bluuebunny/crossref_metadata_2025 huggingface dataset:
Joint Processing of Aerospace and Ground Surveying Materials to Create 3D Terrain Models
Authors: Mamazhakypova G. , Baiyshbekkyzy G.
Published: May 2024

Methods for creating three-dimensional terrain models based on aerospace and ground survey data is an important direction in the field of geographic information technologies. This article presents a detailed analysis of the process of developing and implementing an automated approach to creating 3D terrain models using Agisoft Metashape and ArcGIS software. The presented method can significantly reduce the time and human resources required to create 3D models, as well as increase the accuracy and reliability of the results obtained.
Organic Agriculture in Turkey and the World
Authors: Karabacak M. , Yılmaz G.
Published: May 2024

Organic agriculture is an alternative production system that supports soil structure, fertility and conservation by protecting the ecological balance, using biological control methods against diseases and pests; based on the sustainability of living life, aiming for the highest yield with the maximum use of natural energy resources. In this context, it covers a different production process from conventional agriculture. While intensive input applications in conventional agriculture aim for the highest yield, organic agriculture prioritizes sustainability. Sustainable agriculture is defined as an agricultural practice that protects soil, water and biodiversity while meeting food, energy and natural resource needs. To ensure that organic agriculture contributes to sustainability goals, organic regulations need to focus more on environmental and best practices. Increasing demands day by day have positively affected the sustainability of organic agriculture. The common feature of most of the definitions of organic agriculture is that the products should be produced in accordance with the basic standards and that every stage from the land to the table is regularly controlled. The fact that every stage of organic agriculture is audited and documented, and that the practices operate in accordance with legal procedures are other noteworthy elements. These legal procedures provide protection at many points. Organic agriculture is not only limited to safe food production to protect human health; it is also an agricultural and ecosystem management that is environmentally friendly, effective in reducing ecological pollution, protection of water resources, prevention of erosion, protection of biodiversity, agricultural sustainability and agricultural development.
Investigation of Metabolic Responses in Virus-infected Wheat (Triticum aestivum L.) Grown in Azerbaijan
Authors: Sultanova N. , Gurbanova U. , Bayramova N. , Huseynova I.
Published: May 2024

Barley yellow dwarf viruses (BYDV), Wheat streak mosaic virus (WSMV), Wheat dwarf virus (WDV), and Soil-borne wheat mosaic virus (SBWMV) constitute the most dangerous and widespread viruses affecting cereals across all wheat-growing regions worldwide. While numerous foreign scientific reports focus on the impact of climate change on plants, pathogens, and their vectors, only a limited number of studies address cereal viruses. The objective of this study was to investigate the variability of economically significant wheat viruses in several regions of Azerbaijan and to identify certain metabolic enzymes such as malate dehydrogenase (MDH), aspartate aminotransferase (AsAT), and alanine aminotransferase (ALAT) in virus-infected wheat samples. During 2022-2023, it was observed that BYDV and WSMV alternated in prevalence from year to year, with no instances of co-infection recorded. SBWMV was not detected in the tested samples. WSMV incidence rates were 12.4% and 19% in 2022 and 2023, respectively, whereas BYDV was detected at rates of 14.6% and 8.5%, respectively. Our results support a tight relationship between amino acid metabolism and stress responses. The results of the research allow the conclusion that mitochondrial NAD-MDH implements oxidation of NADH reduced in the reaction of glycine decarboxylation during photorespiration and intensifies biosynthesis of 2-oxoglutarate playing the role of the carbon skeleton in the synthesis of amino acids.
Taxonomic Relationship of Wide Pests in Sugar Beet Agrocenosis
Authors: Gazi S. , Shahverdiyeva Z. , Rzayeva G. , Gubadova M.
Published: May 2024

Based on the conducted research, information on the species composition of pests that spread in the agricultural farms of Imishli and Agdash districts of the Republic, where sugar beet is grown, as well as in the private backyards of the population, was reflected in the article. During extensive research conducted in stationary areas and surrounding areas, it was determined that pests belonging to the groups of Coleoptera, Hemiptera, Orthoptera, Diptera and Lepidoptera are found in the agrocenosis of sugar beet.
Establishing the Quality of Buckwheat Grain Using Non-destructive Testing Method
Authors: Tarasova E. , Guryeva K. , Xhaba N. , Sokolova T.
Published: May 2024

The assessment of the quality of buckwheat grain according to regulatory indicators does not allow to determine the state of its core. The paper examines the possibility of using microfocus radiography as a non-destructive testing method. The latent (internal) defects of buckwheat grain (underdeveloped (unfulfilled) grain, hollow grain, damaged core (fracturing), damaged core (destruction), grain with a damaged shell) were identified and classified, obvious distinguishing features on the radiographic image of the grain are described. It is shown that when conducting a comprehensive assessment, the results of the X-ray examination make it possible to increase the objectivity of the conclusion on the quality of buckwheat grain.
Phytotherapy for stress in children from the age of three
Authors: Ilyenko L. I. , Orlova N. V.
Published: May 2024

Mental and behavioral disorders begin in early childhood, affect the quality of life of children and their parents, can lead to changes in brain development and function, and somatic diseases. Stress in young children can be associated with frightening changes in their lives – going to kindergarten, school. Treatment of mental disorders in childhood requires a comprehensive approach, including elimination of the causes of disorders, lifestyle modification, psychological and medical support. Phytotherapy is an effective treatment method accepted in most countries and characterized by a low risk of adverse events, which makes it indispensable in pediatric practice. The article describes the characteristics of medicinal plants used for the treatment of mental disorders. A review of clinical studies confirming the efficacy and safety of phytotherapy is presented. A tactical approach to the use of medicinal plant-based drugs in the practice of a pediatrician is considered.
CASES OF ISOLATED SKIN AND NAIL ASPERGILLOSIS AND SECONDARY ASPERGILLOUS SKIN LESION CAUSED BY AN OCCUPATIONAL FACTOR. SYMPTOM OF «DRIED LEAVES» FOR NAIL ASPERGILLOSIS
Authors:   Poltava State Medical University, V.G. Kravchenko , A.V. Kravchenko ,   Kharkiv National Medical University
Published: May 2024

Cases of aspergillosis (A.), both isolated lesions of the skin and nails, and secondary skin lesions as a result of complications of invasive aspergillosis of the lungs and intestines, can be considered occupational, since there is a clear connection with the direct professional activities of the patients. it was found. It can be assumed that A. of the skin and nails in dermatological practice occurs more often than is diagnosed, especially since at the present stage the total number of patients with fungal infection is increasing. The goal is to share the experience of clinical and laboratory diagnostics and effective treatment of patients with isolated lesions of the skin and nails due to aspergillosis. Materials and methods. A clinical case of skin damage due to invasive aspergillosis (lungs and intestines) is presented, as both cases are caused by professional factors. Patients were examined in detail with the use of microscopic and mycological (sowing material on a nutrient medium) research. Results and conclusions. When diagnosing, the professional background of the patients should be taken into account. The clinical «symptom of dried leaves» proposed by us is likely to be of significant importance in establishing the clinical diagnosis of onychomycosis caused by A.flavus, and which can serve as a differential sign for other fungal infections of the nails. Our experience confirms the effectiveness of treating A. skin and nails using the «pulse therapy» method with Orungal and confirms its increase under the condition of a combination of systemic action and local antifungal activity of the drug Cidipol.
Utilization of graphene as an alternative sustainable amendment in improving soil health through accelerated decomposition of oil palm mulch and enhanced nutrient availability
Authors: Khomphet Thanet , Hussain Tajamul
Published: May 2024

Graphene has unique properties for improving soil health properties such as nutrient availability, soil physical and chemical properties, and controlled release of essential elements. This research aimed at determining the impact of graphene amendment on the decomposition of oil palm frond mulching and on soil health status. The study was conducted using a factorial experiment in completely randomized design with two main factors: (i) covering conditions: cover with plastic sheet and no cover, and (ii) graphene application that included T1 (control): oil palm frond mulching (OFM), T2: OFM + graphene (G), T3: OFM + G + chemical fertilizer, and T4: OFM + G + goat manure. The results indicated that there were significant differences among graphene applications, between cover conditions, and in interactions between graphene applications and cover conditions for all soil characteristics in the most observed month. In the third month of soil analysis, the treatment of graphene applications showed higher electrical conductivity (T2: 151.7 ± 6.8 µS cm−1), available phosphorus (T3: 9.0 ± 6.7 mg kg−1), exchangeable potassium (T2: 67.1 ± 24.9 mg kg−1), and exchangeable calcium (T3: 95.4 ± 5.1 mg kg−1), compared to control. The cover condition showed suitable soil pH (5.0 ± 0.2), higher soil available phosphorus (7.1 ± 5.0 mg kg−1), and exchangeable calcium (599.1 ± 235.2 mg kg−1), but the no-cover condition presented higher soil organic matter (0.7% ± 0.2%), exchangeable potassium (60.3 ± 19.1 mg kg−1), and exchangeable magnesium (96.7 ± 11.4 mg kg−1). Correlation results indicated that most soil characteristics were correlated under graphene applications. Principal component analysis showed that the treatments of graphene application dominated most soil characteristics. The results suggest that graphene has potential for improving soil health properties and can be applied as an alternative sustainable amendment to accelerate the decomposition of oil palm frond mulch and enhance nutrient availability for oil palm. In addition, the authors suggest that further investigations should consider more soil health parameters in long-term field studies for a better understanding and to provide recommendations to farmers.
Optimization of electroporation method and promoter evaluation for type-1 methanotroph, Methylotuvimicrobium alcaliphilum
Authors: Goswami Shubhasish , Singer Steven W. , Simmons Blake A. , Awasthi Deepika
Published: May 2024

Methanotrophic bacteria are promising hosts for methane bioconversion to biochemicals or bioproducts. However, due to limitations associated with long genetic manipulation timelines and, lack of choice in genetic tools required for strain engineering, methanotrophs are currently not employed for bioconversion technologies. In this study, a rapid and reproducible electroporation protocol is developed for type 1 methanotroph, Methylotuvimicrobium alcaliphilum using common laboratory solutions, analyzing optimal electroshock voltages and post-shock cell recovery time. Successful reproducibility of the developed method was achieved when different replicative plasmids were assessed on lab adapted vs. wild-type M. alcaliphilum strains (DASS vs. DSM19304). Overall, a ∼ 3-fold decrease in time is reported with use of electroporation protocol developed here, compared to conjugation, which is the traditionally employed approach. Additionally, an inducible (3-methyl benzoate) and a constitutive (sucrose phosphate synthase) promoter is characterized for their strength in driving gene expression.
Genetic evidence strengthens the connection between gut microbiota and gingivitis: a two-sample Mendelian randomization study
Authors: Hang Zhou , Rouyi Chen , Sen Li
Published: May 2024

Introduction

The oral cavity and gut tract, being interconnected and rich in microbiota, may have a shared influence on gingivitis. However, the specific role of distinct gut microbiota taxa in gingivitis remains unexplored. Utilizing Mendelian Randomization (MR) as an ideal method for causal inference avoiding reverse causality and potential confounding factors, we conducted a comprehensive two-sample MR study to uncover the potential genetic causal impact of gut microbiota on gingivitis.

Methods

Instrumental variables were chosen from single nucleotide polymorphisms (SNPs) strongly associated with 418 gut microbiota taxa, involving 14,306 individuals. Gingivitis, with 4,120 cases and 195,395 controls, served as the outcome. Causal effects were assessed using random-effect inverse variance-weighted, weighted median, and MR-Egger methods. For replication and meta-analysis, gingivitis data from IEU OpenGWAS were employed. Sensitivity analyses included Cochran’s Q tests, funnel plots, leave-one-out analyses, and MR-Egger intercept tests. This study aimed to assess the genetic correlation between the genetically predicted gut microbiota and gingivitis using linkage disequilibrium score regression (LDSC).

Results

Three gut microbiota taxa (class Actinobacteria id.419, family Defluviitaleaceae id.1924, genus Defluviitaleaceae UCG011 id.11287) are predicted to causally contribute to an increased risk of gingivitis (P< 0.05). Additionally, four gut microbiota taxa (class Actinobacteria id.419, genus Escherichia Shigella id.3504, genus Ruminococcaceae UCG002 id.11360) potentially exhibit inhibitory causal effects on the risk of gingivitis (P< 0.05). No significant evidence of heterogeneity or pleiotropy is detected. Our findings indicate a suggestive genetic correlation between class Actinobacteria id.419, class Bacteroidia id.912, family Defluviitaleaceae id.1924, genus Escherichia Shigella id.3504 and gingivitis.

Conclusion

Our study establishes the genetic causal effect of 418 gut microbiota taxa on gingivitis, offering insights for clinical interventions targeting gingivitis. Subsequent research endeavors are essential to corroborate the findings of our present study.
Disinformation and health: fact-checking strategies of Spanish health public institutions through YouTube
Authors: Vázquez-Gestal Montse , Pérez-Seoane Jesús , Fernández-Souto Ana-Belén
Published: May 2024

Introduction

Through their social media, public institutions address fake news and tackle disinformation that affects both them and the general public. With the rise of online video platforms, the audiovisual format has made its way as a dynamic and engaging format that allows for the creation of narratives to counter distorted information. This circumstance is particularly noticeable in the healthcare sector, where most of the recent fact-checking activity is taking place.

Methodology

Through the analysis of the official YouTube channels of the health departments of all Spanish autonomous communities from 1 January 2021 to 31 December 2023, this paper explores the use of audiovisual fact-checking by Spanish regional governments, and it describes the nature of these videos and the strategies undertaken on that platform to tackle disinformation.

Results

Despite the expected territorial disparities, the research findings show that an increasing number of Spanish regional health authorities are using YouTube to fight disinformation. They also show that the videos posted on that platform provide a strong response to fake news, either as a preventive measure or in response to existing publications.

Discussion

In the current landscape of declining mass media, where audience dispersion leads a to (sometimes deliberate) fragmentation of discourse, we are confronted with the paradox of being a society with access to a vast amount of information, but not well-informed. Therefore, it is essential to have strong institutions that verify fake content through popular formats.
The effect of structural preservation conditions on pore structure of marine shale reservoir: a case study of the Wufeng-Longmaxi Formation shale, Southern Sichuan Basin, China
Authors: Yu Weiming , Yuan Shusheng , Tang Haoxuan , Luo Chao , Wu Wei , Liu Jia , Yang Yuran , Zhong Kesu , He Liang
Published: May 2024

The marine shale within the Sichuan Basin constitutes China’s significant shale gas production, featuring old formation age, high degree of thermal evolution, multiple tectonic movements, and complex structural conditions. However, there are significant differences in the shale gas preservation conditions and reservoir quality in different areas, limiting future large-scale exploration and development. Pore structure significantly influences shale reservoir quality, gas content, and exploration of shale gas occurrence, migration, and enrichment mechanisms. The influence of structural-dominated preservation conditions on shale pore structures is essential to comprehend for effective shale gas exploitation. This study employs field-emission scanning electron microscopy in conjunction with other techniques (low-temperature N2 adsorption, low-temperature CO2 adsorption, and nuclear magnetic resonance) for detailed analyses of the pore structure across varied structural zones, revealing the influence of structural attributes, fault systems, depth of burial, and formation pressure on pore architecture, and examining the relationship between pore structure and shale gas preservation conditions. The results show that stable structural condition is conducive to the development and preservation of shale pores. Structural compression causes inorganic and organic pores to become narrow and elongated due to shrinkage, with a significant increase in microfractures. The porosity of shale with stable structural conditions exhibits markedly increased porosity compared to samples under structural compressions. Under conditions of similar TOC and mineral composition, the pore size distribution (PSD), pore volume (PV), and specific surface area (SSA) of shale after structural compression are significantly lower than those of samples with stable structural conditions. As the burial depth increases, the shale porosity shows a decreasing trend, but the decrease is limited. Burial depth significantly impacts the SSA and PV of high-TOC samples (3%–6%). As the burial depth increases, both SSA and PV show a significant decreasing trend. When the burial depth reaches 4000 m, SSA and PV tend to concentrate. The formation pressure coefficient is an important factor for the development and preservation of shale pores, and porosity is positively correlated with the formation pressure coefficient. Increased formation pressure coefficient indicates superior preservation conditions and enhanced pore development.
Cross-cultural communication across STEM disciplines
Authors: Ma Taejung , Lucietto Anne M.
Published: May 2024

Collaboration across cultures in Science, Technology, Engineering, and Math (STEM) fields has become increasingly common. This means many STEM professionals in US-based companies now work with professionals from other cultures. In this global context, cross-cultural knowledge is crucial for effective communication. Without this knowledge, there can be misunderstandings and discomfort. Different cultures use different communication styles, which has been studied by the field of communication. The concept of high and context low-context culture is used to discuss the role of contextual cues in communication. High-context cultures rely on implicit language and contextual and situational cues, while low-context cultures use explicit language. Although no culture is strictly high or low context, this concept is important as it helps us understand how our communication style can be influenced by cultural characteristics. There is a growing need to support and encourage students to become interested in pursuing careers in STEM fields. Many STEM programs in the United States have emphasized the importance of collaboration to meet this need. However, effective collaboration requires effective communication, and students often lack opportunities to explore how they can communicate effectively with collaborators from other cultures and countries. To bridge this gap, this article proposes incorporating communication into STEM courses for undergraduate students. The course will include learning opportunities about low and high-context cultures and guest speakers. This article aims to provide insights into how communication content can prepare undergraduate students to become culturally competent STEM professionals.
Gender and race disparities in the prevalence of chronic kidney disease among individuals with hypertension in the United States, 2001–2016
Authors: Shen Jing , Wang Baoquan , Jing Li , Chen Tiancong , Han Li , Dong Weiwei
Published: May 2024

Background

Chronic kidney disease (CKD) is a common complication among individuals with hypertension. We aimed to identify the prevalence of CKD and the sex and race disparities within the hypertensive population in the United States from 2001–2016.

Methods

A total of 16,148 participants with hypertension were included, representing 561,909,480 individuals from the U.S. population between 2001 and 2016, as documented in the National Health and Nutrition Examination Survey. The prevalence of albuminuria and CKD stage were assessed using survey-weighted general linear regression analysis. Heterogeneity in the CKD stage among the hypertensive population, stratified by sex and race, was identified through survey-weighted logistic regression analysis.

Results

Overall, the prevalence of albuminuria remained stable (p for trend = 0.3196), and changes in the CKD stage were minimal (p for trend > 0.05) from 2001–2016. In the analysis of CKD stage heterogeneity by sex and race, the prevalence of CKD was higher among women than men and higher among individuals of other races combined than non-Hispanic Whites, but the differences were not statistically significant.

Conclusion

The overall CKD stage within the hypertensive population plateaued between 2001 and 2016. Our findings highlight the importance of continuous monitoring and potential refinement of renoprotection strategies in individuals with hypertension to mitigate the persistent burden of CKD and address health disparities among different demographic groups.
Synergistic effect of lipoprotein(a) and high-sensitivity C-reactive protein on the risk of all-cause and cardiovascular death in patients with acute myocardial infarction: a large prospective cohort study
Authors: Wang Zhenwei , Tang Junnan , Shi Qian , Fang Lijuan , Liu Naifeng , Zhang Jinying
Published: May 2024

Objective

Although lipoprotein(a) [Lp(a)] and high-sensitivity C-reactive protein (Hs-CRP) are closely associated with the mortality of acute myocardial infarction (AMI), their synergistic effect on the risk of death remains unknown. Therefore, this study aimed to explore the combined effect of Lp(a) and Hs-CRP on the incidence of all-cause and cardiovascular death in AMI patients.

Methods

A comprehensive cohort study enrolled 912 AMI patients, categorizing them into four groups based on Lp(a) and Hs-CRP levels: Group 1 [Lp(a) < 30 mg/dL & Hs-CRP < 2 mg/L], Group 2 [Lp(a) < 30 mg/dL & Hs-CRP ≥ 2 mg/L], Group 3 [Lp(a) ≥ 30 mg/dL & Hs-CRP < 2 mg/L], and Group 4 [Lp(a) ≥ 30 mg/dL & Hs-CRP ≥ 2 mg/L]. Cox regression analysis, Kaplan-Meier survival analysis and sensitivity analysis were employed to determine the combined effects of Lp(a) and Hs-CRP on the risk of all-cause and cardiovascular death.

Results

Over a median observation period of 38.98 months, 217 patients passed away, with 137 deaths attributed to cardiovascular causes. The multivariate Cox regression analysis revealed that in the comprehensively adjusted Model 3, only Lp(a) and the combination of Lp(a) and Hs-CRP exhibited a strong association with cardiovascular death risk. Specifically, for Lp(a) levels ≥ 30 mg/dL compared to < 30 mg/dL, the hazard ratio (HR) was 2.434 with a 95% confidence interval (CI) of 1.653–3.583 (P < 0.001); for log10(Lp(a)), the HR was 2.630 with a 95% CI of 1.530–4.523 (P < 0.001); for Group 4 versus Group 1, the HR was 2.346 with a 95% CI of 1.054–5.220 (P = 0.037); and for Group 4 versus Groups 1 + 2 + 3, the HR was 1.878 with a 95% CI of 1.284–2.748 (P = 0.001). Sensitivity analysis indicated that the synergy between Lp(a) and Hs-CRP continued to be independently associated with the risk of cardiovascular death. For Group 3 versus Group 1, the HR was 3.353 with a 95% CI of 1.133–9.917 (P = 0.029); for Group 4 versus Group 1, the HR was 3.710 with a 95% CI of 1.466–9.392 (P = 0.006); and for Group 4 versus Groups 1 + 2 + 3, the HR was 2.433 with a 95% CI of 1.620–3.656 (P < 0.001).

Conclusions

Compared to elevated levels of either Lp(a) or Hs-CRP alone, the concurrent high levels of both significantly increased the risk of cardiovascular death in patients with AMI, underscoring the importance of considering their combined effects in the prognostic management of AMI patients.
Advancing air quality forecasting in Abu Dhabi, UAE using time series models
Authors: Ramadan Mona S. , Abuelgasim Abdelgadir , Al Hosani Naeema
Published: May 2024

This research enhances air quality predictions in Abu Dhabi by employing Autoregressive Integrated Moving Average (ARIMA) models on comprehensive air quality data collected from 2015 to 2023. We collected hourly data on nitrogen dioxide (NO2), particulate matter (PM10), and fine particulate matter (PM2.5) from 19 well-placed ground monitoring stations. Our approach utilized ARIMA models to forecast future pollutant levels, with extensive data preparation and exploratory analysis conducted in R. Our results found a significant drop in NO2 levels after 2020 and the highest levels of particulate matter observed in 2022. The findings of our research confirm the effectiveness of the models, indicated by Mean Absolute Percentage Error (MAPE) values ranging from 7.71 to 8.59. Additionally, our study provides valuable spatiotemporal insights into air pollution historical evolution, identifying key times and areas of heightened pollution, which can help in devising focused air quality management strategies. This research demonstrates the potential of ARIMA models in precise air quality forecasting, aiding in proactive public health initiatives and environmental policy development, consistent with Abu Dhabi’s Vision 2030.
Application of whole exome sequencing in the diagnosis of muscular disorders: a study of Taiwanese pediatric patients
Authors: Lee Chung-Lin , Chuang Chih-Kuang , Chiu Huei-Ching , Chang Ya-Hui , Tu Yuan-Rong , Lo Yun-Ting , Lin Hsiang-Yu , Lin Shuan-Pei
Published: May 2024

Background

Muscular dystrophies and congenital myopathies encompass various inherited muscular disorders that present diagnostic challenges due to clinical complexity and genetic heterogeneity.

Methods

This study aimed to investigate the use of whole exome sequencing (WES) in diagnosing muscular disorders in pediatric patients in Taiwan. Out of 161 pediatric patients suspected to have genetic/inherited myopathies, 115 received a molecular diagnosis through conventional tests, single gene testing, and gene panels. The remaining 46 patients were divided into three groups: Group 1 (multiplex ligation-dependent probe amplification–negative Duchenne muscular dystrophy) with three patients (6.5%), Group 2 (various forms of muscular dystrophies) with 21 patients (45.7%), and Group 3 (congenital myopathies) with 22 patients (47.8%).

Results

WES analysis of these groups found pathogenic variants in 100.0% (3/3), 57.1% (12/21), and 68.2% (15/22) of patients in Groups 1 to 3, respectively. WES had a diagnostic yield of 65.2% (30 patients out of 46), detecting 30 pathogenic or potentially pathogenic variants across 28 genes.

Conclusion

WES enables the diagnosis of rare diseases with symptoms and characteristics similar to congenital myopathies and muscular dystrophies, such as muscle weakness. Consequently, this approach facilitates targeted therapy implementation and appropriate genetic counseling.
Pathogenic role of different phenotypes of immune cells in airway allergic diseases: a study based on Mendelian randomization
Authors: Xu Zhihan , Li Ren , Wang Leigang , Wu Yisha , Tian Yuhe , Su Yilin , Ma Yuqiang , Li Ruiying , Wei Yao , Zhang Chen , Han Shikai , Duan Siyu , Peng Haiyi , Xue Jinmei
Published: May 2024

Background

Airway allergic disease (AAD) is a class of autoimmune diseases with predominantly Th2-type inflammation, mainly including allergic rhinitis (AR), allergic asthma (AS), and chronic sinusitis (CRS). There are very complex regulatory mechanisms between immune cells and AAD; however, previous reports found that the functions of the same immune cells in AAD are not identical.

Objective

The aim of this study was to explore the causal relationship between different phenotypic immune cells and their association with AAD.

Method

Utilizing the publicly available Genome-Wide Association Studies (GWAS) database, this study conducted a bidirectional Mendelian randomization (MR) to assess the causal relationship between immune cells of 731 different immunophenotypes and AAD. The primary assessment methods included inverse variance weighting, weighted median, and MR Egger. Additionally, sensitivity analyses such as MR-PRESSO, leave-one-out, and scatter plots were employed to eliminate the interference of heterogeneity and pleiotropy, ensuring the stability of the causal inference.

Result

A total of 38 immune cells with different immunophenotypes were found to be positively and causally associated with AR, of which 26 were protective factors and 12 were risk factors. Positive associations were found between 33 immune cells and AS, of which 14 were protective factors and 19 were risk factors, as well as between 39 immune cells and CRS, of which 22 were protective factors and 17 were risk factors. Finally, the results of all relevant immune cells for the three diseases were taken and intersected, and it was found that CD3 on CD39+-activated Treg (IVWAR = 0.001, IVWCRS = 0.043, IVWAS = 0.027) may be the key immune cell that inhibits the development of AAD (ORAR = 0.940, ORAS = 0.967, ORCRS = 0.976).

Conclusion

This study reveals that different immune phenotypes of immune cells are closely related to AAD at the genetic level, which provides a theoretical basis for future clinical studies.
Modeling the impact of floating offshore wind turbines on marine food webs in the Gulf of Lion, France
Authors: Adgé Mathieu , Lobry Jérémy , Tessier Anne , Planes Serge
Published: May 2024

To achieve its energy transition, the French government is planning to install floating wind farms in the Mediterranean Sea in the Gulf of Lion. In order to study the effects of such installations on the ecosystem, A trophic model was developed to study the evolution of biomass and ecological network indicators (ENA). Four scenarios were designed in order to simulate 1/the “reef effect” caused by the new hard substrate created by the wind farm structure, 2/the association of the reef effect with the reserve effect caused by the closure of the wind farm to fishing, 3/the impact of regular harvesting of sessile organisms from the hard substrate by fishermen and, 4/the impact of the transfer of these organisms to the seafloor. Our study suggests changes in the ecosystem structure and functioning after the introduction of a wind farm, where low trophic level groups became more important in the functioning of the trophic web, the ecosystem maturity decreased, and the overall activity and diversity increased. The biomass of some pelagic and demersal groups increased. Overall, the introduction of large wind farm platforms will transform the local ecosystem, enhancing the overall production which will likely provide benefits to local fisheries focused on higher trophic level groups.
Effects of dietary astaxanthin on growth performance, immunity, and tissue composition in largemouth bass, Micropterus salmoides
Authors: Zhang Jiankun , Yang Yeshun , Xu Hanying , Li Xinyu , Dong Fen , Chen Qiang , Han Tao , Wang Jiteng , Wu Chenglong
Published: May 2024

This study examined the effects of feeding largemouth bass (Micropterus salmoides) with diets containing different doses of astaxanthin (0, 50, 100, 150, and 200 mg/kg) for 8 weeks. The results showed that the values of weight gain significantly increased from 620.32 ± 50.38% to 826.14 ± 33.49% as dietary astaxanthin levels increased from 0 mg/kg to 100 mg/kg. When the astaxanthin level exceeded 150mg/kg, the weight gain rate showed a downward trend, but there was no significant difference among of the 100, 150 and 200 mg/kg groups. The feed conversion ratio (FCR) and protein efficiency ratio (PER) were also improved by adding astaxanthin to diets (P < 0.05). Meanwhile, adding astaxanthin to the feed increased the length and thickness of intestinal villus and muscle layer thickness (P < 0.05). The astaxanthin supplementation increased the expression of the NF-E2-related factor (Nrf2) gene and reduced malondialdehyde (MDA) content and the expression of apoptosis genes Caspase-9 and Caspase-3 (P < 0.05), indicating that it has a good antioxidant ability. Furthermore, adding astaxanthin increased the content of non-specific immune markers and decreased the expression levels of the inflammatory factors interleukin-15 (IL-15) and tumor necrosis factor-α (TNF-α). Moreover, fish fed diets with astaxanthin exhibited lower blood cortisol levels (P < 0.05). The proportions of C20:4n6 (ARA) and C20:5n3 (EPA) in the liver decreased with increasing dietary astaxanthin levels. Based on WGR and SGR values, the optimal addition level of astaxanthin in largemouth bass feed is 134.8 mg/kg ~ 135.75 mg/kg. In summary, the appropriate dietary astaxanthin enhanced the antioxidant capacity and immune response of largemouth bass and had a positive effect on its intestinal health.
Vibration analysis of piping connected with shipboard equipment
Authors: Tripathi Radharaman , Jadhav Tushar A. , Gaikwad Mahesh K. , Naidu Mithul J. , Gawand Aishwarya B. , Kaya Duran , Salunkhe Sachin , Cep Robert , Abouel Nasr Emad
Published: May 2024

The piping system connected with the shipboard equipment may be subjected to excessive vibration due to harmonic base excitation produced by hydrodynamic force imposed on the propeller blades interacting with the hull and by other sources. Vibration design aspects for shipboard pipework are often ignored, which may cause catastrophic fatigue failures and, consequently, leakage and spillage in the sea environment. Without dedicated design codes, the integrity of shipboard equipment against this environment loading can be ensured by testing as per test standard MIL-STD-167-1A (2005). However, in many cases, testing is not feasible and economically viable. Hence, this study develops an FE-based vibration analysis methodology based on MIL-STD-167-1A, which can be a valuable tool to optimize the testing requirement without compromising the integrity of these piping systems. The simulated model dynamic properties are validated with experimental modal testing and Harmonic response analysis result confirm that a mitigating solution option can be verified by a FE based vibration analysis to mitigate the vibration problem.
Case report: Hypertrophic lichen planus initially misdiagnosed as squamous cell carcinoma
Authors: Kuchimanchi Nidhi , Luu Lydia A. , Shah Preeya T. , DeSimone Jennifer
Published: May 2024

Fewer than 26 case reports describe hypertrophic lichen planus (HLP) misdiagnosed as cutaneous squamous cell carcinoma (cSCC). It can be difficult to distinguish between HLP and cSCC, as these two conditions share many clinical and histopathological characteristics. Patients who are misdiagnosed with cSCC often undergo unnecessary medical and/or surgical interventions before receiving a diagnosis of HLP and improving on HLP-directed therapy. This case series highlights the course of three female patients, referred to a single tertiary institution between 2018 and 2022, who were initially misdiagnosed with cSCC before receiving a diagnosis of HLP. We have emphasized the clinical and histopathological distinguishing features between HLP and cSCC, the pathogenesis of HLP, and current and new HLP-directed therapy. We hope that this case series serves as a reminder to dermatologists, dermatologic surgeons, and dermatopathologists to be aware of this diagnostic challenge.
Case report: Successful treatment of OXA-23 Acinetobacter baumannii neurosurgical infection and meningitis with sulbactam-durlobactam combination therapy
Authors: Snowdin Jacob W. , Mercuro Nicholas J. , Madaio Michael P. , Rawlings Stephen A.
Published: May 2024

Meningitis caused by Acinetobacter species is a rare complication of neurosurgical procedures, although it is associated with high morbidity and mortality. Carbapenem-resistant Acinetobacter is particularly difficult to treat, considering the limited selection and tolerability of effective antimicrobials. Sulbactam-durlobactam was approved by the FDA in 2023 for treatment of hospital-acquired and ventilator-associated pneumonia due to susceptible strains of Acinetobacter, including carbapenem-resistant Acinetobacter baumannii. Here, we present a case of carbapenem-resistant Acinetobacter baumannii neurosurgical infection and meningitis successfully treated with sulbactam-durlobactam combination therapy.
Motivational strategies used by health care professionals in stroke survivors in rehabilitation: a scoping review of experimental studies
Authors: Fernandes Júlio Belo , Fernandes Sónia , Domingos Josefa , Castro Cidália , Romão Ana , Graúdo Susana , Rosa Gonçalo , Franco Tânia , Ferreira Ana Patrícia , Chambino Claudine , Ferreira Bruno , Courela Susana , Ferreira Maria José , Silva Isabel , Tiago Vera , Morais Maria João , Casal Joana , Pereira Silvia , Godinho Catarina
Published: May 2024

Introduction

Cognitive and motor impairments are common among stroke survivors. Physical therapy is often used to improve the functional capacity of stroke survivors. However, limited adherence to rehabilitation programs is a challenge. Motivation plays a crucial role in the success of rehabilitation programs as it influences individual adherence to treatment and overall health outcomes. This review aims to identify current trends in motivational strategies used by healthcare professionals for stroke survivor rehabilitation.

Methods

Following the framework developed by Arksey and O’Malley, a scoping review was conducted. We performed a literature search using MEDLINE, CINAHL, the Cochrane Central Register of Controlled Trials, Nursing & Allied Health, and MedicLatina databases.

Results

A total of 906 papers were identified. After selecting and analyzing the articles, 17 papers were included in this review. Health professionals use various strategies to motivate stroke survivors. These approaches include establishing a therapeutic alliance, improving patients’ health literacy, defining realistic goals, fostering problem-solving skills, personalizing the rehabilitation program, showcasing success stories, utilizing persuasive techniques, offering encouragement and compliments, providing emotional support, and effectively managing symptoms.

Conclusion

The knowledge gathered in this review can guide healthcare professionals in helping patients overcome barriers to rehabilitation, improve their motivation, and ultimately enhance their recovery outcomes.
Effect of folA gene in human breast milk-derived Limosilactobacillus reuteri on its folate biosynthesis
Authors: Jiang Yu , Li Xianping , Zhang Wei , Ji Yadong , Yang Kai , Liu Lu , Zhang Minghui , Qiao Weicang , Zhao Junying , Du Mengjing , Fan Xiaofei , Dang Xingfen , Chen Huo , Jiang Tiemin , Chen Lijun
Published: May 2024

Introduction

Folate supplementation is crucial for the human body, and the chemically synthesized folic acid might have undesirable side effects. The use of molecular breeding methods to modify the genes related to the biosynthesis of folate by probiotics to increase folate production is currently a focus of research.

Methods

In this study, the folate-producing strain of Limosilactobacillus reuteri B1-28 was isolated from human breast milk, and the difference between B1-28 and folA gene deletion strain ΔFolA was investigated by phenotyping, in vitro probiotic evaluation, metabolism and transcriptome analysis.

Results

The results showed that the folate producted by the ΔFolA was 2–3 folds that of the B1-28. Scanning electron microscope showed that ΔFolA had rougher surface, and the acid-producing capacity (p = 0.0008) and adhesion properties (p = 0.0096) were significantly enhanced than B1-28. Transcriptomic analysis revealed that differentially expressed genes were mainly involved in three pathways, among which the biosynthesis of ribosome and aminoacyl-tRNA occurred in the key metabolic pathways. Metabolomics analysis showed that folA affected 5 metabolic pathways, involving 89 different metabolites.

Discussion

In conclusion, the editing of a key gene of folA in folate biosynthesis pathway provides a feasible pathway to improve folate biosynthesis in breast milk-derived probiotics.
Age- and sex-related changes in motor functions: a comprehensive assessment and component analysis
Authors: Wunderle Veronika , Kuzu Taylan D. , Tscherpel Caroline , Fink Gereon R. , Grefkes Christian , Weiss Peter H.
Published: May 2024

Age-related motor impairments often cause caregiver dependency or even hospitalization. However, comprehensive investigations of the different motor abilities and the changes thereof across the adult lifespan remain sparse. We, therefore, extensively assessed essential basic and complex motor functions in 444 healthy adults covering a wide age range (range 21 to 88 years). Basic motor functions, here defined as simple isolated single or repetitive movements in one direction, were assessed by means of maximum grip strength (GS) and maximum finger-tapping frequency (FTF). Complex motor functions, comprising composite sequential movements involving both proximal and distal joints/muscle groups, were evaluated with the Action Research Arm Test (ARAT), the Jebsen-Taylor Hand Function Test (JTT), and the Purdue Pegboard Test. Men achieved higher scores than women concerning GS and FTF, whereas women stacked more pins per time than men during the Purdue Pegboard Test. There was no significant sex effect regarding JTT. We observed a significant but task-specific reduction of basic and complex motor performance scores across the adult lifespan. Linear regression analyses significantly predicted the participants’ ages based on motor performance scores (R2 = 0.502). Of note, the ratio between the left- and right-hand performance remained stable across ages for all tests. Principal Component Analysis (PCA) revealed three motor components across all tests that represented dexterity, force, and speed. These components were consistently present in young (21–40 years), middle-aged (41–60 years), and older (61–88 years) adults, as well as in women and men. Based on the three motor components, K-means clustering analysis differentiated high- and low-performing participants across the adult life span. The rich motor data set of 444 healthy participants revealed age- and sex-dependent changes in essential basic and complex motor functions. Notably, the comprehensive assessment allowed for generating robust motor components across the adult lifespan. Our data may serve as a reference for future studies of healthy subjects and patients with motor deficits. Moreover, these findings emphasize the importance of comprehensively assessing different motor functions, including dexterity, force, and speed, to characterize human motor abilities and their age-related decline.
Pineal gland volume loss in females with multiple sclerosis
Authors: Vuković Miloš , Nosek Igor , Boban Jasmina , Kozić Duško
Published: May 2024

Introduction

Multiple sclerosis has a complex pathophysiology, and numerous risk factors can contribute to its development, like exposure to sunlight that is associated with serum levels of melatonin. The aim of this study was to determine whether the volume of the pineal gland, assessed by magnetic resonance imaging (MRI), correlated with the presence of multiple sclerosis.

Methods

This retrospective study included a total of 394 patients. Subjects were divided into two groups: the first group consisted of 188 patients with a definite diagnosis of multiple sclerosis (based on revised McDonald criteria) and the second group consisted of 206 healthy controls. To examine the influence of age on pineal gland volume, we stratified the whole sample into three age groups: first involved patients under 20 years, second patients between 20 and 40 years, and third group included patients over 40 years. The maximum length (L) and height (H) of the pineal gland were measured on the T1-weighted sagittal images, and the width (W) was measured on the T2-weighted coronal or axial images. The volume of the gland was calculated as an approximation to an ellipse, according to the formula V = (L × H × W)/2.

Results

Pineal gland volume of female multiple sclerosis (MS) patients (N = 129) was significantly lower than in healthy females (N = 123) (p = 0.013; p < 0.05), unlike in males where there is not such difference. Also, pineal gland volume is not age-dependent, and the observed smaller pineal gland in MS patients can reliably be attributed to the disease itself. Additionally, large pineal gland size, especially over 62.83 mm3 when compared to pineal gland volume below 31.85 mm3 is associated with more than double reduced risk of multiple sclerosis (OR 0.42; p = 0.003).

Discussion

Our results suggest that women with multiple sclerosis have smaller pineal glands that can theoretically be explained by a lack of input stimuli and the resultant decrease in gland volume. Additionally, the risk of multiple sclerosis is reduced in larger pineal gland volumes.