bluuebunny/crossref_metadata_2025 huggingface dataset:
Two Single Nucleotide Polymorphisms in the Purinergic Receptor P2X7 Gene Are Associated with Disease Severity in Multiple Sclerosis
Authors: Guerini Franca Rosa , Agliardi Cristina , Bolognesi Elisabetta , Zanzottera Milena , Caputo Domenico , Pasanisi Maria Barbara , Rovaris Marco , Clerici Mario
Published: December 2022

Multiple sclerosis (MS) is a chronic autoimmune inflammatory disease of the central nervous system (CNS) that leads to progressive physical disability. Recent evidence has suggested that P2X7 receptor (P2X7R)-mediated purinergic signalling pathways play a role in MS-associated neuroinflammation, possibly contributing to disease pathogenesis. To evaluate possible associations between P2X7R polymorphisms and MS disease severity, we performed an association study of five non-synonymous SNPs coding variants of the P2X7R gene: rs1718119 Ala348Thr, rs2230911 Thr357Ser, rs2230912 Gln460Arg, rs3751143 Glu496Ala, and rs28360457 Arg307Gln, modulating P2X7R expression in 128 MS patients (relapsing remitting MS, RRMS: n = 94; secondary progressive, SPMS: n = 34). All patients were genotyped, and multiple sclerosis severity score (MSSS) was evaluated in every case; 189 healthy subjects were enrolled as well as controls. Results showed that P2X7R rs1718119(A) 348Thr and rs22390912(G) 464Arg, two SNPs of minor allele frequency (MAF) known to confer gain of function to the P2X7R protein, were associated with significantly higher MSSS in RRMS patients alone (SMRR (p < 0.001, p = 0.01, respectively)). Interestingly, two whole haplotypes resulted in having significant association with MSSS in these same patients. Thus: (1) the P2X7R-4 “ACGAG” haplotype, characterized by the co-presence of the rs1718119-rs2230912 AG MAF alleles, was associated with higher MSSS (Beta: 1.11 p = 0.04), and (2) the P2X7R-1 “GCAAG” complementary haplotype, which contains the rs1718119 and rs2230912 GA wild-type alleles, was more frequently carried by patients with lower MSSS and less severe disease (Beta: −1.54 p < 0.001). Although being preliminary and needing confirmation in an ampler cohort, these results suggest that 348Thr and 464Arg variants have a role as modulators of disease severity in RRMS patients.
Structural and Immunologic Properties of the Major Soybean Allergen Gly m 4 Causing Anaphylaxis
Authors: Finkina Ekaterina I. , Bogdanov Ivan V. , Ziganshin Rustam H. , Strokach Nikita N. , Melnikova Daria N. , Toropygin Ilia Y. , Matveevskaya Natalia S. , Ovchinnikova Tatiana V.
Published: December 2022

Gly m 4 is the major soybean allergen, causing birch pollen cross allergic reactions. In some cases, Gly m 4-mediated anaphylaxis takes place, but the causative factors are still unknown. Here, we studied the structural and immunologic properties of Gly m 4 to shed light on this phenomenon. We showed that Gly m 4 retained its structure and IgE-binding capacity after heating. Gly m 4 was cleaved slowly under nonoptimal gastric conditions mimicking duodenal digestion, and IgE from the sera of allergic patients interacted with the intact allergen rather than with its proteolytic fragments. Similar peptide clusters of Bet v 1 and Gly m 4 were formed during allergen endolysosomal degradation in vitro, but their sequence identity was insignificant. Animal polyclonal anti-Gly m 4 and anti-Bet v 1 IgG weakly cross-reacted with Bet v 1 and Gly m 4, respectively. Thus, we supposed that not only conserved epitopes elicited cross-reactivity with Bet v 1, but also variable epitopes were present in the Gly m 4 structure. Our data suggests that consumption of moderately processed soybean-based drinks may lead to the neutralizing of gastric pH as a result of which intact Gly m 4 can reach the human intestine and cause IgE-mediated system allergic reactions.
Pharmacomicrobiomics in Pediatric Oncology: The Complex Interplay between Commonly Used Drugs and Gut Microbiome
Authors: Leardini Davide , Venturelli Francesco , Baccelli Francesco , Cerasi Sara , Muratore Edoardo , Brigidi Patrizia , Pession Andrea , Prete Arcangelo , Masetti Riccardo
Published: December 2022

The gut microbiome (GM) has emerged in the last few years as a main character in several diseases. In pediatric oncological patients, GM has a role in promoting the disease, modulating the effectiveness of therapies, and determining the clinical outcomes. The therapeutic course for most pediatric cancer influences the GM due to dietary modifications and several administrated drugs, including chemotherapies, antibiotics and immunosuppressants. Interestingly, increasing evidence is uncovering a role of the GM on drug pharmacokinetics and pharmacodynamics, defining a bidirectional relationship. Indeed, the pediatric setting presents some contrasts with respect to the adult, since the GM undergoes a constant multifactorial evolution during childhood following external stimuli (such as diet modification during weaning). In this review, we aim to summarize the available evidence of pharmacomicrobiomics in pediatric oncology.
Gilthead Seabream Liver Integrative Proteomics and Metabolomics Analysis Reveals Regulation by Different Prosurvival Pathways in the Metabolic Adaptation to Stress
Authors: Raposo de Magalhães Cláudia , Farinha Ana Paula , Blackburn Gavin , Whitfield Phillip D. , Carrilho Raquel , Schrama Denise , Cerqueira Marco , Rodrigues Pedro M.
Published: December 2022

The study of the molecular mechanisms of stress appraisal on farmed fish is paramount to ensuring a sustainable aquaculture. Stress exposure can either culminate in the organism’s adaptation or aggravate into a metabolic shutdown, characterized by irreversible cellular damage and deleterious effects on fish performance, welfare, and survival. Multiomics can improve our understanding of the complex stressed phenotype in fish and the molecular mediators that regulate the underlying processes of the molecular stress response. We profiled the stress proteome and metabolome of Sparus aurata responding to different challenges common to aquaculture production, characterizing the disturbed pathways in the fish liver, i.e., the central organ in mounting the stress response. Label-free shotgun proteomics and untargeted metabolomics analyses identified 1738 proteins and 120 metabolites, separately. Mass spectrometry data have been made fully accessible via ProteomeXchange, with the identifier PXD036392, and via MetaboLights, with the identifier MTBLS5940. Integrative multivariate statistical analysis, performed with data integration analysis for biomarker discovery using latent components (DIABLO), depicted the 10 most-relevant features. Functional analysis of these selected features revealed an intricate network of regulatory components, modulating different signaling pathways related to cellular stress, e.g., the mTORC1 pathway, the unfolded protein response, endocytosis, and autophagy to different extents according to the stress nature. These results shed light on the dynamics and extent of this species’ metabolic reprogramming under chronic stress, supporting future studies on stress markers’ discovery and fish welfare research.
Identification of the Role of TGR5 in the Regulation of Leydig Cell Homeostasis
Authors: Holota Hélène , De Haze Angélique , Martinot Emmanuelle , Monrose Melusine , Saru Jean-Paul , Caira Françoise , Beaudoin Claude , Volle David H.
Published: December 2022

Understanding the regulation of the testicular endocrine function leading to testosterone production is a major objective as the alteration of endocrine function is associated with the development of many diseases such as infertility. In the last decades, it has been demonstrated that several endogenous molecules regulate the steroidogenic pathway. Among them, bile acids have recently emerged as local regulators of testicular physiology and particularly endocrine function. Bile acids act through the nuclear receptor FXRα (Farnesoid-X-receptor alpha; NR1H4) and the G-protein-coupled bile acid receptor (GPBAR-1; TGR5). While FXRα has been demonstrated to regulate testosterone synthesis within Leydig cells, no data are available regarding TGR5. Here, we investigated the potential role of TGR5 within Leydig cells using cell culture approaches combined with pharmacological exposure to the TGR5 agonist INT-777. The data show that activation of TGR5 results in a decrease in testosterone levels. TGR5 acts through the PKA pathway to regulate steroidogenesis. In addition, our data show that TGR5 activation leads to an increase in cholesterol ester levels. This suggests that altered lipid homeostasis may be a mechanism explaining the TGR5-induced decrease in testosterone levels. In conclusion, the present work highlights the impact of the TGR5 signaling pathway on testosterone production and reinforces the links between bile acid signaling pathways and the testicular endocrine function. The testicular bile acid pathways need to be further explored to increase our knowledge of pathologies associated with impaired testicular endocrine function, such as fertility disorders.
Impact of IBD-Associated Dysbiosis on Bacterial Quorum Sensing Mediated by Acyl-Homoserine Lactone in Human Gut Microbiota
Authors: Grellier Nathan , Suzuki Marcelino T. , Brot Loic , Rodrigues Alice M. S. , Humbert Lydie , Escoubeyrou Karine , Rainteau Dominique , Grill Jean-Pierre , Lami Raphaël , Seksik Philippe
Published: December 2022

Intestinal dysbiosis is a key feature in the pathogenesis of inflammatory bowel disease (IBD). Acyl-homoserine lactones (AHL) are bacterial quorum-sensing metabolites that may play a role in the changes in host cells-gut microbiota interaction observed during IBD. The objective of our study was to investigate the presence and expression of AHL synthases and receptor genes in the human gut ecosystem during IBD. We used an in silico approach, applied to the Inflammatory Bowel Disease Multi’omics Database comprising bacterial metagenomic and metatranscriptomic data from stools of patients with Crohn’s disease (CD) (n = 50), ulcerative colitis (UC) (n = 27) and non-IBD controls (n = 26). No known putative AHL synthase gene was identified; however, several putative luxR receptors were observed. Regarding the expression of these receptor genes, the luxR gene from Bacteroides dorei was under-expressed in IBD patients (p = 0.02) compared to non-IBD patients, especially in CD patients (p = 0.02). In the dysbiosis situation, one luxR receptor gene from Bacteroides fragilis appeared to be over-expressed (p = 0.04) compared to that of non-dysbiotic patients. Targeting LuxR receptors of bacterial quorum sensing might represent a new approach to modulate the gut microbiota in IBD.
A Cross-Sectional Study on the Occurrence of the Intestinal Protist, Dientamoeba fragilis, in the Gut-Healthy Volunteers and Their Animals
Authors: Jirků Milan , Kašparová Andrea , Lhotská Zuzana , Oborník Miroslav , Brožová Kristýna , Petrželková Klára J. , Samaš Peter , Kadlecová Oldřiška , Stensvold Christen Rune , Jirků Kateřina
Published: December 2022

Dientamoeba fragilis is a cosmopolitan intestinal protist colonizing the human gut with varying prevalence depending on the cohort studied and the diagnostic methods used. Its role in human health remains unclear mainly due to the very sporadic number of cross-sectional studies in gut-healthy populations. The main objective of this study was to expand knowledge of the epidemiology of D. fragilis in gut-healthy humans and their animals. A total of 296 stool samples from humans and 135 samples from 18 animal species were analyzed. Using qPCR, a prevalence of 24% was found in humans in contrast to conventional PCR (7%). In humans, several factors were found to influence the prevalence of D. fragilis. A more frequent occurrence of D. fragilis was associated with living in a village, traveling outside Europe and contact with farm animals. In addition, co-infection with Blastocystis spp. was observed in nearly half of the colonized humans. In animals, D. fragilis was detected in 13% of samples from eight species using qPCR. Our molecular phylogenies demonstrate a more frequent occurrence of Genotype 1 in gut-healthy humans and also revealed a likely a new protist species/lineage in rabbits related to D. fragilis and other related organisms.
miR−122−5p Regulates Renal Fibrosis In Vivo
Authors: Kaneko Shohei , Yanai Katsunori , Ishii Hiroki , Aomatsu Akinori , Hirai Keiji , Ookawara Susumu , Ishibashi Kenichi , Morishita Yoshiyuki
Published: December 2022

The role of exogenous microRNAs (miRNAs) in renal fibrosis is poorly understood. Here, the effect of exogenous miRNAs on renal fibrosis was investigated using a renal fibrosis mouse model generated by unilateral ureteral obstruction (UUO). miRNA microarray analysis and quantitative reverse-transcription polymerase chain reaction showed that miR−122−5p was the most downregulated (0.28-fold) miRNA in the kidneys of UUO mice. The injection of an miR−122−5p mimic promoted renal fibrosis and upregulated COL1A2 and FN1, whereas an miR−122−5p inhibitor suppressed renal fibrosis and downregulated COL1A2 and FN1. The expression levels of fibrosis-related mRNAs, which were predicted targets of miR−122−5p, were evaluated. The expression level of TGFBR2, a pro-fibrotic mRNA, was upregulated by the miR−122−5p mimic, and the expression level of FOXO3, an anti−fibrotic mRNA, was upregulated by the miR−122−5p inhibitor. The protein expressions of TGFBR2 and FOXO3 were confirmed by immunohistochemistry. Additionally, the expression levels of LC3, downstream anti-fibrotic mRNAs of FOXO3, were upregulated by the miR−122−5p inhibitor. These results suggest that miR−122−5p has critical roles in renal fibrosis.
Dimethyl Fumarate Alleviates Adult Neurogenesis Disruption in Hippocampus and Olfactory Bulb and Spatial Cognitive Deficits Induced by Intracerebroventricular Streptozotocin Injection in Young and Aged Rats
Authors: Kurowska-Rucińska Ewelina , Ruciński Jan , Myślińska Dorota , Grembecka Beata , Wrona Danuta , Majkutewicz Irena
Published: December 2022

The disorder of adult neurogenesis is considered an important mechanism underlying the learning and memory impairment observed in Alzheimer’s disease (AD). The sporadic nonhereditary form of AD (sAD) affects over 95% of AD patients and is related to interactions between genetic and environmental factors. An intracerebroventricular injection of streptozotocin (STZ-ICV) is a representative and well-established method to induce sAD-like pathology. Dimethyl fumarate (DMF) has antioxidant and anti-inflammatory properties and is used for multiple sclerosis treatment. The present study determines whether a 26-day DMF therapy ameliorates the disruption of adult neurogenesis and BDNF-related neuroprotection in the hippocampus and olfactory bulb (OB) in an STZ-ICV rat model of sAD. Considering age as an important risk factor for developing AD, this study was performed using 3-month-old (the young group) and 22-month-old (the aged group) male Wistar rats. Spatial cognitive functions were evaluated with the Morris water maze task. Immunofluorescent labelling was used to assess the parameters of adult neurogenesis and BDNF-related neuroprotection in the hippocampus and OB. Our results showed that the STZ-ICV evoked spatial learning and memory impairment and disturbances in adult neurogenesis and BDNF expression in both examined brain structures. In the aged animals, the deficits were more severe. We found that the DMF treatment significantly alleviated STZ-ICV-induced behavioural and neuronal disorders in both age groups of the rats. Our findings suggest that DMF, due to its beneficial effect on the formation of new neurons and BDNF-related neuroprotection, may be considered as a promising new therapeutic agent in human sAD.
Lipid Droplet-Associated Proteins Perilipin 1 and 2: Molecular Markers of Steatosis and Microvesicular Steatotic Foci in Chronic Hepatitis C
Authors: Schelbert Selina , Schindeldecker Mario , Drebber Uta , Witzel Hagen Roland , Weinmann Arndt , Dries Volker , Schirmacher Peter , Roth Wilfried , Straub Beate Katharina
Published: December 2022

Chronic infection with hepatitis C (HCV) is a major risk factor in the development of cirrhosis and hepatocellular carcinoma. Lipid metabolism plays a major role in the replication and deposition of HCV at lipid droplets (LDs). We have demonstrated the importance of LD-associated proteins of the perilipin family in steatotic liver diseases. Using a large collection of 231 human liver biopsies with HCV, perilipins 1 and 2 have been localized to LDs of hepatocytes that correlate with the degree of steatosis and specific HCV genotypes, but not significantly with the HCV viral load. Perilipin 1- and 2-positive microvesicular steatotic foci were observed in 36% of HCV liver biopsies, and also in chronic hepatitis B, autoimmune hepatitis and mildly steatotic or normal livers, but less or none were observed in normal livers of younger patients. Microvesicular steatotic foci did not frequently overlap with glycogenotic/clear cell foci as determined by PAS stain in serial sections. Steatotic foci were detected in all liver zones with slight architectural disarrays, as demonstrated by immunohistochemical glutamine synthetase staining of zone three, but without elevated Ki67-proliferation rates. In conclusion, microvesicular steatotic foci are frequently found in chronic viral hepatitis, but the clinical significance of these foci is so far not clear.
Transcriptomic Analysis of Distal Parts of Roots Reveals Potentially Important Mechanisms Contributing to Limited Flooding Tolerance of Canola (Brassica napus) Plants
Authors: Liu Mengmeng , Zwiazek Janusz J.
Published: December 2022

Since most of the root metabolic activities as well as root elongation and the uptake of water and mineral nutrients take place in the distal parts of roots, we aimed to gain insight into the physiological and transcriptional changes induced by root hypoxia in the distal parts of roots in canola (Brassica napus) plants, which are relatively sensitive to flooding conditions. Plants were subject to three days of root hypoxia via lowering oxygen content in hydroponic medium, and various physiological and anatomical features were examined to characterize plant responses. Untargeted transcriptomic profiling approaches were also applied to investigate changes in gene expression that took place in the distal root tissues in response to hypoxia. Plants responded to three days of root hypoxia by reducing growth and gas exchange rates. These changes were accompanied by decreases in leaf water potential (Ψleaf) and root hydraulic conductivity (Lpr). Increased deposition of lignin and suberin was also observed in the root tissues of hypoxic plants. The transcriptomic data demonstrated that the effect of hypoxia on plant water relations involved downregulation of most BnPIPs in the root tissues with the exception of BnPIP1;3 and BnPIP2;7, which were upregulated. Since some members of the PIP1 subfamily of aquaporins are known to transport oxygen, the increase in BnPIP1;3 may represent an important hypoxia tolerance strategy in plants. The results also demonstrated substantial rearrangements of different signaling pathways and transcription factors (TFs), which resulted in alterations of genes involved in the regulation of Lpr, TCA (tricarboxylic acid) cycle-related enzymes, antioxidant enzymes, and cell wall modifications. An integration of these data enabled us to draft a comprehensive model of the molecular pathways involved in the responses of distal parts of roots in B. napus. The model highlights systematic transcriptomic reprogramming aimed at explaining the relative sensitivity of Brassica napus to root hypoxia.
The Stabilizing Effect of Carboxymethyl Cellulose on Foamed Concrete
Authors: Ji Yongcheng , Sun Qijun
Published: December 2022

Foam concrete is widely used for its excellent properties, such as light weight, heat insulation, fire resistance, and sound insulation. The stability of foam is the main factor that affects the mechanical performance of foam concrete. The experiments are designed from two perspectives: the foam’s stability performance and the foam concrete’s modification effect. The effects on foam volume, foam half-life, foam bleeding rate, and foam pore size were investigated based on different concentrations of foam stabilizer CMC (0%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%). A combination of macroscopic testing and microscopic analysis, a comparative study of dry density, water absorption test, mechanical property test, and pore structure analysis were conducted after using the modified foam for foam concrete. It is shown that the addition of CMC has an enhanced effect on foam stability. Foaming volume, water secretion rate, and average pore size showed a decreasing trend with the increase of CMC admixture, while the foam half-life displayed an increasing trend. In addition, adding CMC reduces the dry density and improves water absorption and compressive strength. The pore structure development of foam concrete has a noticeable improvement effect, and the optimal amount of admixture is 0.4%. Research results provide a reference for applying thickening foam stabilizer CMC in foam concrete.
Activated PyK2 and Its Associated Molecules Transduce Cellular Signaling from the Cancerous Milieu for Cancer Metastasis
Authors: Lee Dongun , Hong Jeong-Hee
Published: December 2022

PyK2 is a member of the proline-rich tyrosine kinase and focal adhesion kinase families and is ubiquitously expressed. PyK2 is mainly activated by stimuli, such as activated Src kinases and intracellular acidic pH. The mechanism of PyK2 activation in cancer cells has been addressed extensively. The up-regulation of PyK2 through overexpression and enhanced phosphorylation is a key feature of tumorigenesis and cancer migration. In this review, we summarized the cancer milieu, including acidification and cancer-associated molecules, such as chemical reagents, interactive proteins, chemokine-related molecules, calcium channels/transporters, and oxidative molecules that affect the fate of PyK2. The inhibition of PyK2 leads to a beneficial strategy to attenuate cancer cell development, including metastasis. Thus, we highlighted the effect of PyK2 on various cancer cell types and the distribution of molecules that affect PyK2 activation. In particular, we underlined the relationship between PyK2 and cancer metastasis and its potential to treat cancer cells.
Non-Alcoholic Fatty Liver Disease (NAFLD) Pathogenesis and Natural Products for Prevention and Treatment
Authors: Guo Xiangyu , Yin Xunzhe , Liu Zuojia , Wang Jin
Published: December 2022

Non-alcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver disease, affecting approximately one-quarter of the global population, and has become a world public health issue. NAFLD is a clinicopathological syndrome characterized by hepatic steatosis, excluding ethanol and other definite liver damage factors. Recent studies have shown that the development of NAFLD is associated with lipid accumulation, oxidative stress, endoplasmic reticulum stress, and lipotoxicity. A range of natural products have been reported as regulators of NAFLD in vivo and in vitro. This paper reviews the pathogenesis of NAFLD and some natural products that have been shown to have therapeutic effects on NAFLD. Our work shows that natural products can be a potential therapeutic option for NAFLD.
The Electrophoretic Deposition of Nanopowders Based on Yttrium Oxide for Bulk Ceramics Fabrication
Authors: Kalinina Elena , Ivanov Maxim
Published: December 2022

In the present work, a study was carried out to investigate the key factors that determine the uniformity, mass, thickness, and density of compacts obtained from nanopowders of solid solutions of yttrium and lanthanum oxides ((LaxY1−x)2O3) with the help of the electrophoretic deposition (EPD). Nanopowders were obtained by laser ablation of a mixture of powders of yttrium oxide and lanthanum oxide in air. The implemented mechanisms of the EPD and factors of stability of alcohol suspensions are analyzed. It has been shown that acetylacetone with a concentration of 1 mg/m2 can be used as a dispersant for stabilization of isopropanol suspensions of the nanoparticles during the EPD. It was shown that the maximum density of dry compacts with a thickness of 2.4 mm reaches 37% of theoretical when EPD is performed in vertical direction from a suspension of nanopowders with addition of acetylacetone.
Organoselenocyanates Tethered Methyl Anthranilate Hybrids with Promising Anticancer, Antimicrobial, and Antioxidant Activities
Authors: Al-Abdallah Batool , Al-Faiyz Yasair S. , Shaaban Saad
Published: December 2022

Novel methyl anthranilate-based organoselenocyanate hybrids were developed, and their structures were confirmed by the state-of-the-art spectroscopic techniques. Their antimicrobial potency was estimated against various microbial strains (e.g., Candida albicans, Escherichia coli, and Staphylococcus aureus). The S. aureus and C. albicans strains were more sensitive than E. coli toward the organoselenocyanates. Interestingly, the azoic derivatives 4 and 9, methyl ester 6, and phenoxy acetamide 15 showed promising antimicrobial activity. Moreover, the antitumor potential was estimated against liver and breast carcinomas, as well as primary fibroblasts. Interestingly, the anticancer properties were more pronounced in the HepG2 cells. The organoselenocyanates 4, 6, 9, 10, and 15 showed interesting anti-HepG2 cytotoxic patterns. Additionally, organoselenocyanates 3, 4, and 10 exhibited promising antioxidant activities in the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid and 2,2-diphenyl-1-picrylhydrazyl in vitro assays compared to ascorbic acid. These data point to promising antimicrobial, anticancer, and antioxidant activities of organoselenocyanates 6, 9, and 15 warrant further studies.
Exogenous Calcium Suppresses the Oviposition Choices of Frankliniella occidentalis (Thysanoptera: Thripidae) and Promotes the Attraction of Orius similis (Hemiptera: Anthocoridae) by Altering Volatile Blend Emissions in Kidney Bean Plants
Authors: Huang Wan-Qing , Zeng Guang , Zhi Jun-Rui , Qiu Xin-Yue , Yin Zhen-Juan
Published: December 2022

Frankliniella occidentalis is a destructive pest of horticultural plants, while Orius similis is a natural enemy of thrips. It has been demonstrated that exogenous calcium could induce plant defenses against herbivore attack. We examined whether CaCl2 supplementation altered the volatile emissions of kidney bean plants, which influence the oviposition preference of F. occidentalis. We also assessed the influence of volatile cues on O. similis. Using Y-tube olfactometer tests, we found that exogenous CaCl2 treatment inhibited the selectivity of F. occidentalis but attracted O. similis. In addition, CaCl2 treatment reduced the oviposition preference of F. occidentalis. Gas chromatography–mass spectrometry analyses revealed that CaCl2 treatment altered the number and relative abundance of the volatile compounds in kidney bean plants and that (E)-2-hexen-1-ol, 1-octen-3-ol, β-lonone, and (E,E)-2,4-hexadienal might be potential olfactory cues. Furthermore, the results of the six-arm olfactometer test indicated that 1-octen-3-ol (10−2 μL/μL), β-lonone (10−2 μL/μL), and (E,E)-2,4-hexadienal (10−3 μL/μL) repelled F. occidentalis but attracted O. similis. Overall, our results suggested that exogenous CaCl2 treatment induced defense responses in kidney bean plants, suggesting that CaCl2 supplementation may be a promising strategy to enhance the biological control of F. occidentalis.
Pulmonary Vascular Remodeling and Prognosis in Patients Evaluated for Heart Transplantation: Insights from the OCTOPUS-CHF Study
Authors: Martínez-Solano Jorge , Gutiérrez-Ibañes Enrique , Ortiz-Bautista Carlos , García-Cosío María Dolores , Sarnago-Cebada Fernando , Díaz-Molina Beatriz , Pascual Isaac , Oteo-Domínguez Juan Francisco , Gómez-Bueno Manuel , Calviño-Santos Ramón , Crespo-Leiro María G. , Gómez-Hospital Joan Antoni , Díez-López Carles , García-Lara Juan , Garrido-Bravo Iris P. , de la Fuente-Galán Luis , López-Díaz Javier , Mirabet-Pérez Sonia , Martínez-Sellés Manuel
Published: December 2022

Objective: In patients with advanced heart failure, the intravascular optical coherence tomography (OCT) of subsegmental pulmonary artery measurements is correlated with right heart catheterization parameters. Our aim was to study the prognostic value of pulmonary OCT, right heart catheterization data, and the echocardiographic estimation of pulmonary pressure in patients studied for elective heart transplants. Methods: This research is an observational, prospective, multicenter study involving 90 adults with a one-year follow-up. Results: A total of 10 patients (11.1%) died due to worsening heart failure before heart transplantation, 50 underwent a heart transplant (55.6%), and 9 died in the first year after the transplant. The patients with and without events (mortality or heart failure-induced hospitalization) had similar data regarding echocardiography, right heart catheterization, and pulmonary OCT (with a median estimated pulmonary artery systolic pressure of 42.0 mmHg, interquartile range (IQR) of 30.3–50.0 vs. 47.0 mmHg, IQR 34.6–59.5 and p = 0.79, median pulmonary vascular resistance of 2.2 Wood units, IQR 1.3–3.7 vs. 2.0 Wood units, IQR 1.4–3.2 and p = 0.99, and a median pulmonary artery wall thickness of 0.2 ± 0.5 mm vs. 0.2 ± 0.6 mm and p = 0.87). Conclusion: Pulmonary vascular remodeling (evaluated with echocardiography, right heart catheterization, and pulmonary OCT) was not associated with prognosis in a selected sample of adults evaluated for elective heart transplants. Pulmonary OCT is safe and feasible for the evaluation of these patients.
Blood Pressure Prediction Using Ensemble Rules during Isometric Sustained Weight Test
Authors: Carrazana-Escalona Ramón , Andreu-Heredia Adán , Moreno-Padilla María , Reyes del Paso Gustavo A. , Sánchez-Hechavarría Miguel E. , Muñoz-Bustos Gustavo
Published: December 2022

Background: Predicting beat-to-beat blood pressure has several clinical applications. While most machine learning models focus on accuracy, it is necessary to build models that explain the relationships of hemodynamical parameters with blood pressure without sacrificing accuracy, especially during exercise. Objective: The aim of this study is to use the RuleFit model to measure the importance, interactions, and relationships among several parameters extracted from photoplethysmography (PPG) and electrocardiography (ECG) signals during a dynamic weight-bearing test (WBT) and to assess the accuracy and interpretability of the model results. Methods: RuleFit was applied to hemodynamical ECG and PPG parameters during rest and WBT in six healthy young subjects. The WBT involves holding a 500 g weight in the left hand for 2 min. Blood pressure is taken in the opposite arm before and during exercise thereof. Results: The root mean square error of the model residuals was 4.72 and 2.68 mmHg for systolic blood pressure and diastolic blood pressure, respectively, during rest and 4.59 and 4.01 mmHg, respectively, during the WBT. Furthermore, the blood pressure measurements appeared to be nonlinear, and interaction effects were observed. Moreover, blood pressure predictions based on PPG parameters showed a strong correlation with individual characteristics and responses to exercise. Conclusion: The RuleFit model is an excellent tool to study interactions among variables for predicting blood pressure. Compared to other models, the RuleFit model showed superior performance. RuleFit can be used for predicting and interpreting relationships among predictors extracted from PPG and ECG signals.
Prevalence, Predictors and Mechanisms of Steam Pops in Ablation Index-Guided High-Power Pulmonary Vein Isolation
Authors: Shuang Tian , Kong Lingcong , Cheng Fuyu , Wang Xinhua
Published: December 2022

Despite the good cooling effect of the contact-force porous catheter, the risk of steam pops (SP) remains one of the major concerns in high-power circumferential pulmonary vein isolation (CPVI). This study aimed to investigate the prevalence, predictors and possible mechanisms of SPs in CPVI. Patients experiencing SPs in de novo high-power CPVI were 1:3 matched by non-SP patients with gender, age (±5 years) and left atrial diameter (LAD) (±5 mm) to compare the ablation parameters of SP and non-SP lesions. Catheter tip displacement (Tipdisp) was compared between “edge-of-ridge” and “PV-side-of-ridge” placement at anterior and roof segments of the left pulmonary vein (PV). SPs occurred in 11 (1.57%) of 701 patients, including 6 at the antero-superior left PV, 2 at the roof, 1 at the postero-superior left PV, 1 at the bottom left PV and 1 at the antero-superior aspect of the right PV. There was significantly shorter RF delivery duration (13.9 ± 6.3 vs. 23.3 ± 6.0 s), greater Δimpedance (17.6 ± 6.7 vs. 6.7 ± 4.1 Ω) and lower ablation index (357.7 ± 68.8 vs. 430.2 ± 30.7) in SP patients than those in non-SP patients. Δimpedance >12 Ω during ablation could predict SP occurrence. Tipdisp was greater in “PV-side-of-ridge” than that in “edge-of -ridge” placement (3.2 ± 1.6 mm vs. 2.0 ± 0.8 mm) at antero-superior and roof segments of the left PV. The prevalence of SP was 1.57% in high-power CPVI procedures, with the most common site at the antero-superior segment of the left PV. Δimpedance was a significant predictor of SP occurrence. “PV-side-of-ridge” ablation at antero-superior and roof segments of left PV might predispose to SP occurrence due to excessive tissue coverage.
Reduced Skeletal Muscle Mass Is Associated with an Increased Risk of Asthma Control and Exacerbation
Authors: Zhang Shuwen , Zhang Xin , Deng Ke , Wang Changyong , Wood Lisa G. , Wan Huajing , Liu Lei , Wang Ji , Zhang Li , Liu Ying , Cheng Gaiping , Gibson Peter G. , Oliver Brian G. , Luo Fengming , McDonald Vanessa M. , Li Weimin , Wang Gang
Published: December 2022

Background: Skeletal muscle mass (SMM) has been suggested to be associated with multiple health-related outcomes. However, the potential influence of SMM on asthma has not been largely explored. Objective: To study the association between SMM and clinical features of asthma, including asthma control and exacerbation, and to construct a model based on SMM to predict the risk of asthma exacerbation (AEx). Methods: In this prospective cohort study, we consecutively recruited patients with asthma (n = 334), classified as the SMM Normal group (n = 223), SMM Low group (n = 88), and SMM High group (n = 23). We investigated the association between SMM and clinical asthma characteristics and explored the association between SMM and asthma control and AEx within a 12-month follow-up period. Based on SMM, an exacerbation prediction model was developed, and the overall performance was externally validated in an independent cohort (n = 157). Results: Compared with the SMM Normal group, SMM Low group exhibited more airway obstruction and worse asthma control, while SMM High group had a reduced eosinophil percentage in induced sputum. Furthermore, SMM Low group was at a significantly increased risk of moderate-to-severe exacerbation compared with the SMM Normal group (relative risk adjusted 2.02 [95% confidence interval (CI), 1.35–2.68]; p = 0.002). In addition, a model involving SMM was developed which predicted AEx (area under the curve: 0.750, 95% CI: 0.691–0.810). Conclusions: Low SMM was an independent risk factor for future AEx. Furthermore, a model involving SMM for predicting the risk of AEx in patients with asthma indicated that assessment of SMM has potential clinical implications for asthma management.
Minimal Hepatic Encephalopathy Affects Daily Life of Cirrhotic Patients: A Viewpoint on Clinical Consequences and Therapeutic Opportunities
Authors: Faccioli Jessica , Nardelli Silvia , Gioia Stefania , Riggio Oliviero , Ridola Lorenzo
Published: December 2022

Minimal hepatic encephalopathy (MHE) is a frequent complication of hepatic encephalopathy (HE) and can affect up to 80% of patients with liver cirrhosis. It is characterized by the lack of obvious clinical signs and the presence of alterations detectable using psychometric or electrophysiological testing focused on attention, working memory, psychomotor speed and visuospatial ability. Ideally, each patient should be tested for this condition because, despite the absence of symptoms, it has severe repercussions on daily life activities. It may be responsible for an inability to drive, sleep disturbances, risk of falls and inability to work. Some studies have highlighted its prognostically unfavorable role on mortality and risk of “overt” HE (OHE). Finally, MHE severely affects the lives of patients and caregivers, altering their quality of life and their socioeconomic status. Several treatments have been proposed for MHE treatment, including non-absorbable disaccharides, poorly absorbable antibiotics, such as rifaximin, probiotics and branched-chain amino acids, with promising results. For this reason, early diagnosis and intervention with appropriate measures is essential, with the aim of improving both performance on psychometric tests, as well as clinical aspects related to this condition.
Peripheral Blood Mononuclear Cells Mitochondrial Respiration and Superoxide Anion after Heart Transplantation
Authors: Alfatni Abrar , Charles Anne-Laure , Sauer François , Riou Marianne , Goupilleau Fabienne , Talha Samy , Meyer Alain , Andres Emmanuel , Kindo Michel , Mazzucotelli Jean-Philippe , Epailly Eric , Geny Bernard
Published: December 2022

Introduction: The mitochondrial function of circulating peripheral blood mononuclear cells (PBMCs) is an interesting new approach to cardiac diseases. Thus, PBMC’s mitochondrial respiration decreases in relation to heart failure severity. However, no data are available on heart-transplanted patients (Htx). Population and Methods: We determined PBMCs mitochondrial respiration by high-resolution respirometry (Oroboros Instruments) and superoxide anion production using electron paramagnetic resonance (Bruker-Biospin) in 20 healthy subjects and 20 matched Htx and investigated clinical, biological, echocardiographic, coronarography and biopsy characteristics. Results: PBMCs mitochondrial respiratory chain complex II respiration was decreased in Htx (4.69 ± 0.84 vs. 7.69 ± 1.00 pmol/s/million cell in controls and Htx patients, respectively; p = 0.007) and complex IV respiration was increased (24.58 ± 2.57 vs. 15.68 ± 1.67 pmol/s/million cell; p = 0.0035). Superoxide anion production was also increased in Htx (1.47 ± 0.10 vs. 1.15 ± 0.10 µmol/min; p = 0.041). The leucocyte-to-lymphocyte ratio was increased in Htx, whom complex II correlated with leucocyte number (r = 0.51, p = 0.02) and with the left ventricular posterior wall peak early diastolic myocardial velocity (r = −0.62, p = 0.005). Complex IV was increased in the two patients with acute rejection and correlated negatively with Htx’s isovolumetric relation time (r = −0.45, p = 0.045). Discussion: Although presenting with normal systolic function, Htx demonstrated abnormal PBMC’s mitochondrial respiration. Unlike immunosuppressive therapies, subclinical diastolic dysfunction might be involved in these changes. Additionally, lymphopenia might reduce complex II, and acute rejection enhances complex IV respirations. Conclusion: PBMC’s mitochondrial respiration appears modified in Htx, potentially linked to cellular shift, mild diastolic dysfunction and/or acute rejection.
Upper-Limb Disturbances in Female Patients with Poland Syndrome, including the Digit Ratio (2D:4D)
Authors: Fijałkowska Marta , Koziej Mateusz , Antoszewski Bogusław
Published: December 2022

Background: Poland syndrome (PS) is a rare congenital anomaly characterized by a variable clinical picture. Classic deformity consists of the unilateral hypoplasia or aplasia of the pectoralis major muscle and ipsilateral hand malformations. The aim of this study is to present disturbances in the development of upper limb in women with Poland syndrome (including digit ratio 2D:4D) in comparison to the healthy controls. Methods: The group of patients with Poland syndrome consisted of 36 women, while the control group consisted of 50 heathy women. Both upper limbs were measured anthropometrically. The length of all fingers and forearms were measured, and the digit ratio was calculated. Results: In women with Poland syndrome, the length of digits 2 and 4 and the forearm were significantly higher on the nonaffected side than on the affected side. In addition, there were significant differences between the length of digits 2 and 4 and the forearm between patients and controls. Conclusions: In patients with Poland syndrome, the upper limb at the affected side is significantly different from the upper limb on the healthy side, mainly in the length of the forearm and digits. While examining the patient with Poland syndrome, we think it is essential to pay attention not only to hand anomalies but also to the development of whole upper limb. It may prove to be helpful in estimating the complete picture of Poland syndrome.
Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis
Authors: Wierzbicki Piotr M. , Czajkowski Mateusz , Kotulak-Chrząszcz Anna , Bukowicz Justyna , Dzieciuch Klaudia , Sokołowska-Wojdyło Małgorzata , Kmieć Zbigniew , Matuszewski Marcin
Published: December 2022

Background. The nuclear factor–κB transcription factors 1 and 2 (NFKB1 and NFKB2) are key components of the NF-κB pathway, which responds to inflammatory signals. Since the NFKB1/2 factors are activated via different inflammatory molecules, we aimed to check their expression levels in penile cancer (PC), penile dermatoses: lichen sclerosus (PLS) and zoon balanitis (ZB). Methods: Skin biopsies from altered and healthy looking foreskin were obtained from 59 (49 LS; early PLS: 13, moderate PLS: 32, severe PLS: 4; 6 PC; 4 ZB) and unchanged foreskin from 13 healthy control adult males undergoing circumcision. NFKB1/2 mRNA levels were quantified by qPCR. Results: The highest levels of NFKB1 and NFKB2 were observed in PC, ca. 22 and 3.5 times higher than in control, respectively. NFKB1 expression was correlated with PLS progression (rs = 0.667) and was ca. 20 times higher in advanced PLS than in controls and early PLS. Occurrence of micro-incontinence was associated with elevated NFKB1 levels in PLS. Conclusion: This is the first study regarding gene profiles of NFKB1/2 in PC and penile dermatoses. New drugs targeting modulation of canonical-activated NF–κB pathway should be studied and introduced to the treatment of PLS and PC apart from other treatments.
Conduction System Pacing Today and Tomorrow
Authors: Haeberlin Andreas , Canello Siro , Kummer Andreas , Seiler Jens , Baldinger Samuel H. , Madaffari Antonio , Thalmann Gregor , Ryser Adrian , Gräni Christoph , Tanner Hildegard , Roten Laurent , Reichlin Tobias , Noti Fabian
Published: December 2022

Conduction system pacing (CSP) encompassing His bundle (HBP) and left bundle branch area pacing (LBBAP) is gaining increasing attention in the electrophysiology community. These relatively novel physiological pacing modalities have the potential to outperform conventional pacing approaches with respect to clinical endpoints, although data are currently still limited. While HBP represents the most physiological form of cardiac stimulation, success rates, bundle branch correction, and electrical lead performance over time remain a concern. LBBAP systems may overcome these limitations. In this review article, we provide a comprehensive overview of the current evidence, implantation technique, device programming, and follow-up considerations concerning CSP systems. Moreover, we discuss ongoing technical developments and future perspectives of CSP.
Mechanical, Thermal, and Acoustic Properties of Hemp and Biocomposite Materials: A Review
Authors: Dahal Raj Kumar , Acharya Bishnu , Dutta Animesh
Published: December 2022

Bio-based products are paving a promising path towards a greener future and helping win the fight against climate change and global warming mainly caused by fossil fuel consumption. This paper aims at highlighting the acoustic, thermal, and mechanical properties of hemp-based biocomposite materials. Change in sound absorption as a result of hemp fibers and hemp particle reinforcement are discussed in this paper. The thermal properties characterized by the thermal conductivity of the composites are also presented, followed by the mechanical properties and the current issues in biocomposite materials mainly containing hemp as a constituent element. Lastly, the effects of biofillers and biofibers on the various properties of the hemp-composite materials are discussed. This paper highlights the development of and issues in the field of hemp-based composite materials.
Influence of Steel Fiber and Carbon Fiber Mesh on Plastic Hinge Length of RCC Beams under Monotonic Loading
Authors: Sivanantham Pradeep , Pugazhlendi Deepak , Gurupatham Beulah Gnana Ananthi , Roy Krishanu
Published: December 2022

The most susceptible area of a structural member, where the most inelastic rotation would take place, is the plastic hinge. At this stage, flexural elements in particular achieve their maximal bending flexibility. This study uses finite element analysis (FEA) and experimental inquiry to analyze and test the effects of carbon fiber mesh jacketing and steel fiber reinforcement at the concrete beam’s plastic hinge length subjected to a vertical monotonic load. The compressive strength, split tensile strength, and flexural strength tests are used to evaluate the mechanical qualities, such as compressive strength and tensile strength, of M25 grade concrete that is used to cast specimens. While conducting this analysis, seven different parameters are taken into account. After the conventional concrete beam has been cast, the steel-fiber reinforced beam is cast. Several empirical formulas drawn from Baker, Sawyer, Corley, Mattock, Paulay, Priestley, and Park’s methods were used to calculate the length of the beam’s plastic hinge. Finally, the steel fiber was inserted independently at 150 mm into the concrete beam’s plastic hinge length mechanism using the techniques described by Paulay and Priestley. The analytical and experimental results are compared. The results obtained from the investigations by applying monotonic loads to the beam show that fibers used at specific plastic hinge lengths show a 41 kN ultimate load with 11.63 mm displacement, which is similar to that of conventional beam displacement, and performance. Meanwhile, the carbon fiber mesh wrapped throughout the beam behaves better than other members, showing an ultimate load of 64 kN with a 15.95 mm deflection. The fibers provided at the plastic hinge length of the beam perform similarly to those of a conventional beam; eventually, they become economical without sacrificing strength.
Numerical Model for a Geometrically Nonlinear Analysis of Beams with Composite Cross-Sections
Authors: Banić Damjan , Turkalj Goran , Kvaternik Simonetti Sandra , Lanc Domagoj
Published: December 2022

This paper presents a beam model for a geometrically nonlinear stability analysis of the composite beam-type structures. Each wall of the cross-section can be modeled with a different material. The nonlinear incremental procedure is based on an updated Lagrangian formulation where in each increment, the equilibrium equations are derived from the virtual work principle. The beam model accounts for the restrained warping and large rotation effects by including the nonlinear displacement field of the composite cross-section. First-order shear deformation theories for torsion and bending are included in the model through Timoshenko’s bending theory and a modified Vlasov’s torsion theory. The shear deformation coupling effects are included in the model using the six shear correction factors. The accuracy and reliability of the proposed numerical model are verified through a comparison of the shear-rigid and shear-deformable beam models in buckling problems. The obtained results indicated the importance of including the shear deformation effects at shorter beams and columns in which the difference that occurs is more than 10 percent.