bluuebunny/crossref_metadata_2025 huggingface dataset:
Predictability of the Spatiotemporal Pattern of Wild Boar (Sus scrofa) Rooting Influenced by Acorn Availability
Authors: Sütő Dávid , Siffer Sándor , Farkas János , Katona Krisztián
Published: November 2023

The natural regeneration of the temperate oak forests is often insufficient. Acorns of the oak serve as the basis of the recruitment and key food resources in these ecosystems, thus the crop size, the germination success and seed predators have crucial roles in the process. Wild boar (Sus scrofa) is often considered as one of the main mitigating agents in oak regeneration. Therefore, in our study we analyzed and compared the spatial patterns of the acorn density and the patches rooted by wild boar within and among the different examined time intervals in a 28 ha Turkey-sessile oak (Quercus cerris, Q. petraea) forest stand. Data were collected between 2016 October and 2019 December. In the acorn density patterns, intra-annual similarities were recognized mainly, regardless of the crop size. Meanwhile, rooting patterns showed inter- and intra-annual similarities in mast years and intra-annual overlaps in non-mast years, indicating that masting is a fundamental driver of wild boar foraging behavior. However, a direct local connection between the rooting intensity and the acorn density could not be shown, as wild boars never fully depleted the acorns, even in intensively used patches. This study can help in predicting the intensively rooted forest patches, providing opportunities to manage wildlife conflicts.
The Composite Physiological Response of Hydraulic and Photosynthetic Traits and Nonstructural Carbon in Masson Pine Seedlings to Drought Associated with High Temperature
Authors: Fang Dongming , Yao Heting , Huang Yuelai , Li Weijiao , Mei Tingting , Jin Songheng
Published: November 2023

Masson pine (Pinus massoniana Lamb.) is a dominant coniferous species in southern China, known for its rapid growth, abundant yield, and extensive utilization. Despite the robust adaptability of Masson pine and the rich annual precipitation in its distribution areas, this species still faces the mortality risk caused by the recurrent high temperatures in summer and low precipitation in subtropical regions. The mortality risk of Masson pine may increase in the future when facing a more frequent or intensive drought threat due to climate change. In this study, we conducted a manipulated drought experiment accompanying high temperature (~32.3 ± 0.7 °C in daytime and 28 °C in nighttime) to simulate a flash drought, aiming to explore the composite physiological response (hydraulic, gas exchange, and nonstructural carbon (NSC) characteristics) of Masson pine seedlings to extreme drought characterized by a high intensity and long duration. We found that, as the drought developed, the leaf water potential and gas exchange traits (net photosynthesis rate, stomatal conductance, and transpiration) significantly decreased while the percentage loss of hydraulic conductivity (PLC) significantly increased. In contrast, NSC remained a more constant trend before it was significantly reduced on day 30 after the beginning of the drought. Except for NSC, all the other traits had significant correlations between them. Additionally, hydraulic dysfunction indicated by the increasing PLC preceded the NSC depletion, which may indicate a more significant role for hydraulic failure than carbon starvation in drought-induced mortality. Conclusively, hydraulic and gas exchange traits showed a coupling response to drought, but NSC displayed an independent dynamic. The findings may improve our understanding of drought-coping strategies of Masson pine and provide some theoretical basis for Masson pine forest management.
Improving Recycled Paper Materials through the Incorporation of Hemp, Wood Virgin Cellulose Fibers, and Nanofibers
Authors: Filipova Inese , Andze Laura , Skute Marite , Zoldners Juris , Irbe Ilze , Dabolina Inga
Published: November 2023

A significant increase in the consumption of recycled fiber products has been observed worldwide, and the industry is forced to solve the challenges of recycled fiber quality and add strength agents and/or virgin fibers to reach sufficient properties. In order to investigate whether the mechanical and air permeability properties of waste fiber (WF) material can be significantly improved by adding wood kraft fibers (KF), hemp soda fibers (HF), and ammonium persulfate oxidated cellulose nanofibrils (CNF), different fiber blends were prepared and tested. Results revealed the excellence of hemp fibers over wood fibers regarding the improvement of WF products. The results of WF after the addition of 10% mixed fibers (KF + HF) were higher than the results of a 50% KF addition. The impact of CNF depended on fiber composition and properties. A formula for modeling the CNF impact on different fiber compositions was proposed. Obtained fiber material samples showed suitability for home composting, thus contributing to the goals of the European Green Deal regarding reducing landfill waste and the development of cleaner products.
Fungi and Actinobacteria: Alternative Probiotics for Sustainable Aquaculture
Authors: Ghosh Koushik , Harikrishnan Ramasamy , Mukhopadhyay Abhisek , Ringø Einar
Published: November 2023

Aquatic animals are continually being exposed to stressors under farming conditions, increasing risks to gut health that result in dysbiosis. Since restrictions are imposed on the frequent use of antibiotics in aquaculture, there is emerging demand for economically viable, environmentally safe, and sustainable alternatives for the intensive production of aquaculture species. The application of beneficial microorganisms as probiotics has been suggested and widely practiced in recent times. Bacteria and fungi are ubiquitous microorganisms that can grow in various environments where organic substrates are available. Being rich in nutrients, a fish’s aquatic environment and gastrointestinal tract confer a favorable culture milieu for the microorganisms. However, the colonization and probiotic potential of fungi and mycelial bacteria resembling fungi (actinobacteria), either in the culture environment or within the gastrointestinal tracts of fish, have received less emphasis. Apart from bacilli and lactic acid bacteria, as the most conventionally used probiotics in aquaculture, numerous studies have focused on other promising alternatives. Diverse species of yeasts and molds belonging to the kingdom ‘Fungi’ have been characterized for their prospective roles in nutrition, immunomodulation, and disease prevention in fish. Bioactive compounds such as manno-oligosaccharides and β-glucans are recognized as fungal postbiotics that improve innate immunity and disease resistance in fish. Actinobacteria are known to possess different hydrolytic enzymes and novel secondary metabolites representing their probiotic attributes. The application of these groups in water quality amelioration has also been explored. Thus, this paper presents an overview of the present status of knowledge pertaining to the effects of yeasts (Candida, Cryptococcus, Debaryomyces, Geotrichum, Leucosporidium, Pichia, Rhodosporidium, Rhodotorula, Saccharomyces, Sporidiobolus, Sporobolomyces, Trichosporon and Yarrowialipolytica), molds (Aspergillus spp.) and actinobacteria (Streptomyces) as probiotics in finfish aquaculture, as well as their occurrence within the gastrointestinal tracts of finfish. Furthermore, probiotic mechanisms, selection criteria, and future perspectives on using fungi and actinobacteria as promising probiotics are discussed.
Molecular Characterization and Antibacterial Potential of Goose-Type Lysozyme from Japanese Pufferfish (Takifugu rubripes)
Authors: Cao Xinyu , Yang Zhen , Gao Minghong , Yang Xu , Wang Shuhui , Zhao Ruihu , Chen Lei , Jiang Chen , Wang He
Published: November 2023

Lysozyme plays a crucial role in the innate immune response against bacterial phagocytosis by hydrolyzing the peptidoglycan layer of the bacterial cell wall. In this study, we characterized a goose-type lysozyme gene (TrLysG) in Japanese pufferfish. It is made up of an ORF of 573 bp that encodes a polypeptide of 190 amino acids. TrLysG includes a characteristic bacterial soluble lytic transglycosylase (SLT) domain, which contains three catalytic residues (Glu71, Asp84 and Asp95) and a highly conserved GLMQ motif (Gly90, Leu91, Met92 and Gln93). Phylogenetic analyses revealed that TrLysG is clustered together with its counterparts from other teleost fishes. Furthermore, mRNA expression analyses showed that TrLysG was highly expressed in healthy mucosal tissues (intestines and gills), and considerably up-regulated in response to Vibrio harveyi infection in the intestines, gills, and liver. At pH 6 and 55 °C, the pure recombinant TrLysG (rTrLysG) exhibits optimum activity. It also displayed antimicrobial activity against three Gram-positive bacteria (Streptococcus parauberis, Staphylococcus pasteuri and Staphylococcus epidermidis) as well as five Gram-negative bacteria (Shewanella, Aeromonas hydrophila, Escherichia coli, Vibrio parahaemolyticus and V. harveyi). Our results highlighted the significant role of TrLysG in immune defense against invading pathogens, thereby contributing to the prevention and alleviation of disease spread in aquaculture.
Portable Air Purifiers’ Predicted Efficacy in Mitigating Airborne Pathogen Transmission in an Office Room Featuring Mixing Ventilation
Authors: Li Xiangdong , Patel Milan J. , Cole Ivan S.
Published: November 2023

Portable air purifiers have been extensively used to improve indoor air quality and mitigate the transmission of airborne diseases. However, the efficacy of mitigation is strongly affected by the interactions between jet flows of processed air from the air purifiers and the background airflows driven by the ventilation system. Critical factors in this context include the position and capacity of air purifiers and the ventilation rate of the heating ventilation and air-conditioning (HVAC) system. These factors are investigated in this study via computational fluid dynamics (CFD) simulations and the infection probability for different scenarios is quantified using the latest airborne infection predictive model incorporating recent pathological and clinical data for SARS-CoV-2. The results show that the use of air purifiers can significantly reduce the concentration of particulate matter, thus contributing to a generally lower risk of airborne transmission. However, the position of air purifiers affects their overall efficacy remarkably. Comparatively, a central HVAC system is more efficient at removing airborne particles under an equivalent ventilation rate assuming it uses a mixing ventilation scheme.
1H NMR Metabolomics on Pigs’ Liver Exposed to Antibiotics Administration: An Explorative Study
Authors: Fabrile Maria Pia , Ghidini Sergio , Caligiani Augusta , Scali Federico , Varrà Maria Olga , Lolli Veronica , Alborali Giovanni Loris , Ianieri Adriana , Zanardi Emanuela
Published: November 2023

An untargeted Nuclear Magnetic Resonance (NMR) spectroscopy-based metabolomics approach was applied as a first attempt to explore the metabolome of pigs treated with antibiotics. The final goal was to investigate the possibility of discriminating between antibiotic-treated (TX group) and untreated pigs (CTRL group), with the further perspective of identifying the authentication tools for antibiotic-free pork supply chains. In particular, 41 samples of pig liver were subjected to a biphasic extraction to recover both the polar and the non-polar metabolites, and the 1H NMR spectroscopy analysis was performed on the two separate extracts. Unsupervised (principal component analysis) and supervised (orthogonal partial least squares discriminant analysis) multivariate statistical analysis of 1H NMR spectra data in the range 0–9 ppm provided metabolomic fingerprinting useful for the discrimination of pig livers based on the antibiotic treatment to which they were exposed. Moreover, within the signature patterns, significant discriminating metabolites were identified among carbohydrates, choline and derivatives, amino acids and some lipid-class molecules. The encouraging findings of this exploratory study showed the feasibility of the untargeted metabolomic approach as a novel strategy in the authentication framework of pork supply chains and open a new horizon for a more in-depth investigation.
Echinacea purpurea (L.) Moench Polysaccharide Alleviates DSS-Induced Colitis in Rats by Restoring Th17/Treg Balance and Regulating Intestinal Flora
Authors: Li Yaoxing , Lin Yongshi , Zheng Xirui , Zheng Xiaoman , Yan Mingen , Wang Huiting , Liu Cui
Published: November 2023

Echinacea purpurea is popularly used as a food supplement or nutritional supplement for its immune regulatory function against various threats. As one of its promising components, Echinacea purpurea (L.) Moench polysaccharide (EPP) has a wide range of biological activities. To evaluate the effect of EPP as a dietary supplement on ulcerative colitis (UC), this study used sodium dextran sulfate (DSS) to induce a UC model, extracted EPP using the ethanol subsiding method, and then supplemented with EPP by gavage for 7 days. Then, we evaluated the efficacy of EPP on DSS rats in terms of immunity, anti-inflammation, and intestinal flora. The result showed that EPP could alleviate colonic shortening and intestinal injury in rats with DSS-induced colitis, decrease the disease activity index (DAI) score, downregulate serum levels of inflammatory cytokines, and contribute to the restoration of the balance between the T helper cells 17 (Th17) and the regulatory T cells (Treg) in the spleen and mesenteric lymph nodes (MLNs). Meanwhile, EPP could downregulate the expression of Toll-like receptors 4 (TLR4), myeloid differentiation factor 88 (MyD88), and nuclear factor kappa-B (NF-κB) in colon tissue. In addition, the results of 16SrRNA sequencing showed that EPP also had a regulatory effect on intestinal flora of UC rats. These results indicate that EPP might achieve a beneficial effect on UC rats as a dietary supplement through restoring Th17/Treg balance, inhibiting the TLR4 signaling pathway and regulating intestinal flora, suggesting its possible application as a potential functional food ingredient alleviating UC.
Non-Sensory Perception and Sensory Appeal of Zamnè, PseudoZamnè, Traditionally Cooked Senegalia erythrocalyx Seeds, and Tempeh According to Burkinabe Consumers
Authors: Drabo Moustapha Soungalo , Traoré Korotimi , Parkouda Charles , Hama-Ba Fatoumata , Savadogo Aly , Raes Katleen
Published: November 2023

The lack of adequate knowledge of the culinary and sensory properties of most indigenous and wild foods hampers their promotion in human diets and the market. In the present study, 80 Burkinabe volunteers evaluated the sensory appeal and attributes of three selected Senegalia seed species (Zamnè, pseudoZamnè or Kumatiya, and S. erythrocalyx) and their food formulae (traditionally cooked, harvested as green and fresh legumes, and fermented as tempeh) using the nine-point hedonic scale and check-all-that-apply questionnaire. They found that the traditionally cooked Zamnè, pseudoZamnè or Kumatiya, and derived tempeh had good sensory appeal (scoring between 5 and 7) and subtle alkaline and nutty tastes. However, an appreciable number (32%) of the participants were unaccustomed to tempeh and gave very low scores (2.5–3.4) for all the tempeh products. In contrast, the traditionally cooked seeds of Senegalia erythrocalyx and the green and fresh Zamnè evoked bitter and sour off-tastes, respectively, and were not much appreciated (scoring 4). The present study provides unprecedented insight into consumers’ non-sensory perceptions and the culinary and sensory properties of Senegalia seed foods, which will be essential for their valorization, branding, and marketing.
Atomization of Cocoa Honey Using Whey Protein Isolate to Produce a Dry Formulation with Improved Shelf Life for Industrial Application
Authors: Guirlanda Christiano Pedro , Alvim Izabela Dutra , Takahashi Jacqueline Aparecida
Published: November 2023

Cocoa honey, a by-product obtained during the processing of cocoa, is a juice rich in pectin, organic acids, minerals and phenolic compounds with antioxidant properties. Fresh cocoa honey is quickly fermented due to its high content of reducing sugars, such as fructose and glucose, which limits its shelf life. Currently, cocoa honey is only commercialized in frozen form, as logistical challenges prevent the wide distribution or export of this by-product for applications in the market of sweets, jellies, beverages, confectionery, and nutraceutical foods among others. Spray-drying technology is a viable prospect for the large-scale stabilization of products such as cocoa honey, with less heat exposure compared to other conventional drying methods. This work aimed to evaluate the efficacy of drying adjuvants for a rapid removal of the water present in cocoa honey via atomization, since this process minimizes the effects of glass transition temperature (Tg) related to materials with high sugar contents. Physical parameters such as the moisture content, hygroscopicity, particle size, and yield of the products obtained were determined. Cocoa honey presented 85.3 ± 0.20 g/100 g of moisture. The formulations successfully decreased moisture content, which was lower than 11.72 ± 0.08 g/100 g in the formulations. Water activity ranged between 0.1464 ± 0.0043 and 0.1562 ± 0.029, with no significant difference between the formulations. The hygroscopicity of cocoa honey powders ranged from 29.29 to 29.87 g of water/100 g of cocoa honey. The combination of 20% maltodextrin and 1% whey protein isolate (WPI) led to the best yield, resulting in a free-flowing powder as the final product. On the other hand, the formulation composed of maltodextrin and whey protein isolate in the ratio of 29:1, respectively, led to the most stable product, with less loss of phenolic compounds during the drying process (6.04%). Regarding particle diameter, 90% of the accumulated distribution did not exceed 57 μm. The greatest dispersion of particles occurs in the Ma20W10 formulation with a span of 2.72, inferring greater variation in size between small (7.01 ± 0.06 μm), medium (18.25 ± 0.37 μm), and large (56.65 ± 1.17 μm) particles. The use of whey protein isolate as an adjuvant proved to be an efficient drying process in the production of cocoa honey powder, and was also advantageous for enriching the nutritional content of the final product due to its protein origin. Furthermore, the combination of spray-drying technology and the use of whey protein isolate as adjuvant led to a free-flowing cocoa honey powder with an adequate particle size and benefits in terms of shelf-life extension, providing new opportunities for the commercialization of cocoa honey as an ingredient for the food industry, with benefits for the circular economy.
Influence of Geographical Origin on Isotopic and Elemental Compositions of Pork Meat
Authors: Dehelean Adriana , Feher Ioana , Romulus Puscas , Magdas Dana Alina , Covaciu Florina-Dorina , Kasza Angela Maria , Curean Victor , Cristea Gabriela
Published: November 2023

Pigs are a primary source of meat, accounting for over 30% of global consumption. Consumers’ preferences are determined by health considerations, paying more attention to foodstuffs quality, animal welfare, place of origin, and swine feeding regime, and being willing to pay a higher price for a product from a certain geographical region. In this study, the isotopic fingerprints (δ2H, δ18O, and δ13C) and 29 elements of loin pork meat samples were corroborated with chemometric methods to obtain the most important variables that could classify the samples’ geographical origin. δ2H and δ18O values ranged from −71.0 to −21.2‰, and from −9.3 to −2.8‰, respectively. The contents of macro- and micro-essential elements are presented in the following order: K > Na > Mg > Ca > Zn > Fe > Cu > Cr. The LDA model assigned in the initial classification showed 91.4% separation of samples, while for the cross-validation procedure, a percentage of 90% was obtained. δ2H, K, Rb, and Pd were identified as the most representative parameters to differentiate the pork meat samples coming from Romania vs. those from abroad. The mean values of metal concentrations were used to estimate the potential health risks associated with the consumption of pork meat The results showed that none of the analyzed metals (As, Cd, Sn, Pb, Cu, and Zn) pose a carcinogenic risk.
Microbial Quality of Donkey Milk during Lactation Stages
Authors: Zhou Miaomiao , Huang Fei , Du Xinyi , Wang Changfa , Liu Guiqin
Published: November 2023

The microbial community in donkey milk and its impact on the nutritional value of donkey milk are still unclear. We evaluated the effects of different lactation stages on the composition and function of donkey milk microbiota. The milk samples were collected at 1, 30, 60, 90, 120, 150, and 180 days post-delivery. The result showed that the microbial composition and functions in donkey milk were significantly affected by different lactation stages. The dominant bacterial phyla in donkey milk are Proteobacteria (60%) and Firmicutes (22%). Ralstonia (39%), Pseudomonas (4%), and Acinetobacter (2%) were the predominant bacterial genera detected in all milk samples. In the mature milk, the abundance of lactic acid bacteria Streptococcus (7%) was higher. Chloroplast (5%) and Rothia (3%) were more plentiful in milk samples from middle and later lactation stages (90–180 d). Furthermore, the pathogens Escherichia-Shigella and Staphylococcus and thermoduric bacteria Corynebacterium, Arthrobacter, and Microbacterium were also detected. Donkey milk is rich in beneficial bacteria and also poses a potential health risk. The above findings have improved our understanding of the composition and function changes of donkey milk microbiota, which is beneficial for the rational utilization of donkey milk.
Recent Advances in and Applications of Electrochemical Sensors Based on Covalent Organic Frameworks for Food Safety Analysis
Authors: Zhu Hongwei , Li Minjie , Cheng Cuilin , Han Ying , Fu Shiyao , Li Ruiling , Cao Gaofeng , Liu Miaomiao , Cui Can , Liu Jia , Yang Xin
Published: November 2023

The international community has been paying close attention to the issue of food safety as a matter of public health. The presence of a wide range of contaminants in food poses a significant threat to human health, making it vital to develop detection methods for monitoring these chemical contaminants. Electrochemical sensors using emerging materials have been widely employed to detect food-derived contaminants. Covalent organic frameworks (COFs) have the potential for extensive applications due to their unique structure, high surface area, and tunable pore sizes. The review summarizes and explores recent advances in electrochemical sensors modified with COFs for detecting pesticides, antibiotics, heavy metal ions, and other food contaminants. Furthermore, future challenges and possible solutions will be discussed regarding food safety analysis using COFs.
Effect of Ultrasound and Salt on Structural and Physical Properties of Sodium Alginate/Soy Protein Isolates Composite Fiber
Authors: Zeng Xinyue , Cui Bing , Zhou Bin , Liang Hongshan , Wu Di , Li Jing , Li Bin
Published: November 2023

Recently, there has been a growing interest in advancing plant-based or cultured meat substitutes as environmentally and ethically superior alternatives to traditional animal-derived meat. In pursuit of simulating the authentic meat structure, a composite fiber composed primarily of soy protein isolates (SPIs) was fashioned, employing a fiber-based plant-based analog meat construct. To refine the spinning process and enhance fiber quality, we employed ultrasound treatment, a physical modification technique, to scrutinize its influence on SPI protein structure. This inquiry extended to the examination of the interplay between sodium alginate (SA) and SPI, as well as the impact of salt ions on the SA and ultrasound soy protein isolates (USPI) interaction. A comprehensive exploration encompassing ultrasound treatments and salt concentrations within the composite solution, along with their repercussions on composite fiber characterization, with a rise in negative zeta potential value, states the ultrasound treatment fosters protein aggregation. Moreover, the introduction of salt augments protein aggregation as salt content escalates, ultimately resulting in a reduced structural viscosity index and improved spinnability. The presence of Ca2+ ions during the coagulation process leads to interactions with SA. The involvement of ultrasound prompts the exposure of hydrophilic amino acid segments in the protein to water, leading to the development of a more porous structure. Solely under the influence of ultrasound, the fiber exhibits 5% higher water-holding capacity and superior mechanical properties while maintaining comparable thermal stability.
Antioxidant Effects and Probiotic Properties of Latilactobacillus sakei MS103 Isolated from Sweet Pickled Garlic
Authors: Li Heng , Chen Changlin , Li Yuanxin , Li Zhengqiang , Li Chen , Luan Chang
Published: November 2023

Fermented vegetable-based foods, renowned for their unique flavors and human health benefits, contain probiotic organisms with reported in vitro antioxidative effects. This study investigates the probiotic properties of Latilactobacillus sakei MS103 (L. sakei MS103) and its antioxidant activities using an in vitro oxidative stress model based on the hydrogen peroxide (H2O2)-induced oxidative damage of RAW 264.7 cells. L. sakei MS103 exhibited tolerance to extreme conditions (bile salts, low pH, lysozyme, H2O2), antibiotic sensitivity, and auto-aggregation ability. Moreover, L. sakei MS103 co-aggregated with pathogenic Porphyromonas gingivalis cells, inhibited P. gingivalis-induced biofilm formation, and exhibited robust hydrophobic and electrostatic properties that enabled it to strongly bind to gingival epithelial cells and HT-29 cells for enhanced antioxidant effects. Additionally, L. sakei MS103 exhibited other antioxidant properties, including ion-chelating capability and the ability to effectively scavenge superoxide anion free radicals, hydroxyl, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid, and 2,2-diphenyl-1-picrylhydrazyl. Furthermore, the addition of live or heat-killed L. sakei MS103 cells to H2O2-exposed RAW 264.7 cells alleviated oxidative stress, as reflected by reduced malondialdehyde levels, increased glutathione levels, and the up-regulated expression of four antioxidant-related genes (gshR2, gshR4, Gpx, and npx). These findings highlight L. sakei MS103 as a potential probiotic capable of inhibiting activities of P. gingivalis pathogenic bacteria and mitigating oxidative stress.
Nisin and Organic Acid Salts Improved the Microbial Quality, Extended the Shelf Life, and Maintained the Sensory Attributes of Semidry Beef Luncheon Marketed at Adverse (35–40 °C) Ambient Summer Temperatures
Authors: Elbanna Ahmed Medhat , Sabala Rana Fahmi , Abd-Elghany Samir Mohammed , Imre Kálmán , Morar Adriana , Herman Viorel , Sallam Khalid Ibrahim
Published: November 2023

Semidry beef luncheon may undergo deteriorative changes during storage at ambient temperatures in tropical and subtropical regions including Egypt. This study was conducted in a meat plant in Egypt with the aim of overcoming the economic losses from the returns of spoiled unsold beef luncheon displayed in grocery stores at adverse summer temperatures of 37 °C or more. Ten approaches were applied using different preservatives, comprising sodium nitrite, nisin, potassium sorbate, and organic acid salts (a combination of sodium lactate, sodium acetate, and sodium diacetate). In addition, the product was cooked at different temperatures and was stored for 21 days at 37 °C, during which time the shelf life, microbial quality, pH, and sensory attributes were investigated. By Day 21 of storage, the luncheon contained 50 mg/kg sodium nitrite, 25 mg/kg nisin, and 1000 mg/kg organic acid salts and, when cooked at a final core temperature of 92 °C, exhibited reductions in aerobic plate count, anaerobic plate count, lactic acid bacterial count, and mold and yeast counts by 4.32, 3.54, 3.47, and 1.89 log10 CFU/g, respectively, when compared with the control. The sensory attributes and pH were also maintained in the final products of such treatment, with no product return and the avoidance of economic loss. This study presents a novel approach for solving the major problem of the deteriorative changes that occur in semidry luncheon sausage and similar meat products which require rejection with a huge economic loss, especially in tropical and semitropical areas of the world that have similar problems of high climatic temperatures and a low availability of energy or technological resources.
Effects of Counterion on the Formation and Hydration Behavior of α-Form Hydrated Crystals (α-Gels)
Authors: Sakai Kenichi , Nishimoto Shuri , Hirai Yuki , Arakawa Kyosuke , Akamatsu Masaaki , Tanaka Keisuke , Suzuki Toshiyuki , Sakai Hideki
Published: November 2023

α-Form hydrated crystals form a lamellar gel in which the alkyl chains of the amphiphilic molecules are hexagonally arranged within bilayers below the gel–liquid crystal phase transition temperature. In practice, the lamellar gel network with excess water is called an “α-gel”, particularly in the cosmetics industry. In this study, the hydration or water sorption of amphiphilic materials in water vapor was assessed using a humidity-controlled quartz crystal microbalance with dissipation monitoring (QCM-D) technique. The amphiphilic materials used in this study were hexadecyl phosphate salts neutralized with L-arginine (C16P-Arg), CsOH (C16P-Cs), KOH (C16P-K), and NaOH (C16P-Na). Small- and wide-angle X-ray scattering measurements revealed that C16P-Arg and C16P-Cs yielded α-form hydrated crystals. Humidity-controlled QCM-D measurements demonstrated that C16P-Arg and C16P-Cs more readily underwent hydration or water sorption than C16P-K and C16P-Na. The key conclusion is that the significant hydration ability of C16P-Arg and C16P-Cs promotes the formation of the corresponding α-form hydrated crystals.
The Contribution of Digital Image Correlation for the Knowledge, Control and Emergency Monitoring of Earth Flows
Authors: Mazza Davide , Romeo Saverio , Cosentino Antonio , Mazzanti Paolo , Guadagno Francesco Maria , Revellino Paola
Published: November 2023

Earth flows are complex geological processes that, when interacting with linear infrastructures, have the potential to cause significant damage. Consequently, conducting comprehensive investigations and continuous monitoring are becoming paramount in designing effective mitigation measures and ensuring sustainable risk management practices. The use of digital image correlation (DIC) algorithms for the displacement monitoring of earth flows is a relatively new and growing field with a limited number of dedicated works in the literature. Within this framework, the present paper contributes by evaluating DIC techniques at several earth flow sites. These assessments were founded upon data obtained from both low-cost and multi-platform sensors, used to assess the displacement field, pinpointing the most active sectors, and gauging their rates of movement during different kinematic phases. In this regard, the highest localized movement velocities reached in the Pietrafitta (0.01 m/day) and Grillo (0.63 m/day) earth flows experience different ongoing kinematic processes. The versatility of these techniques is notable, manifesting in the ease of installation, cost-effectiveness, and adaptability to various platforms. The proven reliability in terms of maximum accuracy (1 pixel) and sensitivity (0.05 m) lays the foundation for the extensive use of the presented techniques. The amalgamation of these attributes positions DIC techniques as competitive, adaptable, and readily deployable tools in earth flow studies.
Healthcare Service Quality Evaluated Using the Degree of Satisfaction of Patients in Romanian Community Pharmacies
Authors: Bîrsan Magdalena , Focșa Alin-Viorel , Cristofor Ana Caterina , Scripcariu Sadiye-Ioana , Antonoaea Paula , Vlad Robert-Alexandru , Todoran Nicoleta , Ciurba Adriana , Stan Cristinel Ionel , Apostol Manuela Maria , Stan Catalina Daniela
Published: November 2023

Community pharmacy has evolved a lot in recent years in terms of pharmaceutical services and marketing policies applied in Romania. This study aimed to evaluate the degree of patient satisfaction in community pharmacies in Iași, Romania correlated with the frequency of returning to the pharmacy, level of education, gender, and stress level at the time when the pharmacist dispenses the medication. A total of 30 community pharmacies were involved, and in a period of three months, they issued questionnaires to patients. 722 patients responded, and to verify the first research hypothesis, the Pearson correlation was applied. Statistical analysis revealed that there is a negative, medium-level, and significant correlation between the level of satisfaction with pharmaceutical services and the frequency of visits to the pharmacy, r = −0.342, p < 0.0001. There is also a significant, negative correlation of low intensity between the level of satisfaction with pharmaceutical services and patient status, r = −0.202, p < 0.0001. The degree of patient satisfaction is influenced by the quality of the basic pharmaceutical service offered, by the frequency of visits to the pharmacy, by the level of stress, and by social class.
“Always Look at the Clock”: Psychosocial Working Conditions in Outpatient Care—A Qualitative Study
Authors: Bernburg Monika , Harth Volker , Groneberg David A. , Mache Stefanie
Published: November 2023

Background: In view of demographic change, there is a growing need for qualified nursing professionals. More and more people in need of care prefer to be cared for at home. A shortage of skilled workers and constantly changing and increasing professional requirements are some of the consequences. The aim of this study is to generate further insights into work-related psychosocial stress factors and the resources of outpatient caregivers in their subjective perceptions to derive approaches for possible health-promoting interventions for practice and research that promote healthier and more sustainable work. In addition, coping strategies and needs should be inquired about in order to determine support offers. Methods: Using a semi-structured interview guide, thirty outpatient nursing professionals in Germany were interviewed in 2022. The subject of this interview was the psychosocial working conditions and resources in outpatient care. The collected data were evaluated by means of the Kuckartz qualitative content analysis. Results: This qualitative study identified work-related job demands and resources in outpatient care. The way nurses are treated in the companies they work for and the appreciation of patients were explored as important resources. Physical demands, the time factor, and dealing with personal fates are primarily mentioned as stress factors. The learning effect plays an important role in dealing with these. Conclusion: This study contributes to a better understanding of current job strain and resources as well as job satisfaction in outpatient care. In any case, it became clear that the nursing staff love their profession, which they see more as a vocation. Future quantitative studies are necessary to build on these findings. First ideas of implications for health promotion measures in research and practice have already been derived.
Anxiety and Coping Strategies among Italian-Speaking Physicians: A Comparative Analysis of the Contractually Obligated and Voluntary Care of COVID-19 Patients
Authors: Rizzo Amelia , Yıldırım Murat , Aziz Izaddin Ahmad , Çağış Zafer Güney , Szarpak Łukasz , Nucera Gabriella , Sitibondo Aldo , Khabbache Hicham , Chirico Francesco , Nowrouzi-Kia Behdin
Published: November 2023

This study aims to explore the differences in the psychological impact of COVID-19 on physicians, specifically those who volunteered or were contractually obligated to provide care for COVID-19 patients. While previous research has predominantly focused on the physical health consequences and risk of exposure for healthcare workers, limited attention has been given to their work conditions. This sample comprised 300 physicians, with 68.0% of them men (mean age = 54.67 years; SD = 12.44; range: 23–73). Participants completed measurements including the State-Trait Anxiety Inventory (STAI), Coping Inventory in Stressful Situations (CISS), and Coronavirus Anxiety Scale (C.A.S.). Pearson’s correlations were conducted to examine the relationships between the variables of interest. This study employed multivariate models to test the differences between work conditions: (a) involvement in COVID-19 patient care, (b) volunteering for COVID-19 patient management, (c) contractual obligation to care for COVID-19 patients, and (d) COVID-19 contraction in the workplace. The results of the multivariate analysis revealed that direct exposure to COVID-19 patients and contractual obligation to care for them significantly predicted state anxiety and dysfunctional coping strategies [Wilks’ Lambda = 0.917 F = 3.254 p < 0.001]. In contrast, volunteering or being affected by COVID-19 did not emerge as significant predictors for anxiety or dysfunctional coping strategies. The findings emphasize the importance of addressing the psychological well-being of physicians involved in COVID-19 care and highlight the need for targeted interventions to support their mental and occupational health.
Factors Related to Obstructive Sleep Apnea According to Age: A Descriptive Study
Authors: Kwon Myoungjin , Kim Jiyoung , Kim Sun Ae
Published: November 2023

This descriptive study aimed to identify the factors influencing obstructive sleep apnea (OSA) by age between middle-aged and elderly people. These groups have not been evaluated separately until now. This study is a secondary analysis of data from the Eighth Korea National Health and Nutrition Examination Survey 2021. Of the 3942 participants with OSA in this study, 2397 were middle-aged and 1545 were elderly, and 2509 had low risk and 1433 had moderate–high risk. Age-specific factors related to their OSA were identified using complex sample logistic regression. Factors associated with OSA in middle-aged individuals included the number of household members, smoking, subjective health, and subjective body image. Smoking and subjective health were significantly related to OSA in elderly people. Not smoking was 0.23 times less likely than smoking to be associated with OSA, and 0.3 times less likely when participants were healthy than when unhealthy. Hence, influencing factors differed depending on the age of individuals with OSA. Therefore, to develop public health measures, it may be more effective to establish intervention strategies to improve symptoms and prevent complications in middle-aged and elderly patients with OSA by distinguishing and applying influential age-specific factors.
Citation Network Analysis of Nurse Staffing Research from the Past Two Decades: 2000–2022
Authors: Morioka Noriko , Ochi Masanao , Okubo Suguru , Moriwaki Mutsuko , Hayashida Kenshi , Sakata Ichiro , Kashiwagi Masayo
Published: November 2023

Studies have indicated that higher numbers of nurses regarding staffing ensure patient safety and a better practice environment. Using citation analysis, this study visualizes the landscape of nurse staffing research over the last two decades to show the overall publication trends, major contributors, and main research topics. We extracted bibliometric information from PubMed from January 2000 to September 2022. After clustering the network, we analyzed each cluster’s characteristics by keyword. A total of 2167 papers were considered for analysis, and 14 clusters were created. The analysis showed that the number of papers published per year has been increasing. Researchers from the US, the UK, Canada, Australia, and Belgium have led this field. As the main clusters in nurse staffing research during the past two decades, the following five research settings were identified: nurse outcome and patient outcome research in acute care hospitals, nurse staffing mandate evaluation research, nursing home research, and school nurse research. The first three clusters accounted for more than 80% of the total number of published papers, and this ratio has not changed in the past 20 years. To further develop nurse staffing research globally, evidence from other geographic areas, such as African and Asian countries, and from long-term care or community settings is necessary.
Awareness and Sources of Knowledge about Obstructive Sleep Apnea: A Cross Sectional Survey Study
Authors: Bartolucci Maria Lavinia , Incerti Parenti Serena , Bortolotti Francesco , Gorini Tommaso , Alessandri-Bonetti Giulio
Published: November 2023

Obstructive sleep apnea (OSA) is a multifactorial sleep breathing disorder, seriously impacting quality of life and involving approximately 1 billion of the world’s population. It is characterized by episodes of total cessation of breathing or decreases in airflow during sleep. Available data suggest that most cases of OSA remain undiagnosed even in developed countries. This is due to a lack of widespread knowledge about this pathology and the medical morbidities and mortality it brings about, among both laypeople and physicians. Moreover, despite receiving indications about the need to undergo specific evaluations for OSA signs and symptoms, sometimes patients do not pay sufficient attention to the problem. This is probably due to a lack of correct information on these issues. The present investigation analyzed the level of knowledge about OSA pathology and the sources through which a group of OSA patients gained information on their condition. A survey of 92 patients diagnosed with OSA (mean age 60.55 ± 10.10) and referred to the Unit of Orthodontics and Dental Sleep Medicine of the University of Bologna was conducted by means of a questionnaire investigating sociodemographic characteristics, the level of general knowledge on OSA pathology and its possible medical consequences. Despite about two third (67.38%) of the population demonstrating extensive knowledge, remarkably, a group of subjects (20.65%) had poor awareness of the OSA condition. A statistically significant correlation emerged between the level of knowledge about OSA and the level of education (p = 0.002). A great effort should be made to improve the quality of information and the communication modalities for OSA to enable a fully appropriate awareness of the condition among patients.
Germination, Physicochemical Properties, and Antioxidant Enzyme Activities in Kangkong (Ipomoea aquatica Forssk.) Seeds as Affected by Dielectric Barrier Discharge Plasma
Authors: Ongrak Prapasiri , Poolyarat Nopporn , Suksaengpanomrung Suebsak , Saidarasamoot Kamtorn , Jirakiattikul Yaowapha , Rithichai Panumart
Published: November 2023

Dielectric barrier discharge (DBD) plasma has been utilized as a sustainable technology to enhance seed germination in various plant species. The objective of this research was to identify the mechanism of physicochemical properties and antioxidant enzyme activities to promote kangkong (Ipomoea aquatica Forssk.) seed germination using different durations of DBD plasma treatments. Seeds were exposed to atmospheric DBD plasma from 5 to 20 min, compared to non-treated seeds as the control. According to SEM images, the seed surface had cracks and grew wider as a result of the prolonged DBD plasma treatments. A longer DBD plasma treatment exhibited a lower water contact angle and increased water absorption. DBD plasma treatments strongly improved germination percentages and hydrogen peroxide (H2O2) contents. Seeds treated with DBD plasma for 20 min showed the highest malondialdehyde (MDA) content and the lowest field emergence. Catalase (CAT) activity increased under DBD plasma treatments for 5 and 10 min. Ascorbate peroxidase (APX) and superoxide dismutase (SOD) activities were not statistically different among the treatments. This finding suggested that DBD plasma treatments stimulated the germination of kangkong seeds by modifying the seed surface, and upregulating H2O2 content and CAT activity. Five minutes was an appropriate time to treat DBD plasma.
Evolution of Seed Dispersal Modes in the Orchidaceae: Has the Vanilla Mystery Been Solved?
Authors: Karremans Adam P. , Watteyn Charlotte , Scaccabarozzi Daniela , Pérez-Escobar Oscar A. , Bogarín Diego
Published: November 2023

Orchid seeds are predominantly wind-dispersed, often developed within dry, dehiscent fruits that typically release millions of dust-like seeds into the air. Animal-mediated seed dispersal is a lesser-known phenomenon in the family and predominantly occurs in groups belonging to early-diverging lineages bearing indehiscent, fleshy fruits with hard, rounded, dark seeds. In this review, we explore the evolutionary trends of seed dispersal mechanisms in Orchidaceae, focusing on the pantropical genus Vanilla. Notably, certain Neotropical species of Vanilla produce vanillin-aromatic compounds synthesized naturally in their fruits, which plays a pivotal role in seed dispersal. Ectozoochory occurs in dry, dehiscent fruits, whose seeds are dispersed by (i) male euglossine bees collecting the fruit’s vanillin aromatic compounds and (ii) female stingless bees collecting the fruit’s mesocarp. Endozoochory occurs in (iii) highly nutritious, indehiscent fruits consumed by terrestrial mammals or (iv) fleshy, dehiscent fruits whose mesocarp is consumed by arboreal mammals. Wind dispersal appears to be a derived state in Orchidaceae and, given its predominance, a trait likely associated with enhanced speciation rates. Zoochory primarily occurs in groups derived from early-diverging lineages; occasional reversions suggest a link between dispersal mode and fruit and seed traits. Interestingly, fruit dehiscence and fleshiness in Vanilla lack phylogenetic signal despite their role in determining dispersal modes, suggesting potential environmental adaptability.
Spatial Accessibility of Public Electric Vehicle Charging Services in China
Authors: Chen Yu , Chen Yuehong , Lu Yuqi
Published: November 2023

Decarbonizing the transport sector using electric vehicles (EVs) is a vital pathway for China to achieve the carbon peak and carbon neutrality goals. Despite the unprecedented growth of EV diffusion in China, little information is available for the spatial accessibility of public electric vehicle charging services (EVCSs). This study developed an applicable accessibility measurement framework to examine the city-level accessibility of EVCSs in China using the Gaussian two-step floating catchment area (G2SFCA) method. G2SFCA takes the EV charging stations with charging piles as supply and the EV ownership data as demand. The results indicate that (1) the eastern region of China has the highest density of EV charging stations (69.1%), followed by the central region, while the western region has the lowest density; (2) the spatial accessibility of EVCSs has a different pattern, where the central region has the highest accessibility, followed by the eastern and western regions; (3) the spatial mismatch between EVCSs and EV diffusion in the eastern region is larger than that of the other two regions, which may be attributed to the suboptimal layout of EV charging stations and the inconsistent pace between EV penetration and EV charging station construction; and (4) there is a significant spatial inequity in the accessibility of EVCSs across both all three regions and the entirety of China, with the western region exhibiting the highest inequity, followed by the central and eastern regions. Based on these findings, policy implications are drawn for different regions in China, which may aid policymakers in crafting strategic policies and subsidy programs to foster the advancement of EVCSs.