bluuebunny/crossref_metadata_2025 huggingface dataset:
Sexuality, sexual expression in long-term care and the law
Authors: Aziz Victor M. , Slack Philip
Published: January 2021

Balancing risk and safety in long-term care settings can be challenging while providing and respecting patients’/residents’ needs and rights in terms of sexual expression. We look at factors affecting the expression of sexuality, including staff attitudes and reactions, and the lack of policies governing sexuality. We review the various statute and case law, other legislative and quasi-legal provisions governing sexuality and sexual expression. Finally, we consider the need for clear policies and training for both staff and family members.
Isotyping and Semi-Quantitation of Monkey Anti-Drug Antibodies by Immunocapture Liquid Chromatography-Mass Spectrometry
Authors: Huang Xiaoxiao , Xu Xiaobin , Partridge Michael A. , Chen Jihua , Koehler-Stec Ellen , Sumner Giane , Qiu Haibo , Torri Albert , Li Ning
Published: January 2021

There is an urgent demand to develop new technologies to characterize immunogenicity to biotherapeutics. Here, we developed an immunocapture LC-MS assay to isotype and semi-quantify monkey anti-drug antibodies (ADAs) to fully human monoclonal antibody (mAb) drugs. ADAs were isolated from serum samples using an immunocapture step with the Fab of the full-length mAb cross-linked to magnetic beads to minimize matrix interference. A positive monoclonal antibody control against the human immunoglobulin kappa light chain was used as a calibration standard for ADA quantitation. The final LC-MS method contains 17 multiple reaction monitoring (MRM) transitions and an optimized 15-min LC method. The results suggested that IgG1 was the most abundant isotype in ADA-positive samples. IgG2 and IgG4 were identified at lower levels, whereas IgG3 and IgA levels were only observed at very minor levels. In addition, levels of total ADA measured by the LC-MS assay were comparable to results obtained using a traditional ligand binding assay (LBA). The LC-MS ADA assay enabled rapid immunogenicity assessment with additional isotype information that LBAs cannot provide.
Subcutaneous Injection Performance in Yucatan Miniature Pigs with and without Human Hyaluronidase and Auto-injector Tolerability in Humans
Authors: Shi Galen H. , Connor Robert J. , Collins David S. , Kang David W.
Published: January 2021

Recombinant human hyaluronidase PH20 (rHuPH20) facilitates subcutaneous (SC) delivery of co-administered therapeutic agents by locally and transiently degrading hyaluronan in the SC space, and can be administered with therapeutics using a variety of devices. Two SC delivery studies were carried out to assess auto-injector (AI) performance, each in 18 Yucatan miniature pigs. Abdominal injections were administered using three auto-injectors of 1 mL (AI1) and 2 mL (AI2 and sAI2) with different injection speeds and depths (5.5–7.5 mm) and two pre-filled syringe (PFS) devices of 1 and 2 mL. The injection included a placebo buffer with and without rHuPH20 to evaluate the effect of rHuPH20 on SC injection performance. The feasibility of using similar devices to deliver a placebo buffer in humans was investigated. rHuPH20 was not studied in humans. In miniature pigs, postinjection swelling was evident for most PFS/AI injections, particularly 2 mL. Swelling heights and back leakage were typically lower with rHuPH20 co-administration versus placebo for most device configurations (1 or 2 mL PFS or AI). Auto-injections with versus without rHuPH20 also resulted in reduced swelling firmness and faster swelling resolution over time. Slow injections with rHuPH20 had shorter and more consistent injection time versus placebo. In humans, minimal injection site swelling and negligible back leakage were observed for 2-mL injections of placebo, while more erythema was observed in humans versus miniature pigs. Even at high delivery rates with PFS or AI, the addition of rHuPH20 resulted in improved SC injection performance versus placebo in miniature pigs.
JUSTIFICATION OF MANAGEMENT SOLUTIONS FOR THE DELIVERY OF DIESEL FUEL TO THE MINES
Authors: Olizarenko Vladimir , Allaberdin Azamat , Vazhdaev Konstantin
Published: January 2021

The development of copper-pyrite deposits in the southern Urals is characterized by an increase in depth and, consequently, a complication of mining and geological conditions. As a result, there is a significant increase in the volume of work on the sinking of mine workings, delivery, and rollback of the extracted ore mass. Justification of management decisions on the delivery of diesel fuel to the mine is executed to find the optimal model of the technological scheme of delivery of diesel fuel (diesel fuel) and other fuels and lubricants (further – fuels and lubricants) in the deep horizons of the underground mine (in the sequel - in the mine) with the development of recommendations on designing an optimal model of method of delivery. The latter is achieved by comparing the competitive models of delivery of DT to the mine: fuel tankers (hereinafter-TK) on an inclined ramp, through a pipeline laid in a cased well and in specially equipped mine trolleys and their combinations with a reasonable choice of the optimal model of the technological scheme. According to the results of the research developed methods of rational organization and management of the movement of diesel fuel based on material flows on the principles of logistics concepts, ensuring the formation of counter-flows model logistic schemes. From the above studies, it follows that the delivery of DT to the mine in special trolleys is optimal with minimal time and cost of delivery to deep horizons Gaiskiy underground mine.
The burden of dehisced wounds in the community: using early results from a multi-centre service evaluation to propose a standard of care to improve patient outcomes and safeguard woundcare budgets
Authors: Hughes Jacqui , Costello Mary , Belshaw Mandy , Horton Helen , Styche Tim
Published: January 2021

Non-healing wounds and their associated costs present a significant challenge to wound care services. Dehisced wounds are often overlooked, with a lack of true understanding regarding the additional burden this represents. As a consequence, there is a lack of evidence around what is deemed gold standard care, with no published pathways to support clinical practice. A 34-patient data set was captured and analysed as part of an evaluation of hard-to-heal wounds. Patients with wounds meeting specific criteria followed a hard-to-heal wound pathway that facilitated single use negative pressure wound therapy. Implementation of this pathway helped to achieve healing for 18 out of 34 (52.9%) wounds over a 12-week period, subsequently reducing the overall wound care spend from £76 828 to £60 251 for this group. A notably improved response was seen in patients who had a chronic wound of less than 6 months in duration, indicating the importance of earlier intervention and the positive impact this can have on the patient experience and associated health economic outcomes. This service evaluation supports the use of single use negative pressure wound therapy for non-progressing dehisced surgical wounds as part of an indication-led pathway.
Optimal Bezier curve transition for low-loss ultra-compact S-bends
Authors: Yi Dan , Zhang Yaojing , Tsang Hon Ki
Published: January 2021

S-bends with a widening of the width at the mid-bend and a Bezier curve transition are proposed and demonstrated for low-loss S-bends. The increased optical confinement and reduced transition loss enable low insertion loss (IL) and compact S-bends with longitudinal offsets as small as 2.5 µm and a wide operating bandwidth (

∼

100

n
m

) on a 220 nm thick silicon-on-insulator platform. The simulation results show ILs less than (0.22, 0.20, 0.20, 0.20) dB in the wavelength range of (1.5–1.6) µm, while the minimum ILs are (0.13, 0.13, 0.15, 0.16) dB for lateral offsets of (3, 6, 9, 12) µm. The experimental results show that ILs remain less than (0.41, 0.38, 0.36, 0.39) dB for the mid-bend widening Bezier (MWB) S-bends.
The risks of using the chi-square periodogram to estimate the period of biological rhythms
Authors: Tackenberg Michael C. , Hughey Jacob J.
Published: January 2021

The chi-square periodogram (CSP), developed over 40 years ago, continues to be one of the most popular methods to estimate the period of circadian (circa 24-h) rhythms. Previous work has indicated the CSP is sometimes less accurate than other methods, but understanding of why and under what conditions remains incomplete. Using simulated rhythmic time-courses, we found that the CSP is prone to underestimating the period in a manner that depends on the true period and the length of the time-course. This underestimation bias is most severe in short time-courses (e.g., 3 days), but is also visible in longer simulated time-courses (e.g., 12 days) and in experimental time-courses of mouse wheel-running and ex vivo bioluminescence. We traced the source of the bias to discontinuities in the periodogram that are related to the number of time-points the CSP uses to calculate the observed variance for a given test period. By revising the calculation to avoid discontinuities, we developed a new version, the greedy CSP, that shows reduced bias and improved accuracy. Nonetheless, even the greedy CSP tended to be less accurate on our simulated time-courses than an alternative method, namely the Lomb-Scargle periodogram. Thus, although our study describes a major improvement to a classic method, it also suggests that users should generally avoid the CSP when estimating the period of biological rhythms.
A unique group of scabies mite pseudoproteases promotes cutaneous blood coagulation and delays plasmin-induced fibrinolysis
Authors: Fernando Deepani D. , Reynolds Simone L. , Hartel Gunter , Cribier Bernard , Ortonne Nicolas , Jones Malcolm K. , Fischer Katja
Published: January 2021

Background

Scabies, a highly contagious skin disease affecting more than 200 million people worldwide at any time, is caused by the parasitic mite Sarcoptes scabiei. In the absence of molecular markers, diagnosis requires experience making surveillance and control challenging. Superficial microthrombi in the absence of vasculitis in scabies-affected skin are a recognised, yet unexplained histopathological differential of scabies infection. This study demonstrates that a family of Scabies Mite Inactivated Cysteine Protease Paralogues (SMIPP-Cs) excreted by the mites plays a role in formation of scabies-induced superficial microthrombi.

Methodology/Principal findings

A series of in vitro and ex vivo experiments involving two representative recombinant SMIPP-Cs was carried out. In the presence of SMIPP-Cs, the thrombin clotting time (TCT), fibrin formation and plasmin induced fibrinolysis were monitored in vitro. The ultrastructure of the SMIPP-C—modulated fibrin was analysed by Scanning Electron Microscopy (SEM). Immuno-histological analyses were performed ex vivo, to localise the SMIPP-C proteins within scabies infected skin biopsies. SMIPP-Cs displayed pro-coagulant properties. They bound calcium ions, reduced the thrombin clotting time, enhanced the fibrin formation rate and delayed plasmin-induced fibrinolysis. The SMIPP-Cs associated with fibrin clots during fibrinogen polymerisation and did not bind to preformed fibrin. Scanning electron microscopy revealed that the fibrin clots formed in the presence of SMIPP-Cs were aberrant and denser than normal fibrin clots. SMIPP-Cs were detected in microthrombi which are commonly seen in scabietic skin.

Conclusions/Significance

The SMIPP-Cs are the first scabies mite proteins found in sub-epidermal skin layers and their pro-coagulant properties promote superficial microthrombi formation in scabetic skin. Further research is needed to evaluate their potential as diagnostic or therapeutic target.
Effect of early oxygen therapy and antiviral treatment on disease progression in patients with COVID-19: A retrospective study of medical charts in China
Authors: Long Lu , Wu Liang , Chen Lang , Zhou Daixing , Wu Hongyu , Lu Donghao , Li Honglin , Duan Xiaoxia , Han Yutong , Li Xianzhi , Wang Qiuxia , Zhang Jing
Published: January 2021

Background

Until now, no antiviral treatment has been proven to be effective for the coronavirus disease 2019 (COVID-19). The timing of oxygen therapy was considered to have a great influence on the symptomatic relief of hypoxemia and seeking medical intervention, especially in situations with insufficient medical resources, but the evidence on the timing of oxygen therapy is limited.

Methods and findings

Medical charts review was carried out to collect the data of hospitalized patients with COVID-19 infection confirmed in Tongji hospital, Wuhan from 30th December 2019 to 8th March 2020. In this study, the appropriate timing of oxygen therapy and risk factors associated with severe and fatal illness were identified and the effectiveness of antivirus on disease progression was assessed. Among 1362 patients, the prevalence of hypoxia symptoms was significantly higher in those patients with severe and fatal illness than in those with less severe disease. The onset of hypoxia symptoms was most common in the second to third week after symptom onset, and patients with critical and fatal illness experienced these symptoms earlier than those with mild and severe illness. In multivariable analyses, the risk of death increased significantly when oxygen therapy was started more than 2 days after hypoxia symptoms onset among critical patients (OR, 1.92; 95%CI, 1.20 to 3.10). Compared to the critically ill patients without IFN-a, the patients who were treated with IFN-a had a lower mortality (OR, 0.60; 95%CI, 0.39 to 0.91).

Conclusions

Early initiation of oxygen therapy was associated with lower mortality among critical patients. This study highlighted the importance of early oxygen therapy after the onset of hypoxia symptoms. Our results also lend support to potentially beneficial effects of IFNα on critical illness.
Gene-environment interaction in molar-incisor hypomineralization
Authors: Bezamat Mariana , Souza Juliana F. , Silva Fernanda M. F. , Corrêa Emilly G. , Fatturi Aluhe L. , Brancher João A. , Carvalho Flávia M. , Cavallari Tayla , Bertolazo Laís , Machado-Souza Cleber , Koruyucu Mine , Bayram Merve , Racic Andrea , Harrison Benjamin M. , Sweat Yan Y. , Letra Ariadne , Studen-Pavlovich Deborah , Seymen Figen , Amendt Brad , Werneck Renata I. , Costa Marcelo C. , Modesto Adriana , Vieira Alexandre R.
Published: January 2021

Molar incisor hypomineralization (MIH) is an enamel condition characterized by lesions ranging in color from white to brown which present rapid caries progression, and mainly affects permanent first molars and incisors. These enamel defects usually occur when there are disturbances during the mineralization or maturation stage of amelogenesis. Both genetic and environmental factors have been suggested to play roles in MIH’s development, but no conclusive risk factors have shown the source of the disease. During head and neck development, the interferon regulatory factor 6 (IRF6) gene is involved in the structure formation of the oral and maxillofacial regions, and the transforming growth factor alpha (TGFA) is an essential cell regulator, acting during proliferation, differentiation, migration and apoptosis. In this present study, it was hypothesized that these genes interact and contribute to predisposition of MIH. Environmental factors affecting children that were 3 years of age or older were also hypothesized to play a role in the disease etiology. Those factors included respiratory issues, malnutrition, food intolerance, infection of any sort and medication intake. A total of 1,065 salivary samples from four different cohorts were obtained, and DNA was extracted from each sample and genotyped for nine different single nucleotide polymorphisms. Association tests and logistic regression implemented in PLINK were used for analyses. A potential interaction betweenTGFArs930655 with all markers tested in the cohort from Turkey was identified. These interactions were not identified in the remaining cohorts. Associations (p<0.05) between the use of medication after three years of age and MIH were also found, suggesting that conditions acquired at the age children start to socialize might contribute to the development of MIH.
Words describing feelings about death: A comparison of sentiment for self and others and changes over time
Authors: Miller-Lewis Lauren R. , Lewis Trent W. , Tieman Jennifer , Rawlings Deb , Parker Deborah , Sanderson Christine R.
Published: January 2021

Understanding public attitudes towards death is needed to inform health policies to foster community death awareness and preparedness. Linguistic sentiment analysis of how people describe their feelings about death can add to knowledge gained from traditional self-reports. This study provided the first description of emotive attitudes expressed towards death utilising textual sentiment analysis for the dimensions of valence, arousal and dominance. A linguistic lexicon of sentiment norms was applied to activities conducted in an online course for the general-public designed to generate discussion about death. We analysed the sentiment of words people chose to describe feelings about death, for themselves, for perceptions of the feelings of ‘others’, and for longitudinal changes over the time-period of exposure to a course about death (n= 1491). The results demonstrated that sadness pervades affective responses to death, and that inevitability, peace, and fear were also frequent reactions. However, words chosen to represent perceptions of others’ feelings towards death suggested that participants perceived others as feeling more negative about death than they do themselves. Analysis of valence, arousal and dominance dimensions of sentiment pre-to-post course participation demonstrated that participants chose significantly happier (more positive) valence words, less arousing (calmer) words, and more dominant (in-control) words to express their feelings about death by the course end. This suggests that the course may have been helpful in participants becoming more emotionally accepting in their feelings and attitude towards death. Furthermore, the change over time appeared greater for younger participants, who showed more increase in the dominance (power/control) and pleasantness (valence) in words chosen at course completion. Sentiment analysis of words to describe death usefully extended our understanding of community death attitudes and emotions. Future application of sentiment analysis to other related areas of health policy interest such as attitudes towards Advance Care Planning and palliative care may prove fruitful.
Long-term effects of catastrophic wind on southern US coastal forests: Lessons from a major hurricane
Authors: Sharma Ajay , Ojha Santosh K. , Dimov Luben D. , Vogel Jason G. , Nowak Jarek
Published: January 2021

Threats posed by windstorms are an increasing concern to forest managers in the southern United States (US). Studies suggest that the southern US will experience an increase in the occurrence as well as the intensity of windstorms, such as hurricanes, in the future. However, forest managers may have difficulty preparing for this future because there is limited understanding of how windstorms affect the structure and composition of forests over the long term. In this study, we evaluated the impacts of Hurricane Ivan, which made landfall in September 2004 near Gulf Shore, Alabama, impacting forests in the western Florida Panhandle and southwestern Alabama. We acquired the United States Department of Agriculture Forest Inventory and Analysis (FIA) plot data available for the period from 2002 to 2018 for the Ivan-affected area and classified the plots into 4 categories: (1). ND (No Disturbance), (2). NDBH (No Disturbance but Harvested), (3). ID (Disturbance caused by Hurricane Ivan), and (4). IDAH (Disturbance caused by Hurricane Ivan and Harvested). The plots that were damaged by Hurricane Ivan (ID and IDAH plots) had significantly (α = 0.05) (1) higher basal area, (2) higher quadratic mean diameter and height, (3) more diverse tree species composition (species richness and Shannon diversity index), (4) denser stocking of seedling and saplings, (5) lower proportion of dead trees or saplings, and (6) higher live aboveground biomass than the plots that were not damaged by the hurricane (ND and NDBH plots). Diverse stands were not necessarily more windstorm resistant. Species diversity in the overstory may not improve forest resistance to hurricane damage but may improve its resilience following the hurricane. The study suggests that managing stand structure through density management and stand improvement could be critical to windstorm resilience and resistance in the southern US forests.
Vertical distribution of prokaryotes communities and predicted metabolic pathways in New Zealand wetlands, and potential for environmental DNA indicators of wetland condition
Authors: Wood Jamie R. , Burge Olivia R. , Bolstridge Nic , Bonner Karen , Clarkson Beverley , Cole Theresa L. , Davis Carina , Fergus Alex , King Perēri , McKeown Michelle M. , Morse Chris , Richardson Sarah J. , Robertson Hugh , Wilmshurst Janet M.
Published: January 2021

Globally, wetlands are in decline due to anthropogenic modification and climate change. Knowledge about the spatial distribution of biodiversity and biological processes within wetlands provides essential baseline data for predicting and mitigating the effects of present and future environmental change on these critical ecosystems. To explore the potential for environmental DNA (eDNA) to provide such insights, we used 16S rRNA metabarcoding to characterise prokaryote communities and predict the distribution of prokaryote metabolic pathways in peats and sediments up to 4m below the surface across seven New Zealand wetlands. Our results reveal distinct vertical structuring of prokaryote communities and metabolic pathways in these wetlands. We also find evidence for differences in the relative abundance of certain metabolic pathways that may correspond to the degree of anthropogenic modification the wetlands have experienced. These patterns, specifically those for pathways related to aerobic respiration and the carbon cycle, can be explained predominantly by the expected effects of wetland drainage. Our study demonstrates that eDNA has the potential to be an important new tool for the assessment and monitoring of wetland health.
When gravity is not where it should be: How perceived orientation affects visual self-motion processing
Authors: McManus Meaghan , Harris Laurence R.
Published: January 2021

Human perception is based on expectations. We expect visual upright and gravity upright, sensed through vision, vestibular and other sensory systems, to agree. Equally, we expect that visual and vestibular information about self-motion will correspond. What happens when these assumptions are violated? Tilting a person from upright so that gravity is not where it should be impacts both visually induced self-motion (vection) and the perception of upright. How might the two be connected? Using virtual reality, we varied the strength of visual orientation cues, and hence the probability of participants experiencing a visual reorientation illusion (VRI) in which visual cues to orientation dominate gravity, using an oriented corridor and a starfield while also varying head-on-trunk orientation and body posture. The effectiveness of the optic flow in simulating self-motion was assessed by how much visual motion was required to evoke the perception that the participant had reached the position of a previously presented target. VRI was assessed by questionnaire When participants reported higher levels of VRI they also required less visual motion to evoke the sense of traveling through a given distance, regardless of head or body posture, or the type of visual environment. We conclude that experiencing a VRI, in which visual-vestibular conflict is resolved and the direction of upright is reinterpreted, affects the effectiveness of optic flow at simulating motion through the environment. Therefore, any apparent effect of head or body posture or type of environment are largely indirect effects related instead, to the level of VRI experienced by the observer. We discuss potential mechanisms for this such as reinterpreting gravity information or altering the weighting of orientation cues.
Awareness and practice of medical waste management among healthcare providers in National Referral Hospital
Authors: Letho Zimba , Yangdon Tshering , Lhamo Chhimi , Limbu Chandra Bdr , Yoezer Sonam , Jamtsho Thinley , Chhetri Puja , Tshering Dawa
Published: January 2021

Introduction

The management and treatment of Medical Waste (MW) are of great concern owing to its potential hazard to human health and the environment, particularly in developing countries. In Bhutan, although guidelines exist on the prevention and management of wastes, the implementation is still hampered by technological, economic, social difficulties and inadequate training of staff responsible for handling these waste. The study aimed at assessing the awareness and practice of medical waste management among health care providers and support staff at the National Referral Hospital and its compliance with the existing National guidelines and policies.

Materials and methods

An observational cross-sectional study was conducted from March to April 2019. Three research instruments were developed and used; (i) Demographic questionnaire, (ii) Awareness questions, and (iii) the Observational checklist. The data was coded and double entered into Epi data version 3.1 and SPSS version 18 was used for analysis. Descriptive statistics were used to present the findings of the study.

Results

The majority of the respondents were female (54.1%) with a mean age of 32.2 (±7.67) years, most of whom have not received any waste management related training/education (56.8%). About 74.4% are aware of medical waste management and 98.2% are aware on the importance of using proper personal protective equipment. Only 37.6% knew about the maximum time limit for medical waste to be kept in hospital premises is 48 hours. About 61.3% of the observed units/wards/departments correctly segregated the waste in accordance to the national guidelines. However, half of the Hospital wastes are not being correctly transported based on correct segregation process with 58% of waste not segregated into infectious and general wastes.

Conclusion

The awareness and practice of medical waste management among healthcare workers is often limited with inadequate sensitization and lack of proper implementation of the existing National guidelines at the study site. Therefore, timely and effective monitoring is required with regular training for healthcare workers and support staff. Furthermore, strengthening the waste management system at National Referral Hospital would provide beneficial impact in enhancing safety measures of patients.
Taxonomic revision of Madagascan species of the Pheidole fervens species-group (Hymenoptera, Formicidae)
Authors: Salata Sebastian , Fisher Brian L.
Published: January 2021

Madagascar, one of the top megadiversity regions, hosts one of the highest numbers of endemic and threatened organisms on earth. One of the most spectacular examples of ant radiation on the island has occurred in the hyperdiverse genusPheidole. To this date, there are 117 described MadagascanPheidoledivided into 16 species-groups, and 97% of them are endemic to the island. Only two of these species-groups contain widely distributed invasive species in addition to native, endemic taxa:megacephala, andfervensspecies-groups. Here we revise thefervensspecies-group and discuss updated distribution records of its introduced members on Madagascar. We recognize six species belonging to this group, including five new to science:Pheidole ampangabesp. nov.,P.arivosp. nov.,P.comosasp. nov.,P.indicaMayr,P.mamirapiratrasp. nov., andP.menasp. nov. Detailed descriptions are supplemented with measurements, diagnoses, identification key, high-resolution images for major and minor worker, and comments on distribution and biology.
Evaluating survival in subjects with astrocytic brain tumors by dynamic susceptibility-weighted perfusion MR imaging
Authors: White Matthew L. , Zhang Yan , Kazmi Syed A. Jaffar , Aizenberg Michele , Shonka Nicole , Yu Fang , Appiah Adams Kusi
Published: January 2021

Purpose

Studies have evaluated the application of perfusion MR for predicting survival in patients with astrocytic brain tumors, but few of them statistically adjust their results to reflect the impact of the variability of treatment administered in the patients. Our aim was to analyze the association between the perfusion values and overall survival time, with adjustment for various clinical factors, including initial treatments and follow-up treatments.

Materials and methods

This study consisted of 51 patients with astrocytic brain tumors who underwent perfusion-weighted MRI with MultiHance® at a dose of 0.1 mmol/kg prior to initial surgery. We measured the mean rCBV, the 5% & 10% maximum rCBV, and the variation of rCBV in the tumors. Comparisons were made between patients with and without 2-year survival using two-sample t-test or Wilcoxon rank-sum test for the continuous data, or chi-square and Fisher exact tests for categorical data. The multivariate cox-proportional hazard regression was fit to evaluate the association between rCBV and overall survival time, with adjustment for clinical factors.

Results

Patients who survived less than 2 years after diagnosis had a higher mean and maximum rCBV and a larger variation of rCBV. After adjusting for clinical factors including therapeutic measures, we found no significant association of overall survival time within 2 years with any of these rCBV values.

Conclusions

Although patients who survived less than 2 years had a higher mean and maximum rCBV and a larger variation of rCBV, rCBV itself may not be used independently for predicting 2-year survival of patients with astrocytic brain tumors.
COVID-19 and mental health deterioration by ethnicity and gender in the UK
Authors: Proto Eugenio , Quintana-Domeque Climent
Published: January 2021

We use the UK Household Longitudinal Study and compare pre-COVID-19 pandemic (2017-2019) and during-COVID-19 pandemic data (April 2020) for the same group of individuals to assess and quantify changes in mental health as measured by changes in the GHQ-12 (General Health Questionnaire), among ethnic groups in the UK. We confirm the previously documented average deterioration in mental health for the whole sample of individuals interviewed before and during the COVID-19 pandemic. In addition, we find that the average increase in mental distress varies by ethnicity and gender. Both women –regardless of their ethnicity– and Black, Asian, and minority ethnic (BAME) men experienced a higher average increase in mental distress than White British men, so that the gender gap in mental health increases only among White British individuals. These ethnic-gender specific changes in mental health persist after controlling for demographic and socioeconomic characteristics. Finally, we find some evidence that, among men, Bangladeshi, Indian and Pakistani individuals have experienced the highest average increase in mental distress with respect to White British men.
Field-based body temperatures reveal behavioral thermoregulation strategies of the Atlantic marsh fiddler crab Minuca pugnax
Authors: Hews Sarah , Allen Zahkeyah , Baxter Adrienne , Rich Jacquline , Sheikh Zahida , Taylor Kayla , Wu Jenny , Zakoul Heidi , Brodie Renae
Published: January 2021

Behavioral thermoregulation is an important defense against the negative impacts of climate change for ectotherms. In this study we examined the use of burrows by a common intertidal crab, Minuca pugnax, to control body temperature. To understand how body temperatures respond to changes in the surface temperature and explore how efficiently crabs exploit the cooling potential of burrows to thermoregulate, we measured body, surface, and burrow temperatures during low tide on Sapelo Island, GA in March, May, August, and September of 2019. We found that an increase in 1°C in the surface temperature led to a 0.70-0.71°C increase in body temperature for females and an increase in 0.75-0.77°C in body temperature for males. Body temperatures of small females were 0.3°C warmer than large females for the same surface temperature. Female crabs used burrows more efficiently for thermoregulation compared to the males. Specifically, an increase of 1°C in the cooling capacity (the difference between the burrow temperature and the surface temperature) led to an increase of 0.42-0.50°C for females and 0.34-0.35°C for males in the thermoregulation capacity (the difference between body temperature and surface temperature). The body temperature that crabs began to use burrows to thermoregulate was estimated to be around 24°C, which is far below the critical body temperatures that could lead to death. Many crabs experience body temperatures of 24°C early in the reproductive season, several months before the hottest days of the year. Because the use of burrows involves fitness trade-offs, these results suggest that warming temperatures could begin to impact crabs far earlier in the year than expected.
Towards a comprehensive taxonomic revision of the Neotropical dung beetle subgenus Deltochilum (Deltohyboma) Lane, 1946 (Coleoptera: Scarabaeidae: Scarabaeinae): Division into species-groups
Authors: González-Alvarado Arturo , Vaz-de-Mello Fernando Z.
Published: January 2021

DeltochilumEschscholtz, 1822 is perhaps the most speciose genus of the tribe DeltochilinisensuTarasov & Dimitrov (2016) (Scarabaeidae: Scarabaeinae) and has been traditionally divided into eight subgenera. Among them, the subgenusDeltohybomaLane, 1946, is the most speciose with 47 species, five of which are described here (D.genierisp. nov.,D.gillisp. nov.,D.susanaesp. nov.,D.bolivariensissp. nov.andD.inesaesp. nov.), and at least 165 species still undescribed. Due to the large number of species, and for practical purposes, the subgenus is here divided into 19 species-groups, withD.inesaesp. nov.left asincertae sedis. This division into species-groups will help in the reliable identification of species and will aid in the completion of the revision of subgenus. This report is the first part of the taxonomic revision of the subgenusDeltohyboma; it is based on the examination of all type specimens and almost 9,800 specimens of which approximately 1,200, mostly males, had their genitalia studied. The 19 species-groups recognized here are based mainly on characters described for the first time forDeltohyboma, namely, the state of a) the anterior margin of the clypeus (between the clypeal teeth), b) the internal margin of hypomera, c) the ventral face of the protibia, d) the posterior margin of the metafemur, and e) several new characters resulting from the first detailed study of the aedeagus and the endophallus of the group. Diagnosis, description, geographic distribution, composition and identifications keys (for males as well as males and females) for the species-groups are provided.
Synergistic antibacterial effects of colistin in combination with aminoglycoside, carbapenems, cephalosporins, fluoroquinolones, tetracyclines, fosfomycin, and piperacillin on multidrug resistant Klebsiella pneumoniae isolates
Authors: Ontong Julalak C. , Ozioma Nwabor F. , Voravuthikunchai Supayang P. , Chusri Sarunyou
Published: January 2021

Multidrug resistant Enterobacterales have become a serious global health problem, with extended hospital stay and increased mortality. Antibiotic monotherapy has been reported ineffective against most drug resistant bacteria including Klebsiella pneumoniae, thus encouraging the use of multidrug therapies as an alternative antibacterial strategy. The present works assessed the antibacterial activity of colistin against K. pneumoniae isolates. Resistant isolates were tested against 16 conventional antibiotics alone and in combination with colistin. The results revealed that all colistin resistant isolates demonstrated multidrug resistance against the tested antibiotics except amikacin. At sub-inhibitory concentrations, combinations of colistin with amikacin, or fosfomycin showed synergism against 72.72% (8 of 11 isolates). Colistin with either of gentamicin, meropenem, cefoperazone, cefotaxime, ceftazidime, moxifloxacin, minocycline, or piperacillin exhibited synergism against 81.82% (9 of 11 isolates). Combinations of colistin with either of tobramycin or ciprofloxacin showed synergism against 45.45% (5 in 11 isolates), while combinations of colistin with imipenem or ceftolozane and tazobactam displayed 36.36% (4 of 11 isolates) and 63.64% (7 of 11 isolates) synergism. In addition, combinations of colistin with levofloxacin was synergistic against 90.91% (10 of 11 isolates). The results revealed that combinations of colistin with other antibiotics could effectively inhibit colistin resistant isolates of K. pneumoniae, and thus could be further explore for the treatment of multidrug resistant pathogens.
Using Fitbit data to examine factors that affect daily activity levels of college students
Authors: Wang Cheng , Lizardo Omar , Hachen David S.
Published: January 2021

To date, the effect of both fixed and time-varying individual, social, psychological, environmental, and behavioral characteristics on temporal growth trends in physical activity (PA) among younger individuals remains an under-studied topic. In this paper, we address this gap in previous work by examining how temporal growth trends in PA respond to changing social, environmental, and behavioral characteristics using a large sample of college students (N = 692) who participated in the NetHealth project at the University of Notre Dame and from which fine-grained longitudinal data on physical activity and social interaction were collected unobtrusively via the use of wearables for 637 days (August 16, 2015 to May 13, 2017). These data are augmented by periodic survey data on fixed sociodemographic and psychological variables. We estimate latent growth-curve models for daily activity status, steps, active minutes, and activity calories. We find evidence of both a generalized friendship paradox and a peer effect for PA, with the average PA level of study participants’ contacts being on average larger than their own, and with this average level exerting a statistically significant effect on individual PA levels. Notably, there was limited evidence of temporal growth in PA across the 637 days of observation with null temporal effects for three out of the four PA indicators, except for daily steps taken. Finally, we find that social, psychological, and behavioral factors (e.g., large network size, high extroversion levels, and more courses taken) are systematically associated with higher PA levels in this sample. Overall, our findings highlight the importance of social, environmental, and behavioral factors (such as peer networks and daily sociability) in modulating the dynamics of PA levels among college students.
Development and evaluation of a multiplex loop-mediated isothermal amplification (LAMP) assay for differentiation of Mycobacterium tuberculosis and non-tuberculosis mycobacterium in clinical samples
Authors: Kim Jeeyong , Park Borae G. , Lim Da Hye , Jang Woong Sik , Nam Jeonghun , Mihn Do-CiC , Lim Chae Seung
Published: January 2021

Introduction

The rapid and accurate diagnosis of tuberculosis (TB) is important to reduce morbidity and mortality rates and risk of transmission. Therefore, molecular detection methods such as a real-time PCR–based assay for Mycobacterium tuberculosis (MTB) have been commonly used for diagnosis of TB. Loop-mediated isothermal amplification (LAMP) assay was believed to be a simple, quick, and cost-effective isothermal nucleic acid amplification diagnostic test for infectious diseases. In this study, we designed an in-house multiplex LAMP assay for the differential detection of MTB and non-tuberculosis mycobacterium (NTM), and evaluated the assay using clinical samples.

Material and methods

For the multiplex LAMP assay, two sets of specific primers were designed: the first one was specific for IS6110 genes of MTB, and the second one was universal for rpoB genes of mycobacterium species including NTM. MTB was confirmed with a positive reaction with both primer sets, and NTM was identified with a positive reaction by only the second primer set without a MTB-specific reaction. Total 333 clinical samples were analyzed to evaluate the multiplex LAMP assay. Clinical samples were composed of 195 positive samples (72 MTB and 123NTM) and 138 negative samples. All samples were confirmed positivity or negativity by real-time PCR for MTB and NTM. Analytical sensitivity and specificity were evaluated for the multiplex LAMP assay in comparison with acid fast bacilli staining and the culture method.

Results

Of 123 NTM samples, 121 were identified as NTM and 72/72 MTB were identified as MTB by the multiplex LAMP assay. False negative reactions were seen only in two NTM positive samples with co-infection of Candida spp. All 138 negative samples were identified as negative for MTB and NTM. Analytical sensitivity of the multiplex LAMP assay was 100% (72/72) for MTB, and 98.4% (121/123) for NTM. And the specificity of assay was 100% (138/138) for all.

Conclusions

Our newly designed multiplex LAMP assay for MTB and NTM showed relatively good sensitivity in comparison with previously published data to detect isolated MTB. This multiplex LAMP assay is expected to become a useful tool for detecting and differentiating MTB from NTM rapidly at an acceptable sensitivity.
Screening accuracy of a 14-day smartphone ambulatory assessment of depression symptoms and mood dynamics in a general population sample: Comparison with the PHQ-9 depression screening
Authors: Burchert Sebastian , Kerber André , Zimmermann Johannes , Knaevelsrud Christine
Published: January 2021

Introduction

Major depression affects over 300 million people worldwide, but cases are often detected late or remain undetected. This increases the risk of symptom deterioration and chronification. Consequently, there is a high demand for low threshold but clinically sound approaches to depression detection. Recent studies show a great willingness among users of mobile health apps to assess daily depression symptoms. In this pilot study, we present a provisional validation of the depression screening app Moodpath. The app offers a 14-day ambulatory assessment (AA) of depression symptoms based on the ICD-10 criteria as well as ecologically momentary mood ratings that allow the study of short-term mood dynamics.

Materials and methods

N = 113 Moodpath users were selected through consecutive sampling and filled out the Patient Health Questionnaire (PHQ-9) after completing 14 days of AA with 3 question blocks (morning, midday, and evening) per day. The psychometric properties (sensitivity, specificity, accuracy) of the ambulatory Moodpath screening were assessed based on the retrospective PHQ-9 screening result. In addition, several indicators of mood dynamics (e.g. average, inertia, instability), were calculated and investigated for their individual and incremental predictive value using regression models.

Results

We found a strong linear relationship between the PHQ-9 score and the AA Moodpath depression score (r = .76, p < .001). The app-based screening demonstrated a high sensitivity (.879) and acceptable specificity (.745). Different indicators of mood dynamics covered substantial amounts of PHQ-9 variance, depending on the number of days with mood data that were included in the analyses.

Discussion

AA and PHQ-9 shared a large proportion of variance but may not measure exactly the same construct. This may be due to the differences in the underlying diagnostic systems or due to differences in momentary and retrospective assessments. Further validation through structured clinical interviews is indicated. The results suggest that ambulatory assessed mood indicators are a promising addition to multimodal depression screening tools. Improving app-based AA screenings requires adapted screening algorithms and corresponding methods for the analysis of dynamic processes over time.
Can open data increase younger generations’ trust in democratic institutions? A study in the European Union
Authors: Gonzálvez-Gallego Nicolás , Nieto-Torrejón Laura
Published: January 2021

Scholars and policy makers are giving increasing attention to how young people are involved in politics and their confidence in the current democratic system. In a context of a global trust crisis in the European Union, this paper examines if open government data, a promising governance strategy, may help to boost Millennials’ and Generation Z trust in public institutions and satisfaction with public outcomes. First, results from our preliminary analysis challenge some popular beliefs by revealing that younger generations tend to trust in their institutions notably more than the rest of the European citizens. In addition, our findings show that open government data is a trust-enabler for Millennials and Generation Z, not only through a direct link between both, but also thanks to the mediator role of citizens’ satisfaction. Accordingly, public officers are encouraged to spread the implementation of open data strategies as a way to improve younger generations’ attachment to democratic institutions.
Development of a reverse transcription recombinase polymerase amplification assay for rapid and direct visual detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
Authors: Lau Yee Ling , Ismail Ilyiana binti , Mustapa Nur Izati binti , Lai Meng Yee , Tuan Soh Tuan Suhaila , Haji Hassan Afifah , Peariasamy Kalaiarasu M. , Lee Yee Leng , Abdul Kahar Maria Kahar Bador , Chong Jennifer , Goh Pik Pin
Published: January 2021

Rapid diagnosis is an important intervention in managing the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) outbreak. Real time reverse transcription polymerase chain reaction (RT-qPCR) remains the primary means for diagnosing the new virus strain but it is time consuming and costly. Recombinase polymerase amplification (RPA) is an isothermal amplification assay that does not require a PCR machine. It is an affordable, rapid, and simple assay. In this study, we developed and optimized a sensitive reverse transcription (RT)-RPA assay for the rapid detection of SARS-CoV-2 using SYBR Green I and/or lateral flow (LF) strip. The analytical sensitivity and specificity of the RT-RPA assay were tested by using 10-fold serial diluted synthetic RNA and genomic RNA of similar viruses, respectively. Clinical sensitivity and specificity of the RT-RPA assay were carried out using 78 positive and 35 negative nasopharyngeal samples. The detection limit of both RPA and RT-qPCR assays was 7.659 and 5 copies/μL RNA, respectively with no cross reactivity with other viruses. The clinical sensitivity and specificity of RT-RPA were 98% and 100%, respectively. Our study showed that RT-RPA represents a viable alternative to RT-qPCR for the detection of SARS-CoV-2, especially in areas with limited infrastructure.