bluuebunny/crossref_metadata_2025 huggingface dataset:
Primary versus Salvage Liver Transplantation after Curative-Intent Resection or Radiofrequency Ablation for Hepatocellular Carcinoma: Long-Term Oncological Outcomes
Authors: Anselmo Alessandro , Siragusa Leandro , Brigato Paolo , Riccetti Camilla , Collini Andrea , Sensi Bruno , Tisone Giuseppe
Published: October 2023

Liver transplantation for hepatocellular carcinoma (HCC) may be performed ab initio, primary liver transplantation (PLT), or for HCC recurrence after previous treatments such as liver resection (LR) or radiofrequency ablation (RFA), salvage liver transplantation (SLT). The aim of this study was to evaluate the oncological outcomes of SLT vs. PLT. For this, a retrospective study was carried out on patients undergoing liver transplantation for HCC. The outcomes of PLT were compared with those of SLT. The primary outcome was disease-free survival (DFS). The secondary outcomes included overall survival (OS), cancer-specific survival (CSS), and major postoperative complications. A sub-analysis of SLT-LR and SLT-RFA was also performed. In total, 141 patients were included: 96 underwent PLT and 45 SLT. Among the SLT group, 25 patients had undergone previous LR while 20 had had RFA. There were no differences in the major postoperative complications. Unadjusted DFS was significantly longer in the PLT group (p = 0.02), as were OS (p = 0.025) and CSS (p = 0.001). There was no difference in DFS between PLT and SLT-LR groups, while a significant difference was found between the PLT and SLT-RFA groups (p = 0.035). Nonetheless, DFS was no different between the SLT-LR and SLT-RFA groups. PLT appears to offer superior long-term oncological outcomes to SLT. Both SLT-LR and SLT-RFA offer acceptable OS and CSS. Further prospective studies are needed to confirm these results, but the re-direction of grafts and transplant philosophy towards PLT rather than SLT may need to be considered.
YAP1 Expression in HR+HER2− Breast Cancer: 21-Gene Recurrence Score Analysis and Public Dataset Validation
Authors: Park Inho , Lee Yangkyu , Kim Jee Hung , Bae Soong June , Ahn Sung Gwe , Jeong Joon , Cha Yoon Jin
Published: October 2023

Background: YAP1, an oncogene in numerous cancers, is a downstream transcription factor of the Hippo pathway. This study focuses on its relationship with the Oncotype Dx (ODX) test risk score (RS) in patients with hormone-receptor-positive, HER2-negative (HR+HER2−) breast cancer. Methods: We retrospectively analyzed 401 HR+HER2− breast cancer patients from Gangnam Severance Hospital who underwent ODX tests (May 2014–April 2020). YAP1 nuclear localization was evaluated via immunohistochemical staining and its clinical correlation with clinicopathological parameters, including RS, was analyzed. Public datasets TCGA-BRCA and METABRIC validated clinical outcomes. Results: YAP1 expression negatively correlated with ODX RS (OR 0.373, p = 0.002). Elevated YAP1 mRNA levels corresponded to better clinical outcomes, specifically in ER-positive patients, with significant results in METABRIC and TCGA-BRCA datasets (p < 0.0001 OS in METABRIC, p = 0.00085 RFS in METABRIC, p = 0.040 DFS in TCGA-BRCA). In subsets with varying ESR1 mRNA expression and pronounced YAP1 expression, superior survival outcomes were consistently observed. Conclusion: YAP1 may be a valuable prognostic marker and potential therapeutic target in HR+HER2− breast cancer patients.
Consumer Perspectives on the Adoption of a Prehabilitation Multimodal Online Program for Patients Undergoing Cancer Surgery
Authors: Steffens Daniel , Denehy Linda , Solomon Michael , Koh Cherry , Ansari Nabila , McBride Kate , Carey Sharon , Bartyn Jenna , Lawrence Aaron Sean , Sheehan Kym , Delbaere Kim
Published: October 2023

This study aimed to explore patients’ perspectives on the adoption of a prehabilitation multimodal online program. Patients recovering from gastrointestinal cancer surgery at a tertiary hospital between October 2021 and November 2022 were invited to participate. An e-Health program including intensity exercises, nutrition and psychological counselling was used. Patients were instructed to navigate the e-Health program over 24 h using an iPad and then complete the study survey. Patients’ characteristics, use of technology, views and minimal expected outcomes from a preoperative online program were collected. Of the 30 patients included, most were female, most reported confidence in the use of technology, most considered the online program safe and most agreed it would be beneficial for their health. “Poor preoperative health” and “lack of motivation and encouragement” were identified as the main barriers to the uptake of a preoperative online program, while program ‘simplicity’ and perceived ‘benefits’ were the main facilitators. Significant improvement in postoperative outcomes is perceived to influence patients’ willingness to participate in a preoperative multimodal e-Health program. Gastrointestinal cancer patients perceived the adoption of a preoperative multimodal e-Health application as safe to be performed at home and of potential benefit to their health. A range of patient’s characteristics, barriers and facilitators to the uptake of an online program were identified. These should be considered in future preoperative multimodal online programs to enhance patient experience, adherence and efficacy. The safety and efficacy of the online prehabilitation program will need to be determined in a larger randomized controlled trial.
Synthesis and Characterization of Acid-Activated Carbon Prepared from Sugarcane Bagasse for Furfural Production in Aqueous Media
Authors: Silva Thiago Alves Lopes , da Silva Adilson Candido , Pasquini Daniel
Published: October 2023

Furfural is a platform molecule obtained from hemicellulosic monosaccharides present in lignocellulosic biomass. Due to the possibility of converting this molecule into several value-added chemicals and the need to search for more sustainable production processes, the present work aimed to produce and characterize different sulfonated activated carbons (AC-S) from sugarcane bagasse (SCB) for application in furfural production in aqueous media. ACs were produced by chemical activation using salts of ZnCl2, NiCl2, and CuCl2 and a temperature and activation time of 550 °C and 3 h under nitrogen flow, respectively. Sulfonation was carried out with H2SO4 (98%) at a solid/liquid ratio of 1:10 at 160 °C for 2 h. Catalytic tests were performed using 5% catalyst mass regarding xylose, a temperature of 180 °C, and a reaction time of 2 h. ACs with high surface areas, ranging from 290 to 1100 m2 g−1, were produced. All catalysts had an increased sulfur content and total acidity after sulfonation, indicating the successful attachment of the sulfonic group (-SO3H) in the carbon matrix of the CAs. The AC-S/CuCl2 catalyst achieved the best catalytic performance compared to AC-S/ZnCl2, AC-S/NiCl2, and other acidic solids reported in the literature, achieving yield and selectivity of 55.96% and 83.93%, respectively. These results evidence the importance of the synergy between the Lewis and Brønsted acid sites on selective xylose dehydration and make AC-S/CuCl2 a promising acid catalyst for converting xylose to furfural in an aqueous medium.
The Synthesis of Ginsenoside Compound K Using a Surface-Displayed β-Glycosidase Whole-Cell Catalyst
Authors: Guo Lianxia , Li Tao , Guo Gege , Liu Zhaoxing , Hao Ning
Published: October 2023

Ginsenoside compound K (CK) has garnered considerable attention due to its versatile pharmacological properties, including anti-inflammatory, anti-allergic, anti-aging, anti-diabetic, and hepatoprotective effects, along with neuroprotection. The conventional approach to synthesizing ginsenoside CK involves enzymatic conversion. However, the purification of enzymes necessitates effort and expense, and enzymes are prone to inactivation. Additionally, whole-cell catalysis suffers from inefficiency due to limited cell permeability. To address these challenges, we harnessed the YiaT protein as an anchoring motif, establishing a surface display system for β-glycosidase Bgp3. This innovative system served as a whole-cell catalyst for the efficient synthesis of ginsenoside CK. We further optimized the YiaT-Bgp3 system, enhancing display levels and significantly increasing ginsenoside CK production. Optimal conditions were achieved at an IPTG concentration of 0.5 mM, an induction temperature of 16 °C, a ginsenoside substrate concentration of 15 mg/mL, and a catalytic temperature of 30 °C. Ultimately, the YiaT-Bgp3 system synthesized 5.18 ± 0.08 mg/mL ginsenoside CK within 24 h, with a conversion of 81.83 ± 1.34%. Furthermore, the YiaT-Bgp3 system exhibited good reusability, adding to its practicality and value. This study has successfully developed an efficient whole-cell Bgp3 biocatalyst, offering a convenient, highly productive, and economically viable solution for the industrial production of ginsenoside CK.
Interaction of the C9orf72-Amyotrophic Lateral Sclerosis-Related Proline–Arginine Dipeptide Repeat Protein with the RNA-Binding Protein NOVA1 Causes Decreased Expression of UNC13A Due to Enhanced Inclusion of Cryptic Exons, Which Is Reversed by Betulin Treatment
Authors: Fu Ru-Huei , Chen Hui-Jye , Hong Syuan-Yu
Published: October 2023

C9orf72 mutations are the most common form of familial amyotrophic lateral sclerosis (C9-ALS). It causes the production of proline–arginine dipeptide repeat proteins (PR-DPRs) in motor neurons (MNs), leading to the molecular pathology characteristic of ALS. UNC13A is critical for maintaining the synaptic function of MNs. Most ALS patients have nuclear deletion of the splicing repressor TDP-43 in MNs, which causes inclusion of the cryptic exon (CE) of UNC13A mRNA, resulting in nonsense-mediated mRNA decay and reduced protein expression. Therefore, in this study, we explored the role of PR-DPR in CE inclusion of UNC13A mRNA. Our results showed that PR-DPR (PR50) induced CE inclusion and decreased the protein expression of UNC13A in human neuronal cell lines. We also identified an interaction between the RNA-binding protein NOVA1 and PR50 by yeast two-hybrid screening. NOVA1 expression is known to be reduced in patients with ALS. We found that knockdown of NOVA1 enhanced CE inclusion of UNC13A mRNA. Furthermore, the naturally occurring triterpene betulin can inhibit the interaction between NOVA1 and PR50, thus preventing CE inclusion of UNC13A mRNA and protein reduction in human neuronal cell lines. This study linked PR-DPR with CE inclusion of UNC13A mRNA and developed candidate therapeutic strategies for C9-ALS using betulin.
Effect of Hyperbaric Oxygen and Inflammation on Human Gingival Mesenchymal Stem/Progenitor Cells
Authors: Tölle Johannes , Koch Andreas , Schlicht Kristina , Finger Dirk , Kaehler Wataru , Höppner Marc , Graetz Christian , Dörfer Christof , Schulte Dominik M. , Fawzy El-Sayed Karim
Published: October 2023

The present study explores for the first time the effect of hyperbaric oxygen (HBO) on gingival mesenchymal stem cells’ (G-MSCs) gene expression profile, intracellular pathway activation, pluripotency, and differentiation potential under an experimental inflammatory setup. G-MSCs were isolated from five healthy individuals (n = 5) and characterized. Single (24 h) or double (72 h) HBO stimulation (100% O2, 3 bar, 90 min) was performed under experimental inflammatory [IL-1β (1 ng/mL)/TNF-α (10 ng/mL)/IFN-γ (100 ng/mL)] and non-inflammatory micro-environment. Next Generation Sequencing and KEGG pathway enrichment analysis, G-MSCs’ pluripotency gene expression, Wnt-/β-catenin pathway activation, proliferation, colony formation, and differentiation were investigated. G-MSCs demonstrated all mesenchymal stem/progenitor cells’ characteristics. The beneficial effect of a single HBO stimulation was evident, with anti-inflammatory effects and induction of differentiation (TLL1, ID3, BHLHE40), proliferation/cell survival (BMF, ID3, TXNIP, PDK4, ABL2), migration (ABL2) and osteogenic differentiation (p < 0.05). A second HBO stimulation at 72 h had a detrimental effect, significantly increasing the inflammation-induced cellular stress and ROS accumulation through HMOX1, BHLHE40, and ARL4C amplification and pathway enrichment (p < 0.05). Results outline a positive short-term single HBO anti-inflammatory, regenerative, and differentiation stimulatory effect on G-MSCs. A second (72 h) stimulation is detrimental to the same properties. The current results could open new perspectives in the clinical application of short-termed HBO induction in G-MSCs-mediated periodontal reparative/regenerative mechanisms.
The Lymphatic Endothelial Cell Secretome Inhibits Osteoblast Differentiation and Bone Formation
Authors: Solorzano Ernesto , Alejo Andrew L. , Ball Hope C. , Robinson Gabrielle T. , Solorzano Andrea L. , Safadi Rama , Douglas Jacob , Kelly Michael , Safadi Fayez F.
Published: October 2023

Complex lymphatic anomalies (CLAs) are a set of rare diseases with unique osteopathic profiles. Recent efforts have identified how lymphatic-specific somatic activating mutations can induce abnormal lymphatic formations that are capable of invading bone and inducing bone resorption. The abnormal bone resorption in CLA patients has been linked to overactive osteoclasts in areas with lymphatic invasions. Despite these findings, the mechanism associated with progressive bone loss in CLAs remains to be elucidated. In order to determine the role of osteoblasts in CLAs, we sought to assess osteoblast differentiation and bone formation when exposed to the lymphatic endothelial cell secretome. When treated with lymphatic endothelial cell conditioned medium (L-CM), osteoblasts exhibited a significant decrease in proliferation, differentiation, and function. Additionally, L-CM treatment also inhibited bone formation through a neonatal calvaria explant culture. These findings are the first to reveal how osteoblasts may be actively suppressed during bone lymphatic invasion in CLAs.
Niobium Oxide Nanorods Obtained by Hydrothermal Synthesis—Structure, Morphology, and Electrochemical Detection of Oxygen Via Oxygen Reduction Reaction
Authors: Vega Maria Rita Ortega , Consul Viviane , Cadorin Martina , Arenas Leliz Ticona , Aguzzoli César , Hübler Roberto , Takimi Antônio , de Fraga Malfatti Célia
Published: October 2023

Niobium oxides are promising materials for applications within various research fields, especially as electrocatalysts for various chemical reactions. The tuning of the synthetic parameters can achieve a successful compromise between morphology and structure, aiming to obtain certain properties. Hence, this study aimed to investigate the influence of hydrothermal synthesis parameters on the morphology and structure of niobium oxide growth on a niobium metallic plate. The effect of annealing on the material performance was also evaluated. Afterward, the most crystalline sample was tested for the electrochemical determination of dissolved oxygen, a fundamental reaction in corrosion, biomedicine, and environmental monitoring. This is the first work using this material configuration as an electrochemical sensor. The hydrothermal synthesis produced nanorods formed by poorly crystalline, acidic, hydrated Nb2O5. Increasing the mineralizer concentration could increase the crystallinity and the nanorod growth rate, but it could also promote a lack of structural and morphological uniformity throughout the surface. Heat treatment allowed the increase in crystallinity and favored orthorhombic Nb2O5. Raman spectroscopy revealed that, at the first moment, acidic, hydrated niobium oxide structures were formed as precursors of crystalline niobium oxide that would be developed with longer reaction times and a higher mineralizer concentration. The obtained niobium oxide showed electrocatalytic activity toward the oxygen reduction reaction, with comparable performance between the samples with and without heat treatment. At all analyzed pH values, the amperometric response was linearly correlated with the dissolved oxygen concentration. pH influenced the sensitivity of the material; a maximum sensitivity of 0.0417 mA/cm2·mg/L O2 was achieved at pH = 6. The participation of the acidic functionalities of the surface in the ORR reaction was confirmed by Raman spectroscopy.
Spin-Polarized 2D Electron Gas at the EuTiO3/EuO Interface
Authors: Gjerde Jonathan , Hammouri Mahmoud , Jishi Radi A.
Published: October 2023

Perovskite oxide heterostructures have provided opportunities for new technologies and materials with novel properties.In particular, researchers have been interested in the magnetism and two-dimensional electron gases that form at the interface of many of these heterostructures. Often, these properties are due to polar discontinuities, but here we use first-principles calculations to examine a heterostructure whose elements, EuO and EuTiO3, are both non-polar, yet are still conductive due to oxygen vacancies. Furthermore, the free electrons at the interface of this heterostructure are predicted to be spin-polarized, opening up possibilities for future research and devices.
A Case of Paraneoplastic Anti-TIF1-γ Antibody-Positive Dermatomyositis Presenting with Generalized Edema and Associated with Aortic Aneurysm
Authors: Bennett Raven , Bradley Katherine , Salem Iman , Weiner David , Patel Dhrumil , Cloutier Jeffrey , Pace Nicole , Barton Dorothea
Published: October 2023

Dermatomyositis (DM) is a rare autoimmune inflammatory disease characterized by pathognomonic skin findings, often accompanied by myositis beginning with proximal weakness [...]
Diagnostic Role and Prognostic Impact of PSAP Immunohistochemistry: A Tissue Microarray Study on 31,358 Cancer Tissues
Authors: Tribian Laura Sophie , Lennartz Maximilian , Höflmayer Doris , de Wispelaere Noémi , Dwertmann Rico Sebastian , von Bargen Clara , Kind Simon , Reiswich Viktor , Viehweger Florian , Lutz Florian , Bertram Veit , Fraune Christoph , Gorbokon Natalia , Weidemann Sören , Hube-Magg Claudia , Menz Anne , Uhlig Ria , Krech Till , Hinsch Andrea , Burandt Eike , Sauter Guido , Simon Ronald , Kluth Martina , Steurer Stefan , Marx Andreas H. , Lebok Patrick , Dum David , Minner Sarah , Jacobsen Frank , Clauditz Till S. , Bernreuther Christian
Published: October 2023

Prostate-specific acid phosphatase (PSAP) is a marker for prostate cancer. To assess the specificity and prognostic impact of PSAP, 14,137 samples from 127 different tumor (sub)types, 17,747 prostate cancers, and 76 different normal tissue types were analyzed via immunohistochemistry in a tissue microarray format. In normal tissues, PSAP staining was limited to the prostate epithelial cells. In prostate cancers, PSAP was seen in 100% of Gleason 3 + 3, 95.5% of Gleason 4 + 4, 93.8% of recurrent cancer under androgen deprivation therapy, 91.0% of Gleason 5 + 5, and 31.2% of small cell neuroendocrine cancer. In non-prostatic tumors, PSAP immunostaining was only found in 3.2% of pancreatic neuroendocrine tumors and in 0.8% of diffuse-type gastric adenocarcinomas. In prostate cancer, reduced PSAP staining was strongly linked to an advanced pT stage, a high classical and quantitative Gleason score, lymph node metastasis, high pre-operative PSA levels, early PSA recurrence (p < 0.0001 each), high androgen receptor expression, and TMPRSS2:ERG fusions. A low level of PSAP expression was linked to PSA recurrence independent of pre- and postoperative prognostic markers in ERG-negative cancers. Positive PSAP immunostaining is highly specific for prostate cancer. Reduced PSAP expression is associated with aggressive prostate cancers. These findings make PSAP a candidate marker for prognostic multiparameter panels in ERG-negative prostate cancers.
A Non-Stationary Cluster-Based Channel Model for Low-Altitude Unmanned-Aerial-Vehicle-to-Vehicle Communications
Authors: Su Zixv , Li Changzhen , Chen Wei
Published: October 2023

Under the framework of sixth-generation (6G) wireless communications, the unmanned aerial vehicle (UAV) plays an irreplaceable role in a number of communication systems. In this paper, a novel cluster-based low-altitude UAV-to-vehicle (U2V) non-stationary channel model with uniform planar antenna arrays (UPAs) is proposed. In order to comprehensively model the scattering environment, both single and twin clusters are taken into account. A novel continuous cluster evolution algorithm that integrates time evolution and array evolution is developed to capture channel non-stationarity. In the proposed algorithm, the link between the time evolution of twin clusters and that of single clusters is established to regulate the temporal evolution trend. Moreover, an improved observable radius method is applied to UPAs for the first time to describe array evolution. Based on the combination of cluster evolution and time-variant channel parameters, some vital statistical properties are derived and analyzed, including space–time correlation function (ST-CF), angular power spectrum density (PSD), Doppler PSD, Doppler spread (DS), frequency correlation function (FCF), and delay spread (RS). The non-stationarity in the time, space, and frequency domain is captured. It demonstrates that the airspeed, density of scatterers within clusters, and carrier frequency have an impact on statistical properties. Furthermore, twin clusters have more flexible spatial characteristics with lower power than single clusters. These conclusions can provide assistance and reference for the design and deployment of 6G UAV communication systems.
A Negative Emotion Recognition System with Internet of Things-Based Multimodal Biosignal Data
Authors: Ham Seung-Mi , Lee Hye-Min , Lim Jae-Hyun , Seo Jeongwook
Published: October 2023

Previous studies to recognize negative emotions for mental healthcare have used heavy equipment directly attaching electroencephalogram (EEG) electrodes to the head, and they have proposed binary classification methods to identify negative emotions. To tackle this problem, we propose a negative emotion recognition system to collect multimodal biosignal data such as five EEG signals from an EEG headset and heart rate, galvanic skin response, and skin temperature from a smart band for classifying multiple negative emotions. This consists of an Android Internet of Things (IoT) application, a oneM2M-compliant IoT server, and a machine learning server. The Android IoT application uploads the biosignal data to the IoT server. By using the biosignal data stored in the IoT server, the machine learning server recognizes the negative emotions of disgust, fear, and sadness using a multiclass support vector machine (SVM) model with a radial basis function kernel. The experimental results demonstrate that the multimodal biosignal data approach achieves 93% accuracy. Moreover, when considering only data from the smart band, the system achieved 98% accuracy by optimizing the hyperparameters of the multiclass SVM model. Based on these results, we plan to develop a metaverse system that detects and expresses negative emotions in real time.
Physiological and Biochemical Effects of Exogenous Calcium on Camellia oleifera Abel under Drought Stress
Authors: Zhang Tao , Liu Caixia , Chen Yongzhong , Xu Yanming , Tang Wei , Chen Longsheng , Li Ze
Published: October 2023

High temperature and drought are the main factors restricting the increase in Camellia oleifera Abel production that can seriously harm its physiological and biochemical functions. This study aimed to explore the effect by which Ca2+ enhances the drought resistance of C. oleifera. Four-year-old potted plants of the ‘Xianglin 210’ variety were treated with four levels of CaCl2 solution (0, 10, 20, and 40 mmol/L) and subjected to natural drought stress. The drought levels were categorized as CS (non-drought), LD (light drought), MD (moderate drought), and SD (severe drought). The effects of drought stress on the growth and physiological-biochemical characteristics of C. oleifera were measured. Under drought stress, spraying CaCl2 solution on leaves could significantly increase the net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs), endogenous hormone indoleacetic acid (IAA) content, abscisic acid (ABA) content, and gibberellic acid (GA) content in organs and tissues; enhance the superoxide dismutase (SOD) activity; and decrease the concentration of intercellular carbon dioxide (Ci) and number of dehiscence fruits. Spraying 10 mmol/L CaCl2 effectively alleviates the damage of drought stress to tung oil trees. The study provides references for alleviating drought stress and increasing economic benefits in C. oleifera production.
Responses of Stream Water Temperature to Water Levels in Forested Catchments of South Korea
Authors: Nam Sooyoun , Lim Honggeun , Choi Byoungki , Li Qiwen , Moon Haewon , Choi Hyung Tae
Published: October 2023

Event flow characteristics were evaluated based on temperature and level of stream water in 22 forested catchments (area: 13.2–281.4 ha) to investigate sustainable flood management measures. Temperature and stream water levels were during 346 rainfall events in the summer season (July–September) from 2020 to 2022. Rising stream water levels responded to falling stream water temperature between ≤100 and >100 ha forested catchments in two types of time of concentration. Stream water temperature decreased by 3.0 °C when the stream water level increased by up to 0.9 m during rainfall events. Falling stream water temperature at two types of time of concentration was negatively correlated with total precipitation and rising stream water level. Based on the relatively high value of regression and cumulative frequency distribution, the estimated rising stream water level was appropriate in small catchments (≤100 ha) when the stream water temperature decreased, and the stream water level increased during rainfall events. Rising stream water levels and falling stream water temperatures are responses to catchment-scale effects, which are influenced by the nature and rapidity of the hydrological responses. Therefore, the results of the present study indicate that spatial and temporal differences in thermal responses of stream water temperature to water levels were controlled by catchment-scale effects under rapidly changing rainfall.
The Effect of Curcin Protein and Jatropha Plantation on Soil Fungi
Authors: Lai Zhiping , Zhang Bingbing , Niu Xianfei , Ma Rui , Wang Ting , Cheng Cheng , Ren Yingying , Wang Xueying , Hu Na , Jiang Nan , Xu Ying
Published: October 2023

Jatropha curcas is widely planted as a highly drought-resistant biodiesel feedstock. Curcin protein is one of the Jatropha ribosomal inactivation proteins with broad-spectrum antifungal activity that may enter the soil ecosystem as a result of large-scale Jatropha cultivation and affect fungi and various enzymatic activities in the soil. In this research, the influence of curcin protein and Jatropha planting on soil fungi was investigated, and the levels of curcin in various tissues and organs of Jatropha were measured with an enzyme-linked immunosorbent assay. It was found that the content of curcin in seed kernels reaches 2 mg/g, which is much higher than that in other tissues. After the seeds have fallen into the soil, the level of curcin in the soil rises rapidly, reaching 59.22 µg/g soil and 67.49 µg/g soil in different soil samples, respectively. It then falls by more than 99% within six days. High-throughput sequencing technology was used to study the soils treated with different concentrations of curcin, and the results of the soil fungal alpha diversity index analysis showed that the fungal communities did not change significantly, but the abundance of each fungal community changed significantly. The degree of influence of different concentrations of curcin treatment on the abundance of the soil dominant fungal community were investigated for concentrations of 0.5 μg/g, 50 μg/g and 5 μg/g, and showed that concentrations of 0.5 μg/g and 50 μg/g are more likely to change fungal community structure in soil, and with the increasing extension of the treatment time, they may be detrimental to the conservation of soil ecosystems. Internal transcribed spacer (ITS) sequencing of soil fungi from Jatropha planted and unplanted areas in four regions with different climate types showed that Jatropha planting significantly altered the soil fungal communities in each region. There was a negative impact on soil fungal communities in tropical maritime monsoon and subtropical dry and hot monsoon climates, while a positive impact was observed in subtropical monsoon and tropical highland monsoon climates due to Jatropha cultivation. In conclusion, Jatropha plantations and curcin protein have an impact on soil fungi and thereby affect the ecological system of the soil.
Economic Costs of Residential Fires: A Systematic Review
Authors: Rahman Fahmida Saadia , Tannous Wadad Kathy , Avsar Gulay , Agho Kingsley Emwinyore , Ghassempour Nargess , Harvey Lara A.
Published: October 2023

Globally, most fire-related deaths and injuries occur in residential areas. The aim of this systematic review is to report on the economic costs of residential fires from a societal perspective. Five databases (MEDLINE, EMBASE, EconLit, CINAHL, and Scopus) and grey literature were searched to identify studies that report economic or societal costs of residential fires with data from 1978 to 2021. There were no restrictions on study design. A narrative synthesis was undertaken based on the societal and economic costs reported for each included study. Seven studies from the United States, Canada, Australia, and Kuwait reported costs of residential fires. The costs of injuries and deaths were between USD 12 million and USD 5 billion, and between USD 75 million and USD 26 billion, respectively. The costs of treatment ranged from USD 0.3 million to USD 551 million, lost productivity from USD 12 million to USD 4 billion, and property damage from USD 8 million to USD 10 billion. This systematic review provides the most comprehensive evidence to date on the economic costs of residential fires. This study would offer insights into the effects of residential fires on diverse economic agents and aid in community fire prevention messaging and incentives.
Developing the Urban Fire Safety Co-Management System in China Based on Public Participation
Authors: Liu Jida , Ma Ruining , Song Yuwei , Dong Changqi
Published: October 2023

The new situations, problems, and challenges facing urban fire safety work are gradually increasing in China, so innovating urban fire safety governance modes is an urgent task. In the fire management practice of the Chinese government, the establishment of an urban fire safety co-management system is an important measure for aggregating fire safety management resources and improving the level of urban fire safety prevention, as well as control. In order to reveal and clarify the interacting relationships and influencing mechanisms among multiple subjects in an urban fire safety co-management system, we constructed an urban fire safety co-management game model comprising fire supervision departments, production management units, and the public based on evolutionary game theory. The stability of the urban fire safety co-management game system is explored from the perspective of game subjects. The influencing factors of strategy selection between game subjects in the game system were investigated using numerical simulation analysis. The research results show that elevating the informatization level of co-management, the risk perception level of the public, and the disclosure level of fire safety information are conducive to stimulating the public’s positivity to participate in co-management. Strengthening the accountability of the superior government is conducive to ensuring the supervision level of fire supervision departments. The above measures have positive value for optimizing China’s urban fire safety co-management systems, establishing urban fire safety management synergy, and ensuring the stability of social fire safety situations.
Hereditary Thrombotic Thrombocytopenic Purpura
Authors: Nusrat Sanober , Beg Kisha , Khan Osman , Sinha Arpan , George James
Published: October 2023

Hereditary thrombotic thrombocytopenic purpura (hTTP), also known as Upshaw–Schulman syndrome, is a rare genetic disorder caused by mutations in the ADAMTS13 gene that leads to decreased or absent production of the plasma von Willebrand factor (VWF)-cleaving metalloprotease ADAMTS13. The result is circulating ultra-large multimers of VWF that can cause microthrombi, intravascular occlusion and organ damage, especially at times of turbulent circulation. Patients with hTTP may have many overt or clinically silent manifestations, and a high index of suspicion is required for diagnosis. For the treatment of hTTP, the goal is simply replacement of ADAMTS13. The primary treatment is prophylaxis with plasma infusions or plasma-derived factor VIII products, providing sufficient ADAMTS13 to prevent acute episodes. When acute episodes occur, prophylaxis is intensified. Recombinant ADAMTS13, which is near to approval, will immediately be the most effective and also the most convenient treatment. In this review, we discuss the possible clinical manifestations of this rare disease and the relevant differential diagnoses in different age groups. An extensive discussion on prophylaxis and treatment strategies is also presented. Unique real patient cases have been added to highlight critical aspects of hTTP manifestations, diagnosis and treatment.
Quantitative Assessment of Balance Function Characteristics in Older Patients with Orthostatic Hypotension
Authors: Cui Yao , Liu Bo , Zhou Jian , Liu Qian , Ye Hui
Published: October 2023

Background: Orthostatic hypotension (OH) is a common blood pressure abnormality in older adults that makes them prone to balance disorders and falls. The maintenance of balance relies on a complex regulatory system. The use of computerized dynamic posturography (CDP) can provide a quantitative evaluation of balance function. The objective of this study was to utilize CDP to measure balance indicators in older individuals with OH. Methods: A total of 303 older adults were divided into an OH group of 91 and a non-OH group of 212. Various factors, including chronic diseases, medication history, laboratory tests, and balance indicators, were compared between the two groups. Results: ① Participants with OH had more chronic diseases, including coronary heart disease (p = 0.049) and a history of falls (p < 0.001), than those without OH. A history of multiple medications in the OH group was significantly more likely than in the non-OH group (p = 0.006). ② There was a significant reduction in the composite equilibrium score (SOT-COM) (p < 0.001), vision ratio score (VIS) (p < 0.001), vestibular ratio score (VEST) (p < 0.001), and directional control (DCL) (p = 0.028) in the OH group. ③ A logistic regression analysis revealed that SOT-COM was a significant independent factor associated with OH. The area under the curve (AUC) of SOT-COM was 0.833 (95% confidence interval: 0.778–0.887, p < 0.001), with a sensitivity of 0.826 and a specificity of 0.759. Conclusions: This study demonstrates that older individuals with OH are more prone to falls, due to decreased sensory integration for balance.
The Association between Oral Health Management and the Functional Independence Measure Scores at the Time of Admission of Inpatients to a Convalescent Hospital
Authors: Hara Ryuzo , Todayama Naoki , Tabata Tomohiro , Kuwazawa Miki , Mukai Tomoko , Hatanaka Yukiko , Hironaka Shouji , Kawate Nobuyuki , Furuya Junichi
Published: October 2023

Many older patients admitted to convalescent hospitals present with impaired oral function, which is an important function of daily life. This study aimed to investigate the actual status of the oral healthcare needs of inpatients in a convalescent hospital and to clarify the relationship between the level of independence at admission and the oral function. The participants were 147 patients (94 males, 53 females, mean age: 74.6 ± 13.1 years) who received dental visits during their stay at a convalescent hospital. Information regarding general and oral health was extracted from medical records. Most patients were found to have low Functional Independence Measure motor scores, and approximately 70% had some form of oral intake, but approximately 80% had nutritional problems. The mean number of present and functional teeth were 16.6 and 20.8, respectively, and 65% of patients lost molar occlusal support. Multiple regression analysis showed significant positive correlations of high functional independence measure with age, eating status, nutritional status, and the number of functional teeth. This implied that oral health management is important for patients in a convalescent hospital and that enhancing oral health may be related to improved eating textures and better nutritional status.
Israeli and Palestinian Settler Colonialism in New Media: The Case of Roots
Authors: Pycińska Magdalena
Published: October 2023

Israeli settler colonialism, in time, became highly linked to the idea of a state, culminating in an institution that defends the past, present, and future practises maintaining the relations between the “native” and “settlers”. Settler colonial ideas and practises sustaining binary opposition between the “native” and the “settler” are reproduced not only by Israeli state broadcasters, but also by settler colonial social media. This article proposes media analysis that goes beyond the usual national and conflict narrative and links “settler colonial common sense” with social media impacts and state ideas/sovereign ideas of property that strive to eliminate native people or transfer them outside Israel’s perceived land ownership and sovereignty. This article also shows how Israeli settler colonial politics and narratives are supported by other settler colonial states (especially the United States). New media and settler common sense cannot be disassociated from the Israeli state and global politics, even though some settlers may have their own strategies regarding the relations with native Palestinians. The State of Israel, through massive surveillance technologies and support from other states that view militarisation and population management as crucial to maintaining its power, holds a great deal of influence over how it frames the “conflict” with Palestinians. We witness how both state violence and institutionalised Jewish privilege are recreated on the ground and globally through the new media. This issue is analysed through the “Roots” (a grassroots movement for understanding among Israelis and Palestinians) case study.
Speaking of Sex: Critical Reflections for Forensic Anthropologists
Authors: Flaherty Taylor M. , Johnson Liam J. , Woollen Katharine C. , Lopez Dayanira , Gaddis Katherine , Horsley SaMoura L. , Byrnes Jennifer F.
Published: October 2023

Forensic anthropologists have a responsibility to appropriately relay information about a decedent in medicolegal reports and when communicating with the public. The terms ‘sex’ and ‘sex estimation’ have been applied with numerous, inconsistent definitions under the guise that sex—a broad, complex concept—can be reduced to a female/male binary. This binary does not reflect biocultural realities and harms those whose bodies do not meet social expectations of maleness or femaleness. The University of Nevada, Las Vegas’ Forensic Anthropology and Bioarchaeology Laboratory (UNLV FAB Lab) advocates for the use of the term ‘assigned sex at birth’ (ASAB) to highlight that binary sex is not biologically inherent to the body, but rather, assigned by society. Additionally, we call for the use of disclaimers in case reports to denote the limitations of ASAB estimation methods, the differentiation between those with mixed trait expression (i.e., indeterminate) and those on whom an ASAB analysis cannot be performed (i.e., unknown), and the included consideration of gender in forensic anthropology research and case reports. Such applications challenge biological normalcy, allowing forensic anthropologists to actively advocate for those whose bodies do not meet biocultural expectations.
A Modified Method for Transient Transformation via Pollen Magnetofection in Lilium Germplasm
Authors: Zhang Mingfang , Ma Xu , Jin Ge , Han Dongyang , Xue Jing , Du Yunpeng , Chen Xuqing , Yang Fengping , Zhao Chunli , Zhang Xiuhai
Published: October 2023

Lily (Lilium spp.) is a popular ornamental plant. Traditional genetic transformation methods have low efficiency in lily, thus development of a high-efficiency genetic transformation system is important. In this study, a novel transient transformation method involving pollen magnetofection was established and optimized pollen viability, and exogenous gene expression in magnetofected pollen and that of different germplasm were assessed. The highest germination percentage of Lilium regale pollen was 85.73% in medium containing 100 g/L sucrose, 61.5 mg/L H3BO3, and 91.5 mg/L CaCl2. A 1:4 ratio of nanomagnetic beads to DNA plasmid and transformation time of 0.5 h realized the highest transformation efficiency (88.32%). The GFP activity in transformed pollen averaged 69.66%, while that of the control pollen was 0.00%. In contrast to the control, transgenic seedlings obtained by pollination with magnetofected pollen showed strong positive GUS activity with 56.34% transformation efficiency. Among the lily germplasm tested, ‘Sweet Surrender’ and L. leucanthum had the highest transformation efficiency (85.80% and 54.47%), whereas L. davidii var. willmottiae was not successfully transformed. Transformation efficiency was positively correlated with pollen equatorial diameter and negatively correlated with polar axis/equatorial diameter ratio. The results suggest that pollen magnetofection-mediated transformation can be applied in Lilium but might have species or cultivar specificity.
Predictive Biomarkers for Immunotherapy in Gastric Cancer: Current Status and Emerging Prospects
Authors: Hou Wanting , Zhao Yaqin , Zhu Hong
Published: October 2023

Gastric cancer presents substantial management challenges, and the advent of immunotherapy has ignited renewed hope among patients. Nevertheless, a significant proportion of patients do not respond to immunotherapy, and adverse events associated with immunotherapy also occur on occasion, underscoring the imperative to identify suitable candidates for treatment. Several biomarkers, including programmed death ligand-1 expression, tumor mutation burden, mismatch repair status, Epstein–Barr Virus infection, circulating tumor DNA, and tumor-infiltrating lymphocytes, have demonstrated potential in predicting the effectiveness of immunotherapy in gastric cancer. However, the quest for the optimal predictive biomarker for gastric cancer immunotherapy remains challenging, as each biomarker carries its own limitations. Recently, multi-omics technologies have emerged as promising platforms for discovering novel biomarkers that may help in selecting gastric cancer patients likely to respond to immunotherapy. The identification of reliable predictive biomarkers for immunotherapy in gastric cancer holds the promise of enhancing patient selection and improving treatment outcomes. In this review, we aim to provide an overview of clinically established biomarkers of immunotherapy in gastric cancer. Additionally, we introduce newly reported biomarkers based on multi-omics studies in the context of gastric cancer immunotherapy, thereby contributing to the ongoing efforts to refine patient stratification and treatment strategies.
Uridine Diphosphate Glycosyltransferases (UGTs) Involved in the Carotenoid-Based Body Color Difference between Tetranychus cinnabarinus (Red) and Tetranychus urticae (Green)
Authors: Xu Zhifeng , Lin Ting , Wang Tongyang , Hu Yuan , Shen Guangmao , Feng Kaiyang , Zhang Ping , He Lin
Published: October 2023

It has long been disputed whether Tetranychus cinnabarinus and Tetranychus urticae belong to the same genus, with T. cinnabarinus regarded as a red form of T. urticae. However, it is unclear why T. urticae and T. cinnabarinus have different body colors. Since carotenoids are responsible for the color of many organisms, the carotenoid profiles of T. cinnabarinus and T. urticae were compared by HPLC. There was no difference in carotenoid type, but T. cinnabarinus contained significantly more neoxanthin, astaxanthin, α-carotene, β-carotene, and γ-carotene, which may contribute to the deep red color. The transcriptome sequencing of both species identified 4079 differentially expressed genes (DEGs), of which 12 were related to carotenoid metabolism. RNA interference (RNAi) experiments demonstrated that silencing seven of these DEGs resulted in the different accumulation of carotenoid compounds in T. cinnabarinus and T. urticae. In addition, the body of T. urticae turned yellow after two days of feeding with UGT double-stranded RNAs and β-UGT small interfering RNAs. In conclusion, differences in the carotenoid profiles of T. urticae and T. cinnabarinus may be responsible for the different body colors.
Robot-Assisted and Manual Cochlear Implantation: An Intra-Individual Study of Speech Recognition
Authors: Maheo Clémentine , Marie Antoine , Torres Renato , Archutick Jerrid , Leclère Jean-Christophe , Marianowski Remi
Published: October 2023

Cochlear implantation (CI) allows rehabilitation for patients with severe to profound hearing impairment. Although the use of a robotic assistant provides technical assistance to the surgeon, the assessment of the impact of its use on auditory outcomes remains uncertain. We aim to compare the hearing results of patients who underwent bilateral cochlear implantation; one side was performed with manual insertion and the other side with robot-assisted insertion. The electrode array intrascalar positioning and the surgery duration were also studied. This retrospective intra-individual study involved 10 patients who underwent bilateral cochlear implantation. The study included two infants and eight adults. The unique composition of this cohort enabled us to utilize each patient as their own control. Regarding speech disyllabic recognition, pure tone average, ECAP, ratio of array translocation, basilar membrane rupture, and percentage of translocated electrodes, there was no difference between manual and robot-assisted CI groups. This study is the first to compare intra-individual hearing performance after cochlear implantation, either manually or robot-assisted. The number of patients and the time delay between manual and robotic implantation may have led to a lack of power, but there was no apparent difference in hearing performance between manual and robotic implantation.