bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of Running Performance in the Offensive and Defensive Phases of the Game: Is It Associated with the Team Achievement in the UEFA Champions League?
Authors: Modric Toni , Versic Sime , Drid Patrik , Stojanovic Marko , Radzimiński Łukasz , Bossard Cyril , Aftański Tomasz , Sekulic Damir
Published: September 2021

Although associations between running performance (RP) with ball possession and team achievement in soccer are often hypothesized, actual knowledge of this association in elite soccer remains limited. Therefore, this study aimed to evaluate players’ RPs according to ball possession to determine its possible influence on team achievement in the UEFA Champions League (UCL). The players’ RPs (n = 244) were collected during UCL group stage matches (n = 20) in the 2020/21 season using the semiautomatic video system InStat Fitness. Then, players’ RPs were classified according to the specific playing position: central defenders (CD; n = 79), fullbacks (FB; n = 65), central midfielders (CM; n = 55), wide midfielders (WM; n = 28) and forwards (FW; n = 17). RPs were observed in the attacking phase (AP, i.e., when the team was in possession of the ball) and defensive phase (DP, i.e., when the team did not have possession of the ball) of the game, and included the total distance covered (m) and distance covered in different categories: walking (<7.1 km/h), jogging (7.1–14.3 km/h), running (14.4–19.7 km/h), high-intensity running (>19.8 km/h), high-speed running (19.8–25.1 km/h) and sprinting (>25.2 km/h). Team achievement was defined by the total group points earned (TGP) at the end of the group phase of the UCL and by match outcome (win, draw, loss) of single matches. The results indicated that the total, walking and jogging distances covered were negatively and positively associated with TGP (Pearson’s correlations from 0.30 to 0.73; all p < 0.05) in the AP and DP of the game, respectively. Won matches were characterized by significantly lower and higher values of total, walking and jogging distances covered in AP and DP of the game, respectively (F tests: from 7.15 to 22.5, all p < 0.01; all small to medium effect sizes). In addition, RPs in the AP and DP of the game explained only 37.2% of the variance in the TGP. These findings demonstrate that the influence of RP on team achievement in UCL is limited in both the AP and DP of the game.
Recycled Tire Rubber in Additive Manufacturing: Selective Laser Sintering for Polymer-Ground Rubber Composites
Authors: Toncheva Antoniya , Brison Loïc , Dubois Philippe , Laoutid Fouad
Published: September 2021

Natural and synthetic rubber is gaining increased interest in various industrial applications and daily life sectors (automotive industry, acoustic and electrical isolators, adhesives, impermeable surfaces, and others) due to its remarkable physicomechanical properties, excellent durability, and abrasive resistance. These great characteristics are accompanied by some recycling difficulties of the final products, particularly originated from the tire waste rubber industry. In this study, recycled tire rubber was incorporated in polymer matrices using selective laser sintering as 3D printing technology. Two polymers were used-polyamide and thermoplastic polyurethane, for their rigid and elastomeric properties, respectively. Polymer composites containing various tire powder amounts, up to 40 wt.%, were prepared by physical blending. The final materials’ morphological characteristics, mechanical properties, and thermal stability were evaluated. The proposed ambitious additive manufacturing approach looked over also some of the major aspects to be considered during the 3D printing procedure. In addition, examples of printed prototypes with potential applications were also proposed revealing the potential of the recycled tire rubber-loaded composites.
First Dye-Decolorizing Peroxidase from an Ascomycetous Fungus Secreted by Xylaria grammica
Authors: Kimani Virginia , Ullrich René , Büttner Enrico , Herzog Robert , Kellner Harald , Jehmlich Nico , Hofrichter Martin , Liers Christiane
Published: September 2021

Background: Fungal DyP-type peroxidases have so far been described exclusively for basidiomycetes. Moreover, peroxidases from ascomycetes that oxidize Mn2+ ions are yet not known. Methods: We describe here the physicochemical, biocatalytic, and molecular characterization of a DyP-type peroxidase (DyP, EC 1.11.1.19) from an ascomycetous fungus. Results: The enzyme oxidizes classic peroxidase substrates such as 2,6-DMP but also veratryl alcohol and notably Mn2+ to Mn3+ ions, suggesting a physiological function of this DyP in lignin modification. The KM value (49 µM) indicates that Mn2+ ions bind with high affinity to the XgrDyP protein but their subsequent oxidation into reactive Mn3+ proceeds with moderate efficiency compared to MnPs and VPs. Mn2+ oxidation was most effective at an acidic pH (between 4.0 and 5.0) and a hypothetical surface exposed an Mn2+ binding site comprising three acidic amino acids (two aspartates and one glutamate) could be localized within the hypothetical XgrDyP structure. The oxidation of Mn2+ ions is seemingly supported by four aromatic amino acids that mediate an electron transfer from the surface to the heme center. Conclusions: Our findings shed new light on the possible involvement of DyP-type peroxidases in lignocellulose degradation, especially by fungi that lack prototypical ligninolytic class II peroxidases.
Peripheral Nerve Impairment in a Mouse Model of Alzheimer’s Disease
Authors: Torcinaro Alessio , Ricci Valentina , Strimpakos Georgios , De Santa Francesca , Middei Silvia
Published: September 2021

Sarcopenia, a geriatric syndrome involving loss of muscle mass and strength, is often associated with the early phases of Alzheimer’s disease (AD). Pathological hallmarks of AD including amyloid β (Aβ) aggregates which can be found in peripheral tissues such as skeletal muscle. However, not much is currently known about their possible involvement in sarcopenia. We investigated neuronal innervation in skeletal muscle of Tg2576 mice, a genetic model for Aβ accumulation. We examined cholinergic innervation of skeletal muscle in adult Tg2576 and wild type mice by immunofluorescence labeling of tibialis anterior (TA) muscle sections using antibodies raised against neurofilament light chain (NFL) and acetylcholine (ACh) synthesizing enzyme choline acetyltransferase (ChAT). Combining this histological approach with real time quantification of mRNA levels of nicotinic acetylcholine receptors, we demonstrated that in the TA of Tg2576 mice, neuronal innervation is significantly reduced and synaptic area is smaller and displays less ChAT content when compared to wild type mice. Our study provides the first evidence of reduced cholinergic innervation of skeletal muscle in a mouse model of Aβ accumulation. This evidence sustains the possibility that sarcopenia in AD originates from Aβ-mediated cholinergic loss.
The Mechanisms Underlying Interference and Inhibition: A Review of Current Behavioral and Neuroimaging Research
Authors: Kliegl Oliver , Bäuml Karl-Heinz T.
Published: September 2021

The memory literature has identified interference and inhibition as two major sources of forgetting. While interference is generally considered to be a passive cause of forgetting arising from exposure to additional information that impedes subsequent recall of target information, inhibition concerns a more active and goal-directed cause of forgetting that can be achieved intentionally. Over the past 25 years, our knowledge of the neural mechanisms underlying both interference-induced and inhibition-induced forgetting has expanded substantially. The present paper gives a critical overview of this research, pointing out empirical gaps in the current work and providing suggestions for future studies.
The Impact of Wildfires on Mental Health: A Scoping Review
Authors: To Patricia , Eboreime Ejemai , Agyapong Vincent I. O.
Published: September 2021

One of the many consequences of climate change is an increase in the frequency, severity, and, thus, impact of wildfires across the globe. The destruction and loss of one’s home, belongings, and surrounding community, and the threat to personal safety and the safety of loved ones can have significant consequences on survivors’ mental health, which persist for years after. The objective of this scoping review was to identify primary studies examining the impact of wildfires on mental health and to summarize findings for PTSD, depression, anxiety, and substance use. Literature searches on Pubmed and Embase were conducted in February and April of 2021, respectively, with no date restrictions. A total of 254 studies were found in the two database searches, with 60 studies meeting the inclusion criteria. Three other studies were identified and included based on relevant in-text citations during data abstraction. The results show an increased rate of PTSD, depression, and generalized anxiety at several times of follow-up post-wildfire, from the subacute phase, to years after. An increased rate of mental health disorders post-wildfire has been found in both the adult and pediatric population, with a number of associated risk factors, the most significant being characteristics of the wildfire trauma itself. Several new terms have arisen in the literature secondary to an increased awareness and understanding of the impact of natural disasters on mental health, including ecological grief, solastalgia, and eco-anxiety. There are a number of patient factors and systemic changes that have been identified post-wildfire that can contribute to resilience and recovery.
Utilization of Corn Cob Ash as Fine Aggregate and Ground Granulated Blast Furnace Slag as Cementitious Material in Concrete
Authors: Bheel Naraindas , Ali Montasir Osman Ahmed , Liu Yue , Tafsirojjaman T. , Awoyera Paul , Sor Nadhim Hamah , Bendezu Romero Lenin Miguel
Published: September 2021

Cementitious and recycled materials that have the potential to improve various properties of concrete have attracted the attention of many researchers recently. Different types of cementitious and recycled materials seem to possess certain unique properties to change cement concrete. This experimental study aims to investigate the impact of ground granulated blast furnace slag (GGBFS) and corn cob ash (CCA) as a partial replacement material for Portland cement (PC) and fine aggregate (FA), respectively, on fresh and hardened concrete properties, as well as the embodied carbon of concrete. The concrete mix was blended with 5–20% of GGBFS and 10–40% of corn cob ash, both individually and combined. A total of 300 concrete specimens were made to achieve the targeted strength of 25 MPa at a 0.50 water/cement ratio and cured at 28 days. It is observed that the workability of fresh concrete is lowered as the dosages of GGBFS and CCA increase in the mixture. Moreover, the compressive and split tensile strengths are augmented by 10.94% and 9.15%, respectively, at 10% of GGBFS by the weight of PC at 28 days. Similarly, the compressive and split tensile strengths are augmented by 11.62% and 10.56%, respectively, at 30% of CCA by the weight of FA at 28 days. Moreover, the combined use of 10% of GGBFS as a cementitious ingredient along with 30% of fine aggregate replaced with CCA in concrete provides the highest compressive and splitting tensile strength, with 16.98% and 13.38% at 28 days, respectively. Furthermore, the density and water absorption of concrete were reduced with increasing dosages of GGBFS and FA in concrete at 28 days. In addition, the embodied carbon and energy were also reduced as the replacement content of GGBFS along with CCA increased in concrete. It is concluded that 10% of GGBFS and 30% of CCA are the optimum percentages for structural applications to reduce the use of cement as well as the cost of the project.
Covalent Organic Frameworks for Simultaneous CO2 Capture and Selective Catalytic Transformation
Authors: Li Yaling , Zhang Jianqiang , Zuo Kaiming , Li Zhongping , Wang Yu , Hu Hui , Zeng Chaoyuan , Xu Huanjun , Wang Baoshan , Gao Yanan
Published: September 2021

Combination of capture and simultaneous conversion of CO2 into valuable chemicals is a fascinating strategy for reducing CO2 emissions. Therefore, searching for heterogeneous catalysts for efficient catalytic conversion of CO2 is of great importance for carbon capture and utilization. Herein, we report a metalloporphyrin-based covalent organic framework (Co(II)@TA-TF COF) that can capture CO2 and simultaneously convert it into cyclic carbonates under mild conditions. The COF was designed to possess micropores for the adsorption of CO2 and integrated with cobalt(II) porphyrin (Co(II)@TAPP) units as catalytic sites into the vertices of the layered tetragonal networks. The structure of the Co(II)@TA-TF COF is unique where Co(II)@TAPP units are alternately stacked along the z direction with a slipped distance of 1.7 Å, which gives an accessible space to accommodate small molecules, making it possible to expose catalytic sites to substrates within the adjacent stacked layers. As a result, this COF is found to be highly effective for the addition of CO2 and epoxides. Importantly, the Co(II)@TA-TF COF exhibited a dramatic size selectivity for substrates. In conjunction with its reusability, our results highlight the development of a new function of COFs for targeting simultaneous CO2 absorption and utilization upon complementary exploration of the structural features of skeletons and pores. Such promising catalytic performance of the COF makes it possible for its potential practical application.
Statins Decrease Programmed Death-Ligand 1 (PD-L1) by Inhibiting AKT and β-Catenin Signaling
Authors: Lim Woo-Jin , Lee Mingyu , Oh Yerin , Fang Xue-Quan , Lee Sujin , Lim Chang-Hoon , Park Jooho , Lim Ji-Hong
Published: September 2021

Retrospective observational studies have reported that statins improve clinical outcomes in patients previously treated with programmed cell death protein 1 (PD-1)-targeting monoclonal antibodies for malignant pleural mesothelioma (MPM) and advanced non-small cell lung cancer (NSCLC). In multiple mouse cancer models, de novo synthesis of mevalonate and cholesterol inhibitors was found to synergize with anti-PD-1 antibody therapy. In the present study, we investigated whether statins affect programmed death-ligand 1 (PD-L1) expression in cancer cells. Four statins, namely simvastatin, atorvastatin, lovastatin, and fluvastatin, decreased PD-L1 expression in melanoma and lung cancer cells. In addition, we found that AKT and β-catenin signaling involved PD-L1 suppression by statins. Our cellular and molecular studies provide inspiring evidence for extending the clinical evaluation of statins for use in combination with immune checkpoint inhibitor-based cancer therapy.
Neuroprotective Effects and Therapeutic Potential of Transcorneal Electrical Stimulation for Depression
Authors: Yu Wing-Shan , Kwon So-Hyun , Agadagba Stephen Kugbere , Chan Leanne-Lai-Hang , Wong Kah-Hui , Lim Lee-Wei
Published: September 2021

Transcorneal electrical stimulation (TES) has emerged as a non-invasive neuromodulation approach that exerts neuroprotection via diverse mechanisms, including neurotrophic, neuroplastic, anti-inflammatory, anti-apoptotic, anti-glutamatergic, and vasodilation mechanisms. Although current studies of TES have mainly focused on its applications in ophthalmology, several lines of evidence point towards its putative use in treating depression. Apart from stimulating visual-related structures and promoting visual restoration, TES has also been shown to activate brain regions that are involved in mood alterations and can induce antidepressant-like behaviour in animals. The beneficial effects of TES in depression were further supported by its shared mechanisms with FDA-approved antidepressant treatments, including its neuroprotective properties against apoptosis and inflammation, and its ability to enhance the neurotrophic expression. This article critically reviews the current findings on the neuroprotective effects of TES and provides evidence to support our hypothesis that TES possesses antidepressant effects.
Metabolomic Fingerprint of Mecp2-Deficient Mouse Cortex: Evidence for a Pronounced Multi-Facetted Metabolic Component in Rett Syndrome
Authors: Golubiani Gocha , Lagani Vincenzo , Solomonia Revaz , Müller Michael
Published: September 2021

Using unsupervised metabolomics, we defined the complex metabolic conditions in the cortex of a mouse model of Rett syndrome (RTT). RTT, which represents a cause of mental and cognitive disabilities in females, results in profound cognitive impairment with autistic features, motor disabilities, seizures, gastrointestinal problems, and cardiorespiratory irregularities. Typical RTT originates from mutations in the X-chromosomal methyl-CpG-binding-protein-2 (Mecp2) gene, which encodes a transcriptional modulator. It then causes a deregulation of several target genes and metabolic alterations in the nervous system and peripheral organs. We identified 101 significantly deregulated metabolites in the Mecp2-deficient cortex of adult male mice; 68 were increased and 33 were decreased compared to wildtypes. Pathway analysis identified 31 mostly upregulated metabolic pathways, in particular carbohydrate and amino acid metabolism, key metabolic mitochondrial/extramitochondrial pathways, and lipid metabolism. In contrast, neurotransmitter-signaling is dampened. This metabolic fingerprint of the Mecp2-deficient cortex of severely symptomatic mice provides further mechanistic insights into the complex RTT pathogenesis. The deregulated pathways that were identified—in particular the markedly affected amino acid and carbohydrate metabolism—confirm a complex and multifaceted metabolic component in RTT, which in turn signifies putative therapeutic targets. Furthermore, the deregulated key metabolites provide a choice of potential biomarkers for a more detailed rating of disease severity and disease progression.
Isolation and Characterization of Tissue Resident CD29-Positive Progenitor Cells in Livestock to Generate a Three-Dimensional Meat Bud
Authors: Naraoka Yuna , Mabuchi Yo , Yoneyama Yosuke , Suto Eriko Grace , Hisamatsu Daisuke , Ikeda Mami , Ito Risa , Nakamura Tetsuya , Takebe Takanori , Akazawa Chihiro
Published: September 2021

The current process of meat production using livestock has significant effects on the global environment, including high emissions of greenhouse gases. In recent years, cultured meat has attracted attention as a way to acquire animal proteins. However, the lack of markers that isolate proliferating cells from bovine tissues and the complex structure of the meat make it difficult to culture meat in a dish. In this study, we screened 246 cell-surface antibodies by fluorescence-activated cell sorting for their capacity to form colonies and their suitability to construct spheroid “meat buds”. CD29+ cells (Ha2/5 clone) have a high potency to form colonies and efficiently proliferate on fibronectin-coated dishes. Furthermore, the meat buds created from CD29+ cells could differentiate into muscle and adipose cells in a three-dimensional structure. The meat buds embedded in the collagen gel proliferated in the matrix and formed large aggregates. Approximately 10 trillion cells can theoretically be obtained from 100 g of bovine tissue by culturing and amplifying them using these methods. The CD29+ cell characteristics of bovine tissue provide insights into the production of meat alternatives in vitro.
Are Interactions between Epicardial Adipose Tissue, Cardiac Fibroblasts and Cardiac Myocytes Instrumental in Atrial Fibrosis and Atrial Fibrillation?
Authors: Krishnan Anirudh , Chilton Emily , Raman Jaishankar , Saxena Pankaj , McFarlane Craig , Trollope Alexandra F. , Kinobe Robert , Chilton Lisa
Published: September 2021

Atrial fibrillation is very common among the elderly and/or obese. While myocardial fibrosis is associated with atrial fibrillation, the exact mechanisms within atrial myocytes and surrounding non-myocytes are not fully understood. This review considers the potential roles of myocardial fibroblasts and myofibroblasts in fibrosis and modulating myocyte electrophysiology through electrotonic interactions. Coupling with (myo)fibroblasts in vitro and in silico prolonged myocyte action potential duration and caused resting depolarization; an optogenetic study has verified in vivo that fibroblasts depolarized when coupled myocytes produced action potentials. This review also introduces another non-myocyte which may modulate both myocardial (myo)fibroblasts and myocytes: epicardial adipose tissue. Epicardial adipocytes are in intimate contact with myocytes and (myo)fibroblasts and may infiltrate the myocardium. Adipocytes secrete numerous adipokines which modulate (myo)fibroblast and myocyte physiology. These adipokines are protective in healthy hearts, preventing inflammation and fibrosis. However, adipokines secreted from adipocytes may switch to pro-inflammatory and pro-fibrotic, associated with reactive oxygen species generation. Pro-fibrotic adipokines stimulate myofibroblast differentiation, causing pronounced fibrosis in the epicardial adipose tissue and the myocardium. Adipose tissue also influences myocyte electrophysiology, via the adipokines and/or through electrotonic interactions. Deeper understanding of the interactions between myocytes and non-myocytes is important to understand and manage atrial fibrillation.
Usefulness of Endoscopic Ultrasound with the Jelly-Filling Method for Esophageal Varices
Authors: Kato Tsunetaka , Hikichi Takuto , Nakamura Jun , Takasumi Mika , Hashimoto Minami , Kobashi Ryoichiro , Yanagita Takumi , Takagi Tadayuki , Suzuki Rei , Sugimoto Mitsuru , Sato Yuki , Irie Hiroki , Okubo Yoshinori , Kobayakawa Masao , Ohira Hiromasa
Published: September 2021

Although the importance of endoscopic ultrasound (EUS) for esophageal varices (EVs) has been demonstrated, it is difficult to obtain sufficient EUS images with the water-filling method because of poor water stagnation in the esophagus. In this study on EVs, we aimed to evaluate the usefulness of the jelly-filling method for EUS. Consecutive patients who underwent EUS for EVs were included. The quality of EUS images, the diagnostic ability of the presence of blood vessels inside and outside the esophageal wall, and the procedure time were compared between the jelly-filling and water-filling methods. Thirty cases were analyzed (jelly-filling method in 13 and water-filling method in 17). The EUS image quality score was significantly higher in the jelly-filling method (jelly vs. water; three points vs. two points, p < 0.001). Additionally, EUS image quality scores in both nonexperts and experts were significantly higher in the jelly-filling method. The diagnostic ability of the presence of perforation veins was significantly higher in the jelly-filling method (jelly vs. water; 100% vs. 52.9%, p = 0.004). However, the procedure time was significantly longer in the jelly-filling method (p = 0.024). In conclusion, EUS using the jelly-filling method for EVs provided sufficient image quality.
Hybrid Additive Fabrication of a Transparent Liver with Biosimilar Haptic Response for Preoperative Planning
Authors: Aseni Paolo , Santaniello Tommaso , Rizzetto Francesco , Gentili Lorenzo , Pezzotta Federico , Cavaliere Francesco , Vertemati Maurizio , Milani Paolo
Published: September 2021

Due to the complexity of liver surgery, the interest in 3D printing is constantly increasing among hepatobiliary surgeons. The aim of this study was to produce a patient-specific transparent life-sized liver model with tissue-like haptic properties by combining additive manufacturing and 3D moulding. A multistep pipeline was adopted to obtain accurate 3D printable models. Semiautomatic segmentation and registration of routine medical imaging using 3D Slicer software allowed to obtain digital objects representing the structures of interest (liver parenchyma, vasculo-biliary branching, and intrahepatic lesion). The virtual models were used as the source data for a hybrid fabrication process based on additive manufacturing using soft resins and casting of tissue-mimicking silicone-based blend into 3D moulds. The model of the haptic liver reproduced with high fidelity the vasculo-biliary branching and the relationship with the intrahepatic lesion embedded into the transparent parenchyma. It offered high-quality haptic perception and a remarkable degree of surgical and anatomical information. Our 3D transparent model with haptic properties can help surgeons understand the spatial changes of intrahepatic structures during surgical manoeuvres, optimising preoperative surgical planning.
Factor Analysis of Well Logs for Total Organic Carbon Estimation in Unconventional Reservoirs
Authors: Szabó Norbert P. , Valadez-Vergara Rafael , Tapdigli Sabuhi , Ugochukwu Aja , Szabó István , Dobróka Mihály
Published: September 2021

Several approaches have been applied for the evaluation of formation organic content. For further developments in the interpretation of organic richness, this research proposes a multivariate statistical method for exploring the interdependencies between the well logs and model parameters. A factor analysis-based approach is presented for the quantitative determination of total organic content of shale formations. Uncorrelated factors are extracted from well logging data using Jöreskog’s algorithm, and then the factor logs are correlated with estimated petrophysical properties. Whereas the first factor holds information on the amount of shaliness, the second is identified as an organic factor. The estimation method is applied both to synthetic and real datasets from different reservoir types and geologic basins, i.e., Derecske Trough in East Hungary (tight gas); Kingak formation in North Slope Alaska, United States of America (shale gas); and shale source rock formations in the Norwegian continental shelf. The estimated total organic content logs are verified by core data and/or results from other indirect estimation methods such as interval inversion, artificial neural networks and cluster analysis. The presented statistical method used for the interpretation of wireline logs offers an effective tool for the evaluation of organic matter content in unconventional reservoirs.
Synthesis and Characterization of ZnO from Thermal Decomposition of Precipitated Zinc Oxalate Dihydrate as an Anode Material of Li-Ion Batteries
Authors: Yudha Cornelius Satria , Hutama Anjas Prasetya , Rahmawati Mintarsih , Widiyandari Hendri , Nursukatmo Hartoto , Nilasary Hanida , Oktaviano Haryo Satriya , Purwanto Agus
Published: September 2021

Zinc oxide (ZnO) is one of the most promising materials applied in Li-ion batteries. In this research, ZnO was synthesized by the thermal decomposition of zinc oxalate dihydrate. This precursor was obtained from the precipitation process of zinc sulfate with oxalic acid. In-depth studies were carried out on the effect of various heating temperatures of zinc oxalate dihydrate precursors on ZnO synthesis. The as-prepared materials were characterized by XRD, SEM, and FTIR. Based on the XRD analysis, the presence of the ZnO-wurtzite phase can be confirmed in samples heated at temperatures above 400 °C. Meanwhile, SEM-EDX results showed that the ZnO particles have a micron size. Cells with ZnO samples as anodes have low columbic efficiency. In contrast, cells with ZnO/Graphite composite anodes have a relatively large capacity compared to pure graphite anodes. Overall, based on the consideration of the characterization results and electrochemical performance, the optimal sintering temperature to obtain ZnO is 600 °C with a cell discharge capacity of ZnO anode and in the form of graphite composites is 356 mAh/g and 450 mAh/g, respectively. This suggests that ZnO can be used as an anode material and an additive component to improve commercial graphite anodes’ electrochemical performance.
Strengthening Gender Justice in a Just Transition: A Research Agenda Based on a Systematic Map of Gender in Coal Transitions
Authors: Walk Paula , Braunger Isabell , Semb Josephine , Brodtmann Carolin , Oei Pao-Yu , Kemfert Claudia
Published: September 2021

For climate change mitigation, a rapid phase-out of fossil fuels such as coal is necessary. This has far-reaching gender-specific consequences. This paper presents a systematic map of the literature that examines the impact of historical coal phase-out processes on women and their role in these processes. The search process consisted of screening over 3100 abstracts and reading 247 full-text studies. The analysis of the 73 publications ultimately included in the systematic map shows that past coal phase-outs meant both opportunities (e.g., increased labour market participation) as well as burdens for women (e.g., double burden of job and household). It becomes clear that agency within coal transitions was also gendered. For example, it was difficult for women to gain access to union structures, which led them to organise themselves into grassroots movements. Our research shows that policies aiming for a just sustainability transition should always be explicitly gender-responsive. However, the impact of sustainability transitions on women’s lives remains largely under-researched. Therefore, we propose a research agenda based on our findings containing six key issues that need to be addressed scientifically.
Advanced Metering Infrastructure—Towards a Reliable Network
Authors: Kornatka Mirosław , Popławski Tomasz
Published: September 2021

In order to ensure continuous energy supply, Distribution System Operators (DSOs) have to monitor and analyze the condition of the power grid, especially checking for random events, such as breakdowns or other disturbances. Still, relatively little information is available on the operation of the Low Voltage (LV) grid. This can be improved thanks to digital tools, offering online processing of data, which ultimately increases effectiveness of the power grid. Among those tools, the use of the Advanced Metering Infrastructure (AMI) is especially conducive for improving reliability. AMI is one of the elements of the system Supervisory Control and Data Acquisition (SCADA) for the LV grid. Exact knowledge of the reliability conditions of a power grid is also indispensable for optimizing investment. AMI is also key in providing operational capacity for carrying out energy balance in virtual power plants (VPPs). This paper deals with methodology of identification and location of faults in the AMI-supervised LV grid and with calculating the System Average Interruption Duration Index (SAIDI) and System Average Interruption Frequency Index (SAIFI) on the basis of the recorded events. The results presented in the paper are based on data obtained from seven MV/LV transformer stations that supply over 2000 customers.
The Development and Application of a GIS-Based Tool to Assess Forest Landscape Restoration Effects on Water Conservation Capacity
Authors: Yu Enxu , Zhang Mingfang , Xu Yali , Zhang Sheng , Meng Zuozhu , Hou Yiping
Published: September 2021

In forest landscape restoration, one of the key objectives is to improve the water conservation capacity of the deforested land. A rapid, accurate assessment of the effects of the restoration measures on the water conservation capacity of targeted forests can help forest managers to identify the best practices for forest restoration. However, the traditional assessment tools of forest water conservation function lack a description of forest growth, and are featured by complex computation, which fails to evaluate the effects of forest restoration on the regional forest water conservation capacity in an efficient way. To address this issue, through combining the forest restoration evaluation model (equivalent recovery area, ERA), classic forest water storage capacity estimation (total water storage capacity), this study has taken advantage of ENVI/IDL, ArcGIS Engine/C#.Net to develop the Forest and Water Assessment Tool (FWAT) for assessing the changes of the regional forest landscape and the associated forest water conservation capacity in various forest restoration scenarios. This tool has been successfully applied in the Upper Zagunao watershed, a large forested watershed in the Upper Yangtze River basin. According to the assessment, the forest water conservation capacity of the study watershed consistently increased from about 1580.76 t/hm2 in 2010 to a projected 2014.34 t/hm2 by natural restoration, and 2124.18 t/hm2 by artificial restoration by 2030. The artificial restoration measures yield a better effect on forest water conservation function than natural restoration. By 2030, the forest water conservation capacity of artificial restoration scenario is expected to be about 7% higher than that of natural restoration scenario. The FWAT as an efficient tool to assess the effects of forest restoration measures on regional forest water conservation capacity can provide scientific support for the design of forest restoration and management strategies worldwide.
Whole-Genome Profiles of Malay Colorectal Cancer Patients with Intact MMR Proteins
Authors: Juhari Wan Khairunnisa Wan , Ahmad Amin Noordin Khairul Bariah , Zakaria Andee Dzulkarnaen , Rahman Wan Faiziah Wan Abdul , Mokhter Wan Muhamad Mokhzani Wan Muhamad , Hassan Muhammad Radzi Abu , Sidek Ahmad Shanwani Mohammed , Zilfalil Bin Alwi
Published: September 2021

Background: This study aimed to identify new genes associated with CRC in patients with normal mismatch repair (MMR) protein expression. Method: Whole-genome sequencing (WGS) was performed in seven early-age-onset Malay CRC patients. Potential germline genetic variants, including single-nucleotide variations and insertions and deletions (indels), were prioritized using functional and predictive algorithms. Results: An average of 3.2 million single-nucleotide variations (SNVs) and over 800 indels were identified. Three potential candidate variants in three genes—IFNE, PTCH2 and SEMA3D—which were predicted to affect protein function, were identified in three Malay CRC patients. In addition, 19 candidate genes—ANKDD1B, CENPM, CLDN5, MAGEB16, MAP3K14, MOB3C, MS4A12, MUC19, OR2L8, OR51Q1, OR51AR1, PDE4DIP, PKD1L3, PRIM2, PRM3, SEC22B, TPTE, USP29 and ZNF117—harbouring nonsense variants were prioritised. These genes are suggested to play a role in cancer predisposition and to be associated with cancer risk. Pathway enrichment analysis indicated significant enrichment in the olfactory signalling pathway. Conclusion: This study provides a new spectrum of insights into the potential genes, variants and pathways associated with CRC in Malay patients.
CpBBX19, a B-Box Transcription Factor Gene of Chimonanthus praecox, Improves Salt and Drought Tolerance in Arabidopsis
Authors: Wu Huafeng , Wang Xia , Cao Yinzhu , Zhang Haiyuan , Hua Run , Liu Huamin , Sui Shunzhao
Published: September 2021

Zinc-finger proteins are important transcription factors in plants, responding to adversity and regulating the growth and development of plants. However, the roles of the BBX gene family of zinc-finger proteins in wintersweet (Chimonanthus praecox) have yet to be elucidated. In this study, a group IV subfamily BBX gene, CpBBX19, was identified and isolated from wintersweet. Quantitative real-time PCR (qRT-PCR) analyses revealed that CpBBX19 was expressed in all tissues and that expression was highest in cotyledons and inner petals. CpBBX19 was also expressed in all flower development stages, with the highest expression detected in early initiating bloom, followed by late initiating bloom and bloom. In addition, the expression of CpBBX19 was induced by different abiotic stress (cold, heat, NaCl, and drought) and hormone (ABA and MeJA) treatments. Heterologous expression of CpBBX19 in Arabidopsis thaliana (Arabidopsis) enhanced the tolerance of this plant to salt and drought stress as electrolyte leakage and malondialdehyde (MDA) concentrations in transgenic Arabidopsis after stress treatments were significantly lower than those in wild-type (WT) plants. In conclusion, this research demonstrated that CpBBX19 plays a role in the abiotic stress tolerance of wintersweet. These findings lay a foundation for future studies on the BBX gene family of wintersweet and enrich understanding of the molecular mechanism of stress resistance in wintersweet.
Enjoy Carefully: The Multifaceted Role of Vitamin E in Neuro-Nutrition
Authors: Regner-Nelke Liesa , Nelke Christopher , Schroeter Christina B. , Dziewas Rainer , Warnecke Tobias , Ruck Tobias , Meuth Sven G.
Published: September 2021

Vitamin E is often associated with health benefits, such as antioxidant, anti-inflammatory and cholesterol-lowering effects. These properties make its supplementation a suitable therapeutic approach in neurodegenerative disorders, for example, Alzheimer’s or Parkinson’s disease. However, trials evaluating the effects of vitamin E supplementation are inconsistent. In randomized controlled trials, the observed associations often cannot be substantiated. This could be due to the wide variety of study designs regarding the dosage and duration of vitamin E supplementation. Furthermore, genetic variants can influence vitamin E uptake and/or metabolism, thereby distorting its overall effect. Recent studies also show adverse effects of vitamin E supplementation regarding Alzheimer’s disease due to the increased synthesis of amyloid β. These diverse effects may underline the inhomogeneous outcomes associated with its supplementation and argue for a more thoughtful usage of vitamin E. Specifically, the genetic and nutritional profile should be taken into consideration to identify suitable candidates who will benefit from supplementation. In this review, we will provide an overview of the current knowledge of vitamin E supplementation in neurodegenerative disease and give an outlook on individualized, sustainable neuro-nutrition, with a focus on vitamin E supplementation.
Association of miR-499 Polymorphism and Its Regulatory Networks with Hashimoto Thyroiditis Susceptibility: A Population-Based Case-Control Study
Authors: Tabasi Farhad , Hasanpour Vahed , Sarhadi Shamim , Kaykhaei Mahmoud Ali , Pourzand Pouria , Heravi Mehrdad , Langari Ahmad Alinaghi , Bahari Gholamreza , Taheri Mohsen , Hashemi Mohammad , Ghavami Saeid
Published: September 2021

Hashimoto thyroiditis (HT) is a common autoimmune disorder with a strong genetic background. Several genetic factors have been suggested, yet numerous genetic contributors remain to be fully understood in HT pathogenesis. MicroRNAs (miRs) are gene expression regulators critically involved in biological processes, of which polymorphisms can alter their function, leading to pathologic conditions, including autoimmune diseases. We examined whether miR-499 rs3746444 polymorphism is associated with susceptibility to HT in an Iranian subpopulation. Furthermore, we investigated the potential interacting regulatory network of the miR-499. This case-control study included 150 HT patients and 152 healthy subjects. Genotyping of rs3746444 was performed by the PCR-RFLP method. Also, target genomic sites of the polymorphism were predicted using bioinformatics. Our results showed that miR-499 rs3746444 was positively associated with HT risk in heterozygous (OR = 3.32, 95%CI = 2.00–5.53, p < 0.001, CT vs. TT), homozygous (OR = 2.81, 95%CI = 1.30–6.10, p = 0.014, CC vs. TT), dominant (OR = 3.22, 95%CI = 1.97–5.25, p < 0.001, CT + CC vs. TT), overdominant (OR = 2.57, 95%CI = 1.62–4.09, p < 0.001, CC + TT vs. CT), and allelic (OR = 1.92, 95%CI = 1.37–2.69, p < 0.001, C vs. T) models. Mapping predicted target genes of miR-499 on tissue-specific-, co-expression-, and miR-TF networks indicated that main hub-driver nodes are implicated in regulating immune system functions, including immunorecognition and complement activity. We demonstrated that miR-499 rs3746444 is linked to HT susceptibility in our population. However, predicted regulatory networks revealed that this polymorphism is contributing to the regulation of immune system pathways.
Plasma Membrane Receptors Involved in the Binding and Response of Osteoclasts to Noncellular Components of the Bone
Authors: Karanth Divakar S. , Martin Macey L. , Holliday Lexie S.
Published: September 2021

Osteoclasts differentiate from hematopoietic cells and resorb the bone in response to various signals, some of which are received directly from noncellular elements of the bone. In vitro, adherence to the bone triggers the reduction of cell–cell fusion events between osteoclasts and the activation of osteoclasts to form unusual dynamic cytoskeletal and membrane structures that are required for degrading the bone. Integrins on the surface of osteoclasts are known to receive regulatory signals from the bone matrix. Regulation of the availability of these signals is accomplished by enzymatic alterations of the bone matrix by protease activity and phosphorylation/dephosphorylation events. Other membrane receptors are present in osteoclasts and may interact with as yet unidentified signals in the bone. Bone mineral has been shown to have regulatory effects on osteoclasts, and osteoclast activity is also directly modulated by mechanical stress. As understanding of how osteoclasts and other bone cells interact with the bone has emerged, increasingly sophisticated efforts have been made to create bone biomimetics that reproduce both the structural properties of the bone and the bone’s ability to regulate osteoclasts and other bone cells. A more complete understanding of the interactions between osteoclasts and the bone may lead to new strategies for the treatment of bone diseases and the production of bone biomimetics to repair defects.
Quest for the Molecular Basis of Improved Selective Toxicity of All-Trans Isomers of Aromatic Heptaene Macrolide Antifungal Antibiotics
Authors: Borzyszkowska-Bukowska Julia , Górska Justyna , Szczeblewski Paweł , Laskowski Tomasz , Gabriel Iwona , Jurasz Jakub , Kozłowska-Tylingo Katarzyna , Szweda Piotr , Milewski Sławomir
Published: September 2021

Three aromatic heptaene macrolide antifungal antibiotics, Candicidin D, Partricin A (Gedamycin) and Partricin B (Vacidin) were subjected to controlled cis-trans→ all trans photochemical isomerization. The obtained all-trans isomers demonstrated substantially improved in vitro selective toxicity in the Candida albicans cells: human erythrocytes model. This effect was mainly due to the diminished hemotoxicity. The molecular modeling studies on interactions between original antibiotics and their photoisomers with ergosterol and cholesterol revealed some difference in free energy profiles of formation of binary antibiotic/sterol complexes in respective membrane environments. Moreover, different geometries of heptaene: sterol complexes and variations in polyene macrolide molecule alignment in cholesterol-and ergosterol-containing membranes were found. None of these effects are of the crucial importance for the observed improvement of selective toxicity of aromatic heptaene antifungals but each seems to provide a partial contribution.
Genome-Wide Identification MIKC-Type MADS-Box Gene Family and Their Roles during Development of Floral Buds in Wheel Wingnut (Cyclocarya paliurus)
Authors: Qu Yinquan , Kong Weilong , Wang Qian , Fu Xiangxiang
Published: September 2021

MADS-box transcription factors (TFs) have fundamental roles in regulating floral organ formation and flowering time in flowering plants. In order to understand the function of MIKC-type MADS-box family genes in Cyclocarya paliurus (Batal.) Iljinskaja, we first implemented a genome-wide analysis of MIKC-type MADS-box genes in C. paliurus. Here, the phylogenetic relationships, chromosome location, conserved motif, gene structure, promoter region, and gene expression profile were analyzed. The results showed that 45 MIKC-type MADS-box were divided into 14 subfamilies: BS (3), AGL12 (1), AP3-PI (3), MIKC* (3), AGL15 (3), SVP (5), AGL17 (2), AG (3), TM8 (1), AGL6 (2), SEP (5), AP1-FUL (6), SOC1 (7), and FLC (1). The 43 MIKC-type MADS-box genes were distributed unevenly in 14 chromosomes, but two members were mapped on unanchored scaffolds. Gene structures were varied in the same gene family or subfamily, but conserved motifs shared similar distributions and sequences. The element analysis in promoters’ regions revealed that MIKC-type MADS-box family genes were associated with light, phytohormone, and temperature responsiveness, which may play important roles in floral development and differentiation. The expression profile showed that most MIKC-type MADS-box genes were differentially expressed in six tissues (specifically expressed in floral buds), and the expression patterns were also visibly varied in the same subfamily. CpaF1st24796 and CpaF1st23405, belonging to AP3-PI and SEP subfamilies, exhibited the high expression levels in PA-M and PG-F, respectively, indicating their functions in presenting heterodichogamy. We further verified the MIKC-type MADS-box gene expression levels on the basis of transcriptome and qRT-PCR analysis. This study would provide a theoretical basis for classification, cloning, and regulation of flowering mechanism of MIKC-type MADS-box genes in C. paliurus.
Exosomes as A Next-Generation Diagnostic and Therapeutic Tool in Prostate Cancer
Authors: Gaglani Simita , Gonzalez-Kozlova Edgar , Lundon Dara J. , Tewari Ashutosh K. , Dogra Navneet , Kyprianou Natasha
Published: September 2021

Extracellular vesicles (EVs) have brought great momentum to the non-invasive liquid biopsy procedure for the detection, characterization, and monitoring of cancer. Despite the common use of PSA (prostate-specific antigen) as a biomarker for prostate cancer, there is an unmet need for a more specific diagnostic tool to detect tumor progression and recurrence. Exosomes, which are EVs that are released from all cells, play a large role in physiology and pathology, including cancer. They are involved in intercellular communication, immune function, and they are present in every bodily fluid studied—making them an excellent window into how cells are operating. With liquid biopsy, EVs can be isolated and analyzed, enabling an insight into a potential therapeutic value, serving as a vehicle for drugs or nucleic acids that have anti-neoplastic effects. The current application of advanced technology also points to higher-sensitivity detection methods that are minimally invasive. In this review, we discuss the current understanding of the significance of exosomes in prostate cancer and the potential diagnostic value of these EVs in disease progression.
GABA: A Key Player in Drought Stress Resistance in Plants
Authors: Hasan Md. Mahadi , Alabdallah Nadiyah M. , Alharbi Basmah M. , Waseem Muhammad , Yao Guangqian , Liu Xu-Dong , Abd El-Gawad Hany G. , El-Yazied Ahmed Abou , Ibrahim Mohamed F. M. , Jahan Mohammad Shah , Fang Xiang-Wen
Published: September 2021

γ-aminobutyric acid (GABA) is a non-protein amino acid involved in various physiological processes; it aids in the protection of plants against abiotic stresses, such as drought, heavy metals, and salinity. GABA tends to have a protective effect against drought stress in plants by increasing osmolytes and leaf turgor and reducing oxidative damage via antioxidant regulation. Guard cell GABA production is essential, as it may provide the benefits of reducing stomatal opening and transpiration and controlling the release of tonoplast-localized anion transporter, thus resulting in increased water-use efficiency and drought tolerance. We summarized a number of scientific reports on the role and mechanism of GABA-induced drought tolerance in plants. We also discussed existing insights regarding GABA’s metabolic and signaling functions used to increase plant tolerance to drought stress.