bluuebunny/crossref_metadata_2025 huggingface dataset:
Acceptor Stem Differences Contribute to Species-Specific Use of Yeast and Human tRNASer
Authors: Berg Matthew D. , Genereaux Julie , Zhu Yanrui , Mian Safee , Gloor Gregory B. , Brandl Christopher J.
Published: December 2018

The molecular mechanisms of translation are highly conserved in all organisms indicative of a single evolutionary origin. This includes the molecular interactions of tRNAs with their cognate aminoacyl-tRNA synthetase, which must be precise to ensure the specificity of the process. For many tRNAs, the anticodon is a major component of the specificity. This is not the case for the aminoacylation of alanine and serine to their cognate tRNAs. Rather, aminoacylation relies on other features of the tRNA. For tRNASer, a key specificity feature is the variable arm, which is positioned between the anticodon arm and the T-arm. The variable arm is conserved from yeast to human. This work was initiated to determine if the structure/function of tRNASer has been conserved from Saccharomyces cerevisiae to human. We did this by detecting mistranslation in yeast cells with tRNASer derivatives having the UGA anticodon converted to UGG for proline. Despite being nearly identical in everything except the acceptor stem, human tRNASer is less active than yeast tRNASer. A chimeric tRNA with the human acceptor stem and other sequences from the yeast molecule acts similarly to the human tRNASer. The 3:70 base pair in the acceptor stem (C:G in yeast and A:U in humans) is a prime determinant of the specificity. Consistent with the functional difference of yeast and human tRNASer resulting from subtle changes in the specificity of their respective SerRS enzymes, the functionality of the human and chimeric tRNASerUGG molecules was enhanced when human SerRS was introduced into yeast. Residues in motif 2 of the aminoacylation domain of SerRS likely participated in the species-specific differences. Trp290 in yeast SerRS (Arg313 in humans) found in motif 2 is proximal to base 70 in models of the tRNA-synthetase interaction. Altering this motif 2 sequence of hSerRS to the yeast sequence decreases the activity of the human enzyme with human tRNASer, supporting the coadaptation of motif 2 loop–acceptor stem interactions.
Generation of A Mucor circinelloides Reporter Strain—A Promising New Tool to Study Antifungal Drug Efficacy and Mucormycosis
Authors: Binder Ulrike , Navarro-Mendoza Maria Isabel , Naschberger Verena , Bauer Ingo , Nicolas Francisco E. , Pallua Johannes D. , Lass-Flörl Cornelia , Garre Victoriano
Published: December 2018

Invasive fungal infections caused by Mucorales (mucormycosis) have increased worldwide. These life-threatening infections affect mainly, but not exclusively, immunocompromised patients, and are characterized by rapid progression, severe tissue damage and an unacceptably high rate of mortality. Still, little is known about this disease and its successful therapy. New tools to understand mucormycosis and a screening method for novel antimycotics are required. Bioluminescent imaging is a powerful tool for in vitro and in vivo approaches. Hence, the objective of this work was to generate and functionally analyze bioluminescent reporter strains of Mucor circinelloides, one mucormycosis-causing pathogen. Reporter strains were constructed by targeted integration of the firefly luciferase gene under control of the M. circinelloides promoter Pzrt1. The luciferase gene was sufficiently expressed, and light emission was detected under several conditions. Phenotypic characteristics, virulence potential and antifungal susceptibility were indifferent to the wild-type strains. Light intensity was dependent on growth conditions and biomass, being suitable to determine antifungal efficacy in vitro. This work describes for the first time the generation of reporter strains in a basal fungus that will allow real-time, non-invasive infection monitoring in insect and murine models, and the testing of antifungal efficacy by means other than survival.
Rediscovery of Red Wolf Ghost Alleles in a Canid Population Along the American Gulf Coast
Authors: Heppenheimer Elizabeth , Brzeski Kristin E. , Wooten Ron , Waddell William , Rutledge Linda Y. , Chamberlain Michael J. , Stahler Daniel R. , Hinton Joseph W. , VonHoldt Bridgett M.
Published: December 2018

Rediscovering species once thought to be extinct or on the edge of extinction is rare. Red wolves have been extinct along the American Gulf Coast since 1980, with their last populations found in coastal Louisiana and Texas. We report the rediscovery of red wolf ghost alleles in a canid population on Galveston Island, Texas. We analyzed over 7000 single nucleotide polymorphisms (SNPs) in 60 canid representatives from all legally recognized North American Canis species and two phenotypically ambiguous canids from Galveston Island. We found notably high Bayesian cluster assignments of the Galveston canids to captive red wolves with extensive sharing of red wolf private alleles. Today, the only known extant wild red wolves persist in a reintroduced population in North Carolina, which is dwindling amongst political and taxonomic controversy. Our rediscovery of red wolf ancestry after almost 40 years introduces both positive opportunities for additional conservation action and difficult policy challenges.
Permafrost-Landscape Map of the Republic of Sakha (Yakutia) on a Scale 1:1,500,000
Authors: Fedorov Alexander N. , Vasilyev Nikolay F. , Torgovkin Yaroslav I. , Shestakova Alena A. , Varlamov Stepan P. , Zheleznyak Mikhail N. , Shepelev Viktor V. , Konstantinov Pavel Y. , Kalinicheva Svetlana S. , Basharin Nikolay I. , Makarov Viktor S. , Ugarov Innokenty S. , Efremov Peter V. , Argunov Radomir N. , Egorova Larisa S. , Samsonova Vera V. , Shepelev Andrey G. , Vasiliev Alexander I. , Ivanova Rosaliya N. , Galanin Alexey A. , Lytkin Vasily M. , Kuzmin Georgy P. , Kunitsky Viktor V.
Published: December 2018

The history of permafrost landscape map compilation is related to the study of ecological problems with permafrost. Permafrost-landscape studies are now widely used in geocryological mapping. Permafrost-landscape classifications and mapping are necessary for studying the trends in development of the natural environment in northern and high-altitude permafrost regions. The cryogenic factor in the permafrost zone plays a leading role in the differentiation of landscapes, so it must be considered during classification construction. In this study, a map’s special content was developed using publications about Yakutian nature, archive sources from academic institutes, the interpretation of satellite images, and special field studies. Overlays of 20 types of terrain, identified by geological and geomorphological features, and 36 types of plant groupings, allowed the systematization of permafrost temperature and active layer thickness in 145 landscape units with relatively homogeneous permafrost-landscape conditions in the Sakha (Yakutia) Republic. This map serves as a basis for applied thematic maps related to the assessment and forecast of permafrost changes during climate warming and anthropogenic impacts.
Formation and Accumulation of Pore Methane Hydrates in Permafrost: Experimental Modeling
Authors: Chuvilin Evgeny , Davletshina Dinara
Published: December 2018

Favorable thermobaric conditions of hydrate formation and the significant accumulation of methane, ice, and actual data on the presence of gas hydrates in permafrost suggest the possibility of their formation in the pore space of frozen soils at negative temperatures. In addition, today there are several geological models that involve the formation of gas hydrate accumulations in permafrost. To confirm the literature data, the formation of gas hydrates in permafrost saturated with methane has been studied experimentally using natural artificially frozen in the laboratory sand and silt samples, on a specially designed system at temperatures from 0 to −8 °C. The experimental results confirm that pore methane hydrates can form in gas-bearing frozen soils. The kinetics of gas hydrate accumulation in frozen soils was investigated in terms of dependence on the temperature, excess pressure, initial ice content, salinity, and type of soil. The process of hydrate formation in soil samples in time with falling temperature from +2 °C to −8 °C slows down. The fraction of pore ice converted to hydrate increased as the gas pressure exceeded the equilibrium. The optimal ice saturation values (45−65%) at which hydrate accumulation in the porous media is highest were found. The hydrate accumulation is slower in finer-grained sediments and saline soils. The several geological models are presented to substantiate the processes of natural hydrate formation in permafrost at negative temperatures.
Influence of Load Frequency on Cohesive Soil Respond
Authors: Kucharczyk Krystian , Głuchowski Andrzej , Miturski Maciej , Sas Wojciech
Published: December 2018

The mechanism of cohesive soils response to cycling loading is less investigated compared to cohesionless soils. Multiple load-unload cycles cause significant changes in the structure of cohesive soils, which result in complex behaviour under the given load. The aim of the paper was to investigate and study the influence of load frequency on cohesive soil reaction. In order to obtain results, tests were conducted using the cyclic triaxial apparatus. Three cyclic tests were carried out, each for different frequency −0.5 Hz, 1.0 Hz, 2.0 Hz and one static triaxial test. The maximal value of deviator stress qmax, used in the cyclic tests, was set to 40 kPa. Afterwards samples were unloaded to qmin = 30 kPa. Cyclic loading triaxial tests were performed in a consolidated-undrained (CU) one-way loading manner, a sinusoidal waves were used. After the cycling loading was completed, a static triaxial shear test was conducted. Changes in the cohesive soil responses depending on cycling load frequency were presented in the paper. Differences in the accumulation of plastic strains were noticed, as well as changes of degradation index values, resilient degradation index values and differences in the excess pore water pressure development.
Repeated Heat Regeneration of Bone Char for Sustainable Use in Fluoride Removal from Drinking Water
Authors: Herath H. M. Ayala S. , Kawakami Tomonori , Tafu Masamoto
Published: December 2018

The effectiveness of regenerated chicken bone char (CBC) in fluoride removal was investigated in the present study. Heat treatment was studied as the regeneration method. Results revealed that the CBC regenerated at 673 K yielded the highest fluoride adsorption capacity, hence, 673 K was the best regenerating temperature. The study continued up to five regeneration cycles at the best regenerating temperature; 673 K. The CBC accounted to 16.1 mg F/g CBC as the total adsorption capacity after five regeneration cycles. The recovery percentage of CBC reduced from 79% at the first regeneration to 4% after five regeneration cycles. The hydroxyapatite structure of CBC was not changed during the fluoride adsorption by five regeneration cycles. The ion exchange incorporated with the chemical precipitation occurred during the fluoride adsorption. The repeated regeneration of CBC is possible and it could be used as a low cost defluoridation technique to minimize the wastage of bone char.
Future Implications of Using Registered Dietitians in Multidisciplinary Polycystic Ovary Syndrome Treatment
Authors: Wolf Wendy M. , Wattick Rachel A. , Murray Pamela J. , Clemmer Melanie , Olfert Melissa D.
Published: December 2018

Polycystic ovary syndrome (PCOS) is the most common reproductive endocrine disorder in females with insulin resistance playing a key role in pathogenesis. The objective of this study was to investigate current trends and future implications of multidisciplinary PCOS clinics with inclusion of dietitians. A two-phase, formative investigation on practitioners was conducted through an anonymous survey followed by focus groups. Survey respondents included 261 health care providers from around the world; the majority (59%) representing multidisciplinary teams. Focus group participants included four dietitians, three physicians, a health psychologist and a licensed nutritionist. Primary barriers for future multidisciplinary clinics included: money/resources, insurance reimbursement, and difference of opinions. Potential advantages included: more comprehensive and integrated care, greater convenience/efficiency, and better long-term outcomes. A majority of respondents (89%) stated that dietitians should be ‘involved’ or ‘highly involved’ in treatment. The greatest challenges for dietitians include insurance, limited disease knowledge, and lack of referrals. Most providers agreed that multidisciplinary clinics would lead to a better prognosis. A greater emphasis needs to be placed on educating professionals on the importance of nutrition counseling. Access to educated dietitians is likely the best way to ensure that PCOS patients have access to lifestyle interventions.
Ward-Based Non-Invasive Ventilation in Acute Exacerbations of COPD: A Narrative Review of Current Practice and Outcomes in the UK
Authors: Trethewey Samuel P. , Edgar Ross G. , Turner Alice M. , Mukherjee Rahul
Published: December 2018

Non-invasive ventilation (NIV) is frequently used as a treatment for acute hypercapnic respiratory failure (AHRF) in hospitalised patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). In the UK, many patients with AHRF secondary to AECOPD are treated with ward-based NIV, rather than being treated in critical care. NIV has been increasingly used as an alternative to invasive ventilation and as a ceiling of treatment in patients with a ‘do not intubate’ order. This narrative review describes the evidence base for ward-based NIV in the context of AECOPD and summarises current practice and clinical outcomes in the UK.
European Silver Sources from the 15th to the 17th Century: The Influx of “New World” Silver in Portuguese Currency
Authors: Borges Rui , Silva R. J. C. , Alves Luís C. , Araújo M. F. , Candeias António , Corregidor Victoria , Vieira João
Published: December 2018

The circulation trading routes and the characterization of the silver metal used in the European continent in the 15–17th centuries are historical issues that are still open. This study aimed to bring an insight into the silver processed within a chronological framework in the Portuguese territory, relating the analytical data with the known historical information. This investigation developed on 230 high silver coins from two important Portuguese coin collections was based mainly on surface particle-induced X-ray emission (PIXE) analysis, complemented with a few energy-dispersive X-ray fluorescence (EDXRF) analyses. The silver processed in different timelines was discriminated based on the variation of the impurity contents, namely Au and Bi. European silver with high Au and Hg and low Pb and Bi contents supplied the 15th century chronologies, being replaced at the dawn of the 16th century by a new metal entering the Portuguese capital. This new metal, with low Au and high Bi contents, was probably derived from European argentiferous copper ores. By the end of the 1500s, the Philippine chronologies reveal the newly discovered Potosí silver, identified for the first time based on PIXE minor and trace element surface contents, distinguishable from the European silver in use until 1578 in the Portuguese territory, by Au contents <100 ppm and very low Bi contents.
Spatiotemporal Variation and Hot Spot Detection of Visceral Leishmaniasis Disease in Kashi Prefecture, China
Authors: Zheng Canjun , Fu Jingying , Li Zeng , Lin Gang , Jiang Dong , Zhou Xiao-nong
Published: December 2018

Visceral leishmaniasis (VL) remains a serious public health problem in China. To explore the temporal, spatial, and spatiotemporal characteristics of visceral leishmaniasis (VL), the spatial and spatiotemporal clustering distribution and their relationships with the surrounding geographic environmental factors were analyzed. In this study, the average nearest-neighbor distance (ANN), Ripley’s K-function and Moran’s I statistics were used to evaluate spatial autocorrelation in the VL distribution of the existing case patterns. Getis–Ord Gi* was used to identify the hot-spot and cold-spot areas based on Geographic Information System (GIS), and spatiotemporal retrospective permutation scan statistics was used to detect the spatiotemporal clusters. The results indicated that VL continues to be a serious public health problem in Kashi Prefecture, China, particularly in the north-central region of Jiashi County, which is a relatively high-risk area in which hot spots are distributed. Autumn and winter months were the outbreak season for VL cases. The detection of spatial and spatiotemporal patterns can provide epidemiologists and local governments with significant information for prevention measures and control strategies.
Spatial Patterns of Satellite-Retrieved PM2.5 and Long-Term Exposure Assessment of China from 1998 to 2016
Authors: Chen Tan , Deng Shulin , Li Manchun
Published: December 2018

Previous studies have shown that particulate matter with an aerodynamic diameter of less than 2.5 micrometers (PM2.5) is tightly associated with adverse effects on human health, i.e., morbidity and mortality. Based on long-term satellite-derived PM2.5 datasets, this study analyzed the spatial patterns and temporal trends of PM2.5 concentrations in China from 1998 to 2016 using standard deviational ellipse and statistical analyses. A long-term assessment of exposure and health impacts due to PM2.5 was undertaken by the Environmental Benefits Mapping and Analysis Program-Community Edition (BenMAP-CE) model. The results show that concentrations of PM2.5 increased nonlinearly in most areas of China from 1998 to 2016. Higher concentrations were found in eastern China and western Tarim Basin, and most areas exceeded the World Health Organization’s (WHO) annual PM2.5 standards. The median center of average PM2.5 concentration of the country shifted to the southeast and then returned during the examined time period. The proportion of the population exposed to equal PM2.5 concentrations increased at first, then trended downward. The proportion of the population exposed to PM2.5 over WHO Interim Target-1 (35 µg/m3) increased 20.6%, which was the largest growth compared with other WHO standard levels. The extent of health risk in China increased and expanded from 1998 to 2016, especially in the Beijing-Tianjin-Hebei region, the Yangtze River Delta, and the Pearl River Delta, which are China’s top three urban areas. The implementation of the Air Pollution Prevention and Control Action Plan has gradually paid off. If the government can achieve long-term adherence to its plan, great economic and health benefits will be gotten through the BenMAP-CE model analysis.
The Effects of Mind-Body Exercise on Cognitive Performance in Elderly: A Systematic Review and Meta-Analysis
Authors: Zhang Yanjie , Li Chunxiao , Zou Liye , Liu Xiaolei , Song Wook
Published: December 2018

Background: As the situation of cognitive aging is getting worse, preventing or treating cognitive decline through effective strategies is highly important. This systematic review aims to investigate whether mind-body exercise is an effective approach for treating cognition decline. Methods: Searches for the potential studies were performed on the eight electronic databases (MEDLINE, Scopus, Web of Science, SPORTDiscus, CINAHL, PsycArtilces, CNKI, and Wanfang). Randomized controlled trials (RCTs) examining the effect of mind-body exercise on cognitive performance in older adults were included. Data were extracted and effect sizes were pooled with 95% confidence intervals (95% CI) using random-effects models. The Physiotherapy Evidence Database Scale was employed to examine the study quality. Results: Nineteen RCTs including 2539 elders (67.3% female) with fair to good study quality were identified. Mind-body exercise, relative to control intervention, showed significant benefits on cognitive performance, global cognition (Hedges’g = 0.23), executive functions (Hedges’g = 0.25 to 0.65), learning and memory (Hedges’g = 0.37 to 0.49), and language (Hedges’g = 0.35). In addition, no significant adverse events were reported. Conclusion: Mind-body exercise may be a safe and effective intervention for enhancing cognitive function among people aged 60 years or older. Further research evidence is still needed to make a more conclusive statement.
Pharmacological and Non-Pharmacological Methods of Labour Pain Relief—Establishment of Effectiveness and Comparison
Authors: Czech Iwona , Fuchs Piotr , Fuchs Anna , Lorek Miłosz , Tobolska-Lorek Dominika , Drosdzol-Cop Agnieszka , Sikora Jerzy
Published: December 2018

Background: To evaluate the effectiveness of pharmacological and non-pharmacological pain relief methods and to compare them. Materials and methods: 258 women were included in the study and interviewed using a questionnaire and the visual analogue scale for pain. They were divided into six groups depending on chosen method of labour pain relief: epidural anaesthesia (EA; n = 42), water immersion and water birth (WB; n = 40), nitrous oxide gas for pain control (G; n = 40), transcutaneous electrical nerve stimulation (TENS) (n = 50), multiple management (MM; n = 42), none (N; n = 44). Results: The average age of the women was 29.4 ± 3.74 years and 60.47% of them were nulliparous (n = 156). Mean values of labour pain intensity were 6.81 ± 2.26 during the first stage of labour; 7.86 ± 2.06 during the second stage, and 3.22 ± 2.46 during the third stage. There was no significant difference in pain level between epidural analgesia and gas groups in the first stage of labour (p = 0.74). Nevertheless, epidural analgesia reduced pain level during the second and third stage (both p < 0.01). The highest satisfaction level pertains to water immersion (n = 38; 95%). Conclusion: Epidural analgesia is the gold standard of labour pain relief, however water birth was found to be associated with the highest satisfaction level of the parturient women. The contentment of childbirth depends not only on the level of experienced pain, but also on the care provided to the parturient during pregnancy and labour.
An Optimization Model for a Wetland Restoration Project under Uncertainty
Authors: Cai Baofeng , Zhang Yang , Wang Xianen , Li Yu
Published: December 2018

Restoring natural wetlands with conservation projects is an urgent task for human well-being. This paper introduces the Interval linear programming (ILP) method in wetland restoration projects for the first time and builds an optimization model. The purpose of the optimization model is to find an optimal restoration measures allocation pattern that can minimize the total investment in wetland restoration projects and obtain additional ecological environment and socio-economic benefits. The optimization model can also decrease the influence of interval uncertainty in the system by expressing the executed solution as interval numbers with an upper bound and a lower bound. The result of the optimization model for the wetland restoration project indicated a range of 6.84%–15.43% reduction on comparison with the original scheme which verified the effectiveness and validity of this optimization model. Our findings indicate that higher ecological and social benefits of wetland restoration projects can be achieved with lower restoration investment on the application of the reasonable and optimal restoration measures allocation pattern by the optimization model. The results of interval solutions can provide guidance for project managers to select a satisfactory decision-making plan by adjusting the decision variables in the interval solutions according to the practical situation. It can be seen that reeds were suggested to be planted over 46.75 km2, with the same lower bound and higher bound. Meanwhile, populus euphratica, and dryland willow were recommended to be planted in a mixed forest pattern within the interval of 30.54 km2 to 37.25 km2, and so forth. With the optimal solutions obtained from the model, the total project investment would be in the range of 2193.14 (104 CNY) to 2416.01 (104 CNY). Future improvements of our optimization model in wetland restoration projects should consider other kinds of uncertainties in the system such as stochastic uncertainties, fuzzy uncertainties, and integrated uncertainties.
Artificial Intelligence and Big Data in Public Health
Authors: Benke Kurt , Benke Geza
Published: December 2018

Artificial intelligence and automation are topics dominating global discussions on the future of professional employment, societal change, and economic performance. In this paper, we describe fundamental concepts underlying AI and Big Data and their significance to public health. We highlight issues involved and describe the potential impacts and challenges to medical professionals and diagnosticians. The possible benefits of advanced data analytics and machine learning are described in the context of recently reported research. Problems are identified and discussed with respect to ethical issues and the future roles of professionals and specialists in the age of artificial intelligence.
Profile Resemblance in Health-Related Markers: The Portuguese Sibling Study on Growth, Fitness, Lifestyle, and Health
Authors: Pereira Sara , Katzmarzyk Peter T. , Hedeker Donald , Maia José
Published: December 2018

The co-occurrence of health-related markers and their associations with individual, family, and environmental characteristics have not yet been widely explored in siblings. We aimed to identify multivariate profiles of health-related markers, analyze their associations with biological, sociodemographic, and built environment characteristics, and estimate sibling resemblance in these profiles. The sample includes 736 biological siblings aged 9–20 years. Body fat was measured with a portable bioelectrical impedance scale; biological maturation was assessed with the maturity offset; handgrip strength, standing long jump, one-mile run, and shuttle run were used to mark physical fitness. Health behaviors, sociodemographic, and built environmental characteristics were recorded by questionnaire. Latent profile analysis and multilevel logistic regression models were used; sibling resemblance was estimated with the intraclass correlation (ρ). Two multivariate profiles emerged: “P1 = fit, lower fat and poorer diet” (86.7%) and “P2 = higher fat and lower fit, but better diet” (13.3%). Siblings whose fathers were less qualified in their occupation were more likely to belong to P2 (OR = 1.24, p = 0.04); those whose fathers with Grade 12 and university level education were more likely to fit in P2 compared to peers living with fathers having an educational level below Grade 12 (OR = 3.18, p = 0.03, and OR = 6.40, p = 0.02, Grade 12 and university level, respectively). A moderate sibling profile resemblance was found (0.46 ≤ ρ ≤ 0.55). In conclusion, youth health-related markers present substantial differences linked with their body composition, physical fitness and unhealthy diet. Furthermore, only father socio-demographic characteristics were associated with profile membership. Sibling´s profile resemblance mirrors the effects of genetics and shared characteristics.
Air Pollution Exposure in Walking School Bus Routes: A New Zealand Case Study
Authors: Dirks Kim N. , Salmond Jennifer A. , Talbot Nicholas
Published: December 2018

Walking School Buses (WSBs), organized groups for children to walk to school under the supervision of adults, help reduce traffic congestion and contribute towards exercise. Routes are based largely on need, traffic safety and travel time, with exposure to air pollution not generally considered. This paper explores whether reductions in exposure can be achieved based on the side of the road travelled using data collected in Auckland, New Zealand. Exposure to air pollution was measured for a 25-min commute consisting of a 10-min segment along a quiet cul-de-sac and a 15-min segment along a main arterial road with traffic congestion heavier in one direction. Two participants were each equipped with a portable P-Trak ultrafine particle monitor and a portable Langan carbon monoxide monitor, and walked the route on opposite sides of the road simultaneously, for both morning and afternoon, logging 10-s data. The results suggest that pedestrians travelling on the footpath next to the less congested side of the road in the morning avoid many short-term peaks in concentration and experience significantly lower mean exposures than those travelling on the footpath next to the more congested side. Significant reductions in air pollution exposure could be made for children by taking into account the side of the road in WSB route design.
Risk Factors for Abdominal Aortic Aneurysm in Population-Based Studies: A Systematic Review and Meta-Analysis
Authors: Altobelli Emma , Rapacchietta Leonardo , Profeta Valerio F. , Fagnano Roberto
Published: December 2018

Abdominal aortic aneurysm (AAA) represents an important public health problem with a prevalence between 1.3% and 12.5%. Several population-based randomized trials have evaluated ultrasound screening for AAA providing evidence of a reduction in aneurysm-related mortality in the screened population. The aim of our study was to perform a systematic review and meta-analysis of the risk factors for AAA. We conducted a systematic review of observational studies and we performed a meta-analysis that evaluated the following risk factors: gender, smoking habits, hypertension, coronary artery disease and family history of AAA. Respect to a previous a meta-analysis we added the funnel plot to examine the effect sizes estimated from individual studies as measure of their precision; sensitivity analysis to check the stability of study findings and estimate how the overall effect size would be modified by removal of one study; cumulative analysis to evaluate the trend between studies in relation to publication year. Abdominal aortic aneurysm prevalence is higher in smokers and in males. On the other hand, while diabetes is a risk factor for many cardiovascular diseases, it is not a risk factor for AAA. In addition, it is important to underline that all countries, where AAA screening was set up, had high income level and the majority belong to Western Europe (United Kingdom, Sweden, Italy, Poland, Spain and Belgium). Abdominal aortic aneurysm screening is fundamental for public health. It could avoid deaths, ruptures, and emergency surgical interventions if abdominal aortic aneurysm was diagnosed early in the population target for screening.
Who Is Serving Us? Food Safety Rules Compliance Among Brazilian Food Truck Vendors
Authors: Auad Lígia Isoni , Ginani Verônica Cortez , Dos Santos Leandro Eliana , Nunes Aline Costa Santos , Domingues Junior Luiz Roberto Pires , Zandonadi Renata Puppin
Published: December 2018

The rise of food trucks as an eating out option requires knowledge of this sector. Balancing the reality of the food truck sector with access to safe food should guide actions and public policies to cater to its peculiarities. Therefore, this study aimed to characterize the Brazilian food truck vendors’ profile regarding their socioeconomic status and compliance with food safety rules. From the 118 food truck vendors registered in the Brazilian Federal District, 30% (n = 35) participated in the study. We conducted structured interviews from December 2017 to April 2018. We ranked compliance levels according to a five-point Likert scale based on calculated compliance scores. The interviews revealed that food truck vendors were mostly married males, who had completed at least a tertiary education level, and wanted to start up their own businesses. The compliance levels depict good compliance with food safety rules (overall compliance (OC)-score = 0.69, on a 0 to 1 scale). The food trucks assessed in this study distinguished themselves from the street food and food retail sectors due to their operational structure and the complexity of food production processes. Those particular features should be considered to ensure adequate and effective sanitary control and inspections, as well as to reduce the probability of microbial growth and food contamination and the consequent risk of foodborne illnesses.
Process Mining and Conformance Checking of Long Running Processes in the Context of Melanoma Surveillance
Authors: Rinner Christoph , Helm Emmanuel , Dunkl Reinhold , Kittler Harald , Rinderle-Ma Stefanie
Published: December 2018

Background: Process mining is a relatively new discipline that helps to discover and analyze actual process executions based on log data. In this paper we apply conformance checking techniques to the process of surveillance of melanoma patients. This process consists of recurring events with time constraints between the events. Objectives: The goal of this work is to show how existing clinical data collected during melanoma surveillance can be prepared and pre-processed to be reused for process mining. Methods: We describe an approach based on time boxing to create process models from medical guidelines and the corresponding event logs from clinical data of patient visits. Results: Event logs were extracted for 1023 patients starting melanoma surveillance at the Department of Dermatology at the Medical University of Vienna between January 2010 and June 2017. Conformance checking techniques available in the ProM framework and explorative applied process mining techniques were applied. Conclusions: The presented time boxing enables the direct use of existing process mining frameworks like ProM to perform process-oriented analysis also with respect to time constraints between events.
Smartphone Applications for Mindfulness Interventions with Suicidality in Asian Older Adults: A Literature Review
Authors: Choo Carol C. , Kuek Jonathan H. L. , Burton André A. D.
Published: December 2018

Elderly suicide is a rising concern. Despite the advent of mobile technology, there remained a gap in the evidence base as to whether smartphone applications could be used for mindfulness intervention for suicidality in Asian older adults. This paper aimed to review recent research relevant to smartphone applications that could be used in providing mindfulness interventions for suicidality to Asian older adults. The inclusion criteria for this review were papers published in peer-reviewed journals from 2008 to 2018 with the usage of specific search terms, namely, ‘smartphone application’, ‘mobile application’, and ‘mindfulness’, assessed against the inclusion criteria and screened by an experienced Asian clinician to be of clinical utility for mindfulness intervention for suicidality with Asian older adults. Initial search on databases yielded 236 results. A total of 35 full text papers that fit the inclusion criteria were assessed for eligibility and 10 papers were included in the current review. This review highlighted the paucity of rigorous empirically validated research into effective smartphone applications that can be used for mindfulness interventions for suicidality with Asian older adults.
Impact of Air Pollution on Sedentary Behavior: A Cohort Study of Freshmen at a University in Beijing, China
Authors: Yu Hongjun , Cheng Jiali , Gordon Shelby Paige , An Ruopeng , Yu Miao , Chen Xiaodan , Yue Qingli , Qiu Jun
Published: December 2018

Human populations worldwide have experienced substantial environmental issues in part due to air pollution, notably in China. Gaps in the scientific literature remain regarding the relationship between air pollution and sedentary behavior among young adults in China. The purpose of this study is to examine the effect of air pollution on sedentary behavior among college students living in Beijing, China. We conducted follow-up health surveys on 12,174 freshman students enrolled at Tsinghua University from 2013 to 2017. Sedentary behavior was measured using the short version of the International Physical Activity Questionnaire (IPAQ). Corresponding air pollution data measured by the Ministry of Environmental Protection of the People’s Republic of China were collected to include the average hourly air quality index (AQI), PM2.5, PM10, and NO2 (µg/m³). The data were analyzed using linear individual fixed-effect regressions. An increase in air pollution concentration of one standard deviation in AQI, PM2.5, PM10, and NO2 was associated with an increase in weekly total hours of sedentary behavior by 7.35 (95% confidence interval (CI) = 5.89, 8.80), 6.24 (95% CI = 5.00, 7.49), 6.80 (95% CI = 5.46, 8.15), and 7.06 (95% CI = 5.65, 8.47), respectively. In the presence of air pollution, women students tended to increase their sedentary behavior more than men. Air pollution increases sedentary behavior among freshman students living in Beijing, China. Replication of this study is warranted among various populations within China.
A Parallel-Computing Approach for Vector Road-Network Matching Using GPU Architecture
Authors: Wan Bo , Yang Lin , Zhou Shunping , Wang Run , Wang Dezhi , Zhen Wenjie
Published: December 2018

The road-network matching method is an effective tool for map integration, fusion, and update. Due to the complexity of road networks in the real world, matching methods often contain a series of complicated processes to identify homonymous roads and deal with their intricate relationship. However, traditional road-network matching algorithms, which are mainly central processing unit (CPU)-based approaches, may have performance bottleneck problems when facing big data. We developed a particle-swarm optimization (PSO)-based parallel road-network matching method on graphics-processing unit (GPU). Based on the characteristics of the two main stages (similarity computation and matching-relationship identification), data-partition and task-partition strategies were utilized, respectively, to fully use GPU threads. Experiments were conducted on datasets with 14 different scales. Results indicate that the parallel PSO-based matching algorithm (PSOM) could correctly identify most matching relationships with an average accuracy of 84.44%, which was at the same level as the accuracy of a benchmark—the probability-relaxation-matching (PRM) method. The PSOM approach significantly reduced the road-network matching time in dealing with large amounts of data in comparison with the PRM method. This paper provides a common parallel algorithm framework for road-network matching algorithms and contributes to integration and update of large-scale road-networks.
Nutrient-Responsive Small Signaling Peptides and Their Influence on the Root System Architecture
Authors: Lay Katerina S. , Takahashi Hideki
Published: December 2018

The root system architecture (RSA) of plants is highly dependent on the surrounding nutrient environment. The uptake of essential nutrients triggers various signaling cascades and fluctuations in plant hormones to elicit physical changes in RSA. These pathways may involve signaling components known as small signaling peptides (SSPs), which have been implicated in a variety of plant developmental processes. This review discusses known nutrient-responsive SSPs with a focus on several subclasses that have been shown to play roles in root development. Most functionally well-characterized cases of SSP-mediated changes in RSA are found in responses to nitrogen (N) and phosphorus (P) availability, but other nutrients have also been known to affect the expression of SSP-encoding genes. These nutrient-responsive SSPs may interact downstream with leucine-rich repeat receptor kinases (LRR-RKs) to modulate hormone signaling and cellular processes impacting plant root development. SSPs responsive to multiple nutrient cues potentially act as mediators of crosstalk between the signaling pathways. Study of SSP pathways is complicated because of functional redundancy within peptide and receptor families and due to their functionality partly associated with post-translational modifications; however, as genomic research and techniques progress, novel SSP-encoding genes have been identified in many plant species. Understanding and characterizing the roles of SSPs influencing the root phenotypes will help elucidate the processes that plants use to optimize nutrient acquisition in the environment.
The Power of Fish Models to Elucidate Skin Cancer Pathogenesis and Impact the Discovery of New Therapeutic Opportunities
Authors: Sarasamma Sreeja , Lai Yu-Heng , Liang Sung-Tzu , Liu Kechun , Hsiao Chung-Der
Published: December 2018

Animal models play important roles in investigating the pathobiology of cancer, identifying relevant pathways, and developing novel therapeutic tools. Despite rapid progress in the understanding of disease mechanisms and technological advancement in drug discovery, negative trial outcomes are the most frequent incidences during a Phase III trial. Skin cancer is a potential life-threatening disease in humans and might be medically futile when tumors metastasize. This explains the low success rate of melanoma therapy amongst other malignancies. In the past decades, a number of skin cancer models in fish that showed a parallel development to the disease in humans have provided important insights into the fundamental biology of skin cancer and future treatment methods. With the diversity and breadth of advanced molecular genetic tools available in fish biology, fish skin cancer models will continue to be refined and expanded to keep pace with the rapid development of skin cancer research. This review begins with a brief introduction of molecular characteristics of skin cancers, followed by an overview of teleost models that have been used in the last decades in melanoma research. Next, we will detail the importance of the zebrafish (Danio rerio) animal model and other emerging fish models including platyfish (Xiphophorus sp.), and medaka (Oryzias latipes) in future cutaneous malignancy studies. The last part of this review provides the recent development and genome editing applications of skin cancer models in zebrafish and the progress in small molecule screening.
Root Abscisic Acid Contributes to Defending Photoinibition in Jerusalem Artichoke (Helianthus tuberosus L.) under Salt Stress
Authors: Yan Kun , Bian Tiantian , He Wenjun , Han Guangxuan , Lv Mengxue , Guo Mingzhu , Lu Ming
Published: December 2018

The aim of the study was to examine the role of root abscisic acid (ABA) in protecting photosystems and photosynthesis in Jerusalem artichoke against salt stress. Potted plants were pretreated by a specific ABA synthesis inhibitor sodium tungstate and then subjected to salt stress (150 mM NaCl). Tungstate did not directly affect root ABA content and photosynthetic parameters, whereas it inhibited root ABA accumulation and induced a greater decrease in photosynthetic rate under salt stress. The maximal photochemical efficiency of PSII (Fv/Fm) significantly declined in tungstate-pretreated plants under salt stress, suggesting photosystem II (PSII) photoinhibition appeared. PSII photoinhibition did not prevent PSI photoinhibition by restricting electron donation, as the maximal photochemical efficiency of PSI (ΔMR/MR0) was lowered. In line with photoinhibition, elevated H2O2 concentration and lipid peroxidation corroborated salt-induced oxidative stress in tungstate-pretreated plants. Less decrease in ΔMR/MR0 and Fv/Fm indicated that PSII and PSI in non-pretreated plants could maintain better performance than tungstate-pretreated plants under salt stress. Consistently, greater reduction in PSII and PSI reaction center protein abundance confirmed the elevated vulnerability of photosystems to salt stress in tungstate-pretreated plants. Overall, the root ABA signal participated in defending the photosystem’s photoinhibition and protecting photosynthesis in Jerusalem artichoke under salt stress.
Rice Overexpressing OsNUC1-S Reveals Differential Gene Expression Leading to Yield Loss Reduction after Salt Stress at the Booting Stage
Authors: Boonchai Chuthamas , Udomchalothorn Thanikarn , Sripinyowanich Siriporn , Comai Luca , Buaboocha Teerapong , Chadchawan Supachitra
Published: December 2018

Rice nucleolin (OsNUC1), consisting of two isoforms, OsNUC1-L and OsNUC1-S, is a multifunctional protein involved in salt-stress tolerance. Here, OsNUC1-S’s function was investigated using transgenic rice lines overexpressing OsNUC1-S. Under non-stress conditions, the transgenic lines showed a lower yield, but higher net photosynthesis rates, stomatal conductance, and transpiration rates than wild type only in the second leaves, while in the flag leaves, these parameters were similar among the lines. However, under salt-stress conditions at the booting stage, the higher yields in transgenic lines were detected. Moreover, the gas exchange parameters of the transgenic lines were higher in both flag and second leaves, suggesting a role for OsNUC1-S overexpression in photosynthesis adaptation under salt-stress conditions. Moreover, the overexpression lines could maintain light-saturation points under salt-stress conditions, while a decrease in the light-saturation point owing to salt stress was found in wild type. Based on a transcriptome comparison between wild type and a transgenic line, after 3 and 9 days of salt stress, the significantly differentially expressed genes were enriched in the metabolic process of nucleic acid and macromolecule, photosynthesis, water transport, and cellular homeostasis processes, leading to the better performance of photosynthetic processes under salt-stress conditions at the booting stage.
Altered Purinergic Receptor Sensitivity in Type 2 Diabetes-Associated Endothelial Dysfunction and Up4A-Mediated Vascular Contraction
Authors: Mahdi Ali , Jiao Tong , Tratsiakovich Yahor , Yang Jiangning , Östenson Claes-Göran , Pernow John , Zhou Zhichao
Published: December 2018

Purinergic signaling may be altered in diabetes accounting for endothelial dysfunction. Uridine adenosine tetraphosphate (Up4A), a novel dinucleotide substance, regulates vascular function via both purinergic P1 and P2 receptors (PR). Up4A enhances vascular contraction in isolated arteries of diabetic rats likely through P2R. However, the precise involvement of PRs in endothelial dysfunction and the vasoconstrictor response to Up4A in diabetes has not been fully elucidated. We tested whether inhibition of PRs improved endothelial function and attenuated Up4A-mediated vascular contraction using both aortas and mesenteric arteries of type 2 diabetic (T2D) Goto Kakizaki (GK) rats vs. control Wistar (WT) rats. Endothelium-dependent (EDR) but not endothelium-independent relaxation was significantly impaired in both aortas and mesenteric arteries from GK vs. WT rats. Non-selective inhibition of P1R or P2R significantly improved EDR in aortas but not mesenteric arteries from GK rats. Inhibition of A1R, P2X7R, or P2Y6R significantly improved EDR in aortas. Vasoconstrictor response to Up4A was enhanced in aortas but not mesenteric arteries of GK vs. WT rats via involvement of A1R and P2X7R but not P2Y6R. Depletion of major endothelial component nitric oxide enhanced Up4A-induced aortic contraction to a similar extent between WT and GK rats. No significant differences in protein levels of A1R, P2X7R, and P2Y6R in aortas from GK and WT rats were observed. These data suggest that altered PR sensitivity accounts for endothelial dysfunction in aortas in diabetes. Modulating PRs may represent a potential therapy for improving endothelial function.