID-19 can lead to a bimodal distribution of thyroid dysfunction. The severity of COVID-19 was inversely proportional to the levels of thyroid- stimulating hormone (TSH), free triiodothyronine (FT3) and free thyroxine (FT4), leading to a higher prevalence of thyroid dysfunction. Severe COVID-19 was a risk factor for euthyroid sick syndrome (ESS) (OR=22.5, 95% CI, 12.1 - 45.6). Neutrophil to lymphocyte ratio mediated the association between severe COVID-19 and ESS (mediation effect ratio = 41.3%, p < 0.001). ESS and decreased indicators of thyroid function were associated with COVID-19 mortality, while high levels of FT3 and FT4 exhibited a protective effect against death. This effect was more significant in women (p < 0.05). During the recovery period, hyperthyroidism was quite uncommon, while a small percentage of individuals (7.7%) continued to exhibit hypothyroidism.

Conclusion

COVID-19 severity was linked to thyroid dysfunction. Severe COVID-19 increased the risk of ESS, which was associated with COVID-19 mortality. Post-recovery, hyperthyroidism was rare, but some individuals continued to have hypothyroidism.
Synergic effect of thermo-chemical pretreatment of waste-activated sludge on bio-methane enhancement
Authors: Almegbl A. M. , Munshi F. , Khursheed A.
Published: July 2024

Sustainable and environmentally friendly energy production is feasible via anaerobic digestion (AD) of organic wastes, such as waste-activated sludge (WAS). However, due to its limited degradation, a pretreatment strategy is applied to WAS to enhance its bio-degradation and, thus, biogas yield. Alkaline (0.5%–9% g NaOH/gTS, 30 min), microwave (MW) (90°C–175°C), and hybrid (0.5% g NaOH/gTS +125°C) pretreatments were applied to WAS. The characterization of untreated and pretreated WAS revealed that with higher alkaline and MW pretreatment, the soluble chemical oxygen demand (sCOD), carbohydrate, and protein increased; however, the readily biodegradable COD (rbCOD) rate was unlike the sCOD. The sCOD was 7%–18%, 8%–23%, and 37% for alkaline, MW, and hybrid pretreatments, respectively. Stronger alkaline and MW pretreatment induced higher turbidity, capillary suction time, and lower average particle size. AD of alkaline-, MW-, and hybrid-pretreated WAS produced 94% (0.5% NaOH), 125% (MW at 125°C), and 199% (0.5% NaOH and MW at 125°C) increased biogas, respectively, compared to the AD of untreated sludge. The AD data on the alkaline-, MW-, and hybrid-pretreated BMP assays fitted well with the modified Gompertz model with a coefficient of determination above 0.95. The PCA analysis showed that biogas production is closely correlated with pretreatment temperature, VFA production, rbCOD, sCOD, and soluble carbohydrates and protein. Microbial genome sequencing analysis showed an improvement in microbial abundance and diversity. Acetoclastic methanogen (Methanothrix) growth was improved by 37% (MW pretreatment). Abundances of Methanosarcina, using all three metabolic pathways for methanogenesis, were 17, 21, 11, and 48% in the control, alkaline-, MW-, and hybrid-pretreated digestate, respectively, corresponding to 186% improvement in hybrid pretreatment when compared to the control.
Land degradation neutrality and carbon neutrality: approaches, synergies, and challenges
Authors: Gunawardena M. Amritha , Lokupitiya Erandathie , Gunawardena Prasanthi
Published: July 2024

Land is being degraded rapidly worldwide. United Nations Convention to Combat Desertification in 2015 has invited countries to formulate voluntary targets to achieve Land Degradation Neutrality (LDN). Under the Paris Agreement, a legally binding international treaty adopted in 2015, the world is transitioning toward Carbon Neutrality (CN) with more mitigation actions. This paper intended to review the concepts of land degradation, LDN along with CN emphasizing the degradation types, approaches, models available to analyze, synergies, economic aspects and challenges. The review explores approaches and models available for achieving LDN and CN which are both synergistic, economically efficient and could overcome the common challenges. Land degradation has to focus beyond the traditional definitions to incorporate more persistent and the difficult to restore degradation causes. Such complex land degradation requires specialized LDN approaches. The level of degradation and restoration progress could be analyzed using a variety of modeling approaches including economic models. Approaches for LDN and CN can bring significant synergies for each other. The approach proposed by the present study will provide a logical flow for decision-making while minimizing time and effort and avoiding a piecemeal approach. The approach therefore maximizes the output in relation to the inputs thus enhancing sustainability.
Single-cell mass cytometry in immunological skin diseases
Authors: Zhao Mingming , Cheng Yuqi , Gao Jinping , Zhou Fusheng
Published: July 2024

Immune-related skin diseases represent a collective of dermatological disorders intricately linked to dysfunctional immune system processes. These conditions are primarily characterized by an immoderate activation of the immune system or deviant immune responses, involving diverse immune components including immune cells, antibodies, and inflammatory mediators. However, the precise molecular dysregulation underlying numerous individual cases of these diseases and unique subsets respond under disease conditions remains elusive. Comprehending the mechanisms and determinants governing the homeostasis and functionality of diseases could offer potential therapeutic opportunities for intervention. Mass cytometry enables precise and high-throughput quantitative measurement of proteins within individual cells by utilizing antibodies labeled with rare heavy metal isotopes. Imaging mass cytometry employs mass spectrometry to obtain spatial information on cell-to-cell interactions within tissue sections, simultaneously utilizing more than 40 markers. The application of single-cell mass cytometry presents a unique opportunity to conduct highly multiplexed analysis at the single-cell level, thereby revolutionizing our understanding of cell population heterogeneity and hierarchy, cellular states, multiplexed signaling pathways, proteolysis products, and mRNA transcripts specifically in the context of many autoimmune diseases. This information holds the potential to offer novel approaches for the diagnosis, prognostic assessment, and monitoring responses to treatment, thereby enriching our strategies in managing the respective conditions. This review summarizes the present-day utilization of single-cell mass cytometry in studying immune-related skin diseases, highlighting its advantages and limitations. This technique will become increasingly prevalent in conducting extensive investigations into these disorders, ultimately yielding significant contributions to their accurate diagnosis and efficacious therapeutic interventions.
Enhancing acute inflammatory and sepsis treatment: superiority of membrane receptor blockade
Authors: Mun Seok-Jun , Cho Euni , Kim Hyo Keun , Gil Woo Jin , Yang Chul-Su
Published: July 2024

Conditions such as acute pancreatitis, ulcerative colitis, delayed graft function and infections caused by a variety of microorganisms, including gram-positive and gram-negative organisms, increase the risk of sepsis and therefore mortality. Immune dysfunction is a characterization of sepsis, so timely and effective treatment strategies are needed. The conventional approaches, such as antibiotic-based treatments, face challenges such as antibiotic resistance, and cytokine-based treatments have shown limited efficacy. To address these limitations, a novel approach focusing on membrane receptors, the initiators of the inflammatory cascade, is proposed. Membrane receptors such as Toll-like receptors, interleukin-1 receptor, endothelial protein C receptor, μ-opioid receptor, triggering receptor expressed on myeloid cells 1, and G-protein coupled receptors play pivotal roles in the inflammatory response, offering opportunities for rapid regulation. Various membrane receptor blockade strategies have demonstrated efficacy in both preclinical and clinical studies. These membrane receptor blockades act as early stage inflammation modulators, providing faster responses compared to conventional therapies. Importantly, these blockers exhibit immunomodulatory capabilities without inducing complete immunosuppression. Finally, this review underscores the critical need for early intervention in acute inflammatory and infectious diseases, particularly those posing a risk of progressing to sepsis. And, exploring membrane receptor blockade as an adjunctive treatment for acute inflammatory and infectious diseases presents a promising avenue. These novel approaches, when combined with antibiotics, have the potential to enhance patient outcomes, particularly in conditions prone to sepsis, while minimizing risks associated with antibiotic resistance and immune suppression.
Tradition unveiled: a comprehensive review of microbiological studies on Portuguese traditional cheeses, merging conventional and OMICs analyses
Authors: Serrano Susana , Morais Susana , Semedo-Lemsaddek Teresa
Published: July 2024

The microbial communities inhabiting Portuguese traditional cheeses play a fundamental role in shaping their unique flavor, texture, and safety characteristics. This comprehensive review synthesizes findings from both conventional microbiological studies and advanced OMICs analyses to provide a deeper understanding of the microbiota dynamics in these cheeses. We explore the microbial composition, diversity, and functional roles of bacteria, yeasts, and molds across various Protected Designation of Origin (PDO) cheeses, highlighting their contributions to cheese ripening, flavor development, and safety. Additionally, we discuss the potential of OMICs technologies, namely metagenomics, in unraveling the complex microbial ecosystems of Portuguese traditional cheeses. Through this integrative approach, we aim to shed light on the intricate interplay between microorganisms and cheese matrices, unveiling the secrets behind the rich heritage and distinctiveness of Portuguese traditional cheeses.
Predicting malaria hyper endemic zones in West Africa using a regional scale dynamical malaria model
Authors: Olaniyan Eniola A. , Tompkins Adrian M. , Caminade Cyril
Published: July 2024

Due to the continuing large number of malaria-related deaths in tropical Africa, the need to develop a robust Malaria Early Warning System (MEWS) for effective action is growing to guide cost-effective implementation of interventions. This study employs a two-stage hierarchical evaluation technique to evaluate the ability of the VECTRI malaria model to simulate malaria dynamics at seasonal time scale (1 - 7 months) over Nigeria and West Africa. Two sets of malaria simulations are considered. The first set is based on VECTRI simulations driven by observed rainfall and temperature datasets (hereafter referred to as control run). The second is based on malaria simulations driven by the European Centre for Medium-Range Weather Forecasting (ECMWF) System5 ensemble seasonal forecasting system (hereafter referred to as Forecast run). Different metrics are employed to assess the skill of the VECTRI malaria model. Results based on the control run indicate that the model can reproduce hyper-endemic zones and the evolution of malaria cases, particularly the observed increase in cases with decreasing population density. Despite having significant biases and low correlation, the model successfully predicts annual anomalies in malaria cases across Nigeria, particularly in the savannah region that experience large malaria burden. Annual correlations between the VECTRI Forecast run and the VECTRI Control run are relatively low at all lead times (LT) and for each start date (SD) across West Africa, although correlation generally increases from the Gulf of Guinea to the Sahel. Despite low correlations, the Rank Probability Skill Score (RPSS) reveals that the model has a statistically significant skill in predicting malaria occurrences across all categories of malaria cases, regardless of start date or lead time. While the Guinea Forest has the strongest RPSS, the increase or decrease in skill from the first to seventh lead time varies significantly across the region. In addition, the VECTRI malaria model has a good ability to discriminate variability in malaria cases across all regions, with an average Area Under the Relative Operating Characteristics (ROC) Curve (AUC) of approximately 0.62. Our findings suggest that the VECTRI malaria model could be used as a reliable Malaria Early Warning System (MEWS), particularly for identifying malaria hyper-endemic zones in West Africa at seasonal time scale.
Langerhans cell histiocytosis: unusual bone marrow infiltration—report of 2 cases in Ecuador
Authors: Santana Paulina , Arias-Intriago Marlon , Izquierdo-Condoy Juan S.
Published: July 2024

Langerhans cell histiocytosis (LCH) is a histiocytic neoplasm characterized by the abnormal proliferation of Langerhans cells. Bone marrow (BM) involvement is associated with high-risk disease and poor survival. Although BM involvement is particularly uncommon, no reported cases of LCH with BM infiltration have been documented in Latin America until now. The aim of this report is to highlight the clinical, hematological, and BM findings of two cases of LCH with BM infiltration, providing insights that may aid in detecting suspected patients. We present two cases of LCH with BM infiltration. One case involved a 23-month-old male patient, and the other a 16-month-old female patient. Common clinical findings in both cases included hepatosplenomegaly and fever. Hematological findings revealed anemia in both cases. The key diagnostic tool was the BM biopsy, which revealed histiocyte nests with characteristic morphology, CD1a-positive cells, increased eosinophils, and reactive paratrabecular lymphocytes. This report underscores the significance of clinical profiles in predicting BM infiltration in LCH. The presence of histiocyte nests displaying the characteristic morphology of Langerhans cells, accompanied by an elevation in eosinophils, indicates bone marrow involvement. Furthermore, the demonstration of CD1a-positive cells through immunohistochemistry serves as a crucial diagnostic tool.
Respiratory diseases and gut microbiota: relevance, pathogenesis, and treatment
Authors: Sun Mengdi , Lu Fang , Yu Donghua , Wang Yu , Chen Pingping , Liu Shumin
Published: July 2024

Preclinical evidence has firmly established a bidirectional interaction among the lung, gut, and gut microbiome. There are many complex communication pathways between the lung and intestine, which affect each other's balance. Some metabolites produced by intestinal microorganisms, intestinal immune cells, and immune factors enter lung tissue through blood circulation and participate in lung immune function. Altered gut–lung–microbiome interactions have been identified in rodent models and humans of several lung diseases such as pulmonary fibrosis, chronic obstructive pulmonary disease, lung cancer, asthma, etc. Emerging evidence suggests that microbial therapies can prevent and treat respiratory diseases, but it is unclear whether this association is a simple correlation with the pathological mechanisms of the disease or the result of causation. In this review, we summarize the complex and critical link between the gut microbiota and the lung, as well as the influence and mechanism of the gut microbiota on respiratory diseases, and discuss the role of interventions such as prebiotics and fecal bacteria transplantation on respiratory diseases. To provide a reference for the rational design of large-scale clinical studies, the direct application of microbial therapy to respiratory-related diseases can reduce the incidence and severity of diseases and accompanying complications.
The infants’ gut microbiome: setting the stage for the early onset of obesity
Authors: Vallès Yvonne , Arshad Muhammad , Abdalbaqi Mamoun , Inman Claire K. , Ahmad Amar , Drou Nizar , Gunsalus Kristin C. , Ali Raghib , Tahlak Muna , Abdulle Abdishakur
Published: July 2024

In the past three decades, dietary and lifestyle changes worldwide have resulted in a global increase in the prevalence of obesity in both adults and children. Known to be highly influenced by genetic, environmental and lifestyle factors, obesity is characterized by a low-grade chronic inflammation that contributes to the development of other metabolic diseases such as diabetes and cardiovascular disease. Recently, the gut microbiome has been added as a cause/contributor to the development of obesity. As differences in the microbiome between obese and normoweight individuals have been observed, we set out to determine whether infants harbor an obesogenic microbiome early on and whether the pre-pregnancy status of the mother (obese or normoweight) is correlated to their infant’s microbiome composition. Using shotgun sequencing, we analyzed stool samples throughout the first year of life from infants born to obese (n = 23 participants, m = 104 samples) and normoweight (n = 23 participants, m = 99 samples) mothers. We found that the infants’ microbiome diversity at taxonomic and functional levels was significantly influenced by time (ANOVA p < 0.001) but not by the mother’s pre-pregnancy status. Overall, no deterministic succession of taxa or functions was observed. However, infants born to obese mothers were found to have a significantly higher Bacillota/Bacteroidota ratio (p = 0.02) at six months, were significantly depleted from six months old of the well-established obesity biomarkers Akkermansia municiphila and Faecalibacterium prausnitzii (p < 0.01), and were at one week old, significantly enriched in pathways such as the UDP-N-acetyl-D-glucosamine biosynthesis II (p = 0.02) involved in leptin production, suggesting perhaps that there may exist some underlying mechanisms that dictate the development of an obesogenic microbiota early on.
The functional identification and evaluation of endophytic bacteria sourced from the roots of tolerant Achyranthes bidentata to overcome monoculture problems of Rehmannia glutinosa
Authors: Zeng Chunli , Liu Yazhou , Zhang Bianhong , Zhang Chenjing , Li Niu , Ji Leshan , Lan Chaojie , Qin Bin , Yang Yuncheng , Wang Juanying , Chen Ting , Fang Changxun , Lin Wenxiong
Published: July 2024

The isolation and identification of plant growth-promoting endophytic bacteria (PGPEB) from Achyranthes bidentata roots have profound theoretical and practical implications in ecological agriculture, particularly as bio-inoculants to address challenges associated with continuous monoculture. Our research revealed a significant increase in the abundance of these beneficial bacteria in A. bidentata rhizosphere soil under prolonged monoculture conditions, as shown by bioinformatics analysis. Subsequently, we isolated 563 strains of endophytic bacteria from A. bidentata roots. Functional characterization highlighted diverse plant growth-promoting traits among these bacteria, including the secretion of indole-3-acetic acid (IAA) ranging from 68.01 to 73.25 mg/L, phosphorus and potassium solubilization capacities, and antagonistic activity against pathogenic fungi (21.54%−50.81%). Through 16S rDNA sequencing, we identified nine strains exhibiting biocontrol and growth-promoting potential. Introduction of a synthetic microbial consortium (SMC) in pot experiments significantly increased root biomass by 48.19% in A. bidentata and 27.01% in replanted Rehmannia glutinosa. These findings provide innovative insights and strategies for addressing continuous cropping challenges, highlighting the practical promise of PGPEB from A. bidentata in ecological agriculture to overcome replanting obstacles for non-host plants like R. glutinosa, thereby promoting robust growth in medicinal plants.
Age-correlated changes in the canine oral microbiome
Authors: Kislik Gregory , Zhou Lin , Rubbi Liudmilla , Pellegrini Matteo
Published: July 2024

Introduction

Canine oral disease has been associated with significant changes in the oral microbiome rather than the presence or absence of individual species. In addition, most studies focus on a single age group of canines and as of yet, the relationship between canine microbiomes and age is poorly understood.

Methods

This study used a shotgun whole gene sequencing approach in tandem with the Aladdin Bioinformatics platform to profile the microbiomes of 96 companion dogs, with the sourmash-zymo reference database being used to perform taxonomic profiling.

Results

Findings showed significant age correlations among 19 species, including positive correlations among several Porphyromonas species and a negative correlation with C. steedae. Although a significant correlation was found between predicted and actual ages, ElasticNet Regression was unable to successfully predict the ages of younger canines based on their microbiome composition. Both microbiome samples and microbial species were successfully clustered by age group or age correlation, showing that the age-microbiome relationship survives dimensionality reduction. Three distinct clusters of microbial species were found, which were characterized by Porphyromonas, Conchiformibius, and Prevotella genera, respectively.

Discussion

Findings showed that the microbiomes of older dogs resembled those that previous literature attributed to dogs with periodontal disease. This suggests that the process of aging may introduce greater risks for canine oral disease.
Robust anti-tubercular profile of Solanum virginianum extract in enhancing isoniazid bioavailability and curtailing stress tolerance in Mycobacterium smegmatis
Authors: Balkrishna Acharya , Joshi Monali , Kabdwal Manisha , Tomer Meenu , Lochab Savita , Varshney Anurag
Published: July 2024

Introduction

The formidable survival mechanisms employed by Mycobacterium tuberculosis (Mtb), combined with the low bioavailability of anti-tubercular drugs and their associated hepatotoxicity, worsen tuberculosis management. Traditional medicinal plants offer potential solutions to these challenges. This study focuses on exploring the anti-tubercular potential of Solanum virginianum against Mycobacterium smegmatis, mc2155.

Methods and results

HPTLC and UHPLC phytochemically characterized the hydro-methanolic extract of Solanum virginianum (SVE). SVE curtails the growth and viability of mc2155 under normal and in vitro stress conditions. The compromised cell wall integrity of mc2155 with SVE is depicted through scanning electron microscopy (SEM) while EtBr permeability assays and TLC-based comparative changes in lipids extraction addressed the integrity of the cell wall. Furthermore, SVE augmented the susceptibility of mc2155 towards Isoniazid (INH) through enhanced bioavailability. Adjunct treatment of SVE with INH demonstrated a markedly reduced survival of the intracellular bacilli. The study also uncovered the hepatoprotective potential of SVE in HepG2 cells.

Conclusion

This research paves the way for deeper exploration into the potential of Solanum virginianum against virulent Mtb strains, emphasizing over the significance of traditional medicinal plants in tuberculosis treatment. Collectively, the findings suggest SVE as a potent candidate for independent or adjunct anti-tubercular therapy.
Biochar, microbes, and biochar-microbe synergistic treatment of chlorinated hydrocarbons in groundwater: a review
Authors: Niu Shixin , Li Changsuo , Gao Shuai , Tian Jingya , Zhang Chao , Li Lixia , Huang Yao , Lyu Honghong
Published: July 2024

Dehalogenating bacteria are still deficient when targeted to deal with chlorinated hydrocarbons (CHCs) contamination: e.g., slow metabolic rates, limited substrate range, formation of toxic intermediates. To enhance its dechlorination capacity, biochar and its composites with appropriate surface activity and biocompatibility are selected for coupled dechlorination. Because of its special surface physical and chemical properties, it promotes biofilm formation by dehalogenating bacteria on its surface and improves the living environment for dehalogenating bacteria. Next, biochar and its composites provide active sites for the removal of CHCs through adsorption, activation and catalysis. These sites can be specific metal centers, functional groups or structural defects. Under microbial mediation, these sites can undergo activation and catalytic cycles, thereby increasing dechlorination efficiency. However, there is a lack of systematic understanding of the mechanisms of dechlorination in biogenic and abiogenic systems based on biochar. Therefore, this article comprehensively summarizes the recent research progress of biochar and its composites as a “Taiwan balm” for the degradation of CHCs in terms of adsorption, catalysis, improvement of microbial community structure and promotion of degradation and metabolism of CHCs. The removal efficiency, influencing factors and reaction mechanism of the degraded CHCs were also discussed. The following conclusions were drawn, in the pure biochar system, the CHCs are fixed to its surface by adsorption through chemical bonds on its surface; the biochar composite material relies on persistent free radicals and electron shuttle mechanisms to react with CHCs, disrupting their molecular structure and reducing them; biochar-coupled microorganisms reduce CHCs primarily by forming an “electron shuttle bridge” between biological and non-biological organisms. Finally, the experimental directions to be carried out in the future are suggested to explore the optimal solution to improve the treatment efficiency of CHCs in water.
Incidence and influencing factors of olfactory dysfunction in patients 1 week after endoscopic transsphenoidal resection of pituitary tumor: a cross-sectional study of 158 patients
Authors: Zhou Sumei , Zeng Zhi , Chen Min , Zou Linbo , Shao Shirong
Published: July 2024

Objective

To investigate the current situation of olfactory dysfunction in patients after endoscopic transsphenoidal resection of pituitary tumors, and analyze its influencing factors, to provide references for clinical nursing and rehabilitation.

Methods

A cross-sectional study design and convenience sampling method were used to investigate 158 patients with pituitary tumors treated by endoscopic transsphenoidal pituitary tumor resection in the Department of Neurosurgery of three Grade-A general hospitals in Sichuan Province from January 2022 and June 2023. The olfactory function of patients was evaluated 1 week after surgery, and the general clinical data and olfactory related data of patients were collected, and the influencing factors of olfactory disorder were analyzed by logistic regression.

Results

The incidence of olfactory dysfunction was 73.42%. analysis revealed that the formation of blood scabs, nasal cavity adhesion, cerebrospinal fluid leakage and operation time were independent risk factors for olfactory dysfunction in patients after transsphenoidal pituitary tumor resection (p < 0.05).

Conclusion

The incidence of olfactory dysfunction is high in patients after endoscopic transsphenoidal resection of pituitary tumors, suggesting that medical staff should pay close attention to and identify patients with olfactory dysfunction based on the guidance of disease knowledge and skills, develop targeted nursing interventions, and promote the improvement of patients’ olfactory function and quality of life.
A novel minimally invasive and versatile kyphoplasty balloon-based model of porcine spinal cord injury
Authors: Barber Sean M. , Wolfe Tatiana , Steele Alexander G. , Hoffman Kris , Hogan Matthew K. , Frazier Allison , Tang Xiufeng , Sayenko Dimitry G. , Horner Philip J.
Published: July 2024

Introduction

Spinal cord injury (SCI) animal models often utilize an open surgical laminectomy, which results in animal morbidity and also leads to changes in spinal canal diameter, spinal cord perfusion, cerebrospinal fluid flow dynamics, and spinal stability which may confound SCI research. Moreover, the use of open surgical laminectomy for injury creation lacks realism when considering human SCI scenarios.

Methods

We developed a novel, image-guided, minimally invasive, large animal model of SCI which utilizes a kyphoplasty balloon inserted into the epidural space via an interlaminar approach without the need for open surgery.

Results

The model was validated in 5 Yucatán pigs with imaging, neurofunctional, histologic, and electrophysiologic findings consistent with a mild compression injury.

Discussion

Few large animal models exist that have the potential to reproduce the mechanisms of spinal cord injury (SCI) commonly seen in humans, which in turn limits the relevance and applicability of SCI translational research. SCI research relies heavily on animal models, which typically involve an open surgical, dorsal laminectomy which is inherently invasive and may have untoward consequences on animal morbidity and spinal physiology that limit translational impact. We developed a minimally invasive, large animal model of spinal cord injury which utilizes a kyphoplasty balloon inserted percutaneously into the spinal epidural space. Balloon inflation results in a targeted, compressive spinal cord injury with histological and electrophysiological features directly relevant to human spinal cord injury cases without the need for invasive surgery. Balloon inflation pressure, length of time that balloon remains inflated, and speed of inflation may be modified to achieve variations in injury severity and subtype.
Alpha and theta oscillations on a visual strategic processing task in age-related hearing loss
Authors: Shende Shraddha A. , Jones Sarah E. , Mudar Raksha A.
Published: July 2024

Introduction

Emerging evidence suggests changes in several cognitive control processes in individuals with age-related hearing loss (ARHL). However, value-directed strategic processing, which involves selectively processing salient information based on high value, has been relatively unexplored in ARHL. Our previous work has shown behavioral changes in strategic processing in individuals with ARHL. The current study examined event-related alpha and theta oscillations linked to a visual, value-directed strategic processing task in 19 individuals with mild untreated ARHL and 17 normal hearing controls of comparable age and education.

Methods

Five unique word lists were presented where words were assigned high- or low-value based on the letter case, and electroencephalography (EEG) data was recorded during task performance.

Results

The main effect of the group was observed in early time periods. Specifically, greater theta synchronization was seen in the ARHL group relative to the control group. Interaction between group and value was observed at later time points, with greater theta synchronization for high- versus low-value information in those with ARHL.

Discussion

Our findings provide evidence for oscillatory changes tied to a visual task of value-directed strategic processing in individuals with mild untreated ARHL. This points towards modality-independent neurophysiological changes in cognitive control in individuals with mild degrees of ARHL and adds to the rapidly growing literature on the cognitive consequences of ARHL.
Association between different composite dietary antioxidant indexes and constipation in American male adults: a cross-sectional study
Authors: Sun Wei , Wang Yuchao , Han Lu , Liu Yinshi , Liu Hongru , Tong Yunbing , Jiang Ziying , Xu Chen , Sun Daqing
Published: July 2024

Background

Oxidative stress is acknowledged as a pivotal factor in the intricate pathophysiological processes and pathogenesis of constipation. Modifying dietary patterns can elevate in vivo antioxidant biomarker levels, consequently mitigating oxidative stress. The Composite Dietary Antioxidant Index (CDAI) provides a dependable scoring mechanism for quantifying the potential antioxidant capacity of diets. The association between CDAI levels and the risk of constipation remains uncertain.

Purpose

To investigate the potential correlation between CDAI and constipation, aiming to improve constipation management through dietary guidance.

Methods

A total of 11,165 adults aged ≥20 years, drawn from the 2005–2010 National Health and Nutrition Examination Survey, were enrolled in this cross-sectional study. We evaluated the correlation between CDAI levels and the risk of constipation through three weighted logistic regression models. Restricted cubic spline (RCS) analysis was employed to assess nonlinear trends, and stratified analyses were conducted.

Results

After adjusting for all confounding variables, the findings revealed an association between CDAI and constipation [OR = 0.937; 95% CI (0.892, 0.984), p = 0.012]. Moreover, individuals in the highest quartile of CDAI demonstrated a 40.1% lower likelihood of experiencing constipation compared to those in the lowest quartile [OR = 0.599; 95% CI (0.382, 0.939), p = 0.027]. The RCS analysis indicated a linear relationship between CDAI and constipation (P-non-linear =0.1016). Subgroup analysis by gender revealed a negative correlation in the male population [OR = 0.871; 95% CI (0.801, 0.947), p = 0.002], with men in the highest CDAI quartile exhibiting a 59.8% lower likelihood of experiencing constipation compared to those in the lowest quartile [OR = 0.402; 95% CI (0.206, 0.787), p = 0.010]. Furthermore, alterations in selenium [OR = 0.997; 95% CI (0.995, 1.000), p = 0.039] per milligram were independently linked to constipation. In a gender subgroup analysis of a single antioxidant, changes per milligram of vitamin E [OR = 0.904; 95% CI (0.838 to 0.975), p = 0.011] among males were independently associated with constipation.

Conclusion

The fully adjusted model showed a correlation between CDAI and constipation and a significant correlation in quartiles. Meanwhile, subgroup analysis by gender showed that CDAI was negatively associated with constipation in the male population. Moreover, the findings of this study imply that investigations into antioxidant diets should be contextualized within dietary patterns.
Case report: Possible role of low-dose PEM for avoiding unneeded procedures associated with false-positive or equivocal breast MRI results
Authors: Rapley Madeline , Freitas Vivianne , Weinberg Irving N. , Baldassi Brandon , Poladyan Harutyun , Waterston Michael , Ghai Sandeep , Taeb Samira , Bubon Oleksandr , Mulligan Anna Marie , Reznik Alla
Published: July 2024

Contrast-enhanced breast magnetic resonance imaging (MRI) is currently recommended as a screening tool for high-risk women and has been advocated for women with radiologically dense breast tissue. While breast MRI is acknowledged for its high sensitivity (with an exception for lower-grade ductal carcinoma in situ (DCIS) where emerging techniques like diffusion-weighted imaging offer improvement), its limitations include sensitivity to hormonal changes and a relatively high false-positive rate, potentially leading to overdiagnosis, increased imaging uncertainty, and unnecessary biopsies. These factors can exacerbate patient anxiety and impose additional costs. Molecular imaging with breast-targeted Positron Emission Tomography (PET) has shown the capability to detect malignancy independent of breast density and hormonal changes. Furthermore, breast-targeted PET has shown higher specificity when compared with MRI. However, traditional PET technology is associated with high radiation dose, which can limit its widespread use particularly in repeated studies or for undiagnosed patients. In this case report, we present a clinical application of low-dose breast imaging utilizing a breast-targeted PET camera (Radialis PET imager, Radialis Inc). The case involves a 33-year-old female patient who had multiple enhanced lesions detected on breast MRI after surgical removal of a malignant phyllodes tumor from the right breast. A benign core biopsy was obtained from the largest lesion seen in the left breast. One month after the MRI, 18F-fluorodeoxyglucose (18F-FDG) PET imaging session was performed using the Radialis PET Imager. Although the Radialis PET Imager has proven high count sensitivity and the capability to detect breast lesions with low metabolic activity (at a dose similar to mammography), no areas of increased 18F-FDG uptake were visualized in this particular case. The patient underwent a right-sided nipple-sparing mastectomy and left-sided lumpectomy, with bilateral reconstruction. The excised left breast tissue was completely benign, as suggested by both core biopsy and the PET results. The case presented highlights a promising clinical application of low-dose breast-targeted PET imaging to mitigate the uncertainty associated with MRI while keeping radiation doses within the safe range typically used in X-ray mammography.
The effects of thermalization on the performance of passively Q-switched IC-OPOs
Authors: Kneller Josh , Salimian Rizi Saeed , Flannigan Liam , Xu Chang-qing
Published: July 2024

In this paper, we report a new model for passively Q-switched intracavity optical parametric oscillators (IC-OPOs), using Cr:YAG as a Q-switch crystal. The model considers the effects of thermalization in the Nd:YAG laser crystal, excited state absorption in the Cr:YAG saturable absorber, nonlinear loss due to pump depletion in the nonlinear crystal, the thermal lens effect and the mode overlap of the fields within the nonlinear crystal. The calculated pulse width for the 1,064 nm pump light was 6.2 ns compared to the experimental result of 6 ns, while the signal wave was calculated to have a pulse width of 1.75 ns compared to the experimental result of 2 ns. The model is shown to accurately calculate both the temporal and output power characteristics of the IC-OPO, which has not been previously reported to the best of our knowledge.
Fusarium Tropical Race 4 in Latin America and the Caribbean: status and global research advances towards disease management
Authors: Munhoz Thayne , Vargas Jorge , Teixeira Luiz , Staver Charles , Dita Miguel
Published: July 2024

Fusarium wilt of banana (FWB), caused by the soil-borne fungus Fusarium oxysporum f. sp. cubense (Foc), poses an undeniable threat to global banana production. This disease has intensified in recent years, with the tropical race 4 (TR4) strain spreading rapidly. Since 2018, the number of affected countries has increased from 16 to 23, presenting a significant challenge to researchers, producers, and National Plant Protection Organizations (NPPOs) worldwide. The potential impact of TR4 in Latin America and the Caribbean (LAC) is particularly concerning. This region boasts seven of the top ten banana-exporting countries, and bananas and plantains are crucial for food security and income generation. In Colombia, where TR4 was detected in 2019, the disease has already spread from La Guajira to Magdalena, and it is currently affecting 20 large commercial export farms. In Peru, the disease was detected in 2021 and although still restricted to the northern region, flood irrigation and heavy rains associated with the Yaku cyclone, boosted pathogen spread, and more than 400 small organic banana farmers are currently affected. In Venezuela, TR4 detection occurred in 2023, with plantations across three states and five municipalities now affected. Worryingly, TR4 has also been confirmed in plantains, a staple food in the region. Current national responses in LAC primarily rely on preventive and reactive measures: preventing initial incursions and containing outbreaks to avoid further spread. However, the disease’s relentless progression suggests that its eventual presence in all banana-producing areas is likely. Therefore, exploring alternative management approaches beyond pathogen exclusion becomes crucial, both in affected and disease-free regions. This paper examines the current spread of TR4, focusing on epidemiological aspects and recent research-based management options. Key epidemiological features were highlighted, drawing practical examples from various scales (plots to landscapes) and utilizing experiences from LAC’s fight against TR4. The paper also reviews field-tested approaches in biosecurity, biological control, resistant varieties, soil health, and integrated disease management, acknowledging the specific challenges faced by smallholder settings. In each section research initiatives were analyzed, identifying gaps, and proposing directions to minimize TR4 impact and accelerate the development of sustainable solutions for managing this devastating disease.
Validation of the Maslach Burnout Inventory-General Survey 9-item short version: psychometric properties and measurement invariance across age, gender, and continent
Authors: Wang Anni , Duan Yinfei , Norton Peter G. , Leiter Michael P. , Estabrooks Carole A.
Published: July 2024

Background

The Maslach Burnout Inventory-General Survey (MBI-GS) stands as the preeminent tool for assessing burnout across various professions. Although the MBI-GS9 emerged as a derivative of the MBI-GS and has seen extensive use over several years, a comprehensive examination of its psychometric properties has yet to be undertaken.

Methods

This study followed the Standards for Educational and Psychological Testing guidelines to validate the MBI-GS9. Employing a combined approach of classical test theory and item response theory, particularly Rasch analysis, within an integrated framework, the study analyzed data from 16,132 participants gathered between 2005 and 2015 by the Centre for Organizational Research at Acadia University.

Results

The findings revealed that the MBI-GS9 exhibited satisfactory reliability and validity akin to its predecessor, the MBI-GS. Across its three dimensions, Cronbach’s α and omega coefficients ranged from 0.84 to 0.91. Notably, the MBI-GS9 displayed no floor/ceiling effects and demonstrated good item fit, ordered threshold, acceptable person and item separation and reliability, clear item difficulty hierarchy, and a well-distributed item threshold. However, the results suggested a recommended minimum sample size of 350 to mitigate potential information loss when employing the MBI-GS9. Beyond this threshold, the observed mean difference between the MBI-GS and MBI-GS9 held minimal practical significance. Furthermore, measurement equivalence tests indicated that the MBI-GS9 maintained an equivalent three-factor structure and factor loadings across various gender, age, and continent groups, albeit with inequivalent latent values across continents.

Conclusion

In sum, the MBI-GS9 emerges as a reliable and valid alternative to the MBI-GS, particularly when utilized within large, diverse samples across different age and gender demographics. However, to address potential information loss, a substantial sample size is recommended when employing the MBI-GS9. In addition, for cross-cultural comparisons, it is imperative to initially assess equivalence across different language versions at both the item and scale levels.