bluuebunny/crossref_metadata_2025 huggingface dataset:
To Kill a Mockingbird by Harper Lee
Authors: Armstrong Jean
Published: October 2015

Nelle Harper Lee was born on 26 April 1926 in Monroeville, Alabama, which is most certainly the Maycomb of the novel. She was the youngest daughter of a lawyer and began to write early in childhood while she was attending local schools. Her satirical presentation of education in the novel suggests that she found much to criticise there.
Austria-Hungary and the Origins of the First World War
Authors: Williamson , Jr Samuel R.
Published: October 2015

A major re-examination of Habsburg decision-making from 1912 to July 1914, the study argues that Austria-Hungary and not Germany made the crucial decisions for war in the summer of 1914. Based on extensive new archival research, the book traces the gradual militarization of Austro-Hungarian foreign policy during the Balkan Wars. The disasters of those wars and the death of the Archduke Franz Ferdinand, the heir-apparent and a force for peace in the monarchy, convinced the Habsburg elite that only a war against Serbia would end the South Slav threat to the monarchy's existence. Williamson also describes Russia's assertive foreign policy after 1912 and stresses the unique linkages of domestic and foreign policy in almost every issue faced by Habsburg statesmen.
Quality of Life following ACL Reconstruction: Baseline Predictors of Patient-Reported Outcomes
Authors: Johnson Christine C. , Garcia Grant H. , Garner Matthew R. , Marx Robert G.
Published: October 2015

The study by Dunn et al., “Baseline Predictors of Health-Related Quality of Life after Anterior Cruciate Ligament Reconstruction: A longitudinal analysis of a multicenter cohort at two and six years,” evaluates patient factors that are predictive of outcomes following anterior cruciate ligament reconstruction. The current review critically analyzes the findings of this study in light of the current body of literature on the subject and assesses its contribution to the development of evidence-based guidelines. The authors’ primary endpoint, the Short Form-36 (SF-36), is used ubiquitously in health care research and allows their results to be compared across different disease states and studies. Despite its widespread use, the SF-36 has been shown to be sensitive to outcomes following anterior cruciate ligament reconstruction. The authors’ use of generic health-related quality of life outcome as a primary endpoint represents an important contribution to the field, and their findings allow for improved preoperative counseling by identifying baseline patient factors that predict outcomes following anterior cruciate ligament reconstruction. Furthermore, by deriving utilities from SF-36 scores, the authors are able to assess the value of anterior cruciate ligament reconstruction as compared to other medical and surgical procedures.
Assessment of Osteonecrosis in the Presence of Instrumentation for Femoral Neck Fracture Using Contrast-Enhanced MAVRIC Sequence
Authors: Farshad-Amacker Nadja A. , Koff Matthew F. , Dyke Jonathan P. , Lazaro Lionel E. , Shah Parina , Lorich Dean G. , Potter Hollis G.
Published: October 2015

Background

Evaluating postoperative femoral neck facture (FNF) with metal fixation hardware is commonly performed using radiographs. MRI has greater sensitivity and specificity to evaluate osteonecrosis (ON) but is often challenging due to the image distortion caused by metallic hardware.

Questions/Purposes

The aim of this study is to compare fast spin-echo (FSE) and multi-acquisition variable-resonance image combination (MAVRIC) sequences in assessing ON following metallic fixation of FNF and determining feasibility of semi-quantitative perfusion using MAVRIC.

Methods

Radiography and MRI were performed at 3 and 12 months postoperatively, using FSE and pre- and post-gadolinium contrast MAVRIC sequences in 21 FNF patients. The presence and volume of ON were recorded. Signal intensity (SI) enhancement was measured on the MAVRIC sequences within the center and rim of ON; with the ilium and femoral diaphysis as controls. The detection rate of ON between MAVRIC and FSE images was evaluated as the difference of percent enhancement across the defined regions of interest.

Results

ON was detected in 0% of radiographs, in 67% of FSE, and in 76% of MAVRIC images at 3 months follow-up, with similar results at 12 months. MAVRIC images had larger ON volume than FSE images at both time points. A significant percentage SI enhancement was only detected in the ON rim.

Conclusion

Radiographs could not detect ON following metallic fixation of FNF. MAVRIC is more sensitive than FSE for determining the volume of ON. SI measurements using MAVRIC may provide an indirect assessment of perfusion.
Immobilization of Lipid Substrates: Application on Phospholipase A2 Determination
Authors: Karkabounas Athanassios , Georgiadou Dimitra G. , Argitis Panagiotis , Psycharis Vassilios , Nakos George , Kosmas Agni M. , Lekka Marilena E.
Published: October 2015

The purpose of the study was to assess a fluorimetric assay for the determination of total phospholipase A2 (PLA2) activity in biological samples introducing the innovation of immobilized substrates on crosslinked polymeric membranes. The immobilized C12‐NBD‐PtdCho, a fluorescent analogue of phosphatidylcholine, exhibited excellent stability for 3 months at 4 °C and was not desorbed in the aqueous reaction mixture during analysis. The limit of detection was 0.5 pmol FA (0.2 pg) and the linear part of the response curve extended from 1 up to 190 nmol FA/h/mL sample. The intra‐ and inter‐day relative standard deviations (%RSD), were ≤6 and ≤9 %, respectively. Statistical comparison with other fluorescent methods showed excellent correlation and agreement. Semiempirical calculations showed a fair amount of electrostatic interaction between the NBD‐labeled substrate and the crosslinked polyvinyl alcohol with the styryl pyridinium residues (PVA‐SbQ) material, from the plane of which, the sn‐2 acyl chain of the phospholipid stands out and is accessible by PLA2. Atomic Force Microscopy revealed morphological alterations of the immobilized substrate after the reaction with PLA2. Mass spectrometry showed that only C12‐NBD‐FA, the PLA2 hydrolysis product, was detected in the reaction mixture, indicating that PLA2 recognizes PVA‐SbQ/C12‐NBD‐PtdCho as a surface to perform catalysis.
Competency-based medical education and scholarship: Creating an active academic culture during residency
Authors: Bourgeois James A. , Ana Hategan Ana , Azzam Amin
Published: October 2015

The competency-based medical education movement has been adopted in several medical education systems across the world. This has the potential to result in a more active involvement of residents in the educational process, inasmuch as scholarship is regarded as a major area of competency. Substantial scholarly activities are well within the reach of motivated residents, especially when faculty members provide sufficient mentoring. These academically empowered residents have the advantage of early experience in the areas of scholarly discovery, integration, application, and teaching. Herein, the authors review the importance of instituting the germinal stages of scholarly productivity in the creation of an active scholarly culture during residency. Clear and consistent institutional and departmental strategies to promote scholarly development during residency are highly encouraged.
Evaluation of the effects of riluzole on adult spinal cord‐derived neural stem/progenitor cells in vitro and in vivo
Authors: Hachem Laureen D. , Mothe Andrea J. , Tator Charles H.
Published: October 2015

Riluzole, a sodium/glutamate antagonist, has shown significant neuroprotective effects in experimental models of spinal cord injury (SCI) and is currently under clinical trial for patients with SCI. However, the effect of riluzole on adult spinal cord‐derived NSPCs remains unknown. In this study, we examined the effects of riluzole on NSPC survival both in vitro and in vivo. NSPCs harvested from the adult rat spinal cord were exposed to riluzole (1–30 μM) either alone or in combination with hydrogen peroxide or glutamate in vitro. Measures of intracellular reactive oxygen species (ROS), cell viability and proliferation were assessed. To examine the effects of riluzole on transplanted NSPCs in vivo, a rodent clip compression model of SCI was used. One week after injury, NSPCs were transplanted into the spinal cord and rats received either riluzole or vehicle treatment for two weeks (similar to the clinically accepted dosing regimen) at which time cords were processed for analysis. Exposure to riluzole (≥10 μM) for more than 48 h in vitro reduced NSPC viability. Riluzole treatment (1–10 μM) did not significantly affect intracellular ROS levels or cell viability in the setting of in vitro oxidative stress. While glutamate (500 μM) exposure for 96 h significantly increased adult NSPC proliferation and survival, this response was not blocked by concurrent treatment with riluzole (1–10 μM) thus supporting the notion that the known anti‐glutamatergic properties of riluzole are not mediated through direct inhibition of glutamate receptors. Furthermore, riluzole treatment did not impair the survival of transplanted NSPCs in a rodent model of SCI. These results suggest that although NSPCs may have a narrow tolerance to riluzole treatment in vitro, riluzole does not impair NSPC survival at doses that would be used clinically.
Dynasties
Authors: Duindam Jeroen
Published: October 2015

For thousands of years, societies have fallen under the reign of a single leader, ruling as chief, king, or emperor. In this fascinating global history of medieval and early modern dynastic power, Jeroen Duindam charts the rise and fall of dynasties, the rituals of rulership, and the contested presence of women on the throne. From European, African, Mughal, Ming-Qing and Safavid dynasties to the Ottoman Empire, Tokugawa Japan and Chosŏn Korea, he reveals the tension between the ideals of kingship and the lives of actual rulers, the rich variety of arrangements for succession, the households or courts which catered to rulers' daily needs, and the relationship between the court and the territories under its control. The book integrates numerous African examples, sets dynasties within longer-term developments such as the rise of the state, and examines whether the tensions inherent in dynastic power led inexorably to cycles of ascent and decline.
Jewish Law and Contemporary Issues
Authors: Bleich J. David , Jacobson Arthur J.
Published: October 2015

Organized as a series of authoritative discussions, this book presents the application of Jewish law - or Halakhah - to contemporary social and political issues. Beginning with the principle of divine revelation, it describes the contents and canons of interpretation of Jewish law. Though divinely received, the law must still be interpreted and 'completed' by human minds, often leading to the conundrum of divergent but equally authentic interpretations. Examining topics from divorce to war and from rabbinic confidentiality to cloning, this book carefully delineates the issues presented in each case, showing the various positions taken by rabbinic scholars, clarifying areas of divergence, and analyzing reasons for disagreement. Written by widely recognized scholars of both Jewish and secular law, this book will be an invaluable source for all who seek authoritative guidance in understanding traditional Jewish law and practice.
Bayesian Evolutionary Analysis with BEAST
Authors: Drummond Alexei J. , Bouckaert Remco R.
Published: October 2015

What are the models used in phylogenetic analysis and what exactly is involved in Bayesian evolutionary analysis using Markov chain Monte Carlo (MCMC) methods? How can you choose and apply these models, which parameterisations and priors make sense, and how can you diagnose Bayesian MCMC when things go wrong? These are just a few of the questions answered in this comprehensive overview of Bayesian approaches to phylogenetics. This practical guide:
• Addresses the theoretical aspects of the field
• Advises on how to prepare and perform phylogenetic analysis
• Helps with interpreting analyses and visualisation of phylogenies
• Describes the software architecture
• Helps developing BEAST 2.2 extensions to allow these models to be extended further.
With an accompanying website providing example files and tutorials (http://beast2.org/), this one-stop reference to applying the latest phylogenetic models in BEAST 2 will provide essential guidance for all users – from those using phylogenetic tools, to computational biologists and Bayesian statisticians.
Foreign Opera at the London Playhouses
Authors: Fuhrmann Christina
Published: October 2015

In the early nineteenth century over forty operas by foreign composers, including Mozart, Rossini, Weber and Bellini, were adapted for London playhouses, often appearing in drastically altered form. Such changes have been denigrated as 'mutilations'. The operas were translated into English, fitted with spoken dialogue, divested of much of their music, augmented with interpolations and frequently set to altered libretti. By the end of the period, the radical changes of earlier adaptations gave way to more faithful versions. In the first comprehensive study of these adaptations, Christina Fuhrmann shows how integral they are to our understanding of early nineteenth-century opera and the transformation of London's theatrical and musical life. This book reveals how these operas accelerated repertoire shifts in the London theatrical world, fostered significant changes in musical taste, revealed the ambiguities and inadequacies of copyright law and sparked intense debate about fidelity to the original work.
The Poems of Catullus
Authors: Catullus
Published: October 2015

The Roman poet Catullus is one of the most popular and frequently studied ancient authors. His poems were written just over two thousand years ago during the chaotic but culturally vibrant final decades of the Republic and deal with themes of passion and grief, friendship and enmity, politics, literature and myth. This new translation, the product of a collaborative effort between a classicist and a poet, allows modern readers to experience his poems rather as his ancient Roman audience did. The poems are presented as contemporary and concise with a new energy and pace that both enhance Catullus' appeal for non-specialists and challenge specialists to consider his work from a fresh perspective. Extensive notes are provided, as well as an introduction which takes account of modern poetics and popular culture. The translation will appeal not only to classicists but also to lovers of literature in general and poetry in particular.
Climate Change, Capitalism, and Corporations
Authors: Wright Christopher , Nyberg Daniel
Published: October 2015

Climate change is one of the greatest threats facing humanity, a definitive manifestation of the well-worn links between progress and devastation. This book explores the complex relationship that the corporate world has with climate change and examines the central role of corporations in shaping political and social responses to the climate crisis. The principal message of the book is that despite the need for dramatic economic and political change, corporate capitalism continues to rely on the maintenance of 'business as usual'. The authors explore the different processes through which corporations engage with climate change. Key discussion points include climate change as business risk; corporate climate politics; the role of justification and compromise; and managerial identity and emotional reactions to climate change. Written for researchers and graduate students, this book moves beyond descriptive and normative approaches to provide a sociologically and critically informed theory of corporate responses to climate change.
Nature, Culture, and Society
Authors: Palsson Gisli
Published: October 2015

Life is currently one of the most active zones of politics and economic production, as biological material is increasingly the subject of engineering, banking, reproduction, and exchange. These developments represent some of the most challenging issues facing humanity in the twenty-first century and call for new forms of engagement - and new anthropologies of life. Reflecting upon the changing human condition, Palsson addresses various conflated zones of life at particular times and scales, from the genome to the human body and the global environment. Using a 'biosocial' perspective, he argues, will help us to capture the hybrid nature of life, enhancing our sensitivity to differences and similarities in hierarchies, the reproduction of bio-objects and the exchange between humans, other species, and the environment. Engaging with topical issues on the public agenda, from personal genomics to human-animal relations to the global environment, the book sets out a compelling case for meaningful change.
Paper Tiger
Authors: Mathur Nayanika
Published: October 2015

A big cat overthrows the Indian state and establishes a reign of terror over the residents of a Himalayan town. A welfare legislation aimed at providing employment and commanding a huge budget becomes 'unimplementable' in a region bedeviled by high levels of poverty and unemployment. Paper Tiger provides a lively ethnographic account of how such seemingly bizarre scenarios come to be in contemporary India. Based on eighteen months of intensive fieldwork, this book presents a unique explanation for why and how progressive laws can do what they do and not, ever-so-often, what they are supposed to do. It reveals the double-edged effects of the reforms that have been ushered in by the post-liberalization Indian state, particularly the effort to render itself more transparent and accountable. Through a meticulous detailing of everyday bureaucratic life on the Himalayan borderland, Paper Tiger makes an argument for shifting the very frames of thought through which we apprehend the workings of the developmental Indian state.
Taxation, Responsiveness and Accountability in Sub-Saharan Africa
Authors: Prichard Wilson
Published: October 2015

It is increasingly argued that bargaining between citizens and governments over tax collection can provide a foundation for the development of responsive and accountable governance in developing countries. However, while intuitively attractive, surprisingly little research has captured the reality and complexity of this relationship in practice. This book provides the most complete treatment of the connections between taxation and accountability in developing countries, providing both new evidence and an invaluable starting point for future research. Drawing on cross-country econometric evidence and detailed case studies from Ghana, Kenya and Ethiopia, Wilson Prichard shows that reliance on taxation has, in fact, increased responsiveness and accountability by expanding the political power wielded by taxpayers. Critically, however, processes of tax bargaining have been highly varied, frequently long term and contextually contingent. Capturing this diversity provides novel insight into politics in developing countries and how tax reform can be designed to encourage broader governance gains.
Pindar and the Emergence of Literature
Authors: Maslov Boris
Published: October 2015

Pindar and the Emergence of Literature places Pindar in the context of the evolution of Archaic Greek poetics. While presenting an in-depth introduction to diverse aspects of Pindar's art (authorial metapoetics, imagery, genre hybridization, religion, social context, and dialect), it seeks to establish a middle ground between cultural contextualism and literary history, paying attention both to poetry's historical milieu and its uncanny capacity to endure in time. With that methodological objective, the book marshals a new version of historical poetics, drawing both on theorists usually associated with this approach, such as Alexander Veselovsky, Mikhail Bakhtin, and Olga Freidenberg, and on T. S. Eliot, Hans Blumenberg, Fredric Jameson, and Stephen Greenblatt. The ultimate literary-historical problem posed by Pindar's poetics, which this book sets out to solve, is the transformation of pre-literary structures rooted in folk communal art into elements that still inform our notion of literature.
Socrates and Self-Knowledge
Authors: Moore Christopher
Published: October 2015

In this book, the first systematic study of Socrates's reflections on self-knowledge, Christopher Moore examines the ancient precept 'Know yourself' and, drawing on Plato, Aristophanes, Xenophon, and others, reconstructs and reassesses the arguments about self-examination, personal ideals, and moral maturity at the heart of the Socratic project. What has been thought to be a purely epistemological or metaphysical inquiry turns out to be deeply ethical, intellectual, and social. Knowing yourself is more than attending to your beliefs, discerning the structure of your soul, or recognizing your ignorance - it is constituting yourself as a self who can be guided by knowledge toward the good life. This is neither a wholly introspective nor a completely isolated pursuit: we know and constitute ourselves best through dialogue with friends and critics. This rich and original study will be of interest to researchers in the philosophy of Socrates, selfhood, and ancient thought.
Heat/mass transport in shear flow over a heterogeneous surface with first-order surface-reactive domains
Authors: Shah Preyas N. , Shaqfeh Eric S. G.
Published: October 2015

Surfaces that include heterogeneous mass transfer at the microscale are ubiquitous in nature and engineering. Many such media are modelled via an effective surface reaction rate or mass transfer coefficient employing the conventional ansatz of kinetically limited transport at the microscale. However, this assumption is not always valid, particularly when there is strong flow. We are interested in modelling reactive and/or porous surfaces that occur in systems where the effective Damköhler number at the microscale can be $O(1)$ and the local Péclet number may be large. In order to expand the range of the effective mass transfer surface coefficient, we study transport from a uniform bath of species in an unbounded shear flow over a flat surface. This surface has a heterogeneous distribution of first-order surface-reactive circular patches (or pores). To understand the physics at the length scale of the patch size, we first analyse the flux to a single reactive patch. We use both analytic and boundary element simulations for this purpose. The shear flow induces a 3-D concentration wake structure downstream of the patch. When two patches are aligned in the shear direction, the wakes interact to reduce the per patch flux compared with the single-patch case. Having determined the length scale of the interaction between two patches, we study the transport to a periodic and disordered distribution of patches again using analytic and boundary integral techniques. We obtain, up to non-dilute patch area fraction, an effective boundary condition for the transport to the patches that depends on the local mass transfer coefficient (or reaction rate) and shear rate. We demonstrate that this boundary condition replaces the details of the heterogeneous surfaces at a wall-normal effective slip distance also determined for non-dilute patch area fractions. The slip distance again depends on the shear rate, and weakly on the reaction rate, and scales with the patch size. These effective boundary conditions can be used directly in large-scale physics simulations as long as the local shear rate, reaction rate and patch area fraction are known.
Energy-consistent entrainment relations for jets and plumes
Authors: van Reeuwijk Maarten , Craske John
Published: October 2015

We discuss energetic restrictions on the entrainment coefficient${\it\alpha}$for axisymmetric jets and plumes. The resulting entrainment relation includes contributions from the mean flow, turbulence and pressure, fundamentally linking${\it\alpha}$to the production of turbulence kinetic energy, the plume Richardson number$\mathit{Ri}$and the profile coefficients associated with the shape of the buoyancy and velocity profiles. This entrainment relation generalises the work by Kaminskiet al. (J. Fluid Mech., vol. 526, 2005, pp. 361–376) and Fox (J. Geophys. Res., vol. 75, 1970, pp. 6818–6835). The energetic viewpoint provides a unified framework with which to analyse the classical entrainment models implied by the plume theories of Mortonet al.(Proc. R. Soc. Lond.A, vol. 234, 1955, pp. 1–23) and Priestley & Ball (Q. J. R. Meteorol. Soc., vol. 81, 1954, pp. 144–157). Data for pure jets and plumes in unstratified environments indicate that to first order the physics is captured by the Priestley and Ball entrainment model, implying that (1) the profile coefficient associated with the production of turbulence kinetic energy has approximately the same value for pure plumes and jets, (2) the value of${\it\alpha}$for a pure plume is roughly a factor of$5/3$larger than for a jet and (3) the enhanced entrainment coefficient in plumes is primarily associated with the behaviour of the mean flow and not with buoyancy-enhanced turbulence. Theoretical suggestions are made on how entrainment can be systematically studied by creating constant-$\mathit{Ri}$flows in a numerical simulation or laboratory experiment.
The evolution of segregation in dense inclined flows of binary mixtures of spheres
Authors: Larcher Michele , Jenkins James T.
Published: October 2015

We consider the evolution of particle segregation in collisional flows of two types of spheres down rigid bumpy inclines in the absence of sidewalls. We restrict our analysis to dense flows and use an extension of kinetic theory to predict the concentration of the mixture and the profiles of mixture velocity and granular temperature. A kinetic theory for a binary mixture of nearly elastic spheres that do not differ by much in their size or mass is employed to predict the evolution of the concentration fractions of the two types of spheres. We treat situations in which the flow of the mixture is steady and uniform, but the segregation evolves, either in space or in time. Comparisons of the predictions with the results of discrete numerical simulation and with physical experiments are, in general, good.
ENT manifestations of alkaptonuria: report on a case series
Authors: Steven R A , Kinshuck A J , McCormick M S , Ranganath L R
Published: October 2015

Background:

Alkaptonuria is an inborn error of metabolism. It is a multisystem disease with characteristic ENT manifestations. This paper reports, for the first time, the ENT findings in a cohort of alkaptonuria patients.

Method:

Patients attending the National Centre for Alkaptonuria (Royal Liverpool and Broadgreen University Hospitals NHS Trust) underwent a full ENT assessment.

Results:

Eighteen of the 20 patients (90 per cent) had an ENT sign or symptom. These included discolouration of the pinna, cerumen, nasal septum and pharynx.

Conclusion:

Discolouration of cerumen may occur before 30 years of age and may therefore be an important early clinical sign. Further audiological assessment of patients is needed to clarify if an association exists between alkaptonuria and hearing loss. Alkaptonuria is a condition that could present to the otolaryngologist. Successful early diagnosis and referral to a specialist centre is essential so that patients can be offered disease-modifying therapy.
Endoscopic-guided injection of botulinum toxin into the cricopharyngeus muscle: our experience
Authors: Sharma S D , Kumar G , Eweiss A , Chatrath P , Kaddour H
Published: October 2015

Objective:

To assess the efficacy of endoscopic-guided botulinum toxin injection into the cricopharyngeus muscle and evaluate the duration of its effects.

Methods:

A 3-year prospective study of 12 patients undergoing injection of botulinum toxin was conducted, with a telephone survey to assess dysphagia pre-operatively, and at 1, 3 and 6 months post-treatment, using the MD Anderson Dysphagia Inventory.

Results:

Median age was 66.2 years. Causes of cricopharyngeal dysphagia included idiopathic cricopharyngeal hypertrophy (67 per cent), previous cerebrovascular accident (17 per cent), cranial nerve palsy (8 per cent) and previous chemoradiotherapy to the neck (8 per cent). There were no complications. Two patients had repeat injections after six months. There was significant improvement in MD Anderson Dysphagia Inventory scores at one and three months versus pre-operative scores (73.1 ± 14.9 vs 46.9 ± 7.6, p = 0.0001, and 65.1 ± 11.5 vs 46.9 ± 7.6, p = 0.0001), but not at six months (51.0 ± 11.0 vs 46.9 ± 7.6, p = 0.14).

Conclusion:

Endoscopic-guided injection of botulinum toxin into the cricopharyngeus muscle is a safe and effective method for treating cricopharyngeal muscle dysfunction, lasting up to six months.
Food consumption patterns of the Vulnerable Socotra cormorant Phalacrocorax nigrogularis indicate minimal overlap with fisheries in the eastern Arabian Gulf
Authors: Muzaffar Sabir Bin , Gubiani Robert , Benjamin Sonya , AlShihi Rashid , Al-Romithi Ahmad , Al Kaabi Faisal Humaid
Published: October 2015

Historically cormorants have come into direct conflict with fisheries because of their feeding habits. The Socotra cormorant Phalacrocorax nigrogularis is a regionally endemic seabird restricted to the Arabian Gulf and coastal Oman, and is categorized as Vulnerable on the IUCN Red List. The species is perceived as being detrimental to local fisheries and is therefore persecuted. We studied the diet and estimated the number of Socotra cormorants breeding on Siniya Island, Umm Al Quwain, United Arab Emirates, to determine their impact on local fisheries. Regurgitated fish loads were collected during the breeding seasons of 2011–2012 and 2012–2013, and the biomass of fish consumed by the Socotra cormorant population was estimated. The diet comprised seven species of fish, with sailfin flying fish Parexocoetus mento and blue-stripe sardine Herklotsichthys quadrimaculatus dominating in 2011–2012 and anchovies (Encrasicholina spp.) dominating in 2012–2013. Biomass of fish loads was higher in 2011–2012 compared to 2012–2013, although mean biomass of fish loads did not differ. Dominant fish species in the cormorant's diet were either used as baitfish or were non-target species in the fisheries. The low overlap between the diet of the Socotra cormorant and the fish landed by the fisheries suggests that the cormorant's impact on fisheries is minimal. Conservation strategies to protect the birds and their feeding areas could safeguard populations of this species from further declines.
Kant on Religious Moral Education
Authors: vanden Auweele Dennis
Published: October 2015

While scholars are slowly coming to realize that Kant’s moral philosophy has a distinctive theory of moral education, the import of religion in such education is generally neglected or even denied. This essay argues that Kant’s reflections on religion in parts II and III ofReligion within the Boundaries of Mere Reasoninterpret religion specifically as one aspect of moral education, namely moral ascetics. After first clearly distinguishing between a cognitive and a conative aspect of moral education, I show how certain historical religious practices serve to provide the conative aspect of moral education. Kant defines this aspect of moral education as practices that render the human agent ‘valiantandcheerfulin fulfilling his duties’ (MS, 6: 484). By this it is meant that certain practices can inspire moral interests either by justifying rational hope in living up to a certain standard of moral perfection (Christology) or by endeavouring to unite human beings in a universal, invisible ethical community that inspires cooperation rather than adversity (ecclesiology).
Anoctamin 6 is localized in the primary cilium of renal tubular cells and is involved in apoptosis-dependent cyst lumen formation
Authors: Forschbach V , Goppelt-Struebe M , Kunzelmann K , Schreiber R , Piedagnel R , Kraus A , Eckardt K-U , Buchholz B
Published: October 2015

Primary cilia are antenna-like structures projected from the apical surface of various mammalian cells including renal tubular cells. Functional or structural defects of the cilium lead to systemic disorders comprising polycystic kidneys as a key feature. Here we show that anoctamin 6 (ANO6), a member of the anoctamin chloride channel family, is localized in the primary cilium of renal epithelial cells in vitro and in vivo. ANO6 was not essential for cilia formation and had no effect on in vitro cyst expansion. However, knockdown of ANO6 impaired cyst lumen formation of MDCK cells in three-dimensional culture. In the absence of ANO6, apoptosis was reduced and epithelial cells were incompletely removed from the center of cell aggregates, which form in the early phase of cystogenesis. In line with these data, we show that ANO6 is highly expressed in apoptotic cyst epithelial cells of human polycystic kidneys. These data identify ANO6 as a cilium-associated protein and suggest its functional relevance in cyst formation.
Planar carbon nanotube–graphene hybrid films for high-performance broadband photodetectors
Authors: Liu Yuanda , Wang Fengqiu , Wang Xiaomu , Wang Xizhang , Flahaut Emmanuel , Liu Xiaolong , Li Yao , Wang Xinran , Xu Yongbing , Shi Yi , Zhang Rong
Published: October 2015

Graphene has emerged as a promising material for photonic applications fuelled by its superior electronic and optical properties. However, the photoresponsivity is limited by the low absorption cross-section and ultrafast recombination rates of photoexcited carriers. Here we demonstrate a photoconductive gain of ∼105 electrons per photon in a carbon nanotube–graphene hybrid due to efficient photocarriers generation and transport within the nanostructure. A broadband photodetector (covering 400–1,550 nm) based on such hybrid films is fabricated with a high photoresponsivity of >100 A W−1 and a fast response time of ∼100 μs. The combination of ultra-broad bandwidth, high responsivities and fast operating speeds affords new opportunities for facile and scalable fabrication of all-carbon optoelectronic devices.
Cutoff lensing: predicting catalytic sites in enzymes
Authors: Aubailly Simon , Piazza Francesco
Published: October 2015

Predicting function-related amino acids in proteins with unknown function or unknown allosteric binding sites in drug-targeted proteins is a task of paramount importance in molecular biomedicine. In this paper we introduce a simple, light and computationally inexpensive structure-based method to identify catalytic sites in enzymes. Our method, termed cutoff lensing, is a general procedure consisting in letting the cutoff used to build an elastic network model increase to large values. A validation of our method against a large database of annotated enzymes shows that optimal values of the cutoff exist such that three different structure-based indicators allow one to recover a maximum of the known catalytic sites. Interestingly, we find that the larger the structures the greater the predictive power afforded by our method. Possible ways to combine the three indicators into a single figure of merit and into a specific sequential analysis are suggested and discussed with reference to the classic case of HIV-protease. Our method could be used as a complement to other sequence- and/or structure-based methods to narrow the results of large-scale screenings.
The 82-plex plasma protein signature that predicts increasing inflammation
Authors: Tepel Martin , Beck Hans C. , Tan Qihua , Borst Christoffer , Rasmussen Lars M.
Published: October 2015

The objective of the study was to define the specific plasma protein signature that predicts the increase of the inflammation marker C-reactive protein from index day to next-day using proteome analysis and novel bioinformatics tools. We performed a prospective study of 91 incident kidney transplant recipients and quantified 359 plasma proteins simultaneously using nano-Liquid-Chromatography-Tandem Mass-Spectrometry in individual samples and plasma C-reactive protein on the index day and the next day. Next-day C-reactive protein increased in 59 patients whereas it decreased in 32 patients. The prediction model selected and validated 82 plasma proteins which determined increased next-day C-reactive protein (area under receiver-operator-characteristics curve, 0.772; 95% confidence interval, 0.669 to 0.876; P < 0.0001). Multivariable logistic regression showed that 82-plex protein signature (P < 0.001) was associated with observed increased next-day C-reactive protein. The 82-plex protein signature outperformed routine clinical procedures. The category-free net reclassification index improved with 82-plex plasma protein signature (total net reclassification index, 88.3%). Using the 82-plex plasma protein signature increased net reclassification index with a clinical meaningful 10% increase of risk mainly by the improvement of reclassification of subjects in the event group. An 82-plex plasma protein signature predicts an increase of the inflammatory marker C-reactive protein.
Correction: Corrigendum: The Core Pattern Analysis on Chinese Herbal Medicine for Sjögren’s syndrome: A Nationwide Population-Based Study
Authors: Chang Ching-Mao , Chu Hsueh-Ting , Wei Yau-Huei , Chen Fang-Pey , Wang Shengwen , Wu Po-Chang , Yen Hung-Rong , Chen Tzeng-Ji , Chang Hen-Hong
Published: October 2015

This large-scale survey aimed to evaluate frequencies and patterns of Chinese herbal medicine (CHM) used for Sjögren's syndrome (SS) in Taiwan by analyzing the National Health Insurance Research Database (NHIRD) for cases in which CHM was used as an alternative therapy to Western medicine for improving patients' discomforts. We analyzed cases of SS principal diagnosis (ICD-9:710.2) with a catastrophic illness certificate (CIC) in traditional Chinese medicine (TCM) outpatient clinics from three cohorts of the Longitudinal Health Insurance Database (LHID) in the NHIRD between 2002 and 2011. CHM prescription patterns for SS were evaluated from claimed visitation files and corresponding prescription files. There were 15,914 SS patients with CIC (SS/CIC), and we found only 130 SS/CIC cases visiting TCM clinics in LHID2000, 133 in LHID2005, and 126 in LHID2010. After removing duplicate data, 366 SS/CIC and 4,867 visits were analyzed. The 50–59 year age group showed the highest ratio (29.51%) in both women and men. “Qi-Ju-Di-Huang-Wan” and “Xuan-Shen” (Scrophularia ningpoensis Hemsl.) was the most commonly used formula and single herb, respectively. “Qi-Ju-Di-Huang-Wan, Gan-Lu-Yin, Xuan-Shen, Mai-Men-Dong (Ophiopogon japonicus (L. f.) Ker-Gawl.), and Sheng-Di-Huang (raw Rehmannia glutinosa Libosch)” were the core pattern prescriptions in treating SS/CIC.
Correction: Corrigendum: Development of a transformation system for Hirsutella spp. and visualization of the mode of nematode infection by GFP-labeled H. minnesotensis
Authors: Sun Jingzu , Park Sook-Young , Kang Seogchan , Liu Xingzhong , Qiu Junzhi , Xiang Meichun
Published: October 2015

Hirsutella rhossiliensis and H. minnesotensis are endoparasitic fungi of the second-stage juvenile (J2) of the soybean cyst nematode (Heterodera glycines) in nature. They also parasitize both H. glycines J2 and Caenorhabditis elegans on agar plates. Agrobacterium tumefaciens-mediated transformation conditions were established for these Hirsutella spp. The resulting transformants were similar to the corresponding wild-type strains. The infection processes of H. glycines J2 and C. elegans second larval stage (L2) by H. minnesotensis expressing ZsGreen were microscopically analyzed. Conidia of H. minnesotensis adhered to passing nematodes within 8 h post-inoculation (hpi), formed an infection peg between 8 and 12 hpi, and penetrated the nematode cuticle between 12 and 24 hpi for C. elegans L2 and between 12 and 32 hpi for H. glycines J2. Hyphal proliferation inside of the nematode coelom was observed at approximately 32 hpi for C. elegans L2 and at approximately 40 hpi for H. glycines J2. The fungus consumed the whole body and grew out to produce conidia at approximately 156 and 204 hpi for C. elegans L2 and H. glycines J2, respectively. The efficient transformation protocol and a better understanding of infection process provide a solid foundation for studying the molecular and cellular mechanisms underlying fungal parasitism of nematodes.
Stacked thin layers of metaphase chromatin explain the geometry of chromosome rearrangements and banding
Authors: Daban Joan-Ramon
Published: October 2015

The three-dimensional organization of tightly condensed chromatin within metaphase chromosomes has been one of the most challenging problems in structural biology since the discovery of the nucleosome. This study shows that chromosome images obtained from typical banded karyotypes and from different multicolour cytogenetic analyses can be used to gain information about the internal structure of chromosomes. Chromatin bands and the connection surfaces in sister chromatid exchanges and in cancer translocations are planar and orthogonal to the chromosome axis. Chromosome stretching produces band splitting and even the thinnest bands are orthogonal and well defined, indicating that short stretches of DNA can occupy completely the chromosome cross-section. These observations impose strong physical constraints on models that attempt to explain chromatin folding in chromosomes. The thin-plate model, which consists of many stacked layers of planar chromatin perpendicular to the chromosome axis, is compatible with the observed orientation of bands, with the existence of thin bands and with band splitting; it is also compatible with the orthogonal orientation and planar geometry of the connection surfaces in chromosome rearrangements. The results obtained provide a consistent interpretation of the chromosome structural properties that are used in clinical cytogenetics for the diagnosis of hereditary diseases and cancers.
Correction: Corrigendum: Tailoring the strength and porosity of rapid-hardening magnesia phosphate paste via the pre-foaming method
Authors: Liu Li-Jie , Li Jin-Hong , Wang Xiang , Qian Ting-Ting , Li Xiao-Hui
Published: October 2015

High-porosity magnesia phosphate paste (HPMPP) was prepared via the pre-foaming method. In the pre-foaming method, sintering treatment was not required. The bulk density and maximum compressive strength of the HPMPP prepared according to the ratio of water to solids (W/So) of 0.32 reached 464.00 ± 5.00 Kg/m3 and 0.30 ± 0.05 MPa, respectively. The compressive strength increased with the increases in the addition amounts of sodium silicate and polypropylene fibers. The bulk density of HPMPP increased with the increase in the addition of sodium silicate and decreased with the increase in the addition of polypropylene fibers. Besides, the porosity of the magnesia phosphate paste increased from 79.85% to 81.27% and from 80.31% to 83.75% after the addition of sodium silicate and polypropylene fibers respectively. The highest porosity (83.75%) of the prepared HPMPP was realized under the addition proportion (sodium silicate: polypropylene fibers: solids = 0.06:0.0025:1). The average pore size of the prepared HPMPP is about 180 μm and the pore distribution range is relatively narrow. The hydration product (struvite) is combined with MgO particle one by one and then coated on the surface of bubbles. With the decrease of the water content, after breaking bubbles, the porous structure can be achieved.
Interaction between microRNA expression and classical risk factors in the risk of coronary heart disease
Authors: Ding Xiao-Qing , Ge Peng-Cheng , Liu Zhe , Jia Heng , Chen Xi , An Feng-Hui , Li Li-Hua , Chen Zhao-Hong , Mao Hong-Wei , Li Zhao-Yang , Gu Yan , Zhu Tie-Bing , Li Chun-Jian , Wang Lian-Sheng , Ma Wen-Zhu , Yang Zhi-Jian , Jia En-Zhi
Published: October 2015

The aim of this study was to identify the synergistic effect of microRNA expression with classical risk factors of coronary heart disease (CHD) and to explore their diagnostic value for coronary stenotic lesions in subjects with CHD. Plasma samples were obtained from 66 subjects with CHD and from 58 control individuals. A quantitative reverse-transcription PCR (RT-qPCR) assay was conducted to confirm the relative expressions of the known CHD-related miRNAs. The severity of coronary atherosclerosis was based on the Gensini scoring system. The expression of miR-125b in plasma of the CHD group was lower than that of the non-CHD group (0.14 ± 0.09 vs. 0.18 ± 0.10, p = 0.055) and the miR-125b levels significantly decreased following an increasing Gensini score (P = 0.037). Spearman correlation analyses indicated the Gensini score was negatively associated with miR-125b (r = −0.215, p = 0.017). Of all the miRNAs, miR-125b showed the lowest AUC (0.405; 95% CI: 0.305 ~ 0.506, p = 0.070). We found several synergistic effects between miR-125b and classical risk factors, such as age, sex, CR, FBG and HDL-C; the proportion of CHD attributable to the interaction of miR-125b and age was as high as 80%. Therefore, miR-125b was shown to play an important role in individual’s susceptibility to developing CHD.
A Unified Framework for Reservoir Computing and Extreme Learning Machines based on a Single Time-delayed Neuron
Authors: Ortín S. , Soriano M. C. , Pesquera L. , Brunner D. , San-Martín D. , Fischer I. , Mirasso C. R. , Gutiérrez J. M.
Published: October 2015

In this paper we present a unified framework for extreme learning machines and reservoir computing (echo state networks), which can be physically implemented using a single nonlinear neuron subject to delayed feedback. The reservoir is built within the delay-line, employing a number of “virtual” neurons. These virtual neurons receive random projections from the input layer containing the information to be processed. One key advantage of this approach is that it can be implemented efficiently in hardware. We show that the reservoir computing implementation, in this case optoelectronic, is also capable to realize extreme learning machines, demonstrating the unified framework for both schemes in software as well as in hardware.
Synthesis of an arrayed sgRNA library targeting the human genome
Authors: Schmidt Tobias , Schmid-Burgk Jonathan L. , Hornung Veit
Published: October 2015

Clustered regularly interspaced short palindromic repeats (CRISPR) in conjunction with CRISPR-associated proteins (Cas) can be employed to introduce double stand breaks into mammalian genomes at user-defined loci. The endonuclease activity of the Cas complex can be targeted to a specific genomic region using a single guide RNA (sgRNA). We developed a ligation-independent cloning (LIC) assembly method for efficient and bias-free generation of large sgRNA libraries. Using this system, we performed an iterative shotgun cloning approach to generate an arrayed sgRNA library that targets one critical exon of almost every protein-coding human gene. An orthogonal mixing and deconvolution approach was used to obtain 19,506 unique sequence-validated sgRNAs (91.4% coverage). As tested in HEK 293T cells, constructs of this library have a median genome editing activity of 54.6% and employing sgRNAs of this library to generate knockout cells was successful for 19 out of 19 genes tested.