bluuebunny/crossref_metadata_2025 huggingface dataset:
Association of MMP-2 (–1306 C/T) Gene Polymorphism with Predisposition to Optic Neuritis and Optic Neuritis Together with Multiple Sclerosis
Authors: Liutkevičienė Rasa , Vilkevičiūtė Alvita , Banevičus Mantas , Miežytė Raminta , Kriaučiūnienė Loresa
Published: May 2018

Background and objective: Optic neuritis (ON) is characterized by painful, usually monocular vision loss with decreased visual acuity and defects of the visual field and color vision. The etiology and pathophysiology of ON is not completely clear. It is thought that a matrix metalloproteinase 2 (MMP-2) gene plays an essential role in this autoimmune inflammatory disease. The aim of this study was to determine the relationship between the MMP-2 (-1306 C/T) rs243865 gene polymorphism and ON, and that of ON with multiple sclerosis. Materials and methods: Patients with ON/ON and multiple sclerosis and a control group of healthy individuals were enrolled in this study. The genotyping test of the MMP-2 (-1306 C/T) was carried out using a real-time polymerase chain reaction (PCR) method. Results: Analysis revealed that T allele at the MMP-2 (-1306 C/T) was less frequent in the ON group compared to the control group (14.5% vs. 23.3%, p = 0.031), and was associated with decreased likelihood of ON development (OR = 0.566; 95% CI: 0.333-0.962; p = 0.036). No significant associations were revealed while comparing the subgroups of ON patients with and without multiple sclerosis. Conclusion: The MMP-2 (-1306 C/T) gene polymorphism was found to be associated with ON development.
Laser Acupuncture and Heart Rate Variability—Scientific Considerations
Authors: Litscher Gerhard
Published: May 2018

n/a
A Comparative Study on the Effect of Welding Parameters of Austenitic Stainless Steels Using Artificial Neural Network and Taguchi Approaches with ANOVA Analysis
Authors: Kurt Halil Ibrahim , Oduncuoglu Murat , Yilmaz Necip Fazil , Ergul Engin , Asmatulu Ramazan
Published: May 2018

In order to investigate the structure of welds, austenitic stainless steel (SS) studs with a diameter of 6 mm were welded to austenitic SS plates with a thickness of 5 mm using an arc stud welding (ASW) method. The effects of the welding current, welding time, and tip volume of the stud on the microstructure and ultimate tensile strength (UTS) of the welded samples were investigated in detail. The formation of δ-ferrites was detected in the weld zone because of the higher heat generated during the welding process. Higher welding current and time adversely affected the stud and significantly reduced the UTS of the samples. The UTS of the joints was also estimated using artificial neural network (ANN) and Taguchi approaches. The mathematical formulations for these two approaches were given in explicit form. Experimental results showed that the neural network results are more consistent with experimental results than those of the Taguchi method. Overall, it can be concluded that in order to achieve good welding joints and high strength values, ASW parameters should be investigated properly to determine the optimum conditions for each metal.
Distribution of Selected Trace Elements in the Bayer Process
Authors: Vind Johannes , Alexandri Alexandra , Vassiliadou Vicky , Panias Dimitrios
Published: May 2018

The aim of this work was to achieve an understanding of the distribution of selected bauxite trace elements (gallium (Ga), vanadium (V), arsenic (As), chromium (Cr), rare earth elements (REEs), scandium (Sc)) in the Bayer process. The assessment was designed as a case study in an alumina plant in operation to provide an overview of the trace elements behaviour in an actual industrial setup. A combination of analytical techniques was used, mainly inductively coupled plasma mass spectrometry and optical emission spectroscopy as well as instrumental neutron activation analysis. It was found that Ga, V and As as well as, to a minor extent, Cr are principally accumulated in Bayer process liquors. In addition, Ga is also fractionated to alumina at the end of the Bayer processing cycle. The rest of these elements pass to bauxite residue. REEs and Sc have the tendency to remain practically unaffected in the solid phases of the Bayer process and, therefore, at least 98% of their mass is transferred to bauxite residue. The interest in such a study originates from the fact that many of these trace constituents of bauxite ore could potentially become valuable by-products of the Bayer process; therefore, the understanding of their behaviour needs to be expanded. In fact, Ga and V are already by-products of the Bayer process, but their distribution patterns have not been provided in the existing open literature.
Tuning Interface to Improve Corrosion Resistance of Electroless Ni-P Coating on AZ31B Alloy
Authors: Ma Beilei , Wang Ruihong
Published: May 2018

Interface is crucial to enable desirable service performances of coatings on substrates. In this paper, Ni-P coatings were prepared on AZ31B alloy by using electroless plating, with the coating/substrate interface being tuned to improve the corrosion resistance. The interface tuning involved a phosphate treatment prior to the electroless plating, which created a uniform surface of the Mg substrate and finally led to Ni-P plating coatings with enhanced density during the electroless plating. Electrochemical testing was performed to compare the corrosion properties between the Ni-P coatings with and without phosphate treatment. The experimental results evidently showed that the introduction of phosphate treatment, especially after an annealing treatment, greatly improved the corrosion resistance. The underlying mechanisms, revealed by microstructural examinations, were that the phosphate treatment reduced the substrate surface roughness and likely promoted a high and uniform nucleation intensity of the Ni-P coating. Corrosion processes of the unannealed and annealed Ni-P coatings with an interfacial phosphate layer were further clarified for comparison.
Study of a Bimetallic Interfacial Bonding Process Based on Ultrasonic Quantitative Evaluation
Authors: Zhang Qingdong , Li Shuo , Liu Jiyang , Wang Yanan , Zhang Boyang , Zhang Li-Yuan
Published: May 2018

The interfacial bonding process and mechanical properties of AISI (American Iron and Steel Institute) stainless steel/Q235A carbon steel were investigated by deformation bonding, heat preservation, and tensile tests. The results reveal that the deformation temperature has a beneficial effect on the bonding rate, and that the critical temperature for the large-area bonding of stainless steel and carbon steel is approximately 700 °C. However, owing to the short contact time, only a shallow diffusion of approximately 2–3 µm can be achieved. A slight decrease in the interfacial bonding rate after heat preservation is correlated to the difference between the thermal expansion coefficients of the two materials, but the thickness of the diffusion layer shows a significant increase. It was found that the strength of the composites is mostly related to the bonding rate and the strength of the relatively soft material, which can be determined using an explicit equation. Moreover, with the increase in the interfacial bonding rate, the crack source changes from the junction of the unbonded and bonded areas of the interface to the interior of the carbon steel. The failure mode evolves from cleavage brittle fracture to ductile–brittle mixed-mode fracture, and then to ductile fracture.
Influence of the Velocity and the Number of Polishing Passages on the Roughness of Electrolytic Plasma Polished Pipe Inner Surfaces
Authors: Cornelsen Matthias , Deutsch Carolin , Seitz Hermann
Published: May 2018

Electrolytic plasma polishing (EPP) is an emerging technology for polishing, cleaning, deburring and smoothing of free-formed metal surfaces. The electrolytic plasma polishing of outer metal surfaces is state-of-the-art, whereas the polishing of pipe inner surfaces has only recently been reported by the authors. A prototype system and first experimental results were presented. It was found in the previous study that the average surface roughness

Sa

reaches a range from 0.065 µm to 0.090 µm. The current study systematically investigates the influence of the velocity v as well as the number n of polishing passages on the average surface roughness

Sa

. The polishing of the pipe inner surface and weld seam are considered separately. The results show that the average roughness

Sa

is mainly dependent on the effective polishing time

t

e
p
t

of the polishing process. The average surface roughness

Sa

of the pipe inner surface can reach a range from 0.030 µm to 0.034 µm starting from an initial surface roughness

S

a
0

of 0.719 µm, whereas the average surface roughness

Sa

of the weld seam can reach a range from 0.088 µm to 0.096 µm starting from an initial surface roughness

Sa
0

of 0.282 µm. These ranges are achieved after an effective polishing time of approximately 25 s for both the inner pipe surface and the weld seam.
Study on Micro-Crack Induced Precision Severing of Quartz Glass Chips
Authors: Zhang Long , Xie Jin , Guo Aodian
Published: May 2018

It is difficult to cut hard and brittle quartz glass chips. Hence, a method involving micro-crack-induced severing along a non-crack microgroove-apex by controlling the loading rate is proposed. The objective is to realize the rapid and precision severing of the hardest quartz glass in chip materials. Firstly, micro-grinding was employed to machine smooth microgrooves of 398–565 μ m in depth; then the severing force was modelled by the microgroove shape and size; finally, the severing performance of a 4-mm thick substrate was investigated experimentally. It is shown that the crack propagation occurred at the same time from the microgroove-apex and the loading point during 0.5 ms in micro-crack-induced severing. The severing efficiency is dominated by the severing time rather than the crack propagation time. When the loading rate is less than 20–60 mm/min, the dynamic severing is transferred to static severing. With increasing microgroove-apex radius, the severing force decreases to the critical severing force of about 160–180 N in the static severing, but it increases to the critical severing force in the dynamic severing. The static severing force and time are about two times and about nine times larger than the dynamic ones, respectively, but the static severing form error of 16.3 μ m/mm and surface roughness of 19.7 nm are less. It is confirmed that the ideal static severing forces are identical to the experimental results. As a result, the static severing is controllable for the accurate and smooth separation of quartz glass chips in 4 s and less.
Structure Simulation and Host–Guest Interaction of Histidine-Intercalated Hydrotalcite–Montmorillonite Complex
Authors: Wang Chen-Xi , Pu Min , Zhang Pei-Huan , Gao Yang , Yang Zuo-Yin , Lei Ming
Published: May 2018

The structures of histidine intercalated hydrotalcite–montmorillonite complex (His–LDHs–MMT) were studied using the DMol3 code, GGA/PW91 function, and DND basis set of the density functional theory (DFT). The geometries of His–LDHs–MMT were optimized, and their electronic properties were calculated. The results showed that the structure of the complex can be seen as that the quaternary ammonium group of histidine was adsorbed on the oxygen of MMT lamella, and its oxygen on the carboxylic acid anion was combined with the hydrogen atoms of the LDHs lamella. It was determined that the interaction mainly consisted in hydrogen bonding and electrostatic force. The average binding energies per histidine of His–LDHs and His–MMT were about −65.89 and −78.44 kcal/mol, respectively. The density of states of the complexes showed that the 2p orbitals of oxygen were dominant, and the 1s orbit of hydrogen near the Fermi level indicate the formation of hydrogen bonds in the complex. The charge density data displayed the density field of histidine carboxylic acid anion overlapped with that of hydrotalcite layer, indicating that a strong hydrogen bond interaction existed between histidine and hydrotalcite layer. The analysis of the electrostatic potential of complex indicated that the electrostatic interaction between histidine and MMT is obviously stronger than that of LDHs. The simulated XRD spectra showed the special diffraction peaks of LDHs and MMT layer in the complex.
Looking Like Gold: Chlorite and Talc Transformation in the Golden Slip Ware Production (Swat Valley, North-Western Pakistan)
Authors: Maritan Lara , Piovesan Rebecca , Dalconi Maria Chiara , Rius Jordi , Crespi Anna , Vallcorba Oriol , Casas Lluís , Vidale Massimo , Olivieri Luca Maria
Published: May 2018

The archaeometric study of the “golden slip” ware (second century BCE—fourth century CE) at the site of Barikot (Swat, north-western Pakistan) aimed to define its manufacturing technology and provenance of the raw materials used. For this reason, a multianalytical approach consisting of the microscopic, microstructural and mineralogical analysis of both the golden slip and the ceramic paste was adopted. The slip was found to be composed by platy minerals, microchemically identified as talc and chlorite; their intimate association indicated clearly that they derived from a chlorite-talc schist. This rock is geologically available near the site in the “green stones” lenses within the Mingora ophiolites outcropping in the Swat valley. Due to the use of this stone also for the production of stone tools, it cannot be excluded that the chlorite-talc schist used for the golden slip can be derived from manufacturing residues of the Gandharan sculptures. In order to constrain the firing production technology, laboratory replicas were produced using a locally collected clay and coating them with ground chlorite-talc schist. On the basis of the mineralogical association observed in both the slip and the ceramic paste and the thermodynamic stability of the pristine mineral phases, the golden slip pottery underwent firing under oxidising conditions in the temperature interval between 800 °C and 850 °C. The golden and shining looks of the slip were here interpreted as the result of the combined light reflectance of the platy structure of the talc-based coating and the uniform, bright red colour of the oxidized ceramic background.
Mineral Pigments in Archaeology: Their Analysis and the Range of Available Materials
Authors: Siddall Ruth
Published: May 2018

Naturally occurring minerals or their synthetic analogues have been important as pigments used in artistic and cosmetic contexts in global antiquity. The analysis and identification of mineral pigments, though routine to the petrologist or mineralogist, also requires specialist knowledge of the archaeological contexts and available technologies and trade. This paper attempts to present an analytical approach to the study of mineral pigments in archaeology and also introduces the range of mineral pigments encountered in works of art and painted objects on archaeological sites and in museums. It attempts to cover the range of mineral and synthetic inorganic pigments used in global cultures from to the early Medieval period.
Fabrication of Refractive Index Tunable Coating with Moisture-Resistant Function for High-Power Laser Systems Based on Homogeneous Embedding of Surface-Modified Nanoparticles
Authors: Yang Wei , Lei Xiangyang , Hui Haohao , Zhang Qinghua , Deng Xueran
Published: May 2018

Moisture-resistant silicone coatings were prepared on the surface of potassium dihydrogen phosphate (KDP) crystal by means of spin-coating, in which hydrophobic-modified SiO2 nanoparticles were embedded in a certain proportion. The refractive index of such coating can be tuned arbitrarily in the range of 1.21–1.44, which endows the KDP optical component with excellent transmission capability as well as the moisture proof effect. A dual-layer anti-reflective coating system was obtained by covering this silicone coating with a porous SiO2 coating which is specially treated to enhance the moisture resistance. Transmittance of such a dual-layer coating system could reach 99.60% and 99.62% at 1064 nm and 532 nm, respectively, by precisely matching the refractive index of both layers. Furthermore, the long-term stability of this coating system has been verified at high humidity ambient of 80% RH for 27 weeks.
Xanthine Oxidase Inhibitory Activity and Thermostability of Cinnamaldehyde-Chemotype Leaf Oil of Cinnamomum osmophloeum Microencapsulated with β-Cyclodextrin
Authors: Huang Chi-Ya , Yeh Ting-Feng , Hsu Fu-Lan , Lin Chun-Ya , Chang Shang-Tzen , Chang Hui-Ting
Published: May 2018

The xanthine oxidase inhibitory activity and thermostability of Cinnamomum osmophloeum leaf oil microencapsulated with β-cyclodextrin were evaluated in this study. The yield of leaf oil microcapsules was 86.3% using the optimal reaction conditions at the leaf oil to β-cyclodextrin ratio of 15:85 and ethanol to water ratio ranging from 1:3 to 1:5. Based on the FTIR analysis, the characteristic absorption bands of major constituent, trans-cinnamaldehyde, were confirmed in the spectra of leaf oil microcapsules. According to the dry-heat aging test, β-cyclodextrin was thermostable under the high temperature conditions, and it was beneficial to reduce the emission of C. osmophloeum leaf oil. Leaf oil microcapsules exhibited high xanthine oxidase inhibitory activity, with an IC50 value of 83.3 µg/mL. It is concluded that the lifetime of C. osmophloeum leaf oil can be effectively improved by microencapsulation, and leaf oil microcapsules possess superior xanthine oxidase inhibitory activity.
C19-Norditerpenoid Alkaloids from Aconitum szechenyianum
Authors: Song Bei , Jin Bingliang , Li Yuze , Wang Fei , Yang Yifu , Cui Yuwen , Song Xiaomei , Yue Zhenggang , Liu Jianli
Published: May 2018

Three new C19-norditerpenoid alkaloids (1–3), along with two known C19-norditerpenoid alkaloids (4,5), have been isolated from Aconitum szechenyianum. Based on extensive spectroscopic techniques (1D, 2D-NMR, IR, and MS) and chemical methods, their structures were established as szechenyianine D (1), szechenyianine E (2), szechenyianine F (3), 8-O-methyl-14-benzoylaconine (4), and spicatine A (5). The immunosuppressive effects of compounds 1–5 were studied using a ConA-induced or LPS-induced splenocyte proliferation model. In vitro tests showed that Compounds 2, 4, and 5 suppressed ConA-induced or LPS-induced splenocyte proliferation in a concentration-dependent manner. The CC50/IC50 values of 2, 4, and 5 suggested that these compounds were potential immunosuppressive agents for the treatment of autoimmune diseases characterized by arthritis, such as rheumatoid arthritis.
Efficient Approach for the Extraction and Identification of Red Pigment from Zanthoxylum bungeanum Maxim and Its Antioxidant Activity
Authors: Chen Xi , Wei Zhiqiang , Zhu Lei , Yuan Xing , Wei Daneng , Peng Wei , Wu Chunjie
Published: May 2018

Red pigment (RP) was extracted from the peels of Zanthoxylum bungeanum Maxim (PZB) by ultrasonic-assisted extraction (UAE) in this work. Box–Behnken design–response surface methodology (BBD-RSM) was employed to research the efficiency of the RP extraction. Based on the optimization of RSM, results showed that the optimal extraction conditions were as follows: liquid–solid ratio of 31 mL/g, extraction time of 41 min, and extraction temperature of 27 °C, and under these conditions, the actual absorbance value was 0.615 ± 0.13%, highly agreeing with the predicted value by the model. Furthermore, ultra-performance liquid chromatography–mass spectrometry (UPLC-MS) was used to separate and analyze RP. The compounds of RP were mainly flavonoids, and there were five compounds detected for the first time in PZB. In addition, RP showed significant antioxidant activities in vitro, which could be developed for anti-aging candidate drugs and functional foods. In conclusion, ultrasound-assisted extraction with BBD-RSM and chromatographic separation technology with UPLC-MS are efficient strategies for the isolation and identification of RP from PZB.
Determination of Biogenic Amines in Seawater Using Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection
Authors: Gubartallah Elbaleeq A. , Makahleh Ahmad , Quirino Joselito P. , Saad Bahruddin
Published: May 2018

A rapid and green analytical method based on capillary electrophoresis with capacitively coupled contactless conductivity detection (C4D) for the determination of eight environmental pollutants, the biogenic amines (putrescine, cadaverine, spermidine, spermine, tyramine, 2-phenylamine, histamine and tryptamine), is described. The separation was achieved under normal polarity mode at 24 °C and 25 kV with a hydrodynamic injection (50 mbar for 5 s) and using a bare fused-silica capillary (95 cm length × 50 µm i.d.) (detection length of 10.5 cm from the outlet end of the capillary). The optimized background electrolyte consisted of 400 mM malic acid. C4D parameters were set at a fixed amplitude (50 V) and frequency (600 kHz). Under the optimum conditions, the method exhibited good linearity over the range of 1.0–100 µg mL−1 (R2 ≥ 0.981). The limits of detection based on signal to noise (S/N) ratios of 3 and 10 were ≤0.029 µg mL−1. The method was used for the determination of seawater samples that were spiked with biogenic amines. Good recoveries (77–93%) were found.
A 3D-QSAR Study on the Antitrypanosomal and Cytotoxic Activities of Steroid Alkaloids by Comparative Molecular Field Analysis
Authors: Nnadi Charles Okeke , Althaus Julia Barbara , Nwodo Ngozi Justina , Schmidt Thomas Jürgen
Published: May 2018

As part of our research for new leads against human African trypanosomiasis (HAT), we report on a 3D-QSAR study for antitrypanosomal activity and cytotoxicity of aminosteroid-type alkaloids recently isolated from the African medicinal plant Holarrhena africana A. DC. (Apocynaceae), some of which are strong trypanocides against Trypanosoma brucei rhodesiense (Tbr), with low toxicity against mammalian cells. Fully optimized 3D molecular models of seventeen congeneric Holarrhena alkaloids were subjected to a comparative molecular field analysis (CoMFA). CoMFA models were obtained for both, the anti-Tbr and cytotoxic activity data. Model performance was assessed in terms of statistical characteristics (R2, Q2, and P2 for partial least squares (PLS) regression, internal cross-validation (leave-one-out), and external predictions (test set), respectively, as well as the corresponding standard deviation error in prediction (SDEP) and F-values). With R2 = 0.99, Q2 = 0.83 and P2 = 0.79 for anti-Tbr activity and R2 = 0.94, Q2 = 0.64, P2 = 0.59 for cytotoxicity against L6 rat skeletal myoblasts, both models were of good internal and external predictive power. The regression coefficients of the models representing the most prominent steric and electrostatic effects on anti-Tbr and for L6 cytotoxic activity were translated into contour maps and analyzed visually, allowing suggestions for possible modification of the aminosteroids to further increase the antitrypanosomal potency and selectivity. Very interestingly, the 3D-QSAR model established with the Holarrhena alkaloids also applied to the antitrypanosomal activity of two aminocycloartane-type compounds recently isolated by our group from Buxus sempervirens L. (Buxaceae), which indicates that these structurally similar natural products share a common structure–activity relationship (SAR) and, possibly, mechanism of action with the Holarrhena steroids. This 3D-QSAR study has thus resulted in plausible structural explanations of the antitrypanosomal activity and selectivity of aminosteroid- and aminocycloartane-type alkaloids as an interesting new class of trypanocides and may represent a starting point for lead optimization.
Investigation of the Antifungal Activity and Mode of Action of Thymus vulgaris, Citrus limonum, Pelargonium graveolens, Cinnamomum cassia, Ocimum basilicum, and Eugenia caryophyllus Essential Oils
Authors: Gucwa Katarzyna , Milewski Sławomir , Dymerski Tomasz , Szweda Piotr
Published: May 2018

The antimicrobial activity of plant oils and extracts has been recognized for many years. In this study the activity of Thymus vulgaris, Citrus limonum, Pelargonium graveolens, Cinnamomum cassia, Ocimum basilicum, and Eugenia caryophyllus essential oils (EOs) distributed by Pollena Aroma (Nowy Dwór Mazowiecki, Poland) was investigated against a group of 183 clinical isolates of C. albicans and 76 isolates of C. glabrata. All of the oils exhibited both fungistatic and fungicidal activity toward C. albicans and C. glabrata isolates. The highest activity was observed for cinnamon oil, with MIC (Minimum Inhibitory Concentration) values in the range 0.002–0.125% (v/v). The MIC values of the rest of the oils were in the range 0.005% (or less) to 2.5% (v/v). In most cases MFC (Minimum Fungicidal Concentration) values were equal to MIC or twice as high. Additionally, we examined the mode of action of selected EOs. The effect on cell wall components could not be clearly proved. Three of the tested EOs (thyme, lemon, and clove) affected cell membranes. At the same time, thyme, cinnamon, and clove oil influenced potassium ion efflux, which was not seen in the case of lemon oil. All of the tested oils demonstrated the ability to inhibit the transition of yeast to mycelium form, but the effect was the lowest in the case of cinnamon oil.
Soy Protein Isolate-Phosphatidylcholine Nanoemulsions Prepared Using High-Pressure Homogenization
Authors: Li Yang , Wu Chang-Ling , Liu Jun , Zhu Ying , Zhang Xiao-Yuan , Jiang Lian-Zhou , Qi Bao-Kun , Zhang Xiao-Nan , Wang Zhong-Jiang , Teng Fei
Published: May 2018

The nanoemulsions of soy protein isolate-phosphatidylcholine (SPI-PC) with different emulsion conditions were studied. Homogenization pressure and homogenization cycle times were varied, along with SPI and PC concentration. Evaluations included turbidity, particle size, ζ-potential, particle distribution index, and turbiscan stability index (TSI). The nanoemulsions had the best stability when SPI was at 1.5%, PC was at 0.22%, the homogenization pressure was 100 MPa and homogenization was performed 4 times. The average particle size of the SPI-PC nanoemulsions was 217 nm, the TSI was 3.02 and the emulsification yield was 93.4% of nanoemulsions.
Characterization of Nanoparticle Batch-To-Batch Variability
Authors: Mülhopt Sonja , Diabaté Silvia , Dilger Marco , Adelhelm Christel , Anderlohr Christopher , Bergfeldt Thomas , Gómez de la Torre Johan , Jiang Yunhong , Valsami-Jones Eugenia , Langevin Dominique , Lynch Iseult , Mahon Eugene , Nelissen Inge , Piella Jordi , Puntes Victor , Ray Sikha , Schneider Reinhard , Wilkins Terry , Weiss Carsten , Paur Hanns-Rudolf
Published: May 2018

A central challenge for the safe design of nanomaterials (NMs) is the inherent variability of NM properties, both as produced and as they interact with and evolve in, their surroundings. This has led to uncertainty in the literature regarding whether the biological and toxicological effects reported for NMs are related to specific NM properties themselves, or rather to the presence of impurities or physical effects such as agglomeration of particles. Thus, there is a strong need for systematic evaluation of the synthesis and processing parameters that lead to potential variability of different NM batches and the reproducible production of commonly utilized NMs. The work described here represents over three years of effort across 14 European laboratories to assess the reproducibility of nanoparticle properties produced by the same and modified synthesis routes for four of the OECD priority NMs (silica dioxide, zinc oxide, cerium dioxide and titanium dioxide) as well as amine-modified polystyrene NMs, which are frequently employed as positive controls for nanotoxicity studies. For 46 different batches of the selected NMs, all physicochemical descriptors as prioritized by the OECD have been fully characterized. The study represents the most complete assessment of NMs batch-to-batch variability performed to date and provides numerous important insights into the potential sources of variability of NMs and how these might be reduced.
Efficacy of a Gluten-Free Diet in the Gilles de la Tourette Syndrome: A Pilot Study
Authors: Rodrigo Luis , Álvarez Nuria , Fernández-Bustillo Enrique , Salas-Puig Javier , Huerta Marcos , Hernández-Lahoz Carlos
Published: May 2018

The Gilles de la Tourette syndrome (GTS) and Non-Coeliac Gluten Sensitivity (NCGS) may be associated. We analyse the efficacy of a gluten-free diet (GFD) in 29 patients with GTS (23 children; six adults) in a prospective pilot study. All of them followed a GFD for one year. The Yale Global Tics Severity Scale (YGTSS), the Yale-Brown Obsessive-Compulsive Scale—Self Report (Y-BOCS) or the Children’s Yale-Brown Obsessive-Compulsive Scale—Self Report (CY-BOCS), and the Cavanna’s Quality of Life Questionnaire applied to GTS (GTS-QOL) were compared before and after the GFD; 74% of children and 50% of adults were males, not significant (NS). At the beginning of the study, 69% of children and 100% of adults had associated obsessive-compulsive disorder (OCD) (NS). At baseline, the YGTSS scores were 55.0 ± 17.5 (children) and 55.8 ± 19.8 (adults) (NS), the Y-BOCS/CY-BOCS scores were 15.3, (standard deviation (SD) = 12.3) (children) and 26.8 (9.2) (adults) (p = 0.043), and the GTS-QOL scores were 42.8 ± 18.5 (children) and 64 ± 7.9 (adults) (p = 0.000). NCGS was frequent in both groups, with headaches reported by 47.0% of children and 83.6% of adults (p = 0.001). After one year on a GFD there was a marked reduction in measures of tics (YGTSS) (p = 0.001), and the intensity and frequency of OCD (Y-BOCS/CY-BOCS) (p = 0.001), along with improved generic quality of life (p = 0.001) in children and adults. In conclusion, a GFD maintained for one year in GTS patients led to a marked reduction in tics and OCD both in children and adults.
The Association between Sleep Duration and 25-Hydroxyvitamin D Concentration with Obesity in an Elderly Korean Population: A Cross-Sectional Study
Authors: Doo Miae
Published: May 2018

Studies have recently reported an association between sleep duration and obesity in some individuals. Vitamin D deficiency is common in elderly populations and is also associated with obesity. In this study, the combined interaction effect of vitamin D levels and sleep duration on obesity-related variables was analyzed in 3757 individuals from an elderly Korean population using the Korean National Health and Nutrition Examination Survey. Significant differences were observed in the interaction effect of the vitamin D levels and sleep duration on obesity-related variables, including BMI (p = 0.004) and the risk of obesity (p < 0.001). Using vitamin D sufficient status and proper sleep duration as a reference, subjects with sufficient vitamin D did not differ in their risk of obesity regardless of their sleep duration. However, the risk of obesity tended to increase with short sleep duration rather than proper sleep duration among subjects who were vitamin D insufficient odds ratio (OR) (95% CI) = 1.293 (1.10–1.657) for proper sleep duration vs. 1.374 (1.066–1.770) for short sleep duration). Only the participants in the vitamin D deficient population who consumed less protein showed an increasing trend in the risk of obesity according to the sleep duration (OR (95% CI) = 1.645 (1.155–2.344) for proper sleep duration and 1.668 (1.156–2.406) for short sleep duration).
Microbiome Responses to an Uncontrolled Short-Term Diet Intervention in the Frame of the Citizen Science Project
Authors: Klimenko Natalia S. , Tyakht Alexander V. , Popenko Anna S. , Vasiliev Anatoly S. , Altukhov Ilya A. , Ischenko Dmitry S. , Shashkova Tatiana I. , Efimova Daria A. , Nikogosov Dmitri A. , Osipenko Dmitrii A. , Musienko Sergey V. , Selezneva Kseniya S. , Baranova Ancha , Kurilshikov Alexander M. , Toshchakov Stepan M. , Korzhenkov Aleksei A. , Samarov Nazar I. , Shevchenko Margarita A. , Tepliuk Alina V. , Alexeev Dmitry G.
Published: May 2018

Personalized nutrition is of increasing interest to individuals actively monitoring their health. The relations between the duration of diet intervention and the effects on gut microbiota have yet to be elucidated. Here we examined the associations of short-term dietary changes, long-term dietary habits and lifestyle with gut microbiota. Stool samples from 248 citizen-science volunteers were collected before and after a self-reported 2-week personalized diet intervention, then analyzed using 16S rRNA sequencing. Considerable correlations between long-term dietary habits and gut community structure were detected. A higher intake of vegetables and fruits was associated with increased levels of butyrate-producing Clostridiales and higher community richness. A paired comparison of the metagenomes before and after the 2-week intervention showed that even a brief, uncontrolled intervention produced profound changes in community structure: resulting in decreased levels of Bacteroidaceae, Porphyromonadaceae and Rikenellaceae families and decreased alpha-diversity coupled with an increase of Methanobrevibacter, Bifidobacterium, Clostridium and butyrate-producing Lachnospiraceae- as well as the prevalence of a permatype (a bootstrapping-based variation of enterotype) associated with a higher diversity of diet. The response of microbiota to the intervention was dependent on the initial microbiota state. These findings pave the way for the development of an individualized diet.
Impact of Dietary Macronutrient Intake during Early and Late Gestation on Offspring Body Composition at Birth, 1, 3, and 5 Years of Age
Authors: Brei Christina , Stecher Lynne , Meyer Dorothy Marie , Young Veronika , Much Daniela , Brunner Stefanie , Hauner Hans
Published: May 2018

Dietary intake during pregnancy as a possible modifiable risk factor for childhood obesity is poorly explored. In a prospective observational study, two multivariable regression models were therefore used to associate maternal diet at 15 and 32 weeks’ gestation with offsprings’ body composition and fat distribution at birth, 1, 3, and 5 years. Mean energy intake was 2157 ± 375 kcal (n = 186) in early and 2208 ± 460 kcal (n = 167) in late gestation. The partition model showed mostly no significant associations between maternal diet in early pregnancy and offspring body composition. In late pregnancy, higher fat intake was negatively associated with clinical outcomes at birth, 1, and 5 years. Protein intake was negatively associated with BMI z score (zBMI) at 3 and 5 years. A 10 g increase in fiber was associated with an increase of 3.50 mm2 abdominal subcutaneous fat at 1, 172.49 g fat mass at 3, and 0.23 zBMI at 5 years. Results were largely comparable in the substitution model. An incremental increase in fat and protein at the expense of carbohydrates in late but not early pregnancy may be associated with lower fat mass up to 5 years. Findings require confirmation by additional prospective studies.
Protective Effect of Glucosinolates Hydrolytic Products in Neurodegenerative Diseases (NDDs)
Authors: Jaafaru Mohammed Sani , Abd Karim Nurul Ashikin , Enas Mohamad Eliaser , Rollin Patrick , Mazzon Emanuela , Abdull Razis Ahmad Faizal
Published: May 2018

Crucifer vegetables, Brassicaceae and other species of the order Brassicales, e.g., Moringaceae that are commonly consumed as spice and food, have been reported to have potential benefits for the treatment and prevention of several health disorders. Though epidemiologically inconclusive, investigations have shown that consumption of those vegetables may result in reducing and preventing the risks associated with neurodegenerative disease development and may also exert other biological protections in humans. The neuroprotective effects of these vegetables have been ascribed to their secondary metabolites, glucosinolates (GLs), and their related hydrolytic products, isothiocyanates (ITCs) that are largely investigated for their various medicinal effects. Extensive pre-clinical studies have revealed more than a few molecular mechanisms of action elucidating multiple biological effects of GLs hydrolytic products. This review summarizes the most significant and up-to-date in vitro and in vivo neuroprotective actions of sulforaphane (SFN), moringin (MG), phenethyl isothiocyanate (PEITC), 6-(methylsulfinyl) hexyl isothiocyanate (6-MSITC) and erucin (ER) in neurodegenerative diseases.
Changes of Blood Pressure and Hemodynamic Parameters after Oral Magnesium Supplementation in Patients with Essential Hypertension—An Intervention Study
Authors: Banjanin Nikolina , Belojevic Goran
Published: May 2018

The objective of this study was to examine the changes of blood pressure and hemodynamic parameters after oral magnesium supplementation in patients with essential hypertension. The single-arm non-blinded intervention study comprised 48 patients (19 men; 29 women) whose antihypertensive therapy was not changed for at least one month. The participants were asked to consume (daily at home) 300 mg of oral magnesium-oxide supplementation product for one month and to have their blood pressure and hemodynamic parameters (thoracic fluid content, stroke volume, stroke index, cardiac output, cardiac index, acceleration index, left cardiac work index and systemic vascular resistance index, heart rate) measured in the hospital before and after the intervention. Measurements were performed with impedance cardiography. After magnesium supplementation, systolic and diastolic pressures were significantly decreased (mean ± standard deviation (SD)/mmHg/from 139.7 ± 15.0 to 130.8 ± 13.4 and from 88.0 ± 10.4 to 82.2 ± 9.0, respectively; both p < 0.001). The two significant hemodynamic changes were the decrease of systemic vascular resistance index (dyn s m2/cm5) and left cardiac work index (kg m/m²)/mean ± SD from 2319.3 ± 753.3 to 2083.0 ± 526.9 and from 4.8 ± 1.4 to 4.4 ± 0.9, respectively; both p < 0.05). The observed hemodynamic changes may explain lowering blood pressure after magnesium supplementation.
Dental Students’ Knowledge and Attitudes towards Antibiotic Prescribing Guidelines in Riyadh, Saudi Arabia
Authors: AboAlSamh Abdulrahman , Alhussain Abdulmalik , Alanazi Nawaf , Alahmari Rakan , Shaheen Naila , Adlan Abdallah
Published: May 2018

Background: The use of antibiotics prophylactically and therapeutically in dentistry has become common practice. Inappropriate prescription may lead to adverse side effects and bacterial resistance. During clinical training, dental students in Saudi Arabia are authorized to prescribe antibiotics. Aim: To evaluate dental students’ knowledge and attitudes regarding antibiotic prescription in Riyadh, Saudi Arabia. Methods: A cross-sectional study based on a validated questionnaire consisting of 34 questions focusing on antibiotic indications in dentistry, antibiotic regimens, and knowledge regarding resistance was distributed amongst dental students in five leading dental colleges in Riyadh. Results: A large proportion of students (71.7%) were familiar with the concept of antibiotic resistance. When comparing junior and senior dental students’ knowledge with regards to indications of antibiotic use in commonly encountered conditions, it was found that there was no significant difference in antibiotic prescription frequency between these groups. Most dental students choose to prescribe amoxicillin as their first-choice of antibiotic (88.4%), and most also chose to use it for a duration of 3–5 days (69.2%). Conclusions: This study concludes that dental students may prescribe antibiotics inappropriately to manage various conditions when not indicated. This may indicate a defect in education of students with regards to current antibiotic guidelines.
The Coordination and Luminescence of the Eu(III) Complexes with the Polymers (PMMA, PVP)
Authors: Zhao Weican , Shao Haifeng , Yu Guang , Hou Yanjun , Wang Shuhong
Published: May 2018

The rare earth complexes and the polymers can be made into composite nanofibers through electrospinning. The fluorescence intensity of these fiber composites is much higher than that of the rare earth complexes. By changing the mixed proportion of polymethyl methacrylate (PMMA) and complexes, nanofiber materials were prepared. Then, by measuring their fluorescence intensity, it is found that the carbonyl bond of PMMA may have coordinated with the rare earth ions and enhanced the luminescence intensity of them. Then, a series of experiments were designed to study their coordination and luminescence mechanism. The coordination mechanism of the polymers with carbonyl groups and the rare earth complexes was explained by Eu(TFT)3(TPPO), Eu(TFT)3(TPPO)2, Eu(PFP)3(TPPO), Eu(PFP)3(TPPO)2, and polyvinyl pyrrolidone (PVP) dissolved in chloroform solution, where TFT means 2-(2,2,2-trifluoroethyl)-1-tetralone, PFP means 2-(2,2,3,3,3-Pentafluoropro-panoyl)-3,4-dihydronaphthalen-1(2H)-one and TPPO means phosphine oxide. The coordination of PVP and the rare earth complexes in solution was studied, and it was found that the fluorine atoms of the ligand had a significant impact on the aggregation-induced effect of the composites. The electron transitioned in the polymers and the complexes were enhanced greatly by the coordination. The colors of emission light could be adjusted by the coordination of the polymers and the rare earth complexes.
Biocomposites Based on Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) (PHBHV) and Miscanthus giganteus Fibers with Improved Fiber/Matrix Interface
Authors: Rodi Erica Gea , Langlois Valérie , Renard Estelle , Sansalone Vittorio , Lemaire Thibault
Published: May 2018

In this paper, green biocomposites based on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV) and Miscanthus giganteus fibers (MIS) were prepared in the presence of dicumyl peroxide (DCP) via reactive extrusion. The objective of this study was to optimize the interfacial adhesion between the reinforcement and the matrix, improving the mechanical properties of the final material. To this aim, two fibers mass fractions (5 and 20 wt %) and two different fiber sizes obtained by two opening mesh sieves (1 mm and 45 μm) were investigated. The impregnation of fibers with DCP before processing was carried out in order to promote the PHBHV grafting onto MIS fibers during the process, favoring, in this way, the interfacial adhesion between fibers and matrix, instead of the crosslinking of the matrix. All composites were realized by extrusion and injection molding processing and then characterized by tensile tests, FTIR-ATR, SEM, DSC and XRD. According to the improved adhesion of fibers to matrix due to DCP, we carried out an implementation of models involving that can predict the effective mechanical properties of the biocomposites. Three phases were taken into account here: fibers, gel (crosslinked matrix), and matrix fractions. Due to the complexity of the system (matrix–crosslinked matrix–fibers) and to the lack of knowledge about all the phenomena occurring during the reactive extrusion, a mathematical approach was considered in order to obtain information about the modulus of the crosslinked matrix and its fraction in the composites. This study aims to estimate these last values, and to clarify the effect caused by the presence of vegetal fibers in a composite in which different reactions are promoted by DCP.
Phase Morphology of NR/SBR Blends: Effect of Curing Temperature and Curing Time
Authors: Klat Darja , Karimi-Varzaneh Hossein Ali , Lacayo-Pineda Jorge
Published: May 2018

The morphology of natural rubber/styrene–butadiene rubber blends (NR/SBR) was characterized by atomic force microscopy (AFM), with regard to curing temperature and curing time. The changes in blend morphology were directly visualized by AFM which confirmed the results of indirect experiments like differential scanning calorimetry (DSC). Comparing the phase morphologies at different curing temperatures indicated that the domain size of SBR increases with temperature at lower curing temperatures, but domain growing stops at the latest scorch time. This effect is explained by longer scorch times at low curing temperatures which facilitate phase separation, while the short scorch times at higher temperatures meant that the coalescence of SBR phases was hindered by cross-linking between polymer chains.
A General Framework for Portfolio Theory—Part I: Theory and Various Models
Authors: Maier-Paape Stanislaus , Zhu Qiji Jim
Published: May 2018

Utility and risk are two often competing measurements on the investment success. We show that efficient trade-off between these two measurements for investment portfolios happens, in general, on a convex curve in the two-dimensional space of utility and risk. This is a rather general pattern. The modern portfolio theory of Markowitz (1959) and the capital market pricing model Sharpe (1964), are special cases of our general framework when the risk measure is taken to be the standard deviation and the utility function is the identity mapping. Using our general framework, we also recover and extend the results in Rockafellar et al. (2006), which were already an extension of the capital market pricing model to allow for the use of more general deviation measures. This generalized capital asset pricing model also applies to e.g., when an approximation of the maximum drawdown is considered as a risk measure. Furthermore, the consideration of a general utility function allows for going beyond the “additive” performance measure to a “multiplicative” one of cumulative returns by using the log utility. As a result, the growth optimal portfolio theory Lintner (1965) and the leverage space portfolio theory Vince (2009) can also be understood and enhanced under our general framework. Thus, this general framework allows a unification of several important existing portfolio theories and goes far beyond. For simplicity of presentation, we phrase all for a finite underlying probability space and a one period market model, but generalizations to more complex structures are straightforward.
Privacy-Preserving Authentication Using a Double Pseudonym for Internet of Vehicles
Authors: Cui Jie , Xu Wenyu , Zhong Hong , Zhang Jing , Xu Yan , Liu Lu
Published: May 2018

The Internet of Vehicles (IoV) plays an important role in smart transportation to reduce the drivers’s risk of having an accident and help them manage small emergencies. Therefore, security and privacy issues of the message in the tamper proof device (TPD) broadcasted to other vehicles and roadside units (RSUs) have become an important research subject in the field of smart transportation. Many authentication schemes are proposed to tackle the challenges above and most of them are heavy in computation and communication. In this paper, we propose a novel authentication scheme that utilizes the double pseudonym method to hide the real identity of vehicles and adopts the dynamic update technology to periodically update the information (such as member secret, authentication key, internal pseudo-identity) stored in the tamper-proof device to prevent the side-channel attack. Because of not using bilinear pairing, our scheme yields a better performance in terms of computation overhead and communication overhead, and is more suitable to be applied in the Internet of Vehicles.