bluuebunny/crossref_metadata_2025 huggingface dataset:
Regulation Mechanism of ssDNA Aptamer in Nanozymes and Application of Nanozyme-Based Aptasensors in Food Safety
Authors: Wang Lijun , Zhou Hong , Hu Haixia , Wang Qin , Chen Xianggui
Published: February 2022

Food safety issues are a worldwide concern. Pathogens, toxins, pesticides, veterinary drugs, heavy metals, and illegal additives are frequently reported to contaminate food and pose a serious threat to human health. Conventional detection methods have difficulties fulfilling the requirements for food development in a modern society. Therefore, novel rapid detection methods are urgently needed for on-site and rapid screening of massive food samples. Due to the extraordinary properties of nanozymes and aptamers, biosensors composed of both of them provide considerable advantages in analytical performances, including sensitivity, specificity, repeatability, and accuracy. They are considered a promising complementary detection method on top of conventional ones for the rapid and accurate detection of food contaminants. In recent years, we have witnessed a flourishing of analytical strategies based on aptamers and nanozymes for the detection of food contaminants, especially novel detection models based on the regulation by single-stranded DNA (ssDNA) of nanozyme activity. However, the applications of nanozyme-based aptasensors in food safety are seldom reviewed. Thus, this paper aims to provide a comprehensive review on nanozyme-based aptasensors in food safety, which are arranged according to the different interaction modes of ssDNA and nanozymes: aptasensors based on nanozyme activity either inhibited or enhanced by ssDNA, nanozymes as signal tags, and other methods. Before introducing the nanozyme-based aptasensors, the regulation by ssDNA of nanozyme activity via diverse factors is discussed systematically for precisely tailoring nanozyme activity in biosensors. Furthermore, current challenges are emphasized, and future perspectives are discussed.
On a Nonlocal Problem for Mixed-Type Equation with Partial Riemann-Liouville Fractional Derivative
Authors: Ruziev Menglibay , Zunnunov Rakhimjon
Published: February 2022

The present paper presents a study on a problem with a fractional integro-differentiation operator in the boundary condition for an equation with a partial Riemann-Liouville fractional derivative. The unique solvability of the problem is proved. In the hyperbolic part of the considered domain, the functional equation is solved by the iteration method. The problem is reduced to solving the Volterra integro-differential equation.
Regularity of Fractional Heat Semigroup Associated with Schrödinger Operators
Authors: Li Pengtao , Qian Tao , Wang Zhiyong , Zhang Chao
Published: February 2022

Let L=−Δ+V be a Schrödinger operator, where the potential V belongs to the reverse Hölder class. By the subordinative formula, we introduce the fractional heat semigroup {e−tLα}t>0, 0<α<1, associated with L. By the aid of the fundamental solution of the heat equation: ∂tu+Lu=∂tu−Δu+Vu=0, we estimate the gradient and the time-fractional derivatives of the fractional heat kernel Kα,tL(·,·), respectively. This method is independent of the Fourier transform, and can be applied to the second-order differential operators whose heat kernels satisfy the Gaussian upper bounds. As an application, we establish a Carleson measure characterization of the Campanato-type space BMOLγ(Rn) via the fractional heat semigroup {e−tLα}t>0.
Antibacterial Hydrogels Derived from Poly(γ-glutamic acid) Nanofibers
Authors: Kasbiyan Hamidreza , Yousefzade Omid , Simiand Estelle , Saperas Núria , del Valle Luis J. , Puiggalí Jordi
Published: February 2022

Biocompatible hydrogels with antibacterial properties derived from γ-polyglutamic acid (γ-PGA) were prepared from bulk and electrospun nanofibers. The antibacterial drugs loaded in these hydrogels were triclosan (TCS), chlorhexidine (CHX) and polyhexamethylene biguanide (PHMB); furthermore, bacteriophages were loaded as an alternative antibacterial agent. Continuous and regular γ-PGA nanofibers were successfully obtained by the electrospinning of trifluoroacetic acid solutions in a narrow polymer concentration range and restricted parameter values of flow rate, voltage and needle-collector distance. Hydrogels were successfully obtained by using cystamine as a crosslinking agent following previous published procedures. A closed pore structure was characteristic of bulk hydrogels, whereas an open but structurally consistent structure was found in the electrospun hydrogels. In this case, the morphology of the electrospun nanofibers was drastically modified after the crosslinking reaction, increasing their diameter and surface roughness according to the amount of the added crosslinker. The release of TCS, CHX, PHMB and bacteriophages was evaluated for the different samples, being results dependent on the hydrophobicity of the selected medium and the percentage of the added cystamine. A high efficiency of hydrogels to load bacteriophages and preserve their bactericide activity was demonstrated too.
Integrated Hair Follicle Profiles of microRNAs and mRNAs to Reveal the Pattern Formation of Hu Sheep Lambskin
Authors: Lv Xiaoyang , Chen Weihao , Wang Shanhe , Cao Xiukai , Yuan Zehu , Getachew Tesfaye , Mwacharo Joram M. , Haile Aynalem , Sun Wei
Published: February 2022

Hair follicle development is closely associated with wool curvature. Current studies reveal the crucial role of microRNAs (miRNAs) in hair follicle growth and development. However, few studies are known regarding their role in wool curvature. To reveal the potential roles of miRNAs in Hu sheep lambskin with different patterns, a total of 37 differentially expressed (DE) miRNAs were identified in hair follicles between small waves (SM) and straight wool (ST) groups using RNA-seq. Through functional enrichment and miRNA-mRNA co-expression analysis, some key miRNAs (oar-miR-143, oar-miR-200b, oar-miR-10a, oar-miR-181a, oar-miR-10b, oar-miR-125b, etc.) and miRNA-mRNA pairs (miR-125b target CD34, miR-181a target FGF12, LMO3, miR-200b target ZNF536, etc.) were identified. Though direct or indirect ways affecting hair follicle development, these miRNAs and mRNAs may have possible effects on wool curvature, and this study thus provides valuable insight on potential pattern formation.
Developing Non-Human Primate Models of Inherited Retinal Diseases
Authors: Seah Ivan , Goh Debbie , Chan Hwei Wuen , Su Xinyi
Published: February 2022

Inherited retinal diseases (IRDs) represent a genetically and clinically heterogenous group of diseases that can eventually lead to blindness. Advances in sequencing technologies have resulted in better molecular characterization and genotype–phenotype correlation of IRDs. This has fueled research into therapeutic development over the recent years. Animal models are required for pre-clinical efficacy assessment. Non-human primates (NHP) are ideal due to the anatomical and genetic similarities shared with humans. However, developing NHP disease to recapitulate the disease phenotype for specific IRDs may be challenging from both technical and cost perspectives. This review discusses the currently available NHP IRD models and the methods used for development, with a particular focus on gene-editing technologies.
Effect of Intensive Rehabilitation Program in Thermal Water on a Group of People with Parkinson’s Disease: A Retrospective Longitudinal Study
Authors: Di Marco Roberto , Pistonesi Francesca , Cianci Valeria , Biundo Roberta , Weis Luca , Tognolo Lucrezia , Baba Alfonc , Rubega Maria , Gentile Giovanni , Tedesco Chiara , Carecchio Miryam , Antonini Angelo , Masiero Stefano
Published: February 2022

The main objective of this study is to test the effect of thermal aquatic exercise on motor symptoms and quality of life in people with Parkinson’s Disease (PD). Fourteen participants with diagnosis of idiopathic PD completed the whole rehabilitation session and evaluation protocol (Hoehn and Yahr in OFF state: 2–3; Mini Mental State Examination >24; stable pharmacological treatment in the 3 months prior participating in the study). Cognitive and motor status, functional abilities and quality of life were assessed at baseline and after an intensive rehabilitation program in thermal water (12 sessions of 45 min in a 1.4 m depth pool at 32–36 ∘C). The Mini Balance Evaluation System Test (Mini-BESTest) and the PD Quality of Life Questionnaire (PDQ-39) were considered as main outcomes. Secondary assessment measures evaluated motor symptoms and quality of life and psychological well-being. Participants kept good cognitive and functional status after treatment. Balance of all the participants significantly improved (Mini-BESTest: p<0.01). The PDQ-39 significantly improved after rehabilitation (p=0.038), with significance being driven by dimensions strongly related to motor status. Thermal aquatic exercise may represent a promising rehabilitation tool to prevent the impact of motor symptoms on daily-life activities of people with PD. PDQ-39 improvement foreshows good effects of the intervention on quality of life and psychological well-being.
The Mediating Effect of Healthcare Utilization on Livelihood Empowerment against Poverty (LEAP) and Health Security of Older Adults in Ghana: A Case Study on the Ga-West Municipality in Accra, Ghana
Authors: Amarteifio Edwina Naa Amerley , Dai Baozhen , Nutakor Jonathan Aseye , Larnyo Ebenezer
Published: February 2022

The concept of health security has aroused several interpretations because of theoretical technique indifferences or academic methodology. The focus has been on human security, old age health security (OAHS), whereas there remain issues of low healthcare utilization by older people from rural Ghana while there are social assistance programs. The study aimed at investigating the mediating effect of healthcare utilization on Livelihood Empowerment against Poverty (LEAP) and old age health security OAHS. With purposive sampling technique, participants were selected to participate in the study with standardized quantitative questionnaire to measure the variables involved in the study and a regression technique to analyze the data. The result of the mediation analysis showed a partial mediation between LEAP and Healthcare Utilization (HU) was found to have bridged the gap between the LEAP and OAHS. The LEAP policy also caused an increase in Health Utilization and, subsequently, an increase in old age health security (OAHS). The study is essential to help the National Health Insurance Authority NHIA in restructuring health care premiums to incite utilization of health facilities by the aged.
Building a Nomogram for Metabolic Syndrome Using Logistic Regression with a Complex Sample—A Study with 39,991,680 Cases
Authors: Shin Min-Seok , Lee Jea-Young
Published: February 2022

Metabolic syndrome can cause complications, such as stroke and cardiovascular disease. We aimed to propose a nomogram that visualizes and predicts the probability of metabolic syndrome occurrence after identifying risk factors related to metabolic syndrome for prevention and recognition. We created a nomogram related to metabolic syndrome in this paper for the first time. We analyzed data from the Korea National Health and Nutrition Examination Survey VII. Total 17,584 participants were included in this study, and the weighted sample population was 39,991,680, which was 98.1% of the actual Korean population in 2018. We identified 14 risk factors affecting metabolic syndrome using the Rao-Scott chi-squared test. Next, logistic regression analysis was performed to build a model for metabolic syndrome and 11 risk factors were finally obtained, including BMI, marriage, employment, education, age, stroke, sex, income, smoking, family history and age* sex. A nomogram was constructed to predict the occurrence of metabolic syndrome using these risk factors. Finally, the nomogram was verified using a receiver operating characteristic curve (ROC) and a calibration plot.
How Did the Pandemic Affect Communication in Clinical Settings? A Qualitative Study with Critical and Emergency Care Nurses
Authors: Díaz-Agea José Luis , Orcajada-Muñoz Irene , Leal-Costa César , Adánez-Martínez Maria Gracia , De Souza Oliveira Adriana Catarina , Rojo-Rojo Andrés
Published: February 2022

The objective of the present study was to determine the subjective impact of the pandemic due to COVID-19 on communication, as perceived by nurses working at emergency services and Intensive Care Units at various hospitals in the Region of Murcia (Spain). A qualitative study was conducted based on the content analysis of 12 semi-structured individual interviews. The participant recruitment process was performed through a snowball sampling technique. Four main dimensions, eleven categories, and two sub-categories were obtained: (1) communication (communicative expressions, both verbal and non-verbal-, and limitations); (2) emotional aspects (positive, negative); (3) overload (first wave, second wave, and third wave); and (4) relationships (health professionals–patients, healthcare professionals, patients–family, and family–health professionals). The main findings of the study show that communication was slightly affected during the pandemic, especially the non-verbal kind, with verbal communication maintained and, in some occasions, strengthened. The lack of training in communication skills and its influence on the management of difficult periods was another important finding. Communication in general deteriorated during the pandemic, especially during the initial waves. Non-verbal communication was more affected due to the use of Personal Protective Equipment and the initial fear of infection, with this finding strongly observed in departments such as emergencies or critical care. The nurses who were interviewed underlined negative emotional aspects associated with a deficit in communication. The positive aspects described were associated with the creation of mutual support spaces and the group cohesion of the work teams during the pandemic. As an implication for current and future clinical practice, we recommend a coordinated institutional response to mitigate the potential emotional effects on workers by designing appropriate communication and emotional expression protocols.
Effects of Different Scan Projections on the Quantitative Ultrasound-Based Evaluation of Hepatic Steatosis
Authors: De Rosa Laura , Salvati Antonio , Bonino Ferruccio , Brunetto Maurizia Rossana , Faita Francesco
Published: February 2022

Non-alcoholic fatty liver disease (NAFLD) is becoming a global public health issue and the identification of the steatosis severity is very important for the patients’ health. Ultrasound (US) images of 214 patients were acquired in two different scan views (subcostal and intercostal). A classification of the level of steatosis was made by a qualitative evaluation of the liver ultrasound images. Furthermore, an US image processing algorithm provided quantitative parameters (hepatic–renal ratio (HR) and Steato-score) designed to quantifying the fatty liver content. The aim of the study is to evaluate the differences in the assessment of hepatic steatosis acquiring and processing different US scan views. No significant differences were obtained calculating the HR and the Steato-score parameters, not even with the classification of patients on the basis of body mass index (BMI) and of different classes of steatosis severity. Significant differences between the two parameters were found only for patients with absence or mild level of steatosis. These results show that the two different scan projections do not greatly affect HR and the Steato-score assessment. Accordingly, the US-based steatosis assessment is independent from the view of the acquisitions, thus making the subcostal and intercostal scans interchangeable, especially for patients with moderate and severe steatosis.
A Review on Atrial Fibrillation (Computer Simulation and Clinical Perspectives)
Authors: Zaman Muhammad Adib Uz , Du Dongping
Published: February 2022

Atrial fibrillation (AF), a heart condition, has been a well-researched topic for the past few decades. This multidisciplinary field of study deals with signal processing, finite element analysis, mathematical modeling, optimization, and clinical procedure. This article is focused on a comprehensive review of journal articles published in the field of AF. Topics from the age-old fundamental concepts to specialized modern techniques involved in today’s AF research are discussed. It was found that a lot of research articles have already been published in modeling and simulation of AF. In comparison to that, the diagnosis and post-operative procedures for AF patients have not yet been totally understood or explored by the researchers. The simulation and modeling of AF have been investigated by many researchers in this field. Cellular model, tissue model, and geometric model among others have been used to simulate AF. Due to a very complex nature, the causes of AF have not been fully perceived to date, but the simulated results are validated with real-life patient data. Many algorithms have been proposed to detect the source of AF in human atria. There are many ablation strategies for AF patients, but the search for more efficient ablation strategies is still going on. AF management for patients with different stages of AF has been discussed in the literature as well but is somehow limited mostly to the patients with persistent AF. The authors hope that this study helps to find existing research gaps in the analysis and the diagnosis of AF.
Construction of an Intragenic SSR-Based Linkage Map and QTL Mapping for Agronomic Traits in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)
Authors: Gao Hanzhong , Yang Xiaogang , Wang Hongxia , Qiu Nianwei , Chen Yanan , Wang Fengde , Zhang Yihui , Li Huayin , Li Jingjuan , Gao Jianwei
Published: February 2022

Chinese cabbage (Brassica rapa L. ssp. pekinensis) is one of the most widely cultivated and economically important vegetables in China. Constructing an effective genetic linkage map and mapping quantitative trait loci (QTLs) related to yield and leafy head morphology is of great importance for molecular breeding of Chinese cabbage. Using two diverse Chinese cabbage inbred lines, ZHB and G291, as parents, an F2 segregating population consisting of 240 individuals was prepared for genetic map construction and phenotype investigation in this study. The two parents are significantly different in both shape and size. Sixteen important agronomic traits of F2 individuals were investigated. A genetic map of 105 intragenic simple sequence repeat (SSR) markers distributed across 10 linkage groups (LGs) was constructed, which was 2034.1 cM in length and had an average inter-locus distance of 21.75 cM. We identified 48 QTLs for the tested important agronomic traits on the studied LGs, with LOD scores of 2.51–12.49, which explained the phenotypic variance of 3.41–26.66%. The QTLs identified in this study will facilitate further genetic analysis and marker-assisted genetic improvement of Chinese cabbage.
Educators’ Psychosocial Burdens Due to the COVID-19 Pandemic and Predictive Factors: A Cross-Sectional Survey of the Relationship with Sense of Coherence and Social Capital
Authors: Fukuda Yasue , Fukuda Koji
Published: February 2022

This study aims to identify the social and psychological burdens placed on educators during the third wave of the COVID-19 pandemic in Japan and to propose an optimal form of support. We investigated educators’ perceptions of psychological and socioeconomic anxieties and burdens, sense of coherence, and social capital using a questionnaire survey of 1000 educators in January 2021. Multivariate regression analyses were conducted to analyze the associations between the variables. Results: Approximately 80% of the respondents considered COVID-19 a formidable, life-threatening illness. Our results revealed that the higher the social capital, the greater the fear of COVID-19, and the higher the sense of coherence, the lower this fear. Conclusions: The anxiety burden of implementing infection prevention was higher than the anxiety burden associated with distance learning. The predictive factors for educators’ perceptions of burden included sense of coherence, gender, and age. Our findings suggest the importance of having the government and educational institutions provide multidimensional assistance that matches educators’ individual characteristics.
Trends in Lakeshore Zone Development: A Comparison of Polish and Hungarian Lakes over 30-Year Period
Authors: Furgała-Selezniow Grażyna , Jankun-Woźnicka Małgorzata , Woźnicki Paweł , Cai Xuecheng , Erdei Timea , Boromisza Zsombor
Published: February 2022

(1) Background: This paper presents the land use and land cover change processes in the lakeshore zone in Poland and Hungary during 30 years. (2) Methods: Land use and land cover (LU/LC) maps were prepared using topographic maps and orthophotograph maps scaled 1:10,000. The study based on GIS data and field research. (3) Results: A significant increase in the area occupied by tourist and recreational infrastructure and forests in the lake shore zone was found in both countries. In Poland, this increase occurred mainly at the expense of arable land, which was a positive phenomenon. In Hungary, however, the main threat to the lakeshore zone was the increase of built-up area at the expense of semi-natural area. While the decrease in arable land was positive from an environmental point of view, the main threat to the Hungarian lake shore zone was the increase in built-up areas at the expense of semi-natural land. The results showed a positive correlation between the area of urbanized land and the area occupied by tourist and recreational buildings on the Polish lakes. There was no such correlation at the Hungarian lakes. (4) Conclusions: The most beneficial change in land cover for the lakes was the increase in forest area in the lake shore zone in both countries studied. Taking into account the results of previous studies, three main trends of changes in land cover and land use in the lakeshore zone were identified. These results shed new light on the problem of land use around lakeshores.
The Protective Role of Job Control/Autonomy on Mental Strain of Managers: A Cross-Sectional Study among Wittyfit’s Users
Authors: Colin-Chevalier Rémi , Pereira Bruno , Benson Amanda Clare , Dewavrin Samuel , Cornet Thomas , Dutheil Frédéric
Published: February 2022

Background: Karasek’s Job Demand-Control-Support model is the gold standard to assess the perception of work; however, this model has been poorly studied among managers. We aimed to explore the perception of work (job demand, control, and support) in managers, and to quantify their risk of job strain (high job demand and low job control) and isostrain (job strain with low job support). Methods: We conducted a cross-sectional study on workers from various French companies using the Wittyfit software. Job demand, control, and support were evaluated by self-reported questionnaires, as well as sociodemographic data. Results: We included 9257 workers: 8488 employees (median age of 45 years, median seniority of 10 years, 39.4% women) and 769 managers (463 were more than 45 years old, 343 with more than 10 years of service, 33.3% women). Managers had higher mean ± SD levels than employees in job control (79.2 ± 14.9 vs. 75.4 ± 16.9) and job support (25.2 ± 5.1 vs. 24.0 ± 6.1) (p < 0.001). Compared to employees, managers had a 37% decreased risk of job strain (OR = 0.63, 95% CI 0.52 to 0.77) and a 47% decreased risk of isostrain (OR = 0.53, 95% CI 0.40 to 0.69) (p < 0.001). Workers over age 45 (OR = 1.26, 95% CI 1.14 to 1.40, p < 0.001) and women (OR = 1.12, 95% CI 1.01 to 1. 25, p = 0.03) were at greater risk of job strain. Furthermore, workers over age 45 (OR = 1.51, 95% CI 1.32 to 1.73, p < 0.001), workers with over 10 years of service (OR = 1.35, 95% CI 1.16 to 1.56, p < 0.001), and women (OR = 1.15, 95% CI 1.00 to 1.31, p = 0.04) were at greater risk of isostrain. Conclusions: Managers seem to have higher autonomy and greater social support and therefore are less at risk of job strain or isostrain than employees. Other factors such as age, seniority, and sex may influence this relationship. Trial Registration: Clinicaltrials.gov: NCT02596737.
EEG-Based Identification of Emotional Neural State Evoked by Virtual Environment Interaction
Authors: Jung Dawoon , Choi Junggu , Kim Jeongjae , Cho Seoyoung , Han Sanghoon
Published: February 2022

Classifying emotional states is critical for brain–computer interfaces and psychology-related domains. In previous studies, researchers have tried to identify emotions using neural data such as electroencephalography (EEG) signals or brain functional magnetic resonance imaging (fMRI). In this study, we propose a machine learning framework for emotion state classification using EEG signals in virtual reality (VR) environments. To arouse emotional neural states in brain signals, we provided three VR stimuli scenarios to 15 participants. Fifty-four features were extracted from the collected EEG signals under each scenario. To find the optimal classification in our research design, three machine learning algorithms (XGBoost classifier, support vector classifier, and logistic regression) were applied. Additionally, various class conditions were used in machine learning classifiers to validate the performance of our framework. To evaluate the classification performance, we utilized five evaluation metrics (precision, recall, f1-score, accuracy, and AUROC). Among the three classifiers, the XGBoost classifiers showed the best performance under all experimental conditions. Furthermore, the usability of features, including differential asymmetry and frequency band pass categories, were checked from the feature importance of XGBoost classifiers. We expect that our framework can be applied widely not only to psychological research but also to mental health-related issues.
Anxiety, Stress, and Resilience Strategies in Parents of Children with Typical and Late Psychosocial Development: Comparative Analysis
Authors: Andrioni Felicia , Coman Claudiu , Ghita Roxana-Catalina , Bularca Maria Cristina , Motoi Gabriela , Fulger Ioan-Valentin
Published: February 2022

The child’s developmental characteristics influence the psycho-social features in the behavior of parents. This aspect is relevant in building effective strategies for psychological and socio-educational assistance to parents for an increased quality of family life. The aim of the present study is to investigate the differences in anxiety, stress, and resilience strategies in the case of parents with children with late psychosocial development and those with children with neurotypical development. The research sample consisted of 620 subjects (380 women, 240 men, divided into two equal categories: parents of children with late development and with neurotypical development). The questionnaire survey (Levenstein’s Perceived Stress Questionnaire for stress and Hobfoll’s Strategic Approach to Coping Scale for resilience strategies) and interview (Hamilton’s Anxiety Rating Scale for anxiety) were used. The anxiety levels did not differ significantly (t = 0.45, p = 0.65), but there were differences in the perceived stress levels (t = −7.10, p = 0.03). As for resilience strategies, significant differences were found for assertive action, social communion, precautionary action, and seeking social support, which were more pronounced strategies that were used by parents of children with late psychosocial development.
Identifying Urban Wetlands through Remote Sensing Scene Classification Using Deep Learning: A Case Study of Shenzhen, China
Authors: Yang Renfei , Luo Fang , Ren Fu , Huang Wenli , Li Qianyi , Du Kaixuan , Yuan Dingdi
Published: February 2022

Urban wetlands provide cities with unique and valuable ecosystem services but are under great degradation pressure. Correctly identifying urban wetlands from remote sensing images is fundamental for developing appropriate management and protection plans. To overcome the semantic limitations of traditional pixel-level urban wetland classification techniques, we proposed an urban wetland identification framework based on an advanced scene-level classification scheme. First, the Sentinel-2 high-resolution multispectral image of Shenzhen was segmented into 320 m × 320 m square patches to generate sample datasets for classification. Next, twelve typical convolutional neural network (CNN) models were transformed for the comparison experiments. Finally, the model with the best performance was used to classify the wetland scenes in Shenzhen, and pattern and composition analyses were also implemented in the classification results. We found that the DenseNet121 model performed best in classifying urban wetland scenes, with overall accuracy (OA) and kappa values reaching 0.89 and 0.86, respectively. The analysis results revealed that the wetland scene in Shenzhen is generally balanced in the east–west direction. Among the wetland scenes, coastal open waters accounted for a relatively high proportion and showed an obvious southward pattern. The remaining swamp, marsh, tidal flat, and pond areas were scattered, accounting for only 4.64% of the total area of Shenzhen. For scattered and dynamic urban wetlands, we are the first to achieve scene-level classification with satisfactory results, thus providing a clearer and easier-to-understand reference for management and protection, which is of great significance for promoting harmony between humanity and ecosystems in cities.
IMGG: Integrating Multiple Single-Cell Datasets through Connected Graphs and Generative Adversarial Networks
Authors: Wang Xun , Zhang Chaogang , Zhang Ying , Meng Xiangyu , Zhang Zhiyuan , Shi Xin , Song Tao
Published: February 2022

There is a strong need to eliminate batch-specific differences when integrating single-cell RNA-sequencing (scRNA-seq) datasets generated under different experimental conditions for downstream task analysis. Existing batch correction methods usually transform different batches of cells into one preselected “anchor” batch or a low-dimensional embedding space, and cannot take full advantage of useful information from multiple sources. We present a novel framework, called IMGG, i.e., integrating multiple single-cell datasets through connected graphs and generative adversarial networks (GAN) to eliminate nonbiological differences between different batches. Compared with current methods, IMGG shows excellent performance on a variety of evaluation metrics, and the IMGG-corrected gene expression data incorporate features from multiple batches, allowing for downstream tasks such as differential gene expression analysis.
Cytoskeletal Protein 4.1G Is Essential for the Primary Ciliogenesis and Osteoblast Differentiation in Bone Formation
Authors: Saito Masaki , Hirano Marina , Izumi Tomohiro , Mori Yu , Ito Kentaro , Saitoh Yurika , Terada Nobuo , Sato Takeya , Sukegawa Jun
Published: February 2022

The primary cilium is a hair-like immotile organelle with specific membrane receptors, including the receptor of Hedgehog signaling, smoothened. The cilium organized in preosteoblasts promotes differentiation of the cells into osteoblasts (osteoblast differentiation) by mediating Hedgehog signaling to achieve bone formation. Notably, 4.1G is a plasma membrane-associated cytoskeletal protein that plays essential roles in various tissues, including the peripheral nervous system, testis, and retina. However, its function in the bone remains unexplored. In this study, we identified 4.1G expression in the bone. We found that, in the 4.1G-knockout mice, calcium deposits and primary cilium formation were suppressed in the trabecular bone, which is preosteoblast-rich region of the newborn tibia, indicating that 4.1G is a prerequisite for osteoblast differentiation by organizing the primary cilia in preosteoblasts. Next, we found that the primary cilium was elongated in the differentiating mouse preosteoblast cell line MC3T3-E1, whereas the knockdown of 4.1G suppressed its elongation. Moreover, 4.1G-knockdown suppressed the induction of the cilia-mediated Hedgehog signaling and subsequent osteoblast differentiation. These results demonstrate a new regulatory mechanism of 4.1G in bone formation that promotes the primary ciliogenesis in the differentiating preosteoblasts and induction of cilia-mediated osteoblast differentiation, resulting in bone formation at the newborn stage.
Mechanisms Underlying the Expansion and Functional Maturation of β-Cells in Newborns: Impact of the Nutritional Environment
Authors: Jacovetti Cécile , Regazzi Romano
Published: February 2022

The functional maturation of insulin-secreting β-cells is initiated before birth and is completed in early postnatal life. This process has a critical impact on the acquisition of an adequate functional β-cell mass and on the capacity to meet and adapt to insulin needs later in life. Many cellular pathways playing a role in postnatal β-cell development have already been identified. However, single-cell transcriptomic and proteomic analyses continue to reveal new players contributing to the acquisition of β-cell identity. In this review, we provide an updated picture of the mechanisms governing postnatal β-cell mass expansion and the transition of insulin-secreting cells from an immature to a mature state. We then highlight the contribution of the environment to β-cell maturation and discuss the adverse impact of an in utero and neonatal environment characterized by calorie and fat overload or by protein deficiency and undernutrition. Inappropriate nutrition early in life constitutes a risk factor for developing diabetes in adulthood and can affect the β-cells of the offspring over two generations. A better understanding of these events occurring in the neonatal period will help developing better strategies to produce functional β-cells and to design novel therapeutic approaches for the prevention and treatment of diabetes.
Omega-3 Polyunsaturated Fatty Acids—Vascular and Cardiac Effects on the Cellular and Molecular Level (Narrative Review)
Authors: Drenjančević Ines , Pitha Jan
Published: February 2022

In the prevention and treatment of cardiovascular disease, in addition to the already proven effective treatment of dyslipidemia, hypertension and diabetes mellitus, omega-3 polyunsaturated fatty acids (n-3 PUFAs) are considered as substances with additive effects on cardiovascular health. N-3 PUFAs combine their indirect effects on metabolic, inflammatory and thrombogenic parameters with direct effects on the cellular level. Eicosapentaenoic acid (EPA) seems to be more efficient than docosahexaenoic acid (DHA) in the favorable mitigation of atherothrombosis due to its specific molecular properties. The inferred mechanism is a more favorable effect on the cell membrane. In addition, the anti-fibrotic effects of n-3 PUFA were described, with potential impacts on heart failure with a preserved ejection fraction. Furthermore, n-3 PUFA can modify ion channels, with a favorable impact on arrhythmias. However, despite recent evidence in the prevention of cardiovascular disease by a relatively high dose of icosapent ethyl (EPA derivative), there is still a paucity of data describing the exact mechanisms of n-3 PUFAs, including the role of their particular metabolites. The purpose of this review is to discuss the effects of n-3 PUFAs at several levels of the cardiovascular system, including controversies.
VenomPred: A Machine Learning Based Platform for Molecular Toxicity Predictions
Authors: Galati Salvatore , Di Stefano Miriana , Martinelli Elisa , Macchia Marco , Martinelli Adriano , Poli Giulio , Tuccinardi Tiziano
Published: February 2022

The use of in silico toxicity prediction methods plays an important role in the selection of lead compounds and in ADMET studies since in vitro and in vivo methods are often limited by ethics, time, budget and other resources. In this context, we present our new web tool VenomPred, a user-friendly platform for evaluating the potential mutagenic, hepatotoxic, carcinogenic and estrogenic effects of small molecules. VenomPred platform employs several in-house Machine Learning (ML) models developed with datasets derived from VEGA QSAR, a software that includes a comprehensive collection of different toxicity models and has been used as a reference for building and evaluating our ML models. The results showed that our models achieved equal or better performance than those obtained with the reference models included in VEGA QSAR. In order to improve the predictive performance of our platform, we adopted a consensus approach combining the results of different ML models, which was able to predict chemical toxicity better than the single models. This improved method was thus implemented in the VenomPred platform, a freely accessible webserver that takes the SMILES (Simplified Molecular-Input Line-Entry System) strings of the compounds as input and sends the prediction results providing a probability score about their potential toxicity.
miRNome Profiling and Functional Analysis Reveal Involvement of hsa-miR-1246 in Colon Adenoma-Carcinoma Transition by Targeting AXIN2 and CFTR
Authors: Lukosevicius Rokas , Juzenas Simonas , Salteniene Violeta , Kulokiene Ugne , Arstikyte Justina , Hemmrich-Stanisak Georg , Franke Andre , Link Alexander , Ruzgys Paulius , Satkauskas Saulius , Pauzas Henrikas , Latkauskas Tadas , Kiudelis Gediminas , Balaguer Francesc , Kupcinskas Juozas , Skieceviciene Jurgita
Published: February 2022

Regulatory changes occurring early in colorectal cancer development remain poorly investigated. Since the majority of cases develop from polyps in the adenoma-carcinoma transition, a search of early molecular features, such as aberrations in miRNA expression occurring prior to cancer development, would enable identification of potentially causal, rather than consequential, candidates in the progression of polyp to cancer. In the current study, by employing small RNA-seq profiling of colon biopsy samples, we described differentially expressed miRNAs and their isoforms in the adenoma-carcinoma transition. Analysis of healthy-adenoma-carcinoma sequence in an independent validation group enabled us to identify early deregulated miRNAs including hsa-miR-1246 and hsa-miR-215-5p, the expressions of which are, respectively, gradually increasing and decreasing. Loss-of-function experiments revealed that inhibition of hsa-miR-1246 lead to reduced cell viability, colony formation, and migration rate, thereby indicating an oncogenic effect of this miRNA in vitro. Subsequent western blot and luciferase reporter assay provided evidence of hsa-miR-1246 being involved in the regulation of target AXIN2 and CFTR genes’ expression. To conclude, the present study revealed possible involvement of hsa-miR-1246 in early colorectal cancer development and regulation of tumor suppressors AXIN2 and CFTR.
Genome-Wide Analysis of the ATP-Binding Cassette (ABC) Transporter Family in Zea mays L. and Its Response to Heavy Metal Stresses
Authors: Guo Zhaolai , Yuan Xinqi , Li Linyang , Zeng Ming , Yang Jie , Tang Hong , Duan Changqun
Published: February 2022

The ATP-binding cassette (ABC) transporter family is one of the largest eukaryotic protein families. Its members play roles in numerous metabolic processes in plants by releasing energy for substrate transport across membranes through hydrolysis of ATP. Maize belongs to the monocotyledonous plant family, Gramineae, and is one of the most important food crops in the world. We constructed a phylogenetic tree with individual ABC genes from maize, rice, sorghum, Arabidopsis, and poplar. This revealed eight families, each containing ABC genes from both monocotyledonous and dicotyledonous plants, indicating that the amplification events of ABC gene families predate the divergence of plant monocotyledons. To further understand the functions of ABC genes in maize growth and development, we analyzed the expression patterns of maize ABC family genes in eight tissues and organs based on the transcriptome database on the Genevestigator website. We identified 133 ABC genes expressed in most of the eight tissues and organs examined, especially during root and leaf development. Furthermore, transcriptome analysis of ZmABC genes showed that exposure to metallic lead induced differential expression of many maize ABC genes, mainly including ZmABC 012, 013, 015, 031, 040, 043, 065, 078, 080, 085, 088, 102, 107, 111, 130 and 131 genes, etc. These results indicated that ZmABC genes play an important role in the response to heavy metal stress. The comprehensive analysis of this study provides a foundation for further studies into the roles of ABC genes in maize.
Biochemical Characterization of New Gemifloxacin Schiff Base (GMFX-o-phdn) Metal Complexes and Evaluation of Their Antimicrobial Activity against Some Phyto- or Human Pathogens
Authors: Elshafie Hazem S. , Sadeek Sadeek A. , Camele Ippolito , Mohamed Amira A.
Published: February 2022

Four novel ligand-metal complexes were synthesized through the reaction of Fe(III), pleaseCo(II), Zn(II), and Zr(IV) with Schiff base gemifloxacin reacted with ortho-phenylenediamine (GMFX-o-phdn) to investigate their biological activities. Elemental analysis, FT-IR, 1H NMR, UV-visible, molar conductance, melting points, magnetic susceptibility, and thermal analyses have been carried out for insuring the chelation process. The antimicrobial activity was carried out against Monilinia fructicola, Aspergillus flavus, Penicillium italicum, Botrytis cinerea, Escherichia coli, Bacillus cereus, Pseudomonas fluorescens, and P. aeruginosa. The radical scavenging activity (RSA%) was in vitro evaluated using ABTS method. FT-IR spectra indicated that GMFX-o-phdn chelated with metal ions as a tetradentate through oxygen of carboxylate group and nitrogen of azomethine group. The data of infrared, 1H NMR, and molar conductivity indicate that GMFX–o-phdn reacted as neutral tetra dentate ligand (N2O2) with metal ions through the two oxygen atoms of the carboxylic group (oxygen containing negative charge) and two nitrogen atoms of azomethine group (each nitrogen containing a lone pair of electrons) (the absent of peak corresponding to ν(COOH) at 1715 cm−1, the shift of azomethine group peak from 1633 cm−1 to around 1570 cm−1, the signal at 11 ppm of COOH and the presence of the chloride ions outside the complex sphere). Thermal analyses (TG-DTG/DTA) exhibited that the decaying of the metal complexes exists in three steps with the final residue metal oxide. The obtained data from DTA curves reflect that the degradation processes were exothermic or endothermic. Results showed that some of the studied complexes exhibited promising antifungal activity against most of the tested fungal pathogens, whereas they showed higher antibacterial activity against E. coli and B. cereus and low activity against P. fluorescens and P. aeruginosa. In addition, GMFX-o-phdn and its metal complexes showed strong antioxidant effect. In particular, the parent ligand and Fe(III) complex showed greater antioxidant capacity at low tested concentrations than that of other metal complexes where their IC50 were 169.7 and 164.6 µg/mL, respectively.
Dynamics of Ultrafast Phase Transitions in (001) Si on the Shock-Wave Front
Authors: Mareev Evgenii Igorevich , Potemkin Fedor Viktorovich
Published: February 2022

We demonstrate an ultrafast (<0.1 ps) reversible phase transition in silicon (Si) under ultrafast pressure loading using molecular dynamics. Si changes its structure from cubic diamond to β-Sn on the shock-wave front. The phase transition occurs when the shock-wave pressure exceeds 11 GPa. Atomic volume, centrosymmetry, and the X-ray-diffraction spectrum were revealed as effective indicators of phase-transition dynamics. The latter, being registered in actual experimental conditions, constitutes a breakthrough in the path towards simple X-ray optical cross-correlation and pump-probe experiments.
Actin-Associated Proteins and Small Molecules Targeting the Actin Cytoskeleton
Authors: Gao Jing , Nakamura Fumihiko
Published: February 2022

Actin-associated proteins (AAPs) act on monomeric globular actin (G-actin) and polymerized filamentous actin (F-actin) to regulate their dynamics and architectures which ultimately control cell movement, shape change, division; organelle localization and trafficking. Actin-binding proteins (ABPs) are a subset of AAPs. Since actin was discovered as a myosin-activating protein (hence named actin) in 1942, the protein has also been found to be expressed in non-muscle cells, and numerous AAPs continue to be discovered. This review article lists all of the AAPs discovered so far while also allowing readers to sort the list based on the names, sizes, functions, related human diseases, and the dates of discovery. The list also contains links to the UniProt and Protein Atlas databases for accessing further, related details such as protein structures, associated proteins, subcellular localization, the expression levels in cells and tissues, mutations, and pathology. Because the actin cytoskeleton is involved in many pathological processes such as tumorigenesis, invasion, and developmental diseases, small molecules that target actin and AAPs which hold potential to treat these diseases are also listed.
New-Born Screening for Spinal Muscular Atrophy: Results of a Latvian Pilot Study
Authors: Gailite Linda , Sterna Olga , Konika Maija , Isakovs Aleksejs , Isakova Jekaterina , Micule Ieva , Setlere Signe , Diriks Mikus , Auzenbaha Madara
Published: February 2022

New disease-modifying treatments have recently been approved for 5q spinal muscular atrophy (SMA) and early treatment has been associated with a better clinical outcome. Accordingly, new-born screening (NBS) for SMA should be implemented to ensure early diagnosis of affected individuals. The aim of this study was to determine the feasibility and usefulness of NBS for SMA in Latvia. Between February and November of 2021, 10,411 parents consented to participation in the study. DNA testing for the SMN1 exon 7 homozygous deletion was conducted using qPCR with fluorescent locked nucleic acid primers. In the first month of testing, reporting of results took up to a maximum of 17 days after samples arrived in the laboratory. However, following familiarisation with the procedure, the median report time was reduced to 11 days after birth. Forty cases required samples to be taken again due to poor quality of the isolated DNA transpiring from either the quality of the blood punch or manual mistakes during DNA isolation. The SMN1 exon 7 homozygous deletion was identified in two individuals, which was subsequently confirmed by multiplex ligation-dependent probe amplification. When a NBS sample is taken 48 to 72 h after birth and transported to the laboratory within two working days after collection according to legal requirements, DNA test results can be reported to healthcare professionals before the 12th day of life. Expansion of our SMA 5q NBS procedure to the whole of Latvia is feasible and would facilitate early diagnosis and result in more effective treatment. We strongly advocate that SMA is added to the national Latvia Recommended Uniform Screening Panel.
The Influence of Network Public Opinion on Audit Credibility: A Dynamic Rumor Propagation Model Based on User Weight
Authors: Zhu Lin , Li Jinyu , Bai Luyi
Published: February 2022

Network public opinion is one of the factors that affects the credibility of audits, especially falsified network public opinion, which can easily result in the public losing trust in audits and may even impact the financial market. As users of social networks are not online 24 h a day, and their network behaviors are dynamic, in this study, we constructed a dynamic rumor-spreading model. Because the influence and authority of different user nodes in the network are different, we added user weights to the rumor propagation model, and finally, we established a dynamic rumor propagation model based on user weights. The experimental results showed that the rumor propagation model had a good monitoring effect, so it could help with managing the public opinion of audit institutions, maintaining the image of audit fairness and justice, and maintaining the stability of the capital market.