bluuebunny/crossref_metadata_2025 huggingface dataset:
Matter Flow Through an Animal Model Feed With Grasshopper Sphenarium purpurascens: Evidence of a Sustainable and Nutritious Protein Production System
Authors: Meza-Cureño Lluvia Topacio , Mendieta Sánchez Adela Monserrat , Castillo Ana Maria , Cabello Hernandez Christian , Carmona Agustin , Alavez Valeria , Martínez Yuriana , Garcia-Cuenca Edilberto , Cano-Santana Zenón , Cerritos René
Published: February 2022

The demand for food and the environmental damage linked to its production will increase significantly; thus, the need for protein sources that are nutritious, digestible, and sustainable in environmental terms is rising. Many bromatological studies show that insects possess high protein amounts, becoming an alternative to conventional livestock. However, in vivo studies on digestibility are scarce (either on humans or laboratory animals), particularly when analyzing assimilation efficiency. In this work, we measured weight gain, food intake, feces and urine quantification, protein assimilation efficiency, and food conversion ratio in Wistar rats fed with five different diets for 5 weeks. Three diets were based on different concentrations of orthopteran protein (Sphenarium purpurascens) and were compared against a control diet and a highly caloric treatment, which was heavy in sugar and refined flours. The diet based on 23% of S. purpurascens protein resulted in 2.0 and 20.8% more biomass accumulation than the control and the highly caloric diet, respectively. The diets with 18% and 23% insect protein exhibited an average feed conversion of 6.3, while the control group resulted in 8.5 and the caloric diet in 42.7. These results show that S. purpurascens protein yields higher or equal conversion values than a conventional diet, indicating that grasshoppers can be a sustainable and nutritious food for humans and an alternative to livestock. On the other hand, even if feeding insects to farm animals for human consumption is considered an option, it seems highly inefficient, mainly because this practice increases one trophic level in the energy flow. Therefore, incorporating insect protein into human diets represents a sustainable alternative to face future feeding and environmental challenges. Proposals like the one we present here have yet to be evaluated; however, our findings in laboratory animals project a promising future for human populations, where low environmental cost proteins could be obtained sustainably.
Non-intubated Thoracoscopic Surgery-Tips and Tricks From Anesthesiological Aspects: A Mini Review
Authors: Fabo Csongor , Oszlanyi Adam , Lantos Judit , Rarosi Ferenc , Horvath Theodor , Barta Zsanett , Nemeth Tibor , Szabo Zsolt
Published: February 2022

Background

In the last few decades, surgical techniques have been developed in thoracic surgery, and minimally invasive strategies such as multi-and uniportal video-assisted thoracic surgery (VATS) have become more favorable even for major pulmonary resections. With this surgical evolution, the aesthetic approach has also changed, and a paradigm shift has occurred. The traditional conception of general anesthesia, muscle relaxation, and intubation has been re-evaluated, and spontaneous breathing plays a central role in our practice by performing non-intubated thoracoscopic surgeries (NITS-VATS).

Methods

We performed a computerized search of the medical literature (PubMed, Google Scholar, Scopus) to identify relevant articles in non-intubated thoracoscopic surgery using the following terms [(non-intubated) OR (non-intubated) OR (awake) OR (tubeless) OR (regional anesthesia)] AND [(VATS) OR (NIVATS)], as well as their Medical Subject Headings (MeSH) terms.

Results

Based on the outcomes of the reviewed literature and our practice, it seems that pathophysiological concerns can be overcome by proper surgical and anesthetic management. All risks are compensated by the advantageous physiological changes that result in better patient outcomes. With the maintenance of spontaneous breathing, the incidence of potential adverse effects of mechanical ventilation, such as ventilator-induced lung injury and consequent postoperative pulmonary complications, can be reduced. The avoidance of muscle relaxants also results in the maintenance of contraction of the dependent hemidiaphragm and lower airway pressure levels, which may lead to better ventilation-perfusion matching. These techniques can be challenging for surgeons as well as for anesthetists; hence, a good knowledge of physiological and pathophysiological changes, clear inclusion and exclusion and intraoperative conversion criteria, and good communication between team members are essential.

Conclusion

NITS-VATS seems to be a feasible and safe method in selected patients with evolving importance as a part of the minimally invasive surgical and anesthetic conception and has a role in reducing perioperative complications, which is crucial in the thoracic surgical patient population.
Biological Characteristics and Pathogenicity of Helcococcus ovis Isolated From Clinical Bovine Mastitis in a Chinese Dairy Herd
Authors: Liu Kai , Deng Zhaoju , Zhang Limei , Gu Xiaolong , Liu Gang , Liu Yang , Chen Peng , Gao Jian , Han Bo , Qu Weijie
Published: February 2022

Helcococcus ovis (H. ovis) was first reported in ovine subclinical mastitis milk and post-mortem examination organs in Spain and the United Kingdom in 1999; subsequently, it appeared in cattle, horse, goat, and human. However, isolation and characterization of the pathogen from clinical bovine mastitis is unknown. The objective of this study was to identify the pathogen in clinical bovine mastitis. A total of four strains were isolated from bovine mastitis milk samples from a Chinese dairy farm, and they were identified as H. ovis by microscopic examination and 16S rRNA gene sequencing. Phylogenetic tree was constructed using 16S rRNA gene, and the isolates were closely related to other China strains and strains from Japan. The growth speed of the H. ovis isolated was relatively slower than Streptococcus agalactiae, and the phenotypic characteristics were similar to H. ovis CCUG37441 and CCUG39041 except to lactose. The isolates were sensitive to most of the common used antimicrobials. The H. ovis isolates could lead to mild murine mastitis alone and induce severe mastitis when co-infected with Trueperella pyogenes in the murine mammary infection model constructed.
Canine Melanoma Immunology and Immunotherapy: Relevance of Translational Research
Authors: Tarone Lidia , Giacobino Davide , Camerino Mariateresa , Ferrone Soldano , Buracco Paolo , Cavallo Federica , Riccardo Federica
Published: February 2022

In veterinary oncology, canine melanoma is still a fatal disease for which innovative and long-lasting curative treatments are urgently required. Considering the similarities between canine and human melanoma and the clinical revolution that immunotherapy has instigated in the treatment of human melanoma patients, special attention must be paid to advancements in tumor immunology research in the veterinary field. Herein, we aim to discuss the most relevant knowledge on the immune landscape of canine melanoma and the most promising immunotherapeutic approaches under investigation. Particular attention will be dedicated to anti-cancer vaccination, and, especially, to the encouraging clinical results that we have obtained with DNA vaccines directed against chondroitin sulfate proteoglycan 4 (CSPG4), which is an appealing tumor-associated antigen with a key oncogenic role in both canine and human melanoma. In parallel with advances in therapeutic options, progress in the identification of easily accessible biomarkers to improve the diagnosis and the prognosis of melanoma should be sought, with circulating small extracellular vesicles emerging as strategically relevant players. Translational advances in melanoma management, whether achieved in the human or veterinary fields, may drive improvements with mutual clinical benefits for both human and canine patients; this is where the strength of comparative oncology lies.
Robust Registration of Medical Images in the Presence of Spatially-Varying Noise
Authors: Abbasi-Asl Reza , Ghaffari Aboozar , Fatemizadeh Emad
Published: February 2022

Spatially-varying intensity noise is a common source of distortion in medical images and is often associated with reduced accuracy in medical image registration. In this paper, we propose two multi-resolution image registration algorithms based on Empirical Mode Decomposition (EMD) that are robust against additive spatially-varying noise. EMD is a multi-resolution tool that decomposes a signal into several principle patterns and residual components. Our first proposed algorithm (LR-EMD) is based on the registration of EMD feature maps from both floating and reference images in various resolutions. In the second algorithm (AFR-EMD), we first extract a single average feature map based on EMD and then use a simple hierarchical multi-resolution algorithm to register the average feature maps. We then showcase the superior performance of both algorithms in the registration of brain MRIs as well as retina images. For the registration of brain MR images, using mutual information as the similarity measure, both AFR-EMD and LR-EMD achieve a lower error rate in intensity (42% and 32%, respectively) and lower error rate in transformation (52% and 41%, respectively) compared to intensity-based hierarchical registration. Our results suggest that the two proposed algorithms offer robust registration solutions in the presence of spatially-varying noise.
Low Speed Aerodynamic Analysis of the N2A Hybrid Wing–Body
Authors: Aprovitola Andrea , Aurisicchio Francesco , Di Nuzzo Pasquale Emanuele , Pezzella Giuseppe , Viviani Antonio
Published: February 2022

Reduction of atmospheric emissions is currently a mandatory requirement for aircraft manufacturers. Several studies performed on Blended Wing–Body configurations showed a promising capability of reducing fuel consumption by increasing, at the same time, passengers’ transport capabilities. Although several aerodynamic studies are available at transonic speeds, low-speed evaluations of aerodynamic performances of Blended Wing Body aircrafts are less investigated. In this framework, the present paper deals with the aerodynamic performance of the N2A aircraft prototype at low-Mach number conditions. Aircraft longitudinal aerodynamics is addressed at M∞=0.2 with steady state three-dimensional RANS simulations carried out at two Reynolds numbers equal to 6.60×106 and 1.27×108, respectively. The former refers to an experimental test campaign performed at NASA Langley 14-by-22 foot subsonic tunnel, while the latter is related to free-flight conditions close to an approach and landing phase. Flowfield simulations are performed using the Computational Fluid Dynamic code FLUENT and the SU2 open-source code, currently adopted for research applications. Numerical solutions are validated by using available experimental data with reference to lift, drag, pitching moment and drag polar estimations. Pre-stall and post-stall aerodynamic behaviour through mean flow-field visualization along with the comparison of pressure distributions at several AoAs is addressed. Furthermore, the effect of convective discretization on a numerical solution for SU2 is discussed. Results indicate a good agreement with available experimental predictions. The present study aims to bridge existing computations at a Eulerian low-Mach number, with RANS computations and constitutes a further test-case for SU2 code with respect to a full aircraft configuration.
Characterization of Residual Stresses and Grain Structure in Hot Forging of GH4169
Authors: Wang Zibiao , Hou Guangsha , Zhao Yang , Sun Jianfei , Guo Jiangzhen , Chen Wuyi
Published: February 2022

Residual stresses (RS) in hot forging severely degrade the machining accuracy and stability of super alloy parts. This is the main reason for deformation during subsequent mechanical machining. RS need recognition, as well as the microstructure and properties achieved by forging. In this study, a simulation and experimental research on the single-pass compression of GH4169 are presented. RS variations with forging temperature, loading speed, and cooling speed are established by finite element (FE) simulation. Based on the FE results, an experiment is conducted at a temperature of 1020 ℃, loading speed of 25 mm/s, and press amount of 16 mm, immediately followed by water cooling. A new layer-stripping method is put forward for the high-efficiency measurement and correction of interior RS. Compared with the traditional strain gauge layer-stripping method, the measurement efficiency of the new layer-stripping method is increased by 10 times. Meanwhile, grain photographs are collected and grain size evolution is summarized; thus, the RS is characterized and evaluated from the angle of grains. It is demonstrated that the RS level rises with the increase in forging temperature, loading speed, and cooling speed, while the cooling method influences both the stress value and distribution. Compressive RS changes to tensile, while the average grain size reduces from the surfaces to the center. In the compressive regions, stress values share the same rules as grain size, while, in the tensile regions, they are contrary. The RS levels are divided according to the grain degree standard. According to the residual stress and grain distribution law of the blank, the optimal position of the part in the blank can be determined. Compared with the center position of the part in the blank, the residual stress of the part is reduced by 70%. The results provide useful strategies for the better design of forging technology, qualification examinations, and subsequent mechanical machining.
RNN-CNN Hybrid Model to Predict C-ATC CAPACITY Regulations for En-Route Traffic
Authors: Mas-Pujol Sergi , Salamí Esther , Pastor Enric
Published: February 2022

Meeting the demand with the available airspace capacity is one of the most challenging problems faced by Air Traffic Management. Nowadays, this collaborative Demand–Capacity Balancing process often ends up enforcing Air Traffic Flow Management regulations when capacity cannot be adjusted. This process to decide if a regulation is needed is time consuming and relies heavily on human knowledge. This article studies three different Air Traffic Management frameworks aiming to improve the cost-efficiency for Flow Manager Positions and Network Manager operators when facing the detection of regulations. For this purpose, two already tested Deep Learning models are combined, creating different hybrid models. A Recurrent Neural Network is used to process scalar variables to extract the overall airspace characteristics, and a Convolutional Neural Network is used to process artificial images exhibiting the specific airspace configuration. The models are validated using historical data from two of the most regulated European regions, resulting in a novel framework that could be used across Air Traffic Control centers. For the best hybrid model, using a cascade architecture, an average accuracy of 88.45% is obtained, with an average recall of 92.16%, and an average precision of 86.85%, across different traffic volumes. Moreover, two different techniques for model explainability are used to provide a theoretical understanding of its behavior and understand the reasons behind the predictions.
Simultaneous Detection of Plant- and Fungus-Derived Genes Constitutively Expressed in Single Pseudoidium neolycopersici-Inoculated Type I Trichome Cells of Tomato Leaves via Multiplex RT-PCR and Nested PCR
Authors: Iwasaki Shota , Okada Naoko , Kimura Yutaka , Takikawa Yoshihiro , Suzuki Tomoko , Kakutani Koji , Matsuda Yoshinori , Bai Yuling , Nonomura Teruo
Published: February 2022

Type I trichomes of tomato leaves (Solanum lycopersicum Mill. cv. Moneymaker), as outgrowths of the plant epidermis, are suitable for monitoring infection processes of powdery mildew species using a high-fidelity digital microscope (DM) without fungal staining. On the trichomes, tomato powdery mildew (Pseudoidium neolycopersici L. Kiss) isolate KTP-03 produced a maximum of four vigorously elongated hyphae per conidium, which stopped growth approximately 12 days after inoculation. Single trichome cells, invaded by fungal hyphae at various fungal infection stages during the 12-day period after the inoculation of single conidia, were cut at the bases and directly collected with small precision scissors (i.e., microscissors) held by the manipulator under a DM. Subsequently, suc-polymerase chain reaction (PCR) (reverse transcription (RT)-PCR followed by nested (N)-PCR) was conducted to explore gene expression in the infected trichome. We selected intron-containing genes from tomatoes and powdery mildew fungi for the detection of constitutive gene transcripts, namely plasma membrane H+-ATPase (LHA2) and β-tubulin 2 (TUB2) genes. In suc-PCR, a single band from spliced mRNAs of both LHA2 and TUB2 genes were detected, suggesting that both genes were successfully transcribed in single KTP-03-infected trichomes. With combined primers for both LHA2 and TUB2 (multiplex RT-PCR/N-PCR), two bands were detected through the amplification of intron-spliced mRNAs of both genes. Therefore, our single-trichome cell PCR amplification method is effective for detecting the expression patterns of genes from both tomato and powdery mildew fungus. Combinations of digital microscopy, microscissors, and multiplex RT-PCR/N-PCR amplification techniques will be useful for simultaneously analysing the molecular interactions between plants and powdery mildew fungi at the level of single tomato leaf trichome cells. Also, this employed technique will be of benefit in other plant species and crops, possessing leaf trichome cells, to elucidate the molecular interactions between plants and pathogens.
Relationship between Grain Yield and Quality Traits under Optimum and Low-Nitrogen Stress Environments in Tropical Maize
Authors: Ertiro Berhanu Tadesse , Das Biswanath , Kosgei Titus , Tesfaye Amsal Tarekegne , Labuschagne Maryke T. , Worku Mosisa , Olsen Michael S. , Chaikam Vijay , Gowda Manje
Published: February 2022

Breeding for nitrogen use efficiency (NUE) is important to deal with food insecurity and its effect on grain quality, particularly protein. A total of 1679 hybrids were evaluated in 16 different trials for grain yield (GY), grain quality traits (protein, starch and oil content) and kernel weight (KW) under optimum and managed low soil nitrogen fields in Kiboko, Kenya, from 2011 to 2014. The objectives of our study were to understand (i) the effect of low soil N stress on GY and quality traits, (ii) the relationship between GY and quality traits under each soil management condition and (iii) the relationship of traits with low-N versus optimum conditions. From the results, we observed the negative effects of low N on GY, KW and the percentage of protein content, and a positive effect on the percentage of starch content. The correlation between GY and all quality traits was very weak under both soil N conditions. GY had a strong relationship with KW under both optimum and low soil N conditions. Protein and starch content was significantly negative under both optimum and low-N conditions. There was no clear relationship among quality traits under optimum and low N, except for oil content. Therefore, it seems feasible to simultaneously improve GY along with quality traits under both optimum and low-N conditions, except for oil content. However, the negative trend observed between GY (starch) and protein content suggests the need for the regular monitoring of protein and starch content to identify varieties that combine both high GY and acceptable quality. Finally, we recommend further research with a few tropical maize genotypes contrasting for NUE to understand the relationship between the change in grain quality and NUE under low-N conditions.
Culling Double Counting in Sequence Images for Fruit Yield Estimation
Authors: Xia Xue , Chai Xiujuan , Zhang Ning , Zhang Zhao , Sun Qixin , Sun Tan
Published: February 2022

Exact yield estimation of fruits on plants guaranteed fine and timely decisions on harvesting and marketing practices. Automatic yield estimation based on unmanned agriculture offers a viable solution for large orchards. Recent years have witnessed notable progress in computer vision with deep learning for yield estimation. Yet, the current practice of vision-based yield estimation with successive frames may engender fairly great error because of the double counting of repeat fruits in different images. The goal of this study is to provide a wise framework for fruit yield estimation in sequence images. Specifically, the anchor-free detection architecture (CenterNet) is utilized to detect fruits in sequence images from videos collected in the apple orchard and orange orchard. In order to avoid double counts of a single fruit between different images in an image sequence, the patch matching model is designed with the Kuhn–Munkres algorithm to optimize the paring process of repeat fruits in a one-to-one assignment manner for the sound performance of fruit yield estimation. Experimental results show that the CenterNet model can successfully detect fruits, including apples and oranges, in sequence images and achieved a mean Average Precision (mAP) of 0.939 under an IoU of 0.5. The designed patch matching model obtained an F1-Score of 0.816 and 0.864 for both apples and oranges with good accuracy, precision, and recall, which outperforms the performance of the reference method. The proposed pipeline for the fruit yield estimation in the test image sequences agreed well with the ground truth, resulting in a squared correlation coefficient of R2apple = 0.9737 and R2orange = 0.9562, with a low Root Mean Square Error (RMSE) for these two varieties of fruit.
Efficacy of Selected Insecticides for Chemical Control of the African Citrus Psyllid, Trioza erytreae (Psylloidea: Triozidae)
Authors: Molina Paula , Hernández-Suárez Estrella , Rizza Rositta , Martínez-Ferrer María Teresa , Campos-Rivela José Miguel , Agustí Nuria , Siverio Felipe , Hervalejo Aurea , Arenas-Arenas Francisco José
Published: February 2022

The recent spread of the African citrus psyllid, Trioza erytreae, one of the vectors of the devastating citrus disease, Huanglongbing (HLB), to parts of mainland Europe has created considerable concern. In this study, we show the efficacy of several insecticides with varying modes of action on different developmental stages of T. erytreae. In laboratory trials, spinetoram caused the highest mortality in T. erytreae eggs (between 80 and 90%), while dimethoate, lambda cyhalothrin, spinetoram, cyantraniliprole, and paraffin oil showed over 90% mortality on nymphs. Dimethoate, spinetoram and paraffin oil also demonstrated high efficacy against adults. In winter field conditions, dimethoate showed the best results to control T. erytreae nymph populations, and lambda cyhalothrin showed persistent egg control. Our results support the use of different insecticides to control T. erytreae for adults in winter, and for egg and nymph populations in spring and summer.
The Role of Social Interaction and Personal Characteristics in Affecting the Adoption of Compost from Organic Fraction of Municipal Solid Waste in Italy
Authors: Bagagiolo Giorgia , Vigoroso Lucia , Pampuro Niccolò , Cavallo Eugenio
Published: February 2022

The composting process allows one to minimize quantities of the organic fraction of municipal solid waste (OFMSW) disposed through landfills. Compost obtained from OFMSW is not yet widespread across all European countries, including Italy. Even though previous studies emphasized the role that social interaction and socio-demographic users’ characteristics may have toward the adoption of sustainable practices, to our knowledge, few studies have specifically addressed OFMSW compost. Considering the need to integrate the social perspective in sustainable agriculture literature, further research is required regarding the intention to adopt this organic product, especially among potential users. A questionnaire was used to investigate potential users’ level of interest in using OFMSW compost and their a priori perceptions on its fertilizing properties, if anyone ever suggested using OFMSW compost, as well as the participants’ socio-demographic characteristics. Fifty-eight participants were involved. The results demonstrated a significant association between education level and received suggestions from peer or social networks in the interest of adopting OFMSW compost. In addition, participants who received suggestions had higher odds of being highly interested in adopting OFMSW compost versus those who did not receive it. In conclusion, institutions, where formal knowledge has a place, and agricultural policy makers should be engaged with the aim to facilitate knowledge exchange and connections among different actors, supporting local-level initiatives.
A High-Throughput Imagery Protocol to Predict Functionality upon Fractality of Carbon-Capturing Biointerfaces
Authors: Moreira Bruno Rafael de Almeida , Brito Filho Armando Lopes de , Barbosa Júnior Marcelo Rodrigues , Silva Rouverson Pereira da
Published: February 2022

Surface quality is key for any adsorbent to have an effective adsorption. Because analyzing an adsorbent can be costly, we established an imagery protocol to determine adsorption robustly yet simply. To validate our hypothesis of whether stereomicroscopy, superpixel segmentation and fractal theory consist of an exceptional merger for high-throughput predictive analytics, we developed carbon-capturing biointerfaces by pelletizing hydrochars of sugarcane bagasse, pinewood sawdust, peanut pod hull, wheat straw, and peaty compost. The apochromatic stereomicroscopy captured outstanding micrographs of biointerfaces. Hence, it enabled the segmenting algorithm to distinguish between rough and smooth microstructural stresses by chromatic similarity and topological proximity. The box-counting algorithm then adequately determined the fractal dimension of microcracks, merely as a result of processing segments of the image, without any computational unfeasibility. The larger the fractal pattern, the more loss of functional gas-binding sites, namely N and S, and thus the potential sorption significantly decreases from 10.85 to 7.20 mmol CO2 g−1 at sigmoid Gompertz function. Our insights into analyzing fractal carbon-capturing biointerfaces provide forward knowledge of particular relevance to progress in the field’s prominence in bringing high-throughput methods into implementation to study adsorption towards upgrading carbon capture and storage (CCS) and carbon capture and utilization (CCU).
Genetic Diversity of Flavescence Dorée Phytoplasmas in Vineyards of Serbia: From the Widespread Occurrence of Autochthonous Map-M51 to the Emergence of Endemic Map-FD2 (Vectotype II) and New Map-FD3 (Vectotype III) Epidemic Genotypes
Authors: Krstić Oliver , Cvrković Tatjana , Marinković Slavica , Jakovljević Miljana , Mitrović Milana , Toševski Ivo , Jović Jelena
Published: February 2022

Flavescence dorée (FD) is a European quarantine disease of grapevine caused by FD phytoplasma (FDp) transmitted by the leafhopper of North American origin Scaphoideus titanus. The disease affects the most important viticultural regions of Europe and all wine-growing regions of Serbia. Unlike the insect vector, the pathogen is native to Europe and associated with several wild host plants among which alder trees as the main source of two out of three map genetic clusters of pathogen variants (Map-FD1 and FD2). Heretofore, the FDp epidemic in Serbian vineyards was thought to be monotypic, i.e., caused by the single genotype of the Map-FD3 cluster, M51, and correlated with clematis as the natural source plant. This study aimed to provide data on genetic diversity, through map and vmpA gene typing, and insights into ecological properties of epidemiological cycles driving the epidemic outbreaks of FD in Serbia today. Map genotyping of 270 grapevine isolates collected from 2017 to 2019 confirmed M51 as autochthonous genotype widespread in all wine producing regions of Serbia and the dominant FDp epidemic genotype in most of the districts (75%, 202/270 isolates), except in north Serbia where multiple outbreaks of M12 Map-FD3 were recorded (54 isolates). Tree of heaven is reported as a new FDp plant reservoir for the Serbian vineyards, hosting the M51 genotype, along with clematis. An outbreak of a new endemic Map-FD3 genotype M144 was documented in grapevine samples from east Serbia (5 isolates), correlating with previous findings of the same genotype in clematis. In addition, single grapevine infections with five new Map-FD3 genotypes (M150-M154) were recorded in central Serbia, thus indicating high endemic potential for new outbreaks. The vmpA typing placed all Map-FD3 isolates into the VmpA-III cluster, i.e., Vectotype III. Finally, we found direct evidence that at least two FDp endemic genotypes, M89 and M148, of the Map-FD2/VmpA-II have escaped from alders and propagated in the grapevine-S. titanus pathosystem in Serbia (Vectotype II). Our findings confirm the high complexity of the FDp ecological cycle and provide evidence of a unique, autochthonous Balkan epidemiology sourced endemically.
Dead Brood of Apis mellifera Is Removed More Effectively from Small-Cell Combs Than from Standard-Cell Combs
Authors: Dziechciarz Piotr , Borsuk Grzegorz , Olszewski Krzysztof
Published: February 2022

The efficiency of the hygienic behaviour in bee colonies towards dead brood was assessed in small-cell combs (SMCombs) and in standard-cell combs (STCombs). Each colony had both types of combs in the nest on a permanent basis. Simultaneous keeping of a colony on standard- and small-cell combs is a novel approach to the use of small-cell combs in beekeeping. The number of killed pupae removed within 24 h was the measure of the hygienic behaviour efficiency. Regardless of the year, the brood in the SMCombs was uncapped and removed significantly more efficient (p ≤ 0.01) than in the STCombs (number of non-uncapped cells: in 2020 SMCombs = 3.79, STCombs = 11.62; in 2021 SMCombs = 2.34, STCombs = 5.28 and completely removed cells: in 2020 SMCombs = 87.46, STCombs = 80.04; in 2021 SMCombs = 96.75, STCombs = 92.66). In colonies kept simultaneously on standard- and small-cell combs, the width of the comb cells has a significant effect on the efficiency of removal of dead brood, which is removed more efficient from small-cell combs than from standard-cell combs.
Investigating the Behavior and Personality Structure of the Aldabra Tortoise during Human Interactions and Training Events
Authors: Quintavalle Pastorino Giovanni , Smith Vanessa , Faustini Massimo , Bonacina Eleonora , Guadagnini Davide , Robbiati Roberto , Cavalleri Alice , Brereton James Edward , Preziosi Richard
Published: February 2022

Human–animal interaction (HAI) can be valuable for captive animals, and many zoo-housed species benefit from interactions with their keepers. There is also an increasing body of evidence that some animal species possess personalities that are temporally consistent. However, the majority of zoo research, particularly on personality and HAI, traditionally has focused on mammals, and there are comparatively fewer studies on reptiles. Research was undertaken at the Faunistic Park Le Cornelle, Italy, to investigate the effects of approach tests and food interaction events on 5 male and 5 female Aldabra tortoise (Aldrabrachelys gigantea) behavior. During human–tortoise interactions, continuous focal sampling of behavior took place. The behavioral differences between individual tortoises, interaction type (approach or training) and person involved in the interaction (keeper, vet, or observer) were analysed using general linear mixed-effects models. The personality components of the tortoises were also quantified using principal component analysis. Overall, the study revealed that individuals acted significantly differently to one another, and that while females initially appeared to display a greater number of shy behaviors, this was not consistent across the group. PCA revealed two personality dimensions in the tortoises: boldness and avoidance. Overall, the study revealed that the tortoises possessed personalities that were independent of sex or scenario. The study also revealed that many tortoises chose to interact with their keepers during training and approach tests: this suggests that human–tortoise interactions may have some enrichment value. Future research could quantify tortoise personality in other scenarios such as in social interactions with other tortoises.
A Comparative Analysis of Metabolic Profiles of Embryonic Skeletal Muscle from Lantang and Landrace Pigs
Authors: Cai Shufang , Duo Tianqi , Wang Xiaoyu , Tong Xian , Luo Chenglong , Chen Yaosheng , Li Jianhao , Mo Delin
Published: February 2022

Elucidation of the complex regulation of porcine muscle development is key to increasing pork output and improving pork quality. However, the molecular mechanisms involved in early porcine embryonic muscle development in different pig breeds remain largely unknown. Here, GC–MS based metabolomics and metabolomic profiling was used to examine the longissimus lumborum (LL) of the Lantang (LT) and the Landrace (LR) pig at embryonic day 35 (E35). Metabolites showed clear separation between LT and LR, with 40 metabolites having higher abundances in LT and 14 metabolites having lower abundances in LT compared with LR. In addition, these metabolic changes were mainly associated with nucleotide metabolism and energy metabolism, such as purine metabolism, pyrimidine metabolism, the pentose phosphate pathway, and the TCA cycle. More interestingly, the contents of DNA, RNA, and ATP per unit mass of LL tissues were higher in LT, indicating rapid synthesis of nucleic acids and ATP, to meet both the material and energy requirements of rapid cell proliferation and differentiation. Furthermore, enzyme activity associated with the TCA cycle and pentose phosphate pathway, including α-ketoglutaric dehydrogenase (KGDH), malate dehydrogenase (MDH), pyruvate dehydrogenase (PDH), succinate dehydrogenase (SDH), and glucose-6-phosphate dehydrogenase (G6PDH), were higher in LT. Based on these results, we conclude that there are significant differences in nucleotide metabolism and energy metabolism of LL between LT and LR, and we speculate that the enhanced nucleic acid metabolism and energy metabolism in LT can meet the material and energy requirements of rapid cell proliferation and differentiation, making myogenesis more intense in LT compared to LR which might be the metabolic mechanism underlying the distinct skeletal muscle development in the two breeds.
Aggression, Erection, and Masturbation in Feral Pottoka Ponies and Implications for Equine Welfare
Authors: Grillaert Katherine
Published: February 2022

Erection and masturbation in horses are considered unwanted behaviors in training contexts, despite recognition that these are naturally occurring behaviors that are integral to the welfare of male horses. Equestrians, especially those who use positive reinforcement in their training, expressed concern that the presence of such behaviors might be associated with aggressive or sexual behaviors aimed at humans participating in horse-human interactions. The implications of such attitudes could negatively affect male horses by excluding them from welfare-friendly training systems. In this study, feral stallions were observed to describe and quantify behaviors that occurred before, during, and after erection and masturbation, and to identify change in arousal. This study did not find evidence that erection and masturbation are associated with increases in arousal, or to sexual or aggressive behavior toward other horses. The possible presence or induction of erection or masturbation alone might not limit male horses from participating in certain handling, training, or riding contexts. These findings, along with further research, may be used to inform interpretations of horse–human interactions that involve erection or masturbation.
Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture—A Practical Review
Authors: Ryu Jun Hyung , Xu Lan , Wong Ten-Tsao
Published: February 2022

Germ cell transplantation technology enables surrogate offspring production in fish. This technology has been expected to mitigate reproductive barriers, such as long generation time, limited fecundity, and complex broodstock management, enhancing seed production and productivity in aquaculture. Many studies of germ cell transplantation in various fish species have been reported over a few decades. So far, surrogate offspring production has been achieved in many commercial species. In addition, the knowledge of fish germ cell biology and the related technologies that can enhance transplantation efficiency and productivity has been developed. Nevertheless, the commercial application of this technology still seems to lag behind, indicating that the established models are neither beneficial nor cost-effective enough to attract potential commercial users of this technology. Furthermore, there are existing bottlenecks in practical aspects such as impractical shortening of generation time, shortage of donor cells with limited resources, low efficiency, and unsuccessful surrogate offspring production in some fish species. These obstacles need to be overcome through further technology developments. Thus, we thoroughly reviewed the studies on fish germ cell transplantation reported to date, focusing on the practicality, and proposed potential solutions and future perspectives.
The Immunological Role of Vascular and Lymphatic Endothelial Cells in Filarial Infections
Authors: Wysmołek Magdalena Elżbieta , Długosz Ewa , Wiśniewski Marcin
Published: February 2022

The embryonic stage of filarial nematodes, or microfilariae (Mf), shows daily and seasonal periodicity that requires their migration through blood vessels into the lungs, where they are sequestered when not circulating in the peripheral blood. Therefore, Mf and the host endothelium are likely in a permanent state of hide and seek. Interestingly, filarial nematodes co-cultured in media with a murine endothelial cell line survive eight times longer than those cultured in media alone. This suggests that the endothelium is an important element of the immune response in filarial nematodes, perversely promoting their survival in the host. In this review, we will focus on potential pathways involved in the relationship between filarial nematodes and the host endothelium, including the role of endothelial ICAM/VCAM/PECAM adhesion molecules, surface markers involved in the passage of Mf through host tissue, anti-thrombolic effects caused by the presence of filarial nematodes (including plasmins), endothelial cell proliferation (VEGF), and other aspects of the immune activation of the endothelium. The aim of this review is to merge the knowledge about the cross-talk between Mf of different filarial nematode species and endothelial cells (EC), thus allowing a better understanding of the mechanism of these parasitic infections.
Effects of Increasing Levels of Palm Kernel Oil in the Feed of Finishing Lambs
Authors: Castro Daniela Pionorio Vilaronga , Pimentel Paulo Roberto Silveira , da Silva Júnior Jarbas Miguel , Virgínio Júnior Gercino Ferreira , de Andrade Ederson Américo , Barbosa Analívia Martins , Pereira Elzânia Sales , Ribeiro Claudio Vaz Di Mambro , Bezerra Leilson Rocha , Oliveira Ronaldo Lopes
Published: February 2022

The aim of this study was to evaluate the effects of the inclusion of palm kernel oil (PKO) in a lamb diet on nutrient intake, digestibility, ingestive behavior, nitrogen balance, blood metabolites, rumen fermentation parameters, and animal performance. Three experimental trials were conducted. The treatments consisted of varying levels of PKO included in the diet, with PKOzero = no PKO inclusion, PKO1.3 = 1.3% addition, PKO2.6 = 2.6% addition, PKO3.9 = 3.9% addition, and PKO5.2 = 5.2% addition, based on the total dry matter (DM) of the diet. With the inclusion of PKO in the diet, linear decreases in DM (p < 0.001), crude ash (p < 0.001), crude protein (CP) (p < 0.001), neutral detergent fiber (NDF) (p < 0.001), nonfibrous carbohydrate (NFC) (p < 0.001), and total digestible nutrient (TDN) (p = 0.021) intake were observed, as was an increase in ether extract (EE) intake (p < 0.001). The digestibility coefficients of NDF and NFC were not affected by PKO addition to the diet. However, the digestibility of DM (p = 0.035), EE (p < 0.001), CP (p < 0.001), and TDNs (p < 0.001) increased when PKO was added to the lambs’ diet. Reductions in N intake (p < 0.001), fecal nitrogen excretion (p < 0.001), and microbial protein production (p < 0.001) were noted with increasing PKO levels. Serum cholesterol increased (p < 0.001) while serum GGT enzyme concentrations in the blood decreased (p = 0.048) with increasing PKO levels. PKO addition had no effect on total weight gain and average daily gain; however, feed conversion improved (p = 0.001) with increasing PKO levels. The intake, digestibility, ingestive behavior, and growth performance of lambs with PKO1.3 added to their diet were similar to animals that did not receive PKO, meaning that PKO could be an alternative energy source for growing lambs because it does not harm animal performance and can lower the cost of feed.
New Antibacterial Secondary Metabolites from a Marine-Derived Talaromyces sp. Strain BTBU20213036
Authors: Song Fuhang , Dong Yifei , Wei Shangzhu , Zhang Xinwan , Zhang Kai , Xu Xiuli
Published: February 2022

New polyketide-derived oligophenalenone dimers, bacillisporins K and L (1 and 2) and xanthoradone dimer rugulosin D (3), together with four known compounds, bacillisporin B (4), macrosporusone D (5), rugulosin A and penicillide (6 and 7), were isolated from the marine-derived fungus Talaromyces sp. BTBU20213036. Their structures were determined by detailed analysis of HRESIMS, 1D and 2D NMR data, and the absolute configurations were determined on the basis of calculated and experimental electronic circular dichroism (ECD). The antibacterial and antifungal activities of these compounds were tested against Gram-positive—Staphylococcus aureus, Gram-negative—Escherichia coli, and fungal strain—Candida albicans. These compounds showed potential inhibitory effects against S. aureus with minimum inhibitory concentrations ranging from 0.195 to 100 µg/mL.
Canadian Collaboration to Identify a Minimum Dataset for Antimicrobial Use Surveillance for Policy and Intervention Development across Food Animal Sectors
Authors: Léger David F. , Anderson Maureen E. C. , Bédard François D. , Burns Theresa , Carson Carolee A. , Deckert Anne E. , Gow Sheryl P. , James Cheryl , Li Xian-Zhi , Ott Michael , Agunos Agnes
Published: February 2022

Surveillance of antimicrobial use (AMU) and antimicrobial resistance (AMR) is a core component of the 2017 Pan-Canadian Framework for Action. There are existing AMU and AMR surveillance systems in Canada, but some stakeholders are interested in developing their own AMU monitoring/surveillance systems. It was recognized that the establishment of core (minimum) AMU data elements, as is necessary for policy or intervention development, would inform the development of practical and sustainable AMU surveillance capacity across food animal sectors in Canada. The Canadian Animal Health Surveillance System (CAHSS) AMU Network was established as a multisectoral working group to explore the possibility of harmonizing data inputs and outputs. There was a consensus that a minimum AMU dataset for AMU surveillance (MDS-AMU-surv) should be developed to guide interested parties in initiating AMU data collection. This multisectoral collaboration is an example of how consultative consensus building across relevant sectors can contribute to the development of harmonized approaches to AMU data collection and reporting and ultimately improve AMU stewardship. The MDS-AMU-surv could be used as a starting point for the progressive development or strengthening of AMU surveillance programs, and the collaborative work could serve as a model for addressing AMR and other shared threats at the human–animal–environment interface.
Could Dietary Supplementation with Different Sources of N-3 Polyunsaturated Fatty Acids Modify the Rabbit Gut Microbiota?
Authors: Curone Giulio , Biscarini Filippo , Cotozzolo Elisa , Menchetti Laura , Dal Bosco Alessandro , Riva Federica , Cremonesi Paola , Agradi Stella , Mattioli Simona , Castiglioni Bianca , Di Giancamillo Alessia , Cartoni Mancinelli Alice , Draghi Susanna , Quattrone Alda , Collodel Giulia , Modina Silvia Clotilde , Castellini Cesare , Brecchia Gabriele
Published: February 2022

The present study evaluated the effects of feed supplemented with two dietary sources of n-3 polyunsaturated fatty acids (PUFAs; fish oil and extruded flaxseed) on the gut microbiota, caecal fermentations, gastrointestinal histology, and histochemistry in rabbits. Fifteen male New Zealand White rabbits were divided into three groups (n = 5/group) and fed with different diets from weaning (35 days of age) until slaughtering (90 days of age): C group, fed with a commercial diet; F group, supplemented with 10% of extruded flaxseed; and O group, supplemented with 3.5% of fish oil. At slaughter, the content of the stomach, duodenum, jejunum, ileum, caecum, and colon was collected and analyzed by Next Generation 16S rRNA gene sequencing. Tissue samples of the same tracts were evaluated with histological and histochemical analysis. Ammonia and lactic acid in the caecum were also quantified. Twenty-nine operational taxonomic units (OTUs) were significantly different between groups. Groups receiving n-3 PUFAs supplementation showed an increase in Bacteroidetes and Lachnospiraceae in several gastrointestinal tracts, while Bacilli abundance, as well as Firmicutes/Bacteroidetes ratio, were reduced compared to the control group (for all p < 0.05). Caecal ammonia was lower in the F than C group (p < 0.032), whereas no difference was found for lactic acid. Finally, histological evaluations revealed a mild hemorrhagic infiltration and vessels ectasia in the stomach mucosa of both F and O groups, but no effect of nutritional treatment was evidenced by the histochemical analyses. In conclusion, n-3 PUFAs supplementation could modify the rabbit gut microbiota and fermentation. The increase in beneficial bacterial populations may, at least partially, explain the positive effects of n-3 PUFAs diet supplementation on human and animals’ health, although the appropriate dosage should be established.