bluuebunny/crossref_metadata_2025 huggingface dataset:
Antibiotic resistance in plant growth promoting bacteria: A comprehensive review and future perspectives to mitigate potential gene invasion risks
Authors: Mahdi Ismail , Fahsi Nidal , Hijri Mohamed , Sobeh Mansour
Published: September 2022

Plant growth-promoting bacteria (PGPB) are endowed with several attributes that can be beneficial for host plants. They opened myriad doors toward green technology approach to reduce the use of chemical inputs, improve soil fertility, and promote plants’ health. However, many of these PGPB harbor antibiotic resistance genes (ARGs). Less attention has been given to multi-resistant bacterial bioinoculants which may transfer their ARGs to native soil microbial communities and other environmental reservoirs including animals, waters, and humans. Therefore, large-scale inoculation of crops by ARGs-harboring bacteria could worsen the evolution and dissemination of antibiotic resistance and aggravate the negative impacts on such ecosystem and ultimately public health. Their introduction into the soil could serve as ARGs invasion which may inter into the food chain. In this review, we underscore the antibiotic resistance of plant-associated bacteria, criticize the lack of consideration for this phenomenon in the screening and application processes, and provide some recommendations as well as a regulation framework relating to the development of bacteria-based biofertilizers to aid maximizing their value and applications in crop improvement while reducing the risks of ARGs invasion.
The role of oxidative stress in the development of knee osteoarthritis: A comprehensive research review
Authors: Liu Lin , Luo Pan , Yang Mingyi , Wang Jiachen , Hou Weikun , Xu Peng
Published: September 2022

Knee osteoarthritis (KOA) is one of the most common degenerative diseases, and its core feature is the degeneration and damage of articular cartilage. The cartilage degeneration of KOA is due to the destruction of dynamic balance caused by the activation of chondrocytes by various factors, with oxidative stress playing an important role in the pathogenesis of KOA. The overproduction of reactive oxygen species (ROS) is a result of oxidative stress, which is caused by a redox process that goes awry in the inherent antioxidant defence system of the human body. Superoxide dismutase (SOD) inside and outside chondrocytes plays a key role in regulating ROS in cartilage. Additionally, synovitis is a key factor in the development of KOA. In an inflammatory environment, hypoxia in synovial cells leads to mitochondrial damage, which leads to an increase in ROS levels, which further aggravates synovitis. In addition, oxidative stress significantly accelerates the telomere shortening and ageing of chondrocytes, while ageing promotes the development of KOA, damages the regulation of redox of mitochondria in cartilage, and stimulates ROS production to further aggravate KOA. At present, there are many drugs to regulate the level of ROS, but these drugs still need to be developed and verified in animal models of KOA. We discuss mainly how oxidative stress plays a part in the development of KOA. Although the current research has achieved some results, more research is needed.
The hsp70 new functions as a regulator of reproduction both female and male in Ophraella communa
Authors: Zhang Yan , Ma Weihua , Ma Chao , Zhang Qinglu , Tian Zhenya , Tian Zhenqi , Chen Hongsong , Guo Jianying , Wan Fanghao , Zhou Zhongshi
Published: September 2022

Heat shock proteins (Hsps) function as molecular chaperones that enable organisms to withstand stress and maintain normal life activities. In this study, we identified heat shock protein 70 (encoded by hsp70), which exhibits a higher expression in the mature male testis than in the unmature testis of Ophraella communa. Tissue expression profile revealed that Ochsp70 levels in males were highest in the testis, whereas those in females were highest in the head. Moreover, the expression of Ochsp70 was found to be significantly induced in female bursa copulatrix after mating. Double-stranded RNA dsOchsp70 was injected into males to performance RNA interference, which significantly decreased the male Ochsp70 expression levels within 20 d post-injection, whereas no effect was observed on the Ochsp70 expression level in the females after mating with dsOchsp70-injected males. However, significant downregulation of female fertility was marked simultaneously. Furthermore, knockdown of female Ochsp70 expression also led to a significant reduction in fertility. Finally, comparative transcriptomic analysis identified glucose dehydrogenase and insulin-like growth factor binding protein as putative downstream targets of Ochsp70. Overall, we deduced that Ochsp70 is an indispensable gene and a potential male mating factor in O. communa, which regulates reproduction.
Biosynthesis of silver nanoparticles using extract of Rumex nepalensis for bactericidal effect against food-borne pathogens and antioxidant activity
Authors: Geremew Addisie , Carson Laura , Woldesenbet Selamawit
Published: September 2022

The evolution and incidence of multidrug-resistant food-borne pathogens still become a critical public health global issue. To avert this challenge there is great interest in medical applications of silver nanoparticles. Thus, this study aimed to synthesize silver nanoparticles (Rn-AgNPs) using aqueous leaf extract of Nepal Dock (Rumex nepalensis Spreng) and evaluate their antibacterial potential against food-borne pathogens and antioxidant activity. The Rn-AgNPs were characterized by UV-Vis spectrophotometry, Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM), and Fourier Transform Infra-Red Spectroscopy (FTIR). The antibacterial activities of the Rn-AgNPs were evaluated using agar well diffusion (zone of inhibition, ZOI) and microdilution (minimum inhibitory concentration, MIC and minimum bactericidal concentration, MBC) methods. The antioxidant property of the Rn-AgNPs was investigated using radical scavenging (DPPH and hydroxyl) assays. The UV-Vis spectra of Rn-AgNPs elucidated the absorption maxima at 425 nm and FTIR detected numerous functional groups of biological compounds that are responsible for capping and stabilizing Rn-AgNPs. DLS analysis displayed monodispersed Rn-AgNPs of 86.7 nm size and highly negative zeta potential (-32.5 mV). Overall results showed that Escherichia coli was the most sensitive organism, whereas Staphylococcus aureus was the least sensitive against Rn-AgNPs. In the antioxidant tests, the AgNPs radical scavenging activity reached 95.44% at 100 μg/ml. This study indicates that Rn-AgNPs exhibit a strong antimicrobial on L. monocytogenes, S. aureus, S. typhimurium, and E. coli and antioxidant and thus might be developed as a new type of antimicrobial agent for the treatment of multidrug-resistant foodborne pathogens and extensible applications in nanomaterial food- and nanocomposite-based antimicrobial packaging and/or as an antioxidant.
Probiotic supplementation and systemic inflammation in relapsing-remitting multiple sclerosis: A randomized, double-blind, placebo-controlled trial
Authors: Rahimlou Mehran , Nematollahi Shima , Husain Durdana , Banaei-Jahromi Nasrin , Majdinasab Nastaran , Hosseini Seyed Ahmad
Published: September 2022

Background

Multiple sclerosis (MS) is a complex inflammatory disease in which demyelination occurs in the central nervous system affecting approximately 2.5 million people worldwide. Intestinal microbiome changes play an important role in the etiology of chronic diseases.

Objective

This study aimed to investigate the effect of probiotic supplementation on systemic inflammation in patients with MS.

Methods

A 12-week double-blind clinical trial study was designed and seventy patients with MS were randomly divided into two groups receiving probiotics and placebo. Patients in the intervention group received two capsules containing multi-strain probiotics daily and patients in the control group received the same amount of placebo. Factors associated with systemic inflammation were assessed at the beginning and end of the study.

Results

Sixty-five patients were included in the final analysis. There was no significant difference between the two groups in terms of baseline variables except for the duration of the disease (P > 0.05). At the end of the study, probiotic supplementation compared to the placebo caused a significant reduction in the serum levels of CRP (−0.93 ± 1.62 vs. 0.05 ± 1.74, P = 0.03), TNF-α (−2.09 ± 1.88 vs. 0.48 ± 2.53, P = 0.015) and IFN-γ (−13.18 ± 7.33 vs. −1.93 ± 5.99, P < 0.001). Also, we found a significant increase in the FOXP3 and TGF-β levels in the intervention group (P < 0.05).

Conclusion

The results of our study showed that supplementation with probiotics can have beneficial effects on serum levels of some factors associated with systemic inflammation.

Clinical trial registration

[http://www.irct.ir], identifier [IRCT20181210041 918N1].
Potential mechanisms of acupuncture for neuropathic pain based on somatosensory system
Authors: Ma Xin , Chen Wen , Yang Na-Na , Wang Lu , Hao Xiao-Wan , Tan Chun-Xia , Li Hong-Ping , Liu Cun-Zhi
Published: September 2022

Neuropathic pain, caused by a lesion or disease of the somatosensory system, is common and distressing. In view of the high human and economic burden, more effective treatment strategies were urgently needed. Acupuncture has been increasingly used as an adjuvant or complementary therapy for neuropathic pain. Although the therapeutic effects of acupuncture have been demonstrated in various high-quality randomized controlled trials, there is significant heterogeneity in the underlying mechanisms. This review aimed to summarize the potential mechanisms of acupuncture on neuropathic pain based on the somatosensory system, and guided for future both foundational and clinical studies. Here, we argued that acupuncture may have the potential to inhibit neuronal activity caused by neuropathic pain, through reducing the activation of pain-related ion channels and suppressing glial cells (including microglia and astrocytes) to release inflammatory cytokines, chemokines, amongst others. Meanwhile, acupuncture as a non-pharmacologic treatment, may have potential to activate descending pain control system via increasing the level of spinal or brain 5-hydroxytryptamine (5-HT), norepinephrine (NE), and opioid peptides. And the types of endogenously opioid peptides was influenced by electroacupuncture-frequency. The cumulative evidence demonstrated that acupuncture provided an alternative or adjunctive therapy for neuropathic pain.
Altered mean apparent propagator-based microstructure and the corresponding functional connectivity of the parahippocampus and thalamus in Crohn’s disease
Authors: Qiu Yage , Li Qingshang , Wu Dongmei , Zhang Yiming , Cheng Jiahui , Cao Zhijun , Zhou Yan
Published: September 2022

Crohn’s disease (CD) is a chronic and relapsing inflammatory bowel disorder that has been shown to generate neurological impairments, which has the potential to signify disease activity in an underlying neurological manner. The objective of this study was to investigate the abnormalities of brain microstructure and the corresponding functional connectivity (FC) in patients with CD, as well as their associations with disease condition. Twenty-two patients with CD and 22 age-, gender-, and education-matched healthy controls (HCs) were enrolled in this study. All subjects underwent mean apparent propagator (MAP)-MRI and resting-state functional magnetic resonance imaging (MRI) (rs-fMRI) data collection. Each patient was evaluated clinically for the condition and duration of the disease. The MAP metrics were extracted and compared between two groups. Pearson’s correlation analysis was conducted to determine the relationship between disease characteristics and significantly abnormal MAP metrics in the CD group. Regions of interest (ROIs) for ROI-wise FC analysis were selected based on their correlation with MAP metrics. Results showed that multiple brain regions, including the parahippocampus and thalamus, exhibited statistically significant differences in MAP metrics between CD patients and HCs. Additionally, CD patients exhibited decreased FC between the left parahippocampus and bilateral thalamus, as well as the right parahippocampus and bilateral thalamus. The findings of this work provide preliminary evidence that structural abnormalities in the parahippocampal gyrus (PHG) and thalamus, as well as decreased FC between them, may reflect the degree of inflammatory of the disease and serve as brain biomarkers for evaluating CD activity.
Potential therapeutic role of SIRT1 in age- related hearing loss
Authors: Zhao Tingting , Tian Guangyong
Published: September 2022

Age-related hearing loss (ARHL) is a major public health burden worldwide that profoundly affects the daily life of elderly people. Silent information regulator 1 (SIRT1 or Sirtuin1), known as a regulator of the cell cycle, the balance of oxidation/antioxidant and mitochondrial function, has been proven to have anti-aging and life-extending effects, and its possible connection with ARHL has received increasing attention in recent years. This paper provides an overview of research on the connection between SIRT1 and ARHL. Topics cover both the functions of SIRT1 and its important role in ARHL. This review concludes with a look at possible research directions for ARHL in the future.
Structural characterization and anti-oxidation activity evaluation of pectin from Lonicera japonica Thunb.
Authors: Qi Xiaodan , Yu Yang , Wang Xinyi , Xu Jialei , Wang Xiang , Feng Zhangkai , Zhou Yifa , Xiao Hongxing , Sun Lin
Published: September 2022

Pectins are nutrient components of plants and are widely used in the food industry. In this study, one major pectin fraction (WLJP-A0.2b) with Mw of 40.6 kDa was purified from Lonicera japonica Thunb. The structural feature and antioxidant activity of it was investigated. Monosaccharide composition, Fourier transform infrared (FT-IR) spectra, enzymatic hydrolysis, and nuclear magnetic resonance (NMR) spectra analysis indicated that WLJP-A0.2b consisted of rhamnogalacturonan I (RG-I), rhamnogalacturonan II (RG-II), and homogalacturonan (HG) domains, with mass ratio of 0.4:1.0:2.1. The RG-I domain contained highly branched α-L-1,5-arabinan, β-D-1,4-galactan and type II arabinogalactan (AG-II) side chains. The HG domain was released in the form of un-esterified and partly methyl-esterified and/or acetyl-esterified oligogalacturonides with degree of polymerization 1–8 after degradation by endo-polygalacturonase. Radical scavenging assays indicated that WLJP-A0.2b exhibited antioxidant activity through the synergistic effects of different pectin domains. Oligogalacturonides, especially de-esterified oligogalacturonides, showed better antioxidant activities than RG-II and RG-I domains. Moreover, de-esterified oligogalacturonides remarkably reduced H2O2-induced reactive oxygen species production in HEK-293T cells. These results provide useful information for screening of natural antioxidants from Lonicera japonica Thunb. and application of pectin in functional food field.
Plasma arginase-1 as a predictive marker for early transarterial chemoembolization refractoriness in unresectable hepatocellular carcinoma
Authors: Xia Wei-Li , Xu Shi-Jun , Guo Yuan , Zhao Xiao-Hui , Hu Hong-Tao , Zhao Yan , Yao Quan-Jun , Zheng Lin , Zhang Dong-Yang , Guo Chen-Yang , Fan Wei-Jun , Li Hai-Liang
Published: September 2022

Objective

To explore the relationship between plasma arginase-1 (ARG1) and early transarterial chemoembolization (TACE) refractoriness in patients with hepatocellular carcinoma (HCC) and develop nomograms for predicting early TACE refractoriness.

Methods

A total of 200 patients with HCC, treated with TACE, were included in the study, including 120 in the training set and 80 in the validation set. Pre-treatment enzyme-linked immunosorbent assay was used to detected the plasma ARG1 levels of the patient, and independent predictors of early TACE refractoriness were determined using a multivariate logistic regression model, based on which a predictive model was developed using a nomogram.

Results

Risk of early TACE refractoriness was negatively correlated with plasma ARG1 levels, and multivariate logistic analysis showed tumour size (OR = 1.138, 95% CI = 1.006-1.288, P = 0.041), multiple tumors (OR=4.374, 95% CI = 1.189-16.089, P = 0.026), platelet count (OR = 0.990, 95% CI = 0.980-0.999, P = 0.036), and plasma ARG1 levels (OR = 0.209, 95% CI = 0.079-0.551, P = 0.002) to be independent prognostic factors for early TACE refractoriness.The AUC value for the nomogram of the training cohort was 0.786 (95% CI = 0.702–0.870), and the validation set AUC value was 0.833 (95% CI = 0.791-0.875).The decision curve analysis suggested that the nomogram had good clinical utility.

Conclusion

High plasma ARG1 expression was associated with a lower incidence of early TACE refractoriness. The nomogram constructed based on four independent prognostic factors could facilitate an individualised prediction of the incidence of early TACE refractoriness.
The burden of cervical cancer in China
Authors: Shen Xiu , Cheng Yiquan , Ren Fupeng , Shi Zhilong
Published: September 2022

Objective

Trends in the incidence, disability-adjusted life-years (DALYs), and mortality rate of cervical cancer remain unknown.

Methods

The average annual percent changes (AAPCs) and relative risks (RR) in the incidence, DALYs, and mortality rate were determined using a joinpoint regression analysis; the net age, period, and cohort effects on above rates were evaluated.

Results

A significant increase in age-standardized incidence (AAPC, 0.9%; 95CI: 0.8, 1.1) but significant decreases in age-standardized DALYs (AAPC, -0.4%; 95%CI: -0.60, -0.20) and the mortality rate (AAPC, -0.4%; 95CI: -0.6, –0.3) were observed. As for age-specific rates, the incidence was higher in younger age groups, and the DALYs and mortality rate were lower in older age groups. The effects of age included a slight but significant increase in the RR with advancing age from 35 to 94 years; the period effect included a significant increase in the incidence over the 2005–2019 periods; and the cohort effect included a substantial increase in the incidence from earlier to later birth cohorts.

Conclusions

The incidence of cervical cancer increased from 1990 to 2019, particularly in younger age groups, and the DALYs and mortality rate decreased in the older age groups. Furthermore, the incidence increased with age, period, and cohort.
Relationship between bronchopulmonary dysplasia phenotypes with high-resolution computed tomography score in early preterm infants
Authors: Yao Qiong , Shen Quan-li , Huang Guo-ying , Hu Xi-hong
Published: September 2022

Objective

To assess the relationship between high-resolution computed tomography (HRCT) abnormalities and clinical phenotypes of bronchopulmonary dysplasia (BPD).

Methods

A retrospective, single-center study was carried out at the Children’s Hospital of Fudan University between 2013 and 2020. Preterm infants born at ≤ 32 weeks’ gestation who were diagnosed with BPD and had HRCT between 40 and 50 weeks postmenstrual age (PMA)were included in the study. HRCT images from six pulmonary lobes were scored based on seven types of pulmonary lesions from two categories: hyperaeration lesions and parenchymal lesions. The hyperaeration score (HS) included scores of decreased attenuation, mosaic attenuation, and bulla/bleb, while the parenchymal score (PS) included those of linear lesion, consolidation, bronchial wall thickening, and bronchiectasis. All seven scores were summed up to create the total score (TS). One-way ANOVA testing or Kruskal-Wallis testing was adopted for the comparison of HRCT scores with BPD severity and clinical phenotypes. The correlation between HRCT scores and clinical phenotypes was evaluated by Spearman’s correlation analysis.

Results

A total of 81 cases were included in the study. Cases with more severe BPD had a higher TS (p = 0.01), HS (p = 0.02), PS (p = 0.02), mosaic attenuation score (p = 0.03), bulla/Bleb score (p = 0.03), and linear density score (p = 0.01). TS (r = 0.28), PS (r = 0.35), linear density (r = 0.34), and consolidation (r = 0.24) were correlated with pulmonary hypertension (PH). However, no HRCT score was significantly different between the patients with or without tracheobronchomalacia (TBM). BPD patients with a combination of lung parenchymal disease, PH, and TBM had the highest TS and HS.

Conclusion

HRCT scores correlated with BPD severity and PH in our study. HS might be a useful tool in the assessment of BPD severity while linear densities and consolidation might be helpful in predicting PH.
The median effective doses of propofol combined with two different doses of nalbuphine for adult patients during painless gastroscopy
Authors: Tang Lili , Ye Chenxuan , Wang Nan , Chen Chen , Chen Sirui , Gao Shan , Liu Xuesheng
Published: September 2022

Objective: Propofol is the most widely administered intravenous anesthetic to induce deep sedation for gastroscopy procedures. Coadministration of nalbuphine can provide analgesia and sedation to patients experiencing visceral pain, thereby decreasing the amount of propofol needed and reducing the risk of propofol-induced adverse events. We carried out this study to determine the median effective dose (ED50) of propofol in combination with different dosages of nalbuphine and the optimal dosage of nalbuphine during painless gastroscopy.

Methods: We recruited sixty-five patients aged 18–60 years who underwent elective painless gastroscopy. A total of sixty-one patients were allocated randomly to the N1 group (nalbuphine 0.1 mg/kg) or N2 group (nalbuphine 0.15 mg/kg). Three minutes after administration of nalbuphine, patients received a preset dose of propofol at 2.0 mg/kg with a dose gradient of 0.2 mg/kg according to Dixon’s “up-and-down” method. The primary outcome was the ED50 of propofol in combination with nalbuphine. Hemodynamic parameters, recovery time, pain score, and adverse events were recorded as secondary outcomes.

Results: The ED50 of propofol in the N2 group was significantly lower than that observed in the N1 group (p < 0.01). Using probit analysis, the ED50 and ED95 of propofol were 1.632 mg/kg and 2.759 mg/kg in the N1 group and 1.111 mg/kg and 2.243 mg/kg in the N2 group, respectively. The incidence of hypotension in the N2 group was lower than that in the N1 group (p < 0.05), and the recovery time was shorter than that of the N1 group (p < 0.05).

Conclusion: In adult patients, 0.15 mg/kg nalbuphine led to a significant reduction in the ED50 and ED95 of propofol during gastroscopy. This dose of nalbuphine also reduced the incidence of hypotension and shortened the recovery time. Therefore, nalbuphine (0.15 mg/kg) combined with propofol is a safe option for enhancing recovery after painless gastroscopy in adult patients.

Clinical Trial Registration: [https://www.chictr.org.cn/edit.aspx?pid=126699&htm=4], identifier [ChiCTR2100053204].
Maize stomatal responses against the climate change
Authors: Serna Laura
Published: September 2022

Drought and heat, in the context of climate change, are expected to increase in many agricultural areas across the globe. Among current abiotic stresses, they are the most limiting factors that influence crop growth and productivity. Maize is one of most widely produced crops of the world, being the first in grain production with a yield that exceeded 1.1 billion tons in 2021. Despite its wide distribution in semi-arid regions, it is highly vulnerable to climate change, which triggers important losses in its productivity. This article explores how maize yield may persevere through climate change by focusing on the stomatal regulation of gas exchange. The emerging picture unravels that maize copes with drought stress by reducing stomatal size and stomatal pore area, and increasing stomatal density, which, in turn, reduces transpiration and photosynthetic rate. When drought and heat co-occur, heat enhances stomatal response to drought stress. To avoid plant heat damage, the decline in stomatal aperture could trigger the expansion of the distance of action, from the longitudinal leaf veins, of ZmSHR1, which might act to positively regulate ZmSPCHs/ZmICE1 heterodimers, increasing the stomatal density. Only when drought is not very severe, elevated CO2 levels reduce yield losses. The knowledge of the upcoming climate changes together with the prediction of the developmental and physiological stomatal responses will allow not only to anticipate maize yield in the next years, but also to contribute to the correct decision-making in the management of this important crop.
Susceptible and resistant olive cultivars show differential physiological response to Xylella fastidiosa infections
Authors: Surano Antony , Abou Kubaa Raied , Nigro Franco , Altamura Giuseppe , Losciale Pasquale , Saponari Maria , Saldarelli Pasquale
Published: September 2022

Olive quick decline syndrome (OQDS) is a severe disease, first described in Italy in late 2013, caused by strains ofXylella fastidiosasubsp.pauca (Xfp)in susceptible olive cultivars. Conversely, resistant olive cultivars do not develop OQDS but present scattered branch dieback, which generally does not evolve to severe canopy decline. In the present study, we assessed the physiological responses ofXfp-infected olive trees of susceptible and resistant cultivars. Periodic measurements of stomatal conductance (gs) and stem water potential (Ψstem) were performed using a set of healthy andXfp-infected plants of the susceptible “Cellina di Nardò” and resistant “Leccino” and “FS17” cultivars. Strong differences in Δgsand ΔΨstem amongXfp-infected trees of these cultivars were found, with higher values in Cellina di Nardò than in Leccino and FS17, while no differences were found among healthy plants of the different cultivars. Both resistant olive cultivars showed lower water stress uponXfpinfections, compared to the susceptible one, suggesting that measurements of gsand Ψstem may represent discriminating parameters to be exploited in screening programs of olive genotypes for resistance toX. fastidiosa.
Integrated metabolomics and transcriptomics insights on flavonoid biosynthesis of a medicinal functional forage, Agriophyllum squarrosum (L.), based on a common garden trial covering six ecotypes
Authors: Fang Tingzhou , Zhou Shanshan , Qian Chaoju , Yan Xia , Yin Xiaoyue , Fan Xingke , Zhao Pengshu , Liao Yuqiu , Shi Liang , Chang Yuxiao , Ma Xiao-Fei
Published: September 2022

Agriophyllum squarrosum (L.) Moq., well known as sandrice, is an important wild forage in sandy areas and a promising edible and medicinal resource plant with great domestication potential. Previous studies showed flavonoids are one of the most abundant medicinal ingredients in sandrice, whereby isorhamnetin and isorhamnetin-3-glycoside were the top two flavonols with multiple health benefits. However, the molecular regulatory mechanisms of flavonoids in sandrice remain largely unclear. Based on a common garden trial, in this study, an integrated transcriptomic and flavonoids-targeted metabolomic analysis was performed on the vegetative and reproductive periods of six sandrice ecotypes, whose original habitats covered a variety of environmental factor gradients. Multiple linear stepwise regression analysis unveiled that flavonoid accumulation in sandrice was positively correlated with temperature and UVB and negatively affected by precipitation and sunshine duration, respectively. Weighted co-expression network analysis (WGCNA) indicated the bHLH and MYB transcription factor (TF) families might play key roles in sandrice flavonoid biosynthesis regulation. A total of 22,778 differentially expressed genes (DEGs) were identified between ecotype DL and ecotype AEX, the two extremes in most environmental factors, whereby 85 DEGs could be related to known flavonoid biosynthesis pathway. A sandrice flavonoid biosynthesis network embracing the detected 23 flavonoids in this research was constructed. Gene families Plant flavonoid O-methyltransferase (AsPFOMT) and UDP-glucuronosyltransferase (AsUGT78D2) were identified and characterized on the transcriptional level and believed to be synthases of isorhamnetin and isorhamnetin-3-glycoside in sandrice, respectively. A trade-off between biosynthesis of rutin and isorhamnetin was found in the DL ecotype, which might be due to the metabolic flux redirection when facing environmental changes. This research provides valuable information for understanding flavonoid biosynthesis in sandrice at the molecular level and laid the foundation for precise development and utilization of this functional resource forage.
Annexation in right-wing Israeli discourse—The case of Ribonut
Authors: Lustick Ian S.
Published: September 2022

The one-state reality has dramatically changed the discursive landscape of the movement of Israeli settlers and their supporters who have waged a half century campaign to prevent territorial compromise as a basis for Israeli-Palestinian peace. Analysis of the discourse of theSovereigntymovement, as reflected in the pages of its journal,Ribonut, traces these changes and highlights the new challenges posed to the annexationist project by the prospect of the attainability of its goals and the necessity to confront the “Arab problem.”
Extreme Weather Events and Spiraling Debt: A Double Whammy for Bangladeshis Affected by Climate Change
Authors: Atiqul Haq Shah Md
Published: September 2022

This study explores how people living in different areas of Bangladesh prone to extreme weather events (EWEs) in the form of floods, cyclones, or droughts perceive climate change, the impacts they suffer in the face of EWEs, and how they cope with their consequences. Qualitative data was collected through in-depth interviews with 73 respondents from three different areas of Bangladesh and subsequently analyzed. The results show that there are similarities and differences between respondents from regions with different vulnerabilities in terms of their views and perceptions about what climate change is its causes, the consequences of EWEs, and the strategies they adopt to cope with their effects. Respondents understood climate change based on their own local experiences of climate change and EWEs. A main finding is that people in all three areas are driven to borrow money in the face of these events as a survival strategy and to be able to continue to support their families. As the climate is set to change rapidly and EWEs to occur more frequently and regularly, it will become routine for those most vulnerable to them to have to cope and live with their impacts. Increased reliance on borrowing risks leading to a debt spiral for already vulnerable people. They are thus subject to a “double whammy”: on the one hand the direct effects of climate change and EWEs on their lives and livelihoods and on the other getting caught in a debt spiral sparked by times of crisis.
Analysis on the linguistic features of conflict discourse in mathematical cooperation problem solving in China
Authors: Zhao Jingbo , Song Tingting , Song Xiaoying , Bai Yuanmeng
Published: September 2022

Classroom teaching is a kind of social activity system. Thus, as a form of classroom learning, collaborative problem solving has a strong social attribute. It is extremely common to choose the conflict discourse in the context of cooperation. The verbal characteristics of the conflicting discourse level in cooperative mathematics problem solving directly affects the cooperative learning between students and the classroom teaching of teachers. This article focuses on the overall linguistic characteristics of conflict discourse in solving cooperative problems and the discourse style and language characteristics of the three stages of conflict discourse. The main research conclusions are as follows: (1) The classification of language features of conflict discourse includes extreme summaries, negation, discourse markers, and so on. Among them, the frequency of Indexical 2nd-person pronouns is the highest. (2) The language expressions at the “initial stage of conflict” include Explanatory statement Negative response, instruct refuse and Seditious inquiry Confrontational answer. The language shows the characteristics of using emphatic words or phrases, negative words, imperative sentences and so on. Meanwhile, rebuttal questions, direct responses, explanations, and negative avoidance are the main forms language expressions at the “conflict stage.” It also exhibits the verbal characteristics of rhetorical questions, negative comments, and direct negation. Lastly, topic-shifting, compromise, third-party intervention, and one-sided wins are the linguistic expressions at the “end of conflict.” The language features are the appearance of tone relaxation and language easing, and the conflict ending utterances reflect cooperation.
The effect of director-deputy director promotion focus congruence on team knowledge creation: A social identification perspective
Authors: Yan Xue , Liu Jiakun
Published: September 2022

As a new leadership style, promotion-focused leadership has attracted the attention of theorists and practitioners. Existing research emphasizes the positive value of director personal promotion focus on team creative behavior while overlooking director-deputy director promotion focus fit. Based on Regulatory Fit Theory and Social Identity Theory, this study explored the effect of director-deputy director promotion focus fit on team knowledge creation and the mediating role of team identification. We used polynomial regression and response surface analysis to analyze the data from 674 questionnaires. The results demonstrate that: (1) director-deputy director congruence in promotion focus is positively related to team identification; (2) under the condition of director-deputy director promotion focus congruence, the level of team identification does not significantly increase when director-deputy director promotion focus rises from “low-low” to “high-high”; (3) team identification plays a mediating role in the relationship between director-deputy director promotion focus congruence and team knowledge creation.
Examining the relationship between perceived teacher support and students’ academic engagement in foreign language learning: Enjoyment and boredom as mediators
Authors: Zhao Yanlin , Yang Lianrui
Published: September 2022

As an important contextual factor influencing various aspects of students’ learning, teacher support has been widely explored in general education but largely overlooked in the English as a Foreign Language (EFL) context. Given its potential positive role in students’ academic performance, the present study intended to investigate the relationship between perceived teacher support, enjoyment, boredom, and academic engagement in the EFL context. In so doing, 1094 Chinese high school students were recruited to complete the online questionnaire of the four variables. SPSS and PROCESS macro were used for descriptive, correlational, and mediation analyses. The results showed that students had moderate levels of perceived teacher support, enjoyment, and academic engagement and a low level of boredom. Further correlation analyses indicated medium to large correlations between perceived teacher support, enjoyment, boredom, and academic engagement. Enjoyment and boredom collectively mediated the relationship between perceived teacher support and academic engagement. Directions for future research and implications for researchers and educators are presented at the end.
Analysis on the literature communication path of new media integrating public mental health
Authors: Liu Shaojing
Published: September 2022

The communication of mental health is an important branch of health communication, and it is also an important factor affecting people’s physical and mental health. With the increasing pressure of life, people’s mental health problems have huge challenges. Under the enormous pressure of economy and life, people’s mental health problems are becoming increasingly prominent. This calls for attention to mental health issues. In the context of new media, knowledge about mental health can be disseminated through the Internet and mobile platforms. This approach will spread awareness of mental health prevention and basic issues. Mental health problems are also a manifestation of the lack of humanistic spirit. Excellent works related to humanistic spirit can promote the relief of mental health problems. Literature can contribute to the development of mental health problems. This research studies the communication of mental health issues in the context of new media using literary works as a carrier. At the same time, it also considers big data-related algorithms to mine the traditional characteristics of mental health problems. The research results show that new media technology can well assist the dissemination of mental health education, and literary works also contribute to the dissemination of mental health education knowledge. Collaborative filtering algorithm and atrous convolution algorithm can better predict the relevant characteristics in the process of mental health communication. For the CF algorithm, its maximum similarity index reached 0.987 when recommending mental health propagation paths using new media technology. For ACNN, the smallest prediction error is only 1.78%.
Case report: Green nail syndrome in an epidemic prevention volunteer during the outbreak of the Omicron in Shanghai
Authors: Yu Qian , Hu Sheng , Li Wei , Yang Lianjuan
Published: September 2022

Green nail syndrome (GNS) is an infectious disorder characterized by greenish discoloration of the nail plate. Pseudomonas aeruginosa is the most common organism that causes GNS. It is an opportunistic human pathogen that preferentially colonizes moist environments, and thus, it usually affects patients with a history of prolonged exposure to moist environments. Here, we describe a case of GNS in an epidemic prevention volunteer that was caused by wearing personal protective equipment for prolonged durations. The case was reported during the outbreak caused by the SARS-CoV-2 Omicron variant in Shanghai. After receiving information about his condition and proper treatment, the patient was cured.
The influence of professional identity, job satisfaction, burnout on turnover intention among village public health service providers in China in the context of COVID-19: A cross-sectional study
Authors: Zhang Xuewen , Zhang Wenjie , Xue Li , Xu Zongyou , Tian Zhuang , Wei Chao , Zhang Ying , Dong Zhihuan , Gao Shansong
Published: September 2022

Background

In China, COVID-19 has undoubtedly posed a huge challenge to the capacity of rural public health services. Village public health service providers are responsible for reporting and dealing with infectious diseases and public health emergencies. However, the turnover of village public health service providers is gravely threatening the stability of rural primary health system step by step. This study systematically evaluated the effects of professional identity, job satisfaction, burnout on turnover intention of village public health service providers, and further measured the mediating effect of job satisfaction and burnout between professional identity and turnover intention.

Methods

From May to June 2019, 1,244 village public health service providers in Shandong Province were selected as the research objects. Sociodemographic characteristics, professional identity, job satisfaction, burnout and turnover intention were quantitated by self-completed questionnaire and measured by Likert 5–7 scale. Person correlation analysis, One-way ANOVA, and Structural Equation Modeling (SEM) were used for statistical analysis and mediating effect evaluation.

Results

Five hundred and sixty-four (45.3%) village public health providers had high turnover intension. Professional identity had a direct positive effect on job satisfaction (β = 0.146, p < 0.001), job satisfaction had a direct negative effect on burnout (β = 0.263, p < 0.001), and turnover intension (β = −0.453, p < 0.001), while burnout had a direct positive effect on turnover intension (β = 0.242, p < 0.001). Between professional identity and turnover intention, job satisfaction 95%CI: (−0.289)–(−0.11) had significant mediating effects. Job satisfaction 95%CI: (−0.216)–(−0.077) also had significant mediating effects between professional identity and burnout, and burnout had significant mediating effects between job satisfaction and turnover intension, 95%CI: (−0.116)–(−0.052). These results strongly confirm that professional identity, job satisfaction, and burnout are early and powerful predicators of turnover intention.

Conclusion

According to the results, medical administration and management departments should pay attention to improve the professional attraction of public health services by improving the public's understanding of the profession, reducing work intensity, timely granting of subsidy funds, improving old-age security, ensuring the income level, increasing the new force and so on, so as to reduce turnover intension and ensure the health equity of village residents.
Factors influencing smoking cessation counselors' intention to stay: An application of a conceptual model of intention to stay verified with path analysis
Authors: Liu Yi-Chun , Chiang Shih-Hung , Lai Chung-Yu , Yen Li-Chen , Liaw Fang-Yih , Lin Ming-Han , Lin Fu-Gong , Lai Ching-Huang , Kao Senyeong , Chang Yu-Tien , Wu Chia-Chao , Chiu Yu-Lung
Published: September 2022

Background

The Taiwanese military trains smoking cessation counselors to counsel officers and soldiers on quitting smoking as part time. The intention to stay among smoking cessation counselors affects the promotion of smoking cessation. This study investigated smoking cessation counselors' intention to stay by applying a conceptual model of intent to stay (CMIS) to analyze influencing factors.

Methods

In this cross-sectional study, we applied the CMIS to design a questionnaire. We invited 577 smoking cessation counselors trained in the military from 2016 to 2017. The response rate was 46.7%, and the questionnaire responses of 260 military smoking cessation counselors were analyzed. We used path analysis to verify the relationships among the various aspects of the CMIS.

Results

We determined that smoking cessation counselors' intention to stay is directly affected by job satisfaction (β = 0.150, p = 0.014), job stress (β = −0.225, p < 0.001), and institutional identification (β = 0.431, p < 0.001). Career opportunities indirectly affect intention to stay through institutional identification, working environment indirectly affects intention to stay through job stress, and co-worker support and self-fulfillment indirectly affect intention to stay through job satisfaction and institutional identification. Our model could explain 36.7% of the variance in intent to stay among smoking cessation counselors.

Conclusion

Our results suggest that relevant policies should be formulated to enhance smoking cessation counselors' recognition, affirmation, and sense of belonging as related to smoking cessation counseling work, thereby raising their institutional identification and promoting their intention to stay.
Parallel randomized controlled feasibility trials of the “Active Brains” digital intervention to protect cognitive health in adults aged 60–85
Authors: Essery Rosie , Pollet Sebastien , Bradbury Katherine , Western Max J. , Grey Elisabeth , Denison-Day James , Smith Kirsten A. , Hayter Victoria , Kelly Joanne , Somerville Jane , Stuart Beth , Becque Taeko , Zhang Jin , Slodkowska-Barabasz Joanna , Mowbray Fiona , Ferrey Anne , Yao Guiqing , Zhu Shihua , Kendrick Tony , Griffin Simon , Mutrie Nanette , Robinson Sian , Brooker Helen , Griffiths Gareth , Robinson Louise , Rossor Martin , Ballard Clive , Gallacher John , Rathod Shanaya , Gudgin Bernard , Phillips Rosemary , Stokes Tom , Niven John , Little Paul , Yardley Lucy
Published: September 2022

Introduction

Multidomain interventions to address modifiable risk factors for dementia are promising, but require more cost-effective, scalable delivery. This study investigated the feasibility of the “Active Brains” digital behavior change intervention and its trial procedures.

Materials and methods

Active Brains aims to reduce cognitive decline by promoting physical activity, healthy eating, and online cognitive training. We conducted 12-month parallel-design randomized controlled feasibility trials of “Active Brains” amongst “lower cognitive scoring” (n = 180) and “higher cognitive scoring” (n = 180) adults aged 60–85.

Results

We collected 67.2 and 76.1% of our 12-month primary outcome (Baddeley verbal reasoning task) data for the “lower cognitive score” and “higher cognitive score” groups, respectively. Usage of “Active Brains” indicated overall feasibility and satisfactory engagement with the physical activity intervention content (which did not require sustained online engagement), but engagement with online cognitive training was limited. Uptake of the additional brief telephone support appeared to be higher in the “lower cognitive score” trial. Preliminary descriptive trends in the primary outcome data might indicate a protective effect of Active Brains against cognitive decline, but further investigation in fully-powered trials is required to answer this definitively.

Discussion

Whilst initial uptake and engagement with the online intervention was modest, it was in line with typical usage of other digital behavior change interventions, and early indications from the descriptive analysis of the primary outcome and behavioral data suggest that further exploration of the potential protective benefits of Active Brains are warranted. The study also identified minor modifications to procedures, particularly to improve online primary-outcome completion. Further investigation of Active Brains will now seek to determine its efficacy in protecting cognitive performance amongst adults aged 60–85 with varied levels of existing cognitive performance.