bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrative analysis of the 3D genome structure reveals that CTCF maintains the properties of mouse female germline stem cells
Authors: Tian Geng G. , Zhao Xinyan , Hou Changliang , Xie Wenhai , Li Xiaoyong , Wang Yinjuan , Wang Lijuan , Li Hua , Zhao Xiaodong , Li Jing , Wu Ji
Published: January 2022

The three-dimensional configuration of the genome ensures cell type-specific gene expression profiles by placing genes and regulatory elements in close spatial proximity. Here, we used in situ high-throughput chromosome conformation (in situ Hi-C), RNA sequencing (RNA-seq) and chromatin immunoprecipitation sequencing (ChIP-seq) to characterize the high-order chromatin structure signature of female germline stem cells (FGSCs) and identify its regulating key factor based on the data-driven of multiple omics data. By comparison with pluripotent stem cells (PSCs), adult stem cells (ASCs), and somatic cells at three major levels of chromatin architecture, A/B compartments, topologically associating domains, and chromatin loops, the chromatin architecture of FGSCs was most similar to that of other ASCs and largely different from that of PSCs and somatic cells. After integrative analysis of the three-dimensional chromatin structure, active compartment-associating loops (aCALs) were identified as a signature of high-order chromatin organization in FGSCs, which revealed that CCCTC-binding factor was a major factor to maintain the properties of FGSCs through regulation of aCALs. We found FGSCs belong to ASCs at chromatin structure level and characterized aCALs as the high-order chromatin structure signature of FGSCs. Furthermore, CTCF was identified to play a key role in regulating aCALS to maintain the biological functions of FGSCs. These data provide a valuable resource for future studies of the features of chromatin organization in mammalian stem cells and further understanding of the fundamental characteristics of FGSCs.
Parallel Packing Squares into a Triangle
Authors: Januszewski Janusz , Liu Xi , Su Zhanjun , Zielonka Łukasz
Published: January 2022

Assume that$$T_h$$This a triangle with the interior angles at the base of the measure not greater than$$90^0$$900, with the base length 1 and the heighth. LetSbe a square with a side parallel to the base of$$T_h$$Thand let$$\{S_n\}$${Sn}be a collection of the homothetic copies ofS. A tight upper bound of the sum of the areas of squares from$$\{S_n\}$${Sn}that can be parallel packed into a triangle$$T_h$$This determined.
Molecular super-gluing: a straightforward tool for antibody labelling and its application to mycotoxin biosensing
Authors: Pradanas-González Fernando , Glahn-Martínez Bettina , Benito-Peña Elena , Arola Henri O. , Nevanen Tarja K. , Moreno-Bondi María C.
Published: January 2022

Mycotoxins are low molecular weight toxic compounds, which can cause severe health problems in animals and humans. Immunoassays allow rapid, simple and cost-effective screening of mycotoxins. Sandwich assays with a direct readout provide great improvement in terms of selectivity and sensitivity, compared to the widely used competitive assay formats, for the analysis of low molecular weight molecules. In this work, we report a non-competitive fluorescence anti-immune complex (IC) immunoassay, based on the specific recognition of HT-2 toxin with a pair of recombinant antibody fragments, namely antigen-binding fragment (Fab) (anti-HT-2 (10) Fab) and single-chain variable fragment (scFv) (anti-IC HT-2 (10) scFv). The SpyTag and SpyCatcher glue proteins were applied for the first time as a bioconjugation tool for the analysis of mycotoxins. To this aim, a SpyTag-mScarlet-I (fluorescent protein) and scFv-SpyCatcher fusion proteins were constructed, produced and fused in situ during the assay by spontaneous Tag-Catcher binding. The assay showed an excellent sensitivity with an EC50 of 4.8 ± 0.4 ng mL−1 and a dynamic range from 1.7 ± 0.3 to 13 ± 2 ng mL−1, an inter-day reproducibility of 8.5% and a high selectivity towards HT-2 toxin without cross-reactivity with other Fusarium toxins. The bioassay was applied to the analysis of the toxin in an oat reference material and in oat samples, with a LOD of 0.6 µg kg−1, and the results were validated by analysing a certificate reference material and by HPLC–MS/MS.

Graphical abstract
Anatomical variation of inner ear may be a predisposing factor for unilateral Ménière’s disease rather than for ipsilateral delayed endolymphatic hydrops
Authors: Lei Ping , Leng Yangming , Li Jing , Zhou Renhong , Liu Bo
Published: January 2022

Objective

Radiological anatomical variations, measured by magnetic resonance imaging (MRI), were evaluated in patients with ipsilateral delayed endolymphatic hydrops (DEH) and unilateral Ménière’s disease (MD). The role of anatomical variations in different subtypes of hydropic ear disease was investigated.

Methods

Twenty-eight patients with ipsilateral DEH, 76 patients with unilateral MD, and 59 control subjects were enrolled. The radiological indices included the distance between the vertical part of the posterior semicircular canal and the posterior fossa (MRI-PP distance) and the visibility of vestibular aqueduct (MRI-VA). These variations among patients with DEH, MD, and control subjects were compared. The correlation between radiological anatomical variations and clinical features or audio-vestibular findings was also examined.

Results

(1) MRI-PP distance in the affected side of unilateral MD was shorter than that in ipsilateral DEH (Z =  − 2.481, p = 0.013) and control subjects (Z =  − 2.983, p = 0.003), while the difference of MRI-PP distance between the affected side of ipsilateral DEH and control subjects was not statistically significant (Z =  − 0.859, p = 0.391). (2) There was no significant interaural difference of MRI-PP distance in patients with unilateral MD (Z =  − 0.041, p = 0.968) and ipsilateral DEH (t =  − 0.107, p = 0.915) respectively. (3) No significant interaural difference of MRI-VA visibility was observed in patients with unilateral MD (χ2 = 0.742, p = 0.389) and ipsilateral DEH (χ2 = 0.327, p = 0.567) respectively. (4) No correlation was found between these anatomical variables and clinical features or audio-vestibular findings in patients with unilateral MD and ipsilateral DEH respectively (p > 0.05).

Conclusions

Anatomical variations of inner ear may be a predisposing factor in the pathogenesis of unilateral MD rather than ipsilateral DEH.

Key Points

• Patients with ipsilateral delayed endolymphatic hydrops showed normal distance between the vertical part of the posterior semicircular canal and the posterior fossa.

• Compared to patients with ipsilateral delayed endolymphatic hydrops and control subjects, patients with unilateral Ménière’s disease exhibited shorter distance between the vertical part of the posterior semicircular canal and the posterior fossa.

• Anatomical variations of inner ear may be a predisposing factor in the pathogenesis of unilateral Ménière’s disease rather than ipsilateral delayed endolymphatic hydrops.
Repeated intermittent administration of (R)-ketamine during juvenile and adolescent stages prevents schizophrenia-relevant phenotypes in adult offspring after maternal immune activation: a role of TrkB signaling
Authors: Tan Yunfei , Fujita Yuko , Pu Yaoyu , Chang Lijia , Qu Youge , Wang Xinming , Hashimoto Kenji
Published: January 2022

Maternal immune activation (MIA) plays a role in the etiology of schizophrenia. MIA by prenatal exposure of polyinosinic:polycytidylic acid [poly(I:C)] in rodents caused behavioral and neurobiological changes relevant to schizophrenia in adult offspring. We investigated whether the novel antidepressant (R)-ketamine could prevent the development of psychosis-like phenotypes in adult offspring after MIA. We examined the effects of (R)-ketamine (10 mg/kg/day, twice weekly for 4 weeks) during juvenile and adolescent stages (P28–P56) on the development of cognitive deficits, loss of parvalbumin (PV)-immunoreactivity in the medial prefrontal cortex (mPFC), and decreased dendritic spine density in the mPFC and hippocampus from adult offspring after prenatal poly(I:C) exposure. Furthermore, we examined the role of TrkB in the prophylactic effects of (R)-ketamine. Repeated intermittent administration of (R)-ketamine during juvenile and adolescent stages significantly blocked the development of cognitive deficits, reduced PV-immunoreactivity in the prelimbic (PrL) of mPFC, and decreased dendritic spine density in the PrL of mPFC, CA3 and dentate gyrus of the hippocampus from adult offspring after prenatal poly(I:C) exposure. Furthermore, pretreatment with ANA-12 (TrkB antagonist: twice weekly for 4 weeks) significantly blocked the beneficial effects of (R)-ketamine on cognitive deficits of adult offspring after prenatal poly(I:C) exposure. These data suggest that repeated intermittent administration of (R)-ketamine during juvenile and adolescent stages could prevent the development of psychosis in adult offspring after MIA. Therefore, (R)-ketamine would be a potential prophylactic drug for young subjects with high-risk for psychosis.
There is no correlation between a delayed gastric conduit emptying and the occurrence of an anastomotic leakage after Ivor-Lewis esophagectomy
Authors: Babic Benjamin , Schiffmann Lars Mortimer , Fuchs Hans Friedrich , Mueller Dolores Thea , Schmidt Thomas , Mallmann Christoph , Mielke Laura , Frebel Antonia , Schiller Petra , Bludau Marc , Chon Seung-Hun , Schroeder Wolfgang , Bruns Christiane Josephine
Published: January 2022

Introduction

Esophagectomy is the gold standard in the surgical therapy of esophageal cancer. It is either performed thoracoabdominal with a intrathoracic anastomosis or in proximal cancers with a three-incision esophagectomy and cervical reconstruction. Delayed gastric conduit emptying (DGCE) is the most common functional postoperative disorder after Ivor-Lewis esophagectomy (IL). Pneumonia is significantly more often in patients with DGCE. It remains unclear if DGCE anastomotic leakage (AL) is associated. Aim of our study is to analyze, if AL is more likely to happen in patients with a DGCE.

Patients and methods

816 patients were included. All patients have had an IL due to esophageal/esophagogastric-junction cancer between 2013 and 2018 in our center. Intrathoracic esophagogastric end-to-side anastomosis was performed with a circular stapling device. The collective has been divided in two groups depending on the occurrence of DGCE. The diagnosis DGCE was determined by clinical and radiologic criteria in accordance with current international expert consensus.

Results

27.7% of all patients suffered from DGCE postoperatively. Female patients had a significantly higher chance to suffer from DGCE than male patients (34.4% vs. 26.2% vs., p = 0.040). Pneumonia was more common in patients with DGCE (13.7% vs. 8.5%, p = 0.025), furthermore hospitalization was longer in DGCE patients (median 17 days vs. 14d, p < 0.001). There was no difference in the rate of type II anastomotic leakage, (5.8% in both groups DGCE). All patients with ECCG type II AL (n = 47; 5.8%) were treated successfully by endoluminal/endoscopic therapy. The subgroup analysis showed that ASA ≥ III (7.6% vs. 4.4%, p = 0.05) and the histology squamous cell carcinoma (9.8% vs. 4.7%, p = 0.01) were independent risk factors for the occurrence of an AL.

Conclusion

Our study confirms that DGCE after IL is a common finding in a standardized collective of patients in a high-volume center. This functional disorder is associated with a higher rate of pneumonia and a prolonged hospital stay. Still, there is no association between DGCE and the occurrence of an AL after esophagectomy. The hypothesis, that an DGCE results in a higher pressure on the anastomosis and therefore to an AL in consequence, can be refuted. DGCE is not a pathogenetic factor for an AL.
A two-level macroscale continuum description with embedded discontinuities for nonlinear analysis of brick/block masonry
Authors: Pantò B. , Macorini L. , Izzuddin B. A.
Published: January 2022

A great proportion of the existing architectural heritage, including historical and monumental constructions, is made of brick/block masonry. This material shows a strong anisotropic behaviour resulting from the specific arrangement of units and mortar joints, which renders the accurate simulation of the masonry response a complex task. In general, mesoscale modelling approaches provide realistic predictions due to the explicit representation of the masonry bond characteristics. However, these detailed models are very computationally demanding and mostly unsuitable for practical assessment of large structures. Macroscale models are more efficient, but they require complex calibration procedures to evaluate model material parameters. This paper presents an advanced continuum macroscale model based on a two-scale nonlinear description for masonry material which requires only simple calibration at structural scale. A continuum strain field is considered at the macroscale level, while a 3D distribution of embedded internal layers allows for the anisotropic mesoscale features at the local level. A damage-plasticity constitutive model is employed to mechanically characterise each internal layer using different material properties along the two main directions on the plane of the masonry panel and along its thickness. The accuracy of the proposed macroscale model is assessed considering the response of structural walls previously tested under in-plane and out-of-plane loading and modelled using the more refined mesoscale strategy. The results achieved confirm the significant potential and the ability of the proposed macroscale description for brick/block masonry to provide accurate and efficient response predictions under different monotonic and cyclic loading conditions.
Quaternion methods and models of regular celestial mechanics and astrodynamics
Authors: Chelnokov Y. N.
Published: January 2022

This paper is a review, which focuses on our work, while including an analysis of many works of other researchers in the field of quaternionic regularization. The regular quaternion models of celestial mechanics and astrodynamics in the Kustaanheimo-Stiefel (KS) variables and Euler (Rodrigues-Hamilton) parameters are analyzed. These models are derived by the quaternion methods of mechanics and are based on the differential equations of the perturbed spatial two-body problem and the perturbed spatial central motion of a point particle. This paper also covers some applications of these models. Stiefel and Scheifele are known to have doubted that quaternions and quaternion matrices can be used efficiently to regularize the equations of celestial mechanics. However, the author of this paper and other researchers refuted this point of view and showed that the quaternion approach actually leads to efficient solutions for regularizing the equations of celestial mechanics and astrodynamics.

This paper presents convenient geometric and kinematic interpretations of the KS transformation and the KS bilinear relation proposed by the present author. More general (compared with the KS equations) quaternion regular equations of the perturbed spatial two-body problem in the KS variables are presented. These equations are derived with the assumption that the KS bilinear relation was not satisfied. The main stages of the quaternion theory of regularizing the vector differential equation of the perturbed central motion of a point particle are presented, together with regular equations in the KS variables and Euler parameters, derived by the aforementioned theory. We also present the derivation of regular quaternion equations of the perturbed spatial two-body problem in the Levi-Civita variables and the Euler parameters, developed by the ideal rectangular Hansen coordinates and the orientation quaternion of the ideal coordinate frame.

This paper also gives new results using quaternionic methods in the perturbed spatial restricted three-body problem.
Risk Assessment—the Key to More Security? Factors, Tools, and Practices in Dealing with Extremist Individuals
Authors: Höffler Katrin , Meyer Miriam , Möller Veronika
Published: January 2022

In recent years, risk assessments for violent extremism have attracted great interest from both scholars and practitioners, and many assessment tools have been developed. After a critical review of this development, the paper examines differences and similarities between the indicators of various violent extremist risk assessment tools and checklists. Based on an interview study with 34 experts in the field of Counter Violent Extremism and 24 (formerly) radicalized persons from the right-wing radical and Salafi-Jihadist spectrum, risk factors were identified and the findings merged and compared with already existing risk assessment tools. The paper will present results especially regarding the risk signals’ occurrence and applicability in the German context. One key finding is that existing assessment tools insufficiently take into account personal contacts in the radicalization process. Thus, the paper—based on the results of a social network analysis—draws attention to the potential and importance of networks. The paper concludes by outlining the potential of risk assessment, suggesting improvements, and raising awareness of the limits and deficits of these tools. The paper thus scientifically addresses the challenge of more security through efficient risk assessment and management. It offers a list of radicalization process characteristics (ARISNA: Assessment of Radicalized Individuals including Social Network Analysis), which is designed to help users analyze the risk of radicalization based on concrete traits of a person and their environment.
Relative free-energy calculations for scaffold hopping-type transformations with an automated RE-EDS sampling procedure
Authors: Ries Benjamin , Normak Karl , Weiß R. Gregor , Rieder Salomé , Barros Emília P. , Champion Candide , König Gerhard , Riniker Sereina
Published: January 2022

The calculation of relative free-energy differences between different compounds plays an important role in drug design to identify potent binders for a given protein target. Most rigorous methods based on molecular dynamics simulations estimate the free-energy difference between pairs of ligands. Thus, the comparison of multiple ligands requires the construction of a “state graph”, in which the compounds are connected by alchemical transformations. The computational cost can be optimized by reducing the state graph to a minimal set of transformations. However, this may require individual adaptation of the sampling strategy if a transformation process does not converge in a given simulation time. In contrast, path-free methods like replica-exchange enveloping distribution sampling (RE-EDS) allow the sampling of multiple states within a single simulation without the pre-definition of alchemical transition paths. To optimize sampling and convergence, a set of RE-EDS parameters needs to be estimated in a pre-processing step. Here, we present an automated procedure for this step that determines all required parameters, improving the robustness and ease of use of the methodology. To illustrate the performance, the relative binding free energies are calculated for a series of checkpoint kinase 1 inhibitors containing challenging transformations in ring size, opening/closing, and extension, which reflect changes observed in scaffold hopping. The simulation of such transformations with RE-EDS can be conducted with conventional force fields and, in particular, without soft bond-stretching terms.
Binding free energies for the SAMPL8 CB8 “Drugs of Abuse” challenge from umbrella sampling combined with Hamiltonian replica exchange
Authors: Markthaler Daniel , Kraus Hamzeh , Hansen Niels
Published: January 2022

Umbrella sampling along a one-dimensional order parameter in combination with Hamiltonian replica exchange was employed to calculate the binding free energy of five guest molecules with known affinity to cucurbit[8]uril. A simple empirical approach correcting for the overestimation of the affinity by the GAFF force field was proposed and subsequently applied to the seven guest molecules of the “Drugs of Abuse” SAMPL8 challenge. Compared to the uncorrected binding free energies, the systematic error decreased but quantitative agreement with experiment was only reached for a few compounds. From a retrospective analysis a weak point of the correction term was identified.
Intrahousehold Bargaining Power in Spain: An Empirical Test of the Collective Model
Authors: Molina José Alberto , Velilla Jorge , Ibarra Helena
Published: January 2022

This paper analyzes the intrahousehold bargaining power of spouses in Spanish families, in a collective framework. We estimate household labor supply equations and, under certain testable restrictions, we obtain a theoretically derived sharing rule for household income, which characterizes intrahousehold bargaining power. Then, using unique data on decision-making in the household, we construct Pareto weights, and study the validity of the collective model by comparing the theoretical sharing rule and the constructed Pareto weight. The results reveal that both the observed Pareto weight and the theoretical sharing rule display qualitative similarities, thus providing direct empirical support to the collective model. Furthermore, the results suggest that Spanish wives behave more altruistically, while husbands behave more egoistically. This should be taken into account by policy makers and researchers when analyzing inequality in the household, and contemplating specific policies affecting the household.
Comparison of algorithms for error prediction in manufacturing with automl and a cost-based metric
Authors: Gerling Alexander , Ziekow Holger , Hess Andreas , Schreier Ulf , Seiffer Christian , Abdeslam Djaffar Ould
Published: January 2022

In order to manufacture products at low cost, machine learning (ML) is increasingly used in production, especially in high wage countries. Therefore, we introduce our PREFERML AutoML system, which is adapted to the production environment. The system is designed to predict production errors and to help identifying the root cause. It is particularly important to produce results for further investigations that can also be used by quality engineers. Quality engineers are not data science experts and are usually overwhelmed with the settings of an algorithm. Because of this, our system takes over this task and delivers a fully optimized ML model as a result. In this paper, we give a brief overview of what results can be achieved with a state-of-the-art classifier. Moreover, we present the results with optimized tree-based algorithms based on RandomSearchCV and HyperOpt hyperparameter tuning. The algorithms are optimized based on multiple metrics, which we will introduce in the following sections. Based on a cost-oriented metric we can show an improvement for companies to predict the outcome of later product tests. Further, we compare the results from the mentioned optimization approaches and evaluate the needed time for them.
Tunable magnetocaloric effect in amorphous Gd-Fe-Co-Al-Si alloys
Authors: Lindner Natalia , Śniadecki Zbigniew , Kołodziej Mieszko , Grenèche Jean-Marc , Marcin Jozef , Škorvánek Ivan , Idzikowski Bogdan
Published: January 2022

A magnetocaloric effect with wide tunability was observed in melt-spun amorphous Gd65Fe15-xCo5+xAl10Si5 (x = 0, 5, 10) alloys of different Fe/Co ratios. Their magnetic properties were compared with those of the previously investigated parent alloy Gd65Fe10Co10Al15. The glassy structure of the melt-spun samples was confirmed by X-ray diffraction (XRD) and 57Fe Mössbauer spectrometry. Their Curie temperatures (TC) were between 155 and 195 K and increased significantly with decreasing Co content. The highest value of the magnetic entropy change ΔSM = − 6.8 J/kg K was obtained for Gd65Fe5Co15Al10Si5, when the magnetic field was increased from 0 to 5 T. Refrigerant capacity (RC) takes values close to 700 J/kg for the whole series of the alloys. The occurrence of the second-order phase transition and the conformity of the magnetic behavior with the mean field model were concluded on the basis of the analysis of the universal curves and the values of the exponent n (ΔSM ∝ Hn).

Graphical abstract
In situ polymerization monitoring of a diacrylate in an electrically conducting mesoporous nanoparticle scaffold
Authors: Hoffmann Ron , Naatz Hendrik , Hartwig Andreas
Published: January 2022

The properties of nanoparticle–polymer composites strongly depend on the network structure of the polymer matrix. By introducing nanoparticles into a monomer (solution) and subsequently polymerizing it, the formation of the polymer phase influences the mechanical and physicochemical properties of the composite. In this study, semi-conducting indium tin oxide (ITO) nanoparticles were prepared to form a rigid nanoparticle scaffold in which 1,6-hexanediol diacrylate (HDDA), together with an initiator for photo-polymerization, was infiltrated and subsequently polymerized by UV light. During this process, the polymerization reaction was characterized using rapid scan Kubelka–Munk FT-IR spectroscopy and compared to bulk HDDA. The conductivity change of the ITO nanoparticles was monitored and correlated with the polymerization process. It was revealed that the reaction rates of the radical initiation and chain propagation are reduced when cured inside the voids of the nanoparticle scaffold. The degree of conversion is lower for HDDA infiltrated into the mesoporous ITO nanoparticle scaffold compared to purely bulk-polymerized HDDA.

Graphical abstract
PEEK filament characteristics before and after extrusion within fused filament fabrication process
Authors: Comelli Cleiton André , Davies Richard , van der Pol HenkJan , Ghita Oana
Published: January 2022

The heating and extrusion process in fused filament fabrication (FFF) is significantly shorter than the conventional extrusion process where longer heating times and significant pressure are applied. For this reason, it is important to understand whether the crystal history of the feedstock is fully erased through the FFF process and whether the FFF process can be tailored further by engineering the crystallization of the feedstock filaments. In this context, a methodology for evaluating the influence of morphology and mechanical properties on different feedstock and extruded filaments is proposed. Filaments with three different PEEK 450G crystalline structures (standard crystallinity, drawn filament and amorphous filament) were selected and evaluated, before and after free extrusion. The resulting morphology, crystallinity and mechanical properties of the extruded filaments were compared against the feedstock properties. X-ray diffraction (XRD), transmission electron microscopy (TEM), differential and fast scanning calorimetry (DSC/FDSC) and tensile test were the techniques used to evaluate the materials. The results showed clear differences in the properties of the feedstock materials, while the analysis of the extruded filaments points to a homogenization of the resulting material producing mostly similar mechanical properties. However, the use of the drawn filament highlighted a statistically significant improvement in crystallinity and mechanical performance, especially in strain values. This conclusion suggests the innovative possibility of improving the quality of manufactured parts by tailoring the microstructure of the feedstock material used in the FFF process.

Graphical abstract
A viewpoint on material and design considerations for oesophageal stents with extended lifetime
Authors: Jackson Caitlin E. , Johnson Liam S. J. , Williams Dominic A. , Laasch Hans-Ulrich , Edwards Derek W. , Harvey Alison G.
Published: January 2022

Oesophageal stents are meshed tubular implants designed to maintain patency of the oesophageal lumen and attenuate the symptoms of oesophageal cancer. Oesophageal cancers account for one in twenty cancer diagnoses and can lead to dysphasia, malnutrition and the diminishment of patient quality of life (QOL). Self-expanding oesophageal stents are the most common approach to attenuate these symptoms. Recent advances in oncological therapy have enabled patient survival beyond the lifetime of current devices. This introduces new complications for palliation, driving the need for innovation in stent design. This review identifies the factors responsible for stent failure. It explores the challenges of enhancing the longevity of stent therapies and outlines solutions to improving clinical outcomes. Discussions focus on the role of stent materials, construction methods, and coatings upon device performance. We found three key stent enhancement strategies currently used; material surface treatments, anti-migratory modifications, and biodegradable skeletons. Furthermore, radioactive and drug eluting stent designs were identified as emerging novel treatments. In conclusion, the review offers an overview of remaining key challenges in oesophageal stent design and potential solutions. It is clear that further research is needed to improve the clinical outcome of stents and patient QOL.
Conductivity, microstructure and mechanical properties of tape-cast LATP with LiF and SiO2 additives
Authors: Gross Jürgen Peter , Malzbender Jürgen , Dashjav Enkhtsetseg , Tietz Frank , Schwaiger Ruth
Published: January 2022

LATP sheets with LiF and SiO2 addition prepared by tape cast as electrolytes for solid-state batteries were characterized regarding conductivity, microstructure and mechanical properties aiming toward an optimized composition. The use of additives permitted a reduction of the sintering temperature. Rietveld analyses of the samples with additives revealed a phase mixture of NaSICON modifications crystallizing with rhombohedral and orthorhombic symmetry as a superstructure with space group Pbca. It seems that LiF acts as a sintering additive but also as a mineralizer for the superstructure of LATP. As general trend, higher LiF to SiO2 ratios led to lower porosities and higher values of elastic modulus and hardness determined by indentation testing, but the presence of the orthorhombic LATP leads to a decrease in the ionic conductivity. Micro-pillar testing was used to assess the crack growth behavior revealing weak grain boundaries.
A study of tensile and bending properties of 3D-printed biocompatible materials used in dental appliances
Authors: García Reyes Marcos , Bataller Torras Alex , Cabrera Carrillo Juan A. , Velasco García Juan M. , Castillo Aguilar Juan J.
Published: January 2022

In the last years, a large number of new biocompatible materials for 3D printers have emerged. Due to their recent appearance and rapid growth, there is little information about their mechanical properties. The design and manufacturing of oral appliances made with 3D printing technologies require knowledge of the mechanical properties of the biocompatible material used to achieve optimal performance for each application. This paper focuses on analysing the mechanical behaviour of a wide range of biocompatible materials using different additive manufacturing technologies. To this end, tensile and bending tests on different types of recent biocompatible materials used with 3D printers were conducted to evaluate the influence of the material, 3D printing technology, and printing orientation on the fragile/ductile behaviour of the manufactured devices. A test bench was used to perform tensile tests according to ASTM D638 and bending tests according to ISO 178. The specimens were manufactured with nine different materials and five manufacturing technologies. Furthermore, specimens were created with different printing technologies, biocompatible materials, and printing orientations. The maximum allowable stress, rupture stress, flexural modulus, and deformation in each of the tested specimens were recorded. Results suggest that specimens manufactured with Stereolithography (SLA) and milling (polymethyl methacrylate PMMA) achieved high maximum allowable and rupture stress values. It was also observed that Polyjet printing and Selective Laser Sintering technologies led to load–displacement curves with low maximum stress and high deformation values. Specimens manufactured with Digital Light Processing technology showed intermediate and homogeneous performance. Finally, it was observed that the printing direction significantly influences the mechanical properties of the manufactured specimens in some cases.
Global optimization for the multilevel European gas market system with nonlinear flow models on trees
Authors: Schewe Lars , Schmidt Martin , Thürauf Johannes
Published: January 2022

The European gas market is implemented as an entry-exit system, which aims to decouple transport and trading of gas. It has been modeled in the literature as a multilevel problem, which contains a nonlinear flow model of gas physics. Besides the multilevel structure and the nonlinear flow model, the computation of so-called technical capacities is another major challenge. These lead to nonlinear adjustable robust constraints that are computationally intractable in general. We provide techniques to equivalently reformulate these nonlinear adjustable constraints as finitely many convex constraints including integer variables in the case that the underlying network is tree-shaped. We further derive additional combinatorial constraints that significantly speed up the solution process. Using our results, we can recast the multilevel model as a single-level nonconvex mixed-integer nonlinear problem, which we then solve on a real-world network, namely the Greek gas network, to global optimality. Overall, this is the first time that the considered multilevel entry-exit system can be solved for a real-world sized network and a nonlinear flow model.
The robust bilevel continuous knapsack problem with uncertain coefficients in the follower’s objective
Authors: Buchheim Christoph , Henke Dorothee
Published: January 2022

We consider a bilevel continuous knapsack problem where the leader controls the capacity of the knapsack and the follower chooses an optimal packing according to his own profits, which may differ from those of the leader. To this bilevel problem, we add uncertainty in a natural way, assuming that the leader does not have full knowledge about the follower’s problem. More precisely, adopting the robust optimization approach and assuming that the follower’s profits belong to a given uncertainty set, our aim is to compute a solution that optimizes the worst-case follower’s reaction from the leader’s perspective. By investigating the complexity of this problem with respect to different types of uncertainty sets, we make first steps towards better understanding the combination of bilevel optimization and robust combinatorial optimization. We show that the problem can be solved in polynomial time for both discrete and interval uncertainty, but that the same problem becomes NP-hard when each coefficient can independently assume only a finite number of values. In particular, this demonstrates that replacing uncertainty sets by their convex hulls may change the problem significantly, in contrast to the situation in classical single-level robust optimization. For general polytopal uncertainty, the problem again turns out to be NP-hard, and the same is true for ellipsoidal uncertainty even in the uncorrelated case. All presented hardness results already apply to the evaluation of the leader’s objective function.
Optimistic NAUTILUS navigator for multiobjective optimization with costly function evaluations
Authors: Saini Bhupinder Singh , Emmerich Michael , Mazumdar Atanu , Afsar Bekir , Shavazipour Babooshka , Miettinen Kaisa
Published: January 2022

We introduce novel concepts to solve multiobjective optimization problems involving (computationally) expensive function evaluations and propose a new interactive method called O-NAUTILUS. It combines ideas of trade-off free search and navigation (where a decision maker sees changes in objective function values in real time) and extends the NAUTILUS Navigator method to surrogate-assisted optimization. Importantly, it utilizes uncertainty quantification from surrogate models like Kriging or properties like Lipschitz continuity to approximate a so-called optimistic Pareto optimal set. This enables the decision maker to search in unexplored parts of the Pareto optimal set and requires a small amount of expensive function evaluations. We share the implementation of O-NAUTILUS as open source code. Thanks to its graphical user interface, a decision maker can see in real time how the preferences provided affect the direction of the search. We demonstrate the potential and benefits of O-NAUTILUS with a problem related to the design of vehicles.
Exploratory Considerations in Chemistry Education—Didactic Modelling for Complexity in Students’ Discussions
Authors: Dudas Cecilia , Rundgren Carl-Johan , Lundegård Iann
Published: January 2022

Research has shown the importance of dealing with real-life issues and of enabling student encounters with complexity in chemistry education in order to increase student participation. Therefore, this study aims to analyse how complexity evolves in students’ discussions and how this complexity relates to aspects of tentativeness in chemistry. In the study, we analyse how a previously developed didactic model can be refined from the students’ considerations evolving from the present context. The study was conducted as an in situ study in one upper-secondary school. Students’ discussions were recorded on video. The recordings were transcribed and analysed using deliberative educational questions. Two different kinds of considerations emerged in the students’ discussions: factual and exploratory considerations. While factual considerations are an important element of chemistry education, students also need to encounter exploratory considerations. The study proposes a didactic model useful for teachers in didactic analysis and design of activities aiming to support students to unfold complexity through exploratory considerations. One implication is to base activities on real-life issues in order to invite the unpredictability needed for experiencing complexity and the exploratory nature of chemistry. These issues enable students to experience aspects of tentativeness in chemistry and thereby increase their understanding of NOS and chemistry as a knowledge building practice. Furthermore, this might also increase student participation in chemistry education.
Purinergic receptors mediate endothelial dysfunction and participate in atherosclerosis
Authors: Wu Xian-Ming , Zhang Ning , Li Jiang-Shan , Yang Zhi-Hong , Huang Xiao-Lou , Yang Xiao-Fang
Published: January 2022

Atherosclerosis is the main pathological basis of cardiovascular disease and involves damage to vascular endothelial cells (ECs) that results in endothelial dysfunction (ED). The vascular endothelium is the key to maintaining blood vessel health and homeostasis. ED is a complex pathological process involving inflammation, shear stress, vascular tone, adhesion of leukocytes to ECs, and platelet aggregation. The activation of P2X4, P2X7, and P2Y2 receptors regulates vascular tone in response to shear stress, while activation of the A2A, P2X4, P2X7, P2Y1, P2Y2, P2Y6, and P2Y12 receptors promotes the secretion of inflammatory cytokines. Finally, P2X1, P2Y1, and P2Y12 receptor activation regulates platelet activity. These purinergic receptors mediate ED and participate in atherosclerosis. In short, P2X4, P2X7, P2Y1, and P2Y12 receptors are potential therapeutic targets for atherosclerosis.
The Comparison of the Dietary Intake Loss Between Elderly and Non-Elderly Patients After Gastrectomy for Gastric Cancer
Authors: Nakazono Masato , Aoyama Toru , Komori Keisuke , Watanabe Hayato , Kano Kazuki , Nagasawa Shinsuke , Segami Kenki , Tamagawa Hiroshi , Yukawa Norio , Rino Yasushi , Ogata Takashi , Oshima Takashi
Published: January 2022

Background/Aim

The present study quantified the changes in the dietary and nutritional intake after gastrectomy between elderly and non-elderly patients.

Patients and Methods

This prospective observational study enrolled patients who underwent curative gastrectomy for gastric cancer.

Results

Twenty-three patients ≥ 75 years old were classified into the elderly group, and 127 patients < 75 years old were classified into the non-elderly group. The respective median % dietary intake losses at 1 and 3 months postoperatively were −12.4% and −5.3% in the elderly group and −8.3% and −2.8% in the non-elderly group (p = 0.075 and 0.080). On comparing the intake loss of three major nutrients, the respective median % lipid intake losses at 1 and 3 months postoperatively were −13.5% and −5.8% in the elderly group and −7.3% and 0% in the non-elderly group (p = 0.029 and 0.045).

Conclusion

Our results suggested that elderly patients experienced more serious lipid intake loss after gastrectomy than non-elderly patients.
Should Violence Against Robots be Banned?
Authors: Mamak Kamil
Published: January 2022

This paper addresses the following question: “Should violence against robots be banned?” Such a question is usually associated with a query concerning the moral status of robots. If an entity has moral status, then concomitant responsibilities toward it arise. Despite the possibility of a positive answer to the title question on the grounds of the moral status of robots, legal changes are unlikely to occur in the short term. However, if the matter regardspublicviolence rather than mere violence, the issue of the moral status of robots may be avoided, and legal changes could be made in the short term. Prohibition of public violence against robots focuses on public morality rather than on the moral status of robots. The wrongness of such acts is not connected with the intrinsic characteristics of robots but with their performance in public. This form of prohibition would be coherent with the existing legal system, which eliminates certain behaviors in public places through prohibitions against acts such as swearing, going naked, and drinking alcohol.
Toxicants, entanglement, and mitigation in New England’s emerging circular economy for food waste
Authors: Isenhour Cindy , Haedicke Michael , Berry Brieanne , MacRae Jean , Blackmer Travis , Horton Skyler
Published: January 2022

Drawing on research with food waste recycling facilities in New England, this paper explores a fundamental tension between the eco-modernist logics of the circular economy and the reality of contemporary waste streams. Composting and digestion are promoted as key solutions to food waste, due to their ability to return nutrients to agricultural soils. However, our work suggests that food waste processors increasingly find themselves responsible for policing boundaries between distinct “material” and “biological” systems as imagined by the architects of the circular economy—boundaries penetrable by toxicants. This responsibility creates significant problems for processors due to the regulatory, educational, and structural barriers documented in this research. This paper contributes to scholarship which suggests the need to rethink the modernist logics of the circular economy and to recognize the realities of entangled material and biological systems. More specifically, we argue that if circularity is the goal, policy needs to recognize the barriers food waste processors face and concentrate circularity efforts further upstream to ensure fair, just, and safe circular food systems.
Overview on the digitized production of welded steel structures
Authors: Skriko Tuomas , Ahola Antti , Björk Timo
Published: January 2022

This paper presents a concept and practical topics involved in digitized production. The term “production” denotes the design, fabrication, and service life of a product, which in this case elaborates on welded steel structures. This includes the required information for guiding all the process stages of the chosen material back to its re-melting, following the material flow in a fully digitized form. This concept enables an increase in production quality at a higher level while minimizing the risk of human errors. Automation of the short-run production of steel structures for demanding applications is also a key goal, together with securing a cost-efficient process. Typically, such structures are fabricated from high- or ultra-high-strength steels. Though challenging, reaching these aims seems to be realistic by applying advanced fatigue design methods, using high-quality robotic welding and receiving information about the real loading of the structure.
Sleep Apnea Syndrome (SAS) Clinical Practice Guidelines 2020
Authors: Akashiba Tsuneto , Inoue Yuichi , Uchimura Naohisa , Ohi Motoharu , Kasai Takatoshi , Kawana Fusae , Sakurai Shigeru , Takegami Misa , Tachikawa Ryo , Tanigawa Takeshi , Chiba Shintaro , Chin Kazuo , Tsuiki Satoru , Tonogi Morio , Nakamura Hiroshi , Nakayama Takeo , Narui Koji , Yagi Tomoko , Yamauchi Motoo , Yamashiro Yoshihiro , Yoshida Masahiro , Oga Toru , Tomita Yasuhiro , Hamada Satoshi , Murase Kimihiko , Mori Hiroyuki , Wada Hiroo , Uchiyama Makoto , Ogawa Hiromasa , Sato Kazumichi , Nakata Seiichi , Mishima Kazuo , Momomura Shin-Ichi
Published: January 2022

The prevalence of sleep-disordered breathing (SDB) is reportedly very high. Among SDBs, the incidence of obstructive sleep apnea (OSA) is higher than previously believed, with patients having moderate-to-severe OSA accounting for approximately 20% of adult males and 10% of postmenopausal women not only in Western countries but also in Eastern countries, including Japan. Since 1998, when health insurance coverage became available, the number of patients using continuous positive airway pressure (CPAP) therapy for sleep apnea has increased sharply, with the number of patients about to exceed 500,000 in Japan. Although the “Guidelines for Diagnosis and Treatment of Sleep Apnea Syndrome (SAS) in Adults” was published in 2005, a new guideline was prepared to indicate the standard medical care based on the latest trends, as supervised by and in cooperation with the Japanese Respiratory Society and the “Survey and Research on Refractory Respiratory Diseases and Pulmonary Hypertension” Group, of Ministry of Health, Labor and Welfare and other related academic societies, including the Japanese Society of Sleep Research, in addition to referring to the previous guidelines. Since sleep apnea is an interdisciplinary field covering many areas, this guideline was prepared including 36 clinical questions (CQs). In the English version, therapies and managements for SAS, which were written from CQ16 to 36, were shown. The Japanese version was published in July 2020 and permitted as well as published as one of the Medical Information Network Distribution Service (Minds) clinical practice guidelines in Japan in July 2021.
The Future of IVF: The New Normal in Human Reproduction
Authors: Kushnir Vitaly A. , Smith Gary D. , Adashi Eli Y.
Published: January 2022

Increased demand for in vitro fertilization (IVF) due to socio-demographic trends, and supply facilitated by new technologies, converged to transform the way a substantial proportion of humans reproduce. The purpose of this article is to describe the societal and demographic trends driving increased worldwide demand for IVF, as well as to provide an overview of emerging technologies that promise to greatly expand IVF utilization and lower its cost.