bluuebunny/crossref_metadata_2025 huggingface dataset:
Time-Restricted G-Protein Signaling Pathways via GPR176, Gz, and RGS16 Set the Pace of the Master Circadian Clock in the Suprachiasmatic Nucleus
Authors: Nakagawa Shumpei , Nguyen Pham Khanh Tien , Shao Xinyan , Doi Masao
Published: July 2020

G-protein-coupled receptors (GPCRs) are an important source of drug targets with diverse therapeutic applications. However, there are still more than one hundred orphan GPCRs, whose ligands and functions remain unidentified. The suprachiasmatic nucleus (SCN) is the central circadian clock of the brain, directing daily rhythms in activity–rest behavior and physiology. Malfunction of the circadian clock has been linked to a wide variety of diseases, including sleep–wake disorders, obesity, diabetes, cancer, and hypertension, making the circadian clock an intriguing target for drug development. The orphan receptor GPR176 is an SCN-enriched orphan GPCR that sets the pace of the circadian clock. GPR176 undergoes asparagine (N)-linked glycosylation, a post-translational modification required for its proper cell-surface expression. Although its ligand remains unknown, this orphan receptor shows agonist-independent basal activity. GPR176 couples to the unique G-protein subclass Gz (or Gx) and participates in reducing cAMP production during the night. The regulator of G-protein signaling 16 (RGS16) is equally important for the regulation of circadian cAMP synthesis in the SCN. Genome-wide association studies, employing questionnaire-based evaluations of individual chronotypes, revealed loci near clock genes and in the regions containing RGS16 and ALG10B, a gene encoding an enzyme involved in protein N-glycosylation. Therefore, increasing evidence suggests that N-glycosylation of GPR176 and its downstream G-protein signal regulation may be involved in pathways characterizing human chronotypes. This review argues for the potential impact of focusing on GPCR signaling in the SCN for the purpose of fine-tuning the entire body clock.
A Novel ceRNA Regulatory Network Involving the Long Non-Coding Antisense RNA SPACA6P-AS, miR-125a and its mRNA Targets in Hepatocarcinoma Cells
Authors: Di Palo Armando , Siniscalchi Chiara , Mosca Nicola , Russo Aniello , Potenza Nicoletta
Published: July 2020

MicroRNAs (miRNA), and more recently long non-coding RNAs (lncRNA), are emerging as a driving force for hepatocellular carcinoma (HCC), one of the leading causes of cancer-related death. In this work, we investigated a possible RNA regulatory network involving two oncosuppressive miRNAs, miR-125a and let-7e, and a long non-coding antisense RNA, SPACA6P-AS (SP-AS), all transcribed from the same locus, with SP-AS in the opposite direction and thus carrying complementary sequences to the miRNAs. In vitro experiments validated the binding of the miRNAs to SP-AS. Then, the boosting of either the miRNAs or SP-AS levels demonstrated their reciprocal inhibition. In addition, overexpression of SP-AS resulted in a reduced silencing activity of miR-125a and let-7e toward their key oncogenic targets, i.e., Lin28b, MMP11, SIRT7, Zbtb7a, Cyclin D1, CDC25B, HMGA2, that resulted significantly upregulated. Finally, the analysis of 374 HCC samples in comparison to 50 normal liver tissues showed an upregulation of SP-AS and a reverse expression of miR-125a, not observed for let-7e; consistently, miR-125a oncogenic targets were upregulated. Overall, the data depict a novel competing endogenous RNA (ceRNA) network, ceRNET, whereby miR-125a can regulate the expression of SP-AS, which in turn regulates the miRNA by competing with the binding to the mRNA targets. We speculate that the unbalancing of any network component may contribute to hepatocarcinogenesis.
Methodological Proposal for the Study of Temporal and Spatial Dynamics during the Late Period between the Middle Ebro and the Pyrenees
Authors: Tobalina Pulido Leticia
Published: July 2020

The article I present here deals with the methodological approach carried out in my PhD in which I analyzed the spatial and temporal dynamics of late rural settlements during five centuries in the southern Pyrenees area, using geographic information systems, spatial databases, and descriptive statistics to establish models of space occupation and try to determine how these vary over the different centuries.
A Case for Security-Aware Design-Space Exploration of Embedded Systems
Authors: Pimentel Andy D.
Published: July 2020

As modern embedded systems are becoming more and more ubiquitous and interconnected, they attract a world-wide attention of attackers and the security aspect is more important than ever during the design of those systems. Moreover, given the ever-increasing complexity of the applications that run on these systems, it becomes increasingly difficult to meet all security criteria. While extra-functional design objectives such as performance and power/energy consumption are typically taken into account already during the very early stages of embedded systems design, system security is still mostly considered as an afterthought. That is, security is usually not regarded in the process of (early) design-space exploration of embedded systems, which is the critical process of multi-objective optimization that aims at optimizing the extra-functional behavior of a design. This position paper argues for the development of techniques for quantifying the ’degree of secureness’ of embedded system design instances such that these can be incorporated in a multi-objective optimization process. Such technology would allow for the optimization of security aspects of embedded systems during the earliest design phases as well as for studying the trade-offs between security and the other design objectives such as performance, power consumption and cost.
Shifts in Diatom Dominance Associated with Seasonal Changes in an Estuarine-Mangrove Phytoplankton Community
Authors: Hilaluddin Fareha , Yusoff Fatimah Md. , Toda Tatsuki
Published: July 2020

A study on seasonal phytoplankton abundance and composition in a mangrove estuary, Matang Mangrove Forest Reserve (MMFR), Malaysia, was carried out to determine the phytoplankton structure in this ecosystem, and to identify potential indicators of environmental changes. Phytoplankton samples were collected bimonthly from June 2010 to April 2011, to cover both dry (June to October) and wet (November to April) seasons, at four selected sampling sites along the river. Diatoms showed the highest number of species (50 species) from a total of 85 phytoplankton species from 76 genera. Diatoms contributed more than 90% of the total phytoplankton abundance during the dry season (southwest monsoon) and less than 70% during the wet season (northeast monsoon) as dinoflagellates became more abundant during the rainy season. Two diatoms were recorded as dominant species throughout the sampling period; Cyclotella sp. and Skeletonema costatum. Cyclotella sp. formed the most abundant species (62% of total phytoplankton) during the dry period characterized by low nutrients and relatively low turbidity. Skeletonema costatum contributed 93% of the total phytoplankton in October, which marked the end of the dry season and the beginning of the wet season, characterized by strong winds and high waves leading to the upwelling of the water column. Massive blooms of Skeletonema costatum occurred during the upwelling when total nitrogen (TN) and total phosphorus (TP) concentrations were highest (p < 0.05) throughout the year. The abundance of diatom species during the wet season was more evenly distributed, with most diatom species contributing less than 12% of the total phytoplankton. Autotrophic producers such as diatoms were limited by high turbidity during the northeast monsoon when the rainfall was high. During the wet season, Cyclotella and Skeletonema costatum only contributed 9% and 5% of the total phytoplankton, respectively, as dinoflagellates had more competitive advantage in turbid waters. This study illustrates that some diatom species such as Cyclotella sp. and Skeletonema costatum could be used as indicators of the environmental changes in marine waters.
Cytotoxicity and Gene Expression Changes of a Novel Homeopathic Antiseptic Oral Rinse in Comparison to Chlorhexidine in Gingival Fibroblasts
Authors: Fujioka-Kobayashi Masako , Schaller Benoit , Pikos Michael A. , Sculean Anton , Miron Richard J.
Published: July 2020

Most available antiseptic solutions available today have strong antibacterial effects, however most also possess major cytotoxic effects on human gingival tissues. The VEGA Oral Care Recovery Kit (StellaLife), previously evaluated in clinical studies, consists of 16 active ingredients that are monographed in the Homeopathic Pharmacopeia of United States (HPUS) and recognized for their accelerated healing properties (reduction in post-op pain). The aim of this study was to compare VEGA to chlorhexidine (CHX) in vitro on gingival fibroblast viability, survival at various concentrations, migration assay, proliferation activity, expression of both regenerative growth factors as well as inflammatory markers, and collagen synthesis. A 10-fold dilution of standard CHX (0.02%) led to cell death, whereas cell viability was significantly better in the VEGA group for all tested parameters. Furthermore, VEGA also induced significantly greater fibroblast migration and proliferation. CHX negatively impacted the cellular inflammatory response of gingival fibroblasts, and also led to a reduction in collagen synthesis (50% decrease). Findings from the present study provide support from basic laboratory experiments that validate the previous clinical studies supporting the use of the VEGA oral rinse on its superior biocompatibility and wound healing properties when compared to CHX.
Study on the Compressive Properties of Magnesium Phosphate Cement Mixing with Eco-Friendly Coir Fiber Considering Fiber Length
Authors: Jiang Zuqian , Zhang Liwen , Geng Tao , Lai Yushan , Zheng Weile , Huang Min
Published: July 2020

Coir fiber (CF), an eco-friendly and renewable natural fiber, was introduced into magnesium phosphate cement (MPC) mortar to improve its crack resistance. A total of 21 specimens were employed to investigate the failure pattern, compressive strength, stress–strain curve, and energy absorption of MPC with varying CF lengths (0, 5, 10, 15, 20, 25, and 30 mm) after a curing period of 28 days through a static compressive test. The results demonstrated that compressive strength, elastic modulus, and secant modulus decreased with the increase in CF length. However, energy absorption presented a convex curve, which increased to the maximum value (77.0% relative to the value of the specimen without CF) with a CF length of 20 mm and then declined. A series of modern micro-tests were then carried out to analyze the microstructure and composition of specimens to explain the properties microscopically.
Influence of e-Beam Irradiation on the Physicochemical Properties of Poly(polyol Succinate-co-Butylene Succinate) Ester Elastomers
Authors: Piątek-Hnat Marta , Bomba Kuba , Pęksiński Jakub , Kozłowska Agnieszka , Sośnicki Jacek G. , Idzik Tomasz J. , Piwowarska Danuta , Janik Jolanta
Published: July 2020

The purpose of this research was synthesis and electron beam modification of novel ester elastomers consisting of sugar alcohol–succinic acid block and butylene glycol–succinic acid block. Four different alditols were used in the synthesis—sorbitol, erythritol, xylitol, and glycerol. The materials were irradiated with doses of 50, 100, and 150 kGy in order to determine which dose is the most beneficial. As expected, irradiation of the materials has led to the cross-link density becoming higher and improvement of the mechanical properties. Additionally, the materials were also sterilized in the process. The great advantage of elastomers described in the paper is the fact that they do not need chemical cross-linking agents or sensitizers in order to undergo radiation modification. The following tests were performed on cross-linked poly(polyol succinate-co-butylene succinate) elastomers: quasi-static tensile test, determination of cross-link density, differential scanning calorimetry (DSC), dynamic thermomechanical analysis (DMTA), wettability (water contact angle), and Fourier transform infrared spectroscopy (FTIR). In order to confirm successful synthesis, prepolymers were analyzed by nuclear magnetic resonance spectroscopy (1H NMR and 13C NMR).
Effectiveness of 6-Week Nordic Walking Training on Functional Performance, Gait Quality, and Quality of Life in Parkinson’s Disease
Authors: Szefler-Derela Justyna , Arkuszewski Michal , Knapik Andrzej , Wasiuk-Zowada Dagmara , Gorzkowska Agnieszka , Krzystanek Ewa
Published: July 2020

Background and objectives: Motor rehabilitation improves physical mobility and quality of life in Parkinson’s disease (PD). As specialized rehabilitation is expensive and resource-consuming, there is a need for simpler, cost-effective methods. The purpose of the study was to determine whether Nordic Walking (NW) training may support the management of motor disability in PD. Materials and Methods: Forty patients (median age 64.0 years, range 50–75 years) with idiopathic PD, Hoehn and Yahr stages II–III, were randomly assigned to NW or standard rehabilitation (SR) programs, comprising twelve rehabilitation sessions conducted bi-weekly throughout the 6-week study period. Results: Median Unified Parkinson’s Disease Rating Scale part III scores were significantly reduced with NW, by 8.5, and with SR, by 6.0 points (both p < 0.001), with significantly greater improvement with NW than with SR (p = 0.047). Gait quality and balance control, measured using the Dynamic Gait Index, improved with NW by a median of 8.0 and with SR by 5.5 points (both p < 0.001), with slightly greater improvement with NW, compared to the SR group (p = 0.064). Quality of life, assessed using the Parkinson’s Disease Questionnaire (PDQ-39), improved with NW by a median of 15 and with SR by 12 points, p = 0.001 and p = 0.008, respectively. Conclusions: The 6-week Nordic Walking program improves functional performance, quality of gait, and quality of life in patients with PD and has comparable effectiveness to standard rehabilitation.
Progression to Severe Hypernatremia in Hospitalized General Medicine Inpatients: An Observational Study of Hospital-Acquired Hypernatremia
Authors: Ranjan Ramessh , Lo Stacey C.-Y. , Ly Stephanie , Krishnananthan Visakan , Lim Andy K.H.
Published: July 2020

Background and objectives: Hypernatremia can be community or hospital-acquired, and there may be specific factors unique to the hospital environment, such as intravenous fluid treatment, which contribute to hypernatremia. The aim of this study was to determine the factors associated with the progression from moderate to severe hospital-acquired hypernatremia among patients admitted under general medicine. Materials and Methods: In this retrospective, single-center cohort study (2012 to 2017), we used ICD-10 coding and medical records to identify adult patients who developed moderate hypernatremia and followed them for progression to severe hypernatremia. We profiled the serum biochemistry and the volume and composition of prescribed intravenous fluids. We applied logistic regression to determine the factors associated with the progression to severe hypernatremia, using the patients with moderate hypernatremia as reference. Results: Of the 180 medical inpatients (median age of 81 years) with moderate hospital-acquired hypernatremia, 9.4% progressed to severe hypernatremia. Normal saline comprised 76% of intravenous fluid volume administered prior to onset of moderate hypernatremia. After the onset, 38% of fluid volume prescribed remained normal saline. The factors independently associated with progression to severe hypernatremia included chronic kidney disease stage (odds ratio 2.38, 95% CI: 1.26–4.50, P = 0.008) and serum creatinine increase (per 10 µmol/L, OR 1.29, 95% CI: 1.07–1.57, P = 0.009). Conclusions: Patients with chronic kidney disease and acute kidney injury may have an increased risk of severe hospital-acquired hypernatremia.
Overview of Ultrasound Detection Technologies for Photoacoustic Imaging
Authors: Manwar Rayyan , Kratkiewicz Karl , Avanaki Kamran
Published: July 2020

Ultrasound detection is one of the major components of photoacoustic imaging systems. Advancement in ultrasound transducer technology has a significant impact on the translation of photoacoustic imaging to the clinic. Here, we present an overview on various ultrasound transducer technologies including conventional piezoelectric and micromachined transducers, as well as optical ultrasound detection technology. We explain the core components of each technology, their working principle, and describe their manufacturing process. We then quantitatively compare their performance when they are used in the receive mode of a photoacoustic imaging system.
Thermal and Photo Sensing Capabilities of Mono- and Few-Layer Thick Transition Metal Dichalcogenides
Authors: Voshell Andrew , Terrones Mauricio , Rana Mukti
Published: July 2020

Two-dimensional (2D) materials have shown promise in various optical and electrical applications. Among these materials, semiconducting transition metal dichalcogenides (TMDs) have been heavily studied recently for their photodetection and thermoelectric properties. The recent progress in fabrication, defect engineering, doping, and heterostructure design has shown vast improvements in response time and sensitivity, which can be applied to both contact-based (thermocouple), and non-contact (photodetector) thermal sensing applications. These improvements have allowed the possibility of cost-effective and tunable thermal sensors for novel applications, such as broadband photodetectors, ultrafast detectors, and high thermoelectric figures of merit. In this review, we summarize the properties arisen in works that focus on the respective qualities of TMD-based photodetectors and thermocouples, with a focus on their optical, electrical, and thermoelectric capabilities for using them in sensing and detection.
Tick-Borne Encephalitis Virus and Its European Distribution in Ticks and Endothermic Mammals
Authors: Walter Melanie , Vogelgesang Janna R. , Rubel Franz , Brugger Katharina
Published: July 2020

Tick-borne encephalitis (TBE) is the most common viral tick-borne disease in Europe causing thousands of human infections every year. Available risk maps in Europe are solely based on human incidences, but often underestimate areas with TBE virus circulation as shown by several autochthonous cases detected outside known risk areas. A dataset of more than 1300 georeferenced TBE virus detections in ticks and mammals except for humans was compiled and used to estimate the probability of TBE virus presence in Europe. For this, a random forests model was implemented using temperature- and precipitation-dependent bioclimatic variables of the WorldClim dataset, altitude, as well as land cover of the ESA GlobCover dataset. The highest probabilities of TBE virus presence were identified in Central Europe, in the south of the Nordic countries, and in the Baltic countries. The model performance was evaluated by an out-of-bag error (OOB) of 0.174 and a high area under the curve value (AUC) of 0.905. The TBE virus presence maps may subsequently be used to estimate the risk of TBE virus infections in humans and can support decision-makers to identify TBE risk areas and to encourage people to take appropriate actions against tick bites and TBE virus infections.
Simultaneous Quantification of Forsterite Content and Minor–Trace Elements in Olivine by LA–ICP–MS and Geological Applications in Emeishan Large Igneous Province
Authors: Wu Shitou , Wu Yadong , Yang Yueheng , Wang Hao , Huang Chao , Xie Liewen , Yang Jinhui
Published: July 2020

Olivine forsterite contents [Fo = 100 × Mg/(Mg + Fe) in mol%] and minor–trace element concentrations can aid our understanding of the Earth’s mantle. Traditionally, these data are obtained by electron probe microanalysis for Fo contents and minor elements, and then by laser ablation–inductively coupled plasma–mass spectrometry (LA–ICP–MS) for trace elements. In this study, we demonstrate that LA–ICP–MS, with a simplified 100% quantification approach, allows the calculation of Fo contents simultaneously with minor–trace elements. The approach proceeds as follows: (1) calculation of Fo contents from measured Fe/Mg ratios; (2) according to the olivine stoichiometric formula [(Mg, Fe)2SiO4] and known Fo contents, contents of Mg, Fe and Si can be computed, which are used as internal standards for minor–trace element quantification. The Fo content of the MongOLSh 11-2 olivine reference material is 89.55 ± 0.15 (2 s; N = 120), which agrees with the recommended values of 89.53 ± 0.05 (2 s). For minor–trace elements, the results matched well with the recommended values, apart from P and Zn data. This technique was applied to olivine phenocrysts in the Lijiang picrites from the Emeishan large igneous province. The olivine compositions suggest that the Lijiang picrites have a peridotitic mantle source.
Current Trends in Nanomaterials for Metal Oxide-Based Conductometric Gas Sensors: Advantages and Limitations. Part 1: 1D and 2D Nanostructures
Authors: Korotcenkov Ghenadii
Published: July 2020

This article discusses the main uses of 1D and 2D nanomaterials in the development of conductometric gas sensors based on metal oxides. It is shown that, along with the advantages of these materials, which can improve the parameters of gas sensors, there are a number of disadvantages that significantly limit their use in the development of devices designed for the sensor market.
Anti-Diabetic Effects of Allulose in Diet-Induced Obese Mice via Regulation of mRNA Expression and Alteration of the Microbiome Composition
Authors: Han Youngji , Kwon Eun-Young , Choi Myung-Sook
Published: July 2020

Allulose has been reported to serve as an anti-obesity and anti-diabetic food component; however, its molecular mechanism is not yet completely understood. This study aims to elucidate the mechanisms of action for allulose in obesity-induced type 2 diabetes mellitus (T2DM), by analyzing the transcriptional and microbial populations of diet-induced obese mice. Thirty-six C57BL/6J mice were divided into four groups, fed with a normal diet (ND), a high-fat diet (HFD), a HFD supplemented with 5% erythritol, or a HFD supplemented with 5% allulose for 16 weeks, in a pair-fed manner. The allulose supplement reduced obesity and comorbidities, including inflammation and hepatic steatosis, and changed the microbial community in HFD-induced obese mice. Allulose attenuated obesity-mediated inflammation, by downregulating mRNA levels of inflammatory response components in the liver, leads to decreased plasma pro-inflammatory marker levels. Allulose suppressed glucose and lipid metabolism-regulating enzyme activities, ameliorating hepatic steatosis and improving dyslipidemia. Allulose improved fasting blood glucose (FBG), plasma glucose, homeostatic model assessment of insulin resistance (HOMA-IR), and the area under the curve (AUC) for the intraperitoneal glucose tolerance test (IPGTT), as well as hepatic lipid levels. Our findings suggested that allulose reduced HFD-induced obesity and improved T2DM by altering mRNA expression and the microbiome community.
Adherence to the Mediterranean Lifestyle and Desired Body Weight Loss in a Mediterranean Adult Population with Overweight: A PREDIMED-Plus Study
Authors: Bouzas Cristina , Bibiloni Maria del Mar , Julibert Alicia , Ruiz-Canela Miguel , Salas-Salvadó Jordi , Corella Dolores , Zomeño Maria Dolors , Romaguera Dora , Vioque Jesús , Alonso-Gómez Ángel M. , Wärnberg Julia , Martínez J. Alfredo , Serra-Majem Luís , Estruch Ramon , Tinahones Francisco J. , Lapetra José , Pintó Xavier , García Ríos Antonio , Bueno-Cavanillas Aurora , Gaforio José J. , Matía-Martín Pilar , Daimiel Lidia , Martín-Sánchez Vicente , Vidal Josep , Vázquez Clotilde , Ros Emilio , Fernandez-Lázaro Cesar Ignacio , Becerra-Tomás Nerea , Gimenez-Alba Ignacio Manuel , Muñoz Julia , Morey Marga , Oncina-Canovas Alejandro , Tojal-Sierra Lucas , Pérez-López Jéssica , Abete Itziar , Casañas-Quintana Tamara , Castro-Barquero Sara , Bernal-López M. Rosa , Santos-Lozano José Manuel , Galera Ana , Angullo-Martinez Escarlata , Basterra-Gortari F. Javier , Basora Josep , Saiz Carmen , Castañer Olga , Martín Marian , Notario-Barandiarán Leyre , Belló-Mora María C. , Sayón-Orea Carmen , García-Gavilán Jesús , Goday Albert , Tur Josep A.
Published: July 2020

Background. Body weight dissatisfaction is a hindrance to following a healthy lifestyle and it has been associated with weight concerns. Objectives. The aim of this study was to assess the association between the adherence to the Mediterranean lifestyle (diet and exercise) and the desired body weight loss in an adult Mediterranean population with overweight. Methods. Cross-sectional analysis in 6355 participants (3268 men; 3087 women) with metabolic syndrome and BMI (Body mass index) between 27.0 and 40.0 kg/m2 (55–75 years old) from the PREDIMED-Plus trial. Desired weight loss was the percentage of weight that participants wished to lose. It was categorized into four cut-offs of this percentage (Q1: <10%, n = 1495; Q2: 10–15%, n = 1804; Q3: <15–20%, n = 1470; Q4: ≥20%, n = 1589). Diet was assessed using a validated food frequency questionnaire and a 17-item Mediterranean diet questionnaire. Physical activity was assessed by the validated Minnesota-REGICOR and the validated Spanish version of the Nurses’ Health Study questionnaire. Results. Participants reporting higher percentages of desired weight loss (Q3 and Q4) were younger, had higher real and perceived BMI and were more likely to have abdominal obesity. Desired weight loss correlated inversely to physical activity (Q1: 2106 MET min/week; Q4: 1585 MET min/week. p < 0.001) and adherence to Mediterranean diet (Q1: 8.7; Q4: 8.3. p < 0.001). Conclusions. In older Mediterranean individuals with weight excess, desired weight loss was inversely associated with Mediterranean lifestyle adherence. Deeply rooted aspects of the MedDiet remained similar across groups. Longitudinal research is advised to be able to establish causality.
Addendum: Uberti, F.; et al. Study of Magnesium Formulations on Intestinal Cells to Influence Myometrium Cell Relaxation. Nutrients 2020, 12, 573
Authors: Uberti Francesca , Morsanuto Vera , Ruga Sara , Galla Rebecca , Farghali Mahitab , Notte Felice , Bozzo Chiarella , Magnani Corrado , Nardone Antonio , Molinari Claudio
Published: July 2020

The authors would like to publish this addendum to their recent publication [...]
Efficacy of a High-Iron Dietary Intervention in Women with Celiac Disease and Iron Deficiency without Anemia: A Clinical Trial
Authors: Scricciolo Alice , Elli Luca , Doneda Luisa , Bascunan Karla A , Branchi Federica , Ferretti Francesca , Vecchi Maurizio , Roncoroni Leda
Published: July 2020

Background and Aim. Iron deficiency without anemia (IDWA) is a common finding in celiac disease (CD) and can also persist in case of good compliance and clinical response to a strict gluten-free diet (GFD). This scenario usually presents in CD women of child-bearing age in whom the imbalance between menstrual iron loss and inadequate iron intake from their diet plays the major role. A recommended approach to this condition is yet to be established. This study aimed to compare, in this subset of patients, the efficacy of a dietary approach consisting of an iron-rich diet against the traditional pharmacological oral-replacement therapy. Material and Methods. Between February and December 2016, consecutive CD female patients of child-bearing age as referred to our outpatient center with evidence of IDWA (ferritin <15 ng/mL or 15–20 ng/L with transferrin saturation <15%) were enrolled. After the completion of a 7-day weighed food intake recording to assess the usual iron dietary intake, the patients were randomized in two arms to receive a 12-week iron-rich diet (iron intake >20 mg/die) versus oral iron supplementation with ferrous sulfate (FS) (105 mg/day). Blood tests and dietary assessments were repeated at the end of treatment. The degree of compliance and tolerability to the treatments were assessed every month by means of specific questionnaires and symptoms evaluation. Results. A total of 22 women were enrolled and divided in the diet group (n = 10, age 37 ± 8 years) and in the FS group (n = 12, age 38 ± 10 years). The food intake records demonstrated an inadequate daily intake of iron in all the enrolled subjects. At the end of the treatments, ferritin levels were higher in the FS group (8.5 (5) versus 34 (30.8), p = 0.002). Compliance and tolerability were similar in both treatment groups (89% versus 87%, p = ns). Conclusions. These findings did not support any equivalent efficacy of an iron-rich diet compared to a FS supplementation in non-anemic iron-deficient women affected by CD. However, the diet appeared a well-tolerated approach, and adequate dietary instructions could effectively increase the daily iron consumption, suggesting a role in the long-term management of IDWA, especially in patients who do not tolerate pharmacological supplementation.
The Effect of Dietary Inorganic Nitrate Supplementation on Cardiac Function during Submaximal Exercise in Men with Heart Failure with Reduced Ejection Fraction (HFrEF): A Pilot Study
Authors: Woessner Mary N. , Levinger Itamar , Allen Jason D. , McIlvenna Luke C. , Neil Christopher
Published: July 2020

Heart failure with reduced ejection fraction (HFrEF) is a common end point for patients with coronary artery disease and it is characterized by exercise intolerance due, in part, to a reduction in cardiac output. Nitric oxide (NO) plays a vital role in cardiac function and patients with HFrEF have been identified as having reduced vascular NO. This pilot study aimed to investigate if nitrate supplementation could improve cardiac measures during acute, submaximal exercise. Five male participants (61 ± 3 years) with HFrEF (EF 32 ± 2.2%) completed this pilot study. All participants supplemented with inorganic nitrate (beetroot juice) or a nitrate-depleted placebo for ~13 days prior to testing. Participants completed a three-stage submaximal exercise protocol on a recumbent cycle ergometer with simultaneous echocardiography for calculation of cardiac output (Q), stroke volume (SV), and total peripheral resistance (TPR). Heart rate and blood pressure were measured at rest and during each stage. Both plasma nitrate (mean = ~1028%, p = 0.004) and nitrite (mean = ~109%, p = 0.01) increased following supplementation. There were no differences between interventions at rest, but the percent change in SV and Q from rest to stage two and stage three of exercise was higher following nitrate supplementation (all p > 0.05, ES > 0.8). Both interventions showed decreases in TPR during exercise, but the percent reduction TPR in stages two and three was greater following nitrate supplementation (p = 0.09, ES = 0.98 and p = 0.14, ES = 0.82, respectively). There were clinically relevant increases in cardiac function during exercise following supplementation with nitrate. The findings from this pilot study warrant further investigation in larger clinical trials.
Pichinde Virus Infection of Outbred Hartley Guinea Pigs as a Surrogate Animal Model for Human Lassa Fever: Histopathological and Immunohistochemical Analyses
Authors: Shieh Wun-Ju , Lan Shuiyun , Zaki Sherif R. , Ly Hinh , Liang Yuying
Published: July 2020

Lassa virus (LASV) is a mammarenavirus (arenavirus) that causes zoonotic infection in humans that can lead to fatal hemorrhagic Lassa fever (LF) disease. Currently, there are no FDA-approved vaccines or therapeutics against LASV. Development of treatments against LF and other related arenavirus-induced hemorrhagic fevers (AHFs) requires relevant animal models that can recapitulate clinical and pathological features of AHF diseases in humans. Laboratory mice are generally resistant to LASV infection, and non-human primates, while being a good animal model for LF, are limited by their high cost. Here, we describe a small, affordable, and convenient animal model that is based on outbred Hartley guinea pigs infected with Pichinde virus (PICV), a mammarenavirus that is non-pathogenic in humans, for use as a surrogate model of human LF. We conducted a detailed analysis of tissue histopathology and immunohistochemical analysis of different organs of outbred Hartley guinea pigs infected with different PICV strains that show differential disease phenotypes and pathologies. Comparing to infection with the avirulent PICV strain (P2 or rP2), animals infected with the virulent strain (P18 or rP18) show extensive pathological changes in different organs that sustain high levels of virus replication. The similarity of tissue pathology and viral antigen distribution between the virulent PICV–guinea pig model and lethal human LASV infection supports a role of this small animal model as a surrogate model of studying human LF in order to understand its pathogenesis and for evaluating potential preventative and therapeutic options against AHFs.
Becoming a Shaman: Narratives of Apprenticeship and Initiation in Contemporary Shamanism
Authors: Ivanescu Carolina , Berentzen Sterre
Published: July 2020

This article, based on an open-question survey completed in 2018, engages with McAdams and Manczak’s approaches to life stories (2015) and Mayer’s ten elements of the shaman myth (2008) to explore the way contemporary people based in the UK, who define themselves as shamans, talk about their becoming a shaman. Individual narratives point out the intricate meeting points between different shamanic traditions and the importance of continuous innovation. They highlight the complex network of human and beyond-human authority and problematize the place, meaning and agency of the self. Contemporary shamanism is a widespread, manifold and multifaceted phenomenon, which we argue is not as different from traditional forms of shamanism as some studies suggest.
Words and Pictures: Rāmāyaṇa Traditions and the Art of Ekphrasis
Authors: Kaligotla Subhashini
Published: July 2020

This article examines two ambitious enactments of the Rama story or Rāmāyaṇa, side by side: the 17th-century painted Mewar Rāmāyaṇa and Vālmīki’s epic poem (ca. 750–500 BCE). Through a formal analysis of two crucial episodes of the tale, it highlights the creative tactics of each medium and stresses their separate aesthetic interests and autonomy. While A. K. Ramanujan’s notion of the “telling” has been immensely influential in South Asian studies to theorize the Rāmāyaṇa’s multiplicity, that concept tends to privilege speech-based embodiments. I propose, by contrast, that ekphrasis may be a more broadly applicable lens. Understanding ekphrasis as an enactment of the Rama story in any medium or in interart media, I advocate for considering poetry and painting on an equal plane as opposed to the standard view of the Mewar paintings as visual translations of linguistic phenomena. Ekphrasis, as a gateway to the maker’s creative process and preoccupations, is central to the paper’s argument, as is the role receivers play in the act of Rāmāyaṇa making.
Commemorating the Nameless Wives of the Bible: Midrashic Poems by Contemporary American-Jewish Women
Authors: Koplowitz-Breier Anat
Published: July 2020

A proper name individualizes a person, the lack of it making him or her less noticeable. This insight is apt in regard to the nameless women in the Hebrew Bible, a resolutely androcentric work. As Judaism traditionally barred women from studying, many Jewish feminists have sought access to the Jewish canon. Much of American-Jewish women’s poetry can thus be viewed as belonging to the midrashic-poetry tradition, attempting to vivify the biblical women by “revisioning” the Bible. This article examines two nameless wives who, although barely noted in the biblical text, play a significant role in their husbands’ stories—Mrs. Noah and Mrs. Job. Although numerous exegetes have noted them across history, few have delved into their emotions and characters. Exploration of the way in which contemporary Jewish-American poets treat these women and connect them to their own world(s) is thus of great interest to both modern and biblical scholars. Herein I focus on five poets: Elaine Rose Glickman (“Parashat Noach”), Barbara D. Holender (“Noah’s Wife,” and “Job’s Wife”), Oriana Ivy (“Mrs. Noah,” and “Job’s Wife”), Shirley Kaufman (“Job’s Wife”), and Sherri Waas Shunfenthal (“Noah’s Wife Speaks,” “The Animals are our Friends,” “Time,” and “Arc of Peace”).
Sentinel-2 Data for Land Cover/Use Mapping: A Review
Authors: Phiri Darius , Simwanda Matamyo , Salekin Serajis , Nyirenda Vincent , Murayama Yuji , Ranagalage Manjula
Published: July 2020

The advancement in satellite remote sensing technology has revolutionised the approaches to monitoring the Earth’s surface. The development of the Copernicus Programme by the European Space Agency (ESA) and the European Union (EU) has contributed to the effective monitoring of the Earth’s surface by producing the Sentinel-2 multispectral products. Sentinel-2 satellites are the second constellation of the ESA Sentinel missions and carry onboard multispectral scanners. The primary objective of the Sentinel-2 mission is to provide high resolution satellite data for land cover/use monitoring, climate change and disaster monitoring, as well as complementing the other satellite missions such as Landsat. Since the launch of Sentinel-2 multispectral instruments in 2015, there have been many studies on land cover/use classification which use Sentinel-2 images. However, no review studies have been dedicated to the application of ESA Sentinel-2 land cover/use monitoring. Therefore, this review focuses on two aspects: (1) assessing the contribution of ESA Sentinel-2 to land cover/use classification, and (2) exploring the performance of Sentinel-2 data in different applications (e.g., forest, urban area and natural hazard monitoring). The present review shows that Sentinel-2 has a positive impact on land cover/use monitoring, specifically in monitoring of crop, forests, urban areas, and water resources. The contemporary high adoption and application of Sentinel-2 can be attributed to the higher spatial resolution (10 m) than other medium spatial resolution images, the high temporal resolution of 5 days and the availability of the red-edge bands with multiple applications. The ability to integrate Sentinel-2 data with other remotely sensed data, as part of data analysis, improves the overall accuracy (OA) when working with Sentinel-2 images. The free access policy drives the increasing use of Sentinel-2 data, especially in developing countries where financial resources for the acquisition of remotely sensed data are limited. The literature also shows that the use of Sentinel-2 data produces high accuracies (>80%) with machine-learning classifiers such as support vector machine (SVM) and Random forest (RF). However, other classifiers such as maximum likelihood analysis are also common. Although Sentinel-2 offers many opportunities for land cover/use classification, there are challenges which include mismatching with Landsat OLI-8 data, a lack of thermal bands, and the differences in spatial resolution among the bands of Sentinel-2. Sentinel-2 data show promise and have the potential to contribute significantly towards land cover/use monitoring.
OPEN: A New Estimation of Global Ocean Heat Content for Upper 2000 Meters from Remote Sensing Data
Authors: Su Hua , Zhang Haojie , Geng Xupu , Qin Tian , Lu Wenfang , Yan Xiao-Hai
Published: July 2020

Retrieving information concerning the interior of the ocean using satellite remote sensing data has a major impact on studies of ocean dynamic and climate changes; however, the lack of information within the ocean limits such studies about the global ocean. In this paper, an artificial neural network, combined with satellite data and gridded Argo product, is used to estimate the ocean heat content (OHC) anomalies over four different depths down to 2000 m covering the near-global ocean, excluding the polar regions. Our method allows for the temporal hindcast of the OHC to other periods beyond the 2005–2018 training period. By applying an ensemble technique, the hindcasting uncertainty could also be estimated by using different 9-year periods for training and then calculating the standard deviation across six ensemble members. This new OHC product is called the Ocean Projection and Extension neural Network (OPEN) product. The accuracy of the product is accessed using the coefficient of determination (R2) and the relative root-mean-square error (RRMSE). The feature combinations and network architecture are optimized via a series of experiments. Overall, intercomparison with several routinely analyzed OHC products shows that the OPEN OHC has an R2 larger than 0.95 and an RRMSE of <0.20 and presents notably accurate trends and variabilities. The OPEN product can therefore provide a valuable complement for studies of global climate changes.
Security Aspects in Smart Meters: Analysis and Prevention
Authors: Díaz Redondo Rebeca P. , Fernández-Vilas Ana , Fernández dos Reis Gabriel
Published: July 2020

Smart meters are of the basic elements in the so-called Smart Grid. These devices, connected to the Internet, keep bidirectional communication with other devices in the Smart Grid structure to allow remote readings and maintenance. As any other device connected to a network, smart meters become vulnerable to attacks with different purposes, like stealing data or altering readings. Nowadays, it is becoming more and more popular to buy and plug-and-play smart meters, additionally to those installed by the energy providers, to directly monitor the energy consumption at home. This option inherently entails security risks that are under the responsibility of householders. In this paper, we focus on an open solution based on Smartpi 2.0 devices with two purposes. On the one hand, we propose a network configuration and different data flows to exchange data (energy readings) in the home. These flows are designed to support collaborative among the devices in order to prevent external attacks and attempts of corrupting the data. On the other hand, we check the vulnerability by performing two kind of attacks (denial of service and stealing and changing data by using a malware). We conclude that, as expected, these devices are vulnerable to these attacks, but we provide mechanisms to detect both of them and to solve, by applying cooperation techniques.
A Siamese Network-Based Non-Contact Measurement Method for Railway Catenary Uplift Trained in a Free Vibration Test
Authors: Duan Fuchuan , Liu Zhigang , Zhai Donghai , Rønnquist Anders
Published: July 2020

The vibration of the catenary that is initiated by the passing pantograph has a direct influence on the pantograph–catenary contact performance. Monitoring the dynamic uplift of the catenary can help inspectors to evaluate the railway operation conditions and investigate the mechanism of pantograph–catenary interaction further. In this paper, a non-contact measurement method based on the deep leaning method is proposed to monitor the real-time vibration of the catenary. The field test for the catenary free vibration is designed to validate the method’s performance. The measurement method is developed based on the fully convolutional Siamese neural network, and the contact wire is taken as the tracking target. To reduce the recognition errors caused by the changes in the shape and grayscale of the moving contact wire in images, the class-agnostic binary segmentation mask is adopted. A developed down-sampling block is used in the neural network to reduce the image feature loss, which effectively enhances the recognition effect for the catenary vibration under variable lighting conditions. To validate the performance of the proposed measurement method, a series of field tests of catenary free vibration were conducted under various lighting conditions and different excitations, and the recognition results were compared with traditional target tracking methods. The results show that the proposed method performs well for catenary vibration identification in the field test. Additionally, the uplift data extracted from the identified images agree with the numerical results, and also help to further investigate the wave propagation and damping characteristics in the catenary structure.
A Bayesian Adaptive Design in Cancer Phase I Trials Using Dose Combinations with Ordinal Toxicity Grades
Authors: Diniz Márcio A. , Kim Sungjin , Tighiouart Mourad
Published: July 2020

We propose a Bayesian adaptive design for early phase drug combination cancer trials incorporating ordinal grade of toxicities. Parametric models are used to describe the relationship between the dose combinations and the probabilities of the ordinal toxicities under the proportional odds assumption. Trial design proceeds by treating cohorts of two patients simultaneously receiving different dose combinations. Specifically, at each stage of the trial, we seek the dose of one agent by minimizing the Bayes risk with respect to a loss function given the current dose of the other agent. We consider two types of loss functions corresponding to the Continual Reassessment Method (CRM) and Escalation with Overdose Control (EWOC). At the end of the trial, we estimate the MTD curve as a function of Bayes estimates of the model parameters. We evaluate design operating characteristics in terms of safety of the trial and percent of dose recommendation at dose combination neighborhoods around the true MTD by comparing this design to the one that uses a binary indicator of DLT. The methodology is further adapted to the case of a pre-specified discrete set of dose combinations.
Assessment of the Resilience of a Tartary Buckwheat (Fagopyrum tataricum) Cultivation System in Meigu, Southwest China
Authors: Song Yingjie , Jarvis Devra I. , Bai Keyu , Feng Jinchao , Long Chunlin
Published: July 2020

Recent socioeconomic development, increased transport and new agricultural technology are endangering the survival of traditional agriculture and the Yi people’s traditional knowledge of cultivating Tartary buckwheat. The cultural heritage of Tartary buckwheat cultivation among the Yi communities needs to be investigated and protected before its loss. The main objectives of this study are to document the Tartary buckwheat cultivation system, to analyze the agroecosystem networks that support the current system, and to measure the resilience of the ecological, agricultural and social systems using relevant indicators. The Tartary buckwheat cultivation system in Meigu County uses a rotation system, in which various crops are planted alternatively (e.g., Tartary buckwheat, green manure and potato/corn), utilizing bunch planting and furrow drilling technology. Tartary buckwheat has an important position in the major festival activities among the Yi people’s communities. Network analysis on the current agricultural system, ecosystem and social system indicated that the system was stable. The mean score of ecological, agricultural and social stability were 2.50, 2.85 and 2.53, respectively, indicating moderately stability. In contrast, socio-ecological production landscapes and seascapes (SEPLS) resilience indicators in Meigu performed only moderately, with a score of 2.63. The assessment of the resilience of the Tartary buckwheat cultivation system can provide some guidance for policy makers to strengthen biodiversity conservation, sustainable agricultural production and livelihood development (e.g., land use, responding to extreme environmental stresses and improving education levels).