bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparable outcomes from long and short read random sequencing of total RNA for detection of pathogens in chicken respiratory samples
Authors: Butt Salman L. , Kariithi Henry M. , Volkening Jeremy D. , Taylor Tonya L. , Leyson Christina , Pantin-Jackwood Mary , Suarez David L. , Stanton James B. , Afonso Claudio L.
Published: December 2022

Co-infections of avian species with different RNA viruses and pathogenic bacteria are often misdiagnosed or incompletely characterized using targeted diagnostic methods, which could affect the accurate management of clinical disease. A non-targeted sequencing approach with rapid and precise characterization of pathogens should help respiratory disease management by providing a comprehensive view of the causes of disease. Long-read portable sequencers have significant potential advantages over established short-read sequencers due to portability, speed, and lower cost. The applicability of short reads random sequencing for direct detection of pathogens in clinical poultry samples has been previously demonstrated. Here we demonstrate the feasibility of long read random sequencing approaches to identify disease agents in clinical samples. Experimental oropharyngeal swab samples (n = 12) from chickens infected with infectious bronchitis virus (IBV), avian influenza virus (AIV) and Mycoplasma synoviae (MS) and field-collected clinical oropharyngeal swab samples (n = 11) from Kenyan live bird markets previously testing positive for Newcastle disease virus (NDV) were randomly sequenced on the MinION platform and results validated by comparing to real time PCR and short read random sequencing in the Illumina MiSeq platform. In the swabs from experimental infections, each of three agents in every RT-qPCR-positive sample (Ct range 19–34) was detectable within 1 h on the MinION platform, except for AIV one agent in one sample (Ct = 36.21). Nine of 12 IBV-positive samples were assigned genotypes within 1 h, as were five of 11 AIV-positive samples. MinION relative abundances of the test agent (AIV, IBV and MS) were highly correlated with RT-qPCR Ct values (R range−0.82 to−0.98). In field-collected clinical swab samples, NDV (Ct range 12–37) was detected in all eleven samples within 1 h of MinION sequencing, with 10 of 11 samples accurately genotyped within 1 h. All NDV-positive field samples were found to be co-infected with one or more additional respiratory agents. These results demonstrate that MinION sequencing can provide rapid, and sensitive non-targeted detection and genetic characterization of co-existing respiratory pathogens in clinical samples with similar performance to the Illumina MiSeq.
Turismo sostenible: el diseño como catalizador de espacios de interacción cultural en la laguna de Colta en los Andes ecuatorianos
Authors: Núñez-Torres Sandra Hipatia , Fuentes-Pérez Eliska , Balseca-Clavijo Claudia Rafaela , Álvarez-Lizano Iván Patricio
Published: December 2022

El turismo sostenible está orientado a conservar el capital natural y cultural de las comunidades, y, al mismo tiempo, fraguar ingresos a la localidad. Para generar turismo sostenible se precisa comprender las necesidades del grupo social involucrado. A este respecto la función social del diseño conlleva a satisfacer necesidades humanas individuales y colectivas actuando como mediador en el desarrollo económico social de la comunidad. Pensar en espacios turísticos como la laguna de Colta o Kulta Kucha, históricamente desaprovechado en la difusión del turismo, resulta de interés, pues, constituye un ícono ancestral de las poblaciones indígenas de la sierra ecuatoriana. Interés que crece al considerar que en los últimos años factores como el restablecimiento de su acceso férreo y la restauración de su patrimonio arquitectónico colonial le han catapultado como potencial destino turístico. Desde esta perspectiva, la propuesta procura considerar al diseño desde el enfoque social e interdisciplinario, puesto que, es una disciplina socialmente construida, y que, por ende, apela a los recursos culturales y naturales para potencializar la generación de turismo sostenible en dicha laguna, considerando un enfoque cualitativo ya que se orienta hacia el sentido de los fenómenos, indagándolos desde el punto de vista de los actores en su hábitat natural y en relación con el contexto actual e histórico. De esta manera, en principio se planifica la identificación e interpretación de necesidades de desarrollo comunitario sostenible, la identificación y análisis de recursos culturales y naturales presentes en el contexto para determinar su perspectiva de uso, valores expresivos y simbólicos y por último establecer lineamientos en la dimensión proyectual del diseño para la planificación de modelos de turismo sostenible que viabilicen el desarrollo económico, social y productivo de la comunidad. En este sentido, a través de la apropiación de recursos que materializan el entorno se plantea la posibilidad de pensar en modelos estructurales funcionales de interacción turística y comunitaria.
Magnetic, Thermal, and Transport Properties of Co2Ti1.5Sn0.5 Heusler Alloy
Authors: Athul Sunitha Raveendran , Arun Kumar , Swathi Saktivel , Remya Uralath Dhanavardhanan , Dzubinska Andrea , Reiffers Marian , Nagalakshmi Ramamoorti
Published: December 2022

In this work, the structural, magnetic, thermal, and transport properties of the arc-melted polycrystalline Heusler alloy Co2Ti1.5Sn0.5 are investigated. The alloy crystallizes in an L21 structure with a space group of Fm-3m. The magnetic properties of the alloy depict its antiferromagnetic nature and the alloy exhibits magnetic ordering around Neel Temperature TN = 8.5 K. The effective magnetic moment value obtained from the Curie –Weiss law suggests that the cobalt atom in the alloy is in the low-spin state. From the heat capacity studies, the Sommerfeld coefficient and Debye temperature were determined. In addition, electrical resistivity shows a linear response with increasing temperature, indicating the metallic nature of the alloy.
An Improved Hybrid Control Scheme of a Switched Reluctance Motor for Torque Ripple Reduction
Authors: He Xiaofeng , Yao Yao
Published: December 2022

In this paper, we presents an improved hybrid control scheme based on model predictive torque control using linear active disturbance rejection control (LADRC) for the torque drive system of a 12/14 bearingless switched reluctance motor. The proposed approach can considerably reduce the torque ripple and enhance the anti-disturbance ability. First, a modified piecewise torque sharing function (TSF) is applied to reduce the torque ripple in the commutation interval. Second, model predictive control is introduced to further reduce the torque ripple caused by the hysteresis control. The selection of the optimal weighting factor is avoided, as the current value corresponding to different positions of each phase is determined by the TSF; thus, the cost function can be simplified. Then, a speed controller is designed by an enhanced LADRC for improved tracking performance and anti-disturbance ability, whereby a proportional gain of observation error and a time-varying function are investigated in extended-state observer. Finally, experiments are carried out to verify the effectiveness of the hybrid control scheme by comparison with cosine TSF function and conventional LADRC.
Image Fundus Classification System for Diabetic Retinopathy Stage Detection Using Hybrid CNN-DELM
Authors: Novitasari Dian Candra Rini , Fatmawati Fatmawati , Hendradi Rimuljo , Rohayani Hetty , Nariswari Rinda , Arnita Arnita , Hadi Moch Irfan , Saputra Rizal Amegia , Primadewi Ardhin
Published: December 2022

Diabetic retinopathy is the leading cause of blindness suffered by working-age adults. The increase in the population diagnosed with DR can be prevented by screening and early treatment of eye damage. This screening process can be conducted by utilizing deep learning techniques. In this study, the detection of DR severity was carried out using the hybrid CNN-DELM method (CDELM). The CNN architectures used were ResNet-18, ResNet-50, ResNet-101, GoogleNet, and DenseNet. The learning outcome features were further classified using the DELM algorithm. The comparison of CNN architecture aimed to find the best CNN architecture for fundus image features extraction. This research also compared the effect of using the kernel function on the performance of DELM in fundus image classification. All experiments using CDELM showed maximum results, with an accuracy of 100% in the DRIVE data and the two-class MESSIDOR data. Meanwhile, the best results obtained in the MESSIDOR 4 class data reached 98.20%. The advantage of the DELM method compared to the conventional CNN method is that the training time duration is much shorter. CNN takes an average of 30 min for training, while the CDELM method takes only an average of 2.5 min. Based on the value of accuracy and duration of training time, the CDELM method had better performance than the conventional CNN method.
The Relationship between Structural Features of Lignocellulosic Materials and Ethanol Production Yield
Authors: Bay Mohammad Saber , Eslami Fatemeh , Karimi Keikhosro
Published: December 2022

Lignocellulosic materials are a mixture of natural polymers which can be considered a great alternative source of chemical products and energy. Hence, pinewood, poplar wood, and rice straw, as representatives of different types of lignocelluloses, were subjected to several pretreatment types in order to increase ethanol production yield. All pretreatments increased enzymatic hydrolysis and ethanol yield, specifically pretreatment with phosphoric acid. This pretreatment increased ethanol yields by 304.6% and 273.61% for poplar wood and pinewood, respectively, compared to untreated substrates. In addition, a number of analyses, including a BET test, buffering capacity, crystallinity, accessible surface area, and composition measurement, were conducted on the pretreated substrates to investigate their structural modifications in detail. Accessible surface area, as one of the most important parameters for performance of enzymes and microorganisms in the fermentation process, was examined by the water retention value test. The results of this method (using centrifuge) showed that the maximum accessible surface area was related to the pretreated samples with phosphoric acid so that it increased WRV to 132.19%, 149.41%, and 68.44% for poplar wood, pinewood, and rice straw, respectively, as compared to untreated substrates. On the whole, pretreatments restructured and opened up the tangled structure of lignocelluloses, resulting in a considerable increase in ethanol yields. Moreover, in this study, for the first time, a new correlation was presented for each substrate which indicates the relationship between ethanol yield and structural features of the lignocellulosic substrate.
Methoxyflurane for Relief of Procedural Pain in Burn Patients: A Prospective Single-Centre Evaluation Study
Authors: Creutzburg Andreas , Vestergaard Martin R. , Pape Pernille , Hjelmdal Caroline , Rangatchew Filip , Holmgaard Rikke , Rasmussen Lars S.
Published: December 2022

Background: Procedural pain in burn patients continues to be a major problem. Frequently used analgesics, such as opioids, may have various side effects, including respiratory depression, nausea, and vomiting. Inhaled methoxyflurane has been used in the pre-hospital setting for trauma-related pain. This pilot study aimed to investigate the feasibility of using methoxyflurane for pain relief during dressings changes for burns in the hospital setting. Methods: In this investigator-initiated pilot study, we included burn patients undergoing dressing changes in the burn ward. The primary outcome was the maximal pain level experienced by the patient during the procedure on a verbal rating scale of 0 to 100. Furthermore, patient satisfaction and the nurse’s assessment of the patient’s pain were reported. We also reported the presence of nausea, vomiting, coughing, and headache, along with changes in the pulse rate, oxygen saturation, and arterial blood pressure. Results: We included 12 patients in the period of June 2021 to July 2022. The median patient-reported maximal procedural pain was 60 (interquartile range (IQR), 37–80), which corresponded well with the nurse’s rating of a median of 57 (IQR 28–67). The patients were satisfied with methoxyflurane as an analgesic, with a median score of 96 (IQR 96–100). One patient reported coughing after the procedure, and another patient experienced nausea one week after the procedure. No clinically important haemodynamic changes during administration were detected. Conclusions: Methoxyflurane was found to be feasible for pain relief in burn patients undergoing dressing changes in the burn ward.
Spatiotemporal Analysis of Hydrogen Requirement to Minimize Seasonal Variability in Future Solar and Wind Energy in South Korea
Authors: Oh Myeongchan , Kim Boyoung , Yun Changyeol , Kim Chang Ki , Kim Jin-Young , Hwang Su-Jin , Kang Yong-Heack , Kim Hyun-Goo
Published: December 2022

Renewable energy supply is essential for carbon neutrality; however, technologies aiming to optimally utilize renewable energy sources remain insufficient. Seasonal variability in renewable energy is a key issue, which many studies have attempted to overcome through operating systems and energy storage. Currently, hydrogen is the only technology that can solve this seasonal storage problem. In this study, the amount of hydrogen required to circumvent the seasonal variability in renewable energy supply in Korea was quantified. Spatiotemporal analysis was conducted using renewable energy resource maps and power loads. It was predicted that 50% of the total power demand in the future will be met using solar and wind power, and a scenario was established based on the solar-to-wind ratio. It was found that the required hydrogen production differed by approximately four-times, depending on the scenarios, highlighting the importance of supplying renewable energy at an appropriate ratio. Spatially, wind power was observed to be unsuitable for the physical transport of hydrogen because it has a high potential at mountain peaks and islands. The results of this study are expected to aid future hydrogen research and solve renewable energy variability problems.
Experimental Investigations on Detonation Initiation Characteristics of a Liquid-Fueled Pulse Detonation Combustor at Different Inlet Air Temperatures
Authors: Tan Wenhao , Zheng Longxi , Lu Jie , Wang Lingyi , Zhou Daoen
Published: December 2022

The detonation initiation characteristics of a single tube liquid-fueled pulse detonation combustor (PDC) is investigated at different inlet air temperatures in this paper. The inner diameter of the PDC is 62 mm. Gasoline and air are used as fuel and oxidant, respectively. The inlet air temperature is 288–523 K and the operating frequency of the PDC is 10~30 Hz. The experimental results show that the deflagration to detonation transition (DDT) distance, detonation initiation time, DDT time and jet ignition time decrease with the increasing operating frequency at the same inlet temperature. When the inlet temperature is 288 K, the DDT distance is shortened from 860.5 mm to 787.7 mm as the operating frequency increases from 10 Hz to 30 Hz. The detonation initiation time, the jet ignition time and the DDT time are reduced from 10.01 ms, 7.66 ms and 2.35 ms to 6.55 ms, 4.99 ms and 1.56 ms, respectively. When the inlet air temperature increases, the atomization and evaporation of the gasoline is improved, which also leads to the decrease in the DDT distance, the detonation initiation time, the jet ignition time and the DDT time. For example, when the inlet air temperature increases from 288 K to 523 K at the frequency of 10 Hz, the DDT distance is shortened from 860.5 mm to 747.2 mm and the detonation initiation time, the jet ignition time and the DDT time is reduced to 5.867 ms, 2.51 ms and 1.11 ms, respectively. Additionally, the self-ignition caused by high inner wall temperature occurs when PDC is operating at high frequency under high inlet air temperature.
An Investigation of Employees’ Intention to Comply with Information Security System—A Mixed Approach Based on Regression Analysis and fsQCA
Authors: Li Wenqin , Liu Rongmin , Sun Linhui , Guo Zigu , Gao Jie
Published: December 2022

Employee security compliance behavior has become an important safeguard to protect the security of corporate information assets. Focusing on human factors, this paper discusses how to regulate and guide employees’ compliance with information security systems through effective methods. Based on protection motivation theory (PMT), a model of employees’ intention to comply with the information security system was constructed. A questionnaire survey was adopted to obtain 224 valid data points, and SPSS 26.0 was applied to verify the hypotheses underlying the research model. Then, based on the results of a regression analysis, fuzzy set qualitative comparative analysis (fsQCA) was used to explore the conditional configurations that affect employees’ intention to comply with the information security system from a holistic perspective. The empirical results demonstrated that perceived severity, perceived vulnerability, response efficacy, and self-efficacy all positively influenced the employees’ intention to comply with the information security system; while rewards and response costs had a negative effect. Threat appraisal had a greater effect on employees’ intention to comply with the information security system compared to response appraisal. The fsQCA results showed that individual antecedent conditions are not necessary to influence employees’ intention to comply with an information security system. Seven pathways exist that influence an employees’ intention to comply with an information security system, with reward, self-efficacy, and response cost being the core conditions having the highest probability of occurring in each configuration of pathways, and with perceived severity and self-efficacy appearing in the core conditions of configurations with an original coverage greater than 40%. Theoretically, this study discusses the influence of the elements of PMT on employees’ intention to comply with an information security system, reveals the mechanism of influence of the combination of the influencing factors on the outcome variables, and identifies the core factors and auxiliary factors in the condition configurations, providing a new broader perspective for the study of information security compliance behavior and providing some theoretical support for strengthening enterprise security management. Practically, targeted suggestions are proposed based on the research results, to increase the intention of enterprise employees to comply with information security systems, thereby improving the effectiveness of enterprise information security management and the degree of information security in enterprises.
High Prevalence of Hyperuricemia and Associated Factors among Zhuang Adults: A Cross-Sectional Study Based on the Ethnic Minority Population Cohort in the Southwestern China
Authors: Zhong Lixian , Liu Shun , Qiu Xiaoqiang , Zeng Xiaoyun , Su Li , Huang Dongping , Guo Xiaojing , Liang Jun , Yang Yu , Tang Xiaofen , Xie Yihong
Published: December 2022

The highest prevalence of hyperuricemia was found in Zhuang minority adults in two national surveys in China, with only 1% Zhuang study subjects. However, the prevalence of hyperuricemia and the associated factors in Zhuang adults have not been well-addressed. A cross-sectional study was conducted to explore the prevalence of hyperuricemia and the common comorbidities, and the associated factors in Zhuang adults based on the Guangxi Ethnic Minority Population Cohort. Among 11,175 Zhuang adults aged 35–74 years, the age- and sex-standardized prevalence rate was 18.1% for hyperuricemia and 1.1% for gout. The standardized prevalence rate and awareness rate were 31.6% and 32.3%, respectively, for hypertension, and were 5.1% and 48.2%, respectively, for diabetes. High education level, history of coronary heart disease (CHD), hypertension, being a current drinker, high body mass index (BMI), central obesity, hyper-triglyceride (hyper-TG), hyper-total cholesterol (hyper-TC), hypo-high density lipoprotein cholesterol (hypo-HDL-C), and abnormal aspartate aminotransferase (AST) were risk factors, while smoking and diabetes were protective factors of hyperuricemia in males. Older age, being single/divorced, having a high education level, hypertension, drinking tea, high BMI, central obesity, hyper-TG, hyper-TC, hypo-HDL-C, and abnormal alanine aminotransferase (ALT) were risk factors in females. The high prevalence of hyperuricemia but low prevalence of gout and common comorbidities in Zhuang adults may be due to a lag effect of lifestyle changes. Health education and health management should be strengthened to prevent the progression of comorbidities, considering the lag effect and low awareness rate.
Exploring the Nonlinear and Threshold Effects of Travel Distance on the Travel Mode Choice across Different Groups: An Empirical Study of Guiyang, China
Authors: He Mingwei , Li Jianbo , Shi Zhuangbin , Liu Yang , Shuai Chunyan , Liu Jie
Published: December 2022

Examining how travel distance is associated with travel mode choice is essential for understanding traveler travel patterns and the potential mechanisms of behavioral changes. Although existing studies have explored the effect of travel distance on travel mode choice, most overlook their non-linear relationship and the heterogeneity between groups. In this study, the correlation between travel distance and travel mode choice is explored by applying the random forest model based on resident travel survey data in Guiyang, China. The results show that travel distance is far more important than other determinants for understanding the mechanism of travel mode choice. Travel distance contributes to 42.28% of explanation power for predicting travel mode choice and even 63.24% for walking. Significant nonlinear associations and threshold effects are found between travel distance and travel mode choice, and such nonlinear associations vary significantly across different socioeconomic groups. Policymakers are recommended to understand the group heterogeneity of travel mode choice behavior and to make targeted interventions for different groups with different travel distances. These results can provide beneficial guidance for optimizing the spatial layout of transportation infrastructure and improving the operational efficiency of low-carbon transportation systems.
Identifying Evacuation Needs and Resources Based on Volunteered Geographic Information: A Case of the Rainstorm in July 2021, Zhengzhou, China
Authors: Gao Jingyi , Murao Osamu , Pei Xuanda , Dong Yitong
Published: December 2022

Recently, global climate change has led to a high incidence of extreme weather and natural disasters. How to reduce its impact has become an important topic. However, the studies that both consider the disaster’s real-time geographic information and environmental factors in severe rainstorms are still not enough. Volunteered geographic information (VGI) data that was generated during disasters offered possibilities for improving the emergency management abilities of decision-makers and the disaster self-rescue abilities of citizens. Through the case study of the extreme rainstorm disaster in Zhengzhou, China, in July 2021, this paper used machine learning to study VGI issued by residents. The vulnerable people and their demands were identified based on the SOS messages. The importance of various indicators was analyzed by combining open data from socio-economic and built-up environment elements. Potential safe areas with shelter resources in five administrative districts in the disaster-prone central area of Zhengzhou were identified based on these data. This study found that VGI can be a reliable data source for future disaster research. The characteristics of rainstorm hazards were concluded from the perspective of affected people and environmental indicators. The policy recommendations for disaster prevention in the context of public participation were also proposed.
Lurasidone use in Cannabis-Induced Psychosis: A Novel Therapeutic Strategy and Clinical Considerations in Four Cases Report
Authors: Ricci Valerio , Martinotti Giovanni , De Berardis Domenico , Maina Giuseppe
Published: December 2022

Background: Lurasidone is an atypical antipsychotic approved for the acute and maintenance treatment of schizophrenia. Recently, lurasidone was also extended FDA approval for adults with major depressive episodes associated with bipolar I disorder (bipolar depression), as either a monotherapy or as adjunctive therapy with lithium or valproate. The use of low doses of atypical antipsychotics is an essential component of early intervention in psychosis, but little has yet been studied on first episode cannabis-induced psychosis. For its particular performance and tolerability, lurasidone is becoming an important option for the treatment of first-episode psychosis in youth. Case presentation four patients experiencing first cannabis-induced psychotic episode were treated with lurasidone. In all patients, there was an improvement in the clinical picture of psychosis. The recovery was positive, not only with the remission of positive and negative symptoms, but also regarding disruptive behaviour, with the return of functioning. All the patients were treated with lurasidone, with a target dose of 74–128 mg/day. No significant side effects were reported. Conclusion: There are non-controlled studies for the use of lurasidone in first episode psychosis cannabis induced. These findings suggest that lurasidone is an atypical antipsychotic beneficial in this clinical picture. Treatment with medium-high doses of lurasidone could be effective and tolerable in this phase of the disorder. Randomized control trials with longer follow-up are recommended to confirm these positive results.
Hypocalcemia Is a Common Risk Factor for Osteoporosis in Taiwanese Patients with Cushing’s Syndrome
Authors: Chen Yung-Nien , Tsai Jia-Ruei , Chen Jung-Fu , Shen Feng-Chih
Published: December 2022

Background: Osteoporosis is a cardinal manifestation of Cushing’s syndrome. There is a lack of relevant research on risk factors for osteoporosis among patients with Cushing’s syndrome (CS) in Taiwan. Thus, this study was designed to explore the possible risk factors of osteoporosis. Methods: We gathered patients with a diagnosis of CS between 2001 and 2017 in the Chang Gung Research Database (CGRD). We extracted data including diagnoses and biochemistry from hospital records. The diagnosis of CS was based on ICD-9-CM codes (255.0). Osteoporosis was defined by a T value equal to or less than −2.5 in BMD examination and hypocalcemia was defined as serum calcium concentrations < 8.0 mg/dL. Results: A total of 356 patients with CS who made regular visits to the outpatient department were enrolled in this study. The mean age was 68.6 years, and 74.9% of the patients were female. Of them, 207 patients (58.1%) were diagnosed with osteoporosis. Multivariable logistic regression models indicated that serum calcium level was negatively associated with osteoporosis (OR 0.70, CI 0.54–0.91, p < 0.001) after adjustment for age, sex, and other confounding risk factors. In addition, hypocalcemia was associated with heart failure (HF) (OR 2.14, CI 1.02–4.47, p < 0.05), stroke (OR 2.58, CI 1.21–5.46, p < 0.05) and osteoporosis (OR 3.04, CI 1.24–7.41, p < 0.05) in multivariate analysis. Conclusions: Our study found that lower serum calcium levels were common among patients with CS and osteoporosis. Furthermore, CS patients with HF or stroke had high proportion of hypocalcemia. Therefore, these patients must pay more attention to adequate calcium supplementation and undergo the appropriate osteoporosis drug treatment to reduce the risk of subsequent fracture and disability.
The Effect of Chronotype on Risk-Taking Behavior: The Chain Mediation Role of Self-Control and Emotional Stability
Authors: Zhang Qinfei , Wang Xu’an , Miao Lvqing , He Lichun , Wang Huarong
Published: December 2022

Background: Although previous studies indicate that chronotype might be associated with risk-taking behavior, the specific mechanism has not been thoroughly discussed. This study aimed to fill this gap by exploring the mediating role of self-control and the chain mediating role of self-control and emotional stability between chronotype and risk-taking behavior. Methods: A total of 547 Chinese college students between 18 and 24 years old were selected to complete the Morningness–Eveningness Questionnaire (MEQ), Self-Control Scale (SCS), Eysenck’s Personality Questionnaire-neuroticism (EPQ-N), and Adolescent Risk-Taking Questionnaire: Risk Behavior Scale (ARQ-RB) to assess chronotype, risk-taking behavior, self-control, and emotional stability, respectively. Hayes’ PROCESS macro for SPSS was used to test the relationships among these variables. Results: Our result showed significant positive correlations among chronotype, self-control, emotional stability, and significant negative correlations between self-control, emotional stability, and risk-taking behavior. We also found that chronotype had a significant predictive effect on risk-taking behavior in the chain mediation model. Specifically, chronotype affected risk-taking behavior through two pathways: the separate mediating role of self-control and the serial mediation pathway of self-control → emotional stability. Conclusions: Our study provides direct evidence that chronotype is associated with risk-taking behavior. The results showed that the predictive function of chronotype was mediated by self-control and emotional stability. This study provides a new perspective on preventing and reducing risk-taking behavior.
A Study on the Psychological Attributes of Survivors Who Experienced Downsizing in China
Authors: Lee Byung Hee , Cai Li , Liu Jing , Chang Yu Jin
Published: December 2022

This study aims to examine how perceived justice affects downsizing survivors’ attitudes, from the psychological contract perspective. By using data collected through surveys from employees of the Industrial and Commercial Bank of China (ICBC) who have recently survived layoffs, we examine the relationships between perceived justice, the survivors’ psychological status, and their attitudes after downsizing. The hypothesis was verified through path analysis using SPSS 26.0 and Amos 23.0. Our findings are as follows. Perceived justice has a negative effect on psychological contract violation and a positive effect on trust. Psychological contract violation influences affective commitment negatively and influences turnover intention positively. Trust is positively related to affective commitment and negatively related to turnover intention. We hope that this study will be a useful piece of data that can provide guidelines for inducing positive behavior of members in downsized organizations.
Gender Differences in the Indirect Effect of Psychosocial Work Environment in the Association of Precarious Employment and Chronic Stress: A Cross-Sectional Mediation Analysis
Authors: Méndez-Rivero Fabrizio , Pozo Óscar J. , Julià Mireia
Published: December 2022

Gender differences in the association between precarious employment and chronic stress have been found but the mechanisms underlying this relationship have not been explored. The main objective of this study was to evaluate the mediating effects of psychosocial risk factors at work (i.e., demands, control, and support) and work–life conflicts in the relationship between precarious employment and chronic stress as measured through the production of steroid hormones (both adrenal and gonadal) for men and women separately. Cross-sectional data were derived from a sample of workers from Barcelona (n = 125–255 men; 130 women). A set of 23 markers were determined from hair samples to evaluate the production of both adrenal and gonadal steroids. Decomposition analyses were applied to estimate the indirect effects of psychosocial risk factors and work–life conflict using linear regression models. Gender differences in the association between precarious employment and steroids production were confirmed. Psychosocial risk factors and work–life conflicts had indirect effects only among women (βCortisol = 0.18; 95% CI: 0.04–0.32; βCortisol/Cortisone 0.19; 95% CI: 0.08–0.31; β%Cortisol 0.12; 95% CI: 0.05–0.20). Gender differences suggest that the physiological response to precarious employment could be determined by the social construction of gender identities, as well as by positions and roles in the labour market and family. Future studies should delve further into these differences to improve employment and working policies, thus mitigating gender inequalities in the labour market to prevent work-related stress.
Trait Mindfulness and Physical Health among Chinese Middle-Older Adults: The Mediating Role of Mental Health
Authors: Lei Yuruo , Liu Jiawei , Wang Xinyu , Deng Zhiqi , Gao Qiufeng
Published: December 2022

Few studies have focused on the specific effects of trait mindfulness on physical health status, especially from a resilient aging perspective. This study examined the mediating role of mental health in the association between trait mindfulness and physical health status among middle-older adults in urban China. The participants included 188 individuals who were recruited from a community senior university and five community senior care centers. The findings reveal: (1) trait mindfulness has a strong effect on two physical health indicators (self-rated physical health and subjective sleep quality); (2) mental health is a significant mediator in the relationship between trait mindfulness and physical health status; and (3) the mediation role of mental health is more evident in the self-rated physical health model (24.15%) than subjective sleep quality (18.10%). This study improves our knowledge of how trait mindfulness can lead to a better physical health in middle-older adults and can lead to the development of social value communication and effective prevention.
The Influence of Freeze-Dried Alcohol-Water Extracts from Common Yarrow (Achillea millefolium L.) and German Chamomile (Matricaria chamomilla L.) on the Properties of Elastomer Vulcanizates
Authors: Aleksieiev Andrii , Masłowski Marcin , Efenberger-Szmechtyk Magdalena , Strzelec Krzysztof
Published: December 2022

This research work aimed to investigate the properties of freeze-dried extracts from Matricaria chamomilla L. and Achillea millefolium L. and to perform a characterization of their impact on the natural rubber-based vulcanizates. First, extracts were prepared in three different solvents at selected volume ratios: water (100), water-methanol (50/50), and water-ethanol (50/50). Next, the freeze-drying of extracts was established and then obtained bio-additives were introduced to the rubber mixtures. Freeze-dried extracts were investigated by UV-VIS diffuse reflectance spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Near-Infrared spectroscopy (NIR) and thermogravimetric analysis (TGA). Antioxidant activity and total phenolic content (TPC) were also defined. Rubber mixtures were examined in a rheometer and after vulcanization they were subjected to accelerated simulated aging by UV radiation and thermo-oxidative aging. To determine the resistance of vulcanizates to the degradation processes, the study of cross-linking density (equilibrium swelling method), mechanical properties (tensile strength, elongation at break) and color change were conducted. Performed studies proved the antioxidant activity of freeze-dried extracts caused by the high content of polyphenols and their beneficial influence on the properties of elastomer vulcanizates.
Protective Strategies of Haberlea rhodopensis for Acquisition of Freezing Tolerance: Interaction between Dehydration and Low Temperature
Authors: Georgieva Katya , Mihailova Gergana , Fernández-Marín Beatriz , Bertazza Gianpaolo , Govoni Annalisa , Arzac Miren Irati , Laza José Manuel , Vilas José Luis , García-Plazaola José Ignacio , Rapparini Francesca
Published: December 2022

Resurrection plants are able to deal with complete dehydration of their leaves and then recover normal metabolic activity after rehydration. Only a few resurrection species are exposed to freezing temperatures in their natural environments, making them interesting models to study the key metabolic adjustments of freezing tolerances. Here, we investigate the effect of cold and freezing temperatures on physiological and biochemical changes in the leaves of Haberlea rhodopensis under natural and controlled environmental conditions. Our data shows that leaf water content affects its thermodynamical properties during vitrification under low temperatures. The changes in membrane lipid composition, accumulation of sugars, and synthesis of stress-induced proteins were significantly activated during the adaptation of H. rhodopensis to both cold and freezing temperatures. In particular, the freezing tolerance of H. rhodopensis relies on a sucrose/hexoses ratio in favor of hexoses during cold acclimation, while there is a shift in favor of sucrose upon exposure to freezing temperatures, especially evident when leaf desiccation is relevant. This pattern was paralleled by an elevated ratio of unsaturated/saturated fatty acids and significant quantitative and compositional changes in stress-induced proteins, namely dehydrins and early light-induced proteins (ELIPs). Taken together, our data indicate that common responses of H. rhodopensis plants to low temperature and desiccation involve the accumulation of sugars and upregulation of dehydrins/ELIP protein expression. Further studies on the molecular mechanisms underlying freezing tolerance (genes and genetic regulatory mechanisms) may help breeders to improve the resistance of crop plants.
Cis-eQTL Analysis and Functional Validation of Candidate Genes for Carcass Yield Traits in Beef Cattle
Authors: Wang Tianzhen , Niu Qunhao , Zhang Tianliu , Zheng Xu , Li Haipeng , Gao Xue , Chen Yan , Gao Huijiang , Zhang Lupei , Liu George E. , Li Junya , Xu Lingyang
Published: December 2022

Carcass yield traits are of considerable economic importance for farm animals, which act as a major contributor to the world’s food supply. Genome-wide association studies (GWASs) have identified many genetic variants associated with carcass yield traits in beef cattle. However, their functions are not effectively illustrated. In this study, we performed an integrative analysis of gene-based GWAS with expression quantitative trait locus (eQTL) analysis to detect candidate genes for carcass yield traits and validate their effects on bovine skeletal muscle satellite cells (BSCs). The gene-based GWAS and cis-eQTL analysis revealed 1780 GWAS and 1538 cis-expression genes. Among them, we identified 153 shared genes that may play important roles in carcass yield traits. Notably, the identified cis-eQTLs of PON3 and PRIM2 were significantly (p < 0.001) enriched in previous GWAS loci for carcass traits. Furthermore, overexpression of PON3 and PRIM2 promoted the BSCs’ proliferation, increased the expression of MYOD and downregulated the expression of MYOG, which indicated that these genes may inhibit myogenic differentiation. In contrast, PON3 and PRIM2 were significantly downregulated during the differentiation of BSCs. These findings suggested that PON3 and PRIM2 may promote the proliferation of BSCs and inhibit them in the pre-differentiation stage. Our results further contribute to the understanding of the molecular mechanisms of carcass yield traits in beef cattle.
Expression of miRNA-Targeted and Not-Targeted Reporter Genes Shows Mutual Influence and Intercellular Specificity
Authors: Hudy Dorota , Rzeszowska-Wolny Joanna
Published: December 2022

The regulation of translation by RNA-induced silencing complexes (RISCs) composed of Argonaute proteins and micro-RNAs is well established; however, the mechanisms underlying specific cellular responses to miRNAs and how specific complexes arise are not completely clear. To explore these questions, we performed experiments with Renilla and firefly luciferase reporter genes transfected in a psiCHECK-2 plasmid into human HCT116 or Me45 cells, where only the Renilla gene contained sequences targeted by microRNAs (miRNAs) in the 3′UTR. The effects of targeting were miRNA-specific; miRNA-21-5p caused strong inhibition of translation, whereas miRNA-24-3p or Let-7 family caused no change or an increase in reporter Renilla luciferase synthesis. The mRNA-protein complexes formed by transcripts regulated by different miRNAs differed from each other and were different in different cell types, as shown by sucrose gradient centrifugation. Unexpectedly, the presence of miRNA targets on Renilla transcripts also affected the expression of the co-transfected but non-targeted firefly luciferase gene in both cell types. Renilla and firefly transcripts were found in the same sucrose gradient fractions and specific anti-miRNA oligoribonucleotides, which influenced the expression of the Renilla gene, and also influenced that of firefly gene. These results suggest that, in addition to targeted transcripts, miRNAs may also modulate the expression of non-targeted transcripts, and using the latter to normalize the results may cause bias. We discuss some hypothetical mechanisms which could explain the observed miRNA-induced effects.
Nanocomposites of Nitrogen-Doped Graphene Oxide and Manganese Oxide for Photodynamic Therapy and Magnetic Resonance Imaging
Authors: Khan Haseeb A. , Lee Yong-Kyu , Shaik Mohammed Rafi , Alrashood Sara T. , Ekhzaimy Aishah A.
Published: December 2022

Cancer is a leading cause of death worldwide. Conventional methods of cancer treatment, including chemotherapy and radiotherapy, are associated with multiple side effects. Recently, photodynamic therapy (PDT) has emerged as an effective therapeutic modality for cancer treatment without adversely affecting normal tissue. In this study, we synthesized nitrogen doped graphene (NDG) and conjugated it with Mn3O4 nanoparticles to produce NDG-Mn3O4 nanocomposite with the aim of testing its bimodal performance including PDT and magnetic resonance imaging (MRI). We did not use any linker or binder for conjugation between NDG and Mn3O4, rather they were anchored by a milling process. The results of cell viability analysis showed that NDG-Mn3O4 nanocomposites caused significant cell death under laser irradiation, while control and Mn3O4 nanoparticles showed negligible cell death. We observed increased generation of singlet oxygen after exposure of NDG-Mn3O4 nanocomposites, which was directly proportional to the duration of laser irradiation. The results of MRI showed concentration dependent enhancement of signal intensity with an increasing concentration of NDG-Mn3O4 nanocomposites. In conclusion, NDG-Mn3O4 nanocomposites did not cause any cytotoxicity under physiological conditions. However, they produced significant and dose-dependent cytotoxicity in cancer cells after laser irradiation. NDG-Mn3O4 nanocomposites also exhibited concentration-dependent MRI contrast property, suggesting their possible application for cancer imaging. Further studies are warranted to test the theranostic potential of NDG-Mn3O4 nanocomposites using animal models of cancer.
High-Performance Corrosion-Resistant Polymer/Graphene Nanomaterials for Biomedical Relevance
Authors: Kausar Ayesha , Ahmad Ishaq , Bocchetta Patrizia
Published: December 2022

Initially, pristine polymers were used to develop corrosion-resistant coatings. Later, the trend shifted to the use of polymeric nanocomposites in anti-corrosion materials. In this regard, graphene has been identified as an important corrosion-resistant nanomaterial. Consequently, polymer/graphene nanocomposites have been applied for erosion protection applications. Among polymers, conducting polymers (polyaniline, polypyrrole, polythiophene, etc.) and nonconducting polymers (epoxy, poly(methyl methacrylate), etc.) have been used as matrices for anticorrosion graphene nanocomposites. The corrosion-resistant polymer/graphene nanocomposites have found several important applications in biomedical fields such as biocompatible materials, biodegradable materials, bioimplants, tissue engineering, and drug delivery. The biomedical performance of the nanomaterials depends on the graphene dispersion and interaction with the polymers and living systems. Future research on the anti-corrosion polymer/graphene nanocomposite is desirable to perceive further advanced applications in the biomedical arenas.
High-Throughput Preparation and Characterization of ZrMoTaW Refractory Multi-Principal Element Alloy Film
Authors: Wang Qiannan , Yang Hongwang , Zuo Xiaojiao , Wang Yinxiao , Yao Jiahao
Published: December 2022

In this work, high-throughput screening technology is applied to four-member refractory multi-principal element alloys (RMPEAs) films with high W content. The exploration of refractory metals such as W is strictly limited by the high melting temperature in this work; a multi-gradient deposition method was introduced to overcome this obstacle. By adjusting the power and distance from the target to the sample, component Zr11Mo11Ta25W53 with the best hardening performance was successfully obtained. The uniformity of the material library was analyzed from the perspectives of phase structure and micromorphology. With the help of Hume-Rothery theory and XRD analysis, it is shown that the film has a stable bcc structure. It is believed that film uniformity, nanoscale size, preferential orientation, surface roughness, and solution mechanism are the pivotal factors to improve hardness performance, especially for high W components. The hardness and modulus of elasticity can reach 20 GPa and 300 GPa, respectively, and the H/Er and H3/Er2 values are 0.067 and 0.065, showing the best wear resistance in many samples.
Deep Tissue Characterization with Optical Coherence Elastography: A Comparison of Different Methods
Authors: Parmar Asha , Sharma Gargi , Ramming Andreas , Singh Kanwarpal
Published: December 2022

The measurement of the biomechanical properties of the skin is of great interest since these properties play an important role in the development of several diseases such as skin cancer and systemic sclerosis. In this direction, several diagnostic tools have been developed to analyze the mechanical properties of the skin. Optical coherence elastography (OCE) is one of the emerging imaging techniques used for the characterization of the mechanical properties of the tissue quantitatively. In systemic sclerosis patients, the measurement of the mechanical properties of the deeper skin layers is desirable compared to the superficial layers. There are several variants of OCE that exist, but it is still not clear which method is more suitable for the measurement of the mechanical properties of the deeper tissue. In this work, we tested three common methods, the pulsed excitation method, the continuous wave excitation method, and the resonant frequency method, for the measurement of the mechanical properties of the deeper layers in the tissue. We found out that the pulsed wave excitation method provides the most reliable measurements in the shortest possible time compared to the other two methods.