bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of the Large Millimeter Telescope in Black Hole Science with the Next-Generation Event Horizon Telescope
Authors: Bustamante Sandra , Blackburn Lindy , Narayanan Gopal , Schloerb F. Peter , Hughes David
Published: December 2022

The landmark black hole images recently taken by the Event Horizon Telescope (EHT) have allowed the detailed study of the immediate surroundings of supermassive black holes (SMBHs) via direct imaging. These tantalizing early results motivate an expansion of the array, its instrumental capabilities, and dedicated long-term observations to resolve and track faint dynamical features in the black hole jet and accretion flow. The next-generation Event Horizon Telescope (ngEHT) is a project that plans to double the number of telescopes in the VLBI array and extend observations to dual-frequency 230 + 345 GHz, improving total and snapshot coverage, as well as observational agility. The Large Millimeter Telescope (LMT) is the largest sub-mm single dish telescope in the world at 50 m in diameter, and both its sensitivity and central location within the EHT array make it a key anchor station for the other telescopes. In this work, we detail current and planned future upgrades to the LMT that will directly impact its Very Large Baseline Interferometry (VLBI) performance for the EHT and ngEHT. These include the commissioning of a simultaneous 230 + 345 GHz dual-frequency, dual-polarization heterodyne receiver, improved real-time surface measurement and setting, and improvements to thermal stability, which should enable expanded daytime operation. We test and characterize the performance of an improved LMT joining future ngEHT observations through simulated observations of Sgr A* and M 87.
Applications of the Source-Frequency Phase-Referencing Technique for ngEHT Observations
Authors: Jiang Wu , Zhao Guang-Yao , Shen Zhi-Qiang , Rioja María J. , Dodson Richard , Cho Ilje , Zhao Shan-Shan , Eubanks Marshall , Lu Ru-Sen
Published: December 2022

The source-frequency phase-referencing (SFPR) technique has been demonstrated to have great advantages for mm-VLBI observations. By implementing simultaneous multi-frequency receiving systems on the next-generation Event Horizon Telescope (ngEHT) antennas, it is feasible to carry out a frequency phase transfer (FPT) which could calibrate the non-dispersive propagation errors and significantly increase the phase coherence in the visibility data. Such an increase offers an efficient approach for a weak source or structure detection. The SFPR also makes it possible for high-precision astrometry, including the core-shift measurements up to sub-mm wavelengths for Sgr A*, M 87*, etc. We also briefly discuss the technical and scheduling considerations for future SFPR observations with the ngEHT.
Representing the Silk Road: Literature and Images between China and Japan during the Cold War
Authors: Yin Zhixi
Published: December 2022

The essay focused on the TV documentary series The Silk Road and discussed the significance of this co-production between China and Japan. Two television stations, NHK and CCTV, provided each other with technical support to create a new image of the Silk Road in 1980. They attempted to rediscover the cultural relationship in Asia. Japanese Oriental studies were either the base for this co-production or the source of trouble. CCTV needed to utilize and overcome Japanese technology and the resources from Oriental studies, to represent national culture and identity through images. On the other hand, Japan once again sought ways to represent the Asian “others.” However, the challenge was making it relative to Orientalism and imperialism. This essay also compares the two versions and suggests that both ancient Asian cultural histories that they represented through images reflected the contemporaneous political situation of the Cold War. In CCTV’s version, the images of the Silk Road became a symbol of the re-establishment of China’s national identity, including the imagination of a “multi-ethnic state” and a “community of cultural memory that unites East and West”. Furthermore, this version also represented the trade with neighboring states. Each of these elements had a realistic role in the political environment of 1980. On the other hand, NHK’s version contains a narrative to prove Japan is the last stop on the Silk Road. Moreover, before this documentary, Japanese literature had long sought the “unknown” of the Silk Road and became a strong intellectual foundation for The Silk Road. The narrative of Japan “being a part of the Silk Road, but unlike in colonialism, as a traveler” is what Yasushi Inoue repeatedly expressed in his literary works and appeared to have been passed on through the images of the documentary. Carrying the negative legacy of Japanese imperialism, then being caught between the United States and the Socialist bloc, and having a difficult choice of political identity, Japanese intellectuals refrained from expressing their political positions and chose to describe cultural history from the “traveler’s” perspective. This essay suggests this is an attempt to redefine Japan on the cultural map of Asia and indirectly to break through the polarization of capitalism and socialism in the Cold War.
Factors Associated with Mental Health among Malaysian University Music Students: Roles of Fear of COVID-19, Nomophobia, Loneliness, Sleep Quality, and Socioeconomic Status
Authors: Zhuang Chunmei , Jenatabadi Hashem Salarzadeh
Published: December 2022

Previous mental health studies have shown higher levels of anxiety, stress, and depression symptoms among university music students. In general, some similar findings have been observed for Malaysian music university students. In diagnosing the complications of mental health, there is consensus that it is essential to develop and evaluate a model oriented toward mental health illness prevention and treatment. To date, a suitable pattern for estimating mental health in terms of anxiety, stress, and depression among music university students is lacking. To fill this gap, we collected the necessary data from 691 music and 871 general students who were students for one year. The introduced pattern includes socioeconomic status, fear of COVID-19, nomophobia, sleep quality, loneliness, and mental health. Our data analysis proved that the levels of anxiety, depression, and stress of music students were lower than those of general students. Unlike some previous studies, in this study, the fear of COVID-19 and nomophobia didn’t have the most significant impact on mental health. The most significant impacts were related to sleep quality and loneliness. These findings have the potential to inform health promotion and services in the music education system.
Future Time Perspective, Priority of Social Goals, and Friend Networks in Old Age: Evidence for Socioemotional Selectivity Theory Using Subjective Age Gap
Authors: Bae Moonjo , Jeon Sesong , Walker Katie
Published: December 2022

Several studies have focused on population aging, with a focus on the relationship between age and the main concepts of the socioemotional selectivity theory, but many do not report consistent results. Therefore, this study sought to better understand how the socioemotional selective theory informs our understanding of the elderly in Korea. More specifically, it aimed at observing how age groups differ in regards to future time perspective, social goals, and friend networks. Data were collected from 271 elderly people (M = 72.98 years old, SD = 5.63) using questionnaires. The statistical program SPSS 25.0 was used to perform descriptive statistical analyses, reliability analyses, and ANOVAs. The findings indicated that the Korean elderly participants perceived their subjective age to be younger than their chronological age. Furthermore, if they perceived their subjective age to be older than their chronological age, they were more likely to report that their network of friends was smaller than they desired. Lastly, depending on their age, the Korean elderly participants reported different priorities of the goals they wished to pursue. These results could help researchers, clinical practitioners, and policymakers to better understand the unique differences in the Korean elderly.
Development of a Physical Activity Triggers Questionnaire
Authors: Wang Yunbo , Kang Hyoung-Kil
Published: December 2022

Background: This study aimed to develop a Physical Activity Triggers Questionnaire for Chinese college students and to evaluate its reliability and validity. Methods: On the theoretical basis of the Fogg behavior model and semi-open interviews, an initial questionnaire with 18 items was compiled. The initial questionnaire was administered to 575 students, and to examine its reliability and validity, item discrimination analysis, correlation analysis, homogeneity test, and exploratory factor analysis were conducted using SPSS 26.0. After the examination of the initial questionnaire, the initial 18 items were reduced to 14. The 14-item questionnaire was administered to 621 college students, and with the data, correlation analysis, confirmatory factor analysis, validity test, and reliability test were conducted. Results: To examine the psychometric properties of the 18 items, exploratory factor analysis and confirmatory factor analysis were conducted, and their reliability and validity were examined. After the first round of item development analysis, four items were removed, and a triggers questionnaire with 14 items was developed. The 14 items had three dimensions, including spark, signal, and facilitator triggers, and the cumulative explained variance of the three dimensions was 61.21%. The confirmatory factor analysis of the three dimensions of the 14 items indicated appropriate scale fit indices. The internal consistency reliability, split-half reliability, and test–retest reliability of the 14 items were 0.925, 0.821, and 0.860, respectively, showing that the items have appropriate reliability. Conclusions: The Physical Activity Triggers Questionnaire of the study has acceptable reliability and validity. It is the first questionnaire to measure Chinese college students’ triggers of physical activity and will provide a new basis for the understanding of psychometric properties of physical activity triggers. In addition, the future findings collected from the developed triggers questionnaire can be used to develop strategies to promote health among college students.
Pace by Nino Migliori: Examination, Analysis and Treatment of a Contemporary Color Photography Experimental Artwork
Authors: D’Amico Maria Cristina , Gianferrari Melissa , Del Bianco Andrea
Published: December 2022

The present work intends to focus on the examination, analysis and treatment of Pace, a contemporary color photography experimental artwork created by Nino Migliori in 1973. Given the particular nature of the artifact, in which the photographic materials and the media of contemporary art coexist in a complex interaction, an in-depth study of its creation, structure and components was deemed necessary to plan and perform an adequate conservation treatment. The preliminary research was conducted in order to contextualize the artwork within the author’s production, to understand the creation process, to identify the photographic technique of the print and the stratigraphic sequencing, and to characterize the constituent materials of each layer through non-invasive and micro-invasive diagnostic analysis. The severe detachments and lifts of the print from the underlying aluminum panel and the subsequent planarity alterations of the photograph, strongly compromised the legibility of the artwork and put its future conservation at risk. Therefore, the elaboration of a minimally invasive intervention methodology was required. Approaching contemporary works of art, which are at the same time also photographic objects, can present some theoretical and practical challenges, and in the near future lots of professionals in the field of art conservation are probably going to face analogue conservation problems on similar objects. The results obtained through the preliminary study phase and the conservation treatment may offer a good starting point for further research on how to examine, analyze and treat this kind of photographic objects.
MaHAK5, a Potassium Transporter of Banana, Enhanced Potassium Uptake in Transgenic Arabidopsis under Low Potassium Conditions
Authors: Wu Bangting , Xie Yanling , Xiang Dandan , Yi Ganjun , Liu Hong , Li Chunyu , Liu Siwen
Published: December 2022

Potassium (K+) is one of the most important macronutrients for plant growth and development. It is generally accepted that the KUP/HAK/KT transporters play essential roles in K+ uptake at low concentrations. However, their physiological functions in bananas remain unknown. Here, we cloned MaHAK5 and analyzed its functions in banana (Musa acuminata). Gene expression analysis showed that MaHAK5 was upregulated in the roots and leaves in the early stage of low K+ (LK) stress. MaHAK5 was localized in the cytomembrane. The expression of MaHAK5 improved the growth of the low K+-sensitive yeast mutant R5421 at different K+ supply levels. Overexpression of MaHAK5 in Arabidopsis thaliana significantly enhanced the ability for K+ uptake and increased the chlorophyll content under LK stress. These results indicate that MaHAK5 plays a crucial role in maintaining K+ uptake in bananas.
An Inverse Agonist GSK5182 Increases Protein Stability of the Orphan Nuclear Receptor ERRγ via Inhibition of Ubiquitination
Authors: Na Soon-Young , Kim Ki-Sun , Jung Yoon Seok , Kim Don-Kyu , Kim Jina , Cho Sung Jin , Lee In-Kyu , Chung Jongkyeong , Kim Jeong-Sun , Choi Hueng-Sik
Published: December 2022

The orphan nuclear receptor, estrogen-related receptor γ (ERRγ) is a constitutively active transcription factor involved in mitochondrial metabolism and energy homeostasis. GSK5182, a specific inverse agonist of ERRγ that inhibits transcriptional activity, induces a conformational change in ERRγ, resulting in a loss of coactivator binding. However, the molecular mechanism underlying the stabilization of the ERRγ protein by its inverse agonist remains largely unknown. In this study, we found that GSK5182 inhibited ubiquitination of ERRγ, thereby stabilizing the ERRγ protein, using cell-based assays and confocal image analysis. Y326 of ERRγ was essential for stabilization by GSK5182, as ligand-induced stabilization of ERRγ was not observed with the ERRγ-Y326A mutant. GSK5182 suppressed ubiquitination of ERRγ by the E3 ligase Parkin and subsequent degradation. The inhibitory activity of GSK5182 was strong even when the ERRγ protein level was elevated, as ERRγ bound to GSK5182 recruited a corepressor, small heterodimer partner-interacting leucine zipper (SMILE), through the activation function 2 (AF-2) domain, without alteration of the nuclear localization or DNA-binding ability of ERRγ. In addition, the AF-2 domain of ERRγ was critical for the regulation of protein stability. Mutants in the AF-2 domain were present at higher levels than the wild type in the absence of GSK5182. Furthermore, the ERRγ-L449A/L451A mutant was no longer susceptible to GSK5182. Thus, the AF-2 domain of ERRγ is responsible for the regulation of transcriptional activity and protein stability by GSK5182. These findings suggest that GSK5182 regulates ERRγ by a unique molecular mechanism, increasing the inactive form of ERRγ via inhibition of ubiquitination.
Sodium Alginate-g-acrylamide/acrylic Acid Hydrogels Obtained by Electron Beam Irradiation for Soil Conditioning
Authors: Manaila Elena , Craciun Gabriela , Calina Ion Cosmin
Published: December 2022

Being both a cause and a victim of water scarcity and nutrient deficiency, agriculture as a sustainable livelihood is dependent now on finding new suport solutions. Biodegradable hydrogels usage as soil conditioners may be one of the most effective solutions for irrigation efficiency improvement, reducing the quantity of soluble fertilizers per crop cycle and combating pathogens, due to their versatility assured by both obtaining method and properties. The first goal of the work was the obtaining by electron beam irradiation and characterization of some Sodium Alginate-g-acrylamide/acrylic Acid hydrogels, the second one being the investigation of their potential use as a soil conditioner by successive experiments of absorption and release of two different aqueous nutrient solutions. Alginate-g-acrylamide/acrylic Acid hydrogels were obtained by electron beam irradiation using the linear accelerator ALID 7 at 5.5 MeV at the irradiation doses of 5 and 6 kGy. For this were prepared monomeric solutions that contained 1 and 2% sodium alginate, acrylamide and acrylic acid in ratios of 1:1 and 1.5:1, respectively, for the obtaining of materials with hybrid properties derived from natural and synthetic components. Physical, chemical, structural and morphological characteristics of the obtained hydrogels were investigated by specific analysis using swelling, diffusion and network studies and Fourier Transform Infrared Spectroscopy and Scanning Electron Microscopy. Four successive absorption and release experiments of some synthetic and natural aqueous solutions with nutrients were performed.
A Genome-Wide Association Study of 2304 Extreme Longevity Cases Identifies Novel Longevity Variants
Authors: Bae Harold , Gurinovich Anastasia , Karagiannis Tanya T. , Song Zeyuan , Leshchyk Anastasia , Li Mengze , Andersen Stacy L. , Arbeev Konstantin , Yashin Anatoliy , Zmuda Joseph , An Ping , Feitosa Mary , Giuliani Cristina , Franceschi Claudio , Garagnani Paolo , Mengel-From Jonas , Atzmon Gil , Barzilai Nir , Puca Annibale , Schork Nicholas J. , Perls Thomas T. , Sebastiani Paola
Published: December 2022

We performed a genome-wide association study (GWAS) of human extreme longevity (EL), defined as surviving past the 99th survival percentile, by aggregating data from four centenarian studies. The combined data included 2304 EL cases and 5879 controls. The analysis identified a locus in CDKN2B-AS1 (rs6475609, p = 7.13 × 10−8) that almost reached genome-wide significance and four additional loci that were suggestively significant. Among these, a novel rare variant (rs145265196) on chromosome 11 had much higher longevity allele frequencies in cases of Ashkenazi Jewish and Southern Italian ancestry compared to cases of other European ancestries. We also correlated EL-associated SNPs with serum proteins to link our findings to potential biological mechanisms that may be related to EL and are under genetic regulation. The findings from the proteomic analyses suggested that longevity-promoting alleles of significant genetic variants either provided EL cases with more youthful molecular profiles compared to controls or provided some form of protection from other illnesses, such as Alzheimer’s disease, and disease progressions.
A Comprehensive Biomarker Analysis of Microsatellite Unstable/Mismatch Repair Deficient Colorectal Cancer Cohort Treated with Immunotherapy
Authors: Élez Elena , Mulet-Margalef Núria , Sanso Miriam , Ruiz-Pace Fiorella , Mancuso Francesco M. , Comas Raquel , Ros Javier , Argilés Guillem , Martini Giulia , Sanz-Garcia Enrique , Baraibar Iosune , Salvà Francesc , Noguerido Alba , Cuadra-Urteaga Jose Luis , Fasani Roberta , Garcia Ariadna , Jimenez Jose , Aguilar Susana , Landolfi Stefania , Hernández-Losa Javier , Braña Irene , Nuciforo Paolo , Dienstmann Rodrigo , Tabernero Josep , Salazar Ramon , Vivancos Ana
Published: December 2022

The search for immunotherapy biomarkers in Microsatellite Instability High/Deficient Mismatch Repair system (MSI-H/dMMR) metastatic colorectal cancer (mCRC) is an unmet need. Sixteen patients with mCRC and MSI-H/dMMR (determined by either immunohistochemistry or polymerase chain reaction) treated with PD-1/PD-L1 inhibitors at our institution were included. According to whether the progression-free survival with PD-1/PD-L1 inhibitors was longer than 6 months or shorter, patients were clustered into the IT-responder group (n: 9 patients) or IT-resistant group (n: 7 patients), respectively. In order to evaluate determinants of benefit with PD-1/PD-L1 inhibitors, we performed multimodal analysis including genomics (through NGS panel tumour-only with 431 genes) and the immune microenvironment (using CD3, CD8, FOXP3 and PD-L1 antibodies). The following mutations were more frequent in IT-resistant compared with IT-responder groups: B2M (4/7 versus 2/9), CTNNB1 (2/7 versus 0/9), and biallelic PTEN (3/7 versus 1/9). Biallelic ARID1A mutations were found exclusively in the IT-responder group (4/9 patients). Tumour mutational burden did not correlate with immunotherapy benefit, neither the rate of indels in homopolymeric regions. Of note, biallelic ARID1A mutated tumours had the highest immune infiltration and PD-L1 scores, contrary to tumours with CTNNB1 mutation. Immune microenvironment analysis showed higher densities of different T cell subpopulations and PD-L1 expression in IT-responders. Misdiagnosis of MSI-H/dMMR inferred by discordances between immunohistochemistry and polymerase chain reaction was only found in the IT-resistant population (3/7 patients). Biallelic ARID1A mutations and Wnt signalling activation through CTNNB1 mutation were associated with high and low T cell immune infiltrates, respectively, and deserve special attention as determinants of response to PD-1/PD-L1 inhibitors. The non-MSI-H phenotype in dMMR is associated with poor benefit to immunotherapy. Our results suggest that mechanisms of resistance to immunotherapy are multi-factorial.
Quality Control of Human Pluripotent Stem Cell Colonies by Computational Image Analysis Using Convolutional Neural Networks
Authors: Mamaeva Anastasiya , Krasnova Olga , Khvorova Irina , Kozlov Konstantin , Gursky Vitaly , Samsonova Maria , Tikhonova Olga , Neganova Irina
Published: December 2022

Human pluripotent stem cells are promising for a wide range of research and therapeutic purposes. Their maintenance in culture requires the deep control of their pluripotent and clonal status. A non-invasive method for such control involves day-to-day observation of the morphological changes, along with imaging colonies, with the subsequent automatic assessment of colony phenotype using image analysis by machine learning methods. We developed a classifier using a convolutional neural network and applied it to discriminate between images of human embryonic stem cell (hESC) colonies with “good” and “bad” morphological phenotypes associated with a high and low potential for pluripotency and clonality maintenance, respectively. The training dataset included the phase-contrast images of hESC line H9, in which the morphological phenotype of each colony was assessed through visual analysis. The classifier showed a high level of accuracy (89%) in phenotype prediction. By training the classifier on cropped images of various sizes, we showed that the spatial scale of ~144 μm was the most informative in terms of classification quality, which was an intermediate size between the characteristic diameters of a single cell (~15 μm) and the entire colony (~540 μm). We additionally performed a proteomic analysis of several H9 cell samples used in the computational analysis and showed that cells of different phenotypes differentiated at the molecular level. Our results indicated that the proposed approach could be used as an effective method of non-invasive automated analysis to identify undesirable developmental anomalies during the propagation of pluripotent stem cells.
The Greatwall–Endosulfine Switch Accelerates Autophagic Flux during the Cell Divisions Leading to G1 Arrest and Entry into Quiescence in Fission Yeast
Authors: Vázquez-Bolado Alicia , López-San Segundo Rafael , García-Blanco Natalia , Rozalén Ana Elisa , González-Álvarez Daniel , Suárez M. Belén , Pérez-Hidalgo Livia , Moreno Sergio
Published: December 2022

Entry into quiescence in the fission yeast Schizosaccharomyces pombe is induced by nitrogen starvation. In the absence of nitrogen, proliferating fission yeast cells divide twice without cell growth and undergo cell cycle arrest in G1 before becoming G0 quiescent cells. Under these conditions, autophagy is induced to produce enough nitrogen for the two successive cell divisions that take place before the G1 arrest. In parallel to the induction of autophagy, the Greatwall–Endosulfine switch is activated upon nitrogen starvation to down-regulate protein phosphatase PP2A/B55 activity, which is essential for cell cycle arrest in G1 and implementation of the quiescent program. Here we show that, although inactivation of PP2A/B55 by the Greatwall–Endosulfine switch is not required to promote autophagy initiation, it increases autophagic flux at least in part by upregulating the expression of a number of autophagy-related genes.
Age- and Stage-Dependent Prostate Cancer Aggressiveness Associated with Differential Notch Signaling
Authors: Orzechowska Magdalena Julita , Anusewicz Dorota , Bednarek Andrzej K.
Published: December 2022

Prostate cancer (PC) remains a worldwide challenge, as does the question of how to distinguish its indolent from its aggressive form to reconcile proper management of the disease with age-related life expectations. This study aimed to differentiate the Notch-driven course of PC regarding patients’ ages and stage of their disease. We analyzed 397 PC samples split into age subgroups of ≦55, 60–70, and >70 years old, as well as early vs. late stage. The clinical association of Notch signaling was evaluated by DFS and UpSet analyses. The clustering of downstream effectors was performed with ExpressCluster. Finally, for the most relevant findings, functional networks were constructed with MCODE and stringApp. The results have been validated with an independent cohort. We identified specific patterns of Notch expression associated with unfavorable outcomes, which were reflected by entering into a hybrid epithelial/mesenchymal state and thus reaching tumor plasticity with its all consequences. We characterized the molecular determinants of the age-related clinical behavior of prostate tumors that stem from different invasive properties depending on the route of the EMT program. Of the utmost relevance is the discovery of age- and stage-specific combinations of the Notch molecules predicting unfavorable outcomes and constituting a new prognostic and therapeutic approach for PCs.
Hand-Foot-and-Mouth Disease-Associated Enterovirus and the Development of Multivalent HFMD Vaccines
Authors: Zhang Xinglong , Zhang Yifan , Li Heng , Liu Longding
Published: December 2022

Hand-foot-and-mouth disease (HFMD) is an infectious disease of children caused by more than 20 types of enteroviruses, with most cases recovering spontaneously within approximately one week. Severe HFMD in individual children develops rapidly, leading to death, and is associated with other complications such as viral myocarditis and type I diabetes mellitus. The approval and marketing of three inactivated EV-A71 vaccines in China in 2016 have provided a powerful tool to curb the HFMD epidemic but are limited in cross-protecting against other HFMD-associated enteroviruses. This review focuses on the epidemiological analysis of HFMD-associated enteroviruses since the inactivated EV-A71 vaccine has been marketed, collates the progress in the development of multivalent enteroviruses vaccines in different technical routes reported in recent studies, and discusses issues that need to be investigated for safe and effective HFMD multivalent vaccines.
Unexpected Poor Vision within 24 h of Uneventful Phacoemulsification Surgery—A Review
Authors: Rosen Harry , Vernon Stephen A.
Published: December 2022

Review on day one post uneventful phacoemulsification surgery is no longer standard practice due to the infrequency of complications when using modern cataract removal techniques. Clinicians are therefore likely to be unfamiliar with the potential causes of reduced vision when presented with a patient in the immediate postoperative period. The purpose of this review is to discuss the various differential causes of early visual loss, for the benefit of clinicians presented with similar patients in emergency care, with the use of an illustrative clinical case of paracentral acute middle maculopathy (PAMM), which recently presented to the authors. A thorough literature search on Google Scholar was conducted, and only causes of visual loss that would manifest within 24 h postoperatively were included. Complications are inherently rare in this period; however, various optical, anterior segment, lens-related and posterior segment causes have been identified and discussed. Front-line clinicians should be aware of these differentials with different mechanisms. PAMM remains to be the only cause of unexpected visual loss within this time frame that may have no abnormal findings on clinical examination.
Pre- and Post-Operative Hamstring Autograft ACL Reconstruction Isokinetic Knee Strength Assessments of Recreational Athletes
Authors: Güzel Nizamettin , Yılmaz Ali Kerim , Genç Ahmet Serhat , Karaduman Emre , Kehribar Lokman
Published: December 2022

Background and Objectives: Anterior cruciate ligament (ACL) injuries are common injuries with a high incidence among people with high physical activity levels. Therefore, ACL reconstruction (ACLR) is one of the most common surgical procedures performed in sports medicine. This study aims to compare the pre- and 6-month post-operative isokinetic knee strengths in healthy (HK) and ACL knees of patients who underwent semitendinous/gracilis (ST/G) ACLR. Materials and Methods: A retrospective cohort of 21 recreational athletes who underwent ST/G ACLR by the same surgeon were evaluated. The pre- and 6-month post-operative isokinetic knee extension (Ex) and flexion (Flx) strengths of the HK and ACLR patients were evaluated in a series consisting of three different angular velocities (60, 180 and 240°/s). Of all the findings, peak torque (PT) and hamstring/quadriceps (H/Q) parameters were evaluated. Results: There was a significant improvement in post-operative Lysholm, Tegner and IKDC scores compared to pre-operative scores (p < 0.05). There were significant differences in pre-operative and post-operative knee Ex and Flx strengths at angular velocities of 60°, 180° and 240°/s in both the ACLR and HK groups (p < 0.001). There was no significance at 240°/s Flx for ACLR (p > 0.05). As for H/Q ratios, there was a significant difference between pre- and post-operative values only at 60°/s angular velocity in both ACLR and HC (p < 0.005). Conclusions: The pre-operative and 6-month post-operative results of the ST/G ACLR showed that there was a high level of recovery, particularly in quadriceps strength, while the increase in strength was less in the hamstring. The significance observed at 60°/s in H/Q ratios was within normal ranges. It can be argued that the ST/G ACLR method is feasible for people with high physical activity levels and for athletes.
Non-Hormonal Treatment Options for Regulation of Menstrual Cycle in Adolescents with PCOS
Authors: Reiser Elisabeth , Lanbach Julia , Böttcher Bettina , Toth Bettina
Published: December 2022

Menstrual irregularities are one of the main clinical symptoms caused by polycystic ovary syndrome (PCOS). Pharmacological treatment options for non-fertility indications to restore menstrual frequency play an important role in the management of PCOS. Oral contraceptive pills are commonly prescribed for adolescents with menstrual irregularities, however, when contraindicated or poorly tolerated, further pharmacological therapy is required. This systematic literature research aims to provide an overview concerning the effects of non-hormonal pharmacological treatment options on menstrual irregularities in adolescents suffering from PCOS. A systematic literature search in PubMed, Cochrane, Embase, Bio-SISS and Web of Science was performed, including literature from January 1998 to September 2022, using specific keywords in order to find related studies. n = 265 studies were identified of which n = 164 were eligible for further evaluation. Only four placebo-controlled studies were identified, with diverging inclusion and exclusion criteria. Available data on specific non-hormonal off-label use medication primarily consisted of metformin, Glucagon-like peptide 1 receptor agonists, thiazolidinediones, anti-androgen agents (spironolactone, finasteride, flutamide) and supplements (chromium picolinate, myo-inositol). However, only a few have partly pointed out beneficial effects on improving menstrual frequency in patients diagnosed with PCOS. In summary, metformin in dosages of 1500–2550 g/day, GLP-1—analogues and supplements were effective in regulation of menstrual cycles in adolescents diagnosed with PCOS. Menstrual frequency in adolescents with PCOS is essential to prevent hypoestrogenism with long-term consequences. In this context, MET is the most effective and cost- efficient in overweight adolescent girls, also showing beneficial effects in the regulation of insulin sensitivity, especially if COCs are contraindicated or not well-tolerated. Further studies are needed to evaluate therapies in lean and normal-weight girls with PCOS.
Renal Tissue Expression of BAFF and BAFF Receptors Is Associated with Proliferative Lupus Nephritis
Authors: Marín-Rosales Miguel , Palafox-Sánchez Claudia Azucena , Franco-Topete Ramón Antonio , Carrillo-Ballesteros Francisco Josué , Cruz Alvaro , Salazar-Camarena Diana Celeste , Muñoz-Valle José Francisco , Ramos-Solano Francisco
Published: December 2022

Background: The B-cell activating factor (BAFF) controls the maturation and survival of B cells. An imbalance in this cytokine has been associated with systemic autoimmunity in SLE and lupus nephritis (LN). However, few investigations have evaluated the tissular expression of BAFF in LN. This study aimed to associate BAFF system expression at the tissular level with the proliferative LN classes. Methods: The analysis included eighteen kidney tissues, with sixteen LN (class III = 5, class IV = 6, class III/IV+V = 4, and class V = 1), and two controls. The tissular expression was evaluated with an immunochemistry assay. A Cytation5 imaging reader and ImageJ software were used to analyze the quantitative expression. A p-value < 0.05 was considered significant. Results: The expressions of BAFF, A proliferation-inducing ligand (APRIL), and their receptors were observed in glomerular, tubular, and interstitial zones, with BAFF being the most strongly expressed in the overall analysis. BAFF-Receptor (BR3), transmembrane activator and CALM interactor (TACI), and B-Cell maturation antigen (BCMA) displayed higher expressions in LN class IV in all zones analyzed (p < 0.05). Additionally, a positive correlation was found between APRIL, TACI, and BCMA at the glomerular level; BCMA and APRIL in the interstitial zone; and BR3, TACI, and BCMA in the tubule (p < 0.05). Conclusions: The expression of BAFF and BAFF receptors is mainly associated with LN class IV, emphasizing the participation of these receptors as an essential pathogenic factor in kidney involvement in SLE patients.
Autonomic Nerve Involvement in Post-Acute Sequelae of SARS-CoV-2 Syndrome (PASC)
Authors: Chung Tae Hwan , Azar Antoine
Published: December 2022

The novel SARS-CoV-2 virus and resulting COVID-19 global pandemic emerged in 2019 and continues into 2022. While mortality from COVID-19 is slowly declining, a subset of patients have developed chronic, debilitating symptoms following complete recovery from acute infection with COVID-19. Termed as post-acute sequelae of SARS-CoV-2 syndrome (PASC), the underlying pathophysiology of PASC is still not well understood. Given the similarity between the clinical phenotypes of PASC and postural orthostatic tachycardia syndrome (POTS), it has been postulated that dysautonomia may play a role in the pathophysiology of PASC. However, there have been only a few studies that have examined autonomic function in PASC. In this retrospective study, we performed an analysis of autonomic nerve function testing in PASC patients and compared the results with those of POTS patients and healthy controls. Our results suggest that a significant number of PASC patients have abnormal autonomic function tests, and their clinical features are indistinguishable from POTS.
A Year of Bio-Monitoring (2021): Presence of Algae of the Genus Alexandrium, Dinophysis, Prorocentrum and Non-Compliance for Paralytic Toxins and Lipophilic Toxins in Bivalve Mollusks Bred in Sardinia (W Mediterranean Sea)
Authors: Lorenzoni Giuseppa , Bazzoni Anna Maria , Cangini Monica , Dall’Ara Sonia , Melillo Rita , Mudadu Alessandro Graziano , Cau Simona , Soro Barbara , Ledda Salvatore , Piras Gabriella , Tedde Tiziana , Salza Sara , Arras Igor , Porqueddu Giuseppa , Meloni Domenico
Published: December 2022

Bivalve mollusk production represents the principal aquaculture activity in Sardinia (40°03′ N, 9°05′ E). In 2021, 859 water samples and 1270 mollusk samples were analyzed. The species Alexandrium minutum caused the accumulation of Paralytic Shellfish Toxins (PST) in three samples of bivalve mollusks. Dinophysis acuminata complex caused the accumulation of lipophilic toxins (LTs) belonging to the okadaic acid group (OAs) in 18 samples of bivalve mollusks. The research of paralytic shellfish toxins (PSTs) in shellfish samples has been carried out with LC-FLD, as mentioned in the AOAC 2005 Official Method 2005.06. The determination of LTs was carried out by LC-MS/MS analysis. DTX2, belonging to the group of OA toxins, was detected for the first time in Sardinia, in mussels sampled in Tortolì. The presence of Dinophysis and Prorocentrum species was correlated with the accumulation of the OA toxin group in bivalve mollusks, showing a certain repeatability at certain times of the year in the areas included in the study. The results of the present study can help to plan and organize more effective bio-monitoring sampling strategies.
Concept Design of a Hybrid Offshore Patrol Vessel
Authors: Ljulj Andrija , Slapničar Vedran , Grubišić Izvor , Mihanović Luka
Published: December 2022

This paper presents the proposal for the concept design of hybrid Offshore Patrol Vessels (OPV) that are suitable for countries with budget constraints. Many countries cannot afford two different classes of ships to answer the current civil and military challenges. Therefore, the proposed approach might be the most acceptable solution for these countries. The concept design covers hybrid OPVs that could include traditional navy capabilities such as anti-ship and anti-air warfare in addition to offshore patrol tasks. This enables a hybrid concept design model that incorporates major tasks of both patrol and corvette classes of ships. The solutions are achieved by applying a multi-attribute concept design procedure that was developed earlier for a generic naval vessel design. The procedure is customized to suit this purpose by applying appropriate design variables, parameter space constraints, updated ship propulsion systems, weapons, other equipment, and design requirements i.e., required areas, volumes, and electricity. The results are the Pareto optimal set of solutions that are used as initial designs in the subsequent phases of the design of the hybrid OPV. The earlier developed software named “MonakoPRB” is updated to suit the above-mentioned changes in the design procedure to generate hybrid OPV concept design solutions.
Experimental Thermal–Hydraulic Testing of a Mock-Up of the Fuel-Breeder Pin Concept for the EU-DEMO HCPB Breeding Blanket
Authors: Abou-Sena Ali , Ghidersa Bradut-Eugen , Zhou Guangming , Rey Joerg , Hernández Francisco A. , Lux Martin , Schlindwein Georg
Published: December 2022

The fusion program in the Karlsruhe Institute of Technology (KIT) leads the R&D of the DEMO helium-cooled pebble bed (HCPB) breeding blanket within the work package breeding blanket (WPBB) of the Eurofusion Consortium in the European Union (EU). A new design of the HCPB breeder zone, with a layout inspired by a nuclear reactor fuel rod arrangement, was developed recently and called the fuel-breeder pin concept. In addition, a mock-up (MU) of this fuel-breeder pin was designed and manufactured at KIT in order to test and validate its thermal–hydraulic performance. This paper reports on the results of the first experimental campaign dedicated to the fuel-breeder pin MU testing that was performed in the Helium Loop Karlsruhe (HELOKA) facility. The paper presents: (i) the integration of the fuel-breeder pin MU into the HELOKA loop including considerations of the experimental set-up, (ii) an overview of the plan for the experimental campaigns, and (iii) a discussion of the experimental results with a focus on aspects relevant for the validation of the thermal–hydraulic design of the HCPB breeder zone.
The Transcriptional Responses of Ectomycorrhizal Fungus, Cenococcum geophilum, to Drought Stress
Authors: Li Mingtao , Yuan Chao , Zhang Xiaohui , Pang Wenbo , Zhang Panpan , Xie Rongzhang , Lian Chunlan , Zhang Taoxiang
Published: December 2022

With global warming, drought has become one of the major environmental pressures that threaten the development of global agricultural and forestry production. Cenococcum geophilum (C. geophilum) is one of the most common ectomycorrhizal fungi in nature, which can form mycorrhiza with a large variety of host trees of more than 200 tree species from 40 genera of both angiosperms and gymnosperms. In this study, six C. geophilum strains with different drought tolerance were selected to analyze their molecular responses to drought stress with treatment of 10% polyethylene glycol. Our results showed that drought-sensitive strains absorbed Na and K ions to regulate osmotic pressure and up-regulated peroxisome pathway genes to promote the activity of antioxidant enzymes to alleviate drought stress. However, drought-tolerant strains responded to drought stress by up-regulating the functional genes involved in the ubiquinone and other terpenoid-quinone biosynthesis and sphingolipid metabolism pathways. The results provided a foundation for studying the mechanism of C. geophilum response to drought stress.
Antenna Delay-Independent Simultaneous Ranging for UWB-Based RTLSs
Authors: Shah Shashi , Chaudhary Sushank , Ullah Rizwan , Parnianifard Amir , Siddiqi Muhammad Zain , Vanichchanunt Pisit , Santipach Wiroonsak , Wuttisittikulkij Lunchakorn
Published: December 2022

The ultra-wideband (UWB)-based real-time localization system (RTLS) is a promising technology for locating and tracking assets and personnel in real-time within a defined indoor environment since it provides high-ranging accuracy. However, its performance can be affected by the underlying antenna delays of UWB nodes, which act as a source of error during range estimations. Usually, measurement of the antenna delays is performed separately as a dedicated standalone procedure. Such an additional measurement procedure makes the UWB-based RTLS more tedious with manual interventions. Moreover, the air-time occupancy during the transmission and reception of signaling messages for range estimations between UWB node pairs also limits the serviceable capability of these networks. In this regard, we present a novel simultaneous ranging scheme that requires limited air-time occupancy during range estimations between UWB node pairs and also compensates for the error from the antenna delays. This paper provides a detailed mathematical modeling, system design, and implementation procedure of the proposed scheme. The effectiveness of the proposed scheme for locating a mobile node in an indoor environment is validated through experimental analysis. The results show that, compared to the state-of-the-art two-way ranging (TWR) method, the proposed scheme eliminates the requirement of dedicated standalone antenna delay measurement procedures of the nodes, increases air efficiency through the provision of simultaneous ranging, and provides relative root-mean-square errors (RMSEs) improvement for range and position estimations of approximately 54.52% and 39.96%, respectively.
Residents’ Selection Behavior of Compensation Schemes for Construction Land Reduction: Empirical Evidence from Questionnaires in Shanghai, China
Authors: Lu Jianglin , Wang Keqiang , Liu Hongmei
Published: December 2022

Construction land reduction (CLR) was implemented in China to improve the efficiency of construction land use. CLR also limited the development of net reduction areas of CLR. By analyzing the Task-Quota-Financial-Benefit flow of CLR, this paper proposes three typical compensation schemes and uses the multivariate probit model to study residents’ selection behavior for these schemes. It is found that (1) in order to compensate for the losses caused by CLR to the reduced direct subjects, there can be three types of possible schemes: direct economic compensation (Scheme I), in situ (Scheme II) and off-site (Scheme III) enhancement of development capacity. (2) The more reasonable the compensation standard, the greater the employment pressure in the township and the greater the township’s location disadvantage, which is why more residents prefer Scheme III. (3) The higher their family income and their family support pressure, the more they prefer Scheme III. (4) At this stage, there is no significant difference in the choice of compensation schemes between cadres and non-cadres. (5) The net planning reduction area prefers Scheme I, while other areas prefer Scheme II. The conclusions may provide insight into the demand for more reasonable compensation policies to ensure the sustainability of CLR.
Application of Solution Calorimetry to Determining the Fusion Enthalpy of an Arylaliphatic Compound at 298.15 K: n-Octadecanophenone
Authors: Yagofarov Mikhail I. , Balakhontsev Ilya S. , Sokolov Andrey A. , Solomonov Boris N.
Published: December 2022

Evaluating the temperature dependence of the fusion enthalpy is no trivial task, as any compound melts at a unique temperature. At the same time, knowledge of the fusion enthalpies under some common conditions, particularly at the reference temperature of 298.15 K, would substantially facilitate the comparative analysis and development of the predictive schemes. In this work, we continue our investigations of the temperature dependence of the fusion enthalpy of organic non-electrolytes using solution calorimetry. As an object of study, n-octadecanophenone, an arylaliphatic compound was chosen. The solvent appropriate for evaluating the fusion enthalpy at 298.15 K from the solution enthalpy of crystal was selected: p-xylene. The heat capacity and fusion enthalpy at the melting temperature were measured by differential scanning calorimetry to derive the fusion enthalpy at 298.15 K from the Kirchhoff’s law of Thermochemistry. An agreement between the independently determined values was demonstrated. This particular result opens a perspective for further studies of the fusion thermochemistry of arylaliphatic compounds at 298.15 K by solution calorimetry.
Identifying Parametric Models Used to Estimate Track Irregularities of a High-Speed Railway
Authors: Choi Sunghoon
Published: December 2022

This study aims to identify parametric models to estimate track irregularities in high-speed railways with simple acceleration measurements. The primary contribution of current research is the development of effective parametric models with smaller parameters. These parameters are derived from the measured data via a specialized track geometry inspection system. An adaptive Kalman filter algorithm, using the displacement estimated from the acceleration signals as the input and measured track irregularities as the output, is applied to obtain the model’s unknown parameters. These models are applied to acceleration measured from high-speed rail vehicles in operation, and track irregularities are estimated in spatial and wavelength domains. The estimated irregularities are compared to the track geometry inspection system’s results.