bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-Wide Evolutionary Analysis of Putative Non-Specific Herbicide Resistance Genes and Compilation of Core Promoters between Monocots and Dicots
Authors: Chandra Saket , Leon Ramon G.
Published: June 2022

Herbicides are key weed-control tools, but their repeated use across large areas has favored the evolution of herbicide resistance. Although target-site has been the most prevalent and studied type of resistance, non-target-site resistance (NTSR) is increasing. However, the genetic factors involved in NTSR are widely unknown. In this study, four gene groups encoding putative NTSR enzymes, namely, cytochrome-P450, glutathione-S-transferase (GST), uridine 5′-diphospho-glucuronosyltransferase (UDPGT), and nitronate monooxygenase (NMO) were analyzed. The monocot and dicot gene sequences were downloaded from publicly available databases. Phylogenetic trees revealed that most of the CYP450 resistance-related sequences belong to CYP81 (5), and in GST, most of the resistance sequences belonged to GSTU18 (9) and GSTF6 (8) groups. In addition, the study of upstream promoter sequences of these NTSR genes revealed stress-related cis-regulatory motifs, as well as eight transcription factor binding sites (TFBS) were identified. The discovered TFBS were commonly present in both monocots and dicots, and the identified motifs are known to play key roles in countering abiotic stress. Further, we predicted the 3D structure for the resistant CYP450 and GST protein and identified the substrate recognition site through the homology approach. Our description of putative NTSR enzymes may be used to develop innovative weed control techniques to delay the evolution of NTSR.
Mediating Mindfulness-Based Interventions with Virtual Reality in Non-Clinical Populations: The State-of-the-Art
Authors: Failla Chiara , Marino Flavia , Bernardelli Luca , Gaggioli Andrea , Doria Germana , Chilà Paola , Minutoli Roberta , Mangano Rita , Torrisi Roberta , Tartarisco Gennaro , Bruschetta Roberta , Arcuri Francesco , Cerasa Antonio , Pioggia Giovanni
Published: June 2022

Mindfulness is one of the most popular psychotherapeutic techniques that help to promote good mental and physical health. Combining mindfulness with immersive virtual reality (VR) has been proven to be especially effective for a wide range of mood disorders for which traditional mindfulness has proven valuable. However, the vast majority of immersive VR-enhanced mindfulness applications have focused on clinical settings, with little evidence on healthy subjects. This narrative review evaluates the real effectiveness of state-of-the-art mindfulness interventions mediated by VR systems in influencing mood and physiological status in non-clinical populations. Only studies with an RCT study design were considered. We conclude that most studies were characterized by one single meditation experience, which seemed sufficient to induce a significant reduction in negative mood states (anxiety, anger, depression, tension) combined with increased mindfulness skills. However, physiological correlates of mindfulness practices have scarcely been investigated. The application of VR-enhanced mindfulness-based interventions in non-clinical populations is in its infancy since most studies have several limitations, such as the poor employment of the RCT study design, the lack of physiological measurements (i.e., heart rate variability), as well as the high heterogeneity in demographical data, technological devices, and VR procedures. We thus concluded that before applying mindfulness interventions mediated by VR in clinical populations, more robust and reliable methodological procedures need to be defined.
Evaluation of Parental Acceptability and Use of Intervention Components to Reduce Pre-School Children’s Intake of Sugar-Rich Food and Drinks
Authors: Christensen Bodil Just , Bestle Sidse Marie Sidenius , Trolle Ellen , Biltoft-Jensen Anja Pia , Matthiessen Jeppe , Gibbons Sarah Jegsmark , Lassen Anne Dahl
Published: June 2022

Knowledge is needed about effective tools that reach public health objectives focused on reducing the intake of sugar-rich foods and drinks. The purpose of this study was to assess the parental acceptability, use and motivational potential of intervention components developed in the randomized family-based trial ‘Are you too sweet?’ aimed at reducing the intake of sugar-rich foods and drinks among children (5–7 y). Intervention components included guidance on sugar-rich foods and drinks at a school health nurse consultation, a box with home-use materials and a digital platform. The methods used were a questionnaire among intervention families (n = 83) and semi-structured interviews with parents in selected intervention families (n = 24). Results showed the good acceptability and usefulness of the components, with reported frequencies of use of materials ranging from 48% to 94% and a high satisfaction rate with the school health nurse consultation. Personalized feedback and guidance from the school health nurse seemed to be a motivational trigger, and components that were compatible with existing practices were most frequently used. However, the components were not considered engaging by all families. Overall, intervention components were well received and hold the potential for enhancing parental knowledge and parenting practices regarding limiting the intake of sugar-rich foods and drinks.
Utilization of Random Forest Classifier and Artificial Neural Network for Predicting Factors Influencing the Perceived Usability of COVID-19 Contact Tracing “MorChana” in Thailand
Authors: Ong Ardvin Kester S. , Prasetyo Yogi Tri , Yuduang Nattakit , Nadlifatin Reny , Persada Satria Fadil , Robas Kirstien Paola E. , Chuenyindee Thanatorn , Buaphiban Thapanat
Published: June 2022

With the constant mutation of COVID-19 variants, the need to reduce the spread should be explored. MorChana is a mobile application utilized in Thailand to help mitigate the spread of the virus. This study aimed to explore factors affecting the actual use (AU) of the application through the use of machine learning algorithms (MLA) such as Random Forest Classifier (RFC) and Artificial Neural Network (ANN). An integrated Protection Motivation Theory (PMT) and the Unified Theory of Acceptance and Use of Technology (UTAUT) were considered. Using convenience sampling, a total of 907 valid responses from those who answered the online survey were voluntarily gathered. With 93.00% and 98.12% accuracy from RFC and ANN, it was seen that hedonic motivation and facilitating conditions were seen to be factors affecting very high AU; while habit and understanding led to high AU. It was seen that when people understand the impact and causes of the COVID-19 pandemic’s aftermath, its severity, and also see a way to reduce it, it would lead to the actual usage of a system. The findings of this study could be used by developers, the government, and stakeholders to capitalize on using the health-related applications with the intention of increasing actual usage. The framework and methodology used presented a way to evaluate health-related technologies. Moreover, the developing trends of using MLA for evaluating human behavior-related studies were further justified in this study. It is suggested that MLA could be utilized to assess factors affecting human behavior and technology used worldwide.
Potential Common Mechanisms of Cytotoxicity Induced by Amide Herbicides via TRPA1 Channel Activation
Authors: Wang Xiaoning , Sun Yangyang , Wang Qian , Liu Fengying , Yang Weijie , Sui Xin , Yang Jun , Zhang Minmin , Wang Shuai , Xiao Zhenyu , Luo Yuan , Wang Yongan , Zhu Tong
Published: June 2022

The “Multi-Threat Medical Countermeasure (MTMC)” strategy was proposed to develop a single drug with therapeutic efficacy against multiple pathologies or broad-spectrum protection against various toxins with common biochemical signals, molecular mediators, or cellular processes. This study demonstrated that cytotoxicity, expression of transient receptor potential cation channel subfamily A member 1 (TRPA1) mRNA, and intracellular calcium influx were increased in A549 cells exposed to amide herbicides (AHs), in which the order of cytotoxicity was metolachlor > acetochlor > propisochlor > alachlor > butachlor > propanil > pretilachlor, based on IC50 values of 430, 524, 564, 565, 619, 831, and 2333 μM, respectively. Inhibition/knockout of TRPA1 efficiently protected against cytotoxicity, decreased TRPA1 mRNA expression, and reduced calcium influx. The results suggested that the TRPA1 channel could be a key common target for AHs poisoning. The order of TRPA1 affinity for AHs was propanil > pretilachlor > metolachlor > (propiso/ala/aceto/butachlor), based on KD values of 16.2, 309, and 364 μM, respectively. The common molecular mechanisms of TRPA1-AHs interactions were clarified, including toxicity-effector groups (benzene ring, nitrogen/oxygen-containing functional groups, halogen) and residues involved in interactions (Lys787, Leu982). This work provides valuable information for the development of TRPA1 as a promising therapeutic target for broad-spectrum antitoxins.
Conflict Identification and Zoning Optimization of “Production-Living-Ecological” Space
Authors: Xiao Pengnan , Xu Jie , Zhao Chong
Published: June 2022

With the acceleration of economic and social development and the increasing competition between multi-functional spaces, the coordination and stability of land space have been seriously affected. In order to simulate the conflict pattern of “production, living ecological” space and analyze its evolution characteristics, taking Qianjiang City as the research area and based on the current data of land use, the FLUS (Future Land Use Simulation) model and spatial conflict measurement model are used to calculate the change trend of “production, living ecological” spatial conflict in Qianjiang City in the past and in the future. The research results are of great significance for the scientific use of land space and the optimization of regional development patterns. The results show that: (1) From 2000 to 2020, the level of spatial conflict in Qianjiang City showed an upward trend, the proportion of medium and above conflict units gradually increased, and the conflict level in the study area gradually became dominated by strong conflict. (2) Due to the process of urbanization and the continuous growth of population and GDP (Gross Domestic Product), the construction land in Qianjiang City shows a rapid increase trend under three scenarios, and the cultivated land area shows a downward trend. (3) In 2035, under the three scenarios, the spatial conflict in Qianjiang City will be strengthened, mainly at the level of medium and above. (4) According to the change degree of conflict transformation, 15 change types are divided into five functional zones: ecological protection zone, ecological conservation zone, modern agriculture zone, urban–rural development coordination zone and urban optimization zone.
Implementation of Virtual Communities of Practice in Healthcare to Improve Capability and Capacity: A 10-Year Scoping Review
Authors: Shaw Louise , Jazayeri Dana , Kiegaldie Debra , Morris Meg E.
Published: June 2022

Virtual communities of practice consist of individuals who use a common online platform to share professional expertise and experiences. In healthcare settings a virtual community of practice (VCoP) can optimise knowledge, skills, and the implementation of evidence-based practice. To ensure effective knowledge synthesis and translation into practice, it is essential to clarify the best methods for designing and implementing VCoPs within healthcare organisations. This scoping review aimed to identify the methods used to establish and facilitate online or digitally enabled communities of practice within healthcare organisations across the globe. Six online databases identified papers published from January 2010 to October 2020. Papers were independently screened by two reviewers using Covidence. Data were captured and analysed using a data extraction chart in Covidence. Twenty-four publications that detail methods for establishing a VCoP in healthcare were included. Few studies used a framework to establish a VCoP. It was difficult to identify details regarding methods of development and key elements such as roles, how they were coordinated, and types of technology used. Healthcare organisations can benefit from using a standardised framework for the establishment, implementation and evaluation of VCoPs to improve practice, staff engagement, and knowledge sharing.
Evaluation of a Follow-Up Health Consultation Program for Patients with Coronavirus Disease 2019 in Korea: Using the Context–Input–Process–Product Model
Authors: Lee Keun-Mi , Ko Hae-Jin , Lee Geon Ho , Kim Yun-A , Jung Seung-Pil , Kim A-Sol
Published: June 2022

Beyond physical pain, patients with coronavirus disease 2019 (COVID-19) experience psychological anxiety during and after quarantine, often facing negative perceptions when returning to their communities. This study evaluated a health consultation program in Korea for post-quarantine patients with COVID-19, designed to help them return to their communities. The program was conducted from 9 March to 5 June 2020, in Daegu, Korea. In total, 20 doctors and 504 recovered patients were surveyed via questionnaire. The survey, comprising open-ended questions rated on a five-point Likert scale, was based on the Context–Input–Process–Product program evaluation model. Reliability was assessed, and descriptive statistics were obtained. A regression analysis was performed on factors affecting product (output) areas. As a main result, both doctors and recovered patients evaluated the program positively. The mean program effectiveness score was 4.00 in the doctors’ evaluations and 3.95 in the patients’ evaluations. Moreover, the input and process variables affected the product. This first-of-its-kind health consultation program proved to be an effective practical intervention for patients returning to the community after an infectious disease; it also highlights aspects that could increase satisfaction in systemized subsequent programs, with input and process areas for patients and doctors.
Green Jobs—A Literature Review
Authors: Stanef-Puică Mihaela-Roberta , Badea Liana , Șerban-Oprescu George-Laurențiu , Șerban-Oprescu Anca-Teodora , Frâncu Laurențiu-Gabriel , Crețu Alina
Published: June 2022

In the past two decades the topic of “green jobs” has drawn particular attention, resulting in a widely diverse and relatively large number of published papers. Although a determinant for the increase of knowledge, the heterogeneity of these studies may raise the issue of a systematic documentation of the key contributions in this field. In this context, the present research focuses on analyzing the scientific literature published in the last five years on the topic of “green jobs” with the aim to identify definitions and meanings associated with the concept of “green jobs”, the connected terms, areas of research interest and the main theoretical and practical results. The results reveal that although there is no uniformity in the definition of the concept, there is still a convergence towards the initial meaning offered by the UNEP/ILO/IOE/ITUC Report of 2008. Moreover, using scientific software VOSViewer our research shows that the concept of “green jobs” is most linked with the following terms: sustainable development, the green economy, the circular economy, the welfare economy, the European Green Pact, energy, renewable energy, economic development, and employment. Furthermore, our analysis reveals that the studies focused on “green jobs” are mainly concerned with the following issues: green jobs creation, work-life balance, correlations between green business and green jobs and the role of local government in supporting green jobs.
The Impact of Online Environmental Platform Services on Users’ Green Consumption Behaviors
Authors: Ma Yuan , Liu Changshan
Published: June 2022

With the continuous prominence of environmental problems, some online environmental platforms have been built in China. Such platforms provide an important carrier for public to learn environmental knowledge and participate in environmental protection. However, whether such platforms can play a substantive role in promoting users’ green consumption behaviors is still unclear. Focusing on this question, the influence of online environmental platform services on public green consumption behaviors is explored. A model based on the theory of stimulus–organism–response is established to analyze the influential mechanism, using the online environmental platform services as the independent variable, users’ green consumption behaviors as the dependent variable, environmental attitude as the mediator, and users’ price sensitivity as the moderator. Survey data are used to test the model. The empirical results show that online environmental platform services have a significant positive impact on users’ green consumption behaviors. Environmental attitude plays a partial mediating role and price sensitivity negatively moderates the mediating role of environmental attitude. Suggestions are given from the perspectives of platform operators and government. This paper provides both theoretical and practical implications for sustainable consumption.
Sub-Surface Geotechnical Data Visualization of Inaccessible Sites Using GIS
Authors: Awan Tariq Ahmed , Arshid Muhammad Usman , Riaz Malik Sarmad , Houda Moustafa , Abdallah Mirvat , Shahkar Muhammad , Aghdam Mirsina Mousavi , Azab Marc
Published: June 2022

Geotechnical investigation, in hilly areas, for high-rise projects, becomes a problematic issue and costly process due to difficulties in mobilization and assembling the drilling equipment on mountainous terrains. The objective of this study is to map soil properties of study areas, especially at inaccessible sites, for reconnaissance. Digital soil maps for Tehsil Murree, Pakistan, have been developed using the emerging Geographical Information System (GIS). The research work involved the creation of an exhaustive database, by collecting and rectifying geotechnical data, followed by the digitization of the acquired data through integration with GIS, in an attempt to visualize, analyze and interpret the collected geotechnical information spatially. The soil data of 205 explanatory holes were collected from the available geotechnical investigation (GI) reports. The collection depth of soil samples, which were initially used for the design of deep and shallow foundations by different soil consultancies in the Murree area, was approximately 50 ft. below ground level. Appropriate spatial interpolation methods (i.e., the Kriging) were applied for the preparation of smooth surface maps of soil standard penetration tester number values, soil type and plasticity index. The accuracy of developed SPT N value and plasticity maps were then evaluated using the linear regression method, in which the predicted values of soil characteristics from developed maps and actual values were compared. SPT N value maps were developed up to a depth of 9.14 m below ground level and at every 1.52 m interval. The depth of refusal was considered in the developed maps. Soil type and plasticity maps were generated up to 15.24 m depth, again at every 1.52 m intervals, using color contours, considering the maximum predicted foundation depth for high-rise projects. The study has implications for academics and practitioners to map the soil properties for inaccessible sites using GIS, as the resulting maps have high accuracy.
Cancer Cell Metabolism
Authors: Parkinson Eric K. , Haferkamp Sebastian , Mycielska Maria E.
Published: June 2022

Cancer metabolism has been of interest for decades; however, the recent development of sophisticated techniques such as metabolomics or lipidomics have significantly increased our understanding of processes taking place in tumour cells [...]
Novel Insights into Factor D Inhibition
Authors: Gavriilaki Eleni , Papakonstantinou Anna , Agrios Konstantinos A.
Published: June 2022

Complement-mediated diseases or complementopathies, such as Paroxysmal nocturnal hemoglobinuria (PNH), cold agglutinin disease (CAD), and transplant-associated thrombotic microangiopathy (TA-TMA), demand advanced complement diagnostics and therapeutics be adopted in a vast field of medical specialties, such as hematology, transplantation, rheumatology, and nephrology. The miracle of complement inhibitors as “orphan drugs” has dramatically improved morbidity and mortality in patients with otherwise life-threatening complementopathies. Efficacy has been significantly improved by upstream inhibition in patients with PNH. Different molecules may exert diverse characteristics in vitro and in vivo. Further studies remain to show safety and efficacy of upstream inhibition in other complementopathies. In addition, cost and availability issues are major drawbacks of current treatments. Therefore, further developments are warranted to address the unmet clinical needs in the field of complementopathies. This state-of-the-art narrative review aims to delineate novel insights into factor D inhibition as a promising target for complementopathies.
Targeted Therapy and Immunotherapy in Early-Stage Non-Small Cell Lung Cancer: Current Evidence and Ongoing Trials
Authors: de Scordilli Marco , Michelotti Anna , Bertoli Elisa , De Carlo Elisa , Del Conte Alessandro , Bearz Alessandra
Published: June 2022

The scenario of neoadjuvant and adjuvant settings in non-small cell lung cancer (NSCLC) is rapidly evolving. As already happened for the advanced disease, also early stages have entered the era of precision medicine, with molecular analysis and Programmed death-ligand 1 (PD-L1) evaluation that by now can be considered a routine assessment. New treatment options have been recently approved, with osimertinib now part of clinical practice for Epidermal Growth Factor Receptor mutated (EGFRm) patients, and immune checkpoint inhibitors (ICIs) available after FDA approval both in the adjuvant (atezolizumab) and neoadjuvant (nivolumab) setting. No mature data on overall survival benefits are available yet, though. Several clinical trials with specific-tyrosine kinase inhibitors (TKIs) and ICIs are currently ongoing, both with and without concomitant chemotherapy. As therapeutic strategies are rapidly expanding, quite a few questions remain unsettled, such as the optimal duration of adjuvant targeted therapy or the effective benefit of ICIs in early-stage EGFRm or ALK (Anaplastic Lymphoma Kinase) rearranged patients, or the possibility to individuate high-risk patients after surgical resection assessing minimal residual disease (MRD) by ctDNA evaluation. We hereby report already available literature data and summarize ongoing trials with targeted therapy and immunotherapy in early-stage NSCLC, focusing on practice-changing results and new perspectives for potentially cured patients.
Inhibition of Recruitment and Activation of Neutrophils by Pyridazinone-Scaffold-Based Compounds
Authors: Moniot Aurélie , Braux Julien , Siboni Renaud , Guillaume Christine , Audonnet Sandra , Allart-Simon Ingrid , Sapi Janos , Tirouvanziam Rabindra , Gérard Stéphane , Gangloff Sophie C. , Velard Frédéric
Published: June 2022

In inflammatory diseases, polymorphonuclear neutrophils (PMNs) are known to produce elevated levels of pro-inflammatory cytokines and proteases. To limit ensuing exacerbated cell responses and tissue damage, novel therapeutic agents are sought. 4aa and 4ba, two pyridazinone-scaffold-based phosphodiesterase-IV inhibitors are compared in vitro to zardaverine for their ability to: (1) modulate production of pro-inflammatory mediators, reactive oxygen species (ROS), and phagocytosis; (2) modulate degranulation by PMNs after transepithelial lung migration. Compound 4ba and zardaverine were tested in vivo for their ability to limit tissue recruitment of PMNs in a murine air pouch model. In vitro treatment of lipopolysaccharide-stimulated PMNs with compounds 4aa and 4ba inhibited the release of interleukin-8, tumor necrosis factor-α, and matrix metalloproteinase-9. PMNs phagocytic ability, but not ROS production, was reduced following treatment. Using a lung inflammation model, we proved that PMNs transmigration led to reduced expression of the CD16 phagocytic receptor, which was significantly blunted after treatment with compound 4ba or zardaverine. Using the murine air pouch model, LPS-induced PMNs recruitment was significantly decreased upon addition of compound 4ba or zardaverine. Our data suggest that new pyridazinone derivatives have therapeutic potential in inflammatory diseases by limiting tissue recruitment and activation of PMNs.
3-keto-DON, but Not 3-epi-DON, Retains the in Planta Toxicological Potential after the Enzymatic Biotransformation of Deoxynivalenol
Authors: Li Xiu-Zhen , Hassan Yousef I. , Lepp Dion , Zhu Yan , Zhou Ting
Published: June 2022

Deoxynivalenol (DON) is a secondary fungal metabolite that is associated with many adverse toxicological effects in agriculture as well as human/animal nutrition. Bioremediation efforts in recent years have led to the discovery of numerous bacterial isolates that can transform DON to less toxic derivatives. Both 3-keto-DON and 3-epi-DON were recently shown to exhibit reduced toxicity, compared to DON, when tested using different cell lines and mammalian models. In the current study, the toxicological assessment of 3-keto-DON and 3-epi-DON using in planta models surprisingly revealed that 3-keto-DON, but not 3-epi-DON, retained its toxicity to a large extent in both duckweeds (Lemna minor L.) and common wheat (Triticum aestivum L.) model systems. RNA-Seq analysis revealed that the exposure of L. minor to 3-keto-DON and DON resulted in substantial transcriptomic changes and similar gene expression profiles, whereas 3-epi-DON did not. These novel findings are pivotal for understanding the environmental burden of the above metabolites as well as informing the development of future transgenic plant applications. Collectively, they emphasize the fundamental need to assess both plant and animal models when evaluating metabolites/host interactions.
Unusual Post-Translational Modifications in the Biosynthesis of Lasso Peptides
Authors: Duan Yuwei , Niu Weijing , Pang Linlin , Bian Xiaoying , Zhang Youming , Zhong Guannan
Published: June 2022

Lasso peptides are a subclass of ribosomally synthesized and post-translationally modified peptides (RiPPs) and feature the threaded, lariat knot-like topology. The basic post-translational modifications (PTMs) of lasso peptide contain two steps, including the leader peptide removal of the ribosome-derived linear precursor peptide by an ATP-dependent cysteine protease, and the macrolactam cyclization by an ATP-dependent macrolactam synthetase. Recently, advanced bioinformatic tools combined with genome mining have paved the way to uncover a rapidly growing number of lasso peptides as well as a series of PTMs other than the general class-defining processes. Despite abundant reviews focusing on lasso peptide discoveries, structures, properties, and physiological functionalities, few summaries concerned their unique PTMs. In this review, we summarized all the unique PTMs of lasso peptides uncovered to date, shedding light on the related investigations in the future.
Deletion of Diterpenoid Biosynthetic Genes CYP76M7 and CYP76M8 Induces Cell Death and Enhances Bacterial Blight Resistance in Indica Rice ‘9311’
Authors: Jiang Min , Yu Ning , Zhang Yingxin , Liu Lin , Li Zhi , Wang Chen , Cheng Shihua , Cao Liyong , Liu Qunen
Published: June 2022

Lesion mimic mutants (LMMs) are ideal materials for studying cell death and resistance mechanisms. Here, we identified and mapped a novel rice LMM, g380. The g380 exhibits a spontaneous hypersensitive response-like cell death phenotype accompanied by excessive accumulation of reactive oxygen species (ROS) and upregulated expression of pathogenesis-related genes, as well as enhanced resistance to Xanthomonas oryzae pv. oryzae (Xoo). Using a map-based cloning strategy, a 184,916 bp deletion on chromosome 2 that overlaps with the diterpenoid biosynthetic gene cluster was identified in g380. Accordingly, the content of diterpenoids decreased in g380. In addition, lignin, one of the physical lines of plant defense, was increased in g380. RNA-seq analysis showed 590 significantly differentially expressed genes (DEG) between the wild-type 9311 and g380, 585 of which were upregulated in g380. Upregulated genes in g380 were mainly enriched in the monolignol biosynthesis branches of the phenylpropanoid biosynthesis pathway, the plant–pathogen interaction pathway and the phytoalexin-specialized diterpenoid biosynthesis pathway. Taken together, our results indicate that the diterpenoid biosynthetic gene cluster on chromosome 2 is involved in immune reprogramming, which in turn regulates cell death in rice.
Editorial: Tropical Plant Responses to Climate Change
Authors: Marques Isabel , Ribeiro-Barros Ana , Ramalho José Cochicho
Published: June 2022

The climate crisis is pushing the planet’s tropical plants towards their limits [...]
Searching for a Paradigm Shift in Auger-Electron Cancer Therapy with Tumor-Specific Radiopeptides Targeting the Mitochondria and/or the Cell Nucleus
Authors: Fernandes Célia , Palma Elisa , Silva Francisco , Belchior Ana , Pinto Catarina I. G. , Guerreiro Joana F. , Botelho Hugo M. , Mendes Filipa , Raposinho Paula , Paulo António
Published: June 2022

Although 99mTc is not an ideal Auger electron (AE) emitter for Targeted Radionuclide Therapy (TRT) due to its relatively low Auger electron yield, it can be considered a readily available “model” radionuclide useful to validate the design of new classes of AE-emitting radioconjugates. With this in mind, we performed a detailed study of the radiobiological effects and mechanisms of cell death induced by the dual-targeted radioconjugates 99mTc-TPP-BBN and 99mTc-AO-BBN (TPP = triphenylphosphonium; AO = acridine orange; BBN = bombesin derivative) in human prostate cancer PC3 cells. 99mTc-TPP-BBN and 99mTc-AO-BBN caused a remarkably high reduction of the survival of PC3 cells when compared with the single-targeted congener 99mTc-BBN, leading to an augmented formation of γH2AX foci and micronuclei. 99mTc-TPP-BBN also caused a reduction of the mtDNA copy number, although it enhanced the ATP production by PC3 cells. These differences can be attributed to the augmented uptake of 99mTc-TPP-BBN in the mitochondria and enhanced uptake of 99mTc-AO-BBN in the nucleus, allowing the irradiation of these radiosensitive organelles with the short path-length AEs emitted by 99mTc. In particular, the results obtained for 99mTc-TPP-BBN reinforce the relevance of targeting the mitochondria to promote stronger radiobiological effects by AE-emitting radioconjugates.
A Nuclear Factor Y-B Transcription Factor, GmNFYB17, Regulates Resistance to Drought Stress in Soybean
Authors: Sun Maolin , Li Yue , Zheng Jiqiang , Wu Depeng , Li Chunxia , Li Zeyang , Zang Ziwei , Zhang Yanzheng , Fang Qingwei , Li Wenbin , Han Yingpeng , Zhao Xue , Li Yongguang
Published: June 2022

Soybean is sensitive to drought stress, and increasing tolerance to drought stresses is an important target for improving the performance of soybean in the field. The genetic mechanisms underlying soybean’s drought tolerance remain largely unknown. Via a genome-wide association study (GWAS) combined with linkage analysis, we identified 11 single-nucleotide polymorphisms (SNPs) and 22 quantitative trait locus (QTLs) that are significantly associated with soybean drought tolerance. One of these loci, namely qGI10-1, was co-located by GWAS and linkage mapping. The two intervals of qGI10-1 were differentiated between wild and cultivated soybean. A nuclear factor Y transcription factor, GmNFYB17, was located in one of the differentiated regions of qGI10-1 and thus selected as a candidate gene for further analyses. The analysis of 29 homologous genes of GmNFYB17 in soybean showed that most of the genes from this family were involved in drought stress. The over-expression of GmNFYB17 in soybean enhanced drought resistance and yield accumulation. The transgenic plants grew better than control under limited water conditions and showed a lower degree of leaf damage and MDA content but higher RWC, SOD activity and proline content compared with control. Moreover, the transgenic plants showed a fast-growing root system, especially regarding a higher root–top ratio and more branching roots and lateral roots. The better agronomic traits of yield were also found in GmNFYB17 transgenic plants. Thus, the GmNFYB17 gene was proven to positively regulate drought stress resistance and modulate root growth in soybean. These results provide important insights into the molecular mechanisms underlying drought tolerance in soybean.
Identification and Fine Mapping of the Candidate Gene Controlling Multi-Inflorescence in Brassica napus
Authors: Lu Hongchen , Wu Hanfei , Zhu Guangfeng , Yin Caijun , Zhao Lun , Wen Jing , Yi Bin , Ma Chaozhi , Tu Jinxing , Fu Tingdong , Shen Jinxiong
Published: June 2022

As a desirable agricultural trait, multi-inflorescence (MI) fulfills the requirement of mechanized harvesting and yield increase in rapeseed (Brassica napus L.). However, the genetic mechanism underlying the multi-inflorescence trait remain poorly understood. We previously identified a difference of one pair of dominant genes between the two mapping parental materials. In this study, phenotype and expression analysis indicated that the imbalance of the CLAVATA (CLV)-WUSCHEL (WUS) feedback loop may contribute to the abnormal development of the shoot apical meristem (SAM). BnaMI was fine-mapped to a 55 kb genomic region combining with genotype and phenotype of 5768 BCF1 individuals using a traditional mapping approach. Through comparative and expression analyses, combined with the annotation in Arabidopsis, five genes in this interval were identified as candidate genes. The present findings may provide assistance in functional analysis of the mechanism associated with multi-inflorescence and yield increase in rapeseed.
LIN28 Family in Testis: Control of Cell Renewal, Maturation, Fertility and Aging
Authors: Krsnik Dajana , Marić Tihana , Bulić-Jakuš Floriana , Sinčić Nino , Bojanac Ana Katušić
Published: June 2022

Male reproductive development starts early in the embryogenesis with somatic and germ cell differentiation in the testis. The LIN28 family of RNA-binding proteins promoting pluripotency has two members—LIN28A and LIN28B. Their function in the testis has been investigated but many questions about their exact role based on the expression patterns remain unclear. LIN28 expression is detected in the gonocytes and the migrating, mitotically active germ cells of the fetal testis. Postnatal expression of LIN28 A and B showed differential expression, with LIN28A expressed in the undifferentiated spermatogonia and LIN28B in the elongating spermatids and Leydig cells. LIN28 interferes with many signaling pathways, leading to cell proliferation, and it is involved in important testicular physiological processes, such as cell renewal, maturation, fertility, and aging. In addition, aberrant LIN28 expression is associated with testicular cancer and testicular disorders, such as hypogonadotropic hypogonadism and Klinefelter’s syndrome. This comprehensive review encompasses current knowledge of the function of LIN28 paralogs in testis and other tissues and cells because many studies suggest LIN28AB as a promising target for developing novel therapeutic agents.
Detection of Human Papillomavirus in Squamous Lesions of the Conjunctiva Using RNA and DNA In-Situ Hybridization
Authors: Peterson Cornelia , Parikh Rupin N. , Ahmad Meleha T. , Campbell Ashley A. , Daoud Yassine , Mahoney Nicholas , Siadati Sepideh , Eberhart Charles G.
Published: June 2022

In-situ hybridization provides a convenient and reliable method to detect human papillomavirus (HPV) infection in formalin-fixed paraffin-embedded tissue. Cases of conjunctival papillomas, conjunctival intraepithelial neoplasia (CIN), conjunctival carcinoma in situ (cCIS), and invasive squamous cell carcinoma (SCC), in which low-risk (LR) and/or high-risk (HR) HPV types were evaluated by RNA or DNA in-situ hybridization, were retrospectively identified. LR HPV types were frequently detected in conjunctival papillomas (25/30, 83%), including 17/18 (94%) with RNA probes, compared to 8/12 (75%) with DNA probes. None of the CIN/cCIS or SCC cases were positive for LR HPV by either method. HR HPV was detected by RNA in-situ hybridization in 1/16 (6%) of CIN/cCIS cases and 2/4 (50%) of SCC cases, while DNA in-situ hybridization failed to detect HPV infection in any of the CIN/cCIS lesions. Reactive atypia and dysplasia observed in papillomas was generally associated with the detection of LR HPV types. Collectively, our findings indicate RNA in-situ hybridization may provide a high-sensitivity approach for identifying HPV infection in squamous lesions of the conjunctiva and facilitate the distinction between reactive atypia and true dysplasia. There was no clear association between HPV infection and atopy in papillomas or dysplastic lesions.
Research Progress on the Preparation and High-Value Utilization of Lignin Nanoparticles
Authors: Liu Kefeng , Zhuang Yuntang , Chen Jiachuan , Yang Guihua , Dai Lin
Published: June 2022

Lignin nanoparticles, the innovative achievements in the development and utilization of lignin, combine the structural characteristics of nanomaterials and lignin molecules and have a wide range of applications. In this review, we summarize the methods for preparing lignin nanoparticles by solvent exchange method, mechanical method, biological enzymatic method, interface polymerization/crosslinking method, and spray freezing method, and emphatically introduce the application prospects of lignin nanoparticles in ultraviolet protection, antibacterial, nano-filler, drug delivery, and adsorption, aiming to provide a certain reference direction for additional high-value applications of lignin nanoparticles.
Anxiety-like Behavior and GABAAR/BDZ Binding Site Response to Progesterone Withdrawal in a Stress-Vulnerable Strain, the Wistar Kyoto Rats
Authors: Islas-Preciado Dannia , Ugalde-Fuentes Gabriela , Sollozo-Dupont Isabel , González Trujano María Eva , Cervantes-Anaya Nancy , Estrada-Camarena Erika , López-Rubalcava Carolina
Published: June 2022

Stress susceptibility could play a role in developing premenstrual anxiety due to abnormalities in the hypothalamus–pituitary–adrenal (HPA) axis and impairments in the GABAA receptors’ benzodiazepine (BDZ) site. Hence, we studied the stress-vulnerable Wistar Kyoto rat strain (WKY) to evaluate progesterone withdrawal (PW) effects on anxiety, HPA axis response, and to explore indicators of GABAA functionality in the BDZ site. For five days, ovariectomized WKY rats were administered 2.0 mg/kg of progesterone. Twenty-four hours after the last administration, rats were tested in the anxiety-like burying behavior test (BBT) or elevated plus maze test (EPM), and corticosterone was determined. [3H]Flunitrazepam binding autoradiography served as the BDZ binding site index of the GABAA receptor in amygdala nuclei and hippocampus’s dentate gyrus (DG). Finally, different doses of diazepam in PW-WKY rats were tested in the BBT. PW induced anxiety-like behaviors in both BBT and EPM compared with No-PW rats. PW increased corticosterone, but was blunted when combined with PW and BBT. PW increased [3H]Flunitrazepam binding in the DG and central amygdala compared with No-PW rats. Diazepam at a low dose induced an anxiogenic-like response in PW rats, suggesting a paradoxical response to benzodiazepines. Overall, PW induced anxiety-like behavior, a blunted HPA axis response, and higher GABAAR/BZD binding site sensitivity in a stress-vulnerable rat strain. These findings demonstrate the role of stress-susceptibility in GABAAR functionality in a preclinical approximation of PMDD.
A Non-Iterative Constrained Measure of Research Impact
Authors: Swacha Jakub
Published: June 2022

The number of received citations and more complex bibliographic measures calculated based on them, such as the h-index, remain the most widely used indicators for measuring research impact in an objective and easy-to-compute way. However, using the number of received citations as a research impact measure has its shortcomings, some intrinsic (stemming from the doubt whether a citation is actually a confirmation of the cited paper’s impact), some extrinsic (stemming from the ease of manipulating this measure by deliberately inserting multiple unmerited references). While the first can only be addressed by a careful interpretation of the measure with consideration of its limitations, the latter can be reduced to much extent by replacing simple citation counting with a more sophisticated procedure constraining the impact that a single publication may have on the others. One such solution is ArticleRank, which has, however, several disadvantages limiting its practical use. In this paper, we propose another solution to this problem, the Transitive Research Impact Score (TRIS), which is free of these disadvantages, and validate it on a sample dataset.
Deformed Mediated Larval Incisor Lobe Development Causes Differing Feeding Behavior between Oriental Armyworm and Fall Armyworm
Authors: Zhao Hailong , Zhu Zeng , Xing Gaoliang , Li Yiyu , Zhou Xue , Wang Jingjing , Li Guiting , Cao Haiqun , Huang Yong
Published: June 2022

Mandibular incisor lobes are important for insect feeding behavior, living habits and niche. However, the molecular regulation of insect incisor lobe development remains unknown. In this study, we found that two maize pests, oriental armyworm Mythimna separata and fall armyworm Spodoptera frugiperda, have different feeding patterns in maize, which are closely associated with the different development patterns of their incisor lobes. Different from first to sixth instar S. frugiperda, which feed on leaf tissues and whorls with sharp incisor lobes, older instars of M. separata feed from leaf margins with no incisor lobes. Hox gene Deformed (Dfd) is important for head appendages, but its function in incisor lobe development is not clear. Here, Dfds were identified from two armyworm species, and both were expressed highly in heads and eggs. Interestingly, the expression levels of MsDfd were relatively high in larval mandibles and decreased dramatically from fourth-instar mandibles in M. separata. Knockdown of MsDfd resulted in malformed mandibles with no incisor lobe in M. separata, making the larvae unable to perform window-feeding. However, RNAi of SfDfd did not affect the mandibles and window-feeding pattern of S. frugiperda, indicating the different roles of Dfd in these two species. Moreover, the mortality of new first instar M. separata increased after feeding dsMsDfd but did not for S. frugiperda feeding dsSfDfd. These findings revealed that Dfd mediated the larval mandibular incisor lobe morphology, affecting its feeding pattern in M. separata, broadening the knowledge of Dfd functions in insect mandibles and feeding behavior.
A Waveform Mapping-Based Approach for Enhancement of Trunk Borers’ Vibration Signals Using Deep Learning Model
Authors: Shi Haopeng , Chen Zhibo , Zhang Haiyan , Li Juhu , Liu Xuanxin , Ren Lili , Luo Youqing
Published: June 2022

The larvae of some trunk-boring beetles barely leave traces on the outside of trunks when feeding within, rendering the detection of them rather difficult. One approach to solving this problem involves the use of a probe to pick up boring vibrations inside the trunk and distinguish larvae activity according to the vibrations. Clean boring vibration signals without noise are critical for accurate judgement. Unfortunately, these environments are filled with natural or artificial noise. To address this issue, we constructed a boring vibration enhancement model named VibDenoiser, which makes a significant contribution to this rarely studied domain. This model is built using the technology of deep learning-based speech enhancement. It consists of convolutional encoder and decoder layers with skip connections, and two layers of SRU++ for sequence modeling. The dataset constructed for study is made up of boring vibrations of Agrilus planipennis Fairmaire, 1888 (Coleoptera: Buprestidae) and environmental noise. Our VibDenoiser achieves an improvement of 18.57 in SNR, and it runs in real-time on a laptop CPU. The accuracy of the four classification models increased by a large margin using vibration clips enhanced by our model. The results demonstrate the great enhancement performance of our model, and the contribution of our work to better boring vibration detection.
Systematic Review of the Genus Nalepa Reitter, 1887 (Coleoptera, Tenebrionidae, Blaptinae, Blaptini) from the Tibetan Plateau, with Description of Six New Species and Two Larvae
Authors: Li Xiu-Min , Tian Juan , Fan Jiao-Jiao , Ren Guo-Dong
Published: June 2022

Nalepa Reitter, 1887 is a monotypic genus of the tenebrionid tribe Blaptini and is endemic to the Tibetan Plateau. In this study, the genus Nalepa was reviewed using a combination of molecular and morphological datasets. Based on the results, six new species were described: N.acuminata sp. n., N. ovalifolia sp. n., N.polita sp. n., N. quadrata sp. n., N.xinlongensis sp. n., and N.yushuensis sp. n. In addition, the larvae of N. cylindracea Reitter, 1887 and N. quadrata sp. n. were described and associated with related adults using molecular approaches. This study provides valuable molecular and morphological data for phylogenetic studies.
Improvement of Fault Current Calculation and Static Security Risk for Droop Control of the Inverter-Interfaced DG of Grid-Connected and Isolated Microgrids
Authors: Aref Mahmoud , Mossa Mahmoud A. , Lan Ngo Kim , Quynh Nguyen Vu , Oboskalov Vladislav , Ali Alaa F. M.
Published: June 2022

The contribution current of an inverter-interfaced distributed generator unit during a fault is one of the significant challenges for two modes: grid-connected and isolated AC microgrid. For this challenge, this article is aimed to study two methods of fault current calculation for two modes: grid-connected and isolated microgrids. These methods include a virtual equivalent impedance and a proposed method. The proposed method is a new technique for calculating the fault current contribution depending on the droop control of inverter-interfaced DG. The proposed method can control the contribution short-circuit current of DG within its limit (2 p.u.) where it is dependent on the voltage value of the DG bus to calculate the short circuit current of DG by using the control criterion. Static security risk and load shedding are calculated after fault clearance using an operation scenario in which the distribution system will be divided into small subsystems and is then grid-connected and isolated due to the removal of the faulted bus by protection devices. The proposed technique is applied to a standard IEEE 33-bus distribution network with five DGs. The results show that the contribution current of inverter-interfaced DG during the fault has more effects than the fault current of the nearest faulted bus to the DG bus. The proposed technique improves the calculated fault current value by about 30% for the grid-connected microgrid and by about 50% for the isolated microgrid from its value of the virtual impedance method. The static security risk is improved after load shedding. The static security risk improved by about 0.025%.