bluuebunny/crossref_metadata_2025 huggingface dataset:
Activation of caspase-3 duringChlamydia trachomatis-induced apoptosis at a late stage
Authors: Matsuo Junji , Haga Sanae , Hashimoto Kent , Okubo Torahiko , Ozawa Takeaki , Ozaki Michitaka , Yamaguchi Hiroyuki
Published: October 2018

The obligate intracellular bacterium Chlamydia trachomatis activates the host cell apoptosis pathway at a late stage of its developmental cycle. However, whether caspase-3, which is a key enzyme of apoptosis, is activated in Chlamydia-infected cells remains unknown. Here, we established HEp-2 cells stably expressing cFluc-DEVD, which is a caspase-3 substrate sequence inserted into cyclic firefly luciferase, and then monitored the dynamics of caspase-3 activity in cells infected with Chlamydia. Transfected cells without infection showed a significant increase in luciferase activity due to stimulation with staurosporine, an inducer of apoptosis. Activation was significantly blocked by addition of caspase inhibitor z-VAD-fmk. Furthermore, as expected, Chlamydia infection caused a significant increase in luciferase activation at 36–48 h postinfection with a contrastive decrease at 24 h postinfection, which is already well known. Such activation caused by the infection was much stronger when the amount of bacteria was increased. Thus, caspase-3 activation was accurately monitored by the luciferase activity in HEp-2 cells constitutively expressing the cFluc-DEVD probe. Furthermore, our data showed that C. trachomatis activates caspase-3 in host cells at a late stage of infection.
The quantum steeplechase
Authors: Das Joy Prakash , Setlur Girish S.
Published: October 2018

Quantum steeplechase is the study of a Luttinger liquid (LL) in one dimension in the presence of a finite number of barriers and wells clustered around an origin. The powerful nonchiral bosonization technique (NCBT) is introduced to write down closed formulas for the two-point functions in the sense of the random phase approximation (RPA). Unlike g-ology-based methods that are tied to the translationally invariant, free particle basis, the NCBT explicitly makes use of the translationally noninvariant single particle wave functions. The present method, that provides the most singular part of the asymptotically exact Green function in a closed form, is in contrast to competing methods that require a combination of renormalization group and/or numerical methods in addition to the bosonization techniques.
Coherent nonlinear low-frequency Landau–Zener tunneling induced by magnetic control of a spin qubit in a quantum wire
Authors: Mkam Tchouobiap S. E. , Danga J. E. , Keumo Tsiaze R. M. , Fai L. C.
Published: October 2018

This paper presents nonlinear Landau–Zener (LZ) tunneling of an electron spin in an accelerating optical parabolic potential, manifested in a heterostructure quantum wire subjected to a periodic magnetic field comprising a spike and a homogeneous part. In this context, driving the two states of a pure nonlinear two-level quantum bit (qubit) system through an avoided level crossing can result in nontrivial dynamics, especially with and without considering a parabolic confinement potential characterized by a curvature confinement potential. We report two striking nonadiabatic and adiabatic scenarios in low modulation frequency limit which appear when such strength modulation occurs. Firstly, the changes of the amplitude of the driving field without considering a parabolic confinement potential act as a perturbation which mixes the spin states. Here, the dynamical evolution of the tunneling probabilities of the nonadiabatic populations under investigation is analyzed. Secondly, for strong fields and strong dependence of a parabolic confinement potential, the two diabatic states do not cross but present anti-crossing phenomenon as the time tends to infinity, describing an adiabatic transition. However, if the field strength in a wire is weak enough, the level separation of a qubit state switches abruptly around the crossing point, and LZ tunneling applies to the whole dynamical range, from adiabatic to fully nonadiabatic crossing. Locally, the tunneling process can be seen as a two-level system (TLS) undergoing a Rabi oscillation. These results open new prospects for the use of quantum interferences in spin–based devices.
DECONSTRUCTING LAWSON AND SAMSON’S CONCEPT OF INNOVATION CAPABILITY: A CRITICAL ASSESSMENT AND A REFINEMENT
Authors: DJOUMESSI ARMAND , CHEN SHU-LING , CAHOON STEPHEN
Published: October 2018

For almost 20 years, research on firm level innovation have relied upon [Lawson and Samson (2001). Developing innovation capability in organisations: A dynamic capabilities approach. International Journal of Innovation Management, 5(3), 377–400] concept of innovation capability (IC). Of note, these authors stated that this concept needs to be ‘refined, validated and tested using other research methods’ [Lawson and Samson (2001). Developing innovation capability in organisations: A dynamic capabilities approach. International Journal of Innovation Management, 5(3), 377–400], p. 396. To date, empirical studies heeding this call have been challenging to find. By researchers relying on this untested concept, they risk not attaining comprehensive insights into the firm level mechanisms underpinning the transformation idea and knowledge into innovations. This paper proposes a rethinking of the IC concept. The analysis is based on survey data of 69 firms involved in the Australian maritime industry using exploratory factor analysis (EFA) and confirmatory factor analysis (CFA). The results suggest that the IC concept might be refined from seven dimensions, initially conceptualised, to three dimensions. The three dimensions are renamed as institutionalising innovation, implementing innovation and stimulating innovation.
Network analysis and GOLAP
Authors: Jin Jennifer , Hayakawa Masahiro
Published: October 2018

Online Analytical Processing (OLAP) is an effective approach to analyzing various complex business problems, and graph is considered as a common scheme to represent the business datasets. Network analysis is a broad analytics scheme for exploring the connectivity and deriving useful analytics results. However, network analysis for graph-based OLAP presents a set of more specific analytics methods by utilizing graph model, network property, and OLAP principles. In this paper, we present a comprehensive survey on network analysis conducted on graph model for the purpose of OLAP, and we summarize the current research focus, paradigms, and the future needs on the target technology.
Mobile Worlds: Choice at the Intersection of Demographic and Environmental Change
Authors: Barnett Jon , Adger W. Neil
Published: October 2018

Research on environmental change has often focused on changes in population as a significant driver of unsustainability and environmental degradation. Demographic pessimism and limited engagement with demographic realities underpin many arguments concerning limits to growth, environmental refugees, and environment-related conflicts. Re-engagement between demographic and environmental sciences has led to greater understanding of the interactions between the size, composition, and distribution of populations and exposure to environmental risks and contributions to environmental burdens. We review the results of this renewed and far more nuanced research frontier, focusing in particular on the way demographic trends affect exposure, sensitivity, and adaptation to environmental change. New research has explained how migration systems interact with environmental challenges in individual decisions and in globally aggregate flows. Here we integrate analysis on demographic and environmental risks that often share a root cause in limited social freedoms and opportunities. We argue for a capabilities approach to promoting sustainable solutions for a more mobile world.
Identification of multiple distinct neurogenic motor patterns that can occur simultaneously in the guinea pig distal colon
Authors: Costa Marcello , Keightley Lauren J. , Wiklendt Lukasz , Hibberd Timothy J. , Arkwright John W. , Omari Taher , Wattchow David A. , Brookes Simon J. H. , Dinning Phil G. , Spencer Nick J.
Published: October 2018

In the guinea pig distal colon, nonpropulsive neurally mediated motor patterns have been observed in different experimental conditions. Isolated segments of guinea pig distal colon were used to investigate these neural mechanisms by simultaneously recording wall motion, intraluminal pressure, and smooth muscle electrical activity in different conditions of constant distension and in response to pharmacological agents. Three distinct neurally dependent motor patterns were identified: transient neural events (TNEs), cyclic motor complexes (CMC), and distal colon migrating motor complexes (DCMMC). These could occur simultaneously and were distinguished by their electrophysiological, mechanical, and pharmacological features. TNEs occurred at irregular intervals of ~3s, with bursts of action potentials at 9 Hz. They propagated orally at 12 cm/s via assemblies of ascending cholinergic interneurons that activated final excitatory and inhibitory motor neurons, apparently without involvement of stretch-sensitive intrinsic primary afferent neurons. CMCs occurred during maintained distension and consisted of clusters of closely spaced TNEs, which fused to cause high-frequency action potential firing at 7 Hz lasting ~10 s. They generated periodic pressure peaks mediated by stretch-sensitive intrinsic primary afferent neurons and by cholinergic interneurons. DCMMCs were generated by ongoing activity in excitatory motor neurons without apparent involvement of stretch-sensitive neurons, cholinergic interneurons, or inhibitory motor neurons. In conclusion, we have identified three distinct motor patterns that can occur concurrently in the isolated guinea pig distal colon. The mechanisms underlying the generation of these neural patterns likely involve recruitment of different populations of enteric neurons with distinct temporal activation properties.
Th1 cytokines TNF-α and IFN-γ promote corticosteroid resistance in developing human airway smooth muscle
Authors: Britt Rodney D. , Thompson Michael A. , Sasse Sarah , Pabelick Christina M. , Gerber Anthony N. , Prakash Y. S.
Published: October 2018

Corticosteroids (CSs) are commonly used to manage wheezing and asthma in pediatric populations. Although corticosteroids are effective in alleviating airway diseases, some children with more moderate-severe asthma phenotypes show CS resistance and exhibit significant airflow obstruction, persistent inflammation, and more frequent exacerbations. Previous studies have demonstrated that Th1 cytokines, such as TNF-α and IFN-γ, promote CS resistance in adult human airway smooth muscle (ASM). In the present study, using a human fetal ASM cell model, we tested the hypothesis that TNF-α/IFN-γ induces CS resistance. In contrast to TNF-α or IFN-γ alone, the combination of TNF-α/IFN-γ blunted the ability of fluticasone propionate (FP) to reduce expression of the chemokines CCL5 and CXCL10 despite expression of key anti-inflammatory glucocorticoid receptor target genes being largely unaffected by TNF-α/IFN-γ. Expression of the NF-κB subunit p65 and phosphorylation of Stat1 were elevated in cells treated with TNF-α/IFN-γ, an effect that remained in the presence of FP. siRNA knockdown studies demonstrated the effects of TNF-α/IFN-γ on increased p65 are mediated by Stat1, a transcription factor activated by IFN-γ. Expression of TNFAIP3, a negative regulator of NF-κB activity, was not altered by TNF-α/IFN-γ. However, the effects of TNF-α/IFN-γ were partially reduced by overexpression of TNFAIP3 but did not influence p65 expression. Together, these data suggest that IFN-γ augments the effects of TNF-α on chemokines by enhancing expression of key inflammatory pathways in the presence of CS. Interactions between TNF-α- and IFN-γ-mediated pathways may promote inflammation in asthmatic children resistant to CSs.
Exercise alters and β-alanine combined with exercise augments histidyl dipeptide levels and scavenges lipid peroxidation products in human skeletal muscle
Authors: Hoetker David , Chung Weiliang , Zhang Deqing , Zhao Jingjing , Schmidtke Virginia K. , Riggs Daniel W. , Derave Wim , Bhatnagar Aruni , Bishop David , Baba Shahid P.
Published: October 2018

Carnosine and anserine are dipeptides synthesized from histidine and β-alanine by carnosine synthase (ATPGD1). These dipeptides, present in high concentration in the skeletal muscle, form conjugates with lipid peroxidation products such as 4-hydroxy trans-2-nonenal (HNE). Although skeletal muscle levels of these dipeptides could be elevated by feeding β-alanine, it is unclear how these dipeptides and their conjugates are affected by exercise training with or without β-alanine supplementation. We recruited 20 physically active men, who were allocated to either β-alanine or placebo-feeding group matched for peak oxygen consumption, lactate threshold, and maximal power. Participants completed 2 wk of a conditioning phase followed by 1 wk of exercise training, a single session of high-intensity interval training (HIIT), followed by 6 wk of HIIT. Analysis of muscle biopsies showed that the levels of carnosine and ATPGD1 expression were increased after CPET and decreased following a single session and 6 wk of HIIT. Expression of ATPGD1 and levels of carnosine were increased upon β-alanine-feeding after CPET, whereas ATPGD1 expression decreased following a single session of HIIT. The expression of fiber type markers myosin heavy chain I and IIa remained unchanged after CPET. Levels of carnosine, anserine, carnosine-HNE, carnosine-propanal, and carnosine-propanol were further increased after 9 wk of β-alanine supplementation and exercise training but remained unchanged in the placebo-fed group. These results suggest that carnosine levels and ATPGD1 expression fluctuates with different phases of training. Enhancing carnosine levels by β-alanine feeding could facilitate the detoxification of lipid peroxidation products in the human skeletal muscle.

NEW & NOTEWORTHY Carnosine synthase expression and carnosine levels are altered in the human skeletal muscle during different phases of training. During high-intensity interval training, β-alanine feeding promotes detoxification of lipid peroxidation products and increases anserine levels in the skeletal muscle.
The combined effect of sprint interval training and postexercise blood flow restriction on critical power, capillary growth, and mitochondrial proteins in trained cyclists
Authors: Mitchell Emma A. , Martin Neil R. W. , Turner Mark C. , Taylor Conor W. , Ferguson Richard A.
Published: October 2018

Sprint interval training (SIT) combined with postexercise blood flow restriction (BFR) is a novel method to increase maximal oxygen uptake (V̇o2max) in trained individuals and also provides a potent acute stimulus for angiogenesis and mitochondrial biogenesis. The efficacy to enhance endurance performance, however, has yet to be demonstrated. Trained male cyclists ( n = 21) (V̇o2max: 62.8 ± 3.7 ml·min−1·kg−1) undertook 4 wk of SIT (repeated 30-s maximal sprints) either alone (CON; n = 10) or with postexercise BFR ( n = 11). Before and after training V̇o2max, critical power (CP) and curvature constant ( Wʹ) were determined and muscle biopsies obtained for determination of skeletal muscle capillarity and mitochondrial protein content. CP increased ( P = 0.001) by a similar extent following CON (287 ± 39 W to 297 ± 43 W) and BFR (296 ± 40 W to 306 ± 36 W). V̇o2max increased following BFR by 5.9% ( P = 0.02) but was unchanged after CON ( P = 0.56). All markers of skeletal muscle capillarity and mitochondrial protein content were unchanged following either training intervention. In conclusion, 4 wk of SIT increased CP; however, this was not enhanced further with BFR. SIT was not sufficient to elicit changes in skeletal muscle capillarity and mitochondrial protein content with or without BFR. However, we further demonstrate the potency of combining BFR with SIT to enhance V̇o2max in trained individuals.

NEW & NOTEWORTHY This investigation has demonstrated that 4 wk of sprint interval training (SIT) increased critical power in trained individuals; however, postexercise blood flow restriction (BFR) did not enhance this further. SIT, with or without BFR, did not induce any changes in skeletal muscle capillarity or mitochondrial protein content in our trained population. We do, however, confirm previous findings that SIT combined with BFR is a potent stimulus to enhance maximal oxygen uptake.
Neurotrophic Activity of the Carrageenophyte Kappaphycus alvarezii Cultivated at Different Depths and for Different Growth Periods in Various Areas of Indonesia
Authors: Tirtawijaya Gabriel , Meinita Maria Dyah Nur , Marhaeni Bintang , Haque Md. Nazmul , Moon Il Soo , Hong Yong-Ki
Published: October 2018

The carrageenophyte Kappaphycus alvarezii (Rhodophyta) has neurotrophic activity in primary hippocampal neurons. This seaweed is abundant and easily cultivated in tropical coastal areas. To determine the best growth conditions for neurotrophic activity, thalli were grown at different depths and for different periods in various areas of Indonesia. Neurotrophic activity was measured based on the number of primary neurites, the total length of the primary neurites, and the length of the longest neurite. K. alvarezii had higher neurotrophic activity than carrageenophytes K. striatum and Eucheuma denticulatum cultured under the same conditions. K. alvarezii grown at the surface for 45 days had higher (1.4‐ to 1.8‐fold) neurotrophic activity than thalli grown at depth (2 m) or harvested sooner (15 days) (P < 0.05). Relatively high activities were detected in thalli cultured at Ternate and Garut, Indonesia. Therefore, from a commercial perspective, the culture conditions at the surface for 45 days were optimal for the production of both neurotrophic compounds and carrageenan. K. alvarezii produced neurotrophic compounds under various environmental conditions, although some conditions were optimal.
Influence of Macrotexture and Microtexture on the Skid Resistance of Aggregates
Authors: Guan Bowen , Wu Jiayu , Xie Chao , Fang Jianhong , Zheng Haile , Chen Huaxin
Published: October 2018

This article intends to study the influence of macrotexture and microtexture on the skid resistance of four types of aggregates. For this purpose, fractal dimension (D), root mean square height (Rq), and Polished Stone Value (PSV) were tested. The Pearson correlation coefficients between PSV and D or Rq in the interval of different polishing cycles were calculated and analyzed with correlation analysis. The relationships between PSV and Rq were also established. The results showed that the PSV development was approximately divided into 3 stages including accelerated attenuation stage, decelerated attenuation stage, and stabilization stage. There is a critical point of the entire polishing cycles. When the number of the polishing cycles exceeds this critical point, microtexture replaces macrotexture to play a major role in the skid resistance of aggregates. In the accelerated attenuation stage, macrotexture plays a major role in the skid resistance of aggregates. In the decelerated attenuation stage and stabilization stage, microtexture gradually plays a major role in the skid resistance of aggregates. Because of roughest microtexture in the stabilization stage, bauxite can provide the highest levels of skid resistance for high friction surface treatment over the long‐term period.
Grain Amaranth Is Associated with Improved Hepatic and Renal Calcium Metabolism in Type 2 Diabetes Mellitus of Male Wistar Rats
Authors: Kasozi Keneth Iceland , Namubiru Sarah , Safiriyu Abass Alao , Ninsiima Herbert Izo , Nakimbugwe Dorothy , Namayanja Monica , Valladares Miriela Betancourt
Published: October 2018

Background. Dysregulation of calcium signaling is a hallmark of diabetes mellitus (DM) and grain amaranth (AG) has antidiabetic properties. Information on the mechanism of action of AG on blood, renal, and hepatic tissues is sparse, although it continues to be an important alternative medicinal plant in several developing countries. The objective of the study was to determine key changes in calcium levels and s100a1 protein levels and antioxidant and histopathologic changes in blood, renal, and hepatic tissues of male diabetic Wistar rats. Materials and Methods. This was an experimental study in which 30 male Wistar rats were kept for 5 weeks (6 groups, N =5). Groups 1‐IV had T2DM induced using Nicotinamide and Streptozotocin: Group I, Mixtard®; group II, positive control; group III, 25% AG; group IV, 50% AG. Furthermore, group V consisted of normal rats given 50% GA and group VI was negative control. Blood, renal, and hepatic tissues were collected and analyzed for calcium, s100a1 protein levels, and antioxidant and histopathological changes. Results and Discussion. In blood, renal, and hepatic tissue, calcium and s100a1 levels were low during T2DM and these increased following AG supplementation. This was important for improved metabolic processes, thus leading to the low malondialdehyde (MDA) and glutathione peroxidase (GPx) activity in the tissues. Efficient antioxidant status was important for improved calcium signaling mechanisms, thus leading to improved tissue function and protection demonstrating the importance of AG as an alternative medicinal source through the calcium signaling pathway. Conclusion. Grain amaranth exerts its antidiabetic properties through improved calcium homeostasis in blood, kidney, and liver.
A Rare Case of Complete Fragmentation of Pacemaker Lead after a High-Velocity Theme Park Ride
Authors: Khattak Furqan , Khalid Muhammad , Gaddam Sathvika , Ramu Vijay , Brahmbhatt Vipul
Published: October 2018

Pacemaker lead fracture is one of the most common causes of pacemaker malfunction and is most frequently associated with weight lifting or chest trauma. These patients usually present with symptoms of dizziness, syncope, chest discomfort, and palpitations or less commonly with extracardiac symptoms. Diagnosis is made by ECG and careful review of chest imaging such as chest X-ray or fluoroscopy. Treatment involves placement of a new lead with or without extraction of the fractured lead. We present an interesting case of complete severance of the tip of a dual-chamber pacemaker’s atrial lead after a high-velocity theme park ride. In our case, the fracture occurred during amusement park rides and went undiagnosed until the patient presented for routine pacemaker evaluation. This case indicates that extreme physical forces in the absence of direct trauma, such as during amusement park rides, may result in lead fractures and patients with pacemakers should be cautioned regarding such activities.
Supply Chain Cost Prediction for Prefabricated Building Construction under Uncertainty
Authors: Wang Shaoli , Mursalin Yasir , Lin Gang , Lin Conghua
Published: October 2018

This paper considers the problem of predicting a supply chain cost for prefabricated building construction under an uncertain situation. Based on the Activity-Based Costing (ABC) method, we establish a computational model to estimate the total cost of the prefabricated supply chain. The model can assist in not only finding the critical areas for cost reduction of the whole supply chain but also analyzing other costs of prefabricated construction projects. Furthermore, we provide a numerical example of prefabricated building construction to validate the results.
Clinical and Mutation Analysis of Patients with Best Vitelliform Macular Dystrophy or Autosomal Recessive Bestrophinopathy in Chinese Population
Authors: Gao Tingting , Tian Chengqiang , Hu Qinrui , Liu Zhiming , Zou Jimei , Huang Lvzhen , Zhao Mingwei
Published: October 2018

Mutations in the gene BEST1 usually cause bestrophinopathies, such as the rare progressive diseases Best vitelliform macular dystrophy (BVMD) and autosomal recessive bestrophinopathy (ARB). This study aimed to investigate the clinical characteristics of patients with BVMD or ARB carrying BEST1 mutations. A total of 12 probands including 9 patients with a clinical diagnosis of BVMD and 3 patients with a clinical diagnosis of ARB were recruited for genetics analysis. All patients underwent detailed ophthalmic examination. All coding exons of the BEST1 gene were screened by PCR-based DNA sequencing. Programs of PolyPhen-2, SIFT, and MutationTaster were used to analyze the potential pathogenicity of the mutations in BEST1. In the 9 unrelated patients with BVMD, one heterozygous BEST1 mutation was revealed in 8 patients and two compound heterozygous mutations in 1 patient. In the 3 unrelated patients with ARB, two compound heterozygous mutations were revealed in 2 patients and three compound heterozygous mutations in 1 patient. Molecular analyses identified a total of 15 mutations, including 3 novel mutations (c.424A>G p.S142G, c.436G>A p.A146T, and c.155T>C p.L52P). Antivascular endothelial growth factor (VEGF) drugs were given to two affected eyes, especially those also exhibiting choroidal neovascularization (CNV), and no serious adverse events occurred. Our study indicates that there is wide genotypic and phenotypic variability in patients with BVMD or ARB in China. The screening of BEST1 gene is significant for the precise diagnosis of BVMD and ARB.
How to Modulate Tumor Hypoxia for Preclinical In Vivo Imaging Research
Authors: De Bruycker Sven , Vangestel Christel , Staelens Steven , Van den Wyngaert Tim , Stroobants Sigrid
Published: October 2018

Tumor hypoxia is related with tumor aggressiveness, chemo- and radiotherapy resistance, and thus a poor clinical outcome. Therefore, over the past decades, every effort has been made to develop strategies to battle the negative prognostic influence of tumor hypoxia. For appropriate patient selection and follow-up, noninvasive imaging biomarkers such as positron emission tomography (PET) radiolabeled ligands are unprecedentedly needed. Importantly, before being able to implement these new therapies and potential biomarkers into the clinical setting, preclinical in vivo validation in adequate animal models is indispensable. In this review, we provide an overview of the different attempts that have been made to create differential hypoxic in vivo cancer models with a particular focus on their applicability in PET imaging studies.
What Are the Participants’ Perspectives of Taking Melatonin for the Treatment of Nocturia in Multiple Sclerosis? A Qualitative Study Embedded within a Double-Blind RCT
Authors: Khan Rafiyah , Uren Alan , Canham Luke , Cottrell David , Drake Marcus J. , Cotterill Nikki
Published: October 2018

Background. Multiple Sclerosis (MS) is a chronic neurological disorder caused by neurodegeneration within the central nervous system. It results in impaired physical, cognitive, and psychological functioning and can also lead to lower urinary tract symptoms including nocturia. While clinical trials have suggested an association between nocturia and melatonin secretion, to our knowledge, no qualitative research has been conducted on the experience of taking melatonin to treat nocturia in progressive MS within a clinical trial. Methods. 17 semistructured qualitative interviews were conducted as part of a double-blind, randomised, placebo controlled, crossover, clinical trial with consenting adults with MS. Interviews explored participants’ experiences of nocturia associated with MS and their experience of taking melatonin as a trial treatment for nocturia versus a placebo. Data was analysed using a thematic analysis. Results. Themes on the experience of nocturia revealed participants’ understandings of nocturia, the impact it had on their night, and increased daily fatigue. Themes on the intervention showed perceived improvements to nocturia, sleep, and energy and negative effects including lethargy, a lack of significant change, and physical side effects including vivid dreams. Conclusion. This qualitative exploration revealed an association between nocturia and increased levels of fatigue during the day by those with MS. However, perspectives towards the effectiveness of melatonin as a potential treatment varied as both placebo and melatonin were perceived as having very similar effects.
Effects of Two Types of 9-Month Adapted Physical Activity Program on Muscle Mass, Muscle Strength, and Balance in Moderate Sarcopenic Older Women
Authors: Piastra G. , Perasso L. , Lucarini S. , Monacelli F. , Bisio A. , Ferrando V. , Gallamini M. , Faelli E. , Ruggeri P.
Published: October 2018

The present study aimed to evaluate the effects of two types of 9-month adapted physical activity (APA) program, based on a muscle reinforcement training and a postural training, respectively, on muscle mass, muscle strength, and static balance in moderate sarcopenic older women. The diagnosis of sarcopenia was done in accordance with measurable variables and cut-off points suggested by the European Working Group on Sarcopenia in Older People (EWGSOP). Seventy-two participants were randomly assigned to two groups: the muscle reinforcement training group (RESISTANCE) (n=35; 69.9 ± 2.7 years) and the postural training group (POSTURAL) (n=37; 70.0±2.8 years). Body composition, muscle mass, skeletal muscle mass index (SMI), and handgrip strength (HGS) were evaluated for sarcopenia assessment, whereas Sway Path, Sway Area, Stay Time, and Spatial Distance were evaluated for static balance assessment. Sixty-six participants completed the study (RESISTANCE group: n=33; POSTURAL group: n=33). Significant increases of muscle mass, SMI, and handgrip strength values were found in the RESISTANCE group, after muscle reinforcement program. No significant differences appeared in the POSTURAL group, after postural training. Furthermore, RESISTANCE group showed significant improvements in static balance parameters, whereas no significant differences appeared in the POSTURAL group. On the whole, the results of this study suggest that the APA program based on muscle reinforcement applied on moderate sarcopenic older women was able to significantly improve muscle mass and muscle strength, and it was also more effective than the applied postural protocol in determining positive effects on static balance.
Bacterial and Parasitic Assessment from Fingernails in Debre Markos, Northwest Ethiopia
Authors: Mengist Abeba , Aschale Yibeltal , Reta Alemayehu
Published: October 2018

Background. Food handlers with untrimmed finger nails could contribute or serve as a vehicle for the transmission of food poisoning pathogens. Objectives. This study was conducted to determine the prevalence of bacteria and intestinal parasites among food handlers and antibiotic susceptibility profile of the isolated bacteria in Debre Markos University, Ethiopia. Materials and Methods. This laboratory-based cross-sectional study involved 220 food handlers working in food service establishments in Debre Markos University between 1st January 2015 to 31th June 2016. Subjects’ finger nail specimens of both hands were examined microscopically for intestinal parasites. For bacterial isolation, samples were cultured and bacterial species were identified following standard laboratory procedures. Antimicrobial susceptibility test was performed for all bacterial isolates by using Kirby-Bauer disk diffusion method. Results. Of the total 220 subjects examined, 29.5% showed positive culture for different bacterial species from their fingernail contents. Coagulase-negative Staphylococcus was the predominant bacteria species (12.3%) followed by Staphylococcus aureus (5%), E. coli (2.7%), Klebsiella species (2.7%), Enterococcus species (1.8%), Pseudomonas aeruginosa (1.8%), Proteus species (1.4%), Citrobacter species (1.4%), and Serratia species (0.9%). None of the food handlers showed positive culture for Shigella and salmonella and parasites in respect of their finger nail specimens. Isolation of bacteria in finger nail has significant association with finger nail status (P=0.044) and inverse relation with service years (P=0.048). All Staphylococcus aureus and coagulase-negative Staphylococcus species isolates were uniformly susceptible to vancomycin. Only one (9.1%) of Staphylococcus aureus isolates was resistant for methicillin. Conclusion. To prevent the food poisoning pathogens, implementation and adherence to infection are the key practices, specially food handlers with long finger nail harbor food debris, microbial contaminations, and allergens.
Electroacupuncture Relieves Visceral Hypersensitivity by Inactivating Protease‐Activated Receptor 2 in a Rat Model of Postinfectious Irritable Bowel Syndrome
Authors: Xu Wanli , Yuan Mengqian , Wu Xiaoliang , Geng Hao , Chen Lu , Zhou Junling , Song Yafang , Pei Lixia , Sun Jianhua
Published: October 2018

Background. The role of protease‐activated receptor 2 (PAR2) in the analgesic effect of electroacupuncture (EA) on visceral hypersensitivity (VH) in postinfectious irritable bowel syndrome (PI‐IBS) has yet to be elucidated. Aim. In this study, we investigated the molecular mechanisms underlying the analgesic effect of EA in a rat model of PI‐IBS. Methods. Visceral hypersensitivity was evaluated by the abdominal withdrawal reflex test before and after administration of the PAR2 agonist, PAR2‐AP, and/or EA. The protein expression and mRNA levels of PAR2, CGRP, SP, and TPSP in colon tissues were measured by immunofluorescence, western blot, and RT‐PCR. Results. We found that EA could alleviate VH and significantly decrease protein and mRNA levels of PAR2, TPSP, CGRP, and SP in PI‐IBS rats. The analgesic effect of EA on VH was slightly reduced in the presence of PAR2‐AP. Conclusions. These results suggest that EA alleviates VH symptoms through downregulation of the levels of the TPSP/PAR2/SP/CGRP signaling axis in colon tissues in PI‐IBS rats. Together, our data suggests that PAR2 plays a critical role in the analgesic effect of EA on VH in PI‐IBS.
Behavior of Smooth Muscle Cells under Hypoxic Conditions: Possible Implications on the Varicose Vein Endothelium
Authors: Ortega Miguel A. , Romero Beatriz , Asúnsolo Ángel , Sainz Felipe , Martinez-Vivero Clara , Álvarez-Mon Melchor , Buján Julia , García-Honduvilla Natalio
Published: October 2018

Varicose veins are a disease with high incidence and prevalence. In the venous wall, the smooth muscle cells (SMCs) act in the vascular homeostasis that secretes multiple substances in response to stimuli. Any alteration of these cells can modify the function and structure of the other venous layers such as the endothelium, resulting in increases in endothelial permeability and release of substances. Therefore, knowing the cellular and molecular mechanisms of varicose veins is imperative. The aims of this study are to understand how SMCs of patients with varicose veins subjected to saphenectomy of the great saphenous vein react under hypoxic cell conditions and to determine the role of vascular endothelial growth factor (VEGF) in this process. We obtained SMCs from human saphenous vein segments from patients with varicose veins (n=10) and from organ donors (n=6) undergoing surgery. Once expanded, the cells were subjected to hypoxic conditions in specific chambers, and expansion was examined through analyzing morphology and the expression of α-actin. Further gene expression studies of HIF-1α, EGLN3, VEGF, TGF-β1, eNOS, and Tie-2 were performed using RT-qPCR. This study reveals the reaction of venous cells to sustained hypoxia. As significant differential gene expression was observed, we were able to determine how venous cells are sensitive to hypoxia. We hypothesize that venous insufficiency leads to cellular hypoxia with homeostatic imbalance. VEGF plays a differential role that can be related to the cellular quiescence markers in varicose veins, which are possible therapeutic targets. Our results show how SMCs are sensitive to hypoxia with a different gene expression. Therefore, we can assume that the condition of venous insufficiency leads to a situation of sustained cellular hypoxia. This situation may explain the cellular response that occurs in the venous wall as a compensatory mechanism.
Screening for Depressive Symptoms among Patients Attending Specialist Medical Outpatient Clinics in a Tertiary Hospital in Southern Nigeria
Authors: Ofori Sandra N. , Adiukwu Frances N.
Published: October 2018

This study aimed to determine the prevalence of unrecognized depressive symptoms and its associated risk factors among patients with diabetes and/or hypertension attending medical outpatient clinics of a tertiary health centre in southern Nigeria. A cross-sectional study design was employed to assess 200 randomly selected patients attending the clinics. Questionnaires were administered to obtain sociodemographic and medical history data. The perceived stress scale (PSS) was used to determine the presence of subjective psychological stress and PHQ-9 was used to screen for depression. The prevalence of depressive symptoms was 54.9% with 16.5% categorised as having major depression. After adjusting for confounding variables, age younger than 60 years was associated with less odds of having depressive symptoms (AOR 0.32, 95% CI 0.17, 0.62; p=0.001), while only significant psychological stress increased the odds of having depressive symptoms (AOR 2.78, 95% CI 1.37, 5.64; p=0.005). The prevalence of depression among the study participants is high and has the potential to significantly impact the control of their disease and ultimately contribute to the high cardiovascular risk faced by this population.
Storage and Flux of Nutrients in Disturbed and Undisturbed Tropical Moist Forest of Eastern Nepal
Authors: Gautam Tilak Prasad , Mandal Tej Narayan
Published: October 2018

The disturbance activities in tropical forests shrink the nutrient cycling between the vegetation and soil. To understand the nutrient cycling in undisturbed and disturbed stands of mixed deciduous tropical forest of eastern Nepal, plant biomass was estimated within seventy randomly established sampling plots. The biomass values were multiplied with nutrient concentration of respective parts to estimate the nutrient stocks. The nutrient concentrations varied widely amongst components. In trees, concentrations of all nutrients were highest in leaves followed in decreasing order by fine roots (<5 mm) and twigs and then by branches, bole, and coarse roots. The contribution of different components to total nutrient stocks was in the following order: tree > stand fine root > shrub > herb, in both stands. The relative contribution of different components of trees to total nutrient stocks was in the following order: bole > coarse root > branch > leaf > twig > fine roots. In trees, leaves and fine roots had greater gross uptake of nutrients than other components. The concentrations of different nutrients in the plants are in the following order: nitrogen > potassium > phosphorus. Total nutrient return to the soil through the litterfall is almost 1.5 times greater than that from fine roots in both forests. In conclusion, various types of forest disturbances had adverse effect on the nutrient stocks and nutrient dynamics.
Preparation of Magnetic Nanoliposomes of Sesquiterpene‐Rich Fraction from Cichorium glandulosum and Its Tissue Distribution in Mice
Authors: Luo Yuqin , Yang Weijun , Abuduaini Abudujilili , Aisa Haji Akber
Published: October 2018

Objective. To prepare and study the distribution of magnetic nanoliposomes of Sesquiterpene‐Rich Fraction from Cichorium glandulosum (SRF‐MLN) (under magnetic field) in the main organs of mice. Methods. The SRF‐MLN was prepared by ethanol injection‐sonication precipitation method. The quality of its pharmaceutical properties was investigated by the active ingredient lactucin. The drug concentration and distribution of lactucin in different tissues and organs including whole blood, liver, heart, spleen, lung, and kidney were evaluated with Sesquiterpene‐Rich Fraction from Cichorium glandulosum (SRF‐LP) as a control. Results. The prepared SRF‐MLN was spherical and monodispersed with an average particle diameter of 65 ± 1 nm, encapsulation efficiency of 91.3%  ± 1.5% (RSD% = 1.5), and drug loading rate of 4.7%  ± 0.3% (RSD% = 0.7). Dispersion coefficient PDI was 0.157 and Zeta potential was −17.5 mV. The lactucin concentration of SRF‐MLN after 10 min of intravenous injection in heart, liver, spleen, lung, and kidney was significantly higher than that of SRF‐LP group. The AUC0-12h values of liver tissue were obviously higher than other tissues and MRT was significantly prolonged under the action of the magnetic field (p<0.01). Conclusion. The prepared SRF‐MLN can change the distribution of drugs in different tissues and organs, prolonging the action time of the drugs in the body, and it has certain specificity under the action of applied magnetic field.
Introducing Videoconferencing on Tablet Computers in Nurse–Patient Communication: Technical and Training Challenges
Authors: Rygg Lisbeth O. , Brataas Hildfrid V. , Nordtug Bente
Published: October 2018

Background. This article examines personnel and patient experiences of videoconferencing (VC) trials on tablet computers between oncology certified nurses (OCNs) and patients with cancer who live at home. The study points to organizational pitfalls during the introduction process. In many different arenas, the use of VC has increased recently owing to improved Internet access and capacity. This creates new opportunities for contact between patients living at home and their nurses. Video conferencing presupposes knowledge about Internet access, training, and usability of technological equipment. The aim of this pilot study was to illuminate patients’ and nurses’ experiences of the technical functionality, usability, and training of tablet use in VC in primary cancer care. The results point to the drawbacks concerning the introduction of VC. Method. A pilot study with an explorative design was used to describe patients’ and OCNs’ experiences of technical functionality and usability of VC on tablet computers. After a three-month trial, data were gathered, focusing on both patients’ and nurses’ perspectives. Individual interviews with four female OCNs, aged 32–65 (mean 46), and six patients with cancer, two men and four women aged 49–78 (mean 69), were content-analyzed. Results. The analysis revealed two main categories: network connectivity and tablet usability and training and educational pitfalls. Conclusion. When planning VC implementation, the organizational leadership should consider network access and stability, as well as individualized VC training on tablets. Ensuring patient safety should also be a priority. Further research should provide knowledge of technological and educational pitfalls, and possible implications of VC on the care quality of nursing.
Percutaneous Total Endoscopic Resection of Partial Articular Processes for Treatment of Lateral Crypt Stenosis and Lumbar Spinal Stenosis: Technical Report and Efficacy Analysis
Authors: Wu Fujun , Kong Weijun , Liao Wenbo , Ao Jun , Ye Sheng , Du Qian , Wang Ansu
Published: October 2018

Objective. To observe the clinical curative effect of posterior total endoscopic precision decompression for the treatment of single-segment lateral crypt lumbar spinal stenosis (LSS). Method. A total of 27 patients with single-segment LSS satisfying the inclusion criteria were recruited from July 2013 to September 2015. There were 18 cases of unilateral stenosis of the L4-5 segments and 9 cases of unilateral stenosis of the L5-S1 segment. All patients were treated via the posterior approach with the precise lateral crypt decompression technique. Precise decompression was performed on the narrow areas causing clinical symptoms. Clinical efficacy was assessed at 3 days, 3 months, 6 months, and 2 years after surgery. Low-back pain and sciatic nerve pain assessed by visual analog scale (VAS) score and the functional Oswestry Disability Index (ODI) were used to evaluate lumbar function, and modified MacNab score criteria were used to investigate long-term efficacy. Result. All patients completed the operation successfully, and the follow-up time was 2 years. The VAS score of lumbago was lower after than before surgery (preoperative: 6.96±0.90; postoperative: 2.04±1.02, P<0.05). The VAS score of sciatica was also lower after than before surgery (preoperative: 7.19±0.88, postoperative: 1.93±0.92, P<0.05), and the ODI was improved at the last follow-up (29.62±4.26) % compared with before surgery (80.07±3.98) %. The MacNab efficacy evaluation showed improvement at the end of the follow-up period: 20 cases were excellent, 6 cases were good, and 1 case was satisfactory, with a good/excellent rate of 96%. No surgical site infections, iatrogenic nerve root injuries, epidural hematomas, or other complications occurred. Conclusion. Total endoscopic decompression of posterior facet arthrodesis for the treatment of single-segment lateral crypt LSS has the advantages of safety, reduced recurrence and trauma, and a satisfactory curative effect. This trial is registered with ChiCTR1800015628.
C-Reactive Protein and Platelet-Lymphocyte Ratio as Potential Tumor Markers in Low-Alpha-Fetoprotein Hepatocellular Carcinoma
Authors: Suner Aslı , Carr Brian I. , Akkiz Hikmet , Karakülah Gökhan , Üsküdar Oguz , Yalçın Kendal , Kuran Sedef , Tokat Yaman , Yilmaz Sezai , Özakyol Ayşegül , Tokmak Salih , Ballı Tuğsan , Yücesoy Mehmet , Bahçeci Halil İbrahim , Ülkü Abdulalh , Akçam Tolga , Polat Kamil Yalçın , Ekinci Nazım , Şimşek Halis , Örmeci Necati , Sonsuz Abdulalh , Demir Mehmet , Kılıç Murat , Uygun Ahmet , Demir Ali , Delik Anıl , Arslan Burcu , Doran Figen , Altıntaş Engin , Temel Tuncer , Bektaş Ahmet
Published: October 2018

The hepatocellular carcinoma (HCC) tumor marker alpha-fetoprotein (AFP) is only elevated in about half of the HCC patients, limiting its usefulness in following the effects of therapy or screening. New markers are needed. It has been previously noted that the inflammation markers C-reactive protein (CRP) and platelet-lymphocyte ratio (PLR) are prognostically important and may reflect HCC aggressiveness. We therefore examined these 2 markers in a low-AFP HCC cohort and found that for HCCs > 2 cm, both markers significantly rise with an increasing maximum tumor diameter (MTD). We calculated the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and Youden index value for each marker, and their area-under-the-curve values for each MTD group. Patients were dichotomized into 2 groups based on the CRP and PLR from the receiver-operating characteristic curve analysis. In the logistic regression models of the 4 different MTD patient groups, CRP and PLR levels were statistically significant to estimate MTD in univariate logistic regression models of MTD groups > 2 cm. CRP and PLR were then combined, and the combination was statistically significant to estimate MTD groups of 3-, 4-, and 5-cm cutoffs. CRP and PLR thus have potential as tumor markers for low-AFP HCC patients, and possibly for screening.