bluuebunny/crossref_metadata_2025 huggingface dataset:
Tangled crises in Turkana: investigating the spread of Prosopis in Kenya’s northern drylands
Authors: Derbyshire Samuel F. , Leakey Acacia , Lowasa Lucas
Published: March 2024

Following a severe drought in the late 1970s and early 1980s, the invasive shrub Prosopis, a kind of mesquite native to South America, was introduced by international organizations to locations across Kenya’s drylands, including the Turkana region in the far north.
Prosopis, known as etirae in Turkana, was envisaged as a solution to a range of problems, including deforestation, fuelwood shortages and general environmental deterioration. While exacerbated by drought, these problems were perceived to reflect a much more fundamental crisis, with prevalent views at the time envisaging pastoralism as unsustainable, destructive and in need of overhaul – a narrative dating to colonial times that has since been discredited. Since its introduction, etirae has spread relentlessly, invading riparian land, encroaching on cultivation plots and growing to new heights and thicknesses. Investigating its entanglements with Turkana livelihoods and economic relationships is also a process of understanding how it has braided its way through contested processes of social change, and how it has come to be intertwined with conceptions of cascading crisis quite distinct from the narratives that led to its initial introduction. Implications emerge regarding both the complicated biological residues of past development interventions and the totalizing crisis-oriented narratives that shape drylands development in the current era of climate change.
African universities and the challenge of postcolonial development
Authors: Arowosegbe Jeremiah O.
Published: March 2024

Extensively ignored by the literature on the subject, recent interest in the fate of academic freedom in Africa is linked with shared concerns about the exploding nature of its societal crises. The collapse of political integration and social cohesion; the decline of the civil society and the implosion of conflicts; the rise of authoritarian, non-developmental populist regimes amid extreme poverty; and the worsening material conditions of the populations are major indications of such crises. Nowhere are these crises worse illustrated than in the universities where constrained funding, infrastructural collapse, massive brain drains and strained relations with the state inhibit the production of knowledge. This article reflects on the trajectory of the universities in postcolonial Africa. It draws on the national public universities in Nigeria and accounts for the changes and continuities underlying their performance against the backdrop of hostile material conditions and uncongenial political control, which not only remain disruptive but continue to undermine institutional autonomy and the integrity of scholarship in the universities across Africa.
Optimal growth and development: are teenagers getting enough micronutrients from their diet?
Authors: Walsh Niamh M. , Flynn Albert , Walton Janette , Kehoe Laura
Published: March 2024

The teenage years represent a crucial period of physical and cognitive growth and development with sufficient micronutrient intakes necessary to meet high nutritional requirements. This review examines current micronutrient intakes in teenagers in the Western world in the context of public health implications including the prevalence of inadequate intakes and risk of excessive intakes. Intakes of vitamins A, D, E and C, folate, calcium, iron, magnesium, zinc and potassium in teenagers are low when compared to generally accepted recommendations, while there is little risk of excessive micronutrient intakes based on current dietary patterns. Therefore, strategies should focus on increasing micronutrient intakes in order to decrease the risk of negative impacts resulting from these low intakes. These strategies should be mindful of guidance towards an environmentally sustainable diet whilst ensuring that nutrient intakes in teenagers are not further negatively impacted. In order to identify, implement and monitor the effectiveness of such strategies, intakes of micronutrients should be continually monitored in nationally representative samples of the population for all age groups including this vulnerable cohort of teenagers.
Decision making capacity for treatment in psychiatric inpatients: a systematic review and meta-analysis
Authors: Marcó-García Silvia , Ariyo Kevin , Owen Gareth S. , David Anthony S.
Published: March 2024

Decision-making capacity (DMC) among psychiatric inpatients is a pivotal clinical concern. A review by Okai et al. (2007) suggested that most psychiatric inpatients have DMC for treatment, and its assessment is reliable. Nevertheless, the high heterogeneity and mixed results from other studies mean there is considerable uncertainty around this topic. This study aimed to update Okai's research by conducting a systematic review with meta-analysis to address heterogeneity. We performed a systematic search across four databases, yielding 5351 results. We extracted data from 20 eligible studies on adult psychiatric inpatients, covering DMC assessments from 2006 to May 2022. A meta-analysis was conducted on 11 papers, and a quality assessment was performed. The study protocol was registered on PROSPERO (ID: CRD42022330074). The proportion of patients with DMC for treatment varied widely based on treatment setting, the specific decision and assessment methods. Reliable capacity assessment was feasible. The Mini-Mental State Examination (MMSE), Global Assessment of Function (GAF), and Brief Psychiatric Rating Scale (BPRS) predicted clinical judgments of capacity. Schizophrenia and bipolar mania were linked to the highest incapacity rates, while depression and anxiety symptoms were associated with better capacity and insight. Unemployment was the only sociodemographic factor correlated with incapacity. Assessing mental capacity is replicable, with most psychiatric inpatients able to make treatment decisions. However, this capacity varies with admission stage, formal status (involuntary or voluntary), and information provided. The severity of psychopathology is linked to mental capacity, though detailed psychopathological data are limited.
POMPEY'S APULIAN ESTATES
Authors: Small Alastair
Published: March 2024

Pompey owned numerous properties in Italy, but except for a few residential villas little is known of their location or economic function. However, two amphora stamps have been attributed to him, which show that he was involved in the manufacture of amphorae, and probably in the production of the wine. Four tile stamps, found in the vicinity of Gravina in Puglia and at a villa at San Gilio in the upper Bradano valley, can also be attributed to Pompey for reasons discussed in detail in this paper. Since they were found in locations close to a drove road, it is inferred that Pompey had invested in properties which could be used as pasture for transhumant sheep. The circumstances in which he acquired the estate near Gravina are discussed, and it is suggested that it fell within the territory of Silvium and is likely to have been acquired by him after the War of Spartacus in which the settlement was destroyed. It is also suggested that the estate was bought by Octavian in the sale of Pompey's properties, which was still ongoing in 44 BC.
Wages and Labor Relations during Francoist Developmentalism: The Role of the New Unionism
Authors: Cardenas Luis
Published: March 2024

The relation between wage growth and the reconstruction of the labor movement in Spain, during the developmentalist Francoist regime (1957-1975), has been controversial. Applying the power resources theory in order to explain wage growth, this paper argues that the rise of the labor share was the result of the increased bargaining power of workers in spite of repressive policies of the Francoist government. The action of new trade unionism mobilized the following power resources. First, the structural power increased as a consequence of techno-economics transformations of Fordism and a generational change of the labor force. Second, the associational power increased due to new organizational forms, with more democratic modes of collective action and combining clandestine and legal actions, and the reappearance of collective bargaining with the Law of Collective Bargaining Agreements of 1958. These political opportunities opened up the possibility to the new trade unionist of developing the strategy of entryism that served as the driving force for worker mobilization.

Consequently, the new trade unionists could mobilize their power resources in the three spheres, exchange, production, and politics, as a strategy by accelerating collaborative action, establishing a discursive and cultural framework, and allowing the articulation of the different trade union actions. In this way, internal solidarity was favored when a collective agreement was negotiated at the company or sector level (and not an individual contract, as it has been to date), due to the links with the social activities characteristic of the paternalistic management of labor relations and the presence of anti-Francoist organizations, the “network embeddedness” was strengthened. In turn, the successes in the consequence of wage increases and solidarity with other workers who were suffering repression increased the narrative resources that frame union actions. All this made it possible to counterbalance in part for the restrictions imposed by the dictatorship, which weakened the structural power of workers in the political sphere.

The main contribution to the existing knowledge on the issue consists in explaining the apparent paradox between the wage policy of the Francoist government (oriented towards wage restriction) and the increase in labor share. This also serves to understand how labor markets and the wage-setting mechanism worked from a historical point of view. As a corollary, the previous analysis implies that the most important factor was the ability to mobilize workers successfully. The union strategy that combined democratic instruments of representation (assemblies) with clandestine and legal actions served as a catalyst for other productive and social changes. This reflects the primacy of the action of social actors for the worker mobilization theory.
The double jeopardy of low family income and negative emotionality: The family stress model revisited
Authors: Şengül-İnal Gülbin , Borgen Nicolai Topstad , Dearing Eric , Zachrisson Henrik Daae
Published: March 2024

The family stress model has, for decades, guided empirical work linking poverty with increased risk of child social-emotional dysfunction. The present study extends this line of work by examining whether child negative emotionality moderates associations between family income, family stress (maternal distress, parental locus of control, and relationship dissatisfaction), and later externalizing and internalizing behavior problems. In a longitudinal population-based sample (n ~ 80,000) of Norwegian children followed from birth through age five (The Norwegian Mother, Father, and Child Cohort Study; MoBa), we examined whether high (vs. moderate or low) negative emotionality families would display: (a) compounding stress (i.e., particularly strong associations between low family income and family stress), (b) diathesis-stress (i.e., particularly strong associations between family stress and behavior problems), or (c) double jeopardy (i.e., both compounding stress and diathesis-stress moderating effects). Negative emotionality significantly moderated the association between family income and behavior problems in a manner most consistent with double jeopardy. As a result, compared with children with moderate/low negative emotionality, the family income-behavior problems association was two to three times larger for those with higher negative emotionality. These findings underscore the active role children may play in family processes that link low family income with behavior problems.
Potting communities and conservatism in the Purépecha empire at Angamuco, Michoacán, Mexico
Authors: Cohen Anna S.
Published: March 2024

Stylistic shifts in ceramics are often linked with sociopolitical changes, yet adopting new ceramic designs may indicate anything from shared aesthetic appeal to emulation. Focusing on multiple ceramic technologies is critical for understanding ceramic changes as they relate to wider social fluctuations. The relationships between clay and ceramic recipes, and communities of practice, have not been studied at the urban landscape of Angamuco, Michoacán in western Mexico. Petrographic and geochemical analyses of tempers and fabrics indicate that ceramics, including imperial Purépecha (a.d. 1350–1530) vessels, were created from local and regional materials, and that these materials remained relatively stable for over 1,000 years. Ceramic archaeometry in western Mexico has been relatively limited compared to other parts of Mesoamerica, and this study may be compared to future studies in the region.
High-precision strong lensing models of galaxy clusters in the JWST era
Authors: Bergamini P. , Grillo C. , Rosati P. , Acebron A. , Vanzella E. , Mercurio A. , Meneghetti M. , Angora G. , Granata G. , Mestric U. , Caminha G. B. , Treu T.
Published: March 2024

We present high-precision strong lensing models for the galaxy clusters MACS J0416.1–0403 at z=0.396 and Abell 2744 at z=0.307. The models are constrained by two of the largest data-sets of secure multiple images ever used in lensing. These are identified from the photometric images observed by the Hubble space telescope and JWST in combination with spectroscopic data obtained by the Multi-Unit Spectroscopic Explorer at the Very Large Telescope. The same spectro-photometric data are used to create pure and complete samples of cluster member galaxies. Our models allow an extremely precise estimation of the cluster total mass distribution and produce accurate magnification maps that are fundamental to study the physical properties (mass, size, luminosity, etc.) of the lensed high-redshift galaxies.
Publicly funded health insurance schemes and demand for health services: evidence from an Indian state using a matching estimator approach
Authors: Singh Vanita
Published: March 2024

Using Demographic and Health Survey data (2015–16) from the state of Andhra Pradesh, we estimate the differential probability of hysterectomy (removal of uterus) for women (aged 15–49 years) covered under publicly funded health insurance (PFHI) schemes relative to those not covered. To reduce the extent of selection bias into treatment assignment (PFHI coverage) we use matching methods, propensity score matching, and coarsened exact matching, achieving a comparable treatment and control group. We find that PFHI coverage increases the probability of undergoing a hysterectomy by 7–11 percentage points in our study sample. Sub-sample analysis indicates that the observed increase is significant for women with lower education levels and higher order parity. Additionally, we perform a test of no-hidden bias by estimating the treatment effect on placebo outcomes (doctor's visit, health check-up). The robustness of the results is established using different matching specifications and sensitivity analysis. The study results are indicative of increased demand for surgical intervention associated with PFHI coverage in our study sample, suggesting a need for critical evaluation of the PFHI scheme design and delivery in the context of increasing reliance on PFHI schemes for delivering specialised care to poor people, neglect of preventive and primary care, and the prevailing fiscal constraints in the healthcare sector.
Effects of Stress‐Driven Melt Segregation on Melt Orientation, Melt Connectivity and Anisotropic Permeability
Authors: Bader James , Zhu Wenlu , Montési Laurent , Qi Chao , Cordonnier Benoit , Kohlstedt David , Warren Jessica
Published: March 2024

Stress‐driven melt segregation may have important geochemical and geophysical effects but remains a poorly understood process. Few constraints exist on the permeability and distribution of melt in deformed partially molten rocks. Here, we characterize the 3D melt network and resulting permeability of an experimentally deformed partially molten rock containing several melt‐rich bands based on an X‐ray microtomography data set. Melt fractions range from 0.08 to 0.28 in the ∼20‐μm‐thick melt‐rich bands, and from 0.02 to 0.07 in the intervening ∼30‐μm‐thick regions. We simulated melt flow through subvolumes extracted from the reconstructed rock at five length scales ranging from the grain scale (3 μm) to the minimum length required to fully encompass two melt‐rich bands (64 μm). At grain scale, few subvolumes contain interconnected melt, and permeability is isotropic. As the length scale increases, more subvolumes contain melt that is interconnected parallel to the melt bands, but connectivity diminishes in the direction perpendicular to them. Even if melt is connected in all directions, permeability is lower perpendicular to the bands, in agreement with the elongation of melt pockets. Permeability parallel to the bands is proportional to melt fraction to the power of an exponent that increases from ∼2 to 5 with increasing length scale. The permeability in directions parallel to the bands is comparable to that for an isotropic partially molten rock. However, no flow is possible perpendicular to the bands over distances similar to the band spacing. Melt connectivity limits sample scale melt flow to the plane of the melt‐rich bands.
Comment on “Comparison of the Efficiencies of the Prognostic Generalized Complementary Functions on Evaporation Estimation” by Wang, L., et al., Published in Journal of Geophysical Research: Atmospheres
Authors: Szilagyi Jozsef
Published: March 2024

Surprising significant underperformance of the polynomial complementary relationship (PCR) of evaporation (Szilagyi et al., 2017, https://doi.org/10.1002/2016jd025611) by Wang et al. (2023, https://doi.org/10.1029/2023jd038683) is caused by the (a) station‐by‐station application of a grid‐based estimation procedure of the Priestley‐Taylor parameter (α) value, and (b) choice of the wind function. Application of the Rome wind function in the Penman equation together with either a well‐chosen single (constant) α or α as a function of the wet‐environment air temperature, should result in much improved evaporation estimates by the PCR in line with previous studies.
Topological Dirac-vortex microcavity laser for robust on-chip optoelectronics
Authors: Wu Yuanpeng , Mi Zetian
Published: March 2024

Dirac-vortex microcavity laser based on InAs/InGaAs quantum dots have been experimentally realized on silicon substrate. The topological laser features a large spectral range and high robustness against variations such as cavity size.
Less common phenotypes of myelin oligodendrocyte glycoprotein antibody-related diseases in children deserve more attention
Authors: Wang Xiao-yu , Jiang Yan , Wu Peng , Ma Jian-nan , Yuan Ping , Li Xiu-juan , Jiang Li
Published: March 2024

Background

To facilitate the identification of less common clinical phenotypes of myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) in children.

Methods

We retrospectively reviewed medical records of 236 patients with MOGAD. The following phenotypes were considered to be typical for MOGAD: ADEM, ON, TM, and NMOSD. Less common onset clinical phenotypes were screened out; their clinical and magnetic resonance imaging (MRI), diagnosis, treatment, and prognosis were summarized and analyzed.

Results

16 cases (6.8%) presented as cortical encephalitis, with convulsions, headache, and fever as the main symptoms. 15 cases were misdiagnosed in the early period. 13 cases (5.5%) showed the overlapping syndrome of MOGAD and anti-N-methyl-D aspartate receptor encephalitis (MNOS), with seizures (92.3%) being the most common clinical symptom. 11 cases (84.6%) showed relapses. The cerebral leukodystrophy-like phenotype was present in seven cases (3.0%), with a recurrence rate of 50%. Isolated seizures without any findings on MRI phenotype was present in three cases (1.3%), with the only clinical symptom being seizures of focal origin. Three cases (1.3%) of aseptic meningitis phenotype presented with prolonged fever.

Conclusion

40/236 (16.9%) of children with MOGAD had less common phenotypes. Less common clinical phenotypes of pediatric MOGAD are susceptible to misdiagnosis and deserve more attention.

Impact

This is the first comprehensive analysis and summary of all less commonl clinical phenotypes of MOGAD in children, while previous studies have only focused on a specific phenotype or case reports.

We analyzed the characteristics of MOGAD in children and further revealed the reasons why these less common clinical phenotypes are prone to misdiagnosis and deserve more attention.

Our research on treatment has shown that early detection of MOG antibodies and early treatment are of great significance for improving the prognosis of these patients.
Effects of hemodynamic alterations and oxygen saturation on cerebral perfusion in congenital heart disease
Authors: De Silvestro Alexandra , Natalucci Giancarlo , Feldmann Maria , Hagmann Cornelia , Nguyen Thi Dao , Coraj Seline , Jakab Andras , Kottke Raimund , Latal Beatrice , Knirsch Walter , Tuura Ruth
Published: March 2024

Background

Patients with severe congenital heart disease (CHD) are at risk for neurodevelopmental impairment. An abnormal cerebral blood supply caused by the altered cardiac physiology may limit optimal brain development. The aim of this study was to evaluate the effect of a systemic-to-pulmonary shunt, aortic arch obstruction and arterial oxygen saturation on cerebral perfusion in patients with severe CHD.

Methods

Patients with severe CHD requiring cardiac surgery within the first six weeks of life, who underwent pre- and/or postoperative brain magnetic resonance imaging (MRI), and healthy controls with one postnatal scan were included. Cerebral perfusion in deep and cortical gray matter was assessed by pseudocontinuous arterial spin labeling MRI.

Results

We included 59 CHD and 23 healthy control scans. The presence of a systemic-to-pulmonary shunt was associated with decreased perfusion in cortical (p = 0.003), but not in deep gray matter (p = 0.031). No evidence for an effect of aortic arch obstruction and arterial oxygen saturation on cerebral perfusion was found. After adjusting for hemodynamic and oxygen saturation parameters, deep (p = 0.018) and cortical (p = 0.012) gray matter perfusion was increased in patients with CHD compared to controls.

Conclusion

We detected regional differences in compensation to the cerebral steal effect in patients with severe CHD.

Impact

Patients with severe congenital heart disease (CHD) have altered postnatal brain hemodynamics.

A systemic-to-pulmonary shunt was associated with decreased perfusion in cortical gray matter but preserved perfusion in deep gray matter, pointing towards regional differences in compensation to the cerebral steal effect.

No effects of aortic arch obstruction and arterial oxygenation on cerebral perfusion were seen.

Cerebral perfusion was increased in patients with CHD compared to healthy controls after adjusting for hemodynamic alterations and oxygen saturation.

To improve neuroprotection and neurodevelopmental outcomes, it is important to increase our understanding of the factors influencing cerebral perfusion in neonates with severe CHD.
Management of neurogenic bladder in patients with spinal cord injuries/disorders and end stage renal disease: a case series
Authors: Chaggar Rajbir , Goetz Lance L. , Adler Jordan , Bhuiyan Mohammed B. A. , McAvoy Sean , Tubbs Jeffrey
Published: March 2024

Introduction

Patients with spinal cord injuries/disorders (SCI/D) often suffer from bladder dysfunction, commonly referred to as neurogenic bladder or neurogenic lower urinary tract dysfunction (NLUTD). Standard urologic evaluation and management help to minimize complications such as vesicoureteral reflux, urinary tract infection, and nephrolithiasis. However, we have further encountered patients with more complex issues, such as chronic kidney disease (CKD), end-stage renal disease (ESRD), bilateral nephrectomies, and urinary diversion/augmentation surgeries. Of particular interest, there is a lack of standardized guidance for bladder management in SCI/D patients with ESRD. These patients are at high risk for urological complications and would benefit from codified bladder management strategies.

Case presentation

In this article, we present eleven unique cases of NLUTD with associated ESRD and discuss recommendations utilizing simple and commonly available clinical interventions.

Discussion

The inherently small population size of SCI/D patients with NLUTD and ESRD makes detailing a large sample size case series difficult. Future studies must aim to include a larger sample size as able, however, to better determine standardized protocols for chronic bladder management in SCI/D patients with NLUTD and ESRD. Experiences from this small case series are offered for consideration.
Hot metacognition: poorer metacognitive efficiency following acute but not traumatic stress
Authors: Smith Alicia J. , Bisby James A. , Dercon Quentin , Bevan Anna , Kigar Stacey L. , Lynall Mary-Ellen , Dalgleish Tim , Hitchcock Caitlin , Nord Camilla L.
Published: March 2024

Aberrations to metacognition—the ability to reflect on and evaluate self-performance—are a feature of poor mental health. Theoretical models of post-traumatic stress disorder propose that following severe stress or trauma, maladaptive metacognitive evaluations and appraisals of the event drive the development of symptoms. Empirical research is required in order to reveal whether disruptions to metacognition cause or contribute to symptom development in line with theoretical accounts, or are simply a consequence of ongoing psychopathology. In two experiments, using hierarchical Bayesian modelling of metacognition measured in a memory recognition task, we assessed whether distortions to metacognition occur at a state-level after an acute stress induction, and/or at a trait-level in a sample of individuals experiencing intrusive memories following traumatic stress. Results from experiment 1, an in-person laboratory-based experiment, demonstrated that heightened psychological responses to the stress induction were associated with poorer metacognitive efficiency, despite there being no overall change in metacognitive efficiency from pre- to post-stress (N = 27). Conversely, in experiment 2, an online experiment using the same metamemory task, we did not find evidence of metacognitive alterations in a transdiagnostic sample of patients with intrusive memory symptomatology following traumatic stress (N = 36, compared to 44 matched controls). Our results indicate a relationship between state-level psychological responses to stress and metacognitive alterations. The lack of evidence for pre- to post-stress differences in metamemory illustrates the importance for future studies to reveal the direction of this relationship, and consequently the duration of stress-associated metacognitive impairments and their impact on mental health.
Metabolic remodeling by the PD-L1 inhibitor BMS-202 significantly inhibits cell malignancy in human glioblastoma
Authors: Yang Xueou , Wang Wenjun , Ji Tianhai
Published: March 2024

Recently, crystallographic studies have demonstrated that BMS-202, a small-molecule compound characterized by a methoxy-1-pyridine chemical structure, exhibits a high affinity to PD-L1 dimerization. However, its roles and mechanisms in glioblastoma (GBM) remain unclear. The objective of this study is to investigate the antitumor activity of BMS-202 and its underlying mechanisms in GBM using multi-omics and bioinformatics techniques, along with a majority of in vitro and in vivo experiments, including CCK-8 assays, flow cytometry, co-immunoprecipitation, siRNA transfection, PCR, western blotting, cell migration/invasion assays and xenografts therapeutic assays. Our findings indicate that BMS-202 apparently inhibits the proliferation of GBM cells both in vitro and in vivo. Besides, it functionally blocks cell migration and invasion in vitro. Mechanistically, it reduces the expression of PD-L1 on the surface of GBM cells and interrupts the PD-L1-AKT-BCAT1 axis independent of mTOR signaling. Taken together, we conclude that BMS-202 is a promising therapeutic candidate for patients with GBM by remodeling their cell metabolism regimen, thus leading to better survival.
Cancer associated fibroblasts-derived SULF1 promotes gastric cancer metastasis and CDDP resistance through the TGFBR3-mediated TGF-β signaling pathway
Authors: Fang Xingchao , Chen Damin , Yang Xinyu , Cao Xiaogang , Cheng Quan , Liu Kanghui , Xu Peng , Wang Yanjuan , Xu Jiafeng , Zhao Siguo , Yan Zhengyuan
Published: March 2024

SULF1 has been implicated in a number of malignancies. The function of SULF1 in gastric cancer is disputed. The objective of this study was to examine the role and underlying molecular mechanisms of SULF1 in the context of gastric cancer. We found that the expression of SULF1 was increased in gastric cancer, especially in cancer-associated fibroblasts. The overexpression of SULF1 was found to be significantly correlated with unfavorable prognosis among individuals diagnosed with gastric cancer. Functionally, cancer-associated fibroblasts-derived SULF1 served as a oncogenic molecule which facilitated gastric cancer cells metastasis and CDDP resistance. Mechanistically, SULF1 regulated the communication between gastric cancer cells and cancer-associated fibroblasts in tumor microenvironment as a signaling molecule. Cancer-associated fibroblasts-secreted SULF1 interfered with the interaction between TGF-β1 and TGFBR3 by combining with TGFBR3 on gastric cancer cell membrane, subsequently activated TGF-β signaling pathway. In conclusion, our findings have presented novel approaches for potential treatment and prognosis prediction in individuals diagnosed with gastric cancer through the targeting of the CAFs-SULF1-TGFBR3-TGF-β1 signaling axis.
Targeting cell death pathways in intestinal ischemia-reperfusion injury: a comprehensive review
Authors: Wang Fei , Huang Huiming , Wei Xuejiao , Tan Peng , Wang Zhuguo , Hu Zhongdong
Published: March 2024

Intestinal ischemia-reperfusion (I/R) is a multifaceted pathological process, and there is a lack of clear treatment for intestinal I/R injury. During intestinal I/R, oxidative stress and inflammation triggered by cells can trigger a variety of cell death mechanisms, including apoptosis, autophagy, pyroptosis, ferroptosis, and necrosis. These cell death processes can send a danger signal for the body to be damaged and prevent intestinal I/R injury. Therefore, identifying key regulatory molecules or markers of these cell death mechanisms when intestinal I/R injury occurs may provide valuable information for the treatment of intestinal I/R injury. This paper reviews the regulatory molecules and potential markers that may be involved in regulating cell death during intestinal I/R and elaborates on the cell death mechanism of intestinal I/R injury at the molecular level to provide a theoretical basis for discovering new molecules or markers regulating cell death during intestinal I/R injury and provides ideas for drug development for the treatment of intestinal I/R injury.
Enhancing the fairness of AI prediction models by Quasi-Pareto improvement among heterogeneous thyroid nodule population
Authors: Yao Siqiong , Dai Fang , Sun Peng , Zhang Weituo , Qian Biyun , Lu Hui
Published: March 2024

Artificial Intelligence (AI) models for medical diagnosis often face challenges of generalizability and fairness. We highlighted the algorithmic unfairness in a large thyroid ultrasound dataset with significant diagnostic performance disparities across subgroups linked causally to sample size imbalances. To address this, we introduced the Quasi-Pareto Improvement (QPI) approach and a deep learning implementation (QP-Net) combining multi-task learning and domain adaptation to improve model performance among disadvantaged subgroups without compromising overall population performance. On the thyroid ultrasound dataset, our method significantly mitigated the area under curve (AUC) disparity for three less-prevalent subgroups by 0.213, 0.112, and 0.173 while maintaining the AUC for dominant subgroups; we also further confirmed the generalizability of our approach on two public datasets: the ISIC2019 skin disease dataset and the CheXpert chest radiograph dataset. Here we show the QPI approach to be widely applicable in promoting AI for equitable healthcare outcomes.
Hardware implementation of memristor-based artificial neural networks
Authors: Aguirre Fernando , Sebastian Abu , Le Gallo Manuel , Song Wenhao , Wang Tong , Yang J. Joshua , Lu Wei , Chang Meng-Fan , Ielmini Daniele , Yang Yuchao , Mehonic Adnan , Kenyon Anthony , Villena Marco A. , Roldán Juan B. , Wu Yuting , Hsu Hung-Hsi , Raghavan Nagarajan , Suñé Jordi , Miranda Enrique , Eltawil Ahmed , Setti Gianluca , Smagulova Kamilya , Salama Khaled N. , Krestinskaya Olga , Yan Xiaobing , Ang Kah-Wee , Jain Samarth , Li Sifan , Alharbi Osamah , Pazos Sebastian , Lanza Mario
Published: March 2024

Artificial Intelligence (AI) is currently experiencing a bloom driven by deep learning (DL) techniques, which rely on networks of connected simple computing units operating in parallel. The low communication bandwidth between memory and processing units in conventional von Neumann machines does not support the requirements of emerging applications that rely extensively on large sets of data. More recent computing paradigms, such as high parallelization and near-memory computing, help alleviate the data communication bottleneck to some extent, but paradigm- shifting concepts are required. Memristors, a novel beyond-complementary metal-oxide-semiconductor (CMOS) technology, are a promising choice for memory devices due to their unique intrinsic device-level properties, enabling both storing and computing with a small, massively-parallel footprint at low power. Theoretically, this directly translates to a major boost in energy efficiency and computational throughput, but various practical challenges remain. In this work we review the latest efforts for achieving hardware-based memristive artificial neural networks (ANNs), describing with detail the working principia of each block and the different design alternatives with their own advantages and disadvantages, as well as the tools required for accurate estimation of performance metrics. Ultimately, we aim to provide a comprehensive protocol of the materials and methods involved in memristive neural networks to those aiming to start working in this field and the experts looking for a holistic approach.
Trans-lesion synthesis and mismatch repair pathway crosstalk defines chemoresistance and hypermutation mechanisms in glioblastoma
Authors: Cheng Xing , An Jing , Lou Jitong , Gu Qisheng , Ding Weimin , Droby Gaith Nabil , Wang Yilin , Wang Chenghao , Gao Yanzhe , Anand Jay Ramanlal , Shelton Abigail , Satterlee Andrew Benson , Mann Breanna , Hsiao Yun-Chung , Liu Chih-Wei , Lu Kun , Hingtgen Shawn , Wang Jiguang , Liu Zhaoliang , Miller C. Ryan , Wu Di , Vaziri Cyrus , Yang Yang
Published: March 2024

Almost all Glioblastoma (GBM) are either intrinsically resistant to the chemotherapeutical drug temozolomide (TMZ) or acquire therapy-induced mutations that cause chemoresistance and recurrence. The genome maintenance mechanisms responsible for GBM chemoresistance and hypermutation are unknown. We show that the E3 ubiquitin ligase RAD18 (a proximal regulator of TLS) is activated in a Mismatch repair (MMR)-dependent manner in TMZ-treated GBM cells, promoting post-replicative gap-filling and survival. An unbiased CRISPR screen provides an aerial map of RAD18-interacting DNA damage response (DDR) pathways deployed by GBM to tolerate TMZ genotoxicity. Analysis of mutation signatures from TMZ-treated GBM reveals a role for RAD18 in error-free bypass of O6mG (the most toxic TMZ-induced lesion), and error-prone bypass of other TMZ-induced lesions. Our analyses of recurrent GBM patient samples establishes a correlation between low RAD18 expression and hypermutation. Taken together we define molecular underpinnings for the hallmark tumorigenic phenotypes of TMZ-treated GBM.
Dynamic mechanostereochemical switching of a co-conformationally flexible [2]catenane controlled by specific ionic guests
Authors: Yao Yueliang , Tse Yuen Cheong , Lai Samuel Kin-Man , Shi Yixiang , Low Kam-Hung , Au-Yeung Ho Yu
Published: March 2024

Responsive synthetic receptors for adaptive recognition of different ionic guests in a competitive environment are valuable molecular tools for not only ion sensing and transport, but also the development of ion-responsive smart materials and related technologies. By virtue of the mechanical chelation and ability to undergo large-amplitude co-conformational changes, described herein is the discovery of a chameleon-like [2]catenane that selectively binds copper(I) or sulfate ions and its associated co-conformational mechanostereochemical switching. This work highlights not only the advantages and versatility of catenane as a molecular skeleton in receptor design, but also its potential in constructing complex responsive systems with multiple inputs and outputs.
Structural basis for the modulation of MRP2 activity by phosphorylation and drugs
Authors: Mazza Tiziano , Roumeliotis Theodoros I. , Garitta Elena , Drew David , Rashid S. Tamir , Indiveri Cesare , Choudhary Jyoti S. , Linton Kenneth J. , Beis Konstantinos
Published: March 2024

Multidrug resistance-associated protein 2 (MRP2/ABCC2) is a polyspecific efflux transporter of organic anions expressed in hepatocyte canalicular membranes. MRP2 dysfunction, in Dubin-Johnson syndrome or by off-target inhibition, for example by the uricosuric drug probenecid, elevates circulating bilirubin glucuronide and is a cause of jaundice. Here, we determine the cryo-EM structure of rat Mrp2 (rMrp2) in an autoinhibited state and in complex with probenecid. The autoinhibited state exhibits an unusual conformation for this class of transporter in which the regulatory domain is folded within the transmembrane domain cavity. In vitro phosphorylation, mass spectrometry and transport assays show that phosphorylation of the regulatory domain relieves this autoinhibition and enhances rMrp2 transport activity. The in vitro data is confirmed in human hepatocyte-like cells, in which inhibition of endogenous kinases also reduces human MRP2 transport activity. The drug-bound state reveals two probenecid binding sites that suggest a dynamic interplay with autoinhibition. Mapping of the Dubin-Johnson mutations onto the rodent structure indicates that many may interfere with the transition between conformational states.
Discovery of orbital ordering in Bi2Sr2CaCu2O8+x
Authors: Wang Shuqiu , Kennedy Niall , Fujita Kazuhiro , Uchida Shin-ichi , Eisaki Hiroshi , Johnson Peter D. , Davis J. C. Séamus , O’Mahony Shane M.
Published: March 2024

The primordial ingredient of cuprate superconductivity is the CuO2 unit cell. Theories usually concentrate on the intra-atom Coulombic interactions dominating the 3d9 and 3d10 configurations of each copper ion. However, if Coulombic interactions also occur between electrons of the 2p6 orbitals of each planar oxygen atom, spontaneous orbital ordering may split their energy levels. This long-predicted intra-unit-cell symmetry breaking should generate an orbitally ordered phase, for which the charge transfer energy ε separating the 2p6 and 3d10 orbitals is distinct for the two oxygen atoms. Here we introduce sublattice-resolved ε(r) imaging to CuO2 studies and discover intra-unit-cell rotational symmetry breaking of ε(r). Spatially, this state is arranged in disordered Ising domains of orthogonally oriented orbital order bounded by dopant ions, and within whose domain walls low-energy electronic quadrupolar two-level systems occur. Overall, these data reveal a Q = 0 orbitally ordered state that splits the oxygen energy levels by ~50 meV, in underdoped CuO2.
Development of machine learning-based predictors for early diagnosis of hepatocellular carcinoma
Authors: Zhang Zi-Mei , Huang Yuting , Liu Guanghao , Yu Wenqi , Xie Qingsong , Chen Zixi , Huang Guanda , Wei Jinfen , Zhang Haibo , Chen Dong , Du Hongli
Published: March 2024

Hepatocellular carcinoma (HCC) remains a formidable malignancy that significantly impacts human health, and the early diagnosis of HCC holds paramount importance. Therefore, it is imperative to develop an efficacious signature for the early diagnosis of HCC. In this study, we aimed to develop early HCC predictors (eHCC-pred) using machine learning-based methods and compare their performance with existing methods. The enhancements and advancements of eHCC-pred encompassed the following: (i) utilization of a substantial number of samples, including an increased representation of cirrhosis tissues without HCC (CwoHCC) samples for model training and augmented numbers of HCC and CwoHCC samples for model validation; (ii) incorporation of two feature selection methods, namely minimum redundancy maximum relevance and maximum relevance maximum distance, along with the inclusion of eight machine learning-based methods; (iii) improvement in the accuracy of early HCC identification, elevating it from 78.15 to 97% using identical independent datasets; and (iv) establishment of a user-friendly web server. The eHCC-pred is freely accessible at http://www.dulab.com.cn/eHCC-pred/. Our approach, eHCC-pred, is anticipated to be robustly employed at the individual level for facilitating early HCC diagnosis in clinical practice, surpassing currently available state-of-the-art techniques.
Propyl gallate induces cell death in human pulmonary fibroblast through increasing reactive oxygen species levels and depleting glutathione
Authors: Park Woo Hyun
Published: March 2024

Propyl gallate (PG) exhibits an anti-growth effect on various cell types. The present study investigated the impact of PG on the levels of reactive oxygen species (ROS) and glutathione (GSH) in primary human pulmonary fibroblast (HPF) cells. Moreover, the effects of N-acetyl cysteine (NAC, an antioxidant), l-buthionine sulfoximine (BSO, a GSH synthesis inhibitor), and small interfering RNA (siRNAs) against various antioxidant genes on ROS and GSH levels and cell death were examined in PG-treated HPF cells. PG (100–800 μM) increased the levels of total ROS and O2·− at early time points of 30–180 min and 24 h, whereas PG (800–1600 μM) increased GSH-depleted cell number at 24 h and reduced GSH levels at 30–180 min. PG downregulated the activity of superoxide dismutase (SOD) and upregulated the activity of catalase in HPF cells. Treatment with 800 μM PG increased the number of apoptotic cells and cells that lost mitochondrial membrane potential (MMP; ΔΨm). NAC treatment attenuated HPF cell death and MMP (ΔΨm) loss induced by PG, accompanied by a decrease in GSH depletion, whereas BSO exacerbated the cell death and MMP (ΔΨm) loss without altering ROS and GSH depletion levels. Furthermore, siRNA against SOD1, SOD2, or catalase attenuated cell death in PG-treated HPF cells, whereas siRNA against GSH peroxidase enhanced cell death. In conclusion, PG induced cell death in HPF cells by increasing ROS levels and depleting GSH. NAC was found to decrease HPF cell death induced by PG, while BSO enhanced cell death. The findings shed light on how manipulating the antioxidant system influence the cytotoxic effects of PG in HPF cells.
Assessment of wound healing activity in diabetic mice treated with a novel therapeutic combination of selenium nanoparticles and platelets rich plasma
Authors: Karas Rania A. , Alexeree Shaimaa , Elsayed Hassan , Attia Yasser A.
Published: March 2024

Diabetic wound healing is sluggish, often ending in amputations. This study tested a novel, two-punch therapy in mice—Selenium nanoparticles (Se NPs) and platelet-rich plasma (PRP)—to boost healing. First, a mouse model of diabetes was created. Then, Se NPs were crafted for their impressive antioxidant and antimicrobial powers. PRP, packed with growth factors, was extracted from the mice's blood. Wound healing was tracked for 28 days through photos, scoring tools, and tissue analysis. Se NPs alone spurred healing, and PRP added extra fuel. Furthermore, when used in combination with PRP, the healing process was accelerated due to the higher concentration of growth factors in PRP. Notably, the combination of Se NPs and PRP exhibited a synergistic effect, significantly enhancing wound healing in diabetic mice. These findings hold promise for the treatment of diabetic wounds and have the potential to reduce the need for lower limb amputations associated with diabetic foot ulcers. The innovative combination therapy using Se NPs and PRP shows great potential in expediting the healing process and addressing the challenges of impaired wound healing in individuals with diabetes. This exciting finding suggests this therapy could change diabetic wound management, potentially saving limbs and improving lives.
Palaeoproteomics and microanalysis reveal techniques of production of animal-based metal threads in medieval textiles
Authors: Scibè Cristina , Eng-Wilmot Kira , Lam Thomas , Tosini Isetta , López Maria José González , Solazzo Caroline
Published: March 2024

Animal-based metal threads were largely used between the 10th and the fifteenth century, in European, Middle Eastern and Far Eastern textile productions for the decoration of textiles and cloths. They belong to a larger group of metal threads, used either as flat threads or wrapped around a fiber core, that were backed by an organic support (animal or paper). This study focuses on the medieval production of metal threads backed by an animal membrane (e.g. gut membrane), or skin. A total of 91 samples were collected from a corpus of 66 textile fragments belonging to 54 catalogued objects. The relevance and novelty of the present study is represented by the combination of proteomics, cross-section analysis, and scanning electron microscopy (SEM–EDS and SEM-µXRF). The diversity of materials and manufacturing techniques found within each typology of thread, respectively, membrane-based metal threads and skin-based metal threads, hinted at different production technologies. Membrane-based threads were found to be invariably made from cattle gut membrane, coated with gilt-silver leaves. A possible sheep glue adhesive was found in a few samples. Skin-based threads were made from either goat or sheep leather, coated with metal leaves or powder. Within the three different types of coatings identified (silver, gold and gilt-silver), gold coatings were the most represented. Goat leather threads were associated with an egg-white binder, while sturgeon glue was identified as adhesive in all sheep leather threads. Collagen glue from other species (cattle, sheep, horse) was occasionally found in mixed adhesives. In two textiles, the finding of human proteins indicates past contamination due to handling or use. The analytical results show coherence between the fabrication patterns of animal-based metal threads and their probable geographical areas of manufacture, indicating that the study of materials and techniques provide further criteria to classify and group textiles, and trace correlations between manufacturing centers within Eurasian territories.