bluuebunny/crossref_metadata_2025 huggingface dataset:
Electrochemical polymerization of azure blue II and its electrocatalytic activity toward NADH oxidation
Authors: Li Nian‐Bing , Duan Jian‐Ping , Chen Guo‐Nan
Published: September 2010

Poly (azure blue II) (PABII) thin film modified electrode was successfully assembled on the surface of a glassy carbon electrode by means of electrochemical polymerization, which was carried out with cyclic voltammetric sweeping in the potential range of ‐ 0.6 to + 1.3 V (vs. SCE) in Britton‐Robinson buffer solution (pH = 9.8) containing 1.25 ± 10–4 mol/L azure blue II. The effect of pH on the polymerization process of azure blue II and the electrochemical characteristics of the polymer‐modified electrodes were studied in detail. The experimental results indicated that the electropolymerization of azure blue II could take place in basic or neutral media. The cyclic voltammograms of poly (azure blue II) thin film modified electrode showed the presence of two couples of redox peaks. The film modified electrode exhibited potent and persistent electrocatalysis for oxidation of dihydronicotiamide adenine dinucleotide (NADH) in phosphate buffer media with a diminution of the overpotential of about 410 mV and an increase in peak current. The presence of some divalent cations in an electrolyte can greatly enhance the electrocatalytic current for oxidation of NADH. The electrocatalytic current increased linearly with NADH concentration from 1.0 ± 10–5 to 8.0 ± 10–3 mol/L in the presence of 4.0 ± 10–2 mol/L Mg2+ cation. The detection limit (3sb1/S) was 5.0 ± 10–6 mol/L, and the relative standard deviation of determination results was 4.2% for six successive determinations of 5.0 ± 10–4 mol/L NADH in the presence of Mg2+ cation.
Synthesis of cyclohexyl and isopropyl ketones from benzimidazole methiodide salt and grignard reagents
Authors: Yang Bing‐Qin , Qiang Ju‐Li , Bai Yin‐Juan , Lu Jun , Shi Zhen
Published: September 2010

The reaction of 2‐alkyl‐1,3‐dimethylbenzimidazolium salts with Grignard reagents gave addition products which were hydrolyzed to give ketones. A new method for the preparation of cyclohexyl ketones and isopropyl ketones is provided.
Conversion of 3‐carbethoxy‐4‐methyl coumarin derivatives into several new annelated coumarin derivatives
Authors: Mohammed Hadeer Bakeer
Published: September 2010

The reaction of ethyl esters of 4‐methyl‐2‐oxo‐2H‐1‐benzo (naphtho) pyran‐3‐carboxylic acids (1) with aromatic aldehydes in the presence of piperidine yielded 4‐styryl‐3‐carboxamidopiperidyl coumarin derivatives 4. The reaction of hydrazine hydrate with 1 gave acetophenone hydrozone derivatives 5 and acetophenone azine derivatives 6. The reaction of 1 with primary amines afforded compounds 7–9. And the treatment of la with Grignard reagents afforded 3‐aroyl‐4‐methyl coumarin derivatives 10.
Coupling reaction of carbonyl compounds mediated by gallium metal in aqueous media
Authors: Wang Zhi‐Yong , Yuan Shi‐Zhen , Zha Zheng‐Gen , Zhang Zu‐De
Published: September 2010

A simple and effective pinacol coupling of various aromatic aldehydes mediated by gallium in good yields has been carried out. The reaction is highly effective in water in the presence of KOH or HCl and was strongly affected by the steric environment surrounding the carbonyl group. Aliphatic aldehydes, ketones and aromatic ketones appear inert under the same reaction conditions.
Impact of genomics and in Silico related technologies in the drug discovery process
Authors: Léauté Jean‐Baptiste
Published: September 2010

In order to evaluate to what extent will genomics and in silico related technologies improve overall drug discovery process, we analyzed three studies comparing cost, time and attrition rate at each step of the drug discovery process, between standard pharmaceutical and genomics based approaches.
Contribution to structural elucidation: Behaviours of substructures partially defined from 2D NMR
Authors: Epouhe Celine , Fan Bo‐Tao , Yuan Shen‐Gang , Panaye A. , Doucet J. P.
Published: September 2010

Structural elucidation (automatic determination of the structure of a molecule from its spectra) is frequently hampered by combinatorial explosion when trying to assemble the identified substructures. We devised a new method which can avoid this pitfall by a systematic examination of allowed 13C chemical shifts ranges for all substructures chemically possible and combined with a progressive pruning thanks to neighbouring relationships appearing from 2D NMR. This method is explained by a detailed example.
Studies of nano‐sized Co3O4 as anode materials for lithium‐ion batteries
Authors: Huang Feng , Zhan Hui , Zhou Yun‐Hong
Published: September 2010

The structural evolution of the Co3O4 fine powders prepared by rheological phase reaction and pyrolysis method upon different temperature has been investigated using X‐ray diffraction (XRD) topography. The electrochemical performance of Co3O4 electrode materials for Li‐ion batteries is studied in the form of Li/Co3O4 cells. The reversible capacity as high as 930 mAh/g for the Co3O4 sample heat‐treated at 600 °C is achieved and sustained over 30 times charge‐discharge cycles at room temperature. The detailed information concerning the reaction mechanism of Co3O4 active material together with lithium ion is obtained through ex‐situ XRD topography, X‐ray photoelectron spectroscopy (XPS) analysis and cyclic voltammetry (CV) technique. And it is revealed that a “two‐step” reaction is involved in the charge and discharge of the Li/Co3O4 cells, in which Co3O4 active material is reversibly reduced into xCoO(3 ‐ x)CoO and then into metallic Co.
A novel fluorescent aminoacid designed for fluorometric detection of Cu(II)
Authors: Sun Xiang‐Ying , Wen Zhen‐Chang , Jiang Yun‐Bao
Published: September 2010

A fluorescent aminoacid was designed for selective and sensitive detection of Cu(II) in aqueous solution. The designing of this Cu(II) fluorescent chemosensing molecule, N ± (1‐naphthyl). aminoacetic acid (NAA), was based on the binding of Cu(II) to aminoacetic acid and the novel charge transfer photophysics of 1‐aminonaphthalenes. The fluorescence of NAA was found quenched by Cu (II) and several other metal ions of similar electronic structure such as Co(II), Ni(II) and Zn(II). The quenching was shown to occur via electron transfer within the metal‐NAA complex, which required an optimal combination of high binding affinity and favorable redox properties of the components in the metal‐NAA complex and hence afforded selective fluorometric detection of Cu(II). The calibration graph obeyed Stern‐Volmer theory and was shown for Cu(II) over the range of 0–2.75 ± 10–4 mol/L. The quenching constant of Cu(II) was measured as 8.0 ± 103 mol/L that was two orders of magnitude higher than those of Co(II), Ni(II) and Zn(II). The 3SD limit of detection for Cu(II) was 8.00 ± 10−6 mol/L with a coefficient of variation of 1.65%. Linear range for quantitative detection of Cu(II) was 2.67 ± 10−5‐2.75 ± 10−4 mol/L. The method was applied to synthetic sample measurements which gave recoveries of 105%‐112%.
Triterpenoid constituents of Huperzia miyoshiana
Authors: Tong Xiao‐Tian , Tan Chang‐Heng , Zhou Hui , Jiang Shan‐Hao , Ma Xiao‐Qiang , Zhu Da‐Yuan
Published: September 2010

Thirteen triterpenoids, including three new ones, miyoshianols A (1), B (2) and C (3), were isolated from Huperzia miyoshiana. The structures of these new compounds were established as 3‐O‐dihydroferuloyltohogenol (1), 16‐oxo‐3β, 21β‐dihydroxy‐serrat‐14‐en‐24‐ferulate (2) and 16‐oxo‐3α, 21β‐dihydroxyserrat‐14‐en‐24‐ferulate (3), respectively, on the basis of their spectroscopic analysis.
Calix[4] crowns with Methoxynaphthoylmethyl Pendant Groups
Authors: Jin Chuan‐Min , Lu Guo‐Yuan , Zhang Chao‐Zhi , Shi Zheng‐Wei , You Xiao‐Zeng
Published: September 2010

The novel calix [4] crowns with two pendant groups were prepared by the alkylation of calix [4] crowns with 6‐methoxy‐2‐bromoacetylnaphthalene. 1H NMR titration and picrate extraction experiments indicated that they exhibit higher complexing efficiency than their parent compounds and possess obvious selectivity for Na+ or K+, respectively, and that the cation is encapsulated inside the preorganized ionophoric cavity defined by carbonyl oxygens, the crown ether and the phenoxy oxygens. From UV and fluorescent spectra it is revealed that calix [4]‐crown‐4 3a with 6‐methoxy‐2‐naphthoylmethyl pedant groups exhibits remarkable cation‐induced photophysical effects and it could be utilized as a selective fluorescent sensor for Ca2+.
Substituent Effects on the Hydrogen Bonding between 4‐Substituted Phenols and HF, H2O, NH3
Authors: Cheng Yu‐Hui , Fu Yao , Liu Lei , Guo Qing‐Xiang
Published: September 2010

Density function theory UB3LYP/6–31 + g(d) calculations were performed to study the hydrogen bonds between para‐substituted phenols and HF, H2O, or NH3. It revealed that many properties of the non‐covalent complexes, such as the interaction energies, donor‐acceptor distances, bond lengths and vibration frequencies, showed well‐defined substituent effects. Therefore, from the substituent effects not only the mechanism of a certain non‐covalent interaction can be better understood, but also the interaction energies and structures of a certain non‐covalent complex, which otherwise might be very hard or resource‐consuming to estimate, can be easily predicted.
Study of Activation and Inhibition of Certain Metal Ions to Amylase Catalyzed Reaction by Microcalorimetry
Authors: Zhang Hong‐Lin , Yu Xhi‐Fang , Nie Yi , Liu Xiao‐Jing , Zhang Gang
Published: September 2010

With or without activation or inhibition of metal ion, the power‐time curves of amylase catalyzed reaction were determined by a 2277 thermal activity monitor (Sweden). The Michaelis constant (K), apparent Michaelis constant (Km), maximum velocity (vm) and apparent maximum velocity (vam) of amylase catalyzed reaction were obtained using thermokinetic theory and reduced extent method.

On the basis of data obtained, the following relationships between Km and concentration of metal ion (c) were established

for inhibitor of Ni2+

Km = 2.9648 × 10−3‐1.3912 × 10−4c

R =0.9998

for inhibitor of Co2+

Km = 1.0227 × 10−3 + 8.2676 × 10−6c

R = 0.9955

for activator of Ca2+

Km = 1.0630 × 10–7c2‐1.8311 × 10−6c + 9.3058 × 10−6

R = 0.9999

for activator of Li+

Km = 5.6300 × 10–8c2‐1.5329 × 10−6c + 1.2662 × 10−5

R = 0.9999

The Km‐c relationships show a strenuous inhibitory effect for Ni2+ and a strenuous active effect for Ca2+.
QSAR Studies on the COX‐2 Inhibition by 3,4‐Diarylcycloxazolones Based on MEDV Descriptor
Authors: Liu Shu‐Shen , Cui Shi‐Hai , Yin Da‐Qiang , Shi Yun‐Yu , Wang Lian‐Sheng
Published: September 2010

Selective inhibition of cyclooxygenase‐2 (COX‐2) might avoid the side effects of current available nonsteroidal antiinflammatory drugs while retaining their therapeutic efficacy. A novel variable selection and modeling method based on prediction is developed to construct the quantitative structure‐activity relationships (QSAR) between the molecular electronegativity distance vector (MEDV) based on 13 atomic types and the biological activities of a set of selective cyclooxygenase‐2 inhibitory molecules, 3,4‐diarylcycloxazolones (DAA) plus indomethacin, naproxen, and celecoxib. Using multiple linear regression, a 5‐variable linear model is developed with the calibrated correlation coefficient of 0.9271 and root mean square error of 0.17 in modeling stage and the validated correlation coefficient of 0.9030 and root mean square error of 0.20 in leave‐one‐out validation step, respectively. To further test the predictive ability of the model, 20 DAA compounds are picked up to construct a training set which is used to build a QSAR model and then the model is employed to predict the biological activities of the balance compounds. The predicted correlation coefficient and root mean square error are 0.9332 and 0.19, respectively.
Synthesis and Liquid Crystal Behaviors of 2,4‐Dioxo‐3‐pentyl 4‐decyloxy Cinnamate Rhodium(I) Complexes
Authors: Han Jie , Zhang Liang‐Fu , Wan Wen
Published: September 2010

The title complexes have been synthesized by the reaction of [RhCl(CO)2]2 or [RhCl(COD)]2 (COD = 1,5‐cyclooctadiene) with the organic ligand 2,4‐dioxo‐3‐pentyl 4‐decyloxy cinnamate 2. The complex 3 based on dicarbonylrhodium(I) shows nematic phase, while the complex 4 containing rhodium(I) bound to a COD ligand is a nonmesogen. The relationship between molecular structures and liquid crystal behavior has also been discussed by means of computer‐aided molecular modeling.
Morphology Control over the Organic Nanoparticles of 1, 3‐Diphenyl‐5‐(9‐anthryl)‐2‐pyrazoline
Authors: Xi Lu , Xiao De‐Bao , Yang Wen‐Sheng , Yao Jian‐Nian , Zhang Bao‐Wen
Published: September 2010

The organic nanoparticles of a blue‐light‐emitting molecule, 1, 3‐diphenyl‐5‐(9‐anthryl)‐2‐pyrazoline, were prepared by reprecipitation method using acetonitrile as the solvent for the molecular precursor. Three morphologies, spherical, doughnut‐shaped and cubic, could be observed on the silicon substrate for the nanoparticles by the volume‐controlled addition of acetonitrile. The evolution of particle morphology as a function of acetonitrile addition was attributed to the variation of the growth habits of the particles in the different environment. The nanoparticles exhibit the novel photoluminescence spectra as compared to those of monomer and the bulk crystals.
Total Synthesis of (‐)‐Kaerophyllin, (‐)‐Hinokinin and (±)‐Isohinokinin
Authors: Xia Ya‐Mu , Liang Qi‐Ren , Wang Xiao‐Long , Cao Xiao‐Ping , Pan Xin‐Fu
Published: September 2010

A convenient and rapid approach for the syntheses of (‐)‐kaerophyllin (1), (‐)‐hinokinin (2) and (±)‐isohinokinin (3) was described. The key steps were involved in condensation of aromatic aldehyde and alkylation of the resulting ester to give the complete skeleton of dibenzylbutyrolactone‐lignan. Hydrolysis, followed by resolution with quinine, reduction and when appropriate, oxidation gave the title compound. The asymmetric total synthesis of the kaerophyllin (1) was reported for the first time.
Molecular Dynamics Studies of the Kinetics of Phase Changes in Clusters IV: Crystal Nucleation from Molten (NaCl)256 and (NaCl)500 Clusters
Authors: Li Xiao‐Hua , Huang Jin‐Fan
Published: September 2010

Molecular dynamics computer simulation based on the Born‐Mayer‐Huggins potential function has been carried out to study the effects of cluster size and temperature on the nucleation rate of sodium chloride clusters in the temperature range of 580 K to 630 K. Clusters with 256 and 500 NaCI molecules have been studied and the results have been compared with those obtained from 108 molecule clusters. The melting point (MP) of the dusters were observed to increase with the size of the clusters and can be well described by a linear equation MP = 1107(37)‐1229(23)N−1/3(N is the number of molecules in the cluster). The nucleation rate was found to decrease with increasing the cluster size or temperature. Various nucleation theories have been used to interpret the nucleation rates obtained from this molecular dynamics simulation. It is possible to use a constant diffuse interface thickness to interpret the nucleation rate from the diffuse interface theory in the temperature range of this study. However, the interfacial free energy estimated from classical nucleation theory and diffuse interface theory increases too fast with increasing the temperature while that from Gran‐Gunton theory does not change with changing temperatures. The sizes of critical nuclei estimated from all the theories are smaller than those estimated from our simulations.
A New Formulation of the Lindemann Mechanism of Unimolecular Reactions
Authors: Qian Ren‐Yuan
Published: September 2010

A new formulation of the Lindemann mechanism of unimolecular reactions in gaseous phase is presented, without the use of steady state hypothesis. It is hereby shown that the nature of applicability of steady state hypothesis in the regime of high reactant gas pressure is different from that in the regime of low gas pressure. In the former case it is an equilibrium approximation, while in the latter case it is a highly reactive intermediate approximation in no connection with a steady state. Furthermore for the latter case it is shown that in the classical formulation of Lindemann mechanism the use of steady state hypothesis is an ad hoc assumption. A highly reactive intermediate in the sense that its concentration is very small during the whole course of reaction is a necessary condition for the applicability of very reactive intermediate approximation. When the two distinctive nature of the applicability of steady state hypothesis is mixed‐up, wrong or useless conclusion may be arrived at. The only possible case of realizing a true steady state in a complex reaction is pointed out.
DAR Assisted Layer‐by‐Layer Assembly of Aromatic Compounds
Authors: Jiang Si‐Guang , Chen Xiao‐Dong , Zhang Li , Liu Ming‐Hua
Published: September 2010

A facile DAR (diphenylamine‐4‐diazonium‐formaldehyde resin) assisted layer‐by‐layer (LbL) assembly of ultrathin organic film of aromatic compounds has been investigated. The multilayer of pyrene or anthracene was fabricated through simple dipping of the glass slide into the mixed solution of DAR with the target compounds. In this method, DAR acted as an assistant compound to help the assembling of the aromatic compounds. Such a convenient deposition method not only reserves the advantages of the traditional LbL technique but also simplifies the technique and extends the effectiveness of LbL technique to small molecules without any charge.
On the Importance of CP‐corrected Gradient Optimization in the Study of Hydrogen Bonded Systems
Authors: Wang Wei‐Zhou , Pu Xue‐Mei , Zheng Wen‐Xu , Wong Ning‐Bew , Tian An‐Min
Published: September 2010

Geometries, harmonic vibrational frequencies and interaction energies of the water‐hydrogen sulfide dimer, hydrogen fluoride dimer and glycine zwitterion‐water dimer were determined by the counterpoise‐corrected (CP‐corrected) gradient optimization that explicitly corrects for the basis set superposition error (BSSE) and CP‐uncorrected (normal) gradient optimization respectively at the B3LYP and MP2 levels of theory, employing the popular Pople's standard 6–31G(d), 6–31G(d, p) and 6–311 + + G(d, p) basis sets in order to assess the importance of CP‐corrected gradient optimization in the study of hydrogen bonded systems. The normal optimization of these three H‐bonded systems obtained using these popular basis sets all yielded erratic results, whereas use of CP‐corrected gradient optimization led to consistent results with those from larger basis sets. So this CP receipt becomes useful and necessary to correctly describe large systems, where the use of small basis sets may be necessary.
3D‐QSAR Study on Apicidin Inhibit Histone Deacetylase
Authors: Chen Hai‐Feng , Kang Jiu‐Hong , Li Qiang , Zeng Bao‐Shan , Yao Xiao‐Jun , Fan Bo‐Tao , Yuan Shen‐Gang , Panay A. , Doucet J. P.
Published: September 2010

For Histone Deacetylase (HDAC) Inhibitor, four 3D‐QSAR models for four types of different activities, were constructed. The cross‐validated q2 value of CoMFA Model 1 is 0.624 and the noncross‐validated r2 value is 0.939. The cross‐validated q2 value of Model 2 for training set is 0.652 and the noncross‐validated r2 value is 0.963. The cross‐validated q1 value for Model 3 is 0.713, with noncross‐validated r2 value 0.947. The cross‐validated q2 value for Model 4 is 0.566 with noncross‐validated r2 value 0.959. Their predicted abilities were validated by different test sets which did not include in training set. Then the relationship between substituents and activities was analyzed by using these models and the main influence elements in different positions (positions 8 and 14) were found. The polar donor electron group of position 8 could increase the activity of inhibition of HDAC, because it could form chelation with the catalytic Zn. Suitable bulk and positive groups at position 14 are favorable to anti‐HDAC activity. These models could well interpret the relationship between inhibition activity and apicidin structure affording us important information for structure‐based drug design.
Synthesis, Structure and Biological Activities of S‐[a‐(4‐Methoxyphenylcarbonyl)‐2‐(1, 2, 4‐triazole‐1‐yl)] ethyl‐N, N‐dimethyldithiocarbamate
Authors: Xu Liang‐Zhong , Jian Fang‐Fang , Gao Hong‐Rong , Zhu Chong‐Yi
Published: September 2010

The title compound was prepared by reaction of N, N‐dimethyldithiocarbamate sodium with l‐bromo‐l‐(4‐methoxyphenylcarbonyl)‐2‐(1, 2, 4‐triazole‐l‐yl) ethane. Its crystal structure has been determined by X‐ray diffraction analysis.

The crystal belongs to triclinic with space group Pī, a = 0.7339(2) nm, b = 1.1032(2) nm, c = 1.1203(2) nm, a = 90.27(3)°, β = 102.03(3)°, γ = 104.91(3)°, Z=2, V = 0.8556(3) nm3, Dc = 1.360 g/cm3, μ =0.325 mm−1, F(000)=368, final R1 =0.0475. The planes of 4‐methoxybenzyl group and triazole ring are nearly perpendicular to each other. The dihedral angle is 83.97°. There is an obvious π‐π stacking interaction between the molecules in the crystal lattice. The results of biological test show that the title compound has fungicidal and plant growth regulating activities.
A Novel Glucose Biosensor Based on Palladium Nanoparticles and Its Application in Detection of Glucose Level in Urine
Authors: Zhang Fen‐Fen , Wan Qiao , Li Chen‐Xin , Zhu Zi‐Qiang , Xian Yue‐Zhong , Jin Li‐Tong , Yamamoto Katsunobu
Published: September 2010

A novel glucose biosensor was constructed by electrodeposition of highly dispersed palladium (Pd) nanoparticles on a glassy carbon electrode (GCE). Atomic force microscopy (AFM) was applied to characterize its surface morphology. Electrodeposited Pd nanoparticles exhibited efficiently electrocatalytic oxidation for hydrogen peroxide (H2O2) with relatively high sensitivity and stability, which was studied by CV technique and Raman spectroscopy, respectively. The GC/PdVGOD/Nafion system allowed a low working potential of +0.3 V (vs. SCE). Its signal current was linearly related to the glucose concentration in the range of 1.0 × 10–6‐1.2 × 10–4mol × L−1 with a detection limit of 5.0 × 10–7 mol · L−1. The sensor required no special pretreatment to suppress interference from urate and L‐ascorbate. It was successfully used in detection of glucose level in human urine with high stability, sensitivity and anti‐poisoning ability.
Synthesis of l‐Benzoyl‐3‐aryl‐4‐hydroxy‐4‐phenylimidazolidin‐2‐thiones
Authors: Zeng Run‐Sheng , Zou Jian‐Ping , Mu Xue‐Jun , Shen Qi
Published: September 2010

1‐Benzoyl‐3‐aryl‐4‐hydroxy‐4‐phenylimidazolidin‐2‐thiones can be synthesized readily from the cyclization of l‐benzoyl‐3‐arylthioureas with bromine‐acetophenone in the presence of excess triethylamine.
Study on the Phase Equilibrium of the Ternary System Ethanol‐Cesium Carbonate‐Water at 10, 30 and 50 °C
Authors: Zhai Quan‐Guo , Hu Man‐Cheng , Liu Zhi‐Hong , Xia Shu‐Ping
Published: September 2010

The phase diagrams of the CH3CH2OH‐Cs2CO3‐H2O system were determined at 10, 30 and 50 C, respectively. It was found that the effect of the temperature on the phase equilibrium was insignificant within the investigated range. The binodal curves were given using a five‐parameter equation and the tie lines were fitted using the Oth‐mer‐Tobias and Bancroft correlations. Correlation coefficients (R) for all equations exceeded 0.99. Samples of the solid phase at 30 °C were analyzed by TGA showing that it consisted of Cs2CO3. 3.5H2O. The refractive index of the systems was also determined.
Study on Tonghaosu and Its Analogs: Isolation, Structure Identification and Synthesis of Antifeedant B‐ring‐homo‐tonghaosu
Authors: Chen Li , Yin Biao‐Lin , Xu Han‐Hong , Chiu Ming‐Hua , Wu Yu‐Lin
Published: September 2010

The methanolic extract from a Chinese endemic Chrysanthemum plant, Dendranthema indicum var. aromaticum, was found to show high antifeeding activity against Pieris brassicae L., and by bioassay‐guided separation, the active component, B‐ring‐homo‐tonghaosu, 2–(2′.4′‐hexadiynylidene)‐1, 6‐dioxaspiro‐[4, 5]dec‐3‐ene (2) was isolated. Its structure was elucidated by comparing its spectroscopic data with those of 2 reported in the literatures. Furthermore new convenient total synthesis methods of B‐ring‐homo‐tonghaosu were also developed to confirm its structure and make its further application in crop protection available. In addition, extensive comparison of spectroscopic data showed that the structure of compound 21 reported in literature should be revised to 2.
New Myrsinol Diterpenes from Euphorbia prolifera
Authors: Zhang Wan‐Jin , Chen Dao‐Feng , Hou Ai‐Jun
Published: September 2010

Four new myrsinol diterpenes, euphorprolitherins A‐D (1–3, 5), were isolated from the ethanolic extract of the mot of Euphorbia prolifera, along with a known one. SPr5 (4). The structures were elucidated on the basis of their spectroscopic evidences. The structure and relative stereochemistry of 1 were confirmed by X‐ray crystallography.
Synthesis and Luminescent Properties of an Acetylide‐Bridged Dinuclear Platinum(II) Terpyridyl Complex
Authors: Wang You‐Wei , Yang Qing‐Zheng , Wu Li‐Zhu , Zhang Li‐Ping , Tung Chen‐Ho
Published: September 2010

An acetylide‐bridged dinuclear platinum(II) terpyridyl complex, [Pt(4‐p‐tolyl‐terpy)‐i‐phenyl‐=‐(4′‐p‐tolyl‐terpy)Pt](C104)2 (1). has been successfully synthesized and its photophysical properties are reported.
Preparation and Charactrization of Fibrous Crystals of Boron‐containing MTW‐type Zeolite
Authors: Song Mao‐Ying , Zhou Wei‐Zheng , Long Ying‐Cai
Published: September 2010

Fibrous crystals of boron‐containing MTW‐type zeolite have been hydrothermally synthesized in B2O3‐SiO2‐HF‐H2O gel system at 170 °C in 20 to 28 d by using 1, 4‐diazabicyclo[2.2, 2]octane (DABCO) and methylamine as the co‐template, and characterized with XRD, SEM, TEM, HRTEM and SAED. The results of characterizations show that B atoms are incorporated into the zeolite framework as tetrahedron of B(OSi)4. The fibrous single crystals of 5–50 μm in length and 100–500 nm in width inter‐grow along the c‐axis of the zeolite, and the one dimension 12 oxygen ring channels are perpendicular to the fibber axis.
Thermochemical Properties and Non‐isothermal Decomposition Reaction Kinetics of N‐Guanylurea Dinitramide (GUDN)
Authors: Zhao Feng‐Qi , Chen Pei , Yuan Hong‐An , Gao Sheog‐Li , Hu Rong‐Zu , Shi Qi‐Zhen
Published: September 2010

The constant‐volume combustion energy, ΔcU (GUDN, s, 298.15 K), enthalpy of solution in acetic ether, Δ Hθm and kinetic behavior of the exothermic decomposition reaction of the title compound (GUDN) are determined by a precise rotating bomb calorimeter, a Calvet microcalorimeter and DSC, respectively. Its standard enthalpy of combustion, Δ, Hθm: (GUDN, s, 298.15 K), standard enthalpy of formation, Δ, Hθm (GUDN, s, 298.15 K) and kinetic parameters of the exothermic main decomposition reaction in a temperature‐programmed mode [the apparent activation energy ( Ea ) and pre‐exponential factor (A)] are calculated. The values of ΔcU (GUDN, s, 298.15 K), Δ Hθm (GUDN, s, 298.15 K), Δ, Hθm (GUDN, s, 298.15 K) and Δsol Hθm of GUDN are (‐7068.64×2.37) I‐g (‐1467.66×0.50) kJ · mol−1, (‐319.76×0.58) kJ · mol−1 and (165.737×0.013) kJ·mol−1, respectively. The kinetic model function in integral form and the value of Ea and A of the exothermic main decomposition reaction of GUDN are 220.20 kl · mol−1 and 1021.18 s−1, respectively. The critical temperature of thermal explosion of GUDN is 217.6 °C.
Oxygen Atom Adsorption and Diffusion on Pd Low‐index Surfaces and (311) Stepped Surface
Authors: Wang Ze‐Xin , Jia Xiang‐Feng , Tian Feng‐Hui , Chen Shou‐Gang
Published: September 2010

The 5‐parameter Morse potential (5‐MP for short) of the interaction system between an oxygen atom and palladium surface clusters was constructed. The adsorption and diffusion of an oxygen atom on low index surfaces Pd (100). Pd (111). Pd (110) and Pd (311) stepped surface were investigated in detail with 5‐MP. It is found that fcc and hcp sites on the (111) surface and (111) microfacets are equivalent. The calculation results show that O atom adsorbs in the three‐fold hollow site, and the long‐bridge site is a stable site both in regular Pd (110) surface and in the (1×2) missing‐row reconstruction structure. Moreover, in the study of O‐Pd (311) surface system, We conclude that there are two stable adsorption states (four‐fold site: H4, three‐fold site: Hn) on O‐Pd (311) surface and the three‐fold site (Hf) is the metastable adsorption. At low coverage oxygen atom favors the four‐fold hollow site (H4).
Baker's Yeast Mediated Reduction of Optically Active Diketone
Authors: Zheng Guo‐Jun , Gao Xiao‐Lei , Chen Jin‐Chun , Li Yu‐Lin
Published: September 2010

Baker's yeast mediated reduction of optically active diketone is described. The two keto pups are efficiently differentiated and the ee value of the recovered material is considerably raised. It affords highly optically active key intermediates efficiently for the synthesis of natural polyhydroxylated agarofuran products.
Solid Phase Synthesis of 2‐Substituted1, 3‐Oxazin‐6‐ones Using Resin‐bound Cyclic Malonic Acid Ester
Authors: Liu Zhan‐Xiang , Ruan Xiu‐Xiu , Huang Xian
Published: September 2010

A facile solid phase. synthesis of 2‐substituted1,3‐oxazin‐6‐ones using polymer‐supported Meldrum's acid has been reported. Reaction of the resin‐bound cyclic malonic acid ester with triethyl orthoformate and subsequent double substitution with amide, afforded the corresponding polymer‐supported acylaminomethylene cyclic malonic acid ester, which upon thermal treatment led to1, 3‐oxazin‐6‐ones in good yields and with high purity.
A Facile One‐pot Synthesis of2,5‐Disubstituted Oxazoles Using Poly[styrene(iodosodiacetate)]
Authors: Huang Xian , Chen Jiang‐Min
Published: September 2010

2.5‐Disubstituted oxazoles were prepared conveniently by treatment of aromatic α‐methyl ketones and nitriles with poly[styrene(iodosodiacetate)] in a one‐pot manner.
Transport Properties of Fluids in Micropores by Molecular Dynamics Simulation
Authors: Liu Ying‐Chun , Wang Qi , Lü Ling‐Hong
Published: September 2010

The transport properties of fluid argon in rnicropores. i.e. diffusivity and viscosity. were studied by molecular dynamics simulations. The effects of pore width, temperature and density on diffusivity and viscosity were analyzed in micropores with pore widths from 0.8 to 4.0 run. The results show that the diffusivity in micropores is much lower than the bulk diffusivity, and it decreases as the pore width decreases; but the viscosity in micropores is significantly larger than the bulk one, and it increases sharply in narrow micropores. The diffusivity in channel parallel direction is obviously larger than that in channel perpendicular direction. The temperature and density are important factors that obviously affect diffusivity and viscosity in micropores.
Theoretical Studies on the Third‐order Nonlinear Optical Properties and Two‐photon Absorption of Stilbene Derivatives
Authors: Ai‐Min Ren. , Feng Ji‐Kange , Liu Xiao‐Juan
Published: September 2010

Different types of stilbene derivatives (D‐‐D, A‐‐A, D‐‐A) were investigated with AM1, and specially, equilibrium geometries of symmetrical stilbene derivatives (D‐‐D) were studied using of PM3. With the same method INDO/CI, the UV‐vis spectra were explored and the position and strength of the two‐photon absorption were predicated by Sum‐Over‐States expression. The relationships of the structures, spectra and nonlinear optical properties have been examined. The influence of various substituents on two photon absorption cross‐sections was discussed micromechanically.
Kinetic Studies on Na+/K+‐ATPase by Using Thermokinetic Method
Authors: Li Xia , Li Jie , Wang Zhi‐Yong , Xie Xiu‐Yin , Yang Xi , Wang Cun‐Xin
Published: September 2010

Na+/K−‐ATPasc (EC 3.6.1.3) is an important membrane‐bound enzyme. By using microcalorimetry, the thermokinetic method was developed to kinetic studies on Na+/K+‐ATPase for the first time. Compared with other ones, the method provided accurate measurements of not only thermodynamic data but also the kinetic data. At 310.15 K and pH=7.4, the molar reaction enthalpy ΔrHm, was measured as (‐40.408k 1.9) kJ mol−1. The Michaelis constant Km was determined to be (0.479 0.020) 10−‐3 mol L−1 and consistent with literature figure which is about 0.5 10−‐3 moi L−1. The maximum velocity Vmax obtained was (0.6810.026) μmol Pi min−1 mg protein−1. All of the data have good repeatability and self‐consistency. The reliability of thermokinetic method was verified by the experimental results and further confirmed by colorimetric studies. Moreover. the effect of enzyme pre‐dilution on its activities was also investigated.
Hydrothermal Synthesis and Crystal Structure of [Mn(phen)‐(m‐phth)]n (phen=1, 10‐phenanthroline, m‐phth=isophthalate)
Authors: Bi Wen‐Hua , Sun Dao‐Feng , Cao Rang , Li Xing , Shi Qian
Published: September 2010

Hydrothermal reaction of1,2,4‐benzenetricarboxylic acid, and MnCl24H2O with1,10‐phenanthroline gave the title complex, [Mn(phen)(m‐phth)]n, which crystallizes in the monoclinic system, space group P2/c with a= 0.83582(3) nm, b=1.04246(4) nm, c=1.87777(7) nm, β= 100.314(2), V=1.60%8(10) nm3, Z=4, and the final R =0.0576, wR =0.1294. In the complex, each Mn(II) ion is coordinated to four O atoms of carboxylate groups and two N atoms of phen. Isophthalate was generated in situ through the loss of one carboxylate group of1,2,4‐benzenetricarboxylic acid under the hydrothermal condition. Each isophthalate is bound to three manganese atoms through O atom of carboxylate in bridging and chelating fashion, affording manganese(II) pairs that are linked by the isophthalate group to yield a zig‐zag chain along the c‐axis.
Flow‐Injection Electrochemiluminescence Detecting Rifampicin Based on Its Sensitizing Effect
Authors: Ma‐Yan Hong , Zheng Xing‐Wang , Zhang Zhu‐Jun
Published: September 2010

A new flow‐injection electrogenerated chemieluminescence (ECL) method for the determination of rifampicin was developed. The method is based on the sensitizing effect of rifampicin on the weak ECL signal of electrochemical oxidation of luminol on the surface of the platinum flake electrode in the medium of 0.02 mol L−1 Na2B4O7. This ECL response is in the range of 1.0 10–4.0 10−‐1 mol L−1 rifampicin with a detection limit of 8.0 10−‐9 mol L−1. The relative standard deviation is 1.8%. This method has been applied successfully to the determination of rifampicin in capsule. ocustilla and urine samples.
Design, Synthesis and Pregnancy‐Terminating Activity of 2‐Aryl Imidazo[2, 1 ‐a]isoquinolines
Authors: Shang Zhi‐Cai , Hu Gui‐Xiang , Wu Tian‐Xing , Fang Yui‐Ying , Yu Qing‐Sen
Published: September 2010

In order to clarify the structural requirement of pregnancy‐terminating drugs, the quantitative structure‐activity relationship (QSAR) of 2‐aryl imidazo[2, 1‐a]isoquinolines was studied on the basis of quantum mechanical calculation and multiple regression analysis. A Good correlation equation was obtained (r2=0.925, q2=0.871). Some new compounds were designed according to the equation. Two of them, compounds 21 and 22, were synthesized and evaluated in NIH mice. The results showed that the difference of activity between 21 (median effective dose ED%=0.943 mg/kg/day) and 22 (ED50= 1.099 mg/kg/day) was small and both of them were potent. It is also agreed with the computational results. Compared with L14105 which is the most potent pregnancy‐terminating agent, these two compounds possess high activity. The evaluation of the anti‐implanting activity showed that they were 100% effective at tested dosage 50.0, 25.0, 12.5 mg/kg/day 3 days in oral administration, which proved the both of them had anti‐implanting activity and low first‐pass effects.
Microencapsulation of n‐Eicosane as Energy Storage Material
Authors: Lan Xiao‐Zheng , Tan Zhi‐Cheng , Zou Guang‐Long , Sun Li‐Xian , Zhang Tao
Published: September 2010

For heat energy storage application, polyurea microcapsules containing phase change material, n‐eicosane, were synthesized by using interfacial polymerization method with toluene‐2,4‐diisocyanate (TDI) and diethylenetriamine (DETA) as monomers in an emulsion system. Poly(ethylene glycol)octyl‐phenyl ether (OP), a nonionic surfactant, was the emulsifier for the system. The experimental result indicates that TDI was reacted with DETA in a mass ratio of 3 to 1. FT‐IR spectra confirm the formation of wall material, polyurea, from the two monomers, TDI and DETA. Encapsulation efficiency of n‐eicosane is about 75%. Microcapsule of n‐eicosane melts at a temperature close to that of n‐eicosane, while its stored heat energy varies with core material n‐eicosane when wall material fixed. Thermo‐gravimetric analysis shows that core material n‐eicosane, micro‐n‐eicosane and wall material polyurea can withstand temperatures up to 130, 170 and 250 °C, respectively.
SnCl2/Cu‐mediated carbonyl allylation reaction in water: Scope, selectivity and mechanism
Authors: Tan Xiang‐Hui , Hou Yong‐Quan , Liu Lei , Guo Qing‐Xiang
Published: September 2010

Copper was found to be able to promote the SnCl2‐mediated carbonyl allylation reactions in water, giving the corresponding homoallylic alcohol products in very high yields. Detailed studies showed that the reaction could be applied to a variety of carbonyl compounds including those with hydroxyl, amino and nitro groups. It was also found that this reaction showed good regioselectivities for some substrates. Furthermore, carefully controled experiments and in situ NMR measurements provided important insights into the mechanism of the newly developed reaction.
Ramberg‐Bäcklund Rearrangement Approaches to the Synthesis of Natural Bibenzyls
Authors: Wang Xiao‐Long , Liu Dong , Xia Ya‐Mu , Cao Xiao‐Ping , Pan Xin‐Fu
Published: September 2010

The Ramberg‐Bäcklund rearrangement has been successfully utilized to convert readily available α‐ and α'‐hydrogen bearing substituted dibenzyl sulfones into corresponding stilbene derivatives, which is conveniently followed by hydrogenation in the presence of 10% Pd/C to afford the natural bibenzyls.
Synthesis of (ρ‐formylphenyl)azo calix[4]arenes
Authors: Bal Zhu , Yu Lei , Lu Guo‐Yuan , Guo Xun
Published: September 2010

Five novel azo calix[4]arenes were reported. The p‐aminobenzaldehyde was diazotized with sodium nitrite in aqueous hydrochloride solution. Mono‐, bis‐, his‐ and tetrakis(p‐formylphenyl)azo calix[4]arenes (including proximal and distal isomers) were obtained respectively by diazo‐coupling in different molar ratio to calix[4]arene (1) under pH=7.5‐8.5 at 0‐5 C. All (p‐formylphenyl)azo calix[4]arenes were characterized by 'H NMR, 13C NMR, IR, MS (ESIMS) spectroscopies and elemental analysis.
A two‐dimensional lanthanide coordination framework with a new amide‐type tripodal ligand,2,2′,2″‐nitrilotris{[(2′‐benzylaminoformyl)phenoxy]ethyl}amine
Authors: Fan Jun , Zhang Wei‐Guang , Tan Min‐Yu , Tang Yu , Liu Wei‐Sheng , Tang Ning , Yu Kai‐Bei
Published: September 2010

The La(III) complex with a new amide‐type tripodal ligand,2,2′.2″‐nitrilotris{[(2′‐benzylaminofomyl)‐phenoxylethyl)amine (L), was synthesized and characterized by X‐ray crystallographic analysis. Crystal data: C48H55.50LaN7O18.75, Mr=1169.40, monoclinic. space group, P21/n, a= 1.0644(3) nm. b=2.3889(5) nm, c= 2.1917(5) nm, β=90.65°, V=5.573(2) nm3, Z=1, Dc=1.394 g°cm−3, R1=0.0487, wR [1>2s̀(I)]=0.1266. The results reveal that each La(III) ion binds to 9 oxygen atoms, three of which belong to carbonyl groups from three tripodal ligands and six to three bidentate nitrate groups and a two‐dimensional sheet of 4.82 networks is assembled by metal‐ligand coordination interaction. L, a heptadentate compound, merely acts as a tridentate bridging spacer due to its steric hindrance and links the La(III) ions as three‐connected nodes.
Microcalorimetric investigation on the kinetics of the oxidation of ascorbic acid with hydrogen peroxide
Authors: Meng Xiang‐Guang , Kou Xing‐Ming , Xie Jia‐Qing , Du Juan , Zeng Xian‐Cheng
Published: September 2010

Based on the thermokinetic theory, a novel thermokinetic research method‐self‐function regression method, which could be used to determine the kinetic parameters kl. k2 and k‐1 of the complex reaction: B + \documentclass{article}\usepackage{chemarr}\pagestyle{empty}\begin{document}${\rm C} \xrightleftharpoons[{k_{- 1}}]{{{\rm k}_{\rm 1}}} {\rm I} \xrightarrow[]{{\rm k}_{\rm 2}} {\rm P},$\end{document}, was proposed in this paper. The kinetics of the reaction of ascorbic acid (H2A) with hydrogen peroxide was investigated at pH=5‐7 and T=298.15 K, and the kinetic parameters k1 k2 and k‐1 were obtained with this method. The kinetics of the reaction was investigated in detail. The kinetic parameters of the oxidation of ascorbic monoanion (HA−) and ascorbic dianion (A2‐) with hydrogen peroxide were 3.33×10−3 s−1 and 25.48 s−1 at 298.15 K, respectively. The molar enthalpy change for the reaction was ‐554.64 kJ · mol−1 in phosphate buffer solution at pH 7 and at 298. 15 K.
Effects of temperature raising speed on the growth of BN crystals in hydrothermal solutions
Authors: Yu Mei‐Yan , Li Kai , Cui De‐Liang , Dong Shou‐Yi , Wang Qi‐Long , Jiang Min‐Hua
Published: September 2010

Cubic boron nitride (cBN) and orthorhombic boron nitride (oBN) crystals have been prepared in hydrothermal solutions by reacting H3BO3+NaN3+P and H3BO3+NaN3+N2H4 respectively. The experimental results indicated that, if the temperature was increased rapidly, both the yield and perfectness of BN crystals became poor. On the contrast, the yield and perfecmess of BN crystals can be improved very much by slowly increasing the temperature of the reaction mixture. The results of X‐ray powder diffraction (XRD), Fourier transform infrared spectrum (FTIR) and high resolution transmission electron microscopy (HRTEM) proved that the samples were composed of oBN and cBN.
A,C‐bridged calix[6]arene: Relationship between the length of bridge and conformation
Authors: Gong Shu‐Ling , Chen Yi‐Kun , Li Jun , Duan He‐Ping , Chen Yuan‐Yin
Published: September 2010

Three new A,C‐diamide bridged p‐tert‐butylcalix[6]arenes were synthesized from p‐tert‐butylcalix[6]arenes by bridging ClCH2CONH(CH2)nNHCOCH2Cl (n=3, 4, 6) in acetonitrile using K2CO3 as a base in 17%‐25% yields. It was found that the bridged calix[6]arenes with shorter bridges (n=2, 3, 4 in N', N'‐bischloroacetodiamines) adopt cone conformation, but the last one (n=6) adopts alternate conformation, i.e., accompanying the lengthening of bridge, the conformation of A,C‐bridged calix[6]arenes changes from cone to alternate.
Immobilization of HX: [Hmim]X as halogenating agent, recyclable catalyst and medium for conversion of alcohols to alkyl halides
Authors: Wu Hai‐Hong , Sun Jing , Yang Fan , Tang Jie , He Ming‐Yuan
Published: September 2010

HX is immobilized by reaction of halogen acid with methylimidazole, and the formed ionic liquid [Hmim]X was used as halogenating agent, catalyst as well as medium for conversion of alcohols to alkyl halides. Excellent yields were obtained. The halides produced could be easily separated from the reaction mixture via simple decantation, and the ionic liquid [Hmim]X could be regenerated conveniently by adding equivalent of halogen acids followed by removal of water.
Dialdehyde in heterocyclic synthesis: Synthesis of compounds containing two 4H‐benzopyran building blocks under microwave irradiation
Authors: Feng You‐Jiana , Miao Chun‐Bao , Gao Yuan , Tu Shu‐Jiang , Fang Fang , Shi Da‐Qing
Published: September 2010

The syntheses of 4H‐benzopyran derivatives were investigated using dialdehyde as a key starting material. The reaction proceeds under microwave irradiation in good yield (87%‐95%) with short reaction time (5‐8 min), therefore providing a rapid and efficient method of synthesizing a variety of compounds containing two 4H‐benzopyran units.
Studies on natural CXN zeolite: Modification, framework De‐alumination and ion‐exchange
Authors: Long Ying‐Cai , Xia Xiao‐Hui , Yang Bo , Zhang Ling‐Mei , Zhou Wei‐Zheng , Cao Zhi‐Long , Li Cai‐Yun
Published: September 2010

A natural CXN zeolite (stilbite, type code‐STI) discovered in China was modified with NH: exchange by using ammonium salt and calcinations (procedure I), or with NH: exchange followed by treatment with acid (procedure rr). The coordination state of Si and Al atoms in the framework. the property of ion exchange, and the adsorption of the H‐STI zeolite samples prepared by different modification procedure were investigated with XRD, EDX, 29Si and 27Al MAS NMR, Ag+ ion exchange and N2 adsorption. The results of the investigations indicate that different procedure of the modification made variety on the distribution of the framework Si atoms and A1 atoms, the content of non‐framework aluminum. and the blocking channels and the shielding effect to the positions of the exchangeable cations. The H‐STI zeolite prepared by the procedure II possesses high ion exchange capacity, open and perfect pore system, and hgh thermal stability.
Synthesis, structure and biological activities of novel triazole compounds containing N,N‐dialkyldithiocarbamate moiety
Authors: Xu Liang‐Zhong , Jian Fang‐Fang , Shi Jian‐Gang , Sun Ping‐Ping , Jiao Kui
Published: September 2010

The two compounds, 2, 2‐dimethy1‐4‐S‐(N,N‐ dimethyldithiocarbamato)‐5‐(1, 2, 4‐triazol‐1‐yl)‐propione (1) and 2, 2‐dimethyl‐4‐S‐(N,N,‐diethyldithiocarbamato)‐5‐(1, 2, 4‐triazol‐1‐yl)‐3‐propione (2), were prepared by reacting N,N‐dialkyldithiocarbamate sodium with 2, 2‐dimethyl‐4‐bromo‐5‐(1, 2, 4‐triazol‐l‐yl)‐propione. Their structures were identified by elemental analysis, IR and 1H NMR spectroscopy. The structure of 1 has been determined by X‐ray single crystal structure analysis. It crystallizes in monoclinic system with space group P21/c, a=1. 2315(3) nm, b=1.2057(2) nm, c =1.2532(3) nm, β=118.55(3)°, 2=4, V=1.6345(6) nm3, Dc=1.221 g/cm3, p=0.324 mm −1, F(000)=640. final R1 =0.0449. There is obvious potentially weak C‐H···N intermolecular interaction in the crystal, which stabilizes the crystal structure. The result of the biological test showed that the two compounds have fungistasis and plant growth regulating activities.
Different biotinylation strategies for competitive immunoassay of estradiol
Authors: Zhao Jin‐Fu , Wang Yong‐Cheng , Li Yuan‐Zong , Chang Wen‐Bao
Published: September 2010

Study on biotinylation strategies for competitive immunoassay of estradiol (Ez) was carried out. Two types of competitive enzyme immunoassay (WIA) with Biotin‐Avidin amplification system were established and optimized. The E2‐Biotin conjugate was used as a mere in one assay, and biotinylated antibody was used as a tracer in the other. In both of EIAs, horseradish peroxidase‐labelled Avidin (Avidin‐HRP) was used with a spectrometric determination of enzyme activity. The precision, sensitivity and specificity were measured and compared. The results showed that although both were satisfactory in specificity, the EL4 with hapten‐Biotin showed to be superior to the EL4 with biotinylated antibody in sensitivity and precision. The limit of detection of serum E2 was 8 and 50 pg/mL with E2‐Biotin and biotinylated antibody as tracer, respectively.
Study on the reaction of proteins with 5′‐nitrosalicylfluorone‐molybdenum(VI) complex by spectrophotometry in PVA 124 microemulsion
Authors: Wei Qin , Wu Dan , Du Bin , Zhang Hui , Ou Qing‐Yu
Published: September 2010

The application of 5′‐nitrosalicylfluorone (5′‐NSF)‐molybdenum(VI) complex as a spectroscopic probe was studied. In the buffer medium of HOAc‐NaOAc at 3.45 and in the presence of PVA 124 microemulsion, 5 '‐NSF‐Mo(VI) complex combines protein rapidly to form a stable compound. leading to an absorbance decrease at 525 nrn of 5′‐NSF‐Mo(VI) complex. According to this change, microdetermination of protein has been described. Bovine serum albumin (BSA) could be determined in the linear range of 0–16 pg*mL with the detection limit of 11 ng*mL−1. Many amino acid and metal ions studied do not interfere with the assay. The method possesses high sensitivity as well as high selectivity. It can be used to determine protein in human urine and milk powder successfully. The relative standard deviations are in all instances less than 4.7%. and the recoveries are between 97.6% and 106.0%. Moreover, the binding number of BSA with the complex, which is determined by using molar ratio, Rosenthal graphic and slope ratio methods, is in good agreement with each other.