bluuebunny/crossref_metadata_2025 huggingface dataset:
Prostaglandin F2α Regulates Adipogenesis by Modulating Extracellular Signal-Regulated Kinase Signaling in Graves’ Ophthalmopathy
Authors: Zhu Ru , Wang Xing-Hua , Wang Bo-Wen , Ouyang Xuan , You Ya-Yan , Xie Hua-Tao , Zhang Ming-Chang , Jiang Fa-Gang
Published: April 2023

Prostaglandin F2α (PGF2α), the first-line anti-glaucoma medication, can cause the deepening of the upper eyelid sulcus due to orbital lipoatrophy. However, the pathogenesis of Graves’ ophthalmopathy (GO) involves the excessive adipogenesis of the orbital tissues. The present study aimed to determine the therapeutic effects and underlying mechanisms of PGF2α on adipocyte differentiation. In this study primary cultures of orbital fibroblasts (OFs) from six patients with GO were established. Immunohistochemistry, immunofluorescence, and Western blotting (WB) were used to evaluated the expression of the F-prostanoid receptor (FPR) in the orbital adipose tissues and the OFs of GO patients. The OFs were induced to differentiate into adipocytes and treated with different incubation times and concentrations of PGF2α. The results of Oil red O staining showed that the number and size of the lipid droplets decreased with increasing concentrations of PGF2α and the reverse transcription-polymerase chain reaction (RT-PCR) and WB of the peroxisome proliferator-activated receptor γ (PPARγ) and fatty-acid-binding protein 4 (FABP4), both adipogenic markers, were significantly downregulated via PGF2α treatment. Additionally, we found the adipogenesis induction of OFs promoted ERK phosphorylation, whereas PGF2α further induced ERK phosphorylation. We used Ebopiprant (FPR antagonist) to interfere with PGF2α binding to the FPR and U0126, an Extracellular Signal-Regulated Kinase (ERK) inhibitor, to inhibit ERK phosphorylation. The results of Oil red O staining and expression of adipogenic markers showed that blocking the receptor binding or decreasing the phosphorylation state of the ERK both alleviate the inhibitory effect of PGF2a on the OFs adipogenesis. Overall, PGF2α mediated the inhibitory effect of the OFs adipogenesis through the hyperactivation of ERK phosphorylation via coupling with the FPR. Our study provides a further theoretical reference for the potential application of PGF2α in patients with GO.
Combining Cryo-Thermal Therapy with Anti-IL-6 Treatment Promoted the Maturation of MDSCs to Induce Long-Term Survival in a Mouse Model of Breast Cancer
Authors: Du Peishan , Zheng Jiamin , Wang Shicheng , Lou Yue , Zhang Zelu , Wang Junjun , Zhu Yongxin , You Jiaqi , Zhang Aili , Liu Ping
Published: April 2023

Immunosuppression plays a significant role in tumor recurrence and metastasis, ultimately causing poor survival outcomes. Overcoming immunosuppression and stimulating durable antitumor immunity are essential for tumor treatment. In our previous study, a novel cryo-thermal therapy involving liquid nitrogen freezing and radiofrequency heating could reduce the proportion of Myeloid-derived suppressor cells (MDSCs), but the remaining MDSCs produced IL-6 by the NF-κB pathway, resulting in an impaired therapeutic effect. Therefore, here we combined cryo-thermal therapy with anti-IL-6 treatment to target the MDSC-dominant immunosuppressive environment, thereby optimizing the efficacy of cryo-thermal therapy. We found that combinational treatment significantly increased the long-term survival rate of breast cancer-bearing mice. Mechanistic investigation revealed that combination therapy was capable of reducing the proportion of MDSCs in the spleen and blood while promoting their maturation, which resulted in increased Th1-dominant CD4+ T-cell differentiation and enhancement of CD8+ T-mediated tumor killing. In addition, CD4+ Th1 cells promoted mature MDSCs to produce IL-7 through IFN-γ, indirectly contributing to the maintenance of Th1-dominant antitumor immunity in a positive feedback loop. Our work suggests an attractive immunotherapeutic strategy targeting the MDSC-dominant immunosuppressive environment, which would offer exciting opportunities for highly immunosuppressive and unresectable tumors in the clinic.
The Role of Mast Cells in the Induction and Maintenance of Inflammation in Selected Skin Diseases
Authors: Woźniak Ewelina , Owczarczyk-Saczonek Agnieszka , Lange Magdalena , Czarny Justyna , Wygonowska Ewa , Placek Waldemar , Nedoszytko Bogusław
Published: April 2023

Under physiological conditions, skin mast cells play an important role as guardians that quickly react to stimuli that disturb homeostasis. These cells efficiently support, fight infection, and heal the injured tissue. The substances secreted by mast cells allow for communication inside the body, including the immune, nervous, and blood systems. Pathologically non-cancerous mast cells participate in allergic processes but also may promote the development of autoinflammatory or neoplastic disease. In this article, we review the current literature regarding the role of mast cells in autoinflammatory, allergic, neoplastic skin disease, as well as the importance of these cells in systemic diseases with a pronounced course with skin symptoms.
Synergistic Effects of Silicate-Platelet Supporting Ag and ZnO, Offering High Antibacterial Activity and Low Cytotoxicity
Authors: Chang Chih-Hao , Tsai Li-Hui , Lee Yi-Chen , Yao Wei-Cheng , Lin Jiang-Jen
Published: April 2023

Silver nanoparticles (AgNPs) are remarkably able to eliminate microorganisms, but induce cytotoxicity in mammalian cells, and zinc oxide nanoparticles (ZnONPs) are considered to have a wide bactericidal effect with weak cytotoxicity. In this study, both zinc oxide nanoparticles and silver nanoparticles were co-synthesized on a nano-silicate platelet (NSP) to prepare a hybrid of AgNP/ZnONP/NSP. Ultraviolet–visible spectroscopy (UV-Vis), X-ray diffraction (XRD), and transmission electron microscopy (TEM) were used to characterize the formation of nanoparticles on the NSP. Synthesized ZnONP/NSP (ZnONP on NSP) was confirmed by the absorption peaks on UV-Vis and XRD. AgNP synthesized on ZnONP/NSP was also characterized by UV-Vis, and ZnONP/NSP showed no interference with synthesis. The images of TEM demonstrated that NSP provides physical support for the growth of nanoparticles and could prevent the inherent aggregation of ZnONP. In antibacterial tests, AgNP/ZnONP/NSP exhibited more efficacy against Staphylococcus aureus (S. aureus) than ZnONP/NSP (ZnONP was synthesized on NSP) and AgNP/NSP (AgNP was synthesized on NSP). In cell culture tests, 1/10/99 (weight ratio) of AgNP/ZnONP/NSP exhibited low cytotoxicity for mammalian cells (>100 ppm). Therefore, AgNP/ZnONP/NSP, containing both AgNP and ZnONP, with both strong antibacterial qualities and low cytotoxicity, showed potentially advantageous medical utilizations due to its antibacterial properties.
Hypoxia Increases ATX Expression by Histone Crotonylation in a HIF-2α-Dependent Manner
Authors: Qu Mengxia , Long Yang , Wang Yuqin , Yin Nan , Zhang Xiaotian , Zhang Junjie
Published: April 2023

Autotaxin (ATX), the key enzyme that generates lysophosphatidic acid (LPA) from lysophosphatidylcholine (LPC), is involved in tumorigenesis through the ATX-LPA axis and is regarded as a valuable target in tumor therapy. Hypoxia is a major feature of solid tumors and contributes to tumor development with striking alterations in the gene expression profile. Here, we show that hypoxia induces ATX expression in a hypoxia-inducible factor (HIF) 2α-dependent fashion in human colon cancer SW480 cells. HIF-2α is directly bound to specific hypoxia response elements (HREs) in the ATX promoter. Under hypoxic conditions, knockout or inhibition of ATX suppressed the migration of SW480 cells, which could be rescued by the addition of LPA, suggesting that the induction of ATX during hypoxia promotes cancer cell migration through the ATX-LPA axis. Further studies showed that ATX expression was induced by HIF-2α through recruiting p300/CBP, which led to crotonylation but not acetylation of histone H3 in the ATX promoter region during hypoxia. Moreover, elevation of cellular histone crotonylation levels could induce ATX expression under normoxic conditions. In conclusion, our findings reveal that ATX is induced in SW480 cells during hypoxia by histone crotonylation in a HIF-2α-dependent manner, while as a novel mechanism of ATX expression regulation, the upregulation of ATX expression by histone crotonylation is not confined to hypoxia.
A Biochemical Deconstruction-Based Strategy to Assist the Characterization of Bacterial Electric Conductive Filaments
Authors: Silva Marta A. , Fernandes Ana P. , Turner David L. , Salgueiro Carlos A.
Published: April 2023

Periplasmic nanowires and electric conductive filaments made of the polymeric assembly of c-type cytochromes from Geobacter sulfurreducens bacterium are crucial for electron storage and/or extracellular electron transfer. The elucidation of the redox properties of each heme is fundamental to the understanding of the electron transfer mechanisms in these systems, which first requires the specific assignment of the heme NMR signals. The high number of hemes and the molecular weight of the nanowires dramatically decrease the spectral resolution and make this assignment extremely complex or unattainable. The nanowire cytochrome GSU1996 (~42 kDa) is composed of four domains (A to D) each containing three c-type heme groups. In this work, the individual domains (A to D), bi-domains (AB, CD) and full-length nanowire were separately produced at natural abundance. Sufficient protein expression was obtained for domains C (~11 kDa/three hemes) and D (~10 kDa/three hemes), as well as for bi-domain CD (~21 kDa/six hemes). Using 2D-NMR experiments, the assignment of the heme proton NMR signals for domains C and D was obtained and then used to guide the assignment of the corresponding signals in the hexaheme bi-domain CD. This new biochemical deconstruction-based procedure, using nanowire GSU1996 as a model, establishes a new strategy to functionally characterize large multiheme cytochromes.
Casein Kinase 1α as a Novel Factor Affects Thyrotropin Synthesis via PKC/ERK/CREB Signaling
Authors: Wang Bingjie , Zhang Jinglin , Zhang Di , Lu Chenyang , Liu Hui , Gao Qiao , Niu Tongjuan , Yin Mengqing , Cui Sheng
Published: April 2023

Casein kinase 1α (CK1α) is present in multiple cellular organelles and plays various roles in regulating neuroendocrine metabolism. Herein, we investigated the underlying function and mechanisms of CK1α-regulated thyrotropin (thyroid-stimulating hormone (TSH)) synthesis in a murine model. Immunohistochemistry and immunofluorescence staining were performed to detect CK1α expression in murine pituitary tissue and its localization to specific cell types. Tshb mRNA expression in anterior pituitary was detected using real-time and radioimmunoassay techniques after CK1α activity was promoted and inhibited in vivo and in vitro. Relationships among TRH/L-T4, CK1α, and TSH were analyzed with TRH and L-T4 treatment, as well as thyroidectomy, in vivo. In mice, CK1α was expressed at higher levels in the pituitary gland tissue than in the thyroid, adrenal gland, or liver. However, inhibiting endogenous CK1α activity in the anterior pituitary and primary pituitary cells significantly increased TSH expression and attenuated the inhibitory effect of L-T4 on TSH. In contrast, CK1α activation weakened TSH stimulation by thyrotropin-releasing hormone (TRH) by suppressing protein kinase C (PKC)/extracellular signal-regulated kinase (ERK)/cAMP response element binding (CREB) signaling. CK1α, as a negative regulator, mediates TRH and L-T4 upstream signaling by targeting PKC, thus affecting TSH expression and downregulating ERK1/2 phosphorylation and CREB transcriptional activity.
Type I Diabetes in Zebrafish Reduces Sperm Quality and Increases Insulin and Glucose Transporter Transcripts
Authors: Diogo Patrícia , Martins Gil , Simão Márcio , Marreiros Ana , Eufrásio Ana Catarina , Cabrita Elsa , Gavaia Paulo Jorge
Published: April 2023

Type I diabetes is a prominent human pathology with increasing incidence in the population; however, its cause is still unknown. This disease promotes detrimental effects on reproduction, such as lower sperm motility and DNA integrity. Hence, the investigation of the underlying mechanisms of this metabolic disturbance in reproduction and its transgenerational consequences is of the utmost importance. The zebrafish is a useful model for this research considering its high homology with human genes as well as its fast generation and regeneration abilities. Therefore, we aimed to investigate sperm quality and genes relevant to diabetes in the spermatozoa of Tg(ins:nfsb-mCherry) zebrafish, a model for type I diabetes. Diabetic Tg(ins:nfsb-mCherry) males showed significantly higher expression of transcripts for insulin a (insa) and glucose transporter (slc2a2) compared to controls. Sperm obtained from the same treatment group showed significantly lower sperm motility, plasma membrane viability, and DNA integrity compared to that from the control group. Upon sperm cryopreservation, sperm freezability was reduced, which could be a consequence of poor initial sperm quality. Altogether, the data showed similar detrimental effects related to type I diabetes in zebrafish spermatozoa at the cellular and molecular levels. Therefore, our study validates the zebrafish model for type I diabetes research in germ cells.
LF Power of HRV Could Be the Piezo2 Activity Level in Baroreceptors with Some Piezo1 Residual Activity Contribution
Authors: Sonkodi Balázs
Published: April 2023

Heart rate variability is a useful measure for monitoring the autonomic nervous system. Heart rate variability measurements have gained significant demand not only in science, but also in the public due to the fairly low price and wide accessibility of the Internet of things. The scientific debate about one of the measures of heart rate variability, i.e., what low-frequency power is reflecting, has been ongoing for decades. Some schools reason that it represents the sympathetic loading, while an even more compelling reasoning is that it measures how the baroreflex modulates the cardiac autonomic outflow. However, the current opinion manuscript proposes that the discovery of the more precise molecular characteristics of baroreceptors, i.e., that the Piezo2 ion channel containing vagal afferents could invoke the baroreflex, may possibly resolve this debate. It is long known that medium- to high-intensity exercise diminishes low-frequency power to almost undetectable values. Moreover, it is also demonstrated that the stretch- and force-gated Piezo2 ion channels are inactivated in a prolonged hyperexcited state in order to prevent pathological hyperexcitation. Accordingly, the current author suggests that the almost undetectable value of low-frequency power at medium- to high-intensity exercise reflects the inactivation of Piezo2 from vagal afferents in the baroreceptors with some Piezo1 residual activity contribution. Consequently, this opinion paper highlights how low-frequency power of the heart rate variability could represent the activity level of Piezo2 in baroreceptors.
Regulatory Mechanisms of Transcription Factors in Plant Morphology and Function
Authors: Koyama Tomotsugu
Published: April 2023

Plants develop organs such as flowers and leaves with different morphologies [...]
Three-Dimensional Structural Insights Have Revealed the Distinct Binding Interactions of Agonists, Partial Agonists, and Antagonists with the µ Opioid Receptor
Authors: Li Zoe , Liu Jie , Dong Fan , Chang Nancy , Huang Ruili , Xia Menghang , Patterson Tucker A. , Hong Huixiao
Published: April 2023

The United States is experiencing the most profound and devastating opioid crisis in history, with the number of deaths involving opioids, including prescription and illegal opioids, continuing to climb over the past two decades. This severe public health issue is difficult to combat as opioids remain a crucial treatment for pain, and at the same time, they are also highly addictive. Opioids act on the opioid receptor, which in turn activates its downstream signaling pathway that eventually leads to an analgesic effect. Among the four types of opioid receptors, the µ subtype is primarily responsible for the analgesic cascade. This review describes available 3D structures of the µ opioid receptor in the protein data bank and provides structural insights for the binding of agonists and antagonists to the receptor. Comparative analysis on the atomic details of the binding site in these structures was conducted and distinct binding interactions for agonists, partial agonists, and antagonists were observed. The findings in this article deepen our understanding of the ligand binding activity and shed some light on the development of novel opioid analgesics which may improve the risk benefit balance of existing opioids.
Studies on the Accumulation of Secondary Metabolites and Evaluation of Biological Activity of In Vitro Cultures of Ruta montana L. in Temporary Immersion Bioreactors
Authors: Szewczyk Agnieszka , Marino Andreana , Taviano Maria Fernanda , Cambria Lucia , Davì Federica , Trepa Monika , Grabowski Mariusz , Miceli Natalizia
Published: April 2023

The present work focuses on in vitro cultures of Ruta montana L. in temporary immersion PlantformTM bioreactors. The main aim of the study was to evaluate the effects of cultivation time (5 and 6 weeks) and different concentrations (0.1–1.0 mg/L) of plant growth and development regulators (NAA and BAP) on the increase in biomass and the accumulation of secondary metabolites. Consequently, the antioxidant, antibacterial, and antibiofilm potentials of methanol extracts obtained from the in vitro-cultured biomass of R. montana were evaluated. High-performance liquid chromatography analysis was performed to characterize furanocoumarins, furoquinoline alkaloids, phenolic acids, and catechins. The major secondary metabolites in R. montana cultures were coumarins (maximum total content of 1824.3 mg/100 g DM), and the dominant compounds among them were xanthotoxin and bergapten. The maximum content of alkaloids was 561.7 mg/100 g DM. Concerning the antioxidant activity, the extract obtained from the biomass grown on the 0.1/0.1 LS medium variant, with an IC50 0.90 ± 0.03 mg/mL, showed the best chelating ability among the extracts, while the 0.1/0.1 and 0.5/1.0 LS media variants showed the best antibacterial (MIC range 125–500 µg/mL) and antibiofilm activity against resistant Staphylococcus aureus strains.
Glioblastoma Microenvironment and Invasiveness: New Insights and Therapeutic Targets
Authors: Erices José Ignacio , Bizama Carolina , Niechi Ignacio , Uribe Daniel , Rosales Arnaldo , Fabres Karen , Navarro-Martínez Giovanna , Torres Ángelo , San Martín Rody , Roa Juan Carlos , Quezada-Monrás Claudia
Published: April 2023

Glioblastoma (GBM) is the most common and malignant primary brain cancer in adults. Without treatment the mean patient survival is approximately 6 months, which can be extended to 15 months with the use of multimodal therapies. The low effectiveness of GBM therapies is mainly due to the tumor infiltration into the healthy brain tissue, which depends on GBM cells’ interaction with the tumor microenvironment (TME). The interaction of GBM cells with the TME involves cellular components such as stem-like cells, glia, endothelial cells, and non-cellular components such as the extracellular matrix, enhanced hypoxia, and soluble factors such as adenosine, which promote GBM’s invasiveness. However, here we highlight the role of 3D patient-derived glioblastoma organoids cultures as a new platform for study of the modeling of TME and invasiveness. In this review, the mechanisms involved in GBM-microenvironment interaction are described and discussed, proposing potential prognosis biomarkers and new therapeutic targets.
The Effects of Learning and Eating Behaviours among Medical Students during the COVID-19 Pandemic
Authors: Rahman Sayeeda , Kirton Rita , Roach Brenda , Montero Maria Teresa Villagomez , Podcheko Alexey , Nouraee Nader , Ahsan Sadia , Nisar Joshua Noel , Rabbi Ahbab Mohammad Fazle
Published: April 2023

Background: The effect of the COVID-19 pandemic has transformed medical education and is likely to have long-lasting effects on student learning, mental well-being, and eating behaviour. This study aimed to examine the learning behaviours of medical students at the American University of Integrative Sciences (AUIS), Barbados, during the COVID-19 pandemic. Methods: A cross-sectional web-based on-line survey was administered to medical students at AUIS from July to November 2021. The data collecting instrument recorded students’ demographic and learning behaviour information and eating disorders (SCOFF questionnaire). Results: The overall response rate was 55% (n = 120). In relation to learning behaviour, students agreed with the following statements: ‘deterioration in work performance and studying’ (48.4%), ‘remember subject’s contents appropriately’ (40.4%), ‘concentration on the studies’ (40.3%), ‘difficulty in performing two tasks simultaneously’ (38.7%), ‘difficulty in performing mental calculations’ (33.9%), ‘difficulty in recalling recent information’ (32.3%), and ‘difficulty in recalling old information’ (38.7%). Among the eight dimensions of learning behaviours, deterioration in work performance or studying and difficulties in recalling recent information were found to be significantly associated with the gender of the students. For the SCOFF questionnaire, approximately 24.2% screened positive for eating disorders. Screening with the SCOFF test demonstrated that females, older (>25 years), overweight + obese, Clinical Sciences + PreMed, and non-USA-based students were at more risk of eating disorders. Conclusions: The results indicate that during the COVID-19 pandemic, AUIS students have developed learning difficulties and are likely to have eating disorders. University policymakers should take appropriate measures to support a healthy learning environment and improve students’ mental well-being and eating behaviours.
Urinary Incontinence in Parous Women Practicing Non-Extreme Competitive Sports Compared to the General Population
Authors: Ben Zvi Masha , Arad Cohen Maya , Friedman Matan , Ganer Herman Hadas , Weiner Eran , Ginath Shimon
Published: April 2023

Introduction and objective: This cross-sectional study aimed to compare the prevalence of urinary symptoms in physically active females to the general population represented by medical staff. Materials and methods: We conducted a survey utilizing the UDI-6 questionnaire on women playing catchball for at least one year and training twice a week or more in an official Israeli competitive league. The control group consisted of women practicing medicine (physicians and nurses). Results: The study group consisted of 317 catchball players and the control group consisted of 105 medical staff practitioners. Both groups were similar in most of the demographic characteristics. Urinary symptoms represented by UDI-6 scores were higher in women in the catchball group. Frequency and urgency symptoms were common in women playing catchball. Stress urinary incontinence (SUI) was insignificant between the groups (43.8% in the catchball group and 35.2% in the medical staff group, p = 0.114). However, severe symptoms of SUI were more common in catchball players. Conclusions: The rates of all urinary symptoms were higher in in catchball players. SUI symptoms were common in both groups. However, severe symptoms of SUI were more common in catchball players.
The Beneficial Effect of Preoperative Exercise on Postoperative Clinical Outcome, Quality of Life and Return to Work after Microsurgical Resection of Spinal Meningiomas
Authors: Kilinc Fatma , Setzer Matthias , Prinz Vincent , Jussen Daniel , Marquardt Gerhard , Gessler Florian , Czabanka Marcus , Freiman Thomas , Dubinski Daniel , Won Sae-Yeon , Haberland Moritz , Behmanesh Bedjan
Published: April 2023

Objective: While outcomes of surgical treatment for spinal meningiomas are well-described within the literature, factors affecting early return to work as well as long-term health related quality of life remain unclear. Methods: In this retrospective study, patients with spinal meningioma and surgical treatment from two university-level neurosurgical institutions between 2008 and 2021 were analyzed. Time to return to work, physical activities and long-term health related quality of life (assessed by telephone interviews using the EQ-5D-5L health status measure and visual analogue scale (EQ VAS) were analyzed. Results: We identified a total of 196 patients who underwent microsurgical resection of spinal meningioma between January 2008 and December 2021. Of those, 130 patients of working age were included and analyzed. The median follow-up time was 96 months. All included patients returned to work. The median time of return to work was 45 days for the whole cohort. Patients who preoperatively performed physical activity returned to work significantly earlier compared to patients who did not (p < 0.001). Furthermore, younger age (p = 0.033) and absence of obesity (p = 0.023) correlated significantly with earlier return to work. Significant differences were also observed in all 5 EQ-5D-5L dimensions between patients with and without preoperative physical activity. Conclusions: Despite the benign nature of spinal meningioma preoperative physical activity and physiological body weight are associated with favorable postoperative outcome, higher quality of life and early return to work.
Impacts of Structure-Directing Agents on the Synthesis of Cu3Mo2O9 for Flexible Lignin-Based Supercapacitor Electrodes
Authors: Mehta Siddhi , Kumaravel Sangeetha , Jha Swarn , Yen Matthew , Kundu Subrata , Liang Hong
Published: April 2023

Due to demands for sustainability, the interest in energy storage devices constructed from green materials has increased immensely. These devices currently have yet to be satisfactory. Issues include high production costs and toxicity, limited dependability, and subpar electrochemical performance. In this research, low-cost, plant-based electroactive Cu3Mo2O9 materials were synthesized via co-precipitation followed by an annealing method using two different structure-directing agents, i.e., the commonly used surfactant cetyltrimethylammonium bromide (CTAB) and the biomolecule deoxyribonucleic acid (DNA) as a greener alternative, and these materials were studied for the first time. Further, the Cu3Mo2O9 nanoparticles developed using CTAB and DNA were integrated into the lignin matrix and studied as flexible electrodes for supercapacitor application. Here, the morphological advantages of the nanorods and nanosheets formed by varying the synthesis methods and their effects during supercapacitor studies were studied in detail. After 1200 cycles, the Al/lig-Cu3Mo2O9@DNA supercapacitor exhibited higher capacitive performance when compared to the Al/lig-Cu3Mo2O9@CTAB supercapacitor. The Al/Lig-Cu3Mo2O9@DNA supercapacitor had an initial specific capacitance of 404.64 mF g−1 with a ~70% retention, while the Al/Lig-Cu3Mo2O9@CTAB supercapacitor had an initial specific capacitance of 309.59 mF g−1 with a ~50% retention. This study offers a new approach to creating scalable, low-cost, green composite CuMoO4-based electrodes for flexible supercapacitors.
Punctiform Breakup and Initial Oceanization in the Central Red Sea Rift
Authors: Sang Ya-Di , Adam Bakhit M. T. , Li Chun-Feng , Huang Liang , Wen Yong-Lin , Zhang Jia-Ling , Liu Yu-Tao
Published: April 2023

The Central Red Sea Rift is a natural laboratory to study the transition from rifting to spreading. Based on new reflection seismic profiles and gravity modeling, we examined the crustal structure, tectonic evolution, breakup mechanism, and future evolution of the Central Red Sea Rift. Along this rift axis, the breakup of continental lithosphere is discontinuous and the oceanic crust is limited to the axial deeps. The punctiform breakup and formation of deeps is assisted by mantle upwelling and topographic uplift, but the nucleation is directly controlled by the normal-fault system. The discontinuities spaced between axial deeps within the relatively continuous central troughs are presently axial domes or highs and will evolve into new deeps with tectonic subsidence. Isolated deeps will grow and connect with each other to become a continuous central trough, before transitioning into a unified spreading center.
The Impact of Changes in the Marine Environment on Marine Organisms
Authors: Hsieh Hung-Yen , Tew Kwee-Siong , Meng Pei-Jie
Published: April 2023

The ocean is a critical element of human well-being and livelihood, providing a home for a diverse range of creatures that inhabit both coastal and pelagic ecosystems, from microbes to marine mammals [...]
The Importance of Lifestyle Interventions in the Prevention and Treatment of Chronic Kidney Disease
Authors: Castle Ellen M. , Greenwood Sharlene A. , Müller Roman-Ulrich
Published: April 2023

Chronic kidney disease (CKD) is a global health problem, with a prevalence of approximately 13 [...]
Composite Cold-Formed Steel Beams with Diagonal Rebars for Earthquake-Resistant Buildings
Authors: Samuel James , Nair Shalini Ramachandran , Joanna Philip Saratha , Gurupatham Beulah Gnana Ananthi , Roy Krishanu , Lim James Boon Piang
Published: April 2023

The construction industry is on the lookout for cost-effective structural members that are also environmentally friendly. Built-up cold-formed steel (CFS) sections with minimal thickness can be used to make beams at a lower cost. Plate buckling in CFS beams with thin webs can be avoided by using thick webs, adding stiffeners, or strengthening the web with diagonal rebars. When CFS beams are designed to carry heavy loads, their depth logically increases, resulting in an increase in building floor height. The experimental and numerical investigation of CFS composite beams reinforced with diagonal web rebars is presented in this paper. A total of twelve built-up CFS beams were used for testing, with the first six designed without web encasement and the remaining six designed with web encasement. The first six were constructed with diagonal rebars in the shear and flexure zones, while the other two with diagonal rebars in the shear zone, and the last two without diagonal rebars. The next set of six beams was constructed in the same manner, but with a concrete encasement of the web, and all the beams were then tested. Fly ash, a pozzolanic waste byproduct of thermal power plants, was used as a 40% replacement for cement in making the test specimens. CFS beam failure characteristics, load–deflection behavior, ductility, load–strain relationship, moment–curvature relationship, and lateral stiffness were all investigated. The results of the experimental tests and the nonlinear finite element analysis performed in ANSYS software were found to be in good agreement. It was discovered that CFS beams with fly ash concrete encased webs have twice the moment resisting capacity of plain CFS beams, resulting in a reduction in building floor height. The results also confirmed that the composite CFS beams have high ductility, making them a reliable choice for earthquake-resistant structures.
Strengthening Mechanism of Rotary-Forged Deformable Biodegradable Zn-0.45Li Alloys
Authors: Ding Feng , Zhang Yi , Zhu Xinglong , Guo Pushan , Yang Lijing , Zhang Qingke , Xu Cheng , Sun Wensheng , Song Zhenlun
Published: April 2023

The use of zinc (Zn) alloys as a biodegradable metal for medical purposes has been a popular research topic. This study investigated the strengthening mechanism of Zn alloys to enhance their mechanical properties. Three Zn-0.45Li (wt.%) alloys with different deformation amounts were prepared by rotary forging deformation. Their mechanical properties and microstructures were tested. A simultaneous increase in strength and ductility was observed in the Zn-0.45Li alloys. Grain refinement occurred when the rotary forging deformation reached 75.7%. The surface average grain size reached 1.19 ± 0.31 μm, and the grain size was uniformly distributed. Meanwhile, the maximum elongation of the deformed Zn-0.45Li was 139.2 ± 18.6%, and the ultimate tensile strength reached 426.1 ± 4.7 MPa. In situ tensile tests showed that the reinforced alloys still broke from the grain boundary. Continuous and discontinuous dynamic recrystallization during severe plastic deformation produced many recrystallized grains. During deformation, the dislocation density of the alloy first increased and then decreased, and the texture strength of the (0001) direction increased with deformation. Analysis of the mechanism of alloy strengthening showed that the strength and plasticity enhancement of Zn-Li alloys after macro deformation was a combination of dislocation strengthening, weave strengthening, and grain refinement rather than only fine-grain strengthening as observed in conventional macro-deformed Zn alloys.
A Novel Approach of Optimum Time Interval Estimation for Al-7.5Si/Al-18Si Liquid–Liquid Bimetal Casting in Sand and Metallic Moulds
Authors: Fathy Naglaa , Ramadan Mohamed , Hafez Khalid M. , Abdulaziz Fahad , Ayadi Badreddine , Alghamdi Abdulaziz S.
Published: April 2023

This work describes a novel approach for Al-7.5Si/Al-18Si liquid–liquid bimetal casting in sand and metallic moulds. The aim of the work is to facilitate and develop a simple procedure to produce an Al-7.5Si/Al-18Si bimetallic material with a smooth gradient interface structure. The procedure involves the theoretical calculation of total solidification time (TST) of the first liquid metal (M1), pouring the liquid metal (M1), and allowing it to solidify; then, before complete solidification, the second liquid metal (M2) is introduced into the mould. This novel approach has been proven to produce Al-7.5Si/Al-18Si bimetal materials using liquid–liquid casting. The optimum time interval of Al-7.5Si/Al-18Si bimetal casting with modulus of cast Mc ≤ 1 was estimated based on subtracting 5–15 s or 1–5 s from TST of M1 for sand and metallic moulds, respectively. Future work will involve determining the appropriate time interval range for castings having modulus ≥ 1 using the current approach.
Effects of Post-Weld Heat Treatment on Microstructure and Mechanical Properties of the Brazed Joint of a Novel Fourth-Generation Nickel-Based Single Crystal Superalloy
Authors: Zhang Zhipeng , Liu Jide , Zhu Chongwei , Huang Yuyu , Wang Xinguang , Zhou Yizhou , Wang Jianjun , Li Jinguo
Published: April 2023

A novel fourth-generation nickel-based single crystal superalloy was brazed with Co-based filler alloy. The effects of post-weld heat treatment (PWHT) on the microstructure and mechanical properties of brazed joints were investigated. The experimental and CALPHAD simulation results show that the non-isothermal solidification zone was composed of M3B2, MB-type boride and MC carbide, and the isothermal solidification zone was composed of γ and γ’ phases. After the PWHT, the distribution of borides and the morphology of the γ’ phase were changed. The change of the γ’ phase was mainly attributed to the effect of borides on the diffusion behavior of Al and Ta atoms. In the process of PWHT, stress concentration leads to the nucleation and growth of grains during recrystallization, thus forming high angle grain boundaries in the joint. The microhardness was slightly increased compared to the joint before PWHT. The relationship between microstructure and microhardness during the PWHT of the joint was discussed. In addition, the tensile strength and stress fracture life of the joints were significantly increased after the PWHT. The reasons for the improved mechanical properties of the joints were analyzed and the fracture mechanism of the joints was elucidated. These research results can provide important guidance for the brazing work of fourth-generation nickel-based single crystal superalloy.
Sustainable Lifecycle of Perforated Metal Materials
Authors: Mironovs Viktors , Kuzmina Jekaterina , Serdjuks Dmitrijs , Usherenko Yulia , Lisicins Mihails
Published: April 2023

In an era of rapidly growing consumer demand and the subsequent development of production, light materials and structures with a wide range of applications are becoming increasingly important in the field of construction and mechanical engineering, including aerospace engineering. At the same time, one of the trends is the use of perforated metal materials (PMMs). They are used as finishing, decorative and structural building materials. The main feature of PMMs is the presence of through holes of a given shape and size, which makes it possible to have low specific gravity; however, their tensile strength and rigidity can vary widely depending on the source material. In addition, PMMs have several properties that cannot be achieved with solid materials; for example, they can provide considerable noise reduction and partial light absorption, significantly reducing the weight of structures. They are also used for damping dynamic forces, filtering liquids and gases and shielding electromagnetic fields. For the perforation of strips and sheets, cold stamping methods are usually used, carried out on stamping presses, particularly using wide-tape production lines. Other methods of manufacturing PMMs are rapidly developing, for example, using liquid and laser cutting. An urgent but relatively new and little-studied problem is the recycling and further efficient use of PMMs, primarily such materials as stainless and high-strength steels, titanium, and aluminum alloys. The life cycle of PMMs can be prolonged because they can be repurposed for various applications such as constructing new buildings, designing elements, and producing additional products, making them more environmentally friendly. This work aimed to overview sustainable ways of PMM recycling, use or reuse, proposing different ecological methods and applications considering the types and properties of PMM technological waste. Moreover, the review is accompanied by graphical illustrations of real examples. PMM waste recycling methods that can prolong their lifecycle include construction technologies, powder metallurgy, permeable structures, etc. Several new technologies have been proposed and described for the sustainable application of products and structures based on perforated steel strips and profiles obtained from waste products during stamping. With more developers aiming for sustainability and buildings achieving higher levels of environmental performance, PMM provides significant environmental and aesthetic advantages.
The Dynamical Behavior of a Three-Dimensional System of Exponential Difference Equations
Authors: Khaliq Abdul , Sadiq Stephen , Ahmed Hala M. E. , Mahmoud Batul A. A. , Al-Sinan Bushra R. , Ibrahim Tarek Fawzi
Published: April 2023

The boundedness nature and persistence, global and local behavior, and rate of convergence of positive solutions of a second-order system of exponential difference equations, is investigated in this work. Where the parameters A,B,C,α,β,γ,δ,η, and ξare constants that are positive, and the initials U−1,U0,V−1,V0,W−1, and W0 are non-negative real numbers. Some examples are provided to support our theoretical results.
Ferroptosis Inhibitory Compounds from the Deep-Sea-Derived Fungus Penicillium sp. MCCC 3A00126
Authors: Hao You-Jia , Zou Zheng-Biao , Xie Ming-Min , Zhang Yong , Xu Lin , Yu Hao-Yu , Ma Hua-Bin , Yang Xian-Wen
Published: April 2023

Two new xanthones (1 and 2) were isolated from the deep-sea-derived fungus Penicillium sp. MCCC 3A00126 along with 34 known compounds (3–36). The structures of the new compounds were established by spectroscopic data. The absolute configuration of 1 was validated by comparison of experimental and calculated ECD spectra. All isolated compounds were evaluated for cytotoxicity and ferroptosis inhibitory activities. Compounds 14 and 15 exerted potent cytotoxicity against CCRF-CEM cells, with IC50 values of 5.5 and 3.5 μM, respectively, whereas 26, 28, 33, and 34 significantly inhibited RSL3-induced ferroptosis, with EC50 values of 11.6, 7.2, 11.8, and 2.2 μM, respectively.
Pyrite Textures and Compositions in the Dunbasitao Gold Deposit, NW China: Implications for Ore Genesis and Gold Mineralization Processes
Authors: Liu Wenxiang , Deng Xiaohua , Han Shen , Chen Xi , Li Xun , Aibai Abulimiti , Wu Yanshuang , Wang Yong , Shan Wei , Li Zengsheng , Chen Yanjing
Published: April 2023

The process and mechanism of gold mineralization are frontier issues. The Dunbasitao deposit is the most important gold deposit discovered along the Armantai suture zone, East Junggar, NW China, which indicates the potential for future ore exploration in this area. Orebodies are mainly hosted in Lower Carboniferous Jiangbasitao Formation volcano-sedimentary rocks, and the ores are characterized by multistage pyrites. Based on microscopy and backscattered electron imaging studies, pyrites are classified into five types: the pre-ore framboidal/colloidal Py0; the early-stage coarse-grained, cubic, and homogeneous Py1; and the middle-stage fine-grained, cubic/pyritohedron Py2 that includes Py2a (core), Py2b (mantle), and Py2c (rim). The results of the EPMA and in situ LA-ICP-MS analyses show that trace elements of pyrite mainly occur in two forms: solid solutions and invisible or visible inclusions. Mn, Co, Ni, and As enter the pyrite lattice, whereas Ti occurs as mineral inclusions, and Au, Cu, Zn, Sb, and Pb can occur in both forms. Au and As show a positive linear relationship with r = 0.850. Py2b has much higher Au contents (20.1 to 201 ppm) than other pyrite types (Py0: 0.01 to 0.36 ppm; Py1: 0.01 to 0.02 ppm; Py2a: 0.31 to 2.48 ppm; and Py2c: 0.18 to 18.0 ppm). The Dunbasitao deposit is classified as an orogenic gold deposit using the two latest machine learning classifiers based on pyrite trace element data. Fluid immiscibility, sudden cooling, and the substitution of S1− with As1− might be crucial mechanisms leading to Au precipitation. Initial ore-forming fluids brought major amounts of As, Au, Co, Ni, Se, Zn, Ag, Cd, Sn, and other elements, and the Jiangbasitao Formation host rocks contributed a certain amount of As, Ni, Cu, Sb, Pb, and Bi, at least.
In Vitro: The Effects of the Anticoccidial Activities of Calotropis procera Leaf Extracts on Eimeria stiedae Oocysts Isolated from Rabbits
Authors: Murshed Mutee , Aljawdah Hossam M. A. , Mares Mohammed , Al-Quraishy Saleh
Published: April 2023

Hepatic coccidiosis is an infectious and mortal disease that causes global economic losses in rabbits. The research aimed to assess the efficacy of Calotropis procure leaf extracts on the inhibition of Eimeria stiedae oocysts and to determine the optimal dosage for suppressing the parasite’s infective phase. In this experiment, oocyst samples per milliliter were tested, and 6-well plates (2 mL) of 2.5% potassium dichromate solution containing 102 non-sporulated oocysts on Calotropis procera leaf extracts were exposed after 24, 48, 72, and 96 h, and the treatments were as follows: a nontreated control, 25%, 50%, 100%, and 150% of C. procera for oocyst activities. In addition, amprolium was utilized as a reference drug. The Calotropis procera was analyzed by GC-Mass, and results showed that the botanical extract contained 9 chemical components that were able to inhibit the oocysts of E. stiedae at 100% and 150% concentrations by about 78% and 93%, respectively. In general, an increase in the incubation period and a greater dose resulted in a decrease in the inhibition rate. The results showed that C. procera has an effective ability, inhibitory potential, and protective effect on the coccidian oocyst sporulation of E. stiedae. It can be used in the disinfection and sterilization of poultry and rabbit houses to get rid of Eimeria oocysts.
Computational Exploration of Licorice for Lead Compounds against Plasmodium vivax Duffy Binding Protein Utilizing Molecular Docking and Molecular Dynamic Simulation
Authors: Yasir Muhammad , Park Jinyoung , Han Eun-Taek , Park Won Sun , Han Jin-Hee , Kwon Yong-Soo , Lee Hee-Jae , Chun Wanjoo
Published: April 2023

Plasmodium vivax (P. vivax) is one of the human’s most common malaria parasites. P. vivax is exceedingly difficult to control and eliminate due to the existence of extravascular reservoirs and recurring infections from latent liver stages. Traditionally, licorice compounds have been widely investigated against viral and infectious diseases and exhibit some promising results to combat these diseases. In the present study, computational approaches are utilized to study the effect of licorice compounds against P. vivax Duffy binding protein (DBP) to inhibit the malarial invasion to human red blood cells (RBCs). The main focus is to block the DBP binding site to Duffy antigen receptor chemokines (DARC) of RBC to restrict the formation of the DBP–DARC complex. A molecular docking study was performed to analyze the interaction of licorice compounds with the DARC binding site of DBP. Furthermore, the triplicates of molecular dynamic simulation studies for 100 ns were carried out to study the stability of representative docked complexes. The leading compounds such as licochalcone A, echinatin, and licochalcone B manifest competitive results against DBP. The blockage of the active region of DBP resulting from these compounds was maintained throughout the triplicates of 100 ns molecular dynamic (MD) simulation, maintaining stable hydrogen bond formation with the active site residues of DBP. Therefore, the present study suggests that licorice compounds might be good candidates for novel agents against DBP-mediated RBC invasion of P. vivax.
Seed Priming with Single-Walled Carbon Nanotubes Grafted with Pluronic P85 Preserves the Functional and Structural Characteristics of Pea Plants
Authors: Krumova Sashka , Petrova Asya , Petrova Nia , Stoichev Svetozar , Ilkov Daniel , Tsonev Tsonko , Petrov Petar , Koleva Dimitrina , Velikova Violeta
Published: April 2023

The engineering of carbon nanotubes in the last decades resulted in a variety of applications in electronics, electrochemistry, and biomedicine. A number of reports also evidenced their valuable application in agriculture as plant growth regulators and nanocarriers. In this work, we explored the effect of seed priming with single-walled carbon nanotubes grafted with Pluronic P85 polymer (denoted P85-SWCNT) on Pisum sativum (var. RAN-1) seed germination, early stages of plant development, leaf anatomy, and photosynthetic efficiency. We evaluated the observed effects in relation to hydro- (control) and P85-primed seeds. Our data clearly revealed that seed priming with P85-SWCNT is safe for the plant since it does not impair the seed germination, plant development, leaf anatomy, biomass, and photosynthetic activity, and even increases the amount of photochemically active photosystem II centers in a concentration-dependent manner. Only 300 mg/L concentration exerts an adverse effect on those parameters. The P85 polymer, however, was found to exhibit a number of negative effects on plant growth (i.e., root length, leaf anatomy, biomass accumulation and photoprotection capability), most probably related to the unfavorable interaction of P85 unimers with plant membranes. Our findings substantiate the future exploration and exploitation of P85-SWCNT as nanocarriers of specific substances promoting not only plant growth at optimal conditions but also better plant performance under a variety of environmental stresses.