bluuebunny/crossref_metadata_2025 huggingface dataset:
Expanding the clinical role of the district nurse: legal aspects and practical experience
Authors: Kryakova M. Yu. , Drapkina O. M. , Shepel R. N. , Karakulina E. V. , Kupeeva I. A. , Shchegolev P. E. , Son I. M.
Published: December 2024

Aim. To justify a change in activities of a district nurse and the expansion of her clinical role.

Material and methods. An analysis  of  regulatory  legal  acts  governing  the  organization  of  primary  health care and the activities of a district nurse was conducted. A medical and sociological study was conducted — an anonymous questionnaire of district general practitioners, district nurses, patients and observations of the work of district general practitioners and district nurses in parallel in order to assess the activities of a district nurse in the current situation. Based on analysis of the data obtained, organizational solutions were developed with a change in the activities of a district nurse. An organizational experiment was conducted. The following methods were used: analytical, statistical, content analysis, organizational experiment.

Results. An analysis of regulatory legal acts showed that the functions of a district nurse include the implementation of a wide range of activities, but in practice many of them are not implemented. According to the survey results, the main functions of a district nurse were informing patients and inviting them to preventive measures (this function was noted by 90,8% of doctors and 99,0% of nurses) and preparing medical documentation at an appointment with a doctor (this function was noted by 85,5% of doctors, 94,3% of nurses and 45,4% of patients), which confirmed the results of observations. The organization of independent nursing appointments allowed to increase the effective time of the district nurse’s work with patients during an outpatient appointment from 14,2 to 49,5% and during a shift from 12,3 to 20,8%, as well as to reduce the functionally idle time during an outpatient appointment from 13,8 to 0% and during a shift from 10,7 to 2,4%.

Conclusion. Changes in the activities of the district nurse contribute to an increase in its importance in the formation  of  a  healthy  lifestyle  of  the  population,  in  the  prevention  of  noncommunicable  diseases  and  is a promising direction for improving primary health care. In matters of ensuring the availability of healthcare care, the professional competencies of the nursing staff should be taken into account.
Reproductive health promotion in working individuals. Guidelines
Authors: Drapkina O. M. , Orlov S. A. , Shepel R. N. , Kontsevaya A. V. , Apolikhin O. I. , Krasnyak S. S. , Dolgushina N. V. , Zalata I. V. , Lebedeva N. A. , Pobedennaya G. P. , Sharapova Yu. A.
Published: December 2024

The methodological recommendations have been developed with the aim of grading, systematizing and identifying key areas for the implementation of individual measures or a comprehensive corporate program for reproductive health promotion in working individuals at the employer level, including taking into account international experience.

The guidelines describe seven key subprograms aimed at early diagnosis and timely treatment of urinary tract diseases, assessment of reproductive health, improving literacy in sex education and behavior, reducing the negative impact of industrial and external environmental factors on reproductive health, increasing commitment to measures to combat risk factors for noncommunicable diseases, providing support during menopause in women and andropause in men, and creating conditions in the workplace conducive to maintaining a strong family. The guidelines are intended for healthcare and public health organization specialists, heads of state authorities of constituent entities of the Russian Federation in the field of health protection, specialists of regional centers for public health and medical prevention, as well as for heads of organizations in various economic sectors. The materials presented in the guidelines can also be used by medical workers of various specialties (paramedics, general practitioners (family doctors), obstetrics and gynecology doctors, dermatology and venereology specialists, medical prevention doctors, occupational pathology physicians and urologists).
Analysis of scientific sources for the presence and disclosure of the concept of hospitalization
Authors: Shepel R. N. , Savchenko D. O. , Naumov P. Yu. , Drapkina O. M.
Published: December 2024

Aim. To establish the essence, content and significant characteristics of the concept of hospitalization identified by various authors in publications from the electronic database Elibrary.ru, which is the basis of the Russian Science Citation Index.

Material and methods. The search was carried out taking into account the morphological forms of the semantic unit "hospitalization" in the titles of publications, abstracts, keywords of articles in journals, books, conference proceedings, deposited manuscripts, data sets, dissertations, reports, patents, grants published in the period from January 1, 2013 to December 14, 2024 and posted in the Elibrary.ru database. At the second stage, the received publications were assessed according to the inclusion and non-inclusion criteria. At the final stage, a detailed study of the found publications in the compiled sample was carried out for the presence and disclosure of the concept of hospitalization.

Results. In publications posted and summarized in the Elibrary.ru database, the concept of hospitalization is understood, as a rule, as the admission of a patient for medical examination and treatment in a medical organization in inpatient settings, including a day hospital.

Conclusion. Establishment of the substantive, structural and functional characteristics of the concept of hospitalization, which have been identified by various authors, is a pressing scientific challenge. The work identifies further promising areas of scientific research.
Apontamentos sobre a noção de território no campo da saúde coletiva: determinação, identidades e territorialidades
Authors: Prado Guilherme Augusto Souza , Caetano Maria Verônica Almeida
Published: December 2024

RESUMO Este ensaio teórico percorre um itinerário e aborda diferentes concepções de território quanto ao campo da saúde coletiva. Busca-se elucidar como o território compõe com a constituição histórica e organizativa dos sistemas e serviços de atenção à saúde, e como conceito fundamental ao trabalho no campo da saúde coletiva. Enquanto intervenção estatal sobre a vida populacional dentro de territórios circunscritos a Estados-nações, a abordagem epidemiológica paisagística da medicina social preventiva concebe o território como ambiente de exposição a doenças e acaba superada pelas ideias de risco e multicausalidade dos processos saúde-doença, próprias ao modelo do campo da saúde. Este amplia a compreensão dos processos saúde-doença articulando ambiente, acesso aos serviços, estilos de vida e determinantes biológicos com aspectos ecológicos, culturais, simbólicos e de responsabilidade social. Assim, a saúde coletiva se aprimora com noções como territorialidades, identidades ou reprodução social, importantes para a distritalização e a organização de sistemas de saúde democráticos, a compreensão sobre a determinação social da saúde e a efetivação de redes de cuidado e estratégias de assistência eficazes, sobretudo na atenção básica. Enfim, a noção de território é fundamental à compreensão das condições estruturantes dos processos de vida e adoecimento e ao compromisso com a transformação social.
Experiência formativa com uso do portfólio eletrônico como metodologia de ensino-aprendizagem
Authors: Silva Taís Gabriele Alves da , Almeida Isabella Silva de , Sousa Denise Lins de
Published: December 2024

RESUMO O Programa de Qualificação em Atenção Primária tem como objetivo fortalecer a Atenção Primária à Saúde no Distrito Federal, desenvolvendo ações de saúde proativas a partir da perspectiva de trabalhadores, gestores e representantes dos usuários do Sistema Único de Saúde. Em 2022, foi iniciado o Curso de Especialização em Gestão da Saúde da Família, em que se utilizou o portfólio eletrônico (e-Portfólio) como uma metodologia proativa, reflexiva e dialógica. Dentro de cada módulo, foram propostas intervenções para lidar com desafios cotidianos. A construção do e-Portfólio resultou em seis intervenções enfocadas na gestão da Estratégia Saúde da Família e alinhadas à qualificação do atendimento aos usuários. O uso desse recurso tecnológico gerou resultados positivos não apenas para a gestão, mas também para os funcionários, usuários e a comunidade. Além disso, facilitou o processo de feedback na jornada formativa, com a possibilidade de construção compartilhada, em que o professor-tutor acompanha a escrita, as reflexões, engaja-se com conteúdo teórico de apoio, aprimorando o processo de aprendizado e as mudanças nas práticas diárias.
Case study: coproduction of the Qualis-APS program to increase quality in Primary Health Care in Brasília
Authors: Santos Leonor Maria Pacheco , Scherer Magda Duarte dos Anjos , Furlanetto Denise de Lima Costa , Pedrosa Claudia Mara , Freitas Maria Silvia Fruet de , Leite Thais Alessa , Santos Wallace Enrico Boaventura Gonçalves Dos
Published: December 2024

ABSTRACT To encourage improvements in the quality of Primary Health Care in Brasília, the Federal District Health Department established the Qualis-APS Program in 2019, a co-production between healthcare professionals, managers and researchers from the University of Brasília and the Fundação Oswaldo Cruz. By involving managers and workers in the design, coordination, planning and execution, the aim was to promote engagement and a sense of ownership, prerequisites for evidence-based decision-making. The aim is to describe the conception and implementation of the evaluative process and the stages of the first evaluation cycle, concluded in 2022. I. Diagnosis of the structure of all 165 Basic Health Units; II. Elaboration of quality standards to compose self-assessment instruments, based on subsidies obtained in workshops with 544 health professionals and users; III. Development of the Qualis-APS Platform; IV. Self-assessment of the 603 Family Health Teams/Oral Health Teams, 100 Primary Health Care Management Teams and 59 Teams from the Expanded Family Health and Primary Care Centers on the Platform; V. Planning and elaboration of the Action Plan for Quality, made by the teams; VI. On-site evaluation of the Basic Units, by external researchers; VII. Certification of teams by the Health Department. The experience of the first cycle was successful and the second cycle is ongoing now.
Relato de experiência: coprodução do programa Qualis-APS para melhoria da qualidade da Atenção Primária à Saúde em Brasília
Authors: Santos Leonor Maria Pacheco , Scherer Magda Duarte dos Anjos , Furlanetto Denise de Lima Costa , Pedrosa Claudia Mara , Freitas Maria Silvia Fruet de , Leite Thais Alessa , Santos Wallace Enrico Boaventura Gonçalves Dos
Published: December 2024

RESUMO Para incentivar melhorias na qualidade da Atenção Primária à Saúde em Brasília, a Secretaria de Estado de Saúde do Distrito Federal (SES-DF) instituiu o Programa Qualis-APS em 2019: uma coprodução entre profissionais da assistência, gestores, pesquisadores da Universidade de Brasília e Fundação Oswaldo Cruz. Ao envolver gestores e trabalhadores na concepção, coordenação, planejamento e execução, almejou-se promover engajamento e senso de pertencimento, pré-requisitos para tomar decisões baseadas em evidências. Objetivou-se descrever a concepção e implementação da sistemática de avaliação e etapas do primeiro ciclo avaliativo, concluído em 2022: I. Diagnóstico da estrutura das 165 Unidades Básicas de Saúde; II. Elaboração de padrões de qualidade para compor instrumentos de autoavaliação, com base em subsídios obtidos nas oficinas com 544 profissionais de saúde e usuários; III. Desenvolvimento da Plataforma Qualis-APS; IV. Autoavaliação das 603 Equipes de Saúde da Família/Saúde Bucal, 100 equipes das Gerências da Atenção Primária à Saúde e 59 Equipes dos Núcleos Ampliados de Saúde da Família e Atenção Básica na Plataforma; V. Planejamento e elaboração do Plano de Ação para Qualidade pelas equipes; VI. Avaliação in loco das unidades básicas, por pesquisadores externos; VII. Certificação das equipes pela SES-DF. A experiência do primeiro ciclo foi exitosa; o segundo está em andamento.
Tipologia da estrutura das Unidade Básicas de Saúde do Distrito Federal
Authors: Furlanetto Denise de Lima Costa , Santos Wallace Enrico Boaventura Gonçalves dos , Cavalcante Fabrício Vieira , Xavier Mayra Fernandes , Lima Adriano de Almeida de , Santos Ricardo Ramos dos , Santos Leonor Maria Pacheco
Published: December 2024

RESUMO A tipologia da estrutura é um indicador composto que compreende a estrutura física, a disponibilidade de equipamentos, recursos materiais e humanos, além dos sistemas de informação. Com o objetivo de desenvolver uma tipologia para avaliar as Unidades Básicas de Saúde (UBS) do Distrito Federal (DF), construiu-se um instrumento com 43 variáveis, a partir da proposta de tipologia nacional. Entre agosto/2020 e janeiro/2021, coletaram-se dados nas UBS e nos sistemas de informação do DF. Na análise, ponderando as dimensões, gerou-se a classificação nos tipos A, B, C, D e E (A adequada/E insuficiente). Das 165 UBS existentes, 157 forneceram dados completos para avaliação. Três UBS obtiveram classificação Tipo A, máxima, representando 1,9% do total; 53 UBS do Tipo B (33,8%); 52 do Tipo C (33,1%); 30 do Tipo D (19,1%) e 19 do Tipo E (12,1%). Nove UBS apresentaram pontuação muito baixa em ‘serviços disponíveis’, expondo indisponibilidade de vacinação, teleatendimento, exames, medicamentos e incompletude de equipes. O resultado evidenciou que as UBS do DF necessitam de melhorias estruturais, que, em consonância com as demais ações previstas no Programa Qualis-APS, contribuirá para a melhoria da qualidade da atenção primária no DF e o desenvolvimento do pleno potencial das equipes de saúde.
Utilização dos relatórios do e-SUS da Atenção Primária à Saúde na rotina de trabalho de equipes de Saúde da Família
Authors: Passos Taciana Silveira , Alencar Tiago Machado de , Brito Alessandra Page , Sena Ayrton Martins Pereira , Santos Débora Bleza , Barreto Agilran Araújo , Silva Lídia Glasielle de Oliveira
Published: December 2024

RESUMO Objetivou-se analisar a utilização dos relatórios do e-SUS da Atenção Primária à Saúde (APS) na rotina de trabalho das equipes de Saúde da Família (eSF) do Distrito Federal (DF). Estudo descritivo de dados provenientes da Avaliação in loco e Plano de Ação para a Qualidade do 1º ciclo avaliativo do Programa de Qualificação da Atenção Primária à Saúde, entre 2021 e 2022. Incluíram-se todas 607 equipes. Associações entre as Regiões de Saúde foram identificadas mediante testes Qui-quadrado, p-valor < 0,05. Para quantificar problemas e ações, utilizaram-se técnicas de Mineração de Dados. A maioria das eSF do DF negaram o uso de qualquer relatório do e-SUS APS em sua rotina de trabalho (53,23%). Os relatórios que as eSF mais utilizaram foram os de produção (54,43%), e menos, os de gerenciais (31,60%). As regiões com maior proporção de uso foram Centro-Sul (73,67%), e com menor, Sudoeste (34,56%), p < 0,0001. Alguns dos principais problemas descritos foram intrínsecos ao e-SUS APS (erros, falhas, instabilidade do sistema e falta de unificação) e outros foram relativos a questões extrínsecas (desconhecimento, inconsistência e falta de registro). As equipes listaram em seus Planos de Ação, prioritariamente, as capacitações para uso correto do sistema de informação.
Benefícios das Práticas Integrativas e Complementares em Saúde na qualidade de vida e nos sintomas de mulheres no climatério: uma revisão sistemática
Authors: Sobral Bárbara Angélica Bispo Fernandes de Nascimento , Silva Rafaela Almeida , Souza Claudinalle Farias Queiroz de , Abrão Fátima Maria da Silva , Costa Aurélio Molina da
Published: December 2024

RESUMO Existem muitas intervenções terapêuticas à mulher em fase de climatério. As Práticas Integrativas e Complementares em Saúde (Pics), embora não sejam amplamente utilizadas, apresentam um bom custo- -benefício e são coerentes com os conceitos de autocuidado e de saúde integrativa, sendo relevante avaliar suas beneficências. Objetivou-se descrever e sintetizar as evidências científicas disponíveis na literatura sobre os benefícios dessas práticas para a assistência à mulher em fase de climatério. Fez-se uma revisão sistemática construída a partir da busca de ensaios clínicos que respondessem à seguinte pergunta de pesquisa: quais os benefícios das Pics na melhoria da qualidade de vida e dos sintomas de mulheres no climatério? Realizou-se uma busca nas bases de dados Cumulative Index to Nursing and Allied Health, Latin American and Caribbean Health Sciences Literature, Embase e Medical Literature Analysis and Retrieval System Online. Como resultado, identificaram-se 24 estudos que atenderam aos critérios de inclusão e ao protocolo PRISMA. Todos os ensaios clínicos da amostra final investigaram apenas 3 entre as 29 Pics - acupuntura, yoga e fitoterapia - ofertadas pelo Sistema Único de Saúde. A revisão realizada sugere que a yoga, a acupuntura e a fitoterapia, com diferentes extratos de ervas, têm lugar na assistência integrativa das mulheres no climatério.
Autoevaluación De Habilidades Socio-Comunicativas En Simulación Odontológica: Criterios Clave Para La Formación Profesional: Scoping Review
Authors: Trigari Saravia Nabor Ignacio , Leiva Zuñiga Mario Andres Leiva Zuñiga , Espinosa San Martín Marcela
Published: December 2024

La formación odontológica requiere habilidades técnicas y sociocomunicativas, esenciales para un ejercicio profesional eficaz y empático. Para desarrollarlos, el uso de la simulación en hogares y pacientes estándar es una herramienta eficaz e innovadora. Sin embargo, la percepción de los estudiantes a través de las autoevaluaciones es crucial para retroalimentar y mejorar estas habilidades. Este estudio tiene como objetivo identificar criterios clave para desarrollar un instrumento de autoevaluación que mida la percepción de habilidades sociocomunicativas en escenarios simulados, a través de una revisión exploratoria (Scoping Review).
Formulamos la pregunta de investigación, siguiendo el formato PCC: “¿Cuáles son los criterios clave para medir la percepción de habilidades sociocomunicativas a través de la autoevaluación en simulaciones odontológicas?”. Diseñamos una estrategia de búsqueda estandarizada en PubMed y Scopus. Los artículos seleccionados incluyeron autoevaluaciones relacionadas con habilidades sociocomunicativas en programas de pre y posgrado en ciencias de la salud, sin límite de año de publicación. Se excluyeron artículos no relacionados con habilidades sociocomunicativas y aquellos que no contienen una agenda autoevaluativa. Analizamos 1.306 artículos, de los cuales seleccionamos 16 que eran relevantes, utilizando para el informe la guía PRISMA ScR.
Los resultados mostraron que el 100% de los artículos seleccionados abordaron autoevaluaciones en habilidades sociocomunicativas, agrupamos los criterios en cinco áreas principales: comunicación verbal, comunicación paralingüística, comunicación no verbal, establecimiento de metas y preguntas de autopercepción. Las cinco áreas identificadas permitirán una evaluación integral y estructurada de las habilidades de los estudiantes en contextos simulados.
CHA2DS2-VASc Score for Predicting the Risk of Contrast-Induced Nephropathy After Transcatheter Aortic Valve Replacement
Authors: Özkan Can
Published: December 2024

Contrast induced nephropathy (CIN) is a condition that can develop due to percutaneous cardiac interventional procedures and can adversely affect the prognosis of patients. The CHA2DS2-VASc score has been shown to be an independent predictor of acute kidney injury (AKI) and indicates an unfavorable prognosis in patients with acute coronary syndrome. Knowledge of the risk of AKI prior to transaortic valve replacement (TAVR) may help to reduce the rate of AKI. Therefore, we aimed to evaluate the utility of CHA2DS2 VASC score in patients undergoing TAVR.
Comparison of Fluoroscopy Reducing OLIF51 and Standard TLIF51 Methods in the Surgical Treatment of Adult Spinal Deformity
Authors: Eryılmaz Fahri , Yurdakul Göker
Published: December 2024

Objective: The objective of the study was to evaluate the OLIF51 (L5-S1 Oblique Lumbar Interbody Fusion) and TLIF51 (L5-S1 Transforaminal Lumbar Interbody Fusion) techniques in relation to several key variables, including the duration of the operation, quantity of blood lost, fluoroscopy time used, radiological outcome and complications as regards the management of adult spinal deformity.
Materials and Methods: In this retrospective study, a total of 60 patients who underwent surgical treatment for spinal deformities between January 2018 and December 2023 were included in the analysis. Patients were divided into two groups according to OLIF51 and TLIF51 methods. Demographic data, surgical time, blood loss, hospitalization, fluoroscopy time, and complication rates were recorded. VAS (Visual Analog Scale) and ODI (Oswestry Disability Index) were used for clinical evaluation. Radiological parameters were analyzed using disc height and lumbar lordosis angle.
Results: The OLIF51 group exhibited a shorter surgical duration (p < 0.001), reduced blood loss (p < 0.001), and a shorter hospital stay (p = 0.002) compared to the control group. Moreover, the OLIF51 procedure demonstrated a notable reduction in the utilization of fluoroscopy, with a statistically significant difference (p < 0.001). In terms of radiological outcomes, the OLIF51 group exhibited superior disc height and lumbar lordosis angle measurements compared to the TLIF51 group, with statistical significance (p < 0.05). No significant difference was observed between the two groups in terms of clinical improvement.
Conclusion: The OLIF51 procedure offers several advantages over TLIF51, including a shorter operative time, reduced blood loss, and less fluoroscopy time. Additionally, radiological outcomes are more favorable for OLIF51. For this reason, OLIF51 can be regarded as a secure and effective alternative for the management of spinal deformities.
Coral Reef Restoration for Coastal Protection: Water Quality Monitoring in Indonesia Coral Reef Garden Nusa Dua, Bali
Authors: Burhanuddin Safri , Badriana Mochamad Riam , Nur Ashadi Arifin , Abdurrahman Umar , Jeon Chungkyun , Radjawane Ivonne Milichristi , Park Hansan , Hutahaean Andreas Albertino
Published: December 2024

The Indonesia Coral Reef Garden (ICRG) is a coral reef restoration program with Nusa Dua as one of five sites in Bali. A plantation program has been done by 2020, but it is necessary to monitor the condition. A field survey in Nusa Dua was carried out on October 3-4, 2022 to measure and check ocean parameters that are suitable for coral life and diving in artificial coral reefs installed. In the survey, several points had been measured through CTD Valeport Midas+ prior to measuring ocean parameters in the same location as a preliminary survey done two years in advance. The sea condition of temperature, salinity, pH, turbidity, and DO is 24.89 - 28.69 °C, 33.75 - 34.22 psu, 8.0 - 8.1, 1.19 - 4.27 FTU, and 4.47 - 5.24 mg/L, respectively. Based on the conformity of water quality, the water condition is good for coral growth. A total of 15 locations are used to put artificial coral reefs in many forms or structures. The coral plantation program in Nusa Dua shows promising progress in qualitatively consisting of species of Montipora sp., Acropora sp., and Alcyonacea sp. A continuous measurement is demanded to monitor the condition of coral growth quantitatively.
Dispersed hardening by TiB2 particles of SiC ceramic material
Authors: Antonova E. S. , Golubeva N. A. , Lisachenko M. G.
Published: December 2024

Particle-reinforced SiC composites with the addition of TiB2 were fabricated by hot-pressing. Their mechanical properties were measured as a function of TiB2 content. Adding TiB2 to the SiC matrix increased the fracture toughness and the flexural strength at the room temperature. The fracture toughness and the flexural strength at the room temperature of SiC‒30 % wt. TiB2 composite were approximately 72 and 43 %, respectively, higher than that of monolithic SiC ceramics.
XXI International conference for refractorists and metallurgists
Authors: Gavrik G. G.
Published: December 2024

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Manufacturing of tool electrodes with optimized configuration for copy-piercing electrical discharge machining by rapid prototyping method
Authors:   Perm National Research Polytechnic University, Russian Federation, Ablyaz Timur , Blokhin Vladimir ,   Perm National Research Polytechnic University, Russian Federation, Shlykov Evgeniy ,   Perm National Research Polytechnic University, Russian Federation, Muratov Karim ,   Perm National Research Polytechnic University, Russian Federation, Osinnikov Ilya ,   Perm National Research Polytechnic University, Russian Federation
Published: December 2024

Introduction. This paper presents the results of obtaining a complex-profile tool electrode (TE) for copy-and-pierce electrical discharge machining by casting technology. This method consists in using a master model by rapid prototyping method. The purpose of the work: experimental study of accuracy assurance in manufacturing of complex-profile TE by casting with the use of rapid prototyping technology for copy-piercing electrical discharge machining. Research Methods. The master model of TE was produced on the Envisiontec Perfactory XEDE machine using stereolithography technology. Si500 photopolymer was used as a starting material. Intermediate and final surface deviation measurements were performed on a Contura Carl Zeiss G2 CMM. Calculation of the gutter and feed system was performed in ProCast software. A casting was obtained from casting brass LC40S (Cu-40 Zn-Pb). The study of the process of copy-piercing electrical discharge machining of TE made by casting with the use of rapid prototyping technology was carried out with the help of Smart CNC copy-piercing machine in the environment of transformer oil. Operating parameters: pulse turn-on time (Ton, μs), voltage (U, V), current (I, A). Results and discussion. The methodology of design and manufacturing of complex-profile TE with application of rapid prototyping technology for copy-piercing electrical discharge machining is developed. The analysis of shape deviation shows that errors occur during the manufacturing of the master model by stereolithography. An experimental study of the shape deviation of the master model shows surface concavity in the range of 0.03 to 0.07 mm depending on the arrangement of the sides. It is shown that the optimized master model has 25 % less shape deviation. A sprue-feeding system (SFS) is developed for the fabrication of TE by casting technology. When porosity is evaluated, it is found that pores are concentrated in the SFS and riser, which positively affects the quality of the casting. Manufacturing of the tool electrode with the help of casting technology showed that all accuracy and roughness parameters are within the specified tolerance and correspond to the initial drawing data. Experimental study of the process of electroerosion machining of the profile groove of the TE manufactured by casting on the investment casting model obtained with the use of rapid prototyping technology is carried out. It is established that the dimensions of the obtained groove meet the stated requirements.
JAX-Sen:Chromium Fixed RNA Profiling (CG000527 Revision F) v1
Authors: L. Daigle Sandra
Published: December 2024

10x Genomics Chromium Chromium Fixed RNA Profiling.
Paraffin section tissue preparation v1
Authors: Seifert Ashley
Published: December 2024

This protocol describes the procedure used by the Seifert Lab to prepare paraffin-embedded tissue sections.
shRNA Design, Subcloning, and Lentivirus Production v1
Authors: Kumar Mukesh
Published: December 2024

This protocol provides a detailed method for designing, subcloning, and producing shRNA-expressing lentivirus for gene silencing experiments in primary cultured hippocampal neurons.
Amplification and sequencing of HTLV-1 whole-genome using Nanopore v1
Authors: Lopes da Silva Amanda , Morales Ingra , Gomes Catozo Raquel , Luiz Miranda Guedes Bruno , Santos de Souza Caio , Santos Andrade Pâmela , Pereira Geovana , Simão do Rosário Casseb Jorge , Amorim Mariene , M Romano Camila
Published: December 2024

Human T-lymphotropic virus type 1 (HTLV-1) remains frequently underrecognized despite its association with severe diseases in 3–5% of infected individuals. This lack of awareness, coupled with the limited availability of complete HTLV-1 genome sequences in public databases, impairs progress in understanding the virus. Current sequencing techniques, such as Sanger and Illumina, are often costly, and/or time-consuming. Expanding our knowledge of HTLV-1’s genomic structure is crucial for advancing our understanding of its basic biology, improving epidemiological studies, and investigating how genetic variation contributes to the diverse clinical manifestations of HTLV-1-associated diseases. To address these gaps, we developed an efficient and cost-effective method for amplifying and sequencing the complete HTLV-1 genome using Nanopore technology on the MinION platform. This approach offers significant advantages, including real-time sequencing, portability, and relatively low cost compared to traditional methods. Our protocol can facilitate studies on HTLV-1's genetic diversity and pathogeny.
Protocol for Investigating Neuronal Communication via Gap Junctions v1
Authors: Zhu Wenjie
Published: December 2024

This protocol established a DRG communication system to study axonal communication through GJs. SARM1, an NAD-consuming enzyme, regulates axonal degeneration. Using the SARM1 activity-detecting probe PC11, we found that intercellular communication may regulate SARM1 activation, protecting against axonal degeneration. A related study utilizing this protocol has been published in Cell Death and Disease. This novel approach offers a streamlined method for researches of sensory neuron and GJs intercellular communication among neurons, with potential for advancing our understanding of sensory disorders and developing targeted therapies.
Shake-Flask Aqueous Solubility assay (Kinetic solubility) v1
Authors: Lesyk Dmytro , Kheilik Yurii
Published: December 2024

Determining compound solubility is an essential tool for the early stages of the drug discovery process, as well as for lead optimization. Low solubility can lead to unpredictable and unreliable results during in vitro testing, thereby increasing the development costs. Solubility issues at the later stages of the drug discovery may lead to poor bioavailability, underestimated toxicity, and other obstacles, lowering the chances of a given drug candidate for success. Typically, for early-stage drug discovery, the kinetic solubility method is used, as it is fast and well-suited for the HTS format. In this case, solid compounds are first dissolved in DMSO, and then linear serial dilutions of each compound are added to an aqueous buffer and observed for precipitate formation when the compound is not completely soluble. For better precision, the solution can be subjected to high-speed centrifugation or filtration using special solubility filter plates and then the compound concentration is measured in the saturated solution directly by UV or LC-MS/MS using separately built calibration curves. Measurements were performed by the shake-flask method with UV-Vis or LC-MS/MS detection.
In vitro organ culture of intact urogenital systems supporting gonadal differentiation v1
Authors: Whiteley Sarah , Holleley Clare , Georges Arthur
Published: December 2024

This protocol is for the in vitro culture of intact embryonic urogenital systems using an agarose substrate. This protocol has been optimised for the model reptile species, Pogona vitticeps, but could be adapted for other species. This protocol supports gonad differentiation from bipotential to ovaries or testes that are distinguishable morphologically and by gene expression patterns. Gross morphology is well maintained even during the longest culture duration (19 days) such that the gonads can be dissect from the surrounding mesonephros, improving utility for downstream sequencing applications.
Western blotting using NuPAGE system v1
Authors: Seifert Ashley
Published: December 2024

This protocol describes the procedure for western blotting with the NuPAGE system used by the Seifert Lab. It supplements the manufacturer's guide to the NuPAGE electrophoresis system: https://tools.thermofisher.com/content/sfs/manuals/nupage_tech_man.pdf
Assessing Intestinal and CNS Permeability with MDR1-MDCKII Cells v1
Authors: Rodnichenko Anzhela , Kheilik Yurii
Published: December 2024

The protocol allows measurement of the rate of flux of a compound to identify intestinal or CNS permeability. The assay measures the rate of flux across Canine MDR1 Knockout, Human MDR1 Knockin MDCKII cells (MDR1-MDCKII) cell monolayers to identify intestinal or CNS permeability. By measuring how quickly a compound passes through MDR1-MDCKII cell monolayers, researchers can estimate its intestinal permeability and predict its potential to reach the brain. Interpreting Results: High Permeability: A compound that readily crosses the MDR1-MDCKII monolayer is likely to be well-absorbed from the intestine and may have a high potential to reach the brain. Low Permeability: A compound that does not readily cross the monolayer may have limited intestinal absorption or be actively pumped out of the brain by the MDR1 protein.
Reseña de Giri, L., Melogno, P., Miguel, H. (2023) Perspectives on Kuhn. Contemporary Approaches to the Philosophy of Thomas Kuhn – Springer
Authors:   Universidad de Concepción, Núñez-Pradenas Rolando
Published: December 2024

Este texto es una reseña crítica del libro Perspectives on Kuhn. Contemporary Approaches to the Philosophy of Thomas Kuhn de Giri, L., Melogno, P., Miguel, H. (2023)
Morphological and biochemical characterization of Moroccan Opuntia dillenii fruit: Natural source of bioactive compounds
Authors:   Université Sultan Moulay Slimane, Zazouli Sofia , Kabiri Ghizlane ,   Université Sultan Moulay Slimane
Published: December 2024

Background: The fruit of Opuntia dillenii, commonly known as prickly pear, is widely consumed for its nutritional and medicinal benefits, such as anti-inflammatory and antioxidant properties. It plays a significant role in local diets and traditional medicine, contributing to economic value. However, research on its phytochemical composition and health benefits is limited, highlighting the need for further investigation.
Objectives: The current study assessed morphological traits, biochemical composition, and antioxidant activity using the 2,2-diphényl-1-picrylhydrazyl (DPPH) assay.
Methods:The morphological traits, biochemical parameters, and antioxidant content of the Moroccan O. dillenii fruit fractions, namely peel, juicy pulp, and seeds, were investigated.
Results: The coefficient of variation of the morphological traits oscillated between 16.03 % for fruit weight and 51.83 % for seed weight, indicating a broad level of morphological variability. The total phenolic content of fruit fractions ranged from 202 to 56 g EAG/ 100 g extract, while the total flavonoids varied from 185 to 11 mg EC/100g extract. In addition, the total levels of betacyanins, betaxanthins, and condensed tannins ranged from 6.1 to 335 mg/L, 4.7 to 123 mg/L, and 12 to 8.3 mg/100g, respectively. As for ascorbic acid, it was concentrated in the juicy pulp at 580 mg/100 g, while it was absent in the seeds fraction. The phenolic compounds, flavonoids, and betalain contents were significantly correlated with antioxidant activities, whereas total ascorbic acid and condensed tannins were weakly correlated.
Conclusion: These findings suggest that O. dillenii fruits may be a potential source of natural antioxidants for both food applications and medicinal functions.
Temporalities of Arrival: Burundian Barbershops as an Arrival Infrastructure in a South African Township
Authors: Mbatha Nomkhosi A. , Koskimaki Leah
Published: December 2024

Migrants from Sub-Saharan Africa have contributed to the diversity of the informal economy in urban South Africa. However, they have faced xenophobic violence and discrimination in urban spaces such as townships, which were previously designated only for Black people during apartheid. This article explores how arrival infrastructures in the township informal economy have enabled or hindered economic opportunities for those who have newly come to South Africa. Based on qualitative research on practices of solidarity and conviviality with migrant informal traders from Burundi, the Democratic Republic of Congo, and Malawi in the township of Umlazi in Durban, South Africa, in 2023, the article specifically focuses on Burundian migrants’ barber businesses as a node in the arrival infrastructure. These barbershops act as informal social spaces that provide access to “connectors” who help with networks to acquire labour, social, and material resources. These include local knowledge and information about new locations to construct a barber business or introductions to property owners. This article argues, however, that informal market and business spaces are often temporary for migrants who are always on the move, continually arriving and re-negotiating their belonging due to multiple waves and threats of xenophobic violence. Therefore, the barber business represents a temporary structure for futuring in uncertain times.
Marginal ulcer causing anastomotic perforation and delayed penetration following one anastomosis gastric bypass
Authors: Chaykin Aleksandr Aleksandrovich , Chaykin Dmitriy Aleksandrovich , Vinnik Yurii , Teplyakova Olga , Ilinov Aleksandr , Boyakova Nina
Published: December 2024

We report the rare clinical case of perforation and delayed penetration of an ulcer at the gastro-jejunal anastomosis following 14 months after laparoscopic one anastomosis gastric bypass in a young female patient. No significant ulcer predictors at baseline: a chronic ulcer formed within the first six months after one anastomosis gastric bypass against the background of continued proton pump inhibitor therapy, regardless of smoking, helicobacter infection or use of non-steroidal anti-inflammatory drugs. Initially the patient underwent the laparotomy with perforation suturing and Roux-jejunojejunostomy formation to exclude the bile reflux into the gastric pouch. The result of the first surgery (non-healing ulcer with the development of delayed penetration six months later) is agree with the recent literature about the possible overstatement of the role of biliary reflux in ulcer formation after one anastomosis gastric bypass. The follow-up surgery was performed by the laparoscopic approach in condition of pronounced adhesion process in abdominal cavity and included adhesiolysis, resection of the gastric pouch with the anastomosis and reconstructive Roux-Y-gastric bypass. Clinical and endoscopic evaluation of the result of the reconstructive surgery 10 months after indicates the patient's recovery.
Using MOOCs to organise problem-based learning in the professional training of IT students
Authors: Spirina Ye. A. , Gorbunova N. A. , Samoilova I. A.
Published: December 2024

Introduction. The digital transformation of education plays a key role in the formation of in-demand IT specialists. In modern conditions, the method of problem-oriented learning is actively being introduced into the educational process of higher education and contributes to the formation of professional, communicative and digital competencies of IT students.

Aim. The present research aimed to validate the incorporation of massive open online course (MOOC) components in the organisation of problem-oriented training for IT students to enhance their professional competencies.

Methodology and research methods. The study was conducted over two academic years, beginning in 2022, at Academician E. A. Buketov Karaganda University. A total of 24 teachers participated in the specialised training of IT students, along with 86 students participated. At the first stage, a model was developed to integrate MOOCs into the structure of a specialised IT discipline within the framework of a specialised IT discipline, utilising problem-oriented learning. Additionally, the baseline level of students’ readiness to engage with MOOC courses was assessed. At the second stage, the effectiveness of utilising MOOC courses for implementing problem-oriented learning in the study of IT disciplines was assessed. At the third stage, the results were summarised and conclusions were drawn.

Results and scientific novelty. The approaches to the organisation of problem-based learning in the preparation of IT students were defined. The use of Coursera MOOCs as the most convenient platform for studying IT courses was justified. A model of MOOC integration into the structure of an academic discipline in the organisation of problem-oriented learning was developed and experimentally tested.

Practical significance. The results of an empirical study of student involvement in the use of MOOCs in problem-based learning prove the functionality of Coursera MOOC courses and their positive impact on the learning process. The feasibility of utilising MOOCs for implementing problem-oriented training aimed at developing students’ professional IT competencies is well-supported.
Development of the PLONG learning model as an innovation to develop communication and collaboration skills
Authors: Estimurti E. S. , Pantiwati Y. , Latipun L. , In’am A. , Huda A. M. , Bulkani B.
Published: December 2024

Introduction. Communication and collaboration skills are very important skills in learning. These skills help students achieve learning success; however, many students have low collaboration and communication skills in schools.

Aim. The present research aimed to demonstrate the potential of the PLONG (Playing, Learning, Doing, and Evaluating) learning model in developing students’ communication and collaboration skills.

Methodology and research methods. This research applied the ADDIE model, which encompasses needs analysis, model design, material and activity development, field implementation, and evaluation. The study involved 101 students and 10 classroom teachers as participants. The instruments employed included model feasibility assessments, a PLONG model group discussion forum, and a questionnaire measuring collaboration and communication skills. Data analysis incorporated both qualitative and quantitative methods.

Results and scientific novelty. It has been established that the PLONG learning model effectively enhances students’ communication and collaboration skills. These results are supported by an analysis comparing preand post-intervention data. This model increases students’ motivation and readiness to work collaboratively in teams. The novelty of this study lies in the fact that the PLONG learning model is regarded as a comprehensive approach to education, allowing students to independently assess their learning outcomes during the process, which includes engaging game elements. Additionally, PLONG offers feedback that aids students in their improvement.

Practical significance. With the PLONG model, students engage in play, learning, action, and assessment, fostering an active and enjoyable learning environment that promotes the optimal development of collaboration and communication skills.
Symmetry-breaking Indeterminism as a Challenge to Generalism
Authors: Bigaj Tomasz
Published: December 2024

The debate between individualism and generalism (qualitativism) concerns, inter alia, the question of whether qualitative identity implies numerical identity. One important argument against the affirmative answer to this question is based on an analysis of cases known as symmetry-breaking indeterministic processes, including the case of a collapsing tower. Generalism implies that all processes of that kind are deterministic, contrary to our snap judgment. This article contains a multifaceted analysis of the above argument against generalism, taking into account its metaphysical, scientific and modal aspects. It is suggested that the intuition of indeterminism regarding the considered cases can be explained by a distinct phenomenon of indeterminacy affecting the diachronic identity of participating objects. It is also observed that both individualism and generalism are unable to formulate a theory which could make definite predictions that are more specific than the ones made in purely qualitative language. This strongly suggests that the purported indeterminism of the considered scenarios is not genuine. Finally, the modal analysis shows how generalism is capable of expressing the idea of alternative possibilities without violating the fundamental assumption of determinism. This approach is based on the Lewisian counterpart semantics for quantified modal logic with same-world counterparts.
Lewis on Existence and Actual Existence
Authors: Hughes Christopher
Published: December 2024

David Lewis holds that only some of the things that exist actually exist. He concedes that this is, on the face of it, an incredible view, but he argues that if we do enough philosophy, we can come to see that it is true. I examine and evaluate Lewis’s arguments to that effect.
Sistema de cuentas económicas distritales de Santiago de Cali y la nueva cuenta satélite de economía cultural y creativa
Authors:   Universidad Icesi, Alonso Cifuentes Julio César , Castaño Astaiza Óscar Andrés ,   Universidad Icesi, Chavarriaga Antonio Viviana ,   Universidad Icesi, Chaves Ocampo Cristian Javier ,   Universidad Icesi, Osorio Colonia Juan Camilo ,   Universidad Icesi, Quijano Colonia Maicol Leandro ,   Universidad Icesi, Sarmiento García Víctor Manuel ,   Universidad Icesi, Serrano Izquierdo Estefanía ,   Universidad Icesi
Published: December 2024

El Producto Interno Bruto (PIB) es la herramienta más reconocida por analistas económicos para sintetizar el nivel de actividad económica en una región o país. En el contexto del Distrito de Santiago de Cali, el Sistema de Cuentas Económicas Distritales (SCED) permite una medición descentralizada del PIB tanto de manera trimestral como anual. Desde su primera implementación en 2004, este sistema ha evolucionado y se ha ajustado con el objetivo de ofrecer la representación más precisa posible del PIB para Santiago de Cali. En esta publicación, compartimos la última actualización del SCED y sus resultados más recientes. El SCED de Cali sigue los lineamientos de la metodología de cuentas nacionales de Naciones Unidas, adaptándola a las particularidades del Distrito. Esto permite que los resultados de este ejercicio estadístico no solo brinden una imagen detallada de la estructura económica de Cali, sino también ofrecer una herramienta valiosa para la planificación y la toma de decisiones a nivel económico y social. El presente libro recoge los resultados de un convenio de asociación entre el DAP y el Cienfi, cuyo propósito es actualizar las cuentas macroeconómicas anuales y trimestrales de Cali. Esta edición presenta los resultados de emplear la metodología del SCED para el año 2023 e introduce el cálculo de la nueva Cuenta Satélite de Economía Cultural y Creativa (CSECC) siguiendo el marco del SCED.