bluuebunny/crossref_metadata_2025 huggingface dataset:
Sanxan–Protein Complex Particles for Stabilization of Pickering Emulsions: Improving Emulsification Properties
Authors: Wang Qianqian , Wang Xinyu , Qi Xiaoxi , Liu Libo , Zhang Guofang , Du Peng , Li Chun
Published: November 2024

Sanxan (SAN) is a novel microbial polysaccharide that is both safe and edible and represents a promising new source of food resources. It exhibits gelling properties and certain emulsifying properties. To date, there have been few studies published on the enhancement of protein emulsification by sanxan. In this study, three widely used proteins were used: casein (CS), pea protein isolate (PPI), and soy protein isolate (SPI). SAN–protein composite particles were prepared by non-covalent interactions to evaluate the availability of SAN in Pickering emulsions. The effect of SAN on the ability of the complexes to stabilize the emulsion was investigated by measuring and characterizing the physicochemical properties of three SAN–protein complexes. Fourier transform infrared (FTIR) and fluorescence spectroscopy analyses showed that SAN was able to bind to three proteins to form complexes. All three complexes formed by SAN with SPI, PPI and CS had good emulsification properties, with PPI-SAN being the best. Storage results showed better stability of the composite particle-stabilized emulsion. These results indicate that the complexation of SAN with proteins improves the emulsification of proteins and increases the stability of Pickering emulsions. The findings of this study provide valuable information for the utilization of SAN in emulsions.
Assessment of Tail-Cutting in Frozen Albacore (Thunnus alalunga) Through Ultrasound Inspection and Chemical Analysis
Authors: Yagi Masafumi , Sakai Akira , Yasutomi Suguru , Suzuki Kanata , Kashikura Hiroki , Goto Keiichi
Published: November 2024

Fat content is the main criterion for evaluating albacore quality. However, no reports exist on the accuracy of the tail-cutting method, a method used to assess the fat content of albacore. Here, we evaluated this method by comparing it with chemical analysis and ultrasound inspection. We measured the actual fat content in albacore using chemical analysis and compared the results with those obtained using the tail-cutting method. Significant discrepancies (99% CI, t-test) were observed in fat content among the tail-cutting samples. Using chemical analysis as the ground truth, the accuracy of tail-cutting from two different companies was 70.0% for company A and 51.9% for company B. An ultrasound inspection revealed that a higher fat content reduced the amplitude of ultrasound signals with statistical significance (99% CI, t-test). Finally, machine learning algorithms were used to enforce the ultrasound inspection. The best combination of ultrasound inspection and a machine learning algorithm achieved an 84.2% accuracy for selecting fat-rich albacore, which is better than tail-cutting (73.6%). Our findings suggested that ultrasound inspection could be a valuable and non-destructive method for estimating the fat content of albacore, achieving better accuracy than the traditional tail-cutting method.
Spectral Fingerprinting of Tencha Processing: Optimising the Detection of Total Free Amino Acid Content in Processing Lines by Hyperspectral Analysis
Authors: He Qinghai , Guo Yihang , Li Xiaoli , He Yong , Lin Zhi , Zeng Hui
Published: November 2024

The quality and flavor of tea leaves are significantly influenced by chemical composition, with the content of free amino acids serving as a key indicator for assessing the quality of Tencha. Accurately and quickly measuring free amino acids during tea processing is crucial for monitoring and optimizing production processes. However, traditional chemical analysis methods are often time-consuming and costly, limiting their application in real-time quality control. Hyperspectral imaging (HSI) has shown significant effectiveness as a component detection tool in various agricultural applications. This study employs VNIR-HSI combined with machine learning algorithms to develop a model for visualizing the total free amino acid content in Tencha samples that have undergone different processing steps on the production line. Four pretreating methods were employed to preprocess the spectra, and partial least squares regression (PLSR) and least squares support vector machine regression (LS–SVR) models were established from the perspectives of individual processes and the entire process. Combining competitive adaptive reweighted sampling (CARS) and variable iterative space shrinkage approach (VISSA) methods for characteristic band selection, specific bands were chosen to predict the amino acid content. By comparing modeling evaluation indicators for each model, the optimal model was identified: the overall model CT+CARS+PLSR, with predictive indicators Rc2 = 0.9885, Rp2 = 0.9566, RMSEC = 0.0956, RMSEP = 0.1749, RPD = 4.8021, enabling the visualization of total free amino acid content in processed Tencha leaves. Here, we establish a benchmark for machine learning-based HSI, integrating this technology into the tea processing workflow to provide a real-time decision support tool for quality control, offering a novel method for the rapid and accurate prediction of free amino acids during tea processing. This achievement not only provides a scientific basis for the tea processing sector but also opens new avenues for the application of hyperspectral imaging technology in food science.
Bacteriocin Mining in Lactiplantibacillus pentosus PCZ4 with Broad-Spectrum Antibacterial Activity and Its Biopreservative Effects on Snakehead Fish
Authors: Du Hechao , Li Siyu , Yao Hongliang , Wang Nannan , Zhao Ruiqiu , Meng Fanqiang
Published: November 2024

Some lactic acid bacteria (LAB) produce antibacterial substances such as bacteriocins, making them promising candidates for food preservation. In our study, Lactiplantibacillus pentosus PCZ4—a strain with broad-spectrum antibacterial activity—was isolated from traditional fermented kimchi in Sichuan. Whole-genome sequencing of PCZ4 revealed one chromosome and three plasmids. Through BAGEL4 mining, classes IIa and IIb bacteriocin plantaricin S were identified. Additionally, two new antibacterial peptides, Bac1109 and Bac2485, were predicted from scratch by limiting open reading frames. Furthermore, during refrigerated storage of snakehead fish, PCZ4 crude extract reduced the total bacterial count, slowed the increase in TVB-N and pH values, improved the sensory quality of the snakehead, and extended its shelf life by 2 days. Meanwhile, PCZ4 effectively inhibited the growth of artificially contaminated Aeromonas hydrophila in snakehead fish. These findings indicate that Lp. pentosus PCZ4 can produce multiple antibacterial substances with strong potential for food preservation applications.
Green Tea Epigallocatechin 3-Gallate Reduced Platelet Aggregation and Improved Anticoagulant Proteins in Patients with Transfusion-Dependent β-Thalassemia: A Randomized Placebo-Controlled Clinical Trial
Authors: Petiwathayakorn Touchwin , Hantrakool Sasinee , Settakorn Kornvipa , Hutachok Nuntouchaporn , Tantiworawit Adisak , Chalortham Nopphadol , Koonyosying Pimpisid , Srichairatanakool Somdet
Published: November 2024

Patients with transfusion-dependent β-thalassemia (TDT) with iron overload have been linked to hypercoagulability and increased platelet (PLT) activation that causes thrombosis. Green tea extract (GTE) rich in epigallocatechin-3-gallate (EGCG) exerts iron-chelating and antithrombotic properties. The study aimed to assess the effects of GTE treatment on plasma coagulation state and PLT function in vitro and in patients with TDT. The subjects consumed a placebo or GTE tablets (50 mg and 2 × 50 mg EGCG equivalent) every day for two months. Blood was then collected from the treated patients for analyses of PLT numbers, agonist-induced PLT aggregation, and anti-coagulation proteins. In our findings indicate that the in vitro treatment of GTE (at least 1 mg EGCG equivalent) inhibited PLT aggregation in patients who were healthy and with thalassemia platelet-rich plasma (PRP), which was significant in the healthy PRP. Consistently, GTE treatment inhibited the PLT aggregation that had been ex vivo generated by collagen or ADP. In addition, consumption of GTE tablets greatly inhibited PLT aggregation and increased the plasma levels of proteins C and S, as well as the free protein S concentrations depending upon the time course, but not the GTE dosage. Moreover, plasma ferritin levels decreased in both green tea tablet groups in a time-dependent manner (p < 0.05 in the second month). In conclusion, EGCG-rich GTE diminished PLT aggregation in patients who were healthy and patients with thalassemia plasma. It also improved PLT aggregation and hypercoagulability in patients with TDT by increasing the antithrombotic activity of protein C and protein S. This would suggest an adjuvant of GTE could reduce the risk of thrombosis associated with iron overload.
Wheat Embryo Albumin and Its Peptide Alleviate Acute Exercise Fatigue as Energy Supplement
Authors: Liao Aimei , Li Xiaoxiao , Wang Yanbing , Ding Zhirui , Pan Long , Hou Yinchen , Liu Quanping , Li Jianzheng , Shang Menghui , Huang Jihong
Published: November 2024

Wheat embryo albumin (WEA), rich in amino acids with a good balanced proportion, demonstrates plentiful biological activities. The effects of WEA and its peptide with the best antioxidant ability (F3) as a post-workout and pre-workout energy supplement on alleviating acute exercise fatigue were investigated. Under two experimental cases, the exhaustion-to-death swimming time and exhaustion swimming time were determined. Fatigue-related biochemical indexes including lactate dehydrogenase (LDH), the level of blood urea nitrogen (BUN), alanine transaminase (ALT), aspartate transaminase (AST), liver glycogen (LG), and muscle glycogen (MG) were measured with commercial kits. Antioxidant capacity in vivo was analyzed by determining the content of malondialdehyde (MDA), the level of glutathione (GSH), and the activity of superoxide dismutase (SOD) based on colorimetric methods. The results indicated that administration of WEA and F3 post-workout or pre-workout significantly prolonged exhaustive swimming time (p < 0.05) and increased the levels of glycogen in the liver and muscle of mice (p < 0.05). Meanwhile, WEA and F3 significantly reduced the activities of ALT, AST, and LDH and the level of BUN compared with the ones of mice in an exercise fatigue model (p < 0.05). Additionally, in comparison with the model group, supplements of WEA and F3 obviously decreased the content of MDA while enhancing the activity of SOD and the level of GSH both in the liver and muscle of mice. These results demonstrated that WEA and F3 can mitigate exercise fatigue and are conducive to recovery from fatigue in exhausted mice. It suggests that WEA and its peptide F3 could be a promising energy supplementary material against fatigue caused by continuous or high-intensity exercise.
ADI Compact Difference Scheme for the Two-Dimensional Integro-Differential Equation with Two Fractional Riemann–Liouville Integral Kernels
Authors: Chen Ziyi , Zhang Haixiang , Chen Hu
Published: November 2024

In this paper, a numerical method of a two-dimensional (2D) integro-differential equation with two fractional Riemann–Liouville (R-L) integral kernels is investigated. The compact difference method is employed in the spatial direction. The integral terms are approximated by a second-order convolution quadrature formula. The alternating direction implicit (ADI) compact difference scheme reduces the CPU time for two-dimensional problems. Simultaneously, the stability and convergence of the proposed ADI compact difference scheme are demonstrated. Finally, two numerical examples are provided to verify the established ADI compact difference scheme.
Variable Time Step Algorithm for Transient Response Analysis for Control and Optimization
Authors: Reznichenko Igor , Podržaj Primož , Peperko Aljoša
Published: November 2024

This work considers an optimization problem based on step response characteristics. We lay a foundation for it by designing a rapid transient response analysis algorithm with variable time steps. This method applies to linear ordinary differential equations with real order. Numerical tests of the algorithm in the integer case show significant improvement even for higher order systems. This suggests a new method for acquiring step response characteristics for the fractional order case for which we have constructed an explicit expression of the inverse Laplace transform.
Assessing Shallow Groundwater Quality Around the Sheba Leather Tannery Area, Wikro, North Ethiopia: A Geophysical and Hydrochemical Study
Authors: Abera Kaleab Adhena , Asfaw Berhane Abrha , Doyoro Yonatan Garkebo , Gebreyohanes Tesfamichael , Hussien Abdelwassie , Berhane Gebremedhin , Hagos Miruts , Romha Abadi , Walraevens Kristine
Published: November 2024

This study aimed to investigate the shallow groundwater status around the Sheba Leather Tannery area, Wikro, North Ethiopia, through geophysical and hydrochemical methods. Seventeen Vertical Electrical Soundings (VESs) acquisitions, 4 upstream and 13 downstream, of the leather tannery area were conducted. Using the data, four geoelectric profiles were generated. The aquifers’ geoelectrical layers, depth, and lateral extent were delineated. The VES curves depicted three to four resistivity layers. These alternating layers of low, moderate, and high resistivity values, traced at different VES points, were attributed to the formations’ composition and the groundwater quality status. Besides the geophysical survey, 32 water samples were collected from the area. Parameters such as electrical conductivity (EC), total dissolved solids (TDSs), pH, major ions, and heavy metals were analyzed. Moreover, PHREEQC was used to determine the groundwater mineral saturation indices where most minerals, except halite, were found supersaturated. The quality status for drinking purposes was also evaluated using the water quality index (WQI), and the water was classified as good (56.3%), poor (37.5%), and very poor (6.2%). The sodium adsorption ratio (SAR) and the percentage of sodium (Na+%) were calculated, and the results indicated that the water is suitable for direct use in irrigation.
Characterizing Managerial Decision Making in Public Hospitals: A Case Study from Romania
Authors: Cumpăt Carmen Marinela , Huțu Daniela , Rusu Bogdan , Zouri Muthana , Zouri Nicoleta
Published: November 2024

Background/Objectives: Our study investigates the primary characteristics of managerial decision-making processes in the public hospital units in Romania, particularly in the Northeast region. This research aims to delineate the decision-making model applied by managers in these units, considering the multitude of legislative, economic, technical, ethical, and organizational changes prompted by the pandemic. Methods: A mixed-method research approach was utilized, combining semi-structured interviews and autoethnography, to capture experiences, attitudes, perceptions, motivations, and ethical considerations of decision-makers within the healthcare system. Results: The findings revealed that managerial decisions in public hospitals were influenced by unique elements such as the vulnerability and support needs of patients, the absence of a clear hierarchy, the personalized nature of healthcare services, the complexity of care processes, and the use of advanced technology. External factors, notably political and economic influences, alongside internal ethical dilemmas, significantly impacted decision making. Conclusions: This study identifies the reliance on evidence-based decision making and a consultative managerial style as key to addressing these challenges. This research contributes theoretically by comparing decision-making models and practically by identifying a decision-making model that includes forms, techniques, and tools that could guide managers in decision making in Romanian public hospitals.
Anonymity in Dealer-to-Customer Markets
Authors: Di Cagno Daniela T. , Paiardini Paola , Sciubba Emanuela
Published: November 2024

We use a laboratory experiment to explore the effect of a change in pre-trade anonymity in a quote-driven dealer-to-customer market, organised as a request for quote (RFQ). We consider two treatments in which dealers interact with two types of customers (informed or uninformed). In the first treatment, there is no anonymity: dealers know whether the customer that sent them the request for quote is informed or uninformed. In the second treatment, there is complete anonymity: dealers do not know the type of customers they are interacting with. We find that anonymity improves price efficiency, whereas it does not adversely impact dealers’ trading profits. Our results contribute to the debate on transparency versus the adoption of anonymity in financial markets.
Antibiofilm Activities of Multiple Halogenated Pyrimidines Against Staphylococcus aureus
Authors: Sim MinHwi , Kim Yong-Guy , Lee Jin-Hyung , Lee Jintae
Published: November 2024

Staphylococcus aureus, prevalent in hospital and community settings, forms biofilms that are highly resistant to antibiotics and immune responses, complicating treatment and contributing to chronic infections. These challenges underscore the need for novel treatments that target biofilm formation and effectively reduce bacterial virulence. This study investigates the antibiofilm and antimicrobial efficacy of novel halogenated pyrimidine derivatives against S. aureus, focusing on three compounds identified as potent biofilm inhibitors: 2,4-dichloro-5-fluoropyrimidine (24DC5FP), 5-bromo-2,4-dichloro-7H-pyrrolo[2,3-d]pyrimidine (24DC5BPP), and 2,4-dichloro-5-iodo-7H-pyrrolo[2,3-d]pyrimidine (24DC5IPP). The three active compounds are bacteriostatic. In particular, 24DC5FP at 5 µg/mL achieved a 95% reduction in hemolysis with a minimum inhibitory concentration (MIC) of 50 µg/mL. Interestingly, 24DC5FP increased cell size and produced wrinkled colonies. qRT-PCR analysis showed that 24DC5FP suppressed the gene expressions of agrA and RNAIII (quorum sensing regulator and effector), hla (α-hemolysin), nuc1 (nucleases nuc1), and saeR (S. aureus virulence regulator). These findings suggest that extensive halogenation enhances the antibiofilm and antivirulence activities of pyrimidine derivatives, offering a promising strategy for combatting S. aureus infections, including those resistant to conventional treatments.
Gut Microbiota–microRNA Interactions and Obesity Pathophysiology: A Systematic Review of Integrated Studies
Authors: Azari Hushyar , George Megan , Albracht-Schulte Kembra
Published: November 2024

Obesity is the fifth leading cause of death globally and its comorbidities put a high burden on societies and cause disability. In this review, we aim to summarize the interactions and crosstalk between gut microbiota and micro-RNA (miRNA) in obesity. We searched for the relevant literature through PubMed, Web of Science, Scopus, and Science Direct. The study design is registered in the international prospective register of systematic reviews (Prospero). According to the inclusion criteria, eight studies were eligible for assessment (two studies including human subjects and six studies including animal subjects). We report that the interactions of miRNA and gut microbiota in the context of obesity are diverse and in some cases tissue specific. However, the interactions mediate obesity-associated pathways including the inflammatory response, oxidative stress, insulin signaling, gut permeability, and lipogenesis. To mention the most meaningful results, the expression of adipose tissue miRNA-378a-3p/5p was associated with Bifidobacterium and Akkermansia abundance, the expression of hepatic miRNA-34a was related to the Firmicutes phylum, and the expression of miRNA-122-5p and miRNA-375 was associated with the Bacteroides genus. miRNA-microbiota-associated pathological pathways seem to provide an intricate, but promising field for future research directed toward the treatment of obesity and its comorbidities.
Notch Inhibitors and BH3 Mimetics in T-Cell Acute Lymphoblastic Leukemia
Authors: Sergio Ilaria , Varricchio Claudia , Squillante Federica , Cantale Aeo Noemi Martina , Campese Antonio Francesco , Felli Maria Pia
Published: November 2024

T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy with poor response to conventional therapy, derived from hematopoietic progenitors committed to T-cell lineage. Relapsed/Refractory patients account for nearly 20% of childhood and 45% of adult cases. Aberrant Notch signaling plays a critical role in T-ALL pathogenesis and therapy resistance. Notch inhibition is a promising therapeutic target for personalized medicine, and a variety of strategies to prevent Notch activation, including γ-secretase (GS) inhibitors (GSIs) and antibodies neutralizing Notch receptors or ligands, have been developed. Disruption of apoptosis is pivotal in cancer development and progression. Different reports evidenced the interplay between Notch and the anti-apoptotic Bcl-2 family proteins in T-ALL. Although based on early research data, this review discusses recent advances in directly targeting Notch receptors and the use of validated BH3 mimetics for the treatment of T-ALL and their combined action in light of current evidence of their use.
Transcriptome and Temporal Transcriptome Analyses in Single Cells
Authors: Lyu Jun , Chen Chongyi
Published: November 2024

Transcriptome analysis in single cells, enabled by single-cell RNA sequencing, has become a prevalent approach in biomedical research, ranging from investigations of gene regulation to the characterization of tissue organization. Over the past decade, advances in single-cell RNA sequencing technology, including its underlying chemistry, have significantly enhanced its performance, marking notable improvements in methodology. A recent development in the field, which integrates RNA metabolic labeling with single-cell RNA sequencing, has enabled the profiling of temporal transcriptomes in individual cells, offering new insights into dynamic biological processes involving RNA kinetics and cell fate determination. In this review, we explore the chemical principles and design improvements that have enhanced single-molecule capture efficiency, improved RNA quantification accuracy, and increased cellular throughput in single-cell transcriptome analysis. We also illustrate the concept of RNA metabolic labeling for detecting newly synthesized transcripts and summarize recent advancements that enable single-cell temporal transcriptome analysis. Additionally, we examine data analysis strategies for the precise quantification of newly synthesized transcripts and highlight key applications of transcriptome and temporal transcriptome analyses in single cells.
Medication Exposure and Risk of Dementia and Alzheimer’s Disease
Authors: Sharma Niti , An Seong Soo A. , Kim Sang Yun
Published: November 2024

Alzheimer’s disease (AD), a complex neurodegenerative disease (ND), is the most predominant cause of dementia among the elderly. Generally, elderly people have multiple chronic health conditions, like hypertension, arthritis, diabetes, insomnia, bowel problems, and depression. Although prescribed medications have beneficial therapeutic compositions, some may have side effects that could hinder cognitive function or worsen cognitive decline. Hence, we should evaluate those medications to guarantee their safety. In the present mechanistic review, we discussed frequently used categories of medication (analgesics, anticholinergics, benzodiazepines, proton pump inhibitors, and statins), concerning their possible involvement in increasing AD and dementia risks. This review summarized the results of various observational studies, meta-analyses, randomized case–control studies, and systematic reviews. As the results were contradictory, it was difficult to ascertain the clear associations between medication usage and increased risks of dementia or AD. The blood-based biomarkers (BBMs) offer a low-cost and accessible alternative for early diagnosis of AD. Systematic reviews combined with meta-analysis would be crucial tools for accurately assessing and summarizing the efficacy of health interventions, yet randomized clinical trials have always been the best way to help with clinical care decisions. Thus, an open discussion is necessary to help individuals determine whether the advantages of utilizing medications outweigh the possible drawbacks.
Maternal Low-Protein Diet Leads to Mitochondrial Dysfunction and Impaired Energy Metabolism in the Skeletal Muscle of Male Rats
Authors: Vidyadharan Vipin A. , Betancourt Ancizar , Smith Craig , Blesson Chellakkan S. , Yallampalli Chandra
Published: November 2024

A prenatal low-protein (LP) diet disrupts glucose homeostasis in adult offspring. Skeletal muscles are one of the main sites of glucose clearance, and mitochondria residing in the muscle fibers are central to glucose homeostasis. Our previous studies indicated that impaired mitochondrial health is central to dysregulated glucose metabolism in the gastrocnemius muscle of the LP-programmed female rats. In addition, dysfunctional mitochondria are often an indicator of underlying irregularities in energy metabolism and metabolic inflexibility. Therefore, this study examined the mitochondrial function and metabolic flexibility in the skeletal muscles of prenatal LP-programmed adult male rats. Pregnant Wistar rats were randomly allotted to a control diet (20% protein) or an isocaloric LP diet (6% protein). Standard laboratory rat chow was given to the dams and the pups after delivery and weaning. Gene and protein expressions, mtDNA copy number, and electron microscopy were assessed in gastrocnemius (GS) muscle, and the mitochondrial oxygen consumption rate was determined using isolated flexor digitorum brevis muscle fibers. The genes associated with mitochondrial outer membrane fusion, mitofusin1 and 2 (Mfn1 and Mfn2), fission (Fis1), and biogenesis (Pgc1B, Nrf1, and Esrra) were lower in the LP group. Further, our functional studies showed that the ATP-linked oxygen consumption rate (OCR), maximal, spare respiratory, and non-mitochondrial respiration-associated OCRs were lower in the LP rats. Further, the mRNA and protein expressions of Ndufb8, a key factor involved in the complex-I catalytic activity, were downregulated in the LP group. In addition, the expression of genes linked to mitochondrial pyruvate transport (Mpc1) and metabolism (Pdha1) was lower in the LP group. In contrast, the expression of mitochondrial fatty acid transporters (Cpt1a and Cpt2) was higher in the LP when compared to the control group. However, electron microscopic analysis exhibited no difference in the mitochondrial ultrastructure in the LP muscle compared to the control. Altogether, our results indicate that the LP diet affects the mitochondrial complex-I integrity and dynamics and leads to altered expression of genes associated with substrate oxidation and mitochondrial dysfunction in the skeletal muscle of the male LP offspring.
Early Learning Curve in Robotic-Assisted Total Knee Arthroplasty: A Single-Center Experience
Authors: Putzer David , Schroeder Lennart , Wassilew Georgi , Liebensteiner Michael , Nogler Michael , Thaler Martin
Published: November 2024

Background/Objectives: This study evaluated the learning curve for robotic-assisted total knee arthroplasty (RA TKA) performed by three experienced surgeons, focusing on procedure duration, surgeon satisfaction, and confidence. Methods: A prospective study was conducted with three senior arthroplasty surgeons, each performing 15 RA TKA procedures using the Triathlon Knee System with the Robotic Arm Interactive Orthopedic (RIO) System. Data on preparation, cut-to-suture, and breakdown times were collected. Surgeon anxiety levels were measured preoperatively using the STAI-6 scale, while postoperative satisfaction and confidence were assessed via a questionnaire. Statistical analysis was conducted using GraphPad Prism. Results: Of 50 scheduled surgeries, 45 were completed. The average cut-to-suture time was 1 h 38 min, with significant time reductions in robotic-specific steps as experience increased. Comparing the first five surgeries to the last five, the time for navigation hardware mounting, landmarks registration, femur and tibia registration, and bone preparation decreased by up to 30% (p < 0.001 to p = 0.025). General instrument preparation time decreased by 20% (p = 0.004). Surgeon anxiety levels dropped, indicating increased comfort with the system, while postoperative surveys showed increased satisfaction and confidence. Conclusions: The study demonstrated a substantial learning curve for RA TKA, with improved efficiency and surgeon confidence by the fifteenth procedure. These findings highlight the potential for streamlined workflows and guide training for new adopters of robotic knee arthroplasty.
Comparison of the Effects of Ultrasound-Guided Subacromial Injection Versus Subacromial Injection and Suprascapular Nerve Block on Pain, Function, and Sleep Quality in Rotator Cuff Lesions
Authors: Haciomeroglu Mustafa , Takmaz Suna Akin , Serce Azize , Karaduman Yilmaz , Basar Hulya
Published: November 2024

Background/Objectives: Rotator cuff lesions are common causes of shoulder pain. When not treated effectively, the functional loss associated with pain affects the quality of life and brings about psychosocial issues. In this study, prospective observational comparison of the effects of ultrasonography (USG) guided subacromial injection (SAI) versus subacromial injection combined with suprascapular nerve block (SSNB) on pain, functionality and sleep quality in the treatment of shoulder pain unresponsive to conservative treatments due to rotator cuff lesions is made. Methods: The data of 25 patients in both groups were compared prospectively. Patients were evaluated after 30 min, 1 week, 2 weeks, 1 month, and 3 months. Pain levels were measured with VAS, shoulder functions with SPADI and sleep quality with PSQI. Analgesic consumption and satisfaction were also recorded. Results: Both treatment groups effectively reduced pain at rest over the 3-month follow-up period. However, the SAI group did not achieve the targeted level of analgesia for pain control during movement. In comparison, the SAI + SSNB group demonstrated significantly superior outcomes, with lower VAS scores both at rest and during motion, as well as improved SPADI and PSQI scores. Additionally, analgesic consumption was significantly reduced in the SAI + SSNB group. No side effects or complications were observed during the treatment applications or the follow-up period. Conclusions: Pain control, shoulder functionality, sleep quality, and patient satisfaction were found to be higher in patients treated with SAI + SSNB in the short-to-medium term in the treatment of shoulder pain due to rotator cuff lesions, in addition to lower analgesic consumption.
Contribution of Shockwave Therapy in the Functional Rehabilitation Program of Patients with Patellofemoral Pain Syndrome
Authors: Neculăeș Marius , Hernandez-Lucas Pablo , Ioana-Bianca Ioja , Lucaci Paul
Published: November 2024

Background: Patellofemoral pain syndrome is a condition with an increasing incidence in recent years, being known as the most common cause of knee pain in adults and adolescents. Undiagnosed and untreated, this condition can worsen over time. The aggravation leads to an increase in the intensity of the pain and the risk of injury, along with an increase in stress on the other joints of the lower limb. The objective of this study was to evaluate the contribution of shockwave therapy to a functional rehabilitation programme for patients with patellofemoral pain syndrome. Materials and Methods: The study was carried out on a group of 64 subjects (32 males and 32 females), aged between 20 and 39 years. The subjects were divided into two groups: 32 subjects who followed a program of functional rehabilitation based on low- and medium-frequency electrotherapy, ultrasound and laser therapy, along with a physical therapy program lasting approximately 3 weeks, and 32 subjects who followed a functional rehabilitation program based on shockwave therapy and specific physical therapy exercises lasting approximately 3 weeks. Results: Following the protocols applied to the two groups, the pain reported by the patients decreased, while the functional parameters of the knee improved, better results being obtained in the group that performed shock wave therapy together with specific physical therapy programs (Cohen Index 5916, p < 0.001). Conclusions: This study indicates that radial shockwave therapy combined with physiotherapy may provide additional benefits for patellofemoral syndrome, including greater pain reduction and improved joint mobility, compared to traditional treatments. However, further research is needed to confirm these findings and their broader clinical applicability.
Treatment Sequencing and Independent Outcomes of First- and Second-Line Chemotherapy in a Retrospective Series of Patients with Biliary Tract Cancer
Authors: Frega Giorgio , Palloni Andrea , Deiana Chiara , Rizzo Alessandro , Ricci Angela Dalia , Brandi Giovanni
Published: November 2024

Background/Objectives: Biliary tract cancers (BTCs) are aggressive neoplasms with limited therapeutic options. The amount of prospective evidence is poor, and limited data are available on the impact of treatment sequencing on survival. Here we report a real-world experience of patients with advanced BTC treated with at least three lines of therapy. We evaluated the impact of sequential treatments, and we further compared the efficacy of Gemcitabine/Cisplatin (GemCis) and mFOLFOX to other first- and second-line chemotherapy regimens, respectively. Methods: Data on 60 patients with locally advanced or metastatic BTC under the care of a single Italian referral hospital and treated with at least three lines of chemotherapy were retrospectively collected. Data from 56 patients were included in the analysis. Survival analyses were performed using R software (v1.2.5042). Results: We compared the outcomes of patients treated according to the “standard” pre-immunotherapy sequence (GemCis and mFOLFOX in the first and second lines, respectively) versus those treated with all other combinations (“control” group). Our analysis did not show significant survival differences between the two groups. However, it should be noted that we selected long-survival patients by including only those who received at least three or more lines of chemotherapy. Focusing on the first-line setting, no significant differences in both mPFS and mOS emerged by comparing GemCis versus other doublets (mainly Gemcitabine/Oxaliplatin). Similarly, mPFS and mOS from second-line treatment did not statistically differ between patients treated with mFOLFOX versus those treated with other regimens (71% chemotherapy doublets). Conclusions: Our series provides real-world outcomes of patients with advanced BTC before the approval of immunotherapy. Even considering the monocentric and retrospective design, our study represents one of the first analyses on the impact of sequential treatment strategies in patients with BTC.
Evaluation of Triglyceride Glucose Index in Patients with Patent Foramen Ovale Who Experienced Cryptogenic Stroke
Authors: Ayça Burak , Yıldız Cennet , Yüksel Yasin , Katkat Fahrettin , Arpaç Atakan , Çağlar Fatma Nihan Turhan , Erkol Cansu
Published: November 2024

Background/Objectives: The prevalence of patent foramen ovale (PFO) has been found to be increased in patients with cryptogenic stroke, suggesting an association between these two clinical settings. Insulin resistance is a risk factor for the occurrence of stroke. The triglyceride glucose (TyG) index is a biomarker that reflects the IR status of the body. Our aim was to evaluate the TyG index values in patients with PFO who experienced cryptogenic stroke. Methods: One hundred and twenty nine patients with PFO who experienced embolic stroke and one hundred and eight control subjects were enrolled. All patients in the study group experienced embolic stroke within 2 weeks of enrollment. The TyG index value of each patient was calculated. Results: Patients with stroke were significantly older, had higher levels of glucose, creatinine, triglyceride (TG), leukocyte, and TyG index and lower high-density lipoprotein–cholesterol values. The TyG index had the highest sensitivity for the prediction of stroke in comparison to TG and glucose values. Comparison of ROC curves showed that the TyG index had the highest AUC compared to that of TG and glucose. The TyG index value of 8.89 predicted stroke occurrence with a sensitivity and specificity of 63.2% and 72.3%, respectively. The results of multivariable regression analyses showed that the TyG index had a higher odds ratio than TG, which indicated that it had a better predictive value. Conclusions: Assessment of the TyG index in cryptogenic stroke patients with PFO might be helpful for the management of these patients.
Preparation and Characterization of a Novel Hydroxyapatite-Capsaicin Composite Intended for the In-Office Dental Bleaching Use
Authors: da Silva Karine Letícia , Hortkoff Diego , Favoreto Michael Willian , Rezende Marcia , Nadal Jessica Mendes , Armas-Vega Ana , Loguercio Alessandro D. , Farago Paulo Vitor
Published: November 2024

The objective of this research was to synthetize a hydroxyapatite-capsaicin composite (HAp-CAP) and assess its physicochemical properties for potential use in an in-office dental bleaching gel. The HAp-CAP nanocomposite was prepared by the precipitation method using a 4:1 ratio of nano-hydroxyapatite (nano-HAp) to capsaicin (CAP) dissolved in 99.5% ethyl alcohol (q.s.p) with the assistance of zirconia beads in a SpeedMixer®. Characterization of HAp-CAP involved morphological, spectroscopic, and thermal analyses. Subsequently, HAp-CAP was incorporated into an in-office dental bleaching agent to evaluate its impact on reducing hydrogen peroxide diffusion into the pulp chamber and its effect on tooth color alteration during the bleaching process. The HAp-CAP in a bleaching gel slightly decreased the amount of hydrogen peroxide inside the pulp chamber and did not interfere in the bleaching effectiveness of dental bleaching. The physicochemical characterization indicates that the synthesis process of the HAp-CAP composite was successfully carried out, and its use in dental materials poses a challenging task that warrants further investigation since its incorporation.
Advanced Modelling and Performance Analysis of a Separately Excited Direct-Current Motor Powered by Photovoltaic Generators Using Maximum Power Point Tracking Techniques
Authors: Alasali Feras , Sweidan Tha’er O. , Abuashour Mohammed I. , Holderbaum William
Published: November 2024

The integration of photovoltaic (PV) systems into DC motor drives has prompted the enhancement of motor performance. This study explores the application of photovoltaic generators (PVs) to independently power and excite a Separately Excited Direct-Current (SEDC) system by utilizing a proportional open-circuit voltage method as a strategy for tracking the maximum power point. This approach offers an effective means of optimizing energy output from PV systems. The primary aim was to optimize power output from photovoltaic generators across varying solar intensity levels. This paper describes the nonlinear current/voltage behaviour of PV generators under different levels of irradiation, along with the magnetic characteristics of the core material in an SEDC motor, utilizing advanced polynomial equations for accurate mathematical representation. Furthermore, we conducted a dynamic analysis of the SEDC motor, powered by the PV generators, under varying solar intensities. This study investigates the operational performance of the SEDC motor under varying solar irradiance levels by developing a realistic model using MATLAB software, R2022a, for numerical simulations, followed by implementation on high-performance computing platforms, including a real-time simulator and testbed, using a real-time digital simulator (RTDS).
The Association of Benthic Infauna with Fine-Grained Organic-Rich Sediments in a Shallow Subtropical Estuary
Authors: Hope Daniel , Cox Anthony , Zamora-Duran Angelica , Johnson Kevin B.
Published: November 2024

Fine-grained organic-rich sediments (FGORSs) from anthropogenic impacts are a growing concern for bays and estuaries around the world. This study explores the relationships of infaunal community diversity and species abundances with FGORSs in the Indian River Lagoon and its tributaries. To examine these potential relationships, infauna was collected monthly using a Petite Ponar grab at 16 stations in the central Indian River Lagoon from October 2015 to August 2016. Abundant taxa in these sediments include polychaete worms (e.g., the polychaete Nereis succinea), mollusks (e.g., clam Parastarte triquetra), and arthropods (e.g., the tanaid Leptochelia dubia), with densities as high as 5.3 × 104 m−2 (L. dubia in July 2016). Increasing organic matter (OM) in the sediments was inversely correlated with species richness (R2 = 0.75; p-value < 0.001), densities (R2 = 0.69; p-value < 0.001), and diversity (R2 = 0.37; p-value < 0.001). Other infaunal community and population data showed similar relationships with silt–clay (%), sediment porosity, and dissolved oxygen (mg L−1). Two thresholds of OM and correlated environmental parameters are discussed: an impairment threshold at 2% OM, above which infauna decreases precipitously, and a critical threshold at 10% OM, above which infauna is generally absent.
The Path Tracking Control of Unmanned Surface Vehicles Based on an Improved Non-Dominated Sorting Genetic Algorithm II-Based Multi-Objective Nonlinear Model Predictive Control Method
Authors: Guo Yunzhe , Zhu Qidan , Mou Jinyou
Published: November 2024

This paper proposes a multi-objective nonlinear model predictive control (MOMPC) method based on an improved non-dominated sorting genetic algorithm II (NSGAII) for the path tracking problem of unmanned surface vehicles (USVs). To enhance performance in cross-track error, a varying look-ahead distance is utilized in the line of sight (LOS) algorithm, which allows the MPC control algorithm to compute the look-ahead distance and desired speed rather than directly calculating the control input. Since the cost function of the MPC algorithm includes multiple objective terms, a multi-objective model predictive control algorithm is employed to improve overall control performance. Additionally, an adaptive rotation-based simulated binary crossover (ARSBX) is integrated into the NSGAII algorithm, and the non-dominated sorting method is optimized to reduce computation time. These enhancements increase diversity and exploration in the solution space, enabling the algorithm to find the optimal solution more efficiently. Simulations conducted in two different scenarios demonstrate that the nonlinear MPC method based on the improved NSGAII successfully tracks the desired path; it achieved an improvement of approximately 41% in time performance and about 5% in path-tracking error performance, exhibiting strong control performance and robustness.
The Impact of CEO Characteristics on Investment Efficiency in Jordan: The Moderating Role of Political Connections
Authors: Shaheen Loona , Alatyat Zakarya , Aldabbas Qasem , Abu Shihab Ruba Nimer , Abuaddous Murad
Published: November 2024

This study investigates the impact of CEO characteristics—specifically CEO age, founder status, and family membership—on investment efficiency in Jordanian non-financial companies, with a focus on the moderating role of political connections. Drawing on the existing literature, we identify conflicting views regarding how these characteristics influence investment decisions. Some studies suggest that younger CEOs may adopt more aggressive investment strategies, while older CEOs tend to be conservative, leading to balanced resource allocation. Similarly, CEOs with founder status and family membership are thought to have an emotional attachment to the company, theoretically resulting in cautious investment behavior. However, empirical evidence remains mixed. By using data from 62 non-financial firms listed on the Amman Stock Exchange (ASE) from 2019 to 2023, this study employs regression analysis to explore these relationships. The findings reveal that CEO age contributes to investment efficiency by mitigating both over- and under-investment. Contrary to expectations, CEO founder status shows no significant effect on investment efficiency. Additionally, family-member CEOs exhibit a tendency toward under-investment, driven by a desire to preserve family wealth. Political connections further complicate these dynamics, encouraging riskier investment strategies while diluting the positive effects of CEO characteristics. These results provide new insights into the intricate interplay between CEO traits and political networks, contributing to the discourse on corporate governance in emerging markets. The study concludes with practical implications for policymakers and company boards, emphasizing the need for balanced leadership selection strategies to optimize investment efficiency.
Evaluation of a Great Agrovoltaic Implementation in an Isle Using SWOT and TOWS Matrices: Case Study of Gran Canaria Island (Spain)
Authors: Pulido-Alonso Antonio , Quintana-Suárez José C. , Rosales-Asensio Enrique , Feo-García José J. , Florido-Suárez Néstor R.
Published: November 2024

Nowadays, we are heading towards global decarbonisation, with each sector involved contributing partial solutions to the problem, without realising that an overall vision is necessary. Photovoltaics emerged as a technology that requires a lot of surface area, which is why it has been integrated into buildings and other human infrastructures (BPVI). The effects of the implementation of AVS on an island have been analysed, observing the territory’s energy use, population, and social and topographical realities, collecting all the peculiarities that could be affected by a massive implementation of this technology. The method to be followed is a SWOT and TOWS analysis, widely employed in all types of scientific studies. The increase in the island’s resilience has been assessed, as has its decreasing its dependence on the outside. In this case, it has been observed that conventional PV is currently being installed on agricultural land to decarbonise electricity production, which mostly relies on oil and does not consider that the island is a territory with a high food dependence on the outside; a high unemployment rate; a high factor of soil desertification, meaning fires are frequent; a high rate of abandonment of agricultural land; and a shortage of flat land. Therefore, we affirm that the island’s carbon footprint will increase by not taking all these factors into account. In addition to punishing the local economy by destroying fertile soil, local food and jobs, the current method of energy production increases the need for subsidies to import food products from abroad. In addition, we claim that the use of AVS reduces the water needs of the crop, which is relevant on an island with great water scarcity. It is concluded that 11 of the 17 UN Sustainable Development Goals would be improved with the use of agrovoltaic technology.
Approaches to Urban Landscape Transformation vs. Heritage
Authors: Bojanić Obad Šćitaroci Bojana , Omićević Nerma , Zaninović Tamara
Published: November 2024

Cities are systems composed of urban and natural landscapes with intangible and tangible layers, continuously developing and overlapping [...]