bluuebunny/crossref_metadata_2025 huggingface dataset:
Uso da técnica push-out para análise da resistência ao cisalhamento da colagem com materiais retro-obturadores
Authors: Araújo José Duylles da Silva , Almeida Wanderson Carvalho de , Silva Humbelina Alves da , Diniz Natrícia Sousa , Castro Fernando Antônio Pontes , Barbosa Rodrigo Barcelos , Lacerda Aniele Carvalho , Ferraz Maria Ângela Arêa Leão , Falcão Carlos Alberto Monteiro
Published: August 2023

Introdução: O tratamento de escolha para dentes com porção radicular contaminada é o tratamento endodôntico; contudo, quando há falha nesse tratamento, é necessário realizar o retratamento do canal radicular. A cirurgia parendodôntica é um dos meios de retratamento, possibilitando uma manutenção do dente na cavidade bucal. A técnica push-out visa mensurar a força de resistência ao cisalhamento entre cimento e dentina. Objetivo: O objetivo do presente estudo foi avaliar, usando a técnica push-out, a resistência ao cisalhamento da colagem com materiais retro-obturadores. Métodos: Foram selecionados 21 pré-molares inferiores com raízes completamente formadas, livres de trincas, fraturas e curvaturas acentuadas, com canal único e ausência de calcificações. As coroas de todos os dentes foram removidas e as raízes, seccionadas transversalmente com máquina de corte de precisão. As cavidades realizadas nas secções foram divididas em três grupos e preenchidas com os materiais, sendo: Grupo 1) MTA Angelus Branco, Grupo 2) MTA Angelus Repair HP e Grupo 3) Cimento de Portland. Após a obturação com os materiais retro-obturadores, as secções foram submetidas ao teste de cisalhamento. Resultados: O MTA Angelus Branco apresentou a maior resistência ao cisalhamento entre os materiais analisados. Não houve diferença estatística entre o MTA Angelus Repair HP e o Cimento de Portland. O MTA Angelus Repair HP e o Cimento de Portland não apresentaram diferença estaticamente significativa quanto à resistência ao cisalhamento e ao tipo de falha apresentada. Conclusões: O MTA Angelus Branco mostrou melhor adesão às paredes dentinárias.
Reimplante tardio com acompanhamento de seis anos: relato de caso
Authors: Santos Carolina Nader , Sousa Érica Luiza S. M. , Sousa Kássio Henrique , Pereira Renato Piai , Silva Rogério Vieira
Published: August 2023

Introdução: A avulsão dentária é um problema de saúde pública e tem alta prevalência. A mais previsível conduta a ser seguida é o reimplante imediato. Objetivo: Relatar um caso clínico de avulsão dentária do dente #21 com reimplante tardio no período de 24 horas. Descrição: Paciente do sexo feminino, nove anos de idade, procurou avaliação odontológica. A mãe informou que a criança havia sofrido uma queda e que estava com o dente acondicionado em soro fisiológico. Ela ainda informou que levou a criança ao hospital; porém, nenhuma conduta foi realizada em relação ao dente avulsionado. Após 24h do traumatismo dentário, antes do reimplante, realizou-se o acesso coronário e pulpectomia em meio extra-alveolar. Realizou-se o reimplante e contenção semirrígida com fio ortodôntico de aço 0,2 mm fixado com resina composta da região dos dentes #22 a #12. O dente foi instrumentado pela técnica crown-down até o comprimento de trabalho (CT) com a lima tipo K #55, medicação intracanal com Downs-Calen por 21 dias e obturação do canal radicular pela técnica termoplastificada, associada ao cimento endodôntico AH Plus. O dente foi restaurado com resina composta fotopolimerizável. Os acompanhamentos clínicos e radiográficos foram semestrais. Resultados: O protocolo de tratamento proposto foi eficaz. Constatou- se ausência de sinais e sintomas, com tecidos moles dentro dos padrões de normalidade. Conclusão: O dente encontra-se com anquilose e reabsorção por substituição; porém, não há mobilidade nem doença periodontal, e a paciente encontra-se em acompanhamento clínico e radiográfico em um período de seis anos em condições satisfatórias.
Planungskonflikte in der pluralistischen Demokratie
Authors: Hesse Markus , Kühn Manfred
Published: August 2023

This paper deals with planning conflicts in the pluralistic democracy, based on an international literature review. The aim is to trace the state of research on how spatial planning deals with conflicts. Moreover, we reflect upon the development of major planning paradigms with regard to this topic. To this end, some basics on conflict and conflict resolution in spatial planning are presented. The collaborative planning paradigm, which appears hegemonic in planning theory and practice until today, is then critically discussed with regard to its handling of conflicts. Subsequently, agonistic planning theory is introduced with reference to the work of political scientist Chantal Mouffe. This concept claims to deal specifically with conflict, which renders it particularly relevant for this topic. In conclusion, the paper reflects on the extent to which agonistic planning can have practical relevance for the regulation of conflicts and how this can be achieved in contemporary planning practice.
MEHMET ÂKİF ERSOY’UN MUHAFAZAKÂRLIĞA YÖNELİK TEMEL FİKİRLERİ
Authors: NÜKTE Serdar
Published: August 2023

Bu makalenin amacı, Türk düşünce hayatında önemli bir isim olan Mehmet Âkif Ersoy’un muhafazakârlığa ilişkin görüşlerini tahlil etmektir. Muhafazakâr düşünce, dine, tarihsel birikime, geleneğe, değişim konusuna ve toplumsal değerlerin devam edilmesi gerektiğine ilişkin yaklaşımlarıyla ön plana çıkan bir düşünce sistemidir. Mehmet Âkif Ersoy da, bu düşünce sisteminden hareketle, bir toplumun kendi tarihine kaynaklık eden değerleri muhafaza ederek, değişim sürecine girmesi gerektiğine inanmıştır. Bu nedenle, yaşadığı dönemin sorunlarını ele alırken, toplumsal değerlerin muhafaza edilmesini önemsemiştir. Böylelikle, modernleşmeye ve değişime bağlı olarak bir toplum analizi yapmış ve bu doğrultuda ortaya çıkan taklit, tarihsel umursamazlık, milliyetçilik, aydın halk çatışması, atalet, bid’at ve hurafe gibi konular hakkında değerlendirmeler yapmıştır. Mehmet Âkif Ersoy, bu değerlendirmelerinde toplumun geçmişinde var olan değerleri yeniden ortaya çıkarma çabasında olmuştur. Bu makalede, Mehmet Âkif Ersoy’un muhafazakârlık konusundaki fikirleri, Safahat adlı eseri, tefsir yazıları, mektupları ve halkı aydınlatmak için vermiş olduğu vaazlardan hareketle analiz edilmiştir.
HASTALIK VE KÜLTÜR İLİŞKİSİNE KURAMSAL BAKIŞ
Authors: GÜNGÖR Sümeyye , KAYA Elif
Published: August 2023

Kültür, geçmişten günümüze değişerek gelen, kendine özgü sanatı, inancı, örf, adeti ve davranışlarıyla toplumun kimliğini oluşturan düşünce tarzıdır. Bu yönüyle, toplumda ortaya çıkarılan, değer verilen, paylaşılan tüm maddi ve manevi ögeleri kapsamaktadır. Kültür, nasıl algılayacağı ya da davranacağı konusunda bireye yol göstermektedir. Bu noktada, bireyin karşılaştığı hastalık durumlarının algılanması ve açıklanmasında kültür, önemli bir noktaya sahiptir. Hastalığa yakalanmak, hastalığı yaşamak, hastalık rollerini taşımak, hastalıkla baş etmeye çalışmak, hastalıktan şikayetlenmek gibi tüm aşamalarda kültürün belirleyici bir rolü vardır. Dolayısıyla bu çalışmada kültür ile hastalık arasındaki etkileşimi teorik olarak incelemek amaçlanmıştır. Çalışmanın sonucunda, bireylerin kültüre göre hastalıklar üzerinde bilgi sahibi olmaya çalıştıkları ve hastalığa yönelik davranışlar geliştirdikleri görülmektedir. Bu açıdan kültürün plasebo ve nosebo etkisi yarattığı sonucuna ulaşılmıştır. Plasebo etkisi bireyler üzerinde iyileştirici güç olarak algılanırken, nosebo etkisinin bireyler üzerinde patolojik durum yarattığı görülmektedir. Bu nedenle sağlık profesyonellerinin sağlık hizmeti sunarken, bireylerin kültürlerini göz önüne alarak hastalığın kaynağını saptaması önemlidir.
Haplotype and phylogenetic diversity using mitochondrial 12S rRNA gene marker in Bali cattle
Authors: Suselowati T. , Noor R. R. , Jakaria J.
Published: August 2023

Bali cattle is one of Indonesia's original livestock genetic resources. The 12S rRNA can be used as a marker of genetic diversity, and until now, there has been no report on Bali cattle. The study was aimed to analyze the gene of mtDNA 12S rRNA to determine haplotype diversity and phylogenetics in Bali cattle populations of the eastern region of Indonesia. A total of 95 blood samples used consisted of three different populations which were Bali cattle, Ongole crossbred (PO) and Madura cattle as a comparison. This research was analyzed using PCR and sequencing methods. The data were analyzed using the cluster W method for estimating genetic distances, calculating diversity, and reconstructing phylogenetic trees using the MEGAX, DNAsP and Network. The genetic distance values ranged from 0.00200 to 0.01508, and the Haplotype diversity values ranged from 0.66000 to 0.91111. The nucleo-tide diversity values ranged from 0.00174 to 0.01673. There were 16 haplotypes found. The values of Gst, Nst and Fst were 0.00803, 0.07550, 0.07622; respectively. Based on the analysis, there were dif-ferences between Bali cattle from various populations, there were specific haplotypes. The 12S rRNA gene can be used as a genetic marker for diversity studies in Bali cattle and other cattle breeds although the diversity is low.
A comparative study on histopathological features of duodenum, jejunum, and ileum from broiler chicken with avian pathogenic Escherichia coli infection
Authors: Dameanti F. N. A. E. P. , Dacosta A. O. , Adrenalin S. L. , Fatmawati M. , Permata F. S. , Siswanto H. P. , Ariyanti T.
Published: August 2023

Avian colibacillosis is a high-prevalence chicken farm disease caused by avian pathogen-ic Escherichia coli (APEC). It is necessary to identify and look up to the bacterial activity to stave off a decline in chicken performance. This study aimed to identify and determine the pathogenetic activity of APEC within broiler chickens on the duodenum, jejunum, and ileum histopathology comparison. The samples were collected from a total of 40 broilers, obtained from a highest-colibacillosis historical chicken house, identified with bacterial isolation swab-technique, and analyzed with nonparametric statistical lesions scoring. The identification result showed 82.5% E. coli with a 62.5% APEC con-firmed. The histopathological feature showed a significant difference (p<0.05), where the jejunum ap-peared to encounter the most significant damage compared to the duodenum and ileum. Thus, it is con-cluded that APEC field infection varies in characteristics and pathogenicity, which affects intestinal organs based on the histology changes, mainly in the jejunum part of the intestine.
Pengaruh Price Dan Social Media Marketing Activities (SMMA) Terhadap Healthcare Apps Purchase Intention Dimediasi Oleh Consumption Values
Authors: Alverina Claudia , Achmadi Hendra
Published: August 2023

The increasing use of social media in Indonesia opens up opportunities for entrepreneurs especially in the health sector on COVID-19 pandemic era. During the COVID-19 pandemic, the need for online health services has soared. According to data from the Global Web Index (GWI) social media users in Indonesia in 2022 increased by 12.6% in the past year. The number of rivalries between health facilities is getting tougher, so a specific strategy is needed in marketing techniques in the field of health services in Indonesia. Market price competition is also one of the problems in the business sector. In this study, 163 samples collected had purchased health care products on the MySiloam application belonging to Siloam Hospital Group. The sample was obtained by purposive sampling method and then the data was processed using the PLS-SEM analysis method with the SmartPLS ver 3.2.9 application. The results obtained from data processing, there are several hypotheses that do not show a relationship between research variables, but social media marketing activities (SMMA) have the strongest influence on epistemic value which then increases healthcare apps purchase intention. Suggestions for further research should be done with a wider number of samples and tighten the criteria for respondents so that the data obtained is more significant and accurate.
Factors Associated with The Incidence of Pulmonary Tuberculosis in Lebak District, Banten Province, Indonesia
Authors: Lubis Rosmawaty , Hisni Dayan , Rahmatullah Bobby Rizky
Published: August 2023

Introduction: Pulmonary tuberculosis (TB) is a chronic disease caused by Mycobacterium tuberculosis. The prevalence of pulmonary TB is increasing every year. The previous studies found that knowledge, physical condition of the house, gender and education level are the well-known of factors associated with the TB cases. This study aims to determine the factors associated with the incidence of Pulmonary TB at Puskesmas Maja, Lebak Regency, Banten Province, Indonesia.Methods: Case control study design was used in this study. Total 20 cases and controls were recruited. Cases are individuals aged over 15 years with pulmonary tuberculosis who were confirmed by sputum examination with acid-fast staining (BTA+) showing the presence of Mycobacterium tuberculosis. Controls were individuals with negative sputum examination results. A purposive sampling technique was used to find the defined-case and control groups. A study questionnaire was used to collect the determinant factors and house condition parameter was measured directly. The chi-square test was used to determine the association of each variable related to TB incidence.Results: The demographic data showed that the majority of TB patients in Puskesmas Maja were male who had an elementary to high school education level and had a low level of knowledge and unhealthy physical conditions of the house. The results of bivariate analysis showed that there was a significant relationship between gender, education level, knowledge, and physical condition of the house with the incidence of pulmonary TB at Puskesmas Maja, Lebak Regency.Conclusion: This study concludes that all four variables have a significant relationship with the occurrence of Pulmonary TB at Puskesmas Maja, Lebak Regency. The results of this study can be a source of reference for further research and also become the basis for overcoming pulmonary TB in Banten Province in particular and in Indonesia
Recenze: Adam Csukás: Právní řád Českobratrské církve evangelické v historických souvislostech
Authors: Pfann Michael
Published: August 2023

Recenze: Adam Csukás: Právní řád Českobratrské církve evangelické v historických souvislostech
Dominant Nucleolus in the Progenitor Cell Using Human Bone Marrow Erythroid and Granulocytic Cell Lineages as a Model. A Morphological and Cytochemical Note
Authors: Smetana Karel , Klamová H. , Mikulenková D.
Published: August 2023

Progenitor cells of the human erythroid and granulocytic cell lineages are characterized by the presence of several nucleoli. One of these nucleoli is larger and possesses more fibrillar centres than others. Such nucleolus is apparently dominant in respect of both size and main nucleolar function such as nucleolar-ribosomal RNA transcription. Such nucleolus is also visible in specimens using conventional visualization procedures, in contrast to smaller nucleoli. In the terminal differentiation nucleated stages of the erythroid and granulocytic development, dominant nucleoli apparently disappeared, since these cells mostly contained very small nucleoli of a similar size with one fibrillar centre. Thus, the easily visible dominant nucleoli appear to be useful markers of the progenitor cell state, such as proliferation, and differentiation potential.
Combination of exploitation systems to optimize yield of GT1 clone in smallholder rubber (Hevea brasiliensis) plantation in Sumatera Utara, Indonesia
Authors: Purwaningrum Yayuk , Asbur Yenni , Satriawan Halus , Sari Murni , Nurhayati
Published: August 2023

Clone GT1 is one of the low metabolism clones and is recommended for smallholder and large plantations. These clones are responsive to stimulants and reach production peak at the mid-economic cycle period. Its production can reach 2200-3000 kg-1ha-1year-1 and is relatively more resistant to high exploitation pressures if the agroecosystem conditions are suitable. This study aimed to determine the effect of the combined application of liquid stimulant concentration and tapping interval on the physiology and productivity of latex in rubber clone GT1. This research was conducted at Naga Rejo Village, Galang District, Regency of Deli Serdang, Province of Sumatera Utara, located at an altitude of 49 masl with Ultisol soil type. The research design is factorial randomized completed block design (RCBD) consisting of 9 levels, namely S2d4 without a stimulant, S2d4 ET 3.5%, S2d4 ET 4.5%, S2d5 without a stimulant, S2d5 ET 3.5%, S2d5 ET 4.5%, S2d6 without a stimulant, S2d6 ET 3.5%, S2d6 ET 4.5 % with three replicas of each treatment. The results showed that the tapping system on clone GT1 obtained the highest annual production with tapping system S2d4 ET 4.5% (DS3) of 214.51 g/t/y. The lowest production is obtained in January-March when the leaves fall. High sucrose levels in the non-stimulant treatment (8.54 -12.98 mM) indicated that at least sucrose was converted into latex. The pH of latex is directly proportional to the stimulant, so an increase in the concentration of the stimulant raises the pH and metabolism of latex formation.
Mechanisms of Exocytosis
Authors: Vardjan Nina , Stenovec Matjaž , Jorgačevski Jernej , Kreft Marko , Zorec Robert
Published: August 2023

Vesicles are cellular organelles, in which signaling molecules (neurotransmitters or hormones) are stored and are essential for the function of neurons and endocrine cells in supporting the communication between tissues and organs. Upon stimulation the signaling molecules stored inside vesicles are released from cells by exocytosis. This fundamental biological process consists of membrane fusion between the vesicles and the plasma membrane, leading to the formation of an aqueous channel – the fusion pore – through which signaling molecules exit into the extracellular space or blood stream. The vesicle cargo discharge initially requires the delivery of vesicles to the plasma membrane, where vesicles dock and get primed for fusion with the plasmamembrane. Classical view holds that stimulation initiates the fusion pore formation and vesicle cargo discharge in an all-or-none fashion. Once formed the fusion pore may close (transient, “kiss-and-run” exocytosis) or expand, leading to the full collapse of the vesicle membrane into the plasma membrane (full fusion exocytosis). However, recent studies indicate that exocytosis may not be as simple. Here we highlight the novel findings which indicate that transient fusion pore is subject to regulations, which affect the release competence of a single vesicle. Our recent studies have shown that in pituitary lactotrophs vesicle release of peptide signaling molecules involves modulation of fusion pore kinetics and fusion pore conductance.
Juncus atratus Krock. (Juncaceae) rediscovered in Slovenia
Authors: Lainšček Tadej , Bačič Tinka , Jogan Nejc
Published: August 2023

Juncus atratus Krock. of the Juncaceae family is a Central European-Southern Siberian wet-meadow species. In Central Europe, the species is very rare and threatened. In Slovenian Red Data List, it is listed among unsufﬁciently known species (K). The only record of the species in Slovenian territory (Prem near Ilirska Bistrica, SW Slovenia) is over 100 years old and has never been conﬁrmed afterwards. In May 2010, the species was found in Goričko (NE Slovenia), were it thrives on a wet meadow near Kobilje village. The article discusses the recent ﬁnding of the species in Slovenia in broader context, its distribution, habitat and threat factors. The aim of the study was also to provide bases for nature-conservation of the species in Slovenia. According to recent ﬁnding and the results of the study, the authors suggest Juncus atratus to be regarded as ‘endangered’ (E) in the national red data list.
Za vedno je odšel Tone Wraber
Authors: Jorgan N. , Bačič T.
Published: August 2023

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Wet meadows with Purple Moor-grass (Molinia caerulea) in Slovenia
Authors: Zelnik Igor
Published: August 2023

The paper presents wet meadow vegetation with taxon Molinia caerulea (L.) Moench subsp. caerulea in Slovenia. The main objective of this study was to examine the plant species composition and plant species richness of wet meadow plantcommunities with the mentioned dominating or co-dominating plant taxon. Vegetation was recorded in accordance with standard Central European method. Vegetation types were classified by means of multivariate analysis. Four associations from the alliance Molinon Koch 1926 were identified and analysed: Plantagini altissimae-Molinietum caeruleae Marchiori & Sburlino 1982, Selino-Molinietum caeruleae Kuhn 1937, Carici davallianae-Molinietum caeruleae Špániková 1978 and Junco-Molinietum caeruleaePreising 1951 ex Klapp 1954. Ecological characteristics, plant species composition and richness of the delimited plant communities are presented, as well as their syntaxonomic position and distribution. For two of the mentioned communities relevés made in Slovenia are published here for the first time.
Localities and sites of protected and endangered species Bellevalia romana (L.) Reichenb. (Hyacinthaceae) in Slovenia
Authors: Dakskobler Igor , Vreš Branko
Published: August 2023

The article describes three new localities of a rare and endangered species of lowland meadows Bellevalia romana in Slovenia: at Ankaran in Slovenian Istria (confirmed in 2002, but not found yet in 2011) and at Golo Brdo and between Golo Brdo and Mišček in the Idrija valley (Goriška Brda). Only a total of four localities of this species can be confirmed in Slovenia in the year 2011 – in the Goriška region also under Sv. Katarina above Kromberk and in Istria on more places at Sečovlje. As regards their sites, the localities in the Goriška region on flysh and riparian meadows differ from those in Istria, where it grows on wet meadows and in reed and tall herb communities, on trampled soil in the immediate vicinity of the sea. The endangered species can be preserved through adequate protection of its sites. That usually means annual, but not too early, mowing of the meadows on which it grows.
Response of two submersed macrophytes Ceratophyllum demersum and Myriophyllum spicatum to selenium in water
Authors: Mechora Špela , Stibilj Vekoslava , Germ Mateja
Published: August 2023

Two submersed macrophytes (Ceratophyllum demersum and Myriophyl- lum spicatum) were exposed to water containing 10 mg Se(IV) L–1, later transferred to water without Se and exposed again to 10 mg Se(IV) L–1 with the aim to observe the recovery of plants. After each transplantation trial, potential photochemical efficiency of photosystem II, respiratory potential and the amount of photosynthethic pigments and anthocyanins were measured. Photochemical efficiency was similar in all three trials. Electron transport system (ETS) activity increased dramatically in C. demersum plants that were transferred from the water with Se to the water without Se, while ETS activity strongly increased in M. spicatum specimens, when the second time transferred to water containing Se. Alternation in the concentration of Se in the growth media demanded metabolic changes in studied plants. The amount of chlorophylls was higher in plants of M. spicatum growing in water without Se than in exposed plants, while the amount of carotenoids and anthocyanins decreased in the same species grew in water without Se. The amount of Se was higher in plants exposed to Se, while plants that grew in water without Se had lower amount of Se in the tissues.
The structure of fish community in the river Mirna
Authors: Jakopin Jasmina , Toman Mihael Jožef
Published: August 2023

Natural perturbations and human pressure increasingly affect the river ecosystems and their biological communities. Fish species are a good indicator of the river status and one of the factors for the assessment of ecological condition by the Water Framework Directive (Directive 2000/60/EC). River Mirna, one of the larger right tributaries of the river Sava, is a good example of anthropogenic pressure on the running water ecosystem. Fish community species structure was analyzed at selected eight sampling sites located over the 28 km length of the river. Abiotic conditions were determined by measuring the physical, chemical and hydro-morphological parameters and the influence of measured parameters on present fish community was analyzed. It has been determined, that the river Mirna is loaded with organic matter and physically changed by regulations, river beds canalizing and bank stabilization. Fish were sampled with standard electro-fishing method to determine species diversity, biomass of the specimens and their size. 29 fish species from seven families (26 native and 3 allochthonous species) were recorded along with one type of the Danubian brook lam- prey (Eudontomyzon vladykovi). Only two species, Telestes souffia and Cottus gobio, were present at all eight sampling sites. The largest number of specimens (890) and the largest biomass (5697 g) per 100 m2 was recorded at the sampling site located after the town as a result of the flow of purified water from the water treatment plant and a large number of invertebrates,which are an important food source. The minimum number of specimens per 100 m2 (86) and the minimum biomass of fish per 100 m2 (457 g) was measured at the relatively natural part of the channel. The most common causes for this are low temperatures, less food and a fast, turbulent flow. The study confirms that the changes in abiotic factors induced by anthropogenic activities significantly influence the structure of fish species community.
Kapusov goseničar (Cotesia glomerata (L.), Hymenoptera, Braconidae) v Sloveniji
Authors: Trdan Stanislav , Bohinc Tanja , Vidrih Matej
Published: August 2023

V prispevku predstavljamo parazitoidno oso kapusovega goseničarja (Cotesia glomerata) in njegov pomen v biotičnem varstvu rastlin. Vrstaje znan naravni sovražnik kapusovega belina (Pieris brassicae), ki je napadom kapusovega goseničarja izpostavljen zlasti v obdobju pojavljanja odprve do tretje larvalne stopnje. Palearktična vrsta, ki je po načinu razvoja endoparazitoid, je bila na območju današnje Slovenije prvič omenjena že leta 1870, vendar podrobnejših zapisov o njenem pojavljanju pri nas ni na voljo. Zastopanost kapusovega goseničarja smo v letu 2014 potrdili na dveh lokacijah v Sloveniji. Namen pričujočega prispevka je predstavitev kapusovega goseničarja z namenom njegove intenzivnejše prihodnje uporabe v varovalnem biotičnem varstvu rastlin.
Cordycepin production by Cordyceps militaris cultivation on spent brewery grains
Authors: Gregori Andrej
Published: August 2023

This is a first report on C. militaris mycelia and fruiting bodies cultivation on solid-state containing spent brewery grains (SBG). Five different strains of C. militaris were cultivated on substrates containing rye grains and 0 to 60% SBG. Stromata formation on SBG-containing substrates was noticed with two C. militaris strains. All strains failed to grow on substrates containing SBG amounts higher than 50%. Highest (10.42 mg/g) cordycepin concentration in cultivating substrate was determined with strain CM2 on 50% SBG. One gram of CM11 strain fungal biomass was able to produce 787.11 mg/g of cordycepin. SBG as a byproduct represent a readily available, low price substrate for cordycepin solid-state production. Obtained concentrations of cordycepin are so far the highest reported concentrations obtained on solid-state substrates therefore we can talk about cordycepin hyperproduction.
Red osier dogwood (Cornus sericea L.) - a new invasive species in Slovenian flora
Authors: Bačič Tinka , Strgulc Krajšek Simona , Jogan Nejc
Published: August 2023

The Red osier dogwood (Cornus sericea L.) is a popular North American ornamental shrub, often planted also in Slovenia. Subspontaneous occurrence of the species in Slovenia is known for two decades, but only in recent years, we can recognize it as an invasive species. The Red osier dogwood is currently known from numerous localities in the wider city area of Ljubljana and also from two sites in the Gorenjska region. It occurs in wetland habitats, particularly in the vicinity of settlements, where the shrub is planted for ornamental purposes. To limit the invasion of the Red osier dogwood, timely measures should be taken. We also publish the updated determination key for the genus Cornus in Slovenia.
In memoriam: profesor dr. Pavel Ličar (21. april 1935-3. oktober 2015)
Authors: Štrus Jasna
Published: August 2023

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Occurrence, toxins and possibilities of control of bloom-forming cyanobacteria of European freshwaters: a review
Authors: Jarni Klara , Griessler Bulc Tjaša , Krivograd Klemenčič Aleksandra
Published: August 2023

Blooming of cyanobacteria is a common problem of eutrophic water bodies in Europe and worldwide and can cause severe problems with toxicity, taste and odour of the water. Toxins produced by cyanobacteria (cyanotoxins) are structurally diverse and their effects range from liver damage, including liver cancer, to neurotoxicity and thus they may present a serious threat for drinking water safety.  Cyanobacterial blooms present major challenges for the management of rivers, lakes and reservoirs and are predicted to cause even worse problems in the future due to the climate change associated with global warming, increased availability of light to phytoplankton and rising levels of atmospheric CO2. This paper presents the literature review of occurrence, toxins (along with their effects on human health) and possibilities of control of bloom-forming cyanobacteria.
The effect of selenium and iodine on selected biochemical and morphological characteristics in kohlrabi sprouts (Brassica oleracea L. var. gongylodes L.)
Authors: Osmić Amela , Golob Aleksandra , Germ Mateja
Published: August 2023

Selenium (Se) and iodine (I) are essential elements for humans and animals, while their essential role for plants has not been established yet. There is also very little information about the interaction between selenium and iodine in plants. The aim of our research was to determine the effect of different forms of Se, I and their combinations on selected biochemical and morphological characteristics of the kohlrabi sprouts (Brassica oleracea L. var. gongylodes L.). Sprouts were grown from seeds, which were soaked in different solutions of selenite, selenate, iodide, iodate and their combinations. We measured the content of chlorophyll a and b, carotenoids, anthocyanins, and UV-A and UV-B absorbing substances. We also measured potential photochemical efficiency of photosystem II (PS II). At the end of the experiment the weight and height of the sprouts were measured. In order to compare the results the entire experiment was carried out twice. Different chemical forms of Se and I, and combinations did not significantly affect the number of sprouts that germinated from seeds. The various chemical forms of Se and I, and combinations differently affected on the amount of pigments in the kohlrabi sprouts. Potential photochemical efficiency of PS II was close to theoretical maximum 0.83.
Variability of testes morphology and the presence of testis-ova in the European blind cave salamander (Proteus anguinus)
Authors: Bizjak Mali Lilijana
Published: August 2023

The European blind cave salamander, Proteus anguinus, is a neotenic, permanently aquatic amphibian with a very long life span, late sexual maturity, and the ability to reproduce for at least 30 years. Proteus is considered to be vulnerable species, and yet very little is known about its reproductive biology. The objective of this study is to describe the detailed morphology of the testes of adult Proteus and determine the maturation state of the gonads and gametogenesis with respect to body size and seasonality. This research showed that testis size increases with adult male body length, but the shape and meiotic condition of the testes are highly variable and independent of the length of the specimen. The testis of Proteus has a simple cystic type of organization in which cysts are enclosed in lobules, with synchronous maturation of the germ cells within each cyst. Spermatogenesis progresses in a caudocephalic direction within the testis, as in other salamanders, and appears to be seasonal, despite the fact that Proteus is a cave animal living in stable environmental conditions. Surprisingly, the testes of approximately one third of the specimens, regardless oftheir morphology or meiotic condition, contained testis-ova located randomly among groups of spermatogonia and spermatocytes. These testes-ova contained germinal vesicles with lampbrush chromosomes, and thus correspond to primary oocytes. Thepresence of testis-ova may be related to a sex-chromosome turnover involving X-Y translocation that was recently discovered in Proteus.
Evaluation of cyanobacteria biomass derived from upgrade of phycocyanin fluorescence estimation
Authors: Rozina Tinkara , Sedmak Bojan , Zupančič Justin Maja , Meglič Andrej
Published: August 2023

The number of harmful cyanobacterial blooms has increased significantly at the global level in recent years. One of the characteristics of cyanobacteria that gives them advantage over other phytoplankton organisms are auxiliary photosyntheticpigments, such as phycocyanin. This fluorescent pigment emits light at a different wavelength as chlorophyll and can therefore be used for detection of cyanobacteria in situ. In this study we used submersible phycocyanin fluorescence sensors and compare their voltage output to concentration of extracted phycocyanin, cell counts and biovolume. The relation was linear in all three cases; however, the variability of regression line slopes between different cyanobacteria strains was high in the case of PC extract concentration and cell count. The highest uniformity in the linear fits was between fluorescence signal and biovolume therefore making it the best candidate for fluorescence sensor voltage output conversion. In the context of this work we also compared different methods for PC extraction. Modifying the equations by subtracting the absorption at 750 nm almost entirely reduces the false PC concentration estimation due to sample turbidity.
Impact of UV radiation and selenium on two buckwheat species
Authors: Golob Aleksandra , Stibilj Vekoslava , Turk Judita , Kreft Ivan , Germ Mateja
Published: August 2023

The impact of selenium (Se) addition and UV radiation on Tartary buckwheat and hybrid buckwheat were studied. Both buckwheat species grew outdoors at the experimental field of the Biotechnical Faculty in Ljubljana. They were exposed to four different treatments regarding the UV radiation (ambient or reduced) and added Se (naturally accessible or foliary treated with Na selenate in concentration 10 mg Se L-1). The content of pigments (chlorophyll a and b, carotenoids, anthocyanins) and UV absorbing compounds, transpiration rate, photochemical efficiency of photosystem II (PS) II and respiratory potential were measured. At the end of experiment we determined the biomass of different plant parts. The results showed that irrespective of the buckwheat species the added Se lowered the content of chlorophyll a and carotenoids, while it increased the effective quantum yield of PS II and transpiration rate. UV radiation reduced the content of anthocyanins only. Se and UV-B radiation as independent factors exerted no impact on buckwheat yield. Hybrid buckwheat had a higher physiological activity than the Tartary buckwheat yet a smaller biomass of plant parts, including reduced yield. Ambient UV radiation had a slightly negative impact on hybrid buckwheat while it had no noticeable negative impact on Tartary buckwheat. The Se treated Tartary and hybrid buckwheat were suitable for human andanimal diet regarding to Se concentrations in leaves and grains.
Z rastlinami povezani že 35 let
Authors: Dermastia Maria
Published: August 2023

V letu 2017 člani Slovenskega društva za biologijo rastlin (http://www.plantslo.org/) praznujemo kar 35 let neprekinjenega delovanja, čeprav ne v enaki zasedbi in tudi ne pod enakim imenom. V tem času se je veliko spremenilo, vendar paje ostala nespremenjena naša strast do odkrivanja skrivnega življenja rastlin.
Family Gammaridae (Crustacea: Amphipoda), mainly its Echinogammarus clade in SW Europe. Further elucidation of its phylogeny and taxonomy
Authors: Sket Boris , Hou Zhonge
Published: August 2023

Most parts of the Echinogammarus clade of Gammaridae have been appropriately classified with the help of a molecular analysis, ultimately freed of the Echinogammarus-Chaetogammarusmalediction. Among defining morphological characters, the gnathopod interrelations are comparatively well congruent with molecular markers. Genus Homoeogammarus distribution area extended from Mediterranean to Atlantic islands. Chaetogammarus and Trichogammarus are firm members of the morphologically very diversified Ponto-Caspian group genera, not closely related to the morphologically similar Echinogammarus, Marinogammarus or Homoeogam-marus. Genus Pectenogammarus (along with Neogammarus and Laurogammarus) synonymized with Homoeogammarus. Parhomoeogammarus diagnose corrected, based on topotype samples of the type species. Freshwater species of the ‘European Eulimnogammarus’ in SW Europe defined asIberogammarus gen. nov. Continental Homoeogammarus-like, but molecularly distinct group, defined as Dinarogammarus gen. nov. Some here accepted genera are molecularly well supported, some are morphologically difficult to distinguish, but each one is morphologically homogeneous; they are also geographically well defined. In both respects, the very speciose and widely spread Homoeogammarus is different.
Vrednotenje velikosti populacij sviščevega mravljiščarja Phengaris alcon (Lepidoptera: Lycaenidae) in status njegove ogroženosti na zahodnem delu Ljubljanskega barja
Authors: Močilar Matija , Verovnik Rudi
Published: August 2023

Na območju zahodnega dela Ljubljanskega barja, smo pregledali vse do sedaj znane lokacije sviščevega mravljiščarja in območja, kjer smo iz satelitskih posnetkov prepoznali potencialno primeren življenjski prostor za to vrsto. Sviščevega mravljiščarja smo potrdili le na območju med Vrhniko in Ligojno. Vrsta se pojavlja na dveh habitatnih krpah, ki sta med seboj oddaljeni 300 metrov. Velikost populacij smo ocenili s pomočjo metode MRR (mark-release-recapture) in s metodo štetja jajčec na hranilnih rastlinah močvirskih sviščev. Kljub majhni oddaljenosti med habitatnima krpama ni prišlo do izmenjave osebkov, smo pa posamezne osebke opazili tudi izven obeh poseljenih krp. Z MRR metodo smo določili dnevno velikost populacije na habitatni krpi A med 50 in 130 osebki, na B pa približno 50 osebkov. Z metodo štetja jajčec, smo ocenili velikost populacije na obeh krpah na približno30 osebkov. Ugotovili smo, da je gostota jajčec in odraslih osebkov na večji habitatni krpi manjša, dnevne velikosti populacij pa so bile večje na večji habitatni krpi. Glavni razlog za tako razporeditev je v pomanjkanju hranilnih rastlin na manjši krpi, saj so bili včasu cvetenja močvirskega svišča vsi sosednji travniki pokošeni. Ugotovili smo tudi, da samice za odlaganje jajčec raje uporabljajo rastline bolj v sredini krpe. Poleg košnje v neprimernem času, sta glavna ogrožajoča dejavnika fragmentiranost življenjskega prostora in hidromelioracijski ukrepi, ki dolgoročno zmanjšujejo nivo podtalnice na tem območju, kar vpliva na habitat vrste.
Effect of ultrasonic algae control devices on non-target organisms: a review
Authors: Klemenčič Pija , Krivograd Klemenčič Aleksandra
Published: August 2023

There is an increasing interest in using ultrasonication in controlling algal (cyanobacterial) blooms and biofouling, a physical method with presumably no adverse effects on non-target organisms, such as fish and zooplankton. At the beginning the use of ultrasound (US) to control algae and biofouling has focused on high-power US causing cavitation; however, due to the potential damage to non-target organisms including marine mammals and human divers, high-power US causing cavitation are not used anymore for algae control in natural environment. Current ultrasonic algae control devices use low-power and thus control algae and biofouling by utilising resonance frequencies and the sound pressure caused by a sound wave propagating through a water column. There are only few studies existing on the effect of US on non-target organisms with incomplete information on wavelengths and intensities of US devices. However, we can conclude that non-cavitation US devices used to control algae and reduce biofouling had no adverse health effects on studied fish species with no feeding and behaviour changes noticed. Caution should be taken when installingUS devices in marine locations since they may interfere with communication between sea mammals or may cause adverse effects on fish from subfamily Alosinae, the only known fish able to detect US. The studies dealing with non-cavitation US used to control algae and biofouling on non-target zooplankton have conflicting results from high mortality to no evident effects. Therefore, caution should be taken when using US for counteract algal growth in ponds or lakes, especially in terms of zooplankton and natural balance maintenance.
UV radiation and temperature effects on functional traits in Helianthemum nummularium subsp. grandiflorum at the alpine and montane site in the Slovenian Alps
Authors: Trošt Sedej Tadeja , Damjanič Rok
Published: August 2023

Alpine plants have evolved strategies to survive harsh conditions, which include high UV and visible radiation, extreme temperatures, dryness and lack of nutrients. Survival strategies include biochemical, physiological and morphological responses, which are scarcely studied because of the time-demanding and complex experimental conditions. We researched functional traits in the alpine plant common rockrose Helianthemum nummularium subsp. grandiflorum growing under ambient UV-B and reduced UV-B radiation at different altitudes (1600 and 2000 m a.s.l.) of mount Vogel in the Slovenian Alps. Leaf anatomy, pigments and optical properties were investigated at the beginning and at the end of the growing season. Plants showed high constitutive UV-absorbing compounds content (UV-AC) throughout the season. Most leaf thickness parameters were not altered according to UV and altitude conditions. Leaves did not transmit any UV spectrum, in agreement with high UV-AC. High photosynthetic spectrum transmittance at alpine altitudes was due to complex biochemical and anatomical responses to these conditions, rather than to UV radiation. Unchanged chlorophyll content of H. nummularium could be related to shrub life form, where leaves shade out high UV and PAR irradiance as well as contribute to lower leaf temperature. This study shows the complexity of alpine plant response, wherespecific characteristics of plant species should not be overlooked.
Stochastic homogenisation of free-discontinuity functionals in randomly perforated domains
Authors: Pellet Xavier , Scardia Lucia , Zeppieri Caterina Ida
Published: August 2023

In this paper, we study the asymptotic behaviour of a family of random free-discontinuity energies

E
ε

{E_{\varepsilon}}

defined in a randomly perforated domain, as ε goes to zero. The functionals

E
ε

{E_{\varepsilon}}

model the energy associated to displacements of porous random materials that can develop cracks. To gain compactness for sequences of displacements with bounded energies, we need to overcome the lack of equi-coerciveness of the functionals. We do so by means of an extension result, under the assumption that the random perforations cannot come too close to one another. The limit energy is then obtained in two steps. As a first step, we apply a general result of stochastic convergence of free-discontinuity functionals to a modified, coercive version of

E
ε

{E_{\varepsilon}}

. Then the effective volume and surface energy densities are identified by means of a careful limit procedure.
Value of brain injury-related indicators based on neural network in the diagnosis of neonatal hypoxic-ischemic encephalopathy
Authors: Wang Lijun
Published: August 2023

Neonatal hypoxic ischemic encephalopathy is a common disease, which is caused by fetal hypoxia, asphyxia, and other reasons. It may cause sequelae of the nervous system and even death in children. Computer tomography examination can clarify the scope and location of the disease and provide the basis for clinical treatment and prognosis. Relevant personnel analyzed the symptoms of ischemic hypoxia and found that ischemia and hypoxia were the main causes of encephalopathy. Neonatal ischemia and hypoxia are easy to cause serious damage. At present, with the development of medicine, the function of the human brain is the most important issue in natural science. The law of neural activity and the role of molecular cells, organs, and systems have fundamental construction significance for the prevention and treatment of nerve and mental diseases. By analyzing the value of the diagnosis of neonatal hypoxic-ischemic encephalopathy in the analysis of experimental data, by setting the newborns in the controlled group and the trial group as experimental subjects, this paper analyzed the situation of newborns in terms of body temperature, body weight, and respiratory rate, and used Apgar score to score these standards. It was found that the score of the controlled group was 7 and above, and the score of the trial group was below 7. It was found that the Apgar scoring method was more simple. Then, the newborns were analyzed by cord blood gas analysis. It was found that most of the data in the control group were between 7.8 and 8.4, and the data in the trial group were between 5.8 and 7.1. The umbilical blood gas analysis score of the experimental group was lower than that of the control group. By comparing the satisfaction of cord blood gas analysis and the Apgar score, it was found that the satisfaction of cord blood gas analysis was 24.06% higher than that of the Apgar score.
Cross-talk between gut microbiota and liver steatosis: Complications and therapeutic target
Authors: Yao Yuan , Shen Yunfeng
Published: August 2023

Liver steatosis is the most widespread chronic liver condition. Its global incidence is rising swiftly and is currently estimated to be 24%. Liver steatosis is strongly related with numerous metabolic syndrome characteristics, like obesity, insulin resistance, hyperlipidemia, and hypertension. The gastrointestinal tract contains about 100 trillion commensal organisms and more than 7,000 distinct bacterial strains. Fat deposition in the liver without secondary causes is known as liver steatosis. Dysregulation of the gut flora is one of the factors connected to the onset of fatty liver disease. Dietary choices may alter constitution of the microbiome and cause gut microbiome dysbiosis, particularly due to the intake of food high in fructose sugars, animal products, and saturated fats. Various gut bacteria cause nutrient metabolism in multiple ways, setting off different inflammatory cascades that encourage liver disease and pathways that help fat build up in the liver. Due to their relatively stable nature, genetic factors may not be responsible for the constant increase in liver steatosis incidence. Genetic factors set the stage for liver steatosis pathogenesis. This review will offer an overview of our present knowledge of the roles played by gut microbiota in regulating the development of liver steatosis, potential side effects, and potential treatment targets.