Monitoring of biochemical indicators of blood after surgical interventions on the digestion apparatus in rabbits
Authors: Kovalenko D. O. , Malyuk M. O. 
Published: May 2024

This article presents an overview of the results of modern scientific research devoted to monitoring biochemical parameters of rabbits' blood after operative interventions on the digestion apparatus. The purpose of the study is to assess the effectiveness of monitoring the biochemical indicators of the blood of rabbits after surgical interventions on the stomach. The analysis was carried out using chemical reactions aimed at the interaction of specific reagents with these molecules, which made it possible to accurately determine their amount in the blood serum of rabbits. Blood serum was obtained by centrifugation on an Eppendorf 5702R centrifuge (Germany) for 4 minutes at 4000 revolutions per minute and was subjected to further investigation on an automatic biochemical analyzer, Mindrey BS240 (China). The results obtained were analyzed to determine the statistical significance of the values. Mean value, standard deviation, and p-value calculation methods were used to assess the degree of reliability of the obtained results. It was scientifically confirmed that in the animals of the first and second experimental groups, the glucose content decreased relative to the initial state within 10 days after the surgical intervention. On the fourteenth day, the glucose level was restored to the initial state. It should be noted that the level of glucose in the animals of the first and second experimental groups did not exceed the physiological parameters. It was established that the calcium and phosphorus content of the animals of the first experimental group did not exceed the physiological norm after the surgical intervention on the stomach. The potassium content exceeded the physiological parameters on the first, third, seventh, and tenth days after surgery. On the fourteenth day after surgery, the potassium content was restored to the initial level, as in the second group of subjects. In the animals of the second experimental group, the calcium and phosphorus content after surgical intervention on the stomach had slight fluctuations but did not go beyond the physiological parameters. Potassium content increased after gastric surgery and exceeded physiological parameters on the first, third, seventh, tenth, and fourteenth days after surgery. The content of renal indicators of creatinine and urea in the animals of the first experimental group increased beyond the physiological parameters on the third, seventh, tenth, and fourteenth days after surgery. In the animals of the second experimental group, in turn, the content of indicators of kidney function after gastric surgery exceeded the initial state on the first, third, seventh, and tenth days of the experiment; on the fourteenth day, the creatinine content was higher than the initial state indicator but corresponded to the physiological norm. The total protein and albumin content decreased in the rabbits of the first and second research groups after surgery throughout the entire research period. The activity of AST, ALT, GHT, and alkaline phosphatase enzymes in the animals of the first and second experimental groups was higher than the baseline values during the entire research period. The findings of the study indicate that the first group shows better results, confirming the feasibility of using additional medical drugs (in particular, antibiotic therapy with enroxyl at a dose of 5 mg/kg once a day for seven days, the use of prokinetic agents such as metoclopromide at a dose of 1 mg /kg every 12 hours for three days, and analgesia the patients received butorphanol at a dose of 0.1 mg/kg every 6 hours for seven days and infiltration of platelet-rich plasma intraoperatively at the site of the gastric incision ), although the second group also received treatment, according exclusion of platelet-rich plasma.