bluuebunny/crossref_metadata_2025 huggingface dataset:
Predictive Model of Gait Recovery at One Month after Hip Fracture from a National Cohort of 25,607 Patients: The Hip Fracture Prognosis (HF-Prognosis) Tool
Authors: González de Villaumbrosia Cristina , Sáez López Pilar , Martín de Diego Isaac , Lancho Martín Carmen , Cuesta Santa Teresa Marina , Alarcón Teresa , Ojeda Thies Cristina , Queipo Matas Rocío , González-Montalvo Juan ,   on behalf of the Participants in the Spanish National Hip Fracture Registry
Published: April 2021

The aim of this study was to develop a predictive model of gait recovery after hip fracture. Data was obtained from a sample of 25,607 patients included in the Spanish National Hip Fracture Registry from 2017 to 2019. The primary outcome was recovery of the baseline level of ambulatory capacity. A logistic regression model was developed using 40% of the sample and the model was validated in the remaining 60% of the sample. The predictors introduced in the model were: age, prefracture gait independence, cognitive impairment, anesthetic risk, fracture type, operative delay, early postoperative mobilization, weight bearing, presence of pressure ulcers and destination at discharge. Five groups of patients or clusters were identified by their predicted probability of recovery, including the most common features of each. A probability threshold of 0.706 in the training set led to an accuracy of the model of 0.64 in the validation set. We present an acceptably accurate predictive model of gait recovery after hip fracture based on the patients’ individual characteristics. This model could aid clinicians to better target programs and interventions in this population.
Association between Moving to a High-Volume Hospital in the Capital Area and the Mortality among Patients with Cancer: A Large Population-Based Cohort Study
Authors: Choi Jung-kyu , Kim Se-Hyung , Park Myung-Bae
Published: April 2021

This study aimed to identify the association between moving to a high-volume hospital and the mortality of patients with cancer living in the district. The study population comprised participants diagnosed with cancer within the past nine years (2004–2012). The final sample included 8197 patients with cancer, 3939 were males (48.1%), and 4258 were females (51.9%). A Cox proportional hazard model was used to estimate the hazard ratio (HR) for death. Confounding variables including sex, age, type of social security, income level, disability, and utilization volume were incorporated into the model. Among patients with cancer living in the district, 2874 (35.1%) used healthcare services in Seoul. About 10% (n = 834) of patients died during the follow-up period. The HR for death in females (HR: 0.68, 95% CI: 0.58–0.81) was lower than that in males. Additionally, the HR for the death of patients using healthcare services in Seoul (HR: 1.30, 95% CI: 1.11–1.53) was higher than those patients who did not use healthcare services in Seoul. Among patients utilizing services in the province, wealthier patients’ survival probability was significantly higher than that of others. The cause of income differences should be identified, and accessibility to medical use of low-income families should be enhanced to prevent mortality of patients from cancer disparities.
Secondary School Nutrition Policy Compliance in Ontario and Alberta, Canada: A Follow-Up Study Examining Vending Machine Data from the COMPASS Study
Authors: Vine Michelle M. , Vermeer Julianne , Romano Leonardo , Harrington Daniel W. , Butler Alexandra E. , Patte Karen A. , Godin Katelyn M. , Leatherdale Scott T.
Published: April 2021

(1) Objective: To longitudinally assess food and beverages sold in vending machines in secondary schools (grades 9–12) participating in the COMPASS study (2015/2016 and 2018/2019) and (2) to examine if patterns and trends observed in previous years (2012/2013 to 2014/2015) are consistent with lack of policy compliance in Ontario and Alberta, Canada. (2) Methods: Policy compliance was assessed through comparing nutritional information on drink (e.g., sports drinks) and snack (e.g., chocolate bars) products in vending machines to Policy and Program Memorandum (P/PM) 150 in Ontario (required policy) and the Alberta Nutrition Guidelines for Children and Youth (recommended policy). Longitudinal results and descriptive statistics were calculated. (3) Results: Longitudinal results indicate that between Y4 (2015/2016) and Y7 (2018/2019), snack and drink vending machines remained mostly non-compliant in Ontario and Alberta, with a small proportion of Ontario drink machines changing from non-compliant to compliant. At the school level, descriptive results indicate the proportion of Ontario schools with policy-compliant snack and drink machines decreased between Y4 and Y7. Alberta schools were non-compliant for drink and snack machines. (4) Conclusions: Secondary schools continue to be non-compliant with provincial policies. School nutrition policies need to be simplified in order to make it easier for schools to be compliant. Enforcement of compliancy is also an area that deserves consideration.
The Acute Post-Activation Performance Enhancement of the Bench Press Throw in Disabled Sitting Volleyball Athletes
Authors: Krzysztofik Michal , Matykiewicz Patryk , Celebanska Diana , Jarosz Jakub , Gawel Eliza , Zwierzchowska Anna
Published: April 2021

The purpose of the present study was to examine the acute effects of the bench press exercise with predetermined velocity loss percentage on subsequent bench press throw (BPT) performance with raised legs or feet on the floor among disabled, sitting volleyball players. Twelve elite sitting volleyball athletes (age = 33 ± 9 years; body mass = 84.7 ± 14.7 kg; relative bench press maximum strength = 1.0 ± 0.3 kg/body mass) took part in this study. The experiment was performed following a randomized crossover design, where each participant performed a single set of bench press with a 60% one-repetition maximum (1RM) to a 10% decrease of mean bar velocity as a conditioning activity (CA). The BPT with a 60%1RM was performed to assess changes in peak power (PP), peak velocity (PV) before and after the CA. The differences between analyzed variables before and after the CA were verified using two-way repeated-measures ANOVA (condition × time; 2 × 2). The ANOVA showed a significant main effect of time for peak bar velocity (p = 0.03; η2 = 0.312) and peak power output (p = 0.037; η2 = 0.294). The post hoc comparison showed a significant increase in post-CA peak bar velocity and peak power for raised legs condition in comparison with pre-CA value (p = 0.02, p = 0.041, respectively). The present study showed that the subsequent BPT performed with raised legs could be enhanced by the bench press with a 60% 1RM to a 10% mean bar velocity decrease as a CA among disabled sitting volleyball players. Therefore, athletes and coaches can consider performing a bench press throw with raised legs without compromising performance.
Development of the Conceptualization of Pain Questionnaire: A Measure to Study How Children Conceptualize Pain
Authors: Salvat Isabel , Adillón Cristina , Andrés Eva Maria , Monterde Sonia , Miró Jordi
Published: April 2021

(1) Background: Research has shown that thoughts about pain are important for the management of chronic pain in children. In order to monitor changes in thoughts about pain over time and evaluate the efficacy of treatments, we need valid and reliable measures. The aims of this study were to develop a questionnaire to assess a child’s concept of pain and to evaluate its psychometric properties; (2) Methods: This is a cross-sectional, two-phase, mixed-method study. A total of 324 individuals aged 8 to 17 years old responded to the newly created questionnaire. The Conceptualization of Pain Questionnaire (COPAQ) was calibrated using the Rasch model. The chi-square test was used for the fit statistics. Underfit and overfit of the model were determined and a descriptive analysis of infit and outfit was conducted to identify who responded erratically. Internal consistency was measured using the Person Separation Index (PSI); (3) Results: Fit to the Rasch model was good. Suitable targeting indicated which items were simple to answer; Person Fit identified 9.56% children who responded erratically; PSI = 0.814; (4) Conclusions: The findings suggest that COPAQ is a measure of a child’s concept of pain that is easy to administer and respond to. It has a good fit and a good internal consistency.
Falsified Drugs in the Opinion of Patients Diagnosed with Cardiovascular Diseases—Nationwide and Cross-Sectional Study on the Example of EU-Member Country
Authors: Świeczkowski Damian , Zdanowski Szymon , Merks Piotr , Jaguszewski Miłosz
Published: April 2021

Background: In light of a falsified medications pandemic, understanding the patient perspective on falsified medicines is warranted. Our study aimed to investigate the perspectives regarding falsified medicines among patients with cardiovascular diseases. Methods: Computer-assisted telephone interviews were conducted based on a questionnaire: (i) Respondents suffering from cardiovascular diseases and (ii) respondents not being chronically ill. Only participants below 50 years of age were included. Results: We enrolled 1200 respondents total, 800 in the study group and 400 in the control group (in cooperation with a professional public opinion research center). The vast majority of participants agreed that community pharmacies are the only place that ensures the secure purchasing of non-falsified drugs (67.01% study group and 65.25% control group; p < 0.01). The majority of respondents were convinced that purchasing medications on the Internet is associated with a higher risk of receiving falsified drugs. Patients diagnosed with cardiovascular diseases and those with “non-satisfactory financial situation” had significantly decreased likelihoods of obtaining a high score in general knowledge on falsified medications (OR = 0.64 and OR = 0.58, respectively). Conclusions: Awareness of the risks associated with falsified drugs among patients with cardiovascular diseases remains high but still insufficient.
Environmental Detection of SARS-CoV-2 Virus RNA in Health Facilities in Brazil and a Systematic Review on Contamination Sources
Authors: Vicente Vania Aparecida , Lustosa Bruno Paulo Rodrigues , Grisolia Maria Eduarda , Pavini Beato Caroline , Balsanelli Eduardo , de Souza Gubert Fruet Viviane , Bordignon Nogueira Meri , Raboni Sonia Maria , Carvalho Katherine Athayde Teixeira , Flôr Izadora Cervelin , Ferreira Voidaleski Morgana , Etchepare Ramiro Gonçalves , Meis Jacques F. , Soccol Vanete Thomaz , Souza Emanuel Maltempi
Published: April 2021

SARS-CoV-2 environmental monitoring can track the rate of viral contamination and can be used to establish preventive measures. This study aimed to detect by RT-PCR the presence of SARS-CoV-2 from inert surface samples in public health settings with a literature review about surface contamination and its burden on spread virus. Samples were collected from health settings in Curitiba, Brazil, between July and December 2020. A literature review was conducted using PRISMA. A total of 711 environmental surface samples were collected from outpatient areas, dental units, doctors’ offices, COVID-19 evaluation areas, and hospital units, of which 35 (4.9%) were positive for SARS-CoV-2 RNA. The frequency of environmental contamination was higher in primary care units than in hospital settings. The virus was detected on doctors’ personal items. Remarkably, the previously disinfected dental chair samples tested positive. These findings agree with those of other studies in which SARS-CoV-2 was found on inanimate surfaces. Detection of SARS-CoV-2 RNA on surfaces in public health settings, including those not meant to treat COVID-19, indicates widespread environmental contamination. Therefore, the intensification of disinfection measures for external hospital areas may be important for controlling community COVID-19 dissemination.
Quantitative Models for Prediction of Cumulative Trauma Disorders Applied to the Maquiladora Industry
Authors: Cázares-Manríquez Melissa Airem , Camargo-Wilson Claudia , Vardasca Ricardo , García-Alcaraz Jorge Luis , Olguín-Tiznado Jesús Everardo , López-Barreras Juan Andrés , García-Rivera Blanca Rosa
Published: April 2021

Temperature gradient changes on the surface of the skin or in the middle of the body are signs of a disease. The aim of this study is to develop quantitative models for the prediction of cumulative trauma disorders (CTDs) arising from highly repetitive activities, considering risk factors, such as age, gender, body mass index (BMI), blood pressure (BP), respiratory rate (RR), and heart rate, to prevent injuries in manufacturing factory operators. This research involved 19 individuals from the area of sanding and 14 individuals from the area of tolex in manufacturing factories who had their vital signs and somatometry taken, as well as thermal images of their hands in the dorsal and palmar areas; an evaluation by the OCRA method was also applied. Factors such as BP and heart rate were determined to significantly influence the injuries, but no strong association with BMI was found. Quadratic regression models were developed, the estimates of which were adequately adjusted to the variable (R2 and R2 adjusted > 0.70). When integrating the factors of the OCRA method to the generated models, a better fit was obtained (R2 and adjusted R2 > 0.80). In conclusion, the participants who present levels out of the normal range in at least one of the factors have high probabilities of developing injuries in their wrists.
Factors Associated with Happiness among Malaysian Elderly
Authors: Shah Shamsul Azhar , Safian Nazarudin , Ahmad Saharuddin , Wan Ibadullah Wan Abdul Hannan , Mohammad Zulkefley bin , Nurumal Siti Rohani , Mansor Juliana , Addnan Mohd Fairuz , Shobugawa Yugo
Published: April 2021

Happiness is an essential component to experience healthy ageing. Hence, understanding the factors that contribute to happiness is important. This study aimed to determine the factors associated with happiness among the elderly population in Malaysia. In this study, 1204 respondents were recruited from urban and rural areas in Selangor. A face-to-face interview was conducted using the Bahasa Malaysia version of the Japan Gerontological Evaluation Study questionnaire. The inclusion criteria include Malaysians who are 60-years old and above and can converse in the Malaysian language. Those who encounter less than seven scores for the Abbreviated Mental Test were excluded from the study. Among the 1204 respondents, 953 (79.2%) were happy. Sociodemographic characteristics showed that being a men, age of 60 to 74 years, and living in urban areas were significantly associated with happiness. A logistic regression model showed that locality (aOR 1.61), income category (Bottom 40% aOR 0.49; Middle-class group 40% aOR 1.40), social engagement (active aOR 1.77; less active aOR 1.25), receiving emotional support (aOR 2.11) and handgrip strength (aOR 1.02) were significantly associated with happiness. Thus, ensuring the elderly population in receiving emotional support and active social engagement among them can enhance their happiness level.
Predicting the Dynamics of the COVID-19 Pandemic in the United States Using Graph Theory-Based Neural Networks
Authors: Davahli Mohammad Reza , Fiok Krzysztof , Karwowski Waldemar , Aljuaid Awad M. , Taiar Redha
Published: April 2021

The COVID-19 pandemic has had unprecedented social and economic consequences in the United States. Therefore, accurately predicting the dynamics of the pandemic can be very beneficial. Two main elements required for developing reliable predictions include: (1) a predictive model and (2) an indicator of the current condition and status of the pandemic. As a pandemic indicator, we used the effective reproduction number (Rt), which is defined as the number of new infections transmitted by a single contagious individual in a population that may no longer be fully susceptible. To bring the pandemic under control, Rt must be less than one. To eliminate the pandemic, Rt should be close to zero. Therefore, this value may serve as a strong indicator of the current status of the pandemic. For a predictive model, we used graph neural networks (GNNs), a method that combines graphical analysis with the structure of neural networks. We developed two types of GNN models, including: (1) graph-theory-based neural networks (GTNN) and (2) neighborhood-based neural networks (NGNN). The nodes in both graphs indicated individual states in the United States. While the GTNN model’s edges document functional connectivity between states, those in the NGNN model link neighboring states to one another. We trained both models with Rt numbers collected over the previous four days and asked them to predict the following day for all states in the United States. The performance of these models was evaluated with the datasets that included Rt values reflecting conditions from 22 January through 26 November 2020 (before the start of COVID-19 vaccination in the United States). To determine the efficiency, we compared the results of two models with each other and with those generated by a baseline Long short-term memory (LSTM) model. The results indicated that the GTNN model outperformed both the NGNN and LSTM models for predicting Rt.
How Self-Control Predicts Moral Decision Making: An Exploratory Study on Healthy Participants
Authors: Lucifora Chiara , Martino Gabriella , Curcuruto Anna , Salehinejad Mohammad Ali , Vicario Carmelo Mario
Published: April 2021

Research on moral reasoning calls into question self-control, which encompasses impulsivity, compulsivity, and inhibitory control. However, a thorough investigation exploring how these three dimensions can affect moral reasoning in response to different scenarios is unavailable. We addressed this topic by testing the predictive role of these three dimensions of self-control on appraisals for ethical violations related with different types of scenarios. Overall, our results suggest that all three dimensions of self-control are involved in moral reasoning, depending on the type of appraisal and provided moral scenarios.
Geographic Factors Associated with Poorer Outcomes in Patients Diagnosed with COVID-19 in Primary Health Care
Authors: Magallón-Botaya Rosa , Oliván-Blázquez Bárbara , Ramírez-Cervantes Karen Lizzette , Méndez-López-de-la-Manzanara Fátima , Aguilar-Palacio Isabel , Casajuana-Closas Marc , Andrés-Esteban Eva
Published: April 2021

Background: The prognosis of older age COVID-19 patients with comorbidities is associated with a more severe course and higher fatality rates but no analysis has yet included factors related to the geographical area/municipality in which the affected patients live, so the objective of this study was to analyse the prognosis of patients with COVID-19 in terms of sex, age, comorbidities, and geographic variables. Methods: A retrospective cohort of 6286 patients diagnosed with COVID-19 was analysed, considering demographic data, previous comorbidities and geographic variables. The main study variables were hospital admission, intensive care unit (ICU) admission and death due to worsening symptoms; and the secondary variables were sex, age, comorbidities and geographic variables (size of the area of residence, distance to the hospital and the driving time to the hospital). A comparison analysis and a multivariate Cox model were performed. Results: The multivariate Cox model showed that women had a better prognosis in any type of analysed prognosis. Most of the comorbidities studied were related to a poorer prognosis except for dementia, which is related to lower admissions and higher mortality. Suburban areas were associated with greater mortality and with less hospital or ICU admission. Distance to the hospital was also associated with hospital admission. Conclusions: Factors such as type of municipality and distance to hospital act as social health determinants. This fact must be taken account in order to stablish specifics prevention measures and treatment protocols.
The Role of Conspiracy Theories in the Spread of COVID-19 across the United States
Authors: Gu Fu , Wu Yingwen , Hu Xinyu , Guo Jianfeng , Yang Xiaohan , Zhao Xinze
Published: April 2021

The outbreak of coronavirus disease 2019 (COVID-19) inspires various conspiracy theories, which could divert public attention, alter human behaviors, and consequently affect the spread of the pandemic. Here we estimate the relation of the online attention on COVID-19-related conspiracy theories to human mobility, as well as to the numbers of confirmed COVID-19 cases, during 14 March 2020 to 28 August 2020. We observe that the online attention to COVID-19 conspiracy theories is significantly and negatively related to human mobility, but its negative impact is noticeably less than those of the attention to official information and personal protection measures. Since human mobility significantly promotes the spread of COVID-19, the attention to official information and personal protection measures lowers COVID-19 cases by 16.16% and 9.41%, respectively, while attention to conspiracy theories only reduces the COVID-19 cases by 6.65%. In addition, we find that in the states with higher online attention to COVID-19 conspiracy theories, the negative relation of the attention to conspiracy theories is much weaker than that in states where there is less concern about conspiracies. This study stresses the necessity of restricting the online transmission of unfounded conspiracy theories during a pandemic.
Patient Safety Related to Microbiological Contamination of the Environment of a Multi-Profile Clinical Hospital
Authors: Robakowska Marlena , Bronk Marek , Tyrańska-Fobke Anna , Ślęzak Daniel , Kraszewski Jakub , Balwicki Łukasz
Published: April 2021

Nosocomial infections pose a serious burden for hospitals, patients, and the entire society. The aim of the study was to assess the microbiological cleanliness of the hospital environment through quantitative and qualitative analysis of microbiological contamination of air and surfaces in inpatient treatment facilities, based on the example of a large clinical hospital in Poland. Data were collected between 2012 and 2018 in premises of a large teaching hospital in Gdansk using the sedimentation method and the impact method using the Aerideal apparatus (Biomerieux). In the analyzed clinical center, the microbiological cleanliness tests in most of the hospital rooms in the analyzed period showed an acceptable number of saprophytic microorganisms. Of all the tested samples, 1159 (21.8%) were positive, indicating the presence of microorganisms in the tested sample. Species potentially pathogenic for hospital patients were identified, constituting 20.8% of all positive samples (4.6% of all samples). Significantly higher proportion of microorganisms potentially dangerous to patients were isolated from sanitary facilities. Due to the potentially pathogenic microorganisms detected in the tested samples, the authors suggest that in the analyzed hospital, the areas requiring a specific level of microbiological purity should be designated and described, with [specifically] defined cleaning and disinfection protocols.
Effect of an Endurance and Strength Mixed Circuit Training on Regional Fat Thickness: The Quest for the “Spot Reduction”
Authors: Paoli Antonio , Casolo Andrea , Saoncella Matteo , Bertaggia Carlo , Fantin Marco , Bianco Antonino , Marcolin Giuseppe , Moro Tatiana
Published: April 2021

Accumulation of adipose tissue in specific body areas is related to many physiological and hormonal variables. Spot reduction (SR) is a training protocol aimed to stimulate lipolysis locally, even though this training protocol has not been extensively studied in recent years. Thus, the present study sought to investigate the effect of a circuit-training SR on subcutaneous adipose tissue in healthy adults. Methods: Fourteen volunteers were randomly assigned to spot reduction (SR) or to a traditional resistance training (RT) protocol. Body composition via bioimpedance analysis (BIA) and subcutaneous adipose tissue via skinfold and ultrasound were measured before and after eight weeks of training. Results: SR significantly reduced body mass (p < 0.05) and subcutaneous abdominal adipose tissue (p < 0.05). Conclusions: circuit-training SR may be an efficient strategy to reduce in a localized manner abdominal subcutaneous fat tissue depot.
The Key Factors Driving the Development of New Towns by Mother Cities and Regions: Evidence from China
Authors: Zhao Sidong , Zhang Congguo , Qi Junheng
Published: April 2021

As an important carrier of expanded urban spatial growth, new towns have been a “policy tool” for spatial production in the new era and have received long-term and constant attention from circles such as geography, planning, and economics. National new districts constitute a new regional space for China to implement the national strategy and promote the transformation of urban development mode. They are mutually reinforcing with their mother cities and hinterland provinces. Based on the geodetector method, this paper reveals the key factors driving the development of national new districts by mother cities and hinterland provinces and their interaction effects, which provides a basis for municipal and provincial governments to accurately formulate policies to promote the development of new towns by classification. The study shows that, firstly, there are five types of driving factors, that is, all-round driving factors, scale-increasing factors, expansion and quality-improving factors, expertise driving factors, and non-driving factors. The strength and dimension of the driving factors are characterized by prominent heterogeneity; R&D personnel, export and import trade are the key factors to expand the increment, optimize the inventory, and improve the quality; the overall development driving forces are in the order of innovation > opening > industry > investment > population. Secondly, the pairwise interaction between different factors exhibits two-factor enhancement, and the population shows a nonlinear increase in the driving force of investment, openness, and innovation on a provincial scale. Thirdly, according to the driving force of the factors and the interaction between them, suggestions are put forward based on the development stage and key demands for city and provincial governments to make policies for the development of national new districts, to support the establishment of scientific competition and cooperation between new towns and mother cities or regions, and to build a long-term collaborative development mechanism.
PPTPF: Privacy-Preserving Trajectory Publication Framework for CDR Mobile Trajectories
Authors: Yang Jianxi , Dash Manoranjan , Teo Sin G.
Published: April 2021

As mobile phone technology evolves quickly, people could use mobile phones to conduct business, watch entertainment shows, order food, and many more. These location-based services (LBS) require users’ mobility data (trajectories) in order to provide many useful services. Latent patterns and behavior that are hidden in trajectory data should be extracted and analyzed to improve location-based services including routing, recommendation, urban planning, traffic control, etc. While LBSs offer relevant information to mobile users based on their locations, revealing such areas can pose user privacy violation problems. An efficient privacy preservation algorithm for trajectory data must have two characteristics: utility and privacy, i.e., the anonymized trajectories must have sufficient utility for the LBSs to carry out their services, and privacy must be intact without any compromise. Literature on this topic shows many methods catering to trajectories based on GPS data. In this paper, we propose a privacy preserving method for trajectory data based on Call Detail Record (CDR) information. This is useful as a vast number of people, particularly in underdeveloped and developing places, either do not have GPS-enabled phones or do not use them. We propose a novel framework called Privacy-Preserving Trajectory Publication Framework for CDR (PPTPF) for moving object trajectories to address these concerns. Salient features of PPTPF include: (a) a novel stay-region based anonymization technique that caters to important locations of a user; (b) it is based on Spark, thus it can process and anonymize a significant volume of trajectory data successfully and efficiently without affecting LBSs operations; (c) it is a component-based architecture where each component can be easily extended and modified by different parties.
Edge Detection in 3D Point Clouds Using Digital Images
Authors: Dolapsaki Maria Melina , Georgopoulos Andreas
Published: April 2021

This paper presents an effective and semi-automated method for detecting 3D edges in 3D point clouds with the help of high-resolution digital images. The effort aims to contribute towards addressing the unsolved problem of automated production of vector drawings from 3D point clouds of cultural heritage objects. Edges are the simplest primitives to detect in an unorganized point cloud and an algorithm was developed to perform this task. The provided edges are defined and measured on 2D digital images of known orientation, and the algorithm determines the plane defined by the edge on the image and its perspective center. This is accomplished by applying suitable transformations to the image coordinates of the edge points based on the Analytical Geometry relationships and properties of planes in 3D space. This plane inevitably contains the 3D points of the edge in the point cloud. The algorithm then detects and isolates those points which define the edge in the world system. Finally, the goal is to reliably locate the points that describe the desired edge in their true position in the geodetic space, using several constraints. The algorithm is firstly investigated theoretically for its efficiency using simulation data and then assessed under real conditions and under different image orientations and lengths of the edge on the image. The results are presented and evaluated.
Manmade Electromagnetic Fields and Oxidative Stress—Biological Effects and Consequences for Health
Authors: Schuermann David , Mevissen Meike
Published: April 2021

Concomitant with the ever-expanding use of electrical appliances and mobile communication systems, public and occupational exposure to electromagnetic fields (EMF) in the extremely-low-frequency and radiofrequency range has become a widely debated environmental risk factor for health. Radiofrequency (RF) EMF and extremely-low-frequency (ELF) MF have been classified as possibly carcinogenic to humans (Group 2B) by the International Agency for Research on Cancer (IARC). The production of reactive oxygen species (ROS), potentially leading to cellular or systemic oxidative stress, was frequently found to be influenced by EMF exposure in animals and cells. In this review, we summarize key experimental findings on oxidative stress related to EMF exposure from animal and cell studies of the last decade. The observations are discussed in the context of molecular mechanisms and functionalities relevant to health such as neurological function, genome stability, immune response, and reproduction. Most animal and many cell studies showed increased oxidative stress caused by RF-EMF and ELF-MF. In order to estimate the risk for human health by manmade exposure, experimental studies in humans and epidemiological studies need to be considered as well.
Reduced Claudin-12 Expression Predicts Poor Prognosis in Cervical Cancer
Authors: Rahman Abidur , Kobayashi Makoto , Sugimoto Kotaro , Endo Yuta , Kojima Manabu , Furukawa Shigenori , Watanabe Takafumi , Soeda Shu , Hashimoto Yuko , Fujimori Keiya , Chiba Hideki
Published: April 2021

Background: Within the claudin (CLDN) family, CLDN12 mRNA expression is altered in various types of cancer, but its clinicopathological relevance has yet to be established due to the absence of specific antibodies (Abs) with broad applications. Methods: We generated a monoclonal Ab (mAb) against human/mouse CLDN12 and verified its specificity. By performing immunohistochemical staining and semiquantification, we evaluated the relationship between CLDN12 expression and clinicopathological parameters in tissues from 138 cases of cervical cancer. Results: Western blot and immunohistochemical analyses revealed that the established mAb selectively recognized the CLDN12 protein. Twenty six of the 138 cases (18.8%) showed low CLDN12 expression, and the disease-specific survival (DSS) and recurrence-free survival rates were significantly decreased compared with those in the high CLDN12 expression group. We also demonstrated, via univariable and multivariable analyses, that the low CLDN12 expression represents a significant prognostic factor for the DSS of cervical cancer patients (HR 3.412, p = 0.002 and HR 2.615, p = 0.029, respectively). Conclusions: It can be concluded that a reduced CLDN12 expression predicts a poor outcome for cervical cancer. The novel anti-CLDN12 mAb could be a valuable tool to evaluate the biological relevance of the CLDN12 expression in diverse cancer types and other diseases.
Cancer-Associated Adipocytes in Breast Cancer: Causes and Consequences
Authors: Rybinska Ilona , Mangano Nunzia , Tagliabue Elda , Triulzi Tiziana
Published: April 2021

Breast cancer progression is highly dependent on the heterotypic interaction between tumor cells and stromal cells of the tumor microenvironment. Cancer-associated adipocytes (CAAs) are emerging as breast cancer cell partners favoring proliferation, invasion, and metastasis. This article discussed the intersection between extracellular signals and the transcriptional cascade that regulates adipocyte differentiation in order to appreciate the molecular pathways that have been described to drive adipocyte dedifferentiation. Moreover, recent studies on the mechanisms through which CAAs affect the progression of breast cancer were reviewed, including adipokine regulation, metabolic reprogramming, extracellular matrix remodeling, and immune cell modulation. An in-depth understanding of the complex vicious cycle between CAAs and breast cancer cells is crucial for designing novel strategies for new therapeutic interventions.
Application of the Antibody-Inducing Activity of Glycosphingolipids to Human Diseases
Authors: Okuda Tetsuya
Published: April 2021

Glycosphingolipids (GSLs) are composed of a mono-, di-, or oligosaccharide and a ceramide and function as constituents of cell membranes. Various molecular species of GSLs have been identified in mammalian cells due to differences in the structures of oligosaccharides. The oligosaccharide structure can vary depending on cell lineage, differentiation stage, and pathology; this property can be used as a cell identification marker. Furthermore, GSLs are involved in various aspects of the immune response, such as cytokine production, immune signaling, migration of immune cells, and antibody production. GSLs containing certain structures exhibit strong immunogenicity in immunized animals and promote the production of anti-GSL antibodies. By exploiting this property, it is possible to generate antibodies that recognize the fine oligosaccharide structure of specific GSLs or glycoproteins. In our study using artificially synthesized GSLs (artGSLs), we found that several structural features are correlated with the antibody-inducing activity of GSLs. Based on these findings, we designed artGSLs that efficiently induce the production of antibodies accompanied by class switching and developed several antibodies that recognize not only certain glycan structures of GSLs but also those of glycoproteins. This review comprehensively introduces the immune activities of GSLs and their application as pharmaceuticals.
Polymorphisms of Dopamine Receptor Genes and Parkinson’s Disease: Clinical Relevance and Future Perspectives
Authors: Magistrelli Luca , Ferrari Marco , Furgiuele Alessia , Milner Anna Vera , Contaldi Elena , Comi Cristoforo , Cosentino Marco , Marino Franca
Published: April 2021

Parkinson’s disease (PD) is a neurodegenerative disease caused by loss of dopaminergic neurons in the midbrain. PD is clinically characterized by a variety of motor and nonmotor symptoms, and treatment relies on dopaminergic replacement. Beyond a common pathological hallmark, PD patients may present differences in both clinical progression and response to drug therapy that are partly affected by genetic factors. Despite extensive knowledge on genetic variability of dopaminergic receptors (DR), few studies have addressed their relevance as possible influencers of clinical heterogeneity in PD patients. In this review, we summarized available evidence regarding the role of genetic polymorphisms in DR as possible determinants of PD development, progression and treatment response. Moreover, we examined the role of DR in the modulation of peripheral immunity, in light of the emerging role of the peripheral immune system in PD pathophysiology. A better understanding of all these aspects represents an important step towards the development of precise and personalized disease-modifying therapies for PD.
Polypurine Reverse-Hoogsteen Hairpins as a Tool for Exon Skipping at the Genomic Level in Mammalian Cells
Authors: Noé Véronique , Ciudad Carlos J.
Published: April 2021

Therapeutic strategies for rare diseases based on exon skipping are aimed at mediating the elimination of mutated exons and restoring the reading frame of the affected protein. We explored the capability of polypurine reverse-Hoogsteen hairpins (PPRHs) to cause exon skipping in NB6 cells carrying a duplication of exon 2 of the DHFR gene that causes a frameshift abolishing DHFR activity. Methods: Different editing PPRHs were designed and transfected in NB6 cells followed by incubation in a DHFR-selective medium lacking hypoxanthine and thymidine. Surviving colonies were analyzed by DNA sequencing, RT-PCR, Western blotting and DHFR enzymatic activity. Results: Transfection of editing PPRHs originated colonies in the DHFR-selective medium. DNA sequencing results proved that the DHFR sequence in all these colonies corresponded to the wildtype sequence with just one copy of exon 2. In the edited colonies, the skipping of the additional exon was confirmed at the mRNA level, the DHFR protein was restored, and it showed high levels of DHFR activity. Conclusions: Editing-PPRHs are able to cause exon skipping at the DNA level and could be applied as a possible therapeutic tool for rare diseases.
In Vitro Activation Early Follicles: From the Basic Science to the Clinical Perspectives
Authors: Vo Kim Cat Tuyen , Kawamura Kazuhiro
Published: April 2021

Development of early follicles, especially the activation of primordial follicles, is strictly modulated by a network of signaling pathways. Recent advance in ovarian physiology has been allowed the development of several therapies to improve reproductive outcomes by manipulating early folliculogenesis. Among these, in vitro activation (IVA) has been recently developed to extend the possibility of achieving genetically related offspring for patients with premature ovarian insufficiency and ovarian dysfunction. This method was established based on basic science studies of the intraovarian signaling pathways: the phosphoinositide 3-kinase (PI3K)/Akt and the Hippo signaling pathways. These two pathways were found to play crucial roles in folliculogenesis from the primordial follicle to the early antral follicle. Following the results of rodent experiments, IVA was implemented in clinical practice. There have been multiple recorded live births and ongoing pregnancies. Further investigations are essential to confirm the efficacy and safety of IVA before used widely in clinics. This review aimed to summarize the published literature on IVA and provide future perspectives for its improvement.
Ssu72 Dual-Specific Protein Phosphatase: From Gene to Diseases
Authors: Hwang Soeun , Kim Min-Hee , Lee Chang-Woo
Published: April 2021

More than 70% of eukaryotic proteins are regulated by phosphorylation. However, the mechanism of dephosphorylation that counteracts phosphorylation is less studied. Phosphatases are classified into 104 distinct groups based on substrate-specific features and the sequence homologies in their catalytic domains. Among them, dual-specificity phosphatases (DUSPs) that dephosphorylate both phosphoserine/threonine and phosphotyrosine are important for cellular homeostasis. Ssu72 is a newly studied phosphatase with dual specificity that can dephosphorylate both phosphoserine/threonine and phosphotyrosine. It is important for cell-growth signaling, metabolism, and immune activation. Ssu72 was initially identified as a phosphatase for the Ser5 and Ser7 residues of the C-terminal domain of RNA polymerase II. It prefers the cis configuration of the serine–proline motif within its substrate and regulates Pin1, different from other phosphatases. It has recently been reported that Ssu72 can regulate sister chromatid cohesion and the separation of duplicated chromosomes during the cell cycle. Furthermore, Ssu72 appears to be involved in the regulation of T cell receptor signaling, telomere regulation, and even hepatocyte homeostasis in response to a variety of stress and damage signals. In this review, we aim to summarize various functions of the Ssu72 phosphatase, their implications in diseases, and potential therapeutic indications.
Altered microRNA Transcriptome in Cultured Human Liver Cells upon Infection with Ebola Virus
Authors: Diallo Idrissa , Ho Jeffrey , Laffont Benoit , Laugier Jonathan , Benmoussa Abderrahim , Lambert Marine , Husseini Zeinab , Soule Geoff , Kozak Robert , Kobinger Gary P. , Provost Patrick
Published: April 2021

Ebola virus (EBOV) is a virulent pathogen, notorious for inducing life-threatening hemorrhagic fever, that has been responsible for several outbreaks in Africa and remains a public health threat. Yet, its pathogenesis is still not completely understood. Although there have been numerous studies on host transcriptional response to EBOV, with an emphasis on the clinical features, the impact of EBOV infection on post-transcriptional regulatory elements, such as microRNAs (miRNAs), remains largely unexplored. MiRNAs are involved in inflammation and immunity and are believed to be important modulators of the host response to viral infection. Here, we have used small RNA sequencing (sRNA-Seq), qPCR and functional analyses to obtain the first comparative miRNA transcriptome (miRNome) of a human liver cell line (Huh7) infected with one of the following three EBOV strains: Mayinga (responsible for the first Zaire outbreak in 1976), Makona (responsible for the West Africa outbreak in 2013–2016) and the epizootic Reston (presumably innocuous to humans). Our results highlight specific miRNA-based immunity pathways and substantial differences between the strains beyond their clinical manifestation and pathogenicity. These analyses shed new light into the molecular signature of liver cells upon EBOV infection and reveal new insights into miRNA-based virus attack and host defense strategy.
Towards the Enhancement of Essential Oil Components’ Antimicrobial Activity Using New Zein Protein-Gated Mesoporous Silica Microdevices
Authors: Poyatos-Racionero Elisa , Guarí-Borràs Gemma , Ruiz-Rico María , Morellá-Aucejo Ángela , Aznar Elena , Barat José Manuel , Martínez-Máñez Ramón , Marcos María Dolores , Bernardos Andrea
Published: April 2021

The development of new food preservatives is essential to prevent foodborne outbreaks or food spoilage due to microbial growth, enzymatic activity or oxidation. Furthermore, new compounds that substitute the commonly used synthetic food preservatives are needed to stifle the rising problem of microbial resistance. In this scenario, we report herein, as far as we know, for the first time the use of the zein protein as a gating moiety and its application for the controlled release of essential oil components (EOCs). The design of microdevices consist of mesoporous silica particles loaded with essential oils components (thymol, carvacrol and cinnamaldehyde) and functionalized with the zein (prolamin) protein found in corn as a molecular gate. The zein protein grafted on the synthesized microdevices is degraded by the proteolytic action of bacterial enzymatic secretions with the consequent release of the loaded essential oil components efficiently inhibiting bacterial growth. The results allow us to conclude that the new microdevice presented here loaded with the essential oil component cinnamaldehyde improved the antimicrobial properties of the free compound by decreasing volatility and increasing local concentration.
Differential Expression of Sphingolipid Metabolizing Enzymes in Spontaneously Hypertensive Rats: A Possible Substrate for Susceptibility to Brain and Kidney Damage
Authors: Pepe Giuseppe , Cotugno Maria , Marracino Federico , Giova Susy , Capocci Luca , Forte Maurizio , Stanzione Rosita , Bianchi Franca , Marchitti Simona , Di Pardo Alba , Sciarretta Sebastiano , Rubattu Speranza , Maglione Vittorio
Published: April 2021

Alterations in the metabolism of sphingolipids, a class of biologically active molecules in cell membranes with direct effect on vascular homeostasis, are increasingly recognized as important determinant in different vascular disorders. However, it is not clear whether sphingolipids are implicated in the pathogenesis of hypertension-related cerebrovascular and renal damage. In this study, we evaluated the existence of possible abnormalities related to the sphingolipid metabolism in the brain and kidneys of two well validated spontaneously hypertensive rat strains, the stroke-prone (SHRSP) and the stroke-resistant (SHRSR) models, as compared to the normotensive Wistar Kyoto (WKY) rat strain. Our results showed a global alteration in the metabolism of sphingolipids in both cerebral and renal tissues of both hypertensive strains as compared to the normotensive rat. However, few defects, such as reduced expression of enzymes involved in the metabolism/catabolism of sphingosine-1-phosphate and in the de novo biosynthetic pathways, were exclusively detected in the SHRSP. Although further studies are necessary to fully understand the significance of these findings, they suggest that defects in specific lipid molecules and/or their related metabolic pathways may likely contribute to the pathogenesis of hypertensive target organ damage and may eventually serve as future therapeutic targets to reduce the vascular consequences of hypertension.
Hydrothermal Synthesis and Structural Investigation of a Crystalline Uranyl Borosilicate
Authors: Pace Kristen A. , Klepov Vladislav V. , Smith Mark D. , Williams Travis , Morrison Gregory , Lauterbach Jochen A. , Misture Scott T. , zur Loye Hans-Conrad
Published: April 2021

The relevance of multidimensional and porous crystalline materials to nuclear waste remediation and storage applications has motivated exploratory research focused on materials discovery of compounds, such as actinide mixed-oxoanion phases, which exhibit rich structural chemistry. The novel phase K1.8Na1.2[(UO2)BSi4O12] has been synthesized using hydrothermal methods, representing the first example of a uranyl borosilicate. The three-dimensional structure crystallizes in the orthorhombic space group Cmce with lattice parameters a = 15.5471(19) Å, b = 14.3403(17) Å, c = 11.7315(15) Å, and V = 2615.5(6) Å3, and is composed of UO6 octahedra linked by [BSi4O12]5− chains to form a [(UO2)BSi4O12]3− framework. The synthesis method, structure, results of Raman, IR, and X-ray absorption spectroscopy, and thermal stability are discussed.