bluuebunny/crossref_metadata_2025 huggingface dataset:
Appendix for Spleen SOP v1
Authors: Jorgensen Marda , Nick Jerelyn
Published: February 2020

This is an appendix diagram for spleen tissue cutting.
Probing the Underlying Structure of Modern Expectancy-Value Theory in Multicultural Education: A Bayesian Exploratory Factor Analysis
Authors: Yang Yan , Mindrila Diana
Published: February 2020

Expectancy-Value (EV) theory has been widely used in a plethora of domains except for multicultural education, a distinct and critical field in many countries due to increasing student diversity. In light of the domain-specific nature of the EV theory and the discrepancy between the theoretical framework and empirical models found in previous studies, the purpose of the present study was to explore the factors of the EV theory in multicultural education. Participants were 187 college students who completed the Multicultural Expectancy-Value Scale (EVS). Exploratory factor analysis (EFA) with Bayes estimation and GEOMIN rotation resulted in two factors: Value and Expectancy. The two factors had a positive significant correlation of .42, p<.001. Participants with a Master’s or Doctoral degree had significantly higher Expectancy beliefs in multicultural education than those with a Bachelor’s degree (t(47.727)=-2.90, p<.01). Although our finding was consistent with the major tenets of the theory that expectancy and value beliefs are two primary motivating factors, it did not fully support the theoretical model, indicating a more parsimonious factor structure may be more appropriate. The distinct factor model in our study suggests a need for further research in examining the structural validity of the EV theory in multicultural education
Social Competencies and Expectations Regarding the Impending Transition to Secondary School
Authors: Röder Mandy , Müller Anna Rebecca
Published: February 2020

Changing from elementary to secondary school is a life event that every child has to pass. Previous research has shown that some children feel more threatened by the transition than others. In the present study children’s expectations concerning the impending transition to secondary school were analyzed and it was investigated how individual levels of social competencies, aggressive behavior, peer-acceptance and victimization experiences are related to the expectations regarding the transition to secondary school. The results demonstrated that empathy and aggression are strongly related to the perception of the impending transition as challenge whereas victimization and peer-acceptance were related to the perception of the transition as threat. Furthermore, with the help of a cluster analysis, four groups of children showing different patterns of expectations were identified. The findings give valuable information about children’s perceptions of the impending school transition. It is discussed how teachers could support children to cope better with transition processes.
Investigating Preservice Mathematics Teachers’ Pedagogical Content Knowledge through Microteaching
Authors: Kula Ünver Semiha , Özgür Zekiye , Bukova Güzel Esra
Published: February 2020

The purpose of this study was to investigate how pre-service mathematics teachers’ pedagogical content knowledge was reflected in their microteaching. Twenty preservice secondary mathematics teachers participated in the study. The participants formed groups of two, three or four people of their choice, resulting in six groups in total. Each group designed and conducted a microteaching on a topic of their choice. The videotapes of the groups’ microteaching and their lesson plans constituted the data set for the study. We analyzed the data per the components of the pedagogical content knowledge framework outlined by various researchers. We found that the preservice mathematics teachers in general were knowledgeable about different instructional strategies and the curriculum about the topic of their microteaching, but their knowledge of learners was relatively poor. Implications for teacher education are discussed.
List of reviewers
Authors: Diez Palomar Javier
Published: February 2020

This is the list of reviewers in 2019.
Orexins and the brain reinforcing systems
Authors: Tissen Ilja Yu. , Lebedev Andrei A. , Bychkov Eugenii R. , Lavrov Nikanor V. , Morozov Vitalii I. , Shabanov Petr D.
Published: February 2020

The purpose of the review was to analyze the neurochemical and neurophysiological mechanisms of the orexin system and the role of orexin in body functions and behavior. The focus is on the participation of orexin in the mechanisms of reinforcement and the formation of addictive behavior. At the beginning of the review a history of the first works on the field of orexin and its receptors are described. Hypothalamic orexin-producing cells and effects on brain functions are described. The effects of orexins and their receptors on eating behavior, wakefulness and positive reinforcement are described. Then, the effects of orexins on narcolepsy was analyzed. The participation of orexins in the mechanisms of reinforcement and dependence and the key role of orexins in the development of addictive behaviors is described. The special role of orexin in the formation of ethanol dependence is shown. Next, the issue of the action of orexins under stress was considered. Orexins have been shown to play an important role in the regulation of the nervous and humoral mechanisms mediating the formation of emotional memory associated with negative experience. It is concluded that orexin can modulate the estimation of stress and probability for reaching stimulus. In this regard, orexin antagonists can be considered as possible promising means of preventing and treating disorders of the anxiety-phobic spectrum, as well as disorders associated with the use of addictive drugs caused by stress and environmental stimuli.
Dijital Ortamda Kurum İçi İletişim: İşletmelere Yönelik Bir Araştırma
Authors: BALTA PELTEKOĞLU Filiz , DEMİR ASKEROĞLU Emel
Published: February 2020

Kurumsal verimlilik, kurumsal bağlılık, kurumsal imaj ve itibar gibi nedenlerle işletmeler açısından bir gereklilik olan kurum içi iletişim, çalışanlar açısından da bir haktır. Bu nedenle çağdaş yönetim yaklaşımıyla işletmeler sadece dış hedef kitleleriyle değil, aynı zamanda iç hedef kitleleriyle de etkili iletişimi gerçekleştirmek zorundadır. Her iletişim aracının en kısa zamanda Halkla İlişkiler araç ve yöntemi haline geldiği gerçeği, dijital iletişim teknolojilerinin de işletmelerin Halkla İlişkiler stratejilerine dahil olmasının bir nedenidir. Ancak, işletmelerin yapısı ile yararlanılan halkla ilişkiler araç ve yöntemleri arasında bir ilişkiden söz edilebilir. Bu çalışmada, Çerkezköy’de faaliyet göstermekte olan farklı büyüklüklerdeki 70 işletmenin insan kaynakları birimiyle bir saha araştırması gerçekleştirilmiştir. Çalışmanın amacı, iletişim teknolojilerinde yaşanan değişimin kurum içi iletişime etkisi ile dijital iletişimin kurum içi iletişimde kullanım yaygınlığını ortaya çıkararak işletmelerin büyüklüğü ile kullanılan iletişim aracı/yöntemi arasındaki ilişkiyi anlamaya yöneliktir. Çalışmada nitel ve nicel araştırma yöntemleri kullanılmıştır. Elde edilen bulgular ışığında işletmelerin kurum içi iletişim uygulamalarında dijital iletişimi kullandığı, sosyal medya yöntemlerinin ise işletmelerin büyüklüklerine göre farklılıklar gösterdiği görülmüştür.
Proceso de embrionamiento y eclosión de Ascaris suum
Authors: Polo Gamboa Jaime Abelardo , Alva Sevilla Juan Miguel
Published: February 2020

Se presenta la siguiente secuencia fotográfica del proceso de embrionamiento de A. suum, la cual se llevó a cabo luego de la obtención de hembras adultas extraídas de los intestinos de cerdos infectados sacrificados en un matadero local de la ciudad de Trujillo, La Libertad, Perú. Los huevos al ser extraídos del útero de las hembras fueron decorticados con hidróxido de sodio al 0,1 N por 15 minutos para luego ser lavados con solución salina fisiológica y luego colocados en viales con agua destilada estéril a 25° C, para seguir la secuencia de embrionamiento. Las siguientes imágenes fueron tomadas en el Laboratorio de Microbiología y Parasitología de la Facultad de Ciencias de la Salud de la Universidad Cesar Vallejo, usando el microscopio Leica ICC50 con cámara integrada.
TEACHERS’ FAMILIARITY AND PRACTICES OF DYSLEXIA ASSOCIATED WITH WEAK WORKING MEMORY IN SAUDI ARABIA
Authors: Alqarni Turki
Published: February 2020

Purpose of the Study: The present study investigates teachers’ familiarity and practices of dyslexia related to weak working memory.

Methodology: A quantitative approach was used to collect data from teachers of learning disabilities. This paper presents the findings of a survey design research by a set of questionnaires carried out in southern regions of Saudi Arabia (n=90)out of 114 male teachers and 99 female teachers.

Results: The main findings indicated that teachers are familiar with information and practices related to dyslexia associated with poor working memory, and there are a significant prediction and relationship between the teachers’ familiarity and practices of this disorder.

Applications of this study: This study provides beneficial ideas and insights to reform the special education systems in Saudi Arabia regarding dyslexia associated with weak working memory. The results emphasized on increasing the familiarity of teachers to support their practices. This can be applied through the teacher's training, the strength of multidisciplinary teams, and dyslexia awareness.

Novelty/Originality of this study: The literature showed that there is an association between dyslexia and weak working memory. However, there is no study examined teachers' familiarity and practices of this association. This study attempt to fill in the gap by identifying the best variable predicting the teachers' familiarity and the practices of dyslexia associated with weak working memory.
Evolución y desarrollo legislativo del cooperativismo en Colombia desde 1991
Authors: Ángel Luz Karime , Barragán Ronderos Daniel Alfonso
Published: February 2020

El artículo tiene como objetivo principal, identificar el proceso legislativo del cooperativismo en Colombia, desde la promulgación de la Constitución de 1991 hasta el año 2016, describiendo fracciones de tiempo que abren un camino para el entendimiento de las actividades económicas y sociales legisladas sobre el cooperativismo en el país. El desarrollo del estudio y cumplimiento del objetivo general, está sujeto a una metodología netamente de enfoque cualitativo, que prevé el uso de una técnica de recolección de datos, en esta caso solo leyes, y su descripción.
La clasificación de los datos, conlleva a la identificación de tres periodos temporales históricos de producción legislativa sobre cooperativismo; un primer periodo de 1991 a 2004, donde se marca un nuevo inicio del cooperativismo a partir del nacimiento de la Constitución colombiana de 1991 y su desarrollo; un segundo periodo de 2005 a 2016, todo lo anterior está dirigido a construir un artículo de revisión, que construirá las bases del desarrollo de toda la investigación a la cual pertenece en el presente estudio.
La Amazonía colombiana como sujeto de derechos: caracterización del conflicto ambiental que llevó a su reconocimiento
Authors: Vargas-Chaves Iván , Luna-Galván Mauricio , Torres-Pérez Katy
Published: February 2020

A través de la aplicación de un instrumento metodológico de caracterización de conflictos ambientales, en el presente artículo se lleva a cabo un ejercicio descriptivo de los antecedentes del conflicto que llevó al reconocimiento de la Amazonía colombiana como sujeto de derechos por parte de la Corte Suprema de Justicia. Adicional a lo anterior, y con el propósito de estructurar este estudio de caso, se relacionan las posturas de los actores principales y secundarios, además de los aspectos sociales, económicos y ambientales identificados en la acción de tutela mediante la aplicación de un instrumento de caracterización de conflictos, en el que se examinan los principales actores y  factores sociales, gubernamentales, económicos y geográficos que desencadenaron la problemática ambiental y que permiten desde diferentes ópticas su comprensión. Por último, se presentan a manera de conclusiones las consideraciones que desde el biocentrismo le permitieron a la Corte fundamentar esta novedosa visión.
LASER SURGERY FOR CUTANEOUS SIMPLE CAPILLARY MALFORMATIONS IN CHILDREN
Authors: Shakina L. D. , Ponomarev I. V. , Fisenko A. P. , Smirnov I. E.
Published: February 2020

There are presented data on the epidemiology, pathogenesis, and diagnosis of cutaneous simple capillary malformations, described as Nevus simplex and Nevus flammeus (NF) in the Classification of Vascular Anomalies in a revision on May 2108. There are compared the efficacy and side effects of an application of laser irradiation of different wavelengths for the laser surgical treatment of NF in patients of various ages. There are described features of morphometry and angioarchitecture of the vascular bed of NF at the early and advanced stages of the disease. In order to substantiate the choice of the preferred laser irradiation settings, the peculiarities of absorption of the laser irradiation with different wavelengths by main cutaneous photothermophores (chromophores) are compared in children of various ages. For the first time, there are presented data on the age-related features of the absorption coefficient and the relevant absorption index for laser irradiation of different wavelengths. The maximum values of the absorption coefficients and the index of the relevance of the absorption of laser radiation with a wavelength of 578 nm by the main skin photothermophores indicate the greatest efficiency of the domestic copper vapor laser for the surgical treatment of NF in children of different ages.
Correlation between the Bacteriostatic and Bactericide Effect with Antibiofilm and Anticolony Spreading from Javanese Citronella Oil on Methicillin-Resistant Staphylococcus aureus (MRSA)
Authors: Hidayah Amaliyah Nurul , Wasito Eddy Bagus , Debora Kartuti , Basori Achmad , Isnaeni Isnaeni , Utomo Budi
Published: February 2020

Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogenic bacterium that has been resistant to various types of antibiotics, so it is not easy to be treated with antibiotics and needs other solutions. Javanese citronella oil distilled from the Cymbopogon nardus plant is proven to function as an antibacterial agent (bacteriostatic and bactericidal), fungicide and repellent. This study aimed to prove that there is a positive correlation between bacteriostatic and bactericidal effects with antibiofilm and anticolony spreading from Javanese citronella oil on MRSA. The intended antibiofilm is a barrier to biofilm formation and eradication. Bacteriostatic and antibiofilm effects were tested using microtiter plates assay, bactericidal effect test with subculture into the media and anticolony spreading effect test with spot inoculation in Tryptic Soy Broth media supplemented with 0.24% agar. The bacteriostatic effect test data were analyzed using paired t-test, bactericidal effect using the Friedman test, antibiofilm effect test using Kruskall-Wallis and the results of all the tests correlated using Pearson and Spearman correlation. The statistical significance used was p<0.05. The results showed that Javanese citronella oil had a bacteriostatic concentration of 0.02% (v/v) and bactericidal concentration of 0.78% (v/v). The Pearson correlation test showed that there was a negative correlation between bacteriostatic and bactericidal effects on biofilm formation with r=-0.956 (p=0.000), but the correlation was positive for biofilm eradication with r=0.918 (p=0.000) and anticolony spreading with r=1.000 (p=0.000).
The Effect of Hypercholesterolemia on Cortical Bone Thickness of Wistar Rats (Rattus norvegicus)
Authors: Sofka Auliya Dzaqiyatus , Lestari Pudji , Sari Gadis Meinar
Published: February 2020

There have been several studies reporting the effect of hypercholesterolemia on cortical thickness, but it remains a controversy. Some studies suggest that hypercholesterolemia can decrease osteoblast activity and increase osteoclast activity. Meanwhile other studies suggest that hypercholesterolemia is a protective factor of osteoporosis. Therefore, it is necessary to conduct a study to determine the effect of hypercholesterolemia on cortical bone thickness. Method: This study used 8 rats (Rattus norvegicus) that were divided into 2 groups, including control group (K0) that was given standard diet and experimental group (K1) that was given high fat diet for 28 days and all were executed to obtain the femur bone. The cortical bone thickness was measured by using Optilab Viewer and Optilab Image Raster software. Result: The data analysis was conducted using independent t-test. A p value of <0.05 is considered significant. The results showed that hypercholesterolemia had significant effect on cortical bone thickness. The average cortical bone thickness in the control group was 146.92 µm whereas in the experimental group was 124.53 µm, the mean difference between the two groups was 22.39 µm. There was a 6% decrease of cortical bone thickness. In conclusion, hypercholesterolemia can decrease cortical bone thickness of wistar rats (Rattus norvegicus).
The Genotype of Human Papilloma Virus of Male Patient with Anogenital Warts
Authors: Murtiastutik Dwi , Mastutik Gondo , Rahniayu Alphania , Arista Afria , Setyaningrum Trisniartami
Published: February 2020

Human papillomavirus (HPV) infection is one of the most common sexually transmitted infections. HPV high risk (HPVHR) were HPV16,18 related with invasive penile carcinomas, and HPV low risk (HPVLR) were HPV6,11 related to anogenital warts. Male infection is usually asymptomatic that it would be explain increasing the incidence of HPV associated cancers. Identification HPV genotype is very important for predicting the development of the diseases, to be benign or malignant cancer. The objective of this study was to identify the genotype of HPV that infect men with anogential warts. This research used 12 biopsy specimens from men patient with anogenital warts at Outpatient clinic of Department Dermatology and Venereology, Dr. Soetomo General Hospital period 2016-2017. The specimens were diagnozed by pathologist and HPV gentoyping was done to detect 40 HPV genotype including HPVHR and HPVLR. The result showed that 58% (7/12) were positive for HPVLR and 42% (5/12) were positive for HPV LR/HR. The genotype HPV that infected men patient with anogenital warts is HPVLR (HPV6,11) and HPVHR (HPV18,51,52,82) with single infection of HPVLR or mutiple infection HPVLR/LR or HPVLR/HR. The infection of HPVHR would be develops to be malignant transformation. It suggested that HPV genotype needs to be checked the for the anogenital warts cases for predicting the development of the diseases.
Correlation of Mothers with History of Diabetes Mellitus and Infants with Anti-GAD65
Authors: Salamy Nanda Fadhilah Witris , Sari Gadis Meinar , Purwanto Bambang , Sulistiawati Sulistiawati
Published: February 2020

This study aimed to determine the relationship between mothers with history of diabetes mellitus with Infants with Anti-GAD65. This study was an observational analytic study with a cohort study design. The case studied was the relationship between maternal history of diabetes mellitus and infants with Anti-GAD65. This study was conducted at Jemursari Hospital in Surabaya. Sample examination was performed with a GAD65 autoimmune rapid test. Then, a statistical test was performed to determine its relationship with other variables. There was no relationship between mothers with history of diabetes mellitus and infants with Anti-GAD65, but there was a significant relationship between Anti-GDA65 Mothers with Infants with Anti-GAD65. Thus, there was a possibility of transplacental antibody transfer and viral infections during pregnancy that cause damage to pancreatic beta cells. History of diabetes mellitus was not related to infants with Anti-GAD65, but there was a relationship between Anti-GAD65 Mothers with Anti-GAD65 BAyi so that there is a transfer of transplacenta antibodies and viral infections during pregnancy that can cause damage to beta pancreatic cells in infants.
THE ROLE OF PLATELET-RICH FIBRIN FACILITATES THE HEALING OF GASTROCNEMIUS MUSCLE DEFECT: A PRELIMINARY STUDY ON ANIMAL MODEL
Authors: Utomo Dwikora Novembri , Hernugrahanto Kukuh Dwiputra
Published: February 2020

Background: Scar formation has been a classical issue in the healing of skeletal muscle defect which will results in decrease of strength and function. Platelet-rich plasma (PRP) has been studied to enhance the healing of muscle defect. The disadvantages of it include the risk of immunologic reaction, time-consuming preparation, and lack of scaffold element. Platelet-rich fibrin (PRF) is a new generation of platelet concentrate that provides good source of growth factors and scaffold element necessary for the healing. This study is to provide a fondation of PRF preparation and implantation for the healing of experimental defect of gastrocnemius muscle in an animal model.Purpose: The goal is to provide a fondation of PRF preparation and implantation for the healing of experimental defect of gastrocnemius muscle in an animal model.Methods: Animal Care and Use Committee, Airlangga University approved the study. Twenty New Zealand white rabbits were divided into two groups. In control group, a defect was created in the right gastrocnemius and no implantation was done. In study group, similar defect was created with implantation of PRF. Two and four weeks after surgery, the defect was examined immunohistochemically for the expression of Pax7 protein.Results: The implanted group showed higher IRS score in two and four weeks compared to the control group.Conclusion: The use of PRF facilitates the healing of muscle injury. This study will be used as a fondation for further study on PRF.
THE EFFECT OF DECELLULARIZATION TECHNIQUE ON COLLAGEN TYPE II AND MATRICES POROSITY OF CARTILAGE BOVINE SCAFFOLD
Authors: Utomo Dwikora Novembri , Sari Ika Benny Kartika
Published: February 2020

Background: Cartilage has limited capacity for self-repair in case of defect. Stem cell based tissue engineering has become an alternative therapy for the treatment of cartilage defect. The use of decellularized cartilage bovine can serve as scaffold to support proliferation dan differentiation of the stem cell.Purpose: This research is to compare the effect of decellularization technique on collagen type II and matrices porosity of cartilage bovine scaffold.Methods: This experimental laboratory research used cartilage bovine scaffold which was decellularized using physical, chemical and enzymatic techniques with different time and concentration variations, then collagen type II measurement was performed by immunohistochemical method and evaluation of porosity size with electron microscopy.Results: In the preparations with the highest concentration and the longest immersion time of 5% SDS for 72 hours, the decellularization process occurs perfectly. The collagen type II content was found to have significantly higher concentrations than the controls and other preparations, and have interconnected porosities in large size.Conclusion: High concentrations of collagen type II  and large diameter of pores after decellularization process in cartilage bovine scaffold confirm that this scaffold still has optimum biochemical cues that can be a good mechanical framework and microenvironment stem cell in effort to repair the cartilage defects. This result was expected to become a standard for the decellularization making of cartilage bovine scaffold.
RELATIONSHIP OF ACL INJURY WITH POSTERIOR TIBIAL SLOPE, INTERCONDYLAR NOTCH WIDTH RATIO, AGE, AND SEX
Authors: Priono Boby Harul , Utoyo Ghuna Arioharjo , Ismiarto Yoyos Dias
Published: February 2020

Posterior tibial slope (PTS) has been proposed as a potential risk factor for ACL injury; however, studies that examined this relationship have provided inconclusive and sometimes contradictory results. Further characterization of this relationship may enable the medical community to identify individuals at greater risk for ACL injury and possibly characterize an anatomic target during surgical reconstruction. Medical records of 20 patients who underwent MRI of the knee and athroscopy procedure between August 2016 and March 2017 were reviewed. Patients were separated into 2 groups: a study group of those subjects who had undergone surgery for ACL injury (n =10) and a control group of patients diagnosed without ACL Injury (n = 10). Demographic data were collected, and MRI images from both groups were analyzed using protactor android imaging software to obtain PTS measurements. Data then analyzed using SPSS v20. Data analysis demonstrated that the ACL injury group had significantly greater values for PTS (14.4 SD ± 3.5) compared with controls (10.1 SD ± 2.6). After stepwise elimination of non significant variables, the final t test independent determined that PTS (p value 0.08; p < 0.1) had statistically significant relationships with ACL injury. INW ratio, age, and sex were not demonstrated to be significant predictors of ACL injury in this final model. This study showed a relationship between increased PTS and ACL injury, which corroborates the findings of previously published studies. INW ratio, sex, and age showed no significant relationship with ACL injury.
TRAUMATIC CERVICAL SPINAL CORD INJURY. IS URGENT INTERVENTION SUPERIOR TO DELAYED INTERVENTION? A META-ANALYSIS EVALUATION
Authors: Martiana I Ketut , Permana Donny , Widhiyanto Lukas
Published: February 2020

Introduction: Cervical spine is the most mobile part of the human spine, thus making it the most vulnerable compared to all the other vertebral structures. Surgical procedures are usually performed within the first 24 hours, or 4-6 weeks after trauma in order to prevent any secondary trauma. The research was conducted to evaluate the amount of time of the surgical procedure towards the effectivity and improvement of the neurological status in the cervical injury or acute spinal cord injury (ASCI).Methods: A meta-analysis research which evaluate the effectivity of surgical procedure on cervical trauma/ASCI, with the database procured from PubMed, Embase, and Cochrane. The main parameter is the decompression procedure and the clinical outcome which were categorized. The time of surgery or decompression are categorized into “<24 hours” and “>24 hours”, the neurological outcome is categorized into “improvement” and “no improvement”. The data was presented in odd ratio (OR) and confidence interval (CI) and were further analyzed by forest plot.Results: From PubMed, there were 353 articles, Embase 2 articles, and Cochrane 594 articles, but only 3 articles which fulfilled the inclusion criteria. The comparison between the surgical procedure in the cervical <24 hours with the surgical procedure >24 hours was identified for this research. Statistically, there was a significant difference on the neurological status (OR=1,85; 95%CI=1,21-2,84; p<0,01).Conclusion: With meta-analysis background, early decompressive procedure <24 hours for cervical trauma patients produced a significantly better result in improving the neurological status compared to the late decompressive procedure >24 hours.
MODIFIED LAUTENBACH TECHNIQUE AND EXTERNAL FIXATION AS A TREATMENT FOR INFECTED NON-UNION OF THE SUBTROCHANTER FEMUR: A CASE REPORT
Authors: Edward Mouli , Wardhana Teddy Heri , Nasrullah Erfan
Published: February 2020

Infected non-union is still a challenging orthopedic case to treat. Up until present day, there is no any established protocol to treat infected non-union of femur. Infection at subtrochanter femur specifically poses extra challenge as aforementioned location is affected by great tension and compression force. In this report, we present a 20-yo woman with infected subtrochanter femur following open reduction internal fixation (ORIF)with angle blade plate (ABP) 95o. One stage surgery was performed involving implant removal, debridement, definite external fixation, and antibiotic delivery using modified Lautenbach technique. Four years after trauma, the patient was capable of independent mobilization without aid. No infection recurrence was found, and Haris Hip Score was 91 (excellent). Based on the result of this case, definite external fixation and modified Lautenbach technique in one stage surgery can be a viable option in treating infected non-union of subtrochanter femur.
Comparison of 3-weekly cisplatin versus 3-weekly carboplatin in patients with locally advanced nasopharyngeal carcinoma receiving concurrent chemoradiotherapy: A multicentre analysis
Authors: Dechaphunkul Arunee , Danchaivijitr Pongwut , Jiratrachu Rungarun , Dechaphunkul Tanadech , Sookthon Chatsuda , Jiarpinitnun Chuleeporn , Paoin Chudchanok , Setakornnukul Jiraporn , Suktitipat Bhoom , Pattaranutaporn Poompis , Geater Sarayuth Lucien , Ngaimphaiboon Nuttapong
Published: February 2020

Background Although concurrent chemoradiotherapy (CCRT) with high-dose cisplatin remains a standard of care for patients with locally advanced nasopharyngeal carcinoma (LA-NPC), carboplatin has alternatively been used, particularly for cisplatin-ineligible patients. However, the comparable efficacy of these two regimens remains unclear. The present study aimed to evaluate the efficacy and tolerability of 3-weekly carboplatin and 3-weekly cisplatin therapies. Methods From May 2005 to November 2014, we identified patients with LA-NPC treated with CCRT from a Thai multicentre head and neck cancer database. The patients were administered a chemotherapy (CT) regimen of either cisplatin (75–100 mg/m 2 ) or carboplatin (AUC-5 or 6). Patient tolerability and survival were analysed and compared between regimens. Results Overall, 780 patients with LA-NPC were identified. Of these, the 76 patients (9.7%) treated with carboplatin showed significantly more comorbidity and lower baseline creatinine clearance than those treated with cisplatin. Compared with the cisplatin group, a higher number of patients in the carboplatin group completed three planned cycles of CT during CCRT (88.2% vs 52.0%; p <0.001) and three planned cycles of adjuvant CT (92.1% vs 78.7%; p =0.004). Furthermore, 28% of patients in the cisplatin group, as opposed to only 2.8% in the carboplatin group, required a dose reduction of CT in the subsequent cycle due to toxicities (p <0.001). At the time of analysis, the 5-year disease-free survival rate was 59.0% and 59.0% (p =0.935) and the 5-year overall survival rate was 64.0% and 66.0% (p =0.530), in the cisplatin and carboplatin groups, respectively. Conclusions Carboplatin provided comparable efficacy to that of cisplatin but with better tolerability and could be considered as an alternative regimen, particularly in cisplatin-ineligible patients with LA-NPC. These findings still warrant a randomised phase III study to compare these two CT regimens.
Motorcycle-related trauma: effects of age and site of injuries on mortality. A single-center, retrospective study.
Authors: Granieri Stefano , Reitano Elisa , Bindi Francesca , Renzi Federica , Sammartano Fabrizio , Cimbanassi Stefania , Gupta Shailvi , Chiara Osvaldo
Published: February 2020

Background

Motorcyclists are often victims of road traffic incidents. Though elderly patients seem to have worse survival outcomes and sustain more severe injuries than younger patients, concordance in the literature for this does not exist. The aim of the study is to evaluate the impact of age and injury severity on the mortality of patients undergoing motorcycle trauma.

Methods

Data of 1725 patients consecutively admitted to our Trauma Center were selected from 2002 to 2016 and retrospectively analyzed. The sample was divided into three age groups: ≤ 17 years, 18-54 years and ≥ 55 years. Mortality rates were analyzed for the overall population and patients with Injury Severity Score (ISS) ≥ 25. Differences in survival among age groups were evaluated with Log-Rank test and multivariate logistic regression models were created to identify independent predictors of mortality.

Results

A lower survival rate was detected in patients older than 55 years (83,6% vs 94,7%; p = 0.049) and in those sustaining critical injuries (ISS ≥ 25, 61% vs 83% p = 0.021). Age ( p =0,027; OR: 1,03), ISS ( p <0,001; OR: 1,09), Revised Trauma Score (RTS) ( p <0,001; OR: 0,47) resulted independent predictors of death. Multivariate analysis identified head ( p <0,001; OR: 2,04), chest ( p <0,001; OR: 1,54), abdominal ( p <0,001; OR: 1,37) and pelvic ( p =0,014; OR: 1,26) injuries as independent risk factors related to mortality as well. Compared to the theoretical probability of survival, patients of all age groups showed a survival advantage when managed at a level I Trauma Center.

Conclusions

We detected anatomical injury distributions and mortality rates among three age groups. Patients aging more than 55 years had an increased risk of death, with a prevalence of severe chest injuries, while younger patients sustained more severe head trauma. Age represented an independent predictor of death. Management of these patients at a Level I Trauma Center may lead to improved outcomes.
Effects of balance training on functional outcomes after total knee arthroplasty: a systematic review and meta-analysis of randomized controlled studies
Authors: Chen Wang , Hu Zheng-Hao , Sun Jian-Ning , Zhang Ye , Zhang Yu , Chen Xiang-yang , Feng Shuo
Published: February 2020

Background: Many previous studies have compared the effects of preoperative balance training and non-balance training on daily performance and knee functional outcomes after surgery, however, whether preoperative balance training is more beneficial to patients is still a big debate. Comparing the postoperative joint and daily function of balance training and non-balance training is the main purpose of our study.Methods: Cochrane library, Pubmed and Web of Science databases searched by us, and searched again before submitting our submission. This mete-analysis included 22 studies that directly compared postoperative performance and functional outcomes after training group (TG) and control group (CG). We used the software endnote X9 for data selection, and the software Review Manager 5.3 for data analysis to make funnel plots and forest plots.Results: The pooled data indicated that balance training significantly improved 2/6 MWT (2/6-min walk test) (weighted mean difference (WMD) = 25.17, 95% confidence interval (CI) 12.88 to 37.46, P < 0.0001), gait speed (WMD = 0.15, 95% CI 0.10 to 0.19, P < 0.00001), TUG (timed up and go) (WMD = 1.02, 95% CI 0.75 to 1.29, P < 0.00001), BBS (berg balance scale) (WMD = 1.79, 95% CI 0.50 to 3.08, P = 0.006), FHR (ratio of functional reach distance to body height) (WMD = 9.34, 95% CI 6.69 to 11.98, P < 0.00001), TCS (WMD = 1.20, 95% CI 0.86 to 1.53, P < 0.00001), early stage vitality (WMD = 14.41, 95% CI 13.53 to 15.30, P < 0.00001) and KOOS (Knee Injury and Osteoarthritis Score) sysptoms (WMD = 6.34, 95% CI 2.07 to 10.60, P = 0.004), middle stage function (WMD = 5.85, 95% CI 0.13 to 11.56, P = 0.04).Conclusion: Preoperative balance training improved partial postoperative daily performance and knee functional outcomes in patients with total knee arthroplasty.
Sediment microbial communities contribute to shrimp intestine microbiota in cultural pond ecosystems
Authors: Huang Zhijian , Hou Dongwei , Zhou Renjun , Zeng Shenzheng , Xing Chengguang , Wei Dongdong , Deng Xisha , Yu Lingfei , Wang Hao , Deng Zhixuan , Weng Shaoping , Ning Daliang , Xiao Chuanle , Yan Qingyun , Zhou Jizhong , He Zhili , He Jianguo
Published: February 2020

Background

Microorganisms in a shrimp cultural pond ecosystem (SCPE) play important roles for animal and environmental health. While the microbial diversity and assembly mechanism, and how communities of multiple habitats contribute to the SCPE metacommunity are poorly understood. Here, we analyzed phylogenetic structure of 792 samples of three habitats (water, shrimp intestine and sediment) from 88 shrimp cultural ponds across six regional sites in China.

Results

We show that the microbial communities of three habitats in the SCPE contained 15,197 operational taxonomic units (OTUs), and the microbial community of sediment had the highest OTUs (14,857). Despite the high microbial diversity, the SCPE has small core taxa (13 OTUs) among three habitats, and 30, 15, 53 core OTUs in water, shrimp intestine, and sediment, respectively. Especially, some opportunistic pathogens or functional microbes were core taxa in shrimp intestine or water and sediment. Of the SCPE metacommunity, the microbial diversity of the SCPE was mainly contributed by‾αLocalCommunities (66.0%), and followed by βInterHabitats (29.0%). The sediment microbial communities had a larger contribution (56.8%) to shrimp intestine microbiota compared to water communities (18.1%), which was also corroborated by the Sloan neutral community model analysis. Further, microbial assembly of three habitats appeared to be largely driven by stochastic processes (over 78%) in the SCPE.

Conclusions

Our results demonstrate that those core microbial taxa of three habitats were distinct with each other, that sediment communities mainly contributed to shrimp intestine communities, and that the microbial assembly was largely driven by stochastic processes. Our study enhances the mechanistic understanding of the microbial diversity and assembly in the SCPE for further analyzing metacommunities, and has important implications for microbial ecology and shrimp culture strategies.
An innovate strategy to treat large metaphyseal segmental femoral bone defect using customized design and 3D printed micro-porous prosthesis: a prospective clinical study
Authors: Hou Guojin , Liu Bingchuan , Tian Yun , Liu Zhongjun , Zhou Fang , Ji Hongquan , Zhang Zhishan , Guo Yan , Lv Yang , Yang Zhongwei
Published: February 2020

Background Large segmental bone defect at the metaphyseal area is still difficult to treat, nowadays, there is a tremendous level of interest in uses of 3D printing technology in orthopaedic surgery. This study was introduced to prospectively confirm the safety and effectiveness of 3D printed micro-porous prosthesis in clinical bone defect reconstruction application. Methods Patients with segmental irregular-shaped bone defect of the femur were recruited from 2017.12 to 2018.11. The first stage of the treatment involves radical debridement of all infected or non-viable bone and interposed fibrous tissue, and temporary fixation. Once the culture and biopsy results were negative, the PMMA spacer should remain in the defect approximately 6-8 weeks. This period is for the membrane formation, virtual surgery (computed tomography (CT) scan of the lesion area and the contra-lateral parts of the femur, and then design of the implant). The second stage involves reconstruction the defects with the 3D printed micro-porous prosthesis combined with intra-medullar nailwithout bone graft.Routine clinical follow-up and radiographic evaluation were done to assess bone incorporation and complications of internal fixation. The weight-bearing time and the joint function were recorded. Result 5 consecutive patients were included in the study. They were followed up for an average of 16.4 months. The average length of bone defect and the distal residual bone was 12 cm and 6.5 cm. The average time of partial weight-bearing and full weight-bearing was 12.7 days and 2.6 months. X-ray demonstrated good osseous integration of the implant/bone interface. No complications occurred such as implant loosening, subsidence, loss of correctionand infection. At the last follow-up, Harris score of hip joint was excellent in 2 cases, good in 2 cases, fair in 1 case; HSS score of knee joint was good in 4 cases, middle in 1 case. Conclusion Meticulous customized design 3D printed micro-porous prosthesis combined with intramedullary fixation may be a cost-effective and an alternative strategy to treat metaphyseal segmental irregular-shaped femoral bone defect without bone graft, especially for cases with massive juxta-articular bone loss.
Hepatic arterial infusion chemotherapy versus Sorafenib for advanced hepatocellular carcinoma with portal vein tumor thrombus: a systematic review and meta-analysis
Authors: ouyang guoqing , Pan Guangdong , Liu Qiang , Wu Yongrong , Tan shengqiang , Lu Wuhang , Li Shuai , xu honglai
Published: February 2020

Background : Hepatocellular carcinoma (HCC) is ranked as the sixth most common solid cancer and the third leading cause of cancer-related death in the world. Sorafenib is the first line systematic treatment for patients with advanced HCC. Hepatic arterial infusion chemotherapy（HAIC）has been proved to be an effective treatment for advanced HCC. Here, we conducted a meta-analysis to compared the efficacy of HAIC versus sorafenib of advanced HCC patients with PVTT. Methods : The databases of MEDLINE (PubMed), Cochrane Library, EMBASE, and Web of Science were systematically searched for retrieving the relevant publications before 31 July 2019. The endpoint included overall survival (OS), time to progression (TTP), partial response rate (PRR), complete response rate (CRR), objective response rate (ORR), stable disease rate (SDR). Results : A total of three studies involving 214 advanced HCC patients with PVTT enrolled in this meta-analysis. HAIC significantly improved TTP (hazard ratio (HR) = 0.56, 95% CI: 0.39-0.82; P = 0.003), PRR (odds ratio (OR) = 3.31, 95% CI: 1.46-7.50; P = 0.004), ORR (OR = 3.78, 95% CI: 1.68-8.50; P = 0.001) compared to sorafenib. However, no significant difference was found in OS (HR=0.77, 95%CI: 0.56-1.06, p=0.11), CRR (OR = 2.54, 95% CI: 0.39-16.47; P = 0.33), SDR (OR = 1.48, 95% CI: 0.43-5.08; P = 0.001). Conclusions : This meta-analysis suggested that HAIC provides better TTP, PRR, ORR than sorafenib for patients of advanced HCC with PVTT. Therefore, we recommend HAIC as a potential therapy for advanced HCC patients with PVTT. However, owing to the above limitations, more high-quality studies are warranted to evaluate this finding.
Quercetin promotes long noncoding RNA lncSHGL expression via estrogen receptor α to suppress type 2 diabetes mellitus in mice
Authors: Fan Hanlu , Li Haiwen , Liu Huijiao , Li Peng , Jia Xiaomeng , Guo Peilan , Yu Qingquan , Li Xiru , Guo Yangdong , Li Xiangdong
Published: February 2020

Background

Type 2 diabetes mellitus (T2DM) is the most common type of metabolic disorder involving multiple organ systems. Grape has been reported to improve the symptoms of T2DM, the precise mechanism of its action is unclear. Our study was aimed to determine the effect and mechanism of grape pomace extract in T2DM mice induced by high fat diet (HFD).

Materials and methods

Ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry were used to identify the main active compounds in grape pomace extract to improve T2DM. C57BLK/6J mice induced by HFD supplemented with or without quercetin were used to show the effects of quercetin improving T2DM. By online database research, bioinformatics analysis and molecular biology experiments, Estrogen receptor alpha (ERα)-lncSHGL (lncRNA suppressor of hepatic gluconeogenesis and lipogenesis) pathway was identify as the target for quercetin.

Results

Quercetin was identified as one of the most active compounds in grape pomace extract to improve T2DM. Quercetin could inhibit HFD-induced T2DM in mice by activing ERα. LncSHGL was identified as the downstream of ERα and inhibited HFD-induced T2DM.

Conclusions

Quercetin could be beneficial for T2DM by promoting lncSHGL transcription and activating the lncSHGL pathway, and may be used as a drug component to treat T2DM.
Epidemic analysis of COVID-19 in China after Wuhan was restricted
Authors: yu anfeng , wang zhiwei , ren wei , wu zhiyong , hu zhipeng , li luocheng , ruan yongle , hu rui , shi feng
Published: February 2020

BackgroundIn December 2019, Coronavirus Disease 2019(COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection appeared in Wuhan, Hubei Province, China. The disease is highly infectious. Wuhan, Hubei Province decided restrict personnel movement on January 23.We analyze relevant data to show the situation of the COVID-19 epidemic in China.MethodsThe data was classified according to Hubei group, non-Hubei group, Hong Kong, Macao and Taiwan group, and Chinese Mainland group, and analyze the current situation and trend of the epidemic.ResultsThere was an explosive growth in the early stage of the epidemic. The epidemic situation began to improve in about two weeks after Wuhan was restricted,and the situation in non-Hubei was significantly better than that in Hubei.ConclusionThe blockade of Wuhan was a correct decision, cut off the outflow of tinfection sources, and the epidemic situation in all places turned around after the incubation period.
Efficacy and safety of different mechanical ventilation strategies for patients with acute respiratory distress syndrome: Systematic review and network meta-analysis
Authors: Huang Wenqi , Wang Pengfei , Jia Fang , Huang Bin , Chen Xiaodong , Du Hu , Sun Hang , Liu Qi , Zhang An
Published: February 2020

Background: Acute respiratory distress syndrome (ARDS) leads to life-threatening acute hypoxemic respiratory failure in clinic and usually requires mechanical ventilation. Low tidal volume ventilation is now universally accepted as management treatment for this condition. However, recruitment maneuvers (RM) and positive end-expiratory pressure (PEEP) remain controversial. Therefore, we performed a network meta-analysis to identify the RM and PEEP levels of patients with ARDS.Methods: We searched PubMed, OVID, Embase, and the Cochrane Central Register of Controlled Trials (Central) databases. The primary outcome was death in the 28th day, and the secondary outcomes included death in hospital, ventilator-free days, and barotrauma. Data for study characteristics, methods, and outcomes were extracted. The relative effect sizes were estimated by risk ratios (RRs) for binary variables and standard mean difference (SMD) for continuous variables. Relative ranking of the interventions was conducted using surface under the cumulative ranking. Multiple intervention comparisons based on the Bayesian and frequentist frameworks were performed to integrate the efficacy of all included strategies.Results: Thirty randomized controlled trials comprising 4410 patients were included in the network meta-analysis. None of the ventilation strategies was significantly superior over others for all outcomes. According to the relative rank probabilities, low PEEP showed the lowest probability of harming death in the 28th day, whereas RM+low PEEP showed the highest probability of benefitting death in hospital and ventilator-free days. Low PEEP showed the highest probability of benefitting barotrauma. The overall quality of the evidence per grade was moderate to low.Conclusions: No ventilation strategy is significantly superior over others. RM+low PEEP has the highest probability of benefitting survival. The evidence has low overall quality and should be further studied.