bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigation of the Effect of Therapeutic Plasma Exchange for TAFRO Syndrome: A Pilot Study
Authors: Sonoda Kosuke , Komatsu Masamichi , Ozawa Yoko , Yamamoto Hiroshi , Kamijo Yuji
Published: April 2024

TAFRO syndrome is a rare systemic inflammatory disorder with a fatal course. Nevertheless, a definitive treatment strategy has not yet been established. Anti-inflammatory therapies, including glucocorticoid treatment and immunosuppressants, have not been satisfactory. Therefore, new treatment options are needed for patients with TAFRO syndrome. The effectiveness of therapeutic plasma exchange (TPE) has mainly been reported in several case reports. In this case series study, we investigated the effect of TPE on TAFRO syndrome. We reviewed six consecutive cases with TAFRO syndrome treated at Shinshu University Hospital. All of them underwent TPE. A significant improvement in mean blood pressure, albumin, total bilirubin, and C-reactive protein was observed after TPE. Furthermore, early TPE treatment was suggested to have an impact on the prognosis. More intensive studies are needed to emphasize the overall conclusion obtained that TPE can be an effective/acceptable treatment option for TAFRO syndrome.
Aspartame Causes Developmental Defects and Teratogenicity in Zebra Fish Embryo: Role of Impaired SIRT1/FOXO3a Axis in Neuron Cells
Authors: Pandaram Athiram , Paul Jeyakumari , Wankhar Wankupar , Thakur Abhimanyu , Verma Sakshi , Vasudevan Karthick , Wankhar Dapkupar , Kammala Ananth Kumar , Sharma Priyanshu , Jaganathan Ravindran , Iyaswamy Ashok , Rajan Ravindran
Published: April 2024

Aspartame, a widely used artificial sweetener, is present in many food products and beverages worldwide. It has been linked to potential neurotoxicity and developmental defects. However, its teratogenic effect on embryonic development and the underlying potential mechanisms need to be elucidated. We investigated the concentration- and time-dependent effects of aspartame on zebrafish development and teratogenicity. We focused on the role of sirtuin 1 (SIRT1) and Forkhead-box transcription factor (FOXO), two proteins that play key roles in neurodevelopment. It was found that aspartame exposure reduced the formation of larvae and the development of cartilage in zebrafish. It also delayed post-fertilization development by altering the head length and locomotor behavior of zebrafish. RNA-sequencing-based DEG analysis showed that SIRT1 and FOXO3a are involved in neurodevelopment. In silico and in vitro analyses showed that aspartame could target and reduce the expression of SIRT1 and FOXO3a proteins in neuron cells. Additionally, aspartame triggered the reduction of autophagy flux by inhibiting the nuclear translocation of SIRT1 in neuronal cells. The findings suggest that aspartame can cause developmental defects and teratogenicity in zebrafish embryos and reduce autophagy by impairing the SIRT1/FOXO3a axis in neuron cells.
Inhibition of Metalloproteinases Extends Longevity and Function of In Vitro Human iPSC-Derived Skeletal Muscle
Authors: Barakat Natali , Jangir Himanshi , Gallo Leandro , Grillo Marcella , Guo Xiufang , Hickman James
Published: April 2024

In vitro culture longevity has long been a concern for disease modeling and drug testing when using contractable cells. The dynamic nature of certain cells, such as skeletal muscle, contributes to cell surface release, which limits the system’s ability to conduct long-term studies. This study hypothesized that regulating the extracellular matrix (ECM) dynamics should be able to prolong cell attachment on a culture surface. Human induced pluripotent stem cell (iPSC)-derived skeletal muscle (SKM) culture was utilized to test this hypothesis due to its forceful contractions in mature muscle culture, which can cause cell detachment. By specifically inhibiting matrix metalloproteinases (MMPs) that work to digest components of the ECM, it was shown that the SKM culture remained adhered for longer periods of time, up to 80 days. Functional testing of myofibers indicated that cells treated with the MMP inhibitors, tempol, and doxycycline, displayed a significantly reduced fatigue index, although the fidelity was not affected, while those treated with the MMP inducer, PMA, indicated a premature detachment and increased fatigue index. The MMP-modulating activity by the inhibitors and inducer was further validated by gel zymography analysis, where the MMP inhibitor showed minimally active MMPs, while the inducer-treated cells indicated high MMP activity. These data support the hypotheses that regulating the ECM dynamics can help maximize in vitro myotube longevity. This proof-of-principle strategy would benefit the modeling of diseases that require a long time to develop and the evaluation of chronic effects of potential therapeutics.
RNA-Binding Protein Motifs Predict microRNA Secretion and Cellular Retention in Hypothalamic and Other Cell Types
Authors: He Wenyuan , Belsham Denise D.
Published: April 2024

Cellular microRNAs (miRNAs) can be selectively secreted or retained, adding another layer to their critical role in regulating human health and disease. To date, select RNA-binding proteins (RBPs) have been proposed to be a mechanism underlying miRNA localization, but the overall relevance of RBPs in systematic miRNA sorting remains unclear. This study profiles intracellular and small extracellular vesicles’ (sEVs) miRNAs in NPY-expressing hypothalamic neurons. These findings were corroborated by the publicly available sEV and intracellular miRNA profiles of white and brown adipocytes, endothelium, liver, and muscle from various databases. Using experimentally determined binding motifs of 93 RBPs, our enrichment analysis revealed that sEV-originating miRNAs contained significantly different RBP motifs than those of intracellularly retained miRNAs. Multiple RBP motifs were shared across cell types; for instance, RBM4 and SAMD4 are significantly enriched in neurons, hepatocytes, skeletal muscle, and endothelial cells. Homologs of both proteins physically interact with Argonaute1/2 proteins, suggesting that they play a role in miRNA sorting. Machine learning modelling also demonstrates that significantly enriched RBP motifs could predict cell-specific preferential miRNA sorting. Non-optimized machine learning modeling of the motifs using Random Forest and Naive Bayes in all cell types except WAT achieved an area under the receiver operating characteristic (ROC) curve of 0.77–0.84, indicating a high predictive accuracy. Given that the RBP motifs have a significant predictive power, these results underscore the critical role that RBPs play in miRNA sorting within mammalian cells and reinforce the importance of miRNA sequencing in preferential localization. For the future development of small RNA therapeutics, considering these RBP-RNA interactions could be crucial to maximize delivery effectiveness and minimize off-target effects.
Significant Genes Associated with Mortality and Disease Progression in Grade II and III Glioma
Authors: Choi Bo Mi , Cheong Jin Hwan , Ryu Je Il , Won Yu Deok , Min Kyueng-Whan , Han Myung-Hoon
Published: April 2024

Background: The Wnt/β-catenin pathway plays a critical role in the tumorigenesis and maintenance of glioma stem cells. This study aimed to evaluate significant genes associated with the Wnt/β-catenin pathway involved in mortality and disease progression in patients with grade II and III glioma, using the Cancer Genome Atlas (TCGA) database. Methods: We obtained clinicopathological information and mRNA expression data from 515 patients with grade II and III gliomas from the TCGA database. We performed a multivariate Cox regression analysis to identify genes independently associated with glioma prognosis. Results: The analysis of 34 genes involved in Wnt/β-catenin signaling demonstrated that four genes (CER1, FRAT1, FSTL1, and RPSA) related to the Wnt/β-catenin pathway were significantly associated with mortality and disease progression in patients with grade II and III glioma. We also identified additional genes related to the four significant genes of the Wnt/β-catenin pathway mentioned above. The higher expression of BMP2, RPL18A, RPL19, and RPS12 is associated with better outcomes in patients with glioma. Conclusions: Using a large-scale open database, we identified significant genes related to the Wnt/β-catenin signaling pathway associated with mortality and disease progression in patients with grade II and III gliomas.
Understanding Tendon Fibroblast Biology and Heterogeneity
Authors: DiIorio Sarah E. , Young Bill , Parker Jennifer B. , Griffin Michelle F. , Longaker Michael T.
Published: April 2024

Tendon regeneration has emerged as an area of interest due to the challenging healing process of avascular tendon tissue. During tendon healing after injury, the formation of a fibrous scar can limit tendon strength and lead to subsequent complications. The specific biological mechanisms that cause fibrosis across different cellular subtypes within the tendon and across different tendons in the body continue to remain unknown. Herein, we review the current understanding of tendon healing, fibrosis mechanisms, and future directions for treatments. We summarize recent research on the role of fibroblasts throughout tendon healing and describe the functional and cellular heterogeneity of fibroblasts and tendons. The review notes gaps in tendon fibrosis research, with a focus on characterizing distinct fibroblast subpopulations in the tendon. We highlight new techniques in the field that can be used to enhance our understanding of complex tendon pathologies such as fibrosis. Finally, we explore bioengineering tools for tendon regeneration and discuss future areas for innovation. Exploring the heterogeneity of tendon fibroblasts on the cellular level can inform therapeutic strategies for addressing tendon fibrosis and ultimately reduce its clinical burden.
The Role of Bioceramics for Bone Regeneration: History, Mechanisms, and Future Perspectives
Authors: Tanvir Md Amit Hasan , Khaleque Md Abdul , Kim Ga-Hyun , Yoo Whang-Yong , Kim Young-Yul
Published: April 2024

Osteoporosis is a skeletal disorder marked by compromised bone integrity, predisposing individuals, particularly older adults and postmenopausal women, to fractures. The advent of bioceramics for bone regeneration has opened up auspicious pathways for addressing osteoporosis. Research indicates that bioceramics can help bones grow back by activating bone morphogenetic protein (BMP), mitogen-activated protein kinase (MAPK), and wingless/integrated (Wnt)/β-catenin pathways in the body when combined with stem cells, drugs, and other supports. Still, bioceramics have some problems, such as not being flexible enough and prone to breaking, as well as difficulties in growing stem cells and discovering suitable supports for different bone types. While there have been improvements in making bioceramics better for healing bones, it is important to keep looking for new ideas from different areas of medicine to make them even better. By conducting a thorough scrutiny of the pivotal role bioceramics play in facilitating bone regeneration, this review aspires to propel forward the rapidly burgeoning domain of scientific exploration. In the end, this appreciation will contribute to the development of novel bioceramics that enhance bone regrowth and offer patients with bone disorders alternative treatments.
Manipulations in Democracy?
Authors: Ben-Yashar Ruth
Published: April 2024

Democracy is upheld through the principle of majority rule. To validate the application of democracy, it is imperative to assess the sincerity of voter decisions. When voter sincerity is compromised, manipulation may occur, thereby undermining the legitimacy of democratic processes. This paper presents a general version of a symmetric dichotomous choice model. Using simple majority rule, we show that when a voter receives one or more private signals, sincere voting is an equilibrium behavior. A slight change to this basic model may create an incentive to vote insincerely. We show that even in a more restricted model where every voter receives only one private signal whose level of precision is the same for all the voters but depends on the state of nature, voters may have an incentive to vote insincerely.
Red Code Management in a Pediatric Emergency Department: A Retrospective Study
Authors: Bertone Serena , Denina Marco , Pagano Manuela , Delmonaco Angelo Giovanni , Castagno Emanuele , Bondone Claudia
Published: April 2024

The “red code” (RC) represents the highest level of emergency in the emergency department (ED). This study retrospectively analyzed RCs in the Regina Margherita Children’s Hospital ED, a regional referral center in north Italy, between 1 July 2020 and 30 June 2023. The aim was to describe RC characteristics and to identify significant correlations between presenting complaints and clinical management. The study includes 934 RCs (0.9% of overall ED admissions); 64% were assigned based on the Pediatric Assessment Triangle alteration. Most patients, 86.5%, followed the medical pathway, while 13.5% were surgical cases. Admission complaints were respiratory (46.9%), neuropsychiatric (26.7%), traumatic (11.8%), cardiologic (9.3%), metabolic (3.8%), and surgical (1.5%). Seventy-six percent of patients received vascular access, and intraosseous access was obtained in 2.2% of them. In one-third of RCs, an urgent critical care evaluation was necessary, and 19% of cases required admission to the intensive care unit. The overall mortality rate was 3.4% (0.4% in ED setting). The study identified six distinct diagnostic pathways, each associated with specific characteristics in clinical presentation, management, therapeutic interventions, and outcomes. Our findings underscore the need for a systematic approach in pediatric emergency settings, supported by international and national guidelines but also by clearly defined diagnostic pathways, aiming to enhance the quality of care and patient outcomes.
Calculation and Experimental Verification of Zn–Al–Mg Phase Diagram
Authors: Li Zhao , Li Yuanpeng , Jiang Sheming , Zhang Jie , Liu Xin , Zhang Qifu , Liu Qiuyuan
Published: April 2024

The liquid phase projection diagram, three-dimensional phase diagram, and vertical section diagram of the Zn–x%Al–x%Mg alloy system was calculated using the phase diagram calculation software Pandat. Simultaneously making full use of the self-developed hot-dip galvanizing process simulation machine by China Steel Research produced a 75%Zn–19%Al–6%Mg coating. A method combining phase diagram calculations and experimental verification was used to investigate the equilibrium phases and solidification process of the alloy. The microstructure of the 75%Zn–19%Al–6%Mg coating was studied using scanning electron microscopy and energy dispersive spectrometry. The results indicate that the coating structure consists of primary Al dendrite phase, MgZn2 inter-metallic compound and Zn-rich phase. There is no ternary eutectic structure in the coating structure. Al dendrites grow on the surface of the coating, while there are no Al dendrites on the cross-section. The experimental results strongly concur with the calculated results from the Pandat phase diagram. The solidification sequence of the 75%Zn–19%Al–6%Mg coating is L→L + Al→L + Al + MgZn2→Al + MgZn2 + Zn. The phase diagram guides industrial production significantly, avoiding the waste of transitional materials and zinc caused by small scale trial and error experiments, thus reducing unnecessary production costs. The factory can select a reasonable coating composition designing scheme in the phase diagram, based on the performance requirements of customers for the coating.
Examining the Changes in Problem Behaviours and Communication of Persons with Intellectual and Developmental Disabilities after Transitioning from an Institutional Setting to the Community
Authors: Kostal Kayla , Baranowski Maria , Casiano Hygiea , Cameranesi Margherita , McCombe Lindsay , Shooshtari Shahin
Published: April 2024

Worldwide, the community transition process away from institutions has increased in the past 30–50 years among persons with severe intellectual and developmental disabilities. This process, also known as “deinstitutionalization”, could potentially impact problem behaviors and communication. This study examined the impacts of community transitions on behavioural and communication outcomes in Canadians with intellectual and developmental disabilities. Data were collected using the Comprehensive Health Assessment Program and medical chart reviews. Descriptive, aggregate-level, and individual-level analyses were conducted for 32 adults who transitioned to community living. Descriptive analyses and the McNemar Chi-Square Test were conducted. Following community transitions, the study group experienced a significant decrease in problem behaviours and changes in communication. The study findings suggest that deinstitutionalization could provide effective strategies for addressing problem behaviors and fostering improvements in communication, promoting the well-being and quality of life of persons with intellectual and developmental disabilities.
Heat-Induced Changes in the Physical Properties of a New Premixed Calcium Silicate-Containing Root Canal Sealer: An In Vitro Study
Authors: Ito Ryota , Watanabe Satoshi , Kouno Akira , Yamauchi Shinya , Okiji Takashi
Published: April 2024

This study aimed to examine how heating affects the physical properties of a newly developed premixed calcium silicate-containing sealer (AH Plus Bioceramic Sealer; AHB), in comparison with EndoSequence BC Sealer (ES), AH Plus Jet (AH), and Pulp Canal Sealer. The setting time, flow, and film thickness were tested with or without heating at 100 °C for 30 or 60 s, in accordance with ISO6876:2012 standards. Ultrastructural and elemental analyses were performed with scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). Data were analyzed using one-way analysis of variance (ANOVA) with a Tukey post hoc test (α = 0.05). All sealers exhibited significantly shorter setting time and flow after heating at 100 °C for 30 and 60 s (p < 0.05). After heating, AHB showed a significantly higher film thickness compared to that of the other materials (p < 0.05). None of the tested properties of heat-applied AHB and ES met ISO standards, except the setting time in ES. The SEM/EDS results for AHB and ES were not affected by heating. The detected changes in physical properties can negatively impact the performance of premixed calcium silicate-containing sealers, particularly AHB, when warm vertical compaction is employed.
Can ESG Stocks Be a Safe Haven during Global Crises? Evidence from the COVID-19 Pandemic and the Russia-Ukraine War with Time-Frequency Wavelet Analysis
Authors: Katsampoxakis Ioannis , Xanthopoulos Stylianos , Basdekis Charalampos , Christopoulos Apostolos G.
Published: April 2024

In times of intense economic variability and social turbulence worldwide, this paper aims to examine the existence of transient correlations and interdependencies between the most important MSCI ESG indices worldwide and the most important commodities’ index, economic uncertainty, natural gas, gold, and VIX, in a geographical and social context during two recent crises: the COVID-19 pandemic and the energy crisis due to the Ukrainian war. Using daily data from 3 January 2020 and extending until 23 August 2022, this study applies a wavelet coherence approach to analyze time series co-movements, in order to emphasize all possible combinations’ correlations and achieve more accurate outcomes at any given time and frequency band simultaneously and spontaneously. The results show robust coherence between different geographical areas, time, and frequency bands, indicating both positive and negative correlations with most of the combined ESG indices and other economic indicators. The study suggests that stock indices of leading ESG companies in North America and Europe constitute a safe investment haven during major upheavals and crises, providing a way for investors to manage risk and generate positive returns while contributing to economic sustainability.
Redesign of a Life Cycle Figure Improves Student Conceptions of Ecology and Evolution
Authors: Landin Jennifer M. , Cozart Abigail Janet
Published: April 2024

Life cycle diagrams communicate the developmental life stages of an organism. Design choices may inadvertently communicate additional information about survivorship rates, genetic variation, and microevolutionary change. In this controlled experiment, we randomly assigned one of three life cycle diagrams to 684 college students. Each figure included identical life stages of a fictitious organism’s development but differed in (1) the number of offspring (single or multiple) and (2) layout (cyclical or linear). Each participant could reference the figure when answering questions about organism survival, variation among offspring, and variation between generations. Students scored 28–30% higher on questions about survivorship when the available diagram included multiple offspring. Students scored 19–30% higher on questions about microevolution when the diagram layout was linear. Overall, students who received the figure with a linear layout and multiple offspring earned the highest average score (54.5%, or 3.3 of six questions) on the assessment, while students with the traditional figure (cyclical layout with single offspring) scored the lowest average (26.1%, or 1.6 of six questions). These results suggest that figure design affects student interpretations and may assist student learning about ecology and evolution concepts and common misconceptions.
From Microservice to Monolith: A Multivocal Literature Review
Authors: Su Ruoyu , Li Xiaozhou , Taibi Davide
Published: April 2024

Recently, the phenomenon of switching back from microservice to monolith has increased in frequency, leading to intense debate in the industry. In this paper, we conduct a multivocal literature review to investigate reasoning and key aspects to pay attention to when switching back and analyze other practitioners’ opinions. The results show four cases of switching back from microservice to monolith: Istio control plane, Amazon Prime Video monitoring service, Segment, and InVision. The five main reasons that led to switching back are cost, complexity, scalability, performance, and organization. During the switching back process, six key aspects need to be addressed: (1) stopping the development of more services, (2) consolidating and testing paths, (3) unifying data storage, (4) implementing the message bus principle, (5) giving up diverse techniques, and (6) learning to use modular design principles. As to the practitioners’ opinions, they had mixed views about the switching back phenomenon. However, most thought that switching back required consideration of the actual system situation and principles. These results pave the way for further research and guide researchers and companies through the process of switching back from microservice to monolith.
MW-Scale High-Voltage Direct-Current Power Conversion for Large-Spacecraft Electric Propulsion
Authors: Sarfi Ghazaleh , Beik Omid
Published: April 2024

This paper proposes a megawatt (MW)-scale high-voltage (HV) electrical power-conversion element for large-spacecraft electric propulsion (EP) systems. The proposed scheme is intended for long-term and crewed missions, and it is driven by a nuclear electric propulsion (NEP) that acts as a heat source. The scheme includes (i) A two-rotor generator (TRG), (ii) A rectification stage, and (iii) An isolated dual output DC-DC (iDC2) converter. The TRG is a high-reliability electric machine with two rotors, a permanent magnet rotor (PMR), and a wound field rotor (WFR). The PMR has a fixed flux and hence back-EMF, while the back-EMF due to the WFR is controlled by injecting a direct current (DC) into the WFR winding. The total TRG output voltage, which is the sum of voltages due to the PMR and WFR, is controlled over a prescribed region of spacecraft operation. The output of the TRG is rectified and connected to the input of the iDC2 converter. The iDC2 converter uses a three-winding transformer, where the primary winding is fed from the rectified output of TRG, the secondary winding processes the propulsion power to an electric thruster via a high-voltage DC (HVDC) link and a tertiary winding that is connected to the spacecraft’s low-voltage DC (LVDC) power system. Three controllers are proposed for the system: an HVDC voltage controller, an HVDC current controller that controls the voltage and current processed to the thruster, and an LVDC controller that adjusts the current to the LVDC system. Detailed analytical models for the TRG, iDC2 converter, and controllers are developed and verified via simulations under different conditions. The analytical studies are further validated via results from a laboratory prototype.
Pushing the Boundaries of Solar Panel Inspection: Elevated Defect Detection with YOLOv7-GX Technology
Authors: Wang Yin , Zhao Jingyong , Yan Yihua , Zhao Zhicheng , Hu Xiao
Published: April 2024

During the maintenance and management of solar photovoltaic (PV) panels, how to efficiently solve the maintenance difficulties becomes a key challenge that restricts their performance and service life. Aiming at the multi-defect-recognition challenge in PV-panel image analysis, this study innovatively proposes a new algorithm for the defect detection of PV panels incorporating YOLOv7-GX technology. The algorithm first constructs an innovative GhostSlimFPN network architecture by introducing GSConv and depth-wise separable convolution technologies, optimizing the traditional neck network structure. Then, a customized 1 × 1 convolutional module incorporating the GAM (Global Attention Mechanism) attention mechanism is designed in this paper to improve the ELAN structure, aiming to enhance the network’s perception and representation capabilities while controlling the network complexity. In addition, the XIOU loss function is introduced in the study to replace the traditional CIOU loss function, which effectively improves the robustness and convergence efficiency of the model. In the training stage, the sample imbalance problem is effectively solved by implementing differentiated weight allocations for different images and categories, which promotes the balance of the training process. The experimental data show that the optimized model achieves 94.8% in the highest mAP value, which is 6.4% higher than the original YOLOv7 network, significantly better than other existing models, and provides solid theoretical and technical support for further research and application in the field of PV-panel defect detection.
Improving Low-Frequency Digital Control in the Voltage Source Inverter for the UPS System
Authors: Rymarski Zbigniew
Published: April 2024

Today voltage source inverters (VSIs) operate with high switching frequencies (let us assume higher than 50 kHz) owing to the fast Si (Silicon) or SiC (Silicon Carbide) switching transistors. However, there are some applications, e.g., with slower switches (e.g., IGBT—Isolated Gate Bipolar Transistor) or when lower dynamic power losses are required when the switching frequency is low (let us assume about 10 kHz). The resonant frequency of the output filter is usually below 1 kHz. The measurements of Bode plots of the measurement traces of various microprocessor-controlled VSIs show that in this frequency range, the characteristics of these channels can be simply approximated through two or three switching periods delay. For the high switching frequency, it is not noticeable, but for the low frequency it can cause some oscillations in the output voltage. One of the solutions can be to use the predictor of the measured state variables based on the full-state Luenberger observer or the linear Kalman filter. Both solutions will be simulated in MATLAB/Simulink and the chosen one will be tested in the experimental VSI. The research aims to omit delays in the measurement channels for the low switching frequency by using the predictions for the measured state variables and finally increasing the gains of the controller to decrease the output voltage distortions.
Comparison of Single-Phase Mathematical Models for Solid-State Packed Beds for Thermal Energy Storage
Authors: Coates Thomas , Torres Sevilla Law , Saeed Burhan , Radulovic Jovana
Published: April 2024

This article presents an analytical solution for the evaluation of the thermal performance of packed bed sensible heat storage. The numerical model developed was tested for four different solid storage mediums. The thermal energy equation is solved numerically by deploying the finite difference method. The presented analytical solution is based on a novel mathematical approach. The numerical model was validated using the computer simulation package Comsol Multiphysics v5.3. Our numerical model results are in good agreement with the published experimental data, with an overall difference of ~10%. Hence, the numerical model is an efficient way of evaluating the thermal performance of packed bed thermal energy storage systems compared to other numerical strategies or computer simulation techniques. This proves that the novel analytical model has shown to be a reliable and broadly accurate approach to acquire the thermal performance of sensible heat storage.
Research on the Temperature Variation Law during the Nitrogen Pre-Cooling Process in LNG Unloading Pipelines
Authors: Yang Wengang , Li Xingyu , Gao Wei , Mi Xiaoguang , Zhang Jinya
Published: April 2024

In order to study the temperature change law of a nitrogen pre-cooling LNG unloading pipeline, a three-dimensional numerical simulation of an LNG pipeline with a bellow expansion bend was conducted using Fluent software (2020 R2). This simulation involved progressively controlling the nitrogen injection temperature and flow rate. The results show that increasing the nitrogen flow rate can improve the pre-cooling rate and reduce the top–bottom temperature difference of the pipeline, but there is an optimal value. Under the same nitrogen injection velocity conditions, it was found that smaller pipe diameters result in smaller temperature differences between the top and bottom of the pipeline. However, due to the reduced cooling capacity of the nitrogen injection, this leads to a decrease in the pre-cooling rate. The top–bottom temperature difference of the pipeline is mainly related to the strength of the natural convection in the pipeline. The stronger the natural convection, the greater the temperature difference between the top and bottom. Gr and Gr/Re2 reflect the relative magnitude of the natural convection intensity and forced convection intensity in the pipe. The larger the Gr and Gr/Re2, the stronger the natural convection. Therefore, Gr and Gr/Re2 are positively correlated with the top–bottom temperature difference, and the variation trend of the top–bottom temperature difference can be judged by the values of Gr and Gr/Re2.
The Energy Conversion and Coupling Technologies of Hybrid Wind–Wave Power Generation Systems: A Technological Review
Authors: Wang Bohan , Sun Zhiwei , Zhao Yuanyuan , Li Zhiyan , Zhang Bohai , Xu Jiken , Qian Peng , Zhang Dahai
Published: April 2024

Based on the mutual compensation of offshore wind energy and wave energy, a hybrid wind–wave power generation system can provide a highly cost-effective solution to the increasing demands for offshore power. To provide comprehensive guidance for future research, this study reviews the energy conversion and coupling technologies of existing hybrid Wind–wave power generation systems which have not been reported in previous publications. The working principles of various wind and wave energy conversion technologies are summarised in detail. In addition, existing energy coupling technologies are specifically classified and described. All aforementioned technologies are comprehensively compared and discussed. Technological gaps are highlighted, and future development forecasts are proposed. It is found that the integration of hydraulic wind turbines and oscillating wave energy converters is the most promising choice for hybrid wind–wave power extraction. DC and hydraulic coupling are expected to become mainstream energy coupling schemes in the future. Currently, the main technological gaps include short their operating life, low energy production, limited economic viability, and the scarcity of theoretical research and experimental tests. The field offers significant opportunities for expansion and innovation.
The Impact of Aluminosilicate Additives upon the Chlorine Distribution and Melting Behavior of Poultry Litter Ash
Authors: Maj Izabella , Niesporek Kamil , Matus Krzysztof , Miccio Francesco , Mazzocchi Mauro , Łój Paweł
Published: April 2024

The use of poultry litter (PL) as a sustainable fuel is gaining more attention due to its wide availability and carbon neutrality. However, this type of feedstock is rich in ash and typically contains a high concentration of chlorine (Cl) and alkali elements (Na, K). Therefore, it is likely to cause unwanted issues during combustion and co-combustion, such as chlorine-induced corrosion, ash deposition, and bed agglomeration. In this study, for the first time, the influence of aluminosilicate additives on the above problems of poultry litter was investigated. Three aluminosilicate minerals are under consideration: kaolin, halloysite, and bentonite. Their influence on the chemical composition and meting tendencies of two poultry litter ashes are determined. The investigated ashes, PL1 and PL2, are characterized by different chlorine contents of 6.38% and 0.42%, respectively. The results show that in the case of the chlorine-rich PL1 ash, the additives reduced the chlorine content by up to 45%, resulting in a 3.93% of chlorine in the case of halloysite, 3.48% in the case of kaolin, and 4.25% in the case of bentonite. The additives also positively influenced the shrinkage starting temperature and the deformation temperature of the PL1 ash.
Metabolic Engineering for Efficient Synthesis of Patchoulol in Saccharomyces cerevisiae
Authors: Tao Qiu , Du Guocheng , Chen Jian , Zhang Juan , Peng Zheng
Published: April 2024

Patchoulol is a natural sesquiterpene alcohol with extensive applications in cosmetics and pharmaceuticals. In this study, we first constructed the synthesis pathway of patchoulol in Saccharomyces cerevisiae by expressing the patchoulol synthase PTS gene using the strong promoter GAL1. Afterward, the metabolic flux of the precursor was enhanced by strengthening the mevalonate pathway and balancing the precursor competition pathway, resulting in a 32.74-fold increase in patchoulol production. Subsequently, the supply of acetyl-CoA in yeast was increased by modifying transcriptional regulators and modulating the acetyl-CoA pathway, and the titer of patchoulol reached 155.94 mg/L. Finally, optimization of the fermentation conditions resulted in a titer of 195.96 mg/L in the shake flasks. Further, batch-fed fermentation in a 5 L bioreactor yielded 1.95 g/L. This work accelerated the development of a microbial cell factory for the production of patchoulol.
An Analysis of Polysaccharides from Eight Plants by a Novel Heart-Cutting Two-Dimensional Liquid Chromatography Method
Authors: Wang Haonan , Jin Hongyu , Chai Ruiping , Li Hailiang , Fan Jing , Wang Ying , Wei Feng , Ma Shuangcheng
Published: April 2024

Natural polysaccharides are important active biomolecules. However, the analysis and structural characterization of polysaccharides are challenging tasks that often require multiple techniques and maps to reflect their structural features. This study aimed to propose a new heart-cutting two-dimensional liquid chromatography (2D–LC) method for separating and analyzing polysaccharides to explore the multidimensional information of polysaccharide structure in a single map. That is, the first-dimension liquid chromatography (1D–LC) presents molecular-weight information, and the second-dimension liquid chromatography (2D–LC) shows the fingerprints of polysaccharides. In this 2D–LC system, the size-exclusion chromatography–hydrophilic interaction chromatography (SEC–HILIC) model was established. Coupling with a charged aerosol detector (CAD) eliminated the need for the derivatization of the polysaccharide sample, allowing the whole process to be completed within 80 min. The methods were all validated in terms of precision, linearity, stability, and repeatability. The capability of the new 2D-LC method was demonstrated in determining various species of natural polysaccharides. Our experimental data demonstrated the feasibility of the whole systematic approach, opening the door for further applications in the field of natural polysaccharide analysis.
Fortified Cereal-Based Foodstuffs: Technological, Sensory, and Nutritional Properties
Authors: Tolve Roberta , Simonato Barbara
Published: April 2024

In the wake of the United Nations’ Agenda 2030, a global commitment to advancing well-being, sustainable living, and waste reduction, the spotlight on cereal-based food products with high added value has intensified [...]
Application of X-STRs for Forensic Identification in Mixed DNA Profile: A Case Report
Authors: Vacca Nunzia Ilaria , Mongelli Giacoma , Ceccardi Stefania , Moroni Elisabetta , Agostini Vincenzo
Published: April 2024

Autosomal polymorphisms (STRs) or Y-Chromosome polymorphisms (Y-STRs) are usually used for the study and deconvolution of mixed DNA profiles in forensic genetics, accompanying data interpretation with biostatistical evaluations (e.g., RMP, RMNE, LR). Sometimes, however, some mixed DNA profiles are so complex that autosomal and Y markers are not sufficient for correct discrimination and identification. In this work is reported a robbery case in which the analysis of the polymorphic markers of the X Chromosome (X-STRs) was applied to the mixed profiles obtained from the traces. This falls outside the classic use of the X-STRs. Indeed, the aim of the authors is to encourage the usage of X-STRs not only in parental relationships, but also in pure forensic cases for interpreting complex mixed DNA profiles, since their application in case resolution could be more decisive than autosomal STRs and Y-STRs.
Recent Insights into Glucose-Responsive Concanavalin A-Based Smart Hydrogels for Controlled Insulin Delivery
Authors: Bercea Maria , Lupu Alexandra
Published: April 2024

Many efforts are continuously undertaken to develop glucose-sensitive biomaterials able of controlling glucose levels in the body and self-regulating insulin delivery. Hydrogels that swell or shrink as a function of the environmental free glucose content are suitable systems for monitoring blood glucose, delivering insulin doses adapted to the glucose concentration. In this context, the development of sensors based on reversible binding to glucose molecules represents a continuous challenge. Concanavalin A (Con A) is a bioactive protein isolated from sword bean plants (Canavalia ensiformis) and contains four sugar-binding sites. The high affinity for reversibly and specifically binding glucose and mannose makes Con A as a suitable natural receptor for the development of smart glucose-responsive materials. During the last few years, Con A was used to develop smart materials, such as hydrogels, microgels, nanoparticles and films, for producing glucose biosensors or drug delivery devices. This review is focused on Con A-based materials suitable in the diagnosis and therapeutics of diabetes. A brief outlook on glucose-derived theranostics of cancer is also presented.
On the Quality and Validity of Course Evaluation Questionnaires Used in Tertiary Education in Greece
Authors: Papadogiannis Ilias , Vassilakis Costas , Wallace Manolis , Katsis Athanassios
Published: April 2024

In compliance with national legislation, Greek tertiary education institutions assess educational quality often using a standardized anonymous questionnaire completed by students. This questionnaire aims to independently evaluate various course components, including content organization, instructor quality, facilities, infrastructure, and grading methods. Despite widespread use across universities, the questionnaire’s validity remains unexamined. This study addresses this gap by analyzing 48,000+ questionnaire responses from the University of the Peloponnese (2014–2022), encompassing 68 undergraduate and graduate programs. Confirmatory factor analyses were used to assess the quality of the questionnaire, while exploratory factor analyses were used to assess the dimensions of the tool based on the data. Both analyses reveal shortcomings: confirmatory analysis detects strong correlations between supposedly different factors, and exploratory analysis identifies dimensions inconsistent with the expected structure. These findings question the questionnaire’s quality and the validity of drawn conclusions, while additionally identifying opportunities for reducing the number of questions, which can contribute to increased questionnaire submission rates. Given its common use across Greek universities and its influence on shaping courses, urgent redesigning of the questionnaire for tertiary education evaluation is recommended.
Biostimulants Improve Bulb Yield, Concomitantly Affecting the Total Phenolics, Flavonoids, and Antioxidant Capacity of Onion (Allium cepa)
Authors: Vojnović Đorđe , Maksimović Ivana , Tepić Horecki Aleksandra , Milić Anita , Šumić Zdravko , Žunić Danijela , Adamović Boris , Ilin Žarko
Published: April 2024

In the pursuit of maximizing onion (Allium cepa) yield and quality, farmers often face the challenges of unfavorable ecological conditions and inadequate agronomic practices. Therefore, our two-year study investigated the effects of biostimulants (BTs) of plant growth on bulb yield and the bioactive compounds of directly seeded onion. Four treatments were applied: control (C), seaweed extracts (BT1), humic and fulvic acid (BT2), and Trichoderma spp. (BT3). The results demonstrated a significant increase in bulb yield with BT1 (↑ 18.7%), BT2 (↑ 18.0%), and BT3 (↑ 24.3%). Intriguingly, all BTs markedly reduced phenolic content across both years. Additionally, BT1 and BT3 elevated flavonoid levels (↑ 16.8% and ↑ 16.7%, respectively), while BT2 decreased them (↓ 24.2%). Notably, in 2021, DPPH, FRAP, and ABTS tests indicated a significant reduction in antioxidant capacity compared to C. Our study underscores the important role of BTs in enhancing yield, influencing secondary metabolites and contributing to environmental sustainability in onion cultivation.
Search Engine for Open Geospatial Consortium Web Services Improving Discoverability through Natural Language Processing-Based Processing and Ranking
Authors: Ferrari Elia , Striewski Friedrich , Tiefenbacher Fiona , Bereuter Pia , Oesch David , Di Donato Pasquale
Published: April 2024

The improvement of search engines for geospatial data on the World Wide Web has been a subject of research, particularly concerning the challenges in discovering and utilizing geospatial web services. Despite the establishment of standards by the Open Geospatial Consortium (OGC), the implementation of these services varies significantly among providers, leading to issues in dataset discoverability and usability. This paper presents a proof of concept for a search engine tailored to geospatial services in Switzerland. It addresses challenges such as scraping data from various OGC web service providers, enhancing metadata quality through Natural Language Processing, and optimizing search functionality and ranking methods. Semantic augmentation techniques are applied to enhance metadata completeness and quality, which are stored in a high-performance NoSQL database for efficient data retrieval. The results show improvements in dataset discoverability and search relevance, with NLP-extracted information contributing significantly to ranking accuracy. Overall, the GeoHarvester proof of concept demonstrates the feasibility of improving the discoverability and usability of geospatial web services through advanced search engine techniques.
The Natural History of Retinal Sensitivity Loss in Diabetic Macular Ischemia over One Year Evaluated by Microperimetry
Authors: Tsai Wei-Shan , Thottarath Sridevi , Gurudas Sarega , Zhao Jinzhi , Cheung Chui Ming Gemmy , Yamaguchi Taffeta Ching Ning , Giani Andrea , Pearce Elizabeth , Sivaprasad Sobha
Published: April 2024

Background/Objectives: This one-year prospective observational study, conducted at two centers, aimed to report the natural history of retinal sensitivity (RS) loss in diabetic macular ischemia (DMI). Methods: Patients with stable-treated proliferative diabetic retinopathy (PDR) were recruited if there was evidence of DMI on optical coherence tomography angiography, defined as a foveal avascular zone ≥ 0.5 mm2 or parafoveal capillary dropout ≥ 1 quadrant. The minimal visual acuity required for performing microperimetry (MP) was ≥54 Early Treatment Diabetic Retinopathy Study letters (Snellen equivalent 20/80). The overall RS (oRS) and pointwise sensitivity (PWS) within the 3 × 3 mm macula were assessed at baseline and twelve months. A value <25 decibels (dB) was defined as impaired RS, and a decrease of 2 and 7 dB was regarded as mild and severe loss, respectively. Results: A total of 88 patients (97 eyes) were included. No statistically significant MP changes were detected at one year. However, 10% of the cohort lost oRS ≥ 2 dB, and 73% lost ≥2 dB PWS in ≥5 loci, whereas 1% lost oRS ≥ 7 dB, and 4% lost ≥7 dB PWS in ≥5 loci. The foveola and temporal parafovea were the most vulnerable to severe RS loss. Compared to their counterpart, eyes with baseline oRS ≥ 25 dB had significantly more RS loss in the macula and superior parafovea (55% versus 32% and 53% versus 28%, both p = 0.01). Conclusions: Rather than oRS loss, ≥2 dB loss in PWS in ≥5 loci is a more feasible outcome measure for clinical trials in DMI.
Culture of Human Embryos at High and Low Oxygen Levels
Authors: Konstantogianni Ourania , Panou Theodoros , Zikopoulos Athanasios , Skentou Charikleia , Stavros Sofoklis , Asimakopoulos Byron
Published: April 2024

One of the parameters potentially affecting the in vitro growth of preimplantation embryos is the oxygen concentration in the culture environment. An increased oxygen concentration causes the generation of ROS which in turn can cause damage to the cells and seriously disrupt the embryonic development. Previous studies have assessed oxygen concentrations in the fallopian tubes of several mammals of between 5 and 8%, while the oxygen levels in the uterus were found to be even lower; similar measurements have been confirmed in humans. In addition, studies in mammalian embryos showed that low oxygen concentrations improve embryo development. Multiple studies on the effect of the oxygen concentration on human embryos have been conducted so far with diverse methodologies and objectives. Data from these have been included in three meta-analyses. All meta-analyses indicate the potential benefit in favor of a low oxygen concentration, though data are considered to be of a low methodological quality and further studies are considered necessary. However, based on the existing evidence, it is suggested that a low oxygen concentration should be adopted in the routine of the IVF laboratory, especially in the case of blastocyst culture.
Revision of Surgery for Adolescent Idiopathic Scoliosis: Reasons, Treatments, and Clinical Management with Case Examples
Authors: Lenke Lawrence G. , Lee Veronica , Hassan Fthimnir M.
Published: April 2024

Adolescent idiopathic scoliosis (AIS) is a curvature of the spine that develops in children ages 10–18 and can be attributed to unknown causes. The Lenke AIS classification system provides a template to classify these deformities by curve type paired with recommended operative treatments. Treatment of this patient population has been associated with low complication rates and overall surgical success. Nonetheless, a fraction of patients remain susceptible to revision surgery. This manuscript will focus on the aspects of AIS surgery, highlighting case examples, the different treatment approaches, complication rates, and primary reasons for revision surgery and associated outcomes.