bluuebunny/crossref_metadata_2025 huggingface dataset:
Retinal debris triggers cytotoxic damage in cocultivated primary porcine RPE cells
Authors: Wagner Natalie , Tsai Teresa , Reinehr Sabrina , Theile Janine , Dick H. Burkhard , Joachim Stephanie C.
Published: July 2024

Introduction

One of the most common causes of vision loss in the elderly population worldwide is age-related macular degeneration (AMD). Subsequently, the number of people affected by AMD is estimated to reach approximately 288 million by the year 2040. The aim of this study was to develop an ex vivo model that simulates various aspects of the complex AMD pathogenesis.

Methods

For this purpose, primary porcine retinal pigment epithelial cells (ppRPE) were isolated and cultured. One group was exposed to medium containing sodium iodate (NaIO3) to induce degeneration. The others were exposed to different supplemented media, such as bovine serum albumin (BSA), homogenized porcine retinas (HPR), or rod outer segments (ROOS) for eight days to promote retinal deposits. Then, these ppRPE cells were cocultured with porcine neuroretina explants for another eight days. To assess the viability of ppRPE cells, live/dead assay was performed at the end of the study. The positive RPE65 and ZO1 area was evaluated by immunocytochemistry and the expression of RLBP1, RPE65, and TJP1 was analyzed by RT-qPCR. Additionally, drusen (APOE), inflammation (ITGAM, IL6, IL8, NLRP3, TNF), oxidative stress (NFE2L2, SOD1, SOD2), and hypoxia (HIF1A) markers were investigated. The concentration of the inflammatory cytokines IL-6 and IL-8 was determined in medium supernatants from day 16 and 24 via ELISA.

Results

Live/dead assay suggests that especially exposure to NaIO3 and HPR induced damage to ppRPE cells, leading in a significant ppRPE cell loss. All supplemented media resulted in decreased RPE-characteristic markers (RPE65; ZO-1) and gene expression like RLBP1 and RPE65 in the cultured ppRPE cells. Besides, some inflammatory, oxidative as well as hypoxic stress markers were altered in ppRPE cells cultivated with NaIO3. The application of HPR induced an enhanced APOE expression. Pre-exposure of the ppRPE cells led to a diminished number of cones in all supplemented media groups compared to controls.

Discussion

Overall, this novel coculture model represents an interesting initial approach to incorporating deposits into coculture to mimic AMD pathogenesis. Nevertheless, the effects of the media used need to be investigated in further studies.
Wnt signaling pathway in spinal cord injury: from mechanisms to potential applications
Authors: Li Kai , Chen Zanzhi , Chang Xuejing , Xue Ruiyang , Wang Huaibo , Guo Weitao
Published: July 2024

Spinal cord injury (SCI) denotes damage to both the structure and function of the spinal cord, primarily manifesting as sensory and motor deficits caused by disruptions in neural transmission pathways, potentially culminating in irreversible paralysis. Its pathophysiological processes are complex, with numerous molecules and signaling pathways intricately involved. Notably, the pronounced upregulation of the Wnt signaling pathway post-SCI holds promise for neural regeneration and repair. Activation of the Wnt pathway plays a crucial role in neuronal differentiation, axonal regeneration, local neuroinflammatory responses, and cell apoptosis, highlighting its potential as a therapeutic target for treating SCI. However, excessive activation of the Wnt pathway can also lead to negative effects, highlighting the need for further investigation into its applicability and significance in SCI. This paper provides an overview of the latest research advancements in the Wnt signaling pathway in SCI, summarizing the recent progress in treatment strategies associated with the Wnt pathway and analyzing their advantages and disadvantages. Additionally, we offer insights into the clinical application of the Wnt signaling pathway in SCI, along with prospective avenues for future research direction.
Epidemiology and disease characteristics of myelofibrosis: a comparative analysis between Italy and global perspectives
Authors: Breccia Massimo , Palandri Francesca , Polverelli Nicola , Caira Morena , Berluti Michela , Palumbo Giuseppe A. , De Stefano Valerio
Published: July 2024

Myelofibrosis (MF) is a clonal disorder of hematopoietic stem cells characterized by altered bone marrow function and fibrosis. The aim of this narrative review is to report on the most recent epidemiologic data and to discuss features of MF and current strategies for the management of this condition in clinical practice. MF features covered by our review will include: characteristics of patients with MF; myeloproliferative and myelodepletive phenotypes; MF-associated thrombosis and bleeding; risk of infections; prefibrotic and overt PMF; secondary MF. Finally, we will discuss a few aspects of MF management in clinical practice and suggest strategies for its optimization and standardization. The focus of our paper is on Italy, but relevant data from other countries will also be reviewed.
Relationship between nausea and vomiting and physical activity in patients with lung cancer undergoing first chemotherapy
Authors: Bai Liandi , Ni Li , Lu JianHong , Zhang Yue Yu , Yin Yuanyuan , Zhang WeiYing , Duan Xia
Published: July 2024

Objective

Nausea and vomiting are the distressing and debilitating side effects of chemotherapy. This study explores the relationship between the degree of nausea and vomiting and physical activity in patients with lung cancer during the first chemotherapy cycle.

Design

A total of 107 patients with lung cancer who received platinum drugs during chemotherapy in a hospital in Shanghai, China, in 2023 were involved in this study. Data were collected with medical record system and self-reported questionnaires.Questionnaires included the International Physical Activity Questionnaire (IPAQ) and Index of Nausea, Vomiting, and Retching (INVR). IPAQ was used before chemotherapy; INVR was used on the second and sixth day of chemotherapy, followed by the analysis of the correlation between physical activity status and degree of nausea and vomiting during chemotherapy. The influencing factors of nausea and vomiting during chemotherapy in patients with lung cancer were analyzed using logistic regression analysis.

Results

More than half of the patients experienced nausea, vomiting or retching related symptoms after chemotherapy, and the proportion of moderate to high physical activity was 50.5%. Univariate analysis showed that the degree of nausea and vomiting was influenced by factors such as age, gender, and history of drinking (P < 0.05). The degree of nausea and vomiting was negatively correlated with physical activity (P < 0.05). The multivariate linear analysis showed that gender, history of drinking, and moderate and high physical activity are contributing factors to nausea and vomiting during chemotherapy (P < 0.05).

Conclusion

Moderate and high physical activity before chemotherapy is a protective factor for nausea and vomiting in patients with lung cancer. Physical activity can not only improve the degree of nausea and vomiting in patients with lung cancer but also reduce the incidence in these patients during the first chemotherapy cycle.
The effect of dysmenorrhea severity and interference on reactions to experimentally-induced pain
Authors: Rogers S. K. , Nichols K. L. , Ahamadeen N. , Shanahan M. L. , Rand K. L.
Published: July 2024

Introduction

Dysmenorrhea is associated with increased risk of chronic pain and hyperalgesia. Menstruating individuals with dysmenorrhea are more likely to have elevated pain reactivity when experiencing experimental pain, than those without. However, no study has examined intragroup differences in reactions to experimentally induced pain for individuals with dysmenorrhea. The main aim of this study was to examine the relative roles of dysmenorrhea severity and interference in the experience of experimentally-induced pain.

Methods

Participants were 120 menstruating individuals involved in a larger research study examining the influence of expectations on experimentally-induced pain. As part of the study, participants completed an online questionnaire regarding demographic and menstrual information and participated in a cold pressor task. Participants were randomized into four groups based on the manipulation of two independent variables: (1) high vs. low expectations about pain severity (pain-expectations); (2) and high vs. low expectations about one's pain tolerance (self-expectations). Participants verbally rated their pain severity throughout the cold pressor task using a 0–10 scale. Regression analyses were conducted examining the relationships between dysmenorrhea experience (i.e., average severity and interference) and cold pressor data [pain severity ratings and pain tolerance (i.e., total time in the cold pressor)], controlling for the manipulated expectations and age. Then, moderation analyses were conducted examining expectation group differences.

Results

When controlling for manipulated expectations and age, dysmenorrhea severity significantly predicted initial pain severity rating (p = 0.022) but did not predict final pain severity rating (p = 0.263) or pain tolerance (p = 0.120). Dysmenorrhea interference did not predict initial pain severity rating (p = 0.106), final pain severity rating (p = 0.134), or pain tolerance (p = 0.360). A moderation analysis indicated that the relationship between dysmenorrhea severity and initial pain severity rating was not moderated by pain-expectations, χ2(1) = 0.412, p = 0.521.

Discussion

During an experimentally-induced pain task, dysmenorrhea severity but not interference predicted initial pain severity rating, such that higher levels of dysmenorrhea severity predicted greater initial pain severity rating. This suggests individuals with more severe dysmenorrhea pain may experience greater initial sensitivity to pain and be at risk for increased sensitivity to acute pain and potentially the development of chronic pain.
Targeting dysregulated intracellular immunometabolism within synovial microenvironment in rheumatoid arthritis with natural products
Authors: Hu Shengtao , Lin Ye , Tang Yuanyuan , Zhang Junlan , He Yini , Li Gejing , Li Liqing , Cai Xiong
Published: July 2024

Immunometabolism has been an emerging hotspot in the fields of tumors, obesity, and atherosclerosis in recent decades, yet few studies have investigated its connection with rheumatoid arthritis (RA). In principle, intracellular metabolic pathways upstream regulated by nutrients and growth factors control the effector functions of immune cells. Dynamic communication and hypermetabolic lesions of immune cells within the inflammatory synovial microenvironment contributes to the development and progression of RA. Hence, targeting metabolic pathways within immune subpopulations and pathological cells may represent novel therapeutic strategies for RA. Natural products constitute a great potential treasury for the research and development of novel drugs targeting RA. Here, we aimed to delineate an atlas of glycolysis, lipid metabolism, amino acid biosynthesis, and nucleotide metabolism in the synovial microenvironment of RA that affect the pathological processes of synovial cells. Meanwhile, therapeutic potentials and pharmacological mechanisms of natural products that are demonstrated to inhibit related key enzymes in the metabolic pathways or reverse the metabolic microenvironment and communication signals were discussed and highlighted.
Research progress on nonsteroidal anti-inflammatory drugs in the treatment of pituitary neuroendocrine tumors
Authors: Li Jiaqi , Shi Xinkang , Tang Tao , Zhou Manxin , Ye Feng
Published: July 2024

Pituitary neuroendocrine tumor is the third most common primary intracranial tumor. Its main clinical manifestations include abnormal hormone secretion symptoms, symptoms caused by tumor compression of the surrounding pituitary tissue, pituitary stroke, and other anterior pituitary dysfunction. Its pathogenesis is yet to be fully understood. Surgical treatment is still the main treatment. Despite complete resection, 10%–20% of tumors may recur. While dopamine agonists are effective in over 90% of prolactinomas, prolonged use and individual variations can lead to increased drug resistance and a gradual decline in efficacy, which ultimately requires surgical intervention. Nonsteroidal anti-inflammatory drugs reduce the production of inflammatory mediator prostaglandins by inhibiting the activity of cyclooxygenase and exert antipyretic, analgesic, antiplatelet, and anti-inflammatory effects. In recent years, many in-depth studies have confirmed the potential of nonsteroidal anti-inflammatory drugs as a preventive and antitumor agent. It has been extensively utilized in the prevention and treatment of various types of cancer. However, their specific mechanisms of action still need to be fully elucidated. This article summarizes recent research progress on the expression of cyclooxygenase in pituitary neuroendocrine tumors and the treatment of nonsteroidal anti-inflammatory drugs. It provides a feasible theoretical basis for further research on pituitary neuroendocrine tumors and explores potential therapeutic targets.
Seriousness and outcomes of reported adverse drug reactions in old and new antiseizure medications: a pharmacovigilance study using EudraVigilance database
Authors: Girgis Michael Magdy Fahmy , Farkasinszky Gergely , Fekete Klára , Fekete István , Vecsernyés Miklós , Bácskay Ildikó , Horváth László
Published: July 2024

Introduction: Epilepsy is a widespread disease requiring long-term drug treatment. The aim of this study was to collect information on reported suspected adverse drug reactions (sADRs) of antiseizure medications (ASMs) and study their seriousness and outcomes in various system organ classifications (SOCs). We intended to compare old and new ASMs’ ADRs.

Methods: Using EudraVigilance (EV) database, we extracted line listings of reported sADRs with different ASMs over the period from January 2012 to December 2021. The list of ASMs was compiled according to the Anatomical therapeutic chemical classification system. The Medical Dictionary for Regulatory Activities version 24.0 was used for determining the SOCs of individual reported preferred terms (PTs) sADRs. In addition, we calculated the Reporting Odds Ratio (ROR), 95% confidence interval (95% CI), p-value (statistically significant if p< 0.05) and chi-square statistics.

Results: A total of 276,694 reports were contained in the exported line listings which included 1,051,142 individual sADRs reported as PTs such as seizure (3.49%), drug ineffective (2.46%), somnolence (1.32%), dizziness (1.29%) and represented four SOCs: nervous system disorders (19.26%), general disorders and administration site conditions (14.39%), psychiatric disorders (11.29%) and injury, poisoning and procedural complications (9.79). Among patients, the age group between 18 and 64 years had the highest percentage (52.40%), followed by those aged over 64 years (18.75%). Of all the reported PTs, 882,706 (83.98%) had reported seriousness. Old ASMs had a significant positive association with “caused/prolonged hospitalisation”, “congenital anomaly”, “disabling”, “life threatening” and “results in death”, while new ASMS with ‘other medically important condition’. There were 386 (0.04%) PTs related to Sudden Unexpected Death in Epilepsy (SUDEP).

Conclusion: In our study, we examined 10 years’ reported sADRs of ASMs in the EV international database. The majority of PTs were serious. Old ASMs were generally more commonly associated with undesired outcomes and seriousness. Considering their expected seriousness and outcomes, the safety profile of the different ASMs, can play a cardinal role in the selection of ASMs.
CBX2 enhances the progression and TMZ chemoresistance of glioma via EZH2-mediated epigenetic silencing of PTEN expression
Authors: Wang Jian , Yang Bo , Wang Yingzhao , Liu Shuhan , Ma Changkai , Piao Jianmin , Ma Shiqiang , Yu Dehai , Wu Wei
Published: July 2024

Chromobox (CBX) 2, a member of the CBX protein family and a crucial component of the polycomb repressive complex (PRC), exerts significant influence on the epigenetic regulation of tumorigenesis, including glioma. However, the precise role of CBX2 in glioma has remained elusive. In our study, we observed a substantial upregulation of CBX2 expression in glioma, which displayed a strong correlation with pathological grade, chemoresistance, and unfavorable prognosis. Through a series of in vivo and in vitro experiments, we established that heightened CBX2 expression facilitated glioma cell proliferation and bolstered resistance to chemotherapy. Conversely, CBX2 knockdown led to a significant inhibition of glioma cell growth and a reduction in chemoresistance. Notably, our investigation uncovered the underlying mechanism by which CBX2 operates, primarily by inhibiting PTEN transcription and activating the AKT/mTOR signalling pathway. Conversely, silencing CBX2 curtailed cell proliferation and attenuated chemoresistance by impeding the activation of the PTEN/AKT/mTOR signalling pathway. Delving deeper into the molecular intricacies, we discovered that CBX2 can recruit EZH2 and modulate the trimethylation of histone H3 lysine 27 (H3K27me3) levels on the PTEN promoter, effectively suppressing PTEN transcription. Our research unveils a comprehensive understanding of how CBX2 impacts the tumorigenesis, progression, chemoresistance, and prognosis of glioma. Furthermore, it presents CBX2 as a promising therapeutic target for drug development and clinical management of glioma.
Quasi-position vector curves in Galilean 4-space
Authors: Elsharkawy Ayman , Elsharkawy Noha
Published: July 2024

The Frenet frame is not suitable for describing the behavior of the curve in the Galilean space since it is not defined everywhere. In this study, an alternative frame, the so-called quasi-frame, is investigated in Galilean 4-space. Furthermore, the quasi-formulas in Galilean 4-space are deduced and quasi-curvatures are obtained in terms of the quasi-frame and its derivatives. Quasi-rectifying, quasi-normal, and quasi-osculating curves are studied in Galilean 4-space. We prove that there is no quasi-normal and accordingly normal curve in Galilean 4-space.
Conduction system pacing upgrade versus biventricular pacing on pacemaker-induced cardiomyopathy: a retrospective observational study
Authors: Pei-pei Ma , Ying Chen , Yi-heng Yang , Guo-cao Li , Cheng-ming Ma , Qing Fa , Lian-jun Gao , Yun-long Xia , Ying-xue Dong
Published: July 2024

Objective: The feasibility of the conduction system pacing (CSP) upgrade as an alternative modality to the traditional biventricular pacing (BiVP) upgrade in patients with pacemaker-induced cardiomyopathy (PICM) remains uncertain. This study sought to compare two modalities of CSP (His bundle pacing (HBP) and left bundle branch pacing (LBBP)) with BiVP and no upgrades in patients with pacing-induced cardiomyopathy.

Methods: This retrospective analysis comprised consecutive patients who underwent either BiVP or CSP upgrade for PICM at the cardiac department from 2017 to 2021. Patients with a follow-up period exceeding 12 months were considered for the final analysis.

Results: The final group of patients who underwent upgrades included 48 individuals: 11 with BiVP upgrades, 24 with HBP upgrades, and 13 with LBBP upgrades. Compared to the baseline data, there were significant improvements in cardiac performance at the last follow-up. After the upgrade, the QRS duration (127.81 ± 31.89 vs 177.08 ± 34.35 ms, p < 0.001), NYHA class (2.28 ± 0.70 vs 3.04 ± 0.54, p < 0.05), left ventricular end-diastolic diameter (LVEDD) (54.08 ± 4.80 vs 57.50 ± 4.85 mm, p < 0.05), and left ventricular ejection fraction (LVEF) (44.46% ± 6.39% vs 33.15% ± 5.25%, p < 0.001) were improved. There was a noticeable improvement in LVEF in the CSP group (32.15% ± 3.22% vs 44.95% ± 3.99% (p < 0.001)) and the BiVP group (33.90% ± 3.09% vs 40.83% ± 2.99% (p < 0.001)). The changes in QRS duration were more evident in CSP than in BiVP (56.65 ± 11.71 vs 34.67 ± 13.32, p < 0.001). Similarly, the changes in LVEF (12.8 ± 3.66 vs 6.93 ± 3.04, p < 0.001) and LVEDD (5.80 ± 1.71 vs 3.16 ± 1.35, p < 0.001) were greater in CSP than in BiVP. The changes in LVEDD (p = 0.549) and LVEF (p = 0.570) were similar in the LBBP and HBP groups. The threshold in LBBP was also lower than that in HBP (1.01 ± 0.43 vs 1.33 ± 0.32 V, p = 0.019).

Conclusion: The improvement of clinical outcomes in CSP was more significant than in BiVP. CSP may be an alternative therapy to CRT for patients with PICM. LBBP would be a better choice than HBP due to its lower thresholds.
Molecular identification of two thioredoxin genes and their function in antioxidant defense in Arma chinensis diapause
Authors: Shen Zhongjian , Luo Qiaozhi , Mao Jianjun , Li Yuyan , Wang Mengqing , Zhang Lisheng
Published: July 2024

Thioredoxin (Trx), an important part of thioredoxin systems, plays crucial role in maintaining the intracellular redox balance by scavenging reactive oxygen species (ROS). However, few Trxs have been functionally characterized in Arma chinensis, especially in diapause. In this study, diapause induction condition promoted hydrogen peroxide accumulation and increased CAT enzymatic activity and ascorbate content, suggesting that A. chinensis was exposed to high level of ROS. Therefore, we identified AcTrx2 and AcTrx-like, and investigated the relationship with antioxidant defense. It was found that AcTrx2 expression was significantly induced, whereas AcTrx-like expression was the highest on day 10 under diapause conditions. The expression of AcTrx2 and AcTrx-like in fat body, a central metabolic organ of resisting oxidative stress, was significantly increased under diapause conditions, and was significantly improved by 5/15°C (diapause temperature). We investigated the knockdown of AcTrx2 and AcTrx-like in A. chinensis and found that some selected antioxidant genes were upregulated, indicating that the upregulated genes may be functional compensation for AcTrx2 and AcTrx-like silencing. We also found that the enzymatic activities of SOD and CAT, and the metabolite contents of hydrogen peroxide, ascorbate increased after AcTrx2 and AcTrx-like knockdown. These results suggested the AcTrx2 and AcTrx-like may play critical roles in antioxidant defense of A. chinensis diapause.
Benefits and functionality of an interorganisational workplace health management network – insights from the companies’ perspective
Authors: Hente Luisa , Schlesinger Torsten
Published: July 2024

Introduction

Workplace health management (WHM) is a worthwhile investment for companies. Nevertheless, the implementation of health-promoting interventions remains limited, especially in small and medium-sized enterprises. Interorganisational networks could be a promising way to raise awareness of the advantages of implementing WHM. Therefore, the aim of this study is to analyse the perceived functionality and benefits of a regional WHM network from companies’ perspective and to present initial results on this specific topic.

Methods

An explorative qualitative case study was conducted analysing ERZgesund, a WHM network in a rural region in Germany. Twenty-two companies that participated in the network were interviewed about their experiences and perceived advantages and disadvantages participating in the WHM network ERZgesund.

Results

The findings show that the network has raised awareness about WHM among the companies, provides opportunities for exchange of knowledge and experiences, and generates or strengthens collaboration. The positive effects were enhanced by the network’s structure, such as regionality and a direct contact person. Nevertheless, some companies stated that they would welcome a higher level of participation and transparency.

Conclusion

Overall, it becomes clear that a WHM network can be a valuable tool to emphasize the relevance of WHM to companies. Therefore, further studies should validate and intensify the research on WHM networks to ensure a long-term benefit from the network.
Near miss road traffic accidents and associated factors among truck drivers in Gamo zone, southern Ethiopia by using a contributory factors interaction model
Authors: Bekelcho Tariku , Birgoda Gebremaryam Temesgen , Leul Hawi , Maile Maechel , Alemayehu Micheal , Olani Ararso Baru
Published: July 2024

Background

Road traffic accidents (RTAs) are among the leading causes of injuries, fatalities, and the resulting increase in financial burdens worldwide. Every year, RTAs cause numerous serious injuries and fatalities in Ethiopia. it is important to understand how prevalent near-miss crash accidents are, and which by definition could have injured the victim but did not result in an actual accident. The determinants of these near-misses are essential in road crash accident reduction strategies. In spite of the fact that near-miss accidents are much more common than actual losses or injuries, very little research has been conducted on them. Thus, this study was intended to assess the near-miss accidents and associated factors among truckers in Gamo zone, southern Ethiopia.

Methodology

The community-based cross-sectional study was employed from May 12 to July 10,2022, using a structured interviewer-administered questionnaire. A simple random sampling technique was used to select participants. The data were analyzed using the statistical package for social sciences. A binary and multivariate logistic regression model was used to identify the determinants of near-miss accidents. A statistical significance level was set at p < 0.05.

Results

About 72.5% of truckers had experienced near-miss road traffic accidents. The majority of the near-miss accidents were caused by speeding, followed by driving on the wrong side of the road and skidding, 65 (22.6%), 39 (13.5%), and 38 (13.2%), respectively. Driving frequency per week, location of accidents, condition of the road, sleeping status, and weather conditions were significantly associated with near-miss accidents.

Conclusion

The prevalence of near-miss accidents is high in the Gamo zone. Being a younger and less educated driver, high driving frequency per week, driving on major roads and junctions, foggy weather, and inadequate sleep all contribute to the occurrence of accidents. Road safety measures that could address these identified factors are required to mitigate potential RTAs.
Relationship between domain-specific physical activity and cognitive function in older adults – findings from NHANES 2011–2014
Authors: Wu Sijun , Wang Lin , Liu Shijie , Qi Juancai , Shi Fengrui , Zhuang Huiqi , Qian Youling , Mei Linqi , Zhang Maolin
Published: July 2024

Objective

To determine the relationship between domain-specific physical activity (PA) (e.g., occupational PA [OPA], transport-related PA [TPA], and recreational PA [RPA]) and cognitive function in older adults.

Methods

The data was obtained from the 2011–2014 cycle of the NHANES. We utilized weighted multivariate linear regression models among the included 2,924 people aged 60 years or older for our purposes.

Results

RPA and total PA according to WHO guidelines were associated with verbal fluency (RPA β: 1.400, 95% CI: 0.776, 2.024, p = 0.002; total PA β: 1.115, 95% CI: 0.571, 1.659, p = 0.001), processing speed and executive function (RPA β: 2.912, 95% CI. 1.291, 4.534, p = 0.005; total PA β: 2.974, 95% CI: 1.683, 4.265, p < 0.001) were positively correlated, and total PA was correlated with delayed memory performance (β: 0.254, 95% CI: 0.058, 0.449, p = 0.019). No significant association was observed between OPA, TPA, and various aspects of cognitive function among individuals over 60 years.

Conclusion

There was no noteworthy correlation discovered between OPA and TPA in relation to cognitive function. However, RPA and total PA exhibited significant associations with verbal fluency, processing speed, and executive function. Additionally, maintaining PA levels ranging from 600 to 1,200 MET-min/week would yield the most favorable outcomes for cognitive function.
How adverse childhood experiences impact the professional quality of life of residential care workers: resilience as a mediator for burnout, secondary traumatic stress, and compassion satisfaction
Authors: Milne Lise , Ratushniak Adrienne , Nguyen Hannah
Published: July 2024

Introduction

The well-being of trauma-affected children and youth in residential care settings is contingent upon the well-being of the workers who care for them, who are increasingly expected to provide care in a trauma-informed manner. The well-being of residential care workers (RCWs) may be impacted by their own histories of adversity, their capacity individually and collectively to navigate to resources that sustain their well-being (resilience), and current perceptions of their professional quality of life.

Objective

This study aimed to fill a research gap by canvassing the perspectives of RCWs to determine what and how they need to be supported in their work. We sought to better understand what personal (adverse childhood experiences, resilience) and professional (compassion satisfaction, burnout, secondary traumatic stress) experiences and capacities they bring into their work that might impact the quality of care they provide to children and youth.

Method

A sample of 226 residential care workers from four residential care organizations across three Canadian provinces completed a self-report questionnaire to provide a portrait of their history of adverse childhood experiences as measured by theACE questionnaire, which included two additional questions reflecting the more nuanced and expanded understanding of potential adversity in childhood in the Canadian context; their resilience, as measured by theAdult Resilience Measure; and compassion satisfaction, burnout, and secondary traumatic stress, as measured by theProfessional Quality of Life Measure. Mediation was conducted to examine whether and how resilience mediated the relationship between ACEs and professional quality of life indicators.

Results

(1) RCWs reported experiencing ACEs at rates much higher than general population and norm samples, especially regarding the experience of 4–5+ ACEs, known to be a threshold for increased severity in negative outcomes; (2) RCWs experienced levels of resilience and indicators of professional quality of life similar to those in other human services professions serving trauma-impacted individuals; and (3) RCW resilience significantly mediated the relationship between ACEs and compassion satisfaction, burnout, and secondary traumatic stress, and had a significant total effect for the relationship between ACEs and secondary traumatic stress. These results suggest the importance of enhancing RCW resilience in multiple ways, mainly in their professional contexts. Recommendations for resilience enhancement and suggestions for future research are provided.
Why Robots Should Grow Like Vines
Authors: McFarland Ciera , Fuentes Francesco , Fick Allison , Blumenschein Laura H. , Coad Margaret M.
Published: July 2024

When a vine grows up the side of a building or through a crack in the sidewalk, it lengthens from its tip. This allows it to easily grow around obstacles and squeeze through small holes. Its body forms a path back to its roots, where it draws water and nutrients from the soil to continue growing. This idea has inspired growing “vine robots”, soft, air-powered robots that lengthen from the tip by pushing out material stored inside their bodies. Acting like robotic plants, vine robots can move easily through cluttered areas like vines can, and they can also do robotic tasks, like carrying cameras and other tools. This combination can help doctors reach inside the human body, archeologists see inside ancient ruins, inspectors see inside pipes, and more. It is exciting to think about how robots that grow like vines will help people in the future.
Context-sensitivity and context-productivity: notions of “practice” and “practicality” in ethnomethodology and conversation analysis
Authors: Meyer Christian
Published: July 2024

The text reconstructs the concepts of practice and practicality used in ethnomethodology and conversation analysis and examines their internal similarities and differences as well as similarities and differences to other practice theories. After a description of the characteristics of practice theories, the ethnomethodological perspective on practice and practicality is presented. Then, the use of the terms in conversation analysis is examined. Ethnomethodology uses the notions of “practice” and “practicality” to outline a non-metaphysical theory of social order in which the sharedness of rules or meanings is not presupposed. “Practical” here means that social action, and social order more generally, are practically grounded as well as temporally and situationally constrained. The fact that practical action is fundamentally situated and can only be understood “from within” establishes an essentially indexical character of practical action. In conversation analysis, “practices” are viewed as “context-free” but “context-sensitive” components that constitute action and as such become the objects of investigation. While some have diagnosed a departure of conversation analysis from its ethnomethodological roots, I argue that “context-freeness” and “context-sensitivity” should be complemented by “context-productivity” by reference to Garfinkel’s interpretation of Aron Gurwitsch’s gestalt phenomenology in order to formulate a more encompassing concept of practice.
The use of cheese whey powder in the cultivation of protein-rich filamentous fungal biomass for sustainable food production
Authors: Kaya Burcu , Wijayarathna E. R. Kanishka B. , Yüceer Yonca Karagül , Agnihotri Swarnima , Taherzadeh Mohammad J. , Sar Taner
Published: July 2024

Cheese whey is an industrial by-product that is generated in excess during the cheese production process in the dairy industry. Despite the potential utility of whey, it continues to pose environmental threats in the industry. This study comprehensively evaluates the utilization of two fermentation techniques (solid-state fermentation and submerged fermentation) for producing fungal biomass from cheese whey powder, employing Aspergillus oryzae, Rhizopus oryzae, and Neurospora intermedia for sustainable food production. It has been observed that submerged fermentation is more effective in increasing the protein content of whey powder compared to solid-state fermentation. The highest biomass yield was achieved with A. oryzae (5.29 g/L, 0.176 g biomass/g substrate), followed by N. intermedia (3.63 g/L, 0.121 g biomass/g substrate), and R. oryzae (1.9 g/L, 0.063 g biomass/g substrate). In the bubble column reactor, the protein content of the substrate (78.65 g/kg) increased by 165.54 and 176.69% with A. oryzae (208.85 g/kg) and N. intermedia (217.62 g/kg), respectively. This study has demonstrated that whey powder can be converted into protein-rich biomass through fungal bioconversion. The obtained biomass has the potential to be developed as an alternative food and feed source, contributing to waste management and sustainable food production.
A comprehensive review of experimental models and induction protocols for avian necrotic enteritis over the past 2 decades
Authors: Shamshirgaran Mohammad Ali , Golchin Mehdi
Published: July 2024

Necrotic enteritis (NE) is a severe gastrointestinal disease that poses a significant threat to the poultry industry. It leads to progressive damage to the small intestine, reduced performance, increased mortality rates, and substantial economic losses. With the removal of antimicrobial agents from chicken feed, there is an urgent need to find alternative approaches for NE control. Various approaches, including vaccination, prebiotics, probiotics, and plant-derived products, have been utilized to address NE in poultry management. To evaluate the efficacy of these preventive measures against NE, successful induction of NE is crucial to observe effects of these approaches in related studies. This study presents a comprehensive overview of the methods and approaches utilized for NE reproduction in related studies from 2004 to 2023. These considerations are the careful selection of a virulent Clostridium perfringens strain, preparation of challenge inoculum, choice of time and the route for challenge inoculum administration, and utilization of one or more predisposing factors to increase the rate of NE occurrence in birds under experiment. We also reviewed the different systems used for lesion scoring of NE-challenged birds. By gaining clarity on these fundamental parameters, researchers can make informed decisions regarding the selection of the most appropriate NE experimental design in their respective studies.
Chitosan non-particulate vaccine delivery systems
Authors: Masimov Rasim , Wasan Ellen K.
Published: July 2024

Chitosan is an extensively used polymer for drug delivery applications in particulate and non-particulate carriers. Chitosan-based particulate, nano-, and microparticle, carriers have been the most extensively studied for the delivery of therapeutics and vaccines. However, chitosan has also been used in vaccine applications for its adjuvant properties in various hydrogels or as a carrier coating material. The focus of this review will be on the usage of chitosan as a vaccine adjuvant based on its intrinsic immunogenicity; the various forms of chitosan-based non-particulate delivery systems such as thermosensitive hydrogels, microneedles, and conjugates; and the advantages of its role as a coating material for vaccine carriers.
Labor developments and methods for supporting education in the Cyber-Physical and Intelligent Robot Systems Laboratory
Authors: Masuk Abdullah , Diós Szabolcs Sándor , Dinya Tamás , Szántó Attila , Husi Géza
Published: July 2024

This study investigates the education and laboratory market trends in the Cyber-Physical and Intelligent Robot Systems Laboratory and presents the previous works. The laboratory utilizes augmented reality, virtual reality, and simulation technologies to offer immersive learning experiences. The program emphasizes industry-aligned projects to prepare students and researchers for Industry 4.0. The curriculum teaches collaborative problem-solving and human-robot interaction. The lab trains flexible automation professionals for industry. The integration of technology and pedagogy empowers students to apply CPS and IRS concepts effectively. The laboratory is instrumental in advancing the educational landscape in these domains and is at the forefront of preparing students for the evolving challenges of automation technologies.
Investigation of the Real Efficiency of a Decentralized Heat Recovery Ventilation System
Authors: Tolvaly-Roșca Ferenc , Fekete Albert-Zsombor , Forgó Zoltán
Published: July 2024

The paper presents the measurement method and values of the temperatures of the incoming and outgoing air obtained as a result of the connection and automation of 4 decentralized heat recovery ventilation units into one system, and the measured results of the thermal efficiency of the entire system. The built system consists of four single-room heat recovery devices, which are integrated into an automated system. The built-in sensors can be used to measure the average temperature of the ventilated rooms or the current temperature and relative humidity of the air flowing through the ventilation units, as well as the carbon dioxide level of the rooms. The units received automation that complements the original individual control elements, which is capable of complex control tasks from any computing device, graphic interface and Wi-Fi connection. The units' sensors, which can be moved into the air stream, are suitable for the momentary measurement of the parameters of the flowing air, thereby estimating the thermal efficiency of the entire system.
The Antifungal and Inhibitory Effects of Massoia Essential Oil and C10 Massoia Lactone on Mycotoxin Production in Fusarium graminearum KACC 41047
Authors: Lee Jieun , Lee Sung-Eun
Published: July 2024

In wheat and barley, Fusarium head blight is mainly caused by Fusarium graminearum, and its control is based on the agricultural practices of not leaving crop residues in the field, growing phytopathogenic fungi-resistant varieties, biological control, and chemical treatment, including using fungicides. Here, we investigated the antifungal and antimycotoxigenic activities of Massoia essential oil (MEO) and C10 Massoia lactone (C10) on Fusarium graminearum KACC 41047. Because DMSO, which was used as a solvent in this study, exhibited antifungal activity at 5% in a fungal growth medium, it was used in the antifungal and antimycotoxigenic experiments at 0.05%. Three assays were used to investigate the antifungal activities of MEO and C10, which exhibited potent antifungal activity in the agar dilution assay, with complete fungal growth inhibition at 100 mg/L. At 5–50 mg/L, MEO and C10 suppressed deoxynivalenol and 15-acetyl-deoxynivalenol production by >50% by downregulating the Tri10 gene, which expresses trichodiene synthase. MEO and C10 might be potent antifungal agents for F. graminearum control with less toxicological concerns because they are GRAS chemicals.
Plant Disease: A Growing Threat to Global Food Security
Authors: Gai Yunpeng , Wang Hongkai
Published: July 2024

The escalating global population has led to an increased demand for both quantity and quality in food production. Throughout history, plant diseases have posed significant threats to agricultural output by causing substantial food losses annually while also compromising product quality. Accurate identification of pathogens, clarifying the pathogenic mechanism of pathogens, and understanding the interaction between pathogens and hosts are important for the control of plant diseases. This Special Issue, “Research Progress on Pathogenicity of Fungi in Crops”, belongs to the section “Pest and Disease Management” of Agronomy. It contains research papers on the identification and phylogeny of fungal pathogens, the molecular genetics of plant fungal pathogens, the molecular mechanisms of fungal pathogenicity, and the molecular basis of the interaction between fungi and crops. These studies encapsulate efforts to understand disease systems within current genomics, transcriptomics, proteomics, and metabolomics studies, highlighting research findings that could be future targets for crop disease and pest control. The studies presented in this Special Issue promote the progress of fungal pathogenicity research in crops and provide a scientific basis for future disease control, which is of great significance for sustainable agricultural development and global food security.
A Precise Plot-Level Rice Yield Prediction Method Based on Panicle Detection
Authors: Wei Junshuo , Tian Xin , Ren Weiqi , Gao Rui , Ji Zeguang , Kong Qingming , Su Zhongbin
Published: July 2024

Accurately estimating rice yield is essential for ensuring global food security, enhancing agricultural productivity, and promoting agricultural economic growth. This study constructed a dataset of rice panicles at different growth stages and combined it with an attention mechanism and the YOLOv8 network to propose the YOLOv8s+LSKA+HorNet rice panicle detection and counting model, based on a drone remote sensing platform. Using the panicle count data collected by this model, along with the thousand-grain weight, number of grains per panicle, and actual yield data from a rice nitrogen gradient experimental field, various machine learning models were trained to ultimately propose a field-level rapid rice yield estimation model, RFYOLO. The experimental results show that the rice panicle detection and counting model can achieve an average precision (AP) of 98.0% and a detection speed of 20.3 milliseconds. The final yield estimation model achieved a prediction R2 value of 0.84. The detection and counting model significantly reduced missed and duplicate detections of rice panicles. Additionally, this study not only enhanced the model’s generalization ability and practicality through algorithmic innovation but also verified the impact of yield data range on the stability of the estimation model through the rice nitrogen gradient experiment. This is significant for early rice yield estimation and helping agricultural producers make more informed planting decisions.
Regional Winter Wheat Yield Prediction and Variable Importance Analysis Based on Multisource Environmental Data
Authors: Xu Hao , Yin Hongfei , Liu Yaohui , Wang Biao , Song Hualu , Zheng Zhaowen , Zhang Xiaohu , Jiang Li , Wang Shuai
Published: July 2024

Timely and accurate predictions of winter wheat yields are key to ensuring food security. In this research, winter wheat yield prediction models for six provinces were established using a random forest (RF) model. Two methods were employed to analyze feature variables. RF partial dependence plots were generated to demonstrate the nonlinear relationships between the feature variables and yield, and bivariate Moran’s I was considered to identify the spatial associations between variables. Results showed that when environmental data from key growth periods were used for prediction model establishment, the root mean square error (RMSE) varied between 200 and 700 kg/ha, and the coefficient of determination (R2) exceeded 0.5. Feature variable analysis results indicated that the longitude, latitude, topography and normalized difference vegetation index (NDVI) were important variables. Below the threshold, the yield gradually increased with increasing NDVI. Bivariate Moran’s I results showed that there was zonal distribution of meteorological elements. Within a large spatial range, the change in environmental variables due to the latitude and longitude should be accounted for in modeling, but the influence of collinearity between the feature variables should be eliminated via variable importance analysis.
Biofilm Formation and Antibiotic Resistance Profiles in Carbapenemase-Producing Gram-Negative Rods—A Comparative Analysis between Screening and Pathological Isolates
Authors: Vintilă Camelia , Coșeriu Răzvan Lucian , Mare Anca Delia , Ciurea Cristina Nicoleta , Togănel Radu Ovidiu , Simion Anastasia , Cighir Anca , Man Adrian
Published: July 2024

(1) Background: Carbapenem-resistant (CR) bacteria pose a significant global public health challenge due to their ability to evade treatment with beta-lactam antibiotics, including carbapenems. This study investigates the biofilm-forming capabilities of CR clinical bacterial isolates and examines the impact of serum on biofilm formation. Additionally, the study evaluates the resistance profiles and genetic markers for carbapenemase production. (2) Methods: Bacterial isolates were collected from the microbiology laboratory of Mures County Clinical Hospital between October 2022 and September 2023. Pharyngeal and rectal swabs were screened for carbapenem-resistant bacteria using selective media. Lower respiratory tract samples were also analyzed for CR Gram-negative bacteria. The isolates were tested for their ability to form biofilms in the presence and absence of fetal bovine serum at 24 and 48 h. Carbapenemase production was detected phenotypically and confirmed via PCR for relevant genes. (3) Results: Out of 846 screened samples, 4.25% from pharyngeal swabs and 6.38% from rectal swabs tested positive for CR bacteria. Acinetobacter baumannii and Klebsiella pneumoniae were the most common species isolated. Biofilm formation varied significantly between clinical isolates and standard strains, with clinical isolates generally showing higher biofilm production. The presence of serum had no significant effect on biofilm formation in Klebsiella spp., but stimulated biofilm formation for Acinetobacter spp. Carbapenemase genes blaKPC, blaOXA-48-like, and blaNDM were detected in various isolates, predominantly in Klebsiella spp., but were not the main determinants of carbapenem resistance, at least in screening isolates. (4) Conclusions: This study highlights the variability in biofilm formation among CR clinical isolates and underscores the differences between the bacteria found as carriage versus infection. Both bacterial species and environmental factors variably influence biofilm formation. These insights are crucial for the development of effective treatment and infection control strategies in clinical settings.