bluuebunny/crossref_metadata_2025 huggingface dataset:
Mapping the Evolution of Hotel Service Quality Research: A Bibliometric Perspective (1987–2024)
Authors: S Senathirajah Abdul Rahman Bin , Haque Rasheedul , Qazi Sayeeduz Zafar , Mehta Ahmed Muneeb , Jia Ye , Lertatthakornkit Tippawan
Published: December 2024

The purpose of this study is to analyze the publication trends and scholarly contributions related to hotel ser-vice quality from 1987 to 2024. Given the critical role service quality plays in shaping customer satisfaction, loyalty, and operational success in the hotel industry, understanding its research trajectory is essential for both academics and practitioners. This study utilizes a bibliometric analysis approach, extracting data from the Scopus database on publication metrics such as authors, countries, affiliations, sources, keywords, and refer-ences. Further visual mapping of keyword co-occurrence, author collaboration, and citation networks was con-ducted using Vosviewer to identify major trends and influential contributions in the field. Research on hotel service quality has expanded significantly over the past decades, with a steady increase in publications. Nota-bly, technological advancements, customer satisfaction models, and the integration of sustainability in service quality have emerged as key research themes. The period from 2020 onwards shows a particular focus on the effects of artificial intelligence and the impact of global crises, such as COVID-19, on hotel service quality. This study is limited to publications from 1987-2024, and while comprehensive, some seminal works may precede this time frame. Additionally, the study only includes articles indexed in Scopus, potentially excluding other important contributions. This study offers a holistic overview of the evolution of hotel service quality research, mapping its trajectory and highlighting emerging trends. It provides valuable insights into the role of service quality in driving operational excellence, guest satisfaction, and sustainable growth in the hospitality industry.
Purification of an FC-Fusion Protein with [Bathophenathroline:metal] Complexes
Authors: Patchornik Guy , Jayawickrama Thisara Withanage , Alcalay Ron , Krichevsky Olga , Wachtel Ellen , Mazor Ohad
Published: December 2024

In this study, we aim at assessing an alternative method for purification of Fc-fusion proteins that does not rely on chromatographic media or ligands. Recombinant human acetylcholinesterase, fused to the Fc-domain of human IgG1 (henceforth, AChE-Fc), was purified with precipitated aromatic complexes composed of the bathophenanthroline (henceforth, batho) chelator with either Zn2+ or Cu2+ ions (i.e., [(batho)3:Zn2+] or [(batho)3:Cu2+]) in the presence of polyethylene glycol 6000 (PEG-6000). In a three-step purification process conducted at pH 7, AChE-Fc was captured by the aromatic complexes (Step 1); unbound or weakly bound protein impurities were removed with 20 mM NaCl (Step 2); and AChE-Fc was then extracted at pH 7 (Step 3) using 100 mM Na citrate buffer in 250 mM NaCl. Purified AChE-Fc was monomeric (as determined by dynamic light scattering (DLS) and Native-PAGE); and it's secondary structure was maintained (by far UV circular dichroism (CD) spectroscopy). However, full enzymatic activity was only preserved with the [(batho)3:Zn2+] complex. Interaction between AChE-Fc and [(batho)3:Zn2+] led to ~83-88% overall protein yield. Process upscaling by 30-fold required only proportional increase in the amounts of [(batho)3:Zn2+] and PEG-6000. Efficient (95-97%) chelator recycling was achieved by recrystallization. Chelator leaching into purified AchE-Fc was estimated to be ~0.3% relative to the total amount used. Taken together, this novel procedure has the potential to provide an economical and practical avenue for industrial purification of Fc-fusion proteins.
Comparison of Commercially Available Thermostable DNA Polymerases with Reverse-Transcriptase Activity in Coupled Reverse-Transcription Polymerase Chain Reaction Assays
Authors: Smirnova Evgeniya V. , Blagodatskikh Konstantin A. , Barsova Ekaterina V. , Varlamov Dmitriy A. , Kramarov Vladimir M. , Ignatov Konstantin B.
Published: December 2024

Reverse-transcription polymerase chain reaction (RT-PCR) is an important tool for the detection of target RNA molecules and the assay of RNA pathogens. Coupled RT-PCR is performed with an enzyme mixture containing a reverse transcriptase and a thermostable DNA polymerase. To date, several biotechnological companies offer artificial thermostable DNA polymerases with a built-in reverse-transcriptase activity for use in the coupled RT-PCR instead of the enzyme mix-tures. Here, we compared the artificial DNA polymerases and conventional enzyme mixtures for the RT-PCR by performing end-point and real-time RT-PCR assays using severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV2) RNA and endogenous mRNA molecules as tem-plates. We found that the artificial enzymes were suitable for different RT-PCR applications, in-cluding SARS-CoV2 RNA detection, but not for long-fragment RT-PCR amplification.
Potential Applications of Computerised Algorithms and Implementation of AI Models into Microbiological, Evolutionary and Pharmaceutical Research
Authors: Carp Theodor-Nicolae
Published: December 2024

Artificial intelligence has sharply and broadly evolved since the invention of the computer and its eventual widespread distribution throughout the world. It has led to the replacement of traditional, long-term clinical practices to computerised procedures bringing rapid results. Ultimately, this led to the development of bioinformatics, helping geneticists sequence whole genomes and proteomes within a short time frame. Subsequently, biostatisticians receive novel tools to rapidly build statistical projections in a record amount of time as well. The overall key word behind computerised advancement of biological, genetic and medical research is “time”, as bioinformatics and biotechnology represent two key “enzymes” for the “chemical reaction” of clinical research. As a result, the field of epidemiology has received a novel, all-time opportunity to rapidly build statistical projections regarding the probability of future epidemic or even pandemic outbreaks, and the scope of the current paper is to academically raise awareness of such an opportunity, and to briefly describe examples through which such process could be performed from a general, biomedical science-based perspective. The central scope of the such research efforts could be the investigation of past single-nucleotide polymorphism events that have occurred in certain polymorphic viral agents responsible for the onset of clinically powerful infectious diseases, both indirectly, through the suppression of first-line and second-line host immunity, as well as directly, through the causation of clinical disease and even decrease in the quality of the induced third-line immune activity, to bring an overall system of scientific projection that will bring above-threshold levels of accuracy in the calculation of statistical probabilities regarding the onset of future epidemics and pandemics. It is important to observe the manner microbial agents substantially contribute to the onset of significant and severe clinical infectious diseases, not only by directly inducing the disease and targeting the central, adaptive elements of the host immune system, but also by causing a transient delay in the activation of the peripheral, innate elements of the host immunity, which play a critical role in the creation of an optimal bridge of signalling to the adaptive immunity, meaning that an optimal innate immune activation process plays a crucial role in a timely and qualitative activation of the adaptive immune system.
Radiofrequency Ablation for Locoregional Structural Incomplete Response in Differentiated Thyroid Cancer; initial experience in Greece
Authors: Simeakis George , Kapama Aikaterini , Paparodis Rodis D , Gkousis Pyrros , Koursaros Panayiotis , Kokkinis Christos , Zozolou Maria , Gkeli Myrsini
Published: December 2024

Background/Objectives: Structural incomplete response (SIR) (persistence/recurrence) may occur in 2-6% of low-risk differentiated thyroid cancer (DTC)-cases and in 67-75% of high-risk. Regarding locoregional disease, surgery is the optimal therapeutic modality if, the smallest dimension of the targeted node is ≥8mm or ≥10mm (central or lateral compartment). In the presence of smaller nodes, contraindications or, patient’s unwillingness for reoperation, active surveillance (AS) or minimally invasive treatments (MIT), may be considered. Methods: We retrospectively studied eight DTC patients with SIR confirmed by ultrasound (U/S)-guided fine-needle aspiration cytology (FNAC) and measurement of Thyroglobulin (Tg) in the washout fluid. 14 malignant lesions were ablated by radiofrequency (RF). We assessed prior to RF ablation (RFA) and consecutively at one month, three months and then every three months the volume of each lesion, serum Tg and AntiTg antibodies and calculated the volume reduction ratio (VRR). Results: Patients were followed for a mean period of 13.25 months (range: 4-24) after RFA was performed. The targeted lesions reduced significantly from a median volume of 0.24 mL (range: 0.09-0.9) to 0.02 mL (range: 0-0.03) (p<0.05), with a median VRR of 94.5% (range: 78-100%) and concomitant significant biochemical remission (decrease of serum Tg from a median of 1.05 ng/mL to 0.2 ng/mL, p<0.05). In one patient with an aggressive RAI-refractory histological variant re-recurrence was documented which was successfully re-ablated by RF. In two patients Horner syndrome was diagnosed as an RFA complication which was totally resolved within six months. Conclusions: RFA may be considered as an effective and safe MIT in selective DTC patients with SIR, especially in cases of smaller lesions. Additional prospective studies are needed including aggressive DTC histological variants towards a tailored therapeutic approach.
Information and Communication Technologies (ICTs): Opportunity in the Feminisation of Ageing
Authors: García Belén Reynaldos , Palacio María-Itatí , Lucas-Ochoa Ana-María , González-Cuello Ana-María , Fernández-Villalba Emiiano , Herrero María-Trinidad
Published: December 2024

Introduction: Population aging poses an unprecedented global challenge, giving rise to significant social, economic, and health-related complexities. The continuous growth in the older adult pop-ulation, coupled with the feminization of aging and the gender gap in healthcare access, under-scores the imperative to reformulate existing strategies for addressing aging. Current approaches often fall short in adequately addressing the unique characteristics of female aging. Methodology: A structured literature review was conducted as a data collection strategy. The search was conducted in May 2024, utilizing the Scopus, Web of Science, and PubMed databases. Ultimately, 157 articles were included in the review. Results: Information and Communication Technologies (ICTs) have emerged as a promising avenue for enhancing the quality of life of older adults. By providing as-sistance with daily tasks, stimulating cognitive function, facilitating social interaction, and miti-gating the detrimental effects of isolation and loneliness, ICTs empower older adults to maintain their independence. However, a persisting gender gap, which ICTs have not fully addressed, re-mains a significant challenge. Conclusions: To ensure the efficacy of strategies aimed at promoting healthy aging, it is imperative to democratize access to ICTs and bridge the existing gender gap. Consequently, novel technological solutions must be developed, adopting a person-centered ap-proach. This will facilitate the creation of a society where all older adults can enjoy fulfilling, healthy, and active lives, while simultaneously eliminating the inequalities faced by older women.
Primary objective: To assess the importance, need, and implementation of ICTs for improving older adults' health.
Secondary objectives: 1. To evaluate the applicability of a gender perspective. 2. To assess the potential of ICTs to improve quality of life and general well-being.
Assessing Urbanisation Trends, Urban Renewal and Sustainable Urban Development in Nigeria
Authors: EKEH ESEOGHENE , JEGEDE FOLUKE
Published: December 2024

he physical demonstration of the rapid growth in urbanisation in developing countries like Nigeria is often evidence of the social and economic transformations occurring in these countries. This paper aims to assess urbanisation trends, urban renewal, and sustainable urban development in Nigeria in view of the governmental policies that affect urbanisation trends, urban renewal, and sustainable urban development. The paper reviews current literature on the causes of global Urbanisation trends, the effects of urbanisation trends, and Urbanisation in Nigeria, which have resulted from rapid population growth and changes in the urban structure. By aligning urbanisation, urban renewal strategies, and policies that foster sustainable urban development with the Sustainable Development Goals (SDGs), Nigeria can work towards creating cities that promote the safety, inclusivity, resilience, and sustainability of its inhabitants.
A Comparison of Three Methodologies for Determining Soil Infiltrability in Thicketized Oak Woodlands and Adjacent Grasslands
Authors: Atalar Furkan , Leite Pedro , Wilcox Bradford Paul
Published: December 2024

This study had two primary objectives (1) to determine relative differences in soil infiltrability in thicketized oak woodlands; and (2) to compare the effectiveness of three infiltration measurement techniques—rainfall simulation, the Automated Simplified Steady Beerkan Infiltration (SSBI) method, and measurement by Saturo dual-head infiltrometer. Consistent with past studies in woody-plant-encroached rangelands, we found significantly higher infiltrability in the thicketized woodlands, with the greatest differences in areas characterized by clay soils and moderate grazing. With respect to measurement techniques, we found significant differences related to their unique strengths and limitations. Infiltrability results from rainfall simulation exhibited less variability than either the SSBI or the Saturo method, with coefficients of variability ranging from 15.5% to 54.3%. In addition, rainfall simulation was better able to detect differences in infiltrability between adjacent woodland and grassland sites. Infiltrability values and variability were both significantly higher with the Saturo method than with either of the other two, which did not differ significantly from one another (p = 0.627). Although rainfall simulation is probably the gold standard for determining soil infiltrability, it is labor-intensive and resource-demanding. The Saturo infiltrometer has the advantage of automated measurements, but it is bulky and prone to malfunctions. In contrast, the Automated SSBI method strikes a practical balance between efficiency and reliability, enabling multiple replicates with minimal water usage. Our findings have twofold importance: first, they demonstrate that thicketization of oak savannas results in higher soil infiltrability; and second, they demonstrate that the SSBI methodology is a suitable substitute for rainfall simulation for determining soil infiltrability.
The Theoretical Basis of qPCR and ddPCR Copy Number Estimates: A Critical Review and Exposition
Authors: McNair James N. , Frobish Daniel , Rediske Richard , Hart John , Jamison Megan , Szlag David
Published: December 2024

The polymerase chain reaction (PCR) is a molecular biology tool with diverse applications in the aquatic sciences. Classical PCR is a nonquantitative method that can be used to detect target DNA sequences that are characteristic of particular microbial taxa but cannot determine their concentrations in water samples. Various quantitative forms of PCR have been developed to remove this limitation. Of these, the two that currently are used most widely are real-time quantitative PCR (qPCR) and droplet digital PCR (ddPCR). Several outlines of the mathematical and statistical basis of these methods for estimating target sequence concentrations are available in the literature, but we are aware of no thorough and rigorous derivation of the theoretical underpinnings of either. The purpose of this review is to provide such derivations, and to identify and compare the main strengths and weaknesses of the two methods. We find that both estimation methods are sound, provided careful attention is paid to specific details that differ between the two. With qPCR, it is especially important to reduce any significant PCR inhibition by sample constituents and to properly fit the standard curve to heteroskedastic calibration data. With ddPCR, it is important to ensure that the value of the mean droplet volume used in calculating concentrations is correct for the particular combination of droplet generator and master mix used. Advantages of qPCR include lower instrument and per-sample costs, a shorter turnaround time for obtaining results, a higher upper limit of quantification, and a wider dynamic range. Advantages of ddPCR include freedom from dependence on a standard curve, inherently lower sensitivity to PCR inhibitors, a lower limit of quantification, a simpler theoretical basis, and simpler data analysis.
High-Frequency and Ultra-High-Frequency Ultrasound in Dermatologic Diseases and Aesthetic Medicine
Authors: Argalia Giulio , Reginelli Alfonso , Molinelli Elisa , Russo Anna , Michelucci Alessandra , Sechi Andrea , Marzano Angelo Valerio , Desyatnikova Stella , Fogante Marco , Patanè Vittorio , Granieri Giammarco , Tagliati Corrado , Rizzetto Giulio , De Simoni Edoardo , Matteucci Marco , Candelora Matteo , Lanza Cecilia , Ventura Claudio , Carboni Nicola , Esposito Roberto , Esposito Stefano , Paolinelli Massimiliano , Esposto Elisabetta , Lanni Giuseppe , Lucidi Pressanti Gabriella , Giorgi Chiara , Principi Fabiola , Rebonato Alberto , Malinowska Sylwia Patrycja , Mlosek Robert Krzysztof , Giuseppetti Gian Marco , Dini Valentina , Romanelli Marco , Offidani Annamaria , Cappabianca Salvatore , Wortsman Ximena , Simonetti Oriana
Published: December 2024

Dermatologic ultrasonography applications are rapidly growing in all skin fields. Thanks to very high spatial resolution, high-frequency and ultra-high-frequency ultrasound can evaluate smaller structures allowing us to improve diagnosis accuracy and disease activity. Moreover, they can guide treatment, such as drug injection, and assess therapy efficacy and complications. In this narrative review, we evaluated high-frequency ultrasound and ultra-high-frequency ultrasound in infections, inflammatory dermatoses, metabolic and genetic disorders, specific cutaneous structure skin disorders, vascular and external agents associated disorders, neoplastic diseases, and aesthetics.
Dragon Intermittency at the Transition to Synchronization in Coupled Rulkov Neurons
Authors: Bashkirtseva Irina A. , Ryashko Lev B. , Pisarchik Alexander N.
Published: December 2024

We investigate synchronization dynamics of two non-identical, mutually coupled Rulkov neurons, emphasizing the effects of coupling strength and parameter mismatch on the system’s behavior. At low coupling strengths, the system exhibits multistability, characterized by the coexistence of three distinct 3-cycles. As the coupling strength is increased, the system becomes monostable with a single 3-cycle remaining as the sole attractor. A further increase in the coupling strength leads to chaos, which we identify as arising through a novel type of intermittency. This intermittency is characterized by alternating dynamics between two low-dimensional invariant subspaces: one corresponding to synchronization and the other to asynchronous behavior. We show that the system’s phase-space trajectory spends variable durations near one subspace before being repelled into the other, revealing non-trivial statistical properties near the onset of intermittency. Specifically, we find two key power-law scalings: (i) the mean duration of the synchronization interval scales with the coupling parameter, exhibiting a critical exponent of −0.5 near the onset of intermittency, and (ii) the probability distribution of synchronization interval durations follows a power law with an exponent of −1.7 for short synchronization intervals. Intriguingly, for each fixed coupling strength and parameter mismatch, there exists a most probable super-long synchronization interval, which decreases as either parameter is increased. We term this phenomenon "dragon intermittency" due to the distinctive dragon-like shape of the probability distribution of synchronization interval durations.
Perspectives and Tools in Liver Graft Assessment: A Transformative Era in Liver Transplantation
Authors: Safi Kawthar , Pawlicka Angelika Joanna , Pradhan Bhaskar , Sobieraj Jan , Zhylko Andriy , Struga Marta , Grat Michal , Chrzanowska Alicja
Published: December 2024

Liver transplantation is a critical and evolving field in modern medicine, offering life-saving treatment for patients with end-stage liver disease and other hepatic conditions. Despite its transformative potential, transplantation faces persistent challenges, including a global organ shortage, increasing liver disease prevalence, and significant waitlist mortality rates. Current donor evaluation practices often discard potentially viable livers, underscoring the need for refined graft assessment tools. This review explores advancements in graft evaluation and utilization aimed at expanding the donor pool and optimizing outcomes. Emerging technologies, such as imaging techniques, dynamic functional tests, and biomarkers, are increasingly critical for donor assessment, especially for marginal grafts. Machine learning and artificial intelligence, exemplified by tools like LiverColor, promise to revolutionize donor-recipient matching and liver viability predictions, while bioengineered liver grafts offer a future solution to the organ shortage. Advances in perfusion techniques are improving graft preservation and function, particularly for donation after circulatory death (DCD) grafts. While challenges remain—such as graft rejection, ischemia-reperfusion injury, and recurrence of liver disease—technological and procedural advancements are driving significant improvements in graft allocation, preservation, and post-transplant outcomes. This review highlights the transformative potential of integrating modern technologies and multidisciplinary approaches to expand the donor pool and improve equity and survival rates in liver transplantation.
Perch Hydrolysates from Upcycling of Perch Side Streams Accelerate Wound Healing Through Enhancing Fibroblasts to Secrete Procollagen I, Fibronectin and Hyaluronan
Authors: Chang Jia-Feng , Hsieh Chih-Yu , Chen Ling-Ni , Lee Mao-Hsiang , Ting Yi-Han , Yang Chi-Yu , Lin Chih-Cheng
Published: December 2024

Wound healing incurs various challenges, making it a paramount topic in medicine. Short chain peptides from fish protein hydrolysates pose wound healing properties that may bring a solution. In this study, perch hydrolysates were produced from perch side steams using a designed commercial complex enzyme via a proprietary pressure extraction technique. The average molecular weight of the perch peptides was 1,289 kDa, and 62.60% of the peptides had a low molecular weight (≤ 1 kDa). The beneficial amino acid sequence FPSLVRGP with antihypertensive effects accounted for 6.21% abundance. The concentrations of collagen composition and branched chain amino acids was 1183 and 1122 mg/100g, respectively. In fibroblast model, active perch peptides accelerated wound healing mainly by increasing the secretion of procollagen I, fibronectin, and hyaluronan. In SD rat model to mimic human wound, orally perch hydrolysates with a molecular weight below 2.3 kDa accelerating wound healing mainly resulted from collagen-forming amino acids, branched-chain amino acids, and matrikine-like peptides. Collectively, the residue of the perch extract can be upcycled via hydrolysis technique to produce not only bioactive sequences but also short chain peptides. Considering the therapeutic potential to promote wound healing, such by-products are of great value that may develop as dietary nutraceuticals.
Analysis of Differences in the Classification of Endometrial Cancer Patients in Poland
Authors: Szatkowski Wiktor , Kluz Tomasz , Cieślak-Steć Małgorzata , Śliwińska Magdalena , Nowak-Jastrząb Małgorzata , Winkler Izabela , Tomaszewski Jacek , Blecharz Paweł
Published: December 2024

Background: Endometrial cancer (EC) incidence and mortality have been steadily rising globally over recent decades. The introduction of advanced molecular technologies, such as next-generation sequencing (NGS), alongside the FIGO 2023 classification, presents opportunities for refined diagnostics and risk stratification. This study aimed to analyze differences in EC classification among oncology centers in southeastern Poland.
Methods: Data were collected from 461 consecutive patients newly diagnosed with EC between 2022 and 2024 at four major oncology centers in southeastern Poland. Molecular and immunohistochemical (IHC) analyses were conducted on formalin-fixed paraffin-embedded (FFPE) tissues to identify key markers, including POLE mutations, MSI-H, and p53 status.
Results: The application of the FIGO 2023 staging system revealed statistically significant inter-center differences, with Centers 1 and 4 diagnosing a higher proportion of early-stage cases. The most prevalent subtype was NSMP, observed in 51% of cases. MSI-H occurred in 13%-36% of patients, depending on the center. p53 mutations ranged from 9% to 26%. POLE mutations were identified in 4% overall. Significant variations in molecular subtype distribution across centers highlight potential differences in diagnostic access or tumor biology. Conclusions: The findings demonstrate regional differences in EC staging and molecular profiles in Poland, potentially reflecting disparities in diagnostic resources, methodologies, or tumor characteristics. Addressing these variations through standardized diagnostic protocols and equitable access to molecular tools is critical for optimizing patient outcomes. Future research should focus on evaluating the impact of molecular markers on therapy response and prognosis to guide personalized treatment strategies.
Resources and Readmission for COPD Exacerbation in Pneumology Units in Spain: The COPD Observatory Project
Authors: Calle Rubio Myriam , Cebollero Rivas Pilar , Esteban Cristóbal , Fuster Gomila Antonia , García Guerra José Alfonso , Golpe Rafael , Hernández Hernández Jesús R. , Lozada Bonilla Jessica Sara , Figueira-Gonçalves Juan Marco , Márquez Eduardo , Martínez Garceran José Javier , de Miguel-Díez Javier , Pando-Sandoval Ana , Riesco Juan A. , Santos Pérez Salud , Sánchez-del Hoyo Rafael , Rodríguez Hermosa Juan Luis
Published: December 2024

Objective: To describe resources, performance and care provided in pulmonology units in Spain, assessing their association with 30-day readmission for Chronic obstructive pul-monary disease (COPD) and in-hospital mortality. Methods: This survey was conducted on 116 responsible for the COPD pathway in Pneumology Units/Services from November 2022 to March 2023. Results: Of the 116 participating hospitals, 56% had a pulmonology service and 25.9% had pneumology section. The great majority were public and university hospitals. The number of beds allocated to pulmonology/100,000 inhabitants was 6.6 (3.1-9.2) and pulmonologist staffing was 3.3 (2.6-4.1) per-100,000 inhabitants. In 31.9% of pulmonology unit, there was Intermediate Care Unit dependiente (IMCU) and respiratory team for 24-hour emergency care in 30%, while only 9.5% had interventional pulmonology units. COPD rehabilitation programmes were offered in 58.6% of pulmonology units. The average rate of patients on ventilatory support in acute failure was 13.8 (9.2-25) per-100 discharges, with a 30-day COPD readmission rate of 14.9% with significant differences according to the level of complexity (p=0.041), with a mean length of stay of 8.72 (1.26) days. The overall in-hospital mortality in pulmonology units was 4.10 (1.18) per-100 admissions. In the adjusted model having a discharge support programme and interventions performed during admission (number of patients with ventilatory support) were predictors of a favourable outcome. Hospital stay was also maintained as a predictor of an unfavourable outcome. Conclusions: Knowledge of resources and outcomes in the COPD process will allow appropriate planning to improve the quality of care.
Multiple Highly Methylated CpG Sites as Potential Epigenetic Markers for the Diagnosis of Prostate Cancer
Authors: Jean-Pierre Roperch , Charbonnier Guillaume , Figiel Sandy , Lamb Alastair , Mills Ian , Hennion Claude , Cancel-Tassin Géraldine , Cussenot Olivier
Published: December 2024

Prostate cancer represents the second most frequently diagnosed malignant tumour in males. Serum PSA is used as a screening tool for prostate cancer. However, there are limitations to this approach due to the number of false positives it generates, which can result in overdiagnosis and over-treatment. The objective of this study was to identify accurate DNA methylation markers for the diagnosis of prostate cancer. In order to achieve this objective, whole-methylome sequencing was performed on tumour tissue samples, and the identified epi-genetic markers were validated using multiplex methylation-specific PCR. Abstract: Background/Objectives: Prostate cancer (PCa) remains the leading cause of cancer deaths in men. Serum Prostate-Specific Antigen (PSA) test is widely used for PCa screening. This method is controversial, as it can lead to over-diagnosis and over-treatment. Using DNA methyl-ation sequencing, our aim was to identify new sensitive and specific epigenetic markers in PCa tissue. Methods: DNA methylome analysis was performed from 15 paired tumors (T) and non-tumour adjacent (NT) prostate tissues by Enzymatic Methyl-seq Kit (EM-seq). Results: 66 CpGs sites representing eight genes were identified as hypermethylated in T versus NT, and were confirmed by multiplex methylation-specific PCR (MM-SPCR). A very good correlation between EM-Seq and MM-SPCR results was observed (Pearson's correlation of 0.93). As an indicator of the overall methylation status, the percentage of methylated reference (PMR) was measured for each marker. Overall, a significant difference was found in the mean PMR values of T vs. NT (86.2  30.3% vs. 5.0  3.2%, p < 0.0001). Area under the ROC curve (AUC) was calculated from PMRs for each marker, with AUC values ranging from 0.987 to 1.0. This is an indication of the very high diagnostic accuracy of these markers in the tissue of prostate cancer patients. Conclusions: A total of 66 hypermethylated CpG sites were identified in paired prostate tumour tissue in comparison to non-tumour adjacent tissue by EM-seq. They represent a set of eight genes, including CLDN5, GSTP1, NBEAL2, PRICKLE2, SALL3, TAMALIN, TJP2 and TMEM106A, which could be used as diagnostic markers for prostate cancer.
Integrating Metadiscourse Analysis with Transformer-Based Models for Enhancing Construct Representation and Discourse Competence Assessment in L2 Writing: A Systemic Multidisciplinary Approach
Authors: Chan Sathena , Sathyamurthy Manoranjan , Inoue Chihiro , Bax Michael , Jones Johnathan , Oyekan John
Published: December 2024

In recent years, large-scale language test providers have developed or adapted automated essay scoring systems (AESS) to score L2 writing essays. While the benefits of using AESS are clear, they are not without limitations, such as over-reliance on frequency counts of vocabulary and grammar variables. Discourse competence is one important aspect of L2 writing yet to be fully explored in AEE application. Evidence of discourse competence can be seen in the use of Metadiscourse Markers (MDM) to produce reader-friendly texts. The article presents a multidisciplinary study to explore the feasibility of expanding the construct representation of automated scoring models to assess discourse competence in L2 writing. Combining machine learning, automated textual analysis and corpus-linguistic methods to examine 2000 scripts across two tasks and five proficiency levels, the study investigates (1) in addition to frequency and range, whether accuracy of MDM is worth pursuing as a predictive feature in L2 writing, and (2) how identification and classification of MDM use might be fed into developing an automated scoring model using machine learning techniques. The contributions of this study are three-fold. Firstly, it offers valuable insights within the context of Explainable AI. By integrating MDM usage and accuracy into the scoring framework, this research moves beyond frequency-based evaluation. This study also makes significant contributions to the current understanding of L2 writing development that even lower-proficiency learners exhibit evidence of discourse competence through their accurate use of MDMs as well as their choice of MDMs in response to genre. From the perspective of expanding the construct representation in automated scoring systems, this study provides a critical examination of the limitations of many AEE models, which have heavily relied on vocabulary and grammar features. By exploring the feasibility of incorporating MDMs as predictive features, this research demonstrates the potential for construct expansion of L2 AEE. The results would support test providers in developing competence tests in various contexts and domains including manufacturing, medicine and so on.
Fragilidad y mortalidad en pacientes con enfermedad renal crónica en hemodiálisis en tres clínicas del MINSA
Authors:   , Catacora Juanillo Johanan Madelayne , Chavez Revoredo Tonio Gustavo
Published: December 2024

Objetivo: Evaluar si la fragilidad es un riesgo para mortalidad y hospitalización en pacientes con enfermedad renal crónica grado 5 en tres clínicas. Metodología: Estudio tipo analítico de cohorte prospectivo, que recopilará datos a través de fichas de datos y del cuestionario FRAIL para evaluar la fragilidad en pacientes con enfermedad renal crónica. grado 5 en hemodiálisis en tres clínicas tercerizadas de hemodiálisis del MINSA en el periodo de 6 meses, Se empleó un muestreo por conveniencia en tres clínicas, con un promedio de 240 pacientes. Se incluyó a 206 pacientes que aceptaron participar en el estudio, Tomando en cuenta un 15% extra. por posibles pérdidas durante el estudio. Los pacientes fueron divididos según la presencia de fragilidad. También se recopilaron variables clínicas, como edad, antecedentes (Diabetes Mellitus 2, hipertensión) y tiempo en diálisis, así como datos de exámenes de laboratorio como albumina y hemoglobina, obtenidos de las historias clínicas. Las variables prospectivas evaluadas incluyeron mortalidad intrahospitalaria, mortalidad extrahospitalaria y hospitalizaciones. Se empleó el software. STATA 17. Resultados: En relación con la mortalidad, se observa que el 97.1% de los pacientes no ha fallecido durante el periodo de estudio. En relación con la hipertensión arterial, todos los pacientes que fallecieron la presentaban en contraste con solo una pequeña fracción de los pacientes no fallecidos. Respecto a la diabetes mellitus tipo 2, la proporción es idéntica entre los fallecidos y los que no fallecieron. La variable de hospitalización revela una alta proporción de pacientes fallecidos había sido hospitalizada (83.33%), en comparación con un 18.09% de los pacientes no fallecidos. Esto sugiere que la hospitalización está vinculada con el fallecimiento. La proporción de pacientes con hipertensión arterial es alta con mayor prevalencia en los pacientes frágiles (90.45%) comparado con los no frágiles (88.89%)...
Voices of the Xingu: Community-based monitoring reveals the impacts of the permanent drought imposed to an Amazonian River by the Belo Monte Hydroelectric Power Plant
Authors: Assunção Amildon Moura , Silva Anderson Sampaio da , Silva Helio Bezerra da , Juruna Jailson Jacinto Pereira , Nunes Josimary Abreu , Kleme Micaele Souza Santos , Ferreira Paulo Passos , Juruna Ronald Txakui Viana da Silva , Santos Rosilene Sousa dos , Lima Sara Rodrigues , Lima Sebastião Bezerra , Pereira Tarukawa Juruna da Cruz , Quaresma Adriano , de Queiroz Alexya Cunha , Sawakuchi André Oliveira , Ribas Camila Cherem , Ritter Camila Duarte , Carneiro Cristiane Costa , De Paula Eder Mileno Silva , Zuquim Gabriela , Wahnfried Ingo , de Jesus Jandessa Silva , Muriel-Cunha Janice , Zuanon Jansen , Pezzuti Juarez Carlos Brito , de Medeiros Marksuel Sandro Silva , Lopes Priscila F. M. , Mantovanelli Thais Regina
Published: December 2024

The Belo Monte Hydroelectric Power Plant (HPP) relies on diverting water from the Xingu River (Brazilian Amazon) to generate energy, disrupting the natural flooding cycle and creating an extreme and extended dry period along a 130 km stretch of the Xingu River (Volta Grande do Xingu - VGX). Local communities, including ribeirinhos (riverside dwellers) and indigenous peoples anticipated irreversible impacts on their lives and the river's ecosystems well before the dam's construction. In their words, "since before the Belo Monte dam was built, we … knew that our lives, as well as our river, would suffer irreversible impacts. Prior to the dam, we experienced the harmony of the forest and the synchrony of the reproductive cycle of the shoals of countless fishes’ species.” Residents observed the transformation firsthand, noting that now there is “a water shortage, which leaves our river without the strength to provide us with life". The environmental licensing process for the HPP overlooked the vital link between the river's flow, the local ecosystems, and the traditional lifestyles of indigenous and ribeirinhoscommunities, prompting residents to establish the Independent Territorial Environmental Monitoring Program (MATI-VGX) in collaboration with academic researchers. The community's initiative sheds light on the drastic transformations, including shifts in fishing practices and a significant decline in fishing efficiency, jeopardizing local autonomy and food security. Here we present evidence of disruptions in ecological cycles affecting aquatic and seasonally flooded ecosystems, which are intrinsically connected to the modes of life of local populations. The research underscores the need to increase water discharge to the VGX, and the indispensable role of local communities in decision making regarding impact assessment, mitigation, and monitoring. Residents voiced this by stating, "We want to show that our knowledge should never be ignored in projects in which we are most affected.".
Comprehensive One Health Assessment: Knowledge, Attitudes, and Practices (KAPs) among Livestock and Poultry Farmers about Zoonosis in Bangladesh
Authors: Ahmed Md Jisan , Bhuiyan Md Ismile Hossain , Chalise Ritu , Mamun Mustakim , Bhandari Prajwal , Islam Khairul , Jami Shahrin Sultana , Ali Mahabbat , Sabrin Mirza Synthia
Published: December 2024

In Bangladesh, farming is a key livelihood, leading to a higher risk of zoonotic diseases due to frequent animal interactions and traditional practices. The study aimed to assess the knowledge, attitudes, and practices of livestock farmers regarding zoonotic disease outbreaks. A cross-sectional study of 658 livestock farmers from randomly selected regions in Bangladesh was conducted from April to June 2024. The participants included farmers with at least two livestock animals and at least ten birds on their farms. Among livestock farmers, 89.06% were male, mostly aged 30–45, but mainly from nonscience educational backgrounds. A survey showed significant knowledge and practice gaps regarding zoonosis, with only 11.55% having good knowledge and 37.54% demonstrating good practices. Farmers with a science education had higher KAP scores. Most avoided consuming raw animal products, though 23% lived with animals. Logistic regression identified factors influencing KAP, including rearing systems, farming experience, pet ownership, and biosecurity training. The study found that farmers lack adequate knowledge and practices regarding zoonosis, despite having a positive attitude. The study emphasizes the need for targeted educational programs and a collaborative One Health approach to improve zoonotic disease control, particularly among less educated farmers, to boost public health and economic outcomes.
Stent as A Bridge to Surgery for Malignant Colonic Obstruction: a retrospective study on survival and outcomes
Authors: Wong Thanawin , Pattarapuntakul Tanawat , Netinatsunton Nisa , Sottisuporn Jaksin , Yaowmaneerat Thanapon , Chaochankit Wongsakorn , Attasaranya Siriboon , Sripong Pimsiri , Chamroonkul Naichaya , Chongsuvivatwong Viraksakdi
Published: December 2024

Background In cases of malignant colonic obstruction (MCO), self-expandable metallic stents (SEMS) are used as a bridge to surgery, offering an alternative to emergency surgery. However, the long-term oncologic outcomes remain debated, particularly in developing countries where the cost of SEMS is a concern. This study aimed to evaluate overall survival (OS) and outcomes associated with SEMS as a bridge to surgery (SBTS) compared to direct emergency surgery (ES) in patients with acute MCO. Methods A retrospective study was conducted, including patients with potentially curable obstructed colon cancer who were treated with either STBS or ES at a university hospital in Thailand from 2015 to 2022. We compared OS, 5-year OS, disease-free survival (DFS), postoperative morbidity, and complications between the SBTS and the ES groups. Results A total of 106 patients were eligible, 29 underwent SBTS, and 77 underwent ES. Baseline characteristics were similar except for ASA classification and chemotherapy rates. The median OS was 56.1 months, with no significant differences in OS (51.4 vs. 61.0 months, p = 0.67) or 5-year DFS (53.8% vs. 59.9%, p = 0.32) between the two groups. The SBTS group had higher rates of minimally invasive surgery (MIS) (65.5% vs. 16.9%, p < 0.001) and shorter postoperative stays (POS) (7 vs. 9 days, p = 0.026). Stage IV cancer and low serum albumin were poor prognostic factors for OS. Conclusion SEMS placement as a bridge to surgery had no significant impact on OS compared to ES, but it was associated with shorter hospital stays and higher rates of MIS.
Central nervous system depressant outcomes of Artemisia monosperma Delile on male rat brain: Phytochemical analysis
Authors: Zeid Ehsan Abu
Published: December 2024

The current study aimed to examine the neurobehavioral activity of Artemisia monosperma Delile (ARM) ethanolic extract in adult male rats. Phytochemicals was screened using GC˗MS and HPLC analysis. ARM’s acute oral toxicity was determined. The study included four equal groups (n=12): the 1st Control, the 2nd Diazepam (DIZ), the 3rd ARM-high group (ARM-H), and the 4th ARM-low group (ARM-L). The ARM extract effect on the CNS activity was evaluated via behavioral tests, brain neurotransmitters and GABAA receptors (GABARAP). HPLC analysis revealed the presence of coffeic acid, rutin, gallic acid, and querectin. In the open field test (OFT), ARM significantly (p < 0.001) decreased immobility time and line crossings. ARM significantly (p < 0.05) increased the brain γ-aminobutyric acid (GABA). ARM significantly (p < 0.05) up-regulated the brain mRNA expression levels of GABARAP and BDNF, meanwhile, MAOA levels were significantly (p < 0.05) downregulated. For the first time, A. monosperma ethanolic extract might keep CNS depressant and antioxidative effects which could be attributable to its phytochemicals. Further toxicological and pharmacological studies are required for the semi-purified phytochemicals.
Analysis of spinal motion range, thoracic mobility, and abdominal muscle strength in patients operated on for renal cell carcinoma – a pilot, observational cross-sectional study at 3 to 5 years after the surgery
Authors: Głowacka-Mrotek Iwona , Jankowski Michał , Tarkowska Magdalena , Ratuszek-Sadowska Dorota , Leksowski Łukasz , Lewandowska Anna , Porzych Piotr , Jarzemski Piotr , Nowikiewicz Tomasz , Mackiewicz-Milewska Magdalena
Published: December 2024

Purpose
With the prolongation of overall survival in patients with renal cell carcinoma, evaluation of the impact of the type of surgical procedure on patients’performance status is gaining particular importance. The purpose of this study was to analyze the balance and spinal motion range in patients having undergone total or partial renal resection.
Methods
This cross-sectional study was carried out ina group of patients having received surgical treatment for renal cell carcinoma at the Department of Urology of the Dr. Jan Biziel University Hospital No. 2 in Bydgoszcz 3 to 5 years prior to the inclusion. A total of 31 patients had undergone nephron-sparing surgery [NSS] while another 33 patients had undergone radical nephrectomy [RN]. The control group [CG] consisted of 24 age-range-matched patients. The purpose of the study was to evaluate the range of motion in the spinal joints, thoracic mobility, and abdominal muscle strength.
Results
Statistical analysis revealed highly significant differences between the study groups as regards the following parameters: total spine flexion (p = 0.0001), thoracic spine flexion (p < 0.0001), lumbar spine flexion (p < 0.0001), thoracic mobility (inhalation vs. exhalation) (p < 0.0001), rectus and oblique abdominal muscle strength (p < 0.0001). In all the aforementioned parameters, higher scores corresponded to better mobility. Higher scores and better range of motion in spinal joints were observed in the control group. No statistically significant differences between the groups (NSS, RN and CG) were observed in lumbar spine extension (backward inclination) (p > 0.05).
Conclusions
Surgical treatment within the kidneys is associated with reduction in the range of spinal motion, thoracic mobility, and abdominal muscle strength regardless of the type of surgery performed (nephron-sparing surgery vs. radical nephrectomy).
High Intra-tumoral and Sera Matrix Metalloproteinase 9 Levels Reduce Glioblastoma and Brain Metastases Patients' Survival
Authors: Kaisman-Elbaz Tehila , Haddad-Shlaifshtein Snir , Eskira Yael , Merkin Vladimir , Dumanis Guy , Turiel Sivan , Atar-Vardi Maya , Bari Romi , Alt Adi , Zamed Tali , Rotem-Dai Noa , Lavrenkov Konstantin , Kezerle Yarden , Dyomin Victor , Razon Ronit , Chakraborty Moumita , Asraf Hila , Hershfinkel Michal , Melamed Israel
Published: December 2024

Purpose: Matrix metalloproteinase 9 (MMP-9) has been shown to induce glioblastoma invasion and brain metastases (BM) spread. However, its clinical significance for monitoring disease progression has yet to be established. This study evaluates intra-tumoral and sera MMP-9 levels and their correlation to glioblastoma and BM patients' overall survival (OS).
Methods: 69 tumor and pre-operative sera samples were obtained from the brain tumor bank of the neurosurgery department at Soroka University Medical Center from patients who underwent tumor resection between 2015 and 2021. Clinical and imaging data from 27 glioblastoma and 30 BM patients were analyzed, and their MMP-9 levels and activity were measured and compared with 12 meningioma patients and 23 healthy subjects. Survival analyses were performed to examine MMP-9 level, activity, and clinical parameters' correlation with patients' OS.
Results: Glioblastoma and BM patients demonstrated increased median intra-tumoral MMP-9 levels (8ng/ml and 4ng/ml, respectively, p<0.001), activity, and pre-operative sera levels (2.8ng/ml and 1.8ng/ml, respectively, p<0.001). MMP-9 was specifically detected within and between glioblastoma cells and tumor endothelia. High intra-tumoral and sera MMP-9 levels, but not its activity, were linked to decreased OS in glioblastoma and BM patients (15.8 versus 8.4 months, p=0.022). MMP-9 was readily measured in patient sera.
Conclusions: This study suggests that intra-tumoral and sera MMP-9 can assist in identifying glioblastoma and BM recurrence/progression and that high intra-tumoral and/or sera MMP-9 levels at diagnosis correlate with significantly shorter patient OS. Importantly, sera MMP-9 could be longitudinally and non-invasively monitored in those patients and, once rising, may indicate tumor progression.