bluuebunny/crossref_metadata_2025 huggingface dataset:
New Accurate Flexural Analysis for Different Types of Plates in a Rectangular Sewage Tank by Utilizing a Unified Analytic Solution Procedure
Authors: Sun Guangxi , Zhang Gang , Huang Jianrong , Shi Qiaoli , Tang Xiaocheng , Ullah Salamat
Published: April 2024

In the present paper, a modified Fourier series approach is developed for new precise flexural analysis of three different types of concrete plates in a rectangular sewage tank. The bending problems of the bottom plate, side-plate, and the fluid-guiding plate are not easily solved via using the traditional analytic approaches. Based on the Fourier series theory, the present approach provides a unified semi-inverse solving procedure for the above plates by means of choosing three different kinds of Fourier series as the trial functions. Although all the trial functions are quite similar to the classical Navier-form solution, new, precise analytic flexural solutions for plates without Navier-type edge conditions (all edges simply-supported) are achieved, which is mainly attributed to employing the Stoke’s transform technique. For each case, the plate-bending problems are finally altered to deal with linear algebra equations. Furthermore, owing to the orthogonality and completeness of the Fourier series, the obtained solutions perfectly satisfy both the edge conditions and the governing partial differential equation of plates, which paves an easily implemented and rational way for engineers and researchers to provide new, exact designs of plate structures. The main contribution of this study lies in the provision of a unified solution procedure for addressing complex plate-bending problems across diverse boundary conditions. By employing a range of Fourier series types, this approach offers a comprehensive solution framework that accommodates the complexities inherent in plate analysis. The correctness of the present analytic solutions is verified against precise finite element method (FEM) results and ones available in the literature. Finally, the influences of foundation, edge conditions, and aspect ratio on flexural behaviors of plates are discussed in detail.
Biphasic Regulation of Apoptosis Following Gastric Irreversible Electroporation Using Tissue Immunohistochemistry of Activated Caspase-3 with TUNEL Method
Authors: Jeon Han Jo , Chun Hoon Jai , Choi Hyuk Soon , Keum Bora , Kim Hong Bae , Kim Jong Hyuk
Published: April 2024

The regulation of apoptosis is the primary goal of ablation therapy. Irreversible electroporation (IRE) is a promising non-thermal tissue ablation-based therapy that induces apoptosis by manipulating electrical conditions. This study aimed to investigate IRE-induced gastric tissue apoptosis in response to changes in the electric field intensity, followed by the repair process. Among the 52 rats used in this study, 24 were used to explore apoptosis, and 28 were used to study regeneration. The apoptosis-to-necrosis ratio of the electrical field strength was evaluated using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling and caspase-3 immunohistochemistry. The size of IRE-induced ulcers in the gastric tissue continuously increased with increasing electrical intensity (r2 = 0.830, p < 0.001). The level of apoptosis gradually decreased after peaking at 200 V (1000 V/cm). The size of the 400 V-ablated ulcers continued to decrease, and they were not visible by day 14. The proliferation and migration of epithelial cells with fibroblasts were observed on day 3 and augmented on day 7 post-ablation. This investigation demonstrated the biphasic activation of apoptosis with respect to the electrical field strength. Visually and histologically, IRE-induced gastric ulcers demonstrated complete tissue regeneration after two weeks.
Challenges and Considerations of Preclinical Development for iPSC-Based Myogenic Cell Therapy
Authors: Sun Congshan , Serra Carlo , Kalicharan Brianna Harley , Harding Jeffrey , Rao Mahendra
Published: April 2024

Cell therapies derived from induced pluripotent stem cells (iPSCs) offer a promising avenue in the field of regenerative medicine due to iPSCs’ expandability, immune compatibility, and pluripotent potential. An increasing number of preclinical and clinical trials have been carried out, exploring the application of iPSC-based therapies for challenging diseases, such as muscular dystrophies. The unique syncytial nature of skeletal muscle allows stem/progenitor cells to integrate, forming new myonuclei and restoring the expression of genes affected by myopathies. This characteristic makes genome-editing techniques especially attractive in these therapies. With genetic modification and iPSC lineage specification methodologies, immune-compatible healthy iPSC-derived muscle cells can be manufactured to reverse the progression of muscle diseases or facilitate tissue regeneration. Despite this exciting advancement, much of the development of iPSC-based therapies for muscle diseases and tissue regeneration is limited to academic settings, with no successful clinical translation reported. The unknown differentiation process in vivo, potential tumorigenicity, and epigenetic abnormality of transplanted cells are preventing their clinical application. In this review, we give an overview on preclinical development of iPSC-derived myogenic cell transplantation therapies including processes related to iPSC-derived myogenic cells such as differentiation, scaling-up, delivery, and cGMP compliance. And we discuss the potential challenges of each step of clinical translation. Additionally, preclinical model systems for testing myogenic cells intended for clinical applications are described.
A Qualitative Study of Preschool Children’s Perspectives on an Oral Health Promotion Program in New South Wales, Australia
Authors: Parmar Jinal Shashin , Sanagavarapu Prathyusha , Micheal Sowbhagya , Chandio Navira , Cartwright Susan , Arora Amit
Published: April 2024

In Australia, dental caries are observed in almost half of children starting school. Oral health promotion programs are being implemented in early childhood education and care (ECEC) settings to promote oral health. This study examined children’s perceptions of one such program, the Bright Smiles Bright Futures (BSBF) program in ECEC settings in New South Wales, Australia. Data were collected using focus group discussions from 15 children aged 3–5 years, transcribed verbatim, and analysed through inductive thematic analysis. Three themes were identified as follows: (i) oral health knowledge of children, (ii) oral hygiene practices routine and skills development, and (iii) evaluation of the oral health promotion kit and opportunities for improvement. Children’s perspectives highlight the BSBF program’s success in communicating key messages to promote oral health. The integration of family-centric approaches, acknowledgement of children’s preferences, and the use of interactive tools collectively enhance the overall effectiveness of the oral health promotion program.
Endowing Ferroelectric Properties of Tetragonal Lysozyme Crystals through C60 Doping
Authors: Zhou Renbin , Liu Xuejiao , Guo Weihong , Yin Dachuan
Published: April 2024

The inherent nonpolarity of tetragonal lysozyme crystals excludes a ferroelectricity response. Herein, we present a demonstration of achieving measurable ferroelectricity in tetragonal lysozyme crystals through C60 doping. Ferroelectric characterizations revealed that C60-doped tetragonal lysozyme crystals exhibited typical characteristic ferroelectric hysteresis loops. Crystallographic structural analysis suggested that C60 doping may induce a reduction in the overall symmetry of tetragonal Lys@C60, leading to the observed ferroelectricity response. Moreover, the introduction of C60 facilitates efficient electron transport inside the crystal and influences the polarization of Lys@C60, further contributing to the observed ferroelectricity response. This work verifies that C60 doping can serve as a simple strategy to bestow novel ferroelectric properties to non-ferroelectric lysozyme crystals, potentially rendering them suitable for biocompatible and biodegradable application in implantable and wearable bioelectronics.
Prospective Cohort Study Identifies Medical Predictors of Treatment-Related Oral Toxicities in Oral and Oropharyngeal Cancer Patients
Authors: Rodrigues-Oliveira Leticia , Rivera César , López-Cortés Xaviera A. , Mak Milena Perez , Mores Ana Leticia , Migliorati Cesar Augusto , Querido de Oliveira Maria Cecília , Palmier Natalia Rangel , Gueiros Luiz Alcino , Vargas Pablo Agustin , Brandão Thaís Bianca , Santos-Silva Alan Roger , Prado-Ribeiro Ana Carolina
Published: April 2024

The dental treatment of patients with oral cavity and oropharyngeal squamous cell carcinoma (OOPSCC) may be challenging for dentists. This study aimed to characterize systemic changes in patients with OOPSCC undergoing dental treatment prior to cancer therapy, with a specific focus on laboratory assessments. The primary objectives included identifying potential adverse events, such as infections or bleeding, resulting from dental procedures. Additionally, the study aimed to correlate baseline patient characteristics with treatment-related toxicities. This was a prospective cohort study that included 110 OOPSCC patients referred to the Dental Oncology Service at São Paulo State Cancer Institute, Brazil, between November/2019 and December/2020. Comorbidities, sociodemographic data, medication in use, cancer treatment-related toxicities, and altered laboratory tests results were correlated. The most common comorbidities and altered laboratory results were hypertension, dyslipidemia, diabetes, as well as elevated levels of C-reactive protein, hemoglobin, and hematocrit. Toxicities exhibited a progressive pattern over time, encompassing oral mucositis (OM), xerostomia, dysphagia, dysgeusia, trismus, and radiodermatitis. No correlation between comorbidities and cancer treatment-related toxicities, a positive correlation between medications in use and OM, and a negative correlation between medications and dysgeusia were found. OM was associated with altered thyroxine (T4) and free thyroxine (FT4), calcium, urea, creatinine, alkaline phosphatase, and syphilis. Family income and housing were OM predictors. Altered T4/FT4/urea/calcium/alkaline phosphatase/creatinine/syphilis may be useful clinical predictors of OM. Despite the elevated prevalence of comorbidities and abnormal laboratory findings, dental treatment prior to cancer treatment yielded no adverse events.
Development and Testing of the Aftercare Problem List, a Burn Aftercare Screening Instrument
Authors: Van Loey Nancy E. E. , Boersma-van Dam Elise , Boekelaar Anita , van de Steenoven Anneke , de Jong Alette E. E. , Hofland Helma W. C.
Published: April 2024

A growing interest in person-centered care from a biopsychosocial perspective has led to increased attention to structural screening. The aim of this study was to develop an easy-to-comprehend screening instrument using single items to identify a broad range of health-related problems in adult burn survivors. This study builds on earlier work regarding content generation. Focus groups and expert meetings with healthcare providers informed content refinement, resulting in the Aftercare Problem List (APL). The instrument consists of 43 items divided into nine health domains: scars, daily life functioning, scars treatment, body perceptions, stigmatization, intimacy, mental health, relationships, financial concerns, and a positive coping domain. The APL also includes a Distress Thermometer and a question inquiring about preference to discuss the results with a healthcare provider. Subsequently, the APL was completed by 102 outpatients. To test face validity, a linear regression analysis showed that problems in three health domains, i.e., scars, mental health, and body perceptions, were significantly related to higher distress. Qualitative results revealed that a minority found the items difficult which led to further adjustment of the wording and the addition of illustrations. In summation, this study subscribes to the validity of using single items to screen for burn-related problems.
Two-Dimensional Protection Code for Virtual Page Information in Translation Lookaside Buffers
Authors: Gao Xin , Cui Naiyuan , Nian Jiawei , Liu Hongjin , Yang Mengfei
Published: April 2024

Severe conditions such as high-energy particle strikes may induce soft errors in on-chip memory, like cache and translation lookaside buffers (TLBs). As the key component of virtual-to-physical address translation, TLB directly affects processor performance. To protect the virtual page information stored in TLB, several studies have introduced error detection or correction codes. However, most schemes proposed for data TLB cannot support the detection of multi-bit upsets, which is reported as a serious issue in modern fault-tolerant processors due to the downscaling of CMOS process technology. In this paper, we propose a new two-dimensional protection technique, called the matrix protection tag (MaP-Tag), to provide stronger error detection and correction capability to TLB virtual page information. Our proposal reorganizes virtual page information into a matrix and employs adjacent check bits and interleaved check bits for error detection. The two-dimensional design offers one-bit error detection, burst multi-bit error detection, and even multi-bit error correction in some cases. The simulation results show that our proposal can detect almost all error patterns when injected with up to eight bit-flips. Furthermore, the technique provides a better error correction rate than conventional single error correction (SEC) codes. The reliability calculation shows that our proposal is powerful in both error detection and correction with affordable storage overhead.
Experimental Study of Magnetron’s Power-Pulled Characteristic to Realize a Quasi-Dual-Frequency Microwave Output
Authors: Zhao Yan , Wang Shaoyue , He Zhongqi , Yan Liping , Liu Changjun
Published: April 2024

This article presents experimental evidence demonstrating the feasibility of implementing a quasi-dual-frequency microwave signal generator using a 1 kW commercial magnetron. The injection-pulled magnetron may generate quasi-dual-frequency output with nearly identical amplitudes by injecting signal at specific powers and frequencies out of the Adler locking band. This generator offers the advantage of developing a large-power and tunable quasi-dual-frequency signal at low cost and with simple implementation. In the present study, a tunable quasi-dual-frequency signal with a frequency interval ranging from 0.48 MHz to 13.74 MHz is generated experimentally. Furthermore, the effects of anode voltage ripples on the dual-frequency outputs are experimentally investigated. An empirical relationship between the frequency interval and injection ratio is obtained and presented. In addition, neither the output power nor the efficiency of the magnetron in the dual-frequency mode decreases. This research is promising for improving microwave heating uniformity in large-scale industrial applications.
Combined Shark-Fin Rooftop Antenna for LTE, WLAN and BeiDou Applications
Authors: Shen Lingrong , Luo Wei , Miao Youming , Liu Gui
Published: April 2024

This paper presents rooftop automobile antennas designed for Long-Term Evolution (LTE), Wireless Local Area Network (WLAN) and navigation system applications. The proposed antennas are housed within a shark-fin structure on the car’s roof, and comprise a main antenna and a diversity antenna. The main antenna and diversity antenna combine for spatial diversity, receiving and processing the same signal to optimize signal quality. To accommodate the limited space within the shark-fin housing, various miniaturization and multiband techniques are utilized. The hexagonal substrate is more closely fitted to the shape of the shark fin, thus making full use of the space of the shark-fin shell. The main antenna and the diversity antenna are perpendicular to each other, which saves the space of the overall antenna and improves the utilization rate of the overall antenna space. The proposed main antenna, compactly sized at 50 mm × 20 mm × 1.59 mm, maintains a VSWR value below 2 across the frequency range of 1.19–2.8 GHz, enabling support for LTE bands 1, 2, 3, 4, 7, 11, 15, 16, 34, 39, 40, and 41, as well as WLAN 2400 bands. The diversity antenna maintains a VSWR value below 2 across the frequency range of 1.5–2.6 GHz, which can cover BeiDou B1-1, LTE 1, 2, 3, 4, 7, 11, 15, 16, 34, 39, and 40, and WLAN 2400 bands. The main antenna and the diversity antenna both demonstrate favorable radiation patterns on the azimuth plane. Simulation and measurement results exhibit a high level of agreement.
Innovative Firmware Update Method to Microcontrollers during Runtime
Authors: Neves Bernardino Pinto , Santos Victor D. N. , Valente António
Published: April 2024

This article presents a new firmware update paradigm for optimising the procedure in microcontrollers. The aim is to allow updating during program execution, without interruptions or restarts, replacing only specific code segments. The proposed method uses static and absolute addresses to locate and isolate the code segment to be updated. The work focuses on Microchip’s PIC18F27K42 microcontroller and includes an example of updating functionality without affecting ongoing applications. This approach is ideal for band limited channels, reducing the amount of data transmitted during the update process. It also allows incremental changes to the program code, preserving network capacity, and reduces the costs associated with data transfer, especially in firmware update scenarios using cellular networks. This ability to update the normal operation of the device, avoiding service interruption and minimising downtime, is of remarkable value.
Digital Traceability in Agri-Food Supply Chains: A Comparative Analysis of OECD Member Countries
Authors: Charlebois Sylvain , Latif Noor , Ilahi Ibrahim , Sarker Bibhuti , Music Janet , Vezeau Janele
Published: April 2024

In an era marked by globalization and rapid technological advancements, the agri-food sector confronts both unprecedented challenges and opportunities. Among these, digital traceability systems have emerged as pivotal in enhancing operational efficiencies, ensuring food safety, and promoting transparency throughout the supply chain. This study presents a comparative analysis of digital traceability adoption and its impact across member countries of the Organization for Economic Co-operation and Development (OECD). By utilizing a multidimensional analytical framework, this study investigates national regulations, legal frameworks, and key food commodities affected by digital traceability implementations. It systematically assesses the efficacy of these systems in meeting consumer transparency expectations, regulatory compliance, and the overarching goal of sustainable agri-food supply chains. Through case studies and empirical evidence, the paper elucidates the complex interplay between technological innovation and regulatory environments, offering insights into best practices and potential integration barriers. Ultimately, this comprehensive investigation contributes to the scholarly discourse on digital traceability, providing actionable recommendations for policymakers, industry stakeholders, and academia to navigate the complexities of modern agri-food systems.
Differences in Biofilm Formation of Listeria monocytogenes and Their Effects on Virulence and Drug Resistance of Different Strains
Authors: Yang Yujuan , Kong Xiangxiang , Niu Bing , Yang Jielin , Chen Qin
Published: April 2024

Listeria monocytogenes is recognized as one of the primary pathogens responsible for foodborne illnesses. The ability of L. monocytogenes to form biofilms notably increases its resistance to antibiotics such as ampicillin and tetracycline, making it exceedingly difficult to eradicate. Residual bacteria within the processing environment can contaminate food products, thereby posing a significant risk to public health. In this study, we used crystal violet staining to assess the biofilm-forming capacity of seven L. monocytogenes strains and identified ATCC 19112 as the strain with the most potent biofilm-forming. Subsequent fluorescence microscopy observations revealed that the biofilm-forming capacity was markedly enhanced after two days of culture. Then, we investigated into the factors contributing to biofilm formation and demonstrated that strains with more robust extracellular polymer secretion and self-agglutination capabilities exhibited a more pronounced ability to form biofilms. No significant correlation was found between surface hydrophobicity and biofilm formation capability. In addition, we found that after biofilm formation, the adhesion and invasion of cells were enhanced and drug resistance increased. Therefore, we hypothesized that the formation of biofilm makes L. monocytogenes more virulent and more difficult to remove by antibiotics. Lastly, utilizing RT-PCR, we detected the expression levels of genes associated with biofilm formation, including those involved in quorum sensing (QS), flagellar synthesis, and extracellular polymer production. These genes were significantly upregulated after biofilm formation. These findings underscore the critical relationship between extracellular polymers, self-agglutination abilities, and biofilm formation. In conclusion, the establishment of biofilms not only enhances L. monocytogenes’ capacity for cell invasion and adhesion but also significantly increases its resistance to drugs, presenting a substantial threat to food safety.
Determination of Multiclass Antibiotics in Fish Muscle Using a QuEChERS-UHPLC-MS/MS Method
Authors: Aissaoui Yousra , Jiménez-Skrzypek Gabriel , González-Sálamo Javier , Trabelsi-Ayadi Malika , Ghorbel-Abid Ibtissem , Hernández-Borges Javier
Published: April 2024

The surging global demand for fish has increased aquaculture practices, where antibiotics have become indispensable to prevent diseases. However, the passive incorporation of these compounds into the diet may have adverse effects on human health. In this work, the QuEChERS method combined with ultra-high-performance liquid chromatography tandem mass spectrometry was applied for the determination of 10 multiclass antibiotics (5 quinolones, 2 sulfonamides, 2 diaminopyrimidines, and 1 macrolide) in muscle tissue of farmed fish (European sea bass and gilt-head sea bream). The applied method demonstrated acceptable recovery values, mostly between 70 and 120%, with limits of quantification of the method meeting the established EU maximum residue limits. The analysis of twenty fish samples in duplicate revealed that most antibiotics were not present, with the only exception of oxolinic acid and tilmicosin in European sea bass, which were below the limit of quantification of the method.
Characterization of Genomic, Physiological, and Probiotic Features of Lactiplantibacillus plantarum JS21 Strain Isolated from Traditional Fermented Jiangshui
Authors: Liu Yang , Wang Shanshan , Wang Ling , Lu Hongzhao , Zhang Tao , Zeng Wenxian
Published: April 2024

This study aimed to understand the genetic and metabolic traits of a Lactiplantibacillus plantarum JS21 strain and its probiotic abilities through laboratory tests and computer analysis. L. plantarum JS21 was isolated from a traditional fermented food known as “Jiangshui” in Hanzhong city. In this research, the complete genetic makeup of JS21 was determined using Illumina and PacBio technologies. The JS21 genome consisted of a 3.423 Mb circular chromosome and five plasmids. It was found to contain 3023 protein-coding genes, 16 tRNA genes, 64 rRNA operons, 40 non-coding RNA genes, 264 pseudogenes, and six CRISPR array regions. The GC content of the genome was 44.53%. Additionally, the genome harbored three complete prophages. The evolutionary relationship and the genome collinearity of JS21 were compared with other L. plantarum strains. The resistance genes identified in JS21 were inherent. Enzyme genes involved in the Embden–Meyerhof–Parnas (EMP) and phosphoketolase (PK) pathways were detected, indicating potential for facultative heterofermentative pathways. JS21 possessed bacteriocins plnE/plnF genes and genes for polyketide and terpenoid assembly, possibly contributing to its antibacterial properties against Escherichia coli (ATCC 25922), Escherichia coli (K88), Staphylococcus aureus (CMCC 26003), and Listeria monocytogenes (CICC 21635). Furthermore, JS21 carried genes for Na+/H+ antiporters, F0F1 ATPase, and other stress resistance genes, which may account for its ability to withstand simulated conditions of the human gastrointestinal tract in vitro. The high hydrophobicity of its cell surface suggested the potential for intestinal colonization. Overall, L. plantarum JS21 exhibited probiotic traits as evidenced by laboratory experiments and computational analysis, suggesting its suitability as a dietary supplement.
Crater–Spectrum Feature Fusion Method for Panax notoginseng Cadmium Detection Using Laser-Induced Breakdown Spectroscopy
Authors: Chen Rongqin , Li Xiaolong , Li Weijiao , Yang Rui , Lu Yi , You Zhengkai , Liu Fei
Published: April 2024

Panax notoginseng (P. notoginseng) is a valuable herbal medicine, as well as a dietary food supplement known for its satisfactory clinical efficacy in alleviating blood stasis, reducing swelling, and relieving pain. However, the ability of P. notoginseng to absorb and accumulate cadmium (Cd) poses a significant environmental pollution risk and potential health hazards to humans. In this study, we employed laser-induced breakdown spectroscopy (LIBS) for the rapid detection of Cd. It is important to note that signal uncertainty can impact the quantification performance of LIBS. Hence, we proposed the crater–spectrum feature fusion method, which comprises ablation crater morphology compensation and characteristic peak ratio correction (CPRC), to explore the feasibility of signal uncertainty reduction. The crater morphology compensation method, namely, adding variables using multiple linear regression (MLR) analysis, decreased the root-mean-square error of the prediction set (RMSEP) from 7.0233 μg/g to 5.4043 μg/g. The prediction results were achieved after CPRC pretreatment using the calibration curve model with an RMSEP of 3.4980 μg/g, a limit of detection of 1.92 μg/g, and a limit of quantification of 6.41 μg/g. The crater–spectrum feature fusion method reached the lowest RMSEP of 2.8556 μg/g, based on a least-squares support vector machine (LSSVM) model. The preliminary results suggest the effectiveness of the crater–spectrum feature fusion method for detecting Cd. Furthermore, this method has the potential to be extended to detect other toxic metals in addition to Cd, which significantly contributes to ensuring the quality and safety of agricultural production.
Metabolomics Combined with Correlation Analysis Revealed the Differences in Antioxidant Activities of Lotus Seeds with Varied Cultivars
Authors: Yu Xinjin , Wang Yuting , Yan Xiaoli , Leng Tuo , Xie Jianhua , Yu Qiang , Chen Yi
Published: April 2024

Functional foods have potential health benefits for humans. Lotus seeds (LS) as functional foods have excellent antioxidant activities. However, the differences in chemical composition of different LS cultivars may affect their antioxidant activities. This study comprehensively analyzed the differences among five LS cultivars based on metabolomics and further revealed the effects of metabolites on antioxidant activities by correlation analysis. A total of 125 metabolites were identified in LS using UPLC-Q/TOF-MS. Then, 15 metabolites were screened as differential metabolites of different LS cultivars by chemometrics. The antioxidant activities of LS were evaluated by DPPH•, FRAP, and ABTS•+ assays. The antioxidant activities varied among different LS cultivars, with the cultivar Taikong 66 showing the highest antioxidant activities. The correlation analysis among metabolites and antioxidant activities highlighted the important contribution of phenolics and alkaloids to the antioxidant activities of LS. Particularly, 11 metabolites such as p-coumaric acid showed significant positive correlation with antioxidant activities. Notably, 6 differential metabolites screened in different LS cultivars showed significant effects on antioxidant activities. These results revealed the important effects of phytochemicals on the antioxidant activities of different LS cultivars. This study provided evidence for the health benefits of different LS cultivars.
Carbon Footprint of Oxygenated Gasolines: Case Studies in Latin America, Asia, and Europe
Authors: Koupal John , Cashman Sarah , Young Ben , Henderson Andrew D.
Published: April 2024

Lifecycle analysis was used to estimate well-to-wheel greenhouse gas (GHG) emissions associated with the production, transport, and use of oxygenated gasoline in Colombia, Japan, and France. The study evaluated fuel blends containing ethanol and/or ethyl tertiary-butyl ether (ETBE) that aligned with oxygen and octane specifications currently in place or under consideration for each country. For Colombia, fuel blends meeting a 3.7 wt.% oxygen specification were analyzed using ethanol sourced and produced in the U.S. from corn and in Colombia from sugarcane, and ETBE processed in the U.S. Gulf Coast. For Japan, blends with 1.3, 2.7 and 3.7 wt.% oxygen were analyzed using ethanol sourced and produced in the U.S. and Brazil, and ETBE processed in the U.S. Gulf Coast. For France, oxygenated gasoline blends with 3.7 to 8.0 wt.% oxygen content were analyzed with ethanol produced locally from corn, beet, and wood and imported sugarcane ethanol. Data were populated from both publicly available secondary data sources and new primary data developed for ETBE production in the U.S. and Europe. This study also accounted for distinct lifecycle emissions among gasoline components, focused on aromatic-rich reformate used to boost octane in non-oxygenated fuels. Across each country, results indicate that the replacement of reformate in ethanol-free (E0) gasoline with oxygenates up to 3.7 wt.% oxygen reduces lifecycle GHG emissions by 6–9%, with the highest GHG reduction provided when ETBE alone is used for oxygenate. For higher oxygen blends modeled for France, the highest GHG reduction (19%) was for a blend of 51 vol.% ETBE to achieve 8.0 wt.% oxygen, the equivalent of E23 (gasoline with 23 vol.% ethanol). Overall, displacing ethanol with ETBE to achieve a fixed oxygen level increased GHG benefits relative to ethanol-only blends, owing to the greater volume of the carbon-intensive reformate displaced.
A Lignin Silver Nanoparticles/Polyvinyl Alcohol/Sodium Alginate Hybrid Hydrogel with Potent Mechanical Properties and Antibacterial Activity
Authors: Yu Jie , Ran Fangli , Li Chenyu , Hao Zhenxin , He Haodong , Dai Lin , Wang Jingfeng , Yang Wenjuan
Published: April 2024

Antibacterial hydrogels have attracted significant attention due to their diverse applications, efficient antimicrobial properties, and adaptability to various environments and requirements. However, their relatively fragile structure, coupled with the potential for environmental toxicity when exposed to their surroundings for extended periods, may significantly limit their practical application potential. In this work, a composite hydrogel was synthesized with outstanding mechanical features and antibacterial capability. The hydrogel was developed through the combination of the eco-friendly and enduring antibacterial agent, lignin silver nanoparticles (Lig-Ag NPs), with polyvinyl alcohol (PVA) and sodium alginate (SA), in varying proportions. The successful synthesis of the hydrogel and the dispersed distribution of Lig-Ag NPs within the hydrogel were confirmed by various analytical techniques, including field emission scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), mercury intrusion porosimetry (MIP), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The formation of multiple hydrogen bonds between Lig-Ag NPs and the composites contributed to a more stable and dense network structure of the hydrogel, consequently enhancing its mechanical properties. Rheological tests revealed that the hydrogel exhibited an elastic response and demonstrated outstanding self-recovery properties. Significantly, the antibacterial hydrogel demonstrated effectiveness against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), achieving a <5% survival of bacteria within 12 h. This study presented a green and straightforward synthetic strategy for the application of antibacterial composite hydrogels in various fields.
Sulfur Impurities: The Overlooked Process in Volcanic Hazard Assessment
Authors: Scolamacchia Teresa
Published: April 2024

One of the most intriguing questions of modern volcanology is the inception of an eruption. Despite efforts to detect premonitory signals, numerous unpredicted eruptions have occurred recently. It has been suggested that these unpredicted eruptions might be explained by viscosity variations in elemental sulfur accumulated within the hydrothermal systems present in several volcanic settings under the influence of organics, hydrocarbons, hydrogen sulfide, halogens, and ammonia. Changes in impure sulfur viscosity are more complex than those in pure S, invoked decades ago to trigger eruptions by system sealing in volcanoes hosting a crater lake. Growing evidence suggests that sulfur accumulation is a common process, not restricted to crater lakes. Moreover, both types and amounts of gas species released at the surface, critical for volcano monitoring, would be altered, following chemical reactions involving impure S, invalidating signals used to issue alerts. Impure sulfur behavior may explain puzzling degassing and contrasting signals reported at volcanoes and restless calderas worldwide, with implications for hazard assessment and volcanic-risk-mitigation strategies.
The Time Trajectory of Choroid Plexus Enlargement in Multiple Sclerosis
Authors: Andravizou Athina , Stavropoulou De Lorenzo Sotiria , Kesidou Evangelia , Michailidou Iliana , Parissis Dimitrios , Boziki Marina-Kleopatra , Stamati Polyxeni , Bakirtzis Christos , Grigoriadis Nikolaos
Published: April 2024

Choroid plexus (CP) can be seen as a watchtower of the central nervous system (CNS) that actively regulates CNS homeostasis. A growing body of literature suggests that CP alterations are involved in the pathogenesis of multiple sclerosis (MS) but the underlying mechanisms remain elusive. CPs are enlarged and inflamed in relapsing-remitting (RRMS) but also in clinically isolated syndrome (CIS) and radiologically isolated syndrome (RIS) stages, far beyond MS diagnosis. Increases in the choroid plexus/total intracranial volume (CP/TIV) ratio have been robustly associated with increased lesion load, higher translocator protein (TSPO) uptake in normal-appearing white matter (NAWM) and thalami, as well as with higher annual relapse rate and disability progression in highly active RRMS individuals, but not in progressive MS. The CP/TIV ratio has only slightly been correlated with magnetic resonance imaging (MRI) findings (cortical or whole brain atrophy) and clinical outcomes (EDSS score) in progressive MS. Therefore, we suggest that plexus volumetric assessments should be mainly applied to the early disease stages of MS, whereas it should be taken into consideration with caution in progressive MS. In this review, we attempt to clarify the pathological significance of the temporal CP volume (CPV) changes in MS and highlight the pitfalls and limitations of CP volumetric analysis.
Elderly Patients with Newly Diagnosed Multiple Myeloma: Continuous or Fixed Duration Treatment?
Authors: Manier Salomon , Facon Thierry
Published: April 2024

Multiple myeloma (MM) presents unique challenges in the elderly population due to increased frailty and comorbidities. Balancing treatment efficacy, safety, and quality of life is essential in managing elderly patients. While two-drug regimens were often favored for elderly patients, recent studies show promising outcomes with anti-CD38 antibody-based therapies, particularly daratumumab and lenalidomide with minimal dexamethasone. Continuous low-intensity treatments have shown improved progression-free survival and overall survival, with significant benefits observed in elderly patients. The DRd combination has now emerged as the standard of care for elderly MM patients, offering a favorable balance of efficacy, safety, and convenience. Ongoing trials are evaluating the addition of bortezomib in an induction phase for fit patients. New-generation immunotherapies hold promise for further refining treatment approaches, potentially leading to treatment discontinuation in select patient populations with sustained minimal residual disease negativity.
Proto-Early Renaissance Depictions, Iconographic Analysis and Computerised Facial Similarity Assessment Connections: The 16th Century Mural Paintings of St. Leocadia Church (Chaves, North of Portugal)
Authors: Salavessa Eunice , Aranha José , Moreira Rafael , Freire-Lista David M.
Published: April 2024

The aim of this paper is to analyse facial similarity and apply it to identify the individuals depicted in the mural paintings of the apse of St. Leocadia Church, located in Chaves Municipality (North of Portugal), which were painted during the first quarter of the 16th century. This study also compares the portraits of this mural paintings with the oil paintings by the Proto-Renaissance Portuguese painter Nuno Gonçalves. Through this research, the feasibility of face recognition technology is explored to answer many ambiguities about Manueline stylistic identity and iconography. Additionally, it aims to associate historical events, artistic discoveries, and the expansion of portraiture as propaganda of power during the Portuguese Proto-Renaissance and Early Renaissance. On the other hand, it focuses on the prevalence of the religious and devotional over the sacred in Manueline painting. A proposal was made to identify the characters that are fundamental to the meaning of the mural paintings. An experiment was conducted on seven characters from the paintings at St. Leocadia Church, which were then compared to Nuno Gonçalves’ portraits. Facial similarity analysis was conducted on the faces portrayed in the Panels of St. Vincent, a remarkable portrait gallery from 15th-century Portugal, which has been the subject of national and international research for 130 years. Other paintings that were analysed were the oil paintings of St. Peter and St. Paul and of Infanta St. Joana, which were created by the same Quattrocento master. The purpose of the mural paintings of St. Leocadia Church could be catechetical in nature or related to the ritual practices of royal ancestor worship in royal portrait apses of the churches. It could also be associated with the Portuguese maritime expansion and the macro-imperial ideology of D. Manuel I.
Respiratory Syncytial Virus Infections in Recipients of Bone Marrow Transplants: A Systematic Review and Meta-Analysis
Authors: Riccò Matteo , Parisi Salvatore , Corrado Silvia , Marchesi Federico , Bottazzoli Marco , Gori Davide
Published: April 2024

Human Respiratory Syncytial Virus (RSV) is a common cause of respiratory tract infections. Usually associated with infants and children, an increasing amount of evidence suggests that RSV can cause substantial morbidity and mortality in immunocompromised individuals, including recipients of bone marrow transplantation (BMT). The present systematic review was therefore designed in accordance with the PRISMA guidelines to collect available evidence about RSV infections in BMT recipients. Three medical databases (PubMed, Embase, and MedRxiv) were therefore searched for eligible observational studies published up to 30 September 2023 and collected cases were pooled in a random-effects model. Heterogeneity was assessed using I2 statistics. Reporting bias was assessed by means of funnel plots and regression analysis. Overall, 30 studies were retrieved, including 20,067 BMT cases and 821 RSV infection episodes. Of them, 351 were lower respiratory tract infections, and a total of 78 RSV-related deaths were collected. A pooled attack rate of 5.40% (95% confidence interval [95%CI] 3.81 to 7.60) was identified, with a corresponding incidence rate of 14.77 cases per 1000 person-years (95%CI 9.43 to 20.11), and a case fatality ratio (CFR) of 7.28% (95%CI 4.94 to 10.60). Attack rates were higher in adults (8.49%, 95%CI 5.16 to 13.67) than in children (4.79%, 95%CI 3.05 to 7.45), with similar CFR (5.99%, 95%CI 2.31 to 14.63 vs. 5.85%, 95%CI 3.35 to 10.02). By assuming RSV attack rates as a reference group, influenza (RR 0.518; 95%CI 0.446 to 0.601), adenovirus (RR 0.679, 95%CI 0.553 to 0.830), and human metapneumovirus (RR 0.536, 95%CI 0.438 to 0.655) were associated with a substantially reduced risk for developing corresponding respiratory infection. Despite the heterogeneous settings and the uneven proportion of adult and pediatric cases, our study has identified high attack rates and a substantial CFR of RSV in recipients of BMT, stressing the importance of specifically tailored preventive strategies and the need for effective treatment options.
The Effect of Combining Blood Flow Restriction with the Nordic Hamstring Exercise on Hamstring Strength: Randomized Controlled Trial
Authors: Pişkin Necdet Eray , Yavuz Gönül , Aktuğ Zait Burak , Aldhahi Monira I. , Al-Mhanna Sameer Badri , Gülü Mehmet
Published: April 2024

(1) Background: It is a matter of curiosity what effect the blood flow restriction (BFR) method, which is usually combined with low-intensity resistance exercises, will have when used with high-intensity eccentric exercises. (2) Methods: The present study examined the effects of combining BFR with nordic hamstring exercises (NHEs) on hamstring muscle strength, bilateral deficit (BLD), and training volume. Thirty young female volleyball players, who trained three times a week, participated voluntarily in the study. These players were stratified into three groups, each comprising ten individuals: a control group (CG), an NHE group, and an NHE + BFR group. Hamstring muscle strength and BLD values were determined using an H-BORD device, while training volume was measured in terms of sets and repetitions. (3) Results: Statistical analysis revealed that there were no statistically significant differences in non-dominant and dominant leg peak torque parameters in the exercise groups (F = 2.65; p = 0.097; ηp2 = 0.17; F = 1.15; p = 0.0334; ηp2 = 0.084), while the total training volume was lower in the NHE + BFR group. (4) Conclusions: As a result, it was seen that adding the BFR method to NHE did not provide additional gains. However, due to the low training volume of BFR + NHE, it may be recommended to apply BFR together with NHE to athlete groups.
Image-Guided Navigation in Spine Surgery: From Historical Developments to Future Perspectives
Authors: Wilson John Preston , Fontenot Lane , Stewart Caleb , Kumbhare Deepak , Guthikonda Bharat , Hoang Stanley
Published: April 2024

Intraoperative navigation is critical during spine surgery to ensure accurate instrumentation placement. From the early era of fluoroscopy to the current advancement in robotics, spinal navigation has continued to evolve. By understanding the variations in system protocols and their respective usage in the operating room, the surgeon can use and maximize the potential of various image guidance options more effectively. At the same time, maintaining navigation accuracy throughout the procedure is of the utmost importance, which can be confirmed intraoperatively by using an internal fiducial marker, as demonstrated herein. This technology can reduce the need for revision surgeries, minimize postoperative complications, and enhance the overall efficiency of operating rooms.
Comparison of the Efficacy of Zoledronate and Denosumab in Patients with Acute Osteoporotic Vertebral Compression Fractures: A Randomized Controlled Trial
Authors: Son Seong , Oh Michael Y. , Yoo Byung-Rhae , Park Han-Byeol
Published: April 2024

Background: The comparison of the efficacy of zoledronate and denosumab for treating osteoporosis is controversial, and few randomized controlled trials have compared these two drugs in practical patients with acute osteoporotic vertebral compression fractures (OVCFs). We conducted a randomized controlled study to compare the efficacy of zoledronate and denosumab in patients with acute OVCF, with a focus on the occurrence of new OVCF. Methods: We enrolled 206 subjects who had their first acute OVCF, without any previous history of osteoporosis medication. The patients were randomly assigned to receive either intravenous zoledronate once a year or subcutaneous denosumab twice a year. We investigated the OVCF recurrence, clinical outcome, bone mineral density (BMD), and bone turnover markers over 12 months. Results: The final cohort comprised 89 participants (mean age of 75.82 ± 9.34 years, including 74 women [83.15%]) in the zoledronate group and 86 patients (mean age of 75.53 ± 10.23 years, including 71 women [82.56%]) in the denosumab group. New OVCFs occurred in 8 patients (8.89%) in the zoledronate group and 11 patients (12.79%) in the denosumab group (odds ratio, 1.485 [95% confidence interval, 0.567–3.891], p = 0.419). No significant difference was observed in the survival analysis between the two groups (p = 0.407). The clinical outcome, including the visual analog scale score for pain and simple radiographic findings, did not differ between the two groups. The changes in BMD and bone turnover markers were also not significantly different between the two groups. Additionally, drug-related adverse events did not differ between the groups in terms of safety. Conclusions: The efficacy of zoledronate was comparable to that of denosumab in terms of the occurrence of new OVCFs, as well as of the overall clinical course in patients with their first acute OVCF. Notably, this study represents the first comparison of these two drugs in patients with acute OVCF. However, further research with large-scale and long-term follow-up is necessary.
Contrast-Induced Acute Kidney Injury in Patients with Heart Failure on Sodium–Glucose Cotransporter-2 Inhibitors Undergoing Radiocontrast Agent Invasive Procedures: A Propensity-Matched Analysis
Authors: Nardi Giulia , Marchi Enrico , Allinovi Marco , Lugli Gianmarco , Biagiotti Lucrezia , Di Muro Francesca Maria , Valenti Renato , Muraca Iacopo , Tomberli Benedetta , Ciardetti Niccolò , Alterini Brunetto , Meucci Francesco , Di Mario Carlo , Mattesini Alessio
Published: April 2024

(1) Background: This single-center retrospective study aimed to evaluate whether sodium–glucose cotransporter-2 inhibitors (SGLT2-i) therapy may have a nephroprotective effect to prevent contrast-induced acute kidney injury (CI-AKI) in patients with heart failure (HF) undergoing iodinated contrast medium (ICM) invasive procedures. (2) Methods: The population was stratified into SGLT2-i users and SGLT2-i non-users according to the chronic treatment with gliflozins. The primary endpoint was CI-AKI incidence during hospitalization. Secondary endpoints were all-cause mortality and the need for continuous renal replacement therapy (CRRT). (3) Results: In total, 86 patients on SGLT2-i and 179 patients not on SGLT2-i were enrolled. The incidence of CI-AKI in the gliflozin group was lower than in the non-user group (9.3 vs. 27.3%, p < 0.001), and these results were confirmed after propensity matching analysis. Multivariable logistic regression showed that only SGLT2-i treatment was an independent preventive factor for CI-AKI (OR: 0.41, 95% CI: 0.16–0.90, p = 0.045). The need for CRRT was reported only in five patients in the non-SGLT2-i-user group compared to zero patients in the gliflozin group (p = 0.05). (4) Conclusions: SGLT2-i therapy was associated with a lower risk of CI-AKI in patients with HF undergoing ICM invasive procedures.