bluuebunny/crossref_metadata_2025 huggingface dataset:
Incorporation of CENP-A/CID into centromeres during early Drosophila embryogenesis does not require RNA polymerase II–mediated transcription
Authors: Ghosh Samadri , Lehner Christian F.
Published: January 2022

In many species, centromere identity is specified epigenetically by special nucleosomes containing a centromere-specific histone H3 variant, designated as CENP-A in humans and CID in Drosophila melanogaster. After partitioning of centromere-specific nucleosomes onto newly replicated sister centromeres, loading of additional CENP-A/CID into centromeric chromatin is required for centromere maintenance in proliferating cells. Analyses with cultured cells have indicated that transcription of centromeric DNA by RNA polymerase II is required for deposition of new CID into centromere chromatin. However, a dependence of centromeric CID loading on transcription is difficult to reconcile with the notion that the initial embryonic stages appear to proceed in the absence of transcription in Drosophila, as also in many other animal species. To address the role of RNA polymerase II–mediated transcription for CID loading in early Drosophila embryos, we have quantified the effects of alpha-amanitin and triptolide on centromeric CID-EGFP levels. Our analyses demonstrate that microinjection of these two potent inhibitors of RNA polymerase II–mediated transcription has at most a marginal effect on centromeric CID deposition during progression through the early embryonic cleavage cycles. Thus, we conclude that at least during early Drosophila embryogenesis, incorporation of CID into centromeres does not depend on RNA polymerase II–mediated transcription.
Gender-specific differences in haemostatic parameters and their influence on blood loss in bimaxillary surgery
Authors: Schwaiger Michael , Edmondson Sarah-Jayne , Rabensteiner Jasmin , Prüller Florian , Gary Thomas , Zemann Wolfgang , Wallner Jürgen
Published: January 2022

Objective

The objectives of this prospective cohort study were to establish gender-related differences in blood loss and haemostatic profiles associated with bimaxillary surgery. In addition, we aimed to identify if any gender differences could be established which might help predict blood loss volume.

Materials and methods

Fifty-four patients (22 males; 32 females) undergoing bimaxillary surgery for skeletal dentofacial deformities were eligible for inclusion. Blood samples were taken 1 day preoperatively and 48 h postoperatively for detailed gender-specific coagulation analysis incorporating global coagulation assays (endogenous thrombin potential) and specific coagulation parameters. Blood loss was measured at two different time points: (1) the end of surgery, visible intraoperative blood loss (IOB) using ‘subtraction method’; and (2) 48 h postoperatively perioperative bleeding volume (CBL-48 h) using ‘haemoglobin-balance method’ and Nadler’s formula. Correlation and regression analyses were performed to identify relevant parameters affecting the amount of blood loss.

Results

Significant differences in IOB and CBL-48 h were observed (p < 0.001). Men had higher IOB versus women, lacking statistical significance (p = 0.056). In contrast, men had significantly higher CLB-48 h (p = 0.019). Reduced CBL-48 h was shown to be most closely associated with the level of Antithrombin-III being decreased in females.

Conclusions

Male gender is associated with higher IOB and CBL-48 compared with females. Gender does not affect IOB regarding haemostatic profile but does correlate strongly with procedure length. Conversely, CBL-48 is closely associated with gender-specific imbalances in the anticoagulant system.

Clinical relevance

Knowledge of gender-related differences will help clinicians establish predictive factors regarding excessive blood loss in orthognathic surgery and identify at-risk patients.
Systematic visual analysis of groundwater hydrographs: potential benefits and challenges
Authors: Barthel Roland , Haaf Ezra , Nygren Michelle , Giese Markus
Published: January 2022

Visual analysis of time series in hydrology is frequently seen as a crucial step to becoming acquainted with the nature of the data, as well as detecting unexpected errors, biases, etc. Human eyes, in particular those of a trained expert, are well suited to recognize irregularities and distinct patterns. However, there are limits as to what the eye can resolve and process; moreover, visual analysis is by definition subjective and has low reproducibility. Visual inspection is frequently mentioned in publications, but rarely described in detail, even though it may have significantly affected decisions made in the process of performing the underlying study. This paper presents a visual analysis of groundwater hydrographs that has been performed in relation to attempts to classify groundwater time series as part of developing a new concept for prediction in data-scarce groundwater systems. Within this concept, determining the similarity of groundwater hydrographs is essential. As standard approaches for similarity analysis of groundwater hydrographs do not yet exist, different approaches were developed and tested. This provided the opportunity to carry out a comparison between visual analysis and formal, automated classification approaches. The presented visual classification was carried out on two sets of time series from central Europe and Fennoscandia. It is explained why and where visual classification can be beneficial but also where the limitations and challenges associated with the approach lie. It is concluded that systematic visual analysis of time series in hydrology, despite its subjectivity and low reproducibility, should receive much more attention.
Industrial metabolism and territorial development of the Maurienne Valley (France)
Authors: Talandier Magali , Donsimoni Myriam
Published: January 2022

The globalisation of exchanges has resulted in excessive growth of material and immaterial flows. The disconnection among the supply, production, decision-making and consumption sites generates new spatial interdependencies. It determines local socio-economic dynamics and affects ecosystems. In this context, the question arises if territorial capability—“localized collective capacity to serve territorial development”—influences, from local level, these globalised flows systems. By combining territorial economic principles and territorial ecology approaches, we study the industrial metabolism of the Maurienne valley in France. The Maurienne case shows how territorial characteristics contribute to the economic resilience in rural areas. The calculation of wealth flows provides information on the local economic base, the weight of industry and its social impact. The analysis of physical flows reveals the materiality of this industry and the dependence on external resources and international companies. It highlights the various pressures and risks on the environment. To deal with these constraints, companies rely on relational and geographic proximities with local subcontractors. These relationships determine both the proper functioning of the local industrial system and the territorial capability to maintain and transform industrial activities. Most rural European territories experience the same industrial issues and environmental challenges. Therefore, this study offers new research perspectives to better understand and promote ecological transition in old and often rural industrial areas.
The adoption problem is a matter of fit: tracing the travel of pruning practices from research to farm in Ghana’s cocoa sector
Authors: Obeng Adomaa Faustina , Vellema Sietze , Slingerland Maja , Asare Richard
Published: January 2022

Good Agricultural Practices (GAPs) are central to sustainability standards and certification programmes in the global cocoa chain. Pruning is one of the practices promoted in extension services associated with these sustainability efforts. Yet concerns exist about the low adoption rate of these GAPs by smallholder cocoa farmers in Ghana. A common approach to addressing this challenge is based on creating enabling conditions and offering appropriate incentives. We use the concepts of inscription and affordance to trace the vertically coordinated travel of recommended pruning from research to extension and farming sites, and to describe how pruning is carried out differently at each site. Our analysis suggests that enactments of pruning at the extension site reduce the number of options and space for interactions, and this constrains making the practice meaningful to farmers’ repertoires. The conventions guiding and legitimising actions at this site, reinforced by sustainability standards, certification schemes and associated inspections and audits, favour standardised recommendations and consequently narrow room for context-specific diagnostics and adaptions. Therefore, we reframe the adoption problem as a matter of fit between different sites in the ‘agricultural research value chain’ embedded in the operational cocoa chain. Our contribution problematises the dominant framing of low adoption and highlights that the movement of pruning and the sequential enactment at different sites constrain the affordances available for rendering the practice meaningful to farmers’ repertoires. Consequently, addressing the low uptake of GAPs requires institutional work towards conventions that can construct a fit between sites along the agricultural research value chain.
Leading new, deeper forms of collaborative cultures: Questions and pathways
Authors: Azorín Cecilia , Fullan Michael
Published: January 2022

The pandemic has made deeper problems more transparent and has stimulated many to realize that there may be an opportunity over the next period to pursue much needed innovations in learning. In this essay we describe the ways in which the pandemic has provided the conditions for new human development that joins two powerful forces: the pulsar model which elevates human potential with respect to student learning, and new, deeper forms of collaboration that have long eluded those interested in system change. In this article we show how ‘spirit work’ and collaboration can combine to develop schools systems that are essential for coping with the new post-pandemic conditions facing humanity. We also identify spinoff opportunities arising from the pandemic, and a corresponding pressure that could generate more widespread system improvement designed to improve learning for all, including advances in both equity and excellence.
The impact of reduced tillage and distance to field margin on predator functional diversity
Authors: Jacobsen Stine K. , Sigsgaard Lene , Johansen Anna B. , Thorup-Kristensen Kristian , Jensen Per M.
Published: January 2022

Introduction

Agricultural intensification results in biodiversity loss through land conversion and management practices which negatively impact arthropods. The abundance and diversity of ground-dwelling predators, e.g. ground beetles (Coleoptera: Carabidae) and spiders (Araneae), are negatively affected by soil disturbances such as tillage. Reducing soil disturbances can potentially conserve arthropod populations in the field and reduce the use of chemical pest controls. The present study investigated the ground-dwelling predatory community using pitfall traps in cereal fields with three different levels of soil disturbance: conventional tillage, reduced tillage and no tillage under Conservation Agriculture management, in 2018 and 2019. Pitfall traps were placed in transects from the field margins. Overall, the activity-density of ground-dwelling predators was higher in fields with minimum soil disturbance and generally declined with increased distance to semi-natural habitats. Functional diversity, expressed by the body size of ground beetles, was also affected by soil disturbances; large ground beetles more consistently occurred in CA, while few or none of the largest ground beetles were found in RT and CT. A higher sample-heterogeneity in less disturbed fields was indicated by a more variable median and higher skewness in the number of predators in those fields. In 2019 only, species diversity was higher along field edges bordering semi-natural habitats when compared to the cropped area. Our results show that reduced tillage supports predator arthropod communities at a local scale: It also bolsters agro-ecosystem resilience by promoting a higher activity-density and by increasing the heterogeneity and functional diversity of ground-dwelling predators.

Implications for insect conservation

The results obtained in the present study show that soil disturbances significantly influence arthropod abundance and diversity. Conservation of epigeic natural enemies in the agricultural landscape is improved by reducing soil-disturbing events such as tillage.
The BCS Critical Temperature at High Density
Authors: Henheik Joscha
Published: January 2022

We investigate the BCS critical temperature $$T_c$$

T
c

in the high-density limit and derive an asymptotic formula, which strongly depends on the behavior of the interaction potential V on the Fermi-surface. Our results include a rigorous confirmation for the behavior of $$T_c$$

T
c

at high densities proposed by Langmann et al. (Phys Rev Lett 122:157001, 2019) and identify precise conditions under which superconducting domes arise in BCS theory.
Young People with Intellectual Disability and Their Experiences with Intimate Relationships: a Follow-up Analysis of Parents’ and Caregivers’ Perspectives
Authors: Retznik Laura , Wienholz Sabine , Höltermann Annelen , Conrad Ines , Riedel-Heller Steffi G.
Published: January 2022

Background

Research shows that people with intellectual disability both want and benefit from relationships. Caregivers play an important role in the development and maintenance of these relationships. Barriers to relationships include lack of privacy, inadequate sex education, and lack of public transportation.

Method

The sample included 42 primary caregivers of young people with intellectual disability aged 14 to 25. Interviews were evaluated with the qualitative content analysis.

Results

Two-thirds of the caregivers reported the young people having had at least one relationship. The caregivers tended to minimize the importance of the young people’s relationships and reported, in equal measure, both positive and negative relationship skills. Caregivers described limited choice in terms of partner selection, lack of social opportunities, and low quality couple time. Parents face the detachment process with ambivalence and wish for a stable partnership for their children in the future.

Discussion

Young people with intellectual disability face barriers and limitations regarding intimate relationships. Some young people may keep their partnerships secret to avoid possible restrictions from their caregivers. Their time together is quantitatively high, but qualitatively poor due to a lack of transportation and inclusive services. Caregivers need to support the young people’s autonomy and take their relationships more seriously.
Density-functional-theory predictions of mechanical behaviour and thermal properties as well as experimental hardness of the Ga-bilayer Mo2Ga2C
Authors: Qi Xinxin , Yin Weilong , Jin Sen , Zhou Aiguo , He Xiaodong , Song Guangping , Zheng Yongting , Bai Yuelei
Published: January 2022

Mo2Ga2C is a new MAX phase with a stacking Ga-bilayer as well as possible unusual properties. To understand this unique MAX phase structure and promote possible future applications, the structure, chemical bonding, and mechanical and thermodynamic properties of Mo2Ga2C were investigated by first-principles. Using the “bond stiffness” model, the strongest covalent bonding (1162 GPa) was formed between Mo and C atoms in Mo2Ga2C, while the weakest Ga-Ga (389 GPa) bonding was formed between two Ga-atomic layers, different from other typical MAX phases. The ratio of the bond stiffness of the weakest bond to the strongest bond (0.33) was lower than 1/2, indicating the high damage tolerance and fracture toughness of Mo2Ga2C, which was confirmed by indentation without any cracks. The high-temperature heat capacity and thermal expansion of Mo2Ga2C were calculated in the framework of quasi-harmonic approximation from 0 to 1300 K. Because of the metal-like electronic structure, the electronic excitation contribution became more significant with increasing temperature above 300 K.
Si-based polymer-derived ceramics for energy conversion and storage
Authors: Wen Qingbo , Qu Fangmu , Yu Zhaoju , Graczyk-Zajac Magdalena , Xiong Xiang , Riedel Ralf
Published: January 2022

Since the 1960s, a new class of Si-based advanced ceramics called polymer-derived ceramics (PDCs) has been widely reported because of their unique capabilities to produce various ceramic materials (e.g., ceramic fibers, ceramic matrix composites, foams, films, and coatings) and their versatile applications. Particularly, due to their promising structural and functional properties for energy conversion and storage, the applications of PDCs in these fields have attracted much attention in recent years. This review highlights the recent progress in the PDC field with the focus on energy conversion and storage applications. Firstly, a brief introduction of the Si-based polymer-derived ceramics in terms of synthesis, processing, and microstructure characterization is provided, followed by a summary of PDCs used in energy conversion systems (mainly in gas turbine engines), including fundamentals and material issues, ceramic matrix composites, ceramic fibers, thermal and environmental barrier coatings, as well as high-temperature sensors. Subsequently, applications of PDCs in the field of energy storage are reviewed with a strong focus on anode materials for lithium and sodium ion batteries. The possible applications of the PDCs in Li-S batteries, supercapacitors, and fuel cells are discussed as well. Finally, a summary of the reported applications and perspectives for future research with PDCs are presented.
Delirium and subsyndromal delirium are associated with the long-term risk of death after ischaemic stroke
Authors: Klimiec-Moskal Elzbieta , Slowik Agnieszka , Dziedzic Tomasz
Published: January 2022

Background

Post-stroke delirium has a negative impact on functional outcome. We explored if there is any association between delirium, subsyndromal delirium and long-term mortality after ischaemic stroke and transient ischaemic attack.

Methods

We included 564 patients with ischaemic stroke or transient ischaemic attack. We assessed symptoms of delirium during the first 7 days after admission. We used Cox proportional hazards models to analyse all-cause mortality during the first 5 years after stroke.

Results

We diagnosed delirium in 23.4% and subsyndromal delirium in 10.3% of patients. During the follow-up, 72.7% of patients with delirium, 51.7% of patients with subsyndromal delirium and 22.7% of patients without delirious symptoms died (P < 0.001). Patients with subsyndromal delirium and delirium had higher risk of death in the multivariate analysis (HR 1.72, 95% CI 1.11–2.68, P = 0.016 and HR 3.30, 95% CI 2.29–4.76, P < 0.001, respectively).

Conclusions

Post-stroke delirium is associated with long-term mortality. Patients with subsyndromal delirium are at the intermediate risk of death.
Valuation and Returns of Drug Development Companies: Lessons for Bioentrepreneurs and Investors
Authors: Michaeli Daniel Tobias , Yagmur Hasan Basri , Achmadeev Timur , Michaeli Thomas
Published: January 2022

Objectives

This study evaluates the association of Biopharma company valuation with the lead drug’s development stage, orphan status, number of indications, and disease area. We also estimated annual returns Bioentrepreneurs and investors can expect from founding and investing in drug development ventures.

Methods

SDC Thomson Reuter and S&P Capital IQ were screened for majority acquisitions of US and EU Biopharma companies developing new molecular entities for prescription use (SIC code: 2834). Acquisition data were complemented with drug characteristics extracted from clinicaltrials.gov, the US Food and Drug Administration (FDA), and deal announcements. Thereafter, company valuations were combined with previously published clinical development periods alongside orphan-, indication-, and disease-specific success rates to estimate annual returns for investments in drug developing companies.

Results

Based on a sample of 311 Biopharma acquisitions from 2005 to 2020, companies developing orphan, multi-indication, and oncology drugs were valued significantly higher than their peers during later development stages (p < 0.05). We also estimated significantly higher returns for shareholders of companies with orphan relative to non-orphan-designated lead drugs from Phase 1 to FDA approval (46% vs. 12%, p < 0.001). Drugs developed across multiple indications also provided higher returns than single-indication agents from Pre-Clinic to FDA approval (21% vs. 11%, p < 0.001). Returns for oncology drugs exceeded other disease areas (26% vs. 8%, p < 0.001).

Conclusions

Clinical and economic conditions surrounding orphan-designated drugs translate to a favorable financial risk-return profile for Bioentrepreneurs and investors. Bioentrepreneurs must be aware of the upside real option value their multi-indication drug could offer when negotiating acquisition or licensing agreements.
Fissures in EU Citizenship
Authors: Steinfeld Martin
Published: January 2022

This book argues that core concepts in EU citizenship law are riddled with latent fissures traceable back to the earliest case law on free movement of persons, and that later developments simply compounded such defects. By looking at these defects, not only could Brexit have been predicted, but it could also have been foreseen that unchecked problems with EU citizenship would potentially lead to its eventual dismantling during an era of widespread populism and considerable challenges to further integration. Using a critical constructivist approach, the author painstakingly outlines the 'temple' of citizenship from its foundations upwards, and offers a deconstruction of concepts such as 'worker', the role of non-economic actors, the principle of equal treatment, and utterances of citizenship. In identifying inherent fissures in the concept of solidarity and post national identification, this book poses critical questions and argues that we need to reconstruct EU citizenship from the bottom up.
Centrifugal spinning of viscoelastic nanofibres
Authors: Noroozi S. , Arne W. , Larson R.G. , Taghavi S.M.
Published: January 2022

The centrifugal spinning method is a recently invented technique to extrude polymer melts/solutions into ultra-fine nanofibres. Here, we present a superior integrated string-based mathematical model, to quantify the nanofibre fabrication performance in the centrifugal spinning process. Our model enables us to analyse the critical flow parameters covering an extensive range, by incorporating the angular momentum equations, the Giesekus viscoelastic constitutive model, the air-to-fibre drag effects and the energy equation into the string model equations. Using the model, we can analyse the dynamic behaviour of polymer melt/solution jets through the dimensionless flow parameters, namely, the Rossby ($Rb$), Reynolds ($Re$), Weissenberg ($Wi$), Weber ($We$), Froude ($Fr$), air Péclet ($Pe^*$) and air Reynolds ($Re^*$) numbers as well as the viscosity ratio ($\delta _s$), corresponding to rotational, inertial, viscous, viscoelastic, surface tension, gravitational, air thermal diffusivity, aerodynamic and viscosity ratio effects. We find that the nonlinear rheology remarkably affects the fibre trajectory, radius and normal stresses. Increasing $Wi$ leads to a thicker fibre, whereas increasing $\delta _s$ shows an opposite trend. In addition, by increasing $Wi$, the fibre curvature is enhanced, causing the fibre to spiral closer to the rotation centre.
Ice breaking by a high-speed water jet impact
Authors: Yuan G.-Y. , Ni B.-Y. , Wu Q.-G. , Xue Y.-Z. , Han D.-F.
Published: January 2022

Ice breaking has become one of the main problems faced by ships and other equipment operating in an ice-covered water region. New methods are always being pursued and studied to improve ice-breaking capabilities and efficiencies. Based on the strong damage capability, a high-speed water jet impact is proposed to be used to break an ice plate in contact with water. A series of experiments of water jet impacting ice were performed in a transparent water tank, where the water jets at tens of metres per second were generated by a home-made device and circular ice plates of various thicknesses and scales were produced in a cold room. The entire evolution of the water jet and ice was recorded by two high-speed cameras from the top and front views simultaneously. The focus was the responses of the ice plate, such as crack development and breakup, under the high-speed water jet loads, which involved compressible pressure

${P_1}$

and incompressible pressure

${P_2}$

. According to the main cause and crack development sequence, it was found that the damage of the ice could be roughly divided into five patterns. On this basis, the effects of water jet strength, ice thickness, ice plate size and boundary conditions were also investigated. Experiments validated the ice-breaking capability of the high-speed water jet, which could be a new auxiliary ice-breaking method in the future.
The Chinese Latin Alphabet: A Revolutionary Script in the Global Information Age
Authors: Kuzuoğlu Uluğ
Published: January 2022

This article rethinks the history of Chinese script reforms and proposes a new genealogy for the Chinese Latin Alphabet (CLA), invented in 1931 by Chinese and Russian revolutionaries in the Soviet Union. Situating script reforms within a global information age that emerged out of the nineteenth-century communications revolution, the article historicizes the CLA within a technologically and ideologically contrived Sino-Soviet space. In particular, it shows the intimate links between the CLA and the Unified New Turkic Alphabet (UNTA), which grew out of a latinization movement based in Baku, Azerbaijan. The primary purpose of the UNTA was to latinize the Arabic script of the Turkic people living in Soviet Central Asia, but it was immediately exported to the non-Turkic world as well in an effort to latinize languages across Eurasia and ignite revolutionary internationalism. This article investigates the forgotten figures involved in carrying the Latin alphabet from Baku to Shanghai and offers a new framework to scrutinize the history of language, scripts, and knowledge production across Eurasia.
The road to the 1980s write-downs of sovereign debt
Authors: Truman Edwin M.
Published: January 2022

The Latin American debt crisis consumed the 1980s and was not restricted to Latin America. Starting from the August 1982 Mexican weekend, the crisis had three phases: Concerted Lending (1982-5), Baker Plan (1985-9) and Brady Plan (1989 to mid 1990s). This article describes the evolution of the debt strategy and the road to embracing debt write-downs at the end of the decade. In the absence of an external coordinating mechanism, four groups of parties had to reach agreement on any change in the strategy: the borrowing countries, their commercial bank lenders, the home-country authorities of those lenders, and the International Monetary Fund as the principal international institution. Each group could effectively veto any change in the strategy. This need for consensus is lesson number one from the 1980s for today. Lesson number two is that political economy aspects dictated that the strategy be implemented on a case-by-case basis. The article concludes with an application of these lessons to a similar, but even more global, potential debt crisis in the wake of the COVID pandemic.
Crimes and sentences in individuals with intellectual disability in a forensic psychiatric context: a register-based study
Authors: Edberg H. , Chen Q. , Andiné P. , Larsson H. , Hirvikoski T.
Published: January 2022

Aims

To study associations between intellectual disability (ID) and sexual and violent offending among individuals subject to pre-trial forensic psychiatric assessment. To investigate sentences following pre-trial forensic psychiatric assessment in offenders with and without ID.

Methods

A population-based observational study using data from pre-trial forensic psychiatric assessments in Sweden (1997–2013), the Swedish National Crime Register and several other Swedish national registers. The study population consisted of 7450 offenders (87% men, 13% women) who were subject to forensic psychiatric assessment in 1997–2013, of whom 481 (6.5%) were clinically assessed as having ID.

Results

ID offenders were more likely than non-ID offenders to have a sexual crime as an index crime [26.2v. 11.5%, adjusted odds ratio (OR) 2.7, 95% confidence interval (CI) 2.02–3.58] as well as previous convictions regarding sexual offending (10.4v. 5.6%, adj OR 2.3, 95% CI 1.70–3.12). These associations were restricted to male offenders; sexual offending was uncommon among women. Comorbid attention-deficit hyperactivity disorder reduced the association between ID and sexual offending (adj OR 2.7v. 3.1,p= 0.017), while comorbid autism spectrum disorder had no significant influence on the association (adj OR 2.7v. 3.0,p= 0.059). Violent crime was equally common among ID and non-ID offenders. Offenders with ID were more likely than non-ID offenders to be sentenced to forensic psychiatric care or community sanctions and measures (such as probation, conditional sentences or fines) than to prison; however, 15% of individuals who received an ID diagnosis during the forensic psychiatric assessment were sentenced to prison. Previous criminal convictions, concurrent antisocial personality disorders and substance use disorders were associated with a higher probability of a prison sentence among offenders with ID.

Conclusions

Sexual crime is overrepresented among offenders with ID compared to offenders with other mental disorders than ID in forensic psychiatric contexts. ID offenders become subject to forensic psychiatric care and forensic psychiatric services need evidence-based treatment programmes for offenders with ID. In addition, there is a need for early intervention strategies suitable for disability services and special education schools, in order to address the complex needs of individuals with ID and prevent sexual and violent offending.
Music Research and the Sound Archive: A Meditation on Ethnomusicological Engagement with Collection-Oriented Research and Re(p)(m)atriation
Authors: GIROUX MONIQUE
Published: January 2022

In this article, I address collecting and re(p)(m)atriation as research orientations. I draw on examples from Métis music to situate the impact of collection-oriented research, to interrogate my own practice as a Métis-music scholar, and to point to possibilities for the future. In presenting a history of collecting alongside an overview of re(p)(m)atriation, I offer readers an opportunity to meditate on the pervasiveness of collection-oriented research and how we might create a new ethnomusicology—meditations encouraged through poetic expressions. I suggest that twenty-first century ethnomusicology needs to turn towards rematriation, not only as an act of returning artifacts, but also as a way of orienting our work as scholars.
Crustal Structure of the Hikurangi Margin From SHIRE Seismic Data and the Relationship Between Forearc Structure and Shallow Megathrust Slip Behavior
Authors: Bassett Dan , Arnulf Adrien , Henrys Stuart , Barker Dan , van Avendonk Harm , Bangs Nathan , Kodaira Shuichi , Seebeck Hannu , Wallace Laura , Gase Andrew , Luckie Thomas , Jacobs Katie , Tozer Brook , Arai Ryuta , Okaya David , Mochizuki Kimi , Fujie Gou , Yamamoto Yojiro
Published: January 2022

Marine multichannel and wide‐angle seismic data constrain crustal structure along a 530 km margin‐parallel transect of the Hikurangi subduction zone. The subducting Hikurangi Plateau crust (VP 5.0–7.4 km/s) is ∼1 km thicker (11 ± 1 km) and mantle velocities are ∼0.2 km/s higher (VP 8.3–8.5 km/s) beneath south/central Hikurangi relative to north Hikurangi. In the overthrusting plate, an abrupt 0.5 km/s south‐to‐north reduction in forearc wavespeeds occurs in concert with a change in seismic reflection character. We analyze legacy seismic data to show that the forearc transition likely reflects lateral variability in the updip extent of the Torlesse Backstop. Furthermore, we map this unit along‐strike and note a broad correlation between the backstop and down‐dip extent of shallow slow‐slip. We propose that the geological architecture of the overthrusting plate contributes to spatial variability in the location of shallow frictional transitions along the Hikurangi margin, impacting both seismic and tsunami hazard.
Biocrust “Probiotics” Can Aid Dryland Restoration Efforts
Authors: Smith Derek
Published: January 2022

Bacteria can speed up the growth of biocrust-forming organisms in nurseries, providing more material for restoration of degraded dryland soil.
Weaker connectivity in resting state networks is associated with disinhibited eating in older adults
Authors: Brennan Anthony , Marstaller Lars , Burianová Hana , Benton David , Hanley Claire J. , Newstead Simon , Young Hayley A.
Published: January 2022

Background/objectives

Obesity affects more than forty percent of adults over the age of sixty. Aberrant eating styles such as disinhibition have been associated with the engagement of brain networks underlying executive functioning, attentional control, and interoception. However, these effects have been exclusively studied in young samples overlooking those most at risk of obesity related harm.

Methods

Here we assessed associations between resting-state functional connectivity and disinhibited eating (using the Three Factor Eating Questionnaire) in twenty-one younger (aged 19–34 years, BMI range: 18–31) and twenty older (aged 60–73 years, BMI range: 19–32) adults matched for BMI. The Alternative Healthy Eating Index was used to quantify diet quality.

Results

Older, compared to younger, individuals reported lower levels of disinhibited eating, consumed a healthier diet, and had weaker connectivity in the frontoparietal (FPN) and default mode (DMN) networks. In addition, associations between functional connectivity and eating behaviour differed between the two age groups. In older adults, disinhibited eating was associated with weaker connectivity in the FPN and DMN––effects that were absent in the younger sample. Importantly, these effects could not be explained by differences in habitual diet.

Conclusions

These findings point to a change in interoceptive signalling as part of the ageing process, which may contribute to behavioural changes in energy intake, and highlight the importance of studying this under researched population.
Blockade of beta-adrenergic receptors reduces cancer growth and enhances the response to anti-CTLA4 therapy by modulating the tumor microenvironment
Authors: Fjæstad Klaire Yixin , Rømer Anne Mette Askehøj , Goitea Victor , Johansen Astrid Zedlitz , Thorseth Marie-Louise , Carretta Marco , Engelholm Lars Henning , Grøntved Lars , Junker Niels , Madsen Daniel Hargbøl
Published: January 2022

The development of immune checkpoint inhibitors (ICI) marks an important breakthrough of cancer therapies in the past years. However, only a limited fraction of patients benefit from such treatments, prompting the search for immune modulating agents that can improve the therapeutic efficacy. The nonselective beta blocker, propranolol, which for decades has been prescribed for the treatment of cardiovascular conditions, has recently been used successfully to treat metastatic angiosarcoma. These results have led to an orphan drug designation by the European Medicines Agency for the treatment of soft tissue sarcomas. The anti-tumor effects of propranolol are suggested to involve the reduction of cancer cell proliferation as well as angiogenesis. Here, we show that oral administration of propranolol delays tumor progression of MCA205 fibrosarcoma model and MC38 colon cancer model and increases the survival rate of tumor bearing mice. Propranolol works by reducing tumor angiogenesis and facilitating an anti-tumoral microenvironment with increased T cell infiltration and reduced infiltration of myeloid-derived suppressor cells (MDSCs). Using T cell deficient mice, we demonstrate that the full anti-tumor effect of propranolol requires the presence of T cells. Flow cytometry-based analysis and RNA sequencing of FACS-sorted cells show that propranolol treatment leads to an upregulation of PD-L1 on tumor associated macrophages (TAMs) and changes in their chemokine expression profile. Lastly, we observe that the co-administration of propranolol significantly enhances the efficacy of anti-CTLA4 therapy. Our results identify propranolol as an immune modulating agent, which can improve immune checkpoint inhibitor therapies in soft tissue sarcoma patients and potentially in other cancers.
Linc01133 contributes to gastric cancer growth by enhancing YES1-dependent YAP1 nuclear translocation via sponging miR-145-5p
Authors: Sun Yujing , Tian Yuan , He Junyi , Tian Yaru , Zhang Guohao , Zhao Ruinan , Zhu Wen-jie , Gao Peng
Published: January 2022

The long intergenic non-coding RNA linc01133 is reported to be oncogenic in various malignancies. However, the role and mechanism of linc01133 in regulating gastric cancer growth is still not clear. In the present study, we found that linc01133 was significantly upregulated in gastric cancer tissues compared to non-tumorous gastric tissues. Linc01133 over-expression significantly correlated with tumor size and tumor differentiation in gastric cancer patients. The expression of linc01133 was regulated by c-Jun and c-Fos collaboratively. In both in vitro and in vivo studies, linc01133 was shown to promote gastric cancer cell growth. Linc01133 localized in the cytoplasm and functioned as an endogenous competing RNA of miR-145-5p to upregulate the expression of YES1, which was proved to be the target gene of miR-145-5p. By promoting YES1-dependent YAP1 nuclear translocation, linc01133 upregulated the expression of the key cell cycle regulators CDK4, CDK6 and cyclin D1 to promote G1-S phase transition. Thus, our study unveiled the function and mechanism of linc01133 regulating cell cycle progression in gastric cancer.
The immune-suppressive landscape in lepromatous leprosy revealed by single-cell RNA sequencing
Authors: Mi Zihao , Wang Zhenzhen , Xue Xiaotong , Liu Tingting , Wang Chuan , Sun Lele , Yu Gongqi , Zhang Yuan , Shi Peidian , Sun Yonghu , Yang Yongliang , Ma Shanshan , Wang Zhe , Yu Yueqian , Liu Jianjun , Liu Hong , Zhang Furen
Published: January 2022

Lepromatous leprosy (L-LEP), caused by the massive proliferation of Mycobacterium leprae primarily in macrophages, is an ideal disease model for investigating the molecular mechanism of intracellular bacteria evading or modulating host immune response. Here, we performed single-cell RNA sequencing of both skin biopsies and peripheral blood mononuclear cells (PBMCs) of L-LEP patients and healthy controls. In L-LEP lesions, we revealed remarkable upregulation of APOE expression that showed a negative correlation with the major histocompatibility complex II gene HLA-DQB2 and MIF, which encodes a pro-inflammatory and anti-microbial cytokine, in the subset of macrophages exhibiting a high expression level of LIPA. The exhaustion of CD8+ T cells featured by the high expression of TIGIT and LAG3 in L-LEP lesions was demonstrated. Moreover, remarkable enhancement of inhibitory immune receptors mediated crosstalk between skin immune cells was observed in L-LEP lesions. For PBMCs, a high expression level of APOE in the HLA-DRhighFBP1high monocyte subset and the expansion of regulatory T cells were found to be associated with L-LEP. These findings revealed the primary suppressive landscape in the L-LEP patients, providing potential targets for the intervention of intracellular bacteria caused persistent infections.
Gut microbiota promotes cholesterol gallstone formation by modulating bile acid composition and biliary cholesterol secretion
Authors: Hu Hai , Shao Wentao , Liu Qian , Liu Ning , Wang Qihan , Xu Jin , Zhang Xin , Weng Zhenkun , Lu Qifan , Jiao Long , Chen Chaobo , Sun Haidong , Jiang Zhaoyan , Zhang Xiaoping , Gu Aihua
Published: January 2022

Cholesterol gallstone disease is a worldwide common disease. Cholesterol supersaturation in gallbladder bile is the prerequisite for its pathogenesis, while the mechanism is not completely understood. In this study, we find enrichment of gut microbiota (especially Desulfovibrionales) in patients with gallstone disease. Fecal transplantation of gut microbiota from gallstone patients to gallstone-resistant strain of mice can induce gallstone formation. Carrying Desulfovibrionales is associated with enhanced cecal secondary bile acids production and increase of bile acid hydrophobicity facilitating intestinal cholesterol absorption. Meanwhile, the metabolic product of Desulfovibrionales, H2S increase and is shown to induce hepatic FXR and inhibit CYP7A1 expression. Mice carrying Desulfovibrionales present induction of hepatic expression of cholesterol transporters Abcg5/g8 to promote biliary secretion of cholesterol as well. Our study demonstrates the role of gut microbiota, Desulfovibrionales, as an environmental regulator contributing to gallstone formation through its influence on bile acid and cholesterol metabolism.
Preparative-scale synthesis of nonacene
Authors: Jančařík Andrej , Holec Jan , Nagata Yuuya , Šámal Michal , Gourdon Andre
Published: January 2022

During the last years we have witnessed progressive evolution of preparation of acenes with length up to dodecacene by on-surface synthesis in ultra-high vacuum or generation of acenes up to decacene in solid matrices at low temperatures. While these protocols with very specific conditions produce the acenes in amount of few molecules, the strategies leading to the acenes in large quantities dawdle behind. Only recently and after 70 years of synthetic attempts, heptacene has been prepared in bulk phase. However, the preparative scale synthesis of higher homologues still remains a formidable challenge. Here we report the preparation and characterisation of nonacene and show its excellent thermal and in-time stability.
A bimodal distribution of haze in Pluto’s atmosphere
Authors: Fan Siteng , Gao Peter , Zhang Xi , Adams Danica J. , Kutsop Nicholas W. , Bierson Carver J. , Liu Chao , Yang Jiani , Young Leslie A. , Cheng Andrew F. , Yung Yuk L.
Published: January 2022

Pluto, Titan, and Triton make up a unique class of solar system bodies, with icy surfaces and chemically reducing atmospheres rich in organic photochemistry and haze formation. Hazes play important roles in these atmospheres, with physical and chemical processes highly dependent on particle sizes, but the haze size distribution in reducing atmospheres is currently poorly understood. Here we report observational evidence that Pluto’s haze particles are bimodally distributed, which successfully reproduces the full phase scattering observations from New Horizons. Combined with previous simulations of Titan’s haze, this result suggests that haze particles in reducing atmospheres undergo rapid shape change near pressure levels ~0.5 Pa and favors a photochemical rather than a dynamical origin for the formation of Titan’s detached haze. It also demonstrates that both oxidizing and reducing atmospheres can produce multi-modal hazes, and encourages reanalysis of observations of hazes on Titan and Triton.
C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
Authors: Park Inhye , Goddard Michael E. , Cole Jennifer E. , Zanin Natacha , Lyytikäinen Leo-Pekka , Lehtimäki Terho , Andreakos Evangelos , Feldmann Marc , Udalova Irina , Drozdov Ignat , Monaco Claudia
Published: January 2022

Macrophages are integral to the pathogenesis of atherosclerosis, but the contribution of distinct macrophage subsets to disease remains poorly defined. Using single cell technologies and conditional ablation via aLysMCre+Clec4a2flox/DTRmouse strain, we demonstrate that the expression of the C-type lectin receptor CLEC4A2 is a distinguishing feature of vascular resident macrophages endowed with athero-protective properties. Through genetic deletion and competitive bone marrow chimera experiments, we identify CLEC4A2 as an intrinsic regulator of macrophage tissue adaptation by promoting a bias in monocyte-to-macrophage in situ differentiation towards colony stimulating factor 1 (CSF1) in vascular health and disease. During atherogenesis, CLEC4A2 deficiency results in loss of resident vascular macrophages and their homeostatic properties causing dysfunctional cholesterol metabolism and enhanced toll-like receptor triggering, exacerbating disease. Our study demonstrates that CLEC4A2 licenses monocytes to join the vascular resident macrophage pool, and that CLEC4A2-mediated macrophage homeostasis is critical to combat cardiovascular disease.
MyoD is a 3D genome structure organizer for muscle cell identity
Authors: Wang Ruiting , Chen Fengling , Chen Qian , Wan Xin , Shi Minglei , Chen Antony K. , Ma Zhao , Li Guohong , Wang Min , Ying Yachen , Liu Qinyao , Li Hu , Zhang Xu , Ma Jinbiao , Zhong Jiayun , Chen Meihong , Zhang Michael Q. , Zhang Yong , Chen Yang , Zhu Dahai
Published: January 2022

The genome exists as an organized, three-dimensional (3D) dynamic architecture, and each cell type has a unique 3D genome organization that determines its cell identity. An unresolved question is how cell type-specific 3D genome structures are established during development. Here, we analyzed 3D genome structures in muscle cells from mice lacking the muscle lineage transcription factor (TF), MyoD, versus wild-type mice. We show that MyoD functions as a “genome organizer” that specifies 3D genome architecture unique to muscle cell development, and that H3K27ac is insufficient for the establishment of MyoD-induced chromatin loops in muscle cells. Moreover, we present evidence that other cell lineage-specific TFs might also exert functional roles in orchestrating lineage-specific 3D genome organization during development.
Robust ultrathin nanoporous MOF membrane with intra-crystalline defects for fast water transport
Authors: Wang Xueling , Lyu Qiang , Tong Tiezheng , Sun Kuo , Lin Li-Chiang , Tang Chuyang Y. , Yang Fenglin , Guiver Michael D. , Quan Xie , Dong Yingchao
Published: January 2022

Rational design of high-performance stable metal–organic framework (MOF) membranes is challenging, especially for the sustainable treatment of hypersaline waters to address critical global environmental issues. Herein, a molecular-level intra-crystalline defect strategy combined with a selective layer thinning protocol is proposed to fabricate robust ultrathin missing-linker UiO-66 (ML-UiO-66) membrane to enable fast water permeation. Besides almost complete salt rejection, high and stable water flux is achieved even under long-term pervaporation operation in hash environments, which effectively addresses challenging stability issues. Then, detailed structural characterizations are employed to identify the type, chemical functionality, and density of intra-crystalline missing-linker defects. Moreover, molecular dynamics simulations shed light on the positive atomistic role of these defects, which are responsible for substantially enhancing structural hydrophilicity and enlarging pore window, consequently allowing ultra-fast water transport via a lower-energy-barrier pathway across three-dimensional sub-nanochannels during pervaporation. Unlike common unfavorable defect effects, the present positive intra-crystalline defect engineering concept at the molecular level is expected to pave a promising way toward not only rational design of next-generation MOF membranes with enhanced permeation performance, but additional water treatment applications.