bluuebunny/crossref_metadata_2025 huggingface dataset:
Whose Turn? Chromosome Research and the Study of the Human Genome
Authors: de Chadarevian Soraya
Published: July 2017

A common account sees the human genome sequencing project of the 1990s as a “natural outgrowth” of the deciphering of the double helical structure of DNA in the 1950s. The essay aims to complicate this neat narrative by putting the spotlight on the field of human chromosome research that flourished at the same time as molecular biology. It suggests that we need to consider both endeavors – the human cytogeneticists who collected samples and looked down the microscope and the molecular biologists who probed the molecular mechanisms of gene function – to understand the rise of the human genome sequencing project and the current genomic practices. In particular, it proposes that what has often been described as the “molecularization” of cytogenetics could equally well be viewed as the turn of molecular biologists to human and medical genetics – a field long occupied by cytogeneticists. These considerations also have implications for the archives that are constructed for future historians and policy makers.
Experimental Investigation of the Mechanism of Foaming Agent Concentration Affecting Foam Stability
Authors: Wang Hetang , Li Jia , Wang Zhan , Wang Deming , Zhan Hanhui
Published: July 2017

With the aim of determining the effect of foaming agent concentration (FAC) on foam stability, the half‐life of a selection of typical foaming agents was investigated at different concentrations using the FoamScan® instrument. The surface tension of the bulk solution after foaming was tested using a surface tension meter. The FAC had a significant effect on foam stability at concentrations <1%, and a weak relationship at concentrations >1%. A significant turning point in the plot of foam stability versus FAC indicated maximum foam stability. The concentration at this point was defined as the optimal stability concentration, which is a guide in foam application. The micelles were thermodynamically unstable at low concentrations and degraded into surfactant solution, but were extremely stable at high surfactant concentrations that did not affect the stability of the foam. A turning point was also observed in the plot of surface tension versus FAC, beyond which the surface tension remained constant; the concentration at this point was defined as the no spherical micelle concentration. The influence of FAC on foam stability is explained in terms of mean bubble diameter (dmb) and bubble size distribution.
Biocomposites for interior facades and partitions to improve air quality in new buildings and restorations
Authors: Roig Inma
Published: July 2017

Composite materials are revolutionizing architecture, since they allow the replacement of traditional materials and provide more options allowing higher design freedom to designers. Durability is one of the key factors in the development of composite materials in the construction sector. The use of biocomposites will allow us to improve the indoor air quality of houses and therefore personal health. It will also increase the thermal and acoustic insulation of houses and control the breathability of construction systems.
Sentinel visits in emergency department patients with diabetes mellitus as a warning sign for hyperglycemic emergencies
Authors: Yan Justin W. , Gushulak Katherine M. , Columbus Melanie P. , Hamelin Alexandra L. , Wells George A. , Stiell Ian G.
Published: July 2017

Objectives

Patients with poorly controlled diabetes mellitus may have a sentinel emergency department (ED) visit for a precipitating condition prior to presenting for a hyperglycemic emergency, such as diabetic ketoacidosis (DKA) or hyperosmolar hyperglycemic state (HHS). This study’s objective was to describe the epidemiology and outcomes of patients with a sentinel ED visit prior to their hyperglycemic emergency visit.

Methods

This was a 1-year health records review of patients≥18 years old presenting to one of four tertiary care EDs with a discharge diagnosis of hyperglycemia, DKA, or HHS. Trained research personnel collected data on patient characteristics, management, disposition, and determined whether patients came to the ED within the 14 days prior to their hyperglycemia visit. Descriptive statistics were used to summarize the data.

Results

Of 833 visits for hyperglycemia, 142 (17.0%; 95% CI: 14.5% to 19.6%) had a sentinel ED presentation within the preceding 14 days. Mean (SD) age was 50.5 (19.0) years and 54.4% were male; 104 (73.2%) were discharged from this initial visit, and 98/104 (94.2%) were discharged either without their glucose checked or with an elevated blood glucose (>11.0 mmol/L). Of the sentinel visits, 93 (65.5%) were for hyperglycemia and 22 (15.5%) for infection. Upon returning to the ED, 61/142 (43.0%) were admitted for severe hyperglycemia, DKA, or HHS.

Conclusion

In this unique ED-based study, diabetic patients with a sentinel ED visit often returned and required subsequent admission for hyperglycemia. Clinicians should be vigilant in checking blood glucose and provide clear discharge instructions for follow-up and glucose management to prevent further hyperglycemic emergencies from occurring.
Collateral Scoring on CT Angiogram Must Evaluate Phase and Regional Pattern
Authors: Casault Colin , Al Sultan Abdulaziz S. , Trivedi Anurag , Sohn Sung Il , Qazi Emmad , Bokyo Matthew , Almekhlafi Mohammed , d’Esterre Christopher , Goyal Mayank , Demchuk Andrew M. , Menon Bijoy K.
Published: July 2017

Purpose: We measured anterior cerebral artery (ACA)-middle cerebral artery (MCA) and posterior cerebral artery (PCA)-MCA pial filling on single-phase computed tomography angiograms (sCTAs) in acute ischemic stroke and correlate with the CTA-based Massachusetts General Hospital (MGH) and digital subtraction angiography (DSA)-based American Society of Interventional and Therapeutic Neuroradiology (ASITN) score. Methods: Patients with acute stroke and M1 MCA±intracranial internal carotid artery occlusion on baseline CTA were included. Baseline sCTA was assessed for phase of image acquisition. An evaluator assessed collaterals using the Calgary Collateral (CC) Score (measures pial arterial filling in ACA-MCA and PCA-MCA regions separately), the CTA-based MGH score, and on DSA using the ASITN score. Infarct volumes were measured on 24- to 48-hour magnetic resonance imaging/ computed tomography. Results: Of 106 patients, baseline sCTA was acquired in early arterial phase in 9.9%, peak arterial in 50.7%, equilibrium in 32.4%, early venous in 5.6%, and late venous in 1.4%. Variance in ACA-MCA collaterals explained only 32% of variance in PCA-MCA collaterals on the CC score (Spearman’s correlation coefficient rho [rho]=0.56). Correlation between ACA-MCA collaterals and the MGH score was strong (rho=0.8); correlation between PCA-MCA collaterals and this score was modest (rho=0.54). Correlation between ACA-MCA collaterals and the ASITN score was modest (n=53, rho=0.43); and correlation between PCA-MCA collaterals and ASITN score was poor (rho=0.33). Of the CTA-based scores, the CC Score (Akaike [AIC] 1022) was better at predicting follow-up infarct volumes than was the MGH score (AIC 1029). Conclusion: Collateral assessments in acute ischemic stroke are best done using CTA with temporal resolution and by assessing regional variability. ACA-MCA and MCA-PCA collaterals should be evaluated separately.
Analysis of a Rotating Disk System with Axial Cooling Air
Authors: Jiao Z. , Fu S. , Kawakubo T. , Ohuchida S. , Tamaki H.
Published: July 2017

In this article, a series of simulations of a rotating disk system with air cooling is introduced. These simulations are performed with the realizable k-ε eddy-viscosity model. Computational results illustrate the effect of the cooling air and disk rotation on the temperature of the disk surface. The maximum rotating disk speed reaches about 78300 rpm and the range of Reynolds numbers based on rotation speed is from about 0.7 × 106to 3 × 106. The present work shows that the flow structure in the gap, which is on the opposite side of the cooling air, is rather similar to different cooling air flows. The temperature goes up as the rotating speed increases. The temperature in this gap will first decrease when the cooling air increases under lower mass flow rate. But when the cooling air continues to increase, the temperature will rise up indicating the existence of an optimum value of the cooling flow rate.
Social network and temporal discounting
Authors: BURT RONALD S.
Published: July 2017

For reasons of social influence and social logistics, people in closed networks are expected to experience time compression: The more closed a person's network, the steeper the person's discount function, and the more narrow the expected time horizon within which the person deliberates events and behavior. Consistent with the hypothesis, data on managers at the top of three organizations show network closure associated with a social life compressed into daily contact with colleagues. Further, language in closed networks is predominantly about current activities, ignoring the future. Further still, discount functions employed by executive MBA students show more severe discounting by students in more closed networks. Inattention to the future can be argued to impair achievement, however, I find no evidence across the managers of daily contact diminishing the achievement associated with network advantage. I close with comments on replication and extrapolation to language more generally, within-person variation, and select cognitive patterns (closure bias, end of history, and felt status loss).
Interactions among High-Frequency Traders
Authors: Benos Evangelos , Brugler James , Hjalmarsson Erik , Zikes Filip
Published: July 2017

Using unique transactions data for individual high-frequency trading (HFT) firms in the U.K. equity market, we examine the extent to which the trading activity of individual HFT firms is correlated with each other and the impact on price efficiency. We find that HFT order flow, net positions, and total volume exhibit significantly higher commonality than those of a comparison group of investment banks. However, intraday HFT order flow commonality is associated with a permanent price impact, suggesting that commonality in HFT activity is information based and so does not generally contribute to undue price pressure and price dislocations.
Antarctic snow melt detection based on the synergy of SSM/I and QuikSCAT
Authors: Li Xinwu , Wang Xingdong , Wang Cheng , Zhang Lu
Published: July 2017

Microwave radiometer SSM/I (Special Sensor Microwave Imager) data and scatterometer QuikSCAT (Quick Scatterometer) data have been widely used for near-surface snow melt detection based on their sensitivity to liquid water present in snow. The SSM/I data have high reliability and the QuikSCAT data have high spatial resolution. In order to improve the accuracy of Antarctic near-surface snow melt detection, we propose a new method based on the synergy of SSM/I and QuikSCAT data, i.e. the snow melt physical model incorporates the complementary advantages of both datasets. Based on comparisons with temperature data from three automatic weather stations, the proposed algorithm improved the accuracy of snow melt detection. The algorithm could also be applied to other regions, which would provide further evidence to support its use and additional data to document changes in the Antarctic due to global climate change.
Uh, bueno, em… : Filled pauses as a site of contact-induced change in Boston Spanish
Authors: Erker Daniel , Bruso Joanna
Published: July 2017

There is mounting evidence that the filled pauses that pervade spontaneous speech constitute a rich site of linguistic inquiry. The present study uses a comparative variationist method to explore possible effects of language contact on pause behavior, examining 3810 filled pauses produced by 24 Spanish-speaking residents of Boston, Massachusetts. Interspeaker differences in pause behavior correlate with intensity of contact. Participants who have lived in the United States for a larger fraction of their lives, who use English more frequently, and who do so more proficiently fill pauses differently when speaking Spanish than do those who have spent less time in the contact setting and whose English skills and usage are more restricted. Results show that a greater degree of contact corresponds to increased use of centralized vowels in phonologically filled pauses (i.e., more frequent use of [a(m)] and [ə(m)] at the expense of [e(m)]). This pattern is interpreted as evidence of contact-induced change.
Large changes in the avifauna in an extant hotspot of farmland biodiversity in the Alps
Authors: KORNER PIUS , GRAF ROMAN , JENNI LUKAS
Published: July 2017

Large declines of farmland bird species have been observed in the lowlands of Western Europe, whereas important populations of some of these species have survived in parts of Eastern and Southern Europe and in small areas within Western Europe, e.g. in parts of the Alps. However, such extant hotspots of farmland biodiversity are at risk: The economic and technical developments threaten to erode biodiversity in existing hotspots, potentially repeating the collapse previously observed in Western Europe. We here present changes in the abundance of farmland birds in the Engadin in the Swiss Alps. Farmland birds such as WhinchatSaxicola rubetraand SkylarkAlauda arvensiswere still numerous in 1987/1988 when we first censused the area. During our second census period in 2009/2010, we noticed strong declines of such open country species, while several hedge and tree breeders as well as some species preferring warmer climate increased. We observed a good correlation between the change in the vegetation and in the birds. Both these changes were especially pronounced in areas with a recent agricultural improvement project. Thus, we believe that the change in farmland practices, which affected our mountainous study area much later than the lowlands, and possibly climate change, have led to a profound change in the regional avifauna. Using our data as a case study, we argue that similar, and similarly fast, changes may be on-going or imminent in many other areas with extant important populations of farmland species such as Whinchat and Skylark. Thus, our data add to the repeatedly declared urgency to adjust the advancement of agricultural subsidy systems to better accommodate biodiversity considerations, both in depauperated areas as well as in extant hotspots.
Unsettling Brian Eno'sMusic for Airports
Authors: SZABO VICTOR
Published: July 2017

In the liner notes to his albumAmbient 1: Music for Airports(1978), Brian Eno (1948–) defined Ambient music in contradistinction to Muzak's ‘derivative’ instrumental pop arrangements. Ambient music's historians and critics have often followed Eno by describing Ambient music as an alternative to conventional ‘background’ or ‘programmed’ music for commercial spaces. Such descriptions can be misleading, however, given that Ambient music's dominant mode of reception is selective personal consumption, not public administration. This article investigates the aesthetics of Eno'sAirports, and elucidates the organizing role of the Ambient genre, within their primary reception context of personal recorded music listening. A comparison with The Black Dog'sMusic for Real Airports(2010) shows how Ambient music then and now reflexively affords atmospheric use by translating a sense of physical dwelling and passage into mixed musical moods. By expressing ambivalenceaboutthe reality of airports and air travel, these Ambient records characteristically convey apprehension about the technological administration of human experience – a phenomenon that includes personal recorded music listening.
Assimilation of Ethnic-Religious Minorities in the Netherlands: A Historical-Sociological Analysis of Pre–World War II Jews and Contemporary Muslims
Authors: Tammes Peter , Scholten Peter
Published: July 2017

This article examines what assimilation trajectories were manifest among present-day Mediterranean Muslims and pre–World War II Jews in Dutch society. Alba and Nee conceptualized assimilation in terms of processes of spanning and altering group boundaries, distinguishing between boundary crossing, blurring, and shifting. This study carves out to what extent assimilation processes like boundary crossing, shifting, and blurring had taken place for those two non-Christian minority groups in Dutch society. This research is based on findings of recent (quantitative) empirical research into the assimilation of pre–World War II Jews in the Netherlands and on the collection of comparable research and data for the assimilation of contemporary Mediterranean Muslims. Our study suggests that processes of boundary crossing, such as observance of religious practices and consumption of religious food, and blurring, such as intermarriage, residential segregation, and religious affiliation, are much less advanced for Mediterranean Muslims in the present time. Though several factors might account for differences in boundary-altering processes between pre–World War II Jews and contemporary Mediterranean Muslims such as differences in length of stay in the Netherlands, the secularization process, and globalization, Jewish assimilation might provide us some reflections on assimilation of Mediterranean Muslims. The continuous arrival of Muslim newcomers might affect attitudes and behavior of settled Mediterranean Muslims, while policy to restrict family migration might be insufficient to stimulate Muslims to integrate in Dutch society given the quite negative mutual perceptions, the slow process of residential spreading, the continuation of observance of religious practices, and the low intermarriage rate.
Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands
Authors: Xu Yang , Chang Lingmo , Huang Anliang , Liu Xiaojun , Liu Xinyu , Zhou Hong , Liang Joshua G. , Liang Peng
Published: July 2017

Aberrant expression of TNF family of cytokines has been linked to human diseases, and biologics targeting their signaling have become the best selling drugs globally. However, functional detection with labeled ligands for accurate detection of TNFR family of receptor-expressing target tissues or cell types remains to be developed. Here we show that TNF receptor family members are heat-stable and can be recognized both in vitro and in vivo by their ligands labeled with alkaline phosphatase. Such an approach may be used in lieu of antibodies for the identification of the cell types involved in receptor signaling during disease onset and progression.
Molecular and Physical Mechanisms of Fibrinolysis and Thrombolysis from Mathematical Modeling and Experiments
Authors: Bannish Brittany E. , Chernysh Irina N. , Keener James P. , Fogelson Aaron L. , Weisel John W.
Published: July 2017

Despite the common use of thrombolytic drugs, especially in stroke treatment, there are many conflicting studies on factors affecting fibrinolysis. Because of the complexity of the fibrinolytic system, mathematical models closely tied with experiments can be used to understand relationships within the system. When tPA is introduced at the clot or thrombus edge, lysis proceeds as a front. We developed a multiscale model of fibrinolysis that includes the main chemical reactions: the microscale model represents a single fiber cross-section; the macroscale model represents a three-dimensional fibrin clot. The model successfully simulates the spatial and temporal locations of all components and elucidates how lysis rates are determined by the interplay between the number of tPA molecules in the system and clot structure. We used the model to identify kinetic conditions necessary for fibrinolysis to proceed as a front. We found that plasmin regulates the local concentration of tPA through forced unbinding via degradation of fibrin and tPA release. The mechanism of action of tPA is affected by the number of molecules present with respect to fibrin fibers. The physical mechanism of plasmin action (crawling) and avoidance of inhibition is defined. Many of these new findings have significant implications for thrombolytic treatment.
UCHL1-HIF-1 axis-mediated antioxidant property of cancer cells as a therapeutic target for radiosensitization
Authors: Nakashima Ryota , Goto Yoko , Koyasu Sho , Kobayashi Minoru , Morinibu Akiyo , Yoshimura Michio , Hiraoka Masahiro , Hammond Ester M. , Harada Hiroshi
Published: July 2017

Hypoxia-inducible factor 1 (HIF-1) has been recognized as an important mediator of the reprogramming of carbohydrate metabolic pathways from oxidative phosphorylation to accelerated glycolysis. Although this reprogramming has been associated with the antioxidant and radioresistant properties of cancer cells, gene networks triggering the HIF-1-mediated reprogramming and molecular mechanisms linking the reprogramming with radioresistance remain to be determined. Here, we show that Ubiquitin C-terminal hydrolase-L1 (UCHL1), which we previously identified as a novel HIF-1 activator, increased the radioresistance of cancer cells by producing an antioxidant, reduced glutathione (GSH), through HIF-1-mediated metabolic reprogramming. A luciferase assay to monitor HIF-1 activity demonstrated that the overexpression of UCHL1, but not its deubiquitination activity-deficient mutant (UCHL1 C90S), upregulated HIF-1 activity by stabilizing the regulatory subunit of HIF-1 (HIF-1α) in a murine breast cancer cell line, EMT6. UCHL1 overexpression induced the reprogramming of carbohydrate metabolism and increased NADPH levels in a pentose phosphate pathway (PPP)-dependent manner. The UCHL1-mediated reprogramming elevated intracellular GSH levels, and consequently induced a radioresistant phenotype in a HIF-1-dependent manner. The pharmacological inhibition of PPP canceled the UCHL1-mediated radioresistance. These results collectively suggest that cancer cells acquire antioxidant and radioresistant phenotypes through UCHL1-HIF-1-mediated metabolic reprogramming including the activation of PPP and provide a rational basis for targeting this gene network for radiosensitization.
Hearables: Multimodal physiological in-ear sensing
Authors: Goverdovsky Valentin , von Rosenberg Wilhelm , Nakamura Takashi , Looney David , Sharp David J. , Papavassiliou Christos , Morrell Mary J. , Mandic Danilo P.
Published: July 2017

Future health systems require the means to assess and track the neural and physiological function of a user over long periods of time, and in the community. Human body responses are manifested through multiple, interacting modalities – the mechanical, electrical and chemical; yet, current physiological monitors (e.g. actigraphy, heart rate) largely lack in cross-modal ability, are inconvenient and/or stigmatizing. We address these challenges through an inconspicuous earpiece, which benefits from the relatively stable position of the ear canal with respect to vital organs. Equipped with miniature multimodal sensors, it robustly measures the brain, cardiac and respiratory functions. Comprehensive experiments validate each modality within the proposed earpiece, while its potential in wearable health monitoring is illustrated through case studies spanning these three functions. We further demonstrate how combining data from multiple sensors within such an integrated wearable device improves both the accuracy of measurements and the ability to deal with artifacts in real-world scenarios.
Shift of EMT gradient in 3D spheroid MSCs for activation of mesenchymal niche function
Authors: Jeon Sohee , Lee Ho-Sun , Lee Ga-Young , Park Gyeongsin , Kim Tae-Min , Shin Jihye , Lee Cheolju , Oh Il-Hoan
Published: July 2017

Despite the wide use of mesenchymal stromal cells (MSCs) for paracrine support in clinical trials, their variable and heterogeneous supporting activity pose major challenges. While three-dimensional (3D) MSC cultures are emerging as alternative approaches, key changes in cellular characteristics during 3D-spheroid formation remain unclear. Here, we show that MSCs in 3D spheroids undergo further progression towards the epithelial-mesenchymal transition (EMT), driven by upregulation of EMT-promoting microRNAs and suppression of EMT-inhibitory miRNAs. The shift of EMT in MSCs is associated with widespread histone modifications mimicking the epigenetic reprogramming towards enhanced chromatin dynamics and stem cell-like properties, but without changes in their surface phenotype. Notably, these molecular shifts towards EMT in 3D MSCs caused enhanced stem cell niche activity, resulting in higher stimulation of hematopoietic progenitor self-renewal and cancer stem cell metastasis. Moreover, miRNA-mediated induction of EMT in 2D MSCs were sufficient to mimic the enhanced niche activity of 3D spheroid MSCs. Thus, the molecular hierarchy in the EMT gradient among phenotypically indistinguishable MSCs revealed the previously unrecognized functional parameters in MSCs, and the EMT-enhanced “naïve” mesenchymal state represents an ‘activated mesenchymal niche’ in 3D spheroid MSCs.
TEPAPA: a novel in silico feature learning pipeline for mining prognostic and associative factors from text-based electronic medical records
Authors: Lin Frank Po-Yen , Pokorny Adrian , Teng Christina , Epstein Richard J.
Published: July 2017

Vast amounts of clinically relevant text-based variables lie undiscovered and unexploited in electronic medical records (EMR). To exploit this untapped resource, and thus facilitate the discovery of informative covariates from unstructured clinical narratives, we have built a novel computational pipeline termed Text-based Exploratory Pattern Analyser for Prognosticator and Associator discovery (TEPAPA). This pipeline combines semantic-free natural language processing (NLP), regular expression induction, and statistical association testing to identify conserved text patterns associated with outcome variables of clinical interest. When we applied TEPAPA to a cohort of head and neck squamous cell carcinoma patients, plausible concepts known to be correlated with human papilloma virus (HPV) status were identified from the EMR text, including site of primary disease, tumour stage, pathologic characteristics, and treatment modalities. Similarly, correlates of other variables (including gender, nodal status, recurrent disease, smoking and alcohol status) were also reliably recovered. Using highly-associated patterns as covariates, a patient’s HPV status was classifiable using a bootstrap analysis with a mean area under the ROC curve of 0.861, suggesting its predictive utility in supporting EMR-based phenotyping tasks. These data support using this integrative approach to efficiently identify disease-associated factors from unstructured EMR narratives, and thus to efficiently generate testable hypotheses.
Anti-centromere antibody exhibits specific distribution levels among anti-nuclear antibodies and may characterize a distinct subset in rheumatoid arthritis
Authors: Kuramoto Nobuo , Ohmura Koichiro , Ikari Katsunori , Yano Koichiro , Furu Moritoshi , Yamakawa Noriyuki , Hashimoto Motomu , Ito Hiromu , Fujii Takao , Murakami Kosaku , Nakashima Ran , Imura Yoshitaka , Yukawa Naoichiro , Yoshifuji Hajime , Taniguchi Atsuo , Momohara Shigeki , Yamanaka Hisashi , Matsuda Fumihiko , Mimori Tsuneyo , Terao Chikashi
Published: July 2017

Anti-centromere antibody (ACA) is one of the classical anti-nuclear antibody (ANA) staining patterns. However, characteristics of ACA in comparison with the other ANA patterns and clinical features of ACA-positive subjects have not been elucidated. Here, we examined all ANA patterns by indirect immunofluorescence for 859 rheumatoid arthritis (RA) patients. Together with the ANA data of 9,575 healthy volunteers, we compared distributions of the ANA levels. ACA was the only ANA that demonstrated a definite bimodal distribution of levels. ACA showed significantly higher levels than the other ANA staining patterns in both RA and healthy population (p < 0.0001). ACA-positivity was associated with old age and was observed more in females. We further recruited another cohort of 3,353 RA patients and confirmed the findings. ACA was also associated with Raynaud’s phenomenon (p = 6.8 × 10−11) in RA. As a conclusion, ACA displays a specific ANA staining pattern with a bimodal distribution, and ACA-positive RA may constitute a distinct subset with specific clinical features.
A 4D view on the evolution of metamorphic dehydration reactions
Authors: Bedford John , Fusseis Florian , Leclère Henri , Wheeler John , Faulkner Daniel
Published: July 2017

Metamorphic reactions influence the evolution of the Earth’s crust in a range of tectonic settings. For example hydrous mineral dehydration in a subducting slab can produce fluid overpressures which may trigger seismicity. During reaction the mechanisms of chemical transport, including water expulsion, will dictate the rate of transformation and hence the evolution of physical properties such as fluid pressure. Despite the importance of such processes, direct observation of mineral changes due to chemical transport during metamorphism has been previously impossible both in nature and in experiment. Using time-resolved (4D) synchrotron X-ray microtomography we have imaged a complete metamorphic reaction and show how chemical transport evolves during reaction. We analyse the dehydration of gypsum to form bassanite and H2O which, like most dehydration reactions, produces a solid volume reduction leading to the formation of pore space. This porosity surrounds new bassanite grains producing fluid-filled moats, across which transport of dissolved ions to the growing grains occurs via diffusion. As moats grow in width, diffusion and hence reaction rate slow down. Our results demonstrate how, with new insights into the chemical transport mechanisms, we can move towards a more fundamental understanding of the hydraulic and chemical evolution of natural dehydrating systems.
Methylation profile of a satellite DNA constituting the intercalary G+C-rich heterochromatin of the cut trough shell Spisula subtruncata (Bivalvia, Mactridae)
Authors: García-Souto Daniel , Mravinac Brankica , Šatović Eva , Plohl Miroslav , Morán Paloma , Pasantes Juan J.
Published: July 2017

Tandemly repeated DNAs usually constitute significant portions of eukaryotic genomes. In bivalves, however, repetitive DNAs are habitually not widespread. In our search for abundant repetitive DNAs in trough shells, we discovered a novel satellite DNA, SSUsat, which constitutes at least 1.3% of the genome of Spisula subtruncata. As foreseen by the satellite DNA library hypothesis, we confirmed that this satellite DNA is also present in two other Mactridae species, showing a highly conserved nucleotide sequence together with a dramatic diminution in the number of repeats. Predominantly located at the G + C-rich intercalary heterochromatin of S. subtruncata, SSUsat displays several DNA methylation peculiarities. The level of methylation of SSUsat is high (3.38%) in comparison with bivalve standards and triplicates the mean of the S. subtruncata genome (1.13%). Methylation affects not only the cytosines in CpG dinucleotides but also those in CHH and CHG trinucleotides, a feature common in plants but scarce and without any clear known relevance in animals. SSUsat segments enriched in methylated cytosines partly overlap those showing higher sequence conservation. The presence of a chromosome pair showing an accumulation of markedly under-methylated SSUsat monomers additionally indicates that the methylation processes that shape repetitive genome compartments are quite complex.
Prion strains depend on different endocytic routes for productive infection
Authors: Fehlinger Andrea , Wolf Hanna , Hossinger André , Duernberger Yvonne , Pleschka Catharina , Riemschoss Katrin , Liu Shu , Bester Romina , Paulsen Lydia , Priola Suzette A. , Groschup Martin H. , Schätzl Hermann M. , Vorberg Ina M.
Published: July 2017

Prions are unconventional agents composed of misfolded prion protein that cause fatal neurodegenerative diseases in mammals. Prion strains induce specific neuropathological changes in selected brain areas. The mechanism of strain-specific cell tropism is unknown. We hypothesised that prion strains rely on different endocytic routes to invade and replicate within their target cells. Using prion permissive cells, we determined how impairment of endocytosis affects productive infection by prion strains 22L and RML. We demonstrate that early and late stages of prion infection are differentially sensitive to perturbation of clathrin- and caveolin-mediated endocytosis. Manipulation of canonical endocytic pathways only slightly influenced prion uptake. However, blocking the same routes had drastic strain-specific consequences on the establishment of infection. Our data argue that prion strains use different endocytic pathways for infection and suggest that cell type-dependent differences in prion uptake could contribute to host cell tropism.
Electrophysiological evidence for pre-attention information processing improvement in patients with central hemiplegic after peripheral nerve rewiring: a pilot study
Authors: Li Tie , Hua Xu-Yun , Zheng Mou-Xiong , Zhu Yu-Lan , Qiu Yan-Qun , Shen Yun-Dong , Xu Jian-Guang , Gu Yu-Dong , Xu Wen-Dong
Published: July 2017

Central neurologic injury (CNI) causes dysfunctions not only in limbs but also in cognitive ability. We applied a novel peripheral nerve rewiring (PNR) surgical procedure to restore limb function. Here, we conducted a prospective study to develop estimates for the extent of preattentive processes to cognitive function changes in CNI patients after PNR. Auditory mismatch negativity (MMN) was measured in CNI patients who received the PNR surgery plus conventional rehabilitation treatment. During the 2-year follow-up, the MMN was enhanced with increased amplitude in the PNR plus rehabilitation group compared to the rehabilitation-only group as the experiment progressed, and progressive improvement in behavioural examination tests was also observed. Furthermore, we found a significant correlation between the changes in Fugl-Meyer assessment scale scores and in MMN amplitudes. These results suggested that PNR could affect the efficiency of pre-attention information processing synchronously with the recovery of motor function in the paralyzed arm of the in chronic CNI patients. Such electroencephalographic measures might provide a biological approach with which to distinguish patient subgroups after surgery, and the change in MMN may serve as an objective auxiliary index, indicating the degree of motor recovery and brain cognitive function.
Heterodera schachtii Tyrosinase-like protein - a novel nematode effector modulating plant hormone homeostasis
Authors: Habash Samer S. , Radakovic Zoran S. , Vankova Radomira , Siddique Shahid , Dobrev Petre , Gleason Cynthia , Grundler Florian M. W. , Elashry Abdelnaser
Published: July 2017

The beet cyst nematodeHeterodera schachtiicauses major yield losses in sugar beet. Understanding the interaction betweenH. schachtiiand its host plant is important for developing a sustainable management system. Nematode effectors play a crucial role in initializing and sustaining successful parasitism. In our study, we identified a gene (Hs-Tyr) encoding a tyrosinase functional domain (PF00264). We describeHs-Tyras a novel nematode effector.Hs-Tyris localized in the nematode esophageal gland. Up-regulation of its expression coincided with the parasitic developmental stages of the nematode. SilencingHs-Tyrby RNA interference made the treated nematodes less virulent. When RNAi-treated nematodes succeeded in infecting the plant, developing females and their associated syncytial nurse cells were significantly smaller than in control plants. Ectopically expressing theHs-Tyreffector in Arabidopsis increased plant susceptibility toH. schachtii, but not to the root-knot nematodeMeloidogyne incognita. Interestingly,Hs-Tyrin the plant promoted plant growth and changed the root architecture. Additionally, the expression ofHs-Tyrin Arabidopsis caused changes in the homeostasis of several plant hormones especially auxin and the ethylene precursor aminocyclopropane-carboxylic acid.
Cell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines
Authors: Zhang Guangmei , Asad Sultan , Khromykh Alexander A. , Asgari Sassan
Published: July 2017

The genus Flavivirus contains more than 70 single-stranded, positive-sense arthropod-borne RNA viruses. Some flaviviruses are particularly medically important to humans and other vertebrates including dengue virus (DENV), West Nile virus, and yellow fever virus. These viruses are transmitted to vertebrates by mosquitoes and other arthropod species. Mosquitoes are also infected by insect-specific flaviviruses (ISFs) that do not appear to be infective to vertebrates. Cell fusing agent virus (CFAV) was the first described ISF, which was discovered in an Aedes aegypti cell culture. We found that while CFAV infection could be significantly reduced by application of RNAi against the NS5 gene, removal of the treatment led to quick restoration of CFAV replication. Interestingly, we found that CFAV infection significantly enhanced replication of DENV, and vice versa, DENV infection significantly enhanced replication of CFAV in mosquito cells. We have shown that CFAV infection leads to increase in the expression of ribonuclease kappa (RNASEK), which is known to promote infection of viruses that rely on endocytosis and pH-dependent entry. Knockdown of RNASEK by dsRNA resulted in reduced DENV replication. Thus, increased expression of RNASEK induced by CFAV is likely to contribute to enhanced DENV replication in CFAV-infected cells.
Absence of deformed wing virus and Varroa destructor in Australia provides unique perspectives on honeybee viral landscapes and colony losses
Authors: Roberts John M. K. , Anderson Denis L. , Durr Peter A.
Published: July 2017

Honeybee (Apis mellifera) health is threatened globally by the complex interaction of multiple stressors, including the parasitic mite Varroa destructor and a number of pathogenic viruses. Australia provides a unique opportunity to study this pathogenic viral landscape in the absence of V. destructor. We analysed 1,240A. mellifera colonies across Australia by reverse transcription-polymerase chain reaction (RT-PCR) and next-generation sequencing (NGS). Five viruses were prevalent: black queen cell virus (BQCV), sacbrood virus (SBV), Israeli acute paralysis virus (IAPV) and the Lake Sinai viruses (LSV1 and LSV2), of which the latter three were detected for the first time in Australia. We also showed several viruses were absent in our sampling, including deformed wing virus (DWV) and slow bee paralysis virus (SBPV). Our findings highlight that viruses can be highly prevalent in A. mellifera populations independently of V. destructor. Placing these results in an international context, our results support the hypothesis that the co-pathogenic interaction of V. destructor and DWV is a key driver of increased colony losses, but additional stressors such as pesticides, poor nutrition, etc. may enable more severe and frequent colony losses to occur.
Use of [C4mim]Cl for efficient extraction of caffeoylquinic acids from sweet potato leaves
Authors: Usuki Toyonobu , Onda Shingo , Yoshizawa-Fujita Masahiro , Rikukawa Masahiro
Published: July 2017

Sweet potato, Ipomoea batatas, is a widely cultivated vegetable worldwide. The leaves contain polyphenolic natural products called caffeoylquinic acids (CQAs), which possess biological activities including inhibition of aggregation of amyloid peptides. The present study describes an efficient extraction and isolation procedure for CQAs from sweet potato leaves using a cellulose-dissolving ionic liquid. The results showed that, compared to methanol, use of 1-butyl-3-methylimidazolium chloride ([C4mim]Cl) allowed the extraction of a 6.5-fold greater amount of CQAs. This protocol will enable the efficient extraction of other organic compounds and biopolymers from natural materials.
Dynamics and mechanisms of clonal expansion of HIV-1-infected cells in a humanized mouse model
Authors: Satou Yorifumi , Katsuya Hiroo , Fukuda Asami , Misawa Naoko , Ito Jumpei , Uchiyama Yoshikazu , Miyazato Paola , Islam Saiful , Fassati Ariberto , Melamed Anat , Bangham Charles R. M. , Koyanagi Yoshio , Sato Kei
Published: July 2017

Combination anti-retroviral therapy (cART) has drastically improved the clinical outcome of HIV-1 infection. Nonetheless, despite effective cART, HIV-1 persists indefinitely in infected individuals. Clonal expansion of HIV-1-infected cells in peripheral blood has been reported recently. cART is effective in stopping the retroviral replication cycle, but not in inhibiting clonal expansion of the infected host cells. Thus, the proliferation of HIV-1-infected cells may play a role in viral persistence, but little is known about the kinetics of the generation, the tissue distribution or the underlying mechanism of clonal expansion in vivo. Here we analyzed the clonality of HIV-1-infected cells using high-throughput integration site analysis in a hematopoietic stem cell-transplanted humanized mouse model. Clonally expanded, HIV-1-infected cells were detectable at two weeks post infection, their abundance increased with time, and certain clones were present in multiple organs. Expansion of HIV-1-infected clones was significantly more frequent when the provirus was integrated near host genes in specific gene ontological classes, including cell activation and chromatin regulation. These results identify potential drivers of clonal expansion of HIV-1-infected cells in vivo.
Constructal law analysis of Cl− transport in eyes aqueous humor
Authors: Lucia Umberto , Grisolia Giulia , Astori Maria Rosa
Published: July 2017

Progressive loss-of-vision related to any intraocular disorder is known as glaucoma. Secretion of aqueous humor is physiologically important to provide nutrients and oxygen to the avascular anterior segment and ensuring normal visual function, even if, nowadays reducing the secretory rate to lower intraocular pressure is a major strategy in treating glaucomatous patients. Understanding the mechanisms and regulation of aqueous humor formation is very important to develop possible new approaches to lower intraocular pressure,but today there isn’t any comprehensive model of the regulation of these component in forming aqueous humor. In this paper Construcal law is used to suggest how the Cl− fluxes can determine the water inflow and outflow, and, consequently, how the intraocular pressure is controlled by these fluxes.
Anti-Fouling Double-Skinned Forward Osmosis Membrane with Zwitterionic Brush for Oily Wastewater Treatment
Authors: Ong Chi Siang , Al-anzi Bader , Lau Woei Jye , Goh Pei Sean , Lai Gwo Sung , Ismail Ahmad Fauzi , Ong Yue Seong
Published: July 2017

Despite its attractive features for energy saving separation, the performance of forward osmosis (FO) has been restricted by internal concentration polarization and fast fouling propensity that occur in the membrane sublayer. These problems have significantly affected the membrane performance when treating highly contaminated oily wastewater. In this study, a novel double-skinned FO membrane with excellent anti-fouling properties has been developed for emulsified oil-water treatment. The double-skinned FO membrane comprises a fully porous sublayer sandwiched between a highly dense polyamide (PA) layer for salt rejection and a fairly loose dense bottom zwitterionic layer for emulsified oil particle removal. The top dense PA layer was synthesized via interfacial polymerization meanwhile the bottom layer was made up of a zwitterionic polyelectrolyte brush - (poly(3-(N-2-methacryloxyethyl-N,N-dimethyl) ammonatopropanesultone), abbreviated as PMAPS layer. The resultant double-skinned membrane exhibited a high water flux of 13.7 ± 0.3 L/m2.h and reverse salt transport of 1.6 ± 0.2 g/m2.h under FO mode using 2 M NaCl as the draw solution and emulsified oily solution as the feed. The double-skinned membrane outperforms the single-skinned membrane with much lower fouling propensity for emulsified oil-water separation.
Antidepressant-like effects and cognitive enhancement of Schisandra chinensis in chronic unpredictable mild stress mice and its related mechanism
Authors: Yan Tingxu , He Bosai , Wan Shutong , Xu Mengjie , Yang Huilin , Xiao Feng , Bi Kaishun , Jia Ying
Published: July 2017

The aim of this study was to evaluate whether Schisandra chinensis extract (SCE) administration influences chronic unpredictable mild stress (CUMS)-induced depression and cognitive impairment, and explores underlying mechanisms. Sucrose preference test (SPT) and forced swimming test (FST) were used for assessing depressive symptoms, and Y-maze, Morris water maze were used for evaluating cognition processes. The results showed that CUMS (4 weeks) was effective in producing both depression and memory deficits in mice. Additionally, CUMS exposure significantly decreased brain derived neurotrophic factor (BDNF) levels in hippocampus as indicated by ELISA, immunohistochemistry and immunofluorescence assays, accompanied by down-regulated tyrosine kinase receptor B (TrkB)/cAMP-response element binding protein (CREB)/extracellular signal-regulated kinase (ERK) and phosphatidylinositol 3 kinase (PI3K)/ protein kinase B (AKT)/ glycogen synthase kinase-3β (GSK-3β) signaling pathways. Chronic administration of SCE (600 or 1200 mg/kg, i.g.) significantly prevented all these CUMS-induced behavioral and biochemical alterations. It suggested that SCE could improve the depression-like emotional status and associated cognitive deficits in CUMS mice, which might be mediated by regulation of BDNF levels in hippocampus, as well as up-regulating of TrkB/CREB/ERK and PI3K/AKT/GSK-3β pathways.
Acute ocular hypertension disrupts barrier integrity and pump function in rat corneal endothelial cells
Authors: Li Xian , Zhang Zhenhao , Ye Lijun , Meng Jufeng , Zhao Zhongyang , Liu Zuguo , Hu Jiaoyue
Published: July 2017

Acute ocular hypertension (AOH) frequently compromises corneal endothelial cell (CEC) function in clinical practice. This type of stress induces corneal oedema and a decrease in the corneal endothelial cell density (ECD). The anterior chamber of the right eye of Sprague-Dawley rats was irrigated with Balanced Salt Solution (BSS) for two hours, and the left eye served as a control to determine the time-dependent effects of AOH on endothelial cell morphology and function. The average intraocular pressure (IOP) increased to 82.6 ± 2.3 mmHg (normal range: 10.2 ± 0.4 mmHg) during anterior irrigation. Very soon after initiating irrigation, corneal oedema became evident and the cornea exhibited a significant increase in permeability to FITC-dextran. The peripheral ECD was significantly reduced, and the morphology of CECs became irregular and multiform. The structures of the zonula occludens-1 (ZO-1) and F-actin were severely disrupted. In addtion, Na,K-ATPase exhibited a dispersed expression pattern. Two days after irrigation, obvious CEC proliferation was observed, the ECD recovered to a normal level, and F-actin was dispersed throughout the cytoplasm. Seven days later, the CEC structure and function were nearly normalized. Based on the results obtained using this model, an acute IOP crisis exerts transient deleterious effects on CEC structure and function in rats.