bluuebunny/crossref_metadata_2025 huggingface dataset:
An instrument to measure atrial fibrillation knowledge in Chinese patients: validation of the Jessa Atrial fibrillation Knowledge Questionnaire
Authors: Chang Sijie , Xu Wenlin , Wu Shuyi , Desteghe Lien , Zhang Feilong , Zhang Jinhua
Published: June 2023

Background: There is no validated tool to assess patients’ knowledge of oral anticoagulant therapy in atrial fibrillation in China.

Methods: Using a standard translation program, the Jessa Atrial fibrillation Knowledge Questionnaire (JAKQ) was translated into Chinese. The reliability of the JAKQ was assessed by internal consistency (Cronbach’s α coefficient), repeatability (test-retest reliability), and sensitivity tests. Effectiveness was assessed by hypothesizing that a lower JAKQ score was a risk factor for bleeding. A total of 447 patients with atrial fibrillation (AF) who were hospitalized between July 2019 and December 2021 were studied and followed up. Participants were followed up 1, 3, 6, and 12 months after enrollment. Bleeding during follow-up was recorded. Data were obtained from hospital databases and telephone follow-up.

Result: A total of 447 patients with AF completed JAKQ. The mean age of patients was 67.7 ± 10.2 years. The median JAKQ score was 31.3% (12.5–43.8). The Cronbach’s α coefficient of JAKQ was 0.616–0.637, and the test-retest reliability value was 0.902 (p < 0.001). Multivariate logistic regression showed that the higher knowledge level of AF was associated with secondary education or above, an income of more than 2000 yuan, and a history of AF of more than 1 year. Bleeding was associated with a lower JAKQ score, hypertension, and a history of bleeding. Non-bleeding patients on VKA had a better understanding of how often INR should be monitored and what to do if an OAC dose was missed.

Conclusion: The Chinese version of JAKQ shows good reliability and validity, indicating that it is a valuable tool for AF and oral anticoagulation (OAC) knowledge assessment. It can be used in clinical practice to guide educational activities and improve the effectiveness and safety of treatment. It was shown that Chinese patients with AF have insufficient knowledge about AF and OAC. Lower JAKQ scores are associated with bleeding, so targeted education is necessary. Targeted educational efforts should focus on patients recently diagnosed with AF and those with lower formal education and income.
A tricarboxylic acid cycle-based machine learning model to select effective drug targets for the treatment of esophageal squamous cell carcinoma
Authors: Liang Yicheng , Tan Binghua , Du Minjun , Wang Bing , Gao Yushun , Wang Minghui
Published: June 2023

Background: The tricarboxylic acid cycle (TCA cycle) is an important metabolic pathway and closely related to tumor development. However, its role in the development of esophageal squamous cell carcinoma (ESCC) has not been fully investigated.

Methods: The RNA expression profiles of ESCC samples were retrieved from the TCGA database, and the GSE53624 dataset was additionally downloaded from the GEO database as the validation cohort. Furthermore, the single cell sequencing dataset GSE160269 was downloaded. TCA cycle-related genes were obtained from the MSigDB database. A risk score model for ESCC based on the key genes of the TCA cycle was built, and its predictive performance was evaluated. The association of the model with immune infiltration and chemoresistance were analyzed using the TIMER database, the R package “oncoPredict” score, TIDE score and so on. Finally, the role of the key gene CTTN was validated through gene knockdown and functional assays.

Results: A total of 38 clusters of 8 cell types were identified using the single-cell sequencing data. The cells were divided into two groups according to the TCA cycle score, and 617 genes were identified that were most likely to influence the TCA cycle. By intersecting 976 key genes of the TCA cycle with the results of WGCNA, 57 genes significantly associated with the TCA cycle were further identified, of which 8 were screened through Cox regression and Lasso regression to construct the risk score model. The risk score was a good predictor of prognosis across subgroups of age, N, M classification and TNM stage. Furthermore, BI-2536, camptothecin and NU7441 were identified as possible drug candidates in the high-risk group. The high-risk score was associated with decreased immune infiltration in ESCC, and the low-risk group had better immunogenicity. In addition, we also evaluated the relationship between risk scores and immunotherapy response rates. Functional assays showed that CTTN may affect the proliferation and invasion of ESCC cells through the EMT pathway.

Conclusion: We constructed a predictive model for ESCC based on TCA cycle-associated genes, which achieved good prognostic stratification. The model are likely associated with the regulation of tumor immunity in ESCC.
The macrocyclic lactone oxacyclododecindione reduces fibrosis progression
Authors: Saurin Sabrina , Meineck Myriam , Rohr Markus , Roth Wilfried , Opatz Till , Erkel Gerhard , Pautz Andrea , Weinmann-Menke Julia
Published: June 2023

Background: Renal fibrosis is one of the most important triggers of chronic kidney disease (CKD), and only a very limited number of therapeutic options are available to stop fibrosis progression. As fibrosis is characterized by inflammation, myofibroblast activation, and extracellular matrix (ECM) deposition, a drug that can address all these processes might be an interesting therapeutic option.

Methods: We tested in vivo in an ischemia–reperfusion (I/R) model in C57BL/6 mice and in kidney tubular epithelial cells (TEC) (HK2 cell line and primary cells) whether the natural product oxacyclododecindione (Oxa) reduces fibrosis progression in kidney disease. This was evaluated by Western blot, mRNA expression, and mass spectrometry secretome analyses, as well as by immunohistochemistry.

Results: Indeed, Oxa blocked the expression of epithelial–mesenchymal transition marker proteins and reduced renal damage, immune cell infiltration, and collagen expression and deposition, both in vivo and in vitro. Remarkably, the beneficial effects of Oxa were also detected when the natural product was administered at a time point of established fibrotic changes, a situation close to the clinical situation. Initial in vitro experiments demonstrated that a synthetic Oxa derivative possesses similar features.

Conclusion: Although open questions such as possible side effects need to be investigated, our results indicate that the combination of anti-inflammatory and anti-fibrotic effects of Oxa make the substance a promising candidate for a new therapeutic approach in fibrosis treatment, and thus in the prevention of kidney disease progression.
Transcriptome analysis provides insights into the cell wall and aluminum toxicity related to rusty root syndrome of Panax ginseng
Authors: Tong Aizi , Liu Wei , Wang Haijiao , Liu Xiaoliang , Xia Guangqing , Zhu Junyi
Published: June 2023

Rusty root syndrome is a common and serious disease in the process of Panax ginseng cultivation. This disease greatly decreases the production and quality of P. ginseng and causes a severe threat to the healthy development of the ginseng industry. However, its pathogenic mechanism remains unclear. In this study, Illumina high-throughput sequencing (RNA-seq) technology was used for comparative transcriptome analysis of healthy and rusty root-affected ginseng. The roots of rusty ginseng showed 672 upregulated genes and 526 downregulated genes compared with the healthy ginseng roots. There were significant differences in the expression of genes involved in the biosynthesis of secondary metabolites, plant hormone signal transduction, and plant–pathogen interaction. Further analysis showed that the cell wall synthesis and modification of ginseng has a strong response to rusty root syndrome. Furthermore, the rusty ginseng increased aluminum tolerance by inhibiting Al entering cells through external chelating Al and cell wall-binding Al. The present study establishes a molecular model of the ginseng response to rusty roots. Our findings provide new insights into the occurrence of rusty root syndrome, which will reveal the underlying molecular mechanisms of ginseng response to this disease.
Cross-sectional field study comparing hippocampal subfields in patients with post-traumatic stress disorder, major depressive disorder, post-traumatic stress disorder with comorbid major depressive disorder, and adjustment disorder using routine clinical data
Authors: Knaust Thiemo , Siebler Matthias B. D. , Tarnogorski Dagmar , Skiberowski Philipp , Höllmer Helge , Moritz Christian , Schulz Holger
Published: June 2023

Background

The hippocampus is a central brain structure involved in stress processing. Previous studies have linked stress-related mental disorders, such as post-traumatic stress disorder (PTSD) and major depressive disorder (MDD), with changes in hippocampus volume. As PTSD and MDD have similar symptoms, clinical diagnosis relies solely on patients reporting their cognitive and emotional experiences, leading to an interest in utilizing imaging-based data to improve accuracy. Our field study aimed to determine whether there are hippocampal subfield volume differences between stress-related mental disorders (PTSD, MDD, adjustment disorders, and AdjD) using routine clinical data from a military hospital.

Methods

Participants comprised soldiers (N = 185) with PTSD (n = 50), MDD (n = 70), PTSD with comorbid MDD (n = 38), and AdjD (n = 27). The hippocampus was segmented and volumetrized into subfields automatically using FreeSurfer. We used ANCOVA models with estimated total intracranial volume as a covariate to determine whether there were volume differences in the hippocampal subfields cornu ammonis 1 (CA1), cornu ammonis 2/3 (CA2/3), and dentate gyrus (DG) among patients with PTSD, MDD, PTSD with comorbid MDD, and AdjD. Furthermore, we added self-reported symptom duration and previous psychopharmacological and psychotherapy treatment as further covariates to examine whether there were associations with CA1, CA2/3, and DG.

Results

No significant volume differences in hippocampal subfields between stress-related mental disorders were found. No significant associations were detected between symptom duration, psychopharmacological treatment, psychotherapy, and the hippocampal subfields.

Conclusion

Hippocampal subfields may distinguish stress-related mental disorders; however, we did not observe any subfield differences. We provide several explanations for the non-results and thereby inform future field studies.
The effects of background music on English reading comprehension for English foreign language learners: evidence from an eye movement study
Authors: Su Yankui , He Meiling , Li Rongbao
Published: June 2023

Based on previous literature, the present study examines the effects of background music on English reading comprehension using eye tracking techniques. All the participants, whose first language was Chinese, were selected from a foreign language college and all of them were sophomores who majored in English. The experiment in this study was a 2 (music tempo: fast and slow) × 2 (text difficulty: difficult and easy) × 2 (background music preference: high and low) mixed design. Both musical tempo and English reading passage were within-subjects factors, and the level of music listening preference was a between-subjects factor. The results showed that the main effect of the music tempo was statistically significant, which indicated that participants read texts more quickly in the fast-tempo music condition than in the slow-tempo music condition. Furthermore, the main effect of the text difficulty was statistically significant. Additionally, the interaction between the text difficulty and music tempo was statistically significant. The music tempo had a greater effect on easy texts than on difficult texts. The results of this study reveal that it is beneficial for people who have a stronger preference for music listening to conduct English reading tasks with fast-tempo music. It is detrimental for people who have little preference for background music listening to complete difficult English reading tasks with slow-tempo music.
High prevalence of mental disorders: a population-based cross-sectional study in the city of Ilam, Iran
Authors: Kakaei Hojatollah , Maleki Farajolah , Biderafsh Azam , Valizadeh Reza , Mansournia Mohammad Ali , Pakzad Iraj , Pakzad Reza
Published: June 2023

Aim

To determine the age- and sex-standardized prevalence and risk factors of depression, anxiety, and stress symptoms in the city of Ilam.

Method

In this population-based cross-sectional study, 1,350 people were invited using a multi-stage stratified cluster random sampling method. Depression, anxiety, and stress symptoms were measured using the DASS-21 standard questionnaire. For data analysis, multiple ordinal logistic regression was used in Stata version 12 software. A significance level of 5% was considered.

Results

The data of 1,431 people were analyzed. The age- and sex-standardized prevalence (95% CI) of severe depression, anxiety, and stress symptoms was 19.90% (17.64 to 22.16), 25.95% (23.48 to 28.43), and 15.75% (13.69 to 17.81), respectively. There was a positive association among depression symptoms with female sex (OR: 1.52; p < 0.003), Kurdish ethnicity (OR: 2.15; p < 0.004), low educational level (OR: 1.37; p < 0.031), job losing history (OR: 1.64; p < 0.001), mental disorders history (OR: 2.17; p < 0.001), hopelessness for the future (OR: 5.38; p < 0.001), and history of other diseases (OR: 1.67; p < 0.001). There was a positive association among anxiety symptoms with female sex (OR: 1.72; p < 0.001), job losing history (OR: 1.53; p < 0.003), mental disorders history (OR: 2.11; p < 0.001), hopelessness to future (OR: 3.33; p < 0.001) and history of other diseases (OR: 1.97; p < 0.001). Hopelessness for the future and a history of other diseases were the most effective variables for anxiety symptoms and stress symptoms.

Conclusion

A significant proportion of Ilam’s urban population suffers from mental disorders. Increasing people’s awareness, establishing counseling centers, and improving infrastructure should be considered by mental health policymakers who work in the province.
Antibiotic resistance of Streptococcus pneumoniae in Vietnamese children with severe pneumonia: a cross-sectional study
Authors: Tran-Quang Khai , Nguyen-Thi-Dieu Thuy , Tran-Do Hung , Pham-Hung Van , Nguyen-Vu Trung , Tran-Xuan Bach , Larsson Mattias , Duong-Quy Sy
Published: June 2023

Background

Streptococcus pneumoniae is the most common bacterium that causes community-acquired pneumonia (CAP) in children. The rate of S. pneumoniae resistance to antibiotics is increasing, particularly in patients with severe CAP. Therefore, the level of antibiotic resistance of S. pneumoniae causing severe CAP in Vietnamese children requires regular monitoring.

Methods

This was a cross-sectional descriptive study. Nasopharyngeal aspiration specimens from children were cultured, isolated, and examined for S. pneumoniae. Bacterial strains were assessed for antimicrobial susceptibility, and the minimum inhibitory concentration (MIC) was determined.

Results

Eighty-nine strains of S. pneumoniae were isolated from 239 children with severe CAP. The majority of isolates were completely non-susceptible to penicillin (1.1% intermediate, 98.9% resistant) and highly resistant to erythromycin (96.6%) and clarithromycin (88.8%); the rate of resistance to ceftriaxone was 16.9%, with the proportion of intermediate resistance at 46.0%; 100% of strains were susceptible to vancomycin and linezolid. For most antibiotics, MIC50 and MIC90 were equal to the resistance threshold according to the Clinical and Laboratory Standards Institute 2021; penicillin had an eight-fold increase in MIC90 (64 mg/L) and ceftriaxone had a 1.5-fold increase in MIC90 (6 mg/L).

Conclusion

Streptococcus pneumoniae isolates described in this study were resistant to many antibiotics. Penicillin should not be the first-line antibiotic of choice, and ceftriaxone at an enhanced dose should be used instead.
Understanding human aging and the fundamental cell signaling link in age-related diseases: the middle-aging hypovascularity hypoxia hypothesis
Authors: Phua Teow J.
Published: June 2023

Aging-related hypoxia, oxidative stress, and inflammation pathophysiology are closely associated with human age-related carcinogenesis and chronic diseases. However, the connection between hypoxia and hormonal cell signaling pathways is unclear, but such human age-related comorbid diseases do coincide with the middle-aging period of declining sex hormonal signaling. This scoping review evaluates the relevant interdisciplinary evidence to assess the systems biology of function, regulation, and homeostasis in order to discern and decipher the etiology of the connection between hypoxia and hormonal signaling in human age-related comorbid diseases. The hypothesis charts the accumulating evidence to support the development of a hypoxic milieu and oxidative stress-inflammation pathophysiology in middle-aged individuals, as well as the induction of amyloidosis, autophagy, and epithelial-to-mesenchymal transition in aging-related degeneration. Taken together, this new approach and strategy can provide the clarity of concepts and patterns to determine the causes of declining vascularity hemodynamics (blood flow) and physiological oxygenation perfusion (oxygen bioavailability) in relation to oxygen homeostasis and vascularity that cause hypoxia (hypovascularity hypoxia). The middle-aging hypovascularity hypoxia hypothesis could provide the mechanistic interface connecting the endocrine, nitric oxide, and oxygen homeostasis signaling that is closely linked to the progressive conditions of degenerative hypertrophy, atrophy, fibrosis, and neoplasm. An in-depth understanding of these intrinsic biological processes of the developing middle-aged hypoxia could provide potential new strategies for time-dependent therapies in maintaining healthspan for healthy lifestyle aging, medical cost savings, and health system sustainability.
Using knowledge graphs to infer gene expression in plants
Authors: Thessen Anne E. , Cooper Laurel , Swetnam Tyson L. , Hegde Harshad , Reese Justin , Elser Justin , Jaiswal Pankaj
Published: June 2023

Introduction

Climate change is already affecting ecosystems around the world and forcing us to adapt to meet societal needs. The speed with which climate change is progressing necessitates a massive scaling up of the number of species with understood genotype-environment-phenotype (G×E×P) dynamics in order to increase ecosystem and agriculture resilience. An important part of predicting phenotype is understanding the complex gene regulatory networks present in organisms. Previous work has demonstrated that knowledge about one species can be applied to another using ontologically-supported knowledge bases that exploit homologous structures and homologous genes. These types of structures that can apply knowledge about one species to another have the potential to enable the massive scaling up that is needed throughin silicoexperimentation.

Methods

We developed one such structure, a knowledge graph (KG) using information from Planteome and the EMBL-EBI Expression Atlas that connects gene expression, molecular interactions, functions, and pathways to homology-based gene annotations. Our preliminary analysis uses data from gene expression studies inArabidopsis thalianaandPopulus trichocarpaplants exposed to drought conditions.

Results

A graph query identified 16 pairs of homologous genes in these two taxa, some of which show opposite patterns of gene expression in response to drought. As expected, analysis of the upstream cis-regulatory region of these genes revealed that homologs with similar expression behavior had conserved cis-regulatory regions and potential interaction with similar trans-elements, unlike homologs that changed their expression in opposite ways.

Discussion

This suggests that even though the homologous pairs share common ancestry and functional roles, predicting expression and phenotype through homology inference needs careful consideration of integrating cis and trans-regulatory components in the curated and inferred knowledge graph.
Implementation and assessment of a novel non-clinical skills curriculum for urology residents
Authors: Sheetz Tyler , Diab Dinah , Scimeca Alicia , Bellows Fara , Sharp David S. , Lee Cheryl T. , Posid Tasha
Published: June 2023

Background

Urology is an increasingly competitive specialty that procures a highly selected and clinically excellent cohort of residents. However, other training needs such as leadership and professional development go underrecognized despite an identified need for formal training in these areas. The aim of this study was to implement, evaluate, and pilot a non-clinical skills curriculum, a novel individualized professional development workshop series, at a single institution.

Methods

Eighteen urology residents (15/year, 3 graduates/year) participated in this study over the course of two academic years. A pre-curriculum needs assessment was completed by 15 residents in Year 1 for purposes of curriculum design. The curriculum itself was a series of 1-hour monthly workshops given by an expert speaker on topics relevant to healthcare delivery, leadership and career promotion across various contexts. Survey-based assessments tracked gains in subject knowledge and satisfaction via a pre-post test design.

Results

The pre-curriculum needs assessment indicated that trainees desired additional instruction in non-clinical skills (ps>0.1) and endorsed formal teaching to ensure success in their future careers (p<0.001). Trainees reported pre- to post-curriculum gains across each individual learning topic (Mean=20%, p<0.001) with an aggregate increase in subject knowledge of 17% for senior residents and 21% for junior residents (p<0.001).

Conclusion

A non-clinical skills curriculum implemented as a pilot ‘Hidden Curriculum’ for urology trainees was feasible and resulted in significant gains in non-clinical subject knowledge. Workshops were highly rated and trainees reported high satisfaction with the curriculum.
Biomechanics of wheelchair turning manoeuvres: novel insights into wheelchair propulsion
Authors: Chaikhot Dhissanuvach , Taylor Matthew J. D. , de Vries W. H. K. , Hettinga Florentina J.
Published: June 2023

Introduction

Wheelchair turning biomechanics is an under researched area despite its obvious relevance to functional mobility of wheelchair users. Wheelchair turns might be linked to a higher risk of upper limb injuries due to the increased forces and torques potentially associated with asymmetric movement. Our aim was to obtain a better theoretical understanding of wheelchair turning by biomechanically analyzing turns compared to steady-state straightforward propulsion (SSSFP).

Methods

Ten able-bodied men received 12-min familiarization and 10 trials (in a random order) of SSSFP and multiple left and right turns around a rectangular course. A Smartwheel was mounted at the right wheel of a standard wheelchair to measure kinetic parameters during SSSFP and of the inner hand during right turns and the outer hand during left turns. A repeated measures ANOVA was used to detect differences across tasks.

Results

Two strategies were identified: 3% demonstrated roll turns and 97% spin turns. Spin turns consisted of three phases: approach, turning and depart phase. The turning phase was accomplished by increasing peak force (72.9 ± 25.1 N vs. 43.38 ± 15.9 N in SSSFP) of the inner hand, while maintaining high push frequency of the outer hand (1.09 ± 0.20 push/s vs. 0.95 ± 0.13 push/s in SSSFP). Peak negative force and force impulse during the turning phase were much higher than SSSFP, 15.3 ± 15.7 and 4.5 ± 1.7 times higher, respectively.

Conclusion

The spin turn strategy might carry an increased risk of upper limb injuries due to higher braking force and requires particular attention by rehabilitation professionals to preserve upper limb function of long-term wheelchair users.
Productive efficiency of beef cattle production in Botswana: a latent class stochastic meta-frontier analysis
Authors: Bahta Sirak , Temoso Omphile , Ng'ombe John N. , Rich Karl M. , Baker Derek , Kaitibie Simeon , Malope Patrick
Published: June 2023

Introduction

Efficiency in food production is crucial for sustainable agriculture in developing countries. This paper contributes to the existing literature by presenting an innovative approach to modeling productive efficiency in beef cattle production. Treating farm performance across regions as unobserved heterogeneity, we determine technical efficiency of beef cattle production in Botswana. We aim to shed light on the factors influencing efficiency in this sector.

Methods

The study utilized block-level data from various annual agricultural surveys (2006–2014) covering 26 agricultural districts and six agro-ecological regions in Botswana. We employed a latent class stochastic frontier model complemented with the stochastic meta-frontier analysis.

Results

Results show that the best performing farming systems in terms of efficiency are districts with well-developed infrastructure and better access to output and input markets. In contrast, the farming systems that perform poorly consist of agricultural districts without access to livestock advisory centers, with higher average temperatures and foot and mouth disease, limiting access to export markets. The mean technical efficiency scores for beef production for agricultural districts in class one and two were 62 and 59%, respectively, implying high potential to improve beef production using the same level of agricultural inputs through efficiency-enhancing investments.

Discussion

Based on our results, it is crucial for agricultural policies to prioritize regionally specific investments that address the needs of the under-performing districts. By targeting the lagging districts, policymakers can help beef producers improve their input efficiency and bridge the technological gaps to the meta-frontier. This can be achieved through investments in infrastructure, access to livestock advisory services, and disease control measures. Such efforts will not only enhance the efficiency of beef production but also contribute to the overall sustainability of the agricultural sector in Botswana.
Bad animals, techno-fixes, and the environmental narratives of alternative protein
Authors: Hedberg Russell C.
Published: June 2023

In the last decade animal agriculture has received significant scrutiny for its many negative environmental consequences. In response to these myriad concerns a wide range of voices have advocated for diets that include less animal products (meat, dairy, eggs), often arguing that animal-based diets are inherently more resource intensive than those based on plants. Prominent in this discourse is a narratives formation developed by a slew of venture capital-backed food technology startups known as alternative protein that I refer to as the bad animal narrative. This narrative argues that livestock are fundamentally bad technology, and the solution to the many environmental problems of animal agriculture is to replace livestock with novel technologies to produce animal product alternatives that will satisfy consumer demand while also solving one of the fundamental environmental challenges of modern agriculture. In this paper I use discourse analysis frameworks from political ecology and science and technology studies to examine a large corpus of publicly available text that includes alternative protein company websites, mission statements, blogs, and connected media pieces, as well as life cycle assessment reports documenting the environmental impacts of alternative protein products as well as conventionally and alternatively produced livestock. This analysis finds that the bad animal narrative places blame on livestock without clearly providing evidence, and it rests on a set of problematic assumptions about the current food system and its possible futures. Analysis of life cycle assessment statistics finds that the industrial system, rather than livestock themselves, is the chief driver of the environmental problems of animal agriculture. The paper concludes with a consideration of the future food system envisioned by the bad animal narrative and its implications for sustainability.
Noise Cancellation Method Based on TVF-EMD with Bayesian Parameter Optimization
Authors: Yu Miaomiao , Yuan Hongyong , Li Kaiyuan , Deng Lizheng
Published: June 2023

To separate the noise and important signal features of the indoor carbon dioxide (CO2) concentration signal, we proposed a noise cancellation method, based on time-varying, filtering-based empirical mode decomposition (TVF-EMD) with Bayesian optimization (BO). The adaptive parameters of TVF-EMD, that is, bandwidth threshold ξ and B-spline order n, were determined by the BO algorithm, and the correlation coefficient for the kurtosis index (CCKur) constituted the objective function. Initially, the objective function CCKur was introduced to systematically identify anomalous signals while preserving signal feature extraction between the modes and the input signal. Subsequently, the proposed signal noise cancellation model based on TVF-EMD and the BO algorithm were employed, along with the Hurst exponent, to extract the sensitive mode. An examination of the optimization indices of the decomposed intrinsic mode functions (IMFs), namely CC, Kur, MI, EE, EEMI, and CCKur, revealed that the synthetic measurement index CCKur and objective function fitness were reasonable and effective. The proposed method exhibited better signal cancellation performance, compared to that of TVF-EMD with the default values, EMD, the moving average method, and the exponential smoothing method.
On a Class of Orthogonal Polynomials as Corrections in Lienard Differential System: Applications
Authors: Kyurkchiev Vesselin , Iliev Anton , Rahnev Asen , Kyurkchiev Nikolay
Published: June 2023

In this paper we demonstrate some specialized modules for investigating the dynamics of differential models, an integral part of a planned much more general Web-based application for scientific computing. As “corrections” in the Lienard differential system is presented a class of orthogonal polynomials (also known as “shell polynomials”). We will note that some specifics of the amplitudes of these polynomials open up the possibility of modeling signals from the field of antenna-feeder techniques. Algorithms and modules have been consistently used for: automatic generation of a theorem on the number and type of limit cycles (in the light of Melnikov’s considerations); study of the Hamiltonian of the system and “level curves”; for the study of catastrophic surfaces (in the light of Zeeman’s considerations), etc. Similar studies have been carried out for associated polynomials. Numerical examples, illustrating our results using CAS MATHEMATICA are given.
Effect of Rootstock on Vineyard Establishment Using Green-Growing Benchgrafts
Authors: Munoz Jose R. , Stauch Samantha J. , Wootten Jennifer , Kitchen Matthew , Abreu Matteo , Rodriguez Christian Jose , Casassa L. Federico , Wolpert James A. , Dodson Peterson Jean Catherine
Published: June 2023

Demand for vine plant material has increased drastically due to the ongoing expansion of viticulture, and recent widespread replanting efforts. Nurseries and growers are turning to green-grafted vines to meet demand. Unfortunately, most vineyard establishment studies have centered around dormant benchgrafted vines. Thus, little is known regarding the specific establishment trends of green-growing benchgrafts. This study aimed to explore the role rootstock selection has in green-growing benchgraft establishment and development over the first four years post-planting. Vitis vinifera L. cv. Sauvignon blanc was grafted onto multiple rootstocks of varying parentage, including ‘101-14MGT’ (V. riparia × V. rupestris), ‘1103P’ (V. berlandieri × V. rupestris), ‘110R’ (V. berlandieri × V. rupestris), ‘420A MGT’ (V. berlandieri × V. riparia), and ‘Teleki 5C’ (V. berlandieri × V. riparia). The experimental site was organized using a completely randomized design (n = 12) with all vines managed to industry-standard cultural practices. Vines grafted onto 1103P had the largest average trunk diameter (p = 0.0012) and circumference (p < 0.0001) at 22.2 mm and 7.57 cm, respectively. Vines grafted onto 110R had the second-largest trunk circumference at 6.65 cm. Vines grafted onto 110R had the largest concentration of total non-structural carbohydrates at planting at 1.47 g/L, followed by 1103P at 1.25 g/L (p < 0.0001). Total non-structural carbohydrate concentrations in the trunk during dormancy was the highest in 110R at 16.0% total dry weight (p = 0.0008). The larger trunk size and more extensive carbohydrate reserves suggest that green-growing benchgrafts using 110R or 1103P have a higher capacity and likelihood of establishment success.
Chemical Fertilization Alters Soil Carbon in Paddy Soil through the Interaction of Labile Organic Carbon and Phosphorus Fractions
Authors: Aumtong Suphathida , Chotamonsak Chakrit , Pongwongkam Paweenuch , Cantiya Kanchana
Published: June 2023

The influence of long-term chemical fertilization in paddy soils is based on the interaction between labile carbon and phosphorus fractions and the manner in which this influences soil organic carbon (SOC). Four soil depths (0–30 cm) were analyzed in this study. Easily oxidized organic carbon components, such as permanganate oxidized carbon (POXC) and dissolved organic carbon (DOC), and other physicochemical soil factors were evaluated. The correlation and principal component analyses were used to examine the relationship between soil depth and the parameter dataset. The results showed that Fe-P concentrations were greater in the 0–5 cm soil layer. DOC, inorganic phosphate fraction, and other soil physiochemical characteristics interacted more strongly with SOC in the 0–5 cm soil layer, compared to interactions in the 10–15 cm layer, influencing soil acidity. An increase in DOC in the 0–5 cm soil layer had a considerable effect on lowering SOC, consistent with P being positively correlated with POXC, but negatively with SOC and water-soluble carbon (WSC). The changes in SOC could be attributed to the relationship between DOC and inorganic phosphate fractions (such as Fe-P) under specific soil pH conditions. An increase in soil DOC could be caused by changes in the P fraction and pH. The DOC:Avai. P ratio could serve as a compromise for the C and P dynamic indicators. The soil depth interval is a critical element that influences these interactions. Agricultural policy and decision-making may be influenced by the P from chemical fertilization practices, considering the yields and environmental effects.
Is Diversified Crop Rotation an Effective Non-Chemical Strategy for Protecting Triticale Yield and Weed Diversity?
Authors: Jastrzębska Magdalena , Kostrzewska Marta K. , Marks Marek
Published: June 2023

Diversified crop rotation (DCR) has re-gained attention worldwide as a non-chemical practice for increasing the sustainability of cereal production systems. This study focused on comparing the effects of two weed management strategies, DCR (the six-field system: potato–oat–fiber flax–winter rye–faba bean–winter triticale) without the application of a herbicide (DCR strategy) and with the application of a herbicide (DCR + H strategy) on the yield of winter triticale and on the biomass and species, taxonomic, and functional diversities of weed communities. In addition, the responses of two triticale cultivars, Trapero and Borowik, to the weed management strategies were evaluated. Data from five growing seasons (2017–2021) of a long-term experiment (Bałcyny, northeastern Poland) are presented. The DCR strategy proved less effective in protecting the triticale yield than DCR + H but provided greater weed species, taxonomic and functional diversities. Borowik had a higher yield and responded to herbicide abandonment with a lower yield loss. When the DCR strategy was used, Borowik was more competitive against weeds than Trapero without reducing weed diversity. The triticale yield correlated negatively with weed biomass and diversity, and weed diversity and weed biomass were positively correlated. The findings show that winter triticale can be grown in DCR without the application of a herbicide if a high-yield, competitive cultivar such as Borowik is used. Growing such a cultivar under DCR conditions without the application of a herbicide promotes weed diversity; however, in years with less favorable weather conditions, it may be necessary to accept a moderately lower yield compared to the yield provided with herbicide protection. The relationship between weed diversity and weather conditions is a subject for further research.
Responses of Soil Microbial Communities in Soybean–Maize Rotation to Different Fertilization Treatments
Authors: Wang Yunlong , Zhang Liqiang , Meng Fangang , Lou Zixi , An Xiaoya , Jiang Xinbo , Zhao Hongyan , Zhang Wei
Published: June 2023

Rotation and fertilization are important methods used to improve crop yield. In particular, crop rotation is an effective means of enhancing ecosystem diversity; however, there exist relatively few studies regarding the effects of long-term maize–soybean rotation and fertilization on soil microbial communities. To further understand the changes in soil microbial community structure under long-term maize–soybean rotation and fertilization, we used a 9-year-old experimental site with maize–soybean rotation as the research object and soybean continuous cropping as a control. We explored the growth effects of soybean and the changes in soil microbial communities under the soybean–maize rotation system and fertilization treatments by analyzing the physicochemical properties of the soil, crop agronomic traits, yield, and changes in soil microbial community structure. The results show that, in comparison with soybean continuous cropping, the yield of soybeans was increased by 12.11% and 21.42% under maize–soybean rotation with different fertilization treatments, respectively. Additionally, there was a significant increase in the agronomic effects of nitrogen following rotation combined with fertilization. Moreover, the soil pH, SOM, and nutrient status were also improved. Bryobacter, Gemmatimonas, and Rhodanobacter were the dominant bacteria. Rotation treatment increased the relative abundance of Bryobacter and Rhodanobacter, and fertilization treatment increased the relative abundance of Gemmatimonas. Rotation also increased the stability of the bacterial community structure and strengthened the symbiotic relationship between species. The prediction of nitrogen-related functional genes indicates that rotation increased soil ammonification and nitrification. Heterocephalacria and Mrakia were the dominant fungal genera under crop rotation. The abundance of Saccharomyces Mrakia was significantly positively correlated with ammonium nitrogen levels and crop yield. Crop rotation increased the abundance of Saccharomyces Mrakia and reduced the abundance of Fusarium, but fertilization increased the abundance of Fusarium. Functional gene prediction also indicates that the relative abundance of plant pathogens was significantly reduced. This study provides a theoretical basis for soil microbial diversity and ecosystem service function in long-term soybean–maize rotation.
Ameliorative Effects of Vermicompost Application on Yield, Fertilizer Utilization, and Economic Benefits of Continuous Cropping Pepper in Karst Areas of Southwest China
Authors: Zhang Meng , Liu Yanling , Wei Quanquan , Liu Lingling , Gu Xiaofeng , Gou Jiulan , Wang Ming
Published: June 2023

In recent years, vermicompost (V) has been widely used as an amendment for improving crop productivity and soil quality. However, the ameliorative effect of vermicompost on the continuous cropping pepper remains unclear, particularly in the karst areas of southwestern China. A field experiment was conducted to study the effect of vermicompost application on the yield, quality, nutrient accumulation, fertilizer utilization, and economic benefits of continuous cropping pepper from 2021 to 2022. The experiment included six treatments: CK (no fertilizer), FP (the fertilization practice of local farmers), and FPV (FP combined with vermicompost of 1500, 2250, 3000, and 3750 kg·ha−1). The results show that vermicompost application increased the yield of fresh pod pepper by 28.34–51.36% (2021) and 47.13–68.82% (2022), whereas the yield of dry pod pepper increased by 16.97–35.14% (2021) and 34.48–62.61% (2022), respectively, compared with the FP treatment. The application of vermicompost reduced the nitrate content and increased the vitamin C (VC) and soluble sugar content of the fruits, which is beneficial for improving their quality. Vermicompost application not only increased nutrient uptake but also significantly improved agronomic efficiency (AE) and recovery efficiency (RE). In addition, although the application of vermicompost increased production costs, the increase in yield improved net incomes (16.02–31.83% in 2021 and 35.83–62.85% in 2022), especially in the FPV4 treatment. In conclusion, the use of vermicompost amendment had a positive effect on the productivity and economic benefits of continuous cropping pepper, which may be an effective nutrient management strategy for the continuous cropping pepper in the karst mountain areas of southwest China.
Zoo Animal Welfare Assessment: Where Do We Stand?
Authors: Tallo-Parra Oriol , Salas Marina , Manteca Xavier
Published: June 2023

Zoological institutions, such as zoos and aquariums, have made animal welfare a top priority, as it is not only a moral obligation but also crucial for fulfilling their roles in education and conservation. There is a need for science-based tools to assess and monitor animal welfare in these settings. However, assessing the welfare of zoo animals is challenging due to the diversity of species and lack of knowledge on their specific needs. This review aims to discuss the advantages and disadvantages of existing methodologies for assessing zoo animal welfare through: (1) A critical analysis of the main approaches to zoo animal welfare assessment; (2) A description of the most relevant animal-based welfare indicators for zoo animals with a particular focus on behavioural and physiological indicators; and (3) An identification of areas that require further research.
An Intracellular Epitope of ASFV CD2v Protein Elicits Humoral and Cellular Immune Responses
Authors: Lu Wenlong , Bai Yilin , Zhang Shuai , Zhao Xuyang , Jin Jiaxin , Zhu Xiaojing , Wang Rui , Wu Yanan , Zhang Angke , Zhang Gaiping , Zhuang Guoqing , Sun Aijun
Published: June 2023

The African swine fever virus (ASFV) causes high mortality in domestic pigs. ASFV encodes an important protein target for subunit vaccine development, CD2v, but its most effective immunological regions are not known. Herein, we generated a monoclonal antibody (mAb) named IF3 by immunizing mice against the intracellular region of the CD2v protein (CD2v-IR). 1F3 specifically recognized CD2v, which is expressed transiently in transfected Sf9 cells and also in inactivated ASFV-infected porcine alveolar macrophage (PAM) cells. The epitope recognized by 1F3 is 264EPSPREP270, which is highly conserved in ASFV genotypes. Immunization of mice with this epitope elicited an increased IgG response, including IgG1 and IgG2a subtypes, and also increased CD8+ T cells and cytokine expression. Overall, these results indicate that this epitope induces both humoral and cellular immune responses that may be used for ASFV-related subunit vaccine design and development.
The Effects of Temperature and Humidity Index on Growth Performance, Colon Microbiota, and Serum Metabolome of Ira Rabbits
Authors: Li Keyao , Abdelsattar Mahmoud M. , Gu Mingming , Zhao Wei , Liu Haoyu , Li Yafei , Guo Pingting , Huang Caiyun , Fang Shaoming , Gan Qianfu
Published: June 2023

This study investigates the effects of different THI values on growth performance, intestinal microbes, and serum metabolism in meat rabbits. The results showed that there were significant differences in THI in different location regions of the rabbit house. The high-THI group (HG) could significantly reduce average daily gain and average daily feed intake in Ira rabbits (p < 0.05). The low-THI group (LG) significantly increased the relative abundance of Blautia (p < 0.05). The HG significantly increased the relative abundance of Lachnospiraceae NK4A136 group and reduced bacterial community interaction (p < 0.05). The cytokine–cytokine receptor interactions, nuclear factor kappa B signaling pathway, and toll-like receptor signaling pathway in each rabbit’s gut were activated when the THI was 26.14 (p < 0.05). Metabolic pathways such as the phenylalanine, tyrosine, and tryptophan biosynthesis and phenylalanine metabolisms were activated when the THI was 27.25 (p < 0.05). Meanwhile, the TRPV3 and NGF genes that were associated with heat sensitivity were significantly upregulated (p < 0.05). In addition, five metabolites were found to be able to predict THI levels in the environment with an accuracy of 91.7%. In summary, a THI of 26.14 is more suitable for the growth of meat rabbits than a THI of 27.25, providing a reference for the efficient feeding of meat rabbits.
Implications of Salt Diapirism in Syn-Depositional Architecture of a Carbonate Margin-to-Edge Transition: An Example from Plataria Syncline, Ionian Zone, NW Greece
Authors: Vakalas Ioannis , Kokkalas Sotirios , Konstantopoulos Panagiotis , Tzimeas Constantinos , Kampolis Isidoros , Tsiglifi Helen , Pérez-Martin Ruben , Hernandez-Jiménez Pablo , Pita-Gutierrez Juan Pablo
Published: June 2023

The present study examines the imprint of salt tectonics on carbonate depositional patterns of the Ionian zone platform edge to slope transition. The study area is part of an overturned rim syncline adjacent to a salt diapir. The Ionian zone is made up of three distinct stratigraphic sequences (pre-, syn- and post-rift sequences) represented by evaporites and shallow water carbonates at the base that pass gradually to a sequence consisting of pelagic limestones with shale intervals. In the study area, six cross sections were constructed, mainly covering the edge-to-slope overturned succession of Early Cretaceous to Eocene carbonates (post-rift stage) in the northern limb of the syncline. In the measured sections, abrupt changes in sediment texture resulted in the formation of distinct, thick-bedded carbonate layers, identified as packstones to grainstones–floatstones, with abundant fossil fragments, indicating deposition by debrites in a platform slope or slope-toe environment. Planar and ripple cross-lamination also suggest the involvement of turbidity currents in the depositional process. In the upper levels of the Lower Cretaceous carbonates, chert bodies with irregular shapes indicate soft sediment deformation due to instability of the slope triggered by salt intrusion. Internal unconformities identified in the field and in the available seismic data combined with the vertical to overturned dipping of the strata correspond to a basal megaflap configuration. Syn-sedimentary deformation resulted in the accumulation of debritic and turbiditic layers, while the compressional regime established in the area from the Late Cretaceous to Early Eocene enhanced the fracture porosity of carbonates, which could eventually affect the reservoir properties.