bluuebunny/crossref_metadata_2025 huggingface dataset:
Forest Biometric Systems in Mexico: A Systematic Review of Available Models
Authors: López-Martínez Jorge Omar , Vargas-Larreta Benedicto , González Edgar J. , Corral-Rivas José Javier , Aguirre-Calderón Oscar A. , Treviño-Garza Eduardo J. , De los Santos-Posadas Héctor M. , Martínez-Salvador Martin , Zamudio-Sánchez Francisco J. , Aguirre-Calderón Cristóbal Gerardo
Published: April 2022

Biometric systems are the basis of forest management and consist of a set of equations that describe the relationships between forest attributes and dendrometric variables. A systematic review of the state of the art of biometric systems in Mexico was carried out by a Mexican consortium (10 researchers), covering a period of 50 years ca (1970–2019), using the main scientific literature delivered by a systematic search (WoS, Scopus, Scielo, Redalyc) and a targeted search (theses, technical reports, etc.). A single selection criterion was established for the inclusion of information in the analysis: the document had to present at least one of the equations of interest. We found 376 documents containing 2524 equations for volume (69%), diameter (11%), height (9%) and site index (11%). These equations were developed for forest species mainly from temperate regions (88%), such as pine (66%) and oak (9%). Consequently, the Mexican states with the highest number of equations were Durango (28%), Chihuahua (17%), Hidalgo (13%) and Oaxaca (8%). Although large, the number of equations identified concentrated on a relatively small number of models: Schumacher & Hall and Fang et al. for volume; Chapman-Richards and Schumacher for site index and diameter; and Chapman-Richards and the allometric equation for height. An analysis of model fit, measured through R2, showed that, on average, the volume, diameter and site index models show high fit (R2 = 0.96), although this pattern was more consistent in the volume models. Publication bias was evaluated by means of a funnel plot analysis, with no apparent bias identified. A limitation of our study is that the information obtained is not updated to the present year; however, the 50-year trends allow us to assume that no recent significant changes in the patterns exist. Finally, we highlight the need to assess the predictive ability of the models to ensure accurate estimates to support better forest management decisions.
Exploring the Branch Wood Supply Potential of an Agroforestry System with Strategically Designed Harvesting Interventions Based on Terrestrial LiDAR Data
Authors: Bohn Reckziegel Rafael , Mbongo Werner , Kunneke Anton , Morhart Christopher , Sheppard Jonathan P. , Chirwa Paxie , du Toit Ben , Kahle Hans-Peter
Published: April 2022

Agroforestry systems hold potential for wood and tree biomass production without the need of felling trees. Branch wood harvesting provides access to considerable amounts of lignocellulosic biomass while leaving the tree standing. Aiming at alternatives for wood provision, we assessed the actual woody structure of a silvopastoral system in the African Savannah ecoregion, utilising terrestrial LiDAR technology and quantitative structure models to simulate branch removals and estimate harvesting yields. In addition, the stand structure and harvested wood were examined for the provision of four types of assortments meeting local needs, and operational metrics for each treatment were derived. The stand had large variability in woody structures. Branch harvesting interventions removed up to 18.2% of total stand volume, yielded 5.9 m3 ha−1 of branch wood, and delivered 2.54 m3 ha−1 of pole wood quality, retaining on average more than 75% of the original tree structures. Among the most intense simulations, a mean of 54.7 litres (L) of branch wood was provided per tree, or approximately 34.2 kg of fresh biomass. The choice of an ideal harvesting treatment is subject to practitioners’ interests, while the discussion on aspects of the operation, and stand and tree conditions after treatment, together with outputs, assist decision making. The partitioning of tree structures and branch removal simulations are tools to support the design of tending operations aiming for wood and tree biomass harvesting in agroforestry systems while retaining different functional roles of trees in situ.
Intraspecific Pollen Morphology Variation and Its Responses to Environmental Factors of Wild Cathaya argyrophylla Chun Et Kuang Endemic to China
Authors: Xiao Fen , She Yuchen , She Jiyun , Wang Yun , Wu Fei , Xie Peng , Chen Qianxin
Published: April 2022

Studying the pollen morphology of this remnant and endemic wild species of Cathaya argyrophylla can be of use for paleobiologists. During this study, 23 genotypes sampled from four natural populations in two regions of Hunan Province, China. A total of 460 pollen grains were analyzed for seven quantitative and seven qualitative traits (including five new traits). Three quantitative traits (B, P, and A) (Width of the saccus (B); Length of the polar axis (P); Length of the saccus (A)) and four qualitative traits (O-CO, B-SD, O-CSR, and B-SU) (Pollen corpus outlined in the polar distal view (O-CO); Whether the outline of two sacci was distinct or not in the polar proximal view (B-SD); Roughness degree between corpus from the polar proximal view and the sacci from the polar distal view (O-CSR); Whether the overall size of two sacci was uniform or not (B-SU)) were the diagnostic pollen features that could possible to differentiate one population and classified 23 samples into two, three, or four clusters. Furthermore, 24 environmental factors were evaluated and precipitation factors effected more on pollen morphology than geographic and temperature factors, which including annual precipitation (bio12), precipitation of wettest month and driest month (bio13, bio14), precipitation seasonality (bio15) and monthly averaged precipitation in May (05-precip). The main precipitation and temperature factors exhibited positive and negative correlation with pollen size (B and E (Equatorial diameter (E))), respectively. This article provides deeper insight into intraspecific variability of pollen grains of C. argyrophylla, which have been investigated for the first time. In addition, the insights gained from this study could assist with the seed breeding and population reproduction of the endangered C. argyrophylla tree.
The Influence of Harbin Forest–River Ecological Corridor Construction on the Restoration of Mollisols in Cold Regions of China
Authors: Xu Huibo , Wu Songtao , Diehl Jessica Ann
Published: April 2022

Artificial ecological corridors (AECs) are internationally approved ecological restoration and climate mitigation strategies. The width and recovery time indices of AECs directly affect the restoration efficiency of degraded soil nutrients. However, there is a lack of comprehensive and quantitative evaluation research on the construction factors of AECs from the perspective of soil fertility improvement. This research aimed to examine the critical ecological corridor construction factors affecting Mollisols’ eco-chemometrics and give a scientific scope. We collected 55 Mollisol samples at different restoration years (0–35 years) and different distances (0–280 m) from the AEC of the Ashi River, a typical Mollisol restoration area in Harbin, and the cold regions of China. We measured the distances, restoration years, soil thickness, pH, electrical conductivity (EC), cation exchange capacity (CEC), soil total organic carbon (SOC), soil total organic matter (SOM), dry matter content (DMC), and the proportion of nitrogen (TN), phosphorus (TP) and potassium (TK). The results are as follows: (1) Within the AEC, there were significant differences in soil stoichiometric characteristics in different restoration years and locations; after restoration for 10–35 years, the soil stoichiometric characteristics reach or exceed the reference value of Mollisols. (2) It is feasible to restore large-scale degraded Mollisols through ecological corridors. In this recovery process, the soil nutrients first decreased, then increased, and finally reached and exceeded the reference value of normal Mollisols. (3) Soil nutrient accumulation was related to ecological corridor width and recovery time. The recommended unilateral width of the ecological corridor based on Mollisols’ CEC and SOC indices for restoration is 175–225 m, and the restoration period is 22.7–35 years based on Mollisols’ EC and SOC indices for restoration. This study demonstrated the change mechanism of Mollisols in AECs based on recovery time and location, and provided the basis for the Chinese government to formulate policies for Mollisol remediation.
Seasonal Eco-Physiology Characteristics of Four Evergreen Rhododendron Species to the Subalpine Habitats
Authors: Li Huie , Guo Qiqiang , Yang Lan , Quan Hong , Wang Shuli
Published: April 2022

Four evergreen broadleaf Rhododendron spp. (Rhododendrons), namely, Rhododendron aganniphum, R. nyingchiense, R. wardii, and R. triflorum, occur in harsh subalpine habitats in the southwest Qinghai-Tibet Plateau (QTP), China. Considering that the four Rhododendrons cannot escape their unique environment, they must evolve a set of adaptations to survive, but the information is lacking. To uncover their physiological adaptation characteristics, in the present study, we monitored their physiological characteristics by determination of their seasonal variation in antioxidant enzyme activity, osmotic adjustment substrates, and carbohydrate contents, and their pigment content and photosynthetic efficiency. The results showed that superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) activities and proline content of four Rhododendrons had a significant difference in autumn and were insignificant in summer. Specifically, R. aganniphum had the maximum protective enzyme activity and proline content in winter as well as chl a, b, and car contents. The values of maximal quantum yield (Fv/Fm), photochemical efficiency (ΦPSII), and non-photochemical quenching (NPQ) of four Rhododendrons were significantly higher in summer than in other seasons. The lower qP indicated the four Rhododendrons were susceptible to photoinhibition. Overall, the four Rhododendrons had similar physical characteristics in subalpine habitats. The parameters of the maximum quantum yield of photosystem II (PSII), the actual quantum yield of PSII, the non-photochemical chlorophyll fluorescence quenching, and chlorophyll a content increased in summer. Meanwhile, the protective enzyme activity and total soluble sugar content, proline content, and carotenoid content increased in spring, autumn, and winter. These results suggested that the four Rhododendrons can adapt to subalpine habitats by heat dissipation to avoid the damage of excessive radiation during the warm season while scavenging reactive oxygen and increasing the intracellular fluid concentration to avoid damage caused by chilling temperatures during the cold seasons. These findings would provide a reference for the conservation and application of these valuable ornamental evergreen broadleaf Rhododendrons, and enrich theory of plant eco-physiology in the high altitudes of the QTP.
Stimulation, Reduction and Compensation Growth, and Variable Phenological Responses to Spring and/or Summer–Autumn Warming in Corylus Taxa and Cornus sanguinea L.
Authors: Vander Mijnsbrugge Kristine , Malanguis Jessa May , Moreels Stefaan , Turcsán Arion , Paino Eduardo Notivol
Published: April 2022

Understanding species-specific responses to climate change allows a better assessment of the possible impact of global warming on forest growth. We studied the responses of the shrub species Corylus avellana L., Corylus maxima Mill. and intermediate forms, together stated as the Corylus taxa, and Cornus sanguinea L. upon periodically elevated temperatures in spring and/or in summer–autumn. Experiments were performed in a common garden, with Belgian and Pyrenean provenances for Corylus avellana and Cornus sanguinea. In the Corylus taxa, a warmer spring resulted in a reduction in height and diameter growth. Remarkably, the reduced diameter increment was restored with full compensation in the following year. The height increment for Cornus sanguinea was larger upon a warmer summer–autumn, concurring with a later leaf senescence. Our results suggest that Corylus is more sensitive to spring warming, influencing growth negatively, whereas Cornus is more sensitive to summer–autumn warming, influencing height growth positively. These deviating responses can be explained, at least partly, by their diverging ecological niches, with the Corylus taxa being more shade-tolerant compared to Cornus sanguinea. The warm spring conditions advanced bud burst in all studied taxa, whereas the warm summer–autumn advanced leaf senescence but prolonged its duration in the Corylus taxa, as well as delayed this phenophase in Cornus sanguinea. Little to no after-effects of the temperature treatments were detected. Although Corylus avellana and Cornus sanguinea plants originated from similar origins, their growth and phenological responses in the common garden diverged, with Corylus being more stable and Cornus displaying more variation between the Belgian and Pyrenean provenances.
Mangrove Forests in Ecuador: A Two-Decade Analysis
Authors: Morocho Ramiro , González Ivonne , Ferreira Tiago Osorio , Otero Xosé Luis
Published: April 2022

Mangroves are one of the most important ecosystems especially due to the services they provide, but in contrast are one of the most threatened by human activities at a global level. In Ecuador, mangrove forests are currently fragile and threatened due to the great anthropic pressure, which has largely reduced the area they occupy. However, there is already evidence that certain actions are contributing both to their conservation and the recovery of the lost mangrove area. In this study, we assessed the multitemporal dynamics of changes in mangrove cover in four coastal provinces of the country over a period of 20 years (1998–2018) based on remote sensing data analyzed using GIS tools. Our results showed that the area affected by mangrove forest destruction reached its maximum during the 1998–2010 period, when 4.56% (194.57 km2) of the mangrove forest was lost. This situation especially affected the provinces of El Oro and Guayas. The main cause for the loss of mangrove cover was the expansion of shrimp farms, followed by agriculture and construction. However, a slight recovery of ~2.9% has been observed, although loss remains constant. Mangrove ecosystem conservation policies, mainly applied to zones within protected areas; the establishment of use and custody agreements and the halt of shrimp farm expansion; the development of mangrove forests on areas with sediment deposits; and natural mangrove recovery processes are key factors for mangrove restoration. These results suggest that it is possible to continue restoring mangrove cover and thus maintain some of the main ecosystem services they provide for the benefit of humans.
Effects of Controlled Mycorrhization and Deficit Irrigation in the Nursery on Post-Transplant Growth and Physiology of Acer campestre L. and Tilia cordata Mill.
Authors: Corsini Denise , Vigevani Irene , Oggioni Silvio Daniele , Frangi Piero , Brunetti Cecilia , Mori Jacopo , Viti Carlo , Ferrini Francesco , Fini Alessio
Published: April 2022

The goal of this work was to assess the effects of mycorrhizal inoculation and deficit irrigation applied in the nursery on the post-transplant growth and physiology of Acer campestre L. and Tilia cordata Mill. For this purpose, 144 preconditioned plants were planted in an experimental plot in northern Italy and were monitored for three growing seasons. Controlled inoculation in the nursery enhanced the root colonization rate three years after transplanting only in Acer campestre. Inoculated Acer campestre showed higher survival, shoot length, turgor potential and leaf gas exchange than non-inoculated plants throughout the experiment. By contrast, in Tilia cordata, no difference in root colonization by mycorrhizal fungi was observed between plants inoculated or not in the nursery three years after transplanting. Indeed, the survival, growth and physiology of Tilia cordata after transplanting were little affected by inoculation. Deficit irrigation in the nursery determined higher survival, growth and CO2 assimilation rate and more favorable water relations in newly transplanted Acer campestre. By contrast, Tilia cordata exposed to deficit irrigation in the nursery showed lower growth and unaffected survival after transplanting compared to plants which received full irrigation in the nursery. The overall results suggest that nursery preconditioning through mycorrhizal inoculation and deficit irrigation can affect post-transplant performances, although their effectiveness depends on species’ mycorrhizal dependency and water use strategy.
Additive Root Biomass Equations for Betula platyphylla Suk. Plantations in Northeast China
Authors: Pan Li , Wang Xiuwei
Published: April 2022

Most of the forest biomass models that have been developed so far focus on the study of the aboveground biomass of forest trees and the prediction of belowground biomass remains obviously insufficient. Moreover, most of the existing studies on the estimation of the belowground biomass of trees have considered roots as a whole, ignoring the differences in composition and function of roots within different diameter classes. In this study, we measured the root biomass of birch plantation forests in northeastern China using extensive destructive sampling, in which we divided the root system into three parts: coarse, medium, and fine roots. We selected the best model base form from three common allometric biomass equations and determined the most appropriate error structure for the two sets of models using likelihood comparisons. The additive and disaggregated models were fitted using maximum likelihood with open-source software. We also added the site factor as a dummy variable into the two models. Finally, the competency of the two models was tested using ten-fold cross-validation. The results showed that both models could provide relatively accurate estimates of birch root biomass but that the disaggregated model performed slightly better than the additive model.
The Impact of Date Syrup on the Physicochemical, Microbiological, and Sensory Properties, and Antioxidant Activity of Bio-Fermented Camel Milk
Authors: Shahein Magdy Ramadan , Atwaa El Sayed Hassan , Elkot Wael F. , Hijazy Hayfa Hussin Ali , Kassab Rami B. , Alblihed Mohamed A. , Elmahallawy Ehab Kotb
Published: April 2022

Fermented camel milk is rich in nutrients and vitamins necessary for the health of humans and has therapeutic properties. Date palm camel milk has been reported to be beneficial for preventing and treating various diseases in Arab countries. This study targeted the production of probiotic fermented camel milk fortified with date syrup. In addition, the effect of adding date syrup as a prebiotic and flavoring agent to probiotic fermented camel milk on the physicochemical, phytochemical, microbiological, and sensory properties of probiotic fermented camel milk during storage was investigated. Probiotic fermented camel milk without adding date syrup served as a control, and the other two treatments were supplemented with date syrup at ratios of 6.0% and 8.0%. Probiotic fermented camel milk was analyzed after 1 day and 15 days from storage at 5 ± 1 °C. Interestingly, the present study revealed that the addition of date syrup significantly (p ≥ 0.05) increased total solids (TS), ash, Na, K, Fe, acetaldehyde, total phenolic contents, and titratable acidity, viscosity, and antioxidant values of resultant synbiotic fermented camel milk, and this increase was proportional to the level of date syrup fortification. In addition, non-significant changes in these components were observed during the storage period. However, total protein and fat content did not show significant changes. Furthermore, the addition of date syrup significantly increased (p ≥ 0.05) the total bacterial and Bifidobacteria counts, and this increase was associated with the level of the addition of date syrup. The addition of date syrup also significantly (p ≥ 0.05) improved the sensory scores for flavor, consistency, appearance, and total scores of resultant products. Moreover, the addition of date syrup at a level of 8% showed the highest sensory scores. In conclusion, probiotic fermented camel milk could be produced using a probiotic strain and flavored with date syrup at a level of 8%.
Nonlinear Waves Passing over Rectangular Obstacles: Multimodal Method and Experimental Validation
Authors: Monsalve Eduardo , Maurel Agnès , Pagneux Vincent , Petitjeans Philippe
Published: April 2022

We report a theoretical and experimental investigation of the propagation of nonlinear waves passing over a submerged rectangular step. A multimodal method allows calculating the first- and second-order reflected and transmitted waves. In particular, at the second order, the propagation of free and bound waves is theoretically presented. A detailed analysis of the convergence of the second-order problem shows that a finite truncation of the series of evanescent bound waves is necessary to obtain a smooth and convergent solution. The computed coefficients of the first and second harmonics are experimentally validated via a complete space-time-resolved measurements of the wave propagation, which permits us to verify the relative amplitude, phase and spatial interference (beating) of the free and bound waves at the second order. This result can be useful in future multimodal models since it not only keeps the accuracy of the model with the inclusion of the first part of the evanescent bound terms (being also the dominants) but also ensures the convergence of the multimodal computation with an error that decreases as a function of the number of modes.
A Simplified Mathematical Formulation for Water Quality Index (WQI): A Case Study in the Kelani River Basin, Sri Lanka
Authors: Makubura Randika , Meddage D. P. P. , Azamathulla Hazi Md. , Pandey Manish , Rathnayake Upaka
Published: April 2022

Surface water quality is degraded due to industrialization; however, it is one of the widely used sources for water supply systems worldwide. Thus, the polluted water creates significant issues for the health of the end users. However, poor attention and concern can be identified on this important issue in most developing countries, including Sri Lanka. The Kelani River in Sri Lanka is the heart of the water supply of the whole Colombo area and has the water intake for drinking purposes near an industrialized zone (Biyagama). Therefore, this study intends to analyze the effect of industrialization on surface water quality variation of the Kelani River basin in Sri Lanka in terms of the water quality index (WQI). We proposed a regression model to predict the WQI using the water quality parameters. Nine water quality parameters, including pH, total phosphate, electric conductivity, biochemical oxygen demand, temperature, nitrates, dissolved oxygen, chemical oxygen demand, and chlorine evaluated the Kelani River water quality. The proposed regression model was used to examine the water quality of samples obtained at twelve locations from January 2005 to December 2012. The highest WQI values were found in Raggahawatte Ela throughout the 8 years, located near the Biyagama industrial zone. The relationship of industries to water quality in the Kelani River is stated. The surface water quality gradually decreased as a result of development and industrialized activities. Therefore, this work showcases and recommends the importance of introducing necessary actions and considerations for future water management systems.
Optimization of Extraction Conditions to Improve Chlorogenic Acid Content and Antioxidant Activity of Extracts from Forced Witloof Chicory Roots
Authors: Chadni Morad , Isidore Emilie , Diemer Etienne , Ouguir Otmane , Brunois Fanny , Catteau Régis , Cassan Laurent , Ioannou Irina
Published: April 2022

Chlorogenic acids are major phenolic constituents in many herbal medicines and exhibit various bioactivities that explain the growing interest in extracting chlorogenic acids from biomass. In this context, the present study aims to maximize 3-O-Caffeoylquinic acid (3-CQA) and 3,5-O-di-caffeoylquinic acid (3,5-diCQA) contents from forced witloof chicory roots and to analyze the extraction kinetic modelling. First, the solid–liquid ratio, ethanol concentration, extraction time and temperature were studied. The extraction conditions were optimized to maximize the extraction of these compounds. The maximum yields reached 5 ± 0.11 and 5.97 ± 0.30 mg/g dry matter (DM) for 3-O-Caffeoylquinic acid and 3,5-O-di-caffeoylquinic acid, respectively, in less than 6 min at 70 °C. Extraction with water as a solvent was assessed with the aim of proposing a second greener and less-expensive solvent. This extraction is very fast from 90 °C, with a maximum of 6.22 ± 0.18 mg/gDM of 3-O-Caffeoylquinic acid, and instantaneous for 3,5-O-di-caffeoylquinic acid with a maximum of 6.44 ± 0.59 mg/gDM. In the second step, response surface methodology was employed to optimize the ultrasound-assisted extraction of antioxidants. The higher antioxidant activities were found at temperatures from 40 °C and at percentages of ethanol in the range of 35–70%.
In-Line Estimation of Fat Marbling in Whole Beef Striploins (Longissimus lumborum) by NIR Hyperspectral Imaging. A Closer Look at the Role of Myoglobin
Authors: Wold Jens Petter , Solberg Lars Erik , Gaarder Mari Øvrum , Carlehøg Mats , Sanden Karen Wahlstrøm , Rødbotten Rune
Published: April 2022

Fat marbling, the amount, and distribution of intramuscular fat, is an important quality trait for beef loin (Longissimus lumborum) and is closely connected to sensory properties such as tenderness, juiciness, and flavor. For meat producers, it would be of value to grade and sort whole loins according to marbling on the production line. The main goal of this study was to evaluate high-speed NIR hyperspectral imaging in interaction mode (760–1047 nm) for in-line measurement of sensory assessed marbling in both intact loins and loin slices. The NIR system was calibrated based on 28 whole striploins and 412 slices. Marbling scores were assessed for all slices on a scale from 1 to 9 by a trained sensory panel. The calibrated NIR system was tested for in-line measurements on 30 loins and 60 slices at a commercial meat producer. Satisfactory accuracy for prediction of marbling was obtained by partial least squares regression for both slices and whole loins (R2 = 0.81 & 0.82, RMSEP = 0.95 & 0.88, respectively). The concentration of myoglobin in the meat and its state of oxygenation has a strong impact on the NIR spectra and can give deviations in the estimated marbling scores. This must be carefully considered in industrial implementation.
Aronia melanocarpa Fruit Juice Modulates ACE2 Immunoexpression and Diminishes Age-Related Remodeling of Coronary Arteries in Rats
Authors: Daskalova Elena , Delchev Slavi , Vladimirova-Kitova Lyudmila , Bivolarski Iliya , Pencheva Mina , Denev Petko
Published: April 2022

The aim of the study is to evaluate the effect of Aronia melanocarpa fruit juice (AMJ) supplementation on age-related coronary arteries remodeled in aged rat hearts. Male Wistar rats (n = 24) were divided into three groups: (1) young controls (CY), aged 2 months, without AMJ supplementation; (2) old controls (CO), aged 27 months, without AMJ supplementation; and (3) the AMJ group (A), which used 27-month old animals, supplemented orally with AMJ for 105 days. AMJ supplementation did not influence the wall-to-diameter parameter (Kernohan index) of the coronary arteries of test animals. Aged rats supplemented with AMJ showed a significant decrease in the amount of collagen fibers in their coronary tunica media, as compared with the old controls. The intensity of the immunoreaction for alpha smooth muscle actin (αSMA) in the coronary tunica media was significantly lower in the supplemented group than in the old controls. The intensity of the angiotensin-converting enzyme 2 (ACE2) immunoreaction in the coronary tunica media of the supplemented group was significantly higher than the one observed in the old controls. These results indicate the positive effects of AMJ supplementation on the age-dependent remodeling of coronary arteries and support for the preventive potential of antioxidant-rich functional food supplementation in age-related diseases.
Map of a Bending Problem for Self-Similar Beams into the Fractal Continuum Using the Euler–Bernoulli Principle
Authors: Samayoa Ochoa Didier , Damián Adame Lucero , Kryvko Andriy
Published: April 2022

The bending of self-similar beams applying the Euler–Bernoulli principle is studied in this paper. A generalization of the standard Euler–Bernoulli beam equation in the FdH3 continuum using local fractional differential operators is obtained. The mapping of a bending problem for a self-similar beam into the corresponding problem for a fractal continuum is defined. Displacements, rotations, bending moments and shear forces as functions of fractal parameters of the beam are estimated, allowing the mechanical response for self-similar beams to be established. An example of the structural behavior of a cantilever beam with a load at the free end is considered to study the influence of fractality on the mechanical properties of beams.
Technical and Business Aspects of Battery Electric Trucks—A Systematic Review
Authors: Bhardwaj Shishir , Mostofi Hamid
Published: April 2022

Heavy-duty trucks (HDTs) are responsible for considerable fuel consumption and greenhouse gas emissions (GHG) in the road transportation sector due to their heavier weight, and significantly more miles travelled in comparison with other vehicles. Regarding the climate change mitigation policies, HDTs need to become zero-emission vehicles. One of the technological solutions in this sector is the battery electric truck (BET). This paper includes a systematic review on relevant studies in the field of BETs, including the following: (1) the technical, stakeholder, and customer aspects in terms of charging solutions to give a comprehensive insight into their technological advantages and disadvantages; (2) the total cost of ownership (TCO) for BETs and diesel trucks; and (3) a CO2 life cycle assessment (LCA) from different technologies. Moreover, the result is formulated in the form of SWOT analysis to describe the strengths, weaknesses, opportunities, and threats of different charging technologies. Moreover, the different calculation methods of the total cost of ownership for the heavy-duty battery trucks and diesel trucks are compared. In addition, the CO2 LCA is analyzed, and the different estimation methods of the CO2 released in the environment are compared, which includes the CO2 emissions during mobility operations and during the different manufacturing processes.
Radio Galaxies at TeV Energies
Authors: Rulten Cameron
Published: April 2022

Unlike blazars, radio galaxies have jets that are misaligned relative to our line-of-sight. This misaligned geometry provides us with a unique view of both the jet and super massive black hole. To date, four radio galaxies have been detected at TeV energies with an additional two active galactic nuclei shown to exhibit both radio galaxy and BL Lac-type properties. TeV observations of radio galaxies have revealed these objects to be fascinating, displaying ultra-fast variability and often relatively hard spectral energy distributions. This work aims to provide a review of the current state of radio galaxy observations within the context of very-high-energy γ-ray astronomy, while also highlighting that radio galaxies are excellent targets for multi-wavelength observations. A number of motivations for the continued study of radio galaxies are provided, and these are discussed with a focus on the key observational results, including implications for future observations with next-generation instruments soon to be operational.
Hydrogel-Based Adsorbent Material for the Effective Removal of Heavy Metals from Wastewater: A Comprehensive Review
Authors: Darban Zenab , Shahabuddin Syed , Gaur Rama , Ahmad Irfan , Sridewi Nanthini
Published: April 2022

Water is a vital resource that is required for social and economic development. A rapid increase in industrialization and numerous anthropogenic activities have resulted in severe water contamination. In particular, the contamination caused by heavy metal discharge has a negative impact on human health and the aquatic environment due to the non-biodegradability, toxicity, and carcinogenic effects of heavy metals. Thus, there is an immediate need to recycle wastewater before releasing heavy metals into water bodies. Hydrogels, as potent adsorbent materials, are a good contenders for treating toxic heavy metals in wastewater. Hydrogels are a soft matter formed via the cross-linking of natural or synthetic polymers to develop a three-dimensional mesh structure. The inherent properties of hydrogels, such as biodegradability, swell-ability, and functionalization, have made them superior applications for heavy metal removal. In this review, we have emphasized the recent development in the synthesis of hydrogel-based adsorbent materials. The review starts with a discussion on the methods used for recycling wastewater. The discussion then shifts to properties, classification based on various criteria, and surface functionality. In addition, the synthesis and adsorption mechanisms are explained in detail with the understanding of the regeneration, recovery, and reuse of hydrogel-based adsorbent materials. Therefore, the cost-effective, facile, easy to modify and biodegradable hydrogel may provide a long-term solution for heavy metal removal.
Rheological Investigation as Tool to Assess Physicochemical Stability of a Hyaluronic Acid Dermal Filler Cross-Linked with Polyethylene Glycol Diglycidyl Ether and Containing Calcium Hydroxyapatite, Glycine and L-Proline
Authors: Zerbinati Nicola , Capillo Maria Chiara , Sommatis Sabrina , Maccario Cristina , Alonci Giuseppe , Rauso Raffaele , Galadari Hassan , Guida Stefania , Mocchi Roberto
Published: April 2022

(1) Background: Dermal fillers are commonly used in aesthetic practice and their rheological characterization is of much interest today, as well as the stability study of the finished formula against external stimuli of a different nature (biological and physicochemical). Rheological tools have been exploited to characterize the physiochemical behaviour of a hyaluronic acid (HA) based dermal filler subjected to different thermal conditions over time. The collected results provide an index of its rheological stability. (2) Methods: After a preliminary Amplitude sweep test, the Frequency sweep test was performed in order to study the stability of a HA dermal filler cross-linked with Polyethylene Glycol Diglycidyl Ether (PEGDE) and containing Calcium Hydroxyapatite (CaHA), Glycine and L-Proline subjected to different conditions. Also, a shear rate ramp test was performed in order to investigate the filler’s flow behavior. (3) Results and Conclusions: G’ (elastic modulus), G’’ (viscous modulus) and consequentially tan δ (tangent of the phase angle) show a similar trend at different thermal conditions, underlining that the product is not affected by the storage conditions. The viscosity of the dermal filler decreases with an increasing shear rate, so a non-Newtonian shear thinning pseudoplastic behavior was demonstrated in all tested conditions.
Sensitive and Specific Detection of Lumpy Skin Disease Virus in Cattle by CRISPR-Cas12a Fluorescent Assay Coupled with Recombinase Polymerase Amplification
Authors: Jiang Chuanwen , Tao Dagang , Geng Yuanchen , Yang Hao , Xu Bingrong , Chen Yingyu , Hu Changmin , Chen Huanchun , Xie Shengsong , Guo Aizhen
Published: April 2022

Lumpy skin disease (LSD) is a severe and highly infectious pox disease of cattle caused by the lumpy skin disease virus (LSDV). To facilitate early control of LSD, this study aimed to develop a new rapid on-site LSDV detection method using an orf068 gene-based recombinase polymerase amplification assay (RPA) coupled with a CRISPR-Cas12a-based fluorescence assay (RPA-Cas12a-fluorescence assay). The results showed that the sensitivity of our RPA-Cas12a-fluorescence assay for detecting LSDV orf068 gene reached 5 copies/μL with plasmid as a template, and 102 TCID50/mL with viral genomic DNA as a template. No cross-reaction with other common bovine viruses was observed. Further, an on-site RPA-Cas12a-fluorescence assay of 40 clinical samples from cattle with or without LSD showed a diagnostic sensitivity of 96.3% (95% CI: 81.0–99.9%) and specificity of 92.31% (95% CI: 62.1–99.6%), which was close to those of the quantitative PCR assay. Therefore, our RPA-Cas12a-fluorescence assay has promising prospects in on-site rapid LSDV detection.
Development and Characterization of Chromosome Segment Substitution Lines Derived from Oryza rufipogon in the Background of the Oryza sativa indica Restorer Line R974
Authors: Ding Gumu , Hu Biaolin , Zhou Yi , Yang Wanling , Zhao Minmin , Xie Jiankun , Zhang Fantao
Published: April 2022

Dongxiang wild rice (DXWR) (O. rufipogon Griff.), which has the northernmost worldwide distribution of a wild rice species, is a valuable genetic resource with respect to improving stress tolerance in cultivated rice (Oryza sativa L.). In the three-line hybrid rice breeding system, restorer lines play important roles in enhancing the tolerance of hybrid rice. However, restorer lines have yet to be used as a genomic background for development of substitution lines carrying DXWR chromosome segments. We developed a set of 84 chromosome segment substitution lines (CSSLs) from a donor parent DXWR × recurrent parent restorer line R974 (Oryza sativa indica) cross. On average, each CSSL carried 6.27 introgressed homozygous segments, with 93.37% total genome coverage. Using these CSSLs, we identified a single QTL, qDYST-1, associated with salt stress tolerance on chromosome 3. Furthermore, five CSSLs showing strong salt stress tolerance were subjected to whole-genome single-nucleotide polymorphism chip analyses, during which we detected a common substitution segment containing qDYST-1 in all five CSSLs, thereby implying the validity and efficacy of qDYST-1. These novel CSSLs could make a significant contribution to detecting valuable DXWR QTLs, and provide important germplasm resources for breeding novel restorer lines for use in hybrid rice breeding systems.
Chimeric RNAs Discovered by RNA Sequencing and Their Roles in Cancer and Rare Genetic Diseases
Authors: Sun Yunan , Li Hui
Published: April 2022

Chimeric RNAs are transcripts that are generated by gene fusion and intergenic splicing events, thus comprising nucleotide sequences from different parental genes. In the past, Northern blot analysis and RT-PCR were used to detect chimeric RNAs. However, they are low-throughput and can be time-consuming, labor-intensive, and cost-prohibitive. With the development of RNA-seq and transcriptome analyses over the past decade, the number of chimeric RNAs in cancer as well as in rare inherited diseases has dramatically increased. Chimeric RNAs may be potential diagnostic biomarkers when they are specifically expressed in cancerous cells and/or tissues. Some chimeric RNAs can also play a role in cell proliferation and cancer development, acting as tools for cancer prognosis, and revealing new insights into the cell origin of tumors. Due to their abilities to characterize a whole transcriptome with a high sequencing depth and intergenically identify spliced chimeric RNAs produced with the absence of chromosomal rearrangement, RNA sequencing has not only enhanced our ability to diagnose genetic diseases, but also provided us with a deeper understanding of these diseases. Here, we reviewed the mechanisms of chimeric RNA formation and the utility of RNA sequencing for discovering chimeric RNAs in several types of cancer and rare inherited diseases. We also discussed the diagnostic, prognostic, and therapeutic values of chimeric RNAs.
An Adolescent Boy with Klinefelter Syndrome and 47,XXY/46,XX Mosaicism: Case Report and Review of Literature
Authors: Hovnik Tinka , Zitnik Eva , Avbelj Stefanija Magdalena , Bertok Sara , Sedej Katarina , Bancic Silva Vesna , Battelino Tadej , Groselj Urh
Published: April 2022

Klinefelter syndrome is the most commonly reported sex chromosome abnormality. It is heavily underdiagnosed due to the substantial variability of clinical presentations but is generally characterized by small, firm testes, hypergonadotropic hypogonadism, and the absence of spermatogenesis. Most patients with Klinefelter syndrome have a 47,XXY genotype. If they present with mosaicism, two different cell lines are usually identified, an aneuploid 47,XXY cell line and a normal male 46,XY cell line. There are very few cases of 47,XXY mosaicism with the additional female cell line 46,XX described in the literature. We report a case of an adolescent with the male phenotype and a rare variant mosaic 47,XXY/46,XX karyotype who presented with painless bilateral gynaecomastia. 47,XXY and 46,XX mosaic cell lines were identified with GTG-banding and further characterized using fluorescent in situ hybridization. We summarized the available clinical presentations of reported male patients with 47,XXY/46,XX mosaicism. To improve the clinical management and quality of life in individuals with rare and cryptic genomic imbalances, the genetic diagnosis would need to be extended to atypical cases.
Spatiotemporal Regulation of Circular RNA Expression during Liver Development of Chinese Indigenous Ningxiang Pigs
Authors: Chen Wenwu , Ma Haiming , Li Biao , Yang Fang , Xiao Yu , Gong Yan , Li Zhi , Li Ting , Zeng Qinghua , Xu Kang , Duan Yehui
Published: April 2022

Background: There have been many studies on the relationship between circRNAs and fat deposition. Although the liver is a central organ for fat metabolism, there are few reports on the relationship between circRNAs in the liver and fat deposition. Methods: In this study, we systematically analyzed circular RNAs in the liver of Ningxiang pigs, at four time points after birth (30 days, 90 days, 150 days and 210 days). Results: A total of 3705 circRNAs were coexpressed in four time periods were found, and KEGG analysis showed that the significantly upregulated pathways were mainly enriched in lipid metabolism and amino acid metabolism, while significantly downregulated pathways were mainly related to signal transduction, such as ECM–receptor interaction, MAPK signaling pathway, etc. Short time-series expression miner (STEM) analysis showed multiple model spectra that were significantly enriched over time in the liver. By constructing a competing endogenous RNA (ceRNA) regulatory network, 9187 pairs of networks related to the change in development time were screened. Conclusions: The expression profiles of circRNAs in Ningxiang pig liver were revealed at different development periods, and it was determined that there is differential coexpression. Through enrichment analysis of these circRNAs, it was revealed that host genes were involved in metabolism-related signaling pathways and fatty acid anabolism. Through STEM analysis, many circRNAs involved in fat metabolism, transport, and deposition pathways were screened, and the first circRNA–miRNA–mRNA regulation network map in Ningxiang pig liver was constructed. The highly expressed circRNAs related to fat deposition were verified and were consistent with RNA-Seq results.
Genetic Variants of MIR27A, MIR196A2 May Impact the Risk for the Onset of Coronary Artery Disease in the Pakistani Population
Authors: Haq Taqweem Ul , Zahoor Abdul , Ali Yasir , Chen Yangchao , Jalil Fazal , Shah Aftab Ali
Published: April 2022

Genetic variants in microRNA genes have a detrimental effect on miRNA-mediated regulation of gene expression and may contribute to coronary artery disease (CAD). CAD is the primary cause of mortality worldwide. Several environmental, genetic, and epigenetic factors are responsible for CAD susceptibility. The contribution of protein-coding genes is extensively studied. However, the role of microRNA genes in CAD is at infancy. The study is aimed to investigate the impact of rs895819, rs11614913, and rs2168518 variants in MIR27A, MIR196A2, and MIR4513, respectively, in CAD using allele-specific PCR. Results: For variant rs11614913, significant distribution of the genotypes among the cases and controls was determined by co-dominant [χ2 = 54.4; p value ≤ 0.0001], dominant (C/C vs. C/T + T/T) [OR = 0.257 (0.133–0.496); p value ≤ 0.0001], recessive (T/T vs. C/T + C/C) [OR = 1.56 (0.677–0.632); p value = 0.398], and additive models [OR = 0.421 (0.262–0.675); p value = 0.0004]. Similarly, a significant association of rs895819 was determined by co-dominant [χ2 = 9.669; p value ≤ 0.008], dominant (A/A vs. A/G + G/G) [OR = 0.285 (0.1242–0.6575); p value ≤ 0.0034], recessive (G/G vs. A/G + A/A) [OR = 0.900 (0.3202–3.519); p value = 1.000], and additive models [OR = 0.604 (0.3640–1.002); p value = 0.05] while no significant association of rs2168518 with CAD was found. Conclusion: The variants rs895819 and rs11614913 are the susceptibility factors for CAD.
A Practical Algorithm for the Viewpoint Planning of Terrestrial Laser Scanners
Authors: Jia Fengman , Lichti Derek D.
Published: April 2022

Applications using terrestrial laser scanners (TLS) have been skyrocketing in the past two decades. In a scanning project, the configuration of scans is a critical issue as it has significant effects on the project cost and the quality of the product. In this paper, a practical strategy is proposed to resolve the problem of the optimal placement of the terrestrial laser scanner. The method attempts to reduce the number of viewpoints under the premise that the scenes are fully covered. In addition, the approach is designed in a way that the solutions can be efficiently explored. The method has been tested on 540 polygons simulated with different sizes and complexities. The results have also been compared with a benchmark strategy in terms of the optimality of the solutions and runtime. It is concluded that our proposed algorithm ties or reduces the number of viewpoints in the benchmark paper in 85.6% of the 540 tests. For complex environments, the method can potentially reduce the project cost by 10%. Although with relatively lower efficiency, our method can still reach the solution within a few minutes for a polygon with up to 500 vertices.