bluuebunny/crossref_metadata_2025 huggingface dataset:
The Modern Nile Delta Continental Shelf, with an Evolving Record of Relict Deposits Displaced and Altered by Sediment Dynamics
Authors: Frihy Omran E. , Stanley Jean-Daniel
Published: June 2023

The most extensive coverage of surficial sediment samples collected to date on Egypt’s Nile Delta coast and shelf is needed to better define sediment dispersal patterns across this setting’s rapidly eroding margin. Changes in time are now induced by River Nile sediment cutoff by dams, sea level rise, marked shelf subsidence, and regional climate changes, which have altered the amounts and components of sediments; these require replacement, along with the implementation of more effective coastal protection measures. Multiple computer-generated offshore maps depict the distributions and proportions of sand, silt, and mud; the mean grain size and standard deviation (sorting); heavy mineral concentrations; and carbonate content. Heavy mineral lobes at the coast and offshore identify former Nile branch sites. Channel lobes extending seaward resulted from their progradational phase and from the delta’s altered sedimentation from the early to late Holocene. The progressive deposition and erosion of these fossil fluvial lobes, and of two active Nile channels, selectively removed their quartz and less dense minerals, thus concentrating heavy minerals on the coast and inner shelf. The prolonged dispersal of original sediment effluence from relict and recent Nile tributaries induced variable depositional patterns on the present shelf. These coastal depocenters, along with extensive sand, silt, and mud from shelf sediments, were reworked further seaward and dispersed by bottom currents, thus masking most previous onshore-to-offshore transport patterns. The major surficial features document long-term responses to the diverse dispersal that influenced the shoreline to the outer shelf deposits from the Pleistocene to the present.
Age-Friendly Ecosystems: Expert Voices from the Field
Authors: Fulmer Terry , Dash Kim , Shue Jody , Chang JiHo , Huang Jessica , Maglich Abby
Published: June 2023

(1) Background: With the growth of the age-friendly movement, age-friendly ecosystems (AFE) garnered more attention. The successful development of an AFE is contingent on unified efforts across different stakeholders; however, limited efforts were made to help create a common understanding of the necessary components of an AFE. (2) Methodology: In response, The John A. Hartford Foundation and The Age-Friendly Institute hosted a series of convenings of international experts to identify a working definition of the characteristics composing an AFE. The goal of these convenings was to provide a foundation on which to unite cross-sector age-friendly work. (3) Results: This paper discussed the findings of the convenings and provided a framework from which future age-friendly work must draw upon. (4) Conclusions: This paper presented a necessary change in how we conceive AFEs.
Tragedy, Tragic Irony, and War: A Dialectical Approach
Authors: Airaksinen Timo
Published: June 2023

Tragic irony may mean the dramatic irony in scripted tragedy (tragic play). The audience can predict the regrettable outcome on the stage before the main characters do. I focus on non-scripted events and their tragic aspects. Colloquially, disaster and tragedy are synonyms, but this is misleading. Tragedy means a disaster in special circumstances, which I suggest we can read ironically. This is to say, as I argue, tragedy is necessarily ironic. I read Richard Rorty on irony and Hegel on tragic irony and cunning of reason. My aim is to redescribe real-life conflicts by using the dialectical understanding of irony and tragedy. Following Rorty and Hegel, I apply their theories of identity to real tragedies. The validation of the theory of literary criticism is a practical matter. My key illustrations come from modern wars; wars are and cause disasters, and thus I expect we can discover cases of tragic irony in factual and counterfactual contexts. Sometimes, the losses and suffering would have been meaningless regardless of the war’s outcome. The winner suffers, but it would have been better not to win. The losers suffer, but it would not have been better had they won. A total defeat would have been better than a conditional one. These redescriptions show the ironic differences between disaster and tragedy in non-scripted contexts—and all these cases are controversial.
Assessing the Feasibility and Acceptability of a Primary Care Socio-Ecological Approach to Improve Physical Activity Adherence among People with Type 2 Diabetes: The SENWI Project
Authors: Jabardo-Camprubí Guillem , Puig-Ribera Anna , Donat-Roca Rafel , Farrés-Godayol Pau , Nazar-Gonzalez Sebastian , Sitjà-Rabert Mercè , Espelt Albert , Bort-Roig Judit
Published: June 2023

Maintaining an active lifestyle is a key health behavior in people with type 2 diabetes (T2D). This study assessed the feasibility and acceptability of a socio-ecological Nordic walking intervention (SENWI) to enhance healthy behaviors in primary healthcare settings. Participants included individuals with T2D (n = 33; age 70 (95% CI 69–74)) and healthcare professionals (HCPs, n = 3). T2D participants were randomly assigned to a SENWI, active comparator, or control group for twelve weeks. Feasibility and acceptability were evaluated based on a mixed methodology. Quantitative data reported adherence information, differences between follow-up and dropout participants and pre- and post-intervention on physical activity, sedentary behavior, and health outcomes. Qualitative data acquisition was performed using focus groups and semi-structured interviews and analyzed using thematic analysis. Thirty-three T2D invited participants were recruited, and twenty-two (66.7%) provided post-intervention data. The SENWI was deemed acceptable and feasible, but participants highlighted the need to improve options, group schedules, gender inequities, and the intervention’s expiration date. Healthcare professionals expressed a lack of institutional support and resources. Nevertheless, no significant difference between the SENWI follow-up and dropout participants or pre- and post- intervention was found (only between the active comparator and control group in the physical quality of life domain). Implementing the SENWI in primary healthcare settings is feasible and acceptable in real-world conditions. However, a larger sample is needed to assess the program’s effectiveness in improving healthy behaviors and its impact on health-related outcomes in the long term.
A Tale of Two Audiences: Formative Research and Campaign Development for Two Different Latino Audiences, to Improve COVID-19 Prevention Behavior
Authors: Bonilla Altera Dianna , Cabassa Imani , Martinez-Garcia Genevieve
Published: June 2023

The COVID-19 pandemic disproportionately affected the Latino population in the United States, further exacerbating the existing racial and ethnic health disparities that this group faces. While government health entities rushed to develop COVID-19 prevention educational materials in Spanish, these failed to recognize the unique motivators and barriers that move different Latino audience segments to act. We conducted five online focus groups with two different Latino audience segments, general Latino people, and Latino migrant workers, to assess their experience navigating the pandemic, their engagement in preventive behavior, and their consumption of health news. While the general Latino audience had higher levels of social capital and established preventive healthcare, they were more skeptical about getting the COVID-19 vaccine. Migrant workers needed to be vaccinated to retain their jobs, and saw the vaccine as the only way to keep their families healthy. We used the focus group results to develop two different creative concepts that aligned with each audience’s unique experience. Our study highlights the importance of developing hyper-focused messages, responsive to the experience of distinct audience segments, for maximum impact.
Revitalizing General Practice: The Critical Role of Medical Schools in Addressing the Primary Care Physician Shortage
Authors: Wiedermann Christian J.
Published: June 2023

The shortage of primary care physicians is a growing crisis that threatens the stability and effectiveness of healthcare systems. This paper explores a multi-pronged approach to addressing this issue by focusing on the modernization of medical curricula, the establishment of new medical schools, fostering collaboration between institutions, and implementing policy innovations. The cases of South Tyrol, Italy, and Tyrol, Austria, are examined to highlight the challenges faced in establishing new medical schools. This paper proposes that a comprehensive strategy, including the incorporation of general practice content and experience in medical education, is crucial for preparing future physicians for careers in primary care. Furthermore, intensifying efforts to establish new medical schools, particularly in regions such as South Tyrol, which lack native-language medical university education, can provide additional benefits in addressing regional needs and augmenting the number of graduates. Collaboration between existing and new medical schools, regional partnerships, and policy innovations are essential to support the establishment of institutions with a particular focus on general practice and the modernization of curricula at existing universities. By embracing this approach, stakeholders can collectively shape the medical education landscape and address the growing crisis of physician shortages in primary care.
Peristomal Skin Complications: Detailed Analysis of a Web-Based Survey and Predictive Risk Factors
Authors: Guerra Eliana , Denti Francesco Carlo , Di Pasquale Cristina , Caroppo Francesca , Angileri Luisa , Cioni Margherita , Parodi Aurora , Fortina Anna Belloni , Ferrucci Silvia , Burlando Martina
Published: June 2023

Patients with a stoma are at risk of developing peristomal skin complications (PSCs) that can negatively impact their quality of life. This study aims to identify potential risk factors for dermatitis, pruritis/xerosis, infections, and ulcerations among patients with a stoma and evaluate preventive measures. This cross-sectional study involved data regarding 232 Italian patients with a stoma. A questionnaire was used to collect patient characteristics, comorbidities, and stoma management data. The most frequent PSCs observed were dermatitis and pruritis/xerosis in approximately 60% of patients. Psoriasis was strongly correlated with dermatitis, while being overweight or obese increased the risk of pruritis/xerosis. Class 2 obesity and atopic dermatitis were associated with an increased risk of infections. Being underweight, completely nonautonomous, and having inflammatory bowel disease were associated with a higher risk of ulcerations, while radiotherapy was a strong risk factor for ulceration. Preventive measures such as using hydrocolloid barriers, TNT gauze cleansing, and low pH detergent were effective in preventing dermatitis. Appropriate stoma care and maintenance, including the use of protective film and careful monitoring of weight and comorbidities, are crucial in minimizing the risk of complications associated with a stoma.
Osteological Evidence of Possible Tuberculosis from the Early Medieval Age (6th–11th Century), Northern Italy
Authors: Larentis Omar , Pangrazzi Caterina , Tonina Enrica
Published: June 2023

We discuss the probable presence of tuberculosis in non-adults of a medieval rural community in northern Italy with a biocultural perspective. Before birth, mother and child have a closely interconnected relationship, as suggested by the role of microchimerism on maternal health. To better understand maternal–child health and related stress factors, paleopathology has investigated the potential of this relationship in recent years. Diseases with environmental development factors such as infections depend on and are strongly affected by maternal control variables such as, for example, breastfeeding and the growth environment. This article presents ISZ2, the second possible case of childhood tuberculosis identified in northeastern Italy through recent paleopathological criteria. The subject is of primary importance as it not only represents a direct testimony of this infectious disease otherwise known only from historical sources, but also increases our knowledge on the state of health of infants in this area, which to date are scarcely analyzed.
Coatings Based on Gelatin and Chitosan in the Conservation of Papaya (Carica papaya L.) Minimally Processed
Authors: Silva Kátia Gomes da , Cavalcanti Mônica Tejo , Martinsa Laesio Pereira , Alves Rita de Cássia , Lucena Fernando Azevedo de , Santos Max Suel Alves , Silva Samarone Xavier da , Costa Franciscleudo Bezerra da , Moreira Inacia dos Santos , Pereira Emmanuel Moreira
Published: June 2023

Minimally processed fruits undergo changes that require careful monitoring. This study aimed to assess the effectiveness of edible coatings based on chitosan and gelatin using various application techniques for minimally processed papayas. The treatments included: control (distilled water), 1% chitosan, 4% gelatin, 1% chitosan + 4% gelatin (blend), and 1% chitosan with a 4% gelatin undercoat (layer-by-layer). The coatings’ infrared spectroscopies (FTIR) were analyzed, and the fruits’ fresh mass, firmness, pulp color (L*, a*, b*, and hue angle), pH, titratable acidity, soluble solids, ascorbic acid, phenolic compounds, lycopene, β-carotene, total sugars, and catalase activity were measured. The results revealed that gelatin and the layer-by-layer treatment positively influenced the preservation of minimally processed papaya. These coatings effectively reduced fresh mass loss while maintaining firmness and the characteristic orange color of the mesocarp. Furthermore, the treated samples consistently exhibited low soluble solids content during the storage period, with minimal variations in acidity, thereby influencing the maturation process. Gelatin coatings demonstrated low polyphenol content, while the layer-by-layer treatment showed no significant changes in vitamin C levels. Lycopene and β-carotene levels remained stable throughout the storage period, with a slight increase observed in total sugars. Consequently, the application of gelatin polymers and the undercoat treatment (LBL) represents a viable alternative for extending the shelf life of minimally processed papayas for at least eight days.
Micromorphology of Barleria albostellata (Grey Barleria) Flower and Pollen Grains
Authors: Gangaram Serisha , Naidoo Yougasphree , Dewir Yaser Hassan , Singh Moganavelli , Magyar-Tábori Katalin
Published: June 2023

Barleria albostellata C.B. Clarke (grey barleria, Acanthaceae) is an indigenous shrub to South Africa and has been relatively understudied. This shrub is a valuable medicinal plant with a wide spectrum of antibacterial and anti-inflammatory activities. Detailed studies on the floral and pollen morphology on B. albostellata are rare. This study was conducted to observe the morphology of the flower and pollen grains using stereomicroscopy and scanning electron microscopy (SEM). Morphological observations showed numerous non-glandular trichomes on the bracteoles and bracts of B. albostellata. Three types of trichomes were identified on these structures: I—unicellular, II—multangulate-dendritic branched non-glandular trichomes, and III—capitate glandular trichomes. A taxonomical description of the floral structures using stereo and SEM micrographs is provided. SEM micrographs revealed the pollen grains as globose tricolporate with a rough honeycomb exine, and small granules inside the lumina. The diameter of the pollen grains was 77.53 ± 5.63 μm, whereas the aperture of these grains was 14.31 ± 0.59 µm. This study provides insight into the floral biology of B. albostellata, and the results presented here will add to the body of knowledge and encourage further research on this species.
Table 2 Fallacy in Descriptive Epidemiology: Bringing Machine Learning to the Table
Authors: Dharma Christoffer , Fu Rui , Chaiton Michael
Published: June 2023

There is a lack of rigorous methodological development for descriptive epidemiology, where the goal is to describe and identify the most important associations with an outcome given a large set of potential predictors. This has often led to the Table 2 fallacy, where one presents the coefficient estimates for all covariates from a single multivariable regression model, which are often uninterpretable in a descriptive analysis. We argue that machine learning (ML) is a potential solution to this problem. We illustrate the power of ML with an example analysis identifying the most important predictors of alcohol abuse among sexual minority youth. The framework we propose for this analysis is as follows: (1) Identify a few ML methods for the analysis, (2) optimize the parameters using the whole data with a nested cross-validation approach, (3) rank the variables using variable importance scores, (4) present partial dependence plots (PDP) to illustrate the association between the important variables and the outcome, (5) and identify the strength of the interaction terms using the PDPs. We discuss the potential strengths and weaknesses of using ML methods for descriptive analysis and future directions for research. R codes to reproduce these analyses are provided, which we invite other researchers to use.
Complex Sex Differences in Life Expectancy in French Guiana
Authors: Nacher Mathieu , Basurko Célia , Imounga Laure Manuella , Wang Qiannan , Van Melle Astrid , Lucarelli Aude , Adenis Antoine , Alsibai Kinan Drak , Hcini Najeh , Sabbah Nadia
Published: June 2023

In the complex context of French Guiana, different vulnerabilities and different risk factors between genders may lead to complex differences in health outcomes, mortality, and life expectancy. Our aim was, thus, to compare male and female mortality and life expectancy, to compare it between French Guiana and mainland France, and to look at temporal trends and the main specific causes of death in order to identify actionable singularities. National databases were used to obtain life expectancy at birth, at 20, 40, and 60 years, and mortality statistics. Standardized death rates and causes of death for French Guiana and mainland France were obtained through the CEPIDC, which analyzes information from death certificates. When comparing with mainland France, life expectancy at birth was significantly shorter both in males and females (mean = −2.9 years); life expectancy at 20 years, which allows to remove the effect of the greater child mortality in French Guiana, was also shorter in French Guiana for males (mean = −1.8 years) and females (mean = −2 years). The differences between mainland France and French Guiana regarding life expectancy at 40 and 60 years (mean = −1.5 and −1.3 years) was mainly found among females, males in French Guiana life expectancy at 40 and 60 years was closer to that in mainland France (mean = −0.8 and −0.6 years). Although they have a greater life expectancy at birth than men, women in French Guiana are substantially more affected by overweight/obesity and type 2 diabetes. The observed patterns of life expectancy at different ages presumably reflect the burden of external causes and AIDS in males and perhaps metabolic diseases in women.
Domestic Violence against LGBTI People: Perspectives of Portuguese Education Professionals
Authors: Sousa Edgar , Neves Sofia , Ferreira Mafalda , Topa Joana , Vieira Cristina Pereira , Borges Janete , Costa Rodrigo , Lira André
Published: June 2023

Lesbian, gay, bisexual, trans and intersex (LGBTI) people are more likely to be exposed to domestic violence than the rest of the population. Using a descriptive qualitative methodology, 28 professionals from the educational sector, aged between 28 and 64 years old (M = 44.5), were interviewed to describe and understand their perspectives on the sector’s ability to support, intervene and get involved with LGBTI people and, particularly, with victims or former victims of domestic violence. Through a thematic content analysis, three main themes emerged: (i) life trajectories of LGBTI people; (ii) domestic violence perpetrated against LGBTI people; and (iii) training of the educational sector to intervene with LGBTI people. The results show that Portuguese education professionals are not trained to recognize and intervene with LGBTI people and, in particular, with those who are victims of domestic violence, since they are unaware of the existence of protocols and/or guidelines for detecting and preventing risk situations among students. Furthermore, the curricular plan fails in the coverage of domestic violence and LGBTI-related topics, although the National Strategy for Citizenship Education has been implemented in Portugal since 2017. Findings suggest the need to invest in education professionals’ training.
Companion Animal Fostering as Health Promotion: A Literature Review
Authors: Roseveare Christine , Breheny Mary , Mansvelt Juliana , Murray Linda , Wilkie Marg , Gates M. Carolyn
Published: June 2023

There is growing interest in the health-promoting potential of human-companion animal relationships from a broad public health perspective while acknowledging barriers to ownership, particularly for older adults. Companion animal fostering is an alternative to pet ownership that aligns with the Ottawa Charter health promotion principle that caring for others in everyday settings promotes health. This narrative review of the literature on companion animal fostering draws on Te Whare Tapa Whā (the four-sided house), an indigenous model of health that is influential in Aotearoa/New Zealand, and the Ottawa Charter. We found that companion animal fostering can be considered health-promoting for human and non-human animals, using a broad and multidimensional understanding of health. As well as improving the long-term outcomes for homeless animals, companion animal fostering has the potential to promote the health of the individuals, families, and communities who provide foster homes. Our review highlights the importance of health promoters considering the reciprocal relationship between human and animal health. Future research should explore different aspects of human and non-human health, perspectives of different types of fosterers in different settings and communities, barriers to fostering, and methods that explore the role of caring for a wider range of companion animals in creating and sustaining wellbeing.
Disclosure of Key Audit Matters: European Listed Companies’ Evidence on Related Parties Transactions
Authors: Pasc Lioara-Veronica , Hategan Camelia-Daniela
Published: June 2023

The growing expenses, dependence on IT for business operations, and growing requirements regarding related party transaction (RPT) reporting impose the need for increased attention to this area. The paper’s objective is to examine the nature of RPTs, identified by auditors as a key audit matter (KAMs), challenges and solutions to problems related to risk management, and the detection of factors affecting audit quality. The research methodology is qualitative, with an analysis of the level of disclosure of KAMs reported by auditors from the Related Parties category, grouped by type of auditors, their opinion, year, country, and fields of activity. Data were collected from the Audit Analytics database and filtered by category KAM: Related parties, period 2013–2021. The selection resulted in 111 companies reporting 248 KAMs related to RPTs, from which most were reported in 2017–2019. Of these, nearly two-thirds were reported by auditors from the Big4 category. Most KAMs were reported by companies in the U.K., Germany, and France, and the industries with the most KAMs were finance, insurance, and real estate. In conclusion, there are factors that can affect audit quality due to the reporting of RPTs, but by identifying them, the audit process can be better managed, thus increasing its efficiency.
End-to-Side vs. Free Graft Nerve Reconstruction—Experimental Study on Rats
Authors: Czarnecki Piotr , Huber Juliusz , Szymankiewicz-Szukała Agnieszka , Górecki Michał , Romanowski Leszek
Published: June 2023

The long history of regeneration nerve research indicates many clinical problems with surgical reconstruction to be resolved. One of the promising surgical techniques in specific clinical conditions is end-to-side neurorrhaphy (ETS), described and then repeated with different efficiency in the 1990s of the twentieth century. There are no reliable data on the quality of recipient nerve regeneration, possible donor nerve damage, and epineural window technique necessary to be performed. This research attempts to evaluate the possible regeneration after end-to-side neurorrhaphy, its quality, potential donor nerve damage, and the influence of epineural windows on regeneration efficiency. Forty-five female Wistar rats were divided into three equal groups, and various surgical technics were applied: A—ETS without epineural window, B—ETS with epineural window, and C—free graft reconstruction. The right peroneal nerve was operated on, and the tibial nerve was selected as a donor. After 24 weeks, the regeneration was evaluated by (1) footprint analysis every two weeks with PFI (peroneal nerve function index), TFI (tibial nerve function index), and SFI (sciatic nerve function index) calculations; (2) the amplitude and latency measurements of motor evoked potentials parameters recorded on both sides of the peroneal and tibial nerves when electroneurography with direct sciatic nerve electrical stimulation and indirect magnetic stimulation were applied; (3) histomorphometry with digital conversion of a transverse semithin nerve section, with axon count, fibers diameter, and calculation of axon area with a semiautomated method were performed. There was no statistically significant difference between the groups investigated in all the parameters. The functional indexes stabilized after eight weeks (PFI) and six weeks (TFI and SFI) and were positively time related. The lower amplitude of tibial nerve potential in groups A and B was proven compared to the non-operated side. Neurophysiological parameters of the peroneal nerve did not differ significantly. Histomorphometry revealed significantly lower diameter and area of axons in operated peroneal nerves compared to non-operated nerves. The axon count was at a normal level in every group. Tibial nerve parameters did not differ from non-operated values. Regeneration of the peroneal nerve after ETS was ascertained to be at the same level as in the case of free graft reconstruction. Peroneal nerves after ETS and free graft reconstruction were ascertained to have a lower diameter and area than non-operated ones. The technique of an epineural window does not influence the regeneration result of the peroneal nerve. The tibial nerve motor evoked potentials were characterized by lower amplitudes in ETS groups, which could indicate axonal impairment.
Reduced Levels of Selenium and Thioredoxin Reductase in the Thoracic Aorta Could Contribute to Aneurysm Formation in Patients with Marfan Syndrome
Authors: Soto María Elena , Pérez-Torres Israel , Manzano-Pech Linaloe , Soria-Castro Elizabeth , Morales-Marín Almilcar , Ramírez-Marroquín Edgar Samuel , Martínez-Hernández Humberto , Herrera-Alarcón Valentín , Guarner-Lans Verónica
Published: June 2023

Marfan syndrome (MFS) is an autosomal dominant disorder caused by a heterozygous mutation of the FBN1 gene. MFS patients present oxidative stress that disturbs redox homeostasis. Redox homeostasis depends in part on the enzymatic antioxidant system, which includes thioredoxin reductase (TrxR) and glutathione peroxidases (GPx), both of which require an adequate concentration of selenium (Se). Therefore, the aim of this study was to determine if Se levels are decreased in the TAA of patients with MFS since this could contribute to the formation of an aneurysm in these patients. The results show that interleukins IL-1β, IL-6 TGF-β1, and TNF-α (p ≤ 0.03), and carbonylation (p ≤ 0.03) were increased in the TAA of patients with MFS in comparison with control subjects, while Se, thiols (p = 0.02), TrxR, and GPx (p ≤ 0.001) were decreased. TLR4 and NOX1 (p ≤ 0.03), MMP9 and MMP2 (p = 0.04) and NOS2 (p < 0.001) were also increased. Therefore, Se concentrations are decreased in the TAA of MFS, which can contribute to a decrease in the activities of TrxR and GPx, and thiol groups. A decrease in the activities of these enzymes can lead to the loss of redox homeostasis, which can, in turn, lead to an increase in the pro-inflammatory interleukins associated with the overexpression of MMP9 and MMP2.
C-X-C Motif Chemokine 3 Promotes the Inflammatory Response of Microglia after Escherichia coli-Induced Meningitis
Authors: Qu Xinyi , Dou Beibei , Yang Ruicheng , Tan Chen , Chen Huanchun , Wang Xiangru
Published: June 2023

Meningitis is a major clinical manifestation of Escherichia coli (E. coli) infection characterized by inflammation of the meninges and subarachnoid space. Many chemokines are secreted during meningitic E. coli infection, of which C-X-C motif chemokine 3 (CXCL3) is the most highly expressed. However, it is unclear how CXCL3 plays a role in meningitic E. coli infection. Therefore, this study used in vitro and in vivo assays to clarify these contributions and to identify novel therapeutic targets for central nervous system inflammation. We found a significantly upregulated expression of CXCL3 in human brain microvascular endothelial cells and U251 cells after meningitic E. coli infection, and the CXCL3 receptor, C-X-C motif chemokine receptor 2 (CXCR2), was expressed in microglia. Furthermore, CXCL3 induced M1 microglia by selectively activating mitogen-activated protein kinases signaling and significantly upregulating tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, nitric oxide synthase 2 (NOS2), and cluster of differentiation 86 (CD86) expression levels, promoting an inflammatory response. Our findings clarify the role of CXCL3 in meningitic E. coli-induced neuroinflammation and demonstrate that CXCL3 may be a potential therapeutic target for future investigation and prevention of E. coli-induced neuroinflammation.
A Diet Enriched with Lacticaseibacillus rhamnosus HN001 and Milk Fat Globule Membrane Alters the Gut Microbiota and Decreases Amygdala GABA a Receptor Expression in Stress-Sensitive Rats
Authors: Dalziel Julie E. , Zobel Gosia , Dewhurst Hilary , Hurst Charlotte , Olson Trent , Rodriguez-Sanchez Raquel , Mace Louise , Parkar Nabil , Thum Caroline , Hannaford Rina , Fraser Karl , MacGibbon Alastair , Bassett Shalome A. , Dekker James , Anderson Rachel C. , Young Wayne
Published: June 2023

Brain signalling pathways involved in subclinical anxiety and depressed mood can be modulated via the gut brain axis (GBA), providing the potential for diet and dietary components to affect mood. We investigated behavioural, physiological and gut microbiome responses to the Lacticaseibacillus rhamnosus strain HN001 (LactoB HN001™), which has been shown to reduce postpartum anxiety and depression, and a milk fat globule membrane-enriched product, Lipid 70 (SurestartTM MFGM Lipid 70), which has been implicated in memory in stress-susceptible Wistar Kyoto rats. We examined behaviour in the open field, elevated plus maze and novel object recognition tests in conjunction with the expression of host genes in neuro-signalling pathways, and we also assessed brain lipidomics. Treatment-induced alterations in the caecal microbiome and short-chain fatty acid (SCFA) profiles were also assessed. Neither ingredient induced behavioural changes or altered the brain lipidome (separately or when combined). However, with regard to brain gene expression, the L. rhamnosus HN001 + Lipid 70 combination produced a synergistic effect, reducing GABAA subunit expression in the amygdala (Gabre, Gat3, Gabrg1) and hippocampus (Gabrd). Treatment with L. rhamnosus HN001 alone altered expression of the metabotropic glutamate receptor (Grm4) in the amygdala but produced only minor changes in gut microbiota composition. In contrast, Lipid 70 alone did not alter brain gene expression but produced a significant shift in the gut microbiota profile. Under the conditions used, there was no observed effect on rat behaviour for the ingredient combination. However, the enhancement of brain gene expression by L. rhamnosus HN001 + Lipid 70 implicates synergistic actions on region-specific neural pathways associated with fear, anxiety, depression and memory. A significant shift in the gut microbiota profile also occurred that was mainly attributable to Lipid 70.
Adipose Tissue-Derived Mesenchymal Stem Cell-Derived Exosomes Promote Wound Healing and Tissue Regeneration
Authors: Lee Jun Ho , Won Yu Jin , Kim Hail , Choi Minji , Lee Esther , Ryoou Bumsik , Lee Seok-Geun , Cho Byong Seung
Published: June 2023

Wound healing is a complex process involving cell proliferation, migration, and extracellular matrix (ECM) remodeling. Extracellular vesicles (EVs) or exosomes derived from adipose tissue-derived stem cells (ASCs) are emerging as promising alternatives to cell therapy for advanced wound healing. Hyaluronic acid (HA), a major component of the skin ECM, is widely utilized in wound dressings and dermal fillers. This study aimed to investigate the effects of ASC-derived exosomes (ASC-EXOs) on human dermal fibroblasts (HDFs) and their potential combination with HA in in vivo wound healing and dermal filler models. In HDFs, ASC-EXOs increased cell proliferation and migration. ASC-EXOs also upregulated the expression of genes involved in cell proliferation and wound healing while stimulating collagen production in HDFs. In a porcine wound healing model, topical treatment with a combination of HA and ASC-EXOs led to higher wound closure rates compared to HA alone. Histological examination showed increased re-epithelialization and collagen type III deposition in wounds treated with the combination of HA and ASC-EXOs. In a mouse dermal filler model, tissues injected with the combination of HA and ASC-EXOs exhibited thicker tissue layers, increased vascularization, enhanced infiltration of myofibroblasts, and higher levels of collagen III and collagen fiber content compared to HA alone. These findings suggest that ASC-EXOs have beneficial effects on cell proliferation, migration, and gene expression related to wound healing, and they may accelerate wound closure and promote tissue regeneration. Furthermore, the combination of HA and ASC-EXOs may enhance wound healing and tissue remodeling, indicating its potential for both clinical and regenerative aesthetic applications in skin repair and regeneration.
Developmental Biology: Computational and Experimental Approaches
Authors: Ponomarenko Mikhail
Published: June 2023

Developmental biology studies ontogenesis, the individual development of an organism from the time of fertilization in sexual reproduction or its expelling from the maternal organism in asexual reproduction to the end of an organism’s life, with all phenotypical characters typical of this biological species and supporting the normal course of all biochemical processes and morphogenesis [...]
TRPM2 Channels: A Potential Therapeutic Target in Melanoma?
Authors: Foster Hattie M. , Carle McKenzie N. , Jira Lukas R. , Koh David W.
Published: June 2023

The transient receptor potential, the melastatin (TRPM) subfamily, which consists of eight known members, appears to have significant importance in melanoma progression, treatment, and prognosis. As several members were originally cloned from cancerous tissue, initial studies aimed towards identifying TRPM involvement in cancer progression and tumorigenesis. For relevance in skin cancer, previous research has shown roles for several TRPM members in skin cancer progression, growth, and patient prognosis. One unique member, TRPM2, appears to have notable therapeutic potential in the treatment of melanoma. Previous and recent studies have demonstrated increased TRPM2 expression levels in melanoma, as well as important roles for TRPM2 in melanoma growth, proliferation, and survival. TRPM2 is thus an emerging target in the treatment of melanoma, where TRPM2 antagonism may offer an additional treatment option for melanoma patients in the future.
Phytochemical, Morphological, and Physiological Variation in Different Ajowan (Trachyspermum ammi L.) Populations as Affected by Salt Stress, Genotype × Year Interaction and Pollination System
Authors: Mirniyam Gita , Rahimmalek Mehdi , Arzani Ahmad , Yavari Parisa , Sabzalian Mohammad R. , Ehtemam Mohammad Hossein , Szumny Antoni
Published: June 2023

In the present research, 28 populations of ajowan (Trachyspermum ammi L.) were evaluated for agro-morphological traits and essential oil yield in two consecutive years. Then, selected ajowan populations from these two years were used for further morphophysiological and biochemical studies under different salinity levels (control, 60, 90, and 120 mM NaCl). The main components of the oil were thymol (32.7–54.29%), γ-terpinene (21.71–32.81%), and p-cymene (18.74–26.16%). Salt stress caused an increase in essential oil content in the Esfahfo and Qazvin populations. The highest total phenolic and flavonoid contents were found in the Arak population grown in 60 mM NaCl (183.83 mg TAE g−1 DW) and the Yazd population grown in 90 mM NaCl (5.94 mg QE g−1 DW). Moreover, the Yazd population exhibited the strongest antioxidant activity based on DPPH (IC50 = 1566 µg/mL) under 60 mM NaCl and the highest reducing power (0.69 nm) under 120 mM NaCl. The results revealed that low and moderate salt stress improves the phytochemicals of ajowan seeds, which are useful for pharmaceutical and food applications. In this research, some morphological traits, as well as essential oil yield, were evaluated in open pollinated versus self-pollinated plants. As a result, plant height, number of flowering branches, and crown diameter significantly decreased in some populations, while a significant increase was obtained for number of flowers per umbel and seed numbers per umbel. Finally, self-pollination of ajowan might provide new insights for further breeding programs to increase oil or thymol content in ajowan.
APOE Locus-Associated Mitochondrial Function and Its Implication in Alzheimer’s Disease and Aging
Authors: Lee Eun-Gyung , Leong Lesley , Chen Sunny , Tulloch Jessica , Yu Chang-En
Published: June 2023

The Apolipoprotein E (APOE) locus has garnered significant clinical interest because of its association with Alzheimer’s disease (AD) and longevity. This genetic association appears across multiple genes in the APOE locus. Despite the apparent differences between AD and longevity, both conditions share a commonality of aging-related changes in mitochondrial function. This commonality is likely due to accumulative biological effects partly exerted by the APOE locus. In this study, we investigated changes in mitochondrial structure/function-related markers using oxidative stress-induced human cellular models and postmortem brains (PMBs) from individuals with AD and normal controls. Our results reveal a range of expressional alterations, either upregulated or downregulated, in these genes in response to oxidative stress. In contrast, we consistently observed an upregulation of multiple APOE locus genes in all cellular models and AD PMBs. Additionally, the effects of AD status on mitochondrial DNA copy number (mtDNA CN) varied depending on APOE genotype. Our findings imply a potential coregulation of APOE locus genes possibly occurring within the same topologically associating domain (TAD) of the 3D chromosome conformation. The coordinated expression of APOE locus genes could impact mitochondrial function, contributing to the development of AD or longevity. Our study underscores the significant role of the APOE locus in modulating mitochondrial function and provides valuable insights into the underlying mechanisms of AD and aging, emphasizing the importance of this locus in clinical research.
Hypoxia Preconditioned Serum (HPS) Promotes Proliferation and Chondrogenic Phenotype of Chondrocytes In Vitro
Authors: Jiang Jun , Altammar Jannat , Cong Xiaobin , Ramsauer Lukas , Steinbacher Vincent , Dornseifer Ulf , Schilling Arndt F. , Machens Hans-Günther , Moog Philipp
Published: June 2023

Autologous chondrocyte implantation (ACI) for the treatment of articular cartilage defects remains challenging in terms of maintaining chondrogenic phenotype during in vitro chondrocyte expansion. Growth factor supplementation has been found supportive in improving ACI outcomes by promoting chondrocyte redifferentiation. Here, we analysed the chondrogenic growth factor concentrations in the human blood-derived secretome of Hypoxia Preconditioned Serum (HPS) and assessed the effect of HPS-10% and HPS-40% on human articular chondrocytes from osteoarthritic cartilage at different time points compared to normal fresh serum (NS-10% and NS-40%) and FCS-10% culture conditions. In HPS, the concentrations of TGF-beta1, IGF-1, bFGF, PDGF-BB and G-CSF were found to be higher than in NS. Chondrocyte proliferation was promoted with higher doses of HPS (HPS-40% vs. HPS-10%) and longer stimulation (4 vs. 2 days) compared to FCS-10%. On day 4, immunostaining of the HPS-10%-treated chondrocytes showed increased levels of collagen type II compared to the other conditions. The promotion of the chondrogenic phenotype was validated with quantitative real-time PCR for the expression of collagen type II (COL2A1), collagen type I (COL1A1), SOX9 and matrix metalloproteinase 13 (MMP13). We demonstrated the highest differentiation index (COL2A1/COL1A1) in HPS-10%-treated chondrocytes on day 4. In parallel, the expression of differentiation marker SOX9 was elevated on day 4, with HPS-10% higher than NS-10/40% and FCS-10%. The expression of the cartilage remodelling marker MMP13 was comparable across all culture conditions. These findings implicate the potential of HPS-10% to improve conventional FCS-based ACI culture protocols by promoting the proliferation and chondrogenic phenotype of chondrocytes during in vitro expansion.
Pain Biomarkers in Fibromyalgia Syndrome: Current Understanding and Future Directions
Authors: Favretti Martina , Iannuccelli Cristina , Di Franco Manuela
Published: June 2023

Fibromyalgia is a complex and heterogeneous clinical syndrome, mainly characterized by the presence of widespread pain, possibly associated with a variety of other symptoms. Fibromyalgia can have an extremely negative impact on the psychological, physical and social lives of people affected, sometimes causing patients to experience dramatically impaired quality of life. Nowadays, the diagnosis of fibromyalgia is still clinical, thus favoring diagnostic uncertainties and making its clear identification challenging to establish, especially in primary care centers. These difficulties lead patients to undergo innumerable clinical visits, investigations and specialist consultations, thus increasing their stress, frustration and even dissatisfaction. Unfortunately, research over the last 25 years regarding a specific biomarker for the diagnosis of fibromyalgia has been fruitless. The discovery of a reliable biomarker for fibromyalgia syndrome would be a critical step towards the early identification of this condition, not only reducing patient healthcare utilization and diagnostic test execution but also providing early intervention with guideline-based treatments. This narrative article reviews different metabolite alterations proposed as possible biomarkers for fibromyalgia, focusing on their associations with clinical evidence of pain, and highlights some new, promising areas of research in this context. Nevertheless, none of the analyzed metabolites emerge as sufficiently reliable to be validated as a diagnostic biomarker. Given the complexity of this syndrome, in the future, a panel of biomarkers, including subtype-specific biomarkers, could be considered as an interesting alternative research area.
Elastomeric Porous Poly(glycerol sebacate) Methacrylate (PGSm) Microspheres as 3D Scaffolds for Chondrocyte Culture and Cartilage Tissue Engineering
Authors: Singh Dharaminder , Lindsay Sarah , Gurbaxani Shruti , Crawford Aileen , Claeyssens Frederik
Published: June 2023

Cartilage defects can be difficult to treat; therefore, tissue engineering of cartilage is emerging as a promising potential therapy. One interesting area of research explores the delivery of cells to the cartilage defect via scaffold-based cell delivery vehicles and microsurgery. This study explores the use of novel poly(glycerol sebacate) methacrylate (PGSm)-polymerised high internal phase emulsion (polyHIPE) microspheres as scaffolds with embedded cells for cartilage tissue engineering. Porous microsphere scaffolds (100 µm–1 mm diameter) were produced from emulsions consisting of water and a methacrylate-based photocurable resin of poly(glycerol sebacate). These resins were used in conjunction with a T-junction fluidic device and an ultraviolet (UV) curing lamp to produce porous microspheres with a tuneable size. This technique produced biodegradable PGSm microspheres with similar mechanical properties to cartilage. We further explore these microspheres as scaffolds for three-dimensional culture of chondrocytes. The microspheres proved to be very efficient scaffolds for primary chondrocyte culture and were covered by a dense extracellular matrix (ECM) network during the culture period, creating a tissue disk. The presence of glycosaminoglycans (GAGs) and collagen-II was confirmed, highlighting the utility of the PGSm microspheres as a delivery vehicle for chondrocytes. A number of imaging techniques were utilised to analyse the tissue disk and develop methodologies to characterise the resultant tissue. This study highlights the utility of porous PGSm microspheres for cartilage tissue engineering.
Emerging Role of GLP-1 Agonists in Obesity: A Comprehensive Review of Randomised Controlled Trials
Authors: Popoviciu Mihaela-Simona , Păduraru Lorena , Yahya Galal , Metwally Kamel , Cavalu Simona
Published: June 2023

Obesity is a chronic disease with high prevalence and associated comorbidities, making it a growing global concern. These comorbidities include type 2 diabetes, hypertension, ventilatory dysfunction, arthrosis, venous and lymphatic circulation diseases, depression, and others, which have a negative impact on health and increase morbidity and mortality. GLP-1 agonists, used to treat type 2 diabetes, have been shown to be effective in promoting weight loss in preclinical and clinical studies. This review summarizes numerous studies conducted on the main drugs in the GLP-1 agonists class, outlining the maximum achievable weight loss. Our aim is to emphasize the active role and main outcomes of GLP-1 agonists in promoting weight loss, as well as in improving hyperglycemia, insulin sensitivity, blood pressure, cardio–metabolic, and renal protection. We highlight the pleiotropic effects of these medications, along with their indications, contraindications, and precautions for both diabetic and non-diabetic patients, based on long-term follow-up studies.