bluuebunny/crossref_metadata_2025 huggingface dataset:
Additions to the angiospermic flora of Tamil Nadu from Anamalai Hills of Southern Western Ghats
Authors: M. Murugesan, , Hameed S. S. , Premkumar M. , Karthik B. , Manikandan R. , Arisdason W. , Sharief M. U.
Published: November 2023

The present communication deals with the new distributional record of four less known endemic species, namely Cyclea fissicalyx Dunn (Menispermaceae), Impatiens macrocarpa Hook.f. (Balsaminaceae), Impatiens pandata E. Barnes (Balsaminaceae) and Meistera ghatica (K.G. Bhat) Škorničk. & M.F. Newman (Zingiberaceae) to the state of Tamil Nadu. Detailed bibliographic citation, description, flowering and fruiting period and photographic plates have been provided facilitate easy identification of these four species.
Erythrina resupinataRoxb. (Leguminosae:Papilionoideae), a less-known but a potential ornamental from India and Nepal
Authors: Munivenkatappa Sanjappa , Narayanasamy Dhatchanamoorthy , Dinesh Kumar , Dipa Paneru
Published: November 2023

Erythrina resupinata Roxb. has been described with recent collections from India and Nepal. It is a little known species with unique habit within the genus and exhibits sparse distribution. Its IUCN conservation status is provisionally assessed as ‘Endangered’ based on limited field observations and data from herbarium specimens. This species is worth domestication as an ornamental for its attractive bright scarlet flowers arising from perennial rootstock.
The Readiness of Wonosobo and Banjarnegara Towards Open Defecation Free Regencies
Authors: Jati Sutopo Patria , Nurhasmadiar Nandini  , Nikie Astorina Yunita Dewanti  , Nissa Kusariana  , Bimastyaji Surya Ramadan  , Budiyono Budiyono , Pangi Pangi , Aditya Vanessa Rizky
Published: November 2023

Introduction: Open defecation is defined as the practice of defecating in fields, forests, bushes, rivers, beaches, or other open areas, causing soil, water, and air contamination. This can cause environmental pollution and diseases (diarrhea and pneumonia, etc.). Banjarnegara and Wonosobo are the two districts with the lowest percentages of Open Defecation Free (ODF) in Central Java. This study aimed to determine the readiness of Banjarnegara and Wonosobo to become ODF regencies. Methods: This descriptive study used a qualitative approach. Data collection was conducted through Focus Group Discussions (FGD) with policy actors responsible for accelerating ODF in Wonosobo and Banjarnegara. Results and Discussion: This study found that Wonosobo and Banjarnegara were constrained by five issues: policies/regulations and strategies, institutional arrangements, financing, planning and monitoring, and capacity building. A situation analysis involving the Working Group on Housing and Settlement Areas (Pokja PKP) for Wonosobo and Banjarnegara found the ODF's main obstacles, namely coordination and commitment (supply), and community behavior (demand). Conlusion: The readiness of Wonosobo and Banjarnegara as ODF regencies needs to be reviewed, especially the commitment of regional leaders to institution-strengthening policies and public education.
Bir Üniversite Hastanesindeki Sağlık Çalışanlarının Aşılanma Durumları ve Aşılara Karşı Tutumlarının Değerlendirilmesi
Authors: KUCUK Yasin , KARAOĞLU Nazan , KÜÇÜKCERAN Hatice
Published: November 2023

Enfeksiyon hastalıkları açısından çok fazla riske maruz kalan sağlık çalışanlarının etkin bir şekilde aşılanmasının önemi bilinmesine rağmen hala aşılanma oranlarının düşük seyretmesi dikkat çekicidir. Bu çalışmanın amacı da geniş bir bölgede pek çok hastaya hizmet veren bir üniversite hastanesinin çalışanlarının aşılanma durumlarını ve aşıya karşı tutumlarını belirleyerek uygun yol haritasının çizilmesine veri sağlamaktır. Kesitsel, tanımlayıcı araştırmada gönüllü sağlık çalışanları tabakalı örneklem yöntemi ile belirlenerek sosyodemografik özellikleri, aşılara karşı tutumları, aşılanma durumları kendi bildirimleri ile değerlendirildi. Örneklem grubundaki 436 sağlık çalışanının %58,7’si kadın, %53,7’si evli, %24,5’i hekimdi. Aşılanma hakkında 152’si (%34,9) eğitim almıştı. Hepatit B ve hepatit A’ya karşı bağışık olduğunu bilenler sırasıyla 292 (%67) ve 185 (%42,4) idi ve 210’u (%48,1) son on yılda tetanoz aşısı yaptırmıştı. Her yıl düzenli olarak influenza aşısı yaptıranların oranı %7,8 (n=34) iken, COVID-19 aşısı yaptırma oranı %70,0 (n=305) idi. Sağlık çalışanlarının aşılanmaya karşı ortalama tutum puanı 95,80±13,79 ile olumlu yöndeydi. Üniversite seviyesinde alanlarda (96,52±13,21), almayanlara (92,87±15,69) göre (p=0,028), hekimlerde (98,95±9,78) diğer meslek (94,77±14,73) gruplarına göre aşıya yönelik olumlu tutum artmaktadır (p=0,006). Hekimler arasında ise dahili bölümlerde çalışanlarda (100,52±8,05), cerrahi bölümlerde çalışanlara (92,52±13,50) göre tutum puanının yüksek olması dikkat çekiciydi (p=0,003). Bu çalışma influenza, tetanoz ve tam aşılama hedeflenen COVID-19 aşıları açısından sağlık çalışanlarının etkin şekilde aşılanmadığını göstermektedir. Aşılara karşı tutum puanlarının oldukça yüksek olmasına karşı aşılanma oranlarının düşük seyretmesi risklerin bilinmesine karşı korunma davranışının yeterince içselleştirilmediğini göstermektedir. Bu nedenle çalışmanın sonuçları daha etkin ve davranışa yönelik eğitim ve bilgilendirilmelerin kurumlarda sürekli, zorunlu ve takipli olması gerektiğini düşündürmektedir.
Tungsten tailings issues and reprocessing solutions
Authors: Msumange Deus Albert , Msumange Joshua Albert , Bru Kathy , Bourgeois Florent
Published: November 2023

This paper aims to provide an overview of tungsten (W) tailings properties, detrimental impacts of these tailings, approaches to mitigate these impacts, and a presentation of methods to reprocess them to capture their economic value. Since W is widely used in a variety of industries, it has been extensively mined since the 19th century, and the mining continues to generate significant volumes of tailings. Recent data show that global W production stands at 84 kt per year, and more than 100 Mt of W tailings exist containing over 100 kt of WO3. The tailings contain variable amounts of valuable products and deleterious environmental substances. Some of the contained metals are in great demand for the energy transition. However, these tailings usually contain FeS2/Pyrrhotite and FeAsS minerals, which, when exposed to air and water, can produce acid mine drainage. As such, W tailings may pose environmental and human health risks. Globally, the reprocessing of W tailings presents a potential resource that can be regarded as a paradigm of sustainability and circular economy. Flotation, enhanced gravity separation, and wet high-intensity magnetic separation have been reported to be the common approaches to reprocessing W tailings. However, W processing presents particular difficulties owing to complex material properties, such as fine particle size, surface weathering, similarity in surface properties exhibited by gangue materials (fluorite, apatite, calcite), low concentrations of the elements of interest, and poor mineral liberation.
ANTIBACTERIAL ACTIVITY OF KELAKAI AND KATUK LEAVES EXTRACT COMBINATION IN COMPARISON TO 1% Povidone iodine AGAINST Aggregatibacter actinomycetemcomitans
Authors: Rizqita Siti Denty , Krishnawan Firdaus I Wayan Arya , Hatta Isnur
Published: November 2023

Background: Aggregatibacter actinomycetemcomitans is one of the causes of aggressive periodontitis. Povidone iodine mouthwash at 1% concentration can be used to reduce the growth of these bacteria, but long-term use may lead to side effects that initiate the need for an alternative herbal mouthwash. Kelakai leaves contain antibacterial substances such as flavonoid, tannin, alkaloid and steroid, while katuk leaves contain flavonoid, tannin, alkaloid and saponin. Combination of these two extracts can be used to increase the effectiveness produced. Objective: To prove that the antibacterial activity of kelakai and katuk leaves extract has the same effectivity as 1% Povidone iodine against Aggregatibacter actinomycetemcomitans. Methods: True experimental method with post-test only and control group design was applied in this study. The antibacterial activity test was performed using diffusion method in 13 treatment groups with triple repetition. The total samples used were 39 samples. The treatment groups were the combination of kelakai leaves extract with 25%, 50%, 75%, 100% concentration and katuk leaves extract with 20%, 40%, 80% concentration, and 1% Povidone iodine. The parameter measured was the inhibitory zone (mm). Results: Combination of 100% kelakai leaves and 80% katuk leaves extract has the highest inhibitory zone of 17.33 mm. Post Hoc Bonferroni test results showed that the combination of 75% kelakai leaves and 80% katuk leaves extract were the only group that had no significant difference to 1% Povidone iodine. Conclusion: Combination of 75% kelakai leaves extract and 80% katuk leaves extract is statistically equivalent to 1% Povidone iodine against Aggregatibacter actinomycetemcomitans.  Keywords: Aggregatibacter actinomycetemcomitans, inhibitory zone, katuk leaves extract, kelakai leaves extract, 1% Povidone iodine.
ANTIBACTERIAL ACTIVITY OF RAMBAI LEAF EXTRACT (Sonneratia caseolaris) CONCENTRATION 25%, 50%, 75%, AND 100% AGAINST Streptococcus sanguinis
Authors: Putri Nurul Fathanah , Aspriyanto Didit , Erlita Isyana
Published: November 2023

Background: Dental plaque is a soft layer formed by collection of bacteria that is firmly attached to the tooth surface which if left unchecked can cause tooth decay, periodontal disease and dental caries. Streptococcus sanguinis is the first bacteria to form colonization in plaque formation. Plaque can be diminished by using chlorhexidine gluconate 0,2% mouthwash, but in long-term use it can cause side effects, so an alternative herbal-based mouthwash that has minimal side effects is needed. Rambai leaf extract can be used as an alternative mouthwash because it contains antibacterial compounds, namely, flavonoids, tannins, phenols, triterpenoids, and steroids.  Purpose: The purpose of this study was to determine the antibacterial activity of rambai leaf extract at concentrations of 25%, 50%, 75% and 100% against Streptococcus sanguinis bacteria. Methods: This study used true experimental research with post-test only with a control group design using rambai leaf extract 25%, 50%, 75%, 100%, chlorhexidine gluconate 0.2%, and aquadest. The study used 6 groups with 4 repetitions with a total of 24 samples. Each group was incubated for 24 hours at 37°C, then the diameter of the inhibition zone was measured using a calliper. Results: The results of the Mann Whitney test showed that there was a significant difference between each treatment group and there was no significant difference between 100% rambai leaf extract and chlorhexidine gluconate 0.2%. The average diameter of the inhibition zone of rambai leaf extract was 25% by 9.75 mm, 50% by 13.37 mm, 75% by 16.12 mm, 100% by 19.5 mm, chlorhexidine gluconate 0.2% by 19.12 mm and aquadest 0 mm. Conclusion: Rambai leaf extract with concentrations of 25%, 50%, 75%, and 100% had antibacterial activity against Streptococcus sanguinis. Keywords :  Antibacterial Activities, chlorhexidine gluconate 0,2%,  rambai leaves extracts, Streptococcus sanguinis
A RARE CLINICAL SYPHILIS ORAL MANIFESTATION DUE TO SEXUALLY TRANSMITTED DISEASE AMONG HOMOSEXUAL: CASE REPORT
Authors: T. Santo Agustin Ninintowe , Setiadhi Riani , Drakel Fika Faradillah
Published: November 2023

Background: Syphilis is a sexually transmitted disease caused by Treponema pallidum, often found in Human Immunodeficiency Virus (HIV) patients and homosexuals. The oral manifestations are chancres, mucous patches, focal epithelial hyperplasia (FEH) and papilloma/condyloma. FEH is a rare oral mucosa disorder that occurs in immunocompromised patient. Objective: To present a rare oral manifestation in syphilis patient due to sexually transmitted infections. Case: A 25-year-old homosexual male came to Oral Medicine Department complaining of sores on the palate for 3 weeks and 2 weeks later a painless lump appeared. He was an HIV patient and had been taking ARVs since 6 months ago. Pain when opening the mouth, itching on his face and genital area. Intraoral revealed a soft, pedunculated, non-bleeding, mobile mass at the palate with a diameter about 2 cm, had the same color as the surrounding tissue. There was a reddish band-like area on the maxillary anterior gingival margin, suspected as Linear Gingival Erythema (LGE). Case management: 0.2% Chlorhexidine gluconate mouthwash was administered to prevent secondary infection and he was referred to Dermatovenereology and Periodontology Department. HBsAg Immunochromatography (negative), VDRL titer (reactive), TPHA titer (reactive) therefore diagnosis of syphilis was made and he was treated with a single dose Benzathine penicillin G 2.4 million units intramuscular. The oral lesions healed after 1 month therapy. Conclusion: FEH is a rare oral mucosa disorder in syphilis patients. Knowledge of the signs and symptoms of the disease is needed to make a diagnosis and a multidisciplinary approach to achieve successful treatment. Keywords: Oral manifestation, Sexual transmission, Syphilis, Treponemal infection
ANALYSIS OF SEVEN CASES OF ANGULAR CHEILITIS IN CHILDREN WITH BODY MASS INDEX AND BLOOD TEST RESULTS
Authors: Bakar Abu
Published: November 2023

Background: Angular cheilitis is an acute or chronic inflammation at the angular of the lip which is characterized with fissures and the cracks. Objectives: The aim of this paper was to analyze seven cases of angular cheilitis in children with body mass index and blood test results. Case:  There were seven children patients taken by their parents came to Baiturrahmah Academic Dental Hospital with a same complaint on a day. They complained about the injury and pain while open the mouth in the angular of the mouth. Intra oral examination showed the different clinical appearance at the angular of the mouth (white or red lesions and fissured or cracked). Body mass index analysis showed all the patients are underweight (9 -14) and mostly of the patients have a hemoglobin less than normal. Case Management: Pharmacological management was prescribed multivitamin supplements (vitamin B complex in syrup) and prescribed nystatin topically. One week after first visit, the anglar chelitis lesions has healed Conclusion: Low BMI might be the predisposing factor of the diseases. Keywords: Angular Cheilitis, Blood test,  Body mass index
VITAMIN D DEFICIENCY AS A RISK FACTOR OF RECALCITRANT RECURRENT INTRAORAL HERPES (RIH)
Authors: Heldayani Iin , Hidayat Wahyu
Published: November 2023

Background: Recurrent Intraoral Herpes (RIH) is secondary infection due to Herpes Simplex Virus (HSV) which is latent and most often involves lips and oral cavity. RIH infection could be reactivated as triggered by various factors, such as stress, immunosuppressant, hormonal, sunlight exposure or vitamin D deficiency. Objective: To describe vitamin D deficiency as a risk factor of recalcitrant RIH. Case: A 22-year-old woman came to Oral Medicine Clinic of RSUP. Dr. Hasan Sadikin Bandung with complaints of recurrent stomatitis for two years. Her stomatitis was recalcitrant and recurred several times a month. Intraoral examination revealed ulcers with yellowish-white base surrounded by haloerytem on the right of buccal and tongue lateral and oropharynx. Immunoserology laboratory examination showed an increasement of IgG HSV-1 value and vitamin D (25-OH) deficiency. Case Management: Patient was given Acyclovir 200mg 5 times daily for a week and Hyaluronic Acid 0.025% mouthwash as well as education to maintain an oral hygiene. Lesions were healed after 10 days therapy. However, 3 days later, lesion on the right buccal recurred with bigger size. Therapy was continued with additional administration of vitamin D and instructions to increase consumption of fruits, vegetables and to bask in the sun. Lesions of recalcitrant RIH were completely healed on the 5th visit or 45 days’ therapy. Conclusion: Vitamin D plays a role in all of the body's defense mechanisms and could be given to reduce the high recurrence rate of RIH as vitamin D has a protective effect against viral infections, particularly against HSV. Keywords: Deficiency Vitamin D, Herpes Infection, Recalcitrant, Recurrent Intraoral Herpes.
EFEKTIVITAS PENGGUNAAN PESAN DALAM MEDIA KOMUNIKASI PEMASARAN ONLINE
Authors: Purnomo Agustina Multi
Published: November 2023

The use of online marketing media as a marketing communication medium has proliferated. This study examined the effectiveness of used messages in online marketing communication media, websites, and Instagram, to market IndiHome products. This study used elements of the message content (clarity of message content, the relevance of message content to the product being promoted, as well as the availability of visual, textual, and audiovisual information), accuracy between messages and media characters (informative and entertaining information on Instagram, as well as single consistent information in the website media), and the effectiveness of the used of messages (customers' responses to the attractiveness of the message and the encouragement of buying interest after seeing the message). This study found elements of message content and the accuracy of messages with media characters to get a positive response from customers and potential customers. The two factors of using messages in online marketing communication media are considered attractive by customers and potential customers or effective. However, the use of messages did not directly encourage buying interest. This study proposed adding an element of distinction inherent in the product that cannot be overcome by using messages in online marketing communication.
HUBUNGAN SIKAP KERJA DUDUK TERHADAP KELUHAN NYERI PUNGGUNG BAWAH PADA PEKERJA BAGIAN ADMINISTRASI
Authors: Firmansyah Fathoni , Dewi Anggreini Beta Citra , Nabila Sofia , Rachmawati Siti
Published: November 2023

Nyeri punggung bawah atau biasa disebut dengan istilah low back pain (LBP) adalah nyeri yang dirasakan di bagian punggung bawah. Salah satu penyebab adanya keluhan nyeri punggung bawah adalah sikap kerja duduk yang salah pada saat bekerja. Penelitian ini bertujuan untuk mengetahui hubungan sikap kerja duduk terhadap keluhan nyeri punggung bawah pada pekerja bagian administrasi di PT. Madurasa Unggulan Nusantara. Jenis penelitian ini adalah observasional analitik dengan desain cross sectional. Responden diambil dari pekerja bagian administrasi sebanyak 30 responden dengan pengambilan sampel secara total sampling. Rappid Entire Body Assesment digunakan untuk menilai sikap kerja duduk dan kuesioner Oswestry Low Back Pain Disability untuk menilai keluhan nyeri punggung bawah. Analisis data menggunakan uji Pearson Product Moment. Hasil penelitian menunjukkan 30 responden memiliki rata-rata skor sikap kerja duduk adalah 5,94 dan rata-rata nilai keluhan nyeri punggung bawah adalah 9,858 %. Hasil uji korelasi Pearson Product Moment antara sikap kerja duduk terhadap keluhan nyeri punggung bawah didapatkan nilai p-value p = 0,000 (p<0,05) yang berarti terdapat korelasi bermakna antara sikap kerja.
FAKTOR-FAKTOR YANG BERHUBUNGAN DENGAN STRES KERJA PADA PEKERJA FABRIKASI DI PT X TAHUN 2022
Authors: Hakiki Farhan , Ayu Ira Marti , Heryana Ade , Keumala Cut Alia , Utami Desyawati
Published: November 2023

Ada banyak bahaya di tempat kerja yang berdampak ke kesehatan pekerja. Salah satu bahaya yang terabaikan yaitu gangguan psikis yang bisa memicu stress pekerja. Hasil survei pendahuluan menunjukkan sebesar 72,7% pekerja Fabrikasi di PT X mengalami stress berat dan sedang. Pekerjaan fabrikasi memiliki target pekerjaan yang tinggi namun dengan tenggat waktu yang sedikit dan tetap dituntun untuk menjaga kualitas pekerjaan. Penelitian ini bertujuan ini untuk mengetahui faktor-faktor yang berhubungan dengan stres kerja pada pekerja fabrikasi di PT X Tahun 2022. Desain penelitian yaitu cross-sectional dengan besar sampel 50 pekerja bagian fabrikasi PT X. Teknik pengambilan sampel dengan total sampling. Data penelitin akan dianalisis dengan analisis univariat dan bivariat dengan Uji Chi Square dan Fisher’s Exact. Hasil univariat menunjukkan proporsi tertinggi yaitu pekerja mengalami stress tinggi sebanyak 36 pekerja (72,0%), merasakan tuntutan tugas tinggi sebanyak 31 pekerja (62,0%), memiliki umur berisiko sebanyak 39 pekerja (78,0%), memiliki masa kerja baru sebanyak 38  pekerja (76,0%), dan sudah kawin sebanyak 44 pekerja (88,0%). Hasil bivariat menunjukkan terdapat hubungan antara tuntutan tugas (PR=1,84, CI 95% (1,12 – 3,01), dan umur (PR=3,10, CI 95% (1,17 – 8,22) terhadap stress kerja. Selain itu juga ditem,ukan tidak adanya hubungan antara masa kerja (PR=1,38 CI 95% (0,88 – 2,85) dan status perkawinan (PR=1,50 CI 95% (0,66 – 3,40) terhadap stress kerja. Perusahaan perlu memperhatikan tuntutan tugas yang diterima setiap pekerja, diharapkan tuntutan tugas yang diterima oleh pekerja tidak melebihi kapasitasnya.
PENGARUH BEBAN KERJA MENTAL TERHADAP STRESS KERJA PADA PEKERJA WANITA BAGIAN WEAVING
Authors: Dewi Anggreini Beta Citra , Rachmawati Siti , Wardani Aurina Firda Kusuma
Published: November 2023

Translator  Pendahuluan: PT Dan Liris merupakan perusahaan yang bergerak dalam industri textile terpadu. PT Dan Liris memiliki beberapa divisi, salah satunya adalah divisi penenunan (weaving). Operator weaving banyak mendapat tekanan yang tinggi dikarenakan  barang yang dihasilkan harus sesuai dengan  persyaratan klien. Berdasarkan gejala dan tanda fisiologis, perilaku, psikologis dan somatik, stres adalah hasil dari ketidaksesuaian antara seseorang dan lingkungannya, yang mengakibatkan ketidakmampuan mereka untuk menangani secara efektif berbagai tuntutan yang dibebankan pada mereka. Bekerja di bawah tekanan dengan batas waktu tertentu, bekerja dengan beban kerja berlebih, menjadi salah satu faktor yang  memicu terjadinya stres akibat pekerjaan. Hal ini juga yang memicu stress kerja terutama pada pekerja wanita. Metode: Metode yang digunakan dalam penelitian ini adalah observasional analitik, dengan menggunakan pendekatan cross sectional. Pengambilan sample dilakukan dengan cara Purposive sampling. Instrumen yang digunakan dalam penelitian ini adalah lembar kuesioner NASA-Task Load Index (TLX) untuk menilai beban kerja mental, kuesioner Perceived Stress Scale (PSS-10) untuk menilai tingkat stres kerja. Analisis bivariat menggunakan uji korelasi spearman.Hasil: Berdasarkan hasil perhitungan beban kerja mental dan stress kerja yang didapatlkan bahwa tidak terdapat hubungan yang signifikan antara beban kerja mental dan stres kerja dengan nilai p-value= 0,052 dan r = 0,226. Koefisien korelasi bertanda positif yang berarti semakin tinggi beban kerja mental, maka akan diikuti tingginya stres kerja dan sebaliknya semakin rendah beban kerja mental, maka stres kerja juga akan semakin rendah.Kesimpulan: Hasil penelitian ini menunjukkan bahwa beban kerja mental tidak memiliki hubungan yang signifikan dengan stres kerja.Kata Kunci: Beban Kerja Mental; Stress Kerja
FOXO1 is a master regulator of CAR T memory programming
Authors: Mackall Crystal , Doan Alexander , Mueller Katherine , Chen Andy , Rouin Geoffrey , Daniel Bence , Lattin John , Chen Yingshi , Mozarsky Brett , Markovska Martina , Arias-Umana Jose , Hapke Robert Robert , Jung Inyoung , Xu Peng , Klysz Dorota , Bashti Malek , Quinn Patrick , Sandor Katalin , Zhang Wenxi , Hall Junior , Lareau Caleb , Grupp Stephan , Fraietta Joseph , Sotillo Elena , Satpathy Ansuman , Weber Evan
Published: November 2023

Poor CAR T persistence limits CAR T cell therapies for B cell malignancies and solid tumors1,2. The expression of memory-associated genes such as TCF7 (protein name TCF1) is linked to response and long-term persistence in patients3-7, thereby implicating memory programs in therapeutic efficacy. Here, we demonstrate that the pioneer transcription factor, FOXO1, is responsible for promoting memory programs and restraining exhaustion in human CAR T cells. Pharmacologic inhibition or gene editing of endogenous FOXO1 in human CAR T cells diminished the expression of memory-associated genes, promoted an exhaustion-like phenotype, and impaired antitumor activity in vitro and in vivo. FOXO1 overexpression induced a gene expression program consistent with T cell memory and increased chromatin accessibility at FOXO1 binding motifs. FOXO1-overexpressing cells retained function, memory potential, and metabolic fitness during settings of chronic stimulation and exhibited enhanced persistence and antitumor activity in vivo. Paradoxically, TCF1 overexpression failed to enforce canonical memory programs or enhance CAR T cell potency. Importantly, endogenous FOXO1 activity correlated with CAR T and TIL responses in patients, underscoring its clinical relevance in cancer immunotherapy. Our results demonstrate that memory reprogramming through FOXO1 can enhance the persistence and potency of human CAR T cells and highlights the utility of pioneer factors, which bind condensed chromatin and induce local epigenetic remodeling, for optimizing therapeutic T cell states.
Dynamic topology-mediated maturation of β-barrel proteins in BAM-catalyzed folding
Authors: Zhou Ruhong , Xie Teng , Chan Kevin , Pang Jie , Shen Chongrong , Chang Shenghai , Buratto Damiano , Tang Xiaodi , Zhang Xing , Dong Haohao
Published: November 2023

BamA is an essential component of the β-barrel assembly complex (BAM) that catalyzes the insertion and assembly of β-barrel outer membrane proteins (OMPs) in Gram-negative bacteria. BamA adopts two distinct conformations: the inward-open and outward-open β-barrels, regulated by periplasmic domains during different folding stages. However, it remains unclear how these conformational shifts promote the maturation of OMPs. Drawing from our prior work1, we identify a novel folding intermediate, characterized by an inward-open BamA and a non-closing OMP. This intermediate attains stability via enhanced hydrophobic interactions accompanying the topological alterations of the hybrid-barrel. The subsequent folding events of the hybrid-barrel are then sampled through molecular dynamics simulations augmented with enhanced sampling. Remarkably, our observations reveal that the spontaneous closure and separation are regulated by an induced-fit mechanism and supplementary hydrophobic interactions, prominently from the extracellular loop 1 (BL1) of BamA. Further cell viability assays confirm that disruption to BL1’s topology compromises BamA functionality and is detrimental to the bacteria. Taken together, our findings provide insights into the significance of BamA’s dynamic topological shifts during OMP maturation.
Gradient matters via filament diameter-adjustable 3D printing
Authors: Ruan Changshun , Qu Huawei , Gao Chongjian , Liu Kaizheng , Fu Hongya , Liu Zhiyuan , Kouwer Paul , Han Zhenyu
Published: November 2023

Gradient matters with hierarchical structures endow the natural world with excellent integrity and diversity, such as bone, bamboo, and wood. Currently, direct ink writing (DIW) three-dimensional (3D) printing is attracting tremendous interests, and has been used to explore the fabrication of one-dimensional (1D) and two-dimensional (2D) hierarchical structures by adjusting the diameter, spacing, and angle between filaments. However, it is difficult to generate complex 3D gradient matters owing to the inherent limitations of existing methods in terms of available gradient dimension, gradient resolution, and shape fidelity. Here, we report a filament diameter-adjustable 3D printing (FDA-3DP) strategy that enables conventional DIW 3D printers to produce 1D, 2D, and 3D gradient matters with tunable heterogeneous structures by continuously varying the volume of deposited ink on the printing trajectory. In detail, we developed diameter-programmable filaments by customizing the printing velocity and height. To achieve high shape fidelity, we specially added supporting layers with a constant minimum diameter at needed locations. Next, we showcased the multi-disciplinary applications of our approach in creating horizontal, radial and axial gradient structures, letter-embedded structures, metastructures, tissue-mimicking scaffolds, flexible electronics, and time-driven devices. By showing the enormous potential of this strategy, we anticipate that it could be easily extended to a variety of filament-based additive manufacturing technologies and facilitate the development of functionally graded structures.
Responses of streamflow to changes in climate and land use/cover in the typical semi-arid Liu River Basin, China
Authors: Li Mingqian , Wang He , Du Wei , Gu Hongbiao , Zhou Fanchao , Chi Baoming
Published: November 2023

Since the 1950s, China has implemented numerous soil and water conservation measures to control severe soil erosion in the Liu River Basin (LRB). While these measures have protected the upstream soil and water ecological environment, they have led to a sharp reduction in the downstream flow and the deterioration of the river ecological environment. Therefore, it is important to evaluate the impact of soil and water conservation measures on hydrological processes to assess long-term runoff changes. Using hydrological models and sensitivity analyses based on the Budyko hypothesis, this study quantitatively evaluated the effects of climate change, direct water withdrawal, and soil and water conservation measures on runoff in the LRB during different periods. The runoff series was divided into a baseline period (1956–1969) and two altered periods, period 1 (1970–1999) and period 2 (2000–2020). Human activities were the main cause of the decrease in runoff during the altered periods, contributing 86.3% (− 29.61 mm), while the contribution of climate change was only 13.7% (− 4.7 mm). The impact of climate change manifests as a decrease in flood volume caused by a reduction in precipitation during the flood season. Analysis of two flood cases indicated a 66–84% reduction in basin runoff capacity due to soil and water conservation measures in the upstream area. Soil and water conservation measures reduced the peak flow and total flood volume in the upstream runoff area by 77.98 and 55.16%, respectively, even with nearly double the precipitation. The runoff coefficient in the reservoir area without soil and water conservation measures was four times that in the conservation area. These results contribute to the re-evaluation of the soil and water conservation hydrological effects and provide important guidance for water resource planning and water conservation policy formulation in the LRB.
A Monte Carlo study of the Cellular Dosimetry for SNCT Comparison with MIRD S Values
Authors: Zhang Yi , Lan Changlin , Jiang Gong , Wang Jiahao , Yang Xianlin , Wei Yuting , Ge Yujie
Published: November 2023

Background
An interesting isotope, sulfur-33 (a stable isotope), has been proposed as an additional neutron capture or a cooperating target for enhancing boron neutron capture therapy. Recently, 33S was considered as the substitute for boron-10 in neutron capture radiotherapy. The 33S (n, α)30Si reaction emits the α particles useful for neutron capture therapy. Based on the Monte Carlo method, we investigated the dosimetric characteristics of the cellular model of several geometries for the sulfur-33 neutron capture therapy (SNCT).
Methods
Monte Carlo N-Particle (MCNP) was used for simulating the transport of α particles emitted by SNCT from the cellular model surface (CS), cytoplasm (Cy), or nucleus (N) of a single cell model. The model cells were defined as two concentric spheres consisting of the nucleus and cytoplasm. The radius of the cell model was Rc=6 µm Rn=3 µm. The S values (SC←C, SC←CS, SN←N, SN←Cy, SN←CS) were compared to the results with the Medical Internal Radiation Dosimetry (MIRD) method. Finally, S values of the cellular model of several geometries were calculated.
Results
The relative differences of MCNP versus MIRD method S values for SNCT ranged from 0.28% to 4.29% for C to C (SC←C), -2.82% to 0.05% for Cs to C (SC←Cs), 0.54% to 4.42% for N to N (SN←N), 0.72% to 3.14% for Cy to N (SN ←Cy), and -1.76% to 0.36% for CS to N (SN←CS). The ratios of SN←N/ SC←C, SN←CS/SC←C, SN←Cy/ SC←C decreased with increasing the cell and nucleus size.
Conclusion
The S value simulated by MCNP is in agreement with the MIRD method, which is proved to be reliable for the cellular dosimetry of SNCT by the Monte Carlo method. The Model Carlo method can be used instead of the MIRD method to accurately calculate cellular S values, which solves the problem of cell shape limitation in MIRD method (which is suitable for regular spherical cellular model).
Evaluation of Dental Plaque Area with Artificial Intelligence Model
Authors: Yüksel Büşra , Özveren Neslihan , Yeşil Çağrı
Published: November 2023

Objectives
This study aims to assess the diagnostic accuracy of an artificial intelligence (AI) system employing deep learning for identifying dental plaque, utilizing a dataset comprising photographs of permanent teeth.
Materials and Methods
In this study, photographs of 168 teeth belonging to 20 patients aged between 10–15 years, who met our criteria were included. Intraoral photographs were taken of the patients in two stages, before and after the application of the plaque staining agent. To train the AI system to identify plaque on teeth with dental plaque that is not discolored, plaque and teeth were marked on photos with exposed dental plaque. 140 teeth were used to construct the training group, while 28 teeth were used to create the test group. Another dentist reviewed images of teeth with dental plaque that wasn't discolored, and the effectiveness of AI in detecting plaque was evaluated using pertinent performance indicators. To compare the AI model and the dentist's evaluation outcomes, the mean IoU values were evaluated by the Wilcoxon test.
Results
The AI system showed higher performance in our study with a precision of 82% accuracy, 84% sensitivity, 83% F1 score, 87% accuracy, and 89% specificity in plaque detection. The AUC value was found to be 0.922, and the IoU value was 76%. Subsequently, the dentist's plaque diagnosis performance was also evaluated. The IoU value was 0.71, and the AUC was 0.833. The artificial intelligence model showed statistically significantly higher performance than the dentist. (p < 0.05)
Conclusions
The AI algorithm that we developed has achieved promising results and demonstrated clinically acceptable performance in detecting dental plaque compared to a dentist.
Effect of Bleaching Agents on Composite Resins With and Without BIS-GMA.
Authors: Melo María , Dumitrache Bianca , Ghilotti James , Sanz José Luís , Llena Carmen
Published: November 2023

Objective
to evaluate the change in color, hardness and roughness produced by carbamide peroxide (CP) at two different concentrations on two resins.
Materials and Methods
16% or 45% CP was applied to 66 resin discs with and without Bis-GMA. Color was measured with a spectrophotometer, and ΔEab and ΔE00 calculated. Microhardness tester and SEM were used.
Results
In both composites, the a* and b* coordinates tended to red and yellow, respectively, and were significant in the Bis-GMA group (p < 0.05). The ΔEab and ΔE00 were higher in the composite with Bis-GMA, regardless of the treatment received (p < 0.05). The microhardness was reduced in both composites regardless of the PC concentration compared to the control (p < 0.05). 45% CP reduced the microhardness in the resin group with Bis-GMA compared to 16% CP (p < 0.001), but was not significant in the resin without Bis-GMA (p = 1). An increase in roughness was directly proportional to the concentration of CP and it was more notable in the composite without Bis-GMA.
Conclusions
The composite with Bis-GMA showed a greater tendency to darken than the one without Bis-GMA. The surface hardness of the composite was reduced in both composites and was not influenced by CP concentration in the composite without Bis-GMA.
Clinical relevance:
Bleaching is a common procedure nowadays. It is important to know how how CP affects composites to establish a prognosis of the treatments, both in terms of color change, roughness or hardness.
Passing Path Predicts Shooting Outcome in Football
Authors: Cao Shun
Published: November 2023

What determines the outcome of a shot (scored or unscored) in football (soccer)? Numerous studies have investigated various aspects of this question, including the skills and physical/mental state of the shooter or goalkeeper, the positional information of shots, as well as the attacking styles and defensive formations of the opposing team. However, a critical question has received limited attention: How does the passing path affect the outcome of a shot? In other words, do different paths of the ball before shooting significantly influence the result of shots occurred in the same location? This study aims to fill the gap in the literature by conducting qualitative studies using a dataset comprising 34,938 shots, along with passing paths from top-tier football leagues and international competitions such as the World Cup. Eighteen path features were extracted and applied to three different machine-learning models. The results indicate that the passing path, whether with or without the positional information of shots, can indeed predict shooting outcomes and reveal influential path features. Moreover, it suggests that taking quick actions to move the ball across areas with a high probability of scoring a goal can significantly increases the chance of a successful shot. Interestingly, certain path features that are commonly considered important for team performance, such as the distribution of passe among players and the overall path length, were found to be less significant for shooting outcomes. These findings enhance our understanding of the effective ball-passing and provide valuable insights into the critical factors for achieving successful shots in football games.
Sustainable Synthesis of Dibutyl Itaconate from Biomass Derived Acid via Esterification Reaction over Hierarchical Zeolite H-BEA Catalysts
Authors: Lodhi Aayushi , Dalai Ajay , Maheria Kalpana
Published: November 2023

The current study concentrates on the synthesis of dibutyl itaconate (DBI) via esterification reaction of itaconic acid (ITA) and n-butanol over the synthesized novel hierarchical zeolite and the parent H-BEA acid catalysts. ITA is among the top platform scaffolds which are derived from biomass. DBI, has numerous industrial applications as, plasticizers, gelation accelerators, lubricants, antirust additives, adhesives, detergent additives etc. In the present study, tetradecyltrimethylammonium bromide (TTAB) surfactant is used as a structure directing agent and yeast as an additional modifier to create hierarchical zeolite H-BEA. Several characterization techniques [XRD, SEM-EDX, N2-sorption isotherms, NH3-TPD, FT-IR, solid-state NMR (27Al, 29Si, 1H)] were used to characterise the synthesized hierarchical structure involving both, microporosity and mesoporosity. Under optimal reaction conditions, hierarchical zeolite shows a higher % ITA yield as compared to its counterpart, parent H-BEA zeolite catalyst. This may be attributed to the enhanced physicochemical and catalytic properties of the resulting hierarchical zeolite catalyst.
Reverse effect of Wavelets on ES and ARIMA Models using electricity demand time series data
Authors: Kumar Mohit , Kumar Jatinder
Published: November 2023

For effective energy management and resource planning, accurate electricity demand forecasts are essential. Time series forecasting methods like Exponential Smoothing (ES) and ARIMA models have grown popular recently because of their capacity to identify complex seasonal trends in data [1]. This study aims to compare the performance of different wavelet families in conjunction with ES and ARIMA models for electricity demand forecasting and we are able to see the reverse effect of wavelet. The evaluation of the forecasting models is performed using multiple performance metrics called as KPIs measures such as Mean Absolute Error (MAE), Root Mean Squared Error (RMSE), Mean Absolute Percentage Error (MAPE) and Mean Absolute Scale Error (MASE). The results are compared to assess the accuracy and effectiveness of different wavelets in improving the forecasting performance of ES and ARIMA models. The findings of this study contribute to a better understanding of the applicability of wavelet-based approaches for electricity demand forecasting.
Widespread link between DNA-packing density distribution and TAD boundary locations
Authors: MENG Luming , SHEONG Fu Kit , LUO Qiong
Published: November 2023

DNA is heterogeneously packaged into chromatin, which are further organized into Topologically associating domains (TADs) with sharp boundaries. The locations of TAD boundaries are critical for gene regulation. Here, we investigate whether the distribution of DNA-packing density along chromatin has an impact on the TAD boundary locations. We develop a polymer-physics-based model that utilizes DNA-accessibility data to parameterize DNA-packing density along chromosomes, treating them as heteropolymers, and simulates the stochastic folding of these heteropolymers within the nucleus to yield a conformation ensemble. Such an ensemble accurately reproduces a subset (over 36%) of TAD boundaries in human cells at a genome-wide scale, as confirmed by Hi-C data. Additionally, it faithfully reproduces the spatial distance matrices of 2-Mb genomic regions as provided by FISH experiments. Furthermore, our model demonstrates that utilizing solely DNA-accessibility data as input is already adequate to predict the emergence and disappearance of crucial TADs during early T cell differentiation. These results establish a link between DNA-packing density distribution and TAD boundary positions, complementing existing models and uncovering aspects of genome organization beyond molecular processes such as loop extrusion and phase separation. In the future, integrating these models offers promising avenues for understanding intricate genome organization.
Heat stress releases arachidonic acid to induce autophagy in Sertoli cells by enhancing ROS-mitochondrial-endoplasmic reticulum stress axis
Authors: Hu Yu , Luo NanJian , Gan Lu , Xue HongYan , Zhang JiaoJiao , Wang XianZhong
Published: November 2023

As a key factor in determining testis size and sperm number, Sertoli cells (SCs) play a crucial role in male infertility. Under heat stress (HS), the reduction of SCs counts will negatively impact energy transport and supply to germ cells, leading to the spermatogenesis failure in humans and animals. However, how HS affects the number of SCs remains unclear. To address this, we hypothesized that the changes in SC metabolism could contribute to the adverse effects of HS. In this study, we first found that arachidonic acid (AA), an unsaturated fatty acid, was upregulated post HS exposure through LC-MS/MS metabolome detection. By measuring ROS and MDA levels, as well as expression levels of LC3, LAMP2 and P62, we showed that 100 µM of AA negatively affected the morphology of the testis and accelerated mitochondrial and endoplasmic reticulum damage in association with activating oxidative stress (OS). In line with this, we also observed an adverse effect of AA under HS on the accumulation of Ca2+, the increased expression of mitochondrial electron transport chain (ETC) proteins Complex I, II, V, as well as endoplasmic reticulum stress (ERS) unfolding proteins P-IRE1 and P-PERK. However, pretreatment with 5mM NAC (ROS inhibitor), 150 nM Rotenone (mitochondrial stress inhibitor), and 2.5 mM 4-PBA (ERS inhibitor) could reversed the autophagy induced by AA. Together, it is likely that AA modulates autophagy in SCs through ROS-mitochondrial-endoplasmic reticulum stress axis during HS, which may help to unravel the underlying mechanisms behind male infertility caused by high temperatures.
Selective Adsorption of Methyl Orange Dye by Novel Synthetic Crosslinked-Polydopamine/Polyaniline Nanoparticles
Authors: Gholizadeh Mohammad Reza , Haddadi-Asl Vahid , Ahmadi Hanie , Moussaei Majid
Published: November 2023

A novel technique known as water-in-water emulsion was utilized to synthesize the cross-linked nanoparticles of polydopamine@polyaniline (CPDA@PANI). The synthesized nanoparticles demonstrated their ability to selectively adsorb methyl orange (MO) from aqueous media. Solution pH and contact time are the influential factors employed to ascertain the optimal conditions for organic dye adsorption. Both pseudo-first and pseudo-second-order kinetic models were utilized to fit the adsorption kinetics. Four isothermal adsorption models were used to find the adsorption kinetics model, including Dubnin-Radushkevich, Temkin, Freundlich, and Harkins-Jura. The results revealed that the nanoparticles presented a higher adsorption capacity of 114.48 mgr/gr in acidic medium than in alkaline conditions with dye removal efficiency of 95.4%. This phenomenon can be explained by protonating PANI, which leads to a strong electrostatic attraction between amino and imine groups with MO. By conforming to the pseudo-second-order kinetics model, the adsorption kinetics process displayed a favorable fit, and it was found that the Freundlich isotherm model was more effective for monitoring and assessing adsorption behavior than other available models. It can be derived from this analysis that the absorption of the dye in CPDA@PANI nanoparticles can be categorized as a single-layer adsorption process with homogeneous absorption sites on the nanoparticles.
A novel 1D model approach to optimize the entrainment ratio of a steam ejector and introducing novel definitions of ejector efficiency
Authors: akbarnejad saeed , ZIABASHARHAGH Masoud
Published: November 2023

This paper introduces a novel 1D model approach to optimize a steam ejector entrainment ratio. New definitions of ejector efficiency are defined and the increase in ejector efficiency is studied using this novel 1D model approach. Based on this novel 1D model, an ejector is designed for specified boundary conditions for which CFD entrainment ratio results are available. The ejector's dimensions and geometry are calculated. Based on the main dimensions extracted from the theoretical 1D model, dozens of geometries and dimensions were used to calculate the entrainment ratio using computational fluid dynamics (CFD). As compared to previous literature, the optimal design had a significantly higher entrainment ratio. The entrainment ratio closely approximated the theoretical maximum entrainment ratio as determined by the innovative 1D model approach, given the identical boundary conditions.This paper introduces innovative definitions of ejector efficiency. These definitions can be used to evaluate how well an ejector is designed and how far it can be improved.