bluuebunny/crossref_metadata_2025 huggingface dataset:
CD147 promotes melanoma cell growth via SOX4-mediated glycolytic metabolism
Authors: Sun Xiaohui , Yang Pengfei , Jiang Yuan
Published: March 2021

Purpose: To determine the functional roles of cluster of differentiation 147 (CD147) in glycolysis in melanoma cells.Methods: CD147 expression in melanoma tissue and adjacent normal tissue was determined using quantitative real time polymrase chain reaction (qRT-PCR) and immunohistochemistry. Cell Counting Kit-8 (CCK-8) and colony formation assays were used to evaluate cell viability and colony formation, respectively. The role of CD147 in glycolysis in melanoma cells was investigated by determining glucose uptake, production of lactate, and cellular level of ATP.Results: CD147 was enhanced more in melanoma tissue than that in the adjacent normal tissue (p < 0.001). CD147 overexpression promoted the viability and colony formation of melanoma cells. On the other hand, CD147 silencing decreased the viability and colony formation of melanoma cells. Glucose uptake, production of lactate, and cellular level of ATP were upregulated in melanoma cells by CD147 overexpression and downregulated by shRNA-mediated depletion of CD147. CD147 increased expression of C-X-C motif chemokine ligand 1 (CXCL1) to activate the sex-determining region Y-related high-mobility group box 4 (SOX4) pathway. Knockdown of CXCL1 attenuated the positive regulatory effect of CD147 on SOX4. Besides, overexpression of SOX4 reversed the suppressive effects of CD147 silencing on melanoma cell viability, colony formation, and glycolysis.Conclusion: CD147 contributes to melanoma cell growth via upregulation of SOX-mediated glycolysis, thus providing a therapeutic avenue for the management of melanoma.

Keywords: Cluster of differentiation 147, CD147, Sex-determining region Y-related high-mobility group box 4, Melanoma, Cell growth, Glycolysis
Upregulation of miR-195a inhibits brain-derived neurotrophic factor in mouse hippocampus and may contribute to depression-like behaviors induced by chronic social stress
Authors: Wen Xuping , Lin Mingshuan
Published: March 2021

Purpose: To explore the effect of miR-195a on nerve cells in the hippocampal region of depressionmodel mice.Methods: A chronic social defeat stress (CSDS) model was used as a depressed mouse model. In vivo, C57BL/6J mice received CSDS treatment or miR-195a antagomir. Depression-like behaviors were evaluated. In vitro, the target relationship between miR-195a and brain-derived neurotrophic factor (BDNF) was validated by luciferase reporter assays in HEK-293 cells. In primary cortical neurons, expression levels of miR-195a and BDNF mRNA were evaluated using quantitative polymerase chain reaction (qPCR). BDNF protein expression was determined by western blotting.Results: The sucrose preference ratio and social contact of the CSDS group were significantly decreased, whereas the immobility time was significantly increased, compared with the control group (p< 0.05). Interestingly, the expression of miR-195a was upregulated in the CSDS group compared with control group (p < 0.05). Bioinformatics prediction and luciferase reporter assay data indicate that miR195a bound the BDNF 3’ untranslated region. BDNF protein expression levels were significantly reduced by miR-195a mimic but increased by miR-195a inhibitor, compared with the negative control mimic group (p < 0.05). In vivo, miR-195a antagomir alleviated depression-like behaviors compared with CSDS group. In addition, miR-195a antagomir restored the expression of BDNF in mouse hippocampus in the CSDS group (p < 0.05).Conclusion: MiR-195a inhibitor ameliorates depression-like behaviors of depressed mice by downregulation of BDNF, whereas  upregulation of miR-195a inhibits BDNF expression in mouse hippocampus and may contribute to depression.

Keywords: Chronic social defeat stress, Depression, MiR-195, brain-derived neurotrophic factor, BDNF
Betulinic acid inhibits glioma cell viability by downregulation of NF-κB and enhancement of apoptosis
Authors: Yaozu Zhu , Liu Yang , Zhao Huang , Peng Peng , Tingbao Zhang , Jincao Chen
Published: March 2021

Purpose: To determine the inhibitory potential of betulinic acid on pro-survival signaling pathway in glioblastoma.Methods: Changes in viabilities of glioma cells and primary astrocytes were measured using 3-(4, 5dimethylthiazol-2-yl)-2,  5-diphenyltetrazolium bromide (MTT) assay. Apoptotic changes were analyzed using Hoechst 33342 staining and Annexin V-FITC/PI kits. Western blotting was used for assaying the protein expressions of various pro-apoptotic and anti-apoptotic factors.Results: The proliferative potential of U87MG and A172 cells were significantly reduced on treatment with betulinic acid in a concentration- and time-dependent manner. Treatment with betulinic acid at a dose of 8.75 µg/mL increased apoptosis in U87MG and A172 cells to 41.8 ± 0.5 and 48.8 ± 0.5%, respectively (p < 0.05). Betulinic acid significantly decreased intracellular levels of NFκB p65 andsuppressed levels of survivin, XIAP and Bcl-2 in U87MG and A172 cells (p < 0.05). However, betulinic acid significantly increased the levels of Bax and activated caspase-9 and caspase-3 in U87MG and A172 cells (p < 0.05).Conclusion: Betulinic acid inhibited the proliferation of U87MG and A172 glioblastoma cells and mediated their apoptosis. There is need for in vivo studies for validation of the therapeutic potential of betulinic acid as an anti-glioblastoma drug.

Keywords: Glioblastoma, Betulinic acid, Proliferation, Apoptosis, Chemotherapy, Intracranial malignancy
Einstein doubly warped product manifolds with semi-symmetric metric connection
Authors: Gupta Punam , Diallo Abdoul Salam
Published: March 2021

In this paper, we study the doubly warped product manifolds with semi-symmetric metric connection. We derive the curvature formulas for doubly warped product manifold with semi-symmetric metric connection in terms of curvatures of components of doubly warped product manifolds. We also prove the necessary and sufficient condition for a doubly warped product manifold to be a warped product manifold. We obtain some results for an Einstein doubly warped product manifold and Einstein-like doubly warped product manifold of class A with respect to a semi-symmetric metric connection.
Existence of complex structures on decomposable Lie algebras
Authors: Sroka Marcin
Published: March 2021

We provide the classification of the six-dimensional decomposable Lie algebras, with the dimension of the biggest indecomposable summand less than five, admitting complex structures.
Data Journalism. Visualization of Data as a Remedy of Perception Problems of the Modern Reader
Authors: Dukalska-Hermut Joanna , Dziwak Ewelina
Published: March 2021

The consistent increase of information is not directly proportional to the increase in attention. Despite the availability of infinite amounts of content, the human brain, working on ‘old software’ cannot cope with the excess. As a result, anxiety and depression are deepening, which negatively affects the condition of modern man. Perceptual constraints are the reason for poverty of attention with the simultaneous richness of content. The aim of the conducted research is to demonstrate the superior role of image over text and to present data journalism and infographics as a remedy for the ill-conceived perceptual limitations of the modern ‘new media’ users. Scientific publications on data journalism, information visualization, infographics, databases, and human perception is the primary research material in this publication. The research method used for this work was the content analysis and literature studies. The conducted research allowed the authors to draw conclusions assuming that data journalism is conducive to visualization and may become a way of reliable information provision.

Data Journalism. Wizualizacja danych jako remedium na problemy percepcyjne współczesnego odbiorcy

Stale powiększająca się liczba informacji nie jest wprost proporcjonalna do wzrostu uwagi. Pomimo dostępności nieskończonych ilości treści ludzki mózg, pracując na „starym oprogramowaniu”, nie potrafi poradzić sobie z ich nadmiarem. W efekcie tego pogłębiają się stany lękowe i depresyjne, które negatywnie wpływają na kondycję współczesnego człowieka. Ograniczenia percepcyjne są powodem ubóstwa uwagi przy jednoczesnym bogactwie treści. Celem przeprowadzonych badań jest wykazanie nadrzędnej roli obrazu w stosunku do tekstu oraz przedstawienie dziennikarstwa danych i infografiki jako swoistego remedium na problemy percepcyjne współczesnego użytkownika nowych mediów. Materiałem badawczym stały się publikacje naukowe dotyczące dziennikarstwa danych, wizualizacji informacji, infografiki, baz danych i percepcji. Wykorzystana w pracy metoda badawcza to analiza zawartości i studia literaturowe. Przeprowadzone badania pozwoliły autorkom na wysnucie wniosku, że dziennikarstwo danych sprzyja wizualizacji i może stać się sposobem na rzetelne i niewywołujące lęku przekazywanie informacji.
On Scalarity in the Verbal Domain. The Case of Polish Psych Verbs. Part 1: Polish Perfective Psych Verbs and Their Prefixes
Authors: Willim Ewa
Published: March 2021

Polish perfective psych verbs are generally analyzed as inceptive predicates focusing the beginning of an emotional state holding of an experiencer. However, a perfective psych verb can also denote an event of gradual scalar change. In this paper, I argue that on the inceptive reading a perfective psych predicate denotes a transition from a state in which p does not hold to a state in which p holds of an experiencer. In events of gradual change, there is an increase in the degree on the scale of intensity of a given psych state or on the (abstract) extent scale contributed by a verb’s argument. As the internal temporal structure of the events denoted by perfective psych predicates can depend on elements of syntactic context outside the verb, the domain of aspectual composition in Polish is not the verb, pace Rothstein (2020), but VoiceP/vP.
Paradoks opłacalności. Stronniczość polityczna na polskim rynku medialnym – próba syntezy
Authors: Bartoszewicz Mateusz , Gołębiewski Filip
Published: March 2021

The study includes an attempt to synthesize the basic trends of political bias of 14 relevant media entities appearing on the Polish media market in a context of their readership and viewership. Searching for an intersubjectively consistent analytical perspective, the authors use critical and comparative analysis of the results of empirical research contained in the literature of the subject, as well as the content of specialized institutional reports, with a respect to the methods of critique of sources. Due to the processual nature of the development of the media market in Poland, some of the results of empirical research cited goes back to year 2010 but the vast majority concerns year 2015 and the next ones. In the introduction, the authors refer to the universal agreement of researchers of the quality of Polish public debate regarding its critical assessment. The first section of the article contains a description of the methodological assumptions made. The next three contain an attempt to synthesize the basic bias trends of relevant media titles within each of the three segments of media activity: [1] the press sector of nationwide dailies; [2] the weekly sector of opinion-forming magazines; and [3] the 24-hour news television sector. The last part of the study contains conclusions and reference to the research results of the political parallelism concept.
Wpływ pandemii COVID-19 na główne indeksy giełdowe na świecie
Authors: Jaworski Piotr
Published: March 2021

COVID-19 impact on the main global stock indexes The aim of the study is to examine the impact of information on lockdown connected with COVID-19 on stock indexes. The hypothesis was: it is a strong impact of the lockdown announcement on stock indexes at the time of its announcement or cancelation. Panel event models were used to verify the hypothesis. The study used data from the Thomson Reuters Database for the period from 2.01.2019–10.05.2020. The analysis was based on the papers and reports on COVID-19 and the literature on behavioral finance.
Body Composition and Metabolic Assessment After Motor Complete Spinal Cord Injury: Development of a Clinically Relevant Equation to Estimate Body Fat
Authors: Gater David R. , Farkas Gary J. , Dolbow David R. , Berg Arthur , Gorgey Ashraf S.
Published: March 2021

Background: Obesity is at epidemic proportions in the population with spinal cord injury (SCI), and adipose tissue (AT) is the mediator of the metabolic syndrome. Obesity, however, has been poorly appreciated in SCI because of the lack of sensitivity that body mass index (BMI) conveys for obesity risk in SCI without measuring AT. Objectives: The specific objectives were to compare measures of body composition assessment for body fat with the criterion standard 4-compartment (4C) model in persons with SCI, to develop a regression equation that can be utilized in the clinical setting to estimate fat mass (FM), and to determine cardiometabolic risk using surrogates of obesity in a current model of metabolic syndrome. Methods: Seventy-two individuals with chronic (>1 year) motor complete (AIS A and B) C5-L2 SCI were recruited over 3 years. Subjects underwent assessment with 4C using hydrostatic (underwater) weighing (UWW), dual-energy x-ray absorptiometry (DXA), and total body water (TBW) assessment to determine percent body fat (%BF); fasting glucose and lipid profiles, and resting blood pressure were also obtained. BMI, DXA, bioelectrical impedance analyses (BIA), BodPod, circumferences, diameters, lengths, and nine-site skinfold (SF) were assessed and validated against 4C. A multiple linear regression model was used to fit %BF (dependent variable) using anthropometric and demographic data that had the greatest correlations with variables, followed by a combined forward/backward stepwise regression with Akaike information criterion (AIC) to identify the variables most predictive of the 4C %BF. To allow for a more practical model for use in the clinical setting, we further reduced the AIC model with minimal loss of predictability. Surrogate markers of obesity were employed with metabolic biomarkers of metabolic syndrome to determine prevalence in persons with SCI. Results: Subject characteristics included age 44.4 ± 11.3 years, time since injury (TSI) 14.4 ± 11.0 years, BMI 27.3 ± 5.9 kg/m2; 59 were men and 13 were women. Sitting waist circumference (WCSit ) was 95.5 ± 13.1 cm, supine waist circumference (WCSup) was 93.4 ± 12.7 cm, and abdominal skinfold (ABDSF) was 53.1 ± 19.6 mm. Findings showed 4C %BF 42.4 ± 8.6%, UWW %BF 37.3 ± 9.7%, DXA %BF 39.1 ± 9.4%, BodPod %BF 33.7 ± 11.4%, nine-site SF %BF 37.8 ± 9.3%, and BIA %BF 27.6 ± 8.6%. A regression equation using age, sex, weight, and ABDSF provided R2 correlation of 0.57 with 4C %BF (p < .0001). Metabolic syndrome was identified in 59.4% of the sample. Conclusion: Body composition techniques to determine body fat are labor intensive and expensive for persons with SCI, and the regression equation developed against the criterion standard 4C model may allow clinicians to quickly estimate %BF and more accurately demonstrate obesity-induced cardiometabolic syndrome in this population.
Cuantificación de fenoles y flavonoides totales en un extracto blando de flores de Calendula officinalis Linn
Authors: Montané Ojeda Claudia , Arias Ramos Daily , Chil Núñez Idelsy
Published: March 2021

La Caléndula officinalis L. ocupa un lugar destacado en el tratamiento de múltiples afecciones, debido a sus propiedades farmacológicas, dentro de las que se incluyen antibacteriana, antiinflamatoria, cicatrizante, astringente, antiséptica, hipotensora, antiespasmódica, antibiótica, fungicida y antioxidante. Con el objetivo de cuantificar la proporción de compuestos fenólicos y flavonoides totales presentes en un extracto blando de flores de la planta, se elaboraron 5 lotes de extracto blando (EB) de Calendula officinalis L. La determinación cualitativa de fenoles totales y flavonoides reveló la presencia de estos compuestos en los extractos, lo que se confirmó con la determinación cuantitativa, revelando la presencia de un contenido de 9,65 a 11,31mgAG/0,1g y 1,51 a 2,17mgQ/0,1g de fenoles y flavonoides, respectivamente.
Pratique de l’agropastoralisme et changement climatique. Analyse des stratégies locales de résilience dans l’Extrême-Nord du Cameroun
Authors: MAMOUDOU Abdou-Raman
Published: March 2021

Dans les zones sahéliennes, l’agropastoralisme constitue la principale activité qui régit l’économie locale et occupe une part importante de la population. Au-delà de son aspect vital dans l’Extrême-Nord du Cameroun, l’agropastoralisme est confronté aujourd’hui au phénomène des changements climatiques qui s’accentue de plus en plus. Les effets du changement climatique se traduisent par la faiblesse de la pluviométrie, des sécheresses par moment, mais aussi des inondations à répétition. Ayant modifié la pratique agropastorale, le changement climatique a poussé les acteurs et actrices du développement local durable à adopter des stratégies de résilience sans lesquelles l’économie de la région serait menacée. L’objectif de cet article est d’analyser les différentes stratégies d’adaptation au changement climatique développées au niveau local par les populations et les partenaires au développement. Au-delà de l’adaptabilité des populations, la vulnérabilité de l’agropastoralisme face au changement climatique dans le septentrion camerounais crée souvent des tensions qui se transforment en conflit ouvert ou latent et menacent ainsi le vivre-ensemble.
THE MODERATING ROLE OF RELIGIOSITY ON THE RELATIONSHIP BETWEEN SATISFACTION WITH WORK-LIFE BALANCE AND JOB SATISFACTION
Authors:   University of Balochistan, Quetta., Karim Jahanvash
Published: March 2021

Purpose: - The impact of work-life balance on work-related outcomes has been the focus of much attention by researchers in organizational behavior and psychology. The aim of this study was to extend this literature by examining the potential moderating effect of religiosity on the relationship between satisfaction with work-life balance and job satisfaction. Methodology/Sample: - Data from The European Social Survey (ESS Round 6 Edition 1.2) provided the basis for this study (N= 21621). Findings: - Results suggested that satisfaction with work-life balance was positively related to job satisfaction and religiosity fostered the effect of satisfaction with work-life balance on job satisfaction. Practical Implications:- The findings of this study suggest that religiosity holds relevance to a variety of work outcomes. Faith and involvement in religious activities act as a personal resource, making individuals more resilient to cope with the challenges arising from work-life imbalance.
INFLUENCE OF PRODUCT PLACEMENT ON CONSUMER BEHAVIOR: THE MEDIATING ROLES OF CONSUMER RECOGNITION WITH PROGRAM CELEBRITY AND ETHICAL PERCEPTIONS TOWARDS PRODUCT PLACEMENT THAT LEADS TO CONSUMER PURCHASE INTENTION
Authors:   University of Engineering and Technology, Lahore., Khan Asad Ullah
Published: March 2021

Purpose: Product Placement is nowadays popular tool of advertisement. It is very difficult to place the product and synchronize it with the natural environment so that the consumer is acknowledged and has positive vibes towards the placed products brand. Objectives of this Research Article are that we have to find relation between Product Placement, Consumer Perception towards ethics of Product Placement, Consumer Recognition with Program Celebrity that leads to Consumer Purchase Intention. Methodology/Sampling: The study is based on 600 consumers who take part in the study and Quantitative Research methods are used to analyze the results. SPSS 20.0 is used for the purpose of data analysis in this study. Findings: The results showed that there is a positive relationship between these variables Product Placement, Consumer Perception towards ethics of Product Placement, Consumer Recognition with Program Celebrity that leads to Consumer Purchase Intention and Consumer Purchase Intention. Practical Implication: This study recommends Brands and their Product Placement strategists to follow the ethical approach in placing their Product in the movie or Television series use the Celebrity attachment with the Product in a way that there is a Positive effect on the Consumer and our goal to enhance Consumer Purchase Intention is accomplished.
IMPACT OF SAFETY HEALTH ENVIRONMENT ON EMPLOYEE RETENTION IN PHARMACEUTICAL INDUSTRY: MEDIATING ROLE OF JOB SATISFACTION AND MOTIVATION
Authors:   University of Engineering and Technology, Lahore, Pakistan., Salman Saad
Published: March 2021

Purpose: This study analyzes how employee retention is influenced by factors like safety health environment, job satisfaction and motivation. Methodology/Sampling: This research is quantitative in nature, where 250 survey questionnaires have been distributed within the employees of pharmaceutical industry in Pakistan. SPSS and AMOS have been utilized to analyze the direct and mediating effect of variables. Findings: This study confirms that employee retention is a significant outcome of safety health environment. Moreover, the levels of job satisfaction and employee motivation equally mediate the relationship between safety health environment and employee retention. Practical implications: The findings of this paper suggest that the pharmaceutical firms in Pakistan and specifically in Lahore should foster the health protection policies, and in turn it would help enhance the satisfaction and motivation of staff, resulting in the retention of capable employees.
DETERMINANTS OF TEACHERS’ TURNOVER INTENTION IN BAHRIA SCHOOLS AND COLLEGES AT KARACHI
Authors:   Bahria University, Karachi., Khan Rafique Ahmed
Published: March 2021

Purpose: This paper basically aims at exploring and investigating the impact of factors including career growth opportunities, workplace environment, managerial support, rewards & recognition system , and work-life balance on ‘turnover intention’ of teachers, in Bahria Schools and Colleges at Karachi. For the sake of ascertaining influence of these factors on employee turnover intention, six Bahria Schools and Colleges at Karachi were chosen. Methodology: By adopting a quantitative approach, views of 120 respondents (both male & female) were obtained with the help of a well structured questionnaire. The data were treated by applying correlation and regression analysis tools with the help of SPSS software, version 22. Findings: The results indicated that all five factors were negatively related with teachers’ turnover intention. However, impact of work-life balance was not insignificant. Similarly, results of the female employees varied from those of the male respondents. Male employees were comparatively less concerned about the issues related to work-life balance as compared to their female counterparts. Since all independent variables, if suitably practiced, also lead to employee satisfaction, the study clearly reflected the inverse relationship between employee satisfaction and turnover intention. Implications: This study can help the management of Bahria Schools and Colleges to understand the factors responsible for employee turnover intention. By paying due attention towards the identified factors, the Bahria Schools and Colleges can significantly enhance the motivation level of teachers, thereby reducing their turnover intention. The study is considered equally useful for other Schools and Colleges. In the end, future researchers have been advised to further explore the likely contribution of other factors towards employee satisfaction and reduced turnover intention.
TROFEJNE KARAKTERISTIKE SRNDAĆA (CAPREOLUS CAPREOLUS L.) U RAZLIČITIM LOVIŠTIMA SRBIJE
Authors:   University of Belgrade, Faculty of Agriculture, Davidovic Vesna , Popovic Zoran , Perisic Predrag , Slijepcevic Goran , Stojanovic Bojan , Božičkovic Ivana
Published: March 2021

The aim of this paper was to investigate and compare the value of morpho-metric parameters of antlers and the total score of 1175 roe deer trophies from nine hunting ground on the territory of the Republic of Serbia. The largest average length of the antlers was recorded in the hunting ground "Takovo" and it is 27.7 cm. We notice that the largest mass of antlers was measured in the hunting ground "Barajevska reka", which is 572 g. The smallest volume of antlers in roe deer was measured in the hunting ground "Krilaš" which is 15 cm³, then the hunting ground "Gučevo" where recorded volume of antlers was 18 cm³. The highest average value of the range of antlers of 12.62 cm is found in roe deer shot in the hunting ground "Mojsinjske planine" with the maximum recorded range of antlers of 19 cm and the minimum of 6.9 cm. Roe deer shot in the hunting ground "Takovo" had the lowest average grade of the trophy, only 62.23 CIC points.
ISPITIVANJE VREDNOSTI POKAZATELJA ENERGETSKOG I PROTEINSKOG STATUSA MLEČNIH KRAVA
Authors:   University of Belgrade, Faculty of Agriculture, Davidovic Vesna , Stojanovic Bojan , Perisic Predrag , Aleksic Slavica , Bozickovic Ivana , Relic Renata
Published: March 2021

The aim of this study was to examine the values of energy and protein status indicators of Holstein Frisian dairy cows in different production periods (20 days before calving, 30th and 90th day of lactation period). The study included 76 clinical healthy cows, which were divided into 2 groups. The first group consisted (n = 38) of pregnant heifers (or cows in first lactation after calving), and the second group (n = 38) consisted of cows calved multiple times (cows from second to fourth lactation). The results indicate that average concentration of glucose, albumins and urea at both groups in all phases of investigation are not significantly different in tested specimens (p>0.05). β-hydroxybutyrate concentration (BHBA) at pregnant individuals in late pregnancy period was statistically lower than 30th and 90th day of lactation, with p<0.05 at first and p<0.01 at second group. Lower values of total proteins at both groups were recorded 20 days before calving relative to lactation period, with statistical signifikante at p<0.01 for the first and p<0.05 for the second group. Heifers’ blood globulin concentration was significantly lower (p<0.05) relative to values determined after calving, during lactation period. Multiparous cows before calving also had lower average values of globulin, but differences were not significant.
ZAGAĐENJE ŽIVOTNE SREDINE I ZDRAVLJE ČOVEKA
Authors:   University of Kragujevac, Faculty of Agronomy, Đukic Dragutin , Mandic Leka ,   University of Kragujevac, Faculty of Agronomy, Đurovic Vesna ,   University of Kragujevac, Faculty of Agronomy, Semenov Aleksandar ,   Lomonosov Moscow State University, Moscow, Veskovic Slavica ,   Institute for Hygiene and Meat Technology, Belgrade, Serbia, Stojanov Monika ,   Faculty of Agricultural Sciences and Food, Skopje, Northern Macedonia, Mladenovic Jelena ,   University of Kragujevac, Faculty of Agronomy
Published: March 2021

The paper discusses: ecological conditionality of human pathology in conditions of environmental pollution; basic concepts and methods of testing in the system "human health  environment"; some of the most famous environmental diseases.
TOKSIKOLOŠKI IZVEŠTAJ O TROVANJU PESTICIDIMA U JUGOISTOČNOM REGIONU SRBIJE TOKOM 2020. GODINE
Authors:   University of Niš, Faculty of Medicine Niš, Serbia, Kostic Emilija , Vujovic Maja
Published: March 2021

Pesticides are substances widely used in agriculture, which are the cause of acute poisoning in a significant percentage worldwide. The aim of this paper is to analyze cases of pesticide poisoning during 2020, according to the data of the Toxicology Laboratory of the Institute of Forensic Medicine in Nis. Pesticide poisoning has been proven in 18 cases (8.11% of all cases). Organophosphate pesticides were detected in nine cases, glyphosate in five, pendimethalin in two, while metolachlor and piperonyl butoxide were detected in one case each. Data on cases of poisoning indicate that education on the proper use of pesticides is necessary in order to reduce the number of acute and chronic poisonings.
POTENCIJAL BIOAKUMULACIJE I TRANSLOKACIJE METALA KOD VRSTE ACHILLEA MILLEFOLIUM SA RAZLICITIH LOKALITETA
Authors:   University of Kragujevac, Faculty of Science Kragujevac, Đelic Gorica , Simic Zoran ,   University of Kragujevac, Faculty of Science Kragujevac, Brankovic Snezana ,   University of Kragujevac, Faculty of Science Kragujevac, Stankovic Milan ,   University of Kragujevac, Faculty of Science Kragujevac, Pavlovic Milica ,   University of Kragujevac, Faculty of Science Kragujevac, Jakšic Tatjana ,   University of Pristina Faculty of Science, Kosovska Mitrovica, Vasic Predrag ,   University of Pristina Faculty of Science, Kosovska Mitrovica
Published: March 2021

Achillea millefolium L. has a great value as medicinal plant. The aim of the research is to determine the uptake potential for heavy metals and the intraspecial difference between unique species of A. millefolium from different localities based on the heavy metals uptake, their distribution in plant organs and enrichment factors. The results showed that plants growing on soil rich in heavy metals (tailings) absorb significantly higher amounts of metals compared to plants from the Ovčar-Kablar gorge (non-contaminated soil).
ALERGENE BILJKE NA TERITORIJI GRADA KRAGUJEVCA
Authors:   University of Kragujevac, Faculty of Science Kragujevac, Đelic Gorica , Stankovic Milan , Bojovic Biljana , Pavlovic Milica
Published: March 2021

The paper presents the quantitative and qualitative characteristics of allergenic plants in the urban area of the Kragujevac city. The ecological characteristics of these plants were determined through ecological index and life forms. During the conducted floristic study, the presence of 78 allergenic plants was determined. Half of the recorded allergenic plant species are woody ornamental plant species. Floristic analysis shows that the allergenic plants are from the Pinophyta and Magnoliophyta divisions from 19 families, most commonly conifers and grasses.
ZNAČAJ MIKROBIOLOŠKE ISPRAVNOSTI VODE U ZAŠTITI ZDRAVLJA STANOVNIŠTVA
Authors: Šarčevic - Todosijevic Ljubica , Đorđevic Snezana , Popovic Vera , Živanovic Ljubisa , Petrovic Bojana , Đorđevic Nikola , Stevanovic Aleksandar
Published: March 2021

In addition to physical and chemical pollution of water, from the aspect of the impact of water on the health of the population, monitoring and prevention of microbiological pollution of water, especially water used for drinking, is extremely important. In this paper, the importance of microbiological safety of water in protecting the health of the population is considered.
UTICAJ OTPADA NA FIZIČKO-HEMIJSKE KARAKTERISTIKE ZEMLJIŠTA
Authors:   University Edukons, Serbia, Stojic Natasa , Pucarevic Mira ,   University Edukons, Serbia, Živkovic Milica ,   University of Novi Sad, Faculty of Science, Teofilovic Vesna ,   Univerzitet u Novom Sadu, Tehnološki fakultet, Prokic Dunja ,   University Edukons, Serbia
Published: March 2021

Land exploitation directly affects the physico-chemical characteristics of the soil. Agricultural land can often be seen near the landfills. The question is how much waste affects the production potential of land. The research in this paper is focused on the influence of the municipal waste landfill on the physico-chemical characteristics of the soil. In soil samples were analyzed: pH in KCl, pH in H2O, CaCO3, humus, total nitrogen, P2O5, K2O and organic carbon. The obtained results indicate a negative impact of waste on the content of OC and the content of easily accessible phosphorus and potassium.
DIVERZITET MAKROMICETA PLANINE ,,BUKULJA“
Authors:   University of Kragujevac, Faculty of Science Kragujevac, Lačkovic Nikola , Rankovic Branislav , Kosanic Marijana , Petrovic Nevena
Published: March 2021

The main purpose of this study is showing the diversity of the world of macromycetes of the mountain Bukulja (central Serbia). Field studies were conducted for five years and resulted in discovering 80 species of macromycetes. Many of identified species are rare or legally protected. Considering that there were not any similar studies regarding the diversity of macromycetes of this mountain, this study can be contemplated as an unique contribution to the knowledge about the diversity of macromycetes of this area and wider, the diversity of macromycetes of Serbia.
SISTEM ZA PREČIŠĆAVANJE PIJAĆE VODE SA POVEĆANOM TVRDOĆOM I KONCENTRACIJOM AMONIJAKA, GVOŽĐA, MANGANA 
Authors:   University of Kragujevac, Faculty of Agronomy, Trisovic Tomislav , Rafailovic Lidija , Li Wei ,   School of Mathematics and Statistics, Xidian University, Xi'an, China;, Grgur Branimir , Trisovic Zaga
Published: March 2021

Water is one of the most important universal solvents for gerat number of substances. Despite the fact that it is found in nature in huge quantities, there is almost no completely clean water in nature. The purest water in the nature is in glaciers, snow and possibly rainwater, although this water contains also a certain amount of impurities. Water in natural springs (underground and surface springs) is more or less loaded with minerals that can be non-toxic or very toxic. In order to use it as drinking water, it is necessary that such water (chemically and bacteriologically) be in accordance with the standards and Guidance which defines the maximum allowed concentrations of certain mineral compounds and bacteria in drinking water.
EFEKAT PRAJMIRANJA SEMENA PŠENICE (Triticum aestivum L.) NA SADRŽAJ FOTOSINTETSKIH PIGMENATA I UKUPNIH SOLUBILNIH PROTEINA
Authors:   University of Kragujevac, Faculty of Science Kragujevac, Bojovic Biljana , Kanjevac Milica , Jakovljevic Dragana
Published: March 2021

In this paper, effect of different priming treatments in the pregerminative phase of wheat seeds (Triticum aestivum L.) on the concentration of photosynthetic pigments and total soluble proteins in the leaf of seedling was investigated. Seeds were treated with solutions of the phytohormones gibberellin and auxin (hormone priming), salts of potassium and magnesium (halo priming), ascorbic acid and hydrogen peroxide (chemo priming) and water (hydro priming). Based on the obtained results, it was determined that the content of pigments and total soluble proteins can be increased by applying the appropriate priming treatment. The most favorable effect on the examined parameters was observed in the treatment with potassium nitrate.
ODREĐIVANJE HEMIJSKOG SASTAVA RAZLIČITIH EKSTRAKATA ČUVARKUĆE
Authors:   University of Kragujevac, Faculty of Agronomy, Mladenovic Jelena , Markovic Nebojsa , Boskovic-Rakocevic Ljiljana , Đuric Milena , Pavlovic Nenad
Published: March 2021

Sempervivum tectorum has a similar effect as aloe vera, which is known in the treatment of various skin diseases. This herb is considered one of the safest remedies for a wide range of skin diseases. Due to its anti-inflammatory and antiseptic properties, it also serves as an excellent first aid for burns, stings and bites, because it provides quick relief and calming. Freshly squeezed juice from the leaves of the houseplant is used in the treatment of nervous disorders, epilepsy and restless dreams. The leaves are edible and can be used as an addition to salads or stews. They are not particularly tasty, but as they are rich in water, they can be put in a juicer together with other fruits or vegetables and become a refreshing drink. It is used in folk herbal medicine and as a medicine. The aim of this study was to determine the moisture content, total extracted substances, extract density, vitamin C, organic acids and proteins in house extracts.
ANTIRADIKALSKI KAPACITET (E)-N’-1-(2,4-DIOKSO-2H-HROMEN-3(4H)-ILIDENE)ETIL)-4-HIDROKSI-3-METOKSIBENZOHIDRAZIDA
Authors:   University of Kragujevac, Institute for Information Technologies, Antonijevic Marko , Milenkovic Dejan , Kesic Ana , Avdovic Edina , Markovic Zoran
Published: March 2021

Coumarins are a widespread group of compounds. They have a wide range of physiological effects, from antifungal, through anticoagulant to anticancer, and therefore they have found wide application in the treatment of various diseases. In this paper, the ability of 4-hydroxy-3-methoxy-N'-((E)-1-(2,4-dioxo-2H-chromen-3(4H)-ylidene)ethyl)benzohydrazide to inactivate ROS was investigated. The analysis of the obtained results showed that the SET-PT (Single Electron Transfer Followed By Proton Transfer) mechanism is non-operative. On the other hand, the thermodynamic parameters describing the HAT *Hydrogen Atom Transfer) and the first step of the SPLET (Sequential Proton Loss-Electron Transfer) mechanism indicate that both mechanisms will be operative. The antiradical capacity of the test compound with the radicals decreases in the following order: •OH> •OCH3> •OC(CH3)3> •OOH> •OOCH3> •OOCH2CH3.
UTICAJ NAČINA PRESOVANJA NA PROMENE LEŠNIKOVOG ULJA TOKOM ČUVANJA I SENZORNA SVOJSTVA KEKSA
Authors:   University of Kragujevac, Faculty of Agronomy, Radovanovic Mirjana , Petkovic Marko , Đurovic Vesna , Miletic Nemanja , Rumenic Katarina
Published: March 2021

The changes in the spectrum, peroxide numbers and specific absorbances (at 232 nm and 270 nm) of fresh and storaged oils (4 months at 4 °C) obtained by different hazelnuts pressing were monitored. The results showed reduced viability of the oil obtained from the hazelnut exposed to elevated temperature during conditioning and pressing: changes in the spectrum about 290 nm, a peroxide number close to the legally allowed maximum (7.5 mmolkg-1) and possible presence of secondary oxidation products. Sensory evaluation of biscuits with hazelnut oil showed that the samples belonged to the categories of quality: very good and good. The overall sensory score was highest for biscuits with hazelnut oil pressed in an electric press.
THE INFLUENCE OF TWO STARTER CULTURES ON THE MICROBIOLOGICAL STABILITY OF MACEDONIAN TRADITIONAL SAUSAGE
Authors:   University of Ss. Cyril and Methodius, Faculty of Agricultural Sciences, Skopje, Stojanova Monika , Najdenovska Olga ,   University of Ss. Cyril and Methodius, Faculty of Agricultural Sciences, Skopje, Đukic Dragutin ,   University of Kragujevac, Faculty of Agronomy
Published: March 2021

The aim of this paper is to monitor the influence of two starter cultures on the microbiological stability of Macedonian traditional sausage. The research covered three variants: Control variant; Variant 2: with addition of starter culture CS-300; Variant 3: with addition of starter cultures CS-300 and BLC-78. The total bacteria count and Lactobacillus sp. in all three variants decreases compared to the initial value. There is no presence of Escherichia coli and Enterobacteriaceae.Starter culture CS-300 is recommended, while better stability of the microflora is achieved during the storage period, as well as a good quality. At the same time, the use of nitrite salt is eliminated, which results in getting a safe product.
PREGLED REZULTATA KISELOSTI SIROVOG MLEKA NA TERITORIJI OPŠTINE SJENICA
Authors:   Regional center for development of agriculture and villages, Serbia, Cucevic Nedim , Koprivica Ranko ,   University of Kragujevac, Faculty of Agronomy, Bibic Mejrema ,   Regional center for development of agriculture and villages, Serbia, Prelic Anida ,   Regional center for development of agriculture and villages, Serbia, Hodžic Esad ,   Regional center for development of agriculture and villages, Serbia, Masovic Jasmina ,   Regional center for development of agriculture and villages, Serbia, Salakovic Benjamin ,   University of Novi Sad, Faculty of Technology
Published: March 2021

The aim of the work is to determine the acidity (pH) of milk produced on family farms in the Municipality of Sjenica depending on the season and month of production. It found an analysis of 3,226 milk samples that manufacturers and processors brought voluntarily to the lab. The process of receiving and analyzing the samples was done according to the Rulebook on the Quality of Raw Milk and ISO/IEC 17025:2017. The number of samples of raw milk in winter (529) is lower than in summer (1094). The average pH is the highest in the month of December (6.70), and the lowest in March (6.52). On the territory of the Municipality of Sjenica there was a steady acidity of milk per month in 2019. and moved within the boundaries envisioned in the Regulation on the Quality of Raw Milk.
VARIJABILNOST SESKVITERPENA ETARSKIH ULJA VRSTE TEUCRIUM MONTANUM L.
Authors:   University of Kragujevac, Faculty of Science Kragujevac, Zlatic Nenad , Mihailovic Vladimir , Đelic Gorica , Ljesevic Marija , Beskoski Vladimir , Stankovic Milan
Published: March 2021

The aim of this study is to determine the qualitative variability and relative presence of sesquiterpene hydrocarbons in essential oils samples of Teucrium montanum L. collected from two different substrate types. Four samples of essential oils from serpentinite and four from calcareous were obtained by the method of hydrodistillation from aboveground plant parts. Based on the results, it was shown that populations from serpentinite habitats synthesize 2 times more sesquiterpenes than from calcareous ones, while the compound 6-epi-β-cubeben was detected only in populations from calcareous. Edaphic characteristics of habitats affect the qualitative composition and relative presence of sesquiterpenes in essential oils, which has a significant application in biotechnology.
HEMIJSKI SASTAV I SENZORNA SVOJSTVA FRANKFURTERA SA BILJNIM ULJIMA
Authors:   University of Belgrade, Faculty of Agriculture, Stajic Slavisa , Zivkovic Dusan
Published: March 2021

Emulsified sausages (e.g. frankfurters, mortadella) are well-known and widely consumed meat products. The aim of this experiment was to examine the impact of the substitution of various proportions of fatty tissue (20%, 60% and 100%) with emulsions (with soy protein isolate) of grapeseed and pumpkin seed oil on the chemical composition and sensory characteristics of frankfurters. We determined that the higher share of plant oils in the mixture led to a progressive increase in the moisture and protein contents, whereas the fat content decreased. The type and amount of oil in the batches had a significant impact on sensory evaluation results, with pumpkin seed oil having a more pronounced impact – frankfurters where 100% of the fatty tissue was substituted with pumpkin seed oil emulsion were unacceptable for consumers.
UTICAJ ODREĐENIH STRESOGENIH FAKTORA NA KVALITET GOVEĐEG MESA
Authors:   University of Kragujevac, Faculty of Agronomy, Kurcubic Vladimir , Stajic Slavisa ,   University of Belgrade, Faculty of Agriculture, Miletic Nemanja ,   University of Kragujevac, Faculty of Agronomy
Published: March 2021

Stress inevitably occurs from farm to slaughter in modern cattle breeding. The effects of stress on behavior, physiological status, and meat quality have been examined by a number of authors. The mechanism of the effects of stress on physiological and biochemical changes and the consequent effects on meat quality attributes are significantly more limited. This review summarizes the primary stress factors that affect animal welfare and cause biochemical changes during the early postmortem period, which reduces the quality of carcasses and their nutritional quality. In order to obtain high quality meat, further studies are needed to uncover the complex mechanisms mentioned above.
ANTIOKSIDATIVNI KAPACITET ANTRAHINONA IZ BILJKE RUBIA CORDIFOLIA LINN
Authors:   University of Kragujevac, Institute for Information Technologies, Milanovic Žiko , Antonijevic Marko , Kesic Ana , Dimic Dusan , Đorovic Jovanovic Jelena
Published: March 2021

In this study, the antioxidant and antiradical capacities of anthraquinones from Rubia cordifolia Linn were determined using the DFT method. The thermodynamically most favorable reaction pathways of antioxidant mechanisms were defined, as well as the most favorable antiradical reaction mechanisms for the deactivation of methylperoxyl and methoxy radicals. The calculations were done in the gas phase. HAT is thermodynamically the most favorable mechanism of antioxidant action of the studied anthraquinones, while the most probable mechanism of deactivation of methylperoxyl and methoxy radicals is the SPLET mechanism.
A correlational study on physical and mental status of lived experience of hospitalised COVID-19 patient
Authors: Kadu Amit , Karandikar Dr. Neeraj J. , Bhattacharya Smt. Chanu
Published: March 2021

Introduction: Some of the public health measures necessary to contain the spread of the pandemic, such as quarantines and social distancing, increase the risk of adverse psychological consequences. A recent review revealed that these risks are greater with longer quarantine periods, when there are infection fears, frustration, boredom, inadequate supplies, inadequate information, and financial loss. COVID-19 pandemic to have disproportionately adverse impacts on mental health of hospitalized groups. COVID-19 is a disease, impact physically but it has been affecting much more the mental health of patient.  Aim & Objectives: To Assessment of physical status and mental status of patient during hospitalization and the correlation. To find out the relationship between selected socio-demographic status Age, gender, race, ethnicity, employment, insurance, education, marital status, psychiatric therapies, health literacy, social support, financial distress with the level of mental status. Methods: A cross sectional study with one centre data, collected with selected criteria. Fifty participants were selected for collection of livid experience expressed in local language. Study approach was mix method and Qual-quan design. Assessed by experts and evaluators. Quantitative data collected with checklist and socio demographics detail were collected from patient. Results: Result showed Qualitative themes clearly showed the mental state of patient like “I will die”. Quantitatively Physical status is significantly corelated with mental status, there is a significant statistical co-dependency assessed by Pearson’s product moment test. Socio-demographic data age as chai square value physical status (4.54) as mental status (4.59), marital status (5.25) and social support (4.87) is significantly associated with mental status, test done in 0.05 level of significance. Conclusion: Though one centre data is a limitation of the study still it can be visible that COVID -19 patients need mental healing or therapy parallelly with medicines. The mental symptoms should not be secondary cure it must be primary for healing of body.
Unstable trochanteric Fracture Fixation with P.F.N. augmented by T.B.W.
Authors: Patil Dr. Vijay , Daule Dr. Pandurang , Naikwade Dr. Deepak
Published: March 2021

Biomechanically Proximal Femoral Nail (PFN) is a better choice of implant. Still it is associated with screw breakage, cut out through femoral head,”Z” effect, reverse “Z” effect and lateral migration of screws. The purpose of this study is to evaluate the results of augmented PFN in terms of post operative complication and failure rates in unstable trochanteric fracture. Methodology: We did study of 21 unstable trochanteric fractures from Jan 2016 to Nov .2020. 14 patients were males & 7 patients were females. Age group between 25 to 80 was included in this group. There were 15 A0 A2 (2.2, 2.3) and 6 were A0 A3 (3.1, 3.2, 3.3). All fractures were fixed with 25cm, 1350 PFN mainly 11 mm, 12mm in diameter augmented with one or two 16 gauge tension band wire, strengthening lateral trochanteric wall and holding either communiated   fragments or lesser trochanteric fracture. Results: The bone healing was observed in all cases with mean period of 16 weeks. Two patients developed complication of TBW breakage; one developed lateral migration of screws. Patients were followed till # union. At the end of follow up the Salvati and Wilson hip function was 30 (out of 40) in 80% patients. Conclusion: The stabilization of lateral trochanteric wall fracture and communicated fragments with additional one or two TBW increases the stability of construct enhancing bony union & better results.