bluuebunny/crossref_metadata_2025 huggingface dataset:
Expansion of the mutation spectrum and phenotype of USP7-related neurodevelopmental disorder
Authors: Zheng Hong , Mei Shiyue , Li Fuwei , Wei Liwan , Wang Yanchu , Huang Jinrong , Zhang Feng , Huang Jia , Liu Yanping , Gu Weiyue , Liu Hongyan
Published: November 2022

Background

Hao-fountain syndrome (HAFOUS) is a neurodevelopmental syndrome characterized by global developmental and severe language delays, behavioral abnormalities (including autism), and mild dysmorphic impairment of intellectual development. It is a dominant genetic disease caused by USP7 gene (*602519) mutations on chromosome 16p13.2. So far, only 15 cases with 14 deleterious variants in the USP7 gene have been reported.

Materials and methods

This study describes three unrelated patients with USP7 variants. Besides, we identified novel de novo heterozygous USP7 variants using trio-whole exome sequencing and verified by Sanger sequencing. Furthermore, clinical characteristics were evaluated by reviewing the medical records.

Results

The three identified variants, i.e., one frameshift variant (c.247_250del, p.Glu83Argfs × 18) and two missense variants (c.992A > G, p.Tyr331Cys; c.835T > G, p.Leu279Val) are unreported. The predominant clinical manifestations of the three patients included: DD/ID; language impairment; abnormal behavior; abnormal brain magnetic resonance (dilation of lateral ventricles, dilation of Virchow-Robin spaces, dilated the third ventricle, abnormal cerebral white matter morphology in bilateral occipital lobes, hypodysplasia of the corpus callosum, arachnoid cyst, delayed myelination, and widened subarachnoid space); some also had facial abnormalities.

Conclusion

In summary, DD/ID is the most prevalent clinical phenotype of HAFOUS, although some patients also exhibit language and behavioral abnormalities. For the first time in China, we identified three variants of the USP7 gene using whole-genome sequence data. This work expands the USP7 gene mutation spectrum and provides additional clinical data on the clinical phenotype of HAFOUS.
Metabolism-associated molecular classification of gastric adenocarcinoma
Authors: Ye Yuqing , Yang Wenyun , Ruan Xinjia , Xu Li , Cheng Wenxuan , Zhao Mengmeng , Wang Xin , Chen Xinyi , Cai Daren , Li Guanjie , Wang Yuhang , Yan Fangrong , Lu Xiaofan , Jiang Liyun
Published: November 2022

Most gastric cancers (GC) are adenocarcinomas, whereas GC is a highly heterogeneous disease due to its molecular heterogeneity. However, traditional morphology-based classification systems, including the WHO classification and Lauren’s classification, have limited utility in guiding clinical treatment. We performed nonnegative matrix factorization (NMF) clustering based on 2752 metabolism-associated genes. We characterized each of the subclasses from multiple angles, including subclass-associated metabolism signatures, immune cell infiltration, clinic10al characteristics, drug sensitivity, and pathway enrichment. As a result, four subtypes were identified: immune suppressed, metabolic, mesenchymal/immune exhausted and hypermutated. The subtypes exhibited significant prognostic differences, which suggests that the metabolism-related classification has clinical significance. Metabolic and hypermutated subtypes have better overall survival, and the hypermutated subtype is likely to be sensitive to anti-PD-1 immunotherapy. In addition, our work showed a strong connection with previously established classifications, especially Lei’s subtype, to which we provided an interpretation based on the immune cell infiltration perspective, deepening the understanding of GC heterogeneity. Finally, a 120-gene classifier was generated to determine the GC classification, and a 10-gene prognostic model was developed for survival time prediction.
Comprehensive dosimetric and clinical evaluation of lexicographic optimization-based planning for cervical cancer
Authors: Trivellato Sara , Caricato Paolo , Pellegrini Roberto , Montanari Gianluca , Daniotti Martina Camilla , Bordigoni Bianca , Faccenda Valeria , Panizza Denis , Meregalli Sofia , Bonetto Elisa , Arcangeli Stefano , De Ponti Elena
Published: November 2022

Aim

In this study, a not yet commercially available fully-automated lexicographic optimization (LO) planning algorithm, called mCycle (Elekta AB, Stockholm, Sweden), was validated for cervical cancer.

Material and methods

Twenty-four mono-institutional consecutive treatment plans (50 Gy/25 fx) delivered between November 2019 and April 2022 were retrospectively selected. The automatic re-planning was performed by mCycle, implemented in the Monaco TPS research version (v5.59.13), in which the LO and Multicriterial Optimization (MCO) are coupled with Monte Carlo calculation. mCycle optimization follows an a priori assigned priority list, the so-called Wish List (WL), representing a dialogue between the radiation oncologist and the planner, setting hard constraints and following objectives. The WL was tuned on a patient subset according to the institution’s clinical protocol to obtain an optimal plan in a single optimization. This robust WL was then used to automatically re-plan the remaining patients. Manual plans (MP) and mCycle plans (mCP) were compared in terms of dose distributions, complexity (modulation complexity score, MCS), and delivery accuracy (perpendicular diode matrices, gamma analysis-passing ratio, PR). Their clinical acceptability was assessed through the blind choice of two radiation oncologists. Finally, a global quality score index (SI) was defined to gather into a single number the plan evaluation process.

Results

The WL tuning requested four patients. The 20 automated re-planning tasks took three working days. The median optimization and calculation time can be estimated at 4 h and just over 1 h per MP and mCP, respectively. The dose comparison showed a comparable organ-at-risk spare. The planning target volume coverage increased (V95%: MP 98.0% [95.6–99.3]; mCP 99.2%[89.7–99.9], p >0.05). A significant increase has been registered in MCS (MP 0.29 [0.24–0.34]; mCP 0.26 [0.23–0.30], p <0.05) without affecting delivery accuracy (PR (3%/3mm): MP 97.0% [92.7–99.2]; mCP 97.1% [95.0–98.6], p >0.05). In the blind choice, all mCP results were clinically acceptable and chosen over MP in more than 75% of cases. The median SI score was 0.69 [0.41–0.84] and 0.73 [0.51–0.82] for MP and mCP, respectively (p >0.05).

Conclusions

mCycle plans were comparable to clinical manual plans, more complex but accurately deliverable and registering a similar SI. Automated plans outperformed manual plans in blinded clinical choice.
Case report: Kidney perivascular epithelioid cell tumor treated with anti-VEGFR tyrosine kinase inhibitor and MTOR inhibitor
Authors: Zhang Ninggang , Ren Yaqiong , Zan Likun , Zhang Xuting , Zhao Jian , Wen Lu , Wang Yusheng
Published: November 2022

Perivascular epithelioid cell tumors (PEComas) are rare mesenchymal tumors arising from perivascular epithelial cells. There was no standard treatment for unresectable PEComa before 2021. For a low incidence and a rarely curable disease, development of new therapy is essential. A 45-year-old female was diagnosed with malignant renal PEComa (likely with TFE3 rearrangement) that underwent rapid progression after 10 months of surgery. The patient then received the tyrosine kinase inhibitor (TKI) Apatinib, and the tumor remained stable for 15 months before another progression. The patient then received the MTOR inhibitor everolimus that alleviated her symptoms but the tumor went into remission again after another 15 months. This result suggests that antagonizing the vascular endothelial growth factor receptor (VEGFR) pathway be a useful strategy for malignant PEComas, along with the MTOR pathway inhibition that had recently been approved for the rare tumor.
Glucose-lowering effect of berberine on type 2 diabetes: A systematic review and meta-analysis
Authors: Xie Wenting , Su Fugui , Wang Guizhong , Peng Zichong , Xu Yaomin , Zhang Yi , Xu Ningning , Hou Kaijian , Hu Zhuping , Chen Yan , Chen Rongping
Published: November 2022

Background: Insulin secretory agents are commonly used to treat type 2 diabetes. However, traditional insulin secretory agents such as sulfonylureas and glinides have side effects of hypoglycemia. In recent years, researchers have discovered that berberine can inhibit the voltage-gated k+ channels of pancreatic β cell membrane and promote insulin secretion without causing hypoglycemia, because the glucose-lowering effects of berberine are only under hyperglycemic conditions or in a high-glucose-dependent manner. In order to shed light on the glucose-lowing effects of berberine in type 2 diabetes with different baseline fasting plasma glucose (FPG) and glycosylated hemoglobin (HbA1c), we conducted a meta-analysis of randomized controlled trials.

Methods: We searched eight databases, which included PubMed, EMBASE, Web of Science, the Cochrane Library, and the Chinese databases such as Sino-Med, China National Knowledge Infrastructure (CNKI), Wanfang Database, and VIP Database for Chinese Technical Periodicals, for randomized controlled trials, with berberine as the intervention and patients with type 2 diabetes mellitus as subjects, published up until November 2021. We analyzed the glucose-lowing effects of berberine, including its effects on FPG, HbA1c and 2-h plasma blood glucose (2hPBG), by calculating weighted mean differences (WMD) and 95% confidence interval (CI). To assess the safety of berberine, we analyzed the incidence of total adverse events and hypoglycemia by calculating relative risk (RR) and 95% CI.

Results: Thirty-seven studies involving 3,048 patients were included in the meta-analysis. The results showed that berberine could reduce FPG (WMD = -0.82 mmol/L, 95% CI (-0.95, -0.70)), HbA1c (WMD = -0.63%, 95% CI (-0.72, -0.53)), and 2hPBG (WMD = -1.16 mmol/L, 95% CI (-1.36, -0.96)), with all results being statistically significant. Subgroup analyses revealed that the glucose-lowering effect of berberine was associated with baseline mean FPG and HbA1c in type 2 diabetes. In addition, berberine alone or in combination with oral hypoglycemic agents (OHAs) in the treatment of T2DM did not significantly increase the incidence of total adverse events (RR = 0.73, 95% CI (0.55, 0.97), p = 0.03) and the risk of hypoglycemia (RR = 0.48, 95% CI (0.21, 1.08), p = 0.08).

Conclusion: Berberine has a glucose-lowering effect, which is related to the baseline FPG and HbA1c levels of patients. Treatment with berberine may be safe since it does not increase the incidence of total adverse events and the risk of hypoglycemia.

Systematic Review Registration:https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=292975, identifier CRD42021292975.
Yangyinqingfei decoction attenuates PM2.5-induced lung injury by enhancing arachidonic acid metabolism
Authors: Tang Chunlan , Tang Yuqing , Wang Qinwen , Chu Donghui , Zhou Jinyue , Zhou Yuping
Published: November 2022

Yangyinqingfei Decoction (YYQFD), a traditional Chinese prescription, is well known in the treatment of diphtheria and lung-related diseases in clinic. However, whether it can be used to block the lung injury caused by air pollutant remains unclear. In the present study, the effect of YYQFD was addressed using a PM2.5-induced lung injury mice model. It was shown that YYQFD significantly improved pulmonary functions of mice exposed to PM2.5, the levels of IL-6, TNF-α and MDA were decreased while SOD levels were increased in serum and bronchoalveolar fluid. The potential mechanism of YYQFD was then delved using metabolomic and proteomic techniques. The protein-metabolite joint analysis showed that YYQFD regulated the biosynthesis of unsaturated fatty acids, linoleic acid and arachidonic acid metabolism, causing a significant decrement of pro-inflammatory mediator arachidonic acid with its downstream metabolites like 20-HETE, prostaglandin E2, accompanied by the up-regulation of PTGES2, GPX2 and CBR3 in lung tissue. These data were used to construct a regulatory metabolic network map in terms of the therapeutic role of YYQFD in PM2.5-induced lung injury, thereby provided a novel insight into potential application in the respiratory diseases caused by air pollutants.
Photosynthetic and yield responses of rotating planting strips and reducing nitrogen fertilizer application in maize–peanut intercropping in dry farming areas
Authors: Han Fei , Guo Shuqing , Wei Song , Guo Ru , Cai Tie , Zhang Peng , Jia Zhikuan , Hussain Sadam , Javed Talha , Chen XiaoLi , Ren Xiaolong , Al-Sadoon Mohammad Khalid , Stępień Piotr
Published: November 2022

Improving cropping systems together with suitable agronomic management practices can maintain dry farming productivity and reduce water competition with low N inputs. The objective of the study was to determine the photosynthetic and yield responses of maize and peanut under six treatments: sole maize, sole peanut, maize–peanut intercropping, maize–peanut rotation–intercropping, 20% and 40% N reductions for maize in the maize–peanut rotation–intercropping. Maize–peanut intercropping had no land-use advantage. Intercropped peanut is limited in carboxylation rates and electron transport rate (ETR), leading to a decrease in hundred-grain weight (HGW) and an increase in blighted pods number per plant (NBP). Intercropped peanut adapts to light stress by decreasing light saturation point (Isat) and light compensation point (Icomp) and increasing the electron transport efficiency. Intercropped maize showed an increase in maximum photosynthetic rate (Pnmax) and Icomp due to a combination of improved intercellular CO2 concentration, carboxylation rates, PSII photochemical quantum efficiency, and ETR. Compare to maize–peanut intercropping, maize–peanut rotation–intercropping alleviated the continuous crop barriers of intercropped border row peanut by improving carboxylation rates, electron transport efficiency and decreasing Isat, thereby increasing its HGW and NBP. More importantly, the land equivalent ratio of maize–peanut rotation–intercropping in the second and third planting years were 1.05 and 1.07, respectively, showing obvious land use advantages. A 20% N reduction for maize in maize–peanut rotation–intercropping does not affect photosynthetic character and yield for intercropped crops. However, a 40% N reduction decreased significantly the carboxylation rates, ETR, Icomp and Pnmax of intercropped maize, thereby reducing in a 14.83% HGW and 5.75% lower grain number per spike, and making land-use efficiency negative.
One size does not fit all. Should gambling loss limits be based on income?
Authors: Langeland Elias , Johnsen Ingvild Faxvaag , Sømme Kaja Kastrup , Morken Arne Magnus , Erevik Eilin Kristine , Kolberg Eirin , Jonsson Jakob , Mentzoni Rune Aune , Pallesen Ståle
Published: November 2022

Background

Previous research has suggested empirically based gambling loss limits, with the goal of preventing gambling related harm in the population. However, there is a lack of studies relating gambling loss limits to individual factors such as income. The current study examines whether gambling loss limits should be income-specific.

Materials and methods

The dataset was derived from three representative cross-sectional surveys of the Norwegian population and consisted of 14,630 gamblers. Four income groups, based on a quartile approximation, were formed. Gambling related harm was measured with the Problem Gambling Severity Index (PGSI), and precision-recall (PR) analyses were used to identify loss limits for the different income groups at two levels of gambling severity: moderate-risk gambling and problem gambling.

Results

For both levels of gambling severity, we found the lowest income group to have the lowest gambling loss limits, and the highest income group to have the highest loss limits, which compared to the loss limits for the total sample, were lower and higher, respectively. Calculating the cut-offs for moderate-risk gamblers, we found a consistently ascending pattern from the lowest to the highest income group. Calculating the cut-offs for problem gamblers, we found a similar pattern except for the two middle income groups.

Conclusion

The results suggest that income moderates empirically derived gambling loss limits. Although replication is required, income-based gambling loss limits may have higher applied value for preventing gambling related harm, compared to general loss limits aimed at the entire population.
Mindfulness-based online intervention on mental health among undergraduate nursing students during coronavirus disease 2019 pandemic in Beijing, China: A randomized controlled trial
Authors: Dai Zhenwei , Jing Shu , Wang Hao , Xiao Weijun , Huang Yiman , Chen Xu , Fu Jiaqi , Pan Chen , Tang Qiuping , Wang Huan , Su Xiaoyou
Published: November 2022

Objective

To evaluate the effect of mindfulness intervention on improving mental health among undergraduate nursing students during the coronavirus disease 2019 (COVID-19) pandemic in China.

Methods

An online mindfulness intervention course named Mindfulness Living With Challenge (MLWC) was developed by the research team, and a randomized controlled trial using MLWC among Chinese undergraduate nursing students was carried out. A total of 120 undergraduate nursing students were randomized into control (60 students) and intervention groups (60 students) via a WeChat mini program. Self-administered questionnaire surveys were conducted at pre- and post-intervention, measuring depression, anxiety, stress, mindfulness, and perceived social support. After intervention, the acceptance of the online mindfulness course among participants was assessed by employing the theory of technology acceptance model (TAM).

Results

Among the enrolled 120 participants, 86.67% (52/60) and 93.33% (56/60) of the intervention and control groups remained completed the trial and the questionnaire surveys. Compared with the control group, the reduction of anxiety and stress symptoms, as well as the improvement of mindfulness level and perceived social support in the MLWC intervention group were statistically significant (P < 0.05), while the change in depression was not statistically significant. The scores of the four dimensions in TAM ranged from (5.88 ± 0.94) to (5.91 ± 0.97).

Conclusion

Online mindfulness intervention implemented in this study is effective in improving mental health among undergraduate nursing students, and they were interested in this intervention.

Clinical trial registration

[www.ClinicalTrials.gov], identifier [ChiCTR2 200058103].
The concept of Narcissistic Personality Disorder–Three levels of analysis for interdisciplinary integration
Authors: Jacobs Kerrin A.
Published: November 2022

In this paper, I distinguish three different levels for describing, and three corresponding ways for understanding, deficient empathy as the core of NPD (Narcissistic Personality Disorder). On the macro level, deficient empathy can be explained as disturbed interpersonal functioning, and is understood as lack ofrecognition. On the meso-level, deficient empathy can be described as psychic disintegration, and can be understood specifically in its dissocial aspects. Psychic disintegration in NPD correlates with somatic changes, i.e., dysfunctional affective empathy and mind-reading on the micro level of description, which is the third level. The “core-deficit-model of NPD” that I outline, while not rejecting reductionist approaches outright, argues in favor of integrating (top-down/bottom-up) functionalist descriptions of empathy into a wider conceptual framework of bio-psycho-social functioning. The “core-deficit-model of NPD” is interdisciplinary, can bypass monodisciplinary skepticism, and removes purported barriers between explaining and understanding the “lack” of empathy as the core of pathological narcissism.
COVID-19 burnout, resilience, and psychological distress among Chinese college students
Authors: Sun YueYi , Zhu ShuYue , ChenHuang GanXin , Zhu LiYa , Yang ShuHan , Zhang XiaoCong , Zheng Zheng
Published: November 2022

Background

Since the outbreak of coronavirus disease 2019 (COVID-19), Chinese college students have spent 3 years dealing with infection prevention. Some students have undergone quarantine due to the detection of new variants of COVID-19 and the rise in cases. This study examines pandemic-related isolation and its psychological impact on Chinese college students and explores the relationships among COVID-19 burnout, resilience, and psychological distress in Chinese college students during the pandemic.

Methods

The COVID-19 Burnout Scale, the Connor-Davidson Resilience Scale, and the Brief Symptom Inventory were used to investigate 388 college students from Nanjing City, China. All participants were enrolled in university after 2019, and they participated in the survey voluntarily via the Internet. Participants were divided into two groups (isolated group vs. non-isolated group) based on whether or not they had been isolated.

Results

(1) Significantly lower scores were found for all factors in the isolated group; (2) COVID-19 burnout significantly negatively predicted resilience and significantly positively predicted psychological distress (anxiety, depression, and somatization symptoms), while resilience significantly negatively predicted psychological distress; and (3) Resilience mediated the relationship between COVID-19 burnout and psychological distress.

Conclusion

Isolation is a risk factor for psychological distress related to COVID-19. Resilience can buffer psychological distress and help improve Chinese college students' wellbeing during the COVID-19 pandemic.
Association of intestinal microbiota markers and dietary pattern in Chinese patients with type 2 diabetes: The Henan rural cohort study
Authors: Wang Guanjun , Lyu Quanjun , Yang Tianyu , Cui Songyang , Niu Kailin , Gu Ruohua , Li Yan , Li Jia , Xing Wenguo , Li Linlin
Published: November 2022

Studies on intestinal microbiota in Chinese type 2 diabetes mellitus (T2DM) patients are scarce and correlation studies with dietary intake are lacking. The case-control study included 150 participants (74 T2DM patients and 76 controls) and microbiome analysis was performed using 16S rDNA sequencing. Principal component analysis was used to determine dietary patterns and correlation analysis was used to evaluate the associations between microbiota diversity, T2DM indicators and dietary variables. Compared to controls, the T2DM group had different gut flora characteristics, including lower alpha diversity, higher Firmicutes/Bacteroidetes ratios, statistically significant beta diversity and other specific bacterial species differences. Gut microbiota was associated with several diabetes-related metabolic markers including HOMA2-β, fasting plasma glucose, HbA1c and fasting insulin. Significant associations were also observed between dietary intake pattern and gut flora. The animal foods pattern scores were positively correlated with the relative abundance of the phylum Fusobacteria, and the vegetarian diet pattern scores were positively correlated with the relative abundance of the phylum Actinobacteria. Phylum Actinobacteria mediated the association of vegetarian diet pattern with fasting insulin and HOMA2-β (all P < 0.05). Composition of intestinal microbiota in Chinese T2DM patients differs from that of control population, and the intestinal flora is affected by dietary intake while being associated with several diabetes-related metabolic markers. The gut microbiota may play an important role in linking dietary intake and the etiology of T2DM.
COVID-19 vaccine response and safety in patients with cancer: An overview of systematic reviews
Authors: Sun Hong , Bu Fengjiao , Li Ling , Zhang Xiuwen , Yan Jingchao , Huang Taomin
Published: November 2022

Background

To date, the COVID-19 pandemic does not appear to be overcome with new variants continuously emerging. The vaccination against COVID-19 has been the trend, but there are multiple systematic reviews on COVID-19 vaccines in patients with cancer, resulting in redundant and sub-optimal systematic reviews. There are still some doubts about efficacy and safety of the COVID-19 vaccine in cancer patients.

Purpose

To identify, summarize and synthesize the available evidence of systematic reviews on response and COVID-19 vaccine safety in patients with cancer.

Methods

Multiple databases were searched from their inception to May 1, 2022 to fetch the relevant articles. Study quality was assessed by AMSTAR2. The protocol of this study was registered on PROSPERO (CRD42022327931).

Results

A total of 18 articles were finally included. The seroconversion rates after first dose were ranged from 37.30–54.20% in all cancers, 49.60–62.00% in solid cancers and 33.30–56.00% in hematological malignancies. The seroconversion rates after second dose were ranged from 65.30–87.70% in all cancers, 91.60–96.00% in solid cancers and 58.00–72.60% in hematological malignancies. Cancer types and types of therapy could influence vaccine response. COVID-19 vaccines were safe and well–tolerated.

Conclusions

This study suggests COVID-19 vaccine response is significantly lower in cancer patients. Number of received doses, cancer types and treatment strategies could influence response of COVID-19 vaccine in cancer patients. COVID-19 vaccines are safe and well–tolerated. Considering the emergence of several new variants of SARS-CoV-2 with potential influence on ongoing vaccination programs, there is a need for booster doses to increase the effectiveness of COVID-19 vaccines.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022327931, identifier CRD42022327931.
Serum polyunsaturated fatty acids and hearing threshold shifts in adults in the United States: A cross-sectional study
Authors: Long Lili , Jia Zhenchao , Tang Xinghua
Published: November 2022

Background

Few studies have evaluated the association between polyunsaturated fatty acids (PUFAs) and hearing levels. This study aimed to investigate the association between serum PUFAs and hearing threshold shifts in US adults.

Methods

We investigated 913 adults from the National Health and Nutrition Examination Survey (NHANES) 2011–2012. Multivariate linear regression analyses were conducted to evaluate associations between PUFA and hearing threshold shifts.

Results

Overall, 11 serum PUFAs were inversely associated with low-frequency thresholds, especially in men, and were positively related to high-frequency thresholds, particularly in the 40–59 years old cohort. Furthermore, some serum PUFAs were positively associated with both hearing threshold subgroups in women.

Conclusion

Some PUFAs tend to be beneficial for low-frequency hearing status and detrimental to the high-frequency hearing threshold. The male sex may play a protective role in this association, while the female sex and middle age may be detrimental in the effect of PUFAs on hearing function.
A cognitive modeling approach to learning and using reference biases in language
Authors: Toth Abigail G. , Hendriks Petra , Taatgen Niels A. , van Rij Jacolien
Published: November 2022

During real-time language processing, people rely on linguistic and non-linguistic biases to anticipate upcoming linguistic input. One of these linguistic biases is known as the implicit causality bias, wherein language users anticipate that certain entities will be rementioned in the discourse based on the entity's particular role in an expressed causal event. For example, when language users encounter a sentence like “Elizabeth congratulated Tina…” during real-time language processing, they seemingly anticipate that the discourse will continue about Tina, the object referent, rather than Elizabeth, the subject referent. However, it is often unclear how these reference biases are acquired and how exactly they get used during real-time language processing. In order to investigate these questions, we developed a reference learning model within the PRIMs cognitive architecture that simulated the process of predicting upcoming discourse referents and their linguistic forms. Crucially, across the linguistic input the model was presented with, there were asymmetries with respect to how the discourse continued. By utilizing the learning mechanisms of the PRIMs architecture, the model was able to optimize its predictions, ultimately leading to biased model behavior. More specifically, following subject-biased implicit causality verbs the model was more likely to predict that the discourse would continue about the subject referent, whereas following object-biased implicit causality verbs the model was more likely to predict that the discourse would continue about the object referent. In a similar fashion, the model was more likely to predict that subject referent continuations would be in the form of a pronoun, whereas object referent continuations would be in the form of a proper name. These learned biases were also shown to generalize to novel contexts in which either the verb or the subject and object referents were new. The results of the present study demonstrate that seemingly complex linguistic behavior can be explained by cognitively plausible domain-general learning mechanisms. This study has implications for psycholinguistic accounts of predictive language processing and language learning, as well as for theories of implicit causality and reference processing.
“Without social there is no health”: Social work perspectives in multidisciplinary healthcare
Authors: Di Rosa Roberta T.
Published: November 2022

The pandemic has not just affected the health sphere: strong social effects of the emergency have added to the health risk, stressing on social relations and the deterioration of people's living conditions, and making those who are already fragile more fragile. Notwithstanding, during the emergency following the COVID-19 pandemic the attention was focused, indeed understandably, on the health aspects, widening the already existing misalignment between the health interventions and the social ones. Emergency oriented efforts and resources more toward a clinical care approach (cure) than toward support for the social and the inclusion aspects (care). Reflecting on the specific area of health care that interacts with social care (and vice versa), shows how the medicalization in managing the emergency have undermined or, at least, weakened the global approach to the person and to vulnerability profiles that should inspire the socio-healthcare integration. The aim of this review is describing the relationship between the health and social systems and the effects of the COVID-19 pandemic on it: a review of studies on the role played by social work in the health sector before and during COVID-19 pandemic emergency shows how much potential there is still to be developed for social work in the health sector that acts together with the personal health services; a care that looks at the person within his or her relationships, community resources and environmental aspects requires an investment toward integration between hospital care, social services and local communities.
Endoscopy-assisted resection of a sphenoid-wing meningioma using a 3D-printed patient-specific pointer in a dog: A case report
Authors: So JaeWon , Lee HaeBeom , Jeong JaeMin , Forterre Franck , Roh YoonHo
Published: November 2022

A 9-year-old female mixed-breed dog presented for treatment of a presumed sphenoid-wing meningioma. Clinical signs included tonic-clonic seizures lasting <1 min, which had started 3 months previously. The physical examination results were unremarkable. An eccentrically located neoplastic cystic structure in the right sphenoid bone region suggestive of a meningioma and peritumoural brain oedema was observed in pre-operative magnetic resonance imaging (MRI). Prior to surgery, a three-dimensional (3D) patient-specific pointer (PSP) was designed using computed tomography (CT) images and computer-aided 3D design software. After a targeted approach and exposure of the lateral part of the right temporal lobe by a craniectomy guided by the 3D-PSP, complete macroscopic piecemeal resection of the meningioma could be performed using endoscopy-assisted brain surgery. Post-operative MRI confirmed complete excision of the tumor. Anticonvulsive therapy was discontinued after 90 days, and the dosage of anticonvulsants was tapered 2 weeks after surgery. At a follow-up examination 225 days post-operatively, recurrence of seizures was not observed, and the absence of tumor recurrence was confirmed by a repeat MRI examination. To the best of our knowledge, this is the first report in veterinary medicine describing a successful resection of a sphenoid-wing meningioma using a 3D-PSP. 3D-PSP-assisted craniectomy may be a surgical option for some canine skull-based tumors, such as sphenoid wing meningiomas.
Estimation of Actual Evapotranspiration and Crop Coefficient of Transplanted Puddled Rice Using a Modified Non-Weighing Paddy Lysimeter
Authors: Kumari Arti , Upadhyaya Ashutosh , Jeet Pawan , Al-Ansari Nadhir , Rajput Jitendra , Sundaram Prem K. , Saurabh Kirti , Prakash Ved , Singh Anil K. , Raman Rohan K. , Gaddikeri Venkatesh , Kuriqi Alban
Published: November 2022

Lysimetric and eddy covariance techniques are commonly used to directly estimate actual crop evapotranspiration (ETa). However, these technologies are costly, laborious, and require skills which make in situ ET estimation difficult, particularly in developing countries. With this in mind, an attempt was made to determine ETa and stagewise crop coefficient (Kc) values of transplanted puddled rice using a modified non-weighing paddy lysimeter. The results were compared to indirect methods, viz., FAO Penman–Monteith and pan evaporation. Daily ETa ranged from 1.9 to 8.2 mmday−1, with a mean of 4.02 ± 1.35 mmday−1, and their comparison showed that the FAO Penman–Monteith equation performed well for the coefficient of determination (R2 of 0.63), root mean squared error (RMSE = 0.80), and mean absolute percentage error (MAPE = 13.6 %), and was highly correlated with ETa throughout the crop season. However, the pan evaporation approach was underestimated (R2 of 0.24; RMSE = 0.98; MAPE = 22.13%) due to a consistent pan coefficient value (0.71), vegetation role and measurement errors. In addition, actual Kc values were obtained as 1.13 ± 0.13, 1.27 ± 0.2, 1.23 ± 0.16, and 0.93 ± 0.18 for the initial, crop development, mid-season, and end-season stages, respectively. These estimated crop coefficient values were higher than FAO Kc values. Statistical analysis results revealed that the overall stagewise-derived average Kc values were in line with FAO values, but different from the derived pan Kc values, although found insignificant at a 5% significance level. In addition, water productivity and agro-meteorological indices were derived to evaluate the cultivar performance in this experiment. Therefore, such a methodology may be used in the absence of weighing lysimeter-derived Kc values. The derived regional Kc values can be applied to improve irrigation scheduling under similar agro-climatic conditions.
Identification of a New Wide-Compatibility Locus in Inter-Subspecific Hybrids of Rice (Oryza sativa L.)
Authors: Zhao Weibo , Zhou Wei , Geng Han , Fu Jinmei , Dan Zhiwu , Zeng Yafei , Xu Wuwu , Hu Zhongli , Huang Wenchao
Published: November 2022

As a special class of rice germplasm, wide-compatibility varieties (WCVs) guarantee the fertility of hybrids when there is cross-fertilization between two subspecies. In this study, Chenghui9348 was identified as a new member of the WCV family that improves pollen fertility in an inter-subspecific hybrid. Cytological analysis showed that the abnormal mitosis of microspores resulted in the sterility of pollens at the early bicellular stage in the inter-subspecific hybrid. Furthermore, the new F12 locus, corresponding to improvements in fertility of the indica-japonica hybrid, was found to co-segregate with the RM1047 marker and associated with a region of approximately 630 kb flanked by the D1101 and D1164 markers on chromosome 12. In this region, two putative genes were predicted as the candidates for wide-compatibility genes (WCGs). Sequence analysis revealed that, compared with indica/japonica alleles, deletion/insertion occurred within exons of both putative genes. Together, the present study identified another new WC locus, F12, and offers more opportunities for further exploitation of inter-subspecific hybrids in rice.
Epigenetic and Genetic Contribution for Expression Bias of Homologous Alleles in Polyploid Sugarcane
Authors: Xue Yajie , Shi Yan , Qi Yuchuan , Yu Hang , Zou Chengwu , Cao Xueying , Sun Cuimin , Chen Baoshan , Wang Haifeng
Published: November 2022

DNA methylation regulates gene expression in eukaryotes, but their roles in gene expression changes in polyploids are poorly understood. Here, we comparatively analyzed the DNA methylation profiles and transcriptome maps of four tissues (leaf, rind, pith, and root) in autopolyploid sugarcane (Saccharum spontaneum). The overall DNA methylation levels were relatively equal and were consistent with the similar genome-wide expression levels of homologous alleles among different homologous chromosomes. However, tetrad alleles showed a large proportion of non-balanced alleles (85–89%) compared with balanced alleles (11–15%). For tetrad alleles, highly-expressed alleles showed lower Ka/Ks values than those in lowly-expressed alleles, indicating that dominant (and non-suppressed) alleles were under strong purifying selection pressure compared to non-dominant (and suppressed) alleles. Specifically, higher DNA methylation levels were found in non-balanced alleles compared to balanced alleles, as well as a higher CG to TG substitution rate, suggesting epigenetic and genetic variation associated with transcription divergence. Moreover, among non-balanced alleles, which were associated with DNA methylation changes, many were involved in several processes relevant to agronomic traits, such as responses to stress and carbohydrate transport. Taken together, our results provide unique epigenetic insights into the transcriptional regulation of homologous alleles, which contribute to the key agronomic traits in sugarcane and facilitate epigenetic studies in other polyploid crops.
Spatial-Temporal Change and Synergy/Trade-Off Relationship of “Production–Living–Ecological” Space along the Sino-Vietnamese Border
Authors: Pang Xiaofei , Zhang Liguo , Li Shicheng , Lu Rucheng , Liu Shaokun
Published: November 2022

The internal trade-off of land space is caused by the competition of land use for multiple functions, such as agricultural production, economic development and ecological protection. In response to this common problem, this study uses a positive and negative assignment method and a more refined evaluation scale to analyze the spatial and temporal variation characteristics and synergy/trade-off relationship of production–living–ecological space in the Sino-Vietnamese border area from 2000 to 2020. The results show that: (1) There was great regional diversity in the evolution characteristics of the Sino-Vietnamese border. Production space (PS) and living space (LS) presented prominent expansion, and ecological space (ES) was shrinking. (2) From 2000 to 2020, the degree of synergy between PS and LS has increased gradually. The trade-off relationship between PS and ES has shown different fluctuation intensities. The synergy/trade-off relationship between LS and ES has changed, and its trade-off intensity has continuously increased. (3) On the spatial scale, the PS and LS trade-off areas are mostly distributed in the central and western regions with higher altitudes, and the spatial pattern of the synergy zone is expanding. The synergy and trade-off relationship between PS and ES are relatively stable over time. The area of the trade-off between LS and ES has expanded gradually. In conclusion, the synergy/trade-off relationship between the PLES functions has strong consistency with regional economic and social development, which can serve as a scientific basis for the healthy and sustainable development of land space.
Naringenin and Its Derivatives—Health-Promoting Phytobiotic against Resistant Bacteria and Fungi in Humans
Authors: Duda-Madej Anna , Stecko Jakub , Sobieraj Jakub , Szymańska Natalia , Kozłowska Joanna
Published: November 2022

Naringenin is a trihydroxyflavanone present in large amount in different citrus fruits, e.g., oranges, pomelos, grapefruits, but also in tomatoes, fenugreek and coffee. It has a wide range of pharmacological and biological effects beneficial to human health. Its antioxidant, anti-cancer, anti-inflammatory, antifungal and antimicrobial activity is frequently reported in scientific literature. In this review we presented the current state of knowledge on the antimicrobial activity of naringenin and its natural and synthetic derivatives as a phytobiotic against resistant Gram-positive and Gram-negative bacteria as well as fungi in humans. Most of the data reported here have been obtained from in vitro or in vivo studies. Over the past few years, due to the overuse of antibiotics, the occurrence of bacteria resistant to all available antibiotics has been growing. Therefore, the main focus here is on antibiotic resistant strains, which are a significant, worldwide problem in the treatment of infectious diseases. The situation is so alarming that the WHO has listed microbial resistance to drugs on the list of the 10 most important health problems facing humanity. In addition, based on scientific reports from recent years, we described the potential molecular mechanism of action of these bioflavonoids against pathogenic strains of microorganisms. As plant-derived substances have been pushed out of use with the beginning of the antibiotic era, we hope that this review will contribute to their return as alternative methods of preventing and treating infections in the epoch of drug resistance.
Andrographolide and 4-Phenylbutyric Acid Administration Increase the Expression of Antimicrobial Peptides Beta-Defensin-1 and Cathelicidin and Reduce Mortality in Murine Sepsis
Authors: Bolatchiev Albert , Baturin Vladimir , Bolatchieva Elizaveta
Published: November 2022

Antibiotic resistance is a global threat and requires the search for new treatment strategies. Natural antimicrobial peptides (AMPs) have pronounced antibacterial, antiviral, antifungal, and antitumor activity. AMPs’ clinical use is complicated by the high synthesis costs and rapid proteolytic degradation. The search for small molecules, inducers of endogenous AMP expression, could become a new approach. Here, we investigated for the first time the effect of seven small molecules (andrographolide, levofloxacin, azithromycin, montelukast, 4-phenylbutyric acid, rosuvastatin and valsartan) on AMP (beta-defensin-1, hBD-1 and cathelicidin, LL-37) serum levels in rats. In control groups, the level of hBD-1 was 295.0 (292.9–315.4) pg/mL, and for LL-37, it was 223.8 (213.3–233.6) pg/mL. Andrographolide (ANDR) and 4-phenylbutyric acid (4-PHBA) administration significantly enhanced the level of both AMPs. The hBD-1 level was 581.5 (476.3–607.7) pg/mL for ANDR and 436.9 (399.0–531.6) pg/mL for 4-PHBA. The LL-37 level was 415.4 (376.2–453.8) pg/mL for ANDR and 398.9 (355.7–410.1) pg/mL for 4-PHBA. Moreover, we have shown that these compounds reduce mortality in a murine model of sepsis caused by a carbapenem-resistant Klebsiella aerogenes isolate. From our point of view, these small molecules are promising candidates for further study as potent AMP inducers. The data obtained allow the development of new strategies to combat antibiotic resistance and infectious diseases.
Bacillus subtilis-Fermented Amomum xanthioides Ameliorates Metabolic-Syndrome-Like Pathological Conditions in Long-Term HFHFD-Fed Mice
Authors: Wang Jing-Hua , Hwang Seung-Ju , Shin Kwang-Soo , Lim Dong-Woo , Son Chang-Gue
Published: November 2022

In modern society, numerous metabolic disorders are widespread globally. The present study aimed to demonstrate whether Bacillus subtilis-fermented Amomum xanthioides (BSAX) exerts anti-metabolic disturbance effects compared with the ethyl acetate fraction of Amomum xanthioides (EFAX), a previously verified functional fraction. Mice fed with a high-fat, high-fructose diet (HFHFD) for 10 wk presented a typical model of metabolic dysfunction, and BSAX significantly attenuated a string of metabolic-syndrome-related pathological parameters, such as body, fat, organ mass, lipid markers (TGs, TC, free fatty acids), and glucose metabolism (glucose, insulin), without influencing appetite. Further, BSAX markedly lowered malondialdehyde (MDA) and ROS in the blood and restored antioxidative parameters (SOD, GSH, and CAT in liver tissue, and total bilirubin in serum) by elevating Nrf2 and HO-1. Moreover, BSAX noticeably restored gut microbiota diversity and normalized lipid-metabolism-associated proteins, including SREBP-1, p-AMPK, and PPAR-α. Generally, most metabolic parameters were improved by BSAX to a greater extent than EFAX, except for liver weight and hepatic TC. In conclusion, BSAX alleviates metabolic dysfunction by enhancing lipid metabolism and antioxidative capacity and is more effective than EFAX. Therefore, the application of high-yield, effective BSAX might be a promising approach for curing and preventing metabolic disorders.
Sex-Dependent Responses to Maternal Exposure to PM2.5 in the Offspring
Authors: Chen Hui , Van Reyk David , Oliveira Annabel , Chan Yik Lung , Town Stephanie EL , Rayner Benjamin , Pollock Carol A , Saad Sonia , George Jacob , Padula Matthew P , Oliver Brian G
Published: November 2022

Objective: Particulate matter (PM) with a diameter of 2.5 μm or less (PM2.5) can cross the blood-placental barrier causing adverse foetal outcomes. However, the impact of maternal exposure to low-levels of PM2.5 on liver health and the metabolic profile is unclear. This study aimed to investigate hepatic responses to long-term gestational low-dose PM2.5 exposure, and whether the removal of PM after conception can prevent such effects. Method: Female Balb/c mice (8 weeks) were exposed to PM2.5 (5 μg/day) for 6 weeks prior to mating, during gestation and lactation to model living in a polluted environment (PM group). In a sub-group, PM2.5 exposure was stopped post-conception to model mothers moving to areas with clean air (pre-gestation, Pre) group. Livers were studied in 13-week old offspring. Results: Female offspring in both PM and Pre groups had increased liver triglyceride and glycogen levels, glucose intolerance, but reduced serum insulin and insulin resistance. Male offspring from only the Pre group had increased liver and serum triglycerides, increased liver glycogen, glucose intolerance and higher fasting glucose level. Markers of oxidative stress and inflammation were increased in females from PM and Pre groups. There was also a significant sex difference in the hepatic response to PM2.5 with differential changes in several metabolic markers identified by proteomic analysis. Conclusions: Maternal PM exposure exerted sex-dependent effects on liver health with more severe impacts on females. The removal of PM2.5 during gestation provided limited protection in the offspring’s metabolism regardless of sex.