bluuebunny/crossref_metadata_2025 huggingface dataset:
Network traffic analyzing algorithms on the basis of machine learning methods
Authors:   Ufa State Aviation Technical University, K. Marks str., 12, Ufa, Russia, 4500082, Battalov Radmir , Nikonov Andrey ,   Ufa State Aviation Technical University, K. Marks str., 12, Ufa, Russia, 4500082, Gayanova Maya ,   Ufa State Aviation Technical University, K. Marks str., 12, Ufa, Russia, 4500082, Berkholts Viktoriya ,   Ufa State Aviation Technical University, K. Marks str., 12, Ufa, Russia, 4500082, Gayanov Ruslan ,   Higher School of Economics, Myasnitskaya str., 20, Moscow, Russia, 101000
Published: December 2019

Traffic analysis systems are widely used in monitoring the network activity of users or a specific user and restricting client access to certain types of services (VPN, HTTPS) which makes content analysis impossible. Algorithms for classifying encrypted traffic and detecting VPN traffic are proposed. Three algorithms for constructing classifiers are considered - MLP, RFT and KNN. The proposed classifier demonstrates recognition accuracy on a test sample up to 80%. The MLP, RFT and KNN algorithms had almost identical performance in all experiments. It was also found that the proposed classifiers work better when the network traffic flows are generated using short values of the time parameter (timeout). The novelty lies in the development of network traffic analysis algorithms based on a neural network, differing in the method of selection, generation and selection of features, which allows to classify the existing traffic of protected connections of selected users according to a predetermined set of categories.
Farnesyl diphosphate synthase; regulation of product specificity.
Authors: Szkopińska Anna , Płochocka Danuta
Published: December 2019

Farnesyl diphosphate synthase (FPPS) is a key enzyme in isoprenoid biosynthesis which supplies sesquiterpene precursors for several classes of essential metabolites including sterols, dolichols, ubiquinones and carotenoids as well as substrates for farnesylation and geranylgeranylation of proteins. It catalyzes the sequential head-to-tail condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate. The enzyme is a homodimer of subunits, typically having two aspartate-rich motifs with two sets of substrate binding sites for an allylic diphosphate and isopentenyl diphosphate per homodimer. The synthase amino-acid residues at the 4th and 5th positions before the first aspartate rich motif mainly determine product specificity. Hypothetically, type I (eukaryotic) and type II (eubacterial) FPPSs evolved from archeal geranylgeranyl diphosphate synthase by substitutions in the chain length determination region. FPPS belongs to enzymes encoded by gene families. In plants this offers the possibility of differential regulation in response to environmental changes or to herbivore or pathogen attack.
Isolation of Nicotiana plumbaginifolia cDNAs encoding isoforms of serine acetyltransferase and O-acetylserine (thiol) lyase in a yeast two-hybrid system with Escherichia coli cysE and cysK genes as baits.
Authors: Liszewska Frantz , Gaganidze Dali , Sirko Agnieszka
Published: December 2019

We applied the yeast two-hybrid system for screening of a cDNA library of Nicotiana plumbaginifolia for clones encoding plant proteins interacting with two proteins of Escherichia coli: serine acetyltransferase (SAT, the product of cysE gene) and O-acetylserine (thiol)lyase A, also termed cysteine synthase (OASTL-A, the product of cysK gene). Two plant cDNA clones were identified when using the cysE gene as a bait. These clones encode a probable cytosolic isoform of OASTL and an organellar isoform of SAT, respectively, as indicated by evolutionary trees. The second clone, encoding SAT, was identified independently also as a "prey" when using cysK as a bait. Our results reveal the possibility of applying the two-hybrid system for cloning of plant cDNAs encoding enzymes of the cysteine synthase complex in the two-hybrid system. Additionally, using genome walking sequences located upstream of the sat1 cDNA were identified. Subsequently, in silico analyses were performed aiming towards identification of the potential signal peptide and possible location of the deduced mature protein encoded by sat1.
Rsp5 ubiquitin ligase affects isoprenoid pathway and cell wall organization in S. cerevisiae.
Authors: Kamińska Joanna , Kwapisz Marta , Grabińska Kariona , Orłowski Jacek , Boguta Magdalena , Palamarczyk Grazyna , Zoładek Teresa
Published: December 2019

Dimethylallyl diphosphate, an isomer of isopentenyl diphosphate, is a common substrate of Mod5p, a tRNA modifying enzyme, and the farnesyl diphosphate synthase Erg20p, the key enzyme of the isoprenoid pathway. rsp5 mutants, defective in the Rsp5 ubiquitin-protein ligase, were isolated and characterized as altering the mitochondrial/cytosolic distribution of Mod5p. To understand better how competition for the substrate determines the regulation at the molecular level, we analyzed the effect of the rsp5-13 mutation on Erg20p expression. The level of Erg20p was three times lower in rsp5-13 compared to the wild type strain and this effect was dependent on active Mod5p. Northern blot analysis indicated a regulatory role of Rsp5p in ERG20 transcription. ERG20 expression was also impaired in pkc1Delta lacking a component of the cell wall integrity signaling pathway. Low expression of Erg20p in rsp5 cells was accompanied by low level of ergosterol, the main end product of the isoprenoid pathway. Additionally, rsp5 strains were resistant to nystatin, which binds to ergosterol present in the plasma membrane, and sensitive to calcofluor white, a drug destabilizing cell wall integrity by binding to chitin. Furthermore, the cell wall structure appeared abnormal in most rsp5-13 cells investigated by electron microscopy and chitin level in the cell wall was increased two-fold. These results indicate that Rsp5p affects the isoprenoid pathway which has important roles in ergosterol biosynthesis, protein glycosylation and transport and in this way may influence the composition of the plasma membrane and cell wall.
Amniocentesis Following Positive First Trimester Combined Screening: A Comparative Study
Authors: Yassaee Fakhrolmolouk , Shekarriz-Foumani Reza , Sadeghi Shima
Published: December 2019

Abstract- The birth of a neonate with chromosomal abnormalities, e.g. Down syndrome has very serious problems for family, society, and for the neonate itself, and therefore prenatal evaluation is imperative in determining the fate of the fetus. This research aimed to assess the association and accuracy of amniocentesis with first-trimester combined screening. In this study, specimens from 1066 cases were analyzed for free Beta human chorionic gonadotropin, pregnancy-associated plasma protein A, along with nuchal translucency and nasal bone ultrasonography from October 2013 till November 2014. Upon observing positive screening, mothers underwent amniocentesis. Finally the amniocentesis results were compared with that of first-trimester screening. Our results determined a direct relation between the high age of the mother and gravidity with P of 0.001 and 0.020 with positive first-trimester screening. Our study attained a 92% accuracy rate of amniocentesis due to one case of mosaicism of trisomy 21, that was not diagnosed, because it was not requested by physician. Only 12 (17.1%) cases out of 70 (mothers with positive first-trimester screening) showed positive amniocentesis, which had a significant relationship with chromosomal abnormality. First trimester combined screening has very high accuracy (94.6%) in prediction of genetic abnormalities. The probability of positive first-trimester screening is directly influenced by number of factors, including the mother age and gravidity. Amniocentesis is necessary for all of mothers with positive first-trimester screening and will almost always detect chromosomal abnormalities.
A Pilot Study on Correlation Between Serum Selenium and Copper Concentrations and Coronary Slow Flow Phenomenon
Authors: Dastani Mostafa , Moshiri Soheila , Khameneh Bagheri Ramin , Bahramizadeh Maryam , Hassanzadeh Khayyat Ladan , Elyasi Sepideh , Mohammadpour Amir Hooshang
Published: December 2019

Abstract- The pathophysiology of coronary arteries slow flow phenomenon is related to the microvascular and endothelial disorder. The role of copper and selenium in the development of microvascular and endothelial disorders, as well as atherosclerosis, has been proven in previous studies. The aim of this study was to evaluate the serum copper and selenium concentrations in patient with coronary slow flow phenomenon to find a probable relationship between them. In this study, 125 patients who referred to Ghaem Hospital in Mashhad for angiography were selected based on entry and exit criteria. Patients were divided into 5 groups according to the results of angiography. Blood samples of these patients were evaluated about the levels of copper and selenium. Finally the correlation between these levels and the intensity of coronary arteries slow flow (based on TIMI scores) was evaluated. In the present study, serum selenium and copper concentrations did not show a significant correlation with the intensity of coronary arteries slow flow (P>0.05). In the present study, no significant correlation was found between copper and selenium serum concentrations and coronary arteries slow flow based on TIMI. Further studies are recommended to investigate this association.
Extensive ERG11 mutations associated with fluconazole-resistant Candida albicans isolated from HIV-infected patients
Authors: Paul Sony , Kannan Iyanar , Mohanram Kalyani
Published: December 2019

Background and Purpose: Azoles are preferred antifungal agents given their inexpensiveness, limited toxicity, and potentiality of oral administration. However, the extensive use of prophylactic azole therapy for chronic infections, especially in immunocompromised patients, has led to an increase in azole resistance, thereby rising health care costs. Fluconazole resistance is associated with poor clinical outcomes and the emergence of new infections. The present study aimed to investigate the mutations of ERG11 gene in fluconazole-resistant Candida albicans isolates.
Materials and Methods: This study was conducted on 80 clinical samples collected from HIV-infected patients with suspected candidiasis in Tagore Medical College Hospital and Government Hospital of Thoracic Medicine, Chennai, India, for a period of 18 months (May 2016-December 2017). The antifungal susceptibility pattern was determined by agar diffusion and broth dilution techniques as per the Clinical and Laboratory Standards Institute guidelines. The ERG11 gene of the known fluconazole-resistant strains of C. albicans was amplified by polymerase chain reaction (PCR). In addition, the samples were subjected to sequencing and mutation analysis.
Results: A total of 60 Candida species were isolated from HIV patients and were speciated using standard, conventional, and molecular methods. Candida albicans comprised 28.3% (n=17) of the Candida isolates, 59% (n=10) of which were resistant to fluconazole. Sequencing of the amplified product of ERG11 C. albicans gene isolates showed that they were highly mutated and included many nonsense mutations which were not reported earlier.
Conclusion: The molecular characterization of ERG11 gene showed many missense and nonsense mutations. Such mutations, which were unique to the geographical area under investigation, could be concluded to account for the development of resistance to fluconazole.
Application of platelet-rich plasma and platelet lysate as blood biomaterials in the treatment of lymphocutaneous sporotrichosis
Authors: Najafi Elahe , Anoushiravani Ali Arash , Kalafi Nooshin , Mohajerani Hamid Reza , Moradabadi Ali Reza , Mortezaeei Saman , Didehdar Mojtaba
Published: December 2019

Background and Purpose: Sporotrichosis is a subcutaneous and chronic fungal infection that is caused by a dimorphic fungus, namely Sporothrix schenckii sensu lato. Lymphocutaneous sporotrichosis is the most clinical form, which accounts for nearly 80% of the cases of cutaneous sporotrichosis. Platelets contain several substances with antimicrobial properties. Regarding this, the present study was performed to investigate the effect of blood-based biomaterials, especially platelets in the treatment of lymphocutaneous sporotrichosis.
Materials and Methods: This study was performed on 12 golden hamsters, divided into three groups of control, platelet-rich plasma, and platelet lysate. For the purpose of the study, Sporothrix conidia suspension was injected subcutaneously on the back of the animals. After the induction of subcutaneous lesions, the Gomori methenamine silver method was applied to verify lymphocutaneous sporotrichosis. Subsequently, plasma-rich platelet and platelet lysate were injected into the created lesions in the animals in 3-day intervals (due to the short lifetime of platelets). In the final sage, skin tissue samples were examined to check for the presence of yeast cells and their quantification.
Results: The data were indicative of the presence of yeast cells with/without bud in the tissue of lymphocutaneous sporotrichosis lesions in the infected animals. Histological investigation revealed that each of the two biomaterials under study (i.e., plasma-rich platelet and platelet lysate) played a positive role in the removal of the yeast cells of sporotrichosis.
Conclusion: The results of this study showed that both plasma-rich platelet and platelet lysate were able to effectively prevent from the progression of cutaneous sporotrichosis. Accordingly, much attention has been given to new therapies, including treatment with blood-derived biomaterials.
Antifungal effect of the effect of Securigera securidaca L. vaginal gel on Candida species
Authors: Raesi Vanani Atefeh , Mahdavinia Masoud , Kalantari Heibatullah , Khoshnood Saeed , Shirani Maryam
Published: December 2019

Background and Purpose: Candida species are opportunistic fungi, capable of causing acute and chronic infections in the gastrointestinal tract, vagina, and oral mucosa, among which Candida albicans is the most important species. The Securigera securidaca L. is used as an antiseptic to treat some diseases in traditional Iranian medicine. The aim of this study was to evaluate the antimicrobial activity of S. securidaca extracts and vaginal gel against different Candida species.
Materials and Methods: Antifungal effects of different extracts and vaginal gel of S. securidaca were investigated against Candida species. By using well diffusion test, different concentrations of the collected S. securidaca extracts and vaginal gel were examined to test their antifungal activity against C. albicans, C. parapsilosis, and C. krusei.
Results: The ethanol extract and vaginal gel with the ethanol extract of S. securidaca showed the most anti-fungal activity against all three strains.
Conclusion: The S. securidaca extract had a significant inhibitory effect on the different species of Candida; however, the highest inhibitory effect was found against C. albicans. In order to treat candidiasis, more research is required to check the efficacy of this plant in this domain.
High prevalence of Itraconazole Resistance among Candida parapsilosis isolated from Iran
Authors: Hassanmoghadam Fozieh , Shokohi Tahereh , Hedayati Mohammad Taghi , Aslani Narges , Haghani Iman , Nabili Mojtaba , Lotfali Ensieh , Moazeni Maryam
Published: December 2019

Background and Purpose: Candida parapsilosis isolates usually have a low minimum inhibitory concentration (MIC) against azoles. Although Candida parapsilosis isolates usually have low MICs against azoles, recent studies candida invasive infections due to azole resistant-C. parapsilosis isolates . Regarding this, the main aim of this study was to determine the susceptibility pattern of Iranian clinical C. parapsilosis against available azole antifungal drugs.
Materials and Methods: This study was conducted on 105 previously-identified isolates of C. parapsilosis sensu stricto. For the purpose of the study, the isolates were subjected to antifungal susceptibility testing against fluconazole (FLZ), itraconazole (ITZ), voriconazole (VRZ), and two new azole drugs, namely luliconazole (LUZU) and lanoconazole (LZN). The broth microdilution reference method adopted in this study was according to the Clinical & Laboratory Standards Institute M27-A3 and M27-S4 documents.
Results: According to the results, 89% (n=94) of C. parapsilosis isolates showed a MIC of ≥ 1 µg/ml, indicating resistance against ITZ. Multi-azole resistance was observed in 3.8% of the isolates. In addition, LUZU and LZN demonstrated the highest efficacy with the MIC50 values of 0.5 and 1 µg/ml, respectively.
Conclusion: The majority of the isolates showed high MIC values against ITZ. This may have been associated with the long-term ITZ prophylaxis/therapy in patients infected with candidiasis. Hence, the adoption of an appropriate antifungal agent is a crucial step for starting the treatment.
The Effect of Aqueous Extract of Tarragon on Clinical Symptoms and T Cell Differentiation in Experimental Autoimmune Encephalomyelitis
Authors: Nowras Tayebeh , Fereidouni Mohammad , Safari Hamidreza , Naseri Mohsen
Published: December 2019

Multiple sclerosis (MS) is one of the autoimmune diseases that affects the central nervous system (CNS) and causes myelin loss and axonal damage. Recent studies have shown the important role of autoreactive T cells in the pathogenesis of MS. One of the plants in the Astersa family, which has therapeutic benefits, is Artemisia dracunculus L. or Tarragon. In this study, the role of aqueous extract of Tarragon in suppressing Th1 and Th17 cell differentiation and ameliorating experimental autoimmune encephalomyelitis (EAE) was investigated.
EAE was induced in C57BL/6 female mice by Hook kit MOG35-55/CFA Emulsion PTX and one group was treated with Tarragon at a dose of 500 mg/kg. Mice were euthanized on day 33 post-immunization, spleens were removed for assessing Th1, Th17 and Treg cells by flow cytometry. We provided evidence that Tarragon (500 mg/kg) significantly ameliorated clinical scores of EAE.
We did not observe significant alterations in T cell differentiation to Th1, Th17 or Treg in the spleen of mice during EAE.
This is the first experimental evidence showing that administration of aqueous extract of Tarragon reduces the severity of EAE, but the protective effect of Tarragon is independent of alteration in T cells in the spleen. These results suggest other mechanisms for the effectiveness of this extract in improving the EAE process.
Immune Modulatory Effects of Hypercholesterolemia: Can Atorvastatin Convert the Detrimental Effect of Hypercholesterolemia on the Immune System?
Authors: Emruzi Zeinab , Babaheidarian Pegah , Arshad Mahmoud , Stockinger Hannes , Ahangari Ghasem
Published: December 2019

Many observations showed that hypercholesterolemia can disrupt immune response. Statin drugs that were used for the treatment of hypercholesterolemia patients can interfere in the regulation of the immune response and cytokine secretion. The primary aim of the current study was to investigate the immune response among treatment-naïve patients with hypercholesterolemia and healthy subjects. The secondary goal of the study was to determine whether atorvastatin can reverse the detrimental effect of hypercholesterolemia on the immune system.
Peripheral blood mononuclear cells (PBMCs) were isolated from 50 patients afflicted with hypercholesterolemia who were treatment-naïve along with 50 sex/age-matched hypercholesterolemia patients receiving atorvastatin, and 50 sex/age-matched healthy subjects. Quantitative PCR and ELISA methods were used for gene and protein expression analysis of T helper 1 (Th1) and Th2 related cytokines. Additionally, the expression of the cluster of differentiation (CD) markers on T, B, and natural killer (NK) cells was measured by flow cytometry method.
The results showed that hypercholesterolemia and atorvastatin down-regulated the expression of Th1-related cytokines and elevated the levels of Th2-related cytokines. The expression of cell surface markers, CD25 and CD69, was significantly decreased in the treatment-naïve, and atorvastatin groups.
It seems that atorvastatin is not able to repair the deleterious effects of hypercholesterolemia on the immune system. Moreover, elevated levels of cholesterol along with the administration of atorvastatin tilt the Th1/Th2 balance in favor of Th2 and reduce T cell activation.
Dihydrotestosterone and 17-Estradiol Enhancement of In Vitro Osteogenic Differentiation of Castrated Male Rat Bone Marrow Mesenchymal Stem Cells (rBMMSCs)
Authors: Abo-Aziza FAM , Zaki AA , Amer AS , Lotfy RA
Published: December 2019

Background: In vitro impact of dihydrotestosterone (DHT) and 17-estradiol (E2) in osteogenic differentiation of castrated rat bone marrow mesenchymal stem cells (rBMMSC) still need to be clarified. Materials and Methods: The viability, proliferation and density of cultured rBMMSC isolated from sham operated (Sham) and castrated (Cast) male rats were evaluated. rBMMSC were cultured with osteogenic differentiating medium (ODM) in the presence of DHT (5,10 nM) and E2 (10,100 nM). Osteogenesis was evaluated by alizarin red staining and measurement of calcium deposition and bone alkaline phosphatase (BALP) activity. Results: Population doubling (PD) of rBMMSC isolated from Cast rats was significantly lower (P<0.05) compared to that isolated from Sham rats. rBMMSC from Cast rats showed low scattered calcified nodule after culturing in ODM and did not cause a significant increase in calcium deposition and B-ALP activity compared to rBMMSCs from Sham rats. Exposure of rBMMSC isolated from Cast rats to DHT (5 nM) or E2 (10 nM) in ODM showed medium scattered calcified nodules with significantly higher (P<0.05) calcium deposition and B-ALP activity. Moreover, exposure of rBMMSC to DHT (10 nM) or E2 (100 nM) showed high scattered calcified nodules with higher (P<0.01) calcium deposition and B-ALP activity Conclusion: These results indicated that the presence of testes might participate in controlling the in vitro proliferation and osteogenic differentiation capacity of rBMMSCs. DHT and E2 can enhance the osteogenic capacity of rBMMSCs in a dose-dependent manner. Based on these observations, optimum usage of DHT and E2 can overcome the limitations of MSCs and advance the therapeutic bone regeneration potential in the future.
The Relationship between Self-esteem and Anxiety with Severity of Pain and Suffering of Labor
Authors: Baghani Roya , Sharifzadeh Masoumeh , Navi Nezhad Mozhdeh , Keramat Afsaneh , Khosravi Ahmad
Published: December 2019

Introduction: Recognition of labor pain is a primary concern of physicians, midwives and their clients in controlling labor pain and preventing the resulting suffering. The purpose of this study was to assess the relationship between self-esteem and anxiety with severity of pain and suffering of labor in women. Method: This cross-sectional study was conducted on 365 mothers who experienced delivery at Shahidan Mobini hospital in Sabzevar in 2014. The data were collected using Rosenberg Self-esteem questionnaire and Spielberger State-Trait Anxiety scales, visual analogue scale (VAS) for pain, and experience and perception of suffering. Data were analyzed using t-test, Pearson correlation coefficient and chi-square test considering level of significance equal to 0.05. Results: The mean age was 26.4± 4.7 years old. In this study, 119 (44.9%) samples were nulliparous, 20 (7.5%) were employed and 196 (74%) were urban citizens. There was no significant difference between the pain of labor score in terms of self-esteem levels (p=0.84) and the level of covert anxiety (p=0.15) and overt anxiety (p=0.06). However, there was a significant differences between the suffering of labor scores in terms of selfesteem levels (p<0.001),the covert anxiety levels (p<0.001) and the overt anxiety levels (p<0.05). Conclusion: Considering pregnant women with higher self- esteem and lower anxiety score had a lower score on labor suffering in this study, it seems health care providers can help reduce pregnant women’s suffering of labor through strengthening the well-being and health of pregnant women and supporting them during pregnancy and labor.
Efficacy of Mindfulness Based on Cognitive Therapy on Impulsivity and Self- Inhibition in Patients with Bipolar Disorder under Medical Treatment
Authors: Kazemi-Zahrani Hamid , Mohagheghian Malihe
Published: December 2019

Introduction: Bipolar disorder is combined with chronic symptoms that have effect on cognitive abilities and executive functions. The purpose of present study was to investigate efficacy of mindfulness based cognitive therapy (MBCT) on impulsivity and self-inhibition in the patients with bipolar disorder under medical treatment.
Methods: This study was quasi-experimental, pretest-posttest design with the control group. 30 men and women with bipolar disorder referred to psychiatry center of Almahdi Welfare in Esfahan were selected through convenience sampling method and assigned in two groups: experimental (14) and control (16) randomly. Both groups performed behavioral impulsivity scale (BIS-11) and self-inhibition scale (SRS) in the pretest and posttest phases. Experimental group was treated with mindfulness based cognitive therapy (MBCT) for 8 sessions. The data were analyzed by MANCOVA.
Results: Covariate analyze demonstrated that  of mindfulness based cognitive therapy (MBCT) was effective on reducing the impulsivity and increasing the self-inhibition in the experimental group.
Conclusion: Based on this finding, reduce cognitive impulsivity and motor impulsivity, and increase self-inhibition in anger control, impulse control and respect for others dimensions, is because of mindfulness based on cognitive therapy. Considering psychological intervention based on mindfulness, in addition to medical treatment is of particular importance.
PEDAGOGICAL REQUIREMENTS FOR THE IMPLEMENTATION OF DIFFERENTIATED APPROACH IN TEACHING ELEMENTARY SCHOOL CHILDREN
Authors: Faridovna Dulmukhametova Gulnara , Nailevna Sirazieva Zarina , Faritovna Gali Gulnara , Svetlana Nicolaevna Usova , Valievna Shamsutdinova Dilbar
Published: December 2019

Purpose of the Study: The article describes the view of the authors on the model of the implementation of a differentiated approach in the training of elementary school children. Methodology: In the research the authors identify and systematize the pedagogical requirements for ensuring the sex differentiation in the education of elementary school children, together forming a meaningful model for implementing a gender approach to the educational process of primary school and including gender education for students, teachers, and parents. The authors analyze the category of sex differentiation in domestic and foreign pedagogy. Result: The authors theoretically describe the problem of implementing a differentiated approach in teaching elementary school children, and also substantiate the experimental work on testing the effectiveness of pedagogical conditions that interconnect and complement each other, forming a dynamic system that ensures the effective organization of the sex differentiation of elementary school children. Application and Novelty: Presented pedagogical requirements and model of a gender approach to the educational process can solve the age and gender problems of elementary school children and harmonize their relationships. Data of the article can be used not only in the educational process of elementary school but also at the lessons of such subjects as "Pedagogical psychology", "Developmental psychology", "Differential psychology" at the university. These requirements give the opportunity to prepare more qualified and competent psychologists and teachers for elementary school.
La modalidad de atención a niños y niñas hasta los tres (03) años de edad en la reclusión de mujeres de Bogotá: análisis y recomendaciones de política
Authors: Isaza De Francisco Olga , Camacho Sandra , García María Camila , Jaramillo Andrea , Salinas Olga
Published: December 2019

El objetivo de la investigación es realizar un análisis de política y formular recomendaciones que contribuyan a que el Modelo de Atención a niños y niñas hasta los tres (03) años de edad, en la Reclusión de Mujeres de Bogotá, cumpla con los criterios básicos para la garantía de sus derechos. Para tal efecto, se utilizó una metodología de análisis de políticas públicas, de corte cualitativo. La investigación permitió identificar que la modalidad de atención, tal y como se encuentra estructurada, no cumple con los criterios básicos para garantizar los derechos de los niños y niñas que permanecen al interior de la reclusión de mujeres de Bogotá, por lo cual se recomienda que se lleve a cabo el rediseño de la modalidad.
Ketogenic Diet for Refractory Childhood Epilepsy: Beyond Seizures Control, the Experience of a Portuguese Pediatric Centre
Authors: Romão Luz Inês , Pereira Cristina , Garcia Paula , Ferreira Fátima , Faria Ana , Macedo Cristiane , Diogo Luísa , Robalo Conceição
Published: December 2019

Introduction: Ketogenic diet is a low carbohydrate diet, which can be used as a treatment for refractory childhood epilepsy. The first aim of this study was to evaluate its efficacy, in patients receiving ketogenic diet for at least three months, on epilepsy control, behaviour and awareness. The secondary aims were to evaluate the variation in the number of antiepileptic drugs, reasons for discontinuing the diet and adverse effects.Material and Methods: Retrospective analysis of clinical records of patients who underwent ketogenic diet for refractory epilepsy, from October 2007 to January 2018, in a tertiary pediatric hospital.Results: In the twenty-nine eligible patients, the mean age of initiation was 7.9 years-old (+/– 5.6). Of those, 18 had a ≥ 50% reduction of seizure activity, 19 a marked behaviour improvement and 18 improved awareness. The median number of antiepileptic drugs remained equal for the 15 patients who completed 18 months of treatment (three drugs). The main reason for discontinuing ketogenic diet was a familiar decision. The main adverse effects were hypercholesterolemia (n = 23) and hypertriglyceridemia (n = 21).Discussion: Results were comparable to those of other cohorts, namely age of initiation, proportion of patients completing ketogenic diet, most frequent reasons for stopping and significant improvement of alertness and behavior.Conclusion: Beyond seizure control, patients experienced a marked improvement in behavior and awareness. It is necessary to develop strategies to increase the adherence of families to the diet.
Errata a “A Cirurgia de Substituição Valvular Aórtica Melhora a Qualidade de Vida dos Doentes?” Pedro COELHO, Lara N. FERREIRA, Clara VITAL, José FRAGATA. Acta Med Port 2018 Jul-Aug;31(7-8):399-408
Authors: Coelho Pedro , Ferreira Lara N. , Vital Clara , Fragata José
Published: December 2019

Artigo publicado com erros: https://actamedicaportuguesa.com/revista/index.php/amp/article/view/10241Na página 405, Tabela 3 onde se lê:A negrito o valor 34,4 na dimensão "limitação do Desempenho" referente à coluna "Seguimento a 6 meses"Deverá ler-se:A negrito o valor 37,9. Na página 406, Figura 4, onde se lê:O eixo do índice SF-6D com valores entre 0 a 50.Deverá ler-se:O eixo do índice SF-6D com valores entre 0 a 1.
Passive Transfer of Hepatitis B Antibodies through Intravenous Immunoglobulin in a Neonate
Authors: Rato João , Alves Daniela , Rodrigues Luís
Published: December 2019

Passive transfer of antibodies secondary to intravenous immunoglobulin infusion is a rare but important side effect that can lead to the wrong diagnosis and therapeutic decisions. It has never been reported in a newborn. A male newborn, vaccinated against hepatitis B and diagnosed with dilated cardiomyopathy, presented positive hepatitis B core antibodies at 12 days of life. Exclusion of hepatitis B infection was mandatory as it would be a contraindication to heart transplant. Passive transfer of antibodies was confirmed at 44 days of age, after seroreversion of hepatitis B core antibodies. Passive transfer of antibodies after intravenous immunoglobulin infusion can lead to a misleading diagnosis if not recognized. In our patient it could have been especially harmful had it prevented heart transplant. Screening for hepatitis B should be performed at least 1 month after intravenous immunoglobulin infusion.
Formulasi Tablet Paracetamol dengan Bahan Pengikat Pati Umbi Gembili (Dioscorea esculenta L)
Authors: Zulfa Elya , Prihantini Malinda
Published: December 2019

ABSTRAK Pati umbi gembili (PUG) memiliki kandungan utama amilopektin sebesar 75,7%. Amilopektin bersifat lekat dan dapat membentuk gel bila disuspensikan dengan air, sehingga dapat dimanfaatkan sebagai bahan pengikat. Tujuan penelitian ini adalah mengetahui sifat fisik tablet paracetamol dengan penambahan PUG sebagai bahan pengikat.  Proses pembuatan pati dari umbi gembili dilakukan dengan cara basah. Tablet Parasetamol dibuat dengan metode granulasi basah dengan variasi kadar mucilago PUG FI 5%, FII 10%, FIII 15%. Tablet yang dibuat, diuji sifat fisik (keseragaman bobot, kekerasan, kerapuhan dan waktu hancur) dan dianalisis secara deskriptif. Hasil uji sifat fisik tablet menunjukkan bahwa seluruh formula yang dibuat memenuhi syarat yang ditetapkan dalam kompendial. Kata Kunci :  Parasetamol, pati umbi gembili, bahan pengikat, granulasi basah  ABSTRACT Gembili tuber (PUG) starch has a major component of amylopectin of 75.7%. Amylopectin is sticky and can form a gel when suspended with water, so that it can be used as a tablet binding agent in the form of PUG mucilago. The purpose of this study was to determine the physical characteristics in paracetamol tablets of adding PUG mucilago (Dioscorea esculenta) as a binder. The process of making starch from gembili tuber is done by wet. Paracetamol tablets are made by wet granulation method with variations in levels of FI PUG mucilago 5%, FII 10%, FIII15%. Tablets were made, tested for physical properties (weight uniformity, hardness, friability and disintegration time) with descriptive analysis. The test results of the physical properties of tablets indicate that all formulas made meet the requirements set out in the literature Keywords: Paracetamol, gembili tuber starch, binder, wet granulation
Efek Sitotoksik Ekstrak Etanolik Ocimum basilicum, L. Pada Sel Kanker Payudara
Authors: Hidayati Devi Nisa , Arifin Ibrahim , Zahroh Fatimatuz , Wahyuni Lina
Published: December 2019

ABSTRAK Pengobatan kanker menggunakan bahan alam terus dikembangkan. Salah satu tanaman yang memiliki efek sitotoksik Ocimum basilicum, L. tujuan penelitian ini adalah mengetahui aktivitas sitotoksik dari ekstrak etanol Ocimum basilicum (EEOB) terhadap sel kanker payudara T47D dan MCF7. Ocimum basilicum diekstraksi menggunakan metode maserasi dengan pelarut etanol 70%. Pengujian aktivitas sitotoksik menggunakan metode MTT assay dengan  seri konsentrasi EEOB 1000; 500; 250; 125; 62.5; 31,25 µg/mL. Hasil uji aktivitas sitotoksik EEOB memperlihatkan nilai IC50 pada sel T47D dan MCF-7 sebesar 399.86 µg/ml dan 387.76 µg/ml. Kata Kunci—Sitotoksik, Ocinum basilicum L., T47D, MCF-7  ABSTRACT             Cancer treatment using natural ingredients continues to be developed. One of the plants that is proven to have cytotoxic activity is basil leaves (Ocimum basilicum, L.). This study aims to determine the cytotoxic activity of ethanol extract of basil leaves (EEBL) on T47D and MCF-7 breast cancer cells. Basil leaves were extracted using maceration  with ethanol 70%. The cytotoxic test was perfomed using MTT assay with various EEBL concentrations: 1000; 500; 250; 125; 62.5; 31,25 µg/mL. The results showed that IC50 of cytotoxic activity in T47D and MCF-7 was 399.86 µg/ml and 387.76 µg/ml respectively. Keywords—Cytotoxic, Ocinum basilicum L., T47D, MCF-7
Gambaran Tingkat Pengetahuan Masyarakat Tentang Obat Generik di Kelurahan Keraton Kecamatan Martapura Kabupaten Banjar
Authors: Fitriah Rahmayanti , Mahriani Mahriani , Nurrahma Ika Maulida
Published: December 2019

ABSTRAK Obat generik adalah obat yang penamaannya didasarkan pada kandungan zat aktif tertentu dalam suatu obat dan tidak menggunakan merk dagang. Persepsi pasien terhadap obat generik di masa penerapan JKN ini dinilai oleh banyak pengamat masih buruk, salah satunya yang menyatakan bahwa masih ada persepsi yang salah tentang obat generik, yaitu obat generik dianggap sebagai obat murah sehingga mutunya diragukan. Penelitian ini bertujuan untuk mengetahui gambaran tingkat pengetahuan masyarakat terhadap obat generik di Kelurahan Keraton Kecamatan Martapura Kabupaten Banjar Kalimantan Selatan Indonesia. Penelitian ini merupakan penelitian deskriptif menggunakan desain cross sectional. Pengambilan data dilakukan melalui lembar kuesioner, dengan sampel yang dipilih menggunakan metode Stratified Random Sampling. Berdasarkan hasil penelitian yang dilakukan melalui uji kuesioner kepada 100 responden untuk menilai gambaran tingkat pengetahuan masyarakat tentang obat generik. Dari total sampel dijumpai bahwa 45 orang (45%) responden memiliki tingkat pengetahuan dengan katagori “sedang”. Kepada para pembuat kebijakan kesehatan diharapkan terus meningkatkan sosialisasi obat generik. Kepada pelayanan kesehatan, agar dapat meningkatkan kinerja dalam penyuluhan program promosi obat generik di daerah. Kata Kunci :  Obat Generik, Masyarakat, Tingkat Pengetahuanl  ABSTRACT             Generic drugs are drugs that are named after the activecontained, and not using a trademark. The community’s perception of generic drugs during the JKN application was deemed unsavory by many observers, generic drugs are considered as cheap medicine with doubtable quality. This study aims to determine the level of public knowledge about generic drugs in Kelurahan Keraton Kecamatan Martapura Kabupaten Banjar South Borneo Indonesia. This research is a descriptive researchusing a cross sectional design. The data were collected through a questionnaire, with the sampled selection using Stratified Random Sampling method. Based on the results of the research conducted by the questioning 100 respondents to obtain the level of public knowledge about generic drugs. Of the total sample found, (45%) respondents (45%) has a knowledge level with the category "medium". To health policy makers to continue to increase the socialization of generic drugs. To health services, to improve education programs in the promotion of generic programs in the region. Keywords : Generic Drugs, Society, Level of Knowledge
Análises das experiências de educação patrimonial no ensino de história pelos estudantes das escolas EREM Porto Digital e EREM Paulista (Pernambuco/Brasil)
Authors: Nathália Nunes Tristão Isabela , Silva Adriana Maria Paulo da
Published: December 2019

Resultado de um Trabalho de conclusão de curso, esta pesquisa emergiu de um conjunto de atividades vinculadas aos projetos “Retratos do Recife: século XX, memória e patrimônio” e “Tecelões da memória operária: a História do Paulista didatizada” e desenvolvidas por bolsistas do Programa Institucional e Bolsa de Iniciação à Docência (PIBID-História) da Universidade Federal de Pernambuco, nos anos de 2014 e 2015, em duas escolas estaduais de referência do ensino médio: EREM Porto Digital (localizada no Recife Antigo/ PE) e EREM Paulista (no bairro da Torres Galvão/ PE), respectivamente, cujo objetivo foi vivenciar a Educação Patrimonial através do estudo da História Local. Dos resultados dos referidos projetos, aprofundamos a temática em questão, por intermédio de entrevistas feitas a grupos de estudantes das duas ditas escolas– metodologia de grupo focal– com o objetivo de analisar as experiências de Educação Patrimonial na vida dos entrevistados. Percebemos que, de maneira geral, as atividades com Educação Patrimonial geraram um sentimento de pertencimento e uma ampliação de conhecimentos históricos, sociais e culturais sobre os bairros estudados.
Finishing of Nickel Matrix Composite Coatings
Authors: Dyl Tomasz , Starosta Robert
Published: December 2019

Metal matrix composite are used in such fields of technology, such as: aerospace, electronics, energy, industry, defense, automotive, aviation, shipbuilding, and more. Composite coatings of ceramic - metals is used primarily to enhance the durability of machine parts. Therefore, new materials are permanently looked for, what has resulted in the past in development of composite materials. The coatings dispersed are consisting of metallic matrix (metals and their alloys) and small non-metallic particles. The deposition of ceramic particles simultaneously with metallic matrix leads often to composite coatings possessing properties much better than those of metallic coating. The nickel and less often, other iron group elements are usually used as a matrix and Al2O3 as tough particles. The welding technology of applying alloy and composite coatings is widely used. The technology of infrasound thermal spraying of metal matrix composite coatings was presented. It is a simple technology and a very useful one in ship machinery regeneration during the cruise craft (e.g. internal combustion engines, torque pumps, separators). The metal matrix composite coatings must undergo finishing due to high surface roughness after application. In the article to used finishing by plastic working and machining of coatings nickel matrix composite was proposed.
Development of a 3D-Printable Ultra-High Performance Fiber-Reinforced Concrete for Digital Construction
Authors: Arunothayan Ravendran , Nematollahi Behzad , Ranade Ravi , Bong Shin Hau , Sanjayan Jay
Published: December 2019

This paper presents the systematic development and performance characterization of a non-proprietary 3D-printable ultra-high-performance fiber-reinforced concrete (UHPFRC) for digital construction. Several fresh and hardened properties of the developed 3D-printable UHPFRC matrix (without fiber) and composite (with 2% volume fraction of steel fibers) were evaluated and compared to that of conventionally mold-cast UHPFRC. Additionally, the effects of testing direction on the compressive and flexural strengths of the printed UHPFRC were investigated. The fresh properties of the UHPFRC developed in this study satisfied the criteria for extrudability, buildability, and shape-retention-ability, which are relevant for ensuring printability. The printed UHPFRC exhibited superior flexural performance to the mold-cast UHPFRC due to alignment of the short fibers in the printing direction. The high compressive and flexural strengths, along with the deflection-hardening behavior, of the developed UHPFRC can enable the production of thin 3D-printed components with significant reduction or complete elimination of conventional steel bars.
Teamwork for Multi-Robot Systems in Dynamic Environments
Authors: Geihs Kurt
Published: December 2019

The increasing number of robots around us will soon create a demand for connecting these robots in order to achieve goal-driven teamwork in heterogeneous multi-robot systems. In this paper, we focus on robot teamwork specifically in dynamic environments. While the conceptual modeling of multi-agent teamwork has been studied extensively during the last two decades, related engineering concerns have not received the same degree of attention. Therefore, this paper makes two contributions. The analysis part discusses general design challenges that apply to robot teamwork in dynamic application domains. The constructive part presents a review of existing engineering approaches for challenges that arise with dynamically changing runtime conditions. An exhaustive survey of robot teamwork aspects would be beyond the scope of this paper. Instead, we aim at creating awareness for the manifold dimensions of the design space and highlight state-of-the-art technical solutions for dynamically adaptive teamwork, thus pointing at open research questions that need to be tackled in future work.
Why Should Growth Hormone (GH) Be Considered a Promising Therapeutic Agent for Arteriogenesis? Insights from the GHAS Trial
Authors: Caicedo Diego , Devesa Pablo , Alvarez Clara , Devesa Jesús
Published: December 2019

Despite the important role that the GH/IGF-I axis plays in vascular homeostasis, these kind of growth factors barely appear in articles addressing the neovascularization process. Currently, the vascular endothelium has turned to be considered as an authentic gland of internal secretion due to the wide variety of released factors and functions with local effect, including the paracrine/autocrine production of GH or IGF-I, for which the endothelium has specific receptors. In this comprehensive review, it will be described the evidence involving these proangiogenic hormones in arteriogenesis dealing with the arterial occlusion and making of them a potential therapy. It will be analyzed all those elements triggering the local and systemic production of GH/IGF-I and their possible role both in physiological and pathological conditions. The whole evidence will be combined with important data from the GHAS trial, in which GH or placebo were administrated to patients suffering from critical limb ischemia with no option for revascularization. We postulate that GH, alone or in combination, should be considered as a promising therapeutic agent for helping in the approach of the ischemic disease.
Validation of a Quantitative Proton Nuclear Magnetic Resonance Spectroscopic Screening Method for Coffee Quality and Authenticity (NMR Coffee Screener)
Authors: Okaru Alex O. , Scharinger Andreas , Rajcic de Rezende Tabata , Teipel Jan , Kuballa Thomas , Walch Stephan G. , Lachenmeier Dirk W.
Published: December 2019

Monitoring coffee quality as a means of detecting and preventing economically motivated fraud is an important aspect of international commerce today. Therefore, there is a compelling need for rapid high throughput validated analytical techniques such as quantitative proton NMR spectroscopy for screening and authenticity testing. For this reason, we sought to validate an NMR spectroscopic method for routine screening of coffee for quality and authenticity. A factorial experimental design was used to investigate the influence of NMR device, extraction time and nature of coffee on the content of caffeine, 16-O-methylcafestol (OMC), kahweol, furfuryl alcohol and 5-hydroxymethylfurfural (HMF) in coffee. The method was successfully validated for specificity, selectivity, sensitivity and linearity of detector response. The proposed method produced satisfactory precision for all analytes in roasted coffee, except for kahweol in canephora (robusta) coffee. The proposed validated method may be used for routine screening of roasted coffee for quality and authenticity control, as its applicability was demonstrated during the recent OPSON VIII Europol-Interpol operation on coffee fraud control.