bluuebunny/crossref_metadata_2025 huggingface dataset:
A Study of Wave Dissipation Rate and Particles Velocity in Muddy Beds
Authors: Shamsnia S. Hadi , Soltanpour Mohsen , Bavandpour Majid , Gualtieri Carlo
Published: May 2019

The interactions between free surface waves and layers of cohesive sediments including wave height attenuation and mud movement are of great importance in coastal and marine engineering. In this study, the results from a new analytical model were compared with those from literature experimental works and analytical models in terms of wave height dissipation rate and mud velocity. It was found that the new model provided good agreements in the case of coexisting waves and currents, while the literature model of Ng (explained in Section 2 of the text) —assuming the mud layer as a highly viscous layer with high shear rates—matched well with the experimental data for high viscosity (mud viscosity, νm = O [0.01 m2/s]). In addition, it was found that the new model is able to successfully simulate particles velocity in the presence of co-current.
Automatic Detection of Trawl-Marks in Sidescan Sonar Images through Spatial Domain Filtering, Employing Haar-Like Features and Morphological Operations
Authors: Gournia Charikleia , Fakiris Elias , Geraga Maria , Williams David P. , Papatheodorou George
Published: May 2019

Bottom trawl footprints are a prominent environmental impact of deep-sea fishery that was revealed through the evolution of underwater remote sensing technologies. Image processing techniques have been widely applied in acoustic remote sensing, but accurate trawl-mark (TM) detection is underdeveloped. The paper presents a new algorithm for the automatic detection and spatial quantification of TMs that is implemented on sidescan sonar (SSS) images of a fishing ground from the Gulf of Patras in the Eastern Mediterranean Sea. This method inspects any structure of the local seafloor in an environmentally adaptive procedure, in order to overcome the predicament of analyzing noisy and complex SSS images of the seafloor. The initial preprocessing stage deals with radiometric inconsistencies. Then, multiplex filters in the spatial domain are performed with multiscale rotated Haar-like features through integral images that locate the TM-like forms and additionally discriminate the textural characteristics of the seafloor. The final TMs are selected according to their geometric and background environment features, and the algorithm successfully produces a set of trawling-ground quantification values that could be established as a baseline measure for the status assessment of a fishing ground.
Automated Stone Detection on Side-Scan Sonar Mosaics Using Haar-Like Features
Authors: Michaelis Rune , Hass H. Christian , Papenmeier Svenja , Wiltshire Karen H.
Published: May 2019

Stony grounds form important habitats in the marine environment, especially for sessile benthic organisms. For the purpose of habitat demarcation and monitoring, knowledge of the position and abundance of individual stones is necessary. This is especially the case in areas with a scattered occurrence of stones in an environment which is otherwise characterized by relatively mobile sandy sediments. Exposed stones can be detected using side-scan sonar (SSS) data. However, apart from laborious manual identification, there is as yet no automated or semi-automated method available for a fast and spatially resolved detection of stones. In this study, a Haar-like feature detector was trained to identify individual stones on an SSS mosaic (~12 km2) showing heterogeneous sediment distribution. The results of this method were compared with those of manually derived stones. Our study shows that the Haar-like feature detector was able to detect up to 62% of the overall occurrence of stones within the study area. Even though the sheer number of correctly identified stones was influenced by, e.g., the type of sediments and the number of grey values of the mosaic, Haar-like feature detectors provide a relatively easy and fast method to identify stones on SSS mosaics when compared to the manual investigation.
The Association between Substance Abuse and Sexual Misconduct among Macau Youths
Authors: Lo T. Wing , Tse John W. L. , Cheng Christopher H. K. , Chan Gloria H. Y.
Published: May 2019

This study investigates how peer influence, school attachment, and substance abuse are related to sexual behavior, with particular interest in exploring the relationship between substance abuse and sexual misconduct, while using a stratified random sample of adolescents in Macau. Mediation analyses were employed. The results show that substance abuse, apart from susceptibility to peer influence and school attachment/commitment, was significantly related to sexual misconduct. Substance abuse was the best predictor of sexual misconduct, and it significantly mediated the relationship between susceptibility to peer influence, as well as school attachment and sexual misconduct. This reflects that the use of substances, including drugs, alcohol, and cigarettes, can be viewed as a catalyst for triggering engagement in sexual misconduct. The implications of this study involve taking measures to reduce the rate of substance abuse as a way of decreasing sexual misconduct in adolescents. Future research directions in exploring the relationship between adolescent substance abuse and risky sexual behavior are discussed.
Primary Drivers of Willingness to Continue to Participate in Community-Based Health Screening for Chronic Diseases
Authors: Chien Shih-Ying , Chuang Ming-Chuen , Chen I-Ping , Yu Peter H.
Published: May 2019

Background: As the average age of the population continues to rise in the 21st century, chronic illnesses have become the most prominent threats to human health. Research has shown that early screenings for chronic diseases are an effective way of lowering incidence and mortality rates. However, low participation rates for health screening is one of the main challenges for preventive medicine. The objective of this study was to determine the primary drivers which: (1) first motivate people to participate in community-based health screening for chronic diseases; and (2) increase their willingness to continue to participate. Methods: A total of 440 individuals between 30 and 75 years of age were invited to undergo a health screening and then complete an interview questionnaire. Screenings and interviews were conducted in four regions in northern Taiwan. The questionnaire was separated into three sections, which explored sociodemographic differences, drivers of willingness to participate, and willingness to continue to participate respectively. Raw data was analyzed using the statistical software package SPSS (SPSS Inc., Chicago, IL, USA). Main Outcome Measures: Effects of sociodemographic factors on health screening participation rate, drivers of willingness to participate, and willingness to continue to participate. Results: Seventy-three percent of participants responded that they would be willing to continue to join in future health screenings. Notably, elderly people and married people were respectively more likely to participate in preventive health screening than were younger people and people who were single, divorced, separated, or widowed. Level of education was another key driver of willingness to participate in health screening for chronic diseases, as were the concern of relatives/friends and the provision of participation incentives. Discussion: Some of our findings, such as the key drivers of willingness to continue to participate in health screening that we identified, were different from findings of many previous studies conducted in other countries. The current study also found that a higher percentage of participants would be willing to join a similar health screening in the future if the service design is considered in advance and is well-implemented.
Hyperbilirubinemia in Gunn Rats Is Associated with Decreased Inflammatory Response in LPS-Mediated Systemic Inflammation
Authors: Valaskova Petra , Dvorak Ales , Lenicek Martin , Zizalova Katerina , Kutinova-Canova Nikolina , Zelenka Jaroslav , Cahova Monika , Vitek Libor , Muchova Lucie
Published: May 2019

Decreased inflammatory status has been reported in subjects with mild unconjugated hyperbilirubinemia. However, mechanisms of the anti-inflammatory actions of bilirubin (BR) are not fully understood. The aim of this study is to assess the role of BR in systemic inflammation using hyperbilirubinemic Gunn rats as well as their normobilirubinemic littermates and further in primary hepatocytes. The rats were treated with lipopolysaccharide (LPS, 6 mg/kg intraperitoneally) for 12 h, their blood and liver were collected for analyses of inflammatory and hepatic injury markers. Primary hepatocytes were treated with BR and TNF-α. LPS-treated Gunn rats had a significantly decreased inflammatory response, as evidenced by the anti-inflammatory profile of white blood cell subsets, and lower hepatic and systemic expressions of IL-6, TNF-α, IL-1β, and IL-10. Hepatic mRNA expression of LPS-binding protein was upregulated in Gunn rats before and after LPS treatment. In addition, liver injury markers were lower in Gunn rats as compared to in LPS-treated controls. The exposure of primary hepatocytes to TNF-α with BR led to a milder decrease in phosphorylation of the NF-κB p65 subunit compared to in cells without BR. In conclusion, hyperbilirubinemia in Gunn rats is associated with an attenuated systemic inflammatory response and decreased liver damage upon exposure to LPS.
Influence of a Novel Surface of Bioactive Implants on Osseointegration: A Comparative and Histomorfometric Correlation and Implant Stability Study in Minipigs
Authors: Romero-Ruiz Manuel M. , Gil-Mur Francisco Javier , Ríos-Santos José Vicente , Lázaro-Calvo Pedro , Ríos-Carrasco Blanca , Herrero-Climent Mariano
Published: May 2019

Purpose: The objective of this study was to assess the influence of a novel surface of dental implants (ContacTi®) on the osseointegration process in a minipig model. The surface was compared with other existing surfaces on the market (SLA® and SLActive®) by employing bone implant contact analysis (BIC) and implant stability. Method: Twelve minipigs were used with prior authorisation from an ethics committee. Three types of surfaces were tested: SLA® (sand-blasted acid-etched titanium), SLActive® (same but hydrophilic, performed under a nitrogen atmosphere), and ContacTi® (alumina particle bombardment of titanium, bioactivated when treated thermochemically) in 4.1 mm × 8 mm implants with internal connection and a polished neck. Twelve implants of each surface type (N = 36) were placed, sacrificing 1/3 of the animals at 2 weeks of placement, 1/3 at 4 weeks and the remaining 1/3 at 8 weeks. Numerical variables were compared with Analysis of Variance, and the correlation between ISQ and BIC was established with the Spearman’s rank correlation coefficient. Results: SLActive® and ContacTi® surfaces showed elevated osteoconductivity at 4 weeks, maintaining a similar evolution at 8 weeks (large amount of mature lamellar tissue with high maturity and bone quality). The SLA® surface showed slower maturation. The ISQ values in surgery were elevated (above 65), higher at necropsy and higher at 4 and 8 weeks in the SLA® group than in the other two (SLActive® and ContacTi®). No significant correlation was found between ISQ and BIC for each implant surface and necropsy time. Conclusion: The three surfaces analysed showed high RFA and BIC values, which were more favourable for the SLActive® and ContacTi® surfaces. No statistical correlation was found between the RFA and BIC values in any of the three surfaces analysed.
Single-Cell RNA-Sequencing Identifies Activation of TP53 and STAT1 Pathways in Human T Lymphocyte Subpopulations in Response to Ex Vivo Radiation Exposure
Authors: Moreno-Villanueva Maria , Zhang Ye , Feiveson Alan , Mistretta Brandon , Pan Yinghong , Chatterjee Sujash , Wu Winston , Clanton Ryan , Nelman-Gonzalez Mayra , Krieger Stephanie , Gunaratne Preethi , Crucian Brian , Wu Honglu
Published: May 2019

Detrimental health consequences from exposure to space radiation are a major concern for long-duration human exploration missions to the Moon or Mars. Cellular responses to radiation are expected to be heterogeneous for space radiation exposure, where only high-energy protons and other particles traverse a fraction of the cells. Therefore, assessing DNA damage and DNA damage response in individual cells is crucial in understanding the mechanisms by which cells respond to different particle types and energies in space. In this project, we identified a cell-specific signature for radiation response by using single-cell transcriptomics of human lymphocyte subpopulations. We investigated gene expression in individual human T lymphocytes 3 h after ex vivo exposure to 2-Gy gamma rays while using the single-cell sequencing technique (10X Genomics). In the process, RNA was isolated from ~700 irradiated and ~700 non-irradiated control cells, and then sequenced with ~50 k reads/cell. RNA in each of the cells was distinctively barcoded prior to extraction to allow for quantification for individual cells. Principal component and clustering analysis of the unique molecular identifier (UMI) counts classified the cells into three groups or sub-types, which correspond to CD4+, naïve, and CD8+/NK cells. Gene expression changes after radiation exposure were evaluated using negative binomial regression. On average, BBC3, PCNA, and other TP53 related genes that are known to respond to radiation in human T cells showed increased activation. While most of the TP53 responsive genes were upregulated in all groups of cells, the expressions of IRF1, STAT1, and BATF were only upregulated in the CD4+ and naïve groups, but were unchanged in the CD8+/NK group, which suggests that the interferon-gamma pathway does not respond to radiation in CD8+/NK cells. Thus, single-cell RNA sequencing technique was useful for simultaneously identifying the expression of a set of genes in individual cells and T lymphocyte subpopulation after gamma radiation exposure. The degree of dependence of UMI counts between pairs of upregulated genes was also evaluated to construct a similarity matrix for cluster analysis. The cluster analysis identified a group of TP53-responsive genes and a group of genes that are involved in the interferon gamma pathway, which demonstrate the potential of this method for identifying previously unknown groups of genes with similar expression patterns.
Fungal Exocellular (1-6)-β-d-glucan: Carboxymethylation, Characterization, and Antioxidant Activity
Authors: Theis Thais Vanessa , Queiroz Santos Vidiany Aparecida , Appelt Patrícia , M. Barbosa-Dekker Aneli , Vetvicka Vaclav , F. H. Dekker Robert , A. Cunha Mário A.
Published: May 2019

Exocellular (1→6)-β-d-glucan (lasiodiplodan) produced by the fungus Lasiodiplodia theobromae MMPI was derivatized by carboxymethylation using different concentrations of a derivatizing agent. Lasiodiplodan was derivatized by carboxymethylation in an attempt to increase its solubility and enhance its biological activities. Carboxymethylglucans with degrees of substitution (DS) of 0.32, 0.47, 0.51, 0.58, and 0.68 were produced and characterized. FTIR analysis showed a band of strong intensity at 1600 cm−1 and an absorption band at 1421 cm−1, resulting from asymmetric and symmetrical stretching vibrations, respectively, of the carboxymethyl group COO- in the carboxymethylated samples. Thermal analysis showed that native lasiodiplodan (LN) and carboxymethylated derivatives (LC) exhibited thermal stability up to 200–210 °C. X-ray diffractometry demonstrated that both native and carboxymethylated lasiodiplodan presented predominantly an amorphous nature. Scanning electron microscopy revealed that carboxymethylation promoted morphological changes in the biopolymer and increased porosity, and alveolar structures were observed along the surface. The introduction of carboxymethyl groups in the macromolecule promoted increased solubility and potentiated the hydroxyl radical-scavenging activity, suggesting a correlation between degree of substitution and antioxidant activity.
Current Understanding of Autophagy in Pregnancy
Authors: Nakashima Akitoshi , Tsuda Sayaka , Kusabiraki Tae , Aoki Aiko , Ushijima Akemi , Shima Tomoko , Cheng Shi-Bin , Sharma Surendra , Saito Shigeru
Published: May 2019

Autophagy is an evolutionarily conserved process in eukaryotes to maintain cellular homeostasis under environmental stress. Intracellular control is exerted to produce energy or maintain intracellular protein quality controls. Autophagy plays an important role in embryogenesis, implantation, and maintenance of pregnancy. This role includes supporting extravillous trophoblasts (EVTs) that invade the decidua (endometrium) until the first third of uterine myometrium and migrate along the lumina of spiral arterioles under hypoxic and low-nutrient conditions in early pregnancy. In addition, autophagy inhibition has been linked to poor placentation—a feature of preeclamptic placentas—in a placenta-specific autophagy knockout mouse model. Studies of autophagy in human placentas have revealed controversial results, especially with regard to preeclampsia and gestational diabetes mellitus (GDM). Without precise estimation of autophagy flux, wrong interpretation would lead to fixed tissues. This paper presents a review of the role of autophagy in pregnancy and elaborates on the interpretation of autophagy in human placental tissues.
Indole-Based Hydrazones Containing A Sulfonamide Moiety as Selective Inhibitors of Tumor-Associated Human Carbonic Anhydrase Isoforms IX and XII
Authors: Demir-Yazıcı Kübra , Bua Silvia , Akgüneş Nurgül Mutlu , Akdemir Atilla , Supuran Claudiu T. , Güzel-Akdemir Özlen
Published: May 2019

Novel sulfonamidoindole-based hydrazones with a 2-(hydrazinocarbonyl)-3-phenyl-1H-indole-5-sulfonamide scaffold were synthesized and tested in enzyme inhibition assays against the tumor-associated carbonic anhydrase isoforms, hCA IX and XII, and the off-targets, hCA I and II. The compounds showed selectivity against hCA IX and XII over hCA I and II. Six compounds showed KI values lower than 10 nM against hCA IX or XII. Molecular modeling studies were performed to suggest binding interactions between the ligand and the hCA active sites.
PD-L1 Expression in Mastocytosis
Authors: Williams Margaret , Lidke Diane S. , Hartmann Karin , George Tracy I.
Published: May 2019

Programmed death 1 (PD-1), when activated by its ligands PD-L1 and PD-L2, suppresses active immune cells in normal immune regulation to limit autoimmunity and, in tumors, as a mechanism of immune evasion. PD-L1 expression has been described as both a prognostic and predictive marker in many solid and hematologic neoplasms, as targeted therapies against the PD-1/PD-L1 interaction have gained clinical importance. PD-L1 expression has been assessed in a few studies on mastocytosis. We review this literature and the need for further investigation of the tumor-immune interaction in mastocytosis.
Glycation of Plant Proteins: Regulatory Roles and Interplay with Sugar Signalling?
Authors: Shumilina Julia , Kusnetsova Alena , Tsarev Alexander , Janse van Rensburg Henry C. , Medvedev Sergei , Demidchik Vadim , Van den Ende Wim , Frolov Andrej
Published: May 2019

Glycation can be defined as an array of non-enzymatic post-translational modifications of proteins formed by their interaction with reducing carbohydrates and carbonyl products of their degradation. Initial steps of this process rely on reducing sugars and result in the formation of early glycation products—Amadori and Heyns compounds via Schiff base intermediates, whereas their oxidative degradation or reactions of proteins with α-dicarbonyl compounds yield a heterogeneous group of advanced glycation end products (AGEs). These compounds accompany thermal processing of protein-containing foods and are known to impact on ageing, pathogenesis of diabetes mellitus and Alzheimer’s disease in mammals. Surprisingly, despite high tissue carbohydrate contents, glycation of plant proteins was addressed only recently and its physiological role in plants is still not understood. Therefore, here we summarize and critically discuss the first steps done in the field of plant protein glycation during the last decade. We consider the main features of plant glycated proteome and discuss them in the context of characteristic metabolic background. Further, we address the possible role of protein glycation in plants and consider its probable contribution to protein degradation, methylglyoxal and sugar signalling, as well as interplay with antioxidant defense.
Periodontal Health and Oral Microbiota in Patients with Rheumatoid Arthritis
Authors: Eriksson Kaja , Fei Guozhong , Lundmark Anna , Benchimol Daniel , Lee Linkiat , Hu Yue O. O. , Kats Anna , Saevarsdottir Saedis , Catrina Anca Irinel , Klinge Björn , Andersson Anders F. , Klareskog Lars , Lundberg Karin , Jansson Leif , Yucel-Lindberg Tülay
Published: May 2019

This study aimed to investigate the periodontal health of patients with established rheumatoid arthritis (RA) in relation to oral microbiota, systemic and oral inflammatory mediators, and RA disease activity. Forty patients underwent full-mouth dental/periodontal and rheumatological examination, including collection of blood, saliva, gingival crevicular fluid (GCF) and subgingival plaque. Composition of plaque and saliva microbiota were analysed using 16S rRNA sequencing and levels of inflammatory mediators by multiplex-immunoassay. The majority of the patients (75%) had moderate or severe periodontitis and the rest had no/mild periodontitis. Anti-citrullinated protein antibody (ACPA) positivity was significantly more frequent in the moderate/severe periodontitis (86%) compared to the no/mild group (50%). No significance between groups was observed for RA disease duration or activity, or type of medication. Levels of sCD30/TNFRSF8, IFN-α2, IL-19, IL-26, MMP-1, gp130/sIL-6Rß, and sTNF-R1 were significantly higher in serum or GCF, and April/TNFSF13 was significantly higher in serum and saliva samples in moderate/severe periodontitis. The microbial composition in plaque also differed significantly between the two groups. In conclusion, the majority of RA patients had moderate/severe periodontitis and that this severe form of the disease was significantly associated with ACPA positivity, an altered subgingival microbial profile, and increased levels of systemic and oral inflammatory mediators.
Effects of Combined Remote Ischemic Pre-and Post-Conditioning on Neurologic Complications in Moyamoya Disease Patients Undergoing Superficial Temporal Artery-Middle Cerebral Artery Anastomosis
Authors: Choi Eun-Su , Lee Yoon-Sook , Park Byeong-Seon , Kim Byung-Gun , Sohn Hye-Min , Jeon Young-Tae
Published: May 2019

Superficial temporal artery-middle cerebral artery (STA-MCA) anastomosis is the most commonly used treatment for Moyamoya disease. During the perioperative period, however, these patients are vulnerable to ischemic injury or hyperperfusion syndrome. This study investigated the ability of combined remote ischemic pre-conditioning (RIPC) and remote ischemic post-conditioning (RIPostC) to reduce the occurrence of major neurologic complications in Moyamoya patients undergoing STA-MCA anastomosis. The 108 patients were randomly assigned to a RIPC with RIPostC group (n = 54) or a control group (n = 54). Patients in the RIPC with RIPostC group were treated with four cycles of 5-min ischemia and 5-min reperfusion before craniotomy and after STA-MCA anastomosis (RIPostC). The incidence of postoperative neurologic complications and the duration of hospital stay were determined. The overall incidence of neurologic complication was significantly higher in the control group than in the RIPC with RIPostC group (13 vs. 3, p = 0.013). The duration of hospital stay was significantly longer in the control group than in the RIPC with RIPostC group (17.8 (11.3) vs. 13.8 (5.9) days, p = 0.023). Combined remote ischemic pre- and post-conditioning can be effective in reducing neurologic complications and the duration of hospitalization in Moyamoya patients undergoing STA-MCA anastomosis.
Control of Invasion by Epithelial-to-Mesenchymal Transition Programs during Metastasis
Authors: Pearson Gray W.
Published: May 2019

Epithelial-to-mesenchymal transition (EMT) programs contribute to the acquisition of invasive properties that are essential for metastasis. It is well established that EMT programs alter cell state and promote invasive behavior. This review discusses how rather than following one specific program, EMT states are diverse in their regulation and invasive properties. Analysis across a spectrum of models using a combination of approaches has revealed how unique features of distinct EMT programs dictate whether tumor cells invade as single cells or collectively as cohesive groups of cells. It has also been shown that the mode of collective invasion is determined by the nature of the EMT, with cells in a trailblazer-type EMT state being capable of initiating collective invasion, whereas cells that have undergone an opportunist-type EMT are dependent on extrinsic factors to invade. In addition to altering cell intrinsic properties, EMT programs can influence invasion through non-cell autonomous mechanisms. Analysis of tumor subpopulations has demonstrated how EMT-induced cells can drive the invasion of sibling epithelial populations through paracrine signaling and remodeling of the microenvironment. Importantly, the variation in invasive properties controlled by EMT programs influences the kinetics and location of metastasis.
Resting Heart Rate Variability Predicts Vulnerability to Pharmacologically-Induced Ventricular Arrhythmias in Male Rats
Authors: Carnevali Luca , Statello Rosario , Sgoifo Andrea
Published: May 2019

The electrical stability of the myocardium is dependent on the dynamic balance between sympathetic and parasympathetic influences on the heart, which is reflected by heart rate variability (HRV). Reduced HRV is a proposed predictor of sudden death caused by ventricular tachyarrhythmias in cardiac patients. However, the link between individual differences in HRV and ventricular tachyarrhythmic risk in populations without known pre-existing cardiac conditions is less well explored. In this study we investigated the extent to which individual differences in resting state HRV predict susceptibility to spontaneous and pharmacologically-induced ventricular arrhythmias in healthy rats. Radiotelemetric transmitters were implanted in 42 adult male Wild-type Groningen rats. ECG signals were recorded during 24-h resting conditions and under β-adrenoceptor pharmacological stimulation with isoproterenol and analyzed by means of time- and frequency-domain indexes of HRV. No significant association was found between individual differences in resting measures of HRV and spontaneous incidence of ventricular arrhythmias. However, lower resting values of HRV predicted a higher number of ventricular ectopic beats following β-adrenergic pharmacological stimulation with isoproterenol (0.02 mg/kg). Moreover, after isoproterenol administration, one rat with low resting HRV developed sustained ventricular tachycardia that led to death. The present results might be indicative of the potential utility of HRV measures of resting cardiac autonomic function for the prediction of ventricular arrhythmias, particularly during conditions of strong sympathetic activation, in populations without known cardiac disease.
Potential Effects of CXCL9 and CCL20 on Cardiac Fibrosis in Patients with Myocardial Infarction and Isoproterenol-Treated Rats
Authors: Lin Chao-Feng , Su Chih-Jou , Liu Jia-Hong , Chen Shui-Tien , Huang Han-Li , Pan Shiow-Lin
Published: May 2019

The chemokines CXCL9 and CCL20 have been reported to be associated with ventricular dysfunction. This study was aimed to investigate the effects of CXCL9/CCL20 on cardiac fibrosis following myocardial infarction (MI). Blood samples of patients with MI were obtained to determine the serum CXCL9, CCL20, tumor necrosis factor-α (TNF-α), and transforming growth factor-β (TGF-β). The expression of CXCL9 and CCL20 in hypoxia-incubated H9c2 cells and TNF-α/TGF-β-activated peripheral blood mononuclear cells (PBMCs) were examined. The experimental MI of rats was produced by the intraperitoneal injection of isoproterenol (ISO) (85 mg/kg/day) for two consecutive days. The growth and migration of CXCL9/CCL20-incubated cardiac fibroblasts in vitro were evaluated. TNF-α/TGF-β-activated PBMCs showed an enhanced expression of CXCL9 and CCL20, while hypoxic H9c2 cells did not. Patients with MI had significantly enhanced levels of serum TGF-β and CXCL9 compared to healthy subjects. ISO-treated rats had increased serum CXCL9 levels and marked cardiac fibrosis compared to control rats. The trend of increased serum CCL20 in patients with MI and ISO-treated rats was not significant. CXCL9-incubated cardiac fibroblasts showed enhanced proliferation and migration. The findings of this study suggest that an enhanced expression of CXCL9 following MI might play a role in post-MI cardiac fibrosis by activating cardiac fibroblasts.
Maternal Age and Pregnancy, Childbirth and the Puerperium: Obstetric Results
Authors: Molina-García Leticia , Hidalgo-Ruiz Manuel , Arredondo-López Beatriz , Colomino-Ceprián Silvia , Delgado-Rodríguez Miguel , Martínez-Galiano Juan Miguel
Published: May 2019

Delaying maternity is becoming more common, resulting in questions regarding the influence age may have on obstetric results. Therefore, we proposed the objective to determine the association between maternal age and different health variables during pregnancy, childbirth and the puerperium. We conducted an observational study in Spain with primiparous women in which data was collected on sociodemographic, health and obstetric variables. Crude and adjusted mean differences were calculated with their corresponding 95% confidence intervals. The study included a final sample of 373 women. The mean age of women presenting with hypertension during pregnancy was 34.54 years (95% CI: 31.80–37.27) compared with a mean of 30.11 (95% CI: 29.56–30.66) in women that did not (p = 0.002). Women who had a eutocic delivery were also younger: 29.17 years (95% CI: 28.48–29.86) compared with 31.90 years (95% CI: 31.05–32.74; p < 0.001) for those that had a dystocic delivery. The duration of dilatation was longer in those ≥35 years (p = 0.001). In conclusion, an advanced maternal age is associated with a higher incidence of pathology during pregnancy and dystocic labor.
A tRNA- and Anticodon-Centric View of the Evolution of Aminoacyl-tRNA Synthetases, tRNAomes, and the Genetic Code
Authors: Kim Yunsoo , Opron Kristopher , Burton Zachary F.
Published: May 2019

Pathways of standard genetic code evolution remain conserved and apparent, particularly upon analysis of aminoacyl-tRNA synthetase (aaRS) lineages. Despite having incompatible active site folds, class I and class II aaRS are homologs by sequence. Specifically, structural class IA aaRS enzymes derive from class IIA aaRS enzymes by in-frame extension of the protein N-terminus and by an alternate fold nucleated by the N-terminal extension. The divergence of aaRS enzymes in the class I and class II clades was analyzed using the Phyre2 protein fold recognition server. The class I aaRS radiated from the class IA enzymes, and the class II aaRS radiated from the class IIA enzymes. The radiations of aaRS enzymes bolster the coevolution theory for evolution of the amino acids, tRNAomes, the genetic code, and aaRS enzymes and support a tRNA anticodon-centric perspective. We posit that second- and third-position tRNA anticodon sequence preference (C>(U~G)>A) powerfully selected the sectoring pathway for the code. GlyRS-IIA appears to have been the primordial aaRS from which all aaRS enzymes evolved, and glycine appears to have been the primordial amino acid around which the genetic code evolved.
Curdepsidones B–G, Six Depsidones with Anti-Inflammatory Activities from the Marine-Derived Fungus Curvularia sp. IFB-Z10
Authors: Ding Yi , An Faliang , Zhu Xiaojing , Yu Haiyuan , Hao Liling , Lu Yanhua
Published: May 2019

Six new depsidones, curdepsidones B–G (1–6), were obtained from the marine-derived fungus Curvularia sp. IFB-Z10. Their planar structures were determined by comprehensive analysis of HRESIMS and 1D/2D-NMR data. The absolute configuration of curdepsidones B–C (1–2) were established by synergistic use of DFT/NMR (density functional theory/nuclear magnetic resonance) and TDDFT/ECD (time-dependent density functional theory/electronic circular dichroism) calculations. Partial isolated compounds were tested for their anti-inflammatory activities in Propionibacterium acnes-induced THP-1 cells. Curdepsidone C (2) displayed significant anti-inflammatory properties with an IC50 value of 7.47 ± 0.35 μM, and reduced the P. acnes-induced phosphorylation levels of JNK and ERK in a dose-dependent mechanism. The possible anti-inflammatory mechanism of 2 was also investigated by molecular docking.
Marine Natural Products from Microalgae: An -Omics Overview
Authors: Lauritano Chiara , Ferrante Maria Immacolata , Rogato Alessandra
Published: May 2019

Over the last decade, genome sequences and other -omics datasets have been produced for a wide range of microalgae, and several others are on the way. Marine microalgae possess distinct and unique metabolic pathways, and can potentially produce specific secondary metabolites with biological activity (e.g., antipredator, allelopathic, antiproliferative, cytotoxic, anticancer, photoprotective, as well as anti-infective and antifouling activities). Because microalgae are very diverse, and adapted to a broad variety of environmental conditions, the chances to find novel and unexplored bioactive metabolites with properties of interest for biotechnological and biomedical applications are high. This review presents a comprehensive overview of the current efforts and of the available solutions to produce, explore and exploit -omics datasets, with the aim of identifying species and strains with the highest potential for the identification of novel marine natural products. In addition, funding efforts for the implementation of marine microalgal -omics resources and future perspectives are presented as well.
Widespread Existence of Quorum Sensing Inhibitors in Marine Bacteria: Potential Drugs to Combat Pathogens with Novel Strategies
Authors: Zhao Jing , Li Xinyun , Hou Xiyan , Quan Chunshan , Chen Ming
Published: May 2019

Quorum sensing (QS) is a phenomenon of intercellular communication discovered mainly in bacteria. A QS system consisting of QS signal molecules and regulatory protein components could control physiological behaviors and virulence gene expression of bacterial pathogens. Therefore, QS inhibition could be a novel strategy to combat pathogens and related diseases. QS inhibitors (QSIs), mainly categorized into small chemical molecules and quorum quenching enzymes, could be extracted from diverse sources in marine environment and terrestrial environment. With the focus on the exploitation of marine resources in recent years, more and more QSIs from the marine environment have been investigated. In this article, we present a comprehensive review of QSIs from marine bacteria. Firstly, screening work of marine bacteria with potential QSIs was concluded and these marine bacteria were classified. Afterwards, two categories of marine bacteria-derived QSIs were summarized from the aspects of sources, structures, QS inhibition mechanisms, environmental tolerance, effects/applications, etc. Next, structural modification of natural small molecule QSIs for future drug development was discussed. Finally, potential applications of QSIs from marine bacteria in human healthcare, aquaculture, crop cultivation, etc. were elucidated, indicating promising and extensive application perspectives of QS disruption as a novel antimicrobial strategy.
Edaravone-Loaded Alginate-Based Nanocomposite Hydrogel Accelerated Chronic Wound Healing in Diabetic Mice
Authors: Fan Ying , Wu Wen , Lei Yu , Gaucher Caroline , Pei Shuchen , Zhang Jinqiang , Xia Xuefeng
Published: May 2019

Refractory wound healing is one of the most common complications of diabetes. Excessive production of reactive oxygen species (ROS) can cause chronic inflammation and thus impair cutaneous wound healing. Scavenging these ROS in wound dressing may offer effective treatment for chronic wounds. Here, a nanocomposite hydrogel based on alginate and positively charged Eudragit nanoparticles containing edaravone, an efficient free radical scavenger, was developed for maximal ROS sequestration. Eudragit nanoparticles enhanced edaravone solubility and stability breaking the limitations in application. Furthermore, loading these Eudragit nanoparticles into an alginate hydrogel increased the protection and sustained the release of edaravone. The nanocomposite hydrogel is shown to promote wound healing in a dose-dependent way. A low dose of edaravone-loaded nanocomposite hydrogel accelerated wound healing in diabetic mice. On the contrary, a high dose of edaravone might hamper the healing. Those results indicated the dual role of ROS in chronic wounds. In addition, the discovery of this work pointed out that dose could be the key factor limiting the translational application of antioxidants in wound healing.
Experimental and FEM Analysis of Void Closure in the Hot Cogging Process of Tool Steel
Authors: Kukuryk Marcin
Published: May 2019

In the present study, a new complex methodology for the analysis the closure of voids and a new forging system were developed and tested. The efficiency of the forging parameters and the effective geometric shapes of anvils to improve void closure were determined. A new cogging process provided a complete closure of an ingot’s axial defects, as confirmed by experimental tests. The evolution behavior of these defects with different sizes was investigated during the hot cogging process by means of the professional plastic forming software Deform-3D. A comprehensive procedure was developed using the finite-element method (FEM) for the three-dimensional cogging process and laboratory experimentation to predict the degree of void closure. The hot multi-pass cogging process was used to eliminate void defects in the forgings so as to obtain sound products. In the compression process, the effects of the reduction ratio and forging ratio, the void size, and the types of anvil were discussed to obtain the effective elimination of a void. For the purpose of the assessment of the effectiveness of the void closure process, the following indices were introduced: the relative void volume evolution ratio, the relative void diameter ratio, and the internal void closure evaluation index. Moreover, the void closure process was assessed on the basis of stress triaxiality, hydrostatic stress, forging ratio, value of local effective strain around the void, and critical reduction ratio. The results of this research were complemented by experiments predicting the formation of fractures in the regions near the void and in the volume of the forging in the course of the cogging process. The comparison between the predicted and the experimental results showed a good agreement.
Induction Skull Melting of Ti-6Al-4V: Process Control and Efficiency Optimization
Authors: Chamorro Xabier , Herrero-Dorca Nuria , Bernal Daniel , Hurtado Iñaki
Published: May 2019

Titanium investment casting is one of the leading and most efficient near-net-shape manufacturing processes, since complex shape components are possible to obtain with a very low amount of material waste. But melting these reactive alloys implies the usage of specific melting technologies such as the Induction Skull Melting (ISM) method. In this work the ISM was extensively studied with the aim of deepening the characteristics of this specific melting method and improving the too low energy efficiency and overall process performance. A 16 segment copper crucible and 3 turns coil was employed for the melting of 1 kg of Ti-6Al-4V alloy. Through the calorimetric balance, real-time evolution of the process parameters and power losses arising from the crucible and coil sub-assemblies was displayed. Results revealed the impact of coil working conditions in the overall ISM thermal efficiency and titanium melt properties, revealing the use of these conditions as an effective optimization strategy. This unstudied melting control method allowed more heat into charge and 13% efficiency enhancement; leading to a shorter melting process, less energy consumption and increased melt superheat, which reached 49 °C. The experimental data published in this paper represent a valuable empiric reference for the development and validation of current and future induction heating models.
Cyclic Indentation of Iron: A Comparison of Experimental and Atomistic Simulations
Authors: Deldar Shayan , Alabd Alhafez Iyad , Smaga Marek , Beck Tilmann , Urbassek Herbert M.
Published: May 2019

Cyclic indentation is a technique used to characterize materials by indenting repeatedly on the same location. This technique allows information to be obtained on how the plastic material response changes under repeated loading. We explore the processes underlying this technique using a combined experimental and simulative approach. We focus on the loading–unloading hysteresis and the dependence of the hysteresis width ha,p on the cycle number. In both approaches, we obtain a power-law demonstrating ha,p with respect to the hardening exponent e. A detailed analysis of the atomistic simulation results shows that changes in the dislocation network under repeated indentation are responsible for this behavior.
On the Free-Surface Roughness in Incremental Forming of a Sheet Metal: A Study from the Perspective of ISF Strain, Surface Morphology, Post-Forming Properties, and Process Conditions
Authors: Al-Ghamdi Khalid A , Hussain G.
Published: May 2019

Due to absence of any supporting die, the free surfaces in incremental sheet forming (ISF) experience uneven deformation. This results in rough surfaces, possibly leading to the reduced service life of components. Therefore, it is necessary to analyze and quantify the effects of the application of strain on the free-surface roughness. Moreover, in order to control roughness, both on the free surface and the opposite contact surface, the nature of correlation between the two types of roughnesses needs to be identified by classifying the significance of different process conditions. The present work is a fundamental study to address these points. A series of specimens are produced by subjecting a metallic sheet to a range of ISF strains (13% to 98%). These specimens are then subjected to a number of characterization tests, namely roughness, uniaxial tension, and residual stress tests. The results reveal that the mean free-surface roughness increases non-linearly as the normal strain (stretching + bending) on the free surface increases (where strain state on the surface is as follows: ɛ1 = 0, ɛ2 > 0, γmax = ɛ2 and 1 and 2 are principal directions). The roughness also increases, although linearly, with the post-forming sheet strength, residual stress, and forming force, thereby showing that strain hardening has a direct influence on the roughness in a way that sheet strengthening is achieved at the cost of surface quality. The surface morphology reveals that the free surfaces contained orange peel, slip lines, and micro-voids, with density increasing with strain application, thus indicating the possible influence of tensile stresses on free surface deformation and roughening at an increasing degree with strain. Further analysis of roughness results discloses that the free-surface roughness and the contact-surface roughness are inversely related, because the responses of the two to ISF processing were mutually exclusive. Based on the obtained results, future research directions are also discussed.
Effect of Differentiated Injection Ratio, Gas Flow Rate, and Slag Thickness on Mixing Time and Open Eye Area in Gas-Stirred Ladle Assisted by Physical Modeling
Authors: Jardón-Pérez Luis E. , González-Morales Daniel R. , Trápaga Gerardo , González-Rivera Carlos , Ramírez-Argáez Marco A.
Published: May 2019

In this work, the effects of equal (50%/50%) or differentiated (75%/25%) gas flow ratio, gas flow rate, and slag thickness on mixing time and open eye area were studied in a physical model of a gas stirred ladle with dual plugs separated by an angle of 180°. The effect of the variables under study was determined using a two-level factorial design. Particle image velocimetry (PIV) was used to establish, through the analysis of the flow patterns and turbulence kinetic energy contours, the effect of the studied variables on the hydrodynamics of the system. Results revealed that differentiated injection ratio significantly changes the flow structure and greatly influences the behavior of the system regarding mixing time and open eye area. The Pareto front of the optimized results on both mixing time and open eye area was obtained through a multi-objective optimization using a genetic algorithm (NSGA-II). The results are conclusive in that the ladle must be operated using differentiated flow ratio for optimal performance.
NMR-Based Tissular and Developmental Metabolomics of Tomato Fruit
Authors: Lemaire-Chamley Martine , Mounet Fabien , Deborde Catherine , Maucourt Mickaël , Jacob Daniel , Moing Annick
Published: May 2019

Fruit is a complex organ containing seeds and several interconnected tissues with dedicated roles. However, most biochemical or molecular studies about fleshy fruit development concern the entire fruit, the fruit without seeds, or pericarp only. We studied tomato (Solanum lycopersicum) fruit at four stages of development (12, 20, 35, and 45 days post-anthesis). We separated the seeds and the other tissues, exocarp, mesocarp, columella with placenta and locular tissue, and analyzed them individually using proton NMR metabolomic profiling for the quantification of major polar metabolites, enzymatic analysis of starch, and LC-DAD analysis of isoprenoids. Pericarp tissue represented about half of the entire fruit mass only. The composition of each fruit tissue changed during fruit development. An ANOVA-PCA highlighted common, and specific metabolite trends between tissues e.g., higher contents of chlorogenate in locular tissue and of starch in columella. Euclidian distances based on compositional data showed proximities within and between tissues. Several metabolic regulations differed between tissues as revealed by the comparison of metabolite networks based on correlations between compounds. This work stressed the role of specific tissues less studied than pericarp but that impact fruit organoleptic quality including its shape and taste, and fruit processing quality.
Thermal Alteration of Organic Matter in the Contact of a Rift-Related Basaltic Dyke: An Example from the Black Limestone, Wadi Matulla, West Central Sinai, Egypt
Authors: Abu Sharib Ahmed S. A. A. , Selim Ali Q. , Abdel Fattah Mohamed M. , Hassan Safiya M. , Sanislav Ioan V.
Published: May 2019

In the Wadi Matulla area, central Sinai, Egypt, an asymmetric baked zone having an average width of 103 m was formed on both sides of a sub-aerial rift-related Oligocene basaltic dyke cross-cutting organic matter-bearing chalky limestone of the Upper Cretaceous Sudr Formation. Advection was the significant heat transfer mechanism. Very narrow metamorphic and metasomatic zones are developed in the country rock at the immediate contact with the dyke. The change in the thermal maturation of organic matter is reflected in the differences in values of the total organic carbon (TOC) within the baked zone. Such differences account for the color variation of the snow-white limestone from shades of brown, in the mature to barren samples, to black, in the totally carbonized overmature metamorphic ones. This study presents for the first time the thermal effect of mafic dykes on some exposed organic matter-bearing rocks in the Gulf of Suez (GOS) region, and turns attention to the local maturation of source rocks in contact with rift-related intrusives at a relatively greater burial depth in the rift basin.