bluuebunny/crossref_metadata_2025 huggingface dataset:
Robotic Magnetorheological Finishing Technology Based on Constant Polishing Force Control
Authors: Zhang Lin , Zhang Chunlei , Fan Wei
Published: April 2022

The normal positioning error hinders the use of magnetorheological finishing (MRF) in robotic polishing. In this paper, the influence of robotic normal positioning error on the MRF removal rate is revealed, and a force-controlled end-effector for the robotic MRF process is presented. The developed end-effector is integrated into a six-axis industrial robot, and the robot positions the end-effector while the end-effector realizes the constant force control. A fused silicon mirror is polished, and the result shows that the proposed device effectively compensates for robotic normal positioning error and simultaneously maintains the stability of the polishing process. After deterministic polishing, the PV (peak to valley) of the figure is reduced from 126.56nm to 56.95nm, and the RMS (root mean square) is reduced from 22.15 nm to 7.59 nm.
Design Driven Research for Countryside Revitalization of a Rural Settlement of the Fujian Province, China
Authors: Semprebon Gerardo
Published: April 2022

Countryside development is receiving paramount attention in China, where political and cultural agendas promote rural revitalization as the core campaign to mitigate rural–urban disparities. The driving idea is that the rural can emancipate from its agricultural dependence to embrace more complex and integrated cycles of activities, such as leisure, health, productivity, market, and cultural services. The momentum reached by rural development in China has opened a new dimension for contemporary design culture, where the countryside has materialized as a frontier of architectural research. The paper synthesizes research by design experience carried out between 2017 and 2020 against the backdrop of the development program for a rural settlement of the Fujian Province, China. From the site reading to the experimental transformation proposal, the different phases offer sparks and arguments to put forward new understandings of designing in transitional rural contexts.
A Tale of Three Domes: The Un-Realized cupola of St Ignatius of Loyola in Roma
Authors: Spada Marco
Published: April 2022

The church of St Ignatius of Loyola in Rome, together with the Church of the Gesù, represents the most significant artistic contribution of the Jesuits in the Eternal City. Incorporated in the broader context of the Roman College, and built between 1626 and 1650 following a project by Padre (Father) Orazio Grassi S.J., it is the only one of the great Roman churches without a dome. The projects for the helioscopic dome by Orazio Grassi, the Cortonesque dome by Armando Brasini and the perspective dome by Andrea Pozzo represent the difficult attempt to create a perfectly rational layout for Rome, an ideal scientific and theological city. These projects tell the story of three different ways of conceiving architectural space affecting the city: political manifesto, imaginative introspection and colossal scenography. This paper describes the history of an “impossible” dome, analysing the historical evolution of the project and its three potential authors, three magnificent and idealist designers.
On a Family of Infinite Series with Reciprocal Catalan Numbers
Authors: Adegoke Kunle , Frontczak Robert , Goy Taras
Published: April 2022

We study a certain family of infinite series with reciprocal Catalan numbers. We first evaluate two special candidates of the family in closed form, where we also present some Catalan–Fibonacci relations. Then, we focus on the general properties of the family and prove explicit formulas, including two types of integral representations.
On an Integral Equation with the Riemann Function Kernel
Authors: Sitnik Sergei , Arian Abdul Ahad
Published: April 2022

This paper is concerned with a study of a special integral equation. This integral equation arises in many applied problems, including transmutation theory, inverse scattering problems, the solution of singular Sturm–Liouville and Shrödinger equations, and the representation of solutions of singular Sturm–Liouville and Shrödinger equations. A special integral equation is derived and formulated using the Riemann function of a singular hyperbolic equation. In the paper, the existence of a unique solution to this equation is proven by the method of successive approximations. The results can be applied, for example, to representations of solutions to Sturm–Liouville equations with singular potentials, such as Bargmann and Miura potentials, and similiar. The treatment of problems with such potentials are very important in mathematical physics, and inverse, scattering and related problems. The estimates received do not contain any undefined constants, and for transmutation kernels all estimates are explicitly written.
Demonstrating the Potential of Using Bio-Based Sustainable Polyester Blends for Bone Tissue Engineering Applications
Authors: Ramos-Rodriguez David H. , Pashneh-Tala Samand , Bains Amanpreet Kaur , Moorehead Robert D. , Kassos Nikolaos , Kelly Adrian L. , Paterson Thomas E. , Orozco-Diaz C. Amnael , Gill Andrew A. , Ortega Asencio Ilida
Published: April 2022

Healthcare applications are known to have a considerable environmental impact and the use of bio-based polymers has emerged as a powerful approach to reduce the carbon footprint in the sector. This research aims to explore the suitability of using a new sustainable polyester blend (Floreon™) as a scaffold directed to aid in musculoskeletal applications. Musculoskeletal problems arise from a wide range of diseases and injuries related to bones and joints. Specifically, bone injuries may result from trauma, cancer, or long-term infections and they are currently considered a major global problem in both developed and developing countries. In this work we have manufactured a series of 3D-printed constructs from a novel biopolymer blend using fused deposition modelling (FDM), and we have modified these materials using a bioceramic (wollastonite, 15% w/w). We have evaluated their performance in vitro using human dermal fibroblasts and rat mesenchymal stromal cells. The new sustainable blend is biocompatible, showing no differences in cell metabolic activity when compared to PLA controls for periods 1–18 days. FloreonTM blend has proven to be a promising material to be used in bone tissue regeneration as it shows an impact strength in the same range of that shown by native bone (just under 10 kJ/m2) and supports an improvement in osteogenic activity when modified with wollastonite.
In Vitro Cancer Models: A Closer Look at Limitations on Translation
Authors: Antunes Nina , Kundu Banani , Kundu Subhas C. , Reis Rui L. , Correlo Vítor
Published: April 2022

In vitro cancer models are envisioned as high-throughput screening platforms for potential new therapeutic discovery and/or validation. They also serve as tools to achieve personalized treatment strategies or real-time monitoring of disease propagation, providing effective treatments to patients. To battle the fatality of metastatic cancers, the development and commercialization of predictive and robust preclinical in vitro cancer models are of urgent need. In the past decades, the translation of cancer research from 2D to 3D platforms and the development of diverse in vitro cancer models have been well elaborated in an enormous number of reviews. However, the meagre clinical success rate of cancer therapeutics urges the critical introspection of currently available preclinical platforms, including patents, to hasten the development of precision medicine and commercialization of in vitro cancer models. Hence, the present article critically reflects the difficulty of translating cancer therapeutics from discovery to adoption and commercialization in the light of in vitro cancer models as predictive tools. The state of the art of in vitro cancer models is discussed first, followed by identifying the limitations of bench-to-bedside transition. This review tries to establish compatibility between the current findings and obstacles and indicates future directions to accelerate the market penetration, considering the niche market.
Biological Potential of Polyphenols in the Context of Metabolic Syndrome: An Analysis of Studies on Animal Models
Authors: Niewiadomska Joanna , Gajek-Marecka Aleksandra , Gajek Jacek , Noszczyk-Nowak Agnieszka
Published: April 2022

Metabolic syndrome (MetS) is a disease that has a complex etiology. It is defined as the co-occurrence of several pathophysiological disorders, including obesity, hyperglycemia, hypertension, and dyslipidemia. MetS is currently a severe problem in the public health care system. As its prevalence increases every year, it is now considered a global problem among adults and young populations. The treatment of choice comprises lifestyle changes based mainly on diet and physical activity. Therefore, researchers have been attempting to discover new substances that could help reduce or even reverse the symptoms when added to food. These attempts have resulted in numerous studies. Many of them have investigated the bioactive potential of polyphenols as a “possible remedy”, stemming from their antioxidative and anti-inflammatory effects and properties normalizing carbohydrate and lipid metabolism. Polyphenols may be supportive in preventing or delaying the onset of MetS or its complications. Additionally, the consumption of food rich in polyphenols should be considered as a supplement for antidiabetic drugs. To ensure the relevance of the studies on polyphenols’ properties, mechanisms of action, and potential human health benefits, researchers have used laboratory animals displaying pathophysiological changes specific to MetS. Polyphenols or their plant extracts were chosen according to the most advantageous mitigation of pathological changes in animal models best reflecting the components of MetS. The present paper comprises an overview of animal models of MetS, and promising polyphenolic compounds whose bioactive potential, effect on metabolic pathways, and supplementation-related benefits were analyzed based on in vivo animal models.
CRISPR/Cas9 Mediated Disruption of Seminal Fluid Protein Sfp62 Induces Male Sterility in Bombyx mori
Authors: Xu Xia , Chen Jine , Du Xin , Yao Lusong , Wang Yongqiang
Published: April 2022

Seminal fluid proteins provide factors necessary for development, storage, and activation of sperm. Altered expression of seminal fluid proteins can lead to defect in male infertility. We investigated the function of seminal fluid protein Sfp62 in the model lepidopteran insect Bombyx mori using CRISPR/Cas9 mediated mutagenesis. The knockout of BmSfp62 gene led to male sterility but has no effect on female fertility. The mutation did not affect growth and development of the silkworm of both sexes. Motility of sperm in male mutants was decreased and the mRNA expression levels of other genes encoding seminal fluid proteins were altered in BmSfp62 mutants compared to the wild-type controls. The male sterility caused by mutation of BmSfp62 was stably inherited. As the proteins encoded by Sfp62 genes are conserved among lepidopteran species, Sfp62 is a potential target for the biological management of lepidopteran pests.
Effects of “Bacuri” Seed Butter (Platonia insignis Mart.), a Brazilian Amazon Fruit, on Oxidative Stress and Diabetes Mellitus-Related Parameters in STZ-Diabetic Rats
Authors: Lindoso Jéssica Vanessa dos Santos , Alencar Salmon Rocha , Santos Andressa Amorim dos , Mello Neto Renato Sampaio , Mendes Ana Victória da Silva , Furtado Mariely Mendes , Silva Maisa Gomes da , Brito Ana Karolinne da Silva , Batista Emanuelle Karine Frota , Baêta Silvia de Araújo França , Moreira Nunes Paulo Humberto , Lucarini Massimo , Durazzo Alessandra , Arcanjo Daniel Dias Rufino , Martins Maria do Carmo de Carvalho e
Published: April 2022

This study aimed to investigate the effects of oral administration of Platonia insignis Mart. (“bacuri”) seed butter (BSB) on oxidative stress and diabetes mellitus-related parameters in streptozotocin-induced (STZ) diabetic rats. Diabetes mellitus was induced in female Wistar rats (180–250 g) by the intraperitoneal administration of STZ (45 mg/kg, b.w). BSB (25, 50, and 100 mg/kg) was administered to animals for four weeks. The effect on weight gain, food intake, blood glucose, glycated hemoglobin, hepatic transaminases, plasma and liver TBARS and MPO activity, erythrocyte SOD activity, non-protein sulfhydryl groups (SH-NP), and histopathology of the liver tissue was investigated. BSB at the dose of 100 mg/kg had a positive effect on the reduction in glycated hemoglobin percentage and increased albumin concentration, as well as decreased ALT and AST levels and increased SH-NP liver levels in treated animals compared to normal control rats. Moreover, BSB had no effects on weight gain, food intake, and fasting glucose. Thus, the BSB presented marked properties in improvement of hepatic antioxidant defenses, which demonstrates BSB as a potential hepatoprotective agent in metabolic disorders.
Transcriptome Profiling Reveals Differential Gene Expression of Laccase Genes in Aspergillus terreus KC462061 during Biodegradation of Crude Oil
Authors: Alharbi Nada K. , Alzaban Mayasar I. , Albarakaty Fawziah M. , Abd El-Aziz Abeer R. M. , AlRokban Ahlam H. , Mahmoud Mohamed A.
Published: April 2022

Fungal laccases have high catalytic efficiency and are utilized for the removal of crude oil because they oxidize various aliphatic and aromatic hydrocarbons and convert them into harmless compounds or less toxic compounds, thus accelerating the biodegradation potential of crude oil. Laccases are important gene families and the function of laccases genes varied widely based on transcription and function. Biodegradation of crude oil using Aspergillus terreus KC462061 was studied in the current study beside the transcription level of eight laccase (Lcc) genes have participated in biodegradation in the presence of aromatic compounds, and metal ions. Time-course profiles of laccase activity in the presence of crude oil indicated that the five inducers individual or combined have a very positive on laccase activity. In the status of the existence of crude oil, the synergistic effect of Cu-ABTS compound caused an increase in laccase yields up to 22-fold after 10 days than control. The biodegradation efficiencies of A. terreus KC462061 for aliphatic and aromatic hydrocarbons of crude oil were 82.1 ± 0.2% and 77.4 ± 0.6%, respectively. The crude oil biodegradation efficiency was improved by the supplemented Cu-ABTS compound in A. terreus KC462061. Gas chromatography–mass spectrometry was a very accurate tool to demonstrate the biodegradation efficiencies of A. terreus KC462061 for crude oil. Significant differences were observed in the SDS-PAGE of A. terreus KC462061 band intensities of laccase proteins after the addition of five inducers, but the Cu-ABTS compound highly affects very particular laccase electrophoresis. Quantitative real-time polymerase chain reaction (qPCR) was used for the analysis of transcription profile of eight laccase genes in A. terreus KC462061 with a verified reference gene. Cu2+ ions and Cu-ABTS were highly effective for efficient laccase expression profiling, mainly via Lcc11 and 12 transcription induction. The current study will explain the theoretical foundation for laccase transcription in A. terreus KC462061, paving the road for commercialization and usage.
Developments in Algorithms for Sequence Alignment: A Review
Authors: Chao Jiannan , Tang Furong , Xu Lei
Published: April 2022

The continuous development of sequencing technologies has enabled researchers to obtain large amounts of biological sequence data, and this has resulted in increasing demands for software that can perform sequence alignment fast and accurately. A number of algorithms and tools for sequence alignment have been designed to meet the various needs of biologists. Here, the ideas that prevail in the research of sequence alignment and some quality estimation methods for multiple sequence alignment tools are summarized.
The Impact of Prolonged Inflammation on Wound Healing
Authors: Holzer-Geissler Judith C. J. , Schwingenschuh Simon , Zacharias Martin , Einsiedler Johanna , Kainz Sonja , Reisenegger Peter , Holecek Christian , Hofmann Elisabeth , Wolff-Winiski Barbara , Fahrngruber Hermann , Birngruber Thomas , Kamolz Lars-Peter , Kotzbeck Petra
Published: April 2022

The treatment of chronic wounds still challenges modern medicine because of these wounds’ heterogenic pathophysiology. Processes such as inflammation, ischemia and bacterial infection play major roles in the progression of a chronic wound. In recent years, preclinical wound models have been used to understand the underlying processes of chronic wound formation. However, the wound models used to investigate chronic wounds often lack translatability from preclinical models to patients, and often do not take exaggerated inflammation into consideration. Therefore, we aimed to investigate prolonged inflammation in a porcine wound model by using resiquimod, a TLR7 and TLR8 agonist. Pigs received full thickness excisional wounds, where resiquimod was applied daily for 6 days, and untreated wounds served as controls. Dressing change, visual documentation and wound scoring were performed daily. Biopsies were collected for histological as well as gene expression analysis. Resiquimod application on full thickness wounds induced a visible inflammation of wounds, resulting in delayed wound healing compared to non-treated control wounds. Gene expression analysis revealed high levels of IL6, MMP1 and CD68 expression after resiquimod application, and histological analysis showed increased immune cell infiltration. By using resiquimod, we were able to show that prolonged inflammation delayed wound healing, which is often observed in chronic wounds in patients. The model we used shows the importance of inflammation in wound healing and gives an insight into the progression of chronic wounds.
A Small Molecule Promoting Neural Differentiation Suppresses Cancer Stem Cells in Colorectal Cancer
Authors: Choi Jung Kyu , Kwak Ihn-Sil , Yoon Sae-Bom , Cho Heeyeong , Moon Byoung-San
Published: April 2022

Cancer stem cells (CSCs) are a tumor cell subpopulation that drives tumor progression and metastasis, leading to a poor overall survival of patients. In colorectal cancer (CRC), the hyper-activation of Wnt/β-catenin signaling by a mutation of both adenomatous polyposis coli (APC) and K-Ras increases the size of the CSC population. We previously showed that CPD0857 inactivates Wnt/β-catenin signaling by promoting the ubiquitin-dependent proteasomal degradation of β-catenin and Ras proteins, thereby decreasing proliferation and increasing the apoptosis of CRC lines. CPD0857 also decreased the growth and invasiveness of CRC cells harboring mutant K-Ras resistant to EGFR mAb therapy. Here, we show that CPD0857 treatment decreases proliferation and increases the neuronal differentiation of neural progenitor cells (NPCs). CDP0857 effectively reduced the expression of CSC markers and suppressed self-renewal capacity. CPD0857 treatment also inhibited the proliferation and expression of CSC markers in D-K-Ras MT cells carrying K-Ras, APC and PI3K mutations, indicating the inhibition of PI3K/AKT signaling. Moreover, CPD0857-treated xenograft mice showed a regression of tumor growth and decreased numbers of CSCs in tumors. We conclude that CPD0857 could serve as the basis of a drug development strategy targeting CSCs activated through Wnt/β-catenin-Ras MAPK-PI3K/AKT signaling in CRCs.
HMTM-Mediated Enhancement of Brain Bioenergetics in a Mouse Tauopathy Model Is Blocked by Chronic Administration of Rivastigmine
Authors: Santos Renato X. , Melis Valeria , Goatman Elizabeth A. , Leith Michael , Baddeley Thomas C. , Storey John M. D. , Riedel Gernot , Wischik Claude M. , Harrington Charles R.
Published: April 2022

The tau protein aggregation inhibitor hydromethylthionine mesylate (HMTM) was shown recently to have concentration-dependent pharmacological activity in delaying cognitive decline and brain atrophy in phase 3 Alzheimer’s disease (AD) clinical trials; the activity was reduced in patients receiving symptomatic therapies. The methylthionine (MT) moiety has been reported to increase the clearance of pathological tau and to enhance mitochondrial activity, which is impaired in AD patients. In line 1 (L1) mice (a model of AD), HMTM (5/15 mg/kg) was administered either as a monotherapy or as an add-on to a chronic administration with the cholinesterase inhibitor rivastigmine (0.1/0.5 mg/kg) to explore mitochondrial function and energy substrate utilization as potential targets of drug interference. Compared with wild-type NMRI mice, the L1 mice accumulated greater levels of l-lactate and of the LDH-A subunit responsible for the conversion of pyruvate into l-lactate. In contrast, the levels of LDH-B and mitochondrial ETC subunits and the activity of complexes I and IV was not altered in the L1 mice. The activity of complex I and complex IV tended to increase with the HMTM dosing, in turn decreasing l-lactate accumulation in the brains of the L1 mice, despite increasing the levels of LDH-A. The chronic pre-dosing of the L1 mice with rivastigmine partially prevented the enhancement of the activity of complexes I and IV by HMTM and the increase in the levels of LDH-A while further reducing the levels of l-lactate. Thus, HMTM in combination with rivastigmine leads to a depletion in the energy substrate l-lactate, despite bioenergetic production not being favoured. In this study, the changes in l-lactate appear to be regulated by LDH-A, since neither of the experimental conditions affected the levels of LDH-B. The data show that HMTM monotherapy facilitates the use of substrates for energy production, particularly l-lactate, which is provided by astrocytes, additionally demonstrating that a chronic pre-treatment with rivastigmine prevented most of the HMTM-associated effects.
Strong and Elastic Chitosan/Silk Fibroin Hydrogels Incorporated with Growth-Factor-Loaded Microspheres for Cartilage Tissue Engineering
Authors: Min Qing , Tian Danlei , Zhang Yuchen , Wang Congcong , Wan Ying , Wu Jiliang
Published: April 2022

An emulsification method was developed for fabricating core-shell microspheres with a thick shell layer. Kartogenin (KGN) and platelet-derived growth factor BB (PDGF-BB) were respectively loaded into the core portion and the shell layer of the microspheres with high loading efficiency. The optimally built microspheres were combined with chitosan (CH) and silk fibroin (SF) to construct a new type of composite hydrogel with enhanced strength and elasticity, using genipin or/and tyrosinase as crosslinkers for the intended use in cartilage tissue engineering. The composite hydrogels were found to be thermo-responsive at physiological temperature and pH with well-defined injectability. Rheological measurements revealed that they had an elastic modulus higher than 6 kPa with a high ratio of elastic modulus to viscous modulus, indicative of their mechanically strong features. Compressive measurements demonstrated that they possessed well-defined elasticity. In addition, some gels had the ability to administer the temporal separation release of PDGF-BB and KGN in an approximately linear manner for several weeks. The released PDGF-BB was found to be bioactive based on its effects on Balb/c 3T3 cells. The composite gels supported the growth of seeded chondrocytes while preserving their phenotype. The results suggest that these composite gels have the potential for endogenous cartilage repair.
Association between Changes in White Matter Microstructure and Cognitive Impairment in White Matter Lesions
Authors: Hu An-Ming , Ma Yan-Ling , Li Yue-Xiu , Han Zai-Zhu , Yan Nan , Zhang Yu-Mei
Published: April 2022

This study investigated the characteristics of cognitive impairment in patients with white matter lesions (WMLs) caused by cerebral small vessel disease and the corresponding changes in WM microstructures. Diffusion tensor imaging (DTI) data of 50 patients with WMLs and 37 healthy controls were collected. Patients were divided into vascular cognitive impairment non-dementia and vascular dementia groups. Tract-based spatial statistics showed that patients with WMLs had significantly lower fractional anisotropy (FA) and higher mean diffusivity (MD), axial diffusivity (AD), and radial diffusivity (RD) values throughout the WM areas but predominately in the forceps minor, forceps major (FMA), bilateral corticospinal tract, inferior fronto-occipital fasciculus, superior longitudinal fasciculus, inferior longitudinal fasciculus (ILF), and anterior thalamic radiation, compared to the control group. These fiber bundles were selected as regions of interest. There were significant differences in the FA, MD, AD, and RD values (p < 0.05) between groups. The DTI metrics of all fiber bundles significantly correlated with the Montreal Cognitive Assessment (p < 0.05), with the exception of the AD values of the FMA and ILF. Patients with WMLs showed changes in diffusion parameters in the main WM fiber bundles. Quantifiable changes in WM microstructure are the main pathological basis of cognitive impairment, and may serve as a biomarker of WMLs.
Equivalent Dynamic Load Factor of Different Non-Exceedance Probability for Crowd Jumping Loads
Authors: Xiong Jiecheng , Duan Shuqian , Qian Hui , Pan Ziye
Published: April 2022

Existing dynamic load factors (DLF) for crowd jumping loads are modeled by identifying the peaks and energies from the corresponding Fourier amplitude spectrum, which may underestimate and overestimate structural responses, respectively. Based on the principle of equal structural responses, this study herein develops an equivalent DLF, using the frequency response function to weight and integrate the power spectral density (PSD) matrix of crowd jumping loads. Firstly, massive PSD matrices of different crowd sizes and different metronome frequencies are simulated using a random field model of crowd jumping loads. Thereafter, the equivalent DLF of different structural damping ratios, crowd sizes, metronome frequencies, and non-exceedance probabilities are investigated, and a model of the equivalent DLF is established accordingly. It is believed that this model could provide an efficient and accurate way to conduct reliability design for structures subjected to crowd jumping loads.
Prediction of Bond-Slip Behavior of Circular/Squared Concrete-Filled Steel Tubes
Authors: Allouzi Rabab A. , Almasaeid Hatem H. , Salman Donia G. , Abendeh Raed M. , Rabayah Hesham S.
Published: April 2022

Numerous existing formulas predicted the ultimate interfacial bond strength in concrete-filled steel tubes (CFST) between steel tubes and concrete core without investigating the whole response under push-out load. In this research, four models are proposed to predict the interfacial behavior in CFST including the post-peak branch under the push-out loading test based on 157 circular specimens and 105 squared specimens from the literature. Two models (one for circular and one for squared CFST) are developed and calibrated using artificial neural network (ANN) and two models (one for circular and one for squared CFST) are developed based on multivariable regression analysis, analysis of variance (ANOVA). The shape of the specimen (circular or squared), diameter of the tube, thickness of the tube, concrete compressive strength, age at the time of testing, and length of the specimen are the main factors considered. These models are then compared to other existing formulas to verify their capability to better predict the ultimate interfacial bond strength. It is found that the ANN model gives better results for most of the considered data. It is also found that ANN models can predict the overall bond-slip response for the considered dataset. In order to simulate the response of any CFST column using finite element (FE) method, it is vital to have sufficient input data on the overall bond-slip behavior between the interior face of the steel tube and the exterior surface of the concrete core including the post-peak branch. Accordingly, the suggested ANN model is used to generate the required input data related to the cohesive behavior and damage along the interface in ABAQUS model to simulate the response of two circular and two squared CFST columns under concentric compressive load. The results are in good agreement with experimental outcomes. The cohesive criterion and damage interface that are used based on ANN models in FE are found to be sufficient and can be adopted to model CFST columns.
Systemic Therapy De-Escalation in Early-Stage Triple-Negative Breast Cancer: Dawn of a New Era?
Authors: Gupta Ravi Kumar , Roy Arya Mariam , Gupta Ashish , Takabe Kazuaki , Dhakal Ajay , Opyrchal Mateusz , Kalinski Pawel , Gandhi Shipra
Published: April 2022

Early-stage triple negative breast cancer (TNBC) has been traditionally treated with surgery, radiation, and chemotherapy. The current standard of care systemic treatment of early-stage II and III TNBC involves the use of anthracycline-cyclophosphamide and carboplatin-paclitaxel with pembrolizumab in the neoadjuvant setting followed by adjuvant pembrolizumab per KEYNOTE-522. It is increasingly clear that not all patients with early-stage TNBC need this intensive treatment, thus paving the way for exploring opportunities for regimen de-escalation in selected subgroups. For T1a tumors (≤5 mm), chemotherapy is not used, and for tumors 6–10 mm (T1b) in size with negative lymph nodes, retrospective studies have failed to show a significant benefit with chemotherapy. In low-risk patients, anthracycline-free chemotherapy may be as effective as conventional therapy, as shown in some studies where replacing anthracyclines with carboplatin has shown non-inferior results for pathological complete response (pCR), which may form the backbone of future combination therapies. Recent advances in our understanding of TNBC heterogeneity, mutations, and surrogate markers of response such as pCR have enabled the development of multiple treatment options in the (neo)adjuvant setting in order to de-escalate treatment. These de-escalation studies based on tumor mutational status, such as using Poly ADP-ribose polymerase inhibitors (PARPi) in patients with BRCA mutations, and new immunotherapies such as PD1 blockade, have shown a promising impact on pCR. In addition, the investigational use of (bio)markers, such as high levels of tumor-infiltrating lymphocytes (TILs), low levels of tumor-associated macrophages (TAMs), and complete remission on imaging, also look promising. In this review, we cover the current standard of care systemic treatment of early TNBC and review the opportunities for treatment de-escalation based on clinical risk factors, biomarkers, mutational status, and molecular subtype.
Alternatives for MRI in Prostate Cancer Diagnostics—Review of Current Ultrasound-Based Techniques
Authors: Gurwin Adam , Kowalczyk Kamil , Knecht-Gurwin Klaudia , Stelmach Paweł , Nowak Łukasz , Krajewski Wojciech , Szydełko Tomasz , Małkiewicz Bartosz
Published: April 2022

The purpose of this review is to present the current role of ultrasound-based techniques in the diagnostic pathway of prostate cancer (PCa). With overdiagnosis and overtreatment of a clinically insignificant PCa over the past years, multiparametric magnetic resonance imaging (mpMRI) started to be recommended for every patient suspected of PCa before performing a biopsy. It enabled targeted sampling of the suspicious prostate regions, improving the accuracy of the traditional systematic biopsy. However, mpMRI is associated with high costs, relatively low availability, long and separate procedure, or exposure to the contrast agent. The novel ultrasound modalities, such as shear wave elastography (SWE), contrast-enhanced ultrasound (CEUS), or high frequency micro-ultrasound (MicroUS), may be capable of maintaining the performance of mpMRI without its limitations. Moreover, the real-time lesion visualization during biopsy would significantly simplify the diagnostic process. Another value of these new techniques is the ability to enhance the performance of mpMRI by creating the image fusion of multiple modalities. Such models might be further analyzed by artificial intelligence to mark the regions of interest for investigators and help to decide about the biopsy indications. The dynamic development and promising results of new ultrasound-based techniques should encourage researchers to thoroughly study their utilization in prostate imaging.
Biorecognition Engineering Technologies for Cancer Diagnosis: A Systematic Literature Review of Non-Conventional and Plausible Sensor Development Methods
Authors: Mayoral-Peña Kalaumari , González Peña Omar Israel , Orrantia Clark Alexia María , Flores-Vallejo Rosario del Carmen , Oza Goldie , Sharma Ashutosh , De Donato Marcos
Published: April 2022

Cancer is the second cause of mortality worldwide. Early diagnosis of this multifactorial disease is challenging, especially in populations with limited access to healthcare services. A vast repertoire of cancer biomarkers has been studied to facilitate early diagnosis; particularly, the use of antibodies against these biomarkers has been of interest to detect them through biorecognition. However, there are certain limitations to this approach. Emerging biorecognition engineering technologies are alternative methods to generate molecules and molecule-based scaffolds with similar properties to those presented by antibodies. Molecularly imprinted polymers, recombinant antibodies, and antibody mimetic molecules are three novel technologies commonly used in scientific studies. This review aimed to present the fundamentals of these technologies and address questions about how they are implemented for cancer detection in recent scientific studies. A systematic analysis of the scientific peer-reviewed literature regarding the use of these technologies on cancer detection was carried out starting from the year 2000 up to 2021 to answer these questions. In total, 131 scientific articles indexed in the Web of Science from the last three years were included in this analysis. The results showed that antibody mimetic molecules technology was the biorecognition technology with the highest number of reports. The most studied cancer types were: multiple, breast, leukemia, colorectal, and lung. Electrochemical and optical detection methods were the most frequently used. Finally, the most analyzed biomarkers and cancer entities in the studies were carcinoembryonic antigen, MCF-7 cells, and exosomes. These technologies are emerging tools with adequate performance for developing biosensors useful in cancer detection, which can be used to improve cancer diagnosis in developing countries.
Development of Monodisperse Mesoporous Microballs Composed of Decahedral Anatase Nanocrystals
Authors: Chang Ying , Jiang Jiayi , Wei Zhishun , Kowalska Ewa
Published: April 2022

Mesoporous monodisperse microballs of amorphous titania were prepared from solution of absolute ethanol, tetrabutyl titanate (TBOT) and potassium chloride via a sub-zero sol–gel route. The as-obtained microballs were used as the precursor in an alcohothermal (ethanol with a small amount of water) process to synthesize monodisperse mesoporous microballs built of decahedral anatase nanocrystals. FE-SEM observation and XRD analysis have confirmed that the formed decahedral anatase-rich powder retained the original spherical morphology of the precursor. Importantly, a hierarchical structure composed of faceted anatase has been achieved under “green” conditions, i.e., fluorine-free. Additionally, the hysteresis loops (BET results) have confirmed the existence of mesopores. Interestingly, faceted microballs show noticeable photocatalytic activity under UV/vis irradiation for hydrogen generation without any co-catalyst use, reaching almost forty times higher activity than that by famous commercial titania photocatalyst—P25. It has been proposed that enhanced photocatalytic performance is caused by mesoporous structure and co-existence of two kinds of facets, i.e., {001} and {101}, and thus hindered charge carriers’ recombination.
Hydrocarbon Oxidation Depth: H2O2/Cu2Cl4·2DMG/CH3CN System
Authors: Shchapin Igor Yu. , Nekhaev Andrey I. , Ramazanov Dzhamalutdin N. , Al-Yusufi Mohammed , Samoilov Vadim O. , Maximov Anton L.
Published: April 2022

The oxidation of hydrocarbons of different structures under the same conditions is an important stage in the study of the chemical properties of both the hydrocarbons themselves and the oxidation catalysts. In a 50% H2O2/Cu2Cl4·2DMG/CH3CN system, where DMG is dimethylglyoxime (Butane-2,3-dione dioxime), at 50 °C under the same or similar conditions, we oxidized eleven RH hydrocarbons of different structures: mono-, bi- and tri-cyclic, framework and aromatic. To compare the composition of the oxidation products of these hydrocarbons, we introduced a new quantitative characteristic, “distributive oxidation depth D(O), %” and showed the effectiveness of its application. The adiabatic ionization potentials (AIP) and the vertical ionization potentials (VIP) of the molecules of eleven oxidized and related hydrocarbons were calculated using the DFT method in the B3LYP/TZVPP level of theory for comparison with experimental values and correlation with D(O). The same calculations of AIP were made for the molecules of the oxidant, solvent, DMG, related compounds and products. It is shown that component X, which determines the mechanism of oxidation of hydrocarbons RH with AIP(Exp) ≥ AIP(X) = 8.55 ± 0.03 eV, is a trans-DMG molecule. Firstly theoretically estimated experimental values of AIP(trans-DMG) = 8.53 eV and AIP(cis-DMG) = 8.27 eV.
Carbon Formation in the Reforming of Simulated Biomass Gasification Gas on Nickel and Rhodium Catalysts
Authors: Kihlman Johanna , Simell Pekka
Published: April 2022

Biomass gasification gas contains hydrocarbons that must be converted to CO and H2 prior to the utilization of the gas in a synthesis unit. Autothermal or steam reforming operating with a nickel or noble metal catalyst is a feasible option to treat the gas, but the harsh reaction conditions may lead to the formation of solid carbon. This study discusses the effects of pressure, time-on-stream, and ethylene content on the carbon formation on nickel and rhodium catalysts. The experiments were carried out with laboratory-scale equipment using reaction conditions that were closely simulated after a pilot-scale biomass gasifier. The results indicated that ethylene content above 20,000 vol-ppm and the increased pressure would increase the carbon formation, although there were differences between the rhodium and nickel catalysts. However, carbon formation was significantly more pronounced on the nickel catalyst when the reaction time was increased from 5 h to 144 h. The type of carbon was found to be primarily encapsulating and graphitic. The formation of whisker carbons (also known as carbon nanotubes) was not observed, which is consistent with the literature as the feed gas contained H2S. It was concluded that utilizing a noble metal catalyst as the front layer of the catalyst bed could lower the risk for carbon formation sufficiently to provide stable long-term operation.
Distinct Dominant Lineage from In Vitro Expanded Adipose-Derived Stem Cells (ASCs) Exhibits Enhanced Wound Healing Properties
Authors: Peng Qiuyue , Ren Guoqiang , Xuan Zongzhe , Duda Martyna , Pennisi Cristian Pablo , Porsborg Simone Riis , Fink Trine , Zachar Vladimir
Published: April 2022

It has been suggested that immunophenotypically defined lineages within the in vitro expanded adipose-derived stem cell (ASC) may play a beneficial role from the perspective of a personalized intervention. Therefore, to better understand the implications of different surface marker profiles for the functionality, we set out to examine the evolution of ASC-variants based on the co-expression of five bright or eight dim epitopes. At passages P1, P4, and P8, the co-localization of five bright markers (CD73, CD90, CD105, CD166, and CD201), or eight dim markers (CD34, CD36, CD200, CD248, CD271, CD274, CD146, and the Stro-1), was investigated by flow cytometry. Selected subpopulations were isolated using the fluorescence-activated cells sorting from the cryopreserved P4 and analyzed in terms of proliferative and clonogenic properties, trilineage differentiation, and wound healing potential. Only two of the dim epitopes were found in representative subpopulations (SP), and from the P4 onwards, two major combinations featuring the CD274+ (SP1) or the CD274+ CD146+ (SP2) emerged. Upon sorting and growth, both subpopulations assumed new but highly similar clonal profiles, consisting of the CD274+ CD146+ and the CD274+ CD146+ CD248+ phenotypes. The functional analysis revealed that the SP2 surpassed SP1 and the unfractionated cells regarding the growth rate, clonogenic activity, and the wound closure and endothelial tube formation potential. The surface epitopes may be considered a tool to enrich specific functionality and thus improve therapeutic outcomes in dedicated circumstances.
Microglial Endocannabinoid Signalling in AD
Authors: Scipioni Lucia , Ciaramellano Francesca , Carnicelli Veronica , Leuti Alessandro , Lizzi Anna Rita , De Dominicis Noemi , Oddi Sergio , Maccarrone Mauro
Published: April 2022

Chronic inflammation in Alzheimer’s disease (AD) has been recently identified as a major contributor to disease pathogenesis. Once activated, microglial cells, which are brain-resident immune cells, exert several key actions, including phagocytosis, chemotaxis, and the release of pro- or anti-inflammatory mediators, which could have opposite effects on brain homeostasis, depending on the stage of disease and the particular phenotype of microglial cells. The endocannabinoids (eCBs) are pleiotropic bioactive lipids increasingly recognized for their essential roles in regulating microglial activity both under normal and AD-driven pathological conditions. Here, we review the current literature regarding the involvement of this signalling system in modulating microglial phenotypes and activity in the context of homeostasis and AD-related neurodegeneration.
The Influence of Gut Dysbiosis in the Pathogenesis and Management of Ischemic Stroke
Authors: Chidambaram Saravana Babu , Rathipriya Annan Gopinath , Mahalakshmi Arehally M. , Sharma Sonali , Hediyal Tousif Ahmed , Ray Bipul , Sunanda Tuladhar , Rungratanawanich Wiramon , Kashyap Rajpal Singh , Qoronfleh M. Walid , Essa Musthafa Mohamed , Song Byoung-Joon , Monaghan Tanya M.
Published: April 2022

Recent research on the gut microbiome has revealed the influence of gut microbiota (GM) on ischemic stroke pathogenesis and treatment outcomes. Alterations in the diversity, abundance, and functions of the gut microbiome, termed gut dysbiosis, results in dysregulated gut–brain signaling, which induces intestinal barrier changes, endotoxemia, systemic inflammation, and infection, affecting post-stroke outcomes. Gut–brain interactions are bidirectional, and the signals from the gut to the brain are mediated by microbially derived metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs); bacterial components, such as lipopolysaccharide (LPS); immune cells, such as T helper cells; and bacterial translocation via hormonal, immune, and neural pathways. Ischemic stroke affects gut microbial composition via neural and hypothalamic–pituitary–adrenal (HPA) pathways, which can contribute to post-stroke outcomes. Experimental and clinical studies have demonstrated that the restoration of the gut microbiome usually improves stroke treatment outcomes by regulating metabolic, immune, and inflammatory responses via the gut–brain axis (GBA). Therefore, restoring healthy microbial ecology in the gut may be a key therapeutic target for the effective management and treatment of ischemic stroke.
Total and Mitochondrial Transcriptomic and Proteomic Insights into Regulation of Bioenergetic Processes for Shoot Fast-Growth Initiation in Moso Bamboo
Authors: Wang Xiaojing , Geng Xin , Yang Lilin , Chen Yuzhen , Zhao Zhiheng , Shi Weijia , Kang Lan , Wu Ruihua , Lu Cunfu , Gao Jian
Published: April 2022

As a fast-growing, woody grass plant, Moso bamboo (Phyllostachys edulis) can supply edible shoots, building materials, fibrous raw material, raw materials for crafts and furniture and so on within a relatively short time. Rapid growth of Moso bamboo occurs after the young bamboo shoots are covered with a shell and emerge from the ground. However, the molecular reactions of bioenergetic processes essential for fast growth remain undefined. Herein, total and mitochondrial transcriptomes and proteomes were compared between spring and winter shoots. Numerous key genes and proteins responsible for energy metabolism were significantly upregulated in spring shoots, including those involved in starch and sucrose catabolism, glycolysis, the pentose phosphate pathway, the tricarboxylic acid cycle and oxidative phosphorylation. Accordingly, significant decreases in starch and soluble sugar, higher ATP content and higher rates of respiration and glycolysis were identified in spring shoots. Further, the upregulated genes and proteins related to mitochondrial fission significantly increased the number of mitochondria, indirectly promoting intracellular energy metabolism. Moreover, enhanced alternate-oxidase and uncoupled-protein pathways in winter shoots showed that an efficient energy-dissipating system was important for winter shoots to adapt to the low-temperature environment. Heterologous expression of PeAOX1b in Arabidopsis significantly affected seedling growth and enhanced cold-stress tolerance. Overall, this study highlights the power of comparing total and mitochondrial omics and integrating physiochemical data to understand how bamboo initiates fast growth through modulating bioenergetic processes.
Effects of Oxysterols on Immune Cells and Related Diseases
Authors: de Freitas Fábio Alessandro , Levy Débora , Reichert Cadiele Oliana , Cunha-Neto Edecio , Kalil Jorge , Bydlowski Sérgio Paulo
Published: April 2022

Oxysterols are the products of cholesterol oxidation. They have a wide range of effects on several cells, organs, and systems in the body. Oxysterols also have an influence on the physiology of the immune system, from immune cell maturation and migration to innate and humoral immune responses. In this regard, oxysterols have been involved in several diseases that have an immune component, from autoimmune and neurodegenerative diseases to inflammatory diseases, atherosclerosis, and cancer. Here, we review data on the participation of oxysterols, mainly 25-hydroxycholesterol and 7α,25-dihydroxycholesterol, in the immune system and related diseases. The effects of these oxysterols and main oxysterol receptors, LXR and EBI2, in cells of the immune system (B cells, T cells, macrophages, dendritic cells, oligodendrocytes, and astrocytes), and in immune-related diseases, such as neurodegenerative diseases, intestinal diseases, cancer, respiratory diseases, and atherosclerosis, are discussed.
Orthorexia and Eating Disorders in Adolescents and Young Adults: A Systematic Review
Authors: Skella Panagiota , Chelmi Maria Eleni , Panagouli Eleni , Garoufi Anastasia , Psaltopoulou Theodora , Mastorakos George , Sergentanis Theodoros N. , Tsitsika Artemis
Published: April 2022

Background: In recent years a new term in the field of eating behaviors has emerged, namely “orthorexia”. This behavior is associated with significant dietary restrictions and omission of food groups. The aim of the present study is to estimate the possible correlations between orthorexia and eating disorders in young adults and adolescents. Methods: A systematic review of related articles in PubMed, Google Scholar, and PsycInfo was conducted up to 30 June 2021. Results: A total of 37 studies (16,402 subjects) were considered eligible for this systematic review. Significant correlations were observed in most of the studies between orthorexia and eating disorders. However, the majority of studies adopted a cross-sectional design. Conclusions: An association between and eating disorders emerged. Prospective studies seem necessary to investigate associations and succession of orthorexia and eating disorders over time.