bluuebunny/crossref_metadata_2025 huggingface dataset:
Al-modification for PS-PVD 7YSZ TBCs to improve particle erosion and thermal cycle performances
Authors: Zhang Xiaofeng , Li Ming , Zhang Ao , Guo Shuangquan , Mao Jie , Deng Chunming , Wang Panpan , Deng Changguang , Feng Junli , Liu Min , Zhou Kesong , Lai Cheng
Published: May 2022

Plasma spray-physical vapor deposition (PS-PVD) as a novel process was used to prepare feather-like columnar thermal barrier coatings (TBCs). This special microstructure shows good strain tolerance and non-line-of-sight (NLOS) deposition, giving great potential application in aero-engine. However, due to serious service environment of aero-engine, particle erosion performance is a weakness for PS-PVD 7YSZ TBCs. As a solution, an Al-modification approach was proposed in this investigation. Through in-situ reaction of Al and ZrO2, an α-Al2O3 overlay can be formed on the surface of 7YSZ columnar coating. The results demonstrate that this approach can improve particle erosion resistance since hardness improvement of Al-modified TBCs. Meanwhile, as another important performance of thermal cycle, it has a better optimization with 350-cycle water-quenching, compared with the as-sprayed TBCs.
Isolation and characterization of novel plekhm1 and def8 mutant alleles in Drosophila
Authors: Maruzs Tamás , Lakatos Enikő , Feil-Börcsök Dalma , Lőrincz Péter , Juhász Gábor
Published: May 2022

Lysosomal degradation of cytoplasmic components by autophagy ensures the continuous turnover of proteins and organelles and aids cellular survival during nutrient deprivation and other stress conditions. Lysosomal targeting of cytoplasmic proteins and organelles requires the concerted action of several proteins and multisubunit complexes. The core components of this machinery are conserved from yeast to humans and many of them are well-characterized; however, novel molecular players have been recently discovered and are waiting for detailed analysis. The osteopetrosis-linked PLEKHM1 protein is a lysosomal adaptor involved in autophagosome and endosome to lysosome fusion events and its role in lysosomal positioning in osteoclasts was reported together with its proposed binding partner, the relatively uncharacterized DEF8 protein. Here, we report the generation and subsequent analysis of novel mutant alleles of Drosophila plekhm1 and def8. Interestingly, the CRISPR-generated null mutations of these genes do not have any obvious effects on autophagy in Drosophila tissues, even though RNAi knockdown of these genes seems to perturb autophagy. Although these results are quite surprising and raise the possibility of compensatory changes in the case of null mutants, the new alleles will be valuable tools in future studies to understand the cellular functions of Drosophila Plekhm1 and Def8 proteins.
A Procedural Justice Theory Approach to Police Engagement with Victim-Survivors of Rape and Sexual Assault: Initial Findings of the ‘Project Bluestone’ Pilot Study
Authors: Hohl Katrin , Johnson Kelly , Molisso Sarah
Published: May 2022

In England and Wales, public trust in the police has been damaged by a series of police failings in rape and sexual assault investigations, officer sexual offending, and a police culture of misogyny. Feminist scholars have analysed why police investigations of rape and sexual assault cases rarely result in a charge and documented the poor experiences many victim-survivors have of the police process. In this article, we outline how this scholarship may be integrated into procedural justice theory to advance our understanding of the impact of how officers engage with victim-survivors on their feelings of the status and value as survivors of sexual violence within the nation and society police represent, as well as on their trust in the police and willingness to (continue) engaging with police, or report future victimisation. We present tentative evidence from a pilot study (‘Project Bluestone’) in one English police force that suggests a feminist scholarship informed Procedural Justice framework is a promising tool for assessing and improving police practice in engaging with victim-survivors of rape and sexual assault. The article concludes with directions for future research.
Programming in Parallel with CUDA
Authors: Ansorge Richard
Published: May 2022

CUDA is now the dominant language used for programming GPUs, one of the most exciting hardware developments of recent decades. With CUDA, you can use a desktop PC for work that would have previously required a large cluster of PCs or access to a HPC facility. As a result, CUDA is increasingly important in scientific and technical computing across the whole STEM community, from medical physics and financial modelling to big data applications and beyond. This unique book on CUDA draws on the author's passion for and long experience of developing and using computers to acquire and analyse scientific data. The result is an innovative text featuring a much richer set of examples than found in any other comparable book on GPU computing. Much attention has been paid to the C++ coding style, which is compact, elegant and efficient. A code base of examples and supporting material is available online, which readers can build on for their own projects.
A Life of Sir Harry Parkes
Authors: Morton Robert
Published: May 2022

Harry Parkes was at the heart of Britain's relations with the Far East from the start of his working life at fourteen, to his death at fifty-seven. Orphaned at the age of five, he went to China on his own as a child and worked his way to the top. God-fearing and fearless, he believed his mission was to bring trade and 'civilisation' to East Asia. In his day, he was seen as both a hero and a monster and is still bitterly resented in China for his part in the country's humiliations at Western hands, but largely esteemed in Japan for helping it to industrialise. Morton's new biography, the first in over thirty years, and benefitting in part from access to the Parkes' family and archives, offers a more intimate and informed profile of the personal and professional life of a Victorian titan and one of Britain's most undiplomatic diplomats in the history of the British Civil Service.
Improvements in learning to control perched landings
Authors: Fletcher L. , Clarke R. , Richardson T. , Hansen M.
Published: May 2022

Reinforcement learning has previously been applied to the problem of controlling a perched landing manoeuvre for a custom sweep-wing aircraft. Previous work showed that the use of domain randomisation to train with atmospheric disturbances improved the real-world performance of the controllers, leading to increased reward. This paper builds on the previous project, investigating enhancements and modifications to the learning process to further improve performance, and reduce final state error. These changes include modifying the observation by adding information about the airspeed to the standard aircraft state vector, employing further domain randomisation of the simulator, optimising the underlying RL algorithm and network structure, and changing to a continuous action space. Simulated investigations identified hyperparameter optimisation as achieving the most significant increase in reward performance. Several test cases were explored to identify the best combination of enhancements. Flight testing was performed, comparing a baseline model against some of the best performing test cases from simulation. Generally, test cases that performed better than the baseline in simulation also performed better in the real world. However, flight tests also identified limitations with the current numerical model. For some models, the chosen policy performs well in simulation yet stalls prematurely in reality, a problem known as the reality gap.
On (non-)conservative body forces, vorticity generation and energy conversion in ideal flows
Authors: van Kuik G.A.M.
Published: May 2022

Usually, the load on lifting bodies in incompressible, inviscid flow is determined by integration of the pressure on the body surface, once the flow is solved. Prandtl (Nachrichten von der Gesellschaft der Wissenschaften zu Göttingen, Mathematisch- Physikalische Klasse, vol. 1918, pp. 451–477) proposed an opposite method in which the body force field is the source term in the equation of motion to solve the flow. This force field method has not been used intensively but has regained importance in modern wind energy research. However, an analysis of which type of body force field generates vorticity and converts energy, and which body force field does not, is lacking. Prandtl's method is adopted here, but with the addition that the force field is allowed to be conservative or non-conservative. The relation between conservative/non-conservative body forces, vorticity generation, potential and kinetic energy and Helmholtz's vorticity theorems are derived. Similarly, the load on lifting bodies in two and three dimensions is classified as (non-)conservative, with some examples. To show that the force field method is consistent with the method where the load is output of an analysis, the expression for the Kutta–Joukowsky load and the relation between bound and trailing vorticity of a wing have been rederived using the force field method. The analysis confirms that this relation between bound and trailing vorticity is not governed by Helmholtz's theorems, as is often assumed, but by the non-conservative force field generating vorticity.
Dynamics of Policy and Politics: Politics of Unemployment in Sweden during the Interwar Period
Authors: Ahn Jae-Hung
Published: May 2022

Inspired by the theoretical perspective that “new policies create a new politics,” this paper explores how unemployment policies affected the politics of tripartite relations in Sweden during the interwar period. After the economic depression of 1920, strike activities began to decrease. Our panel data analysis finds that after 1920, the strength of the relationship between unemployment and strike activities decreased substantially. Historical interpretations complement statistical analysis. In the 1920s, the implementation of unemployment policies entailed the following feedback effects: First, the state reinforced its capacities, gaining increasingly firm control over strike activities. Second, the union movement was plunged into internal conflicts. In contrast to the arguments of power resources theory and the theory of cross-class coalition, neither the empowerment of the Social Democratic Workers’ Party (SAP) nor employers’ lockouts induced the Confederation of Labor Unions (LO) to turn towards concertation. Rather, this turnaround occurred in the 1920s while LO coped with the feedback effects engendered endogenously in processes of the implementation of unemployment policies.
Arthrospira (Spirulina) platensis feeding reduces the early stage of chemically induced rat colon carcinogenesis
Authors: Amadeu Simone Oliveira , Sarmiento-Machado Luis Manuel , Bartolomeu Ariane Rocha , Chaves María Angel García , Romualdo Guilherme Ribeiro , de Moura Nelci Antunes , Barbisan Luis Fernando
Published: May 2022

Colorectal cancer is the third most diagnosed cancer worldwide and linked to dietary/lifestyle factors. Arthrospira (Spirulina) platensis (AP) contains bioactive compounds with beneficial effects in vivo/in vitro. We evaluated the effects of AP feeding against 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis. Male Sprague Dawley rats were given subcutaneous injections of DMH (4 × 40 mg/kg body weight) (G1–G3) or vehicle (G4–G5) twice a week (weeks 3–4). During weeks 1–4, animals were fed a diet containing 1 % (G2) or 2 % (G3–G4) AP powder (w/w). After this period, all groups received a balanced diet until week 12. Some animals were euthanised after the last DMH injection (week 4) for histological, immunohistochemical (Ki-67, γ-H2AX and caspase-3) and molecular analyses (real time-PCR for 91 genes), while other animals were euthanised at week 12 for preneoplastic aberrant crypt foci (ACF) analysis. Both AP treatments (G2–G3) significantly decreased the DMH-induced increase in γ-H2AX (DNA damage) and caspase 3 (DNA damage-induced cell death) in colonic crypts at week 4. In addition, Cyp2e1 (Drug metabolism), Notch1, Notch2 and Jag1 genes (Notch pathway) and Atm, Wee1, Chek2, Mgmt, Ogg1 and Xrcc6 genes (DNA repair) were also down-regulated by 2 % AP feeding (G3) at week 4. A significant reduction in ACF development was observed in both AP-treated groups (G2–G3) at week 12. In conclusion, findings indicate that AP feeding reduced acute colonic damage after DMH, resulting in fewer preneoplastic lesions. Our study provided mechanistic insights on dietary AP-preventive effects against early colon carcinogenesis.
Equity Trading Activity and Treasury Bond Risk Premia
Authors: Schraeder Stefanie , Sojli Elvira , Subrahmanyam Avanidhar , Tham Wing W.
Published: May 2022

We link equity and treasury bond markets via an informational channel. When macroeconomic state shifts are more probable, informed traders are more likely to have valid signals about fundamentals, so that uninformed traders are less willing to trade against informed ones. This implies low volume and high volatility, that is, a high volatility–volume ratio (VVR). Central banks react to state shifts, but their actions are uncertain. Therefore, a higher state shift likelihood implies larger bond risk premia. These arguments together imply that VVR should positively predict bond excess returns. We empirically test and confirm this prediction, both in- and out-of-sample.
Redescription of Diastolaimus grossus (Truskova & Eroshenko, 1977) Andrássy, 1984 (Rhabditida, Chambersiellidae) from Europe and comments on its phylogenetic position
Authors: Čermák V. , Nježić B. , Foit J. , Kulich P. , Orságová H. , Ruiz-Cuenca A.N. , Shokoohi E. , Abolafia J.
Published: May 2022

Two populations of the species Diastolaimus grossus have been obtained from bark of trees in Bosnia and Herzegovina and the Czech Republic. The species is described and characterized in detail using morphological techniques (light and electron scanning microscopy) and morphometrical (Gower General Similarity coefficient of morphological characters) and molecular analyses, including phylogenetic analysis of all related and already sequenced species of the family Chambersiellidae. Morphological and molecular analyses, based on 18S and 28S ribosomal DNA sequences, show that the family Chambersiellidae is polyphyletic, being the subfamily Chambersiellinae related with Cephalobomorpha and Tylenchomorpha, and the subfamily Macrolaiminae is located into Panagrolaimomorpha. The genus Diastolaimus, previously belonging to Macrolaiminae, is transferred to Chambersiellinae. Diastolaimus mexicanus is proposed as a junior synonym of D. grossus.
The effect of frequency of exposure on the processing and learning of collocations: A comparison of first and second language readers’ eye movements
Authors: Pellicer-Sánchez Ana , Siyanova-Chanturia Anna , Parente Fabio
Published: May 2022

This study examined the processing and acquisition of novel words and their collocates (i.e., words that frequently co-occur with other words) from reading and the effect of frequency of exposure on this process. First and second language speakers of English read a story with 1) eight exposures of adjective-pseudoword collocations, 2) four exposures of the same collocations, or 3) eight exposures of control collocations. Results of recall and recognition tests showed that participants acquired knowledge not only of the form and meaning of the pseudowords but also of their collocates. The analysis of eye movements showed a significant effect of exposure on the processing of novel collocations for both first and second language readers, with reading times decreasing as a function of exposure. Eight exposures to novel adjective-pseudoword collocations were enough to develop processing speed comparable to that of known collocations. However, when analyzing the processing of the individual components of the collocations, results showed that eight exposures to the pseudowords were not enough for second language readers to develop processing speed comparable to known words. The frequency manipulation in the present study (four vs. eight exposures) did not lead to differences in the learning or processing of collocations. Finally, reading times were not a significant predictor of vocabulary gains.
Socialist, Humanist and Well-Designed: The Polish Welfare State at the International Labour Exhibition in Turin, 1961
Authors: Jeżowska Katarzyna
Published: May 2022

This article examines the Polish exhibit at the International Labour Exhibition in Turin, 1961, which presented Poland as a socialist welfare state. By locating the display within the broader historical context of Cold War competitiveness, the article intends to make two points. Firstly, using written and visual sources, this article explores how the humanistic dimension of the socialist welfare project, which remained largely unmatched by the capitalist West, was visualised and rationalised. Effectively, it proposes reading of social benefits and state subsidised services as a novel subject of cultural diplomacy. Secondly, by indicating the role of designers as significant stakeholders who actively shaped the country's self-imagining abroad, this article advances the scholarship about design diplomacy. It evidences that modern design allowed the exhibition makers to convey both symbolic and material aspects of the welfare state, which had at stake the battle for hearts and minds.
How similar are shared syntactic representations? Evidence from priming of passives in Greek–English bilinguals
Authors: Kotzochampou Sotiria , Chondrogianni Vasiliki
Published: May 2022

The shared-syntax account of bilingual syntactic representations suggests that similar structures from different languages are represented as one in the bilingual mind. In this study, we examined the degree of morpho-syntactic similarity needed for representations to be shared in the bilingual mind by comparing passive structures in Greek and English. Contrary to English, non-active morphology in Greek is not restricted to passives and the “by phrase” is considered marked. In two structural priming experiments, we examined whether passives can be primed in L1-Greek and, subsequently, whether there is a single representation for passives in Greek–English bilinguals despite distributional and morpho-syntactic differences. Results showed that passive structures were primed in L1-Greek (Experiment 1) and from L1-Greek to L2-English (Experiment 2). Our findings suggest that morpho-syntactic and distributional differences inherent to passives do not prevent priming, and that structural representations can be shared even when featural structure is not identical.
Exploration, collection and characterization of Kala zeera (Bunium persicum Boiss. Fedtsch.) germplasm from northwestern Himalayas
Authors: Khan Mohd Anwar , Altaf Suhail , Shafi Safoora , Bhat Bilal Ahmad , Dar Waseem Ali , Parry Feroz Ahmad , Mir Reyazul Rouf
Published: May 2022

A study was conducted to collect and characterize the indigenous Kala zeera (Bunium persicum Boiss. Fedtsch.) accessions from the hills of northwestern Himalayan sates of Jammu and Kashmir, Himachal Pradesh and Uttarakhand. Around 1000 accessions were collected during this exploration mission, out of which diversity of 252 accessions was established through morphological characterization. The morpho-agronomic characterization and the analysis of trait data revealed significant variability in number of branches per plant, number of umbelets per umbel, number of seeds per plant, seed yield per plant and 1000 seed weight. The collection and characterization of these diverse accessions can prove useful in future Kala zeera improvement programmes in the world as this is the first such comprehensive report of the crop from northwestern Himalayan region of India.
ACTUAL AND INTELLECTUAL ARROWSHOTS IN SOPHRON (FRR. 50 AND 86 KASSEL–AUSTIN)
Authors: Cassio A. C.
Published: May 2022

After some notes on the remarkable linguistic difficulties encountered by ancient Athenian readers of Sophron's text, this article will deal with the textual and interpretative problems posed by two fragments of the mimes that are more significant than it may appear at first sight. It is argued that a verbal form transmitted in fr. 86 K–A, whose correctness has systematically been doubted, is in fact linguistically unassailable and used in the description of an ambush that might be either military or metaphorical. As to fr. 50, once the correct reading of the principal verb has been established, we recover a subtle metaphor used by a character in a mime to criticise those who make hasty speculations about things unknown –something that reveals a sophistication rarely encountered in other fragments, and provides us with a glimpse into the reasons why Sophron's mimes enjoyed such respect in antiquity.
Urbanization Contributes Little to Global Warming but Substantially Intensifies Local and Regional Land Surface Warming
Authors: Zhou Decheng , Xiao Jingfeng , Frolking Steve , Zhang Liangxia , Zhou Guoyi
Published: May 2022

Increasing urbanization causes an urban heat island (UHI) effect and exacerbates health risks of heat waves due to global warming. The surface UHI (SUHI) in large cities has been extensively studied, yet a systematic evaluation on the impacts of urbanization on local‐to global‐scale land surface warming is lacking. We propose a new procedure to quantify the warming effects of urbanization at local, regional, and global scales using high‐resolution satellite observations. We find strong local warming effects for 88% of the urban‐dominated pixels across the globe and cooling effects for the rest of the urban lands on a diurnal mean timescale, with a global urban mean intensity of 1.1°C in 2015. The SUHI effects differ substantially by time of day, season, and climate zone, and are closely related to surface evapotranspiration. By extending local effects to the entire land surface, we estimate a diurnal mean warming of only 0.008°C globally. However, urbanization can have large warming effects regionally, especially in eastern China, the eastern United States, and Europe. In addition, we show that global urban expansion results in over three‐quarters of SUHI effects in 1985–2015, and its effect will likely increase by 50%–200% by the end of this century. The SUHI‐added warming could be up to 0.12°C in summer in Europe by 2100 under a fossil‐fueled development pathway. Our results reveal that urbanization substantially intensifies local and regional land surface warming and that prioritized attention should be given to the SUHI‐added warming in highly or rapidly urbanized regions.
Earthquake Modelers Unite to Compare and Improve Code
Authors: Stanley Sarah
Published: May 2022

International community–driven efforts lend confidence to fault-slip simulations while highlighting key discrepancies.
Fundamental Science and Engineering Questions in Planetary Cave Exploration
Authors: Wynne J. Judson , Titus Timothy N. , Agha‐Mohammadi Ali‐akbar , Azua‐Bustos Armando , Boston Penelope J. , de León Pablo , Demirel‐Floyd Cansu , De Waele Jo , Jones Heather , Malaska Michael J. , Miller Ana Z. , Sapers Haley M. , Sauro Francesco , Sonderegger Derek L. , Uckert Kyle , Wong Uland Y. , Alexander E. Calvin , Chiao Leroy , Cushing Glen E. , DeDecker John , Fairén Alberto G. , Frumkin Amos , Harris Gary L. , Kearney Michelle L. , Kerber Laura , Léveillé Richard J. , Manyapu Kavya , Massironi Matteo , Mylroie John E. , Onac Bogdan P. , Parazynski Scott E. , Phillips‐Lander Charity M. , Prettyman Thomas H. , Schulze‐Makuch Dirk , Wagner Robert V. , Whittaker William L. , Williams Kaj E.
Published: May 2022

Nearly half a century ago, two papers postulated the likelihood of lunar lava tube caves using mathematical models. Today, armed with an array of orbiting and fly‐by satellites and survey instrumentation, we have now acquired cave data across our solar system—including the identification of potential cave entrances on the Moon, Mars, and at least nine other planetary bodies. These discoveries gave rise to the study of planetary caves. To help advance this field, we leveraged the expertise of an interdisciplinary group to identify a strategy to explore caves beyond Earth. Focusing primarily on astrobiology, the cave environment, geology, robotics, instrumentation, and human exploration, our goal was to produce a framework to guide this subdiscipline through at least the next decade. To do this, we first assembled a list of 198 science and engineering questions. Then, through a series of social surveys, 114 scientists and engineers winnowed down the list to the top 53 highest priority questions. This exercise resulted in identifying emerging and crucial research areas that require robust development to ultimately support a robotic mission to a planetary cave—principally the Moon and/or Mars. With the necessary financial investment and institutional support, the research and technological development required to achieve these necessary advancements over the next decade are attainable. Subsequently, we will be positioned to robotically examine lunar caves and search for evidence of life within Martian caves; in turn, this will set the stage for human exploration and potential habitation of both the lunar and Martian subsurface.
Reappraising the Production and Transfer of Hydrogen Atoms From the Middle to the Upper Atmosphere of Mars at Times of Elevated Water Vapor
Authors: Montmessin F. , Belyaev D. A. , Lefèvre F. , Alday J. , Vals M. , Fedorova A. A. , Korablev O. I. , Trokhimovskiy A. V. , Chaffin M. S. , Schneider N. M.
Published: May 2022

Water escape on Mars has recently undergone a paradigm shift with the discovery of unexpected seasonal variations in the population of hydrogen atoms in the exosphere where thermal escape occurs and results in water lost to space. This discovery led to the hypothesis that, contradicting the accepted pathway, atomic hydrogen in the exosphere was not only produced by molecular hydrogen but mostly by high altitude water vapor. Enhanced presence of water at high altitude during southern spring and summer, due to atmospheric warming and intensified transport, favors production of H through photon‐induced ion chemistry of water molecules and thus appears to be the main cause of the observed seasonal variability in escaping hydrogen. This hypothesis is supported by the observation of large concentrations of water vapor between 50 and 150 km during the southern summer solstice and global dust events. Using a simplified yet representative air parcel transport model, we show that in addition to the formation of atomic hydrogen from water photolysis above 80 km, a major fraction of the exospheric hydrogen is formed at altitudes as low as 60 km and is then directly advected to the upper atmosphere. Comparing the injection modes of a variety of events (global dust storm, perihelion periods, and regional storm), we conclude that southern spring/summer controls H production and further ascent into the upper atmosphere on the long term with direct implication for water escape.
Color-preserving passive radiative cooling for an actively temperature-regulated enclosure
Authors: Zhu Yining , Luo Hao , Yang Chenying , Qin Bing , Ghosh Pintu , Kaur Sandeep , Shen Weidong , Qiu Min , Belov Pavel , Li Qiang
Published: May 2022

Active temperature control devices are widely used for the thermal management of enclosures, including vehicles and buildings. Passive radiative cooling has been extensively studied; however, its integration with existing actively temperature regulated and decorative enclosures has slipped out of the research at status quo. Here, we present a photonic-engineered dual-side thermal management strategy for reducing the active power consumption of the existing temperature-regulated enclosure without sacrificing its aesthetics. By coating the exterior and interior of the enclosure roof with two visible-transparent films with distinctive wavelength-selectivity, simultaneous control over the energy exchange among the enclosure with the hot sun, the cold outer space, the atmosphere, and the active cooler can be implemented. A power-saving of up to 63% for active coolers of the enclosure is experimentally demonstrated by measuring the heat flux compared to the ordinary enclosure when the set temperature is around 26°C. This photonic-engineered dual-side thermal management strategy offers facile integration with the existing enclosures and represents a new paradigm toward carbon neutrality.
The effects of probiotics plus dietary fiber on antipsychotic-induced weight gain: a randomized clinical trial
Authors: Huang Jing , Liu Chenchen , Yang Ye , Kang Dongyu , Xiao Jingmei , Long Yujun , Lang Bing , Peng Xingjie , Wang Weiyan , Wang Xiaoyi , Liu Fangkun , Zhao Jingping , Shi Zhe , Yuan Ti-Fei , Wu Renrong
Published: May 2022

Probiotics plus dietary fiber has demonstrated efficacy in improving metabolic abnormalities. However, the efficacy of probiotics and dietary fiber as well as their association with microbiota in attenuating antipsychotic-induced weight gain and metabolic disturbance remains poorly understood. Here we analyzed results from the double-blind, randomized, placebo-controlled study to compare and evaluate the effects of probiotics, dietary fiber, and their combination for antipsychotic-induced weight gain in patients with a severe mental disorder. We found that probiotics plus dietary fiber was significantly superior to probiotics alone, dietary fiber only, and the placebo for weight, BMI, and total cholesterol reduction; insulin resistance was worse in the placebo group, with significant increases during the 12-week treatment; probiotics plus dietary fiber significantly reduced weight and prevented further deterioration of metabolic disturbances; and probiotics or dietary fiber alone can prevent further weight gain. We further performed 16 S ribosomal RNA sequencing revealed an increased abundance of microbiota after probiotics plus dietary fiber treatment. Moreover, logistic regression analyses revealed that the higher richness of microbiota was associated with favorable weight loss. These findings suggested that probiotics and dietary fiber co-administration were safe and effective interventions to reduce weight gain in patients treated with antipsychotic medications.
Nonlinear mechanics of human mitotic chromosomes
Authors: Meijering Anna E. C. , Sarlós Kata , Nielsen Christian F. , Witt Hannes , Harju Janni , Kerklingh Emma , Haasnoot Guus H. , Bizard Anna H. , Heller Iddo , Broedersz Chase P. , Liu Ying , Peterman Erwin J. G. , Hickson Ian D. , Wuite Gijs J. L.
Published: May 2022

In preparation for mitotic cell division, the nuclear DNA of human cells is compacted into individualized, X-shaped chromosomes1. This metamorphosis is driven mainly by the combined action of condensins and topoisomerase IIα (TOP2A)2,3, and has been observed using microscopy for over a century. Nevertheless, very little is known about the structural organization of a mitotic chromosome. Here we introduce a workflow to interrogate the organization of human chromosomes based on optical trapping and manipulation. This allows high-resolution force measurements and fluorescence visualization of native metaphase chromosomes to be conducted under tightly controlled experimental conditions. We have used this method to extensively characterize chromosome mechanics and structure. Notably, we find that under increasing mechanical load, chromosomes exhibit nonlinear stiffening behaviour, distinct from that predicted by classical polymer models4. To explain this anomalous stiffening, we introduce a hierarchical worm-like chain model that describes the chromosome as a heterogeneous assembly of nonlinear worm-like chains. Moreover, through inducible degradation of TOP2A5specifically in mitosis, we provide evidence that TOP2A has a role in the preservation of chromosome compaction. The methods described here open the door to a wide array of investigations into the structure and dynamics of both normal and disease-associated chromosomes.
3D Reconstruction of cellular images from microfabricated imagers using fully-adaptive deep neural networks
Authors: Najafiaghdam Hossein , Rabbani Rozhan , Gharia Asmaysinh , Papageorgiou Efthymios P. , Anwar Mekhail
Published: May 2022

Millimeter-scale multi-cellular level imagers enable various applications, ranging from intraoperative surgical navigation to implantable sensors. However, the tradeoffs for miniaturization compromise resolution, making extracting 3D cell locations challenging—critical for tumor margin assessment and therapy monitoring. This work presents three machine-learning-based modules that extract spatial information from single image acquisitions using custom-made millimeter-scale imagers. The neural networks were trained on synthetically-generated (using Perlin noise) cell images. The first network is a convolutional neural network estimating the depth of a single layer of cells, the second is a deblurring module correcting for the point spread function (PSF). The final module extracts spatial information from a single image acquisition of a 3D specimen and reconstructs cross-sections, by providing a layered “map” of cell locations. The maximum depth error of the first module is 100 µm, with 87% test accuracy. The second module’s PSF correction achieves a least-square-error of only 4%. The third module generates a binary “cell” or “no cell” per-pixel labeling with an accuracy ranging from 89% to 85%. This work demonstrates the synergy between ultra-small silicon-based imagers that enable in vivo imaging but face a trade-off in spatial resolution, and the processing power of neural networks to achieve enhancements beyond conventional linear optimization techniques.
Diagnostic performance of a 5-plex malaria immunoassay in regions co-endemic for Plasmodium falciparum, P. vivax, P. knowlesi, P. malariae and P. ovale
Authors: Kho Steven , Anstey Nicholas M. , Barber Bridget E. , Piera Kim , William Timothy , Kenangalem Enny , McCarthy James S. , Jang Ihn Kyung , Domingo Gonzalo J. , Britton Sumudu , Grigg Matthew J.
Published: May 2022

Commercial point-of-care tests remain insufficient for accurately detecting and differentiating low-level malaria infections in regions co-endemic with multiple non-falciparum species, including zoonotic Plasmodium knowlesi (Pk). A 5-plex chemiluminescent assay simultaneously measures pan-Plasmodium lactate dehydrogenase (pLDH), P. falciparum (Pf)-LDH, P. vivax (Pv)-LDH, Pf-histidine-rich protein-2 (HRP2), and C-reactive protein. We assessed its diagnostic performance on whole blood (WB) samples from 102 healthy controls and 306 PCR-confirmed clinical cases of Pf, Pv, Pk, P. malariae (Pm) and P. ovale (Po) mono-infections from Southeast-Asia. We confirm its excellent HRP2-based detection of Pf. Cross-reactivity of Pf-LDH with all non-falciparum species tested was observed (specificity 57.3%). Pv-LDH performance was suboptimal for Pv (93.9% sensitivity and 73.9% specificity). Poor specificity was driven by strong Pk cross-reactivity, with Pv-LDH detecting 93.9% of Pk infections. The pan-LDH-to-Pf-LDH ratio was capable of discerning Pv from Pk, and robustly differentiated Pf from Pm or Po infection, useful in regions with hrp2/3 deletions. We tested the platform’s performance in plasma for the first time, with WB outperforming plasma for all analytes except Pv-LDH for Pk. The platform is a promising tool for WB malaria diagnosis, although further development is warranted to improve its utility in regions co-endemic for multiple non-falciparum species.
Autistic-like behavioral effects of prenatal stress in juvenile Fmr1 mice: the relevance of sex differences and gene–environment interactions
Authors: Petroni Valeria , Subashi Enejda , Premoli Marika , Wöhr Markus , Crusio Wim E. , Lemaire Valerie , Pietropaolo Susanna
Published: May 2022

Fragile X Syndrome (FXS) is the most common heritable form of mental retardation and monogenic cause of autism spectrum disorder (ASD). FXS is due to a mutation in the X-linked FMR1 gene and is characterized by motor, cognitive and social alterations, mostly overlapping with ASD behavioral phenotypes. The severity of these symptoms and their timing may be exacerbated and/or advanced by environmental adversity interacting with the genetic mutation. We therefore tested the effects of the prenatal exposure to unpredictable chronic stress on the behavioral phenotype of juveniles of both sexes in the Fmr1 knock-out (KO) mouse model of FXS. Mice underwent behavioral tests at 7–8 weeks of age, that is, when most of the relevant behavioral alterations are absent or mild in Fmr1-KOs. Stress induced the early appearance of deficits in spontaneous alternation in KO male mice, without exacerbating the behavioral phenotype of mutant females. In males stress also altered social interaction and communication, but mostly in WT mice, while in females it induced effects on locomotion and communication in mice of both genotypes. Our data therefore highlight the sex-dependent relevance of early environmental stressors to interact with genetic factors to influence the appearance of selected FXS- and ASD-like phenotypes.
Effects of phosphorus-modified biochar as a soil amendment on the growth and quality of Pseudostellaria heterophylla
Authors: Ng Charles Wang Wai , Wang Yu Chen , Ni Jun Jun , So Pui San
Published: May 2022

Phosphorus (P) deficiency in agricultural soil is a worldwide concern. P modification of biochar, a common soil conditioner produced by pyrolysis of wastes and residues, can increase P availability and improve soil quality. This study aims to investigate the effects of P-modified biochar as a soil amendment on the growth and quality of a medicinal plant (Pseudostellaria heterophylla). P. heterophylla were grown for 4 months in lateritic soil amended with P-modified and unmodified biochar (peanut shell) at dosages of 0, 3% and 5% (by mass). Compared with unmodified biochar, P-modified biochar reduced available heavy metal Cd in soil by up to 73.0% and osmotic suction in the root zone by up to 49.3%. P-modified biochar application at 3% and 5% promoted the tuber yield of P. heterophylla significantly by 68.6% and 136.0% respectively. This was different from that in unmodified biochar treatment, where tuber yield was stimulated at 3% dosage but inhibited at 5% dosage. The concentrations of active ingredients (i.e., polysaccharides, saponins) in tuber were increased by 2.9–78.8% under P-modified biochar amendment compared with control, indicating the better tuber quality. This study recommended the application of 5% P-modified biochar for promoting the yield and quality of P. heterophylla.
Cordyceps inhibits ceramide biosynthesis and improves insulin resistance and hepatic steatosis
Authors: Li Ying , Talbot Chad Lamar , Chandravanshi Bhawna , Ksiazek Alec , Sood Ayushi , Chowdhury Kamrul Hasan , Maschek J. Alan , Cox James , Babu Adhini Kuppuswamy Satheesh , Paz Henry A. , Babu Pon Velayutham Anandh , Meyerholz David K. , Wankhade Umesh D. , Holland William , Shyong Tai E. , Summers Scott A. , Chaurasia Bhagirath
Published: May 2022

Ectopic ceramide accumulation in insulin-responsive tissues contributes to the development of obesity and impairs insulin sensitivity. Moreover, pharmacological inhibition of serine palmitoyl transferase (SPT), the first enzyme essential for ceramide biosynthesis using myriocin in rodents reduces body weight and improves insulin sensitivity and associated metabolic indices. Myriocin was originally extracted from fruiting bodies of the fungus Isaria sinclairii and has been found abundant in a number of closely related fungal species such as the Cordyceps. Myriocin is not approved for human use but extracts from Cordyceps are routinely consumed as part of traditional Chinese medication for the treatment of numerous diseases including diabetes. Herein, we screened commercially available extracts of Cordyceps currently being consumed by humans, to identify Cordyceps containing myriocin and test the efficacy of Cordyceps extract containing myriocin in obese mice to improve energy and glucose homeostasis. We demonstrate that commercially available Cordyceps contain variable amounts of myriocin and treatment of mice with a human equivalent dose of Cordyceps extract containing myriocin, reduces ceramide accrual, increases energy expenditure, prevents diet-induced obesity, improves glucose homeostasis and resolves hepatic steatosis. Mechanistically, these beneficial effects were due to increased adipose tissue browning/beiging, improved brown adipose tissue function and hepatic insulin sensitivity as well as alterations in the abundance of gut microbes such as Clostridium and Bilophila. Collectively, our data provide proof-of-principle that myriocin containing Cordyceps extract inhibit ceramide biosynthesis and attenuate metabolic impairments associated with obesity. Moreover, these studies identify commercially available Cordyceps as a readily available supplement to treat obesity and associated metabolic diseases.
Metformin combined with local irradiation provokes abscopal effects in a murine rectal cancer model
Authors: Tojo Mineyuki , Miyato Hideyo , Koinuma Koji , Horie Hisanaga , Tsukui Hidenori , Kimura Yuki , Kaneko Yuki , Ohzawa Hideyuki , Yamaguchi Hironori , Yoshimura Kotaro , Lefor Alan Kawarai , Sata Naohiro , Kitayama Joji
Published: May 2022

Although preoperative chemoradiation therapy can down-stage locally advanced rectal cancer (LARC), it has little effect on distant metastases. Metformin exerts an anti-cancer effect partly through the activation of host immunity. LuM1, a highly lung metastatic subclone of colon 26, was injected subcutaneously (sc) in BALB/c mice and treated with metformin and/or local radiation (RT). Lung metastases and the primary tumors were evaluated and the phenotypes of immune cells in the spleen and lung metastases were examined with flow cytometry and immunohistochemistry. Local RT, but not metformin, partially delayed the growth of sc tumor which was augmented with metformin. Lung metastases were unchanged in metformin or RT alone, but significantly reduced in the combined therapy. The ratios of splenic T cells tended to be low in the RT group, which were increased by the addition of metformin. IFN-γ production of the splenic CD4(+) and CD8(+) T cells was enhanced and CD49b (+) CD335(+) activated NK cells was increased after combined treatment group. Density of NK cells infiltrating in lung metastases was increased after combination treatment. Metformin effectively enhances local and abscopal effects of RT though the activation of cell-mediated immunity and might be clinically useful for LARC.
Concurrent outcomes from multiple approaches of epistasis analysis for human body mass index associated loci provide insights into obesity biology
Authors: D’Silva Sheldon , Chakraborty Shreya , Kahali Bratati
Published: May 2022

Genome wide association studies (GWAS) have focused on elucidating the genetic architecture of complex traits by assessing single variant effects in additive genetic models, albeit explaining a fraction of the trait heritability. Epistasis has recently emerged as one of the intrinsic mechanisms that could explain part of this missing heritability. We conducted epistasis analysis for genome-wide body mass index (BMI) associated SNPs in Alzheimer’s Disease Neuroimaging Initiative (ADNI) and followed up top significant interacting SNPs for replication in the UK Biobank imputed genotype dataset. We report two pairwise epistatic interactions, between rs2177596 (RHBDD1) and rs17759796 (MAPK1), rs1121980 (FTO) and rs6567160 (MC4R), obtained from a consensus of nine different epistatic approaches. Gene interaction maps and tissue expression profiles constructed for these interacting loci highlights co-expression, co-localisation, physical interaction, genetic interaction, and shared pathways emphasising the neuronal influence in obesity and implicating concerted expression of associated genes in liver, pancreas, and adipose tissues insinuating to metabolic abnormalities characterized by obesity. Detecting epistasis could thus be a promising approach to understand the effect of simultaneously interacting multiple genetic loci in disease aetiology, beyond single locus effects.
A catalogue with semantic annotations makes multilabel datasets FAIR
Authors: Kostovska Ana , Bogatinovski Jasmin , Džeroski Sašo , Kocev Dragi , Panov Panče
Published: May 2022

Multilabel classification (MLC) is a machine learning task where the goal is to learn to label an example with multiple labels simultaneously. It receives increasing interest from the machine learning community, as evidenced by the increasing number of papers and methods that appear in the literature. Hence, ensuring proper, correct, robust, and trustworthy benchmarking is of utmost importance for the further development of the field. We believe that this can be achieved by adhering to the recently emerged data management standards, such as the FAIR (Findable, Accessible, Interoperable, and Reusable) and TRUST (Transparency, Responsibility, User focus, Sustainability, and Technology) principles. We introduce an ontology-based online catalogue of MLC datasets originating from various application domains following these principles. The catalogue extensively describes many MLC datasets with comprehensible meta-features, MLC-specific semantic descriptions, and different data provenance information. The MLC data catalogue is available at: http://semantichub.ijs.si/MLCdatasets.
Factors influencing degradation kinetics of mRNAs and half-lives of microRNAs, circRNAs, lncRNAs in blood in vitro using quantitative PCR
Authors: Wang Chong , Liu Hui
Published: May 2022

RNAs are rapidly degraded in samples and during collection, processing and testing. In this study, we used the same method to explore the half-lives of different RNAs and the influencing factors, and compared the degradation kinetics and characteristics of different RNAs in whole blood and experimental samples. Fresh anticoagulant blood samples were incubated at room temperature for different durations, RNAs were extracted, and genes, including internal references, were amplified by real-time quantitative PCR. A linear half-life model was established according to cycle threshold (Ct) values. The effects of experimental operations on RNA degradation before and after RNA extraction were explored. Quantitative analysis of mRNA degradation in samples and during experimental processes were explored using an orthogonal experimental design. The storage duration of blood samples at room temperature had the greatest influence on RNA degradation. The half-lives of messenger RNAs (mRNAs) was 16.4 h. The half-lives of circular RNAs (circRNAs), long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) were 24.56 ± 5.2 h, 17.46 ± 3.0 h and 16.42 ± 4.2 h, respectively. RNA degradation occurred mainly in blood samples. The half-life of mRNAs was the shortest among the four kinds of RNAs. Quantitative experiments related to mRNAs should be completed within 2 h. The half-lives of circRNAs and lncRNAs were longer than those of the former two.