bluuebunny/crossref_metadata_2025 huggingface dataset:
The Suitability of Potential Organ Donors Using Real Case-Scenarios; Do we Need to Create a “Donor Board” Process for Donors Perceived as Unlikely Suitable?
Authors: Marsolais Pierre , Larouche Gabrielle , Lagacé Anne-Marie , Williams Virginie , Serri Karim , Bernard Francis , Rico Philippe , Frenette Anne Julie , Williamson David , Albert Martin , Charbonney Emmanuel
Published: May 2022

Introduction: Despite availability of selection criteria, different interpretations can lead to variability in the appreciation of donor eligibility with possible viable organs missed. Our primary objective was to test the perception of feasibility of potential organ donors through the survey of a small sample of external evaluators.

Methods: Clinical scenarios summarizing 66 potential donors managed in the first year of our Organ Recovery Center were sent to four critical care physicians to evaluate the feasibility of the potential donors and the probability of organ procurement.

Results: Potential donors procuring at least one organ were identified in 55 of the 66 cases (83%). Unanimity was reached in 38 cases, encompassing 35 out of the 55 converted and 3 of the non-converted donors. The overall agreement was moderate (kappa = 0.60, 95% CI: 0.37–0.82). For the organs finally procured for transplantation, organ donation was predicted for the majority of the cases, but high discrepancy was present with the final outcome of organs not procured (particularly liver and kidney).

Conclusion: The assessment of a potential donor is a complex dynamic process. In order to increase organ availability, standardized electronically clinical data, as well a “donor board” structure of decision might inform future systems.
Post-Transplantation Early Blood Transfusion and Kidney Allograft Outcomes: A Single-Center Observational Study
Authors: Khedjat Kahina , Lenain Rémi , Hamroun Aghilès , Baes Dulciane , Top Isabelle , Labalette Myriam , Lopez Benjamin , Van Triempont Marine , Provôt François , Frimat Marie , Gibier Jean-Baptiste , Hazzan Marc , Maanaoui Mehdi
Published: May 2022

The association between blood transfusion and the occurrence of de novo HLA donor specific antibodies (DSA) after kidney transplantation remains controversial. In this single-center observational study, we examined the association between early blood transfusion, i.e. before 1-month post-transplantation, and the risk of DSA occurrence, using Luminex based-methods. In total, 1,424 patients with a minimum of 1-month follow-up were evaluated between January 2007 and December 2018. During a median time of follow-up of 4.52 years, we observed 258 recipients who had at least one blood transfusion during the first month post-transplantation. At baseline, recipients in the transfused group were significant older, more sensitized against HLA class I and class II antibodies and had a higher 1-month serum creatinine. Cox proportional hazards regression analyses did not show any significant association between blood transfusion and the risk of de novo DSA occurrence (1.35 [0.86–2.11], p = 0.19), the risk of rejection (HR = 1.33 [0.94–1.89], p = 0.11), or the risk of graft loss (HR = 1.04 [0.73–1.50], p = 0.82). These data suggest then that blood transfusion may not be limited when required in the early phase of transplantation, and may not impact long-term outcomes.
Prevalence of Blood-Borne Viruses and Predictors of Risk in Potential Organ Donors in Australia
Authors: Dutch Martin J. , Patrick Cameron J. , Boan Peter A. , Knott Jonathan C. , Opdam Helen I.
Published: May 2022

Internationally, the designation of a patient as an increased viral risk organ donor has been associated with lower utilisation rates. The actual prevalence of blood borne viruses in Australian potential organ donors, and the predictive performance of questionnaires administered to stratify this risk, remains unknown. We conducted a retrospective review of all patients who commenced workup for donation on the national database between 2014–2020. The prevalence of HIV, Active HBV and Active HCV in 3650 potential organ donors was 0.16%, 0.9%, and 2.2%, respectively. The behavioural risk profile was assessed in a subset of 3633 patients. Next-of-kin reported increased risk behaviours were associated with an increased prevalence of HCV but not of HIV or HBV (OR 13.8, p < 0.01, OR 0.3. p = 0.42, OR 1.5, p = 0.14). Furthermore, the majority of HIV and HBV infections occurred in potential donors without a disclosed history of increased risk behaviours. In this series, donors had a higher prevalence of HCV, and similar rates of HBV and HIV to the broader community. Behavioural transmission risks were poorly predictive of HIV and HBV. Rather than pre-transplantation behavioural risk screening, routine post-transplant recipient screening may provide a more powerful tool in mitigating the consequences of unexpected viral transmission.
Modelos de creación y gestión de marca. Revisión sistemática de literatura y descripción de sus fundamentos metodológicos
Authors: Montalvo-Arroyave Luisa Fernanda , Viana-Ruiz Luis Rodrigo , Arango-Lopera Carlos Andrés
Published: May 2022

Desde las últimas décadas, el tema de la marca gana interés, tanto en el ámbito académico como en el empresarial. Este interés impulsa una corriente de estudios que buscan resolver interrogantes como qué son las marcas, cómo se crean, se gestionan y se evalúan. Tales preguntas sirven como motor para originar un campo de conocimiento que arroja conceptualizaciones, teorías y modelos que circulan desde 1980. El objetivo del presente artículo es identificar y describir los modelos de creación y gestión de marcas que en la revisión sistemática de literatura figuran como los más genuinos, es decir, aquellos cuya propuesta es plenamente diferenciable de los demás. Parte de una pesquisa en las bases de datos Web of Science, SCOPUS y EBSCO, en las cuales se hallaron 366 artículos que proponían, analizaban o aplicaban modelos de gestión de marca. Luego de una depuración, se llegó a un corpus de 17 modelos, entre los que hay propuestas de corte teórico y empírico. El artículo describe tales modelos, incluye sus diagramas y los comenta a la luz de sus fundamentos conceptuales y metodológicos. Los resultados muestran un campo de conocimiento en consolidación, con notables diferencias conceptuales, diversidad de criterios y orientaciones en los modelos revisados. Se concluye que en los modelos de corte empírico prevalece un enfoque de corto plazo, una amplia reserva sobre las metodologías empleadas para su diseño y unos resultados incomparables entre sí, mientras que los modelos de corte académico, si bien se esfuerzan en la validación de sus constructos, aún están lejos de cumplir todos los criterios de cientificidad que se esperarían en productos de este tipo.
La presencia de la modelización matemática en tareas de estadística y probabilidad de libros de texto ecuatorianos
Authors: Trelles-Zambrano César , Toalongo-Guamba Ximena Patricia , Alsina-Pastells Ángel
Published: May 2022

En la actualidad la modelización matemática dentro de la investigación en ambientes educativos ha adquirido notable presencia, por ello, muchos países han decidido incorporarla tanto en sus documentos curriculares como en su práctica de enseñanza; Ecuador no es la excepción y en su última propuesta curricular la incorpora explícitamente. Los libros de texto forman parte de los recursos más utilizados por el profesorado, por tanto, el objetivo de esta investigación es determinar la presencia de la modelización matemática en el bloque de estadística y probabilidad mediante un estudio comparativo de los libros de texto entregados por el Ministerio de Educación en los años 2016-2019 frente a los libros utilizados actualmente y entregados al estudiantado desde el año 2020. La metodología utilizada responde a un enfoque cuantitativo, con un alcance descriptivo, 497 actividades fueron analizadas mediante un análisis de contenido. Los resultados muestran la poca presencia de la modelización matemática, si bien los libros actuales presentan en conjunto problemas más contextualizados, las actividades de modelización matemática han desaparecido completamente; se concluye que es necesaria una reformulación urgente de los libros de texto en lo que a estadística y probabilidad se refiere, en la que se incorpore en mayor medida actividades de modelización. Los hallazgos sirven para que el estado ecuatoriano y los autores de libros de texto tomen decisiones acerca de cómo mantener una posición coherente entre lo manifestado en los lineamientos curriculares y lo que se propone al estudiantado en su proceso de enseñanza aprendizaje a través de los libros de texto.
PSO Optimized Active Disturbance Rejection Control for Aircraft Anti-Skid Braking System
Authors: Xu Fengrui , Chen Mengqiao , Liang Xuelin , Liu Wensheng
Published: May 2022

A high-quality and secure touchdown run for an aircraft is essential for economic, operational, and strategic reasons. The shortest viable touchdown run without any skidding requires variable braking pressure to manage the friction between the road surface and braking tire at all times. Therefore, the manipulation and regulation of the anti-skid braking system (ABS) should be able to handle steady nonlinearity and undetectable disturbances and to regulate the wheel slip ratio to make sure that the braking system operates securely. This work proposes an active disturbance rejection control technique for the anti-skid braking system. The control law ensures action that is bounded and manageable, and the manipulating algorithm can ensure that the closed-loop machine works around the height factor of the secure area of the friction curve, thereby improving overall braking performance and safety. The stability of the proposed algorithm is proven primarily by means of Lyapunov-based strategies, and its effectiveness is assessed by means of simulations on a semi-physical aircraft brake simulation platform.
Influence of Fluid Viscosity and Compressibility on Nonlinearities in Generalized Aerodynamic Forces for T-Tail Flutter
Authors: Schäfer Dominik
Published: May 2022

The numerical assessment of T-tail flutter requires a nonlinear description of the structural deformations when the unsteady aerodynamic forces comprise terms from lifting surface roll motion. For linear flutter, a linear deformation description of the vertical tail plane (VTP) out-of-plane bending results in a spurious stiffening proportional to the steady lift forces, which is corrected by incorporating second-order deformation terms in the equations of motion. While the effect of these nonlinear deformation components on the stiffness of the VTP out-of-plane bending mode shape is known from the literature, their impact on the aerodynamic coupling terms involved in T-tail flutter has not been studied so far, especially regarding amplitude-dependent characteristics. This term affects numerical results targeting common flutter analysis, as well as the study of amplitude-dependent dynamic aeroelastic stability phenomena, e.g., Limit Cycle Oscillations (LCOs). As LCOs might occur below the linear flutter boundary, fundamental knowledge about the structural and aerodynamic nonlinearities occurring in the dynamical system is essential. This paper gives an insight into the aerodynamic nonlinearities for representative structural deformations usually encountered in T-tail flutter mechanisms using a CFD approach in the time domain. It further outlines the impact of geometrically nonlinear deformations on the aerodynamic nonlinearities. For this, the horizontal tail plane (HTP) is considered in isolated form to exclude aerodynamic interference effects from the studies and subjected to rigid body roll and yaw motion as an approximation to the structural mode shapes. The complexity of the aerodynamics is increased successively from subsonic inviscid flow to transonic viscous flow. At a subsonic Mach number, a distinct aerodynamic nonlinearity in stiffness and damping in the aerodynamic coupling term HTP roll on yaw is shown. Geometric nonlinearities result in an almost entire cancellation of the stiffness nonlinearity and an increase in damping nonlinearity. The viscous forces result in a stiffness offset with respect to the inviscid results, but do not alter the observed nonlinearities, as well as the impact of geometric nonlinearities. At a transonic Mach number, the aerodynamic stiffness nonlinearity is amplified further and the damping nonlinearity is reduced considerably. Here, the geometrically nonlinear motion description reduces the aerodynamic stiffness nonlinearity as well, but does not cancel it.
Geostationary Orbital Debris Collision Hazard after a Collision
Authors: Zhang Haitao , Li Zhi , Wang Weilin , Zhang Yasheng , Wang Hao
Published: May 2022

Many space objects are densely distributed in the geostationary (GEO) band, and the long-term impact of the collision of GEO spacecraft and space debris on the GEO environment has attracted more and more attention. After summarizing the advantages and disadvantages of the long-term evolution model based on the “Cube” collision probability calculation model, the “Grid” model, a long-term evolution model especially suitable for GEO band, was established. For four types of collision and disintegration events, the “Grid” model was used to study the space environment in the GEO band after collisions between GEO spacecraft and space debris. Future collisions were simulated, and the number of space objects in the next 100 years was counted. Once space debris and massive spacecraft were completely disintegrated after collision, the number of space objects and the collision probability increased sharply, and this caused a collision cascading syndrome. Even if there was no initial disintegration event, collision and disintegration events occurred in the long-term evolution of the GEO band, which led to an increase in the number of space objects. However, the collision probability was much lower, and the number of space objects grew much more slowly without the initial collision.
Design and Experiment of Horizontal Transplanter for Sweet Potato Seedlings
Authors: Yan Wei , Hu Mingjuan , Li Kun , Wang Jia , Zhang Wenyi
Published: May 2022

In view of the problems of the difficulties faced in horizontal planting; high labor intensity, poor operation quality and low economy in the existing sweet potato bare seedling transplanting technology and equipment, combined with the agronomic requirements of sweet potato planting and transplanting, a sweet potato bare seedling horizontal transplanter is designed, which can realize multiple processes such as rotary tillage and ridging, ditching and transplanting, covering soil and standing seedlings. The main parameters affecting the working performance and operation effect of the whole machine are analyzed, determining the relevant position and motion parameters, taking the forward speed of machines and tools, spacing of the ribbons and spiral speed as the influencing factors of the performance test, selecting the qualified rate of plant spacing as the evaluation index, and designing a three-factor and three-level orthogonal test. The test results show that the primary and secondary order of the significance of the qualified rate Z of plant spacing is B, A and C. The better horizontal combination of influencing factors is that the forward speed of machines and tools is 0.17 m·s−1, the spacing of the ribbons is 110 mm, and the spiral speed is 170 rpm. The field test results showed that the average Z-value of plant spacing qualification rate under the optimal factor level combination was 91.87%, which met the relevant technical standards and agronomic requirements.
Epidemiology and Genetic Diversity of Spirometra Tapeworm Isolates from Snakes in Hunan Province, China
Authors: Gong Tengfang , Su Xiaoyi , Li Fen , He Junlin , Chen Shuyu , Li Wenchao , Xie Xinrui , Liu Yisong , Zhang Xi , Liu Wei
Published: May 2022

Sparganosis, caused by the plerocercoid larvae of Spirometra tapeworms, is a public health hazard worldwide. The prevalence and genetics of sparganum from snakes remain unclear. In this study, we investigated the prevalence of sparganum infection in wild snakes in Hunan province and compared the prevalence of Spirometra tapeworms in snakes worldwide. Furthermore, the genetic diversity of collected isolates was analyzed using mitochondrial cytb and cox1 genes. The result shows that the sparganum infection rate in wild snakes (89.50%, 402/449) was higher in Hunan than in other regions. Genetic diversity analysis based on concatenated sequences revealed high genetic diversity but no distinct genetic structure among Spirometra populations. Phylogenetic analysis supported the division of European and Chinese Spirometra isolates and a single species in Chinese Spirometra isolates. The prevalence of Spirometra tapeworms in snakes is serious, and the risk of sparganosis should be further publicized.
RNA-Seq Analysis Reveals Expression Regulatory Divergence of W-Linked Genes between Two Contrasting Chicken Breeds
Authors: Gu Hongchang , Wang Liang , Lv Xueze , Yang Weifang , Chen Yu , Li Kaiyang , Zhang Jianwei , Jia Yaxiong , Ning Zhonghua , Qu Lujiang
Published: May 2022

The regulation of gene expression is a complex process involving organism function and phenotypic diversity, and is caused by cis- and trans- regulation. While prior studies identified the regulatory pattern of the autosome rewiring in hybrids, the role of gene regulation in W sex chromosomes is not clear due to their degradation and sex-limit expression. Here, we developed reciprocal crosses of two chicken breeds, White Leghorn and Cornish Game, which exhibited broad differences in gender-related traits, and assessed the expression of the genes on the W chromosome to disentangle the contribution of cis- and trans-factors to expression divergence. We found that female-specific selection does not have a significant effect on W chromosome gene-expression patterns. For different tissues, there were most parental divergence expression genes in muscle, and also more heterosis compared with two other tissues. Notably, a broader pattern of trans regulation in the W chromosome was observed, which is consistent with autosomes. Taken together, this work describes the regulatory divergence of W-linked genes between two contrasting breeds and indicates sex chromosomes have a unique regulation and expression mechanism.
Effect of a Plateau Environment on the Oxidation State of the Heart and Liver through AMPK/p38 MAPK/Nrf2-ARE Signaling Pathways in Tibetan and DLY Pigs
Authors: Hu Hong , Li Yongxiang , Yang Yuting , Xu Kexing , Yang Lijie , Qiao Shiyan , Pan Hongbin
Published: May 2022

This study evaluated the effect of a plateau environment on the heart and liver oxidation state of Tibetan pigs (TPs) and DLY pigs through analyzing AMPK, p38 MAPK, and Nrf2 signaling pathways. Twelve 120-day-old TPs and twelve 120-day-old DLY pigs were randomly divided into two groups in a plateau environment for three weeks. Exposed to a plateau environment, TPs exhibited a lower (p < 0.05) malondialdehyde level but higher (p < 0.05) glutathione, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) activities in the liver and heart than those observed in DLY pigs. TPs also showed higher (p < 0.05) mRNA levels of SOD and GSH-Px in the liver and heart compared with those of DLY pigs. The TPs showed higher (p < 0.05) mRNA and protein levels of AMPK and Nrf2 in the liver and heart compared with those of DLY pigs. Furthermore, TPs showed higher (p < 0.05) mRNA and protein levels of p38 MAPK in the heart and higher mRNA levels of p38 MAPK in the liver compared with those of DLY pigs under a plateau environment. In summary, TPs possess a stronger antioxidant capacity in the heart and liver than that of DLY pigs in a plateau environment through AMPK/p38 MAPK/Nrf2-ARE signaling pathways.
Determining the Characteristics of Farms That Raise Pigs without Antibiotics
Authors: Bernaerdt Elise , Maes Dominiek , Van Limbergen Tommy , Postma Merel , Dewulf Jeroen
Published: May 2022

Reduced and responsible antimicrobial use leads to a lower risk of developing antimicrobial resistance. Raised Without Antibiotics (RWA) is a certification label that is recognized in only a few countries, and it is often unclear what the specific criteria and characteristics of RWA farms are. The objectives of this study were to describe the criteria for a Belgian RWA program; to coach farms towards reduced antimicrobial usage (AMU); to assess if it was possible to obtain and maintain the RWA status; and to determine differences between RWA and conventional pig farms. Pig farms (n = 28) were visited three times for the following reasons: (1) data collection, (2) farm-specific coaching (2 months later), and (3) evaluation (7 months later). AMU was followed from before the start of the study up to one year after the last visit. AMU, biosecurity (Biocheck.UGentTM), and farm characteristics of (non-)RWA farms were compared. RWA was defined as no antibiotics from birth until slaughter. Pigs requiring individual treatment received a special ear tag and were excluded from the program. The status of the farms varied over time, and the distribution of RWA vs. non-RWA was 10–18, 13–15, and 12–16, before intervention, after coaching, and after one year, respectively. For the non-RWA farms, there was a reduction in AMU of 61%, 38%, and 23%, for the suckling piglets, fattening pigs, and sows, respectively, indicating that they were moving toward the RWA status. There were no significant differences in biosecurity status between RWA and non-RWA farms, but biosecurity improved in all farms throughout the study. RWA farms were smaller (median 200 sows) compared to non-RWA farms (median 350 sows). The 4-week system was used more in non-RWA farms, while the 3- and 5-week systems were used most often in RWA farms. This study showed that farmers could achieve and maintain the RWA status through farm-specific coaching related to prudent AMU and improved biosecurity.
Dietary Fish Meal Replacement with Hermetia illucens and Tenebrio molitor Larval Meals Improves the Growth Performance and Nutriphysiological Status of Ide (Leuciscus idus) Juveniles
Authors: Homska Natalia , Kowalska Joanna , Bogucka Joanna , Ziółkowska Ewa , Rawski Mateusz , Kierończyk Bartosz , Mazurkiewicz Jan
Published: May 2022

The ide (Leuciscus idus) is a native European species of rheophilic cyprinid fish whose wild population status is dependent on conservation efforts, particularly regular restocking. This study aimed to evaluate the effects of including insect meals as a component in the diet of ide juveniles on their growth performance, feed utilization, and nutriphysiological status. Four diets were formulated: three with insect meals, HI–with 20% Hermetia illucens meal, TM–with 20% Tenebrio molitor meal, and ZM–with 20% Zophobas morio meal, and the control group diet, CON–fish meal with no insect component. The effects of the various diets on the efficiency of rearing ide juveniles were assessed based on fish growth parameters, feed utilization parameters, somatic indices, and intestinal and hepatopancreatic histomorphology. The highest increase in fish weight gain and the protein efficiency ratio was observed in the HI and TM groups, while the lowest values were observed in the CON and ZM groups. Comparable results were noted for the feed conversion ratio, which was most favorable in the HI and TM groups and increased in the ZM group. The use of black soldier fly and mealworm larval meal in the diets of ide juveniles had a positive effect on rearing results and overall fish health.
Using Classical and Operant Conditioning to Train a Shifting Behavior in Juvenile False Water Cobras (Hydrodynastes gigas)
Authors: Williams Michelle L. , Torrini Lori A. , Nolan E. Joseph , Loughman Zachary J.
Published: May 2022

All animals have the capacity to learn through operant conditioning and other types of learning, and as a result, zoos and other animal care facilities have shifted towards the use of positive reinforcement training to shape the behavior of animals under their care. Training offers animals the choice to participate in their own husbandry routines and veterinary procedures, while also providing mental stimulation. By adopting these practices, the welfare of animals in human care has improved, but it has not been applied equally across taxa. Snakes are frequently overlooked in the discussion of choice and control in a captive setting, likely due to the historical misinterpretation of their intelligence and behavioral needs. In this study, a shaping plan was developed for 28 juvenile false water cobras (Hydrodynastes gigas), a rear-fanged venomous species, from four clutches. Snakes were rewarded with food when completing behaviors related to the ultimate goal of following a target into a shift container. The purpose of this study is to incorporate the trained behaviors in routine husbandry practices, while preventing unnecessary stress in the snakes and risk to the keeper.
MPO–ANCA-Positive Granulomatosis with Polyangiitis with Rapidly Progressive Glomerulonephritis and Saddle-Nose Deformity: A Case Report
Authors: Petrou Dimitra , Karagiannis Minas , Nikolopoulos Petros , Liapis George , Lionaki Sophia
Published: May 2022

Early diagnosis and initiation of appropriate immunosuppressive treatment remain the cornerstone of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis at the cost of significant toxicity. In this report, we present a case of a 69-year-old female who presented with advanced renal insufficiency and evidence of pulmonary hemorrhage and was MPO–ANCA-positive with a clinical phenotype of granulomatosis with polyangiitis. Organ involvement included rapidly progressive glomerulonephritis (GN), along with extrarenal manifestations (skin, upper and lower respiratory system involvement, and onset of saddle-nose deformity). Kidney biopsy established the diagnosis of pauci-immune crescentic, sclerotic GN. She received therapy with glucocorticoids and cyclophosphamide, mainly due to life-threatening extra-renal manifestations, such as pulmonary hemorrhage. She avoided vasculitis-related death but she developed severe therapy-related toxicity, resulting in the discontinuation of immunosuppressive therapy. Continuous re-evaluation of patients with ANCA-associated vasculitis in terms of response to immunosuppressive therapy and treatment-related toxicity is crucial for their management.
Intraosseous Regional Administration of Prophylactic Antibiotics in Total Knee Arthroplasty
Authors: Wells Zoe , Zhu Mark , Young Simon
Published: May 2022

Prosthetic joint infection (PJI) after total knee arthroplasty is a devastating complication. With the development of antibiotic resistance, a safe and effective means of delivering antibiotic prophylaxis is needed. Intraosseous regional antibiotics (IORA) achieve higher local concentrations of antibiotics with fewer systemic side effects. Previous studies have proven the safety of IORA, whereas animal models have proven it to be more effective than intravenous antibiotics for preventing infection following surgery. Recently, large-scale retrospective studies have also demonstrated lower PJI rates following TKA when IORA is used when compared to routine intravenous prophylaxis. In this article, we propose an evidence-based method for the administration of intraosseous antibiotics in TKA, cover the up-to-date data supporting its use, and explore future directions for additional research.
Urinary Biomarkers as a Proxy for Congenital Central Hypoventilation Syndrome Patient Follow-Up
Authors: Peruzzi Marta , Ramazzotti Matteo , Damiano Roberta , Vasarri Marzia , la Marca Giancarlo , Arzilli Cinzia , Piumelli Raffaele , Nassi Niccolò , Degl'Innocenti Donatella
Published: May 2022

Congenital Central Hypoventilation Syndrome (CCHS) is a rare genetic disorder of the autonomic nervous system and in particular of the respiratory control during sleep. No drug therapy is, to date, available; therefore, the survival of these patients depends on lifelong ventilatory support during sleep. Reactive oxygen species (ROS)-induced oxidative stress is a recognized risk factor involved in the pathogenesis of several chronic diseases. Therefore, monitoring systemic oxidative stress could provide important insights into CCHS outcomes. Because ROS-induced oxidative products are excreted as stable metabolites in urine, we performed an HPLC-MS/MS analysis for the quantitative determination of the three main representative oxidative biomarkers (i.e., diY, MDA, and 8-OHdG) in the urine of CCHS patients. Higher levels of urinary MDA were found in CCHS patients compared with age-matched control subjects. The noteworthy finding is the identification of urinary MDA as relevant biomarker of systemic oxidative status in CCHS patients. This study is a concise and smart communication about the impact that oxidative stress has in CCHS, and suggests the monitoring of urinary MDA levels as a useful tool for the management of these patients.
Improving the Antioxidant Activity and Flavor of Faba (Vicia faba L.) Leaves by Domestic Cooking Methods
Authors: Duan Shucheng , Kwon Soon-Jae , Gil Chan Saem , Eom Seok Hyun
Published: May 2022

Faba leaves are an unusual vegetable which contain not only a range of functional phytochemicals, but also certain undesirable flavors, which limit their consumption. In this study, several cooking methods (microwaving, roasting, steaming, and boiling), which are expected to reduce the odd flavors, were evaluated in terms of both health benefit effects and odd flavor factors, including antioxidant activities and the content of non-volatile and volatile organic compounds (VOCs). A cooking time of 5 min was selected because of the high content of l-dopa (l-3,4-dihydroxyphenylalanine) and aim of reducing the undesirable flavors of the cooked faba leaves. Microwaving and steaming significantly increased the l-dopa content by 24% and 19%, respectively. Roasting specifically increased the content of flavonols, exhibiting a 28% increase of kaempferol-3-O-arabinoside-7-O-rhamnoside, representatively, whereas boiling decreased about 50% of most phytochemicals evaluated. Microwaving and steaming treatments significantly increased the antioxidant activities. The l-dopa content and antioxidant activities of the processed faba leaves were strongly positively correlated with either an R2 = 0.863 of DPPH radical scavenging activity or an R2 = 0.856 value of ABTS radical scavenging activity, showing that l-dopa was a key antioxidant. All cooking methods potentially improved the flavor of the cooked faba leaves compared with that of the fresh leaves, because they significantly reduced the contents of VOCs such as alcohols, aldehydes, and ketones. These VOCs were the main components (>90%) in the fresh leaves. Adverse aromatic hydrocarbons were newly formed by the microwaving treatment, typically producing p-xylene, which is known to be a harmful dose-dependent compound, but they were not detected in leaves processed by the other cooking methods; therefore, although microwaving efficiently increased antioxidant activity, the chemical safety of the aromatic hydrocarbons produced need further study.
Targeting Scavenger Receptors in Inflammatory Disorders and Oxidative Stress
Authors: Bayarsaikhan Govigerel , Bayarsaikhan Delger , Lee Jaewon , Lee Bonghee
Published: May 2022

Oxidative stress and inflammation cannot be considered as diseases themselves; however, they are major risk factors for the development and progression of the pathogenesis underlying many illnesses, such as cancer, neurological disorders (including Alzheimer’s disease and Parkinson’s disease), autoimmune and metabolic disorders, etc. According to the results obtained from extensive studies, oxidative stress–induced biomolecules, such as advanced oxidation protein products, advanced glycation end products, and advanced lipoxidation end products, are critical for an accelerated level of inflammation and oxidative stress–induced cellular damage, as reflected in their strong affinity to a wide range of scavenger receptors. Based on the limitations of antioxidative and anti-inflammatory molecules in practical applications, targeting such interactions between harmful molecules and their cellular receptors/signaling with advances in gene engineering technology, such as CRISPR or TALEN, may prove to be a safe and effective alternative. In this review, we summarize the findings of recent studies focused on the deletion of scavenger receptors under oxidative stress as a development in the therapeutic approaches against the diseases linked to inflammation and the contribution of advanced glycation end products (AGEs), advanced lipid peroxidation products (ALEs), and advanced oxidation protein products (AOPPs).
A Subwavelength Transmit-Array Lens Element Combining Functions of Phase Modulation and Polarization Conversion
Authors: Xu T. , Lu Y. , He G. , Yang X.
Published: May 2022

Advanced applications require transmit-array lenses featuring low profiles, covering 360° phase modulation, and, potentially, polarization conversion. Subwavelength metamaterial elements with multilayers manipulate electromagnetic waves with predesigned phase shifts, resulting in the transmit-array lens’s low profile and light weight. Conventional designs separate the transmit-array lens and polarization converter with two functional devices and suffer from high profiles and complicated mechanical assembly. In order to resolve these issues, a subwavelength lens element combining the functions of phase modulation and polarization conversion is proposed. The metamaterial lens element consists of phase modulation, linear-circular polarization conversion modules, and a metallic via structure electrically connecting these two modules. The multifunctional lens element modulates the transmitted phase with a three-bit phase shift and operates in the frequency range from 24.5 to 27 GHz, with less than −10 dB reflection coefficient, 1 dB transmission loss, and a 2.5 dB axial ratio. A transmit-array lens antenna with the proposed element is implemented to validate the abilities of beam focus and polarization conversion. The gain of the lens antenna reaches 26.3 dBi, with a 3 dB beamwidth of 6.7° and −18 dB sidelobe level, and the axial ratio of the converted circular polarization is below 1 dB. The aperture efficiency of the antenna is 45.2%.
Machine Learning-Based Automatic Utterance Collection Model for Language Development Screening of Children
Authors: Choi Jeong-Myeong , Lee Yoon-Kyoung , Kim Jong-Dae , Park Chan-Young , Kim Yu-Seop
Published: May 2022

To assess a child’s language development, utterance data are required. The approach of recording and transcribing the conversation between the expert and the child is mostly utilized to obtain utterance data. Because data are obtained through one-on-one interactions, this approach is costly. In addition, depending on the expert, subjective dialogue situations may be incorporated. To acquire speech data, we present a machine learning-based phrase generating model. It has the benefit of being able to cope with several children, which reduces costs and allows for the collection of objectified utterance data through consistent conversation settings. Children’s utterances are initially categorized as topic maintenance or topic change, with rule-based replies based on scenarios being formed in the instance of a topic change. When it comes to topic maintenance, it encourages the child to say more by answering with imitative phrases. The strategy we suggest has the potential to reduce the cost of collecting data for evaluating children’s language development while maintaining data collection impartiality.
Extrapolation of Human Estimates of the Concreteness/ Abstractness of Words by Neural Networks of Various Architectures
Authors: Solovyev Valery , Ivanov Vladimir
Published: May 2022

In a great deal of theoretical and applied cognitive and neurophysiological research, it is essential to have more vocabularies with concreteness/abstractness ratings. Since creating such dictionaries by interviewing informants is labor-intensive, considerable effort has been made to machine-extrapolate human rankings. The purpose of the article is to study the possibility of the fast construction of high-quality machine dictionaries. In this paper, state-of-the-art deep learning neural networks are involved for the first time to solve this problem. For the English language, the BERT model has achieved a record result for the quality of a machine-generated dictionary. It is known that the use of multilingual models makes it possible to transfer ratings from one language to another. However, this approach is understudied so far and the results achieved so far are rather weak. Microsoft’s Multilingual-MiniLM-L12-H384 model also obtained the best result to date in transferring ratings from one language to another. Thus, the article demonstrates the advantages of transformer-type neural networks in this task. Their use will allow the generation of good-quality dictionaries in low-resource languages. Additionally, we study the dependence of the result on the amount of initial data and the number of languages in the multilingual case. The possibilities of transferring into a certain language from one language and from several languages together are compared. The influence of the volume of training and test data has been studied. It has been found that an increase in the amount of training data in a multilingual case does not improve the result.
Revascularization of an Immature Permanent Tooth with Periapical Periodontitis Using Concentrated Growth Factor Assisted by Erbium Laser (2940 nm) Irrigation: A Case Report
Authors: Yu Lintong , Zhou Yijie , Li Jiahui , Cheng Jing , Song Guangtai
Published: May 2022

Revascularization has been incorporated into endodontic practice and become a viable treatment alternative for immature teeth with pulp necrosis. Thorough disinfection of the root canal is a key factor for successful revascularization. An erbium: yttrium–aluminium–garnet (Er: YAG) laser has been proved advantageous for efficient root canal disinfection. This article reports one case of periapical periodontitis caused by a fractured occlusal tubercle. Revascularization assisted by Er: YAG laser irrigation was carried out with long-term follow up. During the process of treatment, we opened drainage to relieve the acute symptoms first. The necrotic pulp was removed, and then the root canal was rinsed with 0.5% sodium hypochlorite (NaOCl) solution assisted by Er: YAG laser irrigation, dried, and filled with a triple-antibiotic paste. After two weeks, the intracanal medication was removed, and 9 mL of whole vein blood was taken from the patient to prepare concentrated growth factor (CGF) after failing to induce enough blood into the canal system by over-instrumenting. The newly prepared CGF was transferred into the root canal. A 3 mm mineral trioxide aggregate (MTA) was placed directly on the thrombus of the CGF and then restored with glass-ionomer cement (GIC). The case was followed up for more than four years to record the clinical symptoms and imaging manifestations. Er: YAG irrigation is an efficient root canal disinfection protocol with the advantages of easy operation and minimal risk, which might be applicable to the revascularization of necrotic immature permanent teeth.
Genetic Diversity and DNA Fingerprinting in Broccoli Carrying Multiple Clubroot Resistance Genes Based on SSR Markers
Authors: Xie Qi , Zhao Yongyu , Liu Yumei , Han Fengqing , Liu Wei , Li Zhansheng
Published: May 2022

To identify cultivars quickly and accurately, DNA fingerprinting of 10 broccoli varieties was performed by using simple sequence repeat (SSR) marker technology. Highly informative and polymorphic SSR markers were screened using broccoli and rapeseed. Out of the 93 SSR marker pairs, 21 pairs were selected and found to have good polymorphism. Each marker pair generated 1 to 10 polymorphic bands with an average of 4.29. The average polymorphism information content (PIC) was 0.41 with a range from 0.16 to 0.95. Six selected marker pairs established the fingerprinting of the 10 accessions and their unique fingerprints. Cluster analysis of 10 accessions showed that the genetic similarity coefficient was between 0.57 and 0.91. They can be divided into 3 groups at the genetic similarity coefficient (GSC) of 0.73. The above results indicated that DNA fingerprinting could provide a scientific basis for the identification of broccoli polymerized multiple clubroot resistance genes. Research shows that SSR marker-based DNA fingerprinting further ensures plant seed purity.
Trypsin Depolarizes Pacemaker Potentials in Murine Small Intestinal Interstitial Cells of Cajal
Authors: Choi Na Ri , Kim Jeong Nam , Kim Byung Joo
Published: May 2022

Interstitial cells of Cajal (ICCs) generate pacemaker potentials in the gastrointestinal (GI) tract. In this study, the effects of trypsin on pacemaker potentials in murine small intestinal ICCs were examined. We used whole-cell patch-clamp analysis. The results of whole-cell patch-clamp analysis revealed that trypsin dose-dependently depolarized pacemaker potentials and decreased their amplitude. Treatments with the antagonists of neurokinin1 (NK1) and NK2 receptors (SR-140333 and SR-48968, respectively) slightly inhibited the trypsin-induced responses. However, treatment with the combination of SR-140333 and SR-48968 completely inhibited trypsin-induced responses. Trypsin slightly depolarized pacemaker potentials and increased their amplitude after the intracellular application of GDP-β-S. Additionally, incubation in external Ca2+-free solution inhibited trypsin-induced responses. In the presence of U-73122, staurosporine, Go6976, or xestospongin C, trypsin did not depolarize the pacemaker’s potentials. However, trypsin depolarized the pacemaker potentials in the presence of rottlerin. Finally, HC067047, a TRPV4 inhibitor, did not affect the trypsin-induced responses. These results suggest that trypsin depolarized pacemaker potentials through NK1 and NK2 receptors in the murine small intestinal ICCs, with this effect being dependent on the G protein, phospholipase C, protein kinase C, inositol triphosphate pathways, and extracellular Ca2+ but being independent of the TRPV4 pathway. Hence, trypsin-mediated GI motility regulation must be considered for prokinetic drug developments.
Experimental Investigation of Azimuth- and Sensor-Based Control Strategies for a PV Solar Tracking Application
Authors: Hariri Nasir G. , AlMutawa Mohammed A. , Osman Ibrahim Sufian , AlMadani Ibrahim K. , Almahdi Abdulilah M. , Ali Sajid
Published: May 2022

Today, the world continuously investigates the promising potential of advanced and optimized technologies for harvesting green renewable energy sources, such as a solar tracking system (STS). This study presents an actual implementation of a single-axis solar tracking system (SAST), where an azimuth control scheme is developed to precisely follow the sun’s orientation. This is demonstrated by computing in real-time the optimal azimuth angle based on the actual altitude, date, and time using an embedded microcontroller. At the same time, experimental comparisons using the azimuth-based control method compared with the proportional, integral, derivative (PID) control scheme and fixed-tilt PV systems (FTPV) are derived. Experimental results show that the developed azimuth solar tracker can autonomously follow the sun’s orientation primarily from time and location-based information and independent from the actual solar irradiance. Nevertheless, the electrical energies gained from all three tested systems revealed that using the sensor-based STS would increase the net energy production by 12.68% compared to the azimuth-based SAST, with an energy production increase of 7.7%. In addition, energy consumption by the azimuth-based STS is 65% greater than the energy consumption of a sensor-based STS due to the continuous movements of the tracker, regardless of the increase or change in power production within short periods.
An Intelligent Time-Series Model for Forecasting Bus Passengers Based on Smartcard Data
Authors: Cheng Ching-Hsue , Tsai Ming-Chi , Cheng Yi-Chen
Published: May 2022

Public transportation systems are an effective way to reduce traffic congestion, air pollution, and energy consumption. Today, smartcard technology is used to shorten the time spent boarding/exiting buses and other types of public transportation; however, this does not alleviate all traffic congestion problems. Accurate forecasting of passenger flow can prevent serious bus congestion and improve the service quality of the transportation system. To the best of the current authors’ knowledge, fewer studies have used smartcard data to forecast bus passenger flow than on other types of public transportation, and few studies have used time-series lag periods as forecast variables. Therefore, this study used smartcard data from the bus system to identify important variables that affect passenger flow. These data were combined with other influential variables to establish an integrated-weight time-series forecast model. For different time data, we applied four intelligent forecast methods and different lag periods to analyze the forecasting ability of different daily data series. To enhance the forecast ability, we used the forecast data from the top three of the 80 combined forecast models and adapted their weights to improve the forecast results. After experiments and comparisons, the results show that the proposed model can improve passenger flow forecasting based on three bus routes with three different series of time data in terms of root-mean-square error (RMSE) and mean absolute percentage error (MAPE). In addition, the lag period was found to significantly affect the forecast results, and our results show that the proposed model is more effective than other individual intelligent forecast models.
Interpreted Petri Nets Applied to Autonomous Components within Electric Power Systems
Authors: Grobelna Iwona , Szcześniak Paweł
Published: May 2022

In this article, interpreted Petri nets are applied to the area of power and energy systems. These kinds of nets, equipped with input and output signals for communication with the environment, have so far proved to be useful in the specification of control systems and cyber–physical systems (in particular, the control part), but they have not been used in power systems themselves. Here, interpreted Petri nets are applied to the specification of autonomous parts within power and energy systems. An electric energy storage (EES) system is presented as an application system for the provision of a system service for stabilizing the power of renewable energy sources (RES) or highly variable loads. The control algorithm for the EES is formally written as an interpreted Petri net, allowing it to benefit from existing analysis and verification methods. In particular, essential properties of such specifications can be checked, including, e.g., liveness, safety, reversibility, and determinism. This enables early detection of possible structural errors. The results indicate that interpreted Petri nets can be successfully used to model and analyze autonomous control components within power energy systems.