bluuebunny/crossref_metadata_2025 huggingface dataset:
β-Cyclodextrin-Modified Cotton Fabric for Medical and Hospital Applications with Photodynamic Antibacterial Activity Using Methylene Blue
Authors: Firmino Helen Beraldo , Volante Emilly Karoline Tonini Silva , da Silva Ana Claudia Pedrozo , Scacchetti Fabio Alexandre Pereira , Lis Manuel José , Martí Meritxell , Saxena Siddanth , Tessaro André Luiz , Bezerra Fabrício Maestá
Published: September 2024

The use of cyclodextrins in textiles for the development of biofunctional fabrics represents an interesting alternative for the advancement of dental, medical, and hospital materials. Cyclodextrins can interact with the chemical groups present in cotton fibers, leading to the formation of a nanostructured surface with specific functional properties, including antibacterial activity. Although there are numerous antibacterial textile finishes, the use of methylene blue as a cyclodextrin host molecule for photodynamic applications in textile materials remains to be investigated. This is because methylene blue is a photosensitive dye capable of generating singlet oxygen (1O2) when illuminated, which inactivates the pathogenic microorganisms that may be present in wounds. The objective of this study was to develop a biofunctionalized and photoactivatable cotton fabric with antimicrobial properties for use in the cosmetic or medical industries. The materials obtained were characterized via scanning electron microscopy (SEM), Fourier transform infrared spectroscopy with attenuated total reflection (FTIR-ATR), the determination of cotton fabric functionalization dyeing variables, colorimetry, UV-VIS spectrophotometry, degradation of 9,10-anthracenediyl-bis(methylene)dimalonic acid (ABDA), photodegradation tests, and microbiological analysis. The results showed that the textile was functionalized with β-cyclodextrin, mainly evidenced by the appearance of the band at 1730 cm−1, indicating the formation of the ester group. Thus, when exposed to light, the non-functionalized material showed greater photobleaching, about 60%, compared to the material treated with cyclodextrin. This result was also reflected in the ABDA degradation kinetics, with the treated material showing 592.00% (first phase) and 966.20% (second phase) higher degradation than the untreated sample. Finally, the photodynamic activity was determined based on the antimicrobial properties of the textile, showing a reduction of more than 99% without exposure to light and 100% when exposed to light. It is believed that this study could open avenues for future research and the development of antimicrobial fabrics, as well as demonstrate the efficiency of the treatment with cyclodextrin in relation to photobleaching.
Study on the Refining Behavior of Al-Si Alloys Doped with Al-Ti-C Intermediate Alloys at Different B Doping Temperatures
Authors: Liu Xiaodong , Liang Mengtian , Yin Bingbing , Yang Chuanlong , Li Yuhao , Yang Yi
Published: September 2024

In this paper, a novel Al-5Ti-0.3C-0.2B intermediate alloy with an efficient refining effect on Al-Si alloys by a low-cost and high-performance fabrication process is developed. The results show that the addition of only 0.15 wt.% of Al-5Ti-0.3C-0.2B intermediate alloy is able to refine the average grain size of Al-Si alloys to 120 ± 5 μm, which is 28.7% higher than the reference value of 0.2 wt.% addition. Additionally, TiAl3 in bulk form has a faster response and more stable refinement than TiAl3 in needle form. The blocky TiAl3 generated at low temperature will turn acicular or elongated as the temperature increases, slowing the response speed and increasing the time required to achieve the ideal refinement effect. We are convinced that this work will provide a reference for developing new aluminum alloy refinement intermediate alloys.
Oxidation Behavior of Lightweight Al0.2CrNbTiV High Entropy Alloy Coating Deposited by High-Speed Laser Cladding
Authors: Chen Tianhui , Bi Zhijiang , Zhou Ji , Shuai Ruohui , Cai Zhihai , Lou Liyan , Wang Haidou , Xing Zhiguo
Published: September 2024

High-temperature oxidation resistance is the major influence on the high-temperature service stability of refractory high entropy alloys. The oxidation behavior of lightweight Al0.2CrNbTiV refractory high entropy alloy coatings with different dilution ratios at 650 °C and 800 °C deposited by high-speed laser cladding was analyzed in this paper. The oxidation kinetic was analyzed, the oxidation resistance mechanism of the Al0.2CrNbTiV coating was clarified with the analysis of the formation and evolution of the oxidation layer, and the effect of the dilution rate on high-temperature performances was revealed. The results showed that the oxide layer was mainly composed of rutile oxides (Ti, Cr, Nb)O2 after isothermal oxidation at 650 °C and 800 °C for 50 h. The Al0.2CrNbTiV coating in low dilution exhibited better oxidation performance at 650 °C, due to the dense oxide layer formed with the synergistic growth of fine AlVO3 particles and (Ti, Cr, Nb)O2, and higher percentage of Cr, Nb in (Ti, Cr, Nb)O2 strengthened the lattice distortion effect to inhibit the penetration of oxygen. The oxide layer formed at 800 °C for the Al0.2CrNbTiV coating was relatively loose, but the oxidation performance of the coating in high dilution improved due to the precipitation of Cr2Nb-type Laves phases along grain boundaries, which inhibits the diffusion of oxygen.
Tribological Comparison of Coatings Produced by PVD Sputtering for Application on Combustion Piston Rings
Authors: Ferreira Ney Francisco , Fernandes Filipe , Neis Patric Daniel , Poletto Jean Carlos , Yaqub Talha Bin , Cavaleiro Albano , Vilhena Luis , Ramalho Amilcar
Published: September 2024

This article compares the tribological performance of coatings produced by PVD sputtering. Transition metal dichalcogenide (TMD) coatings doped with carbon (WSC and MoSeC) and nitrogen (WSN and MoSeN) and a conventional diamond-like carbon (DLC) coating are compared. The tribological evaluation was oriented towards the use of coatings on piston rings. Block-on-ring tests in a condition lubricated with an additive-free polyalphaolefin (PAO 8) and at temperatures of 30, 60, and 100 °C were carried out to evaluate the coatings in boundary lubrication conditions. A load scanner test was used to evaluate dry friction and scuffing propensity. In addition to WSN, all other TMD coatings (WSC, MoSeC, and MoSeN) exhibited lower friction than DLC in dry and lubricated conditions. The study reveals that WSC, among TMD coatings, offers promising results, with significantly lower friction levels than DLC, while demonstrating reduced wear and a lower risk of metal adhesion. These findings suggest that WSC may be a viable alternative to DLC in piston rings, with potential benefits for reducing fuel consumption and increasing engine durability.
Future Parabolic Trough Collector Absorber Coating Development and Service Lifetime Estimation
Authors: Drinčić Ana , Noč Luka , Merzel Franci , Jerman Ivan
Published: September 2024

This work presents a study on the optical and mechanical degradation of parabolic trough collector absorber coatings produced through the spray coating application technique of in-house developed paint. The main aim of this investigation is to prepare, cure, load, and analyze the absorber coating on the substrate under conditions that mimic the on-field thermal properties. This research incorporates predicted isothermal and cyclic loads for parabolic trough systems as stresses. Biweekly inspections of loaded, identical samples monitored the degradation process. We further used the cascade of data from optical, oxide-thickening, crack length, and pull-off force measurements in mathematical modelling to predict the service life of the parabolic trough collector. The results collected and used in modelling suggested that cyclic load in combination with iso-thermal load is responsible for coating fatigue, influencing the solar absorber optical values and resulting in lower energy transformation efficiency. Finally, easy-to-apply coatings made out of spinel-structured black pigment and durable binder could serve as a low-cost absorber coating replacement for a new generation of parabolic trough collectors, making it possible to harvest solar energy to provide medium-temperature heat to decarbonize future food, tobacco, and paint production industrial processes.
Controllable Crystallization of Perovskite Films during the Blade-Coating Fabrication Process for Efficient and Stable Solar Cells
Authors: Jiang Zhaoyi , Li Jiaqi , Li Kun , Zhang Rui , Qin Zhenxing , Zhang Yufei , Wu Boheng , Ma Qiang , Zhang Yulong , Zhang Weijia
Published: September 2024

The scalable production of high-quality perovskite thin films is pivotal for the industrialization of perovskite thin film solar cells. Consequently, the solvent system employed for the fabrication of large-area perovskite films via coating processes has attracted significant attention. In this study, a solvent system utilizing a volatile solvent as the primary reagent has been developed to facilitate the rapid nucleation of volatile compounds. While adding the liquid Lewis base dimethylformamide (DMF) can help to improve the microstructure of perovskite films, its slow volatilization renders the crystal growth process uncontrollable. Based on the solvent system containing DMF and ethanol (EtOH), introducing a small amount of NH4Cl increases the proportion of the intermediate phase in the precursor films. This not only results in a controllable growth process for the perovskite crystals but also contributes to the improvement of the film microstructure. Under the simulated illumination (AM1.5, 1000 W/m2), the photoelectric conversion efficiency (PCE) of the inverted solar cells has been improved to 20.12%. Furthermore, after 500 hours of continuous illumination, the photovoltaic device can retain 95.6 % of the initial, indicating that the solvent system is suitable for the scalable fabrication of high-quality FAPbI3 thin films.
The Influence of Surface Nanocrystallization of TA2 Titanium Alloy on Its Corrosion Resistance
Authors: Li Zhengyi , Wang Yanzhao , Zhang Jiangong , Guo Yiwei , Wen Lei
Published: September 2024

In this study, the surface nano treatment of TA2 titanium alloy was realized by means of surface mechanical wear treatment. The microstructure and electrochemical properties of the nanocrystalline layer were investigated by SEM, TEM, Electrochemical Impedance Spectroscopy, and Potentiodynamic Polarization, while the nucleation mechanism of the passivation film was discussed. The results indicate that the original coarse grains on the sample’s surface are transformed into randomly oriented nanocrystals by surface mechanical attrition treatment (SMAT). The corrosion current density of the surface nanocrystallized TA2 titanium alloy (9.2 nA·cm−2) experienced a reduction of two orders of magnitude compared to untreated TA2 titanium alloy (134.5 nA·cm−2) in 3.5 wt.% NaCl solution. The SMAT methods accelerates nucleation mechanism transitioning to continuous nucleation.
A New Paradigm for Semiconductor Manufacturing: Integrated Synthesis, Delivery, and Consumption of Source Chemicals for IC Fabrication
Authors: Arkles Barry , Kaloyeros Alain E.
Published: September 2024

The semiconductor industry is being radically impacted by the placing of greater emphasis on the development of hetero-devices and systems that will act as essential drivers for a wide spectrum of technological applications. The introduction of new materials and their integration with currently used materials are projected to replace integrated circuitry (IC) design and device scaling as the key enablers to the realization of improved device performance and larger density gains. Yet material selection has been constrained by existing fabrication process technology. To date, fabrication processes have dictated material selection by limiting chemical sources or precursors to those that match existing process tools associated with chemically based vapor phase processes and their variants, which in turn limits material compositions in ICs. The processing and integration of new materials compositions and structures will require the introduction of new deposition and etching processes, and manufacturing worthy tool designs and associated protocols that provide new methods for atomic-level control. To this end, a novel manufacturing paradigm is presented comprising a method and system for real-time, closed-loop monitoring and control of synthesis, supply, and consumption of precursors in process intensification techniques including chemical vapor deposition (CVD), atomic layer deposition (ALD), atomic layer etching (ALE), and other IC manufacturing processes. This intelligent automated manufacturing approach is consistent with a central component of the semiconductor industry’s recent adoption of Industry 4.0., including vertical integration of IC manufacturing through robotization, artificial intelligence, and cloud computing. Furthermore, the approach eliminates several redundant steps in the synthesis, handling, and disposal of source precursors and their byproducts for CVD, ALD, ALE and other chemically based manufacturing processes, and thus ultimately lowers the manufacturing cost for both conventional and new IC materials. Further, by eliminating the issues associated with precursor thermal, chemical, and pyrophoric instabilities, this new paradigm enables the deposition of a myriad of new thin-film materials and compositions for IC applications that are practically unattainable with existing precursors. Preliminary and planned demonstration examples for the generation and deposition of highly toxic and unstable source precursors are provided.
Preparation and Properties of Waterborne Acrylic-Modified Epoxy Phosphate Resin and Its Coating
Authors: Xiao Fei , Yong Tao , Cao Tianlong , Shi Fangyuan , Sun Xuejun , Zhang Jin
Published: September 2024

An acrylic acid-modified epoxy phosphate resin coating was synthesized by a four-step method marked “A-B-C-D”, and it was used as an efficient protective layer for steel structures. The coating exhibited good properties, mainly including water resistance (≥240 h), salt spray resistance (≥300 h), surface drying time (≤1 h), and adhesion (≥6.5 MPa).
Genetic Diversity and Distribution of Haplotypes of Freshwater Eel in Baltic Lakeland Based on Mitochondrial DNA D-Loop and Cytochrome b Sequence Variation
Authors: Oreha Jeļena , Morozova Aleksandra , Garkajs Alberts , Kirjušina Muza , Gavarāne Inese , Kostousov Vladimir G. , Pupins Mihails , Škute Nataļja
Published: September 2024

The European eel (Anguilla anguilla (L.)) is a unique catadromous euryhaline fish and the only anguillid eel species classified as ‘critically endangered’. The Baltic Lakeland area contains naturally recruited and introduced eels. There is great uncertainty regarding a baseline for the location and number of naturally dispersed eels in the region. Little is known about the genetic structure of the European eel populations in Baltic countries. The estimation of population genetic structure is important for the efficient management of naturally recruited and introduced eels. Two mitochondrial regions were used to investigate the genetic structure within and between eel samples from 11 waterbodies. In this study, new, unique, and widely distributed haplotypes were revealed. The studied eel population in the Baltic Lakeland shows high genetic diversity, which is possibly a result of intensive restocking programs. Sequences characterized for Anguilla rostrata were revealed in both mitochondrial regions. Understanding the genetic structure of eel populations worldwide is crucial for conservation efforts. Eel restocking in waterbodies where natural migration is restricted contributes to diversity loss for the world gene pool of eels.
Taxonomic, Functional, and Phylogenetic Diversity of Bats in Urban and Suburban Environments in Southern México
Authors: Briones-Salas Miguel , Medina-Cruz Gabriela E. , Martin-Regalado Cintia Natalia
Published: September 2024

Urbanization is one of the leading causes of habitat loss, which has increased significantly in tropical regions in recent years, leading to the loss of species, their ecological functions, and evolutionary history. To determine the effect of urbanization on the diversity of bat communities in urban and suburban environments, we analyzed the α and β taxonomic, functional, and phylogenetic diversities at four sites along urbanization gradients surrounding a rapidly expanding city (Oaxaca City) in southern Mexico. We recorded bats using conventional techniques such as mist nets and acoustic monitoring. We calculated the diversity of bats in four sites with different urbanization conditions: urban (1), suburban (1), and rural (2). To assess the degree of total differentiation and components of bat turnover and nestedness between sites, we calculated the β taxonomic, functional, and phylogenetic diversities. A total of 33 bat species were recorded. The highest taxonomic and functional diversity was observed in the Center of Oaxaca (the site with the highest level of urbanization). In contrast, the highest phylogenetic diversity was found in the West (the site with the lowest level of urbanization). The total β taxonomic diversity was higher than the functional and phylogenetic diversity. Regarding the contributions of turnover and nestedness, turnover made a more significant contribution than nestedness to the taxonomic and phylogenetic β diversity. In contrast, functional nestedness contributed more to the functional β diversity than turnover. Tadarida brasiliensis, Desmodus rotundus, Sturnira hondurensis, and S. parvidens were recorded in all three urbanization conditions. In the most urbanized site, four Myotis species were recorded: M. fortidens, M. keaysi, M. thysanodes, and M. velifer. We suggest that the analysis of different dimensions of diversity is essential and should be considered to strengthen conservation strategies; moreover, we suggest the preservation of native vegetation mosaics and water bodies within the city to maintain bat diversity.
Annotation and Characterization of the Zacco platypus Genome
Authors: Nam Sang-Eun , Bae Dae-Yeul , Rhee Jae-Sung
Published: September 2024

The pale chub Zacco platypus (Cypriniformes; Xenocyprididae; Jordan & Evermann, 1902) is widely distributed across freshwater ecosystems in East Asia and has been recognized as a potential model fish species for ecotoxicology and environmental monitoring. Here, a high-quality de novo genome assembly of Z. platypus was constructed through the integration of a combination of long-read Pacific Bioscience (PacBio) sequencing, short-read Illumina sequencing, and Hi-C sequencing technologies. Z. platypus has the smallest genome size compared to other species belonging to the order Cypriniformes. The assembled genome encompasses 41.45% repeat sequences. As shown in other fish, a positive correlation was observed between genome size and the composition of transposable elements (TE) in the genome. Among TEs, a relatively higher rate of DNA transposon was observed, which is a common pattern in the members of the order Cypriniformes. Functional annotation was processed using four representative databases, identifying a core set of 12,907 genes shared among them. Orthologous gene family analysis revealed that Z. platypus has experienced more gene family contraction rather than expansion compared to other Cypriniformes species. Among the uniquely expanded gene families in Z. platypus, detoxification and stress-related gene families were identified, suggesting that this species could represent a promising model for ecotoxicology and environmental monitoring. Taken together, the Z. platypus genome assembly will provide valuable data for omics-based health assessments in aquatic ecosystems, offering further insights into the environmental and ecological facets within this species.
Analysis of the Raccoon (Procyon lotor) and Common Raccoon Dog (Nyctereutes procyonoides) Spatiotemporal Changes Based on Hunting Bag Data in Hungary
Authors: Schally Gergely , Bijl Hanna , Kashyap Bhraaz , Márton Mihály , Bőti Szilvia , Katona Krisztián , Biró Zsolt , Heltai Miklós , Csányi Sándor
Published: September 2024

Sustainable wildlife management requires reliable data on population, habitat, and the interaction between them. Since 1993, Hungary has maintained the National Game Management Database, which collects data on game populations and hunting bags annually. During the last three decades, populations of medium-sized predators have remarkably increased in the country. Our study analyzed changes in hunting bag data and spatial distribution of two invasive alien species, the raccoon (Procyon lotor) and the raccoon dog (Nyctereutes procyonoides), utilizing annual game management reports from Game Management Units from 1997 to 2021. For spatial analysis, we employed a UTM grid system (10 × 10 km) covering the country. Our findings confirmed increasing trends in the hunting bag and area (number of grid cells) containing hunting bags for both species. From the first (1997–2001) to the last (2017–2021) period, the average annual hunting bag increased (raccoon: 0.40 → 11.00 ind/year; raccoon dog: 3.40 → 8.00 ind/year), and the average number of cells with hunting bags also expanded significantly (raccoon: 2.00 → 9.60 UTM cells/year; raccoon dog: 5.80 → 15.20 UTM cells/year). These trends and the size of potentially suitable habitats suggest a further spread and population increase, posing challenges for nature conservationists and wildlife managers.
Two-Decade Changes in the Ciliate Assemblage Feeding Pattern Reflect the Reservoir Nutrient Load
Authors: Macek Miroslav , Vrba Jaroslav , Hejzlar Josef , Řeháková Klára , Jarošík Jiří , Šorf Michal , Šimek Karel
Published: September 2024

The perception of the importance of ciliate in freshwater has changed dramatically since the “microbial loop” conceptualisation, reflecting methodological attempts. The data from two decades (1994–2018) on the surface (0–3 m) ciliate assemblage in the Slapy reservoir (Vltava River, Czech Republic) during two different nutrient-load defined periods were analysed. We grouped the identified, quantified, and biomass-evaluated ciliates in the quantitative protargol-impregnated preparations according to their feeding behaviour. The sampling median and interquartile range data of the ciliates were plotted; the modelled water age, nutrients, bacteria, heterotrophic nanoflagellates, and Rhodomonas spp. were applied as the main explanatory background variables. We validated the differences between the periods, engaging multivariate analyses. The picoplankton-filtering species dominated the assemblages in an annual mean (halteriids and minute strobilidiids followed by peritrichs). Algae hunting urotrichs, Balanion planctonicum, and nanoplankton filtering tintinnids were significant before the spring phytoplankton peak when a maximum of ciliate biomass reflected mixotrophic nanoplankton filtering pelagostrombidiids. Only there did ciliate biomass tightly follow their quantified prey. Heterotrophic and mixotrophic Askenasia and Lagynophrya were typical raptorial/flagellate-hunting cilates; only Mesodinium spp. reached the maximum during autumn. The observed oligotrophication of the reservoir increased the ciliate assemblage biomass in the surface layer during stratification in concordance with the Plankton Ecology Group (PEG) model.
Exposure of American Black Bears (Ursus americanus) to Ticks, Tick-Borne Diseases, and Intestinal Parasites in Wisconsin
Authors: Reichert Nika S. , Mathieu Daniela , Katz Christopher J. , Hatch Kent A.
Published: September 2024

We surveyed 159 American black bears (Ursus americanus) over a period of three years for the occurrence of ticks, tick-borne diseases, and intestinal parasites in Wisconsin. We collected blood from the bears to test for the presence of antibodies to Borrelia burgdorferi (Lyme disease), Rickettsia rickettsii (Rocky Mountain spotted fever (RMSF)), Babesia, Ehrlichia, Ehrlichia canis, Brucella canis, and Anaplasma phagocytophilum. We also examined scat samples for intestinal parasites. We commonly found the tick Dermacentor variabilis, but also present the first report of Rhipicephalus sanguineus on black bears. We detected antibodies to Lyme disease and RMSF. We detected antibodies to E. canis for the first time in a bear and both antibodies to R. rickettsii and A. phagocytophilum for the first time in a black bear in Wisconsin. No antibodies for Babesia or Br. canis were detected. We found eggs of the intestinal parasite Baylasascaris transfuga as well as a low number of Toxascara leonina and unknown Capillaria species occurrences in the examined feces.
The Diet of Eleonora’s Falcons (Falco eleonorae) during the Autumn Migration of Passerine Birds across the Aegean Sea
Authors: Ristow Dietrich , Wink Michael
Published: September 2024

Every year, several hundred million birds cross the Mediterranean on their migration from Eurasia to their wintering quarters in Africa. As many migrants travel at night or at high altitudes, direct observations of bird migration are difficult and thus our information about migrating species, numbers and timing is incomplete. An indirect way to assess autumn migration is the analysis of prey remains of Eleonora’s Falcons (Falco eleonorae). These falcons breed in large colonies on islands in the Mediterranean and on the Canary Islands. Many migrants have to pass these islands on their flight to their African wintering quarters. Eleonora’s Falcons appear to be adapted to the autumn bird migration and raise their young between August and October, when migrating birds are abundant. When nestlings have to be fed, falcons exclusively hunt small birds of 10 to 150 g body mass, whereas they prey mostly on aerial invertebrates (Coleoptera, Hymenoptera, Diptera, Orthoptera, Hemiptera, Odonata, Lepidoptera) from November to July. We studied Eleonora’s Falcons from 1965 to 2001 on a rocky islet, north of Crete, which harboured a colony of about 200 breeding pairs. In 1969, 1971, 1977, and 1988 we systematically monitored and collected the pluckings and cached food items in 22 to 36 nest sites each year. Pluckings were systematically analysed later in Germany using a reference collection of bird feathers for identification. In total, we determined more than 111 prey species (mostly Passerines) comprising more than 13,450 individuals. The top 12 prey species were: Willow Warbler (27.8% of all prey items), Red-backed Shrike (10.7%), Spotted Flycatcher (9.9%), Whinchat (8.8%), Common Whitethroat (5.1%), Wood Warbler (3.8), Tree Pipit (2.9%), Icterine Warbler (2.5%), Greater Short-toed Lark (2.5%), Northern Wheatear (1.8%), Common Nightingale (1.6%), and European Pied Flycatcher (1.5%). Eleonora’s Falcons are selective hunters to some degree; thus, the phenology and abundance data derived from the plucking analyses are biased towards slow-flying species or smaller birds (only up to a body mass of 150 g). When the young falcons develop and grow, food demand increases concomitantly. Comparing the total weight of prey over time indicates a correlation with food demand and in consequence with the number of prey items brought to the nest sites by the falcons.
Fungal Bioluminescence: Past, Present, and Future
Authors: Lu Wenhua , Priyashantha Alviti Kankanamalage Hasith , Galappaththi Mahesh C. A. , Tibpromma Saowaluck , Dai Dong-Qin , Patabendige Nimesha M. , Premarathne Bhagya M. , Kulasuriya Dinesh M. , Ediriweera Aseni N. , Nimalrathna Thilina S. , Suwannarach Nakarin , Lumyong Saisamorn , Tang Alvin , Shao Shi-Cheng , Karunarathna Samantha C.
Published: September 2024

The complex and diverse phenomenon of fungal bioluminescence has captured human curiosity. Nevertheless, in the field of studies, there are not many attempts made particularly to reveal the new species of these interesting fungi. This study comprehensively reviews the diversity, distribution, evolution, bioluminescence mechanisms, ecological roles, and potential applications of these fungi. Most importantly, we also present an updated list of the reported bioluminescent fungi (122) so far identified from five distinct evolutionary lineages worldwide—Armillaria, Eoscyphella, Lucentipes, Mycenoid, and Omphalotus—mainly in tropical and subtropical areas. Bioluminescent fungi are descended from the last common ancestor of the Mycenoid and the Marasmioid clades of Agaricales, which have been maintained for at least 160 million years of evolution. We underscore the potential for future research to understand the ecological role of bioluminescent fungi, inspiring hope and optimism for the future of this field.
The Association between the Severity of Distal Sensorimotor Polyneuropathy and Increased Carotid Atherosclerosis in Individuals with Type 2 Diabetes
Authors: Hsieh Dong-Yi , Lai Yun-Ru , Huang Chih-Cheng , Ting Chi-Ping , Chiu Wen-Chan , Chen Yung-Nien , Lien Chia-Yi , Cheng Ben-Chung , Lin Ting-Yin , Chiang Hui Ching , Lu Cheng-Hsien
Published: September 2024

Background: Diabetes contributes to a spectrum of complications encompassing microvascular and macrovascular disorders. This study aimed to explore the correlation between distal sensorimotor polyneuropathy (DSPN) severity and heightened carotid atherosclerosis among individuals with type 2 diabetes mellitus (T2DM). Method: Participants underwent comprehensive assessments including nerve conduction studies (NCS), Toronto Clinical Neuropathy Score (TCNS) evaluations, assessment of cardiometabolic risk factors, and carotid sonography studies covering dynamic and morphological parameters. The resistance index (RI), pulsatility index (PI), peak systolic velocity (PSV), and end-diastolic velocity (EDV) in both the common carotid artery (CCA) and internal carotid artery (ICA), carotid intima-media thickness (IMT), and carotid plaque score (CPS) were also measured. Peripheral nerve function severity was assessed using composite amplitude scores (CAS) derived from NCS. Results: Individuals with DSPN exhibited lower EDV in the CCA and ICA (p < 0.0001 and p = 0.002), higher PI and RI in both CCA and ICA (all p < 0.0001), and higher CPS (p = 0.002). They also demonstrated a higher prevalence of retinopathy as an underlying condition, higher index HbA1c, and reduced estimated glomerular filtration rate (eGFR) (all p < 0.0001). Multiple linear regression analysis revealed significant associations where eGFR, ICA-PI, index HbA1c, waist circumference, and age were correlated with CAS. Meanwhile, diabetes duration, waist circumference, age, and index HbA1c showed significant associations with TCNS. Conclusions: Our study suggests that individuals with T2DM who exhibit more severe carotid atherosclerosis may not only be at increased risk of developing DSPN but also may experience greater severity of DSPN. PI in both the CCA and ICA, along with the CPS, serve as surrogate biomarkers for DSPN severity.
Capillary Leak Syndrome Associated with Anaplastic T Cell Lymphoma and Transcutaneous Exudation: An Unusual Presentation
Authors: Tomai Radu Andrei , Oancea Antonia , Tomuleasa Ciprian , Dima Delia
Published: September 2024

Capillary leak syndrome is a rare complication of cancer, particularly of hematologic malignancies. The syndrome was first described as an idiopathic entity; however, increasingly, more cases are being reported in association with cancers and other conditions. Diagnosis stems from the recognition of the double paradox, consisting of severe generalized oedema and hypotension, accompanied by hallmark laboratory modifications. Concurrent conditions in patients with malignancies can alter laboratory findings and make the diagnosis a challenge. This report presents the case of a patient with capillary leak syndrome and an atypical presentation, with generalized skin rash and transcutaneous exudation occurring concurrently with anaplastic large T cell lymphoma, macrophage activation syndrome, and cytopenias. Symptom-specific treatment with diuretics and albumin was ineffective in the case of our patient; however, the CLS remitted promptly with cancer-specific therapy. No treatment has proved to be generally effective against CLS up to date, as is the case for this patient. Thus, the rapid recognition of cancer-associated capillary leak syndrome and the initiation of cancer-specific treatment proves to be the better approach and is key to avoiding unnecessary delays and ineffective treatments targeted specifically at CLS.
Blood-, Tissue- and Urine-Based Prognostic Biomarkers of Upper Tract Urothelial Carcinoma
Authors: Łaszkiewicz Jan , Krajewski Wojciech , Sójka Aleksandra , Nowak Łukasz , Chorbińska Joanna , Subiela José Daniel , Tomczak Wojciech , Del Giudice Francesco , Małkiewicz Bartosz , Szydełko Tomasz
Published: September 2024

Upper tract urothelial carcinoma (UTUC) is a rare but aggressive neoplasm. Currently, there are few reliable and widely used prognostic biomarkers of this disease. The purpose of this study was to assess the prognostic value of blood-, tissue- and urine-based biomarkers in patients with UTUC. A comprehensive literature search was conducted using the PubMed, Cochrane and Embase databases. Case reports, editorials and non-peer-reviewed literature were excluded from the analysis. As a result, 94 articles were included in this review. We evaluated the impact of 22 blood-based, 13 tissue-based and 4 urine-based biomarkers and their influence on survival outcomes. The neutrophil–lymphocyte ratio, albumin, C-reactive protein, De Ritis ratio, renal function and fibrinogen, which are currently mentioned in the European Association of Urology (EAU) guidelines, are well researched and most probably allow for a reliable prognosis estimate. However, our review highlights a number of other promising biomarkers that could potentially predict oncological outcomes in patients with UTUC. Nonetheless, the clinical value of some prognostic factors remains uncertain due to the lack of comprehensive studies.
Preoperative Cone Beam Computed Topography Assessment of Maxillary Sinus Variations in Dental Implant Patients
Authors: Misăiloaie Alexandru , Tărăboanță Ionuț , Budacu Cristian Constantin , Sava Anca
Published: September 2024

This study aimed to evaluate the pathological factors and anatomical variations in the maxillary sinus in patients undergoing dental implant treatment using cone beam computed tomography (CBCT). CBCT, as a key imaging technique in dentistry, offers high-resolution images to assess bone morphology and quality, crucial for preoperative dental implant planning. Material and methods: The study included a cohort of 200 patients recommended for CBCT as part of their preoperative evaluation. The methodology involved detailed CBCT image analysis to identify and document various anatomical variations due to pneumatization, exostosis, hypoplasia, polyps, cysts, foreign bodies, and anthroliths within the maxillary sinus. Results: Pneumatization was the most common variation, present in 77.5% of subjects. Polypoid lesions were found in 17.5% of patients, with a higher prevalence in younger age groups (57.1% in ages 20–35). Cysts and polyps affected 17.5% of subjects, predominantly males (65.7%). Anthroliths were observed in a minimal percentage (2%), and foreign bodies were found in 1.5% of the patients. Positive correlations were observed between the patient’s age and both mucosal thickness and polypoid lesions and between the patient’s gender and bone thickening (p-values < 0.05). Conclusions: The study concluded that CBCT is essential in the preoperative assessment of the maxillary sinus in dental implant candidates due to its superior imaging capabilities, allowing for the identification of critical anatomical variations and pathologies. This thorough evaluation is imperative to ensure the success of implant placement and to mitigate potential complications. However, further research with larger, more diverse populations is recommended to confirm these findings.
Scheimpflug Tomographic Indices for Classifying Normal, Down Syndrome and Clinical Keratoconus in Pediatric Patients
Authors: Souza Oliveira Renato , Gil João Quadrado , Rosa Andreia , Quadrado Maria João , Campos Mauro
Published: September 2024

The study aimed to evaluate the precision of different Pentacam indices in diagnosing keratoconus (KC) in pediatric patients with and without Down Syndrome (DS) and determine suitable cutoff values. This prospective multicenter cross-sectional study evaluated 216 eyes of 131 patients aged 6–18 years (mean age 12.5 ± 3.2 years) using Pentacam. Patients were categorized into four groups: KC, forme fruste keratoconus (FK), DS, and control, excluding DS patients with topographic KC. Receiver operating characteristic curves were generated to determine the optimal cutoff points and compare the accuracy in identifying KC and FK in patients with and without DS. In DS patients, corneal morphology resembled KC features. The most effective indices for distinguishing KC in DS patients were the average pachymetric progression index (AUC = 0.961), higher-order aberration of the anterior cornea (AUC = 0.953), anterior elevation (AUC = 0.946), posterior elevation (AUC = 0.947), index of vertical asymmetry (AUC = 0.943), and Belin/Ambrosio enhanced ectasia total derivation value (AUC = 0.941). None of the indices showed good accuracy for distinguishing FK in DS patients. The thresholds of these indices differed significantly from non-DS patients. The results highlighted the need for DS-specific cutoff values to avoid false-positive or false-negative diagnoses in this population.
Preanalytical Impact of Incomplete K2EDTA Blood Tube Filling in Molecular Biology Testing
Authors: Benati Marco , Pighi Laura , Paviati Elisa , Visconti Sara , Lippi Giuseppe , Salvagno Gian Luca
Published: September 2024

Background and aims: The aim of this study was to investigate the possible preanalytical effect of incomplete filling of blood tubes on molecular biology assays. Materials and methods: The study population consisted of 13 healthy volunteers from whom 11 mL of whole blood was collected and then distributed in different volumes (1.5, 3.0, and 6.0 mL, respectively) into three 6.0 mL spray-dried and evacuated K2EDTA blood tubes. Automated RNA extraction was performed using the Maxwell® CSC RNA Blood Kit. DNA was extracted with a MagCorePlusII, with concomitant measurement of glyceralde-hyde-3-phosphate dehydrogenase (GAPDH) gene expression. The nucleic acid concentration was calculated using the NanoDrop 1000 spectrophotometer, and purity was assessed using A260/280 and A260/230 absorbance ratios. Results: The RNA concentration was higher in the tubes filled with 1.5 and 3.0 mL of blood than in the reference 6 mL filled tube. The RNA 260/280 and RNA 260/230 ratios did not differ significantly between the differently filled blood tubes. The DNA concentration remained constant in the differently filled tubes. Compared to the 6.0 mL reference filled tube, the 1.5 mL and 3.0 mL filled blood tubes displayed a lower DNA 260/280 nm ratio. The DNA 260/230 ratio did not differ significantly in any of the variously filled tubes. Compared to the 6.0 mL reference filled blood tube, the 1.5 mL and 3.0 mL filled blood tubes showed a significant increase in the GAPDHcycle threshold. Conclusions: Our results suggest that underfilling of K2EDTA blood tubes may be a modest but analytically significant source of bias in molecular biology testing.
A Comparison of Two Methods of Dental Age Estimation in a Population of Saudi Children and Adolescents
Authors: Bakhsh Heba H. , Al-shehri Nada A. , Shahwan Alanoud , Altuwairqi Rabab , Mojaleed Faten J. , Alwaalan Ghaida , Asaad Shahad
Published: September 2024

This study aimed to compare and evaluate the accuracy of the Demirjian (DE) and the London Atlas (LAE) dental age estimation methods in a Saudi population sample. This retrospective cross-sectional study used digital radiographs from electronic health records in three different dental institutes. In total, 357 male and 354 female (ages 5–15 years) digital orthopantomograms were selected for age estimation. The mean difference between the chronological age (CA) and age estimation method among males and females was 0.03 ± 0.34 and 0.00 ± 0.34, respectively, for LAE and 0.55 ± 0.84 and 0.76 ± 0.51, respectively, for DE. The mean difference between the LAE and DE methods among males and females was 0.52 ± 0.89 and −0.76 ± 0.57, respectively. No statistically significant difference between CA and LAE was found in either males (p = 0.079) or females (p = 0.872). A statistically significant difference was found between CA and DE in both genders (p < 0.001). A statistically significant difference was found between the LAE and DE groups (p < 0.001) in both genders. An overestimation of dental age was observed with DE compared with that in CA. LAE showed higher accuracy than CA, with no clinically significant difference. Although the difference between the LAE and DE methods was insignificant, the LAE method proved to be more accurate.
Use of Laxative-Augmented Contrast Medium Increases the Accuracy in the Detection of Colorectal Neoplasms
Authors: Chen Li-Yu , Chen Jong-Dar , Chen Yen-Kung
Published: September 2024

Colonic adenomas are considered a precursor of colorectal cancer. A 75-year-old woman had a history of post-operation left breast cancer. She received an excision when the left chest wall recurred. A later FDG PET/CT scan revealed a focal intense FDG accumulation in the sigmoid, a focal mild FDG uptake in the pericolic lymph node, and a focal increased FDG accumulation in the transverse colon. A delayed FDG PET/CT scan after the per-rectal administration of the laxative-augmented contrast medium revealed a filling defect with persistent FDG uptake in the sigmoid and transverse colon and mild FDG uptake in the pericolic lymph node. In addition, more lesions were observed in the rectum and descending colon. The pathology reports showed sigmoid adenocarcinoma with lymph node metastasis, and adenomas in the transverse colon, descending colon, and rectum.
The Correlation between Cardiac Magnetic Resonance Findings and Post-COVID-19: The Impact of Myocardial Injury on Quality of Life
Authors: Popovic Maja , Cvetic Vladimir , Popadic Viseslav , Ilic Kristina , Radojevic Aleksandra , Klasnja Andrea , Milic Natasa , Rajovic Nina , Lasica Ratko , Gostiljac Drasko , Klasnja Slobodan , Mahmutovic Edvin , Zdravkovic Marija
Published: September 2024

Background: In the post-COVID-19 era, there is growing concern regarding its impact on cardiovascular health and the following effects on the overall quality of life of affected individuals. This research seeks to investigate cardiac magnetic resonance (CMR) findings following COVID-19 and their impact on the quality of life of affected individuals. Methods: An observational, cross-sectional study was conducted in consecutive patients with persistent cardiovascular symptoms after COVID-19 who were referred to CMR due to suspected myocardial injury. In addition, patients completed a questionnaire about symptoms and the quality of life during the post-COVID-19 period. Results: In this study, 85 patients were included. The study population consisted of patients with a mean age of 42.5 ± 13.4 years, predominantly women, who made up 69.4% of the study population, while men made up 30.6%. CMR findings showed non-ischemic myocardial injury in 78.8% of patients and myocardial edema in 14.1% of patients. Late pericardial enhancement was present in 40% of patients and pericardial effusion in 51.8% of patients. Pericardial effusion (p = 0.001) was more prevalent in patients who reported more pronounced symptoms in the post-COVID-19 period compared to the acute infection phase. Predictors of lower quality of life in the post-COVID-19 period were the presence of irregular heartbeat (p = 0.039), cardiovascular problems that last longer than 12 weeks (p = 0.018), and the presence of pericardial effusion (p = 0.037). Conclusion: Acute myocarditis was observed in a minority of patients after COVID-19, while non-ischemic LGE pattern and pericardial effusion were observed in the majority. Quality of life was worse during the post-COVID-19 period in patients with CMR abnormalities, primarily in patients with pericardial effusion. Also, irregular heartbeat, cardiovascular symptoms that last longer than 12 weeks, as well as pericardial effusion were independent predictors of lower quality of life during the post-COVID-19 period.
Assays for Monitoring Apixaban and Rivaroxaban in Emergency Settings, State-of-the-Art Routine Analysis, and Volumetric Absorptive Microsamples Deliver Discordant Results
Authors: Fehér Adrienne , Vincze István , Rudge James , Domján Gyula , Vásárhelyi Barna , Karvaly Gellért Balázs
Published: September 2024

Our aim was to compare the performance of complementary clinical laboratory approaches to monitoring exposure to apixaban and rivaroxaban, the most prescribed direct-acting oral anticoagulants (DOAC’s): an automated commercial anti-Xa chromogenic assay suitable for emergency and pre-surgery testing and a laboratory-developed liquid chromatography-tandem mass spectrometry (LC-MS/MS) method employed for non-emergency analysis in plasma and in dried blood volumetric absorptive microsamples (VAMS) collectible by the patients in their homes. The full validation of the LC-MS/MS method was performed. Cross-validation of the methodologies was accomplished by processing 60 specimens collected for whole blood count and DOAC monitoring in a central clinical laboratory. For VAMS samples, dried plasma and whole blood calibrators were found to be suitable, and a cycle run for seven days could be implemented for rational and economic sample processing. The anti-Xa chromogrenic assay and the LC-MS/MS method delivered discordant plasma analyte concentrations. Moreover, the lack of agreement between plasma and VAMS concentrations was observed. Clinical laboratories must be aware of the differences between the performance of apixaban and rivaroxaban LC-MS/MS and anti-Xa assays. Hematocrit must always be measured along with VAMS samples to obtain accurate results.
HSP-YOLOv8: UAV Aerial Photography Small Target Detection Algorithm
Authors: Zhang Heng , Sun Wei , Sun Changhao , He Ruofei , Zhang Yumeng
Published: September 2024

To address the larger numbers of small objects and the issues of occlusion and clustering in UAV aerial photography, which can lead to false positives and missed detections, we propose an improved small object detection algorithm for UAV aerial scenarios called YOLOv8 with tiny prediction head and Space-to-Depth Convolution (HSP-YOLOv8). Firstly, a tiny prediction head specifically for small targets is added to provide higher-resolution feature mapping, enabling better predictions. Secondly, we designed the Space-to-Depth Convolution (SPD-Conv) module to mitigate the loss of small target feature information and enhance the robustness of feature information. Lastly, soft non-maximum suppression (Soft-NMS) is used in the post-processing stage to improve accuracy by significantly reducing false positives in the detection results. In experiments on the Visdrone2019 dataset, the improved algorithm increased the detection precision mAP0.5 and mAP0.5:0.95 values by 11% and 9.8%, respectively, compared to the baseline model YOLOv8s.