bluuebunny/crossref_metadata_2025 huggingface dataset:
DeepResTrade: a peer-to-peer LSTM-decision tree-based price prediction and blockchain-enhanced trading system for renewable energy decentralized markets
Authors: Safari Ashkan , Gharehbagh Hamed Kheirandish , Nazari-Heris Morteza , Oshnoei Arman
Published: September 2023

Intelligent predictive models are fundamental in peer-to-peer (P2P) energy trading as they properly estimate supply and demand variations and optimize energy distribution, and the other featured values, for participants in decentralized energy marketplaces. Consequently, DeepResTrade is a research work that presents an advanced model for predicting prices in a given traditional energy market. This model includes numerous fundamental components, including the concept of P2P trading systems, long-term and short-term memory (LSTM) networks, decision trees (DT), and Blockchain. DeepResTrade utilized a dataset with 70,084 data points, which included maximum/minimum capacities, as well as renewable generation, and price utilized of the communities. The developed model obtains a significant predictive performance of 0.000636% Mean Absolute Percentage Error (MAPE) and 0.000975% Root Mean Square Percentage Error (RMSPE). DeepResTrade’s performance is demonstrated by its RMSE of 0.016079 and MAE of 0.009125, indicating its capacity to reduce the difference between anticipated and actual prices. The model performs admirably in describing actual price variations in, as shown by a considerable R2 score of 0.999998. Furthermore, F1/recall scores of [1, 1, 1] with a precision of 1, all imply its accuracy.
Lung inflammation and interstitial fibrosis by targeted alveolar epithelial type I cell death
Authors: Carignon Sandra , De Moura Rodrigues Dorian , Gosset David , Culerier Elodie , Huot-Marchand Sarah , Savigny Florence , Kaya Eric , Quesniaux Valerie , Gombault Aurélie , Couillin Isabelle , Ryffel Bernhard , Le Bert Marc , Riteau Nicolas
Published: September 2023

Introduction

The pathogenesis of chronic lung diseases is multifaceted with a major role of recurrent micro-injuries of the epithelium. While several reports clearly indicated a prominent role for surfactant-producing alveolar epithelial type 2 (AT2) cells, the contribution of gas exchange-permissive alveolar epithelial type 1 (AT1) cells has not been addressed yet. Here, we investigated whether repeated injury of AT1 cells leads to inflammation and interstitial fibrosis.

Methods

We chose an inducible model of AT1 cell depletion following local diphtheria toxin (DT) administration using an iDTR flox/flox (idTRfl/fl) X Aquaporin 5CRE (Aqp5CRE) transgenic mouse strain.

Results

We investigated repeated doses and intervals of DT to induce cell death of AT1 cells causing inflammation and interstitial fibrosis. We found that repeated DT administrations at 1ng in iDTRfl/fl X Aqp5CRE mice cause AT1 cell death leading to inflammation, increased tissue repair markers and interstitial pulmonary fibrosis.

Discussion

Together, we demonstrate that depletion of AT1 cells using repeated injury represents a novel approach to investigate chronic lung inflammatory diseases and to identify new therapeutic targets.
Stroke-induced damage on the blood–brain barrier
Authors: Xue Song , Zhou Xin , Yang Zhi-Hui , Si Xiang-Kun , Sun Xin
Published: September 2023

The blood–brain barrier (BBB) is a functional phenotype exhibited by the neurovascular unit (NVU). It is maintained and regulated by the interaction between cellular and non-cellular matrix components of the NVU. The BBB plays a vital role in maintaining the dynamic stability of the intracerebral microenvironment as a barrier layer at the critical interface between the blood and neural tissues. The large contact area (approximately 20 m2/1.3 kg brain) and short diffusion distance between neurons and capillaries allow endothelial cells to dominate the regulatory role. The NVU is a structural component of the BBB. Individual cells and components of the NVU work together to maintain BBB stability. One of the hallmarks of acute ischemic stroke is the disruption of the BBB, including impaired function of the tight junction and other molecules, as well as increased BBB permeability, leading to brain edema and a range of clinical symptoms. This review summarizes the cellular composition of the BBB and describes the protein composition of the barrier functional junction complex and the mechanisms regulating acute ischemic stroke-induced BBB disruption.
AutoML accurately predicts endovascular mechanical thrombectomy in acute large vessel ischemic stroke
Authors: Raj Rishi , Kannath Santhosh Kumar , Mathew Jimson , Sylaja P. N.
Published: September 2023

Background and objective

Automated machine learning or autoML has been widely deployed in various industries. However, their adoption in healthcare, especially in clinical settings is constrained due to a lack of clear understanding and explainability. The aim of this study is to utilize autoML for the prediction of functional outcomes in patients who underwent mechanical thrombectomy and compare it with traditional ML models with a focus on the explainability of the trained models.

Methods

A total of 156 patients of acute ischemic stroke with Large Vessel Occlusion (LVO) who underwent mechanical thrombectomy within 24 h of stroke onset were included in the study. A total of 34 treatment variables including clinical, demographic, imaging, and procedure-related data were extracted. Various conventional machine learning models such as decision tree classifier, logistic regression, random forest, kNN, and SVM as well as various autoML models such as AutoGluon, MLJAR, Auto-Sklearn, TPOT, and H2O were used to predict the modified Rankin score (mRS) at the time of patient discharge and 3 months follow-up. The sensitivity, specificity, accuracy, and AUC for traditional ML and autoML models were compared.

Results

The autoML models outperformed the traditional ML models. For the prediction of mRS at discharge, the highest testing accuracy obtained by traditional ML models for the decision tree classifier was 74.11%, whereas for autoML which was obtained through AutoGluon, it showed an accuracy of 88.23%. Similarly, for mRS at 3 months, the highest testing accuracy of traditional ML was that of the SVM classifier at 76.5%, whereas that of autoML was 85.18% obtained through MLJAR. The 24-h ASPECTS score was the most important predictor for mRS at discharge whereas for prediction of mRS at 3 months, the most important factor was mRS at discharge.

Conclusion

Automated machine learning models based on multiple treatment variables can predict the functional outcome in patients more accurately than traditional ML models. The ease of clinical coding and deployment can assist clinicians in the critical decision-making process. We have developed a demo application which can be accessed at https://mrs-score-calculator.onrender.com/.
Analysis of public food procurement in relation to dairy products and their quality criteria
Authors: Brukało Katarzyna M. , Nowak Justyna , Fras Neza , Kowalski Oskar , Gabrijelčič Blenkuš Mojca
Published: September 2023

Introduction

Public procurement of food is key to ensuring proper nutrition with high-quality products in public institutions such as schools and kindergartens. However, it should not be considered a mandatory expenditure from public finances but rather an investment in health promotion.

Materials and methods

A total of 1,126 public procurement orders processed by schools and kindergartens in Poland during the period from November 2022 to March 2023 were analyzed. Ultimately, 197 public procurement orders meeting the inclusion criteria were considered for analysis. Based on these orders, 2,753 food products classified as dairy and its derivatives were extracted. The ordered quantities of individual products were analyzed, as well as their descriptions (quality characteristics).

Results

Criteria related to composition were most commonly described, the most common criterion was the fat content and the absence of preservatives. On the second places were organoleptic characteristics, where taste and consistency expectations were most frequently specified. Sustainable public procurement criteria were the least frequently mentioned and were treated as highly marginal.

Conclusion

Introducing minimum standards for the descriptions of dairy products in terms of organoleptic characteristics, composition features, and sustainability criteria will improve the quality of dairy products supplied to public institutions, particularly schools and kindergartens.
Leukemic conversion involving RAS mutations of type 1 CALR-mutated primary myelofibrosis in a patient treated for HCV cirrhosis: a case report
Authors: Gurban Petruta , Mambet Cristina , Botezatu Anca , Necula Laura G. , Neagu Ana I. , Matei Lilia , Pitica Ioana M. , Nedeianu Saviana , Chivu-Economescu Mihaela , Bleotu Coralia , Ataman Marius , Mocanu Gabriela , Saguna Carmen , Pavel Anca G. , Stambouli Danae , Sepulchre Elise , Anton Gabriela , Diaconu Carmen C. , Constantinescu Stefan N.
Published: September 2023

Somatic frameshift mutations in exon 9 of calreticulin (CALR) gene are recognized as disease drivers in primary myelofibrosis (PMF), one of the three classical Philadelphia-negative myeloproliferative neoplasms (MPNs). Type 1/type 1-like CALR mutations particularly confer a favorable prognostic and survival advantage in PMF patients. We report an unusual case of PMF incidentally diagnosed in a 68-year-old woman known with hepatitis C virus (HCV) cirrhosis who developed a progressive painful splenomegaly, without anomalies in blood cell counts. While harboring a type 1 CALR mutation, the patient underwent a leukemic transformation in less than 1 year from diagnosis, with a lethal outcome. Analysis of paired DNA samples from chronic and leukemic phases by a targeted next-generation sequencing (NGS) panel and single-nucleotide polymorphism (SNP) microarray revealed that the leukemic clone developed from the CALR-mutated clone through the acquisition of genetic events in the RAS signaling pathway: an increased variant allele frequency of the germline NRAS Y64D mutation present in the chronic phase (via an acquired uniparental disomy of chromosome 1) and gaining NRAS G12D in the blast phase. SNP microarray analysis showed five clinically significant copy number losses at regions 7q22.1, 8q11.1-q11.21, 10p12.1-p11.22, 11p14.1-p11.2, and Xp11.4, revealing a complex karyotype already in the chronic phase. We discuss how additional mutations, detected by NGS, as well as HCV infection and antiviral therapy, might have negatively impacted this type 1 CALR-mutated PMF. We suggest that larger studies are required to determine if more careful monitoring would be needed in MPN patients also carrying HCV and receiving anti-HCV treatment.
Anti-oxidant effects of cannabidiol relevant to intracerebral hemorrhage
Authors: Yan Gaili , Zhang Xiangyu , Li Hongmin , Guo Yan , Yong V. Wee , Xue Mengzhou
Published: September 2023

Intracerebral hemorrhage (ICH) is a subtype of stroke with a high mortality rate. Oxidative stress cascades play an important role in brain injury after ICH. Cannabidiol, a major non-psychotropic phytocannabinoids, has drawn increasing interest in recent years as a potential therapeutic intervention for various neuropsychiatric disorders. Here we provide a comprehensive review of the potential therapeutic effects of cannabidiol in countering oxidative stress resulting from ICH. The review elaborates on the various sources of oxidative stress post-ICH, including mitochondrial dysfunction, excitotoxicity, iron toxicity, inflammation, and also highlights cannabidiol’s ability to inhibit ROS/RNS generation from these sources. The article also delves into cannabidiol’s role in promoting ROS/RNS scavenging through the Nrf2/ARE pathway, detailing both extranuclear and intranuclear regulatory mechanisms. Overall, the review underscores cannabidiol’s promising antioxidant effects in the context of ICH and suggests its potential as a therapeutic option.
Nuciferine induces autophagy to relieve vascular cell adhesion molecule 1 activation via repressing the Akt/mTOR/AP1 signal pathway in the vascular endothelium
Authors: Wei Haibin , Yin Yujie , Yang Wenwen , Zhu Jinyan , Chen Lin , Guo Rui , Yang Zhen , Li Songtao
Published: September 2023

Pro-inflammatory factor-associated vascular cell adhesion molecule 1 (VCAM1) activation initiates cardiovascular events. This study aimed to explore the protective role of nuciferine on TNFα-induced VCAM1 activation. Nuciferine was administrated to both high-fat diet (HFD)-fed mice and the TNFα-exposed human vascular endothelial cell line. VCAM1 expression and further potential mechanism(s) were explored. Our data revealed that nuciferine intervention alleviated VCAM1 activation in response to both high-fat diet and TNFα exposure, and this protective effect was closely associated with autophagy activation since inhibiting autophagy by either genetic or pharmaceutical approaches blocked the beneficial role of nuciferine. Mechanistical studies revealed that Akt/mTOR inhibition, rather than AMPK, SIRT1, and p38 signal pathways, contributed to nuciferine-activated autophagy, which further ameliorated TNFα-induced VCAM1 via repressing AP1 activation, independent of transcriptional regulation by IRF1, p65, SP1, and GATA6. Collectively, our data uncovered a novel biological function for nuciferine in protecting VCAM1 activation, implying its potential application in improving cardiovascular events.
Effects of high-definition transcranial direct current stimulation on the cortical−muscular functional coupling and muscular activities of ankle dorsi−plantarflexion under running-induced fatigue
Authors: Zhan Jianglong , Yu Changxiao , Xiao Songlin , Shen Bin , Zhang Chuyi , Zhou Junhong , Fu Weijie
Published: September 2023

Transcranial direct current stimulation (tDCS) can improve motor control performance under fatigue. However, the influences of tDCS on factors contributing to motor control (e.g., cortical−muscular functional coupling, CMFC) are unclear. This double-blinded and randomized study examined the effects of high-definition tDCS (HD-tDCS) on muscular activities of dorsiflexors and plantarflexors and CMFC when performing ankle dorsi–plantarflexion under fatigue. Twenty-four male adults were randomly assigned to receive five sessions of 20-min HD-tDCS targeting primary motor cortex (M1) or sham stimulation. Three days before and 1 day after the intervention, participants completed ankle dorsi–plantarflexion under fatigue induced by prolonged running exercise. During the task, electroencephalography (EEG) of M1 (e.g., C1, Cz) and surface electromyography (sEMG) of several muscles (e.g., tibialis anterior [TA]) were recorded synchronously. The corticomuscular coherence (CMC), root mean square (RMS) of sEMG, blood lactate, and maximal voluntary isometric contraction (MVC) of ankle dorsiflexors and plantarflexors were obtained. Before stimulation, greater beta- and gamma-band CMC between M1 and TA were significantly associated with greater RMS of TA (r = 0.460–0.619, p = 0.001–0.024). The beta- and gamma-band CMC of C1-TA and Cz-TA, and RMS of TA and MVC torque of dorsiflexors were significantly higher after HD-tDCS than those at pre-intervention in the HD-tDCS group and post-intervention in the control group (p = 0.002–0.046). However, the HD-tDCS-induced changes in CMC and muscle activities were not significantly associated (r = 0.050–0.128, p = 0.693–0.878). HD-tDCS applied over M1 can enhance the muscular activities of ankle dorsiflexion under fatigue and related CMFC.
Being more satisfied with romantic relationship status is associated with increased mental wellbeing in people with experience of psychosis
Authors: White Rebecca , Haddock Gillian , Haarmans Maria , Varese Filippo
Published: September 2023

Aims

Romantic relationships represent one of the most salient sources of social support. In general population studies, they are associated with both physical and psychological benefits. Research suggests that for people with psychosis, romantic relationships may also have a positive impact on a range of outcomes, but the reasons for these associations are still unclear. This study aims to investigate whether satisfaction with romantic relationships status is associated with better wellbeing outcomes in people with experience of psychosis and explore three possible psychological mediators of this relationship.

Methods

Participants who had previously sought support for psychosis (n = 190) completed an online survey including measures of relationship status satisfaction (the Satisfaction with Relationship Scale) as well as measures of psychotic symptoms (the CAPE-42), general well-being (Short Warwick-Edinburgh Mental Wellbeing Scale) and several psychological variables relevant to the pathway between romantic relationships and well-being outcomes, namely loneliness, internalised stigma, self-esteem and attachment.

Results

Fearful attachment and partner criticism were negatively associated with relationship status satisfaction. Having a partner was positively associated with relationship status satisfaction. Higher levels of relationship status satisfaction were associated with lower psychotic symptoms and higher mental wellbeing. This relationship was mediated by loneliness, internalised stigma, and self-esteem.

Conclusion

Mental health services should be mindful of the associations between romantic relationship satisfaction and wellbeing. Service users with a fearful attachment style may particularly benefit from support in this area.
Do media coverage of suicides and search frequency on suicides predict the number of tweets seeking others for a suicide pact?
Authors: Lee Sang Yup
Published: September 2023

We examined whether media coverage of suicides and frequencies of searching for suicide methods or suicide pacts predicted the number of users posting tweets seeking others for a suicide pact. Analyses of 6,119 tweets containing “suicide pact” posted on Twitter during a 6-month period revealed that the number of users posting tweets seeking others for a suicide pact had a positive association with media coverage of celebrity suicides, but not with that of suicide pact victims, and a greater positive association with the search frequency for suicide methods than for suicide pacts. We found that the search frequency on suicide methods was positively associated with media coverage of celebrity suicides, while that on suicide pacts was more strongly related to media coverage of suicide pacts.
Are wheat-based farming systems in South Asia feminizing?
Authors: Farnworth Cathy Rozel , Gartaula Hom N. , Badstue Lone B. , Roeven Lara , Bharati Preeti , Rahman Siddiqur , Petesch Patti , Jafry Tahseen
Published: September 2023

This article pulls together the state of knowledge on the degree to which wheat-based systems in Bangladesh, India, Nepal, and Pakistan, are feminizing. It is not yet possible to make definitive statements. However, it is clear that wheat-based systems are undergoing far-reaching changes in relation to “who does what” and “who decides.” There are some commonalities across all four countries. Intersectionalities shape women’s identities and abilities to exert their agency. Purdah is a cultural norm in many locations. Nevertheless, each country displays different meta-trends. In Nepal managerial feminization is increasing unlike in Pakistan. Women in Bangladesh spend the least time in field work whereas in other countries they are often strongly engaged. There are strong local variations within countries as well which we explore. Establishing the extent of feminization is challenging because studies ask different questions, operate at different levels, and are rarely longitudinal. Researchers often construct men as primary farmers, leading to a failure to find out what men and women really do and decide. This diminishes the value of many studies. Cultural perceptions of honor can make men respondents reluctant to report on women’s agency and women can be reluctant to claim agency openly. We provide suggestions for better research, and urge support to women as workers and decision-makers.
Improving productive performance, immunity, and health status of growing rabbits by using honey bee venom (Apis mellifera)
Authors: Elkomy Alaa E. , Sadaka Tarek A. , Hassan Saber S. , Shawky Omnia , El-Speiy Mohamed E. , El-Beshkar Mohamed , Wadaan Mohammad A. M. , El-Tahan Hatem M. , Cho Sungbo , Kim In Ho , El-Tahan Hossam M.
Published: September 2023

To investigate the effect of bee venom (BV) as a natural growth promotor on growing rabbits as an alternative to antibiotics, sixty 35-day-old Californian male rabbits with an average body weight of 584 ± 9 gm were randomly divided into five equal groups as follows: The 2nd group received drinking water supplied with 10 mg Oxytetracycline (OXT), while the 3rd, 4th, and 5th groups received 2, 4 and 8 mg bee venom (BV)/kg body weight/day in drinking water, and the first group was served as a control group. The growth performance features were positively impacted by adding BV (p ≤ 0.01) compared to the control, whereas LBW and BWG increased and FI reduced. Significantly improved carcass characteristics (p ≤ 0.01) as a result of the BV supplementation. Blood characteristics showed a significant reduction (p ≤ 0.01) in liver enzyme activities and Cholesterol, Triglycerides, and Low-density lipoproteins Cholesterol (LDL) as affected by BV treatment; inversely, total protein and globulin were significantly increased (p ≤ 0.01). Similarly, BV had a positive effect (p ≤ 0.01) on anti-oxidant status (Total anti-oxidant capacity (TAC), Glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT)). In contrast, the lipid peroxidation biomarker (Malondialdehyde (MDA)) was significantly decreased. The immunoglobulin (IgG and IgM) was significantly increased (p ≤ 0.01) by BV treatment. There was a positive effect of low BV levels on decreasing both cecum TBC and pathogenic bacterial count (Salmonella spp., E.coli spp., Proteus spp., and Clostridia spp.) that was significant (p ≤ 0.01). In conclusion, BV can be a natural growth promoter to enhance growth performance traits, immunological and anti-oxidative responses, and reduce pathogenic bacteria in the hindgut of growing rabbits.
The Relationship between Dietary Intake and Adiposity in South African Female Adolescents: A Systematic Review
Authors: Vilakazi Nokuthula , Mathunjwa Sithabile , Legodi Heather , Pisa Pedro Terrence
Published: September 2023

The prevalence of obesity has increased significantly in developing nations over the past decade, particularly among adolescent girls. To assess the scale of this epidemic among female adolescents in South Africa, a systematic review was undertaken to investigate the connection between diet and obesity. Multiple databases (Google Scholar, Science Direct, Cochrane Library, PubMed, and Web of Science) were searched to identify studies investigating the associations between diet and various adiposity indices as outcomes. Of the 56 studies identified, 7 met the inclusion criteria. The age range of participants spanned from 11 to 21 years. Tabulation was used to report the data, study by study. The consumption of nutrients from animal sources exhibited a positive correlation with higher BMI-for-age Z scores (p = 0.02). Eating habits such as sporadic family meals (p ≤ 0.02), irregular breakfast consumption (p ≤ 0.05), and a high energy intake derived from fat were linked to an increased risk of adiposity. Additionally, factors such as socioeconomic status and residential location revealed associations with certain dietary intakes and adiposity. As more studies identify the causative role of diet in obesity, there is an urgent need for policy intervention and strategies to address the growing non-communicable disease burden in South Africa.
The Impact of Surface Waves and Spray Injection Velocities on Air–Sea Momentum and Heat Fluxes
Authors: Zhang Ting
Published: September 2023

Surface waves and sea spray play a significant role in air–sea fluxes in high winds. The present study used a marine atmosphere surface layer (MASL), which couples the traditional Monin–Obukhov similarity theory, sea spray generation function, the balance of turbulent kinetic budget, and momentum/enthalpy conservation equations. Based on this model, the effects of wave states and spray injection velocities on air–sea momentum/enthalpy fluxes and near-surface wind/temperature profiles were theoretically investigated. Based on the assumption that the velocity of injected spray is the same as that of the ambient airflow, it was found that spray could increase the near-surface air turbulence intensity and inhibit air–sea fluxes at 10 m above the sea surface. Correspondingly, near-surface wind speeds and temperature increase in high winds. This phenomenon becomes prominent in cases of large wave ages or surface waves supporting a minority of air–sea fluxes. Based on the assumption that the velocity of the edges of breaking water bags is used to estimate that of spray injection, the opposite results were found: spray could weaken the near-surface air turbulence and increase total air–sea fluxes at 10 m above the sea surface. In this case, the near-surface wind speeds and temperature decreased. This reduction becomes remarkable when surface waves are full-developed or the majority of air–sea momentum fluxes are supported by waves.
The Difference in Cloud Water Resources and Precipitation on the Eastern and Western Sides of the Liupan Mountains Caused by Topographic Effects
Authors: Xu Rui , Qiu Yujun
Published: September 2023

In order to explore the possible impact of topography on precipitation between the eastern and western sides of the Liupan Mountains (LMs) in the northwest region of China, the differences in distribution characteristics of total column water vapor (TCWV), total column cloud water (TCCW), and total precipitation (TP) were studied by using the 40-year hourly data of the fifth generation reanalysis (ERA5) from the European Centre for Medium-Range Weather Forecasts (ECMWF). The results showed the following: (1) The TCCW and TP on the eastern and western sides of the LMs decrease gradually from south to north, following a southwestward bias along the latitude. The high values of the TCCW and TP are predominantly concentrated in the period from July to September. The greatest difference between the two sides occurs in September, with the eastern side exhibiting 15% and 18% higher values compared to the western side, respectively. (2) Both the TCCW and TP exhibit distinct diurnal distribution patterns. The high values on the eastern side persist for a longer duration throughout the day compared to the western side, and they occur in more consecutive months. There is a certain correlation between the steepness of the eastern slope and the gentle gradient of the western slope. Additionally, the occurrence of these high values in the afternoon is earlier on the eastern side compared to the western side. (3) The monthly mean TP is significantly linearly correlated with the TCWV and TCCW, with slightly higher coefficients for the western side compared to the eastern side. This relationship is closely related to the topography of the mountain range. The regression equation provides a quantitative tool for predicting the monthly mean TP in the LM region and serves as a reference basis for the development of cloud water resources in the area.
Characteristics of the Blitzortung.org Lightning Location Catalog in Japan
Authors: Kamogawa Masashi , Suzuki Tomoyuki , Fujiwara Hironobu , Narita Tomomi , Wanke Egon , Murata Kotaro , Nagao Toshiyasu , Kodama Tetsuya , Izutsu Jun , Matsuki Atsushi , Tang Ning , Minamoto Yasuhiro
Published: September 2023

We evaluated the detection efficiency and location accuracy of lightning discharges in Japan using Blitzortung.org, a volunteer-based network for locating lightning discharges from sferics measured by very low frequency (VLF) electromagnetic receivers that have been deployed worldwide in recent years. A comparison of the flash rate (the detected lightning rate per area and period) from Blitzortung.org with that from the satellite-based OTD/LIS and the ground-based World Wide Lightning Location Network (WWLLN) observations showed that Blitzortung.org clearly observed intense lightning activity in and around the Kanto area, including Tokyo, in summer, which is typical of Japanese lightning activity. However, it did not clearly observe lightning activity in and around the Nansei Islands, including Okinawa. Conversely, Blitzortung.org observed winter lightning activity in the Hokuriku area and off the Kanto. In addition, event studies have compared the detection efficiency and location accuracy of Blitzortung.org with those of the Japanese Lightning Location Network (JLDN) to infer their absolute values. The latest detection efficiency of Blitzortung.org in the Kanto area was estimated at roughly 90%. The mean location accuracy was estimated at up to 5.6 km.
Cognitive Crescendo: How Music Shapes the Brain’s Structure and Function
Authors: Toader Corneliu , Tataru Calin Petru , Florian Ioan-Alexandru , Covache-Busuioc Razvan-Adrian , Bratu Bogdan-Gabriel , Glavan Luca Andrei , Bordeianu Andrei , Dumitrascu David-Ioan , Ciurea Alexandru Vlad
Published: September 2023

Music is a complex phenomenon with multiple brain areas and neural connections being implicated. Centuries ago, music was discovered as an efficient modality for psychological status enrichment and even for the treatment of multiple pathologies. Modern research investigations give a new avenue for music perception and the understanding of the underlying neurological mechanisms, using neuroimaging, especially magnetic resonance imaging. Multiple brain areas were depicted in the last decades as being of high value for music processing, and further analyses in the neuropsychology field uncover the implications in emotional and cognitive activities. Music listening improves cognitive functions such as memory, attention span, and behavioral augmentation. In rehabilitation, music-based therapies have a high rate of success for the treatment of depression and anxiety and even in neurological disorders such as regaining the body integrity after a stroke episode. Our review focused on the neurological and psychological implications of music, as well as presenting the significant clinical relevance of therapies using music.
Comparative Analysis of the 2023 Pazarcık and Elbistan Earthquakes in Diyarbakır
Authors: Karasin Ibrahim Baran
Published: September 2023

Türkiye is prone to earthquakes due to its location on various tectonic plates, which can lead to a loss of lives and property. Recently, on 6 February 2023, two major earthquakes hit Pazarcık and Elbistan in Türkiye, causing widespread destruction on the East Anatolian Fault (EAF) zone. Even Diyarbakır, a distant province from the epicentre, was severely affected, highlighting the need to evaluate Turkish earthquake codes. As part of this evaluation, a structural analysis was conducted on earthquake-damaged and collapsed buildings in Diyarbakır. The study analysed three buildings with different levels of damage and six collapsed buildings as case studies. The seismic parameters of the earthquakes were compared to the values in the two recent earthquake hazard maps used in Türkiye’s codes, as well as the Eurocode 8 damage limit values obtained from pushover analysis. The results revealed significant differences between the current seismic values of earthquakes and the current peak ground acceleration (PGA) values specified in the Turkish Earthquake Design Regulations. Additionally, the selected buildings showed inadequate structural behaviours, with significant differences between the expected and actual seismic performances with respect to the PGA values as one of the most important earthquake characteristics.
Colorectal Cancer Fast Tracks: Cancer Yield and the Predictive Value of Entry Criteria
Authors: Uebel Linnea , Kromodikoro Indy , Nyhlin Nils , van Nieuwenhoven Michiel
Published: September 2023

Background: Fast-track pathways for diagnosing colorectal cancer (CRC) have been implemented in several European countries. In Sweden, a substantial number of CRC are diagnosed via the Swedish Standardized Course of Care for colorectal cancer (SCC-CRC). We evaluated the SCC-CRC in terms of CRC yield, and predictive values and odds ratios (OR) for the entry criteria. Methods: We retrospectively analyzed all 2539 patients referred for SCC-CRC colonoscopy between September 2016 and December 2020. Entry criteria and colonoscopy outcomes were analyzed. Results: CRC yield was 16.4%. Highest positive predictive values (PPVs) were seen for abnormal radiology (PPV 30.5%, OR 4.7 (95% CI 3.4–6.4) p < 0.001), abnormal rectal examination (PPV 28%, OR 3.6 (95% CI 2.7–4.8) p < 0.001), and anemia (PPV 24.8%, OR 2.2 (95% CI 1.5–3.1) p < 0.001). Some entry criteria showed no significant risk increase, i.e., visible blood in stool/rectal bleeding, change in bowel habits, and the combination of changed bowel habits plus anemia. A positive fecal immunochemical test (FIT), although not part of the SCC-CRC, showed the highest OR: 9.9 (95% CI 4.5–21.7) p < 0.001) and PPV of 18.8%. Conclusions: CRC yield from the SCC-CRC is slightly higher compared to other European fast tracks. A number of entry criteria showed no benefit towards assessing CRC risk. FIT testing should be included in CRC fast tracks to increase diagnostic efficacy.
WNT4 Gene and Protein Expression in Endometrial Cancer and Its Significance
Authors: Kiewisz Jolanta , Waśniewski Tomasz , Kieżun Jacek , Skowrońska Agnieszka , Kaczmarek Monika M. , Szóstak Błażej , Kowalczyk Anna E. , Kmieć Zbigniew
Published: September 2023

Background: The inappropriate action of WNT4 and estrogens affects uterine homeostasis and function, and may lead to endometrial cancer (EC). Objective: The aim was to evaluate the alterations of WNT4 gene expression and WNT4 protein immunoreactivity (Ir) in EC, considering tumor characteristics, the clinicopathological association and estrogen dependence. Methods: WNT4 mRNA levels were compared between benign (control) endometrium (n = 8) and endometroid EC (EEC) and non-endometroid EC (non-EEC) samples (n = 28) using the real-time PCR technique. The WNT4-Ir and ERα-Ir were evaluated by immunohistochemistry (IHC). WNT4 mRNA gene and WNT4-Ir were correlated with clinicopathological and blood morphological parameters. Overall survival (OS) was assessed. The bioanalysis was utilized to study WNT4 expression in large patient cohort (n = 549). Results: WNT4 gene expression was decreased in EC samples (specifically in EEC but not in non-EEC) compared to the control. The WNT4 gene expression was also decreased in EC samples categorized by the tumor characteristics. There was no statistical difference in WNT4-Ir or ERα-Ir between the control and EC. There was no correlation between OS and WNT4 gene expression and WNT4-Ir. Bioanalysis showed that WNT4 and ESR1 gene expression alterations tended to be mutually exclusive. An alteration in WNT4 expression was found in different histological tumor types in a large group of EC patients. Conclusions: There is a great need to evaluate the molecular background of EC. Our study suggests that the WNT4 gene has the potential to be a marker of functional estrogen signaling in EEC.
Geriatric Oncology as an Unmet Workforce Training Need in the United Kingdom—A Narrative Review by the British Oncology Network for Undergraduate Societies (BONUS) and the International Society of Geriatric Oncology (SIOG) UK Country Group
Authors: Khoury Emma G. , Nuamek Thitikorn , Heritage Sophie , Fulton-Ward Taylor , Kucharczak Joanna , Ng Cassandra , Kalsi Tania , Gomes Fabio , Lind Michael J. , Battisti Nicolò M. L. , Cheung Kwok-Leung , Parks Ruth , Pearce Jessica , Baxter Mark A.
Published: September 2023

Cancer is a disease associated with ageing. Managing cancer in older adults may prove challenging owing to pre-existing frailty, comorbidity, and wider holistic needs, as well as the unclear benefits and harms of standard treatment options. With the ongoing advances in oncology and the increasing complexity of treating older adults with cancer, the geriatric oncology field must be a priority for healthcare systems in education, research, and clinical practice. However, geriatric oncology is currently not formally taught in undergraduate education or postgraduate training programmes in the United Kingdom (UK). In this commentary, we outline the landscape of geriatric oncology undergraduate education and postgraduate training for UK doctors. We highlight current challenges and opportunities and provide practical recommendations for better preparing the medical workforce to meet the needs of the growing population of older adults with cancer. This includes key outcomes to be considered for inclusion within undergraduate and postgraduate curricula.
Alteration of the Exhaled Volatile Organic Compound Pattern in Colorectal Cancer Patients after Intentional Curative Surgery—A Prospective Pilot Study
Authors: Hanevelt Julia , Schoenaker Ivonne J. H. , Brohet Richard M. , Schrauwen Ruud W. M. , Baas Frederique J. N. , Tanis Pieter J. , van Westreenen Henderik L. , de Vos tot Nederveen Cappel Wouter H.
Published: September 2023

As current follow-up modalities for colorectal carcinoma (CRC) have restricted sensitivity, novel diagnostic tools are needed. The presence of CRC changes the endogenous metabolism, resulting in the release of a specific volatile organic compounds (VOC) pattern that can be detected with an electronic nose or AeonoseTM. To evaluate the use of an electronic nose in the follow-up of CRC, we studied the effect of curative surgery on the VOC pattern recognition using AeonoseTM. A prospective cohort study was performed, in which 47 patients diagnosed with CRC were included, all of whom underwent curative surgical resection. Breath testing was performed before and after surgery using the AeonoseTM. A machine learning model was developed by discerning between the 94 pre-and postoperative breath samples. The training model differentiated between the pre-and postoperative CRC breath samples with a sensitivity and specificity of 0.78 (95%CI 0.61–0.90) and 0.73 (95%CI 0.56–0.86), respectively, with an accuracy of 0.76 (95%CI 0.66–0.85), and an area under the curve of 0.79 (95%CI 0.68–0.89). The internal validation of the test set resulted in an accuracy of 0.75 (95%CI 0.51–0.91) and AUC of 0.82 (95%CI 0.61–1). In conclusion, our results suggest that the VOC pattern of CRC patients is altered by curative surgery in a short period, indicating that the exhaled VOCs might be closely related to the presence of CRC. However, to use AeonoseTM as a potential diagnostic tool in the clinical follow-up of CRC patients, the performance of the models needs to be improved through further large-scale prospective research.
Advancing Understanding of Non-Small Cell Lung Cancer with Multiplexed Antibody-Based Spatial Imaging Technologies
Authors: Gray Simon , Ottensmeier Christian H.
Published: September 2023

Non-small cell lung cancer (NSCLC) remains a cause of significant morbidity and mortality, despite significant advances made in its treatment using immune checkpoint inhibitors (ICIs) over the last decade; while a minority experience prolonged responses with ICIs, benefit is limited for most patients. The development of multiplexed antibody-based (MAB) spatial tissue imaging technologies has revolutionised analysis of the tumour microenvironment (TME), enabling identification of a wide range of cell types and subtypes, and analysis of the spatial relationships and interactions between them. Such study has the potential to translate into a greater understanding of treatment susceptibility and resistance, factors influencing prognosis and recurrence risk, and identification of novel therapeutic approaches and rational treatment combinations to improve patient outcomes in the clinic. Herein we review studies that have leveraged MAB technologies to deliver novel insights into the TME of NSCLC.
Activity in Group-Housed Home Cages of Mice as a Novel Preclinical Biomarker in Oncology Studies
Authors: Terry Stéphane , Gommet Céline , Kerangueven Anne-Cécile , Leguet Mickaël , Thévenin Vincent , Berthelot Mickaël , Begoud Laurent , Windenberger Fanny , Lainee Pierre
Published: September 2023

Background: Improving experimental conditions in preclinical animal research is a major challenge, both scientifically and ethically. Automated digital ventilated cages (DVC®) offer the advantage of continuous monitoring of animal activity in their home-cage. The potential utility of this technology remains understudied and deserves investigation in the field of oncology. Methods: Using the DVC® platform, we sought to determine if the continuous assessment of locomotor activity of mice in their home cages can serve as useful digital readout in the monitoring of animals treated with the reference oncology compounds cisplatin and cyclophosphamide. SCID mice of 14 weeks of age were housed in DVC® cages in groups of four and followed with standard and digital examination before and after treatment over a 17-day total period. Results: DVC® detected statistically significant effects of cisplatin on the activity of mice in the short and long term, as well as trends for cyclophosphamide. The activity differences between the vehicle- and chemotherapy-treated groups were especially marked during the nighttime, a period when animals are most active and staff are generally not available for regular checks. Standard clinical parameters, such as body weight change and clinical assessment during the day, provided additional and complementary information. Conclusion: The DVC® technology enabled the home cage monitoring of mice and non-invasive detection of animal activity disturbances. It can easily be integrated into a multimodal monitoring approach to better capture the different effects of oncology drugs on anti-tumor efficacy, toxicity, and safety and improve translation to clinical studies.
Pro-Apoptotic Activity of MCL-1 Inhibitor in Trametinib-Resistant Melanoma Cells Depends on Their Phenotypes and Is Modulated by Reversible Alterations Induced by Trametinib Withdrawal
Authors: Hartman Mariusz L. , Koziej Paulina , Kluszczyńska Katarzyna , Czyz Małgorzata
Published: September 2023

Background: Although BRAFV600/MEK inhibitors improved the treatment of melanoma patients, resistance is acquired almost inevitably. Methods: Trametinib withdrawal/rechallenge and MCL-1 inhibition in trametinib-resistance models displaying distinct p-ERK1/2 levels were investigated. Results: Trametinib withdrawal/rechallenge caused reversible changes in ERK1/2 activity impacting the balance between pro-survival and pro-apoptotic proteins. Reversible alterations were found in MCL-1 levels and MCL-1 inhibitors, BIM and NOXA. Taking advantage of melanoma cell dependency on MCL-1 for survival, we used S63845. While it was designed to inhibit MCL-1 activity, we showed that it also significantly reduced NOXA levels. S63845-induced apoptosis was detected as the enhancement of Annexin V-positivity, caspase-3/7 activation and histone H2AX phosphorylation. Percentages of Annexin V-positive cells were increased most efficiently in trametinib-resistant melanoma cells displaying the p-ERK1/2low/MCL-1low/BIMhigh/NOXAlow phenotype with EC50 values at concentrations as low as 0.1 μM. Higher ERK1/2 activity associated with increased MCL-1 level and reduced BIM level limited pro-apoptotic activity of S63845 further influenced by a NOXA level. Conclusions: Our study supports the notion that the efficiency of an agent designed to target a single protein can largely depend on the phenotype of cancer cells. Thus, it is important to define appropriate phenotype determinants to stratify the patients for the novel therapy.
Vegetation Dynamics of Sub-Mediterranean Low-Mountain Landscapes under Climate Change (on the Example of Southeastern Crimea)
Authors: Tabunshchik Vladimir , Gorbunov Roman , Gorbunova Tatiana , Safonova Mariia
Published: September 2023

In the context of a changing environment, understanding the interaction between vegetation and climate is crucial for assessing, predicting, and adapting to future changes in different vegetation types. Vegetation exhibits high sensitivity to external environmental factors, making this understanding particularly significant. This study utilizes geospatial analysis techniques, such as geographic information systems, to investigate vegetation dynamics based on remote sensing data and climatic variables, including annual air temperature, annual precipitation, and annual solar radiation. The research methodology encompasses data collection, processing, and analysis, incorporating multispectral imagery and multilayered maps of various parameters. The calculation of the normalized difference vegetation index serves to evaluate changes in vegetation cover, identify areas experiencing variations in green biomass, and establish strategies for the future development of different vegetation types. During the period from 2001 to 2022, the average normalized difference vegetation index value in the Southeastern Crimea region amounted to 0.443. The highest average values were recorded in the year 2006, reaching a magnitude of 0.469. Conversely, the lowest values were observed in the years 2001–2002, constituting 0.397. It has been ascertained that an overarching positive trend in the evolution of NDVI values from 2001 to 2022 is apparent, thus implying a notable augmentation in vegetative biomass. However, adversarial trends manifest in discrete locales adjacent to the cities of Sudak and Feodosia, along with the coastal stretches of the Black Sea. Correlation analysis is employed to establish relationships between vegetation changes and climatic indicators. The findings contribute to our understanding of the vulnerability of various vegetation types and ecosystems in the Southeastern Crimea region. The obtained data provide valuable insights for the development of sustainable vegetation resource management strategies and climate change adaptation in the region.