bluuebunny/crossref_metadata_2025 huggingface dataset:
Neuropilin 1 (NRP1) Positively Regulates Adipogenic Differentiation in C3H10T1/2 Cells
Authors: Yu Yaqiong , Uchida-Fukuhara Yoko , Weng Yao , He Yuhan , Ikegame Mika , Wang Ziyi , Yoshida Kaya , Okamura Hirohiko , Qiu Lihong
Published: April 2023

Neuropilin 1 (NRP1), a non-tyrosine kinase receptor for several ligands, is highly expressed in many kinds of mesenchymal stem cells (MSCs), but its function is poorly understood. In this study, we explored the roles of full-length NRP1 and glycosaminoglycan (GAG)-modifiable NRP1 in adipogenesis in C3H10T1/2 cells. The expression of full-length NRP1 and GAG-modifiable NRP1 increased during adipogenic differentiation in C3H10T1/2 cells. NRP1 knockdown repressed adipogenesis while decreasing the levels of Akt and ERK1/2 phosphorylation. Moreover, the scaffold protein JIP4 was involved in adipogenesis in C3H10T1/2 cells by interacting with NRP1. Furthermore, overexpression of non-GAG-modifiable NRP1 mutant (S612A) greatly promoted adipogenic differentiation, accompanied by upregulation of the phosphorylated Akt and ERK1/2. Taken together, these results indicate that NRP1 is a key regulator that promotes adipogenesis in C3H10T1/2 cells by interacting with JIP4 and activating the Akt and ERK1/2 pathway. Non-GAG-modifiable NRP1 mutant (S612A) accelerates the process of adipogenic differentiation, suggesting that GAG glycosylation is a negative post-translational modification of NRP1 in adipogenic differentiation.
Complexes of NiII, CoII, ZnII, and CuII with Promising Anti-Tuberculosis Drug: Solid-State Structures and DFT Calculations
Authors: Ahmed Mohamed Ali , Zhernakov Maksim A. , Gilyazetdinov Edward M. , Bukharov Mikhail S. , Islamov Daut R. , Usachev Konstantin S. , Klimovitskii Alexander E. , Serov Nikita Yu. , Burilov Vladimir A. , Shtyrlin Valery G.
Published: April 2023

Four new NiII, CoII, ZnII, and CuII complexes with the promising anti-tuberculosis drug (E/Z)-N′-((5-Hydroxy-3,4-bis(hydroxymethyl)-6-methylpyridin-2-yl)methylene)-isonicotino-hydrazide (LH) were synthesized and characterized by structural methods: single-crystal X-ray diffraction, vibrational spectroscopy, and mass spectrometry. The NiII, CoII, and ZnII metal ions form only amorphous phases with various morphologies according to mass spectrometry and IR spectroscopy. The CuII forms a crystalline 1D coordination polymer with the relative formula {[CuLCl]·0.5H2O}∞1. Even though the LH ligand in the crystalline state includes a mixture of E-/Z-isomers, only the tautomeric iminol E-/Z-form is coordinated by CuII in the crystal. The copper(II) complex crystallizes in the monoclinic P21/n space group with the corresponding cell parameters a = 16.3539(11) Å, b = 12.2647(6) Å, and c = 17.4916(10) Å; α = 90°, β = 108.431(7)°, and γ = 90°. DFT calculations showed that the Z-isomer of the LH ligand in solution has the lowest formation energy due to intramolecular hydrogen bonds. According to the quantum chemical calculations, the coordination environment of the CuII atom during the transfer of the molecule into the solution remains the same as in the crystal, except for the polymeric bond, namely, distorted trigonal bipyramidal. Some of the complexes investigated can be used as effective sensors in biosystems.
Association between Cardiovascular Response and Inflammatory Cytokines in Non-Small Cell Lung Cancer Patients
Authors: Wang Xiaolin , Cao Mengying , Liu Zilong , Chen Liming , Zhou Yufei , Gao Pan , Zou Yunzeng
Published: April 2023

Cardiovascular disease is an essential comorbidity in patients with non-small cell lung cancer (NSCLC) and represents an independent risk factor for increased mortality. Therefore, careful monitoring of cardiovascular disease is crucial in the healthcare of NSCLC patients. Inflammatory factors have previously been associated with myocardial damage in NSCLC patients, but it remains unclear whether serum inflammatory factors can be utilized to assess the cardiovascular health status in NSCLC patients. A total of 118 NSCLC patients were enrolled in this cross-sectional study, and their baseline data were collected through a hospital electronic medical record system. Enzyme-linked immunosorbent assay (ELISA) was used to measure the serum levels of leukemia inhibitory factor (LIF), interleukin (IL)-18, IL-1β, transforming growth factor-β1 (TGF-β1), and connective tissue growth factor (CTGF). Statistical analysis was performed using the SPSS software. Multivariate and ordinal logistic regression models were constructed. The data revealed an increased serum level of LIF in the group using tyrosine kinase inhibitor (TKI)-targeted drugs compared to non-users (p < 0.001). Furthermore, serum TGF-β1 (area under the curve, AUC: 0.616) and cardiac troponin T (cTnT) (AUC: 0.720) levels were clinically evaluated and found to be correlated with pre-clinical cardiovascular injury in NSCLC patients. Notably, the serum levels of cTnT and TGF-β1 were found to indicate the extent of pre-clinical cardiovascular injury in NSCLC patients. In conclusion, the results suggest that serum LIF, as well as TGFβ1 together with cTnT, are potential serum biomarkers for the assessment of cardiovascular status in NSCLC patients. These findings offer novel insights into the assessment of cardiovascular health and underscore the importance of monitoring cardiovascular health in the management of NSCLC patients.
Nanocellulose-Based Thermoplastic Polyurethane Biocomposites with Shape Memory Effect
Authors: Gorbunova Marina , Grunin Leonid , Morris Robert H. , Imamutdinova Arina
Published: April 2023

In 2020, we published a review on the study of semi-crystalline thermoplastic polyurethane elastomers and composites based on the shape memory effect. The shape recovery ability of such polymers is determined by their sensitivity to temperature, moisture, and magnetic or electric fields, which in turn are dependent on the chemical properties and composition of the matrix and the nanofiller. Nanocellulose is a type of nanomaterial with high strength, high specific surface area and high surface energy. Additionally, it is nontoxic, biocompatible, environmentally friendly, and can be extracted from biomass resources. Thanks to these properties, nanocellulose can be used to enhance the mechanical properties of polymer matrices with shape memory effect and as a switching element of shape memory. This review discusses the methods for producing and properties of nanocellulose-based thermo-, moisture-, and pH-sensitive polyurethane composites. The synergistic effect of nanocellulose and carbon nanofillers and possible applications of nanocellulose-based thermoplastic polyurethane biocomposites with shape memory effect are discussed. A brief description of nanocellulose terminology is also given, along with the structure of shape memory thermoplastic polyurethanes. There is significant interest in such materials for three primary reasons: the possibility of creating a new generation of biomaterials, improving the environmental friendliness of existing materials, and exploiting the natural renewability of cellulose sources.
Experimental and Numerical Analysis of Structural Capacity of Perforated Stiffened Plates
Authors: Saad-Eldeen S. , Garbatov Yordan
Published: April 2023

This study presents experimental and numerical analyses of compressive collapse tests of initially corroded steel-stiffened plates with multiple circular openings, which may be used as tank wash plates. The ultimate load-carrying capacity and the relevant collapse modes are identified. The load-axial/lateral displacement relationships and local strain for each opening degree are analysed with developed regression equations for the ultimate capacity of stiffened plates as a function of different degrees of openings and the resting volume and residual cross-sectional area. A series of finite element analyses are performed. The experimental and numerical results are compared and show the complexity of predicting the ultimate capacity and the collapse mode as the degree of openings increases. The experimental results are compared for aged steel plates, and high-tensile stiffened plates considering the same degrees of openings, showing good agreement.
Load Frequency Control of Marine Microgrid System Integrated with Renewable Energy Sources
Authors: Zhang Guoqiang , Khan Irfan Ahmed , Daraz Amil , Basit Abdul , Khan Muhammad Irshad
Published: April 2023

In seaports, low-carbon energy systems and energy efficiency have become increasingly important as a result of the evolution of environmental and climate change challenges. In order to ensure the continued success of seaports, technological advancements must be introduced to a number of systems, such as seaport vehicles, harbor cranes, and the power sources of berthed ships. Harbor areas might need a microgrid to handle these aspects. Typically, microgrids that substitute conventional generator units with renewable energy sources (RES) suffer from system inertia problems, which adversely affect microgrid frequency stability. A load frequency controller (LFC) based on a novel modified proportional integral derivative with filter (MPIDF) is presented in this paper for enhancing the performance of marine microgrid system (MMS). The serval optimization algorithm (SOA), a recent bio-inspired optimization algorithm, is used to optimize the MPIDF controller coefficients. This controller is tested on a marine microgrid containing a number of RES such as wind turbine generators, sea wave energy, and solar generation. The efficacy of the proposed MPIDF controller is verified with respect to other controllers such as PIDF and PI. Similarly, the proposed meta-heuristic algorithm is validated as compared to other algorithms including particle swarm optimization (PSO), ant colony optimization (ACO), and jellyfish swarm optimization (JSO). This study also evaluates the robustness of the proposed controller to different perturbations in step load, changes in system parameters, and other parameter variations.
Numerical Study on Fluid Dynamic Characteristics of a Cross-Flow Fan
Authors: Cai Xiaowei , Zhang Chun , Wang Baoshou
Published: April 2023

This paper focuses on the application of a cross-flow fan (CFF) to generate propulsion for a submersible aircraft with a flying wing configuration. A numerical method is established to simulate the CFF operating both in the air and underwater. This paper then investigates the fluid dynamic characteristics of the CFF, including the velocity field, the pressure field, the cavitation distribution, the lift, and the thrust. It is concluded that proper lifts and thrusts can be obtained when the rotating speed and the angle of attack are reasonably designed. This work provides a valuable numerical methodology for studying the fluid dynamic characteristics of the CFF operating in different media and offers a technical basis for the selection of a motor system for submersible aircraft.
Novelties in Macrofungi of the Tropical Montane Cloud Forest in Mexico
Authors: Valenzuela Ricardo , Luna-Vega Isolda , Martínez-Pineda Michelle , Martínez-González César Ramiro , García-Jiménez Jesús , de la Fuente Javier , Bautista-Hernández Silvia , Acosta-Castellanos Salvador , Raymundo Tania
Published: April 2023

The tropical montane cloud forest in Mexico is the most diverse and threatened ecosystem. Mexican macrofungi numbers more than 1408 species. This study described four new species of Agaricomycetes (Bondarzewia, Gymnopilus, Serpula, Sparassis) based on molecular and morphological characteristics. Our results support that Mexico is among the most biodiverse countries in terms of macrofungi in the Neotropics.
Subtitling for the Deaf and Hard of Hearing, Audio Description and Audio Subtitling in Multilingual TV Shows
Authors: Beseghi Micòl
Published: April 2023

Multilingualism in audiovisual productions has substantially increased in recent years as a reflection of today’s globalised world. While the number of publications looking at the phenomenon from the perspective of audiovisual translation (AVT)—especially interlingual subtitling and dubbing—has grown considerably in the last decade, there seems to be relatively little research on the rendering of multilingualism from the perspective of accessibility modes, namely subtitling for the deaf and hard of hearing (SDH) and audio description (AD). This article aims to investigate how multilingualism is rendered for deaf and hard-of-hearing as well as blind and partially sighted audiences, focusing on SDH and AD, as well as audio subtitling (AST). The study analyses a small corpus of TV shows available on Netflix and aims to highlight how multilingualism is made accessible both in SDH and AD. The products selected for the study had to satisfy three main criteria: they had to be a recent production, include the presence of an L1 (English) and one or more third languages and offer both intralingual SDH (closed captions) and AD. The results show that, even within the context of a single streaming platform, the strategies applied to deal with multilingualism seem to vary quite significantly both in SDH and AD/AST, ranging from neutralisation to L3 visibility.
When Criminals Abuse the Blockchain: Establishing Personal Jurisdiction in a Decentralised Environment
Authors: Watters Casey
Published: April 2023

In August of 2022, the United States Department of Treasury sanctioned the virtual currency mixer Tornado Cash, an open-source and fully decentralised piece of software running on the Ethereum blockchain, subsequently leading to the arrest of one of its developers in the Netherlands. Not only was this the first time the Office of Foreign Assets Control (OFAC) extended its authority to sanction a foreign ‘person’ to software, but the decentralised nature of the software and global usage highlight the challenge of establishing jurisdiction over decentralised software and its global user base. The government claims jurisdiction over citizens, residents, and any assets that pass through the country’s territory. As a global financial center with most large tech companies, this often facilitates the establishment of jurisdiction over global conduct that passes through US servers. However, decentralised programs on blockchains with nodes located around the world challenge this traditional approach as either nearly all countries can claim jurisdiction over users, subjecting users to criminal laws in countries with which they have no true interaction, or they limit jurisdiction, thereby risking abuse by bad actors. This article takes a comparative approach to examine the challenges to establishing criminal jurisdiction on cryptocurrency-related crimes.
A Parameterized Leblond–Devaux Equation for Predicting Phase Evolution during Welding E36 and E36Nb Marine Steels
Authors: Fu Jun , El-Fallah G. M. A. M. , Tao Qing , Dong Hongbiao
Published: April 2023

High heat input welding can improve welding efficiency, but the impact toughness of the heat-affected zone (HAZ) deteriorates significantly. Thermal evolution in HAZ during welding is the key factor affecting welded joints’ microstructures and mechanical properties. In this study, the Leblond–Devaux equation for predicting phase evolution during the welding of marine steels was parameterized. In experiments, E36 and E36Nb samples were cooled down at different rates from 0.5 to 75 °C/s; the obtained thermal and phase evolution data were used to construct continuous cooling transformation diagrams, which were used to derive the temperature-dependent parameters in the Leblond–Devaux equation. The equation was then used to predict phase evolution during the welding of E36 and E36Nb; the quantitative experimental phase fractions of the coarse grain zone were compared with simulated results to verify the prediction results, which are in good agreement. When heat input is 100 kJ/cm, phases in the HAZ of E36Nb are primarily granular bainite, whereas for E36, the phases are mainly bainite with acicular ferrite. When heat input increases to 250 kJ/cm, ferrite and pearlite form in both steels. The predictions agree with experimental observations.
On the Aging Kinetics of a Flame-Resistant AZ91D-1.5%Ca Magnesium Alloy Processed with Ultrasonic Vibration
Authors: Gomes Inês V. , D’Errico Fabrizio , Alves José L. , Puga Hélder
Published: April 2023

The Mg-Al-Zn-Ca system has demonstrated excellent flame resistance and mechanical properties in the as-cast condition. However, the potential of these alloys to be heat-treated, e.g., by aging, as well as the influence of the initial microstructure on the precipitation kinetics, is yet to be comprehensively explored. Ultrasound treatment was applied during the solidification of an AZ91D-1.5%Ca alloy to promote microstructure refinement. Samples from treated and non-treated ingots were subjected to solution treatment at 415 °C for 480 min, followed by aging at 175 °C for up to 4920 min. The results showed that the ultrasound-treated material could reach the peak-age condition in a shorter period than the non-treated one, suggesting accelerated precipitation kinetics and, thus, enhanced aging response. However, the tensile properties showed a decrease in the peak age compared to the as-cast condition, probably due to the formation of precipitates at the grain boundaries that promote the formation of microcracks and intergranular early fracture. This research shows that tailoring the material’s as-cast microstructure may positively affect its aging response, shortening the heat treatment duration, thereby making the process less expensive and more sustainable.
Achieving Large-Capability Adsorption of Hg0 in Wet Scrubbing by Defect-Rich Colloidal Copper Sulfides under High-SO2 Atmosphere
Authors: Xie Xiaofeng , Chen Hao , Liu Xudong , Xiang Kaisong , Liu Hui
Published: April 2023

This paper reports on a novel method to remove Hg0 in the wet scrubbing process using defect-rich colloidal copper sulfides for reducing mercury emissions from non-ferrous smelting flue gas. Unexpectedly, it migrated the negative effect of SO2 on mercury removal performance, while also enhancing Hg0 adsorption. Colloidal copper sulfides demonstrated the superior Hg0 adsorption rate of 306.9 μg·g−1·min−1 under 6% SO2 + 6% O2 atmosphere with a removal efficiency of 99.1%, and the highest-ever Hg0 adsorption capacity of 736.5 mg·g−1, which was 277% higher than all other reported metal sulfides. The Cu and S sites transformation results reveal that SO2 could transform the tri-coordinate S sites into S22− on copper sulfides surfaces, while O2 regenerated Cu2+ via the oxidation of Cu+. The S22− and Cu2+ sites enhanced Hg0 oxidation, and the Hg2+ could strongly bind with tri-coordinate S sites. This study provides an effective strategy to achieve large-capability adsorption of Hg0 from non-ferrous smelting flue gas.
Hydrogen Absorption Performance and O2 Poisoning Resistance of Pd/ZrCo Composite Film
Authors: Qian Yiyao , Qian Ruijun , Feng Hetian , Zhu Dachuan , Wu Chaoling
Published: April 2023

In order to enhance the hydrogen absorption performance and poisoning resistance of ZrCo to O2, Pd/ZrCo composite films were prepared by direct current magnetron sputtering. The results show that the initial hydrogen absorption rate of the Pd/ZrCo composite film increased significantly due to the catalytic effect of Pd compared with the ZrCo film. In addition, the hydrogen absorption properties of Pd/ZrCo and ZrCo were tested in poisoned hydrogen mixed with 1000 ppm O2 at 10–300 °C, where the Pd/ZrCo films maintained a better resistance to O2 poisoning below 100 °C. The mechanism of poisoning was investigated jointly by first-principles calculation combined with SEM-EDS elemental mapping tests. It is shown that the poisoned Pd layer maintained the ability to promote the decomposition of H2 into hydrogen atoms and their rapid transfer to ZrCo.
New Partially Water-Soluble Feedstocks for Additive Manufacturing of Ti6Al4V Parts by Material Extrusion
Authors: Eickhoff Ralf , Antusch Steffen , Nötzel Dorit , Hanemann Thomas
Published: April 2023

In this work, a process chain for the realization of dense Ti6Al4V parts via different material extrusion methods will be introduced applying eco-friendly partially water-soluble binder systems. In continuation of earlier research, polyethylene glycol (PEG) as a low molecular weight binder component was combined either with poly(vinylbutyral) (PVB) or with poly(methylmethacrylat) (PMMA) as a high molecular weight polymer and investigated with respect to their usability in FFF and FFD. The additional investigation of different surfactants’ impact on the rheological behaviour applying shear and oscillation rheology allowed for a final solid Ti6Al4V content of 60 vol%, which is sufficient to achieve after printing, debinding and thermal densification parts with densities better than 99% of the theoretical value. The requirements for usage in medical applications according to ASTM F2885-17 can be fulfilled depending on the processing conditions.
Intelligent Fault Diagnosis of an Aircraft Fuel System Using Machine Learning—A Literature Review
Authors: Li Jiajin , King Steve , Jennions Ian
Published: April 2023

The fuel system, which aims to provide sufficient fuel to the engine to maintain thrust and power, is one of the most critical systems in the aircraft. However, possible degradation modes, such as leakage and blockage, can lead to component failure, affect performance, and even cause serious accidents. As an advanced maintenance strategy, Condition Based Maintenance (CBM) can provide effective coverage, by combining state-of-the-art sensors with data acquisition and analysis techniques to guide maintenance before the asset’s degradation becomes serious. Artificial Intelligence (AI), particularly machine learning (ML), has proved effective in supporting CBM, for analyzing data and generating predictions regarding the asset’s health condition, thus influencing maintenance plans. However, from an engineering perspective, the output of ML algorithms, usually in the form of data-driven neural networks, has come into question in practice, as it can be non-intuitive and lacks the ability to provide unambiguous engineering signals to maintainers, making it difficult to trust. Engineers are interested in a deterministic decision-making process and how it is being revealed; algorithms should be able to certify and convince engineers to approve recommended actions. Explainable AI (XAI) has emerged as a potential solution, providing some of the logic on how the output is derived from the input given, which may help users understand the diagnostic result of the algorithm. In order to inspire and advise data scientists and engineers who are about to develop and use AI approaches in fuel systems, this paper explores the literature of experiment, simulation, and AI-based diagnostics for the fuel system to make an informed statement as to the progress that has been made in intelligent fault diagnostics for fuel systems, emphasizing the necessity of giving unambiguous engineering signals to maintainers, as well as highlighting potential areas for future research.
Structural, Elastic, Electronic, and Magnetic Properties of Full-Heusler Alloys Sc2TiAl and Sc2TiSi Using the FP-LAPW Method
Authors: Al-Masri Khadejah M. , Abu-Jafar Mohammed S. , Farout Mahmoud , Dahliah Diana , Mousa Ahmad A. , Azar Said M. , Khenata Rabah
Published: April 2023

In this article, the structural, elastic, electronic, and magnetic characteristics of both regular and inverse Heusler alloys, Sc2TiAl and Sc2TiSi, were investigated using a full-potential, linearized augmented plane-wave (FP-LAPW) method, within the density functional theory. The optimized structural parameters were determined from the minimization of the total energy versus the volume of the unit cell. The band structure and DOS calculations were performed within the generalized gradient approximation (GGA) and modified Becke–Johnson approaches (mBJ-GGA), employed in the Wien2K code. The density of states (DOS) and band structure (BS) indicate the metallic nature of the regular structure of the two compounds. The total spin magnetic moments for the two compounds were consistent with the previous theoretical results. We calculated the elastic properties: bulk moduli, B, Poisson’s ratio, ν, shear modulus, S, Young’s modulus (Y), and the B/s ratio. Additionally, we used Blackman’s diagram and Every’s diagram to compare the elastic properties of the studied compounds, whereas Pugh’s and Poisson’s ratios were used in the analysis of the relationship between interatomic bonding type and physical properties. Mechanically, we found that the regular and inverse full-Heusler compounds Sc2TiAl and Sc2TiSi were stable. The results agree with previous studies, providing a road map for possible uses in electronic devices.
Heuristics for Quantum Computing Dealing with 3-SAT
Authors: Paulet Jose J. , LLana Luis F. , Calvo Hernán Indíbil , Mezzini Mauro , Cuartero Fernando , Pelayo Fernando L.
Published: April 2023

The SAT problem is maybe one of the most famous NP-complete problems. This paper deals with the 3-SAT problem. We follow a sort of incremental strategy to save computational costs with respect to the classical quantum computing approach. We present an heuristics that leads this strategy, improving the performance of the purely random incremental scheme. We finally validate our approach by means of a thorough empirical study.
High Steep Rock Slope Instability Mechanism Induced by the Pillar Deterioration in the Mountain Mining Area
Authors: Chen Lu , Yu Xiangxi , Luo Ron , Zeng Ling , Cao Hongtao
Published: April 2023

In hilly regions, landslides or slope failures are very common phenomena, when underground mineral resources are excavated. In this study, some landslide disasters in a mountain mining area were analyzed. The engineering geological and instability reason were investigated. The numerical simulation of a high steep rock slope disturbed by a room and pillar mine was established. The failure process of a high steep rock slope induced by the pillar deterioration was analyzed to reveal the characteristics of deformation and sliding. The results show that the pillar plays an important role in maintaining the stability of the slope, if the pillar can support the overlying rock mass, only a tiny deformation will be induced. When the pillar fails and the roof caves, the overlying rock mass above the room and pillar goaf will rapidly subside, and the crack evolution of slope is induced, forming the potential slip surface. The landslide mass gradually moves. When the rock mass at the middle and lower of the slope is squeezed out, slope sliding will be induced. The failure process can be divided into four stages as follow: tiny displacement is caused by the mining, roof collapse is caused by the pillar failure, the potential slip surface is formed from the crack evolution; the slope sliding is induced by the fracturing of rock mass at the middle and lower of the slope.
Graph-Regularized, Sparsity-Constrained Non-Negative Matrix Factorization with Earth Mover’s Distance Metric
Authors: Li Shunli , Lu Linzhang , Liu Qilong , Chen Zhen
Published: April 2023

Non-negative matrix factorization (NMF) is widely used as a powerful matrix factorization tool in data representation. However, the traditional NMF, measured by Euclidean distance or Kullback–Leibler distance, does not take into account the internal implied geometric information of the dataset and cannot measure the distance between samples as well as possible. To remedy the defects, in this paper, we propose the NMF method with Earth mover’s distance as a metric, for short GSNMF-EMD. It combines graph regularization and L1/2 smooth constraints. The GSNMF-EMD method takes into account the intrinsic implied geometric information of the dataset and can produce more sparse and stable local solutions. Experiments on two specific image datasets showed that the proposed method outperforms related state-of-the-art methods.
Synthesis and Antibiotic Activity of Chitosan-Based Comb-like Co-Polypeptides
Authors: Enright Timothy P. , Garcia Dominic L. , Storti Gia , Heindl Jason E. , Sidorenko Alexander
Published: April 2023

Infections caused by multidrug-resistant Gram-negative bacteria have been named one of the most urgent global health threats due to antimicrobial resistance. Considerable efforts have been made to develop new antibiotic drugs and investigate the mechanism of resistance. Recently, Anti-Microbial Peptides (AMPs) have served as a paradigm in the design of novel drugs that are active against multidrug-resistant organisms. AMPs are rapid-acting, potent, possess an unusually broad spectrum of activity, and have shown efficacy as topical agents. Unlike traditional therapeutics that interfere with essential bacterial enzymes, AMPs interact with microbial membranes through electrostatic interactions and physically damage cell integrity. However, naturally occurring AMPs have limited selectivity and modest efficacy. Therefore, recent efforts have focused on the development of synthetic AMP analogs with optimal pharmacodynamics and an ideal selectivity profile. Hence, this work explores the development of novel antimicrobial agents which mimic the structure of graft copolymers and mirror the mode of action of AMPs. A family of polymers comprised of chitosan backbone and AMP side chains were synthesized via the ring-opening polymerization of the N-carboxyanhydride of l-lysine and l-leucine. The polymerization was initiated from the functional groups of chitosan. The derivatives with random- and block-copolymer side chains were explored as drug targets. These graft copolymer systems exhibited activity against clinically significant pathogens and disrupted biofilm formation. Our studies highlight the potential of chitosan-graft-polypeptide structures in biomedical applications.
Marine Natural Products as Anticancer Agents 2.0
Authors: Alves Celso , Diederich Marc
Published: April 2023

Global cancer incidence and death are expected to increase to 28 [...]
Cytoreductive Nephrectomy and Metastatic Renal Cell Carcinoma: State of the Art and Future Perspectives
Authors: Napolitano Luigi , Manfredi Celeste , Cirillo Luigi , Fusco Giovanni Maria , Passaro Francesco , Abate Marco , La Rocca Roberto , Mastrangelo Francesco , Spirito Lorenzo , Pandolfo Savio Domenico , Crocetto Felice , Arcaniolo Davide , Barone Biagio
Published: April 2023

In the past decades, several treatments have been proposed for the management of metastatic renal cell carcinoma (mRCC). Among these, cytoreductive nephrectomy (CN) represents a controversial and open issue in the era of targeted therapy and novel immunotherapy with immune checkpoint inhibitors. Two important studies, CARMENA and SURTIME, analyzed therapy with sunitinib with or without CN, and immediate CN followed by sunitinib versus a deferred CN after three cycles of sunitinib, respectively. CARMENA showed the non-inferiority of sunitinib alone versus sunitinib plus CN, whereas SURTIME showed no difference in progression-free survival (PFS), but a better median OS among patients with deferred CN. Therefore, more prospective clinical trials and appropriate patient identification are necessary to support CN in this new scenario. This review provides a snapshot of the current evidence for CN in mRCC, discusses the management strategies, and offers perspectives on the direction of future research.
Diagnostic Test Accuracy of Artificial Intelligence in Detecting Periapical Periodontitis on Two-Dimensional Radiographs: A Retrospective Study and Literature Review
Authors: Issa Julien , Jaber Mouna , Rifai Ismail , Mozdziak Paul , Kempisty Bartosz , Dyszkiewicz-Konwińska Marta
Published: April 2023

This study aims to evaluate the diagnostic accuracy of artificial intelligence in detecting apical pathosis on periapical radiographs. A total of twenty anonymized periapical radiographs were retrieved from the database of Poznan University of Medical Sciences. These radiographs displayed a sequence of 60 visible teeth. The evaluation of the radiographs was conducted using two methods (manual and automatic), and the results obtained from each technique were afterward compared. For the ground-truth method, one oral and maxillofacial radiology expert with more than ten years of experience and one trainee in oral and maxillofacial radiology evaluated the radiographs by classifying teeth as healthy and unhealthy. A tooth was considered unhealthy when periapical periodontitis related to this tooth had been detected on the radiograph. At the same time, a tooth was classified as healthy when no periapical radiolucency was detected on the periapical radiographs. Then, the same radiographs were evaluated by artificial intelligence, Diagnocat (Diagnocat Ltd., San Francisco, CA, USA). Diagnocat (Diagnocat Ltd., San Francisco, CA, USA) correctly identified periapical lesions on periapical radiographs with a sensitivity of 92.30% and identified healthy teeth with a specificity of 97.87%. The recorded accuracy and F1 score were 96.66% and 0.92, respectively. The artificial intelligence algorithm misdiagnosed one unhealthy tooth (false negative) and over-diagnosed one healthy tooth (false positive) compared to the ground-truth results. Diagnocat (Diagnocat Ltd., San Francisco, CA, USA) showed an optimum accuracy for detecting periapical periodontitis on periapical radiographs. However, more research is needed to assess the diagnostic accuracy of artificial intelligence-based algorithms in dentistry.
Effect of Vegetable Juices on Properties of Two Resin Composites Used for Dental Caries Management
Authors: Tighiceanu Claudia , Bulai Elena Raluca , Iatcu Oana Camelia , Dulucheanu Constantin , Nemtoi Alexandru
Published: April 2023

Background and Objectives: Resin composites represent a class of materials widely used in restorative dentistry due to patient demands for better aesthetics. Colour changes in composite resins can occur due to intrinsic and extrinsic factors. Beverages, such as vegetable juices, can be some of these extrinsic factors. The purpose of this study was to investigate the colour stability and modification of microhardness of two resin composites before and after immersion in different vegetable juices. Materials and Methods: The colour of two resin composite materials (Gradia Direct Anterior-shade A2 and Valux Plus—shade A2) was measured before and after immersion in four different solutions: distilled water (as control), beetroot, carrot, and tomato juice. Colour values (L *, a *, b *) were measured over a white background with a colorimeter, using the CIE L *a *b * system. Colour change values were calculated after 1, 3, 5, and 7 days of immersion. Microhardness measurements were taken before and after seven days immersion in test media. Repeated measures analysis of variance (ANOVA) and independent t-tests were applied for statistical analysis. Results: All vegetable juices produced statistically different discolouration after immersion for 7 days (p < 0.05). Tomato juice produced the most discolouration for the Gradia Direct specimens, whereas beetroot juice and carrot juice produced the most discolouration for the Valux Plus specimens. Microhardness of the materials immersed for 7 days in vegetable juices was reduced compared to the ones immersed in distilled water. Conclusions: Vegetable juices, immersion time, and dental resin composites are significant factors that may affect colour stability and microhardness of composite resins.
Quality of Life in Cervical Cancer Survivors Treated with Concurrent Chemoradiotherapy
Authors: Stuopelytė Raminta , Žukienė Guoda , Breivienė Rūta , Rudaitis Vilius , Bartkevičienė Daiva
Published: April 2023

Background and Objectives: Cervical cancer is the fourth most common cancer in women globally. As survival rates gradually increase, it becomes necessary to assess the quality of life (QoL) after treatment. It is known that different treatment modalities have different effects on QoL. Therefore, we aimed to evaluate the QoL of cervical cancer survivors (CCSs) treated with concurrent chemoradiotherapy (CCRT). Materials and Methods: A cross-sectional monocentric study, conducted in Vilnius university hospital Santaros klinikos between November 2018 and November 2022, included 20 women, who were interviewed once using the European Organization for Research and Treatment of Cancer (EORTC)-designed Quality-of-Life questionnaire cervical cancer module (QLQ-CX24). The sociodemographic and clinical data as well as the results of the questionnaire are presented in mean, standard deviation and percentages. The QoL scores were compared between different age and stage groups using the Mann–Whitney U test. Results: Twenty participants, aged from 27 to 55 years, with a mean age of 44 years (SD = 7.6) participated in the study. All the participants were CCSs with an International Federation of Gynecology and Obstetrics (FIGO) stage from IB to IIIB and all of them were treated with CCRT. The symptom experience was relatively low and revealed a good result (21.8, SD = 10.2). Mean scores on body image, sexual/vaginal functioning, menopausal symptoms and sexual worry scales indicated moderate functioning and a moderate level of some of the cervical cancer specific symptoms after CCRT. Sexual activity and sexual enjoyment of the CCSs were low (11.7 (SD = 16.3), 14.3 (SD = 17.8), respectively). Conclusions: Cervical cancer survivors report a relatively good quality of life regarding symptom experience; however, women following concurrent chemoradiotherapy tend not to be sexually active and rarely feel sexual enjoyment. In addition, this treatment modality negatively affects a woman’s body image and self-perception as a woman.
Five Level Triage vs. Four Level Triage in a Quaternary Emergency Department: National Analysis on Waiting Time, Validity, and Crowding—The CREONTE (Crowding and RE-Organization National TriagE) Study Group
Authors: Savioli Gabriele , Ceresa Iride Francesca , Bressan Maria Antonietta , Piccini Gaia Bavestrello , Varesi Angelica , Novelli Viola , Muzzi Alba , Cutti Sara , Ricevuti Giovanni , Esposito Ciro , Voza Antonio , Desai Antonio , Longhitano Yaroslava , Saviano Angela , Piccioni Andrea , Piccolella Fabio , Bellou Abdel , Zanza Christian , Oddone Enrico
Published: April 2023

Background and Objectives: Triage systems help provide the right care at the right time for patients presenting to emergency departments (EDs). Triage systems are generally used to subdivide patients into three to five categories according to the system used, and their performance must be carefully monitored to ensure the best care for patients. Materials and Methods: We examined ED accesses in the context of 4-level (4LT) and 5-level triage systems (5LT), implemented from 1 January 2014 to 31 December 2020. This study assessed the effects of a 5LT on wait times and under-triage (UT) and over-triage (OT). We also examined how 5LT and 4LT systems reflected actual patient acuity by correlating triage codes with severity codes at discharge. Other outcomes included the impact of crowding indices and 5LT system function during the COVID-19 pandemic in the study populations. Results: We evaluated 423,257 ED presentations. Visits to the ED by more fragile and seriously ill individuals increased, with a progressive increase in crowding. The length of stay (LOS), exit block, boarding, and processing times increased, reflecting a net raise in throughput and output factors, with a consequent lengthening of wait times. The decreased UT trend was observed after implementing the 5LT system. Conversely, a slight rise in OT was reported, although this did not affect the medium-high-intensity care area. Conclusions: Introducing a 5LT improved ED performance and patient care.
Influence of Pump Current Waveform on The Mitigation of Transverse Mode Instability in Fiber Laser Oscillator
Authors: Chai Junyu , Liu Wenguang , Wang Xiaolin , Zhou Qiong , Zhang Jiangbin , Zhang Hanwei , Liu Pengfei , Lu Yao , Zhang Dan , Jiang Zongfu , Zhao Guomin
Published: April 2023

We carry out a detailed investigation of TMI mitigation by pump modulation based on multiple current waveforms in a fiber laser oscillator. Compared with continuous wave (CW), the modulation of various waveforms, including sinusoidal wave, triangular wave, and pulse wave with a duty cycle of 50% and 60%, can increase the TMI threshold. The average output power of a stabilized beam is boosted via the adjustment of phase difference between the signal channels. The TMI threshold is increased to 270 W under a modulation of pulse wave (duty cycle: 60%) with a phase difference of 440 μs, where the beam quality is 1.45. This threshold can be further improved by adding groups of pump LDs and drivers, which is a promising approach for beam stabilization of high-power fiber lasers.
A Brief Review on Cerium Oxide (CeO2NPs)-Based Scaffolds: Recent Advances in Wound Healing Applications
Authors: Allu Ishita , Kumar Sahi Ajay , Kumari Pooja , Sakhile Karunya , Sionkowska Alina , Gundu Shravanya
Published: April 2023

The process of wound healing is complex and involves the interaction of multiple cells, each with a distinct role in the inflammatory, proliferative, and remodeling phases. Chronic, nonhealing wounds may result from reduced fibroblast proliferation, angiogenesis, and cellular immunity, often associated with diabetes, hypertension, vascular deficits, immunological inadequacies, and chronic renal disease. Various strategies and methodologies have been explored to develop nanomaterials for wound-healing treatment. Several nanoparticles such as gold, silver, cerium oxide and zinc possess antibacterial properties, stability, and a high surface area that promotes efficient wound healing. In this review article, we investigate the effectiveness of cerium oxide nanoparticles (CeO2NPs) in wound healing—particularly the effects of reducing inflammation, enhancing hemostasis and proliferation, and scavenging reactive oxygen species. The mechanism enables CeO2NPs to reduce inflammation, modulate the immunological system, and promote angiogenesis and tissue regeneration. In addition, we investigate the efficacy of cerium oxide-based scaffolds in various wound-healing applications for creating a favorable wound-healing environment. Cerium oxide nanoparticles (CeO2NPs) exhibit antioxidant, anti-inflammatory, and regenerative characteristics, enabling them to be ideal wound healing material. Investigations have shown that CeO2NPs can stimulate wound closure, tissue regeneration, and scar reduction. CeO2NPs may also reduce bacterial infections and boost wound-site immunity. However, additional study is needed to determine the safety and efficacy of CeO2NPs in wound healing and their long-term impacts on human health and the environment. The review reveals that CeO2NPs have promising wound-healing properties, but further study is needed to understand their mechanisms of action and ensure their safety and efficacy.
Whole-Genome Sequence of Lactococcus lactis Subsp. lactis LL16 Confirms Safety, Probiotic Potential, and Reveals Functional Traits
Authors: Mileriene Justina , Aksomaitiene Jurgita , Kondrotiene Kristina , Asledottir Tora , Vegarud Gerd Elisabeth , Serniene Loreta , Malakauskas Mindaugas
Published: April 2023

Safety is the most important criteria of any substance or microorganism applied in the food industry. The whole-genome sequencing (WGS) of an indigenous dairy isolate LL16 confirmed it to be Lactococcus lactis subsp. lactis with genome size 2,589,406 bp, 35.4% GC content, 246 subsystems, and 1 plasmid (repUS4). The Nextera XT library preparation kit was used to generate the DNA libraries, and the sequencing was carried out on an Illumina MiSeq platform. In silico analysis of L. lactis LL16 strain revealed non-pathogenicity and the absence of genes involved in transferable antimicrobial resistances, virulence, and formation of biogenic amines. One region in the L. lactis LL16 genome was identified as type III polyketide synthases (T3PKS) to produce putative bacteriocins lactococcin B, and enterolysin A. The probiotic and functional potential of L. lactis LL16 was investigated by the presence of genes involved in adhesion and colonization of the host’s intestines and tolerance to acid and bile, production of enzymes, amino acids, and B-group vitamins. Genes encoding the production of neurotransmitters serotonin and gamma-aminobutyric acid (GABA) were detected; however, L. lactis LL16 was able to produce only GABA during milk fermentation. These findings demonstrate a variety of positive features that support the use of L. lactis LL16 in the dairy sector as a functional strain with probiotic and GABA-producing properties.
n-3 PUFA Promotes Ferroptosis in PCOS GCs by Inhibiting YAP1 through Activation of the Hippo Pathway
Authors: Zhang Peiwen , Pan Yuheng , Wu Shuang , He Yuxu , Wang Jinyong , Chen Lei , Zhang Shunhua , Zhang Hui , Zhao Ye , Niu Lili , Gan Mailin , Wang Yan , Shen Linyuan , Zhu Li
Published: April 2023

Polycystic ovary syndrome (PCOS) is an endocrine disorder characterized by hyperandrogenemia with multiple suspended sinus follicles, thickened cortical tissue, and excessive proliferation of ovarian granulosa cells that severely affects the fertility and quality of life of women. The addition of n-3 PUFA to the diet may slightly reduce body weight and greatly alleviate disturbed blood hormone levels in PCOS mice. We treated KGN as a cell model for n-3 PUFA addition and showed that n-3 PUFA inhibited the proliferation of GCs and promoted ferroptosis in ovarian granulosa cells. We used CCK-8, fluorescence quantitative transmission electron microscopy experiments and ferroptosis marker gene detection and other methods. Furthermore, n-3 PUFA was found to promote YAP1 exocytosis by activating Hippo and weakening the cross-talk between YAP1 and Nrf2 by activating the Hippo signaling pathway. In this study, we found that n-3 PUFA inhibited the over proliferation of granulosa cells in ovarian follicles by activating Hippo, promoting YAP1 exocytosis, weakening the cross-talk between YAP1 and Nrf2, and ultimately activating the ferroptosis sensitivity of ovarian granulosa cells. We demonstrate that n-3 PUFA can alleviate the hormonal and estrous cycle disorder with PCOS by inhibiting the YAP1-Nrf2 crosstalk that suppresses over proliferating ovarian granulosa cells and promotes iron death in GCs. These findings reveal the molecular mechanisms by which n-3 PUFA attenuates PCOS and identify YAP1-Nrf2 as a potential therapeutic target for regulation granulosa cells in PCOS.
Exploring the Benefits of Phycocyanin: From Spirulina Cultivation to Its Widespread Applications
Authors: Fernandes Raquel , Campos Joana , Serra Mónica , Fidalgo Javier , Almeida Hugo , Casas Ana , Toubarro Duarte , Barros Ana I. R. N. A.
Published: April 2023

Large-scale production of microalgae and their bioactive compounds has steadily increased in response to global demand for natural compounds. Spirulina, in particular, has been used due to its high nutritional value, especially its high protein content. Promising biological functions have been associated with Spirulina extracts, mainly related to its high value added blue pigment, phycocyanin. Phycocyanin is used in several industries such as food, cosmetics, and pharmaceuticals, which increases its market value. Due to the worldwide interest and the need to replace synthetic compounds with natural ones, efforts have been made to optimize large-scale production processes and maintain phycocyanin stability, which is a highly unstable protein. The aim of this review is to update the scientific knowledge on phycocyanin applications and to describe the reported production, extraction, and purification methods, including the main physical and chemical parameters that may affect the purity, recovery, and stability of phycocyanin. By implementing different techniques such as complete cell disruption, extraction at temperatures below 45 °C and a pH of 5.5–6.0, purification through ammonium sulfate, and filtration and chromatography, both the purity and stability of phycocyanin have been significantly improved. Moreover, the use of saccharides, crosslinkers, or natural polymers as preservatives has contributed to the increased market value of phycocyanin.