bluuebunny/crossref_metadata_2025 huggingface dataset:
A retrospective study on the association of gastrointestinal symptoms in children with low lactase activity and low activity of other disaccharidases
Authors: Wasuwanich Paul , Choudry Hassan , Ingviya Thammasin , Scheimann Ann O. , AuYeung Karla J. , Karwowski Christine , Billet Susan , Nichols Buford L. , Karnsakul Wikrom
Published: October 2020

Background

Disaccharides such as lactose and sucrose are sugars commonly found in human diet. They are broken down by mucosal disaccharidases in the duodenum. Previous small studies found no associations between gastrointestinal (GI) symptoms and combined low disaccharidase activity. We aim to explore the associations of low activity of disaccharidase and combinations of low activity of different disaccharidases with general GI symptom presentations in a large cohort of pediatric patients.

Methods

We examined a cohort (0–21 yrs.) who have undergone esophagogastroduodenoscopy and received disaccharidase activity assay from duodenal biopsy in the time period 2010 to 2012. Disaccharidase assays tested for activity of lactase, sucrase, maltase, and palatinase. GI symptoms were grouped into four categories, abdominal pain, diarrhea, weight loss, and gastroesophageal reflux.

Results

Of the 347 subjects, we found an association between low lactase activity and abdominal pain (OR = 1.78; 95% CI = 1.07–2.97; p < 0.05). Subjects with a lactase/sucrase ratio < 0.2 were found to be associated with abdominal pain (OR = 2.25; 95% CI = 1.25–4.04; p < 0.05), Subjects with low pandisaccharidase may be correlated with abdominal pain and have a unique frequency of GI symptoms due to low frequency of diarrhea and weight loss, but they were not statistically significant.

Conclusions

Low activities of certain disaccharidase combinations may be associated with GI symptoms in subjects; a prospective study may be needed to investigate further.
Implications of COVID-19 in high burden countries for HIV/TB: A systematic review of evidence
Authors: Tamuzi Jacques L. , Ayele Birhanu T. , Shumba Constance S. , Adetokunboh Olatunji O. , Uwimana-Nicol Jeannine , Haile Zelalem T. , Inugu Joseph , Nyasulu Peter S.
Published: October 2020

Background

The triple burden of COVID-19, tuberculosis and human immunodeficiency virus is one of the major global health challenges of the twenty-first century. In high burden HIV/TB countries, the spread of COVID-19 among people living with HIV is a well-founded concern. A thorough understanding of HIV/TB and COVID-19 pandemics is important as the three diseases interact. This may clarify HIV/TB/COVID-19 as a newly related field. However, several gaps remain in the knowledge of the burden of COVID-19 on patients with TB and HIV. This study was conducted to review different studies on SARS-CoV, MERS-CoV or COVID-19 associated with HIV/TB co-infection or only TB, to understand the interactions between HIV, TB and COVID-19 and its implications on the burden of the COVID-19 among HIV/TB co-infected or TB patients, screening algorithm and clinical management.

Methods

We conducted an electronic search of potentially eligible studies published in English in the Cochrane Controlled Register of Trials, PubMed, Medrxiv, Google scholar and Clinical Trials Registry databases. We included case studies, case series and observational studies published between January, 2002 and July, 2020 in which SARS-CoV, MERS-CoV and COVID-19 co-infected to HIV/TB or TB in adults. We screened titles, abstracts and full articles for eligibility. Descriptive and meta-analysis were done and results have been presented in graphs and tables.

Results

After removing 95 duplicates, 58 out of 437 articles were assessed for eligibility, of which 14 studies were included for descriptive analysis and seven studies were included in the meta-analysis. Compared to the descriptive analysis, the meta-analysis showed strong evidence that current TB exposure was high-risk COVID-19 group (OR 1.67, 95% CI 1.06–2.65, P = 0.03). The pooled of COVID-19/TB severity rate increased from OR 4.50 (95% CI 1.12–18.10, P = 0.03), the recovery rate was high among COVID-19 compared to COVID-19/TB irrespective of HIV status (OR 2.23, 95% CI 1.83–2.74, P < 0.001) and the mortality was reduced among non-TB group (P < 0.001).

Conclusion

In summary, TB was a risk factor for COVID-19 both in terms of severity and mortality irrespective of HIV status. Structured diagnostic algorithms and clinical management are suggested to improve COVID-19/HIV/TB or COVID-19/TB co-infections outcomes.
Prevalence and diversity of enteric pathogens among cholera treatment centre patients with acute diarrhea in Uvira, Democratic Republic of Congo
Authors: Williams Camille , Cumming Oliver , Grignard Lynn , Rumedeka Baron Bashige , Saidi Jaime Mufitini , Grint Daniel , Drakeley Chris , Jeandron Aurelie
Published: October 2020

Background

Cholera remains a major global health challenge. Uvira, in the Democratic Republic of the Congo (DRC), has had endemic cholera since the 1970’s and has been implicated as a possible point of origin for national outbreaks. A previous study among this population, reported a case confirmation rate of 40% by rapid diagnostic test (RDT) among patients at the Uvira Cholera Treatment Centre (CTC). This study considers the prevalence and diversity of 15 enteric pathogens in suspected cholera cases seeking treatment at the Uvira CTC.

Methods

We used the Luminex xTAG® multiplex PCR to test for 15 enteric pathogens, including toxigenic strains of V. cholerae in rectal swabs preserved on Whatman FTA Elute cards. Results were interpreted on MAGPIX® and analyzed on the xTAG® Data Analysis Software. Prevalence of enteric pathogens were calculated and pathogen diversity was modelled with a Poisson regression.

Results

Among 269 enrolled CTC patients, PCR detected the presence of toxigenic Vibrio cholerae in 38% (103/269) of the patients, which were considered to be cholera cases. These strains were detected as the sole pathogen in 36% (37/103) of these cases. Almost half (45%) of all study participants carried multiple enteric pathogens (two or more). Enterotoxigenic Escherichia coli (36%) and Cryptosporidium (28%) were the other most common pathogens identified amongst all participants. No pathogen was detected in 16.4% of study participants. Mean number of pathogens was highest amongst boys and girls aged 1–15 years and lowest in women aged 16–81 years. Ninety-three percent of toxigenic V. cholerae strains detected by PCR were found in patients having tested positive for V. cholerae O1 by RDT.

Conclusions

Our study supports previous results from DRC and other cholera endemic areas in sub-Sahara Africa with less than half of CTC admissions positive for cholera by PCR. More research is required to determine the causes of severe acute diarrhea in these low-resource, endemic areas to optimize treatment measures.

Trial registration

This study is part of the impact evaluation study entitled: “Impact Evaluation of Urban Water Supply Improvements on Cholera and Other Diarrheal Diseases in Uvira, Democratic Republic of Congo” registered on 10 October 2016 at clinicaltrials.gov Identification number: NCT02928341.
Genetic variants in MIR17HG affect the susceptibility and prognosis of glioma in a Chinese Han population
Authors: Feng Jigao , Ouyang Yibin , Xu Dedong , He Qinglong , Liu Dayuan , Fan Xudong , Xu Pengxiang , Mo Yehe
Published: October 2020

Background

lncRNA MIR17HG was upregulated in glioma, and participated in promoting proliferation, migration and invasion of glioma. However, the role of MIR17HG polymorphisms in the occurrence and prognosis of glioma is still unclear.

Methods

In the study, 592 glioma patients and 502 control subjects were recruited. Agena MassARRAY platform was used to detect the genotype of MIR17HG polymorphisms. Logistic regression analysis was used to evaluate the relationship between MIR17HG single nucleotide polymorphisms (SNPs) and glioma risk by odds ratio (OR) and 95% confidence intervals (CIs). Kaplan–Meier curves, Cox hazards models were performed for assessing the role of these SNPs in glioma prognosis by hazard ratios (HR) and 95% CIs.

Results

We found that rs7318578 (OR = 2.25, p = 3.18 × 10− 5) was significantly associated with glioma susceptibility in the overall participants. In the subgroup with age <  40 years, rs17735387 (OR = 1.53, p = 9.05 × 10− 3) and rs7336610 (OR = 1.35, p = 0.016) were related to the higher glioma susceptibility. More importantly, rs17735387 (HR = 0.82, log-rank p = 0.026) were associated with the longer survival of glioma patients. The GA genotype of rs17735387 had a better overall survival (HR = 0.75, log-rank p = 0.013) and progression free survival (HR = 0.73, log-rank p = 0.032) in patients with I-II glioma. We also found that rs72640334 was related to the poor prognosis (HR = 1.49, Log-rank p = 0.035) in female patients. In the subgroup of patients with age ≥ 40 years, rs17735387 was associated with a better prognosis (HR = 0.036, Log-rank p = 0.002).

Conclusion

Our study firstly reported that MIR17HG rs7318578 was a risk factor for glioma susceptibility and rs17735387 was associated with the longer survival of glioma among Chinese Han population, which might help to enhance the understanding of MIR17HG gene in gliomagenesis. In subsequent studies, we will continue to collect samples and follow up to further validate our findings and further explore the function of these MIR17HG SNPs in glioma in a larger sample size.
Repetitive negative thinking is associated with subjective cognitive decline in older adults: a cross-sectional study
Authors: Schlosser Marco , Demnitz-King Harriet , Whitfield Tim , Wirth Miranka , Marchant Natalie L.
Published: October 2020

Background

In the absence of a cure or effective treatment for dementia, attention has shifted towards identifying risk factors for prevention. Subjective Cognitive Decline (SCD) describes self-perceived worsening of cognition despite unimpaired performance on neuropsychological tests. SCD has been associated with an increased dementia risk and steeper memory decline. Repetitive negative thinking (RNT) is a transdiagnostic process that manifests across several mental health disorders associated with increased vulnerability to dementia. RNT has thus been proposed as a candidate marker of risk for dementia and, relatedly, could contribute to the manifestation of SCD. We aimed to investigate the relationship between SCD and RNT alongside other proposed psychological risk/protective factors for dementia and cognitive decline.

Methods

In a cross-sectional online survey, 491 older adults (mean = 64.9 years,SD = 4.2; 63.1% female) completed measures of RNT, personality traits, purpose in life, worry, rumination, and meditation practice. SCD was assessed continuously via self-perceived cognitive function (Neuro-QoL) and categorically via endorsement (yes/no) of memory complaints. Regression models, using a stepwise backwards elimination, were built to assess associations between SCD, demographics, and all risk/protective factors.

Results

A total of 24.2% of participants reported memory complaints. In the final prediction models, RNT was the only psychological variable associated with lower self-perceived cognitive function and with a higher likelihood of memory complaints.

Conclusions

This study empirically corroborates the theoretical relationship between SCD and RNT. Longitudinal studies are needed to establish whether RNT is a prodromal symptom or an independent risk factor, and whether RNT can be a promising construct for future research on SCD and dementia risk.
Exploring attitudes of adolescents and caregivers towards community-based delivery of the HPV vaccine: a qualitative study
Authors: Ramanadhan Shoba , Fontanet Constance , Teixeira Marina , Mahtani Sitara , Katz Ingrid
Published: October 2020

Background

Human Papillomavirus (HPV) vaccination among adolescents is an important strategy to prevent cervical and other cancers in adulthood. However, uptake remains far below the Healthy People 2020 targets for the US. Given the barriers to population-level vaccination policies and challenges to incorporating additional action items during clinical visits, we sought to explore alternative delivery mechanisms, specifically delivery of the vaccine in community settings.

Methods

We conducted six focus groups (three with adolescents aged 11–14 who had not received the HPV vaccine and three with caregivers of adolescents meeting those criteria) from Black, Latino, and Brazilian communities in Massachusetts. We utilized a framework analysis approach that involved a multi-stage coding process employing both prefigured and emergent codes. Initial interpretations were refined through consultation with an advisory board.

Results

Adolescents and caregivers expressed a range of concerns about the HPV vaccine and also described interest in learning more about the vaccine, emphasizing the importance of a relationship with a trusted provider as a facilitator of vaccine acceptance. Regarding community-based delivery of the vaccine, reactions were mainly negative. However, adolescents and caregivers noted that receiving information in community settings that could seed a conversation with a trusted provider would be welcome. Interestingly, the notion of a trusted provider seemed to extend broadly to practitioners linked to the trusted main provider.

Conclusions

The study highlights an opportunity for increasing HPV vaccination among some racial and ethnic minority populations by leveraging trusted community organizations to provide information and seed conversations with a potentially broad group of trusted providers. A task-shifting approach, or reliance on staff with fewer formal credentials, may offer opportunities to support vaccination in resource-constrained settings.
Students’ and lecturers’ perspective on the implementation of online learning in dental education due to SARS-CoV-2 (COVID-19): a cross-sectional study
Authors: Schlenz Maximiliane Amelie , Schmidt Alexander , Wöstmann Bernd , Krämer Nobert , Schulz-Weidner Nelly
Published: October 2020

Background

On account of physical distancing measures, universities worldwide are strongly affected by SARS-CoV-2 (COVID-19). Thus, the dental school of Justus-Liebig-University Giessen (Germany) transferred the established “face-to-face” learning to online learning in the spring term 2020. The aim of this study was to assess the students’ and lecturers’ perspectives on the implementation of online learning due to COVID-19, using a questionnaire survey.

Methods

After the online period, all students and lecturers were asked to fill out an online questionnaire containing evaluative statements regarding handling, didactic benefit, motivation, and overall assessment. Furthermore, the questionnaire for lecturers contained additional aspects regarding knowledge gain in terms of providing online learning. Besides that, students and lecturers were asked for the amount of online learning in the future curriculum (independent of COVID-19). Data were subjected to regression analysis and T-test (p < .05).

Results

36.8% of students preferred “face-to-face” learning instead of sole online learning. An increase of know how concerning online teaching was observable for lecturers. Both, students and lecturers, want to keep up with online courses in the future curriculum. However, in terms of the optimal amount of online learning a significant difference between students’ and lecturers’ perspective was observed. While students suggested 53.2% (24.9) (mean (standard deviation)) lecturers only stated 38.6% (21.5).

Conclusions

Within the limitation of this study, students’ and lecturers’ showed a predominantly positive perspective on the implementation of online learning, providing the chance to use online learning even beyond COVID-19 in the future curriculum.
Poor level of knowledge on elderly care despite positive attitude among nursing students in Zanzibar Island: findings from a cross-sectional study
Authors: Muhsin Arafa A. , Munyogwa Mariam J. , Kibusi Stephen M. , Seif Saada A.
Published: October 2020

Background

It is estimated by the year 2050, 80% of the global elderly population will be from the low-and middle income countries. Elderly care requires health workers with skills associated with an understanding of the biological, psychological, social and cultural theories related to aging. Nurses with better knowledge, skills and positive attitudes towards elderly care are highly needed and critically important for better healthcare and wellbeing of the elderly population. Therefore the objective of this study was to assess the level of knowledge and attitude of nursing students towards elderly care in Zanzibar Island.

Methods

A cross-sectional study was conducted in Zanzibar involving three out of five nursing training institutions. Participants were selected by systematic random sampling. Facts on Aging Quiz 2 and Kogan’s Attitudes Toward Old People scale were used to assess the level of knowledge and attitude towards elderly care among the students respectively. Simple and multivariable logistic regressions were applied to determine the predictors of knowledge and attitude among the participants.

Results

A total of 393 students participated in this study. Only 17% (69) of the participants had good level of knowledge and about 67.9% (267) had positive attitude towards elderly care. Living in an extended family and with an elderly person at home were both associated with good level of knowledge and positive attitude towards elderly care. Furthermore, living in a rural area (adjusted odds ratio = 2.23; 95% confidence interval: 1.22, 4.10) and studying at public institution (adjusted odds ratio = 2.59; 95% confidence interval: 1.41, 4.63) were associated with positive attitude towards elderly care.

Conclusion

This study has shown that the majority of nursing students in Zanzibar have positive attitude but poor level of knowledge towards elderly care. The current findings have demonstrated that past experience with an elderly person can help in influencing good knowledge and shaping positive attitudes towards elderly care. Low level of knowledge shown in the study suggests for further research on adequacy of nursing curriculum and/or its implementation.
Use of the ankle-brachial index combined with the percentage of mean arterial pressure at the ankle to improve prediction of all-cause mortality in type 2 diabetes mellitus: an observational study
Authors: Li Yu-Hsuan , Sheu Wayne Huey-Herng , Lee I-Te
Published: October 2020

Background

Peripheral artery disease (PAD) in the lower extremities is a common complication of type 2 diabetes and has been shown to be associated with mortality. The ankle-brachial index (ABI) is a simple noninvasive method to screen PAD, but this method has limited sensitivity. We hypothesized that using the percentage of mean arterial pressure (%MAP) in combination with the ABI would improve the prediction of mortality.

Methods

We retrospectively collected data from patients with type 2 diabetes who had undergone ABI and  %MAP measurements at our hospital. We separated the cohort into four groups according to their ABI and  %MAP values, and we examined whether these indices were associated with mortality.

Results

A total of 5569 patients (mean age, 65 ± 11 years) were enrolled. During the follow-up period (median, 22.9 months), 266 (4.8%) of the enrolled patients died. The combination of ABI and  %MAP was significantly more effective than ABI alone for predicting mortality (C index of 0.62, 95% confidence interval [CI] of 0.57 to 0.65 vs. C index of 0.57, 95% CI of 0.53 to 0.62; P = 0.038). In multivariate analysis (with a reference group defined by ABI > 0.90 and  %MAP ≤ 45%), the highest risk of mortality was seen in patients with ABI ≤ 0.90 and  %MAP > 45% (hazard ratio = 2.045 [95% CI 1.420, 2.945], P < 0.001).

Conclusions

The use of  %MAP alongside ABI appears to significantly improve the prediction of all-cause mortality in patients with type 2 diabetes.
Comparison of leucocyte profiles between healthy children and those with asymptomatic and symptomatic Plasmodium falciparum infections
Authors: Prah Diana Ahu , Amoah Linda Eva , Gibbins Matthew P. , Bediako Yaw , Cunnington Aubrey J. , Awandare Gordon A. , Hafalla Julius Clemence R.
Published: October 2020

Background

The immune mechanisms that determine whether aPlasmodium falciparuminfection would be symptomatic or asymptomatic are not fully understood. Several studies have been carried out to characterize the associations between disease outcomes and leucocyte numbers. However, the majority of these studies have been conducted in adults with acute uncomplicated malaria, despite children being the most vulnerable group.

Methods

Peripheral blood leucocyte subpopulations were characterized in children with acute uncomplicated (symptomatic; n = 25) or asymptomatic (n = 67)P. falciparummalaria, as well as malaria-free (uninfected) children (n = 16) from Obom, a sub-district of Accra, Ghana. Leucocyte subpopulations were enumerated by flow cytometry and correlated with two measures of parasite load: (a) plasma levels ofP. falciparumhistidine-rich protein 2 (PfHRP2) as a proxy for parasite biomass and (b) peripheral blood parasite densities determined by microscopy.

Results

In children with symptomaticP. falciparuminfections, the proportions and absolute cell counts of total (CD3 +) T cells, CD4 + T cells, CD8 + T cells, CD19 + B cells and CD11c + dendritic cells (DCs) were significantly lower as compared to asymptomaticP. falciparum-infected and uninfected children. Notably, CD15 + neutrophil proportions and cell counts were significantly increased in symptomatic children. There was no significant difference in the proportions and absolute counts of CD14 + monocytes amongst the three study groups. As expected, measures of parasite load were significantly higher in symptomatic cases. Remarkably,PfHRP2 levels and parasite densities negatively correlated with both the proportions and absolute numbers of peripheral leucocyte subsets: CD3 + T, CD4 + T, CD8 + T, CD19 + B, CD56 + NK, γδ + T and CD11c + cells. In contrast, bothPfHRP2 levels and parasite densities positively correlated with the proportions and absolute numbers of CD15 + cells.

Conclusions

SymptomaticP. falciparuminfection is correlated with an increase in the levels of peripheral blood neutrophils, indicating a role for this cell type in disease pathogenesis. Parasite load is a key determinant of peripheral cell numbers during malaria infections.
The effects of step-count monitoring interventions on physical activity: systematic review and meta-analysis of community-based randomised controlled trials in adults
Authors: Chaudhry Umar A. R. , Wahlich Charlotte , Fortescue Rebecca , Cook Derek G. , Knightly Rachel , Harris Tess
Published: October 2020

Background

Step-count monitors (pedometers, body-worn trackers and smartphone applications) can increase walking, helping to tackle physical inactivity. We aimed to assess the effect of step-count monitors on physical activity (PA) in randomised controlled trials (RCTs) amongst community-dwelling adults; including longer-term effects, differences between step-count monitors, and between intervention components.

Methods

Systematic literature searches in seven databases identified RCTs in healthy adults, or those at risk of disease, published between January 2000–April 2020. Two reviewers independently selected studies, extracted data and assessed risk of bias. Outcome was mean differences (MD) with 95% confidence intervals (CI) in steps at follow-up between treatment and control groups. Our preferred outcome measure was from studies with follow-up steps adjusted for baseline steps (change studies); but we also included studies reporting follow-up differences only (end-point studies). Multivariate-meta-analysis used random-effect estimates at different time-points for change studies only. Meta-regression compared effects of different step-count monitors and intervention components amongst all studies at ≤4 months.

Results

Of 12,491 records identified, 70 RCTs (at generally low risk of bias) were included, with 57 trials (16,355 participants) included in meta-analyses: 32 provided change from baseline data; 25 provided end-point only. Multivariate meta-analysis of the 32 change studies demonstrated step-counts favoured intervention groups: MD of 1126 steps/day 95%CI [787, 1466] at ≤4 months, 1050 steps/day [602, 1498] at 6 months, 464 steps/day [301, 626] at 1 year, 121 steps/day [− 64, 306] at 2 years and 434 steps/day [191, 676] at 3–4 years. Meta-regression of the 57 trials at ≤4 months demonstrated in mutually-adjusted analyses that: end-point were similar to change studies (+ 257 steps/day [− 417, 931]); body-worn trackers/smartphone applications were less effective than pedometers (− 834 steps/day [− 1542, − 126]); and interventions providing additional counselling/incentives were not better than those without (− 812 steps/day [− 1503, − 122]).

Conclusions

Step-count monitoring leads to short and long-term step-count increases, with no evidence that either body-worn trackers/smartphone applications, or additional counselling/incentives offer further benefit over simpler pedometer-based interventions. Simple step-count monitoring interventions should be prioritised to address the public health physical inactivity challenge.

Systematic review registration

PROSPERO numberCRD42017075810.
First detection and molecular characterisation of pseudocowpox virus in a cattle herd in Zambia
Authors: Ziba Maureen Wakwamba , Chitala Chanda , Settypalli Tirumala Bharani K. , Mumba Malama , Cattoli Giovanni , Fandamu Paul , Lamien Charles Euloge
Published: October 2020

Background

Pseudocowpox virus (PCPV) of the genus Parapoxvirus in the family Poxviridae causes pseudocowpox in cattle worldwide and presents a zoonotic concern. Most poxviruses produce diseases of similar clinical signs in affected animals, which are impossible to differentiate clinically or by serology. It is, therefore, vital to use molecular assays to rapidly identify the causative agents of poxvirus infections. This study aimed to detect, diagnose, and characterize the causative agent of pox-like skin lesions in a cattle herd in Zambia, initially suspected to be infected with Lumpy Skin Disease virus.

Methods

We used a High-Resolution Melting (HRM) analysis assay to detect the PCPV genome and sequenced the major envelope protein (B2L gene) for comparative sequence and phylogenetic analysis.

Results

Our field investigations showed cattle presenting atypical skin lesions and high morbidity within the herd. The laboratory diagnosis, based on the HRM assay revealed PCPV DNA in the samples. Phylogenetic and comparative sequence analyses confirmed PCPV in the samples and revealed genomic differences between samples collected in 2017 and 2018 from the same farm.

Conclusion

Our work is the first documented report of PCPV in Zambia. It shows the strength of molecular methods to diagnose pox-like infections in cattle and discriminate between diseases causing similar clinical signs. This rapid and accurate diagnosis improves the response time for more accurate veterinary interventions.
RETRACTED ARTICLE: The effect of early oral stimulation with breast milk on the feeding behavior of infants after congenital cardiac surgery
Authors: Yu Xian-Rong , Huang Shu-Ting , Xu Ning , Wang Li-Wen , Wang Zeng-Chun , Cao Hua , Chen Qiang
Published: October 2020

Objective

To investigate the effect of early oral stimulation with breast milk on the feeding behavior of infants after congenital cardiac surgery.

Methods

Infants with congenital heart disease were randomly divided into the breast milk oral stimulation group (n = 23), physiological saline oral stimulation group (n = 23) and control group (n = 23). Debra Beckman’s oral exercise program was used with breast milk and physiological saline in the breast milk oral stimulation group and the physiological saline oral stimulation group, respectively. The time oral feeding and total oral nutrition were started, the length of intensive care unit (ICU) stay and hospital stay, weight and the complications at discharge were recorded for each group and statistically analyzed.

Results

The time oral feeding and total oral nutrition were started and the length of ICU stay and hospital stay were significantly less in the breast milk oral stimulation group and physiological saline oral stimulation group than in the control group (P < 0.05). There were no significant differences in other indicators between the breast milk oral stimulation group and the physiological saline oral stimulation group, except for the time total oral nutrition began (P < 0.05). However, there were no significant differences in weight or complications at discharge among the three groups (P > 0.05).

Conclusion

Early oral stimulation exercises with breast milk can help infant patients quickly recover total oral nutrition and reduce the length of ICU and hospital stay after cardiac surgery.
Factor XII in PMM2-CDG patients: role of N-glycosylation in the secretion and function of the first element of the contact pathway
Authors: López-Gálvez Raquel , de la Morena-Barrio María Eugenia , López-Lera Alberto , Pathak Monika , Miñano Antonia , Serrano Mercedes , Borgel Delphine , Roldán Vanessa , Vicente Vicente , Emsley Jonas , Corral Javier
Published: October 2020

Background

Congenital disorders of glycosylation (CDG) are rare diseases with impaired glycosylation and multiorgan disfunction, including hemostatic and inflammatory disorders. Factor XII (FXII), the first element of the contact phase, has an emerging role in hemostasia and inflammation. FXII deficiency protects against thrombosis and the p.Thr309Lys variant is involved in hereditary angioedema through the hyperreactivity caused by the associated defective O-glycosylation. We studied FXII in CDG aiming to supply further information of the glycosylation of this molecule, and its functional and clinical effects. Plasma FXII from 46 PMM2-CDG patients was evaluated by coagulometric and by Western Blot in basal conditions, treated with N-glycosydase F or activated by silica or dextran sulfate. A recombinant FXII expression model was used to validate the secretion and glycosylation of wild-type and variants targeting the two described FXII N-glycosylation sites (p.Asn230Lys; p.Asn414Lys) as well as the p.Thr309Lys variant.

Results

PMM2-CDG patients had normal FXII levels (117%) but high proportions of a form lacking N-glycosylation at Asn414. Recombinant FXII p.Asn230Lys, and p.Asn230Lys&p.Asn414Lys had impaired secretion and increased intracellular retention compared to wild-type, p.Thr309Lys and p.Asn414Lys variants. The hypoglycosylated form of PMM2-CDG activated similarly than FXII fully glycosylated. Accordingly, no PMM2-CDG had angioedema. FXII levels did not associate to vascular events, but hypoglycosylated FXII, like hypoglycosylated transferrin, antithrombin and FXI levels did it.

Conclusions

N-glycosylation at Asn230 is essential for FXII secretion. PMM2-CDG have high levels of FXII lacking N-glycosylation at Asn414, but this glycoform displays similar activation than fully glycosylated, explaining the absence of angioedema in CDG.
High-throughput approaches for precision medicine in high-grade serous ovarian cancer
Authors: Govindarajan Meinusha , Wohlmuth Christoph , Waas Matthew , Bernardini Marcus Q. , Kislinger Thomas
Published: October 2020

High-grade serous carcinoma (HGSC) is the most prevalent and aggressive subtype of ovarian cancer. The large degree of clinical heterogeneity within HGSC has justified deviations from the traditional one-size-fits-all clinical management approach. However, the majority of HGSC patients still relapse with chemo-resistant cancer and eventually succumb to their disease, evidence that further work is needed to improve patient outcomes. Advancements in high-throughput technologies have enabled novel insights into biological complexity, offering a large potential for informing precision medicine efforts. Here, we review the current landscape of clinical management for HGSC and highlight applications of high-throughput biological approaches for molecular subtyping and the discovery of putative blood-based biomarkers and novel therapeutic targets. Additionally, we present recent improvements in model systems and discuss how their intersection with high-throughput platforms and technological advancements is positioned to accelerate the realization of precision medicine in HGSC.
Maximum common property: a new approach for molecular similarity
Authors: Antelo-Collado Aurelio , Carrasco-Velar Ramón , García-Pedrajas Nicolás , Cerruela-García Gonzalo
Published: October 2020

The maximum common property similarity (MCPhd) method is presented using descriptors as a new approach to determine the similarity between two chemical compounds or molecular graphs. This method uses the concept of maximum common property arising from the concept of maximum common substructure and is based on the electrotopographic state index for atoms. A new algorithm to quantify the similarity values of chemical structures based on the presented maximum common property concept is also developed in this paper. To verify the validity of this approach, the similarity of a sample of compounds with antimalarial activity is calculated and compared with the results obtained by four different similarity methods: the small molecule subgraph detector (SMSD), molecular fingerprint based (OBabel_FP2), ISIDA descriptors and shape-feature similarity (SHAFTS). The results obtained by the MCPhd method differ significantly from those obtained by the compared methods, improving the quantification of the similarity. A major advantage of the proposed method is that it helps to understand the analogy or proximity between physicochemical properties of the molecular fragments or subgraphs compared with the biological response or biological activity. In this new approach, more than one property can be potentially used. The method can be considered a hybrid procedure because it combines descriptor and the fragment approaches.
Influence of X-ray computed tomography (CT) exposure and reconstruction parameters on positron emission tomography (PET) quantitation
Authors: Ho Shon Ivan , Reece Christopher , Hennessy Thomas , Horsfield Megan , McBride Bruce
Published: October 2020

Background

The CT of PET CT provides diagnostic information, anatomic localisation and attenuation correction (AC). When only AC is required, very lose dose CT is desirable. CT iterative reconstruction (IR) improves image quality with lower exposures however there is little data on very low dose IR CT for AC of PET. This work assesses the impact of CT exposure and reconstruction algorithm on PET voxel values.

Method

An anthropomorphic torso phantom was filled with physiologically typical [18]F concentrations in heart, liver and background compartments. A 17-mm-diameter right lung “tumour” filled with [18]F was included (surrounding lung contained no 18[F]). PET was acquired followed by 24 CT acquisitions with varying CT exposures (15–50 mAs, 80–120 kVp, pitch 0.671 or 0.828). Each CT was reconstructed twice using filtered back projection (FBP) or IR and these used for AC of PET. The reference PET reconstruction (RR) used CT acquired at 50 mAs, 120 kVp, pitch 0.828, IR, all others were test PET reconstructions (TR). Regions of interest (ROIs) were drawn in the liver, soft tissue and over “tumour” on each TR and compared with the RR. Voxel values in each TR were compared to the RR using a paired t test and by calculating which and what proportion of voxels in each TR differed by a quantitatively significant difference (QSD) from the RR.

Results

TRs reconstructed using lower dose CTs underestimated mean and maximum ROI activity relative to the RR; greater with IR than FBP. Once CT dose index (CTDI) increased to 1 mGy, differences were less than QSD. On voxel analysis, all TRs were significantly different to the RR (p < 0.0001). TRs reconstructed at the lowest CT exposure with IR had 6% of voxels that differed by greater than QSD. Differences were reduced with increasing CTDI and FBP reconstruction. Voxels which exceeded the QSD were spatially localised to regions of high activity, interfaces between different attenuation and areas of CT beam hardening.

Conclusions

Very low dose CT exposures are feasible for accurate PET AC. Scanner- and reconstruction-specific validation should be employed prior very low dose CT AC for PET.
Faith-based organizations: humanitarian mission or religious missionary
Authors: Khafagy Riham Ahmed
Published: October 2020

This paper analyzes Islamic FBOs’ humanitarian approaches, programs, and challenges. Politicalized religious interpretations are also on board to investigate their missionary aspects. I design my argument based on Michael Barnett and Janice Grass Stein’s assumption on the impact of social constructions on establishing sacred and secular concepts and spaces. Thus, I study the UK-based Islamic Relief Worldwide (IRW) and the Kuwaiti Direct Aid Society (DAS) to examine the influence of their social settings on their humanitarian experiences. My question is “do different social settings shape various humanitarian approach, although of sharing the same religious mission?” I argue that Islamic rules encourage Muslims to be religiously committed to paying charity and showing human and religious solidarity. In this regard, flexible Islamic Fiqh (jurisprudence) allows Muslima to set various socially constructed implementations of these religious commitments. Humanitarian relief is not an exception in this regard. Therefore, Islamic FBOs lay down on a continuum based on their socially constructed models which reflect different interpretations of religious texts and their applications to understanding societal issues as well as various employed strategies of these civil society actors.
An analysis of movement patterns in mass casualty incident simulations
Authors: Tolg Boris , Lorenz Juergen
Published: October 2020

Background

Mass casualty incidents (MCI) such as train or bus crashes, explosions, collapses of buildings, or terrorist attacks result in rescue teams facing many victims and in huge challenges for hospitals. Simulations are performed to optimize preparedness for MCI. To maximize the benefits of MCI simulations, it is important to collect large amounts of information. However, a clear concept and standardization of a data-driven post-exercise evaluation and debriefing are currently lacking.

Methods

GPS data loggers were used to track the trajectories of patients, medics, and paramedics in two simulated MCI scenarios using real human actors. The distribution of patients over the treatment area and their time of arrival at the hospital were estimated to provide information on the quality of triage and for debriefing purposes.

Results

The results show the order in which patients have been treated and the time for the individual arrivals as an indicator for the triage performance. The distribution of patients at the accident area suggested initial confusion and unclear orders for the placement of patients with different grades of injury that can be used for post-exercise debriefing. The dynamics of movement directions allowed to detect group behavior during different phases of the MCI.

Conclusions

Results indicate that GPS data loggers can be used to collect precise information about the trajectories of patients and rescue teams at an MCI simulation without interfering with the realism of the simulation. The exact sequence of the deliverance of patients of different triage categories to their appropriate destinations can be used to evaluate team performance for post-exercise debriefing. Future MCI simulations are planned to validate the use of GPS loggers by providing “hot-debrief” immediately after the MCI simulation and to explore ways in which group detection can provide relevant information for post-exercise evaluations

Trial registration

Not applicable.
Multiobjective Bayesian optimization and joint inversion for active sensor fusion
Authors: Haan Sebastian , Ramos Fabio , Dietmar Müller R.
Published: October 2020

A critical decision process in data acquisition for mineral and energy resource exploration is how to efficiently combine a variety of sensor types and how to minimize the total cost. We have developed a probabilistic framework for multiobjective optimization and inverse problems given an expensive cost function for allocating new measurements. This new method is devised to jointly solve multilinear forward models of 2D sensor data and 3D geophysical properties using sparse Gaussian process kernels while taking into account the cross-variances of different parameters. Multiple optimization strategies are tested and evaluated on a set of synthetic and real geophysical data. We determine the advantages on a specific example of a joint inverse problem, recommending where to place new drill-core measurements given 2D gravity and magnetic sensor data; the same approach can be applied to a variety of remote sensing problems with linear forward models — ranging from constraints limiting surface access for data acquisition to adaptive multisensor positioning.
Prestack seismic facies analysis via waveform sparse representations
Authors: Li Kunhong , Liu Zhining , She Bin , Liang Jiandong , Hu Guangmin
Published: October 2020

Seismic facies analysis based on prestack data is becoming popular. Vertical elastic transitions produce the spatial structure variation of prestack waveforms, whereas lateral elastic transitions produce the amplitude intensity variation. In stratigraphic seismic facies analysis, more attention should be paid to waveform spatial structure than amplitude intensity. Conventional classification methods based on distance metrics are difficult to adapt to stratigraphic seismic facies analysis because a distance metric is a comprehensive measure of waveform structure and amplitude intensity. We have developed a dictionary learning (DL) method for prestack seismic facies analysis. The method first learns several dictionaries from labeled prestack waveform data, and these dictionaries consist of several normalization vector bases. The prestack waveform spatial structure is, therefore, embedded in these learned dictionaries, and the amplitude intensity is eliminated by the normalization process. Afterward, these dictionaries are used to sparsely represent prestack seismic data. Seismic facies are classified and determined according to representation error. A source error separation method is used to improve the antinoise performance of DL by iteratively segmenting the noise out in the training data. The results on synthetic and field seismic data show that the proposed method has a stronger tolerance to noise than K singular value decomposition, and the obtained seismic facies boundary is more accurate and clearer than the support vector machine and k-nearest neighbors algorithm. This demonstrates that the proposed method is an effective seismic facies analysis technique.
Magnetic amplitude inversion for depth-to-basement and apparent magnetization-intensity estimates
Authors: Hidalgo-Gato Marlon C. , Barbosa Valéria C. F. , Oliveira Vanderlei C.
Published: October 2020

We have developed an inversion method to recover the depth and the total magnetization intensity of the basement under a sedimentary basin using the amplitude of the magnetic anomaly vector (amplitude data). Because the amplitude data are weakly dependent on the magnetization direction, our method is suitable for interpreting areas with remanent magnetization. Our method assumes constant magnetized basement rocks overlain by nonmagnetic sediments. To overcome the inherent ambiguity of potential field data, we assume knowledge of the average depth of the basement and use it as a constraint to regularize the inversion. A sensitivity analysis with synthetic data shows the weak dependency of the magnetic amplitude inversion on the magnetization direction. Different combinations of magnetization directions recover the interface separating sediments from basement rocks. Test on field data over the Foz do Amazonas Basin, Brazil, recovers the shape of the basement relief without any knowledge about the magnetization intensity and direction. The estimated basement relief reveals a smooth basement framework with basement highs in the central part of the area. In a regional-scale perspective, the deeper and constant estimated basement relief at the northernmost limit of the area may suggest changing in crustal domains from a hyperextended continental crust to homogeneous oceanic crust.
Anderson acceleration for seismic inversion
Authors: Yang Yunan
Published: October 2020

State-of-the-art seismic imaging techniques treat inversion tasks such as full-waveform inversion (FWI) and least-squares reverse time migration (LSRTM) as partial differential equation-constrained optimization problems. Due to the large-scale nature, gradient-based optimization algorithms are preferred in practice to update the model iteratively. Higher-order methods converge in fewer iterations but often require higher computational costs, more line-search steps, and bigger memory storage. A balance among these aspects has to be considered. We have conducted an evaluation using Anderson acceleration (AA), a popular strategy to speed up the convergence of fixed-point iterations, to accelerate the steepest-descent algorithm, which we innovatively treat as a fixed-point iteration. Independent of the unknown parameter dimensionality, the computational cost of implementing the method can be reduced to an extremely low dimensional least-squares problem. The cost can be further reduced by a low-rank update. We determine the theoretical connections and the differences between AA and other well-known optimization methods such as L-BFGS and the restarted generalized minimal residual method and compare their computational cost and memory requirements. Numerical examples of FWI and LSRTM applied to the Marmousi benchmark demonstrate the acceleration effects of AA. Compared with the steepest-descent method, AA can achieve faster convergence and can provide competitive results with some quasi-Newton methods, making it an attractive optimization strategy for seismic inversion.
Dread
Authors: James Jennifer C.
Published: October 2020

Reinterpreting nineteenth-century philosopher Søren Kierkegaard’s theory of “dread,” this essay situates the fear surrounding COVID-19 within a larger historical framework to consider the affective dimension of the virus’s emergence for African Americans.
An Ethics Debate for the Ages: American Individualism and the Dilemma of the Healthy Carrier
Authors: Nixon Kari
Published: October 2020

Many public health ethics debates are construed as the rights of the collective versus the rights of the individual. This essay demonstrates that in the context of diseases which are transmitted by healthy carriers, the issue is more complex than this. Instead of arguing about competing rights, this essay argues that such debates are first about competing visions of reality, in which the individual is asked to substitute a collective understanding of their body for their own personal experience of their body. Understanding this first layer of the ethics debate in such healthy carrier situations allows us to redirect persuasive energies, moving away from a beginning-point of compliance to one of understanding, which may ultimately find a more willing public audience.
Who Counts? Old Age in COVID Times
Authors: Thrailkill Jane F.
Published: October 2020

People over sixty-five have been singled out as a uniquely vulnerable risk group for the novel coronavirus. Yet the discourse of risk obscures (and exacerbates) socially created dangers of congregate care in the United States: poorly paid workers holding down multiple jobs and the endemic “plagues” of loneliness, boredom, and hopelessness. Humorous memes about who counts as old point out structural inequalities, while millions of able-bodied “shut-ins” (due to lockdowns and job losses) may experience forced empathy: fuel for new imaginings about how to care for—and value—elders moving forward.