bluuebunny/crossref_metadata_2025 huggingface dataset:
Wine Grape (Vitis vinifera) Response to Fall Exposure of Simulated Drift from Selected Herbicides
Authors: Bhatti Muhammad A. , Al-Khatib Kassim , Parker Robert
Published: March 2018

‘Lemberger’ wine grape response to fall application of selected herbicides applied at simulated drift rates was studied in 1992 and 1993. Chlorsulfuron, thifensulfuron, 2,4-D, glyphosate, bromoxynil, and 2,4-D plus glyphosate were applied at 1/100, 1/33, 1/10, and 1/3 of a selected maximum rate for use in wheat or fallow. All herbicides, except bromoxynil and thifensufluron, caused symptoms on grapevines at the highest rate during the spring following fall application. The most severe symptoms were caused by 2,4-D and 2,4-D plus glyphosate, whereas the least symptoms were caused by chlorsulfuron and glyphosate. The severity of symptoms increased and shoot growth, leaf area, internode length, and dry cane weight decreased as the rates of 2,4-D and 2,4-D plus glyphosate increased. Chlorsulfuron and glyphosate reduced the growth of grapevines only when applied at the highest rate during the fall. The data show that exposure of wine grapes to 2,4-D or 2,4-D plus glyphosate during the fall can adversely affect the growth of grapevines the following spring.
Weed Invasion in Established Alfalfa (Medicago sativa) Seeded with Perennial Forage Grasses
Authors: Spandl Eric , Kells James J. , Hesterman Oran B.
Published: March 2018

Component yields and forage quality were evaluated in established stands of alfalfa seeded alone and in binary mixtures with smooth bromegrass, orchardgrass, or timothy. Forage yields and quality were determined at three annual harvests in the third and fourth years after seeding. Weed yields in alfalfa seeded alone were significantly greater than those in the alfalfa–orchardgrass mixture. Average weed content was 24, 17, 2, and 15% for the alfalfa seeded alone, alfalfa–bromegrass, alfalfa–orchardgrass, and alfalfa–timothy, respectively. Seeding alfalfa with orchardgrass increased first harvest and annual forage yields while reducing alfalfa yield. Yields of orchardgrass often exceeded those of bromegrass or timothy. In the first harvest each year, crude protein was reduced and acid detergent fiber and neutral detergent fiber increased by including orchardgrass. Neutral detergent fiber was greater in alfalfa–orchardgrass than in the alfalfa seeded alone at most harvests. There were no consistent differences among the alfalfa seeded alone and the other mixtures in yield or quality.
SAN 582, Alachlor, and Metolachlor Control Triazine-Resistant (TR) Smooth Pigweed (Amaranthus hybridus) in No-Till Corn (Zea mays)
Authors: Foy Chester L. , Witt Harold L.
Published: March 2018

SAN 582 at 1.1, 1.2, 1.3, 1.5, and 1.6 kg ai/ha; alachlor at 1.7, 2.2, and 2.8 kg/ha; and metolachlor at 1.7, 2.2, and 2.8 kg/ha were applied preemergence without and with atrazine at 1.7 kg/ha in no-till corn at two locations near Blacksburg, VA, in 1993 and 1994. SAN 582, alachlor, and metolachlor controlled TR-smooth pigweed when applied without or with atrazine, and with only a few exceptions, control was greater than 60%. The highest rate of individual herbicides was more effective than the lowest rate in most cases. Early control of TR-smooth pigweed was similar from all three herbicides, especially at higher rates, but control later in the season tended to be best with alachlor. As expected, atrazine applied alone did not control TR-smooth pigweed, and crop yields were increased with herbicide treatments compared to no treatment.
Sicklepod (Senna obtusifolia) Control in Soybean (Glycine max) with Imazaquin and Metribuzin Combinations
Authors: Etheridge Robert E. , Murdock Edward C. , Stapleton Gregory S. , Toler Joe E.
Published: March 2018

Field studies were conducted in 1993 and 1994 to evaluate sicklepod control with combinations of imazaquin + metribuzin at lower than normal use rates. Soybean was seeded in late-May to early-June each year and imazaquin and metribuzin were applied PPI alone at 0.75 and 1x and 0.5, 0.75, and 1x their registered rates, respectively, and in factorial combinations of their 0.5 and 0.75x rates. The registered (1x) rates for imazaquin and metribuzin on this soil type are 0.14 and 0.43 kg/ha, respectively. Several standard sequential treatments and flumetsulam + trifluralin PPI at 0.06 + 0.70 and 0.07 + 0.95 kg/ha were included for comparison. Sicklepod control and soybean seed yield responses differed between 1993 and 1994. In 1993, the combinations of imazaquin + metribuzin averaged 90% control 6 wk after planting (WAP) and soybean seed yield increased 75% compared to the untreated check. Imazaquin + metribuzin at their respective 0.5x rate was as effective as any treatment evaluated. In 1994, sicklepod control was generally lower with all treatments. Soybean seed yield was reduced due to sicklepod interference with soil-applied treatments alone. However, imazaquin + metribuzin at their respective 0.75x rate provided sicklepod control levels and reductions in weed biomass similar to those observed with flumetsulam + trifluralin at 0.07 + 0.95 kg/ha and the sequential treatments, and increased soybean seed yield 19% compared to the untreated check.
Ivyleaf Morningglory (Ipomoea hederacea) Interference with Cotton (Gossypium hirsutum)
Authors: Rogers Joy B. , Murray Don S. , Verhalen Laval M. , Claypool P. L.
Published: March 2018

Full-season interference and critical duration of early-season ivyleaf morningglory interference with cotton were measured in experiments at two Oklahoma locations. Densities ranging from 0 to 64 weeds/10 m of row were evaluated for their effects on cotton lint yield, fiber quality, and harvest efficiency. Analyses using linear-linear regression proved superior to linear, curvilinear, and linear-plateau models. Lint yield reductions of 36.9 kg/ha (or 5.9%) were recorded at Perkins for each weed/10 m up to 8.7. At densities greater than 8.7/10 m, yield was reduced an additional 3.0 kg/ha (0.5%) for each additional weed. At Chickasha, lint yield was reduced 29.7 kg/ha (or 3.9%) for each weed/10 m up to 9.0 with an additional lint loss of 3.6 kg/ha (0.7%) for each weed above the density of 9.0/10 m. Yield losses were over 80% before fiber length was also reduced. Mechanical harvest was not possible at densities above 8 weeds/10 m at Chickasha and above 16 weeds/10 m at Perkins. In critical duration experiments conducted at Perkins, lint yield was reduced 52.9 kg/ha (or 11.2%) for each week weed removal was delayed up to 9.5 wk. An additional 1.0 kg/ha (0.2%) was lost for each week of interference after 9.5 wk. At Chickasha, lint yield was reduced 49.0 kg/ha (or 7.8%) for each week of interference up to 11.0 wk with an additional loss of 1.2 kg/ha (0.2%) for each week thereafter.
The Effect of Piperonyl Butoxide and Adjuvants on Sulfonylurea Herbicide Activity
Authors: Kwon Chae Soon , Penner Donald
Published: March 2018

Greenhouse studies showed that the mixed function oxidase inhibitor, piperonyl butoxide (PBO), tank-mixed with the sulfonylurea herbicides, nicosulfuron, primisulfuron, and thifensulfuron, in the absence of effective adjuvants enhanced herbicide activity on both broadleaf and grass weed species. Effective adjuvants for nicosulfuron were K-3000 for common lambsquarters, Sylgard® 309 Surfactant for velvetleaf, K-2000 for barnyardgrass, and K-2000, K-3000, and Scoil® methylated seed oil for giant foxtail control. K-3000 and Sylgard 309 enhanced velvetleaf control with primisulfuron and thifensulfuron. The 28% urea and ammonium nitrate (UAN) was more effective as an adjuvant with thifensulfuron for velvetleaf than for common lambsquarters control. The enhancement of sulfonylurea herbicide activity with PBO was most apparent when other adjuvants were least effective.
Duration of Control from Preemergence Herbicides for Use in Nonburned Grass Seed Crops
Authors: Mueller-Warrant George W.
Published: March 2018

Legislatively mandated reductions in open field burning have increased grass seed growers reliance on herbicides to control seedling grasses in established stands of perennial species. In nonburned fields, a dense flush of seedling weeds and volunteer crop emerges with the onset of fall rains, and additional germination of some species occurs throughout the fall, winter, and spring. Because rainfall patterns in western Oregon are erratic during late summer and early fall, the choice of when to apply preemergence herbicides is difficult. Herbicides applied too early may dissipate before the rains arrive, whereas herbicides applied too late may not prevent germination and seedling establishment. Pendimethalin was least affected by a 3- to 4-wk exposure on dry soil waiting for rain, followed in order of increasing sensitivity by oxyfluorfen, metolachlor, and trifluralin. Most treatments controlled California brome less effectively than perennial ryegrass. Duration of 85% or greater control of subsequently oversown perennial ryegrass averaged 69 d for 2.2 kg/ha pendimethalin, 11 d for 0.42 kg/ha oxyfluorfen, and 4 d for 2.2 kg/ha metolachlor, and duration of 50% or greater control averaged 98, 33, and 18 d for the same treatments. Doubling of herbicide rates doubled the duration of control in 15 out of 27 cases but increased the duration only an average of 75% in the other 12 cases. Average time to doubling of weed populations over those occurring when herbicides and weed seeds were applied on the same day was 12, 10, and 11 d after application for pendimethalin, oxyfluorfen, and metolachlor, respectively. The similarity of these periods suggests that the major difference in performance of these herbicides with time was how close to complete control they had initially achieved. Pendimethalin was the superior preemergence herbicide for controlling California brome and perennial ryegrass in these established stands, but oxyfluorfen and metolachlor also were useful if applied near germination.
Response of Johnsongrass (Sorghum halepense) and Imidazolinone-Resistant Corn (Zea mays) to AC 263,222
Authors: Wilcut John W. , Richburg John S. , Walls F. Robert
Published: March 2018

Field studies were conducted in 1992 and 1993 to evaluate AC 263,222 applied postemergence (POST) alone and as a mixture with atrazine or bentazon for weed control in imidazolinone-resistant corn. Nicosulfuron alone and nicosulfuron plus atrazine were also evaluated. Herbicide treatments were applied following surface-banded applications of two insecticides, carbofuran or terbufos at planting. Crop sensitivity to POST herbicides, corn yield, and weed control was not affected by insecticide treatments. AC 263,222 at 36 and 72 g ai/ha controlled rhizomatous johnsongrass 88 and 99%, respectively, which was equivalent to nicosulfuron applied alone or with atrazine. AC 263,222 at 72 g/ha controlled large crabgrass 99% and redroot pigweed 100%, and this level of control exceeded that obtained with nicosulfuron alone. AC 263,222 at 72 g/ha controlled sicklepod and morningglory species 99 and 98%, respectively. Nicosulfuron alone or with atrazine controlled these two species less than AC 263,222 at 72 g/ha. Addition of bentazon or atrazine to AC 263,222 did not improve control of any species compared with the higher rate of AC 263,222 at 72 g/ha applied alone. Corn yield increased over the untreated control when POST herbicide(s) were applied, but there were no differences in yield among herbicide treatments.
Weed Management in Peanut (Arachis hypogaea) with Flumioxazin Preemergence
Authors: Askew Shawn D. , Wilcut John W. , Cranmer John R.
Published: March 2018

Flumioxazin plus metolachlor mixtures preemergence (PRE) were evaluated with or without postemergence (POST) herbicides for weed control and peanut (Arachis hypogaea) response in three North Carolina studies. Metolachlor PRE at 2.24 kg ai/ha controlled goosegrass (Eleusine indica) and yellow nutsedge (Cyperus esculentus) 93 and 80%, respectively, and control was not improved with flumioxazin or norflurazon. Metolachlor plus flumioxazin PRE at 0.07 or 0.11 kg ai/ha controlled common lambsquarters (Chenopodium album); entireleaf (Ipomoea hederaceavar.integriuscula), ivyleaf (I. hederacea), and pitted morningglory (I. lacunosa); and prickly sida (Sida spinosa) better than metolachlor plus norflurazon PRE at 1.34 kg ai/ha. Morningglories (Ipomoeaspp.) were controlled 77 and 86% with flumioxazin PRE at 0.07 and 0.11 kg/ha, respectively, and control was increased to nearly 100% with acifluorfen plus 2,4-DB or lactofen plus 2,4-DB POST. Peanut injury by flumioxazin and norflurazon was observed at one location in 1997; however, yields were not reduced. Peanut treated with metolachlor plus flumioxazin PRE at either rate yielded at least 3,750 kg/ha compared to 3,120 kg/ha with metolachlor plus norflurazon PRE or 1,320 kg/ha with metolachlor PRE.
Symposium on Managing Herbicide Resistant Weeds: Proceedings of a Symposium Sponsored by the North Central Weed Science Society and the North American Herbicide Resistance Working Group, December 10, 1998, St. Paul, MN
Authors: Retzinger E. James
Published: March 2018

Resistance of weeds to herbicides that were initially controlled by that herbicide has increased in all major crops and forages. An internet web site, http//weedscience.com, logs all confirmed resistant weed species and their location by country. Management of these species and the cost of those management techniques was the focus of this symposium. The group of scientists assembled for this symposium were chosen to discuss the systems currently utilized in the management of species that are resistant to a single or to multiple groups of herbicides. It is hoped that information presented on the cost of managing these resistant species will further encourage the judicious use of herbicides, the implementation of agronomic practices, and crop rotations to delay the development of resistance to other areas.
The Impact of Herbicide-Resistant Weeds on Kansas Agriculture
Authors: Peterson Dallas E.
Published: March 2018

Herbicides are important components of weed management programs for most Kansas farmers. Monocropping systems and repeated use of the same or similar herbicides in some areas of the state have resulted in the development of herbicide-resistant weeds. The development of herbicide-resistant weed populations can have an immediate and a long-term effect on the cost, implementation, and effectiveness of weed control programs. In Kansas, resistance to triazine herbicides has been confirmed in kochia (Kochia scoparia), redroot pigweed, common waterhemp (Amaranthus rudis), Palmer amaranth (Amaranthus palmeri), and downy brome (Bromus tectorum) populations, and resistance to acetolactate synthase (ALS)-inhibiting herbicides has been confirmed in kochia, Russian thistle (Salsola kali), common waterhemp, Palmer amaranth, common cocklebur (Xanthium strumarium), shattercane (Sorghum bicolor), and common sunflower (Helianthus annum). The frequency and distribution of herbicide resistance varies among species. Producers who experience herbicide resistance problems adjust their weed control program accordingly. Producers that have not encountered an herbicide resistance problem tend to continue with a successful herbicide program until it fails. The recommended management strategies for herbicide-resistant weed populations include an integrated system of crop rotation, rotation of herbicide modes of action, tank-mixes of herbicides with different modes of action, and cultivation. The greatest direct cost to the producer occurs during the first year of poor weed control. The first response to an herbicide failure often is to reapply the same herbicide that has worked well previously. By the time the producer realizes that the treatment is not going to work, it usually is too late for any other remedial action. Consequently, the farmer experiences reduced crop production from weed competition, high herbicide costs, and a tremendous increase in the seed bank. The increase in seed bank may cost the farmer the most in the long run because the increased weed pressure often requires an intensified control program for several years.
Counter-monuments and the Perdurance of Place
Authors: Watts Christopher M.
Published: March 2018

In this paper, I critically examine contemporary commemorative forms and practices known as ‘counter-monuments’ from an archaeological standpoint, in the process interrogating the conceptual underpinnings of the monument taxon as it is currently understood. Drawing on Tim Ingold's notion of perdurance, and through an exploration of counter-monumental concerns with form, siting and proxemics, I argue that memorialization can be seen as relationally emergent in the experiences of particular places. This claim is advanced through a discussion of the Cedar Creek Earthworks, a Woodland Period (c.1–1550ad) enclosure near Windsor, Canada, whose status as a monument can be understood, not as an ostentatious appeal to past events, but as a magnet for drawing out and assembling human and non-human relations in place.
Justice at the Workplace: A Review
Authors: VIRTANEN MARIANNA , ELOVAINIO MARKO
Published: March 2018

Modern work life is characterized by constant change, reorganizations, and requirements of efficiency, which make the distribution of resources and obligations, as well as justice in decisionmaking, highly important. In the work life context, it is a question not only of distributing resources and obligations, but also of the procedures and rules that guide the decisionmaking in the organization. Studies of these rules and procedures have provided the basis for a new line of research that evaluates leadership and social relationships in working communities; that is, distributive, procedural, and relational justice. This review follows the development of research on organizational justice from its origins in early social and motivational psychological theories to its establishment as a major line of research in modern work and organizational psychology. The adverse consequences of injustice include poor team climate, reduced productivity and well-being, and work-related illnesses.
Early childhood onset of high-grade atrioventricular block in Hunter syndrome
Authors: Chlebowski Meghan M. , Heese Bryce A. , Malloy-Walton Lindsey E.
Published: March 2018

Cardiac involvement has been reported in various mucopolysaccharidoses syndromes. Cardiac valve pathology is the most prominent cardiac manifestation of patients with these syndromes. To date, there have been no reports of early childhood onset of high-grade atrioventricular block in patients with Hunter syndrome. We present a case of a 3-year-old boy with Hunter syndrome who was found to have various degrees of atrioventricular block. This case highlights the importance of early routine cardiac screening for conduction abnormalities and close follow-up in patients with mucopolysaccharidoses syndromes.
Humanities Encounters Science: Confronting the Challenge of Post-humanism
Authors: Wang Ning
Published: March 2018

Post-humanism has recently come to China and challenges traditional humanism and the humanities. The author first offers a reflection on the evolution of humanism in modern Chinese intellectual history. To the author, we are now in a ‘post-theoretical era’, in which the function of theory is no longer so powerful and ubiquitous as it used to be. It is then argued that the rise of the ‘post-humanist’ trend in the West during the past decades indicates that, in the present era, humankind is only one of many species on earth, whose existence and development, to a large extent, depend on natural law. At the same time, post-humanism tells us that humankind may no longer be able to control some of its own creations. The author concludes by calling for digital humanities to bridge the gap between science and humanities and to establish a new relationship between the two.
Inverse association of calcium intake with abdominal adiposity and C-reactive protein in Brazilian children
Authors: Suhett Lara Gomes , Silveira Brenda Kelly Souza , Filgueiras Mariana De Santis , Peluzio Maria do Carmo Gouveia , Hermsdorff Helen Hermana Miranda , Novaes Juliana Farias de
Published: March 2018

Objective

To evaluate Ca intake and its association with cardiometabolic risk factors during childhood.

Design

A cross-sectional study with a representative sample. Food consumption was assessed through three 24 h dietary recalls. Anthropometry, body composition and biochemical measurements were also conducted.

Setting

Viçosa, Minas Gerais, Brazil.

Subjects

Children between 8 and 9 years old (n350) enrolled in public and private schools in the urban area of the municipality of Viçosa.

Results

Almost all children had inadequate intake of Ca (97·4 %), especially those with low income, non-white and who studied in public schools. Foods that contributed most to Ca intake were ‘milk’ and ‘cheeses and yoghurts’ (R2=0·66 and 0·13, respectively), and intake of ‘milk’ was correlated with ‘chocolate milk powder’ intake (r=0·538,P<0·01). Children with lower Ca intake had a higher prevalence of increased C-reactive protein (prevalence ratio=2·93; 95 % CI 1·21, 7·07), increased waist circumference (prevalence ratio=2·86; 95 % CI 1·01, 8·13) and a lower prevalence of high LDL cholesterol (prevalence ratio=0·64; 95 % CI 0·41, 0·99).

Conclusions

Lower Ca intake was associated with excess abdominal adiposity and subclinical inflammation in Brazilian children. Monitoring of adequate Ca intake is important, especially in poorer communities.
The global nutrition transition: trends, disease burdens and policy interventions
Authors: Ronto Rimante , Wu Jason HY , Singh Gitanjali M
Published: March 2018

Non-communicable diseases (NCD) have increased dramatically in developed and developing countries. Unhealthy diet is one of the major factors contributing to NCD development. Recent evidence has identified deterioration in aspects of dietary quality across many world regions, including low- and middle-income countries (LMIC). Most burdens of disease attributable to poor diet can be prevented or delayed as they occur prematurely. Therefore, it is important to identify and target unhealthy dietary behaviours in order to have the greatest impact. National dietary-related programmes have traditionally focused on micronutrient deficiency and food security and failed to acknowledge unhealthy dietary intakes as a risk factor that contributes to the development of NCD. Inadequate intakes of healthy foods and nutrients and excess intakes of unhealthy ones are commonly observed across the world, and efforts to reduce the double burden of micronutrient deficiency and unhealthy diets should be a particular focus for LMIC. Interventions and policies targeting whole populations are likely to be the most effective and sustainable, and should be prioritized. Population-based approaches such as health information and communication campaigns, fiscal measures such as taxes on sugar-sweetened beverages, direct restrictions and mandates, reformulation and improving the nutrient profile of food products, and standards regulating marketing to children can have significant and large impacts to improve diets and reduce the incidence of NCD. There is a need for more countries to implement population-based effective approaches to improve current diets.
ARE ASIAN AMERICANS WHO HAVE INTERRACIAL RELATIONSHIPS POLITICALLY DISTINCT?
Authors: Lemi Danielle Casarez , Kposowa Augustine
Published: March 2018

Research on interracial marriage and relationships uses the incidence of interracial romantic relationships to measure immigrant assimilation. Little attention, however, has been paid to the implications of interracial relationships for racial group politics. Are those who practice exogamy politically distinct from those who do not? We develop testable hypotheses from existing theories of and literature on interracial marriages/relationships. We test these hypotheses on several outcomes using the 2008 National Asian American Survey of Asian Americans, as this group has one of the highest rates of interracial marriage with Whites. We find that those with interracial partners aremorelikely to be concerned about racial issues,lesslikely to favor co-ethnic candidates and belong to ethnically concentrated civic groups, but are no more likely to be concerned about immigration or to favor a pathway to citizenship. We offer some theoretical reasons for these findings and discuss the implications of these findings for immigrant assimilation, interracial marriage, and the American racial order.
Potential for primary productivity in a globally-distributed bacterial phototroph
Authors: Graham E D , Heidelberg J F , Tully B J
Published: March 2018

Aerobic anoxygenic phototrophs (AAnPs) are common in marine environments and are associated with photoheterotrophic activity. To date, AAnPs that possess the potential for carbon fixation have not been identified in the surface ocean. Using the Tara Oceans metagenomic dataset, we have identified draft genomes of nine bacteria that possess the genomic potential for anoxygenic phototrophy, carbon fixation via the Calvin-Benson-Bassham cycle, and the oxidation of sulfite and thiosulfate. Forming a monophyletic clade within the Alphaproteobacteria and lacking cultured representatives, the organisms compose minor constituents of local microbial communities (0.1–1.0%), but are globally distributed, present in multiple samples from the North Pacific, Mediterranean Sea, the East Africa Coastal Province, and the Atlantic. This discovery may require re-examination of the microbial communities in the oceans to understand and constrain the role this group of organisms may play in the global carbon cycle.
Cytokine-induced killer cells as a feasible adoptive immunotherapy for the treatment of lung cancer
Authors: Chen Dan , Sha Huanhuan , Hu Tianmu , Dong Shuchen , Zhang Junying , Liu Siwen , Cao Haixia , Ma Rong , Wu Yang , Jing Changwen , Wang Zhuo , Wu Jianzhong , Feng Jifeng
Published: March 2018

Most of the patients with lung cancer are diagnosed at advanced stage, and they often lose the opportunity of surgical therapy, most of whom fail to reach good prognosis after chemotherapy. Recently, a few clinical studies have confirmed the role of adoptive T-cell transfer in the maintenance therapy of cancer patients. Here, we provided statistical insights into the role of CIKs in advanced lung cancer from three different levels, cell model (in vitro co-culture system), mice model (in situ lung cancer), and clinical research (in lung cancer patients of different progression stages). We optimized the components of supplements and cytokines on activating and expanding CIK cells. Based on this, we explored a new serum-free medium for in vitro activation and expansion of CIK cells. Moreover, we found that activated CIK cells could efficiently kill lung cancer cells in cell-to-cell model in vitro and significantly reduce the tumor growth in mice. For the clinical research, the OS rates of patients received combination of chemotherapy and CIK treatment were significantly improved compared to the OS rates of patients only received chemotherapy. Additionally, CIK therapy represented good toleration in our study. All the results suggested that combination of immunotherapy with traditional therapy will be a feasible and promising method for the treatment of lung cancer.
Wolfram syndrome: MAMs’ connection?
Authors: Delprat Benjamin , Maurice Tangui , Delettre Cécile
Published: March 2018

Wolfram syndrome (WS) is a rare neurodegenerative disease, the main pathological hallmarks of which associate with diabetes, optic atrophy, and deafness. Other symptoms may be identified in some but not all patients. Prognosis is poor, with death occurring around 35 years of age. To date, no treatment is available. WS was first described as a mitochondriopathy. However, the localization of the protein on the endoplasmic reticulum (ER) membrane challenged this hypothesis. ER contacts mitochondria to ensure effective Ca2+ transfer, lipids transfer, and apoptosis within stabilized and functionalized microdomains, termed “mitochondria-associated ER membranes” (MAMs). Two types of WS are characterized so far and Wolfram syndrome type 2 is due to mutation in CISD2, a protein mostly expressed in MAMs. The aim of the present review is to collect evidences showing that WS is indeed a mitochondriopathy, with established MAM dysfunction, and thus share commonalities with several neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis, as well as metabolic diseases, such as diabetes.
Evidence for ephemeral middle Eocene to early Oligocene Greenland glacial ice and pan-Arctic sea ice
Authors: Tripati Aradhna , Darby Dennis
Published: March 2018

Earth’s modern climate is defined by the presence of ice at both poles, but that ice is now disappearing. Therefore understanding the origin and causes of polar ice stability is more critical than ever. Here we provide novel geochemical data that constrain past dynamics of glacial ice on Greenland and Arctic sea ice. Based on accurate source determinations of individual ice-rafted Fe-oxide grains, we find evidence for episodic glaciation of distinct source regions on Greenland as far-ranging as ~68°N and ~80°N synchronous with ice-rafting from circum-Arctic sources, beginning in the middle Eocene. Glacial intervals broadly coincide with reduced CO2, with a potential threshold for glacial ice stability near ~500 p.p.m.v. The middle Eocene represents the Cenozoic onset of a dynamic cryosphere, with ice in both hemispheres during transient glacials and substantial regional climate heterogeneity. A more stable cryosphere developed at the Eocene-Oligocene transition, and is now threatened by anthropogenic emissions.
Insulin mediates de novo nuclear accumulation of the IGF-1/insulin Hybrid Receptor in corneal epithelial cells
Authors: Titone Rossella , Zhu Meifang , Robertson Danielle M.
Published: March 2018

Insulin and insulin-like growth factor-1 (IGF-1) are present in human tears and likely play an important role in mediating ocular surface homeostasis. We previously characterized the IGF-1/insulin hybrid receptor (Hybrid–R) in corneal epithelial cells and found that it was activated by IGF-1 and not insulin; and reported the novel finding that it localized to the corneal epithelial cell nucleus. Since the corneal epithelium is an insulin insensitive tissue and does not require insulin for glucose uptake, this study investigated the function of insulin in corneal epithelial cells. We show that stress induced by growth factor deprivation triggers transcriptional upregulation and de novo nuclear accumulation of Hybrid-R through the homodimeric insulin receptor (INSR). This occurs independent of PI3K/Akt signaling. Nuclear accumulation of Hybrid-R was associated with partial cell cycle arrest and a corresponding reduction in mitochondrial respiration. Treatment with insulin, and not IGF-1, attenuated IGF-1R and INSR transcription and restored cell cycle and metabolic homeostasis. Together, these findings support that insulin mediates receptor homeostasis in corneal epithelial cells, favoring an IGF-1 mediated pathway. This may have important implications in diabetic corneal disease and wound healing.
Genetic analyses in a bonobo (Pan paniscus) with arrhythmogenic right ventricular cardiomyopathy
Authors: Celestino-Soper Patrícia B. S. , Lynnes Ty C. , Zhang Lili , Ouyang Karen , Wann Samuel , Clyde Victoria L. , Vatta Matteo
Published: March 2018

Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a disorder that may lead to sudden death and can affect humans and other primates. In 2012, the alpha male bonobo of the Milwaukee County Zoo died suddenly and histologic evaluation found features of ARVC. This study sought to discover a possible genetic cause for ARVC in this individual. We sequenced our subject’s DNA to search for deleterious variants in genes involved in cardiovascular disorders. Variants found were annotated according to the human genome, following currently available classification used for human diseases. Sequencing from the DNA of an unrelated unaffected bonobo was also used for prediction of pathogenicity. Twenty-four variants of uncertain clinical significance (VUSs) but no pathogenic variants were found in the proband studied. Further familial, functional, and bonobo population studies are needed to determine if any of the VUSs or a combination of the VUSs found may be associated with the clinical findings. Future genotype-phenotype establishment will be beneficial for the appropriate care of the captive zoo bonobo population world-wide as well as conservation of the bobono species in its native habitat.
Swelling Inhibition of Liquid Crystalline Colloidal Montmorillonite and Beidellite Clays by DNA
Authors: Yamaguchi Naoya , Anraku Shinya , Paineau Erwan , Safinya Cyrus R. , Davidson Patrick , Michot Laurent J. , Miyamoto Nobuyoshi
Published: March 2018

Exploring the interaction of nucleic acids with clay minerals is important to understand such issues as the persistence in soils of biomolecules and the appearance of genetic polymers in prebiotic environments. Colloidal dispersions of double stranded DNA and clay nanosheets may also provide interesting model systems to study the statistical physics of mixtures of semi-flexible rods and plates. Here, we show that adding very small amounts of DNA to liquid-crystalline montmorillonite and beidellite smectite clay suspensions strongly widens the isotropic/nematic phase coexistence region. Moreover, a spectroscopic study shows that, upon DNA addition, the first DNA molecules adsorb onto the clay particles. Remarkably, synchrotron small-angle X-ray scattering experiments reveal that the average distance between the clay sheets, in the nematic phase at coexistence, decreases with increasing DNA concentration and that the inhibition of swelling by DNA becomes almost independent of clay concentration. We interpret this DNA-mediated attraction between clay nanosheets by bridging conformations of DNA strands (plates on a string structure). In addition to bridging, DNA chains can form “loops” between sections adsorbed on the same particle, giving rise to sheet repulsions due to protruding loops. This interpretation agrees with the observed inter-clay spacings being dependent only on the DNA concentration.
Using massively parallel shotgun sequencing of maternal plasmatic cell-free DNA for cytomegalovirus DNA detection during pregnancy: a proof of concept study
Authors: Chesnais Virginie , Ott Alban , Chaplais Emmanuel , Gabillard Samuel , Pallares Diego , Vauloup-Fellous Christelle , Benachi Alexandra , Costa Jean-Marc , Ginoux Eric
Published: March 2018

Human cytomegalovirus (HCMV) primary infections of pregnant women can lead to congenital infections of the fetus that could have severe impacts on the health of the newborn. Recent studies have shown that 10–100 billion DNA fragments per milliliter of plasma are circulating cell-free. The study of this DNA has rapidly expanding applications to non-invasive prenatal testing (NIPT). In this study, we have shown that we can detect viral specific reads in the massively parallel shotgun sequencing (MPSS) NIPT data. We have also observed a strong correlation between the viral load of calibration samples and the number of reads aligned on the reference genome. Based on these observations we have constructed a statistical model able to quantify the viral load of patient samples. We propose to use this new method to detect and quantify circulating DNA virus like HCMV during pregnancy using the same sequencing results as NIPT data. This method could be used to improve the NIPT diagnosis.
Natural turn measures predict recurrent falls in community-dwelling older adults: a longitudinal cohort study
Authors: Leach Julia M. , Mellone Sabato , Palumbo Pierpaolo , Bandinelli Stefania , Chiari Lorenzo
Published: March 2018

Although turning has been reported as one of the leading activities performed during a fall, and falls during turning result in 8-times more hip fractures than falls during linear gait, the quantity and quality of turns resulting in falls remain unknown since turns are rarely assessed during activities of daily living. 160 community-dwelling older adults were monitored for one week using smartphone technology. Turn measures and activity rates were quantified. Fall incidence within 12 months from continuous monitoring defined fall status, with 7/153 prospective fallers/non-fallers. Based on the analysis of 718,582 turns, prospective fallers turned less frequently, took longer to turn, and were less consistent in turn angle (p = 0.007, 0.025, and 0.038, respectively). Prospective fallers also walked slower and spent less time walking and turning and more time engaged in sedentary behavior (p = 0.043, 0.012, and 0.015, respectively). Individuals experiencing decline in the control of gait and/or turning may attempt to reduce their risk of falling by limiting their exposure and implementing cautionary movement strategies while turning. Since there was no difference in the overall active rate between prospective fallers and non-fallers, impaired gait and turning ability, specifically, may attribute to elevated fall risk within this cohort.
Proteome-wide mapping of immune features onto Plasmodium protein three-dimensional structures
Authors: Guy Andrew J. , Irani Vashti , Beeson James G. , Webb Benjamin , Sali Andrej , Richards Jack S. , Ramsland Paul A.
Published: March 2018

Humoral immune responses against the malaria parasite are an important component of a protective immune response. Antibodies are often directed towards conformational epitopes, and the native structure of the antigenic region is usually critical for antibody recognition. We examined the structural features of various Plasmodium antigens that may impact on epitope location, by performing a comprehensive analysis of known and modelled structures from P. falciparum. Examining the location of known polymorphisms over all available structures, we observed a strong propensity for polymorphic residues to be exposed on the surface and to occur in particular secondary structure segments such as hydrogen-bonded turns. We also utilised established prediction algorithms for B-cell epitopes and MHC class II binding peptides, examining predicted epitopes in relation to known polymorphic sites within structured regions. Finally, we used the available structures to examine polymorphic hotspots and Tajima’s D values using a spatial averaging approach. We identified a region of PfAMA1 involving both domains II and III under a high degree of balancing selection relative to the rest of the protein. In summary, we developed general methods for examining how sequence-based features relate to one another in three-dimensional space and applied these methods to key P. falciparum antigens.
Cost-effective generation of precise label-free quantitative proteomes in high-throughput by microLC and data-independent acquisition
Authors: Vowinckel Jakob , Zelezniak Aleksej , Bruderer Roland , Mülleder Michael , Reiter Lukas , Ralser Markus
Published: March 2018

Quantitative proteomics is key for basic research, but needs improvements to satisfy an increasing demand for large sample series in diagnostics, academia and industry. A switch from nanoflowrate to microflowrate chromatography can improve throughput and reduce costs. However, concerns about undersampling and coverage have so far hampered its broad application. We used a QTOF mass spectrometer of the penultimate generation (TripleTOF5600), converted a nanoLC system into a microflow platform, and adapted a SWATH regime for large sample series by implementing retention time- and batch correction strategies. From 3 µg to 5 µg of unfractionated tryptic digests that are obtained from proteomics-typical amounts of starting material, microLC-SWATH-MS quantifies up to 4000 human or 1750 yeast proteins in an hour or less. In the acquisition of 750 yeast proteomes, retention times varied between 2% and 5%, and quantified the typical peptide with 5–8% signal variation in replicates, and below 20% in samples acquired over a five-months period. Providing precise quantities without being dependent on the latest hardware, our study demonstrates that the combination of microflow chromatography and data-independent acquisition strategies has the potential to overcome current bottlenecks in academia and industry, enabling the cost-effective generation of precise quantitative proteomes in large scale.
Psychophysical Laws and the Superorganism
Authors: Reina Andreagiovanni , Bose Thomas , Trianni Vito , Marshall James A. R.
Published: March 2018

Through theoretical analysis, we show how a superorganism may react to stimulus variations according to psychophysical laws observed in humans and other animals. We investigate an empirically-motivated honeybee house-hunting model, which describes a value-sensitive decision process over potential nest-sites, at the level of the colony. In this study, we show how colony decision time increases with the number of available nests, in agreement with the Hick-Hyman law of psychophysics, and decreases with mean nest quality, in agreement with Piéron’s law. We also show that colony error rate depends on mean nest quality, and difference in quality, in agreement with Weber’s law. Psychophysical laws, particularly Weber’s law, have been found in diverse species, including unicellular organisms. Our theoretical results predict that superorganisms may also exhibit such behaviour, suggesting that these laws arise from fundamental mechanisms of information processing and decision-making. Finally, we propose a combined psychophysical law which unifies Hick-Hyman’s law and Piéron’s law, traditionally studied independently; this unified law makes predictions that can be empirically tested.
Time-lapse observation of stepwise regression of Erk activity in zebrafish presomitic mesoderm
Authors: Sari Dini Wahyu Kartika , Akiyama Ryutaro , Naoki Honda , Ishijima Hannosuke , Bessho Yasumasa , Matsui Takaaki
Published: March 2018

During somite segmentation, clock genes oscillate within the posterior presomitic mesoderm (PSM). The temporal information ties up with the posteriorly moving FGF gradient, leading to the formation of a presumptive somite within the PSM. We previously investigated Erk activity downstream of FGF signaling by collecting stained zebrafish embryos, and discovered that the steep gradient of Erk activity was generated in the PSM, and the Erk activity border regularly shifted in a stepwise manner. However, since these interpretations come from static analyses, we needed to firmly confirm them by applying an analysis that has higher spatiotemporal resolutions. Here we developed a live imaging system for Erk activity in zebrafish embryos, using a Förster resonance energy transfer (FRET)-based Erk biosensor. With this system, we firmly showed that Erk activity exhibits stepwise regression within the PSM. Although our static analyses could not detect the stepwise pattern of Erk activity in clock-deficient embryos, our system revealed that, in clock-deficient embryos, the stepwise regression of Erk activity occurs at an irregular timing, eventually leading to formation of irregularly-sized somites. Therefore, our system overcame the limitation of static analyses and revealed that clock-dependent spatiotemporal regulation of Erk is required for proper somitogenesis in zebrafish.
Author Correction: Influence of soil moisture on codenitrification fluxes from a urea-affected pasture soil
Authors: Clough Timothy J. , Lanigan Gary J. , de Klein Cecile A. M. , Samad Md. Sainur , Morales Sergio E. , Rex David , Bakken Lars R. , Johns Charlotte , Condron Leo M. , Grant Jim , Richards Karl G.
Published: March 2018

A correction has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
Distribution of brain sodium long and short relaxation times and concentrations: a multi-echo ultra-high field 23Na MRI study
Authors: Ridley Ben , Nagel Armin M. , Bydder Mark , Maarouf Adil , Stellmann Jan-Patrick , Gherib Soraya , Verneuil Jeremy , Viout Patrick , Guye Maxime , Ranjeva Jean-Philippe , Zaaraoui Wafaa
Published: March 2018

Sodium (23Na) MRI proffers the possibility of novel information for neurological research but also particular challenges. Uncertainty can arise in in vivo23Na estimates from signal losses given the rapidity of T2* decay due to biexponential relaxation with both short (T2*short) and long (T2*long) components. We build on previous work by characterising the decay curve directly via multi-echo imaging at 7 T in 13 controls with the requisite number, distribution and range to assess the distribution of both in vivo T2*short and T2*long and in variation between grey and white matter, and subregions. By modelling the relationship between signal and reference concentration and applying it to in vivo23Na-MRI signal, 23Na concentrations and apparent transverse relaxation times of different brain regions were measured for the first time. Relaxation components and concentrations differed substantially between regions of differing tissue composition, suggesting sensitivity of multi-echo 23Na-MRI toward features of tissue composition. As such, these results raise the prospect of multi-echo 23Na-MRI as an adjunct source of information on biochemical mechanisms in both physiological and pathophysiological states.
Erosion of natural darkness in the geographic ranges of cacti
Authors: Correa-Cano Maria Eugenia , Goettsch Bárbara , Duffy James P. , Bennie Jonathan , Inger Richard , Gaston Kevin J.
Published: March 2018

Naturally dark nighttime environments are being widely eroded by the introduction of artificial light at night (ALAN). The biological impacts vary with the intensity and spectrum of ALAN, but have been documented from molecules to ecosystems. How globally severe these impacts are likely to be depends in large part on the relationship between the spatio-temporal distribution of ALAN and that of the geographic ranges of species. Here, we determine this relationship for the Cactaceae family. Using maps of the geographic ranges of cacti and nighttime stable light composite images for the period 1992 to 2012, we found that a high percentage of cactus species were experiencing ALAN within their ranges in 1992, and that this percentage had increased by 2012. For almost all cactus species (89.7%) the percentage of their geographic range that was lit increased from 1992–1996 to 2008–2012, often markedly. There was a significant negative relationship between the species richness of an area, and that of threatened species, and the level of ALAN. Cacti could be particularly sensitive to this widespread and ongoing intrusion of ALAN into their geographic ranges, especially when considering the potential for additive and synergistic interactions with the impacts of other anthropogenic pressures.