bluuebunny/crossref_metadata_2025 huggingface dataset:
Magnetoelectric and Multiferroic Properties of BaTiO3/NiFe2O4/BaTiO3 Heterostructured Thin Films Grown by Pulsed Laser Deposition Technique
Authors: Puli Venkata Sreenivas , Pradhan Dhiren Kumar , Sreenivasulu Gollapudi , Babu Simhachalam Narendra , Prasad Nandiraju Venkata , Madgula Kalpana , Chrisey Douglas B. , Katiyar Ram S.
Published: September 2021

Development of lead-free BaTiO3/NiFe2O4/BaTiO3 (BTO/NFO/BTO) trilayer structure thin films is significant for the realization of eco-friendly and implantable microelectromechanical systems (MEMS)-based devices. In the present work, we report BTO/NFO/BTO trilayer structure as a representative ferroelectric/ferromagnetic/ferroelectric (FE/FM/FE) system deposited on Pt(111)/TiO2/SiO2/Si using Pulsed Laser Deposition (PLD) technique. We report the ferroelectric, magnetic, and ME properties of BTO/NFO/BTO trilayer nanoscale heterostructure having dimensions 140/80/140 nm, at room temperature. High room temperature dielectric constant ~2145 at 100 Hz with low dielectric loss ~0.05 at 1 MHz is observed. Further, the deposited (BTO/NFO/BTO) tri-layered thin films showed magnetoelectric, multiferroic behavior with remanent polarization of 8.63 μCcm−2 at about 0.25 MV/cm and a reasonably high saturation magnetization of ~16 emu/cm3 at ~10 kOe is witnessed at room temperature. Tri-layered films have shown interesting magnetoelectric (ME) coupling coefficient (αE) ~54.5 mV/cm Oe at room temperature.
FloCan—A Revised Checklist for the Flora of the Canary Islands
Authors: Beierkuhnlein Carl , Walentowitz Anna , Welss Walter
Published: September 2021

The flora of the Canary Islands has been subject to botanical studies for more than 200 years. Several biodiversity databases are available for the archipelago. However, there are various drivers of change in real biodiversity and the knowledge about it constantly needs to be kept track of. Island floras are both: exposed to species loss and to species introductions, either through natural processes or by anthropogenic drivers. Additionally, the evolution of endemic plant species plays a substantial role. Endemic species are sensitive to population decline due to small population sizes and possible low competitiveness against incoming species. Additionally, there is continuous progress in systematics and taxonomy. Species names or their taxonomic attribution can be modified. Here, we check published plant lists for the Canary Islands and literature, and compile currently accepted taxa into an updated checklist. For this FloCan checklist, several sources were compiled, checked for completeness and quality, and their taxonomy was updated. We illustrate how far plant names are considered in regional or global databases. This work represents the current state of knowledge on Canary Island plant diversity, including introduced and recently described taxa. We provide a comprehensive and updated basis for biogeographical and macroecological studies. Particularly, the number of non-native species is being extended substantially. The adaptation to standard international nomenclature supports integration into large-scale studies.
Embryonic Development of the Avian Sternum and Its Morphological Adaptations for Optimizing Locomotion
Authors: Feneck Eleanor M. , Bickley Sorrel R. B. , Logan Malcolm P. O.
Published: September 2021

The sternum is part of the forelimb appendicular skeleton found in most terrestrial vertebrates and has become adapted across tetrapods for distinctive modes of locomotion. We review the regulatory mechanisms underlying sternum and forelimb development and discuss the possible gene expression modulation that could be responsible for the sternal adaptations and associated reduction in the forelimb programme found in flightless birds. In three phylogenetically divergent vertebrate lineages that all undertake powered flight, a ventral extension of the sternum, named the keel, has evolved independently, most strikingly in volant birds. In flightless birds, however, the sternal keel is absent, and the sternum is flattened. We review studies in a variety of species that have analysed adaptations in sterna morphology that are related to the animal’s mode of locomotion on land, in the sky and in water.
Human Activity Vibrations
Authors: Kaewunruen Sakdirat , Sresakoolchai Jessada , Huang Junhui , Harada Satoru , Wisetjindawat Wisinee
Published: September 2021

We present a unique, comprehensive dataset that provides the pattern of five activities walking, cycling, taking a train, a bus, or a taxi. The measurements are carried out by embedded sensor accelerometers in smartphones. The dataset offers dynamic responses of subjects carrying smartphones in varied styles as they perform the five activities through vibrations acquired by accelerometers. The dataset contains corresponding time stamps and vibrations in three directions longitudinal, horizontal, and vertically stored in an Excel Macro-enabled Workbook (xlsm) format that can be used to train an AI model in a smartphone which has the potential to collect people’s vibration data and decide what movement is being conducted. Moreover, with more data received, the database can be updated and used to train the model with a larger dataset. The prevalence of the smartphone opens the door to crowdsensing, which leads to the pattern of people taking public transport being understood. Furthermore, the time consumed in each activity is available in the dataset. Therefore, with a better understanding of people using public transport, services and schedules can be planned perceptively.
Experimental Data of a Hexagonal Floating Structure under Waves
Authors: Gabl Roman , Klar Robert , Davey Thomas , Ingram David M.
Published: September 2021

Floating structures have a wide range of application and shapes. This experimental investigations observes a hexagonal floating structure under wave conditions for three different draft configurations. Regular waves as well as a range of white noise tests were conducted to quantify the response amplitude operator (RAO). Further irregular waves focused on the survivability of the floating structure. The presented dataset includes wave gauge data as well as a six degree of freedom motion measurement to quantify the response only restricted by a soft mooring system. Additional analysis include the measurement of the mass properties of the individual configuration, natural frequency of the mooring system as well as the comparison between requested and measured wave heights. This allows us to use the provided dataset as a validation experiment.
Predictors of Loss of Functional Independence in Parkinson’s Disease: Results from the COPPADIS Cohort at 2-Year Follow-Up and Comparison with a Control Group
Authors: Santos García Diego , de Deus Fonticoba Teresa , Cores Bartolomé Carlos , Naya Ríos Lucía , García Roca Lucía , Martínez Miró Cristina , Canfield Hector , Jesús Silvia , Aguilar Miquel , Pastor Pau , Cosgaya Marina , García Caldentey Juan , Caballol Nuria , Legarda Inés , Hernández Vara Jorge , Cabo Iria , López Manzanares Lydia , González Aramburu Isabel , Ávila Rivera María A. , Gómez Mayordomo Víctor , Nogueira Víctor , Puente Víctor , Dotor Julio , Borrué Carmen , Solano Vila Berta , Álvarez Sauco María , Vela Lydia , Escalante Sonia , Cubo Esther , Carrillo Padilla Francisco , Martínez Castrillo Juan C. , Sánchez Alonso Pilar , Alonso Losada Maria G. , López Ariztegui Nuria , Gastón Itziar , Kulisevsky Jaime , Blázquez Estrada Marta , Seijo Manuel , Rúiz Martínez Javier , Valero Caridad , Kurtis Mónica , de Fábregues Oriol , González Ardura Jessica , Alonso Redondo Ruben , Ordás Carlos , López Díaz Luis M. , McAfee Darrian , Martinez-Martin Pablo , Mir Pablo ,   COPPADIS Study Group
Published: September 2021

Background and objective: The aim of this study was to compare the progression of independence in activities of daily living (ADL) in Parkinson’s disease (PD) patients versus a control group, as well as to identify predictors of disability progression and functional dependency (FD). Patients and Methods: PD patients and control subjects, who were recruited from 35 centers of Spain from the COPPADIS cohort between January 2016 and November 2017 (V0), were included. Patients and subjects were then evaluated again at the 2-year follow-up (V2). Disability was assessed with the Schwab & England Activities of Daily Living Scale (S&E-ADLS) at V0 and V2. FD was defined as an S&E-ADLS score less than 80%. Results: In the PD group, a significant decrease in the S&E-ADLS score from V0 to V2 (N = 507; from 88.58 ± 10.19 to 84.26 ± 13.38; p < 0.0001; Cohen’s effect size = −0.519) was observed but not in controls (N = 124; from 98.87 ± 6.52 to 99.52 ± 2.15; p = 0.238). When only patients considered functional independent at baseline were included, 55 out of 463 (11.9%) converted to functional dependent at V2. To be a female (OR = 2.908; p = 0.009), have longer disease duration (OR = 1.152; p = 0.002), have a non-tremoric motor phenotype at baseline (OR = 3.574; p = 0.004), have a higher score at baseline in FOGQ (OR = 1.244; p < 0.0001) and BDI-II (OR = 1.080; p = 0.008), have a lower score at baseline in PD-CRS (OR = 0.963; p = 0.008), and have a greater increase in the score from V0 to V2 in UPDRS-IV (OR = 1.168; p = 0.0.29), FOGQ (OR = 1.348; p < 0.0001) and VAFS-Mental (OR = 1.177; p = 0.013) (adjusted R-squared 0.52; Hosmer and Lemeshow test = 0.94) were all found to be independent predictors of FD at V2. Conclusions: In conclusion, autonomy for ADL worsens in PD patients compared to controls. Cognitive impairment, gait problems, fatigue, depressive symptoms, more advanced disease, and a non-tremor phenotype are independent predictors of FD in the short-term.
Rapid Simultaneous Testing of Multiple Antibiotics by the MALDI-TOF MS Direct-on-Target Microdroplet Growth Assay
Authors: Idelevich Evgeny A. , Nix Ilka D. , Busch Janika A. , Sparbier Katrin , Drews Oliver , Kostrzewa Markus , Becker Karsten
Published: September 2021

Accelerating antimicrobial susceptibility testing (AST) is a priority in the development of novel microbiological methods. The MALDI-TOF MS-based direct-on-target microdroplet growth assay (DOT-MGA) has recently been described as a rapid phenotypic AST method. In this proof-of-principle study, we expanded this method to simultaneously test 24 antimicrobials. An Enterobacterales panel was designed and evaluated using 24 clinical isolates. Either one or two (only for antimicrobials with the EUCAST “I” category) breakpoint concentrations were tested. Microdroplets containing bacterial suspensions with antimicrobials and growth controls were incubated directly on the spots of a disposable MALDI target inside a humidity chamber for 6, 8 or 18 h. Broth microdilution was used as the standard method. After 6 and 8 h of incubation, the testing was valid (i.e., growth control was successfully detected) for all isolates and the overall categorical agreement was 92.0% and 92.7%, respectively. Although the overall assay performance applying short incubation times is promising, the lower performance with some antimicrobials and when using the standard incubation time of 18 h indicates the need for thorough standardization of assay conditions. While using “homebrew” utensils and provisional evaluation algorithms here, technical solutions such as dedicated incubation chambers, tools for broth removal and improved software analyses are needed.
Automatic Recognition of Colon and Esophagogastric Cancer with Machine Learning and Hyperspectral Imaging
Authors: Collins Toby , Maktabi Marianne , Barberio Manuel , Bencteux Valentin , Jansen-Winkeln Boris , Chalopin Claire , Marescaux Jacques , Hostettler Alexandre , Diana Michele , Gockel Ines
Published: September 2021

There are approximately 1.8 million diagnoses of colorectal cancer, 1 million diagnoses of stomach cancer, and 0.6 million diagnoses of esophageal cancer each year globally. An automatic computer-assisted diagnostic (CAD) tool to rapidly detect colorectal and esophagogastric cancer tissue in optical images would be hugely valuable to a surgeon during an intervention. Based on a colon dataset with 12 patients and an esophagogastric dataset of 10 patients, several state-of-the-art machine learning methods have been trained to detect cancer tissue using hyperspectral imaging (HSI), including Support Vector Machines (SVM) with radial basis function kernels, Multi-Layer Perceptrons (MLP) and 3D Convolutional Neural Networks (3DCNN). A leave-one-patient-out cross-validation (LOPOCV) with and without combining these sets was performed. The ROC-AUC score of the 3DCNN was slightly higher than the MLP and SVM with a difference of 0.04 AUC. The best performance was achieved with the 3DCNN for colon cancer and esophagogastric cancer detection with a high ROC-AUC of 0.93. The 3DCNN also achieved the best DICE scores of 0.49 and 0.41 on the colon and esophagogastric datasets, respectively. These scores were significantly improved using a patient-specific decision threshold to 0.58 and 0.51, respectively. This indicates that, in practical use, an HSI-based CAD system using an interactive decision threshold is likely to be valuable. Experiments were also performed to measure the benefits of combining the colorectal and esophagogastric datasets (22 patients), and this yielded significantly better results with the MLP and SVM models.
The Contribution of QF-PCR and Pathology Studies in the Diagnosis of Diandric Triploidy/Partial Mole
Authors: Benítez Leticia , Pauta Montse , Badenas Cèlia , Madrigal Irene , Nadal Alfons , Marimon Edda , Borrell Antoni
Published: September 2021

Objective: the aim of our study was to assess the contribution of quantitative fluorescent polymerase chain reaction (QF-PCR) and pathology studies in the diagnosis of diandric triploidies/partial hydatidiform moles. Methods: this study included all fet al triploidies diagnosed by QF-PCR in chorionic villi or amniotic fluid in the 2 centers of BCNatal in which a maternal saliva sample was used to establish its parental origin. Pathology studies were performed in products of conception and concordance between a partial hydatidiform mole diagnosis and the finding of a diandric triploidy was assessed. Results: among 46 fetal triploidies, found in 13 ongoing pregnancies and in 33 miscarriages, there were 26 (56%) diandric triploidies. Concordant molecular (diandric triploidy) and pathology results (partial mole) were achieved in 14 cases (54%), while in 6 cases (23%) pathology studies were normal, and in the remaining 6 cases (23%) pathology studies could not be performed because miscarriage was managed medically. Conclusions: diandric triploidy is associated with partial hydatidiform mole and its diagnosis is crucial to prevent the development of persistent trophoblastic disease. QF-PCR analysis in chorionic villi or amniotic fluid provides a more accurate diagnosis of the parental origin of triploidy than the classical pathology studies.
Landauer’s Principle a Consequence of Bit Flows, Given Stirling’s Approximation
Authors: Devine Sean
Published: September 2021

According to Landauer’s principle, at least kBln2T Joules are needed to erase a bit that stores information in a thermodynamic system at temperature T. However, the arguments for the principle rely on a regime where the equipartition principle holds. This paper, by exploring a simple model of a thermodynamic system using algorithmic information theory, shows the energy cost of transferring a bit, or restoring the original state, is kBln2T Joules for a reversible system. The principle is a direct consequence of the statistics required to allocate energy between stored energy states and thermal states, and applies outside the validity of the equipartition principle. As the thermodynamic entropy of a system coincides with the algorithmic entropy of a typical state specifying the momentum degrees of freedom, it can quantify the thermodynamic requirements in terms of bit flows to maintain a system distant from the equilibrium set of states. The approach offers a simple conceptual understanding of entropy, while avoiding problems with the statistical mechanic’s approach to the second law of thermodynamics. Furthermore, the classical articulation of the principle can be used to derive the low temperature heat capacities, and is consistent with the quantum version of the principle.
Analyzing Difficulties in Arithmetic Word Problem Solving: An Epistemological Case Study in Primary School
Authors: Capone Roberto , Filiberti Federica , Lemmo Alice
Published: September 2021

This paper focuses on difficulties that primary school students have in facing mathematical word problems. In particular, we are interested in exploring how they develop in the transition from grade 2 to grade 5. The research basis of the hypothesis is that some difficulties detected in grade 5 are already predictable in grade 2. Starting from the data collected in grade 5 by the National Standardized Assessment, we carry out a quantitative analysis looking for word problems in which students experience difficulties. Subsequently, we conduct a backward analysis of the grade 2 test of the same cohort of students in order to identify a set of word problems linked with those selected in grade 5 test. The analysis shows the presence of many common difficulties in the two grades. We design and carry out specific educational activities concerning word problem-solving in grade 2. These activities produce positive changes in the experimental class compared to the control class. This could suggest that a previous intervention in grade 2 could allow overcoming future difficulties in word problem text comprehension in grade 5.
Enhance the Language Ability of Humanoid Robot NAO through Deep Learning to Interact with Autistic Children
Authors: She Tianhao , Ren Fuji
Published: September 2021

Autism spectrum disorder (ASD) is a life-long neurological disability, and a cure has not yet been found. ASD begins early in childhood and lasts throughout a person’s life. Through early intervention, many actions can be taken to improve the quality of life of children. Robots are one of the best choices for accompanying children with autism. However, for most robots, the dialogue system uses traditional techniques to produce responses. Robots cannot produce meaningful answers when the conversations have not been recorded in a database. The main contribution of our work is the incorporation of a conversation model into an actual robot system for supporting children with autism. We present the use a neural network model as the generative conversational agent, which aimed at generating meaningful and coherent dialogue responses given the dialogue history. The proposed model shares an embedding layer between the encoding and decoding processes through adoption. The model is different from the canonical Seq2Seq model in which the encoder output is used only to set-up the initial state of the decoder to avoid favoring short and unconditional responses with high prior probability. In order to improve the sensitivity to context, we changed the input method of the model to better adapt to the utterances of children with autism. We adopted transfer learning to make the proposed model learn the characteristics of dialogue with autistic children and to solve the problem of the insufficient corpus of dialogue. Experiments showed that the proposed method was superior to the canonical Seq2sSeq model and the GAN-based dialogue model in both automatic evaluation indicators and human evaluation, including pushing the BLEU precision to 0.23, the greedy matching score to 0.69, the embedding average score to 0.82, the vector extrema score to 0.55, the skip-thought score to 0.65, the KL divergence score to 5.73, and the EMD score to 12.21.
The Impact of Environmental Policy Stringency on Renewable Energy Production in the Visegrad Group Countries
Authors: Godawska Justyna , Wyrobek Joanna
Published: September 2021

Various environmental policy instruments supporting the development of renewable energy are used on an increasing scale as part of the policy of mitigating climate change and more. In our paper, we examine the influence of environmental policy stringency on renewable energy production in the Czech Republic, Hungary, Poland and Slovakia for the period 1993–2012 after controlling for gross domestic product per capita growth, CO2 emissions per capita and income inequality. We use the Panel Pooled Mean Group Autoregressive Distributive Lag model to analyze the long-run and the short-run relationship between restrictiveness of environmental policy and renewable energy generation. The results reveal that, in the long run, a more stringent environmental policy has a positive impact both on the increase in the absolute volume of renewable energy production, as well as on the replacement of energy from fossil sources. Our main findings indicate that renewable energy production is positively influenced not only by the stringency of instruments aimed directly at the development of this energy sector, but also by the stringency of instruments with other environmental goals and by the overall level of restrictiveness of the environmental policy.
What Drives the Eco-Friendly Tourist Destination Choice? The Indian Perspective
Authors: Nowacki Marek , Chawla Yash , Kowalczyk-Anioł Joanna
Published: September 2021

Although eco-friendly (pro-environmental) behaviour in tourism has attracted interest among practitioners and scholars, little is known about the influence of these attitudes on the choice of eco-friendly destinations, especially in the context of emerging tourist markets such as India. Thus, this article aims to verify a model of the relationships between attitudes towards the environment and eco-friendly tourism, social and personal norms regarding environmentally responsible behaviour, perceived behavioural control, behavioural intentions regarding eco-friendly destinations and the willingness to pay for such trips using the theory of planned behaviour. The study used an online survey conducted with 598 Indians. The relationships between the variables were analysed using PLS-PM. The most important results indicated that (1) there are significant relationships between the attitude towards the environment, the attitude towards an eco-friendly destination, social and personal norms and behavioural control and intentions regarding travelling to eco-destinations and (2) well-educated young Indian consumers expressed a positive attitude towards eco-friendly destinations; however, there was only a very weak relationship between this attitude and willingness to pay more for trips to them. These findings are valuable for pro-environmental planning and the growing green market/economy, as well as for the discussion on the future of pro-environmental tourism development.
Plant Regeneration via Somatic Embryogenesis in Larix principis-rupprechtii Mayr
Authors: Jiang Shuaifei , Chen Xiaoyi , Gao Ying , Cui Ying , Kong Lisheng , Zhao Jian , Zhang Jinfeng
Published: September 2021

Prince Rupprecht’s larch (Larix principis-rupprechtii Mayr) is a native conifer in North China with great economic and ecological values. Somatic embryogenesis (SE) is a powerful tool for the mass clonal propagation in plants. In this study, we described a high-efficiency SE system via indirect pathways and investigated the effect of genotype, culture conditions and phytohormones on SE. Immature zygotic embryos (IZEs) of L. principis-rupprechtii Mayr were used as explant materials. In the induction stage, embryogenic tissues (ETs) were induced on mLV medium supplemented with 2.0 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg L−1 6-benzylaminopurine (6-BA). The initiation frequencies showed significant differences (p < 0.05) among 20 genotypes of open-pollinated mother trees with the highest induction frequency reaching 30%. For tissue proliferation, proliferation in liquid medium was more efficient compared with proliferation in semi-solid medium, providing a multiplication rate of 3.12 in an 8-day subculture period. As a necessary exogenous plant growth regulator (PGR) for somatic embryo maturation in conifers, abscisic acid (ABA) was optimized at 16 mg L−1 in this system. Next, an orthogonal test on osmotic pressure factors showed 50 g L−1 sucrose, 7 g L−1 phytagel and 75 g L−1 polyethylene glycol (PEG) was the optimal combination for somatic embryo maturation in L. principis-rupprechtii Mayr. Moreover, the dispersion culture method provided a more efficient somatic embryo maturation, up to 545 per gram of fresh weight (FW). Finally, 2 g L−1 of active charcoal (AC) was found to increase the somatic embryo germination rate to 63.46%. The improved protocol of SE will serve as a foundation for establishing mass propagation and genetic transformation of L. principis-rupprechtii Mayr.
Orchard Grazing in France: Multiple Forms of Fruit Tree–Livestock Integration in Line with Farmers’ Objectives and Constraints
Authors: Paut Raphaël , Dufils Arnaud , Derbez Floriane , Dossin Anne-Laure , Penvern Servane
Published: September 2021

Although the grazing of extensive standard orchards has long been a common practice in Europe and continues to take place on a considerable portion of existing traditional orchards, it is more unusual for current specialized and intensive orchards (with bush trees) to be grazed. The way in which animals are integrated into these modern forms of orchards differs according to the animal and tree species as well as to the place relegated to livestock as well as the expected and provided ecological services of that place. However, little literature is available on these modern forms of sylvopastoralism. The objective of this paper is therefore to provide the first overview of the advantages and limitations of these systems as perceived by the actors involved. Based on several research programs, we first tracked on-farm innovations to describe a diversity of systems. We then conducted a multifactorial analysis to characterize these systems according to: (i) structural farm variables; (ii) farmer motivations to integrate livestock; (iii) technical adaptations generated by sylvopastoralism; and finally, (iv) observed services and disservices provided by livestock in orchards. A total of 34 farms and 21 variables were used to differentiate three types of systems that differed according to animal species, grazing patterns, the degree of system redesign, and compliance between initial farmer motivations and the observed services. The results showed that while the practice of livestock grazing in orchards can be agronomically effective and economically viable, its success depends on the ability of growers to integrate all of the dimensions of livestock farming into their orchard system for a win-win association. There are a large number of variables that are involved in successful orchard grazing that result in both challenges and opportunities, but success is closely linked to the grower’s ability to adapt the production system to suit the intended role of livestock and to acquire new skills. This typology paves the way for numerous combinations between orchards and livestock. The analysis of the determinants, obstacles, and benefits provided by orchard grazing provides some preliminary elements that are necessary to adapt agricultural support to a diversity of integration patterns in integrated tree and livestock systems.
Stem CO2 Efflux as an Indicator of Forests’ Productivity in Relict Juniper Woodlands (Juniperus thurifera L.) of Southern Spain
Authors: García Morote Francisco Antonio , Andrés Abellán Manuela , Rubio Eva , Pérez Anta Iván , García Saucedo Francisco , López Serrano Francisco Ramón
Published: September 2021

There are considerable uncertainties about the C cycle in semi-arid ecosystems. Hence, studies that have focused on Juniperus in Mediterranean woodlands are non-existent. This study provides a survey of the effect of the juniper woodland type (young and mature woodlands; joint effect of maturity and forest productivity) on stem respiration. We checked the seasonal variation of stem respiration, evaluating the effects of stem temperature on stem CO2 efflux. For this, we measured the stem CO2 efflux (µmol CO2 m−2 s−1) over the four seasons on 16 junipers using LI-6400 equipment. The results showed that in the more productive site (young woodland), the stem CO2 efflux was higher. This variable followed a clear seasonal trend, being higher during the spring and progressively decreasing in cold periods. In both juniper woodlands, and especially in the older forests, the Q10 coefficients were low (<2), typical of cold forests and slow-growing species. The exponential model also confirmed that the Q10 was significantly higher in young juniper trees. Thus, stem CO2 efflux was an indicator of the growth in this juniper woodland that is well adapted to a semi-arid climate.
Variability in Carbon Stocks across a Chronosequence of Masson Pine Plantations and the Trade-Off between Plant and Soil Systems
Authors: He Jie , Dai Quanhou , Xu Fengwei , Peng Xudong , Yan Youjin
Published: September 2021

Plantations sequester atmospheric carbon dioxide and positively respond to climate change, but the carbon (C) sequestration capacity and the trade-off between plant and soil systems in plantations may vary significantly across a chronosequence. Masson pine (Pinus massoniana Lamb.) plantations were selected to investigate the variability of C stocks in 7-, 14-, and 30-year-old stands. The total ecosystem C stock increased with stand age from 14.82 to 19.21 Mg C. Carbon stocks increased with stand age in the plant system but decreased in the soil system, with the ratio of plant-to-soil C stocks increasing from 0.06 in the 7-year-old plantation to 0.70 in the 30-year-old plantation. Carbon stocks in the first 20 cm of the soil accounted for 44.60%, 43.01%, and 30.18% of the total ecosystem carbon stock in 7-, 14-, and 30-year-old plantations, respectively. The variation trends for the proportions of C stock in soil decreased with soil depth as a result of tree and root growth regardless of stand age. Most C was stored in the stems, which contributed 1.36%, 6.85%, and 29.57% of total ecosystem C stock across the chronosequence. Results of structural equation model indicated that the effect of plant system C stock on ecosystem C stock was far larger than soil system C stock, and saturated hydraulic conductivity (ks) and fractal dimension (D) could be the primary parameters affecting ecosystem C stocks according to redundancy analysis (Variance explained by the variables selected). In summary, the plant system increased biomass C stocks by regulating soil properties to meet their growth requirements, the growth of plants in turn changed the soil organic carbon (SOC) stock, then both regulated ecosystem carbon sequestration in Masson pine plantations.
A Retrospective Analysis of the COVID-19 Infodemic in Saudi Arabia
Authors: Alasmari Ashwag , Addawood Aseel , Nouh Mariam , Rayes Wajanat , Al-Wabil Areej
Published: September 2021

COVID-19 has had broad disruptive effects on economies, healthcare systems, governments, societies, and individuals. Uncertainty concerning the scale of this crisis has given rise to countless rumors, hoaxes, and misinformation. Much of this type of conversation and misinformation about the pandemic now occurs online and in particular on social media platforms like Twitter. This study analysis incorporated a data-driven approach to map the contours of misinformation and contextualize the COVID-19 pandemic with regards to socio-religious-political information. This work consists of a combined system bridging quantitative and qualitative methodologies to assess how information-exchanging behaviors can be used to minimize the effects of emergent misinformation. The study revealed that the social media platforms detected the most significant source of rumors in transmitting information rapidly in the community. It showed that WhatsApp users made up about 46% of the source of rumors in online platforms, while, through Twitter, it demonstrated a declining trend of rumors by 41%. Moreover, the results indicate the second-most common type of misinformation was provided by pharmaceutical companies; however, a prevalent type of misinformation spreading in the world during this pandemic has to do with the biological war. In this combined retrospective analysis of the study, social media with varying approaches in public discourse contributes to efficient public health responses.
Small Alcohols as Surfactants and Hydrate Promotors
Authors: Kvamme Bjørn
Published: September 2021

Many methods to produce hydrate reservoirs have been proposed in the last three decades. Thermal stimulation and injection of thermodynamic hydrate inhibitors are just two examples of methods which have seen reduced attention due to their high cost. However, different methods for producing hydrates are not evaluated thermodynamically prior to planning expensive experiments or pilot tests. This can be due to lack of a thermodynamic toolbox for the purpose. Another challenge is the lack of focus on the limitations of the hydrate phase transition itself. The interface between hydrate and liquid water is a kinetic bottle neck. Reducing pressure does not address this problem. An injection of CO2 will lead to the formation of a new CO2 hydrate. This hydrate formation is an efficient heat source for dissociating hydrate since heating breaks the hydrogen bonds, directly addressing the problem of nano scale kinetic limitation. Adding limited amounts of N2 increases the permeability of the injection gas. The addition of surfactant increases gas/water interface dynamics and promotes heterogeneous hydrate formation. In this work we demonstrate a residual thermodynamic scheme that allows thermodynamic analysis of different routes for hydrate formation and dissociation. We demonstrate that 20 moles per N2 added to the CO2 is thermodynamically feasible for generating a new hydrate into the pores. When N2 is added, the available hydrate formation enthalpy is reduced as compared to pure CO2, but is still considered sufficient. Up to 3 mole percent ethanol in the free pore water is also thermodynamically feasible. The addition of alcohol will not greatly disturb the ability to form new hydrate from the injection gas. Homogeneous hydrate formation from dissolved CH4 and/or CO2 is limited in amount and not important. However, the hydrate stability limits related to concentration of hydrate former in surrounding water are important. Mineral surfaces can act as hydrate promotors through direct adsorption, or adsorption in water that is structured by mineral surface charges. These aspects will be quantified in a follow-up paper, along with kinetic modelling based on thermodynamic modelling in this work.
Antioxidant and Angiotensin-Converting Enzyme (ACE) Inhibitory Activities of Yogurt Supplemented with Lactiplantibacillus plantarum NK181 and Lactobacillus delbrueckii KU200171 and Sensory Evaluation
Authors: Kim Eun-Deok , Lee Hyun-Sook , Kim Kee-Tae , Paik Hyun-Dong
Published: September 2021

This study was carried out to develop a functional yogurt with inhibitory effects on angiotensin-converting enzyme (ACE) and antioxidant activity using various probiotic strains. Yogurts were prepared using a commercial LAB freeze-dried product and probiotics.Yogurt with only commercial LAB product as control group (C) and probiotics supplemented with Lacticaseibacillus rhamnosus GG KCTC 12202 BP, as a reference group (T1), Lactiplantibacillus plantarum KU15003 (T2), Lactiplantibacillus plantarum KU15031 (T3), Lactiplantibacillus plantarum NK181 (T4), and Lactobacillus delbrueckii KU200171 (T5). The T5 sample showed high antioxidant activities (86.5 ± 0.3% and 39.3 ± 1.0% in DPPH and ABTS assays, respectively). The T4 sample had the highest ACE inhibitory activity (51.3 ± 10.3%). In the case of sensory evaluation, the T4 and T5 samples did not show a significant difference (p > 0.05) compared to the reference group. These results suggest that L. plantarum NK181 and L. delbrueckii KU200171 can be used in the food industry especially dairy to improve health benefits for hypertensive patients.
Medicines in the Kitchen: Gender Roles Shape Ethnobotanical Knowledge in Marrakshi Households
Authors: Teixidor-Toneu Irene , Elgadi Sara , Zine Hamza , Manzanilla Vincent , Ouhammou Ahmed , D’Ambrosio Ugo
Published: September 2021

Differences in gendered knowledge about plants are contingent on specific cultural domains. Yet the boundaries between these domains, for example food and medicine, are sometimes blurred, and it is unclear if and how gender plays a role in creating a continuum between them. Here, we present an in-depth evaluation of the links between gender, medicinal plant knowledge, and culinary culture in Marrakech, Morocco. We interviewed 30 women and 27 men with different socio-demographic characteristics and evaluated how gender and cooking frequency shape their food and medicinal plant knowledge. We documented 171 ethno-taxa used in Marrakshi households as food, medicine, or both, corresponding to 148 botanical taxa and three mixtures. While no clear differences appear in food plant knowledge by gender, women have a three-fold greater knowledge of medicinal plants, as well as plants with both uses as food and medicine. Women’s medicinal and food plant knowledge increases with their reported frequency of cooking, whereas the opposite trend is observed among men. Men who cook more are often single, have university-level degrees, and may be isolated from the channels of knowledge transmission. This demonstrates that the profound relations between the culinary and health domains are mediated through gender.
Prebiotic Potential of Cereal Components
Authors: Abdi Reihane , Joye Iris J.
Published: September 2021

One type of functional food that has been receiving much attention is food rich in prebiotics. The old but still valid definition of prebiotics defines them as non-digestible food components that selectively stimulate the growth and/or activity of the beneficial bacteria in the colon and, as a result, improve the host health. Cereals, as one of the main components in the human diet, contain substantial levels of dietary fiber with probable prebiotic potential. In addition, dietary fiber, particularly soluble dietary fiber, has recently emerged as a promising natural highly functional food ingredient in food production. This review focuses on the prebiotic potential of cereal dietary fiber types and covers the achievements and developments regarding its isolation. First, the probiotic and prebiotic concepts will be discussed. Next, different components of dietary fiber and their effect on the host bacteria through in vitro and/or in vivo studies will be reviewed. In a last part, this paper also discusses means of boosting the prebiotic properties of cereal components and innovative strategies for the extraction of cereal dietary fiber. The review focuses on wheat as a leading cereal crop that is widely and intensely used throughout the world in food production.
Tandem Mass Tag-Based Quantitative Proteomic Analysis of ISG15 Knockout PK15 Cells in Pseudorabies Virus Infection
Authors: He Wenfeng , Li Chen , Dong Liangliang , Yang Guoqing , Liu Huimin
Published: September 2021

Pseudorabies virus (PRV) is recognized as one of the most important pathogens of swine and poses a serious threat to the swine industry worldwide. Available commercial vaccines fail to protect against the emergence of new PRV strains. Therefore, the new protein targets against PRV highlight the urgent need for uncovering the molecular determinants of host cellular proteins following PRV infection. Interferon-stimulated gene 15 (ISG15) demonstrates an outstanding antiviral response. However, the molecular mechanism of ISG15 that affects PRV replication is incompletely known. Here, we performed a tandem mass tag (TMT)-based approach to quantitatively identify protein expression changes in PRV-infected ISG15 knockout PK15 (ISG15−/−-PK15) cells. In total, 4958 proteins were identified by using TMT coupled with LC-MS/MS in this study. In the PRV- and mock-infected groups, 241 differentially expressed proteins (DEPs) were identified, 162 upregulated and 79 downregulated proteins at 24 h post-infection (hpi), among which AFP, Vtn, Hsp40, Herc5, and Mccc1 may play important roles in PRV propagation. To ensure the validity and reliability of the proteomics data, the randomly selected DEPs were verified by RT-qPCR and Western blot analysis, and the results were consistent with the TMT results. Bioinformatics analyses further demonstrated that the DEPs are mainly involved in various biological processes and signaling pathways, such as signal transduction, the digestive system, and the PI3K-AKT pathway. These findings may provide new insight into molecular mechanisms for PRV infection, which is helpful for identifying potential protein targets for antiviral agents.
Genomic Tools for the Identification of Loci Associated with Facial Eczema in New Zealand Sheep
Authors: McRae Kathryn M. , Rowe Suzanne J. , Johnson Patricia L. , Baird Hayley J. , Cullen Neil G. , Bixley Matthew J. , Plowman Jeffrey E. , Deb-Choudhury Santanu , Brauning Rudiger , Amyes Neville C. , Dodds Ken G. , Newman Sheryl-Anne N. , McEwan John C. , Clarke Shannon M.
Published: September 2021

Facial eczema (FE) is a significant metabolic disease that affects New Zealand ruminants. Ingestion of the mycotoxin sporidesmin leads to liver and bile duct damage, which can result in photosensitisation, reduced productivity and death. Strategies used to manage the incidence and severity of the disease include breeding. In sheep, there is considerable genetic variation in the response to FE. A commercial testing program is available for ram breeders who aim to increase tolerance, determined by the concentration of the serum enzyme, gamma-glutamyltransferase 21 days after a measured sporidesmin challenge (GGT21). Genome-wide association studies were carried out to determine regions of the genome associated with GGT21. Two regions on chromosomes 15 and 24 are reported, which explain 5% and 1% of the phenotypic variance in the response to FE, respectively. The region on chromosome 15 contains the β-globin locus. Of the significant SNPs in the region, one is a missense variant within the haemoglobin subunit β (HBB) gene. Mass spectrometry of haemoglobin from animals with differing genotypes at this locus indicated that genotypes are associated with different forms of adult β-globin. Haemoglobin haplotypes have previously been associated with variation in several health-related traits in sheep and warrant further investigation regarding their role in tolerance to FE in sheep. We show a strategic approach to the identification of regions of importance for commercial breeding programs with a combination of discovery, statistical and biological validation. This study highlights the power of using increased density genotyping for the identification of influential genomic regions, combined with subsequent inclusion on lower density genotyping platforms.
“A Sustained, Productive, Constructive Relationship with Someone Who Can Help”—A Qualitative Exploration of the Experiences of Help Seekers and Support Persons Using the Emergency Department during a Suicide Crisis
Authors: Rheinberger Demee , Macdonald Diane , McGillivray Lauren , Maple Myfanwy , Torok Michelle , Nicolopoulos Alexandra , Shand Fiona
Published: September 2021

For Australians experiencing a suicide crisis, the emergency department (ED) is the recommended point of contact for intervention and to ensure personal safety. However, negative ED experiences can deter individuals from returning, thus impacting future suicide risk. In order to improve the ED environment for individuals in suicidal crisis, an in-depth understanding of this experience is needed. In-depth semi-structured interviews with 17 help seekers and 16 support persons were conducted. A grounded theory approach uncovered a core organising concept—all participants wanted a “a sustained, productive, constructive relationship with someone who can help” during the ED visit—which guided analysis. Thematic analysis resulted in two themes and four subthemes exploring the systemic and interpersonal aspects of the ED visit and the roadblocks and pathways to development of the relationship. Interpersonal factors included aspects of staff interaction and presence of a support person. Systemic factors related to aspects controlled by the physical space and internal policies and procedures and included aspects such as the chaotic environment, long waiting times, and access to staff. Overwhelmingly, there were more roadblocks than pathways reported by participants. Improving the ED environment, increasing staff training and encouraging the presence of support persons may help mitigate some of these roadblocks.
There Are Predictors of Eating Disorders among Internet Use Characteristics—A Cross-Sectional Study on the Relationship between Problematic Internet Use and Eating Disorders
Authors: Kożybska Marta , Radlińska Iwona , Czerw Aleksandra , Dykowska Grażyna , Karakiewicz Beata
Published: September 2021

The aims of this cross-sectional study were: (i) to establish the prevalence of problematic Internet use (PIU) and eating disorders (EDs) among Polish students; (ii) to investigate potential correlations between the two phenomena; and (iii) to identify predictors of eating disorders among socio-demographic and Internet use characteristics in this population. To this end, a total of 1008 Polish students aged 18–40, completed the Problematic Internet Use Test (TPIU22), the Eating Attitudes Test (EAT-26) and a self-designed Socio-demographic and Internet Use Survey. Men received more PIU scores (p < 0.001), while women received more EAT-26 scores (p < 0.05) with a significant correlation observed between those variables (rho = 0.212; p < 0.001). The strongest predictors of EDs were as follows: preoccupation with the Internet, neglect of sleep in favor of Internet use, alleviation of negative feelings while online, higher mean number of hours spent online on weekends for academic and work-related purposes, extracurricular activity, lower height and higher BMI. An association has been demonstrated between problematic internet use and eating disorders. Somewhat surprisingly, our results suggest that people at risk of EDs use the Internet primarily to fulfill their routine duties. Nevertheless, further research is needed to establish the causality of EDs and PIU.
Long-Term Survival and Medical Costs of Patients with Prolonged Mechanical Ventilation and Tracheostomy: A Nationwide Cohort Study
Authors: Lai Hui-Hsuan , Tseng Pei-Ying , Wang Chen-Yu , Wang Jong-Yi
Published: September 2021

Few large-scale studies have focused on tracheostomy in patients with prolonged mechanical ventilation. This retrospective population-based study extracted data from the longitudinal National Health Insurance Research Database in Taiwan to compare long-term mortality between patients on prolonged mechanical ventilation with and without tracheostomy and their related medical expenditures. Data on newly developed respiratory failure in patients on ventilator support were extracted from 1 January 2002 to 31 December 2008. Of 10,705 patients included, 1372 underwent tracheostomy (n = 563) or translaryngeal intubation (n = 779). Overall survival of the patients with tracheostomy was followed for 5 years. Average survival was 4.98 years for the patients with tracheostomy and 5.48 years for the patients with translaryngeal intubation (not significant). Sex, age, premium-based monthly salary difference, occupation, urbanization level, chronic obstructive pulmonary disease, chronic heart failure, chronic renal disease, and cerebrovascular diseases were significantly associated with mortality for endotracheal intubation. Male sex, chronic heart failure, chronic renal disease, age ≥45 years, and low income were associated with significantly higher mortality. Although total medical expenditures were higher for the patients with tracheostomy, annual medical expenditures were not significantly different. There were no differences in long-term mortality between the two groups.