bluuebunny/crossref_metadata_2025 huggingface dataset:
Super-Resolution Microscopy Reveals a Direct Interaction of Intracellular Mycobacterium tuberculosis with the Antimicrobial Peptide LL-37
Authors: Deshpande Dhruva , Grieshober Mark , Wondany Fanny , Gerbl Fabian , Noschka Reiner , Michaelis Jens , Stenger Steffen
Published: September 2020

The antimicrobial peptide LL-37 inhibits the growth of the major human pathogen Mycobacterium tuberculosis (Mtb), but the mechanism of the peptide–pathogen interaction inside human macrophages remains unclear. Super-resolution imaging techniques provide a novel opportunity to visualize these interactions on a molecular level. Here, we adapt the super-resolution technique of stimulated emission depletion (STED) microscopy to study the uptake, intracellular localization and interaction of LL-37 with macrophages and virulent Mtb. We demonstrate that LL-37 is internalized by both uninfected and Mtb infected primary human macrophages. The peptide localizes in the membrane of early endosomes and lysosomes, the compartment in which mycobacteria reside. Functionally, LL-37 disrupts the cell wall of intra- and extracellular Mtb, resulting in the killing of the pathogen. In conclusion, we introduce STED microscopy as an innovative and informative tool for studying host–pathogen–peptide interactions, clearly extending the possibilities of conventional confocal microscopy.
Vacuum Hot Pressing of Oxide Dispersion Strengthened Ferritic Stainless Steels: Effect of Al Addition on the Microstructure and Properties
Authors: Ganesan Dharmalingam , Sellamuthu Prabhukumar , Prashanth Konda Gokuldoss
Published: September 2020

The present article investigates the fabrication of oxide dispersion strengthened (ODS) ferritic stainless steel (FSS). Three different ODS alloys with three different Al contents were fabricated, where the presence of Al-based oxides play a crucial role in determining the size of the oxide particles. Due to Ostwald ripening, the samples with Al show coarser oxide particles compared to the alloy without Al, which hampers the density of the fabricated samples and, hence, have reduced hardness levels. The present results suggest that the composition of the oxide present in ODS plays a crucial role in determining the properties of these samples.
Key Environmental Factors Controlling Planktonic Foraminiferal and Pteropod Community’s Response to Late Quaternary Hydroclimate Changes in the South Aegean Sea (Eastern Mediterranean)
Authors: Giamali Christina , Kontakiotis George , Koskeridou Efterpi , Ioakim Chryssanthi , Antonarakou Assimina
Published: September 2020

A multidisciplinary study was conducted in order to investigate the environmental factors affecting the planktonic foraminiferal and pteropod communities of the south Aegean Sea. Aspects of the Late Quaternary paleoceanographic evolution were revealed by means of quantitative analyses of planktonic foraminiferal and pteropod assemblages (including multivariate statistical approach; principal component analysis (PCA)), the oxygen (δ18O) and carbon (δ13C) isotopic composition of planktonic foraminifera and related paleoceanographic (planktonic paleoclimatic curve (PPC), productivity (E-index), stratification (S-index), seasonality) indices, extracted by the gravity core KIM-2A derived from the submarine area between Kimolos and Sifnos islands. Focusing on the last ~21 calibrated thousands of years before present (ka BP), cold and eutrophicated conditions were identified during the Late Glacial period (21.1–15.7 ka BP) and were followed by warmer and wetter conditions during the deglaciation phase. The beginning of the Holocene was marked by a climatic amelioration and increased seasonality. The more pronounced environmental changes were identified during the deposition of the sapropel sublayers S1a (9.4–7.7 ka BP) and S1b (6.9–6.4 ka BP), with extremely warm and stratified conditions. Pteropod fauna during the sapropel deposition were recorded for the first time in the south Aegean Sea, suggesting arid conditions towards the end of S1a. Besides sea surface temperature (SST), which shows the highest explanatory power for the distribution of the analyzed fauna, water column stratification, primary productivity, and seasonality also control their communities during the Late Quaternary.
Multiphase Marine Ramjet Using Liquefied Gas as a Source of Bubbles
Authors: Haustein Herman D. , Gany Alon , Elias Ezra
Published: September 2020

An innovative variant of a multiphase marine ramjet is investigated analytically and experimentally. Pressure liquefied gas (LG) is injected, boiling under superheat conditions in the water stream within the propulsion unit, and serving as an on-board bubble source necessary for the ramjet operation. Experiments were conducted in a 10 m diameter tow pool at a speed range of 7–18 m/s (approximately 14–35 knots). For the laboratory concept demonstration and process characterization, two liquefied gases, butane and R134a (synthetic refrigerant gas), were employed. In practical applications, non-polluting LGs such as liquid air may be used. The results reveal the overall performance comparable to the operation with pressurized air, with some thrust advantage of the LG at high-speed tests, attributed to the volume increase of gas during phase change, in accordance with the thermodynamic power cycle analysis.
Reading about Gastronomy—An approach to Food Contents in New York City’s Newspapers
Authors: Fusté-Forné Francesc
Published: September 2020

Food and gastronomy are significant ingredients of everyday leisure and lifestyle practices. Food is part of culture and culture is part of the media. The current research analyzes the mediatization of food in legacy media. Drawing from a quantitative approach, the paper reviews food-based contents in New York City’s newspapers. In particular, AM New York, El Diario, Metro, The New York Times and The Wall Street Journal are studied over a period of 50 days. As a result, a total of 287 articles are analyzed. This research highlights the features of food and gastronomy contents and describes the differences and similarities between traditional newspapers and free dailies. Furthermore, the referent role of The New York Times in communicating food is confirmed.
Science-Based Strategies of Antiviral Coatings with Viricidal Properties for the COVID-19 Like Pandemics
Authors: Pemmada Rakesh , Zhu Xiaoxian , Dash Madhusmita , Zhou Yubin , Ramakrishna Seeram , Peng Xinsheng , Thomas Vinoy , Jain Sanjeev , Nanda Himansu Sekhar
Published: September 2020

The worldwide, extraordinary outbreak of coronavirus pandemic (i.e., COVID-19) and other emerging viral expansions have drawn particular interest to the design and development of novel antiviral, and viricidal, agents, with a broad-spectrum of antiviral activity. The current indispensable challenge lies in the development of universal virus repudiation systems that are reusable, and capable of inactivating pathogens, thus reducing risk of infection and transmission. In this review, science-based methods, mechanisms, and procedures, which are implemented in obtaining resultant antiviral coated substrates, used in the destruction of the strains of the different viruses, are reviewed. The constituent antiviral members are classified into a few broad groups, such as polymeric materials, metal ions/metal oxides, and functional nanomaterials, based on the type of materials used at the virus contamination sites. The action mode against enveloped viruses was depicted to vindicate the antiviral mechanism. We also disclose hypothesized strategies for development of a universal and reusable virus deactivation system against the emerging COVID-19. In the surge of the current, alarming scenario of SARS-CoV-2 infections, there is a great necessity for developing highly-innovative antiviral agents to work against the viruses. We hypothesize that some of the antiviral coatings discussed here could exert an inhibitive effect on COVID-19, indicated by the results that the coatings succeeded in obtaining against other enveloped viruses. Consequently, the coatings need to be tested and authenticated, to fabricate a wide range of coated antiviral products such as masks, gowns, surgical drapes, textiles, high-touch surfaces, and other personal protective equipment, aimed at extrication from the COVID-19 pandemic.
Comparative Research on Tensile Properties of Cement–Emulsified Asphalt–Standard Sand (CAS) Mortar and Cement–Emulsified Asphalt–Rubber Particle (CAR) Mortar
Authors: Li Chaoyuan , Liu Zanqun , Chen Juan , Yuan Qiang
Published: September 2020

The paper compared the tensile strength and elongation at break of cement–emulsified asphalt–standard sand (CAS) mortar and cement–emulsified asphalt–rubber particle (CAR) mortar. The tensile properties of CAS and CAR mortars were investigated. Microscopic analysis was carried out by Environmental Scanning Electron Microscopy and Energy Dispersive Spectrometer. The test results showed that the tensile strength of the CAR mortar at 7 days improved by about 9.09% higher than that of the CAS mortar, and further increased to 17.76% higher at 28 days The values of elongation at break of the CAR mortars at 3 days, 7 days, and 28 days increased by about 70% higher than those of the CAS mortars. Microscopic analysis showed that in the hardened CAS mortar, an obvious bubble accumulation layer with many pores appeared at the interfacial transition zone (ITZ). In the hardened CAR mortar, asphalt wrapped both cement hydration products and rubber particles and formed an integrated structure where a relatively dense and strong ITZ was formed as a result. This paper proves that the CAR system has superior tensile properties and has a promising future in waste rubber disposal.
Deformation Behavior of Al/Cu Clad Composite During Twist Channel Angular Pressing
Authors: Kocich Radim
Published: September 2020

The research and development of modern metallic materials goes hand in hand with increasing their lifetime via optimized deformation processing. The presented work deals with preparation of an Al/Cu clad composite with implemented reinforcing Cu wires by the method of twist channel angular pressing (TCAP). Single and double pass extrusion of the clad composite was simulated numerically and carried out experimentally. This work is unique as no such study has been presented so far. Detailed monitoring of the deformation behavior during both the passes was enabled by superimposed grids and sensors. Both the sets of results revealed that already the single pass imparted significant effective strain (higher than e.g., conventional equal channel angular pressing (ECAP)), especially to the Al matrix, and resulted in notable deformation strengthening of both the Al and Cu composite components, which was confirmed by the increased punch load and decreased plastic flow velocity (second pass compared to first pass). Processing via the second pass also resulted in homogenization of the imposed strain and residual stress across the composite cross-section. However, the investigated parameters featured slight variations in dependence on the monitored location across the cross-section.
Effects of a Novel Bioactive Glass Composition on Biological Properties of Human Dental Pulp Stem Cells
Authors: Di Tinco Rosanna , Sergi Rachele , Bertani Giulia , Pisciotta Alessandra , Bellucci Devis , Carnevale Gianluca , Cannillo Valeria , Bertoni Laura
Published: September 2020

Functional reconstruction of bone defects represents a clinical challenge in the regenerative medicine field, which targets tissue repair following traumatic injuries and disease-related bone deficiencies. In this regard, the optimal biomaterial should be safe, biocompatible and tailored in order to promote the activation of host progenitor cells towards bone repair. Bioactive glasses might be suitable biomaterials due to their composition being able to induce the host healing response and, eventually, anti-bacterial properties. In this study we investigated whether and how an innovative bioactive glass composition, called BGMS10, may affect cell adhesion, morphology, proliferation, immunomodulation and osteogenic differentiation of human dental pulp stem cells (hDPSCs). When cultured on BGMS10, hDPSCs maintained their proliferation rate and typical fibroblast-like morphology, showing the expression of stemness markers STRO-1 and c-Kit. Moreover, the expression of FasL, a key molecule in mediating immunomodulation effects of hDPSCs, was maintained. BGMS10 also proved to trigger osteogenic commitment of hDPSCs, as confirmed by the activation of bone-related transcription factors RUNX2 and Osx and the ongoing deposition of extracellular matrix supported by the expression of OPN and OCN. Our findings suggest that BGMS10 not only maintains the typical biological and immunomodulatory properties of hDPSCs but also favors the osteogenic commitment.
Propagation Speed of Dynamic Mode-I Cracks in Self-Compacting Steel Fiber-Reinforced Concrete
Authors: Pan Kaiming , Yu Rena C. , Zhang Xiaoxin , Ruiz Gonzalo , Wu Zhimin
Published: September 2020

The objective of this study is to measure the crack propagation speed in three types of self-compacting concrete reinforced with steel fibers loaded under four different loading rates. Central-notched prismatic beams with two types of fibers (13 mm and 30 mm in length), three fiber volume ratios, 0.51%, 0.77% and 1.23%, were fabricated. Four strain gages were glued on one side of the specimen notch to measure the crack propagation velocity, a fifth one at the notch tip to estimate the strain rates upon the initiation of a cohesive crack and the stress-free crack. A servo-hydraulic testing machine and a drop-weight impact device were employed to conduct three-point bending tests at four loading-point displacement rates, the former to perform tests at 2.2 μm/s, 22 mm/s and the latter for those at 1.77 m/s, 2.66 m/s, respectively. With lower fiber contents, smooth mode-I cracks were formed, the crack speed reached the order of 1 mm/s and 20 m/s. However, crack velocities up to 1417 m/s were obtained for the concrete with high content of fibers under impact loading. This value is fairly close to the theoretically predicted terminal crack velocity of 1600–1700 m/s. Numerical simulations based on cohesive theories of fracture and preliminary results based on the technique of Digital Image Correlation are also presented to complement those obtained from the strain gages. In addition, the toughness indices are calculated under all four loading rates. Strain hardening (softening) behavior accounting from the initiation of the first crack is observed for all three types of concrete at low (high) loading rates. Significant enhancement in the energy absorption capacity is observed with increased fiber content.
Temperature Dependence on Tensile Mechanical Properties of Sintered Silver Film
Authors: Wakamoto Keisuke , Mochizuki Yo , Otsuka Takukazu , Nakahara Ken , Namazu Takahiro
Published: September 2020

This paper investigates the influence of temperature on tensile mechanical properties of sintered silver (s-Ag) films with 8–10 μm in thickness for fundamental reliability design of semiconductor systems. The s-Ag film sintered under a pressure of 60 MPa possesses the porosity (p) around 5% determined from cross-sectional scanning electron microscope (SEM) images. The stress–strain (S-S) curves of s-Ag and pure silver (p-Ag) films are obtained using originally designed uniaxial tensile tester at temperatures from 25 °C to 150 °C. The S-S curves of p-Ag indicate ductile behavior irrespective of temperature, whereas those of s-Ag indicate brittle-ductile transition at 120 °C. Compared with p-Ag, s-Ag possesses low Young’s modulus (E) and high ultimate tensile strength (UTS) below 80 °C. The mechanism of brittle-ductile transition is discussed based on fracture surface observation results.
Investigation of Fracture Behavior and Mechanism in High-Speed Precise Shearing for Metal Bars with Prefabricated Fracture-Start Kerfs
Authors: Dong Yuanzhe , Ning Jinqiang , Dong Peng , Ren Yujian , Zhao Shengdun
Published: September 2020

A laser-assisted high-speed shearing (LAHSS) method has been proposed for metal bars, which prefabricates equally spaced fracture-start kerfs by Nd:Yag laser to make stress concentration, and applies a high-speed load to complete fracture separation. Comparative tests were conducted for Q235, 40Cr, and 304 steel bars, and the effects of fracture-start kerfs and axial clearance were investigated on the fracture section. Moreover, the fracture behavior was demonstrated by numerical simulation, and the micro-fracture mechanism was revealed by fractographic analysis. The numerical simulation results show that the material damage concentrates along with the kerf tips with peak equivalent plastic strain, and the corresponding stress triaxiality drops to almost zero at the kerf tip, which reveals that the material is subjected to pure shearing at kerf tip; the Max. loading force is reduced by 15.2%–29.6%, and the impact energy is decreased by 29.8%–46.9% for the three types of bar material. The experimental results showed that the fracture-start kerfs effectively inhibited the plastic deformation stage, and higher precision blanks were obtained in the LAHSS test: roundness error improved from 2.7%–10.9% to 1.1%–2.6%, Max. bending deflection decreased from 1.3–3.4 mm to 0.4–1.0 mm, and flatness error dropped from 0.9–3.3 mm to 0.3–0.7 mm. The fractographic analysis reveals that the crack initiation is related to alternative V-shape micro-notches at the laser-affected zone; the predominant fracture mechanism involves mode II microvoid coalescence at the main fracture plane; smaller and less elongated dimples were formed in 40Cr steels due to higher number density of grains and pinning effect of second-phase particles compared to Q235 and 304 steel bars.
Experimental Study on the Skidding Damage of a Cylindrical Roller Bearing
Authors: Zhang Qing , Luo Jun , Xie Xiang-yu , Xu Jin , Ye Zhen-huan
Published: September 2020

As large-scale rotating machines develop toward high rotating speed and high power–weight ratio, skidding damage has become one of the major initial failure modes of cylindrical roller bearings. Therefore, understanding the skidding damage law is an effective way to ensure the safety of machines supported by cylindrical roller bearings. To realize the skidding damage, a high-speed rolling bearing test rig that can simulate the actual operating conditions of aviation bearings was used in this paper, and the skidding damage dynamic behaviors of cylindrical roller bearings were investigated. In addition, to ensure the accuracy of the obtained skidding damage mechanism, the cylindrical roller bearing was carefully inspected by microscopic analysis when the skidding damage occurred. Out results show that instantaneous increases in friction torque, vibration acceleration, and temperature are clearly observed when the skidding damage occurs in the cylindrical roller bearing. Furthermore, under the conditions of inadequate lubrication and light load, the critical speed of skidding damage is rather low. The major wear mechanisms of skidding damage include oxidation wear, abrasive wear, and delamination wear. The white layers are found locally in the inner ring and rollers under the actions of friction heat and shear force.
Synthesis, Absolute Configuration, Antibacterial, and Antifungal Activities of Novel Benzofuryl β-Amino Alcohols
Authors: Tafelska-Kaczmarek Agnieszka , Kołodziejska Renata , Kwit Marcin , Stasiak Bartosz , Wypij Magdalena , Golińska Patrycja
Published: September 2020

A series of new benzofuryl α-azole ketones was synthesized and reduced by asymmetric transfer hydrogenation (ATH). Novel benzofuryl β-amino alcohols bearing an imidazolyl and triazolyl substituents were obtained with excellent enantioselectivity (96–99%). The absolute configuration (R) of the products was confirmed by means of electronic circular dichroism (ECD) spectroscopy supported by theoretical calculations. Selected benzofuryl α-azole ketones were also successfully asymmetrically bioreduced by fungi of Saccharomyces cerevisiae and Aureobasidium pullulans species. Racemic and chiral β-amino alcohols, as well as benzofuryl α-amino and α-bromo ketones were evaluated for their antibacterial and antifungal activities. From among the synthesized β-amino alcohols, the highest antimicrobial activity was found for (R)-1-(3,5-dimethylbenzofuran-2-yl)-2-(1H-imidazol-1-yl)ethan-1-ol against S. aureus ATCC 25923 (MIC = 64, MBC = 96 μg mL−1) and (R)-1-(3,5-dimethylbenzofuran-2-yl)-2-(1H-1,2,4-triazol-1-yl)ethan-1-ol against yeasts of M. furfur DSM 6170 (MIC = MBC = 64 μg mL−1). In turn, from among the tested ketones, 1-(benzofuran-2-yl)-2-bromoethanones (1–4) were found to be the most active against M. furfur DSM 6170 (MIC = MBC = 1.5 μg mL−1) (MIC—minimal inhibitory concentration, MBC—minimal biocidal concentration).
Unique Fixed-Point Results for β-Admissible Mapping under (β-ψˇ)-Contraction in Complete Dislocated Gd-Metric Space
Authors: Al-Mazrooei Abdullah , Shoaib Abdullah , Ahmad Jamshaid
Published: September 2020

This paper is designed to display some results which generalize the recent results that cannot be established from the corresponding results in other spaces and do not satisfy the remarks of Jleli et al. (Fixed Point Theor Appl. 210, 2012) and Samet et al. (Int. J. Anal. Article ID 917158, 2013). We obtain unique fixed-point for mapping satisfying β-ψˇ contraction only on a closed Gd ball in complete dislocated Gd-metric space. An example is also discussed to shed light on the main result.
How Are Medicinal Plants Useful When Added to Foods?
Authors: Nieto Gema
Published: September 2020

Consumers are concerned about the use of synthetic additives in foods and this has forced food processors to find ways to produce food products without the use of these additives [...]
Regulating Oral Biofilm from Cariogenic State to Non-Cariogenic State via Novel Combination of Bioactive Therapeutic Composite and Gene-Knockout
Authors: Chen Hong , Yang Yingming , Weir Michael D. , Dai Quan , Lei Lei , Homayounfar Negar , Oates Thomas W. , Yang Kai , Zhang Ke , Hu Tao , Xu Hockin H. K.
Published: September 2020

The objectives were to investigate a novel combination of gene-knockout with antimicrobial dimethylaminohexadecyl methacrylate (DMAHDM) composite in regulating oral biofilm from a cariogenic state toward a non-cariogenic state. A tri-species biofilm model included cariogenic Streptococcus mutans (S. mutans), and non-cariogenic Streptococcus sanguinis (S. sanguinis) and Streptococcus gordonii (S. gordonii). Biofilm colony-forming-units (CFUs), lactic acid and polysaccharide production were measured. TaqMan real-time-polymerase-chain reaction was used to determine the percentage of each species in biofilm. The rnc gene-knockout for S. mutans with DMAHDM composite reduced biofilm CFU by five logs, compared to control (p < 0.05). Using parent S. mutans, an overwhelming S. mutans percentage of 68.99% and 69.00% existed in biofilms on commercial composite and 0% DMAHDM composite, respectively. In sharp contrast, with a combination of S. mutans rnc knockout and DMAHDM composite, the cariogenic S. mutans percentage in biofilm was reduced to only 6.33%. Meanwhile, the non-cariogenic S. sanguinis + S. gordonii percentage was increased to 93.67%. Therefore, combining rnc-knockout with bioactive and therapeutic dental composite achieved the greatest reduction in S. mutans, and the greatest increase in non-cariogenic species, thereby yielding the least lactic acid-production. This novel method is promising to obtain wide applications to regulate biofilms and inhibit dental caries.
Effects of Ultrasonic-Assisted Extraction on the Yield and the Antioxidative Potential of Bergenia emeiensis Triterpenes
Authors: Luo Siyuan , Zeng Chen , Li Jiajia , Feng Shiling , Zhou Lijun , Chen Tao , Yuan Ming , Huang Yan , Yang Hongyu , Ding Chunbang
Published: September 2020

This study was the first designed to evaluate the extraction and antioxidant ability of triterpenes from Bergenia emeiensis rhizomes. The yield of triterpenes from B. emeiensis was mainly affected by the concentration of ethanol, followed by the extraction time, solvent to sample ratio, and the power of ultrasound. Thus, the response surface method was applied to investigate the interaction between the two factors and to optimize the extraction process. The optimal extraction conditions were 210 W, 75% ethanol, 40 min and 25 mL/g with a maximum yield of 229.37 ± 7.16 mg UAE/g. Moreover, the antioxidant ability of triterpenes from B. emeiensis (TBE) was evaluated by determining the scavenging capacity on free radicals and the protection on CHO cells and Caenorhabditis elegans against oxidative stress. The results showed the triterpenes could clear 2,2-Diphenyl-1-picryl-hydrazyl (DPPH) radicals well and had a strong reducing power. In addition, the survival of CHO cells was higher than that of the control group as a result of reducing the reactive oxygen species (ROS) level and promoting the activities of antioxidant enzymes. In addition, TBE could also enhance the survival of C. elegans under H2O2 conditions. Therefore, triterpenes from B. emeiensis could be developed into a beneficial potential for antioxidants.
Cichorins D–F: Three New Compounds from Cichorium intybus and Their Biological Effects
Authors: Khan Muhammad Farooq , Nasr Fahd A. , Noman Omar M. , Alyhya Nouf Abdulaziz , Ali Iftikhar , Saoud Mohamad , Rennert Robert , Dube Mthandazo , Hussain Wahid , Green Ivan R. , Basudan Omer Ahmed M. , Ullah Riaz , Anazi Shamsa Hilal , Hussain Hidayat
Published: September 2020

Cichorium intybus L., (chicory) is employed in various traditional medicines to treat a wide range of diseases and disorders. In the current investigation, two new naphthalane derivatives viz., cichorins D (1) and E (2), along with one new anthraquinone cichorin F (3), were isolated from Cichorium intybus. In addition, three previously reported compounds viz., β-sitosterol (4), β-sitosterol β-glucopyranoside (5), and stigmasterol (6) were also isolated from Cichorium intybus. Their structures were established via extensive spectroscopic data, including 1D (1H and 13C) and 2D NMR (COSY, HSQC and HMBC), and ESIMS. Cichorin E (2) has a weak cytotoxic effect on breast cancer cells (MDA-MB-468: IC50: 85.9 µM) and Ewing’s sarcoma cells (SK-N-MC: IC50: 71.1 µM); cichorin F (3) also illustrated weak cytotoxic effects on breast cancer cells (MDA-MB-468: IC50: 41.0 µM and MDA-MB-231: IC50: 45.6 µM), and SK-N-MC cells (IC50: 71.9 µM). Moreover compounds 1–3 did not show any promising anthelmintic effects.
The Effect of Moisture on the Adhesion Energy and Nanostructure of Asphalt-Aggregate Interface System Using Molecular Dynamics Simulation
Authors: Cui Wentian , Huang Wenke , Xiao Zhicheng , Xie Jiawen , Hu Bei , Cai Xu , Wu Kuanghuai
Published: September 2020

In this work, the influences of moisture intruded into the asphalt-aggregate interface have been investigated at the atomistic scale. The molecular interactions of asphalt with limestone and granite were studied using molecular dynamics (MD) simulations and the mineral surface components of limestone and granite were detected using the hyperspectral image technique. Relative concentration and radial distribution function (RDF) were employed for the characterization of asphalt component aggregations on aggregates surface. Adhesion work and debonding energy were also evaluated to investigate interface energy variations in asphalt-aggregate systems. MD results showed that the presence of interfacial moisture modified asphalt nanostructure and affected the aggregation state and distribution characteristics of asphalt components near aggregate surface. The study also demonstrated that the external moisture that intruded into the interface of the asphalt-aggregate system can decrease the concentration distribution of the asphalt components with powerful polarity on aggregate surface, reduce the adhesion works of the asphalt-aggregate interface, and decline the water damage resistance of asphalt mixture.
Small Physical Cross-Linker Facilitates Hyaluronan Hydrogels
Authors: Erikci Saliha , Mundinger Patricia , Boehm Heike
Published: September 2020

In this study, we demonstrate that small charged molecules (NH4+, GluA+, dHA+) can form physical cross-links between hyaluronan chains, facilitating polymerization reactions between synthetically introduced thiol groups (HA-DTPH). These hybrid hydrogels can be obtained under physiological conditions ideally suited for 3D cell culture systems. The type and concentration of a physical crosslinker can be adjusted to precisely tune mechanical properties as well as degradability of the desired hydrogel system. We analyze the influence of hydrogen bond formation, concentration and additional ionic interactions on the polymerization reaction of HA-DTPH hydrogels and characterize the resulting hydrogels in regard to mechanical and biocompatibility aspects.
The Reactions of H2O2 and GSNO with the Zinc Finger Motif of XPA. Not A Regulatory Mechanism, But No Synergy with Cadmium Toxicity
Authors: Witkiewicz-Kucharczyk Aleksandra , Goch Wojciech , Olędzki Jacek , Hartwig Andrea , Bal Wojciech
Published: September 2020

Tetrathiolate zinc fingers are potential targets of oxidative assault under cellular stress conditions. We used the synthetic 37-residue peptide representing the tetrathiolate zinc finger domain of the DNA repair protein XPA, acetyl-DYVICEECGKEFMSYLMNHFDLPTCDNCRDADDKHK-amide (XPAzf) as a working model to study the reaction of its Zn(II) complex (ZnXPAzf) with hydrogen peroxide and S-nitrosoglutathione (GSNO), as oxidative and nitrosative stress agents, respectively. We also used the Cd(II) substituted XPAzf (CdXPAzf) to assess the situation of cadmium assault, which is accompanied by oxidative stress. Using electrospray mass spectrometry (ESI-MS), HPLC, and UV-vis and circular dichroism spectroscopies we demonstrated that even very low levels of H2O2 and GSNO invariably cause irreversible thiol oxidation and concomitant Zn(II) release from ZnXPAzf. In contrast, CdXPAzf was more resistant to oxidation, demonstrating the absence of synergy between cadmium and oxidative stresses. Our results indicate that GSNO cannot act as a reversible modifier of XPA, and rather has a deleterious effect on DNA repair.
Sprouts and Needles of Norway Spruce (Picea abies (L.) Karst.) as Nordic Specialty—Consumer Acceptance, Stability of Nutrients, and Bioactivities during Storage
Authors: Jyske Tuula , Järvenpää Eila , Kunnas Susan , Sarjala Tytti , Raitanen Jan-Erik , Mäki Maarit , Pastell Helena , Korpinen Risto , Kaseva Janne , Tupasela Tuomo
Published: September 2020

Developing shoots, i.e., sprouts, and older needles of Norway spruce (Picea abies (L.) Karst.) have traditionally been used for medicinal purposes due to the high content of vitamins and antioxidants. Currently, sprouts are available as, for example, superfood and supplements. However, end-product quality and nutritive value may decline in the value-chain from raw material sourcing to processing and storage. We studied (1) impacts of different drying and extraction methods on nutritional composition and antioxidative properties of sprouts and needles, (2) differences between sprouts and needles in nutritional composition and microbiological quality, and (3) production scale quality of the sprouts. Additionally, (4) sprout powder was applied in products (ice-cream and sorbet) and consumer acceptance was evaluated. According to our results, older needles have higher content of dry matter, energy, and calcium, but lower microbial quality than sprouts. Sprouts showed a higher concentration of vitamin C, magnesium, potassium, and phosphorus than older needles. Freeze-drying was the best drying method preserving the quality of both sprouts and needles, e.g., vitamin C content. The antioxidative activity of the sprout extracts were lower than that of needles. Ethanol-water extraction resulted in a higher content of active compounds in the extract than water extraction. Sensory evaluation of food products revealed that on average, 76% of consumers considered sprout-containing products very good or good, and a creamy product was preferred over a water-based sorbet.
In Vitro Selection of an ATP-Binding TNA Aptamer
Authors: Zhang Li , Chaput John C.
Published: September 2020

Recent advances in polymerase engineering have made it possible to isolate aptamers from libraries of synthetic genetic polymers (XNAs) with backbone structures that are distinct from those found in nature. However, nearly all of the XNA aptamers produced thus far have been generated against protein targets, raising significant questions about the ability of XNA aptamers to recognize small molecule targets. Here, we report the evolution of an ATP-binding aptamer composed entirely of α-L-threose nucleic acid (TNA). A chemically synthesized version of the best aptamer sequence shows high affinity to ATP and strong specificity against other naturally occurring ribonucleotide triphosphates. Unlike its DNA and RNA counterparts that are susceptible to nuclease digestion, the ATP-binding TNA aptamer exhibits high biological stability against hydrolytic enzymes that rapidly degrade DNA and RNA. Based on these findings, we suggest that TNA aptamers could find widespread use as molecular recognition elements in diagnostic and therapeutic applications that require high biological stability.
Study on Synergistic Corrosion Inhibition Effect between Calcium Lignosulfonate (CLS) and Inorganic Inhibitors on Q235 Carbon Steel in Alkaline Environment with Cl−
Authors: Lin Bing , Tang Junlei , Wang Yingying , Wang Hu , Zuo Yu
Published: September 2020

The synergistic corrosion inhibition effect between calcium lignosulfonate (CLS) and three kinds of inorganic inhibitors (Na2MoO4, Na2SnO3, and NaWO4) with various molar ratios on Q235 carbon steel in alkaline solution (pH 11.5) with 0.02 mol/L NaCl was investigated by cyclic potentiodynamic polarization, electrochemical impedance spectroscopy, linear polarization, scanning electron microscopy, and X-ray photoelectron spectroscopy. Molybdate and stannate in hybrid inhibitor could promote the passivation of steel and form a complex film, which could suppress the corrosion effectively. Moreover, the insoluble metal oxides in the complex film formed by three kinds of inorganic inhibitor could help the adsorption of CLS onto the steel surface. The CLS molecules could adsorb onto the steel surface and metal oxides to form an adsorption film to protect the steel from corrosion. A three-layer protection film formed by a hybrid inhibitor, including passivation film, deposition film, and adsorption film, would effectively inhibit the corrosion reactions on the steel surface. The CLS compound with molybdate with the ratio of 2:3 shows the best inhibition effect on both general corrosion and localized corrosion.
Flavonoids as Phytoestrogenic Components of Hops and Beer
Authors: Tronina Tomasz , Popłoński Jarosław , Bartmańska Agnieszka
Published: September 2020

The value of hops (Humulus lupulus L.) in beer production has been undisputed for centuries. Hops is rich in humulones and lupulones which gives the characteristic aroma and bitter taste, and preserves this golden drink against growing bacteria and molds. Besides α- and β-acids, the lupulin glands of hop cones excrete prenylated flavonoids, which exhibit a broad spectrum of biological activities and therefore has therapeutic potential in humans. Recently, interest in hops was raised due to hop prenylated flavanones which show extraordinary estrogen activities. The strongest known phytoestrogen so far is 8-prenylnaringenin (8-PN), which along with 6-prenylanaringenin (6-PN), 6,8-diprenylnaringenin (6,8-DPN) and 8-geranylnaringenin (8-GN) are fundamental for the potent estrogen activity of hops. This review provides insight into the unusual hop phytoestrogens and shows numerous health benefits associated with their wide spectrum of biological activities including estrogenic, anticancer, neuropreventive, antinflamatory, and antimicrobial properties, which were intensively studied, and potential applications of these compounds such as, as an alternative to hormone replacement therapy (HRT).
Sinensol-C Isolated from Spiranthes sinensis Inhibits Adipogenesis in 3T3-L1 Cells through the Regulation of Adipogenic Transcription Factors and AMPK Activation
Authors: Shie Pei-Hsin , Yang Chung-Ping , Huang Guan-Jhong , Wang Sheng-Yang , Kuo Yueh-Hsiung
Published: September 2020

Obesity is an abnormal medical condition caused by accumulation of body fat that presents negative health impacts. Adipocyte hyperplasia, also known as adipogenesis, is one of the major manifestations of obesity. In the present study, we isolated six phenanthrene derivatives (compounds 1–6) from the ethyl acetate fraction of Spiranthes sinensis and investigated their anti-adipogenic activity. We found that among the six phenanthrene derivatives, compound 6 (sinensol-C) exhibited strong inhibitory activity against intracellular lipid accumulation in 3T3-L1 adipocytes, with an IC50 value of 12.67 μM. Sinensol-C remarkably suppressed the accumulation of lipid droplets and adipogenesis, via down-regulation of adipogenic transcription factors, including peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein α (C/EBPα), sterol regulatory element binding protein-1 (SREBP-1c), fatty acid synthase (FAS), and fatty acid binding protein 4 (FABP4), during adipocyte differentiation in 3T3-L1 cells. In addition, treatment with sinensol-C significantly increased the adenosine monophosphate-activated protein kinase (AMPK) activity in 3T3-L1 cells. Taken together, these data strongly suggest that sinensol-C regulates adiogenesis via down-regulation of adipogenic transcription factors and up-regulation of AMPK. Furthermore, this is the first study that demonstrates that sinensol-C has the capacity to modulate adipogenesis.
Optimization of High-Pressure Extraction Process of Antioxidant Compounds from Feteasca regala Leaves Using Response Surface Methodology
Authors: Becze Anca , Babalau-Fuss Vanda Liliana , Varaticeanu Cerasel , Roman Cecilia
Published: September 2020

Circular economy principles are based on the use of by-products from one operation as the raw materials in another. The aim of this work is to obtain extracts with high antioxidant capacity and resveratrol content for the superior capitalization of the biomass of Feteasca regala leaves obtained during vineyard horticultural operations in spring. In order to obtain a high-quality extract at an industrial level, an optimal extraction process is needed. Central composite design (CCD) was used for the experiment design, which contained three independent variables: the ratio of extraction solvent to solid matter, temperature (°C) and time (minutes). The evaluation of extracts was done by measuring the total antioxidant capacity of the extracts using photo-chemiluminescent techniques, and the resveratrol content using liquid chromatography. Process optimization was done using response surface methodology (RSM). Minitab software version 17.0 was used for the design of experiments and data analysis. Regression analysis showed that the model predicts 87.5% of the variation for resveratrol and 96% for total antioxidant capacity (TAC). The temperature had the biggest influence on the extraction yield. The optimal operational conditions for the extraction method applied had the following conditions: ratio e/m 2.92; 43.23 °C and 55.4 min. A maximum value of 34,623 µg ascorbic acid equivalent (AAE) /mL total antioxidant capacity and 182.4 µg/mL resveratrol content were obtained when the optimal extraction parameters where used. The values obtained in experiments proved that by using RSM an accurate model can be obtained for extraction of Feteasca regala leaves.
Heparan Sulfate Proteoglycans Biosynthesis and Post Synthesis Mechanisms Combine Few Enzymes and Few Core Proteins to Generate Extensive Structural and Functional Diversity
Authors: Annaval Thibault , Wild Rebekka , Crétinon Yoann , Sadir Rabia , Vivès Romain R. , Lortat-Jacob Hugues
Published: September 2020

Glycosylation is a common and widespread post-translational modification that affects a large majority of proteins. Of these, a small minority, about 20, are specifically modified by the addition of heparan sulfate, a linear polysaccharide from the glycosaminoglycan family. The resulting molecules, heparan sulfate proteoglycans, nevertheless play a fundamental role in most biological functions by interacting with a myriad of proteins. This large functional repertoire stems from the ubiquitous presence of these molecules within the tissue and a tremendous structural variety of the heparan sulfate chains, generated through both biosynthesis and post synthesis mechanisms. The present review focusses on how proteoglycans are “gagosylated” and acquire structural complexity through the concerted action of Golgi-localized biosynthesis enzymes and extracellular modifying enzymes. It examines, in particular, the possibility that these enzymes form complexes of different modes of organization, leading to the synthesis of various oligosaccharide sequences.
Designing for Temporal Harmony: Exploring the Well-Being Concept for Designing the Temporal Dimension of User Experience
Authors: Hermosa Perrino Cristina , Burmester Michael
Published: September 2020

User Experience (UX) is characterized by its temporal dimension, dynamic nature, and variability. Although descriptive models about the temporal dimension and related aspects exist, an understanding of the design possibilities and a design approach that ensures the design of the temporal dimension promoting a positive UX and well-being are still lacking. This paper addresses this research gap and builds on Zimbardo and Boyd’s Time Perspective Theory (TPT). TPT presents five time perspectives (TPs)—Past-Negative, Past-Positive, Present-Fatalistic, Present-Hedonistic, and Future—to reveal that people have individual attitudes toward time that influence their thoughts, actions, and feelings. Studies conclude that a balance between the positive TPs (Past-Positive, Present-Hedonistic, and Future), i.e., temporal harmony, contributes to long-term well-being. We present our design framework and approach “designing for temporal harmony,” which incorporates the theory into the practice to highlight the temporal design possibilities and to offer guidance for designers. We applied the design framework and approach to a case study, developed an app concept, and evaluated it with users. The results demonstrate that it is possible to systematically develop temporal UX concepts that evoke positive anticipations, experiences, and retrospections, and that these promote a positive UX as well as contribute to users’ long-term well-being.
Association of Dietary Inflammatory Index with Serum IL-6, IL-10, and CRP Concentration during Pregnancy
Authors: Pieczyńska Joanna , Płaczkowska Sylwia , Pawlik-Sobecka Lilla , Kokot Izabela , Sozański Rafał , Grajeta Halina
Published: September 2020

Background: The mother’s diet has a direct impact on fetal development and pregnancy, and can also be important in the course of the body’s inflammatory response. An anti-inflammatory diet can be a promising way to counter an excessive inflammatory response in pregnancy. Objective: The aim of the study was to examine the association between the dietary inflammatory index (DII) and the pregnant women’s serum interleukin 6 (IL-6) and 10 (IL-10) and C-reactive protein (CRP) concentration in the course of normal and complicated pregnancy. Research Methods and Procedures: The study included 45 Polish pregnant women recruited to the study. The DII, a literature-based dietary index to assess the inflammatory properties of diet, was estimated based on a seven-day 24-h recall and an food frequency questionnaire (FFQ) in each trimester of pregnancy. At the same time as the nutritional interviews, blood samples were collected for the determination of IL-6, IL-10, and CRP concentrations. The studied group was divided into subgroups with normal and complicated pregnancy and depending on the DII median. Results: With the development of pregnancy, the DII score slightly decreased in subsequent trimesters: −1.78 in the first trimester, −2.43 in the second trimester, and −2.71 in the third trimester (p = 0.092). Independent of the trimester of pregnancy and the occurrence of pregnancy complications, the DII score did not affect the differences in the serum concentrations of IL-6, IL-10, and CRP, with the exception of CRP level in the second trimester in women with complicated pregnancy (subgroup with DII < median had a lower CRP level than subgroup with DII > median). In the first and third trimesters, there was a weak but significant positive correlation between the DII score and CRP concentration. During the second trimester, in the group with normal pregnancy and DII below the median, a significant negative correlation between the DII score and the serum IL-6 and IL-10 concentration was noted as well as in the third trimester for IL-6. Conclusion: The anti-inflammatory potential of a pregnant woman’s diet increases slightly with pregnancy development; however, its value has no permanent significant association with the level of CRP, IL-6, and IL-10.
Vitamin D and Autoimmune Thyroid Disease—Cause, Consequence, or a Vicious Cycle?
Authors: Vieira Inês Henriques , Rodrigues Dírcea , Paiva Isabel
Published: September 2020

Vitamin D is a steroid hormone traditionally connected to phosphocalcium metabolism. The discovery of pleiotropic expression of its receptor and of the enzymes involved in its metabolism has led to the exploration of the other roles of this vitamin. The influence of vitamin D on autoimmune disease—namely, on autoimmune thyroid disease—has been widely studied. Most of the existing data support a relationship between vitamin D deficiency and a greater tendency for development and/or higher titers of antibodies linked to Hashimoto’s thyroiditis, Graves’ disease, and/or postpartum thyroiditis. However, there have also been some reports contradicting such relationships, thus making it difficult to establish a unanimous conclusion. Even if the existence of an association between vitamin D and autoimmune thyroid disease is assumed, it is still unclear whether it reflects a pathological mechanism, a causal relationship, or a consequence of the autoimmune process. The relationship between vitamin D’s polymorphisms and this group of diseases has also been the subject of study, often with divergent results. This text presents a review of the recent literature on the relationship between vitamin D and autoimmune thyroid disease, providing an analysis of the likely involved mechanisms. Our thesis is that, due to its immunoregulatory role, vitamin D plays a minor role in conjunction with myriad other factors. In some cases, a vicious cycle is generated, thus contributing to the deficiency and aggravating the autoimmune process.