bluuebunny/crossref_metadata_2025 huggingface dataset:
Flow Topology of the Bi-Stable Wake States for the DrivAer Fastback Model
Authors: Aultman Matthew , Duan Lian
Published: April 2024

For this work, conditional averaging and Proper Orthogonal Decomposition (POD) were used to analyze the salient three-dimensional structures in the wake of a DrivAer fastback model with smooth underbody. Conditional averaging revealed that the bi-stable structure of the wake consists of a ring-like structure with three vortex legs, which includes a vortex pair on the side associated with the bi-stability and one on the opposite side associated with the wheel vortex. POD revealed the entrainment of low-momentum fluid from the wheel wake into the vortex pair leads to an induced spanwise crossflow which drives a feedback loop for the bi-stability. The resultant bi-stable structure was dependent on the state of the wheels. With stationary wheels, the feedback mechanism is enhanced, leading to higher spanwise crossflow that breaks the ring-like vortex. A different structure was observed when the wheels rotate, wherein the ring-like structure is unbroken and pierced by the vortex pair. The feedback mechanism and resultant vortex structure are similar to those found in simplified square-back models. Given the similarity in bi-stability between realistic and simplified vehicles, the suppression of the bi-stability in realistic vehicles could initially be based on the same mechanism as that for simplified square-back models.
Continued decline in the incidence of myocardial infarction beyond the COVID-19 pandemic: a nationwide study of the Swedish population aged 60 and older during 2015–2022
Authors: Meyer Anna C. , Ebeling Marcus , Acosta Enrique , Modig Karin
Published: April 2024

The number of myocardial infarctions declined during the early COVID-19 pandemic but mechanisms behind these declines are poorly understood. COVID-19 infection is also associated with an increased risk of myocardial infarction which could lead to higher incidence rates in the population. This study aims to shed light on the seemingly paradoxical relationship between COVID-19 and myocardial infarction occurrence on the population level by exploring long-term trends in incidence rates, case fatality, and proportion of patients dying before reaching a hospital. Our work is based on a linkage of administrative registers covering the entire population aged 60 + in Sweden. Considering both long-term trends since 2015 and seasonal variability, we compared observed incidence, case fatality, and proportions of patients hospitalized to expected values during 2020–2022. Despite more than 200 laboratory-confirmed COVID-19 cases per 1000 inhabitants by the end of 2022, incidence rates of myocardial infarction continued to decline, thus following the long-term trend observed already before 2020. During the first pandemic wave there was an additional incidence decline corresponding to 13% fewer myocardial infarctions than expected. This decline was neither accompanied by increasing case fatality nor by lower shares of patients being hospitalized. We found no increase in the population-level incidence of myocardial infarction despite large-scale exposure to COVID-19, which suggests that the effect of COVID-19 on myocardial infarction risk is not substantial. Increased pressure on the Swedish health care system has not led to increased risks or poorer outcomes for patients presenting with acute myocardial infarction.
Limiting Access to Certain Anonymous Information: From the Group Right to Privacy to the Principle of Protecting the Vulnerable
Authors: Asgarinia Haleh
Published: April 2024

An issue about the privacy of the clustered groups designed by algorithms arises when attempts are made to access certain pieces of information about those groups that would likely be used to harm them. Therefore, limitations must be imposed regarding accessing such information about clustered groups. In the discourse on group privacy, it is argued that the right to privacy of such groups should be recognised to respect group privacy, protecting clustered groups against discrimination. According to this viewpoint, this right places a duty on others, for example, private companies, institutions, and governments, to refrain from accessing such information. To defend the idea that the right to privacy should be recognised for clustered groups, at least two requirements must be satisfied. First, clustered group privacy must be conceived of as either a collective good or a participatory good. Since these forms of good are of the type from which no member of a group can be excluded from benefiting, the right to them is defined as a group right. Second, there must be group interests on which to base a group right. Group interests can be either the interests of those members that are a result of their being in the group or the interests of the group as a whole that transcend the interests of its members. However, this paper argues that clustered group privacy cannot be conceived of as either a collective or a participatory good because it is possible for some individuals to be excluded from benefiting from it. Furthermore, due to the lack of awareness among individuals that they are members of a clustered group and the nature of a clustered group itself, such groups cannot have the group interests necessary to establish a group right. Hence, the group right to privacy cannot be recognised for these groups, implying that the group right cannot be considered a means to protect clustered groups against discrimination. Instead, this paper suggests that moral principles need to be articulated within an ethics of vulnerability to identify the moral obligations of protecting vulnerable clustered groups. The duty owed to the vulnerable should involve refraining from accessing certain information about clustered groups in specific contexts. This duty is not engendered by the right to privacy of such groups; it is the duty owed to the vulnerable. The findings highlight the need to articulate moral principles regarding privacy and data protection to protect clustered groups in contexts in which accessing information about them could constitute a reason for discriminatory targeting.
The characteristics of white dot syndromes following COVID-19 Vaccines: a systematic review
Authors: Abu Serhan Hashem , Abu Suilik Husam , Hassan Amr K. , AlSamhori Jehad Feras , Hassan Abdul Rhman , Siddiq Abdelmonem , Ahmed Nagi , Elnahry Ayman G.
Published: April 2024

Purpose

To review all studies reporting the onset of white dot syndromes following COVID-19 vaccines.

Methods

Our protocol was registered prospectively on PROSPERO [registration number: CRD42023426012]. We searched five different databases including PubMed, Scopus, Web of Science, Google Scholar, and Science Direct up to May 2023. All the studies that reported the occurrence of white dot syndrome following COVID-19 vaccines were included. All statistical tests were conducted with a 95% confidence interval and a 5% error margin. Apvalue of less than 0.05 was considered statistically significant. The methodological quality of included studies was performed using the IHE Quality Appraisal Checklist for Case Series studies and JBI Critical Appraisal Checklist for Case Reports.

Results

Fifty studies involving seventy-one subjects were included. Multiple evanescent white dot syndrome (MEWDS) was the most common disease (n = 25, 35.2% %), followed by acute macular neuroretinopathy (AMN) (n = 22, 31.0%) and acute posterior multifocal placoid pigment epitheliopathy (APMPPE) (n = 4, 5.6%). They were mostly unilateral (n = 50, 70.4%). The presenting symptoms were blurred vision (n = 26, 36.6%), paracentral scotoma (n = 19, 26.8%), visual field disturbance, and photopsia (n = 7, 9.9%). The mean duration for follow-up was 10.15 ± 14.04 weeks. Nineteen subjects (29.69%) received steroids with improvement reported in 68.4%. Eleven subjects (17.19%) were managed by observation only with reported full recovery and improvement.

Conclusion

White dot syndromes are very rare entities. Our findings highlight a possible association between COVID-19 vaccines and the occurrence of white dot syndromes. However, larger studies with good quality should be implemented to confirm these findings.
Dissolution Profiles of Immediate Release Products of Various Drugs in Biorelevant Bicarbonate Buffer: Comparison with Compendial Phosphate Buffer
Authors: Okamoto Nanami , Higashino Masaki , Yamamoto Hibiki , Sugano Kiyohiko
Published: April 2024

Purpose

The purpose of this study was to clarify the extent to which the dissolution profiles of immediate release (IR) products of various drugs differ between biorelevant bicarbonate buffer (BCB) and compendial phosphate buffer (PPB).

Methods

The dissolution profiles of the IR products of fifteen poorly soluble ionizable drugs were measured in BCB and PPB. BCB was set to be relevant to the small intestine (pH 6.8, 10 mM). The pH was maintained using the floating lid method. The Japanese pharmacopeia second fluid (JP2, 25 mM phosphate buffer, nominal pH 6.8) was used as compendial PPB. The compendial paddle apparatus was used for the dissolution tests (500 mL, 50 rpm, 37°C).

Results

In 11/15 cases, a difference in dissolved% (< 0.8 or > 1.25-fold) was observed at a time point. In 4/15 cases, the ratio of the area under the dissolution curve was not equivalent (< 0.8 or > 1.25-fold). In the cases of free-form drugs, the dissolution rate tended to be slower in BCB than in JP2. In the case of salt-form drugs, a marked difference was observed for the cases that showed supersaturation. However, no trend was observed in the differences.

Conclusions

Many IR products showed differences in the dissolution profiles between biorelevant BCB and compendial PPB. With the floating lid method, BCB is as simple and easy to use as PPB. Biorelevant BCB is recommended for dissolution testing.
Diagnostic and therapeutic use of oral micronized progesterone in endocrinology
Authors: Memi Eleni , Pavli Polina , Papagianni Maria , Vrachnis Nikolaos , Mastorakos George
Published: April 2024

Progesterone is a natural steroid hormone, while progestins are synthetic molecules. In the female reproductive system, progesterone contributes to the control of luteinizing hormone and follicle-stimulating hormone secretion and their pulsatility, via its receptors on the kisspeptin, neurokinin B, and dynorphin neurons in the hypothalamus. Progesterone together with estradiol controls the cyclic changes of proliferation and decidualization of the endometrium; exerts anti-mitogenic actions on endometrial epithelial cells; regulates normal menstrual bleeding; contributes to fertilization and pregnancy maintenance; participates in the onset of labor. In addition, it exerts numerous effects on other endocrine systems. Micronized progesterone (MP) is natural progesterone with increased bioavailability, due to its pharmacotechnical micronized structure, which makes it an attractive diagnostic and therapeutic tool. This critical literature review aims to summarize and put forward the potential diagnostic and therapeutic uses of MP in the field of endocrinology. During reproductive life, MP is used for diagnostic purposes in the evaluation of primary or secondary amenorrhea as a challenge test. Moreover, it can be prescribed to women presenting with amenorrhea or oligomenorrhea for induction of withdrawal bleeding, in order to time blood-sampling for diagnostic purposes in early follicular phase. Therapeutically, MP, alone or combined with estrogens, is a useful tool in various endocrine disorders including primary amenorrhea, abnormal uterine bleeding due to disordered ovulation, luteal phase deficiency, premenstrual syndrome, polycystic ovary syndrome, secondary amenorrhea [functional hypothalamic amenorrhea, premature ovarian insufficiency], perimenopause and menopause. When administrated per os, acting as a neurosteroid directly or through its metabolites, it exerts beneficial effects on brain function such as alleviation of symptoms of anxiety and depression, asw well as of sleep problems, while it improves working memory in peri- and menopausal women. Micronized progesterone preserves full potential of progesterone activity, without presenting many of the side-effects of progestins. Although it has been associated with more frequent drowsiness and dizziness, it can be well tolerated with nocturnal administration. Because of its better safety profile, especially with regard to metabolic ailments, breast cancer risk and veno-thromboembolism risk, MP is the preferred option for individuals with an increased risk of cardiovascular and metabolic diseases and of all-cause mortality.
Addressing Democracy and Its Threats in Education: Exploring a Pluralist Perspective in Light of Finnish Social Studies Textbooks
Authors: Mikander Pia , Satokangas Henri
Published: April 2024

Democracy is increasingly being challenged, by disengagement and by anti-pluralist movements (Levitsky and Ziblatt in How Democracies Die: What History Reveals About Our Future, Viking, New York, 2018; Wikforss in Därför demokrati. Om kunskapen och folkstyret [Because of this, democracy. On knowledge and people’s rule] Fri Tanke, 2021; Svolik et al. in J Democr 34(1):5–20, 2023). This article draws upon a theoretical discussion about democracy, pluralism, and threats to democracy. Departing from Dewey, Laclau, Mouffe, Young and Allen, we address democracy as an ideology that centers around pluralism, or an ever-increasing inclusion of voices from the margins as its goal. We argue that perceiving democracy pedagogically as a pluralistic ideology would support students’ democratic citizenship and equip them for a world where threats to democracy are being reported. Employing a case study on Finnish social studies textbooks, we analyze how democracy as well as threats to democracy are discursively portrayed. Our study shows that the textbooks present democracy as predominantly institutional and static. We also find that while disengagement is portrayed as a problem for democracy, anti-pluralist movements are generally not referred to as a threat. Additionally, we examine a discourse in the textbooks that connects freedom of speech with democracy in a way that favors a multitude of opinions, even antidemocratic ones, over creating space for marginalized voices. Drawing on the theoretical discussion and the results of the analysis, we argue that a focus on pluralism as the core of democracy makes the opposition between restricting hate speech and advocating for democracy redundant.
A combination of biomarkers for predicting stallion sperm fertility
Authors: Johannisson Anders , Morrell Jane M. , Ntallaris Theodoros
Published: April 2024

Equine breeding would benefit greatly from reliable biomarkers of stallion or ejaculate fertility. The aim of the study was to investigate how several in vitro sperm characteristics correlate with fertility after artificial insemination, to explore the potential to build a fertility prediction model for stallions. Cooled insemination doses (3–5 per stallion) were obtained from various studs. Sperm membrane integrity, acrosome integrity, chromatin integrity, mitochondrial membrane potential, and reactive oxygen species production were evaluated by flow cytometry 24–30 h after semen collection, and sperm motility was assessed by computer aided sperm analysis. Calcein violet was used to differentiate viable spermatozoa. Per season pregnancy rates for these stallions were available the following year. Positive correlations were found between pregnancy rate and straightness (r = 0.43, p ≤ 0.001), as well as pregnancy rate and the proportion of living hydrogen peroxide positive spermatozoa (r = 0.32, p ≤ 0.05). There were negative correlations between pregnancy rate and amplitude of lateral head displacement (r = -0.26, p ≤ 0.05), and between pregnancy rate and the mean fluorescence of dead superoxide positive spermatozoa (r = -0.46, p < 0.001). Principal component analysis indicated that motility, membrane integrity, DNA fragmentation, and reactive oxygen species production were associated with pregnancy rate. Therefore, a combination of these factors could be used as a biomarker of fertility when assessing ejaculates. However, data from more individuals would be required to construct a model for fertility prediction.
OntoMedRec: Logically-pretrained model-agnostic ontology encoders for medication recommendation
Authors: Tan Weicong , Wang Weiqing , Zhou Xin , Buntine Wray , Bingham Gordon , Yin Hongzhi
Published: April 2024

Recommending medications with electronic health records (EHRs) is a challenging task for data-driven clinical decision support systems. Most existing models learnt representations for medical concepts based on EHRs and make recommendations with the learnt representations. However, most medications appear in EHR datasets for limited times (the frequency distribution of medications follows power law distribution), resulting in insufficient learning of their representations of the medications. Medical ontologies are the hierarchical classification systems for medical terms where similar terms will be in the same class on a certain level. In this paper, we propose OntoMedRec, the logically-pretrained and model-agnostic medical Ontology Encoders for Medication Recommendation that addresses data sparsity problem with medical ontologies. We conduct comprehensive experiments on real-world EHR datasets to evaluate the effectiveness of OntoMedRec by integrating it into various existing downstream medication recommendation models. The result shows the integration of OntoMedRec improves the performance of various models in both the entire EHR datasets and the admissions with few-shot medications. We provide the GitHub repository for the source code. (https://github.com/WaicongTam/OntoMedRec)
What ratings and corpus data reveal about the vividness of Mandarin ABB words
Authors: Van Hoey Thomas , Yu Xiaoyu , Pan Tung-Le , Do Youngah
Published: April 2024

A well-known method of studying iconic words is through the collection of subjective ratings. We collected such ratings regarding familiarity, iconicity, imagery/imageability, concreteness, sensory experience rating (SER), valence and arousal for Mandarin ABB words. This is a type of phrasal compound consisting of a prosaic syllable A and a reduplicated BB part, resulting in a vivid phrasal compound, for example, wù-mángmáng 雾茫茫 ‘completely foggy’. The correlations between the newly collected ABB ratings are contrasted with two other sets of prosaic word ratings, demonstrating that variables that characterize ABB words in an absolute sense may not play a distinctive role when contrasted with other types of words. Next, we provide another angle for looking at ABB words, by investigating to what degree rating data converges with corpus data. By far, the variable that characterizes ABB items consistently throughout these case studies is their high score for imageability, showing that they are indeed rightfully characterized as vivid. Methodologically, we show that it pays off to not take rating data at face value but to contrast it with other comparable datasets of a different phenomenon or data about the same phenomenon compiled in an ontologically different manner.
Social Equality and Wrongful Discrimination: Introduction to the Special Issue on Moreau's Faces of Inequality
Authors: Cossette-Lefebvre Hugo
Published: April 2024

In this introduction, I briefly summarize Sophia Moreau's Faces of Inequality. I situate her monograph within two highly contemporary bodies of literature — relational egalitarianism and discrimination theory — to show how it provides important insights for understanding both what it means to treat others as equals in society and how to define wrongful discrimination. Moreau's work on discrimination is of great relevance for philosophers and socio-legal theorists alike as the commentaries from the symposium contributors demonstrate, including Dale Smith, Pablo Gilabert, Andrea Sangiovanni, Daniel Viehoff, Jessica Eisen, Alysia Blackham, and Iyiola Solanke.
Political Science as a Dependent Variable: The National Science Foundation and the Shaping of a Discipline
Authors: Moustafa Tamir
Published: April 2024

From 1965 to 2020, the National Science Foundation constituted the single largest funding source for political science research. As such, the NSF played a central role in defining the cutting-edge of our discipline. This study draws on historical records of the American Political Science Association to examine the political and administrative contexts that shaped the funding priorities of the NSF Political Science Program. Additionally, the study presents a new dataset and analysis of the nearly three thousand projects funded over the 55-year life of the program. The dataset shows that NSF funding was principally channeled toward quantitative research, whereas qualitative methods received little support, and work advancing normative, critical, or interpretive approaches received virtually no support. The archival record and awards-level data make visible the material forces that shaped knowledge production, and they underline the NSF’s instrumental role in consolidating behavioralism and marginalizing non-positivist approaches. The study sheds new light on the history of the discipline and helps to contextualize some of the distinctive features of American political science.
Lakes Worldwide Need a Checkup
Authors: Derouin Sarah
Published: April 2024

Lakes are facing a slew of health issues that may become chronic. Can human health care strategies help?
Side-effects of mdma-assisted psychotherapy: a systematic review and meta-analysis
Authors: Colcott Julia , Guerin Alexandre A. , Carter Olivia , Meikle Sally , Bedi Gillinder
Published: April 2024

Evidence suggests that MDMA-assisted psychotherapy (MDMA-AP) has therapeutic potential for treatment of psychiatric illness. We conducted the first comprehensive systematic review and meta-analysis of the side effects of MDMA-AP across indications. We also assessed the quality of side effects-reporting in published trials of MDMA-AP. PubMed, EMBASE, PsycINFO, MEDLINE and Cochrane Central Register of Controlled Trials (CENTRAL) were systematically searched. Phase 2 and 3 MDMA-AP studies were included; Phase 1 studies, which assessed MDMA without psychotherapy, were not. Quality of side effects-reporting was assessed against the CONSORT Harms 2022 guidelines. We also compared numbers of adverse events reported in publications to those recorded in ClinicalTrial.gov registers. Thirteen studies were included, with eight contributing to the meta-analysis. In Phase 2 studies, MDMA-AP was associated with increased odds of any side effect during medication sessions (OR = 1.67, 95%CI (1.12, 2.49)) and in the 7 days following (OR = 1.59, 95%CI (1.12, 2.24)) relative to control conditions. In Phase 3 studies, MDMA-AP was associated with increased odds of any adverse event during the treatment period relative to placebo-assisted psychotherapy (OR = 3.51, 95%CI (2.76, 4.46)). The majority of RCTs were rated as having high risk of bias. Certainty of the evidence was rated as very low to moderate according to the GRADE framework. No included RCT had adequate adherence to the CONSORT Harms 2022 recommendations and reporting rates were also low. Compared to placebo, MDMA-AP was associated with increased odds of side effects, which were largely transient and mild or moderate in severity. However, identified limitations in existing evidence indicate that further investigation is needed to better characterize the safety profile of MDMA-AP and guide implementation.
Fear of childbirth and its associated factors among pregnant women in Dejen Woreda, East Gojjam Zone, Northwest Ethiopia: a community-based cross-sectional study
Authors: Alemu Chekol , Wudu Habitamu , Lakew Samuel
Published: April 2024

Fears of delivery are the uncertainty and worry experienced before, during, and following labor. It hurts women's health and affects 5–40% of all moms globally. If not recognized, it could cause expectant mothers to feel alone and unsupported. Studies on this subject, however, are scarce at the woreda level. Therefore, this study amis to assess the prevalence and associated factors of fear of childbirth among pregnant women in Dejen Woreda, East Gojjam Zone, Northwest Ethiopia. A community-based cross-sectional study was conducted among 575 pregnant women selected by Cluster Sapling from December 15 to December 25, 2022. Data were gathered using a structured questionnaire that was presented by an interviewer. Data were entered using Epi-data version 3.1 and analyzed using SPSS Version 23 statistical software. Descriptive statistics and inferential statistics were done, and ordinary logistic regression was used to examine the associated factor for fear of childbirth. Finally, a P-value < 0.05 was used to determine statistical significance. Among the 575 pregnant women supposed to have participated, 560 agreed and participated in the survey, with a response rate of 97.4%. This study showed that 133(23.8%; CI 20.4–26.8) of the study participants had low fear of childbirth, 67(12%; CI (9.3–14.8 moderate, 217 (38.8%; CI 34.6–42.7) high, 143 (25.5%; CI 21.8–29.1) severe fear of childbirth. Having maternal age 18–24 (adjusted odds ratio/AOR = 1.6; 95% CI (1.1–2.3), p-value = -0.08), occupation daily laborer and other (AOR = 0.3,95%; CI 0.3, 0–74; p-value = 0.004),gestational age in third trimester (AOR = 1.9,95%; CI 1.1–3.4), p-value = 0.022) showed significant factor for a fear of childbirth. Maternal age, occupation, and third-trimester pregnancy were found to be significantly associated with fear of childbirth. Women should engage in special attention to keep them healthy by consistent monitoring during pregnancy. Healthcare providers should identify pregnant women with high fear of childbirth early, offer cognitive behavioral therapy, support psychological and physical well-being, provide early age and preventive measures, and use uniform instruments for assessing women's anxiety, promoting systematic reviews and longitudinal studies.
Enhancing physical characteristics and antibacterial efficacy of chitosan through investigation of microwave-assisted chemically formulated chitosan-coated ZnO and chitosan/ZnO physical composite
Authors: Ali Sara A. , Ali E. S. , Hamdy G. , Badawy Mona Shaban E. M. , Ismail Abdallah R. , El-Sabbagh Inas. A. , El-Fass Magda M. , Elsawy Moataz A.
Published: April 2024

This study investigates the creation and analysis of chitosan-zinc oxide (CS-ZnO) nanocomposites, exploring their effectiveness in inhibiting bacteria. Two synthesis approaches, physical and chemical, were utilized. The CS-ZnO nanocomposites demonstrated strong antibacterial properties, especially against Staphylococcus aureus, a Gram-positive bacterium. Chemically synthesized nanocomposites (CZ10 and CZ100) exhibited larger inhibition zones (16.4 mm and 18.7 mm) compared to physically prepared CS-Z5 and CS-Z20 (12.2 mm and 13.8 mm) against Staphylococcus aureus. Moreover, CZ nanocomposites displayed enhanced thermal stability, with decomposition temperatures of 281°C and 290°C, surpassing CS-Z5 and CS-Z20 (260°C and 258°C). The residual mass percentages at 800°C were significantly higher for CZ10 and CZ100 (58% and 61%) than for CS-Z5 and CS-Z20 (36% and 34%). UV–Visible spectroscopy revealed reduced band gaps in the CS-ZnO nanocomposites, indicating improved light absorption. Transmission electron microscopy (TEM) confirmed uniform dispersion of ZnO nanoparticles within the chitosan matrix. In conclusion, this research underscores the impressive antimicrobial potential of CS-ZnO nanocomposites, especially against Gram-positive bacteria, and highlights their enhanced thermal stability. These findings hold promise for diverse applications in industries such as medicine, pharmaceuticals, and materials science, contributing to the development of sustainable materials with robust antimicrobial properties.
Rainfall’s impact on agricultural production and government poverty reduction efficiency in China
Authors: Wang Jianlin , You Zhanglan , Song Pengfei , Fang Zhong
Published: April 2024

The quest to eradicate poverty, central to the United Nations Sustainable Development Goals (SDGs), poses a significant global challenge. Advancement in sustainable rural development is critical to this effort, requiring the seamless integration of environmental, economic, and governmental elements. Previous research often omits the complex interactions among these factors. Addressing this gap, this study evaluates sustainable rural development in China by examining the interconnection between agricultural production and government-led poverty reduction, with annual rainfall considered an influential factor of climate change impacts on these sectors and overall sustainability. Utilizing a Meta-frontier entropy network dynamic Directional Distance Function (DDF) within an exogenous Data Envelopment Analysis (DEA) model, we categorize China’s 27 provinces into southern and northern regions according to the Qinling-Huaihe line for a comparative study of environmental, economic, and governmental efficiency. This innovative approach overcomes the limitations of previous static analyses. The findings reveal: (1) Rainfall, as an exogenous variable, significantly affects agricultural production efficiency. (2) The overall efficiency in both southern and northern regions increases when accounting for rainfall. (3) Government effectiveness in poverty reduction is comparatively lower in the northern region than in the southern region when rainfall is considered. These insights underscore the importance of including climatic variables in sustainable development policies and emphasize the need for region-specific strategies to bolster resilience against climatic challenges.
Biodiversity of carbapenem-resistant bacteria in clinical samples from the Southwest Amazon region (Rondônia/Brazil)
Authors: dos Santos Levy Assis , Cayô Rodrigo , Valiatti Tiago Barcelos , Gales Ana Cristina , de Araújo Larissa Fatarelli Bento , Rodrigues Fernando Marques , de Carvalho Tatiane Silva , Vaz Marcos André Braz , Campanharo Marcela
Published: April 2024

Brazil is recognized for its biodiversity and the genetic variability of its organisms. This genetic variability becomes even more valuable when it is properly documented and accessible. Understanding bacterial diversity through molecular characterization is necessary as it can improve patient treatment, reduce the length of hospital stays and the selection of resistant bacteria, and generate data for health and epidemiological surveillance. In this sense, in this study, we aimed to understand the biodiversity and molecular epidemiology of carbapenem-resistant bacteria in clinical samples recovered in the state of Rondônia, located in the Southwest Amazon region. Retrospective data from the Central Public Health Laboratories (LACEN/RO) between 2018 and 2021 were analysed using the Laboratory Environment Manager Platform (GAL). Seventy-two species with carbapenem resistance profiles were identified, of which 25 species carried at least one gene encoding carbapenemases of classes A (blaKPC-like), B (blaNDM-like, blaSPM-like or blaVIM-like) and D (blaOXA-23-like, blaOXA-24-like, blaOXA-48-like, blaOXA-58-like or blaOXA-143-like), among which we will highlight Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Serratia marcescens, and Providencia spp. With these results, we hope to contribute to the field by providing epidemiological molecular data for state surveillance on bacterial resistance and assisting in public policy decision-making.
Behavioral difficulties and associated factors among the ‘lost generation’ of Syrian children and adolescents
Authors: Alsharif Aya , Al Habbal Osama , Gabadian Aram , El Maamoun Riwa , Al Faraj Alaa , kamr aldin Taima , Haitham Aldammad Ola , Alkayakhi Ahmad , Al Habbal Aya
Published: April 2024

Childhood and adolescence, vital in shaping adult life and society, are profoundly impacted during conflicts like Syria’s devastating war. This study explores the prevalence of behavioral disorders in Syrian children and adolescents, examining the influence of war and family-related factors. This cross-sectional study was conducted on children aged 2–17 years at a children’s outpatient clinic in Damascus, Syria. We assessed parents’ quality of life, war and family-related factors, and behavioral difficulties through parental interviews using two questionnaires: the Arabic version of the Strengths & Difficulties Questionnaire (SDQ) and the brief Arabic version of the World Health Organization Quality of Life Questionnaire (WHOQOL-BREF). 74.67% of children aged 2–4 years and 61.29% of children aged 5–17 years were experiencing behavioral difficulties, with emotional difficulties being the most prevalent ones. Children exposed to kidnapping, family losses, lack of school enrollment, and those with parents having lower education, lower socioeconomic status, and poorer quality of life exhibited higher Total SDQ scores. The high prevalence of behavioral difficulties among children and adolescents in Syria is a major concern, with both direct and indirect war-related factors contributing to this issue.
Évolution de la résistance au cancer dans le monde animal
Authors: Thomas Frédéric , Ujvari Beata , Dujon Antoine M.
Published: April 2024

Le cancer est un dommage collatéral inévitable inhérent à l’évolution des organismes multicellulaires, apparus à la fin du Précambrien. L’exploration de la manière dont les animaux, en particulier ceux de grande taille et de longue durée de vie, font face au cancer, comporte des enjeux à la fois fondamentaux et appliqués. Dans cet article, nous commençons par présenter le cadre conceptuel nécessaire pour comprendre les théories qui traitent de l’évolution des défenses anti-cancéreuses. Nous présentons ensuite un certain nombre d’exemples, notamment les rats-taupes nus, les éléphants, les baleines, les xénarthres (paresseux, tatous et fourmiliers), les chauves-souris et les placozoaires1. Les contributions de la génomique comparative à la compréhension des convergences évolutives sont également abordées. Enfin, nous indiquons que la sélection naturelle a également favorisé des adaptations visant à éviter les zones mutagènes, par exemple, ou à maximiser l’effort de reproduction immédiat en cas de cancer. L’exploration de ces solutions, intéressante conceptuellement, pourrait aussi permettre d’envisager de nouvelles approches thérapeutiques pour la santé humaine.
Organ-delimited gene regulatory networks provide high accuracy in candidate transcription factor selection across diverse processes
Authors: Ranjan Rajeev , Srijan Sonali , Balekuttira Somaiah , Agarwal Tina , Ramey Melissa , Dobbins Madison , Kuhn Rachel , Wang Xiaojin , Hudson Karen , Li Ying , Varala Kranthi
Published: April 2024

Organ-specific gene expression datasets that include hundreds to thousands of experiments allow the reconstruction of organ-level gene regulatory networks (GRNs). However, creating such datasets is greatly hampered by the requirements of extensive and tedious manual curation. Here, we trained a supervised classification model that can accurately classify the organ-of-origin for a plant transcriptome. This K-Nearest Neighbor-based multiclass classifier was used to create organ-specific gene expression datasets for the leaf, root, shoot, flower, and seed in
Arabidopsis thaliana
. A GRN inference approach was used to determine the: i. influential transcription factors (TFs) in each organ and, ii. most influential TFs for specific biological processes in that organ. These genome-wide, organ-delimited GRNs (OD-GRNs), recalled many known regulators of organ development and processes operating in those organs. Importantly, many previously unknown TF regulators were uncovered as potential regulators of these processes. As a proof-of-concept, we focused on experimentally validating the predicted TF regulators of lipid biosynthesis in seeds, an important food and biofuel trait. Of the top 20 predicted TFs, eight are known regulators of seed oil content, e.g., WRI1, LEC1, FUS3. Importantly, we validated our prediction of MybS2, TGA4, SPL12, AGL18, and DiV2 as regulators of seed lipid biosynthesis. We elucidated the molecular mechanism of MybS2 and show that it induces purple acid phosphatase family genes and lipid synthesis genes to enhance seed lipid content. This general approach has the potential to be extended to any species with sufficiently large gene expression datasets to find unique regulators of any trait-of-interest.
Sustainable and Healthy Gastronomy in Costa Rica – Betting on Sustainable Diets
Authors: Echandi Marcela Dumani , Masis Patricia Sedó , Viquez Randall García , Rodriguez Roberto Azofeifa
Published: April 2024

Current consumption patterns in Costa Rica, and other countries, are often driven by misinformation and lack of knowledge about the nutritional value and sustainability of food products, which leads to unhealthy diets and loss of food biodiversity. In 2012, Costa Rica launched a multi-stakeholder initiative, The National Plan on Healthy and Sustainable Gastronomy (hereafter referred to as the Plan), to reverse this trend. Healthy and Sustainable Gastronomy (HSG) is an innovative paradigm for the sustainability of natural resources based on the consumers’ decision to prepare and enjoy food to be healthy. It is one of various approaches and solutions provided by One Planet Network, which hosts the UN Sustainable Food Systems Programme. Costa Rica’s Plan promotes a new healthy and sustainable national cuisine as a driving force to create a sustainable food system and improve the health of the population through diet. The cuisine is designed to increase the use of local, traditional and plant-based foods and aims to reduce obesity and micronutrient deficiencies. Additional benefits are food security, increased food biodiversity and tourism through gastronomic food culture. This case study explains the concept of HSG and how Costa Rica implemented it to change consumer behaviour; support local producers; achieve national goals for sustainable development; promote tourism and improve population health. Important progress has been made, but lack of information and awareness about linkages connecting food production and consumption is a major barrier. Information challenges include a lack of traceability systems and eco-labelling to build consumer trust, ongoing consumer education, and ensuring production will satisfy demand. Overall, the authors believe that Costa Rica will act as a model of HSG for other countries to follow in order to achieve sustainable food production and consumption, bridging the gap between emerging markets and Western economies.

Information

© CAB International 2019
Influence of heavy Canadian crude oil on pristine freshwater boreal lake ecosystems in an experimental oil spill
Authors: Kharey Gurpreet S , Palace Vince , Whyte Lyle , Greer Charles W
Published: April 2024

The overall impact of a crude oil spill into a pristine freshwater environment in Canada is largely unknown. To evaluate the impact on the native microbial community, a large-scale in situ model experimental spill was conducted to assess the potential role of the natural community to attenuate hydrocarbons. A small volume of conventional heavy crude oil (CHV) was introduced within contained mesocosm enclosures deployed on the shoreline of a freshwater lake. The oil was left to interact with the shoreline for 72 h and then free-floating oil was recovered using common oil spill response methods (i.e. freshwater flushing and capture on oleophilic absorptive media). Residual polycyclic aromatic hydrocarbon (PAH) concentrations returned to near preoiling concentrations within 2 months, while the microbial community composition across the water, soil, and sediment matrices of the enclosed oligotrophic freshwater ecosystems did not shift significantly over this period. Metagenomic analysis revealed key polycyclic aromatic and alkane degradation mechanisms also did not change in their relative abundance over the monitoring period. These trends suggest that for small spills (<2 l of oil per 15 m2 of surface freshwater), physical oil recovery reduces polycyclic aromatic hydrocarbon concentrations to levels tolerated by the native microbial community. Additionally, the native microbial community present in the monitored pristine freshwater ecosystem possesses the appropriate hydrocarbon degradation mechanisms without prior challenge by hydrocarbon substrates. This study corroborated trends found previously (Kharey et al. 2024) toward freshwater hydrocarbon degradation in an environmentally relevant scale and conditions on the tolerance of residual hydrocarbons in situ.
The role of ion homeostasis in adaptation and tolerance to acetic acid stress in yeasts
Authors: Antunes Miguel , Sá-Correia Isabel
Published: April 2024

Maintenance of asymmetric ion concentrations across cellular membranes is crucial for proper yeast cellular function. Disruptions of these ionic gradients can significantly impact membrane electrochemical potential and the balance of other ions, particularly under stressful conditions such as exposure to acetic acid. This weak acid, ubiquitous to both yeast metabolism and industrial processes, is a major inhibitor of yeast cell growth in industrial settings and a key determinant of host colonization by pathogenic yeast. Acetic acid toxicity depends on medium composition, especially on the pH (H+ concentration), but also on other ions’ concentrations. Regulation of ion fluxes is essential for effective yeast response and adaptation to acetic acid stress. However, the intricate interplay among ion balancing systems and stress response mechanisms still presents significant knowledge gaps. This review offers a comprehensive overview of the mechanisms governing ion homeostasis, including H+, K+, Zn2+, Fe2+/3+, and acetate, in the context of acetic acid toxicity, adaptation, and tolerance. While focus is given on Saccharomyces cerevisiae due to its extensive physiological characterization, insights are also provided for biotechnologically and clinically relevant yeast species whenever available.
Human costal cartilage, tooth cavities, and femur nutrient canals—new niches for insects used in forensic entomology
Authors: Tomsia Marcin , Grzywacz Andrzej , Szpila Krzysztof , Walczak Kinga , Mahlerová Karolina , Vaněk Daniel , Matuszewski Szymon
Published: April 2024

The study aimed to analyze the entomological material collected during 13 autopsies performed on the unidentified cadavers revealed at different stages of decay in the Upper Silesia Region (Poland) over 2016–2022. During the preparation of human tissues for genetic identification, we revealed larvae, puparia, and adult insects in previously undescribed locations: costal cartilage, femur nutrient canals (foramen nutrients), and tooth cavities. The taxonomical assessment was done using morphological examination or DNA barcoding, where necessary. Based on our observations, we conclude that the apical constriction, foramen, and cavities may serve as migration paths inside teeth, and the femur nutrient canals to the bone marrow. The study also revealed that Necrobia ruficollis (Fabricius, 1775) and Pyralidae Latreille, 1802 (Phycitinae) moths can form pupal chambers inside the costal cartilage, indicating that these insects can complete their life cycle inside this cache. We believe that the newly reported locations of carrion insects in human remains may be relevant to forensic entomology, as they provide new opportunities to collect insect evidence.
New citizen science initiative enhances flowering onset predictions for fruit trees in Great Britain
Authors: Wyver Chris , Potts Simon G , Pitts Richard , Riley Mike , Janetzko Gerard , Senapathi Deepa
Published: April 2024

Accurately predicting flowering phenology in fruit tree orchards is crucial for timely pest and pathogen treatments and for the introduction of managed pollinators. Making predictions requires large datasets of flowering dates, which are often limited to single locations. Consequently, resulting phenology predictions are not representative across larger geographic areas. Citizen science may offer a solution to this data gap, with millions of biological records across a wide range of taxa recorded annually. Here, a new citizen science platform called “FruitWatch” is introduced, monitoring flowering dates of fruit trees in Great Britain. The objectives of this study are to assess the suitability of FruitWatch submissions to 1) detect latitudinal variation in flowering onset dates, 2) parameterize existing phenology modelling frameworks, and 3) make predictions of flowering onset dates across Great Britain for a single year.

Using data for four cultivars from 2022, linear models reveal significant latitudinal delays in flowering onset of as much as 1.49±0.63 days per degree latitude further north (Pear ‘Conference’), with significant delays also seen in Cherry ‘Stella’ (1.39±0.48 days) and Plum ‘Victoria’ (1.22±0.18 days). FruitWatch informed phenology modelling frameworks performed well for predicting flowering onset, with root-mean-square Error values of predictions from validation datasets ranging between 4.6 (‘Victoria’) and 8.0 (‘Conference’) days. The parameterized models also provided realistic flowering onset predictions across Great Britain in 2022, with earlier flowering dates predicted in warmer areas. These findings demonstrate the potential of citizen science data to offer growers cultivar- and location-specific phenology predictions to help inform orchard management.
Differential analysis of immunoglobulin gene expression pattern in chickens of distinct breeds and developmental periods
Authors: Qiu Yanbo , Yi Xiaohua , Tang Xiaoqin , Wei Yanpei , Zhang Beibei , Duan Shunan , Wang Shuhui , Sun Xiuzhu
Published: April 2024

Immunoglobulin is an essential component of the body’s defense against pathogens, aiding in the recognition and clearance of foreign antigens. Research concerning immunoglobulin gene and its diversity of expression across different breeds within the same species is relatively scarce. In this study, we employed RACE (Rapid Amplification of cDNA Ends) technology, prepared DNA libraries, performed high-throughput sequencing, and conducted related bioinformatics analysis to analyze the differences in immunoglobulin gene diversity and expression at different periods in Hy-line brown hens, Lueyang black-bone chickens, and Beijing-You chickens. The study found that the composition of chicken immunoglobulin genes is relatively simple, with both the light chain and heavy chain having a functional V gene. Additionally, the mechanisms of immunoglobulin diversity generation tended to be consistent among different breeds and periods of chickens, primarily relying on abundant junctional diversity, somatic hypermutation (SHM), and gene conversion (GCV) to compensate for the limitations of low-level V(D)J recombination. As the age increased, the junctional diversity of IgH and IgL tended to diversify and showed similar expression patterns among different breeds. In the three chicken breeds, the predominant types of mutations observed in IGHV and IGLV SHM were A to G and G to A transitions. Specifically, IGLV exhibited a preference for A to G mutations, whereas IGHV displayed a bias toward G to A mutations. The regions at the junctions between framework regions (FR) and complementarity-determining regions (CDR) and within the CDR regions themselves are typically prone to mutations. The locations of GCV events in IGLV and IGHV do not show significant differences, and replacement segments are concentrated in the central regions of FR1, CDR, and FR2. Importantly, gene conversion events are not random occurrences. Additionally, our investigation revealed that CDRH3 in chickens of diverse breeds and periods the potential for diversification through the incorporation of cysteine. This study demonstrates that the diversity of immunoglobulin expression tends to converge among Hy-line brown hens, Lueyang black-bone chickens, and Beijing-You chickens, indicating that the immunoglobulin gene expression mechanisms in different breeds of chickens do not exhibit significant differences due to selective breeding.