bluuebunny/crossref_metadata_2025 huggingface dataset:
Case report: Isolated axillary lymph node metastasis in high-risk endometrial cancer
Authors: Chen Yi-Ju Amy , Oo Myoe , Xu Yiqing
Published: March 2024

Introduction

There are risks of developing distant metastases over time for both early- and advanced-stage endometrial cancer. Axillary lymph node metastasis as the first site of recurrence, whether isolated or non-isolated, is uncommon, and there are currently no established treatment guidelines for such cases. This study highlights four cases of recurrent endometrial cancer that manifested axillary lymph node metastasis, providing a comprehensive review of their distinctive clinical behavior and the treatment strategies employed.

Methods

We reviewed and compared four cases of recurrent endometrial cancer that developed axillary lymph node metastasis following adjuvant treatment. Patients’ perspectives were also discussed.

Results

All four patients had aggressive endometrial histology, including high-grade serous carcinoma and carcinosarcoma. The stages at presentation were stages I and III, with laparotomy or laparoscopy used as the initial surgical approach. Axillary lymph node metastasis was the primary site of recurrence in three cases. Of the three patients with isolated axillary lymph node metastasis, two had long-term survival after aggressive locoregional treatment comprising surgery and radiation.

Conclusion

Axillary lymph node metastasis as the first site of recurrence is rare, even in high-risk endometrial cancer. In addition to systemic chemotherapy, aggressive locoregional treatment can potentially maximize the chance of long-term disease control.
Prevention of cisplatin-induced hearing loss in children: achievements and challenges for evidence-based implementation of sodium Thiosulfate
Authors: Meijer Annelot J. M. , Diepstraten Franciscus A. , van den Heuvel-Eibrink Marry M. , Bleyer Archie
Published: March 2024

Ototoxicity is a devastating direct, irreversible side effect of platinum use in children with cancer, with its consequent effect on speech, language and social development, quality of life and adult productivity. Cisplatin, an essential chemotherapeutic agent for the treatment of solid tumors in children, is a DNA cross-linking agent. Which causes hearing loss in 50-70% of cisplatin treated children. Fortunately, to prevent hearing loss, sodium thiosulfate (STS), which binds to cisplatin, and reduces the superoxides in both tumor and outer hair cells of the cochlea has now been discovered to be an effective and safe otoprotectant if administered correctly. The aim of this perspective paper is to explore the key safety issues and challenges important for pediatric oncologists and pharmacists when considering the clinical use of STS as an otoprotectant for children and adolescents receiving cisplatin. These include: the choice of the formulation; the timing, both that of the STS in relation to cisplatin as well as the timing of the cisplatin infusion itself; the dosing; the challenge left by the definition of localized versus disseminated disease and the difference in indication for STS, between cisplatin treated patients and those receiving another platinum chemotherapeutic agent, carboplatin.
A deep insight into ferroptosis in lung disease: facts and perspectives
Authors: Zhang Fan , Xiang Yu , Ma Qiao , Guo E. , Zeng Xiansheng
Published: March 2024

In the last decade, ferroptosis has received much attention from the scientific research community. It differs from other modes of cell death at the morphological, biochemical, and genetic levels. Ferroptosis is mainly characterized by non-apoptotic iron-dependent cell death caused by iron-dependent lipid peroxide excess and is accompanied by abnormal iron metabolism and oxidative stress. In recent years, more and more studies have shown that ferroptosis is closely related to the occurrence and development of lung diseases. COPD, asthma, lung injury, lung fibrosis, lung cancer, lung infection and other respiratory diseases have become the third most common chronic diseases worldwide, bringing serious economic and psychological burden to people around the world. However, the exact mechanism by which ferroptosis is involved in the development and progression of lung diseases has not been fully revealed. In this manuscript, we describe the mechanism of ferroptosis, targeting of ferroptosis related signaling pathways and proteins, summarize the relationship between ferroptosis and respiratory diseases, and explore the intervention and targeted therapy of ferroptosis for respiratory diseases.
Isoquercitrin alleviates pirarubicin-induced cardiotoxicity in vivo and in vitro by inhibiting apoptosis through Phlpp1/AKT/Bcl-2 signaling pathway
Authors: Wang Lei , Ma Jiulong , Chen Chen , Lin Bin , Xie Sicong , Yang Weiwei , Qian Jiajia , Zhang Yang
Published: March 2024

Introduction: Due to the cardiotoxicity of pirarubicin (THP), it is necessary to investigate new compounds for the treatment of THP-induced cardiotoxicity. Isoquercitrin (IQC) is a natural flavonoid with anti-oxidant and anti-apoptosis properties. Thus, the present study aimed to investigate the influence of IQC on preventing the THP-induced cardiotoxicity in vivo and in vitro.

Methods: The optimal concentration and time required for IQC to prevent THP-induced cardiomyocyte damage were determined by an MTT assay. The protective effect was further verified in H9c2 and HCM cells using dichlorodihydrofluorescein diacetate fluorescent probes, MitoTracker Red probe, enzyme-linked immunosorbent assay, JC-1 probe, and real time-quantitative polymerase chain reaction (RT-qPCR). Rats were administered THP to establish cardiotoxicity. An electrocardiogram (ECG) was performed, and cardiac hemodynamics, myocardial enzymes, oxidative stress indicators, and hematoxylin-eosin staining were studied. Voltage-dependent anion channel 1 (VDAC1), adenine nucleotide translocase 1 (ANT1), and cyclophilin D (CYPD) were detected by qRT-PCR, and the Phlpp1/AKT/Bcl-2 axis proteins were detected by western blot, confirming that IQC markedly increased cell viability and superoxide dismutase (SOD) levels, diminished the levels of ROS and MDA, and elevated mitochondrial function and apoptosis in vivo and in vitro.

Results: Results showed that IQC reduced THP-induced myocardial histopathological injury, electrocardiogram (ECG) abnormalities, and cardiac dysfunction in vivo. IQC also decreased serum levels of MDA, BNP, CK-MB, c-TnT, and LDH, while increasing levels of SOD and GSH. We also found that IQC significantly reduced VDAC1, ANT1, and CYPD mRNA expression. In addition, IQC controlled apoptosis by modulating Phlpp1/AKT/Bcl-2 signaling pathways. IQC markedly increased H9c2 and HCM cell viability and SOD levels, diminished the levels of ROS and MDA, and elevated mitochondrial function in H9c2 and HCM cells to defend against THP-induced cardiomyocyte apoptosis in vitro. The AKT inhibitor IMQ demonstrated that IQC lacked antioxidant and anti-apoptotic properties. Moreover, our data showed that IQC regulates Phlpp1 expression, thereby influencing the expression levels of p-AKT, cytochrome c, caspase-3, caspase-9, Bcl-2, and Bax.

Discussion: In conclusion, our results indicate that IQC protects the changes in mitochondrial membrane permeability in cardiomyocytes by regulating the Phlpp1/AKT/Bcl-2 signaling pathway, inhibits the release of cytc from the mitochondrial inner membrane to the cytoplasm, forms apoptotic bodies, induces cell apoptosis, and reduces THP induced cardiotoxicity.
Examining the safety of mirabegron: an analysis of real-world pharmacovigilance data from the US FDA adverse event reporting system (FAERS) database
Authors: Wang Junwei , Zhang Aiwei , Ye Miaoyong , Zhang Cunming
Published: March 2024

Background: Mirabegron, the first β-3 adrenergic receptor agonist, received approval from the Food and Drug Administration (FDA) in 2012 for the treatment of overactive bladder (OAB). This pharmacovigilance study investigated the safety profile of mirabegron treatment using the US FDA Adverse Event Reporting System (FAERS) database.

Methods: This study employed disproportionality analyses, including the reporting odds ratio (ROR) and Bayesian Confidence Propagation Neural Network (BCPNN) algorithm, to quantify signals of adverse events associated with mirabegron.

Results: From the first quarter of 2012 to the third quarter of 2023, a comprehensive total of 14,356,234 adverse event (AE) reports were submitted to the FDA Adverse Event Reporting System database. Within this dataset, encompassing 18,763 reports specifically associated with mirabegron, healthcare professionals notably contributed 2,902 of these reports. A total of 80 preferred terms (PTs) of interest were identified using both the ROR and information component algorithms. The most common AEs included blood pressure increased, urinary retention, atrial fibrillation, dry mouth, and tachycardia, which were consistent with the product instructions. Unexpected significant AEs, such as arrhythmia, palpitations, dementia, transient ischemic attack, Parkinson’s disease, anti-neutrophil cytoplasmic antibody positive vasculitis, lip swelling, and swollen tongue, were also identified. The study findings indicated that the majority of onset time occurred within 30 days (n = 358, 55.68%). However, AEs were still possible after 1 year of mirabegron treatment.

Conclusion: This study provided valuable evidence for the real-world safety of mirabegron, helping clinical professionals enhance their understanding of mirabegron’s safety in clinical practice. It also contributed valuable evidence for further safety studies on mirabegron.
Electrical impedance tomography as a bedside assessment tool for COPD treatment during hospitalization
Authors: Yang Lin , Gao Zhijun , Cao Xinsheng , Sun Shuying , Wang Chunchen , Wang Hang , Dai Jing , Liu Yang , Qin Yilong , Dai Meng , Guo Wei , Zhang Binghua , Zhao Ke , Zhao Zhanqi
Published: March 2024

For patients with chronic obstructive pulmonary disease (COPD), the assessment of the treatment efficacy during hospitalization is of importance to the optimization of clinical treatments. Conventional spirometry might not be sensitive enough to capture the regional lung function development. The study aimed to evaluate the feasibility of using electrical impedance tomography (EIT) as an objective bedside evaluation tool for the treatment of acute exacerbation of COPD (AECOPD). Consecutive patients who required hospitalization due to AECOPD were included prospectively. EIT measurements were conducted at the time of admission and before the discharge simultaneously when a forced vital capacity maneuver was conducted. EIT-based heterogeneity measures of regional lung function were calculated based on the impedance changes over time. Surveys for attending doctors and patients were designed to evaluate the ease of use, feasibility, and overall satisfaction level to understand the acceptability of EIT measurements. Patient-reported outcome assessments were conducted. User’s acceptance of EIT technology was investigated with a five-dimension survey. A total of 32 patients were included, and 8 patients were excluded due to the FVC maneuver not meeting the ATS criteria. Spirometry-based lung function was improved during hospitalization but not significantly different (FEV1 %pred.: 35.8% ± 6.7% vs. 45.3% ± 8.8% at admission vs. discharge; p = 0.11. FVC %pred.: 67.8% ± 0.4% vs. 82.6% ± 5.0%; p = 0.15. FEV1/FVC: 0.41 ± 0.09 vs. 0.42 ± 0.07, p = 0.71). The symptoms of COPD were significantly improved, but the correlations between the improvement of symptoms and spirometry FEV1 and FEV1/FVC were low (R = 0.1 and −0.01, respectively). The differences in blood gasses and blood tests were insignificant. All but one EIT-based regional lung function parameter were significantly improved after hospitalization. The results highly correlated with the patient-reported outcome assessment (R > 0.6, p < 0.001). The overall acceptability score of EIT measurement for both attending physicians and patients was high (4.1 ± 0.8 for physicians, 4.5 ± 0.5 for patients out of 5). These results demonstrated that it was feasible and acceptable to use EIT as an objective bedside evaluation tool for COPD treatment efficacy.
Fibrosis modeling choice affects morphology of ventricular arrhythmia in non-ischemic cardiomyopathy
Authors: Myklebust Lena , Maleckar Mary M. , Arevalo Hermenegild
Published: March 2024

Introduction: Patients with non-ischemic cardiomyopathy (NICM) are at risk for ventricular arrhythmias, but diagnosis and treatment planning remain a serious clinical challenge. Although computational modeling has provided valuable insight into arrhythmic mechanisms, the optimal method for simulating reentry in NICM patients with structural disease is unknown.

Methods: Here, we compare the effects of fibrotic representation on both reentry initiation and reentry morphology in patient-specific cardiac models. We investigate models with heterogeneous networks of non-conducting structures (cleft models) and models where fibrosis is represented as a dense core with a surrounding border zone (non-cleft models). Using segmented cardiac magnetic resonance with late gadolinium enhancement (LGE) of five NICM patients, we created 185 3D ventricular electrophysiological models with different fibrotic representations (clefts, reduced conductivity and ionic remodeling).

Results: Reentry was induced by electrical pacing in 647 out of 3,145 simulations. Both cleft and non-cleft models can give rise to double-loop reentries meandering through fibrotic regions (Type 1-reentry). When accounting for fibrotic volume, the initiation sites of these reentries are associated with high local fibrotic density (mean LGE in cleft models: p< 0.001, core volume in non-cleft models: p = 0.018, negative binomial regression). In non-cleft models, Type 1-reentries required slow conduction in core tissue (non-cleftsc models) as opposed to total conduction block. Incorporating ionic remodeling in fibrotic regions can give rise to single- or double-loop rotors close to healthy-fibrotic interfaces (Type 2-reentry). Increasing the cleft density or core-to-border zone ratio in cleft and non-cleftc models, respectively, leads to increased inducibility and a change in reentry morphology from Type 2 to Type 1.

Conclusions: By demonstrating how fibrotic representation affects reentry morphology and location, our findings can aid model selection for simulating arrhythmogenesis in NICM.
The effect of perceived stress on binge eating behavior among Chinese university students: a moderated mediation model
Authors: Li Chunlu , Gu Jing , Li Yixin , Xia Baijuan , Meng Xiaolu
Published: March 2024

Introduction

Previous studies have demonstrated a strong link between perceived stress and binge eating behavior, but the psychological mechanisms underlying such phenomenon are not fully understood. The present study further addressed this issue in a life history framework, focusing on life history strategy and distress tolerance.

Methods

Firstly, we investigated the mediation role of life history strategy on the relationship between perceived stress and binge eating behavior. Secondly, we examined the moderation role of distress tolerance on the effect of perceived stress on life history strategy, as well as on the direct effect of perceived stress on binge eating behavior. We analyzed data from 1342 Chinese university students.

Results

Results indicated that life history strategy mediates the relationship between perceived stress and binge eating behavior; distress tolerance has significant moderating effects on the direct effect of perceived stress on binge eating behavior and their indirect effect via life history strategy.

Discussion

Therefore, distress tolerance skills training and life history-based interventions might be potentially effective ways to reduce binge eating behavior triggered by perceived stress.
An integrated hierarchical classification and machine learning approach for mapping land use and land cover in complex social-ecological systems
Authors: Ojwang Gordon O. , Ogutu Joseph O. , Said Mohammed Y. , Ojwala Merceline A. , Kifugo Shem C. , Verones Francesca , Graae Bente J. , Buitenwerf Robert , Olff Han
Published: March 2024

Mapping land use and land cover (LULC) using remote sensing is fundamental to environmental monitoring, spatial planning and characterising drivers of change in landscapes. We develop a new, general and versatile approach for mapping LULC in landscapes with relatively gradual transition between LULC categories such as African savannas. The approach integrates a well-tested hierarchical classification system with the computationally efficient random forest (RF) classifier and produces detailed, accurate and consistent classification of structural vegetation heterogeneity and density and anthropogenic land use. We use Landsat 8 OLI imagery to illustrate this approach for the Extended Greater Masai Mara Ecosystem (EGMME) in southwestern Kenya. We stratified the landscape into eight relatively homogeneous zones, systematically inspected the imagery and randomly allocated 1,697 training sites, 556 of which were ground-truthed, proportionately to the area of each zone. We directly assessed the accuracy of the visually classified image. Accuracy was high and averaged 88.1% (80.5%–91.7%) across all the zones and 89.1% (50%–100%) across all the classes. We applied the RF classifier to randomly selected samples from the original training dataset, separately for each zone and the EGMME. We evaluated the overall and class-specific accuracy and computational efficiency using the Out-of-Bag (OOB) error. Overall accuracy (79.3%–97.4%) varied across zones but was higher whereas the class-specific accuracy (25.4%–98.1%) was lower than that for the EGMME (80.2%). The hierarchical classifier identified 35 LULC classes which we aggregated into 18 intermediate mosaics and further into five more general categories. The open grassed shrubland (21.8%), sparse shrubbed grassland (10.4%) and small-scale cultivation (13.3%) dominated at the detailed level, grassed shrubland (31.9%) and shrubbed grassland (28.9%) at the intermediate level, and grassland (35.7%), shrubland (35.3%) and woodland (12.5%) at the general level. Our granular LULC map for the EGMME is sufficiently accurate for important practical purposes such as land use spatial planning, habitat suitability assessment and temporal change detection. The extensive ground-truthing data, sample site photos and classified maps can contribute to wider validation efforts at regional to global scales.
Q&A of liquid crystal alignment: theory and practice
Authors: Muravsky Alexander , Murauski Anatoli
Published: March 2024

We selected and summarized the most important aspects of liquid crystal alignment. Alignment by the surface profile and flat surface alignment are discussed as the main factors leading to the high-quality orientation of liquid crystals. The advanced level of understanding and formulation allows linking the alignment layer properties to the molecular structure of the alignment material. The paper reviews and combines the latest knowledge about liquid crystal alignment, dwells upon the relationship between the polar and azimuthal anchoring energy, explains the major particularities regarding anchoring measurements in the case of birefringent alignment materials with truly high azimuthal anchoring energy, and observes the current trends of water-friendly alignment materials and their application in geometrical phase device fabrication.
Exploring Virtual Environments to Assess the Quality of Public Spaces
Authors: Belaroussi Rachid , Issa Elie , Cameli Leonardo , Lantieri Claudio , Adelé Sonia
Published: March 2024

Human impression plays a crucial role in effectively designing infrastructures that support active mobility such as walking and cycling. By involving users early in the design process, valuable insights can be gathered before physical environments are constructed. This proactive approach enhances the attractiveness and safety of designed spaces for users. This study conducts an experiment comparing real street observations with immersive virtual reality (VR) visits to evaluate user perceptions and assess the quality of public spaces. For this experiment, a high-resolution 3D city model of a large-scale neighborhood was created, utilizing Building Information Modeling (BIM) and Geographic Information System (GIS) data. The model incorporated dynamic elements representing various urban environments: a public area with a tramway station, a commercial street with a road, and a residential playground with green spaces. Participants were presented with identical views of existing urban scenes, both in reality and through reconstructed 3D scenes using a Head-Mounted Display (HMD). They were asked questions related to the quality of the streetscape, its walkability, and cyclability. From the questionnaire, algorithms for assessing public spaces were computed, namely Sustainable Mobility Indicators (SUMI) and Pedestrian Level of Service (PLOS). The study quantifies the relevance of these indicators in a VR setup and correlates them with critical factors influencing the experience of using and spending time on a street. This research contributes to understanding the suitability of these algorithms in a VR environment for predicting the quality of future spaces before occupancy.
Development and Preliminary Validation of the Sexual Minority Identity Emotion Scale
Authors: Goffnett Jacob , Robinson Samantha , Hamaker Anna , Rahman Mohammod Mahmudur , Schrager Sheree M. , Goldbach Jeremy T.
Published: March 2024

Emotions influence health behaviors and outcomes, yet little research has examined the emotion–health relationship among sexual minorities. The few studies in this area have used general measures of feelings without regard for identity, despite the literature positing emotions as culturally and contextually specific. This critical limitation obscures inferences made in studies that have found emotions to predict mental health outcomes for sexual minorities. This study begins to address this gap by developing and examining the preliminary validation of the Sexual Minority Identity Emotion Scale, a measure of shame and pride specific to the identity experiences of sexual minority adolescents. The initial pool of items emerged from a qualitative study and was refined through a multistep review. The measurement’s factor structure and criterion validity were examined using a nationwide sample of 273 sexual minority adolescents from the United States. The scale has four factors with strong internal reliability, adequate criterion validity, and utility in health research.
Generation of Secondary Space Debris Risks from Net Capturing in Active Space Debris Removal Missions
Authors: Cuadrat-Grzybowski Michal , Gill Eberhard
Published: March 2024

Mitigation strategies to eliminate existing space debris, such as with Active Space Debris Removal (ASDR) missions, have become increasingly important. Among the considered ASDR approaches, one involves using a net as a capturing mechanism. A fundamental requirement for any ASDR mission is that the capture process itself should not give rise to new space debris. However, in simulations of net capturing, the potential for structural breaking is often overlooked. A discrete Multi-Spring-Damper net model was employed to simulate the impact of a 30 m × 30 m net travelling at 20 m/s onto an ESA Envisat mock-up. The Envisat was modelled as a two-rigid-body system comprised of the main body and a large solar array with a hinge connection. The analysis revealed that more than two significant substructures had a notable likelihood of breaking, prompting the recommendation of limiting the impacting velocity. The generation of secondary space debris indicates that net capturing is riskier than previously assumed in the literature.
An Apple Detection and Localization Method for Automated Harvesting under Adverse Light Conditions
Authors: Zhang Guoyu , Tian Ye , Yin Wenhan , Zheng Change
Published: March 2024

The use of automation technology in agriculture has become particularly important as global agriculture is challenged by labor shortages and efficiency gains. The automated process for harvesting apples, an important agricultural product, relies on efficient and accurate detection and localization technology to ensure the quality and quantity of production. Adverse lighting conditions can significantly reduce the accuracy of fruit detection and localization in automated apple harvesting. Based on deep-learning techniques, this study aims to develop an accurate fruit detection and localization method under adverse light conditions. This paper explores the LE-YOLO model for accurate and robust apple detection and localization. The traditional YOLOv5 network was enhanced by adding an image enhancement module and an attention mechanism. Additionally, the loss function was improved to enhance detection performance. Secondly, the enhanced network was integrated with a binocular camera to achieve precise apple localization even under adverse lighting conditions. This was accomplished by calculating the 3D coordinates of feature points using the binocular localization principle. Finally, detection and localization experiments were conducted on the established dataset of apples under adverse lighting conditions. The experimental results indicate that LE-YOLO achieves higher accuracy in detection and localization compared to other target detection models. This demonstrates that LE-YOLO is more competitive in apple detection and localization under adverse light conditions. Compared to traditional manual and general automated harvesting, our method enables automated work under various adverse light conditions, significantly improving harvesting efficiency, reducing labor costs, and providing a feasible solution for automation in the field of apple harvesting.
Analysis of the Physico-Chemical Properties of Bean Seeds after Three Years of Digestate Use
Authors: Koszel Milan , Parafiniuk Stanisław , Kocira Sławomir , Bochniak Andrzej , Przywara Artur , Lorencowicz Edmund , Findura Pavol , Atanasov Atanas Zdravkov
Published: March 2024

Taking into consideration its physico-chemical properties, digestate should be used primarily as a fertiliser. The possible ways of using digestate as a fertiliser in agriculture were identified, and digestate collected from an agricultural biogas plant was tested for its macroelement and heavy metal content. The research was conducted on Haplic LUVISOLS soil according FAO classification. The area of the land plots was 75 m2. All measurements were carried out in ten replicates. Seed yield was determined at 2.6 t ha−1. The thousand-seed weight was similar in the three growing seasons, and averaged 171.49 g to 184.44 g for the three years under analysis. For the control object, the average thousand-seed weight from the three years of the experiment was 168.56 g. This parameter was significantly influenced by the year of analysis. The highest protein content was obtained in 2022 (an average of 20.3%), which was significantly higher than in 2021 (20.13%) and 2020 (20.12%). The analysis showed an increase in the average value for the three harvest years regarding the fat content of the multiflora bean seeds depending on the post-harvest digestate dose, ranging from 0.47% to 0.61%. In the control object, the average fat content for the three harvest years under analysis was 0.41%. The year under analysis had no significant impact on fat content. A positive correlation was found between the digestate dose and protein, fat, and carbohydrate contents per 100 g of beans. Increasing the dose resulted in statistically significant differences from the lower dose. The obtained results show an increase in macroelement content depending on the digestate dose applied. The average carbohydrate content per 100 g of beans for the three years under analysis ranged from 49.78 g to 54.01 g, while the calcium content per 100 g of beans ranged from 109.23 mg to 124.00 mg. In contrast, the magnesium content in 100 g of bean ranged from 129.91 g to 137.01 mg, the phosphorus content in 100 g of bean from 366.99 mg to 387.00 mg, and the potassium content in 100 g of bean from 1341.20 mg to 1394.06 mg. Statistical analysis revealed statistically significant differences except for potassium, where no differences were found for the two highest doses. In addition, no differences were found in the average phosphorus and potassium content between the years under analysis. The study showed an increase in yield depending on the amount of digestate applied. The highest dose used in the experiment provided the most nitrogen and macronutrients, with a positive effect on yield velocity, protein and fat content, micronutrients, and macronutrients in beans.
An Integrated IoT Sensor-Camera System toward Leveraging Edge Computing for Smart Greenhouse Mushroom Cultivation
Authors: Nguyen Hoang Hai , Shin Dae-Yun , Jung Woo-Sung , Kim Tae-Yeol , Lee Dae-Hyun
Published: March 2024

Industrial greenhouse mushroom cultivation is currently promising, due to the nutritious and commercial mushroom benefits and its convenience in adapting smart agriculture technologies. Traditional Device-Cloud protocol in smart agriculture wastes network resources when big data from Internet of Things (IoT) devices are directly transmitted to the cloud server without processing, delaying network connection and increasing costs. Edge computing has emerged to bridge these gaps by shifting partial data storage and computation capability from the cloud server to edge devices. However, selecting which tasks can be applied in edge computing depends on user-specific demands, suggesting the necessity to design a suitable Smart Agriculture Information System (SAIS) architecture for single-crop requirements. This study aims to design and implement a cost-saving multilayered SAIS architecture customized for smart greenhouse mushroom cultivation toward leveraging edge computing. A three-layer SAIS adopting the Device-Edge-Cloud protocol, which enables the integration of key environmental parameter data collected from the IoT sensor and RGB images collected from the camera, was tested in this research. Implementation of this designed SAIS architecture with typical examples of mushroom cultivation indicated that low-cost data pre-processing procedures including small-data storage, temporal resampling-based data reduction, and lightweight artificial intelligence (AI)-based data quality control (for anomalous environmental conditions detection) together with real-time AI model deployment (for mushroom detection) are compatible with edge computing. Integrating the Edge Layer as the center of the traditional protocol can significantly save network resources and operational costs by reducing unnecessary data sent from the device to the cloud, while keeping sufficient information.
Yield Gap Analysis of Super High-Yielding Rice (>15 t ha−1) in Two Ecological Regions
Authors: Wei Zhongwei , Zhang Yuzhu , Jin Wenyu
Published: March 2024

Super high-yielding rice (SHYR) (>15 t ha−1) plays a crucial role in global food production and security. We hypothesized that the external environment of different ecological regions could improve biomass accumulation in different periods and thus increase the rice yield. Two SHYR varieties, i.e., Xiangliangyou900 (XLY900) and Yliangyou900 (YLY900), were cultivated in the YONGSHENG and LONGHUI ecoregions, China. The results indicated that the average yield of the two SHYRs in the LONGHUI ecological region was 15.27–15.45 t ha−1 and 18.81–20.10 t ha−1 in YONGSHENG. The high grain yield in the YONGSHENG ecoregion was mainly due to the increased number of spikelets per panicle, crop growth rate, and total biomass during the transplanting–heading stage (TP-HS) and heading–maturity stage (HS-MS), and harvest index. The yield of SHYR was significantly correlated with external environment conditions, i.e., average minimum temperature, average daytime, and night-time temperature, and average daily temperature at the TP-HS, HS-MS, and transplanting–maturity (TP-MS) stages. The rice yield was significantly and positively correlated with the cumulative daily radiation. Therefore, it can be concluded that the final yield of super high-yield rice is closely related to the utilization of temperature and radiation resources during the growth process in the ecological environment.
Efficiency Factors in the Olive Oil Sector in Turkey
Authors: Noman Yousuf Abdulmunem , Fernández Uclés Domingo
Published: March 2024

Turkey ranks among the top five olive oil-producing countries in the world, and the olive crop plays a crucial role in its economy, economically, environmentally, and socially. One of the primary challenges facing the agricultural sector is its profitability. Therefore, the aim of this study is to analyse the olive sector in terms of economic efficiency, to identify productive and organizational variables directly associated with higher economic efficiency. Data were obtained from 193 organizations in the sector. A dual methodology is employed, comprising Data Envelopment Analysis (DEA) and, subsequently, Qualitative Comparative Analysis (QCA). The findings highlight the relevance of variables such as organization size, irrigation usage, focus on olive oil, or cultivation on sloping terrain as factors associated with a higher level of economic efficiency.
Nano-Food Farming: Toward Sustainable Applications of Proteins, Mushrooms, Nano-Nutrients, and Nanofibers
Authors: Prokisch József , Törős Greta , Nguyen Duyen H. H. , Neji Chaima , Ferroudj Aya , Sári Daniella , Muthu Arjun , Brevik Eric C. , El-Ramady Hassan
Published: March 2024

The relationship between agriculture and food is very close. It is impossible to produce adequate crops for global food security without proper farm management. Farming practices represent direct and indirect controlling factors in terms of global food security. Farming management practices influence agro-food production from seed germination through to the post-harvest treatments. Nano-farming utilizes nanotechnologies for agricultural food production. This review covers four key components of nano-farming: nano-mushroom production, protein-based nanoparticles, nano-nutrients, and nanofibers. This provides a comprehensive overview of the potential applications of nanotechnology in agriculture. The role of these components will be discussed in relation to the challenges faced and solutions required to achieve sustainable agricultural production. Edible mushrooms are important to food security because they are a nutritious food source and can produce nanoparticles that can be used in the production of other food sources. Protein-based nanoparticles have considerable potential in the delivery of bioactives as carriers and other applications. Nano-nutrients (mainly nano-selenium, nano-tellurium and carbon nanodots) have crucial impacts on the nutrient status of plant-based foods. Carbon nanodots and other carbon-based nanomaterials have the potential to influence agricultural crops positively. There are promising applications of nanofibers in food packaging, safety and processing. However, further research is needed to understand the impacts and potential risks of nanomaterials in the food production system.
Identification of Novel QTL for Seedling Root Architectural Traits in the D Genome of Natural and Resynthetic Allohexaploid Wheat
Authors: Wang Huifang , Yang Bangbang , Zhao Xinyu , Chen Hailong , Liu Fei , Ru Yating , Wei Xirui , Fu Xiaofeng , Guo Weiwei , Li Ximei , Golub Nataliia , Zhang Yumei
Published: March 2024

Root architectural traits at the seedling stage have been demonstrated to be crucial for the efficient uptake of nutrients and drought tolerance in wheat. To dissect the genetic basis of these traits from the D genome, 182 recombinant inbred lines (RILs) derived from the common wheat TAA10 crossed with resynthesized allohexaploid wheat XX329 possessed similar AABB genomes were used for QTL mapping of five root traits in hydroponic-cultured seedlings, including lateral root number (LRN), seminal root number (SRN), root hair length (RHL), root diameter (RD), and total root volume (TRV). A total of seven QTLs were identified for the five root traits, with six possible novel QTLs for LRN, RHL, RD and TRV, accounting for 4.98–12.17% of phenotypic variation. One QTL (QLrn.qau-5D.2), controlling lateral root number, was fine mapped an approximate 5.0-Mb interval harboring 80 annotated genes, including five auxin-related genes. We further validated that QLrn.qau-5D.2 in NILTAA10 significantly enhanced yield-related traits, such as plant height, spike length, spike compactness, tiller number per plant and grain yield per plant, as comparison with NILXX329. Collectively, these results provide vital insights for fine-mapping QTLs associated with LRN, SRN, RHL, RD and TRV and facilitate the root morphologic designs for enhancing yield performance.
An Evaluation of Potato Fertilization and the Potential of Farmers to Reduce the Amount of Fertilizer Used Based on Yield and Nutrient Requirements
Authors: Wang Yayi , Zhang Rong , Li Songling , Guo Xinnian , Li Quanhui , Hui Xiaoli , Wang Zhaohui , Wang Huixia
Published: March 2024

Unreasonable fertilization often fails to match crop yield and nutrient requirements, leading to low crop yield, the waste of mineral resources, and increased costs for farmers. A survey of the potato yield and fertilization of farmers was conducted in Haidong City of Qinghai Province for three consecutive years (2017–2019) torecommend reasonable fertilizer application. The results showed that the required amount of NPK fertilizer per ton of potato was 4.85 kg N, 1.26 kg P2O5, and 6.98 kg K2O, respectively. The potato yields ranged from 7500 to 66,429 kg ha−1, with an average of 26,069 kg ha−1. The average N, P, and Kfertilizers (in the form of N, P2O5, and K2O, respectively) applied by the farmers were 213 kg N ha−1, 202 kg P2O5ha−1, and 43 kg K2O ha−1. More than 60% of the farmers appliedtoo much Nand Pfertilizers and too little Kfertilizer. Therefore, the farmers with lowyieldsshould reduce the application of Nand P fertilizers by a range of 41–220 kg ha−1 and 24–265 kg ha−1. Allthe farmers should increase Kfertilizer use by 7–273 kg ha−1. Reasonable fertilization could effectively conserve resources, improve economic efficiency, and reduce environmental impact.
Development of a 3D Microfluidic Analytical Device for the Detection of Pathogenic Bacteria in Commercial Food Samples with Loop-Mediated Isothermal Amplification
Authors: Papatheodorou Spyridon-Andreas , Houhoula Dimitra , Magoulas Sotirios , Tsantes Andreas G. , Tsakali Efstathia , Akkermans Simen , Van Impe Jan , Halvatsiotis Panagiotis , Tsantes Argyrios E.
Published: March 2024

Traditional methods of detecting foodborne pathogens take several days to produce the required results. Furthermore, various molecular techniques (e.g., PCR) that also produce reliable results in the detection of pathogenic bacteria have been introduced, but the cost–time ratio required does not allow them to be considered a substantial solution to this specific problem. Three-dimensional (3D) printing technology provides the ability to design and manufacture microfluidic analytical devices using conventional 3D printers, which, in combination with colorimetric loop-mediated isothermal amplification (LAMP), may further simplify the process. The overall reduction in time and cost may provide the opportunity to upscale this diagnostic modality. Moreover, unlike most microfluidic analytical devices, this technique is simpler and more user-friendly, as it does not require any expertise or additional equipment apart from a conventional oven. A 3D-printed microfluidic analytical device in combination with LAMP was developed and tested for the simultaneous detection of foodborne pathogens in food samples. A total of 150 commercial food specimens (50 milk, 50 chicken, 50 lettuce samples) were analyzed for possible contamination with Salmonella typhimurium, Listeria monocytogenes and Escherichia coli. The 3D-printed microfluidic device was 100% precise for both negative (80 samples) and positive samples (7 samples were positive for S. typhimurium, 28 for L. monocytogenes, and 35 for E. coli) for all pathogens. Overall, the amount of data analyzed led to a high level of confidence in the precision of this device. As such, this new 3D device in combination with LAMP provides a precise detection method for food pathogens with a low detection limit.
Analytical Compatibility between Various Techniques for Determining Dissolved Manganese in Produced Water
Authors: Manea Gabriela Knippelberg Bifano , Freire Vanusa Bueno , Junior Rainerio Escalfoni , de Oliveira Elcio Cruz
Published: March 2024

Petroleum-produced water is the primary effluent generated during the oil extraction process, presenting high flow rates, and containing a series of chemical components that are harmful to the environment, such as dissolved manganese. The proof of metrological compatibility between laboratories and different test methods or techniques is an analytical challenge when complying with environmental laws and regulations, which is the aim of this study. The first step of this study was to compare the results of manganese content in real test samples of produced water between some laboratories that used different test methods, that is, flame atomic absorption spectrophotometry, inductively coupled plasma optical emission spectroscopy, and inductively coupled plasma mass spectrometry. Based on one-way analysis of variance, systematic errors were found. The following step was to visit a specific lab, where one could confirm the relevance of the wavelength parameters, operating conditions for the view configuration, and effects of the saline matrices. Finally, a compatibility assessment between the reference laboratory and a new laboratory, which implemented and validated its analytical methodology based on these analytical adjustments, and the experiences and lessons learned in the previous step, was undertaken by a paired t-test.
Treatment of Three Ferrets Diagnosed with Ferret Systemic Coronaviral Disease Using the Nucleoside Analogue GS-441524
Authors: Puffal Julia , Neece Amanda J. , Scaletti Federica
Published: March 2024

Ferret Systemic Coronaviral Disease (FSCD) is a systemic disease caused by ferret systemic coronavirus, which is considered lethal in most of the ferrets that are affected by it. To our knowledge, no treatment has been shown to be effective against FSCD in vivo, and most of the ferrets are euthanized or die after the development of clinical disease. GS-441524 has been shown to be effective in successfully treating cats with Feline Infectious Peritonitis (FIP), a disease that shares similarities with FSCD. However, to our knowledge, treatment with GS-441524 has not been reported for the treatment of FSCD in ferrets. Here, we describe three cases of ferrets diagnosed with FSCD successfully cured utilizing oral GS-441524. FSCD may be effectively treated following similar protocols utilized for feline infectious peritonitis in cats.
Effects of Methionine Supplementation Levels in Normal or Reduced Protein Diets on the Body Composition and Femur Bone Characteristics of Broilers Challenged with Coccidia
Authors: Liu Guanchen , Choppa Venkata Sesha Reddy , Sharma Milan Kumar , Ko Hanseo , Choi Janghan , Kim Woo Kyun
Published: March 2024

This study investigated the effects of dietary methionine (Met) levels on the bone quality of broilers challenged with coccidia. A total of 600 fourteen-day-old male Cobb500 broilers were gavaged with mixed Eimeria spp. and randomly allocated into 10 treatment groups by a 2 × 5 factorial arrangement. Birds received normal protein diets (NCP) or reduced-protein diets (LCP), containing 2.8, 4.4, 6.0, 7.6, and 9.2 g/kg of Met. Data were analyzed via two-way ANOVA and orthogonal polynomial contrast. At 9 days post-inoculation (DPI), whole body bone mineral density (BMD) and bone mineral content (BMC) linearly decreased as Met levels increased (p < 0.05). For the femoral metaphysis bone quality at 9 DPI, BMD linearly decreased, and porosity linearly increased as Met levels increased (p < 0.05) in the cortical bone. The increased Met levels linearly improved trabecular bone quality in LCP groups (p < 0.05) while not in NCP groups. For the femoral diaphysis cortical bone at 6 DPI, LCP groups had higher BMD and BMC than NCP groups (p < 0.05). Bone volume linearly increased as Met levels increased in LCP groups (p < 0.05) while not in NCP groups. In summary, the results suggested that increased Met levels decreased the cortical bone quality. However, in the context of reduced-protein diets, the increased Met levels improved trabecular bone quality.
Objective Assessment of Equine Locomotor Symmetry Using an Inertial Sensor System and Artificial Intelligence: A Comparative Study
Authors: Calle-González Natalie , Lo Feudo Chiara Maria , Ferrucci Francesco , Requena Francisco , Stucchi Luca , Muñoz Ana
Published: March 2024

In horses, quantitative assessment of gait parameters, as with the use of inertial measurement units (IMUs) systems, might help in the decision-making process. However, it requires financial investment, is time-consuming, and lacks accuracy if displaced. An innovative artificial intelligence marker-less motion tracking system (AI-MTS) may overcome these limitations in the field. Our aim was to compare the level of agreement and accuracy between both systems and visual clinical assessment. Twenty horses underwent locomotion analysis by visual assessment, IMUs, and AI-MTS systems, under the following conditions: straight hard (SH), straight soft (SS), left and right circle hard (LCH, RCH), and soft (LCS, RCS). A greater number of horses were considered sound by clinical examination, compared to those identified as symmetric by the two gait analysis systems. More limbs were considered asymmetric by the AI-MTS compared to IMUs, suggesting its greater sensitivity. The greatest agreement between the two systems was found for the difference between two minima in vertical head position in SH, while the lowest for the difference between two minima in vertical pelvis position in SS, reflecting the difficulties in assessing asymmetry of the hindlimbs. It is unknown what degree of asymmetry is clinically relevant, suggesting that more consistent use in training horses may help determine the thresholds for asymmetry. Some degree of asymmetry may be clinically relevant, suggesting its regular use in training horses.
YAP1/TAZ Mediates Rumen Epithelial Cell Proliferation but Not Short-Chain Fatty Acid Metabolism In Vitro
Authors: Yang Bin , Xu Zebang , Chen Hongwei , Ma Tingting , Zhao Yiming , Pang Mengxin , Wang Jiakun
Published: March 2024

Promoting rumen development is closely related to the health and efficient growth of ruminants. The transcriptional co-activators Yes1-associated protein (YAP1) and WW domain-containing transcription regulator protein 1 (TAZ) are key regulators of the mammalian epithelium. In the present study, we assessed the impact of YAP1/TAZ on rumen epithelial (RE) cell proliferation using their activator GA-017 (GA) and inhibitor verteporfin (VP). We also investigated whether YAP1/TAZ-dependent alteration was involved in the RE developmental process induced by sodium butyrate (SB). The results indicated that GA promoted RE cell proliferation, while VP disrupted RE cell proliferation. The Hippo, Wnt, and calcium signaling pathways were altered following the regulation of YAP1/TAZ. Upon YAP1/TAZ activation, the expression of CCN1/2 increased. However, when YAP1/TAZ was inhibited, the expression of BIRC3 decreased. In the SB-treated cells, YAP1/TAZ-induced changes were not observed. SB increased the expressions of differentiated cell marker genes and genes involved in short-chain fatty acid (SCFA) metabolism, while YAP1/TAZ did not. Thus, YAP1/TAZ could be potential targets for regulating RE cell proliferation but not for SCFA metabolism. SB could not affect YAP1/TAZ. These findings broaden our understanding of the role of YAP1/TAZ and their regulators in RE development.