bluuebunny/crossref_metadata_2025 huggingface dataset:
Ultra-high resolution, 3-dimensional magnetic resonance imaging of the atherosclerotic vessel wall at clinical 7T
Authors: Willemink Martin J. , Coolen Bram F. , Dyvorne Hadrien , Robson Philip M. , Bander Ilda , Ishino Seigo , Pruzan Alison , Sridhar Arthi , Zhang Bei , Balchandani Priti , Mani Venkatesh , Strijkers Gustav J. , Nederveen Aart J. , Leiner Tim , Fayad Zahi A. , Mulder Willem J. M. , Calcagno Claudia
Published: December 2020

Accurate quantification and characterization of atherosclerotic plaques with MRI requires high spatial resolution acquisitions with excellent image quality. The intrinsically better signal-to-noise ratio (SNR) at high-field clinical 7T compared to the widely employed lower field strengths of 1.5 and 3T may yield significant improvements to vascular MRI. However, 7T atherosclerosis imaging also presents specific challenges, related to local transmit coils and B1 field inhomogeneities, which may overshadow these theoretical gains. We present the development and evaluation of 3D, black-blood, ultra-high resolution vascular MRI on clinical high-field 7T in comparison lower-field 3T. These protocols were applied for in vivo imaging of atherosclerotic rabbits, which are often used for development, testing, and validation of translatable cardiovascular MR protocols. Eight atherosclerotic New Zealand White rabbits were imaged on clinical 7T and 3T MRI scanners using 3D, isotropic, high (0.63 mm3) and ultra-high (0.43 mm3) spatial resolution, black-blood MR sequences with extensive spatial coverage. Following imaging, rabbits were sacrificed for validation using fluorescence imaging and histology. Image quality parameters such as SNR and contrast-to-noise ratio (CNR), as well as morphological and functional plaque measurements (plaque area and permeability) were evaluated at both field strengths. Using the same or comparable imaging parameters, SNR and CNR were in general higher at 7T compared to 3T, with a median (interquartiles) SNR gain of +40.3 (35.3–80.1)%, and a median CNR gain of +68.1 (38.5–95.2)%. Morphological and functional parameters, such as vessel wall area and permeability, were reliably acquired at 7T and correlated significantly with corresponding, widely validated 3T vessel wall MRI measurements. In conclusion, we successfully developed 3D, black-blood, ultra-high spatial resolution vessel wall MRI protocols on a 7T clinical scanner. 7T imaging was in general superior to 3T with respect to image quality, and comparable in terms of plaque area and permeability measurements.
Chronic kidney disease as a risk factor for peripheral nerve impairment in older adults: A longitudinal analysis of Health, Aging and Body Composition (Health ABC) study
Authors: Doshi Simit , Moorthi Ranjani N. , Fried Linda F. , Sarnak Mark J. , Satterfield Suzanne , Shlipak Michael , Lange-Maia Brittney S. , Newman Anne B. , Strotmeyer Elsa S.
Published: December 2020

Introduction

Sensory and motor nerve deficits are prevalent in older adults and are associated with loss of functional independence. We hypothesize that chronic kidney disease predisposes to worsening sensorimotor nerve function over time.

Materials and methods

Participants were from the Health, Aging and Body Composition Study (N = 1121) with longitudinal data between 2000–01 (initial visit) and 2007–08 (follow-up visit). Only participants with non-impaired nerve function at the initial visit were included. The predictor was presence of CKD (estimated GFR ≤ 60 ml/min/1.73m2) from the 1999–2000 visit. Peripheral nerve function outcomes at 7-year follow-up were 1) Motor: “new” impairments in motor parameters (nerve conduction velocity NCV < 40 m/s or peroneal compound motor action potential < 1 mv) at follow-up, and 2) Sensory: “new” impairment defined as insensitivity to standard 10-g monofilament or light 1.4-g monofilament at the great toe and “worsening” as a change from light to standard touch insensitivity over time. The association between CKD and “new” or “worsening” peripheral nerve impairment was studied using logistic regression.

Results

The study population was 45.9% male, 34.3% Black and median age 75 y. CKD participants (15.6%) were older, more hypertensive, higher in BMI and had 2.37 (95% CI 1.30–4.34) fold higher adjusted odds of developing new motor nerve impairments in NCV. CKD was associated with a 2.02 (95% CI 1.01–4.03) fold higher odds of worsening monofilament insensitivity. CKD was not associated with development of new monofilament insensitivity.

Conclusions

Pre-existing CKD leads to new and worsening sensorimotor nerve impairments over a 7-year time period in community-dwelling older adults.
Demonstration of a fast and easy sample-to-answer protocol for tuberculosis screening in point-of-care settings: A proof of concept study
Authors: Ali Nasir , Bello Graziele Lima , Rossetti Maria Lúcia Rosa , Krieger Marco Aurelio , Costa Alexandre Dias Tavares
Published: December 2020

We sought to develop a smooth and low cost sample preparation and DNA extraction protocol, streamlined with a ready-to-use qPCR in a portable instrument to overcome some of the existing hurdles. Several solutions were evaluated as to their ability to liquefy a mucin-based matrix. Each liquefied matrix, supplemented with either Mycobacterium tuberculosis (MTB) H37Rv strain DNA or intact cells, was aliquoted onto a filter paper embedded with solubilizing agents, and was subsequently dried up. Most of the nucleic acids, including genomic DNA from the bacilli and the host, binds to the filter paper. Next, several protocols were evaluated to elute the DNA from the paper, using qPCR to detect the insertion sequence IS6110, a M. tuberculosis complex genomic marker. The limit of detection (LOD) of the best protocol was then evaluated using parallel seeding and colony counting. The protocol was also evaluated using seventeen sputum samples, previously characterized by the GeneXpert or culture. Two instruments (the ABI7500 Standard and the Q3-Plus system) and two reagents storage formats (frozen or ready-to-use) were evaluated. Solutions containing guanidine isothiocyanate exerted the best liquefying effect on the mucin-based matrix extracted from one 6-mm punches, followed by a brief incubation at 95°C. The resulting DNA contained impurities, but a simple 1:10 dilution elicited the detection of MTB and human genomic targets. The described protocol presented an apparent LOD of 02 CFU/mL of MTB. Challenging the protocol with previously characterized samples showed substantial agreement with GeneXpert MTB/RIF results (sensitivity of 90%, agreement of 88.9%, kappa coefficient of 0.77), and moderate agreement with culture results (sensitivity of 100%, agreement of 78.9%, kappa coefficient of 0.58). This work presents a sensitive proof–of-concept protocol for sputum liquefaction and decontamination followed by a simple DNA extraction procedure, in which the extraction steps are streamlined with a ready-to-use qPCR in a portable instrument that can be employed in low infrastructure settings.
Destabilizing effects on a classic tri-trophic oyster-reef cascade
Authors: Schweiss Virginia R. , Rakocinski Chet F.
Published: December 2020

How interactions among multiple predators affect the stability of trophic cascades is a topic of special ecological interest. To examine factors affecting the stability of the classic tri-trophic oyster reef cascade within a different context, configurations of three predators, including the Gulf toadfish, Gulf stone crab, and oystershell mud crab, were manipulated together with either oyster shell or limestone gravel substrate within a multiple predator effects (MPE) experiment. Additionally, a complimentary set of trait-mediated-indirect interaction (TMII) experiments examined the inhibition of oyster consumption relative to mud-crab size and top predator identity in the absence of other cues and factors. The classic tri-trophic cascade formed by the toadfish-mud crab-oyster configuration was potentially weakened by several interactions within the MPE experiment. Consumption of oysters and mud crabs by the intraguild stone crab was undeterred by the presence of toadfish. Although mud crab feeding was inhibited in the presence of both toadfish and stone crabs, estimated non-consumptive effects (NCEs) were weaker for stone crabs in the MPE experiment. Consequently, the total effect was destabilizing when all three predator species were together. Inhibition of mud crab feeding was inversely related to direct predation on mud crabs within the MPE experiment. Complimentary TMII experiments revealed greater inhibition of mud crab feeding in response to stone crabs under sparse conditions. TMII experiments also implied that inhibition of mud crab feeding could have largely accounted for NCEs relative to oysters within the MPE experiment, as opposed to interference by other mud crabs or top predators. An inverse relationship between mud crab size and NCE strength in the TMII experiment disclosed another potentially destabilizing influence on the tri-trophic-cascade. Finally, although habitat complexity generally dampened the consumption of oysters across MPE treatments, complex habitat promoted mud crab feeding in the presence of toadfish alone. This study underscores how ecological interactions can mediate trophic cascades and provides some additional insights into the trophic dynamics of oyster reefs for further testing under natural conditions.
The benefit of adjuvant radiotherapy on overall survival in resected stage I to II pancreatic cancer: A propensity-adjusted analysis
Authors: Lin Zhuang-Bin , Song Jian-Yuan , Li An-Chuan , Chen Cheng , Huang Xiao-Xue , Xu Ben-Hua
Published: December 2020

Background

The survival time of patients with early pancreatic cancer (PC) is still disappointing, even after surgical resection. PC has an extremely poor prognosis. Herein, we aimed to investigate the survival effect of postoperative radiotherapy (PORT) on resected stage I to II PC.

Material and methods

A large eligible sample of patients was identified from 2010 to 2015 from the Surveillance, Epidemiology, and End Results (SEER) registry. Survival analysis was conducted to evaluate the efficiency of PORT. Propensity score matching (PSM) analysis was used to reduce selection bias and to make the groups comparable.

Results

A total of 3219 patients with resected stage I to II PC was included after rigid screening. The median overall survival (OS) was 26 months with PORT (n = 1055) versus 21 months with non-PORT (n = 2164) before matching (p<0.001). By multivariable analysis, PORT remained a favorable prognostic predictor for OS. In PSM analysis, receiving PORT was associated with improved OS (median, 26 months vs. 23 months; at 2 years, 51.7% vs. 46.7%; at 5 years, 23.3% vs. 17.4% (P = 0.006). After further meticulous exploration, only the stage IIB subgroup benefited from PORT (p<0.001). This result was due to the positive lymph node state (N+), whose mortality risk was cut by 23.4% (p<0.001) by PORT.

Conclusion

Addition of PORT to the treatment of patients with resected stage I to II PC conveys a survival benefit, particularly among those with N-positive or stage IIB disease.
ANOVA-HD: Analysis of variance when both input and output layers are high-dimensional
Authors: de los Campos Gustavo , Pook Torsten , Gonzalez-Reymundez Agustin , Simianer Henner , Mias George , Vazquez Ana I.
Published: December 2020

Modern genomic data sets often involve multiple data-layers (e.g., DNA-sequence, gene expression), each of which itself can be high-dimensional. The biological processes underlying these data-layers can lead to intricate multivariate association patterns. We propose and evaluate two methods to determine the proportion of variance of an output data set that can be explained by an input data set when both data panels are high dimensional. Our approach uses random-effects models to estimate the proportion of variance of vectors in the linear span of the output set that can be explained by regression on the input set. We consider a method based on an orthogonal basis (Eigen-ANOVA) and one that uses random vectors (Monte Carlo ANOVA, MC-ANOVA) in the linear span of the output set. Using simulations, we show that the MC-ANOVA method gave nearly unbiased estimates. Estimates produced by Eigen-ANOVA were also nearly unbiased, except when the shared variance was very high (e.g., >0.9). We demonstrate the potential insight that can be obtained from the use of MC-ANOVA and Eigen-ANOVA by applying these two methods to the study of multi-locus linkage disequilibrium in chicken (Gallus gallus) genomes and to the assessment of inter-dependencies between gene expression, methylation, and copy-number-variants in data from breast cancer tumors from humans (Homo sapiens). Our analyses reveal that in chicken breeding populations ~50,000 evenly-spaced SNPs are enough to fully capture the span of whole-genome-sequencing genomes. In the study of multi-omic breast cancer data, we found that the span of copy-number-variants can be fully explained using either methylation or gene expression data and that roughly 74% of the variance in gene expression can be predicted from methylation data.
Highways to happiness for autistic adults? Perceived causal relations among clinicians
Authors: Deserno Marie K. , Borsboom Denny , Begeer Sander , van Bork Riet , Hinne Max , Geurts Hilde M.
Published: December 2020

The network approach to psychological phenomena advances our understanding of the interrelations between autism and well-being. We use the Perceived Causal Relations methodology in order to (i) identify perceived causal pathways in the well-being system, (ii) validate networks based on self-report data, and (iii) quantify and integrate clinical expertise in autism research. Trained clinicians served as raters (N = 29) completing 374 cause-effects ratings of 34 variables on well-being and symptomatology. A subgroup (N = 16) of raters chose intervention targets in the resulting network which we found to match the respective centrality of nodes. Clinicians’ perception of causal relations was similar to the interrelatedness found in self-reported client data (N = 323). We present a useful tool for translating clinical expertise into quantitative information enabling future research to integrate this in scientific studies.
High school students’ use of JUUL pod flavors before and after JUUL implemented voluntary sales restrictions on certain flavors in 2018
Authors: Morean Meghan E. , Bold Krysten W. , Kong Grace , Camenga Deepa R. , Jackson Asti , Simon Patricia , Davis Danielle R. , Krishnan-Sarin Suchitra
Published: December 2020

Objectives

In November 2018, JUUL, Inc. restricted sales of mango, fruit medley, crème brûlée, and cucumber pods to its website. Physical/online sales of tobacco, menthol, and mint pods continued. We examined rates of adolescent JUUL device and flavored pod use before and after restrictions were implemented to examine their potential impact.

Methods

Students from 4 Connecticut high schools completed cross-sectional surveys in 2018 (N = 3170) and 2019 (N = 3074).

Results

Compared to 2018, current (past-month) JUUL use decreased in 2019 (30.2–25.6%). Among current JUUL users, restricted flavor use (mango [62.8–36.9%]; cucumber [27.7–11.9%]; fruit [23.5–11.4%]; crème brûlée [12.3–5.0%]) and the total number of flavors used (2.09[SD = 1.71]-1.51[SD = 1.33]) decreased (p-values < .001), while mint pod use increased (62.0–68.6%, p < .01). Tobacco and menthol pod use remained stable.

Conclusions

Following voluntary sales restrictions implemented by JUUL, Inc., restricted flavor use and the total number of flavors used decreased among JUUL users while mint use increased. Results suggest flavor restrictions may impact adolescents’ e-cigarette use. While 2020 Food and Drug Administration restrictions on mint pods may further limit youth JUUL use, research is needed to determine where youth acquire restricted flavors and if restrictions prompt increased popularity of unrestricted devices/e-liquids.
1H NMR metabolic phenotyping of Dipterocarpus alatus as a novel tool for age and growth determination
Authors: Phetcharaburanin Jutarop , Deewai Suthicha , Kulthawatsiri Thanaporn , Moolpia Komkid , Suksawat Manida , Promraksa Bundit , Klanrit Poramate , Namwat Nisana , Loilome Watcharin , Poopasit Kitisak , Katekaew Somporn , Phetcharaburanin Penprapa
Published: December 2020

Dipterocarpus alatus belongs to Family Dipterocarpaceae that can be commonly found in Southeast Asian countries. It is a perennial plant with oval-shaped leaves and oleoresin-rich wood. It has been considered as a multipurpose plant since all parts can be practically utilized. One of the major problems for utilizing Dipterocarpus alatus is the difficulty knowing the exact age as this kind of plant is ready for multipurpose use after 20 years of age. At present, the most commonly used method for determining age of Dipterocarpus alatus is the annual ring estimation. However, this conventional method is unable to provide the high precision and accuracy of age determination due to its limitation including blurry annual rings caused by enriched oleoresin in the wood. The current study aimed to investigate the differences of 1H -NMR spectroscopy-based metabolic profiles from bark and leaf of Dipterocarpus alatus at different ages including 2, 7, 15 and 25 years. Our findings demonstrated that there is a total of 56 metabolites shared between bark and leaf. It is noticeable that bark at different ages exhibited the strongest variation and sugar or sugar derivatives that were found in higher concentrations in bark compared with those in leaf. We found that decreasing levels of certain metabolites including tagatose, 1’kestose and 2’-fucosyllactose exhibited the promising patterns. In conclusion, panel metabolites involved in the sucrose biosynthesis can precisely determine the age and growth of Dipterocarpus alatus.
Stomathognatic system function in indigenous people from Brazilian Xingu villages: An electromyographic analysis
Authors: Moreto Santos Carla , Palinkas Marcelo , Mestriner-Júnior Wilson , Hallak Regalo Isabela , Batista de Vasconcelos Paulo , José Dias Fernando , Eduardo Cecilio Hallak Jaime , Siéssere Selma , Cecilio Hallak Regalo Simone
Published: December 2020

The maintenance of postural balance can be influenced by the lifestyle of a population. This study aimed to determine the electromyographic activity of the masseter and temporalis muscles during mandibular tasks and habitual and non-habitual chewing in indigenous individuals to reveal the differences among white Brazilian individuals. Sixty Brazilians (18 and 28 years) were divided into two groups: 30 Xingu indigenous individuals and 30 white Brazilian individuals, with 20 men and 10 women in each group. The individuals were assessed using the normalized electromyographic activity of mandibular tasks (rest, protrusion, right and left laterality) and electromyographic activity of masticatory cycles in habitual (peanuts and raisins) and non-habitual (Parafilm M) chewing. Data were analyzed using Student’s t-test (p < .05). Comparisons between the groups demonstrated significant differences. Indigenous individuals group presented a decrease in the normalized electromyographic activity of the masticatory muscles during mandibular rest [right masseter (p = .002) and left masseter (p = .004) muscles]. There was increase in the normalized electromyographic activity during protrusion [left temporal (p = .03) muscle]. There was increase in the electromyographic activity during chewing: peanuts [right masseter (p = .001), left masseter (p = .001) and right temporal (p = .01) muscles], raisins [right masseter (p = .001), left masseter (p = .002), right temporal (p = .008), left temporal (p = .01) muscles] and Parafilm M [left masseter muscle (p = .05)]. From the findings of this study, we concluded that in the comparison between indigenous and white individuals, positive changes were observed in the electromyographic pattern of the masticatory muscles in the mandibular postural conditions, with greater masticatory efficiency in the indigenous group.
SVA insertion in X-linked Dystonia Parkinsonism alters histone H3 acetylation associated with TAF1 gene
Authors: Petrozziello Tiziana , Dios Amanda M. , Mueller Kaly A. , Vaine Christine A. , Hendriks William T. , Glajch Kelly E. , Mills Alexandra N. , Mangkalaphiban Kotchaphorn , Penney Ellen B. , Ito Naoto , Fernandez-Cerado Cara , Legarda Gierold Paul A. , Velasco-Andrada M. Salvie , Acuña Patrick J. , Ang Mark A. , Muñoz Edwin L. , Diesta Cid Czarina E. , Macalintal-Canlas Regina , Acuña Geraldine , Sharma Nutan , Ozelius Laurie J. , Bragg D. Cristopher , Sadri-Vakili Ghazaleh
Published: December 2020

X-linked Dystonia-Parkinsonism (XDP) is a neurodegenerative disease linked to an insertion of a SINE-VNTR-Alu (SVA)-type retrotransposon within an intron of TAF1. This SVA insertion induces aberrant TAF1 splicing and partial intron retention, thereby decreasing levels of the full-length transcript. Here we sought to determine if these altered transcriptional dynamics caused by the SVA are also accompanied by local changes in histone acetylation, given that these modifications influence gene expression. Because TAF1 protein may itself exhibit histone acetyltransferase activity, we also examined whether decreased TAF1 expression in XDP cell lines and post-mortem brain affects global levels of acetylated histone H3 (AcH3). The results demonstrate that total AcH3 are not altered in XDP post-mortem prefrontal cortex or cell lines. We also did not detect local differences in AcH3 associated with TAF1 exons or intronic sites flanking the SVA insertion. There was, however, a decrease in AcH3 association with the exon immediately proximal to the intronic SVA, and this decrease was normalized by CRISPR/Cas-excision of the SVA. Collectively, these data suggest that the SVA insertion alters histone status in this region, which may contribute to the dysregulation of TAF1 expression.
Clinical characteristics and outcomes of critically ill patients with COVID-19 admitted to an intensive care unit in London: A prospective observational cohort study
Authors: Thomson Ross J. , Hunter Jennifer , Dutton Jonathan , Schneider James , Khosravi Maryam , Casement Alisha , Dhadwal Kulwant , Martin Daniel
Published: December 2020

Background

Cohorts of severely ill patients with COVID-19 have been described in several countries around the globe, but to date there have been few published reports from the United Kingdom (UK). Understanding the characteristics of the affected population admitted to intensive care units (ICUs) in the UK is crucial to inform clinical decision making, research and planning for future waves of infection.

Methods

We conducted a prospective observational cohort study of all patients with COVID-19 admitted to a large UK ICU from March to May 2020 with follow-up to June 2020. Data were collected from health records using a standardised template. We used multivariable logistic regression to analyse the factors associated with ICU survival.

Results

Of the 156 patients included, 112 (72%) were male, 89 (57%) were overweight or obese, 68 (44%) were from ethnic minorities, and 89 (57%) were aged over 60 years of age. 136 (87%) received mechanical ventilation, 77 (57% of those intubated) were placed in the prone position and 95 (70% of those intubated) received neuromuscular blockade. 154 (99%) patients required cardiovascular support and 44 (28%) required renal replacement therapy. Of the 130 patients with completed ICU episodes, 38 (29%) died and 92 (71%) were discharged alive from ICU. In multivariable models, age (OR 1.13 [95% CI 1.07–1.21]), obesity (OR 3.06 [95% CI 1.16–8.74]), lowest P/F ratio on the first day of admission (OR 0.82 [95% CI 0.67–0.98]) and PaCO2 (OR 1.52 [95% CI 1.01–2.39]) were independently associated with ICU death.

Conclusions

Age, obesity and severity of respiratory failure were key determinants of survival in this cohort. Multiorgan failure was prevalent. These findings are important for guiding future research and should be taken into consideration during future healthcare planning in the UK.
Cognitive job crafting as mediator between behavioral job crafting and quality of care in residential homes for the elderly
Authors: Romeo Marina , Yepes-Baldó Montserrat , Westerberg Kristina , Nordin Maria
Published: December 2020

Extending previous studies on job crafting, the aim of the present study is to analyze the effect of job crafting on quality of care in residential homes for elderly people in two European countries (Spain and Sweden). We hypothesize that cognitive crafting could be a consequence of behavioral crafting and that it will mediate the relationship between behavioral crafting and the perception of quality of care. A correlational design was used, with two-waves approximately 12 months apart (n = 226). Our results indicate that behavioral job crafting at T1 had an effect on cognitive job crafting at T2, relational job crafting at T1 increases quality of care at T2, and the mediation effect of cognitive job crafting. These results indicate that we must differentiate between the two forms of crafting (behavioral and cognitive), not as indicators of the same latent construct, but as aggregates. Additionally, we point out two main implications for managerial practice. First, as relational job crafting has a direct effect on quality of care, it is important to assure an organizational culture oriented towards employees. Secondly, due to the mediation effect of cognitive job crafting, managers should facilitate meaningful work environments. To do so, jobs should be re-designed, increasing skills variety, identity and significance.
Mediating effects of general self-efficacy on the relationship between the source of meaning in life and prosocial behaviours in vocational college nursing students: A cross-sectional study
Authors: Cheng Linan , Ye Yansheng , Zhong Zhaoyang , Zhang Fengying , Hu Xiuying , Cui Renshan , Chen Qian
Published: December 2020

Objectives

To examine the mediating effect of general self-efficacy on the relationship between the source of meaning in life (SML) and prosocial behaviours in vocational college nursing students.

Methods

Between March and June 2019, a cross-sectional descriptive study was conducted, and 799 nursing students from three vocational colleges completed the Source of Meaning in Life Scale, General Self-Efficacy Scale and Prosocial Behaviours Scale. Data were analyzed using structural equation modelling and statistical analysis by SPSS (version 23.0, IBM).

Results

The average SML, general self-efficacy and prosocial behaviours scores of the 799 nursing students were 6.43±0.83, 2.48±0.59 and 3.69±0.62, respectively. Correlation analysis showed that SML, general self-efficacy and prosocial behaviours were positively correlated (P<0.01). General self-efficacy partially mediated the relationship between SML and prosocial behaviours (P<0.01); this mediating effect contributed 22.97% of the total effect and explained 17.6% of the variance in the dependent variable.

Conclusions

Educators should focus on cultivating nursing students’ cognition and experience of meaning in life and their efficacy in life, study and work, which can improve students’ “people-oriented” service and prosocial behaviour and the quality of nursing services.
Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina
Authors: Oyuela Aguilar Mónica , Gobbi Alex , Browne Patrick D. , Ellegaard-Jensen Lea , Hansen Lars Hestbjerg , Semorile Liliana , Pistorio Mariano
Published: December 2020

Soil microbiomes, as a primary reservoir for plant colonizing fungi and bacteria, play a major role in determining plant productivity and preventing invasion by pathogenic microorganisms. The use of 16S rRNA and ITS high-throughput amplicon sequencing for analysis of complex microbial communities have increased dramatically in recent years, establishing links between wine specificity and, environmental and viticultural factors, which are framed into the elusive terroir concept. Given the diverse and complex role these factors play on microbial soil structuring of agricultural crops, the main aim of this study is to evaluate how external factors, such as vintage, vineyard location, cultivar and soil characteristics, may affect the diversity of the microbial communities present. Additionally, we aim to compare the influence these factors have on the structuring of bacterial and fungal populations associated with Malbec grapevine rhizosphere with that of the more widespread Cabernet Sauvignon grapevine cultivar. Samples were taken from Malbec and Cabernet Sauvignon cultivars from two different vineyards in the San Juan Province of Argentina. Total DNA extracts from the rhizosphere soil samples were sequenced using Illumina’s Miseq technology, targeting the V3-V4 hypervariable 16S rRNA region in prokaryotes and the ITS1 region in yeasts. The major bacterial taxa identified were Proteobacteria, Bacteroidetes and Firmicutes, while the major fungal taxa were Ascomycetes, Basidiomycetes, Mortierellomycetes and a low percentage of Glomeromycetes. Significant differences in microbial community composition were found between vintages and vineyard locations, whose soils showed variances in pH, organic matter, and content of carbon, nitrogen, and absorbable phosphorus.
Managing threatened ungulates in logged-primary forest mosaics in Malaysia
Authors: D. Mark Rayan , Linkie Matthew
Published: December 2020

Across the tropics, large-bodied mammals have been affected by selective logging in ways that vary with levels of timber extraction, collateral damage, species-specific traits and secondary effects of hunting, as facilitated by improved access through logging roads. In Peninsular Malaysia, 3.0 million hectares or 61 percent of its Permanent Reserved Forests is officially assigned for commercial selective logging. Understanding how wildlife adapts and uses logged forest is critical for its management and, for threatened species, their conservation. In this study, we quantify the population status of four tropical ungulate species in a large selectively logged forest reserve and an adjacent primary forest protected area. We then conduct finer scale analyses to identify the species-specific factors that determine their occurrence. A combined indirect sign-camera trapping approach with a large sampling effort (2,665 km and 27,780 trap nights surveyed) covering a wide area (560 km2) generated species-specific detection probabilities and site occupancies. Populations of wild boar were widespread across both logged and primary forests, whereas sambar and muntjac occupancy was lower in logged forest (48.4% and 19.2% respectively), with gaur showing no significant difference. Subsequent modelling revealed the importance of conserving lower elevation habitat in both habitat types, particularly <1,000 m asl, for which occupancies of sambar, muntjac and gaur were typically higher. This finding is important because 75 percent (~13,400 km2) of Peninsular Malaysia’s Main Range Forest (Banjaran Titiwangsa) is under 1,000 m asl and therefore at risk of being converted to industrial timber plantations, which calls for renewed thinking around forest management planning.
A prospective study to explore the relationship between MTHFR C677T genotype, physiological folate levels, and postpartum psychopathology in at-risk women
Authors: Morris Emily , Hippman Catriona , Albert Arianne , Slomp Caitlin , Inglis Angela , Carrion Prescilla , Batallones Rolan , Andrighetti Heather , Ross Colin , Dyer Roger , Honer William , Austin Jehannine
Published: December 2020

Background

The etiology of postpartum psychopathologies are not well understood, but folate metabolism pathways are of potential interest. Demands for folate increase dramatically during pregnancy, low folate level has been associated with psychiatric disorders, and supplementation may improve symptomatology. The MTHFR C677T variant influences folate metabolism and has been implicated in depression during pregnancy.

Objective

To conduct a prospective longitudinal study to explore the relationship between MTHFR C677T genotype, folate levels, and postpartum psychopathology in at-risk women.

Hypothesis

In the first three months postpartum, folate will moderate a relationship between MTHFR genotype and depression, with TT homozygous women having more symptoms than CC homozygous women.

Methods

We recruited 365 pregnant women with a history of mood or psychotic disorder, and at 3 postpartum timepoints, administered the Edinburgh Postnatal Depression Scale (EPDS); Clinician-Administered Rating Scale for Mania (CARS-M) and the Positive and Negative Symptom Scale (PANSS) and drew blood for genotype/folate level analysis. We used robust linear regression to investigate interactions between genotype and folate level on the highest EPDS and CARS-M scores, and logistic regression to explore interactions with PANSS psychosis scores above/below cut-off.

Results

There was no significant interaction effect between MTHFR genotype and folate level on highest EPDS (p = 0.36), but there was a significant interaction between genotype, folate level and log(CARS-M) (p = 0.02); post-hoc analyses revealed differences in the effect of folate level between CC/CT, and TT genotypes, with folate level in CC and CT having an inverse relationship with symptoms of mania, while there was no relationship in participants with TT genotype. There was no significant interaction between MTHFR genotype and folate level on the likelihood of meeting positive symptom criteria for psychosis on the PANSS (p = 0.86).

Discussion

These data suggest that perhaps there is a relationship between MTHFR C677T, folate level and some symptoms of postpartum psychopathology.
Epidemiology and the diagnostic challenge of extra-pulmonary tuberculosis in a teaching hospital in Ethiopia
Authors: Arega Balew , Mersha Amdemeskel , Minda Abraham , Getachew Yitagesu , Sitotaw Alazar , Gebeyehu Tefera , Agunie Asnake
Published: December 2020

Background

Ethiopia reported a high rate of extra-pulmonary tuberculosis (EPTB) and the cases are increasing since the last three decades. However, diagnostic evidence to initiate TB treatment among EPTB cases is not well known. Therefore, we described the epidemiology and assessed how EPTB is diagnosed in a teaching hospital in Ethiopia.

Methods

We conducted a retrospective review among all adult EPTB cases diagnosed in Yekatit 12 Hospital Medical College from 2015 to 2019. Using a standardized data abstraction sheet, we collected data from patients’ medical records on sociodemographic, sites, and laboratory diagnosis of EPTB cases.

Results

Of the 965 total TB cases, 49.8%(481) had a recorded diagnosis of EPTB during the study period. The mean age of EPTB patients was 32.9 years (SD±13.9) and 50.7% were males. Tubercular lymphadenitis (40.3%), abdominal (23.4%), and pleural TB(13.5%) were the most common sites of EPTB involvement, followed in descending order by the genitourinary, skeletal, central nervous system, abscess, breast, and laryngeal TB. We found a histopathology finding consistent with EPTB in 59.1% of cases, Acid-fast bacilli positive in 1.5%, and the rest diagnosed on radiological grounds. In the majority of cases, more than one diagnostic method was used to diagnose EPTB cases.

Conclusions

Nearly half of TB patients had a recorded diagnosis of EPTB that comprise heterogeneous anatomical sites. All EPTB patients were started anti-TB therapy without definitive microbiology results. This indicates the diagnostic challenge of EPTB faced in our setting and proves to be significant for TB control in Ethiopia.
Correlation between hospitalized patients’ demographics, symptoms, comorbidities, and COVID-19 pandemic in Bahia, Brazil
Authors: Macedo Márcio C. F. , Pinheiro Isabelle M. , Carvalho Caio J. L. , Fraga Hilda C. J. R. , Araujo Isaac P. C. , Montes Simone S. , Araujo Otávio A. C. , Alves Lucas A. , Saba Hugo , Araújo Márcio L. V. , Queiroz Ivonete T. L. , Sampaio Romilson L. , Souza Márcia S. P. L. , da Silva Ana Claudia F. N. , Souza Antonio C. S.
Published: December 2020

In this paper, we provide a retrospective cohort study with patients that have been hospitalized for general or intensive care unit admission due to COVID-19, between March 3 and July 29, 2020, in the state of Bahia, Brazil. We aim to correlate those patients’ demographics, symptoms and comorbidities, with the risk of mortality from COVID-19, length of hospital stay, and time from diagnosis to definitive outcome. On the basis of a dataset provided by the Health Secretary of the State of Bahia, we selected 3,896 hospitalized patients from a total of 154,868 COVID-19 patients that included non-hospitalized patients and patients with invalid registration in the dataset. Then, we statistically analyzed whether there was a significant correlation between the patient record data and the COVID-19 pandemic, and our main findings reinforced by the use of a multivariable logistic regression were that older age (Odds Ratio [OR] = 1.03, 95% Confidence Interval [CI] = 1.03-1.04, p-value (p) <0.001), an initial symptom of shortness of breath (OR = 1.88, 95% CI = 1.60-2.20, p < 0.001), and the presence of comorbidities, mainly chronic kidney disease (OR = 2.41, 95% CI = 1.67-3.48, p < 0.001) are related to an increased risk of mortality from COVID-19. On the other hand, sore throat (OR = 0.74, 95% CI = 0.58-0.95, p = 0.02) and length of hospital stay (OR = 0.96, 95% CI = 0.58-0.95, p < 0.001) are more related to a reduced risk of mortality from COVID-19. Moreover, a multivariable linear regression conducted with statistically significant variables (p < 0.05) showed that age (OR = 0.97, 95% CI = 0.95-0.98, p < 0.001) and time from diagnosis to definitive outcome (OR = 1.67, 95% CI = 1.64-1.71, p < 0.001) are associated with the length of hospital stay.
Associations between legal performance-enhancing substance use and future cardiovascular disease risk factors in young adults: A prospective cohort study
Authors: Nagata Jason M. , Ganson Kyle T. , Cunningham Mitchell L. , Mitchison Deborah , Lavender Jason M. , Blashill Aaron J. , Gooding Holly C. , Murray Stuart B.
Published: December 2020

Background

Legal performance-enhancing substances (PES), such as creatine, are commonly used by adolescents and young adults. As PES are mostly unregulated by the US Food and Drug Administration, there has been limited empirical attention devoted to examining their long-term safety and health outcomes. Preliminary studies have demonstrated associations between PES use and severe medical events, including hospitalizations and death. PES could be linked to cardiovascular disease (CVD), the most common cause of mortality in the US, by altering the myocardium, vasculature, or metabolism. The objective of this study was to examine prospective associations between the use of legal PES in young adulthood and CVD risk factors at seven-year follow-up.

Materials and methods

Nationally representative longitudinal cohort data from the National Longitudinal Study of Adolescent to Adult Health, Waves III (2001–2002) and IV (2008), were analyzed. Regression models determined the prospective association between the use of legal PES (e.g. creatine monohydrate) and CVD risk factors (e.g. body mass index, diabetes, hypertension, hyperlipidemia), adjusting for relevant covariates.

Results

Among the diverse sample of 11,996 male and female participants, no significant differences by PES use in body mass index, diabetes, hypertension, or hyperlipidemia were noted at Wave III. In unadjusted comparisons, legal PES users (versus non-users) were more likely to be White, be male, be college educated, drink alcohol, and engage in weightlifting, exercise, individual sports, team sports, and other strength training. There were no significant prospective associations between legal PES use at Wave III and body mass index, hemoglobin A1c, systolic and diastolic blood pressure, and cholesterol (total, HDL, LDL, triglycerides) deciles at seven-year follow-up (Wave IV), adjusting for demographics, health behaviors, and Wave III CVD risk factors. Similarly, there were no significant prospective associations between legal PES use and diabetes, hypertension, or hyperlipidemia based on objective measures or self-reported medications and diagnoses, adjusting for demographics, health behaviors, and Wave III CVD risk.

Conclusions

We do not find evidence for a prospective association between legal PES use and CVD risk factors in young adults over seven years of follow-up, including BMI, diabetes, hypertension, or hyperlipidemia. It should be noted that legal PES use was operationalized dichotomously and as one broad category, which did not account for frequency, amount, or duration of use. Given the lack of regulation and clinical trials data, observational studies can provide much needed data to inform the safety and long-term health associations of legal PES use and, in turn, inform clinical guidance and policy.
Factors affecting mental health of health care workers during coronavirus disease outbreaks (SARS, MERS & COVID-19): A rapid systematic review
Authors: De Brier Niels , Stroobants Stijn , Vandekerckhove Philippe , De Buck Emmy
Published: December 2020

Introduction

The novel Coronavirus Disease (COVID-19) outbreak currently puts health care workers at high risk of both physical and mental health problems. This study aimed to identify the risk and protective factors for mental health outcomes in health care workers during coronavirus epidemics.

Methods

A rapid systematic review was performed in three databases (March 24, 2020) and a current COVID-19 resource (May 28, 2020). Following study selection, study characteristics and effect measures were tabulated, and data were synthesized by using vote counting. Meta-analysis was not possible because of high variation in risk factors, outcomes and effect measures. Risk of bias of each study was assessed and the certainty of evidence was appraised according to the GRADE methodology.

Results

Out of 2605 references, 33 observational studies were selected and the identified risk and protective factors were categorized in ten thematic categories. Most of these studies (n = 23) were performed during the SARS outbreak, seven during the current COVID-19 pandemic and three during the MERS outbreak. The level of disease exposure and health fear were significantly associated with worse mental health outcomes. There was evidence that clear communication and support from the organization, social support and personal sense of control are protective factors. The evidence was of very low certainty, because of risk of bias and imprecision.

Conclusion

Safeguarding mental health of health care workers during infectious disease outbreaks should not be treated as a separate mental health intervention strategy, but could benefit from a protective approach. This study suggests that embedding mental health support in a safe and efficient working environment which promotes collegial social support and personal sense of control could help to maximize resilience of health care workers. Low quality cross-sectional studies currently provide the best possible evidence, and further research is warranted to confirm causality.
Host RAB11FIP5 protein inhibits the release of Kaposi’s sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45
Authors: Wei Xiaoqin , Dong Jiazhen , Cheng Chin-Chen , Ji Mingjun , Yu Lei , Luo Shengqiu , Wu Shuwen , Bai Lei , Lan Ke
Published: December 2020

Open reading frame (ORF) 45 is an outer tegument protein of Kaposi’s sarcoma-associated herpesvirus (KSHV). Genetic analysis of an ORF45-null mutant revealed that ORF45 plays a key role in the events leading to the release of KSHV particles. ORF45 associates with lipid rafts (LRs), which is responsible for the colocalization of viral particles with the trans-Golgi network and facilitates their release. In this study, we identified a host protein, RAB11 family interacting protein 5 (RAB11FIP5), that interacts with ORF45 in vitro and in vivo. RAB11FIP5 encodes a RAB11 effector protein that regulates endosomal trafficking. Overexpression of RAB11FIP5 in KSHV-infected cells decreased the expression level of ORF45 and inhibited the release of KSHV particles, as reflected by the significant reduction in the number of extracellular virions. In contrast, silencing endogenous RAB11FIP5 increased ORF45 expression and promoted the release of KSHV particles. We further showed that RAB11FIP5 mediates lysosomal degradation of ORF45, which impairs its ability to target LRs in the Golgi apparatus and inhibits ORF45-mediated colocalization of viral particles with the trans-Golgi network. Collectively, our results suggest that RAB11FIP5 enhances lysosome-dependent degradation of ORF45, which inhibits the release of KSHV particles, and have potential implications for virology and antiviral design.
Characterisation of activated charcoal, sawdust charcoal and rice husk charcoal as adsorbents in water treatment
Authors: Adekunle Adebola A. , Familusi Ayokunle O. , Badejo Adedayo A. , Adeosun Olayemi J. , Arogundade Suhaib A.
Published: December 2020

This study is an investigation into the characterization of commercial activated charcoal, sawdust charcoal and rice husk charcoal as adsorbents for water treatment. The ground rice husk and waste sawdust collected, were sieved to obtain a nominal size of 1mm, washed and oven-dried for 12hours. The two materials were pyrolysized in a furnace for 30minutes, and the chars produced were later air-dried. The three charcoals (sawdust charcoal, rice husk charcoal, and the activated charcoal purchased from the market) were all subjected to X-ray Fluorescence (XRF) analysis, Scanning Electron Microscope (SEM) analysis and Energy Dispersive X-ray (EDX) analysis in order to characterize the filter materials. The SEM analysis showed that the three materials developed more pores, which is a property of an adsorbent. Likewise, the XRF and EDX analyses confirm that all the three adsorbents possess larger proportion of Silica, Carbon and Oxygen.
Az európai mennyiségi lazítás jellemzői és perspektívái
Authors: Pál Tamás
Published: December 2020

Az Európai Központi Bank kiterjesztett eszközvásárlási programjára évekkel később, más körülmények között került sor, mint a többi vezető jegybank hasonló programjaira a 2008as pénzügyi válságot követően. A tanulmány feltárja ennek a fáziskésésnek az okait és a késedelem következményeit. Az elméletileg várt hatások, az elmúlt évek tapasztalatai és az euróövezeti sajátosságok fényében elemzi az európai mennyiségi lazítás sikerességét befolyásoló tényezőket. Sorra veszi a jegybanki lépések nem kívánt mellékhatásait, értékeli a programmal szemben ez alapján megfogalmazható kritikákat. A cikk következtetése szerint a késlekedés miatt több olyan tényezővel számolni kell, amely az eredményességre negatívan hat. Ezek miatt is kellett a jegybanki lépéseket úgy méretre szabni, hogy azok kellően hatásosak legyenek. Ez azonban egyúttal a kockázatokat is növeli és vannak hoszszabb távon érvényesülő negatív hatásai.
A kiszámíthatatlanság fokozatainak szerepe a közgazdaságtanban
Authors: Neszveda Gábor
Published: December 2020

A jövő megfelelő modellezése és az így adódó problémák elemzése mindig is a közgazdaságtan egyik központi kérdése volt. A jövő kiszámíthatatlanságának mértéke viszont eltérő lehet, attól függően, milyen modellezést alkalmazunk. A jövő kiszámíthatatlanságát a modellezők leggyakrabban „kockázatos”-nak tekintik, mégpedig azért, mert ennek a megközelítésnek vannak a legelőnyösebb matematikai tulajdonságai, miközben az is igaz, hogy ezt elfogadva kell a legerősebb feltevésekkel élnünk. Keynes is erőteljesen bírálta ezt a gyakorlatot, mivel félrevezetőnek tartotta az így kapott eredményeket. Jelen tanulmány a fogalmak bemutatása mellett arra hívja fel a figyelmet, hogy milyen hibákhoz vezethet, ha nem ismerjük a jövőt érintő főbb modellezési technikák hiányosságait. Ezen hiányosságok alapján érdemes lehet újragondolni a modelleket és azok eredményeit.