bluuebunny/crossref_metadata_2025 huggingface dataset:
Sentinel Lymph Node Mapping by Retroperitoneal vNOTES for Uterus-Confined Malignancies: A Standardized 10-Step Approach
Authors: Huber Daniela , Hurni Yannick
Published: June 2024

(1) Background: Sentinel lymph node (SLN) mapping represents an accurate and feasible technique for the surgical staging of endometrial and cervical cancer. This is commonly performed by conventional laparoscopy or robotic-assisted laparoscopy, but in recent years, a new retroperitoneal transvaginal natural orifice transluminal endoscopic surgery (vNOTES) approach has been described and developed by Jan Baekelandt. This technique provides easy visualization of lymphatic afferent vessels and pelvic lymph nodes, early SLN assessment, and a coherent mapping methodology following the lymphatic flow from caudal to cranial. However, only a few publications have reported it. Following the IDEAL (Idea Development Exploration Assessment Long-term follow-up) framework, research concerning this technique is in Stage 2a, with only small case series as evidence of its feasibility. Its standardized description appears necessary to provide the surgical homogeneity required to move further. (2) Methods: Description of a standardized approach for retroperitoneal pelvic SLN mapping by vNOTES. (3) Results: We describe a 10-step approach to successfully perform retroperitoneal vNOTES SLN mapping, including pre-, intra-, and postoperative management. (4) Conclusions: This IDEAL Stage 2a study could help other surgeons approach this new technique, and it proposes a common methodology necessary for evolving through future IDEAL Stage 2b (multi-center studies) and Stage 3 (randomized controlled trials) studies.
Voices from the North: Exploring Sámi People’s Perspectives on Environmental Change and Mental Well-Being: A Systematic Literature Review
Authors: Venhof Valesca S. M. , Stephens Carolyn , Martens Pim
Published: June 2024

Circumpolar Indigenous People, such as the Sámi, confront significant challenges stemming from environmental shifts and interrelated issues, profoundly affecting their mental health. Nonetheless, they possess invaluable knowledge and capabilities to navigate and adapt to these transformations. This review aims to investigate peer-reviewed scientific literature, exploring the nexus between environmental changes and mental well-being within the broader Circumpolar Indigenous community, with a special focus on the Sámi People. Conducting a systematic literature review with two arms, one encompassing the broader Circumpolar Indigenous population and the other focusing specifically on the Sámi, followed by thematic analysis, we delved into their experiences of environmental changes, perceptions regarding the intertwining of environmental shifts and mental well-being, and insights into protective factors and resilience-promoting elements. By engaging with Indigenous perspectives, public health initiatives can pinpoint and leverage existing strengths within Indigenous communities and families to bolster their ability to navigate environmental shifts and safeguard mental well-being. However, our review highlighted a lack of scientific investigation of ’strength-based’ factors fostering mental resilience among Indigenous populations inhabiting the Circumpolar North, such as the Sámi. Future qualitative research can address this gap, incorporating the viewpoints of individual Circumpolar Indigenous groups to explore both their distinctiveness and interconnectedness.
Unveiling Child Sexual Abuse Disclosure in China: An Ecological Exploration of Survivors’ Experiences
Authors: Tian Tian , Katz Ilan , Shang Xiaoyuan
Published: June 2024

Through a thematic analysis of firsthand posts from 258 abuse survivors in online forums from 2016 to 2023, this research examines the barriers that Chinese children encounter when disclosing sexual abuse. The anonymous narratives shed light on the motives behind survivors’ reluctance to reveal abuse, the outcomes following disclosure, and the wider implications for survivors and their families under culture. The findings underscore the need for early intervention upon disclosure, aiming to safeguard children from further harm and foster the development of an effective child protection framework.
Evaluation and Comparison of the Efficacy of Subcutaneous and Sublingual Immunotherapy for the Treatment of Allergic Asthma in Children
Authors: Berce Vojko , Cugmas Maša , Čopi Staša , Koren Brigita , Tomazin Maja , Hojnik Tina
Published: June 2024

Specific immunotherapy represents the only potentially curative treatment for allergic asthma. Allergens can be administered subcutaneously (SCIT) or sublingually (SLIT). The aim of the current study was to evaluate and compare the efficacy of SCIT and SLIT for the treatment of allergic asthma in children. Our study included 69 children with allergic asthma who underwent immunotherapy for house dust mites or pollen for at least 3 consecutive years. After 3 years of SCIT and SLIT, the median number of asthma exacerbations in the last three months decreased from 2 to 0 (p < 0.01) and from 1 to 0 (p < 0.01), respectively. When comparing the efficacy of SCIT and SLIT, our study revealed a significantly better efficacy of SCIT only in terms of increasing lung function. The median increase in forced expiratory volume in one second (FEV1) after 3 years was 8% with SCIT and −1% with SLIT (p < 0.01). Daily controller therapy could be withdrawn or reduced in 9 out of 16 (56.3%) children who received it before SCIT (p < 0.01) and in 19 of 29 (65.6%) children who received it before SLIT (p < 0.01), but the difference in efficacy was not significant (p = 0.88). Both SCIT and SLIT are effective treatments for allergic asthma in children.
The Comparative Efficacy and Safety of 250 μm versus 350 μm Long Microneedle Patch on Under-Eye Skin
Authors: Arepagorn Apisama , Meephansan Jitlada , Sirithanabadeekul Punyaphat , Tantisantisom Kittipong , Thongma Sattra , Rayanasukha Yossawat , Boonkoom Thitikorn , Khanchaitit Paisan
Published: June 2024

Background: Microneedle patch (MNP) technology is now applied for many purposes, including transdermal drug delivery and percutaneous collagen induction in the cosmetic and dermatology fields. Previous research showed that a MNP effectively improved skin appearance, while treatments using larger or deeper microneedles were not easily tolerated by human subjects. Few studies have compared MNP designs in humans. Study Objective: To compare novel MNP designs with high length and low density versus low length and high density for rejuvenating skin wrinkles under the eyes. Methods: This non-randomized split-face clinical trial was conducted as a double-blind study with 36 Thai female participants. Each participant was treated with two different MNP designs, one on each side of the face. The microneedle lengths were 250 μm with a density of 945 needles/cm2 on the left side of the face under the eye and 350 μm with a density of 482 needles/cm2 on the right side of the face under the eye. The treatments were applied for 12 weeks, with the assessment outcomes evaluated at the baseline and 2, 4, 6, 8, 10, and 12 weeks. Results: The application of these two novel MNP designs successfully rejuvenated under-eye wrinkles with low pain level scores. Increasing the length of the needle or having a 350 μm long MN can better reduce under-eye wrinkles without statistical significance. During the study period, there was an improvement in skin surface roughness in both groups accompanied by a consistent reduction in under-eye skin wrinkles, without statistically significant differences observed between the groups when using the Antera 3D system. However, the 350 μm long MN also slightly increased the pain compared to the shorter needles (250 μm long MN) with a higher density of needles. There were no side effects associated with the two designs. Conclusions: The two novel MNPs gave favorable results as a safe non-invasive treatment for the rejuvenation of skin wrinkles under the eyes. Increasing the number of needles and increasing the length of the needles were both effective in safely reducing under-eye wrinkles without any adverse effects. Additionally, participants could self-apply them at home and were highly satisfied. However, increasing the length of the needles may result in slightly more pain compared to increasing the number of needles.
Electrospray-Mangiferin Nanoparticles Gel: A Promising Agent for Sun and Age Defense
Authors: Chomchoei Neungreuthai , Leelapornpisid Pimporn , Tipduangta Pratchaya , Sirithunyalug Jakkapan , Sirithunyalug Busaban , Samutrtai Pawitrabhorn
Published: June 2024

UV irradiation causes skin damage and aging. This study aimed to develop and evaluate a gel formulation loaded with electrospray mangiferin nanoparticles (MNPs) as a double-action product with photoprotective and anti-aging properties. The MNPs were prepared using the electrospraying technique and loaded in a gel formulation. The MNP formulation was evaluated regarding its physical appearance, viscosity, in vitro sun protection factor (SPF), and in vitro anti-oxidant activity and compared with a formulation containing purified mangiferin (PM) at the same concentration of 0.2% (w/v). Moreover, both formulations were analyzed for their in vitro release and ex vivo skin permeation. The MNP formulation had a considerably higher SPF value than the PM formulation at the same concentration (20.43 ± 0.13 and 12.19 ± 0.27, respectively). The in vitro anti-oxidant activities of the formulations with MNPs and PM were 74.47 ± 2.19% and 80.52 ± 1.05%, respectively. The MNP formulation showed potent photoprotective and anti-oxidation activities with acceptable stability in all parameters under accelerated conditions (4 ± 2 °C 48 h/45 ± 2 °C 48 h for 6 cycles) and after 30 days of storage under various conditions. The release profile data of the MNPs showed a controlled release pattern at 76.97 ± 0.06% at 480 min. Furthermore, after using a Franz diffusion cell for 8 h, the MNP formulation showed the release of 37.01 ± 2.61% and 22.39 ± 1.59% of mangiferin content in the skin layer as stratum corneum and viable epidermis, respectively. Therefore, the overall results demonstrate that electrospray MNPs in a gel formulation are suitable for skin and constitute a promising delivery system for mangiferin in developing cosmetics and cosmeceutical products with good potential.
Abstracts of the 2024 Annual Meeting of the Swiss Neurological Society (SNS): Quo Vadis Neuroinflammation? From Pathophysiologic Advances to Novel Treatment Strategies
Authors:   Swiss Neurological Society (SNS)
Published: June 2024

On behalf of the SNS, we are pleased to present the Abstracts of the Annual Meeting which is held at the Congress Center in Basel, Switzerland, from 6–7 June 2024. In total, 83 abstracts were selected, whereof we include 8 abstracts for the Plenary Sessions, 6 abstracts for the SAYN GemSession, 30 abstracts for Poster flash presentations, and 39 abstracts as ePosters. We congratulate all the presenters on their research work and contributions.
Assessing the Extent and Severity of the Impact on Forest Soils of Two Different Fully Mechanized Timber Harvesting Operations
Authors: Lee Eunjai , Kim Taehyung , Mun Ho-Seong , Oh Jae-Heun , Han Sang-Kyun
Published: June 2024

Recently, the use of powerful and heavy vehicles for timber harvesting on flat or slightly sloping terrains has been widely expanded to provide safe working conditions and high productivity. However, soil disturbances during ground-based mechanized operations in South Korea are not fully investigated and difficult to avoid. Therefore, we compared the soil displacement and compaction (bulk density and hydraulic conductivity) between two different operations: cut-to-length (CTL) logging with a harvester and forwarder, and whole-tree (WTH) logging with a harvester and skidder. After clear-cutting, severe visual disturbances and rut depths were more prevalent in the forwarding trails than in the skidding trails. The CTL harvesting method created larger amounts of slash (6.9 kg/m2) along the trails than the WTH harvesting did (1.8 kg/m2). We found a significant difference in the compaction between the reference and the track and a negative correlation between the slash quantity values and the percentage increase in compaction. Our results showed that using skidding extraction can cause more severe impacts than forwarding extraction. Thus, these results may be helpful in understanding the influence of ground-based CTL and WTH harvesting operations and achieving best practices to minimize the environmental impacts on soil.
Seed Propagation of Carapa amorphocarpa W. Palacios Using Various Treatments of Substrates and Mechanical Seed Scarification in a Nursery in the Andean Area of Northwestern Ecuador
Authors: Villota-González Freddy Hernán , Palacios Walter , Villota González Edison Javier , Bernaola-Paucar Rosario Marilú , Sulbarán-Rangel Belkis
Published: June 2024

Carapa amorphocarpa W. Palacios is a forest species known solely from Cerro Golondrinas in northwestern Ecuador. The species is threatened due to illegal exploitation of its wood and the encroachment of the agricultural frontier. Although information on its ecology and forestry was presented in 2012, there is still very little information available. This study investigated the effects of various pre-germinative treatments on the seed germination and early growth of Carapa amorphocarpa in a nursery. The treatments included combinations of substrates (forest soil alone and forest soil mixed with sand), along with pre-germinative mechanical seed scarification. Through these treatments, the seeds’ germination capacity, growth potential, and survival rates were evaluated in a nursery. Seed germination was found to be cryptocotylar hypogeal, commencing at 41 days and achieving an average of 83.13%. Survival of germinated seedlings was 99.23% at 60 days after germination. Furthermore, a high degree of correlation in growth was observed between basal diameter and plant height (R = 0.94). The mean values for the plants’ basal diameter and total height were 0.91 cm and 50.48 cm, respectively, at 60 days. Plant form was straight with excellent health. These insights offer the potential to enhance species populations, mitigate threat risks, and in the long term, foster sustainable use of Carapa amorphocarpa genetic resources. Moreover, this seed propagation information can be leveraged for other species within the genus exploited for oil extraction from their seeds, thereby broadening knowledge and facilitating sustainable forest resource management.
Sustainable Development of Food Waste Biorefineries
Authors: García-Morales José Luis , Fernández-Morales Francisco Jesús
Published: June 2024

The sustainable development of food waste biorefineries is crucial for a number of reasons, and these reasons have environmental, economic, and social dimensions [...]
Construction of a Colorimetric and Near-Infrared Ratiometric Fluorescent Sensor and Portable Sensing System for On-Site Quantitative Measurement of Sulfite in Food
Authors: Chen Xiaodong , Zhao Chenglu , Zhao Qiwei , Yang Yunfei , Yang Sanxiu , Zhang Rumeng , Wang Yuqing , Wang Kun , Qian Jing , Long Lingliang
Published: June 2024

Sulfites play imperative roles in food crops and food products, serving as sulfur nutrients for food crops and as food additives in various foods. It is necessary to develop an effective method for the on-site quantification of sulfites in food samples. Here, 7-(diethylamino) quinoline is used as a fluorescent group and electron donor, alongside the pyridinium salt group as an electron acceptor and the C=C bond as the sulfite-specific recognition group. We present a novel fluorescent sensor based on a mechanism that modulates the efficiency of intramolecular charge transfer (ICT), CY, for on-site quantitative measurement of sulfite in food. The fluorescent sensor itself exhibited fluorescence in the near-infrared light (NIR) region, effectively minimizing the interference of background fluorescence in food samples. Upon exposure to sulfite, the sensor CY displayed a ratiometric fluorescence response (I447/I692) with a high sensitivity (LOD = 0.061 μM), enabling accurate quantitative measurements in complex food environments. Moreover, sensor CY also displayed a colorimetric response to sulfite, making sensor CY measure sulfite in both fluorescence and colorimetric dual-signal modes. Sensor CY has been utilized for quantitatively measuring sulfite in red wine and sugar with recoveries between 99.65% and 101.90%, and the RSD was below 4.0%. The sulfite concentrations in live cells and zebrafish were also monitored via fluorescence imaging. Moreover, the sulfite assimilated by lettuce leaves was monitored, and the results demonstrated that excessive sulfite in leaf tissue could lead to leaf tissue damage. In addition, the sulfate-transformed sulfite in lettuce stem tissue was tracked, providing valuable insights for evaluating sulfur nutrients in food crops. More importantly, to accomplish the on-site quantitative measurement of sulfite in food samples, a portable sensing system was prepared. Sensor CY and the portable sensing system were successfully used for the on-site quantitative measurement of sulfite in food.
A 3D-Printed Integrated Handheld Biosensor for the Detection of Vibrio parahaemolyticus
Authors: Xu Yuancong , Zhang Qian , Li Yunyi , Pang Xiaoxu , Cheng Nan
Published: June 2024

Vibrio parahaemolyticus (V. parahaemolyticus) is one of the important seafood-borne pathogens that cause a serious gastrointestinal disorder in humans. Recently, biosensors have attracted serious attention for precisely detecting and tracking risk factors in foods. However, a major consideration when fabricating biosensors is to match the low cost of portable devices to broaden its application. In this study, a 3D-printed integrated handheld biosensor (IHB) that combines RPA-CRISPR/Cas12a, a lateral flow strip (LFS), and a handheld device was developed for the ultrasensitive detection of V. parahaemolyticus. Using the preamplification of RPA on tlh gene of V. parahaemolyticus, a specific duplex DNA product was obtained to activate the trans-cleavage activity of CRISPR/Cas12a, which was then utilized to cleave the ssDNA probe. The ssDNA probe was then detected by the LFS, which was negatively correlated with the content of amplified RPA products of the tlh gene. The IHB showed high selectivity and excellent sensitivity for V. parahaemolyticus detection, and the limit of detection was 4.9 CFU/mL. The IHB also demonstrated great promise for the screening of V. parahaemolyticus in samples and had the potential to be applied to the rapid screening of other pathogen risks for seafood and marine environmental safety.
Identification of a Novel Chitinase from Bacillus paralicheniformis: Gene Mining, Sequence Analysis, and Enzymatic Characterization
Authors: Ma Xianwen , Zou Dian , Ji Anying , Jiang Cong , Zhao Ziyue , Ding Xiaoqi , Han Zongchen , Bao Pengfei , Chen Kang , Ma Aimin , Wei Xuetuan
Published: June 2024

In this study, a novel strain for degrading chitin was identified as Bacillus paralicheniformis HL37, and the key chitinase CH1 was firstly mined through recombinant expression in Bacillus amyloliquefaciens HZ12. Subsequently, the sequence composition and catalytic mechanism of CH1 protein were analyzed. The molecular docking indicated that the triplet of Asp526, Asp528, and Glu530 was a catalytic active center. The enzymatic properties analysis revealed that the optimal reaction temperature and pH was 65 °C and 6.0, respectively. Especially, the chitinase activity showed no significant change below 55 °C and it could maintain over 60% activity after exposure to 85 °C for 30 min. Moreover, the optimal host strain and signal peptide were obtained to enhance the expression of chitinase CH1 significantly. As far as we know, it was the first time finding the highly efficient chitin-degrading enzymes in B. paralicheniformis, and detailed explanations were provided on the catalytic mechanism and enzymatic properties on CH1.
Utilization of qPCR to Determine Duration and Environmental Drivers Contributing to the Persistence of Human DNA in Soil
Authors: Noel Hannah L. , George Rebecca L. , Bintz Brittania , Hickman Maureen Peters , West Frankie
Published: June 2024

Little is known about the underlying mechanisms that contribute to the persistence and degradation of DNA within soil. The goals of this study are to determine the duration of mitochondrial DNA (mtDNA) and nuclear DNA (nuDNA) persistence in soils enriched by surface-level human decomposition and to better understand the contribution of environmental factors. The surface-level decomposition of three human cadavers was documented over 11 weeks. Based on quantitative PCR results, we found nuDNA to persist in soils six weeks post-placement, while mtDNA was recoverable for the entire 11-week decomposition period. Principle components analyses and Spearman’s rank correlations revealed that (1) time, (2) total body score, and (3) weekly average air temperature were significantly correlated with concentrations of nuDNA and mtDNA in soil, suggesting these factors play a role in the degradation of DNA in soils.
Human Endogenous Retroviruses in Neurodegenerative Diseases
Authors: Adler Gabrielle L. , Le Kelvin , Fu YuHong , Kim Woojin Scott
Published: June 2024

Human endogenous retroviruses (HERVs) are DNA transposable elements that have integrated into the human genome via an ancestral germline infection. The potential importance of HERVs is underscored by the fact that they comprise approximately 8% of the human genome. HERVs have been implicated in the pathogenesis of neurodegenerative diseases, a group of CNS diseases characterized by a progressive loss of structure and function of neurons, resulting in cell death and multiple physiological dysfunctions. Much evidence indicates that HERVs are initiators or drivers of neurodegenerative processes in multiple sclerosis and amyotrophic lateral sclerosis, and clinical trials have been designed to target HERVs. In recent years, the role of HERVs has been explored in other major neurodegenerative diseases, including frontotemporal dementia, Alzheimer’s disease and Parkinson’s disease, with some interesting discoveries. This review summarizes and evaluates the past and current research on HERVs in neurodegenerative diseases. It discusses the potential role of HERVs in disease manifestation and neurodegeneration. It critically reviews antiretroviral strategies used in the therapeutic intervention of neurodegenerative diseases.
Epistemological Flexibility in Person-Centered Care: The Cynefin Framework for (Re)Integrating Indigenous Body Representations in Manual Therapy
Authors: Zegarra-Parodi Rafael , D’Alessandro Giandomenico , Baroni Francesca , Swidrovich Jaris , Mehl-Madrona Lewis , Gordon Travis , Ciullo Luigi , Castel Emiliano , Lunghi Christian
Published: June 2024

Background: Chiropractic, osteopathy, and physiotherapy (COP) professionals regulated outside the United States traditionally incorporate hands-on procedures aligned with their historical principles to guide patient care. However, some authors in COP research advocate a pan-professional, evidence-informed, patient-centered approach to musculoskeletal care, emphasizing hands-off management of patients through education and exercise therapy. The extent to which non-Western sociocultural beliefs about body representations in health and disease, including Indigenous beliefs, could influence the patient–practitioner dyad and affect the interpretation of pillars of evidence-informed practice, such as patient-centered care and patient expectations, remains unknown. Methods: our perspective paper combines the best available evidence with expert insights and unique viewpoints to address gaps in the scientific literature and inform an interdisciplinary readership. Results: A COP pan-professional approach tends to marginalize approaches, such as prevention-oriented clinical scenarios traditionally advocated by osteopathic practitioners for patients with non-Western sociocultural health assumptions. The Cynefin framework was introduced as a decision-making tool to aid clinicians in managing complex clinical scenarios and promoting evidence-informed, patient-centered, and culturally sensitive care. Conclusion: Epistemological flexibility is historically rooted in osteopathic care, due to his Indigenous roots. It is imperative to reintroduce conceptual and operative clinical frameworks that better address contemporary health needs, promote inclusion and equality in healthcare, and enhance the quality of manual therapy services beyond COP’s Western-centered perspective.
Skin Hypopigmentation in Hematology Disorders
Authors: Mazzetto Roberto , Miceli Paola , Sernicola Alvise , Tartaglia Jacopo , Alaibac Mauro
Published: June 2024

Hypopigmentation disorders pose significant diagnostic challenges in dermatology, sometimes reflecting underlying hematological conditions. This review explores the clinical presentations related to hypopigmentation in hematological disorders, focusing on vitiligo, morphea, and syndromic albinism. Vitiligo, an autoimmune disorder targeting melanocytes, involves interactions between genetic polymorphisms and immune responses, particularly regarding CD8+ T cells and IFN-γ. Drug-induced vitiligo, notably by immune checkpoint inhibitors and small-molecule targeted anticancer therapies, underscores the importance of immune dysregulation. Morphea, an inflammatory skin disorder, may signal hematological involvement, as seen in deep morphea and post-radiotherapy lesions. Syndromic albinism, linked to various genetic mutations affecting melanin production, often presents with hematologic abnormalities. Treatment approaches focus on targeting the immune pathways specific to the condition, and when that is not possible, managing symptoms. Understanding these dermatological manifestations is crucial for the timely diagnosis and management of hematological disorders.
The Molecular Biology Analysis for the Growing and Development of Hydrangea macrophylla ‘Endless Summer’ under Different Light and Temperature Conditions
Authors: Li Zheng , Lyu Tong , Lyu Yingmin
Published: June 2024

Hydrangea macrophylla, a celebrated ornamental worldwide, thrives in semi-shaded growth environments in its natural habitat. This study utilizes Hydrangea macrophylla ‘Endless Summer’ as the experimental material to delve into its molecular mechanisms for adapting to semi-shaded conditions. Transcriptome analysis was conducted on leaves from four different natural light growth scenarios, showcasing phenotypic variations. From each sample, we obtained over 276,305,940 clean reads. Following de novo assembly and quantitative assessment, 88,575 unigenes were generated, with an average length of 976 bp. Gene ontology analysis of each control group elucidated the terms associated with the suitable environmental conditions for normal growth, development, and flowering, such as “reproductive bud system development” and “signal transduction”. The exploration of gene interactions and the identification of key genes with strong connectivity were achieved by constructing a protein–protein interaction (PPI) network. The results indicate that hydrangea grows vigorously and blooms steadily under semi-shaded conditions; the photosynthetic efficiency of hydrangea is stabilized through genes related to photosynthesis, such as PHYB, PSBR, FDC, etc. Hormone signal transduction genes like PIN3, LAX2, TIF6B, and EIN3 play important roles in responding to environmental stimulation and regulating growth and development, while genes such as SOC1, COL4/5/16, and AGL24 promote flowering. The expression of genes such as BGLUs and TPSs provides additional energy substances to support flowering.
Heat Risk Perceptions and Coping Strategies of the Unhoused
Authors: Robertson Brett W. , Dow Kirstin , Salinas Julie , Cutter Susan L.
Published: June 2024

The escalating awareness of heat-related risks and the associated imperative to enhance preparedness strategies at various levels has spurred a growing emphasis on disseminating knowledge about heat vulnerability. These efforts aim to equip diverse stakeholders with practical heat planning and forecasting tools. The success of these communication initiatives hinges on understanding the nuanced perceptions of risk and the priority assigned to addressing heat as a health risk. This paper delves explicitly into the unhoused population’s risk perceptions and coping strategies. Acknowledged as one of the most underserved and vulnerable groups to extreme heat, unhoused individuals face prolonged exposure, a heightened frequency of mental and physical health issues, and limited coping resources during extreme heat events. Despite widespread acknowledgment of their vulnerability, little attention has been directed towards researching health behavior within this demographic about excessive heat. We developed a survey instrument using the theory of planned behavior (TPB) to bridge this gap. We collected quantitative survey data from unhoused populations in Columbia, South Carolina, an area of the United States that experiences extreme heat events and has a sizeable unhoused population. Using a series of hierarchical multiple regression models, our findings indicate that TPB variables predict the intention to avoid the heat while considering additional coping strategies common among unhoused individuals. These findings offer valuable insights for public health researchers, practitioners, and community officials engaged in direct interactions with unhoused populations, informing how this underserved group manages excessive heat exposure.
Medicinal Anti-Inflammatory Patch Loaded with Lavender Essential Oil
Authors: Zyburtowicz Karolina , Bednarczyk Paulina , Nowak Anna , Muzykiewicz-Szymańska Anna , Kucharski Łukasz , Wesołowska Aneta , Ossowicz-Rupniewska Paula
Published: June 2024

Transdermal drug delivery offers a promising alternative for administering medications like ibuprofen, known for its analgesic and anti-inflammatory properties, with reduced gastrointestinal side effects compared to oral administration. This study explored the potential synergistic effects of combining ibuprofen with lavender essential oil (LEO) in transdermal patches. The composition of LEO was analyzed, revealing predominant compounds such as linalyl acetate and linalool, which are known for their analgesic and anti-inflammatory properties. The physicochemical properties of the patches were investigated, indicating improved cohesion with the addition of LEO. Additionally, thermal stability assessments demonstrated enhanced stability with LEO incorporation with an increase in onset decomposition temperature from 49.0 to 67.9 °C. The antioxidant activity of patches containing LEO was significantly higher with a free radical scavenging ability of 79.13% RSA compared to 60% RSA in patches without LEO. Release and permeation studies showed that patches with LEO exhibited an increased permeation of ibuprofen through the skin with 74.40% of the drug released from LEO-containing patches compared to 36.29% from patches without LEO after 24 h. Moreover, the permeation rate was notably faster with LEO, indicating quicker therapeutic effects. The inclusion of LEO in transdermal patches containing ibuprofen holds promise for enhancing drug delivery efficiency and therapeutic effectiveness, offering a potential strategy for improved pain management with reduced side effects.
Molecular Research of Regulation of Red Blood Cells in Health, Hereditary or Acquired Diseases
Authors: Wautier Jean-Luc
Published: June 2024

During the first era of humanity, the conditions of life, including hunting, fighting, obtaining food, and diseases, were associated with frequent hemorrhages, anemia, and infections, which led to death or untreatable conditions [...]
Emerging Trends in Non-Protein Amino Acids as Potential Priming Agents: Implications for Stress Management Strategies and Unveiling Their Regulatory Functions
Authors: Decsi Kincső , Ahmed Mostafa , Rizk Roquia , Abdul-Hamid Donia , Kovács Gergő Péter , Tóth Zoltán
Published: June 2024

Plants endure the repercussions of environmental stress. As the advancement of global climate change continues, it is increasingly crucial to protect against abiotic and biotic stress effects. Some naturally occurring plant compounds can be used effectively to protect the plants. By externally applying priming compounds, plants can be prompted to trigger their defensive mechanisms, resulting in improved immune system effectiveness. This review article examines the possibilities of utilizing exogenous alpha-, beta-, and gamma-aminobutyric acid (AABA, BABA, and GABA), which are non-protein amino acids (NPAAs) that are produced naturally in plants during instances of stress. The article additionally presents a concise overview of the studies’ discoveries on this topic, assesses the particular fields in which they might be implemented, and proposes new avenues for future investigation.
Unraveling Differences in Molecular Mechanisms and Immunological Contrasts between Squamous Cell Carcinoma and Adenocarcinoma of the Cervix
Authors: Salarzaei Morteza , van de Laar Ralf L. O. , Ewing-Graham Patricia C. , Najjary Shiva , van Esch Edith , van Beekhuizen Heleen J. , Mustafa Dana A. M.
Published: June 2024

This study aims to refine our understanding of the inherent heterogeneity in cervical cancer by exploring differential gene expression profiles, immune cell infiltration dynamics, and implicated signaling pathways in the two predominant histological types of cervix carcinoma, Squamous Cell Carcinoma (SCC) and Adenocarcinoma (ADC). Targeted gene expression data that were previously generated from samples of primary cervical cancer were re-analyzed. The samples were grouped based on their histopathology, comparing SCC to ADC. Each tumor in the study was confirmed to be high risk human papilloma virus (hrHPV) positive. A total of 21 cervical cancer samples were included, with 11 cases of SCC and 10 of ADC. Data analysis revealed a total of 26 differentially expressed genes, with 19 genes being overexpressed in SCC compared to ADC (Benjamini–Hochberg (BH)-adjusted p-value < 0.05). Importantly, the immune checkpoint markers CD274 and CTLA4 demonstrated significantly higher expression in SCC compared to ADC. In addition, SCC showed a higher infiltration of immune cells, including B and T cells, and cytotoxic cells. Higher activation of a variety of pathways was found in SCC samples including cytotoxicity, interferon signaling, metabolic stress, lymphoid compartment, hypoxia, PI3k-AKT, hedgehog signaling and Notch signaling pathways. Our findings show distinctive gene expression patterns, signaling pathway activations, and trends in immune cell infiltration between SCC and ADC in cervical cancer. This study underscores the heterogeneity within primary cervical cancer, emphasizing the potential benefits of subdividing these tumours based on histological and molecular differences.
Animal Models and Molecular Pathogenesis of Arrhythmogenic Cardiomyopathy Associated with Pathogenic Variants in Intercalated Disc Genes
Authors: Vencato Sara , Romanato Chiara , Rampazzo Alessandra , Calore Martina
Published: June 2024

Arrhythmogenic cardiomyopathy (ACM) is a rare genetic cardiac disease characterized by the progressive substitution of myocardium with fibro-fatty tissue. Clinically, ACM shows wide variability among patients; symptoms can include syncope and ventricular tachycardia but also sudden death, with the latter often being its sole manifestation. Approximately half of ACM patients have been found with variations in one or more genes encoding cardiac intercalated discs proteins; the most involved genes are plakophilin 2 (PKP2), desmoglein 2 (DSG2), and desmoplakin (DSP). Cardiac intercalated discs provide mechanical and electro-metabolic coupling among cardiomyocytes. Mechanical communication is guaranteed by the interaction of proteins of desmosomes and adheren junctions in the so-called area composita, whereas electro-metabolic coupling between adjacent cardiac cells depends on gap junctions. Although ACM has been first described almost thirty years ago, the pathogenic mechanism(s) leading to its development are still only partially known. Several studies with different animal models point to the involvement of the Wnt/β-catenin signaling in combination with the Hippo pathway. Here, we present an overview about the existing murine models of ACM harboring variants in intercalated disc components with a particular focus on the underlying pathogenic mechanisms. Prospectively, mechanistic insights into the disease pathogenesis will lead to the development of effective targeted therapies for ACM.
Does Cell-Type-Specific Silencing of Monoamine Oxidase B Interfere with the Development of Right Ventricle (RV) Hypertrophy or Right Ventricle Failure in Pulmonary Hypertension?
Authors: Brosinsky Paulin , Heger Jacqueline , Sydykov Akylbek , Weiss Astrid , Klatt Stephan , Czech Laureen , Kraut Simone , Schermuly Ralph Theo , Schlüter Klaus-Dieter , Schulz Rainer
Published: June 2024

Increased mitochondrial reactive oxygen species (ROS) formation is important for the development of right ventricular (RV) hypertrophy (RVH) and failure (RVF) during pulmonary hypertension (PH). ROS molecules are produced in different compartments within the cell, with mitochondria known to produce the strongest ROS signal. Among ROS-forming mitochondrial proteins, outer-mitochondrial-membrane-located monoamine oxidases (MAOs, type A or B) are capable of degrading neurotransmitters, thereby producing large amounts of ROS. In mice, MAO-B is the dominant isoform, which is present in almost all cell types within the heart. We analyzed the effect of an inducible cardiomyocyte-specific knockout of MAO-B (cmMAO-B KO) for the development of RVH and RVF in mice. Right ventricular hypertrophy was induced by pulmonary artery banding (PAB). RV dimensions and function were measured through echocardiography. ROS production (dihydroethidium staining), protein kinase activity (PamStation device), and systemic hemodynamics (in vivo catheterization) were assessed. A significant decrease in ROS formation was measured in cmMAO-B KO mice during PAB compared to Cre-negative littermates, which was associated with reduced activity of protein kinases involved in hypertrophic growth. In contrast to littermates in which the RV was dilated and hypertrophied following PAB, RV dimensions were unaffected in response to PAB in cmMAO-B KO mice, and no decline in RV systolic function otherwise seen in littermates during PAB was measured in cmMAO-B KO mice. In conclusion, cmMAO-B KO mice are protected against RV dilatation, hypertrophy, and dysfunction following RV pressure overload compared to littermates. These results support the hypothesis that cmMAO-B is a key player in causing RV hypertrophy and failure during PH.
Rodent Models of Alzheimer’s Disease: Past Misconceptions and Future Prospects
Authors: Collins Helen M. , Greenfield Susan
Published: June 2024

Alzheimer’s disease (AD) is a progressive neurodegenerative disease with no effective treatments, not least due to the lack of authentic animal models. Typically, rodent models recapitulate the effects but not causes of AD, such as cholinergic neuron loss: lesioning of cholinergic neurons mimics the cognitive decline reminiscent of AD but not its neuropathology. Alternative models rely on the overexpression of genes associated with familial AD, such as amyloid precursor protein, or have genetically amplified expression of mutant tau. Yet transgenic rodent models poorly replicate the neuropathogenesis and protein overexpression patterns of sporadic AD. Seeding rodents with amyloid or tau facilitates the formation of these pathologies but cannot account for their initial accumulation. Intracerebral infusion of proinflammatory agents offer an alternative model, but these fail to replicate the cause of AD. A novel model is therefore needed, perhaps similar to those used for Parkinson’s disease, namely adult wildtype rodents with neuron-specific (dopaminergic) lesions within the same vulnerable brainstem nuclei, ‘the isodendritic core’, which are the first to degenerate in AD. Site-selective targeting of these nuclei in adult rodents may recapitulate the initial neurodegenerative processes in AD to faithfully mimic its pathogenesis and progression, ultimately leading to presymptomatic biomarkers and preventative therapies.
Exploring the Structure and Substance Metabolism of a Medicago sativa L. Stem Base
Authors: Gao Qian , Wang Kun , Huang Jing , Dou Pengpeng , Miao Zhengzhou
Published: June 2024

The stem base of alfalfa is a critical part for its overwintering, regeneration, and yield. To better understand the specificity and importance of the stem base, we analyzed the structure, metabolic substances, and transcriptome of the stem base using anatomical techniques, ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS), and RNA sequencing (RNA-seq), and compared it with stems and roots. The anatomical structure shows that the ratio of xylem to phloem changes at the base of the stem. A total of 801 compounds involved in 91 metabolic pathways were identified from the broadly targeted metabolome. Transcriptome analysis revealed 4974 differentially expressed genes (DEGs) at the stem base compared to the stem, and 5503 DEGs compared to the root. Comprehensive analyses of differentially accumulated compounds (DACs) and DEGs, in the stem base vs. stem, identified 10 valuable pathways, including plant hormone signal transduction, zeatin biosynthesis, α-Linolenic acid metabolism, histidine metabolism, carbon metabolism, carbon fixation in photosynthetic organisms, pentose phosphate pathway, galactose metabolism, and fructose and mannose metabolism. The pathways of plant hormone signal transduction and carbon metabolism were also identified by comparing the stem base with the roots. Taken together, the stem base of alfalfa is the transition region between the stem and root in morphology; in terms of material metabolism, its growth, development, and function are regulated through hormones and sugars.
TATA-Binding Protein-Based Virtual Screening of FDA Drugs Identified New Anti-Giardiasis Agents
Authors: Gaona-López Carlos , Méndez-Álvarez Domingo , Moreno-Rodríguez Adriana , Bautista-Martínez Juan Luis , De Fuentes-Vicente José Antonio , Nogueda-Torres Benjamín , García-Torres Itzhel , López-Velázquez Gabriel , Rivera Gildardo
Published: June 2024

Parasitic diseases, predominantly prevalent in developing countries, are increasingly spreading to high-income nations due to shifting migration patterns. The World Health Organization (WHO) estimates approximately 300 million annual cases of giardiasis. The emergence of drug resistance and associated side effects necessitates urgent research to address this growing health concern. In this study, we evaluated over eleven thousand pharmacological compounds sourced from the FDA database to assess their impact on the TATA-binding protein (TBP) of the early diverging protist Giardia lamblia, which holds medical significance. We identified a selection of potential pharmacological compounds for combating this parasitic disease through in silico analysis, employing molecular modeling techniques such as homology modeling, molecular docking, and molecular dynamics simulations. Notably, our findings highlight compounds DB07352 and DB08399 as promising candidates for inhibiting the TBP of Giardia lamblia. Also, these compounds and DB15584 demonstrated high efficacy against trophozoites in vitro. In summary, this study identifies compounds with the potential to combat giardiasis, offering the prospect of specific therapies and providing a robust foundation for future research.
The MicroRNAs in the Pathophysiology of Osteoporosis
Authors: Trojniak Julia , Sendera Anna , Banaś-Ząbczyk Agnieszka , Kopańska Marta
Published: June 2024

Globally, osteoporosis is the most common systemic skeletal disease. There are many factors that influence osteoporosis’ development and progression. During the pathogenesis of this disease, bone turnover is imbalanced between resorption and the formation of bone tissue. A growing interest has been devoted to the role that microRNA (miRNA) plays in osteoporosis regulation. A microRNA (miRNA) is a group of small single-stranded RNA molecules involved in regulating gene expression in eukaryotic organisms. As microRNAs (miRNAs) are key regulators of gene expression and can modulate processes related to bone metabolism, they have become increasingly important for studying osteoporosis pathogenesis. The available research suggests that miRNAs play an important role in regulating processes associated with bone metabolism, especially by influencing bone resorption and synthesis. Furthermore, microRNAs can also serve as potential therapeutic targets for osteoporosis, besides being a rapid and specific biomarker.
A Comparison of Mixed and Partial Membership Diagnostic Classification Models with Multidimensional Item Response Models
Authors: Robitzsch  Alexander
Published: June 2024

Diagnostic classification models (DCM) are latent structure models with discrete multivariate latent variables. Recently, extensions of DCMs to mixed membership have been proposed. In this article, ordinary DCMs, mixed and partial membership models, and multidimensional item response theory (IRT) models are compared through analytical derivations, three example datasets, and a simulation study. It is concluded that partial membership DCMs are similar, if not structurally equivalent, to sufficiently complex multidimensional IRT models.
A Survey of Text-Matching Techniques
Authors: Jiang Peng , Cai Xiaodong
Published: June 2024

Text matching, as a core technology of natural language processing, plays a key role in tasks such as question-and-answer systems and information retrieval. In recent years, the development of neural networks, attention mechanisms, and large-scale language models has significantly contributed to the advancement of text-matching technology. However, the rapid development of the field also poses challenges in fully understanding the overall impact of these technological improvements. This paper aims to provide a concise, yet in-depth, overview of the field of text matching, sorting out the main ideas, problems, and solutions for text-matching methods based on statistical methods and neural networks, as well as delving into matching methods based on large-scale language models, and discussing the related configurations, API applications, datasets, and evaluation methods. In addition, this paper outlines the applications and classifications of text matching in specific domains and discusses the current open problems that are being faced and future research directions, to provide useful references for further developments in the field.
Storing up Treasures: Storage Potential of Macrolophus pygmaeus (Hemiptera: Heteroptera: Miridae) Nymphs for Application in Biological Control
Authors: Pazyuk Irina M. , Dolgovskaya Margarita Y. , Reznik Sergey Y. , Musolin Dmitrii L.
Published: June 2024

Long-term storage is an important component of insect mass-rearing systems, prolonging the shelf life of biocontrol agents during a low-demand period or a temporary lack of suitable food. Macrolophus pygmaeus is a predatory heteropteran, mass-reared and widely used for the biological control of arthropod pests in greenhouses. With the aim to determine the optimal conditions and acceptable duration of cold storage, we evaluated the impact of different periods of storage of fed and starved third instar nymphs of M. pygmaeus at different temperatures on nymphal survival, adult emergence, and female fecundity. Four storage temperatures (3, 6, 9, and 12 °C) were tested. The longevity of starved nymphs decreased with an increase in the storage temperature, with a maximum of about 40 days at 3 °C, whereas the longest lifetime of nymphs fed on eggs of the grain moth Sitotroga cerealella (about 150 days) was observed at 9 °C. Further experiments demonstrated that the third instar nymphs of M. pygmaeus fed with eggs of the grain moth can be stored at 9 °C for 30 days, with a moderate (10–20%) decrease in survival and fecundity, whereas the survival of starved nymphs decreased by half after 10 days of storage at 3 °C.