bluuebunny/crossref_metadata_2025 huggingface dataset:
Combination Therapy of Alpha-Lipoic Acid, Gliclazide and Ramipril Protects Against Development of Diabetic Cardiomyopathy via Inhibition of TGF-β/Smad Pathway
Authors: Dugbartey George J. , Wonje Quinsker L. , Alornyo Karl K. , Robertson Louis , Adams Ismaila , Boima Vincent , Mensah Samuel D.
Published: March 2022

Background: Diabetic cardiomyopathy (DCM) is a major long-term complication of diabetes mellitus, accounting for over 20% of annual mortality rate of diabetic patients globally. Although several existing anti-diabetic drugs have improved glycemic status in diabetic patients, prevalence of DCM is still high. This study investigates cardiac effect of alpha-lipoic acid (ALA) supplementation of anti-diabetic therapy in experimental DCM.

Methods: Following 12 h of overnight fasting, 44 male Sprague Dawley rats were randomly assigned to two groups of healthy control (n = 7) and diabetic (n = 37) groups, and fasting blood glucose was measured. Type 2 diabetes mellitus (T2DM) was induced in diabetic group by intraperitoneal (i.p.) administration of nicotinamide (110 mg/kg) and streptozotocin (55 mg/kg). After confirmation of T2DM on day 3, diabetic rats received monotherapies with ALA (60 mg/kg; n = 7), gliclazide (15 mg/kg; n = 7), ramipril (10 mg/kg; n = 7) or combination of the three drugs (n = 7) for 6 weeks while untreated diabetic rats received distilled water and were used as diabetic control (n = 9). Rats were then sacrificed, and blood, pancreas and heart tissues were harvested for analyses using standard methods.

Results: T2DM induction caused pancreatic islet destruction, hyperglycemia, weight loss, high relative heart weight, and development of DCM, which was characterized by myocardial degeneration and vacuolation, cardiac fibrosis, elevated cardiac damage markers (plasma and cardiac creatine kinase-myocardial band, brain natriuretic peptide and cardiac troponin I). Triple combination therapy of ALA, gliclazide and ramipril preserved islet structure, maintained body weight and blood glucose level, and prevented DCM development compared to diabetic control (p < 0.001). In addition, the combination therapy markedly reduced plasma levels of inflammatory markers (IL-1β, IL-6 and TNF-α), plasma and cardiac tissue malondialdehyde, triglycerides and total cholesterol while significantly increasing cardiac glutathione and superoxide dismutase activity and high-density lipoprotein-cholesterol compared to diabetic control (p < 0.001). Mechanistically, induction of T2DM upregulated cardiac expression of TGF-β1, phosphorylated Smad2 and Smad3 proteins, which were downregulated following triple combination therapy (p < 0.001).

Conclusion: Triple combination therapy of ALA, gliclazide and ramipril prevented DCM development by inhibiting TGF-β1/Smad pathway. Our findings can be extrapolated to the human heart, which would provide effective additional pharmacological therapy against DCM in T2DM patients.
Simultaneous Dual Echo Gadolinium Enhanced MR-PET for Evaluation of PET Tracer Delivery in Altered Pathophysiology
Authors: Cowin Gary J , Mardon Karine , Houston Zachary H. , Bhalla Rajiv , Stimson Damion H. R. , Thurecht Kristofer J. , Brereton Ian M.
Published: March 2022

Efficacy of diagnostics and therapeutics for brain tumours can be modulated by vascular delivery and blood brain barrier permeability. Simultaneous dynamic gadolinium MR-PET enables independent assessment of vascular delivery and blood brain barrier integrity in a brain tumour animal model in the presence of a PET tracer.

Dual echo dynamic gadolinium enhanced gradient echo imaging allows simultaneous calculation of T2* and T1 images from the TE image pairs. Relaxivity values then enabled determination of independent T2*- and T1-derived gadolinium concentrations simultaneously with measurement of [18F]DPA-714 neuroinflammation radiotracer delivery.

Separate T2*- and T1-derived gadolinium concentrations curves were derived in a selection of tumours and normal tissue, reflecting vascular delivery and tissue uptake. Changes in the PET activity curves were seen in tumours and normal tissue, reflecting changes in the MR derived dynamic curves. The dramatic changes in the MR-derived vascular delivery and tissue uptake estimates may improve the understanding of the alteration of delivery and uptake of new theranostic agents.
The Impact of Physical Distancing in the Pandemic Situation: Considering the Role of Loneliness and Social Brain
Authors: Morese Rosalba , Longobardi Claudio
Published: March 2022

The new normal has made social distancing a new way of experiencing sociality. Social neuroscience has for a long time been concerned with studying the beneficial effects of social relationships, of physical contact. It is known that physical contact activates neurophysiological processes that reduce the perception of discomfort and even physical pain. So in the absence of physical contact, our social brain may be modulated differently when we are with others. But what could be the long-term effects of this normality? This mini-review focuses on highlighting with the support of social neuroscience evidence such as isolation, distancing can affect people’s health.
Do Improvements in Therapeutic Game-Based Skills Transfer to Real Life Improvements in Children's Emotion-Regulation Abilities and Mental Health? A Pilot Study That Offers Preliminary Validity of the REThink In-game Performance Scoring
Authors: David Oana A. , Magurean Silvia , Tomoiagă Cristina
Published: March 2022

Therapeutic or serious games are considered innovative ways of delivering psychological interventions especially suited for children and adolescents, which can have a positive impact on mental health, while also being fun and easily accessible online. While most serious games for children and adolescents address specific issues, such as anxiety or depression, preventive measures received less attention. REThink is an online therapeutic game designed as a stand-alone prevention tool, aiming to increase resilience in healthy children and adolescents in a Rational Emotive Behavioral Therapy framework (David et al., 2019). The aim of this pilot study was to investigate the validity of in-game performance measurements or scores as indicators of the game effectiveness in building real life emotion-regulation abilities. We analyzed how scores of different game levels (addressing different skills) are associated with improvements in mental health and emotion regulation abilities. Our preliminary results suggest that in-game performance at some levels (scores) consistently reflect improvements in psychological functioning, while in-game performance at other levels are less associated with changes in real life self-reported psychological functioning. These results offer important information about which levels can be used as preliminary indicators of psychological improvements, and which levels need to be revised in terms of task or scoring. Overall, results of our study offer preliminary validation of REThink's game scoring system, while also suggesting the elements to be refined.
The Attitudes and Perceptions of Israeli Psychiatrists Toward Telepsychiatry and Their Behavioral Intention to Use Telepsychiatry
Authors: Kaphzan Hanoch , Sarfati Noiman Margaret , Negev Maya
Published: March 2022

Background

Although telemedicine care has grown in recent years, telepsychiatry is growing at a slower pace than expected, because service providers often hamper the assimilation and expansion of telepsychiatry due their attitudes and perceptions. The unified theory of acceptance and use of technology (UTAUT) is a model that was developed to assess the factors influencing the assimilation of a new technology. We used the UTAUT model to examine the associations between the attitudes and perceptions of psychiatrists in Israel toward telepsychiatry and their intention to use it.

Methods

An online, close-ended questionnaire based on a modified UTAUT model was distributed among psychiatrists in Israel. Seventy-six questionnaires were completed and statistically analyzed.

Results

The behavioral intention of Israeli psychiatrists to use telepsychiatry was relatively low, despite their perceptions of themselves as capable of high performance with low effort. Nonetheless, they were interested in using telepsychiatry voluntarily. Experience in telepsychiatry, and to a lesser extent, facilitating conditions, were found to be positively correlated with the intention to use telepsychiatry. Psychiatrists have a positive attitude toward treating patients by telepsychiatry and perceive its risk as moderate.

Discussion

Despite high performance expectancy, low effort expectancy, low perceived risk, largely positive attitudes, high voluntariness, and the expectancy for facilitating conditions, the intention to use telepsychiatry was rather low. This result is explained by the low level of experience, which plays a pivotal role. We recommend promoting the facilitating conditions that affect the continued use of telepsychiatry when initiating its implementation, and conclude that it is critical to create a sense of success during the initial stages of experience.
Relationship Between Somatic Cell Counts and Mammary Gland Parenchyma Ultrasonography in Buffaloes
Authors: Zhang Xinxin , Ahmad Muhammad Jamil , An Zhigao , Niu Kaifeng , Wang Wei , Nie Pei , Gao Shan , Yang Liguo
Published: March 2022

The aim of the present study was to determine whether the echotextural features of the mammary gland parenchyma in buffaloes during lactation at different somatic cell levels could be used to diagnose mastitis. This study was divided into two parts. In the first experiment, experimental buffaloes (n = 65) with somatic cell counts (SCC) tests (n = 94) in different seasons, including spring (n = 22), summer (n = 24), autumn (n = 37), and winter (n = 11), were used to obtain ultrasonic variables for each quarter of mammary gland that could best explain the corresponding somatic cell level. In the second part of the study, the first part's experimental results were verified by subjecting at least one-quarter udder of eight buffaloes to ultrasonography seven times during mid-July to mid-August for obtaining ultrasonic values at different somatic cell levels. The echo textural characteristics [mean numerical pixel values (NPVs) and pixel heterogeneity (pixel standard deviation, PSD)] were evaluated using 16 ultrasonographic images of each buffalo with Image ProPlus software. The effects of SCC, days in milk (DIM), scanning order (SO), season, as well as the scanning plane and udder quarter (SP + UQ) on both the PSD and NPVs of the mammary gland were significant (p < 0.05). The correlation coefficient between pre-milking sagittal PSD and somatic cell score (SCS) was the highest (r = 0.4224, p < 0.0001) with fitted linear model: y = 0.19445x (dependent variable: SCS, independent variables: pre-milking sagittal PSD; R2 = 0.84, p < 0.0001). In addition, SCC and ultrasonic of udder quarter were followed for 1 month, confirming that pre-milking sagittal PSD of mammary gland value could explain the SCC variation in milk. The current study demonstrated that the ultrasonographic examination of the udder could be one of the complementary tools for diagnosing subclinical mastitis in buffaloes.
COVID-19 Vaccination Among the Arab Bedouin Population: Lessons Learned From a Minority Population
Authors: Abu-Freha Naim , Alsana Hadel , El-Saied Sabri , Azbarga Zuya , Aloka Muhammad , Goda Tarek , Abu Tailakh Muhammad
Published: March 2022

Objectives: We investigated the causes of low COVID-19 vaccination rates among a minority population and highlighted interventions for increasing the vaccination rate.

Methods: We reviewed the experience during the mass vaccination campaign period among the Arab Bedouin (AB) in Israel, attempting to determine important causes of low vaccination rates and gathered information from real-life experience and through direct contact with the population during the campaign.

Results: Causes for low vaccination rates in the AB are related to the health system infrastructure, crisis management strategies, and population characteristics. Long-standing socioeconomic inequalities, limited resources, and language and culture barriers present special challenges to the task of COVID-19 vaccination campaigns. Key interventions for increasing vaccination rates among minority populations include raising awareness, improving vaccination access, and directly targeting risk-groups. To maximize the effectiveness of these interventions they should be culturally adapted and executed according to the needs of each individual target community.

Conclusion: Culturally adapted awareness campaigns, interventions, and improved access to vaccines can be accomplished by cooperation between relevant governing and community bodies to increase COVID-19 vaccination rates among minorities.
Dynamic Line Scan Thermography Parameter Design via Gaussian Process Emulation
Authors: Verspeek Simon , De Boi Ivan , Maldague Xavier , Penne Rudi , Steenackers Gunther
Published: March 2022

We address the challenge of determining a valid set of parameters for a dynamic line scan thermography setup. Traditionally, this optimization process is labor- and time-intensive work, even for an expert skilled in the art. Nowadays, simulations in software can reduce some of that burden. However, when faced with many parameters to optimize, all of which cover a large range of values, this is still a time-consuming endeavor. A large number of simulations are needed to adequately capture the underlying physical reality. We propose to emulate the simulator by means of a Gaussian process. This statistical model serves as a surrogate for the simulations. To some extent, this can be thought of as a “model of the model”. Once trained on a relative low amount of data points, this surrogate model can be queried to answer various engineering design questions. Moreover, the underlying model, a Gaussian process, is stochastic in nature. This allows for uncertainty quantification in the outcomes of the queried model, which plays an important role in decision making or risk assessment. We provide several real-world examples that demonstrate the usefulness of this method.
A Review on the Performance of Linear and Mixed Integer Two-Stage Stochastic Programming Software
Authors: Torres Juan J. , Li Can , Apap Robert M. , Grossmann Ignacio E.
Published: March 2022

This paper presents a tutorial on the state-of-the-art software for the solution of two-stage (mixed-integer) linear stochastic programs and provides a list of software designed for this purpose. The methodologies are classified according to the decomposition alternatives and the types of the variables in the problem. We review the fundamentals of Benders decomposition, dual decomposition and progressive hedging, as well as possible improvements and variants. We also present extensive numerical results to underline the properties and performance of each algorithm using software implementations, including DECIS, FORTSP, PySP, and DSP. Finally, we discuss the strengths and weaknesses of each methodology and propose future research directions.
Insecticidal Effect of Pistacia lentiscus (Anacardiaceae) Metabolites against Lobesia botrana (Lepidoptera: Tortricidae)
Authors: Dasenaki Ioanna , Betsi Petri-Christina , Raptopoulos Dimitris , Konstantopoulou Maria
Published: March 2022

The extensive use of synthetic insecticides in agriculture poses a great risk for human health and the ecosystem, and mandates the development of safer alternatives derived from natural products. In the present study, we assessed the larvicidal effect of Pistacia lentiscus fruits, leaves, and bark extracts and their components on larvae of a major vine pest, Lobesia botrana. Pistacia lentiscus is an evergreen shrub or small tree possessing significant medicinal value with numerous therapeutic uses since antiquity. Using petri dish residual exposure and topical application bioassays we demonstrated that the fruit extract of P. lentiscus and its metabolites were toxic on L. botrana larvae. Extracts from leaves and bark showed no effect. Βioassay-guided fractionation of P. lentiscus fruit hexane extract led to the identification of its constituents with insecticidal properties on L. botrana larvae. Specifically, we have identified that the main contributor to the bioactivity of the hexane extract of P. lentiscus fruits is its major fraction, PLFHe2 (76.25%). Furthermore, we have found that PLFHe2 is a mixture of triglycerides and that the fatty acids responsible for the observed toxicity are oleic and linoleic acid.
Expression of Oxytocin/Neurophysin I and Oxytocinase in the Equine Conceptus from Day 8 to Day 21 Post-Ovulation
Authors: Diel de Amorim Mariana , Klein Claudia , Foster Robert , Dong Lynn , Lopez-Rodriguez Maria Fernanda , Card Claire
Published: March 2022

Leucyl and cystinyl aminopeptidase (LNPEP/oxytocinase) is an enzyme that metabolizes oxytocin in serum and tissues. The presence of oxytocin/neurophysin I (OXT), oxytocin and LNPEP and their relationship to other genes is unknown in the equine conceptus. Our objective was to characterize gene expression of LNPEP and OXT on D8, 10, 12, 14, 15, 16 and 21 conceptuses in relationship to other genes. Immunohistochemistry, western blot and liquid chromatography with tandem mass spectrometry (LC-MS/MS) were used for identification of oxytocin and LNPEP in D15, 16 and 18 conceptuses. LNPEP was increased at D15 compared to D10, was immunolocalized in the equine trophectoderm and endoderm, and protein was confirmed by LC-MS/MS. Maximal abundance of OXT was at D21, and lowest on D12 and D14, but no protein was identified. OXTR abundance was highest on D14 and D21. LNPEP was correlated with PTGFR and PTGES on D12 and D14–D15, and high expression of PTGES, PTGS2 was found on D14, D15 and D21; PTGFR was found on D8 and D12–21. LNPEP may have a role in prostaglandin regulation and conceptus fixation by decreasing the availability of oxytocin. Further investigation on the role embryonic LNPEP during pregnancy is warranted.
Development of an Ultrasound Technique to Evaluate the Popliteal Complex in the Horse
Authors: Møller-Jensen Merete , Blomquist Michaela Hansen , Mortensen Camilla Lamhauge , Olsson Isolde Katharina Christersdotter , Cuevas-Ramos Gabriel
Published: March 2022

The popliteal tendon and muscle are major stabilizers of the human and dog knee, more specifically the postero-lateral corner. Injury to this complex causes posterior knee pain, and it is generally associated with other injured structures such as the lateral collateral ligament, lateral meniscus, and/or the cranial cruciate ligament. The popliteal complex is poorly reported in the horse, and its specific function has not been determined. Nevertheless, it is likely that it is similar to that of other species, and that injury to it could have similar clinical repercussions. Lameness due to stifle pathology is a serious clinical entity in sport horses. One of the cardinal diagnostic tools in lameness exams is ultrasonography; however, a comprehensive technique to examine the popliteal complex (tendon and muscle) in the horse has not been reported. The objective of the study was to develop a systematic ultrasound technique of the equine popliteal complex that allows identification of the insertion and variations of the popliteal tendon (PopT), as well as examination of the popliteal muscle (PopM). Comparison between anatomical variants among horses was studied in order to identify possible significant differences. For this, 10 university teaching horses were used, hence 20 stifles were examined, several times. With the ultrasound technique presented here, the PopT and PopM are consistently examined. The developed technique allows reliable examination of the popliteal complex in the horse, and it could be included during standard ultrasound examination of equine stifle.
Morphology and Histology of the Orbital Region and Eye of the Asiatic Black Bear (Ursus thibetanus)—Similarities and Differences within the Caniformia Suborder
Authors: Paszta Wojciech , Goździewska-Harłajczuk Karolina , Klećkowska-Nawrot Joanna
Published: March 2022

In this study, we present first data concerning the morphological observations of the orbital region, eye tunics, upper and lower eyelids, superficial gland of the third eyelid with the third eyelid, and lacrimal gland in captive adult male Asiatic black bear. The following research methods were used in the work: the eyeball morphometry, the orbital region description, macroscopic description, morphometric and histological analysis of the eye tunics and selected the accessory organs of the eye (Fontana–Masson, hematoxylin & eosin (H&E), Methyl-green-pyronin Y (MGP Y), Movat pentachrome, and picro-Mallory trichrome) as well as histochemical examination (PAS, AB pH 1.0, AB pH 2.5, AB pH 2.5/PAS and HDI). The eyeball of the Asiatic black bear was a spherical shape, while the periorbita was funnel/conical-shaped and the eye socket was of the open type. The cornea was absent of the Bowman’s membrane similar to all domestic dogs and some wild dogs. There were palisades of Vogt in the corneal limbus epithelium similar to the Canidae. Degenerative choroidal tapetum lucidum similar to ranch mink (Mustelidae) has been found. The pupil was big and round in shape. The ciliary muscle, dilatator and sphincter muscle were well developed, similar to the pinnipeds. The lens was biconvex round, similar to the Canidae. The retina was composed similarly to the diurnal terrestrial carnivores. In both eyelids were observed very well-developed tarsal glands, ciliary glands and sebaceous glands. The orbital zone in the eyelids was characterized by lymphoid follicles, diffuse lymphocytes and specialized high endothelial venules. In the anterior palpebral margin of the upper eyelid, soft and short eyelashes were observed, while in the lower eyelids they were absent. The third eyelid was T-shaped and composed of the hyaline tissue, and it contained CALT, similar to that in Canidae. The superficial gland of the third eyelid was a multilobar alveolar branched complex with seromucous nature, while the lacrimal gland was also a multilobar acinar branched complex gland, but producing a mucous–serous secretion. The results of our research indicate that the features of the anatomy of the eye and orbital region in Asiatic black bear are also typical of the Ursidae family. Moreover, a detailed analysis of the morphological eye region may be useful in comparative studies and veterinary diagnostics in this bear species.
What Is in Your Shark Fin Soup? Probably an Endangered Shark Species and a Bit of Mercury
Authors: Choy Christina Pei Pei , Wainwright Benjamin J.
Published: March 2022

Shark fin soup, consumed by Asian communities throughout the world, is one of the principal drivers of the demand of shark fins. This near USD 1 billion global industry has contributed to a shark population declines of up to 70%. In an effort to arrest these declines, the trade in several species of sharks is regulated under the auspices of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). Despite this legal framework, the dried fins of trade-regulated sharks are frequently sold in markets and consumed in shark fin soup. Shark fins found in soups break down into a fibrous mass of ceratotrichia, meaning that identifying the species of sharks in the soup becomes impossible by visual methods. In this paper, we use DNA barcoding to identify the species of sharks found in bowls of shark fin soup collected in Singapore. The most common species identified in our samples was the blue shark (Prionace glauca), a species listed as Near Threatened on the International Union for Conservation of Nature (IUCN) Red List with a decreasing population, on which scientific data suggests catch limits should be imposed. We identified four other shark species that are listed on CITES Appendix II, and in total ten species that are assessed as Critically Endangered, Endangered or Vulnerable under the IUCN Red List of Threatened Species. Globally, the blue shark has been shown to contain levels of mercury that frequently exceed safe dose limits. Given the prevalence of this species in the examined soups and the global nature of the fin trade, it is extremely likely that consumers of shark fin soup will be exposed to unsafe levels of this neurotoxin.
The Second Case of Non-Mosaic Trisomy of Chromosome 26 with Homologous Fusion 26q;26q in the Horse
Authors: Ghosh Sharmila , Kjöllerström Josefina , Metcalfe Laurie , Reed Stephen , Juras Rytis , Raudsepp Terje
Published: March 2022

We present cytogenetic and genotyping analysis of a Thoroughbred foal with congenital neurologic disorders and its phenotypically normal dam. We show that the foal has non-mosaic trisomy for chromosome 26 (ECA26) but normal 2n = 64 diploid number because two copies of ECA26 form a metacentric derivative chromosome der(26q;26q). The dam has normal 64,XX karyotype indicating that der(26q;26q) in the foal originates from errors in parental meiosis or post-fertilization events. Genotyping ECA26 microsatellites in the foal and its dam suggests that trisomy ECA26 is likely of maternal origin and that der(26q;26q) resulted from Robertsonian fusion. We demonstrate that conventional and molecular cytogenetic approaches can accurately identify aneuploidy with a derivative chromosome but determining the mechanism and parental origin of the rearrangement requires genotyping with chromosome-specific polymorphic markers. Most curiously, this is the second case of trisomy ECA26 with der(26q;26q) in the horse, whereas all other equine autosomal trisomies are ‘traditional’ with three separate chromosomes. We discuss possible ECA26 instability as a contributing factor for the aberration and likely ECA26-specific genetic effects on the clinical phenotype. Finally, because ECA26 shares evolutionary homology with human chromosome 21, which trisomy causes Down syndrome, cytogenetic, molecular, and phenotypic similarities between trisomies ECA26 and HSA21 are discussed.
Evaluating ADHD Assessment for Dogs: A Replication Study
Authors: Csibra Barbara , Bunford Nóra , Gácsi Márta
Published: March 2022

The family dog, in its natural environment, exhibits neuropsychological deficits redolent of human psychiatric disorders, including behaviours similar to human Attention-Deficit/Hyperactivity Disorder (ADHD) symptoms. For dogs, Vas and colleagues developed a 13-item questionnaire to measure inattention and hyperactivity/impulsivity (Dog ARS; 2007). We re-assessed, in a large sample of dogs (N = 319), psychometric properties of the Dog ARS, to identify possible limitations as a basis for further development. We examined the cross-study stability of factor structure and 40-day temporal stability of item and subscale scores and compared owner-report with expert (dog trainer)-report (n = 86), paralleling human parent/teacher assessments. To identify ambiguous items, we administered a modified version (including “I don’t know” options, N = 520) to a different sample. We could replicate the factor structure with evidence of good internal consistency and test–retest reliability of both subscales. Agreement between owner and trainer ratings was fair (inattention) and moderate (hyperactivity/impulsivity). Three ambiguous items were identified. Overall, we claim that the Dog ARS is a reliable tool to assess ADHD-like behaviour in dogs, but in its current form, it is not suitable to detect diagnosable individuals, as it does not comprise items assessing functional impairment, and also, the inclusion of owner-expert ratings in the evaluation process would be necessary.
Benchmarking Based on Regularly Recorded Claw Health Data of Austrian Dairy Cattle for Implementation in the Cattle Data Network (RDV)
Authors: Kofler Johann , Suntinger Marlene , Mayerhofer Martin , Linke Kristina , Maurer Lorenz , Hund Alexandra , Fiedler Andrea , Duda Jürgen , Egger-Danner Christa
Published: March 2022

While benchmarking is already used for the assessment of performance gaps in cattle herd management and welfare concerns, its application to quantifying claw health performance is relatively new. The goal here was to establish a benchmarking system for claw health in Austrian dairy cattle. We used electronically registered claw health data of cows from 512 dairy herds documented by professional hoof trimmers, culling data from the same herds, and locomotion scores taken at regular milk performance testings in 99 dairy herds during 2020. Mean, median and the 10th, 25th, 75th, and 90th percentiles of the incidences of risk of lameness, 13 common claw lesions, and the annual culling risk directly related to claw and limb disorders were used as key performance indicators. Only validated data sets were used and participating trimmers and locomotion scorers had to pass interobserver reliability tests with weighted Cohen’s kappa values ≥ 0.61 indicating substantial interobserver agreement. This claw health benchmarking system is intended to be used henceforth in the transnational cattle data network (RDV) by all participating farmers and is also available for veterinarians and consultants, with the agreement of respective farmers.
Meat Fatty Acid Composition of Wild Boars Hunted in Romania in Relationship to Gender and Age-Class
Authors: Ciobanu Marius Mihai , Postolache Alina Narcisa , Lipşa Florin Daniel , Munteanu Mugurel , Rațu Roxana Nicoleta , Murariu Otilia Cristina , Boișteanu Paul Corneliu
Published: March 2022

The present study evaluates the influence of gender and age-class on the carcass traits, meat chemical composition, and fatty acid profiles of randomly hunt-harvested wild boars from Romania’s Frasin District hunting area, which is an important part of the stock density management strategy. Only 76 wild boars were chosen to participate in the experimental design from a total of 94 wild boars hunted over seven winter seasons, based on two main criteria: right carcass part intact and animal health status (suitable for human consumption) as determined by the veterinary health notice issued by the Food Safety Authority, with all wild boars being evaluated before slaughtering. After sanitary inspection and within the first 24 h postmortem, meat samples were taken in duplicate from the right side of wild boar carcasses (m. Biceps femoris). The aim of this study was to compare the carcass characteristics, chemical composition, and fatty acid profile of wild boar Biceps femoris muscle obtained from the right side of carcasses, as well as the effects of gender, age-class, and their interaction on these traits, in a context where venison is frequently regarded as organic food by consumers due to the natural habitat in which wild animals live. The age of the animals had an effect on the postmortem undressed warm and cold carcass weights (p ≤ 0.05). Considering the proximal chemical composition, only the intramuscular fat (IMF) content was higher in adult meat samples compared to young animals. C18:1 n-9 was the most abundant fatty acid in wild boar meat, followed by C16:0, C18:2 n-6, and C18:0. For IMF content, gender, age class, and their interaction influenced mainly MUFA fatty acids, in particular C16:1 n-9, C20:1 n-9 and C22:1 n-9, and also PUFA fatty acids C18:3 n-6, C20:3 n-3, C20:4 n-6, C20:5 n-3, C22:4 n-6, and C22:5 n-6. The appropriate amounts of individual fatty acids in the muscles of the wild boars translate into a ratio of P/S acids that promotes health as a bio-alternative resource of meat.
Deep Reinforcement Learning-Based DQN Agent Algorithm for Visual Object Tracking in a Virtual Environmental Simulation
Authors: Park Jin-Hyeok , Farkhodov Khurshedjon , Lee Suk-Hwan , Kwon Ki-Ryong
Published: March 2022

The complexity of object tracking models among hardware applications has become a more in-demand task to accomplish with multifunctional algorithm skills in various indeterminable environment tracking conditions. Experimenting with the virtual realistic simulator brings new dependencies and requirements, which may cause problems while experimenting with runtime processing. The goal of this paper is to present an object tracking framework that differs from the most advanced tracking models by experimenting with virtual environment simulation (Aerial Informatics and Robotics Simulation—AirSim, City Environ) using one of the Deep Reinforcement Learning Models named as Deep Q-Learning algorithms. Our proposed network examines the environment using a deep reinforcement learning model to regulate activities in the virtual simulation environment and utilizes sequential pictures from the realistic VCE (Virtual City Environ) model as inputs. Subsequently, the deep reinforcement network model was pretrained using multiple sequential training image sets and fine-tuned for adaptability during runtime tracking. The experimental results were outstanding in terms of speed and accuracy. Moreover, we were unable to identify any results that could be compared to the state-of-the-art methods that use deep network-based trackers in runtime simulation platforms, since this testing experiment was conducted on the two public datasets VisDrone2019 and OTB-100, and achieved better performance among compared conventional methods.
Cloudformer: Supplementary Aggregation Feature and Mask-Classification Network for Cloud Detection
Authors: Zhang Zheng , Xu Zhiwei , Liu Chang’an , Tian Qing , Wang Yanping
Published: March 2022

Cloud detection is an important step in the processing of optical satellite remote-sensing data. In recent years, deep learning methods have achieved excellent results in cloud detection tasks. However, most of the current models have difficulties to accurately classify similar objects (e.g., clouds and snow) and to accurately detect clouds that occupy a few pixels in an image. To solve these problems, a cloud-detection framework (Cloudformer) combining CNN and Transformer is being proposed to achieve high-precision cloud detection in optical remote-sensing images. The framework achieves accurate detection of thin and small clouds using a pyramidal structure encoder. It also achieves accurate classification of similar objects using a dual-path decoder structure of CNN and Transformer, reducing the rate of missed detections and false alarms. In addition, since the Transformer model lacks the perception of location information, an asynchronous position-encoding method is being proposed to enhance the position information of the data entering the Transformer module and to optimize the detection results. Cloudformer is experimented on two datasets, AIR-CD and 38-Cloud, and the results show that it has state-of-the-art performance.
Malaysia PM10 Air Quality Time Series Clustering Based on Dynamic Time Warping
Authors: Suris Fatin Nur Afiqah , Bakar Mohd Aftar Abu , Ariff Noratiqah Mohd , Mohd Nadzir Mohd Shahrul , Ibrahim Kamarulzaman
Published: March 2022

Air quality monitoring is important in the management of the environment and pollution. In this study, time series of PM10 from air quality monitoring stations in Malaysia were clustered based on similarity in terms of time series patterns. The identified clusters were analyzed to gain meaningful information regarding air quality patterns in Malaysia and to identify characterization for each cluster. PM10 time series data from 5 July 2017 to 31 January 2019, obtained from the Malaysian Department of Environment and Dynamic Time Warping as the dissimilarity measure were used in this study. At the same time, k-Means, Partitioning Around Medoid, agglomerative hierarchical clustering, and Fuzzy k-Means were the algorithms used for clustering. The results portray that the categories and activities of locations of the monitoring stations do not directly influence the pattern of the PM10 values, instead, the clusters formed are mainly influenced by the region and geographical area of the locations.
Analysis of PM2.5 and Meteorological Variables Using Enhanced Geospatial Techniques in Developing Countries: A Case Study of Cartagena de Indias City (Colombia)
Authors: Aldegunde José Antonio Álvarez , Sánchez Adrián Fernández , Saba Manuel , Bolaños Edgar Quiñones , Palenque José Úbeda
Published: March 2022

The dispersion of air pollutants and the spatial representation of meteorological variables are subject to complex atmospheric local parameters. To reduce the impact of particulate matter (PM2.5) on human health, it is of great significance to know its concentration at high spatial resolution. In order to monitor its effects on an exposed population, geostatistical analysis offers great potential to obtain high-quality spatial representation mapping of PM2.5 and meteorological variables. The purpose of this study was to define the optimal spatial representation of PM2.5, relative humidity, temperature and wind speed in the urban district in Cartagena, Colombia. The lack of data due to the scarcity of stations called for an ad hoc methodology, which included the interpolation implementing an ordinary kriging (OK) model, which was fed by data obtained through the inverse distance weighting (IDW) model. To consider wind effects, empirical Bayesian kriging regression prediction (EBK) was implemented. The application of these interpolation methods clarified the areas across the city that exceed the recommended limits of PM2.5 concentrations (Zona Franca, Base Naval and Centro district), and described in a continuous way, on the surface, three main weather variables. Positive correlations were obtained for relative humidity (R2 of 0.47), wind speed (R2 of 0.59) and temperature (R2 of 0.64).
Synthesis and Characterization of Atmospherically Relevant Hydroxy Hydroperoxides
Authors: Mettke Peter , Mutzel Anke , Böge Olaf , Herrmann Hartmut
Published: March 2022

Hydroxy hydroperoxides are formed upon OH oxidation of volatile organic compounds in the atmosphere and may contribute to secondary organic aerosol growth and aqueous phase chemistry after phase transfer to particles. Although the detection methods for oxidized volatile organic compounds improved much over the past decades, the limited availability of synthetic standards for atmospherically relevant hydroxy hydroperoxides prevented comprehensive investigations for the most part. Here, we present a straightforward improved synthetic access to isoprene-derived hydroxy hydroperoxides, i.e., 1,2-ISOPOOH and 4,3-ISOPOOH. Furthermore, we present the first successful synthesis of an α-pinene derived hydroxy hydroperoxide. All products were identified by 1H, 13C NMR spectroscopy for structure elucidation, additional 2D NMR experiments were performed. Furthermore, gas-phase FTIR- and UV/VIS spectra are presented for the first time. Using the measured absorption cross section, the atmospheric photolysis rate of up to 2.1 × 10−3 s−1 was calculated for 1,2-ISOPOOH. Moreover, we present the investigation of synthesized hydroxy hydroperoxides in an aerosol chamber study by online MS techniques, namely PTR-ToFMS and (NO3−)-CI-APi-ToFMS. Fragmentation patterns recorded during these investigations are presented as well. For the (NO3−)-CI-APi-ToFMS, a calibration factor for 1,2-ISOPOOH was calculated as 4.44 × 10−5 ncps·ppbv−1 and a LOD (3σ, 1 min average) = 0.70 ppbv.
Impact of Pulsed Electric Field Pre-Treatment on the Isoflavone Profile of Soymilk
Authors: Morales-de la Peña Mariana , Welti-Chanes Jorge , Martín-Belloso Olga
Published: March 2022

In this study, pulsed electric fields (PEFs) were evaluated as extraction-aiding technology during soymilk manufacturing to improve its isoflavone profile. Low-intensity PEFs were applied at different processing conditions in two stages of the soymilk extraction process, hydrated soybeans (HSB) and soybean slurry (SBS), with the soymilk extracted from the conventional process as control (CSM). Overall, resultant soymilk samples from PEF-HSB and PEF-SBS presented lower concentrations of glucosides isoflavones and greater aglycone content than those in CSM. In contrast to genistin (Gin) and daidzin (Din), which decreased around 18.5–52.6% and 10.9–54.6%, respectively, an increase in genistein (Ge, 12.3–64.4%) and daidzein (Da, 9–55.8%) was observed. The total isoflavone content (TIC) of most soymilk samples prepared from PEF-HSB was lower than that of the CSM. Conversely, when PEF-SBS was used, the TIC of resultant soymilk was not significantly affected or slightly decreased. However, PEF treated HSB at 10 kVcm−1/100 pulses and SBS at 6 kVcm−1/10 pulses led to a significant augment in TIC, of up to 109 ± 2.39 and 110 ± 1.26 μg/g, respectively, in the extracted soymilk samples. These results indicated that low-intensity PEF is a potential technology that could be implemented during soymilk manufacturing processing to modify the isoflavone profile and content of soymilk, mainly increasing its aglycone concentration.
Effectiveness of Platform-Based Robot-Assisted Rehabilitation for Musculoskeletal or Neurologic Injuries: A Systematic Review
Authors: Payedimarri Anil Babu , Ratti Matteo , Rescinito Riccardo , Vanhaecht Kris , Panella Massimiliano
Published: March 2022

During the last ten years the use of robotic-assisted rehabilitation has increased significantly. Compared with traditional care, robotic rehabilitation has several potential advantages. Platform-based robotic rehabilitation can help patients recover from musculoskeletal and neurological conditions. Evidence on how platform-based robotic technologies can positively impact on disability recovery is still lacking, and it is unclear which intervention is most effective in individual cases. This systematic review aims to evaluate the effectiveness of platform-based robotic rehabilitation for individuals with musculoskeletal or neurological injuries. Thirty-eight studies met the inclusion criteria and evaluated the efficacy of platform-based rehabilitation robots. Our findings showed that rehabilitation with platform-based robots produced some encouraging results. Among the platform-based robots studied, the VR-based Rutgers Ankle and the Hunova were found to be the most effective robots for the rehabilitation of patients with neurological conditions (stroke, spinal cord injury, Parkinson’s disease) and various musculoskeletal ankle injuries. Our results were drawn mainly from studies with low-level evidence, and we think that our conclusions should be taken with caution to some extent and that further studies are needed to better evaluate the effectiveness of platform-based robotic rehabilitation devices.
3D Printing in Solid Dosage Forms and Organ-on-Chip Applications
Authors: Kassem Tarek , Sarkar Tanoy , Nguyen Trieu , Saha Dipongkor , Ahsan Fakhrul
Published: March 2022

3D printing (3DP) can serve not only as an excellent platform for producing solid dosage forms tailored to individualized dosing regimens but can also be used as a tool for creating a suitable 3D model for drug screening, sensing, testing and organ-on-chip applications. Several new technologies have been developed to convert the conventional dosing regimen into personalized medicine for the past decade. With the approval of Spritam, the first pharmaceutical formulation produced by 3DP technology, this technology has caught the attention of pharmaceutical researchers worldwide. Consistent efforts are being made to improvise the process and mitigate other shortcomings such as restricted excipient choice, time constraints, industrial production constraints, and overall cost. The objective of this review is to provide an overview of the 3DP process, its types, types of material used, and the pros and cons of each technique in the application of not only creating solid dosage forms but also producing a 3D model for sensing, testing, and screening of the substances. The application of producing a model for the biosensing and screening of drugs besides the creation of the drug itself, offers a complete loop of application for 3DP in pharmaceutics.
Patient Experience of Flunarizine for Vestibular Migraine: Single Centre Observational Study
Authors: Rashid Sk Mamun Ur , Sumaria Sheetal , Koohi Nehzat , Arshad Qadeer , Kaski Diego
Published: March 2022

Vestibular migraine (VM) is a leading cause of episodic vertigo, affecting up to 1% of the general population. Despite established diagnostic criteria, there is currently no evidence-based approach for acute treatment of VM, with treatment recommendations generally extrapolated from studies on classical migraine headache. Several small-scale studies have identified flunarizine as a potentially effective prophylactic medication in VM. We conducted a single-centre observational service evaluation study exploring patient experiences of preventative medications over a 28-month period, including flunarizine, for control of VM symptoms. To compare patient experience of flunarizine with other medications, data from patients taking flunarizine were separately analysed. A total of 90% of VM patients taking flunarizine reported symptomatic improvement, compared to only 32% of patients on other medications. Whilst 50% of patients on flunarizine reported side effects. these were not deemed to outweigh the clinical benefits, with most patients deciding to continue treatment. Our data supports the use of flunarizine in VM.
Positive and Negative Symptoms Are Associated with Distinct Effects on Predictive Saccades
Authors: Smith Eleanor S. , Crawford Trevor J.
Published: March 2022

The predictive saccade task is a motor learning paradigm requiring saccades to track a visual target moving in a predictable pattern. Previous research has explored extensively anti-saccade deficits observed across psychosis, but less is known about predictive saccade-related mechanisms. The dataset analysed came from the studies of Crawford et al, published in 1995, where neuroleptically medicated schizophrenia and bipolar affective disorder patients were compared with non-medicated patients and control participants using a predictive saccade paradigm. The participant groups consisted of medicated schizophrenia patients (n = 40), non-medicated schizophrenia patients (n = 18), medicated bipolar disorder patients (n = 14), non-medicated bipolar disorder patients (n = 18), and controls (n = 31). The current analyses explore relationships between predictive saccades and symptomatology, and the potential interaction of medication. Analyses revealed that the schizophrenia and bipolar disorder diagnostic categories are indistinguishable in patterns of predictive control across several saccadic parameters, supporting a dimensional hypothesis. Once collapsed into predominantly high-/low- negative/positive symptoms, regardless of diagnosis, differences were revealed, with significant hypometria and lower gain in those with more negative symptoms. This illustrates how the presentation of the deficits is homogeneous across diagnosis, but heterogeneous when surveyed by symptomatology; attesting that a diagnostic label is less informative than symptomatology when exploring predictive saccades.