bluuebunny/crossref_metadata_2025 huggingface dataset:
Epigenetic Regulation for Heat Stress Adaptation in Plants: New Horizons for Crop Improvement under Climate Change
Authors: Jin Qiang , Chachar Muzafaruddin , Ali Aamir , Chachar Zaid , Zhang Pingxian , Riaz Adeel , Ahmed Nazir , Chachar Sadaruddin
Published: September 2024

Global warming poses a significant threat to plant ecosystems and agricultural productivity, primarily through heat stress (HS), which disrupts photosynthesis, respiration, and overall plant metabolism. Epigenetic modifications, including DNA methylation, histone modifications, and RNA modifications, enable plants to dynamically and heritably adjust gene expression in response to environmental stressors. These mechanisms not only help plants survive immediate stress but also confer stress memory, enhancing their resilience to future HS events. This review explores the mechanisms underlying plant thermotolerance, emphasizing the critical role of epigenetic regulation in adapting to HS. It also highlights how DNA methylation modulates stress-responsive genes, histone modifications facilitate transcriptional memory, and RNA modifications influence mRNA stability and translation. Recent advancements in genome editing technologies, such as CRISPR-Cas9, have enabled precise modifications of epigenetic traits, offering new avenues for breeding climate-resilient crops. The integration of these modern tools with traditional breeding methods holds significant promise for developing crops with enhanced thermotolerance. Despite the potential, challenges such as the stability and heritability of epigenetic marks and the complex interplay between different epigenetic modifications need to be addressed. Future research should focus on elucidating these interactions and identifying reliable epigenetic markers for selection. By leveraging the insights gained from epigenetic studies, we can develop innovative breeding strategies to improve crop resilience and ensure sustainable agricultural productivity in the face of global warming. This review underscores the importance of epigenetic regulation in plant adaptation to heat stress and its potential to revolutionize crop breeding, offering a pathway to secure food production and sustainability under changing climatic conditions.
Endoplasmic Reticulum Stress Contributes to Intestinal Injury in Intrauterine Growth Restriction Newborn Piglets
Authors: Fang Tingting , Tian Gang , Chen Daiwen , He Jun , Zheng Ping , Mao Xiangbing , Yan Hui , Yu Bing
Published: September 2024

Intrauterine growth retardation (IUGR) in piglets is associated with a high rate of morbidity and mortality after birth due to gut dysfunction, and the underlying mechanisms remain poorly understood. This study selected six pairs of IUGR newborn male piglets and normal birth weight newborn piglets (Large White × Landrace) to investigate differences in intestinal structure and digestive functions, intestinal ERS and apoptosis, intestinal barrier function, and inflammatory response. The results showed that IUGR significantly reduced the jejunal villi height (p < 0.05) and the ratio of villus-height-to-crypt-depth (p = 0.05) in neonatal piglets. Additionally, the microvilli in the jejunum of IUGR neonatal piglets were shorter than those in normal-weight piglets, and swelling of the mitochondria and expansion of the endoplasmic reticulum were observed. IUGR also significantly reduced serum glucose and lactase levels (p < 0.05) while significantly increasing mRNA levels of jejunal IRE1α, EIF2α, CHOP, Bax, Caspase9, Mucin2, Claudin-1, Occludin, ZO-1, Bcl-2, IL-6, and IFN-γ (p < 0.05), as well as GRP78 protein levels in neonatal piglets (p < 0.05). These findings suggest that IUGR impairs intestinal structure and barrier function in newborn piglets by enhancing intestinal inflammatory responses, activating intestinal ERS and the signaling pathways related to the unfolded protein response, thereby inducing ERS-related apoptosis.
Nematodes Can Substitute Artemia in a Co-Feeding Regime for Pacific White Shrimp Post-Larvae Reared in a Biofloc Nursery System
Authors: Sommer Nils Phillip , Martins Mateus Aranha , Rezende Priscila Costa , Seiffert Walter Quadros , Seychelles Laurent H. , Aragão Cláudia , Vieira Felipe Boéchat
Published: September 2024

This study evaluated nematodes as an alternative to live Artemia when provided in a co-feeding regime to Pacific white shrimp (Penaeus vannamei) post-larvae (PL) reared in a biofloc nursery system. A 20-day experiment employing PL11 was performed for an evaluation of four dietary groups: control [C] (fed exclusively with a dry commercial feed); artificial Artemia [AA] (dry feed composed of extracted and processed Artemia cysts and dry commercial feed); live Artemia and dry commercial feed [LA]; and nematodes and dry commercial feed [N] (Panagrolaimus sp. dry nematodes). The diets were provided twice a day, with the remaining six feed provisions being a commercial dry feed for all experimental groups. A biofloc system was chosen as it offers a potentially sustainable approach to intensive shrimp farming. Alkalinity and pH in the [N] group were significantly higher in the last 5 days of the experiment (p < 0.05), likely causing the significantly lower nitrite levels observed in that same group (p < 0.05). Total and volatile suspended solids were significantly lower in the two live feed groups when compared with [C] and [AA] (p < 0.05). Although PL fed the dry diets exhibited higher growth rates (p < 0.05), the [N] and [LA] groups presented significantly higher final survivals (p < 0.05). No differences were found for survival after a salinity stress test (p ≥ 0.05). These results suggest that nematodes can successfully substitute Artemia in a co-feeding regime for P. vannamei PL reared in biofloc nursery systems.
Effect of Dietary Standardized Ileal Digestible Arginine to Lysine Ratio on Reproductive Performance, Plasma Biochemical Index, and Immunity of Gestating Sows
Authors: Wen Xiaolu , Jiang Zongyong , Yang Xuefen , Xiao Hao , Gao Kaiguo , Wang Li
Published: September 2024

The aim of this study was to determine the optimal SID Arg: Lys ratio for maximizing the reproductive performance, immunity and biochemical parameters of sows during gestation, the colostrum composition, and the performance of their offspring. A total of 174 multiparous sows were randomly allocated to five treatment groups varying in dietary SID Arg: Lys ratios (0.91, 1.02, 1.14, 1.25 and 1.38) through modification of the levels of Arg or alanine supplementation (the total level of nitrogen was the same in all treatments). The results showed that increasing the dietary SID Arg: Lys ratio increased the number of piglets born alive (p < 0.05, linear and quadratic). The number of stillborn piglets, the birth weight variation of born alive piglets, the birth interval (p < 0.05, linear and quadratic) and the number of mummies (p < 0.05, quadratic) reduced with increasing the dietary SID Arg: Lys ratio. Broken-line regression analysis indicated that the optimal SID Arg: Lys ratio requirement for gestating sows to maximize the number of piglets born alive was 1.25. The content of non-fat solid, total solid, protein, and energy in colostrum increased linearly and quadratically (p < 0.05) with increasing dietary SID Arg: Lys ratio. Additionally, when increasing the dietary SID Arg: Lys ratio, the concentration of IgA (p < 0.05, quadratic) and IgM (p < 0.05, linear and quadratic) of plasma increased at day 90 of gestation; IgG (p < 0.05, linear and quadratic) concentration increased at day 110 of gestation of sows. The dietary SID Arg: Lys ratio had an increasing effect (p < 0.05, linear and quadratic) on plasma insulin levels at day 90 of gestation. Furthermore, there were increases in plasma concentrations of nitric oxide and ornithine at day 110 of gestation, Arg at day 90 and 110 of gestation (p < 0.05, linear and quadratic) and total protein at day 110 of gestation (p < 0.05, linear) with increasing dietary SID Arg: Lys ratio. The results of our study indicated that increases in the dietary SID Arg: Lys ratio during gestation resulted in an increase in the number of piglets born alive, a decrease in birth intervals, and an improvement in immunity and colostrum composition. The optimal SID Arg: Lys ratio for gestating sows to maximize the number of piglets born alive was 1.25.
Infrared Thermography as a Diagnostic Tool for the Assessment of Mastitis in Dairy Ruminants
Authors: Korelidou Vera , Simitzis Panagiotis , Massouras Theofilos , Gelasakis Athanasios I.
Published: September 2024

Among the health issues of major concern in dairy ruminants, mastitis stands out as being associated with considerable losses in productivity and compromised animal health and welfare. Currently, the available methods for the early detection of mastitis are either inaccurate, requiring further validation, or expensive and labor intensive. Moreover, most of them cannot be applied at the point of care. Infrared thermography (IRT) is a rapid, non-invasive technology that can be used in situ to measure udder temperature and identify variations and inconsistencies thereof, serving as a benchmarking tool for the assessment of udders’ physiological and/or health status. Despite the numerous applications in livestock farming, IRT is still underexploited due to the lack of standardized operation procedures and significant gaps regarding the optimum settings of the thermal cameras, which are currently exploited on a case-specific basis. Therefore, the objective of this review paper was twofold: first, to provide the state of knowledge on the applications of IRT for the assessment of udder health status in dairy ruminants, and second, to summarize and discuss the major strengths and weaknesses of IRT application at the point of care, as well as future challenges and opportunities of its extensive adoption for the diagnosis of udder health status and control of mastitis at the animal and herd levels.
Current Challenges of Vaccination in Fish Health Management
Authors: Kumar Avnish , Middha Sushil Kumar , Menon Soumya Vettiyatil , Paital Biswaranjan , Gokarn Shyam , Nelli Meghana , Rajanikanth Rakshith Bangalore , Chandra Harish Mani , Mugunthan Susithra Priyadarshni , Kantwa Sanwar Mal , Usha Talambedu , Hati Akshaya Kumar , Venkatesan Divyadharshini , Rajendran Abira , Behera Tapas Ranjan , Venkatesamurthy Swarupa , Sahoo Dipak Kumar
Published: September 2024

Vaccination is an essential method of immunological preventive care required for the health management of all animals, including fish. More particularly, immunization is necessary for in-land aquaculture to manage diseases in fish broodstocks and healthy seed production. According to the latest statistics in 2020, 90.3 million tons of capture fishery production was achieved from the aquaculture sector. Out of the above, 78.8 million tons were from marine water aquaculture sectors, and 11.5 million tons were from inland water aquaculture sectors. About a 4% decline in fish production was achieved in 2020 in comparison to 2018 from inland aquaculture sectors. On the other hand, the digestive protein content, healthy fats, and nutritional values of fish products are comparatively more affordable than in other meat sources. In 2014, about 10% of aquatic cultured animals were lost (costing global annual losses > USD 10 billion) due to infectious diseases. Therefore, vaccination in fish, especially in broodstocks, is one of the essential approaches to stop such losses in the aquaculture sector. Fish vaccines consist of whole-killed pathogens, protein subunits, recombinant proteins, DNA, or live-attenuated vaccines. Challenges persist in the adaption of vaccination in the aquaculture sector, the route of administration, the use of effective adjuvants, and, most importantly, the lack of effective results. The use of autogenous vaccines; vaccination via intramuscular, intraperitoneal, or oral routes; and, most importantly, adding vaccines in feed using top dressing methods or as a constituent in fish feed are now emerging. These methods will lower the risk of using antibiotics in cultured water by reducing environmental contamination.
Therapeutic Advances in Psoriasis: From Biologics to Emerging Oral Small Molecules
Authors: Ferrara Francesco , Verduci Chiara , Laconi Emanuela , Mangione Andrea , Dondi Chiara , Del Vecchio Marta , Carlevatti Veronica , Zovi Andrea , Capuozzo Maurizio , Langella Roberto
Published: September 2024

Psoriasis is a persistent, inflammatory condition affecting millions globally, marked by excessive keratinocyte proliferation, immune cell infiltration, and widespread inflammation. Over the years, therapeutic approaches have developed significantly, shifting from conventional topical treatments and phototherapy to more sophisticated systemic interventions such as biologics and, recently, oral small-molecule drugs. This review seeks to present a comprehensive investigation of the existing psoriasis treatment options, focusing on biologic agents, oral small molecules, and emerging treatments. Several categories of biologic treatments have received regulatory approval for psoriasis, including TNF-α, IL-17, IL-12/23, and IL-23 inhibitors. Biologics have revolutionized the treatment of psoriasis. These targeted therapies offer significant improvement in disease control and quality of life, with acceptable safety profiles. However, limitations such as cost, potential immunogenicity, and administration challenges have driven the exploration of alternative treatment modalities. Oral small molecules, particularly inhibitors of Janus kinase (JAK), have emerged as options due to their convenience and efficacy. These agents represent a paradigm shift in the management of the condition, offering oral administration and targeted action on specific signaling pathways. In addition to existing therapies, the review explores emerging treatments that hold promise for the future of psoriasis care. These include innovative small-molecule inhibitors. Early-stage clinical trials suggest these agents may enhance outcomes for psoriasis patients. In conclusion, the therapeutic landscape of psoriasis is rapidly evolving, emphasizing targeted, patient-centered treatments. Ongoing research and development are expected to lead to more personalized and effective management strategies for this complex condition.
Tire Wear Emissions by Highways: Impact of Season and Surface Type
Authors: Miech Jason A. , Aker Saed , Zhang Zhaobo , Ozer Hasan , Fraser Matthew P. , Herckes Pierre
Published: September 2024

With the increasing number of electric vehicles taking to the roads, the impact of tailpipe emissions on air quality will decrease, while resuspended road dust and brake/tire wear will become more significant. This study quantified PM10 emissions from tire wear under a range of real highway conditions with measurements across different seasons and roadway surface types in Phoenix, Arizona. Tire wear was quantified in the sampled PM10 using benzothiazoles (vulcanization accelerators) as tire markers. The measured emission factors had a range of 0.005–0.22 mg km−1 veh−1 and are consistent with an earlier experimental study conducted in Phoenix. However, these results are lower than values typically found in the literature and values calculated from emissions models, such as MOVES (MOtor Vehicle Emission Simulator). We found no significant difference in tire wear PM10 emission factors for different surface types (asphalt vs. diamond grind concrete) but saw a significant decrease in the winter compared to the summer.
A Multi-Scale Analysis of the Extreme Precipitation in Southern Brazil in April/May 2024
Authors: Reboita Michelle Simões , Mattos Enrique Vieira , Capucin Bruno César , Souza Diego Oliveira de , Ferreira Glauber Willian de Souza
Published: September 2024

Since 2020, southern Brazil’s Rio Grande do Sul (RS) State has been affected by extreme precipitation episodes caused by different atmospheric systems. However, the most extreme was registered between the end of April and the beginning of May 2024. This extreme precipitation caused floods in most parts of the state, affecting 2,398,255 people and leading to 183 deaths and 27 missing persons. Due to the severity of this episode, we need to understand its drivers. In this context, the main objective of this study is a multi-scale analysis of the extreme precipitation between 26 April and 5 May, i.e., an analysis of the large-scale patterns of the atmosphere, a description of the synoptic environment, and an analysis of the mesoscale viewpoint (cloud-top features and lightning). Data from different sources (reanalysis, satellite, radar, and pluviometers) were used in this study, and different methods were applied. The National Center for Monitoring and Early Warning of Natural Disasters (CEMADEN) registered accumulated rainfall above 400 mm between 26 April and 5 May using 27 pluviometers located in the central-northern part of RS. The monthly volumes reached 667 mm and 803 mm, respectively, for April and May 2024, against a climatological average of 151 mm and 137 mm for these months. The maximum precipitation recorded was 300 mm in a single day on 30 April 2024. From a large-scale point of view, an anomalous heat source in the western Indian Ocean triggered a Rossby wave that contributed to a barotropic anticyclonic anomalous circulation over mid-southeastern Brazil. While the precipitant systems were inhibited over this region (the synoptic view), the anomalous stronger subtropical jet southward of the anticyclonic circulation caused uplift over RS State and, consequently, conditions leading to mesoscale convective system (MCS) development. In addition, the low-level jet east of the Andes transported warm and moist air to southern Brazil, which also interacted with two cold fronts that reached RS during the 10-day period, helping to establish the precipitation. Severe deep MCSs (with a cloud-top temperature lower than −80 °C) were responsible for a high lightning rate (above 10 flashes km−2 in 10 days) and accumulated precipitation (above 600 mm in 10 days), as observed by satellite measurements. This high volume of rainfall caused an increase in soil moisture, which exceeded a volume fraction of 0.55, making water infiltration into the soil difficult and, consequently, favoring flood occurrence.
Application of an Empirical Model to Improve Maximum Value Predictions in CFD-RANS: Insights from Four Scientific Domains
Authors: Efthimiou George
Published: September 2024

This study introduces an empirical model designed to predict the maximum values of time-dependent data across four turbulence-related fields: hydrogen combustion in renewable energy systems, urban microclimate effects on cultural heritage, shipping emissions, and road vehicle emissions. The model, which is based on the mean, standard deviation, and integral time scale, employs two parameters: a fixed exponent ‘ν’ (0.3) reflecting time scale sensitivity, and a variable parameter ‘b’ that accounts for application-specific uncertainties. Integrated into the Computational Fluid Dynamics (CFD) framework, specifically the Reynolds-Averaged Navier–Stokes (RANS) methodology, the model addresses the RANS approach’s limitation in predicting extreme values due to its inherent averaging process. By incorporating the empirical model, this study enhances RANS simulations’ ability to predict critical values, such as peak hydrogen concentrations and maximum urban wind speeds, which is essential for safety and reliability assessments. Validation against experimental and numerical data across the four fields demonstrates strong agreement, highlighting the model’s potential to improve CFD-RANS predictions of extreme events. This advancement offers significant implications for future CFD-RANS applications, particularly in scenarios demanding fast and reliable maximum value predictions.
ANT-Mediated Inhibition of the Permeability Transition Pore Alleviates Palmitate-Induced Mitochondrial Dysfunction and Lipotoxicity
Authors: Belosludtseva Natalia V. , Ilzorkina Anna I. , Serov Dmitriy A. , Dubinin Mikhail V. , Talanov Eugeny Yu. , Karagyaur Maxim N. , Primak Alexandra L. , Liu Jiankang , Belosludtsev Konstantin N.
Published: September 2024

Hyperlipidemia is a major risk factor for vascular lesions in diabetes mellitus and other metabolic disorders, although its basis remains poorly understood. One of the key pathogenetic events in this condition is mitochondrial dysfunction associated with the opening of the mitochondrial permeability transition (MPT) pore, a drop in the membrane potential, and ROS overproduction. Here, we investigated the effects of bongkrekic acid and carboxyatractyloside, a potent blocker and activator of the MPT pore opening, respectively, acting through direct interaction with the adenine nucleotide translocator, on the progression of mitochondrial dysfunction in mouse primary lung endothelial cells exposed to elevated levels of palmitic acid. Palmitate treatment (0.75 mM palmitate/BSA for 6 days) resulted in an 80% decrease in the viability index of endothelial cells, which was accompanied by mitochondrial depolarization, ROS hyperproduction, and increased colocalization of mitochondria with lysosomes. Bongkrekic acid (25 µM) attenuated palmitate-induced lipotoxicity and all the signs of mitochondrial damage, including increased spontaneous formation of the MPT pore. In contrast, carboxyatractyloside (10 μM) stimulated cell death and failed to prevent the progression of mitochondrial dysfunction under hyperlipidemic stress conditions. Silencing of gene expression of the predominate isoform ANT2, similar to the action of carboxyatractyloside, led to increased ROS generation and cell death under conditions of palmitate-induced lipotoxicity in a stably transfected HEK293T cell line. Altogether, these results suggest that targeted manipulation of the permeability transition pore through inhibition of ANT may represent an alternative approach to alleviate mitochondrial dysfunction and cell death in cell culture models of fatty acid overload.
Biomimetic Wings for Micro Air Vehicles
Authors: Moscato Giorgio , Romano Giovanni
Published: September 2024

In this work, micro air vehicles (MAVs) equipped with bio-inspired wings are investigated experimentally in wind tunnel. The starting point is that insects such as dragonflies, butterflies and locusts have wings with rigid tubular elements (corrugation) connected by flexible parts (profiling). So far, it is important to understand the specific aerodynamic effects of corrugation and profiling as applied to conventional wings for the optimization of low-Reynolds-number aerodynamics. The present study, in comparison to previous investigations on the topic, considers whole MAVs rather than isolated wings. A planform with a low aperture-to-chord ratio is employed in order to investigate the interaction between large tip vortices and the flow over the wing surface at large angles of incidence. Comparisons are made by measuring global aerodynamic loads using force balance, specifically drag and lift, and detailed local velocity fields over wing surfaces, by means of particle image velocimetry (PIV). This type of combined global–local investigation allows describing and relating overall MAV performance to detailed high-resolution flow fields. The results indicate that the combination of wing corrugation and profiling gives effective enhancements in performance, around 50%, in comparison to the classical flat-plate configuration. These results are particularly relevant in the framework of low-aspect-ratio MAVs, undergoing beneficial interactions between tip vortices and large-scale separation.
Experimental and Numerical Characterization of the In-Plane Shear Behavior of a Load-Bearing Hollow Clay Brick Masonry System with High Thermal Performance
Authors: Serpilli Michele , Cameli Alessandro , Stazi Francesca
Published: September 2024

Modern masonry systems are generally built with hollow clay bricks with high thermal insulating properties, fulfilling the latest sustainability and environmental criteria for constructions. Despite the growing use of sustainable masonries in seismic-prone countries, there is a notable lack of experimental and numerical data on their structural behavior under lateral in-plane loads. The present study investigates the in-plane shear behavior of load-bearing masonry walls with thin bed joints and thermal insulating hollow clay blocks. Shear-compression tests were performed on three specimens to obtain information about their shear strength, displacement capacity and failure modes. The experimental characterization was supplemented by three shear tests on triplets, along with flexural and compression tests on the mortar for the thin joints. Furthermore, two Finite Element (FE) models were built to simulate the shear-compression tests, considering different constitutive laws and brick-to-brick contact types. The numerical simulations were able to describe both the shear failure modes and the shear strength values. The results showed that the experimental shear strength was 53% higher than the one obtained through Eurocode 6. The maximum shear load was found to be up to 75% greater compared to similar masonry specimens from the literature. These findings contribute to a better understanding of the potential structural applications of sustainable hollow clay block masonry in earthquake-prone areas.
Study on the Bond Performance of Epoxy Resin Concrete with Steel Reinforcement
Authors: Chen Peiqi , Li Yueqiang , Zhou Xiaojie , Wang Hao , Li Jie
Published: September 2024

Epoxy resin concrete, characterized by its superior mechanical properties, is frequently utilized for structural reinforcement and strengthening. However, its application in structural members remains limited. In this paper, the bond–slip behavior between steel reinforcement and epoxy resin concrete was investigated using a combination of experimental research and finite element analysis, with the objective of providing data support for substantiating the expanded use of epoxy resin concrete in structural members. The research methodology included 18 center-pullout tests and 14 finite element model calculations, focusing on the effects of variables such as epoxy resin concrete strength, steel reinforcement strength, steel reinforcement diameter and protective layer thickness on bond performance. The results reveal that the bond strength between epoxy resin concrete and steel reinforcement significantly surpasses that of ordinary concrete, being approximately 3.23 times higher given the equivalent strength level of the material; the improvement in the strength of both the epoxy resin concrete and steel reinforcement are observed to marginally increase the bond stress. Conversely, an increase in the diameter of the steel reinforcement and a reduction in the thickness of the protective layer of the concrete can lead to diminished bond stress and peak slip. Particularly, when the steel reinforcement strength is below 500 MPa, it tends to reach its yield strength and may even detach during the drawing process, indicating that the yielding of the steel reinforcement occurs before the loss of bond stress. In contrast, for a steel reinforcement strength exceeding 500 MPa, yielding does not precede bond stress loss, resulting in a distinct form of failure described as scraping plough type destruction. Compared to ordinary concrete, the peak of the epoxy resin concrete and steel reinforcement bond stress–slip curve is more pointed, indicating a rapid degradation to maximum bond stress and exhibiting a brittle nature. Overall, these peaks are sharper than those of ordinary concrete, indicating a rapid decline in bond stress post-peak, reflective of its brittle characteristics.
Deconvolution-Based System Identification and Finite Element Model Calibration of the UCLA Factor Building
Authors: Wang Fei , Ma Jiemei , Kang Xiandong , Liu Qixuan , Chen Hongyu
Published: September 2024

Analysis of wave propagation within buildings in response to earthquakes enables the tracking of changes in dynamic characteristics using impulse response functions. The velocity of traveling shear waves and the intrinsic attenuation of buildings can be retrieved, providing valuable input for system identification. The Factor Building at the University of California, Los Angeles campus (henceforth referred to as the UCLA Factor Building), an instrumented 15-story steel moment frame structure, is selected for dynamic response characterization. Shear wave travel time and attenuation are computed from wave propagation using seismic interferometry applied to recorded motions, with deconvolved waves used to compute these parameters. In this study, the natural logarithm of the envelopes of waveforms deconvolved with the basement signal provided the measure of attenuation. Additionally, the waveforms deconvolved with the basement motion, indicating the building’s fundamental mode. The frequency and time decay further constrained the shear velocity and attenuation. Shear velocity was determined using arrival times measured from deconvolved waves, resulting in an average velocity of 147.1 m/s. The observed quality factor was 10.8, with a corresponding damping ratio of 5%. The shear wave velocity and damping ratio estimates derived from deconvolved waves showed consistency with those obtained from basement deconvolved waveforms. This consistency validates wave deconvolution as an effective method for isolating building response from excitation and ground coupling. By incorporating the resonant frequencies and damping ratios derived from previous analyses into a refined element model, this study underscores the potential of wave deconvolution for extracting building dynamic characteristics, thereby enhancing our understanding of their responses to earthquakes.
Experimental and Numerical Study on the Performance of Steel–Coarse Aggregate Reactive Powder Concrete Composite Beams with Uplift-Restricted and Slip-Permitted Connectors under Negative Bending Moment
Authors: Zhong Xuan-Yang , Zhuang Liang-Dong , Ding Ran , Tao Mu-Xuan
Published: September 2024

An innovative form of steel–concrete composite beam, the steel–coarse aggregate reactive powder concrete (CA-RPC) composite beam with uplift-restricted and slip-permitted (URSP) connectors, is introduced in this paper. The aim is to enhance the cracking resistance under negative bending moments, which is a difficult problem for traditional composite beams, and to make the cost lower than using ordinary reactive powder concrete (RPC). An experimental investigation of the behavior of six specimens of simply supported steel–CA-RPC composite beams with URSP connectors under negative bending moments is presented in this paper. The test results validated that the cracking load of steel–CA-RPC composite beams could be approximately three times that of the ordinary steel–concrete composite beams while the bearing capacity and stiffness are almost the same. A numerical model, using the concrete damaged plasticity (CDP) model to simulate the behavior of the CA-RPC material, was proposed and successfully calculated the overall load–displacement relationship of the composite beams with sufficient accuracy compared with the experimental results, and the distribution of cracks and the failure mode of the beams could also be captured by this model. Furthermore, a parametric analysis was carried out to find out how the application of prestress, CA-RPC, and URSP connectors could affect the cracking resistance of the composite beams, and the results indicated that using CA-RPC and prestress made the main contributions and that the usage of URSP could boost the effect of the other two factors. The plastic resistance moment of the beams was also compared with the calculation results using the methods introduced in Eurocode 4, and it was proved that the calculation results were lower than the experimental results by approximately 10%, which meant that the method was reliable for this kind of composite beam.
Investigating the Satisfaction of Residents in the Historic Center of Macau and the Characteristics of the Townscape: A Decision Tree Approach to Machine Learning
Authors: Yang Shuai , Chen Yile , Huang Yuhao , Zheng Liang , Huang Yue
Published: September 2024

The historic city of Macau is China’s 31st world heritage site, and its residents have actively contributed to preserving its heritage and will continue to reside there for the foreseeable future. Residents’ satisfaction with the current urban environment is closely related to the landscape characteristics of the towns surrounding the historic center of Macau. This study aims to analyze the relationship between landscape characteristics and residents’ satisfaction, determine the key factors affecting their satisfaction and how they are combined, and provide a scientific basis for urban planning. This study used a decision tree machine learning model to analyze 524 questionnaire survey responses that addressed five aspects of the historic town’s landscape: the architectural, Largo Square, street, mountain and sea, and commercial landscapes. The data-driven approach helped find the best decision path. The results indicate that (1) the layout of Largo Square, the commercial colors and materials, the location of the former humanities and religion center, and the commercial signage system are the primary factors influencing residents’ satisfaction. (2) Incorporating decision tree parameters with information entropy as the splitting criterion and a minimum sample split number of two (with no maximum depth) led to the best performance when investigating residents’ satisfaction with Macau’s historic town landscape characteristics. (3) A reasonable layout for Largo Square (satisfaction > 3.50), prominent and harmonious commercial colors and materials (satisfaction > 3.50), rich cultural and religious elements (satisfaction > 4.50), and an excellent commercial signage system (satisfaction > 4.00) can significantly improve residents’ satisfaction. This provides important empirical support and a reference for urban planning and landscape design in Macau and other historical and cultural cities.
A Study on the Bearing Performance of an RC Axial Compression Shear Wall Strengthened by a Replacement Method Using Local Reinforcement with an Unsupported Roof
Authors: Liu Yuanwen , Deng Jie , Jia Yigang , Wu Guangyu , Ke Naiwen , Wei Xianglan
Published: September 2024

When compared with conventional replacement reinforcement methods, the method of replacement using a local reinforcement with an unsupported roof has the advantages of shortening the reinforcement cycle and reducing material loss, and many scholars have carried out useful explorations thereof. At present, the formula for the bearing capacity of reinforcement by replacing concrete in the Code does not consider the effect of stress hysteresis on the parts of the reinforcement; so when the initial stress level is greater than 0.4, the Code’s strength utilization coefficient of 0.8 for the new concrete in the replaced area is on the side of insecurity. In this study, we are trying to improve and supplement the formula in the Code through the following work. Firstly, 18 groups of shear wall models were constructed using a VFEAP finite element analysis program to analyze the bearing performances of the shear walls after the replacement. The results showed that the replacement concrete strength, the initial stress level and the size of the replaced area had a significant influence on the bearing capacity of the shear wall after the replacement. Secondly, utilizing the replacement concrete strength, the initial stress level and the size of the replacement area as key parameters, then introducing the strength improvement coefficient considering the constraints of the stirrups, the modified strength utilization coefficient of new concrete in the replaced area was formulated. Finally, based on the modified strength utilization coefficient, the replacement bearing capacity formulas for the one-batch, two-batch, and three-batch replacements were derived, and an N-batch replacement bearing capacity formula was regressed and fitted on the basis of these equations, which are less discrete and more secure than the Code’s formula.
Immune-Cell-Derived Exosomes as a Potential Novel Tool to Investigate Immune Responsiveness in SCLC Patients: A Proof-of-Concept Study
Authors: Amato Luisa , De Rosa Caterina , De Rosa Viviana , Heydari Sheikhhossein Hamid , Ariano Annalisa , Franco Paola , Nele Valeria , Capaldo Sara , Di Guida Gaetano , Sepe Filippo , Di Liello Alessandra , De Rosa Giuseppe , Tuccillo Concetta , Gambardella Antonio , Ciardiello Fortunato , Morgillo Floriana , Tirino Virginia , Della Corte Carminia Maria , Iommelli Francesca , Vicidomini Giovanni
Published: September 2024

Small cell lung cancer (SCLC) is a highly invasive and rapidly proliferating lung tumor subtype. Most patients respond well to a combination of platinum-based chemotherapy and PD-1/PDL-1 inhibitors. Unfortunately, not all patients benefit from this treatment regimen, and few alternative therapies are available. In this scenario, the identification of new biomarkers and differential therapeutic strategies to improve tumor response becomes urgent. Here, we investigated the role of exosomes (EXs) released from the peripheral blood mononuclear cells (PBMCs) of SCLC patients in mediating the functional crosstalk between the immune system and tumors in response to treatments. In this study, we showed that PBMC-EXs from SCLC patients with different responses to chemoimmunotherapy showed different levels of immune (STING and MAVS) and EMT (Snail and c-Myc) markers. We demonstrated that PBMC-EXs derived from best responder (BR) patients were able to induce a significant increase in apoptosis in SCLC cell lines in vitro compared to PBMC-EXs derived from non-responder (NR) SCLC patients. PBMC-EXs were able to affect cell viability and modulate apoptotic markers, DNA damage and the replication stress pathway, as well as the occurrence of EMT. Our work provides proof of concept that PBMC-EXs can be used as a tool to study the crosstalk between cancer cells and immune cells and that PBMC-EXs exhibit an in vitro ability to promote cancer cell death and reduce tumor aggressiveness.
Non-Invasive Endometrial Cancer Screening through Urinary Fluorescent Metabolome Profile Monitoring and Machine Learning Algorithms
Authors: Švecová Monika , Dubayová Katarína , Birková Anna , Urdzík Peter , Mareková Mária
Published: September 2024

Endometrial cancer is becoming increasingly common, highlighting the need for improved diagnostic methods that are both effective and non-invasive. This study investigates the use of urinary fluorescence spectroscopy as a potential diagnostic tool for endometrial cancer. Urine samples were collected from endometrial cancer patients (n = 77), patients with benign uterine tumors (n = 23), and control gynecological patients attending regular checkups or follow-ups (n = 96). These samples were analyzed using synchronous fluorescence spectroscopy to measure the total fluorescent metabolome profile, and specific fluorescence ratios were created to differentiate between control, benign, and malignant samples. These spectral markers demonstrated potential clinical applicability with AUC as high as 80%. Partial Least Squares Discriminant Analysis (PLS-DA) was employed to reduce data dimensionality and enhance class separation. Additionally, machine learning models, including Random Forest (RF), Logistic Regression (LR), Support Vector Machine (SVM), and Stochastic Gradient Descent (SGD), were utilized to distinguish between controls and endometrial cancer patients. PLS-DA achieved an overall accuracy of 79% and an AUC of 90%. These promising results indicate that urinary fluorescence spectroscopy, combined with advanced machine learning models, has the potential to revolutionize endometrial cancer diagnostics, offering a rapid, accurate, and non-invasive alternative to current methods.
Epidemiology, Diagnostics, and Therapy of Oral Cancer—Update Review
Authors: Kijowska Julia , Grzegorczyk Julia , Gliwa Katarzyna , Jędras Aleksandra , Sitarz Monika
Published: September 2024

Oral cavity and lip cancers are the 16th most common cancer in the world. It is widely known that a lack of public knowledge about precancerous lesions, oral cancer symptoms, and risk factors leads to diagnostic delay and therefore a lower survival rate. Risk factors, which include drinking alcohol, smoking, HPV infection, a pro-inflammatory factor-rich diet, and poor oral hygiene, must be known and avoided by the general population. Regular clinical oral examinations should be enriched in an oral cancer search protocol for the most common symptoms, which are summarized in this review. Moreover, new diagnostic methods, some of which are already available (vital tissue staining, optical imaging, oral cytology, salivary biomarkers, artificial intelligence, colposcopy, and spectroscopy), and newly researched techniques increase the likelihood of stopping the pathological process at a precancerous stage. Well-established oral cancer treatments (surgery, radiotherapy, chemotherapy, and immunotherapy) are continuously being developed using novel technologies, increasing their success rate. Additionally, new techniques are being researched. This review presents a novel glance at oral cancer—its current classification and epidemiology—and will provide new insights into the development of new diagnostic methods and therapies.
Unplanned Excision of Synovial Sarcoma: Factors Associated with Recurrence and Survival
Authors: Broida Samuel E. , Arguello Alexandra M. , Sullivan Mikaela H. , Robinson Steven I. , Okuno Scott H. , Siontis Brittany L. , Ho Thanh P. , Rose Peter S. , Xu-Welliver Meng , Houdek Matthew T.
Published: September 2024

Background: Synovial sarcoma is rare and may present as a small, slow-growing mass. These tumors are often mistaken as benign and are therefore prone to unplanned and/or non-oncologic excision. We sought to identify the rate of unplanned excision of synovial sarcoma and risk factors for recurrence and survival among this cohort. Methods: The medical records of 246 patients evaluated at a single institution for synovial sarcoma between 1997 and 2022 were retrospectively reviewed. Of these, 87 (35%) underwent unplanned, non-oncologic excision. The mean age of the cohort was 49 years. Primary tumors were located in the extremity (n = 63), abdomen (n = 6), thorax (n = 7), head/neck (n = 8), and paraspinal region (n = 3). The median maximum pre-treatment dimension of the primary tumor was 4.8 cm (IQR 7–2.4). Seventy-seven (86%) patients underwent re-excision of the tumor bed, 39 (45%) received chemotherapy, and 63 (72%) received radiation therapy. Results: Among patients who underwent unplanned excision, local recurrence-free survival (LRFS) was 98% at 1 year and 82% at 5 years. Metastasis-free survival (MFS) was 91% at 1 year and 72% at 5 years. Disease-specific survival (DSS) was 98% at 1 year and 72% at 5 years. When adjusting for tumor size, tumors which underwent unplanned excision did not have worse recurrence or survival compared to those which had planned excision (p > 0.10). Size > 5 cm, monophasic subtype, and axial location were associated with increased risk of disease recurrence. Forty-six patients had residual tumor following re-excision, which was associated with worse MFS (HR 8.17, 95% CI [1.89, 35.2], p < 0.01) and DSS (HR 7.66, 95% CI [1.76, 33.4], p < 0.01). Patients who received radiotherapy had improved MFS (HR 6.4, 95% CI [1.42, 29.0], p = 0.02) and DSS (HR 5.86, 95% CI [1.27, 26.9], p = 0.02). Conclusions: One-third of patients presenting with synovial sarcoma were diagnosed after unplanned, non-oncologic excision. Patients with large, axial tumors had worse survival. Approximately half of patients who underwent unplanned excision had no residual tumor after pre-operative radiation. The use of radiation was associated with decreased rates of recurrence and improved disease-specific survival. Our results suggest that margin-negative re-resection and radiotherapy should be considered when feasible following unplanned excision of synovial sarcoma.
Gastric Cancer: An Up-to-Date Review with New Insights into Early-Onset Gastric Cancer
Authors: Mazurek Marek , Szewc Monika , Sitarz Monika Z. , Dudzińska Ewa , Sitarz Robert
Published: September 2024

Gastric cancer (GC) is the fifth most frequently diagnosed cancer and the fifth most common cause of cancer death in the world. Regarding the age at which the diagnosis was made, GC is divided into early-onset gastric cancer (EOGC—up to 45 years of age) and conventional GC (older than 45). EOGC constitutes approximately 10% of all GCs. Numerous reports indicate that EOGC is more aggressive than conventional GC and is often discovered at an advanced tumor stage, which has an impact on the five-year survival rate. The median survival rate for advanced-stage GC is very poor, amounting to less than 12 months. Risk factors for GC include family history, alcohol consumption, smoking, Helicobacter pylori, and Epstein–Barr virus infection. It has been shown that a proper diet and lifestyle can play a preventive role in GC. However, research indicates that risk factors for conventional GC are less correlated with EOGC. In addition, the unclear etiology of EOGC and the late diagnosis of this disease limit the possibilities of effective treatment. Genetic factors are considered a likely cause of EOGC, as young patients are less exposed to environmental carcinogens. Research characterizing GC in young patients is scarce. This comprehensive study presents all aspects: epidemiology, risk factors, new treatment strategies, and future directions.
Exploring Tumor Heterogeneity: Radiogenomic Assessment of ADFP in Low WHO/ISUP Grade Clear Cell Renal Cell Carcinoma
Authors: Greco Federico , Panunzio Andrea , D’Andrea Valerio , Vescovo Mariavittoria , Tafuri Alessandro , Carotti Simone , Beomonte Zobel Bruno , Mallio Carlo Augusto
Published: September 2024

This study aimed to investigate the association between metabolic lipid computed tomography (CT) features and adipose differentiation-related protein (ADFP) expression in clear cell renal cell carcinoma (ccRCC), providing insights into non-invasive methods for assessing ADFP expression and tumor characteristics. This study utilized data from The Cancer Genome Atlas and the Cancer Imaging Archive to analyze genetic alterations and imaging characteristics in ccRCC patients. Tumoral Hounsfield units (HU) analysis and quantification of abdominal adipose tissue compartments were performed using CT images. Statistical analyses were conducted to compare tumoral HU values according to ADFP gene expression and World Health Organization/International Society of Urological Pathology (WHO/ISUP) tumor grade, as well as to explore correlations between tumoral HU values and adipose tissue quantification. Among the 174 identified patients, those with ADFP gene expression showed significantly lower minimum tumoral HU values in low-grade cancers compared to high-grade cancers. Similarly, patients with low-grade cancers expressing ADFP exhibited lower minimum tumoral HU values compared to those without ADFP expression. Negative correlations were observed between minimum tumoral HU values and visceral adipose tissue, subcutaneous adipose tissue, and total adipose tissue in both ccRCC patients with and without ADFP expression. This study reveals a significant association between metabolic lipid CT features and ADFP expression in ccRCC patients. Lower minimum tumoral HU values, suggestive of higher intracellular lipid accumulation, were observed in tumors with low WHO/ISUP grade and ADFP expression.