bluuebunny/crossref_metadata_2025 huggingface dataset:
Desafios e possibilidades do novo Ensino Médio a partir da perspectiva dos professores de uma Escola da Rede Pública
Authors: Melo Lívia Ferreira de , Rocha Josefa Eleusa da
Published: June 2023

A Lei de nº 13.415/2017 instituiu o Novo Ensino Médio e estabeleceu determinadas formulações, como por exemplo, o aumento da carga horária e as alterações curriculares, trazendo novos desafios e possibilidades para o meio escolar. Com isso, o objetivo do estudo foi verificar os desafios que o Novo Ensino Médio está trazendo para os professores, além de identificar quais os conteúdos são mais utilizados para contextualizar com o cotidiano dos alunos. A pesquisa tem caráter experimental, classificada como qualitativa quanto a abordagem. A coleta de dados ocorreu através de uma entrevista com 10 professores, nos dias 13 a 15 de março de 2023, onde foi possível realizar um convite verbal para participar do projeto e em seguida assinar o Termo de Consentimento Livre e Esclarecido. Após a coleta de dados, os resultados foram tabulados no programa Excel 2013 e transformados em gráficos e tabelas. A partir da entrevista foi possível identificar que os professores tem idade bastante variada, indo desde 20 a 29 anos até 50 a 59 anos, também foi visto que os recursos didáticos mais utilizados foi o livro didático, revistas, cartazes, desenhos e Datashow, já os conteúdos mais utilizados para contextualizar com a rotina dos alunos foi a genética, a anatomia, a fisiologia e a ecologia. Assim, foi possível identificar as dificuldades que os professores estão sentindo devido as transformações que o Ensino Médio vem passando, e que a pouca quantidade de formação continuada oferecida acaba deixando os professores inseguros frente à nova era do ensino médio.
Comparação de acurácia entre testes sorológicos e moleculares para detecção da infecção por SARS-CoV-2: revisão sistemática
Authors: Oliveira Júnior Márcio Moçambite de , Souza Carlos de Miranda , Brandão Suziany , Almeida Anne Cristine Gomes de
Published: June 2023

Introdução: No início do surto causado pelo SARS-CoV-2 em 2019, foi necessário a criação de métodos de diagnósticos para essa nova cepa da família do coronavírus. O diagnóstico muita das vezes era difícil, e era debatido qual o melhor método para detectar da infecção: O teste sorológico ou molecular. Objetivo: Comparar a sensibilidade, especificidade e usabilidade dos testes sorológicos e moleculares na detecção do SARS-CoV-2. Metodologia: Essa pesquisa é uma revisão sistemática. Resultados: Foram selecionados 8 artigos científicos e 2 bulas de teste diagnóstico para composição desta revisão sistemática. O teste sorológico em relação a usabilidade não apresenta dificuldades na logística, seu custo-benefício de uma forma geral apresentasse mais acessível e são exigidos apenas espaços físicos de baixa complexidade para sua aplicação. No que diz respeito a Sensibilidade do teste sorológico, a média dos resultados identificados foram 85,7% para o IgM e 92,8% para o IgG. A média de Especificidade teve alcance de 100% para ambos. Em contrapartida, o teste molecular, no que diz respeito a usabilidade, apresenta alta dificuldade na sua logística, seu custo-benefício é elevado, exige espaço físico de alta complexidade com equipamentos restritos a este local (laboratório) e um profissional técnico de alta qualificação. De modo geral as porcentagens identificadas de Sensibilidade se apresentaram em 95% e 100% de Especificidade na detecção do vírus SARS-CoV-2. Conclusão: O teste molecular, mostrou-se mais eficaz quando comparado ao teste sorológico na detecção da infecção por SARS-CoV-2. Em contrapartida, no que diz respeito a usabilidade o teste sorológico apresentou menos obstáculos em relação ao teste molecular.
ANKSİYETE VE UYKU BOZUKLUKLARINDA KULLANILAN TIBBİ BİTKİLER
Authors: BAYIR Ecenur , ELGİN CEBE Gözde
Published: June 2023

Amaç: Anksiyete ve uyku bozuklukları ülkemizde ve dünyada son yıllarda giderek yaygınlaşan psikiyatrik hastalıklardandır. Anksiyete ve uyku bozukluğu olan bireyler hastalıklarının esas tedavisi olan farmakoterapi ve psikoterapiye ek olarak farklı tamamlayıcı tedavi arayışlarına yönelmektedir. Bu tamamlayıcı tedavi yöntemleri arasında en sık başvurulan kaynakların tıbbi bitkiler olduğu kaçınılmaz bir gerçektir. Fakat bu ürünlerin “yan etkisi daha az/daha zararsız/daha etkili” olabileceği düşüncesi gözden geçirilmesi gereken önemli bir konudur. Bu derlemede anksiyete ve uyku bozukluklarında kullanılan tıbbi bitkilerin PubMed, Science Direct, Google Akademik, BioMed Central, TÜBİTAK Ulakbim veri tabanlarında detaylı bir literatür taraması ile belirlenmesi, en sık kullanılanlarının ise hastalıklar üzerindeki potansiyel etki mekanizmalarının incelenmesi ve olumlu/olumsuz etkilerinin ortaya konması amaçlanmıştır.
Sonuç ve Tartışma: Literatürdeki çalışmalar değerlendirildiğinde; anksiyete ve uyku bozukluklarında kullanılan tıbbi bitkilerin fazlalığına rağmen birçoğunun potansiyel etki mekanizması ve olumlu/olumsuz etkileri tam olarak ortaya konmamıştır. Bu bitkilerin yanlış ve bilinçsizce kullanımı hastalığın seyrinin kötüleşmesine yol açabileceğinden anksiyete ve uyku bozukluklarında kullanılan tıbbi bitkilerin incelendiği daha fazla araştırmaya ihtiyaç duyulmaktadır.
An empirical study to explore modular courses for the development of intelligence retail to the elimination of differences between theory and application in Taiwan
Authors:   Department of Marketing and Logistics, China University of Technology, Taiwan., Chiang Kanglin
Published: June 2023

This paper focused on modulation courses for intelligent retail services to alleviate the discrepancy between learned theory and application. Using the IPO (Bushnell, 1990) method and the ADDIE (Hodell, 2012) method serve as the theoretical basis for the analysis of questionnaires collected before and after the course with a Paired-sample t-test and Multiple Regression Analysis. How the reduction of theory-application discrepancy and guiding students to seek further professional knowledge are the aim of modulated courses. This research found that students must have the drive and thirst for knowledge to eliminate differences between theory and application. Keywords: Learning strategy, digital development course, intelligence retail, learning to use, pragmatic.
Reflection and peer assessment to promote self-directed learning in higher education
Authors:   Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China., Dutta Shanta , He Mingjing ,   Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China., C.W. Tsang Daniel ,   Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
Published: June 2023

Reflection and peer assessment as pedagogical approaches were implemented through a group project in this study aimed to transform students into reflective and self-directed learners thereby enhancing their competence and learning achievements. To support students’ learning progress and evaluate their learning outcomes, formative and summative assessments were conducted halfway through and at the end of the project, respectively, under the guidance of the course instructor. Self-reflection as a systematic reviewing process during the formative assessment enabled students to critically evaluate their own learning behavior and identify intrinsic learning needs, while team members were encouraged to provide constructive feedback to peers which could play a crucial role in improving their overall learning experiences. In contrast, the summative assessment exercise allowed students to grade their peers within each group considering pre-assigned criteria as guided by the course instructor. The summative assessment indicated contrasting performance among the two participating groups, which could be attributed to their differences in active engagement, peer interactions, and collaborative learning during the project that eventually influenced the group's performance. Though both teams were comparable in terms of competence, effective collaboration and organization among group members supplemented the learning process and turn their performance distinct. However, in some cases, the realization of learning needs was not transformed into necessary actions, which was eventually reflected in the group performance. Overall, this study demonstrated that reflection and peer assessment practice are conducive to the development of critical thinking and self-directed learning among students, which can augment their learning experiences at the university level. Keywords: Self-directed learning, active learning, reflective practices, formative assessment, contructivism, transformative learning theory.
THE INTERACTION OF TUNGSTEN (VI) OXIDE AND CALCIUM TUNGSTATE IN THE CaCl2–NaCl EUTECTIC MELT
Authors: Medvezhynska Olha , Omel'chuk Anatoliy , Shvaika Irine , Shvayka Igor , Proskurka Lubov
Published: June 2023

The interaction of tungsten (VI) oxide and calcium tungstate with a molten eutectic mixture of calcium-sodium chlorides in the temperature range from 600 to 800 °С was investigated by the methods of isothermal saturation, mass spectrometry with inductively coupled plasma (ICP–MS) and X-ray phase analysis. It was noted that the solubility of both tungsten trioxide and calcium tungstate depends to a large extent on temperature. Thus, in the temperature range from 600 to 700 °C, the equilibrium concentration of tungsten increases by an average of 1.7 times, and in the range from 700 to 800 °C, its values increase by 3.9 times.
Based on the results obtained by inductively coupled plasma mass spectrometry, it was found that the equilibrium content of tungsten (in the calculation of pure metal) in the molten eutectic mixture of sodium and calcium chlorides both in contact with tungsten trioxide and in contact with calcium tungstate at different temperatures within the error of definition has approximately the same values. The obtained results indicate in favor of the fact that the dissolution of tungsten trioxide in the molten mixture of sodium and calcium chlorides proceeds through the stage of formation of calcium tungstate. It is possible that sodium tungstate may be formed in this case, but no direct evidence of this has been found. At temperatures higher than 750 °C, almost all of the loaded tungsten trioxide turns into calcium tungstate. This gives grounds for asserting that in the molten salt phase tungsten is mainly in the form of calcium tungstate.
The obtained results give reason to consi­der that the interaction of the mentioned oxy­gen-containing compounds of tungsten produces homogeneous salt phases of the ternary system CaCl2–NaCl–CaWO4, the content of which calcium tungstate in the temperature range of 700÷800 °С is satisfactorily described by the dependence ln S = 8.331 – 14057/T.
The role of individual neuronutrients in optimizing nutritional therapy for neurological patients
Authors: Orlova S. V. , Nikitina E. A. , Balashova N. V. , Gribakin S. G. , Prokopenko E. V. , Vodolazkaya A.  N. , Pigareva Yu. A.
Published: June 2023

Neurodietology is a multidisciplinary science based on knowledge of neuroscience and dietology and aiming to optimize the treatment of neuropsychiatric diseases through qualitative and quantitative modulation of the composition of diets, alimentary prevention of diseases of the nervous system, when the latter is possible, as well as optimization of psychomotor and intellectual functions. It should be noted that neurodietology and approaches to the choice of therapeutic nutrition every year occupy an increasingly strong position in world clinical medicine. The variability of nutritional approaches to the practical treatment of neurological pathology in patients reflects the versatility of modern neurodietology. Therefore, it is no coincidence that childhood neurodietology has been widely used in Russia, which cannot be said about nutritional approaches in the treatment of adult patients of this profile. In addition, violations of the nutritional status and the lack of its timely correction reduce the effectiveness of rehabilitation measures – the most important link in the complex treatment of neurological patients, prevention of disability and increasing social adaptation. If it is impossible to provide an adequate level of intake of nutrients and energy in the standard way, it is necessary to prescribe nutritional support in a timely manner using specialized food products, including biologically active food supplements.
The problem of magnesium deficiency and metabolic syndrome
Authors: Nikitina E. A. , Orlova S. V. , Balashova N. V. , Pigareva Yu.  A.
Published: June 2023

The epidemic of obesity, insulin resistance and diabetes mellitus currently observed in developed countries requires new approaches to the prevention and treatment. Besides the hypercaloric diet micronutrient deficiency is involved in the development of metabolic disorders. Recent diet changes due to the agrarian and food technological revolutions has led to the emergence of new micronutrient deficiencies, primarily magnesium. While its hypotonic action is well studied, magnesium’s positive effects on metabolism, anti-inflammatory and antioxidant effects are currently the focus of researchers’ attention. The magnesium preparations reduce insulin resistance, improves anthropometric and laboratory markers of the metabolic syndrome. Detection of magnesium deficiency, including subclinical one, and correction of magnesium status are important tasks for the prevention and complex therapy of the metabolic syndrome. Given the higher bioavailability and lower incidence of side effects, organic magnesium salts are preferable.
Antiviral properties of breast milk: a new area of research
Authors: Gribakin S. G. , Orlova S. V. , Podoprigora I. V.
Published: June 2023

Human milk uniquely combines nutritional, immunological, enzymatic and hormonal properties. The properties of secretory IgA (sIgA) have been studied for more than half a century, the role of oligosaccharides has been studied for more than 30 years, and the importance of breast milk stem cells has been discussed for more than 10 years. Wide interest, including in connection with SARS-CoV-2-, HIV-, rota-, norо-, herpes- and other viral infections, was caused by various (more than 10 types) protective factors with antiviral activity present in human milk. This review discusses the properties and significance of immunoglobulins, lactoferrin, oligosaccharides, lysozyme, cytokines as antiviral factors of both scientific and practical importance.
The possibilities of using modern preventive technologies in the work of specialists of various profiles to assist the family in organizing proper nutrition of the child
Authors: Gorelova Zh. Yu. , Guryanova M. P.
Published: June 2023

Preventive technologies contribute to the formation of a healthy lifestyle, healthy nutrition, the development of healthy eating habits and nutrition culture among schoolchildren. The article presents the ways of using and the possibilities of using modern preventive technologies to increase the motivation of students to a proper lifestyle, nutrition in the family and at school, raising awareness of students about healthy nutrition. When using them, it is necessary to carry out educational work at school and in the family with parents, with children, including medical professionals, teachers. The division of functions of specialists of different profiles to assist the family in the organization of proper nutrition of the child is indicated, what actions should be taken by a social service worker: a social pedagogue, a specialist in social work, a specialist in working with the family, so that parents competently and responsibly approach the nutrition of the child in the family. Digital learning models and the correct organization of their use provide additional opportunities for the formation of new and development of previously acquired skills and principles of proper, healthy nutrition, and the development and use of additional tools increases the literacy and effectiveness of teaching children and adolescents in the field of healthy nutrition. The effectiveness of the work carried out depends on a properly organized set of preventive measures by specialists of various profiles.
Functional Ingredients for specialized Foods: Issues to be Addressed
Authors: Kodentsova V. M. , Risnik D. V. , Kryukova E. V. , Dariy S. G.
Published: June 2023

Review was carried out using the RSCI, Google Scholar, Pubmed, and ReserchGate databases. When biologically active substances (BAS) are used as functional ingredients in the composition of specialized food products (SPP) of dietary therapeutic and dietary preventive nutrition, the problems of choosing their dose arise. There are epidemiological data on the level of consumption of BAS with food, an associative relationship between the level of consumption of biologically active substances and the risk of diseases, as well as the results of assessing clinical efficacy are described. The content of BAS is regulated by regulatory documents that establish an adequate and upper permissible level of consumption as part of the SPP.

The purpose of the review is to assess the level of dietary intake of curcumin, carotenoids, β-glucans, and taurine, and to compare the doses allowed for use as part of the SPP with doses that provide a clinical effect. An analysis of literature data showed that the consumption of taurine with a normal

diet is 50–400 mg, curcumin – 10–1500 mg, β-carotene – 0.8–10 mg, lycopene – 5–10.5 mg, lutein – 1–3 mg, zeaxanthin – 0.1–0.6 mg, cereal β-glucans – 0.7–2.8 g, mushroom β-glucans – 0.9–1.8 g. With the exception of β-glucans, especially cereals, these values do not exceed the current upper allowable consumption level as part of the SPP. The results of clinical trials have shown that effective daily doses for lutein are 15 mg, taurine are 1.5–3 g, curcumin are 300–1600 mg, cereal β-glucans are 3–8 g, mushroom and yeast β-glucans are about 1 g. These data indicate the need to increase the amount of BAS as part of the SPP of dietary therapeutic nutrition to clinically justified values. At the same time, the given data indicate the expediency of differentiating the SPP, depending on the doses of BAS, into 2 categories: SPP for preventive and SPP for therapeutic action. SPP for dietary preventive nutrition is necessary to compensate the insufficient intake of essential micronutrients and achieve an adequate level of consumption of BAS, which will reduce the risk of alimentary-dependent diseases. In order to provide a clinical effect in pathological conditions, dietary therapeutic SPP should contain higher doses of biologically active substances that exceed the adequate intake level, but do not reach the upper safe intake level.
Comorbid food allergy in patients with congenital epidermolysis bullosa
Authors: Galimova A. A. , Makarova S. G. , Murashkin N. N. , Snovskaya M. A.
Published: June 2023

Introduction. Inherited epidermolysis bullosa belongs to the group of severe rare hereditary mechanobullous diseases. Often, the skin pathological process is difficult to treat, which leads to a decrease in the quality of life of such patients. The mechanism of development of transcutaneous sensitization in this category of patients is not excluded. This issue remains a very relevant area for study, given the characteristic nutritional deficiency and the difficulties that arise in the formation of the diet.

The aim: to assess the frequency of occurrence and characteristics of food sensitization in children with epidermolysis bullosa.

Materials and methods: the group included 164 children with epidermolysis bullosa (45 with rapid detection and 119 with dystrophic). For all patients, an assessment of the risk of an allergic history, determination of the total level of IgE and specific IgE of blood serum to the most significant food allergens (UniCAP system, Thermo Fisher Scientific). Results: food sensitization was detected in 34.1 % of children with epidermolysis bullosa (in 38.7 % of cases with dystrophic and in 24.4 % with a simple form of epidermolysis bullosa). Among the manifestations of food allergy in both groups, skin symptoms were more common. The most common etiological factors were products containing cow’s milk protein, eggs, and cereals. In the group of children with comorbid food allergies and epidermolysis bullosa, high and extremely high levels of total IgE were most common.

Conclusion: a high frequency of food sensitization in patients with epidermolysis bullosa, was shown, which is important not only from a scientific, but also from a practical point of view. Given the nutritional deficiency characteristic of this disease, the complexity of nutritional support, the presence of comorbid food allergies should be taken into account when recommending nutrition and selecting therapeutic products for this category of patients.
Narrativas juveniles sobre el narcotráfico en Sinaloa: ingreso, riesgos y planes a futuro
Authors: Valdez Bátiz Jairo Elí , Esparza Bernal Víctor Horacio , Burgos Dávila César Jesús
Published: June 2023

The article’s objective is to understand the youth experiences, assessment, and meaning construction of drug trafficking. The fieldwork was carried out in 2018 and 2019 in Culiacán, Sinaloa. A qualitative approach was made to the sociocultural understanding of drug trafficking. We interviewed 16 young persons between the ages of 18 to 29 who collaborated or were involved with drug trafficking. The results highlight drug trafficking entrance motivation and experience; permanence, risk assessment, and consequences of the activities carried out; making plans within and outside drug trafficking. It is concluded that young people join and remain in drug trafficking due to the idealization of a successful life, economic retribution, acceptance, legitimacy, and recognition of drug trafficking in the context. For young people, drug trafficking is constituted as a space for work, projection, and building a future.
Testing the forecasting skills of aftershock models using a Bayesian framework
Authors: Dong Elisa , Shcherbakov Robert , Goda Katsuichiro
Published: June 2023

The Epidemic Type Aftershock Sequence (ETAS) model and the modified Omori law (MOL) are two aftershock rate models that are used for operational earthquake/aftershock forecasting. Previous studies have investigated the relative performance of the two models for specific case studies. However, a rigorous comparative evaluation of the forecasting performance of the basic aftershock rate models for several different earthquake sequences has not been done before. In this study, forecasts of five prominent aftershock sequences from multiple catalogs are computed using the Bayesian predictive distribution, which fully accounts for the uncertainties in the model parameters. This is done by the Markov Chain Monte Carlo (MCMC) sampling of the model parameters and forward simulation of the ETAS or MOL models to compute the aftershock forecasts. The forecasting results are evaluated using five different statistical tests, including two comparison tests. The forecasting skill tests indicate that the ETAS model tends to perform consistently well on the first three tests. The MOL fails the same tests for certain forecasting time intervals. However, in the comparison tests, it is not definite whether the ETAS model is the better performing model. This work demonstrates the use of forecast testing for different catalogs, which is also applicable to catalogs with a higher magnitude of completeness.
An overview of the material science and knowledge of nanomedicine, bioscaffolds, and tissue engineering for tendon restoration
Authors: Liang Wenqing , Zhou Chao , Meng Yanfeng , Fu Lifeng , Zeng Bin , Liu Zunyong , Ming Wenyi , Long Hengguo
Published: June 2023

Tendon wounds are a worldwide health issue affecting millions of people annually. Due to the characteristics of tendons, their natural restoration is a complicated and lengthy process. With the advancement of bioengineering, biomaterials, and cell biology, a new science, tissue engineering, has developed. In this field, numerous ways have been offered. As increasingly intricate and natural structures resembling tendons are produced, the results are encouraging. This study highlights the nature of the tendon and the standard cures that have thus far been utilized. Then, a comparison is made between the many tendon tissue engineering methodologies proposed to date, concentrating on the ingredients required to gain the structures that enable appropriate tendon renewal: cells, growth factors, scaffolds, and scaffold formation methods. The analysis of all these factors enables a global understanding of the impact of each component employed in tendon restoration, thereby shedding light on potential future approaches involving the creation of novel combinations of materials, cells, designs, and bioactive molecules for the restoration of a functional tendon.
Injectable antibacterial Ag-HA/ GelMA hydrogel for bone tissue engineering
Authors: Wang Jiapu , Wang Xuefeng , Liang Ziwei , Lan Weiwei , Wei Yan , Hu Yinchun , Wang Longfei , Lei Qi , Huang Di
Published: June 2023

Background: Fracture or bone defect caused by accidental trauma or disease is a growing medical problem that threats to human health.Currently, most orthopedic implant materials must be removed via follow-up surgery, which requires a lengthy recovery period and may result in bacterial infection. Building bone tissue engineering scaffolds with hydrogel as a an efficient therapeutic strategy has outstanding bionic efficiency.By combining some bionic inorganic particles and hydrogels to imitate the organic-inorganic characteristics of natural bone extracellular matrix, developing injectable multifunctional hydrogels with bone tissue repair effects and also displaying excellent antibacterial activity possesses attractive advantages in the field of minimally invasive therapy in clinical.

Methods: In the present work, a multifunctional injectable hydrogel formed by photocrosslinking was developed by introducing hydroxyapatite (HA) microspheres to Gelatin Methacryloyl (GelMA) hydrogel.

Results: The composite hydrogels exhibited good adhesion and bending resistance properties due to the existence of HA. In addition, when the concentration of GelMA is 10% and the concentration of HA microspheres is 3%, HA/GelMA hydrogel system displayed increased microstructure stability, lower swelling rate, increased viscosity, and improved mechanical properties. Furthermore, the Ag-HA/GelMA demonstrated good antibacterial activity against Staphylococcus aureus and Escherichia coli, which could signifificantly lower the risk of bacterial infection following implantation. According to cell experiment, the Ag-HA/GelMA hydrogel is capable of cytocompatibility and has low toxicity to MC3T3 cell.

Conclusion: Therefore, the new photothermal injectable antibacterial hydrogel materials proposed in this study will provide a promising clinical bone repair strategy and is expected to as a minimally invasive treatment biomaterial in bone repair fields.
I-125 seeds brachytherapy combined with immunotherapy for MET amplification in non-small cell lung cancer from clinical application to related lncRNA mechanism explore: a case report
Authors: Yang Mingxing , You Yuanli , Wang Xiuqing , Dong Wen
Published: June 2023

Advanced non-small cell lung cancer (NSCLC) with MET amplification primarily relies on MET inhibitors for treatment, but once resistance occurs, the available treatment options are limited and the prognosis is typically poor. A 57-year-old man with advanced NSCLC and C-MET amplification was initially treated with crizotinib but developed progressive disease. After the antirotinib treatment, he achieved a partial response for a year. Genetic testing showed high PD-L1 expression, and he was treated with pembrolizumab and chemotherapy for 3 months, with partial response. Maintenance therapy with pembrolizumab and local I-125 seeds brachytherapy (ISB) was given after the lung lesion progressed but other lesions remained stable. The therapy resulted in significant resolution of the right upper lung lesion. It demonstrates the effectiveness of ISB-ICI combination in treating MET amplification advanced NSCLC. Ongoing research and treatment innovation are important in managing advanced NSCLC with complex genetic aberrations. To explore the candidate mechanism of ISB therapy response, we download public genetic data and conduct different expression Lncrnas analysis and pathway analysis to discover radiotherapy related sensitive or resistance lncRNAs and pathways, we found that AL654754.1 is a key lncRNA with radiotherapy response, and it also include in classical p53 and Wnt signaling pathway. Overall, the clinical case reports, combined with the exploration of underlying mechanisms, provide positive guidance for the precise treatment of lung cancer.
Atopic dermatitis-derived Staphylococcus aureus strains: what makes them special in the interplay with the host
Authors: Conte Antonietta Lucia , Brunetti Francesca , Marazzato Massimiliano , Longhi Catia , Maurizi Linda , Raponi Giammarco , Palamara Anna Teresa , Grassi Sara , Conte Maria Pia
Published: June 2023

Background

Atopic dermatitis (AD) is a chronic inflammatory skin condition whose pathogenesis involves genetic predisposition, epidermal barrier dysfunction, alterations in the immune responses and microbial dysbiosis. Clinical studies have shown a link between Staphylococcus aureus and the pathogenesis of AD, although the origins and genetic diversity of S. aureus colonizing patients with AD is poorly understood. The aim of the study was to investigate if specific clones might be associated with the disease.

Methods

WGS analyses were performed on 38 S. aureus strains, deriving from AD patients and healthy carriers. Genotypes (i.e. MLST, spa-, agr- and SCCmec-typing), genomic content (e.g. virulome and resistome), and the pan-genome structure of strains have been investigated. Phenotypic analyses were performed to determine the antibiotic susceptibility, the biofilm production and the invasiveness within the investigated S. aureus population.

Results

Strains isolated from AD patients revealed a high degree of genetic heterogeneity and a shared set of virulence factors and antimicrobial resistance genes, suggesting that no genotype and genomic content are uniquely associated with AD. The same strains were characterized by a lower variability in terms of gene content, indicating that the inflammatory conditions could exert a selective pressure leading to the optimization of the gene repertoire. Furthermore, genes related to specific mechanisms, like post-translational modification, protein turnover and chaperones as well as intracellular trafficking, secretion and vesicular transport, were significantly more enriched in AD strains. Phenotypic analysis revealed that all of our AD strains were strong or moderate biofilm producers, while less than half showed invasive capabilities.

Conclusions

We conclude that in AD skin, the functional role played by S. aureus may depend on differential gene expression patterns and/or on post-translational modification mechanisms rather than being associated with peculiar genetic features.
Agile methodologies between software development and music production: an empirical study
Authors: Ciancarini Paolo , Ergasheva Shokhista , Farina Mirko , Mubarakshin Damir , Succi Giancarlo
Published: June 2023

Over the past 20 years agile methodologies revolutionized Information Technology, offering tremendous opportunities for the development of Software Engineering as an independent discipline. More specifically, agile methodologies contributed to enhancing the effectiveness and the speed of the production process as well as to improving the productivity and motivations of software developers organized in high performing teams. The agile philosophy can be and has been applied in different contexts and across several domains. This work analyses the relationship between Agile methodologies used by software engineers and the practices pursued by musicians in their daily lives. Our findings suggests that collaborative, strongly planned software development life cycle models (such as Waterfall, V-model, iterative, and Spiral) are not adequate models to describe the daily practices of musical composers. This is because their work requires a lot of flexibility, which such models intrinsically lack, because they are oriented to ensuring some form of monitorable progress. Interestingly, our findings also show that nine out of 12 Agile Principles are consciously or unconsciously followed by musicians in their practices. This suggests that there are some deep connections between these twoprima faciedifferent fields, which are both very creative. Even though our findings await replication, possibly with larger statistical samples, they contribute to open up a new strand of research in the field.
Remote monitoring of implantable loop recorders reduces time to diagnosis in patients with unexplained syncope: a multicenter propensity score-matched study
Authors: Russo Vincenzo , Rago Anna , Grimaldi Nicola , Chianese Raffaele , Viggiano Aniello , D’Alterio Giuliano , Colonna Diego , Mattera Iacono Agostino , Papa Andrea Antonio , Spadaro Guerra Andrea , Gargaro Alessio , Rapacciuolo Antonio , Sarubbi Berardo , D’Onofrio Antonio , Nigro Gerardo
Published: June 2023

Background

There are little data on remote monitoring (RM) of implantable loop recorders (ILRs) in patients with unexplained syncope and whether it confers enhanced diagnostic power.

Objective

To evaluate the effect of RM in ILR recipients for unexplained syncope for early detection of clinically relevant arrhythmias by comparison with a historical cohort with no RM.

Methods

SyncRM is a propensity score (PS)-matched study prospectively including 133 consecutive patients with unexplained syncope and ILR followed up by RM (RM-ON group). A historical cohort of 108 consecutive ILR patients with biannual in-hospital follow-up visits was used as control group (RM-OFF group). The primary endpoint was the time to the clinician's evaluation of clinically relevant arrhythmias (types 1, 2, and 4 of the ISSUE classification).

Results

The primary endpoint of arrhythmia evaluation was reached in 38 patients (28.6%) of the RM-ON group after a median time of 46 days (interquartile range, 13–106) and in 22 patients (20.4%) of the RM-OFF group after 92 days (25–368). The PS-matched adjusted ratio of rates of arrhythmia evaluation was 2.53 (95% confidence interval, 1.32–4.86) in the RM-ON vs. RM-OFF group (p = 0.005).

Conclusion

In our PS-matched comparison with a historical cohort, RM of ILR patients with unexplained syncope was associated with a 2.5-fold higher chance of evaluations of clinically relevant arrhythmias as compared with biannual in-office follow-up visits.
Acute stroke CDS: automatic retrieval of thrombolysis contraindications from unstructured clinical letters
Authors: Cutforth Murray , Watson Hannah , Brown Cameron , Wang Chaoyang , Thomson Stuart , Fell Dickon , Dilys Vismantas , Scrimgeour Morag , Schrempf Patrick , Lesh James , Muir Keith , Weir Alexander , O’Neil Alison Q
Published: June 2023

Introduction

Thrombolysis treatment for acute ischaemic stroke can lead to better outcomes if administered early enough. However, contraindications exist which put the patient at greater risk of a bleed (e.g. recent major surgery, anticoagulant medication). Therefore, clinicians must check a patient's past medical history before proceeding with treatment. In this work we present a machine learning approach for accurate automatic detection of this information in unstructured text documents such as discharge letters or referral letters, to support the clinician in making a decision about whether to administer thrombolysis.

Methods

We consulted local and national guidelines for thrombolysis eligibility, identifying 86 entities which are relevant to the thrombolysis decision. A total of 8,067 documents from 2,912 patients were manually annotated with these entities by medical students and clinicians. Using this data, we trained and validated several transformer-based named entity recognition (NER) models, focusing on transformer models which have been pre-trained on a biomedical corpus as these have shown most promise in the biomedical NER literature.

Results

Our best model was a PubMedBERT-based approach, which obtained a lenient micro/macro F1 score of 0.829/0.723. Ensembling 5 variants of this model gave a significant boost to precision, obtaining micro/macro F1 of 0.846/0.734 which approaches the human annotator performance of 0.847/0.839. We further propose numeric definitions for the concepts of name regularity (similarity of all spans which refer to an entity) and context regularity (similarity of all context surrounding mentions of an entity), using these to analyse the types of errors made by the system and finding that the name regularity of an entity is a stronger predictor of model performance than raw training set frequency.

Discussion

Overall, this work shows the potential of machine learning to provide clinical decision support (CDS) for the time-critical decision of thrombolysis administration in ischaemic stroke by quickly surfacing relevant information, leading to prompt treatment and hence to better patient outcomes.
A new Bayesian-based brittleness prediction method for the Pingdiquan formation in the Eastern Shiqiantan depression of the East Junggar Basin
Authors: Liu Zhen , Zhang Junhua , Sun Youzhuang , Yu Zhengjun , Ren Ruijun
Published: June 2023

This paper presents a novel Bayesian-based method for predicting brittleness. The method involves synthesizing petrophysical data from multiple well cores to establish a joint Gaussian distribution function for shale facies and non-shale facies. Furthermore, Bayesian facies classification is applied to seismic data. The proposed method combines non-shale facies data with Rickman brittleness data to obtain a new brittleness index. The joint Gaussian distribution function and Bayesian classification are utilized to enhance the differentiation of brittleness among different geological bodies. Practical data analysis demonstrates that the new brittleness index effectively increases the contrast in brittleness values between various geological bodies, highlighting target areas of interest. The presented method offers a promising approach for brittleness prediction, leveraging the integration of petrophysical and seismic data through Bayesian techniques. The results suggest its potential applicability in enhancing the characterization and understanding of geological formations.
Mapping the impacts of the anthropogenic activities and seawater intrusion on the shallow coastal aquifer of Port Said, Egypt
Authors: Abdelfattah Mohamed , Abdel-Aziz Abu-Bakr Heba , Aretouyap Zakari , Sheta Mariam Hassan , Hassan Taher Mohammed , Geriesh Mohamed H. , Shaheen Shams El-Din , Alogayell Haya M. , M. EL-Bana Eman Mohamed , Gaber Ahmed
Published: June 2023

The quality and quantity of groundwater resources have been continuously deteriorating as a result of anthropogenic activities and their excessive usage. This has intensified seawater intrusion, particularly in the coastal area of Egypt. The management of this issue and preventing ongoing groundwater contamination are crucial responsibilities. Thus, an integrated strategy using remote sensing, geophysical technique, and hydrogeochemical analysis is used in this work to identify the causes of degradation and evaluate their impacts on the groundwater quality in East Port Said, Egypt. The following points were identified: 1) Remote sensing analysis between 1984 and 2015 showed an increase in anthropogenic activities, such as the construction of fish farms and vegetation, which became their areas of 12.5 and 37.8 km2 respectively. 2) Field observations demonstrated that the groundwater resources are being overexploited and it is expected that these human activities could have an impact on the groundwater quality. 3) The results of the resistivity approach indicated that sand and clay constitute the underlying layers and the shallow subsurface strata contain a high concentration of saline water. As a result, the aquifer is vulnerable to seawater intrusion due to its homogeneity. 4) Nineteen samples of groundwater were collected from the shallow Quaternary aquifer and the hydrochemical characteristic of the samples was identified. The hydrochemical analysis showed that the groundwater across the research area is of the Na-Cl water type and is highly saline (from 7,558 to 23,218 mg/L). By integrating the aforementioned techniques, it is evident that the research region is affected by anthropogenic activities as well as seawater intrusion on groundwater quality. These results serve as a solid base for further research on groundwater-surface water interactions and the evaluation of possible sources of contamination in the shallow aquifers under stress from anthropogenic activity in such environments.
Germline loss-of-function PAM variants are enriched in subjects with pituitary hypersecretion
Authors: Trivellin Giampaolo , Daly Adrian F. , Hernández-Ramírez Laura C. , Araldi Elisa , Tatsi Christina , Dale Ryan K. , Fridell Gus , Mittal Arjun , Faucz Fabio R. , Iben James R. , Li Tianwei , Vitali Eleonora , Stojilkovic Stanko S. , Kamenicky Peter , Villa Chiara , Baussart Bertrand , Chittiboina Prashant , Toro Camilo , Gahl William A. , Eugster Erica A. , Naves Luciana A. , Jaffrain-Rea Marie-Lise , de Herder Wouter W. , Neggers Sebastian JCMM , Petrossians Patrick , Beckers Albert , Lania Andrea G. , Mains Richard E. , Eipper Betty A. , Stratakis Constantine A.
Published: June 2023

Introduction

Pituitary adenomas (PAs) are common, usually benign tumors of the anterior pituitary gland which, for the most part, have no known genetic cause. PAs are associated with major clinical effects due to hormonal dysregulation and tumoral impingement on vital brain structures. PAM encodes a multifunctional protein responsible for the essential C-terminal amidation of secreted peptides.

Methods

Following the identification of a loss-of-function variant (p.Arg703Gln) in the peptidylglycine a-amidating monooxygenase (PAM) gene in a family with pituitary gigantism, we investigated 299 individuals with sporadic PAs and 17 familial isolated PA kindreds for PAM variants. Genetic screening was performed by germline and tumor sequencing and germline copy number variation (CNV) analysis.

Results

In germline DNA, we detected seven heterozygous, likely pathogenic missense, truncating, and regulatory SNVs. These SNVs were found in sporadic subjects with growth hormone excess (p.Gly552Arg and p.Phe759Ser), pediatric Cushing disease (c.-133T>C and p.His778fs), or different types of PAs (c.-361G>A, p.Ser539Trp, and p.Asp563Gly). The SNVs were functionally tested in vitro for protein expression and trafficking by Western blotting, splicing by minigene assays, and amidation activity in cell lysates and serum samples. These analyses confirmed a deleterious effect on protein expression and/or function. By interrogating 200,000 exomes from the UK Biobank, we confirmed a significant association of the PAM gene and rare PAM SNVs with diagnoses linked to pituitary gland hyperfunction.

Conclusion

The identification of PAM as a candidate gene associated with pituitary hypersecretion opens the possibility of developing novel therapeutics based on altering PAM function.
Scrutiny of power grids by penetrating PV energy in wind farms: a case study of the wind corridor of Jhampir, Pakistan
Authors: Soomar Arsalan Muhammad , Guanghua Lyu , Shaikh Shoaib , Shah Syed Hadi Hussain , Musznicki Piotr
Published: June 2023

This study examines the problems caused by intermittent renewable energy sources, especially wind farms, and suggests a different solar energy penetration strategy to improve their loading capacity. The study uses real-time data from a wind farm in Jhampir, Pakistan, to analyse and assess various aspects of grid stations connected to wind farms. Electrical Transient Analyzer Program is used to validate the results by linking these with actual grid system. The article focuses on creating a model for a grid connected to a wind farm and the simulation of outcomes following capacity expansion, with the installation of an autotransformer. The original capacity of the wind farm was 750 MW, which was increased to 1,250 MW, i.e., 1.66 times the actual capability. Furthermore, this capacity was further enhanced to 1,540 MW, which becomes 1.23 times the previous capacity by the penetration of a photovoltaic power plant.
TCN-GAT multivariate load forecasting model based on SHAP value selection strategy in integrated energy system
Authors: Zheng Qianyun , Zheng Jianyong , Mei Fei , Gao Ang , Zhang Xi , Xie Yang
Published: June 2023

Load forecasting is an important prerequisite and foundation for ensuring the rational planning and safe operation of integrated energy systems. In view of the interactive coupling problem among multivariate loads, this paper constructs a TCN-GAT multivariate load forecasting model based on SHAP (Shapley Additive Explanation) value selection strategy. The model uses temporal convolutional networks (TCN) to model the multivariate load time series of the integrated energy system, and applies the global attention mechanism (GAT) to process the output of the network hidden layer state, thereby increasing the weight of key features that affect load changes. The input variables are filtered by calculating the SHAP values of each feature, and then returned to the TCN-GAT model for training to obtain multivariate load forecasting results. This can remove the interference of features with low correlation to the model and improve the forecasting effect. The analysis results of practical examples show that compared with other models, the TCN-GAT multivariate load forecasting model based on SHAP value selection strategy proposed in this paper can further reduce the forecasting error and has better forecasting accuracy and application value.