bluuebunny/crossref_metadata_2025 huggingface dataset:
Metabolome and transcriptome analyses provide new insights into the mechanisms underlying the enhancement of medicinal component content in the roots of Acanthopanax senticosus (Rupr. et Maxim.) Harms through foliar application of zinc fertilizer
Authors: Sun Tongze , Sun Jialin , Liu Yuli , Ren Yanjun , Li Yifeng , Shi Chang , Nasr Alyaa , Tang Zhonghua , Abozeid Ann
Published: November 2023

Acanthopanax senticosus (Rupr. et Maxim.) Harms is a perennial shrub of the Acanthopanax genus in the Araliaceae family and has a high medicinal value. The application of zinc fertilizer can improve the yield and quality of medicinal materials. However, there are limited reports on approaches to increase the content of medicinal components in A. senticosus, hindering the improvement of its medicinal quality. In this study, A. senticosus was treated with 0.1% (LZn) and 0.4% (HZn) zinc sprayed on the leaf surface. The effects of zinc treatment on the medicinal components in the roots of A. senticosus were analyzed by comprehensive metabolomics and transcriptomics analyses. A total of 316 metabolites were detected, with a prevailing occurrence of terpenoids and phenylpropanoids. We identified metabolites related to the medicinal components that were upregulated after Zn treatment, including 43 terpenoids, 19 phenylpropanoids, eight phenols, and three flavonoids. Combining differential gene expression and K-means analysis, we found 95, 65, and 25 upregulated genes related to phenylpropanoid biosynthesis, terpenoid biosynthesis, and flavonoid biosynthesis, respectively. Under different concentrations of Zn treatment, the upregulated metabolite biosynthesis-related genes and differentially expressed transcription factors varied. Pearson correlation network analysis revealed significant correlations among terpenoids, phenylpropanoids, flavonoids biosynthetic genes, and several transcription factors (ERFs, WRKYs, bHLHs, NACs, and MYBs). This study lays the foundation for understanding the metabolic processes in response to varying levels of zinc foliar spray and provides a theoretical basis for enhancing the efficiency of zinc fertilizer utilization in A. senticosus.
A third (booster) dose of the inactivated SARS-CoV-2 vaccine elicits immunogenicity and T follicular helper cell responses in people living with HIV
Authors: Lv Zhengchao , Lv Songqin , Li Qin , Xia Yafei , Feng Zaineng , Zhang Haohong , Yang Haihao , Wu Zhao , Zou Nanting , Mo Qingyan , Gu Qianlan , Ying Sai , Wang Xicheng , Qin Dongdong , Wan Chunping
Published: November 2023

Introduction

This study sought to explore the immunogenicity of a booster dose of an inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine in people living with human immunodeficiency virus (HIV) and identify the factors affecting the magnitude of anti-SARS-CoV-2 antibody levels.

Materials and methods

A total of 34 people living with HIV (PLWH) and 34 healthy donors (HD) were administered a booster dose of the same SARS-CoV-2 vaccine. Anti-SARS-CoV-2 antibody and immunoglobulin G (IgG) levels were measured using the SARS-CoV-2 S protein neutralizing antibody Enzyme-Linked Immunosorbent Assay (ELISA) and 2019-nCov IgG Chemiluminescent Immunoassay Microparticles, respectively. Spearman correlation analysis was used to measure the correlation between laboratory markers and neutralizing antibody and IgG levels. Peripheral blood mononuclear cells (PBMCs) were extracted from each subject using density gradient centrifugation and the numbers of memory T and T follicular helper (Tfh) cells were determined using flow cytometry.

Results

PLWH had a marked reduction in CD4 and B cell levels that was accompanied by a lower CD4/CD8 T cell ratio. However, those who received a supplementary dose of inactivated SARS-CoV-2 vaccines exhibited antibody positivity rates that were analogous to levels previously observed. The booster vaccine led to a reduction in IgG and neutralizing antibody levels and the amplitude of this decline was substantially higher in the PLWH than HD group. Correlation analyses revealed a strong correlation between neutralizing antibody levels and the count and proportion of CD4 cells. Anti-SARS-CoV-2 IgG antibody levels followed a similar trend. The expression of memory T and Tfh cells was considerably lower in the PLWH than in the HD group.

Discussion

PLWH had an attenuated immune response to a third (booster) administration of an inactivated SARS-CoV-2 vaccine, as shown by lower neutralizing antibody and IgG levels. This could be attributed to the reduced responsiveness of CD4 cells, particularly memory T and cTfh subsets. CD4 and cTfh cells may serve as pivotal markers of enduring and protective antibody levels. Vaccination dose recalibration may be critical for HIV-positive individuals, particularly those with a lower proportion of CD4 and Tfh cells.
Immuno-inflammatory in vitro hepatotoxicity models to assess side effects of biologicals exemplified by aldesleukin
Authors: Roser Luise A. , Luckhardt Sonja , Ziegler Nicole , Thomas Dominique , Wagner Pia Viktoria , Damm Georg , Scheffschick Andrea , Hewitt Philip , Parnham Michael J. , Schiffmann Susanne
Published: November 2023

Introduction

Hepatotoxicity induced by immunotherapeutics is an appearing cause for immune-mediated drug-induced liver injury. Such immuno-toxic mechanisms are difficult to assess using current preclinical models and the incidence is too low to detect in clinical trials. As hepatotoxicity is a frequent reason for post-authorisation drug withdrawal, there is an urgent need for immuno-inflammatory in vitro models to assess the hepatotoxic potential of immuno-modulatory drug candidates. We developed several immuno-inflammatory hepatotoxicity test systems based on recombinant human interleukin-2 (aldesleukin).

Methods

Co-culture models of primary human CD8+ T cells or NK cells with the hepatocyte cell line HepaRG were established and validated with primary human hepatocytes (PHHs). Subsequently, the HepaRG model was refined by increasing complexity by inclusion of monocyte-derived macrophages (MdMs). The main readouts were cytotoxicity, inflammatory mediator release, surface marker expression and specific hepatocyte functions.

Results

We identified CD8+ T cells as possible mediators of aldesleukin-mediated hepatotoxicity, with MdMs being implicated in increased aldesleukin-induced inflammatory effects. In co-cultures of CD8+ T cells with MdMs and HepaRG cells, cytotoxicity was induced at intermediate/high aldesleukin concentrations and perforin was upregulated. A pro-inflammatory milieu was created measured by interleukin-6 (IL-6), c-reactive protein (CRP), interferon gamma (IFN-γ), and monocyte chemoattractant protein-1 (MCP-1) increase. NK cells responded to aldesleukin, however, only minor aldesleukin-induced cytotoxic effects were measured in co-cultures. Results obtained with HepaRG cells and with PHHs were comparable, especially regarding cytotoxicity, but high inter-donor variations limited meaningfulness of the PHH model.

Discussion

The in vitro test systems developed contribute to the understanding of potential key mechanisms in aldesleukin-mediated hepatotoxicity. In addition, they may aid assessment of immune-mediated hepatotoxicity during the development of novel immunotherapeutics.
The neutrophil-lymphocyte ratio predicts all-cause and cardiovascular mortality among U.S. adults with rheumatoid arthritis: results from NHANES 1999-2020
Authors: Zhou Erye , Wu Jian , Zhou Xin , Yin Yufeng
Published: November 2023

Background

The neutrophil-to-lymphocyte ratio (NLR) is recognized as a biomarker for systemic inflammation and immune activation. However, its connection with the mortality risk in individuals with rheumatoid arthritis (RA) is not well understood. This study aimed to investigate the association between NLR and all-cause and cardiovascular mortality risk in U.S. adults with RA.

Methods

Data were gathered from the National Health and Nutrition Examination Survey (NHANES) cycles spanning 1999 to March 2020. We included adults aged ≥20 years. The NLR was computed by dividing the neutrophil count by the lymphocyte count from complete blood counts. The maximally selected rank statistics method helped identify the optimal NLR cutoff value associated with significant survival outcomes. Multivariable logistic regression models were performed to investigate the relationship between the NLR and the all-cause and cardiovascular mortality of RA. Restricted cubic spline (RCS) analyses were utilized to detect whether there were linear or non-linear relationships between NLR and mortality.

Results

In this study, 2002 adults with RA were included, with 339 having a higher NLR (≥3.28) and 1663 having a lower NLR (<3.28). During a median follow-up of 84 months, 79 RA individuals died. Participants with higher NLR had a 2-fold increased risk of all-cause (HR = 2.02, 95% CI: 1.53-2.66) and cardiovascular mortality (HR = 2.48, 95% CI: 1.34-4.57) versus lower NLR, after adjusting for demographics, socioeconomic status, and lifestyle factors. Kaplan-Meier analysis revealed that the survival rate for the higher NLR group was significantly lower than the lower NLR group, in terms of both all-cause and cardiovascular mortality (both P<0.0001). The RCS curve demonstrated a positive linear association between the NLR and all-cause and cardiovascular mortality.

Conclusion

A higher NLR was independently predictive of elevated long-term mortality risk in U.S. adults with RA. The NLR may serve as an inexpensive, widely available prognostic marker in RA.
Antimicrobial resistance genes and associated mobile genetic elements in Lactobacillales from various sources
Authors: Kaszab Eszter , Laczkó Levente , Kardos Gábor , Bányai Krisztián
Published: November 2023

Lactobacillales are commonly used in food products and as probiotics in animal and human medicine. Despite being generally recognized as safe, lactic acid bacteria may harbor a variety of antimicrobial resistance genes (ARGs), which may be transferable to human or veterinary pathogens, thus, may pose veterinary and public health concerns. This study investigates the resistome of Lactobacillales. A total of 4,286 whole-genome sequences were retrieved from NCBI RefSeq database. We screened ARGs in whole genome sequences and assessed if they are transmissible by plasmid transfer or by linkage to integrative mobile genetic elements. In the database, 335 strains were found to carry at least one ARG, and 194 strains carried at least one potentially transferable ARG. The most prevalent transferable ARG were tetM and tetW conferring antibiotic resistance to tetracycline. This study highlights the importance of the One Health concept by demonstrating the potential for Lactobacillales, commonly used in food products, to serve as reservoirs and vectors for ARGs.
Metabolic flux reprogramming in Mycobacterium tuberculosis-infected human macrophages
Authors: Borah Slater Khushboo , Moraes Luana , Xu Ye , Kim Daniel
Published: November 2023

Metabolic fluxes are at the heart of metabolism and growth in any living system. During tuberculosis (TB) infection, the pathogenic Mycobacterium tuberculosis (Mtb) adapts its nutritional behaviour and metabolic fluxes to survive in human macrophages and cause infection. The infected host cells also undergo metabolic changes. However, our knowledge of the infected host metabolism and identification of the reprogrammed metabolic flux nodes remains limited. In this study, we applied systems-based 13C-metabolic flux analysis (MFA) to measure intracellular carbon metabolic fluxes in Mtb-infected human THP-1 macrophages. We provide a flux map for infected macrophages that quantified significantly increased fluxes through glycolytic fluxes towards pyruvate synthesis and reduced pentose phosphate pathway fluxes when compared to uninfected macrophages. The tri carboxylic acid (TCA) cycle fluxes were relatively low, and amino acid fluxes were reprogrammed upon Mtb infection. The knowledge of host metabolic flux profiles derived from our work expands on how the host cell adapts its carbon metabolism in response to Mtb infection and highlights important nodes that may provide targets for developing new therapeutics to improve TB treatment.
Dynamic brain activity states of memory impairment in stroke patients with varying motor outcomes
Authors: Wang Caihong , Liu Jingchun , Guo Jun , Han Shaoqiang , Miao Peifang , Wei Ying , Wang Yingying , Wang Xin , Li Zhen , Xue Kangkang , Wang Kaiyu , Cheng Jingliang
Published: November 2023

Introduction

The objective of this study was to characterize the alteration patterns of dynamic spatiotemporal activity in chronic subcortical stroke patients with varying motor outcomes, while investigating the imaging indicators relevant to the assessment of potential cognitive deficits in these patients.

Methods

A total of 136 patients and 88 normal controls were included in the analysis of static and dynamic intrinsic brain activity, determined by amplitude of low-frequency fluctuations.

Results

The findings unveiled that subcortical stroke patients exhibited significantly aberrant temporal dynamics of intrinsic brain activity, involving regions within multiple brain networks. These spatiotemporal patterns were found to be contingent upon the side of the lesion. In addition, these aberrant metrics demonstrated potential in discerning cognitive deficits in stroke patients with memory impairment, with the dynamic indices exerting more influence than the static ones. The observe findings may indicate that subcortical stroke can trigger imbalances in the segregation and integration of spatiotemporal patterns across the entire brain with multi-domain networks, especially in patients with poor motor outcomes.

Conclusion

It suggests that the temporal dynamics indices of intrinsic brain activity could serve as potential imaging indicators for assessing cognitive impairment in patients with chronic subcortical stroke, which may be associated with the motor outcomes.
Ingestion of triglycerides containing medium- and long-chain fatty acids can increase metabolism of ingested long-chain triglycerides in overweight persons
Authors: Nosaka Naohisa , Tsujino Shogo , Sadamitsu Shohei , Ando Nanaka , Kato Kazuhiko
Published: November 2023

Introduction

Medium-chain fatty acids (MCFAs) have attracted considerable attention for preventing or improving obesity, which is a recognized risk factor for lifestyle-related diseases. Medium- and long-chain triglycerides (MLCTs) are expected to improve the metabolism of ingested long-chain triglycerides (LCTs). However, previous studies have reported mixed results. In this study, the effect of ingestion of MLCTs was evaluated on the metabolism of LCTs and compared to the ingestion of rapeseed oil (control oil).

Methods

A randomized, double-blind, placebo-controlled crossover study was performed among sedentary participants with BMIs ranging from 25 below 30 kg/m2. Thirty participants were asked to ingest either 14 g of MLCTs or a control oil for 4 weeks. The metabolism of ingested LCTs was evaluated by measuring isotopically labeled carbon dioxide released by the degradation of carbon-13 (13C)-labeled LCTs.

Results

Ingestion of MLCTs markedly enhanced the metabolism of ingested LCTs by comparison to the control oil.

Conclusion

The findings of this study suggest that ingestion of MLCTs may enhance the metabolism of dietary LCTs through activation of β-oxidation in liver mitochondria, which may increase the metabolic kinetics of ingested long-chain fatty acid (LCFAs).

Clinical trial registration

https://center6.umin.ac.jp/cgi-open-bin/ctr/ctr_view.cgi?recptno=R000053101, identifier: UMIN000046604.
Potential role of geranylgeraniol in managing statin-associated muscle symptoms: a COVID-19 related perspective
Authors: Tan Barrie , Chin Kok-Yong
Published: November 2023

Myopathy is the most common side effect of statins, but it has not been addressed effectively. In anticipation of its wider use as a small molecule to complement the current COVID-19 management, a pharmacological solution to statin-associated muscle symptoms (SAMS) is warranted. Statins act by suppressing the mevalonate pathway, which in turn affects the downstream synthesis of isoprenoids required for normal physiological functions. CoQ10 and geranylgeraniol (GG) syntheses are reduced by statin use. However, CoQ10 supplementation has not been shown to reverse SAMS. GG is an obligatory substrate for CoQ10 synthesis, an endogenous nutrient critical for skeletal muscle protein synthesis. Multiple studies showed GG supplementation is effective in reversing SAMS. This opinion paper proposes employing GG to prevent SAMS in pleiotropic statin use, including usage in the post-COVID-19 pandemic era.
The use of religious resources in helping victims of workplace mobbing
Authors: Vveinhardt Jolita , Deikus Mykolas
Published: November 2023

Research shows that in providing assistance to individuals who have experienced psychological traumas, it is beneficial not only to take into account the specific religious spiritual needs but also to employ religious resources. Although the role of religious counsellors using various psychological theories in helping to cope with traumatising experiences is acknowledged, there is still a lack of a conceptualising approach to the possibilities of employing religious resources used in Christian spiritual assistance, seeking to help cope with the effects of workplace mobbing. Therefore, this study aims to conceptualise the perspective of integrating Christian spiritual assistance resources in overcoming the individual consequences of workplace mobbing. This review is based on an interdisciplinary approach and abundant literature of psychology, psychotherapy and theology sciences. The article details the main physical, psychological and social aspects of damage to the person, caused by mobbing, which provide for a corresponding triple assistance perspective. After highlighting the essential resources provided by the Christian religion for coping with traumatic experiences, the necessity to consider the religious needs of the victims of mobbing is reasoned and the possibilities of using religious spiritual resources are discussed. In the context of helping victims of mobbing, two main functions of Christian spiritual assistance are distinguished and discussed: auxiliary and main. The results of this review are a useful resource for lay and religious counsellors and encourage their collaboration. The research findings also provide a basis for further research on the use of religious resources in the context of helping victims of mobbing.
Courtesy stigma among primary caregivers of children with autism spectrum disorder in eastern China
Authors: Chen Xu , Tong Jiao , Jiang Bochen , Ma Shan , Wang Xin , Sun Xun , Liu Yuan , Yan Dongmei , Wang Leilei
Published: November 2023

Introduction

The experience and perception of stigma is a common problem among primary caregivers of children with autism spectrum disorder (ASD), and has a profound adverse impact on primary caregivers and children with ASD; however, few studies have explored courtesy stigma among primary caregivers of children with ASD in the Chinese context. The aim of this study was to explore the status of courtesy stigma among primary caregivers of children with ASD in Lianyungang, Jiangsu Province, Eastern China, and to conduct in-depth analysis of its predictors from multiple perspectives.

Methods

An institution-based multi-center cross-sectional survey was conducted in the rehabilitation department of a large specialized hospital and 10 rehabilitation centers for children with special needs in Lianyungang, Jiangsu Province, Eastern China, from October 2022 to February 2023. A structured questionnaire to assess child-related factors, primary caregiver-related factors, courtesy stigma, general self-efficacy, and social support, was used to collect data. Predictors of courtesy stigma among primary caregivers of children with ASD were identified by linear regression.

Results

A total of 428 primary caregivers of children with ASD were recruited. The mean ± standard deviation (SD) score for courtesy stigma was 7.49 ± 4.13. Multiple linear regression analysis revealed that primary caregivers of children with ASD who were not too satisfied with their current marital status (β = 1.21, 95% CI: 0.34–2.08, p < 0.05) were more likely to have a high courtesy stigma; however, significantly lower courtesy stigma was observed in primary caregivers of children with ASD who were not picky eaters (β = −1.33, 95% CI: −2.08 – –0.58, p < 0.05), and who reported low level challenge in caring for children with ASD (β = −1.16, 95% CI: −2.20 – –0.12, p < 0.05), good general self-efficacy (β = −0.16, 95% CI: −0.25 – –0.06, p < 0.05), and good social support (β = −0.04, 95% CI: −0.08 – –0.01, p < 0.05).

Conclusion

There is a high level of courtesy stigma among primary caregivers of children with ASD in eastern China, and it is affected by numerous factors. More resources should be directed to groups that are more likely to experience stigma.
Impact of relationship status on psychological parameters in adults with congenital heart disease
Authors: Stapel Britta , Scharn Nicole , Halling Tim , Akkermann Steffen , Heitland Ivo , Westhoff-Bleck Mechthild , Kahl Kai G.
Published: November 2023

Objective

Adult congenital heart disease (ACHD) is a growing disease entity, posing questions concerning psychosocial outcomes across the lifespan. Spousal relationships were shown to benefit cardiovascular and mental health in the general population. We assessed the association of relationship status with anxiety and depression in ACHD patients and determined whether patients considered disease-related concerns potential mediators of relationship problems.

Methods

N = 390 ACHD patients were included. Self-report questionnaires were used to assess relationship status, ACHD-related relationship problems, socio-demographic variables, and depression and anxiety scores. Further, clinical parameters concerning the heart condition were determined.

Results

N = 278 (71%) patients were currently in a relationship, while N = 112 (29%) were not in a relationship. Groups did not significantly differ regarding age, sex, and cardiovascular parameters. Two-way MANCOVA with relationship status and sex as independent variables, controlling for age, NYHA class, and NT-proBNP, showed an association of relationship status with depression, while sex was associated with anxiety. N = 97 (25%) patients reported disease-related adverse effects on a current or prior relationship. In detail, worries about body image (N = 57, 61%), own fears (N = 51, 54%), problems arising from wish to have children (N = 33, 35%), fears regarding a joint future (N = 29, 31%), partner’s fears or lack of understanding (N = 28, 30%), and sexual problems (N = 21, 22%) were cited.

Conclusion

Relationships status was associated with depression, while sex was associated with anxiety in ACHD patients. Relationship status as well as potential relationship problems, and the importance of social support for mental and physical well-being, should be considered when treating ACHD patients.
Problems of knowledge, problems of order: the open science field site
Authors: Goldensher Liora O’Donnell
Published: November 2023

Ethnographers can and should not justdoornot doopen science, butstudythe push to share data, instruments, and other research materials as an important moment of change and contest in contemporary knowledge-making and knowledge politics. Following ethnographers of science and technology who have demonstrated the analytic opportunities afforded by moments of scientific controversy, we should treat the places where these calls are made, debated, and taken up as important field sites for ethnographic inquiry. Whenever and wherever the sharing of data, instruments, and research is discussed, planned, done, measured, judged, or regulated, there are powerful claims, visions, and action concerning what makes for facticity, legitimacy, and credibility in both research and politics. From these sites, I argue, we can observe changes to disciplinary and popular understandings of epistemic virtue, or what makes for reliable, factual, or adequately transparent knowledge production. Attention to these sites can also yield important perspectives on the ways that visions of proper research conduct are imbricated with visions of governance. I argue that turning ethnographic methods to studying the open science movement can enable us to do timely scholarship about shifting understandings of facticity, knowledge, information, and governance.
Effects of hip osteoarthritis on lower body joint kinematics during locomotion tasks: a systematic review and meta-analysis
Authors: Steingrebe Hannah , Spancken Sina , Sell Stefan , Stein Thorsten
Published: November 2023

Introduction

Motion analysis can be used to gain information needed for disease diagnosis as well as for the design and evaluation of intervention strategies in patients with hip osteoarthritis (HOA). Thereby, joint kinematics might be of great interest due to their discriminative capacity and accessibility, especially with regard to the growing usage of wearable sensors for motion analysis. So far, no comprehensive literature review on lower limb joint kinematics of patients with HOA exists. Thus, the aim of this systematic review and meta-analysis was to synthesise existing literature on lower body joint kinematics of persons with HOA compared to those of healthy controls during locomotion tasks.

Methods

Three databases were searched for studies on pelvis, hip, knee and ankle kinematics in subjects with HOA compared to healthy controls during locomotion tasks. Standardised mean differences were calculated and pooled using a random-effects model. Where possible, subgroup analyses were conducted. Risk of bias was assessed with the Downs and Black checklist.

Results and Discussion

A total of 47 reports from 35 individual studies were included in this review. Most studies analysed walking and only a few studies analysed stair walking or turning while walking. Most group differences were found in ipsi- and contralateral three-dimensional hip and sagittal knee angles with reduced ranges of motion in HOA subjects. Differences between subjects with mild to moderate and severe HOA were found, with larger effects in severe HOA subjects. Additionally, stair walking and turning while walking might be promising extensions in clinical gait analysis due to their elevated requirements for joint mobility. Large between-study heterogeneity was observed, and future studies have to clarify the effects of OA severity, laterality, age, gender, study design and movement execution on lower limb joint kinematics.

Systematic Review Registration

PROSPERO (CRD42021238237).
Enzymolytic soybean meal improves growth performance, economic efficiency and organ development associated with cecal fermentation and microbiota in broilers offered low crude protein diets
Authors: Zhu Xin , Gao Kai , Qi Yongqiang , Yang Guiqin , Liu Haiying
Published: November 2023

The objective of this experiment was to determine the effect of low crude protein (CP) diets containing increasing amounts of enzymolytic soybean meal (ESBM) on growth performance, economic benefit and organ development and the role of cecal fermentation and microbiota in broilers. A total of 360 one-day-old Arbor Acres chicks were randomly allocated into 6 groups with 6 replicates and 10 chicks each. The six dietary treatments consisted of a standard high-CP diet (PC), a low-CP diet (NC), and an NC diet with 0.5, 1.0, 1.5%, or 2.0% ESBM. The experiment lasted for 42 days. Compared to PC, NC showed decreased (p < 0.05) average daily gain (ADG) in broilers from 22 to 42 days and from 1 to 42 days, while increasing levels of ESBM quadratically increased (p < 0.05) ADG from 1 to 42 days. Feed cost and total revenue in the NC were lower (p < 0.05) than that in the PC, while supplementation with ESBM in the NC linearly increased (p < 0.05) net profit and economic efficiency in broilers. There were significant differences (p < 0.05) in the liver, proventriculus and gizzard indices between the PC and NC groups, and supplementation with ESBM linearly increased (p < 0.05) the relative weights of liver, pancreas, proventriculus and gizzard in broilers at 42 days of age. The PC group had a higher cecal acetic acid concentration at 21 days and propionic acid concentration at both 21 and 42 days than the NC group (p < 0.05). Cecal acetic acid and propionic acid concentrations linearly increased (p < 0.05) with increasing levels of ESBM in broilers at 42 days of age. No significant differences in ACE, Chao1, Shannon and Simpson indices were observed among groups (p > 0.05), while the cecal abundances of Bacteroides, Faecalibacterium and Clostridium IV increased (p < 0.05) with the increasing level of ESBM in the low-CP diets. In conclusion, feeding ESBM improved economic efficiency, digestive organ development, cecal fermentation and microbial community composition, and up to 2.0% ESBM addition had no negative effect on the growth performance in broilers fed low CP diets.
Fabrication of a Gd2O3-Based Film to Shield from Space Radiation inside Aircraft and Its Effectiveness
Authors: Kim Seon-Chil , Kim Sung-Hwan
Published: November 2023

Aircraft are exposed to cosmic radiation depending on their flight altitude and latitude. Therefore, flight attendants are exposed to radiation for long periods. In this study, a 0.3 mm thick fabric was designed with which to manufacture crew clothes to shield them against external exposure to space radiation, and the shielding performance was analyzed based on empirical experiments in a real environment. Gadolinium oxide, which has a high neutron reaction cross-section, and tungsten, which is useful for gamma-ray shielding, were proposed as the main raw materials for the shielding fabric, and the shielding performance was evaluated using detectors on Arctic flight routes. Composite (KG-01) and single (KG-02) shielding materials were used. In the case of KG-01, the transmission dose rate was 90.7 ± 5.6% compared with the unshielded case, showing an average space-radiation dose reduction of 9.3%. With KG-02, the transmission dose rate was 103.1 ± 2.0% compared with the unshielded case, and the average dose rate increased by 3.1%; therefore, there was no shielding effect against space radiation. Considering the statistical error of the environmental radiation at aircraft flight altitudes, KG-01 had a shielding effect of at least 5%; however, KG-02 yielded no significant shielding effects.
Element Composition of Fractionated Water-Extractable Soil Colloidal Particles Separated by Track-Etched Membranes
Authors: Volkov Dmitry S. , Rogova Olga B. , Ovseenko Svetlana T. , Odelskii Aleksandr , Proskurnin Mikhail A.
Published: November 2023

Membrane fractionation with track-etched membranes was used to size-profile the microelement composition of water-extractable soil colloids (WESCs). The aim of the study is the element composition of narrow WESC fractions of typical chernozems in the range of 0.01–10 µm. Micro-/ultrafiltration through a cascade of track-etched polycarbonate membrane filters with pore sizes of 5, 2, 1, 0.8, 0.4, 0.2, 0.1, 0.05, 0.03, and 0.01 µm at room temperature was used. ICP–AES using direct spraying of obtained fractions without decomposition was used; Al, Ba, Cd, Cr, Cu, Fe, Mn, Si, Sr, Ti, Zn, Ca, K, Mg, Na, P, and S were found. Narrow WESC fractions differ significantly. For macro- and microelements, maximum amounts of Si, Al, Fe, and Ti and their maximum percentages are observed in fractions with sizes above 1 µm, while Ca, Mg, Mn, Cu, Zn, K, and S are accumulated more in fractions with sizes below 1 µm. The developed approach provides preparative isolation of a detailed set of narrow WESC fractions in the micrometer–nanometer range. This provides element soil profiles that reveal distinct differences and the individual character of each fraction as well as trends in changes in the mineral matrix and microelement composition with fraction size.
Field Phenotyping Monitoring Systems for High-Throughput: A Survey of Enabling Technologies, Equipment, and Research Challenges
Authors: Yuan Huali , Song Minghan , Liu Yiming , Xie Qi , Cao Weixing , Zhu Yan , Ni Jun
Published: November 2023

High-throughput phenotype monitoring systems for field crops can not only accelerate the breeding process but also provide important data support for precision agricultural monitoring. Traditional phenotype monitoring methods for field crops relying on artificial sampling and measurement have some disadvantages including low efficiency, strong subjectivity, and single characteristics. To solve these problems, the rapid monitoring, acquisition, and analysis of phenotyping information of field crops have become the focus of current research. The research explores the systematic framing of phenotype monitoring systems for field crops. Focusing on four aspects, namely phenotyping sensors, mobile platforms, control systems, and phenotyping data preprocessing algorithms, the application of the sensor technology, structural design technology of mobile carriers, intelligent control technology, and data processing algorithms to phenotype monitoring systems was assessed. The research status of multi-scale phenotype monitoring products was summarized, and the merits and demerits of various phenotype monitoring systems for field crops in application were discussed. In the meantime, development trends related to phenotype monitoring systems for field crops in aspects including sensor integration, platform optimization, standard unification, and algorithm improvement were proposed.
Heat Inactivation of Methicillin-Resistant Staphylococcus aureus Strains from German Dairy farms in Colostrum and Raw Milk
Authors: Wörmann Mirka E. , Bhatte Ashwini , Wichmann-Schauer Heidi , Tenhagen Bernd-Alois , Lienen Tobias
Published: November 2023

Methicillin-resistant Staphylococcus aureus (MRSA) may cause difficult-to-treat infections in dairy cattle. One possible route of MRSA transmission into calves is via the feeding of contaminated waste milk. We tested the heat resistance of 17 MRSA strains isolated from German dairy farms in colostrum and raw milk in a laboratory approach. Heating colostrum or raw milk at 60 °C for 30 min eliminated all viable MRSA in the milk, provided the MRSA inoculation rate is low (103 cfu mL−1). In contrast, raw milk highly inoculated with MRSA (106 cfu mL−1) required a holding time of at least 30 min at 70 °C to fully eliminate MRSA from it. However, quantitative analysis showed that a heat treatment for 10 min at 60 °C already significantly reduced the number of viable MRSA in highly inoculated raw milk. Heating colostrum and raw milk above 60 °C may destroy immunoglobulins which are crucial for the calf’s health. Therefore, we suggest that colostrum and raw milk that is to be fed to calves on MRSA-positive dairy farms is heated at 60 °C for at least 10 min to reduce the likelihood of transmitting MRSA. In addition, the 60 °C heat-treated colostrum/raw milk should be fed to the calves as soon as possible to avoid re-growth of viable MRSA.
Hepatoprotective Mechanisms Induced by Spinach Methanolic Extract in Rats with Hyperglycemia—An Immunohistochemical Analysis
Authors: Flores-Estrada Javier , Cano-Martínez Agustina , Vargas-González Álvaro , Castrejón-Téllez Vicente , Cornejo-Garrido Jorge , Martínez-Rosas Martín , Guarner-Lans Verónica , Rubio-Ruíz María Esther
Published: November 2023

Spinach methanolic extract (SME) has a hepatoprotective effect due to its polyphenolic antioxidants; however, its action in parenchymal (PQ) and non-parenchymal (nPQ) cells remains unknown. This study investigates the hepatoprotective effect of SME on streptozotocin-induced hyperglycemic rats (STZ), focusing on immunohistochemical analyses. Methods: The extract was prepared, and the total polyphenols and antioxidant activity were quantified. Adult male Wistar rats were divided into four groups (n = 8): normoglycemic rats (NG), STZ-induced hyperglycemic (STZ), STZ treated with 400 mg/kg SME (STZ-SME), and NG treated with SME (SME) for 12 weeks. Serum liver transaminases and lipid peroxidation levels in tissue were determined. The distribution pattern and relative levels of markers related to oxidative stress [reactive oxygen species (ROS), superoxide dismutase-1, catalase, and glutathione peroxidase-1], of cytoprotective molecules [nuclear NRF2 and heme oxygenase-1 (HO-1)], of inflammatory mediators [nuclear NF-κB, TNF-α], proliferation (PCNA), and of fibrogenesis markers [TGF-β, Smad2/3, MMP-9, and TIMP1] were evaluated. Results: SME had antioxidant capacity, and it lowered serum transaminase levels in STZ-SME compared to STZ. It reduced NOX4 staining, and lipid peroxidation levels were related to low formation of ROS. In STZ-SME, the immunostaining for antioxidant enzymes increased in nPQ cells compared to STZ. However, enzymes were also localized in extra and intracellular vesicles in STZ. Nuclear NRF2 staining and HO-1 expression in PQ and nPQ were higher in STZ-SME than in STZ. Inflammatory factors were decreased in STZ-SME and were related to the percentage decrease in NF-κB nuclear staining in nPQ cells. Similarly, TGF-β (in the sinusoids) and MMP-9 (in nPQ) were increased in the STZ-SME group compared to the other groups; however, staining for CTGF, TIMP1, and Smad2/3 was lower. Conclusions: SME treatment in hyperglycemic rats induced by STZ may have hepatoprotective properties due to its scavenger capacity and the regulation of differential expression of antioxidant enzymes between the PQ and nPQ cells, reducing inflammatory and fibrogenic biomarkers in liver tissue.
A Hybrid Method for Technical Condition Prediction Based on AI as an Element for Reducing Supply Chain Disruptions
Authors: Kuźnar Małgorzata , Lorenc Augustyn
Published: November 2023

In the field of transport, and more precisely in supply chains, if any of the vehicle components are damaged, it may cause delays in the delivery of goods. Eliminating undesirable damage to the means of transport through the possibility of predicting technical conditions and a state of failure may increase the reliability of the entire supply chain. From the aspect of sustainability, the issue of reducing the number of failures also makes it possible to reduce supply chain disturbances, to reduce costs associated with delays, and to reduce the materials needed for the repair of the means of transport, since, in this case, the costs only relate to the replaced elements before their damage. Thus, it is impossible for more serious damage to occur. Often, failure of one item causes damage to others, which generates unnecessary costs and increases the amount of waste due to the number of damaged items. This article provides an author’s method of technical condition prediction; by applying the method, it would be possible to develop recommended maintenance activities for key elements related to the safety and reliability of transport. The combination of at least two artificial intelligence methods allows us to achieve very good prediction results thanks to the possibility of individual adjustments of weights between the methods used. Such predictive maintenance methods can be successfully used to ensure sustainable development in supply chains.
Zn Doping Improves the Anticancer Efficacy of SnO2 Nanoparticles
Authors: Alanazi Sitah , Alaizeri ZabnAllah M. , Lateef Rashid , Madkhali Nawal , Alharbi Abdullah , Ahamed Maqusood
Published: November 2023

Tin dioxide (SnO2) nanoparticles (NPs) can be applied in several ways due to their low cost, high surface-to-volume ratio, facile synthesis, and chemical stability. There is limited research on the biomedical application of SnO2-based nanostructures. This study aimed to investigate the role of Zn doping in relation to the anticancer potential of SnO2 NPs and to enhance the anticancer potential of SnO2 NPs through Z doping. Pure SnO2 and Zn-doped SnO2 NPs (1% and 5%) were prepared using a modified sol–gel route. XRD, TEM, SEM, EDX, UV-Vis, FTIR, and PL techniques were used to characterize the physicochemical properties of produced NPs. XRD analysis revealed that the crystalline size and phase composition of pure SnO2 increased after the addition of Zn. The spherical shape and homogenous distribution of these NPs were confirmed using TEM and SEM techniques. EDX analysis confirmed the Sn, Zn, and O elements in Zn-SnO2 NPs without impurities. Zn doping decreased the band gap energy of SnO2 NPs. The PL study indicated a reduction in the recombination rate of charges (electrons/holes) in SnO2 NPs after Zn doping. In vitro studies showed that the anticancer efficacy of SnO2 NPs increased with increasing levels of Zn doping in breast cancer MCF-7 cells. Moreover, pure and Zn-doped SnO2 NPs showed good cytocompatibility in HUVECs. This study emphasizes the need for additional investigation into the anticancer properties of Zn-SnO2 nanoparticles in various cancer cell lines and appropriate animal models.
Risk Assessment in the Design of Railroad Control Command and Signaling Devices Using Fuzzy Sets
Authors: Ilczuk Przemysław , Kycko Magdalena
Published: November 2023

Risk assessment in the design of control command and signaling devices (CCS) is one of the elements required by law. These analyses should be carried out at many stages of investment with the participation of various teams. This article presents a risk analysis method based on fuzzy sets, which can support and increase the safety of investment processes involving the railroad traffic control industry. The article analyzes hazards identified in CCS design. These risks were identified using a survey method based on a set of questions prepared by the authors and by conducting interviews among experts from design offices. As part of the survey, responses were obtained from 28 respondents who are specialists in the railway traffic control industry. Workshop meetings were held in six different design offices and at manufacturing plants of motion control systems. The identified risks were analyzed using the FMEA (failure mode and effect analysis) method and the fuzzy set method, as well as various methods of fuzzification and defuzzification. The results of all of the methods were compared with each other. The best solution from the analyzed ones was proposed.
Development and Investigation of the Hysteretic Behavior of an X-Shaped Metal Damper with an Oblique Angle
Authors: Zhu Xiaojun , Dang Longji , Liang Shuting , Zhang Ming , Yang Jian , Dai Xin
Published: November 2023

To investigate the hysteretic behavior of an X-shaped metal damper (XMD) with an oblique angle, cyclic loading tests were carried out on nine specimens, including two XMDs without buckling-restrained devices, four XMDs with stiffening ribs, and three XMDs with cover plates as references. The test results showed that the oblique angle could effectively increase the stiffness, strength, and energy dissipation of the XMD. When the oblique angle of an XMD with stiffening ribs increased from 0° to 30° at the applied displacement of 8.4 mm, the mean strengths and cumulative energy dissipation of specimens increased by about 80.77% and 80.57%, respectively. Although asymmetric hysteretic loops were also observed in specimens with an oblique angle and buckling-restrained devices, stable hysteretic curves were obtained. This indicated that the stiffening ribs and cover plates can effectively constrain the buckling behavior of XMDs. Additionally, the mean strengths of specimens with stiffening ribs were a little higher than those of specimens with cover plates. Subsequently, the finite element analysis models of the XMDs were proposed, in which the metal plasticity model considering isotropic and kinematical hardening was used to model the material properties of steel, and the simulation results matched well with the test results. Finally, the theoretical calculation method was proposed to predict the elastic stiffness of specimens, and the theoretical elastic stiffness matched well with the test results.
The Elusive Nature of “Seeing”
Authors: Tokovinin Andrei
Published: November 2023

Atmospheric image blur, “seeing”, is one of the key parameters that influences the selection of observatory sites and the performance of ground-based telescopes. In this review, the common definition of seeing based on the Kolmogorov turbulence model is recalled. The ability of this model to represent real, non-stationary fluctuations of the air refractive index is discussed. Even in principle, seeing (a model parameter) cannot be measured with arbitrary accuracy; consequently, describing atmospheric blur by a single number, seeing, is a crude approximation. The operating principles of current seeing monitors are outlined. They measure optical effects caused by turbulence, sampling certain regions of spatial and temporal spectrum of atmosphreic optical disturbances, and interpret their statistics in the framework of the standard model. Biases of seeing monitors (measurement noise, propagation, finite exposure time, optical defects, wind shake, etc.) should be quantified and corrected using simulations, while instrument comparison campaigns serve as a check. The elusive nature of seeing follows from its uniqueness (a given measurement cannot be repeated or checked later), its non-stationarity (dependence on time, location, and viewing direction), a substantial role of the highly variable surface layer, and a potential bias caused by the air flow in the immediate vicinity of the seeing monitors. The results of seeing measurements are outside the scope of this review.
Contrasting the Impacts of Intraseasonal Oscillations on Yangtze Precipitation during the Summer of 1998 and 2016
Authors: Tao Mimi , Yan Li , Zheng Shaojun , Xu Jianjun , Chen Yinlan
Published: November 2023

In 1998 and 2016, boreal summer intraseasonal oscillation (BSISO) could reach the middle-lower reaches of the Yangtze River basin (YRB), leading to extreme precipitation. Based on multiple daily data, this study reveals the differences in BSISO events and mechanisms between 1998 and 2016. In June–July of 1998 (2016), YRB precipitation was impacted by 30–60-day oscillation, i.e., BSISO1 (10–30-day oscillation, i.e., BSISO2), with two strong (three) precipitation events occurring. In 1998, when BSISO1 was in phases 1–4 (phases 5–8), the YRB experienced a wet (dry) episode. In 2016, when BSISO2 was in phases 1–2 and 7–8 (phases 3–6), the YRB experienced a wet (dry) episode. In 1998, in event 1, the active convection of the YRB first originated in the South China Sea–western Pacific (SCS–WP) and then in the tropical Indian Ocean (IO). In 1998, in event 2, the active convection of the YRB originated in the SCS–WP. In 2016, in events 1 and 3, the active convection of the YRB originated from the SCS–WP. In 2016, in event 2, the active convection of the YRB originated from the tropical IO and the extratropical WP. Different SST and atmospheric circulations explain different BSISO modes that dominate in the YRB. In 1998 (2016), in summer, (no) strong easterly wind anomalies occurred in the SCS–WP, which are favorable (unfavorable) for the enhancement of BSISO1. Accompanying the suppressed BSISO1, BSISO2 was enhanced in 2016.