bluuebunny/crossref_metadata_2025 huggingface dataset:
Cyclic Plasticity and Low Cycle Fatigue of an AISI 316L Stainless Steel: Experimental Evaluation of Material Parameters for Durability Design
Authors: Pelegatti Marco , Lanzutti Alex , Salvati Enrico , Srnec Novak Jelena , De Bona Francesco , Benasciutti Denis
Published: June 2021

AISI 316L stainless steels are widely employed in applications where durability is crucial. For this reason, an accurate prediction of its behaviour is of paramount importance. In this work, the spotlight is on the cyclic response and low-cycle fatigue performance of this material, at room temperature. Particularly, the first aim of this work is to experimentally test this material and use the results as input to calibrate the parameters involved in a kinematic and isotropic nonlinear plasticity model (Chaboche and Voce). This procedure is conducted through a newly developed calibration procedure to minimise the parameter estimates errors. Experimental data are eventually used also to estimate the strain–life curve, namely the Manson–Coffin curve representing the 50% failure probability and, afterwards, the design strain–life curves (at 5% failure probability) obtained by four statistical methods (i.e., deterministic, “Equivalent Prediction Interval”, univariate tolerance interval, Owen’s tolerance interval for regression). Besides the characterisation of the AISI 316L stainless steel, the statistical methodology presented in this work appears to be an efficient tool for engineers dealing with durability problems as it allows one to select fatigue strength curves at various failure probabilities depending on the sought safety level.
Raman and X-ray Photoelectron Spectroscopic Study of Aqueous Thiol-Capped Ag-Zn-Sn-S Nanocrystals
Authors: Dzhagan Volodymyr , Selyshchev Oleksandr , Havryliuk Yevhenii , Mazur Nazar , Raievska Oleksandra , Stroyuk Oleksandr , Kondratenko Serhiy , Litvinchuk Alexander P. , Valakh Mykhailo Ya. , Zahn Dietrich R. T.
Published: June 2021

The synthesis of (Cu,Ag)-Zn-Sn-S (CAZTS) and Ag-Zn-Sn-S (AZTS) nanocrystals (NCs) by means of “green” chemistry in aqueous solution and their detailed characterization by Raman spectroscopy and several complementary techniques are reported. Through a systematic variation of the nominal composition and quantification of the constituent elements in CAZTS and AZTS NCs by X-ray photoemission spectroscopy (XPS), we identified the vibrational Raman and IR fingerprints of both the main AZTS phase and secondary phases of Ag-Zn-S and Ag-Sn-S compounds. The formation of the secondary phases of Ag-S and Ag-Zn-S cannot be avoided entirely for this type of synthesis. The Ag-Zn-S phase, having its bandgap in near infrared range, is the reason for the non-monotonous dependence of the absorption edge of CAZTS NCs on the Ag content, with a trend to redshift even below the bandgaps of bulk AZTS and CZTS. The work function, electron affinity, and ionization potential of the AZTS NCs are derived using photoelectron spectroscopy measurements.
Polyisobutylene and Silicone in Warm Edge Glazing Systems—Evaluation of Long-Term Performance
Authors: Cwyl Maciej , Michalczyk Rafał , Wierzbicki Stanisław
Published: June 2021

This article describes the characteristics of one type of sealing system used in warm edge glazing units and analyses the possible causes of damage. Attention was focused on the performance of the dual seal, PIB/silicone system. This type of glazing is widely used for modern curtain walls and roofs of office buildings and shopping centres. Study was focused on PIB displacement defects, which affects both the appearance and thermal performance of the curtain wall system. Wide-ranging field surveys were conducted to examine the problems identified in some office buildings. The information gathered in this way was used to identify the critical areas and causes of seal displacement in the analysed insulating glass units (IGUs). Laboratory tests were conducted on PIB and silicone seals retrieved from the removed defective units. The properties of these materials were determined and used to evaluate the applied edge sealing system and build a representative numerical model. Due to the problems encountered in deriving accurate analytical formulas, finite element (FE) approximation was used as a problem solving tool. The generated FE model and strain analysis were the key parts to obtaining a true representation of the actual behaviour of IGUs subjected to various environmental loads, taking into account the influence of the air cavity. Results of computer simulations and laboratory tests were compared for model validation. The effect of changes in ambient pressure was examined, showing the development of tensile strains in the silicone and PIB, which can lead to debonding. The greatest principal strains occur at the silicone/butyl rubber interface and this location should be considered to be the most susceptible to failure. The observations are summarised in the final conclusions. Additionally, as field study showed, after ten years in service, the percentage of damaged units is considerable. More frequent IGUs inspection should cover both appearance and thermal imaging to detect unsealed panels. From the standpoint of both durability and appearance, dual silicone/PIB should be phased out in favour of modern seal systems.
Analysis of Post-Deposition Recrystallization Processing via Indium Bromide of Cu(In,Ga)Se2 Thin Films
Authors: Poudel Deewakar , Belfore Benjamin , Ashrafee Tasnuva , Karki Shankar , Rajan Grace , Rockett Angus , Marsillac Sylvain
Published: June 2021

Cu(In,Ga)Se2 (CIGS) thin films were deposited at low temperature (350 °C) and high rate (10 µm/h) by a single stage process. The effect of post-deposition treatments at 400 °C and 500 °C by indium bromide vapor were studied and compared to the effect of a simple annealing under selenium. Structural, electrical, and chemical analyses demonstrate that there is a drastic difference between the different types of annealing, with the ones under indium bromide leading to much larger grains and higher conductivity. These properties are associated with a modification of the elemental profiles, specifically for gallium and sodium.
Development and Performance Evaluation of a High-Permeability and High-Bonding Fog-Sealing Adhesive Material
Authors: Tian Tian , Jiang Yingjun , Fan Jiangtao , Yi Yong , Deng Changqing
Published: June 2021

Herein, the effects of the contents of emulsified asphalt, waterborne epoxy resin emulsion, and curing agent on the permeability, bond shear strength, water stability, and aging resistance of epoxy-emulsified asphalt were studied. A formulation of epoxy-emulsified asphalt as a fog-sealing adhesive material was recommended, and a comparison between the fabricated adhesive material and a traditional Chinese fog-sealing adhesive material was conducted to verify the technical performance of the new material. In addition, the strength formation mechanism of the epoxy-emulsified asphalt was revealed via microcosmic analysis. Results show that the curing agent content mainly affects the permeability of epoxy-emulsified asphalt, and the emulsified asphalt content significantly affects the bond shear strength, water stability, and aging resistance. Moreover, the ratio of waterborne epoxy resin emulsion to the curing agent (epoxy ratio) has a certain effect on the bond shear strength. In the recommended formulation (a high-permeability and high-bonding fog-sealing adhesive material, which can be referred to simply as HPBFA), emulsified asphalt accounts for 80% of the total mass of the mixture, and the epoxy ratio is 2:1–3:1. It can improve air permeability, bond shear strength, water stability and aging resistance. The HPBFA-cured material exhibits a continuous three-dimensional network structure, hydrophobic surface, and large contact angle. Furthermore, the initial thermal weight loss temperature of the HPBFA-cured material is significantly higher than the environmental aging temperature. Additionally, the maximum temperature decomposition range is 0–160 °C, indicating improved strength, wear resistance, permeability, and aging resistance of the material.
Directionally-Dependent Mechanical Properties of Ti6Al4V Manufactured by Electron Beam Melting (EBM) and Selective Laser Melting (SLM)
Authors: Pasang Tim , Tavlovich Benny , Yannay Omri , Jackson Ben , Fry Mike , Tao Yuan , Turangi Celine , Wang Jia-Chang , Jiang Cho-Pei , Sato Yuji , Tsukamoto Masahiro , Misiolek Wojciech Z.
Published: June 2021

An investigation of mechanical properties of Ti6Al4V produced by additive manufacturing (AM) in the as-printed condition have been conducted and compared with wrought alloys. The AM samples were built by Selective Laser Melting (SLM) and Electron Beam Melting (EBM) in 0°, 45° and 90°—relative to horizontal direction. Similarly, the wrought samples were also cut and tested in the same directions relative to the plate rolling direction. The microstructures of the samples were significantly different on all samples. α′ martensite was observed on the SLM, acicular α on EBM and combination of both on the wrought alloy. EBM samples had higher surface roughness (Ra) compared with both SLM and wrought alloy. SLM samples were comparatively harder than wrought alloy and EBM. Tensile strength of the wrought alloy was higher in all directions except for 45°, where SLM samples showed higher strength than both EBM and wrought alloy on that direction. The ductility of the wrought alloy was consistently higher than both SLM and EBM indicated by clear necking feature on the wrought alloy samples. Dimples were observed on all fracture surfaces.
Energy Storage Ceramics: A Bibliometric Review of Literature
Authors: Hu Haiyan , Liu Aiping , Wan Yuehua , Jing Yuan
Published: June 2021

Energy storage ceramics is among the most discussed topics in the field of energy research. A bibliometric analysis was carried out to evaluate energy storage ceramic publications between 2000 and 2020, based on the Web of Science (WOS) databases. This paper presents a detailed overview of energy storage ceramics research from aspects of document types, paper citations, h-indices, publish time, publications, institutions, countries/regions, research areas, highly cited papers, and keywords. A total of 3177 publications were identified after retrieval in WOS. The results show that China takes the leading position in this research field, followed by the USA and India. Xi An Jiao Tong Univ has the most publications, with the highest h-index. J.W. Zhai is the most productive author in energy storage ceramics research. Ceramics International, Journal of Materials Science-Materials in Electronics, and the Journal of Alloys and Compounds are the most productive journals in this field, and materials science—multidisciplinary is the most frequently used subject category. Keywords, highly cited papers, and the analysis of popular papers indicate that, in recent years, lead-free ceramics are prevalent, and researchers focus on fields such as the microstructure, thin films, and phase transition of ceramics.
Effect of Initial Temperature on the Microstructure and Properties of Stellite-6/Inconel 718 Functional Gradient Materials Formed by Laser Metal Deposition
Authors: Yao Jun , Xin Bo , Gong Yadong , Cheng Guang
Published: June 2021

Stelite-6/Inconel 718 functionally gradient materials (FGM) is a heat-resisting functional gradient material with excellent strength performance under ultra-high temperatures (650–1100 °C) and, thus, has potential application in aeronautic and aerospace engineering such as engine turbine blade. To investigate the effect of initial temperature on the microstructure and properties of laser metal deposition (LMD) functional gradient material (FGM), this paper uses the LMD technique to form Stelite-6/Inconel 718 FGM at two different initial temperatures: room temperature and preheating (300 °C). Analysis of the internal residual stress distribution, elemental distribution, microstructure, tensile properties, and microhardness of 100% Stelite-6 to 100% Inconel 718 FGM formed at different initial temperatures in a 10% gradient. The experimental results prove that the high initial temperature effectively improves the uneven distribution of internal residual stresses. Preheating slows down the solidification time of the melt pool and facilitates the escape of gases and the homogeneous diffusion of elements in the melt pool. In addition, preheating reduces the bonding area between the gradient layers, enhancing the metallurgical bonding properties between the layers and improving the tensile properties. Compared with Stellite-6/Inconel 718 FGM formed at room temperature, the mean yield strength, mean tensile strength, and mean elongation of Stellite-6/Inconel 718 FGM formed at 300 °C are increased by 65.1 Mpa, 97 MPa, and 5.2%. However, the high initial temperature will affect the hardness of the material. The average hardness of Stellite-6/Inconel 718 FGM formed at 300 °C is 26.9 HV (Vickers hardness) lower than that of Stellite-6/Inconel 718 FGM formed at 20 °C.
Effects of Plasma Treated Alumina Nanoparticles on Breakdown Strength, Partial Discharge Resistance, and Thermophysical Properties of Mineral Oil-Based Nanofluids
Authors: Saman Norhafezaidi Mat , Zakaria Izzah Hazirah , Ahmad Mohd Hafizi , Abdul-Malek Zulkurnain
Published: June 2021

Mineral oil has been chosen as an insulating liquid in power transformers due to its superior characteristics, such as being an effective insulation medium and a great cooling agent. Meanwhile, the performance of mineral oil as an insulation liquid can be further enhanced by dispersing nanoparticles into the mineral oil, and this composition is called nanofluids. However, the incorporation of nanoparticles into the mineral oil conventionally causes the nanoparticles to agglomerate and settle as sediment in the base fluid, thereby limiting the improvement of the insulation properties. In addition, limited studies have been reported for the transformer oil as a base fluid using Aluminum Oxide (Al2O3) as nanoparticles. Hence, this paper reported an experimental study to investigate the significant role of cold plasma treatment in modifying and treating the surface of nano-alumina to obtain a better interaction between the nano-alumina and the base fluid, consequently improving the insulation characteristics such as breakdown voltage, partial discharge characteristics, thermal conductivity, and viscosity of the nanofluids. The plasma treatment process was conducted on the surface of nano-alumina under atmospheric pressure plasma by using the dielectric barrier discharge concept. The breakdown strength and partial discharge characteristics of the nanofluids were measured according to IEC 60156 and IEC 60270 standards, respectively. In contrast, the viscosity and thermal conductivity of the nanofluids were determined using Brookfield DV-II + Pro Automated viscometer and Decagon KD2-Pro conductivity meter, respectively. The results indicate that the 0.1 wt% of plasma-treated alumina nanofluids has shown the most comprehensive improvements in electrical properties, dispersion stability, and thermal properties. Therefore, the plasma treatment has improved the nanoparticles dispersion and stability in nanofluids by providing stronger interactions between the mineral oil and the nanoparticles.
A Perspective on Modelling Metallic Magnetic Nanoparticles in Biomedicine: From Monometals to Nanoalloys and Ligand-Protected Particles
Authors: Farkaš Barbara , de Leeuw Nora H.
Published: June 2021

The focus of this review is on the physical and magnetic properties that are related to the efficiency of monometallic magnetic nanoparticles used in biomedical applications, such as magnetic resonance imaging (MRI) or magnetic nanoparticle hyperthermia, and how to model these by theoretical methods, where the discussion is based on the example of cobalt nanoparticles. Different simulation systems (cluster, extended slab, and nanoparticle models) are critically appraised for their efficacy in the determination of reactivity, magnetic behaviour, and ligand-induced modifications of relevant properties. Simulations of the effects of nanoscale alloying with other metallic phases are also briefly reviewed.
Flipped Classroom among Minorities in the Context of Mathematics Learning: The Israeli Case
Authors: Sharkia Halima , Kohen Zehavit
Published: June 2021

This study presents a designated flipped classroom (FC) mathematics environment that utilizes a unique online platform designed for Arab minority students in Israel. It investigates how studying in an FC affects conceptual understanding and motivation to study mathematics among Arab high school students. The study also explores the factors that contribute to effective learning in the FC environment. Participants were 75 Arab high school students in 10th and 11th grades who studied advanced mathematics. Each grade group was randomly divided into two subgroups: an FC group and a traditional classroom group (comparison group). Quantitative questionnaires given before and after the learning program served to measure students’ motivation and conceptual understanding of the derivative and integral topics. Additionally, a random sample of students who studied in the FC group and the teacher who taught all the groups were interviewed. The study describes the positive effect an FC environment has on students’ conceptual understanding, particularly for 11th graders. The participants mostly appreciated how the FC resulted in less lecturing in class. The study contributes to the literature about FC among minorities and contributes to national and international efforts being made to reduce the gap in mathematics achievements between minorities and other sectors.
General Fractional Calculus: Multi-Kernel Approach
Authors: Tarasov Vasily E.
Published: June 2021

For the first time, a general fractional calculus of arbitrary order was proposed by Yuri Luchko in 2021. In Luchko works, the proposed approaches to formulate this calculus are based either on the power of one Sonin kernel or the convolution of one Sonin kernel with the kernels of the integer-order integrals. To apply general fractional calculus, it is useful to have a wider range of operators, for example, by using the Laplace convolution of different types of kernels. In this paper, an extended formulation of the general fractional calculus of arbitrary order is proposed. Extension is achieved by using different types (subsets) of pairs of operator kernels in definitions general fractional integrals and derivatives. For this, the definition of the Luchko pair of kernels is somewhat broadened, which leads to the symmetry of the definition of the Luchko pair. The proposed set of kernel pairs are subsets of the Luchko set of kernel pairs. The fundamental theorems for the proposed general fractional derivatives and integrals are proved.
Iterative Design for the Common Solution of Monotone Inclusions and Variational Inequalities
Authors: Wei Li , Shen Xin-Wang , Agarwal Ravi P.
Published: June 2021

Some new forward–backward multi-choice iterative algorithms with superposition perturbations are presented in a real Hilbert space for approximating common solution of monotone inclusions and variational inequalities. Some new ideas of constructing iterative elements can be found and strong convergence theorems are proved under mild restrictions, which extend and complement some already existing work.
A Review of Matrix SIR Arino Epidemic Models
Authors: Avram Florin , Adenane Rim , Ketcheson David I.
Published: June 2021

Many of the models used nowadays in mathematical epidemiology, in particular in COVID-19 research, belong to a certain subclass of compartmental models whose classes may be divided into three “(x,y,z)” groups, which we will call respectively “susceptible/entrance, diseased, and output” (in the classic SIR case, there is only one class of each type). Roughly, the ODE dynamics of these models contains only linear terms, with the exception of products between x and y terms. It has long been noticed that the reproduction number R has a very simple Formula in terms of the matrices which define the model, and an explicit first integral Formula is also available. These results can be traced back at least to Arino, Brauer, van den Driessche, Watmough, and Wu (2007) and to Feng (2007), respectively, and may be viewed as the “basic laws of SIR-type epidemics”. However, many papers continue to reprove them in particular instances. This motivated us to redraw attention to these basic laws and provide a self-contained reference of related formulas for (x,y,z) models. For the case of one susceptible class, we propose to use the name SIR-PH, due to a simple probabilistic interpretation as SIR models where the exponential infection time has been replaced by a PH-type distribution. Note that to each SIR-PH model, one may associate a scalar quantity Y(t) which satisfies “classic SIR relations”,which may be useful to obtain approximate control policies.
Fatty Acid Production and Direct Acyl Transfer through Polar Lipids Control TAG Biosynthesis during Nitrogen Deprivation in the Halotolerant Alga Dunaliella tertiolecta
Authors: Avidan Omri , Malitsky Sergey , Pick Uri
Published: June 2021

The aims of this work were to evaluate the contribution of the free fatty acid (FA) pool to triacylglyceride (TAG) biosynthesis and to try to characterize the mechanism by which FA are assimilated into TAG in the green alga Dunaliella tertiolecta. A time-resolved lipidomic analysis showed that nitrogen (N) deprivation induces a redistribution of total lipidome, particularly of free FA and major polar lipid (PL), in parallel to enhanced accumulation of polyunsaturated TAG. The steady-state concentration of the FA pool, measured by prolonged 14C-bicarbonate pre-labeling, showed that N deprivation induced a 50% decrease in total FA level within the first 24 h and up to 85% after 96 h. The abundance of oleic acid increased from 50 to 70% of total free FA while polyunsaturated FA (PUFA) disappeared under N deprivation. The FA flux, measured by the rate of incorporation of 14C-palmitic acid (PlA), suggests partial suppression of phosphatidylcholine (PC) acyl editing and an enhanced turnover of the FA pool and of total digalactosyl-diacylglycerol (DGDG) during N deprivation. Taken together, these results imply that FA biosynthesis is a major rate-controlling stage in TAG biosynthesis in D. tertiolecta and that acyl transfer through PL such as PC and DGDG is the major FA assimilation pathway into TAG in that alga and possibly in other green microalgae. Increasing the availability of FA could lead to enhanced TAG biosynthesis and to improved production of high-value products from microalgae.
Symbiotic Associations in Ascidians: Relevance for Functional Innovation and Bioactive Potential
Authors: Matos Ana , Antunes Agostinho
Published: June 2021

Associations between different organisms have been extensively described in terrestrial and marine environments. These associations are involved in roles as diverse as nutrient exchanges, shelter or adaptation to adverse conditions. Ascidians are widely dispersed marine invertebrates associated to invasive behaviours. Studying their microbiomes has interested the scientific community, mainly due to its potential for bioactive compounds production—e.g., ET-73 (trabectedin, Yondelis), an anticancer drug. However, these symbiotic interactions embrace several environmental and biological functions with high ecological relevance, inspiring diverse biotechnological applications. We thoroughly reviewed microbiome studies (microscopic to metagenomic approaches) of around 171 hosts, worldwide dispersed, occurring at different domains of life (Archaea, Bacteria, Eukarya), to illuminate the functions and bioactive potential of associated organisms in ascidians. Associations with Bacteria are the most prevalent, namely with Cyanobacteria, Proteobacteria, Bacteroidetes, Actinobacteria and Planctomycetes phyla. The microbiomes of ascidians belonging to Aplousobranchia order have been the most studied. The integration of worldwide studies characterizing ascidians’ microbiome composition revealed several functions including UV protection, bioaccumulation of heavy metals and defense against fouling or predators through production of natural products, chemical signals or competition. The critical assessment and characterization of these communities is extremely valuable to comprehend their biological/ecological role and biotechnological potential.
Research Progress of Chitosan-Based Biomimetic Materials
Authors: Zhang Zhaoyu , Zhang Lingyu , Li Chengpeng , Xie Xiangyu , Li Guangfa , Hu Zhang , Li Sidong
Published: June 2021

Chitosan is a linear polysaccharide produced by deacetylation of natural biopolymer chitin. Owing to its good biocompatibility and biodegradability, non-toxicity, and easy processing, it has been widely used in many fields. After billions of years of survival of the fittest, many organisms have already evolved a nearly perfect structure. This paper reviews the research status of biomimetic functional materials that use chitosan as a matrix material to mimic the biological characteristics of bivalves, biological cell matrices, desert beetles, and honeycomb structure of bees. In addition, the application of biomimetic materials in wound healing, hemostasis, drug delivery, and smart materials is briefly overviewed according to their characteristics of adhesion, hemostasis, release, and adsorption. It also discusses prospects for their application and provides a reference for further research and development.
Effect of Heel-First Strike Gait on Knee and Ankle Mechanics
Authors: Aali Shirin , Rezazadeh Farhad , Badicu Georgian , Grosz Wilhelm Robert
Published: June 2021

Background and Objectives: Acquiring knowledge about the magnitude and direction of induced joint forces during modifying gait strategies is critical for proper exercise prescription. The present study aimed to evaluate whether a heel-first strike pattern during gait can affect the biomechanical characteristics of ankle and knee joints among asymptomatic people. Materials and Methods: In this cross-sectional study performed in the biomechanics laboratory, 13 professional healthy male athletes walked on an instrumented walkway under two walking conditions. For the normal condition, subjects were instructed to walk as they normally would. For the heel-first strike condition, subjects were instructed to walk with heel-first strike pattern and increase heel contact duration as much as possible. Then, knee and ankle joint range of motions and moments, as well as vertical ground reaction force was measured by the Kistler force plate and Vicon motion analysis system. Results: Knee flexion angle at the initial contact and during stance phase was significantly lower when increasing the heel strike pattern. In addition, the mean values of the knee external rotation and adductor moments during heel strike condition were lower than those in normal walking. Further, the ankle dorsiflexion range of motion (ROM) during mid-stance increased significantly during heel-first strike pattern compared to the value in normal gait pattern. Conclusions: The modification of gait pattern including heel-first strike pattern can reduce the mechanical load applied to the knee, while improving the extensibility of gastro-soleus muscle complex.
Cardiac Amyloidosis with Discordant QRS Voltage between Frontal and Precordial Leads
Authors: Eötvös Csilla-Andrea , Lazar Roxana-Daiana , Zehan Iulia-Georgiana , Lévay-Hail Erna-Brigitta , Pastiu Giorgia , Pop Mihaela , Bojan Anca Simona , Pop Sorin , Blendea Dan
Published: June 2021

Among the different types, immunoglobulin light chain (AL) cardiac amyloidosis is associated with the highest morbidity and mortality. The outcome, however, is significantly better when an early diagnosis is made and treatment initiated promptly. We present a case of cardiac amyloidosis with left ventricular hypertrophy criteria on the electrocardiogram. After 9 months of follow-up, the patient developed low voltage in the limb leads, while still maintaining the Cornell criteria for left ventricular hypertrophy as well. The relative apical sparing by the disease process, as well as decreased cancellation of the opposing left ventricular walls could be responsible for this phenomenon. The discordance between the voltage in the frontal leads and precordial leads, when present in conjunction with other findings, may be helpful in raising the clinical suspicion of cardiac amyloidosis.
Exploring the Conformational Changes Induced by Nanosecond Pulsed Electric Fields on the Voltage Sensing Domain of a Ca2+ Channel
Authors: Ruiz-Fernández Alvaro R. , Campos Leonardo , Villanelo Felipe , Gutiérrez-Maldonado Sebastian E. , Perez-Acle Tomas
Published: June 2021

Nanosecond Pulsed Electric Field (nsPEF or Nano Pulsed Stimulation, NPS) is a technology that delivers a series of pulses of high-voltage electric fields during a short period of time, in the order of nanoseconds. The main consequence of nsPEF upon cells is the formation of nanopores, which is followed by the gating of ionic channels. Literature is conclusive in that the physiological mechanisms governing ion channel gating occur in the order of milliseconds. Hence, understanding how these channels can be activated by a nsPEF would be an important step in order to conciliate fundamental biophysical knowledge with improved nsPEF applications. To get insights on both the kinetics and thermodynamics of ion channel gating induced by nsPEF, in this work, we simulated the Voltage Sensing Domain (VSD) of a voltage-gated Ca2+ channel, inserted in phospholipidic membranes with different concentrations of cholesterol. We studied the conformational changes of the VSD under a nsPEF mimicked by the application of a continuous electric field lasting 50 ns with different intensities as an approach to reveal novel mechanisms leading to ion channel gating in such short timescales. Our results show that using a membrane with high cholesterol content, under an nsPEF of 50 ns and E→ = 0.2 V/nm, the VSD undergoes major conformational changes. As a whole, our work supports the notion that membrane composition may act as an allosteric regulator, specifically cholesterol content, which is fundamental for the response of the VSD to an external electric field. Moreover, changes on the VSD structure suggest that the gating of voltage-gated Ca2+ channels by a nsPEF may be due to major conformational changes elicited in response to the external electric field. Finally, the VSD/cholesterol-bilayer under an nsPEF of 50 ns and E→ = 0.2 V/nm elicits a pore formation across the VSD suggesting a new non-reported effect of nsPEF into cells, which can be called a “protein mediated electroporation”.
Polyunsaturated Fatty Acids Mediated Regulation of Membrane Biochemistry and Tumor Cell Membrane Integrity
Authors: Mukerjee Souvik , Saeedan Abdulaziz S. , Ansari Mohd. Nazam , Singh Manjari
Published: June 2021

Particular dramatic macromolecule proteins are responsible for various cellular events in our body system. Lipids have recently recognized a lot more attention of scientists for understanding the relationship between lipid and cellular function and human health However, a biological membrane is formed with a lipid bilayer, which is called a P–L–P design. Our body system is balanced through various communicative signaling pathways derived from biological membrane proteins and lipids. In the case of any fatal disease such as cancer, the biological membrane compositions are altered. To repair the biological membrane composition and prevent cancer, dietary fatty acids, such as omega-3 polyunsaturated fatty acids, are essential in human health but are not directly synthesized in our body system. In this review, we will discuss the alteration of the biological membrane composition in breast cancer. We will highlight the role of dietary fatty acids in altering cellular composition in the P–L–P bilayer. We will also address the importance of omega-3 polyunsaturated fatty acids to regulate the membrane fluidity of cancer cells.
Modification of Ceramic Membranes with Carbon Compounds for Pharmaceutical Substances Removal from Water in a Filtration—Adsorption System
Authors: Polak Daniel , Zielińska Izabela , Szwast Maciej , Kogut Igor , Małolepszy Artur
Published: June 2021

The aim of this work is to develop a new type of carbon-ceramic membranes for the removal of pharmaceutical substances from water. The membranes were prepared by the chemical modification method using an organosilicon precursor—octadecyltrichlorosilane (ODTS). Graphene oxide, multi-walled carbon nanotubes with carboxylic groups, and single-walled carbon nanotubes were used in the modification process. The filtration properties and adsorption properties of the developed membranes were tested. In order to characterize the membrane, the water permeability, the change of the permeate flux in time, and the adsorbed mass of the substance were determined. Additionally, the surface properties of the membranes were characterized by contact angle measurements and porosimetry. The antibiotic tetracycline was used in the adsorption tests. Based on the results, the improved adsorption properties of the modified membrane in relation to the unmodified membrane were noticed. Novel ceramic membranes modified with MWCNT are characterized by 45.4% removal of tetracycline and permeate flux of 520 L·h·m−2·bar−1. We demonstrated the ability of modified membranes to adsorb pharmaceuticals from water streams that are in contact with the membrane. Novel membranes retain their filtration properties. Therefore, such membranes can be used in an integrated filtration–adsorption process.
Surface Micromorphology and Structure of Stainless and Maraging Steel Obtained via Selective Laser Melting: A Mössbauer Spectroscopy Study
Authors: Linderhof Fredericus , Mashlan Miroslav , Doláková Hana , Ingr Tomáš , Ivanova Tatiana
Published: June 2021

Selective laser melting (SLM) as an additive manufacturing method makes it possible to quickly produce complexly shaped three-dimensional (3D) metal specimens from a powder. This work describes how SLM affects the surface phase composition of a 3D printed specimen, as analyzed with conversion electron Mössbauer spectroscopy (CEMS), conversion X-ray Mössbauer spectroscopy (CXMS) and X-ray diffraction (XRD). Both stainless 1.4404 (CL20ES) steel and maraging 1.2709 (CL50WS) steel have been investigated. A transformation of the phase composition from the ferritic phase into an austenitic one was proven by comparing the initial CL50WS powder and the final specimen using CXMS. This transformation takes place during the SLM process. No transformation was identified in stainless steel. The differences identified via CEMS between the surface phase composition of the final non-annealed specimens and the surface of the final annealed specimens demonstrated the oxidation of the surface layer. The oxidation occurs during the annealing of the sample in surface layers less than 1 μm thick. The quality of the surface was examined using scanning electron microscopy (SEM), which presented imperfections on the face of the final specimen. Granules of the initial powder bonded to the surface of the specimen and both irregular and spherical pores were observed.
Dephosphorization in Double Slag Converter Steelmaking Process at Different Temperatures by Industrial Experiments
Authors: Sun Han , Yang Jian , Lu Xinwu , Liu Wanshan , Ye Gefan , Zhang Runhao , Yang Wenkui
Published: June 2021

In the present work, the effect of dephosphorization endpoint temperature on the dephosphorization of hot metal was studied for the double slag converter steelmaking process under the conditions of low temperature and low basicity by the industrial experiments. In the temperature range of 1350–1450 °C, with an increasing dephosphorization endpoint temperature, the dephosphorization ratio and phosphorus distribution ratio first increase and then decrease. The phosphorus content in hot metal first decreases and then increases at the end of dephosphorization. At the dephosphorization temperature range of 1385–1410 °C, the dephosphorization ratio is higher than 55%, the P2O5 content in the dephosphorization slag is 3.93–4.17%, the logLP value is 1.76–2.09, the value of PCOP-C of the selective oxidation reaction of carbon and phosphorus is 53–80 Pa, and the aFeO value is 0.284–0.312. The path of phosphorus in hot metal entering the P-rich phase of dephosphorization slag can be reasonably inferred as: hot metal → Fe-rich phase → P-rich phase. Under the present industrial experimental conditions, the dephosphorization and rephosphorization reactions are in dynamic equilibrium at 1413 °C. Considering the experimental results and thermodynamic calculation results of industrial experiments by the double slag dephosphorization process, the optimal temperature range for intermediate deslagging is about 1400–1420 °C.
Formation Process of Long-Period Stacking-Ordered Structures in Mg97Zn1Y2 Alloy Comprising HCP and Cubic Phases Fabricated by High-Pressure High-Temperature Annealing
Authors: Yokota Atsuki , Matsushita Masafumi , Geshi Naruhito , Yamasaki Daiki , Shinmei Toru , Yamasaki Michiaki , Kawamura Yoshihito
Published: June 2021

As-cast Mg97Zn1Y2 alloy consists of α-Mg matrix and 18R-type long-period stacking-ordered (LPSO) structures. We observed that the alloy undergoes a phase transformation to D03 superlattices and α-Mg matrix due to high-pressure high-temperature (HPHT) annealing at 3 GPa and above 773 K. Further, the alloy recovered after HPHT annealing, consisting of the α-Mg matrix and D03 superlattices, transformed into 18R-type LPSO structures during further annealing at ambient pressure. An fcc structure with a lattice parameter of 1.42 nm, which was twice that of D03, emerged in both the collapse process of the 18R-type LPSO structure under high-pressure, and the formation process of the 18R-type LPSO structure at ambient pressure. This fcc phase was an intermediate structure between 18R-type LPSO and D03. From the electron diffraction results, it is considered that 18R-type LPSO is continuously present with 2H including stacking faults, which almost corresponded with previous studies.
Study on the Effect of Heat Treatment on Microstructures and High Temperature Mechanical Properties of Welding Spots of Hot Stamped Ultra-High Strength Steel Patchwork Blanks
Authors: Ouyang Xiao , Zhang Zhiqiang , Jia Hongjie , Ren Mingwen , Sun Yaping
Published: June 2021

Insufficient strength of welding spots is a common problem in the hot stamping process of ultra-high strength steel patchwork blanks (UHSSP). In this paper, the welding spots of 22MnB5 boron steel with thicknesses of 1.2 and 1.5 mm were austenitized and then air-cooled to 650–850 °C for high temperature tensile shear tests and high temperature cross-tension tests, respectively. To study the mechanical properties of the welding spots at room temperature after heat treatment, the austenitized welding spots were quenched in cold water to room temperature, and microhardness tests and microstructure observations were performed. The results indicated that compared to the original welding spots, the heat-affected softening zone disappeared after heat treatment, and the hardness values of the fusion zone, heat-affected zone and base material were basically the same, at about 500 HV. After heat treatment, the welding spots were mainly martensite. With the increase in deformation temperature, the peak loads of the tensile shear and the cross tension of the welding spots decreased. At 750 °C, the peak loads of the welding spots decreased less, energy absorption was larger, and the welding spots had the comprehensive mechanical properties of strength and ductility.
Efficiency and Sustainability Analysis of the Repair and Maintenance Operations of UNS M11917 Magnesium Alloy Parts of the Aeronautical Industry Made by Intermittent Facing
Authors: Fernández Jacobo , Rubio Eva María , Carou Diego , Lorente-Pedreille Raquel María
Published: June 2021

This paper analyzes the efficiency and sustainability of facing operations that are required within maintenance operations in the aeronautical industry. Due to the elevated cost and environmental impact of such processes, reducing the operating time while repairing parts is required. In this work, an experimental study of intermittent facing carried out on a magnesium alloy rod was developed. The experiment resembles real repair and maintenance machining operations, where an intermittent facing represents a more realistic scenario and where the results obtained in continuous turning studies are not always applicable. The work was performed with different cooling and lubrication systems and various cutting conditions, also considering the size of the interruption to analyze their impact in the surface roughness. To this end, surface finished in different measuring zones was studied. The aims of the study are to get a better understanding of the intermittent facing process in magnesium alloys typically employed in aeronautical applications and find the most efficient cutting parameters to obtain an improved surface under the safest and most environmentally respectful conditions.
Defining the Relative Role of Insulin Clearance in Early Dysglycemia in Relation to Insulin Sensitivity and Insulin Secretion: The Microbiome and Insulin Longitudinal Evaluation Study (MILES)
Authors: Wood Alexis C. , Jensen Elizabeth T. , Bertoni Alain G. , Ramesh Gautam , Rich Stephen S. , Rotter Jerome I. , Chen Yii-Der I. , Goodarzi Mark O.
Published: June 2021

Insulin resistance and insufficient insulin secretion are well-recognized contributors to type 2 diabetes. A potential role of reduced insulin clearance has been suggested, but few studies have investigated the contribution of insulin clearance while simultaneously examining decreased insulin sensitivity and secretion. The goal of this study was to conduct such an investigation in a cohort of 353 non-Hispanic White and African American individuals recruited in the Microbiome and Insulin Longitudinal Evaluation Study (MILES). Participants underwent oral glucose tolerance tests from which insulin sensitivity, insulin secretion, insulin clearance, and disposition index were calculated. Regression models examined the individual and joint contributions of these traits to early dysglycemia (prediabetes or newly diagnosed diabetes). In separate models, reduced insulin sensitivity, reduced disposition index, and reduced insulin clearance were associated with dysglycemia. In a joint model, only insulin resistance and reduced insulin secretion were associated with dysglycemia. Models with insulin sensitivity, disposition index, or three insulin traits had the highest discriminative value for dysglycemia (area under the receiver operating characteristics curve of 0.82 to 0.89). These results suggest that in the race groups studied, insulin resistance and compromised insulin secretion are the main independent underlying defects leading to early dysglycemia.
Sigma-1 Receptor Promotes Mitochondrial Bioenergetics by Orchestrating ER Ca2+ Leak during Early ER Stress
Authors: Koshenov Zhanat , Oflaz Furkan E. , Hirtl Martin , Pilic Johannes , Bachkoenig Olaf A. , Gottschalk Benjamin , Madreiter-Sokolowski Corina T. , Rost Rene , Malli Roland , Graier Wolfgang F.
Published: June 2021

The endoplasmic reticulum (ER) is a complex, multifunctional organelle of eukaryotic cells and responsible for the trafficking and processing of nearly 30% of all human proteins. Any disturbance to these processes can cause ER stress, which initiates an adaptive mechanism called unfolded protein response (UPR) to restore ER functions and homeostasis. Mitochondrial ATP production is necessary to meet the high energy demand of the UPR, while the molecular mechanisms of ER to mitochondria crosstalk under such stress conditions remain mainly enigmatic. Thus, better understanding the regulation of mitochondrial bioenergetics during ER stress is essential to combat many pathologies involving ER stress, the UPR, and mitochondria. This article investigates the role of Sigma-1 Receptor (S1R), an ER chaperone, has in enhancing mitochondrial bioenergetics during early ER stress using human neuroblastoma cell lines. Our results show that inducing ER stress with tunicamycin, a known ER stressor, greatly enhances mitochondrial bioenergetics in a time- and S1R-dependent manner. This is achieved by enhanced ER Ca2+ leak directed towards mitochondria by S1R during the early phase of ER stress. Our data point to the importance of S1R in promoting mitochondrial bioenergetics and maintaining balanced H2O2 metabolism during early ER stress.
Altered Ca2+ Handling and Oxidative Stress Underlie Mitochondrial Damage and Skeletal Muscle Dysfunction in Aging and Disease
Authors: Michelucci Antonio , Liang Chen , Protasi Feliciano , Dirksen Robert T.
Published: June 2021

Skeletal muscle contraction relies on both high-fidelity calcium (Ca2+) signals and robust capacity for adenosine triphosphate (ATP) generation. Ca2+ release units (CRUs) are highly organized junctions between the terminal cisternae of the sarcoplasmic reticulum (SR) and the transverse tubule (T-tubule). CRUs provide the structural framework for rapid elevations in myoplasmic Ca2+ during excitation–contraction (EC) coupling, the process whereby depolarization of the T-tubule membrane triggers SR Ca2+ release through ryanodine receptor-1 (RyR1) channels. Under conditions of local or global depletion of SR Ca2+ stores, store-operated Ca2+ entry (SOCE) provides an additional source of Ca2+ that originates from the extracellular space. In addition to Ca2+, skeletal muscle also requires ATP to both produce force and to replenish SR Ca2+ stores. Mitochondria are the principal intracellular organelles responsible for ATP production via aerobic respiration. This review provides a broad overview of the literature supporting a role for impaired Ca2+ handling, dysfunctional Ca2+-dependent production of reactive oxygen/nitrogen species (ROS/RNS), and structural/functional alterations in CRUs and mitochondria in the loss of muscle mass, reduction in muscle contractility, and increase in muscle damage in sarcopenia and a wide range of muscle disorders including muscular dystrophy, rhabdomyolysis, central core disease, and disuse atrophy. Understanding the impact of these processes on normal muscle function will provide important insights into potential therapeutic targets designed to prevent or reverse muscle dysfunction during aging and disease.
Betatrophin Levels Are Related to the Early Histological Findings in Nonalcoholic Fatty Liver Disease
Authors: Sonmez Alper , Dogru Teoman , Ercin Cemal Nuri , Genc Halil , Celebi Gurkan , Gurel Hasan , Tapan Serkan , Cicek Ali Fuat , Barcin Cem , Haymana Cem , Kirik Ali , Rizzo Manfredi
Published: June 2021

Betatrophin, a liver hormone, regulates glucose and lipid metabolism. We investigated the betatrophin levels in nonalcoholic fatty liver disease (NAFLD) and searched for any relationship with histological severity and metabolic parameters. Fifty males with NAFLD [Nonalcoholic Steatohepatitis (NASH) (n = 32); non-NASH (n = 18)] and 30 healthy controls were included. Plasma betatrophin was measured by ELISA method. Insulin sensitivity was assessed by HOMA-IR index. Histological features were scored by the semi quantitative classification and combined as the NAFLD activity score (NAS). Betatrophin levels in the non-NASH group were significantly higher than the controls. Betatrophin was positively correlated to the age, waist circumference, total cholesterol, triglycerides, LDL cholesterol, glucose, insulin, HOMA-IR index and gamma glutamyl transpeptidase levels, and negatively correlated to the steatosis and NAS. In the stepwise linear regression analysis, the triglyceride (β = 0.457, p < 0.001), glucose (β = 0.281, p = 0.02) and NAS (β = −0.260, p = 0.03) were the independent determinants of betatrophin. Betatrophin levels are higher in the early stages of NAFLD and tend to decrease when the disease progresses. This could be an important preliminary mechanistic finding to explain the increased frequency of glucose intolerance during the course of NAFLD.