bluuebunny/crossref_metadata_2025 huggingface dataset:
Parameter Estimation of Chopper Resistor in Medium-Voltage-Direct-Current during Grid Fault Ride through
Authors: Lee Hyeong-Jin , Kim Jin-Su , Kim Jae-Chul
Published: December 2018

Offshore wind farms with good wind quality are exponentially increasing. To take advantage of this, the offshore wind farms and the grid are connected using the MVDC. In the event of a grid fault, the traditional wind generators and the MVDC are disconnected from the grid fault to protect the devices. However, the wind generators and the MVDC must support the recovery of the grid fault because the disconnection of large capacity wind farms will cause a grid collapse. To prevent this problem, the LVRT requires maintaining the connection between the wind generators and the grid to contribute to the recovery of the grid fault. In this situation, the DC voltage of the MVDC rises due to the unbalanced power of the input and output. Several methods have been proposed to suppress the DC voltage rise of the MVDC. Among various methods, the CR is an effective method to suppress the DC voltage rise of the wind generators and the MVDC. However, the conventional CR designs only consider rated voltage and system capacity. Therefore, this paper proposes the parameter estimation of the CR considering the important factors. The proposed method is verified by PSCAD/EMTDC.
Submarine Landslides and their Distribution in the Gas Hydrate Area on the North Slope of the South China Sea
Authors: Wu Xuemin , Liang Qianyong , Ma Yun , Shi Yaohong , Xia Zhen , Liu Lihua , Haeckel Matthias
Published: December 2018

Integrated investigations have revealed abundant resources of gas hydrates on the northern slope of the South China Sea (SCS). Regarding the gas hydrate research of northern SCS, the gas hydrate related environment problem such as seabed landslides were also concentrated on in those areas. Based on 2D seismic data and sub-bottom profiles of the gas hydrate areas, submarine landslides in the areas of Qiongdongnan, Xisha, Shenhu, and Dongsha have been identified, characterized, and interpreted, and the geophysical characteristics of the northern SCS region investigated comprehensively. The results show 6 major landslides in the gas hydrate zone of the northern SCS and 24 landslides in the Shenhu and Dongsha slope areas of the northern SCS. The landslide zones are located mainly at water depths of 200–3000 m, and they occur on the sides of valleys on the slope, on the flanks of volcanoes, and on the uplifted steep slopes above magmatic intrusions. All landslides extend laterally towards the NE or NEE and show a close relationship to the ancient coastline and the steep terrain of the seabed. We speculate that the distribution and development of submarine landslides in this area has a close relationship with the tectonic setting and sedimentary filling characteristics of the slopes where they are located. Seismic activity is the important factor controlling the submarine landslide in Dongsha area, but the important factor controlling the submarine landslides in Shenhu area is the decomposition of natural gas hydrates.
Redirection Waveguide having Discrete Translational Symmetry for Photovoltaic Systems with Solar-Cell Units Placed at the Periphery
Authors: Ishibashi Akira , Kasai Tsuyoshi , Sawamura Nobuo
Published: December 2018

We have investigated a new type of redirection waveguides for concentration solar-cell systems. The solar cell units are to be placed in the peripheral region of the waveguide. The redirection waveguide converts three-dimensionally propagating (3D) photons into 2D photons that propagate in a planar waveguide which serves as the mainstream of the redirection waveguide. In this new type, the cladding layer on one side of the planar waveguide, not being spatially continuous, tangentially touches the core of the planar waveguide which, having an open geometry, is connected, through tributary waveguides, to the bottom plane where the 3D-photons come in vertically. Based on simulations, we have shown not only that good mainstream-to-mainstream propagation of light-wave can be obtained but also that the light propagation from the tributary waveguides to the mainstream is well done. We also have confirmed that light-waves from the tributary waveguides are well merged with those from the mainstream waveguide at the junction of the cores of the mainstream and the tributary waveguides. Light-waves injected from tributary waveguides propagates well in the core of the mainstream waveguide passing those junctions. The light-wave merging can be fulfilled in the waveguide with the discrete translational symmetry. Photons are shown to be well conveyed in the new waveguide losslessly for a distance of ~1 mm. The redirection waveguide is promising for the concentration photovoltaic systems with solar-cell units placed at its periphery.
High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.
Authors: Yang Xiong , Yang Xiaoyu , Guo Ting , Gao Kai , Zhao Tianyun , Chen Zhong , An Xinmin
Published: December 2018

Research Highlights: In the current study, we established a method for plant regeneration via somatic embryogenesis (SE) in Koelreuteria paniculata Laxm. for the first time. Background and Objectives: K. paniculata is an important ornamental and medicinal plant in China. However, the plant has difficulty with asexual reproduction, which imposes a limitation on large-scale propagation. Materials and Methods: Embryogenic calluses were induced from stems of aseptic seedlings on induction media. The effects of different media types and concentrations of N6-benzyladenine (BA), α-naphthaleneacetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D) on callus induction were examined. Embryogenic calluses were then transferred to Driver-Kuniyuki Walnut (DKW) media containing NAA (0.1–0.2 mg L−1) or 2,4-D (0.5–2.0 mg L−1) to develop somatic embryos. Cotyledon embryos were cultured on DKW media containing NAA (0.1–0.2 mg L−1) until maturation, and were then transferred to 1/2 DKW medium supplemented with 1.0 mg L−1 indole-3-butyric acid (IBA) to produce complete plants. The effects of IBA and NAA on rhizogenesis were then examined by clonal culture. Results: The maximum callus induction frequency (80.25%) was obtained on DKW medium supplemented by 0.5 mg L−1 BA, 0.25 mg L−1 NAA, and 1.5 mg L−1 2,4-D. NAA had a more pronounced effect on somatic embryo growth than did 2,4-D, with a maximum SE frequency (54.75%) observed with 0.1 mg L−1 NAA added to DKW medium. For clonal culture, the highest rooting rate (52%) was observed on 1/4 DKW medium containing 1.5 mg L−1 IBA. Histology studies confirmed the presence of embryogenic calluses and somatic embryos in different stages. Conclusions: This protocol provides a novel method for large-scale propagation of K. paniculata, and creates opportunities for genetic engineering in this species.
Variation in Climate Signals in Teak Tree-Ring Chronologies in Two Different Growth Areas
Authors: Preechamart Sineenart , Pumijumnong Nathsuda , Payomrat Paramate , Buajan Supaporn
Published: December 2018

We developed two tree-ring chronologies of teak (Tectona grandis L.f.) from Mae Tuen (462-year, 1555–2016) and Umphang (165-year, 1852–2016) in Tak province, northwestern Thailand. The chronologies were based on 67 and 71 living teak trees, respectively. We used crossdating methods to check and verify the tree-ring width data and tree-ring chronology construction using the ARSTAN program. In this study, the two teak tree-ring chronologies from two different growth areas could not be crossdated. The relationship among these chronologies is, thus, relatively low (r = 0.33, n = 165, p < 0.01). This result shows that the growth of tree-ring structure from two sites can be affected by a variety of non-climatic patterns due to site variation, such as topography, nutrient, light, and internal factors. However, these chronologies have a significant positive correlation with rainfall, during the pre-monsoon season (April to May). As demonstrated by the spatial correlation patterns, these chronologies represent April to May rainfall, which was a limiting factor of teak growth from northwestern Thailand. While the difference in surface temperatures of the Indian Ocean Dipole (IOD) might not be affected by rainfall, its unstable relationship with the El Niño-Southern Oscillation (ENSO) was noted to have occurred.
Bidirectional Recurrent Neural Network Approach for Arabic Named Entity Recognition
Authors: Ali Mohammed N. A. , Tan Guanzheng , Hussain Aamir
Published: December 2018

Recurrent neural network (RNN) has achieved remarkable success in sequence labeling tasks with memory requirement. RNN can remember previous information of a sequence and can thus be used to solve natural language processing (NLP) tasks. Named entity recognition (NER) is a common task of NLP and can be considered a classification problem. We propose a bidirectional long short-term memory (LSTM) model for this entity recognition task of the Arabic text. The LSTM network can process sequences and relate to each part of it, which makes it useful for the NER task. Moreover, we use pre-trained word embedding to train the inputs that are fed into the LSTM network. The proposed model is evaluated on a popular dataset called “ANERcorp.” Experimental results show that the model with word embedding achieves a high F-score measure of approximately 88.01%.
The Signaling Value of Punishing Norm-Breakers and Rewarding Norm-Followers
Authors: Adriani Fabrizio , Sonderegger Silvia
Published: December 2018

We formally explore the idea that punishment of norm-breakers may be a vehicle for the older generation to teach youngsters about social norms. We show that this signaling role provides sufficient incentives to sustain costly punishing behavior. People punish norm-breakers to pass information about past history to the younger generation. This creates a link between past, present, and future punishment. Information about the past is important for youngsters, because the past shapes the future. Reward-based mechanisms may also work and are welfare superior to punishment-based ones. However, reward-based mechanisms are fragile, since punishment is a more compelling signaling device (in a sense that we make precise).
Incidence, Costs and Predictors of Non-Union, Delayed Union and Mal-Union Following Long Bone Fracture
Authors: Ekegren Christina L. , Edwards Elton R. , De Steiger Richard , Gabbe Belinda J.
Published: December 2018

Fracture healing complications are common and result in significant healthcare burden. The aim of this study was to determine the rate, costs and predictors of two-year readmission for surgical management of healing complications (delayed, mal, non-union) following fracture of the humerus, tibia or femur. Humeral, tibial and femoral (excluding proximal) fractures registered by the Victorian Orthopaedic Trauma Outcomes Registry over five years (n = 3962) were linked with population-level hospital admissions data to identify two-year readmissions for delayed, mal or non-union. Study outcomes included hospital length-of-stay (LOS) and inpatient costs. Multivariable logistic regression was used to determine demographic and injury-related factors associated with admission for fracture healing complications. Of the 3886 patients linked, 8.1% were readmitted for healing complications within two years post-fracture, with non-union the most common complication and higher rates for femoral and tibial shaft fractures. Admissions for fracture healing complications incurred total costs of $4.9 million AUD, with a median LOS of two days. After adjusting for confounders, patients had higher odds of developing complications if they were older, receiving compensation or had tibial or femoral shaft fractures. Patients who are older, with tibial and femoral shaft fractures should be targeted for future research aimed at preventing complications.
Impact of the Family Environment on Juvenile Mental Health: eSports Online Game Addiction and Delinquency
Authors: Choi Chulhwan , Hums Mary A. , Bum Chul-Ho
Published: December 2018

Family types in Asian countries are rapidly changing as the society is changing. Thus, in this study, we analyzed and compared how the newly evolving family types (multicultural/dual-income) affect adolescents’ online game addiction, delinquency, and online gaming (eSports) participation motivation. Multiple regression analysis was performed to examine the causal relationships between the variables, and multivariate analysis of variance and analysis of variance were performed for comparative analyses. The results indicate that adolescents from dual-income families scored significantly higher on all factors related to juvenile delinquency and addiction factors (“salience”, “tolerance” and “withdrawal”). Additionally, adolescents from multicultural families revealed significantly higher scores on an addiction factor, “mood modification”. Lastly, adolescents in dual-income families were motivated to play online games to pass the time, and adolescents in multicultural families play online games to engage in social interaction. Results of this study may provide the answers required to help address societal issues related to adolescents in a changing society.
Treatment of Wastewater Using Seaweed: A Review
Authors: Arumugam Nithiya , Chelliapan Shreeshivadasan , Kamyab Hesam , Thirugnana Sathiabama , Othman Norazli , Nasri Noor Shawal
Published: December 2018

Inadequately treated or untreated wastewater greatly contribute to the release of unwanted toxic contaminants into water bodies. Some of these contaminants are persistent and bioaccumulative, becoming a great concern as they are released into the environment. Despite the abundance of wastewater treatment technologies, the adsorption method overall has proven to be an excellent way to treat wastewater from multiple industry sources. Because of its significant benefits, i.e., easy availability, handling, and higher efficiency with a low cost relative to other treatments, adsorption is opted as the best method to be used. However, biosorption using naturally found seaweeds has been proven to have promising results in removing pollutants, such as dyes from textile, paper, and the printing industry, nitrogen, and phosphorous and phenolic compounds, as well as heavy metals from various sources. Due to its ecofriendly nature together with the availability and inexpensiveness of raw materials, biosorption via seaweed has become an alternative to the existing technologies in removing these pollutants from wastewater effectively. In this article, the use of low-cost adsorbent (seaweed) for the removal of pollutants from wastewater has been reviewed. An extensive table summarises the applicability of seaweed in treating wastewater. Literature reported that the majority of research used simulated wastewater and minor attention has been given to biosorption using seaweed in the treatment of real wastewater.
Resilience and Poly-Victimization among Two Cohorts of Norwegian Youth
Authors: Huang Lihong , Mossige Svein
Published: December 2018

Previous research shows that there is a significant and positive relationship between being a victim of violence and experiencing high levels of psychological problems among young people. Conversely, resilience is negatively associated with psychological problems among young people in general, and this negative association is particularly strong among victims of violence. Our study examines resilience among young people (aged ≥ 18 years) who reported being victims of multiple forms of violence during childhood and adolescence using data from two national youth surveys administered in Norway in 2007 (N = 7033) and 2015 (N = 4531), respectively. We first compared the score of resilience, as measured by the Resilience Scale for Adolescents (READ), and the prevalence of poly-victimization, as identified by the number of young people in our study who were exposed to three of the four forms of violence (i.e., non-physical violence, witnessing violence against parents, physical violence, and sexual abuse). Second, we tested our hypothesis using our data and found that resilience—individuals’ capacity to handle adversity, as well as their use of social and cultural resources when facing adversity—moderates the association between poly-victimization and the onset of psychological problems.
Mushroom Poisoning—A 17 Year Retrospective Study at a Level I University Emergency Department in Switzerland
Authors: Keller Sarah A. , Klukowska-Rötzler Jolanta , Schenk-Jaeger Katharina M. , Kupferschmidt Hugo , Exadaktylos Aristomenis K. , Lehmann Beat , Liakoni Evangelia
Published: December 2018

The consequences of mushroom poisoning range from mild, mostly gastrointestinal, disturbances to organ failure or even death. This retrospective study describes presentations related to mushroom poisoning at an emergency department in Bern (Switzerland) from January 2001 to October 2017. Gastrointestinal disturbances were reported in 86% of the 51 cases. The National Poisons Information Centre and mycologists were involved in 69% and 61% of the cases, respectively. Identification of the mushroom type/family was possible in 43% of the cases. The most common mushroom family was Boletaceae (n = 21) and the most common mushrooms Xerocomus chrysenteron (n = 7; four being part of a cluster), Clitocybe nebularis, Lepista nuda and Lactarius semisanguifluus (n = 5 each, four being part of a cluster). Poisonous mushrooms included Amanita phalloides (n = 3, all analytically confirmed), Boletus satanas (n = 3), Amanita muscaria (n = 2) and Amanita pantherina (n = 2). There were no fatalities and 80% of the patients were discharged within 24 h. Mushroom poisoning does not appear to be a common reason for emergency consultation and most presentations were of minor severity and related to edible species (e.g., due to incorrect processing). Nevertheless, poisonous mushrooms and severe complications were also recorded. Collaboration with a poison centre and/or mycologists is of great importance, especially in high risk cases.
The Relationship between Free Press and Under-Reporting of Non-Fatal Occupational Injuries with Data from Representative National Indicators, 2015: Focusing on the Lethality Rate of Occupational Injuries among 39 Countries
Authors: Lim Sung-Shil , Yoon Jin-Ha , Rhie Jeongbae , Bae Suk Won , Kim Jihyun , Won Jong-Uk
Published: December 2018

The epidemiology of occupational injuries is reported worldwide, but suspicions of under-reporting prevail, probably associated with free press. We examined the association between freedom of the press and lethality rate of occupational injuries based on the most comprehensive International Labour Organization database on labour statistics (ILOSTAT) among 39 countries. The occupational injury indices, national indicators, and information on freedom of the press in 2015 were sourced from ILOSTAT, World Bank open data, World Health Organization and Freedom House. The lethality rate was the number of fatal occupational injuries per 10,000 total occupational injuries. The relationship among fatal and total occupation injury rates, lethality rate, and national statistics were analysed using Spearman’s rank correlation coefficients. Multivariable linear regression models with bootstrap estimation to manage non-normality determined freedom of the press associated with lethality rate. Freedom of the press was significantly correlated with fatal and total occupational injury rate and lethality rate of occupational injuries. Adjusting for national indicators, only freedom of the press was associated with lethality rate per 10,000 occupational injuries in the report of ILOSTAT. The lethality rate of occupational injury reported by each country might not reflect the actual lethality, but under-reported nonfatal occupational injuries, probably relating to freedom of the press.
“C.H.A.M.P. Families”: Description and Theoretical Foundations of a Paediatric Overweight and Obesity Intervention Targeting Parents—A Single-Centre Non-Randomised Feasibility Study
Authors: Reilly Kristen C. , Tucker Patricia , Irwin Jennifer D. , Johnson Andrew M. , Pearson Erin S. , Bock Dirk E. , Burke Shauna M.
Published: December 2018

Childhood obesity represents a significant global health challenge, and treatment interventions are needed. The purpose of this paper is to describe the components and theoretical model that was used in the development and implementation of a unique parent-focussed paediatric overweight/obesity intervention. C.H.A.M.P. Families was a single-centre, prospective intervention offered to parents of children aged between 6–14 years with a body mass index (BMI) ≥85th percentile for age and sex. The intervention included: (1) eight group-based (parent-only) education sessions over 13-weeks; (2) eight home-based activities; and (3) two group-based (family) follow-up support sessions. The first section of the manuscript contains a detailed description of each intervention component, as well as an overview of ongoing feasibility analyses. The theoretical portion details the use of evidence-based group dynamics principles and motivational interviewing techniques within the context of a broader social cognitive theory foundation. This paper provides researchers with practical examples of how theoretical constructs and evidence-based strategies can be applied in the development and implementation of parent-focussed paediatric obesity interventions. Given the need for transparent reporting of intervention designs and theoretical foundations, this paper also adds to the areas of implementation science and knowledge translation research.
Optimum Matching Model Using Long-Term Computing on Safer Rural Domestic Water Supply Based on Rainwater Harvesting
Authors: Jin Yan-Zhao , Zhou Lu-Wen , Lo Kwong Fai Andrew
Published: December 2018

A safe rural domestic water supply project has been initiated based on different consumption uses. Long-term computation method and the water balance principle are used to analyze the yearly water demand. Water supply and demand balance is achieved through regulated planning of the rainwater collection surface area and water storage capacity. The best combination of collection area and storage capacity is then determined for various rainfall zones in order to satisfy safe domestic water needs. Ultimately, an optimum matching model is developed to utilize rainwater harvesting for providing safe domestic water in rural areas.
Prenatal Exposure to Environmental Tobacco Smoke and Early Development of Children in Rural Guizhou Province, China
Authors: He Yang , Luo Renfu , Wang Tianyi , Gao Jingjing , Liu Chengfang
Published: December 2018

Background: There is a substantial body of evidence supporting the association between maternal active smoking during pregnancy and child development, but the association between prenatal exposure to environmental tobacco smoke (ETS) and early child development has not been well documented. This cross-sectional study examines the association between prenatal exposure to ETS and the development of children in their first two years of life. Methods: We interviewed the primary caregivers of 446 children under two years old in rural Guizhou Province, China. Based on self-reported assessments about whether the mother was exposed to ETS during pregnancy, we divided the children into the ETS-exposed group or the non-exposed group. Sociodemographic information was collected through a questionnaire. The cognitive, language, motor, and socioemotional abilities of children were assessed using the Bayley Scales of Infant Development III (BSID-III). A multivariate linear regression model adjusting for confounding variables was used to estimate the association of interest. Results: About 60% of mothers experienced ETS exposure during pregnancy. Cognitive and language scores were lower among children in the ETS-exposed group. When adjusting for characteristics of the child, the mother, the household, and village fixed effects, prenatal exposure to ETS was associated with lower cognition scores (−3.41; 95% confidence interval (CI): −6.39 to −0.42; p = 0.03) and language scores (−3.01; 95% CI: −5.39 to −0.09; p = 0.04). Frequency of prenatal exposure to ETS was also negatively associated with language development (−0.48; 95% CI: −0.87 to −0.09; p = 0.02) before children reached two years old. Conclusions: Prenatal exposure to ETS is negatively associated with the cognitive and language development of rural young children within their first two years of life. The government should take action to raise public awareness about the negative effects of tobacco use, with an emphasis on the protection of pregnant women and their children, in order to carry through comprehensive smoke-free laws in rural areas, while also increasing tobacco taxation.
Profiles of Basic Psychological Needs in Exercise Settings: An Examination of Differences in Contextual Motivation, Affect, and Achievement Goals
Authors: Kazak Zişan
Published: December 2018

Understanding leisure-time physical activity is vital for a healthy world. It is well known that physical activity has positive effects on psychological health, but further evidence is required to ascertain how different environments influence positive behavioral outcomes. Thus, the aim of this study was to examine the differences in contextual motivation, affect, and achievement goals according to profiles of basic psychological needs in adult exercisers. The sample consisted of 401 young adult exercisers ranging in age from 18 to 40 years from fitness centers in Izmir, Turkey. Participants completed measures of basic needs satisfaction, exercise motivations, trait affect, and achievement goals. Hierarchical cluster analysis, k-mean cluster analysis, multivariate analysis of variance, and post hoc analyses were performed. The results in this study revealed that the participants in Cluster 1, who were labeled as “very satisfied”, had higher scores than the other clusters in terms of positive behavioral outcomes. The results of this study revealed that greater satisfaction of the basic psychological needs leads to positive behavioral consequences in exercise contexts.
Concentrations, Distribution, Sources and Ecological Risk Assessment of Trace Elements in Soils from Wuhan, Central China
Authors: Tadesse Ababo Workineh , Gereslassie Tekleweini , Xu Qiang , Tang Xiaojun , Wang Jun
Published: December 2018

This study aimed to determine the concentration levels, potential sources and ecological risks of eleven trace elements, namely Cr, Fe, Co, Ni, Cu, As, Sb, Cd, Zn, Hg and Pb, in the soil from Huangpi district, Wuhan, Central China. Soil samples were collected from eighteen sites at soil depths of 1–10 and 10–20 cm and analyzed using Inductively Coupled Plasma-Mass Spectrometer ICP-MS (Thermo X SERIES 2, Scientific and Innovative Technology Co. Ltd., Beijing, China). The recorded mean concentration of the elements were in a decreasing order of Fe > Co > Cr > Ni > Pb > Cu > As > Cd > Sb > Zn > Hg. The mean concentration of trace elements, soil pH and total organic carbon (TOC) were higher at a soil depth of 1–10 cm. The obtained mean concentration of Cr, Co, As, Cd, Ni, Cu, Hg and Pb were above the soil background values of Wuhan and Hubei Province. The mean concentration values of Co, Ni and Cd, exceeded the recommended FAO (Food and Agriculture Organization)/ISRIC (International Soil Reference and Information Centre) (2004) and WHO/FAO (2001) values. Pearson’s correlation analysis illustrated that there was a strong and significant correlation between trace elements, whereas, a weak positive and negative correlation between elements and soil properties (pH and TOC). The principal component analysis (PCA) and cluster analysis (CA) result indicated that the concentration of trace elements in Huangpi soil were originated from anthropogenic sources. Potential ecological risk index (RI) of this study revealed that there is a high ecological risk of trace elements in the soil. Enrichment factor (EF) and geo-accumulation index (Igeo) of trace elements for this study indicated that the study area is strongly contaminated with Cd and Co. Generally, the finding of this research showed that Huangpi soil is contaminated.
Chronic Environmental and Occupational Lead Exposure and Kidney Function among African Americans: Dallas Lead Project II
Authors: Reilly Robert , Spalding Susan , Walsh Brad , Wainer Jeanne , Pickens Sue , Royster Marcene , Villanacci John , Little Bert B.
Published: December 2018

Background: We examined the effects of lead on kidney function in occupationally and environmentally exposed adults from a Dallas lead smelter community that was the site of an Environmental Protection Agency (EPA) Superfund clean-up. All subjects were African Americans—a racial group that bears a disproportionate burden of kidney disease. Methods: A two-phase health screening was conducted. Phase II included a physical examination and laboratory tests. Study subjects were African Americans residents, aged ≥19 years to ≤89 years. Of 778 subjects, 726 were environmentally exposed and 52 were both occupationally and environmentally exposed. The effects of lead exposure on estimated glomerular filtration rate (eGFR) were examined in three groups: male and female smelter-community residents, as well as males with both occupational and environmental exposure. Multiple linear regression was used to analyze the dependence of eGFR on log (blood lead level), duration of residence in the community, type 2 diabetes, and hypertension. Results: There was a statistically significant negative effect on kidney function for all three groups. Comparison of female and male residents showed a slightly larger negative effect of blood lead level on eGFR in females versus males, with the largest effect seen in male smelter-working residents. For each unit increase (log10 10 µg/dL = 1) in blood lead level, age-adjusted eGFR was reduced 21.2 mL/min/1.73 m2 in male residents, 25.3 mL/min/1.73 m2 in female residents and 59.2 mL/min/1.73 m2 in male smelter-working residents. Conclusions: Chronic lead exposure is associated with worsening kidney function in both African American male and female residents, as well as male workers in Dallas smelter communities. This effect is slightly, but not statistically significantly, worse in female residents than male residents, and significantly worse in males that both worked and resided in the smelter community.
On the Statistical Distribution of the Nonzero Spatial Autocorrelation Parameter in a Simultaneous Autoregressive Model
Authors: Luo Qing , Griffith Daniel A. , Wu Huayi
Published: December 2018

This paper focuses on the spatial autocorrelation parameter ρ of the simultaneous autoregressive model, and furnishes its sampling distribution for nonzero values, for two regular square (rook and queen) tessellations as well as a hexagonal case with rook connectivity, using Monte Carlo simulation experiments with a large sample size. The regular square lattice directly relates to increasingly used, remotely sensed images, whereas the regular hexagonal configuration is frequently used in sampling and aggregation situations. Results suggest an asymptotic normal distribution for estimated ρ. More specifically, this paper posits functions between ρ and its variance for three adjacency structures, which makes hypothesis testing implementable and furnishes an easily-computed version of the asymptotic variance for ρ at zero for each configuration. In addition, it also presents three examples, where the first employed a simulated dataset for a zero spatial autocorrelation case, and the other two used two empirical datasets—of these, one is a census block dataset for Wuhan (with a Moran coefficient of 0.53, allowing a null hypothesis of, e.g., ρ=0.7) to illustrate a moderate spatial autocorrelation case, and the other is a remotely sensed image of the Yellow Mountain region, China (with a Moran coefficient of 0.91, allowing a null hypothesis of, e.g., ρ=0.95) to illustrate a high spatial autocorrelation case.
Transcriptome and Hormone Comparison of Three Cytoplasmic Male Sterile Systems in Brassica napus
Authors: Ding Bingli , Hao Mengyu , Mei Desheng , Zaman Qamar U , Sang Shifei , Wang Hui , Wang Wenxiang , Fu Li , Cheng Hongtao , Hu Qiong
Published: December 2018

The interaction between plant mitochondria and the nucleus markedly influences stress responses and morphological features, including growth and development. An important example of this interaction is cytoplasmic male sterility (CMS), which results in plants producing non-functional pollen. In current research work, we compared the phenotypic differences in floral buds of different Brassica napus CMS (Polima, Ogura, Nsa) lines with their corresponding maintainer lines. By comparing anther developmental stages between CMS and maintainer lines, we identified that in the Nsa CMS line abnormality occurred at the tetrad stage of pollen development. Phytohormone assays demonstrated that IAA content decreased in sterile lines as compared to maintainer lines, while the total hormone content was increased two-fold in the S2 stage compared with the S1 stage. ABA content was higher in the S1 stage and exhibited a two-fold decreasing trend in S2 stage. Sterile lines however, had increased ABA content at both stages compared with the corresponding maintainer lines. Through transcriptome sequencing, we compared differentially expressed unigenes in sterile and maintainer lines at both (S1 and S2) developmental stages. We also explored the co-expressed genes of the three sterile lines in the two stages and classified these genes by gene function. By analyzing transcriptome data and validating by RT-PCR, it was shown that some transcription factors (TFs) and hormone-related genes were weakly or not expressed in the sterile lines. This research work provides preliminary identification of the pollen abortion stage in Nsa CMS line. Our focus on genes specifically expressed in sterile lines may be useful to understand the regulation of CMS.
A Polysaccharide Purified from Morchella conica Pers. Prevents Oxidative Stress Induced by H2O2 in Human Embryonic Kidney (HEK) 293T Cells
Authors: Xu Na , Lu Yi , Hou Jumin , Liu Chao , Sun Yonghai
Published: December 2018

Morchella conica Pers. (M. conica) has been used both as a medical and edible mushroom and possesses antimicrobial properties and antioxidant activities. However, the antioxidant properties of polysaccharides purified from M. conica have not been studied. The aim of this study was to investigate the in vitro antioxidant properties of a polysaccharide NMCP-2 (neutral M. conica polysaccharides-2) purified from M. conica, as determined by radical scavenging assay and H2O2-induced oxidative stress in HEK 293T cells. Results showed that NMCP-2 with an average molecular weight of 48.3 kDa possessed a much stronger chelating ability on ferrous ions and a higher ability to scavenge radical scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) than the other purified fraction of NMCP-1 from M. conica. Moreover, 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetra-zolium bromide (MTT) assay showed that NMCP-2 dose-dependently preserved cell viability of H2O2-induced cells. The NMCP-2 pretreated group reduced the generation of reactive oxygen species (ROS) content and increased the mitochondria membrane potential (MMP) levels. In addition, Hoechst 33342 staining revealed cells treated with NMCP-2 declined nuclear condensation. Ultrastructural observation revealed that NMCP-2 pretreatment alleviated the ruptured mitochondria when exposed to H2O2. Furthermore, western blot analysis showed that NMCP-2 prevented significant downregulation of the protein expression of Bax, cleaved caspases 3, and upregulated Bcl-2 levels. These results suggest the protective effects of NMCP-2 against H2O2-induced injury in HEK 293T cells. NMCP-2 could be used as a natural antioxidant of functional foods and natural drugs.
Evolutionary Analysis of Plastid Genomes of Seven Lonicera L. Species: Implications for Sequence Divergence and Phylogenetic Relationships
Authors: Liu Mi-Li , Fan Wei-Bing , Wang Ning , Dong Peng-Bin , Zhang Ting-Ting , Yue Ming , Li Zhong-Hu
Published: December 2018

Plant plastomes play crucial roles in species evolution and phylogenetic reconstruction studies due to being maternally inherited and due to the moderate evolutionary rate of genomes. However, patterns of sequence divergence and molecular evolution of the plastid genomes in the horticulturally- and economically-important Lonicera L. species are poorly understood. In this study, we collected the complete plastomes of seven Lonicera species and determined the various repeat sequence variations and protein sequence evolution by comparative genomic analysis. A total of 498 repeats were identified in plastid genomes, which included tandem (130), dispersed (277), and palindromic (91) types of repeat variations. Simple sequence repeat (SSR) elements analysis indicated the enriched SSRs in seven genomes to be mononucleotides, followed by tetra-nucleotides, dinucleotides, tri-nucleotides, hex-nucleotides, and penta-nucleotides. We identified 18 divergence hotspot regions (rps15, rps16, rps18, rpl23, psaJ, infA, ycf1, trnN-GUU-ndhF, rpoC2-rpoC1, rbcL-psaI, trnI-CAU-ycf2, psbZ-trnG-UCC, trnK-UUU-rps16, infA-rps8, rpl14-rpl16, trnV-GAC-rrn16, trnL-UAA intron, and rps12-clpP) that could be used as the potential molecular genetic markers for the further study of population genetics and phylogenetic evolution of Lonicera species. We found that a large number of repeat sequences were distributed in the divergence hotspots of plastid genomes. Interestingly, 16 genes were determined under positive selection, which included four genes for the subunits of ribosome proteins (rps7, rpl2, rpl16, and rpl22), three genes for the subunits of photosystem proteins (psaJ, psbC, and ycf4), three NADH oxidoreductase genes (ndhB, ndhH, and ndhK), two subunits of ATP genes (atpA and atpB), and four other genes (infA, rbcL, ycf1, and ycf2). Phylogenetic analysis based on the whole plastome demonstrated that the seven Lonicera species form a highly-supported monophyletic clade. The availability of these plastid genomes provides important genetic information for further species identification and biological research on Lonicera.
Skin Pigmentation: Is the Control of Melanogenesis a Target within Reach?
Authors: Napolitano Alessandra , Ito Shosuke
Published: December 2018

Skin pigmentation represents one of the most peculiar traits of human beings and its alteration as a consequence of pathological conditions has a dramatic impact on the wellness of individuals and their social relationships. [...]
Association between Plasma HMGB-1 and Silicosis: A Case-Control Study
Authors: Ma Jixuan , Zhou Yun , Li Wei , Xiao Lili , Yang Meng , Tan Qiyou , Xu Yiju , Chen Weihong
Published: December 2018

High-mobility group box-1 (HMGB-1) has been associated with fibrotic diseases. However, the role of HMGB-1 in silicosis is still uncertain. In this study, we conducted a case-control study involving 74 patients with silicosis and 107 age/gender-matched healthy controls in China. An Enzyme-linked immunosorbent assay (ELISA) was used to examine the concentrations of plasma HMGB-1 among all subjects. A logistic regression model and receiver operating characteristic curve (ROC) analysis were performed to assess the relationships between HMGB-1 and silicosis. We observed that plasma HMGB-1 concentrations were significantly increased in silicosis patients when compared with healthy controls (p < 0.05). Each 1 ng/mL increase in plasma HMGB-1 was positively associated with increased odds of silicosis, and the odds ratio (OR) (95% confidence interval) was 1.86 (1.52, 2.27). Additionally, compared with subjects with lower HMGB-1 concentrations, increased odds of silicosis were observed in those with higher HMGB-1 concentrations, and the OR was 15.33 (6.70, 35.10). Nonlinear models including a natural cubic spline function of continuous HMGB-1 yielded similar results. In ROC analyses, we found that plasma HMGB-1 >7.419 ng/mL had 81.6% sensitivity and 80.4% specificity for silicosis, and the area under the curve (AUC) was 0.84. Our results demonstrated that elevated plasma HMGB-1 was positivity associated with increased OR of silicosis.
Evolution from Natural β-Carboline Alkaloids to Obtain 1,2,4,9-tetrahydro-3-thia-9-aza-fluorene Derivatives as Potent Fungicidal Agents against Rhizoctonia solani
Authors: Xi Junmin , Zhang Zhijun , Zhu Qi , Zhong Guohua
Published: December 2018

Rice sheath blight, caused by Rhizoctonia solani, is a globally important rice disease and the increasing resistance of this pathogen highlights the need for new active compounds against rice sheath blight. In this study, natural β-carboline alkaloids were optimized to obtain a series of 1,2,4,9-tetrahydro-3-thia-9-aza-fluorene derivatives and evaluated for their fungicidal activity and mode of action against R. solani. Of these compounds, 18 exhibited significant in vitro fungicidal activity against R. solani, with an EC50 value of 2.35 μg/mL, and was more active than validamycin A. In vivo bioassay also demonstrated that 18 displayed superior protective and curative activities as compared to validamycin A. Mechanistically, 18 not only induced the loss of mitochondrial membrane potential and accumulation of reactive oxygen species, but also interfered with DNA synthesis. Therefore, compound 18 displayed pronounced in vitro and in vivo fungicidal activity against R. solani and could be used as a potential candidate for the control of rice sheath blight.
Role of Cytokinins in Senescence, Antioxidant Defence and Photosynthesis
Authors: Hönig Martin , Plíhalová Lucie , Husičková Alexandra , Nisler Jaroslav , Doležal Karel
Published: December 2018

Cytokinins modulate a number of important developmental processes, including the last phase of leaf development, known as senescence, which is associated with chlorophyll breakdown, photosynthetic apparatus disintegration and oxidative damage. There is ample evidence that cytokinins can slow down all these senescence-accompanying changes. Here, we review relationships between the various mechanisms of action of these regulatory molecules. We highlight their connection to photosynthesis, the pivotal process that generates assimilates, however may also lead to oxidative damage. Thus, we also focus on cytokinin induction of protective responses against oxidative damage. Activation of antioxidative enzymes in senescing tissues is described as well as changes in the levels of naturally occurring antioxidative compounds, such as phenolic acids and flavonoids, in plant explants. The main goal of this review is to show how the biological activities of cytokinins may be related to their chemical structure. New links between molecular aspects of natural cytokinins and their synthetic derivatives with antisenescent properties are described. Structural motifs in cytokinin molecules that may explain why these molecules play such a significant regulatory role are outlined.
Knockout of SlSBPASE Suppresses Carbon Assimilation and Alters Nitrogen Metabolism in Tomato Plants
Authors: Ding Fei , Hu Qiannan , Wang Meiling , Zhang Shuoxin
Published: December 2018

Sedoheptulose-1,7-bisphosphatase (SBPase) is an enzyme in the Calvin–Benson cycle and has been documented to be important in carbon assimilation, growth and stress tolerance in plants. However, information on the impact of SBPase on carbon assimilation and nitrogen metabolism in tomato plants (Solanum lycopersicum) is rather limited. In the present study, we investigated the role of SBPase in carbon assimilation and nitrogen metabolism in tomato plants by knocking out SBPase gene SlSBPASE using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing technology. Compared with wild-type plants, slsbpase mutant plants displayed severe growth retardation. Further analyses showed that knockout of SlSBPASE led to a substantial reduction in SBPase activity and as a consequence, ribulose-1,5-bisphosphate (RuBP) regeneration and carbon assimilation rate were dramatically inhibited in slsbpase mutant plants. It was further observed that much lower levels of sucrose and starch were accumulated in slsbpase mutant plants than their wild-type counterparts during the photoperiod. Intriguingly, mutation in SlSBPASE altered nitrogen metabolism as demonstrated by changes in levels of protein and amino acids and activities of nitrogen metabolic enzymes. Collectively, our data suggest that SlSBPASE is required for optimal growth, carbon assimilation and nitrogen metabolism in tomato plants.
Facile Preparation of Gold-Decorated Fe3O4 Nanoparticles for CT and MR Dual-Modal Imaging
Authors: Cai Jing , Miao Yu Qing , Li Li , Fan Hai Ming
Published: December 2018

The development of a multifunctional nanoprobe capable of non-invasive multimodal imaging is crucial for precise tumour diagnosis. Herein, we report a facile polymer-assisted method to produce Au-Fe3O4 nanocomposites (NCPs) for the dual-modal magnetic resonance (MR) and X-ray computed tomography (CT) imaging of tumours. In this approach, amino-functionalized Au nanospheres were first obtained by surface modification of the bifunctional polymer SH-PEG-NH2. Hydrophilic and carboxyl-functionalized Fe3O4 nanoparticles were produced by phase transfer of reverse micelle oxidation in our previous work. The Au nanoparticles were conjugated with hydrophilic Fe3O4 nanoparticles through an amide reaction. The obtained Au-Fe3O4 nanocomposites display a high r2 relativity (157.92 mM−1 s−1) and a Hounsfield units (HU) value (270 HU) at Au concentration of 8 mg/mL and could be applied as nanoprobes for the dual-modal MR/CT imaging of a xenografted tumour model. Our work provides a facile method to prepare Au-Fe3O4 nanocomposites for dual-modal MR/CT imaging, and this method can be extended to prepare other multifunctional nanoparticles for multimodal bioimaging.
Store-Operated Ca2+ Entry in Breast Cancer Cells: Remodeling and Functional Role
Authors: Jardin Isaac , Lopez Jose J. , Salido Gines M. , Rosado Juan A.
Published: December 2018

Breast cancer is the most common type of cancer in women. It is a heterogeneous disease that ranges from the less undifferentiated luminal A to the more aggressive basal or triple negative breast cancer molecular subtype. Ca2+ influx from the extracellular medium, but more specifically store-operated Ca2+ entry (SOCE), has been reported to play an important role in tumorigenesis and the maintenance of a variety of cancer hallmarks, including cell migration, proliferation, invasion or epithelial to mesenchymal transition. Breast cancer cells remodel the expression and functional role of the molecular components of SOCE. This review focuses on the functional role and remodeling of SOCE in breast cancer cells. The current studies suggest the need to deepen our understanding of SOCE in the biology of the different breast cancer subtypes in order to develop new and specific therapeutic strategies.