bluuebunny/crossref_metadata_2025 huggingface dataset:
How farmers think about insects: perceptions of biodiversity, biodiversity loss and attitudes towards insect-friendly farming practices
Authors: Busse Maria , Zoll Felix , Siebert Rosemarie , Bartels Annette , Bokelmann Anke , Scharschmidt Phillipp
Published: July 2021

An alarming decrease of insects in number and variety calls for measures of protection and promotion, since insects are crucial for the functioning of ecosystems and provide multiple ecosystem services. Agricultural landscapes can provide vast insect habitats if they are managed accordingly. However, little is known about farmers’ problem awareness and attitudes toward insect biodiversity loss, related farming practises, or alternative acceptable insect-friendly solutions. To fill these research gaps, this paper aimed to reveal farmers’ perceptions and attitudes regarding these aspects in two German case studies. We conducted 23 semi-structured interviews with farmers in 2019 and qualitatively analysed them using semantic web analysis. Farmers mostly reported awareness of insects’ ecosystem services and disservices related to agricultural production rather than mentioning the holistic ecological importance of insects. About half of the farmers confirmed insect loss based on their own observations, whereas a similar number doubted there had been a decrease of insects. Most farmers are open-minded towards insect-friendly measures if financially compensated. The farmers also mentioned a joint societal responsibility for insects, economic pressure on farmers to use pesticides due to global market prices, and unbalanced agricultural policies. This study revealed in-depth insights into farmers’ thinking about insects and how farmers contextualise arguments. Our results identified overlaps in farmers’ mental models, which paves the way for co-designing insect-friendly farming practices in landscape labs. Local transformation efforts can also demonstrate new pathways for a shift on the higher levels.
Ontological Pluralism and Multi-Quantificational Ontology
Authors: Król Zbigniew , Lubacz Józef
Published: July 2021

This paper explores some variants and aspects of multi-quantificational criteria of existence, examining these in the context of the debate between monism and pluralism in analytical philosophy. Assuming familiarity with the findings to date (summarized in broad terms at the outset), we seek to apply to these the newly introduced concepts of “substitution” and “substitutional model”. Possible applications of formal theories involving multiple types of existential quantifier are highlighted, together with their methods of construction. These considerations then lead to a thesis asserting the irrelevance of both multi-quantificational criteria and assumptions involving quantificational ontology to the debate between monism and pluralism in ontology. Many quantifiers cannot properly distinguish different modes of existence–as we aim to show by furnishing a general method for constructing counter-examples to any theory that assumes that different types of existential quantifier correspond to different modes of existence.
Lobbying for size and slice of the quota
Authors: Lappi Pauli
Published: July 2021

The formation and allocation of an emission quota are analyzed in a common agency framework with two stages. First, the principals lobby for the size of the aggregate quota. Second, the principals lobby for the individual slices of the quota. It is shown that the slices are allocated such that the marginal profits of the principals are equalized and that the size of the aggregate quota is either set at the efficient level characterized by the Samuelson’s rule for public goods or distorted from that level. When the quota is distorted from the efficient level it is set such that the aggregate marginal profit is less than the marginal damage, resulting in an overallocation of individual and aggregate quotas. However, efficient level of the quota is obtained in a reasonable special case in which countries take the role of the principals. The results are extended to cover tradable emission permits.
Pathways to Mental Well-Being in Young Carers: The Role of Benefit Finding, Coping, Helplessness, and Caring Tasks
Authors: Wepf Hannah , Joseph Stephen , Leu Agnes
Published: July 2021

Although prior research has shown that young carers may perceive benefits from their challenging situation, it is unclear how and when benefit finding leads to better mental health. This study examines pathways through which benefit finding may influence mental well-being. Self-reported data were obtained from 601 adolescents aged 15–21 (Mage = 17.87, 71.9% female) who provided care for a close person with physical or mental health problems. Benefit finding was associated with better mental well-being directly as well as indirectly via better coping and lower helplessness. These findings were similar across young carers with different caring task profiles, except for a few differences regarding social/emotional and instrumental care. The study suggests that benefit finding could promote coping skills and mental well-being in adolescent young carers with implications for the design of future research on interventions with young carers.
Vertical Mergers and Input Foreclosure Lessons from the AT&T/Time Warner Case
Authors: Shapiro Carl
Published: July 2021

This article offers a practical guide to analyzing vertical mergers using the general approach to input foreclosure and raising rivals’ costs that is described in the 2020 Vertical Merger Guidelines that were issued by the U.S. Department of Justice and the Federal Trade Commission. The step-by-step analysis described here draws lessons from how that theory of harm played out in the lone vertical merger case that has been litigated by the antitrust agencies in recent decades: the 2018 challenge by the Department of Justice to the merger between AT&T and Time Warner. I testified in court as the DOJ’s economic expert in that case. I explain here how to quantify the increase in rivals’ costs and the elimination of double marginalization that are caused by a vertical merger and how to evaluate their net effect on downstream customers. I also explain how this economic analysis fits into the three-step burden-shifting approach that the courts apply to mergers under Section 7 of the Clayton Act. Based on my experience in the AT&T/Time Warner case, I identify a number of shortcomings of the 2020 Vertical Merger Guidelines.
Perceptual justification in the Bayesian brain: a foundherentist account
Authors: Gładziejewski Paweł
Published: July 2021

In this paper, I use the predictive processing (PP) theory of perception to tackle the question of how perceptual states can be rationally involved in cognition by justifying other mental states. I put forward two claims regarding the epistemological implications of PP. First, perceptual states can confer justification on other mental states because the perceptual states are themselves rationally acquired. Second, despite being inferentially justified rather than epistemically basic, perceptual states can still be epistemically responsive to the mind-independent world. My main goal is to elucidate the epistemology of perception already implicit in PP. But I also hope to show how it is possible to peacefully combine central tenets of foundationalist and coherentist accounts of the rational powers of perception while avoiding the well-recognized pitfalls of either.
Religious Conversion and Loss of Faith: Cases of Personal Paradigm Shift?
Authors: Le Poidevin Robin
Published: July 2021

Is Thomas Kuhn’s model of scientific revolutions in terms of paradigm shifts appropriately applied to cases of radical changes in religious outlook, and in particular conversion to faith, or loss of faith? Since this question cannot be addressed in purely a priori terms, three case studies of philosophers who have described significant changes in their own perspectives are examined. Part of the justification for such an approach is to see how changes in view seem from the first-person perspective. Although what is offered here is a very limited group of studies, each of them perforce brief, a key theme emerges: that the incommensurability between paradigms which is part of the Kuhnian model is mirrored by the shift away from atheist or humanistic perspectives, though not necessarily by loss of faith.
Novel agents in a young patient with osteosarcoma: A short review
Authors: Steinbrecher Oskar , Brodowicz Thomas , Lamm Wolfgang
Published: July 2021

Osteosarcomas are rare malignant bone tumors, most frequently occurring in children as well as adolescents and young adults. Therapy of initially localized disease consists of neoadjuvant chemotherapy followed by surgical resection and adjuvant chemotherapy. Osteosarcomas often present relapses, most commonly lung metastases. Treatment of isolated lung metastasis most commonly includes surgical resection. The correct adjuvant treatment option is still under investigation. In this manuscript we describe the clinical course of an osteosarcoma patient and give a review of the literature regarding current standard treatment for localized as well as pulmonary metastatic disease.
Sustainable manufacturing of ultra-fine aluminium alloy 6101 wires using controlled high levels of mechanical strain and finite element modeling
Authors: Caruso Serafino , Filice Luigino
Published: July 2021

The evolution of grain size and component mechanical behaviour are fundamental aspects to analyse and control when manufacturing processes are considered. In this context, severe plastic deformation (SPD) processes, in which a high shear strain is imposed on the material, are recognized as the main techniques to achieve microstructural changes and material strengthening by the recrystallization, attracting both academic and industrial investigation activities. At the same time, nowadays, sustainable manufacturing design is one of the main responsibilities of the researchers looking at UN2030 agenda and the modern industrial paradigms. In this paper a new severe SPD process is proposed with the aim to steer manufacturing to fourth industrial revolution using some of Industry 4.0 pillars. In particular, additive manufacturing (AM) and numerical simulations were setup as controlling and monitoring techniques in manufacturing process of wires. Strengthening effect (yield and ultimate tensile strength, plasticity and hardness) and microstructural evolution (continuous dynamic recrystallization -CDRX-) due to severe plastic deformation were experimentally analysed and numerically investigated by an innovative finite element (FE) model able to validate the effectiveness of a properly modified process for ultra-fine aluminium alloy AA6101 wires production designed with the aim to avoid any post manufacturing costly thermal treatment. The study provides an accurate experimental study and numerical prediction of the thermo-mechanical and microstructural phenomena that occur during an advanced large plastic deformation process; it shows how the combination of smart manufacturing and simulations control represents the key to renew the traditional manufacturing methods in the perspective of the Industry 4.0, connecting and integrating the manufacturing process for the industrial evolution in production.
The prognostic significance of primary tumor size in squamous cell carcinoma of the penis
Authors: Li Kai , Wu Guang , Fan Caibin , Yuan Hexing
Published: July 2021

Background

To evaluate the association of primary tumor size with clinicopathologic characteristics and survival of patients with squamous cell carcinoma of the penis (SCCP).

Methods

This study analyzed the data of 1001 patients with SCCP, obtained from the National Cancer Institute Surveillance, Epidemiology, and End Results (SEER) database between 2010 and 2014. The Kaplan–Meier method and the Cox proportional hazards regression model were used to analyze the effects of primary tumor size on overall survival (OS) and penile carcinoma-specific survival (PCSS).

Results

Advanced T stage (P < 0.001), lymph node metastasis (P < 0.001) and distant metastasis (P = 0.001) were more frequently associated with SCCP patients with tumor size ≥ 3 cm than those with tumor size  < 3 cm. In Kaplan–Meier analyses, the patients with large tumors (≥ 3 cm) exhibited an inferior OS and PCSS than those with small tumors (< 3 cm). Moreover, tumor size was identified to be an independent prognostic factor for OS [hazard ratio (HR) 1.665, P < 0.001] and PCSS (HR 2.076, P = 0.003) of patients with SCCP in multivariate analyses.

Conclusions

Large tumor size is associated with adverse clinicopathological characteristics of patients with SCCP. Besides, tumor size represents an independent prognostic factor for OS and PCSS. Therefore, clinical assessment of tumor size as a crucial prognostic factor might be highly beneficial for early intervention in patients with SCCP.
Discovering Knowledge and Cognitive Based Drivers for SMEs Internationalization
Authors: Alinasab Jamshid , Mirahmadi Seid Mohammad Reza , Ghorbani Hassan , Caputo Francesco
Published: July 2021

Internationalisation begins with companies’ decision to enter global markets to develop knowledge and experience as key competitive factors in the global economy, which has been the subject of much empirical research. Decision-making, knowledge management, and effective internationalisation have become key strategic tools for all companies, especially for small- and medium-sized enterprises (SMEs). This study wants to provide a framework for SMEs internationalisation based on the real options theory, (ROT) as a knowledge driver method. For this purpose, the effective factors for internationalisation were identified by reviewing the literature on the subject and the internal and external backgrounds of the subject. Then, main and sub-factors were prioritised by the fuzzy analytic hierarchy process (AHP) method. The statistical population consisted of senior managers, business managers of SMEs in Isfahan, Iran, who were eligible to enter this study. Twenty-six experts participated in this study by judgmental non-random sampling method. A fuzzy AHP questionnaire was prepared in the form of 19 sub-factors and 7 main factors. The components of each factor in each group were also ranked by experts, and their weights were obtained. Next, according to the ROT strategies which have 5 options, an alignment matrix was used to align the factors affecting the decision with the strategies. After answering the research question, the option that had a higher mode was consideredAS; then, this score was multiplied by the weight obtained in the previous step and theTASwas obtained. Finally, strategies were classified as appropriate, need further investigation, and inappropriate.
The Sibling Distribution for Multivariate Life Time Data
Authors: Helgøy Ingvild M. , Skaug Hans J.
Published: July 2021

A flexible class of multivariate distributions for continuous lifetimes is proposed. The distribution is defined in terms of the age-at-death of m siblings. The expression for the joint density is derived using classical results from mathematical demography. The parameters of the distribution are the age-specific birth and death rates, in addition to a vector of relative death times for the m siblings. For the case of constant birth and death rates we are able to derive an explicit expression for the bivariate sibling density, which is proven to be MTP2, and hence has positive dependence. Further, we show that a special case of the sibling distribution belongs to the Block-Basu class of multivariate distribution. In the general case, with age-dependent birth and death rates, evaluation of the density involves numerical integration, but is still feasible.
Lifetime predictions of non-ionic and ionic biopolymers: kinetic studies by non-isothermal thermogravimetric analysis
Authors: Calvino Martina Maria , Lisuzzo Lorenzo , Cavallaro Giuseppe , Lazzara Giuseppe , Milioto Stefana
Published: July 2021

In this paper, films based on sustainable polymers with variable charge have been investigated by non-isothermal thermogravimetry in order to predict their lifetime, which is a key parameter for their potential use in numerous technological and biomedical applications. Specifically, chitosan has been selected as positively charged biopolymer, while alginate has been chosen as negatively charged biopolymer. Among non-ionic polymers, methylcellulose has been investigated. Thermogravimetric measurements at variable heating rates (5, 10, 15 and 20 °C min−1) have been performed for all the polymers to study their degradation kinetics by using isoconversional procedures combined with ‘Master plot’ analyses. Both integral (KAS and Starink methods) and differential (Friedman method) isoconversional procedures have shown that chitosan possesses the highest energetic barrier to decomposition. Based on the Master plot analysis, the decomposition of ionic polymers can be described by the R2 kinetic model (contracted cylindrical geometry), while the degradation of methylcellulose reflects the D2 mechanism (two-dimensional diffusion). The determination of both the decomposition mechanism and the kinetic parameters (activation energy and pre-exponential factor) has been used to determine the decay time functions of the several biopolymers. The obtained insights can be helpful for the development of durable films based on sustainable polymers with variable electrostatic characteristics.

Graphical abstract
In Fortune's Theater
Authors: Baker Nicholas Scott
Published: July 2021

This innovative cultural history of financial risk-taking in Renaissance Italy argues that a new concept of the future as unknown and unknowable emerged in Italian society between the mid-fifteenth and mid-sixteenth centuries. Exploring the rich interchanges between mercantile and intellectual cultures underpinning this development in four major cities - Florence, Genoa, Venice, and Milan - Nicholas Scott Baker examines how merchants and gamblers, the futurologists of the pre-modern world, understood and experienced their own risk taking and that of others. Drawing on extensive archival research, this study demonstrates that while the Renaissance did not create the modern sense of time, it constructed the foundations on which it could develop. The new conceptions of the past and the future that developed in the Renaissance provided the pattern for the later construction a single narrative beginning in classical antiquity stretching to the now. This book thus makes an important contribution toward laying bare the historical contingency of a sense of time that continues to structure our world in profound ways.
Evolution of forced magnetohydrodynamic waves in a stratified fluid
Authors: Sreenivasan Binod , Maurya Gaurav
Published: July 2021

The evolution of a buoyancy disturbance in a stratified incompressible fluid permeated by a uniform vertical magnetic field is investigated. Two regimes are considered in the absence of background rotation – that of strong stratification, where the internal gravity wave frequency

$\omega _A$

is much higher in magnitude than the magnetic (Alfvén) wave frequency

$\omega _M$

, and that of strong magnetic field, where

$\omega _M$

is dominant. For small but finite magnetic diffusion, perturbations that initially lie in the strong-field regime are shown to cross over to the regime of strong stratification, so that small-scale motions may exist as damped internal gravity waves at large times. The induced magnetic field propagates as damped Alfvén waves for a much longer time than the velocity before undergoing the above transition. With strong rotation, the unstably stratified system that satisfies the inequality

$|\omega _C| > |\omega _M| \gg |\omega _A| \gg |\omega _\eta |$

, where

$\omega _C$

is the inertial wave frequency and

$\omega _\eta$

is the diffusion frequency, is of relevance to convection-driven dynamos. Here, a parameter space with

$|\omega _M/\omega _C| \sim 0.1$

is found wherein the flow intensity of the slow magnetic-Archimedean-Coriolis (MAC) waves is of the same order of magnitude as that of the fast MAC waves. Slow wave motions at horizontal length scales much smaller than the width of the fluid layer can therefore generate substantial helicity in rapidly rotating dynamos. The excitation of slow MAC waves at scales of

$\sim$

10 km in the Earth's core may play a crucial role in the generation of the axial dipole field.
Gender, Candidate Emotional Expression, and Voter Reactions During Televised Debates
Authors: BOUSSALIS CONSTANTINE , COAN TRAVIS G. , HOLMAN MIRYA R. , MÜLLER STEFAN
Published: July 2021

Voters evaluate politicians not just by what they say, but also how they say it, via facial displays of emotions and vocal pitch. Candidate characteristics can shape how leaders use—and how voters react to—nonverbal cues. Drawing on role congruity expectations, we study how the use of and reactions to facial, vocal, and textual communication in political debates varies by candidate gender. Relying on full-length videos of four German federal election debates (2005–2017) and a minor party debate, we use video, audio, and text data to measure candidate facial displays of emotion, vocal pitch, and speech sentiment. Consistent with our expectations, Angela Merkel expresses less anger than her male opponents, but she is just as emotive in other respects. Combining these measures of emotional expression with continuous responses recorded by live audiences, we find that voters punish Merkel for anger displays and reward her happiness and general emotional displays.
Carbon isotope chemostratigraphy and sea-level history of the Hirnantian Stage (uppermost Ordovician) in the Oslo–Asker district, Norway
Authors: Calner Mikael , Bockelie Johan Fredrik , Rasmussen Christian M.Ø. , Calner Hanna , Lehnert Oliver , Joachimski Michael M.
Published: July 2021

We present a δ13Ccarb chemostratigraphy for the Late Ordovician Hirnantian Stage based on 208 whole-rock samples from six outcrops in the Oslo–Asker district, southern Norway. Our data include the Norwegian type section for the Hirnantian Stage and Ordovician–Silurian boundary at Hovedøya Island. The most complete record of the Hirnantian Isotope Carbon Excursion (HICE) is identified in a coastal exposure at Konglungø locality where the preserved part of the anomaly spans a c. 24 m thick, mixed carbonate–siliciclastic succession belonging to the upper Husbergøya, Langåra and Langøyene formations and where δ13Ccarb peak values reach c. +6 ‰. Almost the entire HICE occurs above beds containing the Hirnantia Fauna, suggesting a latest Hirnantian age for the peak of the excursion. The temporal development of the HICE in southern Norway is associated with substantial shallowing of depositional environments. Sedimentary facies and erosional unconformities suggest four inferably fourth-order glacio-eustatically controlled sea-level lowstands with successively increased exposure and erosion to the succession. The youngest erosional unconformity is related to the development of incised valleys and resulted in cut-out of at least the falling limb of the HICE throughout most of the Oslo–Asker district. The fill of the valleys contains the falling limb of the HICE, and the postglacial transgression therefore can be assigned to the latest part of the Hirnantian Age. We address the recent findings of the chitinozoan Belonechitina gamachiana in the study area and its relationship to the first occurrence of Hirnantia Fauna in the studied sections, challenging identification of the base of the Hirnantian Stage.
Contextual reduction of word-final /l/ in Spanish: An EPG study
Authors: Ramsammy Michael
Published: July 2021

This article presents data on the contextual reduction of /l/ in Spanish. Electropalatography (EPG) was used to examine realisations of word-final /l/ in prevocalic and preconsonantal environments in order to determine to what extent articulatory reduction of the /l/ is attributable to coarticulation with following segments. Previous studies using static palatography (Josselyn 1907) describe continuous relaxation of the articulatory stricture associated with /l/ in different phonological environments. These descriptions, in turn, have informed standard reference works on Spanish phonetics (e.g. Navarro Tomás 1957, Gili Gaya 1966). Additionally, theoretical work on other languages has argued in favour of syllable-based accounts of /l/-allophony and reduction patterns (e.g. English /l/-darkening and vocalisation), whereas instrumental studies have revealed complexities to these patterns that challenge syllable-based analyses. The findings of the EPG study reported on here confirm that /l/-reduction in Spanish is a gradient phenomenon that arises due to antagonistic coarticulatory forces. Thus, the reduction patterns that emerge in the data cannot be predicted on the basis of syllabification algorithms alone.
Making the Representation Real: The Actor and the Spectator in Milo Rau’s ‘Theatrical Essays’ Mitleid and La Reprise
Authors: Young Stuart
Published: July 2021

Exceptional in demonstrating the political engagement emerging in twenty-first-century performance is the corpus of the writer and director Milo Rau, whose practice is distinguished by its (re)meditation of the real. With detailed reference to Mitleid (2016) and La Reprise (2018), this article examines Rau’s self-reflexive strategies in (re)presenting testimony or an event as a means not of depicting the real, but of making the theatrical representation itself real in order to change the world rather than merely to portray it. The article focuses in particular on strategies relating to the actor-character and spectatorship. Rau’s interest in the positions of the actor and spectator illuminates issues that have arisen in the discourse of theatre witnessing and in recent scholarship on dramaturgical approaches and spectatorship in contemporary political performance. Essentially, Rau makes the performer’s habitus transparent, and challenges the spectator’s reflexivity, effectively rebutting the largely unchallenged assumption that characters who perform witnesses necessarily leave little room for the spectator to be a performing witness. Stuart Young is Professor of Theatre Studies at the University of Otago. His recent publications include the co-edited Ethical Exchanges: Translation, Adaptation, Dramaturgy (Brill Rodopi, 2017), while his practice-led research into Theatre of the Real includes The Keys are in the Margarine: A Verbatim Play about Dementia (2014).
A Conversation on Directing Opera
Authors: Mitchell Katie , Frendo Mario
Published: July 2021

Katie Mitchell has been directing opera since 1996, when she debuted on the operatic stage with Mozart and Da Ponte’s Don Giovanni at the Welsh National Opera. Since then, she has directed more than twenty-nine operas in major opera houses around the world. Mitchell here speaks of her directorial approach when working with the genre, addressing various aspects of interest for those who want a better grasp of the dynamics of opera-making in the twenty-first century. Ranging from the director’s imprint, or signature on the work they put on the stage, to the relationships forged with people running opera institutions, Mitchell reflects on her experiences when staging opera productions. She sheds light on some fundamental differences between theatre-making and opera production, including the issue of text – the libretto, the dramatic text, and the musical score – and the very basic fact that in opera a director is working with singers, that is, with musicians whose attitude and behaviour on stage is necessarily different from that of actors in the theatre. Running throughout the conversation is Mitchell’s commitment to ensure that young and contemporary audiences do not see opera as a museum artefact but as a living performative experience that resonates with the aesthetics and political imperatives of our contemporary world. She speaks of the uncompromising political imperatives that remain central to her work ethic, even if this means deserting a project before it starts, and reflects on her long-term working relations with opera institutions that are open to new and alternative approaches to opera-making strategies. Mitchell underlines her respect for the specific rules of an art form that, because of its collaborative nature, must allow more space for theatre-makers to venture within its complex performative paths if it wants to secure a place in the future. Mario Frendo is Senior Lecturer of Theatre and Performance and Head of the Department of Theatre Studies at the School of Performing Arts, University of Malta, where he is the director of CaP, a research group focusing on the links between culture and performance.
Trying Again, Failing Again: Samuel Beckett and the Sequel Play
Authors: Simpson Hannah
Published: July 2021

Samuel Beckett’s Waiting for Godot has spawned several unauthorized sequel plays, which see Godot arrive on stage in 1960s Yugoslavia, 1980s Ireland, 1990s North America, and early 2000s Japan. The sequel play is a largely ignored phenomenon in literary scholarship, with the sequel form itself routinely dismissed as a derivative and inevitably disappointing text. Yet the sequel also re-situates and re-evaluates the original text, and its reiterative nature aptly parallels the paradox of non-ending in Beckett’s original Waiting for Godot. Focusing on four unauthorized stage sequels to Beckett’s play – Miodrag Bulatović’s Godo je došao (Godot Has Arrived, 1966), Alan Titley’s Tagann Godot (Godot Arrives, 1987), Daniel Curzon’s Godot Arrives (1999), and Minoru Betsuyaku’s Yattekita Godot (Godot Has Come, 2007) – this article examines how these sequels rework the cultural logic of Godot’s arrival to their own critical and political ends. These playwrights draw on the very recursive, even frustrating, nature of the sequel form itself as an exegetic framework, reproducing the trope of non-ending that characterizes Beckett’s own work. Hannah Simpson is the Rosemary Pountney Junior Research Fellow in British and European Drama at St Anne’s College, University of Oxford. She is currently working on two forthcoming Beckett-related monographs: Witnessing Pain: Samuel Beckett and Post-War Francophone Theatre and Samuel Beckett and Disability Performance.
BITEF Before and After 1989: Representation to Deconstruction of Social and Cultural Paradigms
Authors: Medenica Ivan
Published: July 2021

Ivan Medenica here analyzes the cultural shift that the Belgrade International Theatre Festival (BITEF) experienced after 1989. From its beginnings in the late 1960s until the end of the1980s, BITEF was a representation of the dominant multicultural, modernist, and progressive paradigm of Yugoslavia’s cultural policy. This was not an unambiguous position. On the one hand, modernist values were imposed by Tito’s authoritarian regime and, on the other, they were confronted with the conservative tendencies both in politics and the arts. As a multicultural and progressive platform, BITEF was one of the biggest victims in the field of the arts of Slobodan Milošević’s nationalist regime in the 1990s and the wars in former Yugoslavia. After the fall of Milošević in 2000, a complex period of tension started between the ‘reborn’ urge for democratization and internationalization, on the one hand, and persistent nationalism and conservatism, on the other. Due to its tradition, reinforced artistic ambitions, and international reputation, BITEF regained its fame. Its position today, however, is quite paradoxical. It is an anti-traditionalist and multicultural festival – within a culture and society that are becoming traditional and rather claustrophobic. Ivan Medenica is a Professor of Theatre at the University of the Arts in Belgrade in Serbia and has received the national award for theatre criticism six times. His publications include The Tragedy of Initiation, or the Inconstant Prince: The Classics and Their Masks. Medenica is also the artistic director of BITEF.
REGULATORY FIT EFFECTS ON THE ACQUISITION OF LEXICAL STRESS
Authors: Cho Minyoung
Published: July 2021

Higgins’s (2000) regulatory fit theory proposes that a fit between one’s regulatory state and strategic means for reaching a goal increases motivational strength and engagement. This study investigates how regulatory fit affects the L2 acquisition of lexical stress in an authentic learning context. Ninety EFL students were assigned to either gain frame or loss frame conditions. They engaged in speech practice in which they mimicked a model speech in English to win a chance to enter a prize raffle. The reward system was framed differently in the two framing conditions, with the intention of eliciting the participants’ use of eager or vigilant strategies, thus creating fit and nonfit conditions. Acquisition of lexical stress was assessed using pre- and posttest scores. Multiple regression analysis showed no regulatory fit effects and no loss frame effects but did show a significant beneficial effect of the gain frame on the acquisition of lexical stress.
A parametric approach to the acquisition of syntax
Authors: SNYDER William
Published: July 2021

Three case-studies, using longitudinal records of children's spontaneous speech, illustrate what happens when a child's syntax changes. The first, examining acquisition of English verb-particle constructions, shows a near-total absence of commission errors. The second, examining acquisition of prepositional questions in English or Spanish, shows that children (i) may go as long as 9 months producing both direct-object questions and declaratives with prepositional phrases, before first attempting a prepositional question; and (ii) at some point, abrubtly begin producing prepositional questions that are correctly formed for the target language. The third case study shows that in children acquiring English, the onset of verb-particle constructions occurs almost exactly when that child begins using novel noun-noun compounds. After a discussion of the implications for the nature of syntactic knowledge, and for the mechanisms by which it is acquired, two examples are presented of as-yet untested acquisitional predictions of parametric proposals in the syntax literature.
Mapping the Margins: National Borders, Transnational Networks and Images of Coherence in Interwar Italy and Yugoslavia
Authors: Worsnick Matthew
Published: July 2021

This article analyses the relationship between charted (drawn) maps and mental maps of the contested border-region between Italy and Yugoslavia following the First World War. Drawing upon graphic and verbal depictions of tourist routes, railways, supply chains, demographics, topographic features and telephone and telegraph lines, the article analyses representations of an unstable border at a moment of peak uncertainty. In so doing, it reveals the centrality of architects, engineers, tourist bureaus, artists and cartographers in forging an imaginary geography that correlated with emerging political divisions in post-war Europe. On both sides of the contested border, cartography was utilised to change the way people conceived political geography. Maps, the article argues, both revealed existing mental maps and helped to shape new ones.
Morphodynamics of Barchan‐Barchan Interactions Investigated at the Grain Scale
Authors: Assis W. R. , Franklin E. M.
Published: July 2021

Corridors of size‐selected crescent‐shaped dunes, known as barchans, are commonly found in water, air, and other planetary environments. The growth of barchans results from the interplay between a fluid flow and a granular bed, but their size regulation involves intricate exchanges between different barchans within a field. One size‐regulating mechanism is the binary interaction between nearby dunes, when two dunes exchange mass via the near flow field or by direct contact (collision). In a recent Letter (Assis & Franklin, 2020, https://doi.org/10.1029/2020GL089464), we identified five different patterns arising from binary interactions of subaqueous barchans and proposed classification maps. In this paper, we further inquire into binary exchanges by investigating the motion of individual grains while barchans interact with each other. The experiments were conducted in a water channel where the evolution of pairs of barchans in both aligned and off‐centered configurations was recorded by conventional and high‐speed cameras. Based on image processing, we obtained the morphology of dunes and motion of grains for all interaction patterns. We present the trajectories of individual grains, from which we show the origin and destination of moving grains, and their typical lengths and velocities. We also show that grains from the impacting dune spread with a diffusion‐like component over the target barchan, and we propose a diffusion length. Our results provide new insights into the size‐regulating mechanisms of barchans and barchanoid forms found on Earth and other planets.
Transcriptional response to host chemical cues underpins the expansion of host range in a fungal plant pathogen lineage
Authors: Kusch Stefan , Larrouy Justine , Ibrahim Heba M M , Mounichetty Shantala , Gasset Noémie , Navaud Olivier , Mbengue Malick , Zanchetta Catherine , Lopez-Roques Céline , Donnadieu Cécile , Godiard Laurence , Raffaele Sylvain
Published: July 2021

The host range of parasites is an important factor in assessing the dynamics of disease epidemics. The evolution of pathogens to accommodate new hosts may lead to host range expansion, a process the molecular bases of which are largely enigmatic. The fungus Sclerotinia sclerotiorum has been reported to parasitize more than 400 plant species from diverse eudicot families while its close relative, S. trifoliorum, is restricted to plants from the Fabaceae family. We analyzed S. sclerotiorum global transcriptome reprogramming on hosts from six botanical families and reveal a flexible, host-specific transcriptional program. We generated a chromosome-level genome assembly for S. trifoliorum and found near-complete gene space conservation in two representative strains of broad and narrow host range Sclerotinia species. However, S. trifoliorum showed increased sensitivity to the Brassicaceae defense compound camalexin. Comparative analyses revealed a lack of transcriptional response to camalexin in the S. trifoliorum strain and suggest that regulatory variation in detoxification and effector genes at the population level may associate with the genetic accommodation of Brassicaceae in the Sclerotinia host range. Our work proposes transcriptional plasticity and the co-existence of signatures for generalist and polyspecialist adaptive strategies in the genome of a plant pathogen.
Drosophila attack inhibits hyphal regeneration and defense mechanisms activation for the fungus Trichoderma atroviride
Authors: Atriztán-Hernández Karina , Herrera-Estrella Alfredo
Published: July 2021

The capability to respond to wounding is a process shared by organisms of different kingdoms that can result in the regeneration of whole-body parts or lost structures or organs. Filamentous fungi constitute a rich food source that ensures survival and reproduction of their predators and are therefore continuously exposed to mechanical damage. Nevertheless, our understanding of how fungi respond to wounding and predators is scarce. Fungi like plants and animals respond to injury recognizing Damage- and Microbe-Associated Molecular Patterns (DAMPs/MAMPs) that activate Ca2+ and Mitogen-Activated Protein Kinase dependent signaling for the activation of defense mechanisms. During herbivory, plants, in addition to activating pathways related to injury, activate specific responses to combat their predators. Using a transcriptional approach, we studied the capacity of the filamentous fungus Trichoderma atroviride to activate specific responses to injury and attack by different arthropods. Attack by Drosophila melanogaster inhibited the transcriptional activation of genes required for hyphal regeneration, and the fungal innate immune and chemical defense responses. We also provide mechanistic insight of this inhibition involving components of the D. melanogaster salivary glands that repress the expression of a set of genes and block hyphal regeneration.
The rates of global bacterial and archaeal dispersal
Authors: Louca Stilianos
Published: July 2021

The phylogenetic resolution at which microorganisms display geographic endemism, the rates at which they disperse at global scales, and the role of humans on global microbial dispersal are largely unknown. Answering these questions is necessary for interpreting microbial biogeography, ecology, and macroevolution and for predicting the spread of emerging pathogenic strains. To resolve these questions, I analyzed the geographic and evolutionary relationships between 36,795 bacterial and archaeal (“prokaryotic”) genomes from ∼7000 locations around the world. I find clear signs of continental-scale endemism, including strong correlations between phylogenetic divergence and geographic distance. However, the phylogenetic scale at which endemism generally occurs is extremely small, and most “species” (defined by an average nucleotide identity ≥ 95%) and even closely related strains (average nucleotide identity ≥ 99.9%) are globally distributed. Human-associated lineages display faster dispersal rates than other terrestrial lineages; the average net distance between any two human-associated cell lineages diverging 50 years ago is roughly 580 km. These results suggest that many previously reported global-scale microbial biogeographical patterns are likely the result of recent or current environmental filtering rather than geographic endemism. For human-associated lineages, estimated transition rates between Europe and North America are particularly high, and much higher than for non-human associated terrestrial lineages, highlighting the role that human movement plays in global microbial dispersal. Dispersal was slowest for hot spring- and terrestrial subsurface-associated lineages, indicating that these environments may act as “isolated islands” of microbial evolution.
Single-cell RNA-seq reveals cellular heterogeneity of mouse carotid artery under disturbed flow
Authors: Li Fengchan , Yan Kunmin , Wu Lili , Zheng Zhong , Du Yun , Liu Ziting , Zhao Luyao , Li Wei , Sheng Yulan , Ren Lijie , Tang Chaojun , Zhu Li
Published: July 2021

Disturbed blood flow (d-flow) has been known to induce changes of the cells in the arterial wall, increasing the risk of atherosclerosis. However, the heterogeneity of the vascular cell populations under d-flow remains less understood. To generate d-flow in vivo, partial carotid artery ligation (PCL) was performed. Seven days after ligation, single-cell RNA sequencing of nine left carotid arteries (LCA) from the PCL group (10,262 cells) or control group (14,580 cells) was applied and a single-cell atlas of gene expression was constructed. The integrated analysis identified 15 distinct carotid cell clusters, including 10 d-flow-relevant subpopulations. Among endothelial cells, at least four subpopulations were identified, including Klk8hi ECs, Lrp1hi ECs, Dkk2hi ECs, and Cd36hi ECs. Analysis of GSVA and single-cell trajectories indicated that the previously undescribed Dkk2hi ECs subpopulation was mechanosensitive and potentially transformed from Klk8hi ECs under d-flow. D-flow-induced Spp1hi VSMCs subpopulation that appeared to be endowed with osteoblast differentiation, suggesting a role in arterial stiffness. Among the infiltrating cell subpopulations, Trem2hi Mφ, Birc5hi Mφ, DCs, CD4+ T cells, CXCR6+ T cells, NK cells, and granulocytes were identified under d-flow. Of note, the novel Birc5hi Mφ was identified as a potential contributor to the accumulation of macrophages in atherosclerosis. Finally, Dkk2hi ECs, and Cd36hi ECs were also found in the proatherosclerotic area of the aorta where the d-flow occurs. In conclusion, we presented a comprehensive single-cell atlas of all cells in the carotid artery under d-flow, identified previously unrecognized cell subpopulations and their gene expression signatures, and suggested their specialized functions.
CD40 stimulation as a molecular adjuvant for cancer vaccines and other immunotherapies
Authors: Bullock Timothy N. J.
Published: July 2021

The substantial advances attained by checkpoint blockade immunotherapies have driven an expansion in the approaches used to promote T cell access to the tumor microenvironment to provide targets for checkpoint immunotherapy. Inherent in any T cell response to a tumor antigen is the capacity of dendritic cells to initiate and support such responses. Here, the rationale and early immunobiology of CD40 as a master regulator of dendritic cell activation is reviewed, with further contextualization and appreciation for the role of CD40 stimulation not only in cancer vaccines but also in other contemporary immune-oncology approaches.
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
Authors: Mathieu Mathilde , Névo Nathalie , Jouve Mabel , Valenzuela José Ignacio , Maurin Mathieu , Verweij Frederik J. , Palmulli Roberta , Lankar Danielle , Dingli Florent , Loew Damarys , Rubinstein Eric , Boncompain Gaëlle , Perez Franck , Théry Clotilde
Published: July 2021

Despite their roles in intercellular communications, the different populations of extracellular vesicles (EVs) and their secretion mechanisms are not fully characterized: how and to what extent EVs form as intraluminal vesicles of endocytic compartments (exosomes), or at the plasma membrane (PM) (ectosomes) remains unclear. Here we follow intracellular trafficking of the EV markers CD9 and CD63 from the endoplasmic reticulum to their residency compartment, respectively PM and late endosomes. We observe transient co-localization at both places, before they finally segregate. CD9 and a mutant CD63 stabilized at the PM are more abundantly released in EVs than CD63. Thus, in HeLa cells, ectosomes are more prominent than exosomes. By comparative proteomic analysis and differential response to neutralization of endosomal pH, we identify a few surface proteins likely specific of either exosomes (LAMP1) or ectosomes (BSG, SLC3A2). Our work sets the path for molecular and functional discrimination of exosomes and small ectosomes in any cell type.
Genetic and chemical inhibition of IRF5 suppresses pre-existing mouse lupus-like disease
Authors: Ban Tatsuma , Kikuchi Masako , Sato Go R. , Manabe Akio , Tagata Noriko , Harita Kayo , Nishiyama Akira , Nishimura Kenichi , Yoshimi Ryusuke , Kirino Yohei , Yanai Hideyuki , Matsumoto Yoshiko , Suzuki Shuichi , Hihara Hiroe , Ito Masashi , Tsukahara Kappei , Yoshimatsu Kentaro , Yamamoto Tadashi , Taniguchi Tadatsugu , Nakajima Hideaki , Ito Shuichi , Tamura Tomohiko
Published: July 2021

The transcription factor IRF5 has been implicated as a therapeutic target for the autoimmune disease systemic lupus erythematosus (SLE). However, IRF5 activation status during the disease course and the effects of IRF5 inhibition after disease onset are unclear. Here, we show that SLE patients in both the active and remission phase have aberrant activation of IRF5 and interferon-stimulated genes. Partial inhibition of IRF5 is superior to full inhibition of type I interferon signaling in suppressing disease in a mouse model of SLE, possibly due to the function of IRF5 in oxidative phosphorylation. We further demonstrate that inhibition of IRF5 via conditional Irf5 deletion and a newly developed small-molecule inhibitor of IRF5 after disease onset suppresses disease progression and is effective for maintenance of remission in mice. These results suggest that IRF5 inhibition might overcome the limitations of current SLE therapies, thus promoting drug discovery research on IRF5 inhibitors.