bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on Forest and Grassland Ecological Space Structure in Eyu Mining Area and Potential Alternatives for Enhancing Carbon Sequestration
Authors: Wang Ge , Yue Depeng , Yu Qiang , Yang Di , Xu Chenglong , Wang Fei
Published: August 2023

Optimizing the connectivity-carbon sequestration coupling coordination of forest and grassland ecological spaces (F&GES) is a crucial measure to enhance carbon sequestration effectively in mining areas. However, the prevailing strategies for optimizing F&GES often overlook the connectivity-carbon sequestration coupling coordination of the network. Therefore, this study aimed to propose a novel restoration plan to improve the connectivity-carbon sequestration coupling coordination of existing networks. Taking a typical mining area in northwestern China (Eyu County) as an example, we extracted the existing F&GES based on remote sensing ecological indicators and ecological risk assessments. Subsequently, we optimized the network using the connectivity-carbon sequestration coupling coordination degree (CSCCD) model from the perspective of connectivity-carbon sequestration coupling coordination, proposed potential alternative optimization schemes, and evaluated the optimization effects. The results showed that the range of Eyu County’s F&GES structure had been determined. Ecological source sites with better carbon sequestration effects were primarily distributed in the central and northeastern parts of Eyu County. After optimization, the network added 26 ecological patches, and the added area reached 641.57 km2. Furthermore, the connectivity robustness, edge restoration robustness, and node restoration robustness of the optimized network were significantly improved, and the carbon sequestration effect of the forest and grassland ecological space was increased by 6.78%. The contribution rate of ecological source sites was 97.66%, and that of ecological corridors was 2.34%. The CSCCD model proposed in this study can effectively improve the carbon sequestration effect in mining areas, promote carbon neutrality, and save network optimization time while improving efficiency. This restoration strategy is also applicable to forest and grassland ecosystem management and optimization of ecological spaces in other mining areas, which has positive implications for promoting ecological civilization construction and sustainable development.
The Influence of Socioeconomic Factors on Human Wildfire Ignitions in the Pacific Northwest, USA
Authors: Reilley Caitlyn , Crandall Mindy S. , Kline Jeffrey D. , Kim John B. , de Diego Jaime
Published: August 2023

Historical land and fire management practices coupled with climate change and modern human development pressures are contributing to larger, more frequent, and more severe wildfires across Western U.S. forests. Human ignitions are the predominant cause of wildfire throughout the United States, necessitating wildfire management strategies that consider both the causes of human ignitions and the factors that influence them. Using a dataset of over 104,000 ignitions from 1992 to 2018 for Oregon and Washington (U.S), we examine the major causes of wildfire ignitions and build regression models to evaluate the potential influence of both biophysical and socioeconomic factors on human and natural ignitions across distinct fire regimes west and east of the Cascade Range. Our results corroborate prior findings that socioeconomic factors such as income, employment, population density, and age demographics are significantly correlated with human ignitions. In the Pacific Northwest, we found that the importance of socioeconomic factors on human ignitions differs significantly between the west and east sides of the Cascade Range. We also found that most human ignitions are linked to escaped fires from recreation or debris and open burning activities, highlighting opportunities to tailor wildfire prevention efforts to better control higher risk activities and reduce accidental ignitions.
Investigating the Effects of a Fractional Operator on the Evolution of the ENSO Model: Bifurcations, Stability and Numerical Analysis
Authors: Zhang Yuqi , Li Peiluan , Xu Changjin , Peng Xueqing , Qiao Rui
Published: August 2023

Recent years have seen an increase in scientific interest in the El Nio/La Nia Southern Oscillation (ENSO), a quasiperiodic climate phenomenon that takes place throughout the tropical Pacific Ocean over five years and causes significant harm. It is associated with the warm oceanic stage known as El Nio and the cold oceanic stage known as La Nia. In this research, the ENSO model is considered under a fractional operator, which is defined via a nonsingular and nonlocal kernel. Some theoretical features, such as equilibrium points and their stability, bifurcation maps, the existence of a unique solution via the Picard–Lindelof approach, and the stability of the solution via the Ulam–Hyres stability approach, are deliberated for the proposed ENSO model. The Adams–Bashforth numerical method, associated with Lagrangian interpolation, is used to obtain a numerical solution for the considered ENSO model. The complex dynamics of the ENSO model are displayed for a few fractional orders via MATLAB-18.
Correction: de Almeida et al. Acute Promyelocytic Leukemia (APL): A Review of the Classic and Emerging Target Therapies towards Molecular Heterogeneity. Future Pharmacol. 2023, 3, 162–179
Authors: de Almeida Tâmara Dauare , Evangelista Fernanda Cristina Gontijo , Sabino Adriano de Paula
Published: August 2023

In the original publication [...]
Socio-Economic Factors Associated with Ethnic Disparities in SARS-CoV-2 Infection and Hospitalization
Authors: Gili Alessio , Caminiti Marta , Lupi Chiara , Zichichi Salvatore , Minicucci Ilaria , Pezzotti Patrizio , Primieri Chiara , Bietta Carla , Stracci Fabrizio
Published: August 2023

Background: There is evidence that non-Italians presented higher incidence of infection and worse health outcomes if compared to native populations in the COVID-19 pandemic. The aim of the study was to compare Italian- and non-Italian-born health outcomes, accounting for socio-economic levels. Methods: We analyzed data relative to 906,463 people in Umbria (Italy) from 21 February 2020 to 31 May 2021. We considered the National Deprivation Index, the Urban–Rural Municipalities Index and the Human Development Index (HDI) of the country of birth. We used a multilevel logistic regression model to explore the influence of these factors on SARS-CoV-2 infection and hospitalization rates. Diagnosis in the 48 h preceding admission was an indicator of late diagnosis among hospitalized cases. Results: Overall, 54,448 persons tested positive (6%), and 9.7% of them were hospitalized. The risk of hospital admission was higher among non-Italians and was inversely related to the HDI of the country of birth. A diagnosis within 48 h before hospitalization was more frequent among non-Italians and correlated to the HDI level. Conclusions: COVID-19 had unequal health outcomes among the population in Umbria. Reduced access to primary care services in the non-Italian group could explain our findings. Policies on immigrants' access to primary healthcare need to be improved.
The Trophoblast Compartment Helps Maintain Embryonic Pluripotency and Delays Differentiation towards Cardiomyocytes
Authors: Zhao Xiang , Radford Bethany N. , Ungrin Mark , Dean Wendy , Hemberger Myriam
Published: August 2023

Normal developmental progression relies on close interactions between the embryonic and extraembryonic lineages in the pre- and peri-gastrulation stage conceptus. For example, mouse epiblast-derived FGF and NODAL signals are required to maintain a stem-like state in trophoblast cells of the extraembryonic ectoderm, while visceral endoderm signals are pivotal to pattern the anterior region of the epiblast. These developmental stages also coincide with the specification of the first heart precursors. Here, we established a robust differentiation protocol of mouse embryonic stem cells (ESCs) into cardiomyocyte-containing embryoid bodies that we used to test the impact of trophoblast on this key developmental process. Using trophoblast stem cells (TSCs) to produce trophoblast-conditioned medium (TCM), we show that TCM profoundly slows down the cardiomyocyte differentiation dynamics and specifically delays the emergence of cardiac mesoderm progenitors. TCM also strongly promotes the retention of pluripotency transcription factors, thereby sustaining the stem cell state of ESCs. By applying TCM from various mutant TSCs, we further show that those mutations that cause a trophoblast-mediated effect on early heart development in vivo alter the normal cardiomyocyte differentiation trajectory. Our approaches provide a meaningful deconstruction of the intricate crosstalk between the embryonic and the extraembryonic compartments. They demonstrate that trophoblast helps prolong a pluripotent state in embryonic cells and delays early differentiative processes, likely through production of leukemia inhibitory factor (LIF). These data expand our knowledge of the multifaceted signaling interactions among distinct compartments of the early conceptus that ensure normal embryogenesis, insights that will be of significance for the field of synthetic embryo research.
A Potential Association between Ribonuclease 1 Dynamics in the Blood and the Outcome in COVID-19 Patients
Authors: Zechendorf Elisabeth , Beckers Christian , Frank Nadine , Kraemer Sandra , Neu Carolina , Breuer Thomas , Dreher Michael , Dahl Edgar , Marx Gernot , Martin Lukas , Simon Tim-Philipp
Published: August 2023

The COVID-19 pandemic caused by the new SARS-CoV-2 coronavirus is the most recent and well-known outbreak of a coronavirus. RNase 1 is a small endogenous antimicrobial polypeptide that possesses antiviral activity against viral diseases. In this study, we investigated a potential association between ribonuclease 1 and the outcome in COVID-19 patients and the impact of increased and decreased RNase 1 levels serum during the course of the disease. Therefore, two patient populations, Cohort A (n = 35) and B (n = 80), were subclassified into two groups, in which the RNase 1 concentration increased or decreased from time point one to time point two. We show that the RNase 1 serum levels significantly increased in the increasing group of both cohorts (p = 0.0171; p < 0.0001). We detect that patients in the increasing group who died had significantly higher RNase 1 serum levels at both time points in Cohort A (p = 0.0170; p = 0.0393) and Cohort B (p = 0.0253; p = 0.0034) than patients who survived. Additionally, we measured a significant correlation of RNase 1 serum levels with serum creatinine as well as creatinine clearance in the increasing and decreasing group at both time points of Cohort A. Based on these results, there is now good evidence that RNase 1 may play a role in renal dysfunction associated with ICU COVID-19 patients and that increasing RNase 1 serum level may be a potential biomarker to predict outcome in COVID-19 patients.
Impact of Somatic DNA Repair Mutations on the Clinical Outcomes of Bone Metastases from Castration-Resistant Prostate Cancer
Authors: Cursano Maria Concetta , Giunta Emilio Francesco , Scarpi Emanuela , Casadei Chiara , Virga Alessandra , Ulivi Paola , Bleve Sara , Brighi Nicole , Ravaglia Giorgia , Pantano Francesco , Conteduca Vincenza , Santini Daniele , De Giorgi Ugo
Published: August 2023

Up to 80% of castration-resistant prostate cancer (CRPC) patients develop bone metastases during the natural history of disease and about 25% harbor mutations in DNA damage repair (DDR) genes. This retrospective observational study evaluated the prevalence of DDR alterations in CRPC patients and their effect on the clinical outcomes associated with bone metastases. The mutational status of CRPC patients was analyzed per FoundationOne® analysis in tissue biopsy or, when it was not possible, in liquid biopsy performed at the onset of metastatic CRPC (mCRPC). The impact of DDR gene mutations on bone-related efficacy endpoints was evaluated at the time of mCRPC diagnoses. In total, 121 mCRPC patients with bone metastases were included: 38 patients had mutations in at least one DDR gene, the remaining 83 ones had a non-mutated DDR status. DDR mutated status was associated with bone metastases volume (p = 0.006), but did not affect SRE (skeletal-related events) incidence and time to SRE onset. Liquid and tissue biopsies were both available for 61 patients with no statistically significant difference in terms of incidence and type of molecular DDR alterations. Mutated DDR status was associated with higher bone metastasic volume, although a not detrimental effect on the other bone-related efficacy endpoints was observed.
Circulating miR-206 and miR-1246 as Markers in the Early Diagnosis of Lung Cancer in Patients with Chronic Obstructive Pulmonary Disease
Authors: Córdoba-Lanús Elizabeth , Domínguez de-Barros Angélica , Oliva Alexis , Mayato Delia , Gonzalvo Francisca , Remírez-Sanz Ana , Zulueta Javier J. , Celli Bartolomé , Casanova Ciro
Published: August 2023

Lung cancer (LC) is the most common cause of cancer death, with 75% of cases being diagnosed in late stages. This study aimed to determine potential miRNAs as biomarkers for the early detection of LC in chronic obstructive pulmonary disease (COPD) cases. Ninety-nine patients were included, with registered clinical and lung function parameters followed for 6 years. miRNAs were determined in 16 serum samples from COPD patients (four with LC and four controls) by next generation sequencing (NGS) at LC diagnosis and 3 years before. The validation by qPCR was performed in 33 COPD-LC patients and 66 controls at the two time points. Over 170 miRNAs (≥10 TPM) were identified; among these, miR-224-5p, miR-206, miR-194-5p, and miR-1246 were significantly dysregulated (p < 0.001) in COPD-LC 3 years before LC diagnosis when compared to the controls. The validation showed that miR-1246 and miR-206 were differentially expressed in COPD patients who developed LC three years before (p = 0.035 and p = 0.028, respectively). The in silico enrichment analysis showed miR-1246 and miR-206 to be linked to gene mediators in various signaling pathways related to cancer. Our study demonstrated that miR-1246 and miR-206 have potential value as non-invasive biomarkers of early LC detection in COPD patients who could benefit from screening programs.
Comprehensive Analyses of Masticatory Function in Maxillectomy Patients with Functioning Removable Prostheses: A Retrospective Cross-Sectional Study
Authors: Kawasaki Masahiro , Ogino Yoichiro , Moroi Ryoji , Ayukawa Yasunori
Published: August 2023

The aim of this retrospective cross-sectional study was to comprehensively assess masticatory function in maxillectomy patients with functioning removable prostheses. Their general and oral profiles, the measurement values of their oral functions, including masticatory function, and the history of tumor therapy were extracted from medical charts. The correlations of masticatory function with numerical data and the effects of tumor therapy-related factors on masticatory function were evaluated. In addition, a stepwise conditional logistic regression analysis was performed to identify the potential predictive factors comprehensively. The data from 55 maxillectomy patients revealed that the median value of masticatory function (138.0 mg/dL) was higher than the threshold (100.0 mg/dL) based on the concept of oral hypofunction. Moderate correlations of masticatory function with the number of remaining teeth, the number of functioning occlusal supports, and maximum occlusal force were found, as well as a weak correlation with maximum tongue pressure. These variables also showed statistically significant coefficients (p < 0.01). No significant effect of each tumor therapy-related factor on masticatory function was detected. A logistic regression analysis identified the number of functioning occlusal supports as a significant predictive factor. These results implied the crucial interactions of masticatory function with various factors and the specificities of maxillectomy patients.
Cold Plasma Therapy in Chronic Wounds—A Multicenter, Randomized Controlled Clinical Trial (Plasma on Chronic Wounds for Epidermal Regeneration Study): Preliminary Results
Authors: Abu Rached Nessr , Kley Susanne , Storck Martin , Meyer Thomas , Stücker Markus
Published: August 2023

Chronic wounds (CWs) pose a significant health challenge in clinical practice. Standard wound therapy (SWT) is currently considered the gold standard. However, recent evidence suggests that cold plasma therapy (CPT) holds promise for improving CWs. In light of this, the POWER study was conducted as a multicenter, randomized clinical trial to investigate the effect of large-area plasma application compared with SWT in patients with chronic, non-healing arterial or venous wounds on the lower leg. To analyze the interim results, we employed a comprehensive range of statistical tests, including both parametric and non-parametric methods, as well as GLS model regression and an ordinal mixed model. Our findings clearly demonstrate that CPT therapy significantly accelerates wound closure compared with SWT. In fact, complete wound closure was exclusively observed in the CPT group during the intervention period. Additionally, the CPT group required significantly less antibiotic therapy (4%) compared with the SWT group (23%). Furthermore, CPT led to a significant reduction in wound pain and improved quality of life compared with SWT. In conclusion, the study highlights that the combination of CPT and SWT surpasses monotherapy with SWT alone.
Paraoxonase 1 Activity and Renal Replacement Therapy for Chronic Renal Failure: A Meta-Analysis
Authors: Watanabe Jun , Kotani Kazuhiko , Iwazu Yoshitaka , Gugliucci Alejandro
Published: August 2023

Chronic renal failure (CRF) is associated with the development of cardiovascular disease (CVD). Paraoxonase 1 (PON1), an antioxidant enzyme, shows cardioprotective properties and has been proposed as a therapeutic marker for CRF. A systematic analysis of the literature assessing the association between PON1 activity and renal replacement therapy (RRT) of CRF is currently lacking. Therefore, we set out to perform a meta-analysis of the available data on PON1 in RRT of CRF. We searched three electronic databases for studies on PON1 activity in CRF patients with RRT such as hemodialysis (HD), peritoneal dialysis (PD), or renal transplantation (RTx), published before June 2023. A random-effects and network meta-analysis were performed. A total of 53 studies were eligibly identified. Compared to CRF patients without RRT, RTx patients had higher paraoxonase activity (standard mean difference (SMD), 1.76, 95% confidence interval (CI), 0.76–2.75), followed by HD (SMD, 0.73; 95% CI, 0.02–1.45) and PD patients. Likewise, RTx patients had higher arylesterase activity (SMD, 1.84, 95% CI, 0.18–3.50), followed by HD and PD patients. Also, paraoxonase activity was increased after HD (SMD, 0.59, 95% CI, 0.16–1.03). In conclusion, the overall data demonstrated that PON1 activity is higher in CRF patients with RRT, particularly RTx, followed by that of HD and PD. Measuring PON1 activity can also be included to the paraclinical toolbox for the management of RRT, in addition to the understanding of CRF-related pathophysiology. Regarding the selection of RRT types and their potential to prevent CVD, more research is required.
From Nautical Waste to Additive Manufacturing: Sustainable Recycling of High-Density Polyethylene for 3D Printing Applications
Authors: Daniele Rigotti , Armoni Davide , Dul Sithiprumnea , Alessandro Pegoretti
Published: August 2023

High-density polyethylene (HDPE) is a highly versatile plastic utilized in various applicative fields such as packaging, agriculture, construction, and consumer goods. Unfortunately, the extensive use of polyethylene has resulted in a substantial accumulation of plastic waste, creating environmental and economic challenges. Consequently, the recycling of polyethylene has become a critical concern in recent times. This work focuses on the recycling of HDPE parts recovered from end-of-life boats into materials suitable for the marine environment with additive manufacturing technology via screw-assisted extrusion 3D printing. In particular, rigid materials are obtained by adding glass fibers to HDPE to mitigate the loss of mechanical performance upon recycling. Eventually, the properties obtained with two different production methods were compared, namely compression molding and screw-assisted extrusion 3D printing. Since the developed materials will be exposed to an aggressive environment, an extended thermos-mechanical characterization (including fatigue resistance) and investigation of the stability to UV exposure were performed.
Grain Refinement of Pure Magnesium for Microforming Application
Authors: Dwiyati Siska Titik , Kiswanto Gandjar , Supriadi Sugeng
Published: August 2023

Magnesium is a suitable candidate material for temporary implant applications, such as a miniplate, due to its biocompatibility, density, and elastic modulus comparable to that of human bone. The biodegradability property of magnesium can minimize the need for a second surgery after the healing process, thereby reducing costs and pain for patients. On the other hand, microforming is a promising technology for manufacturing miniplates with high production rates and good mechanical properties. However, the application of magnesium in microforming is limited and remains a challenge in resolving issues related to the size effect in microforming and the low formability of magnesium, especially at room temperature. Grain refinement and homogenization are alternative approaches to controlling the size effect in magnesium microforming and improving formability. As the grain refinement process influences the mechanical and corrosion behavior of magnesium, this research shows that the grain refinement process for pure magnesium improves the overall performance of the microforming process for implant applications.
Patterns of Public Spaces in Spanish Mediterranean Touristified Historic Centres Based on Their Activities: Case Study of Malaga
Authors: Conejo-Arrabal Francisco , Rosa-Jiménez Carlos , Nebot-Gómez de Salazar Nuria
Published: August 2023

Historic centres are undergoing a series of urban transformations as a consequence of the processes of touristification, and they are mainly located in pedestrianised public spaces. The consequences of the touristification of public space are manifested in its privatisation via the occupation of catering locals and changes to the uses of adjacent buildings. Recent literature has studied the touristification of the neighbourhood unit in an exhaustive way, but it has only studied specific variables of the public space unit. Therefore, an exhaustive study is needed to bring these variables together regarding the public space unit. This study proposes a methodology for categorising public space in terms of use, with the aim of identifying different patterns of activities with respect to touristification. To this end, a system of use indicators is defined according to the public space and adjacent buildings. This methodology has been tested in the Historic Centre of Malaga, analysing a sample of 54 public spaces and categorising them into five different patterns. This categorisation could facilitate the planning and regulation by local administrations of activities in the public space of the Historic Centre.
Repurposing Diltiazem for Its Neuroprotective Anti-Dementia Role against Intra-Cerebroventricular Streptozotocin-Induced Sporadic Alzheimer’s Disease-Type Rat Model
Authors: Alluri Ramesh , Kilari Eswar Kumar , Pasala Praveen Kumar , Kopalli Spandana Rajendra , Koppula Sushruta
Published: August 2023

Alzheimer’s disease (AD) is an age-related neuropsychiatric disorder and a common cause of progressive dementia. Diltiazem (DTZ), the non-dihydropyridine benzothiazepine class of calcium channel blocker (CCB), used clinically in angina and other cardiovascular disorders, has proven neurological benefits. In the present study, the neuroprotective anti-dementia effects of DTZ against intra-cerebroventricular-streptozotocin (ICV-STZ)-induced sporadic AD (SAD)-type rat model was investigated. ICV-STZ-induced cognitive impairments were measured via passive avoidance and Morris water maze tasks. Anti-oxidative enzyme status, pro-inflammatory markers, and amyloid-beta (Aβ) protein expression in rat brain tissues were measured using ELISA kits, Western blotting, and immunostaining techniques. The data revealed that ICV-STZ injection in rats significantly induced cognitive deficits and altered the levels of oxidative and pro-inflammatory markers (p < 0.05~p < 0.001). Treatment with DTZ (10 mg/kg, 20 mg/kg, and 40 mg/kg, p.o.) daily for twenty-one days, 1 h before a single ICV-STZ (3 mg/kg) injection, significantly improved cognitive impairments and ameliorated the ICV-STZ-induced altered nitrite, pro-inflammatory cytokines (TNF-α, and IL-1β) and anti-oxidative enzyme levels (superoxide dismutase, lipid peroxidation, and glutathione). Further, DTZ restored the increased Aβ protein expression in ICV-STZ-induced brain tissue. Considering the results obtained, DTZ might have a potential therapeutic role in treating and managing AD and related dementia pathologies due to its anti-dementia activity in SAD-type conditions in rats induced by ICV-STZ.
Research Progress in High-Performance Magnesium Alloy and Its Applications
Authors: Wu Di , Li Jinguo
Published: August 2023

Magnesium is abundant in the Earth’s crust and seawater [...]
Effect of the Load Application Angle on the Compressive Behavior of Al Honeycomb under Combined Normal–Shear Stress
Authors: Arquilla Giulia , Ceci Alessandra , Costanza Girolamo , Tata Maria Elisa
Published: August 2023

A comparison of the compressive behavior of Al honeycomb under pure normal stress and combined normal–shear stress was analyzed in this work. The typical working stress of honeycomb is a compressive load along the direction parallel to the axis of the cells. However, the component can also undergo shear stresses during operation, which can cause premature failure. This work analyzes the mechanical behavior in compression by normal stress (0°) and in conditions of combined normal–shear stress (at 15° and 25°) using a special pair of wedges. The samples were obtained from a 3000 series Al alloy sandwich panel and tested according to the ASTM C365/C365M-22 standard. The different deformation modes of the cells in the combined compression were examined for three angles (0, 15°, and 25°). A theoretical model of combined compression was used to derive the normal and tangential components starting from the total stress–strain curves. A compression curve analysis was conducted at different angles θ, allowing for considerations regarding changes in strength, absorbed energy, and deformations. Overall, as the load application angle increased, both the shear resistance of the honeycomb and its tangential displacement up to densification increased, which is the opposite of what occurs in normal behavior. The cell rotation angle was calculated as the load angle varied. The rotation angle of the cell increased with the displacement of the crosshead and the application angle of the force.
Identifying the Regions of a Space with the Self-Parameterized Recursively Assessed Decomposition Algorithm (SPRADA)
Authors: Molinié Dylan , Madani Kurosh , Amarger Véronique , Chebira Abdennasser
Published: August 2023

This paper introduces a non-parametric methodology based on classical unsupervised clustering techniques to automatically identify the main regions of a space, without requiring the objective number of clusters, so as to identify the major regular states of unknown industrial systems. Indeed, useful knowledge on real industrial processes entails the identification of their regular states, and their historically encountered anomalies. Since both should form compact and salient groups of data, unsupervised clustering generally performs this task fairly accurately; however, this often requires the number of clusters upstream, knowledge which is rarely available. As such, the proposed algorithm operates a first partitioning of the space, then it estimates the integrity of the clusters, and splits them again and again until every cluster obtains an acceptable integrity; finally, a step of merging based on the clusters’ empirical distributions is performed to refine the partitioning. Applied to real industrial data obtained in the scope of a European project, this methodology proved able to automatically identify the main regular states of the system. Results show the robustness of the proposed approach in the fully-automatic and non-parametric identification of the main regions of a space, knowledge which is useful to industrial anomaly detection and behavioral modeling.
Appropriateness of Dyslipidemia Management Strategies in Post-Acute Coronary Syndrome: A 2023 Update
Authors: Lucà Fabiana , Oliva Fabrizio , Rao Carmelo Massimiliano , Abrignani Maurizio Giuseppe , Amico Antonio Francesco , Di Fusco Stefania Angela , Caretta Giorgio , Di Matteo Irene , Di Nora Concetta , Pilleri Anna , Ceravolo Roberto , Rossini Roberta , Riccio Carmine , Grimaldi Massimo , Colivicchi Furio , Gulizia Michele Massimo
Published: August 2023

It has been consistently demonstrated that circulating lipids and particularly low-density lipoprotein cholesterol (LDL-C) play a significant role in the development of coronary artery disease (CAD). Several trials have been focused on the reduction of LDL-C values in order to interfere with atherothrombotic progression. Importantly, for patients who experience acute coronary syndrome (ACS), there is a 20% likelihood of cardiovascular (CV) event recurrence within the two years following the index event. Moreover, the mortality within five years remains considerable, ranging between 19 and 22%. According to the latest guidelines, one of the main goals to achieve in ACS is an early improvement of the lipid profile. The evidence-based lipid pharmacological strategy after ACS has recently been enhanced. Although novel lipid-lowering drugs have different targets, the result is always the overexpression of LDL receptors (LDL-R), increased uptake of LDL-C, and lower LDL-C plasmatic levels. Statins, ezetimibe, and PCSK9 inhibitors have been shown to be safe and effective in the post-ACS setting, providing a consistent decrease in ischemic event recurrence. However, these drugs remain largely underprescribed, and the consistent discrepancy between real-world data and guideline recommendations in terms of achieved LDL-C levels represents a leading issue in secondary prevention. Although the cost-effectiveness of these new therapeutic advancements has been clearly demonstrated, many concerns about the cost of some newer agents continue to limit their use, affecting the outcome of patients who experienced ACS. In spite of the fact that according to the current recommendations, a stepwise lipid-lowering approach should be adopted, several more recent data suggest a "strike early and strike strong" strategy, based on the immediate use of statins and, eventually, a dual lipid-lowering therapy, reducing as much as possible the changes in lipid-lowering drugs after ACS. This review aims to discuss the possible lipid-lowering strategies in post-ACS and to identify those patients who might benefit most from more powerful treatments and up-to-date management.
Silicon Carbide-Based DNA Sensing Technologies
Authors: Mamun Abdulla Al , McGarrity Mason , Kim Jong-Hoon , Zhao Feng
Published: August 2023

DNA sensing is critical in various applications such as the early diagnosis of diseases and the investigation of forensic evidence, food processing, agriculture, environmental protection, etc. As a wide-bandgap semiconductor with excellent chemical, physical, electrical, and biocompatible properties, silicon carbide (SiC) is a promising material for DNA sensors. In recent years, a variety of SiC-based DNA-sensing technologies have been reported, such as nanoparticles and quantum dots, nanowires, nanopillars, and nanowire-based field-effect-transistors, etc. This article aims to provide a review of SiC-based DNA sensing technologies, their functions, and testing results.
Magmatic Evolution and Rare Metal Mineralization in Mount El-Sibai Peralkaline Granites, Central Eastern Desert, Egypt: Insights from Whole-Rock Geochemistry and Mineral Chemistry Data
Authors: Sami Mabrouk , Osman Hassan , Ahmed Awaad F. , Zaky Khairy S. , Abart Rainer , Sanislav Ioan V. , Abdelrahman Kamal , Fnais Mohammed S. , Xiao Wenzhou , Abbas Hassan
Published: August 2023

The Ediacaran peralkaline granites, which were emplaced during the post-collisional tectonic extensional stage, have a limited occurrence in the northern tip of the Nubian Shield. In this contribution, we present new mineralogical and geochemical data of Mount El-Sibai granites from the Central Eastern Desert of Egypt. The aim is to discuss their crystallization condition, tectonic setting, and petrogenesis as well as the magmatic evolution of their associated mineralization. Mount El-Sibai consists of alkali-feldspar granites (AFGs) as a main rock unit with scattered and small occurrences of alkali-amphibole granites (AAGs) at the periphery. The AAG contain columbite, nioboaeschynite, zircon and thorite as important rare metal-bearing minerals. Geochemically, both of AFG and AAG exhibit a highly evolved nature with a typical peralkaline composition (A/CNK = 0.82–0.97) and formed in within-plate anorogenic setting associated with crustal extension and/or rifting. They are enriched in some LILEs (Rb, K, and Th) and HFSEs (Ta, Pb, Zr, and Y), but strongly depleted in Ba, Sr, P and Ti with pronounced negative Eu anomalies (Eu/Eu* = 0.07–0.34), consistent with an A-type granite geochemical signature. The calculated TZrn (774–878 °C) temperatures indicate that the magma was significantly hot, promoting the saturation of zircon. The texture and chemistry of minerals suggest that they were crystallized directly from a granitic magma and were later subject to late- to post-magmatic fluids. Both granitic types were most likely generated through partial melting of a juvenile crustal source followed by magmatic fractionation. The lithospheric delamination is the main mechanism which causes uplifting of the asthenospheric melts and hence provides enough heat for crustal melting. The produced parent magma was subjected to prolonged fractional crystallization to produce the different types of Mount El-Sibai granites at different shallow crustal levels. During magma fractionation, the post-magmatic fluids (especially fluorine) contribute significantly to the formation of rare metal mineralization within Mount El-Sibai granites.
From K6[Re6−xMoxS8(CN)5] Solid Solution to Individual Cluster Complexes: Separation and Investigation of [Re4Mo2S8(CN)6]n− and [Re3Mo3S8(CN)6]n− Heterometallic Clusters
Authors: Lappi Tatiana I. , Gayfulin Yakov M. , Renaud Adèle , Prestipino Carmelo , Lemoine Pierric , Yanshole Vadim V. , Muravieva Viktoria K. , Cordier Stéphane , Naumov Nikolai G.
Published: August 2023

A series of new cluster compounds with {Re4Mo2S8} and {Re3Mo3S8} cores has been obtained and investigated. The clusters with different Re/Mo ratios were isolated as individual compounds, which made it possible to study their spectroscopic and electrochemical properties. The geometry of the new clusters was studied using a combination of X-ray diffraction analysis, XAS and quantum chemical DFT calculations. It was shown that the properties of the new clusters, such as the number and position of electrochemical transitions, electronic structure and change in geometry with a change in charge, are similar to the properties of clusters based on the {Re4Mo2Se8} and {Re3Mo3Se8} cores described earlier.
Photochromic Azobenzene Inverse Opal Film toward Dynamic Anti-Fake Pattern
Authors: Liu Junchao , Han Zhitong , Wu Pingping , Shang Yuanyuan , Chen Jiansheng , Jia Pan
Published: August 2023

Azobenzene mesogens have garnered considerable research attention in the realm of photo-responsive materials due to their reversible trans–cis isomerization. In this paper, we demonstrate an azobenzene inverse opal film synthesized via photo-polymerization from a SiO2 opal template. The proposed design exhibits intriguing optical properties, including dynamic fluorescent features, distinct fluorescent enhancement, and an anti-fake micropattern with a switchable structure color. This work holds significant importance for advancing the development of novel optical devices.
The Diversity of Gut Microbiota at Weaning Is Altered in Prolactin Receptor-Null Mice
Authors: Luzardo-Ocampo Ivan , Ocampo-Ruiz Ana , Dena-Beltrán José , Martínez de la Escalera Gonzalo , Clapp Carmen , Macotela Yazmín
Published: August 2023

Maternal milk supports offspring development by providing microbiota, macronutrients, micronutrients, immune factors, and hormones. The hormone prolactin (PRL) is an important milk component with protective effects against metabolic diseases. Because maternal milk regulates microbiota composition and adequate microbiota protect against the development of metabolic diseases, we aimed to investigate whether PRL/PRL receptor signaling regulates gut microbiota composition in newborn mice at weaning. 16SrRNA sequencing of feces and bioinformatics analysis was performed to evaluate gut microbiota in PRL receptor-null mice (Prlr-KO) at weaning (postnatal day 21). The normalized colon and cecal weights were higher and lower, respectively, in the Prlr-KO mice relative to the wild-type mice (Prlr-WT). Relative abundances (Simpson Evenness Index), phylogenetic diversity, and bacterial concentrations were lower in the Prlr-KO mice. Eleven bacteria species out of 470 differed between the Prlr-KO and Prlr-WT mice, with two genera (Anaerotruncus and Lachnospiraceae) related to metabolic disease development being the most common in the Prlr-KO mice. A higher metabolism of terpenoids and polyketides was predicted in the Prlr-KO mice compared to the Prlr-WT mice, and these metabolites had antimicrobial properties and were present in microbe-associated pathogenicity. We concluded that the absence of the PRL receptor altered gut microbiota, resulting in lower abundance and richness, which could contribute to metabolic disease development.
Clinical Characteristics of 6102 Asymptomatic and Mild Cases for Patients with COVID-19 in Indonesia
Authors: Triyono Erwin Astha , Wahyuhadi Joni , Waloejo Christijogo Soemartono , Perdana Dimas Aji , Nabilah  , Dewanti Sisilia , Hidayat Amal Arifi , Lusida Michael Austin Pradipta , Sarasati Fani , Dharma Ngurah Arie Kapindra , Al Razzak Muhammad Ikhtiar Zaki , Wiranegara Tanri Hadinata , Ali Nurarifah Destianizar
Published: August 2023

Background: The COVID-19 pandemic has led to a rise in confirmed cases, making epidemiological studies crucial for identifying the source of transmission and developing effective treatment methods. We conducted a study on the clinical characteristics of patients with asymptomatic and mild symptoms of COVID-19 at a rescue hospital in Indonesia. Methods: This is an epidemiological study involving 6102 patients who were admitted to the Indrapura forefront hospital in Surabaya from May 2020 to February 2021. We described demographic data, clinical signs and symptoms, laboratory data, therapy, and clinical outcomes. Results: A total of 6102 patients were involved in this study, with 3664 (60.04%) being male and 2438 (39.95%) being female. The age range of 21–30 years was the most prevalent, accounting for 31.1% (1898 patients). The population had 1476 patients (24.2%) with comorbid conditions. The most prevalent comorbidity observed among these patients was hypertension, affecting 1015 individuals (16.6%). Out of the total 6006 patients observed, 40.7% (n = 2486) were asymptomatic, 54.6% (n = 3329) had mild symptoms, and 3.1% (n = 191) had moderate symptoms. All patients were administered supportive therapy without the use of antiviral medication. Out of the 6102 patients included in the study, 5923 patients (97.1%) achieved a cure, 36 patients (0.6%) are currently undergoing treatment, 142 patients (2.3%) were referred for desaturation indications (SpO2 < 94%), and one patient died due to a suspected cardiovascular event. Out of the total number of patients, 74.5% (4529 patients) had an average length of stay (LOS) of less than 10 days, while 25.6% (1563 patients) had an average length of stay of more than 10 days. Conclusion: The clinical presentation of asymptomatic and mild COVID-19 patients at a rescue hospital varies significantly based on the age and sex of patients. Cough and hyposmia are commonly observed symptoms. Supportive therapy is effective, and strict implementation of social distancing is crucial in preventing the spread of this disease from individuals who are asymptomatic or have mild symptoms.
Covalent Conjugates of Allylbenzenes and Terpenoids as Antibiotics Enhancers with the Function of Prolonged Action
Authors: Zlotnikov Igor D. , Davydova Maria P. , Danilov Milan R. , Krylov Sergey S. , Belogurova Natalya G. , Kudryashova Elena V.
Published: August 2023

The drug resistance of pathogenic bacteria is often due efflux pumps—specific proteins that remove foreign compounds from bacterial cells. To overcome drug resistance, adjuvants are often used that can inhibit efflux pumps or other systems that ensure the resistance of bacteria to the action of antibiotics. We assumed that a new level of effectiveness with the use of an antibiotic + an adjuvant pair could be achieved by their joint delivery into the pathogen. To test this hypothesis, we constructed a series of molecular carriers based on poly-(olygo-, dendry)mers based on cyclodextrin-grafted PEI or mannan, as well as glycol chitosan, covalently bound to antibiotic, adjuvant, and the oligosaccharide ligand to the macrophage mannose receptor (CD206), which we studied earlier and showed high efficiency and selectivity of delivery of a therapeutic “cargo” to macrophages. Moxifloxacin was used as an antibiotic, and terpenoid and allylbenzene compounds were used as adjuvants, for which we previously discovered the ability to inhibit bacterial efflux pumps. We show that: (a) the resulting structures were stable in vitro for a long time (up to 10 days); (b) they were adsorbed on bacterial cells, providing a local increase in the concentration of the antibiotic and adjuvant in pathogen cells; (c) they were internalized by bacterial cells, ensuring the accumulation of both antibiotic and adjuvant inside bacterial cells; (d) the adjuvant, after entering the bacterial cell, provided inhibition of the efflux pumps; (e) due to this action of the adjuvant, combined with the targeted delivery by the carrier, the antibiotic’s half-life in rats increased by more than 2 times, the effective concentration of the drug in the blood plasma (AUC) increased up to 8–10 times; (f) a significant increase in the effectiveness of the antibacterial action against Gram+ and Gram- cells was achieved (up to 3 times). Potentially, such an approach would significantly increase the effectiveness of therapies for a number of infectious and other diseases, reduce the dosage of antibiotics, shorten the duration of treatment, and reduce the risk of developing bacterial resistance. Moreover, the use of a polymer carrier with covalently bound organic molecules of different structures will avoid problems linked to different (suboptimal) solubility and bio-distribution of the administered molecules, which would be almost inevitable when using the same compounds separately. It would be very difficult to find antibiotic/adjuvant pairs that simultaneously achieve optimal concentrations in the same target cells. In our case, terpenoids and alkylbenzenes used as adjuvants are practically insoluble as individual compounds, and their unacceptable pharmacological properties would not allow them to be used as efflux pump inhibitors.
Stroke-Induced Central Pain: Overview of the Mechanisms, Management, and Emerging Targets of Central Post-Stroke Pain
Authors: Mohanan Anugeetha Thacheril , Nithya Sermugapandian , Nomier Yousra , Hassan Dalin A. , Jali Abdulmajeed M. , Qadri Marwa , Machanchery Shamna
Published: August 2023

The incidence of stroke plays the foremost role in the genesis of central neuropathic pain. Central post-stroke pain (CPSP) is a central pain arising from a vascular lesion in the central nervous system that elicits somatosensory deficits, often contralateral to stroke lesions. It is expressed as continuous or intermittent pain accompanied by sensory abnormalities like dysesthesia and allodynia. CPSP remains de-emphasized due to the variation in onset and diversity in symptoms, besides the difficulty of distinguishing it from other post-stroke pains, often referred to as a diagnosis of exclusion. Spinothalamic dysfunction, disinhibition of the medial thalamus, and neuronal hyperexcitability combined with deafferentation in thalamocortical regions are the mechanisms underlying central pain, which play a significant role in the pathogenesis of CPSP. The treatment regimen for CPSP seems to be perplexed in nature; however, based on available studies, amitriptyline and lamotrigine are denoted as first-line medications and non-pharmacological choices may be accounted for cases intractable to pharmacotherapy. This review attempts to provide an overview of the mechanisms, existing management approaches, and emerging targets of CPSP. A profound understanding of CPSP aids in optimizing the quality of life among stroke sufferers and facilitates further research to develop newer therapeutic agents for managing CPSP.
Depressive and Other Adverse CNS Effects of Fluoroquinolones
Authors: Wierzbiński Piotr , Hubska Joanna , Henzler Michał , Kucharski Bartłomiej , Bieś Rafał , Krzystanek Marek
Published: August 2023

Fluoroquinolones (FQs) are widely used drugs around the world. This is a result of their broad spectrum of antibacterial activity, high bioavailability, and known efficacy. Since they appeared on the market, their prescribing frequency has gradually increased. In 2011, FQs became the third most prescribed class of antibiotics in the US. Widespread use of these drugs resulted in an increasing number of reported side effects. In 2016, the FDA warned about significant side effects, including mental disorders in the form of anxiety, psychotic symptoms, insomnia, and depression. Psychiatric adverse reactions to FQs occur with a frequency of 1 to 4.4% and the mechanism of their formation is not entirely clear. It is believed that the antagonistic effect of FQs on the GABA receptor or interaction with the main receptor for the glutamatergic system—NMDA—is responsible for this. The paper is a structured review of 68 selected publications and the latest summary of CNS adverse effects that occur during FQ use. Prescribers should be aware of the risk factors for FQ toxicity, including elderly patients with underlying medical conditions or receiving concomitant medication; however, these adverse events may also occur in other groups of patients.
Correction: Ehlenfeldt et al. Asymmetric Reciprocal Crossing Behavior of an Andean Blueberry (V. meridionale) × Lingonberry (V. vitis-idaea) Hybrid. Plants 2022, 11, 3152
Authors: Ehlenfeldt Mark K. , Ogden Elizabeth , Rowland Lisa J.
Published: August 2023

In the original publication [...]