bluuebunny/crossref_metadata_2025 huggingface dataset:
Penyuluhan Perilaku CERDIK dan PATUH sebagai Upaya Penanggulangan Hipertensi pada Lansia di Desa Tonasa, Takalar
Authors: Sari Nurmala , Dewi Laela Alfionita , Rafliansyah Rafliansyah , Ramadani Asifah Baiq , Zainuddin Fatimah Azzahrah , Marzuki Maisya Finistri , Syam Dian Fahira , Assyarifah Khairunnisa
Published: January 2023

Non-communicable diseases are one of the leading causes of death in the world. Hypertension is one of the trigger factors for the occurrence of several non-communicable diseases that are generally experienced by the elderly. Preventive measures are needed in this case, knowledge also affects this so it is necessary to hold counseling. The purpose of this counseling is to educate the target regarding CERDIK and PATUH behavior as an effort to prevent and control hypertension in the elderly. The service is carried out through several stages, namely the planning stage, the implementation stage and the evaluation stage. The implementation of counseling uses the help of communication media in the form of posters. While the evaluation of the extension was assisted by pre-post test questionnaires which were distributed to respondents. The target in this counseling is the elderly as many as 14 people in Tonasa Village. The value of the results of the pre-post test was then analyzed using the Wilcoxon test. The test results show an increase in the average knowledge of the respondents from 6.9 to 8.9.
Optimalisasi Peran Keluarga dalam Peningkatan Kesadaran Pencegahan dan Deteksi Dini Kanker Serviks
Authors: Nilam Sari Atriany , Nurhidayati Siti , Novika Revi Gama , Maulina Rufidah , Maulida Luluk Fajria , Wahidah Nurul Jannatul
Published: January 2023

Cervical cancer is still the cancer that results in the highest female deaths in Indonesia.. The coverage of early detection of cervical cancer in Indonesia is still 8.29%. It was proven that the screening results in Karanganyar showed that as many as 239 mothers with positive iva tests and 15 mothers were suspected of cervical cancer. The purpose of this community service is to provide health education to the community by maximizing family participation in counseling. The target of this activity is 30 couples at the Mojogedang, Karanganyar. The method carried out includes three stages, namely problem identification, implementation, and evaluation. The result of this community service is to increase awareness to carry out early detection of cervical cancer, and reduce stigma regarding cervical cancer. Health counseling or education activities are expected to be carried out in various places by considering the participation of other family members in counseling
Pemberdayaan Kader Posyandu dalam Program Gerakan Amankan Kehamilan dan Persalinan (GEBRAK) dengan Mobile Application Family Center Maternity Care (FCMC)
Authors: Jayanti Nicky Danur , Mayasari Senditya Indah
Published: January 2023

Efforts to accelerate the success of reducing the Maternal Mortality Rate (MMR) and Infant Mortality Rate (IMR), especially those related to maternal health efforts, are increasing cross-sectoral partnerships as outlined in a high-risk pregnant women's mentoring activity by cadres carried out in Malang City known as the GEBRAK (Movement to Secure Pregnancy and Childbirth) program. "Posyandu Melati" cadre activities such as training or mentoring cannot be carried out regularly scheduled and have not been carried out by all posyandu posts because of several constraints. The purpose of this community service is to increase the participation of the Cadre of Pregnant Women with High Risk Pregnancies and provide training and community empowerment on early detection of High Risk Pregnancies using the FCMC Mobile Application media method in Tasikmadu Village totaling 7 people. Results: three activities, namely Training Activities and mentoring Posyandu Cadres with the topic of high-risk pregnancy, KSPR, and FCMC mobile application, the second activity is socialization and application of the FCMC Mobile Application to pregnant women and their families and communities. The last activity is the Recording and Early Detection of High Risk Pregnancies by Cadres as a support for the success of the GEBRAK program. Increasing the skills of Posyandu cadres about monitoring high-risk pregnancies so that prevention, healing and recovery efforts can be given with clear indications as early as possible during pregnancy. Posyandu cadres are expected to be able to carry out their duties according to agreed commitments and be able to maintain infrastructure as a good support for activities.
Edukasi Penanganan Hipertensi dengan Acupressure di Banjar Dinas Megati Kaja, Provinsi Bali
Authors: Darmawati I Dewa Ayu Agra , Harditya Kadek Buja , Anggaraeni Komang Rosa Tri
Published: January 2023

Since the Covid-19 pandemic, several programs that were routinely carried out had to be stopped, one of which was the free medical treatment which was routinely carried out by the Selemadeg Timur I Public Health Center, Tabanan Regency, Bali. The cessation of free treatment at the Banjars in Selemadeg Timur sub-district, Tabanan Regency - Bali due to the Covid-19 pandemic has made people not carry out routine screening of their illnesses, especially hypertension. with hypertension. The aim is to provide knowledge to sufferers and families about handling hypertension at home. The activity was carried out through the provision of education in the form of providing leaflets about the management of hypertension with acupressure techniques and providing masks to prevent transmission of the corona virus-19 to 70 patriarchs located at Banjar Dinas Megati Kaja, Tabanan, Bali, Indonesia. The method used is through lectures, discussions, and demonstrations. The activity was carried out from 10 September - 12 September 2021. The entire Banjar Dinas Megati Kaja community accepted the assistance provided and the activity implementation ran smoothly. We wish that this activity can continue and become one of the programs of the Village
Upaya Mitigasi dan Adaptasi Perubahan Iklim melalui Media Permainan Anak Atteropoly kepada Anak Usia Sekolah Dasar
Authors: Tumanggor Winni R.E. , Chahaya Indra , Nurhasanah Rossy
Published: January 2023

Bogak Village is a coastal village with a waste problem that can contribute to climate change. Instilling waste management knowledge needs to be done with the appropriate method. The purpose of the activity was to apply the Atteropoly game as an early education means regarding waste management for elementary school-age children in efforts to mitigate and adapt to climate change in Bogak Village. The stages were planning and preparation (gathering information on partner problems and compiling educational game instruments), implementation of service activities, and monitoring evaluation. Educational activities through the Atteropoly game were directly carried out on July 6, 2022 to 30 participants of elementary school-age children and two teachers from the Rumah Qur’an Miftahul Jannah. Measuring the success of Atteropoly media used pre-test and post-test, it was found that there was an increase in participants’ knowledge and the average score of 38,33 (pre-test) to 53,00 (post-test). It can be concluded the Atteropoly game increases the elementary school-aged children’s knowledge in Bogak Village. The community service team also gave a set of Atteropoly game tools and segregated trash bins to the village. It is hoped that the Atteropoly game tool can be used to educate the public, especially elementary school-age children in Bogak Village.
Research progress of hydrogels as delivery systems and scaffolds in the treatment of secondary spinal cord injury
Authors: Peng Haichuan , Liu Yongkang , Xiao Fengfeng , Zhang Limei , Li Wenting , Wang Binghan , Weng Zhijian , Liu Yu , Chen Gang
Published: January 2023

Secondary spinal cord injury (SSCI) is the second stage of spinal cord injury (SCI) and involves vasculature derangement, immune response, inflammatory response, and glial scar formation. Bioactive additives, such as drugs and cells, have been widely used to inhibit the progression of secondary spinal cord injury. However, the delivery and long-term retention of these additives remain a problem to be solved. In recent years, hydrogels have attracted much attention as a popular delivery system for loading cells and drugs for secondary spinal cord injury therapy. After implantation into the site of spinal cord injury, hydrogels can deliver bioactive additives in situ and induce the unidirectional growth of nerve cells as scaffolds. In addition, physical and chemical methods can endow hydrogels with new functions. In this review, we summarize the current state of various hydrogel delivery systems for secondary spinal cord injury treatment. Moreover, functional modifications of these hydrogels for better therapeutic effects are also discussed to provide a comprehensive insight into the application of hydrogels in the treatment of secondary spinal cord injury.
Perinatal mesenchymal stromal cells of the human decidua restore continence in rats with stress urinary incontinence induced by simulated birth trauma and regulate senescence of fibroblasts from women with stress urinary incontinence
Authors: De La Torre Paz , Pérez-Lorenzo María Jesús , Alcázar-Garrido Álvaro , Collado Jennifer , Martínez-López Mario , Forcén Laura , Masero-Casasola Ana R. , García Alicia , Gutiérrez-Vélez Mª Carmen , Medina-Polo José , Muñoz Eloy , Flores Ana I.
Published: January 2023

Stress urinary incontinence (SUI) is a condition that causes the involuntary loss of urine when making small efforts, which seriously affects daily life of people who suffer from it. Women are more affected by this form of incontinence than men, since parity is the main risk factor. Weakening of the pelvic floor tissues is the cause of SUI, although a complete understanding of the cellular and molecular mechanisms of the pathology is still lacking. Reconstructive surgery to strengthen tissue in SUI patients is often associated with complications and/or is ineffective. Mesenchymal stromal cells from the maternal side of the placenta, i.e. the decidua, are proposed here as a therapeutic alternative based on the regenerative potential of mesenchymal cells. The animal model of SUI due to vaginal distention simulating labor has been used, and decidual mesenchymal stromal cell (DMSC) transplantation was effective in preventing a drop in pressure at the leak point in treated animals. Histological analysis of the urethras from DMSC-treated animals after VD showed recovery of the muscle fiber integrity, low or no extracellular matrix (ECM) infiltration and larger elastic fibers near the external urethral sphincter, compared to control animals. Cells isolated from the suburethral connective tissue of SUI patients were characterized as myofibroblasts, based on the expression of several specific genes and proteins, and were shown to achieve premature replicative senescence. Co-culture of SUI myofibroblasts with DMSC via transwell revealed a paracrine interaction between the cells through signals that mediated DMSC migration, SUI myofibroblast proliferation, and modulation of the proinflammatory and ECM-degrading milieu that is characteristic of senescence. In conclusion, DMSC could be an alternative therapeutic option for SUI by counteracting the effects of senescence in damaged pelvic tissue.
Insulin-like peptide 8 (Ilp8) regulates female fecundity in flies
Authors: Li Haomiao , Luo Xi , Li Na , Liu Tao , Zhang Junzheng
Published: January 2023

Introduction: Insulin-like peptides (Ilps) play crucial roles in nearly all life stages of insects. Ilp8 is involved in developmental stability, stress resistance and female fecundity in several insect species, but the underlying mechanisms are not fully understood. Here we report the functional characterization of Ilp8s in three fly species, including Bactrocera dorsalis, Drosophila mercatorum and Drosophila melanogaster.

Methods: Phylogenetic analyses were performed to identify and characterize insect Ilp8s. The amino acid sequences of fly Ilp8s were aligned and the three-dimensional structures of fly Ilp8s were constructed and compared. The tissue specific expression pattern of fly Ilp8s were examined by qRT-PCR. In Bactrocera dorsalis and Drosophila mercatorum, dsRNAs were injected into virgin females to inhibit the expression of Ilp8 and the impacts on female fecundity were examined. In Drosophila melanogaster, the female fecundity of Ilp8 loss-of-function mutant was compared with wild type control flies. The mutant fruit fly strain was also used for sexual behavioral analysis and transcriptomic analysis.

Results: Orthologs of Ilp8s are found in major groups of insects except for the lepidopterans and coleopterans, and Ilp8s are found to be well separated from other Ilps in three fly species. The key motif and the predicted three-dimensional structure of fly Ilp8s are well conserved. Ilp8 are specifically expressed in the ovary and are essential for female fecundity in three fly species. Behavior analysis demonstrates that Ilp8 mutation impairs female sexual attractiveness in fruit fly, which results in decreased mating success and is likely the cause of fecundity reduction. Further transcriptomic analysis indicates that Ilp8 might influence metabolism, immune activity, oocyte development as well as hormone homeostasis to collectively regulate female fecundity in the fruit fly.

Discussion: Our findings support a universal role of insect Ilp8 in female fecundity, and also provide novel clues for understanding the modes of action of Ilp8.
CFD supported scale up of perfusion bioreactors in biopharma
Authors: Kuschel Maike , Wutz Johannes , Salli Mustafa , Monteil Dominique , Wucherpfennig Thomas
Published: January 2023

The robust scale up of perfusion systems requires comparable conditions over all scales to ensure equivalent cell culture performance. As cells in continuous processes circulate outside the bioreactor, performance losses may arise if jet flow and stirring cause a direct connection between perfusion feed and return. Computational fluid dynamics can be used to identify such short circuit flows, assess mixing efficiencies, and eventually adapt the perfusion setup. This study investigates the scale up from a 2 L glass bioreactor to 100 L and 500 L disposable pilot scale systems. Highly resolved Lattice Boltzmann Large Eddy simulations were performed in single phase and mixing efficiencies (Emix) furthermore experimentally validated in the 2 L system. This evaluation gives insight into the flow pattern, the mixing behavior and information on cell residence time inside the bioreactors. No geometric adaptations in the pilot scale systems were necessary as Emix was greater than 90% for all conditions tested. Two different setups were evaluated in 2 L scale where the direction of flow was changed, yielding a difference in mixing efficiency of 10%. Nevertheless, since Emix was confirmed to be >90% also for both 2 L setups and the determined mixing times were in a similar range for all scales, the 2 L system was deemed to be a suitable scale down model. The results demonstrate how computational fluid dynamic models can be used for rational process design of intensified production processes in the biopharmaceutical industry.
Retrieval-induced forgetting in declarative and procedural memory: Inhibitory suppression resolves interference between motor responses
Authors: Buchli Dorothy R.
Published: January 2023

Over a century's worth of research suggests that, for a variety of verbal and perceptual memory phenomena, retrieval modifies memory in two ways. First, retrieved information is strengthened and thus more easily retrieved on subsequent recall tests. Secondly, when information is stored or organized in memory in such a way that multiple representations share a common retrieval cue, and are thus subject to interference, related information that is not retrieved becomes less recallable. Such retrieval-induced forgetting is a highly robust and well-understood phenomenon. Over the past decade, several experiments have demonstrated that retrieval-induced forgetting also persists for motor responses–both simple and complex, and that this impairment is observed for representations stored in both declarative and procedural memory. While several review papers have focused on retrieval-induced forgetting of verbal and perceptual information, to date no literature review has focused exclusively on retrieval-induced forgetting of motor responses or actions. In addition, while retrieval-induced forgetting has been documented extensively within the domain of declarative memory, only a few papers have been published documenting retrieval-induced forgetting in procedural memory. Therefore, this review paper will explore these findings and their primary implications, most notably that when multiple motor programs are initiated via a shared retrieval cue, inhibitory suppression is recruited to decrease accessibility of related, but inappropriate actions in service of potentiating retrieval of appropriate actions. More broadly, the same processes that govern selective retrieval in cognition are implicated in the selective retrieval of motor actions.
Location, location, location: Fibrin, cells, and fibrinolytic factors in thrombi
Authors: Narwal Anuj , Whyte Claire S. , Mutch Nicola J.
Published: January 2023

Thrombi are heterogenous in nature with composition and structure being dictated by the site of formation, initiating stimuli, shear stress, and cellular influences. Arterial thrombi are historically associated with high platelet content and more tightly packed fibrin, reflecting the shear stress in these vessels. In contrast, venous thrombi are generally erythrocyte and fibrin-rich with reduced platelet contribution. However, these conventional views on the composition of thrombi in divergent vascular beds have shifted in recent years, largely due to recent advances in thromboectomy and high-resolution imaging. Interestingly, the distribution of fibrinolytic proteins within thrombi is directly influenced by the cellular composition and vascular bed. This in turn influences the susceptibility of thrombi to proteolytic degradation. Our current knowledge of thrombus composition and its impact on resistance to thrombolytic therapy and success of thrombectomy is advancing, but nonetheless in its infancy. We require a deeper understanding of thrombus architecture and the downstream influence on fibrinolytic susceptibility. Ultimately, this will aid in a stratified and targeted approach to tailored antithrombotic strategies in patients with various thromboembolic diseases.
Modifying effect of the serum level of brain-derived neurotrophic factor (BDNF) on the association between BDNF methylation and long-term cardiovascular outcomes in patients with acute coronary syndrome
Authors: Choi Wonsuk , Kang Hee-Ju , Kim Ju-Wan , Kim Hee Kyung , Kang Ho-Cheol , Kim Sung-Wan , Kim Jung-Chul , Ahn Youngkeun , Jeong Myung Ho , Kim Jae-Min
Published: January 2023

Introduction

This study investigated the potential modifying effects of the serum brain-derived neurotrophic factor (sBDNF) level on the association between BDNF methylation status and long-term cardiovascular outcomes in acute coronary syndrome (ACS) patients.

Methods

From 2006 to 2012, hospitalized ACS patients were consecutively recruited. The sBDNF level and BDNF methylation status were assessed at baseline in 969 patients who were followed up for major adverse cardiac events (MACEs) over 5–12 years, until 2017 or death. Cox proportional hazards models were utilized to compare the time to first composite or individual MACEs between individuals with lower and those with higher average BDNF methylation levels in the low and high sBDNF groups, respectively. The modifying effects of the sBDNF and average BDNF methylation levels on first composite and individual MACEs were analyzed using Cox proportional hazards models after adjusting for potential covariates.

Results

In the low sBDNF group, a higher average BDNF methylation level was linked to an increase in composite MACEs independent of confounding variables, but not in the high sBDNF group [HR (95 percent CI) = 1.04 (0.76–1.44)]. The interaction effect between the sBDNF and average BDNF methylation levels on composite MACEs was significant after adjusting for covariates (P = 0.008).

Conclusion

Combining the BDNF methylation status and sBDNF levels may help identify ACS patients who are likely to have unfavorable clinical outcomes.
Energy metabolism disorder dictates chronic hypoxia damage in heart defect with tetralogy of fallot
Authors: Liu Libao , Huang Lei , Yao Lishuai , Zou Fan , He Jinyuan , Zhao Xiaodong , Mei Lugang , Huang Shuai
Published: January 2023

Background

Tetralogy of Fallot (TOF) belongs to cyanotic heart damage, which is the most common in clinic. In the chronic myocardial hypoxia injury related to TOF, the potential molecular mechanism of cardiac energy metabolism remains unclear.

Materials and methods

In our study, microarray transcriptome analysis and metabonomics methods were used to explore the energy metabolism pathway during chronic hypoxia injury. The gene expression omnibus (GEO) dataset GSE132176 was obtained for analyzing the metabolic pathways. The clinical samples (right atrial tissues) of atrial septal defect (ASD) and TOF were analyzed by metabonomics. Next, we screened important pathways and important differential metabolites related to energy metabolism to explore the pathogenesis of TOF.

Results

Gene set enrichment analysis (GSEA) indicated that fructose 6-phosphate metabolic process, triglyceride metabolic process, and et al. were significantly enriched. Gene set variation analysis (GSVA) results showed that significant difference of ASD group and TOF group existed in terpenoid metabolic process and positive regulation of triglyceride metabolic process. Pathways with significant enrichment (impact > 0.1) in TOF were caffeine metabolism (impact = 0.69), sphingolipid metabolism (impact = 0.46), glycerophospholipid metabolism (impact = 0.26), tryptophan metabolism (impact = 0.24), galactose metabolism (impact = 0.11). Pathways with significant enrichment (impact > 0.1) in ASD are caffeine metabolism (impact = 0.69), riboflavin metabolism (impact = 0.5), alanine, aspartate and glutamate metabolism (impact = 0.35), histidine metabolism (impact = 0.34) and et al.

Conclusion

Disturbed energy metabolism occurs in patients with TOF or ASD, and further investigation was needed to further clarify mechanism.
Meta-analysis of extracorporeal membrane oxygenation in combination with intra-aortic balloon pump vs. extracorporeal membrane oxygenation only in patients with cardiogenic shock due to acute myocardial infarction
Authors: Meertens Max M. , Tichelbäcker Tobias , Macherey-Meyer Sascha , Heyne Sebastian , Braumann Simon , Nießen Stephan F. , Baldus Stephan , Adler Christoph , Lee Samuel
Published: January 2023

Background

Incidence and mortality of cardiogenic shock (CS) in patients with acute myocardial infarction (AMI) remain high despite substantial therapy improvements in acute percutaneous coronary intervention over the last decades. Unloading the left ventricle in patients with Veno-arterial extracorporeal membrane oxygenation (VA-ECMO) can be performed by using an intra-aortic balloon pumps’ (IABP) afterload reduction, which might be especially beneficial in AMI patients with CS.

Objective

The objective of this meta-analysis was to assess the effect of VA-ECMO + IABP vs. VA-ECMO treatment on the mortality of patients with CS due to AMI.

Methods

A systematic literature search was performed using EMBASE, COCHRANE, and MEDLINE databases. Studies comparing the effect of VA-ECMO + IABP vs. VA-ECMO on mortality of patients with AMI were included. Meta-analyses were performed to analyze the effect of the chosen treatment on 30-day/in-hospital mortality.

Results

Twelve studies were identified by the literature search, including a total of 5,063 patients, 81.5% were male and the mean age was 65.9 years. One thousand one hundred and thirty-six patients received treatment with VA-ECMO in combination with IABP and 2,964 patients received VA-ECMO treatment only. The performed meta-analysis showed decreased mortality at 30-days/in-hospital after VA-ECMO + IABP compared to VA-ECMO only for patients with cardiogenic shock after AMI (OR 0.36, 95% CI 0.30–0.44, P≤0.001). Combination of VA-ECMO + IABP was associated with higher rates of weaning success (OR 0.29, 95% CI 0.16–0.53, P < 0.001) without an increase of vascular access complications (OR 0.85, 95% CI 0.35–2.08, P = 0.72).

Conclusion

In this meta-analysis, combination therapy of VA-ECMO + IABP was superior to VA-ECMO only therapy in patients with CS due to AMI. In the absence of randomized data, these results are hypothesis generating only.
Study of nonlinear flow mechanisms and microfracture networks in low-permeability reservoirs
Authors: Hao Mingqiang , Liu Xuewei , Xia Jing , Liu Yang
Published: January 2023

As efficient technologies boost oil yields and economic benefits, horizontal wells and hydraulic fracturing are widely used in low- permeability reservoirs. To better evaluate the reserve and improve recovery, it is essential to determine fluid flow patterns and transport mechanisms. Laboratory experiments, field operations, and analytical studies have identified nonlinear flow and microfracture networks during the fluid flow in a reservoir with fractured horizontal wells. However, the interactions between nonlinear flow and microfracture networks are still not fully understood. In this study, nonlinear flow experiments and triaxial compression tests were carried out to analyze nonlinear flow characteristics in the vicinity of microfracture networks. By analyzing the effects of microfracture networks on nonlinear flow, two-phase flow, rock stress sensitivity, and artificial fractures, we found that fluid capacity in capillaries with smaller dimensions decreased along with a drop in the pressure gradient, generating a nonlinear flow pattern. The area of nonlinear flow was diminished by the presence of microfractures, which improved flow efficiency and reservoir quality. Considering the size of fracture apertures, microfractures behave more like matrix pores than natural fractures. Also, microfractures significantly increase rock stress sensitivity and reduce the threshold permeability, which enhances fluid flow capacity. This study contributes to our understanding of flow behavior, predicting production and improving recovery in low-permeability reservoirs.
Constrained shuffled complex evolution algorithm and its application in the automatic calibration of Xinanjiang model
Authors: Jiang Chenkai , Zhang Silong , Xie Yundong
Published: January 2023

The Shuffled Complex Evolution—University of Arizona (SCE-UA) is a classical algorithm in the field of hydrology and water resources, but it cannot solve constrained optimization problems directly. Using penalty functions has been the preferred method to handle constraints, but the appropriate selection of penalty parameters and penalty functions can be challenging. To enhance the universality of the SCE-UA, we propose the Constrained Shuffled Complex Evolution Algorithm (CSCE) to conveniently and effectively solve inequality-constrained optimization problems. Its performance is compared with the SCE-UA using the adaptive penalty function (SCEA) on 14 test problems with inequality constraints. It is further compared with seven other algorithms on two test problems with low success rates. To demonstrate its effect in hydrologic model calibration, the CSCE is applied to the parameter optimization of the Xinanjiang (XAJ) model under synthetic data and observed data. The results indicate that the CSCE is more advantageous than the SCEA in terms of the success rate, stability, feasible rate, and convergence speed. It can guarantee the feasibility of the solution and avoid the problem of deep soil tension water capacity (WDM)<0 in the optimization process of the XAJ model. In the case of synthetic data, the CSCE can accurately find the theoretical optimal parameters of the XAJ model under the given constraints. In the case of observed data, the XAJ model optimized by the CSCE can effectively simulate the hourly rainfall-runoff events of the Hexi Basin and achieves mean Nash efficiency coefficients greater than 0.75 in the calibration period and the validation period.
Views of secondary education teachers on the use of mixed reality
Authors: Marín-Díaz Verónica , Sampedro-Requena Begoña Esther
Published: January 2023

The advance of the so-called emergent technologies in the field of education goes hand in hand with the previous experiences and beliefs of teachers, or lack thereof, with and about them. Among all the digital resources available, Mixed Reality (MR) is currently awakening the interest of educators, given that it combines virtual and augmented reality. Although both of these technologies are already present in many mixed methodologies utilized for teaching and learning processes, this is not the case of MR. Thus, it is necessary to discover the perspectives of educators on the use of MR, to be able to forecast its successful implementation in classrooms. Thus, the present article shows data obtained from a study with 219 Secondary Education pre-service teachers in Spain. The data collected through a 31-item ad hoc questionnaire pointed to differences in the perception of Mixed Reality in the teaching process as a function of gender, with women considering that it will make the classroom methodology more communicative, also believing that it will promote the reading comprehension of the texts that are utilized in each school subject. Thus, we can conclude that mixed reality is defined as a tool that promotes the learning process of secondary school students.
Novel HYDIN variants associated with male infertility in two Chinese families
Authors: Yu Hui , Shi Xiao , Shao Zhongmei , Geng Hao , Guo Senzhao , Li Kuokuo , Gu Meng , Xu Chuan , Gao Yang , Tan Qing , Duan Zongliu , Wu Huan , Hua Rong , Guo Rui , Wei Zhaolian , Zhou Ping , Cao Yunxia , He Xiaojin , Li Liang , Zhang Xiaoping , Lv Mingrong
Published: January 2023

Introduction

Infertility is a major disease affecting human life and health, among which male factors account for about half. Asthenoteratozoospermia accounts for the majority of male infertility. High-throughput sequencing techniques have identified numerous variants in genes responsible for asthenoteratozoospermia; however, its etiology still needs to be studied.

Method

In this study, we performed whole-exome sequencing on samples from 375 patients with asthenoteratozoospermia and identified two HYDIN compound heterozygous variants, a primary ciliary dyskinesia (PCD)-associated gene, in two unrelated subjects. H&E staining, SEM were employed to analyze the varies on sperm of patients, further, TEM was employed to determine the ultrastructure defects. And westernblot and immunostaining were chose to evaluate the variation of structural protein. ICSI was applied to assist the mutational patient to achieve offspring.

Result

We identified two HYDIN compound heterozygous variants. Patient AY078 had novel compound heterozygous splice variants (c.5969-2A>G, c.6316+1G>A), altering the consensus splice acceptor site of HYDIN. He was diagnosed with male infertility and PCD, presenting with decreased sperm progressive motility and morphological abnormalities, and bronchial dilatation in the inferior lobe. Compared to the fertile control, HYDIN levels, acrosome and centrosome markers (ACTL7A, ACROSIN, PLCζ1, and Centrin1), and flagella components (TOMM20, SEPT4, SPEF2, SPAG6, and RSPHs) were significantly reduced in HYDIN-deficient patients. Using intracytoplasmic sperm injection (ICSI), the patient successfully achieved clinical pregnancy. AY079 had deleterious compound heterozygous missense variants, c.9507C>G (p. Asn3169Lys) and c.14081G>A (p. Arg4694His), presenting with infertility; however, semen samples and PCD examination were unavailable.

Discussion

Our findings provide the first evidence that the loss of HYDIN function causes asthenoteratozoospermia presenting with various defects in the flagella structure and the disassembly of the acrosome and neck. Additionally, ICSI could rescue this failure of insemination caused by immobile and malformed sperm induced by HYDIN deficiency.
Energy consumption and heat island effect mitigation analysis of different roofs considering superposition coupling
Authors: Zhao Shanguo , Zhang Xiaosong
Published: January 2023

Building energy, particularly air conditioning energy, makes for a significant fraction of overall societal energy usage. The heat island effect is a common urban environmental concern that threatens human sustainable development. Roofs, which cover more than 20% of the total metropolitan area, play an essential role in mitigating the urban heat island effect and lowering building energy use. To examine the triple beneficial benefits of cool roofs and green roofs on building energy conservation in different temperature zones in China, a simulation model based on a typical residential community is established. The results reveal that minimizing direct heat acquisition from the roof, reducing heat transfer from the enclosure, and enhancing the coefficient of performance have a surprising triple effect on building energy saving. In cold areas, hot summer and cold winter areas, and hot summer and warm winter areas, cool roofs may lower the regional ambient temperatures by 2°C, 2.3°C, and 2.6°C, respectively, whereas green roofs can reduce the regional ambient temperatures by 1°C, 1.1°C, and 1.2°C. The triple saving impact of cool roofs and green roofs may accomplish 11.0%, 11.5%, 12.6%, and 9.4%, 8.1%, and 9.3%, respectively, for building energy conservation. Because of the increased solar radiation, cool roofs perform better in low-latitude zones, whereas green roofs function consistently.
Low-Carbon and economic flexibility scheduling of power system with multiple generation resources penetration
Authors: Zhang Dawei , Wang Yanfeng , Xi Litang , Deng Fuyao , Deng Zhisen , Liu Jichun , Wei Zhaobin
Published: January 2023

The operation flexibility of the power system suffers great challenges due to the vigorously developing of renewable energy resources under the promotion of the carbon neutralization goal. To this end, this paper proposes an economical and flexible energy scheduling method for power system integrated with multiple generation resources while considering the operation of low-carbon. Specifically, flexibility evaluation indexes are constructed to describe the characteristics of the flexible generation units. Then they are connected with the flexibility of the power system in an economic and low-carbon flexible energy scheduling model. To coordinate the operation economy, flexibility, and carbon emission reduction, the model incorporates demand response, operational characteristics, and flexibility requirements. Further, the model is fully validated through the simulation on the modified IEEE 30-bus system. Results demonstrate that: the proposed method can reduce the system’s carbon emission and total operating costs and promote photovoltaic consumption.
Microplastics in mainstem Mississippi River fishes
Authors: Gad Ahmed K. , Toner Kerrin , Benfield Mark C. , Midway Stephen R.
Published: January 2023

Microplastics are ubiquitous in Earth’s ecosystems and many efforts have begun to understand their distributions. Large rivers, like the Mississippi River, provide a unique system in which to look at large-scale patterns of microplastic distribution. In this study, we sampled four species of widely-distributed fishes from five sites along the mainstem Mississippi River, from Minnesota to Louisiana, United States. Microplastics were found in all fish species and at all sites; however, microplastics increased in occurrence in the Lower Mississippi River. Fragments were the most common morphologies and polypropylene was the most common polymer detected. We also examined the hypothesis that microplastic loads in fishes increased downstream, but found support for this hypothesis only when examining Largemouth Bass; Flathead Catfish, Shortnose Gar, and Bluegill were all found to have similar microplastic loads along the mainstem Mississippi River. It is clear that microplastics are heterogeneously distributed throughout ecosystems, and further understanding of microplastic distributional patterns and varying species burdens are needed to fully understand threats that microplastics present.
Comprehensive assessment of the environmental and employment impacts of international trade from the perspective of global value chains
Authors: Bai Shukuan , Zhang Boya , Ning Yadong
Published: January 2023

A comprehensive assessment of the environmental and employment impacts of international trade is essential for coordinating sustainable development between the economy, environment, and society. International trade is an important driving force for global economic growth. However, the environmental and social impacts of trade under global value chains (GVCs) have not yet been comprehensively characterized. This study aims to estimate the impacts of international trade from 2000–2014 on carbon emissions and employment using the gross export decomposition method, incorporating a counterfactual method based on the multi-region input–output model. We found that (1) at the global level, international trade has been conducive to carbon emission reductions since 2003 and played a role in increasing employment throughout the study period; however, the impacts of GVC-related trade have been mixed, generating global emissions savings, but leading to job losses. This implies that there is a trade-off between carbon emission reduction through GVC embedding and employment creation. (2) At the national level, a country’s participation in international trade benefits the environment, employment, or both. In this sense, international trade is no longer a zero-sum game; rather, it is likely to be a positive-sum game for all participants regarding environmental benefits and job creation. (3) In bilateral trade, developed and developing economies have positive and negative impacts, meaning that they should take joint actions to balance environment–employment–trade growth. These findings are useful for determining the responsibilities of different countries toward climate change and achieving sustainable development of the economy, society, and environment.
How sustainable business model innovation and green technology innovation interact to affect sustainable corporate performance
Authors: Zhou Nan , Pan Liurong , Tian Ye , Zhu Nian , Cai Xiang , Gao Jianhua
Published: January 2023

Sustainable business model innovation (SBMI) and green technology innovation (GTI) are keys to creating shared social and business value, and both are essential elements of responsible innovation. However, SBMI or GTI is unable to develop a sustainable business. The main purpose of this study is to analyze sustainable dual innovation as a conceptual framework based on the panel data of 1468 Chinese manufacturing A-share listed companies from 2010 to 2020, and it has obtained three essential findings. First, there is a significant matching effect between SBMI and GTI on sustainable corporate performance (SCP). Companies report higher levels of SCP when there is a higher congruence in SBMI with GTI. Secondly, organizations with high SBMI-low GTI realize superior SCP compared to organizations with low SBMI-high GTI. The high SBMI-low GTI becomes an ideal choice for most companies and avoids the “destructive factors” brought by low SBMI-high GTI. Finally, a highly aggressive corporate strategy has a “magnifying effect” on the impact of the combination of SBMI and GTI on SCP. The research provides a comprehensive understanding and collective impact of SBMI and GTI on SCP. Overall, findings provide a theoretical basis for enterprises to decide on the consistency/inconsistency trade-off between SBMI and GTI. Moreover, it encourages to enterprises must develop an aggressive strategy to balance.
A morphological guide of neotropical freshwater sponge spicules for paleolimnological studies
Authors: Rasbold Giliane G. , Calheira Ludimila , Domingos-Luz Leandro , Pessenda Luiz C. R. , Pinheiro Ulisses , McGlue Michael M.
Published: January 2023

Freshwater sponges (Porifera: Spongillida) are sessile invertebrates with skeletons composed of siliceous elements termed spicules. Sponge spicules (megascleres, microscleres, and gemmuloscleres) are characterized by widely varying sizes and shapes. These spicules are well-preserved in lacustrine, wetland, and riverine sediments and hold significant ecological and limnological information that can be applied as diagnostic tools in reconstructions of Quaternary environments. However, problems with taxonomy and the absence of systematic guidelines and standards of identification represent major challenges to utilizing freshwater sponges as a paleo-proxy. Here, we present a well-illustrated extraction protocol and morphological guide to the Neotropical freshwater sponge fauna. This guide is intended to introduce researchers and students to the study of freshwater sponges and their use as a diagnostic tool in paleoecology and paleolimnology.
Single-cell RNA-seq reveals cellular heterogeneity from deep fascia in patients with acute compartment syndrome
Authors: Wang Tao , Long Yubin , Ma Lijie , Dong Qi , Li Yiran , Guo Junfei , Jin Lin , Di Luqin , Zhang Yingze , Wang Ling , Hou Zhiyong
Published: January 2023

Introduction

High stress in the compartment surrounded by the deep fascia can cause acute compartment syndrome (ACS) that may result in necrosis of the limbs. The study aims to investigate the cellular heterogeneity of the deep fascia in ACS patients by single-cell RNA sequencing (scRNA-seq).

Methods

We collected deep fascia samples from patients with ACS (high-stress group, HG, n=3) and patients receiving thigh amputation due to osteosarcoma (normal-stress group, NG, n=3). We utilized ultrasound and scanning electron microscopy to observe the morphologic change of the deep fascia, used multiplex staining and multispectral imaging to explore immune cell infiltration, and applied scRNA-seq to investigate the cellular heterogeneity of the deep fascia and to identify differentially expressed genes.

Results

Notably, we identified GZMK+interferon-act CD4 central memory T cells as a specific high-stress compartment subcluster expressing interferon-related genes. Additionally, the changes in the proportions of inflammation-related subclusters, such as the increased proportion of M2 macrophages and decreased proportion of M1 macrophages, may play crucial roles in the balance of pro-inflammatory and anti-inflammatory in the development of ACS. Furthermore, we found that heat shock protein genes were highly expressed but metal ion-related genes (S100 family and metallothionein family) were down-regulated in various subpopulations under high stress.

Conclusions

We identified a high stress-specific subcluster and variations in immune cells and fibroblast subclusters, as well as their differentially expressed genes, in ACS patients. Our findings reveal the functions of the deep fascia in the pathophysiology of ACS, providing new approaches for its treatment and prevention.
Patients with IBD have a more cautious attitude towards COVID-19 vaccination
Authors: Hu Shurong , Liu Jingwen , Li Shuyan , Wu Qiangqiang , Wang Xiaoying , Xu Dingting , Chen Yan
Published: January 2023

Background

To understand the awareness of COVID-19 vaccine, the willingness to vaccinate and the influencing factors of willingness to vaccinate in inflammatory bowel disease (IBD) patients.

Methods

The online questionnaire was distributed to conduct a survey to analyze and evaluate the willingness, awareness and trust in vaccines of IBD patients. Bivariate analyses and logistic regression models were used to analysis influencing factors.

Results

We sent the questionnaire to the WeChat group for patient management and 304 patients responded, out of which 16 respondents had to be excluded and 288 respondents were included for the analysis. Among them, 209 patients vaccinated with COVID-19 vaccine. Among the non-vaccinated 79 patients, the main reasons for their concerns were afraid of vaccination aggravating IBD and fear of adverse effects. Our results showed that IBD patients with long disease duration were more willing to receive COVID-19 vaccination (P<0.05). We also observed that a high perception of benefits and cues to action to receive the vaccine were the two most important constructs affecting a definite intention for COVID-19 vaccination (P<0.05).

Conclusions

Patients with IBD have a more cautious attitude towards COVID-19 vaccination, which may lead to a higher rate of vaccine hesitancy. Further efforts should be made to protect patients with IBD from COVID-19 infections and achieve adequate vaccination coverage.
The genetic and epigenetic regulation of CD55 and its pathway analysis in colon cancer
Authors: Liu Jiawei , Fu Ning , Yang Zhenbang , Li Ang , Wu Hongjiao , Jin Ye , Song Qinqin , Ji Shanshan , Xu Hongxue , Zhang Zhi , Zhang Xuemei
Published: January 2023

Background

CD55 plays an important role in the development of colon cancer. This study aims to evaluate the expression of CD55 in colon cancer and discover how it is regulated by transcriptional factors and miRNA.

Methods

The expression of CD55 was explored by TIMER2.0, UALCAN, and Human Protein Atlas (HPA) databases. TRANSFAC and Contra v3 were used to predict the potential binding sites of transcription factors in the CD55 promoter. TargetScan and starBase v2.0 were used to predict the potential binding ability of miRNAs to the 3′ untranslated region (3′UTR) of CD55. SurvivalMeth was used to explore the differentially methylated sites in the CD55 promoter. Western blotting was used to detect the expression of TFCP2 and CD55. Dual-luciferase reporter assay and chromatin immunoprecipitation (ChIP) assay were performed to determine the targeting relationship of TFCP2, NF-κB, or miR-27a-3p with CD55. CD55-related genes were explored by constructing a protein–protein interaction (PPI) network and performing pathway analysis by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG).

Results

CD55 was highly expressed in colon cancer tissues. The mRNA and protein expression levels of TFCP2 were reduced by si-TFCP2. NF-κB mRNA was obviously reduced by NF-κB inhibitor and increased by NF-κB activator. CD55 protein was also inhibited by miR-27a-3p. Dual-luciferase reporter assays showed that after knocking down TFCP2 or inhibiting NF-κB, the promoter activity of CD55 was decreased by 21% and 70%, respectively; after activating NF-κB, the promoter activity of CD55 increased by 2.3 times. As TFCP2 or NF-κB binding site was mutated, the transcriptional activity of CD55 was significantly decreased. ChIP assay showed that TFCP2 and NF-κB combined to the promoter of CD55. The luciferase activity of CD55 3′UTR decreased after being co-transfected with miR-27a-3p mimics and increased by miR-27a-3p antagomir. As the miR-27a-3p binding site was mutated, we did not find any significant effect of miR-27a-3p on reporter activity. PPI network assay revealed a set of CD55-related genes, which included CFP, CFB, C4A, and C4B. GO and KEGG analyses revealed that the target genes occur more frequently in immune-related pathways.

Conclusion

Our results indicated that CD55 is regulated by TFCP2, NF-κB, miR-27a-3p, and several immune-related genes, which in turn affects colon cancer.