bluuebunny/crossref_metadata_2025 huggingface dataset:
Systematic Review and Meta-Analysis of Incidence and Prevalence of Endometriosis
Authors: Sarria-Santamera Antonio , Orazumbekova Binur , Terzic Milan , Issanov Alpamys , Chaowen Chen , Asúnsolo-del-Barco Angel
Published: December 2020

There is still much controversy regarding the epidemiology of endometriosis. The objective of this work is to conduct a systematic review, and if possible, proceed with a meta-analysis of studies that have analyzed the incidence and prevalence of this condition among women in the general population. The inclusion criteria were papers published after 1997 that had reported data of the incidence or prevalence of endometriosis. The PubMed search engine was used to identify papers meeting the inclusion criteria from 1997 to 2019, with an additional manual search for the identification of potentially eligible studies. The search was limited to papers published in English. The risk of bias was assessed according to the Joanna Briggs Institute Critical Appraisal Checklist. As a result, 27 papers, which included a total of 28,660,652 women, were classified according to the type of design and sources of information in five subgroups. Pooled estimates of prevalence for studies with self-reported data were 0.05 (95% CI: 0.03; 0.06), 0.01 for population-based integrated information systems (95% CI: 0.01; 0.02), and 0.04 (95% CI 0.04; 0.05) in studies using other designs. The pooled incidence rate of endometriosis was: 1.36 per 1000 person-years (PY) (95% CI: 1.09; 1.63) for studies based on hospital discharges, 3.53 per 1000 PY (95% CI: 2.06; 4.99) for cohort studies, and 1.89 per 1000 PY (95% CI: 1.42; 2.37) for population-based integrated information systems. Meta-analysis indicated high heterogeneity based on I-squared statistics. This significant variability may not only be due to methodological issues and the specific limitations of the different designs and data analyzed, including case definitions and subject selection strategies, but also to the inherent heterogeneity of endometriosis. Epidemiological studies with appropriate study designs remain necessary to provide a valid estimation of the population burden of endometriosis.
Freezing Tolerance and Chilling Fulfillment Differences in Cold Climate Grape Cultivars
Authors: Yilmaz Turhan , Alahakoon Dilmini , Fennell Anne
Published: December 2020

Grapevine sustainability is impacted by the timing of dormancy initiation and freezing tolerance in fall and winter and chilling fulfillment and bud break in the spring. These traits have genetic and local temperature contributing factors; therefore, this study was undertaken to develop an understanding of these characteristics in four recently developed cold climate cultivars. The cold hardiness and chilling fulfillment profiles were monitored in Brianna, Frontenac gris, La Crescent and Marquette using differential thermal analyses and bud break assays. Bud cold hardiness of all cultivars increased with the declining temperatures from November through February, after which the buds began to lose freezing tolerance. There were significant differences in cold hardiness and chilling fulfillment between cultivars during the endodormant and ecodormant period of winter. Marquette had the greatest freezing tolerance from early November through midwinter suggesting it has potential as a sentinel cultivar for comparisons of new cold climate selections. Brianna was slower to acclimate and deacclimated more rapidly than the other cultivars. Chilling fulfillment under natural field or constant 4 °C conditions showed no main effect differences for chilling accumulation condition; however, there were significant cultivar, condition, and time point interactions, indicating the cultivars differed in chilling fulfillment responses.
Functional MRI (fMRI) Evaluation of Hyperbaric Oxygen Therapy (HBOT) Efficacy in Chronic Cerebral Stroke: A Small Retrospective Consecutive Case Series
Authors: Cevolani Daniela , Di Donato Ferruccio , Santarella Luigi , Bertossi Simone , Cellerini Martino
Published: December 2020

Topics: Functional Magnetic Resonance Imaging (fMRI) evaluation of HyberBaric Oxygen Therapy (HBOT) effects on chronic cerebral stroke Patients (Pts). Introduction: Our aim was to evaluate with fMRI, in a 3 Tesla system, the functional effects of HBOT on the Central Nervous System (CNS) in four Pts with established ischaemic and haemorrhagic cerebral strokes (2 Pts each). To our knowledge, no author used fMRI technique for this purpose, till now. Methods: All four Pts underwent a fMRI study before and after 40 HBOT sessions, with a time window of a few days. They carried out two language (text listening, silent word-verb generation) and two motor (hand and foot movements) tasks (30 s On-Off block paradigms). Results: After HBOT, all Pts reported a clinical improvement, mostly concerning language fluency and motor paresis. fMRI analysis demonstrated an increase in both the extent and the statistical significance of most of the examined eloquent areas. Conclusions: These changes were consistent with the clinical improvement in all Pts, suggesting a possible role of fMRI in revealing neuronal functional correlates of neuronal plasticity and HBOT-related neoangiogenesis. Although only four Pts were examined, fMRI proved to be a sensitive, non-invasive and reliable modality for monitoring neuronal functional changes before and after HBOT.
Chinese Students’ Health Literacy Level and Its Associated Factors: A Meta-Analysis
Authors: Mao Ying , Xie Tao , Zhang Ning
Published: December 2020

Health Literacy (HL) is an important determinant of health. Many scholars have conducted a large number of studies on the level of Chinese students’ HL and its associated factors. However, previous studies on HL level and the factors that influence it have been contradictory. Therefore, this systematic review and meta-analysis was conducted to estimate the level of Chinese students’ HL and its three dimensions (knowledge, behavior and skills) and to identify factors associated with HL in Chinese students. Two investigators independently searched literature, selected research and extracted data through comprehensively searching of four international electronic databases and three Chinese electronic database to identify all relevant observational studies on affecting factors for HL in Chinese students published in English and Chinese from database January, 2010 to September, 2020. In total, 61 articles were extracted in the study. The results showed that the level rates of HL and its three dimensions were 26%, 35%, 26%, 51%, respectively. For Chinese students, the significant factors were urban residents, senior class students, well performance at school, the Han nationality, focus on health knowledge, less exposure to video games, highly educated parents, income of one-child families, receiving health education, having medical background. This study provides some inspirations for improving the level of Chinese students’ HL and their health. First, the findings may help Chinese policy makers understand the overall HL of Chinese students and their levels across three dimensions (knowledge, behavior and skills). Second, protective factors for Chinese students’ HL were found in this research, which will help to improve the level of Chinese students’ HL, stimulate students’ awareness of prevention, and lay the foundation for a healthy China.
Histological and Radiological Features of a Four-Phase Injectable Synthetic Bone Graft in Guided Bone Regeneration: A Case Report
Authors: Čandrlić Marija , Perić Kačarević Željka , Ivanišević Zrinka , Tomas Matej , Včev Aleksandar , Faj Dario , Matijević Marko
Published: December 2020

Background and objective: Injectable synthetic bone grafts (ISBG) are widely used biomaterials for regeneration purposes. The aim of this case report was to examine the efficacy of ISBG in the management of buccal fenestration in the case of a 25-year-old female. Case report: After a traumatic tooth extraction, the defect was filled with ISBG and covered with a resorbable membrane. The ISBG showed easy handling and the patient had no complications during healing. Six months after augmentation, a bone biopsy was taken during implant bed preparation. The histological results showed good integration of ISBG into the newly formed bone and no signs of tissue inflammation. Additionally, a CBCT (cone beam computed tomography) analysis was performed to support the histological results. Conclusion: The use of the examined ISBG led to successful treatment of the buccal fenestration defect.
Response to Flavored Cartridge/Pod-Based Product Ban among Adult JUUL Users: “You Get Nicotine However You Can Get It”
Authors: Yingst Jessica M. , Bordner Candace R. , Hobkirk Andréa L. , Hoglen Brianna , Allen Sophia I. , Krebs Nicolle M. , Houser Kenneth R. , Livelsberger Craig , Foulds Jonathan
Published: December 2020

In order to curb increasing youth electronic cigarette (e-cig) use, the United States Food and Drug Administration (FDA) banned the sale of flavored cartridge/pod-based products in February 2020. This mixed-methods study aimed to evaluate the impact of the FDA ban on adult JUUL users. The samples of current adult JUUL users were surveyed via Amazon Mechanical Turk at three time-points n = 76 (Sample 1); n = 128 (Sample 2); n = 86 (Sample 3) before and after the FDA flavored/pod ban. The participants were asked to report the JUUL flavored pod used most often and answer questions on purchasing generic pods or refilling (Quantitative). JUUL users were then interviewed in order to explore their perceptions and behaviors related to the FDA ban of flavored cartridge/pod-based products (Qualitative; n = 16). Quantitative data analysis evaluated the differences in variables by time-point. Qualitative data were coded into themes while using the constant comparative method. We found a significant decrease in the use of mint pods (43.4%, 22.7%, 16.3%) (p < 0.01), while there was a significant increase in the use of menthol pods (6.6%, 26.6%, 37.2%) (p < 0.01). Themes that emerged from the qualitative data included switching from mint to menthol pods, refilling pods, and switching to other products that are available in the desired flavors, such as generic pods or disposable e-cigs. Future research is needed in order to evaluate the impact of these behaviors on public health.
The Influence of Urban Park Attributes on User Preferences: Evaluation of Virtual Parks in an Online Stated-Choice Experiment
Authors: van Vliet Esther , Dane Gamze , Weijs-Perrée Minou , van Leeuwen Eveline , van Dinter Mayke , van den Berg Pauline , Borgers Aloys , Chamilothori Kynthia
Published: December 2020

Urban green areas, such as parks, are becoming increasingly important in densifying cities. Urban parks encourage physical and social activity, recreation and relaxation, and thus eventually promote people’s well-being. The aim of the current study is to examine which urban park attributes influence the preferences of park users, in order to offer recommendations regarding how urban parks of quality can be designed. To elicit the preferences of park visitors we designed an online stated-choice experiment. Seven park attributes, in particular the number and composition of trees and the presence of benches, side paths, a playground, litter, and flowers, were manipulated in a virtual park. In an online stated-choice task, videos of these park alternatives were presented and the preferences of 697 participants were measured. It is found that especially the number of trees and the presence of flowerbeds, particularly with a diversity of flowers, influenced participants’ preferences. The presence of many benches and a playground were valued as well, but to a lesser extent. The presence of litter was found to be less troublesome than expected. Alternatives with all trees placed in one cluster were disliked. Moreover, significant standard deviations were found for the presence of side paths, a playground, and the absence of litter, which indicates that preference heterogeneity for these attributes exist. In a latent class analysis, two groups were identified, namely a Nature-loving group, who mainly valued the trees and the flowers, and an Amenity-appreciating group, who valued almost all attributes. It can be concluded that natural elements and a variety of flower species are important in an urban park, while facilities are evaluated differently by different groups of people. These findings may support park designers and policymakers in decision-making. Moreover, it illustrates the usefulness of creating a virtual park in environmental preference research.
Training-Induced Acute Neuromuscular Responses to Military Specific Test during a Six-Month Military Operation
Authors: Pihlainen Kai , Pesola Arto J , Helén Joonas , Häkkinen Keijo , Finni Taija , Ojanen Tommi , Vaara Jani P. , Santtila Matti , Raitanen Jani , Kyröläinen Heikki
Published: December 2020

Limited data are available regarding strength and endurance training adaptations to occupational physical performance during deployment. This study assessed acute training-induced changes in neuromuscular (electromyography; EMG) and metabolic (blood lactate, BLa) responses during a high-intensity military simulation test (MST), performed in the beginning (PRE) and at the end (POST) of a six-month crisis-management operation. MST time shortened (145 ± 21 vs. 129 ± 16 s, −10 ± 7%, p < 0.001) during the operation. Normalized muscle activity increased from PRE to POST in the hamstring muscles by 87 ± 146% (116 ± 52 vs. 195 ± 139%EMGMVC, p < 0.001) and in the quadriceps by 54 ± 81% (26 ± 8 vs. 40 ± 20%EMGMVC, p < 0.001). In addition, higher acute BLa values were measured after MST during POST. Changes in BLa and EMG suggested an increased neural input and metabolic rate during POST MST, likely leading to faster performance times at the end of the operation. High EMG values throughout the different phases of MST suggested that despite the anaerobic nature of the test, the soldiers were able to maintain their voluntary muscle activation level until the end of the test. This indicates only limited neural fatigue during the two-minute high-intensity military specific performance. While learning effect may explain some part of the improvement in the MST performance times, combined strength and endurance training three times per week may improve neuromuscular performance in occupationally relevant tasks.
A Sentiment Analysis Approach to Predict an Individual’s Awareness of the Precautionary Procedures to Prevent COVID-19 Outbreaks in Saudi Arabia
Authors: Aljameel Sumayh S. , Alabbad Dina A. , Alzahrani Norah A. , Alqarni Shouq M. , Alamoudi Fatimah A. , Babili Lana M. , Aljaafary Somiah K. , Alshamrani Fatima M.
Published: December 2020

In March 2020, the World Health Organization (WHO) declared the outbreak of Coronavirus disease 2019 (COVID-19) as a pandemic, which affected all countries worldwide. During the outbreak, public sentiment analyses contributed valuable information toward making appropriate public health responses. This study aims to develop a model that predicts an individual’s awareness of the precautionary procedures in five main regions in Saudi Arabia. In this study, a dataset of Arabic COVID-19 related tweets was collected, which fell in the period of the curfew. The dataset was processed, based on several machine learning predictive models: Support Vector Machine (SVM), K-nearest neighbors (KNN), and Naïve Bayes (NB), along with the N-gram feature extraction technique. The results show that applying the SVM classifier along with bigram in Term Frequency–Inverse Document Frequency (TF-IDF) outperformed other models with an accuracy of 85%. The results of awareness prediction showed that the south region observed the highest level of awareness towards COVID-19 containment measures, whereas the middle region was the least. The proposed model can support the medical sectors and decision-makers to decide the appropriate procedures for each region based on their attitudes towards the pandemic.
The Effects of Physicochemical Parameters on Analysed Soil Enzyme Activity from Alice Landfill Site
Authors: Maphuhla Nontobeko Gloria , Lewu Francis Bayo , Oyedeji Opeoluwa Oyehan
Published: December 2020

The soil pollution as a product of xenobiotics, industrial action, agricultural chemicals, or inappropriate disposal of waste can change the natural environment of soil indices and trigger life-threatening situations. Soil enzyme activity is the suitable bio-indicator or parameter for monitoring soil pollution due to their sensitivity that quickly responds to any soil disturbances. Also, they are known to play an essential role in maintaining soil health and a quality environment. This study aimed to determine the levels of enzyme activity in soil from polluted and unpolluted sites and study the relationship between the physicochemical properties and soil enzyme activity to manage soil pollution. Four selected enzymes (Urease, Invertase, Catalase, and Phosphatase) were examined for their activity from forty samples using the assay method for 24 h; the colorimetry spectrophotometer measured their activity. The obtained data revealed that Invertase activity was a predominant enzyme in all soil samples. Whereas, the urease activity had obtained in low amounts for all collection sites, especially on Site A1. Soil pH had discovered to range between 5.8 and 8.51, moisture content values recorded to vary from 0.12% to 9.09%, and soil organic carbon recorded to fluctuate between 0.08% and 1.54%. Urease and phosphatase activity correlated positively with all soil physicochemical properties except for moisture content, which correlated negatively (r = −0.297; p ≥ 0.05). The invertase activity negatively associated all soil physicochemical properties, excluding the moisture content that correlated positively and significantly with invertase activity. We noted that the dumpsite soil contains low enzyme activity levels, which might attribute to the type of waste disposed off. Also, only the phosphatase activity reported correlating positively with all examined physicochemical parameters entirely.
Epidemiological Challenges in Rare Bleeding Disorders: FVIII Inhibitor Incidence in Haemophilia A Patients—A Known Issue of Unknown Origin
Authors: Keipert Christine , Drechsel-Bäuerle Ursula , Oberle Doris , Müller-Olling Mirco , Hilger Anneliese
Published: December 2020

There is a broad range of factor products approved in Germany for haemophilia A treatment. Since the introduction of recombinant coagulation factor VIII (FVIII) products in the 1990s, there has been substantial debate whether there is a difference in inhibitor incidence between single FVIII products or product classes. Neither haemophilia registries nor clinical studies, including a randomised controlled clinical trial, provided a consistent and definite answer. The reasons were mainly related to methodological challenges in conducting controlled studies in a rare disease. In this analysis, the most relevant epidemiological challenges and main problems were examined, including study bias, potential overlap of individual studies and advanced development of therapy and methods in the course of time. Meta-analyses on two levels showed that therapies using recombinant products resulted in different event rates when compared to plasma-derived products. These results are accompanied by substantial study heterogeneity evidenced by Cochran’s Q tests. Only three studies have been identified that meet the standards of current clinical guidance. To finally resolve this ongoing and disputable safety issue of replacement therapy, collaboration among registry owners, academia and regulators must be fostered.
Deeper Spatial Statistical Insights into Small Geographic Area Data Uncertainty
Authors: Griffith Daniel A. , Chun Yongwan , Lee Monghyeon
Published: December 2020

Small areas refer to small geographic areas, a more literal meaning of the phrase, as well as small domains (e.g., small sub-populations), a more figurative meaning of the phrase. With post-stratification, even with big data, either case can encounter the problem of small local sample sizes, which tend to inflate local uncertainty and undermine otherwise sound statistical analyses. This condition is the opposite of that afflicting statistical significance in the context of big data. These two definitions can also occur jointly, such as during the standardization of data: small geographic units may contain small populations, which in turn have small counts in various age cohorts. Accordingly, big spatial data can become not-so-big spatial data after post-stratification by geography and, for example, by age cohorts. This situation can be ameliorated to some degree by the large volume of and high velocity of big spatial data. However, the variety of any big spatial data may well exacerbate this situation, compromising veracity in terms of bias, noise, and abnormalities in these data. The purpose of this paper is to establish deeper insights into big spatial data with regard to their uncertainty through one of the hallmarks of georeferenced data, namely spatial autocorrelation, coupled with small geographic areas. Impacts of interest concern the nature, degree, and mixture of spatial autocorrelation. The cancer data employed (from Florida for 2001–2010) represent a data category that is beginning to enter the realm of big spatial data; its volume, velocity, and variety are increasing through the widespread use of digital medical records.
Development and Effects of College-Based Lifestyle Modification Program for Menstrual Health of Young Adult Women with Irregular Menses: A Randomized Controlled Trial
Authors: Park Young-Joo , Shin Hyunjeong , Jeon Songi , Cho Inhae , Park Hyun Ji
Published: December 2020

Purpose: This study was conducted to develop the ‘College-based Lifestyle Modification Program’ (College-based LMP) for young adult women with irregular menstruation and examine its effects after intervention. Methods: The College-based LMP consisted of small group education, individual physical exercise counseling/training, individual diet counseling, and feedback and support. Participants were comprised of 38 females who reported less than 10 irregular menstruations in a year and were randomly assigned to the experimental and control groups. The primary outcome variables consisted of menstrual cycle index (MCI), sex hormone binding globulin (SHBG), and androgenic profile (testosterone—T, free androgen index—FAI), while the outcome variables included premenstrual symptoms (PMS), menstrual volume, body composition parameters, glycemic parameters (fasting blood sugar—FBS, insulin, HOMA-IR), sleep duration, perceived stress, and nutrient intake.. Results: There were no significant differences in primary outcome variables (MCI, SHBG, T, and FAI). In the variables, there were no significant differences except for the partial domain of PMS (symptoms of depression and anxiety) and sleep duration. Conclusions: The study was significant in that it demonstrated the importance of lifestyle, which could provide ordinary young adult women with healthy menstruation. The College-based LMP needs to be elaborated with further studies.
Where Do Cancer Patients in Receipt of Home-Based Palliative Care Prefer to Die and What Are the Determinants of a Preference for a Home Death?
Authors: Cai Jiaoli , Zhang Li , Guerriere Denise , Fan Hongli , Coyte Peter C.
Published: December 2020

Understanding the preferred place of death may assist to organize and deliver palliative health care services. The study aims to assess preference for place of death among cancer patients in receipt of home-based palliative care, and to determine the variables that affect their preference for a home death. A prospective cohort design was carried out from July 2010 to August 2012. Over the course of their palliative care trajectory, a total of 303 family caregivers of cancer patients were interviewed. Multivariate regression analysis was employed to assess the determinants of a preferred home death. The majority (65%) of patients had a preference of home death. The intensity of home-based physician visits and home-based personal support worker (PSW) care promotes a preference for a home death. Married patients, patients receiving post-graduate education and patients with higher Palliative Performance Scale (PPS) scores were more likely to have a preference of home death. Patients reduced the likelihood of preferring a home death when their family caregiver had high burden. This study suggests that the majority of cancer patients have a preference of home death. Health mangers and policy makers have the potential to develop policies that facilitate those preferences.
The Role of Haptoglobin Polymorphism in Cardiovascular Disease in the Setting of Diabetes
Authors: Somer Shmuel , Levy Andrew P.
Published: December 2020

Atherosclerotic cardiovascular disease (CVD) is the major cause of morbidity and mortality in individuals with diabetes mellitus (DM). Preclinical models have suggested that excessive oxidative stress and hyperglycemia are directly responsible for this pathological association. However, numerous clinical trials involving the administration of high doses of the antioxidant vitamin E or attempts at strict glycemic control have failed to show a significant reduction of CVD in DM patients. We describe here a possible explanation for the failure of these trials, that being their lack of proper patient selection. The haptoglobin (Hp) genotype is a major determinant of the risk of CVD in the setting of DM. Treatment of individuals with the high-risk Hp genotype with antioxidants or aggressive glycemic control has shown benefit in several small studies. These studies suggest a precision medicine-based approach to preventing diabetes complications. This approach would have a profound effect on the costs of diabetes care and could dramatically reduce morbidity from diabetes.
Surgical Treatment of Intrahepatic Cholangiocarcinoma: Current and Emerging Principles
Authors: Beal Eliza W. , Cloyd Jordan M. , Pawlik Timothy M.
Published: December 2020

Intrahepatic cholangiocarcinoma (ICC) is a rare, aggressive cancer of the biliary tract. It often presents with locally advanced or metastatic disease, but for patients with early-stage disease, surgical resection with negative margins and portahepatis lymphadenectomy is the standard of care. Recent advancements in ICC include refinement of staging, improvement in liver-directed therapies, clarification of the role of adjuvant therapy based on new randomized controlled trials, and advances in minimally invasive liver surgery. In addition, improvements in neoadjuvant strategies and surgical techniques have enabled expanded surgical indications and reduced surgical morbidity and mortality. However, recurrence rates remain high and more effective systemic therapies are still necessary to improve recurrence-free and overall survival. In this review, we focus on current and emerging surgical principals for the management of ICC including preoperative evaluation, current indications for surgery, strategies for future liver remnant augmentation, technical principles, and the role of neoadjuvant and adjuvant therapies.
Biocompatibility and Biological Corrosion Resistance of Ti–39Nb–6Zr+0.45Al Implant Alloy
Authors: Hwang Yu-Jin , Choi Young-Sin , Hwang Yun-Ho , Cho Hyun-Wook , Lee Dong-Geun
Published: December 2020

Titanium and titanium alloys are promising implant metallic materials because of their high strengths, low elastic moduli, high corrosion resistances, and excellent biocompatibilities. A large difference in elastic modulus between the implant material and bone leads to a stress shielding effect, which increases the probability of implant separation or decrease in the bone density around it. Thus, a lower elastic modulus is required for a better implant metallic material. β titanium has a lower elastic modulus and high strength and can reduce the probability of the stress shielding effect. In this study, the applicability of the Ti–39Nb–6Zr+0.45Al alloy, obtained by adding a small amount of aluminum to the Ti–39Nb–6Zr alloy, as a biomedical implant material was evaluated. The mechanical properties and biocompatibility of the alloy were evaluated. The biocompatibility of Ti–39Nb–6Zr+0.45Al was similar to that of Ti–39Nb–6Zr according to in vitro and in vivo experiments. In addition, the biological corrosion resistances were evaluated through a corrosion test using a 0.9% NaCl solution, which is equivalent to physiological saline. The corrosion resistance was improved by the addition of Al. The yield strength of the Ti–39Nb–6Zr+0.45Al alloy was improved by approximately 20%. The excellent biocompatibility confirmed its feasibility for use as a biomedical implant material.
Development of Depth-Limited Wave Boundary Layers over a Smooth Bottom
Authors: Tanaka Hitoshi , Tinh Nguyen Xuan , Yu Xiping , Liu Guangwei
Published: December 2020

A theoretical and numerical study is carried out to investigate the transformation of the wave boundary layer from non-depth-limited (wave-like boundary layer) to depth-limited one (current-like boundary layer) over a smooth bottom. A long period of wave motion is not sufficient to induce depth-limited properties, although it has simply been assumed in various situations under long waves, such as tsunami and tidal currents. Four criteria are obtained theoretically for recognizing the inception of the depth-limited condition under waves. To validate the theoretical criteria, numerical simulation results using a turbulence model as well as laboratory experiment data are employed. In addition, typical field situations induced by tidal motion and tsunami are discussed to show the usefulness of the proposed criteria.
Quantifying the Landscape’s Ecological Benefits—An Analysis of the Effect of Land Cover Change on Ecosystem Services
Authors: Ureta J. Carl , Clay Lucas , Motallebi Marzieh , Ureta Joan
Published: December 2020

The increasing pressure from land cover change exacerbates the negative effect on ecosystems and ecosystem services (ES). One approach to inform holistic and sustainable management is to quantify the ES provided by the landscape. Using the Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) model, this study quantified the sediment retention capacity and water yield potential of different land cover in the Santee River Basin Network in South Carolina, USA. Results showed that vegetated areas provided the highest sediment retention capacity and lowest water yield potential. Also, the simulations demonstrated that keeping the offseason crop areas vegetated by planting cover crops improves the monthly ES provision of the landscape. Retaining the soil within the land area prevents possible contamination and siltation of rivers and streams. On the other hand, low water yield potential translates to low occurrence of surface runoff, which indicates better soil erosion control, regulated soil nutrient absorption and gradual infiltration. The results of this study can be used for landscape sustainability management to assess the possible tradeoffs between ecological conservation and economic development. Furthermore, the generated map of ES can be used to pinpoint the areas where ES are best provided within the landscape.
Directly Acting Antiviral-Based Treatment for HCV-Infected Persons Who Inject Drugs: A Multicenter Real-Life Study
Authors: Messina Vincenzo , Onorato Lorenzo , Di Caprio Giovanni , Claar Ernesto , Iovinella Vincenzo , Russo Antonio , Rosato Valerio , Salzillo Angela , Nevola Riccardo , Simeone Filomena , Curcio Fabio , Pisaturo Mariantonietta , Coppola Nicola
Published: December 2020

Background: We aimed to evaluate the factors associated with a virological response in a cohort of Hepatitis C virus (HCV)-infected people who inject drugs (PWID) treated with direct acting antivirals (DAAs). Methods: We conducted a multicenter retrospective cohort study enrolling HCV-infected PWID treated with DAAs. The primary outcome evaluated was the sustained virological response (SVR12) rate. Results: Five hundred and twenty HCV-infected PWID treated with all-oral DAA-based regimens were enrolled; a total of 168 (32.3%) patients presented genotype 1a, 109 (21.0%) genotype 1b, and 174 (33.5%) genotype 3; a total 152 of the 520 subjects (29.2%) were cirrhotics; a total 118 (22.7%) and 373 (71.7%) were treated with DAA regimens of second and third generation, respectively; a total 169 (33.6%) patients were receiving an opioid agonist at the start of antiviral therapy. Only 11 subjects (2.1%) did not show an SVR12. A significant correlation was found between treatment with opioid substitution therapy (p < 0.001), Human Immunodeficiency Virus (HIV) coinfection (p = 0.002), and treatment with first- or second-generation regimens (p = 0.0015) and HCV failure. Upon multivariate analysis, treatment with a first- or second-generation DAA was the only factor independently associated with failure (OR 10.4, 95% CI: 1.43 to 76.1, p = 0.02). Conclusions: Treatment with DAAs led to a high SVR12 rate (97.9%) in a large cohort of HCV-infected PWID. The only predictor of viral failure found in our analysis was treatment with first- and second-generation DAA.
Numerical Modelling of CFS Three-Story Strap-Braced Building under Shaking-Table Excitations
Authors: Campiche Alessia
Published: December 2020

In recent research activities, shake-table tests were revealed to be useful to investigate the seismic behavior of cold-formed steel (CFS) buildings. However, testing full-scale buildings or reduced-scale prototypes is not always possible; indeed, predicting tools and numerical models could help designers to evaluate earthquake response. For this reason, numerical modelling of two strap-braced prototype buildings, recently tested on shake-table at University of Naples Federico II in cooperation with Lamieredil S.p.A. company, was developed. The models were validated trough the comparison between experimental and numerical results, in term of dynamic properties (fundamental period of vibration and modal shapes), peak roof drift ratios and peak inter-story drift ratios. Although dynamic properties of mock-ups were captured with accuracy by the developed models, the comparison highlighted the need to consider accumulation of damage and rocking phenomenon in the modelling to capture with good accuracy the seismic behavior of CFS strap-braced building, subjected to high intensity records.
Fuzzy Stochastic Automation Model for Decision Support in the Process Inter-Budgetary Regulation
Authors: Yakovenko Irina
Published: December 2020

The purpose of this article is to study the theoretical foundations of the concept of fiscal decentralization, as the main path of self-development of the national economy of any country, and to develop mathematical tools that support decision-making in the aspect of “hard” budget constraints. The study of the problems of fiscal policy formation in foreign countries presented in modern scientific literature has revealed that the degree of application of the concepts of “soft” and “hard” budget restrictions is an actual topic in the theory of fiscal federalism. It has been substantiated that decision-making within the framework of “soft” budget constraints (financial assistance) leads to low tax autonomy of territories and limited liability of regional and municipal authorities for the results of their financial policy. As a research hypothesis, we put forward the thesis that it is necessary to create conditions for encouraging subnational authorities to support the territorial economy by granting them the possibility to use part of the taxes collected in the respective territories. The implementation of this thesis has given rise to the problem of quantifying decisions made regarding the establishment of standards for the distribution of tax revenues between budgets of different levels of the hierarchy of the country’s budget system. In terms of solving this problem, the author has constructed mathematical models based on the use of synthesis of mathematical apparatus of the theory of stochastic automata, fuzzy algebra, and simulation. In terms of solving this problem, the author proposed the use of mathematical modeling methods. The article presents the results of constructing economic and mathematical models to support decision-making in the vertical distribution of tax revenues between budgets. The models include stochastic automata, as mathematical abstractions, describing the expedient behavior of an economic agent when choosing management alternatives for territories of different levels of economic development. The transition functions of automaton models are formally described on the basis of the synthesis of mathematical apparatus of the theories of stochastic automata operating in random environments and fuzzy sets. The expediency property of the behavior of automaton models is justified by proving the corresponding theorems. The random environment in which stochastic automata are immersed is formed by a simulation model. The article demonstrates the results of experiments carried out on models, as well as a conceptual scheme of interaction between the automaton and simulation models.
Nonspecific Low Back Pain among Kyokushin Karate Practitioners
Authors: Błach Wiesław , Klimek Bartosz , Rydzik Łukasz , Ruzbarsky Pavel , Czarny Wojciech , Raś Ireneusz , Ambroży Tadeusz
Published: December 2020

Background and objective: Spinal pain is a common and growing problem, not only in the general population but also among athletes. Lifestyle, occupation, and incorrectly exerted effort have a significant impact on low back pain. To assess the prevalence of low back pain among those practicing Kyokushin karate, we take into account age, body weight, sex, length of karate experience, level of skill, and occupation. Materials and Methods: The study involved 100 people practicing Kyokushin karate, aged 18 to 44. A questionnaire developed for this study and the Oswestry Disability Index (ODI) were used. Results: The research showed the prevalence of low back pain among karate practitioners (55%), depending on age (R = −0.24; p = 0.015), body weight (χ2 = 16.7; p = 0.002), occupation (χ2 = 18.4; p = 0.0004), and overall length of karate experience (R = −0.28; p = 0.04). A correlation was also found between sex (χ2 = 22.3; p = 0.001), occupation (χ2 = 51; p = 0.0000), length of experience (R = −0,28; p = 0.04), karate skill level (R = 0.39; p = 0.003), and the intensity of pain defined using the Visual Analogue Scale (VAS). Subjects with low back pain showed minimal (71%) and moderate (29%) disability according to the Oswestry index. Conclusions: Low back pain is common in karate practitioners and depends on age, weight, occupation, and length of karate experience. The intensity of low back pain is influenced by sex, occupation, overall length of training experience, and one’s level of karate skill. Lumbar spine ailments reduce functionality and quality of life to a small degree. Karate practitioners seldom seek treatment for spinal pains, and only few use physiotherapy and pharmacology.
Development of Tandem Mass Tag Labeling Method for Lipid Molecules Containing Carboxy and Phosphate Groups, and Their Stability in Human Serum
Authors: Tokuoka Suzumi M. , Kita Yoshihiro , Sato Masaya , Shimizu Takao , Yatomi Yutaka , Oda Yoshiya
Published: December 2020

In clinical lipidomics, it is a challenge to measure a large number of samples and to reproduce the quantitative results. We expanded the range of application of the tandem mass tag (TMT) method, which is widely used in proteomics, to lipidomic fields. There are various types of lipid molecule, for example, eicosanoids have a carboxyl group and phosphatidic acid has a phosphate group. We modified these functional groups simultaneously with TMT. This approach allows for a single analysis by mixing six samples and using one of the six samples as a bridging sample; the quantitative data can be easily normalized even if the number of measurements increases. To accommodate a large number of samples, we utilize a pooled serum sample of 300 individuals as a bridging sample. The stability of these lipid molecules in serum was examined as an analytical validation for the simultaneous TMT labeling. It was found that the stability of these lipid molecules in serum differs greatly depending on the lipid species. These findings reaffirmed the importance of proper sample preparation and storage to obtain reliable data. The TMT labeling method is expected to be a useful method for lipidomics with high-throughput and reliable reproducibility.
Probiotic Alternative to Chlorhexidine in Periodontal Therapy: Evaluation of Clinical and Microbiological Parameters
Authors: Butera Andrea , Gallo Simone , Maiorani Carolina , Molino Domenico , Chiesa Alessandro , Preda Camilla , Esposito Francesca , Scribante Andrea
Published: December 2020

Periodontitis consists of a progressive destruction of tooth-supporting tissues. Considering that probiotics are being proposed as a support to the gold standard treatment Scaling-and-Root-Planing (SRP), this study aims to assess two new formulations (toothpaste and chewing-gum). 60 patients were randomly assigned to three domiciliary hygiene treatments: Group 1 (SRP + chlorhexidine-based toothpaste) (control), Group 2 (SRP + probiotics-based toothpaste) and Group 3 (SRP + probiotics-based toothpaste + probiotics-based chewing-gum). At baseline (T0) and after 3 and 6 months (T1–T2), periodontal clinical parameters were recorded, along with microbiological ones by means of a commercial kit. As to the former, no significant differences were shown at T1 or T2, neither in controls for any index, nor in the experimental groups for adherent gingiva and gingival recession. Conversely, some significant differences were found in Group 2 and 3 for the other clinical indexes tested. Considering microbiological parameters, no significant differences were detected compared to baseline values for any group, except in Group 2 and 3 at T2 only for the percentage of the orange complex pathogens and for the copies/microliter of Prevotella intermedia and Fusobacterium nucleatum. Accordingly, although colonization of probiotic bacteria has not been assessed in this study, the probiotics tested represent a valid support to SRP with a benefit on several clinical indexes and on specific periodontopathogens. Despite this promising action, the relationship between the use of probiotics and improvement in clinical parameters is still unclear and deserves to be further explored.
Detection of Epstein–Barr Virus in Periodontitis: A Review of Methodological Approaches
Authors: Tonoyan Lilit , Chevalier Marlène , Vincent-Bugnas Séverine , Marsault Robert , Doglio Alain
Published: December 2020

Periodontitis, an inflammatory condition that affects the structures surrounding the tooth eventually leading to tooth loss, is one of the two biggest threats to oral health. Beyond oral health, it is associated with systemic diseases and even with cancer risk. Obviously, periodontitis represents a major global health problem with significant social and economic impact. Recently, a new paradigm was proposed in the etiopathogenesis of periodontitis involving a herpesviral–bacterial combination to promote long-term chronic inflammatory disease. Periodontitis as a risk factor for other systemic diseases can also be better explained based on viral–bacterial etiology. Significant efforts have brought numerous advances in revealing the links between periodontitis and Epstein–Barr virus (EBV), a gamma herpesvirus ubiquitous in the adult human population. The strong evidence from these studies may contribute to the advancement of periodontitis research and the ultimate control of the disease. Advancing the periodontitis research will require implementing suitable methods to establish EBV involvement in periodontitis. This review evaluates and summarizes the existing methods that allow the detection and diagnosis of EBV in periodontitis (also applicable in a more general way to other EBV-related diseases), and discusses the feasibility of the application of innovative emerging technologies.
Probiogenomics Analysis of 97 Lactobacillus crispatus Strains as a Tool for the Identification of Promising Next-Generation Probiotics
Authors: Fontana Federico , Alessandri Giulia , Lugli Gabriele Andrea , Mancabelli Leonardo , Longhi Giulia , Anzalone Rosaria , Viappiani Alice , Ventura Marco , Turroni Francesca , Milani Christian
Published: December 2020

Members of the genus Lactobacillus represent the most common colonizers of the human vagina and are well-known for preserving vaginal health and contrasting the colonization of opportunistic pathogens. Remarkably, high abundance of Lactobacillus crispatus in the vaginal environment has been linked to vaginal health, leading to the widespread use of many L. crispatus strains as probiotics. Nevertheless, despite the scientific and industrial relevance of this species, a comprehensive investigation of the genomics of L. crispatus taxon is still missing. For this reason, we have performed a comparative genomics analysis of 97 L. crispatus strains, encompassing 16 strains sequenced in the framework of this study alongside 81 additional publicly available genome sequences. Thus, allowing the dissection of the L.crispatus pan-genome and core-genome followed by a comprehensive phylogenetic analysis based on the predicted core genes that revealed clustering based on ecological origin. Subsequently, a genomics-targeted approach, i.e., probiogenomics analysis, was applied for in-depth analysis of the eight L. crispatus strains of human origin sequenced in this study. In detail their genetic repertoire was screened for strain-specific genes responsible for phenotypic features that may guide the identification of optimal candidates for next-generation probiotics. The latter includes bacteriocin production, carbohydrates transport and metabolism, as well as a range of features that may be responsible for improved ecological fitness. In silico results regarding the genetic repertoire involved in carbohydrate metabolism were also validated by growth assays on a range of sugars, leading to the selection of putative novel probiotic strains.
Comparative Genomics and In Vitro Plant Growth Promotion and Biocontrol Traits of Lactic Acid Bacteria from the Wheat Rhizosphere
Authors: Strafella Sabrina , Simpson David J. , Yaghoubi Khanghahi Mohammad , De Angelis Maria , Gänzle Michael , Minervini Fabio , Crecchio Carmine
Published: December 2020

This study aimed to isolate lactic acid bacteria (LAB) from wheat rhizosphere, to characterize their in vitro plant growth promoting activities and to differentiate plant-associated LAB from those associated with foods or human disease through comparative genomic analysis. Lactococcus lactis subsp. lactis and Enterococcus faecium were isolated using de Man-Rogosa-Sharpe (MRS) and Glucose Yeast Peptone (GYP) as enrichment culture media. Comparative genomic analyses showed that plant-associated LAB strains were enriched in genes coding for bacteriocin production when compared to strains from other ecosystems. Isolates of L. lactis and E. faecium did not produce physiologically relevant concentrations of the phyto-hormone indolacetic acid. All isolates solubilized high amount of phosphate and 12 of 16 strains solubilized potassium. E. faecium LB5, L. lactis LB6, LB7, and LB9 inhibited the plant pathogenic Fusarium graminearum to the same extent as two strains of Bacillus sp. However, the antifungal activity of the abovementioned LAB strains depended on the medium of cultivation and a low pH while antifungal activity of Bacillus spp. was independent of the growth medium and likely relates to antifungal lipopeptides. This study showed the potential of rhizospheric LAB for future application as biofertilizers in agriculture.