bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of sugar-sweetened soda on plasma saturated and monounsaturated fatty acids in individuals with obesity: A randomized study
Authors: Bajahzer Mohammed Fahad , Bruun Jens Meldgaard , Rosqvist Fredrik , Marklund Matti , Richelsen Bjørn , Risérus Ulf
Published: August 2022

Background

High carbohydrate, i.e., sugars, intake potentially drives the liver into a lipogenic state leading to elevated plasma fatty acids. Excessive intake of saturated fat and sugar-sweetened soda induces liver fat accumulation, but studying the effect of high intake from sugar-sweetened soda on the de novo lipogenesis (DNL) fatty acids in long-term randomized trials is lacking.

Objective

To study the effect of consuming 1 L/day of sugar-sweetened soda, semi-skimmed milk (milk), aspartame-sweetened soda or water over 24 weeks on DNL-derived fatty acids (i.e., palmitate (primary outcome) and other saturated and monounsaturated fatty acids), and markers of stearoyl-CoA desaturase activity (SCD1) in plasma phospholipids (PL), cholesteryl esters (CE), and triglycerides (TG).

Design and methods

A randomized parallel study was conducted simultaneously at Aarhus University Hospital and Copenhagen University, Denmark, including (n = 41) individuals aged 20–50 years, with BMI of 26–40 kg/m2, and without diabetes. The groups consisted of 9 individuals in the sugar-sweetened soda, 10 in the milk, 11 in the aspartame-sweetened soda, and 11 in the water. The change at 24 weeks was assessed and compared across the groups using ANCOVA and mixed-effects models. Correlations of fatty acid changes with liver fat accumulation (magnetic resonance imaging) were explored.

Results

After 24 weeks, the groups differed in palmitate proportions in PL, oleate in CE and PL, and palmitoleate and SCD1 in all fractions (p < 0.05). Compared with water, the relative proportion of palmitate in PL increased by approximately 1% during both sugar-sweetened soda (p = 0.011) and milk (p = 0.006), whereas oleate and palmitoleate increased only during sugar-sweetened soda (CE 2.77%, p < 0.001; PL 1.51%, p = 0.002 and CE 1.46%, PL 0.24%, TG 1.31%, all p < 0.001, respectively). Liver fat accumulation correlated consistently with changes in palmitoleate, whereas correlations with palmitate and oleate were inconsistent across lipid fractions.

Conclusions

Although both sugar-sweetened soda and milk increased palmitate in PL, only excess intake of sugar-sweetened soda increased palmitoleate in all lipid fractions and correlated with liver fat. In contrast, isocaloric milk intake did not increase plasma monounsaturated fatty acids.

Clinical trial registration

[https://clinicaltrials.gov/ct2/show/NCT00777647], identifier [NCT00777647].
A Pilot Pre and Post 4 Week Intervention Evaluating the Effect of a Proprietary, Powdered, Plant Based Food on Micronutrient Status, Dietary Intake, and Markers of Health in a Healthy Adult Population
Authors: Wilcox Matthew D. , Chater Peter I. , Stanforth Kyle J. , Williams Rebecca , Brownlee Iain A. , Pearson Jeffrey P.
Published: August 2022

Background

A “balanced, adequate, and varied diet” is recommended as the basis of nutritionally sound diet by the World Health Organisation and national public health agencies. Huel is a proprietary, on-the-go, powdered, plant based food, providing all 26 essential vitamins and minerals, protein, essential fats, carbohydrate, fibre, and phytonutrients.

Objectives

Assessing the effect of solely consuming Huel on micronutrient status, dietary intake and markers of health was achieved through a 4-week intervention of solely Huel powder.

Methods

Habitual energy intake was assessed through a one-week lead in period with healthy adult participants (aged 18 or over) logging their food intake, after which only Huel was consumed for 4 weeks. Blood samples and body composition was assessed before and after the lead in week as well the end of the intervention. Thirty participants were recruited with 20 (11 females, median age 31, range 22–44) completing the study, 19 sets of blood samples were collected. 22 blood markers were analysed along with weight, BMI, waist circumference, visceral adipose tissue (VAT), and body composition. All blood micronutrients, except for Thyroid Stimulating Hormone and choline were sent to Royal Victoria Infirmary NHS, Newcastle Laboratory (Newcastle upon Tyne, United Kingdom) for analysis.

Results

Fourteen of the parameters significantly changed over the course of the study with circulating haemoglobin, iron, vitamins B12 and D as well as selenium significantly increasing (p < 0.05). HbA1c, total and non-HDL cholesterol, vitamins A and E, potassium, BMI, VAT, and waist circumference all significantly decreased (p < 0.05) post intervention.

Conclusion

Although energy intake decreased during the intervention period, the adherence to recommended micronutrient intake, as quantified by the dietary Total Adherence Score, significantly increased which tallies with the preservation or improvement of micronutrient status. This study potentially demonstrates that consuming only Huel for 4 weeks does not negatively affect micronutrient status.
Physiological and biochemical evaluation of high anthocyanin pigmented tea (Camellia sinensis L. O. Kuntze) germplasm for purple tea production
Authors: Patel Pradeep Kumar , Siddiqui Shahida Anusha , Kuča Kamil , Sabhapondit Santanu , Sarma Rupak , Gogoi Boby , Singh Shobhit Kumar , Bordoloi Ranjeet Kumar , Saikia Jayanta Kumar , Gogoi Romen Chandra , Bhardwaj Kanchan , Yang Jie , Tao Yang , Manickam Sivakumar , Das Buddhadeb
Published: August 2022

Finding promising purple tea germplasm that would target new tea products for diversification and value addition boost the tea industry’s economic growth. Accordingly, 10 tea germplasm viz. TRA St. 817, TRA St. 293, TRA St. 400, TRA 177/3, TRA 376/2, TRA 376/3, TRA 427/7, TRA P7, TRA P8, and TV1 were evaluated in terms of gas exchange parameters, multiplication performance, and biochemical markers such as chlorophyll, carotenoids, and anthocyanin content, which are related to the purple tea quality. The investigated gas exchange and biochemical parameters revealed significant differences. Germplasm TRA St.817 was physiologically more efficient (24.7 μmol m–2 s–1), followed by TRA St. 293, exhibiting the highest net photosynthesis, water use efficiency (19.02 μmol mmol–1), carboxylation efficiency (0.73), chlorophyll fluorescence or photochemical efficiency of PSII (0.754) and mesophyll efficiency (ci/gs ratio: 2.54). Net photosynthesis was positively correlated with water use efficiency, carboxylation efficiency, mesophyll efficiency, and photochemical efficiency of PSII (r = 0.965**, 0.937**, 0.857**, 0.867**; P = 0.05), respectively, but negatively correlated with the transpiration ratio (r = −0.878**; P = 0.05) based on Pearson correlation analysis. The total anthocyanin content (4764.19 μg.g–1 fresh leaf weight) and carotenoid content (3.825 mg.g–1 fresh leaf weight) were highest in the TRA St.817 germplasm, followed by germplasm TRA St. 293 (2926.18 μg.g–1 FW). In contrast, total chlorophyll content was significantly low (1.779 mg.g–1 fresh weight), which is very suitable for manufacturing purple tea. The highest carotenoid concentration in TRA St. 817 was 3.825 mg.g–1 FW, followed by TRA P8 (3.475 mg.g–1 FW), favoring the formation of more volatile flavor constituents. The promising germplasm, TRA St 817, has a multiplication success rate of 91.4% through cleft grafting. The outcome reveals that TRA St.817 is a promising germplasm that can be used to make speciality teas, i.e., purple tea.
Identification and validation of the role of c-Myc in head and neck squamous cell carcinoma
Authors: Zhao Sufeng , An Li , Yang Xudong , Wei Zheng , Zhang He , Wang Yufeng
Published: August 2022

Background

Many studies have shown that c-Myc plays a critical role in tumorigenesis. However, the molecular role of c-Myc in head and neck squamous cell carcinoma (HNSC) remains unclear.

Methods

Several biological databases, including UALCAN, TIMER2.0, TCGAportal, GEPIA, KM plotter, OncoLnc, LinkedOmics, GSCA, and TCIA, were used to analyze the molecular role of c-Myc in HNSC. The expression levels of c-Myc were validated by real-time PCR (RT–PCR) and Western blot in CAL-27 cells.

Results

The expression of c-Myc mRNA were significantly increased in HPV-negative HNSC tissues. The expression of c-Myc gene level was correlated with TP53 mutation status. HNSC also showed hypomethylated c-Myc compared with normal tissues. c-Myc was identified as an ominous prognostic factor for HNSC patients and correlated with immune infiltrating levels. Moreover, high c-Myc expression was associated with decreased expression of a series of immune checkpoints, resulting in a dampened immune response. c-Myc potentially mediated IL-17 signaling pathway and Th1 and Th2 cell differentiation. Inhibition of c-Myc expression increased apoptosis of CAL-27 cells.

Conclusions

These findings suggest a new mechanism of c-Myc in the prognosis of HNSC, implying the potential of c-Myc as a therapeutic target for HNSC patients.
Relationship of dose to vascular target volumes and local failure in pancreatic cancer patients undergoing neoadjuvant chemoradiation
Authors: Nelson Bailey , Barrord Michelle , Wang Kyle , Wages Nolan A. , Sudhoff Mickaela , Kharofa Jordan
Published: August 2022

Objective

The objectives of this study were to evaluate whether dose to the vasculature is associated with local control after surgery in patients with borderline resectable (BLR) and resectable pancreatic cancer (PCA) receiving neoadjuvant radiation therapy (RT) and to identify a dose threshold for clinical use.

Methods

Patients with BLR and resectable PCA treated with neoadjuvant RT were retrospectively reviewed. During this period, the institutional paradigm shifted from standard fractionation to hypofractionation/stereotactic body radiation therapy (SBRT). A vasculature clinical target volume (Vasc CTV) was contoured for each patient and defined as a 5-mm margin around the superior mesenteric artery (SMA) from its origin to the pancreatic head, the celiac artery from its origin to the level of the trifurcation and any involved vein. The Vasc CTV D95 was normalized to a 2-Gy equivalent dose to determine the optimal dose associated with optimal local failure-free survival (LFFS).

Results

Forty-seven patients were included in the analysis. A Vasc CTV D95 of 32.7 Gy was the optimal cutoff for LFFS. Patients with Vasc CTV D95 Equivalent dose in 2 Gy per fraction (EQD2) >32.7 Gy had significantly longer LFFS compared to patients with Vasc CTV D95 EQD2 ≤32.7 Gy at 12 months (91% vs. 51%, respectively) and 24 months (86% vs. 12%, respectively). The median disease-free survival (DFS) for patients with EQD2 >32.7 Gy was 30.4 months compared to 14.0 months in patients with EQD2 ≤32.7 Gy (p = 0.01). There was no significant difference in overall survival (OS) between the two groups.

Conclusions

During neoadjuvant treatment, dose to the Vasc CTV is associated with durability of local control (LC) after resection and should be intentionally included in the treatment volume with an EQD2 goal of 31–33 Gy.
Efficacy and safety of taxanes combined with chemotherapy drugs in advanced triple negative breast cancer: A meta-analysis of 26 randomized controlled trials
Authors: Huang Qionglian , Mei Zubing , Han Xianghui
Published: August 2022

Background

Researchers have demonstrated that the combined use of taxanes and chemotherapy drugs, especially paclitaxel-based treatment, appeared to clinically benefit on advanced triple negative breast cancer (TNBC). This meta-analysis aims to obtain the existent evidence on efficacy and safety for taxanes-based combination therapy to treat advanced TNBC.

Methods

From 1991 to June 2022, seven databases (PubMed, Web of Science, Cochrane Library, Embase VIP, Wanfang, and CNKI databases) were comprehensively searched with no restricted language and region. The included randomized controlled trials (RCTs) compared taxanes-based combination therapy versus taxanes or other chemotherapy drugs. Statistical analysis was conducted using random-effect model, and the quality of RCTs was assessed using the tool of Cochrane Collaboration risk of bias.

Results

Twenty-six RCTs with a total of 8,236 advanced TNBC patients were included. Compared with taxanes monotherapy, taxanes-based combination therapy significantly prolonged progression-free survival (HR=0.79, 95%CI=0.74–0.83, I2= 0.0%, p=0.000) and overall survival (HR=0.88, 95%CI=0.82–0.94, I2= 9.3%, p=0.000) and increased the risk of vomiting (RR=1.26, 95%CI=1.07–1.48) and diarrhea (RR=1.82, 95%CI=1.22–2.70, I2= 90.3%, p=0.003). No statistical differences were observed in complete response rate (CRR), objective response rate (ORR), disease control rate (DCR), and progressive disease (PD) indexes (CRR: RR=1.38, 95%CI=0.96–1.99; ORR: RR=1.20, 95%CI=0.73–1.98; DCR: RR=1.09, 95%CI=1.00–1.19; PD: RR=0.70, 95%CI=0.47–1.04). Compared with other chemotherapy drugs, taxanes plus other chemotherapy drugs significantly reduced the incidence of vomiting (RR=0.60, 95%CI=0.44–0.84, I2= 12.3%, p=0.002) and neutropenia (RR=0.58, 95%CI=0.35–0.96, I2= 73.0%, p=0.036) during the treatment period.

Conclusions

Taxanes-based combination therapy is evidently effective and well-tolerated in advanced TNBC, indicating that it might be a recommended option for treating advanced TNBC patients to some extent.

Systematic Review Registration

https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42022337802.
ROHHAD syndrome without rapid-onset obesity: A diagnosis challenge
Authors: Desse Blandine , Tran Antoine , Butori Mathilde , Marchal Sarah , Afanetti Michael , Barthélemy Sébastien , Bérard Etienne , Baechler Elisabeth , Debelleix Stéphane , Lampin Marie-Emilie , Macey Julie , Massenavette Bruno , Harvengt Julie , Trang Ha , Giovannini-Chami Lisa
Published: August 2022

Background

ROHHAD syndrome (Rapid-onset Obesity with Hypothalamic dysfunction, Hypoventilation and Autonomic Dysregulation) is rare. Rapid-onset morbid obesity is usually the first recognizable sign of this syndrome, however a subset of patients develop ROHHAD syndrome without obesity. The prevalence of this entity is currently unknown. Alteration of respiratory control as well as dysautonomic disorders often have a fatal outcome, thus early recognition of this syndrome is essential.

Material and methods

A retrospective, observational, multicenter study including all cases of ROHHAD without rapid-onset obesity diagnosed in France from 2000 to 2020.

Results

Four patients were identified. Median age at diagnosis was 8 years 10 months. Median body mass index was 17.4 kg/m2. Signs of autonomic dysfunction presented first, followed by hypothalamic disorders. All four patients had sleep apnea syndrome. Hypoventilation led to the diagnosis. Three of the four children received ventilatory support, all four received hormone replacement therapy, and two received psychotropic treatment. One child in our cohort died at 2 years 10 months old. For the three surviving patients, median duration of follow-up was 7.4 years.

Conclusion

ROHHAD syndrome without rapid-onset obesity is a particular entity, appearing later than ROHHAD with obesity. This entity should be considered in the presence of dysautonomia disorders without brain damage. Likewise, the occurrence of a hypothalamic syndrome with no identified etiology requires a sleep study to search for apnea and hypoventilation. The identification of ROHHAD syndrome without rapid-onset obesity is a clinical challenge, with major implications for patient prognosis.
Tibetan medicine salidroside improves host anti-mycobacterial response by boosting inflammatory cytokine production in zebrafish
Authors: He Shumei , Fan Hongyan , Sun Bin , Yang Meipan , Liu Hongxu , Yang Jianwei , Liu Jianxin , Luo Sizhu , Chen Zihan , Zhou Jing , Xia Lu , Zhang Shulin , Yan Bo
Published: August 2022

The treatment for tuberculosis (TB), especially multidrug-resistant TB (MDR-TB), has a prolonged cycle which can last up to a year. This is partially due to the lack of effective therapies. The development of novel anti-TB drugs from the perspective of host immune regulation can provide an important supplement for conventional treatment strategies. Salidroside (SAL), a bioactive component from the Tibetan medicine Rhodiola rosea, has been used in the treatment of TB, although its mechanism remains unclear. Here, the bacteriostatic effect of SAL in vivo was first demonstrated using a zebrafish–M. marinum infection model. To further investigate the underlying mechanism, we then examined the impact of SAL on immune cell recruitment during wound and infection. Increased macrophage and neutrophil infiltrations were found both in the vicinity of the wound and infection sites after SAL treatment compared with control, which might be due to the elevated chemokine expression levels after SAL treatment. SAL treatment alone was also demonstrated to improve the survival of infected zebrafish larvae, an effect that was amplified when combining SAL treatment with isoniazid or rifampicin. Interestingly, the reduced bacterial burden and improved survival rate under SAL treatment were compromised in tnfα-deficient embryos which suggests a requirement of Tnfα signaling on the anti-mycobacterial effects of SAL. In summary, this study provides not only the cellular and molecular mechanisms for the host anti-mycobacterial effects of the Tibetan medicine SAL but also proof of concept that combined application of SAL with traditional first-line anti-TB drugs could be a novel strategy to improve treatment efficacy.
Herbal medicine for amyotrophic lateral sclerosis: A systematic review and meta-analysis
Authors: Song Yuebo , Jia Qiuyang , Guan Xiaorui , Kazuo Sugimoto , Liu Jia , Duan Weisong , Feng Luda , Zhang Chi , Gao Ying
Published: August 2022

Background: The effect of herbal medicine (HM) on amyotrophic lateral sclerosis (ALS) is controversial. Clinical trials investigating HMs continue; however, the use of HM is still questioned. We aimed to systematically review the literature pertaining to the effects and safety of HM in ALS.

Methods: Randomised controlled trials (RCTs) that investigated the efficacy of HMs in ALS patients compared to any types of controls were identified. Nine databases and six registers were searched from their inception dates to 25 March 2022. Per the PRISMA guidelines, trials were identified and extracted. The risk of bias was evaluated using the Cochrane’s tool. Certainty of evidence was assessed as per the GRADE criteria. Forest plots were constructed to assess the effect size and corresponding 95% CIs using fixed-effect models, and random-effect models were employed when required. The primary outcome was the activity limitation measured by validated tools, such as the revised ALS Functional Rating Scale.

Results: Twenty studies (N = 1,218) were eligible. Of these, only five studies were double-blinded, and two were placebo-controlled. Fourteen HMs (fifty-one single botanicals) were involved; Astragalus mongholicus Bunge, Atractylodes macrocephala Koidz., and Glycyrrhiza glabra L. were commonly used in nine, eight, and six trials, respectively. For delaying activity limitation, Jiweiling injection (MD, 2.84; 95% CI, 1.21 to 4.46; p = 0.0006) and Shenmai injection (SMD, 1.07; 0.69 to 1.45; p < 0.00001) were significantly more efficacious than Riluzole, but the evidence was low quality. For ameliorating motor neuron loss, Jiweiling injection [right abductor pollicis brevis (APB): MD, 32.42; 7.91 to 56.93; p = 0.01 and left APB: MD, 34.44; 12.85 to 56.03; p = 0.002] was favoured, but the evidence was very low quality. Nine studies reported one hundred and twenty-three adverse events, twenty-six of which occurred in the treatment groups and ninety-seven in the control groups.

Conclusion: Very low to low quality of evidence suggests that HMs seem to produce superior treatment responses for ALS without increased risk of adverse events. Additional studies with homogeneous participants, reduced methodological issues, and more efficient outcome measures are required to provide confirmatory evidence.

Systematic Review Registration:https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42021277443.
Beneficial effect of Xuebijing against Pseudomonas aeruginosa infection in Caenorhabditis elegans
Authors: Zhang Le , Wang Yuxing , Cao Chang , Zhu Yike , Huang Wei , Yang Yi , Qiu Haibo , Liu Songqiao , Wang Dayong
Published: August 2022

In the clinical intensive care units (ICU), the traditional Chinese medicine (TCM) formulation of Xuebijing has been frequently used for treating sepsis. Nevertheless, the underlying pharmacological mechanisms of Xuebijing remain largely unclear. Caenorhabditis elegans is an important experimental host for bacterial infections. Using C. elegans as an animal model, we here examined the potential of Xuebijing treatment against bacterial infection and the underlying mechanisms. Xuebijing treatment could inhibit the reduction tendency of lifespan caused by Pseudomonas aeruginosa infection. For the cellular mechanisms of this antibacterial infection property, we found that Xuebijing treatment rescued C. elegans lifespan to be against P. aeruginosa infection by inhibiting Pseudomonas colonization in the intestinal lumen. Meanwhile, the increase in the expression of antimicrobial genes induced by Pseudomonas infection was also suppressed by Xuebijing treatment. Moreover, the beneficial effect of Xuebijing against Pseudomonas infection depended on insulin, p38 MAPK, Wnt, DBL-1/TGF-β, ELT-2, and programmed cell death (PCD)-related signals. Although Xuebijing did not show obvious antibacterial activity, Xuebijing (100%) treatment could inhibit the Pseudomonas biofilm formation and decrease the expression of virulence genes (lasA, lasB, rhlA, rhlC, phzA, phzM, phzH, and phzS) and quorum sensing (QS)-related genes (lasI, lasR, rhlI, rhlR, pqsA, and pqsR). Our results support the potential role of Xuebijing treatment against bacterial infection in hosts.
Poldip2 knockdown protects against lipopolysaccharide-induced acute lung injury via Nox4/Nrf2/NF-κB signaling pathway
Authors: Wang Yueguo , Wang Wenwen , Zhou Shusheng , Wang Yulan , Cudjoe Obed , Cha Yu , Wang Chunyan , Cao Xiaoguang , Liu Wei , Jin Kui
Published: August 2022

Polymerase δ-interacting protein 2 (Poldip2) has been reported to mediate acute lung injury (ALI); however, the underlying mechanism is not fully explored. Male C57BL/6 mice and A549 cells were used to establish the lipopolysaccharide (LPS)-induced ALI model, then the expression of Poldip2 and its effect on oxidative stress and the resulting inflammation were detected. Adeno-associated virus serotype 6 (AAV6) mediated Poldip2 knockdown was transfected into mice via intratracheal atomization. And A549 cells stimulated with LPS was used to further confirm our hypothesis in vitro. ML385, specifically inhibited the activation of the Nrf2 signaling pathway. Our data suggested that LPS stimulation remarkably increased protein levels of Nox4 and p-P65, activities of NADPH and MPO, and generation of ROS, TNF-α, and IL-1β while decreased protein levels of Nrf2 and HO-1 compared with those in NC shRNA + Saline group, which were obviously reversed by Poldip2 knockdown. Concomitantly, Poldip2 knockdown dramatically reduced contents of MDA and enhanced activities of SOD and GSH-Px compared to NC shRNA + LPS group. In vitro, we found that knockdown of Poldip2 significantly reversed LPS-induced increase protein levels of Nox4 and p-P65, activity of NADPH, and generation of ROS, TNF-α, and IL-1β, and decrease protein levels of Nrf2 and HO-1, ML385 pretreatment reversed the effects of Poldip2 knockdown mentioned above. Our study indicated that Poldip2 knockdown alleviates LPS-induced ALI via inhibiting Nox4/Nrf2/NF-κB signaling pathway.
An Adversarial Dynamic Game to Controlling Information Diffusion under Typical Strategies on Online Social Networks
Authors: Liu Yifan , Zeng Ruinan , Chen Lili , Wang Zhen , Hu Liqin
Published: August 2022

The diffusion of negative information, such as rumours, misinformation and computer viruses on Online Social Networks (OSNs), may lead to serious losses and consequences. And there are always some rational malicious spreaders, who strategically spread negative information. Therefore, how to control the information diffusion of the malicious spreader is a great challenge. In recent years, some studies have analyzed the controlling problem which belongs to the issue of influence blocking maximization (IBM) from the perspective of the large-scale strategy set on the game theory. However, the aforementioned methods cannot timely solve the controlling diffusion problem on high-speed OSNs. In this study, we achieve the purpose of effectively controlling diffusion on OSNs by blocking information under typical strategies. Based on the existing two-player Stackelberg zero-sum game model and evaluation methods of node’s importance on the network, we analyze the typical strategic dynamic game in which the blocker moves first and the spreader moves later on scale-free networks with different power exponent. Experimental results show that the absolute dominance strategy of the blocker is Leader Rank with 90.16% probability. And using Leader Rank can be relatively effective against malicious spreaders with 98.33% probability. When the power exponent of the network is smaller, it is more conducive to blocking information dissemination with fewer seed nodes.
On the hardness of quadratic unconstrained binary optimization problems
Authors: Mehta V. , Jin F. , Michielsen K. , De Raedt H.
Published: August 2022

We use exact enumeration to characterize the solutions of quadratic unconstrained binary optimization problems of less than 21 variables in terms of their distributions of Hamming distances to close-by solutions. We also perform experiments with the D-Wave Advantage 5.1 quantum annealer, solving many instances of up to 170-variable, quadratic unconstrained binary optimization problems. Our results demonstrate that the exponents characterizing the success probability of a D-Wave annealer to solve a quadratic unconstrained binary optimization correlate very well with the predictions based on the Hamming distance distributions computed for small problem instances.
GaInSn liquid nanospheres as a saturable absorber for an Er:CaF2 laser at 2.75 μm
Authors: Chen Bo , Li Kuan , Jin Yicheng , Wang Peifu , Zhang Ning , Zhang Ke , Liu Shande , Xu Jinlong
Published: August 2022

High-quality GaInSn liquid nanospheres are successfully fabricated by the ultrasonic method as a novel saturable absorber in the mid-infrared range. An open-aperture Z-scan technique is applied to study the saturation absorption property, presenting a modulation depth of 34.3% and a saturable fluence of 0.497 GW/cm2 at 2.3 μm, respectively. With GaInSn nanospheres as a saturable absorber, a stable Q-switched Er:CaF2 crystal laser operating at 2.75 μm is realized. The maximum Q-switched output power of 361 mW is obtained under the absorbed pump power of 2.9 W. The shortest pulse width of 500 ns and the highest repetition rate of 67 kHz are generated, corresponding to maximum peak power and single pulse energy of 10.78 W and 5.39 μJ, respectively. These findings indicate a promising potential of GaInSn nanospheres SA for generating nanosecond mid-infrared laser pulses.
Pre-shot combinations and game-related statistics discriminating between winners and losers depending on the game location during the NBA COVID-19 season
Authors: Bustamante-Sánchez Alvaro , Gomez-Ruano Miguel-Angel , Clemente-Suárez Vicente Javier , Jiménez-Sáiz Sergio L.
Published: August 2022

Basketball in performance depends on numerous factors, where a stable trend was identified by winning teams with better performances in shooting effectiveness and rebounding. However, there is a need for a better understanding of pre-shot combinations that lead to these performance trends. This study aimed to analyze NBA teams’ game-related statistics, pre-shooting combinations, and pick-and-roll differences between winning and losing teams (considering the context: playing at home, away, or in a neutral court) during the COVID-19 season. A retrospective cross-sectional study on the 2019–2020 NBA season (906 games) was carried out. Game-related statistics were gathered from the private company InStat (https://basketball.instatscout.com/). The discriminant analysis and binary logistic regression models were run in order to discriminate the most important features of winning teams depending on the game location. The results showed that defensive rebounds and three-point shooting percentage remained the most important variables that best discriminated winners and losers independently of the game location context. The main results showed that winning teams had a better shooting percentage based on three-pointers, catch-and-shot actions, cuts, pick-and-roll efficacy, and uncontested shots based on a better collective behavior after a successful space creation dynamic through a tactical functional unit. At the same time, teams would need players with the ability to clear those possessions in which the opponents force to an isolation or a contested shot. From a practical application perspective, coaches should focus on composing a team with good shooters, skilled players in isolations, and a good game-time pick-and-roll strategy.
Effects of rearing systems on the eggshell quality, bone parameters and expression of genes related to bone remodeling in aged laying hens
Authors: Fu Yu , Wang Jing , Schroyen Martine , Chen Gang , Zhang Hai-jun , Wu Shu-geng , Li Bao-ming , Qi Guang-hai
Published: August 2022

Public concerns regarding animal welfare are changing the selection of rearing systems in laying hens. This study investigated the effects of rearing systems on eggshell quality, bone parameters and relative expression levels of genes related to bone remodeling in aged laying hens. A total of 2,952 55-day-old Jing Tint Six pullets were randomly assigned to place in the conventional caging system (CCS) or aviary system (AVS) and kept until 95 weeks of age. The AVS group delayed the decrease of eggshell quality and alleviated the symptoms of osteoporosis in the humerus rather than in the femur. Eggshell breaking strength, thickness, weight, weight ratio, stiffness and fracture toughness were decreased linearly with age (from 55 to 95 weeks of age, p < 0.05). The AVS group had higher eggshell breaking strength, stiffness and fracture toughness than the CCS group (p < 0.05). Higher total calcium and phosphorus per egg were presented in the AVS group at 95 weeks of age (p < 0.05). At 95 weeks of age, the AVS group had a humerus with higher weight, volume, length, midpoint perimeter, cortical index, fat-free dry weight, ash content, total calcium per bone, total phosphorus per bone, average bone mineral density, strength, stiffness and work to fracture compared to the CCS group (p < 0.05). Such differences did not appear in the femur. The relative expression levels of alkaline phosphatase (ALP) and osteocalcin (OCN) genes in the femur and hormone receptors (vitamin D receptor (VDR), estrogen receptor alpha (ERα) and fibroblast growth factor 23 (FGF23)) genes in the humerus were significantly upregulated (p < 0.05) in the AVS group. The level of tartrate-resistant acid phosphatase (TRAP) transcripts was also increased (p < 0.05) in the femur of the AVS group. Overall, compared with the CCS, the AVS alleviated the deterioration of eggshell and bone qualities of aged laying hens, which may be related to the changes in the expression of genes associated with bone remodeling.
Palmelloid formation in the Antarctic psychrophile, Chlamydomonas priscuii, is photoprotective
Authors: Szyszka-Mroz Beth , Ivanov Alexander G. , Trick Charles G. , Hüner Norman P. A.
Published: August 2022

Cultures of the obligate, Antarctic psychrophile, Chlamydomonas priscuii grown at permissive low temperature (8°C) are composed of flagellated, single cells, as well as non-motile, multicellular palmelloids. The relative proportions of the two cell types are temperature dependent. However, the temperature dependence for palmelloid formation is not restricted to psychrophilic C. priscuii but appears to be a general response of mesophilic Chlamydomonas species (C. reinhardtii and C. raudensis) to non-permissive growth temperatures. To examine potential differences in photosynthetic performance between single cells versus palmelloids of the psychrophile, a cell filtration technique was developed to separate single cells from palmelloids of C. priscuii grown at 8°C. Flow cytometry was used to estimate the diameter of isolated single cells (≤5 μm) versus isolated palmelloids of varying size (≥8 μm). Compared to single cells, palmelloids of C. priscuii showed a decrease in the abundance of light-harvesting complex II (LHCII) proteins with a 2-fold higher Chl a/b ratio. A decrease in both lutein and β-carotene in palmelloids resulted in carotenoid pools which were 27% lower in palmelloids compared to single cells of the psychrophile. Chlorophyll fluorescence analyses of the isolated fractions revealed that maximum photochemical efficiency of PSII (Fv/Fm) was comparable for both single cells and palmelloids of C. priscuii. However, isolated palmelloids exhibited lower excitation pressure, measured as 1 - qL, but higher yield of PSII (ΦPSII) and 50% higher rates of electron transport (ETR) than single cells exposed to high light at 8°C. This decreased sensitivity to high light in isolated palmelloids compared to single cells was associated with greater non-regulated dissipation of excess absorbed energy (ΦNO) with minimal differences in ΦNPQ in C. priscuii in response to increasing irradiance at low temperature. The ratio ΦNO/ΦNPQ observed for isolated palmelloids of C. priscuii developed at 8°C (1.414 ± 0.036) was 1.38-fold higher than ΦNO/ΦNPQ of isolated single cells (1.021 ± 0.018) exposed to low temperature combined with high light (1,000 μmol m−2 s−1). The differences in the energy quenching capacities between palmelloids and single cells are discussed in terms of enhanced photoprotection of C. priscuii palmelloids against low-temperature photoinhibition.
The relationship between anxiety, enjoyment, and breakdown fluency during second language speaking tasks: An idiodynamic investigation
Authors: Aubrey Scott
Published: August 2022

Research has found that levels of enjoyment and anxiety fluctuate on a moment-to-moment timescale during second language (L2) spoken task performances as learners attempt to cope with various communication challenges. For L2 speakers, surges in these emotions can limit or expand cognitive resources, affecting speech processing capability. However, at an intra-individual level, there is very little empirical evidence on how emotions and fluency are related during L2 spoken task performances. The present study uses the idiodynamic approach to examine the relationship between enjoyment, anxiety, and breakdown fluency (i.e., average length of pauses) during monolog tasks performed by university students who use English as an L2. After watching a video recording of their task performances, participants rated their anxiety and emotion levels on a per-second basis. Immediately after, they were interviewed about their attributions for fluctuations in their ratings. After segmenting task performances into 26 7-s segments of speech, per-person correlations revealed that (1) the (negative) relationship between anxiety and enjoyment varied from strong to very weak, and (2) the (positive) relationship between anxiety and breakdown fluency was much stronger than the (negative) relationship between enjoyment and breakdown fluency. Triangulation of anxiety and enjoyment ratings, stimulated recall interviews, and performance data led to the identification of four categories of factors that influenced the emotion-fluency relationship: task design factors (task structure, task topic), task implementation factors (pre-task planning, task time limits), cognitive-linguistic factors (momentary breakdowns in conceptualization and formulation speech processes), and achievement outcome factors (self-evaluations of appropriate and inappropriate language used).
Flurbiprofen used in one-lung ventilation improves intraoperative regional cerebral oxygen saturation and reduces the incidence of postoperative delirium
Authors: Shen Liang , Chen Jia-qi , Yang Xin-lu , Hu Ji-cheng , Gao Wei , Chai Xiao-qing , Wang Di
Published: August 2022

Background

We previously demonstrated that flurbiprofen increased arterial oxygen partial pressure and reduced intrapulmonary shunts. The present study aims to investigate whether flurbiprofen improves intraoperative regional cerebral oxygen saturation (rScO2) and reduces the incidence of postoperative delirium (POD) in elderly patients undergoing one-lung ventilation (OLV).

Methods

One hundred and twenty patients undergoing thoracoscopic lobectomy were randomly assigned to the flurbiprofen-treated group (n = 60) and the control-treated group (n = 60). Flurbiprofen was intravenously administered 20 minutes before skin incision. The rScO2 and partial pressure of arterial oxygen (PaO2) were recorded during the surgery, and POD was measured by the Confusion Assessment Method (CAM) within 5 days after surgery. The study was registered in the Chinese Clinical Trial Registry with the number ChiCTR1800020032.

Results

Compared with the control group, treatment with flurbiprofen significantly improved the mean value of intraoperative rScO2 as well as the PaO2 value (P < 0.05, both) and significantly reduced the baseline values of the rScO2 area under threshold (AUT) (P < 0.01) at 15, 30, and 60 min after OLV in the flurbiprofen-treated group. After surgery, the POD incidence in the flurbiprofen-treated group was significantly decreased compared with that in the control group (P < 0.05).

Conclusion

Treatment with flurbiprofen may improve rScO2 and reduce the incidence of POD in elderly patients undergoing thoracoscopic one-lung ventilation surgery for lung cancer.

Clinical trial registration

http://www.chictr.org/cn/, identifier ChiCTR1800020032.
Understanding patient and physician responses to various cost-sharing programs for prescription drugs in South Korea: A multilevel analysis
Authors: Son Kyung-Bok , Lee Eui-Kyung , Lee Sang-Won
Published: August 2022

Introduction

Patient and/or physician responses are a pivotal issue in designing rational cost-sharing programs under health insurance systems.

Objectives

This study aims to understand patient and/or physician responses to cost-sharing programs designed for prescription drugs in South Korea.

Methods

As a framework, we took advantage of a tiered cost-sharing program, including from copayment to coinsurance (threshold 1) and reduced coinsurance (threshold 2). Given the hierarchical structure of prescriptions nested within patients, we utilized a multilevel analysis to assess effects of various cost-sharing programs on patient and/or physician responses using National Health Insurance claims data from 2018.

Results

We found that a tiered cost-sharing program was effective in changing the behaviors of patients and/or physicians. Threshold 1 was found to be more effective than threshold 2 in changing their behaviors. At the prescription level, sensitivity to cost-sharing programs was associated with prescribed days of treatment and locations of prescription. In a similar vein, sensitivity to cost-sharing programs was associated with gender and age group of patients.

Conclusion

A simplified cost-sharing program with extended intervals should be considered to rationalize cost-sharing programs. Specifically, a cost-sharing program designed for long-term prescriptions for chronic diseases together with an emphasis on cost transparency is required to better guide price-conscious decisions by patients and/or physicians.
How and when perceived COVID-19 crisis strength impacts individuals' life satisfaction and sleep quality: A moderated mediation model
Authors: Lan Yuanyuan , Han Changlin , Liu Xiaotong , Cao Qinqin , Chen Siyuan , Xia Yuhuan
Published: August 2022

The COVID-19 pandemic has caused millions of deaths, seriously hampering people's lives and their productivity. Drawing on social information processing theory, this research developed a moderated mediation model to investigate the influence of perceived COVID-19 crisis strength on individuals' well-being. The results from a sample of 441 suggest that individuals' perceived COVID-19 crisis strength indirectly affects their life satisfaction and sleep quality via their perceived risk of being infected. Moreover, both individuals' trust in local government and mindfulness trait can buffer the positive effect of perceived COVID-19 crisis strength on their perceived risk of being infected. At the same time, they also buffer the indirect impact of individuals' perceived COVID-19 crisis strength on life satisfaction and sleep quality through perceived risk of being infected. This research provides several practical implications for governments and individuals to mitigate the negative influences of the COVID-19 pandemic and help individuals boost life satisfaction and sleep quality.
Determination of dielectric properties and predictive modeling for designing radio-frequency heating of ground beef
Authors: Purohit Anuj , Kumari Ankanksha , Roy Anupam , Mohan Anand
Published: August 2022

Dielectric properties of ground beef as affected by the variation of fat content (73/27, 80/20, and 85/15 lean/fat) and temperature (0–90 °C) were determined using a parallel plate LCR meter and liquid test fixture at the radiofrequency (RF) of 27.12 MHz. The values of dielectric constant (ε′) and dielectric loss factor (ε'') of ground beef significantly increased with temperature (p < 0.05) whereas the values of penetration depth (dp) decreased with temperature (p < 0.05) up to the respective points of local extrema. Values of both ε′ and ε'' were significantly lower (p < 0.05) for the leaner blend 85/15 and conversely, penetration depth was significantly higher (p < 0.05) for the leaner blend. As temperature increased beyond the point of maximum for ε′ and ε'' and point of minimum for dp, there was no significant difference in the dielectric properties between the different lean/fat blends. Predictive equations of second order in temperature and fat content were developed for the dielectric properties which explained 74, 93, and 98% variation in ε′, ε'', and for dp respectively, based on R2 values. The dielectric properties and their fitted models can be utilized for designing pilot and mass scale RF processes for heating ground beef.
Case report: 18F-FDG PET confirmed pupil-sparing third nerve palsy heralding aseptic cavernous sinus embolism in patient with chest malignancy
Authors: Xiong Jianmei , Liu Huanbo , Li Jianyong , Hou Jiajia , Cui Fang
Published: August 2022

Classical cavernous sinus embolism is a rare clinical finding, presented most commonly by complaints of headache, diplopia, visual field defects, facial pain, and progressive neurological deficits. Many patients exhibit symptoms of III, IV, and VI nerve palsies. We hereby report a rare case of aseptic cavernous sinus embolism developed in a 75-year-old male with primary lung cancer who presented with binocular diplopia due to unilateral third and sixth cranial nerve palsies with pupil-sparing. The possibility of cavernous sinus cancer embolus should be considered if the routine examination excluded metastases, infiltration, carcinomatous meningitis, or the paraneoplastic process. 18F-FDG PET imaging may provide a promising diagnostic modality for the diagnosis of cancer embolus.
Unusually large paraganglioma complicated with successive catecholamine crises: A case report and review of the literature
Authors: Huang Zhenhui , Liang Guojian , Shen Hua , Hong Chuyuan , Yin Xuexia , Zhang Shi
Published: August 2022

Background

Paragangliomas are rare neuroendocrine tumors that could secret catecholamines. Hypertension and heart failure caused by the catecholamine crisis are fatal cardiovascular events. However, silent paragangliomas that lack typical symptoms of catecholamine pose a significant diagnostic challenge.

Case summary

A 45-year-old woman who presented with more than 1-year history of abdominal discomfort was suspected of having a gastrointestinal stromal tumor by a local hospital since a vast metastatic mass occupied her left abdomen. Thus, she was recommended to our hospital. After completing the gastroscopy, she unexpectedly developed acute heart failure and was transferred to the Intensive Care Unit (ICU) where the initial diagnosis of paraganglioma was considered through path. However, a second catecholamine crisis due to constipation led to acute heart failure again. After anti-heart failure therapy and rigorous preoperative preparation, surgery was arranged to remove the tumor. Postoperative pathology conﬁrmed the paraganglioma, and the patient was discharged from the hospital in good condition.

Conclusion

We reported a rare case of huge retro-peritoneal paraganglioma with successive catecholamine crises and acute heart failure. This was probably the largest retro-peritoneal paraganglioma since the 1980s. Besides, we were the first to use surgical drawing to illustrate its complex anatomical adjacent relationship of retro-peritoneal paraganglioma. Our case emphasizes the inclusion of extra-adrenal paraganglioma in the differential diagnosis of retroperitoneal tumors. In suspected paragangliomas, catecholamine testing is preferable to invasive procedures including gastroscopy and biopsy to avoid triggering a catecholamine crisis. Surgical resection is the primary treatment. We highlight the priority of dealing with the venous reflux branches of the tumor to prevent the release of catecholamines into the blood. In particular, preoperative preparation plays a vital role in managing paraganglioma. Moreover, it is necessary to schedule genetic testing and clinical follow-up due to the metastatic potential of paragangliomas.
Identification of risk factors for 1-year mortality among critically ill older adults with hip fractures surgery: A single medical center retrospective study
Authors: Luo Taijun , Zhang Juxia , Zhou Haibin , Xu Tao , Zhang Wenchao , Wang Geng
Published: August 2022

Aim

The purpose of this study was to analyze the potential risk factors for mortality 1 year after hip fracture surgery in critically ill older adults.

Methods

We reviewed 591 critically ill older adults who underwent hip surgery at our institution from January 2018 to April 2021. We collected baseline demographics, clinical information, and 1-year survival status of the sample patients by means of medical record systems and follow-up phone calls. Patients were divided into survival and mortality groups based on survival within 1 year after surgery.

Results

Based on the results of the 1-year postoperative follow-up of patients, we obtained 117 cases in the death group and 474 cases in the survival group, and this led to a 1-year mortality rate of 19.8% (117/591) after hip fracture in critically ill older adults at our hospital. The risk factors that influenced the 1-year postoperative mortality were identified as advanced age (HR:1.04, 95%, 1.01–1.06), preoperative arrhythmia (HR: 1.95, 95%, 1.26–2.70), high level of NLR (HR:1.03, 95%, 1.01–1.06), respiratory failure (HR: 2.63, 95%, 1.32–5.23), and acute cardiovascular failure. 5.23) and acute cardiovascular events (HR: 1.65, 95%, 1.05–2.59).

Conclusion

Advanced age, preoperative arrhythmias, high levels of NLR, postoperative respiratory failure, and acute cardiovascular events were independent risk factors for survival of critically ill older adults with hip fracture at 1 year after surgery. Therefore, laboratory tests such as high levels of preoperative NLR can be an important indicator of patient prognosis.