bluuebunny/crossref_metadata_2025 huggingface dataset:
Ovid: Heroides
Authors: Ovid
Published: October 2018

Ovid's Heroides, a collection of twenty-one epistles in elegiac verse, consists of two groups, the first comprising fourteen poems addressed by heroines of mythology to their absent lovers or husbands. In this edition, Professor Knox offers a commentary on seven of these epistles, addressing problems of language and style, and focusing on the relationship of the Heroides to the classic works of Greek and Roman literature on which Ovid bases his representation of these women. In addition, he has included a commentary on the Epistula Sapphus, a separate poem of doubtful authorship which was composed in the manner of Ovid and is believed by many to be by him. The Introduction provides an account of the genre, a survey of language, style and metre, and an outline of the problems concerning the authenticity of parts of the collection.
Re-imagining rotorcraft advanced design
Authors: Sinsay J. D.
Published: October 2018

Advanced design offices have traditionally applied conceptual design techniques based on semi-empirical methods in an attempt to develop an accurate prediction of aircraft designs at the end of the development process. Continuing advances in computer capability and rotorcraft analysis software present an opportunity to re-think conceptual design to include the greater use of physics-based analyses. A roadmap for developing this capability is outlined, taking into account techniques and ideas from Model-Based Systems Engineering, Design Thinking and Multidisciplinary Optimisation. Recent activities that demonstrate some of these desired capabilities are briefly described along with lessons learned.
Internal wave energy flux from density perturbations in nonlinear stratifications
Authors: Lee Frank M. , Allshouse Michael R. , Swinney Harry L. , Morrison Philip J.
Published: October 2018

Internal gravity wave energy contributes significantly to the energy budget of the oceans, affecting mixing and the thermohaline circulation. Hence it is important to determine the internal wave energy flux $\boldsymbol{J}=p\,\boldsymbol{v}$, where $p$ is the pressure perturbation field and $\boldsymbol{v}$ is the velocity perturbation field. However, the pressure perturbation field is not directly accessible in laboratory or field observations. Previously, a Green’s function based method was developed to calculate the instantaneous energy flux field from a measured density perturbation field $\unicode[STIX]{x1D70C}(x,z,t)$, given a constant buoyancy frequency $N$. Here we present methods for computing the instantaneous energy flux $\boldsymbol{J}(x,z,t)$ for an internal wave field with vertically varying background $N(z)$, as in the oceans where $N(z)$ typically decreases by two orders of magnitude from the pycnocline to the deep ocean. Analytic methods are presented for computing $\boldsymbol{J}(x,z,t)$ from a density perturbation field for $N(z)$ varying linearly with $z$ and for $N^{2}(z)$ varying as $\tanh (z)$. To generalize this approach to arbitrary $N(z)$, we present a computational method for obtaining $\boldsymbol{J}(x,z,t)$. The results for $\boldsymbol{J}(x,z,t)$ for the different cases agree well with results from direct numerical simulations of the Navier–Stokes equations. Our computational method can be applied to any density perturbation data using the MATLAB graphical user interface ‘EnergyFlux’.
Modelling a hydrodynamic instability in freely settling colloidal gels
Authors: Varga Zsigmond , Hofmann Jennifer L. , Swan James W.
Published: October 2018

Attractive colloidal dispersions, suspensions of fine particles which aggregate and frequently form a space-spanning elastic gel are ubiquitous materials in society with a wide range of applications. The colloidal networks in these materials can exist in a mode of free settling when the network weight exceeds its compressive yield stress. An equivalent state occurs when the network is held fixed in place and used as a filter through which the suspending fluid is pumped. In either scenario, hydrodynamic instabilities leading to loss of network integrity occur. Experimental observations have shown that the loss of integrity is associated with the formation of eroded channels, so-called streamers, through which the fluid flows rapidly. However, the dynamics of growth and subsequent mechanism of collapse remain poorly understood. Here, a phenomenological model is presented that describes dynamically the radial growth of a streamer due to erosion of the network by rapid fluid back flow. The model exhibits a finite-time blowup – the onset of catastrophic failure in the gel – due to activated breaking of the inter-colloid bonds. Brownian dynamics simulations of hydrodynamically interacting and settling colloids in dilute gels are employed to examine the initiation and propagation of this instability, which are in good agreement with the theory. The model dynamics is also shown to accurately replicate measurements of streamer growth in two different experimental systems. The predictive capabilities and future improvements of the model are discussed and a stability-state diagram is presented providing insight into engineering strategies for avoiding settling instabilities in networks meant to have long shelf lives.
Familiar faces, familiar spaces: Social similarity and co-presence in non-relational behavioral convergence
Authors: BEHLER RACHEL , SUH CHAN , BRASHEARS MATTHEW , SHI YONGREN
Published: October 2018

Social influence is frequently measured through an ego's direct ties. Although influence may also stem from an ego's indirect ties, reference group, and casual contacts, it is difficult to capture their impact using existing network methods. We identify and trace the influence stemming from an ego's “familiar others,” consisting of those socially similar individuals with whom the ego comes in contact at school, but does not necessarily share a relationship. To evaluate the role of familiar others, we investigate unhealthy weight behaviors in adolescence using data from the National Longitudinal Study of Adolescent to Adult Health. Our results demonstrate that familiar others' unhealthy weight-related behaviors are strong predictors of the ego's own weight behaviors, net of immediate alters' behaviors, and individual-level characteristics. Further, we find that this relationship is stronger and more robust than that between egos and their direct ties. These results suggest that familiar others constitute a key source of social influence that is distinct from the influence of network alters.
Effect of graded calcium supplementation in low-nutrient density feed on tibia composition and bone turnover in meat ducks
Authors: Zhang Huaiyong , Zeng Qiufeng , Bai Shiping , Wang Jianping , Ding Xuemei , Xuan Yue , Su Zhuowei , Zhang Keying
Published: October 2018

Both genetic selection and increasing nutrient density for improving growth performance had inadvertently increased leg problems of meat ducks, which adversely affects animal welfare. We hypothesised that slowing weight gain with improving tibia quality probably enhanced tibial mechanical properties and alleviated leg deformities. Therefore, the present study aimed to evaluate the effect of graded Ca supplementation in a low-nutrient density (LND) diet on tibia composition and bone turnover in meat ducks. A total of 720 15-d-old male meat ducks were randomly assigned and fed a standard nutrient density positive control (PC) diet containing 0·9 % Ca, and four LND diets with 0·5, 0·7, 0·9 and 1·1 % Ca, respectively. Ducks fed the 0·5 % Ca LND diet and the PC diet had higher incidence of tibial dyschondroplasia (TD). When compared with the 0·5 % Ca LND diet, LND diets with ≥0·7 % Ca significantly improved tibia composition, microarchitecture and mechanical properties, and consequently decreased the incidence of TD. Furthermore, LND diets with ≥0·7 % Ca increased osteocyte-specific gene mRNA expression, blocked the expression of osteoblast differentiation marker genes including osteocalcin, collagenase-1 and alkaline phosphatase (ALP), and also decreased the expression of osteoclast differentiation genes, such as vacuolar-type H+-ATPase, cathepsin K and receptor activator of NF-κB. Meanwhile bone markers such as serum ALP, osteocalcin (both osteoblast markers) and tartrate-resistant acid phosphatase (an osteoclast marker) were significantly decreased in at least 0·7 % Ca treated groups. These findings indicated that LND diets with ≥0·7 % Ca decreased bone turnover, which subsequently increased tibia quality for 35-d-old meat ducks.
Moderate coffee consumption is inversely associated with the metabolic syndrome in the Korean adult population
Authors: Kim Youngyo , Je Youjin
Published: October 2018

Results from studies investigating the association between coffee consumption and the metabolic syndrome in Koreans have been inconsistent and remain controversial. We examined coffee intake in relation to the metabolic syndrome using data from the Korea National Health and Nutrition Examination Survey 2012–2015, with 8387 adults aged 19–64 years. Furthermore, we conducted a stratified analysis by obesity status (BMI <25 v. ≥25 kg/m2) to examine whether the association varied by obesity status. A 112-item FFQ was used to assess diet. Multivariable logistic regression models were used to determine the association of coffee consumption (<1, 1–2, 3–4 and ≥5 times/d) and the metabolic syndrome. Compared to people who consumed <1 time/d of coffee, the OR for the metabolic syndrome in those who consumed 3–4 times/d of coffee was 0·75 (95 % CI 0·58, 0·97). However, ≥5 times/d of coffee consumption was not significantly associated with the metabolic syndrome. By obesity status, in non-obese people, those consuming more coffee had lower odds of the metabolic syndrome, showing a significant linear trend (P-trend=0·0248). In obese people, the OR for the metabolic syndrome in people with 3–4 times/d of coffee consumption v. <1 time/d was 0·68 (95 % CI 0·50, 0·93), but ≥5 times/d of coffee consumption showed a non-significant positive association. Our findings suggest that moderate coffee consumption of 3–4 times/d is inversely associated with the metabolic syndrome in Korean adults. The association between heavy coffee consumption of ≥5 times/d and the metabolic syndrome varied by obesity status.
Factors influencing satisfaction with oral contraceptive pills and injectables among past users in Kenya
Authors: Odwe George , Mumah Joyce , Obare Francis , Wamukoya Marylene , Machiyama Kazuyo , Cleland John , Casterline John
Published: October 2018

This study examines factors associated with satisfaction with oral pills and injectables among past users in Kenya based on a baseline survey for the 2-year prospective longitudinal study Improving Measurement of Unintended Pregnancy and Unmet Need for Family Planning conducted in 2016. Married women aged 15–39 years were interviewed using a structured questionnaire that captured information on reproduction, contraceptive knowledge and beliefs and attitudes towards contraception in general and towards specific methods. A multivariate logistic regression analysis was used to examine factors that influenced satisfaction with oral pills and injectables among past users in one urban site (Nairobi slums) and one predominantly rural site (Homa Bay in western Kenya). Results showed that dissatisfaction with pills and injectables is common among past users in both rural and urban Kenya (ranging from 39% to 56%). The distinctive contribution of the study lies in its ability to relate method-specific beliefs to overall satisfaction. Perception of effectiveness, ease of use and safety for long-term use had statistically significant influences on satisfaction with pills in both urban and rural sites while partner’s approval was only important in Nairobi. For injectables, the perception of safety for long-term use was significant in the urban but not the rural site. Unlike pills, the belief that members of a woman’s social network had used a method and found it satisfactory was a particularly powerful influence on satisfaction (AOR=2.8 in rural and 3.2 in urban). Perception of accessibility and fears about infertility were not found to be statistically associated with satisfaction for either pills or injectables. Surprisingly, the effects of all perceived contraceptive attributes were the same for major socio-demographic strata of the populations. The findings underscore the need for targeted counselling and community-based communication interventions to address negative and erroneous perceptions about family planning methods.
When Factors Do Not Span Their Basis Portfolios
Authors: Grinblatt Mark , Saxena Konark
Published: October 2018

To price assets with a parsimonious set of factor-mimicking portfolios, one typically identifies and weights well-diversified basis portfolios. Traditional weightings lead to factor-mimicking portfolios that are unlikely to price even the basis portfolios from which they are formed. We offer a method to combine basis portfolios into a single factor-mimicking portfolio that is closely linked to the optimal portfolio. In practice, this method improves the pricing accuracy of parsimonious factor models, even for anomaly portfolios formed from characteristics that are distinct from those underlying the basis portfolios.
Estimating the incidence of first-episode psychosis using population-based health administrative data to inform early psychosis intervention services
Authors: Anderson Kelly K. , Norman Ross , MacDougall Arlene G. , Edwards Jordan , Palaniyappan Lena , Lau Cindy , Kurdyak Paul
Published: October 2018

Background

Discrepancies between population-based estimates of the incidence of psychotic disorder and the treated incidence reported by early psychosis intervention (EPI) programs suggest additional cases may be receiving services elsewhere in the health system. Our objective was to estimate the incidence of non-affective psychotic disorder in the catchment area of an EPI program, and compare this to EPI-treated incidence estimates.

Methods

We constructed a retrospective cohort (1997–2015) of incident cases of non-affective psychosis aged 16–50 years in an EPI program catchment using population-based linked health administrative data. Cases were identified by either one hospitalization or two outpatient physician billings within a 12-month period with a diagnosis of non-affective psychosis. We estimated the cumulative incidence and EPI-treated incidence of non-affective psychosis using denominator data from the census. We also estimated the incidence of first-episode psychosis (people who would meet the case definition for an EPI program) using a novel approach.

Results

Our case definition identified 3245 cases of incident non-affective psychosis over the 17-year period. We estimate that the incidence of first-episode non-affective psychosis in the program catchment area is 33.3 per 100 000 per year (95% CI 31.4–35.1), which is more than twice as high as the EPI-treated incidence of 18.8 per 100 000 per year (95% CI 17.4–20.3).

Conclusions

Case ascertainment strategies limited to specialized psychiatric services may substantially underestimate the incidence of non-affective psychotic disorders, relative to population-based estimates. Accurate information on the epidemiology of first-episode psychosis will enable us to more effectively resource EPI services and evaluate their coverage.
Crack formation in soda-lime glass after high-power ion beam irradiation
Authors: Kovivchak Vladimir S. , Panova Tatyana V.
Published: October 2018

The fractures of a soda-lime glass surface layer were investigated under irradiation by a high-power ion beam of nanosecond duration with different current densities. Two kinds of cracks (perpendicular and approximately parallel irradiated surfaces) have been identified. It is defined that cracks approximately parallel to the surface are localized at a depth of 10 ± 2 µm from the irradiated glass surface after one-time irradiation with a current density of 100 A/cm2. The formation of these cracks was detected within ~170 h after the end of irradiation when irradiated samples were under an air atmosphere. In a vacuum, the growth of these cracks was insignificant. It is shown that irradiation of heated glass or heating of glass started no later than 2–3 min after irradiation can suppress or substantially reduce surface fracture of glass. The possible mechanisms of glass fracture were discussed.
Racial/ethnic disparities in cortisol diurnal patterns and affect in adolescence
Authors: Deer Lillybelle K. , Shields Grant S. , Ivory Susannah L. , Hostinar Camelia E. , Telzer Eva H.
Published: October 2018

Racial/ethnic minorities are more vulnerable to mental and physical health problems, but we know little about the psychobiological underpinnings of these disparities. In this study, we examined racial/ethnic differences in cortisol diurnal patterns and affect as initial steps toward elucidating long-term health disparities. A racially/ethnically diverse (39.5% White, 60.5% minority) sample of 370 adolescents (57.3% female) between the ages of 11.9 and 18 years (M = 14.65 years, SD = 1.39) participated in this study. These adolescents provided 16 cortisol samples (4 samples per day across 4 days), allowing the computation of diurnal cortisol slopes, the cortisol awakening response, and diurnal cortisol output (area under the curve), as well as daily diary ratings of high-arousal and low-arousal positive and negative affect. Consistent with prior research, we found that racial/ethnic minorities (particularly African American and Latino youth) exhibited flatter diurnal cortisol slopes compared to White youth, F (1, 344.7) = 5.26, p = .02, effect size g = 0.25. Furthermore, African American and Asian American youth reported lower levels of positive affect (both high arousal and low arousal) compared to White youth. Racial/ethnic differences in affect did not explain differences in cortisol patterns, suggesting a need to refine our models of relations between affect and hypothalamic–pituitary–adrenocortical activity. We conclude by proposing that a deeper understanding of cultural development may help elucidate the complex associations between affect and hypothalamic–pituitary–adrenocortical functioning and how they explain racial/ethnic differences in both affect and stress biology.
Agreement of body adiposity index (BAI) and paediatric body adiposity index (BAIp) in determining body fat in Brazilian children and adolescents
Authors: De Santis Filgueiras Mariana , Cecon Roberta Stofeles , de Faria Eliane Rodrigues , de Faria Franciane Rocha , Pereira Patrícia Feliciano , Ribeiro Andréia Queiroz , Priore Silvia Eloiza , de Novaes Juliana Farias
Published: October 2018

Objective

To evaluate agreement of the body adiposity index (BAI) and paediatric body adiposity index (BAIp) in estimating body fat compared with dual-energy X-ray absorptiometry (DXA) and to propose cut-off points for these indices to classify excess adiposity in Brazilian children and adolescents.

Design

Cross-sectional study. Measures of weight, height, hip circumference, BMI and body fat percentage (%BF) assessed by DXA were taken, and BAI and BAIp were calculated. The Bland–Altman plot was used to estimate agreement between the methods, and the receiver-operating characteristic curve to determine the cut-off points for BAI and BAIp per age and sex in comparison with DXA.

Setting

Viçosa, Minas Gerais, Brazil.

Subjects

Children and adolescents aged 8–19 years (n 1049).

Results

Of the children and adolescents, 52·4 % were girls. BAI and BAIp had satisfactory performance by the receiver-operating characteristic curve, except for the 18–19 years age group, whose BAIp had better predictive capacity than BAI. The agreement analysis showed that BAI overestimated %BF by 2·64 %, on average, using DXA; while BAIp underestimated %BF by 3·37 %.

Conclusions

BAI and BAIp showed low agreement with the body fat obtained by DXA, requiring caution when interpreting body composition data in children and adolescents.
Flüchtlingskinder verändern Kitas
Authors: Lorenz Sigrid , Wertfein Monika , Danay Erik
Published: October 2018

Zusammenfassung. Die Aufnahme der Kinder von Schutz- und Asylsuchenden bedeutet für Kindertageseinrichtungen in der Regel eine Auseinandersetzung mit Neuem. Basierend auf einer Online-Befragung in bayerischen Kindertageseinrichtungen und im Rückbezug auf das theoretische Konzept der „Fortschrittsfähigen Organisation“ wird der Frage nachgegangen, in welchem Ausmaß die Aufnahme dieser Kinder von den pädagogischen Fachkräften als Herausforderung und Veränderung ihrer Situation erlebt wird. Die Ergebnisse zeigen eine große Varianz in der Beurteilung nach Art und Stärke, wobei der Umgang mit den Eltern der Flüchtlingskinder häufig herausfordernder erlebt wird als der Umgang mit den Kindern. Darüber hinaus kommt hier bestimmten Teameigenschaften eine signifikante Bedeutung zu, insbesondere dem Vorbereitungsgrad des Teams, seiner Offenheit und seiner Migrationsexpertise.
Differences in Characteristics and Exposure to Stressors Between Persons With and Without Diagnosed Mental Illness Who Died by Suicide in Victoria, Australia
Authors: Clapperton Angela , Newstead Stuart , Bugeja Lyndal , Pirkis Jane
Published: October 2018

Abstract. Background: Mental illness is an established risk factor for suicide. To develop effective prevention interventions and strategies, the demographic characteristics and stressors (other than, or in addition to, mental illness) that can influence a person's decision to die by suicide need to be identified. Aim: To examine cases of suicide by the presence or absence of a diagnosed mental illness (mental illness status) to identify differences in factors associated with suicide in the groups. Method: Logistic regression analyses were used to investigate mental illness status and exposure to stressors among 2,839 persons who died by suicide in Victoria, Australia (2009–2013), using the Victorian Suicide Register. Results: Females, metropolitan residents, persons treated for physical illness/injury, those exposed to stressors related to isolation, family, work, education, and substance use and those who had made a previous suicide attempt had increased odds of having a diagnosed mental illness. Employed persons had decreased odds of having a diagnosed mental illness. Limitations: The retrospectivity of data collection as well as the validity and reliability of some of the data may be questionable owing to the potential for recall bias. Conclusion: The point of intervention for suicide prevention cannot always be a mental health professional; some people who die by suicide either do not have a mental illness or have not sought help.
Discovery of several novel, widespread, and ecologically distinct marine Thaumarchaeota viruses that encode amoC nitrification genes
Authors: Ahlgren Nathan A , Fuchsman Clara A , Rocap Gabrielle , Fuhrman Jed A
Published: October 2018

Much of the diversity of prokaryotic viruses has yet to be described. In particular, there are no viral isolates that infect abundant, globally significant marine archaea including the phylum Thaumarchaeota. This phylum oxidizes ammonia, fixes inorganic carbon, and thus contributes to globally significant nitrogen and carbon cycles in the oceans. Metagenomics provides an alternative to culture-dependent means for identifying and characterizing viral diversity. Some viruses carry auxiliary metabolic genes (AMGs) that are acquired via horizontal gene transfer from their host(s), allowing inference of what host a virus infects. Here we present the discovery of 15 new genomically and ecologically distinct Thaumarchaeota virus populations, identified as contigs that encode viral capsid and thaumarchaeal ammonia monooxygenase genes (amoC). These viruses exhibit depth and latitude partitioning and are distributed globally in various marine habitats including pelagic waters, estuarine habitats, and hydrothermal plume water and sediments. We found evidence of viral amoC expression and that viral amoC AMGs sometimes comprise up to half of total amoC DNA copies in cellular fraction metagenomes, highlighting the potential impact of these viruses on N cycling in the oceans. Phylogenetics suggest they are potentially tailed viruses and share a common ancestor with related marine Euryarchaeota viruses. This work significantly expands our view of viruses of globally important marine Thaumarchaeota.
Astrocytes integrate and drive action potential firing in inhibitory subnetworks
Authors: Deemyad Tara , Lüthi Joel , Spruston Nelson
Published: October 2018

Many brain functions depend on the ability of neural networks to temporally integrate transient inputs to produce sustained discharges. This can occur through cell-autonomous mechanisms in individual neurons and through reverberating activity in recurrently connected neural networks. We report a third mechanism involving temporal integration of neural activity by a network of astrocytes. Previously, we showed that some types of interneurons can generate long-lasting trains of action potentials (barrage firing) following repeated depolarizing stimuli. Here we show that calcium signaling in an astrocytic network correlates with barrage firing; that active depolarization of astrocyte networks by chemical or optogenetic stimulation enhances; and that chelating internal calcium, inhibiting release from internal stores, or inhibiting GABA transporters or metabotropic glutamate receptors inhibits barrage firing. Thus, networks of astrocytes influence the spatiotemporal dynamics of neural networks by directly integrating neural activity and driving barrages of action potentials in some populations of inhibitory interneurons.
SMYD2 glutathionylation contributes to degradation of sarcomeric proteins
Authors: Munkanatta Godage Dhanushka N. P. , VanHecke Garrett C. , Samarasinghe Kusal T. G. , Feng Han-Zhong , Hiske Mark , Holcomb Joshua , Yang Zhe , Jin Jian-Ping , Chung Charles S. , Ahn Young-Hoon
Published: October 2018

Reactive oxygen species (ROS) contribute to the etiology of multiple muscle-related diseases. There is emerging evidence that cellular stress can lead to destabilization of sarcomeres, the contractile unit of muscle. However, it is incompletely understood how cellular stress induces structural destabilization of sarcomeres. Here we report that glutathionylation of SMYD2 contributes to a loss of myofibril integrity and degradation of sarcomeric proteins mediated by MMP-2 and calpain 1. We used a clickable glutathione approach in a cardiomyocyte cell line and found selective glutathionylation of SMYD2 at Cys13. Biochemical analysis demonstrated that SMYD2 upon oxidation or glutathionylation at Cys13 loses its interaction with Hsp90 and N2A, a domain of titin. Upon dissociation from SMYD2, N2A or titin is degraded by activated MMP-2, suggesting a protective role of SMYD2 in sarcomere stability. Taken together, our results support that SMYD2 glutathionylation is a novel molecular mechanism by which ROS contribute to sarcomere destabilization.
Structure of MHC class I-like MILL2 reveals heparan-sulfate binding and interdomain flexibility
Authors: Kajikawa Mizuho , Ose Toyoyuki , Fukunaga Yuko , Okabe Yuki , Matsumoto Naoki , Yonezawa Kento , Shimizu Nobutaka , Kollnberger Simon , Kasahara Masanori , Maenaka Katsumi
Published: October 2018

The MILL family, composed of MILL1 and MILL2, is a group of nonclassical MHC class I molecules that occur in some orders of mammals. It has been reported that mouse MILL2 is involved in wound healing; however, the molecular mechanisms remain unknown. Here, we determine the crystal structure of MILL2 at 2.15 Å resolution, revealing an organization similar to classical MHC class I. However, the α1-α2 domains are not tightly fixed on the α3-β2m domains, indicating unusual interdomain flexibility. The groove between the two helices in the α1-α2 domains is too narrow to permit ligand binding. Notably, an unusual basic patch on the α3 domain is involved in the binding to heparan sulfate which is essential for MILL2 interactions with fibroblasts. These findings suggest that MILL2 has a unique structural architecture and physiological role, with binding to heparan sulfate proteoglycans on fibroblasts possibly regulating cellular recruitment in biological events.
Cryo-EM structures of human STEAP4 reveal mechanism of iron(III) reduction
Authors: Oosterheert Wout , van Bezouwen Laura S. , Rodenburg Remco N. P. , Granneman Joke , Förster Friedrich , Mattevi Andrea , Gros Piet
Published: October 2018

Enzymes of the six-transmembrane epithelial antigen of the prostate (STEAP) family reduce Fe3+ and Cu2+ ions to facilitate metal-ion uptake by mammalian cells. STEAPs are highly upregulated in several types of cancer, making them potential therapeutic targets. However, the structural basis for STEAP-catalyzed electron transfer through an array of cofactors to metals at the membrane luminal side remains elusive. Here, we report cryo-electron microscopy structures of human STEAP4 in absence and presence of Fe3+-NTA. Domain-swapped, trimeric STEAP4 orients NADPH bound to a cytosolic domain onto axially aligned flavin-adenine dinucleotide (FAD) and a single b-type heme that cross the transmembrane-domain to enable electron transfer. Substrate binding within a positively charged ring indicates that iron gets reduced while in complex with its chelator. These molecular principles of iron reduction provide a basis for exploring STEAPs as therapeutic targets.
Anisotropic energy transfer in crystalline chromophore assemblies
Authors: Haldar Ritesh , Jakoby Marius , Mazel Antoine , Zhang Qiang , Welle Alexander , Mohamed Tawheed , Krolla Peter , Wenzel Wolfgang , Diring Stéphane , Odobel Fabrice , Richards Bryce S. , Howard Ian A. , Wöll Christof
Published: October 2018

An ideal material for photon harvesting must allow control of the exciton diffusion length and directionality. This is necessary in order to guide excitons to a reaction center, where their energy can drive a desired process. To reach this goal both of the following are required; short- and long-range structural order in the material and a detailed understanding of the excitonic transport. Here we present a strategy to realize crystalline chromophore assemblies with bespoke architecture. We demonstrate this approach by assembling anthracene dibenzoic acid chromophore into a highly anisotropic, crystalline structure using a layer-by-layer process. We observe two different types of photoexcited states; one monomer-related, the other excimer-related. By incorporating energy-accepting chromophores in this crystalline assembly at different positions, we demonstrate the highly anisotropic motion of the excimer-related state along the [010] direction of the chromophore assembly. In contrast, this anisotropic effect is inefficient for the monomer-related excited state.
Cell shape-independent FtsZ dynamics in synthetically remodeled bacterial cells
Authors: Söderström Bill , Badrutdinov Alexander , Chan Helena , Skoglund Ulf
Published: October 2018

FtsZ is the main regulator of bacterial cell division. It has been implicated in acting as a scaffolding protein for other division proteins, a force generator during constriction, and more recently, as an active regulator of septal cell wall production. FtsZ assembles into a heterogeneous structure coined the Z-ring due to its resemblance to a ring confined by the midcell geometry. Here, to establish a framework for examining geometrical influences on proper Z-ring assembly and dynamics, we sculpted Escherichia coli cells into unnatural shapes using division- and cell wall-specific inhibitors in a micro-fabrication scheme. This approach allowed us to examine FtsZ behavior in engineered Z-squares and Z-hearts. We use stimulated emission depletion (STED) nanoscopy to show that FtsZ clusters in sculpted cells maintain the same dimensions as their wild-type counterparts. Based on our results, we propose that the underlying membrane geometry is not a deciding factor for FtsZ cluster maintenance and dynamics in vivo.
Cathode-enhanced wastewater treatment in bioelectrochemical systems
Authors: Jain Akshay , He Zhen
Published: October 2018

Bioelectrochemical systems (BES) have been intensively studied as a new technology for wastewater treatment. However, the treatment efficiency of BES anodes is limited and the anode effluent usually cannot be directly discharged or reused. To enhance the treatment, BES cathodes may be used for additional treatment of selected contaminants. This has been investigated in a number of approaches, which can be grouped into cathode-stimulated treatment and cathode-supported treatment. The former involves electron transfer directly to reduce contaminants like nitrate or dye compounds, while the latter can accomplish contaminant removal by aerobic oxidation, algal growth, production of strong oxidants for advanced oxidation, and/or membrane treatment. This paper aims to provide a concise view and discussion on the cathode-promoted wastewater treatment in BES, analyze challenges pertaining to the cathode treatment, and offer suggestions on the future development of BES for maximized treatment performance.
Drinking water bacterial communities exhibit specific and selective necrotrophic growth
Authors: Chatzigiannidou Ioanna , Props Ruben , Boon Nico
Published: October 2018

Physicochemical water disinfection methods result in the reduction of bacterial concentrations by orders of magnitude, but not in the total elimination of the bacterial community. As such, the dead bacterial biomass may act as a carbon and nutrient source for the survivor populations. The ability of bacterial strains to grow on dead bacterial cells has been described before as necrotrophy. We investigated the impact of killed bacterial biomass of two different bacterial strains on the growth potential of natural drinking water microbial communities. Many indigenous bacterial taxa could grow on dead biomass, with the total bacterial concentration increasing from 104 to 108 cells/ml. Necrotrophic growth was specific (43 enriched taxa) and selective (i.e. enriched taxa were dependent on the type of dead biomass). The potential of natural water communities to grow necrotrophically has remained underexplored. Nevertheless the phenomenon can have a big impact in water quality and deserves more attention.
A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application
Authors: Lu Yan , Shao Anliang , Shan Yongqiang , Zhao Hongni , Leiguo Ming , Zhang Yongjie , Tang Yinxi , zhang Wei , Jin Yan , Xu Liming
Published: October 2018

Alpha-Gal (Gal) epitopes present in animal tissues are known to be the key xenoantigens that elicit xenorejection. However, a standardized method to determine Gal epitope in animal tissue-derived biomaterials does not exist. Herein, a standardized method for quantitative detection of Gal antigen was established based on an ELISA inhibition assay with Gal antibody. In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible. The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction. This method has been further developed into a detection Kit (Meitan 70101, China), and it has been developed as a standard method for detecting remnant immunogen of animal tissue derived medical devices, and as the industry standard has been released in China. (YY/T 1561–2017).
Topological insulator with negative spin-orbit coupling and transition between Weyl and Dirac semimetals in InGaN-based quantum wells
Authors: Łepkowski S. P. , Bardyszewski W.
Published: October 2018

We study the influence of negative spin-orbit coupling on the topological phase transition and properties of the topological insulator state in InGaN-based quantum wells grown along c axis of the wurtzite lattice. The realistic eight-band k·p method with relativistic and nonrelativistic linear-k terms is employed. Our calculations show that the negative spin-orbit coupling in InN is not an obstacle to obtain the topological insulator phase in InN/InGaN and InGaN/GaN quantum wells. The bulk energy gap in the topological insulator state can reach 2 meV, which allows experimental verification of the edge state transport in these materials. The topological phase transition occurs due to the band inversion between the highest light hole subband and the lowest conduction subband, and almost always is mediated by the two-dimensional Weyl semimetal, arising from an anticrossing of these subbands at zero in-plane wave vector. However, for certain InGaN/GaN quantum wells, we find that the magnitude of this anticrossing vanishes, leading to the appearance of the Dirac semimetal. The novel transition between the Weyl and Dirac semimetals originates from vanishing of the average in-plane spin-orbit interaction parameter, which decouples the conduction subband from the light hole subband at zero in-plane wave vector.
Epidemiology and Clinical Profile of Cutaneous Warts in Chinese College Students: A Cross-Sectional and Follow-Up Study
Authors: Liu Jianjun , Li Hongtian , Yang Fan , Ren Yingyun , Xia Tianbao , Zhao Zigang , Cao Xiaojia , Wang Zekun , Yin Mengmeng , Lu Shichao
Published: October 2018

In this study, the hands and feet of 15,384 undergraduate and postgraduate students in 3 colleges in Beijing were examined for the presence of cutaneous warts at college-entry, and those diagnosed with warts were followed up 2–3 years later. We identified totally 215 (1.4%; 95% CI, 1.2–1.6%) students with warts. The prevalence was significantly higher in male than in female students (2.0%vs. 0.9%,P < 0.0001). Of the 215 patients, 66.9% and 62.1% had only one wart and 98.3% and 93.2% had warts <1 cm in diameter, on the hands and feet, respectively. Of the 130 patients with a follow-up visit, 78 did not receive any treatment (44 recovered within 2 years). Patients aged 21–25 compared to those aged ≤20 were more likely to be free of warts (hazard ratio = 1.76; 95% CI, 1.07–2.89), while lower father’s education (hazard ratio = 0.19; 95% CI, 0.04–0.98) and poor sleep quality (hazard ratio = 0.41; 95% CI, 0.18–0.92) decreased the likelihood of resolution. The prevalence of warts is 1.4% in college students. The majority of patients have warts <1 cm and approximately 2/3 patients has one wart. Slightly over half of patients recover spontaneously within 2 years. Patients’ age, sleep quality, and paternal education may affect the resolution.
Dissociation between the processing of humorous and monetary rewards in the ‘motivation’ and ‘hedonic’ brains
Authors: Chan Yu-Chen , Hsu Wei-Chin , Chou Tai-Li
Published: October 2018

Humor elicits feelings of amusement and can be thought of as a social reward. We identified distinct mesolimbic reward system (MRS) processing patterns for monetary and humorous rewards. During both the reward anticipation and outcome phases, the nucleus accumbens (NAc) and anterior cingulate cortex (ACC) were active in response to monetary cues and monetary gains, while the amygdala and midbrain showed a differential response to humorous rewards, apparently driven by the hedonic enjoyment and appreciation of humor consumption. Psychophysiological interaction analysis (PPI) further demonstrated the functional coupling of theamygdala-midbraincircuit in response to humorous gains during the reward outcome phase, while neural signaling was observed in theNAc-ACCcircuit during both the reward anticipation and outcome phases in response to monetary rewards. This is consistent with a view in which the NAc plays a key role in the ‘motivation brain’, and the amygdala in the ‘hedonic brain’. The findings further suggest that the neural mechanisms underlying reward consumption are more modality-specific than those underlying reward anticipation. Our study contributes to a growing understanding of neural responses to social rewards and represent an important first step toward understanding the neural processing of humor as one significant type of social reward.
Yinchenhao Decoction Alleviates Liver Fibrosis by Regulating Bile Acid Metabolism and TGF-β/Smad/ERK Signalling Pathway
Authors: Cai Fei-Fei , Wu Rong , Song Ya-Nan , Xiong Ai-Zhen , Chen Xiao-Le , Yang Meng-Die , Yang Li , Hu Yuanjia , Sun Ming-Yu , Su Shi-Bing
Published: October 2018

Yinchenhao decoction (YCHD), comprising Yinchenhao (Artemisiae Scopariae Herba), Zhizi (Gardeniae Fructus) and Dahuang (Radix Rhei et Rhizoma), is widely used for treating various diseases. We aimed to investigate the bile acid metabolic mechanism of YCHD in dimethylnitrosamine (DMN)-induced liver fibrosis model. Rats received DMN (10 mg/kg, intraperitoneally) for four successive weeks for liver fibrosis induction and were treated with YCHD for the last 2 weeks. Histopathological analysis showed that YCHD prevented DMN-induced histopathological changes in liver tissues. Serum liver function in YCHD group improved. Ultraperformance liquid chromatography-mass spectrometry analysis showed that YCHD significantly restored both free and conjugated bile acid levels increased by DMN, to normal levels. RT-qPCR results showed that YCHD treatment upregulated the expression of genes related to bile acid synthesis, reabsorption, and excretion. Western blotting analysis showed that YCHD downregulated α-SMA, TGF-β1, p-Smad3, and p-ERK1/2 expression in chenodeoxycholic acid (CDCA)-activated hepatic stellate cells (HSCs). The viability of CDCA-activated HSCs significantly increased after treatment with YCHD and PD98059 (an ERK inhibitor) compared to YCHD treatment alone. Our findings suggest that YCHD alleviated DMN-induced liver fibrosis by regulating enzymes responsible for bile acid metabolism. Additionally, it inhibits CDCA-induced HSC proliferation and activation via TGF-β1/Smad/ERK signalling pathway.
GCSH antisense regulation determines breast cancer cells’ viability
Authors: Adamus Anna , Müller Petra , Nissen Bente , Kasten Annika , Timm Stefan , Bauwe Hermann , Seitz Guido , Engel Nadja
Published: October 2018

Since it is known that cancer cells exhibit a preference for increased glycine consumption, the respective glycine metabolizing enzymes are in focus of many research projects. However, no cancer associated studies are available for the Glycine Cleavage System Protein H (GCSH) to date. Our initial analysis revealed a GCSH-overexpression of the protein-coding transcript variant 1 (Tv1) in breast cancer cells and tissue. Furthermore, a shorter (391 bp) transcript variant (Tv*) was amplified with an increased expression in healthy breast cells and a decreased expression in breast cancer samples. The Tv1/Tv* transcript ratio is 1.0 in healthy cells on average, and between 5–10 in breast cancer cells. Thus, a GCSH-equilibrium at the transcript level is likely conceivable for optimal glycine degradation. A possible regulative role of Tv* was proven by Tv1-Tv*-RNA-binding and overexpression studies which consequently led to serious physiological alterations: decreased metabolic activity, release of the lactate dehydrogenase, increased extracellular acidification, and finally necrosis as a result of impaired plasma membranes. In contrast, Tv1-overexpression led to an additional increase in cellular vitality of the tumor cells, primarily due to the acceleration of the mitochondrial glycine decarboxylation activity. Ultimately, we provide the first evidence of a sensitive GCSH-antisense regulation which determines cancerous cell viability.
Browsing through sealed historical manuscripts by using 3-D computed tomography with low-brilliance X-ray sources
Authors: Stromer Daniel , Christlein Vincent , Martindale Christine , Zippert Patrick , Haltenberger Eric , Hausotte Tino , Maier Andreas
Published: October 2018

Severely damaged historical documents are extremely fragile. In many cases, their secrets remain concealed beneath their cover. Recently, non-invasive digitization approaches based on 3-D scanning have demonstrated the ability to recover single pages or letters without the need to open the manuscripts. This can even be achieved using conventional micro-CTs without the need for synchrotron hardware. However, not all manuscripts may be suited for such techniques due to their material and X-ray properties. In order to recommend which manuscripts and which inks are best suited for such a process, we investigate six inks that were commonly used in ancient times: malachite, three types of iron gall, Tyrian purple, and buckthorn. Image contrast is explored over the complete pipeline, from the X-ray CT scan and page extraction to the virtual flattening of the page image. We demonstrate, that all inks containing metallic particles are visible in the output, a decrease of the X-ray energy enhances the readability, and that the visibility highly depends on the X-ray attenuation of the ink’s metallic ingredients and their concentration. Based on these observations, we give recommendations on how to select the appropriate imaging parameters.
RNA sequencing and de novo assembly of Solanum trilobatum leaf transcriptome to identify putative transcripts for major metabolic pathways
Authors: Lateef Adil , Prabhudas Sudheesh K. , Natarajan Purushothaman
Published: October 2018

Solanum trilobatum L. is an important medicinal plant in traditional Indian system of medicine belonging to Solanaceae family. However, non-availability of genomic resources hinders its research at the molecular level. We have analyzed the S. trilobatum leaf transcriptome using high throughput RNA sequencing. The de novo assembly of 136,220,612 reads produced 128,934 non-redundant unigenes with N50 value of 1347 bp. Annotation of unigenes was performed against databases such as NCBI nr database, Gene Ontology, KEGG, Uniprot, Pfam, and plnTFDB. A total of 60,097 unigenes were annotated including 48 Transcription Factor families and 14,490 unigenes were assigned to 138 pathways using KEGG database. The pathway analysis revealed the transcripts involved in the biosynthesis of important secondary metabolites contributing for its medicinal value such as Flavonoids. Further, the transcripts were quantified using RSEM to identify the highly regulated genes for secondary metabolism. Reverse-Transcription PCR was performed to validate the de novo assembled unigenes. The expression profile of selected unigenes from flavonoid biosynthesis pathway was analyzed using qRT-PCR. We have also identified 13,262 Simple Sequence Repeats, which could help in molecular breeding. This is the first report of comprehensive transcriptome analysis in S. trilobatum and this will be an invaluable resource to understand the molecular basis related to the medicinal attributes of S. trilobatum in further studies.