bluuebunny/crossref_metadata_2025 huggingface dataset:
Consolidated bioprocessing of corn cob-derived hemicellulose: engineered industrial Saccharomyces cerevisiae as efficient whole cell biocatalysts
Authors: Cunha Joana T. , Romaní Aloia , Inokuma Kentaro , Johansson Björn , Hasunuma Tomohisa , Kondo Akihiko , Domingues Lucília
Published: August 2020

Background

Consolidated bioprocessing, which combines saccharolytic and fermentative abilities in a single microorganism, is receiving increased attention to decrease environmental and economic costs in lignocellulosic biorefineries. Nevertheless, the economic viability of lignocellulosic ethanol is also dependent of an efficient utilization of the hemicellulosic fraction, which contains xylose as a major component in concentrations that can reach up to 40% of the total biomass in hardwoods and agricultural residues. This major bottleneck is mainly due to the necessity of chemical/enzymatic treatments to hydrolyze hemicellulose into fermentable sugars and to the fact that xylose is not readily consumed by Saccharomyces cerevisiae—the most used organism for large-scale ethanol production. In this work, industrial S. cerevisiae strains, presenting robust traits such as thermotolerance and improved resistance to inhibitors, were evaluated as hosts for the cell-surface display of hemicellulolytic enzymes and optimized xylose assimilation, aiming at the development of whole-cell biocatalysts for consolidated bioprocessing of corn cob-derived hemicellulose.

Results

These modifications allowed the direct production of ethanol from non-detoxified hemicellulosic liquor obtained by hydrothermal pretreatment of corn cob, reaching an ethanol titer of 11.1 g/L corresponding to a yield of 0.328 g/g of potential xylose and glucose, without the need for external hydrolytic catalysts. Also, consolidated bioprocessing of pretreated corn cob was found to be more efficient for hemicellulosic ethanol production than simultaneous saccharification and fermentation with addition of commercial hemicellulases.

Conclusions

These results show the potential of industrial S. cerevisiae strains for the design of whole-cell biocatalysts and paves the way for the development of more efficient consolidated bioprocesses for lignocellulosic biomass valorization, further decreasing environmental and economic costs.
Distribution of host-specific parasites in hybrids of phylogenetically related fish: the effects of genotype frequency and maternal ancestry?
Authors: Krasnovyd Vadym , Vetešník Lukáš , Šimková Andrea
Published: August 2020

Background

Host specificity is one of the outputs of the coevolution between parasites and their associated hosts. Several scenarios have been proposed to explain the pattern of parasite distribution in parental and hybrid genotypes ranging from hybrid resistance to hybrid susceptibility. We hypothesized that host-parasite co-adaptation limits the infection of host-specific parasites in hybrid genotypes even under the condition of the high frequency of hybrids. The experimental monogenean infection in pure breeds of Blicca bjoerkna and Abramis brama and cross-breeds (the F1 generation of hybrids) under the condition of similar frequencies of pure and hybrid genotypes was investigated. We also examined the potential effect of the maternal origin of hybrids (potential co-adaptation at the level of mitochondrial genes) on monogenean abundance.

Methods

Pure breeds of two cyprinids and two cross-breeds (one with B. bjoerkna, the next with A. brama in the maternal positions) were exposed to infection by monogeneans naturally occurring in B. bjoerkna and A. brama. The experiment was run under similar frequencies of the four breed lines.

Results

We showed similar levels of monogenean infection in B. bjoerkna and A. brama. However, each species harboured specific monogenean fauna. Hybrids harboured all monogenean species specifically infecting one or the other species. Monogenean infection levels, especially those of Dactylogyrus specific to A. brama, were lower in hybrids. For the majority of host-specific parasites, there was no effect of the maternal origin of hybrids on monogenean abundance. Asymmetry was found in the distribution of specific parasites in favour of specialists of B. bjoerkna in the monogenean communities of hybrids.

Conclusions

Our results indicate that the maternal mtDNA of hybrids is not an important predictor of host-specific monogenean infection, which may suggest that mitochondrial genes are not strongly involved in the coadaptation between monogeneans and their associated hosts. The asymmetry of species-specific parasites suggests similarity between the molecular components of the immune mechanisms in hybrids and B. bjoerkna. Our results revealed a difference between the degree of host-parasite coadaptation in specific parasites of A. brama and the degree of host-parasite coadaptation in specific parasites of B. bjoerkna and their associated hosts.
Low B cell counts as risk factor for infectious complications in systemic sclerosis after autologous hematopoietic stem cell transplantation
Authors: Gernert Michael , Tony Hans-Peter , Schwaneck Eva Christina , Fröhlich Matthias , Schmalzing Marc
Published: August 2020

Background

Autologous hematopoietic stem cell transplantation (aHSCT) is a treatment option for a selected group of systemic sclerosis (SSc) patients with good available evidence but can be associated with considerable morbidity and mortality. The aim of this study was to describe infectious complications and distinct immune reconstitution patterns after aHSCT and to detect risk factors in lymphocyte subsets, which are associated with an elevated rate of infections after aHSCT.

Methods

Seventeen patients with SSc were included in this single-center retrospective cohort study. Clinical and laboratory data was collected before and for 12 months after aHSCT, including immunophenotyping of peripheral whole blood by fluorescence-activated cell sorting.

Results

Cytomegalovirus (CMV) reactivations were common in CMV-IgG-positive patients (50%) and needed treatment. Mycotic infections occurred in 17.6%. One patient died (resulting in a mortality of 5.9%) due to pneumonia with consecutive sepsis. All patients showed decreased T helper cells (CD3+/CD4+) and within the B cell compartment decreased post-switched memory B cells (CD19+/CD27+/IgD−) and elevated naïve B cells (CD19+/CD27−/IgD+) until 12 months after aHSCT. Patients who developed infections had significantly lower B cells before aHSCT than patients who did not develop infections.

Conclusion

After aHSCT, monitoring for infectious complications, especially for CMV reactivations, is crucial as the reconstitution of the immune system takes longer than 12 months. Low peripheral B cells might be a risk factor for an elevated infection rate.
IL13Rα1 protects against rheumatoid arthritis by combating the apoptotic resistance of fibroblast-like synoviocytes
Authors: Yang Xiaomei , Guo Qingwei , Feng Tingting , Lu Qiqi , Ge Luna , Pan Jihong , Bi Kehong , Qiao Li , Tian Lei , Xie Tianhua , Yao Chengfang , Song Guanhua , Wang Lin
Published: August 2020

Background

Endoplasmic reticulum (ER) stress is closely related with the pathological progression of rheumatoid arthritis (RA), and fibroblast-like synoviocytes (FLSs) are known as its resistance against ER stress-induced apoptosis. Studies on overcoming such resistance would provide a novel treatment strategy for RA in a clinical setting.

Methods

IL13Rα1 expression was assessed in the synovial tissue by RT-qPCR, immunohistology, and Western blot. Gain or loss of functional analysis was applied to evaluate the biological roles of IL13Rα1 in RA FLSs. Cell viability and apoptosis were assessed by MTS, Western blot, and flow cytometry. The therapeutic effects of IL13Rα1 on the severity of type II collagen-induced arthritis (CIA) in DBA-/1 mouse model were evaluated by scoring synovitis, hyperplasia, cartilage degradation, and bone destruction.

Results

IL13Rα1 expression was selectively downregulated when RA FLSs were stimulated by ER stress inducers. Functionally, IL13Rα1 overexpression could inhibit the viability, but induce the apoptosis of RA FLSs in the presence of ER stress inducers. Mechanistically, IL13Rα1 promotes cell apoptosis via transcriptionally activating trail expression. Besides, IL13Rα1 could interact and stabilize DR5 protein, thus forming a positive loop involving trail and DR5 to render RA FLSs more susceptible to apoptosis. Additionally, intraarticular injection of IL13Rα1 conferred therapeutic effects in CIA models and showed a limited degree of synovial proliferation and joint destruction.

Conclusions

Together, our data establishes a regulatory role for IL13Rα1 to combat the apoptotic resistance of RA FLSs against ER stress. The inhibitory effects of IL13Rα1 on arthritis progression suggest the therapeutic potential in RA.
Meta-analysis of genome-wide association study identifies FBN2 as a novel locus associated with systemic lupus erythematosus in Thai population
Authors: Tangtanatakul Pattarin , Thumarat Chisanu , Satproedprai Nusara , Kunhapan Punna , Chaiyasung Tassamonwan , Klinchanhom Siriwan , Wang Yong-Fei , Wei Wei , Wongshinsri Jeerapat , Chiewchengchol Direkrit , Rodsaward Pongsawat , Ngamjanyaporn Pintip , Suangtamai Thanitta , Mahasirimongkol Surakameth , Pisitkun Prapaporn , Hirankarn Nattiya
Published: August 2020

Background

Differences in the expression of variants across ethnic groups in the systemic lupus erythematosus (SLE) patients have been well documented. However, the genetic architecture in the Thai population has not been thoroughly examined. In this study, we carried out genome-wide association study (GWAS) in the Thai population.

Methods

Two GWAS cohorts were independently collected and genotyped: discovery dataset (487 SLE cases and 1606 healthy controls) and replication dataset (405 SLE cases and 1590 unrelated disease controls). Data were imputed to the density of the 1000 Genomes Project Phase 3. Association studies were performed based on different genetic models, and pathway enrichment analysis was further examined. In addition, the performance of disease risk estimation for individuals in Thai GWAS was assessed based on the polygenic risk score (PRS) model trained by other Asian populations.

Results

Previous findings on SLE susceptible alleles were well replicated in the two GWAS. The SNPs on HLA class II (rs9270970, A>G, OR = 1.82, p value = 3.61E−26), STAT4 (rs7582694, C>G, OR = 1.57, p value = 8.21E−16), GTF2I (rs73366469, A>G, OR = 1.73, p value = 2.42E−11), and FAM167A-BLK allele (rs13277113, A>G, OR = 0.68, p value = 1.58E−09) were significantly associated with SLE in Thai population. Meta-analysis of the two GWAS identified a novel locus at the FBN2 that was specifically associated with SLE in the Thai population (rs74989671, A>G, OR = 1.54, p value = 1.61E−08). Functional analysis showed that rs74989671 resided in a peak of H3K36me3 derived from CD14+ monocytes and H3K4me1 from T lymphocytes. In addition, we showed that the PRS model trained from the Chinese population could be applied in individuals of Thai ancestry, with the area under the receiver-operator curve (AUC) achieving 0.76 for this predictor.

Conclusions

We demonstrated the genetic architecture of SLE in the Thai population and identified a novel locus associated with SLE. Also, our study suggested a potential use of the PRS model from the Chinese population to estimate the disease risk for individuals of Thai ancestry.
Intestinal volvulus in the pump twin of a twin reversed arterial perfusion (TRAP) sequence after laser therapy at 18 weeks: a case report
Authors: Herrera Tania T. , Rueda Katia , Espinosa Honorina , Britton Gabrielle B.
Published: August 2020

Background

Twin reversed arterial perfusion sequence is a rare and potentially lethal condition affecting approximately 1% of monochorionic twin pregnancies and 1 in 35,000 pregnancies overall. An apparently normal (pump) twin perfuses its severely malformed cotwin with deoxygenated blood via retrograde flow in direct arterioarterial anastomoses between the umbilical arteries of each twin. Fetal intestinal volvulus is a rare condition usually manifesting after birth. We report a unique case of twin reversed arterial perfusion sequence in association with intestinal volvulus in the surviving pump twin.

Case presentation

A 32-year-old Hispanic primigravida was referred to our clinic after a fetoscopy procedure of laser photocoagulation of anastomoses at 18 weeks of gestation. Follow up scans in the ex-pump twin revealed dilated bowel loops and a typical “whirlpool sign” at 26 weeks of gestation, and intrauterine intestinal volvulus was suspected. At 29 weeks of gestation, preterm premature rupture of membranes occurred, and an emergency cesarean section was performed. The newborn was diagnosed in the early neonatal period with intestinal perforation. The diagnosis was postnatally confirmed by surgery and histopathology.

Conclusions

The type of fetal intervention and late gestational age of the procedure increase the risk of complications. This case alerts health providers to be vigilant in the follow-up of patients with complicated monochorionic pregnancies.
Rat epidermal stem cells promote the angiogenesis of full-thickness wounds
Authors: Huang Shaobin , Hu Zhicheng , Wang Peng , Zhang Yi , Cao Xiaoling , Dong Yunxian , Cheng Pu , Xu Hailin , Zhu Wenkai , Tang Bing , Zhu Jiayuan
Published: August 2020

Background

Full-thickness wounds severely affect patients’ life quality and become challenging problems for clinicians. Stem cells have great prospects in the treatment of wounds. Our previous study confirmed that autologous basal cell suspension could promote wound healing, and epidermal stem cells (ESCs) were detected in the basal cell suspension. Herein, this study aimed to explore the effect of ESCs on full-thickness wounds.

Methods

Rat ESCs were isolated and expanded and then were transfected with lentivirus to stably express enhanced green fluorescent protein. The experimental rats were randomly divided into 2 groups: in the ESC group, the rat ESCs were sprayed on the full-thickness wounds of rats; in the control group, phosphate-buffered saline was sprayed the on the wounds. Next, wound healing and neovascularization were evaluated. Colonization, division, and differentiation of ESCs on the wound were analyzed by immunofluorescence.

Results

The rat ESCs colonized, divided, and proliferated in the wound. Additionally, rat ESCs around blood vessels differentiated into vascular endothelial cells and formed a lumen-like structure. Compared with the control group, the ESC group showed enhanced angiogenesis and accelerated wound healing.

Conclusions

Our study confirmed that rat ESCs are safe and effective for treating full-thickness wounds. Additionally, under certain conditions, ESCs can differentiate into vascular endothelial cells to promote angiogenesis and wound healing.
Biological characteristics of endometriotic mesenchymal stem cells isolated from ectopic lesions of patients with endometriosis
Authors: Liu Yanli , Liang Shengying , Yang Fen , Sun Yuliang , Niu Lidan , Ren Yakun , Wang Hongmei , He Yanan , Du Jiang , Yang Jun , Lin Juntang
Published: August 2020

Background

Research into the pathogenesis of endometriosis (EMs) would substantially promote its effective treatment and early diagnosis. However, the aetiology of EMs is poorly understood and controversial despite the progress in EMs research in the last several decades. Currently, accumulating evidence has shed light on the importance of endometrial stem cells (EnSCs) residing in the basal layer of endometrium in the establishment and progression of endometriotic lesions. Therefore, we aimed to identify the differences between EnSCs isolated from the ectopic lesions of EMs patients (EnSC-EM-EC) and EnSCs isolated from eutopic endometrium of control group (EnSC-Control). We further performed preliminary exploration of the potential signalling pathways involved in the above abnormalities.

Methods

EnSC-EM-EC (n = 12) and EnSC-Control (n = 13) were successfully isolated. Then, the proliferative capacity, migratory capacity and angiogenic potential of EnSCs were evaluated by conventional MTT assay, flow cytometry, wound healing assay, transwell assay, tube formation assay and chick embryo chorioallantoic membrane assay respectively. The expression of 11 angiogenesis-associated biological factors and 11 cytokines secreted by EnSCs and 17 adhesion molecules expressed on EnSCs were determined by protein array assays respectively. Differentially expressed genes (DEGs) between EnSC-EM-EC and EnSC-Control were analysed by RNA-sequence.

Results

EnSC-EM-EC exhibited unique biological characteristics, including prolonged mitosis, enhanced migratory capacity and enhanced angiogenic potential. Greater amounts of angiogenic factors (especially VEGF and PDGF) were secreted by EnSC-EM-EC than by EnSC-Control; however, the distinct profiles of cytokines secreted by EnSC-EM-EC and adhesion molecules expressed by EnSC-EM-EC require further investigation. A total of 523 DEGs between EnSC-EM-EC and EnSC-Control were identified and analysed using the KEGG and Gene Ontology databases.

Conclusions

Our results not only improve the understanding of EMs but also contribute to the development of EnSC-EM-EC as a tool for EMs drug discovery. These cells could be of great help in exploiting promising therapeutic targets and new biomarkers for EMs treatment and prognosis.
Dopamine transporter neuroimaging accurately assesses the maturation of dopamine neurons in a preclinical model of Parkinson’s disease
Authors: Goggi Julian L. , Qiu Lifeng , Liao Mei Chih , Khanapur Shivashankar , Jiang Lingfan , Boominathan Ramasamy , Hartimath Siddesh V. , Cheng Peter , Yong Fui Fong , Soh Vanessa , Deng Xiaozhou , Lin Youshan Melissa , Haslop Anna , Tan Peng Wen , Zeng Xiaoxia , Lee Jolene W. L. , Zhang Zhiwei , Sadasivam Pragalath , Tan Eng King , Luthra Sajinder K. , Shingleton William D. , Oh Steve K. W. , Zeng Li , Robins Edward G.
Published: August 2020

Background

Significant developments in stem cell therapy for Parkinson’s disease (PD) have already been achieved; however, methods for reliable assessment of dopamine neuron maturation in vivo are lacking. Establishing the efficacy of new cellular therapies using non-invasive methodologies will be critical for future regulatory approval and application. The current study examines the utility of neuroimaging to characterise the in vivo maturation, innervation and functional dopamine release of transplanted human embryonic stem cell-derived midbrain dopaminergic neurons (hESC-mDAs) in a preclinical model of PD.

Methods

Female NIH RNu rats received a unilateral stereotaxic injection of 6-OHDA into the left medial forebrain bundle to create the PD lesion. hESC-mDA cell and sham transplantations were carried out 1 month post-lesion, with treated animals receiving approximately 4 × 105 cells per transplantation. Behavioural analysis, [18F]FBCTT and [18F]fallypride microPET/CT, was conducted at 1, 3 and 6 months post-transplantation and compared with histological characterisation at 6 months.

Results

PET imaging revealed transplant survival and maturation into functional dopaminergic neurons. [18F]FBCTT-PET/CT dopamine transporter (DAT) imaging demonstrated pre-synaptic restoration and [18F]fallypride-PET/CT indicated functional dopamine release, whilst amphetamine-induced rotation showed significant behavioural recovery. Moreover, histology revealed that the grafted cells matured differently in vivo producing high- and low-tyrosine hydroxylase (TH) expressing cohorts, and only [18F]FBCTT uptake was well correlated with differentiation.

Conclusions

This study provides further evidence for the value of in vivo functional imaging for the assessment of cell therapies and highlights the utility of DAT imaging for the determination of early post-transplant cell maturation and differentiation of hESC-mDAs.
NF-κB signaling in rheumatoid arthritis with focus on fibroblast-like synoviocytes
Authors: Nejatbakhsh Samimi Leila , Farhadi Elham , Tahmasebi Mohammad Naghi , Jamshidi Ahmadreza , Sharafat Vaziri Arash , Mahmoudi Mahdi
Published: August 2020

The nuclear factor-κB (NF-κB) signaling pathway regulates multiple processes in innate and adaptive immune cells. This pathway is involved in inflammation through the regulation of cytokines, chemokines, and adhesion molecules expression. The NF-κB transcription factor also participates in the survival, proliferation, and differentiation of cells. Therefore, deregulated NF-κB activation contributes to the pathogenesis of inflammatory diseases. Rheumatoid arthritis (RA) is classified as a heterogeneous and complex autoimmune inflammatory disease. Although different immune and non-immune cells contribute to the RA pathogenesis, fibroblast-like synoviocytes (FLSs) play a crucial role in disease progression. These cells are altered during the disease and produce inflammatory mediators, including inflammatory cytokines and matrix metalloproteinases, which result in joint and cartilage erosion. Among different cell signaling pathways, it seems that deregulated NF-κB activation is associated with the inflammatory picture of RA. NF-κB activation can also promote the proliferation of RA-FLSs as well as the inhibition of FLS apoptosis that results in hyperplasia in RA synovium. In this review, the role of NF-κB transcription factor in immune and non-immune cells (especially FLSs) that are involved in RA pathogenesis are discussed.
An age and space structured SIR model describing the Covid-19 pandemic
Authors: Colombo Rinaldo M. , Garavello Mauro , Marcellini Francesca , Rossi Elena
Published: August 2020

We present an epidemic model capable of describing key features of the Covid-19 pandemic. While capturing several qualitative properties of the virus spreading, it allows to compute the basic reproduction number, the number of deaths due to the virus and various other statistics. Numerical integrations are used to illustrate the adherence of the evolutions described by the model to specific well known real features of the present pandemic. In particular, this model is consistent with the well known relevance of quarantine, shows the dramatic role of care houses and accounts for the increase in the death toll when spatial movements are not constrained.
Surface display of p75, a Lactobacillus rhamnosus GG derived protein, on Bacillus subtilis spores and its antibacterial activity against Listeria monocytogenes
Authors: Kang Soo Ji , Jun Ji Su , Moon Jeong A , Hong Kwang Won
Published: August 2020

Lactobacillus rhamnosus p75 protein with peptidoglycan hydrolase (PGH) activity is one of the key molecules exhibiting anti-apoptotic and cell-protective activity for human intestinal epithelial cells. In this study, with the goal of developing new probiotics, the p75 protein was displayed on the surface of Bacillus subtilis spores using spore coat protein CotG as an anchoring motif. The PGH activity, stability, and the antibacterial activity of the spore-displayed p75 (CotG-p75) protein were also investigated. The PGH activity of the CotG-p75 against peptidoglycan extracted from B. subtilis was confirmed by the ninhydrin test. Under various harsh conditions, compared to the control groups, the PGH activities of CotG-p75 were very stable in the range of pH 3–7 and maintained at 70% at 50 °C. In addition, the antibacterial activity of CotG-p75 against Listeria monocytogenes was evaluated by a time-kill assay. After 6 h incubation in phosphate-buffered saline, CotG-p75 reduced the number of viable cells of L. monocytogenes by up to 2.0 log. Scanning electron microscopy analysis showed that the cell wall of L. monocytogenes was partially damaged by the treatment with CotG-p75. Our preliminary results show that CotG-p75 could be a good candidate for further research to develop new genetically engineered probiotics.
Use of powered air-purifying respirator (PAPR) by healthcare workers for preventing highly infectious viral diseases—a systematic review of evidence
Authors: Licina Ana , Silvers Andrew , Stuart Rhonda L.
Published: August 2020

Background

Healthcare workers (HCWs) are at particular risk during pandemics and epidemics of highly virulent diseases with significant morbidity and case fatality rate. These diseases include severe acute respiratory syndrome coronaviruses, SARS-CoV-1 and SARS-CoV-2, Middle Eastern Respiratory Syndrome (MERS), and Ebola. With the current (SARS-CoV-2) global pandemic, it is critical to delineate appropriate contextual respiratory protection for HCWs. The aim of this systematic review was to evaluate the effect of powered air-purifying respirators (PAPRs) as part of respiratory protection versus another device (egN95/FFP2) on HCW infection rates and contamination.

Methods

Our primary outcomes included HCW infection rates with SARS-CoV-2, SARS-CoV-1, Ebola, or MERS when utilizing PAPR. We included randomized controlled trials, non-randomized controlled trials, and observational studies. We searched the following databases: MEDLINE, EMBASE, and Cochrane Library (Cochrane Database of Systematic Reviews and CENTRAL). Two reviewers independently screened all citations, full-text articles, and abstracted data. Due to clinical and methodological heterogeneity, we did not conduct a meta-analysis. Where applicable, we constructed evidence profile (EP) tables for each individual outcome. Confidence in cumulative evidence for each outcome was classified according to the GRADE system.

Results

We identified 689 studies during literature searches. We included 10 full-text studies. A narrative synthesis was provided. Two on-field studies reported no difference in the rates of healthcare workers performing airway procedures during the care of critical patients with SARS-CoV-2. A single simulation trial reported a lower level of cross-contamination of participants using PAPR compared to alternative respiratory protection. There is moderate quality evidence that PAPR use is associated with greater heat tolerance but lower scores for mobility and communication ability. We identified a trend towards greater self-reported wearer comfort with PAPR technology in low-quality observational simulation studies.

Conclusion

Field observational studies do not indicate a difference in healthcare worker infection utilizing PAPR devices versus other compliant respiratory equipment. Greater heat tolerance accompanied by lower scores of mobility and audibility in PAPR was identified. Further pragmatic studies are needed in order to delineate actual effectiveness and provider satisfaction with PAPR technology.

Systematic review registration

The protocol for this review was prospectively registered with the International Register of Systematic Reviews identification numberCRD42020184724.
A generalized Hölder-type inequalities for measurable operators
Authors: Han Yazhou , Shao Jingjing
Published: August 2020

We prove a generalized Hölder-type inequality for measurable operators associated with a semi-finite von Neumann algebra which is a generalization of the result shown by Bekjan (Positivity 21:113–126, 2017). This also provides a generalization of the unitarily invariant norm inequalities for matrix due to Bhatia–Kittaneh, Horn–Mathisa, Horn–Zhan and Zou under a cohyponormal condition.
The convergence rate of truncated hypersingular integrals generated by the modified Poisson semigroup
Authors: Eryiğit Melih , Evcan Sinem Sezer , Çobanoğlu Selim
Published: August 2020

Hypersingular integrals have appeared as effective tools for inversion of multidimensional potential-type operators such as Riesz, Bessel, Flett, parabolic potentials, etc. They represent (at least formally) fractional powers of suitable differential operators. In this paper the family of the so-called “truncated hypersingular integral operators” $\mathbf{D}_{\varepsilon }^{\alpha }f$

D
ε
α

f
is introduced, that is generated by the modified Poisson semigroup and associated with the Flett potentials

F
α

φ
=

(
E
+

−
Δ

)

−
α

φ
($0<\alpha <\infty $
0
<
α
<
∞
, $\varphi \in L_{p}(\mathbb{R}^{n})$
φ
∈

L
p

(

R
n

)
). Then the relationship between the order of “$L_{p}$

L
p

-smoothness” of a function f and the “rate of $L_{p}$

L
p

-convergence” of the families $\mathbf{D}_{\varepsilon }^{\alpha } \mathcal{F}^{\alpha }f$

D
ε
α

F
α

f
to the function f as $\varepsilon \rightarrow 0^{+}$
ε
→

0
+

is also obtained.
Commutators of θ-type generalized fractional integrals on non-homogeneous spaces
Authors: Lu Guanghui
Published: August 2020

The aim of this paper is to establish the boundednes of the commutator $[b,T_{\alpha }]$
[
b
,

T
α

]
generated by θ-type generalized fractional integral $T_{\alpha }$

T
α

and $b\in \widetilde{\mathrm{RBMO}}(\mu )$
b
∈

RBMO
˜

(
μ
)
over a non-homogeneous metric measure space. Under the assumption that the dominating function λ satisfies the ϵ-weak reverse doubling condition, the author proves that the commutator $[b,T_{\alpha }]$
[
b
,

T
α

]
is bounded from the Lebesgue space $L^{p}(\mu )$

L
p

(
μ
)
into the space $L^{q}(\mu )$

L
q

(
μ
)
for $\frac{1}{q}=\frac{1}{p}-\alpha $

1
q

=

1
p

−
α
and $\alpha \in (0,1)$
α
∈
(
0
,
1
)
, and bounded from the atomic Hardy space $\widetilde{H}^{1}_{b}(\mu )$

H
˜

b
1

(
μ
)
with discrete coefficient into the space $L^{\frac{1}{1-\alpha },\infty }(\mu )$

L

1

1
−
α

,
∞

(
μ
)
. Furthermore, the boundedness of the commutator $[b,T_{\alpha }]$
[
b
,

T
α

]
on a generalized Morrey space and a Morrey space is also got.
Extragradient-like method for pseudomontone equilibrium problems on Hadamard manifolds
Authors: Chen Junfeng , Liu Sanyang
Published: August 2020

This paper presents an extragradient-like method for solving a pseudomonotone equilibrium problem with a Lipschitz-type condition on Hadamard manifolds. The algorithm only needs to know the existence of the Lipschitz-type constants of the bifunction, and the stepsize of each iteration is determined by the adjacent iterations. Convergence of the algorithm is analyzed, and its application to variational inequalities is also provided. Finally, several experiments are made to verify the effectiveness of the algorithms.
Stakeholders’ satisfaction as a key determinant of critical success factors in renewable energy projects
Authors: Maqbool Rashid , Deng Xiaomei , Rashid Yahya
Published: August 2020

Renewable energy projects (REPs) are critical to providing a clean and sustainable environmental perspective alongside the economic prosperity of any country. Unfortunately, recent trends in renewable energy projects (REPs) are not positive enough regarding their successful completion within the budgeted cost, planned time, proposed quality, and other necessary constraints. Although the pertinent literature has discussed the critical success factors (CSFs) for such projects, their influencing mechanism with respect to the role of key stakeholders is still overlooked. This study recognizes several critical success factors (CSFs) and their influencing mechanisms onto renewable energy projects (REPs) in order to evaluate direct influences onto project success as well as indirect influences through stakeholders’ satisfaction (SS) as mediation on the project success of small and medium-sized renewable energy projects (REPs) in Pakistan’s energy sector. A sample of about 272 respondents working with renewable energy projects (REPs), including key stakeholders, has been collected to perform data analysis and draw inferences. A structural equation modeling (SEM) approach was used for data analysis and inference drawing. The results show that though there is a positive and direct association between critical success factors (CSFs) and project success, a partial mediating role of stakeholders’ satisfaction (SS) between CSFs and project success was still determined. Hence, it was found that CSFs significantly contribute to renewable energy projects (REPs) through mediating influences of stakeholders’ satisfaction (SS). The findings confirmed that stakeholders’ satisfaction (SS) is a key determinant for critical success factors (CSFs) in renewable energy projects (REPs). Moreover, stakeholders’ satisfaction (SS) emerged to be a crucial element for the completion of such projects. The empirical findings of this study might have a useful effect on academicians and the practitioners engaged with renewable energy projects (REPs).
High rate of extended-spectrum beta-lactamase-producing gram-negative infections and associated mortality in Ethiopia: a systematic review and meta-analysis
Authors: Tufa Tafese B. , Fuchs Andre , Tufa Takele B. , Stötter Loraine , Kaasch Achim J. , Feldt Torsten , Häussinger Dieter , Mackenzie Colin R.
Published: August 2020

Background

Extended-spectrum beta-lactamase (ESBL)-producing Gram-negative bacteria have become a serious threat to global health. Their rapid spread is associated with high mortality due to ineffective antibiotic treatment. To date a regular surveillance of multidrug-resistant (MDR) pathogens in Ethiopia is not established. For this report, published data regarding ESBL-producing bacteria in different health facilities of Ethiopia were reviewed.

Methods

This study collates data from published information on the rates and clinical implications of infection with ESBL-producing Gram-negative bacteria in Ethiopia. A systematic literature search was conducted using PubMed, PubMed Central, Medline, Science Direct and Google scholar from October 2018 to March 2019. Eligible studies were identified by applying quality criteria. The pooled proportion of ESBL-producing Gram-negative bacteria was estimated based on a random effect model. The publication bias and the variation in proportion estimates attributed to heterogeneity were assessed.

Results

Fourteen studies with relevant data were included in the review. In total, 1649 Gram-negative bacteria isolated from 5191 clinical samples were included. The pooled proportion estimate of ESBL-producing Gram-negative bacteria was 50% (95% CI: 47.7–52.5%. Data showed a high level of heterogeneity (I2 = 95%, P <  0.01). ESBL rates varied by species; 65.7% (263/400) in Klebsiella spp., 48.4% (90/186) in Salmonella spp., and 47.0% (383/815) in E. coli. ESBL-encoding genes were reported in 81 isolates: 67 isolates harbored the CTX-M-1 group and 14 isolates TEM. The mortality associated with infections by bacteria resistant to third generation cephalosporins has rarely been investigated. However, two studies reported a mortality of 33.3% (1/3) and 100% (11/11).

Conclusions

In this meta-analysis, the pooled prevalence of ESBL-producing pathogens is alarmingly high. Data on mortality rates is scarce. This highlights the need for establishing and upgrading clinical microbiology laboratories in Ethiopia for routine antibiotic susceptibility testing and extended surveillance of multidrug resistance.
The effect of eye movement desensitization and reprocessing on the fear of hypoglycemia in type 2 diabetic patients: a randomized clinical trial
Authors: Sheikhi Mohammadreza , Moradi Mohamad , Shahsavary Saeed , Alimoradi Zainab , Salimi Hamid Reza
Published: August 2020

Background

The fear of hypoglycemia leads to psychological symptoms in patients with diabetes type 2. In this research, the effects of EDMR on the fear of hypoglycemia in patients with diabetes type 2 were examined.

Methods

A clinical trial study was carried out with participation of 72 patients who had diabetes type 2 in Velayat Hospital. The participants were randomly assigned into control and intervention groups. The intervention group received EMDR. The required information was gleaned using a questionnaire of fear of hypoglycemia, intensity of hypoglycemia, and demographics filled out before the intervention, and 1 month and 3 months after it. The data were analyzed using descriptive statistics on SPSS Version 23. For comparison of fear of hypoglycemia in intervention and control groups, repeated measure ANOVA and Cohen d test were used.

Results

The mean age of the participants in the intervention group was 43.17 ± 10.55 and in the control group was 45.86 ± 13.6. In this study, without considering the potential disruptors in the incorrect model, the intervention caused a reduction of 15 points 1 month after the completion of the intervention and a reduction of 17 points 3 month after the completion of the intervention on the scale of fear of hypoglycemia; but post-correction of potential disruptors, intervention caused a reduction of 19.5 scores 1 month after the completion and a reduction of 20.3 scores 3 months after the intervention .

Conclusions

The EMDR can be used as a non-pharmaceutical treatment method to treat and alleviate the fear of hypoglycemia in type 2 diabetes patients.

Trial registration

Iranian Registry of Clinical Trials: IRCT20181201041813N1, 2019/11/13.
Clinical, radiological and molecular characterization of intramedullary astrocytomas
Authors: Lebrun Laetitia , Meléndez Barbara , Blanchard Oriane , De Nève Nancy , Van Campenhout Claude , Lelotte Julie , Balériaux Danielle , Riva Matteo , Brotchi Jacques , Bruneau Michaël , De Witte Olivier , Decaestecker Christine , D’Haene Nicky , Salmon Isabelle
Published: August 2020

Intramedullary astrocytomas (IMAs) are rare tumors, and few studies specific to the molecular alterations of IMAs have been performed. Recently, KIAA1549-BRAF fusions and the H3F3A p.K27M mutation have been described in low-grade (LG) and high-grade (HG) IMAs, respectively. In the present study, we collected clinico-radiological data and performed targeted next-generation sequencing for 61 IMAs (26 grade I pilocytic, 17 grade II diffuse, 3 LG, 3 grade III and 12 grade IV) to identify KIAA1549-BRAF fusions and mutations in 33 genes commonly implicated in gliomas and the 1p/19q regions. One hundred seventeen brain astrocytomas were analyzed for comparison. While we did not observe a difference in clinico-radiological features between LG and HG IMAs, we observed significantly different overall survival (OS) and event-free survival (EFS). Multivariate analysis showed that the tumor grade was associated with better OS while EFS was strongly impacted by tumor grade and surgery, with higher rates of disease progression in cases in which only biopsy could be performed. For LG IMAs, EFS was only impacted by surgery and not by grade. The most common mutations found in IMAs involved TP53, H3F3A p.K27M and ATRX. As in the brain, grade I pilocytic IMAs frequently harbored KIAA1549-BRAF fusions but with different fusion types. Non-canonical IDH mutations were observed in only 2 grade II diffuse IMAs. No EGFR or TERT promoter alterations were found in IDH wild-type grade II diffuse IMAs. These latter tumors seem to have a good prognosis, and only 2 cases underwent anaplastic evolution. All of the HG IMAs presented at least one molecular alteration, with the most frequent one being the H3F3A p.K27M mutation. The H3F3A p.K27M mutation showed significant associations with OS and EFS after multivariate analysis. This study emphasizes that IMAs have distinct clinico-radiological, natural evolution and molecular landscapes from brain astrocytomas.
Microglia depletion diminishes key elements of the leukotriene pathway in the brain of Alzheimer’s Disease mice
Authors: Michael J. , Unger M. S. , Poupardin R. , Schernthaner P. , Mrowetz H. , Attems J. , Aigner L.
Published: August 2020

Leukotrienes (LTs) contribute to the neuropathology of chronic neurodegenerative disorders including Alzheimer’s Disease (AD), where they mediate neuroinflammation and neuronal cell-death. In consequence, blocking the action of Leukotrienes (LTs) ameliorates pathologies and improves cognitive function in animal models of neurodegeneration. Surprisingly, the source of Leukotrienes (LTs) in the brain is largely unknown. Here, we identified the Leukotriene (LT) synthesis rate-limiting enzyme 5-Lipoxygenase (5-Lox) primarily in neurons and to a lesser extent in a subpopulation of microglia in human Alzheimer´s Disease (AD) hippocampus brain sections and in brains of APP Swedish PS1 dE9 (APP-PS1) mice, a transgenic model for Alzheimer´s Disease (AD) pathology. The 5-Lipoxygenase (5-Lox) activating protein (FLAP), which anchors 5-Lipoxygenase (5-Lox) to the membrane and mediates the contact to the substrate arachidonic acid, was confined exclusively to microglia with the entire microglia population expressing 5-Lipoxygenase activating protein (FLAP). To define the contribution of microglia in the Leukotriene (LT) biosynthesis pathway, we ablated microglia using the colony stimulating factor 1 receptor (CSF1R) inhibitor PLX5622 in wildtype (WT) and APP-PS1 mice. Microglia ablation not only diminished the expression of FLAP and of the Leukotriene (LT) receptor Cysteinylleukotriene receptor 1 (CysLTR1), as expected based on their microglia cell type-specific expression, but also drastically reduced 5-Lipoxygenase (5-Lox) mRNA expression in the brain and its protein expression in neurons, in particular in wildtype (WT) mice. In conclusion i) microglia are key in Leukotriene (LT) biosynthesis, and ii) they regulate neuronal 5-Lipoxygenase (5-Lox) expression implying a yet unknown signaling mechanism between neurons and microglia.
Co-expression of NMDA-receptor subunits NR1, NR2A, and NR2B in dysplastic neurons of teratomas in patients with paraneoplastic NMDA-receptor-encephalitis: a retrospective clinico-pathology study of 159 patients
Authors: Jiang Xin-Yue , Lei Song , Zhang Le , Liu Xu , Lin Min-Tao , Blumcke Ingmar , Piao Yue-Shan , Zhou Dong , Li Jin-Mei
Published: August 2020

Objective

To comprehensively describe the pathological features of neurons in patients with ovarian teratomas and paraneoplastic anti-NMDAR encephalitis (anti-NMDARE), emphasizing on NMDA-receptor expression and infiltrating lymphocytes.

Methods

A retrospective study was performed in a large series of 159 patients from the West China Hospital. We retrospectively identified 12 patients with paraneoplastic anti-NMDARE (11 case with ovarian teratomas and 1 case with mixed germ cell tumor), which were compared to 35 patients with teratomas and no encephalitis and to 147 patients with anti-NMDARE and no evidence for tumors. Patient history and outcome were reviewed from the clinical charts and compared between all three groups. Histopathological examination, including double-immunofluorescence of NMDAR subunits and IgG was performed in all teratoma tissues. Magnetic Luminex Assay Human Premixed Multi-Analyte Kit was performed to investigate cytokines profile of CSF.

Results

Patients with paraneoplastic anti-NMDARE had a more severe clinical presentation, i.e. they required more mechanical ventilation and intensive care (p < 0.001). Though immunotherapy was initiated earlier in this group, repeated intravenous immunoglobulin administration (IVIG) was more common (p = 0.002) and with higher cerebrospinal fluid (CSF) antibody titres (p = 0.004). Following tumor resection, the outcome did not differ between groups. A peculiar population of floating-frog like dysplastic neurons were observed only in teratomas of patients with paraneoplastic anti-NMDARE, co-expressing NR1, NR2A, NR2B subunits and IgG. Also, CD20 positive B-cells were more common in anti-NMDARE teratomas. In CSF of paraneoplastic anti-NMDARE patients, TNF-α, IL-10 and GM-CSF concentrations were higher than in negative symptom control and VEGF-A and IL-1a were lower than in anti-NMDARE patients (0.25 < p < 0.05).

Conclusions

Patients with teratomas and paraneoplastic anti-NMDARE revealed a cellular population of dysplastic neurons co-expressing NMDAR subunits, which were the potential source of autoantigens triggering anti-NMDARE. Some inflammatory cytokines may be involved in pathogenesis of paraneoplastic anti-NMDARE.
The many faces of Reigate Stone: an assessment of variability in historic masonry based on Medieval London’s principal freestone
Authors: Michette Martin , Viles Heather , Vlachou Constantina , Angus Ian
Published: August 2020

Reigate Stone was used in high profile projects across London during a key growth period and represents an important chapter of architectural heritage. Historic Reigate masonry is subject to inherent variability. It is prone to rapid decay; however, highly decayed and well-preserved stones are frequently adjacent. This inherent variability in masonry can present a challenge to the design of conservation strategies by obscuring or complicating the identification of decay processes. This paper presents a model for assessing the combined impact of construction economies and mineralogical variability (Graphical abstract), by synthesising archival research on the history of Reigate Stone with experimental analysis of its properties. The limitations of the local geography coupled with the demands of the medieval building industry are shown to have introduced inherent variability into the built fabric at an early stage. Later socio-economic factors are shown to have compounded these by contributing to selective recycling, replacement and contamination of Reigate Stone. These historic factors augmented pre-existing mineralogical variability. This variability makes classification according to commonly used stone types difficult. Experimental analysis correlates variable cementing components with hygro-physical properties related to resilience. Calcite content influences strength properties and capillarity; clay content influences moisture adsorption and retention; opal-CT forms a weakly cemented, porous matrix. These presented different decay pathways to a range of environmental mechanisms and agents of decay. The findings suggest that inherent mineralogical variability, environmental changes, and historic contingency must all be considered in the design of ongoing Reigate Stone conservation strategies.
Changing times, changing needs: enhancing the utility of international large-scale assessments
Authors: Kirsch Irwin , Braun Henry
Published: August 2020

Mounting concerns about the levels and distributions of human capital, as well as how they are associated with outcomes for individuals and societies, have contributed to an increase in the number of national and international surveys. These surveys not only examine skills among school-age and adult populations, they also facilitate evaluation of the relationships among these skills and various background factors. At this juncture, the main ILSAs are making the transition to becoming fully digitally based assessments (DBAs). With the transition rapidly progressing, this is a propitious moment to consider the history of large-scale national and international assessments and to reflect on both what has contributed to their increased salience and growth, and how best to enhance their constructive impact on both policy and policy research in the future. We argue this can be done by utilizing a comprehensive, multidimensional framework that establishes a set of design criteria against which these assessments, present and future can be evaluated. The original framework was proposed more than 30 years ago by Messick (European Journal of Psychology of Education II 2:157–165, 1987) when all large-scale assessments were developed and delivered with paper-based instruments. Messick’s framework provided an organizational structure for how to think about and evaluate the potential utility of all large-scale assessments. After presenting a brief historical perspective on the development and growth of large-scale assessments, we review and extend Messick’s original framework. We then describe how the transition to DBAs can impact each of the framework’s three criteria. We also offer recommendations on how ILSAs’ development and innovations can best be deployed so that they are of greater utility to policy makers and other stakeholders worldwide.