bluuebunny/crossref_metadata_2025 huggingface dataset:
Poly-Temporal, Multi-Layered: A Techno-Cognitive Theory of Narrative Experience in Literature
Authors: Kurzynski Maciej
Published: February 2025

This article draws upon recent developments in cognitive neuroscience and natural language processing to contribute a techno-cognitive perspective into the ‘deep reading’ versus ‘surface reading’ debate in literary studies. Research at the intersection of humanities and sciences suggests that narrative experience, including both production (decoding) and reception (encoding) of stories, constitutes a sequentially and hierarchically complex process shaped simultaneously by socio-cultural contexts, sensory-emotional dynamics, and cognitive integration across multiple levels of complexity. This interdisciplinary view contrasts with traditional humanities methodologies such as area studies, which privileges identity-based accounts of literary phenomena, or Marxist genealogy, which neglects extra-political sources of meaning. The article surveys relevant research findings across multiple domains and discusses the hermeneutic implications of the techno-cognitive approach for literary studies, exemplified in a reading of Zhang Xianliang's 1985 novel Half of Man is Woman.
Impact of Genre on Authorship Attribution in Arabic Poetry and Prose
Authors: Mohamed Emad , Elewa Abdelhamid
Published: February 2025

This article examines whether authors change their styles when writing in different genres, like poetry and prose, particularly under the metrical constraints of the former. Using a corpus of works by five modern Egyptian authors, we analyse the effectiveness of N most frequent words and N most frequent morphological segments in distinguishing between poetry and prose written by the same author. Through supervised and unsupervised machine learning techniques, we demonstrate that authors exhibit distinct stylistic fingerprints in each genre, influenced by the unique conventions and constraints of each form. Results show that each author uses two different stylistic prints when writing prose and poetry. However, when mixing poetry and prose together, all genre-related texts cluster separately, potentially causing author obfuscation. Findings also show that the frequent word method is sufficient for accurately attributing authorship when it comes to mixed-genre texts. In short, the tested linguistic standard features prove resilient across different genres and even survive the constraints of formal poetic meter.
PENGEMBANGAN ALAT EVALUASI PEMBELAJARAN DENGAN APLIKASI GIMKIT PADA MATA PELAJARAN IPAS SISWA MADRASAH IBTIDAIYAH
Authors: Zumrotus Sa'diyah  , Rofi`'ah Firda Zakiyatur , Nurhayati Indah
Published: February 2025

Penggunaan alat evaluasi berbasis online pada saat ini merupakan bagian penting dalam penyelenggaraan pendidikan. Upaya peningkatan mutu pendidikan dapat dicapai melalui peningkatan mutu pembelajaran dan mutu penilaian. Kualitas pembelajaran dapat dilihat dari hasil evaluasi. Maka dari itu, diperlukannya pemanfaatan alat evaluasi berbasis online yang sudah ada, sehingga dapat dikembangkan dalam evaluasi pembelajaran IPAS. Tujuan penelitian ini yaitu Mengetahui bagaimana proses pengembangan alat evaluasi pembelajaran dengan aplikasi Gimkit pada mata pelajaran IPAS di kelas IV MI Tarbiyatul Islam Sokosari, dan Mengetahui hasil pengembangan alat evaluasi dengan aplikasi Gimkit pada mata pelajaran IPAS di kelas IV MI Tarbiyatul Islam Sokosari. Jenis penelitian ini yaitu research and development (R&D) dengan model 4D terdiri dari empat tahap utama, yaitu Define (Pendefinisian), Design (Perancangan), Develop (Pengembangan), Disseminate (Penyebaran). Hasil pengembangan alat evaluasi menggunakan aplikasi Gimkit pada mata pelajaran IPAS Kelas IV berupa alat evaluasi dengan jenis soal literasi membaca dengan jumlah 20 soal pilihan ganda, dan alat evaluasi dinyatakan layak digunakan dibuktikan dengan hasil validasi oleh ahli tampilan dengan persentase 94% kategori sangat layak, ahli soal dengan dengan persentase 92% kategori sangat layak serta respon dari siswa dengan persentase 93% kategori sangat layak.
PENGARUH PEMBELAJARAN KOOPERATIF TIPE STAD (STUDENT TEAM ACHIEVEMENT DIVISION) TERHADAP HASIL BELAJAR LITERASI NUMERASI SISWA
Authors: Khoiroh Siti Zumarotul , Andreastya Vian Hanes
Published: February 2025

Pembelajaran Kooperatif STAD merupakan pembelajaran secara  berkelompok bertujuan untuk meningkatkan prestasi belajar peserta didik, dengan pembelajaran  matematika, dengan literasi numerasi program K.13 dalam kurikulum merdeka. Yang bertujuan untuk mendeskripsikan pengaruh Pembelajaran Kooperatif STAD Terhadap Hasil Belajar Literasi Numerasi, di Madrasah Ibtidaiyah Islamiyah Ngoro. Peneliti ini menggunakan pendekatan kuantitatif dengan desain true eskperimental design dengan tipe posttest-only control design. Dengan teknik purposive sampling. Pengumpulan data dilakukan dengan menggunakan teknik interview, dokumentasi, observasi, dan instrumen sebagai data penelitian. Analisis yang dilakukan oleh peneliti dengan; uji validitas, reabilitas, uji hipotesis dan uji mann whetney. Dari hasil uji analisis reabilitas diketahui conbach’s alpha 0,864  >0,06 dari uji validitas. Dinyatakan bahwa butir tes hasil belajar literasi numerasi terhadap metode pembelajaran STAD telah reliabilitas. Sedangkan dalam uji statistik menggunakan uji Man Whitney dengan singnifikan diperoleh dengan nilai asymp.sig.(2-tailed) sebesar 0,002 < 0,05 dari hasil posttest-only control desain kelas eksperimen dan kelas kontrol,  skor yang dihasilkan dari kelas eksperimen 1432 sedangkan kelas kontrol 880 dengan nilai rata-rata 32. Maka dari itu bisa disimpulkan bahwa Ha diterima H0 ditolak yang artinya, ada pengaruh pembelajaran kooperatif (STAD) Student Team Achievement Division terhadap literasi numerasi di MI Islamiyah Ngoro Jombang.
PENGARUH MEDIA MATH BINGO TERHADAP HASIL BELAJAR MATERI RASIO KELAS V SDN LARANGAN SORJAN 2
Authors: Mauliyana  , Zahrani Susilo Claudya
Published: February 2025

The use of innovative learning media is essenstial in helping students comprehend abstract mathematical concepts, including ratio. One of the media that can be utilized is Math Bingo, whish is designed to create an interactive and engaging learning environtment. This  study aims to describe the influence of Math Bingo on the learning outcomes of fifth  grade students at SDN Larangan Sorjan 2 Klampis Bangkalan in ratio material. The research method employed is a quasi experimental design with a pretest postest control group, involving a sample of 27 fifth grade students at SDN Larangan Sorjan 2 Klampus Bangkalan. Data were collected through observation, interviews, and pretest-postetst techniques. Analysis using t-test yielded a t-value of 1.705619, and with t-table value at df 26 and a significance level of 0,05 being 0,00000030, t-calculated >t-table (1,705619>0,00000030). Additionally, the significance level was less than 0,05 (0,00000030 < 0,05). Thus, according to the t-test criteria , H0 is rejected, and H1 is accepted, indicating a significant influence on the learning outcome of fofth grade students at SDN Larangan Sorjan 2 Klampis Bangkalan
TANTANGAN DAN STRATEGI PENGINTEGRASIAN ILMU RUMPUN IPS DALAM PEMBELAJARAN DI PGMI : MEMBANGUN KOMPETENSI GURU UNTUK PENDIDIKAN BERKUALITAS
Authors: Defirra Alizunna  , Fatikh Inayahtur Rahma
Published: February 2025

This article discusses the challenges and strategies of integrating Social Studies in Madrasah Ibtidaiyah (PGMI) Teacher Education learning with a focus on discussing the complexity of social studies materials, differences in student learning styles, and learning accessibility gaps. Analysis of the literature reveals that teachers face difficulties in bridging the division between social studies content and Islamic values, requiring specific strategies to create an integrated learning experience. In this context, the main strategies proposed involve the application of technology in teaching to link social studies materials with Islamic values, curriculum development that is responsive to student diversity, and learning differentiation strategies that suit individual needs. This article aims to provide practical guidance for teachers, researchers and policy makers in facing the challenges of integrating social studies in the school environment, building teacher competencies and creating classrooms that integrate diversity as a valuable resource. The conclusion of this article is expected to make a significant contribution to the development of future strategies in achieving quality social studies education.
IMPLEMENTASI ACTIVE LEARNING BERBASIS VIDEO ANIMASI INFOGRAFIS PADA PELAJARAN BAHASA INDONESIA DI MI AL URWATUL WUTSQO 1
Authors: Laily Hikmatul , Mahsun Ali
Published: February 2025

Indonesia language learning at the madrasah ibtidaiyah level requires innoative methods and media to make it easier for students to understand the material and develop their language skills. On of the methods and media that can be used is active learning based on animated infograpic videos. This research aims to analyze the implementation of active learning using animared infographic videos in Indonesian language learning at MI Al Urwatul Wutsqo 1. The research method is descriptive qualitative with 6th grade students as the research subjects. The animated infograohic video based learning media is applied to improve students understanding of the subject matter. The research result show that the use of animated infographic videos in the active learning model can enchance students learning outcomes and increase their undestanding of indonesia languasge learning. It also provides motivation, enthusiasm, and fosters discussion. Additionally, students show a higher level of interest and oarticipation in Indonesia language learning. Based on these results, it can concluded that this media is recommended for use in Indonesian langauge learning at schools.
The Security Council in Its Entirety: Unveiling 80 Years of Deliberation through the UNSC Meetings and Speeches Dataset
Authors: Sakamoto Takuto , Matsuoka Tomoyuki , Ito Hiroto
Published: February 2025

This article presents a new dataset that compiles every available public transcript of the United Nations Security Council (UNSC) from 1946 to 2024 in a machine-readable format. Encompassing over one hundred thousand speeches and more than eighty-seven million words, it offers unprecedented historical depth, capturing both Cold War–era debates and post–1990 expansions in Council activities. The dataset enables analyses of meeting frequencies, resolution adoption rates, evolving rhetorical emphases (e.g., on “sovereignty” versus “human rights”), thematic patterns revealed by topic modeling, and shifts in sentiment across different periods. By spanning nearly eight decades of deliberations, this resource supports diverse research agendas on global security norms, the roles of major powers and smaller states, and the expanding influence of UN officials in shaping Council discourse.
The Influence of Operating Cash Flow, Current Ratio, and Solvability Ratio on Profit Margin in Service Companies
Authors: Novyarni Nelli , Tika Amalia Bahagia  , Rutinaias Haholongan  , Reni Harni  , Kartijo Kartijo
Published: February 2025

This research aims to determine the influence of operating cash flow, current ratio and solvency ratio on profit margin in service companies in the financial institution sub-sector listed on the Indonesia Stock Exchange in 2019-2022. This research uses a type of quantitative research which is measured using a regression-based method, namely the t test and f test using the Eviews version 12 program. The data used in this research is secondary data. The data obtained is pool data. Data collection was obtained from 9 companies consisting of 36 reports finance. The sampling method uses purposive sampling technique. Data source obtained from www.idx.co.id.
The research results prove that (1) operating cash flow has an effect on profit margin, (2) the current ratio has no effect on profit margin, (3) the solvability ratio has no effect on profit margin, (4) the influence of the current ratio and the solvency ratio simultaneously has an effect on profit margin.
Série e linguagem multimodal: estratégias para o desenvolvimento da leitura crítica e das competências 1 e 2 da BNCC
Authors: Ildebrand Isaías dos Santos
Published: February 2025

O Este estudo investiga o potencial didático da série Severance (Erickson, 2022) no ensino de Língua Portuguesa para o 3º ano do Ensino Médio, com foco no desenvolvimento das Competências 1 e 2 da BNCC (Brasil, 2018). Considerando as transformações tecnológicas e culturais, a pesquisa parte do pressuposto de que narrativas audiovisuais, enquanto textos multimodais, podem contribuir significativamente para a leitura crítica e argumentativa dos estudantes. O objetivo é analisar como os temas abordados na série, como fragmentação da identidade, controle institucional e alienação do trabalho, podem ser utilizados pedagogicamente para fomentar discussões discursivas e sociais em sala de aula. A metodologia adotada consiste em uma abordagem qualitativa, ancorada nos conceitos de letramento crítico e multimodalidade (Rojo, 2012; Kress & Leeuwen, 2002; Ildebrand, 2021). O estudo explora a relação entre linguagem, discurso e tecnologia, demonstrando como a narrativa audiovisual pode ser integrada ao ensino para ampliar as possibilidades interpretativas dos alunos. Os resultados indicam que o uso de Severance promove um ensino mais dinâmico, estimulando a construção de sentidos e a argumentação crítica. Conclui-se que a adoção de narrativas seriadas no ensino de línguas fortalece o protagonismo estudantil, tornando a aprendizagem mais significativa e alinhada às demandas contemporâneas, além de contribuir para o desenvolvimento da autonomia intelectual e da consciência discursiva dos estudantes.
Defining optimal electrospun membranes to enhance biological activities of human endometrial MSCs
Authors: An Jiangru , Ma Tianyi , Wang Qiuhua , Zhang Jinyi , Santerre J. Paul , Wang Wenshuang , Ma Peng , Zhang Xiaoqing
Published: February 2025

Introduction

Human endometrial mesenchymal stem cells (H-EMSCs) can inhibit endometrial fibrosis and repair damaged endometrium. However, direct cell injection into dam-aged endometrium shows limited cell survival. Cell seeding onto biomaterial-based electrospun membranes could improve H-EMSCs’ survival and prolong their stay at the damaged endometrium. Polycaprolactone (PCL), silk fibroin (SF) and hyaluronic acid (HA) are synthetic or natural biomaterials used by the biomedicine field, however, their effects on the biological activities of H-EMSCs remain unclear.

Methods

In this study, CD90+CD73+CD45− H-EMSCs were extracted from human endometrium and H-EMSCs showed enhanced adhesion, proliferation on PCL-HA vs. PCL, PCL-SF, establishing the potential of the composite to address cell survival issues.

Results

H-EMSCs cultured on PCL-HA showed decreased IL-6 gene expression and increased IL-10, VEGFA, TGF-β gene expression vs. PCL-SF, establishing the potential to create a favorable micro-environment for generating vascularized endometrial tissues. PCL, PCL-SF, PCL-HA all supported CD90 and Meflin expression of the seeded H-EMSCs, establishing PCL as a platform to form enhanced biomaterial composites for endometrial repair in the future.

Discussion

This study provided significant evidence sup-porting the potential of appropriately tailored composites of PCL and HA to moder-ate inflammation and wound-healing, which can be applied for endometrial tissue repair and regeneration.
Gut microbiota therapy in gastrointestinal diseases
Authors: Ullah Hanif , Arbab Safia , Chang Chengting , Bibi Saira , Muhammad Nehaz , Rehman Sajid Ur , Suleman  , Ullah Irfan , Hassan Inam Ul , Tian Yali , Li Ka
Published: February 2025

The human gut microbiota, consisting of trillions of microorganisms, plays a crucial role in gastrointestinal (GI) health and disease. Dysbiosis, an imbalance in microbial composition, has been linked to a range of GI disorders, including inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), celiac disease, and colorectal cancer. These conditions are influenced by the interactions between the gut microbiota, the host immune system, and the gut-brain axis. Recent research has highlighted the potential for microbiome-based therapeutic strategies, such as probiotics, prebiotics, fecal microbiota transplantation (FMT), and dietary modifications, to restore microbial balance and alleviate disease symptoms. This review examines the role of gut microbiota in the pathogenesis of common gastrointestinal diseases and explores emerging therapeutic approaches aimed at modulating the microbiome. We discuss the scientific foundations of these interventions, their clinical effectiveness, and the challenges in their implementation. The review underscores the therapeutic potential of microbiome-targeted treatments as a novel approach to managing GI disorders, offering personalized and alternative options to conventional therapies. As research in this field continues to evolve, microbiome-based interventions hold promise for improving the treatment and prevention of gastrointestinal diseases.
Mobile public warning in Japan and the United States: a sister cities collaboration
Authors: Bean Hamilton , Takenouchi Kensuke , Cruz Ana Maria
Published: February 2025

Over the last decade, countries have adopted or expanded the use of cell broadcast systems to support mobile public warning for natural and human-caused hazards and disasters. Cell broadcast entails sending short messages simultaneously to multiple mobile devices in a defined geographic area. Japan and the United States have pioneered the development of mobile public warning technology, yet both countries continue to experience problems with the nonuse, misuse, or misunderstanding of these systems. To explore prospects for improving this situation, this study presents thematic analyses of official documents and transcripts from four Japan-U.S. meetings and workshops with researchers, officials, and residents in the “sister cities” of Yamagata, Yamagata Prefecture, Japan, and Boulder, Colorado, USA. The study’s boundary spanning, cross-national findings contribute new insights regarding the appropriate level of local adaptation versus global standardization of mobile public warning theory, policy, and practice. These findings can aid officials who are increasingly relying on mobile public warning systems to help keep communities safe amid the intensifying impacts of climate change.
Burden, risk factors, and projections of ischemic heart disease in China (1990–2021): findings from the 2021 GBD study
Authors: Xu Sikai , Liu Zhiyang , Tang Mu , Xu Chunli
Published: February 2025

Background

Ischemic heart disease (IHD) remains a major public health challenge in China. This study aimed to comprehensively analyze the burden of IHD, its risk factors, and future trends from 1990 to 2021 using the Global Burden of Disease database.

Methods

We assessed IHD trends in incidence, prevalence, mortality, and disability-adjusted life years (DALYs) stratified by age (greater than 15 years) and gender. Age-standardized rates, average annual percentage changes, and joinpoint regression analyses were used to evaluate temporal trends. Decomposition and frontier analyses were conducted to identify key contributors to the IHD burden, while future projections were generated for the next 15 years.

Results

In 2021, the number of IHD incident cases, prevalent cases, deaths, and DALYs in China were 3.17, 3.25, 3.57, and 2.62 times higher than those in 1990, respectively. Age-standardized mortality rates and age-standardized DALYs rates demonstrated an initial increase, followed by a gradual decline. Males showed higher IHD burden during middle age, while elderly females surpassed males in the later years. Aging, high systolic blood pressure, ambient particulate matter pollution, elevated low-density lipoprotein cholesterol, and smoking were the primary drivers of IHD burden. Future projections suggest a declining incidence and prevalence among males but increasing trends in females, with DALYs expected to rise significantly in the female population.

Conclusions

The burden of IHD in China has evolved significantly over the past three decades, driven by demographic and environmental factors. While prevalence and incidence have risen, mortality and DALYs have shown a recent decline, reflecting shifts in disease patterns. Age and gender disparities are evident, with middle-aged males and elderly females disproportionately affected. Key contributors, such as high blood pressure and pollution, highlight the need for targeted interventions. Gender-specific public health strategies, alongside improved environmental and health policies, are essential to mitigating the future burden of IHD in China.
Prediction of the change in suitable growth area of Sabina tibetica on the Qinghai-Tibetan plateau using MaxEnt model
Authors: Li Xiaoxiong , Yang Dongsheng , Wang Jingjie , Pan Gang
Published: February 2025

As a keystone species maintaining alpine ecosystem stability, Tibetan juniper (Sabina tibetica) is endemic to the Qinghai-Tibetan Plateau, thriving at 2,800–4,600 m elevations. We employed the MaxEnt model with 10 bioclimatic and topographic variables to predict its distribution shifts under RCP4.5 and RCP8.5 scenarios for 2050 and 2070. Model performance was validated through five-fold spatial cross-validation (AUC = 0.932), utilizing 99 occurrence records from field surveys and biodiversity databases. Minimum winter temperature (35.1% contribution) and warmest quarter precipitation (18.9%) emerged as dominant drivers. The current suitable habitat (4.69 × 104 km2) is projected to decrease to 3.82 × 104 km2 (18.6% reduction) under RCP4.5-2050 and 2.78 × 104 km2 (40.7% reduction) by 2070. Under high-emission scenarios, suitable areas will contract to 3.83×10⁴ km² (RCP8.5-2050) and 3.86 × 104 km2 (RCP8.5-2070), showing 18.3% and 17.7% reductions respectively. Range contractions concentrate in western Sichuan and southeastern Tibet, with RCP4.5-2070 exhibiting the most severe habitat loss. with range contractions concentrated in western Sichuan and southeastern Tibet. Priority conservation areas were identified in the Yarlung Zangbo Valley and Hengduan Mountains. This study provides quantitative assessment of Sabina tibetica’s climate vulnerability, offering critical insights for adaptive management of high-altitude ecosystems under global change.
Shoreline_veget— a new shoreline extraction method for vegetation seas, with high robustness, accuracy and scalability
Authors: Li Weihua , Liu Lianglin , Zhu Wei , Li Jiuming , Liu Teng
Published: February 2025

To address the issue of vegetation obstructing water bodies and resulting in missing information in vegetation sea areas, existing methods that focus on various types of shorelines often exhibit limited algorithm stability and accuracy. This study introduces a method, termed Shoreline_veget. The method comprises four modules: data preprocessing, point cloud boundary extraction and processing, elevation gradient function design, modified fused boundary point cloud, and tidal correction. This method can reduce the overall shoreline accuracy from 0.6658, 0.3854, and 0.4127 (as observed with three comparative methods) to 0.1531. Compared to the least accurate method, this method improved the overall shoreline accuracy by 0.5127 m. The result confirm that the proposed method offers superior stability, and this methodology provides new technology to measure, map, and manage shorelines, offers valuable insight for related research.
Mesoporous carbon materials: synthesis methods, properties, and advanced applications
Authors: Wei Zhou , Yanfei Zhi , Jiao Wang
Published: February 2025

Mesoporous carbon materials have great potential in energy storage, catalysis and adsorption separation due to their unique pore size distribution, high specific surface area and excellent stability. This review discusses the synthesis methods of mesoporous carbon materials. The comparative analysis emphasizes the advantages and limitations of different preparation methods, providing a basis for the targeted design of mesoporous carbon materials. The properties of mesoporous carbon (high specific surface area, electrochemical, thermal stability, etc.) are introduced in detail, and the relationship between the physicochemical properties of mesoporous carbon and its electrochemical and adsorption properties is discussed, and the contribution of structural regulation to performance improvement is clarified. In addition, the practical applications of mesoporous carbon materials in supercapacitors, lithium-ion batteries, adsorption and catalysis are discussed. Challenges such as stability, cost-effectiveness and scalability are pointed out, and future research prospects in functional modification, precise structural design and environmentally sustainable synthesis are envisioned.
Impact of physical activity on all-cause mortality in individuals with non-cystic fibrosis bronchiectasis
Authors: Kim Sang Hyuk , Choi Hayoung , Han Kyungdo , Jung Jin-Hyung , Yang Bumhee , Lee Hyun
Published: February 2025

Background

Little information is available regarding whether active physical activity lowers mortality risk in individuals with bronchiectasis.

Methods

We used the Korean National Health Insurance Service database from 2010 to 2016 to evaluate the association between changes in physical activity and mortality risk in individuals with bronchiectasis. Of 552,510 individuals with newly diagnosed bronchiectasis, we enrolled 165,842 individuals who had two consecutive health examinations before and after bronchiectasis diagnosis, within two years, as the study aimed to measure changes in exercise habits between the two time points. Active physical activity was defined as engaging in moderate- or vigorous-intensity physical activity at least once a week, either before or after bronchiectasis diagnosis. The outcome measure was all-cause mortality.

Results

During a mean follow-up of 6.2 ± 2.1 years, 10,535 (6.4%) individuals with bronchiectasis died. Individuals with bronchiectasis who were physically active exhibited a lower mortality rate than those who were physically inactive. Mortality reduction was particularly evident in the exercise maintainers group (aHR [adjusted hazard ratio] = 0.69, 95% confidence interval [CI] = 0.64–0.74) and individuals with physical activity ≥1,000 metabolic equivalent of task-min per week (aHR = 0.73, 95% CI = 0.70–0.77) compared to those who were physically inactive.

Conclusion

Engaging in active physical activity is associated with a decreased risk of mortality in individuals with bronchiectasis.
Advancing chronic and acute wound healing with cold atmospheric plasma: cellular and molecular mechanisms, benefits, risks, and future directions
Authors: Raissi-Dehkordi Nastaran , Raissi-Dehkordi Negar , Ebrahimibagha Hamed , Tayebi Tahereh , Moeinabadi-Bidgoli Kasra , Hassani Mohammad , Niknejad Hassan
Published: February 2025

Chronic and acute wounds represent significant challenges in healthcare, often leading to prolonged recovery times and increased complications. While chronic wounds, such as diabetic foot ulcers and venous leg ulcers, persist due to underlying conditions and biofilm formation, acute wounds, including surgical incisions and burns, can also benefit from innovative therapeutic approaches. Cold atmospheric plasma (CAP) has emerged as a promising non-invasive therapy capable of enhancing wound healing outcomes across both wound types. This review examines the cellular and molecular mechanisms by which CAP promotes wound repair, focusing on its modulation of inflammation, stimulation of angiogenesis, facilitation of tissue remodeling, and antimicrobial effects, which can potentially be used in regenerative medicine. CAP generates reactive oxygen and nitrogen species that influence key cellular processes, accelerating tissue regeneration while reducing bacterial load and preventing biofilm formation. Clinical applications of CAP have demonstrated its efficacy in improving wound healing metrics for both chronic and acute wounds. Despite promising results, translating CAP into routine clinical practice requires addressing challenges such as standardizing treatment protocols, assessing long-term safety, and developing portable devices. Future research should prioritize optimizing CAP parameters and exploring combination therapies to maximize its therapeutic potential. Overall, CAP represents a safe, effective, and versatile modality in wound management, with the potential to significantly improve patient outcomes in both chronic and acute wound care.
Prognostic value of c-MET in oesophageal squamous cell carcinoma: a study based on the mRNA expression in TCGA database and a meta-analysis
Authors: Zhang Qiqi , Li Xiujuan , Li Jian , Zhang Zhiqiang
Published: February 2025

Objective

This study aims to assess the mesenchymal-epithelial transition factor’s (c-MET) prognostic value in oesophageal carcinoma (ESCA) through a meta-analysis and bioinformatics.

Methods

We analysed c-MET expression in ESCA tissues using data from The Cancer Genome Atlas (TCGA) and conducted a meta-analysis to evaluate its association with clinicopathological factors and survival outcomes. The meta-analysis included studies reporting hazard ratios (HRs) and odds ratios (ORs) for survival and metastatic outcomes.

Results

The Cancer Genome Atlas analysis revealed elevated c-MET expression in ESCA, which was significantly correlated with lymph node metastasis, tumour grade and stage, though not with overall survival (OS). In the meta-analysis, 278 publications were identified, and 89 duplicates were removed. After screening, 176 articles were excluded, leaving 13 for full-text review. Of these, 5 studies lacked sufficient survival data, resulting in 8 eligible studies with a total of 1,488 patients. Meta-analysis findings indicated that high c-MET expression was associated with worse OS (HR = 1.54, 95% confidence interval [CI]: 1.17–2.01; p = 0.002), distant metastasis (OR = 1.97, 95% CI: 1.14–3.40; p = 0.02) and advanced stage (OR = 2.23, 95% CI: 1.41–3.53; p = 0.0006).

Conclusion

High c-MET expression is associated with poor prognosis and advanced disease in ESCA, highlighting its potential as a biomarker for risk stratification. Further studies are needed to confirm its prognostic value and explore therapeutic implications.
Plasmonic slot waveguides: a quantum leap in nonlinear nanophotonics
Authors: Rojas Yanez Libertad , Hu Huatian , Ciracì Cristian , Palomba Stefano
Published: February 2025

Interest and excitement in nanophotonics—the study and control of light-matter interactions at the nanoscale—are driven by the ability to confine light to volumes well below a cubic wavelength, and, thereby, achieve extremely high intensities. This leads to light-matter interactions of unprecedented localization and strength. Such extreme behavior—both in terms of field enhancement and localization—can be achieved using plasmonic nanostructures, which concentrate light in regions much smaller than the wavelength of light, reducing the excitation power and, under certain conditions, removing phase-matching requirements in the nonlinear regime. In this study, we theoretically show that metal–dielectric–metal (MDM) slot waveguides (WGs), consisting of a thin dielectric layer sandwiched between metal films, provide the strongest confinement. We also demonstrate that integrating epsilon-near-zero (ENZ) materials within the MDM slot significantly improves the nonlinear conversion efficiency of these structures. The results show that the degenerate four-wave mixing conversion efficiency of these ENZ-MDM structures surpasses that of regular plasmonic structures and their dielectric counterparts, even under low pump power conditions, and remains robust despite higher losses in the ENZ material.
Autism spectrum disorder related phenotypes in a mouse model lacking the neuronal actin binding protein profilin 2
Authors: Witke Walter , Di Domenico Marina , Maggi Laura , Di Nardo Alessia , Stein Valentin , Pilo Boyl Pietro
Published: February 2025

Introduction

Profilin 2 (PFN2) is an actin binding protein highly expressed in the brain that participates in actin dynamics. It has been shown in vitro and in vivo that in neurons it functions both post-synaptically to shape and maintain dendritic arborizations and spine density and plasticity, as well as pre-synaptically to regulate vesicle exocytosis. PFN2 was also found in protein complexes with proteins that have been implicated in or are causative of autism spectrum disorder.

Methods

We employ a genetically engineered knock-out mouse line for Pfn2 that we previously generated to study the mouse social, vocal and motor behavior in comparison to wild type control littermates. We also study neuronal physiology in the knock-out mouse model by means of cellular and field electrophysiological recordings in cerebellar Purkinje cells and in the Schaffer collaterals. Lastly, we study anatomical features of the cerebellum using immunofluorescence stainings.

Results

We show that PFN2 deficiency reproduces a number of autistic-like phenotypes in the mouse, such as social behavior impairment, stereotypic behavior, altered vocal communication, and deficits in motor performance and coordination. Our studies correlate the behavioral phenotypes with increased excitation/inhibition ratio in the brain, due to brain-wide hyperactivity of glutamatergic neurons and increased glutamate release not compensated by enhanced GABAergic neurotransmission. Consequently, lack of PFN2 caused seizures behavior and age-dependent loss of cerebellar Purkinje cells, comorbidities observed in a subset of autistic patients, which can be attributed to the effect of excessive glutamatergic neurotransmission.

Discussion

Our data directly link altered pre-synaptic actin dynamics to autism spectrum disorder in the mouse model and support the hypothesis that synaptic dysfunctions that asymmetrically increase the excitatory drive in neuronal circuits can lead to autistic-like phenotypes. Our findings inspire to consider novel potential pathways for therapeutic approaches in ASD.
GABAergic integration of transient and persistent neurons in the developing mouse somatosensory cortex
Authors: Abusaada Ahd , De Rosa Federico , Luhmann Heiko J. , Kilb Werner , Sinning Anne
Published: February 2025

GABA is an essential element in the function of neocortical circuits. The origin, migration and mechanisms of synaptogenesis of GABAergic neurons have been intensively studied. However, little information is available when GABAergic synapses are formed within the different cortical layers, neuronal cell types and subcellular compartments. To quantify the distribution of GABAergic synapses in the immature somatosensory mouse cortex, GABAergic synapses were identified by spatially coincident immunoprofiles for the pre- and postsynaptic markers vGAT and gephyrin at postnatal days (P)0-12. Between P0-5, GABAergic synapses are mainly restricted to the marginal zone, while at later developmental stages a more homogenous distribution is obtained. Cajal-Retzius neurons represent a major target of GABAergic synapses in the marginal zone with a homogeneous synapse distribution along the dendrite. The number of GABAergic synapses per pyramidal neuron increases substantially between P0 and P12, with a stable density and distribution in basal dendrites. In contrast, along apical dendrites synapses accumulate to more proximal positions after P8. Overall, the results of this study demonstrate that early GABAergic synaptogenesis is characterized by a consistent increase in the density of synapses with first a stringent overrepresentation in the marginal zone and a delayed establishment of perisomatic synapses in pyramidal neurons.
Construction and evaluation of a finger motor feedback system to improve finger dexterity
Authors: Takahashi Shingo , Sakurai Noriko , Kuroiwa Yuki , Takahashi Daishi , Kodama Naoki
Published: February 2025

Introduction

Recently, a link has been established between cognitive function and hand dexterity in older adults. Declines in cognitive function have been shown to impair performance in finger tapping movements. Research suggest that hand training can improve dexterity, executive function, and cognitive function over time. This underscores the need for effective methods to improve hand and finger dexterity.

Method

In this study, we introduced a new hand training system that provides real-time feedback on finger movements during tapping tasks. We examined the system's impact on the finger dexterity of 32 healthy young participants by using a magnetic sensor finger tapping device (UB-2). During the finger tapping task, the participants performed opening and closing movements either in-phase or anti-phase on both left and right hands for 15 s. They were instructed to tap as quickly as possible. The number of taps, left–right balance, and other relevant data were measured using the UB-2 device.

Results

In terms of the number of tapping, a significant difference was found between 64.4 without feedback and 68.1 with feedback for the simultaneous opening and closing movements in the dominant hand. In the alternating open-close movement, the significant difference was 50.3 without feedback and 53.4 with feedback. The results showed that the system significantly improved the number and frequency of taps for both hands.

Conclusion

The improved tapping performance with feedback suggests that this system can improve hand dexterity.
A multicenter mixed-methods study on the effects of intermittent fasting in patients with immune thrombocytopenia receiving thrombopoietin receptor agonists
Authors: Yassin Mohamed A. , AlRasheed Muna , Al-Eisa Taghreed , Alhashim Noura , Alsayegh Fiasal , Abouzeid Tarek E. , Abd El Fattah Mohamed , Alfili Maryam , Shalaby Neveen , Alotaibi Abdullah , Aljuwaisri Nourah , Almasbahi Anwar , Saleeb Rii , Abdelaziz Hend , Alshurafa Awni , Ismail Omar , Ghasoub Rola
Published: February 2025

Introduction

In recent years, significant advances have been made in the treatment of immune thrombocytopenia (ITP) with the development of thrombopoietin receptor agonists (TPO-RAs). TPO-RAs are often used following the failure of prior therapies or when bleeding episodes persist despite glucocorticoid use. In Muslim countries, where religious observance includes 16/8 intermittent fasting, the timing of medication administration may be affected. This study is the first to evaluate the impact of Ramadan fasting on patients receiving different TPO-RAs.

Methods

A multicenter mixed-design study was performed in which Muslim patients who fasted during Ramadan while receiving TPO-RAs were interviewed between 2015 and 2023. Patient responses before, during, and after Ramadan were evaluated retrospectively. The bleeding tendency was assessed as (1) no bleeding, (2) minor cutaneous/mucosal bleeding, or (3) severe bleeding that involves major organs.

Results

The present study included 100 patients from three Muslim countries, including Qatar, Kuwait, and Saudi Arabia, across four tertiary centers. A complete response was observed in 63% of patients on ROM, 46% on ELT and 37% on AVA. For AVA, the mean platelet (PLT) count before Ramadan was estimated at [146.11 ± 111.76], while during Ramadan, it dropped to [131.7 ± 107.6]. For patients on ELT, the mean PLT count before Ramadan was estimated at [120.02 ± 59.7], while during Ramadan, it dropped to [100.8 ± 68.16] (p = 0.016). For patients on ROM, the mean platelet count before Ramadan was estimated at [122.68 ± 80.57], while during Ramadan, it was [130.94 ± 84.96]. Only 3% (3 patients on ELT) experienced bleeding episodes.

Conclusion

This study supports the feasibility of Ramadan fasting for ITP patients receiving TPO-RAs. Further studies with a larger sample size are recommended to investigate the impact of other types of fasting on the efficacy and safety of TPO-RAs.
Effects of propolis intake on endurance exercise and molecular signaling related to inflammation and oxidative stress
Authors: Xu Xiaoying , Hu Bing , Qu Xiaorong
Published: February 2025

Honey bees extract sticky material from the exudates of different plants which transform afterwards to propolis. Propolis from several global locations has been shown to contain a wide variety of polyphenolic chemicals. Recent studies have revealed that propolis possesses antioxidant, anti-inflammatory, and immunomodulatory abilities. In laboratory animal studies, it has been demonstrated that propolis can enhance the functioning of the antioxidant defense system and decrease the activity of nuclear factor-kappa B. As a result, they can effectively alleviate the damage caused by exercise. One of the main flavonoids found in propolis, quercetin, has been demonstrated to enhance muscle mitochondrial biogenesis and exercise capacity. Propolis may aid athletes in preventing oxidative and inflammatory damage to their muscles during exercise and enhance their athletic performance. The goal of the current review was to evaluate how propolis consumption affected the molecular signaling associated with antioxidant/oxidant state, pro/anti-inflammatory cytokines, and anaerobic/aerobic endurance.
Association of pyridoxal 5′-phosphate (PLP) with lipid profiles: a population-based cohort study
Authors: Zhang Ru-yang , Chen Yue , Yan Xin-Qi , Zhang Yue , Zhou Hua , Feng Qian
Published: February 2025

Objectives

This study aims to explore the correlation between Pyridoxal 5′-Phosphate (PLP) levels and lipid profiles in adult individuals, utilizing data from the National Health and Nutrition Examination Survey (NHANES) database.

Methods

The research included individuals aged 20 years and above, extracted from the NHANES database, covering the period from 2005 to 2010. The primary objective was to scrutinize the relationship between PLP and lipid profiles. This was accomplished by employing weighted, multivariable logistic regression to ascertain these associations. Furthermore, to assess the variability within different demographic segments, interaction analyses were conducted. Additionally, restricted cubic spline (RCS) methodology was implemented to delve into potential nonlinear dynamics between PLP concentrations and lipid levels.

Results

A cohort of 6,459 individuals was included in this study. Our data indicated that 51.60% of the participants were under 50 years old, while 48.40% were over 50, comprising 48.83% males and 51.17% females. PLP levels demonstrated a negative correlation with low-density lipoprotein cholesterol (LDL-C) levels. After controlling for confounding variables, a one-unit increment in PLP correlates with a reduction of 17.7% in LDL-C concentrations (OR: 0.823, 95% CI: 0.823–0.824, p < 0.001). PLP levels exhibited a positive correlation with high-density lipoprotein cholesterol (HDL-C), which increased as PLP levels rose. After controlling for all covariates, a one-unit increase in PLP levels corresponded to a 1.952-fold enhancement in the probability of high HDL-C levels (OR: 1.952, 95% CI: 1.951–1.953, p < 0.001). The relationship between PLP and HDL-C levels was nonlinear. Subgroup analyses indicated that PLP levels and HDL-C concentrations are positively correlated, especially among diabetic patients and non-drinkers.

Conclusion

PLP levels are inversely associated with LDL-C and positively associated with HDL-C, with stronger effects observed in diabetic patients and non-drinkers. These findings underscore the potential clinical utility of PLP supplementation as a preventive measure against cardiovascular and metabolic diseases.
Sacroplasty with or without screw fixation for sacral metastatic tumors
Authors: Cox Evan P. , Tutton Sean , Scheidt Matthew , Key Brandon M. , Neilson John C. , Wooldridge Adam N. , Bedi Meena , Hackbarth Donald A. , King David M.
Published: February 2025

Introduction

Cementation (sacroplasty) with or without ablation has been shown to improve pain and function for patients with sacral metastatic disease. Percutaneous screw fixation with sacroplasty (PSFS) may provide superior outcomes in select patients.

Methods

Thirty patients with sacral metastases who underwent sacroplasty with or without ablation and screw fixation at a single institution were retrospectively reviewed. Patients were compared based on treatment (PSFS or sacroplasty alone) and fracture status (pathological or impending) with an ANCOVA. Traumatic fractures were excluded. Patients were followed for 4.4 months on average (range, 2 weeks to 36.5 months). Functional outcomes were assessed using the Musculoskeletal Tumor Society (MSTS) score. The rate of secondary procedures as well as changes in narcotic usage were noted.

Results

Patients with pathological fractures who underwent PSFS demonstrated increased postoperative MSTS scores compared to those who underwent sacroplasty (51% ± 19 versus 25% ± 13, p = 0.005). Patients with impending pathological fractures who underwent PSFS did not demonstrate statistically significant increased postoperative MSTS scores compared to those who underwent sacroplasty alone (38% ± 17 versus 32% ± 12, p = 0.72).

Discussion

PSFS may provide additional benefit for patients with pathological fractures, while sacroplasty alone may be sufficient for those with impending pathologic fractures secondary to sacral metastatic disease. This study was limited by its retrospective design and sample size; however, the results may aid in treatment indications for sacral metastases and guide further research Level of Evidence Level III, Therapeutic Study.
Multi-omics analysis identifies a liquid-liquid phase separation-related subtypes in head and neck squamous cell carcinoma
Authors: Zhai Peng-Lei , Chen Meng-Min , Wang Qi , Zhao Jing-Jun , Tang Xiao-Mei , Lu Cui-Ni , Liu Jia , Yang Qin-Xin , Xiang Ming-Liang , Tang Qing-Hai , Gu Biao , Zhang Shu-Ping , Tang Si-Ping , Fu Da
Published: February 2025

Background

Growing evidence indicates that abnormal liquid–liquid phase separation (LLPS) can disrupt biomolecular condensates, contributing to cancer development and progression. However, the influence of LLPS on the prognosis of head and neck squamous cell carcinoma (HNSCC) patients and its effects on the tumor immune microenvironment (TIME) are not yet fully understood. Therefore, we aimed to categorize patients with HNSCC based on LLPS-related genes and explored their multidimensional heterogeneity.

Methods

We integrated the transcriptomic data of 3,541 LLPS-related genes to assess the LLPS patterns in 501 patients with HNSCC within The Cancer Genome Atlas cohort. Subsequently, we explored the differences among the three LLPS subtypes using multi-omics analysis. We also developed an LLPS-related prognostic risk signature (LPRS) to facilitate personalized and integrative assessments and then screened and validated potential therapeutic small molecule compounds targeting HNSCC via experimental analyses.

Result

By analyzing the expression profiles of 85 scaffolds, 355 regulators, and 3,101 clients of LLPS in HNSCC, we identified three distinct LLPS subtypes: LS1, LS2, and LS3. We confirmed notable differences among these subtypes in terms of prognosis, functional enrichment, genomic alterations, TIME patterns, and responses to immunotherapy. Additionally, we developed the LPRS, a prognostic signature for personalized integrative assessments, which demonstrated strong predictive capability for HNSCC prognosis across multiple cohorts. The LPRS also showed significant correlations with the clinicopathological features and TIME patterns in HNSCC patients. Furthermore, the LPRS effectively predicted responses to immune checkpoint inhibitor therapy and facilitated the screening of potential small-molecule compounds for treating HNSCC patients.

Conclusion

This study presents a new classification system for HNSCC patients grounded in LLPS. The LPRS developed in this research offers improved personalized prognosis and could optimize immunotherapy strategies for HNSCC.