bluuebunny/crossref_metadata_2025 huggingface dataset:
Towards a blue-sky target in the EU and Turkey: exploration on the connection between CO2 emissions and the air transportation Sector
Authors: Pac Dogukan Ayberk , Oner Yıldırım
Published: August 2023

Air transportation sector over the years has experience tremendous growth, being recognized as an integral part of the global transport system. However, the growth in the transportation system is not void of environmental concerns. The coronavirus disease had a devastating impact on the airline industry worldwide, with almost all air travel suspended in late March till April 2021. Since then, cancellation of flights has enormously reduced CO2 emission in the air caused by air transportation. Negative impact on economy due to contraction in airline industry turn out to be positive impact on an environmental sustainability. Airline sector has been the first and most obvious economic casualty of the coronavirus. International passenger air traffic was crashed with 98 percent of cancellation in air within both domestic and international flying routes due to lockdowns in all airports. To this end, the present study empirically explores the environmental impact of the sectoral lockdown within the EU28 and Turkey by leveraging the Wavelet Coherence Transform (WCT) technique. The result revealed that there was a sharp decline in CO2 emissions caused by the airline industry during the period of 2020–2021 on quarterly basis. The recovery of international passenger traffic will proceed at a very slow pace. From a policy perspective, stakeholders in the airline sector are encouraged to formulate strategies that promote a balance between economic growth without compromising environmental quality within the contact of the investigated bloc.
Diagnostic accuracy of digital solutions to screen for cognitive impairment: a systematic review and meta-analysis
Authors: Magno Marisa , Martins Ana Isabel , Pais Joana , Silva Anabela G. , Rocha Nelson Pacheco
Published: August 2023

Early detection of cognitive impairment is essential to initiate intervention and guarantee access to healthcare services. Digital solutions are emerging in literature as an alternative approach to cognitive screening. Our primary goal is to synthesize the evidence on digital solutions' diagnostic ability/accuracy to screen for cognitive impairment. A secondary goal is to distinguish whether the ability to screen cognitive impairment varies as a function of the type of digital solution: paper-based or innovative digital solutions. A systematic review and meta-analysis of digital solutions' diagnostic accuracy were conducted, including 25 studies. Digital solutions presented a variable diagnostic accuracy range. Innovative digital solutions offered at least 0.78 of sensitivity but showed lower specificity levels than the other subgroup. Paper-based digital solutions revealed at least 0.72 of specificity, but sensitivity started at 0.49. Most digital solutions do not demand the presence of a trained professional and include an automatic digital screening system and scoring, which can enhance cognitive screening and monitoring. Digital solutions are adequate for cognitive screening in the community and clinical practice showing sensitivity and specificity levels similar to traditional widely used paper and pencil tests. A detailed assessment of the accuracy levels of each digital solution is recommended.
The indigenous bioculture of the Pungalá Parish of Ecuador an approach to their culinary and medicinal heritage.
Authors: zurita Ronald , Bastidas-Arauz Maria Belen , Saeteros-Hernandez Angelica , Chávez Renato Hernán Herrera , Cardenas-Moyano Maria
Published: August 2023

The food and culinary heritage with medicinal uses is a fascinating field that combines history, culture, and health through food. Over the centuries, various cultures have developed knowledge and practices related to the use of specific ingredients and culinary preparations with medicinal properties. In the Pungalá parish in Ecuador, these generational knowledge and traditions form a valuable biocultural heritage that helps us understand the close relationship between food and traditional medicine. Through qualitative and documentary research, interviews and community workshops are conducted to gather information using different observation techniques. Among the findings regarding the food and culinary heritage of Pungalá are extinct culinary traditions, as well as important culinary techniques and processes that play a role in nutrition and medicine, valuing traditional and ancestral knowledge as part of their culture. The inventory includes 21 plants used in Andean medicine and 38 preparations, including beverages, porridges, stews, soups, and wrapped dishes. It is concluded that this knowledge constitutes a valuable food and culinary heritage, and its biocultural is based on ancestral knowledge of the healing properties of local plants and foods, contributing to the health and identity of the community. Measures should be taken to preserve and promote this heritage in the face of current challenges.
Measuring Stigma Cultures in Healthcare Settings: Development and Testing of a New Measure
Authors: Stuart Heather , Knaak Stephanie
Published: August 2023

Background
Organizational cultures represent the norms, values, and assumptions underlying day to data activities and interactions. People with a mental or substance use disorder experience considerable stigma when interacting with healthcare cultures such as feeling patronized, humiliated, and excluded from treatment decisions. The management of healthcare cultures, and specifically stigma cultures, is increasingly viewed as a key aspect of healthcare reform. To evaluate the effectiveness of interventions to reduce stigma cultures in healthcare, valid and reliable measures are needed. Numerous measures assess aspects of the quality of care provided to people with a mental or substance use disorder, but none specifically addresses stigma cultures. To fill this gap, we developed and tested a new mental illness related stigma measure to be used in healthcare organizations to assess stigma cultures of care.
Methods
Item generation was grounded in the lived experiences of people (n = 20) with a mental or substance use disorder and reflect their personal experiences with healthcare encounters for physical or emotional health care. Items are rated on a 4-point agreement scale with higher scores indicating higher stigma. Following the qualitative analysis, national survey data (n = 2,476) were collected to conduct an exploratory and confirmatory factor analysis on split halves of the sample.
Results
The analyses provided statistical support for a 23-item unidimensional scale that could be used in any healthcare setting to assess the presence of a stigma culture. Reliability was high (.92) and the resulting scale scores (ranging from 0 to 92) were approximately normal.
Conclusions
The resulting Stigma Cultures in Healthcare scale can be used across a range of physical and mental healthcare settings to assess the extent to which the culture of care is experienced as stigmatizing by clients with mental or substance use disorders.
Analysis of Risk Factors for Leukoaraiosis: A Multicenter Retrospective Study
Authors: Sun Lingqi , Li Yi , Chen Xian , Guo Wang , Fang Li , Ma Ji
Published: August 2023

Background: Leukoaraiosis is a common imaging marker of cerebral small vessel disease. There is now increasing evidence that it is associated with cognitive impairment and a high risk of death after stroke, among others. The aim of this study was to analyze and screen the risk factors clinically associated with the development of leukoaraiosis, and to explore clinical biomarkers that may predict leukoaraiosis.
Methods: Inpatients were continuously recruited from July 2014 to October 2020. After admission, the cranial MRI examination was evaluated, and the severity of leukoaraiosis were evaluated and graded. Vascular risk factors and relevant clinical data were collected. Univariate analysis was used to analyze the parameters, and multivariate logistic regression analysis was used to analyze the statistically significant parameters. The analysis results were plotted as ROC curve to find out the diagnostic accuracy of the model.
Results: 1), 327 patients meeting the study criteria were included. Univariate analysis showed that 13 factors were statistically significantly (P < 0.05). 2), Multivariate logistic regression model showed that age, elevated systolic blood pressure, ischemic stroke and FT4 were independent risk factors for leukoaraiosis. 3), The ROC curve indicated the accuracy of diagnosis on leukoaraiosis is 0.906, and the positive rate and negative rate are both 85.2%.
Conclusions: 1), Factors affecting the development of leukoaraiosis include age, systolic blood pressure, ischemic stroke, and FT4 level. 2), The model of "age, systolic blood pressure, ischemic stroke, FT4" have relatively ideal sensitivity and specificity in predicting the development of leukoaraiosis.
First evidence for non 1− pygmy dipole resonance in neutron-rich nuclei
Authors: Li R. , Ponomarev V. Yu. , Harakeh M. N. , Ayoubi L. , Falou H. Al , Benzoni Giovanna , Blanc F. , Bozkurt V. , Ciemala Michal , Delafosse Clement , Deloncle I. , Didierjean F. , Ebata S. , Fallot M. , Gaulard C. , Gottardo Andrea , Guadilla V. , Guillot J. , Hadyńska-Kl˛ek K. , Ibrahim F. , Jovancevic Nikola , Kankainen Anu , Lebois Mathieu , Matea I. , Napiorkowski P. , Roussiere B. , Sobolev Yu. , Stefan I. , Stukalov S. , Thisse D. , Tocabens G. , Verney D.
Published: August 2023

We predict the existence of medium spin PDR and find experimental evidence in 80Ge using β-decay of 80Ga. This nucleus was produced in the photo-fission of 238U. A hybrid γ-ray spectrometer was developed using a plastic detector for β-tagging, HPGe detectors for low-energy γ rays detection and phoswich detectors of PARIS for high-energy γ rays. The low-high energy coincidence spectrum shows two peaks at 7181(53) and 7337(53) keV in the β-decay of the 80mGa isomeric state only, in coincidence with the 21+ → 0+ transition of 80Ge. Combined with decay information, electric dipole character (E1) were assigned to these γ rays which de-excite 7.84 MeV and 7.996 MeV states. Based on this fact and the comparison with theoretical calculations, we conclude that these enhanced E1 transitions are driven by neutron-skin oscillation of 80Ge. The oscillation is triggered by a conversion of a deeply-bound neutron from the Z=N=28 proton-neutron saturated core into a proton in the Fermi surface. These results provide an evidence for a PDR state with spin different from 1− and built on a low-energy excited state. Consequently, it extends the PDR’s boundary along spin dimension for the first time and provides experimental data for testing the validity of the Brink-Axel hypothesis in the PDR region.
Interventions to increase vaccination in vulnerable groups: rapid overview of reviews
Authors: Norman Gill , Kletter Maartje , Dumville Jo
Published: August 2023

Objective
Groups which are marginalised, disadvantaged or otherwise vulnerable have lower uptake of vaccinations. This differential has been amplified in COVID-19 vaccination compared to (e.g.) influenza vaccination. This overview assessed the effectiveness of interventions to increase vaccination in underserved, minority or vulnerable groups.
Methods
In November 2022 we searched four databases for systematic reviews including RCTs of interventions to increase vaccination in underserved, minority or vulnerable groups. We used rapid review methods to screen, extract data and assess risk of bias in identified reviews. We undertook narrative synthesis using an approach modified from SWiM guidance. We categorised interventions as being high, medium or low intensity, and as targeting vaccine demand, access, or providers.
Results
We included 23 systematic reviews, including studies in high and low or middle income countries, focused on children, adolescents and adults. Groups were vulnerable based on socioeconomic status, minority ethnicity, migrant/refugee status, age, location or LGBTQ identity. Pregnancy/maternity sometimes intersected with vulnerabilities. Evidence supported interventions including: home visits to communicate/educate and to vaccinate, and facilitator visits to practices (high intensity); telephone calls to communicate/educate, remind/book appointments (medium intensity); letters, postcards or text messages to communicate/educate, remind/book appointments and reminder/recall interventions for practices (low intensity). Many studies used multiple interventions or components.
Conclusion
There was considerable evidence supporting the effectiveness of communication in person, by phone or in writing to increase vaccination. Both high and low intensity interventions targeting providers showed effectiveness. Limited evidence assessed additional clinics or targeted services for increasing access; only home visits had higher confidence evidence showing effectiveness. There was no evidence for interventions for some communities, such as religious minorities which may intersect with gaps in evidence for additional services. None of the evidence related to COVID-19 vaccination where inequalities of outcome are exacerbated.
Prospero registration: CRD42021293355
The Effect of Cuff Arthropathy Stage on Sleep Disturbance and Kinesiophobia in Reverse Shoulder Arthroplasty Patients
Authors: ILYAS Gokhan , EGELI Ercument , IPCI Fikri Burak , GOKALP Oguzhan
Published: August 2023

Background
The aim of the current study was to determine the changes in pre-and postoperative Pittsburg sleep quality index (PSQI) and Tampa scale of kinesiophobia (TSK) values ​​according to the Hamada classification in patients who underwent reverse shoulder arthroplasty (RSA) for rotator cuff tear arthropathy (RCTA).
Methods
One hundred and eight patients who underwent RSA for RCTA were reviewed retrospectively. The patients were divided into two groups with low grade (stages 1-2-3) (n = 49) and high grade (stages 4a-4b-5) (n = 59) according to the Hamada classification, which is the radiographic evaluation of RCTA. PSQI and TSK values ​​were calculated preoperatively, and post-operatively at the 6th week, 6th month, and 1st year. The change in PSQI and TSK values ​​between the evaluations and the effect of staging according to the Hamada classification on this change was examined.
Results
When compared in preoperative evaluations, PSQI and TSK scores were found to be lower in low-grade group 1 (7.39 ± 1.56, 51.88 ± 4.62, respectively) than in high-grade group 2 (10.47 ± 2.39, 57.05 ± 3.25, respectively) according to Hamada classification (both p < 0.001). In the postoperative evaluations, PSQI and TSK results decreased gradually compared to the preoperative evaluations, and there was a dramatic decrease in both parameters between the 6th-week and 6th-month evaluations (both p < 0.001). Preoperatively, 102 (95%) patients had sleep disturbance (PSQI ≥ 6), and 108 (100%) patients had high kinesiophobia (TSK > 37). In the 1st year follow-ups, sleep disturbance was observed in 5 (5%) patients and kinesiophobia in 1 (1%) patient. When the Hamada stages were compared, it was seen that there was a significant difference before the operation (both p < 0.001), but the statistically significant difference disappeared in the PSQI value in the 1st year (p = 0.092) and in the TSK value in the 6th month (p = 0.164) post-operatively. It was observed that Hamada staging caused significant differences in PSQI and TSK values ​​in the preoperative period but did not affect the clinical results after treatment.
Conclusions
RSA performed based on RCTA improves sleep quality and reduces kinesiophobia. RCTA stage negatively affects PSQI and TSK before the operation but does not show any effect after the treatment.
A methodology for multiple performances optimization of computer numerical controlled (CNC) machining process based on design of experiment, multi-criteria decision-making and multiple regression model
Authors: Ryang Si-Ho , Yang Won-Chol , Kuon Chang-Hyok , Kim Chol-Sok , Kim Yong-Il
Published: August 2023

This paper proposes multiple performances optimization methodology for computer numerical controlled (CNC) machining based on Taguchi method, multi-criteria decision making (MCDM) and multiple regression (MR) model. It consists of the following steps: (1) setting levels of process parameters and selecting appropriate orthogonal array (OA), (2) arranging the parameters on OA and measuring machining performance values at every trials, (3) calculating comprehensive performance (CP) by integrating the multiple performances using a reasonable MCDM, (4) developing MR model between CP and process parameters, (5) analyzing influence of process parameters using correlation analysis and Taguchi method, and (6) determining optimal process parameters using Taguchi and grid search methods. The methodology is applied to determine optimal turning process parameters for optimizing multiple machining performances in the high speed CNC turning of AISI P20 tool steel. It may be widely applicable to solve many practical multiple performances optimization problems in manufacturing.
Impact of KRAS Mutations and Co-mutations on Clinical Outcome in Pancreatic Ductal Adenocarcinoma
Authors: Zhao Dan , Yousef Abdelrahman , Yousef Mahmoud , Chowdhury Saikat , Abdilleh Kawther , Knafl Mark , Edelkamp Paul , Alfaro-Munoz Kristin , Chacko Ray , Peterson Jennifer , Smaglo Brandon , Wolff Robert A. Wolff , Pant Shubham , Lee Michael , Willis Jason , Overman Michael , Doss Sudheer , Matrisian Lynn , Hurd Mark , Snyder Rebecca , Katz Matthew , Wang Huamin , Maitra Anirban , Shen John Paul
Published: August 2023

The relevance of KRAS mutation alleles to clinical outcome remains inconclusive in pancreatic adenocarcinoma (PDAC). We conducted a retrospective study of 803 PDAC patients (42% with metastatic disease) at MD Anderson Cancer Center. Overall survival (OS) analysis demonstrated that KRAS mutation status and subtypes were prognostic (p<0.001). Relative to patients with KRAS wildtype tumors (median OS 38 months), patients with KRASG12R had a similar OS (median 34 months), while patients with KRASQ61 and KRASG12D mutated tumors had shorter OS (median 20 months [HR: 1.9, 95% CI 1.2-3.0, p=0.006] and 22 months [HR: 1.7, 95% CI 1.3-2.3, p<0.001], respectively). There was enrichment of KRASG12D mutation in metastatic tumors (34% vs 24%, OR: 1.7, 95% CI 1.2-2.4, p=0.001) and enrichment of KRASG12R in well and moderately differentiated tumors (14% vs 9%, OR: 1.7, 95% CI 1.05-2.99, p=0.04). Similar findings were observed in the external validation cohort (PanCAN’s Know Your Tumor® dataset, n=408).
Ethnicity-specific BRCA1, BRCA2, PALB2, and ATM pathogenic alleles in breast and ovarian cancer patients from the North Caucasus
Authors: Sokolenko Anna P. , Bakaeva Elvina Kh. , Venina Aigul R. , Kuligina Ekaterina Sh. , Romanko Alexandr A. , Aleksakhina Svetlana N. , Belysheva Yana V. , Belogubova Evgeniya V. , Stepanov Ilya A. , Zaitseva Olga A. , Yatsuk Olga S. , Togo Alexandr V. , Khamgokov Zaur M. , Kadyrova Azinat O. , Pirmagomedov Albert Sh. , Bolieva Marina B. , Epkhiev Alexandr A. , Tsutsaev Aslan K. , Chakhieva Madina D. , Khabrieva Khalimat M. , Khabriev Idris M. , Murachuev Mirza A. , Buttaeva Bella N. , Baboshkina Liliya S. , Bayramkulova Fatima I. , Katchiev Islam R. , Alieva Lina Kh. , Raskin Grigory A. , Orlov Sergey V. , Khachmamuk Zarema K. , Levonyan Karine R. , Gichko Dariya M. , Kirtbaya Dmitriy V. , Degtyariov Alexey M. , Sultanova Luisa V. , Musayeva Hedi S. , Belyaev Alexey M. , Imyanitov Evgeny N.
Published: August 2023

Background
Mountain areas of the North Caucasus host several large ethnic communities that preserved their national identity over the centuries.
Methods
This study involved high-grade serous ovarian cancer (HGSOC) and breast cancer (BC) patients from Dagestan (HGSOC: 37; BC: 198), Kabardino-Balkaria (HGSOC: 68; BC: 155), North Ossetia (HGSOC: 51; BC: 104), Chechnya (HGSOC: 68; BC: 79), Ingushetia (HGSOC: 19; BC: 103), Karachay-Cherkessia (HGSOC: 13; BC: 47), and several Armenian settlements (HGSOC: 16; BC: 101). The group of BC patients was enriched by young-onset and/or family history-positive and/or bilateral and/or receptor triple-negative cases. The entire coding region of BRCA1, BRCA2, PALB2, and ATM genes was analyzed by next-generation sequencing.
Results
A significant contribution of BRCA1/2 pathogenic variants (PVs) in HGSOC and BC development was observed across all North Caucasus regions (HGSOC: 18.8–38.5%; BC: 6.3–13%). Founder alleles were identified in all ethnic groups studied, e.g., BRCA1 c.3629_3630delAG in Chechens, BRCA2 c.6341delC in North Ossetians, BRCA2 c.5351dupA in Ingush, BRCA1c.2907_2910delTAAA in Karachays, etc. Some BRCA1/2alleles, particularly BRCA2c.9895C>T, were shared by several nationalities. ATM PVs were detected in 14 patients, with c.1673delG and c.8876_8879delACTG alleles occurring twice each. PALB2 heterozygosity was observed in 5 subjects, with one variant seen in 2 unrelated women.
Conclusion
This study adds to the evidence for the global-wide contribution of BRCA1/2 genes to BC and HGSOC morbidity, although the spectrum of their PVs is a subject of ethnicity-specific variations. The data on founder BRCA1/2alleles may be considered when adjusting the BRCA1/2 testing procedure to the ethnic origin of patients.
Exploring the Impact of COVID-19 Information Acquisition on University Students' Psychological Capital: The Mediating Roles of Risk Perception and Emotional Coping in a Chain Model
Authors: Han Bingxu
Published: August 2023

Background
University students in the 2022 Shanghai epidemic went through monthslong city-wide lockdowns, during which period, social media platforms were primary ways for this population to acquire COVID-19 related information. Psychological health of young adults on campus is concerned throughout this research. This study integrated the theory of psychological capital (PsyCap) in research whether and how social media information overload could impact students’ psychological capital under the context of 2022 COVID-19 outbreak in Shanghai. Findings from this research shed light on the underpinning mechanism of whether and how social media information overload shapes risk perception in the student population within Shanghai, and explore how students’ correspondingly emotional coping associates with their psychological inner being.
Methods
A cross-sectional study among students who encountered with emergency outbreaks in Shanghai from March to June, 2022 was conducted. Over 400 valid samples were used to construct the following analysis. Hierarchical linear regression analysis was conducted using SPSS Statistics Version 26. The chain mediated model was verified using PROCESS.
Results
Information overload is positively related to personal risk perception and societal risk perception. Risk perception leads to emotional coping in university students, which correspondingly predicts the psychological capital of these individuals in face of public health emergencies. Though information overload caused by social media is negatively related to students’ mental well-being, it contributes to emotional coping in a positive sense.
Conclusions
Information overload due to social media usage accounts for risk perception on both a personal-level and a societal-level, which encourages students’ adoption of emotional coping strategies, thus leading to predictions of students’ psychological capital. This study identifies a chain mediated model structured by variables including information overload, risk perception, emotional coping, and psychological capital of university students during the 2022 outbreak in Shanghai.
Preparation and Characterization of FTO/nZnO/CH3NH3PbI3/C/Cu Perovskites Solar Cell
Authors: ElMohamady Noha , Taher Fatema abdel-Rahman , El-sayed Galila M , Elshikh Salah , Mahdy Iman A.
Published: August 2023

Nano ZnO is prepared by the sol-gel method and acts as a conductive seed layer in perovskite solar cells. Methylamine lead iodide CH3NH3PbI3 (MAPbI3) was prepared by two steps method of methylamine iodide CH3NH3I/nZnO/FTIO glass and nPbI2. The effect of ZnO semiconductors nanostructure was studied due to its physical properties arising from quantum confinement. Also, it is considered problematic for Perovskite solar-cells due to the notorious instability of the nZnO/perovskite interface, particularly in the case of using MAPbI3 and its effect on the cell efficiency. The nanostructure is characterized using different techniques. The nZnO was introduced into the Perovskite solar cells structure to improve the electron extraction efficiency of Perovskite solar cells, and to increase the stability of Perovskite solar cells using Commercial Carbon is used as whole transparent material. The current-voltage curves of the FTO/nZnO/CH3NH3PbI3/C/Cu solar cell with a perovskite structure have high conversion efficiencies and stability η = 80.41%.
TRIM2, S100A14, CYP4B1, LMO7, and SFN are signature genes in the progression of idiopathic pulmonary fibrosis epithelial cells to their lung adenocarcinoma comorbidities: a comprehensive analysis based on single-cell RNA sequencing data and RNA transcriptome data
Authors: Zhou Xianqiang , Tan Fang , Zhang Tiansong
Published: August 2023

Background
Patients with idiopathic pulmonary fibrosis (IPF) have a significantly higher prevalence of lung adenocarcinoma (LUAD) than normal subjects, but the underlying association is unclear. This study aims to reveal the potential mechanisms by which IPF progresses to LUAD comorbidity.
Methods
The raw data involved in the study were obtained from the Gene Expression Omnibus (GEO) database. Sc-RNA seq data were applied to identify specific cell types for disease. Differential expression analysis and Weighted Gene Co-Expression Network Analysis (WGCNA) were used to screen for differentially expressed genes (DEGs) and modular signature genes (MSGs) shared by IPF and LUAD. Genes intersecting DEGs and MSGs were considered hub genes. After performing 3 kinds of machine learning algorithms on epithelial cell-derived hub genes, we captured epithelial cell-derived signature genes (EDSGs) shared. External cohort data were exploited to validate the robustness of EDSGs. We adopted immunohistochemical staining to reveal the protein expression levels of EDSGs. K-M plots denoted the survival prognosis of EDSGs in LUAD patients. Based on EDSGs, we constructed a TF-Gene-miRNA regulatory network and predicted the candidate drugs. Molecular docking can validate the strength of action between candidate drugs and EDSGs.
Results
Epithelial cells were a specific cell type shared by IPF and LUAD. And then we obtained 650 DEGs and 1773 MSGs, which were shared by IPF and LUAD. As for 379 hub genes, we performed pathway and functional enrichment analysis, aiming to explore the molecular mechanisms shared. By analyzing sc-RNA seq data, we identified 1234 marker genes of IPF epithelial cell-derived and 1481 of LUAD. And these epithelial cell-derived marker genes shared 8 genes with 379 hub genes. Furthermore, through the machine learning algorithm, we got 5 EDSGs, including TRIM2, S100A14, CYP4B1, LMO7, and SFN. The ROC curves validated that EDSGs have high accuracy in predicting the onset of LUAD comorbidity in IPF patients. The TF-Gene-miRNA network revealed potential regulatory mechanisms of EDSGs. And molecular docking suggested strong binding activity between Thalidomide, Docetaxel, and Imatinib and their corresponding targets.
Conclusion
Our study preliminarily identified potential mechanisms for the progression of IPF to its LUAD comorbidity, which will inform subsequent studies.
Development and Evaluations of the Broadband Ocean Bottom Seismometer (Yardbird-BB OBS) in Taiwan.
Authors: Ching-ren Lin , Liao Yi-Chun , Kuo Ban-Yuan , Wang Chau-chang , Chen Hsin-Hung , Jang Jia-pu , Chen Po-chi , Lin Feng-Sheng , Chang Kun-Hui , Chang Hsu-kuang
Published: August 2023

This study focuses on the development and evaluation of the Broadband Ocean Bottom Seismometer (called Yardbird-BB OBS) in Taiwan. The Yardbird-BB OBS is a critical instrument used to recording seismic signals in deep-sea environments. Rigorous testing and evaluation methods are employed to ensure optimal performance and data recording capabilities. Components such as the data logger, seismic sensor leveling mechanism, acoustic device, and internal wiring undergo thorough performance evaluations prior to assembly. This guarantees the selection of high-performance components and verifies their functionality, preventing issues like data loss or incomplete recording. Once assembled, the Yardbird-BB OBS undergoes a deployment test in the deep sea, typically lasting 3–6 months, to record seismic signals from various sources worldwide. Recorded data from deployments in the Okinawa Trough in 2016 and 2017 are analyzed, highlighting significant seismic events such as a magnitude 7.8 earthquake in New Zealand and a magnitude 6.3 earthquake resulting from a nuclear test by North Korea. The OBS's performance is assessed through waveform analysis, specifically focusing on P-wave arrival times and waveform quality. The results showcase successful automatic leveling adjustment for the seismometer. These high-quality data recordings have valuable research applications, including the study of plate tectonics, estimation of crustal ages, determination of seafloor ambient noise, and improvement of earthquake location accuracy. Additionally, the study presents methods for verifying instrumental self-noise, dynamic range, digitization sensitivity, linearity error, clock drift, and power consumption of the data logger. Calibration procedures and evaluation methods provide valuable insights into the performance characteristics of the Yardbird-BB OBS. The comprehensive evaluations and testing methods outlined in this study contribute to a better understanding and enhancement of the Yardbird-BB OBS, enabling its effective deployment for long-term underwater data recording and valuable scientific research.
Transcription factor TFAP2B exerts neuroprotective effects targeting BNIP3 mediated mitophagy in ischemia/ reperfusion injury
Authors: Peng Yong , Jia Jiaoying , Zhang Mingming , Ma Wenjia , Cui Yan , Yu Mengqiang
Published: August 2023

Cerebral ischemia-reperfusion injury (CIRI) leads to malignant brain edema, blood-brain barrier destruction and neuronal apoptosis. N6-methyladenosine (m6A) RNA modification in CIRI has still limited. MeRIP and RNA-sequencing were performed of middle cerebral artery occlusion and reperfusion (MCAO/R) rats to find novel potential molecular targets. Transcription factor TFAP2B, was standed out of which its m6A abundance decreased associated with a marked reduction of its mRNA based on joint interactive bioinformatics analysis of the MeRIP and RNA-Sequencing data. It was suggeted TFAP2B could have an role in CIRI. Functionally, overexpression of TFAP2B in cultrued primary neurons could effectively improve the cell survival and pro-survival autophagy in parallel with reduced cell apoptosis during OGD/R in vitro. Through the RNA-sequencing of TFAP2B overexpressed primary neurons and subsequent validation experiments, it was found that mitophagy receptor BNIP3 was one of an important target of TFAP2B in OGD/R neurons through which TFAP2B could bind to its promoter region for further transcriptional activation of BNIP3, thereby enhancing BNIP3 mediated mitophagy to protect against OGD/R injury of neurons. Lastly, TFAP2B was demonstrated to alleviated the MCAO/R damage to a certain extent in vivo. Although it was failed to confirm TFAP2B dysregulation was m6A dependent in current research, this is the first study of TFAP2B in CIRI field with important guiding significance.
An inquiry into the drivers of an entrepreneurial economy: A Bayesian clustering approach
Authors: Camacho Maximo , Congregado Emilio , Rodriguez-Santiago Ana
Published: August 2023

Understanding the worldwide drivers of qualified entrepreneurship is a key issue in economic policy design. To help policy decisions exert their intended impact, we aim to cluster a wide range of countries on the basis of their levels and trends in self-employment productivity using a finite mixture model applied to a new large dataset of 121 countries covering the period of 1991–2019. Our results point to three groups of high-, medium-, and low-productive means and tendencies, the geographical distribution of which suggests that they can be reinterpreted using the three stages of economic development, namely, innovation-, efficiency-, and factor-driven economies. Notably, we find that widespread digitalization and low unemployment enhance the probability of transitioning into a highly productive cluster. However, we failed to find that industry weight or employment protection legislation strictness serve as determinants in the transition between groups. Suggestive rationales for these results and implications for the entrepreneurship policy agenda are also provided.
JEL codes: M13; J24; C11; O43
Comparison of the Diagnostic Significance of CSF Metagenomic Next-generation Sequencing Copy Number Variation Analysis and Cytology in Leptomeningeal Malignancy
Authors: Zhang Le , Fang Kechi , Ren Haitao , Fan Siyuan , Wang Jing , Guan Hongzhi
Published: August 2023

Purpose
The early diagnosis of leptomeningeal malignancy remains a formidable challenge in clinical practice. This study aimed to investigate the diagnostic potential of CSF metagenomic next-generation sequencing (mNGS) and chromosome copy number variations (CNVs) analysis in the detection of leptomeningeal malignancy. The diagnostic significance of mNGS-CNVs was compared with that of cytology.
Methods
A total of 51 patients were enrolled. 34 patients were diagnosed with central nervous system (CNS) leptomeningeal malignancy (tumor group), and 17 patients were diagnosed with CNS inflammatory diseases (nontumor group). We explored a well-designed approach utilizing the CSF mNGS-CNVs technique for the early diagnosis of leptomeningeal malignancy. The diagnostic performance of CSF cytology and mNGS-CNVs was evaluated.
Results
CSF cytology displayed a sensitivity of 82.35% (95% CI: 66.83%-92.61%) and a specificity of 94.12% (95% CI: 69.24%-99.69%). In comparison, CSF mNGS-CNVs exhibited a slightly lower sensitivity of 70.59% (95% CI: 52.33%-84.29%), but an impressive specificity of 100% (95% CI: 77.08%-100%). Notably, comparative analysis revealed no significant difference in diagnostic consistency between cytology and mNGS-CNVs. However, mNGS-CNVs exhibited a higher specificity than cytology.
Conclusion
Our study highlighted the advantage of CSF mNGS-CNVs as a diagnostic tool for leptomeningeal malignancy when compared to traditional cytology. This comprehensive approach provides a promising strategy for utilizing CSF mNGS in the detection of CNS tumors.
Characteristics and complete genome analysis of pathogenic Aeromonas veronii from diseased Siniperca chuatsi
Authors: Zhu Xinhai , Qin Lijie , Zhu Yujie , Qian Qieqi , Gao Xiaojian , Jiang Qun , Wang Jun , Liu Guoxing , Zhang Xiaojun
Published: August 2023

As an opportunistic pathogen, Aeromonas veronii can cause hemorrhagic septicemia of various aquatic animals. In our present study, a dominant strain SJ4 isolated from naturally infected mandarin fish (Siniperca chuatsi), was identified as A. veronii according to the morphological, physiological, and biochemical features, as well as molecular identification. Intraperitoneal injection of A. veronii SJ4 into S. chuatsi fingerlings revealed clinical signs similar to the natural infection, and the median lethal dosage (LD50) of the SJ4 to S. chuatsi was 3.8×105 CFU/mL. Histopathological analysis revealed that the isolate SJ4 could cause cell enlargement, obvious hemorrhage and inflammatory responses in S. chuatsi. Detection of virulence genes showed the isolate SJ4 carried act, fim, flgM, ompA, lip, hly, aer, eprCAL and the isolate SJ4 also produce caseinase, dnase, gelatinase, and hemolysin. In addition, the complete genome of A. veronii SJ4 was sequenced, and the size of the genome of A. veronii SJ4 was 4562694 bp, within a G + C content of 58.95%, containing 4079 coding genes. 910 genes encoding for several virulence factors such as type III and VI secretion system, flagella, motility, etc., were determined based on the VFDB database. Besides, 148 antibiotic resistance-related genes in 27 categories related to tetracyclines, fluoroquinolones, aminoglycosides, macrolides, chloramphenicol, and cephalosporins were also annotated. The present results suggested that A. veronii was etiological agent causing the bacterial septicemia of S. chuatsi in this time, as well as provided a valuable base for revealing pathogenesis and resistance mechanism of A. veronii.
SQSTM1/p62 is a prognostic molecular marker and potential therapeutic target for pancreatic neuroendocrine tumours
Authors: Song Yu-Li , Weng Jun-Hua , Zhao Da-Chun , Zhang Jia-Lei , Chen Yuan-Jia , Xu Baohong
Published: August 2023

Background
There have been few studies on the role of autophagy in pancreatic neuroendocrine tumours (PNETs). SQSTM1/p62 (also called Sequestosome 1) is a potential autophagy regulator, and its biological roles and clinical significance in PNETs remain poorly understood.
Purpose
The purpose of this study was to evaluate the clinical significance of SQSTM1/p62 in human PNET specimens and to evaluate its potential value as a therapeutic target by studying its biological function in PNET cell lines.
Methods
SQSTM1/p62 protein expression was assessed in 106 PNET patient specimens by immunohistochemistry, and the relationship between SQSTM1/p62 protein expression and the clinicopathological features of PNETs in patients was analysed. The proliferation, invasion and apoptosis of SQSTM1/p62-knockdown QGP-1 and INS-1 cells were assessed by the MTT assay, a Transwell assay and flow cytometry. Cell autophagy was assessed by western blotting and mCherry-GFP-LC3B.
Results
The protein expression of SQSTM1/p62 in PNET patient specimens was significantly correlated with tumour recurrence (p = 0.005) and worse prognosis (log rank p = 0.020). Downregulation of the SQSTM1/p62 gene inhibited tumour cell proliferation and migration and induced PNET cell death. Downregulation of SQSTM1/p62 activated autophagy in PNET cell lines but blocked autophagic flow. Knockdown of the SQSTM1/p62 gene inhibited mTOR phosphorylation.
Conclusion
The SQSTM1/P62 protein could be an independent prognostic marker for PNET patients. Downregulating SQSTM1/P62 can inhibit PNET progression, inhibit mTOR phosphorylation and block autophagic flow.
To hatch and hatch not: does heterochrony in onset of vestibular mechanosensing explain species differences in escape-hatching success of Agalychnis embryos in snake attacks?
Authors: Güell Brandon André , Warkentin Karen M.
Published: August 2023

Phyllomedusid treefrogs hatch prematurely to escape egg predation, but escape success varies among species. Snake attacks elicited 55% less escape-hatching in spontaneously hatching Agalychnis spurrelli than in less developed A. callidryas. Agalychnis callidryas use their vestibular system and, secondarily, their lateral line to sense physical disturbances that cue hatching. Since A. spurrelli develop faster, we hypothesized that heterochronic shifts in the onset timing of vestibular mechanosensory function, relative to hatching ability, might explain their lower escape response to mechanosensory cues. To test this, we compared onset timings of hypoxia- and mechanosensory-cued hatching (MCH) and vestibular mechanosensory function in developmental series of both species. Across species, most sibships began responding to each cue at the same developmental stage and vestibular function development, measured by the vestibulo-ocular reflex (VOR), was similar. These results demonstrate that low escape-hatching success in A. spurrelli is not caused by a delay in the onset of vestibular mechanosensory function. MCH onset in A. spurrelli was associated with VOR, but with a higher threshold than in A. callidryas. The absence of MCH before strong vestibular function suggests multimodal mechanosensing may not contribute to antipredator responses of A. spurrelli embryos. Our observations of higher escape success of larger clutches in snake attacks and hatching complications in flooded A. spurrelli suggest that differences in clutch and egg capsule structure may contribute directly to species differences in escape-hatching success. Moreover, hatching complications in A. spurrelli may select against false alarms, increasing the stringency of hatching decision rules.
Adaptive Location Method for Film Cooling Holes Based on The Design Intent of The Turbine Blade
Authors: Hou Yaohua , Wang Jing , Mei Jiawei , Zhao Hualong
Published: August 2023

Due to the inevitable deviation of the casting process, the dimensional error of the turbine blade is introduced. As a result, the location datum of the film cooling holes is changed, which has an impact on the machining accuracy. The majority of pertinent studies concentrate on the rigid location approach for the entire blade, which results in a modest relative position error of the blade surface but still fails to give the exact position and axial direction of the film cooling holes of the deformed blade. In this paper, the entire deformation of the blade cross-section curve is divided into a number of deformation combinations of the mean line curve based on the construction method of the blade design intent. The exact location of the film cooling holes in the turbine blade with deviation is therefore efficiently solved by a flexible deformation of the blade that optimizes the position and axial direction of the holes. The verification demonstrates that the novel method can significantly reduce both the contour deviation of the blade surface and the location issue of the film cooling holes. After machining experiments, the maximum position deviation of the holes is reduced by approximately 80% compared to the rigid location method of the entire blade, and the average value and standard deviation are also decreased by about 70%.
Research on the generalization ability of frequency reduction multiplexing for the fall detection algorithm
Authors: Wang Jinghua , Liu Yadong , Qiao Xingshu , Liu Xiaoliang , Zhao Xin
Published: August 2023

The global healthcare coverage systems are actively addressing the issue of patient falls in hospital rooms. To reduce the injury caused by falls and improve the quality of patient care. In this paper, the fall detection system based on the wrist wearable device is studied. The original acceleration data collected by the wearable device is processed by the filtering algorithm to make the corresponding data set. In the face of the sample imbalance problem caused by the small number of fall samples, the time series fragments of fall detection are determined by the event detection mechanism, and the diversity of sample features is increased by reducing the frequency of the original data and re-extracting the features, which improves the performance of the fall detection model. we explored the relationship between sampling frequency and the performance of the fall detection algorithm and found that the model based on low-frequency fall data has a stronger generalization ability. The system realized the classification of 4 fall behaviors such as standing fall and bed fall and 11 daily behaviors such as walking, side-by-side lifting, and leg-clapping, and achieved an average accuracy of 99.02% and a sensitivity of 98.99% in the leave-one-subject cross-validation
Prevalence and trends of brain tumors in a national referral hospital in Nairobi, Kenya
Authors: Gesaka Samwel Rema , Okemwa Parmenas Minda
Published: August 2023

Purpose: To determine the prevalence of brain tumors at a national referral hospital in Nairobi, Kenya.
Methods: This retrospective study retrieved patient-archived records between 2016-2019 at Kenyatta National Hospital. The prevalence of brain tumors was calculated according to the histological types, age, and sex using the Statistical Package for Social Sciences version 24 (IBM Corp., Armonk, NY, USA) and presented in tables and figures.
Results: The average age of all patients diagnosed with brain tumors was 34.43 years. Meningiomas and glioblastomas were the most diagnosed benign and malignant tumors accounting for 36% and 15.5% of cases, respectively. Meningiomas were more common in women, whereas gliomas were more common in men. The combined prevalence rates of brain tumors in children and adolescents (0-19 years) were 28.16 % and 71.84% in adults.
Conclusion: The number of histologically diagnosed brain tumors appeared to increase steadily over the study period. The number of diagnosed cases also increased with increasing patient age, peaking between 35-70 years, and more females compared than males were diagnosed with brain tumors.
Doxycycline reversal of amphetamine-induced mania-like behavior is related to adjusting brain monoamine abnormalities and antioxidant effects in primary hippocampal neurons.
Authors: Chaves-Filho Adriano José Maia , Soares Michele Verde-Ramo , Juca Paloma Marinho , Oliveira Tatiana de Queiroz , Clemente Dino Cesar da Silva , Monteiro Carlos Eduardo da Silva , Silva Francisca Géssica Oliveira , de Aquino Pedro Everson Alexandre , Macedo Danielle S.
Published: August 2023

Purpose: Mania is associated with disturbed dopaminergic transmission in frontotemporal regions. D-amphetamine (AMPH) causes increased extracellular DA levels, considered an acknowledged mania model in rodents. Doxycycline (DOXY) is a second-generation tetracycline with promising neuroprotective properties. Here, we tested the hypothesis that DOXY alone or combined with Lithium (Li) could reverse AMPH-induced mania-like behavioral alterations in mice by the modulation of monoamine levels in brain areas related to mood regulation, as well as cytoprotective and antioxidant effects in hippocampal neurons.
Methods: Male Swiss mice received AMPH or saline intraperitoneal (IP) injections for 14 days. Between days 8-14, mice receive further IP doses of DOXY, Li, or their combination. For in vitro studies, we exposed hippocampal neurons to DOXY in the presence or absence of AMPH. Results: DOXY alone or combined with Li reversed AMPH-induced risk-taking behavior and hyperlocomotion. DOXY also reversed AMPH-induced hippocampal and striatal hyperdopaminergia. In AMPH-exposed hippocampal neurons, DOXY alone and combined with Li presented cytoprotective and antioxidant effects, while DOXY+Li also increased the expression of phospho-Ser133-CREB. Conclusion: our results add novel evidence for DOXY’s ability the reversal of mania-like features while revealing that antidopaminergic activity in some brain areas, such as the hippocampus and striatum, as well as hippocampal cytoprotective effects may account for this drug’s antimanic action. This study provides additional rationale for designing clinical trials investigating its potential as a mood stabilizer agent.