bluuebunny/crossref_metadata_2025 huggingface dataset:
A Bi-Level Optimization Approach to Network Flow Management Incorporating Travelers’ Herd Effect
Authors: Li Shihao , Zhou Bojian , Xu Min , Dong Xiaoxiao
Published: December 2024

Herd effect is a widespread phenomenon in real-world situations. This study explores how the herd effect can be used to manage network flow effectively. We examined its impact on travelers’ route choices and propose a mixed network flow evolution process that incorporates the herd effect, considering two types of travelers: those who receive route subsidy information and those who do not. Based on this evolution process, we developed a bi-level optimization model to determine the optimal subsidized routes, the subsidy amounts per kilometer, and the proportion of travelers receiving subsidy information. A hybrid algorithm with two iterative procedures was proposed to solve the model, in which the adaptive genetic algorithm (AGA) was employed to solve the upper-level nonlinear mixed-integer programming problem, and the partial linearization method was used to solve the lower-level network flow evolution process. Numerical results indicate that the presence of herd effect can effectively reduce both the total travel time of the network and the overall subsidy costs. The findings of this study have significant implications for the utilization of the herd effect in designing navigation software and developing congestion pricing strategies.
Two Families of Bi-Univalent Functions Associating the (p, q)-Derivative with Generalized Bivariate Fibonacci Polynomials
Authors: Swamy Sondekola Rudra , Frasin Basem Aref , Breaz Daniel , Cotîrlă Luminita-Ioana
Published: December 2024

Making use of generalized bivariate Fibonacci polynomials, we propose two families of regular functions of the type ϕ(ζ)=ζ+∑j=2∞djζj, which are bi-univalent in the disc {ζ∈C:|ζ|<1} involving the (p, q)-derivative operator. We find estimates on the coefficients |d2|, |d3| and the of Fekete–Szegö functional for members of these families. Relevant connections to the existing results and new consequences of the main result are presented.
Exploring Reverse Sural Flap Necrosis in Lupus-like Syndrome: Challenges and Strategies in Lower Limb Reconstruction—A Case Presentation
Authors: Ceccaroni Alessandra , Cuomo Roberto , Pentangelo Paola , Gentile Antonioenrico , Marra Caterina , Rozen Warren Matthew , Seth Ishith , Lim Bryan , Alfano Carmine
Published: December 2024

Soft tissue reconstruction in the lower limbs presents a significant challenge, particularly when addressing defects in the distal third of the leg, ankle, and foot. The reverse sural flap reliant on the perforating branches of the peroneal artery has emerged as a versatile option, offering a solution for patients for whom microsurgical techniques are not feasible. Despite its advantages, the procedure carries inherent risks, especially in populations with underlying conditions, such as venous insufficiency, cardiovascular disease, and diabetes, as well as in elderly patients, where the likelihood of flap necrosis is elevated. This report details a case of reverse sural flap necrosis in a patient with lupus-like syndrome, a complex scenario that underscores the need for meticulous preoperative assessment and planning. The case illustrates not only the technical considerations and challenges associated with the reverse sural flap but also the broader implications of systemic autoimmune disorders on postoperative outcomes. Through a comprehensive review of the literature, we explore the relationship between vascularization, autoimmune profiles, and the success of reverse sural flap procedures. We highlight the critical need for surgeons to adopt a holistic approach to patient evaluation, considering both local and systemic factors that may influence the viability of the flap and the overall reconstructive success.
Polysulfone-Based Membranes Modified with Ionic Liquids and Silica for Potential Fuel Cell Applications
Authors: Fernández-Llamazares Emma , Nguyen Thi Hai Van , Verdugo Pere , Gual Aitor , Garcia Diogo M. E. , Delgado Simão Claudia , Díaz de los Bernardos Miriam , Nogalska Adrianna
Published: December 2024

The urgent need for sustainable, low-emission energy solutions has positioned proton exchange membrane fuel cells (PEMFCs) as a promising technology in clean energy conversion. Polysulfone (PSF) membranes with incorporated ionic liquid (IL) and hydrophobic polydimethylsiloxane-functionalized silica (SiO2-PDMS) were developed and characterized for their potential application in PEMFCs. Using a phase inversion method, membranes with various combinations of PSFs, SiO2-PDMS, and 1-butyl-3-methylimidazolium triflate (BMI.TfO) (1–10 wt%) were prepared and characterized to assess their morphology, porosity, wettability, ionic conductivity, and thermal stability. Incorporating IL significantly altered the membrane structure, increasing porosity and surface roughness, while SiO2-PDMS enhanced IL retention, reducing leakage by up to 32%. Proton conductivity increased by up to 30 times compared to pure PSF, and membranes exhibited high hydrophilicity at optimal IL concentrations. This work highlights the potential of IL and silica-based membranes for practical applications in PEMFCs.
Influence of Structural Parameters on the Mechanical Performance of Multi-Layer U-Shaped Metal Bellows
Authors: Fu Pengcheng , Yu Gaochao , Yang Shida , Zhou Meiling , Qi Shaocong , Meng Qingdang , Jia Shangwu , Zhao Jun
Published: December 2024

Metal bellows feature a simple structure, high-temperature resistance, corrosion resistance, and strong flexibility for compensation, making them widely used in the aerospace, machinery, and petrochemical industries. Compared to multilayer bellows, single-layer bellows are simpler in structure and forming process, making the performance easier to achieve. The structural parameters of multilayer metal bellows, particularly the number of layers, significantly impact the performance. This study focuses on multilayer U-shaped metal bellows made of 304 stainless steel. Using ABAQUS finite element software, a full simulation of the hydroforming and performance analysis of multilayer U-shaped metal bellows is conducted. This study examines the effects of wall thickness thinning and residual stress distribution caused by hydroforming and explores how structural parameters (including outer diameter, corrugation height, corrugation spacing, and wall thickness) influence axial stiffness and bending performance. The findings provide valuable insights for the design and selection of metal bellows.
The Role of Diet, Additives, and Antibiotics in Metabolic Endotoxemia and Chronic Diseases
Authors: Park Ji-Eun , Park Ho-Young , Kim Young-Soo , Park Miri
Published: December 2024

Background/Objectives: Dietary patterns, including high-fat and high-carbohydrate diets (HFDs and HCDs), as well as non-dietary factors such as food additives and antibiotics, are strongly linked to metabolic endotoxemia, a critical driver of low-grade chronic inflammation. This review explores the mechanisms through which these factors impair intestinal permeability, disrupt gut microbial balance, and facilitate lipopolysaccharide (LPS) translocation into the bloodstream, contributing to metabolic disorders such as obesity, type 2 diabetes mellitus, and inflammatory bowel disease. Methods: The analysis integrates findings from recent studies on the effects of dietary components and gut microbiota interactions on intestinal barrier function and systemic inflammation. Focus is given to experimental designs assessing gut permeability using biochemical and histological methods, alongside microbiota profiling in both human and animal models. Results: HFDs and HCDs were shown to increase intestinal permeability and systemic LPS levels, inducing gut dysbiosis and compromising barrier integrity. The resulting endotoxemia promoted a state of chronic inflammation, disrupting metabolic regulation and contributing to the pathogenesis of various metabolic diseases. Food additives and antibiotics further exacerbated these effects by altering microbial composition and increasing gut permeability. Conclusions: Diet-induced alterations in gut microbiota and barrier dysfunction emerge as key mediators of metabolic endotoxemia and related disorders. Addressing dietary patterns and their impact on gut health is crucial for developing targeted interventions. Further research is warranted to standardize methodologies and elucidate mechanisms for translating these findings into clinical applications.
Modeling Electrowetting on Dielectric for Novel Droplet-Based Microactuation
Authors: Parsi Behzad , Gunn Max R. , Winters Jacob V. , Maynes Daniel , Crane Nathan B.
Published: December 2024

Recent advancements in Electrowetting on Dielectric (EWOD) systems, such as simplified fabrication, low-voltage actuation, and the development of more reliable materials, are expanding the potential applications of electrowetting actuators. One application of EWOD actuators is in RF devices to enable dynamic reconfiguration and allow real-time adjustments to frequency and bandwidth. In this paper, a method is introduced to actuate a panel using EWOD forces. In the EWOD system, the velocity of the plate increases by maximizing the actuation force, minimizing the moving mass (droplets and metalized plate), and reducing resistance (contact line drag, fluid drag). However, some of these are competing factors. For instance, the actuation force can be increased by increasing the number of droplets, but this also increases the inertia and the drag force. An analytical model of EWOD actuation is presented to understand system performance tradeoffs. The model is validated with an EWOD experiment, and the data demonstrate less than a 7.8% error between the measured and predicted maximum plate velocities for different voltage inputs. In addition, this study presents a 3D numerical FEM model to analyze the velocity profile and viscous force in the thin droplets, focusing on variations along the droplet’s height, which cannot be captured experimentally. The main advantage of the proposed system over previous works is the simple 2D manufacturing process, which allows embedding metalized plates and RF circuit boards, in addition to being compact, portable, and low-cost. In addition, the proposed method does not have any mechanical components, which can increase the system’s reliability in a harsh environment.
Deformation-Induced Electromagnetic Reconfigurable Square Ring Kirigami Metasurfaces
Authors: Fan Xuanqing , Pan Zijian , Zhu Yunfan , Li Min , Ma Yunpeng , Li Yuhang
Published: December 2024

The continuous expansion of wireless communication application scenarios demands the active tuning of electromagnetic (EM) metamaterials, which is essential for their flexible adaptation to complex EM environments. However, EM reconfigurable systems based on intricate designs and smart materials often exhibit limited flexibility and incur high manufacturing costs. Inspired by mechanical metastructures capable of switching between multistable configurations under repeated deformation, we propose a planar kirigami frequency selective surface (FSS) that enables mechanical control of its resonant frequency. This FSS is composed of periodically arranged copper square-ring resonators embedded in a kirigami-structured ecoflex substrate. Through simple tensile deformation, the shapes and positions of the square-ring resonators on the kirigami substrate are altered, resulting in changes to the coupling between capacitance and inductance, thereby achieving active tuning. Combining EM finite element simulations and transmittance measurements, we demonstrate that biaxial mechanical stretching allows for continuous adjustment of the FSS resonant frequency and −10 dB bandwidth. Additionally, the FSS exhibits excellent polarization and incident angle stability. Structural parameterization of the square-ring kirigami FSS was conducted to elucidate the deformation–electromagnetic coupling mechanism underlying the active tuning. These insights provide a foundation for guiding the application of square-ring kirigami FSS in various practical engineering domains.
A Meta-Analysis of the Human Gut Mycobiome Using Internal Transcribed Spacer Data
Authors: Zhang Zeming , Zhang Yining , Yuan Qixiang , Wang Zuoyi , Hu Songnian , Yin Peng , He Zilong
Published: December 2024

The intestinal mycobiome is closely related to human health. There have been several reports investigating the association between the gut fungi and disease, but there is still a lack of overall assessment of the human gut mycobiome. Here, we performed a meta-analysis based on 2372 ITS (Internal Transcribed Spacer) data collected publicly online. We found that the mycobiome diversity of human gut fungi varies significantly across diseases by using EasyAmplicon, and these fungi are mainly composed of three genera, Saccharomyces, Candida, and Aspergillus. In addition, we performed the construction of disease prediction models based on ITS data by using the random forest model and verified the generalization ability of the models. We hope that our results will provide strong support for subsequent studies of the intestinal mycobiome.
The Degradation Characteristics and Soil Remediation Capabilities of the Butachlor-Degrading Strain DC-1
Authors: Cheng Yue , Fu Qian , Xiong Guixin , Huang Yaning , Li Xu , Yu Qingyue , He Fuxia , Li Haitao , Liu Rongmei
Published: December 2024

Butachlor is a widely utilized acetamide herbicide noted for its systemic selectivity against pre-emergence grass weeds. Butachlor has negative effects on organisms and the environment, so it is necessary to screen degradation strains. In this investigation, Bacillus cereus strain DC-1 was isolated from soil persistently exposed to butachlor. Through rigorous single-factor and response surface analyses, strain DC-1 exhibited a notable 87.06% degradation efficiency under optimized conditions where the temperature was 32.89 °C, pH was 7.29, and inoculum concentration was 5.18%. It was further hypothesized by LC-MS that the degradation pathway of butachlor by strain DC-1 might be as follows: butachlor undergoes initial deoxygenation catalyzed by dioxygenases to form 2-chloro-N-(2,6-diethylphenyl)-N-methylacetamide, followed by N-demethylation yielding 2-chloro-N-(2,6-diethylphenyl) acetamide, and culminating in conversion to 2,6-diethylphenol. In addition, bioremediation experiments of butachlor-contaminated soil were conducted. The results show that strain DC-1 could degradable 99.23% of butachlor (100 mg·kg−1) from the soil within 12 d, and soil sucrase, cellulase, and urease activities are promoted by the bacteria. And through high-throughput sequencing, it was concluded that the strain DC-1 was able to influence the relative abundance of certain bacteria in the soil, and make the microbial community in the soil develop in a more stable and beneficial direction. DC-1 thus represents a valuable resource in the realm of butachlor degradation due to its robust efficacy, favorable characteristics, and ecological restorative capabilities, underscoring its promising role in the bioremediation of butachlor-contaminated soils.
Cryptosporidium Infection During Pregnancy and Effects on Pregnancy Outcomes in Israel
Authors: Shapiro Ben David Shirley , Snitser Olga , Parizade Miriam , Orvieto Noam , Segal Yaacov , Adler Limor
Published: December 2024

Cryptosporidium is a parasite that typically causes self-limited gastroenteritis. Little is known about the course of infection and its impact during pregnancy. This retrospective cohort study conducted in Israel assessed the effects of Cryptosporidium infection on pregnancy and obstetrical outcomes. The study population included pregnant women with a positive PCR stool test for Cryptosporidium and a control group of pregnant women with negative tests, matched at a 3:1 ratio based on age, gestational week, and sector. Their medical records were retrieved for symptoms, treatments, pregnancy termination, gestational age, birth weight, Apgar score, and head circumference. Fisher’s exact or chi-squared tests were used to determine significance. Between January 2020 and December 2023, 2512 pregnant women underwent PCR stool testing. Of these, 93 were positive for Cryptosporidium, mostly in 2022. Their median age was 31 (range: 23–42 years), and 77.4% were from medium–high socioeconomic status. The most common symptom was diarrhea (90/93, 96.7%). The infection was self-limiting in all cases, with none requiring specific treatment. No significant differences were found in miscarriage rate, delivery week, birth weight, Apgar score, or head circumference compared to the controls. This study illuminates the clinical course of Cryptosporidium infection in pregnant women, emphasizing a generally favorable outcome without the necessity for therapeutic intervention.
NgLst8 Coactivates TOR Signaling to Activate Photosynthetic Growth in Nannochloropsis gaditana
Authors: Zhang Zhengying , Yang Shu , Li Yanyan , Xie Dian , Chen Guobin , Ren Jiaxu , Zhu Hongmei , Zhou Hantao
Published: December 2024

The target of rapamycin (TOR) serves as a central regulator of cell growth, coordinating anabolic and catabolic processes in response to nutrient availability, growth factors, and energy supply. Activation of TOR has been shown to promote photosynthesis, growth, and development in yeast, animals, and plants. In this study, the complete cDNA sequence of the Lst8 gene was obtained from Nannochloropsis gaditana. The structure of N. gaditana LST8 comprises a typical WD40 repeat sequence, exhibiting high sequence similarity to several known LST8 proteins. By overexpressing the Lst8 gene in N. gaditana, we constructed the NgLst8 transgenic algal strain and measured its photosynthetic activity and growth. We observed that an increase in LST8 abundance promotes the expression of TOR-related kinase, thereby enhancing photosynthetic growth. Transcriptome analysis further elucidated the response mechanism of elevated Lst8 abundance in relation to photosynthesis. Our findings indicate that increased Lst8 expression activates ABC transporter proteins and the MAPK signaling pathway, which regulate the transmembrane transport of sugars and other metabolites, integrate photosynthesis, sugar metabolism, and energy signaling, and modulate energy metabolism in algal cells through interactions with the TOR signaling pathway.
Correction: Drago et al. Bacteria Living in Biofilms in Fluids: Could Chemical Antibiofilm Pretreatment of Culture Represent a Paradigm Shift in Diagnostics? Microorganisms 2024, 12, 259
Authors: Drago Lorenzo , Fidanza Andrea , Giannetti Alessio , Ciuffoletti Alessio , Logroscino Giandomenico , Romanò Carlo Luca
Published: December 2024

In the original publication [...]
The Use of Chemical Flocculants and Chitosan as a Pre-Concentration Step in the Harvesting Process of Three Native Microalgae Species from the Canary Islands Cultivated Outdoors at the Pilot Scale
Authors: Figueira Garcia Laura , Gojkovic Zivan , Venuleo Marianna , Guidi Flavio , Portillo Eduardo
Published: December 2024

Biomass harvesting represents one of the main bottlenecks in microalgae large-scale production. Solid–liquid separation of the biomass accounts for 30% of the total production costs, which can be reduced by the use of flocculants as a pre-concentration step in the downstream process. The natural polymer chitosan and the two chemical flocculants FeCl3 and AlCl3 were tested on freshwater Chlorella sorokiniana and two marine algae, Dunaliella tertiolecta and Tetraselmis striata. A preliminary screening at the laboratory scale was performed to detect the most suitable doses of flocculants. On the basis of these results, selected doses were tested on the pilot scale, using the flocculants for a pre-concentration step and the centrifugation as a second step to confirm the effectiveness of flocculants in a realistic operational environment. The biomass recoveries (Rpilot, %) of 100 L cultures were as follows: (1) for T. striata, Rpilot = 94.6% for 0.08 g/L AlCl3, 88.4% for 0.1 g/L FeCl3, and 68.3% for 0.04 g/L chitosan; (2) for D. tertiolecta, Rpilot = 81.7% for 0.1 g/L AlCl3, 87.9% for 0.2 g/L FeCl3, and 81.6% for 0.1 g/L chitosan; and (3) for C. sorokiniana, Rpilot = 89.6% for 0.1 g/L AlCl3, 98.6% for 0.2 g/L FeCl3, and 68.3% for 0.1 g/L chitosan. Flocculation reduced the harvesting costs by 85.9 ± 4.5% using chemical flocculants. Excesses of aluminum and iron in the biomass could be solved by decreasing the pH in the biomass combined with washing. This is the first study, to the best of our knowledge, that investigates the pilot-scale flocculation of three native Canarian microalgal strains. A pilot-scale pre-concentration step before centrifugation can improve the yield and reduce costs in the microalgae harvesting process.
Bioactive Secondary Metabolites from Harposporium anguillulae Against Meloidogyne incognita
Authors: Li Dong , Bao Ling-Feng , Lei Hong-Mei , Zhang Guang-Ke , Li Guo-Hong , Zhao Pei-Ji
Published: December 2024

Root-knot nematodes (RKNs) are pathogens that endanger a wide range of crops and cause serious global agricultural losses. In this study, we investigated metabolites of the endoparasitic fungus Harposporium anguillulae YMF1.01751, with the expectation of discovering valuable Meloidogyne incognita biocontrol compounds. Based on results obtained by a liquid chromatograph coupled to a mass spectrometer (LC-MS) of crude extracts under five culture conditions and their nematicidal activity against M. incognita, corn meal agar (CMA) medium was determined as the scale-up fermentation medium. Twelve metabolites (1–12) were isolated from the fermentation products, and compound 1 was identified to be a new cyclic tetrapeptide. The activity assay results showed that phenylacetic acid (11) had good nematicidal activity at 400 μg/mL, and the mortalities of M. incognita were 89.76% and 96.05% at 12 and 24 h, respectively, while the mortality of canthin-6-one (2) against M. incognita was 44.26% at 72 h. In addition, the results of chemotaxis activity showed that 1-(1H-indol-3-yl)ethanone (10) possessed attraction activity towards M. incognita. At the tested concentrations, cyclo-(Arg-Pro) (4) and cyclo-(Val-Ile) (7) showed an avoidant response to M. incognita. This study provides insight into the nematode-active compounds of H. anguillulae origin and offers new opportunities for the development of RKN biocontrol products.
New Insight into the Effect of Particle Surface Roughness on Flotation Efficiency: An Experimental and Theoretical Analysis
Authors: Zhang Rui , Dai Shiqi , Liu Jincheng , Yang Zhili , Nie Baisheng , Xing Yaowen , Gui Xiahui
Published: December 2024

The significance of the roughness of particles in flotation has been recognized for several decades. In this study, to investigate the effect of particle roughness, a series of rod milling with different grinding durations is performed on the quartz particles. Subsequently, the particle roundness, wetting rate, and floatability are measured. Finally, the flotation kinetic experiments are conducted based on a fixed particle size, and the interaction energy between rough particles and bubbles is calculated based on extended DLVO (E-DLVO) theory. Results show that by increasing the grinding time, the hydrophobic particle surface roughness increases, and the improved flotation rate constant increases. While the results of the E-DLVO calculation show that the interaction energy barrier between particles and bubbles decreases significantly as the roughness increases, the theoretical calculation and the experimental results confirm that an increase in the particle surface roughness decreases the interaction energy barrier between particle bubbles and increases the flotation rate.
The Weideshan Batholith and Felsic Dykes in the Eastern Jiaodong Peninsula: Is There Any Possible Relation to Gold Mineralization?
Authors: Dong Leilei , Tian Zhonghua
Published: December 2024

The genetic link between Weideshan-type rocks and gold mineralization in the Jiaodong Peninsula remains unclear. In this study, we examined the geochemical characteristics, water content, and oxidation states of Weideshan-type rocks and associated felsic dykes to assess the potential of a Weideshan-type batholith in directly contributing the fluids responsible for extensive gold mineralization. The findings reveal that the emplacement timing of Weideshan-type magmatic rocks in the Jiaodong Peninsula is slightly different from the timing of gold mineralization. Additionally, high zircon Eu/Eu* values indicate a relatively high water content within the Weideshan batholith. However, due to limited data on crystallization pressures, it remains equivocal whether water was saturated during the emplacement of the Weideshan batholith. Even if fluid saturation did occur, the magmatic oxidation states of Weideshan-type rocks are notably high (>FMQ + 1.5), which is incompatible with the reduced mineral assemblages typical of Jiaodong gold deposits. Therefore, our study suggests that the genetic link between gold mineralization and Weideshan-period granitic magmatism may be weak.
The Antioxidant and Anti-Inflammatory Activities of the Methanolic Extract, Fractions, and Isolated Compounds from Eriosema montanum Baker f. (Fabaceae)
Authors: Tabakam Gaétan Tchangou , Njoya Emmanuel Mfotie , Chukwuma Chika Ifeanyi , Mashele Samson Sitheni , Nguekeu Yves Martial Mba , Tene Mathieu , Awouafack Maurice Ducret , Makhafola Tshepiso Jan
Published: December 2024

Background: Inflammation is a natural body’s defense mechanism against harmful stimuli such as pathogens, chemicals, or irradiation. But when the inflammatory response becomes permanent, it can lead to serious health problems. In the present study, the antioxidant and anti-inflammatory potentials of the Eriosema montanum methanolic extract (EMME), as well as its isolated fractions (FA-FJ) and compounds (1–7), were evaluated by using in vitro and cellular models. Methods: The total phenolic and flavonoid contents were determined using, respectively, Folin–Ciocalteu and aluminum chloride colorimetric methods, while 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2′-diphenyl-1-picrylhy-drazyl (DPPH), and ferric ion reducing antioxidant power (FRAP) were used to determine the antioxidant activity. Thin Layer Chromatography (TLC) and column chromatography (CC) were used to isolate and purify the compounds and their elucidation using their NMR spectroscopic data. Results: EMME had moderate antioxidant and anti-inflammatory activities, while fraction FF showed much higher efficacy with IC50 values of 34.64, 30.60, 16.43, and 77.29 μg/mL against DPPH, ABTS, NO, and 15-LOX inhibitory activities, respectively. The EMME fraction was found to be very rich in flavonoids and phenolic compounds, with 82.11 mgQE/g and 86.77 mgGAE/g of dry extract, respectively. Its LC-MS profiling allowed us to identify genistin (5) as the most concentrated constituent in this plant species, which was further isolated together with six other known compounds, namely, n-hexadecane (1), heptacosanoic acid (2), tricosan-1-ol (3), lupinalbin A (4), d-pinitol (6), and stigmasterol glucoside (7). Given these compounds, genistin (5) showed moderate activity against reactive oxygen species (ROS) and NO production in LPS-stimulated RAW264.7 cells compared to EMME, which suggested a synergy of (5) with other compounds. To the best of our knowledge, compounds (1), (2), and (3) were isolated for the first time from this plant species.
Functionalities of Tremella fuciformis Polysaccharides Modified with Gallic Acid
Authors: Liu Tai-Ti , Hong Kai-Siang , Yang Tsung-Shi
Published: December 2024

This research aimed to modify polysaccharides extracted from the edible mushroom Tremella fuciformis with gallic acid (GA) and to complex them with zinc ions. The functionalities of the modified Tremella fuciformis polysaccharides (TFPs) were investigated. Regarding antioxidant activity, TFP-GA demonstrated effective scavenging activity against DPPH radicals, nitric oxide, and hydrogen peroxide. Additionally, TFP-GA exhibited superior reducing ability toward Fe3+ and enhanced chelating activity toward Fe2+ compared to unmodified TFP. Notably, the TFP-GA conjugate outperformed GA in Fe2+-chelating activity. In terms of antimicrobial activity, the TFP-GA-Zn complex showed significantly improved antimicrobial effectiveness against S. aureus and E. coli compared to TFP-GA.
The Impact of Cardiac Telerehabilitation on Health-Related Quality of Life in Patients Undergoing Percutaneous Coronary Intervention (PCI): A Systematic Review Protocol
Authors: Limonti Francesco , Gigliotti Andrea , Gravante Francesco , Ramacciati Nicola
Published: December 2024

Background: Cardiac rehabilitation (CR) is an intervention to improve health and quality of life in patients undergoing percutaneous coronary intervention (PCI). The use of digital technology for healthcare promotion, such as telemedicine, has received growing attention in recent years due to the possibility of offering remote and individualized cardiac rehabilitation to patients undergoing coronary interventions. However, the impact of cardiac telerehabilitation on health-related quality of life (HRQoL) is not fully understood. This systematic review aims to analyze through meta-analyses and synthesized comments the current knowledge on the effectiveness of cardiac telerehabilitation in improving HRQoL in patients undergoing PCI. Objectives: This manuscript presents a protocol for a systematic review to assess the effects of cardiac telerehabilitation on HRQoL in cardiac patients after PCI. Furthermore, the systematic review will explore the different modalities of remote rehabilitation documented in scientific literature. Methods: The literature review protocol was developed according to the PRISMA guidelines for systematic reviews. Search terms were structured according to the PIO (Population-Intervention-Outcome) framework. All relevant available studies will be identified using the main databases (PubMed, Scopus, CINAHL, Web of Science, and the Cochrane Library) and included in the review. Methodological quality and risk of bias will be evaluated using the Crowe Critical Appraisal Tool (CCAT). This review protocol has been registered on PROSPERO (No. CRD42024582933). Conclusions: This systematic review will comprehensively investigate the effects of cardiac telerehabilitation on QoL improvements in patients after PCI.
Role of Filter-Feeding Bivalves in the Bioaccumulation and Transmission of White Spot Syndrome Virus (WSSV) in Shrimp Aquaculture Systems
Authors: Min Joon-Gyu , Kim Young-Chul , Kim Kwang-Il
Published: December 2024

White spot syndrome virus (WSSV) poses a major risk to shrimp aquaculture, and filter-feeding bivalves on shrimp farms may contribute to its persistence and transmission. This study investigated the bioaccumulation and vector potential of WSSV in Pacific oysters (Crassostrea gigas), blue mussels (Mytilus edulis), and manila clams (Venerupis philippinarum) cohabiting with WSSV-infected shrimp. Sixty individuals of each species (average shell lengths: 11.87 cm, 6.97 cm, and 5.7 cm, respectively) cohabitated with WSSV-infected shrimp (Penaeus vannamei, average body weight: 16.4 g) for 48 h. In the experiments, bivalves accumulated WSSV particles in both the gill and digestive gland tissues, with the digestive glands exhibiting higher viral load (average viral load, 3.91 × 104 copies/mg), showing that the viral concentrations in bivalve tissues are directly influenced by seawater WSSV concentrations, reaching levels sufficient to induce infection and 100% mortality in healthy shrimp using tissue homogenates. After a 168 h release period in clean water, the WSSV levels in bivalve tissues decreased below the detection thresholds, indicating reduced transmission risk. These results highlight the role of bivalves as temporary reservoirs of WSSV in aquaculture settings, with the transmission risk dependent on the viral concentration and retention period. Our findings suggest that the management of bivalve exposure in WSSV-endemic environments could improve the biosecurity of shrimp farms.
A Zeolitic Imidazolate Framework-Based Antimicrobial Peptide Delivery System with Enhanced Anticancer Activity and Low Systemic Toxicity
Authors: Jiang Jingwen , Kaysar Kaderya , Pan Yanzhu , Xia Lijie , Li Jinyao
Published: December 2024

Background: The clinical efficacies of anticancer drugs are limited by non-selective toxic effects on healthy tissues and low bioavailability in tumor tissue. Therefore, the development of vehicles that can selectively deliver and release drugs at the tumor site is critical for further improvements in patient survival. Methods: We prepared a CEC nano-drug delivery system, CEC@ZIF-8, with a zeolite imidazole framework-8 (ZIF-8) as a carrier, which can achieve the response of folate receptor (FR). We characterized this system in terms of morphology, particle size, zeta potential, infrared (IR), x-ray diffraction (XRD), and transcriptome analysis, and examined the in vitro cytotoxicity and cellular uptake properties of CEC@ZIF-8 using cervical cancer cells. Lastly, we established a TC-1 tumor-bearing mouse model and evaluated its in vivo anti-cervical cancer activity. Results: The CEC@ZIF-8 nano-delivery system had favorable biocompatibility, heat stability, and pH responsiveness, with a CEC loading efficiency of 12%, a hydrated particle size of 174 ± 5.8 nm, a zeta potential of 20.57 mV, and slow and massive drug release in an acidic environment (pH 5.5), whereas release was 6% in a neutral environment (pH 7.4). At the same time, confocal imaging and cell viability assays demonstrated greater intracellular accumulation and more potent cytotoxicity against cancer cells compared to free CEC. The mechanism was analyzed by a series of transcriptome analyses, which revealed that CEC@ZIF-8 NPs differentially regulate the expression levels of 1057 genes in cancer cells, and indicated that the enriched pathways were mainly cell cycle and apoptosis-related pathways via the enrichment analysis of the differential genes. Flow cytometry showed that CEC@ZIF-8 NPs inhibited the growth of HeLa cells by arresting the cell cycle at the G0/G1 phase. Flow cytometry also revealed that CEC@ZIF-8 NPs induced greater apoptosis rates than CEC, while unloaded ZIF-8 had little inherent pro-apoptotic activity. Furthermore, the levels of reactive oxygen species (ROS) were also upregulated by CEC@ZIF-8 NPs while ROS inhibitors and caspase inhibitors reversed CEC@ZIF-8 NPs-induced apoptosis. Finally, CEC@ZIF-8 NPs also reduced the growth rate of xenograft tumors in mice without the systemic toxicity observed with cisplatin treatment. Conclusions: The CEC@ZIF-8 nano-drug delivery system significantly enhanced the anti-cervical cancer effect of CEC both in vivo and in vitro, providing a more promising drug delivery system for clinical applications and tumor management. At the same time, this work demonstrates the clinical potential of CEC-loaded ZIF-8 nanoparticles for the selective destruction of tumor tissues.
Nucleoside Reverse Transcriptase Inhibitor (NRTI)-Induced Neuropathy and Mitochondrial Toxicity: Limitations of the Poly-γ Hypothesis and the Potential Roles of Autophagy and Drug Transport
Authors: Haynes John , Joshi Arnav , Larue Ross C. , Eisenmann Eric D. , Govindarajan Rajgopal
Published: December 2024

Nucleoside reverse transcriptase inhibitors (NRTIs) are the backbone of highly active antiretroviral therapy (HAART)—the current standard of care for treating human immunodeficiency virus (HIV) infection. Despite their efficacy, NRTIs cause numerous treatment-limiting adverse effects, including a distinct peripheral neuropathy, called antiretroviral toxic neuropathy (ATN). ATN primarily affects the extremities with shock-like tingling pain, a pins-and-needles prickling sensation, and numbness. Despite its negative impact on patient quality of life, ATN remains poorly understood, which limits treatment options and potential interventions for people living with HIV (PLWH). Elucidating the underlying pathophysiology of NRTI-induced ATN will facilitate the development of effective treatment strategies and improved patient outcomes. In this article, we will comprehensively review ATN in the setting of NRTI treatment for HIV infection.
A QbD Approach for the Formulation and Control of Triclabendazole in Uncoated Tablets: From Polymorphs to Drug Formulation
Authors: Muzi Lucas P. , Antonio Marina , Maggio Rubén M.
Published: December 2024

Triclabendazole (TCB) is a well-established anthelmintic effective in treating fascioliasis, a neglected tropical disease. This study employs quality by design (QbD) to investigate the impact of TCB polymorphism and pharmacotechnical variables, from the development of immediate-release tablets to process optimization and green analysis. Critical process parameters (CPPs) and critical material attributes (CMAs), characterized by type of polymorph, composition of excipients (talc, lactose, cornstarch, and magnesium stearate), and compression force, were screened using a Plackett–Burman design (n = 24), identifying polymorphic purity and cornstarch as a CPP. To establish a mathematical model linking CPP to dissolution behaviour, a multiple linear regression (MLR) was applied to the training design (central composite design, n = 18). Simultaneously, a near-infrared spectroscopy coupled to partial least squares (NIR-PLSs) method was developed to analyze CPPs. An independent set of samples was prepared and analyzed using the NIR-PLSs model, and their dissolution profiles were also obtained. The PLSs model successfully predicted the CPPs in the new samples, yielding almost quantitative results (100 ± 3%), and MLR dissolution predictions mirrored the actual dissolution profiles (f2 = 85). In conclusion, the developed model could serve as a comprehensive tool for the development and control of pharmaceutical formulations, starting from the polymorphic composition and extending to achieve targeted dissolution outcomes.
Development and Validation of Kompetitive Allele-Specific Polymerase Chain Reaction Markers for Seed Protein Content in Soybean
Authors: Li Shuangzhe , Guo Chenyijun , Feng Xuezhen , Wang Jing , Pan Wenjing , Xu Chang , Wei Siming , Han Xue , Yang Mingliang , Chen Qingshan , Wang Jinxing , Hu Limin , Qi Zhaoming
Published: December 2024

Seed protein content is a critical trait in soybean breeding, as it provides a primary source of high-quality protein for both human consumption and animal feed. This study aimed to enhance molecular marker-assisted selection for high-protein soybean varieties by developing Kompetitive Allele-Specific Polymerase Chain Reaction (KASP) markers targeted at loci associated with seed protein content. Nineteen markers with high genotyping efficacy were identified through screening. Utilizing SN76 (a high-protein line) as the male parent and SN49 and DS1 (both low-protein lines) as female parents, 484 F6 generation individuals from these hybrid combinations were selected to validate the predictive accuracy of the 19 KASP markers. Notably, KASP-Pro-1, KASP-Pro-2, and KASP-Pro-3 effectively distinguished genotypes associated with high and low protein content, with prediction accuracies of 68.4%, 75.0%, and 83.3%, respectively. These results underscore the reliability and practical utility of the selected molecular markers, which are located within the genes Glyma.03G219900, Glyma.14G119000, and Glyma.17G074400, respectively. Haplotype analysis and gene pyramiding indicate that these three genes may influence seed protein content. Consequently, these KASP markers can be effectively integrated into genetic and genomic research on soybean seed protein content as well as into marker-assisted breeding.
Genetic Diversity and Identification of Vaccinium Species Through Microsatellite Analysis
Authors: Carvalho Márcia , Matos Manuela , Crespí António , Lopes Violeta R. , Carnide Valdemar
Published: December 2024

The Vaccinium genus contains about 500 species distributed worldwide but only a limited number of species have been studied for genetic diversity using molecular markers. In this study, a genetic analysis was conducted on three Vaccinium species (four cultivars of V. corymbosum, four wild populations of V. myrtillus, and two cultivars of V. ashei), for a total of 95 genotypes, using eight microsatellite (SSR) loci. A total of 57 alleles were detected. The number of alleles per locus ranged from 2 to 14, with an average of 7.25. Six unique alleles in V. corymbosum, four in V. ashei, and three in V. myrtillus were identified as being potential species markers. The dendrogram and principal coordinate analysis revealed a clear division of the three species into distinct groups, with each group further divided into sub-clusters based on the type of cultivars and population origin. The set of SSR primers used in this study demonstrated cross-species transferability, allowing their utilization in V. ashei and V. myrtillus, and can be used as a reliable tool for cultivar/population and species identification in blueberries.
The Nitrogen Preference of Cactus Pear (Opuntia ficus-indica): A Sand Culture Snapshot
Authors: Niechayev Nicholas A. , Pereira Paula N. , Cushman John C.
Published: December 2024

Cactus pear (Opuntia-ficus indica (L.) Mill.) is an important agricultural crassulacean acid metabolism (CAM) species used as a source of food, forage, fodder, and secondary products and as a biofuel feedstock. However, the preferred source of nitrogen for this species, whether it be nitrate (NO3−), ammonium (NH4+), or a combination of both, is not well understood. To investigate the nitrate and ammonium preference of cactus pear, we grew cladodes in sand culture with deionized water as a control or with a cross-factorial set of nutrient solutions of 0.0, 2.5, 5.0, and 10.0 mmol of nitrate and/or ammonium for one month. We then assessed a set of physiological parameters including cladode growth, relative water content, chlorophyll, tissue acidity, soluble sugars, starch, nitrate, ammonium, glyoxylic acid, nitrate reductase activity, and nitrogen and carbon content. We found significant differences in all measured parameters except for cladode length, relative water content, and carbon content. Cladodes provided with only deionized water produced no new cladodes and showed decreased soluble sugar content, increased starch content, and increased tissue acidity. We also determined the relative steady-state transcript abundance of genes that encode enzymes involved in N metabolism and CAM. Compared with control cladodes, nutrient-supplied cladodes generally showed increased or variable steady-state mRNA expression of selected CAM-related genes and nitrogen-metabolism-related genes. Our results suggest that O. ficus-indica prefers fertilizers containing either equal concentrations nitrate and ammonium or more nitrate than ammonium.
Genome-Wide In Silico Analysis of 1-Aminocyclopropane-1-carboxylate oxidase (ACO) Gene Family in Rice (Oryza sativa L.)
Authors: Xia Jing , Qiu Yingsheng , Li Wanli , Zhang Yingcheng , Liu Linxin , Wang Yi , Mou Wangshu , Xue Dawei
Published: December 2024

The plant hormone ethylene elicits crucial regulatory effects on plant growth, development, and stress resistance. As the enzyme that catalyzes the final step of ethylene biosynthesis, 1-Aminocyclopropane-1-carboxylic acid oxidase (ACO) plays a key role in precisely controlling ethylene production. However, the functional characterization of the ACO gene family in rice remains largely unexplored. In this study, we performed a phylogenetic analysis of seven OsACO genes (OsACO1–OsACO7), which were classified into three subfamilies (Types I, II, and III). The members within the same clades exhibited similar tertiary structures and conserved protein motifs. We conducted inter/intraspecies covariance assays of OsACOs to elucidate their evolutionary and duplication events. Numerous cis-acting elements identified in OsACO promoter regions are associated with development, hormonal stimuli, and environmental responses. The expression assay by RT-qPCR revealed that OsACO genes exhibited tissue-specific expression and were significantly altered under various abiotic stresses, indicating their potential involvement in these processes regulated at the transcriptional level. Additionally, we predicted candidate-targeting miRNAs and identified putative cysteine sites of S-nitrosylation (SNO) and S-sulfhydration (SSH) in OsACOs, providing insights into their post-transcriptional and post-translational regulatory mechanisms. These findings pave the way for the further investigation of OsACO functions and their potential applications in improving rice growth and stress resilience by modulating ethylene biosynthesis.
Bioconversion of Food and Green Waste into Valuable Compounds Using Solid-State Fermentation in Nonsterile Conditions
Authors: Bulgari Daniela , Gobbi Emanuela , Cortesi Paolo , Peron Gregorio
Published: December 2024

Agro-industrial residues have transitions from being an environmental problem to being a cost-effective source of biopolymers and value-added chemicals. However, the efficient extraction of the desired products from these residues requires pretreatments. Fungal biorefinery is a fascinating approach for the biotransformation of raw materials into multiple products in a single batch. In this study, the ability of Trichoderma asperellum R to convert fruit scrap and green waste into value-added chemicals was tested in solid-state and in nonsterile conditions. A solid-state fermentation protocol for a tray bioreactor was developed using spawn as the inoculum for nonsterile substrates. T. asperellum R drove the fermentation of both substrates, shaping the metabolites that were enriched in the secondary plant metabolites. Strain R showed cellulase activity only when inoculated on fruit scraps, resulting in increased amounts of polysaccharides in the crude extract. This extract was also enriched in vanillic acid and limonoid, which are intriguing compounds due to the increasing interest in their potential as biological nitrification inhibitors or food additives. Finally, trimethoxybenzaldehyde, an interesting chemical building block, was identified in the extracts of the Trichoderma-guided fermentation. The overall results showed that the application of T. asperellum R has potential as a driver to facilitate the extraction of bioactive substances from nonsterile recalcitrant substrates.
A Photothermal-Responsive Soft Actuator Based on Biomass Carbon Nanosheets of Synergistic Bilateral Polymers
Authors: Chen Jianze , Wei Quanzhong , Wang Honglin , Cui Wenjia , Zhang Xuewei , Wang Yuanyuan
Published: December 2024

Currently, polymer actuators capable of photothermal response are being developed to be more sensitive and repeatable. In this work, a three-layered structured soft film actuator (NA/PET/NI-3) was designed by combining poly(N-isopropylacrylamide) (PNIPAM), poly(N-(2-aminoethyl)-acrylamide) (PANGA) and poly(ethylene glycol-co-terephthalate) (PET) film. Coconut water and PEI were used to synthesize a new kind of carbon nanosheet (PEI-CCS), which, when triggered by near-infrared light, will enable photothermal bending behavior in the micrometer-scale NA/PET/NI-n film, while PET served as the supporting and heat conducting layer. This three-layered actuator utilized the synergistic effects of two kind of polymers, PNIPAM and PNAGA, on either side of PET, with the upper critical solution temperatures and lower critical solution temperatures when subjected to temperature changes. This bilateral polymer design exhibited a rapid response under near-infrared light stimulation, bending to 180° within 4 s and recovering to its original shape within 30 s. When the bending process was set to 90° as in the standard experiment, NA/PET/NI-3 responded within 2 s and recovered within 8 s. NA/PET/NI-3 also demonstrated good reversibility and repeatability, capable of undergoing reversible driving over 120 times. The design and preparation of this actuator provided new ideas for the development of polymer soft actuators.