bluuebunny/crossref_metadata_2025 huggingface dataset:
Automatic identification and classification of compostable and biodegradable plastics using hyperspectral imaging
Authors: Taneepanichskul Nutcha , Hailes Helen C. , Miodownik Mark
Published: March 2023

In the UK waste management systems biodegradable and compostable packaging are not automatically detected and separated. As a result, their fate is generally landfill or incineration, neither of which is an environmentally good outcome. Thus, effective sorting technologies for compostable plastics are needed to help improve composting rates of these materials and reduce the contamination of recycling waste streams. Hyperspectral imaging (HSI) was applied in this study to develop classification models for automatically identifying and classifying compostable plastics with the analysis focused on the spectral region 950–1,730 nm. The experimental design includes a hyperspectral imaging camera, allowing different chemometric techniques to be applied including principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) to develop a classification model for the compostable materials plastics. Materials used in this experimental analysis included compostable materials (sugarcane-derived and palm leaf derived), compostable plastics (PLA, PBAT) and conventional plastics (PP, PET, and LDPE). Our strategy was to develop a classification model to identify and categorize various fragments over the size range of 50 x 50 mm to 5 x 5 mm. Results indicated that both PCA and PLS-DA achieved classification scores of 100% when the size of material was larger than 10 mm x 10 mm. However, the misclassification rate increased to 20% for sugarcane-derived and 40% for palm leaf-based materials at sizes of 10 x 10 mm or below. In addition, for sizes of 5 x 5 mm, the misclassification rate for LDPE and PBAT increased to 20%, and for sugarcane and palm-leaf based materials to 60 and 80% respectively while the misclassification rate for PLA, PP, and PET was still 0%. The system is capable of accurately sorting compostable plastics (compostable spoons, forks, coffee lids) and differentiating them from identical looking conventional plastic items with high accuracy.
Mechanisms to change farmers' drought adaptation behaviors in Sistan and Baluchistan Province, Iran
Authors: Khoshnodifar Zahra , Karimi Hamid , Ataei Pouria
Published: March 2023

Introduction

Frequent droughts in Iran have imposed economic and social losses on farmers. To mitigate drought implications, farmers' behaviors should be shifted towards drought adaptation. This research mainly aimed to study mechanisms for changing farmers' drought adaptation behaviors in Sistan and Baluchistan Province.

Methods

The research was conducted among farmers in this province (N = 950). The sample whose size was estimated at 275 farmers by Krejcie and Morgan's table was taken by the stratified random sampling technique. The research instrument was a researcher-made questionnaire. Data were analyzed by SPSS23 and AMOS23 software. The comprehensive action determination model was validated by confirmatory factor analysis. Furthermore, structural equation modeling was used to explore the components influencing farmers' drought adaptation behaviors.

Results and discussion

According to the results, personal norms, attitudes, objective barriers, and subjective barriers had positive and significant effects on the farmers' intention to apply drought adaptation strategies. As well, the farmers' adaptation behaviors were influenced by their behavioral intentions, objective barriers, and subjective barriers. It can be concluded that changing the mechanism of farmers' behaviors toward drought adaptation is shaped by the processes included in the comprehensive action determination model in which norms, habits, situational influences, and intentional processes are involved.
Retrospective comparison of side effects and outcomes of three-dimensional conformal vs. intensity-modulated radiation therapy in the palliative-intent treatment (4 Gy × 5 daily fractions) of canine intranasal tumors (2010–2017)
Authors: Fu Dah-Renn , Weng Hsin-Yi , Mendez Valenzuela Carlos Roberto , Vanhaezebrouck Isabelle F. , Plantenga Jeannie M.
Published: March 2023

Objective

To compare the occurrence of radiation side effects and treatment outcomes in dogs with intranasal tumors treated with a total dose of 20 Gy delivered in 5 daily 4 Gy fractions using computer-based 3D conformal (3DCRT) or intensity-modulated (IMRT) radiation therapy plans.

Design

Retrospective case series.

Materials and methods

Medical records for dogs with intranasal tumors treated with 4 Gy × 5 fractions between 2010 and 2017 were reviewed. Radiation side effects, time to local progression (TTLP), progression-free survival (PFS) and survival time (OS) were evaluated.

Results

Thirty-six dogs (24 carcinomas, 10 sarcomas and 2 others) met the inclusion criteria. Sixteen were treated with 3DCRT and 20 with IMRT. Clinical signs improvement or resolution were reported in 84% of dogs. The median time to clinical signs improvement was 12 days (1–88 days) after the end of treatment. Eight dogs treated with 3DCRT (8/16, 50%) and 5 with IMRT (5/20, 25%) were documented acute radiation side effects. Almost all were classified as grade 1 skin, oral or ocular acute side effects. Only one dog in 3DCRT group was demonstrated grade 2 skin acute effects. The median TTLP for dogs treated with 3DCRT or IMRT was 238 days and 179 days, respectively (p= 0.967). The median PFS for 3DCRT or IMRT was 228 days and 175 days, respectively (p= 0.940). The median OS for 3DCRT or IMRT was 295 days and 312 days, respectively (p= 0.787). No significantly differences were observed in side effects, TTLP, PFS and OS between 3DCRT and IMRT groups.

Conclusions

Palliative-intent conformal radiation therapy given in five daily 4 Gy fractions relieved clinical signs with minimal radiation side effects, with no statistical difference in occurrence between 3DCRT and IMRT dogs.
Learning Distributed Representations and Deep Embedded Clustering of Texts
Authors: Wang Shuang , Beheshti Amin , Wang Yufei , Lu Jianchao , Sheng Quan Z. , Elbourn Stephen , Alinejad-Rokny Hamid
Published: March 2023

Instructors face significant time and effort constraints when grading students’ assessments on a large scale. Clustering similar assessments is a unique and effective technique that has the potential to significantly reduce the workload of instructors in online and large-scale learning environments. By grouping together similar assessments, marking one assessment in a cluster can be scaled to other similar assessments, allowing for a more efficient and streamlined grading process. To address this issue, this paper focuses on text assessments and proposes a method for reducing the workload of instructors by clustering similar assessments. The proposed method involves the use of distributed representation to transform texts into vectors, and contrastive learning to improve the representation that distinguishes the differences among similar texts. The paper presents a general framework for clustering similar texts that includes label representation, K-means, and self-organization map algorithms, with the objective of improving clustering performance using Accuracy (ACC) and Normalized Mutual Information (NMI) metrics. The proposed framework is evaluated experimentally using two real datasets. The results show that self-organization maps and K-means algorithms with Pre-trained language models outperform label representation algorithms for different datasets.
Differences between Monolinguals and Bilinguals in Phonetic and Phonological Learning and the Connection with Auditory Sensory Memory
Authors: Spinu Laura , Hwang Jiwon , Vasilita Mariana
Published: March 2023

Bilingualism has been linked with improved function regarding certain aspects of linguistic processing, e.g., novel word acquisition and learning unfamiliar sound patterns. Two non mutually-exclusive approaches might explain these results. One is related to executive function, speculating that more effective learning is achieved through actively choosing relevant information while inhibiting potentially interfering information. While still controversial, executive function enhancements attributed to bilingual experience have been reported for decades. The other approach, understudied to date, emphasizes the role of sensory mechanisms, specifically auditory sensory memory. Bilinguals outperformed monolinguals in tasks involving auditory processing and episodic memory recall, but the questions whether (1) bilinguals’ auditory sensory memory skills are also enhanced, and (2) phonetic skill and auditory sensory memory are correlated, remain open, however. Our study is innovative in investigating phonetic learning skills and auditory sensory memory in the same speakers from two groups: monolinguals and early bilinguals. The participants were trained and tested on an artificial accent of English and their auditory sensory memory was assessed based on a digit span task. The results demonstrated that, compared to monolinguals, bilinguals exhibit enhanced auditory sensory memory and phonetic and phonological learning skill, and a correlation exists between them.
Risk Factors of Complications from Central Bisectionectomy (H458) for Hepatocellular Carcinoma: A Multi-Institutional Single-Arm Analysis
Authors: Nanashima Atsushi , Eguchi Susumu , Hisaka Toru , Kawasaki Yota , Yamashita Yo-ichi , Ide Takao , Kuroki Tamotsu , Yoshizumi Tomoharu , Kitahara Kenji , Endo Yuichi , Utsunomiya Tohru , Kajiwara Masatoshi , Sakoda Masahiko , Okamoto Kohji , Nagano Hiroaki , Takami Yuko , Beppu Toru
Published: March 2023

Background: This study aims to clarify the perioperative risk factors and short-term prognosis of central bisectionectomy (CB) for hepatocellular carcinoma (HCC). Methods: Surgical data from 142 selected patients out of 171 HCC patients who underwent anatomical CB (H458) between 2005 and 2020 were collected from 17 expert institutions in a single-arm retrospective study. Results: Morbidities recorded by the International Study Group of Liver Surgery (ISGLS) from grade BC post-hepatectomy liver failure (PHLF) and bile leakage (PHBL), or complications requiring intervention were observed in 37% of patients. A multivariate analysis showed that increased blood loss (iBL) > 1500 mL from PHLF (risk ratio [RR]: 2.79), albumin level < 4 g/dL for PHBL (RR, 2.99), involvement of segment 1, a large size > 6 cm, or compression of the hepatic venous confluence or cava by HCC for all severe complications (RR: 5.67, 3.75, 6.51, and 8.95, respectively) (p < 0.05) were significant parameters. Four patients (3%) died from PHLF. HCC recurred in 50% of 138 surviving patients. The three-year recurrence-free and overall survival rates were 48% and 81%, respectively. Conclusions: Large tumor size and surrounding tumor involvement, or compression of major vasculatures and the related iBL > 1500 mL were independent risk factors for severe morbidities in patients with HCC undergoing CB.
Overview of Trop-2 in Cancer: From Pre-Clinical Studies to Future Directions in Clinical Settings
Authors: Lombardi Pasquale , Filetti Marco , Falcone Rosa , Altamura Valeria , Paroni Sterbini Francesco , Bria Emilio , Fabi Alessandra , Giannarelli Diana , Scambia Giovanni , Daniele Gennaro
Published: March 2023

Trophoblast cell surface antigen-2 (Trop-2) is a glycoprotein that was first described as a membrane marker of trophoblast cells and was associated with regenerative abilities. Trop-2 overexpression was also described in several tumour types. Nevertheless, the therapeutic potential of Trop-2 was widely recognized and clinical studies with drug–antibody conjugates have been initiated in various cancer types. Recently, these efforts have been rewarded with the approval of sacituzumab govitecan from both the Food and Drug Administration (FDA) and European Medicines Agency (EMA), for metastatic triple-negative breast cancer patients. In our work, we briefly summarize the various characteristics of cancer cells overexpressing Trop-2, the pre-clinical activities of specific inhibitors, and the role of anti-Trop-2 therapy in current clinical practice. We also review the ongoing clinical trials to provide a snapshot of the future developments of these therapies.
Frequency and Prognostic Relevance of Volumetric MRI Changes in Contrast- and Non-Contrast-Enhancing Tumor Compartments between Surgery and Radiotherapy of IDHwt Glioblastoma
Authors: Teske Nico , Teske Nina C. , Niyazi Maximilian , Belka Claus , Thon Niklas , Tonn Joerg-Christian , Forbrig Robert , Karschnia Philipp
Published: March 2023

In newly diagnosed IDH-wildtype glioblastoma, the frequency and prognostic relevance of tumor regrowth between resection and the initiation of adjuvant radiochemotherapy are unclear. In this retrospective single-center study we included 64 consecutive cases, for whom magnetic resonance imaging (MRI) was available for both the volumetric assessment of the extent of resection immediately after surgery as well as the volumetric target delineation before the initiation of adjuvant radiochemotherapy (time interval: 15.5 ± 1.9 days). Overall, a median new contrast-enhancement volume was seen in 21/64 individuals (33%, 1.5 ± 1.5 cm3), and new non-contrast lesion volume in 18/64 patients (28%, 5.0 ± 2.3 cm3). A multidisciplinary in-depth review revealed that new contrast-enhancement was either due to (I) the progression of contrast-enhancing tumor remnants in 6/21 patients or (II) distant contrast-enhancing foci or breakdown of the blood–brain barrier in previously non-contrast-enhancing tumor remnants in 5/21 patients, whereas it was unspecific or due to ischemia in 10/21 patients. For non-contrast-enhancing lesions, three of eighteen had progression of non-contrast-enhancing tumor remnants and fifteen of eighteen had unspecific changes or changes due to ischemia. There was no significant association between findings consistent with tumor regrowth and a less favorable outcome (overall survival: 14 vs. 19 months; p = 0.423). These findings support the rationale that analysis of the postsurgical remaining tumor-volume for prognostic stratification should be carried out on immediate postoperative MRI (<72 h), as unspecific changes are common. However, tumor regrowth including distant foci may occur in a subset of IDH-wildtype glioblastoma patients diagnosed per WHO 2021 classification. Thus, MRI imaging prior to radiotherapy should be obtained to adjust radiotherapy planning accordingly.
Social Support in a Cancer Patient-Informal Caregiver Dyad: A Scoping Review
Authors: Pasek Małgorzata , Goździalska Anna , Jochymek Małgorzata , Caruso Rosario
Published: March 2023

Social support that includes promoting healthy behaviours throughout the oncology pathway, from diagnosis to treatment to survival, can leverage existing support networks and improve the health of patients and family members in supportive roles. This scoping review aimed to identify and summarise the impact of social support on the patient-informal caregiver relationship during cancer treatment. Inclusion criteria were related to a high focus on dyadic cancer patient-informal caregiver relationships, considering a population of adult cancer patients in active hospitalisation on an oncology ward, and published between 2012 and 2022 to get a portrait of the literature that might influence the current practice. A systematic search using the “Population, Concept, and Context” framework was performed in PubMed, Web of Science, SCOPUS, EBSCO Medline, and CINAHL: 13 articles from the 16,425 pre-qualified articles published between 2012 and 2022. The narrative synthesis of the included studies highlighted that social support, encompassing its different forms within the context of dyads, is frequently associated with an enhanced quality of life, hope, and resilience of both patients and informal caregivers. However, it is important to recognize that the support interventions provided to patients, particularly caregivers, were frequently not thoroughly evaluated or explained, and the sample sizes of the included studies were often limited. Therefore, this review clarified the social and clinical potential of social support for the patient-informal caregiver relationship, paving the way for future robust studies that require to be powered and designed on specific outcomes to allow informing the practice on specific recommendations.
Dichotomous Nitric Oxide–Dependent Post-Translational Modifications of STAT1 Are Associated with Ipilimumab Benefits in Melanoma
Authors: Garg Saurabh K. , Sun James , Kim Youngchul , Whiting Junmin , Sarnaik Amod , Conejo-Garcia José R. , Phelps Mitch , Weber Jeffrey S. , Mulé James J. , Markowitz Joseph
Published: March 2023

Although Ipilimumab (anti-CTLA-4) is FDA-approved for stage III/IV melanoma adjuvant treatment, it is not used clinically in first-line therapy, given the superior relapse-free survival (RFS)/toxicity benefits of anti-PD-1 therapy. However, it is important to understand anti-CTLA-4’s mechanistic contribution to combination anti-PD-1/CTLA-4 therapy and investigate anti-CTLA-4 therapy for BRAF-wild type melanoma cases reresected after previous adjuvant anti-PD-1 therapy. Our group published that nitric oxide (NO) increased within the immune effector cells among patients with longer RFS after adjuvant ipilimumab, whereas NO increased within the immune suppressor cells among patients with shorter RFS. Herein, we measured the post-translational modifications of STAT1 (nitration-nSTAT1 and phosphorylation-pSTAT1) that are important for regulating its activity via flow cytometry and mass spectrometry approaches. PBMCs were analyzed from 35 patients undergoing adjuvant ipilimumab treatment. Shorter RFS was associated with higher pSTAT1 levels before (p = 0.007) and after (p = 0.036) ipilimumab. Ipilimumab-treated patients with high nSTAT1 levels before and after therapy in PBMCs experienced decreased RFS, but the change in nSTAT1 levels before and after ipilimumab therapy was associated with longer RFS (p = 0.01). The measurement of post-translational modifications in STAT1 may distinguish patients with prolonged RFS from ipilimumab and provide mechanistic insight into responses to ipilimumab combination regimens.
MUG CCArly: A Novel Autologous 3D Cholangiocarcinoma Model Presents an Increased Angiogenic Potential
Authors: Schrom Silke , Kleinegger Florian , Anders Ines , Hebesberger Thomas , Karner Christina , Liesinger Laura , Birner-Gruenberger Ruth , Renner Wilfried , Pichler Martin , Grillari Regina , Aigelsreiter Ariane , Rinner Beate
Published: March 2023

Cholangiocarcinoma (CCA) are characterized by their desmoplastic and hypervascularized tumor microenvironment (TME), which is mainly composed of tumor cells and cancer-associated fibroblasts (CAFs). CAFs play a pivotal role in general and CCA tumor progression, angiogenesis, metastasis, and the development of treatment resistance. To our knowledge, no continuous human in vivo-like co-culture model is available for research. Therefore, we aimed to establish a new model system (called MUG CCArly) that mimics the desmoplastic microenvironment typically seen in CCA. Proteomic data comparing the new CCA tumor cell line with our co-culture tumor model (CCTM) indicated a higher gene expression correlation of the CCTM with physiological CCA characteristics. A pro-angiogenic TME that is typically observed in CCA could also be better simulated in the CCTM group. Further analysis of secreted proteins revealed CAFs to be the main source of these angiogenic factors. Our CCTM MUG CCArly represents a new, reproducible, and easy-to-handle 3D CCA model for preclinical studies focusing on CCA-stromal crosstalk, tumor angiogenesis, and invasion, as well as the immunosuppressive microenvironment and the involvement of CAFs in the way that drug resistance develops.
Association of Clinical Aspects and Genetic Variants with the Severity of Cisplatin-Induced Ototoxicity in Head and Neck Squamous Cell Carcinoma: A Prospective Cohort Study
Authors: Macedo Ligia Traldi , Costa Ericka Francislaine Dias , Carvalho Bruna Fernandes , Lourenço Gustavo Jacob , Calonga Luciane , Castilho Arthur Menino , Chone Carlos Takahiro , Lima Carmen Silvia Passos
Published: March 2023

Background: Cisplatin (CDDP) is a major ototoxic chemotherapy agent for head and neck squamous cell carcinoma (HNSCC) treatment. Clinicopathological features and genotypes encode different stages of CDDP metabolism, as their coexistence may influence the prevalence and severity of hearing loss. Methods: HNSCC patients under CDDP chemoradiation were prospectively provided with baseline and post-treatment audiometry. Clinicopathological features and genetic variants encoding glutathione S-transferases (GSTT1, GSTM1, GSTP1), nucleotide excision repair (XPC, XPD, XPF, ERCC1), mismatch repair (MLH1, MSH2, MSH3, EXO1), and apoptosis (P53, CASP8, CASP9, CASP3, FAS, FASL)-related proteins were analyzed regarding ototoxicity. Results: Eighty-nine patients were included, with a cumulative CDDP dose of 260 mg/m2. Moderate/severe ototoxicity occurred in 26 (29%) patients, particularly related to hearing loss at frequencies over 3000 Hertz. Race, body-mass index, and cumulative CDDP were independent risk factors. Patients with specific isolated and combined genotypes of GSTM1, GSTP1 c.313A>G, XPC c.2815A>C, XPD c.934G>A, EXO1 c.1762G>A, MSH3 c.3133A>G, FASL c.-844A>T, and P53 c.215G>C SNVs had up to 32.22 higher odds of presenting moderate/severe ototoxicity. Conclusions: Our data present, for the first time, the association of combined inherited nucleotide variants involved in CDDP efflux, DNA repair, and apoptosis with ototoxicity, which could be potential predictors in future clinical and genomic models.
A Review of Literature on the Usage of Low-Cost Sensors to Measure Particulate Matter
Authors: Raysoni Amit U. , Pinakana Sai Deepak , Mendez Esmeralda , Wladyka Dawid , Sepielak Katarzyna , Temby Owen
Published: March 2023

With advances in technological sciences, individuals can utilize low-cost air monitoring sensors to record air quality at homes, schools, and businesses. Air quality data collected from LCSs are publicly accessible, informing the community of the air quality around them. It is important to measure local and regional particulate matter (PM) concentrations to keep the public involved, especially those with specific health concerns, such as asthma, wheezing, and seasonal allergies. The number of studies involving the use of LCSs to evaluate PM levels is increasing with more manufacturers producing ‘easy to use’ LCSs targeting the public. The goal of this review is to understand and incorporate the findings from studies using LCSs to analyze PM of various sizes, i.e., PM1, PM2.5, PM4, and PM10. This review integrates analyses from 51 different studies in 14 countries, including the U.S. The findings indicate spatial heterogeneity in the PM concentrations across a region. Some of the low-cost sensor manufacturers mentioned in these studies include Plantower, AQMesh, Alpha-sense, PurpleAir, E-MOTEs, and Shinyei. This review emphasizes the importance of LCSs in the field of PM monitoring and its potential to inform the public about their exposure burden, and to aid state and federal decision makers in formulating policies for mitigating the effects of PM pollution in any urban or rural setting.
Sociodemographic Variables and Body Mass Index Associated with the Risk of Eating Disorders in Spanish University Students
Authors: Escolar-Llamazares María-Camino , Martínez-Martín María-Ángeles , Medina-Gómez María-Begoña , González-Alonso María-Yolanda , Mercado-Val Elvira , Lara-Ortega Fernando
Published: March 2023

Background: The passage through university is a complex experience that can heighten personal susceptibility to eating disorders. The objective of this research is to determine how gender, age, course, educational faculty, and body mass index (BMI) can influence the risk of eating disorders among university students. Method: A transversal and descriptive study is conducted with a sample of 516 Spanish students (57.2% female, 42.8% male; Mage = 21.7, SDage = 4.1) following 26 university degrees. The Inventory Eating Disorder-Reference criterion (EDI-3-RF) was administered to the students. Contingency tables were used between categorical variables with the chi-squared statistic, at a significance level of p < 0.05. The Student t-test was used for two independent samples and a one-way ANOVA test with the post hoc Bonferroni test for more than two groups. Pearson’s correlation and a simple linear regression analysis were used to analyze the relationship between the variables in its quantitative version. Results: It was found that the female students enrolled in the second year presented a greater obsession with thinness and body dissatisfaction (p = 0.029; d = 0.338); the male students practiced more physical exercise to control their weight (p = 0.003); and that students under the age of twenty (p < 0.010; d = 0.584) and students from both the Health (p = 0.0.13) and Law (p = 0.021) educational faculties showed greater bulimic behavior (d = 0.070). More females are underweight (z = 2.8), and more men are overweight (z = 2.4). Normal-weight students scored significantly higher in thinness obsession (p = 0.033). Overweight students scored significantly higher on thinness obsession (p < 0.001) and body dissatisfaction (p < 0.001). Obese students scored significantly higher on body dissatisfaction (p = 0.04). Conclusions: The data obtained in this study, reinforce the hypothesis that the female gender, at an age within the limits of early adolescence, in the first year of the degree courses, with specific university qualifications, and a high BMI constituted factors that could provoke an eating disorder. Consequently, it is necessary to implement preventive measures adapted to the circumstances of each university student.
Comparative Transcriptome and Metabolome Analysis of Rubber Trees (Hevea brasiliensis Muell. Arg.) Response to Aluminum Stress
Authors: Ma Xiaowei , Cheng Linlin , Peng Wentao , Xie Guishui , Liu Zifan , Yang Zongming , Wang Ying , An Feng
Published: March 2023

Aluminum (Al) toxicity severely restricts crop growth and productivity in acidic soils. The rubber tree is one of the most economically important crops in tropical regions, which is tolerant to high concentrations of Al in sand or hydroponic culture conditions compared with other plants that have been reported. However, the mechanisms of Al tolerance in rubber trees remain unknown. In this study, we conducted a transcriptome and metabolome analysis for rubber tree sapling roots treated with 200 mM Al for 0 (CK), 2 or 5 days, respectively. Compared with the CK, a total of 9534 differentially expressed genes (DEGs) and 3821 differentially expressed metabolites (DEMs) were identified in 2 d of Al treatment. There were 10,373 DEGs and 4636 DEMs after 5 d of Al treatment, and 1626 DEGs and 1674 DEMs between 2 and 5 d of Al treatment. The DEGs mainly concentrated in transporters, transcription factors (TFs), cell wall biosynthesis and antioxidant systems, and the DEMs were mainly focused on lipids and lipid-like molecules, organic acids and derivatives, organic oxygen compounds, phenylpropanoids and polyketides. The combined transcriptome and metabolome analysis indicated DEGs and DEMs involved in ABC transporters, glutathione metabolism, flavonoid biosynthesis and phenylalanine metabolic pathways were identified to be closely associated with the Al tolerance of rubber trees. Our study elucidated the mechanism of rubber trees’ tolerance to Al at the transcriptional and metabolic levels, which provides a theoretical basis for the study of Al tolerance both for rubber trees and other woody plants.
Shoot Phenology as a Driver or Modulator of Stem Diameter Growth and Wood Properties, with Special Reference to Pinus radiata
Authors: Burdon Rowland D.
Published: March 2023

Seasonal phenology is expressed in the annual rhythms of growth and quiescence, which may range from being sharply defined to weakly quantitative. These rhythms, both vegetative and reproductive, are a key aspect of a plant’s survival strategy. They enable the plant to both survive seasonal stresses and take advantage of favourable conditions, while making pollination efficient through synchronised flowering. Maturation in woody perennials causes some ontogenetic modulation of seasonal phenology. Shoot phenology is driven by various environmental cues, notably temperatures and daylength, with shoot extremities often being prime receptors. The phenology of shoot extremities is in turn seen as a strong driver of cambial activity, which itself is an aspect of shoot phenology and the basis of stem diameter growth. The aspects of cambial activity reflecting hoot–tip phenology primarily involve the timing of xylem formation and the anatomical and physical properties of xylem cells. The actual amount of diameter growth, however, is governed much more by other factors, notably the growth potential of the species, the space for the tree to grow in, and how favourable local conditions are for growth. Somehow, all tree species allocate resources to shoot extension and diameter growth to represent viable adaptive strategies, although there is no identified role of phenology per se in such allocation. Among species, Pinus radiata is extremely important commercially, largely through a high growth potential that reflects distinctive phenology. Much research on the species provides a basis for linking its phenology and shoot–tip architecture to seasonal timing of diameter growth and fine details of within-ring variation in xylem cell properties. This provides a baseline for comparison with many other tree species, conifers and hardwoods. Selected examples are considered to illustrate the range of adaptive strategies. Regulation of diameter growth and internal variation in wood properties reflects the response to competitive pressures. Modifying the regulation to optimise wood production and quality poses a great challenge for future domestication.
DataStream XES Extension: Embedding IoT Sensor Data into Extensible Event Stream Logs
Authors: Mangler Juergen , Grüger Joscha , Malburg Lukas , Ehrendorfer Matthias , Bertrand Yannis , Benzin Janik-Vasily , Rinderle-Ma Stefanie , Serral Asensio Estefania , Bergmann Ralph
Published: March 2023

The Internet of Things (IoT) has been shown to be very valuable for Business Process Management (BPM), for example, to better track and control process executions. While IoT actuators can automatically trigger actions, IoT sensors can monitor the changes in the environment and the humans involved in the processes. These sensors produce large amounts of discrete and continuous data streams, which hold the key to understanding the quality of the executed processes. However, to enable this understanding, it is needed to have a joint representation of the data generated by the process engine executing the process, and the data generated by the IoT sensors. In this paper, we present an extension of the event log standard format XES called DataStream. DataStream enables the connection of IoT data to process events, preserving the full context required for data analysis, even when scenarios or hardware artifacts are rapidly changing. The DataStream extension is designed based on a set of goals and evaluated by creating two datasets for real-world scenarios from the transportation/logistics and manufacturing domains.
Approximate Solutions for Time-Fractional Fornberg–Whitham Equation with Variable Coefficients
Authors: Alsidrani Fahad , Kılıçman Adem , Senu Norazak
Published: March 2023

In this research, three numerical methods, namely the variational iteration method, the Adomian decomposition method, and the homotopy analysis method are considered to achieve an approximate solution for a third-order time-fractional partial differential Equation (TFPDE). The equation is obtained from the classical (FW) equation by replacing the integer-order time derivative with the Caputo fractional derivative of order η=(0,1] with variable coefficients. We consider homogeneous boundary conditions to find the approximate solutions for the bounded space variable l<χ<L and l,L∈R. To confirm the effectiveness of the proposed methods of non-integer order η, the computation of two test problems was presented. A comparison is made between the obtained results of the (VIM), (ADM), and (HAM) through tables and graphs. The numerical results demonstrate the effectiveness of the three numerical methods.
How Physical and Molecular Anthropology Interplay in the Creation of Biological Profiles of Unidentified Migrants
Authors: Pilli Elena , Palamenghi Andrea , Morelli Stefania , Mazzarelli Debora , De Angelis Danilo , Jantz Richard L. , Cattaneo Cristina
Published: March 2023

The skeletal sex and ancestry of unidentified human crania can be inferred both from physical and from molecular features. This paper depicts and discusses the experiences of physical and molecular anthropologists on a set of commingled crania from the largest Mediterranean shipwreck disaster on 18 April 2015, in order to facilitate identification of human crania. Twenty-one disarticulated crania that were recovered from the above-mentioned shipwreck were analyzed to estimate skeletal sex and ancestry, following a physical and a molecular pipeline. The physical analyses applied morphological and metric methods that provided posterior probabilities for the crania to be classified into a sex or ancestral group. The molecular analyses were performed on petrous bones via a shotgun sequencing approach that allowed us to determine the sex of each individual and to retrieve the complete mitochondrial genome, Y chromosome single nucleotide polymorphisms, up to 597573 SNPs across the human genome from each individual. The morphometric sex analyses showed that most crania belonged to male individuals, although some estimations remained uncertain or undetermined. Inconsistent results were obtained for ancestry estimation as well, since morphological methods classified the crania mostly as European/White, in contrast to the most numerous African forms determined by craniometric analyses. This quite agreed with molecular analyses that identified only African males. Overall, undetermined and contrasting results were obtained between disciplines, preventing the creation of reliable and sound biological profiles that could provide guidance on the sex and ancestral group of the victims. Therefore, the times may not be mature for a merger of physical and molecular anthropology. However, future investigations of this research avenue would pave the way to the possible development of novel tools, methods, and wider reference databases that could address the limitations of both disciplines.
High Performance of a Dominant/X-Linked Gene Panel in Patients with Neurodevelopmental Disorders
Authors: Spataro Nino , Trujillo-Quintero Juan Pablo , Manso Carmen , Gabau Elisabeth , Capdevila Nuria , Martinez-Glez Victor , Berenguer-Llergo Antoni , Reyes Sara , Brunet Anna , Baena Neus , Guitart Miriam , Ruiz Anna
Published: March 2023

Neurodevelopmental disorders (NDDs) affect 2–5% of the population and approximately 50% of cases are due to genetic factors. Since de novo pathogenic variants account for the majority of cases, a gene panel including 460 dominant and X-linked genes was designed and applied to 398 patients affected by intellectual disability (ID)/global developmental delay (GDD) and/or autism (ASD). Pathogenic variants were identified in 83 different genes showing the high genetic heterogeneity of NDDs. A molecular diagnosis was established in 28.6% of patients after high-depth sequencing and stringent variant filtering. Compared to other available gene panel solutions for NDD molecular diagnosis, our panel has a higher diagnostic yield for both ID/GDD and ASD. As reported previously, a significantly higher diagnostic yield was observed: (i) in patients affected by ID/GDD compared to those affected only by ASD, and (ii) in females despite the higher proportion of males among our patients. No differences in diagnostic rates were found between patients affected by different levels of ID severity. Interestingly, patients harboring pathogenic variants presented different phenotypic features, suggesting that deep phenotypic profiling may help in predicting the presence of a pathogenic variant. Despite the high performance of our panel, whole exome-sequencing (WES) approaches may represent a more robust solution. For this reason, we propose the list of genes included in our customized gene panel and the variant filtering procedure presented here as a first-tier approach for the molecular diagnosis of NDDs in WES studies.
Scanning Electron and Atomic Force Microscopic Analysis of Erythrocytes in a Cohort of Atopic Asthma Patients—A Pilot Study
Authors: Alummoottil Sajee , van Rooy Mia , Bester Janette , Grobbelaar Craig , Phulukdaree Alisa
Published: March 2023

Background: Non-communicable diseases are often associated with chronic inflammation, placing patients suffering from these conditions at a higher risk of thrombosis and other complications. The pathophysiology of asthma and/or atopic asthma is also linked to chronic inflammation, which consequently may alter blood parameters including erythrocyte structure and function. Methodology: The objective of this study was to evaluate differences in erythrocytes between patients with atopic asthma (n = 30) and healthy individuals (n = 30) by evaluating routine haematological parameters; structures and axial ratios of erythrocytes using light microscopy; erythrocyte membrane elasticity using atomic force microscopy; and erythrocyte ultrastructure using scanning electron microscopy. Results: The haematological findings of healthy participants and patients suffering from asthma were within normal clinical ranges together with significantly higher levels of circulating monocytes (p = 0.0066), erythrocytes (p = 0.0004), haemoglobin (p = 0.0057), and haematocrit (p = 0.0049) in asthma patients. The analysis of eosin-stained erythrocytes by light microscopy showed more echinocytes, acanthocytes, and ovalocytes compared to controls and a significant difference in axial ratios (p < 0.0001). Atomic force microscopy findings showed reduced erythrocyte membrane elasticity in asthmatic erythrocytes (p = 0.001). Ultrastructural differences in erythrocytes were visible in the asthma group compared to controls. Conclusion: Altered erythrocyte ultrastructural morphology and a significant change in the haematological profile are evident in atopic asthma and may influence common complications associated with asthma. The impact of these changes on the physiological mechanisms of coagulation and the pathophysiology of asthma needs to be further elucidated.
Extramedullary T-lymphoblastic Crisis in a Myelodysplastic/Myeloproliferative Neoplasm with a t(12;22)/MN1::ETV6 Translocation
Authors: Freitas Ana Carolina , Maia Tiago , Desterro Joana , Pierdomenico Francesca , Nunes Albertina , Ferreira Isabelina , Cabeçadas José , Gomes da Silva Maria
Published: March 2023

Myelodysplastic/myeloproliferative neoplasms (MDS/MPN) are not a single disease, but rather a heterogenous group of entities which are increasingly subclassified according to recurrent genetic abnormalities. Chromosomal translocations involving meningioma 1 (MN1) and ETS variant 6 (ETV6) genes are extremely rare, but recurrent in myeloid neoplasms. We describe the case of a patient with a myelodysplastic/myeloproliferative neoplasm with neutrophilia, who developed an extramedullary T-lymphoblastic crisis with the t(12;22)(p13;q12) translocation as the only cytogenetic abnormality. This case shares several clinical and molecular features with myeloid/lymphoid neoplasms with eosinophilia. The treatment of this patient was challenging, as the disease proved to be highly refractory to chemotherapy, with allogenic stem cell transplantation as the only curative option. This clinical presentation has not been reported in association with these genetic alterations and supports the concept of a hematopoietic neoplasm originating in an early uncommitted precursor cell. Additionally, it stresses the importance of molecular characterization in the classification and prognostic stratification of these entities.
The Application of Entomophagous and Acariphagous Species in Biological Protection Systems of an Apple Orchard (Malus domestica Borkh)
Authors: Ismailov Vladimir , Agasyeva Irina , Nastasy Anton , Nefedova Maria , Besedina Ekaterina , Komantsev Alexandr
Published: March 2023

The systematic and long-term use of pesticides in fruit plantations leads to the formation of resistant pest populations. The purpose of this work was to evaluate the effectiveness of the use of entomophages and acariphages for the protection of apple orchards. Against the dominant pest Cydia pomonella (Linnaeus), Habrobracon hebetor (Say) was used, which was caught in the Krasnodar Territory using cassettes with caterpillars attractive to H. hebetor. To determine the most genetically high-quality population, an RAPD analysis was carried out from three Russian (Krasnodar, Stavropol, and Belgorod) and one Kazakh (Shymkent) populations of H. hebetor, which revealed a high level of DNA polymorphism and genetic diversity in the studied geographical populations of the cities of Krasnodar and Stavropol. The efficiency of the captured Krasnodar population of H. hebetor against C. pomonella was about 75%. To regulate the number of aphids Aphis pomi De Geer and Tetraneura caerulescens (Pass.), breeding reserves of the aphidophages Harmonia axyridis Pallas, Leis dimidiata Fabr., Cycloneda sangvinea L., and Aphidius colemani Vier. were established. The biological efficiency of the developed technique was 82.8–88.6%. The release of the acariphages Amblyseius andersoni (Chant) and Metaseiulus occidentalis (Nesb.) on the apple tree showed effectiveness from 80 to 90% against Tetranychus urticae Koch and Panonychus ulmi (Koch). To study the possibility of simultaneous use of entomophages and insecticides, experiments were carried out to study the sensitivity of H. hebetor and H. axiridis to insecticides. When H. hebetor cocoons were treated with Insegar® and Atabron®, the ectoparasitoid emergence values were 98.4% and 100%, respectively. The survival of adult H. axiridis treated with Madex twin®, Atabron®, and Koragen® on the fifth day was 97.3%, 89.6%, and 81.9%, respectively. Based on the data obtained, it can be argued that it is possible to create favorable conditions for entomophages, which effectively regulate pest numbers in apple orchards.
How Did People with Prediabetes Who Attended the Diabetes Prevention Education Program (DiPEP) Experience Making Lifestyle Changes? A Qualitative Study in Nepal
Authors: Shakya Pushpanjali , Bajracharya Monish , Skovlund Eva , Shrestha Abha , Karmacharya Biraj Man , Kulseng Bård Eirik , Sen Abhijit , Steinsbekk Aslak , Shrestha Archana
Published: March 2023

Diabetes can be prevented through lifestyle modification in the prediabetic phase. A group-based lifestyle intervention called ‘Diabetes Prevention Education Program’ (DiPEP) was tested recently in Nepal. The present study aimed to explore experiences of making lifestyle changes among people with prediabetes participating in the DiPEP. This qualitative study, with semi-structured interviews of 20 participants, was conducted 4–7 months following DiPEP intervention. Data analysis was performed by thematic analysis. The results included four themes, understanding that diabetes could be prevented, lifestyle changes made, hurdles to overcome, and experiencing benefits leading to sustained change. Some participants said they felt relieved to know that they had a chance to prevent diabetes. The participants talked mostly about making changes in diet (reducing carbohydrate intake) and physical activity (starting exercises). Obstacles mentioned included a lack of motivation and a lack of family support to implement changes. Experiencing benefits such as weight loss and reduced blood sugar levels were reported to lead them to maintain the changes they had made. Understanding that diabetes could be prevented was a key motivator for implementing changes. The benefits and hurdles experienced by the participants of the present study can be taken into consideration while designing lifestyle intervention programs in similar settings.
Special Issue: Place, Space, and Mental Health
Authors: Pasanen Tytti P. , Kajosaari Anna
Published: March 2023

Global megatrends, including climate change and urbanisation, are shaping and changing how we live in the future [...]
Pleurotus pulmonarius Strain: Arsenic(III)/Cadmium(II) Accumulation, Tolerance, and Simulation Application in Environmental Remediation
Authors: Zhang Yuhui , Chen Xiaohong , Xie Ling
Published: March 2023

The arsenic (As, III) and cadmium (Cd, II) accumulation and tolerance traits of a new strain Pleurotus pulmonarius MT were evaluated, and the utilization of the strain for repairing contaminated liquid and soil was explored. The hypha cultivated in potato dextrose agar (PDA) exhibited medium or high Cd accumulation (0 to 320 mg/L), medium Cd tolerance (maximum tolerated concentration, MTC ≥ 640 mg/L), medium As accumulation (0 to 80 mg/L), and high As tolerance (MTC > 1280 mg/L). The hypha has application potential in processes related to the removal of Cd and As in aqueous pollutants at concentrations of 80 mg/L Cd and 20 mg/L As. The trends obtained for the fruiting bodies of P. pulmonarius MT seemed to deviate from those of the hypha of this strain. The results show that the fruiting bodies featured medium As accumulation (0 to 40 mg/kg), medium As tolerance (MTC > 160 mg/kg), medium Cd accumulation (0 to 10 mg/kg), and high Cd tolerance (MTC > 1280 mg/kg). The fruiting bodies of P. pulmonarius MT were utilized in processes related to the recovery of Cd and As in substrates, that is, 12% contaminated soil mixed with 50 mg/kg Cd and 200 mg/kg As; thus, the hypha and fruiting bodies of P. pulmonarius MT can be used for the decontamination of water and soil containing As(III) and Cd(II).
Cognition and Mental Health in Pediatric Patients Following COVID-19
Authors: Avittan Hadar , Kustovs Dmitrijs
Published: March 2023

The global coronavirus pandemic has significantly impacted public health and has been a research subject since its emergence in 2019. The acute phase of the disease leads to pulmonary and non-pulmonary manifestations, which in some individuals may progress to long-lasting symptoms. In this article, we conducted a narrative review of the current literature to summarize current knowledge regarding long COVID syndrome in children, focusing on cognitive symptoms. The review included a search of three databases (PubMed, Embase, and Web of Science) using the key phrases “post COVID-19 cognitive pediatric”, “long COVID pediatric”, “mental health long COVID children”, and “COVID-19 cognitive symptoms”. A total of 102 studies were included. The review revealed that the main long-term cognitive symptoms following COVID-19 were memory and concentration deficits, sleep disturbances, and psychiatric states such as anxiety and stress. In addition to the direct physiological effects of a viral infection, there are psychological, behavioral, and social factors contributing to cognitive impairment, which should be addressed regarding the pediatric population. The high prevalence of neurocognitive symptoms in children following COVID-19 emphasizes the importance of understanding the mechanisms of nervous system involvement.