bluuebunny/crossref_metadata_2025 huggingface dataset:
Preparation and Characterization of Amphoteric Cellulose Hydrogels as Adsorbents for the Anionic Dyes in Aqueous Solutions
Authors: Kono Hiroyuki
Published: August 2015

A series of cellulose ampholytes (CAms), with substitution degrees of cationic groups (DSC) of 0.41, 0.79, and 1.08, and with a substitution degree of anionic groups of 0.68, was prepared from sodium carboxymethylcellulose by a cationization reaction with 2,3-epoxypropyltrimethylammonium chloride. The obtained CAms were crosslinked by ethyleneglycoldiglycidylether (EGDE) to obtain cellulose polyampholyte hydrogels (CAmGs). The CAmGs adsorbed three anionic dyes, viz. AR9, AR13, and AB92, and the absorption depended on the DSC of the hydrogels and the pH of the adsorption medium: the maximum adsorption of anionic dyes occurred using CAmGs with higher DSC values, and under lower pH (less than 3) conditions. The adsorption of these dyes can be fitted by the Langmuir adsorption isotherm model, which revealed the maximum flocculation capacity of CAmGs for each dye. These encouraging results indicate that CAmGs may be applicable for use as novel adsorbents for wastewater treatment.
Detection of Antibiotic Resistant Staphylococcus aureus from Milk: A Public Health Implication
Authors: Akindolire Muyiwa , Babalola Olubukola , Ateba Collins
Published: August 2015

The aim of this study was to investigate the occurrence, antibiotic susceptibility profiles, and virulence genes determinants of S. aureus isolated from milk obtained from retail outlets of the North-West Province, South Africa. To achieve this, 200 samples of raw, bulk and pasteurised milk were obtained randomly from supermarkets, shops and some farms in the North-West Province between May 2012 and April 2013. S. aureus was isolated and positively identified using morphological (Gram staining), biochemical (DNase, catalase, haemolysis and rapid slide agglutination) tests, protein profile analysis (MALDI-TOF mass spectrometry) and molecular (nuc specific PCR) methods. The antimicrobial resistance profiles of the isolates were determined using the phenotypic agar diffusion method. Genes encoding enterotoxins, exfoliative toxins and collagen adhesins were also screened using PCR. Among all the samples examined, 30 of 40 raw milk samples (75%), 25 of 85 bulk milk samples (29%) and 10 of 75 pasteurised milk samples (13%) were positive for S. aureus. One hundred and fifty-six PCR-confirmed S. aureus isolates were obtained from 75 contaminated milk samples. A large proportion (60%–100%) of the isolates was resistant to penicillin G, ampicillin, oxacillin, vancomycin, teicoplanin and erythromycin. On the contrary, low level resistance (8.3%–40%) was observed for gentamicin, kanamycin and sulphamethoxazole. Methicillin resistance was detected in 59% of the multidrug resistant isolates and this was a cause for concern. However, only a small proportion (20.6%) of these isolates possessed PBP2a which codes for Methicillin resistance in S. aureus. In addition, 32.7% of isolates possessed the sec gene whereas the sea, seb sed, see, cna, eta, etb genes were not detected. The findings of this study showed that raw, bulk and pasteurised milk in the North-West Province is contaminated with toxigenic and multi-drug resistant S. aureus strains. There is a need to implement appropriate control measures to reduce contamination as well as the spread of virulent S. aureus strains and the burden of disease in humans.
Investigation of E. coli and Virus Reductions Using Replicate, Bench-Scale Biosand Filter Columns and Two Filter Media
Authors: Elliott Mark , Stauber Christine , DiGiano Francis , De Aceituno Anna , Sobsey Mark
Published: August 2015

The biosand filter (BSF) is an intermittently operated, household-scale slow sand filter for which little data are available on the effect of sand composition on treatment performance. Therefore, bench-scale columns were prepared according to the then-current (2006–2007) guidance on BSF design and run in parallel to conduct two microbial challenge experiments of eight-week duration. Triplicate columns were loaded with Accusand silica or crushed granite to compare virus and E. coli reduction performance. Bench-scale experiments provided confirmation that increased schmutzdecke growth, as indicated by decline in filtration rate, is the primary factor causing increased E. coli reductions of up to 5-log10. However, reductions of challenge viruses improved only modestly with increased schmutzdecke growth. Filter media type (Accusand silica vs. crushed granite) did not influence reduction of E. coli bacteria. The granite media without backwashing yielded superior virus reductions when compared to Accusand. However, for columns in which the granite media was first backwashed (to yield a more consistent distribution of grains and remove the finest size fraction), virus reductions were not significantly greater than in columns with Accusand media. It was postulated that a decline in surface area with backwashing decreased the sites and surface area available for virus sorption and/or biofilm growth and thus decreased the extent of virus reduction. Additionally, backwashing caused preferential flow paths and deviation from plug flow; backwashing is not part of standard BSF field preparation and is not recommended for BSF column studies. Overall, virus reductions were modest and did not meet the 5- or 3-log10 World Health Organization performance targets.
Thyroid Autoimmunity is Associated with Decreased Cytotoxicity T Cells in Women with Repeated Implantation Failure
Authors: Huang Chunyu , Liang Peiyan , Diao Lianghui , Liu Cuicui , Chen Xian , Li Guangui , Chen Cong , Zeng Yong
Published: August 2015

Thyroid autoimmunity (TAI), which is defined as the presence of autoantibodies against thyroid peroxidase (TPO) and/or thyroglobulin (TG), is related to repeated implantation failure (RIF). It is reported that TAI was involved in reproductive failure not only through leading thyroid function abnormality, but it can also be accompanied with immune imbalance. Therefore, this study was designed to investigate the association of thyroid function, immune status and TAI in women with RIF. Blood samples were drawn from 72 women with RIF to evaluate the prevalence of TAI, the thyroid function, the absolute numbers and percentages of lymphocytes. The prevalence of thyroid function abnormality in RIF women with TAI was not significantly different from that in RIF women without TAI (c2 = 0.484, p > 0.05). The absolute number and percentage of T cells, T helper (Th) cells, B cells and natural killer (NK) cells were not significantly different in RIF women with TAI compared to those without TAI (all p > 0.05). The percentage of T cytotoxicity (Tc) cells was significantly decreased in RIF women with TAI compared to those without TAI (p < 0.05). Meanwhile, Th/Tc ratio was significantly increased (p < 0.05). These results indicated that the decreased Tc percentage and increased Th/Tc ratio may be another influential factor of adverse pregnancy outcomes in RIF women with TAI.
Geographic Situational Awareness: Mining Tweets for Disaster Preparedness, Emergency Response, Impact, and Recovery
Authors: Huang Qunying , Xiao Yu
Published: August 2015

Social media data have emerged as a new source for detecting and monitoring disaster events. A number of recent studies have suggested that social media data streams can be used to mine actionable data for emergency response and relief operation. However, no effort has been made to classify social media data into stages of disaster management (mitigation, preparedness, emergency response, and recovery), which has been used as a common reference for disaster researchers and emergency managers for decades to organize information and streamline priorities and activities during the course of a disaster. This paper makes an initial effort in coding social media messages into different themes within different disaster phases during a time-critical crisis by manually examining more than 10,000 tweets generated during a natural disaster and referencing the findings from the relevant literature and official government procedures involving different disaster stages. Moreover, a classifier based on logistic regression is trained and used for automatically mining and classifying the social media messages into various topic categories during various disaster phases. The classification results are necessary and useful for emergency managers to identify the transition between phases of disaster management, the timing of which is usually unknown and varies across disaster events, so that they can take action quickly and efficiently in the impacted communities. Information generated from the classification can also be used by the social science research communities to study various aspects of preparedness, response, impact and recovery.
Variants of MicroRNA Genes: Gender-Specific Associations with Multiple Sclerosis Risk and Severity
Authors: Kiselev Ivan , Bashinskaya Vitalina , Kulakova Olga , Baulina Natalia , Popova Ekaterina , Boyko Alexey , Favorova Olga
Published: August 2015

Multiple sclerosis (MS) is an autoimmune neuro-inflammatory disease arising from complex interactions of genetic, epigenetic, and environmental factors. Variations in genes of some microRNAs—key post-transcriptional regulators of many genes—can influence microRNAs expression/function and contribute to MS via expression changes of protein-coding target mRNA genes. We performed an association study of polymorphous variants of MIR146A rs2910164, MIR196A2 rs11614913, MIR499A rs3746444 MIR223 rs1044165 and their combinations with MS risk and severity. 561 unrelated patients with bout-onset MS and 441 healthy volunteers were enrolled in the study. We observed associations of MS risk with allele MIR223*T and combination (MIR223*T + MIR146A*G/G) carriage in the entire groups and in women at Bonferroni-corrected significance level (pcorr < 0.05). Besides, MIR146A*G/G association with MS was observed in women with nominal significance (pf = 0.025). No MS associations were found in men. A more severe MS course (MSSS value > 3.5) was associated with the carriage of MIR499A*C/T and, less reliably, of MIR499A*C (pcorr = 0.006 and pcorr = 0.024, respectively) and with the carriage of combinations (MIR499A*C/T + MIR196A2*C) and (MIR499A*C + MIR196A2*C) (pcorr = 0.00078 and pcorr = 0.0059, respectively). These associations also showed gender specificity, as they were not significant in men and substantially reinforced in women. The strongest association with MS severity was observed in women for combination (MIR499A*C/T + MIR196A2*C): pcorr = 4.43 × 10−6 and OR = 3.23 (CI: 1.99–5.26).
AGEs-Induced IL-6 Synthesis Precedes RAGE Up-Regulation in HEK 293 Cells: An Alternative Inflammatory Mechanism?
Authors: Serban Andreea , Stanca Loredana , Geicu Ovidiu , Dinischiotu Anca
Published: August 2015

Advanced glycation end products (AGEs) can activate the inflammatory pathways involved in diabetic nephropathy. Understanding these molecular pathways could contribute to therapeutic strategies for diabetes complications. We evaluated the modulation of inflammatory and oxidative markers, as well as the protective mechanisms employed by human embryonic kidney cells (HEK 293) upon exposure to 200 μg/mL bovine serum albumine (BSA) or AGEs–BSA for 12, 24 and 48 h. The mRNA and protein expression levels of AGEs receptor (RAGE) and heat shock proteins (HSPs) 27, 60 and 70, the activity of antioxidant enzymes and the expression levels of eight cytokines were analysed. Cell damage via oxidative mechanisms was evaluated by glutathione and malondialdehyde levels. The data revealed two different time scale responses. First, the up-regulation of interleukin-6 (IL-6), HSP 27 and high catalase activity were detected as early as 12 h after exposure to AGEs–BSA, while the second response, after 24 h, consisted of NF-κB p65, RAGE, HSP 70 and inflammatory cytokine up-regulation, glutathione depletion, malondialdehyde increase and the activation of antioxidant enzymes. IL-6 might be important in the early ignition of inflammatory responses, while the cellular redox imbalance, RAGE activation and NF-κB p65 increased expression further enhance inflammatory signals in HEK 293 cells.
The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii
Authors: Jensen Sara , Neesgaard Vinnie , Skjoldbjerg Sandra , Brandl Martin , Ejsing Christer , Treusch Alexander
Published: August 2015

The functionality of the plasma membrane is essential for all organisms. Adaption to high growth temperatures imposes challenges and Bacteria, Eukarya, and Archaea have developed several mechanisms to cope with these. Hyperthermophilic archaea have earlier been shown to synthesize tetraether membrane lipids with an increased number of cyclopentane moieties at higher growth temperatures. Here we used shotgun lipidomics to study this effect as well as the influence of growth phase on the lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii for the first time. Both species were cultivated at three different temperatures, with samples withdrawn during lag, exponential, and stationary phases. Three abundant tetraether lipid classes and one diether lipid class were monitored. Beside the expected increase in the number of cyclopentane moieties with higher temperature in both archaea, we observed previously unreported changes in the average cyclization of the membrane lipids throughout growth. The average number of cyclopentane moieties showed a significant dip in exponential phase, an observation that might help to resolve the currently debated biosynthesis pathway of tetraether lipids.
Astaxanthin Improves Human Sperm Capacitation by Inducing Lyn Displacement and Activation
Authors: Andrisani Alessandra , Donà Gabriella , Tibaldi Elena , Brunati Anna , Sabbadin Chiara , Armanini Decio , Alvisi Gualtiero , Gizzo Salvatore , Ambrosini Guido , Ragazzi Eugenio , Bordin Luciana
Published: August 2015

Astaxanthin (Asta), a photo-protective red pigment of the carotenoid family, is known for its multiple beneficial properties. In this study, the effects of Asta on isolated human sperm were evaluated. Capacitation involves a series of transformations to let sperm acquire the correct features for potential oocyte fertilization, including the generation of a controlled amount of reactive oxygen species (ROS), cholesterol depletion of the sperm outer membrane, and protein tyrosine phosphorylation (Tyr-P) process in the head region. Volunteers, with normal spermiogram values, were divided in two separate groups on the basis of their ability to generate the correct content of endogenous ROS. Both patient group (PG) and control group (CG) were analysed for Tyr-phosphorylation (Tyr-P) pattern and percentages of acrosome-reacted cells (ARC) and non-viable cells (NVC), in the presence or absence of Asta. In addition, the involvement of ROS on membrane reorganization and the presence of Lyn, a Src family kinase associated with lipid rafts, were investigated. Results show that Lyn is present in the membranes of human sperm, mainly confined in midpiece in resting conditions. Following capacitation, Lyn translocated to the head concomitantly with raft relocation, thus allowing the Tyr-P of head proteins. Asta succeeded to trigger Lyn translocation in PG sperm thus bypassing the impaired ROS-related mechanism for rafts and Lyn translocation. In this study, we showed an interdependence between ROS generation and lipid rafts and Lyn relocation leading the cells to undergo the successive acrosome reaction (AR). Asta, by ameliorating PG sperm functioning, may be utilised to decrease male idiopathic infertility.
RTRO–Coal: Real-Time Resource-Reconciliation and Optimization for Exploitation of Coal Deposits
Authors: Benndorf Jörg , Yueksel Cansin , Shishvan Masoud , Rosenberg Heinrich , Thielemann Thomas , Mittmann Robert , Lohsträter Oliver , Lindig Matthias , Minnecker Corinna , Donner Ralf , Naworyta Wojciech
Published: August 2015

This contribution presents an innovative and integrated framework for real-time-process reconciliation and optimization (RTRO) in large continuous open pit coal mines. RTRO-Coal is currently developed, validated, tested and implemented as part of a multi-national multi-partner European Union funded R&D project. The key concept is to promote a shift in paradigm from intermittent discontinuous to a continuous process monitoring and quality management system in large scale coal mining operations. The framework is based on a real-time feedback control loop linking online data acquired during extraction rapidly with a sequentially up-datable resource model. The up-to-date model is integrated with a real-time optimization of short-term sequencing and production control decisions. Improved decisions are expected to lead to increased resource-and process efficiency and support a sustainable extraction of natural resources. This contribution introduces to the framework, discusses main building blocks and illustrates the value added by the means of selected examples.
Phenyllactic Acid from Lactobacillus plantarum PromotesAdipogenic Activity in 3T3-L1 Adipocyte via Up-Regulationof PPAR-γ2
Authors: Ilavenil Soundharrajan , Kim Da , Valan Arasu Mariadhas , Srigopalram Srisesharam , Sivanesan Ravikumar , Choi Ki
Published: August 2015

Synthetic drugs are commonly used to cure various human ailments at present. However, the uses of synthetic drugs are strictly regulated because of their adverse effects. Thus, naturally occurring molecules may be more suitable for curing disease without unfavorable effects. Therefore, we investigated phenyllactic acid (PLA) from Lactobacillus plantarum with respect to its effects on adipogenic genes and their protein expression in 3T3-L1 pre-adipocytes by qPCR and western blot techniques. PLA enhanced differentiation and lipid accumulation in 3T3-L1 cells at the concentrations of 25, 50, and 100 μM. Maximum differentiation and lipid accumulation were observed at a concentration of 100 μM of PLA, as compared with control adipocytes (p < 0.05). The mRNA and protein expression of PPAR-γ2, C/EBP‑α, adiponectin, fatty acid synthase (FAS), and SREBP-1 were increased by PLA treatment as compared with control adipocytes (p < 0.05). PLA stimulates PPAR-γ mRNA expression in a concentration dependent manner, but this expression was lesser than agonist (2.83 ± 0.014 fold) of PPAR-γ2. Moreover, PLA supplementation enhances glucose uptake in 3T3-L1 pre-adipocytes (11.81 ± 0.17 mM) compared to control adipocytes, but this glucose uptake was lesser than that induced by troglitazone (13.75 ± 0.95 mM) and insulin treatment (15.49 ± 0.20 mM). Hence, we conclude that PLA treatment enhances adipocyte differentiation and glucose uptake via activation of PPAR-γ2, and PLA may thus be the potential candidate for preventing Type 2 Diabetes Mellitus (T2DM).
Accurate Determination of Glacier Surface Velocity Fields with a DEM-Assisted Pixel-Tracking Technique from SAR Imagery
Authors: Yan Shiyong , Liu Guang , Wang Yunjia , Ruan Zhixing
Published: August 2015

We obtained accurate, detailed motion distribution of glaciers in Central Asia by applying digital elevation model (DEM) assisted pixel-tracking method to L-band synthetic aperture radar imagery. The paper firstly introduces and analyzes each component of the offset field briefly, and then describes the method used to efficiently and precisely compensate the topography-related offset caused by the large spatial baseline and rugged terrain with the help of DEM. The results indicate that the rugged topography not only forms the complex shapes of glaciers, but also affects the glacier velocity estimation, especially with large spatial baseline. The maximum velocity, 0.85 m∙d−1, was observed in the middle part on the Fedchenko Glacier, which is the world’s longest mountain glacier. The motion fluctuation on its main trunk is apparently influenced by mass flowing in from tributaries, as well as angles between tributaries and the main stream. The approach presented in this paper was proved to be highly appropriate for monitoring glacier motion and will provide valuable sensitive indicators of current and future climate change for environmental analysis.
A Method for Retrieving Daily Land Surface Albedo from Space at 30-m Resolution
Authors: Gao Bo , Gong Huili , Wang Tianxing
Published: August 2015

Land surface albedo data with high spatio-temporal resolution are increasingly important for scientific studies addressing spatially and/or temporally small-scale phenomena, such as urban heat islands and urban land surface energy balance. Our previous study derived albedo data with 2–4-day and 30-m temporal and spatial resolution that have better spatio-temporal resolution than existing albedo data, but do not completely satisfy the requirements for monitoring high-frequency land surface changes at the small scale. Downscaling technology provides a chance to further improve the albedo data spatio-temporal resolution and accuracy. This paper introduces a method that combines downscaling technology for land surface reflectance with an empirical method of deriving land surface albedo. Firstly, downscaling daily MODIS land surface reflectance data (MOD09GA) from 500 m to 30 m on the basis of HJ-1A/B BRDF data with 2–4-day and 30-m temporal and spatial resolution is performed: this is the key step in the improved method. Subsequently, the daily 30-m land surface albedo data are derived by an empirical method combining prior knowledge of the MODIS BRDF product and the downscaled daily 30-m reflectance. Validation of albedo data obtained using the proposed method shows that the new method has both improved spatio-temporal resolution and good accuracy (a total absolute accuracy of 0.022 and a total root mean squared error at six sites of 0.028).
Temperature Vegetation Dryness Index Estimation of Soil Moisture under Different Tree Species
Authors: Chen Shulin , Wen Zuomin , Jiang Hong , Zhao Qingjian , Zhang Xiuying , Chen Yan
Published: August 2015

The Laoshan forest is the largest forest in Nanjing, and it plays an important role in water resource management in Nanjing. The objectives of this study are to determine if the temperature vegetation dryness index (TVDI) is suitable to estimate the soil moisture and if soil moisture is significantly affected by tree species in the Laoshan forest. This paper calculated the spatial distribution of TVDI using LANDSAT-5 TM data. Sixty-two observation points of in situ soil moisture measurements were selected to validate the effectiveness of the TVDI as an index for assessing soil moisture in the Laoshan forest. With the aid of the three different temporal patterns, which are 10 January 2011, 18 May 2011 and 23 September 2011, this paper used the TVDI to investigate the differences of soil moisture under four kinds of mono-species forests and two kinds of mixed forests. The results showed that there is a strong and significant negative correlation between the TVDI and the in situ measured soil moisture (R2 = 0.15–0.8, SE = 0.015–0.041 cm3/cm3). This means that the TVDI can reflect the soil moisture status under different tree species in the Laoshan forest. The soil moisture under these six types of land cover from low to high is listed in the following order: Eucommia ulmoides, Quercus acutissima, broadleaf mixed forest, Cunninghamia lanceolata, coniferous and broadleaf mixed forest and Pinus massoniana.
The Emergence and Spread of Ecourban Neighbourhoods around the World
Authors: Holden Meg , Li Charling , Molina Ana
Published: August 2015

In modern times, efforts to construct sustainable alternative neighbourhood scale developments date to isolated voluntary initiatives in 1970s Europe and the United States. Since about 2006, they have increased rapidly in popularity. They now go by many names: ecodistricts, écoquartiers, eco-cities, zero/low-carbon/carbon-positive cities, ecopolises, ecobarrios, One Planet Communities, and solar cities. They have become frames—sometimes the dominant frame—used to orient the construction of new pieces of a city in a growing number of countries. Despite numerous standardization efforts, the field of ecourban neighbourhood planning and practice lacks a consistent cross-cultural understanding of what constitutes meaningful ecourbanism in specific economic, political, ecological, social, and design-based terms. Ecourban neighbourhood projects also respond to strictly local challenges and opportunities and express themselves in fragmented ways in different contexts. This article presents an original typology of ecourbanism as the integration of seven extreme type principles. We developed this typology through an abductive approach, or the back and forth testing of observed practices with arguments advanced in theories of sustainable development, planning and urban studies. While ecourban neighbourhood developments by definition express integrative goals, this typology permits assessment of the extent to which outcomes are being achieved in terms of each specific principle. We define and present a limiting case for each of these extreme type principles. Rather than attempting to render different standards equivalent across national contexts, this typology-based approach to understand the outcomes of ecourban neighbourhood developments promises a means to facilitate orienting these developments toward higher levels of integration within a common set of principled boundaries, as they are developed around the world.
Assessing the Environmental Impact of Flax Fibre Reinforced Polymer Composite from a Consequential Life Cycle Assessment Perspective
Authors: Deng Yelin , Tian Yajun
Published: August 2015

The study implements the consequential life cycle assessment (CLCA) to provide a market based perspective on how overall environmental impact will change when shifting glass fibres to flax fibres as reinforcements in composite fabrication. With certain assumptions, the marginal flax fibre supply is identified to be a combination of Chinese flax fibre (70%) and French flax fibre (30%). Due to inferior cultivars and coal-fired electricity in Chinese flax cultivation, the CLCA study reveals that flax mat-PP has 0.8–2 times higher environmental impact values than the glass mat-PP in most environmental impact categories over the production and end-of-life (EoL) phases. For purpose of providing potential trajectories of marginal flax fibre supply, additional scenarios: the “all French fibre”, and “all Chinese fibre” are evaluated formulating the lower and upper boundaries in terms of environmental impact change, respectively. A “the attributional fibre supply mix” scenario is supplied as well. All of these scenarios are useful for policy analysis.
Sustainability of Trade Liberalization and Antidumping: Evidence from Mexico’s Trade Liberalization toward China
Authors: Liu Yi , Zhang Ning
Published: August 2015

A Negative Binomial Regression Model is used to investigate the sustainability of China–Mexico trade liberalization by testing the tariff lines underpinning Mexico’s successful antidumping (AD) measures against Chinese imports from 1991 to 2011. Evidence shows import tariff cutting and consumption growth have a positive impact on consumer goods but a negative impact on intermediaries. This result implies that while the Mexican government has expended considerable energy on the trade liberalization of intermediate and capital goods, the domestic consumer goods market has been protected from Chinese imports. The empirical results indicate that Mexico’s AD use for consumer goods helps to sustain trade liberalization of intermediate and capital goods under the domestic political pressures for trade opening.
Enhancing Organizational Survivability in a Crisis: Perceived Organizational Crisis Responsibility, Stance, and Strategy
Authors: Jeong JiYeon
Published: August 2015

For the purpose of enhancing organizational sustainability during a crisis, an organization takes a position in decision-making, how to respond toward its public, and that is supposed to determine which stance or tactic to employ. This study aims to examine whether publics’ perceptions of organizational crisis responsibility affect their expectations that an organization should choose certain stances and strategies toward the public in a crisis. To address these concerns, an experiment was conducted. As the specific public of this research, health journalists were selected, since they affect public perceptions significantly and public opinion can ultimately put pressure on an organization. Results from an analysis of the experimental data with health journalists confirm that they expect a more accommodative stance/strategy when they perceive that the organization is highly responsible for a health-related crisis. Conversely, when the journalists perceive that an organization has a low level of responsibility for a crisis, they expect a more advocative stance/strategy. By taking into account the health journalists’ expectations along with the needs of the organization, public relations practitioners are better able to make optimal decisions regarding their client organizations’ adopted stance and strategy, and finally, enhance organizational sustainability in a crisis.
Hotel Overbooking and Cooperation with Third-Party Websites
Authors: Dong Yufeng , Ling Liuyi
Published: August 2015

Hotels cooperate with third-party websites to enhance their competitive position and attain sustainable development in the era of e-commerce. Furthermore, hotel managers overbook to hedge against last-minute cancellations and customer no-shows. This paper discusses pricing and overbooking strategies of a hotel in the context of cooperation with multiple third-party websites and analyzes how these strategies influence the cooperation process. The increase in profits resulting from the overbooking strategy is also examined. A model of a Stackelberg game between the hotel and third-party websites and a Nash game among the third-party websites are developed to analyze the process. Results indicate that the compensation coefficient has a slight influence on hotel pricing strategy, but causes a sharp decrease in overbooking level. As the hotel demands to lessen the probability of denying under higher compensation rate, the third-party websites would exert less sales effort to reduce the demand of online customers. Results also show that under a given market demand, hotel effort only serves to redistribute market shares among the hotel and third-party websites, without influencing hotel pricing and overbooking decisions. Specifically, the market shares of the third-party websites decrease in the effort level of the hotel resulting from an increase in the hotel market share. Finally, in the numerical example where one hotel and two websites are analyzed, hotel overbooking strategy improves hotel profit by 4.20%, whereas profit improvements for Websites 1 and 2 are as high as 5.26% and 5.21%, respectively. Managerial implications of the study are also provided.
Ecologizing Our Cities: A Particular, Process-Function View of Southern California, from within Complexity
Authors: Vasishth Ashwani
Published: August 2015

Cities, as the quintessential socio-technological artifacts of human civilization, are seen to set us apart from nature. But an ecosystem view from nested scale-hierarchical process-function ecology shows us that cities are best seen as the emergent and nodal end points of interactive flows of matter, energy and information. From within such a view, a clear need emerges to ecologize our cities by better integrating them back with nature. Arguing from such an ecosystem approach to depicting reality, this paper proposes that tracing the processes and functions which constitute the morphology of the city leads us to articulate an urban ecology that incorporates heat island mitigations, urban forestry, and ecological landscape management (taken both as the introduction of native vegetation and the insertion of increased proportions of pervious paving), all considered within the framework of an integrative ecosystem approach to land use planning. More importantly, such an approach to urban ecology is useful because, as a mode of intervention, it rests on—indeed, requires—an acknowledgement in ecological planning of the often amorphous and usually only indirectly sensible atmospheric, biogeochemical and hydrological processes and functions.
Effects of Melittin Treatment in Cholangitis and Biliary Fibrosis in a Model of Xenobiotic-Induced Cholestasis in Mice
Authors: Kim Kyung-Hyun , Sung Hyun-Jung , Lee Woo-Ram , An Hyun-Jin , Kim Jung-Yeon , Pak Sok , Han Sang-Mi , Park Kwan-Kyu
Published: August 2015

Cholangiopathy is a chronic immune-mediated disease of the liver, which is characterized by cholangitis, ductular reaction and biliary-type hepatic fibrosis. There is no proven medical therapy that changes the course of the disease. In previous studies, melittin was known for attenuation of hepatic injury, inflammation and hepatic fibrosis. This study investigated whether melittin provides inhibition on cholangitis and biliary fibrosis in vivo. Feeding 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) to mice is a well-established animal model to study cholangitis and biliary fibrosis. To investigate the effects of melittin on cholangiopathy, mice were fed with a 0.1% DDC-containing diet with or without melittin treatment for four weeks. Liver morphology, serum markers of liver injury, cholestasis markers for inflammation of liver, the degree of ductular reaction and the degree of liver fibrosis were compared between with or without melittin treatment DDC-fed mice. DDC feeding led to increased serum markers of hepatic injury, ductular reaction, induction of pro-inflammatory cytokines and biliary fibrosis. Interestingly, melittin treatment attenuated hepatic function markers, ductular reaction, the reactive phenotype of cholangiocytes and cholangitis and biliary fibrosis. Our data suggest that melittin treatment can be protective against chronic cholestatic disease in DDC-fed mice. Further studies on the anti-inflammatory capacity of melittin are warranted for targeted therapy in cholangiopathy.
Bio-Control of Salmonella Enteritidis in Foods Using Bacteriophages
Authors: Bao Hongduo , Zhang Pengyu , Zhang Hui , Zhou Yan , Zhang Lili , Wang Ran
Published: August 2015

Two lytic phages, vB_SenM-PA13076 (PA13076) and vB_SenM-PC2184 (PC2184), were isolated from chicken sewage and characterized with host strains Salmonella Enteritidis (SE) ATCC13076 and CVCC2184, respectively. Transmission electron microscopy revealed that they belonged to the family Myoviridae. The lytic abilities of these two phages in liquid culture showed 104 multiplicity of infection (MOI) was the best in inhibiting bacteria, with PC2184 exhibiting more activity than PA13076. The two phages exhibited broad host range within the genus Salmonella. Phage PA13076 and PC2184 had a lytic effect on 222 (71.4%) and 298 (95.8%) of the 311 epidemic Salmonella isolates, respectively. We tested the effectiveness of phage PA13076 and PC2184 as well as a cocktail combination of both in three different foods (chicken breast, pasteurized whole milk and Chinese cabbage) contaminated with SE. Samples were spiked with 1 × 104 CFU individual SE or a mixture of strains (ATCC13076 and CVCC2184), then treated with 1 × 108 PFU individual phage or a two phage cocktail, and incubated at 4 °C or 25 °C for 5 h. In general, the inhibitory effect of phage and phage cocktail was better at 4 °C than that at 25 °C, whereas the opposite result was observed in Chinese cabbage, and phage cocktail was better than either single phage. A significant reduction in bacterial numbers (1.5–4 log CFU/sample, p < 0.05) was observed in all tested foods. The two phages on the three food samples were relatively stable, especially at 4 ºC, with the phages exhibiting the greatest stability in milk. Our research shows that our phages have potential effectiveness as a bio-control agent of Salmonella in foods.
Farmers’ Perceptions about Adaptation Practices to Climate Change and Barriers to Adaptation: A Micro-Level Study in Ghana
Authors: Ndamani Francis , Watanabe Tsunemi
Published: August 2015

This study analyzed the farmer-perceived importance of adaptation practices to climate change and examined the barriers that impede adaptation. Perceptions about causes and effects of long-term changes in climatic variables were also investigated. A total of 100 farmer-households were randomly selected from four communities in the Lawra district of Ghana. Data was collected using semi-structured questionnaires and focus group discussions (FGDs). The results showed that 87% of respondents perceived a decrease in rainfall amount, while 82% perceived an increase in temperature over the past 10 years. The study revealed that adaptation was largely in response to dry spells and droughts (93.2%) rather than floods. About 67% of respondents have adjusted their farming activities in response to climate change. Empirical results of the weighted average index analysis showed that farmers ranked improved crop varieties and irrigation as the most important adaptation measures. It also revealed that farmers lacked the capacity to implement the highly ranked adaptation practices. The problem confrontation index analysis showed that unpredictable weather, high cost of farm inputs, limited access to weather information, and lack of water resources were the most critical barriers to adaptation. This analysis of adaptation practices and constraints at farmer level will help facilitate government policy formulation and implementation.
COMPARISON OF MULTI LAYER PERCEPTRON (MLP) AND RADIAL BASIS FUNCTION (RBF) FOR CONSTRUCTION COST ESTIMATION: THE CASE OF TURKEY
Authors: BAYRAM Savas , OCAL M. Emin , LAPTALI ORAL Emel , ATIS C. Duran
Published: August 2015

In Turkey, for the preliminary construction cost estimation, a notice, which is updated and published annu­ally by Turkish Ministry of the Environment and Urbanism, known as “unit area cost method” (UACM) is generally employed. However, it’s known that the costs obtained through this method in which only construction area is taken into consideration have significant differences from actual costs. The aim of this study is to compare the cost estimations obtained through “multi layer perceptron” (MLP) and “radial basis function” (RBF), which are commonly used artificial neural network (ANN) methods. The results of MLP and RBF were also compared with the results of UACM and the validity of UACM was interpreted. Dataobtained from 232 public construction projects, which completed between 2003 and 2011 in different regions of Turkey, were reviewed. Consequently, estimated costs obtained from RBF were found to be higher than the actual costs with a 0.28% variance, while the estimated costs obtained from MLP were higher than actual values with a 1.11% variance. The approximate costs obtained from UACM are higher than actual costs with a 28.73% variance. It was found that both ANN methods were showed better performance than the UACM but RBF was superior to MLP.
Risk-Based Privacy-Aware Information Disclosure
Authors: Armando Alessandro , Bezzi Michele , Metoui Nadia , Sabetta Antonino
Published: August 2015

Risk-aware access control systems grant or deny access to resources based on the notion of risk. It has many advantages compared to classical approaches, allowing for more flexibility, and ultimately supporting for a better exploitation of data. The authors propose and demonstrate a risk-aware access control framework for information disclosure, which supports run-time risk assessment. In their framework access-control decisions are based on the disclosure-risk associated with a data access request and, differently from existing models, adaptive anonymization operations are used as risk-mitigation method. The inclusion of on-the-fly anonymization allows for extending access to data, still preserving privacy below the maximum tolerable risk. Risk thresholds can be adapted to the trustworthiness of the requester role, so a single access control framework can support multiple data access use cases, ranging from sharing data among a restricted (highly trusted) group to public release (low trust value). The authors have developed a prototype implementation of their framework and have assessed it by running a number of queries against the Adult Data Set from the UCI Machine Learning Repository, a publicly available dataset that is widely used by the research community. The experimental results are encouraging and confirm the feasibility of the proposed approach.
Method Using Command Abstraction Library for Iterative Testing Security of Web Applications
Authors: Munetoh Seiji , Yoshioka Nobukazu
Published: August 2015

A framework based on a scripting language is commonly used in Web application development, and high development efficiency is often achieved by applying several Agile development techniques. However, the adaptation of security assurance techniques to support Agile development is still underway, particularly from the developer's perspective. The authors have addressed this problem by developing an iterative security testing method that splits the security test target application into two parts on the basis of the code lifecycle, application logic (“active development code”) and framework (“used code”). For the former, detailed security testing is conducted using static analysis since it contains code that is changed during the iterative development process. For the latter, an abstraction library at the command granularity level is created and maintained. The library identifies the behavior of an application from the security assurance standpoint. This separation reduces the amount of code to be statically inspected and provides a mechanism for sharing security issues among application developers using the same Web application framework. Evaluation demonstrated that this method can detect various types of Web application vulnerabilities.
PSTPIP2, a Protein Associated with Autoinflammatory Disease, Interacts with Inhibitory Enzymes SHIP1 and Csk
Authors: Drobek Ales , Kralova Jarmila , Skopcova Tereza , Kucova Marketa , Novák Petr , Angelisová Pavla , Otahal Pavel , Alberich-Jorda Meritxell , Brdicka Tomas
Published: August 2015

Mutations in the adaptor protein PSTPIP2 are the cause of the autoinflammatory disease chronic multifocal osteomyelitis in mice. This disease closely resembles the human disorder chronic recurrent multifocal osteomyelitis, characterized by sterile inflammation of the bones and often associated with inflammation in other organs, such as the skin. The most critical process in the disease’s development is the enhanced production of IL-1β. This excessive IL-1β is likely produced by neutrophils. In addition, the increased activity of macrophages, osteoclasts, and megakaryocytes has also been described. However, the molecular mechanism of how PSTPIP2 deficiency results in this phenotype is poorly understood. Part of the PSTPIP2 inhibitory function is mediated by protein tyrosine phosphatases from the proline-, glutamic acid-, serine- and threonine-rich (PEST) family, which are known to interact with the central part of this protein, but other regions of PSTPIP2 not required for PEST-family phosphatase binding were also shown to be indispensable for PSTPIP2 function. In this article, we show that PSTPIP2 binds the inhibitory enzymes Csk and SHIP1. The interaction with SHIP1 is of particular importance because it binds to the critical tyrosine residues at the C terminus of PSTPIP2, which is known to be crucial for its PEST-phosphatase–independent inhibitory effects in different cellular systems. We demonstrate that in neutrophils this region is important for the PSTPIP2-mediated suppression of IL-1β processing and that SHIP1 inhibition results in the enhancement of this processing. We also describe deregulated neutrophil response to multiple activators, including silica, Ab aggregates, and LPS, which is suggestive of a rather generalized hypersensitivity of these cells to various external stimulants.
Novel Noncompetitive IL-1 Receptor–Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth
Authors: Nadeau-Vallée Mathieu , Quiniou Christiane , Palacios Julia , Hou Xin , Erfani Atefeh , Madaan Ankush , Sanchez Mélanie , Leimert Kelycia , Boudreault Amarilys , Duhamel François , Rivera José Carlos , Zhu Tang , Noueihed Baraa , Robertson Sarah A. , Ni Xin , Olson David M. , Lubell William , Girard Sylvie , Chemtob Sylvain
Published: August 2015

Preterm birth (PTB) is firmly linked to inflammation regardless of the presence of infection. Proinflammatory cytokines, including IL-1β, are produced in gestational tissues and can locally upregulate uterine activation proteins. Premature activation of the uterus by inflammation may lead to PTB, and IL-1 has been identified as a key inducer of this condition. However, all currently available IL-1 inhibitors are large molecules that exhibit competitive antagonism properties by inhibiting all IL-1R signaling, including transcription factor NF-κB, which conveys important physiological roles. We hereby demonstrate the efficacy of a small noncompetitive (all-d peptide) IL-1R–biased ligand, termed rytvela (labeled 101.10) in delaying IL-1β–, TLR2-, and TLR4-induced PTB in mice. The 101.10 acts without significant inhibition of NF-κB, and instead selectively inhibits IL-1R downstream stress-associated protein kinases/transcription factor c-jun and Rho GTPase/Rho-associated coiled-coil–containing protein kinase signaling pathways. The 101.10 is effective at decreasing proinflammatory and/or prolabor genes in myometrium tissue and circulating leukocytes in all PTB models independently of NF-κB, undermining NF-κB role in preterm labor. In this work, biased signaling modulation of IL-1R by 101.10 uncovers a novel strategy to prevent PTB without inhibiting NF-κB.
Molecular and cellular mechanisms of muscle aging and sarcopenia and effects of electrical stimulation in seniors
Authors: Barberi Laura , Scicchitano Bianca Maria , Musarò Antonio
Published: August 2015

The prolongation of skeletal muscle strength in aging and neuromuscular disease has been the objective of numerous studies employing a variety of approaches. It is generally accepted that cumulative failure to repair damage related to an overall decrease in anabolic processes is a primary cause of functional impairment in muscle. The functional performance of skeletal muscle tissues declines during post- natal life and it is compromised in different diseases, due to an alteration in muscle fiber composition and an overall decrease in muscle integrity as fibrotic invasions replace functional contractile tissue. Characteristics of skeletal muscle aging and diseases include a conspicuous reduction in myofiber plasticity (due to the progressive loss of muscle mass and in particular of the most powerful fast fibers), alteration in muscle-specific transcriptional mechanisms, and muscle atrophy. An early decrease in protein synthetic rates is followed by a later increase in protein degradation, to affect biochemical, physiological, and morphological parameters of muscle fibers during the aging process. Alterations in regenerative pathways also compromise the functionality of muscle tissues. In this review we will give an overview of the work on molecular and cellular mechanisms of aging and sarcopenia and the effects of electrical stimulation in seniors.