bluuebunny/crossref_metadata_2025 huggingface dataset:
Improved Safety of Hybrid Electroconvulsive Therapy Compared With Standard Electroconvulsive Therapy in Patients With Major Depressive Disorder: A Randomized, Double-Blind, Parallel-Group Pilot Trial
Authors: Zhang Jing-ya , Xu Shu-xian , Zeng Lun , Chen Li-chang , Li Jia , Jiang Zhao-yun , Tan Bai-jian , Gu Chen-long , Lai Wen-tao , Kong Xiao-ming , Wang Jian , Rong Han , Xie Xin-hui
Published: May 2022

Objectives

ECT is a rapid and effective treatment for depression. While efficacy is often remarkable over the initial 3–4 sessions, the efficacy of later sessions is less rapid, and the side-effects, especially cognitive impairment limit its use. To preliminarily compare the efficacy and acceptability of a novel hybrid-ECT (HECT) protocol for patients with major depressive disorder (MDD) with standard ECT, we conducted this pilot trial.

Methods

Thirty patients were randomly assigned to ECT or HECT. Both arms received three ECT sessions (phase 1) but, in phase 2, the HECT arm received low-charge electrotherapy instead of ECT. The primary outcome was the change in 24-item Hamilton depression rating scale (HAMD-24) scores between baseline and the end of treatment. Cognitive function was assessed by repeatable battery for the assessment of neuropsychological status (RBANS), Stroop color word, and orientation recovery tests (ORT). Safety was measured by the drop-out rate and adverse events (AEs). Four visits were conducted at baseline, post-phase 1, post-phase 2, and at 1-month follow-up. Trial registration: Chinese Clinical Trial Registry (http://www.chictr.org.cn/), identifier: ChiCTR1900027701.

Results

Patients in both arms showed significant within-group improvements in HAMD-24, but the between-group differences were non-significant. Participants in the HECT arm outperformed ECT patients for most cognitive tests at the end of treatment or at follow-up. There was a significantly lower AE rate and shorter ORT in phase 2 of the HECT ar.

Conclusion

In this pilot trial, HECT was associated with fewer AEs and better cognitive function including executive and memory function, but its possible similar antidepressive efficacy needs to be further investigated in future.
Effectiveness of a Parent-Focused Intervention Targeting 24-H Movement Behaviors in Preschool-Aged Children: Study Protocol for a Randomized Controlled Trial
Authors: Feng Jie , Huang Wendy Yajun , Sit Cindy Hui-Ping
Published: May 2022

Background

Interventions targeting single behaviors of preschool-aged children have been mainstream for some time, but integrated interventions targeting all three 24-h movement behaviors (physical activity [PA], sedentary behavior [SB], and sleep hygiene) are less studied. The aims of this study will be to test the feasibility, acceptability, and effectiveness of a parent-focused intervention for preschool-aged children targeting multiple 24-h movement behaviors.

Methods

This three-arm randomized controlled trial will comprise a 12-week intervention and a 12-week follow-up. A total of 150 parent-child dyads will be recruited and randomly allocated to one of three arms: (1) a PA + SB group (dyadic approach), (2) a PA + SB + sleep group (integrated approach), and (3) a wait-list control group. The theory of planned behavior and behavioral change techniques will guide the development of the intervention via workshops, education materials, interactive questionnaires, and reminders. The intervention strategies for the integrated group will be the same as for the dyadic approach except that the intervention will also target sleep hygiene in addition to PA and SB. The primary outcomes will be preschoolers' 24-h movement behaviors (e.g., activity sleep index, compositional data of PA, SB, screen time, and sleep duration). The secondary outcomes will be preschoolers' sleep quality, weight status, cognitive function, and parents' movement behaviors. The feasibility and acceptability of the intervention will also be evaluated.

Discussion

The proposed study will be a theory-based, parent-focused intervention designed to improve all three 24-h movement behaviors among preschoolers. The trial is expected to improve preschoolers' movement behaviors and health outcomes, as well as their parents' movement behaviors. Given the urgent need to promote active lifestyles, our findings will help to determine best practices for movement behavior change among young children.

Trial Registration

The study is prospectively registered at the Chinese Clinical Trial Registry (ChiCTR2200055958).
Nutritional Characterization of Street Food in Urban Turkmenistan, Central Asia
Authors: Albuquerque Gabriela , Sousa Sofia , Lança de Morais Inês , Gelormini Marcello , Motta Carla , Gonzales Gerard Bryan , Ovezov Azat , Damasceno Albertino , Moreira Pedro , Breda João , Lunet Nuno , Padrão Patrícia
Published: May 2022

Objective

Describing the availability and nutritional composition of the most commonly available street foods in Ashgabat, Turkmenistan.

Methods

One hundred sixty-one street food vending sites (six public markets) were assessed, through a collection of data on vending sites' characteristics and food availability, and samples of commonly available foods (21 homemade; 11 industrial), for chemical analysis.

Results

Fruit, beverages, and food other than fruit were available in 6.8, 29.2, and 91.9% of all vending sites, respectively. Regarding the latter, 52.7% of the vending sites sold only homemade products (main dishes, snacks, cakes, biscuits and pastries, bread, ice-cream chocolate and confectionery, savory pastries and sandwiches), 37.2% only industrial (ice-cream, chocolate and confectionery, cakes, biscuits and pastries, snacks, bread and savory pastries) and 10.1% both. Homemade foods presented significantly higher total fat [homemade 11.6 g (range 6.6–19.4 g); industrial 6.2 g (range 4.0–8.6 g), p = 0.001], monounsaturated, polyunsaturated and trans-fat, and sodium and potassium content per serving. Industrial wafers presented the highest mean saturated (11.8 g/serving) and trans-fat (2.32 g/serving) content. Homemade hamburgers presented the highest mean sodium content (1889 mg/serving).

Conclusions

Strategies to encourage the production and sales of healthier street foods, especially homemade, are needed to promote healthier urban food environments in urban Turkmenistan.
Transforming Perspectives Through Virtual Exchange: A US-Egypt Partnership Part 2
Authors: Collins Sarah L. , Mueller Savanah , Wood Elizabeth A. , Stetten Nichole E.
Published: May 2022

Collaborative online international learning programs, such as virtual exchange, that utilize telecollaborative activities have been integrated into more classrooms within the higher education setting. These programs provide students exposure to international cultures, perspectives, and ideas is no longer considered “value added”, but a prerequisite to entering many workforces. These programmatic objectives compliment Mezirow's Transformative Learning Theory, that substantiates two major elements of transformative learning are critical reflection and dialectical discourse. This study presents the second half of a qualitative inquiry into the prominent themes that arose during a virtual exchange that was conducted in March 2021 between students in the United States (US) enrolled in a global public health course and Egyptian microbiology students. This study sought to expand upon the Transformative Learning Theory through inductive analysis procedures to offer a modernized adaptation of the theoretical framework within international learning environments. Student responses enrolled in an undergraduate global public health course were collected and analyzed by two coders using inductive/open coding to identify salient codes. These codes were then summarized into categories and subsequently defined. Resulting themes include Connectedness, Openness, Acquisition of Knowledge and Skills, Communication, Cultural Identity, Anticipation of Options for New Roles, Relationships, and Actions, and Absence of Change. Several themes have corresponding categories and subcategories. Adult learning environments such as the modern college classroom have changed with the introduction and reliance upon online learning domains, as well as the diversification of higher education student demographics, accentuating the need to inductively analyze student learning processes and outcomes. In doing so, our findings provide a modernized adaptation of the Transformative Learning Theory that allows for adult learning theorists, researchers, and scholars to integrate tenets of transformative learning more appropriately. As such, this provides an opportunity for educators to coalesce the identified mechanisms (e.g., openness, cultural background, anticipation of roles and relationships) to bolster student's willingness and ability to engage in transformative critical reflections. By capitalizing on students' innate characteristics, such as open-mindedness predispositions and cultural background, educators are able to augment transformative learning strategies through tailored assignments and course activities.
The Relationship Between Adjustment and Mental Health of Chinese Freshmen: The Mediating Effect of Security and the Moderating Effect of Gender
Authors: Cao Liu
Published: May 2022

During the COVID-19 pandemic, many universities are confronted with campus lockdown or even school closures to reduce the risk of infection. However, these measures pose a threat to the mental health of adolescents. In particular, freshmen who have just entered the university campus may suffer from more serious mental health risks. In this study, 1,818 freshmen were analyzed by using the Chinese College Student Adjustment Scale (CCSAS), Sense of Security Questionnaire (SQ), and Symptom Check List 90 (SCL-90) of the qualitative method. The results showed that adjustment had an impact on mental health. Firstly, there was a significant negative association between adjustment and mental health. Secondly, adjustment had a significant predictive effect on mental health. Finally, a sense of security and gender affected the relationship between adjustment and mental health. Collectively, adjustment, sense of security, and gender exerted impacts on the mental health of freshmen, suggesting that we should create a warm and harmonious campus environment for students and conduct targeted education for male and female students.
Serious Games: A new Approach to Foster Information and Practices About Covid-19?
Authors: Montalbano L. , Gallo L. , Ferrante G. , Malizia V. , Cilluffo G. , Fasola S. , Alesi M. , La Grutta S.
Published: May 2022

The current Covid-19 pandemic poses an unprecedented global challenge in the field of education and training. As we have seen, the lack of proper information about the virus and its transmission has forced the general population and healthcare workers to rapidly acquire knowledge and learn new practices. Clearly, a well-informed population is more likely to adopt the correct precautionary measures, thus reducing the transmission of the infection; likewise, properly educated healthcare workers are better equipped to manage the emergency. However, the need to maintain physical distancing has made it impossible to provide in-presence information and training. In this regard, new technologies have proved to be an invaluable resource by facilitating distance learning. Indeed, e-learning offers significant advantages because it does not require the physical presence of learners and teachers. This innovative method applied to serious games has been considered potentially effective in enabling rapid and large-scale dissemination of information and learning through content interactivity. We will review studies that have observed the development and use of serious games to foster information and practices about Covid-19 aimed at promoting behavioral changes in the population and the healthcare personnel involved on the front line.
All Athletes Can Lead in Their Own Way
Authors: Loughead Todd M. , Sheppard Mason B. , Hirsch Katherine E.
Published: May 2022

Did you know that not just coaches can be leaders on sport teams? Athletes are also an important source of leadership within teams. When you think of athletes performing leadership roles, you probably think of captains or assistant captains. While these are important sources of team leadership, athletes do not need to be captains or assistant captains to be leaders. In fact, all athletes can display leadership through their behaviors. Coaches can help athletes to be a part of team leadership. We provide some suggestions on how coaches can facilitate the development of leadership skills in their athletes. If athletes are not comfortable being team leaders, they can provide leadership by mentoring fellow teammates. There are many ways that athletes can provide leadership to their teams!
The Vulnerability-Stress-Model—Holding Up the Construct of the Faulty Individual in the Light of Challenges to the Medical Model of Mental Distress
Authors: Demke Elena
Published: May 2022

In the late 1970s, the course seemed to be set for a reconciliation of the controversy around the somatic vs. the social nature of mental distress. The biopsychosocial model and the vulnerability-stress-model were influential agents in this move, but a medicalized somatic view on mental distress persisted nonetheless. The reasons for this persistence are complex, and naturally include questions of structural power. However, the adherence to a certain fundamental framing of a problem may continue to be transmitted not only out of conviction, but also unwittingly. The vulnerability-stress-model allowed those who used it to effectively stick to the implications of a medicalized somatic view of the faulty individual who falls ill, while also allowing them to believe they integrated the social dimensions of the problem. A close reading and hermeneutical interpretation of the text by Zubin and Spring (1977) and an analysis of its use in psychoeducation serve as a case study in this respect. The vulnerability-stress-model (simply called “vulnerability model” by Zubin and Spring; more often “stress-vulnerability model” by English speaking recipients, and “vulnerability-stress-model” by German authors) seems to have been a success story: since its publication by Zubin and Spring (1977), it has been the point of reference for numerous scholarly and popular (“psychoeducational”) adaptations. It was soon extended from the diagnosis of schizophrenia to various psychiatric diagnoses, understanding mental distress as the result of a trait/state-interaction in the shape of “deviant coping patterns” (Zubin and Spring, p. 112). Recipients appraised the integration of environmental and dispositional factors, some of them opposing the supposed originally integrative intention of the VSM to reduced applications of it (Schmidt, 2012). However, it can be argued that this integration is a matter of rhetorics rather than argumentative essence. Their argument which significantly depends on the use of metaphors, as well as their referencing amounts to a confirmation of a medicalized view on mental distress and a dismissal of the role played by societal factors. Applied to psychoeducation, this paradoxical combination reinforced a view of the persons in question as individually vulnerable, rather than socially wounded. The consequences in terms of what appears as remedy are significant and contribute to turning individual difference into disability.
The FIFA World Cup Qatar 2022 Sustainability Strategy: Human Rights Governance in the Tripartite Network
Authors: O'Rourke Andrew , Theodoraki Eleni
Published: May 2022

The Fédération Internationale de Football Association (FIFA) has faced constant accusations of human rights violations associated with World Cup Qatar 2022, with prominent media coverage and international football team demonstrations. This study aims to analyze and discuss the approach taken by the tripartite policy network of actors, namely FIFA, Qatar's Supreme Council (SC), and the Local Organizing Committee (Q22) for the creation of the the FIFA World Cup Qatar 2022 Sustainability Strategy (hereafter WCSS22) published in January 2020. The WCSS22 represents the first time FIFA has clearly articulated its responsibility in connection with impacts that are linked to the construction and operation of World Cup stadia and facilities, in line with the United Nations Guiding Principles on Business and Human Rights (UNGPs). The strategy was also the first to be planned and delivered jointly by FIFA, the SC, and the Q22. Qualitative Content Analysis (QCA) of documents associated with the WCSS22 was performed to answer the following research questions: a) What are the recurrent features of the policy formulation and design process and what role do the UNGPs play? b) How do FIFA and the other policy actors contribute and position themselves in relation to human rights? c) What form did governance (interdependence, interactions, regulated rules, and steering) take in the policymaking process? The study establishes that there are four recurrent features of policy formulation and design: 1) a collective, systematic, and diverse policymaking approach, 2) emphasis on leveraging internal resources and external input, 3) the building foundation of best practice principles, guidelines, strategies, and existing initiatives, and 4) inconsistency on decision-making and accountability measures. FIFA contributes to policymaking primarily through their existing statutes, human rights policy, and commitments to mitigate negative human rights impacts. Furthermore, specified actions, and mechanisms for construction workers' living and working conditions and recruitment processes are articulated by the SC, who take a more prominent role in worker initiatives. Q22, although involved in collective action, and participating in workers welfare, takes a more peripheral role in the policymaking process. In conclusion, it was found that the tripartite policy network of actors represents a participant-based governance approach with cohesive policy formulation, varied resources at their disposal, inconsistencies in accountability measures and with the lead network role dependent on specific actor initiatives and commitments.
Farmer Perceptions Related to Potato Production and Late Blight Management in Two Communities in the Peruvian Andes
Authors: Perez Willmer , Forbes Gregory Allan , Arias Rolando , Pradel Willy , Kawarazuka Nozomi , Andrade-Piedra Jorge
Published: May 2022

Potato late blight (LB), caused by the oomycete, Phytophthora infestans, continues to be a major constraint of potato in the Andean region and worldwide. Farmers perception regarding potato production with particular emphasis on management practices for LB was surveyed in two communities in the Peruvian highlands using a questionnaire with 25 questions, many with multiple choice questions. In the village of Paucartambo, 84 women and 485 men of different ages participated in the survey, while in the village of Ulcumayo, there were 146 women and 396 men, also of different ages. Overall, responses were similar between the two locations, with some small but significant differences. In general, there was a very clear association between age and education, with only young men and women having secondary education. Respondents in both locations overwhelmingly chose LB as the main constraint to production and cited either personal experience or agrochemical vendors as the primary sources of information about disease management. Many cultivars (25+) are grown in both locations, with the two most important cultivars, Yungay and Huayro, being common in both locations while others differed. There was evidence of confusion around the concept of host plant resistance, as certain cultivars were cited as both relatively resistant and susceptible. Many trade names of fungicides were recalled by respondents, but most were relatively old products. Correspondence analysis indicated gender effects on LB management practices and information sources in both locations that could be taken into consideration to refine future interventions for research and capacity building, such as offering refresher training courses for extension educators, including female extension workers, to help farmers understand basic concepts to manage host resistance and fungicides properly, and improve access to resistant varieties and effective fungicides.
Rhizobacteria of Bali With Obvious Growth-Promoting Properties on Corn (Zea mays L.)
Authors: Maulina Ni Made Intan , Suprapta Dewa Ngurah , Temaja I Gede Rai Maya , Adnyana I Made , Suriani Ni Luh
Published: May 2022

Corn productivity in Indonesia is still relatively low compared with other countries. Therefore, it is necessary to increase the productivity of corn by using rhizobacteria, which have multiple traits. This study was conducted to obtain indigenous rhizobacteria of Bali that have multiple traits, can produce indoleacetic acid (IAA), fix nitrogen from atmosphere, produce siderophores, colonize roots, increase seed germination, and promote the growth of corn. Isolation of rhizobacteria was carried out from the rhizosphere of plants belonging to the Gramineae family that grows in Bali Island, Indonesia. Six isolates, namely, Sr3, Tb9, Rg1, Rg23, Al27, and Jg8, could produce IAA, fix nitrogen from the atmosphere, produce siderophores, and increased germination rate and vigor index of corn seedling. Among them, three isolates, namely, Rg1, Sr3, and Jg8, significantly (p <0.05) increased the germination rate of corn seeds, increased vigor index, increased root dry weight and shoot dry weight of corn at the age of 7 days, and were able to colonize corn roots. Compared with the control, the rhizobacteria treatment increased the germination of corn seeds ranging from 5.04 to 13.05%. Based on the analysis of the 16S rRNA gene, it was found that these rhizobacteria species were Glutamicibacter nicotianae strain Rg1 (accession number OM349119), Brevibacillus invocatus strain Sr3 (accession number OM327515), and Micrococcus luteus strain Jg8 (accession number OM362349). Under a greenhouse condition, all the three isolates significantly (p <0.05) increased nutrient uptake, the leaf chlorophyll content, net assimilation rate, and crop growth rate of corn when compared with control. These results suggested that these isolates of rhizobacteria obviously promoted the growth of corn and can be developed as biostimulant to promote the growth and increase the corn yield in Bali, Indonesia.
Intensity-Modulated Radiotherapy and Three-Dimensional Conformal Radiotherapy Combined with Intracavitary Posterior Radiotherapy for the Treatment of Medium-Term and Advanced Cervical Cancer: Efficacy, Safety and Prognostic Factors
Authors: Yu Kewen , Zhou Liping
Published: May 2022

Objective

To explore the efficacy, safety, and prognostic factors of intensity modulated radiation therapy (IMRT) and three dimensional conformal radiation therapy (3D-CRT) combined with intracavitary posterior radiotherapy for medium-term and advanced cervical cancer.

Methods

Retrospectively analyze the clinical data of 104 patients with medium-term and advanced cervical cancer who were treated in the radiotherapy department of our hospital from September 2015 to March 2017. According to the different radiotherapy techniques, they were divided into the IMRT combined with intracavitary posterior radiotherapy group (n = 52) and the 3D-CRT combined with intracavitary posterior radiotherapy group (n = 52). Observe and compare the short-term efficacy, occurrence of adverse reactions and overall survival rate of the two groups. The clinicopathological characteristics of the survival group and the death group were compared, and univariate analysis and multiple logistic regression models were used to analyze the relationship between the clinicopathological characteristics and the patient’s prognosis.

Results

The total effective rate of IMRT combined with intracavitary posterior radiotherapy group was 96.15%, which was higher than that of 3D-CRT combined with intracavitary posterior radiotherapy group (88.46%), but the difference was not statistically significant (p > 0.05). The incidence of digestive system injury, thrombocytopenia, and radiation proctitis in the IMRT combined intracavitary posterior radiotherapy group was lower than that of the 3D-CRT combined intracavitary posterior radiotherapy group, and the differences were statistically significant (p < 0.05). The prognosis and survival of the two groups of patients were similar, and the difference was not statistically significant (p > 0.05). Pathological classification, clinical stage, and lymph node metastasis are independent influencing factors of 3-year prognosis in patients with medium-term and advanced cervical cancer (p < 0.05).

Conclusion

IMRT combined with intracavitary posterior radiotherapy is equivalent to 3D-CRT combined with intracavitary posterior radiotherapy, but it can reduce the incidence of adverse reactions in patients with medium-term and advanced cervical cancer, and has higher safety. Pathological typing, clinical staging, Lymph node metastasis were independent factor affecting the prognosis of patients. In clinical treatment, IMRT combined with intracavitary posterior radiotherapy is more recommended as a treatment plan for patients with medium-term and advanced cervical cancer.
Identification and Pathogenicity Analysis of the Pathogen Causing Spotted Spleen in Muscovy Duck
Authors: Ke Tianqiao , Yang Dehong , Yan Zhuanqiang , Yin Lijuan , Shen Hanqin , Luo Cuifen , Xu Jingyu , Zhou Qingfeng , Wei Xiaona , Chen Feng
Published: May 2022

Since September 2020, the clinical symptoms of Muscovy duck spleen spots have appeared in Guangdong, Guangxi, Jiangxi, Hunan, Hubei, and other provinces, resulting in a large number of Muscovy duck deaths and great economic losses. The absence of the typical clinical symptoms caused by pathogenic microorganisms makes the cause of the spotted spleen a mystery. High-throughput sequencing results suggested that Riemerella anatipestifer (R. anatipestifer) may be the pathogen. Then, R. anatipestifer was regarded as the research target for isolation, identification, and pathogenicity assessment. After biochemical test, PCR amplification, and serotype determination, it was confirmed that the isolated strain CZG-1 was serotype 15 R. anatipestifer. Typical spotted spleen symptoms were observed after CZG-1 infection. Furthermore, drug sensitivity assays showed the similar drug-resistant spectrum of R. anatipestifer serotype 15 to other serotypes; for example, all test strains were resistant to polymyxin, gentamicin, and neomycin. The CZG-1 strain has high pathogenicity, and its lethal dose of 50% (LD50) is 35.122 CFU/ml. Virulence gene determination showed that the CZG-1 strain had at least five virulence genes, bioF, TSS9-1, TSS9-2, PncA, and 0373Right. Above all, this study identified and proved that the pathogen of spotted spleen in ducks was R. anatipestifer serotype 15, which caused death of ducks without the typical symptoms of bacterial infection. The results of this study enriched the knowledge of symptom after R. anatipestifer infection, provided a reference to the identification of the pathogen of spotted spleen, and provided theoretical basis for prevention and control of spotted spleen.
Incidence and Predictors of Calf Morbidity and Mortality From Birth to 6-Months of Age in Dairy Farms of Northwestern Ethiopia
Authors: Alemu Yeshwas F. , Jemberu Wudu T. , Mekuriaw Zeleke , Abdi Reta Duguma
Published: May 2022

The Ethiopian government has initiatives for expanding the commercial and smallholder market-oriented urban and peri-urban dairy production systems to meet the demands for dairy products. However, there have been only limited on-farm studies on the health performance of commercial dairy breeds. The aim of this longitudinal study was to quantify the incidence and identify predictors of calf morbidity and mortality from birth to 6 months of age in urban and peri-urban dairy farms of Northwest Ethiopia. A total of 439 calves aged below 6 months from 174 dairy farms were included in the study. We collected data on 35 potential risk factors to determine their effect on calf morbidity and mortality in the area. Kaplan–Meier survival analysis was used to summarize survival probability. The Cox proportional hazard regression model with shared frailty to account for unmeasured herd-specific heterogeneity was also used to identify and quantify factors associated with time to morbidity and mortality. Among 439 calves enrolled for 6 months of follow-up period, a total of 141 morbidities and 54 mortality events were recorded. This gives an overall morbidity and mortality incidence rates of 64 per 100-calf 6-months at risk (risk rate of 47.3%) and 19 per 100-calf 6-months at risk (risk rate of 17.9%), respectively. Diarrhea was the most frequent calf health problem with a risk rate of 25.2%. It was the cause of death for 33.3% of all the 54 calf deaths. Next to diarrhea, pneumonia ranked second with risk rate of 8.6% and was responsible for death of 12.9% of all the 54 calf deaths. Among 35 potential risk factors, calf age, vigor status at birth, calf breed, colostrum ingestion, and herd size were significant (p < 0.05) predictors of calf morbidity and mortality. The Cox-shared frailty model revealed that the herd frailty component had no significant effect on hazard estimates of the covariates of all-cause morbidity and mortality. This implies that the dairy herds participated in the study were homogeneous in the distribution of unmeasured random effects. In conclusion, the magnitude of calf morbidity and mortality was higher and above economically tolerable level in this study. This could impede the success of Ethiopia's dairy development initiative in general, and the livelihood of smallholder dairy producers in particular. Therefore, educating farmers aimed at mitigating the identified risk factors can reduce calf morbidity and mortality in the study areas.
Food Media and Dietary Behavior in a Belgian Adult Sample: How Obtaining Information From Food Media Sources Associates With Dietary Behavior
Authors: Proesmans Viktor Lowie Juliaan , Vermeir Iris , de Backer Charlotte , Geuens Maggie
Published: May 2022

Objective: We aim to relate Flemish adults’ main food information sources (e.g., celebrity chefs, experts) with their dietary behavior.

Methods: A cross-sectional online survey among 1115 Flemish adults who regularly cook, measured the food information sources the respondents used to obtain recipes, their dietary intake and dietary restrictions. Ordinal and logistic regression were used to investigate the relation between food media, dietary intake and dietary restrictions.

Results: Celebrity chefs were mentioned most often (37%) as main food information source, followed by family and acquaintances (21%) and lifestyle gurus (12%). Using lifestyle gurus as a source of dietary information is associated with more dietary restrictions and a higher intake frequency of plant-based food groups, whereas using celebrity chefs or experts is associated with a different (but less unequivocal vegetarian or healthy) dietary intake.

Conclusion: Media icons like lifestyle gurus and celebrity chefs appear to be among people’s main sources of food information. There is a significant association between using them as a source of food information and dietary behavior. Further research on the influence of media on diet is required.
Organic Amendment Types Influence Soil Properties, the Soil Bacterial Microbiome, and Tomato Growth
Authors: Su Jia-Yang , Liu Cheng-Huan , Tampus Kimberly , Lin Ya-Chi , Huang Cheng-Hua
Published: May 2022

The overuse of chemical fertilizers deteriorates soil quality, and the application of organic amendments has been proposed as an alternative to mineral fertilizers. This study aimed to investigate the effects of organic amendment types on soil properties, the soil microbiome, and tomato growth. Tomato seedlings were grown in soils applied with ten fertilizer treatments, including a no fertilization control, a chemical fertilization control, and eight organic amendment treatments. Compared with the chemical fertilization treatment, the application of manure compost significantly increased the shoot dry weight of tomato plants. In addition, manure compost and tea seed meal remarkably increased soil organic matter (SOM) in comparison with the no fertilization and chemical fertilization treatments. Moreover, manure compost significantly increased soil-exchangeable K and Mg. The application of neem cake and manure compost significantly increased both bacterial diversity and richness. The relative abundance of Lysinibacillus was significantly positively related to the shoot and total dry weights of tomato plants, and its relative abundance was positively influenced by SOM and soil-exchangeable K. Overall, the manure compost used in this study can increase SOM, soil-exchangeable K and Mg, and the relative abundance of Lysinibacillus, consequently promoting tomato growth.
Description of Two New Cases of AQP1 Related Pulmonary Arterial Hypertension and Review of the Literature
Authors: Gallego-Zazo Natalia , Cruz-Utrilla Alejandro , del Cerro María Jesús , Ochoa Parra Nuria , Blanco Julián Nevado , Arias Pedro , Lapunzina Pablo , Escribano-Subias Pilar , Tenorio-Castaño Jair
Published: May 2022

Pulmonary arterial hypertension (PAH) is a severe clinical condition characterized by an increase in mean pulmonary artery pressure, which leads to a right ventricular hypertrophy and potentially heart failure and death. In the last several years, many genes have been associated with PAH, particularly in idiopathic and heritable forms but also in associated forms. Here we described the identification of two unrelated families in which the AQP1 variant was found from a cohort of 300 patients. The variants were identified by whole exome sequencing (WES). In the first family, the variant was detected in three affected members from a hereditary PAH, and in the second family the proband had PAH associated with scleroderma. In addition, we have reviewed all cases published in the literature thus far of patients with PAH and AQP1 variants. Functional studies have led to some contradictory conclusions, and the evidence of the relationship of AQP1 and PAH is still limited. However, we describe two further families with PAH and variants in AQP1, expanding both the number of cases and the clinically associated phenotype. We provide further evidence of the association of AQP1 and the development of hereditary and associated forms of PAH.
Major Histocompatibility Complex (MHC) Diversity of the Reintroduction Populations of Endangered Przewalski’s Horse
Authors: Tang Yongqing , Liu Gang , Zhao Shasha , Li Kai , Zhang Dong , Liu Shuqiang , Hu Defu
Published: May 2022

Major histocompatibility complex (MHC) genes are the most polymorphic in vertebrates and the high variability in many MHC genes is thought to play a crucial role in pathogen recognition. The MHC class II locus DQA polymorphism was analyzed in the endangered Przewalski’s horse, Equus przewalskii, a species that has been extinct in the wild and all the current living individuals descend from 12 founders. We used the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) to detect the polymorphism within the MHC DQA in 31 Przewalski’s horses from two reintroduced populations. Consequently, only seven alleles were identified, with only four presenting in each population. In comparison with other mammals, the Przewalski’s horse demonstrated less MHC variation. The nucleotide genetic distance of the seven ELA-DQA alleles was between 0.012 and 0.161. The Poisson corrected amino acid genetic distance of the founded alleles was 0.01–0.334. The allele and genotype frequencies of both reintroduced populations of Przewalski’s horse deviated from the Hardy–Weinberg equilibrium. Specific MHC DQA alleles may have been lost during the extreme bottleneck event that this species underwent throughout history. We suggest the necessity to detect the genetic background of individuals prior to performing the reintroduction project.
Construction of Ecological Security Patterns Based on Circuit Theory under the Resistance Distance Principle
Authors: Chen Jinzhao , Mei Zhixiong , Wang Bin , Wei Junchao
Published: May 2022

Against the background of China’s advocating ecological civilisation construction, an urgent task and a major challenge are to identify key places for ecological protection and restoration and then propose optimisation strategies for future land use, especially in the Pearl River Delta (PRD), one of the regions in China that has the highest urbanisation level. In this study, we find the key places by constructing ecological security patterns and proposing optimisation strategies for future land use by analysing land-use status. We also propose a source identification method based on the resistance distance principle. Results show that forty-six sources were mainly distributed in the mountainous areas surrounding PRD but were less distributed along both sides of the Pearl River estuary. The difference in the spatial distribution of sources is remarkable. Eighty-four corridors generally had spider-like shapes. In the central plain of PRD, corridors were relatively long and narrow. Ninety pinch points were concentrated on existing rivers. Three barriers were located in the corridors between adjacent sources. Two artificial corridors were proposed to be established, which can improve the ecological network connectivity. The method for extracting sources based on the resistance distance principle is proven to be advantageous for improving the integrity of source extraction results and making ecological security patterns more reasonable.
Potential Energy as an Alternative for Assessing Lower Limb Peak Power in Children: A Bayesian Hierarchical Analysis
Authors: Fernandez-Santos Jorge R. , Gutierrez-Manzanedo Jose V. , Arroyo-Garcia Pelayo , Izquierdo-Jurado Jose , Gonzalez-Montesinos Jose L.
Published: May 2022

The aim of this study was to analyze the use of potential energy (PE) as an alternative method to assess peak power of the lower limbs (PP) in children. 815 Spanish children (416 girls; 6–11 years old; Body Mass Index groups (n): underweight = 40, normal weight = 431, overweight = 216, obese = 128) were involved in this study. All participants performed a Countermovement Jump (CMJ) test. PP was calculated using Duncan (PPDUNCAN), Gomez-Bruton (PPGOMEZ) and PECMJ formulas. A model with PECMJ as the predictor variable showed a higher predictive accuracy with PPDUNCAN and PPGOMEZ than CMJ height (R2 = 0.99 and 0.97, respectively; ELPDdiff = 1037.0 and 646.7, respectively). Moreover, PECMJ showed a higher linear association with PPDUNCAN and PPGOMEZ across BMI groups than CMJ height (βPECMJ range from 0.67 to 0.77 predicting PPDUNCAN; and from 0.90 to 1.13 predicting PPGOMEZ). Our results provide further support for proposing PECMJ as an index to measure PP of the lower limbs, taking into account the children’s weight and not only the height of the jump. Therefore, we suggest the use of PECMJ in physical education classes as a valid method for estimating PP among children when laboratory methods are not feasible.
Quantitative Analysis of the Transcriptome of Two Commonly Used Human Monocytic Cell Lines—THP-1 and Mono Mac 6—Reveals Their Arrest during Early Monocyte/Neutrophil Differentiation
Authors: Akula Srinivas , Lara Sandra , Olsson Anna-Karin , Hellman Lars
Published: May 2022

Cell lines of monocyte/macrophage origin are often used as model systems to study monocyte/macrophage biology. A relevant question is how similar these cell lines are to their in vivo counterparts? To address this issue, we performed a detailed analysis of the transcriptome of two commonly used human monocyte/macrophage cell lines, Mono Mac 6 and THP-1. Both of these cell lines originate from leukemic cells with myelo-monocytic characteristics. We found that both Mono Mac 6 and THP-1 represent cells of very immature origin. Their transcriptomes show more similarities to immature neutrophils than cells of the monocyte/macrophage lineage. They express significant levels of N-elastase, proteinase 3, cathepsin G, and azurocidin but very low levels of CD14, ficolin, and complement factor P. All major MHC class II genes are also expressed at low levels. They show high levels of lysozyme and low levels of one of the immunoglobulin Fc receptors, FCGRIIA, which is characteristic of both neutrophils and monocytes. THP-1, but not Mono Mac 6, also expresses the high-affinity receptor for IgG, FCGRIA. Both cell lines lack the expression of the connective tissue components fibronectin, proteoglycan 4, and syndecan 3, which are characteristics of tissue macrophages but are absent in blood monocytes, indicating that they originate from bone marrow precursors and not yolk sac-derived hematopoietic cells. Both of these cell lines seem, therefore, to represent cells arrested during early myelo-monocytic development, at a branch point between neutrophil and monocyte differentiation. Their very immature phenotype indicates that great care should be taken when using these cell lines as models for normal monocyte/macrophage biology.
The Rice Malectin Regulates Plant Cell Death and Disease Resistance by Participating in Glycoprotein Quality Control
Authors: Feng Huijing , Qiu Tiancheng , Yin Changfa , Zhao Xiaosheng , Xu Guangyuan , Qi Linlu , Zhang Yan , Peng Youliang , Zhao Wensheng
Published: May 2022

In animals, malectin is well known to play an essential role in endoplasmic reticulum quality control (ERQC) by interacting with ribophorin I, one unit of the oligosaccharyltransferase (OST) complex. However, the functions of malectin in plants remain largely unknown. Here, we demonstrate the rice OsMLD1 is an ER- and Golgi-associated malectin protein and physically interacts with rice homolog of ribophorin I (OsRpn1), and its disruption leads to spontaneous lesion mimic lesions, enhanced disease resistance, and prolonged ER stress. In addition, there are many more N-glycosites and N-glycoproteins identified from the mld1 mutant than wildtype. Furthermore, OsSERK1 and OsSERK2, which have more N-glycosites in mld1, were demonstrated to interact with OsMLD1. OsMLD1 can suppress OsSERK1- or OsSERK2-induced cell death. Thus, OsMLD1 may play a similar role to its mammalian homologs in glycoprotein quality control, thereby regulating cell death and immunity of rice, which uncovers the function of malectin in plants.
Neurogenesis Is Increased in Human Neural Stem Cells by Aβ40 Peptide
Authors: Bernabeu-Zornoza Adela , Coronel Raquel , Palmer Charlotte , Martín Alberto , López-Alonso Victoria , Liste Isabel
Published: May 2022

Amyloid-β 40 peptides [Aβ1-40 (Aβ40)] are present within amyloid plaques in the brains of patients with Alzheimer’s disease (AD). Even though Aβ peptides are considered neurotoxic, they can mediate many biological processes, both in adult brains and throughout brain development. However, the physiological function of these Aβ peptides remains poorly understood, and the existing data are sometimes controversial. Here, we analyze and compare the effects of monomeric Aβ40 on the biology of differentiating human neural stem cells (human NSCs). For that purpose, we used a model of human NSCs called hNS1. Our data demonstrated that Aβ40 at high concentrations provokes apoptotic cellular death and the damage of DNA in human NSCs while also increasing the proliferation and favors neurogenesis by raising the percentage of proliferating neuronal precursors. These effects can be mediated, at least in part, by β-catenin. These results provide evidence of how Aβ modulate/regulate human NSC proliferation and differentiation, suggesting Aβ40 may be a pro-neurogenic factor. Our data could contribute to a better understanding of the molecular mechanisms involved in AD pathology and to the development of human NSC-based therapies for AD treatment, since these results could then be used in diagnosing the disease at early stages and be applied to the development of new treatment options.
Modeling the Potential Global Distribution of Honeybee Pest, Galleria mellonella under Changing Climate
Authors: Hosni Eslam M. , Al-Khalaf Areej A. , Nasser Mohamed G. , Abou-Shaara Hossam F. , Radwan Marwa H.
Published: May 2022

Beekeeping is essential for the global food supply, yet honeybee health and hive numbers are increasingly threatened by habitat alteration, climate change, agrochemical overuse, pathogens, diseases, and insect pests. However, pests and diseases that have unknown spatial distribution and influences are blamed for diminishing honeybee colonies over the world. The greater wax moth (GWM), Galleria mellonella, is a pervasive pest of the honeybee, Apis mellifera. It has an international distribution that causes severe loss to the beekeeping industry. The GWM larvae burrow into the edge of unsealed cells that have pollen, bee brood, and honey through to the midrib of the wax comb. Burrowing larvae leave behind masses of webs that cause honey to leak out and entangle emerging bees, resulting in death by starvation, a phenomenon called galleriasis. In this study, the maximum entropy algorithm implemented in (Maxent) model was used to predict the global spatial distribution of GWM throughout the world. Two representative concentration pathways (RCPs) 2.6 and 8.5 of three global climate models (GCMs), were used to forecast the global distribution of GWM in 2050 and 2070. The Maxent models for GWM provided a high value of the Area Under Curve equal to 0.8 ± 0.001, which was a satisfactory result. Furthermore, True Skilled Statistics assured the perfection of the resultant models with a value equal to 0.7. These values indicated a significant correlation between the models and the ecology of the pest species. The models also showed a very high habitat suitability for the GWM in hot-spot honey exporting and importing countries. Furthermore, we extrapolated the economic impact of such pests in both feral and wild honeybee populations and consequently the global market of the honeybee industry.
Investigation of Negative Bias Temperature Instability Effect in Nano PDSOI PMOSFET
Authors: Yang Yafang , Liu Hongxia , Yang Kun , Gao Zihou , Liu Zixu
Published: May 2022

The Negative Bias Temperature Instability (NBTI) effect of partially depleted silicon-on-insulator (PDSOI) PMOSFET based on 130 nm is investigated. First, the effect of NBTI on the IV characteristics and parameter degradation of T-Gate PDSOI PMOSFET was investigated by accelerated stress tests. The results show that NBTI leads to a threshold voltage negative shift, saturate drain current reduction and transconductance degradation of the PMOSFET. Next, the relationship between the threshold voltage shift and stress time, gate bias and temperature, and the channel length is investigated, and the NBTI lifetime prediction model is established. The results show that the NBTI lifetime of a 130 nm T-Gate PDSOI PMOSFET is approximately 18.7 years under the stress of VG = −1.2 V and T = 125 °C. Finally, the effect of the floating-body effect on NBTI of PDSOI PMOSFET is investigated. It is found that the NBTI degradation of T-Gate SOI devices is greater than that of the floating-body SOI devices, which indicates that the floating-body effect suppresses the NBTI degradation of SOI devices.