bluuebunny/crossref_metadata_2025 huggingface dataset:
Long-Term Follow-Up Outcomes in Women with In Situ/Microinvasive Adenocarcinoma of the Uterine Cervix Undergoing Conservative Treatment—Cervical Adenocarcinoma Study Group Italian Society of Colposcopy and Cervico-Vaginal Pathology
Authors: Giannella Luca , Delli Carpini Giovanni , Di Giuseppe Jacopo , Grelloni Camilla , Bogani Giorgio , Dri Marco , Sopracordevole Francesco , Clemente Nicolò , Giorda Giorgio , De Vincenzo Rosa , Evangelista Maria Teresa , Gardella Barbara , Dominoni Mattia , Monti Ermelinda , Alessi Chiara , Alessandrini Lara , Guerriero Angela , Pagan Alessio , Caretto Marta , Ghelardi Alessandro , Amadori Andrea , Origoni Massimo , Barbero Maggiorino , Raspagliesi Francesco , Simoncini Tommaso , Vercellini Paolo , Spinillo Arsenio , Scambia Giovanni , Ciavattini Andrea
Published: March 2024

Objective: The present study aimed to assess long-term follow-up outcomes in women with in situ/microinvasive adenocarcinoma (AC) of the uterine cervix treated conservatively. Methods: Retrospective multi-institutional study including women with early glandular lesions and 5-year follow-up undergoing fertility-sparing treatment. Independent variables associated with recurrence were evaluated. Logistic regression analysis and Kaplan–Meier survival analysis with Logrank test were performed. Results: Of 269 women diagnosed with in situ/microinvasive AC, 127 participants underwent conservative treatment. During follow-up, recurrences were found in nine women (7.1%). The only factor associated with recurrence during follow-up was positive high-risk Human Papillomavirus (hr-HPV) testing (odds ratio 6.21, confidence interval 1.47–26.08, p = 0.012). HPV positivity in follow-up showed a recurrence rate of 21.7% against 3.8% in patients who were HPV-negative (p = 0.002, Logrank test). Among women with negative high-risk HPV tests in follow-up, recurrences occurred in 20.0% of non-usual-type histology vs. 2.1% of usual-type cases (p = 0.005). Conclusion: HPV testing in follow-up is of pivotal importance in women with early glandular lesions undergoing conservative treatment, given its recurrence predictive value. However, women who are high-risk HPV-negative in follow-up with non-usual-type histopathology may represent a sub-population at increased risk of recurrences. Further studies should confirm these findings.
Everolimus Mitigates the Risk of Hepatocellular Carcinoma Recurrence after Liver Transplantation
Authors: De Simone Paolo , Precisi Arianna , Lai Quirino , Ducci Juri , Campani Daniela , Marchetti Piero , Gitto Stefano
Published: March 2024

To obtain long-term data on the use of everolimus in patients who underwent liver transplantation for hepatocellular carcinoma, we conducted a retrospective, single-center analysis of adult recipients transplanted between 2013 and 2021. Patients on everolimus-incorporating immunosuppression were matched with those on tacrolimus using an inverse probability of treatment weighting methodology. Two propensity-matched groups of patients were thus compared: 233 (45.6%) receiving everolimus versus 278 (54.4%) on tacrolimus. At a median (interquartile range) follow-up of 4.4 (3.8) years after transplantation, everolimus patients showed a reduced risk of recurrence versus tacrolimus (7.7% versus 16.9%; RR = 0.45; p = 0.002). At multivariable analysis, microvascular infiltration (HR = 1.22; p < 0.04) and a higher tumor grading (HR = 1.27; p < 0.04) were associated with higher recurrence rate while being within Milan criteria at transplant (HR = 0.56; p < 0.001), a successful pre-transplant downstaging (HR = 0.63; p = 0.01) and use of everolimus (HR = 0.46; p < 0.001) had a positive impact on the risk of post-transplant recurrence. EVR patients with earlier drug introduction (≤30 days; p < 0.001), longer treatment duration (p < 0.001), and higher drug exposure (≥5.9 ng/mL; p < 0.001) showed lower recurrence rates versus TAC. Based on our experience, everolimus provides a reduction in the relative risk of hepatocellular carcinoma recurrence, especially for advanced-stage patients and those with earlier drug administration, higher drug exposure, and longer time on treatment. These data advocate for early everolimus introduction after liver transplantation to reduce the attrition rate consequent to chronic immunosuppression.
The Impact of Grain Growth on the Functional Properties in Room-Temperature Powder Aerosol Deposited Free-Standing (Ba,Ca)(Zr,Ti)O3 Thick Films
Authors: Maier Juliana G. , Fuggerer Tim , Urushihara Daisuke , Martin Alexander , Khansur Neamul H. , Kakimoto Ken-ichi , Webber Kyle G.
Published: March 2024

This study investigates the development of freestanding thick films (FSFs) of lead-free (Ba,Ca)(Zr,Ti)O3 and the role of grain growth on the electromechanical response. During deposition, room temperature powder aerosol deposition rapidly produces thick films with a nano-grain structure that limits the electromechanical properties. In this study, the films are removed from the substrate using a sacrificial buffering layer to avoid thermal treatment and allow for an initial as-processed state. Following this, FSFs were thermally treated at various annealing temperatures from 800 °C to 1400 °C to induce grain growth, which was characterized with scanning and transmission electron microscopy. X-ray diffraction revealed an increase in the crystallite size consistent with an increase in grain size and a decrease in internal residual stress. The temperature-dependent dielectric behavior and the large-field ferroelectric response were also characterized, revealing significant differences of the FSFs from the bulk properties.
Statistical Mechanics of Electrowetting
Authors: Louge Michel Y. , Wang Yujie
Published: March 2024

We derive the ab initio equilibrium statistical mechanics of the gas–liquid–solid contact angle on planar periodic, monodisperse, textured surfaces subject to electrowetting. To that end, we extend an earlier theory that predicts the advance or recession of the contact line amount to distinct first-order phase transitions of the filling state in the ensemble of nearby surface cavities. Upon calculating the individual capacitance of a cavity subject to the influence of its near neighbors, we show how hysteresis, which is manifested by different advancing and receding contact angles, is affected by electrowetting. The analysis reveals nine distinct regimes characterizing contact angle behavior, three of which arise only when a voltage is applied to the conductive liquid drop. As the square voltage is progressively increased, the theory elucidates how the drop occasionally undergoes regime transitions triggering jumps in the contact angle, possibly changing its hysteresis, or saturating it at a value weakly dependent on further voltage growth. To illustrate these phenomena and validate the theory, we confront its predictions with four data sets. A benefit of the theory is that it forsakes trial and error when designing textured surfaces with specific contact angle behavior.
Effect of the Addition of Elderberry Waste to Sawdust on the Process of Pelletization and the Quality of Fuel Pellets
Authors: Obidziński Sławomir , Cwalina Paweł , Kowczyk-Sadowy Małgorzata , Sienkiewicz Aneta , Krasowska Małgorzata , Szyszlak-Bargłowicz Joanna , Zając Grzegorz , Kryński Rafał
Published: March 2024

This paper presents the results of a study on the process of the pelletization of pine sawdust with the addition of herbaceous waste from elderberry, in the working system of a pellet press with a flat matrix, in the context of producing fuel pellets. Based on the research, the impact of the addition of herbal waste in the form of elderberry waste on the granulation process of pine sawdust and the assessment of the quality of the obtained pellets were determined. The addition of herbaceous waste from elderberry to pine sawdust had a beneficial effect on the kinetic durability of the obtained fuel pellets, with an increase of up to approximately 1.3% (from 98.03 to 99.31%). Based on the obtained results, it can be concluded that the mechanical strength of all the tested pellets is higher than 97.5%, which is consistent with the ISO 17225-1:2021-11 standard. The bulk density of pellets with the addition of herbaceous waste from elderberry increased (from 649.34 to 658.50 kg∙m−3) as did their density (from 1231.38 to 1263.90 kg∙m−3). The addition of herbaceous waste from elderberry in amounts ranging from 10% to 20% did not have a significant effect on the power requirements of the pelletizer, which decreased compared to the pelletization process of pure pine sawdust. The percentage of this decrease compared to the pelleting process with pure pine sawdust was approximately 10%. The addition of herbaceous waste from elderberry to pine sawdust slightly reduces the energy value (i.e., the heat of combustion and the calorific value) of the obtained pellets. The addition of 30% elderberry waste resulted in a decrease in the heat of combustion from 20.27 to 19.96 MJ·kgd.m.−1, while the calorific value of the pellets decreased from 19.98 to 18.69 MJ·kgd.m.−1 compared to pine sawdust pellets. Hence, adding herbaceous waste from elderberry seems to be a good way of managing large amounts of waste of this kind generated in herbal processing plants. This method of waste management opens new perspectives towards more sustainable and economically effective energy production.
Tilt Angle Effect on Natural Convection Heat Transfer from an Inclined Array of Square Cylinders
Authors: Al-Suhaibani Zeyad , Ali Mohamed , Almuzaiqer Redhwan
Published: March 2024

Natural convection heat transfer from an inclined array of square cross-section cylinders is experimentally studied. Three cylinders of side length D = 0.02 m and 1 m length are used to form the inclined array. Three-cylinder axis inclination angles to the horizontal of 30°, 45°, and 60° are considered. The cylinders are heated using an internal heating element of constant heat flux. Surface temperatures are measured along the three surfaces of each cylinder; upper, lower, and the front side at nine points spaced evenly by 10 cm. The local circumference average temperature is obtained at each point. Four center-to-center distances to side length S/D = 1.25, 1.75, 2.25, and 2.75 are used. Local Nusselt numbers and the modified Rayleigh numbers are obtained for each cylinder at each circumference averaged temperature point. Results show that the lower cylinder heat transfer is least affected by the array compared to that of its single one followed by the middle and the upper cylinder for all S/D and the inclination angles used. The Nusselt number is degraded from that of the single cylinder at a small S/D and that degradation decreases as the S/D increases for all angles. It is observed that the Nusselt number enhances at the small cylinder axis tilt angle of 30°, followed by 45°, and 60°, especially at high modified Rayleigh numbers. New novel general empirical correlations for Nusselt numbers are obtained for each S/D using the cylinder axis tilt angle, the modified Rayleigh numbers, and the cylinder number in the array as parameters. A new general correlation is obtained for the array using the modified Rayleigh number, S/D, the cylinder axis tilt angle, and the cylinder sequential order number as parameters. These new correlations will help any engineering applications using such a configuration of the array.
Development of a Hydrogen Fuel Cell Prototype Vehicle Supported by Artificial Intelligence for Green Urban Transport
Authors: Kun Krisztián , Szabó Lóránt , Varga Erika , Kis Dávid István
Published: March 2024

In the automotive sector, the zero emissions area has been dominated by battery electric vehicles. However, prospective users cite charging times, large batteries, and the deployment of charging stations as a counter-argument. Hydrogen will offer a solution to these areas, in the future. This research focuses on the development of a prototype three-wheeled vehicle that is named Neumann H2. It integrates state-of-the-art energy storage systems, demonstrating the benefits of solar-, battery-, and hydrogen-powered drives. Of crucial importance for the R&D platform is the system’s ability to record its internal states in a time-synchronous format, providing valuable data for researchers and developers. Given that the platform is equipped with the ROS2 Open-Source interface, the data are recorded in a standardized format. Energy management is supported by artificial intelligence of the “Reinforcement Learning” type, which selects the optimal energy source for operation based on different layers of high-fidelity maps. In addition to powertrain control, the vehicle also uses artificial intelligence to detect the environment. The vehicle’s environment-sensing system is essentially designed to detect, distinguish, and select environmental elements through image segmentation using camera images and then to provide feedback to the user via displays.
Study on the Influence of the Application of Phase Change Material on Residential Energy Consumption in Cold Regions of China
Authors: Wang Chen , Shao Yu , Zhao Bolun , Chen Yang , Yu Jiahui , Guo Haibo
Published: March 2024

As the impact of climate change intensifies, meeting the energy demand of buildings in China’s cold regions is becoming increasingly challenging, particularly in terms of cooling energy consumption. The effectiveness of integrating phase change material (PCM) into building envelopes for energy saving in China’s cold regions is unclear. The aim of this study is to assess the effectiveness of PCM integration in building enclosures for energy efficiency in these regions. The research monitored and recorded indoor temperature data from typical residential cases from May to September. This measured data was then used to validate the accuracy of EnergyPlus22-1 software simulation models. Subsequently, the calibrated model was utilized to conduct a comparative analysis on the effects of PCM on indoor temperatures and cooling energy consumption across these regions. The results of these comparative analyses indicated that PCM can alleviate indoor overheating to varying degrees in severe cold regions of China. Focusing on north-facing bedrooms, applying PCMs reduced the duration of overheating in non-air-conditioned buildings in severe cold regions of China by 136 h (Yichun), 340 h (Harbin), 356 h (Shenyang), and 153 h (Dalian). In terms of cooling energy consumption, the energy saved by applying PCMs ranged from 1.48 to 13.83 kWh/m2. These results emphasize that the performance of PCM varies with climate change, with the most significant energy-saving effects observed in severe cold regions. In north-facing bedrooms in Harbin, the energy-saving rate was as high as 60.30%. Based on these results, the study offers guidance and recommendations for feasible passive energy-saving strategies for buildings in severe cold and cold regions of China in the face of climate change. Additionally, it provides practical guidance for applying PCMs in different climatic zones in China.
Assessing the Performance of Handheld Laser Scanning for Individual Tree Mapping in an Urban Area
Authors: Yang Jinming , Yuan Wenwen , Lu Huicui , Liu Yuehan , Wang Yongkang , Sun Letong , Li Shimei , Li Haifang
Published: March 2024

Precise individual tree or sample-based inventories derived from 3D point cloud data of mobile laser scanning can improve our comprehensive understanding of the structure, function, resilience, biodiversity, and ecosystem services of urban forests. This study assessed the performance of a handheld laser scanning system (HLS) for the extraction of tree position, diameter at breast height (DBH), and tree height (H) in an urban area. A total of 2083 trees of 13 species from 34 plots were analyzed. The results showed that the registration of tree positions using ground control points (GCPs) demonstrated high accuracy, with errors consistently below 0.4 m, except for a few instances. The extraction accuracy of DBH for all trees and individual species remained consistently high, with a total root mean square error (RMSE) of 2.06 cm (6.89%) and a bias of 0.62 cm (2.07%). Notably, broad-leaved trees outperformed coniferous trees, with RMSE and bias values of 1.86 cm (6%) and 0.76 cm (2.46%), respectively, compared to 2.54 cm (9.46%) and 0.23 cm (0.84%), respectively. The accuracy of H extraction varied significantly among different species, with R2 values ranging from 0.65 to 0.92. Generally, both DBH and H were underestimated compared to ground measurements. Linear mixed-effects models (LMEs) were applied to evaluate factors affecting the performance of HLS with the plot as a random factor. LME analysis revealed that plant type and terrain significantly influenced the accuracy of DBH and H derived from HLS data, while other fixed factors such as plot area, tree density, and trajectory length showed no significance. With a large sample size, we concluded that the HLS demonstrated sufficient accuracy in extracting individual tree parameters in urban forests.
Effects of Exercise Intensity Differences in Forest Therapy Programs on Immunoglobulin A and Dehydroepiandrosterone Levels in Older Adults
Authors: Shin Min-Ja , Kim Jaeuk U. , You Jin-Hee , Shin Won-Sop
Published: March 2024

The purpose of this study was to examine the effects of exercise intensity and the duration of an exercise-based forest therapy program on physiological changes in older adults. The forest therapy program consisted of 20 sessions over 10 weeks. Forty-one older adults aged 65 years and older were divided into no treatment (daily activity group) and experimental (forest therapy) groups. The experimental group was further divided into the medium-intensity forest exercise group and the high-intensity forest exercise group to analyze physiological changes according to exercise intensity and duration. The physiological changes were analyzed by collecting saliva and measuring changes in the levels of immunoglobulin A and dehydroepiandrosterone, a hormone used to predict aging. Changes within the daily activity group and the forest therapy group after their respective exercises were analyzed using paired t-tests. Saliva testing was performed before and in weeks 5 and 10 of the program and analyzed using a repeated measures analysis of variance to assess the effects of the forest therapy on the medium-intensity forest exercise group and the high-intensity forest exercise group based on the duration of exercise. As a result of the study, a significant increase in immunoglobulin A was observed in the medium-intensity forest exercise group. The daily activity group and the high-intensity forest exercise group showed an increase, but there was very little change and no significance. Changes in dehydroepiandrosterone decreased in the daily activity group and significantly increased in the moderate-intensity forest exercise group at 5 and 10 weeks, showing that regular moderate-intensity forest exercise has an effect on dehydroepiandrosterone. The high-intensity forest exercise group improved over time, but no significant level of change was observed. This study shows that forest exercise has a beneficial effect on immunoglobulin A and dehydroepiandrosterone levels in older adults.
A Comparative Study between Beeswax and Glycerol Monostearate for Food-Grade Oleogels
Authors: Malvano Francesca , Albanese Donatella , Cinquanta Luciano , Liparoti Sara , Marra Francesco
Published: March 2024

With the aim to produce solid fats with a high percentage of unsaturated fatty acids, oleogels based on olive and peanut oil with different concentrations of beeswax (BW) and glycerol monostearate (GMS) as oleogelators were studied and compared. The critical oleogelator concentration for both BW and GMS was 3%. Thermal properties of the developed GMS-based oleogels pointed to a polymorphic structure, confirmed by the presence of two exothermic and endothermic peaks. All developed oleogels released less than 4% of oil, highlighting their high oil binding capacity. A morphology evaluation of oleogels showed platelet-like crystals, characterized by a cross-sectional length of 50 μm in BW-based oleogels and irregular clusters of needle-like crystals with a higher diameter in GMS-based oleogels. BW-based oleogels showed a solid fat content ranging from 1.16% to 2.27%, and no solid fat content was found at 37 °C. GMS-based oleogels reached slightly higher values of SFC that ranged from 1.58% to 2.97% at 25 °C and from 1.00% to 1.75% at 37 °C. Olive oil-based oleogels with GMS showed higher firmness compared with BW-based ones. The stronger structure network in olive oil/GMS-based oleogels provided a real physical barrier to oxidants, showing a high oxidation stability.
Interaction Effects of FTO and MC4R Polymorphisms on Total Body Weight Loss, Post-Surgery Weight, and Post-Body Mass Index after Bariatric Surgery
Authors: Perez-Luque Elva , Daza-Hernandez Edgar S. , Figueroa-Vega Nicte , Cardona-Alvarado Monica I. , Muñoz-Montes Norberto , Martinez-Cordero Claudia
Published: March 2024

Bariatric surgery (BS) is considered the most effective intervention for patients with severe obesity and is used to maintain long-term weight loss and glycemic control. The aim of this study was to analyze the effects of genotypes and haplotypes of the fat mass and obesity-associated (FTO) and melanocortin 4 receptor (MC4R) genes on total body weight loss (TBWL), post-surgery weight, and post-BMI after bariatric surgery. We retrospectively selected 101 patients from Bajio High Specialty Regional Hospital, León Guanajuato, México, who underwent Roux-en-Y gastric bypass (RYGB) to determine their body mass index (BMI), blood pressure, biochemical characteristics, and comorbidities. Post-surgery, patients were referred for registered anthropometry and blood pressure. Glucose, lipid and hepatic profiles, and insulin, leptin, and ghrelin levels were measured, and rs9939609, rs9930506, and rs1421085 FTO and rs17782313 MC4R polymorphisms were genotyped. Six (4–8) years after BS, post-surgery weight was greater in carriers of the rs9939609 and rs1421085 risk genotypes. TBWL was lower for the rs9930506 and rs1421085 risk genotypes. Insulin and HOMA-IR were greater in patients with the three FTO polymorphisms. There were significant interaction effects of the rs9930506 and rs1421085 FTO risk genotypes on weight and BMI in response to BS. No association was found with the MC4R polymorphism. The genotypes and haplotypes of the FTO gene influence post-surgery weight, TBWL, insulin levels, and HOMA-IR.
Refugees and Representation: Introduction—The Mimesis of Diaspora
Authors: Newton Adam Zachary
Published: March 2024

In keeping with the title we have chosen for this follow-up volume to the Special Issue “Ethics and Literary Practice I”, we frame our introduction and summary of the essays collected here with a brief archaeology of modern literary realism at its conjoined genesis in classical Greece and the ancient Near East; such contextualization serves as a prescient backdrop for the varied focus, across a compilation of thirteen articles, on refugees and their representation [...]
The Identification of Cucumber TDC Genes and Analyses of Their Expression and Functions under Abiotic Stress Conditions
Authors: Zhang Yiqiu , Li Qiuxia , Liu Yu , Wan Shubei , Li Shuzhen
Published: March 2024

Melatonin is a crucial regulator of plant growth and development as well as stress tolerance. However, we only have a limited understanding of the functions of endogenous melatonin. Tryptophan decarboxylase (TDC) serves as the initial rate-limiting enzyme in the melatonin synthesis pathway. To date, no cucumber TDC gene has been cloned and characterized. In this study, we identified two TDC genes (CsTDC1 and CsTDC2) in the cucumber genome. The subcellular localization analysis indicated that CsTDC1 and CsTDC2 are predominantly localized in the cytoplasm and plasma membrane. Tissue-specific expression analyses revealed that CsTDC1 and CsTDC2 are expressed in both vegetative and reproductive organs. Many cis-elements related to stress, hormone, and light responses as well as development were identified in the CsTDC promoter regions. Furthermore, the expression of CsTDC1 and CsTDC2 was strongly induced by treatments with various abiotic stresses and exogenous hormones. The transient overexpression of CsTDC1 and CsTDC2 in tobacco leaves resulted in increases in the TDC activity and melatonin content, along with improved tolerance of tobacco leaves to salt, drought, and low-temperature stresses. Notably, the overexpression of CsTDC2 had a more pronounced effect than the overexpression of CsTDC1. Accordingly, both CsTDC genes, but especially CsTDC2, may be important for regulating cucumber growth, development, and stress tolerance. The study findings provide a theoretical and experimental basis for future functional analyses of endogenous melatonin in cucumber.
Planning Individual and Population-Based Interventions in Global Health: Applying the DEA-A Framework to Promote Behavioral, Emotional, and/or Cognitive Change among Stakeholders
Authors: Broc Guillaume , Fassier Jean Baptiste , Raffard Stéphane , Lareyre Olivier
Published: March 2024

Addressing health challenges that impact human well-being requires a comprehensive, interdisciplinary approach that would be at the crossroad of population-based prevention and individual-level clinical care, which is in line with a Global Health perspective. In the absence of a unifying theoretical framework to guide such interventions, a Dynamic Ecosystem Adaptation through the Allostasis (DEA-A) framework has been proposed, emphasizing the functional adaptation of individuals and organizations in symbiosis with their living ecosystem. While a conceptual model has been presented, this methodological contribution aims at illustrating the practical application of the DEA-A framework for planning Global Health interventions. The methodology combines Intervention Mapping and Cognitive and Behavioral Theory, extended to the ecosystem. Practical guidelines and supporting tools are provided to help public health providers and clinicians in establishing a functional ecosystem diagnosis of the issue; defining not only behavioral, but also emotional and cognitive change objectives (allostasis targets) expected for each stakeholder; and designing intervention plans targeting determinants of these allostasis. The discussion addresses implementation and evaluation perspectives of interventions based on the DEA-A framework, emphasizing the importance of considering change in its processual and ecosystem complexity. Lastly, encouragements for a deeper understanding of individual and ecosystem homeostasis/allostasis processes are made in order to promote more functional interventions.
Association between Weather and Self-Monitored Steps in Individuals with Prediabetes and Type 2 Diabetes in Sweden over Two Years
Authors: Woldamanuel Yohannes , Bergman Patrick , von Rosen Philip , Johansson Unn-Britt , Hagströmer Maria , Rossen Jenny
Published: March 2024

Background: Many studies have identified key factors affecting the rates of engagement in physical activity in older adults with chronic disease. Environmental conditions, such as weather variations, can present challenges for individuals with chronic diseases, such as type 2 diabetes when engaging in physical activity. However, few studies have investigated the influence of weather on daily steps in people with chronic diseases, especially those with prediabetes and type 2 diabetes. Objective: This study investigated the association between weather variations and daily self-monitored step counts over two years among individuals with prediabetes and type 2 diabetes in Sweden. Methods: The study is a secondary analysis using data from the Sophia Step Study, aimed at promoting physical activity among people with prediabetes and type 2 diabetes, which recruited participants from two urban primary care centers in Stockholm and one rural primary care center in southern Sweden over eight rounds. This study measured physical activity using step counters (Yamax Digiwalker SW200) and collected self-reported daily steps. Environmental factors such as daily average temperature, precipitation, and hours of sunshine were obtained from the Swedish Meteorological and Hydrological Institute. A robust linear mixed-effects model was applied as the analysis method. Results: There was no association found between weather variations and the number of steps taken on a daily basis. The analysis indicated that only 10% of the variation in daily steps could be explained by the average temperature, precipitation, and sunshine hours after controlling for age, gender, and BMI. Conversely, individual factors explained approximately 38% of the variation in the observations. Conclusion: This study revealed that there was no association between weather conditions and the number of daily steps reported by individuals with prediabetes and type 2 diabetes taking part in a physical activity intervention over two years. Despite the weather conditions, women and younger people reported more steps than their male and older counterparts.
Neighbourhood Walkability and Physical Activity during the COVID-19 Pandemic
Authors: Arifwidodo Sigit D. , Chandrasiri Orana
Published: March 2024

This study investigated whether living in a walkable neighbourhood could mitigate the adverse effects of the lockdown and closure of public open spaces during the COVID-19 pandemic on physical activity among adults in Bangkok, Thailand. We conducted a telephone survey with 579 respondents and collected information on their physical activity, access to green open spaces, neighbourhood walkability, and socioeconomic characteristics during the pandemic. Our study indicates that living in a walkable neighbourhood is associated with a higher likelihood of engaging in sufficient physical activity during the pandemic. Furthermore, we confirm the influence of socioeconomic factors and health behaviours on physical activity levels, aligning with previous research. Notably, our study highlights the significant association between access to green open spaces during lockdown and increased physical activity. These results underscore the importance of promoting walkable neighbourhoods and ensuring accessible green spaces to enhance physical activity and improve health outcomes during and beyond the pandemic.
Biodiesel Production by Methanolysis of Rapeseed Oil—Influence of SiO2/Al2O3 Ratio in BEA Zeolite Structure on Physicochemical and Catalytic Properties of Zeolite Systems with Alkaline Earth Oxides (MgO, CaO, SrO)
Authors: Szkudlarek Łukasz , Chałupka-Śpiewak Karolina , Maniukiewicz Waldemar , Nowosielska Magdalena , Szynkowska-Jóźwik Małgorzata Iwona , Mierczyński Paweł
Published: March 2024

Alkaline earth metal oxide (MgO, CaO, SrO) catalysts supported on BEA zeolite were prepared by a wet impregnation method and tested in the transesterification reaction of rapeseed oil with methanol towards the formation of biodiesel (FAMEs—fatty acid methyl esters). To assess the influence of the SiO2/Al2O3 ratio on the catalytic activity in the tested reaction, a BEA zeolite carrier material with different Si/Al ratios was used. The prepared catalysts were tested in the transesterification reaction at temperatures of 180 °C and 220 °C using a molar ratio of methanol/oil reagents of 9:1. The transesterification process was carried out for 2 h with the catalyst mass of 0.5 g. The oil conversion value and efficiency towards FAME formation were determined using the HPLC technique. The physicochemical properties of the catalysts were determined using the following research techniques: CO2-TPD, XRD, BET, FTIR, and SEM-EDS. The results of the catalytic activity showed that higher activity in the tested process was confirmed for the catalysts supported on the BEA zeolite characterized by the highest silica/alumina ratio for the reaction carried out at a temperature of 220 °C. The most active zeolite catalyst was the 10% CaO/BEA system (Si/Al = 300), which showed the highest triglyceride (TG) conversion of 90.5% and the second highest FAME yield of 94.6% in the transesterification reaction carried out at 220 °C. The high activity of this system is associated with its alkalinity, high value of the specific surface area, the size of the active phase crystallites, and its characteristic sorption properties in relation to methanol.
Detection of Sensitization Profiles with Cellular In Vitro Tests in Wheat Allergy Dependent on Augmentation Factors (WALDA)
Authors: Faihs Valentina , Schmalhofer Viktoria , Kugler Claudia , Bent Rebekka K. , Scherf Katharina A. , Lexhaller Barbara , Mortz Charlotte G. , Bindslev-Jensen Carsten , Biedermann Tilo , Skov Per S. , Eberlein Bernadette , Brockow Knut
Published: March 2024

Wheat allergy dependent on augmentation factors (WALDA) is the most common gluten allergy in adults. IgE-mediated sensitizations are directed towards ω5-gliadin but also to other wheat allergens. The value of the different in vitro cellular tests, namely the basophil activation test (BAT) and the active (aBHRA) and passive basophil histamine-release assays (pBHRA), in the detection of sensitization profiles beyond ω5-gliadin has not been compared. Therefore, 13 patients with challenge-confirmed, ω5-gliadin-positive WALDA and 11 healthy controls were enrolled. Specific IgE (sIgE), skin prick tests, BATs, aBHRA, and pBHRA were performed with allergen test solutions derived from wheat and other cereals, and results were analyzed and compared. This study reveals a distinct and highly individual reactivity of ω5-gliadin-positive WALDA patients to a range of wheat allergens beyond ω5-gliadin in cellular in vitro tests and SPT. In the BAT, for all tested allergens (gluten, high-molecular-weight glutenin subunits, α-amylase/trypsin inhibitors (ATIs), alcohol-free wheat beer, hydrolyzed wheat proteins (HWPs), rye gluten and secalins), basophil activation in patients was significantly higher than in controls (p = 0.004–p < 0.001). Similarly, significant histamine release was detected in the aBHRA for all test substances, exceeding the cut-off of 10 ng/mL in all tested allergens in 50% of patients. The dependency of tests on sIgE levels against ω5-gliadin differed; in the pBHRA, histamine release to any test substances could only be detected in patients with sIgE against ω5-gliadin ≥ 7.7 kU/L, whereas aBHRA also showed high reactivity in less sensitized patients. In most patients, reactivity to HWPs, ATIs, and rye allergens was observed. Additionally, alcohol-free wheat beer was first described as a promising test substance in ω5-gliadin-positive WALDA. Thus, BAT and aBHRA are valuable tools for the identification of sensitization profiles in WALDA.
Confinement of a Styryl Dye into Nanoporous Aluminophosphates: Channels vs. Cavities
Authors: Oliden-Sánchez Ainhoa , Sola-Llano Rebeca , Pérez-Pariente Joaquín , Gómez-Hortigüela Luis , Martínez-Martínez Virginia
Published: March 2024

Styryl dyes are generally poor fluorescent molecules inherited from their flexible molecular structures. However, their emissive properties can be boosted by restricting their molecular motions. A tight confinement into inorganic molecular sieves is a good strategy to yield highly fluorescent hybrid systems. In this work, we compare the confinement effect of two Mg-aluminophosphate zeotypes with distinct pore systems (the AEL framework, a one-dimensional channeled structure with elliptical pores of 6.5 Å × 4.0 Å, and the CHA framework, composed of large cavities of 6.7 Å × 10.0 Å connected by eight-ring narrower windows) for the encapsulation of 4-DASPI styryl dye (trans-4-[4-(Dimethylamino)styryl]-1-methylpyridinium iodide). The resultant hybrid systems display significantly improved photophysical features compared to 4-DASPI in solution as a result of tight confinement in both host inorganic frameworks. Molecular simulations reveal a tighter confinement of 4-DASPI in the elliptical channels of AEL, explaining its excellent photophysical properties. On the other hand, a singular arrangement of 4-DASPI dye is found when confined within the cavity-based CHA framework, where the 4-DASPI molecule spans along two adjacent cavities, with each aromatic ring sitting on these adjacent cavities and the polymethine chain residing within the narrower eight-ring window. However, despite the singularity of this host–guest arrangement, it provides less tight confinement for 4-DASPI than AEL, resulting in a slightly lower quantum yield.
Changes in Vitellogenin, Abdominal Lipid Content, and Hypopharyngeal Gland Development in Honey Bees Fed Diets with Different Protein Sources
Authors: Güneşdoğdu Mustafa , Sarıoğlu-Bozkurt Aybike , Şekeroğlu Ahmet , Abacı Samet Hasan
Published: March 2024

Honey bees play an important role in the pollination of flowering plants. When honey bee colonies are deficient in pollen, one of their main nutrients, protein supplements are required. In this study, the effects of diets with six different protein sources on the physiological characteristics of worker bees (vitellogenin (Vg), abdominal lipid content (ALC), hypopharyngeal gland (HPG)) and consumption were investigated. The protein sources of the diets (diet I, …, diet VI) included pollen, spirulina dust (Arthrospira platensis Gomont), fresh egg yolk, lyophilized lactose-free skimmed milk powder, active fresh yeast, and ApiProtein. It was identified that consumption by worker bees was highest in the diet group supplemented with spirulina (diet II). Although there was no statistical difference regarding the Vg content in the hemolymph, numerically, the highest content was found in diet group IV (lyophilized lactose-free skimmed milk powder) (4.73 ± 0.03 ng/mL). ALC and HPG were highest in the group fed diet II. These results suggest that offering honey bees diets with certain protein sources can support their physiological traits.
Evaluating Community-Based Intrathecal Baclofen Therapy: Effectiveness, Safety, and Feasibility
Authors: van der Gaag Simone M. E. , Frankema Sander P. G. , van der Ploeg Eva S. , Baart Sara J. , Huygen Frank J. M. P.
Published: March 2024

Background: Intrathecal baclofen (ITB) is used for the treatment of intractable spasticity. The burden of traveling for ITB screening and aftercare is problematic for nursing home residents with severe spasticity and seems to result in undertreatment of spasticity. The aim of this study is to evaluate the effectiveness, safety, and feasibility of ITB for nursing home residents treated in their home, describing the selection phase, the initial trial of ITB, and aftercare up to 3 months after implantation of an ITB pump. Methods: This retrospective database study included immobile, adult nursing home residents with severe spasticity, referred to an Ambulatory Care Clinic between 2016 and 2021. When eligible, an ITB trial was performed by ITB experts in the nursing home. If a permanent pump was implanted, dose titration and aftercare were performed on location. Results: A total of 102 patients were referred; 80 underwent an ITB trial on location, and 94% improved significantly on the Modified Ashworth Scale and clonus scale pre-ITB trial versus post-ITB trial, as well as at 3 months post-implantation. There was a low incidence of adverse events, mostly procedure- and drug-related. Conclusions: This study indicates that selection, testing, and aftercare for ITB on location is effective and safe.
Anti-Inflammatory, Antipyretic, and Analgesic Potential of Chitin and Chitosan Derived from Cockroaches (Periplaneta americana) and Termites
Authors: Asad Khushbakht , Shams Sumaira , Ibáñez-Arancibia Eliana , De los Ríos-Escalante Patricio R. , Badshah Farhad , Ahmad Farooq , Khan Muhammad Salman , Khan Asar
Published: March 2024

The chitin and chitosan biopolymers are extremely valuable because of their numerous industrial and pharmacological uses. Chitin and chitosan were extracted from the exoskeleton of Periplaneta americana (cockroaches) and termites using various acid and alkali techniques. The extraction process involves an initial demineralization step, during which integument dry powder was subjected to 500 mL (2.07 mol/L) of concentrated HCl at 100 degrees Celsius for 30 min, followed by meticulous rinsing with distilled water to restore the pH to its baseline. Deproteinization was conducted at 80 degrees Celsius using 500 mL (1 mol/L) of NaOH solution, which was repeated for 24 h. A total of 250 mL (0.06 mol/L) of NaOH was added at 100 degrees Celsius for 4 h to obtain chitosan, followed by extensive washing and subsequent drying. FTIR analysis was used to identify the functional groups in Periplaneta americana and termites. The crystallinity of these biopolymers, which have a face-centered cubic structure, was determined by X-ray diffraction analysis. This study assessed the analgesic properties of chitin and chitosan via an acetic-acid-induced writhing test in mice, revealing a significant reduction in writhing behavior following the chitin and chitosan extract. Notably, chitin exhibits the highest degree of analgesic activity compared to chitosan. Both chitin and chitosan show anti-inflammatory effects, with chitosan absorbing proton ions at sites of inflammation, while chitin effectively inhibits ear edema and elicits an analgesic response in mice. Furthermore, the present study revealed antipyretic activity, with termite chitin demonstrating the most significant effect at a concentration of 500 µL/mL, followed by chitosan and chitin at 100 µL/mL. These findings indicate the potential of using chitin and chitosan derived from termites and Periplaneta americana as natural anti-inflammatory compounds, implying prospective uses in anti-inflammatory, antipyretic, and analgesic capabilities.
Clinical Outcomes of MOK Pharmacopuncture in an Elderly Male Patient with Hypothyroidism—A Case Report and Literature Review
Authors: Jeong Jin-Ho , Hwang Ji Hye
Published: March 2024

Hypothyroidism is more common in women and individuals between 30 and 50 years old. This case report depicts the clinical outcomes of MOK pharmacopuncture, a type of Korean medicine treatment, for an elderly male patient with hypothyroidism who was on long-term L-thyroxine (LT4) therapy but still felt chronically lethargic and tired and was generally in poor health. A 72-year-old Korean man has been on LT4 since being diagnosed with hypothyroidism 16 years ago and has tried to discontinue hormone supplements in the past. The patient was treated with MOK pharmacopuncture, mainly at the ST10 acupoint, twice a week for four months. Following the treatment, the T3, free-T4, and TPO Ab levels and thyroiditis status on ultrasound showed improvement. Additionally, there were a normalization of ESR levels, an enhancement in the quality of life, a reduction in depression scores, an improvement in the antioxidant status, and an alleviation of major symptoms when compared to pre-treatment conditions. This case report demonstrates the potential of MOK pharmacopuncture as a complementary treatment for an elderly man with hypothyroidism who had a poor quality of life due to fatigue and lethargy despite LT4 treatment.
Pathology Laboratory Archives: Conservation Quality of Nucleic Acids and Proteins for NSCLC Molecular Testing
Authors: Eccher Albino , Seminati Davide , L’Imperio Vincenzo , Casati Gabriele , Pilla Daniela , Malapelle Umberto , Piga Isabella , Bindi Greta , Marando Alessandro , Bonoldi Emanuela , Dainese Emanuele , Riefolo Mattia , D’Errico Antonia , Costantini Matteo , Lugli Alberto , Grassi Stefano , Scarpa Aldo , Dei Tos Angelo Paolo , Pagni Fabio
Published: March 2024

In the molecular era, proper archival conditions within pathology laboratories are crucial, especially for formalin-fixed paraffin-embedded (FFPE) tissue specimens retrieved years after the original diagnosis. Indeed, improper preservation can impact the integrity of nucleic acids and protein antigens. This study evaluates the quality status of stored FFPE blocks using multilevel omics approaches. FFPE blocks from 45 Non-Small Cell Lung Carcinoma (NSCLC) cases were analyzed. The blocks were collected from six different pathology archives across Italy with distinct environmental characteristics. Nucleic acids’ quantity and quality, as well as protein antigens, were assessed using various techniques, including MALDI-MSI. RNA was quantitatively higher, but more fragmented, compared to DNA. DNA quantity and quality were suitable for molecular analyses in 94.4% and 62.3% of samples, respectively. RNA quantity was adequate across all samples, but it was optimal only in 22.3% of cases. DNA quality started to deteriorate after 6–8 years, whereas RNA quality diminished only after 10 years of storage. These data might suggest a particular DNA susceptibility to FFPE blocks conservation. Immunohistochemical intensity decreased significantly after 6–8 years of storage, and MALDI-MSI analysis revealed that younger tissue blocks contained more unique proteomic signals than the older ones. This study emphasizes the importance of proper FFPE archiving conditions for molecular analyses. Governance should prioritize attention to pathology archives to ensure quality preservation and optimize predictive testing. By elucidating the nuances of FFPE block storage, this research paves the way for enhanced molecular diagnostics and therapeutic insights regarding oncology and beyond.
Evaluation of High-Frequency Measurement Errors from Turned Surface Topography Data Using Machine Learning Methods
Authors: Podulka Przemysław , Kulisz Monika , Antosz Katarzyna
Published: March 2024

Manufacturing processes in industry applications are often controlled by the evaluation of surface topography. Topography, in its overall performance, includes form, waviness, and roughness. Methods of measurement of surface roughness can be roughly divided into tactile and contactless techniques. The latter ones are much faster but sensitive to external disturbances from the environment. One type of external source error, while the measurement of surface topography occurs, is a high-frequency noise. This noise originates from the vibration of the measuring system. In this study, the methods for reducing high-frequency errors from the results of contactless roughness measurements of turned surfaces were supported by machine learning methods. This research delves into optimizing filtration methods for surface topography measurements through the application of machine learning models, focusing on enhancing the accuracy of surface roughness assessments. By examining turned surfaces under specific machining conditions and employing a variety of digital filters, the study identifies the Gaussian regression filter and spline filter as the most effective methods at a 22.5 µm cut-off. Utilizing neural networks, support vector machines, and decision trees, the research demonstrates the superior performance of SVMs, achieving remarkable accuracy and sensitivity in predicting optimal filtration methods.