bluuebunny/crossref_metadata_2025 huggingface dataset:
Changing mechanical properties of photopolymerized, dityrosine-crosslinked protein-based hydrogels
Authors: Haas Sandra , Körner Saskia , Zintel Laura , Hubbuch Jürgen
Published: September 2022

Hydrogels based on renewable resources are a promising class of materials for future applications in pharmaceutics, drug delivery and personalized medicine. Thus, optional adjustments of mechanical properties such as swelling behavior, elasticity and network strength are desired. In this context, hydrogels based on the biological raw materials bovine serum albumin and casein were prepared by dityrosine-crosslinking of their tyrosine residues through visible light-induced photopolymerization. Changing the tyrosine accessibility by urea addition before photopolymerization increased the storage modulus of the hydrogels by 650% while simultaneously being more elastic. Furthermore, contributions of the buffer system composition, variation of protein concentration and storage medium towards mechanical properties of the hydrogel such as storage moduli, elasticity, fracture strain, compressive strength and relative weight swelling ratio are discussed. It could be shown, that changes in precursor solution and storage medium characteristics are crucial parameters towards tuning the mechanical properties of protein-based hydrogels.
Mapping the functional anatomy and topography of the cardiac autonomic innervation for selective cardiac neuromodulation using MicroCT
Authors: Kronsteiner Bettina , Zopf Lydia M. , Heimel Patrick , Oberoi Gunpreet , Kramer Anne M. , Slezak Paul , Weninger Wolfgang J. , Podesser Bruno K. , Kiss Attila , Moscato Francesco
Published: September 2022

Background: Vagus nerve stimulation (VNS) has gained great importance as a promising therapy for a myriad of diseases. Of particular interest is the therapy of cardiovascular diseases, such as heart failure or atrial fibrillation using selective cardiac VNS. However, there is still a lack of organ-specific anatomical knowledge about the fascicular anatomy and topography of the cardiac branch (CB), which diminishes the therapeutic possibilities for selective cardiac neuromodulation. Here, we established a topographical and anatomical map of the superior cardiac VN in two animal species to dissect cervical and cardiac VN morphology.

Methods: Autonomic nerves including superior CBs were harvested from domestic pigs and New Zeeland rabbits followed by imaging with microcomputed tomography (µCT) and 3D rendering. The data were analyzed in terms of relevant topographical and anatomical parameters.

Results: Our data showed that cardiac vagal fascicles remained separated from other VN fascicles up to 22.19 mm (IQR 14.02–41.30 mm) in pigs and 7.68 mm (IQR 4.06–12.77 mm) in rabbits from the CB point and then started merging with other fascicles. Exchanges of nerve fascicles between sympathetic trunk (ST) and VN were observed in 3 out of 11 nerves, which might cause additional unwanted effects in unselective VNS. Our 3D rendered digital model of the cardiac fascicles was generated showing that CB first remained on the medial side where it branched off the VN, as also shown in the µCT data of 11 pig nerves, and then migrated towards the ventromedial site the further it was traced cranially.

Conclusion: Our data provided an anatomical map of the cardiac vagal branches including cervical VN and ST for future approaches of selective cardiac neurostimulation, indicating the best position of selective cardiac VNS just above the CB point.
Direct preparation of solid carbon dots by pyrolysis of collagen waste and their applications in fluorescent sensing and imaging
Authors: Qin Xiaoyun , Fu Cuicui , Zhang Jin , Shao Wenlong , Qin Xiaomei , Gui Yanghai , Wang Lan , Guo Huishi , Chen Fenghua , Jiang Liying , Wu Gang , Bikker Floris J. , Luo Dan
Published: September 2022

The fluorescent carbon dots (CDs) have found their extensive applications in sensing, bioimaging, and photoelectronic devices. In general terms, the synthesis of CDs is straight-forward, though their subsequent purification can be laborious. Therefore, there is a need for easier ways to generate solid CDs with a high conversion yield. Herein, we used collagen waste as a carbon source in producing solid CDs through a calcination procedure without additional chemical decomposition treatment of the raw material. Considering a mass of acid has destroyed the original protein macromolecules into the assembled structure with amino acids and peptide chains in the commercial extraction procedure of collagen product. The residual tissues were assembled with weak intermolecular interactions, which would easily undergo dehydration, polymerization, and carbonization during the heat treatment to produce solid CDs directly. The calcination parameters were surveyed to give the highest conversion yield at 78%, which occurred at 300°C for 2 h. N and S atomic doping CDs (N-CDs and S-CDs) were synthesized at a similar process except for immersion of the collagen waste in sulfuric acid or nitric acid in advance. Further experiments suggested the prepared CDs can serve as an excellent sensor platform for Fe3+ in an acid medium with high anti-interference. The cytotoxicity assays confirmed the biosafety and biocompatibility of the CDs, suggesting potential applications in bioimaging. This work provides a new avenue for preparing solid CDs with high conversion yield.
Homogeneously niobium-doped MoS2 for rapid and high-sensitive detection of typical chemical warfare agents
Authors: Jiang Huaning , Wang Huaizhang , Shangguan Yanlei , Chen Jingfei , Liang Ting
Published: September 2022

Rapid detection of Chemical Warfare Agents (CWAs) is of great significance in protecting civilians in public places and military personnel on the battlefield. Two-dimensional (2D) molybdenum disulfide (MoS2) nanosheets (NSs) can be integrated as a gas sensor at room temperature (25°C) due to their large specific surface area and excellent semiconductor properties. However, low sensitivity and long response-recovery time hinder the pure MoS2 application in CWAs gas sensors. In this work, we developed a CWAs sensor based on in-situ niobium-doped MoS2 NSs (Nb-MoS2 NSs) via direct chemical-vapor-deposition (CVD) growth. Characterization results show that the high content of Nb elements (7.8 at%) are homogeneously dispersed on the large-area 2D structure of MoS2. The Nb-MoS2 NSs-based CWAs sensor exhibits higher sensitivity (−2.09% and −3.95% to 0.05 mg/m3 sarin and sulfur mustard, respectively) and faster response speed (78 s and 30 s to 0.05 mg/m3 sarin and sulfur mustard, respectively) than MoS2 and other 2D materials at room temperature. And the sensor has certain specificity for sarin and sulfur mustard and is especially sensitive to sulfur mustard. This can be attributed to the improvement of adsorption properties via electronic regulation of Nb doping. This is the first report about CWAs detection based on two-dimensional (2D) transition metal dichalcogenides (TMDs) sensing materials, which demonstrates that the high sensitivity, rapid response, and low limit of detection of 2D TMDs-based CWAs sensor can meet the monitoring needs of many scenarios, thus showing a strong application potential.
In vitro and in vivo anti−Toxoplasma activities of HDAC inhibitor Panobinostat on experimental acute ocular toxoplasmosis
Authors: Zhang Yu , Zhang Qingqing , Li Haiming , Cong Hua , Qu Yi
Published: September 2022

Ocular toxoplasmosis (OT) is retinochoroiditis caused by Toxoplasma gondii infection, which poses a huge threat to vision. However, most traditional oral drugs for this disease have multiple side effects and have difficulty crossing the blood-retinal barrier, so the new alternative strategy is required to be developed urgently. Histone deacetylases (HDAC) inhibitors, initially applied to cancer, have attracted considerable attention as potential anti-Toxoplasma gondii drugs. Here, the efficacy of a novel HDAC inhibitor, Panobinostat (LBH589), against T. gondii has been investigated. In vitro, LBH589 inhibited the proliferation and activity of T. gondii in a dose-dependent manner with low toxicity to retinal pigment epithelial (RPE) cells. In vivo, optical coherence tomography (OCT) examination and histopathological studies showed that the inflammatory cell infiltration and the damage to retinal architecture were drastically reduced in C57BL/6 mice upon treatment with intravitreal injection of LBH589. Furthermore, we have found the mRNA expression levels of inflammatory cytokines were significantly decreased in LBH589–treated group. Collectively, our study demonstrates that LBH589 holds great promise as a preclinical candidate for control and cure of ocular toxoplasmosis.
The interactions between traditional Chinese medicine and gut microbiota: Global research status and trends
Authors: Yang Shanshan , Hao Shaodong , Wang Qin , Lou Yanni , Jia Liqun , Chen Dongmei
Published: September 2022

Background

There is a crosstalk between traditional Chinese medicine (TCM) and gut microbiota (GM), many articles have studied and discussed the relationship between the two. The purpose of this study is to use bibliometric analysis to explore the research status and development trends of the TCM/GM research, identify and analyze the highly cited papers relating to the TCM/GM.

Methods

A literature search regarding TCM/GM publications from 2004 to 2021 was undertaken on August 13, 2022. The main information (full record and cited references) of publications was extracted from the Science Citation Index Expanded (SCI-E) of Web of Science Core Collection (WoSCC). The Bibliometrix of R package, CiteSpace and VOSviewer were used for bibliometric analysis.

Results

A total of 830 papers were included. The publication years of papers were from 2004 to 2021. The number of papers had increased rapidly since 2018. China had the most publications and made most contributions to this field. Nanjing University of Chinese Medicine and Beijing University of Chinese Medicine were in the leading productive position in TCM/GM research, Chinese Academy of Chinese Medical Sciences had the highest total citations (TC). Duan Jin-ao from Nanjing University of Chinese Medicine had the largest number of publications, and Tong Xiao-lin from China Academy of Chinese Medical Sciences had the most TC. The Journal of Ethnopharmacology had the most published papers and the most TC. The main themes in TCM/GM included the role of GM in TCM treatment of glucolipid metabolism diseases and lower gastrointestinal diseases; the mechanism of interactions between GM and TCM to treat diseases; the links between TCM/GM and metabolism; and the relationship between GM and oral bioavailability of TCM.

Conclusion

This study gained insight into the research status, hotspots and trends of global TCM/GM research, identified the most cited articles in TCM/GM and analyzed their characteristics, which may inform clinical researchers and practitioners’ future directions.
Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis
Authors: Man Wanrong , Song Xinglong , Xiong Zhenyu , Gu Jing , Lin Jie , Gu Xiaoming , Yu Duan , Li Congye , Jiang Mengyuan , Zhang Xuebin , Yang Zhi , Cao Yang , Zhang Yan , Shu Xiaofei , Wu Dexi , Wang Haichang , Ji Gang , Sun Dongdong
Published: September 2022

As a vital adipokine, Adipsin is closely associated with cardiovascular risks. Nevertheless, its role in the onset and development of cardiovascular diseases remains elusive. This study was designed to examine the effect of Adipsin on survival, cardiac dysfunction and adverse remodeling in the face of myocardial infarction (MI) injury. In vitro experiments were conducted to evaluate the effects of Adipsin on cardiomyocyte function in the face of hypoxic challenge and the mechanisms involved. Our results showed that Adipsin dramatically altered expression of proteins associated with iron metabolism and ferroptosis. In vivo results demonstrated that Adipsin upregulated levels of Ferritin Heavy Chain (FTH) while downregulating that of Transferrin Receptor (TFRC) in peri-infarct regions 1 month following MI. Adipsin also relieved post-MI-associated lipid oxidative stress as evidenced by decreased expression of COX2 and increased GPX4 level. Co-immunoprecipitation and immunofluorescence imaging prove a direct interaction between Adipsin and IRP2. As expected, cardioprotection provided by Adipsin depends on the key molecule of IRP2. These findings revealed that Adipsin could be efficiently delivered to the heart by exosomes derived from pericardial adipose tissues. In addition, Adipsin interacted with IRP2 to protect cardiomyocytes against ferroptosis and maintain iron homeostasis. Therefore, Adipsin-overexpressed exosomes derived from pericardial adipose tissues may be a promising therapeutic strategy to prevent adverse cardiac remodeling following ischemic heart injury.
Comparison of long-term cardiovascular and renal outcomes between percutaneous coronary intervention and coronary artery bypass grafting in multi-vessel disease with chronic kidney disease
Authors: Kwon Woochan , Choi Ki Hong , Jeong Dong Seop , Lee Sang Yoon , Lee Joo Myung , Park Taek Kyu , Yang Jeong Hoon , Hahn Joo-Yong , Choi Seung-Hyuk , Chung Su Ryeun , Cho Yang Hyun , Sung Kiick , Kim Wook Sung , Gwon Hyeon-Cheol , Lee Young Tak , Song Young Bin
Published: September 2022

Objective

This study aims to analyze cardiac and renal outcomes of chronic kidney disease (CKD) patients with multi-vessel disease who have undergone coronary artery bypass grafting (CABG) or percutaneous coronary intervention (PCI).

Materials and methods

Chronic kidney disease patients with multi-vessel disease who underwent CABG or PCI were retrospectively selected from our database and divided into the PCI group [further stratified into PCI with complete revascularization (PCI-CR) and PCI with incomplete revascularization (PCI-IR) groups] and the CABG group. The primary endpoint was the composite of all-cause death, myocardial infarction (MI), or stroke at 5 years. The key secondary endpoint was the 5-year rate of the renal composite outcome, defined as >40% glomerular filtration rate decrease, initiation of dialysis, and/or kidney transplant. Outcomes were compared using Cox proportional hazards regression analysis, and the results were further adjusted by multivariable analyses and inverse probability weighting.

Results

Among the study population (n = 798), 443 (55.5%) patients received CABG and 355 (44.5%) patients received PCI. Compared with the CABG group, the PCI group had similar risk of the primary endpoint (CABG vs. PCI, 19.3% vs. 24.0%, HR: 1.28, 95% CI: 0.95–1.73, p = 0.11) and a lower risk of the renal composite outcome (36.6% vs. 31.2%, HR: 0.74, 95% CI 0.58–0.94, p = 0.03). In addition, PCI-IR was associated with a significantly higher risk of the primary endpoint than CABG (HR: 1.54, 95% CI: 1.11–2.13, p = 0.009) or PCI-CR (HR: 1.78, 95% CI: 1.09–2.89, p = 0.02). However, PCI-CR had a comparable 5-year death, MI, or stroke rate to CABG (HR: 0.86, 95% CI 0.54–1.38, p = 0.54).

Conclusion

Coronary artery bypass grafting showed an incidence of death, MI, or stroke similar to PCI but was associated with a higher risk of renal injury. PCI-CR had a prognosis comparable with that of CABG, while PCI-IR had worse prognosis. If PCI is chosen for revascularization in patients with CKD, achieving CR should be attempted to ensure favorable outcomes.

Clinical trial registration

[clinicaltrials.gov], identifier [NCT 03870815].
High-normal liver enzyme levels in early pregnancy predispose the risk of gestational hypertension and preeclampsia: A prospective cohort study
Authors: Zhang Yi , Sheng Chen , Wang Dingmei , Chen Xiaotian , Jiang Yuan , Dou Yalan , Wang Yin , Li Mengru , Chen Hongyan , He Wennan , Yan Weili , Huang Guoying
Published: September 2022

Background

Gestational hypertension (GH) and preeclampsia (PE) are severe adverse gestational complications. Previous studies supported potential link between elevated liver enzyme levels and GH and PE. However, given the transient physiological reduction of liver enzyme levels in pregnancy, little is known whether the associations of the high-normal liver enzyme levels in early pregnancy with GH and PE exist in pregnant women.

Methods

Pregnant women in this study came from a sub-cohort of Shanghai Preconception Cohort, who were with four liver enzyme levels examined at 9–13 gestational weeks and without established liver diseases, hypertension and preeclampsia. After exclusion of pregnant women with clinically-abnormal liver enzyme levels in the current pregnancy, associations of liver enzyme levels, including alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transpeptidase (GGT), with GH and PE status were assessed by multivariable log-binomial regression. Population attributable fraction was measured to estimate the fractions of GH and PE that were attributable to the high-normal liver enzyme levels.

Results

Among 5,685 pregnant women 160 (2.8%) and 244 (4.3%) developed GH and PE, respectively. After adjustment for potential covariates, higher ALP, ALT and GGT levels were significantly associated with the risk of GH (adjusted risk ratio (aRR):1.21 [95% confidence interval, 1.05–1.38]; 1.21 [1.05–1.38]; and 1.23 [1.09–1.39]), as well as the risk of PE(1.21 [1.13–1.29]; 1.15 [1.03–1.28]; 1.28 [1.16–1.41]), respectively. The cumulative population attributable fraction of carrying one or more high-normal liver enzyme levels (at 80th percentile or over) was 31.4% for GH and 23.2% for PE, respectively.

Conclusion

Higher ALT, ALP and GGT levels within the normal range in early pregnancy are associated with increased risk of GH and PE. The documented associations provide new insight to the role of hepatobiliary function in GH and PE pathogenesis.
Characterization of the sex-specific pattern of angiogenesis and lymphangiogenesis in aortic stenosis
Authors: Matilla Lara , Martín-Núñez Ernesto , Garaikoetxea Mattie , Navarro Adela , Vico Julieta Anabela , Arrieta Vanessa , García-Peña Amaia , Fernández-Celis Amaya , Gainza Alicia , Álvarez Virginia , Sádaba Rafael , López-Andrés Natalia , Jover Eva
Published: September 2022

Objective

We aim to analyze sex-related differences in angiogenesis and lymphangiogenesis in aortic valves (AVs) and valve interstitial cells (VICs) from aortic stenosis (AS) patients.

Approach and Results

Totally 230 patients (59% men) with severe AS undergoing surgical valve replacement were recruited. The density of total neovessels was higher in AVs from men as compared to women. Both small and medium neovessels were more abundant in men's AVs. Accordingly, male AVs exhibited higher CD31 and VE-cadherin expressions. The levels of the pro-angiogenic markers, such as vascular endothelial growth factor (VEGF)-A, VEGF receptor (VEGFR)1, VEGFR2, insulin-like growth factor-binding protein-2 (IGFBP-2), interleukin (IL)-8, chemerin, and fibroblast growth factor (FGF)-7, were increased in AVs from men. Transforming growth factor-β expression was higher in male AVs. The expression of antiangiogenic molecules thrombospondin (Tsp)-1, endostatin, and CD36 was upregulated in male AVs, although the levels of Tsp-2, IL-4, IL-12p70, and chondromodulin-1 were similar between both sexes. The number of lymphatic vessels and the expression of the lymphangiogenic markers Lyve-1 and D2-40 was higher in men's AV as well as VEGF-C, VEGF-D, and VEGFR3. Multivariate analyses adjusted for confounders further validated the sex-dependent expression of these targets. VICs isolated from men's AVs secreted higher amounts of the pro-angiogenic factors, VEGF-A, VEGFR1, IGFBP-2, and FGF-7, as well as the pro-lymphangiogenic factors, VEGF-C, VEGF-D, and VEGFR3, than women without changes in antiangiogenic markers.

Conclusion

Our data show that aberrant angiogenic and lymphangiogenic cues are over-represented in male AVs. Importantly, the VIC is a relevant source of multiple morphogens involved in angiogenesis and lymphangiogenesis likely endowing the AV of men with the predominant calcific AS phenotypes.
Testing the use of continental standardized growth curves (SGCs) for De estimation on coarse quartz grains from Lake Woods, Northern Australia
Authors: Rui Xue , Li Bo , Cohen Tim J.
Published: September 2022

Using continental SGCs built by Fu et al. (2020) can save significant machine time for constructing SGCs when compared to traditional SGC methods, which need significantly more time for building sample-specific or regional SGCs. In this study, the reliability of continental SGCs is tested using coarse quartz grains (180–212 µm) from Lake Woods in Northern Australia. De values obtained by continental SGCs are consistent with De values obtained by regional SGCs and by the SAR procedures.
Pumped storage hydropower in an abandoned open-pit coal mine: Slope stability analysis under different water levels
Authors: Liu Feiyue , Yang Ke , Yang Tianhong , Gao Yuan , Li Jinduo , Liu Qinjie , Fu Qiang
Published: September 2022

Many coal mines are being abandoned for economic and environmental reasons in China. The repurposing of abandoned open-pit coal mines into pumped storage hydropower (PSH) can help with the storage of renewable energy, improve mine environments, and provide added economic value. Construction of PSH plant will change the water level of the abandoned pit, which is envisaged as the lower reservoir, thus influencing the slope stability. In this study, the Fushun West Open-Pit coal mine was taken as an example. Borehole investigation and tilt photogrammetry were used to obtain the rock mass quality and volume of the abandoned pit. A natural lake, dumps, and another open-pit mine are envisaged as the upper reservoir, which could bring 40, 3,200, and 2,000 MW electricity installed capacity, and −295 m, −200 m, and −150 m water levels in the lower reservoir. The numerical analysis software GeoStudio was used for slope stability analysis considering the hydraulic effect under different water levels. A potential landslide risk may happen in several zones under specific water levels. Further imperviousness and reinforcement work is needed to guarantee slope stability for PSH plant construction in the Fushun West Open-Pit coal mine.
Early glucose metabolism in children at risk for type 1 diabetes based on islet autoantibodies compared to low-risk control groups
Authors: Helminen Olli , Pokka Tytti , Aspholm Susanna , Ilonen Jorma , Simell Olli , Knip Mikael , Veijola Riitta
Published: September 2022

Background

Anatomic variation or early differences in glucose metabolism have been linked to the development of type 1 diabetes. We aimed to describe early glucose metabolism based on HbA1c, oral glucose tolerance test (OGTT), and random plasma glucose years before the presentation of type 1 diabetes in five risk groups based on autoantibody combinations. For the first time, we were able to include for comparison children with very low risk of progression to type 1 diabetes.

Methods

The Finnish Diabetes Prediction and Prevention birth cohort study screened newborn infants for HLA susceptibility to type 1 diabetes since 1994. Those carrying a risk genotype were prospectively followed up with islet autoantibody testing. Glucose parameters were obtained starting from the time of seroconversion. By 31 August 2014, 1162 children had developed at least one islet autoantibody and were included in the current study. Type 1 diabetes was diagnosed in 335 children (progressors). In the non-progressor groups, 207 developed multiple (≥2) biochemical islet autoantibodies, 229 a single biochemical autoantibody, 370 ICA only, and 64 transient autoantibodies. Children were divided into five risk groups. Glucose metabolism was evaluated.

Results

We observed lower HbA1c values in early follow-up 4.5 to 6.0 years before diagnosis in the progressors when compared to the same time in children with a single biochemical autoantibody or low-risk (ICA only and transient) participants, who did not progress to clinical type 1 diabetes. However, no such differences were observed in OGTTs or random plasma glucose. The variation was minimal in glucose values in the low-risk groups.

Conclusion

We report the possibility of early alteration in glucose metabolism in future progressors. This could suggest early defects in multiple glucose-regulating hormones.
Multiple tachykinins and their receptors characterized in the gastropod mollusk Pacific abalone: Expression, signaling cascades, and potential role in regulating lipid metabolism
Authors: Lee Seungheon , Kim Mi Ae , Park Jong-Moon , Park Keunwan , Sohn Young Chang
Published: September 2022

Tachykinin (TK) families, including the first neuropeptide substance P, have been intensively explored in bilaterians. Knowledge of signaling of TK receptors (TKRs) has enabled the comprehension of diverse physiological processes. However, TK signaling systems are largely unknown in Lophotrochozoa. This study identified two TK precursors and two TKR isoforms in the Pacific abalone Haliotis discus hannai (Hdh), and characterized Hdh-TK signaling. Hdh-TK peptides harbored protostomian TK-specific FXGXRamide or unique YXGXRamide motifs at the C-termini. A phylogenetic analysis showed that lophotrochozoan TKRs, including Hdh-TKRs, form a monophyletic group distinct from arthropod TKRs and natalisin receptor groups. Although reporter assays demonstrated that all examined Hdh-TK peptides activate intracellular cAMP accumulation and Ca2+ mobilization in Hdh-TKR-expressing mammalian cells, Hdh-TK peptides with N-terminal aromatic residues and C-terminal FXGXRamide motifs were more active than shorter or less aromatic Hdh-TK peptides with a C-terminal YXGXRamide. In addition, we showed that ligand-stimulated Hdh-TKRs mediate ERK1/2 phosphorylation in HEK293 cells and that ERK1/2 phosphorylation is inhibited by PKA and PKC inhibitors. In three-dimensional in silico Hdh-TKR binding modeling, higher docking scores of Hdh-TK peptides were consistent with the lower EC50 values in the reporter assays. The transcripts for Hdh-TK precursors and Hdh-TKR were highly expressed in the neural ganglia, with lower expression levels in peripheral tissues. When abalone were starved for 3 weeks, Hdh-TK1 transcript levels, but not Hdh-TK2, were increased in the cerebral ganglia (CG), intestine, and hepatopancreas, contrasting with the decreased lipid content and transcript levels of sterol regulatory element-binding protein (SREBP). At 24 h post-injection in vivo, the lower dose of Hdh-TK1 mixture increased SREBP transcript levels in the CG and hepatopancreas and accumulative food consumption of abalone. Higher doses of Hdh-TK1 and Hdh-TK2 mixtures decreased the SREBP levels in the CG. When Hdh-TK2-specific siRNA was injected into abalone, intestinal SREBP levels were significantly increased, whereas administration of both Hdh-TK1 and Hdh-TK2 siRNA led to decreased SREBP expression in the CG. Collectively, our results demonstrate the first TK signaling system in gastropod mollusks and suggest a possible role for TK peptides in regulating lipid metabolism in the neural and peripheral tissues of abalone.
A feasibility study on Balarbhita for advancing rural electrification with a solar—Micro-hydro hybrid system
Authors: Kanna R. Rajesh , Singh R. Raja
Published: September 2022

The study analyzed the techno-economic feasibility of a village in the Indian state of Assam and designs a framework for a rural hybrid energy system. Through an extensive resource evaluation, techno-economic feasibility analysis and system size optimization are carried out using HOMER Pro software. Batteries are being used as a form of energy storage in the community power system, which is based on solar PV and micro-hydro as its principal energy sources. Macro-economic variables and resource variations have been considered in sensitivity analysis to ensure the robustness and commercial viability of the proposed hybrid energy systems. A reliable and economical design can provide 279861 kWh of electricity for the village at a modest cost of energy (COE) of $0.0953/kWh using 118 kW of solar power, 11 kW of hydropower, and 261 kWh of battery storage. Furthermore, solar energy systems account for about 62.5 percent, while micro-hydro turbines account for 37.5 percent. In addition, the suggested system does not pollute the local environment caused by carbon emissions. Most importantly, this hybrid energy system is capable of providing 24/7 continuous electricity to the site under consideration.
Review of the input-output network and its application in energy and mineral industries
Authors: Weidong Li , Jianwu Li , Bojie Wen , Mei Han
Published: September 2022

Nowadays, it has become a widespread consensus to deal with global warming through carbon emission reduction among mainstream scientists in the world. As the main battlefield and main force to achieve carbon peak and carbon neutrality, the energy and mineral industries play a crucial role. At the same time, as the basic industries provide energy and raw materials, the energy and mineral industries and other industries form a complex and integrated economic system with each other through input-output correlation. It can provide scientific reference for policymakers and market investors to quantitatively reveal the overall structure of the industry and deeply analyze the role and position of energy and mineral industries in it. Combining the input-output analysis with the complex network theory, the input-output network is a set of theoretical methods with strong theory and application to describe the industrial association structure both between economies and within them, and a powerful tool for studying linked character between energy and mineral industries and related industries, carbon emission, environmental protection and so on from the perspective of physical economics. Based on document analysis, this paper introduces the concept and theoretical basis of the input-output network and energy and mineral industries, and then systematically expounds the research status of the input-output network from several dimensions such as data source, research object, and research question. Finally, the paper summarizes research methods, research objects, and application scope of the input-output network, points out the weak links, and prospects some future development directions in energy and mineral industries.
A comparison of intertidal metazoan biodiversity among different salinity zones of Louisiana coastal marshes
Authors: Husseneder Claudia , Rayle Patrick , Aker Benjamin G. , Thomas W. Kelley , Foil Lane D.
Published: September 2022

Tidal marshes are among the most valuable, productive, and vulnerable ecosystems with high biodiversity. Louisiana’s saltmarshes are endangered by natural and man-made stressors, including oil pollution, saltwater intrusion, and land loss due to sea level rise and erosion. Freshwater diversions have been planned to restore sediment input from the Mississippi River to rebuild marsh habitats in South Louisiana. These proposed diversions will undoubtedly change salinity levels, which is a major controlling factor in the distribution of marsh organisms, including those in soil; however, detailed pre-event inventories are lacking. This study describes the diversity of metazoan meiofauna (organisms between 45 and 500 μm) and environmental DNA in marsh soil collected in 2018 from Barataria and Caillou Bay, Louisiana, across three salinity zones and four distances from the marsh edge. Diversity analyses using 18S rRNA gene metabarcodes identified salinity as a factor impacting soil metazoan composition. Nematoda and Mollusca were equally distributed across salinity zones. Gastrotricha, Bryozoa, Rotifera, and Platyhelminthes were more prevalent in low salinity while Kinorhyncha were not detected in low salinity. Annelida and insects were equally common in low and high salinity but less in mid salinity. Five nematodes (Eumonhystera filiformis, two Prismatolaimus spp., Anoplostoma sp., and Prodorylaimus sp.), two annelids (Marionina southerni and Dendronereis aestuarina), two platyhelminthes (Rhynchoscolex simplex and Olisthanella truncula), the gastrotrich Chaetonotus novenarius and four collembola and ostracods appear to be low salinity bioindicators and are expected to expand range with freshwater diversions. No frequently detected organisms were unique to mid or high salinity zones, but four Nematoda (Meleidogyne spartinae, Prochaetosoma sp., Halalaimus sp., and Dichromadora sp.), two Annelida (Alitta succinea and Namalycastis jaya), two Platyhelminthes (Macrostomum kepneri and Mesorhynchus terminostylis), and one Kinorhyncha (Echinoderes sp.) were never detected in low salinity zones. None of the frequently detected taxa were unique for a particular distance from the marsh edge or bay. This dataset will be useful as baseline for assessing how soil communities will change in response to salinity changes caused by freshwater diversions and saltwater intrusion as well as measuring the environmental impact of pollution and other stressors.
Carbon neutrality vs. neutralité carbone: A comparative study on French and English users’ perceptions and social capital on Twitter
Authors: Yao Qi , Li Rita Yi Man , Song Lingxi
Published: September 2022

Carbon neutrality is one of the most critical global concerns at present. As one of the largest social media, Twitter is used widely by individuals, organisations, and government agencies to share their comments and perceptions on carbon neutrality. This study collected 26425 English and 20331 French tweets to compare the differences between French and English tweets. Social network analysis found that users in the French social networks interacted more frequently than the English ones. The geodesic analysis evidenced that the connection of any two users required about five intermediate users on average in French networks, while English ones required seven intermediate users. The modularity metrics of the English network were higher, indicating that users in English networks did not communicate with different clusters and people in carbon neutrality issues. In addition, the French network of carbon neutrality activists comprised politicians, government agencies, journalists, NGOs, and companies, while those in the English network mainly included companies, media, and politicians. Sentiment analysis and independent samples t-test have confirmed that despite the types of activists and the interactions between clusters being different, negative Tweets were more than positive ones in English and French networks, especially in French networks. It may be caused by people’s dissatisfaction with the government’s current carbon neutrality policy. By analysing the social pattern on Twitter, the research results allow people to know more about the means to enhance carbon-neutral knowledge sharing, which has the policy and social significance for addressing climate change.
Spatiotemporal characteristics and influencing factors of agricultural low-carbon economic efficiency in china
Authors: Ma Dalai , Xiao Yaping , Zhang Fengtai , Zhao Na , Xiao Yuedong , Chuai Xiaowei
Published: September 2022

Developing low-carbon agriculture can effectively avoid the waste of natural resources, thus contributing to the long-term sustainability of agriculture. This study uses the Super-SBM model to measure agricultural low-carbon economic efficiency (ALEE) in China from 2000 to 2018, then analyzes the spatial-temporal evolution characteristics. Simultaneously, the influencing factors of ALEE are investigated using spatial econometric model. The results show that: (1) In terms of temporal evolution, the ALEE in most provinces is declined over time, with only a few provinces improving. The ALEE in China and the three regions all show an obvious “L” trend of decline first and then stability. (2) From the perspective of spatial differentiation, provinces in eastern region have higher ALEE, while those in central and western regions have lower ALEE. Hainan’s ALEE has an absolute advantage, while Shanxi is the worst. (3) China’s ALEE shows obvious spatial agglomeration characteristics of H-H and L-L agglomeration, which are further enhanced over time. The number of L-L agglomeration provinces gradually increases, indicating that China’s ALEE has not been improved significantly. (4) Economic growth level and Agricultural scientific and Technological progress have effectively improved the ALEE. However, Capital deepening, Government fiscal expenditure, Agricultural planting structure, and Agricultural disaster all have negative impacts. Rural electricity consumption also has a negative impact, but the impact is not significant. To accelerate the development of low-carbon agriculture, all regions must not only pursue a differentiated low-carbon agriculture development path, but also accelerate agricultural transformation, strengthen research and development, and popularize low-carbon agricultural technologies, reduce the input of traditional agricultural means of production, optimize the agricultural industrial structure, and adjust agricultural subsidy policies.
Simulating the freezing-thawing processes based on MODIS data in the Three-River Souce Region, China
Authors: Ju Qin , Zhang Rongrong , Wang Guoqing , Hao Wenlong , Wang Qin , Liu Yanli , Wang Wei
Published: September 2022

The processes of soil freezing-thawing lead to soil water and heat movement in cold regions, which significantly influences the hydrological and energy cycles in the soil-plant-atmosphere system. This study presents a soil water content coupled with heat transfer model based on physical processes of water and heat movement in frozen soil. The model was calibrated and validated using the measured data of soil temperature and frost and thaw depth at 19 stations in and around the Three-River Source Region of China. The results show that the frozen soil model could capture the processes of soil freezing-thawing processes well at this region. The relationship between model parameters and climate and vegetation factors was analyzed using the observation data and remote sensing data obtained from MODIS, and results showed that the parameter c which represents the soil properties has a good correlation with longitude and vegetation coverage. A multi-regression model was established to estimate the model parameters in regions without observation data and its determination coefficient R2 was 0.82. The mean relative error between calibration and inversion parameters of 19 stations is 6.29%. Thus, the proposed method can be applied to cold regions without observation data to obtain the parameters and simulated the soil freezing-thawing processes.
Integration of single-cell and bulk RNA-seq to establish a predictive signature based on the differentiation trajectory of M2 macrophages in lung adenocarcinoma
Authors: Chen Zhike , Yang Jian , Li Yu , Zeng Weibiao , Bai Yiling , Ding Cheng , Xu Chun , Li Chang , Chen Jun , Ju Sheng , Tang Lijuan , Zhao Jun
Published: September 2022

Background: Tumor-associated macrophages as important members of the tumor microenvironment, are highly plastic and heterogeneous. TAMs can be classified into two preliminary subtypes: M1 and M2 macrophages. M2 macrophages are significantly associated with the progression of lung adenocarcinoma. However, no study has investigated the heterogeneity among M2 macrophages and their differentiation-related genes at the single-cell level to guide the clinical treatment of lung adenocarcinoma.

Methods: Using the available annotation information from the Tumor Immune Single-cell Hub database, we clustered and annotated 12 lung adenocarcinoma samples using the R package ‘Seurat’. Subsequently, we extracted M2 macrophages for secondary clustering analysis and performed cell trajectory analysis using the R package ‘monocle2’. Based on heterogeneous genes associated with the differentiation trajectory of M2 macrophages, we established a prognostic lung adenocarcinoma model using Lasso-Cox and multivariate stepwise regression. In addition, we also performed immunotherapy and chemotherapy predictions.

Results: M2 macrophages exhibit heterogeneity among themselves. M2 macrophages in different differentiation states showed significant differences in pathway activation and immune cell communication. Prognostic signature based on heterogeneous genes can be used to classify the prognostic status and abundance of immune cell infiltration in lung adenocarcinoma patients. In addition, the calculation of the Tumor Immune Dysfunction and Exclusion (TIDE) algorithm and the validation of the GSE126044 database indicated that lung adenocarcinoma patients with high-risk scores had poorer treatment outcomes when receiving immune checkpoint inhibitors treatment.

Conclusion: Based on scRNA-seq and Bulk-seq data, we identified M2 macrophage-associated prognostic signature with a potential clinical utility to improve precision therapy.
Detection of BRCA1/2 pathogenic variants in patients with breast and/or ovarian cancer and their families. Analysis of 3,458 cases from Lower Silesia (Poland) according to the diagnostic algorithm of the National Cancer Control Programme
Authors: Doraczynska-Kowalik Anna , Michalowska Dagmara , Matkowski Rafal , Czykalko Ewelina , Blomka Dorota , Semeniuk Mariola , Abrahamowska Mariola , Janus-Szymanska Gabriela , Mlynarczykowska Paulina , Szynglarewicz Bartlomiej , Pawlak Ireneusz , Maciejczyk Adam , Laczmanska Izabela
Published: September 2022

Breast and ovarian cancers are among the most common malignancies in the female population, with approximately 5–10% of cases being hereditary. BRCA1 and BRCA2 with other homologous recombination genes are the most tested genes in hereditary breast and ovarian cancer (HBOC) patients. As next-generation sequencing (NGS) has become a standard and popular technique, such as for HBOC, it has greatly simplified and accelerated molecular diagnosis of cancer. The study group included 3,458 HBOC patients or their relatives from Lower Silesia (Poland) (a voivodeship located in south-west Poland inhabited by 2.9 million people). All patients were tested according to the recommendations from the National Cancer Control Programme of the Ministry of Health for the years 2018–21. We tested 3,400 patients for recurrent pathogenic variants for the Polish population: five BRCA1 founder variants (c.5266dup, c.181T>G, c.4035del, c.3700_3704del, and c.68_69del), two PALB2 variants (c.509_510del, c.172_175del) and three CHEK2 variants [c.1100del, c.444+1G>A, g.27417113-27422508del (del5395)]. Next 260 patients from the study group were chosen for the BRCA1/2 NGS panel, and additionally selected marker pathogenic variants were tested using Sanger sequencing and MLPA methods in 45 and 13 individuals, respectively. The analysis of BRCA1/2 in the 3,458 patients with HBOC or their relatives revealed 144 carriers of 37 different pathogenic variants (22 in BRCA1 and 15 in BRCA2). Among all detected variants, 71.53% constituted founder pathogenic BRCA1 variants. Our study has revealed that for the Lower Silesian population, the first-line BRCA1/2 molecular test may be limited to only three variants in BRCA1—c.5266dup, c.181T>G, and c.4035del—but the aim should be to provide a full screening test of HBOC critical genes. The key and still growing role of molecular diagnostics of neoplasms, which includes HBOC, is undeniable. Therefore, it is necessary to provide complete and optimal therapeutic and prophylactic algorithms in line with current medical knowledge.
Identification of a apoptosis-related LncRNA signature to improve prognosis prediction and immunotherapy response in lung adenocarcinoma patients
Authors: Luo Ting , Yu Shiqun , Ouyang Jin , Zeng Fanfan , Gao Liyun , Huang Shaoxin , Wang Xin
Published: September 2022

Apoptosis is closely associated with the development of various cancers, including lung adenocarcinoma (LUAD). However, the prognostic value of apoptosis-related lncRNAs (ApoRLs) in LUAD has not been fully elucidated. In the present study, we screened 2, 960 ApoRLs by constructing a co-expression network of mRNAs-lncRNAs associated with apoptosis, and identified 421 ApoRLs that were differentially expressed between LUAD samples and normal lung samples. Sixteen differentially expressed apoptosis-related lncRNAs (DE-ApoRLs) with prognostic relevance to LUAD patients were screened using univariate Cox regression analysis. An apoptosis-related lncRNA signature (ApoRLSig ) containing 10 ApoRLs was constructed by applying the Least Absolute Shrinkage and Selection Operator (LASSO) Cox regression method, and all LUAD patients in the TCGA cohort were divided into high or low risk groups. Moreover, patients in the high-risk group had a worse prognosis (p < 0.05). When analyzed in conjunction with clinical features, we found ApoRLSig to be an independent predictor of LUAD patients and established a prognostic nomogram combining ApoRLSig and clinical features. Gene set enrichment analysis (GSEA) revealed that ApoRLSig is involved in many malignancy-associated immunomodulatory pathways. In addition, there were significant differences in the immune microenvironment and immune cells between the high-risk and low-risk groups. Further analysis revealed that the expression levels of most immune checkpoint genes (ICGs) were higher in the high-risk group, which suggested that the immunotherapy effect was better in the high-risk group than in the low-risk group. And we found that the high-risk group was also better than the low-risk group in terms of chemotherapy effect. In conclusion, we successfully constructed an ApoRLSig which could predict the prognosis of LUAD patients and provide a novel strategy for the antitumor treatment of LUAD patients.
Development and validation of a novel necroptosis-related score to improve the outcomes of clear cell renal cell carcinoma
Authors: Chen Ji , Tao Qiqi , Lang Zhichao , Jin Yan , Chen Guanqi , Li Xinling , Yu Zhixian , Li Yeping
Published: September 2022

Necroptosis has been indicated as a key regulator of tumor progression. However, the prognostic regulatory role of necroptosis in clear cell renal cell carcinoma (ccRCC) needs to be further investigated. In this study, necroptosis-related subtypes were identified by mining the public cohort (n = 530) obtained from The Cancer Genome Atlas. By applying Principal Component Analysis (PCA), the necroptosis-related scores (N-Score) were developed to assess the prognosis procession of ccRCC. The results were further validated by an external clinical cohort (n = 116) obtained from the First Affiliated Hospital of Wenzhou Medical University. It has been found that N-Score could precisely distinguish the prognostic outcomes of patients as an independent risk factor (Hazard ratio = 4.990, 95% confidence interval (CI) = 2.007–12.403, p < 0.001). In addition, changes in N-Score were associated with differences in tumor mutational burden as well as immune infiltration characterization. Moreover, higher N-Scores were also correlated significantly molecular drug sensitivity and stronger immune checkpoint activity. Notably, the prognosis of ccRCC could be effectively guided by combining the N-Scores and external clinical indicators. In conclusion, N-Scores could be served as a robust and effective biomarker to improve the prognosis outcomes and targeted therapy of ccRCC.
Innate immune evasion by alphaviruses
Authors: Liu Yihan , Yuan Yupei , Zhang Leiliang
Published: September 2022

Alphaviruses contain many human and animal pathogens, such as CHIKV, SINV, and VEEV. Accumulating evidence indicates that innate immunity plays an important role in response to alphaviruses infection. In parallel, alphaviruses have evolved many strategies to evade host antiviral innate immunity. In the current review, we focus on the underlying mechanisms employed by alphaviruses to evade cGAS-STING, IFN, transcriptional host shutoff, translational host shutoff, and RNAi. Dissecting the detailed antiviral immune evasion mechanisms by alphaviruses will enhance our understanding of the pathogenesis of alphaviruses and may provide more effective strategies to control alphaviruses infection.