bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Glucomannan Supplementation on Type II Diabetes Mellitus in Humans: A Meta-Analysis
Authors: Zhang Zhanzhi , Zhang Yu , Tao Xiaomei , Wang Yuying , Rao Benqiang , Shi Hanping
Published: January 2023

The hypoglycemic and lipid-lowering effects of glucomannan are widely known, and it is a potential effective treatment for type II diabetes. In this study, we evaluated the effects of glucomannan supplementation on blood-lipid-related indicators, blood-glucose-related indicators, blood pressure (BP), and body weight (BW) in patients suffering from type II diabetes. We searched databases including PubMed, Cochrane, the comprehensive biomedical research database (Embase), Web of Science, and China National Knowledge Infrastructure (CNKI) for literature on glucomannan and type II diabetes. Six randomized controlled trials (RCTs) were eligible (n = 440 participants) to be included in our analysis. Glucomannan not only reduced the total cholesterol (TC) (MD −0.38 [95% CI: −0.61, −0.15], p = 0.001) and low-density lipoprotein (LDL) levels (MD −0.35 [95% CI: −0.52, −0.17], p < 0.0001) compared with the control group, but also reduced the fasting blood glucose (FBG) (MD −1.08 [95% CI: −1.65, −0.50], p = 0.0002), 2 h postprandial blood glucose (P2hBG) (MD −1.92 [95% CI: −3.19, −0.65], p = 0.003), fasting insulin (FINS) (MD −1.59 [95% CI: −2.69, −0.50], p = 0.004), and serum fructosamine (SFRA) levels (SMD −1.19 [95% CI: −1.74, −0.64], p < 0.0001). Our analysis indicates that glucomannan is an effective nutritional intervention for type II diabetes.
Lifestyle Intervention Randomized Controlled Trial for Age-Related Macular Degeneration (AMD-Life): Study Design
Authors: de Koning-Backus Alexandra P. M. , Kiefte-de Jong Jessica C. , van Rooij Jeroen G. J. ,   AMD-Life Team, Uitterlinden André G. , Voortman Trudy G. , Meester-Smoor Magda A. , Klaver Caroline C. W.
Published: January 2023

Age-related macular degeneration (AMD) has a strong genetic basis, but environmental factors such as smoking and a healthy diet can decrease the genetic fate by up to 50%. Current guidelines for clinical management include recommendations for a healthy lifestyle and antioxidant supplementation. However, many ophthalmologists do not inform their patients of this AMD-beneficial lifestyle. An important reason is the lack of trust that transition of lifestyle will be feasible in persons of advanced age and lack of methodology to measure lifestyle or its biological effects. To address these issues, we set up the lifestyle intervention study AMD-Life. It aims to investigate whether personalized risk-profiling (including genetic testing) and/or additional coaching can motivate patients to change their lifestyle. It also explores which biomarkers best reflect lifestyle change beneficial for AMD. The first year is a three-arm, self-contained open-label randomized clinical trial. A total of 150 AMD patients aged 55–85 years were randomized into three arms: (A) merely standard recommendations; (B) A conditions plus personalized risk profiling based on genetics and lifestyle, (C) B conditions plus coaching. The second year tests sustainability of lifestyle changes without active intervention. AMD-Life can provide further insight into the relevance of these interventions for the clinical management of AMD.
Congruence between Physical Activity Patterns and Dietary Patterns Inferred from Analysis of Sex Differences in Lifestyle Behaviors of Late Adolescents from Poland: Cophylogenetic Approach
Authors: Domaradzki Jarosław
Published: January 2023

Global trends toward physical inactivity and the replacement of healthy dietary behaviors with unhealthy food consumption, particularly in late adolescence, were commonly observed. Thus, the aim of this work was twofold: (1) to examine congruence between PAPs and DPs by identifying sex differences in healthy and unhealthy behaviors and (2) to assess the explanation behind why certain participants were classified into clusters using BMI and fat mass covariates. Late adolescents (19–21 years of age, n = 107) were selected to study. PAPs and DPs were assessed with questionnaires (IPAQ, QEB) and body height, weight, and fat mass percentage were self-reported (the accuracy and reliability of self-reported data were empirically verified). A cophylogenetic approach with several clustering procedures (heatmaps and tanglegrams) was the way to solve the stated problems. Results showed that students achieved the minimal level of physical activity, lower than students from other regions in Poland, Czech Republic, or Germany. There was congruence between PAPs and DPs in some males’ and females’ behaviors. Significant similarities in sex patterns of behaviors were revealed (Mantel tests–PAPs: r = 0.70, p < 0.001; DPs: r = 0.84, p < 0.001). Similarities in PAPs were related to transportation cycling and school/work activities behaviors. Non-healthy triads in dietary behaviors were found: fast-food, sweetened beverages, and alcoholic drinks in the first, and sweets, fried meals, and yellow cheese in the second. One healthy diad was revealed: vegetables and fruits. Only females’ dietary behaviors were reflected in body mass index (F = 3.19, p = 0.049), percentage of body fat (F = 3.87, p = 0.027), and fat mass index (F = 3.83, p = 0.028). The usefulness of the cophenetic approach in patterns study was verified. Sex similarities and specific disparities could help preparing targeted tailored intervention programs that improve healthy habits in late adolescents. This is especially important in relation to females, who more strongly reflected dietary behaviors in body composition.
Twenty-Four-Hour Movement Behaviors, Fitness, and Adiposity in Preschoolers: A Network Analysis
Authors: Souza Alyce Rodrigues , Bandeira Paulo Felipe Ribeiro , da Silva Morgana Alves Correia , da Cunha Glacithane Lins , Pereira Daniel Fernandes , Martins Clarice
Published: January 2023

The present study aimed to verify the associations between compliance with the 24-h movement behavior recommendations, fitness, and adiposity markers in preschoolers, considering the non-linear nature of these associations. The sample was comprised of 253 preschoolers. Preschoolers were assessed for anthropometric data and wore an accelerometer for seven consecutive days. Screen time and sleep duration were parent-reported in a face-to-face interview. The PREFIT test battery was used to assess physical fitness components (lower-body strength, cardiorespiratory fitness, and speed/agility). Descriptive statistics were used to describe the variables, and a network analysis was conducted to assess the emerging pattern of associations between the variables. Preschoolers’ greatest compliance with recommendations was observed for physical activity, while the lowest compliance was observed for the screen time recommendation. Among children aged three years, only 2.2% complied with all recommendations; only 1.0% of the four-year-olds and 1.3% of the five-year-olds complied with all recommendations. The results of the network analysis and centrality measures emphasized that cardiorespiratory fitness (CRF) and compliance with movement behavior recommendations were the most critical variables to address in preschoolers, reinforcing the importance of intervention programs focused on intense activities.
HSPB5 Inhibition by NCI-41356 Reduces Experimental Lung Fibrosis by Blocking TGF-β1 Signaling
Authors: Tanguy Julie , Boutanquoi Pierre-Marie , Burgy Olivier , Dondaine Lucile , Beltramo Guillaume , Uyanik Burhan , Garrido Carmen , Bonniaud Philippe , Bellaye Pierre-Simon , Goirand Françoise
Published: January 2023

Idiopathic pulmonary fibrosis is a chronic, progressive and lethal disease of unknown etiology that ranks among the most frequent interstitial lung diseases. Idiopathic pulmonary fibrosis is characterized by dysregulated healing mechanisms that lead to the accumulation of large amounts of collagen in the lung tissue that disrupts the alveolar architecture. The two currently available treatments, nintedanib and pirfenidone, are only able to slow down the disease without being curative. We demonstrated in the past that HSPB5, a low molecular weight heat shock protein, was involved in the development of fibrosis and therefore was a potential therapeutic target. Here, we have explored whether NCI-41356, a chemical inhibitor of HSPB5, can limit the development of pulmonary fibrosis. In vivo, we used a mouse model in which fibrosis was induced by intratracheal injection of bleomycin. Mice were treated with NaCl or NCI-41356 (six times intravenously or three times intratracheally). Fibrosis was evaluated by collagen quantification, immunofluorescence and TGF-β gene expression. In vitro, we studied the specific role of NCI-41356 on the chaperone function of HSPB5 and the inhibitory properties of NCI-41356 on HSPB5 interaction with its partner SMAD4 during fibrosis. TGF-β1 signaling was evaluated by immunofluorescence and Western Blot in epithelial cells treated with TGF-β1 with or without NCI-41356. In vivo, NCI-41356 reduced the accumulation of collagen, the expression of TGF-β1 and pro-fibrotic markers (PAI-1, α-SMA). In vitro, NCI-41356 decreased the interaction between HSPB5 and SMAD4 and thus modulated the SMAD4 canonical nuclear translocation involved in TGF-β1 signaling, which may explain NCI-41356 anti-fibrotic properties. In this study, we determined that inhibition of HSPB5 by NCI-41356 could limit pulmonary fibrosis in mice by limiting the synthesis of collagen and pro-fibrotic markers. At the molecular level, this outcome may be explained by the effect of NCI-41356 inhibiting HSPB5/SMAD4 interaction, thus modulating SMAD4 and TGF-β1 signaling. Further investigations are needed to determine whether these results can be transposed to humans.
Antitumor Activity of Bioactive Compounds from Rapana venosa against Human Breast Cell Lines
Authors: Petrova Maria , Vlahova Zlatina , Schröder Maria , Todorova Jordana , Tzintzarov Alexander , Gospodinov Anastas , Velkova Lyudmila , Kaynarov Dimitar , Dolashki Aleksandar , Dolashka Pavlina , Ugrinova Iva
Published: January 2023

This study is the first report describing the promising antitumor activity of biologically active compounds isolated from the hemolymph of marine snail Rapana venosa—a fraction with Mw between 50 and 100 kDa and two structural subunits (RvH1 and RvH2), tested on a panel of human breast cell lines—six lines of different molecular subtypes of breast cancer MDA-MB-231, MDA-MB-468, BT-474, BT-549, SK-BR-3, and MCF-7 and the non-cancerous MCF-10A. The fraction with Mw 50–100 kDa (HRv 50–100) showed good antitumor activity manifested by a significant decrease in cell viability, altered morphology, autophagy, and p53 activation in treated cancer cells. An apparent synergistic effect was observed for the combination of HRv 50–100 with cis-platin for all tested cell lines. The combination of HRv 50–100 with cisplatin and/or tamoxifen is three times more effective compared to treatment with classical chemotherapeutics alone. The main proteins in the active fraction, with Mw at ~50 kDa, ~65 kDa, ~100 kDa, were identified by MALDI-MS, MS/MS analyses, and bioinformatics. Homology was established with known proteins with antitumor potential detected in different mollusc species: peroxidase-like protein, glycoproteins Aplysianin A, L-amino acid oxidase (LAAO), and the functional unit with Mw 50 kDa of RvH. Our study reveals new perspectives for application of HRv 50–100 as an antitumor agent used alone or as a booster in combination with different chemotherapies.
Pharmacological Treatment of Bipolar Depression: A Review of Observational Studies
Authors: Fellendorf Frederike T. , Caboni Edoardo , Paribello Pasquale , Pinna Martina , D’Aloja Ernesto , Carucci Sara , Pinna Federica , Reininghaus Eva Z. , Carpiniello Bernardo , Manchia Mirko
Published: January 2023

The persistence of depressive morbidity is frequent in bipolar disorder, and the pharmacological management of this symptomatology often lacks effectiveness. This systematic review aimed to summarize the results of the naturalistic observational studies on the pharmacological treatment of bipolar depression published through April 2022. The certainty of evidence was evaluated according to the GRADE approach. In sum, 16 studies on anticonvulsants, 20 on atypical antipsychotics, 2 on lithium, 28 on antidepressants, and 9 on other compounds were found. Lamotrigine, quetiapine, aripiprazole, and ketamine were the most investigated compounds. Overall, the results support the recommendations regarding the effectiveness of lamotrigine and quetiapine. In contrast to the current recommendations, aripiprazole was shown to be effective and generally well tolerated. Additionally, SSRIs were shown to be effective, but, since they were associated with a possibly higher switch risk, they should be used as an adjunctive therapy to mood stabilizers. Lithium was only studied in two trials but was shown to be effective, although the serum concentrations levels were not associated with clinical response. Finally, ketamine showed divergent response rates with a low certainty of evidence and, so far, unclear long-term effects. Heterogeneity in diagnosis, sample sizes, study designs, reporting of bias, and side effects limited the possibility of a head-to-head comparison.
Nanosized Drug Delivery Systems to Fight Tuberculosis
Authors: Bourguignon Tom , Godinez-Leon Jesus Alfredo , Gref Ruxandra
Published: January 2023

Tuberculosis (TB) is currently the second deadliest infectious disease. Existing antitubercular therapies are long, complex, and have severe side effects that result in low patient compliance. In this context, nanosized drug delivery systems (DDSs) have the potential to optimize the treatment’s efficiency while reducing its toxicity. Hundreds of publications illustrate the growing interest in this field. In this review, the main challenges related to the use of drug nanocarriers to fight TB are overviewed. Relevant publications regarding DDSs for the treatment of TB are classified according to the encapsulated drugs, from first-line to second-line drugs. The physicochemical and biological properties of the investigated formulations are listed. DDSs could simultaneously (i) optimize the therapy’s antibacterial effects; (ii) reduce the doses; (iii) reduce the posology; (iv) diminish the toxicity; and as a global result, (v) mitigate the emergence of resistant strains. Moreover, we highlight that host-directed therapy using nanoparticles (NPs) is a recent promising trend. Although the research on nanosized DDSs for TB treatment is expanding, clinical applications have yet to be developed. Most studies are only dedicated to the development of new formulations, without the in vivo proof of concept. In the near future, it is expected that NPs prepared by “green” scalable methods, with intrinsic antibacterial properties and capable of co-encapsulating synergistic drugs, may find applications to fight TB.
Ethyl Protocatechuate Encapsulation in Solid Lipid Nanoparticles: Assessment of Pharmacotechnical Parameters and Preliminary In Vitro Evaluation for Colorectal Cancer Treatment
Authors: Russo Stefano , Torrisi Cristina , Cardullo Nunzio , Muccilli Vera , La Mantia Alfonsina , Castelli Francesco , Acquaviva Rosaria , Sarpietro Maria Grazia
Published: January 2023

Colorectal cancer is one of the most diffused tumoral diseases. Since most medicaments employed for its treatment are debilitating, the use of naturally derived products, which can be effective against the mutated cells and, in addition, can reduce most inflammatory-related effects, could be extremely beneficial for the continued treatment of this disease. In this research, ethyl protocatechuate (PCAEE), a protocatechuic acid prodrug, was encapsulated in solid lipid nanoparticles (SLN) (prepared without and with Tween 80), which were characterized in terms of size, polydispersity index (PDI), zeta potential and thermotropic behavior. Encapsulation efficiency, release profile and interaction with a model of biomembrane were also assessed. The nanoparticles were tested in vitro on both healthy cells and on a model of tumoral cells. SLN prepared with Tween 80 was promising in terms of physicochemical properties (z-average of 190 nm, PDI 0.150 and zeta potential around −20 mV) and encapsulation efficiency (56%); they showed a desirable release profile, demonstrated an ability to penetrate and release the encapsulated PCAEE into a biomembrane model and were nontoxic on healthy cells. In addition, they caused a greater dose-dependent decrease in the viability of CaCo-2 cells than PCAEE alone. In conclusion, the formulation could be proposed for further studies to assess its suitability for the treatment of colorectal cancer.
3D-Printed Fast-Dissolving Oral Dosage Forms via Fused Deposition Modeling Based on Sugar Alcohol and Poly(Vinyl Alcohol)—Preparation, Drug Release Studies and In Vivo Oral Absorption
Authors: Ikeda Sorato , Kobayashi Masanori , Aoki Soken , Terukina Takayuki , Kanazawa Takanori , Kojima Hiroyuki , Kondo Hiromu
Published: January 2023

Three-dimensional printing technology holds marked promise for the pharmaceutical industry and is now under intense investigation. Most research is aimed at a greater efficiency in printing oral dosage forms using powder bed printing or fused deposition modeling (FDM). Oral dosage forms printed by FDM tend to be hard objects, which reduce the risk of cracking and chipping. However, one challenge in printing oral dosage forms via FDM is achieving rapid drug release, because the materials for FDM are basically thermoplastic polymers with slow drug release properties. In this study, we investigated printing a fast-dissolving oral dosage form by adding sugar alcohol to a poly(vinyl alcohol)-based formulation for FDM. Filaments which contain sugar alcohol were successfully prepared, and objects were printed with them as oral dosage forms by FDM. On drug release testing, a printed oral dosage form in a ring shape which contained 55% maltitol showed a more than 85% drug release in 15 min. In vivo oral absorption of this printed oral dosage form in dogs was comparable to that of a conventional fast-dissolving tablet. Of particular interest, the drug release profile and drug amount of the oral dosage forms can be easily controlled by a change in shape using 3D Computer Aided Design. These characteristics will encourage the prevalence of FDM by the pharmaceutical industry, and contribute to the promotion of personalized medicine.
Dendrimers as Modifiers of Inorganic Nanoparticles for Therapeutic Delivery in Cancer
Authors: Zenze Mkhuseli , Daniels Aliscia , Singh Moganavelli
Published: January 2023

The formulation of nanoscale systems with well-defined sizes and shapes is of great interest in applications such as drug and gene delivery, diagnostics and imaging. Dendrimers are polymers that have attracted interest due to their size, shape, branching length, amine density, and surface functionalities. These unique characteristics of dendrimers set them apart from other polymers, their ability to modify nanoparticles (NPs) for biomedical applications. Dendrimers are spherical with multiple layers over their central core, each representing a generation. Their amphiphilic nature and hollow structure allow for the incorporation of multiple drugs or genes, in addition to enabling easy surface modification with cellular receptor-targeting moieties to ensure site-specific delivery of therapeutics. Dendrimers are employed in chemotherapeutic applications for the delivery of anticancer drugs. There are many inorganic NPs currently being investigated for cancer therapy, each with their own unique biological, chemical, and physical properties. To favor biomedical applications, inorganic NPs require suitable polymers to ensure stability, biodegradability and target specificity. The success of dendrimers is dependent on their unique structure, good bioavailability and stability. In this review, we describe the properties of dendrimers and their use as modifiers of inorganic NPs for enhanced therapeutic delivery. Herein, we review the significant developments in this area from 2015 to 2022. Databases including Web of Science, Scopus, Google Scholar, Science Direct, BioMed Central (BMC), and PubMed were searched for articles using dendrimers, inorganic nanoparticles and cancer as keywords.
Current Status of Polysaccharides-Based Drug Delivery Systems for Nervous Tissue Injuries Repair
Authors: Valentino Caterina , Vigani Barbara , Sandri Giuseppina , Ferrari Franca , Rossi Silvia
Published: January 2023

Neurological disorders affecting both CNS and PNS still represent one of the most critical and challenging pathologies, therefore many researchers have been focusing on this field in recent decades. Spinal cord injury (SCI) and peripheral nerve injury (PNI) are severely disabling diseases leading to dramatic and, in most cases, irreversible sensory, motor, and autonomic impairments. The challenging pathophysiologic consequences involved in SCI and PNI are demanding the development of more effective therapeutic strategies since, as yet, a therapeutic strategy that can effectively lead to a complete recovery from such pathologies is not available. Drug delivery systems (DDSs) based on polysaccharides have been receiving more and more attention for a wide range of applications, due to their outstanding physical-chemical properties. This review aims at providing an overview of the most studied polysaccharides used for the development of DDSs intended for the repair and regeneration of a damaged nervous system, with particular attention to spinal cord and peripheral nerve injury treatments. In particular, DDSs based on chitosan and their association with alginate, dextran, agarose, cellulose, and gellan were thoroughly revised.
Nitrogen Fertilizer Type and Genotype as Drivers of P Acquisition and Rhizosphere Microbiota Assembly in Juvenile Maize Plants
Authors: Mang Melissa , Maywald Niels Julian , Li Xuelian , Ludewig Uwe , Francioli Davide
Published: January 2023

Phosphorus (P) is an essential nutrient for plant growth and development, as well as an important factor limiting sustainable maize production. Targeted nitrogen (N) fertilization in the form of ammonium has been shown to positively affect Pi uptake under P-deficient conditions compared to nitrate. Nevertheless, its profound effects on root traits, P uptake, and soil microbial composition are still largely unknown. In this study, two maize genotypes F160 and F7 with different P sensitivity were used to investigate phosphorus-related root traits such as root hair length, root diameter, AMF association, and multiple P efficiencies under P limitation when fertilized either with ammonium or nitrate. Ammonium application improved phosphorous acquisition efficiency in the F7 genotype but not in F160, suggesting that the genotype plays an important role in how a particular N form affects P uptake in maize. Additionally, metabarcoding data showed that young maize roots were able to promote distinct microbial taxa, such as arbuscular mycorrhizal fungi, when fertilized with ammonium. Overall, the results suggest that the form of chemical nitrogen fertilizer can be instrumental in selecting beneficial microbial communities associated with phosphorus uptake and maize plant fitness.
Biotechnological Approach to Increase Oxyresveratrol Production in Mulberry In Vitro Plants under Elicitation
Authors: Sabater-Jara Ana Belén , Almagro Lorena , Nicolás Sánchez Isabel , Pedreño María Ángeles
Published: January 2023

Morus alba L. is used for a range of therapeutic purposes in Asian traditional medicine, and its extracts are reported to be effective against lipidemia, diabetes, and obesity, as well as being hepatoprotective and tyrosinase-inhibitory. They are also included in cosmetic products as anti-aging and skin-whitening agents. Stilbenes, the major bioactive compounds found in M. alba, have received renewed attention recently because of their putative activity against COVID-19. In this study M. alba plants were established in vitro, and the effect of elicitation on plant growth and stilbene accumulation, specifically oxyresveratrol and trans-resveratrol, was investigated. Different concentrations of the elicitors including methyl jasmonate and cyclodextrins were applied, and stilbene levels were determined in leaves, roots, and the culture medium. Elicitation of the M. alba plants with 5 mM cyclodextrins, alone or in combination with 10 µM methyl jasmonate, significantly increased the total phenolic content in the culture medium and leaves after 7 days of treatment. The higher total phenolic content in the roots of control plants and those treated only with methyl jasmonate indicated that cyclodextrins promoted metabolite release to the culture medium. Notably, the cyclodextrin-treated plants with the highest levels of oxy- and trans-resveratrol also had the highest total phenolic content and antioxidant capacity. These results indicate that elicited M. alba in vitro plants constitute a promising alternative source of bioactive stilbenes to supply pharmaceutical and cosmeceutical industries.
Contributing Factors of Dielectric Properties for Polymer Matrix Composites
Authors: Wang Quan , Che Junbo , Wu Weifei , Hu Zhendong , Liu Xueqing , Ren Tianli , Chen Yuwei , Zhang Jianming
Published: January 2023

Due to the trend of multi-function, integration, and miniaturization of electronics, traditional dielectric materials are difficult to satisfy new requirements, such as balanced dielectric properties and good designability. Therefore, high dielectric polymer composites have attracted wide attention due to their outstanding processibility, good designability, and dielectric properties. A number of polymer composites are employed in capacitors and sensors. All these applications are directly affected by the composite’s dielectric properties, which are highly depended on the compositions and internal structure design, including the polymer matrix, fillers, structural design, etc. In this review, the influences of matrix, fillers, and filler arrangement on dielectric properties are systematically and comprehensively summarized and the regulation strategies of dielectric loss are introduced as well. Finally, the challenges and prospects of high dielectric polymer composites are proposed.
Carrier Variety Used in Immobilization of His6-OPH Extends Its Application Areas
Authors: Efremenko Elena , Lyagin Ilya , Aslanli Aysel , Stepanov Nikolay , Maslova Olga , Senko Olga
Published: January 2023

Organophosphorus hydrolase, containing a genetically introduced hexahistidine sequence (His6-OPH), attracts the attention of researchers by its promiscuous activity in hydrolytic reactions with various substrates, such as organophosphorus pesticides and chemical warfare agents, mycotoxins, and N-acyl homoserine lactones. The application of various carrier materials (metal-organic frameworks, polypeptides, bacterial cellulose, polyhydroxybutyrate, succinylated gelatin, etc.) for the immobilization and stabilization of His6-OPH by various methods, enables creation of biocatalysts with various properties and potential uses, in particular, as antidotes, recognition elements of biosensors, in fibers with chemical and biological protection, dressings with antimicrobial properties, highly porous sorbents for the degradation of toxicants, including in flow systems, etc. The use of computer modeling methods in the development of immobilized His6-OPH samples provides in silico prediction of emerging interactions between the enzyme and immobilizing polymer, which may have negative effects on the catalytic properties of the enzyme, and selection of the best options for experiments in vitro and in vivo. This review is aimed at analysis of known developments with immobilized His6-OPH, which allows to recognize existing recent trends in this field of research, as well as to identify the reasons limiting the use of a number of polymer molecules for the immobilization of this enzyme.
Dielectric Characterization of Core-Shell Structured Poly(vinylidene fluoride)-grafted-BaTiO3 Nanocomposites
Authors: Bouharras Fatima Ezzahra , Labardi Massimiliano , Tombari Elpidio , Capaccioli Simone , Raihane Mustapha , Améduri Bruno
Published: January 2023

Dielectric properties of poly(vinylidene fluoride)-grafted-BaTiO3 (PVDF-g-BT) core-shell structured nanocomposites obtained from Reversible Addition Fragmentation chain Transfer (RAFT) polymerization of VDF were investigated by Broadband Dielectric Spectroscopy (BDS). The dielectric constant increased along with the BT content, about +50% by addition of 15 vol% of BT, which was around 40% more than expected from predictions using the usual dielectric modeling methods for composite materials, to be ascribed to the effect of the interfacial core-shell structure. The known dielectric relaxations for PVDF were observed for the neat polymer as well as for its nanocomposites, not affected by the presence of nanoparticles. A relaxation process at higher temperatures was found, due to interfacial polarization at the amorphous-crystalline interface, due to the high crystallinity of materials produced by RAFT. Isochronal BDS spectra were exploited to detect the primary relaxation of the amorphous fraction. Thermal analysis demonstrated a very broad endotherm at temperatures much lower than the usual melting peaks, possibly due to the ungrafted fraction of the polymer that is more easily removable by repeated washing of the pristine material with acetone.
Electrospun Co Nanoparticles@PVDF-HFP Nanofibers as Efficient Catalyst for Dehydrogenation of Sodium Borohydride
Authors: Abutaleb Ahmed , Maafa Ibrahim M. , Zouli Nasser , Yousef Ayman , El-Halwany M. M.
Published: January 2023

Metallic Co NPs@poly(vinylidene fluoride-co- hexafluoropropylene) nanofibers (PVFH NFs) were successfully synthesized with the help of electrospinning and in situ reduction of Co2+ ions onto the surface of PVFH membrane. Synthesis of PVFH NFs containing 10, 20, 30, and 40 wt% of cobalt acetate tetrahydrate was achieved. Physiochemical techniques were used to confirm the formation of metallic Co@PVFH NFs. High catalytic activity of Co@PVFH NFs in the dehydrogenation sodium borohydride (SBH) was demonstrated. The formulation with 40 wt% Co proved to have the greatest performance in comparison to the others. Using 1 mmol of SBH and 100 mg of Co@PVFH NFs, 110 mL of H2 was produced in 19 min at a temperature of 25 °C, but only 56, 73, and 89 mL were produced using 10, 20, and 30 wt% Co, respectively. With the rise of catalyst concentration and reaction temperature, the amount of hydrogen generated increased. By raising the temperature from 25 to 55 °C, the activation energy was lowered to be 35.21 kJ mol−1 and the yield of H2 generation was raised to 100% in only 6 min. The kinetic study demonstrated that the reaction was pseudo-first order in terms of the amount of catalyst utilized and pseudo-zero order in terms of the SBH concentration. In addition, after six cycles of hydrolysis, the catalyst showed outstanding stability. The suggested catalyst has potential applications in H2 generation through hydrolysis of sodium borohydride due to its high catalytic activity and flexibility of recycling.
Baicalin-Loaded Lipid–Polymer Hybrid Nanoparticles Inhibiting the Proliferation of Human Colon Cancer: Pharmacokinetics and In Vivo Evaluation
Authors: Riadi Yassine , Afzal Obaid , Geesi Mohammed H. , Almalki Waleed H. , Singh Tanuja
Published: January 2023

This research work is focused on pharmacokinetic and biochemical experiments to assess baicalin-loaded lipid–polymer hybrid nanoparticles (LPHNPs) with colon-targeting specificity. The nanoprecipitation method was used to develop the LPHNPs, and the characterized formulation revealed the 184.3 nm particle size, PDI of 0.177, spherical shape, and zeta potential of −19.8 mV. The baicalin LPHNPs are said to be poorly absorbed in the stomach and small intestine, and in vitro drug release tests have shown that the drug is released mostly in the caecal fluid. Additionally, the LPHNPs showed stability and nonsignificant drug loss at 25 °C for 3 months. The least viable population of baicalin-loaded LPHNPs was detected at a lower IC50 value after 48 h, and no cytotoxicity was observed by blank suspension and blank LPHNPs up to the concentration of 100 µg/mL. Apart from this, the pharmacokinetics study showed that baicalin from LPHNPs is much less absorbed and least available in the blood plasma and maximum available in the colon. Concurrently, organ distribution studies demonstrated that baicalin-loaded LPHNPs were distributed more widely in the colon compared to baicalin suspension. Moreover, baicalin-loaded LPHNPs were found to be superior to a baicalin suspension in reducing elevated liver enzyme levels. In a nutshell, baicalin-loaded LPHNPs show superior efficacy and can be maximally localized into the colon rectal cancer along with systemic availability of the drug.
Three-Dimensional Modeling of Spun-Bonded Nonwoven Meso-Structures
Authors: Ke Zhenxia , Yu Lingjie , Wang Guanlin , Sun Runjun , Zhu Mengqiu , Dong Hanrui , Xu Yiqin , Ren Mengyue , Fu Sida , Zhi Chao
Published: January 2023

As a type of fiber system, nonwoven fabric is ideal for solid–liquid separation and air filtration. With the wide application of nonwoven filter materials, it is crucial to explore the complex relationship between its meso structure and filtration performance. In this paper, we proposed a novel method for constructing the real meso-structure of spun-bonded nonwoven fabric using computer image processing technology based on the idea of a “point-line-body”. Furthermore, the finite element method was adopted to predict filtration efficiencies based on the built 3D model. To verify the effectiveness of the constructed meso-structure and simulation model, filtration experiments were carried out on the fabric samples under different pollution particle sizes and inlet velocities. The experimental results show that the trends observed in the simulation results are consistent with those of the experimental results, with a relative error smaller than 10% for any individual datum.
Characterization and Gel Properties of Low-Molecular-Weight Carrageenans Prepared by Photocatalytic Degradation
Authors: Song Chen , You Ying , Wen Chengrong , Fu Yinghuan , Yang Jingfeng , Zhao Jun , Song Shuang
Published: January 2023

Low-molecular-weight carrageenan has attracted great interest because it shows advantages in solubility, absorption efficiency, and bioavailability compared to original carrageenan. However more environment-friendly and efficient methods to prepare low-molecular-weight carrageenan are still in great need. In the present study, a photocatalytic degradation method with only TiO2 has been developed and it could decrease the average molecular weight of κ-carrageenan to 4 kDa within 6 h. The comparison of the chemical compositions of the degradation products with those of carrageenan by FT-IR, NMR, etc., indicates no obvious removement of sulfate group, which is essential for bioactivities. Then 20 carrageenan oligosaccharides in the degradation products were identified by HPLC-MSn, and 75% of them possessed AnGal or its decarbonylated derivative at their reducing end, indicating that photocatalysis is preferential to break the glycosidic bond of AnGal. Moreover, the analysis results rheology and Cryo-SEM demonstrated that the gel property decreased gradually. Therefore, the present study demonstrated that the photocatalytic method with TiO2 as the only catalyst has the potential to prepare low-molecular-weight carrageenan with high sulfation degree and low viscosity, and it also proposed the degradation rules after characterizing the degradation products. Thus, the present study provides an effective green method for the degradation of carrageenan.
Molecular-Level Insight of CP52/NBR Damping Composites through a Combination of Molecular Dynamics Simulation and Experimental Method
Authors: Wang Qingxin , Li Yu , Li Shuaijie , Sun Zhaoyi , Wang Guorong
Published: January 2023

To enhance the damping properties of nitrile butadiene rubber (NBR), the elastomer used was blended with chlorinated paraffin 52 (CP52) to prepare NBR/CP52 composites. The results showed that CP52 could significantly enhance the damping properties of NBR and shift the glass transition temperature (Tg) to lower temperatures. Molecular dynamics models of the CP52/NBR system were established, and the damping properties of the CP52-reinforced NBR were investigated using molecular dynamics (MD) simulations. Through the combination of MD simulations and the experimental results, the essential mechanism of the enhanced damping properties of the NBR was methodically expatiated and was ascribed to the Cl-CP-H····NC-NBR (type I) and CP-Cl····H-NBR-CN (type II) analogous hydrogen bonds formed between NBR and CP52. The higher the CP52 content, the higher the analogous hydrogen bond concentration, and the better the damping properties of the CP52/NBR composites. The experimental results were very consistent with the MD simulation results, meaning that the combination method can provide a new means to optimize the design of damping materials and broaden the application range of small polar molecules in the damping modification of polar rubber materials.
Preparation and Process Parameter Optimization of Continuous Carbon Fiber-Reinforced Polycarbonate Prepreg Filament
Authors: Chen Xun , Wang Yesong , Liu Manxian , Qu Sheng , Zhang Qing , Chen Shuguang
Published: January 2023

Continuous fiber-reinforced composite 3D printing (CFRC 3DP) has become a hot topic of interest for many experts and scholars. Continuous fiber-reinforced prepreg filament (CFRPF) for printing needs to be prepared in advance. In this paper, on the basis of the resin fusion impregnation theory, a fabrication device was designed for continuous carbon fiber-reinforced polycarbonate prepreg filament (CCFRPF). Then, according to the orthogonal test and the TOPSIS entropy weight optimization theory, the optimization method for CFRPF/PC preparation process parameters was proposed, and the relationship between the preparation process parameters and the performance indexes was discussed. The results show that when preparing CCFRPF/PC, the weight of diameter performance index is the largest, about 0.75. The optimal combination of process parameters for CCFRPF/PC is, respectively, 285 °C for the outlet mold temperature, 305 °C for the impregnation mold temperature, and 1 m/min for the winding speed. In this case, the diameter, roundness, minimum curvature radius and tensile strength of 0.375 mm, 29.4 μm, 9.775 mm and 1298 MPa were achieved, respectively.
Research on the Sound Insulation Performance of Composite Rubber Reinforced with Hollow Glass Microsphere Based on Acoustic Finite Element Simulation
Authors: Yang Xiaocui , Tang Shuai , Shen Xinmin , Peng Wenqiang
Published: January 2023

The composite rubber reinforced with hollow glass microsphere (HGM) was a promising composite material for noise reduction, and its sound insulation mechanism was studied based on an acoustic finite element simulation to gain the appropriate parameter with certain constraint conditions. The built simulation model included the air domain, polymer domain and inorganic particles domain. The sound insulation mechanism of the composite material was investigated through distributions of the sound pressure and sound pressure level. The influences of the parameters on the sound transmission loss (STL) were researched one by one, such as the densities of the composite rubber and HGM, the acoustic velocities in the polymer and inorganic particle, the frequency of the incident wave, the thickness of the sound insulator, and the diameter, volume ratio and hollow ratio of the HGM. The weighted STL with the 1/3 octave band was treated as the evaluation criterion to compare the sound insulation property with the various parameters. For the limited thicknesses of 1 mm, 2 mm, 3 mm and 4 mm, the corresponding optimal weighted STL of the composite material reached 14.02 dB, 19.88 dB, 22.838 dB and 25.27 dB with the selected parameters, which exhibited an excellent sound insulation performance and could promote the practical applications of the proposed composite rubber reinforced with HGM.
A New Semi-Analytical MC Model for Oceanic LIDAR Inelastic Signals
Authors: Chen Su , Chen Peng , Ding Lei , Pan Delu
Published: January 2023

The design and processing algorithm of oceanic LIDAR requires an effective lidar simulator. Currently, most simulation methods for lidar signal propagation in seawater use elastic scattering. In this study, a new semi-analytical Monte Carlo (MC) model for oceanic lidar inelastic signals is developed to investigate chlorophyll fluorescence and Raman scattering in seawater. We also used this model to simulate the echo signal of high spectral resolution lidar (HSRL) in the particulate and water molecular channels. Using this model, the effects of chlorophyll concentration, multiple scattering, receiving field of view (FOV), scattering phase function (SPF), receiver full width at half maximum (FWHM) and inhomogeneous seawater were investigated. The feasibility and effectiveness of the model were verified by the lidar equation under small and large FOVs. The results showed that chlorophyll concentration and vertical structure and multiple scattering have considerable and integrated effects on echo signals, which could provide a reference for the design of oceanic fluorescence and HSRL lidar systems and contribute to the development of processing algorithms.
Imaging Simulation Method for Novel Rotating Synthetic Aperture System Based on Conditional Convolutional Neural Network
Authors: Sun Yu , Zhi Xiyang , Jiang Shikai , Gong Jinnan , Shi Tianjun , Wang Nan
Published: January 2023

The novel rotating synthetic aperture (RSA) is a new optical imaging system that uses the method of rotating the rectangular primary mirror for dynamic imaging. It has the advantage of being lightweight, with no need for splicing and real-time surface shape maintenance on orbit. The novel imaging method leads to complex image quality degradation characteristics. Therefore, it is vital to use the image quality improvement method to restore and improve the image quality to meet the application requirements. For the RSA system, a new system that has not been applied in orbit, it is difficult to construct suitable large datasets. Therefore, it is necessary to study and establish the dynamic imaging characteristic model of the RSA system, and on this basis provide data support for the corresponding image super resolution and restoration method through simulation. In this paper, we first analyze the imaging characteristics and mathematically model the rectangular rotary pupil of the RSA system. On this basis, combined with the analysis of the physical interpretation of the blur kernel, we find that the optimal blur kernel is not the point spread function (PSF) of the imaging system. Therefore, the simulation method of convolving the input image directly with the PSF is flawed. Furthermore, the weights of a convolutional neural network (CNN) are the same for each input. This means that the normal convolutional layer is not only difficult to accurately estimate the time-varying blur kernel, but also difficult to adapt to the change in the length–width ratio of the primary mirror. To that end, we propose a blur kernel estimation conditional convolutional neural network (CCNN) that is equivalent to multiple normal CNNs. We extend the CNN to a conditional model by taking an encoding as an additional input and using conditionally parameterized convolutions instead of normal convolutions. The CCNN can simulate the imaging characteristics of the rectangular pupil with different length–width ratios and different rotation angles in a controllable manner. The results of semi-physical experiments show that the proposed simulation method achieves a satisfactory performance, which can provide data and theoretical support for the image restoration and super-resolution method of the RSA system.
A Spectral–Spatial Method for Mapping Fire Severity Using Morphological Attribute Profiles
Authors: Ren Xiaoyang , Yu Xin , Wang Yi
Published: January 2023

Fast and accurate fire severity mapping can provide an essential resource for fire management and studying fire-related ecological and climate change. Currently, mainstream fire severity mapping approaches are based only on pixel-wise spectral features. However, the landscape pattern of fire severity originates from variations in spatial dependence, which should be described by spatial features combined with spectral features. In this paper, we propose a morphological attribute profiles-based spectral–spatial approach, named Burn Attribute Profiles (BAP), to improve fire severity classification and mapping accuracy. Specifically, the BAP method uses principal component transformation and attributes with automatically determined thresholds to extract spatial features, which are integrated with spectral features to form spectral–spatial features for fire severity. We systematically tested and compared the BAP-based spectral–spatial features and spectral index features in the extremely randomized trees machine learning framework. Sentinel-2 imagery was used for seven fires in the Mediterranean region, while Landsat-8 imagery was used for another seven fires in the northwestern continental United States region. The results showed that, except for 2 fires (overall accuracy (OA) for EMSR213_P: 59.6%, EL: 59.5%), BAP performed well for the other 12 fires (OA for the 2 fires: 60–70%, 6 fires: 70–80%, 4 fires: >80%). Furthermore, compared with the spectral indices-based method, the BAP method showed OA improvement in all 14 fires (OA improvement in Mediterranean: 0.2–14.3%, US: 4.7–12.9%). Recall and Precision were also improved for each fire severity level in most fire events. Moreover, the BAP method improved the “salt-and-pepper” phenomenon in the homogeneous area, where the results are visually closest to the reference data. The above results suggest that the spectral–spatial method based on morphological attribute profiles can map fire severity more accurately.
Experimental Assessment of Common Crucial Factors That Affect LoRaWAN Performance on Suburban and Rural Area Deployments
Authors: Fragkopoulos Markos , Panagiotakis Spyridon , Kostakis Michail , Markakis Evangelos K. , Astyrakakis Nikolaos , Malamos Athanasios
Published: January 2023

LoRaWAN networks might be a technology that could facilitate extreme energy-efficient operation while offering great capacity for suburban and rural area deployment, but this can be a challenging task for a network administrator. Constraints that deform the trade-off triangle of coverage, scalability and energy efficiency need to be overcome. The scope of this study is to review the limitations of the LoRaWAN protocol in order to summarize and assess the crucial factors that affect communication performance, related to data rate allocation, bidirectional traffic and radio spectrum utilization. Based on the literature, these factors correspond mostly to configurable payload transmission parameters, including transmission interval, data rate allocation, requirement for acknowledgements and retransmission. In this work, with simulation experiments, we find that collision occurrences greatly affect channel occupancy. In particular, it was evaluated that collision occurrence is increasingly affected by transmission intervals, which have the most significant negative impact on packet delivery rate (PDR). We then validated that clustering of end nodes in the vicinity of a gateway, taking into account distance and transmission settings, can improve network scalability. This can assure distribution of the total transmission time to end nodes with respect to application-related QoS requirements. Following this clustering approach, we achieved a PDR greater than 0.90 in a simulation setting with 6000 end nodes in a 10 km coverage.