bluuebunny/crossref_metadata_2025 huggingface dataset:
RELASI KEPALA DESA DAN BPD DALAM PROSES PEMBANGUNAN LOS PASAR
Authors: Berek Emanuel Yansen , Festianto Dian , Kolne Yakobus
Published: March 2022

Masalah dalam penelitian ini adalah relasi BPD dan Kepala desa dalam tahap MusrembangDes, Proses tujuan dalam pembentukan PerDes, dan Proses pembentukan panitia pembangunan. Oleh karena itu penelitian ini untuk mendeskripsikan relasi kepala desa dan BPD dalam proses pembangunan. Metode penelitian yakni metode deskriptif dengan pendekatan kualitatif. Teknik pengumpulan data: observasi, wawancara dan dokumentasi. Teknik anallisa data menggunakan teknik analisis interaktif. Hasil penelitian menunjukkan bahwa, relasi Kepala Desa dan Badan Permusyawaratan Desa (BPD) dalam tahap musrembangDes, Pembentukan PerDes dan proses pembentukan Panitia Pembangunan belum berjalan dengan baik dikarenakan bebepa faktor yang mempengaruhinya. Oleh karena itu direkomendasikan beberapa alternatif diantaranya: 1) Dalam proses perencanaan pembangunan Los pasar ditinjau dari partisipasi masyarakat dalam pelaksanaan Musrenbangdes di Desa Bani-bani pemerintah harus mengoptimalkan dengan mengupayakan berbagai macam cara untuk merangsang masyarakat untuk berpartisipasi melalui musrenbangdes seperti persiapan, pelaksanaan, Pasca Musrenbangdes, 2) Pemerintah desa bani-bani harus membangun hubungan yang  baik  dengan  Badan  Permusyawaratan Desa (BPD) sehingga proses pembangunan yang direncanakan bisa berjalan dengan  baik,  3) Harus  adanya koordinasi yang baik antara pemerintah desa dengan lembaga BPD sehingga tidak terjadi pemahaman di antara kedua lembaga tersebut, dan 4) Harus adanya sosialisasi dari pemerintah   desa, terkait perencanaan pembangunan yang akan direalisasikan sehinnga masyarakat bisa mengetahui tujuan dari pembangunan.
OLIGARKI MEDIA DALAM PEMILIHAN UMUM PRESIDEN 2019
Authors: Dahlia Rembulan Randu
Published: March 2022

Penelitian ini membahas tentang aspek politik oligarki media dalam pemilihan presiden 2019 (Pilpres). Fokus penelitian adalah faktor dukungan oligarki media pada kandidat Joko Widodo dan Ma’ruf Amin. Penelitian ini perlu dilakukan untuk memaparkan faktor pendorong oligark dalam mendukung kandidat, serta cara oligark merealisasikannya. Penelitian ini diharapkan menjadi rujukan pembuatan kebijakan dalam kepemiluan, khususnya pada tahap kampanye, Oligarki media dalam penelitian ini adalah Harry Tanoesoedibjo melalui MNC Media dan Partai Persatuan Indonesia (Perindo). Dukungan Harry Tanoe berubah pada Pilpres 2014 Harry Tanoe mendukung Prabowo Subianto – Hatta Rajasa bukan Jokowi-Jusuf Kalla, sementara saat Pilpres 2019 Hary Tanoe mendukung Jokowi-Maruf. Berdasarkan Laporan Penerimaan dan Pengeluaran Dana Kampanye (LPPDK) Pilpres 2019, Harry Tanoe menjadi oligark media penyumbang dana kampanye terbesar melalui sumbangan perseorangan, perusahaan dan Perindo dengan total sumbangan mencapai Rp 31,39 Milyar (Sumber: KPU RI). Analisis penelitian ini menggunakan Teori Oligarki Jeffrey Winters (2011) yang menyatakan oligark perlu masuk dalam struktur politik untuk mempertahakan kekayaan dan variasi motif oligark dalam mendukung kandidat. Penelitian ini menggunakan metode kualitatif dengan argumen yang ditawarkan: Motif dukungan oligarki media dalam Pilpres 2019 menjelaskan cara oligark mempertahankan kekayaan dan merealisasikannya.
ECOS DEL CARACOL
Authors: Aguilar Lomelí Rafael Francisco , Aranda Mata Juan Manuel , Arteaga Novoa Jaime , Avelar González Luis , Bernal Acevedo Rolando , Camacho Sandoval Salvador , Collazo Alejandro , Contreras Alonso Adán , Cruz Palomino Mario , Delgado López Juan Carlos , Estrada Dávila Derly , García Zapata Javie , González Rivas José de Jesús , Guerra Armas Zeus Hamlet , Guerra Espinoza Rogelio , Gutiérrez Romo José de Jesús , Lomelí Llamas Elías , Méndez Parga María Gabriela , Meza Medina Gustavo , Moreno Ramos Víctor , Oliva Mares Abel , Quiroz Benítez Armando , Ramírez Oliva José , Rodríguez Orozco Demetrio , Valdivia Magdaleno Fortino , Alonso de Alba Armando , Alcalá López Efraín , Aranda Delgado María Magdalena , Aranda Montoya Fátima , Ávila Villareal Manuel de Jesús , Camino Gutiérrez Karla Andrea , Consuelo Tamayo Jesús , de la Torre Alcocer José , F. Jaramillo Nathalia , Guerrero Castorena José Trinidad , Llanes López Julio Miguel , Meza Martínez Carmen Lucía , Brigitte Nájera Jeannette , Olvera Romero Caleb , Quezada Rodríguez Claudia Patricia , ECOS DEL CARACOL Rodríguez Reyes Claudia Soraya
Published: March 2022

En 1994 nació La Cofradía, conformada por un grupo de personas que optamos por la compañía franca y generosa, creativa y alegre. El nombre fue puesto de manera lúdica, aludiendo a las hermandades cristianas o a los gremios medievales. En un principio, el propósito de La Cofradía era reunirnos para participar en el mundo de las artes, ahora, después de un cuarto de siglo, los integrantes del grupo también nos juntamos para celebrar la vida, que es maravillosa. Esta antología reúne voces con una diversidad de temas, estilos y géneros que permiten un acercamiento flexible y sugestivo; integra 42 autores, 42 voces, 42 universos que se mueven con imaginación lúdica y creadora. Quienes aquí escriben se saben herederos históricos de miles y miles de voces, y se proyectan con arrojo y sueños hacia un futuro incierto y retador, deseosos, quizás.
THE ROLE OF THE VILLAGE GOVERNMENT IN THE MANAGEMENT OF VILLAGE BUSINESS AGENCIES (BUMDES)
Authors: Pananrangi M A.
Published: March 2022

[Full Paper] to pdf

Copyright (c) 2020 Meraja journal
Implementasi Algoritma AES untuk Keamanan Data Rekam Medis
Authors: Khoirunnisa Oktaria Gina , Djuniadi Djuniadi
Published: March 2022

Pertumbuhan teknologi yang sangat pesat tentunya sangat mempengaruhi berbagai bidang. Berbagai pekerjaan sehari hari pun juga terbantu dengan adanya teknologi. Selain dampak positif yang diperhatikan dampak negatif pun juga perlu diwaspadai  yaitu perihal keamanan dokumen. Bidang kesehatan merupakan contoh yang bidang berhubungan dengan privasi. Salah satunya yaitu dokumen rekam medis. Tidak sedikit kasus kebocoran data pasien yang terjadi. Peristiwa yang kurang diinginkan seperti ini dapat terhindarkan dengan melakukan pengamanan dengan Kriptografi. Terdapat berbagai macam kriptografi salah satunya yaitu AES (Advanced Encryption Standard). Berdasarkan uraian tersebut penelitian ini bertujuan untuk melakukan simulasi pengamanan  dokumen rekam medis pada salah satu klinik dengan menggunakan metode AES. Hasil simulasi menunjukan bahwa algoritma AES dapat menjadi rekomendasi bagi perlindungan data. Dengan melalui proses enkripsi data akan diubah menjadi bentuk yang tidak dimengerti dan harus diproses menggunakan proses dekripsi agar bisa kembali dimengerti. Keuntungannnya adalah tidak semua orang bisa mengakses dokumen tersebut.
Klasifikasi Sinyal EEG Menggunakan Model K-Nearest Neighbor Untuk Pengenalan Kata Yang Dibayangkan
Authors: Rauf Abdul , Yosrita Efy , Aziza Rosida Nur
Published: March 2022

Locked in syndrome (LIS) is a condition of complete paralysis in which people with LIS are conscious but unable to move or communicate verbally except to move their eyes or blink. One way that can help LIS sufferers to communicate and interact is through recording brain signals called Electroencephalogram (EEG). In this study, the data from the recording of the EEG signal has gone through the extraction stage. The extracted data is preprocessed and classified using the K-Nearest Neighbor (K-NN) algorithm to be visualized using a web-based application. The results of the classification using the K-Nearest Neighbor algorithm with a value of K = 1 resulted in 82% accuracy, 82% precision and 82% recall.

Keywords: LIS, EEG, K-Nearest Neighbor.
Perancangan Purwarupa Pendeteksian Masker Menggunakan Mobilenetv2 dan Sensor Suhu GY-906 MLX-90614 Berbasis OpenCV
Authors: Silalahi Lukman Medriavin , Antoyo David Martin , Budiyanto Setiyo , Simanjuntak Imelda Uli Vistalina , Osman Gunawan , Rochendi Agus Dendi , Sutiadi Raden
Published: March 2022

The background of this research problem is the handling of the Covid-19 virus by using masks and regular body temperature measurements for each user entering/exiting a building. So this research aims to monitor people in carrying out the principle of using a mask and detecting a person's temperature with a limit not exceeding the normal temperature of 37.5 selsius. Outside of this research is the design of a face mask detection system and can measure a person's body temperature using Python programming that contains OpenCV, MobileNetV2 and gy-906 MLX 90614 non contact temperature sensors. the results of this research can be concluded that face mask detection can be done in the range of 50cm to 1.5m while face detection can be detected up to 3m when conducting real time testing and has a success rate of detecting face masks 86.6% to 93.3% from 15 times the experiments that have been conducted.
Rancang Bangun Sistem Monitoring dan Controlling Penggunaan Daya Peralatan Listrik Rumah Tangga Menggunakan IoT
Authors: Muhammad Alwi , Prayitno Budi , Putra Rakhmadi Irfansyah , Putra Eka , Palupiningsih Pritasari
Published: March 2022

Sektor rumah tangga menyumbang persentase terbesar dari total konsumsi listrik Indonesia pada tahun 2019. Pengguna di sektor ini tidak mengetahui secara detail peralatan rumah tangga mana yang paling banyak mengkonsumsi listrik. Penelitian ini bertujuan untuk menghasilkan sistem kontrol dan monitoring peralatan listrik rumah tangga dengan paradigma internet of things. Proses pemantauan dan pengendalian dilakukan untuk mendukung beberapa perangkat rumah tangga. Pada penelitian ini metode yang digunakan untuk membuat prototype alat dengan beberapa tahapan yaitu pembuatan prototype alat, pengumpulan data, dan pembuatan aplikasi mobile. Bagian utama dari perangkat prototipe terdiri dari mikrokontroler NodeMCU, sensor PZEM004T, dan relay. Hasil pembacaan sensor berupa data arus, tegangan, dan daya listrik yang kemudian dikirim dan disimpan dalam database. Setelah itu, data listrik dapat ditampilkan pada aplikasi mobile. Selain itu, juga dapat mengontrol on/off perangkat. Hasil dari penelitian ini adalah prototype perangkat dan aplikasi mobile yang dapat mengontrol dan memonitoring listrik untuk peralatan kelistrikan di rumah. Konsumsi listrik setiap alat listrik rumah tangga dapat dipantau dalam kilowatt-hour (KWH), sehingga pengguna dapat menggunakannya untuk menilai konsumsi listrik rutin peralatan listrik rumah tangga mereka.
Perancangan Aplikasi Pembelian Air Minum Isi Ulang Menggunakan QR Code Berbasis Android
Authors: Syamsul Syamsul , Aziza Rosida Nur , Yosrita Efy , Ningrum Rahma Farah
Published: March 2022

Tujuan penelitian ini untuk mengembangkan sebuah aplikasi transaksi pembelian air minum isi ulang otomatis berbasis Android. Hal ini di motivasi berdasarkan kebutuhan masyarakat pengguna botol air minum isi ulang (tumbler), yang kesulitan untuk mengisi kembali isi tumblernya ketika berada di area publk seperti stasiun kereta api, bandara, terminal bis, dll. Hal ini mengakibatkan pengguna tumbler tetap mengkonsumsi minuman dengan kemasan sekali pakai, sehingga program pengurangan sampah plastic kurang efektif. Hasil penelitian berupa aplikasi yang menrapakan  kode QR untuk  pemanggilan aplikasi  Android. Pengguna mendatangi  terminal, semacam vending machine, dan melakukan transaksi pembelian air minum isi ulang melalui aplikasi di telepon selulernya, dimulai dari pemilihan volume sampai dengan metode pembayaran
PELATIHAN GOOGLE APPS SEBAGAI PENUNJANG ADMINISTRATIF DI DESA BANDARSARI
Authors: Jupriyadi Jupriyadi , Nurkholis Andi , Budiman Arief , Pasha Donaya , Ahdan Syaiful , Gusbriana Ellin , Sobirin Muhammad Hamdan
Published: March 2022

Administrasi desa memegang peranan yang penting karena keterlibatan pemerintah dalam sistem administrasi. Untuk itu agar tujuan pemerintah benar-benar dapat tercapai seperti yang diharapkan, maka yang harus diperhatikan yaitu adanya aparat pemerintah yang memiliki kualitas yang memadai. Untuk menyelenggarakan administrasi desa yang efektif diperlukan pembinaan dan pengawasan yang dilakukan oleh pemerintah kecamatan terhadap aparatur pemerintah desa dalam bidang pemerintahan, sehingga perangkat desa dapat melakukan tugas dan kewajibannya dengan baik dalam melayani masyarakat. Keberadaan pemerintah desa merupakan alat untuk mencapai tujuan pembangunan nasional ataupun sebagai lembaga yang memperkuat struktur pemerintahan negara Indonesia. Penyelenggaraan Pemerintahan di Desa Bandarsari sudah berjalan selama dua tahun, tapi dalam pelaksanaanya masih ada beberapa hal yang perlu diperhatikan oleh Pemerintah Desa.
PENERAPAN WEBSITE DESA KUNJIR KECAMATAN RAJA BASA
Authors: Damayanti Damayanti , Rusliyawati Rusliyawati , Susanto Erliyan Redi , Budiman Arief , Novita Dian , Bahtiar Ayu Febriani , Mahendra Anton
Published: March 2022

Akibat adanya pandemi COID-19 ini membuat masyarakat terhambat dalam menjalankan aktivitas salah satunya memperoleh informasi ke perangkat desa. Selain itu pandemi COVID-19 telah melumpuhkan aktivitas-aktivitas pertumbuhan perekonomian pembanguna desa. Desa wisata Kunjir merupakan salah satu desa yang terletak di Lampung Selatan. Desa Kunjir memiliki potensi pertumbuhan perekonomian dibidang pariwisata. Potensi di desa Kunjir ini belum banyak diketahui oleh berbagai wisatawan. Analisis permasalahan yang ditemukan oleh tim pengabdi yaitu belum adanya website untuk menyebarkan informasi tentang desa Kunjir. Tim pengabdi melakukan PKM ini dengan membuat website di desa Kunjir agar website dapat mengenalkan desa Kunjir dengan berbagai informasi mengenai potensi di desa Kunjir yang bertujuan untuk membangkitkan perekonomian yang ada di desa Kunjir.  Website ini merupakan upaya mengenalkan atau penyebaran informasi tentang desa Kunjir dengan memanfaatkan penyebaran informasi melalui teknologi yang dapat digunakan oleh perangkat desa.
SISTEM INFORMASI PEMERINGKATAN PRESTASI SISWA BERBASIS WEB PADA SMK PELITA PESAWARAN
Authors: Satria Muhammad Najib Dwi
Published: March 2022

Pelita Pesawaran Vocational School is a university that has a myriad of achievements by students, both academic and non-academic. In reporting every year there is an increase in terms of the number of achievements that have been achieved, so that some adjustments to conditions are needed. Student achievements that have been achieved should actually be archived into a system that can be processed in order to obtain accurate and relevant results. Therefore we need a system that can process achievement data accurately and integrated and can display the ranking of student achievement at the level of study programs, faculties and university levels. With the creation of a system entitled Web-Based Student Achievement Rating System at Pelita Pesawaran Vocational School, it is hoped that it will be able to help Pelita Pesawaran Vocational School in processing achievement data every year and be able to rank student achievement so that Pelita Pesawaran Vocational School can obtain accurate and relevant information on outstanding students in accordance with the data provided. there is. The Web-Based Student Achievement Ranking System at Pelita Pesawaran Vocational School is designed using PHP, CSS, JavaScript, and HTML programming languages which are used as interfaces while for data storage media using MySql.languages which are used as interfaces while for data storage media using MySql.
PELATIHAN PENGARSIPAN SECARA ELEKTRONIK (E-FILLING) BAGI PERANGKAT DESA DI PEKON SUKANEGERI JAYA
Authors: Darwis Dedi , Sulistiani Heni , Isnain Auliya Rahman , Yasin Ikbal , Hamidy Fikri , Mersita Rika , Mega Elvano Delisa
Published: March 2022

This service activity in the form of electronic archiving training or e-filling is motivated by archiving activities carried out at the village office still using the traditional way, namely using paper. This method will cause many problems, so it is necessary to conduct electronic archiving training for village officials. The village office in Pekon Sukanegeri Jaya has been equipped with computer equipment and an internet network that can be used for electronic archive management. However, the skills and knowledge of village officials are still lacking, especially for archiving using Google Drive media. The purpose of implementing this service is that with the existence of electronic archiving training for village officials, it is hoped that archive management at the village office can be more organized and make it easier for village officials to re-search the archive files needed at any time.
MANAGING INDONESIA TO BECOME THE WORLD MARITIME AXIS
Authors: Kristiyanti, S.Kom, M.M, Mariana , Fauziningrum Endah
Published: March 2022

Wilayah Indonesia yang paling luas adalah lautan. Jadi, bukan tidak mungkin menjadikan Indonesia sebagai pusat maritim dunia atau bahkan sebagai poros maritim dunia, seperti yang kita impikan. Dalam sejarah Amanna Gappa disebutkan bahwa hukum maritim dunia berasal dari Nusantara. Hal tersebut menjadi latar belakang yang kuat bagi Indonesia untuk bangkit melalui maritim. Untuk itu diperlukan pengelolaan laut. Tanpa pengelolaan laut yang terstruktur dan terintegrasi, impian untuk membangun Indonesia melalui maritim hanya akan menjadi mimpi. Ini menjadi manfaat karena anugerah Tuhan terbesar Indonesia adalah lautan yang merupakan dua pertiga dari letak geografis Indonesia. Untuk memanfaatkannya, Indonesia harus memiliki pengelolaan laut yang khas. Ini mencakup konsep pertahanan, keamanan dan keselamatan. Ini adalah konsep kedaulatan tentang pembatasan dan pengaturan di laut. Karena ada perbedaan konsep darat dan laut. Ada pagar atau batas yang jelas di darat. Sebaliknya, laut adalah perairan terbuka lebar tanpa batas. Landasan Indonesia sebagai poros maritim dunia diharapkan dengan mengoptimalkan pengelolaan laut.

 

Kata kunci : Manajemen, Maritim, Poros maritim

 
Elementary Students’ Attitudes towards an Online Visiting Lecture in Covid-19 School Closure Period
Authors: Aryanti Aryanti , Wijaya Tommy Tanu
Published: March 2022

This study aims to describe the attitudes of elementary students towards online visiting lectures and teacher problems while teaching in the Covid-19 school closure period. The visiting lecture was conducted to relieve the boredom of online learning. The novelty of online visiting lecture conducted at elementary school level in Covid-19 school closure period. This study sample comprised  48 third grade students at a private elementary school in Bandung, West Java, Indonesia. This study used a qualitative approach. The data were collected through observation and interviews with elementary school students. The results showed that the students' attitudes towards online visiting lecture in Covid-19 school closure period were very good. The students engaged in learning actively. When the visiting lecturer asked questions, the students answered the questions confidently. In conclusion, the visiting lecture can be held in Covid-19 school closure period when students need a new online learning atmosphere. In Indonesia, Covid-19 pandemic has lasted more than 1 year, and it causes boredom of online learning. Holding visiting lecture is an effective way to increase students’ learning interest and motivation so that the online teaching and learning process runs more efficiently.
The Colour of Fashion at the Salon du Goût Français: A Virtual Exhibition of French Luxury Commodities, 1921–1923
Authors: Blackman Cally
Published: March 2022

This article investigates the use of the Autochrome, an important photographic process invented by the Lumière brothers that produced the most accurate representation of colour between 1907 and the early 1930s, in a government-backed exhibition of French luxury commodities, the Salon du Goût Français. Between 1921 and 1923 the exhibition showed in Paris and undertook two international tours, first to North America and then to Australasia, China, Vietnam, Japan and India. Thousands of objects were displayed, from automobiles to umbrellas, including couture, ready to wear, lingerie, menswear, children's wear and accessories. By reducing the objects to two dimensions on the glass Autochrome plates, the exhibition could be shown in a relatively small venue in Paris, transported to America in a trunk and voyage on a decommissioned battle cruiser to the Far East. Using the trope of Western fashion as a form of soft power mediated by the global reach afforded by the Autochromes, the article proposes that the Salon du Goût Français offered a kind of roving virtual art gallery, a vividly colourful encyclopaedic display of over 2,000 images of luxury manufacturing deployed to restore France's imperial and cultural hegemony as supreme arbiter of taste after the trauma of the First World War.
ÜREME ÇAĞINDAKİ KADINLARIN COVİD 19 AŞISI İLE İLGİLİ OKUR YAZARLIK DÜZEYLERİ
Authors: DOĞAN YÜKSEKOL Özlem , BALTACI Nazlı , NAZİK Feyza , DUMAN Mesude
Published: March 2022

Araştırma, üreme çağındaki kadınların Covid-19 aşısına yönelik okuryazarlık düzeylerini belirlemek amacıyla tanımlayıcı ve kesitsel olarak yapıldı. Araştırmanın örneklemini üreme çağında olan toplam 609 kadın oluşturdu. Veriler, araştırmacılar tarafından literatür taranarak oluşturulan “Kişisel Bilgi Formu” ve “Covid-19 Aşı Okuryazarlık Ölçeği (AOÖ)” ile toplandı. Kadınların Covid-19 AOÖ’den aldıkları toplam puan ortalaması 2.83±0.47, ölçeğin Fonksiyonel Beceriler Alt Boyutundan aldıkları puan ortalaması 2.61±0.70 ve İletişimsel/Eleştirel Beceriler Alt Boyutundan aldıkları puan ortalaması 2.94±0.61 olarak belirlendi. Kadınların aşı okuryazarlık düzeyleri orta idi. Kadınların aşı okuryazarlık düzeylerini artıracak eğitimlerin yapılması ve projeler geliştirilmesi önerilebilir.
Valorisation of firewood
ash waste for fired clay ceramics production
Authors: Santos Filipe , Bagli Siqueira Fabrício , Nilson França Holanda José
Published: March 2022

The objective of this work was to investigate the
valorisation of firewood ash waste as an alternative raw material for clay
ceramic production, through replacement of industrial plastic clay up to 20
wt.%. For this purpose, clay ceramic pieces were produced by uniaxial pressing
and fired at temperatures ranging from 800 °C to 1100 °C. The technical
properties of the fired clay ceramic pieces have been determined. It was found
that the technical properties of the clay ceramic pieces depend on the firewood
ash waste amount and firing temperature. The fired pieces incorporated with up
to 20 wt.% of firewood ash waste showed a water absorption level of 9.90 to
16.62 % and flexural strength of 4.12 to 12.02 MPa, indicating their conformity
for clay ceramic production (bricks and roofing tiles). Obtained results
confirmed the feasibility of valorisation of firewood ash waste in the
production of fired clay ceramics.
Implementation of MALDI-TOF Mass Spectrometry to Identify Fungi From the Indoor Environment as an Added Value to the Classical Morphology-Based Identification Tool
Authors: Motteu Natacha , Goemaere Berdieke , Bladt Sandrine , Packeu Ann
Published: March 2022

Introduction

During the last decades, molds in the indoor environment have raised concern regarding their potential adverse health effects. The genera Aspergillus, Cladosporium, Penicillium, Alternaria, and yeasts, the most common fungi found indoors, include species with high allergenic and toxigenic potentials. Identification of these molds is generally performed by microscopy. This method has, however, some limitations as it requires mycologists with high expertise while identification is often limited to the genus level. Therefore, it is necessary to seek for fast and accurate tools, such as Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDITOF MS), enabling an identification to the species level and guiding general practitioners in their search for the underlying cause of a health problem.

Methods

In this study, 149 fungal air and dust isolates from 43 dwellings in Brussels were taken in collaboration with Brussels Environment RCIB/CRIPI and identified by both microscopy and MALDI-TOF MS in Sciensano's Indoor Mycology laboratory. Spectra obtained via MALDI-TOF MS were compared with data available in an in-house created reference database containing over 1,700 strains from the BCCM/IHEM fungal collection.

Results

A total of 149 isolates including 18 yeasts and 131 filamentous fungi were analyzed. Microscopic analysis indicated 18 yeast species and allowed identification of 79 isolates (53%) to the genus level. Only 36 isolates (24%) could be identified to the species complex level. Fifteen molds (10%) could not be identified, and one was indicated as sterile mycelia. No isolate was identified to species level. MALDI-TOF MS analysis identified 137 (92%) of the 149 isolates with a logscore > 1.7. Of these 137 isolates, 129 (87%) were identified to the species level (logscore > 2.0). For only 8 isolates (5%), identification was limited to the genus/section level (1.7 < logscore <2.0), and 12 isolates (8%) could not be identified.

Conclusion

A comparison of results obtained with both methods indicates an increased precision in identifications with MALDI-TOF MS analysis for 92% of the isolates, emphasizing its highly added value to the standard microscopic analysis in routine practice. In addition, MALDI-TOF MS also enables to assess the accuracy of microscopic identifications.
A microRNA Cluster-Lefty Pathway is Required for Cellulose Synthesis During Ascidian Larval Metamorphosis
Authors: Sun Xueping , Zhang Xiaoming , Yang Likun , Dong Bo
Published: March 2022

Synthesis of cellulose and formation of tunic structure are unique traits in the tunicate animal group. However, the regulatory mechanism of tunic formation remains obscure. Here, we identified a novel microRNA cluster of three microRNAs, including miR4018a, miR4000f, and miR4018b in Ciona savignyi. In situ hybridization and promoter assays showed that miR4018a/4000f/4018b cluster was expressed in the mesenchymal cells in the larval trunk, and the expression levels were downregulated during the later tailbud stage and larval metamorphosis. Importantly, overexpression of miR4018a/4000f/4018b cluster in mesenchymal cells abolished the cellulose synthesis in Ciona larvae and caused the loss of tunic cells in metamorphic larvae, indicating the regulatory roles of miR4018a/4000f/4018b cluster in cellulose synthesis and mesenchymal cell differentiation into tunic cells. To elucidate the molecular mechanism, we further identified the target genes of miR4018a/4000f/4018b cluster using the combination approaches of TargetScan prediction and RNA-seq data. Left–right determination factor (Lefty) was confirmed as one of the target genes after narrow-down screening and an experimental luciferase assay. Furthermore, we showed that Lefty was expressed in the mesenchymal and tunic cells, indicating its potentially regulatory roles in mesenchymal cell differentiation and tunic formation. Notably, the defects in tunic formation and loss of tunic cells caused by overexpression of miR4018a/4000f/4018b cluster could be restored when Lefty was overexpressed in Ciona larvae, suggesting that miR4018a/4000f/4018b regulated the differentiation of mesenchymal cells into tunic cells through the Lefty signaling pathway during ascidian metamorphosis. Our findings, thus, reveal a novel microRNA-Lefty molecular pathway that regulates mesenchymal cells differentiating into tunic cells required for the tunic formation in tunicate species.
Hyperspectral Video Analysis by Motion and Intensity Preprocessing and Subspace Autoencoding
Authors: Vitale Raffaele , Ruckebusch Cyril , Burud Ingunn , Martens Harald
Published: March 2022

Hyperspectral imaging has recently gained increasing attention from academic and industrial world due to its capability of providing both spatial and physico-chemical information about the investigated objects. While this analytical approach is experiencing a substantial success and diffusion in very disparate scenarios, far less exploited is the possibility of collecting sequences of hyperspectral images over time for monitoring dynamic scenes. This trend is mainly justified by the fact that these so-called hyperspectral videos usually result in BIG DATA sets, requiring TBs of computer memory to be both stored and processed. Clearly, standard chemometric techniques do need to be somehow adapted or expanded to be capable of dealing with such massive amounts of information. In addition, hyperspectral video data are often affected by many different sources of variations in sample chemistry (for example, light absorption effects) and sample physics (light scattering effects) as well as by systematic errors (associated, e.g., to fluctuations in the behaviour of the light source and/or of the camera). Therefore, identifying, disentangling and interpreting all these distinct sources of information represents undoubtedly a challenging task. In view of all these aspects, the present work describes a multivariate hybrid modelling framework for the analysis of hyperspectral videos, which involves spatial, spectral and temporal parametrisations of both known and unknown chemical and physical phenomena underlying complex real-world systems. Such a framework encompasses three different computational steps: 1) motions ongoing within the inspected scene are estimated by optical flow analysis and compensated through IDLE modelling; 2) chemical variations are quantified and separated from physical variations by means of Extended Multiplicative Signal Correction (EMSC); 3) the resulting light scattering and light absorption data are subjected to the On-The-Fly Processing and summarised spectrally, spatially and over time. The developed methodology was here tested on a near-infrared hyperspectral video of a piece of wood undergoing drying. It led to a significant reduction of the size of the original measurements recorded and, at the same time, provided valuable information about systematic variations generated by the phenomena behind the monitored process.
Mussel-Inspired Magnetic Dissolving Pulp Fibers Toward the Adsorption and Degradation of Organic Dyes
Authors: Yang Jiawei , Lu Shengchang , Wu Hui , Hu Huichao , Miao Qingxian , Huang Liulian , Chen Lihui , Ni Yonghao
Published: March 2022

In this work, a simple synthetic method was used to prepare a new type of magnetic dissolving pulp (MDP) @polydopamine (PDA) fibers. The hydroxyl groups of the fibers were converted into carboxyl groups after succinylation. Fe3O4 nanoparticles were grown in situ on the fibers. The prepared MDP@PDA fibers have catalytic reduction efficiency and adsorption performance for methylene blue organic dyes, and it has been thoroughly tested under various pH conditions. Fe3O4@PDA fibers have high reusability, are easy to separate, and regenerate quickly. The catalytic and adsorption efficiency barely decreases after repeated use. The surface of dissolving pulp fibers with a functionalized multifunctional PDA coating is used to create multifunctional catalysts and adsorbent materials. This study presents a very useful and convenient method for the synthesis and adjustment of MDP@PDA fibers, which have a wide range of potential applications in catalysis and wastewater treatment.
Synthetic Red Blood Cell-Specific Glycolytic Intermediate 2,3-Diphosphoglycerate (2,3-DPG) Inhibits Plasmodium falciparum Development In Vitro
Authors: Morais Inês , Medeiros Márcia M. , Carvalho Maria , Morello Judit , Teixeira Sara M. , Maciel Suelma , Nhantumbo Janice , Balau Ana , Rosa Margarida T. G. , Nogueira Fátima , Rodrigues João Alexandre , Carvalho Filomena A. , Antunes Alexandra M. M. , Arez Ana Paula
Published: March 2022

Mechanisms of malaria parasite interaction with its host red blood cell may provide potential targets for new antimalarial approaches. Pyruvate kinase deficiency has been associated with resistance to malaria in both experimental models and population studies. Two of the major pyruvate kinase deficient-cell disorders are the decrease in ATP and the increase in 2,3-biphosphoglycerate (2,3-BPG) concentration. High levels of this metabolite, only present in mammalian red blood cell, has an inhibitory effect on glycolysis and we hypothesized that its accumulation may also be harmful to the parasite and be involved in the mechanism of protection provided by that enzymopathy. We examined the effect of a synthetic form, 2,3-DPG, on the Plasmodium falciparum intraerythrocytic developmental cycle in vitro. Results showed an impairment of parasite growth with a direct effect on parasite maturation as significant lower progeny emerged from parasites that were submitted to 2,3-DPG. Further, adding the compound to the culture medium did not result in any effect on the host cell, but instead the metabolic profile of an infected cell became closer to that of a non-infected cell.
Modeling Feedback in Interaction With Conversational Agents—A Review
Authors: Axelsson Agnes , Buschmeier Hendrik , Skantze Gabriel
Published: March 2022

Intelligent agents interacting with humans through conversation (such as a robot, embodied conversational agent, or chatbot) need to receive feedback from the human to make sure that its communicative acts have the intended consequences. At the same time, the human interacting with the agent will also seek feedback, in order to ensure that her communicative acts have the intended consequences. In this review article, we give an overview of past and current research on how intelligent agents should be able to both give meaningful feedback toward humans, as well as understanding feedback given by the users. The review covers feedback across different modalities (e.g., speech, head gestures, gaze, and facial expression), different forms of feedback (e.g., backchannels, clarification requests), and models for allowing the agent to assess the user's level of understanding and adapt its behavior accordingly. Finally, we analyse some shortcomings of current approaches to modeling feedback, and identify important directions for future research.
Aerial Survey Robotics in Extreme Environments: Mapping Volcanic CO2 Emissions With Flocking UAVs
Authors: Ericksen John , Fricke G. Matthew , Nowicki Scott , Fischer Tobias P. , Hayes Julie C. , Rosenberger Karissa , Wolf Samantha R. , Fierro Rafael , Moses Melanie E.
Published: March 2022

We present methods for autonomous collaborative surveying of volcanic CO2 emissions using aerial robots. CO2 is a useful predictor of volcanic eruptions and an influential greenhouse gas. However, current CO2 mapping methods are hazardous and inefficient, as a result, only a small fraction of CO2 emitting volcanoes have been surveyed. We develop algorithms and a platform to measure volcanic CO2 emissions. The Dragonfly Unpiloted Aerial Vehicle (UAV) platform is capable of long-duration CO2 collection flights in harsh environments. We implement two survey algorithms on teams of Dragonfly robots and demonstrate that they effectively map gas emissions and locate the highest gas concentrations. Our experiments culminate in a successful field test of collaborative rasterization and gradient descent algorithms in a challenging real-world environment at the edge of the Valles Caldera supervolcano. Both algorithms treat multiple flocking UAVs as a distributed flexible instrument. Simultaneous sensing in multiple UAVs gives scientists greater confidence in estimates of gas concentrations and the locations of sources of those emissions. These methods are also applicable to a range of other airborne concentration mapping tasks, such as pipeline leak detection and contaminant localization.
The Influence of the Vanishing Twin on the Perinatal Outcome of Surviving Singleton in IVF Pregnancy
Authors: Li Jiarong , Li Jingyu , Zhang Yiyuan , Hu Kuona , Chen Na , Gao Jie , Hu Jingmei , Cui Linlin , Chen Zi-Jiang
Published: March 2022

Objective

The purpose of this study was to clarify the influence of the vanishing twin (VT) on the perinatal outcomes in the surviving singleton and further identify the susceptible window.

Study design

Retrospective cohort study.

Methods

A total of 636 survivors of a vanished co-twin and 11,148 singleton controls were enrolled. The exposed group was further divided into early VT (EVT, VT ≤13 weeks, N = 593) and late VT subgroups (LVT, VT >13 weeks, N = 43) according to the gestational age of the twin vanishing. All participants were conceived through in vitro fertilization (IVF). Perinatal outcomes including gestational age, birthweight, and the incidence of preterm birth (PTB), low birthweight (LBW), small for gestational age (SGA), neonatal intensive care unit (NICU) admission, umbilical cord abnormality, jaundice of the newborn, and oligohydramnios were compared among the groups.

Results

In our birth cohort, about 5.4% of all singleton deliveries originated from vanishing twin pregnancies. Compared with the singletons, both early and late VT pregnancy had a significantly lower birth weight (3337.57±532.24 g and 2916.05±526.07 g vs. 3446.15±526.07 g; p < 0.001 and p < 0.001), more frequent neonatal jaundice (47.0% and 60.5% vs. 40.6%; p = 0.002 and p = 0.008), and decreased incidence of umbilical cord abnormality (15.5% and 7.0% vs. 19.9%; p = 0.009 and p = 0.034). Newborns in the early VT group were more likely to manifest as SGA (5.4% vs. 3.6%, p = 0.002) and suffered oligohydramnios (5.4% vs. 3.4%; p = 0.008) than the primary singletons. In addition, the gestational age of late VT survivors was shorter than that of the controls (37.25 ± 3.25 vs. 39.04 ± 1.63, p = 0.001) and had a significantly higher risk of PTB (30.2% vs. 6.6%; p < 0.001) and NICU admission (27.9% vs. 9.4%, p < 0.001). All differences except for SGA maintain significance after adjusting for maternal age, BMI, and parity.

Conclusions

Singletons with a vanished co-twin had worse perinatal outcomes compared with the original singletons, with LVT burden even much on the survival one. Therefore, close monitoring during the perinatal period was suggested in this type of neonates. Moreover, elective single embryo transfer should also be fully considered which could tackle the problem at its root.
Energy-Efficiency-Oriented Vision Feedback Control of QCSP Systems: Linear ADRC Approach
Authors: Li Shengming , Feng Lin
Published: March 2022

How to save the energy of unmanned aerial vehicles (UAVs) and then enable long-distance transport is a very real and difficult task. However, for UAVs, the classic object detection algorithm, such as the deep convolutional neural network–based object detection algorithm and the classic flight control algorithm, such as the PID-based position control algorithm, require significant energy, which limits the application scenarios of the UAV system. In view of this problem, this paper proposes a lightweight object detection network and a linear active disturbance rejection controller (LADRC) for the quadrotor with the cable-suspended payload (QCSP) system to improve energy efficiency. The system uses a YOLOV3 network and embeds it into the Jesson NX mobile platform to accurately detect the target position. Furthermore, a nonlinear velocity controller with a cable-suspended structure to control the velocity of the payload, a LADRC algorithm is adopted to achieve fast and accurate control of the payload position. Simulations and real flight experiments show that the proposed object detection algorithm and the LADRC control strategy can save the energy of drone effectively.
A Quantitative Assessment on Ecological Compensation Based on Water Resources Value Accounting: A Case Study of Water Source Area of the Middle Route of South-To-North Water Transfer Project in China
Authors: Chen Junfei , Wang Qian , Li Qian
Published: March 2022

As an economic means to adjust the contradiction between ecology and development, ecological compensation plays an important role in promoting the good operation of interbasin water transfer projects and the sustainable development of regional economy. The accounting of ecological compensation is the key and difficult point of ecological compensation as well as the basis of ecological compensation policy and practice. Watershed ecological compensation based on water resources value accounting is an early exploration field of ecological compensation research, and water resources value calculation needs to consider both water quantity and water quality comprehensively. Taking the water source area of the Middle Route of South-to-North Water Transfer Project (SNWTP) as an example, this article tries to establish the payment standard of watershed ecological compensation from the perspective of water resources value. The results show that: 1) The water resources value of the six core regions in the water source area has shown an overall upward trend since 2000, and the northern regions have demonstrated higher value of water resources than the southern regions. 2) The LSTM neural network model is used to forecast the value of water resources in the six regions from 2020 to 2022, and it is found that the value of water resources would increase in the next few years. 3) The compensation price of the six regions in the water source area is predicted in the range of 0.5–1.5 yuan/m3 from 2020 to 2022, and an upward trend in the ecological compensation amount is forecast. Based on the above conclusions, this article puts forward suggestions to establish an ecological compensation accounting system in line with the Middle Route water source area from the perspectives of ecological compensation legislation, allocation of ecological compensation amount, and introduction of market mechanism.
A New Homotetramer Hemoglobin in the Pulmonary Surfactant of Plateau Zokors (Myospalax Baileyi)
Authors: Li Jimei , An Zhifang , Wei Linna , Xu Bo , Wang Zhijie , Gao Conghui , Wei Lian , Qi Delin , Shi Peng , Zhang Tongzuo , Wei Dengbang
Published: March 2022

The plateau zokor (Myospalax baileyi) is a native species to the Qinghai-Tibetan Plateau, inhabiting hypoxia and hypercapnia sealed subterranean burrows that pose several unique physiological challenges. In this study, we observed a novel heme-containing protein in the pulmonary surfactant (PS) of plateau zokor, identified the encoding gene of the protein, predicted its origination and structure, verified its expression in alveolar epithelial cells, and determined the protein’s affinity to oxygen and its effect on the oxygen-dissolving capability in the PS of plateau zokors. The protein is an unusual homotetramer hemoglobin consisting of four γ-like subunits, and the subunit is encoded by a paralog gene of γ, that is γ-like. The divergence time of γ-like from γ is estimated by the molecular clock to be about 2.45 Mya. The generation of γ-like in plateau zokors might well relate to long-time stress of the high land hypoxia. Unlike γ, the γ-like has a hypoxia response element (HRE) and a lung tissue-specific enhancer in its upstream region, and it is expressed specifically in lung tissues and up-regulated by hypoxia. The protein is named as γ4-like which is expressed specifically in Alveolar epithelial type II (ATII) cells and secreted into the alveolar cavities through the osmiophilic multilamellar body (LBs). The γ4-like has a higher affinity to oxygen, and that increases significantly oxygen-dissolving capability in the PS of plateau zokors by its oxygenation function, which might be beneficial for the plateau zokors to obtain oxygen from the severe hypoxia environments by facilitating oxygen diffusion from alveoli to blood.
Increasing Taxa Sampling Provides New Insights on the Phylogenetic Relationship Between Eriobotrya and Rhaphiolepis
Authors: Dong Zhanghong , Qu Shaohong , Landrein Sven , Yu Wen-Bin , Xin Jing , Zhao Wenzhi , Song Yu , Tan Yunhong , Xin Peiyao
Published: March 2022

Eriobotrya (Rosaceae) is an economically important genus with around 30 species. It is widely distributed in tropical and warm temperate regions of Asia, with most of its species in China, Myanmar, and Vietnam. However, Eriobotrya is often confused with the smaller genus Rhaphiolepis, and the phylogenetic relationships between the two genera are controversial. Here we present phylogenetic analyses of 38 newly generated Eriobotrya and Rhaphiolepis nrDNA together with 16 sequences of nrDNA and 28 sequences of ITS obtained from GenBank, representing 28 species of Eriobotrya and 12 species of Rhaphiolepis, in order to reconstruct highly supported relationships for the two genera. Contrary to previous research based on limited sampling, our results highlight the monophyly of Eriobotrya as well as Rhaphiolepis. The topology recovered here is consistent with key morphological synapomorphies such as the persistent sepals in Eriobotrya. Our findings show that increased sampling of taxa can provide a more robust phylogeny through reducing phylogenetic error and increasing overall phylogenetic accuracy.
SSH2.0: A Better Tool for Predicting the Hydrophobic Interaction Risk of Monoclonal Antibody
Authors: Zhou Yuwei , Xie Shiyang , Yang Yue , Jiang Lixu , Liu Siqi , Li Wei , Abagna Hamza Bukari , Ning Lin , Huang Jian
Published: March 2022

Therapeutic antibodies play a crucial role in the treatment of various diseases. However, the success rate of antibody drug development is low partially because of unfavourable biophysical properties of antibody drug candidates such as the high aggregation tendency, which is mainly driven by hydrophobic interactions of antibody molecules. Therefore, early screening of the risk of hydrophobic interaction of antibody drug candidates is crucial. Experimental screening is laborious, time-consuming, and costly, warranting the development of efficient and high-throughput computational tools for prediction of hydrophobic interactions of therapeutic antibodies. In the present study, 131 antibodies with hydrophobic interaction experiment data were used to train a new support vector machine-based ensemble model, termed SSH2.0, to predict the hydrophobic interactions of antibodies. Feature selection was performed against CKSAAGP by using the graph-based algorithm MRMD2.0. Based on the antibody sequence, SSH2.0 achieved the sensitivity and accuracy of 100.00 and 83.97%, respectively. This approach eliminates the need of three-dimensional structure of antibodies and enables rapid screening of therapeutic antibody candidates in the early developmental stage, thereby saving time and cost. In addition, a web server was constructed that is freely available at http://i.uestc.edu.cn/SSH2/.