bluuebunny/crossref_metadata_2025 huggingface dataset:
FAS receptor regulates NOTCH activity through ERK-JAG1 axis activation and controls oral cancer stemness ability and pulmonary metastasis
Authors: Li Li-Jie , Chang Peter Mu-Hsin , Li Chien-Hsiu , Chang Yu-Chan , Lai Tsung-Ching , Su Chia-Yi , Chen Chi-Long , Chang Wei-Min , Hsiao Michael , Feng Sheng-Wei
Published: March 2022

Pulmonary metastasis occurring via the colonization of circulating cancer stem cells is a major cause of oral squamous cell carcinoma (OSCC)-related death. Thus, understanding the mechanism of OSCC pulmonary metastasis may provide a new opportunity for OSCC treatment. FAS, a well-known apoptosis-inducing death receptor, has multiple nonapoptotic, protumorigenic functions. Previously, we found that SAS OSCC cells with FAS receptor knockout did not affect orthotopic tumor growth or cervical lymph node metastasis. However, FAS knockout cells could not colonize in distant organs to form metastases upon intravenous injection, which hinted at the cancer stemness function of the FAS receptor. Immunohistochemistry staining indicated that the FAS receptor serves as a poor prognosis marker in OSCC patients. FAS knockout inhibited in vitro cancer spheroid formation, migration and invasion, and prevented mesenchymal transition in OSCC cells and inhibited OSCC pulmonary metastasis in vivo. To determine the regulatory mechanism by which the FAS receptor exerts its oncogenic function, we utilized cDNA microarrays and phosphoprotein arrays to discover key candidate genes and signaling pathway regulators. JAG1 expression and NOTCH pathway activation were controlled by the FAS receptor through ERK phosphorylation. Both JAG1 and NOTCH1 silencing decreased in vitro cancer spheroid formation. In OSCC cells, FAS ligand or JAG1 protein treatment increased NOTCH pathway activity, which could be abolished by FAS receptor knockout. In FAS knockout cells, restoring the NOTCH1 intracellular domain stimulated cancer spheroid formation. Both JAG1 and NOTCH1 silencing decreased in vivo OSCC growth. In conclusion, we found a novel FAS-ERK-JAG1-NOTCH1 axis that may contribute to OSCC stemness and pulmonary metastasis.
The NSUN5-FTH1/FTL pathway mediates ferroptosis in bone marrow-derived mesenchymal stem cells
Authors: Liu Jie , Ren Zhenxing , Yang Lin , Zhu Lulu , li Yi , Bie Caiqun , Liu Helu , Ji Yichun , Chen Dongfeng , Zhu Meiling , Kuang Weihong
Published: March 2022

Ferroptosis is a type of cell death induced by the iron-dependent accumulation of lipid hydroperoxides and reactive oxygen species (ROS) in cells. Inhibiting ferroptosis is important for improving the survival of transplanted bone marrow-derived mesenchymal stem cells (BMSCs). Although it is known that NOP2/Sun RNA methyltransferase 5 (NSUN5) post-transcriptionally regulates ferroptosis in BMSCs through RNA methylation, the precise mechanisms underlying these effects have not been reported. In this study, we demonstrate that NSUN5 is downregulated in erastin-induced ferroptosis in BMSCs. Ferroptosis was inhibited by the overexpression ofNSUN5or ferritin heavy chain/light-chain (FTH1/FTL) and was enhanced byNSUN5knockdown. RNA immunoprecipitation experiments revealed that NSUN5 binds to FTH1/FTL, whileNSUN5depletion reduced the levels of 5-methylcytosine inFTH1/FTLRNA and increased intracellular iron concentrations, resulting in the downregulation of glutathione peroxidase 4 (GPX4) and the accumulation of ROS and lipid peroxidation products. Co-immunoprecipitation experiments demonstrated that the recognition ofFTH1andFTLby NSUN5 is dependent on the recruitment of tumor necrosis factor receptor-associated protein 1 (TRAP1). These results suggested that the NSUN5-FTH1/FTL pathway mediates ferroptosis in BMSCs and that the therapeutic targeting of components of this pathway may promote resistance to ferroptosis and improve the survival of transplanted BMSCs.
Mechanosensory trichome cells evoke a mechanical stimuli–induced immune response in Arabidopsis thaliana
Authors: Matsumura Mamoru , Nomoto Mika , Itaya Tomotaka , Aratani Yuri , Iwamoto Mizuki , Matsuura Takakazu , Hayashi Yuki , Mori Tsuyoshi , Skelly Michael J. , Yamamoto Yoshiharu Y. , Kinoshita Toshinori , Mori Izumi C. , Suzuki Takamasa , Betsuyaku Shigeyuki , Spoel Steven H. , Toyota Masatsugu , Tada Yasuomi
Published: March 2022

Perception of pathogen-derived ligands by corresponding host receptors is a pivotal strategy in eukaryotic innate immunity. In plants, this is complemented by circadian anticipation of infection timing, promoting basal resistance even in the absence of pathogen threat. Here, we report that trichomes, hair-like structures on the epidermis, directly sense external mechanical forces, including raindrops, to anticipate pathogen infections in Arabidopsis thaliana. Exposure of leaf surfaces to mechanical stimuli initiates the concentric propagation of intercellular calcium waves away from trichomes to induce defence-related genes. Propagating calcium waves enable effective immunity against pathogenic microbes through the CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR 3 (CAMTA3) and mitogen-activated protein kinases. We propose an early layer of plant immunity in which trichomes function as mechanosensory cells that detect potential risks.
Structural snapshots of V/A-ATPase reveal the rotary catalytic mechanism of rotary ATPases
Authors: Kishikawa J. , Nakanishi A. , Nakano A. , Saeki S. , Furuta A. , Kato T. , Mistuoka K. , Yokoyama K.
Published: March 2022

V/A-ATPase is a motor protein that shares a common rotary catalytic mechanism with FoF1 ATP synthase. When powered by ATP hydrolysis, the V1 domain rotates the central rotor against the A3B3 hexamer, composed of three catalytic AB dimers adopting different conformations (ABopen, ABsemi, and ABclosed). Here, we report the atomic models of 18 catalytic intermediates of the V1 domain of V/A-ATPase under different reaction conditions, determined by single particle cryo-EM. The models reveal that the rotor does not rotate immediately after binding of ATP to the V1. Instead, three events proceed simultaneously with the 120˚ rotation of the shaft: hydrolysis of ATP in ABsemi, zipper movement in ABopen by the binding ATP, and unzipper movement in ABclosed with release of both ADP and Pi. This indicates the unidirectional rotation of V/A-ATPase by a ratchet-like mechanism owing to ATP hydrolysis in ABsemi, rather than the power stroke model proposed previously for F1-ATPase.
Mental health in individuals with severe mental disorders during the covid-19 pandemic: a longitudinal investigation
Authors: Hofer Alex , Kachel Timo , Plattner Barbara , Chernova Anna , Conca Andreas , Fronthaler Martin , Haring Christian , Holzner Bernhard , Huber Markus , Marksteiner Josef , Miller Carl , Pardeller Silvia , Perwanger Verena , Pycha Roger , Schmidt Martin , Sperner-Unterweger Barbara , Tutzer Franziska , Frajo-Apor Beatrice
Published: March 2022

Research on the long-term mental health impact of the COVID-19 pandemic across mental disorders is limited, and information on the impact of public health policy measures with varying strictness is missing. This study therefore aimed at investigating psychological distress among residents of Tyrol (Austria) and South Tyrol (Italy) at the early stages of the pandemic and 5 months thereafter and examined how sociodemographic, protective, and risk factors relate to change over time. One hundred and fifteen people with severe mental illness (SMI; schizophrenia spectrum disorder, bipolar disorder, major depressive disorder with psychotic features) or major depressive disorder without psychotic features (MDD) and 481 community controls without mental disorders participated in an online survey. Next to the collection of sociodemographic and COVID-19 related variables, the Brief Symptom Checklist, the Resilience Scale, the Multidimensional Scale of Perceived Social Support, the Three-Item Loneliness Scale, and the Multidimensional State Boredom Scale-Short Form were used to assess psychological distress, resilience, perceived social support, loneliness, and boredom. Levels of psychological symptoms and the prevalence of psychological distress were significantly higher in individuals with MDD compared to the other two groups, and Italian participants were more prone to anxiety than those from Austria. Psychological distress was predicted by a lower degree of both resilience and perceived social support as well as loneliness and boredom. Notably, the prevalence of clinically relevant psychological symptoms remained unchanged among each group over time. These results underscore the relevance of tailored prevention and mitigation strategies to meet the specific needs of people both with and without mental disorders.
Exploring racial and gender disparities in voice biometrics
Authors: Chen Xingyu , Li Zhengxiong , Setlur Srirangaraj , Xu Wenyao
Published: March 2022

Systemic inequity in biometrics systems based on racial and gender disparities has received a lot of attention recently. These disparities have been explored in existing biometrics systems such as facial biometrics (identifying individuals based on facial attributes). However, such ethical issues remain largely unexplored in voice biometric systems that are very popular and extensively used globally. Using a corpus of non-speech voice records featuring a diverse group of 300 speakers by race (75 each from White, Black, Asian, and Latinx subgroups) and gender (150 each from female and male subgroups), we explore and reveal that racial subgroup has a similar voice characteristic and gender subgroup has a significant different voice characteristic. Moreover, non-negligible racial and gender disparities exist in speaker identification accuracy by analyzing the performance of one commercial product and five research products. The average accuracy for Latinxs can be 12% lower than Whites (p < 0.05, 95% CI 1.58%, 14.15%) and can be significantly higher for female speakers than males (3.67% higher, p < 0.05, 95% CI 1.23%, 11.57%). We further discover that racial disparities primarily result from the neural network-based feature extraction within the voice biometric product and gender disparities primarily due to both voice inherent characteristic difference and neural network-based feature extraction. Finally, we point out strategies (e.g., feature extraction optimization) to incorporate fairness and inclusive consideration in biometrics technology.
The impact of magnetized cold plasma and its various properties in sensing applications
Authors: Zaky Zaky A. , Panda Abinash , Pukhrambam Puspa D. , Aly Arafa H.
Published: March 2022

These analyses present a novel magnetized cold plasma-based 1D photonic crystal structure for detecting the refractive index of various bio-analytes. The proposed structure is designed with two photonic crystals composed of an alternating layer of right-hand polarization and left-hand polarization of the magnetized cold plasma material with a central defect layer. Transmittance characteristics of the structure are studied by employing the well-known transfer matrix method. Various geometrical parameters such as electron density, external magnetic field, thickness of odd and even layers of the multilayers, thickness of the sample layer, and incident angle are judiciously optimized to attain the best sensitivity, figure of merit, quality factor, signal-to-noise ratio, detection range and limit of detection. Finally, a maximum sensitivity of 25 GHz/RIU is accomplished with the optimized value of structure parameters, which can be considered as a noteworthy sensing performance.
Identifying key differences between linear stochastic estimation and neural networks for fluid flow regressions
Authors: Nakamura Taichi , Fukami Kai , Fukagata Koji
Published: March 2022

Neural networks (NNs) and linear stochastic estimation (LSE) have widely been utilized as powerful tools for fluid-flow regressions. We investigate fundamental differences between them considering two canonical fluid-flow problems: (1) the estimation of high-order proper orthogonal decomposition coefficients from low-order their counterparts for a flow around a two-dimensional cylinder, and (2) the state estimation from wall characteristics in a turbulent channel flow. In the first problem, we compare the performance of LSE to that of a multi-layer perceptron (MLP). With the channel flow example, we capitalize on a convolutional neural network (CNN) as a nonlinear model which can handle high-dimensional fluid flows. For both cases, the nonlinear NNs outperform the linear methods thanks to nonlinear activation functions. We also perform error-curve analyses regarding the estimation error and the response of weights inside models. Our analysis visualizes the robustness against noisy perturbation on the error-curve domain while revealing the fundamental difference of the covered tools for fluid-flow regressions.
Reconstitution of prenyltransferase activity on nanodiscs by components of the rubber synthesis machinery of the Para rubber tree and guayule
Authors: Kuroiwa Fu , Nishino Akira , Mandal Yasuko , Honzawa Masataka , Suenaga-Hiromori Miki , Suzuki Kakeru , Takani Yukie , Miyagi-Inoue Yukino , Yamaguchi Haruhiko , Yamashita Satoshi , Takahashi Seiji , Tozawa Yuzuru
Published: March 2022

Natural rubber of the Para rubber tree (Hevea brasiliensis) is synthesized as a result of prenyltransferase activity. The proteins HRT1, HRT2, and HRBP have been identified as candidate components of the rubber biosynthetic machinery. To clarify the contribution of these proteins to prenyltransferase activity, we established a cell-free translation system for nanodisc-based protein reconstitution and measured the enzyme activity of the protein-nanodisc complexes. Co-expression of HRT1 and HRBP in the presence of nanodiscs yielded marked polyisoprene synthesis activity. By contrast, neither HRT1, HRT2, or HRBP alone nor a complex of HRT2 and HRBP manifested such activity. Similar analysis of guayule (Parthenium argentatum) proteins revealed that three HRT1 homologs (PaCPT1–3) manifested prenyltransferase activity only when co-expressed with PaCBP, the homolog of HRBP. Our results thus indicate that two heterologous subunits form the core prenyltransferase of the rubber biosynthetic machinery. A recently developed structure modeling program predicted the structure of such heterodimer complexes including HRT1/HRBP and PaCPT2/PaCBP. HRT and PaCPT proteins were also found to possess affinity for a lipid membrane in the absence of HRBP or PaCBP, and structure modeling implicated an amphipathic α-helical domain of HRT1 and PaCPT2 in membrane binding of these proteins.
Harvesting of flow current through implanted hydrophobic PTFE surface within silicone-pipe as liquid nanogenerator
Authors: Cheedarala Ravi Kumar , Song Jung Il
Published: March 2022

Harvesting of flow current through implanted hydrophobic surface within silicone pipe as liquid nanogenerators where Tap water (TW), and DI water (DIw) as liquid reservoirs to successfully convert induced mechanical energy into electrical energy. Here, we used a commercial PTFE film for the generation of a hydrophobic surface as a source of mechanical energy. The surface roughness of the hydrophobic surface is confirmed using atomic force microscopy, and contact angle analyses. The generation of power through the interaction of TW and DI with inbuilt PTFE in silicone tube is described. The higher output voltage (Voc), and short circuit currents (Isc) were attained through an interaction of TW and DIw with N-PTFE. The lower Voc, and Isc’s were produced when DI water interacts with N-PTFE electrode, whereas TW produced higher Voc and Isc’s, respectively, due to a lack of free mobile ions in DIw than TW. The TW-Sh-TENG and DIw-Sh-TENG are produced the maximum peak-to-peak Voc, and Isc of 29.5 V and 17.4 V and 3.7 μA, and 2.9 μA, respectively. Significant power output enhancement of ~ 300% from TW-Sh-TENG from DIw-N-TENG due to the formation of higher surface roughness and lead to the slipping of water droplets by super-hydrophobicity.
Variation in diet composition and its relation to gut microbiota in a passerine bird
Authors: Schmiedová Lucie , Tomášek Oldřich , Pinkasová Hana , Albrecht Tomáš , Kreisinger Jakub
Published: March 2022

Quality and quantity of food items consumed has a crucial effect on phenotypes. In addition to direct effects mediated by nutrient resources, an individual’s diet can also affect the phenotype indirectly by altering its gut microbiota, a potent modulator of physiological, immunity and cognitive functions. However, most of our knowledge of diet-microbiota interactions is based on mammalian species, whereas little is still known about these effects in other vertebrates. We developed a metabarcoding procedure based on cytochrome c oxidase I high-throughput amplicon sequencing and applied it to describe diet composition in breeding colonies of an insectivorous bird, the barn swallow (Hirundo rustica). To identify putative diet-microbiota associations, we integrated the resulting diet profiles with an existing dataset for faecal microbiota in the same individual. Consistent with previous studies based on macroscopic analysis of diet composition, we found that Diptera, Hemiptera, Coleoptera and Hymenoptera were the dominant dietary components in our population. We revealed pronounced variation in diet consumed during the breeding season, along with significant differences between nearby breeding colonies. In addition, we found no difference in diet composition between adults and juveniles. Finally, our data revealed a correlation between diet and faecal microbiota composition, even after statistical control for environmental factors affecting both diet and microbiota variation. Our study suggests that variation in diet induce slight but significant microbiota changes in a non-mammalian host relying on a narrow spectrum of items consumed.
Novel chalcone-derived pyrazoles as potential therapeutic agents for the treatment of non-small cell lung cancer
Authors: Maciejewska Natalia , Olszewski Mateusz , Jurasz Jakub , Serocki Marcin , Dzierzynska Maria , Cekala Katarzyna , Wieczerzak Ewa , Baginski Maciej
Published: March 2022

Lung cancer is considered to account for approximately one-fifth of all malignant tumor-related deaths worldwide and is therefore one of the most lethal malignancies. Pyrazole scaffold possesses a wide range of biological and pharmacological activities, which play important roles in medicinal chemistry. The present study reports the synthesis and in vitro biological characterization of nine pyrazoles derived from chalcones as potential anticancer agents for non-small cell lung cancer A-549, H226, and H460 cell lines. Most of the compounds efficiently inhibited the growth of all the tested cancer cell lines at micromolar concentrations. One of the most active compounds (PCH-1) was further evaluated for its effect on cell cycle distribution, apoptosis, migration, epithelial–mesenchymal transition, and oxidative stress. Furthermore, studies on the mechanism of action revealed that PCH-1 disrupts microtubule assembly, leading to cancer cell death. Molecular modeling studies confirmed the potent interaction of PCH-1 with the vinblastine binding site on tubulin. Overall, this study provides novel opportunities to identify anticancer agents in the pyrazole series.
Epidemic models characterize seizure propagation and the effects of epilepsy surgery in individualized brain networks based on MEG and invasive EEG recordings
Authors: Millán Ana P. , van Straaten Elisabeth C. W. , Stam Cornelis J. , Nissen Ida A. , Idema Sander , Baayen Johannes C. , Van Mieghem Piet , Hillebrand Arjan
Published: March 2022

Epilepsy surgery is the treatment of choice for drug-resistant epilepsy patients. However, seizure-freedom is currently achieved in only 2/3 of the patients after surgery. In this study we have developed an individualized computational model based on MEG brain networks to explore seizure propagation and the efficacy of different virtual resections. Eventually, the goal is to obtain individualized models to optimize resection strategy and outcome. We have modelled seizure propagation as an epidemic process using the susceptible-infected (SI) model on individual brain networks derived from presurgical MEG. We included 10 patients who had received epilepsy surgery and for whom the surgery outcome at least one year after surgery was known. The model parameters were tuned in in order to reproduce the patient-specific seizure propagation patterns as recorded with invasive EEG. We defined a personalized search algorithm that combined structural and dynamical information to find resections that maximally decreased seizure propagation for a given resection size. The optimal resection for each patient was defined as the smallest resection leading to at least a 90% reduction in seizure propagation. The individualized model reproduced the basic aspects of seizure propagation for 9 out of 10 patients when using the resection area as the origin of epidemic spreading, and for 10 out of 10 patients with an alternative definition of the seed region. We found that, for 7 patients, the optimal resection was smaller than the resection area, and for 4 patients we also found that a resection smaller than the resection area could lead to a 100% decrease in propagation. Moreover, for two cases these alternative resections included nodes outside the resection area. Epidemic spreading models fitted with patient specific data can capture the fundamental aspects of clinically observed seizure propagation, and can be used to test virtual resections in silico. Combined with optimization algorithms, smaller or alternative resection strategies, that are individually targeted for each patient, can be determined with the ultimate goal to improve surgery outcome. MEG-based networks can provide a good approximation of structural connectivity for computational models of seizure propagation, and facilitate their clinical use.
Randomness-based macroscopic Franson-type nonlocal correlation
Authors: Ham Byoung S.
Published: March 2022

Franson-type nonlocal correlation is related to Bell inequality violation tests and has been applied for quantum key distributions based on time bin methods. Using unbalanced Mach–Zehnder interferometers, Franson correlation measurements result in an interference fringe, while local measurements do not. Here, randomness-based macroscopic Franson-type correlation is presented using polarization-based two-mode coherent photons, where the quantum correlation is tested by a Hong-Ou-Mandel scheme. Coherent photons are used to investigate the wave properties of this correlation. Without contradicting the wave-particle duality of quantum mechanics, the proposed method provides fundamental understanding of the quantum nature and opens the door to deterministic quantum information science.
Real-time infection prediction with wearable physiological monitoring and AI to aid military workforce readiness during COVID-19
Authors: Conroy Bryan , Silva Ikaro , Mehraei Golbarg , Damiano Robert , Gross Brian , Salvati Emmanuele , Feng Ting , Schneider Jeffrey , Olson Niels , Rizzo Anne G. , Curtin Catherine M. , Frassica Joseph , McFarlane Daniel C.
Published: March 2022

Infectious threats, like the COVID-19 pandemic, hinder maintenance of a productive and healthy workforce. If subtle physiological changes precede overt illness, then proactive isolation and testing can reduce labor force impacts. This study hypothesized that an early infection warning service based on wearable physiological monitoring and predictive models created with machine learning could be developed and deployed. We developed a prototype tool, first deployed June 23, 2020, that delivered continuously updated scores of infection risk for SARS-CoV-2 through April 8, 2021. Data were acquired from 9381 United States Department of Defense (US DoD) personnel wearing Garmin and Oura devices, totaling 599,174 user-days of service and 201 million hours of data. There were 491 COVID-19 positive cases. A predictive algorithm identified infection before diagnostic testing with an AUC of 0.82. Barriers to implementation included adequate data capture (at least 48% data was needed) and delays in data transmission. We observe increased risk scores as early as 6 days prior to diagnostic testing (2.3 days average). This study showed feasibility of a real-time risk prediction score to minimize workforce impacts of infection.
Multi-trait association study identifies loci associated with tolerance of low phosphorus in Oryza sativa and its wild relatives
Authors: Anandan Annamalai , Nagireddy Ranjitkumar , Sabarinathan Selvaraj , Bhatta Bishal Binaya , Mahender Anumalla , Vinothkumar Murugapandiyan , Parameswaran Chidambaranathan , Panneerselvam Periyasamy , Subudhi Hatanath , Meher Jitendriya , Bose Lotan Kumar , Ali Jauhar
Published: March 2022

We studied variation in adaptive traits and genetic association to understand the low P responses, including the symbiotic association of arbuscular mycorrhizal (AM) fungal colonization inOryzaspecies (O. sativa,O. nivara,andO. rufipogon). In the present experiment, we performed the phenotypic variability of the morphometric and geometric traits for P deficiency tolerance and conducted the association studies in GLM and MLM methods. A positive association between the geometric trait of the top-view area and root traits suggested the possibility of exploring a non-destructive approach in screening genotypes under low P. The AMOVA revealed a higher proportion of variation among the individuals as they belonged to different species ofOryzaand the NM value was 2.0, indicating possible gene flow between populations. A sub-cluster with superior-performing accessions had a higher proportion of landraces (42.85%), andO. rufipogon(33.3%) was differentiated by fourPup1-specific markers. Association mapping identified seven notable markers (RM259, RM297, RM30, RM6966, RM242, RM184, and PAP1) and six potential genotypes (IC459373, Chakhao Aumbi, AC100219, AC100062, Sekri, and Kumbhi Phou), which will be helpful in the marker-assisted breeding to improve rice for P-deprived condition. In addition, total root surface area becomes a single major trait that helps in P uptake under deficit P up to 33% than mycorrhizal colonization. Further, the phenotypic analysis of the morphometric and geometric trait variations and their interactions provides excellent potential for selecting donors for improving P-use efficiency. The identified potential candidate genes and markers offered new insights into our understanding of the molecular and physiological mechanisms driving PUE and improving grain yield under low-P conditions.
SSR individual identification system construction and population genetics analysis for Chamaecyparis formosensis
Authors: Huang Chiun-Jr , Chu Fang-Hua , Huang Yi-Shiang , Tu Yu-Ching , Hung Yu-Mei , Tseng Yu-Hsin , Pu Chang-En , Hsu Cheng Te , Chao Chi-Hsiang , Chou Yu-Shyang , Liu Shau-Chian , You Ya Ting , Hsu Shuo-Yu , Hsieh Hsiang-Chih , Wang Chieh-Ting , Chen Chi-Tsong
Published: March 2022

Chamaecyparis formosensis is an endemic species of Taiwan, threatened from intensive use and illegal felling. An individual identification system for C. formosensis is required to provide scientific evidence for court use and deter illegal felling. In this study, 36 polymorphic simple sequence repeat markers were developed. By applying up to 28 non-linked of the developed markers, it is calculated that the cumulative random probability of identity (CPI) is as low as 1.652 × 10–12, and the identifiable population size is up to 60 million, which is greater than the known C. formosensis population size in Taiwan. Biogeographical analysis data show that C. formosensis from four geographic areas belong to the same genetic population, which can be further divided into three clusters: SY (Eastern Taiwan), HV and GW (Northwestern Taiwan), and MM (Southwestern Taiwan). The developed system was applied to assess the provenance of samples with 88.44% accuracy rate and therefore can serve as a prescreening tool to reduce the range required for comparison. The system developed in this study is a potential crime-fighting tool against illegal felling.
A novel single-cell RNA-sequencing approach and its applicability connecting genotype to phenotype in ageing disease
Authors: Shomroni Orr , Sitte Maren , Schmidt Julia , Parbin Sabnam , Ludewig Fabian , Yigit Gökhan , Zelarayan Laura Cecilia , Streckfuss-Bömeke Katrin , Wollnik Bernd , Salinas Gabriela
Published: March 2022

Single cell multi-omics analysis has the potential to yield a comprehensive understanding of the cellular events that underlie the basis of human diseases. The cardinal feature to access this information is the technology used for single-cell isolation, barcoding, and sequencing. Most currently used single-cell RNA-sequencing platforms have limitations in several areas including cell selection, documentation and library chemistry. In this study, we describe a novel high-throughput, full-length, single-cell RNA-sequencing approach that combines the CellenONE isolation and sorting system with the ICELL8 processing instrument. This method offers substantial improvements in single cell selection, documentation and capturing rate. Moreover, it allows the use of flexible chemistry for library preparations and the analysis of living or fixed cells, whole cells independent of sizing and morphology, as well as of nuclei. We applied this method to dermal fibroblasts derived from six patients with different segmental progeria syndromes and defined phenotype associated pathway signatures with variant associated expression modifiers. These results validate the applicability of our method to highlight genotype-expression relationships for molecular phenotyping of individual cells derived from human patients.
Associations of maternal gestational hypertension with high blood pressure and overweight/obesity in their adolescent offspring: a retrospective cohort study
Authors: Kuciene Renata , Dulskiene Virginija
Published: March 2022

Maternal hypertensive disorders during pregnancy may have an impact on fetal development and the health of the offspring in later life. The aim of the study was to evaluate the associations of maternal gestational hypertension (GH) with high blood pressure (HBP) (prehypertension/hypertension) and overweight/obesity in their adolescent offspring at the age of 12 to 15 years. We analyzed data of 4819 participants born in Kaunas city during 1995–1998 who were included in the study “Prevalence and Risk Factors of HBP in 12–15-Year-Old Lithuanian Children and Adolescents”. The diagnosis of maternal gestational hypertension was obtained from medical records. Associations of maternal GH with their offspring’s HBP and overweight/obesity in adolescence were assessed by multivariate logistic regression analysis. Among 4819 adolescents of 12–15 years of age, 25.7% had HBP, 12% had overweight, and 2.5% had obesity. Of 4819 mothers, 92.3% were normotensive during pregnancy, and 7.7% had GH. In the multivariate analysis after adjustment for age, sex, birth weight, adolescent BMI, and maternal pre/early pregnancy BMI, adolescent offspring born to mothers with GH had higher odds of prehypertension, hypertension, and prehypertension/hypertension (aOR 1.58; 95% CI 1.13–2.22; aOR 1.87; 95% CI 1.41–2.47; and aOR 1.76, 95% CI 1.39–2.24; respectively), compared to the offspring of normotensive mothers. After adjustment for age, sex, birth weight, and maternal pre/early pregnancy BMI, a significant association was found between maternal GH and the offspring’s overweight/obesity in adolescence (aOR 1.41; 95% CI 1.04–1.91). The findings of this study suggest that maternal GH is associated with an increased odds of HBP (prehypertension and hypertension, both separately and combined) and overweight/obesity in their offspring during adolescence.
Reduced levels of pulmonary surfactant in COVID-19 ARDS
Authors: Schousboe Peter , Ronit Andreas , Nielsen Henning B. , Benfield Thomas , Wiese Lothar , Scoutaris Nikolaos , Verder Henrik , Berg Ronan M. G. , Verder Povl , Plovsing Ronni R.
Published: March 2022

To provide novel data on surfactant levels in adult COVID-19 patients, we collected bronchoalveolar lavage fluid less than 72 h after intubation and used Fourier Transform Infrared Spectroscopy to measure levels of dipalmitoylphosphatidylcholine (DPPC). A total of eleven COVID-19 patients with moderate-to-severe ARDS (CARDS) and 15 healthy controls were included. CARDS patients had lower DPPC levels than healthy controls. Moreover, a principal component analysis was able to separate patient groups into distinguishable subgroups. Our findings indicate markedly impaired pulmonary surfactant levels in COVID-19 patients, justifying further studies and clinical trials of exogenous surfactant.
Functional representativeness and distinctiveness of reintroduced birds and mammals in Europe
Authors: Thévenin Charles , Mouchet Maud , Robert Alexandre , Kerbiriou Christian , Sarrazin François
Published: March 2022

Reintroduction, the human-mediated movement of organisms to re-establish locally extinct populations, has become a popular conservation tool. However, because reintroductions often focus on local or national conservation issues, their contribution to the conservation of biodiversity at large scale remains unclear. While taxonomic biases have already been identified in reintroduction programs at regional scales, studies have stressed the need to account for other facets of biodiversity when assessing the relevance of the allocation of conservation efforts. In particular, it may be very fruitful to discriminate if and how such taxonomic biases may influence the functional complementarity of reintroduction targets, and to which extent reintroduction practitioners may have focused on species performing more singular functions than others. Here, we investigate the diversity of functional traits supported by reintroduced species of terrestrial birds and mammals in Europe. For each taxonomic group, we explored the functional representativeness of reintroduction targets at the European scale, i.e., whether species involved in reintroduction programs collectively represent the range of functional trait variation observed in the regional assemblage. Because additional conservation value could have been given by practitioners to species performing singular functions, we also measured the functional distinctiveness of reintroduced species. We found that reintroductions of birds did not focus on functionally distinct species, and that the subset of reintroduced birds is representative of the functional diversity at a continental scale. However, reintroductions of mammals involved more functionally distinct species than expected, even though reintroduced mammals are not collectively representative of the functional diversity of the continental assemblage.
Oral cancer induced TRPV1 sensitization is mediated by PAR2 signaling in primary afferent neurons innervating the cancer microenvironment
Authors: Scheff Nicole N. , Wall Ian M. , Nicholson Sam , Williams Hannah , Chen Elyssa , Tu Nguyen H. , Dolan John C. , Liu Cheng Z. , Janal Malvin N. , Bunnett Nigel W. , Schmidt Brian L.
Published: March 2022

Oral cancer patients report sensitivity to spicy foods and liquids. The mechanism responsible for chemosensitivity induced by oral cancer is not known. We simulate oral cancer-induced chemosensitivity in a xenograft oral cancer mouse model using two-bottle choice drinking and conditioned place aversion assays. An anatomic basis of chemosensitivity is shown in increased expression of TRPV1 in anatomically relevant trigeminal ganglion (TG) neurons in both the xenograft and a carcinogen (4-nitroquinoline 1-oxide)-induced oral cancer mouse models. The percent of retrograde labeled TG neurons that respond to TRPV1 agonist, capsaicin, is increased along with the magnitude of response as measured by calcium influx, in neurons from the cancer models. To address the possible mechanism of TRPV1 sensitivity in tongue afferents, we study the role of PAR2, which can sensitize the TRPV1 channel. We show co-expression of TRPV1 and PAR2 on tongue afferents and using a conditioned place aversion assay, demonstrate that PAR2 mediates oral cancer-induced, TRPV1-evoked sensitivity in an oral cancer mouse model. The findings provide insight into oral cancer-mediated chemosensitivity.
Sphk2 deletion is involved in structural abnormalities and Th17 response but does not aggravate colon inflammation induced by sub-chronic stress
Authors: Martín-Hernández David , Gutiérrez Irene L. , González-Prieto Marta , MacDowell Karina S. , Robledo-Montaña Javier , Tendilla-Beltrán Hiram , Calleja-Rodríguez Natalia , Bris Álvaro G. , Ulecia-Morón Cristina , Moreno Beatriz , Caso Javier R. , García-Bueno Borja , Rodrigues-Mascarenhas Sandra , Marín-Jiménez Ignacio , Leza Juan Carlos , Menchén Luis
Published: March 2022

The chronic inflammatory process that characterizes inflammatory bowel diseases (IBD) is mainly driven by T-cell response to microbial and environmental antigens. Psychological stress is a potential trigger of clinical flares of IBD, and sphingosine-1-phosphate (S1P) is involved in T-cell recruitment. Hence, stress impact and the absence of sphingosine kinase 2 (Sphk2), an enzyme of S1P metabolism, were evaluated in the colon of mice after sub-chronic stress exposure. Here, we show that sub-chronic stress increased S1P in the mouse colon, possibly due to a decrease in its degradation enzymes and Sphk2. S1P accumulation could lead to inflammation and immune dysregulation reflected by upregulation of toll-like receptor 4 (TLR4) pathway, inhibition of anti-inflammatory mechanisms, cytokine-expression profile towards a T-helper lymphocyte 17 (Th17) polarization, plasmacytosis, decrease in IgA+ lymphoid lineage cells (CD45+)/B cells/plasmablasts, and increase in IgM+ B cells. Stress also enhanced intestinal permeability. Sphk2 knockout mice presented a cytokine-expression profile towards a boosted Th17 response, lower expression of claudin 3,4,7,8, and structural abnormalities in the colon. Intestinal pathophysiology should consider stress and S1P as modulators of the immune response. S1P-based drugs, including Sphk2 potentiation, represent a promising approach to treat IBD.
Cervical spine immobilization does not interfere with nasotracheal intubation performed using GlideScope videolaryngoscopy: a randomized equivalence trial
Authors: Kuo Yi-Min , Lai Hsien-Yung , Tan Elise Chia-Hui , Li Yi-Shiuan , Chiang Ting-Yun , Huang Shiang-Suo , Huang Wen-Cheng , Chu Ya-Chun
Published: March 2022

GlideScope-assisted nasotracheal intubation (NTI) has been proposed as an alternative to difficult orotracheal intubation for critical patients or those under cervical immobilization. We evaluated the difficulty of performing NTI using GlideScope under cervical orthosis. A total of 170 patients scheduled for elective cervical spinal surgery that required NTI were randomized to receive cervical immobilization using a cervical collar (collar group) or no cervical immobilization at all (control group) before anesthetic induction (group assignment at 1:1 ratio). All NTI during anesthetic induction were performed using the GlideScope. The primary outcome was time to intubation. The secondary outcomes were ease of intubation, including the necessity of auxiliary manipulations to assist intubation, and the nasotracheal intubation difficulty scale (nasoIDS). An exploratory analysis identified morphometric parameters as predictors of time to intubation, the necessity of auxiliary manipulations, and a nasoIDS score ≥ 4. For time to intubation, the mean difference (collar group—control) was − 4.19 s, with a 95% confidence interval (CI) of − 13.9 to 5.52 that lay within our defined equivalence margin of 16 s. Multivariate regressions precluded the association of cervical immobilization with a necessity for auxiliary manipulations (adjusted odds ratio [aOR] 0.53, 95% CI [0.26–1.09], P = 0.083) and a nasoIDS ≥ 4 (aOR 0.94 [0.84–1.05], P = 0.280). Among all morphometric parameters, the upper lip bite test class was predictive of a longer time to intubation (all analyses relative to class 1, 14 s longer for class 2, P = 0.032; 24 s longer for class 3, P = 0.070), increased necessity for auxiliary manipulation (aOR 2.29 [1.06–4.94], P = 0.036 for class 2; aOR 6.12 [1.04–39.94], P = 0.045 for class 3), and nasoIDS ≥ 4 (aOR 1.46 [1.14–1.89], P = 0.003 for class 3).The present study demonstrated that GlideScope achieved NTI in patients with or without cervical immobilization equivalently with respect to intubation time and ease.
Relationship of choroidal thickness and axial length with posterior vitreous detachment in patients with high myopia
Authors: Hanyuda Akiko , Torii Hidemasa , Hayashi Ken , Uchida Atsuro , Mori Kiwako , Yotsukura Erisa , Ogawa Mamoru , Negishi Kazuno , Kurihara Toshihide , Tsubota Kazuo
Published: March 2022

Although accumulating evidence suggests a higher prevalence of posterior vitreous detachment (PVD) in highly myopic eyes, the relation between ocular biometric features and PVD stages in such eyes remains unclear. Therefore, we enrolled 170 patients with high myopia (axial length ≥ 26.0 mm) to investigate the status of PVD regarding subfoveal choroidal thickness and axial length. Utilising swept-source optical coherence tomography, we classified the PVD status into five stages. The distribution of PVD grades increased as the choroidal thickness decreased and axial length increased (P < 0.01). On adjusting for age and sex, decreased choroidal thickness and increased axial length were associated with more advanced PVD stages: odds ratios with the highest vs. lowest groups were 0.31 (95% confidence interval [CI] 0.09–1.01; Ptrend = 0.009) for choroidal thickness and 5.16 (95% CI 1.34–19.80; Ptrend = 0.002) for axial length. The inverse association between choroidal thickness and PVD status seemed stronger in women than in men (Pinteraction = 0.05). In conclusion, we firstly observed a significant trend of decreased choroidal thickness, along with increased axial length, with increased grade of PVD, particularly among women with highly myopic eyes, suggesting that advanced morphological myopic changes contribute to PVD in middle-aged adults.
SCORPION is a stacking-based ensemble learning framework for accurate prediction of phage virion proteins
Authors: Ahmad Saeed , Charoenkwan Phasit , Quinn Julian M. W. , Moni Mohammad Ali , Hasan Md Mehedi , Lio’ Pietro , Shoombuatong Watshara
Published: March 2022

Fast and accurate identification of phage virion proteins (PVPs) would greatly aid facilitation of antibacterial drug discovery and development. Although, several research efforts based on machine learning (ML) methods have been made for in silico identification of PVPs, these methods have certain limitations. Therefore, in this study, we propose a new computational approach, termed SCORPION, (StaCking-based Predictior fOR Phage VIrion PrOteiNs), to accurately identify PVPs using only protein primary sequences. Specifically, we explored comprehensive 13 different feature descriptors from different aspects (i.e., compositional information, composition-transition-distribution information, position-specific information and physicochemical properties) with 10 popular ML algorithms to construct a pool of optimal baseline models. These optimal baseline models were then used to generate probabilistic features (PFs) and considered as a new feature vector. Finally, we utilized a two-step feature selection strategy to determine the optimal PF feature vector and used this feature vector to develop a stacked model (SCORPION). Both tenfold cross-validation and independent test results indicate that SCORPION achieves superior predictive performance than its constitute baseline models and existing methods. We anticipate SCORPION will serve as a useful tool for the cost-effective and large-scale screening of new PVPs. The source codes and datasets for this work are available for downloading in the GitHub repository (https://github.com/saeed344/SCORPION).
Cold tolerance strategies of the fall armyworm, Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)
Authors: Vatanparast Mohammad , Park Youngjin
Published: March 2022

The fall armyworm (FAW), Spodoptera frugiperda, is native to the tropical and subtropical areas of the American continent and is one of the world's most destructive insect pests and invaded Africa and spread to most of Asia in two years. Glycerol is generally used as a cryoprotectant for overwintering insects in cold areas. In many studies, the increase in glycerol as a main rapid cold hardening (RCH) factor and enhancing the supercooling point was revealed at low temperatures. There are two genes, including glycerol-3-phosphate dehydrogenase (GPDH) and glycerol kinase (GK), that were identified as being associated with the glycerol synthesis pathway. In this study, one GPDH and two GK sequences (GK1 and GK2) were extracted from FAW transcriptome analysis. RNA interference (RNAi) specific to GPDH or GK1 and GK2 exhibited a significant down-regulation at the mRNA level as well as a reduction in survival rate when the RNAi-treated of FAW larvae post a RCH treatment. Following a cold period, an increase in glycerol accumulation was detected utilizing high-pressure liquid chromatography and colorimetric analysis of glycerol quantity in RCH treated hemolymph of FAW larvae. This research suggests that GPDH and GK isozymes are linked to the production of a high quantity of glycerol as an RCH factor, and glycerol as main cryoprotectant plays an important role in survival throughout the cold period in this quarantine pest studied.
Lessons from nine urban areas using data to drive local sustainable development
Authors: Jain Garima , Espey Jessica
Published: March 2022

Cities are central to achieving the 2030 Agenda for Sustainable Development, yet many remain disengaged from the process. This paper examines nine city and subnational pilot projects conducted between 2015 and 2019, to assess the role of a data-based approach to governance in improving decision-making for sustainable development. We consider to what extent a data-based approach to governance can help cities in (a) localizing the sustainable development goals (SDGs); (b) integrating national and local datasets; (c) increasing openness of data and enabling better accountability for outcomes; and (d) encouraging innovation in data gathering. We find that data is considered a useful entry point for local sustainable development conversations and employing a focus on data management is welcomed by nearly all local governments, however, few localities explicitly focus on data to improve their sustainable development outcomes. Critically, despite the excitement around big data, most cities are unable to access them due to capacity challenges, data ownership, and privacy concerns.
Multi-walled carbon nanotubes improve nitrogen use efficiency and nutritional quality inBrassica campestris
Authors: Cai Shixin , Zhang Peng , Guo Zhiling , Jin Feng , Wang Jingyuan , Song Zijie , Nadezhda Tcyganova , Lynch Iseult , Dang Xiuli
Published: March 2022

Multi-walled carbon nanotubes (MWCNTs) added into soil can improve the enzyme activities related to nitrogen (N) metabolism in the soil and plant to promoteBrassica campestrisgrowth and nitrogen use efficiency under different N applications.
Purified diet versus whole food diet and the inconsistent results in studies using animal models
Authors: Zhang Linyu , Li Xin , Liu Xiangyan , Wang Xianglin , Li Xiaowen , Cheng Xianyu , Yan Sisi , Zhu Yuanyuan , Li Rongfang , Wen Lixin , Wang Ji
Published: March 2022

In animal models, purified diets (PDs) and whole food diets (WFDs) are used for different purposes.
Rashba spin splitting and anomalous spin textures in the bulk ferroelectric oxide perovskite KIO3
Authors: Sheoran Sajjan , Kumar Manish , Bhumla Preeti , Bhattacharya Saswata
Published: March 2022

Band splitting and their associated spin textures obtained using 4-bandk·pHamiltonian are consequences of orbital-degeneracy. These spin textures are anomalous and observed to be beyond the linear superposition of trivial Rashba and Dresselhaus effects.