bluuebunny/crossref_metadata_2025 huggingface dataset:
Reduction of Neural Machine Translation Failures by Incorporating Statistical Machine Translation
Authors: Dugonik Jani , Sepesy Maučec Mirjam , Verber Domen , Brest Janez
Published: May 2023

This paper proposes a hybrid machine translation (HMT) system that improves the quality of neural machine translation (NMT) by incorporating statistical machine translation (SMT). Therefore, two NMT systems and two SMT systems were built for the Slovenian–English language pair, each for translation in one direction. We used a multilingual language model to embed the source sentence and translations into the same vector space. From each vector, we extracted features based on the distances and similarities calculated between the source sentence and the NMT translation, and between the source sentence and the SMT translation. To select the best possible translation, we used several well-known classifiers to predict which translation system generated a better translation of the source sentence. The proposed method of combining SMT and NMT in the hybrid system is novel. Our framework is language-independent and can be applied to other languages supported by the multilingual language model. Our experiment involved empirical applications. We compared the performance of the classifiers, and the results demonstrate that our proposed HMT system achieved notable improvements in the BLEU score, with an increase of 1.5 points and 10.9 points for both translation directions, respectively.
Dynamic Analysis of Delayed Two-Species Interaction Model with Age Structure: An Application to Larch-Betula Platyphylla Forests in the Daxing’an Mountains, Northeast China
Authors: Huang Xuan , Ding Yuting , Pan Ning
Published: May 2023

Since plant–plant interaction has been the fundamental issue of the ecology community and is essential for the multispecies forest community, it is necessary to analyze the interaction mechanisms and provide suggestions for collaborative management of multispecies forests with the background of double carbon goals in China. To explore the interaction mechanisms in different interaction modes and assist China’s green development, we choose the most promising area, the Daxing’an Mountains, and its dominant species, Larch and Betula platyphylla, as research objects and establish a delayed two-species interaction model with an age structure. First, we calculate the equilibria of our model and analyze the stability of equilibria. Then, we study the existence of the Hopf bifurcation near the equilibria. Furthermore, we determine reasonable parameter values based on official data through mathematical methods, such as cluster analysis and model fitting. Finally, we carry out numerical simulations from three aspects, the evolution of a stand structure without interactions, the population dynamics in different interaction modes, and the influences of the parameters on the equilibria. Combined with simulation results, we provide biological interpretations for simulations of the stand structure evolution process and the interactions between Larch and Betula platyphylla; we also give reasonable values of the growth rates and mortalities for developing forest strategies.
Bursting Dynamics in a Singular Vector Field with Codimension Three Triple Zero Bifurcation
Authors: Lyu Weipeng , Li Shaolong , Chen Zhenyang , Bi Qinsheng
Published: May 2023

As a kind of dynamical system with a particular nonlinear structure, a multi-time scale nonlinear system is one of the essential directions of the current development of nonlinear dynamics theory. Multi-time scale nonlinear systems in practical applications are often complex forms of coupling of high-dimensional and high codimension characteristics, leading to various complex bursting oscillation behaviors and bifurcation characteristics in the system. For exploring the complex bursting dynamics caused by high codimension bifurcation, this paper considers the normal form of the vector field with triple zero bifurcation. Two kinds of codimension-2 bifurcation that may lead to complex bursting oscillations are discussed in the two-parameter plane. Based on the fast–slow analysis method, by introducing the slow variable W=Asin(ωt), the evolution process of the motion trajectory of the system changing with W was investigated, and the dynamical mechanism of several types of bursting oscillations was revealed. Finally, by varying the frequency of the slow variable, a class of chaotic bursting phenomena caused by the period-doubling cascade is deduced. Developing related work has played a positive role in deeply understanding the nature of various complex bursting phenomena and strengthening the application of basic disciplines such as mechanics and mathematics in engineering practice.
The Square of Some Generalized Hamming Graphs
Authors: Li Yipeng , Zhang Jing , Wang Meili
Published: May 2023

In this paper, we study the square of generalized Hamming graphs by the properties of abelian groups, and characterize some isomorphisms between the square of generalized Hamming graphs and the non-complete extended p-sum of complete graphs. As applications, we determine the eigenvalues of the square of some generalized Hamming graphs.
On Ulam Stability of a Partial Differential Operator in Banach Spaces
Authors: Novac Adela , Otrocol Diana , Popa Dorian
Published: May 2023

In this paper, we prove that, if infx∈A|f(x)|=m>0, then the partial differential operator D defined by D(u)=∑k=1nfk∂u∂xk−fu, where f,fi∈C(A,R),u∈C1(A,X),i=1,…,n,I⊂R is an interval, A=I×Rn−1 and X is a Banach space, is Ulam stable with the Ulam constant K=1m. Moreover, if infx∈A|f(x)|=0, we prove that D is not generally Ulam stable.
Modeling and Heuristically Solving Group Train Operation Scheduling for Heavy-Haul Railway Transportation
Authors: Chen Weiya , Zhuo Qinyu , Zhang Lu
Published: May 2023

In light of the improvements to the capacity and timeliness of heavy-haul railway transportation that can be organized through group trains originating at a technical station, we address a group train operation scheduling problem with freight demand importance via a newly proposed mixed integer programming model and a simulated annealing algorithm. The optimization objective of the mixed integer programming model is to minimize the weighted sum of the transportation cost and the total cargo travel time under the condition of matching freight supply and demand within the optimization period. The main constraints are extracted from the supply and demand relations, the cargo delivery time commitment, the maintenance time, and the number of locomotives. A simulated annealing algorithm was constructed to generate the grouping scheme, the stopping scheme and the running schedule of group trains. A numerical experiment based on a real heavy-haul railway configuration was employed to verify the efficacy of the proposed model and heuristics algorithm. The results show that the proposed methodology can achieve high-quality solutions. The case results reveal that the freight volume increased by 2.03%, the departure cost decreased by CNY 337,000, the transportation cost which results from the difference in the supply and demand matching increased by CNY 27,764, and the total cargo travel time decreased by 40.9%, indicating that group train operation can create benefits for both railway enterprises and customers.
Effect of Nitrogen, Salinity, and Light Intensity on the Biomass Composition of Nephroselmis sp.: Optimization of Lipids Accumulation (Including EPA)
Authors: Mastropetros Savvas Giannis , Tsigkou Konstantina , Cladas Yannis , Priya Arun Kumar , Kornaros Michael
Published: May 2023

Microalgal biomass is characterized by high protein, carbohydrates, and lipids concentrations. However, their qualitative and quantitative compositions depend not only on the cultivated species but also on the cultivation conditions. Focusing on the microalgae’s ability to accumulate significant fatty acids (FAs) amounts, they can be valorized either as dietary supplements or for biofuel production, depending on the accumulated biomolecules. In this study, a local isolate (Nephroselmis sp.) was precultured under autotrophic conditions, while the Box–Behnken experimental design followed using the parameters of nitrogen (0–250 mg/L), salinity (30–70 ppt) and illuminance (40–260 μmol m−2 s−1) to evaluate the accumulated biomolecules, with an emphasis on the amount of FAs and its profile. Regardless of the cultivation conditions, the FAs of C14:0, C16:0, and C18:0 were found in all samples (up to 8% w/w in total), while the unsaturated C16:1 and C18:1 were also characterized by their high accumulations. Additionally, the polyunsaturated FAs, including the valuable C20:5n3 (EPA), had accumulated when the nitrogen concentration was sufficient, and the salinity levels remained low (30 ppt). Specifically, EPA approached 30% of the total FAs. Therefore, Nephroselmis sp. could be considered as an alternative EPA source compared to the already-known species used in food supplementation.
Risk Factors for Anaphylaxis in Children Allergic to Peanuts
Authors: Petek Tadej , Lajhar Mija , Krašovec Blažka , Homšak Matjaž , Kavalar Maja , Korošec Peter , Koren Brigita , Tomazin Maja , Hojnik Tina , Berce Vojko
Published: May 2023

Background and Objectives: A peanut allergy is the most common single cause of anaphylaxis in children. The risk factors for anaphylaxis in children with a peanut allergy are not well defined. Therefore, we aimed to identify epidemiological, clinical, and laboratory characteristics of children with a peanut allergy that may predict the severity of the allergic reaction and anaphylaxis. Materials and Methods: We conducted a cross-sectional study and included 94 children with a peanut allergy. Allergy testing was performed, including skin prick testing and the determination of specific IgE levels to peanuts and their Ara h2 component. In case of discordance between patient history and allergy testing, an oral food challenge with peanuts was performed. Results: Anaphylaxis and moderate and mild reactions to peanuts occurred in 33 (35.1%), 30 (31.9%), and 31 (33.0%) patients, respectively. The severity of the allergic reaction was only weakly correlated (p = 0.04) with the amount of peanuts consumed. The median number of allergic reactions to peanuts was 2 in children with anaphylaxis compared to 1 in other patients (p = 0.04). The median level of specific IgE to Ara h2 was 5.3 IU/mL in children with anaphylaxis compared to 0.6 IU/mL and 10.3 IU/mL in children with mild and moderate peanut allergies (p = 0.06). The optimal cutoff for distinguishing between anaphylaxis and a less severe allergic reaction to peanuts was a specific IgE Ara h2 level of 0.92 IU/mL with 90% sensitivity and 47.5% specificity for predicting anaphylaxis (p = 0.04). Conclusions: Epidemiological and clinical characteristics of the patient cannot predict the severity of the allergic reaction to peanuts in children. Even standard allergy testing, including component diagnostics, is a relatively poor predictor of the severity of an allergic reaction to peanuts. Therefore, more accurate predictive models, including new diagnostic tools, are needed to reduce the need for oral food challenge in most patients.
Laser Treatment Monitoring with Reflectance Confocal Microscopy
Authors: Guida Stefania , Longo Caterina , Amato Simone , Rossi Anthony M. , Manfredini Marco , Ciardo Silvana , Spadafora Marco , Nisticò Steven P. , Mercuri Santo R. , Rongioletti Franco , Zerbinati Nicola , Pellacani Giovanni
Published: May 2023

Laser treatments have become popular in Dermatology. In parallel to technologic development enabling the availability of different laser wavelengths, non-invasive skin imaging techniques, such as reflectance confocal microscopy (RCM), have been used to explore morphologic and qualitative skin characteristics. Specifically, RCM can be applied to cosmetically sensitive skin areas such as the face, without the need for skin biopsies. For these reasons, apart from its current use in skin cancer diagnosis, our systematic review reveals how RCM can be employed in the field of laser treatment monitoring, being particularly suitable for the evaluation of variations in epidermis and dermis, and pigmentary and vascular characteristics of the skin. This systematic review article aims to provide an overview on current applications of RCM laser treatment monitoring, while describing RCM features identified for different applications. Studies on human subjects treated with laser treatments, monitored with RCM, were included in the current systematic review. Five groups of treatments were identified and described: skin rejuvenation, scar tissue, pigmentary disorders, vascular disorders and other. Interestingly, RCM can assist treatments with lasers targeting all chromophores in the skin and exploiting laser induced optical breakdown. Treatment monitoring encompasses assessment at baseline and examination of changes after treatment, therefore revealing details in morphologic alterations underlying different skin conditions and mechanisms of actions of laser therapy, as well as objectify results after treatment.
Lower Extremity Muscle Activation during the Star Excursion Balance Test in Patients with Chronic Ankle Instability and Copers
Authors: Kwon Yong Ung
Published: May 2023

Background and Objectives: The aim of this study was to assess the impact of ankle muscles on performance of the Star Excursion Balance Test (SEBT) among individuals with stable ankles, a history of ankle sprain, and chronic ankle instability (CAI). Materials and Methods: Sixty subjects (twenty per group) performed the SEBT in each of the anterior (A), posteromedial (PM), and posterolateral (PL) directions. Normalized maximum reach distance (NMRD) and normalized mean amplitude of the tibialis anterior (NMA_TA), fibularis longus (NMA_FL), and medial gastrocnemius (NMA_MG) were measured during performance of the SEBT. Results: Copers have greater NMRD than subjects with stable ankles and those with CAI, and subjects with stable ankles also have greater NMRD than those with CAI in only the PL direction. Subjects with stable ankles and those with CAI showed greater NMA_TA than copers. The A direction showed greater NMA_TA than the PM and PL directions. Copers showed greater NMA_FL than subjects with stable ankles. Subjects with CAI showed greater NMA_MG than copers and subjects with stable ankles. The A and PL directions showed greater NMA_MG than the PM direction. Conclusions: Overall, copers and/or subjects with CAI demonstrated altered neuromuscular function by compensating for their ankle muscles when compared to subjects with stable ankles due to a history of ankle sprain.
Highlights on the Development, Related Patents, and Prospects of Lenacapavir: The First-in-Class HIV-1 Capsid Inhibitor for the Treatment of Multi-Drug-Resistant HIV-1 Infection
Authors: Dzinamarira Tafadzwa , Almehmadi Mazen , Alsaiari Ahad Amer , Allahyani Mamdouh , Aljuaid Abdulelah , Alsharif Abdulaziz , Khan Abida , Kamal Mehnaz , Rabaan Ali A. , Alfaraj Amal H. , AlShehail Bashayer M. , Alotaibi Nouf , AlShehail Shams M. , Imran Mohd
Published: May 2023

The multidrug-resistant (MDR) human immunodeficiency virus 1 (HIV-1) infection is an unmet medical need. HIV-1 capsid plays an important role at different stages of the HIV-1 replication cycle and is an attractive drug target for developing therapies against MDR HIV-1 infection. Lenacapavir (LEN) is the first-in-class HIV-1 capsid inhibitor approved by the USFDA, EMA, and Health Canada for treating MDR HIV-1 infection. This article highlights the development, pharmaceutical aspects, clinical studies, patent literature, and future directions on LEN-based therapies. The literature for this review was collected from PubMed, authentic websites (USFDA, EMA, Health Canada, Gilead, and NIH), and the free patent database (Espacenet, USPTO, and Patent scope). LEN has been developed by Gilead and is marketed as Sunlenca (tablet and subcutaneous injection). The long-acting and patient-compliant LEN demonstrated a low level of drug-related mutations, is active against MDR HIV-1 infection, and does not reveal cross-resistance to other anti-HIV drugs. LEN is also an excellent drug for patients having difficult or limited access to healthcare facilities. The literature has established additive/synergistic effects of combining LEN with rilpivirine, cabotegravir, islatravir, bictegravir, and tenofovir. HIV-1 infection may be accompanied by opportunistic infections such as tuberculosis (TB). The associated diseases make HIV treatment complex and warrant drug interaction studies (drug–drug, drug–food, and drug–disease interaction). Many inventions on different aspects of LEN have been claimed in patent literature. However, there is a great scope for developing more inventions related to the drug combination of LEN with anti-HIV/anti-TB drugs in a single dosage form, new formulations, and methods of treating HIV and TB co-infection. Additional research may provide more LEN-based treatments with favorable pharmacokinetic parameters for MDR HIV-1 infections and associated opportunistic infections such as TB.
Experimental Study on Mechanical Properties of Different Resins Used in Oral Environments
Authors: Baciu Elena-Raluca , Savin Carmen Nicoleta , Tatarciuc Monica , Mârțu Ioana , Butnaru Oana Maria , Aungurencei Andra Elena , Mihalache Andrei-Marius , Diaconu-Popa Diana
Published: May 2023

Background and Objectives: Acrylic resins remain the materials of choice for removable prosthesis due to their indisputable qualities. The continuous evolution in the field of dental materials offers practitioners today a multitude of therapeutic options. With the development of digital technologies, including both subtractive and additive methods, workflow has been considerably reduced and the precision of prosthetic devices has increased. The superiority of prostheses made by digital methods compared to conventional prostheses is much debated in the literature. Our study’s objective was to compare the mechanical and surface properties of three types of resins used in conventional, subtractive, and additive technologies and to determine the optimal material and the most appropriate technology to obtain removable dentures with the highest mechanical longevity over time. Materials and Methods: For the mechanical tests, 90 samples were fabricated using the conventional method (heat curing), CAD/CAM milling, and 3D printing technology. The samples were analyzed for hardness, roughness, and tensile tests, and the data were statistically compared using Stata 16.1 software (StataCorp, College Station, TX, USA). A finite element method was used to show the behavior of the experimental samples in terms of the crack shape and its direction of propagation. For this assessment the materials had to be designed inside simulation software that has similar mechanical properties to those used for obtaining specimens for tensile tests. Results: The results of this study suggested that CAD/CAM milled samples showed superior surface characteristics and mechanical properties, comparable with conventional heat-cured resin samples. The propagation direction predicted by the finite element analysis (FEA) software was similar to that observed in a real-life specimen subjected to a tensile test. Conclusions: Removable dentures made from heat-cured resins remain a clinically acceptable option due to their surface quality, mechanical properties, and affordability. Three-dimensional printing technology can be successfully used as a provisional or emergency therapeutic solution. CAD/CAM milled resins exhibit the best mechanical properties with great surface finishes compared to the other two processing methods.
Short-Term Effect of Conventional Versus Accelerated Corneal Cross-Linking Protocol on Corneal Geography and Stability
Authors: Vidas Pauk Sania , Jandroković Sonja , Lešin Gaćina Dina , Tomić Martina , Bulum Tomislav , Pupić Bakrač Ana , Kuzman Tomislav , Knežević Josip , Kalauz Miro
Published: May 2023

Purpose: To determine the 6-month effect of conventional (CXL30) and accelerated cross-linking with a UVA intensity of 9 mW/cm2 (CXL10) on corneal stability and to investigate whether there was a difference in ABCD grading system parameters regarding the two different procedures. Methods: Twenty-eight eyes of 28 patients with a documented keratoconus (KN) progression were included. Patients were selected to undergo either epi off CXL30 or CXL10. At the baseline and the follow-up visits after one (V1), three (V2), and six months (V3), the patients underwent complete ophthalmic examination and corneal tomography. Results: In the CXL30 group, all the parameters from the ABCD grading system significantly changed from baseline to V3; parameter A decreased (p = 0.048), B and C increased (p = 0.010, p < 0.001), and D decreased (p < 0.001). In the CXL10 group, there were no changes in parameters A (p = 0.247) and B (p = 0.933), though parameter C increased (p = 0.001) and D decreased (p < 0.001). After an initial decline after one month, visual acuity (VA) recovered on V2 and V3 (p < 0.001), and median maximal keratometry (Kmax) decreased in both groups (p = 0.001, p = 0.035). In the CXL30 group, there were significant changes in other parameters; average pachymetric progression index (p < 0.001), Ambrósio relational thickness maximum (ARTmax) (p = 0.008), front and back mean keratometry (p < 0.001), pachymetry apex (PA) (p < 0.001), and front elevation (p = 0.042). However, in the CXL10 group, there were significant changes only in ARTmax (p = 0.019) and PA (p < 0.001). Conclusion: Both epi-off CXL protocols showed similar short-term efficacy in improving VA and Kmax, halting the progression of KN, and both similarly changed tomographic parameters. However, the conventional protocol modified the cornea more significantly.
Effect of Roselle Flower Extract (Hibiscus sabdariffa Linn.) on Reducing Steatosis and Steatohepatitis in Vitamin B12 Deficiency Rat Model
Authors: Ujianti Irena , Sianipar Imelda Rosalyn , Prijanti Ani Retno , Hasan Irsan , Arozal Wawaimuli , Jusuf Ahmad Aulia , Wibowo Heri , Prihartono Joedo , Amani Patwa , Santoso Dewi Irawati Soeria
Published: May 2023

Background and Objectives: Non-alcoholic Fatty Liver Disease (NAFLD) can occur as a result of micronutrient deficiencies. Hibiscus sabdarifa, a plant used in traditional medicine, contains ingredients that can help prevent this process. This study looked at the potency of Hibiscus sabdariffa Ethanol Extract (HSE) to prevent homocysteine-induced liver damage in animals that were deficient in vitamin B12. Materials and Methods: A comparative study of the effects of roselle extract is presented in an experimental design. Thirty Sprague–Dawley rats were divided into six groups using randomization. To demonstrate the absence of liver damage in the experimental animals under normal conditions, a control group was fed a normal diet without HSE. For the induction of liver damage in the experimental animals, the vitamin B12-restricted group was administered a vitamin B12-restricted diet. To test the effect of HSE on liver damage, the treatment group was given HSE along with a vitamin B12-restricted diet. Each group was given two treatment periods of eight and sixteen weeks. These results were compared with the results of the parameter examination between the vitamin B12 restriction group, with and without HSE, using an ANOVA statistic. The data were analyzed with licensed SPSS 20.0 software. Results: HSE significantly increased the blood levels of vitamin B12 while lowering homocysteine levels. The administration of HSE reduced liver damage based on the activity of liver function enzymes in the plasma due to a limitation of vitamin B12. HSE decreased Sterol Regulatory Element-Binding Protein-1c (SREBP1c) and Nuclear Factor Kappa B (NFkB) protein expressions in the liver tissue, but did not decrease Glucose-Regulated Protein 78 (GRP78) protein expression. Significantly, the levels of Tumor Necrosis Factor alpha (TNF-a) and IL-6 in the liver tissue were lower, while the levels of IL-10 and Nuclear factor-erythroid-2 Related Factor 2 (NRF2) were higher with HSE administration. HSE produced a better histopathological profile of the Hematoxylin and Eosin (H&E)–Masson tricrome for inflammation, fat and fibrosis in the liver. Conclusions: In this study, HSE was found to slow the development of liver damage in experimental animals that were given a vitamin B12-deficient diet.
The Interplay between Microbiota and Chemotherapy-Derived Metabolites in Breast Cancer
Authors: Plaza-Diaz Julio , Álvarez-Mercado Ana Isabel
Published: May 2023

The most common cancer in women is breast cancer, which is also the second leading cause of death in this group. It is, however, important to note that some women will develop or will not develop breast cancer regardless of whether certain known risk factors are present. On the other hand, certain compounds are produced by bacteria in the gut, such as short-chain fatty acids, secondary bile acids, and other metabolites that may be linked to breast cancer development and mediate the chemotherapy response. Modeling the microbiota through dietary intervention and identifying metabolites directly associated with breast cancer and its complications may be useful to identify actionable targets and improve the effect of antiangiogenic therapies. Metabolomics is therefore a complementary approach to metagenomics for this purpose. As a result of the combination of both techniques, a better understanding of molecular biology and oncogenesis can be obtained. This article reviews recent literature about the influence of bacterial metabolites and chemotherapy metabolites in breast cancer patients, as well as the influence of diet.
Connection of ssDNA to Silicon Substrate Based on a Mechano–Chemical Method
Authors: Shi Liqiu , Yu Feng , Ding Mingming , Hang Zhouming , Feng Yan , Yan Aifang , Dong Hongji
Published: May 2023

A novel fabrication process to connect single-stranded DNA (ssDNA)to a silicon substrate based on a mechano–chemical method is proposed. In this method, the single crystal silicon substrate was mechanically scribed in a diazonium solution of benzoic acid using a diamond tip which formed silicon free radicals. These combined covalently with organic molecules of diazonium benzoic acid contained in the solution to form self-assembled films (SAMs). The SAMs were characterized and analyzed by AFM, X-ray photoelectron spectroscopy and infrared spectroscopy. The results showed that the self-assembled films were covalently connected to the silicon substrate by Si–C. In this way, a nano-level benzoic acid coupling layer was self-assembled on the scribed area of the silicon substrate. The ssDNA was further covalently connected to the silicon surface by the coupling layer. Fluorescence microscopy showed that ssDNA had been connected, and the influence of ssDNA concentration on the fixation effect was studied. The fluorescence brightness gradually increased with the gradual increase in ssDNA concentration from 5 μmol/L to 15 μmol/L, indicating that the fixed amount of ssDNA increased. However, when the concentration of ssDNA increased from 15 μmol/L to 20 μmol/L, the detected fluorescence brightness decreased, indicating that the hybridization amount decreased. The reason may be related to the spatial arrangement of DNA and the electrostatic repulsion between DNA molecules. It was also found that ssDNA junctions on the silicon surface were not very uniform, which was related to many factors, such as the inhomogeneity of the self-assembled coupling layer, the multi-step experimental operation and the pH value of the fixation solution.
Field Effect Transistor with Nanoporous Gold Electrode
Authors: Bakhoum Ezzat G. , Zhang Cheng
Published: May 2023

Nanoporous gold (NPG) has excellent catalytic activity and has been used in the recent literature on this issue as a sensor in various electrochemical and bioelectrochemical reactions. This paper reports on a new type of metal–oxide–semiconductor field-effect transistor (MOSFET) that utilizes NPG as a gate electrode. Both n-channel and p-channel MOSFETs with NPG gate electrodes have been fabricated. The MOSFETs can be used as sensors and the results of two experiments are reported: the detection of glucose and the detection of carbon monoxide. A detailed comparison of the performance of the new MOSFET to that of the older generation of MOSFETs fitted with zinc oxide gate electrodes is given.
Disturbance Characteristics of 1T DRAM Arrays Consisting of Feedback Field-Effect Transistors
Authors: Jeon Juhee , Cho Kyoungah , Kim Sangsig
Published: May 2023

Challenges in scaling dynamic random-access memory (DRAM) have become a crucial problem for implementing high-density and high-performance memory devices. Feedback field-effect transistors (FBFETs) have great potential to overcome the scaling challenges because of their one-transistor (1T) memory behaviors with a capacitorless structure. Although FBFETs have been studied as 1T memory devices, the reliability in an array must be evaluated. Cell reliability is closely related to device malfunction. Hence, in this study, we propose a 1T DRAM consisting of an FBFET with a p+–n–p–n+ silicon nanowire and investigate the memory operation and disturbance in a 3 × 3 array structure through mixed-mode simulations. The 1T DRAM exhibits a write speed of 2.5 ns, a sense margin of 90 μA/μm, and a retention time of approximately 1 s. Moreover, the energy consumption is 5.0 × 10−15 J/bit for the write ‘1’ operation and 0 J/bit for the hold operation. Furthermore, the 1T DRAM shows nondestructive read characteristics, reliable 3 × 3 array operation without any write disturbance, and feasibility in a massive array with an access time of a few nanoseconds.
A Double-Layer Dual-Polarized Huygens Metasurface and Its Meta-Lens Antenna Applications
Authors: Cao Shuo , Zhou Jianhe , Li Ruxue , Xue Chunhua
Published: May 2023

In this paper, a dual-polarized Huygens unit is proposed, which has a double-layer metallic pattern etched on both sides of one dielectric substrate. Induced magnetism enables the structure to support Huygens’ resonance, thus obtaining nearly complete available transmission phase coverage. By optimizing the structural parameters, a better transmission performance can be achieved. When the Huygens metasurface was used for the design of a meta-lens, good radiation performance was exhibited, with a maximum gain of 31.15 dBi at 28 GHz, an aperture efficiency of 42.7% and a 3 dB gain bandwidth of 26.4 GHz to 30 GHz (12.86%). Due to its excellent radiation performance and very simple fabrication, this Huygens meta-lens has important applications in millimeter-wave communication systems.
Surface Quality and Material Removal Rate in Fabricating Microtexture on Tungsten Carbide via Femtosecond Laser
Authors: Li Guangxian , Li Xuanang , He Guichao , Fan Ruiguang , Li Feiyuan , Ding Songlin
Published: May 2023

Tungsten carbide is currently the most widely used tool material for machining difficult-to-machine materials, such as titanium alloys and nickel-based super alloys. In order to improve the performance of tungsten carbide tools, surface microtexturing, a novel technology that can effectively reduce cutting forces and cutting temperatures and improve wear resistance, has been applied in metalworking processes. However, when fabricating the micro-textures such as micro-grooves or micro-holes on tool surfaces, the significant decrease in material removal rate is a major obstacle. In this study, a straight-groove-array microtexture was fabricated on the surface of tungsten carbide tools via a femtosecond laser with different machining parameters including laser power, laser frequency, and scanning speed. The material removal rate, surface roughness, and the laser-induced periodic surface structure were analyzed. It was found that the increase in the scanning speed decreased the material removal rate, whereas increasing the laser power and laser frequency had the opposite effects on the material removal rate. The laser-induced periodic surface structure was found to have a significant influence on the material removal rate, and the destruction of the laser-induced periodic surface structure was the reason for the reduction in the material removal rate. The results of the study revealed the fundamental mechanisms of the efficient machining method for the fabrication of microtextures on ultrahard materials with an ultrashort laser.
Public Health Importance of Preventive Measures for Salmonella Tennessee and Salmonella Typhimurium Strain LT2 Biofilms
Authors: Asefaw Simen , Aras Sadiye , Kabir Md Niamul , Wadood Sabrina , Chowdhury Shahid , Fouladkhah Aliyar Cyrus
Published: May 2023

Various serovars of Salmonella had been the subject of research for over 150 years; nonetheless, the bacterium has remained an important pathogen of public health concern to date. The tremendous ability of Salmonella to form biofilms on biotic and abiotic surfaces is an important underlying reason for the prevalence of this opportunistic pathogen in healthcare, manufacturing, and the food chain. The current study illustrates that using very common industrial antimicrobial treatments at the highest concentrations suggested by the manufacturers is only efficacious against planktonic and one-day mature biofilms of the pathogen while exhibiting a lack of efficacy for complete removal of bacterial biofilms formed for longer than 2 days. This exhibits the importance of preventive measures against Salmonella biofilm formation in healthcare and manufacturing facilities, schools, nursing homes, and domestic environments. Additionally, our study illustrates the importance of including both planktonic and sessile cells of the pathogen in microbiology validation studies, especially for niche and hard-to-reach surfaces. The current study additionally investigated the suitability of an avirulent strain of the pathogen as a surrogate for pathogenic Salmonella serovars for public health microbiology validation studies when the use of virulent strains is not economically feasible or not possible due to safety concerns.
Screening of New Industrially Important Bacterial Strains for 1,3-Propanediol, 2,3-Butanediol and Ethanol Production through Biodiesel-Derived Glycerol Fermentations
Authors: Karayannis Dimitris , Vasilakis Gabriel , Charisteidis Ioannis , Litinas Alexandros , Manolopoulou Eugenia , Tsakalidou Effie , Papanikolaou Seraphim
Published: May 2023

A study on the ability of new microbial strains to assimilate biodiesel-derived glycerol at low purity (75% w/w) and produce extra-cellular platform chemical compounds of major interest was carried out. After screening several bacterial strains under different fermentation conditions (e.g., pH, O2 availability, glycerol purity), three of the screened strains stood out for their high potential to produce valued-added products such as 2,3-butanediol (BDO), 1,3-propanediol (PDO) and ethanol (EtOH). The results indicate that under aerobic conditions, Klebsiella oxytoca ACA-DC 1581 produced BDO in high yield (YBDO/Gly = 0.46 g/g, corresponding to 94% of the maximum theoretical yield; Ymt) and titer, while under anaerobic conditions, Citrobacter freundii NRRL-B 2645 and Enterobacter ludwigii FMCC-204 produced PDO (YPDO/Gly = 0.56 g/g, 93% of Ymt) and EtOH (YEtOH/Gly = 0.44 g/g, 88% of Ymt), respectively. In the case of C. freundii, the regulation of pH proved to be mandatory, due to lactic acid production and a subsequent drop of pH that resulted in fermentation ceasing. In the fed-batch culture of K. oxytoca, the BDO maximum titer reached almost 70 g/L, the YBDO/Gly and the mean productivity value (PrBDO) were 0.47 g/g and 0.4 g/L/h, respectively, while no optimization was imposed. The final BDO production obtained by this wild strain (K. oxytoca) is among the highest in the international literature, although the bioprocess requires optimization in terms of productivity and total cost. In addition, for the first time in the literature, a strain from the species Hafnia alvei (viz., Hafnia alvei ACA-DC 1196) was reported as a potential BDO producer. The strains as well as the methodology proposed in this study can contribute to the development of a biorefinery that complements the manufacture of biofuels with high-value biobased chemicals.
Genomic Characterization of Arcobacter butzleri Strains Isolated from Various Sources in Lithuania
Authors: Uljanovas Dainius , Gölz Greta , Fleischmann Susanne , Kudirkiene Egle , Kasetiene Neringa , Grineviciene Audrone , Tamuleviciene Egle , Aksomaitiene Jurgita , Alter Thomas , Malakauskas Mindaugas
Published: May 2023

Arcobacter (A.) butzleri, the most widespread species within the genus Arcobacter, is considered as an emerging pathogen causing gastroenteritis in humans. Here, we performed a comparative genome-wide analysis of 40 A. butzleri strains from Lithuania to determine the genetic relationship, pangenome structure, putative virulence, and potential antimicrobial- and heavy-metal-resistance genes. Core genome single nucleotide polymorphism (cgSNP) analysis revealed low within-group variability (≤4 SNPs) between three milk strains (RCM42, RCM65, RCM80) and one human strain (H19). Regardless of the type of input (i.e., cgSNPs, accessory genome, virulome, resistome), these strains showed a recurrent phylogenetic and hierarchical grouping pattern. A. butzleri demonstrated a relatively large and highly variable accessory genome (comprising of 6284 genes with around 50% of them identified as singletons) that only partially correlated to the isolation source. Downstream analysis of the genomes resulted in the detection of 115 putative antimicrobial- and heavy-metal-resistance genes and 136 potential virulence factors that are associated with the induction of infection in host (e.g., cadF, degP, iamA), survival and environmental adaptation (e.g., flagellar genes, CheA-CheY chemotaxis system, urease cluster). This study provides additional knowledge for a better A. butzleri-related risk assessment and highlights the need for further genomic epidemiology studies in Lithuania and other countries.
Structural Identification and Antioxidant Activity of Loach Protein Enzymatic Hydrolysates
Authors: Mao Jinrong , Li Shunqin , Yun Liyuan , Zhang Min
Published: May 2023

Loach, rich in nutrients, such as proteins, amino acids, and mineral elements, is being gradually favored by consumers. Therefore, in this study, the antioxidant activity and structural characteristics of loach peptides were comprehensively analyzed. The loach protein (LAP) with a molecular weight between 150 and 3000 Da was graded by ultrafiltration and nanofiltration processes, which exhibited excellent scavenging activity against DPPH radical (IC50 2.91 ± 0.02 mg/mL), hydroxyl radical (IC50 9.95 ± 0.03 mg/mL), and superoxide anion radical (IC50 13.67 ± 0.33 mg/mL). Additionally, LAP was purified by gel filtration chromatography, and two principal components (named as LAP-I and LAP-II) were isolated. A total of 582 and 672 peptides were identified in LAP-I and LAP-II, respectively, through structural analysis. The XRD results revealed that LAP-I and LAP-II had an irregular amorphous structure. The 2D-NMR spectroscopy results suggested that LAP-I had a compact stretch conformation in the D2O solution, while LAP-II had a folded conformation. Overall, the study results suggested that loach peptide could be a potential antioxidant agent and might provide valuable information for chain conformation and antioxidant mechanism research further.
Gut Protective Effect from D-Methionine or Butyric Acid against DSS and Carrageenan-Induced Ulcerative Colitis
Authors: Ikeda Yuka , Matsuda Satoru
Published: May 2023

Microbiome dysbiosis resulting in altered metabolite profiles may be associated with certain diseases, including inflammatory bowel diseases (IBD), which are characterized by active intestinal inflammation. Several studies have indicated the beneficial anti-inflammatory effect of metabolites from gut microbiota, such as short-chain fatty acids (SCFAs) and/or D-amino acids in IBD therapy, through orally administered dietary supplements. In the present study, the potential gut protective effects of d-methionine (D-Met) and/or butyric acid (BA) have been investigated in an IBD mouse model. We have also built an IBD mouse model, which was cost-effectively induced with low molecular weight DSS and kappa-carrageenan. Our findings revealed that D-Met and/or BA supplementation resulted in the attenuation of the disease condition as well as the suppression of several inflammation-related gene expressions in the IBD mouse model. The data shown here may suggest a promising therapeutic potential for improving symptoms of gut inflammation with an impact on IBD therapy. However, molecular metabolisms need to be further explored.
Endogenous n-Alkanes in Vegetable Oils: Validation of a Rapid Offline SPE-GC-FID Method, Comparison with Online LC-GC-FID and Potential for Olive Oil Quality Control
Authors: Srbinovska Ana , Lucci Paolo , Conchione Chiara , Barp Laura , Moret Sabrina
Published: May 2023

The potential of endogenous n-alkane profiling for the assessment of extra virgin olive oils (EVOO) adulteration (blends with cheaper vegetable oils) has been studied by relatively few authors. Analytical methods used for this purpose often involve tedious and solvent-intensive sample preparation prior to analytical determination, making them unattractive. A rapid and solvent-sparing offline solid phase extraction (SPE) gas chromatography (GC) flame ionization detection (FID) method for the determination of endogenous n-alkanes in vegetable oils was, therefore, optimized and validated. The optimized method demonstrated good performance characteristics in terms of linearity (R2 > 0.999), recovery (on average 94%), and repeatability (residual standard deviation, RSD < 11.9%). The results were comparable to those obtained with online high-performance liquid chromatography (HPLC)-GC- FID ( RSD < 5.1%). As an example of an application to prove the potentiality of endogenous n-alkanes in revealing frauds, the data set obtained from 16 EVOO, 9 avocado oils (AVO), and 13 sunflower oils (SFO), purchased from the market, was subjected to statistical analysis and principal component analysis. Two powerful indices, namely (n-C29 + n-C31)/(n-C25 + n-C26) and n-C29/n-C25, were found to reveal the addition of 2% SFO in EVOO and 5% AVO in EVOO, respectively. Further studies are needed to confirm the validity of these promising indices.
Antioxidant and Anti-Inflammatory Activity on LPS-Stimulated RAW 264.7 Macrophage Cells of White Mulberry (Morus alba L.) Leaf Extracts
Authors: Suriyaprom Sureeporn , Srisai Pitchayuth , Intachaisri Varachaya , Kaewkod Thida , Pekkoh Jeeraporn , Desvaux Mickaël , Tragoolpua Yingmanee
Published: May 2023

The white mulberry (Morus alba L.) is widely used as a medicinal plant in Asia. In this study, the bioactive compounds of ethanolic extracts of white mulberry leaves from the Sakon Nakhon and Buriram cultivars were evaluated. The ethanolic extracts of mulberry leaves from the Sakon Nakhon cultivar showed the highest total phenolic content of 49.68 mg GAE/g extract and antioxidant activities of 4.38 mg GAE/g extract, 4.53 mg TEAC/g extract, and 92.78 mg FeSO4/g extract using 2,2 diphenyl-1-picrylhydrazyl (DPPH), 2,20-azinobis-(3-ethylbenzothiazolin-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays, respectively. The resveratrol and oxyresveratrol compounds in mulberry leaves were also investigated by high-performance liquid chromatography (HPLC). The mulberry leaf extracts from the Sakon Nakhon and Buriram cultivars showed oxyresveratrol contents of 1.20 ± 0.04 mg/g extract and 0.39 ± 0.02 mg/g extract, respectively, whereas resveratrol was not detected. It was also found that the potent anti-inflammatory properties of mulberry leaf extracts and its compounds, resveratrol and oxyresveratrol, suppressed the LPS-stimulated inflammatory responses in RAW 264.7 macrophage cells by significantly reducing nitric oxide production in a concentration-dependent manner. These compounds further inhibited interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) production and suppressed the mRNA and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW 264.7 macrophage cells. Therefore, it is established that mulberry leaf extract and its bioactive compounds contribute to its anti-inflammatory activity.
Bioactive Phytochemicals of Acacia saligna
Authors: Ung Alison T. , Asmara Anjar P.
Published: May 2023

Acacia saligna is native to Western Australia. It has become an introduced and fast-growing plant in other parts of the world due to its ability to adapt to drought, saline and alkaline soils, and hast growing environments. Studies on the bioactivities and phytochemicals of the plant extracts were conducted. However, comprehensive information that links those bioactivities to the identified compounds in the plant’s extracts is still lacking. Data gathered in this review revealed a rich chemical diversity of hydroxybenzoic acids, cinnamic acids, flavonoids, saponins, and pinitols in A. saligna growing in Egypt, Saudi Arabia, Tunisia, South Africa, and Australia. The variability in phytochemical composition and quantity could be attributed to plant parts, growing locations, extraction solvents, and analysis methods. Identified phytochemicals support observed biological activities such as antioxidant, antimicrobial, anticancer, α-glucosidase inhibition, and anti-inflammation in the extracts. The knowledge of chemical structures, biological activities, and possible mechanisms of action of the bioactive phytochemicals identified in A. saligna were discussed. In addition, the structure–activity relationships of dominant active compounds were examined to explain the bioactivities exerted by A. saligna extracts. The review provides valuable insights towards future research and the development of new therapeutics from this plant.
Light-Elicited and Oxygen-Saved Iridium Nanocapsule for Oxidative Damage Intensified Oncotherapy
Authors: Chen Guobo , Wang Xiang , He Zongyan , Li Xueyu , Yang Zhijin , Zhang Yule , Li Yuhao , Zheng Lulu , Miao Yuqing , Zhang Dawei
Published: May 2023

Regulating redox homeostasis in tumor cells and exploiting oxidative stress to damage tumors is an efficacious strategy for cancer therapy. However, the strengths of organic nanomaterials within this strategy are often ignored. In this work, a light-triggered reactive oxygen species (ROS) damaging nanoamplifier (IrP-T) was developed for enhanced photodynamic therapy (PDT). The IrP-T was fabricated with an amphiphilic iridium complex and a MTH1 inhibitor (TH287). Under green light stimulation, IrP-T catalyzed the oxygen in cells to generate ROS for realizing oxidative damage; meanwhile, TH287 increased the accumulation of 8-oxo-dGTP, further strengthening oxidative stress and inducing cell death. IrP-T could maximize the use of a small amount of oxygen, thus further boosting the efficacy of PDT in hypoxic tumors. The construction of nanocapsules provided a valuable therapeutic strategy for oxidative damage and synergizing PDT.