bluuebunny/crossref_metadata_2025 huggingface dataset:
AKNA Frameshift Variant in Three Dogs with Recurrent Inflammatory Pulmonary Disease
Authors: Hug Petra , Anderegg Linda , Kehl Alexandra , Jagannathan Vidhya , Leeb Tosso
Published: July 2019

We investigated three related Rough Collies with recurrent inflammatory pulmonary disease. The clinical symptoms were similar to primary ciliary dyskinesia (PCD). However, the affected dogs did not carry any known pathogenic PCD variants. Pedigree analysis suggested a recessive mode of inheritance. Combined linkage and homozygosity mapping in three cases and seven non-affected family members delineated 19 critical intervals on 10 chromosomes comprising a total of 99 Mb. The genome of one affected dog was sequenced and compared to 601 control genomes. We detected only a single private homozygous protein-changing variant in the critical intervals. The detected variant was a 4 bp deletion, c.2717_2720delACAG, in the AKNA gene encoding the AT-hook transcription factor. It causes a frame-shift introducing a premature stop codon and truncates 37% of the open reading frame, p.(Asp906Alafs*173). We genotyped 88 Rough Collies consisting of family members and unrelated individuals. All three available cases were homozygous for the mutant allele and all 85 non-affected dogs were either homozygous wildtype (n = 67) or heterozygous (n = 18). AKNA modulates inflammatory immune responses. Akna−/− knockout mice die shortly after birth due to systemic autoimmune inflammatory processes including lung inflammation that is accompanied by enhanced leukocyte infiltration and alveolar destruction. The perfect genotype-phenotype association and the comparative functional data strongly suggest that the detected AKNA:c.2717_2720delACAG variant caused the observed severe airway inflammation in the investigated dogs. Our findings enable genetic testing, which can be used to avoid the unintentional breeding of affected puppies.
Kinetic Modeling of DUSP Regulation in Herceptin-Resistant HER2-Positive Breast Cancer
Authors: Buiga Petronela , Elson Ari , Tabernero Lydia , Schwartz Jean-Marc
Published: July 2019

Background: HER2 (human epidermal growth factor 2)-positive breast cancer is an aggressive type of breast cancer characterized by the overexpression of the receptor-type protein tyrosine kinase HER2 or amplification of the HER2 gene. It is commonly treated by the drug trastuzumab (Herceptin), but resistance to its action frequently develops and limits its therapeutic benefit. Dual-specificity phosphatases (DUSPs) were previously highlighted as central regulators of HER2 signaling; therefore, understanding their role is crucial to designing new strategies to improve the efficacy of Herceptin treatment. We investigated whether inhibiting certain DUSPs re-sensitized Herceptin-resistant breast cancer cells to the drug. We built a series of kinetic models incorporating the key players of HER2 signaling pathways and simulating a range of inhibition intensities. The simulation results were compared to live tumor cells in culture, and showed good agreement with the experimental analyses. In particular, we observed that Herceptin-resistant DUSP16-silenced breast cancer cells became more responsive to the drug when treated for 72 h with Herceptin, showing a decrease in resistance, in agreement with the model predictions. Overall, we showed that the kinetic modeling of signaling pathways is able to generate predictions that assist experimental research in the identification of potential targets for cancer treatment.
Copper and its Isotopes in Organic-Rich Sediments: From the Modern Peru Margin to Archean Shales
Authors: Ciscato Emily R. , Bontognali Tomaso R. R. , Poulton Simon W. , Vance Derek
Published: July 2019

The cycling of copper (Cu) and its isotopes in the modern ocean is controlled by the interplay of biology, redox settings, and organic complexation. To help build a robust understanding of Cu cycling in the modern ocean and investigate the potential processes controlling its behavior in the geological past, this study presents Cu abundance and isotope data from modern Peru Margin sediments as well as from a suite of ancient, mostly organic-rich, shales. Analyses of an organic-pyrite fraction extracted from bulk modern sediments suggest that sulphidation is the main control on authigenic Cu enrichments in this setting. This organic-pyrite fraction contains, in most cases, >50% of the bulk Cu reservoir. This is in contrast to ancient samples, for which a hydrogen fluoride (HF)-dissolvable fraction dominates the total Cu reservoir. With <20% of Cu found in the organic-pyrite fraction of most ancient sediments, interpretation of the associated Cu isotope composition is challenging, as primary signatures may be masked by secondary processes. But the Cu isotope composition of the organic-pyrite fraction in ancient sediments hints at the potential importance of a significant Cu(I) reservoir in ancient seawater, perhaps suggesting that the ancient ocean was characterized by different redox conditions and a different Cu isotope composition to that of the modern ocean.
Psychological Processes and Personality Factors for an Appropriate Tsunami Evacuation
Authors: Sugiura Motoaki , Sato Shosuke , Nouchi Rui , Honda Akio , Ishibashi Ryo , Abe Tsuneyuki , Muramoto Toshiaki , Imamura Fumihiko
Published: July 2019

Although various factors related to the environment (perception of earthquake and warning) and knowledge (oral history and scientific knowledge) affect individual differences in evacuation behavior before a tsunami, the roles of psychological processes and personality factors in such relationships are poorly understood. We addressed this research gap by applying hierarchical regression analyses to survey data from survivors of the 2011 Tohoku earthquake and tsunami disaster. Previously-known contributions of environmental and knowledge factors were mostly replaced by the perception of a tsunami risk and threat, and these background factors were shown to facilitate these psychological processes. Several personality factors directly contributed to voluntary evacuation, particularly leadership and active well-being in the Power to Live scale, and extraversion in the Big Five scale. Overall, these results seem to indicate the need for formulating two independent targets when developing measures to enhance appropriate tsunami evacuation. Facilitation of the perception of a tsunami risk and threat may basically be pursued by developing existing approaches. Addressing the relevant personality factors may be done in a broader context of disaster or general education and sociocultural activities.
Acoustic and Microstructural Properties of Partially Molten Samples in the Ice–Ammonia System
Authors: McCarthy Christine , Nielson Michael A. , Coonin Allie N. , Minker Jessica Shea , Domingos Armando A.
Published: July 2019

We measured the ultrasonic properties and microstructure of two-phase binary mixtures of the ice–ammonia partial melt system, which was selected based on its importance for numerous planetary bodies. The equilibrium microstructure of ice–ammonia melt was examined using a light microscope within a cold room. The measured median dihedral angle between the solid and melt at 256 K is approximately 63°, with a broad distribution of observed angles between 10° and 130°. P-wave velocities in the partially molten samples were measured as a function of temperature (177 < T(K) < 268) and composition (1–6.4 wt % NH3). Vp decreases approximately linearly with increasing temperature and melt fraction. We compare the results of this study to those of other potential binary systems by normalizing the datasets using a vertical lever (TL–TE) and articulating the potential effects on the mechanical behavior and transport capabilities of partially molten ice in icy satellites.
Frost Resilience of Stabilized Earth Building Materials
Authors: Rempel Alan W. , Rempel Alexandra R.
Published: July 2019

Earth-based building materials are increasingly valued in green design for their low embodied energy, humidity-buffering ability, and thermal stability. These materials perform well in warm dry climates, but greater understanding of long-term durability is needed for successful adoption in colder and/or wetter climates. The presence of stabilizers dramatically improves resistance to surface erosion from wind and rain, compared to unstabilized adobe and cob counterparts, and the influences of soil composition, fiber type, and diverse binders, on rain and wind surface erosion have been investigated in detail. Frost and freeze-thaw resistance, however, have been less well-studied, despite strong interest in stabilized earth materials in northern North America, Europe, and Asia. In particular, recent studies have relied on a widespread misunderstanding of the mechanism by which frost damage occurs in porous materials that will impede efforts to create valid models for material design and improvement. In addition, the influence of radiative thermal stresses on wall surfaces has been overlooked in favor of focus on ambient air temperatures. Here, we apply contemporary understanding of cracking by segregated ice growth to develop a macroscopic damage index that enables comparison between performance of different materials subject to different weather patterns. An examination of predicted damage patterns for two stabilized earth building materials and two conventional materials in twelve cities over two time periods reveals the dominant factors that govern frost vulnerability. We find that the frost resilience of earth building materials is comparable to that of the conventional materials we examined, and that assessments that neglect expected variations in water content by assuming full saturation are likely to yield misleading results. Over recent years, increased winter temperatures in several cities we examined predict reduced material vulnerability to frost damage, but we also find that accompanying increases in humidity levels have made some cities much more vulnerable.
A Quick-Look Method for Initial Evaluation of Gas Hydrate Stability below Subaqueous Permafrost
Authors: Tinivella Umberta , Giustiniani Michela , Marín-Moreno Héctor
Published: July 2019

Many studies demonstrated the coexistence of subaqueous permafrost and gas hydrate. Subaqueous permafrost could be a factor affecting the formation/dissociation of gas hydrate. Here, we propose a simple empirical approach that allows estimating the steady-state conditions for gas hydrate stability in the presence of subaqueous permafrost. This approach was derived for pressure, temperature, and salinity conditions typical of subaqueous permafrost in marine (brine) and lacustrine (freshwater) environments.
Judith and Holofernes: Reconstructing the History of a Painting Attributed to Artemisia Gentileschi
Authors: Impallaria Anna , Petrucci Ferruccio , Bruno Simone
Published: July 2019

Recently, a new painting attributed to Artemisia Gentileschi was found in Ferrara, representing Judith exposing the head of Holofernes. Some analyses have been required to verify the history of this canvas, because another known painting is very similar to this one with the exception of the heads of Judith and Holofernes. This last has been attributed to the father of Artemisia, Orazio Gentileschi. Many diagnostics were performed, starting from imaging techniques: from raking light, to UV fluorescence and X-ray radiography. All of them highlighted peculiarities concerning above all the head of the main female protagonist. The results suggest that the face of Judith was subjected to various reworks in the same artistic period because of the original materials still present. This is the reason for the peculiar fragility and, due to this, the restoration of the 20th Century focused on Judith’s face. However, in this contribution, we want to highlight the results obtained with XRF spot analysis. Indeed, the artistic palette and the restoration materials have been characterized. For example, reds are in cinnabar, while Judith’s lips have been restored with cadmium red. The more interesting results regard the use of umber earths. In the painting, this iron-based pigment, rich in manganese, was revealed several times, and the correlation between Fe and Mn was easily verified. More than one correlation has been found due to the use of this pigment to darken the hues.
Tobacco Control Policy in Scotland: A Qualitative Study of Expert Views on Successes, Challenges and Future Actions
Authors: Laird Yvonne , Myers Fiona , Reid Garth , McAteer John
Published: July 2019

The Scottish Government launched a tobacco control strategy in 2013 with the ambition of making Scotland tobacco smoke-free by 2034. However, 17% of the adult population in Scotland smoke cigarettes. This study aimed to provide insight into why policies are successful or not and provide suggestions for future policy actions. Individual interviews with ten tobacco control experts were conducted and the results were analyzed using thematic analysis. Key successes included strong political leadership, mass media campaigns, legislation to address availability and marketing of cigarettes and tobacco products, and legislation to reduce second-hand smoke exposure. Challenges included implementing policy actions, monitoring and evaluation of tobacco control actions, addressing health inequalities in smoking prevalence, and external factors that influenced the success of policy actions. Key suggestions put forward for future policy actions included addressing the price and availability of tobacco products, maintaining strong political leadership on tobacco control, building on the success of the ‘Take it Right Outside’ mass media campaign with further mass media campaigns to tackle other aspects of tobacco control, and developing and testing methods of addressing inequalities in cigarette smoking prevalence. The findings of this study can inform future tobacco control policy in Scotland and have relevance for tobacco control policies in other countries.
Evaluating the Provision of Health Services and Barriers to Treatment for Chronic Diseases among Syrian Refugees in Turkey: A Review of Literature and Stakeholder Interviews
Authors: Alawa Jude , Zarei Parmida , Khoshnood Kaveh
Published: July 2019

Background: While Turkey hosts the largest number of Syrian refugees, the provision of health services for chronic disease among Syrian refugees in Turkey has been inadequate and understudied. This paper explores Turkish healthcare policies surrounding Syrian refugees’ access to health services for chronic diseases. Methods: We conducted a literature review and supplementary stakeholder interviews to evaluate the provision of chronic health services and the most common barriers to healthcare access among Syrian refugees in Turkey. Results: Though access to treatment for displaced Syrians has improved throughout the past five years, five primary barriers persist: registration procedure regulations, navigation of a new health system, language barriers, fear of adverse treatment, and cost. Conclusions: To drive improvements in healthcare for chronic diseases among Syrian refugees in Turkey, we recommend making registration procedures more accessible, developing more healthcare options in patients’ native language, increasing human resources, and advocating for more research surrounding chronic health conditions among refugees.
Regional Temperature Response in Central Asia to National Committed Emission Reductions
Authors: Zhang Jintao , Wang Fang
Published: July 2019

National committed greenhouse gas emission reduction actions are the center of the Paris Agreement, and are known as ‘Intended Nationally Determined Contributions’ (INDC) that aim to slow down global warming. The climate response to INDC emission reduction is a focus in climate change science. In this study, data from 32 global climate models from the Coupled Model Intercomparison Project Phase 5 (CMIP5) were applied to investigate the changes in the mean and extreme high temperatures in Central Asia (CA) under the INDC scenario above the present-day level. The results show that the magnitude of warming in CA is remarkably higher than the global mean. Almost all the regions in CA will experience more intense, more frequent, and longer-lasting extreme high-temperature events. In comparison with the INDC scenario, the reduced warming of the 2.0 °C/1.5 °C target scenarios will help avoid approximately 44–61%/65–80% of the increase in extreme temperature events in terms of the intensity, frequency, and duration in CA. These results contribute to an improved understanding of the benefits of limiting global warming to the 2.0 °C/1.5 °C targets, which is paramount for mitigation and adaptation planning.
Comparison of a Wearable Tracker with Actigraph for Classifying Physical Activity Intensity and Heart Rate in Children
Authors: Kang Seoungki , Kim Youngdeok , Byun Wonwoo , Suk JinSu , Lee Jung-Min
Published: July 2019

Introduction: To examine the validity and reliability of the Fitbit Charge HR (FCH), wrist-worn ActiGraph (AG) accelerometers were used for assessing the classification of physical activity (PA) into intensity categories in children. Methods: Forty-three children (n = 43) participated in the study. Each participant completed 3 min bouts of 12 PAs ranging from sedentary to vigorous intensity while simultaneously wearing FCH and AG on both hands, a Polar HR monitor, and a portable indirect calorimeter. Total time spent in different PA intensity levels measured by FCH and AG were compared to the indirect calorimetry. Results: The highest classification accuracy values of sedentary behavior was 81.1% for FCH. The highest classification (72.4%) of light intensity PA was observed with Crouter’s algorithm from the non-dominant wrist. Crouter’s algorithm also show the highest classification (81.8%) for assessing moderate to vigorous intensity PA compared to FCH (70.8%). Across the devices, a high degree of reliability was found in step measurements, ranging from an intra-class correlation (ICC) = 0.92 to an ICC = 0.94. The reliability of the AG and the FCH showed high agreement for each variable. Conclusion: The FCH shows better validity for estimating sedentary behavior and similar validity for assessing moderate to vigorous PA compared to the research-grade monitor. Across the devices, the reliability showed the strongest association.
Application of the Spline Interpolation in Simulating the Distribution of Phytoplankton in a Marine NPZD Type Ecosystem Model
Authors: Li Xiaona , Zheng Quanxin , Lv Xianqing
Published: July 2019

The available observations for the model are usually sparse and uneven. The application of interpolation methods help researchers obtain an approximate form of the original data. A marine nutrient, phytoplankton, zooplankton and detritus (NPZD) type ecosystem model is applied to simulate the distribution of phytoplankton combined with the spline interpolation (SI) and the Cressman interpolation (CI). In the idealized twin experiments, the performance of these two interpolation methods is validated through the analysis of several quantitative metrics, which show the minor error and high efficiency when using the SI. Namely, the given distributions can be better inverted with the SI. The actual distribution of phytoplankton in the Bohai Sea is interpolated in the practical experiment, where a satisfactory simulation result is obtained by the model with the SI. The model experiments and results verify the feasibility and effectiveness of SI.
Effects of Visual and Auditory Perturbations on Ski-Specific Balance among Males and Females—A Randomized Crossover Trial
Authors: Niedermeier Martin , Pocecco Elena , Hildebrandt Carolin , Raschner Christian , Federolf Peter , Kopp Martin , Ruedl Gerhard
Published: July 2019

Injuries in skiing show sex-specific differences, especially when visual perception is reduced. Reduced visual perception impairs balance, which plays an important role in avoiding skiing injuries. However, males and females might cope differently with reduced visual perception. Thus, the aim of this study was to evaluate sex-related effects of environmental perturbations (reduced visual perception and listening to music) on ski-specific balance. Using a crossover design, ski-specific balance was tested in 50 young adults (50% female) in four conditions: with and without listening to music and/or with and without reduced visual perception (ski goggles with occlusion foil). A four × two (condition by sex) mixed ANOVA revealed a significant condition by sex interaction, partial η² = 0.06. Females showed an increase in balance from the condition without music/with normal visual perception to the condition with music/with normal visual perception, while males showed a decrease. Balance was significantly higher in females compared to males, partial η² = 0.31. The findings suggest that balance is affected differently by environmental perturbations in females and males. However, the differences observed were not in line with our initial hypotheses, which might be because the model was too simplistic for how visual/auditory perturbations may affect balance.
Psychosocial Factors Associated with Quality of Life in Young Men Who Have Sex with Men Living with HIV/AIDS in Zhejiang, China
Authors: Jiang Tingting , Zhou Xin , Wang Hui , Luo Mingyu , Pan Xiaohong , Ma Qiaoqin , Chen Lin
Published: July 2019

Objectives: To explore the quality of life (QOL) status and related factors in young human immunodeficiency virus (HIV)-infected men who have sex with men (MSM) aged 16 to 24 years in Zhejiang province. Methods: A cross-sectional study was conducted in 22 counties of Zhejiang province, and 395 subjects took part in our research. A t-test, one-way Analysis of variance (ANOVA), and multivariate stepwise linear regression analysis were used to investigate the factors associated with QOL in young HIV-infected MSM. Results: The total score on the QOL was 86.86 ± 14.01. The multivariate stepwise linear regression analysis revealed that self-efficacy and discrimination were associated with all domains on the QOL assessment, monthly income was associated with QOL for all domains except spirituality and consistent condom use during oral sex with men in the past three months was associated with QOL for all domains except the relationship domain. Those individuals within the group of young HIV-infected MSM who have higher self-efficacy, a higher monthly income, greater social support, safer sexual behaviors, a higher level of education, and a higher cluster of differentiation 4 (CD4) count have a better QOL. Conclusions: These findings suggest that to improve the QOL of this population, greater emphasis should be placed on improving social support, self-efficacy, and antiviral therapy adherence and on reducing discrimination, disease progression, and high-risk behaviors.
Breakfast Nutritional Quality and Cognitive Interference in University Students from Mexico City
Authors: Sámano Reyna , Hernández-Chávez Carmen , Chico-Barba Gabriela , Córdova-Barrios Armando , Morales-del-Olmo Mayela , Sordo-Figuero Hortensia , Hernández Miguel , Merino-Palacios Carmen , Cervantes-Zamora Lucero , Martínez-Rojano Hugo
Published: July 2019

Skipping breakfast might have an impact on cognitive functions, such as interference, which is a basic capacity of executive functions that denotes the possibility of controlling an automated response. This study aimed to analyze the association between nutritional quality of breakfast and cognitive interference in a sample of university students. A cross-sectional study was conducted, a total of 422 students between 18 and 25 years participated. Cognitive interference was assessed with the Stroop Test. Breakfast was assessed with a questionnaire assigning a score for each serving of each food group that was consumed. Logistic regression models were performed. The performance in cognitive tasks was slower in those who had a poor breakfast (32.9 ± 6 vs 29.3 ± 6 s, p < 0.050). Poor cognitive interference was greater in students with poor breakfast (53% versus 23%, p = 0.001). A slower word reading was associated with skipping vegetables (OR: 2.78, 95% CI: 0.09–2.13), and cereals (OR: 1.70, 95% CI: 1.03–2.81). Wrong color identification was associated with skipping fruits (OR: 1.55, 95% CI: 1.43–1.99) and animal protein sources (OR: 1.63, 95% CI: 1.07–2.49). Skipping fat-rich cereals was a protector factor (OR: 0.55, 95% CI: 0.36–0.85). Difficulty in the ability to inhibit interference was associated with skipping vegetables (OR: 2.72, 95% CI: 1.25–4.80) and cereals (OR: 2.65, 95% CI: 1.28–4.68). The nutritional quality of breakfast was associated with the time spent answering the Stroop test, but not with cognitive interference.
Gender Discrimination in the Workplace: Effects on Pregnancy Planning and Childbirth among South Korean Women
Authors: Kim Ji-Hye , Oh Sarah Soyeon , Bae Suk Won , Park Eun-Cheol , Jang Sung-In
Published: July 2019

Introduction: This study aims to investigate the association between gender discrimination in the workplace and pregnancy planning/childbirth experiences among working women in South Korea. Methods: We analyzed data from the Korean Longitudinal Survey of Women and Families (KLoWF) for the years 2007 to 2016. The study population consisted of 7996 working women, between the ages of 19 and 45. Gender discrimination was measured through the 6-item Workplace Gender Discrimination Scale, evaluating discrimination in terms of recruitment, promotions, pay, deployment, training and lay-offs. Multiple logistic regression analysis was employed to measure the association between gender discrimination and the pregnancy planning/childbirth experience. Results: Compared to individuals experiencing no discrimination in the workplace, those experiencing low [odds ratio (OR): 0.78, 95% confidence interval (95% CI) 0.61–0.99] or medium (OR: 0.69, 95% CI: 0.54–0.89) levels of discrimination had decreased odds of pregnancy planning. Likewise, individuals scoring low (OR: 0.70, 95% CI 0.54–0.92), medium (OR: 0.68, 95% CI: 0.51–0.92), or high (OR: 0.47, 95% CI: 0.27–0.80) levels of discrimination also had decreased odds of childbirth experience when compared to the no-experience group. When stratified by income, compared to individuals experiencing no discrimination in the workplace, those experiencing gender discrimination had decreased odds of pregnancy planning for low income (low OR: 0.64, 95% CI: 0.45–0.92; medium OR: 0.55, 95% CI: 0.52–0.97; high OR: 0.45, 95% CI: 0.24–0.87), medium income (medium OR: 0.53, 95% CI: 0.37–0.77; high OR: 0.29, 95% CI: 0.14–0.63), and high income groups (low OR: 0.64, 95% CI: 0.49–0.84; medium OR: 0.69, 95% CI: 0.52–0.92). Conclusions: The present study finds that gender discrimination in the workplace is associated with decreased odds of pregnancy planning/childbirth experience among working South Korean women. Furthermore, low and medium income groups were especially more likely to be affected by the level of gender discrimination in the workplace when planning pregnancy.
Whitefly Control Strategies against Tomato Leaf Curl New Delhi Virus in Greenhouse Zucchini
Authors: Rodríguez Estefanía , Téllez Mª Mar , Janssen Dirk
Published: July 2019

(1) Background: Tomato leaf curl New Delhi virus (ToLCNDV), transmitted by tobacco whitefly (Bemisia tabaci Gennadius) (Hemiptera: Aleyrodidae), is of major concern in the cultivation of zucchini. The threat of this virus motivates reliance on chemical vector control but European consumers’ demands for vegetables grown free of pesticides provides an important incentive for alternative pest management; (2) Methods: Different whitefly management strategies and ToLCNDV incidences were surveyed in commercial zucchini greenhouses in south-east Spain. In an experimental greenhouse, three different whitefly control strategies, biological, chemical, and integrated (IPM), were evaluated in a replicated trial to determine the most effective strategy for virus suppression (3) Results: Whitefly was present in all commercial zucchini crops surveyed, whereas fewer crops had Amblyseius swirskii or other natural enemies. During three consecutive years, pest management was increasingly based on chemical treatments. Yet, ToLCNDV was widespread in zucchini greenhouses. Experimental results showed that the order of best strategy for virus suppressing was integrated management (73%) > biological control (58%) > chemical control (44%); and (4) Conclusions: IPM was the best strategy for virus suppression. The results can assist in the design of appropriate control strategies for chemical pesticide reduction and decision-making in pest management.
Methanotrophic Bacterial Biomass as Potential Mineral Feed Ingredients for Animals
Authors: Kuźniar Agnieszka , Furtak Karolina , Włodarczyk Kinga , Stępniewska Zofia , Wolińska Agnieszka
Published: July 2019

Microorganisms play an important role in animal nutrition, as they can be used as a source of food or feed. The aim of the study was to determine the nutritional elements and fatty acids contained in the biomass of methanotrophic bacteria. Four bacterial consortia composed of Methylocystis and Methylosinus originating from Sphagnum flexuosum (Sp1), S. magellanicum (Sp2), S. fallax II (Sp3), S. magellanicum IV (Sp4), and one composed of Methylocaldum, Methylosinus, and Methylocystis that originated from coalbed rock (Sk108) were studied. Nutritional elements were determined using the flame atomic absorption spectroscopy technique after a biomass mineralization stage, whereas the fatty acid content was analyzed with the GC technique. Additionally, the growth of biomass and dynamics of methane consumption were monitored. It was found that the methanotrophic biomass contained high concentrations of K, Mg, and Fe, i.e., approx. 9.6–19.1, 2.2–7.6, and 2.4–6.6 g kg−1, respectively. Consequently, the biomass can be viewed as an appropriate feed and/or feed additive for supplementation with macroelements and certain microelements. Moreover, all consortia demonstrated higher content of unsaturated acids than saturated ones. Thus, methanotrophic bacteria seem to be a good solution, in natural supplementation of animal diets.
Personality Traits and Obesity
Authors: Bagnjuk Jelena , König Hans-Helmut , Hajek André
Published: July 2019

Objective: Previous studies investigating the association between body weight and personality traits have found mixed results. This paper uses a large data set and two different study designs (cross-sectional and longitudinal) to provide more consistent estimates of the effect of personality traits on obesity. Methods: The present study is based on data from the German Socio-Economic Panel (GSOEP) from the waves 2005 to 2013; GSOEP is a longitudinal survey of private households in Germany that has been carried out since 1984. Responses provided data about personality, measured using the BFI-S Questionnaire, data for self-rated body mass index BMI (to determine the obesity level), as well as information for potential confounders. Cross-sectional and longitudinal logistic regression models were used. Results: The cross-sectional study yielded statistically significant results for the association between the outcome variable and four personality factors neuroticism, extraversion, openness and agreeableness. After controlling for several potential confounders, the association between obesity and extraversion, openness as well as agreeableness remained; additionally, the personality factor consciousness reached statistical significance. In the longitudinal study, a statistically significant association was found only for two personality factors, namely extraversion and agreeableness. After adding the control variables, the FE-regression yielded an association only for agreeableness (negative). Gender differences were not significant. Discussion: The findings show that results of a cross-sectional study design differ from the outcome of the longitudinal study design. The latter stresses the association between excess weight and the personality factor agreeableness, contrasting with most outcomes of previous research.
Differential Impact of an Education-Based Intervention for Patients with Type 2 Diabetes Mellitus in Rural China
Authors: Chen Shaofan , Burström Bo , Sparring Vibeke , Qian Dongfu , Burström Kristina
Published: July 2019

The study aimed to assess the impact of an education-based intervention to improve vertical integration and management of type 2 diabetes mellitus in primary care in rural China. Patients with type 2 diabetes mellitus in three townships in Jingjiang county, Jiangsu Province were randomly divided into intervention and control groups. Participants in the intervention group received an education-based intervention. Patients’ data including the fasting blood glucose (FBG) level, health-related quality of life (HRQoL), and sociodemographic characteristics were collected at baseline (2015) and follow-up (2016). The FBG levels decreased significantly in the intervention group compared to the control group in the overall analysis. In the stratified analysis, FBG levels and some aspects of HRQoL improved in the intervention group more for females, married persons, those with low education, and those in farming or house working. Participants in the control group deteriorated in FBG levels but improved in some aspects of HRQoL. The intervention improved in FBG levels and some aspects of HRQoL among participants. Furthermore, the intervention seemed to differentially benefit females, married persons, lowly educated persons, and those in farming or house working more than other groups. (Trial registration: ISRCTN, ISRCTN13319989. Retrospectively registered 4 April 2017).
Substantial and Sustained Improvements in Blood Pressure, Weight and Lipid Profiles from a Carbohydrate Restricted Diet: An Observational Study of Insulin Resistant Patients in Primary Care
Authors: Unwin David J. , Tobin Simon D. , Murray Scott W. , Delon Christine , Brady Adrian J.
Published: July 2019

Hypertension is the second biggest known global risk factor for disease after poor diet; perhaps lifestyle interventions are underutilized? In a previous small pilot study, it was found that a low carbohydrate diet was associated with significant improvements in blood pressure, weight, ‘deprescribing’ of medications and lipid profiles. We were interested to investigate if these results would be replicated in a larger study based in ‘real world’ GP practice. 154 patients with type 2 diabetes or impaired glucose tolerance were recruited into an observational cohort study in primary care. The effects of a low carbohydrate diet sustained for an average of two years (interquartile range 10–32 months) on cardiovascular risk factors were examined. Results demonstrate significant and substantial reductions in blood pressure (mean reduction of systolic BP 10.9 mmHg (interquartile range 0–22 mmHg) (p < 0.0001), mean reduction in diastolic BP 6.3 mmHg (interquartile range 0–12.8 mmHg) (p < 0.0001) and mean weight reduction of 9.5 Kg (interquartile range 5–13 Kg) (p < 0.0001) together with marked improvement in lipid profiles. This occurred despite a 20% reduction in anti-hypertensive medications. This novel and potentially highly effective dietary modification, done very cheaply alongside routine care, offers hope that should be tested in a large prospective trial.
Effects of Free-Floating Shared Bicycles on Urban Public Transportation
Authors: Cao Min , Ma Shangjing , Huang Mengxue , Lü Guonian , Chen Min
Published: July 2019

As a product of the development of the Internet and the sharing economy, shared bicycles are beneficial in solving the last mile problem of public transportation for urban residents and expanding the service area of urban public transportation to a certain extent. This paper analyses the spatial-temporal characteristics of shared bicycles in the city of Beijing by using kernel density estimation and statistical analysis methods. The maximum coverage location problem model is used to quantify the effects of shared bicycles on the subway and bus urban public transportation systems. The analysis results are of great importance to identify the effects of shared bicycle usage on an urban bus public transportation system from the view of space-time. The results show that (1) shared bicycles are largely located within 1.5 km from the subway station and 500 m from the bus stations; (2) the number of the shared bicycle usage changes along with the distance from the subway and bus stations; (3) subway and bus stations are divided into six types based on the sink-source characteristics of shared bicycles; (4) the service areas of subway and bus stations are expanded by approximately 2.34 and 1.33 times, respectively, after the implementation of shared bicycles.
Spatial Disaggregation of Historical Census Data Leveraging Multiple Sources of Ancillary Information
Authors: Monteiro João , Martins Bruno , Murrieta-Flores Patricia , Pires João M.
Published: July 2019

High-resolution population grids built from historical census data can ease the analyses of geographical population changes, at the same time also facilitating the combination of population data with other GIS layers to perform analyses on a wide range of topics. This article reports on experiments with a hybrid spatial disaggregation technique that combines the ideas of dasymetric mapping and pycnophylactic interpolation, using modern machine learning methods to combine different types of ancillary variables, in order to disaggregate historical census data into a 200 m resolution grid. We specifically report on experiments related to the disaggregation of historical population counts from three different national censuses which took place around 1900, respectively in Great Britain, Belgium, and the Netherlands. The obtained results indicate that the proposed method is indeed highly accurate, outperforming simpler disaggregation schemes based on mass-preserving areal weighting or pycnophylactic interpolation. The best results were obtained using modern regression methods (i.e., gradient tree boosting or convolutional neural networks, depending on the case study), which previously have only seldom been used for spatial disaggregation.
Querying 3D Cadastral Information from BIM Models
Authors: Atazadeh Behnam , Rajabifard Abbas , Zhang Yibo , Barzegar Maryam
Published: July 2019

There has been significant research on the intersection of 3D cadastre and building information modelling (BIM) over the recent years. BIM provides a multidimensional environment for capturing, curating and communicating the physical and functional aspects during a building’s lifecycle. A BIM-based solution for 3D cadastre provides a rich repository of legal and physical datasets in a common environment. The knowledge encapsulated inside a cadastral BIM model should be tapped to unlock the value of 3D cadastral information. Therefore, this article aims to develop BIM-based queries for interrogating questions about the legal ownership of properties inside multistorey buildings. These queries include identifying legal spaces that belong to a private or common property; querying physical elements that bound a legal space; and finding legal spaces that are adjacent to each other at a specific building element.
Acute Functional Adaptations in Isolated Presynaptic Terminals Unveil Synaptosomal Learning and Memory
Authors: Pittaluga Anna
Published: July 2019

Synaptosomes are used to decipher the mechanisms involved in chemical transmission, since they permit highlighting the mechanisms of transmitter release and confirming whether the activation of presynaptic receptors/enzymes can modulate this event. In the last two decades, important progress in the field came from the observations that synaptosomes retain changes elicited by both “in vivo” and “in vitro” acute chemical stimulation. The novelty of these studies is the finding that these adaptations persist beyond the washout of the triggering drug, emerging subsequently as functional modifications of synaptosomal performances, including release efficiency. These findings support the conclusion that synaptosomes are plastic entities that respond dynamically to ambient stimulation, but also that they “learn and memorize” the functional adaptation triggered by acute exposure to chemical agents. This work aims at reviewing the results so far available concerning this form of synaptosomal learning, also highlighting the role of these acute chemical adaptations in pathological conditions.
Surface-Functionalized Nanoparticles as Efficient Tools in Targeted Therapy of Pregnancy Complications
Authors: Zhang Baozhen , Liang Ruijing , Zheng Mingbin , Cai Lintao , Fan Xiujun
Published: July 2019

Minimizing exposure of the fetus to medication and reducing adverse off-target effects in the mother are the primary challenges in developing novel drugs to treat pregnancy complications. Nanomedicine has introduced opportunities for the development of novel platforms enabling targeted delivery of drugs in pregnancy. This review sets out to discuss the advances and potential of surface-functionalized nanoparticles in the targeted therapy of pregnancy complications. We first describe the human placental anatomy, which is fundamental for developing placenta-targeted therapy, and then we review current knowledge of nanoparticle transplacental transport mechanisms. Meanwhile, recent surface-functionalized nanoparticles for targeting the uterus and placenta are examined. Indeed, surface-functionalized nanoparticles could help prevent transplacental passage and promote placental-specific drug delivery, thereby enhancing efficacy and improving safety. We have achieved promising results in targeting the placenta via placental chondroitin sulfate A (plCSA), which is exclusively expressed in the placenta, using plCSA binding peptide (plCSA-BP)-decorated nanoparticles. Others have also focused on using placenta- and uterus-enriched molecules as targets to deliver therapeutics via surface-functionalized nanoparticles. Additionally, we propose that placenta-specific exosomes and surface-modified exosomes might be potential tools in the targeted therapy of pregnancy complications. Altogether, surface-functionalized nanoparticles have great potential value as clinical tools in the targeted therapy of pregnancy complications.
Roles of MicroRNAs in Establishing and Modulating Stem Cell Potential
Authors: Zhang Zhenwu , Zhuang Lili , Lin Chao-Po
Published: July 2019

Early embryonic development in mammals, from fertilization to implantation, can be viewed as a process in which stem cells alternate between self-renewal and differentiation. During this process, the fates of stem cells in embryos are gradually specified, from the totipotent state, through the segregation of embryonic and extraembryonic lineages, to the molecular and cellular defined progenitors. Most of those stem cells with different potencies in vivo can be propagated in vitro and recapitulate their differentiation abilities. Complex and coordinated regulations, such as epigenetic reprogramming, maternal RNA clearance, transcriptional and translational landscape changes, as well as the signal transduction, are required for the proper development of early embryos. Accumulated studies suggest that Dicer-dependent noncoding RNAs, including microRNAs (miRNAs) and endogenous small-interfering RNAs (endo-siRNAs), are involved in those regulations and therefore modulate biological properties of stem cells in vitro and in vivo. Elucidating roles of these noncoding RNAs will give us a more comprehensive picture of mammalian embryonic development and enable us to modulate stem cell potencies. In this review, we will discuss roles of miRNAs in regulating the maintenance and cell fate potential of stem cells in/from mouse and human early embryos.
Combination with l-Menthol Enhances Transdermal Penetration of Indomethacin Solid Nanoparticles
Authors: Nagai Noriaki , Ogata Fumihiko , Yamaguchi Mizuki , Fukuoka Yuya , Otake Hiroko , Nakazawa Yosuke , Kawasaki Naohito
Published: July 2019

This study designed the transdermal formulations containing indomethacin (IMC)—1% IMC was crushed with 0.5% methylcellulose and 5% 2-hydroxypropyl-β-cyclodextrin by the bead mill method, and the milled IMC was gelled with or without 2% l-menthol (a permeation enhancer) by Carbopol® 934 (without menthol, N-IMC gel; with menthol, N-IMC/MT gel). In addition, the drug release, skin penetration and percutaneous absorption of the N-IMC/MT gel were investigated. The particle sizes of N-IMC gel were approximately 50–200 nm, and the combination with l-menthol did not affect the particle characterization of the transdermal formulations. In an in vitro experiment using a Franz diffusion cell, the skin penetration in N-IMC/MT gel was enhanced than the N-IMC gel, and the percutaneous absorption (AUC) from the N-IMC/MT gel was 2-fold higher than the N-IMC gel. On the other hand, the skin penetration from the N-IMC/MT gel was remarkably attenuated at a 4 °C condition, a temperature that inhibits all energy-dependent endocytosis. In conclusion, this study designed transdermal formulations containing IMC solid nanoparticles and l-menthol, and found that the combination with l-menthol enhanced the skin penetration of the IMC solid nanoparticles. In addition, the energy-dependency of the skin penetration of IMC solid nanoparticles was demonstrated. These findings suggest the utility of a transdermal drug delivery system to provide the easy application of solid nanoparticles (SNPs).
Therapeutic Use of Intranasal Glucagon: Resolution of Hypoglycemia
Authors: Pontiroli Antonio E. , Tagliabue Elena
Published: July 2019

Episodes of hypoglycemia are frequent in patients with diabetes treated with insulin or sulphonylureas. Hypoglycemia can lead to severe acute complications, and, as such, both prevention and treatment of hypoglycemia are important for the well-being of patients with diabetes. The experience of hypoglycemia also leads to fear of hypoglycemia, that in turn can limit optimal glycemic control in patients, especially with type 1 diabetes. Treatment of hypoglycemia is still based on administration of carbohydrates (oral or parenteral according to the level of consciousness) or of glucagon (intramuscular or subcutaneous injection). In 1983, it was shown for the first time that intranasal (IN) glucagon drops (with sodium glycocholate as a promoter) increase blood glucose levels in healthy volunteers. During the following decade, several authors showed the efficacy of IN glucagon (drops, powders, and sprays) to resolve hypoglycemia in normal volunteers and in patients with diabetes, both adults and children. Only in 2010, based on evaluation of patients’ beliefs and patients’ expectations, a canadian pharmaceutical company (Locemia Solutions, Montreal, Canada) reinitiated efforts to develop glucagon for IN administration. The project has been continued by Eli Lilly, that is seeking to obtain registration in order to make IN glucagon available to insulin users (children and adolescents) worldwide. IN glucagon is as effective as injectable glucagon, and devoid of most of the technical difficulties associated with administration of injectable glucagon. IN glucagon appears to represent a major breakthrough in the treatment of severe hypoglycemia in insulin-treated patients with diabetes, both children and adults.