bluuebunny/crossref_metadata_2025 huggingface dataset:
Socioeconomic status and barriers for contacting the general practitioner when bothered by erectile dysfunction: a population-based cross-sectional study
Authors: Rasmussen Sanne , Balasubramaniam Kirubakaran , Jarbøl Dorte Ejg , Søndergaard Jens , Haastrup Peter Fentz
Published: August 2020

Background

Erectile dysfunction (ED) is common and impacts psychosocial wellbeing negatively. Many do not seek medical attention and several barriers for healthcare seeking with ED exist. Little is known about the association between socioeconomic characteristics of the patient and barriers for healthcare-seeking for men bothered by ED.

The objectives of the study were 1) to estimate the proportion of men bothered by ED, who do not contact the GP, 2) to analyse the frequencies of selected barriers for healthcare seeking and 3) to analyse associations between socioeconomic factors and barriers for contacting the GP.

Methods

Data derive from a nationwide survey of symptom experiences among 100,000 randomly selected individuals aged 20 years and above. The questionnaire comprises, among other, questions about ED. This study focuses on men who reported bothersome ED and further reported, that they did not contact a GP regarding the symptom. Questions addressing barriers regarding GP contact included embarrassment, worrying about wasting the doctor’s time, being too busy, and worrying about what the doctor might find. Information about socioeconomic characteristics was obtained from Statistics Denmark.

Results

A total of 4072 men (18.3%) reported that they had experienced ED within the past four weeks. Of those, 2888 (70.9%) were categorized as having bothersome ED. In the group of men with bothersome ED 1802 (62.4%) did not contact the GP and 60.5% reported barriers for GP-contact. Of the reported barriers, the most frequent was ‘being too embarrassed’ (29.7%). In general, respondents in the older age groups were less likely to report embarrassment, business and worrying what the doctor might find. Respondents with highest attained educational level were less likely to report embarrassment and worrying.

Conclusion

Nearly two third of the respondents with bothersome ED had not contacted their GP. More than half of those reported barriers towards GP contact with embarrassment as the most frequent barrier. In general, respondents in the older age groups and with high educational level were less likely to report barriers.
Correction to: CRISPR-Cas9 mediated targeted disruption of FAD2–2 microsomal omega-6 desaturase in soybean (Glycine max.L)
Authors: al Amin Noor , Ahmad Naveed , Wu Nan , Pu Xiumin , Ma Tong , Du Yeyao , Bo Xiaoxue , Wang Nan , Sharif Rahat , Wang Piwu
Published: August 2020

An amendment to this paper has been published and can be accessed via the original article.
Experiences of participation in bereavement groups from significant others’ perspectives; a qualitative study
Authors: Näppä Ulla , Björkman-Randström Kerstin
Published: August 2020

Background

When death ends a life, the impact of caring for person who suffered a period of illness or disease continues for significant others who are left to grieve. They should be offered support to avoid complicated grief. This can be provided in different ways and individually or in groups. This study aims to describe significant others’ experiences of participation in bereavement groups.

Methods

Ten bereavement groups that each met five times offered support for the significant others of deceased loved ones who had been cared for by a palliative-care team. After the five meetings, the grieving members (n = 46) completed written comments about the role of the groups; they also commented one year after participating (n = 39). Comments were analyzed with qualitative content analysis with a directed approach using the theory of a good death according to the 6S’s: self-image, self-determination, social relationships, symptom control, synthesis and summation, and surrender.

Results

Bereavement groups were found to be a source for alleviating grief for some significant others, but not all experienced relief. Moreover, grief was found to persist during participation. Another finding involved the impact of the role of the palliative home-care team on bereavement support. To evaluate the experience of participating in a bereavement group, the use the 6S’s as a model was a strength of the analysis. Bereavement groups could enhance the self and offer relief from grief. Participation was described as social relationships that offered a sense of coherence and understanding in grief. The effects of participation were more meaningful close to the loss and could lose efficacy over time. Bereavement support provided before a loved one’s death was seen as valuable.

Conclusion

Overall, the bereavement groups eased the grief of significant others close to the death of their loved one. However, moving forward, several of the significant others were not sure that their participation eased their grief. To identify persons who may remain in a state of complicated grief, a routine of planned contacts with the bereaved should begin before death and be followed up later than six months after the death of a loved one.
Breast and prostate cancers harbor common somatic copy number alterations that consistently differ by race and are associated with survival
Authors: Chen Yalei , Sadasivan Sudha M. , She Ruicong , Datta Indrani , Taneja Kanika , Chitale Dhananjay , Gupta Nilesh , Davis Melissa B. , Newman Lisa A. , Rogers Craig G. , Paris Pamela L. , Li Jia , Rybicki Benjamin A. , Levin Albert M.
Published: August 2020

Background

Pan-cancer studies of somatic copy number alterations (SCNAs) have demonstrated common SCNA patterns across cancer types, but despite demonstrable differences in aggressiveness of some cancers by race, pan-cancer SCNA variation by race has not been explored. This study investigated a) racial differences in SCNAs in both breast and prostate cancer, b) the degree to which they are shared across cancers, and c) the impact of these shared, race-differentiated SCNAs on cancer survival.

Methods

Utilizing data from The Cancer Genome Atlas (TCGA), SCNAs were identified using GISTIC 2.0, and in each tumor type, differences in SCNA magnitude between African Americans (AA) and European Americans (EA) were tested using linear regression. Unsupervised hierarchical clustering of the copy number of genes residing in race-differentiated SCNAs shared between tumor types was used to identify SCNA-defined patient groups, and Cox proportional hazards regression was used to test for association between those groups and overall/progression-free survival (PFS).

Results

We identified SCNAs that differed by race in breast (n = 58 SCNAs; permutation p < 10− 4) and prostate tumors (n = 78 SCNAs; permutation p = 0.006). Six race-differentiated SCNAs common to breast and prostate found at chromosomes 5q11.2-q14.1, 5q15-q21.1, 8q21.11-q21.13, 8q21.3-q24.3, 11q22.3, and 13q12.3-q21.3 had consistent differences by race across both tumor types, and all six were of higher magnitude in AAs, with the chromosome 8q regions being the only amplifications. Higher magnitude copy number differences in AAs were also identified at two of these race-differentiated SCNAs in two additional hormonally-driven tumor types: endometrial (8q21.3-q24.3 and 13q12.3-q21.3) and ovarian (13q12.3-q21.3) cancers. Race differentiated SCNA-defined patient groups were significantly associated with survival differences in both cancer types, and these groups also differentiated within triple negative breast cancers based on PFS. While the frequency of the SCNA-defined patient groups differed by race, their effects on survival did not.

Conclusions

This study identified race-differentiated SCNAs shared by two related cancers. The association of SCNA-defined patient groups with survival demonstrates the clinical significance of combinations of these race-differentiated genomic aberrations, and the higher frequency of these alterations in AA relative to EA patients may explain racial disparities in risk of aggressive breast and prostate cancer.
Developing co-funded multi-sectoral partnerships for chronic disease prevention: a qualitative inquiry into federal governmental public health staff experience
Authors: Johnston Lee M. , Goldsmith Laurie J. , Finegood Diane T.
Published: August 2020

Background

Multi-sectoral partnerships (MSPs) are frequently cited as a means by which governments can improve population health while leveraging the resources and expertise of the private and non-profit sectors. As part of their efforts in this area, the Public Health Agency of Canada (the Agency) introduced a novel funding programme requiring applicants to procure matched resources from private sources to support large-scale interventions for chronic disease prevention. The current literature on MSPs is limited in its applicability to this model of multi-sectoral engagement. The purpose of this study was to explore the experiences of Agency staff working with potential partners to develop programme applications, such that we might identify lessons from adopting this type of partnership approach.

Methods

Semi-structured interviews were conducted with the 12 staff working in the MSP programme. Interviews were recorded, transcribed and analysed using thematic analysis. Preliminary themes were used to inform follow up focus-groups sessions. A second round of analysis was conducted guided by a coding paradigm focused on understanding process.

Results

We identified “experiencing uncertainty” to be a central concept in participants’ accounts of the MSP process, related specifically to the MSP programme’s novel conditions, shifts that occurred in sectoral roles and demands for new capacities. In response, Agency staff employed strategies to clarify partner interests, build trust in inter-sectoral relationships, and support internal and partner capacity. Outcomes associated with this process include impacts on trust between the Agency and potential partners, a deeper understanding of other sectors, and programme adaptations and refinements to address challenges related to the programme model.

Conclusions

The co-funding model employed by the Agency is a potentially popular one for government bodies wanting to leverage funding from private sector sources. Our study identifies the potential challenges that can occur under this model. Some challenges are related to addressing material conditions related to partner capacity, whereas other challenges speak to deeper and more difficult to address concerns regarding trust and alignment of motivations and interests between partners. Future research exploring the challenges associated with specific models of MSP engagement is necessary to inform approaches to addressing complex problems through collaborative efforts.
Evaluation of the analgesic effect of vertebral cancellous bone infiltration anaesthesia during vertebroplasty
Authors: Zhang Zhaofei , Jiao Feng , Feng Yonghui , Xie Chunliang , Qin Fengwei , Zhang Sineng , Liu Donghua , Tang Wang
Published: August 2020

Objective

To evaluate the analgesic effect of vertebral cancellous bone infiltration anaesthesia during percutaneous vertebroplasty (PVP).

Methods

Patients treated with vertebral cancellous bone infiltration anaesthesia (intervention group) or local anaesthesia alone (control group) during PVP at our institution during 2016–2018 were reviewed. The visual analogue scale (VAS) score before the operation, during establishment of the puncture channel, during pressure changes in the vertebral body (e.g., when removing or inserting pushers or needle cores), during bone cement injection, immediately after the operation, and at 2 h and 1 day postoperatively were compared between the groups. The patient’s satisfaction with the operation was recorded and compared between groups.

Results

A total of 112 patients were enrolled (59 cases in the intervention group and 53 cases in the control group). There was no difference in the VAS score between the groups before the operation or during establishment of the intraoperative puncture channel (P > 0.05). The VAS score in the intervention group was significantly lower than that in the control group during pressure changes in the vertebral body (removal or insertion of puncture needle cores or pushers) and bone cement injection (P < 0.05). Immediately after the operation and at 2 h postoperatively, the pain in the intervention group was also significantly lower than that in the control group (P < 0.05), but there was no significant difference between the groups at 1 day postoperatively (P > 0.05). The patient satisfaction rate was 88% (52/59) in the intervention group and 67% (35/53) in the control group (P < 0.05).

Conclusions

Vertebral cancellous bone infiltration anaesthesia may effectively relieve intraoperative pain and improve the surgical experience of patients without affecting the clinical effect of surgery.
Intraoperative intra-aortic balloon pump improves 30-day outcomes of patients undergoing extensive coronary endarterectomy
Authors: Wu Zhen , Liu Changcheng , Fang Ying , Wei Hua , Gu Chengxiong
Published: August 2020

Background

The efficacy of intra-aortic balloon pump (IABP) has been proven in high-risk patients undergoing coronary artery bypass grafting (CABG). However, data on the timing and benefits of IABP support in diffuse coronary artery disease after CABG combined with coronary endarterectomy (CE) remain scarce. This retrospective study assessed the effect of intraoperative or postoperative IABP on 30-day outcomes of off-pump CABG+CE.

Methods

From January 2012 to December 2018, 546 patients undergone off-pump CABG+CE were divided into control group (n = 437) and IABP group (n = 109). Risk factors for 30-day outcomes were evaluated. Subgroup analysis from IABP group was conducted to identify the effect of timing IABP on 30-day outcomes.

Results

CE on left anterior descending branch of coronary artery (LAD) (OR = 3.079, 95% CI 1.077–8.805, P = 0.036), CE with≥2 vessels (OR = 9.123, 95% CI 3.179–26.033, P < 0.001) and length of atherosclerotic plaque ≥3 cm (OR = 16.017, 95% CI 5.941–43.183, P < 0.001) were independent risk factors for postoperative acute myocardial infarction (AMI) and 30-day mortality. Comparing with intraoperative IABP support, postoperative IABP support (OR = 3.987, 95% CI1.194–13.317, P = 0.025) was closely associated with postoperative AMI and 30-day mortality.

Conclusions

For patients undergone off-pump CABG and extensive CE (CE on LAD, CE ≥2 vessels and length of atherosclerotic plaque ≥3 cm), intraoperative IABP support may improve 30-day outcomes.
Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome: a comprehensive update
Authors: Herlin Morten Krogh , Petersen Michael Bjørn , Brännström Mats
Published: August 2020

Background

Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome, also referred to as Müllerian aplasia, is a congenital disorder characterized by aplasia of the uterus and upper part of the vagina in females with normal secondary sex characteristics and a normal female karyotype (46,XX).

Main body

The diagnosis is often made during adolescence following investigations for primary amenorrhea and has an estimated prevalence of 1 in 5000 live female births. MRKH syndrome is classified as type I (isolated uterovaginal aplasia) or type II (associated with extragenital manifestations). Extragenital anomalies typically include renal, skeletal, ear, or cardiac malformations. The etiology of MRKH syndrome still remains elusive, however increasing reports of familial clustering point towards genetic causes and the use of various genomic techniques has allowed the identification of promising recurrent genetic abnormalities in some patients. The psychosexual impact of having MRKH syndrome should not be underestimated and the clinical care foremost involves thorough counselling and support in careful dialogue with the patient. Vaginal agenesis therapy is available for mature patients following therapeutical counselling and education with non-invasive vaginal dilations recommended as first-line therapy or by surgery. MRKH syndrome involves absolute uterine factor infertility and until recently, the only option for the patients to achieve biological motherhood was through gestational surrogacy, which is prohibited in most countries. However, the successful clinical trial of uterus transplantation (UTx) by a Swedish team followed by the first live-birth in September, 2014 in Gothenburg, proofed the first available fertility treatment in MRKH syndrome and UTx is now being performed in other countries around the world allowing women with MRKH syndrome to carry their own child and achieve biological motherhood.

Conclusion

Several advances in research across multiple disciplines have been made in the recent years and this kaleidoscopic review provides a current status of various key aspects in MRKH syndrome and provides perspectives for future research and improved clinical care.
Screening for Fabry disease in unknown origin axonal polyneuropathy: to do or not to do, this is the question!
Authors: Rota Eugenia , Grandis Marina , Di Sapio Alessia , Ghiglione Elisabetta , Fiorentino Pietro , Repetto Alessandra , Giliberto Claudia , Gemelli Chiara , Morelli Nicola , Schenone Angelo , Cocito Dario
Published: August 2020

Fabry disease (FD) is a systemic X-linked lysosomal disorder. A ‘peripheral nerve variant’ of FD has been hypothesized in subjects with neuropathy, without the early manifestations of the classic phenotype. A cohort of undiagnosed neuropathy patients with chronic polyneuropathy of undetermined aetiology and demyelinating neuropathy, unresponsive to immunomodulating treatment, were screened for FD. A total of 103 patients (64% males), were enrolled. No typical pathogenetic mutations for FD were identified. We are aware that the study sample was very small, but only a large, unfeasible theoretical sample size could demonstrate a statistically significant increased prevalence of FD in neuropathy patients, as peripheral neuropathy of undetermined cause is uncommon and there is a low prevalence of FD in the general population. Therefore, we are of the opinion that including tailored FD screening in the neuropathy diagnostic work-up, particularly when there are additional clinical characteristics, should be considered.
Characterization of virulence genes in Escherichia coli strains isolated from pre-weaned calves in the Republic of Korea
Authors: Ryu Ji-Hyoung , Kim SuHee , Park Jinho , Choi Kyoung-Seong
Published: August 2020

Background

Escherichia coli is an important cause of diarrhea in calves and its diarrheagenic properties are related to presence of certain virulence genes. In this study, the prevalence of virulence genes F5, F17, F41, sta, stx1, stx2, eae, and saa in E. coli isolated from pre-weaned calves presenting with (n= 329) or without diarrhea (n= 360) was explored using multiplex polymerase chain reaction. We also evaluated the association between detection of E. coli and the presence of diarrhea.

Results

Escherichia coli was detected in 56.3% (388/689) of the fecal samples and showed the highest prevalence (66.5%) in 21–40-day-old calves and the lowest (46.3%) among those that were 1–20 days old. The prevalence of the enterotoxigenic E. coli (ETEC) and Shiga toxin-producing E. coli (STEC) pathotypes was detected in 73.9% and 15.9%, respectively. The results showed no association between diarrhea and the presence of E. coli in general, ETEC or STEC. The F17 gene was the most frequently detected virulence factor in E. coli of calves of all ages regardless of diarrhea. Interestingly, the results show that the calves aged 41–60 days with F17-positive E. coli are at a higher risk for production of Shiga toxin (Stx1; 95% confidence intervals: 1.86–31.95; P = 0.005) compared to calves aged 1–20 days; no association between this finding and diarrhea was observed among the calves of this age group. Moreover, the virulence genes associated with the ETEC and STEC strains were not significantly associated with pathogenicity in this study cohort.

Conclusions

These results suggest that while the incidence of E. coli is age-related, there was no relationship linking E. coli virulence genes to calf age and diarrhea. Furthermore, the present study demonstrated that detection of E. coli strains either with or without virulence factors was not associated with diarrhea in pre-weaned calves.
Applying a general systems theory framework in mental health treatment pathways: the case of the Hellenic Center of Mental Health and Research
Authors: Katrakazas Panagiotis , Grigoriadou Aliki , Koutsouris Dimitrios
Published: August 2020

Language, socio-emotional and cognitive development in children and adolescents with mental health issues is getting increased attention over the last years. Establishing communication patterns and addressing behavioural diversities among this population should be of priority, along with a better understanding in a large variety of patient characteristics within the operational framework of mental healthcare centers. Therefore, the relationships between provided services and operational capability should become more evident. As integrated systems’ approaches are still missing to predict the efficiency of treatment services in a macroscopic scale, a General Systems Theory framework is hereby proposed. This framework is applied and tested against the operational framework of the Hellenic Center of Mental Health and Research, in order to identify the need of such an approach and the strong cooperation between medical and population interactions. Using such frameworks as a prerequisite to identify important factors affecting population states can lead to evaluating their impact on the treatment outcome and depict the complexity of pathways potentially related to the children’s development.
Daratumumab, bortezomib, and dexamethasone in relapsed or refractory multiple myeloma: subgroup analysis of CASTOR based on cytogenetic risk
Authors: Weisel Katja , Spencer Andrew , Lentzsch Suzanne , Avet-Loiseau Hervé , Mark Tomer M. , Spicka Ivan , Masszi Tamas , Lauri Birgitta , Levin Mark-David , Bosi Alberto , Hungria Vania , Cavo Michele , Lee Je-Jung , Nooka Ajay , Quach Hang , Munder Markus , Lee Cindy , Barreto Wolney , Corradini Paolo , Min Chang-Ki , Chanan-Khan Asher A. , Horvath Noemi , Capra Marcelo , Beksac Meral , Ovilla Roberto , Jo Jae-Cheol , Shin Ho-Jin , Sonneveld Pieter , Casneuf Tineke , DeAngelis Nikki , Amin Himal , Ukropec Jon , Kobos Rachel , Mateos Maria-Victoria
Published: August 2020

Background

Multiple myeloma (MM) patients with high cytogenetic risk have poor outcomes. In CASTOR, daratumumab plus bortezomib/dexamethasone (D-Vd) prolonged progression-free survival (PFS) versus bortezomib/dexamethasone (Vd) alone and exhibited tolerability in patients with relapsed or refractory MM (RRMM).

Methods

This subgroup analysis evaluated D-Vd versus Vd in CASTOR based on cytogenetic risk, determined using fluorescence in situ hybridization and/or karyotype testing performed locally. High-risk patients had t(4;14), t(14;16), and/or del17p abnormalities. Minimal residual disease (MRD; 10−5 sensitivity threshold) was assessed via the clonoSEQ® assay V2.0. Of the 498 patients randomized, 40 (16%) in the D-Vd group and 35 (14%) in the Vd group were categorized as high risk.

Results

After a median follow-up of 40.0 months, D-Vd prolonged median PFS versus Vd in patients with standard (16.6 vs 6.6 months; HR, 0.26; 95% CI, 0.19-0.37; P < 0.0001) and high (12.6 vs 6.2 months; HR, 0.41; 95% CI, 0.21–0.83; P = 0.0106) cytogenetic risk. D-Vd achieved deep responses, including higher rates of MRD negativity and sustained MRD negativity versus Vd, regardless of cytogenetic risk. The safety profile was consistent with the overall population of CASTOR.

Conclusion

These updated data reinforce the effectiveness and tolerability of daratumumab-based regimens for RRMM, regardless of cytogenetic risk status.

Trial registration

ClinicalTrials.gov, NCT02136134. Registered 12 May 2014
DNA polymerase stalling at structured DNA constrains the expansion of short tandem repeats
Authors: Murat Pierre , Guilbaud Guillaume , Sale Julian E.
Published: August 2020

Background

Short tandem repeats (STRs) contribute significantly to de novo mutagenesis, driving phenotypic diversity and genetic disease. Although highly diverse, their repetitive sequences induce DNA polymerase slippage and stalling, leading to length and sequence variation. However, current studies of DNA synthesis through STRs are restricted to a handful of selected sequences, limiting our broader understanding of their evolutionary behaviour and hampering the characterisation of the determinants of their abundance and stability in eukaryotic genomes.

Results

We perform a comprehensive analysis of DNA synthesis at all STR permutations and interrogate the impact of STR sequence and secondary structure on their genomic representation and mutability. To do this, we developed a high-throughput primer extension assay that allows monitoring of the kinetics and fidelity of DNA synthesis through 20,000 sequences comprising all STR permutations in different lengths. By combining these measurements with population-scale genomic data, we show that the response of a model replicative DNA polymerase to variously structured DNA is sufficient to predict the complex genomic behaviour of STRs, including abundance and mutational constraints. We demonstrate that DNA polymerase stalling at DNA structures induces error-prone DNA synthesis, which constrains STR expansion.

Conclusions

Our data support a model in which STR length in eukaryotic genomes results from a balance between expansion due to polymerase slippage at repeated DNA sequences and point mutations caused by error-prone DNA synthesis at DNA structures.
Nitric oxide debilitates the neuropathogenic schistosome Trichobilharzia regenti in mice, partly by inhibiting its vital peptidases
Authors: Macháček Tomáš , Šmídová Barbora , Pankrác Jan , Majer Martin , Bulantová Jana , Horák Petr
Published: August 2020

Background

Avian schistosomes, the causative agents of human cercarial dermatitis (or swimmer’s itch), die in mammals but the mechanisms responsible for parasite elimination are unknown. Here we examined the role of reactive nitrogen species, nitric oxide (NO) and peroxynitrite, in the immune response of mice experimentally infected withTrichobilharzia regenti, a model species of avian schistosomes remarkable for its neuropathogenicity.

Methods

Inducible NO synthase (iNOS) was localized by immunohistochemistry in the skin and the spinal cord of mice infected byT. regenti. The impact of iNOS inhibition by aminoguanidine on parasite burden and growth was then evaluatedin vivo. The vulnerability ofT. regentischistosomula to NO and peroxynitrite was assessedin vitroby viability assays and electron microscopy. Additionally, the effect of NO on the activity ofT. regentipeptidases was tested using a fluorogenic substrate.

Results

iNOS was detected around the parasites in the epidermis 8 h post-infection and also in the spinal cord 3 days post-infection (dpi). Inhibition of iNOS resulted in slower parasite growth 3 dpi, but the opposite effect was observed 7 dpi. At the latter time point, moderately increased parasite burden was also noticed in the spinal cord.In vitro, NO did not impair the parasites, but inhibited the activity ofT. regenticathepsins B1.1 and B2, the peptidases essential for parasite migration and digestion. Peroxynitrite severely damaged the surface tegument of the parasites and decreased their viabilityin vitro, but rather did not participate in parasite clearancein vivo.

Conclusions

Reactive nitrogen species, specifically NO, do not directly killT. regentiin mice. NO promotes the parasite growth soon after penetration (3 dpi), but prevents it later (7 dpi) when also suspends the parasite migration in the CNS. NO-related disruption of the parasite proteolytic machinery is partly responsible for this effect.
Animal models of disuse-induced bone loss: study protocol for a systematic review
Authors: Brent Mikkel Bo , Brüel Annemarie , Thomsen Jesper Skovhus
Published: August 2020

Background

Disuse is a cardinal sign of various neurological diseases like stroke, cerebral palsy, and amyotrophic lateral sclerosis. Disuse leads to reduced mechanical loading of the skeleton, and a substantial and significant loss of bone mass quickly materializes. Several animal models have been proposed to investigate the pathogenesis of disuse-induced bone loss and to test new pharmaceutical targets to counteract it. As animal models may overcome several of the limitations in observational studies conducted in patients and allow for measurements not possible in humans, the primary objective of the present study is to provide a comprehensive overview of the available animal models of disuse-induced bone loss.

Methods/design

This is a protocol for a systematic review of animal models of disuse-induced bone loss. An exhaustive search will be performed on PubMed and Embase in order to identify relevant studies. The primary outcome will be the method of disuse induction. The secondary outcomes will be related to bone samples and anatomical sites investigated, methods used to analyze and quantify bone loss, and bibliographic information. The protocol adheres to the current guiding principles of the Preferred Reporting Items for Systematic Review and Meta-analysis Protocols (PRISMA-P) 2015 statement. Extracted data will be analyzed with descriptive statistics, and all the methods used to induce disuse will be described in detail with a narrative synthesis.

Discussion

This systematic review will provide an overview of available animal models of disuse-induced bone loss and discuss the different methods used to quantify and analyze the bone loss. Since bone loss caused by disuse is a hallmark of various diseases from different medical specialties, this overview will be of great benefit for all researchers planning to conduct disuse animal studies in the future.

Systematic review registration

PROSPERO CRD42020157452.
Barriers and facilitators to access mental health services among refugee women in high-income countries: study protocol for a systematic review
Authors: DeSa Sarah , Gebremeskel Akalewold T. , Yaya Sanni
Published: August 2020

Background

According to the United Nation High Commissioner for Refugee Global Trends report in 2019, on average, there are 2.7 refugees per 1000 national population in high-income countries, where girls and women attributed to 48% of the refugee population. Evidence shows high prevalence of mental health disorder among women refugees in comparison to the general population. To our knowledge, no systematic reviews have addressed access to mental health services for refugee women. The aim of this study will be to examine existing barriers and facilitators to accessing mental health services for refugee women in leading high-income countries for refugee resettlement.

Methods

We designed and registered a study protocol for a systematic review. We will conduct a literature search (from inception onwards) in MEDLINE, EMBASE, PsycINFO, and CINAHL. Research articles having a qualitative component (i.e., qualitative, mixed, or multi-method) will be eligible. Study populations of interest will be refugee women at any age that can receive mental health services in leading high-income countries for refugee resettlement (e.g., 14 countries from North America, Europe, and Oceania). Eligibility will be restricted to studies published in English. The primary outcome will be all barriers and facilitators related to accessing mental health services. Two reviewers will independently screen all citations, full-text articles, and abstract data. Potential conflicts will be resolved through discussion. The study methodological quality (or bias) will be appraised using appropriate tools. Reporting will follow the Enhancing Transparency in Reporting the Synthesis of Qualitative Research (ENTREQ) statement. A narrative synthesis will be conducted, and summary of findings tables will be produced. As it will be a systematic review, without human participants’ involvement, there will be no requirement for ethical approval.

Discussion

The systematic review will present key evidence on barriers and facilitators to access mental health services among refugee women in leading resettlement countries. The findings will be used to inform program developers, policymakers, and other stakeholders to enhance mental health services for refugee women. The final manuscript will be disseminated through a peer-reviewed journal and scientific conferences.

Systematic review registration

PROSPERO CRD42020180369.
Correction to: Microbiome definition re-visited: old concepts and new challenges
Authors: Berg Gabriele , Rybakova Daria , Fischer Doreen , Cernava Tomislav , Vergès Marie-Christine Champomier , Charles Trevor , Chen Xiaoyulong , Cocolin Luca , Eversole Kellye , Corral Gema Herrero , Kazou Maria , Kinkel Linda , Lange Lene , Lima Nelson , Loy Alexander , Macklin James A. , Maguin Emmanuelle , Mauchline Tim , McClure Ryan , Mitter Birgit , Ryan Matthew , Sarand Inga , Smidt Hauke , Schelkle Bettina , Roume Hugo , Kiran G. Seghal , Selvin Joseph , de Souza Rafael Soares Correa , van Overbeek Leo , Singh Brajesh K. , Wagner Michael , Walsh Aaron , Sessitsch Angela , Schloter Michael
Published: August 2020

An amendment to this paper has been published and can be accessed via the original article.
Blind versus open weighing from an eating disorder patient perspective
Authors: Froreich Franzisca V. , Ratcliffe Sarah E. , Vartanian Lenny R.
Published: August 2020

Background

Weighing is a key component in the treatment of eating disorders. Most treatment protocols advocate for open weighing, however, many clinicians choose to use blind weighing, especially during the early phase of treatment. Despite considerable debate about this issue in the literature, there is no empirical evidence supporting the superiority of one weighing approach over the other. In addition, little is known about patients’ perspectives of open and blind weighing and which weighing practice they view as more acceptable and/or beneficial for their treatment.

Methods

Semi-structured qualitative interviews were conducted with 41 women with a current or past diagnosis of Anorexia or Bulimia Nervosa: 26 were undergoing specialist inpatient treatment (n = 13 being blind weighed;n = 13 being open weighed) and 15 were community members who have recovered from an eating disorder. Interviews were audiotaped, transcribed verbatim and analysed thematically using framework methods. Participant demographics, clinical characteristics, weighing anxiety and weight concerns were also assessed.

Results

Qualitative analyses yielded five themes:(1) therapy engagement and progress; (2) Control and tolerance of weight uncertainty;(3) treatment team relationships and autonomy; (4) life outside of treatment; and(5) weighing practice preferences and rationale.Participants stated that blind weighing decreased anxiety and eating disorder psychopathology (e.g., weight preoccupation) and increased treatment responsivity. For many, relinquishing control over their weight facilitated body trust and was a necessary step towards recovery. Participants found that not knowing their exact weight helped challenge their overconcern with weight. Lack of support post-discharge was identified as a major difficulty of blind weighing. Overall, the majority of participants preferred blind weighing, particularly at the early, acute stage of treatment, whereas open weighing was viewed as more suitable at later stages of recovery. Quantitative analyses found current blind-weighed patients felt significantly less anxiety around being weighed and had greater tolerance of weight uncertainty than current open-weighed patients.

Conclusions

This study provided in-depth patient insights into open versus blind weighing practices. The next step for future research will be to supplement these insights with treatment outcomes gained from randomised controlled trials comparing the two weighing practices.
Risk factors for complete recovery of adults after weaning from veno-venous extracorporeal membrane oxygenation for severe acute respiratory failure: an analysis from adult patients in the Extracorporeal Life Support Organization registry
Authors: Yeo Hye Ju , Kim Yun Seong , Kim Dohyung ,   ELSO Registry Committee, Cho Woo Hyun
Published: August 2020

Background

As extracorporeal membrane oxygenation (ECMO) has been widely used, the patient quality of life following ECMO termination has become an important issue as same as the patient’s survival. To date, the factors affecting complete recovery of adult survivors from ECMO have not been investigated.

Methods

Data from adult patients in the Extracorporeal Life Support Organization registry who received veno-venous ECMO between 2012 and 2017 were analyzed. Multivariate logistic regression analyses were conducted.

Results

In total, 6536 patients with 242,183 days of veno-venous ECMO were reviewed. The overall survival to discharge rate after weaning from ECMO was 89.7% (n= 5861), and 10.3% (n= 675) of the patients died during hospitalization. The discharge location varied as follows: 33.7% (n= 1976) returned home, 23.4% (n= 1369) were transferred to a referral hospital, 41.8% (n= 2447) required hospital services, and 0.6% (n= 36) were discharged to other places. The patients were divided into two groups according to the discharge location: a complete recovery group (n= 1976) and a partial recovery group (n= 3885). In the multivariate analyses, age (≥ 65 years) (odds ratio (OR) 0.72, 95% confidence interval (CI) 0.59–0.87,p= 0.001), cardiac arrest before ECMO (OR 0.76, 95% CI 0.60–0.96,p= 0.021), vasopressor use (OR 0.73, 95% CI 0.64–0.83,p< 0.001), renal replacement therapy (OR 0.40, 95% CI 0.28–0.57,p< 0.001), ECMO-related complications (OR 0.76, 95% CI 0.67–0.85,p< 0.001), and long-term ECMO support (≥ 2 weeks) (OR 0.42, 95% CI 0.37–0.48,p< 0.001) were significantly associated with complete recovery.

Conclusion

Complete recovery after veno-venous ECMO support is associated with the patient’s baseline condition, ECMO duration, and ECMO-related complications. Respiratory ECMO should aim to increase both the survival and the quality of life after weaning from ECMO.
Influence of culture conditions on the production of extracellular polymeric substances (EPS) by Arthrospira platensis
Authors: Silva Mariana B. F. , Azero Edwin G. , Teixeira Cláudia M. L. L. , Andrade Cristina T.
Published: August 2020

Arthrospira platensis is a cyanobacterium that is of great biotechnological interest, particularly for the food industry, as it possesses a high content of proteins, pigments, lipids and carbohydrates. Cyanobacteria produce extracellular polymeric substances (EPS), which are co-products of secondary metabolism that present thickening or gelling properties. A 3-level factorial design was used to study the combined effect of different nitrate concentrations and photon flux density (PFD) values to evaluate the biomass and EPS production of A. platensis. The best result in terms of biomass production was obtained under condition 6 (2 g L−1 NaNO3 and 600 μE m−2 s−1) yielding a concentration of 1.292 g L−1. However, condition 1 (0.25 g L−1 NaNO3 and 200 μE m−2 s−1) produced the greatest EPS yield (111 mg g−1), followed by condition 9 (2 g L−1 NaNO3 and 1000 μE m−2 s−1). FTIR analyses of EPS samples indicated the presence of carboxylate and sulfate functional groups, and rheological studies of the EPS at 5 and 10 g L−1 revealed a dilute solution behavior.
Variations in the natural 13C and 15N abundance of plants and soils under long-term N addition and precipitation reduction: interpretation of C and N dynamics
Authors: Yan Guoyong , Han Shijie , Zhou Mingxin , Sun Wenjing , Huang Binbin , Wang Honglin , Xing Yajuan , Wang Qinggui
Published: August 2020

Background

The nitrogen isotope natural abundance (δ15N) provides integrated information on ecosystem N dynamics, and carbon isotope natural abundance (δ13C) has been used to infer how water-using processes of plants change in terrestrial ecosystems. However, how δ13C and δ15N abundances in plant life and soils respond to N addition and water availability change is still unclear. Thus, δ13C and δ15N abundances in plant life and soils were used to investigate the effects of long-time (10 years) N addition (+ 50 kg N·ha− 1·yr− 1) and precipitation reduction (− 30% of throughfall) in forest C and N cycling traits in a temperate forest in northern China.

Results

We analyzed the δ13C and δ15N values of dominant plant foliage, litterfall, fungal sporophores, roots, and soils in the study. The results showed that δ15N values of foliage, litterfall, and surface soil layer’s (0–10 cm) total N were significantly increased by N addition, while δ15N values of fine roots and coarse roots were considerably decreased. Nitrogen addition also significantly increased the δ13C value of fine roots and total N concentration of the surface soil layer compared with the control. The C concentration, δ13C, and δ15N values of foliage and δ15N values of fine roots were significantly increased by precipitation reduction, while N concentration of foliage and litterfall significantly decreased. The combined effects of N addition and precipitation reduction significantly increased the δ13C and δ15N values of foliage as well as the δ15N values of fine roots and δ13C values of litterfall. Furthermore, foliar δ15N values were significantly correlated with foliage δ13C values, surface soil δ15N values, surface soil C concentration, and N concentrations. Nitrogen concentrations and δ13C values of foliage were significantly correlated with δ15N values and N concentrations of fine roots.

Conclusions

This indicates that plants increasingly take up the heavier 15N under N addition and the heavier 13C and 15N under precipitation reduction, suggesting that N addition and precipitation reduction may lead to more open forest ecosystem C and N cycling and affect plant nutrient acquisition strategies.
Regeneration patterns of key pine species in a mixed-pine forest indicate a positive effect of variable retention harvesting and an increase in recruitment with time
Authors: Nyamai Priscilla A. , Goebel P. Charles , Gregory Corace R. , Hix David M.
Published: August 2020

Background

Many fire-dependent forests have experienced significant declines in species, structural, and functional diversity. These changes are attributed in part to traditional management approaches that were dominated by even-aged regeneration methods such as clearcutting. Variable retention harvesting (VRH) is an ecologically based forestry practice that involves retention of some mature overstory trees and other biological structures in the postharvest stand to emulate the effects of natural disturbance events. In this study, we examined the effect of a VRH treatment on recruitment of historically dominant pine species and understory vegetation two- and six years after its implementation in a naturally regenerated mixed pine forest in the Upper Peninsula of Michigan, USA.

Results

We found greater regeneration of red pine (Pinus resinosaL.) and eastern white pine (Pinus strobusL.) in VRH stands compared to the unharvested controls. Although red pine recruitment was very low during the first 2 years, both species increased significantly from year two to year six. Recruitment responses for the two species did not differ significantly between the aggregated and dispersed retention treatments. We also found high recruitment of deciduous tree species, consisting primarily of sprouts, and red pine exhibited a negative correlation with these sprouts. Treatment, time, and the interaction of treatment and time were significant factors in red pine recruitment. Time had a strong effect on the understory vegetation, with significant decreases in shrubs and ferns over time.

Conclusions

The results suggest that VRH enhanced recruitment of red pine and eastern white pine and that this recruitment increased with time. We identify competition from sprouts and unfavorable seedbed conditions as the main factors that limited recruitment of red pine during the first 2 years. To reduce competing vegetation and improve seedbed conditions, we suggest following the VRH with a prescribed fire, mechanically removing sprouts, and implementing herbicide treatments. At a time when changes in global climate exacerbates the effects of traditional drivers of forest degradation, and with the need to maintain biodiversity, new ecologically based forest practices such VRH have the potential to facilitate regeneration of native trees and enhance the resilience of many fire-dependent forests.
Hybrid identity and practices to negotiate belonging: Madrid’s Muslim youth of migrant origin
Authors: Boland Colleen
Published: August 2020

A qualitative case study of self-identifying Muslim youth of migrant origin in Madrid sought to understand how they formulated identity and employed real cultural practices in navigating belonging. The majority of these youth drew from the so-called “1.5” and “second” generations, and expressed a hyphenated or hybrid identity: they combined one or several aspects of religious, cultural or ethnic identity alongside an identification with Spain or Madrid. As such, these layered and complex identities could indicate attachment to community of residence. Hybridity was also manifested in behaviours, with participants demonstrating multiple language use, as well as variegated cultural or religious practice. Moreover, in negotiating inclusion and belonging, some argued for their rights to express these plural identities and engage in diverse cultural practices, particularly when such rights were perceived as denied. This paper primarily contends that participants leveraged hybrid identities in representing themselves and finding common ground: they maintained attachments to Madrid or Spain alongside their minority identification and diverse cultural practices, or incorporated these practices in engaging with their communities. It furthermore observes that despite perceived experiences of discrimination, this population’s claims to membership and respect for individual rights reflected an understanding of citizenship based in participation and recognition of difference, raising questions as to the impact of past or future citizenship policy and education.
Mid-spatial frequency error generation mechanisms and prevention strategies for the grinding process
Authors: Pohl Mario , Kukso Olga , Boerret Rainer , Rascher Rolf
Published: August 2020

The research presented in this paper is focused on the link between manufacturing parameters and the resulting mid-spatial frequency error in the manufacturing process of precision optics. The goal is to understand the generation mechanisms of mid-spatial frequency errors and avoid their appearance in the manufacturing process. Also, a simulation which is able to predict the resulting mid-spatial frequency error from a manufacturing process is developed and verified.
Coil embolization of a thoracic aorta hematoma with branch artery pseudoaneurysm – case report
Authors: Szabo Lorant , Reyes del Castillo Tomas , Benz David , Roos J. , Seelos Robert , Lopez-Benitez Ruben
Published: August 2020

Background

A thoracic aorta hematoma with branch artery pseudonaneurysm is a very rare complication of thoraric blunt trauma. The standard treatment of this type of injury is aortic endograft placement.

Case presentation

We present a case in which a thoracic aorta hematoma with branch artery pseudoaneurysm was treated with coil embolization instead of endografting.

Conclusions

Coil embolization of aortic injuries may be a safe and definitive treatment alternative in selected cases. This technique has the potential to reduce the risk of procedure-related complications.