bluuebunny/crossref_metadata_2025 huggingface dataset:
The comparison of Faster R-CNN and Atrous Faster R-CNN in different distance and light condition
Authors: Srijakkot K , Kanjanasurat I , Wiriyakrieng N , Benjangkaprasert C
Published: February 2020

This paper presents the comparison of Faster R-CNN and Atrous Faster R-CNN, which detection model, in the different distance and light condition. Also, the dataset for model training is COCO, and the classification model is residual network. The parameter for decision the performance of the model is Mean Average Precision (mAP). The results from an object resolution at 1024x768 of Faster R-CNN at 3 meters in the evening achieved mAP 1.000. Besides, the mAP at 5 meters and 8 meters were 0.798 and 0.760, respectively. The same resolution as previous, the results of Atrous Faster R-CNN at 3 meters in the evening presented mAP 1.000. Also, the mAP at 5 meters and 8 meters were 1.000 and 0.960, respectively. In addition, Atrous Faster R-CNN had better accuracy than Faster R-CNN with appropriate range and brightness from the period of the day for real-life usage.
Position Measurement System Based on Image Trajectory Tracking Control of Directional Conveyor
Authors: Howimanporn S , Chookaew S
Published: February 2020

This paper proposes a control trajectory tracking of direction angle conveyor using image possessing. Conveyor directional is one of the most important topics in classification products. Generally, the other systems cannot be aware of real-time detection and high precision for angle tracking of object works classification. Currently, image detecting and processing systems are widely used and becoming to applying for control field in the future trend. This work installs camera webcam for visual recognition system based on using MATLAB to determine angle position and visual processing, which can recognize the area and calculate the current position of object work, incomplete angle position, and complete angle position. In the paper focus detecting directional angle to trajectory tracking of adaptive control is also responsible for enhancing angle precision between object product and position setpoint target, closed-loop control and tracking error method are designed and implemented on the programmable logic controller (PLC) for improving the efficacy of the conveyor system. The experimental close loop control presents the capability to maintain the setpoint angle position. and the adaptive error tracing with superior response of error controller. The adaptive error tracking gains using a proportional controller. The angle position control from this proposed technique can operate on the different target setpoint and improve efficiently control the directional conveyor.
Single-phase ground fault location technology research and application based on transient recording wave
Authors: Hui YIN , Changkai SHI , Baikui LI , Shilei GUAN , Yue Liu
Published: February 2020

Fault location is an important part of distribution automation. Aiming at the problem of single-phase grounding fault detection, a fault location system based on transient recording technology is proposed. Compared with the advantages and disadvantages of the traditional single-phase ground fault detection method, the single-phase ground fault location application based on transient recording is proposed. The proposed method is not only theoretically verified in the simulation of the distribution network system, but also applied to the operation of fault location network in the true test field, which verifies the effectiveness of the proposed method.
Optimizing the distribution of transmission line tower and foundation
Authors: He-yan ZHU , Jing-bo LIU , Yi-ran LI , Ao-ran XU
Published: February 2020

In the mountainous transmission lines, in order to respond to the national and national grid requirements for environmental protection, the design institute needs to design the towers for the long and short legs and the foundation of the tower. To this end, the paper gives the design scheme of the long and short leg iron tower in Liaoning area, and analyzes the design conditions of the iron tower, the base surface measurement of the iron tower, the insulation coordination and the optimization design of the tower. The purpose of this paper is to find the mutual configuration of the long and short leg towers and the high and low foundations that can be used to optimize the transmission line in mountainous projects, and use finite element software to verify that the minimum amount of base materials is the same when the tower position is constant. The basic base surface has the least amount of shovel excavation, which can better protect the geographical environment of the area where the tower is located, and can also control the amount of basic materials to a certain extent and reduce the total investment of the project.
Research on virtual synchronous generator grid-connection based on phase-locked loop
Authors: Wang Shun , Zhu Xuemei , Fan Yijie , Shao Shuai , Lei Changyan
Published: February 2020

When a single Virtual Synchronous Generator (VSG) is connected to the grid, the output voltage amplitude and frequency are significantly different from the grid voltage, which will impact the power grid. In order to solve this problem, the paper proposes a grid-connected pre-synchronization control method. By controlling the virtual synchronous generator based on the Phase Locked Loop (PLL) pre-synchronization unit, the voltage amplitude, frequency and phase of the VSG output before the grid connection can be consistent with the grid side, reducing the instantaneous impact of the grid. At the same time of the current, the output voltage of the virtual synchronous generator is stabilized. The single-machine VSG grid-connected model was built on the MATLAB/Simulink simulation platform and the effectiveness of the method was verified.
Power failure evolution model considering voltage stability
Authors: Wang Jialin , Qun YU , Wang Qi
Published: February 2020

Voltage instability is often associated with power system blackout. In order to study the evolution mechanism of power failure and voltage instability in power failure, this paper proposes a new kind of power failure evolution model that considers voltage stability. The theory of static voltage stability analysis is introduced into the modelling. According to the simulation results of power failure in an IEEE39 system, the model proposed in this paper verifies the self-organized criticality of power network. It was also found that voltage instability lead to the increase of the frequency and scale of power failure. Furthermore, the frequency of load nodes in voltage instability accidents is calculated and the set of weak nodes is obtained.
Research on energy efficiency management method of AC/DC hybrid distribution system
Authors: Zhou Juan , Wenjing XIE , Yunfeng XIA , Feng XUE , Junni SU
Published: February 2020

With the increasingly serious problems of fossil energy crisis and environmental pollution, vigorous development of Distributed Renewable Energy has become an important means of energy structure reform and energy consumption transformation. On the distribution side, AC/DC hybrid distribution system has been widely studied for its advantages of accessing multiple types of energy, satisfying diversification energy demand, realizing multi-energy complementation and improving grid security. The energy efficiency evaluation and management of AC/DC hybrid system are of great guiding significance to the planning and design of AC/DC system. However, traditional energy efficiency management mainly reduces primary energy consumption by saving electricity. For AC/DC systems with multiple distributed energy sources, it is difficult to reflect the efficiency level of the whole system by considering a single technology or a single part of energy efficiency management. Aiming at the characteristics of AC/DC hybrid distribution system, this paper studies the energy efficiency management method of the system. Firstly, combined with energy efficiency evaluation and system economic analysis, energy efficiency evaluation index of AC/DC hybrid distribution system are formulated. Secondly, the energy efficiency management method is studied through the study of adjustable load, transferable load and different types of load overall management method. Then the method of combining power quality and energy efficiency is studied. Finally the interaction between source and load is studied to improve the energy efficiency of the system. The energy efficiency management method proposed in this paper can fully tap the potential of load adjustable and energy saving, improve the comprehensive energy utilization efficiency of the system, which provides a basis for the engineering application of AC/DC hybrid power grid.
Comparison of corrosion characteristics of conductive concrete
Authors: Xu Tian , Feng Pei , Xin Liu , Lulu Jia , Chenxing Deng , Pinghao Yang , Hongbo Cheng
Published: February 2020

Conductive concrete has great application potential in grounding and electromagnetic shielding. To verify the effect of conductive concrete on the corrosion of grounded metals, several conductive concrete with different conductive phase, such as Q235 carbon steel, galvanized steel and stainless steel, were prepared. The conductive concrete test specimens are immersed in red soil leachate, their open circuit potential, potentio-dynamic polarization and electrochemical impedance spectroscopy were measured by electrochemical method. Corrosion behaviour of different grounding metals in conductive clays with different conductive phase are compared based on the pre-mentioned three indicators. The results show that conductive concrete can reduce the corrosion of grounded mental effectively compared with ordinary concrete, galvanized steel has better corrosion resistance than Q235 carbon steel, graphite-based conductive concrete can protect the grounding metal better than stainless steel-based conductive concrete, and high conductive phase content can make a low metal corrosion.
The study of intelligent transport systems management of convoy of unmanned vehicles with a lead vehicle with the purpose of increase of efficiency of cargo transportation
Authors: Endachev D. , Kolbasov A. , Karpukhin K.
Published: February 2020

This material describes the development of an intelligent transport system for controlling the movement of a column of unmanned vehicles with a leading pilot vehicle in order to increase the efficiency and safety of freight and passenger transportation in remote regions of the North of Russia, as well as in the Arctic and Antarctic. The obtained scientific results allow us to move on to the creation of new types of freight and passenger vehicles that can move unmanned modes over long distances, but at the same time, the possibility of direct human intervention remains. The introduction of this type of vehicles will improve the efficiency of freight and passenger transportation, reduce fuel consumption by predicting the traffic situation and increase the safety of transportation on public roads. The materials describe the circuit diagram of the components of the slave and leading intelligent vehicle, give mathematical models of some control systems and describe the tests that confirmed the operability of this type of vehicle. Tests have shown the adequacy of mathematical models and the complete autonomy of driven vehicles that follow the lead.
Trans-regional power trading optimization for promoting clean energy accommodation
Authors: Xinhong Shi , Yefei  , Panjiajia  , Zhangxu  , Gaoqin Wang , Jianhu Lv
Published: February 2020

A trading decision-making method that uses electricity market means to promote clean energy accommodation is proposed. Making Ml use of load difference, peak-to-valley difference and time difference, a joint optimization model of clean energy purchasing-selling-transmission is established to promote clean energy accommodation, which considers the willingness of the recipient side, and satisfies the physical operation constraints of DC line. The model can set the power curve shape, in order to realize the lean decision of cross-regional trading power, and propose a practical accommodation plan. Based on the calculation of cross-regional power transactions between two regions in China, the validity of the model and scheme is verified.
Research on the working medium of the absorption air-conditioning system with a new regeneration method
Authors: Li YH , Cheng F , Li XW
Published: February 2020

To be environmentally friendly and save energy, absorption air-conditioning system is a good attempt. However, plenty of heat waste in the regeneration limits its performance. For further application, the capacitive deionization (CDI) regeneration method has been proposed which works between the electrode pairs of the capacitor to achieve the interconversion between water and solution. Previous work shows the theoretical COP of the new system could reach up to 6 under certain conditions. Different absorbents have been calculated and experimented in this paper to find a most suitable absorbent to approach the highest possible COP. Different concentrations also has been considered. It is found that the COP of the CDI based system can attain 2 to 3. Experimental results agree well with theoretical results of the tendency of the system performance among different absorbents and different concentrations. There is some difference of the COP because the energy recovery rate in our experiment can not approach 50% which is calculated at 50% theoretically. In practical applications, the COP could be higher with energy recovery of CDI.
Real-road energy consumption characteristics of electric passenger car in China: a case study in Shenzhen
Authors: Ai Yi , Li Jingyuan , Yu Hanzhengnan , Liu Yu
Published: February 2020

As the increasing complains of the electric passenger car owners about the lower pure electric mileage under real-road driving condition than the regulation standard test result, the annual real-road energy consumption characteristics of 20 MPV electric passenger cars in Chinese city, Shenzhen, have been investigated to analyse the effect factors causing the energy consumption difference between these two results. Results showed that the annual variations of real-road energy consumption rate of each month were agreed with the variations of air conditioning using duration. The highest and lowest energy consumption rates were obtained in July and March, which increased the energy consumption rate of whole vehicle about 24% in the maximum extent. The difference of the energy consumption between real-road and regulation standard test was attributed to the effects of air conditioning using and driving condition (driving velocity and acceleration distribution), which have been qualified in this paper.
Analysis on the trading mode suitable for nuclear power in power market
Authors: Zhang Xuefei , Xiong Yi , Xiong Chuanyu , Tang Xuejun , Li Zhiwei
Published: February 2020

The energy crisis in the 21st century has become the most imperative problem to be solved by human beings. Nuclear energy is a vital base-charge power supply, which has high security, clean function and high efficiency. With the gradual development of nuclear power, the power is participating in the direct trading of the electricity market step by step. However, the rules of direct trading in China is not relatively complete, which leads to the unreasonable decline of the economy of nuclear power units. And there are drawbacks in the adjustment of energy structure, which also has a negative impact on energy conservation and emission reduction. So the author analyzes the current national nuclear power policies and electricity price costs, and proposes the current problems faced by nuclear power in direct electricity market and provides the basic directions and ideas for solving measures to help nuclear power better participate in direct electricity trading.
Dark fermentative hydrogen production from cheese whey using hydrogen-producing bacteria isolated from Mount Pancar hot spring, West Java
Authors: Gazali F M , Praharyawan S , Susilaningsih D
Published: February 2020

One of the most promising ways to produce high hydrogen yield is through dark fermentation by using dark fermentative bacteria due to the capability of these microbial agents to convert various organic compounds, particularly sugar, into hydrogen gas. In this study, three Gram-positive hydrogen-producing bacteria with a different character of colony on agar, namely as RP 009, RP 010, and RP 011, had been successfully isolated from Mount Pancar hot spring, West Java. All these isolates were able to produce hydrogen gas in all cheese whey concentration, consisting of cheese whey 30%, 60%, and 100%. RP 011 was the most favorable hydrogen producers in this study due to its high hydrogen productivity (4,400.625 ml biogas/L medium) as well as its ability to adapt and consecutively produce hydrogen even in the very high concentration of the organic compound. The best cheese whey concentration for hydrogen production in this study was 60%, considering the efficiency and effectiveness of the organic compound conversion into hydrogen gas. Ultimately, this study presented the potential of high hydrogen productivity of indigenous hot spring bacteria isolated from Mount Pancar hot spring in which had major potential for environmentally friendly bioenergy and biomass refineries.
The physical and chemical analysis of nanoemulsion from extract rodent tuber mutant plant (Typhonium flagelliforme Lodd.)
Authors: Sianipar N F , Yuliani S , Purnamaningsih R , Assidqi K , Abbas B S
Published: February 2020

Typhonium flagelliforme Lodd. is well known as rodent tuber herbal plant has been successfully irradiated by using gamma rays irradiation technique to increase the bioactive compounds. Despite the potential of rodent tuber mutant plants, the idea came up to develop functional food formulations into nanoemulsion systems. Nanoemulsion composed of extract, glycerol, DMSO, tween 80, and water in different concentrations. The rodent tuber mutant plant extract and DMSO were formulated into nanoemulsion with a fixed concentration of 0.1% of the weight of the formula, the emulsifier was used tween 80 with a concentration of 0.1% and glycerol with a concentration of 0%, 2.5%, 5%, 7.5%, 10% of the weight of the formula while the solvent used is distilled water with a final volume 10 mL of the weight of the formula. This study aimed to determine the physical and chemical characteristics of nanoemulsion prepared at various concentrations and volume ratios of rodent tuber mutant plant extract and surfactant. The analysis of the component of the nanoemulsions was conducted using Gas Chromatography-Mass Spectrometry (GC-MS). The alkane group found in the nanoemulsion sample, such as eicosane (5.96%). It can be concluded that the best extract and surfactant concentrations are using glycerol at 0%. The droplet sizes of 0% glycerol concentration had 111.6 nm and a polydispersity index of 0.541 compared with other concentrations. Nanoemulsion at 0% of glycerol showed a low zeta potential value of -19.36 mV. The destabilization of nanoemulsion after 24 h was dominated with creaming appeared, as shown in negative zeta potential. This shows that the formulation is not stable enough for a certain period of time even though it has a nanoparticle size. The development of nanoemulsion optimalization is a prospective approach to solve the stabilization problem.
Fluorene Degrading Bacteria from Indonesian Marine Environment
Authors: Ilmiah Z , Yetti E , Dahliaty A , Yopi
Published: February 2020

Fluorene is one of the Polycyclic Aromatic Hydrocarbons (PAHs) that has toxic, mutagenic, and gives a persistent effect in the environment. One of the alternative ways that can be done to overcome environmental problems due to contamination of fluorene is biodegradation using marine bacteria. There are 25 marine bacterial isolates from Laboratory of Biocatalyst and Fermentation (LBF), Research Center for Biotechnology LIPI Cibinong, Bogor which have capability to degrade fluorene. Selection of these 25 marine bacterial isolates showed that LBF-1-0144 isolate was the good relatively in degrading fluorene. This isolate was able to grow in 1500 ppm fluorene containing medium with an initial cell OD600nm of 2.5 and growth percentage of 58.652%. Molecular identification results based on the 16S rDNA gene of the LBF-1-0144 showed that this isolate had similarity of 96% with Bacillus subtilis strain 168.
Enhancement of lipid production of Chlorella sp. 042 by mutagenesis.
Authors: Rahman D Y , Rachmayati R , Widyaningrum D N , Susilaningsih D
Published: February 2020

In this study, the UV mutagenesis was performed to enhance lipid productivity in Chlorella sp. 042 isolated from East Kalimantan. One hundred colonies were screened with a method based on Nile Red fluorescence. Growth, biomass, and lipid parameters of the selected mutants (M7 and M22) were grown in a batch system of 400 mL AF6 medium and analyzed in detail. The result showed that the lipid content of M7 and M22 were higher than wild type (30.82%), 35.15%, and 43.85%, respectively. The M22 slightly had higher carbohydrate and protein content than wild type and M7. The overall result indicates that the treatment of UV mutagenesis to Chlorella sp. 042 can increase the total lipid production and other biomass content. The mutant could consecutively be used as the lipid feedstock for algal oil production.
Evaluation of Addition The Activated Charcoals and pH Adjustment in The Treatment of Lignocellulosic Hydrolisates for Xylitol Production
Authors: Thontowi A , Mayangsari W , Kholida L N , Kanti A , Wardani A K , Hermiati E
Published: February 2020

D-Xylitol is a five-carbon sugar alcohol, which can be obtaines from xylose of agriculutural wastes with microbial fermentation. The inhibitory chemical complexes (ICC) were founded after biomass pretreatment step. The ICC in acid hydrolysate can inhibit microbial metabolism in the medium. Several methods for detoxification; adsorption by activated charcoal, by ion exchange resin or by using enzymes. This study aims to evaluate the effect of using charcoal in xylitol fermentation by using lignocellulosic hydrolisate. In this work, detoxification process by added 1% activated charcoal to reduce the inhibitors in corn cobs hydrolysate and sugarcane trash hydrolysate. The biomass hydrolysate was fermented by Meyerozyma caribbica Y67 to produce xylitol. It is expected that detoxification can reduce concentration of inhibitors, so that microbial growth is not inhibited and more product. The results showed that M. caribbica Y67 had not significant different between pure hydrolysate and detoxified hydrolysate with activated charcoal. We suggested that M. caribbica Y67 tolerance to ICC and potential for xylitol fermentation from lignocellulosic biomasses.
The improvement of glucoamylase production by UV irradiated strains of Aspergillus awamori KT-11
Authors: Nuryana I , Perwitasari U , Mawey J , Ngangi J , Moko E , Melliawati R , Yopi
Published: February 2020

In many food industries, glucoamylase is extensively applied because of its ability to convert oligosaccharides into glucose. Recently, mutagenic treatments of Aspergillus awamori have been conducted to improve and enhance glucoamylase production. This research was focused on the study of glucoamylase production by strains of A. awamori after being exposed to ultra-violet (UV) irradiation. The parental strain was cultivated on the PDA plates and treated by exposing under UV lamp from 0 to 90 minutes with the time intervals of 30 min. Solid state fermentation was carried out to produce glucoamylase using cassava peel as a substrate. As compared to all strains, the mutant strain of A. awamori after being treated by 30 min of UV exposure time gave the highest activity of glucoamylase at 5.9 U/ml. The activity was two fold higher than the parental strain. The same mutant strain also reached its maximum value of total protein content at 97.31 µg/ml and 23% higher than parental strain. Glucoamylase production can be achieved and improved by the strain of A. awamori after UV mutagenic treatment.
Antimicrobial activity of ethanol fraction from cyanobacteria Chroococcus turgidus
Authors: Hidhayati N , Agustini N W S , Widyagustina D
Published: February 2020

Chroococcus turgidus belongs to cyanobacteria, a photosynthetic prokaryote, which produces many valuable bioactive compounds and shows interesting biological activities. In this study, we investigated the antimicrobial activity of the ethanol fraction from C. turgidus and identified compounds that responsible for the activity. C. turgidus biomass was extracted using the reflux method and then fractionated by column chromatography. The antimicrobial activity was evaluated against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Candida albicans using agar disc diffusion method. The result showed significant activity against all tested microbes. The largest zone of inhibition was 18.1 ±0.14 mm, achieved by the fraction against S. aureus. Characterization using Gas Chromatography-Mass Spectrometry revealed the volatile organic compounds with 1, 2-Benzenedicarboxylic acid, bis(2-ethylhexyl)ester as the dominant compound. This study revealed that the bioactive compounds produced by C. turgidus could be a potential source of natural antimicrobials.
Polyphenols Content of Indonesian Tea Clones on Optimum Manuring Condition
Authors: Rezamela E , H Syahrian K , Wulansari R , Prawira-Atmaja M I , Agustian E , Adilina I B
Published: February 2020

Tea (Camellia sinensis (L.) Kuntze) has been known as a functional drink containing bioactive polyphenols with health benefits effect. Polyphenols in tea are not only influenced by climate but also by clone types and cultivation techniques. Recently, technological developments in tea commodities are not only focused on the quantity of production but also the content of polyphenols. Previous studies reported that the application of Nitrogen (N), Phosphorus (P), and Potassium (K) fertilizers played a role in increasing the polyphenol content in tea. Research Institute for Tea and Cinchona (RITC) has released GMB Series clones that have high productivity compared to other clones. The objectives of this study were to determine the content of tea polyphenols of various GMB series clones on optimum manuring conditions. This research was carried out in 2018 in the high elevation areas (1,350 m masl) and medium elevation (800 m masl). At the high elevation area at RITC Gambung Experimental Plantation, showed that on optimum manuring condition had an effect on the increase in polyphenol content in most GMB clones (14.83 - 15.43% dw) compared to control (10.56% dw) and GMB 1, GMB 4, GMB 7 and 9 clines showed significantly different effect on the content of total polyphenols. Tests which conducted on the medium elevation area at Cianjur, West Java, showed that under optimum manuring condition, the range of total above 15% with the highest polyphenols were obtained in GMB 1 clone at the dosage application of NPKMg (300:30:249:50) kg ha−1year−1
The Main Parameter Analysis in Developing Low Pressure Biogas Standards
Authors: Benny Louhenapessy Bendjamin , Wibowo Ari , Krishna Murti Bayu
Published: February 2020

The Indonesian is one of the countries consuming fossil energy as a petroleum fuels which are quite high with the average needs increasing by 7% per year. The Indonesian Government has a commitment to address the issue of based energy crisis through one of the ways to find alternative energy sources that have the potential to generate energy that significantly to support the government program. The use of biogas is a part of renewable energy which is one of the references for government programs in order to increase energy access for the community through the utilization of New and Renewable Energy especially bioenergy. Biogas is a gas produced by anaerobic activities from organic materials including waste from human, animals, domestic and biodegradable waste or any organic waste that is biodegradable under anaerobic conditions. Biogas is a very interesting gas fuel to be developed because it can be updated and can be made by yourself with uncomplicated simple technology. The purpose of this study was to analyze the main parameters in the low pressure biogas quality requirements. This study uses descriptive qualitative analysis using the soft ware Framework for Analysis, Comparison, and Testing of Standards. The stages of this study include determining the basic principles, formulation and proof of standard concepts, stakeholder analysis, technical analysis and comparison of standards. The research locus includes producers, consumers, technical committees and conformity assessment agencies of biogas. Primary data collection and surveys include 10 provincial cities in Indonesia. This research recommends several parameters that can be used as a basis in developing national and international standards include low pressure factors; gas content including methane; hydrogen sulfide; carbon dioxide and oxygen.
The grow-out of abalone (Haliotis squamata) at different shelter shape on growth and survival and its marine environmental influences at Lembongan Bay coastal waters
Authors: Ardi I , Setiadi E , Rasidi  , Pranowo W S
Published: February 2020

Abalone has an economic value in the world. The larval rearing technique of abalone has produced successfully at Gondol Research and Development Institute for Mariculture, Bali, Indonesia. However, the grow-out of abalone has not well documented yet. The purpose of this experiment is to examine the shelter shape in relation to growth and survival for abalone (Haliotis squamata) grow-out culture in the tidal area. The shelter made from PVC piping. The shape of shelter as a treatment of this experiment was A) round; B) square and; C) without shelter. The data of growth and survival rate were analyzed using analysis of variance. The result showed that the shelter for the grow-out of abalone was significantly affected growth in terms of shell length and wet weight (P<0.05) while the survival did not influence (P>0.05). It seems that the shelter was correlated with the feeding behavior of abalone had resulted in different for their growing. The water quality such as salinity, dissolved oxygen, water temperature, and water current was within the optimal range for abalone. We found that round shelter shape is the best for grow-out abalone at the tidal area.
Determination of minimum inhibitory and minimum bactericidal concentration of ketapang (Terminatia catappa) leaves extract against Vibrio harveyi
Authors: Kharisma A , Tjahjaningsih W , Sigit Setiawati
Published: February 2020

Vibrio harveyiis a bacteria that causes Vibriosis in shrimp and can cause mass death. The use of antibiotics and chemicals continuously can harm the environment and humans as consumers. Ketapang leaves (Terminalia catappa) are natural ingredients that can be an alternative to antibiotics and chemicals used to suppressV. harveyibacteria. The purpose of this study was to determine the antibacterial activity of ketapang leaf extract againstV. harveyiin vitro using the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods. The experimental study used a completely randomized design (CRD) with 14 treatments and three replications. The results showed that ketapang leaf extract was able to inhibit and killV. harveyibacteria in vitro. The minimum concentration of ketapang leaf extract which can inhibitV. harveyibacteria was 1.56% and the one who was able to kill was 3.12%. This proves that ketapang leaves can be used as an antibacterial againstV. harveyi
Use of hydrogen peroxide to improve potential redox land preparation of land towards increasing production of traditional shrimp vanname (Litopeaneus vanname) in Wringin Putih, Muncar, Banyuwangi
Authors: Nindarwi D D , Sari L A , Wulansari P D , Samara S H , Santanumurti M B
Published: February 2020

Land preparation in a traditional shrimp pond is one of the most important parts to do. Soil Redox Potential is an indispensable parameter to see the readiness of soil land or the level of fertility of cultivated land in carrying out activities. It is toxic to shrimp such as sulfide compounds (H2S), nitrites and ammonia. This research is descriptive in nature, by observing 5 traditional aquaculture ponds measuring 3500 m2, with shrimp densities of 100 individuals/m2. The use of Hydrogen Peroxide is pool 1 (0.5 ppm/m2), pool 2 (1 ppm/m2), pool 3 (1.5 ppm/m2), pool 4 (2 ppm/m2) and pool 5 as a control (0 ppm/m2). The basis for determining the use of Hydrogen Peroxide doses is from a minimum reference dose. The best results in pool 3 with a potential reduction value of 331 m/v, wherein one period the value of NH4 ranged from 0-2 ppm with ADG (Average Daily Growth) every week an average of 0.3 grams.
Histopathological analysis of Pangasius sp. infected by Edwardsiella tarda causes Edwardsiellosis disease
Authors: A’yunin Q , Budianto  , Andayani S , Yuwanita R
Published: February 2020

Pangasius spare included in the group of catfish that have high economic value. Catfish are susceptible to infection with Edwardsiellosis caused byEdwardsiella sp. Edwardsiellosis is one of disease which causes declining of catfish production in Indonesia. Histopathological test can be one way to diagnose a disease infection by observing cell or tissue changes. The method used in this study is descriptive and sampling method by exsitu, by taking samples from the field and observing in the laboratory. The results showed tissue changes in the organs ofPangasius sp. due to bacterial infection which leads to damage hyperplasia, lamella fusion, and necrosis.
The use of bromelain enzyme on artificial hatching media as an effort to hatch Nile tilapia (Oreochromis niloticus) eggs outside the mother’s mouth
Authors: Fariedah F , Widodo M S , Nuswantoro S , Sholikhin
Published: February 2020

Nile tilapia is a type of mouthbreeder fish which is a fish that incubates its eggs in the mouth. The parent fish that do spawning must incubate eggs for up to 12 days and fast until the eggs in their mouths hatch, it can cause nutrient deficient and a long recovery period that will affect the subsequent production. The conditions on artificial tilapia eggs hatching media are made to resemble the conditions in the mouth of tilapia, one of which is the presence of the protease enzyme. One of the protease enzymes that can be used is the bromelain enzyme found in pineapple. This study used the experimental method and used a completely randomized design (CRD) trial with three replications and five treatments. Bromelain enzymes used were A (0, 09 µmol), B (0.105 µmol), C (0, 12 µmol), D (0.135 µmol) and E (0.15 µmol). The parameters observed were hatchability and survival rate. The results suggested that the bromelain enzyme can be used to do tilapia egg hatching outside the mother’s mouth with the highest hatching rate was 74.44%. But it does not affect the survival rate of tilapia larvae with a survival rate was 82.5%.
The variances of hematology of gurami (Osphronemus gouramy) which is vaccinated and challenged by Aeromonas hydrophila
Authors: Alfaniah D , Kusdarwati R , Rozi  , Handijatno D
Published: February 2020

Freshwater fish that became the leading commodity of gourami. Fish cultivation is often experienced constraints of infection from motile aeromonas septicaemia (MAS), also known as red spotting disease caused by bacteria Aeromonas hydrophila. A vaccine is an effective preventive measure because in this way immune can be obtained only once or twice vaccine administration until the fish is harvested. This study aims to determine the haematological picture of gourame (Osphronemus gouramy) that vaccinated and challenged bacteria Aeromonas hydrophila. This research is an experimental research using completely randomized design which consists of 5 treatments and 4 replications. The treatment of this study was the difference of vaccine dose ie A (control +) fish injected vaccine without any challenge test, B (control -) fish injected NaCl Physiological and tested challenge, C vaccine dose 104 CFU/mL, D vaccine dose 105 CFU/mL, and E vaccine dose of 106 CFU/mL. The results of this study showed vaccinations and tests of bacteria Aeromonas hydrophila in gouramy can give effect to total erythrocytes, hemoglobin, leukocyte total, and leukocyte differential. The 106 cell/mm3 (E) vaccine dose provides the best (responsive immune response) effectiveness compared to the other two doses.
Addition of lemuru fish oil in feed on the gonadal maturity level of female silver barb, Rasbora argyrotaenia
Authors: Agustin L , Sulmartiwi L , Mubarak A S
Published: February 2020

The development of aquaculture technology to produce silver barb is constrained by the low fecundity and long space of reproduction periodicity. As a consequence, feed manipulation is necessary to improve fecundity and gonadal maturity. Fecundity and gonadal maturity could be developed through additional fat from lemuru fish oil. Lemuru fish oil contains high Omega-3 that frequently used in the vitellogenesis process. The objectives of this study were adding lemuru fish oil in feed to increase the gonadal maturity level of the female silver barb. This research applied a completely randomized design (CRD) method which consist of five treatments (0%,2%,4%,6%,8%) in adding the different concentration of lemuru fish oil in feed and four replications for each. The feeding was done three times a day, at 4% biomass in 42 days of treatment. The parameters observed in this study were the gonadal maturity level in morphology as well as histology, gonad somatic index (GSI), gonad index (GI) of the female silver barb. The results of this study indicated that the addition of lemuru fish oil at 4% concentration in feed was able to accelerate 60% of the population of female silver barb gonadal maturity in the level of III and IV.
Chlorophyll and carotenoids analysis spectrophotometer using method on microalgae
Authors: Rinawati M , Sari L A , Pursetyo K T
Published: February 2020

Microalgae is a very potential to be developed biota Because of its abundant amount on earth. Research on microalgae and its application as pigment analysis, provides important information about chemical compounds such as carotenoids and chlorophyll roomates that can be developed into drugs. Microalgae such as Chlorella vulgaris, Nannochloropsis sp., Porphyridium cruentum and Spirulina platensis has known had carotenoid pigments and chlorophyll content, but the optimization of the production of pigment in microalgae life phase has not been known in detail. Chlorophyll content in microalgae in the logarithmic phase of Chlorella vulgaris 200-1500 mg/L, Nannochloropsis sp. 100-500 g/L, Porphyridium cruentum 500-800 g/L and Spirulina platensis 1000-3500 mg/L and for stationary phases microalgae Chlorella vulgaris 100-1000 mg/L, Nannochloropsis sp. 200-500 g/L, Porphyridium cruentum 900-2000 mg/L and Spirulina platensis 2000-6000 mg/L. While the carotenoid content of microalgae in the logarithmic phase of Chlorella vulgaris 10-40 g/L, Nannochloropsis sp. 10-60 g/L, Porphyridium cruentum 10-60 ug/L and Spirulina platensis 20-40 ug/L and for stationary phases microalgae Chlorella vulgaris 10-50 g/L, Nannochloropsis sp. 10-70 g/L, Porphyridium cruentum 70-130 ug/L and Spirulina platensis 20-1100 mg/L.
The effect of catfish and chicken cultivation waste to Daphnia sp. culture
Authors: Holy N H , Sari L A
Published: February 2020

Many factors influence the success of a cultivation business, the availability of the land, the availability of adequate fry and feed in quality and quantity to support maximum production. The study is to know the effect of catfish and chicken cultivation of waste to Daphnia sp. culture. Which is it does not pollute the culture medium and the high nutrient content. Daphnia sp. culture activity is divided into laboratory and mass scale. Culture activity started by sterilization and preparation of culture equipment and continued with Daphnia sp. forage provision. Stocking density that used in laboratory or semi mass scale is about ten ind/ml Daphnia. Duration of culture activity of Daphnia sp. was ten days and use water from catfish pond as media resulted from the highest density of Daphnia sp. with 5355 ind/liter produced during a period in laboratory scale. Daphnia culture in laboratory scale with molasses and plankton as a nutrient booster resulted in 1768.5 ind/liter, and it is lower than the Daphnia culture that uses water from catfish pond as media. The result in the spring-mass scale of Daphnia sp. culture was 451.400 ind/liter in 10 days period.
Degree of nematode endoparasite infection in asian swamp eel (Monopterus albus) from Banyuwangi regency
Authors: Bakti D I , Mahasri G , Ulkhaq M F , Kismiyati  , Budi D S , Suciyono
Published: February 2020

Banyuwangi district has widely rice field with big potential in agriculture and fisheries such as Asian swamp eel (Monopterus albus). Endoparasites infection in Asian swamp eel happened by consumption of natural feed that infected with endoparasites larvae. This research aims to identify and determine the prevalence and intensity of nematode endoparasites that infected to Asian swamp eel (M. albus). Three hundred asian swamp eel (30-110 cm length and 29,8-60 g weight) were collected from five traditional market in Banyuwangi district (Banyuwangi City, Srono, Cluring, Rogojampi and Genteng). The result showed that Asian swamp eel from Banyuwangi district was infected by Eustrongylides ignotus with the highest prevalence and intensity, 16,33% and 1,92 respectively than another endoparasite (Procamallanus and Pingus sinensis). This result expected to base for further study to determine the prevalence and intensity of endoparasite in a different season for mapping the spread of endoparasite in Asian swamp eel at Banyuwangi district.
Inventory of ectoparasites in pacific white shrimp (Litopenaeus vannamei) that cultivated with high density
Authors: Pamenang G D , Sulmartiwi L , Mahasri G , Rahayu N D , Angwarmas B
Published: February 2020

Ectoparasites can attack on pacific white shrimp (Litopenaeus vannamei) which are kept in high density concrete ponds. The purpose of this study was to determine the type, prevalence, infestation and intensity degree of ectoparasites pacific white shrimp Postlarva (PL11 – PL15) stage and Postlarva (PL20 - PL30) stage in Bangil ponds that are cultivated with high density. The method used in this research is a survey method with random sampling. Data were analyzed using T test. The results of this study indicate that there are three genus of ectoparasites both in the pacific white shrimp PL11 - PL15 stage and PL20 - PL30 stage, namely Zoothamnium sp., Vorticella sp. and Epistylis sp. The prevalence of ectoparasites in pacific white shrimp is in the PL20 - PL30 stage at 100% while the prevalence of ectoparasites in the PL11 - PL15 stage is 75%. The level of ectoparasite intensity that attacks the pacific white shrimp (PL11 - PL15) includes a moderate category of 21.8 individuals/head, while the level of intensity of ectoparasites that attacks the pacific white shrimp (PL20 - PL30) includes a very severe category that is 814.05 individuals/head.
Length weight relationships and condition factor of sweet river prawn, Macrobrachium esculentum (Thalwitss, 1891) in the downstream Rongkong watershed
Authors: Jurniati  , Arfiati D , Maizar A , Kurniawan A
Published: February 2020

Swett River Prawn, Macrobrachium esculentum (Thalwitz,1891) is one of the freshwater prawns’ species that have important economic value and is the least concern species in the IUCN red list. The purpose of this study was to determine the length-weight relationship and condition factor of M. esculentum in the downstream watershed Rongkong. Prawns’ sampling was conducted from September 2018 to February 2019 from the artisanal fishery in the downstream watershed Rongkong. using method simple of Random Sampling. M. ecsulentum obtained as many as 244 Prawns’ consisting of 147 male and 97 female. The results showed that the long weight relationship for males was W = 0.712L0.2865 and females W = 11.137 L3.0537. b value for males is smaller than 3, growth is allometric negative while females are positive allometric (b>3). Average condition factor values for male and female shrimp are 275.28 and 1.88, respectively. The average relative weight (Wr) values for males and females are 100.2 and 101.9, respectively, which shows that the waters are still in good condition.
Biology, Ecology and Aquaculture potential of Osteochilus spilurus (Bleeker 1851) in East Belitung, Indonesia
Authors: Kurniawan A , Hariati A M , Kurniawan A , Kartika  , Rizkika N , Wiadnya D G R
Published: February 2020

Osteochilus spilurus is one of the freshwater fish species in the Cyprinidae family which is found in the ancient river region of Sunda shelf which empties toward the North Natuna Sea. This article explores the biological, ecological, and potential characteristics of fish called Cempedik (local name in Belitung Island) as an aquaculture species. Research results and literature showed about feeding habits, reproduction, habitat, and aquaculture potential. It is abundant and well suited for culture in an established market insure, especially Belitung Island, but reproductive biology is not well understood. It can be preserved in an artificial environment, and it is possible to become aquaculture species as well as other osteochilus genera.
Antiproliferation effects of Glycine max Linn ethanolic extract on induced mammary gland carcinoma in albino rats
Authors: Triana N M , Wilujeng E , Putri M W H , Yuda D M P , Hardiono A L , Purnama M T E , Fikri F
Published: February 2020

This study aimed to determine the antiproliferation effect of Glycine max Linn ethanolic extract on Sprague Dawley albino rats induced by 7,12-dimethylbenz(á)anthracene (DMBA) on the mammary gland. This study used 20 albino rats then divided into five treatment groups, i.e., positive control (C+) induced DMBA; negative control (C-) none treatment; Treatment groups (T1; T2; T3) induced DMBA then followed extract dose (5; 10; 20 mg/day). DMBA induction was given every two days for 20 days subcutaneously. Administration of Glycine max Linn extract was given peroral for 14 days. It can be concluded that Glycine max Linn ethanolic extract inhibits the proliferation cell in mammary gland carcinoma in albino rats revealed on aspect hyperchromatization, tubular formation, and shape of the nuclei.
Effect of different carbon doses of tapioca (Manihot esculenta) flour on vegetative cells and spore production of Bacillus megaterium
Authors: Mahariawan I M D , Ariffin N B , Kusuma W E , Yuniarti A , Beltran M A G , Hariati A M
Published: February 2020

Bacillus megaterium is a spore forming bacteria that is mostly used as a probiotic in aquaculture. Spore formation for probiotic production using carbon source is costly. This research evaluated the effect of different carbon doses of tapioca (Manihot esculenta) flour on vegetative cells, spore production, sporulation efficiency and spore germination of B. megaterium. Experiments were carried out in Aquaculture Laboratory, University of Brawijaya. In flasks, 50 ml of growth media were used and were inoculated with 1% of B. megaterium (2.6 x 108 cells/ml). The cultures were mixed (120 rpm) and incubated at 37 °C for 120 hours with 4 different treatments of carbon doses (5, 10, 15 and 20 g/L, respectively). The results showed that the highest vegetative cells (8.4 x 107 cell/ml) and spore production (4.1 x 107 spore/ml) of B. megaterium were found at 15 g/L carbon dose. However, it was not followed by high sporulation efficiency (only 49.01%). The high spore germination was observed in more than 5 g/L carbon dose. Thus, 5-15 g/L carbon doses of tapioca flour could positively enhance vegetative cells, spore production, sporulation efficiency and spore germination of B. megaterium and could be used as a potential source of probiotics in aquaculture.