bluuebunny/crossref_metadata_2025 huggingface dataset:
Predictors for large vessel recanalization before stroke thrombectomy: the HALT score
Authors: Colasurdo Marco , Chen Huanwen , Schrier Chad , Khalid Mazhar , Khunte Mihir , Miller Timothy R , Cherian Jacob , Malhotra Ajay , Gandhi Dheeraj
Published: April 2023

Background

Large vessel recanalization (LVR) before endovascular therapy (EVT) for acute large vessel ischemic strokes is a poorly understood phenomenon. Better understanding of predictors for LVR is important for optimizing stroke triage and patient selection for bridging thrombolysis.

Methods

In this retrospective cohort study, consecutive patients presenting to a comprehensive stroke center for EVT treatment were identified from 2018 to 2022. Demographic information, clinical characteristics, intravenous thrombolysis (IVT) use, and LVR before EVT were recorded. Factors independently associated with different rates of LVR were identified, and a prediction model for LVR was constructed.

Results

640 patients were identified. 57 (8.9%) patients had LVR before EVT. A minority (36.4%) of LVR patients had significant improvements in National Institutes of Health Stroke Scale. Independent predictors for LVR were identified and used to construct the 8-point HALT score: hyperlipidemia (1 point), atrial fibrillation (1 point), location of vascular occlusion (internal carotid: 0 points, M1: 1 point, M2: 2 points, vertebral/basilar: 3 points), and thrombolysis at least 1.5 hours before angiography (3 points). The HALT score had an area under the receiver-operating curve (AUC) of 0.85 (95% CI 0.81 to 0.90, P<0.001) for predicting LVR. LVR before EVT occurred in only 1 of 302 patients (0.3%) with low (0–2) HALT scores.

Conclusions

IVT at least 1.5 hours before angiography, site of vascular occlusion, atrial fibrillation, and hyperlipidemia are independent predictors for LVR. The 8-point HALT score proposed in this study may be a valuable tool for predicting LVR before EVT.
Innovative approach to medical leadership and management development: clinician secondment to a management consulting firm
Authors: Papalia Francesco , Fung Kenneth , Chen Yang , Thornton George D , Geatches Nick , Cousins Frances , Kirkham Karen , Westwood Mark
Published: April 2023

Background and aim

Opportunities to participate in leadership and management with protected time can be limited for clinical trainees. The aim of this fellowship was to gain experience of gold standard healthcare management by becoming part of multidisciplinary teams working to deliver transformational change in the National Health Service (NHS).

Methods

A 6-month pilot fellowship, structured as an Out of Programme Experience was created for two registrars to be seconded to the healthcare division of Deloitte, a leading professional services firm. Competitive selection was jointly administered by the Director of Medical Education at St Bartholomew’s Hospital and Deloitte.

Results

The successful candidates worked on service-led and digital transformation projects, interfacing with senior NHS executives and directors. Trainees gained direct experience and understanding of high-level decision making in the NHS, tackling complex service delivery problems and the practical realities of delivering change within a constrained budget. One impact of this pilot has been completion of a business case to scale up the fellowship into an established programme that can allow other trainees to apply.

Conclusion

This innovative fellowship has allowed interested trainees an opportunity to broaden the relevant skills and experience in leadership and management required in specialty training curriculum with real-life application in the NHS.
On the factors affecting migration and straying in brown trout (Salmo trutta)
Authors: Källo Kristi , Birnie-Gauvin Kim , Baktoft Henrik , Aarestrup Kim
Published: April 2023

Anadromous brown trout display a continuum of life-history strategies with high intraspecific variability across their distribution range. While many of these strategies have been widely studied, some have received considerably less attention. In this study, we investigated intraspecific differences in two overlooked life-history strategies—outmigration phenology (spring vs. autumn migrants) and the prevalence of straying—in brown trout originating from four rivers flowing into the same fjord system. Juvenile brown trout ( Salmo trutta) were tagged with passive integrated transponder (PIT) tags in their natal river, and were subsequently followed during their outmigration and subsequent return to freshwater. We found that patterns of outmigration (spring vs. autumn migrant, day of season) and return to freshwater (return likelihood, strayers vs. nonstrayers) differed between rivers, as did the factors (length, condition) affecting these outcomes. We also found evidence that outmigration phenology was linked to the likelihood of returning in a river-specific way. Our findings suggest that the costs and benefits of autumn migration and straying differ depending on an individual's home river.
Dynamic Analysis of FGM Plates with Variable Delamination Parameters by the Chebyshev–Ritz Method
Authors: Wang J. F. , Cao G. , Song Z. W. , Lai S. K.
Published: April 2023

Delamination is one of the failure modes of functionally graded materials (FGM), resulting in the critical change of vibration characteristics. The Chebyshev polynomials are commonly used as admissible functions to improve the computational efficiency of numerical algorithms and avoid the occurrence of ill-conditioned problems. This paper extends the Chebyshev–Ritz method to the free vibration analysis of delaminated FGM plates, in which the material variation through the plate thickness follows the exponential-law distribution. A plane crack that is considered to be perpendicular to the thickness direction penetrates through the width direction. Based on the region approach, the analysis of FGM plates with a mid-plane delamination is divided into four sub-regions. The kinetic energy and potential energy of each sub-region are derived by the thin plate theory and von Kármán nonlinear strain–displacement relation. The modal functions of the displacement fields of FGM plates can be constructed in accordance with the displacement continuity conditions of the delamination interface and the boundary conditions of such plates. The effects of asymmetric material distribution, delamination length ratio, Young’s modulus ratio, and boundary support on the vibration behavior of FGM plates are investigated. This semi-analytical study provides a reasonable theoretical basis for the behavior prediction and delamination identification of composite structures.
Numerical Analysis and Experimental Study on Seismic Coupling Response of Pipe-Soil System Under Bidirectional Seismic Excitation
Authors: Dai Jianbo , Wang Hao , Hu Chengtao , Zhao Zewen , Ma Jing
Published: April 2023

The seismic response of buried pipeline is significantly affected by the soil around the pipeline. In this study, a numerical analysis model of the pipeline and the soil around the pipeline is built, and the finite element numerical analysis of its seismic response law is conducted to address the problem of pipeline soil seismic coupling response. A shaking table test of the seismic response of buried pipeline under two-way seismic excitation is performed based on the developed two-way layered shear continuum model soil box. The peak strain response at the middle section of the pipeline grows fastest, the peak strain of the pipeline under non-uniform seismic excitation constantly exceeds that under uniform excitation, and the axial strain of the pipeline exhibits more sensitivity to earthquake than the bending strain, as indicated by the results of numerical analysis and shaking table test. The peak value of soil acceleration response under non-uniform excitation exceeds under uniform excitation. The displacement of soil increases with the height. The pipeline soil interaction becomes more intense under non-uniform excitation, and the relative movement between pipeline and soil becomes more significant. The seismic excitation more significantly affects the axial displacement of soil. The peak value of soil acceleration response varies from larger along the pipeline to larger along the axis with the increase of the loading grade. A slight difference exists between the peak value of axial and transverse acceleration response of the pipeline. The peak value of pipeline acceleration response declines in the axial direction than the peak value of soil acceleration response, whereas it rises in the transverse direction than the peak value of soil acceleration response, especially under non-uniform excitation. As revealed by the above results, the effect of bidirectional non-uniform seismic excitation on the lateral pipeline soil interaction is enhanced, and soil is more seriously damaged.
A New Method for Analyzing Dynamic Characteristics of Generalized Planar Frame Structures Under Complex Conditions
Authors: Wu Zonghuan , Ma Qianying , Mei Yang , Sun Zheng , Wang Yabo , Li Bingbing
Published: April 2023

This paper presents a novel method for analyzing the vibration characteristics of planar frame structure composed of straight beams with arbitrary shapes. The method is based on the first-order shear theory, which takes into account the shear deformation and cross-sectional rotational inertia of the beams. On the one hand, an improved Fourier series method (IFSM) is employed to approximate the displacement function of the straight beam, and the displacements are determined by using the Rayleigh–Ritz method. On the other hand, the displacements of the curved beam are obtained by using the finite element method (FEM), which are then combined with the IFSM for the analysis of coupled nodes of straight and curved beams. This results in a computational method for calculating the vibration characteristics of complex planar frame structures with concentrated masses, straight and curved beams, arbitrary boundary conditions, and any connection form of nodes. The applicability of the proposed method is demonstrated through numerical examples, including planar frame structure with arbitrary shapes composed of straight beams, different cross-sectional shapes, and combinations of beam with various cross-sectional shapes, as well as planar frame structures with straight and curved beams. The results of the proposed method are validated by comparing them with those obtained from literature or finite element software ABAQUS. The numerical examples show that the proposed method combining the IFSM with FEM has the advantages of high accuracy, good convergence, and strong applicability for calculating the vibration characteristics of planar frame structures.
A Proposed Nonaligned Double Gate Junction FET Device and its Performance Improvement Using High-k Gate Oxide Material
Authors: Sinha Arun Kumar , Naik Banoth Vasu
Published: April 2023

This paper presents the effect of dielectric materials i.e., hafnium dioxide (HfO2, high-[Formula: see text] and silicon dioxide (SiO2, low-[Formula: see text], as gate-oxide material for the nonaligned double gate junction [Formula: see text]-channel field effect transistor (NADG-NFET). The NADGNFET device proposed in this work lowers the second order effects and improves the transistor linear performance at radio frequency. The device response with gate-oxide material is investigated by using two dielectric materials on the obtained current–voltage characteristics, intrinsic gain, and linearity parameter. The device simulations were done using a 2D-sentaurus TCAD tool. The results were examined in terms of DIBL (drain-induced barrier lowering), SS (subthreshold swing), [Formula: see text] current, [Formula: see text] ratio, the Intrinsic gain ([Formula: see text], and intermodulation distortion power-3 (IMD3) parameter. It has been found that high-[Formula: see text] dielectric decreases the DIBL by 40%, improves the [Formula: see text] ratio by 8 times, and also improves the intrinsic gain by 38% compared to low-[Formula: see text] dielectric material. However, the high frequency parameter result was better with low-[Formula: see text] dielectric material. This gives a trade-off in the device applications. The IMD3 plot shows that using the two gate-oxide material will give the same performance to the radio frequency (RF) signal.
Hopf bifurcation in a delayed prey–predator model with prey refuge involving fear effect
Authors: Parwaliya Ankit , Singh Anuraj , Kumar Ajay
Published: April 2023

This work investigates a prey–predator model featuring a Holling-type II functional response, in which the fear effect of predation on the prey species, as well as prey refuge, are considered. Specifically, the model assumes that the growth rate of the prey population decreases as a result of the fear of predators. Moreover, the detection of the predator by the prey species is subject to a delay known as the fear response delay, which is incorporated into the model. The paper establishes the preliminary conditions for the solution of the delayed model, including positivity, boundedness and permanence. The paper discusses the existence and stability of equilibrium points in the model. In particular, the paper considers the discrete delay as a bifurcation parameter, demonstrating that the system undergoes Hopf bifurcation at a critical value of the delay parameter. The direction and stability of periodic solutions are determined using central manifold and normal form theory. Additionally, the global stability of the model is established at axial and positive equilibrium points. An extensive numerical simulation is presented to validate the analytical findings, including the continuation of the equilibrium branch for positive equilibrium points.
TEMPERATURE AND ENERGY SECURITY: WILL FOREST BIOMASS HELP IN THE FUTURE?
Authors: FAVERO ALICE , YOO JONGHYUN , DAIGNEAULT ADAM , BAKER JUSTIN
Published: April 2023

Despite the numerous technical, logistical, and policy challenges associated with the use of bioenergy to mitigate climate change, the latest IPCC report identifies bioenergy as a high-value and large-scale mitigation option to support the transition to a cleaner energy system. This paper links a climate-economic-energy model and a land model to measure the net mitigation effect of using forest biomass for electricity generation and corresponding implications on global temperature. Through the soft-link, the energy model provides to the land model the cost-effective regional consumption of forest biomass under nine carbon price scenarios and measures the effects of its use on fossil fuel emissions and carbon sequestered in carbon capture and storage (CCS). The land model provides the dynamic supply of forest biomass and measures the change in land management/use under each demand scenario and corresponding changes in carbon sequestered in forests. Results suggest that forest biomass should be part of global mitigation efforts despite the expected small share of electricity sourced from it. The net climate benefits of forest biomass energy vary across scenarios and temporally — in most scenarios increased biomass demand results in near term reductions in global forest carbon stocks, but at carbon prices starting at $40/tCO2e or greater, results show positive net sequestration by 2030. This increased sequestration, coupled with energy emissions displacement and bioenergy with carbon capture and storage (BECCS) implies substantial long-term mitigation potential for forest biomass energy. Our results suggest that high forest biomass demand pathways could also help reduce the magnitude of future temperature growth. Further, we explore the regional effects on energy security of using forest biomass. Results show that its use can have potential large effects on trade dynamics and regional energy security issues, with 4 of the 17 global regions found to be net exporters of forest biomass.
Black Holes have Intrinsic Scalar Curvature
Authors: Morley P. D.
Published: April 2023

The scalar curvature R is invariant under isometric symmetries (distance invariance) associated with metric spaces. Gravitational Riemannian manifolds are metric spaces. For Minkowski Space, the distance invariant is [Formula: see text], where x, y are arbitrary 4-vectors. Thus the isometry symmetry associated with Minkowski Space is the Poincaré Group. The Standard Model Lagrangian density [Formula: see text] is also invariant under the Poincaré Group, so for Minkowski Space, the scalar curvature and the Standard Model Lagrangian density are proportional to each other. We show that this proportionality extends to general gravitational Riemannian manifolds, not just for Minkowski Space. This predicts that Black Holes have non-zero scalar curvatures [Formula: see text]. For Schwarzschild Black Holes, [Formula: see text] is predicted to be [Formula: see text], where [Formula: see text] is the Schwarzschild radius. The existence of [Formula: see text] means that Black Holes cannot evaporate.
Hypergraph Association Weakly Supervised Crowd Counting
Authors: Li Bo , Zhang Yong , Zhang Chengyang , Piao Xinglin , Yin Baocai
Published: April 2023

Weakly supervised crowd counting involves the regression of the number of individuals present in an image, using only the total number as the label. However, this task is plagued by two primary challenges: the large variation of head size and uneven distribution of crowd density. To address these issues, we propose a novel Hypergraph Association Crowd Counting (HACC) framework. Our approach consists of a new multi-scale dilated pyramid module that can efficiently handle the large variation of head size. Further, we propose a novel hypergraph association module to solve the problem of uneven distribution of crowd density by encoding higher-order associations among features, which opens a new direction to solve this problem. Experimental results on multiple datasets demonstrate that our HACC model achieves new state-of-the-art results.
Systems Immunology Approaches to Metabolism
Authors: Mogilenko Denis A. , Sergushichev Alexey , Artyomov Maxim N.
Published: April 2023

Over the last decade, immunometabolism has emerged as a novel interdisciplinary field of research and yielded significant fundamental insights into the regulation of immune responses. Multiple classical approaches to interrogate immunometabolism, including bulk metabolic profiling and analysis of metabolic regulator expression, paved the way to appreciating the physiological complexity of immunometabolic regulation in vivo. Studying immunometabolism at the systems level raised the need to transition towards the next-generation technology for metabolic profiling and analysis. Spatially resolved metabolic imaging and computational algorithms for multi-modal data integration are new approaches to connecting metabolism and immunity. In this review, we discuss recent studies that highlight the complex physiological interplay between immune responses and metabolism and give an overview of technological developments that bear the promise of capturing this complexity most directly and comprehensively.
Recent Developments in Causal Inference and Machine Learning
Authors: Brand Jennie E. , Zhou Xiang , Xie Yu
Published: April 2023

This article reviews recent advances in causal inference relevant to sociology. We focus on a selective subset of contributions aligning with four broad topics: causal effect identification and estimation in general, causal effect heterogeneity, causal effect mediation, and temporal and spatial interference. We describe how machine learning, as an estimation strategy, can be effectively combined with causal inference, which has been traditionally concerned with identification. The incorporation of machine learning in causal inference enables researchers to better address potential biases in estimating causal effects and uncover heterogeneous causal effects. Uncovering sources of effect heterogeneity is key for generalizing to populations beyond those under study. While sociology has long emphasized the importance of causal mechanisms, historical and life-cycle variation, and social contexts involving network interactions, recent conceptual and computational advances facilitate more principled estimation of causal effects under these settings. We encourage sociologists to incorporate these insights into their empirical research.
Cultural Objects, Material Culture, and Materiality
Authors: McDonnell Terence E.
Published: April 2023

The study of cultural objects and their materiality has moved to the center of cultural sociology. This review synthesizes the work of this third wave of cultural sociology, demonstrating how insights from the study of cultural objects and their mechanisms of meaning-making deepen our theories of culture in action, culture and cognition, and the production and reception of culture. After placing this third wave in the historical context of cultural sociology, this review clarifies three concepts: cultural objects, material culture, and materiality. This review then makes a series of interventions around meaning-making and action based on insights from scholarship on cultural objects and materiality. First, it advocates attention to qualities in addition to symbols. Then it examines how object affordances constrain and enable meaning and use and how objects have material agency. Then the role of cultural objects in stabilizing and destabilizing meaning and social arrangements is discussed. Finally, cultural power—whether and how cultural objects shape belief and behavior—is considered through the orienting concepts of figure and ground.
Adaptive responses in uteroplacental metabolism and fetoplacental nutrient shuttling and sensing during placental insufficiency
Authors: Kyllo Hannah M. , Wang Dong , Lorca Ramón A. , Julian Colleen G. , Moore Lorna G. , Wilkening Randall B. , Rozance Paul J. , Brown Laura D. , Wesolowski Stephanie R.
Published: April 2023

These results suggest increased placental utilization of pyruvate from the fetus, without higher maternal glucose uptake, and lower amino acid shuttling in the placental insufficiency-induced intrauterine growth restriction (PI-IUGR) placenta. AMPK activation was associated with oxidative stress and PDH activity, supporting a putative link between oxidative stress, AMPK, and pyruvate utilization. These differences in fetoplacental nutrient sensing and shuttling may represent adaptive strategies enabling the placenta to maintain oxidative metabolism at the expense of fetal growth.
Visuo-proprioceptive recalibration and the sensorimotor map
Authors: Block Hannah J. , Liu Yang
Published: April 2023

If perceived hand position changes, we might assume this affects the sensorimotor map and, in turn, reaches made with that hand. However, there is evidence for separate body representations involved in perception versus action. After a cross-sensory conflict that results in proprioceptive recalibration in the forward direction, participants made shorter reaches as predicted, but only briefly. This suggests perceptual recalibration does affect the sensorimotor map used to plan reaches, but the interaction may be short-lived.
A Novel Hybrid Model for the Evaluation of Industry 4.0 Technologies’ Applicability in Logistics Centers
Authors: Miškić Smiljka , Tadić Snežana , Stević Željko , Krstić Mladen , Roso Violeta
Published: April 2023

The application of Industry 4.0 (I4.0) in the field of logistics leads to the emergence and development of the concept of logistics 4.0. Many I4.0 technologies have been applied in the field of logistics. The goal of this research is to analyze the applicability of nine key I4.0 technologies in logistics centers (LC). For this purpose, an integrated MEREC (MEthod based on the Removal Effects of Criteria)—fuzzy MARCOS (Measurement of Alternatives and Ranking according to COmpromise Solution) model was developed. The applicability of nine I4.0 technologies was evaluated based on 15 subcriteria within three main groups of criteria, namely, technological, social and political, and economic and operative. Using the MEREC method, the weight values of the criteria and subcriteria were determined, while the technologies were ranked using the fuzzy MARCOS method. Based on the results obtained by applying this integrated MCDM (multicriteria decision-making) model, CC was identified as the best alternative, i.e., the technology that is most applicable in logistics centers, followed by IoT and big data. An analysis of the sensitivity of the obtained results to the change in the importance of the criteria was carried out, which shows certain changes in the ranking when the importance of the most important criterion changes.
Plant-Mediated Synthesis of Ni-Doped CuO and Fe2O3 Nanocomposite for Photodegradation of Methylene Blue Dye
Authors: Debele Ermias Tafa , Desissa Temesgen Debelo , Zelekew Osman Ahmed , Bakare Fetene Fufa , Feyisa Gebisa Bekele , Wondimu Tadele Hunde
Published: April 2023

Cu1–xNixO/Fe2O3 (with x = 0.01, 0.02, 0.03, 0.04 wt%) were synthesized by plant extraction technique. The absorbance and degradation performance of Ni-doped CuO and Fe2O3 nanocomposite against methylene blue (MB) was analyzed, and the 2% Ni-doped CuO yielded an optimum result, and the optical bandgap of CuO, 2NCO, Fe2O3, and (60%) 2NCO/40% Fe2O3 was found to be 1.88, 1.73, 2.1, and 1.80 eV, respectively. Hence, the 2% Ni-doped CuO (2NCO) was further used for the establishment of a composite with Fe2O3. The lowest composition of the oxide composite was (1−x) 2NCO/(x) Fe2O3 (x = 0.1, 0.2, 0.3, 0.4, and 0.5). The degradation performance of those oxide composites was determined against (MB) with the nominal composition of sample (60%) 2NCO/40% Fe2O3 resulted in an optimum degradation of MB with a percentage of 94% at 120 min. The recyclability takes a look at was performed for five cycles at the start of 94%; after five cycles, the sample remained stable at 120 min. Therefore, 2NCO/40% Fe2O3 composite is going to be the selection material for waste product treatment.
Effectiveness of Polyclonal Antibody Immunoconjugate Treatment with Propamidine Isethionate for Amoebic Keratitis in Golden Hamsters
Authors: Ávila-Blanco Manuel Enrique , Aguilera-Martínez Sandra Lizbeth , Ventura-Juarez Javier , Pérez-Serrano Jorge , Casillas-Casillas Elizabeth , Barba-Gallardo Luis Fernando
Published: April 2023

Acanthamoeba griffini is known to cause amoebic keratitis (AK); its main causes are inadequate hygiene when contact lenses are handled and/or its prolonged use at night, as well as the use of contact lenses during underwater activities. The most used treatment for AK is the combination of propamidine isethionate combined with polyhexamethylene biguanide, which disrupts the cytoplasmic membrane, and damages cellular components and respiratory enzymes. We proposed an immunoconjugate treatment obtained from Acanthamoeba immunized rabbit serum combined with propamidine isethionate; the corneas of hamsters inoculated with A. griffini (MYP2004) were treated with the combined, at 1, 2, and 3 weeks. Propamidine isethionate is frequently used for AK treatment, in vivo study we are found IL-1β and IL-10 expression and caspase 3 activity is significantly increased with respect to the group that was inoculated with the amoeba without receiving any treatment, suggesting that it may be an effect of the toxicity of this drug on the corneal tissue. Application of the immunoconjugate showed enhanced amoebicidal and anti-inflammatory activities, with comparison to propamidine isethionate only. The aim of this study is to evaluate the effect of the immunoconjugate of propamidine isethionate and polyclonal antibodies as a treatment of AK in golden hamsters (Mesocricetus auratus).
3-D Printed Circularly Polarized Open-Ended Waveguide Antenna Array Based on an In-Built Antipodal Exponential Groove Polarizer
Authors: Ma Zi Long , Zhang Chu Wei
Published: April 2023

This paper presents a novel 3-D-printed circularly polarized (CP) open-ended waveguide (OEW) antenna array. It aims to provide a lightweight scheme for OEW array design and concurrently address the high fabrication complexity problem of conventional OEW arrays. To realize these, the proposed design adopts dielectric 3-D printing technology to build the main body of the array and employs electroplating to form the waveguide walls. To attain CP radiation, an in-built antipodal exponential groove polarizer is proposed and applied to a square waveguide. With the perturbation of the grooves, degenerate modes

T

E

10

and

T

E

01

can be excited simultaneously, and their differential phase can be 90 degrees, resulting in CP radiation. Moreover, a novel matching cube based on effective medium theory (EMT) is proposed for the antenna to mitigate the reflections at the waveguide aperture and therefore realize good impedance matching. To validate the design idea, prototypes for a single element and a 2

×

2 array were fabricated and measured. Experimental results show that their weights are 15 and 76 grams, and their axial ratio bandwidths (ARBWs) are 16% (5.45-6.4 GHz) and 11.1% (5.52-6.17 GHz), respectively.
A Comparative Evaluation of the Apically Extruded Debris from Root Canals Prepared by R-Motion NiTi File System
Authors: Al-Saffar Farah B. , Al-Gharrawi Hikmet A.
Published: April 2023

Background. Apically extruded debris (AED) is an inherent concern during root canal treatment for both endodontists and general practitioners. The present study investigates the AED of the novel R-Motion single-file reciprocating system compared to standard single reciprocating and multifile rotary systems. Materials and Methods. Fifty-six moderately curved palatal roots of upper maxillary first molars were selected for the present study. The samples were then divided randomly into four groups (n = 14)— Group I: R-motion (RM), Group II: WaveOne Gold (WOG), Group III: ProTaper Next (PTN), and Group IV: HyFlex EDM (HFEDM). The researcher has modified Myers and Montgomery’s method to simulate human body temperature. Vials were used to collect debris and weighted using a 0.00001 sensitive balance before and after instrumentation. The instrumentation of all experimented groups was done at 37°C, terminated at master apical file #25. An auto syringe with a side vented needle was used to deliver 8 ml of deionized water for irrigation of each sample during preparation. Vials were stored in a dry sealed desiccator which contained CaSO4 crystals, for 24 hr before weighing. The weight of the collected debris was obtained by subtracting the preinstrumentation weight from the postinstrumentation weight for each vial. The Kruskal–Wallis and Mann–Whitney U tests were performed to analyse the statistical difference in the amount of debris between the tested groups at a 0.05 significance level. Results. The RM system produced less debris extrusion than all tested groups, with a significant difference between the former and the WOG and the PTN systems. However, WOG, PTN, and HFEDM showed no statistically significant difference in the amount of AED. Conclusion. All tested groups produced apical debris in different amounts. The RM produced substantially less AED than WOG and PTN. Meanwhile, WOG, PTN, and HFEDM caused a comparable amount of AED.
Extraction and Characterization of Type I Collagen from Parrotfish (Scarus sordidus Forsskål, 1775) Scale solubilized with the Aid of Acetic Acid and Pepsin
Authors: Jaziri Abdul Aziz , Shapawi Rossita , Mokhtar Ruzaidi Azli Mohd , Noordin Wan Norhana Md. , Huda Nurul
Published: April 2023

Waste from marine fish processing is an important source of valuable products. Fish collagen is considered a alternative biomaterial due to its excellent properties, and it is widely used for industrial purposes. Thus, this present study aimed to characterize acid and pepsin-soluble collagens from the waste of parrotfish (Scarus sordidus Forsskål, 1775) scales. The yields (p > 0.05) of acid-soluble collagen (ASC-PFS) and pepsin-soluble collagen (PSC-PFS) were 1.17 g/100 g and 1.00 g/100 g, respectively. Both collagen samples were categorized as type I owing to the presence of two alpha chain subunits (α1 and α1) after being confirmed by a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Under the fourier transform infrared (FTIR) test, the triple helical structure of type I collagens from the ASC-PFS and PSC-PFS was maintained. Moreover, the study of UV visible spectra and X-ray diffraction (XRD) showed the similarity of collagens derived from different fish species, and the thermostability (Tmax) evaluation of all extracted collagens was in the range of 36.22–37.78°C, and their values were comparable to previous research on the fish scale collagens. The effect of various pH and sodium chloride (NaCl) treatments on solubility exhibited that the ASC-PFS and PSC-PFS were highly soluble in an acidic condition (pH < 5.0) and low concentration of sodium chloride (<30 g/L). Taken together, collagens extracted from parrotfish scale waste can be an alternative source for industries.
Preparation of One-Emission Nitrogen-Fluorine-Doped Carbon Quantum Dots and Their Applications in Environmental Water Samples and Living Cells for ClO− Detection and Imaging
Authors: Zhang Qianchun , Du Haijiang , Xie Siqi , Tian Fengling , Long Xixi , Liu Shan , Wu Yun
Published: April 2023

Hypochlorite (ClO−) has received extensive attention owing to its significant roles in the immune defense and pathogenesis of numerous diseases. However, excessive or misplaced production of ClO− may pose certain diseases. Thus, to determine its biological functions in depth, ClO− should be tested in biosystems. In this study, a facile, one-pot synthesis of nitrogen-fluorine-doped carbon quantum dots (N, F-CDs) was developed using ammonium citrate tribasic, L-alanine, and ammonium fluoride as raw materials under hydrothermal conditions. The prepared N, F-CDs demonstrate not only strong blue fluorescence emission with a high fluorescence quantum yield (26.3%) but also a small particle size of approximately 2.9 nm, as well as excellent water solubility and biocompatibility. Meanwhile, the as-prepared N, F-CDs exhibit good performance in the highly selective and sensitive detection of ClO−. Thus, a wide concentration response range of 0–600 μM with a low limit of detection (0.75 μM) was favorably obtained for the N, F-CDs. Based on the excellent fluorescence stability, excellent water solubility, and low cell toxicity, the practicality and viability of the fluorescent composites were also successfully verified via detecting ClO− in water samples and living RAW 264.7 cells. The proposed probe is expected to provide a new approach for detecting ClO− in other organelles.
[Retracted] The Effect of Hyperlipidemia on Peri‐implant Health: A Clinical and Radiographical Prospective Study
Authors: De Angelis Paolo , Rella Edoardo , Manicone Paolo Francesco , Gasparini Giulio , Giovannini Valerio , Liguori Margherita Giorgia , Camodeca Francesca , De Rosa Giuseppe , Cavalcanti Camilla , D’Addona Antonio
Published: April 2023

High levels of cholesterol and triglycerides may have a negative effect on the immune system and bone health, leading to lower bone mineral density, an increased risk of osteoporosis, and bone fractures, and could therefore also be related to a significant worsening of peri‐implant health. The purpose of the following study was to evaluate whether the altered lipid profile in patients who undergo implant insertion surgery represents a prognostic factor capable of influencing clinical outcomes. This prospective observational study was conducted on 93 subjects; patients were required to have taken blood tests to obtain triglycerides (TG), total cholesterol, low‐density lipoprotein (LDL), and high‐density lipoprotein (HDL) levels prior to the surgical procedure to classify them according to current American Heart Association guidelines. The outcomes considered were marginal bone loss (MBL) 3 years after implant placement, full‐mouth plaque score (FMPS), and full‐mouth bleeding score (FMBS) 3 years after surgery. A statistically significant correlation was found between hypertriglyceridemia and MBL as well as between total cholesterol and MBL. There is no statistically significant correlation between the variables analyzed and the secondary outcomes 3 years after implant placement. Peri‐implant marginal bone loss may be influenced by hyperlipidemia. However, further studies are needed, with larger samples and more extensive follow‐ups, to confirm these results.
[Retracted] Analysis of VLF Wave Field Components and Characteristics Based on Finite Element Time‐Domain Method
Authors: Wang Qile , Zhu Hanhao , Chai Zhigang , Cui Zhiqiang , Wang Yafen
Published: April 2023

Most traditional sound field calculation methods regard the seabed as the horizontal stratified liquid sea bottom and conduct simulation analysis based on the frequency domain. Hence, the generality of the above research methods is limited to varying degrees. To accurately clarify the propagation characteristics and mechanism of very low‐frequency (VLF, ≤100 Hz) sound waves in the shallow sea, a numerical calculation model is established using the finite element time‐domain method (FETD) based on the three‐dimensional cylindrical coordinate system. Using this model, the effects of sea‐bottom topographies and geoacoustic parameters on the composition and characteristics of VLF sound fields in the shallow sea and their corresponding mechanism are investigated through the comparative analysis of various numerical simulation examples. The simulation results demonstrate that the low‐frequency sound field in the full waveguide of the shallow sea is composed of normal mode waves in the seawater layer, Scholte waves at the liquid‐solid interface, and elastic waves at the sea bottom. Compared with the soft sea bottom, which has a more negligible elastic impedance, the hard sea bottom is more conducive to the long‐distance propagation of normal mode waves and the excitation of Scholte waves. The Scholte waves on the hard sea bottom are significantly stronger than those on the soft sea bottom. Compared with the horizontal sea bottom, the uphill topography enhances the sound energy leakage to the sea bottom. It is more favorable to receive Scholte waves at shallow depths, whereas the influence laws of downhill topography are the opposite.
Data from BLEACH&STAIN 15-marker Multiplexed Imaging in 3,098 Human Carcinomas Reveals Six Major PD-L1–driven Immune Phenotypes with Distinct Spatial Orchestration
Authors: Bady Elena , Möller Katharina , Mandelkow Tim , Raedler Jonas B. , Yang Cheng , Ebner Julia , Lurati Magalie C.J. , Simon Ronald , Vettorazzi Eik , Büscheck Franziska , Luebke Andreas M. , Dum David , Menz Anne , Sauter Guido , Höflmayer Doris , Weidemann Sören , Fraune Christoph , Uhlig Ria , Bernreuther Christian , Jacobsen Frank , Clauditz Till S. , Wilczak Waldemar , Burandt Eike , Steurer Stefan , Minner Sarah , Lennartz Maximilian , Blessin Niclas C.
Published: April 2023

Abstract
Multiplex fluorescence IHC (mfIHC) approaches were yet either limited to six markers or limited to a small tissue size that hampers translational studies on large tissue microarray cohorts. Here we have developed a BLEACH&STAIN mfIHC method that enabled the simultaneous analysis of 15 biomarkers (PD-L1, PD-1, CTLA-4, panCK, CD68, CD163, CD11c, iNOS, CD3, CD8, CD4, FOXP3, CD20, Ki67, and CD31) in 3,098 tumor samples from 44 different carcinoma entities within one week. To facilitate automated immune checkpoint quantification on tumor and immune cells and study its spatial interplay an artificial intelligence–based framework incorporating 17 different deep-learning systems was established. Unsupervised clustering showed that the three PD-L1 phenotypes (PD-L1+ tumor and immune cells, PD-L1+ immune cells, PD-L1−) were either inflamed or noninflamed. In inflamed PD-L1+patients, spatial analysis revealed that an elevated level of intratumoral M2 macrophages as well as CD11c+ dendritic cell (DC) infiltration (P < 0.001 each) was associated with a high CD3+ CD4± CD8± FOXP3± T-cell exclusion and a high PD-1 expression on T cells (P < 0.001 each). In breast cancer, the PD-L1 fluorescence intensity on tumor cells showed a significantly higher predictive performance for overall survival (OS; AUC, 0.72, P < 0.001) compared with the commonly used percentage of PD-L1+ tumor cells (AUC, 0.54). In conclusion, our deep-learning–based BLEACH&STAIN framework facilitates rapid and comprehensive assessment of more than 60 spatially orchestrated immune cell subpopulations and its prognostic relevance.
Implications:
The development of an easy-to-use high-throughput 15+1 multiplex fluorescence approach facilitates the in-depth understanding of the immune tumor microenvironment (TME) and enables to study the prognostic relevance of more than 130 immune cell subpopulations.
Supplementary Table S2 from Mitochondrial Metabolism Drives Low-density Lipoprotein-induced Breast Cancer Cell Migration
Authors: Nóbrega-Pereira Sandrina , Santos Francisco , Oliveira Santos Miguel , Serafim Teresa L. , Lopes Ana Patrícia , Coutinho Diogo , Carvalho Filipa S. , Domingues Rosário M. , Domingues Pedro , Bernardes de Jesus Bruno , Morais Vanessa A. , Dias Sérgio
Published: April 2023

Fatty acid profile in relative content (%). Qualitative analysis was calculated by dividing each raw area by the sum of total raw areas. Dash (‘-‘) represents lipid species that were not identified in the sample. Legend: CTR (control), ETO (etomoxir, 100 µM).
Figure S7 from Dissecting the origin of heterogeneity in uterine and ovarian carcinosarcomas
Authors: Sertier Anne-Sophie , Ferrari Anthony , Pommier Roxane M. , Treilleux Isabelle , Boyault Sandrine , Devouassoux-Shisheboran Mojgan , Kielbassa Janice , Thomas Emilie , Tonon Laurie , Le Texier Vincent , Charreton Amandine , Morel Anne-Pierre , Floquet Anne , Joly Florence , Berton-Rigaud Dominique , Ferron Gwenaël , Arnould Laurent , Croce Sabrina , Bataillon Guillaume , Saintigny Pierre , Mery-Lamarche Eliane , Sagan Christine , Senaratne Aruni P. , Gut Ivo G. , Calvo Fabien , Viari Alain , Ouzounova Maria , Ray-Coquard Isabelle , Puisieux Alain
Published: April 2023

Alteration of chromatin remodeling genes in uterine and ovarian CS.
Figure S15 from Dissecting the origin of heterogeneity in uterine and ovarian carcinosarcomas
Authors: Sertier Anne-Sophie , Ferrari Anthony , Pommier Roxane M. , Treilleux Isabelle , Boyault Sandrine , Devouassoux-Shisheboran Mojgan , Kielbassa Janice , Thomas Emilie , Tonon Laurie , Le Texier Vincent , Charreton Amandine , Morel Anne-Pierre , Floquet Anne , Joly Florence , Berton-Rigaud Dominique , Ferron Gwenaël , Arnould Laurent , Croce Sabrina , Bataillon Guillaume , Saintigny Pierre , Mery-Lamarche Eliane , Sagan Christine , Senaratne Aruni P. , Gut Ivo G. , Calvo Fabien , Viari Alain , Ouzounova Maria , Ray-Coquard Isabelle , Puisieux Alain
Published: April 2023

Complete clonal evolution of P12 tumor.
Figure S10 from Dissecting the origin of heterogeneity in uterine and ovarian carcinosarcomas
Authors: Sertier Anne-Sophie , Ferrari Anthony , Pommier Roxane M. , Treilleux Isabelle , Boyault Sandrine , Devouassoux-Shisheboran Mojgan , Kielbassa Janice , Thomas Emilie , Tonon Laurie , Le Texier Vincent , Charreton Amandine , Morel Anne-Pierre , Floquet Anne , Joly Florence , Berton-Rigaud Dominique , Ferron Gwenaël , Arnould Laurent , Croce Sabrina , Bataillon Guillaume , Saintigny Pierre , Mery-Lamarche Eliane , Sagan Christine , Senaratne Aruni P. , Gut Ivo G. , Calvo Fabien , Viari Alain , Ouzounova Maria , Ray-Coquard Isabelle , Puisieux Alain
Published: April 2023

Analysis of common genomic events between paired tumor samples.
Data from Dissecting the Origin of Heterogeneity in Uterine and Ovarian Carcinosarcomas
Authors: Sertier Anne-Sophie , Ferrari Anthony , Pommier Roxane M. , Treilleux Isabelle , Boyault Sandrine , Devouassoux-Shisheboran Mojgan , Kielbassa Janice , Thomas Emilie , Tonon Laurie , Le Texier Vincent , Charreton Amandine , Morel Anne-Pierre , Floquet Anne , Joly Florence , Berton-Rigaud Dominique , Ferron Gwenaël , Arnould Laurent , Croce Sabrina , Bataillon Guillaume , Saintigny Pierre , Mery-Lamarche Eliane , Sagan Christine , Senaratne Aruni P. , Gut Ivo G. , Calvo Fabien , Viari Alain , Ouzounova Maria , Ray-Coquard Isabelle , Puisieux Alain
Published: April 2023

Gynecologic carcinosarcomas (CS) are biphasic neoplasms composed of carcinomatous (C) and sarcomatous (S) malignant components. Because of their rarity and histologic complexity, genetic and functional studies on CS are scarce and the mechanisms of initiation and development remain largely unknown.

Whole-genome analysis of the C and S components reveals shared genomic alterations, thus emphasizing the clonal evolution of CS. Reconstructions of the evolutionary history of each tumor further reveal that C and S samples are composed of both ancestral cell populations and component-specific subclones, supporting a common origin followed by distinct evolutionary trajectories. However, while we do not find any recurrent genomic features associated with phenotypic divergence, transcriptomic and methylome analyses identify a common mechanism across the cohort, the epithelial-to-mesenchymal transition (EMT), suggesting a role for nongenetic factors in inflicting changes to cellular fate. Altogether, these data accredit the hypothesis that CS tumors are driven by both clonal evolution and transcriptomic reprogramming, essential for susceptibility to transdifferentiation upon encountering environmental cues, thus linking CS heterogeneity to genetic, transcriptomic, and epigenetic influences.
Significance:
We have provided a detailed characterization of the genomic landscape of CS and identified EMT as a common mechanism associated with phenotypic divergence, linking CS heterogeneity to genetic, transcriptomic, and epigenetic influences.
Subungual Keratoacanthoma: Typical and Atypical Presentations of an Uncommon Nail Tumor
Authors: Figueroa-Ramos Grecia , Gatica-Torres Michelle , López-López Karla Elizabeth , Domínguez-Cherit Judith
Published: April 2023

Introduction: Keratoacanthoma (KA) is a group of tumors of epidermal origin with controversial nature. Subungual keratoacanthoma (SUKA) is a rare and destructive variant with more aggressive behavior. SUKA appears as a rapidly growing, painful tumor beneath the nail plate that rapidly progresses to a mass that can measure up to 2 cm. The toe location is unusual. The diagnosis must be made based on the correlation of clinical, radiological, and histopathological findings. Case Presentation: We present two cases of patients diagnosed with SUKAs with different clinical presentations which ranged from very typical to uncommon one. Both cases were treated with simple excision without recurrences. Conclusion: SUKA is a rare subungual tumor. Nail bed location represents a more difficult diagnostic challenge. SUKA should be suspected in the context of persistent and progressive pain on a finger or toe, once more frequent painful tumors have been ruled out.