bluuebunny/crossref_metadata_2025 huggingface dataset:
A Survey of Current Activities and Technologies Used to Detect Carbapenem Resistance in Bacteria Isolated from Companion Animals at Veterinary Diagnostic Laboratories—United States, 2020
Authors: Waltenburg Michelle A. , Shugart Alicia , Loy John Dustin , Tewari Deepanker , Zhang Shuping , Cole Stephen D. ,   CRO Veterinary Diagnostic Laboratory Investigation Group, Walters Maroya Spalding , Nichols Megin
Published: January 2022

Carbapenems are antimicrobial drugs reserved for the treatment of severe multidrug-resistant Gram-negative bacterial infections. Carbapenem-resistant organisms (CROs) are an urgent public health threat and have been made reportable to public health authorities in many jurisdictions. Recent reports of CROs in companion animals and veterinary settings suggest that CROs are a One Health problem. However, standard practices of U.S. veterinary diagnostic laboratories (VDLs) to detect CROs are unknown.
Attenuation of Getah Virus by a Single Amino Acid Substitution at Residue 253 of the E2 Protein that Might Be Part of a New Heparan Sulfate Binding Site on Alphaviruses
Authors: Wang Ningning , Zhai Xiaofeng , Li Xiaoling , Wang Yu , He Wan-Ting , Jiang Zhiwen , Veit Michael , Su Shuo
Published: January 2022

Due to decades of inadequate monitoring and lack of vaccines and specific treatment, a large number of people have been infected with alphaviruses. GETV is a re-emerging alphavirus that has the potential to infect humans.
Human Milk Oligosaccharides Reduce Murine Group BStreptococcusVaginal Colonization with Minimal Impact on the Vaginal Microbiota
Authors: Mejia Marlyd E. , Ottinger Samantha , Vrbanac Alison , Babu Priyanka , Zulk Jacob J. , Moorshead David , Bode Lars , Nizet Victor , Patras Kathryn A.
Published: January 2022

During pregnancy, GBS ascension into the uterus can cause fetal infection or preterm birth. In addition, GBS exposure during labor creates a risk of serious disease in the vulnerable newborn and mother postpartum.
Contrasting Effects of Local Environmental and Biogeographic Factors on the Composition and Structure of Bacterial Communities in Arid Monospecific Mangrove Soils
Authors: Thomson T. , Fusi M. , Bennett-Smith M. F. , Prinz N. , Aylagas E. , Carvalho S. , Lovelock C. E. , Jones B. H. , Ellis J. I.
Published: January 2022

Mangrove ecosystems are increasingly being recognized for their potential to sequester atmospheric carbon, thereby mitigating the effects of anthropogenically driven greenhouse gas emissions. The bacterial community in the soils plays an important role in the breakdown and recycling of carbon and other nutrients.
Novel DNA Markers for Identification of Actinobacillus pleuropneumoniae
Authors: Srijuntongsiri Gun , Mhoowai Atiwat , Samngamnim Sukuma , Assavacheep Pornchalit , Bossé Janine T. , Langford Paul R. , Posayapisit Navaporn , Leartsakulpanich Ubolsree , Songsungthong Warangkhana
Published: January 2022

Species-specific markers are crucial for infectious disease diagnostics. Mutations within a marker sequence can lead to false-negative results, inappropriate treatment, and economic loss.
Differentiating Trypanosoma cruzi in a Host Mammalian Cell Imaged in Aqueous Liquid by Atmospheric Scanning Electron Microscopy
Authors: Takagi Yuko , Sato Mari , Naya Masami , Sato Chikara
Published: January 2022

Using Atmospheric Scanning Electron Microscopy (ASEM), we visualized interaction between infectious stage of
Trypanosoma cruzi
and completely intact host mammalian cell. Plasma membrane appears translucent under ASEM, which not only enables direct observation of
T. cruzi
within its host cell, but also reveals internal structures of the parasite itself.
Generation of Distinct Differentially Culturable Forms of
Burkholderia
following Starvation at Low Temperature
Authors: Auty Joss M. , Jenkins Christopher H. , Hincks Jennifer , Straatman-Iwanowska Anna A. , Allcock Natalie , Turapov Obolbek , Galyov Edouard E. , Harding Sarah V. , Mukamolova Galina V.
Published: January 2022

Bacterial pathogens exhibit physiologically distinct forms that enable their survival in an infected host, the environment and following exposure to antimicrobial agents.
B. pseudomallei
causes the disease melioidosis, which has a high mortality rate and is difficult to treat with antibiotics. The bacterium is endemic to several countries and detected in high abundance in the environment.
Two-pronged kill mechanism at the end-Triassic mass extinction
Authors: Fox Calum P. , Whiteside Jessica H. , Olsen Paul E. , Cui Xingqian , Summons Roger E. , Idiz Erdem , Grice Kliti
Published: January 2022

High-resolution biomarker and compound-specific isotope distributions coupled with the degradation of calcareous fossil remnants reveal that intensive euxinia and decalcification (acidification) driven by Central Atlantic magmatic province (CAMP) activity formed a two-pronged kill mechanism at the end-Triassic mass extinction. In a newly proposed extinction interval for the basal Blue Lias Formation (Bristol Channel Basin, UK), biomarker distributions reveal an episode of persistent photic zone euxinia (PZE) that extended further upward into the surface waters. In the same interval, shelly taxa almost completely disappear. Beginning in the basal paper shales of the Blue Lias Formation, a Lilliput assemblage is preserved consisting of only rare calcitic oysters (Liostrea) and ghost fossils of decalcified aragonitic bivalves. The stressors of PZE and decalcification parsimoniously explain the extinction event and inform possible combined causes of other biotic crises linked to emplacement of large igneous provinces, notably the end-Permian mass extinction, when PZE occurred on a broad and perhaps global scale.
Effect of renin-angiotensin system blocker on COVID-19 in young patients with hypertension
Authors: Kuzeytemiz Mustafa , Tenekecioglu Erhan
Published: January 2022

Hypertension is found frequently in patients with COVID-19 and is often treated with ACE inhibitors (ACEIs) and angiotensin receptor blockers (ARBs). SARS-CoV-2, the pathogen of COVID-19, binds to the receptors of ACE2 to enter the alveolar cells, raising questions on whether these drugs are salutary or harmful with respect to any propensity for COVID-19 or to disease prognosis. We investigated the impact of ACEI/ARB and the clinical prognosis of patients with hypertension with COVID-19. In this study, 250 patients with hypertension (<45 years old) with COVID-19 were recruited. None of these patients had any chronic disease except for hypertension. The study population was grouped according to antihypertensive medication: ACEI/ARB user and non-ACEI/ARB user. Patients were followed for clinical prognosis and biochemical and radiological findings during their hospital stay. Adverse cardiovascular event (myocardial infarction, all-cause death, stroke), transfer to the intensive care unit, severity of symptoms during the treatment course, length of hospital stay and effort capacity in the treadmill stress test were recorded. During hospital stay, there was no significant difference in terms of length of hospital stay, medication for COVID-19, left ventricular ejection fraction on echocardiography and metabolic equivalents in the treadmill stress test between patients treated with and without ACEI/ARB. During treatment of COVID-19, there was no significant difference in clinical adverse event, effort capacity and clinical course between patients with and without ACEI/ARB. It appears that patients with COVID-19 may continue to use ACEI/ARB or that ACEI/ARB may be added safely to their antihypertensive treatment.
Approximation Algorithms for the Capacitated Min–Max Correlation Clustering Problem
Authors: Ji Sai , Li Jun , Wu Zijun , Xu Yicheng
Published: January 2022

In this paper, we propose a so-called capacitated min–max correlation clustering model, a natural variant of the min–max correlation clustering problem. As our main contribution, we present an integer programming and its integrality gap analysis for the proposed model. Furthermore, we provide two approximation algorithms for the model, one of which is a bi-criteria approximation algorithm and the other is based on LP-rounding technique.
Shear strain-induced structure relaxation of Ni Σ17 [110](223) grain boundary: A molecular dynamics simulation
Authors: Xue Hongtao , Yu Xudong , Zhou Xin , Tang Fuling , Li Xiuyan , Wu Youzhi , Ren Junqiang , Lu Xuefeng
Published: January 2022

The stabilization of grain boundaries (GBs) is beneficial for improving the stability and mechanical properties of nanocrystalline (NC) metals. Molecular dynamics (MD) calculations were performed to investigate the shear response of Ni [Formula: see text]17 [110](223) symmetrical tilt GB. It was found that under the action of shear, the nucleation and evolution of the GB source Shockley partial dislocations ultimately result in the low-energy-state transformation of the GB structure units (SUs). However, the Ag atom contained in the GB increases the shear stress and strain required for the GB relaxation, and the strain range for the GB relaxation is expanded, indicating the inhibitory effect of the Ag atom on the structural relaxation of Ni [Formula: see text]17 [110](223) GB. As the temperature increases from 10 K to 250 K, the structural relaxation of Ni [Formula: see text]17 [110](223) GB becomes easier to proceed. In addition to segregation-induced GB stabilization, strain-induced GB relaxation and the roles of foreign atom and temperature clarified in this work could provide several new entry points for stabilizing high-energy GBs.
A Contract Service Provider Model for Virtual Assets
Authors: Hardjono Thomas , Lipton Alexander , Pentland Alex
Published: January 2022

With the recent rise in the cost of transactions on blockchain platforms, there is a need to explore other service models that may provide a more predictable cost to customers and end-users. We discuss the Contract Service Provider (CSP) model as a counterpart of the successful Internet Service Provider (ISP) model. Similar to the ISP business model based on peered routing-networks, the CSP business model is based on multiple CSP entities forming a CSP Community or group offering a contract service for specific types of virtual assets. We discuss the contract domain construct which encapsulates well-defined smart contract primitives, policies and contract-ledger. We offer a number of design principles borrowed from the design principles of the Internet architecture.
The Influence of Boundaries on Gravity Currents and Thin Films: Drainage, Confinement, Convergence, and Deformation Effects
Authors: Zheng Zhong , Stone Howard A.
Published: January 2022

Thin film flows, whether driven by gravity, surface tension, or the relaxation of elastic boundaries, occur in many natural and industrial processes. Applications span problems of oil and gas transport in channels to hydraulic fracture, subsurface propagation of pollutants, storage of supercritical CO2 in porous formations, and flow in elastic Hele–Shaw configurations and their relatives. We review the influence of boundaries on the dynamics of thin film flows, with a focus on gravity currents, including the effects of drainage into the substrate, and the role of the boundaries to confine the flow, force its convergence to a focus, or deform, and thus feedback to alter the flow. In particular, we highlight reduced-order models. In many cases, self-similar solutions can be determined and describe the behaviors in canonical problems at different timescales and length scales, including self-similar solutions of both the first and second kind. Additionally, the time transitions between different solutions are summarized. Where possible, remarks about various applications are provided.
Women in Electrochemistry- Contributions, Challenges and Potential Solutions
Authors: Das Ananya , Nagaraj Pratham , Bhat Panemangalore Devadas
Published: January 2022

The perspectives gained over the years by women working in electrochemistry are described in order to throw light on their history and current status and achievements in this field, the potential that the future holds, and the role that well-established female electrochemists and the electrochemical societies can play in improving upon the under-representation and under-recognition of women in electrochemistry. Here, a hopeful and optimistic future is presented, in which men and women, both equally contribute to this field, which encompasses our entire life, from corrosion and life of materials to transportation industry.
Electrochemical Determination of Famotidine in Real Samples Using rGO/Cu2O Nanocomposite Modified Carbon Paste Electrode
Authors: Afruz Ali , Amiri Mandana , Imanzadeh Hamideh
Published: January 2022

Here, we have developed a sensitive electrochemical sensor for famotidine (FAT) using Cu2O nanoparticles and reduced graphene oxide (rGO). The Cu2O nanoparticles and rGO were synthesized through a simple process and characterized by versatile analytical techniques. The prepared Cu2O nanoparticles and rGO were taken to modify the carbon paste electrode (Cu2O/rGO/CPE) and applied for the electrochemical analysis of the FAT at pH 6.0. Cu2O/rGO/CPE showed superior response to determine FAT, attributed to the high surface area of rGO and the electrocatalytic properties of Cu2O nanoparticles. The designed FAT sensor exhibited two dynamic linear ranges from 0.1–3.0 μM and 3.0–50.0 μM with a limit of detection of 0.08 μM (S/N = 3) using a differential pulse voltammetry. The proposed sensor also showed a repeatable and stable response over one month with negligible interference from usual organic and inorganic species. The sensor was also validated to measure FAT in real samples (urine, serum and pharmaceutical tablet) with good recoveries from 99.6 to 110.9%.
Integration of Semi-Empirical and Artificial Neural Network (ANN) for Modeling Lithium-Ion Electrolyte Systems Dynamic Viscosity
Authors: Azeez Fadhel , Refaie Abdelrahman
Published: January 2022

The dynamic viscosity is a key characteristic of electrolyte performance in a Lithium-ion (Li-ion) battery. This study introduces a one-parameter semi-empirical model and artificial neural network (ANN) to predict the viscosity of salt-free solvent mixtures and relative viscosity of Li-ion electrolyte solutions (lithium salt + solvent mixture), respectively. Data used in this study were obtained experimentally, in addition to data extracted from literature. The ANN model has seven inputs: salt concentration, electrolyte temperature, salt-anion size, solvent melting, boiling temperatures, solvent dielectric constant, and solvent dipole moment. Different configurations of the ANN model were tested, and the configuration with the least error was chosen. The results show the capability of the semi-empirical model in predicting the viscosity with an overall mean absolute percentage error (MAPE) of 2.05% and 3.17% for binary and tertiary mixtures, respectively. The ANN model predicted the relative viscosity of electrolyte solutions with MAPE of 4.86%. The application of both models in series predicted the viscosity with MAPE of 2.3%; however, the ANN MAPE alone is higher than this value. Thus, this study highlights the promise of using predictive models to complement physical approaches and effectively perform initial screening on Li-ion electrolytes.
Review—Metal-Organic Framework-Based Supercapacitors
Authors: Wang Zhengyun , Zhong Yanyu , Wei Chenhuinan , Jiang Lipei , Liu Hongfang
Published: January 2022

Supercapacitors as a class of energy storage devices possess high power density, outstanding reversibility and long cycle life. The development of innovative electrode materials and unconventional configurations determine the successful operation of supercapacitors. Owing to controllable porous structure and unique electrochemical behavior, great efforts have been devoted to designing and fabricating innovative metal-organic frameworks (MOFs) based supercapacitors. In this review, recent developments in MOFs related supercapacitors electrodes are summarized. Furthermore, the advances in MOFs composites for supercapactiors application including pristine MOFs, MOFs composites and MOFs-derived composites are systematically discussed by integrating charge storing principle. Finally, future prospects and opportunities in the design of MOFs related electrodes are also delineated.
Remedy for Collapse of ZIF Derived Carbon: Micro-Pore Filled Synthesis to Improve ORR Catalytic Property
Authors: Wu Xingxing , Zhang Songwei , Gao Jiaojiao , Liu Xiaopeng , Yuan Qunhui , Wu Yiying , Gan Wei
Published: January 2022

Zeolitic imidazolate framework (ZIF) derived carbons deliver outstanding performance as oxygen reduction reaction (ORR) catalysts. However, their electrocatalytic activities are limited due to unavoidable collapse of ZIFs upon pyrolysis, which results in degradation of porosity, sintering of metals and loss of active sites. In this work, a micro-pore filling strategy was employed to strength the architecture of ZIF by using size matched cyanamide molecules as fillers. The cyanamide with high nitrogen content shows a triple effect in stabilizing the carbonaceous skeleton, preserving of metal containing active sites and improving the conductivity of matrix. Therefore, the as-prepared Fe, Co co-doped ZIF derived carbon (FeCo@NC-N) delivers a significantly improved electrochemical activity for ORR than its unfilled counterpart, with half-wave potential upshifted by 30 mV (0.84 V vs RHE). Besides, a promoted power density of home-assembled zinc-air battery is obtained when FeCo@NC-N is applied as cathode catalyst. This work demonstrates a reliable approach to mitigate framework collapse of metal organic framework (MOF), thus may open a new way for fabrication of MOF based catalysts with increased loading of pores and active sites.
Intravital imaging reveals inflammation as a dominant pathophysiology of age-related hepatovascular changes
Authors: Vats Ravi , Li Ziming , Ju Eun-Mi , Dubey Rikesh K. , Kaminski Tomasz W. , Watkins Simon , Pradhan-Sundd Tirthadipa
Published: January 2022

Aging is the most significant risk factor for the majority of chronic diseases, including liver disease. The cellular, molecular, and pathophysiological mechanisms that promote age-induced hepatovascular changes are unknown due to our inability to visualize changes in liver pathophysiology in live mice over time. We performed quantitative liver intravital microscopy (qLIM) in live C57BL/6J mice to investigate the impact of aging on the hepatovascular system over a 24-mo period. qLIM revealed that age-related hepatic alterations include reduced liver sinusoidal blood flow, increased sinusoidal vessel diameter, and loss of small hepatic vessels. The ductular cell structure deteriorates with age, along with altered expression of hepatic junctional proteins. Furthermore, qLIM imaging revealed increased inflammation in the aged liver, which was linked to increased expression of proinflammatory macrophages, hepatic neutrophils, liver sinusoidal endothelial cells, senescent cells, and procoagulants. Finally, we detected elevated NF-κB pathway activity in aged livers. Overall, these findings emphasize the importance of inflammation in age-related hepatic vasculo-epithelial alterations and highlight the utility of qLIM in studying age-related effects in organ pathophysiology.
Developmental alterations of intestinal SGLT1 and GLUT2 induced by early weaning coincides with persistent low-grade metabolic inflammation in female pigs
Authors: Li Yihang , Thelen Kyan M. , Fernández Karina Matos , Nelli Rahul , Fardisi Mahsa , Rajput Mrigendra , Trottier Nathalie L. , Contreras Genaro A. , Moeser Adam J.
Published: January 2022

These studies reveal that early-life adversity in the form of early weaning in pigs causes a developmental shift in intestinal glucose transport from SGLT1 toward GLUT2-mediated transport. Early weaning also induced markers of metabolic inflammation including persistent elevations in blood glucose and the inflammatory marker CRP, along with increased visceral adiposity. Altered intestinal glucose transport might contribute to increased risk for inflammatory and metabolic diseases associated with early-life adversity.
Characteristics of mesenteric adipose tissue attached to different intestinal segments and their roles in immune regulation
Authors: Zhang Haowei , Ding Yujin , Zeng Qin , Wang Dandan , Liu Ganglei , Hussain Zain , Xiao Boen , Liu Wei , Deng Tuo
Published: January 2022

Our results change the paradigm of how we regard MAT as a contiguous and homogeneous tissue to an intensely heterogeneous tissue. Appreciation of the differences between regional MATs will guide future research to investigate the specialized roles of different MATs in intestinal health and disease.
Exposure to binge ethanol and fatty acid ethyl esters exacerbates chronic ethanol-induced pancreatic injury in hepatic alcohol dehydrogenase-deficient deer mice
Authors: Srinivasan Mukund P. , Bhopale Kamlesh K. , Caracheo Anna A. , Kaphalia Lata , Gong Bin , Popov Vsevolod L. , Boor Paul J. , Shakeel Ansari G. A. , Kaphalia Bhupendra S.
Published: January 2022

We established a chronic EtOH feeding model of hepatic alcohol dehydrogenase-deficient (ADH−) deer mice, which mimics several fibroinflammatory features of human alcoholic chronic pancreatitis (ACP). The fibroinflammatory and morphological features exacerbated by chronic plus binge EtOH and FAEEs exposure provide a strong case for metabolic basis of ACP. Most importantly, several pathological and molecular targets identified in this study provide a much broader understanding of the mechanism and avenues to develop therapeutics for ACP.
The profibrotic and senescence phenotype of old lung fibroblasts is reversed or ameliorated by genetic and pharmacological manipulation of Slc7a11 expression
Authors: Ritzenthaler Jeffrey D. , Torres-Gonzalez Edilson , Zheng Yuxuan , Zelko Igor N. , van Berkel Victor , Nunley David R. , Kidane Biniam , Halayko Andrew J. , Summer Ross , Watson Walter H. , Roman Jesse
Published: January 2022

Increased senescence and expression of profibrotic genes in old lung fibroblasts contribute to disrepair responses. We reported that primary lung fibroblasts from old mice have lower expression and activity of the cystine transporter Slc7a11/xCT than cells from young mice, resulting in changes in both the intracellular and extracellular redox environments. This study examines the hypothesis that low Slc7a11 expression in old lung fibroblasts promotes senescence and profibrotic gene expression. The levels of mRNA and protein of Slc7a11, senescence markers, and profibrotic genes were measured in primary fibroblasts from the lungs of old (24 mo) and young (3 mo) mice. In addition, the effects of genetic and pharmacological manipulation of Slc7a11 were investigated. We found that decreased expression of Slc7a11 in old cells was associated with elevated markers of senescence (p21, p16, p53, and β-galactosidase) and increased expression of profibrotic genes (Tgfb1, Smad3, Acta2, Fn1, Col1a1, and Col5a1). Silencing of Slc7a11 in young cells replicated the aging phenotype, whereas overexpression of Slc7a11 in old cells decreased expression of senescence and profibrotic genes. Young cells were induced to express the senescence and profibrotic phenotype by sulfasalazine, a Slc7a11 inhibitor, whereas treatment of old cells with sulforaphane, a Slc7a11 inducer, decreased senescence without affecting profibrotic genes. Like aging cells, idiopathic pulmonary fibrosis fibroblasts show decreased Slc7a11 expression and increased profibrotic markers. In short, old lung fibroblasts manifest a profibrotic and senescence phenotype that is modulated by genetic or pharmacological manipulation of Slc7a11.
Cocaine increases stimulation-evoked serotonin efflux in the nucleus accumbens
Authors: Yuen Jason , Goyal Abhinav , Rusheen Aaron E. , Kouzani Abbas Z. , Berk Michael , Kim Jee Hyun , Tye Susannah J. , Blaha Charles D. , Bennet Kevin E. , Lee Kendall H. , Oh Yoonbae , Shin Hojin
Published: January 2022

Serotonin plays a vital role in drug addiction. Here, using N-shaped fast-scan cyclic voltammetry, we demonstrated the effect of cocaine on the phasic release of serotonin at the nucleus accumbens core. To the best of our knowledge, this has not previously been elucidated. Our results not only reinforce the role of serotonin in the mechanism of action of cocaine but also help to fill a gap in our knowledge and provide a baseline for future studies in cocaine addiction.
Methylation Pattern Mediated by m6A Regulator and Tumor Microenvironment Invasion in Lung Adenocarcinoma
Authors: Jiang Feng , Hu Yifang , Liu Xiaoqin , Wang Ming , Wu Chuyan
Published: January 2022

Background. Recent research has established the existence of epigenetic modulation of the immune response. The possible involvement of RNA-n6-methyladenosine (m6A) alteration in tumor microenvironment (TME) cell invasion, on the other hand, is unknown. Methods. Based on 23 m6A regulators, we examined the alteration patterns of m6A in 629 LUAD tissues and comprehensively connected these modification patterns with TME cell invasion characteristics. The m6A score was calculated, and the m6A modification pattern of a single tumor was quantified using principal component analysis. Then, we further verified the expression of m6A related enzymes and the role hub gene (NOL10) closely related to survival in lung cancer cell lines. Results. Three separate m6A alteration modes have been discovered. TME cell invasion characteristics in the three modes were very similar to the three immunological phenotypes of tumors: immunological rejection, immunological inflammation, and immunological desert. We show that assessing the m6A modification pattern in a single tumor may help predict tumor inflammatory stage, subtype, TME interstitial activity, and prognosis. TME phenotypic inflammation is indicated by a high m6A score, which is characterized by elevated mutation load and immunological activation. The low m6A subtype showed matrix activation and ineffective immune infiltration, indicating that the TME phenotype of noninflammation and immunological rejection had a poor survival probability. Increased neoantigen burden was also linked to a high m6A score. Patients with a higher m6A score saw substantial therapeutic and clinical improvements. And reducing hub gene NOL10 expression substantially inhibited lung cancer cell growth and migration. Conclusions. This research shows that m6A alteration is critical in the creation of TME variety and complexity. The analysis of a single tumor’s m6A alteration pattern will aid in improving our knowledge of TME invasion features and guiding more effective immunotherapy tactics.
Question Text Classification Method of Tourism Based on Deep Learning Model
Authors: Luo Wanli , Zhang Lei
Published: January 2022

The Internet of Things applications are diverse in nature, and a key aspect of it is multimedia sensors and devices. These IoT multimedia devices form the Internet of Multimedia Things (IoMT). Compared with the Internet of Things, it generates a large amount of text data with different characteristics and requirements. Aiming at the problems that machine learning and single structure deep learning model cannot effectively grasp the text emotional information in text processing, resulting in poor classification effect, this paper proposes a text classification method of tourism questions based on deep learning model. First, the corpus is trained with word2vec tool based on continuous word bag model to obtain the text word vector representation. Then, the attention mechanism is introduced into the long-short term network (LSTM), and the attention-based LSTM model is constructed for text feature extraction, which highlights the impact of different words in the input text on the text emotion category. Finally, the text features are input into the Softmax classifier to obtain the probability distribution of text categories, and the model is trained combined with the cross entropy loss function. The experimental results show that the average accuracy, recall, and

F

value are 0.943, 0.867, and 0.903, respectively, which has better classification effect than other methods.
Research on Intelligent Scheduling Mechanism in Edge Network for Industrial Internet of Things
Authors: Zhang Zhenzhong , Sun Wei , Yu Yanliang
Published: January 2022

With the vigorous development of the Internet of Things, the Internet, cloud computing, and mobile terminals, edge computing has emerged as a new type of Internet of Things technology, which is one of the important components of the Industrial Internet of Things. In the face of large-scale data processing and calculations, traditional cloud computing is facing tremendous pressure, and the demand for new low-latency computing technologies is imminent. As a supplementary expansion of cloud computing technology, mobile edge computing will sink the computing power from the previous cloud to a network edge node. Through the mutual cooperation between computing nodes, the number of nodes that can be calculated is more, the types are more comprehensive, and the computing range is even greater. Broadly, it makes up for the shortcomings of cloud computing technology. Although edge computing technology has many advantages and has certain research and application results, how to allocate a large number of computing tasks and computing resources to computing nodes and how to schedule computing tasks at edge nodes are still challenges for edge computing. In view of the problems encountered by edge computing technology in resource allocation and task scheduling, this paper designs a dynamic task scheduling strategy for edge computing with delay-aware characteristics, which realizes the reasonable utilization of computing resources and is required for edge computing systems. This paper proposes a resource allocation scheme combined with the simulated annealing algorithm, which minimizes the overall performance loss of the system while keeping the system low delay. Finally, it is verified through experiments that the task scheduling and resource allocation methods proposed in this paper can significantly reduce the response delay of the application.
The Laplacian Spectrum, Kirchhoff Index, and the Number of Spanning Trees of the Linear Heptagonal Networks
Authors: Liu Jia-Bao , Chen Jing , Zhao Jing , Wang Shaohui
Published: January 2022

Let Hn be the linear heptagonal networks with 2n heptagons. We study the structure properties and the eigenvalues of the linear heptagonal networks. According to the Laplacian polynomial of Hn, we utilize the method of decompositions. Thus, the Laplacian spectrum of Hn is created by eigenvalues of a pair of matrices: LA and LS of order numbers 5n + 1 and 4n + 1n!/r! (n − r)!, respectively. On the basis of the roots and coefficients of their characteristic polynomials of LA and LS, we get not only the explicit forms of Kirchhoff index but also the corresponding total number of spanning trees of Hn.
The Effect of 3D Image Virtual Reconstruction Based on Visual Communication
Authors: Xu Li , Bai Ling , Li Lei
Published: January 2022

Considering the problems of poor effect, long reconstruction time, large mean square error (MSE), low signal-to-noise ratio (SNR), and structural similarity index (SSIM) of traditional methods in three-dimensional (3D) image virtual reconstruction, the effect of 3D image virtual reconstruction based on visual communication is proposed. Using the distribution set of 3D image visual communication feature points, the feature point components of 3D image virtual reconstruction are obtained. By iterating the 3D image visual communication information, the features of 3D image virtual reconstruction in visual communication are decomposed, and the 3D image visual communication model is constructed. Based on the calculation of the difference of 3D image texture feature points, the spatial position relationship of 3D image feature points after virtual reconstruction is calculated to complete the texture mapping of 3D image. The deep texture feature points of 3D image are extracted. According to the description coefficient of 3D image virtual reconstruction in visual communication, the virtual reconstruction results of 3D image are constrained. The virtual reconstruction algorithm of 3D image is designed to realize the virtual reconstruction of 3D image. The results show that when the number of samples is 200, the virtual reconstruction time of this paper method is 2.1 s, and the system running time is 5 s; the SNR of the virtual reconstruction is 35.5 db. The MSE of 3D image virtual reconstruction is 3%, and the SSIM of virtual reconstruction is 1.38%, which shows that this paper method can effectively improve the ability of 3D image virtual reconstruction.
Yijin-Tang Attenuates Cigarette Smoke and Lipopolysaccharide-Induced Chronic Obstructive Pulmonary Disease in Mice
Authors: Rho Jinhyung , Seo Chang-Seob , Hong Eun-Ju , Baek Eun Bok , Jung Eunhye , Park Suyoung , Lee Mee-Young , Kwun Hyo-Jung
Published: January 2022

Background. Chronic obstructive pulmonary disease (COPD) refers to a lung disorder associated with symptoms of dyspnea, cough, and sputum production. Traditionally, Yijin-tang (YJT), a mixture of Pinellia ternate, Poria cocos, ginger, Chinese liquorice, and tangerine peel, has been prescribed for the treatment of respiratory system diseases caused by dampness phlegm. This experiment investigated the therapeutic effect of YJT in a mouse model of cigarette smoke (CS)- and lipopolysaccharide (LPS)-induced COPD. Methods. COPD was induced by exposing mice to CS for 1 hour per day for 8 weeks, with intranasal delivery of LPS on weeks 1, 3, 5, and 7. YJT was administered at doses of 100 and 200 mg/kg 1 hour before CS exposure for the last 4 weeks. Results. YJT significantly suppressed CS- and LPS-induced increases in inflammatory cell counts and reduced interleukin-1 beta (IL-1β), IL-6, tumor necrosis factor-alpha (TNF-α), and monocyte chemoattractant protein-1 (MCP-1) levels in bronchoalveolar lavage fluid (BALF) and lung tissue. In addition, YJT not only decreased airway wall thickness, average alveolar intercept, and lung fibrosis, but it also suppressed the expression of matrix metallopeptidase (MMP)-7, MMP-9, and transforming growth factor-B (TGF-β) and collagen deposition. Moreover, YJT suppressed phosphorylation of nuclear factor-kappa B (NF-κB) as well as expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Conclusion. Collectively, our findings show that YJT attenuates respiratory inflammation and airway remodeling caused by CS and LPS exposure; therefore, therapeutic applications in COPD can be considered.
Experimental Study on the Effect of Freezing and Thawing on the Shear Strength of the Frozen Soil in Qinghai‐Tibet Railway Embankment
Authors: Wang B. , Gao J. H. , Wang Y. Q. , Quan X. J. , Gong Y. W. , Zhou S. W.
Published: January 2022

The direct shear tests of different dry density and moisture content samples at different temperatures of the frozen soil in the Qinghai‐Tibet Railway embankment between Tanggula South and Anduo section were carried out to analyze the influence rules of each experimental factor on the mechanical properties of frozen soil during the freeze‐thaw process. The results show the following. (1) When the frozen soil temperature is below 0°C and continues to drop during the freezing and thawing process, each sample shows the law of a significant increase in cohesion and a slight decrease in the internal friction angle. In the meantime, the cohesion obtained during the thawing process of the sample at the same temperature point is higher than that obtained during the freezing process. In contrast, the internal friction angles exhibit an opposite law, where the internal friction angle during the melting process is lower than the internal friction angle during the freezing process. After freezing‐thawing action, it deserves to be mentioned that the cohesion increases slightly while the internal friction angles present a slight decrease trend compared to the initial state. (2) With the decrease in temperature and the gradual increase in cohesion, the temperature curve can be divided into a fast‐growing section from 0 to −2°C, a slow‐growing section from −2 to −8°C, and a second fast‐growing section from −8 to −10°C owing to the combined effect of the pressure‐thawing action and ice‐water phase change. In addition, the rate of decrease in the internal friction angle also shows a similar pattern. (3) The cohesion and the internal friction angle of samples both tend to increase first and then decrease with the rise of the initial moisture content, and the critical initial moisture content is near the optimal moisture content of 15%. (4) Both the cohesion and the internal friction angle of the samples increase with dry density growth. The growth rate of cohesion will gradually increase as the temperature decreases. Moreover, the growth rate of cohesion of low dry density samples is more susceptible to temperature, while the internal friction angle growth rate is not affected by temperature.
DOK5 as a Prognostic Biomarker of Gastric Cancer Immunoinvasion: A Bioinformatics Analysis
Authors: Luo Fengyong , Wang Zhihuai , Chen Shuai , Luo Zhenbo , Wang Gaochao , Yang Haojun , Tang Liming
Published: January 2022

Background. Docking protein 5 (DOK5) is a member of the docking protein group of membrane proteins and is an adapter protein involved in signal transduction. Nevertheless, the role of DOK5 expression in the prognosis of gastric cancer (GC) remains unclear. Methods. In this study, clinical prognostic parameters and survival data related to DOK5, in patients with GC, were analyzed using bioinformatics analysis comprising Oncomine and TIMER, UALCAN database, Kaplan‐Meier plotter, GEPIA, GSEA, DAVID, and cBioPortal websites. Results. In our study, GC contained various DOK5 expressions, which forecasted poor survival outcomes. Moreover, our research showed that high DOK5 could predict high‐level infiltration of several GC immune cells, as evidenced by M1, TAM, M2, B cell, and T cell failure. Hence, DOK5 might become a new gastric cancer biomarker and therapeutic target. In the following analysis, in order to explore the prognostic value of DOK5 in GC, more clinical trials are needed to validate our results. Conclusions. Through multiple database verifications, DOK5 was found to be part of the pathogenic genes for GC. Thus, it can change the formation and progression of tumors by acting on human immunity.
Algo Huele mal en la Industria del Gas
Authors: Saavedra Eduardo
Published: January 2022

Indignación causó en todos los chilenosuna serie de abusos revelados por un estudio de la Fiscalía Nacional Económica (FNE) que se producen transversalmenteen toda la industria del gas, en particular aquellos que ocurren en el mercado de distribución mayorista del gas licuado de petróleo (GLP) que se vende en balones y aquellos abusos que involucran a la principal empresa distribuidora de gas natural en Chile. Es conveniente revisar por qué es importante para los usuarios lo que sucede en estos mercados, en qué consisten exactamente los abusos detectados, cuál es su alcance en términos de posibles sanciones en tribunales y, más relevante aún, qué decisiones se pueden o deben tomar a nivel de gobierno y parlamento para corregir el mal funcionamiento de estos mercados. Continuar leyendo...
Metabolomic Biomarkers in Blood Samples Identify Cancers in a Mixed Population of Patients with Nonspecific Symptoms
Authors: Larkin James R. , Anthony Susan , Johanssen Vanessa A. , Yeo Tianrong , Sealey Megan , Yates Abi G. , Smith Claire Friedemann , Claridge Timothy D.W. , Nicholson Brian D. , Moreland Julie-Ann , Gleeson Fergus , Sibson Nicola R. , Anthony Daniel C. , Probert Fay
Published: January 2022

Purpose:

Early diagnosis of cancer is critical for improving patient outcomes, but cancers may be hard to diagnose if patients present with nonspecific signs and symptoms. We have previously shown that nuclear magnetic resonance (NMR) metabolomics analysis can detect cancer in animal models and distinguish between differing metastatic disease burdens. Here, we hypothesized that biomarkers within the blood metabolome could identify cancers within a mixed population of patients referred from primary care with nonspecific symptoms, the so-called “low-risk, but not no-risk” patient group, as well as distinguishing between those with and without metastatic disease.

Experimental Design:

Patients (n = 304 comprising modeling, n = 192, and test, n = 92) were recruited from 2017 to 2018 from the Oxfordshire Suspected CANcer (SCAN) pathway, a multidisciplinary diagnostic center (MDC) referral pathway for patients with nonspecific signs and symptoms. Blood was collected and analyzed by NMR metabolomics. Orthogonal partial least squares discriminatory analysis (OPLS-DA) models separated patients, based upon diagnoses received from the MDC assessment, within 62 days of initial appointment.

Results:

Area under the ROC curve for identifying patients with solid tumors in the independent test set was 0.83 [95% confidence interval (CI): 0.72–0.95]. Maximum sensitivity and specificity were 94% (95% CI: 73–99) and 82% (95% CI: 75–87), respectively. We could also identify patients with metastatic disease in the cohort of patients with cancer with sensitivity and specificity of 94% (95% CI: 72–99) and 88% (95% CI: 53–98), respectively.

Conclusions:

For a mixed group of patients referred from primary care with nonspecific signs and symptoms, NMR-based metabolomics can assist their diagnosis, and may differentiate both those with malignancies and those with and without metastatic disease.

See related commentary by Van Tine and Lyssiotis, p. 1477
Phase I Study of High-Dose l-Methylfolate in Combination with Temozolomide and Bevacizumab in Recurrent IDH Wild-Type High-Grade Glioma
Authors: Salas Lucas A. , Stewart Thomas G. , Mobley Bret C. , Peng Chengwei , Liu Jing , Loganathan Sudan N. , Wang Jialiang , Ma Yanjun , Berger Mitchel S. , Absher Devin , Hu Yang , Moots Paul L. , Christensen Brock C. , Clark Stephen W.
Published: January 2022

Isocitrate dehydrogenase (IDH) mutations in low-grade gliomas (LGG) result in improved survival and DNA hypermethylation compared with IDH wild-type LGGs. IDH-mutant LGGs become hypomethylated during progression. It is uncertain whether methylation changes occur during IDH wild-type GBM progression and whether the methylome can be reprogrammed. This phase I study evaluated the safety, tolerability, efficacy, and methylome changes after l-methylfolate (LMF) treatment, in combination with temozolomide and bevacizumab in patients with recurrent high-grade glioma. Fourteen patients total, 13 with GBM, one with anaplastic astrocytoma, all IDH wild-type were enrolled in the study. All patients received LMF at either 15, 30, 60, or 90 mg daily plus temozolomide (75 mg/m2 5 days per month) and bevacizumab (10 mg/kg every two weeks).No MTD was identified. LMF-treated patients had median overall survival of 9.5 months [95% confidence interval (CI), 9.1–35.4] comparable with bevacizumab historical control 8.6 months (95% CI, 6.8–10.8). Six patients treated with LMF survived more than 650 days. Across all treatment doses, the most adverse events were diarrhea (7%, 1 patient, grade 2), reflux (7%, 1 patient, grade 2), and dysgeusia (7%, 1 patient, grade 2). In the six brains donated at death, there was a 25% increase in DNA methylated CpGs compared with the paired initial tumor. LMF in combination with temozolomide and bevacizumab was well tolerated in patients with recurrent IDH wild-type high-grade glioma. This small study did not establish a superior efficacy with addition of LMF compared with standard bevacizumab therapy; however, this study did show methylome reprogramming in high-grade glioma.

Significance:

Glioblastoma (GBM) is a primary brain tumor with a poor prognosis. Therapies to date have failed to improve survival. LGGs, with IDH mutations, have increased global DNA methylation and increased survival compared with GBMs. GBMs lack this mutation and have less DNA methylation. Here we show that the DNA methylome can be modified in GBM with LMF. Such treatment might be useful in methylome priming prior to immunotherapy.
Bartsocas-Papas Syndrome: Case Report
Authors: Nikhat Fareeda , Fernandes Shalini Dolly , Mashharawi Nabilah , Bahutair Shadha
Published: January 2022

Bartsocas-Papas syndrome (BPS) is a rare form of autosomal recessive disease, which is often severe and most of the time lethal. Only a few cases have been reported in the literature, so far mainly from the Mediterranean ancestry. We are describing here a case of severe form of BPS, which we encountered in our hospital in Dubai, UAE, which was diagnosed by antenatal scan; the baby had typical features of BPS and died immediately within few hours of life. Parents were first-degree consanguineous couples, and there was a family history of this disorder.
COMP (Cartilage Oligomeric Matrix Protein), a Novel PIEZO1 Regulator That Controls Blood Pressure
Authors: Wang Hui , Yuan Ze , Wang Bianbian , Li Bochuan , Lv Huizhen , He Jinlong , Huang Yaqian , Cui Zhen , Ma Qiannan , Li Ting , Fu Yi , Tan Xiaoli , Liu Yangping , Wang Shengpeng , Wang Changhe , Kong Wei , Zhu Yi
Published: January 2022

Background:

Vascular endothelial cells are critical for maintaining blood pressure (BP) by releasing biologically active molecules, such as nitric oxide. A non-endothelial cell resident matricellular protein, COMP (cartilage oligomeric matrix protein), plays a pivotal role in maintaining cardiovascular homeostasis, but little is known about its regulatory effect on BP.

Methods:

Mice were infused with AngII (angiotensin II; 450 ng/kg per minute) for 3 days via an osmotic minipump, and BP was monitored by a tail-cuff system. Second-order mesenteric arteries were isolated from mice for microvascular tension measurement. Nitric oxide was detected by an electron paramagnetic resonance technique. Small-interfering RNA transfection, co-immunoprecipitation, bioluminescence resonance energy transfer assays, and patch-clamp electrophysiology experiments were used for further detailed mechanism investigation.

Results:

COMP
−/−
mice displayed elevated BP and impaired acetylcholine-induced endothelium-dependent relaxation compared with wild-type mice with or without AngII. Inhibition of eNOS (endothelial nitric oxide synthase) abolished the difference in endothelium-dependent relaxation between wild-type and COMP
−/−
mice. Furthermore, COMP directly interacted with the C-terminus of Piezo1 via its C-terminus and activated the endogenous Piezo1 currents, which induced intracellular Ca
2+
influx, Ca
2+
/calmodulin-dependent protein kinase type II and eNOS activation, and nitric oxide production. The Piezo1 activator, Yoda1, reduced the difference in endothelium-dependent relaxation and BP in wild-type and COMP
−
/−
mice. Moreover, COMP overexpression increased eNOS activation and improved endothelium-dependent relaxation and BP.

Conclusions:

Our study demonstrated that COMP is a novel Piezo1 regulator that plays a protective role in BP regulation by increasing cellular Ca
2+
influx, eNOS activity, and nitric oxide production.