bluuebunny/crossref_metadata_2025 huggingface dataset:
Stereoelectronic Features of a Complex Ketene Dimerization Reaction
Authors: Barrows Robert D. , Dresel Mark J. , Emge Thomas J. , Rablen Paul R. , Knapp Spencer
Published: December 2021

The amidation reaction of a tetrahydroisoquinolin-1-one-4-carboxylic acid is a key step in the multi-kilogram-scale preparation of the antimalarial drug SJ733, now in phase 2 clinical trials. In the course of investigating THIQ carboxamidations, we found that propanephosphonic acid anhydride (T3P) is an effective reagent, although the yield and byproducts vary with the nature and quantity of the base. As a control, the T3P reaction of a 3-(2-thienyl) THIQ was performed in the absence of the amine, and the products were characterized: among them are three dimeric allenes and two dimeric lactones. A nucleophile-promoted ketene dimerization process subject to subtle steric and stereoelectronic effects accounts for their formation. Two novel monomeric products, a decarboxylated isoquinolone and a purple, fused aryl ketone, were also isolated, and mechanisms for their formation from the ketene intermediate are proposed.
The Impact of Retinal Configuration on the Protein–Chromophore Interactions in Bistable Jumping Spider Rhodopsin-1
Authors: Church Jonathan R. , Olsen Jógvan Magnus Haugaard , Schapiro Igor
Published: December 2021

Bistable rhodopsins have two stable forms that can be interconverted by light. Due to their ability to act as photoswitches, these proteins are considered as ideal candidates for applications such as optogenetics. In this work, we analyze a recently crystalized bistable rhodopsin, namely the jumping spider rhodopsin-1 (JSR1). This rhodopsin exhibits identical absorption maxima for the parent and the photoproduct form, which impedes its broad application. We performed hybrid QM/MM simulations to study three isomers of the retinal chromophore: the 9-cis, 11-cis and all-trans configurations. The main aim was to gain insight into the specific interactions of each isomer and their impact on the absorption maximum in JSR1. The absorption spectra were computed using sampled snapshots from QM/MM molecular dynamics trajectories and compared to their experimental counterparts. The chromophore–protein interactions were analyzed by visualizing the electrostatic potential of the protein and projecting it onto the chromophore. It was found that the distance between a nearby tyrosine (Y126) residue plays a larger role in the predicted absorption maximum than the primary counterion (E194). Geometric differences between the isomers were also noted, including a structural change in the polyene chain of the chromophore, as well as changes in the nearby hydrogen bonding network.
Total Release of 21 Indicator Pharmaceuticals Listed by the Swedish Medical Products Agency from Wastewater Treatment Plants to Surface Water Bodies in the 1.3 Million Populated County Skåne (Scania), Sweden
Authors: Björklund Erland , Svahn Ola
Published: December 2021

In 2017, the Swedish Environmental Protection Agency published a report on advanced wastewater treatment for the removal of pharmaceutical residues and stated that advanced treatment should be implemented where it will make the largest difference from an environmental perspective. However, the report also concluded that this need cannot be specified with existing data, but consideration must be made of local conditions. Two considerations are (1) the discharged amount of pharmaceutical into receiving water bodies and (2) the turnover of water in the recipient, where the highest risks are related to recipients with a low water turnover and low dilution. The current project comprised eight different WWTPs distributed throughout the entire County Skåne (Scania) in Sweden, with a population of ca. 1,300,000 persons. In total, 21 of 22 pharmaceuticals were analyzed according to the list proposed by the Swedish Medical Products Agency 2015. The results show that large amounts of pharmaceuticals are released from the WWTPs yearly to Scanian recipients. The total discharge of pharmaceuticals from the eight treatment plants adds up to 71 kg of these 21 substances alone, mainly comprising metoprolol, which is a drug that lowers blood pressure, and the analgesic drug diclofenac. Additionally, carbamazepine, losartan, naproxen and oxazepam were present in significant concentrations. These represented three illnesses that are very common: high blood pressure, inflammation/pain and depression/anxiety. The concentrations were generally in line with previous national Swedish screenings. It was estimated that, when one million cubic meters (1,000,000 m3) of wastewater is discharged, almost 4 kg of the 21 pharmaceuticals is released. The total volume wastewater release by the >90 WWTPs in Scania was estimated to 152,887,000 m3, which corresponded to 590 kg/year. The investigated 21 drugs cover only a small part of many hundred pharmaceuticals that are in use in Sweden. Thus, most likely, one or several tons of pharmaceuticals leak out to the Scanian recipients annually. The analysis of river samples shows that the dilution of wastewater is a key parameter in reducing concentrations. However, some locations have remarkably high concentrations, which occur when the volume wastewater is large in relation to the flow in the river. These kinds of regional results are of importance when selecting where advanced treatment should be prioritized in a first instance, as requested by the Swedish EPA.
Rosmanol and Carnosol Synergistically Alleviate Rheumatoid Arthritis through Inhibiting TLR4/NF-κB/MAPK Pathway
Authors: Li Lianchun , Pan Zhenghong , Ning Desheng , Fu Yuxia
Published: December 2021

Callicarpalongissima has been used as a Yao folk medicine to treat arthritis for years in China, although its active anti-arthritic moieties have not been clarified so far. In this study, two natural phenolic diterpenoids with anti-rheumatoid arthritis (RA) effects, rosmanol and carnosol, isolated from the medicinal plant were reported on for the first time. In type II collagen-induced arthritis DBA/1 mice, both rosmanol (40 mg/kg/d) and carnosol (40 mg/kg/d) alone alleviated the RA symptoms, such as swelling, redness, and synovitis; decreased the arthritis index score; and downregulated the serum pro-inflammatory cytokine levels of interleukin 6 (IL-6), monocyte chemotactic protein 1 (MCP-1), and tumor necrosis factor α (TNF-α). Additionally, they blocked the activation of the Toll-like receptor 4 (TLR4)/nuclear factor κB (NF-κB)/c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) pathways. Of particular interest was that when they were used in combination (20 mg/kg/d each), the anti-RA effect and inhibitory activity on the TLR4/NF-κB/MAPK pathway were significantly enhanced. The results demonstrated that rosmanol and carnosol synergistically alleviated RA by inhibiting inflammation through regulating the TLR4/NF-κB/MAPK pathway, meaning they have the potential to be developed into novel, safe natural combinations for the treatment of RA.
Investigation of the Variability of Alkaloids in Buxus sempervirens L. Using Multivariate Data Analysis of LC/MS Profiles
Authors: Szabó Lara U. , Schmidt Thomas J.
Published: December 2021

Buxus sempervirens L. is a common ornamental plant in southern and central Europe, and has been used ethopharmacologically against a wide variety of diseases due to it containing nor-triterpene alkaloids of the nor-cycloartane type. Recently, we demonstrated the interesting antiprotozoal potential of some of these compounds. To characterize the temporal variability in the alkaloid profile of two different varieties and their leaves and twigs, 30 different extracts of B. sempervirens were evaluated by Ultra High Performance Liquid Chromatography/positive Mode-Electrospray Ionization Quadrupole Time-of-Flight-Tandem Mass Spectrometry (UHPLC/+ESI-QqTOF-MS/MS). The analytical profiles were thoroughly investigated by various methods of multivariate data analysis (MVDA). A principal component analysis (PCA) model elucidates the seasonal variation in the phytochemical composition of B. sempervirens var. arborescens and suffruticosa along with differences between the varieties. Analysis of a volcano plot illustrated the differences between the two organs, the leaf and twig. Eighteen compounds were highlighted by the models as constituents of the plant characteristic for a season, variety or organ. These compounds were dereplicated based on their chromatographic and +ESI-QqTOF-MS and –MS/MS data. In addition, mass spectral fragmentation pathways for already known alkaloids as well as new natural products could be postulated for the first time. In conclusion, the MVDA models give detailed information on the temporal variability in the alkaloid profile of two different varieties and their organs (leaf vs. twig) of B. sempervirens. Thus, the results of this study allow, e.g., the identification of characteristic compounds for the different varieties, plant organs, seasons, and the optimal harvesting time for the isolation of particular Buxus-alkaloids of interest for subsequent studies.
Evaluation of 2-Thioxoimadazolidin-4-one Derivatives as Potent Anti-Cancer Agents through Apoptosis Induction and Antioxidant Activation: In Vitro and In Vivo Approaches
Authors: Nafie Mohamed S. , Khodair Ahmed I. , Hassan Hebat Allah Y. , El-Fadeal Noha M. Abd , Bogari Hanin A. , Elhady Sameh S. , Ahmed Safwat A.
Published: December 2021

Background: Hepatocellular carcinoma (HCC) is one of the most widespread malignancies and is reported as the fourth most prevalent cause of cancer deaths worldwide. Therefore, we aimed to investigate the probable mechanistic cytotoxic effect of the promising 2-thioxoimidazolidin-4-one derivative on liver cancer cells using in vitro and in vivo approaches. The compounds were tested for the in vitro cytotoxic activity using MTT assay, and the promising compound was tested in colony forming unit assay, flow cytometric analysis, RT-PCR, Western blotting, in vivo using SEC-carcinoma and in silico to highlight the virtual mechanism of action. Both compounds 4 and 2 performed cytotoxic effects against HepG2 cells with IC50 values of 0.017 and 0.18 μM, respectively, compared to Staurosporine and 5-Fu as reference drugs with IC50 values of 5.07 and 5.18 µM, respectively. Compound 4 treatment revealed apoptosis induction by 19.35-fold (11.42% compared to 0.59% in control), arresting the cell cycle at G2/M phase. Moreover, studying gene expression that plays critical roles in cell cycle and apoptosis by RT-PCR demonstrated that compound 4 enhances the expression of the pro-apoptotic genes p53, PUMA, and Caspase 3, 8, and 9, and impedes the anti-apoptotic Bcl-2 gene in the HepG2 cells. It can also inhibit the PI3K/AKT pathway at both gene and protein levels, which was reinforced by the in silico predictions of the molecular docking simulations towards the PI3K/AKT proteins. Finally, in vivo study verified that compound 4 has a promising anti-cancer activity through activating antioxidant levels (CAT, SOD and GSH) and ameliorating hematological, biochemical, and histopathological findings.
Nitrogen Estimation for Wheat Using UAV-Based and Satellite Multispectral Imagery, Topographic Metrics, Leaf Area Index, Plant Height, Soil Moisture, and Machine Learning Methods
Authors: Yu Jody , Wang Jinfei , Leblon Brigitte , Song Yang
Published: December 2021

To improve productivity, reduce production costs, and minimize the environmental impacts of agriculture, the advancement of nitrogen (N) fertilizer management methods is needed. The objective of this study is to compare the use of Unmanned Aerial Vehicle (UAV) multispectral imagery and PlanetScope satellite imagery, together with plant height, leaf area index (LAI), soil moisture, and field topographic metrics to predict the canopy nitrogen weight (g/m2) of wheat fields in southwestern Ontario, Canada. Random Forests (RF) and support vector regression (SVR) models, applied to either UAV imagery or satellite imagery, were evaluated for canopy nitrogen weight prediction. The top-performing UAV imagery-based validation model used SVR with seven selected variables (plant height, LAI, four VIs, and the NIR band) with an R2 of 0.80 and an RMSE of 2.62 g/m2. The best satellite imagery-based validation model was RF, which used 17 variables including plant height, LAI, the four PlanetScope bands, and 11 VIs, resulting in an R2 of 0.92 and an RMSE of 1.75 g/m2. The model information can be used to improve field nitrogen predictions for the effective management of N fertilizer.
Curcumin May Prevent Basement Membrane Disassembly by Matrix Metalloproteinases and Progression of the Bladder Cancer
Authors: Wroński Paweł , Wroński Stanisław , Kurant Marcin , Malinowski Bartosz , Wiciński Michał
Published: December 2021

Authors present a review of crucial mechanisms contributing to the invasion of the basement membrane (BM) of the urothelium by cancer cells and to the progression of bladder cancer (BC). The breeching of the urothelial BM, facilitated by an aberrant activation of matrix metalloproteinases (MMP) is particularly perilous. Inhibition of activation of these proteinases constitutes a logic opportunity to restrain progression. Because of limited efficacy of current therapeutic methods, the search for the development of alternative approaches constitutes “the hot spot” of modern oncology. Recent studies revealed significant anticancer potential of natural phytochemicals. Especially, curcumin has emerged as a one of the most promising phytochemicals and showed its efficacy in several human malignancies. Therefore, this article addresses experimental and clinical data indicating multi-directional inhibitory effect of curcumin on the growth of bladder cancer. We particularly concentrate on the mechanisms, by which curcumin inhibits the MMP’s activities, thereby securing BM integrity and alleviating the eventual cancer invasion into the bladder muscles. Authors review the recently accumulating data, that curcumin constitutes a potent factor contributing to the more effective treatment of the bladder cancer.
Adherence to the Japanese Food Guide: The Association between Three Scoring Systems and Cardiometabolic Risks in Japanese Adolescents
Authors: Okuda Masayuki , Fujiwara Aya , Sasaki Satoshi
Published: December 2021

The Japanese Food Guide Spinning Top (JFGST) indicates optimal intake of five food groups (grain, fish and meat, vegetables, milk, and fruits) and sugar and confectionaries. We aimed to investigate whether adherence to the JFGST in 8th grade junior high school students (n = 3162) was associated with cardiometabolic risks and how different scorings of the JFGST influenced the associations. Metabolic risks were assessed from anthropometrics, blood pressure measurements, and blood glucose and lipid profile measurements. Three types of scoring adherent to the JFGST were analyzed (10 points were given for each item with optimal intake; range: 0–60): the original scoring (ORG scoring); first modified scoring, which had no upper limits for vegetables and fruits (MOD1 scoring); and MOD2 scoring without upper limits for five dishes (MOD2 scoring). The MOD2 scoring was positively associated with dietary fiber, potassium, calcium, and vitamins. All types of scorings were associated with low glucose levels (p ≤ 0.001); the MOD2 scoring was associated with low systolic blood pressure (p = 0.001) and low cardiometabolic risk (p = 0.003). Our findings suggest that Japanese adolescents adherent to the JFGST had low cardiometabolic risks and should not fall below lower limits for intake of the abovementioned five food groups.
Malnutrition, Sarcopenia, and Malnutrition-Sarcopenia Syndrome in Older Adults with COPD
Authors: Kaluźniak-Szymanowska Aleksandra , Krzymińska-Siemaszko Roma , Deskur-Śmielecka Ewa , Lewandowicz Marta , Kaczmarek Beata , Wieczorowska-Tobis Katarzyna
Published: December 2021

Purpose: Chronic obstructive pulmonary disease (COPD) is the fourth leading cause of death in the world population. In addition to airflow obstruction, COPD is associated with multiple systemic manifestations, including impaired nutritional status or malnutrition and changes in body composition (low muscle mass, LMM). Poor nutritional status and sarcopenia in subjects with COPD leads to a worse prognosis and increases health-related costs. Data from previous studies indicate that 30–60% of subjects with COPD are malnourished, 20–40% have low muscle mass, and 15–21.6% have sarcopenia. This study aimed to assess the prevalence of malnutrition, sarcopenia, and malnutrition-sarcopenia syndrome in elderly subjects with COPD and investigate the relationship between COPD severity and these conditions.Patients and methods: A cross-sectional study involving 124 patients with stable COPD, aged ≥60, participating in a stationary pulmonary rehabilitation program. Nutritional status was assessed following the Global Leadership Initiative on Malnutrition (GLIM) criteria and sarcopenia with the European Working Group on Sarcopenia in Older People 2 (EWGSOP2) criteria. The results of pulmonary function tests and exercise capacity were obtained from the hospital database. Results: 22.6% of participants had malnutrition according to the GLIM criteria. Subjects with malnutrition had lower gait speed (p = 0.0112) and worse results of the Six Minute Walk Test. Sixteen participants (12.9%) had sarcopenia; 12 subjects with sarcopenia had concomitant malnutrition. The prevalence of severe and very severe obstruction (GOLD3/GOLD4) was 91.7%. It was significantly higher in patients with malnutrition-sarcopenia syndrome. Conclusions: Malnutrition was found in nearly one out of four subjects with COPD, while sarcopenia was one out of seven patients. About 10% of our study sample had malnutrition-sarcopenia syndrome. The prevalence of severe and very severe obstruction was significantly higher in patients with malnutrition-sarcopenia syndrome.
Association between Non-Alcoholic Fatty Liver Disease and Mediterranean Lifestyle: A Systematic Review
Authors: Mascaró Catalina M , Bouzas Cristina , Tur Josep A
Published: December 2021

Background and Aims: Non-alcoholic fatty liver disease (NAFLD) is an excessive accumulation of fat in the liver without alcohol abuse. It is linked to metabolic syndrome (MetS) and no pharmacological treatment exists. This systematic review aims to assess evidence about the effect of Mediterranean lifestyle on the prevention and reversion of NAFLD. Methods: A systematic literature search was performed in MEDLINE via Pubmed. MeSH terms used were: non-alcoholic fatty liver disease [MeSH Major Topic] AND metabolic syndrome [MeSH Term] AND (Diet, Mediterranean [MeSH Term]) OR (Exercise [MeSH Term]). (PROSPERO ID: 2021 CRD42021289495). Results: Thirteen articles were selected and divided into two categories (four focused on Mediterranean diet and NAFLD and nine focused on Mediterranean diet, physical activity, and NAFLD). Information of clinical endpoints was based on NAFLD, as well as MetS, body mass index, fasting glycemia, obesity, cholesterol, triglycerides, transaminases, albuminuria, and hepatic steatosis, among others. All studies found beneficial associations between the clinical parameters of NAFLD/MetS and following a Mediterranean diet and regular physical activity. Conclusions: An effective treatment that prevents, and even reverses, NAFLD is to adapt lifestyle to the Mediterranean one, based on a Mediterranean diet and regular physical activity.
Association between Health-Related Physical Fitness and Risk of Dyslipidemia in University Staff: A Cross-Sectional Study and a ROC Curve Analysis
Authors: Zhou Yuan , Zhang Jing , Liu Rong-Hua , Xie Qian , Li Xiao-Long , Chen Jian-Gang , Pan Xin-Liang , Ye Bo , Liu Long-Long , Wang Wan-Wan , Yan Liang-Liang , Wei Wen-Xin , Jiang Xin-Cheng
Published: December 2021

Background: This study aimed to assess the relationship between dyslipidemia (DL) risk and health-related physical fitness (HPF) and evaluated the prognostic value of HPF for risk of DL. Methods: A total of 776 university staff members were recruited, of which 407 were females, and 369 males. Blood samples and HPF tests were collected from all participants after 12 h fasting. Results: The prevalence of DL was 41.77% and 51.49% in female and male university staff members, respectively, and there was no significant difference between genders (χ2 = 2.687, p = 0.101). According to the logistic regression analysis, age, male sex, GLU, hypertension, BMI, BF, WHtR, and LAP were significant risk factors for DL (p < 0.05), VCI and, SAR were significant protective factors for DL (p < 0.05), and SMI, GS, and VG were not significantly associated with the risk of DL. The area under the receiver-operating characteristic (ROC) curve (AUC) analysis indicated that, LAP (AUC: 0.730, 95CI%: 0.697–0.762), WHtR (AUC: 0.626, 95CI%: 0.590–0.660), and BMI (AUC: 0.599, 95CI%: 0.563–0.634) are valid predictors of DL, and LAP and WHtR perform better than BMI (Z = 8.074, p < 0.001) in predicting DL in male and female university staff members. Conclusion: The risk of DL is significantly related to body composition, cardiorespiratory fitness, and flexibility. LAP and WHtR perform better than BMI in predicting risk of DL in male and female university staff members.
Influence of Climatic Factors on Human Hantavirus Infections in Latin America and the Caribbean: A Systematic Review
Authors: Douglas Kirk Osmond , Payne Karl , Sabino-Santos Gilberto , Agard John
Published: December 2021

Background: With the current climate change crisis and its influence on infectious disease transmission there is an increased desire to understand its impact on infectious diseases globally. Hantaviruses are found worldwide, causing infectious diseases such as haemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS)/hantavirus pulmonary syndrome (HPS) in tropical regions such as Latin America and the Caribbean (LAC). These regions are inherently vulnerable to climate change impacts, infectious disease outbreaks and natural disasters. Hantaviruses are zoonotic viruses present in multiple rodent hosts resident in Neotropical ecosystems within LAC and are involved in hantavirus transmission. Methods: We conducted a systematic review to assess the association of climatic factors with human hantavirus infections in the LAC region. Literature searches were conducted on MEDLINE and Web of Science databases for published studies according to Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) criteria. The inclusion criteria included at least eight human hantavirus cases, at least one climatic factor and study from > 1 LAC geographical location. Results: In total, 383 papers were identified within the search criteria, but 13 studies met the inclusion criteria ranging from Brazil, Chile, Argentina, Bolivia and Panama in Latin America and a single study from Barbados in the Caribbean. Multiple mathematical models were utilized in the selected studies with varying power to generate robust risk and case estimates of human hantavirus infections linked to climatic factors. Strong evidence of hantavirus disease association with precipitation and habitat type factors were observed, but mixed evidence was observed for temperature and humidity. Conclusions: The interaction of climate and hantavirus diseases in LAC is likely complex due to the unknown identity of all vertebrate host reservoirs, circulation of multiple hantavirus strains, agricultural practices, climatic changes and challenged public health systems. There is an increasing need for more detailed systematic research on the influence of climate and other co-related social, abiotic, and biotic factors on infectious diseases in LAC to understand the complexity of vector-borne disease transmission in the Neotropics.
Acceptability of Mebendazole Chewable Tablet in Children Aged 2 to 4 Years in Peru
Authors: Perez Fernando , Vallet Thibault , Bravo Zarela , Callahan Kristin , Ruiz Fabrice
Published: December 2021

Soil-transmitted helminthiasis (STH) is among the most common of parasitic infections, affecting vulnerable populations in tropical/subtropical areas globally. In endemic countries, children, a high-risk population, require treatment and preventive interventions. Mebendazole, a WHO-recommended medicine, originally formulated as a tablet that was often crushed for administration to young children unable to swallow it, was reformulated as a chewable tablet. Acceptability is a key aspect for treatment effectiveness in pediatrics. Herein, we used a validated data-driven approach to investigate the acceptability of the 500-mg mebendazole chewable tablet in children aged 2 to 4 years in Peru. Observer-reported outcomes were collected for 182 medicine intakes. Acceptability was scored using the acceptability reference framework: a three-dimensional map juxtaposing “positively accepted” and “negatively accepted” profiles. Results found that the 500-mg mebendazole chewable tablet was classified as “positively accepted” in children aged 2 to 4 years. Acceptability increased with age and some acceptability issue remain for the younger children. Nevertheless, this formulation was considerably better accepted than the conventional tablets regardless of treatment in young children. This chewable formulation appears to be an appropriate alternative to the hard tablet of mebendazole for treatment of STH and preventive interventions in children aged 2 to 4 years.
Red Seaweed Pigments from a Biotechnological Perspective
Authors: Freitas Marta V. , Pacheco Diana , Cotas João , Mouga Teresa , Afonso Clélia , Pereira Leonel
Published: December 2021

Algae taxa are notably diverse regarding pigment diversity and composition, red seaweeds (Rhodophyta) being a valuable source of phycobiliproteins (phycoerythrins, phycocyanin, and allophycocyanin), carotenes (carotenoids and xanthophylls), and chlorophyll a. These pigments have a considerable biotechnological potential, which has been translated into several registered patents and commercial applications. However, challenges remain regarding the optimization and subsequent scale-up of extraction and purification methodologies, especially when considering the quality and quantity needs, from an industrial and commercial point of view. This review aims to provide the state-of-the-art information on each of the aforementioned groups of pigments that can be found within Rhodophyta. An outline of the chemical biodiversity within pigment groups, current extraction and purification methodologies and challenges, and an overview of commercially available products and registered patents, will be provided. Thus, the current biotechnological applications of red seaweeds pigments will be highlighted, from a sustainable and economical perspective, as well as their integration in the Blue Economy.
Relationship between the Characteristics of Bread Wheat Grains, Storage Time and Germination
Authors: Afonnikov Dmitry A. , Komyshev Evgenii G. , Efimov Vadim M. , Genaev Mikhail A. , Koval Vasily S. , Gierke Peter U. , Börner Andreas
Published: December 2021

Seed storage is important to farmers, breeders and for germplasm preservation. During storage, seeds accumulate damage at the structural and metabolic level, which disrupt their function and reduce resistance to adverse external conditions. In this regard, issues related to seed aging prove to be relevant for maintaining the viability of genetic collections. We analyzed morphological characteristics of grains and their coat color for 44 recombinant inbred lines (RILs) of bread wheat grown in four different seasons, 2003, 2004, 2009 and 2014. Our investigations were performed in 2020. For 19 RILs from the same seasons germination was evaluated. Our results demonstrate that genotype significantly affects the variability of all seed traits, and the year of harvesting affects about 80% of them (including all the traits of shape and size). To identify the trend between changes in grain characteristics and harvesting year, we estimated correlation coefficients between them. No significant trend was detected for the grain shape/size traits, while 90% of the color traits demonstrated such a trend. The most significant negative correlations were found between the harvesting year and the traits of grain redness: the greater the storage time, the more intensive is red color component for the grains. At the same time, it was shown that grains of longer storage time (earlier harvesting year) have lighter coat. Analysis of linear correlations between germination of wheat seeds of different genotypes and harvesting years and their seed traits revealed a negative linear relationship between the red component of coat color and germination: the redder the grains, the lower their germination rate. The results obtained demonstrate manifestations of metabolic changes in the coat of grains associated with storage time and their relationship with a decrease of seed viability.
Tuning High and Low Temperature Foaming Behavior of Linear and Long-Chain Branched Polypropylene via Partial and Complete Melting
Authors: Kweon Mu Sung , Embabi Mahmoud , Shivokhin Maksim E. , Gupta Anvit , Yan Xuejia , Pehlert George , Lee Patrick C.
Published: December 2021

While existing foam studies have identified processing parameters, such as high-pressure drop rate, and engineering measures, such as high melt strength, as key factors for improving foamability, there is a conspicuous absence of studies that directly relate foamability to material properties obtained from fundamental characterization. To bridge this gap, this work presents batch foaming studies on one linear and two long-chain branched polypropylene (PP) resins to investigate how foamability is affected by partial melting (Method 1) and complete melting followed by undercooling (Method 2). At temperatures above the melting point, similar expansion was obtained using both foaming procedures within each resin, while the PP with the highest strain hardening ratio (13) exhibited the highest expansion ratio (45 ± 3). At low temperatures, the foamability of all resins was dramatically improved using Method 2 compared to Method 1, due to access to lower foaming temperatures (<150 °C) near the crystallization onset. Furthermore, Method 2 resulted in a more uniform cellular structure over a wider temperature range (120–170 °C compared to 155–175 °C). Overall, strong extensional hardening and low onset of crystallization were shown to give rise to foamability at high and low temperatures, respectively, suggesting that both characteristics can be appropriately used to tune the foamability of PP in industrial foaming applications.
Wood Dust Flammability Analysis by Microscale Combustion Calorimetry
Authors: Xu Qiang , Jiang Lin , Majlingova Andrea , Ulbrikova Nikoleta , Mensah Rhoda Afriyie , Das Oisik , Berto Filippo
Published: December 2021

To study the practicability of a micro combustion calorimeter to analyze the calorimetry kinetics of wood, a micro combustion calorimeter with 13 heating rates from 0.1 to 5.5 K/s was used to perform the analysis of 10 kinds of common hardwood and softwood samples. As a microscale combustion measurement method, MCC (microscale combustion calorimetry) can be used to judge the flammability of materials. However, there are two methods for measuring MCC: Method A and Method B. However, there is no uniform standard for the application of combustible MCC methods. In this study, the two MCC standard measurement Methods A and B were employed to check their practicability. With Method A, the maximum specific heat release rate, heat release temperature, and specific heat release of the samples were obtained at different heating rates, while for Method B, the maximum specific combustion rate, combustion temperature and net calorific values of the samples were obtained at different heating rates. The ignition capacity and heat release capacity were then derived and evaluated for all the common hardwood and softwood samples. The results obtained by the two methods have significant differences in the shape of the specific heat release rate curves and the amplitude of the characteristic parameters, which lead to the differences of the derived parameters. A comparison of the specific heat release and the net calorific heat of combustion with the gross caloric values and heating values obtained by bomb calorimetry was also made. The results show that Method B has the potentiality to evaluate the amount of combustion heat release of materials.
Preparation and Characterization of Electrosprayed Aerogel/Polytetrafluoroethylene Microporous Materials
Authors: Xiong Xiaoman , Venkataraman Mohanapriya , Yang Tao , Militký Jiří , Wiener Jakub
Published: December 2021

This paper presents the preparation of aerogel/polytetrafluoroethylene (PTFE) microporous materials via needleless electrospray technique, by using an aqueous dispersion of polytetrafluoroethylene as the basic spinning liquid. Different contents of aerogel powders were applied to the spinning liquid for electrospraying to investigate the effect on the structural characteristics and various properties of the materials. Cross-section, surface morphology, and particle size distribution of the electrosprayed materials were examined. Surface roughness, hydrophobicity, and thermal conductivity were evaluated and discussed. The results showed that the electrosprayed aerogel/PTFE layers were compact and disordered stacking structures composed of spherical particles with a rough surface. As the aerogel content increased, the electrosprayed materials demonstrated increased surface roughness and improved surface hydrophobicity with a contact angle up to 147.88°. In addition, the successful achievement of thermal conductivity as low as 0.024 (W m−1 K−1) indicated a superior ability of the prepared aerogel/PTFE composites to prevent heat transfer. This study contributes to the field of development of aerogel/PTFE composites via electrospray technique, providing enhanced final performance for potential use as thermal and moisture barriers in textiles or electronic devices.
Modes of Occurrence of Chromium and Their Thermal Stability in Low-Rank Coal Pyrolysis
Authors: Wang Xiaobing , Zhou Lingmei , Zhu Shuquan , Zheng Hao , Ma Yue , Liu Yuchen , Jia Chenxin , Zhou Changjiang , Bie Lijuan , Zhang Guanjun
Published: December 2021

Chromium (Cr) and the emission of its compounds into the environment have caused long-term environmental contamination. In this study, the modes of occurrence of Cr in low-rank coal and their thermal stability in pyrolysis were investigated by sequential chemical extraction (SCE), single-component samples (SCS) pyrolysis, and thermochemical equilibrium simulation. The results showed that organic matter, aluminosilicate, and carbonate were the dominant modes of occurrence of Cr in low-rank coal. The modes of occurrence and chlorine (Cl) content affected the volatilization of Cr in coal. The characteristic release temperature range of Cr bounded to aluminosilicate was >600 °C and 400–600 °C for Cr bounded to a disulfide. Cr bounded to organic matter almost released completely before 600 °C. Cl enhanced the volatility of Cr and reduced its release temperature in Cr bounded to aluminosilicate. The simulation showed the content of gas products was very low, mainly chlorides. While the content of solid products, sulfides, and oxides, was much higher than gas products, showing their high thermal stability. The sulfides and oxides in chars were closely related to the carbonate and aluminosilicate bound form of Cr. The results of the equilibrium simulation were consistent with the experimental results.
A Comprehensive Review of Recent Advancements in Cancer Immunotherapy and Generation of CAR T Cell by CRISPR-Cas9
Authors: Al Saber Md. , Biswas Partha , Dey Dipta , Kaium Md. Abu , Islam Md. Aminul , Tripty Miss Ismoth Ara , Rahman MD. Hasanur , Rahaman Tanjim Ishraq , Biswas Md. Yeaman , Paul Priyanka , Rahman Md. Ataur , Hasan Md. Nazmul , Kim Bonglee
Published: December 2021

The mechanisms involved in immune responses to cancer have been extensively studied for several decades, and considerable attention has been paid to harnessing the immune system’s therapeutic potential. Cancer immunotherapy has established itself as a promising new treatment option for a variety of cancer types. Various strategies including cancer vaccines, monoclonal antibodies (mAbs), adoptive T-cell cancer therapy and CAR T-cell therapy have gained prominence through immunotherapy. However, the full potential of cancer immunotherapy remains to be accomplished. In spite of having startling aspects, cancer immunotherapies have some difficulties including the inability to effectively target cancer antigens and the abnormalities in patients’ responses. With the advancement in technology, this system has changed the genome-based immunotherapy process in the human body including the generation of engineered T cells. Due to its high specificity, CRISPR-Cas9 has become a simple and flexible genome editing tool to target nearly any genomic locus. Recently, the CD19-mediated CAR T-cell (chimeric antigen receptor T cell) therapy has opened a new avenue for the treatment of human cancer, though low efficiency is a major drawback of this process. Thus, increasing the efficiency of the CAR T cell (engineered T cells that induce the chimeric antigen receptor) by using CRISPR-Cas9 technology could be a better weapon to fight against cancer. In this review, we have broadly focused on recent immunotherapeutic techniques against cancer and the use of CRISPR-Cas9 technology for the modification of the T cell, which can specifically recognize cancer cells and be used as immune-therapeutics against cancer.
Passive Freedom of Education: Educational Choice in Flanders and The Netherlands
Authors: Franken Leni , Bertram-Troost Gerdien
Published: December 2021

According to the ECHR, parents have the right to have their children educated in conformity with their own religious and philosophical convictions. In this contribution, we examine how this passive freedom of education is granted in the Belgian (Flemish) and Dutch education systems, which are both characterised by substantial funding of non-governmental (mainly Christian) schools. In order to do so, we will have a closer look at the diversity between denominational schools as well as to the diversity within these schools, with a particular focus on their school identity and their policy concerning Religious Education (RE). In addition, attention will be given to the organisation of RE classes in governmental schools, which is also considered a means to guarantee the passive freedom of education. Our analysis brings us to the conclusion that, in spite of a similar legal and financial framework, the Dutch constellation is currently best able to guarantee passive freedom of education for all in today’s secularised, pluralistic context.
Early Identification of the Maternal, Placental and Fetal Dialog in Gestational Diabetes and Its Prevention
Authors: Naeh Amir , Maor-Sagie Esther , Hallak Mordechai , Gabbay-Benziv Rinat
Published: December 2021

Gestational diabetes mellitus (GDM) complicates between 5 and 12% of pregnancies, with associated maternal, fetal, and neonatal complications. The ideal screening and diagnostic criteria to diagnose and treat GDM have not been established and, currently, diagnostic use with an oral glucose tolerance test occurs late in pregnancy and produces poor reproducibility. Therefore, in recent years, significant research has been undertaken to identify a first-trimester biomarker that can predict GDM later in pregnancy, enable early intervention, and reduce GDM-related adverse pregnancy outcomes. Possible biomarkers include glycemic markers (fasting glucose and hemoglobin A1c), adipocyte-derived markers (adiponectin and leptin), pregnancy-related markers (pregnancy-associated plasma protein-A and the placental growth factor), inflammatory markers (C-reactive protein and tumor necrosis factor-α), insulin resistance markers (sex hormone-binding globulin), and others. This review summarizes current data on first-trimester biomarkers, the advantages, and the limitations. Large multi-ethnic clinical trials and cost-effectiveness analyses are needed not only to build effective prediction models but also to validate their clinical use.
Comparison of Marine Gravity Measurements from Shipborne and Satellite Altimetry in the Arctic Ocean
Authors: Ling Zilong , Zhao Lihong , Zhang Tao , Zhai Guojun , Yang Fanlin
Published: December 2021

To understand the influence of sea ice on shipborne gravity measurements and the accuracy of the satellite-altimetry-derived gravity field in the Arctic Ocean, we compared shipborne gravity measurements with those obtained from satellite altimetric gravity measurements. The influence of sea ice on the shipborne gravity measurements was mainly concentrated in the 0–6 km wavelength range, and the standard deviation of the noise amplitudes was 2.62 mGal. Compared to ice-free regions, the accuracies in the region with floating ice were reduced by 13% for DTU21 and 6% for SV31. Due to the influence of sea ice, satellite altimetric gravity data lose significant information in the 9–12 km wavelength range. The coherence curve of the shipborne gravity with bathymetry was nearly the same as that of the satellite altimetric gravity. The satellite data contain nearly all of the significant information that is present in the shipborne data. The differences between the shipborne and satellite gravity data are small and can be used to study the crustal structure of the Arctic.
Dependence of the Hydration of the Lunar Surface on the Concentrations of TiO2, Plagioclase, and Spinel
Authors: Hess Marcel , Wöhler Christian , Berezhnoy Alexey A. , Bishop Janice L. , Shevchenko Vladislav V.
Published: December 2021

We investigate the interrelation between the hydration of the lunar regolith and the mineral composition of the surface of the Moon with respect to the concentrations of plagioclase, TiO2 (highly correlated with the oxide mineral ilmenite), and Mg-spinel. The spectral properties of lunar regions with a low concentration of plagioclase or a high concentration of TiO2 or Mg-spinel show a significant reduction in hydration at lunar midday compared to other compositions. This suggests that these oxide minerals contain less of the strongly bound OH component, which is not removed at lunar midday. The time-of-day-dependent variation of the 3 μm band depth is greater in TiO2-rich areas compared to other mare regions. The TiO2-rich regions therefore appear to have a strong tendency to adsorb solar wind-induced hydrogen into binding states of low energy that can more readily desorb and readsorb OH/H2O on a daily basis.
Flood Detection Using Multiple Chinese Satellite Datasets during 2020 China Summer Floods
Authors: Zhang Lianchong , Xia Junshi
Published: December 2021

Multiple source satellite datasets, including the Gaofen (GF) series and Zhuhai-1 hyperspectral, are provided to detect and monitor the floods. Considering the complexity of land cover changes within the flooded areas and the different characteristics of the multi-source remote sensing dataset, we proposed a new coarse-to-fine framework for detecting floods at a large scale. Firstly, the coarse results of the water body were generated by the binary segmentation of GF-3 SAR, the water indexes of GF-1/6 multispectral, and Zhuhai-1 hyperspectral images. Secondly, the fine results were achieved by the deep neural networks with noisy-label learning. More specifically, the Unet with the T-revision is adopted as the noisy label learning method. The results demonstrated the reliability and accuracy of water mapping retrieved by the noisy learning method. Finally, the differences in flooding patterns in different regions were also revealed. We presented examples of Poyang Lake to show the results of our framework. The rapid and robust flood monitoring method proposed is of great practical significance to the dynamic monitoring of flood situations and the quantitative assessment of flood disasters based on multiple Chinese satellite datasets.
LPIN: A Lightweight Progressive Inpainting Network for Improving the Robustness of Remote Sensing Images Scene Classification
Authors: An Weining , Zhang Xinqi , Wu Hang , Zhang Wenchang , Du Yaohua , Sun Jinggong
Published: December 2021

At present, the classification accuracy of high-resolution Remote Sensing Image Scene Classification (RSISC) has reached a quite high level on standard datasets. However, when coming to practical application, the intrinsic noise of satellite sensors and the disturbance of atmospheric environment often degrade real Remote Sensing (RS) images. It introduces defects to them, which affects the performance and reduces the robustness of RSISC methods. Moreover, due to the restriction of memory and power consumption, the methods also need a small number of parameters and fast computing speed to be implemented on small portable systems such as unmanned aerial vehicles. In this paper, a Lightweight Progressive Inpainting Network (LPIN) and a novel combined approach of LPIN and the existing RSISC methods are proposed to improve the robustness of RSISC tasks and satisfy the requirement of methods on portable systems. The defects in real RS images are inpainted by LPIN to provide a purified input for classification. With the combined approach, the classification accuracy on RS images with defects can be improved to the original level of those without defects. The LPIN is designed on the consideration of lightweight model. Measures are adopted to ensure a high gradient transmission efficiency while reducing the number of network parameters. Multiple loss functions are used to get reasonable and realistic inpainting results. Extensive tests of image inpainting of LPIN and classification tests with the combined approach on NWPU-RESISC45, UC Merced Land-Use and AID datasets are carried out which indicate that the LPIN achieves a state-of-the-art inpainting quality with less parameters and a faster inpainting speed. Furthermore, the combined approach keeps the comparable classification accuracy level on RS images with defects as that without defects, which will improve the robustness of high-resolution RSISC tasks.
Internal Tides and Their Intraseasonal Variability on the Continental Slope Northeast of Taiwan Island Derived from Mooring Observations and Satellite Data
Authors: Yin Yuqi , Liu Ze , Zhang Yuanzhi , Chu Qinqin , Liu Xihui , Hou Yijun , Zhao Xinhua
Published: December 2021

In this study, strong internal tides were observed on the continental slope northeast of Taiwan Island. Owing to the lack of long-term observations, these tides’ intraseasonal variability and the impact of the Kuroshio Current remain unclear. This study aimed to fill in the gaps using one-year continuous mooring observations, satellite data and analysis data. The horizontal kinetic energy (HKE) of semidiurnal internal tides showed that there was conspicuous energy from 100 days to 200 days, which was mainly attributed to the cross-term of HKE. The impact of the Kuroshio Current and mesoscale eddies on the HKEs were assessed: Cyclonic (anticyclonic) mesoscale eddies propagated from the open ocean, weakened (strengthened) the Kuroshio and shifted the Kuroshio onshore (offshore) northeast of Taiwan Island. The weakened (strengthened) Kuroshio increased (decreased) the shoreward velocity at the mooring site, and the onshore (offshore) Kuroshio migration increased (decreased) the northeastward velocity and enhanced (weakened) the HKEs of internal tides by modulating the tidal energy horizontal propagation. The weakened (strengthened) Kuroshio also resulted in gentler (steeper) isopycnals across the slope and enhanced (weakened) the HKEs of internal tides by influencing the interaction between ocean stratification and bottom topography.