bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effectiveness of Vaccines in Gurame (Osphronemus goramy) and Challenged Aeromonas hydrophila
Authors: Setyaningsih S U , Kusdarwati R , Rozi  , Handijatno D
Published: February 2020

Gurame (Osphronemus goramy) is one of the most commodities cultivated by the people in Indonesia, but there is a production constraint due to gurame that attacked Motile Aeromonas Septicemia (MAS) disease caused by Aeromonas hyrdophila. One of the most effective precautions that can be used is vaccination. Based on the above description, it is necessary to research the effectiveness of Aeromonas hydrophila vaccine in gurame. This research aims to determine the effect of Aeromonas hydrophila vaccine and the amount of bacterial density in an effective vaccine for preventing MAS on gurame (Osphronemus goramy). This study used an experimental method with a complete lyrandomized design consisting of 5 treatments and 4 replications. Parameters observed were survival rate (SR) and antibody titre of carp. The results of this research indicate that giving Aeromonas hydrophila vaccine to gurame can give an effect for increasing the antibody titer and survival rate (SR). The results showed that the amount of 104 CFU/ml of bacterial density in the Aeromonas hydrophila vaccine was the effective vaccine density used as the MAS prevention and showed a high survival rate of 95%. The conclusion of this research is by giving Aeromonas vaccine can prevent MAS and the density of Aeromonas hydrophila vaccine 104 CFU/ml.
Effects of different feed doses of Majapahit leaves (Crescentia cujete L.) on the growth of Nile tilapia (Oreochromis niloticus)
Authors: Rahmaningsih S , Jumiati  , Awwaliyah S
Published: February 2020

Phytochemical test results of Majapahit leaf extract contain active ingredients saponins, tannins, steroids and triterpenoids which have in vitro potential as antibacterial and from the results of GCMS analysis in previous studies. Majapahit leaves contain active ingredients such as 2-Methoxyl4-vinylpheol, Thiosulfuric acid, Octadec9-enoicacid, n-Hexadeconoic acid which has the potential as an antibacterial insilico. This study aims to determine the effect of the addition of Majapahit leaf powder (Crescentiacujete L.) in feed with different doses to the growth of tilapia (Oreochromis niloticus) The parameters observed in this study were the growth of tilapia weight by treating different doses of majapahit leaf powder with doses A (7.5%), B (10%), C (12.5%) and D (0%). To find out the weight of the fish, sampling was done every 5 days. The results showed the best absolute weight growth in treatment C (12.5%) which was 21.33 g higher than the control treatment which was 10.33 g.
The effect of commercial nutrients to increase the population of Skeletonema costatum on laboratory and mass scales
Authors: Azmi K A , Arsad S , Sari L A
Published: February 2020

S. costatumis one type of phytoplankton used in the maintenance of fish, shrimp, and crab larvae, but most are given in the maintenance of tiger shrimp larvae (Penaeus monodon) from the phase of nauplius phase after zoea.S. costatumhas the advantages found in the autolysis enzyme itself so that it is easily digested by the larvae and does not pollute the cultivation media. Therefore knowledge about how is the effect of nutrients inS. costatumcommercial life of food culture on laboratory scale up to mass scale. The methodS. costatumis generally started by sterilizing tools and materials, preparation of culture media for laboratory scale (2 liters), semi-mass scale (80 liters) and mass scale (2 tons), fertilizer application, seed selection, the cost of countingS. costatumcells with peak population depends on the 36th hour with the highest number of cells in each culture scale items, namely laboratory scale of 602.547 × 103cells/mL, semi-mass scale of 484.713 × 103cells/mL and mass scale of 133.757 × 103 cells/mL then the last process is harvesting until it becomes flour. The obstacles encountered are environmental problems such as unstable weather and can be contaminated.
Cultivation technique of Chanos chanos modular system and semi intensive at the center for brackish water aquaculture (BBPBAP) Jepara, Central of Java
Authors: Nisa M , Kismiyati K
Published: February 2020

Milkfish (Chanos chanos) is a commodity that has the highest increase in consumption value, which is 24.55%. Milkfish production centers in Indonesia have developed such as in East Java, South Sulawesi, West Java, Central Java, and Southeast Sulawesi (DJPB, 2015). The technology of enlargement and seeding has been mastered and developed in the community, for example modular, semi-intensive and intensive systems. The difference in the growth of modular and semi-intensive milkfish systems has been observed in the Center for Brackish Water Aquaculture (BBPBAP) Jepara, Central Java on 19 December 2018 until 19 January 2019. Since 1 month, milkfish was observed with long, heavy growth parameters, life velocity of milkfish, and water quality. Milkfish enlargement techniques are preparation of ponds, liming, fertilization, stocking of milkfish logs, management of water quality, pest and disease control, and harvesting. Based on observations made, the growth in length and weight of milkfish develops faster using semi-intensive systems compared to modular systems. The semi-intensive system also speeds up the harvest of milkfish, which are generally 6 months to 4 months.
Effect of Spirulina platensis supplementation in the diet to sperm performance of silver rasbora (Rasbora argyrotaenia)
Authors: Putri M W D , Prayogo  , Budi D S
Published: February 2020

Silver rasbora (Rasbora argyrotaenia) is a fish that high economic price, a relatively short maintenance period, and cultivation can be in a narrow area. The purpose of this research was to know the effect and optimum dosage of S. platensis supplementation in diet to sperm performance of silver rasbora. This study was conducted in February until March 2019 at Wet Laboratory and Instrument Laboratory Universitas Airlangga, Banyuwangi Campus, Indonesia. A total 24 male of silver rasbora with initial weight of 3.96 ± 0.41 g and length of 7.58 ± 0.41 cm was randomly distributed in 4 tanks (6 fish per tank) according to 4 different treatment doses of S. platensis in the diet namely 0% (P0), 1% (P1), 2% (P2), and 3% (P3). The parameters were observed in this study are milt volume, sperm concentration, sperm motility, and sperm viability. Supplementation of S. platensis in diet was increased sperm performance of silver rasbora and the optimum dosage is 3% with the amount of milt volume, sperm motility, sperm concentration, sperm viability are 9.11 ± 0.95 μL/gr body weight, 76.07 ± 0.97 %, 82.70 ± 0.79 ×104 cell/μL, and 73.90 ± 1.8 %.
Antibacterial activity test of mahkota dewa leaf extract (Phaleri amacrocarpa) against bacteria Aeromonas hydrophilla by in vitro
Authors: Sarendah R A , Sudarno  , Kusdarwati R
Published: February 2020

Disease motile aeromonas septicemia (MAS) caused by the bacteriumA. hydhrophilla, is a disease that can affect all species of freshwater fish in the tropics. The use of antibiotics for the treatment of bacterial diseases have long been used, but the use of antibiotics that are not controlled pathogens causing bacterial resistance. The purpose of this study to determine the effect and the optimum concentration of the mahkota dewa leaf extract against bacteriaA. hydhrophilla. The treatment in this study was the addition of the mahkota dewa leaf extract and DMSO with a variant concentration of 100 mg, 140 mg, 180 mg, 220 mg, 260 mg, positive control using antibiotics chloramphenicol and negative controls using DMSO. Research of mahkota dewa leaf extracts to inhibit the growth ofAeromonas hydrhopilla. This is indicated resources by the formation of an inhibition zone at all concentrations. The formation of inhibition zone of the mahkota dewa againstA. hydhrophillaleaf extract shows that the mahkota dewa leaf extract can inhibit the growth of bacteria.
Nile tilapia (Oreochromis niloticus) fish hatchery technique: the survival rate evaluation in IBAT Pandaan, Pasuruan, East Java
Authors: Putri T A , Maya S , Santanumurti M B
Published: February 2020

Nile tilapia (Oreochromis niloticus) is a freshwater fish that has great potential to be developed and has a fairly high economic value. One of the important factors to support the success of Tilapia fish farming is the availability of seeds. Instalasi Budidaya Air Tawar (IBAT) Pandaan is contributing to the fulfillment of the superior seeds through the activities of tilapia fish hatchery. The purpose of this study was to identified the performance of hatcheries techniques of tilapia fish in Instalasi Budidaya Air Tawar (IBAT) Pandaan, Pasuruan, East Java through the survival rate. This research used in this study was descriptive method, supported by primer and secondary data. The survival rate result showed that high value of survivaval rate of 92% and showed good condition of fish This was due to the good technique of tilapia hatcheries included the pond preparation, broodstock selection and preparation, hatchery, feeding, and water quality control.
The effect of concrete tanks for the breeding technique of the sand sea cucumber (Holothuria scabra)
Authors: Sitoresmi D A N , Pursetyo K T
Published: February 2020

Sea cucumber are one of the Echinodermata type that have nutritional value in high protein content and as the sea of commodities with high economical value. The attempts to Obtain sea cucumber are Increasingly declining. Based on the review above, it is noticed that the cultivation of sea cucumber are needed to fulfill the needs of the market and restore the wild population. Sand sea cucumber breeding begins with the preparation of the broodstock rearing media, the availability preparation of the broodstock, water quality management, the broodstock feeding, the broodstock selection for high quality broodstock, conducting the artificial spawning using the method of temperature shock, egg incubation, hatching egg, and the larval rearing. The larvae were fed using the phytoplanktonChaetocerossp. with a feeding frequency of once per day. The water quality parameters measured include temperature, DO, pH and salinity. The obstacles that were found on the sand sea cucumber breeding is the difficulty of getting the high quality of the broodstock, so the egg produced from spawning process are limited.
The effect of various concentration of quail egg yolk on spermatozoa motility of kancra fish (Tor soro Valenciennes, 1842) post cryopreservation
Authors: Laeni M , Abinawanto  , Subagja J , Kristanto A H
Published: February 2020

Kancra fish, also known as Batak fish, is often used in North Sumatera in traditional ceremonies. Nevertheless, overfishing has led to a decrease in its population. Cryopreservation is a strategy that might be effectively used to solve this problem. This study aimed to evaluate the effect of various concentrations of quail egg yolk on the spermatozoa motility of kancra fish after 48-hours of cryopreservation. The concentration of quail egg yolk were 0%, 5%, 10%, 15%, 20%, and 25%. The sperm of kancra fish were collected using the stripping method and was diluted with the quail egg yolk, fish ringer, and 10% methanol. It was then equilibrated for 10 minutes at a temperature of 5° C before being frozen at -10 °C for 48 hours. Thawing was carried out at 40 °C for 1 minute. The motility was analyzed using ANOVA and proceeded by Tukey test. The ANOVA test showed the use of various concentrations of quail egg yolk has a significant effect (p<0.05) on the spermatozoa motility of kancra fish after cryopreservation. The concentration of 10% of quail egg yolk showed the highest spermatozoa motility (85.10 ± 1.51%) after 48-hours of cryopreservation (p<0.05).
The spermatozoa motility of kancra fish (Tor soro Valenciennes, 1842) after the frozen process: the application of egg yolk as a cryoprotectant
Authors: Vardini N , Abinawanto  , Subagja J , Kristanto A H
Published: February 2020

Kancra is one of the endemic fishes in Indonesia, however, its population keeps decreasing due to overexploitation. One of the ways methods to solve this problem is by implementing cryopreservation. Cryoprotectant has effect on successful cryopreservation. The purpose of this study was to evaluate the effect of free-range chicken egg yolk as a natural cryoprotectant combined with 10% of methanol to motility of spermatozoa that had been used after 48 hours cryopreservation. The egg yolk concentration used were 0%, 5%, 10%, 15%, 20%, and 25%. The sperm was collected by stripping and diluted with methanol and fish ringer, then it was equilibrated at 5 °C for 10 minutes. After that, it was frozen at -10°C for 48 hours and thawed at 40 °C for 1 minute. The spermatozoa motility was analyze using ANOVA and Tukey test. The results show there is a significant effect (p <0.05) on all of egg yolk concentrations. The 5% concentration of free-range chicken egg yolk shows the highest motility percentage of 84.06 ± 1.67%.
Sperm motility of kancra fish (Tor soro, Valenciennes 1842) after frozen: the effect of soybean milk as a natural cryoprotectant
Authors: Fatriani R , Abinawanto  , Arifin O Z , Kristanto A H
Published: February 2020

Sperm cryopreservation is a way of managing and maintaining kancra fish (Tor soro) resources that increasingly threatened because of over-exploitation. The optimization of sperm cryopreservation is carried out using a natural cryoprotectant with lower toxicity than a chemical cryoprotectant. The purpose of this study was to analyze the effect of soybean milk as a natural cryoprotectant on sperm motility percentage of kancra fish after 48 hours preservation. The sperm was collected through stripping and was diluted into the soluble liquid of fish ringer + methanol 10% + soybean milk. Importantly, the concentration of soybean used were 0%, 5%, 10%, 15%, 20%, and 25%. The sample was equilibrated at the temperature of 5 °C for 10 minutes, frozen at the -10 °C for 48 hours and thawed at 40 °C for 1 minute. The motility percentage was analyzed using ANOVA and Tukey test. The result showed soybean milk had effects (p < 0.05) on the post-cryopreservation sperm motility. Additionally, 5% of soybean milk showed the highest sperm motility (84.37 ± 1.54%).
Prevalence and intensity of protozoan ectoparasite infestation on nursery of humpback grouper (Cromileptes altivelis) in hatchery and floating net cage
Authors: Mahasri G , Subekti S , Angghara B B , Pratama F P
Published: February 2020

Ectoparasites which often attack humpback groupers are protozoa from the Ciliate class, Trichodina sp. and Cryptocaryon sp. This study studied the differences in the prevalence and intensity of protozoan ectoparasites in nursery of humpback grouper in hatcheries and floating net cages. The results showed that there was a significant difference (p <0.05) between the prevalence and intensity of protozoan ectoparasites in nursery of grouper which were kept in hatchery and floating net cages. The prevalence nursery of humpback grouper infested from hatcheries and floating net cages is Trichodina sp. ectoparasites are 83.3% and 76.6%. Prevalence of Cryptocaryon sp. ectoparasites are 76.6% in hatchery and the prevalence of floating net cages is 50%. From the statistical test shows that the intensity of the Trichodina sp. ectoparasites at the hatchery is 12.76 individuals/head was significantly different (p <0.05) on the intensity of the Trichodina sp. ectoparasites. In floating net cages of 4.2 individuals/head. Meanwhile, the results of statistical tests on the intensity of ectoparasite Cryptocaryon sp. at the hatchery is 4.47 individuals/head significantly different (p <0.05) on the intensity of ectoparasites Cryptocaryon sp. in floating net cages there are 1.73 individuals/head.
Growth of Cantang Hybrid Grouper Juvenile (Epinephelus fuscoguttatus x Epinephelus lanceolatus) With Different Feeding Frequency
Authors: Nuraini D , Agustono  , Lutfiyah L
Published: February 2020

Grouper is one of the fish that is much sought after by the general public because grouper have high economic value and one of the exported fish products. The high demand for grouper to cause community develop hatcheries grouper. Appropriate feeding and efficiency very influential towards the process of optimal fish growth. One of the feeding management of frequently used IE frequency feeding. Feed frequency is influenced by several factors, namely age, type and the appetite of the fish itself. The purpose of this study is to see the number of frequency of feeding on seeds of hybrid grouper cantang best to promote growth and feed efficiency and to lower the value of the feed conversion. This study used a RCD. The results showed that the granting of different frequency-feed can increase the growth of specific daily on the seed of hybrid cantang grouper. The highest growth rate of treatment weight i.e. P2 of 4.2 %/day. The value of the efficiency of feed conversion ratio and best i.e. P1 of 67% and 1.55. Treatment of the P1 value is the SGR. efficiency of feed conversion ratio and the lowest i.e. respectively 3.3%/day. 46% and 2.26.
The harmful effect of commercial powder detergent on water flea (Daphnia sp.)
Authors: Kenconojati H , Suciyono  , Azhar M H
Published: February 2020

Detergents are a chemical product that is commonly used in personal care and household product on a daily basis. Its huge consumption in human activities resulted in the increasing awareness of its toxicity in the aquatic environment due to their active ingredients. Daphnia is a planktonic invertebrate organism that usually found in freshwater ecosystems. It plays an essential part in the lower trophic level of food chains. Because of its sensitivity to various environmental changes, they are generally used as a model organism in toxicology. In the present study, we analyzed the toxic effect of household detergent exposure on Daphnia sp. Four different concentration of detergent (25, 50, 75, and 100 mg.L−1) were prepared and the aquadest was used as control. The mortality of Daphnia was observed for 24 hours. The result showed that the mortality of Daphnia increased along with the increasing concentration of detergent exposures. LC50-24 hours values obtained in this study was 28.89 mg.L−1. This result described that the higher level of detergent in a water body can be harmful to the aquatic organism, especially on Daphnia sp. as predominant food for planktivorous fish.
Addition different algae (Spirulina) flour to artificial feed on color quality and growth of Koi fish (Cyprinus carpio-Koi)
Authors: Sudirman D A , Arief M , Fasya A H
Published: February 2020

Abstrak

Ornamental fish is one of the fisheries commodities that can be used as a source of foreign exchange income, and has its own beauty to attract the interest of ornamental fish lovers. One of the things that determines beauty in many species of ornamental fish is color. Color is a parameter to determine the value of ornamental fish, which is a brighter color, the higher the value of fish. Spirulina sp. Flour has a high carotenoid and protein content, each of which plays a role in increasing color intensity and growth. The purpose of this study was to determine the effect of concentration Spirulina flour to the color quality and growth of koi fish (Cyprinus carpio). The method used was experimental with Completely Randomized Design (CRD) using 4 treatments 5 replications. The dose of feed used is (Control), (dose 1%), (dose 3%) and (dose 5%), Color quality measurement using Adobe Photoshop CS8 software. The frequency of twice-daily feeding for each treatment resulted in color quality with the best value (3.0200%), absolute length growth (0.9780 cm) and absolute weight growth (3.7240 grams).
Tuna fisheries in fisheries management area Republic of Indonesia 572
Authors: Suyasa N , Rahardjo P , Putri D R , Widagdo A
Published: February 2020

This research is expected to improve the sustainable tuna fisheries management of Fisheries Management Area Republic of Indonesia (FMARI) 572 by considering fish resources and fishermen welfare. The objectives of the research are examining the status of tuna fisheries in the FMARI 572, analyzing the bioeconomic model of tuna fisheries in the FMARI 572, and determining the strategy of a sustainable tuna fisheries management in the FMARI 572. This research was carried out for 3 months from February to May 2019 at the existing fishing ports located along the west coast of Sumatra (FMARI 572), such as Lampulo Fishing Port (Aceh), Sibolga Fishing Port (North Sumatra), Bungus Fishing Port (West Sumatra), and Nizam Zachman Fishing Port (Jakarta). Some factors analyzed were: The fishing capacity of fishing units with a variety of fishing gears, the potential of fish resources, the optimum level of efforts reaching the sustainable maximum catches, and the implementation of fisheries bioeconomic models. The results of this study showed the level of utilization tuna in FMARI 572 is still moderate, Tuna fisheries in FMARI 572 have not experienced biological and economical overfishing, and there were 3 sustainable tuna fisheries management strategies proposed.
The relation between quality of the sediment (nitrate, phosphate) and Avicennia sp density, case study; Mangrove Center Bengkak, Banyuwangi Regency, East Java
Authors: Suciyono  , Rahardja B S , Prayoga A I , Kenconojati H , Ulkhaq M F
Published: February 2020

Bengkak mangrove center as a natural environment has been managed by the society as a means of tourism and preservation. These areas were located on Bali strait, consist of fisheries activity and residential. This study aims to determine the density of Avicennia sp, nitrate and phosphate content and the relationship between both. Three stations were determined using purposive sampling and the sample was obtained using the belt transect technique. Linear regression analysis was carried out to determine the density of Avicennia sp, with nitrate, phosphate content. Results of this study showed that the highest density of Avicennia sp was found in station 3 with a value of 3.25, followed by station 2 of 1.38, and the lowest density at station 1 was 0.71. Nevertheless, the highest concentrations of nitrate and phosphate were found at station 3 of 207.27 mg/kg and 0.23%. The relationship between the density of Avicennia sp, with nitrate and phosphate showed a positive correlation.
Screening of antivibrio-producing lactic acid bacteria originated from aquatic animals as probiotic candidates
Authors: Amin M , Liliyanti M A , Nufus N H , Ali M
Published: February 2020

Acute hepatopancreatic necrosis disease has been a major challenge faced by white shrimp farmers in many shrimp-producing countries inducing Indonesia. The etiological agent of this disease was a virulent strain of Vibrio parahaemolyticus. However, a technology to treat or to prevent the disease infection has not been established yet, and probiont could be a potential approach for this disease. Thus, this study aimed at screening 35 lactic acid bacteria (LAB) that originated from the gastrointestinal tract of wild white shrimp for the production of antivibrio compounds using an agar well diffusion. The result showed that three LAB strains (LAB-1, LAB-2, and LAB-3) showed antivibrio activity indicated by the formation of clearance zone (diameter of >10mm) on the diffusion agar plate. When the extracellular products (ECP) were incubated with proteinase K, the antivibrio activity was disappeared which indicated that the antimicrobial compounds were bacteriocin-like inhibitory substances (BLIS). Phenotypic studies suggest that the LAB were Labctobacillus sp. (LAB-1 and LAB-2), and Enterococcus sp. (LB-3). These results suggest these LAB are potential candidates for preventing V. parahaemolyticus. Infection, although, further assays by in vivo using live white shrimp, are still required.
The spatial pattern of turbidity and light transmission around the coastal waters of Cisadane in July and November 2014
Authors: Azis Ismail M F , Prayitno H B
Published: February 2020

We observed the spatial pattern of physical oceanographic parameters, namely seawater turbidity and light transmission around the coastal waters of Cisadane. The observations were taken in July and November 2014 at 18 stations distributed in surrounding coastal waters of Cisadane. Water turbidity and light transmission were measured using CTD SBE 19 censor that could record simultaneously vertical profile of water turbidity and light transmission. The station’s position was determined using a GPS instrument. The results showed that seawater turbidity and light transmission exhibit two distinct spatial and temporal variation which is closely associated with the monsoonal wind system in the region.
Structure communities of macrozoobenthos in mangrove tourism area, Wongsorejo sub-district, Banyuwangi regency, East Java
Authors: Fatmawati D , Rahardja B S , Suciyono  , Lutfiah L , Ulkhaq M F
Published: February 2020

Macrozoobenthos is an organism that lives on the surface or in liquid sediment. Macrozoobenthos is one of the most important organisms for marine and coastal ecosystems. This study aimed to determine the value of the community structure of macrozoobenthos existing in Mangrove Center Bengkak, a subdistrict of Wongsorejo, Banyuwangi regency. Sampling was conducted on three stations with different location characteristics. The community structure was measured by Shannon’s diversity index, Equitability index, and dominance index. The results of this study obtained Shannon’s diversity index of 0.51, equitability index of 0.12, and dominance index of 0.76 at Station 1. At station 2, Shannon’s diversity index of 1.19, equitability index of 0.39, and dominance index of 0.36. While at station 3, it was obtained that Shannon’s diversity index of 1.64, equitability index of 0.85, and dominance index of 0.31.
Social network analysis: Key actors of cooperation between Sama Bajo and land-dwellers in Wakatobi marine national park
Authors: La Ola T , Wianti N I , Tadjuddah M , Suriana  , Buana T , Rosmawaty  , Abdullah S , Wunawarsih I A
Published: February 2020

The relationship between Sama Bajo’s small-scale fishers and land-dwellers are unique and paradox. These ties embedded in the everyday economic and social life of Sama Bajo as an Indonesian iconic maritime tribe. This article will identify key actors between Sama Bajo and varied land-dwellers. We presume that the actors support cooperation and enhance the social resilience of Sama Bajo communities in marine preserve social context. The research was conducted from May until June 2019 in three Sama Bajo villages and land-dwellers islands in Wakatobi Regency. A social mapping, using UCINET 6.0, is offered to seek key actors in the different local social context of land-dwellers. Research findings found that key actors related to trading networks, social identity, and gender dimension. Later, the actors’ social relation has contributed to the social resilience amongst three different Sama Bajo communities in Wakatobi Marine National Park.
Addition of lemuru fish oil in the diet on the fat retention and fatty acid profile of silver barb (Rasbora argyrotaenia)
Authors: Marini A , Mubarak A S , Mukti A T
Published: February 2020

One important factor in cultivation is feed. The addition of fish oil to feed affects the activity of the protein and cell synthesis so that it affects the growth rate, feed conversion ratio, and protein and fat contents of the fish body. This study aimed to determine the effect of the addition of lemuru fish oil in feed on fat retention and fatty acid profiles of silver barb (Rasbora argyrotaenia) fish. This study was used as an experimental method with a completely randomized design consisting of five treatments and four replications for each treatment. The lemuru fish oil with different concentrations, namely 0, 2, 4, 6, and 8% were added to commercial feed of silver barb. The parameters of fat retention and fatty acid profile were observed. Data were analyzed statistically using a variant of analysis followed by Duncan’s multiple range test. The results showed that the addition of lemuru fish oil in the fish feed for 40 days of fish rearing had a significant effect (p<0.05) on fat retention and increased fatty acid profile of silver barb. The 2% treatment has the best of fat retention (53.36%). A fat concentration of 9% in the diet increase unsaturated fatty acids content of silver barb (66.32%).
Addition of Lemuru Fish Oil to Protein Retention and Feed Utilization Efficiency of silver barb Rasbora argyrotaenia
Authors: Ayunda R , Sulmartiwi L , Mubarak A S
Published: February 2020

Silver barb Rasbora argyrotaenia is one of the local freshwater fish in indonesia that has advantage of being a source of protein and also resistant to disease. This study aims to determine the effect of adding lemuru fish oil to feeds on protein retention and the feed utilization efficiency of silver barb. This study used an experimental method with a complete randomized design consisting of five treatments and four replications. The treatment given is commercial food which is added with lemuru fish oil with different concentrations. In this study it can be seen that the treatments tested were: P0 (0%), P1 (2%), P2 (4%), P3 (6%), P4 (8%). The parameters observed in this study were protein retention and feed utilization efficiency of silver barb. Data analysis using Variant Analysis (ANOVA) and continued with Duncan’s Multiple Distance Test. The results showed that the addition of lemuru fish oil in feed had a significant effect (P<0,05) on protein retention and feed utilization efficiency of silver barb. The highest protein retention in treatment P2 (4%) of 16.95% and the highest efficiency of feed utilization was also found in the treatment of P2 (4%) of 57.93%.
Study on mangrove canopy cover in Lembeh Island, North Sulawesi
Authors: Rumengan A P , Paruntu C P , Paransa D , Rumengan S H
Published: February 2020

The purpose of this study is to monitor the cover of mangrove forests on Lembeh Island, North Sulawesi, to support various protections. The method used to determine mangrove health uses the value of Normalized Different Vegetation Index (NDVI). NDVI value of mangrove forest cover in 2016 and 2019 using Landsat image 8. Mangrove health in Lembeh Island Seen more in 2016 and 2019. Percentage of mangrove forest cover based on Minister of Environment Decree No. 201 of 2004 concerning standard criteria and guidelines for determining mangrove health. The rate obtained based on the dense category increased from 65% in 2016 to 67% in 2019, while mangrove in the group spares decreased from 13% percent in 2016 to 10% in 2019.
The immune responses of Oreochromis niloticus under different form of Bacillus supplementation
Authors: Yuniarti A , Arifin N B , Muawiyah N , Fakhri M , Hariati A M
Published: February 2020

Probiotic is considered an effective means for disease prevention in the aquaculture system. The most of probiotic species used in aquaculture were Bacillus in the form of vegetative cells. Therefore, this study evaluates the immune responses of Oreochromis niloticus under the Bacillus supplementation in the form of vegetative cells and spore. The addition of vegetative cells and spore Bacillus was given in the fish diet for 49 days. Several non-specific immune responses were evaluated afterward. Treatment without Bacillus supplementation in the diet was used as a control. The results showed that Bacillus supplementation in the form of spore and vegetative increased the non-specific immune response compared to those of control. Some parameters of the immune response, such as total leukocytes, haematocrit value, and respiratory burst, were affected by the form of Bacillus supplementation. Total haematocrit (31.67%), total leukocytes (8,2x104 cells.mm-3), Lymphocyte (81.33%) and respiratory burst (0.09 nm) of Tilapia with spore Bacillus supplementation were noted as the highest value. On the other hand, the phagocytosis activity of Tilapia was found statistically similar to vegetative or spore form of Bacillus supplementation. The factor that affected those results was the higher viability of the Bacillus spore in the fish diet. This study indicated that Bacillus supplementation in the form of spore gave the best improvement on the Tilapia non-specific immune response and could maintain the health status of the fish.
Growth rate and survivorship of Acropora sp. fragments that transplanted on the artificial substrate made from fly ash and bottom ash
Authors: Khasanah R I , Herawati E Y , Hariati A M , Mahmudi M , Sartimbul A , Wiadnya D G R , Asrial E , Yudatomo  , Nabil E
Published: February 2020

The purpose of this research is to analyze the success the transplantation method using kerbstone media from blocks made of coal waste (fly ash and bottom ash) from Thermal Power Station (PLTU). The research was done from March to August 2018 in the waters of Paiton, Probolinggo, East Java, Indonesia. The method applied in this research a 7-block design with “H” formation for every module. The coral fragments of Acropora formosa, Acropora pulchra, Acropora intermedia, and Acropora gomezi was 10 cm in average size with 30 repetitions for each size. The average growth rate showed different values during five months of research since the transplantation was done. Acropora formosa had the highest growth rate of 1.958 cm/month. Acropora intermedia 1.730 cm/month, Acropora pulchra 0.958 cm/month, and 0.756 cm/month for Acropora gomezi. By using Kruskal Wallis test for data analysing, obtained a survival rate of over 80% for all types, with Acropora formosa as the highest (92.3%) and Acropora gomezi as the lowest (82.61%). Therefore, kerbstone is considered feasible as a growth media for the coral reef as an effort to support CTI (Coral Triangle Initiative) program in overcoming global warming as an innovation.
The effect of using the initial weight of seedlings by the floating method on the percentage of daily growth of seaweed eucheuma cottonii.
Authors: Isroni W , Bahri A S , Amin A A
Published: February 2020

Eucheuma cottonii is widespread in coastal areas. The content of seaweed in the form of agar, carrageenan, and alginate causes seaweed to have an important meaning in industry. The problem currently faced is the lack of production of seaweed for industrial materials, caused by the lack of growth rate. The community’s understanding is that the more weight the seedlings plant, the more yields will be obtained and the faster to harvest. But in reality, the less weight the seeds planted, the faster the rate of growth, so that the seeds needed are not so many and produce a lot of harvest. The purpose of this study was to determine the effect of different initial seed weights on the growth rate of E. cottonii seaweed. This research is an experimental study, using a Completely Randomized Trial Design consisting of one factor and six replications. While the treatment used is the initial weight of different seedlings, ( 25 gr, 50 gr, 75 gr, and 100 gr). This research was conducted in the waters of Lamawalang District, Larantuka City, East Flores Regency. Data analysis technique used one-way ANOVA (one factor) with the Least Significant Difference Test level of 5%.
Provision of bacteria from shrimp pond sediment towards N/P ratio, plankton abundance, and total bacteria in the culture media of white shrimp (Litopenaeus vannamei)
Authors: Satyantini W H , Salsabila M , Nindarwi D D , Sahidu A M , Mukti A T
Published: February 2020

This study aims to determine the effect of the administration of bacteria isolated from shrimp pond sedimentBacillus mycoides, B. subtilis, andPseudomonas diminutatowards plankton abundance, N/P ratio, and total bacteria in the culture media of white shrimp (Litopenaeus vannamei). This experimental research using a Completely Randomized Design (CRD) consisting of four treatments based on the difference in the bacterial density, namely K(0 cell/mL), B1 (106cells/mL), B2 (107cells/mL), and B3 (108cells/mL), with four replications each treatment and the shrimps were carried out for 28 days. The parameters were observed are plankton abundance, N/P ratio, and total bacteria. This research showed that probiotics could increase plankton abundance, N/P ratio, and total bacteria. The results of this research indicate that the different density of bacteria from shrimp pond sediment significantly different (p<0,05) towards plankton abundance and total bacteria in the culture media of white shrimp, while the administration of bacteria from shrimp pond sediment could increase the N/P ratio. The B3 gave the best results on plankton abundance, N/P ratio, and total bacteria of 13,566.88 cells/mm3, 7.22, and 2.93 x 109cells/mL respectively.
Spawning potential ratio (SPR) of Sulphur Goatfish (Upeneus sulphureus): biological basis for demersal fishery management in Java Sea
Authors: Prayitno M A , Setiawan H , Jatmiko I , Rahman M A , Wiadnya D G R
Published: February 2020

Goatfish is one of the most dominant demersal-fish species currently caught by local fishermen in North-Coast of East Java and Madura using modified Danish-Seine. This research aimed to investigate species diversity goatfish and spawning potential ratio (SPR) of most representative species, U. suphureus. Identification was completed through morphometric and meristic investigation. A total of 2,550 fish were sub-sampled from catches for length-frequency analysis, length at first maturity (Lm), length-weight relationship and SPR. The results showed that there were five species within catches, being: U. sulphureus, U. moluccensis, U. tragula, U. sundaicus, and P. heptacanthus. Length-weight followed cubic formula with Wt = 0.0159 * Lt3.124 (p< 0.05; r2 = 0.92). Female length-at-first mature was reached at 11.67 cm Fork-Length, with total female biomass in the sample 6.5 kg. Total biomass was 93.1 kg, hence the SPR was < 7%. The minimum value of SPR while maintaining stock at good parent stock was >20%. From these results, it was very clear that demersal fishery in North-Coast of East Java, represented by U. sulphureus is unsustainable. Management measure, either size or effort limitation should be implemented, otherwise, the stock will soon collapse.
Concentration of fermented tilapia feces suspension by decomposer bacteria as a feed to induce moina macrocopa sexual reproduction
Authors: Zuhro A F , Mubarak A S , Masithah E D
Published: February 2020

Sexual induction of M. macrocopa can be induced by setting a density of feed concentration. To optimize the production of ephippia, sexual females must be given sufficient quality feed. Fermentation of tilapia fish from the results of preliminary studies showed a significant increase in protein concentration but fat concentration decreased. This research to determine the best concentration of fermented tilapia feces feed to produce ephippia M. macrocopa. This research is experimental by using a completely randomized design (CRD). This research consisted of 4 treatments of feed concentration is fermented feces suspension concentration of 33,30 mg/L (P1), 37,00 mg/L (P2) and 40,70 mg/L (P3) and control using rice bran suspension feed concentration of 37,00 mg/L(P0), with each using 5 replications. Induction sexual offspring is maintained at a density of 1000 ind/L for 6 days. During cultivation is calculated survival rate, M. macrocopa ephippia production, and some water quality parameters as support. Data were analyzed using ANOVA and Duncan test. This research showed that M. macrocopa cultivation using several concentrations of suspension feed of fermented tilapia feces at concentrations of 40.70 mg/L can induce sexual females and produce ephippia and showed the highest survival rate (1186 ± 26,45 grains/L) and 88,13%.
Antigen Candidates for Atherosclerosis Vaccine Development
Authors: Putri D K S C , Al-Farabi M J , Suryawan I G R
Published: February 2020

Atherosclerosis is a major cardiovascular issue in the world. Atherosclerosis has long been theorized to be originated from foam cells that build up due to the chronic inflammatory process of cholesterol deposition in the walls of blood vessels and the uptake of oxidized LDL (OxLDL) and phosphorylcholine. Until now, the treatment for atherosclerosis has been focusing on drugs that inhibit the progression of atherosclerotic plaques. Recent findings show that proper regulation of immune cells, both adaptive and innate, has been shown to have a relationship with the prevention of atherosclerotic lesions. The future of vaccination as prevention for atherosclerosis holds great potential, considering studies are abundant in proving the atheroprotective effects of the immunization with several types of antigens. In this literature review, we will discuss the types of antigens which hold promising potentials for atherosclerosis vaccine development and its future challenges.
Late Eyelid Reconstruction of Necrotizing Fasciitis
Authors: Muthie F A , Sutjipto
Published: February 2020

Necrotizing fasciitis (NF) is a devastating severe infection. NF involving periorbital tissue is rarely found. We reported a 42-year-old woman with eyelid edema surrounded by necrotic tissue and pus production for about a week. LRINEC score was 6. Widespread local excision and debridement were urgently performed. Reconstruction performed two weeks after the onset. The rotational flap is taken from her forehead to prompt good skin remodeling and vascularization. A part of the flap slipped underneath the temporal skin. The flap was perfectly grown, and the eyelid function recovered. NF is a rare severe infection and rarely involves the periorbital area. Early recognition is critical. LRINEC score could be used, and urgent debridement is needed. Late skin flap could be a choice to cover the debrided skin and give a good aesthetic yet functional eyelid.
Importance of Basal Soluble ST2 and Global Longitudinal Strain 2D-Speckle Tracking Echocardiography to Detect Left Ventricle Remodeling in Post-Myocardial Infarction Patients
Authors: Hasibuan F S , Aminuddin M , Utomo B , Pratama I S
Published: February 2020

Left ventricle (LV) remodeling often occurs after acute myocardial infarction (AMI), and early detection and prompt treatment on this condition may improve prognosis of the patients. Basal soluble ST2 and Global Longitudinal Strain (GLS) 2D-speckle tracking echocardiography can detect early changes before LV remodeling occurs. This is an analytic observational study using one group pre-test and post-test design for correlation. Forty-five patients of AMI during August until December 2015 from ICCU and cardiology ward were analyzed and followed up for 12 weeks. There were 45 subjects with AMI, 38 (84.4%) patients with STEMI and seven (15.6%) patients with NSTEMI. There were significant differences of left ventricle end diastolic volume (LVEDV) baseline from 76±29.69ml/m2 to 98.11±36.84ml/m2 after 12 week follow-up (p=0,003). There was significant correlation between high baseline soluble ST2 with and low GLS baseline with LV remodeling (p=0.015). High baseline soluble ST2 and low GLS value can predict LV remodeling in patients with AMI.
Left Atrial Volume Index (LAVI) as an Indicator of Severity and Pulmonary Hypertension in Mitral Stenosis
Authors: Rahman G M , Subagjo A
Published: February 2020

Left Atrial (LA) pressure and the trans-mitral pressure gradient chronically increases in Mitral Stenosis (MS), which results in LA enlargement and Pulmonary Hypertension (PH). We investigate the Left Atrial Volume Index (LAVI) as an indicator of severity and PH in MS. Fifty-nine subjects with MS (27 men and 32 women) mean ages 41.3±9.51 y.o were included in this study. We examined the correlation of LAVI and parameters of MS severity, and PH (Planimetry, MVA-PHT, MVA-VTI, MV-PHT, MV meanPG, TRmaxPG, and EstPASP). Most of the subjects had severe MS (77%), mild PH (40%) and AF (80%). LAVI value markedly increased in this study (Mean: 83.79 ± 38.32 ml/m2). In subjects with severe MS, LAVI was significantly higher than that in moderate MS (91.88±38.41 vs 54.83±19.28, p<0.05). LAVI was significantly correlated with Planimetry, MVA-PHT, MVA-VTI, MV-PHT, MV MeanPG, and EstPASP (-0.40; -0.42; -0.29; 0.42; 0.27 and 0.29, p<0.05, respectively). LAVI cut-off value as an indicator for severe MS was 59.72 ml/m2, with the sensitivity and specificity was 75.3% and 66%, respectively. LAVI was correlated well with severity and PH parameters in MS. LAVI also can be used as an indicator for severe MS.
Supine Percutaneous Nephrolitholapaxy Profile in Jombang General Hospital from 2017 until 2018
Authors: Purba J H , Surahmad F , Renaldo J
Published: February 2020

Supine Percutaneous Nephrolitholapaxy (sPNL) is a safe, minimally invasive procedure with high success rates and low morbidity for simple or complex kidney stones. This study aims to describe patient profile who underwent Supine Percutaneous Nephrolitholapaxy (sPNL), stone complexity, average length of stay, average time of procedure, stone free rate, the relationship between stone complexity with amount of puncture needed, need of re-nephroscopy, incidence of postoperative complications, and relationship between time of procedure with the incidence of postoperative complication. Analytical retrospective study was performed from February 2017 until May 2018 in Jombang General Hospital. No significant differences were found between stone complexity and number of punctures, need of re-nephroscopy, and post-operative complications. Prolonged procedure related with the incidence of postoperative complications.
Cluster of differentiation 36 (CD 36), ENac, and AQP 2 effects on heart
Authors: Sunariani J
Published: February 2020

Nowadays obesity, defined as an imbalance between food intake and exercising, has become a health concern worldwide. Obesity triggers many diseases, one of them being diabetes melitus (DM) type 2. Atherosclerosis is known to be linked with CD 36 and it can be identified as platelet integral membrane glycoprotein IV. CD 36 is known as thrombospondin-1 (TSP-1) which is found on gustatory cells, extracellular matrix (ECMs) and platelete a granules, therefore it has strong relationship with atherosclerosis. CD 36 can bind to oxLDL and induces prothrombotic state. Inspite of that, ENaC and AQP 2 also have a strong effect on heart, especially their role are often related to the heart failure (HF) because sodium and water are thought to be mediated through activation of arginine vasopression (AV), neurohormonal activity, the renal-angiotensin-aldosteron system (RAAS), and atrial natriuretic peptide that maintains cardiac output and blood pressure. This article is focused on the relationship of CD 36, ENaC, and AQP 2 effects’ on heart, including the molecular mechanism. This understanding needed to treat and to cure the heart diseases and prolong the sufferers life expectancy.
Systolic Function is Related to the Quality of Life in Chronic Heart Failure Patients
Authors: Shonafi K A , Wicaksono R B , Gunadi R I , Herdyanto R , Andrianto A
Published: February 2020

As a chronic disease, heart failure may have a significant impact on a patient’s quality of life. There are conflicting study results regarding the relationship between systolic function and quality of life in patients with chronic heart failure. This study identifies the relationship between systolic function and quality of life in patients with chronic heart failure. This study was conducted on 34 consecutive Chronic Heart Failure (CHF) patients in Cardiovascular Department, Sosodoro Djatikoesoemo General Hospital. Left Ventricle Ejection Fraction (LVEF) was obtained from echocardiography, and quality of life was assessed using Minnesota Living with Heart Failure Questionnaire (MLHFQ). A low quality of life was defined as MLHFQ score ≥ 45. The correlation between LVEF and physical, emotional dimension, and overall score from MLHFQ shows significant results (p > 0.05). The correlation between LVEF and physical dimension and between LVEF and an overall score of MLHFQ shows strong negative degree (r = -0.727). The correlation between LVEF with both physical and emotional dimension shows negative strong degree (r = -0.678, the latter r = -0.547). There is a significant correlation between systolic function and physical, emotional, and overall quality of life in chronic heart failure patients.