bluuebunny/crossref_metadata_2025 huggingface dataset:
mRNA-based SARS-CoV-2 Comirnaty vaccine elicits weak and short specific memory B cell response in individuals with no previous infection
Authors: Casado José L. , Vizcarra Pilar , Martín-Hondarza Adrián , Gómez-Maldonado Sandra , Muedra-Sánchez Magdalena , del Pino Judith , Mirabella Itria G. , Martín-Colmenarejo Sara , Haemmerle Johannes , Fernández-Escribano Marina , Vallejo Alejandro
Published: June 2023

Objectives

The dynamics of the memory B cell (MBC) repertoire after SARS-CoV-2 vaccination is crucial for assessing long-term immunity. We compare spike-specific MBC responses between SARS-CoV-2 unexposed and recovered individuals, and their impact on breakthrough infections during follow-up.

Methods

Spike-specific MBC and T cells were quantified at inclusion and after two doses of mRNA vaccine in a longitudinal cohort of 85 naïve and 64 recovered participants (47 with positive serology and 17 with negative serology after infection).

Results

At inclusion, there was minimal spike-specific MBC in naïve SARS-CoV-2 individuals. After the second vaccine dose, MBCs were significantly boosted in naïve individuals, but reached a significantly lower level than that observed even in unvaccinated SARS-CoV-2 convalescents (p<0.001). Furthermore, while the secondary memory B cell (MBC) population consisted of 100%, 33%, and 76% IgG+, IgM+, and IgA+ expressing cells, respectively, in the unexposed group, the MBC response showed a significant decrease across all isotypes. Similarly, although secondary specific IgG+, IgM+, and IgA+-MBC isotypes were found in 100%, 39%, and 76% of the unexposed participants, respectively, the magnitude of the MBC levels was significantly lower for all the isotypes compared to convalescents. Interestingly, convalescents without an initial serological response had a lower MBC response, like what found in unexposed subjects. There was an inverse correlation between specific MBCs (r=-0.307; p=0.027), especially for isotype IgA+ (r=-0.279, p=0.045), and the time since the second vaccination dose. Furthermore, during a median follow-up of 434 days (IQR, 339-495), 49 out of 149 individuals (33%) became infected, 29 in naïve and 20 in convalescent individuals, showing a significant correlation between spike-specific MBC magnitude after vaccination and the time for SARS-CoV-2 infection, especially for IgA+/IgG+ MBC isotypes.

Conclusions

MBCs were primed by mRNA-based vaccination in most cases, but SARS-CoV-2 naïve individuals had a blunted specific MBC response, and this was associated with a shorter time to breakthrough SARS-CoV-2 infection.
The intrinsic variability of the Indonesian Throughflow
Authors: Furue Ryo , Nonaka Masami , Sasaki Hideharu
Published: June 2023

The Indonesian Throughflow (ITF) carries an annual average of about 15 Sv of water from the Pacific through the Indonesian Seas Into the Indian Ocean, and its year-to-year variation ranges from 1 to 4 Sv. A 10-member ensemble of 41-year integrations of a semi-global eddy-resolving oceanic general circulation model is examined to explore the intrinsic (chaotic) variability of the ITF transport and associated flow. It is found that the annual-mean ITF transport is different by about 1 Sv between the ensemble members at several years. The characteristic vertical and horizontal structures of the ensemble anomaly (deviation from the ensemble average) are described. These structures and the basin-scale spread of the anomaly suggest that the intrinsic variability of the ITF is a genuine increase or decrease of the classical ITF rather than variability due to local eddies or nonlinear currents within the Indonesian Seas. The lagged correlation of the intrinsic component of the ITF transport with sea-surface height and barotropic streamfunction suggests that the intrinsic variability may come from zonal jets in the western subtropical North Pacific.
Shell accumulation on seabed due to suspended coastal oyster farming and effects on burrowing capacity of the polychaete Perinereis aibuhitensis
Authors: Cao Yujie , Shi Rongjun , Han Tingting , Liu Huaxue , Huang Honghui , Qi Zhanhui
Published: June 2023

Mariculture of bivalves (e.g., mussels, scallops, and oysters) is developing rapidly in coastal waters. This process releases shell debris that can accumulate on the seabed under farms. The ecological consequences of shell accumulation on the bioturbation of benthic macrofauna, which play a key role in the biogeochemical processes of sediment, are not yet fully understood. Thus, it is necessary to quantify the shell debris that accumulated under bivalves farms and evaluate whether the shell debris hinders the burrowing capacity of macrofauna. Oysters are common bivalve species cultured worldwide. However, no data are available on shell accumulation due to oyster farming and its possible effects on the burrowing capacity of polychaetes. Thus, we conducted a preliminary study on shells debris accumulation under an oyster farm in Daya Bay, southern China, and assessed the effects of shell accumulation on the burrowing activity of the polychaete Perinereis aibuhitensis in a mesocosm experiment for 32 days. The results showed that oyster farming significantly (p < 0.05) increased the accumulation of shell debris under the farm. The amount of shell debris under oyster farm (6.0 ± 1.3 kg (dry weight (DW)) m-2 or 130.0 ± 24.7 g (DW) kg-1 dry sediment) was approximately two times that in a non-culture area (3.5 ± 0.9 kg (DW) m-2 or 74.9 ± 10.5 g (DW) kg-1 dry sediment). The presence of shell debris did not affect the survival of P. aibuhitensis in experiment, but the animals in sediment with shell added exhibited greater losses of body weight compared with those without, indicating that the presence of shell causes animals to expend more energy in burrowing. The numbers of burrowing holes were lower in sediment with shell debris added compared with those in sediment without shell addition throughout the experiment. Whereas, this does not due to the P. aibuhitensis in +Shell treatment were less active in terms of their burrowing activities. The lower numbers of holes in +Shell treatment was mainly due to P. aibuhitensis were unable to use surface areas where shells were present, the amount of area occupied by shell material physically prevented P. aibuhitensis from forming burrows in these locations. The hindering effects of shells on the burrowing activity of P. aibuhitensis would decrease as the shells degraded into debris. Therefore, we recommend using small shells when conducting “shell addition” bioremediation strategies to avoid the possible negative effects of shells on burrowing by polychaetes.
IL-1R2-based biomarker models predict melioidosis mortality independent of clinical data
Authors: Kaewarpai Taniya , Wright Shelton W. , Yimthin Thatcha , Phunpang Rungnapa , Dulsuk Adul , Lovelace-Macon Lara , Rerolle Guilhem F. , Dow Denisse B. , Hantrakun Viriya , Day Nicholas P. J. , Lertmemongkolchai Ganjana , Limmathurotsakul Direk , West T. Eoin , Chantratita Narisara
Published: June 2023

Introduction

Melioidosis is an often-fatal tropical infectious disease caused by the Gram-negative bacillus Burkholderia pseudomallei, but few studies have identified promising biomarker candidates to predict outcome.

Methods

In 78 prospectively enrolled patients hospitalized with melioidosis, six candidate protein biomarkers, identified from the literature, were measured in plasma at enrollment. A multi-biomarker model was developed using least absolute shrinkage and selection operator (LASSO) regression, and mortality discrimination was compared to a clinical variable model by receiver operating characteristic curve analysis. Mortality prediction was confirmed in an external validation set of 191 prospectively enrolled patients hospitalized with melioidosis.

Results

LASSO regression selected IL-1R2 and soluble triggering receptor on myeloid cells 1 (sTREM-1) for inclusion in the candidate biomarker model. The areas under the receiver operating characteristic curve (AUC) for mortality discrimination for the IL-1R2 + sTREM-1 model (AUC 0.81, 95% CI 0.72–0.91) as well as for an IL-1R2-only model (AUC 0.78, 95% CI 0.68–0.88) were higher than for a model based on a modified Sequential Organ Failure Assessment (SOFA) score (AUC 0.69, 95% CI 0.56–0.81, p < 0.01, p = 0.03, respectively). In the external validation set, the IL-1R2 + sTREM-1 model (AUC 0.86, 95% CI 0.81–0.92) had superior 28-day mortality discrimination compared to a modified SOFA model (AUC 0.80, 95% CI 0.74–0.86, p < 0.01) and was similar to a model containing IL-1R2 alone (AUC 0.82, 95% CI 0.76–0.88, p = 0.33).

Conclusion

Biomarker models containing IL-1R2 had improved 28-day mortality prediction compared to clinical variable models in melioidosis and may be targets for future, rapid test development.
Mill dams impact microbiome structure and depth distribution in riparian sediments
Authors: Kan Jinjun , Peck Erin K. , Zgleszewski Laura , Peipoch Marc , Inamdar Shreeram
Published: June 2023

Introduction

Damming has substantially fragmented and altered riverine ecosystems worldwide. Dams slow down streamflows, raise stream and groundwater levels, create anoxic or hypoxic hyporheic and riparian environments and result in deposition of fine sediments above dams. These sediments represent a good opportunity to study human legacies altering soil environments, for which we lack knowledge on microbial structure, depth distribution, and ecological function.

Methods

Here, we compared high throughput sequencing of bacterial/ archaeal and fungal community structure (diversity and composition) and functional genes (i.e., nitrification and denitrification) at different depths (ranging from 0 to 4 m) in riparian sediments above breached and existing milldams in the Mid-Atlantic United States.

Results

We found significant location- and depth-dependent changes in microbial community structure. Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Chloroflexi, Acidobacteria, Planctomycetes, Thaumarchaeota, and Verrucomicrobia were the major prokaryotic components while Ascomycota, Basidiomycota, Chytridiomycota, Mortierellomycota, Mucoromycota, and Rozellomycota dominated fungal sequences retrieved from sediment samples. Ammonia oxidizing genes (amoA for AOA) were higher at the sediment surface but decreased sharply with depth. Besides top layers, denitrifying genes (nosZ) were also present at depth, indicating a higher denitrification potential in the deeper layers. However, these results contrasted with in situ denitrification enzyme assay (DEA) measurements, suggesting the presence of dormant microbes and/or other nitrogen processes in deep sediments that compete with denitrification. In addition to enhanced depth stratification, our results also highlighted that dam removal increased species richness, microbial diversity, and nitrification.

Discussion

Lateral and vertical spatial distributions of soil microbiomes (both prokaryotes and fungi) suggest that not only sediment stratification but also concurrent watershed conditions are important in explaining the depth profiles of microbial communities and functional genes in dammed rivers. The results also provide valuable information and guidance to stakeholders and restoration projects.
Profiles and spatial distributions of heavy metals, microbial communities, and metal resistance genes in sediments from an urban river
Authors: Fu Lingfang , Yu Yang , Yu Fei , Xiao Jieer , Fang Huaiyang , Li Weijie , Xie Zhijie , Zhang Feng , Lin Shu
Published: June 2023

The occurrence and propagation of resistance genes due to exposure to heavy metals (HMs) in rivers is an emerging environmental issue. Little is known about resistance genes in microbial communities in river sediments with low HM concentrations. The profiles and spatial distributions of HMs, the microbial community, and metal resistance genes (MRGs) were analyzed in sediment samples from the Zhilong River basin in Yangjiang city, near the Pearl River Delta. Concentrations of copper (Cu), cadmium (Cd), lead (Pb), chromium (Cr), and nickel (Ni) were relatively low compared with those in other urban river sediments in China. HM chemical composition and fractions and the structure of the microbial community varied along the main channel, but the composition and abundance of MRGs were relatively homogeneous. Variations in HMs and microbial communities in mid- to upstream areas were related to the presence of tributaries, whose inputs were one of the major factors affecting HM chemical fractions and genera structure in mainstream sediments. There were no significant correlations (p < 0.05) between HM concentrations, bacterial communities, and the MRG profiles; thus, HM concentrations were not the main factor affecting MRGs in sediments. These results contribute to understanding the propagation of MRGs in urban rivers in developing cities.
Dynamic allostery in thrombin—a review
Authors: Komives Elizabeth A.
Published: June 2023

Thrombin is a serine protease that catalyzes a large number of different reactions including proteolytic cleave of fibrinogen to make the fibrin clot (procoagulant activity), of the protease activated receptors (for cell signaling) and of protein C generating activated protein C (anticoagulant activity). Thrombin has an effector binding site called the anion binding exosite 1 that is allosterically coupled to the active site. In this review, we survey results from thermodynamic characterization of the allosteric coupling as well as hydrogen-deuterium exchange mass spectrometry to reveal which parts of the thrombin structure are changed upon effector binding and/or mutagenesis, and finally NMR spectroscopy to characterize the different timescales of motions elicited by the effectors. We also relate the experimental work to computational network analysis of the thrombin-thrombomodulin complex.
Effects of Tai Chi on working memory in older adults: evidence from combined fNIRS and ERP
Authors: Wang Chen , Dai Yuanfu , Yang Yuan , Yuan Xiaoxia , Zhang Mengjie , Zeng Jia , Zhong Xiaoke , Meng Jiao , Jiang Changhao
Published: June 2023

Objective

The study aimed to investigate the effects of a 12-week Tai Chi exercise intervention on working memory in older adults using ERP-fNIRS.

Method

Fifty older adults were randomly assigned to either an experimental group receiving a 12-week Tai Chi exercise intervention or a control group receiving regular daily activities. Working memory was assessed using the n-back task before and after the intervention, and spatial and temporal components of neural function underlying the n-back task were measured using ERP-fNIRS.

Results

The experimental group demonstrated significant improvements in reaction time and accuracy on the 2-back task and showed higher activation levels in the R-DLPFC. Additionally, the Tai Chi group displayed significant increases in P3 amplitude in the overall n-back task.

Conclusion

These findings suggest that Tai Chi interventions can enhance working memory in older adults, as evidenced by increasing neural activity and improving HbO in the R-DLPFC during the 2-back task.
Long-term outcomes of coils embolization for superior hypophyseal artery aneurysms
Authors: Kang Yan-Po , Li Cheng-Yu , Chen Chun-Ting , Yeap Mun-Chun , Wong Ho-Fai , Wu Yi-Ming , Hsieh Po-Chuan , Liu Zhuo-Hao , Chuang Chi-Cheng , Chen Ching-Chang
Published: June 2023

Objective

Superior hypophyseal artery (SHA) aneurysms are intradural, and their rupture can result in subarachnoid hemorrhage. Considering the related surgical difficulty and anatomical restrictions, endovascular treatment (EVT) is considered the most favorable modality for SHA aneurysms; however, the long-term outcomes of EVT have rarely been reported. The study assessed the incidence of and risk factors for recurrence of SHA aneurysms after EVT as well as the correlation factors for SHA aneurysm rupture.

Methods

We included 112 patients with SHA aneurysms treated with EVT at our facility between 2009 and 2020. Here, EVT included non–stent-assisted (simple or balloon-assisted) or stent-assisted coiling. Flow diverter was not included because it was barely used due to its high cost under our national insurance’s limitation, and a high proportion of ruptured aneurysms in our series. Univariate and multivariate logistic regression was performed to evaluate the correlation factors for SHA aneurysm rupture, along with the incidence of and risk factors for post-EVT SHA aneurysm recurrence and re-treatment.

Results

In our patients, the mean angiographic follow-up period was 3.12 years. The presence of type IA or IB cavernous internal carotid artery (cICA) was strongly correlated with SHA aneurysm rupture. Recurrence occurred in 17 (13.4%) patients, of which only 1 (1.4%) patient had received stent-assisted coiling. All cases of recurrence were observed within 2 years after EVT. The multivariate logistic regression results showed that ruptured aneurysm and non–stent-assisted coiling were independent risk factors for aneurysm recurrence. Of the 17 cases of aneurysm recurrence, 9 (52.9%) received re-treatment. Moreover, aneurysm rupture was the only factor significantly correlated with re-treatment in multivariate logistic regression. No re-recurrence was observed when a recurrent aneurysm was treated with stent-assisted coiling.

Conclusion

Type I cICA was common factor for aneurysm rupture. Although flow-diverter treatment serves as another suitable technique that was not compared with, coils embolization was effective treatment modality for SHA aneurysms, leading to low recurrence and complication rates, especially with stent use. All cases of recurrence occurred within 2 years after EVT; they were strongly associated with prior aneurysm rupture. Further stent-assisted coiling was noticed to prevent re-recurrence.
The analysis of SUDEP forensic autopsies leading to preventable events
Authors: Argo Antonina , Puntarello Maria , Malta Ginevra , Buscemi Roberto , Scalzo Giovanni , Triolo Valentina , Albano Giuseppe Davide , Zerbo Stefania
Published: June 2023

Introduction

The diagnosis of unexpected death by excluding non-natural causes, particularly in subjects with epilepsy, is a topic of interest and it is difficult to identify in the forensic field. Health professionals sometimes are faced with cases of sudden death, generally in young adults with a long history of epilepsy that require, for judicial purposes, an explanation in terms of cause and means to determine the death. SUDEP is an entity diagnosed by the exclusion of other causes that may have led to death, and then for forensic purposes, it requires particular attention and knowledge, and there is difficulty in identifying it. Our contribution aims to illustrate the scientific community pathological findings, medical history, and circumstantial evidence of four cases of sudden death in epileptic subjects.

Method

We illustrated four cases of judicial autopsies from the Institute of Forensic Medicine of Palermo, Italy; the purpose was to exclude the criminal intervention in determining the death as non-natural. The study of victims’ medical history, the toxicological investigations, and the autopsy findings analyzed both from macroscopic and microscopic aspects have made it possible to highlight some findings that can be traced back to SUDEP despite the small sample of subjects studied.

Results

These presented findings of four SUDEP cases could help forensic pathologists in recognizing this entity, by highlighting its characteristics, and allowing for a pathological classification, also in relation to the use of drugs for epilepsy treatment and circumstances of death.

Discussion

To obtain a definite diagnosis of SUDEP, a complex investigation process is required in a multidisciplinary approach. Considering the literature review with criticism, it could allow health professionals to select the characteristics of epileptic patients at risk of sudden death. Processing human behaviors, molecular and histopathological findings of the autopsies, but also the physiological, and pathological human body system functions thanks to Artificial Intelligence, could be the key to explaining SUDEP mechanisms and the future results to prevent it.
Olfaction and neurocognition after COVID-19: a scoping review
Authors: Vilarello Brandon J. , Jacobson Patricia T. , Tervo Jeremy P. , Waring Nicholas A. , Gudis David A. , Goldberg Terry E. , Devanand D. P. , Overdevest Jonathan B.
Published: June 2023

Introduction

COVID-19 induces both acute and chronic neurological changes. Existing evidence suggests that chemosensory changes, particularly olfactory loss, may reflect central neurological dysfunction in neurodegenerative diseases and mark progression from mild cognitive impairment to Alzheimer’s. This scoping review summarizes the available literature to evaluate the relationship between neurocognition and olfaction in young to middle-aged adults with minimal comorbidities following COVID-19 infection.

Methods

A literature search of PubMed, Ovid Embase, Web of Science, and Cochrane Library was conducted. Studies underwent title/abstract and full text screening by two reviewers, with a third reviewer resolving any conflicts. Remaining studies underwent data extraction.

Results

Seventeen studies were eligible for data extraction after the review process, where 12 studies found significantly poorer cognition in those suffering from olfactory dysfunction, four studies showed no association between cognition and olfaction, and one study reported lower anosmia prevalence among patients with cognitive impairment.

Conclusion

The majority of studies in this review find that olfactory dysfunction is associated with poorer cognition. More rigorous studies are needed to further elucidate the relationship between olfaction and cognition after COVID-19.
An optimized temporally controlled Gal4 system in Drosophila reveals degeneration caused by adult-onset neuronal Vps13D knockdown
Authors: Rozich Emily , Randolph Lynsey K. , Insolera Ryan
Published: June 2023

Mutations in the human gene VPS13D cause the adult-onset neurodegenerative disease ataxia. Our previous work showed that disruptions in the Vps13D gene in Drosophila neurons causes mitochondrial defects. However, developmental lethality caused by Vps13D loss limited our understanding of the long-term physiological effects of Vps13D perturbation in neurons. Here, we optimized a previously generated system to temporally knock down Vps13D expression precisely in adult Drosophila neurons using a modification to the Gal4/UAS system. Adult-onset activation of Gal4 was enacted using the chemically-inducible tool which fuses a destabilization-domain to the Gal4 repressor Gal80 (Gal80-DD). Optimization of the Gal80-DD tool shows that feeding animals the DD-stabilizing drug trimethoprim (TMP) during development and rearing at a reduced temperature maximally represses Gal4 activity. Temperature shift and removal of TMP from the food after eclosion robustly activates Gal4 expression in adult neurons. Using the optimized Gal80-DD system, we find that adult-onset Vps13D RNAi expression in neurons causes the accumulation of mitophagy intermediates, progressive deficits in locomotor activity, early lethality, and brain vacuolization characteristic of neurodegeneration. The development of this optimized system allows us to more precisely examine the degenerative phenotypes caused by Vps13D disruption, and can likely be utilized in the future for other genes associated with neurological diseases whose manipulation causes developmental lethality in Drosophila.
Physiological synchrony in electrodermal activity predicts decreased vigilant attention induced by sleep deprivation
Authors: Stuldreher Ivo V. , Maasland Emma , Bottenheft Charelle , van Erp Jan B. F. , Brouwer Anne-Marie
Published: June 2023

Introduction

When multiple individuals are presented with narrative movie or audio clips, their electrodermal activity (EDA) and heart rate show significant similarities. Higher levels of such inter-subject physiological synchrony are related with higher levels of attention toward the narrative, as for instance expressed by more correctly answered questions about the narrative. We here investigate whether physiological synchrony in EDA and heart rate during watching of movie clips predicts performance on a subsequent vigilant attention task among participants exposed to a night of total sleep deprivation.

Methods

We recorded EDA and heart rate of 54 participants during a night of total sleep deprivation. Every hour from 22:00 to 07:00 participants watched a 10-min movie clip during which we computed inter-subject physiological synchrony. Afterwards, they answered questions about the movie and performed the psychomotor vigilance task (PVT) to capture attentional performance.

Results

We replicated findings that inter-subject correlations in EDA and heart rate predicted the number of correct answers on questions about the movie clips. Furthermore, we found that inter-subject correlations in EDA, but not in heart rate, predicted PVT performance. Individuals' mean EDA and heart rate also predicted their PVT performance. For EDA, inter-subject correlations explained more variance of PVT performance than individuals' mean EDA.

Discussion

Together, these findings confirm the association between physiological synchrony and attention. Physiological synchrony in EDA does not only capture the attentional processing during the time that it is determined, but also proves valuable for capturing more general changes in the attentional state of monitored individuals.
Knowledge, attitudes, and practices of registered dietitians and nutritionists regarding enteral and parenteral nutrition support in Ghana: a needs assessment study
Authors: Ayande Ruthfirst E. A. , Agordoh Percival D. , Salino Vanessa J. , Webster-Ariyan Clarisa , Collier Lindsay , Asante Matilda , Carbone Elena T.
Published: June 2023

Background

There is a paucity of data on enteral and parenteral (EN and PN) nutrition support (NS) provided by nutrition and dietetic practitioners in adult acute care settings in Ghana. Furthermore, gray literature suggests that Ghanaian clinical nutrition professionals (CNPs) are seldom involved in advanced nutrition care teams.

Objectives

To assess the knowledge, attitudes, and practices of Ghanaian CNPs regarding EN and PN.

Methods

An online cross-sectional survey was administered to Ghanaian CNPs ahead of a professional development workshop on EN and PN Support. Participants were asked questions about initiation and timing of NS, and knowledge on availability of commercial formula. A 5-point Likert scale was used to assess self-efficacy in using EN and PN. To assess practical knowledge on EN and PN, participants were asked to identify whether EN or PN was indicated for seven short case scenarios. Open-ended questions were used to assess reasons for participant self-ratings.

Results

A total of 76 dietitians, nutritionists, students, and interns completed the survey. For EN, self-efficacy scores were lowest for the calculation of enteral goal rate, and goal volume (mean 3.20 ± 1.27), and writing of EN prescriptions (mean 3.07 ± 1.29). Self-efficacy scores for the formulation of alternative formulas in lieu of commercial formula were the highest (3.63 ± 1.36). For PN, self-efficacy scores for all domains were lower than 3, with the lowest scores observed for writing PN prescriptions (2.19 ± 1.14) and determining micronutrient additives (2.12 ± 1.04). We identified limited training and lack of practical exposure to NS, limited ability to effectively monitor tube feeds, and prohibitive cost and limited availability of EN and PN formula among the barriers impacting self-efficacy scores.

Conclusion

Given the vital role that CNPs play in the delivery of EN and PN, it is important to develop professional training programs especially focused on PN to bridge knowledge and practice gaps.
Survival benefit of preoperative hepatic arterial infusion of oxaliplatin, fluorouracil, and leucovorin followed by hepatectomy for hepatocellular carcinoma
Authors: Hu Zili , Yang Zhenyun , Pan Yangxun , Fu Yizhen , Wang Jiongliang , Zhou Zhongguo , Chen Minshan , Hu Dandan , Zhang Yaojun
Published: June 2023

Background: Hepatic arterial infusion chemotherapy (HAIC) with cisplatin, fluorouracil, and leucovorin (FOLFOX) demonstrated promising efficacy against advanced hepatocellular carcinoma (HCC) as an alleviative treatment. We aimed to explore the survival benefit of preoperative FOLFOX-HAIC and establish a predictive nomogram.

Methods: This study retrospectively reviewed data from 1251 HCC patients who underwent liver resection. 1027 patients received liver resection alone (LR group), and 224 patients were treated with FOLFOX-HAIC followed by liver resection (HLR group). Propensity score matching (PSM) was conducted between the two groups. The nomogram was established based on the findings of the multivariable Cox regression analysis.

Results: After Propensity score matching according to initial tumor characteristics, the 1-, 2-, and 3-year overall survival rates were 85.4, 72.0, and 67.2% in the LR group and 95.2, 84.7, and 75.9% in the HLR group, respectively (p = 0.014). After PSM according to preoperative tumor characteristics, the 1-, 2-, and 3-year OS rates were 87.9, 76.6, and 72.3% in the LR group and 95.4, 84.4, and 75.1% in the HLR group, respectively (p = 0.24). Harrell’s C-indexes of the nomogram for OS prediction in patients with preoperative FOLFOX-HAIC were 0.82 (95% CI 0.78–0.86) in the training cohort and 0.87 (95% CI 0.83–0.93) in the validation cohort and the nomogram performed well-fitted calibration curves.

Conclusion: Preoperative FOLFOX-HAIC is associated with a longer survival outcome for HCC patients. The novel nomogram efficiently predicted the OS of patients who underwent preoperative FOLFOX-HAIC.
A novel colchicine-myricetin heterozygous molecule: design, synthesis, and effective evaluations on the pathological models of acute lung injury in vitro and in vivo
Authors: Li Zhiyue , Yan Xueqin , Wei Jiangchun , Pu Liuyang , Zhu Guanbao , Cao Yongkai , Liu Zhanyan , Liu Yaqian , Li Yan , Li Limin , Li Xinping , Wu Zhengzhi
Published: June 2023

Acute lung injury (ALI) is an inflammatory condition and there are no effective treatments. A novel new compound----colchicine-myricetin hybrid (CMyrH) was herein designed and synthesized. To evaluate the activity of CMyrH in ALI, we used a bleomycin (BLM) induced BEAS-2B injury model in vitro and established a well-recognized rat model of BLM-induced lung injury in vivo. The results demonstrated that colchicine-myricetin hybrid protected BEAS-2B cells against BLM-induced cell injury in an increased dose manner, and reduced wet/dry weight ratio, histological scoring, and inflammation cytokines IL-1β, IL-6, IL-18, and TNF-α levels of lung tissue of the rats. Furthermore, we found colchicine-myricetin hybrid inhibited caspase-1, ASC, GSDMD, and NLRP-3 expression in vivo. Meanwhile, we used molecular docking to analyze the binding mode of colchicine-myricetin hybrid and human neutrophil elastase (HNE), it revealed that colchicine-myricetin hybrid showed strong binding affinity toward human neutrophil elastase when compared to its parent molecules. In conclusion, It is suggested that colchicine-myricetin hybrid antagonized acute lung injury by focusing on multi-targets via multi-mechanisms, and might be served as a potential therapeutic agent for acute lung injury.
Toward a 3D physical model of diffusive polymer chains
Authors: Karsai Andras , Cassidy Grace J. , Rajanala Aradhya P. , Yang Lixinhao , Kerimoglu Deniz , Gumbart James C. , Kim Harold D. , Goldman Daniel I.
Published: June 2023

Recent studies in polymer physics have created macro-scale analogs to solute microscopic polymer chains like DNA by inducing diffusive motion on a chain of beads. These bead chains have persistence lengths of O(10) links and undergo diffusive motion under random fluctuations like vibration. We present a bead chain model within a new stochastic forcing system: an air fluidizing bed of granular media. A chain of spherical 6 mm resin beads crimped onto silk thread are buffeted randomly by the multiphase flow of grains and low density rising air “bubbles”. We “thermalize” bead chains of various lengths at different fluidizing airflow rates, while X-ray imaging captures a projection of the chains’ dynamics within the media. With modern 3D printing techniques, we can better represent complex polymers by geometrically varying bead connections and their relative strength, e.g., mimicking the variable stiffness between adjacent nucleotide pairs of DNA. We also develop Discrete Element Method (DEM) simulations to study the 3D motion of the bead chain, where the bead chain is represented by simulated spherical particles connected by linear and angular spring-like bonds. In experiment, we find that the velocity distributions of the beads follow exponential distributions rather than the Gaussian distributions expected from polymers in solution. Through use of the DEM simulation, we find that this difference can likely be attributed to the distributions of the forces imparted onto the chain from the fluidized bed environment. We anticipate expanding this study in the future to explore a wide range of chain composition and confinement geometry, which will provide insights into the physics of large biopolymers.
Molecular imaging of inflammation with PET in acute and ventilator-induced lung injury
Authors: Musch Guido
Published: June 2023

This review focuses on methods to image acute lung inflammation with Positron Emission Tomography (PET). Four approaches are discussed that differ for biologic function of the PET reporter probe, radiotracer employed, and the specific aspect of the inflammatory response that is targeted. 2-[18F]fluoro-2-deoxy-D-glucose ([18F]FDG) is an enzyme substrate whose uptake is used to measure the metabolic activation of inflammatory cells during acute lung injury in the noncancerous lung. H215O and radiolabeled plasma proteins are inert molecules with the same physical characteristics as their nonradioactive counterparts and are used to measure edema and vascular permeability. Tagged enzyme or receptor inhibitors are used to probe expression of these targets induced by inflammatory stimuli. Lastly, cell-specific tracers are being developed to differentiate the cell types that contribute to the inflammatory response. Taken together, these methods cast PET imaging as a versatile and quantitative tool to measure inflammation in vivo noninvasively during acute and ventilator-induced lung injury.
Transdermal tibial nerve optogenetic stimulation targeting C-fibers
Authors: Zhou Zhonghan , Wang Xuesheng , Li Xunhua , Liao Limin
Published: June 2023

Purpose: To explore whether stimulation of C-fibers in tibial nerves can induce bladder inhibition by optogenetic transdermal illumination.

Methods: Ten rats were injected with AAV2/6-hSyn-ChR2(H134R)-EYFP into the tibial nerves. Transurethral cystometry was performed 4 weeks after the virus injection. Illumination (473-nm blue light at 100 mW) was performed with the fiber positioned above the right hind paw near the ankle. The light transmission efficiency was examined with a laser power meter. The effects on cystometry were compared before and after illumination with the bladder infused with normal saline and acetic acid, respectively.

Result: Upon transdermal delivery of 473-nm light at a peak power of 100 mW, the irradiance value of 0.653 mW/mm2 at the target region was detected, which is sufficient to activate opsins. The photothermal effect of 473-nm light is unremarkable. Acute inhibitory responses were not observed during stimulation regarding any of the bladder parameters; whereas, after laser illumination for 30 min, a statistically significant increase in bladder capacity with the bladder infused with normal saline (from 0.53 ± 0.04 mL to 0.72 ± 0.05 mL, p < 0.001) and acetic acid (from 0.25 ± 0.02 mL to 0.37 ± 0.04 mL, p < 0.001) was detected. A similar inhibitory response was observed with pulsed illumination at both 10Hz and 50Hz. However, illumination did not significantly influence base pressure, threshold pressure, or peak pressure.

Conclusion: In this preliminary study, it can be inferred that the prolonged bladder inhibition is mediated by the stimulation of C-fibers in the tibial nerves, with no frequency-dependent characteristics. Although the 473-nm blue light has limited penetration efficacy, it is sufficient to modulate bladder functions through transdermal illumination on the superficial peripheral nervous system.
Resilient, but for how long? The relationships between temperament, burnout, and mental health in healthcare workers during the Covid-19 pandemic
Authors: Gertler Joshua , Dale Lourdes , Tracy Natasha , Dorsett Joelle , Sambuco Nicola , Guastello Andrea , Allen Brandon , Cuffe Steven P. , Mathews Carol A.
Published: June 2023

Introduction

Dispositional traits of wellbeing and stress-reaction are strong predictors of mood symptoms following stressful life events, and the COVID-19 pandemic introduced many life stressors, especially for healthcare workers.

Methods

We longitudinally investigated the relationships among positive and negative temperament group status (created according to wellbeing and stress-reaction personality measures), burnout (exhaustion, interpersonal disengagement), COVID concern (e.g., health, money worries), and moral injury (personal acts, others’ acts) as predictors of generalized anxiety, depression, and post-traumatic stress symptoms in 435 healthcare workers. Participants were employees in healthcare settings in North Central Florida who completed online surveys monthly for 8 months starting in October/November 2020. Multidimensional Personality Questionnaire subscale scores for stress-reaction and wellbeing were subjected to K-means cluster analyses that identified two groups of individuals, those with high stress-reaction and low wellbeing (negative temperament) and those with the opposite pattern defined as positive temperament (low stress-reaction and high wellbeing). Repeated measures ANOVAs assessed all time points and ANCOVAs assessed the biggest change at timepoint 2 while controlling for baseline symptoms.

Results and Discussion

The negative temperament group reported greater mood symptoms, burnout, and COVID concern, than positive temperament participants overall, and negative participants’ scores decreased over time while positive participants’ scores increased over time. Burnout appeared to most strongly mediate this group-by-time interaction, with the burnout exhaustion scale driving anxiety and depression symptoms. PTSD symptoms were also related to COVID-19 health worry and negative temperament. Overall, results suggest that individuals with higher stress-reactions and more negative outlooks on life were at risk for anxiety, depression, and PTSD early in the COVID-19 pandemic, whereas individuals with positive temperament traits became more exhausted and thus more symptomatic over time. Targeting interventions to reduce mood symptoms in negative temperament individuals and prevent burnout/exhaustion in positive temperament individuals early in an extended crisis may be an efficient and effective approach to reduce the mental health burden on essential workers.
Economic burden of sickle cell disease in Sweden: a population-based national register study with 13 years follow up
Authors: Kjellander Christian , Hernlund Emma , Ivergård Moa , Svedbom Axel , Dibbern Therese , Stenling Anna , Sjöö Fredrik , Vertuani Simona , Glenthøj Andreas , Cherif Honar
Published: June 2023

Introduction

Sickle cell disease (SCD) describes a group of inherited disorders of hemoglobin. Globally, SCD occurs in approximately 300,000-400,000 births annually and is most prevalent in malaria-endemic countries. However, migration has impacted the epidemiology of SCD but data on the matter are scarce. The objective of this study was to describe the epidemiology, treatment uptake, and economic burden of SCD in Sweden, a country with substantial immigration over the last decades.

Methods

This nationwide retrospective observational registry cohort study identified patients with SCD from 2001 to 2018 and followed them from 2006 to 2018. Using data from high-quality population-based Swedish registers, we estimated prevalence, treatment uptake, and SCD-related health care resource use, sick leave and disability pension.

Results

Between 2006 and 2018 the number of patients with SCD increased from 504 to 670; inpatient hospital stays and outpatient visits increased by 200% and 300%, respectively. Patients with pain crises had approximately twice the number of inpatient episodes and outpatient visit per year, and had higher productivity losses compared to patients without crises.

Conclusion

In an era of emerging treatments for SCD, we have, to the best of our knowledge, for the first time comprehensively described epidemiological and economic aspects of SCD in a country where the disease is still rare and not well recognized by the healthcare system.
Environmental movements in a material world. A relational perspective on single-use plastic in Penzance, UK
Authors: Idies Yusif , Mössner Samuel
Published: June 2023

In the discursive battle about the big and important ideas, some topics are considered more important than others and more likely to mobilize society. More recently, marine plastic pollution has become a key topic for environmental movements. In this study, we aimed to contribute to a more detailed understanding of how specific issues of sustainability are brought forth. Conceptually, we argue that recourse to relational theories from science and technology studies might help to strengthen the conceptualization of social movement research. By drawing on the concept of “boundary objects”, we extend the range of social movement approaches in order to better understand how the process of framing is put to work materially. The focus on boundary objects can therefore explain the selective formation of environmental discourses and policies when applied to the field of environmental protection and sustainability.
A tri-band terrestrial beacon digital receiver system for TEC measurements
Authors: Zhang Chu-Yuan , Zhao Rong-Bing , Zhang Dong , Yu Dong-Mei , Wu Ya-Jun , Shang-Guan Wei-Hua , Yan Zhen
Published: June 2023

The ionosphere is an important component of near-Earth space that has a significant impact on communication and navigation. Furthermore, the total electron content (TEC) of the ionosphere is a widely used parameter in near-Earth plasma environment studies. A design of a digital beacon receiver system for automatically measuring TEC is presented. Universal Software Radio Peripheral (USRP) hardware equipment with software-defined radio technology was used in the tri-band beacon digital receiver to receive and rapidly preprocess beacon signals from satellites in different frequency bands. A software system is built to implement the automatic control of the receiver and to enable data processing of the preprocessed data in order to obtain its phase information and derive the TEC. The tri-band digital beacon receiver system is experimentally proven to automatically detect and archive transit satellites in real time, automatically receive and process satellite beacon signals with recording, and automatically generate TEC measurement results.
Streaming the beautiful game: exploring big tech's growing presence in the soccer industry
Authors: Fakataulavelua Alexis , Lang Markus , Moulard Jérémy
Published: June 2023

This study investigates the evolving role of major technology corporations—namely, Amazon, Apple, Microsoft, Facebook, Netflix, and Google—in the sports industry, with a specific focus on soccer. By employing a qualitative content analysis of media reports, scientific literature, and annual reports from 2000 to 2021, the research scrutinizes the varying approaches and investments of these tech giants in the domain of sports. The findings classify these companies into three distinct categories: (1) those actively securing broadcast rights for major competitions and leagues (Google, Facebook, Amazon); (2) those primarily producing and disseminating soccer documentaries (Netflix); and (3) those not directly engaging in media rights but advancing the technological aspects of clubs and leagues (Apple and Microsoft). This study underscores the escalating significance of Big Tech in reshaping the sports media landscape and calls for further research to comprehend the broader implications of their presence in sports broadcasting and fan engagement.
Modeling and Experimental Validation of the Performance of Electromechanical Height Adjustment Vehicle Suspension with Eccentric Mounted Screw System
Authors: Ruzimov Sanjarbek , Castellanos Molina Luis M. , Galluzzi Renato , Manca Raffaele , Amati Nicola , Tonoli Andrea
Published: June 2023

This paper describes the modeling and experimental validation of the performance of two height adjustment suspensions with concentrically and eccentrically mounted screws. In the former solution, an anti-rotation system is required for the generation of reaction torque on the power screw–nut mechanism. The anti-rotation represents the main drawback of such mechanisms. In contrast, the eccentric solution attempts to solve this problem by placing the screw–nut mechanism eccentrically with respect to the shock absorber tube axis. In this paper, the working principle of the eccentric solution is explained. Its performance is compared to the concentric counterpart through simulations and experiments. Although the efficiencies of eccentric and concentric systems are very similar at the power screw, overall efficiencies differ substantially. During lifting, average efficiencies are around 3.4% and 6.5% for concentric and eccentric systems, respectively. When lowering, these values are 6.2% and 26%. The higher overall efficiency of the eccentric screw system is attributed to the anti-rotation system and the balancing of the bending moment due to the offset application of the load. To yield a complete perspective on the eccentric mounted screw solution, four prototypes are installed and tested on a demo vehicle.
Special Issue “Aeroacoustics and Noise Mitigation”
Authors: Yao Hua-Dong
Published: June 2023

Aerospace, an open access journal operated by MDPI, recently released a Special Issue entitled “Aeroacoustics and Noise Mitigation” [...]