bluuebunny/crossref_metadata_2025 huggingface dataset:
Altered mRNA Levels of Stress-Related Peptides in Mouse Hippocampus and Caudate-Putamen in Withdrawal after Long-Term Intermittent Exposure to Tobacco Smoke or Electronic Cigarette Vapour
Authors: Carboni Lucia , Ponzoni Luisa , Braida Daniela , Sala Mariaelvina , Gotti Cecilia , Zoli Michele
Published: January 2021

Nicotine addiction is a severe public health problem. The aim of this study was to investigate the alterations in key neurotransmissions after 60 days of withdrawal from seven weeks of intermittent cigarette smoke, e-cigarette vapours, or an e-cigarette vehicle. In the nicotine withdrawal groups, increased depressive and anxiety/obsessive–compulsive-like behaviours were demonstrated in the tail suspension, sucrose preference and marble burying tests. Cognitive impairments were detected in the spatial object recognition test. A significant increase in Corticotropin-releasing factor (Crf) and Crf1 mRNA levels was observed, specifically after cigarette withdrawal in the caudate-putamen nucleus (CPu). The nociceptin precursor levels were reduced by cigarette (80%) and e-cigarette (50%) withdrawal in the CPu. The delta opioid receptor showed a significant reduction in the hippocampus driven by the exposure to an e-cigarette solubilisation vehicle, while the mRNA levels doubled in the CPu of mice that had been exposed to e-cigarettes. Withdrawal after exposure to e-cigarette vapour induced a 35% Bdnf mRNA decrease in the hippocampus, whereas Bdnf was augmented by 118% by cigarette withdrawal in the CPu. This study shows that long-term withdrawal-induced affective and cognitive symptoms associated to lasting molecular alterations in peptidergic signalling may determine the impaired neuroplasticity in the hippocampal and striatal circuitry.
SAAFEC-SEQ: A Sequence-Based Method for Predicting the Effect of Single Point Mutations on Protein Thermodynamic Stability
Authors: Li Gen , Panday Shailesh Kumar , Alexov Emil
Published: January 2021

Modeling the effect of mutations on protein thermodynamics stability is useful for protein engineering and understanding molecular mechanisms of disease-causing variants. Here, we report a new development of the SAAFEC method, the SAAFEC-SEQ, which is a gradient boosting decision tree machine learning method to predict the change of the folding free energy caused by amino acid substitutions. The method does not require the 3D structure of the corresponding protein, but only its sequence and, thus, can be applied on genome-scale investigations where structural information is very sparse. SAAFEC-SEQ uses physicochemical properties, sequence features, and evolutionary information features to make the predictions. It is shown to consistently outperform all existing state-of-the-art sequence-based methods in both the Pearson correlation coefficient and root-mean-squared-error parameters as benchmarked on several independent datasets. The SAAFEC-SEQ has been implemented into a web server and is available as stand-alone code that can be downloaded and embedded into other researchers’ code.
Molecular Epidemiology of the Main Druggable Genetic Alterations in Non-Small Cell Lung Cancer
Authors: Fois Sara S. , Paliogiannis Panagiotis , Zinellu Angelo , Fois Alessandro G. , Cossu Antonio , Palmieri Giuseppe
Published: January 2021

Lung cancer is the leading cause of death for malignancy worldwide. Its molecular profiling has enriched our understanding of cancer initiation and progression and has become fundamental to provide guidance on treatment with targeted therapies. Testing the presence of driver mutations in specific genes in lung tumors has thus radically changed the clinical management and outcomes of the disease. Numerous studies performed with traditional sequencing methods have investigated the occurrence of such mutations in lung cancer, and new insights regarding their frequency and clinical significance are continuously provided with the use of last generation sequencing technologies. In this review, we discuss the molecular epidemiology of the main druggable genetic alterations in non-small cell lung cancer, namely EGFR, KRAS, BRAF, MET, and HER2 mutations or amplification, as well as ALK and ROS1 fusions. Furthermore, we investigated the predictive impact of these alterations on the outcomes of modern targeted therapies, their global prognostic significance, and their mutual interaction in cases of co-occurrence.
Mitochondrial Dysfunction and Heart Disease: Critical Appraisal of an Overlooked Association
Authors: Bisaccia Giandomenico , Ricci Fabrizio , Gallina Sabina , Di Baldassarre Angela , Ghinassi Barbara
Published: January 2021

The myocardium is among the most energy-consuming tissues in the body, burning from 6 to 30 kg of ATP per day within the mitochondria, the so-called powerhouse of the cardiomyocyte. Although mitochondrial genetic disorders account for a small portion of cardiomyopathies, mitochondrial dysfunction is commonly involved in a broad spectrum of heart diseases, and it has been implicated in the development of heart failure via maladaptive circuits producing and perpetuating mitochondrial stress and energy starvation. In this bench-to-bedside review, we aimed to (i) describe the key functions of the mitochondria within the myocardium, including their role in ischemia/reperfusion injury and intracellular calcium homeostasis; (ii) examine the contribution of mitochondrial dysfunction to multiple cardiac disease phenotypes and their transition to heart failure; and (iii) discuss the rationale and current evidence for targeting mitochondrial function for the treatment of heart failure, including via sodium-glucose cotransporter 2 inhibitors.
Effectiveness of Columbianadin, a Bioactive Coumarin Derivative, in Perturbing Transient and Persistent INa
Authors: Chang Wei-Ting , Wu Sheng-Nan
Published: January 2021

Columbianadin (CBN) is a bioactive coumarin-type compound with various biological activities. However, the action of CBN on the ionic mechanism remains largely uncertain, albeit it was reported to inhibit voltage-gated Ca2+ current or to modulate TRP-channel activity. In this study, whole-cell patch-clamp current recordings were undertaken to explore the modifications of CBN or other related compounds on ionic currents in excitable cells (e.g., pituitary GH3 cells and HL-1 atrial cardiomyocytes). GH3-cell exposure to CBN differentially decreased peak or late component of voltage-gated Na+ current (INa) with effective IC50 of 14.7 or 2.8 µM, respectively. The inactivation time course of INa activated by short depolarization became fastened in the presence of CBN with estimated KD value of 3.15 µM. The peak INa diminished by 10 µM CBN was further suppressed by subsequent addition of either sesamin (10 µM), ranolazine (10 µM), or tetrodotoxin (1 µM), but it was reversed by 10 µM tefluthrin (Tef); however, further application of 10 µM nimodipine failed to alter CBN-mediated inhibition of INa. CBN (10 µM) shifted the midpoint of inactivation curve of INa to the leftward direction. The CBN-mediated inhibition of peak INa exhibited tonic and use-dependent characteristics. Using triangular ramp pulse, the hysteresis of persistent INa enhanced by Tef was noticed, and the behavior was attenuated by subsequent addition of CBN. The delayed-rectifier or erg-mediated K+ current was mildly inhibited by 10 µM CBN, while it also slightly inhibited the amplitude of hyperpolarization-activated cation current. In HL-1 atrial cardiomyocytes, CBN inhibited peak INa and raised the inactivation rate of the current; moreover, further application of 10 µM Tef attenuated CBN-mediated decrease in INa. Collectively, this study provides an important yet unidentified finding revealing that CBN modifies INa in electrically excitable cells.
Mechanisms of Bone Fragility: From Osteogenesis Imperfecta to Secondary Osteoporosis
Authors: El-Gazzar Ahmed , Högler Wolfgang
Published: January 2021

Bone material strength is determined by several factors, such as bone mass, matrix composition, mineralization, architecture and shape. From a clinical perspective, bone fragility is classified as primary (i.e., genetic and rare) or secondary (i.e., acquired and common) osteoporosis. Understanding the mechanism of rare genetic bone fragility disorders not only advances medical knowledge on rare diseases, it may open doors for drug development for more common disorders (i.e., postmenopausal osteoporosis). In this review, we highlight the main disease mechanisms underlying the development of human bone fragility associated with low bone mass known to date. The pathways we focus on are type I collagen processing, WNT-signaling, TGF-ß signaling, the RANKL-RANK system and the osteocyte mechanosensing pathway. We demonstrate how the discovery of most of these pathways has led to targeted, pathway-specific treatments.
Upregulation of LAMB1 via ERK/c-Jun Axis Promotes Gastric Cancer Growth and Motility
Authors: Lee Hana , Kim Won-Jin , Kang Hyeon-Gu , Jang Jun-Ho , Choi Il Ju , Chun Kyung-Hee , Kim Seok-Jun
Published: January 2021

Gastric cancer is the fifth most common cancer worldwide with a poor survival rate. Therefore, it is important to identify predictive and prognostic biomarkers of gastric cancer. Laminin subunit beta 1 (LAMB1) is involved in attachment, migration, and organization during development, and its elevated expression has been associated with several cancers. However, the role and mechanism of LAMB1 in gastric cancer remains unknown. Here, we determined that LAMB1 is upregulated in gastric cancer tissues and contributes to cell growth and motility. Using a public database, we showed that LAMB1 expression was significantly upregulated in gastric cancer compared to normal tissues. LAMB1 was also found to be associated with poor prognosis in patients with gastric cancer. Overexpression of LAMB1 elevated cell proliferation, invasion, and migration; however, knockdown of LAMB1 decreased these effects in gastric cancer cells. U0126, an extracellular signal-regulated kinase (ERK) inhibitor, regulated the expression of LAMB1 in gastric cancer cells. Additionally, we showed that c-Jun directly binds to the LAMB1 promoter as a transcription factor and regulates its gene expression via the ERK pathway in gastric cancer cells. Therefore, our study indicates that LAMB1 promotes cell growth and motility via the ERK/c-Jun axis and is a potential biomarker and therapeutic target of gastric cancer.
Sirtuins as Important Factors in Pathological States and the Role of Their Molecular Activity Modulators
Authors: Kratz Ewa Maria , Sołkiewicz Katarzyna , Kubis-Kubiak Adriana , Piwowar Agnieszka
Published: January 2021

Sirtuins (SIRTs), enzymes from the family of NAD+-dependent histone deacetylases, play an important role in the functioning of the body at the cellular level and participate in many biochemical processes. The multi-directionality of SIRTs encourages scientists to undertake research aimed at understanding the mechanisms of their action and the influence that SIRTs have on the organism. At the same time, new substances are constantly being sought that can modulate the action of SIRTs. Extensive research on the expression of SIRTs in various pathological conditions suggests that regulation of their activity may have positive results in supporting the treatment of certain metabolic, neurodegenerative or cancer diseases or this connected with oxidative stress. Due to such a wide spectrum of activity, SIRTs may also be a prognostic markers of selected pathological conditions and prove helpful in assessing their progression, especially by modulating their activity. The article presents and discusses the activating or inhibiting impact of individual SIRTs modulators. The review also gathered selected currently available information on the expression of SIRTs in individual disease cases as well as the biological role that SIRTs play in the human organism, also in connection with oxidative stress condition, taking into account the progress of knowledge about SIRTs over the years, with particular reference to the latest research results.
Fission Yeast Methylenetetrahydrofolate Reductase Ensures Mitotic and Meiotic Chromosome Segregation Fidelity
Authors: Lim Kim Kiat , Teo Hwei Yee , Tan Yuan Yee , Zeng Yi Bing , Lam Ulysses Tsz Fung , Choolani Mahesh , Chen Ee Sin
Published: January 2021

Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in the folate metabolic pathway, and its loss of function through polymorphisms is often associated with human conditions, including cancer, congenital heart disease, and Down syndrome. MTHFR is also required in the maintenance of heterochromatin, a crucial determinant of genomic stability and precise chromosomal segregation. Here, we characterize the function of a fission yeast gene met11+, which encodes a protein that is highly homologous to the mammalian MTHFR. We show that, although met11+ is not essential for viability, its disruption increases chromosome missegregation and destabilizes constitutive heterochromatic regions at pericentromeric, sub-telomeric and ribosomal DNA (rDNA) loci. Transcriptional silencing at these sites were disrupted, which is accompanied by the reduction in enrichment of histone H3 lysine 9 dimethylation (H3K9me2) and binding of the heterochromatin protein 1 (HP1)-like Swi6. The met11 null mutant also dominantly disrupts meiotic fidelity, as displayed by reduced sporulation efficiency and defects in proper partitioning of the genetic material during meiosis. Interestingly, the faithful execution of these meiotic processes is synergistically ensured by cooperation among Met11, Rec8, a meiosis-specific cohesin protein, and the shugoshin protein Sgo1, which protects Rec8 from untimely cleavage. Overall, our results suggest a key role for Met11 in maintaining pericentromeric heterochromatin for precise genetic inheritance during mitosis and meiosis.
Iron Complexes of Flavonoids-Antioxidant Capacity and Beyond
Authors: Kejík Zdeněk , Kaplánek Robert , Masařík Michal , Babula Petr , Matkowski Adam , Filipenský Petr , Veselá Kateřina , Gburek Jakub , Sýkora David , Martásek Pavel , Jakubek Milan
Published: January 2021

Flavonoids are common plant natural products able to suppress ROS-related damage and alleviate oxidative stress. One of key mechanisms, involved in this phenomenon is chelation of transition metal ions. From a physiological perspective, iron is the most significant transition metal, because of its abundance in living organisms and ubiquitous involvement in redox processes. The chemical, pharmaceutical, and biological properties of flavonoids can be significantly affected by their interaction with transition metal ions, mainly iron. In this review, we explain the interaction of various flavonoid structures with Fe(II) and Fe(III) ions and critically discuss the influence of chelated ions on the flavonoid biochemical properties. In addition, specific biological effects of their iron metallocomplexes, such as the inhibition of iron-containing enzymes, have been included in this review.
Genetic Variations in Prostaglandin E2 Pathway Identified as Susceptibility Biomarkers for Gastric Cancer in an Intermediate Risk European Country
Authors: Lopes Catarina , Pereira Carina , Farinha Mónica , Medeiros Rui , Dinis-Ribeiro Mário
Published: January 2021

The cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2) pathway exerts deleterious pleiotropic effects in inflammation-induced gastric carcinogenesis. We aimed to assess the association of genetic variants in prostaglandin-endoperoxide synthase 2 (PTGS2), ATP binding cassette subfamily C member 4 (ABCC4), hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), and solute carrier organic anion transporter family member 2A1 (SLCO2A1) PGE2 pathway-related genes with gastric cancer (GC) risk in a European Caucasian population. A hospital-based case-control study gathering 260 GC cases and 476 cancer-free controls was implemented. Using a tagSNP approach, 51 single nucleotide polymorphisms (SNPs) were genotyped through MassARRAY® iPLEX Gold Technology or allelic discrimination by real-time polymerase chain reaction (PCR). Homozygous carriers of the minor allele for both rs689466 and rs10935090 SNPs were associated with a 2.98 and 4.30-fold increased risk for GC, respectively (95% confidence interval (CI): 1.14–7.74, p = 0.027; 95% CI: 1.22–15.16, p = 0.026), with the latter also being associated with an anticipated diagnosis age. A multifactor dimensionality reduction analysis identified an overall three-factor best interactive model composed of age, rs689466, and rs1678374 that was associated with a 17.6-fold GC increased risk (95% CI: 11.67–26.48, p < 0.0001, (cross-validation) CV consistency of 8/10 and accuracy of 0.807). In this preliminary study, several tagSNPs in PGE2 pathway-related genes were identified as risk biomarkers for GC development. This approach may help to identify higher-risk individuals and may contribute to the tailoring screening of GC in intermediate-risk European countries.
Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm
Authors: Zhang Xiaoning , Zhou Wenwen , Zhang Peng , Gao Fengxin , Zhao Xiuling , Shum Winnie Waichi , Zeng Xuhui
Published: January 2021

The calcium-binding protein spermatid-associated 1 (Cabs1) is a novel spermatid-specific protein. However, its function remains largely unknown. In this study, we found that a long noncoding RNA (lncRNA) transcripted from the Cabs1 gene antisense, AntiCabs1, was also exclusively expressed in spermatids. Cabs1 and AntiCabs1 knockout mice were generated separately (using Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-Cas9 methods) to investigate their functions in spermatogenesis. The genetic loss of Cabs1 did not affect testicular and epididymal development; however, male mice exhibited significantly impaired sperm tail structure and subfertility. Ultrastructural analysis revealed defects in sperm flagellar differentiation leading to an abnormal annulus and disorganization of the midpiece–principal piece junction, which may explain the high proportion of sperm with a bent tail. Interestingly, the proportion of sperm with a bent tail increased during transit in the epididymis. Furthermore, Western blot and immunofluorescence analyses showed that a genetic loss of Cabs1 decreased Septin 4 and Krt1 and increased cyclin Y-like 1 (Ccnyl1) levels compared with the wild type, suggesting that Cabs1 deficiency disturbed the expression of cytoskeleton-related proteins. By contrast, AntiCabs1−/− mice were indistinguishable from the wild type regarding testicular and epididymal development, sperm morphology, concentration and motility, and male fertility. This study demonstrates that Cabs1 is an important component of the sperm annulus essential for proper sperm tail assembly and motility.
Venous Thrombosis and Thrombocyte Activity in Zebrafish Models of Quantitative and Qualitative Fibrinogen Disorders
Authors: Fish Richard J. , Freire Cristina , Di Sanza Corinne , Neerman-Arbez Marguerite
Published: January 2021

Venous thrombosis occurs in patients with quantitative and qualitative fibrinogen disorders. Injury-induced thrombosis in zebrafish larvae has been used to model human coagulopathies. We aimed to determine whether zebrafish models of afibrinogenemia and dysfibrinogenemia have different thrombotic phenotypes. Laser injuries were used to induce venous thrombosis and the time-to-occlusion (TTO) and the binding and aggregation of fluorescent Tg(itga2b:EGFP) thrombocytes measured. The fga−/− larvae failed to support occlusive venous thrombosis and showed reduced thrombocyte binding and aggregation at injury sites. The fga+/− larvae were largely unaffected. When genome editing zebrafish to produce fibrinogen Aα R28C, equivalent to the human Aα R35C dysfibrinogenemia mutation, we detected in-frame skipping of exon 2 in the fga mRNA, thereby encoding AαΔ19–56. This mutation is similar to Fibrinogen Montpellier II which causes hypodysfibrinogenemia. Aα+/Δ19–56 fish had prolonged TTO and reduced thrombocyte activity, a dominant effect of the mutation. Finally, we used transgenic expression of fga R28C cDNA in fga knock-down or fga−/− mutants to model thrombosis in dysfibrinogenemia. Aα R28C expression had similar effects on TTO and thrombocyte activity as Aα+/Δ19–56. We conclude that thrombosis assays in larval zebrafish can distinguish between quantitative and qualitative fibrinogen disorder models and may assist in anticipating a thrombotic phenotype of novel fibrinogen mutations.
Effects of Survival Motor Neuron Protein on Germ Cell Development in Mouse and Human
Authors: Chang Wei-Fang , Peng Min , Hsu Jing , Xu Jie , Cho Huan-Chieh , Hsieh-Li Hsiu-Mei , Liu Ji-Long , Lu Chung-Hao , Sung Li-Ying
Published: January 2021

Survival motor neuron (SMN) is ubiquitously expressed in many cell types and its encoding gene, survival motor neuron 1 gene (SMN1), is highly conserved in various species. SMN is involved in the assembly of RNA spliceosomes, which are important for pre-mRNA splicing. A severe neurogenic disease, spinal muscular atrophy (SMA), is caused by the loss or mutation of SMN1 that specifically occurred in humans. We previously reported that SMN plays roles in stem cell biology in addition to its roles in neuron development. In this study, we investigated whether SMN can improve the propagation of spermatogonia stem cells (SSCs) and facilitate the spermatogenesis process. In in vitro culture, SSCs obtained from SMA model mice showed decreased growth rate accompanied by significantly reduced expression of spermatogonia marker promyelocytic leukemia zinc finger (PLZF) compared to those from heterozygous and wild-type littermates; whereas SMN overexpressed SSCs showed enhanced cell proliferation and improved potency. In vivo, the superior ability of homing and complete performance in differentiating progeny was shown in SMN overexpressed SSCs in host seminiferous tubule of transplant experiments compared to control groups. To gain insights into the roles of SMN in clinical infertility, we derived human induced pluripotent stem cells (hiPSCs) from azoospermia patients (AZ-hiPSCs) and from healthy control (ct-hiPSCs). Despite the otherwise comparable levels of hallmark iPCS markers, lower expression level of SMN1 was found in AZ-hiPSCs compared with control hiPSCs during in vitro primordial germ cell like cells (PGCLCs) differentiation. On the other hand, overexpressing hSMN1 in AZ-hiPSCs led to increased level of pluripotent markers such as OCT4 and KLF4 during PGCLC differentiation. Our work reveal novel roles of SMN in mammalian spermatogenesis and suggest new therapeutic targets for azoospermia treatment.
Ethical and Psychosocial Implications of Genomic Newborn Screening
Authors: Levy Harvey L.
Published: January 2021

The potential for genomic screening of the newborn, specifically adding genomic screening to current newborn screening (NBS), raises very significant ethical issues. Regardless of whether NBS of this type would include entire genomes or only the coding region of the genome (exome screening) or even sequencing specific genes, the ethical issues raised would be enormous. These issues include the limitations of bioinformatic interpretation of identified variants in terms of pathogenicity and accurate prognosis, the potential for substantial uncertainty about appropriate diagnosis, therapy, and follow-up, the possibility of much anxiety among providers and parents, the potential for unnecessary treatment and “medicalizing” normal children, the possibility of adding large medical costs for otherwise unnecessary follow-up and testing, the potential for negatively impacting medical and life insurance, and the almost impossible task of obtaining truly-informed consent. Moreover, the potentially-negative consequences of adding genomic sequencing to NBS might jeopardize all of NBS which has been and continues to be so beneficial for thousands of children and their families throughout the world.
Ethical Issues Surrounding Newborn Screening
Authors: Howell R. Rodney
Published: January 2021

It would be difficult to overestimate the importance of persistent, thoughtful parents and their importance in the development of treatments for their children’s rare disorders. Almost a century ago in Norway, observant parents led a brilliant young physician-scientist to his discovery of the underlying cause of their children’s profound developmental delay—i.e., phenylketonuria, or PKU. Decades later, in a recovering war-ravaged Britain, an equally persistent mother pressed the scientists at Birmingham Children’s Hospital to find a way to treat her seriously damaged daughter, Sheila, who suffered from PKU. Living on the financial edge, this mother insisted that Bickel and colleagues develop such a diet, and she volunteered Sheila to be the patient in the trial. The scientists concluded that the low phenylalanine diet helped but needed to be started very early—so, newborn screening was born to permit the implementation of this. Many steps brought us to where we are today, but these courageous parents made it all begin.
Combating Fake News in “Low-Resource” Languages: Amharic Fake News Detection Accompanied by Resource Crafting
Authors: Gereme Fantahun , Zhu William , Ayall Tewodros , Alemu Dagmawi
Published: January 2021

The need to fight the progressive negative impact of fake news is escalating, which is evident in the strive to do research and develop tools that could do this job. However, a lack of adequate datasets and good word embeddings have posed challenges to make detection methods sufficiently accurate. These resources are even totally missing for “low-resource” African languages, such as Amharic. Alleviating these critical problems should not be left for tomorrow. Deep learning methods and word embeddings contributed a lot in devising automatic fake news detection mechanisms. Several contributions are presented, including an Amharic fake news detection model, a general-purpose Amharic corpus (GPAC), a novel Amharic fake news detection dataset (ETH_FAKE), and Amharic fasttext word embedding (AMFTWE). Our Amharic fake news detection model, evaluated with the ETH_FAKE dataset and using the AMFTWE, performed very well.
Concept of an Ontology for Automated Vehicle Behavior in the Context of Human-Centered Research on Automated Driving Styles
Authors: Ossig Johannes , Cramer Stephanie , Bengler Klaus
Published: January 2021

In the human-centered research on automated driving, it is common practice to describe the vehicle behavior by means of terms and definitions related to non-automated driving. However, some of these definitions are not suitable for this purpose. This paper presents an ontology for automated vehicle behavior which takes into account a large number of existing definitions and previous studies. This ontology is characterized by an applicability for various levels of automated driving and a clear conceptual distinction between characteristics of vehicle occupants, the automation system, and the conventional characteristics of a vehicle. In this context, the terms ‘driveability’, ‘driving behavior’, ‘driving experience’, and especially ‘driving style’, which are commonly associated with non-automated driving, play an important role. In order to clarify the relationships between these terms, the ontology is integrated into a driver-vehicle system. Finally, the ontology developed here is used to derive recommendations for the future design of automated driving styles and in general for further human-centered research on automated driving.
A Frequent Pattern Conjunction Heuristic for Rule Generation in Data Streams
Authors: Stahl Frederic , Le Thien , Badii Atta , Gaber Mohamed Medhat
Published: January 2021

This paper introduces a new and expressive algorithm for inducing descriptive rule-sets from streaming data in real-time in order to describe frequent patterns explicitly encoded in the stream. Data Stream Mining (DSM) is concerned with the automatic analysis of data streams in real-time. Rapid flows of data challenge the state-of-the art processing and communication infrastructure, hence the motivation for research and innovation into real-time algorithms that analyse data streams on-the-fly and can automatically adapt to concept drifts. To date, DSM techniques have largely focused on predictive data mining applications that aim to forecast the value of a particular target feature of unseen data instances, answering questions such as whether a credit card transaction is fraudulent or not. A real-time, expressive and descriptive Data Mining technique for streaming data has not been previously established as part of the DSM toolkit. This has motivated the work reported in this paper, which has resulted in developing and validating a Generalised Rule Induction (GRI) tool, thus producing expressive rules as explanations that can be easily understood by human analysts. The expressiveness of decision models in data streams serves the objectives of transparency, underpinning the vision of ‘explainable AI’ and yet is an area of research that has attracted less attention despite being of high practical importance. The algorithm introduced and described in this paper is termed Fast Generalised Rule Induction (FGRI). FGRI is able to induce descriptive rules incrementally for raw data from both categorical and numerical features. FGRI is able to adapt rule-sets to changes of the pattern encoded in the data stream (concept drift) on the fly as new data arrives and can thus be applied continuously in real-time. The paper also provides a theoretical, qualitative and empirical evaluation of FGRI.
Ensemble and Quick Strategy for Searching Reduct: A Hybrid Mechanism
Authors: Yan Wangwang , Chen Yan , Shi Jinlong , Yu Hualong , Yang Xibei
Published: January 2021

Attribute reduction is commonly referred to as the key topic in researching rough set. Concerning the strategies for searching reduct, though various heuristics based forward greedy searchings have been developed, most of them were designed for pursuing one and only one characteristic which is closely related to the performance of reduct. Nevertheless, it is frequently expected that a justifiable searching should explicitly involves three main characteristics: (1) the process of obtaining reduct with low time consumption; (2) generate reduct with high stability; (3) acquire reduct with competent classification ability. To fill such gap, a hybrid based searching mechanism is designed, which takes the above characteristics into account. Such a mechanism not only adopts multiple fitness functions to evaluate the candidate attributes, but also queries the distance between attributes for determining whether two or more attributes can be added into the reduct simultaneously. The former may be useful in deriving reduct with higher stability and competent classification ability, and the latter may contribute to the lower time consumption of deriving reduct. By comparing with 5 state-of-the-art algorithms for searching reduct, the experimental results over 20 UCI data sets demonstrate the effectiveness of our new mechanism. This study suggests a new trend of attribute reduction for achieving a balance among various characteristics.
Impact of Sudden Global Events on Cross-Field Research Cooperation
Authors: Dang Zhongkai , Li Lixiang , Peng Haipeng , Zhang Jiaxuan
Published: January 2021

Since the outbreak of COVID-19, in addition to the continuous increment in the number of infected patients, the number of COVID-19-related papers has also increased significantly. According to the statistics, its number even exceeds the research of some research fields over many years. Similar to COVID-19, the related research on COVID-19 also seems highly infectious. What causes this situation? By crawling the data of COVID-19-related papers from web of Sciences this year, we found that there are three mechanisms to promote the rapid growth of the number of COVID-19 papers: incentive mechanism, cross-field collaboration mechanism, and potential impact mechanism of writing papers. To understand the impact of COVID-19 on cross-domain paper network further, we proposed a new construction method of multi-field paper association structure network based on COVID-19. The paper association mechanism and the wall breaking principle between multiple research fields were found through the experiments. Then, combined with the constructed network, we gave the knowledge dissemination model of the new discoveries in multiple fields and obtained some relevant new findings.
The Overview of the Conservation and Renewal of the Industrial Belgian Heritage as a Vector for Cultural Regeneration
Authors: Zhang Jiazhen , Cenci Jeremy , Becue Vincent , Koutra Sesil
Published: January 2021

Industrial heritage reflects the development track of human production activities and witnessed the rise and fall of industrial civilization. As one of the earliest countries in the world to start the Industrial Revolution, Belgium has a rich industrial history. Over the past years, a set of industrial heritage renewal projects have emerged in Belgium in the process of urban regeneration. In this paper, we introduce the basic contents of the related terms of industrial heritage, examine the overall situation of protection and renewal in Belgium. The industrial heritage in Belgium shows its regional characteristics, each region has its representative industrial heritage types. In the Walloon region, it is the heavy industry. In Flanders, it is the textile industry. In Brussels, it is the service industry. The kinds of industrial heritages in Belgium are coordinate with each other. Industrial heritage tourism is developed, especially on eco-tourism, experience tourism. The industrial heritage in transportation and mining are the representative industrial heritages in Belgium. There are a set of numbers industrial heritages are still in running based on a successful reconstruction into industrial tourism projects. Due to the advanced experience in dealing with industrial heritage, the industrial heritage and the city live together harmoniously.
Sensitivity Assessment of the Seismic Response of a Masonry Palace via Non-Linear Static Analysis: A Case Study in L’Aquila (Italy)
Authors: Capanna Ilaria , Aloisio Angelo , Di Fabio Franco , Fragiacomo Massimo
Published: January 2021

The city of L’Aquila (Italy) includes a significant amount of masonry palaces erected from the middle of the 13th century up to the first half of the 20th century. This paper focuses on the seismic response of a masonry palace built during the first half of the 20th century and characterized by regularity in plan and elevation. The authors investigate the seismic response by varying a suite of modelling parameters that express the actual scatter of the mechanical properties typical of the masonry palaces erected in L’Aquila. The authors discuss the seismic performance exhibited by this building during the 2009 earthquake. Then, they assess the sensitivity of the selected building’s seismic performance via non-linear static analysis to the mechanical properties of masonry, the in-plane stiffness of the floors, and the mechanical resistance of the spandrels. The parametric analysis shows that the three variables markedly affect the shear resistance, the ultimate displacement, and the behavior factors. The fragility functions were then estimated from the results of non-linear static analysis. A significant scatter of the probability of collapse for the considered limit states reveals the limitations of typological approaches for masonry palaces.
Pathogenicity and Side Effect of Indigenous Beauveria bassiana on Coccinella undecimpunctata and Hippodamia variegata (Coleoptera: Coccinellidae)
Authors: Sayed Samy , Elarrnaouty Sayed-Ashraf , AlOtaibi Saad , Salah Mohamed
Published: January 2021

This study aimed to estimate the virulence of an indigenous Beauveria bassiana on all developmental stages of two indigenous coccinellids; Coccinella undecimpunctata and Hippodamia variegata through three application methods; direct spray, contact method, and feeding on aphids treated with the fungus (ingestion). Also, indirect effect on all developmental stages resulted from 1st larval instar treated with these application methods. All treatments were done with a concentration of 1 × 105 which was recommended in previous studies for different aphid species with a control of 0.02% Tween 80 (v/v). The mortality of 1st larval instar of both H. variegata and C. undecimpunctata and pupal stage of C. undecimpunctata were significantly increased with spray method only. Also, contact method achieved significantly higher mortality on 1st larval instar of C. undecimpunctata only. Regard to indirect effect, except of mortality of 1st larval instar of both predators and 2nd larval instar of H. variegata, other developmental instars/stages of both predators were not affected by B. bassiana through the three tested application methods in the mortality, duration, survival, cumulative survival male and female longevity, and fecundity. Therefore, both tested predatory coccinellids could be compatible with this indigenous isolate of B. bassiana where, in general, there are no negative effects of the fungus on both predators.
Alarm Pheromone Responses Depend on Genotype, but Not on the Presence of Facultative Endosymbionts in the Pea Aphid Acyrthosiphon pisum
Authors: Badji Cesar Auguste , Sol-Mochkovitch Zoé , Fallais Charlotte , Sochard Corentin , Simon Jean-Christophe , Outreman Yannick , Anton Sylvia
Published: January 2021

Aphids use an alarm pheromone, E-β farnesene (EBF), to warn conspecifics of potential danger. The antennal sensitivity and behavioural escape responses to EBF can be influenced by different factors. In the pea aphid, Acyrthosiphon pisum, different biotypes are adapted to different legume species, and within each biotype, different genotypes exist, which can carry or not Hamiltonella defensa, a bacterial symbiont that can confer protection against natural enemies. We investigate here the influence of the aphid genotype and symbiotic status on the escape behaviour using a four-way olfactometer and antennal sensitivity for EBF using electroantennograms (EAGs). Whereas the investigated three genotypes from two biotypes showed significantly different escape and locomotor behaviours in the presence of certain EBF doses, the infection with H. defensa did not significantly modify the escape behaviour and only marginally influenced the locomotor behaviour at high doses of EBF. Dose-response curves of EAG amplitudes after stimulation with EBF differed significantly between aphid genotypes in correlation with behavioural differences, whereas antennal sensitivity to EBF did not change significantly as a function of the symbiotic status. The protective symbiont H. defensa does thus not modify the olfactory sensitivity to the alarm pheromone. How EBF sensitivity is modified between genotypes or biotypes remains to be investigated.
Hymenopteran Parasitoids of Aphid Pests within Australian Grain Production Landscapes
Authors: Ward Samantha E. , Umina Paul A. , Macfadyen Sarina , Hoffmann Ary A.
Published: January 2021

In grain crops, aphids are important pests, but they can be suppressed by hymenopteran parasitoids. A challenge in incorporating parasitoids into Integrated Pest Management (IPM) programs, however, is that parasitoid numbers can be low during periods within the season when aphids are most damaging. Understanding the population dynamics of key aphid species and their parasitoids is central to ameliorating this problem. To examine the composition and seasonal trends of both aphid and parasitoid populations in south-eastern Australia, samples were taken throughout the winter growing seasons of 2017 and 2018 in 28 fields of wheat and canola. Myzus persicae (Sulzer) was the most abundant aphid species, particularly within canola crops. Across all fields, aphid populations remained relatively low during the early stages of crop growth and increased as the season progressed. Seasonal patterns were consistent across sites, due to climate, crop growth stage, and interactions between these factors. For canola, field edges did not appear to act as reservoirs for either aphids or parasitoids, as there was little overlap in the community composition of either, but for wheat there was much similarity. This is likely due to the presence of similar host plants within field edges and the neighbouring crop, enabling the same aphid species to persist within both areas. Diaeretiella rapae (M’Intosh) was the most common parasitoid across our study, particularly in canola, yet was present only in low abundance at field edges. The most common parasitoid in wheat fields was Aphidius matricariae (Haliday), with field edges likely acting as a reservoir for this species. Secondary parasitoid numbers were consistently low across our study. Differences in parasitoid species composition are discussed in relation to crop type, inter-field variation, and aphid host. The results highlight potential focal management areas and parasitoids that could help control aphid pests within grain crops.
Biodiversity and Spatiotemporal Variation of Longhorn Beetles (Coleoptera: Cerambycidae) in Tropical Forest of Thailand
Authors: Yotkham Sirapat , Suttiprapan Piyawan , Likhitrakarn Natdanai , Sulin Chayanit , Srisuka Wichai
Published: January 2021

Longhorn beetles are highly diversified and important for agriculture and health of the environment. However, the fauna and ecology of these beetles are not well known in Thailand. This study is the first to report the biodiversity, elevation, and seasonal distribution of longhorn beetles. Specimens were collected by malaise traps from 41 localities in 24 national parks throughout the country during 2006–2009. The traps were operated at each site for 12 consecutive months with a monthly service. A total of 199 morphotaxa in 36 tribes of 6 subfamilies were identified from 1376 specimens. Of these, 40.7% and 14.5% of total taxa were singletons and doubletons, respectively. The Shannon diversity index and observed species richness at Panernthung, Loei Forest Unit and Mae Fang Hotspring were high at 0.96 (30), 0.88 (50), and 0.86 (34), respectively. Local richness ranged between 3 and 50 species, while the species richness estimator showed between 6 and 1275 species. The most relatively abundant species, Nupserha lenita, Pterolophia sp.1, Oberea sp.3, Acalolepta pseudospeciosa, and Ac. rustricatrix represented 4.80%, 4.80%, 4.80%, 4.5%, and 4.43% of the species, respectively. The species with the widest distribution range of percentage of species occurrence (% SO) was Pt. sp.1 (63.4%), followed by Ac. rustricatrix (39%) and Moechotypa suffusa (39%). In a significantly negative relationship between species richness and elevation (p > 0.05, R2 = 0.04), the species richness pattern showed a hump-shaped curve that peaked at the middle elevation (501–1000 m asl). Regarding seasonal variation, most of the species occurred during the hot season (March–April) and peaked in early rainy season (May), while a low number of species were found during the mid-rainy (June–October) and cold season (November–February). Ordination analysis indicated that the distribution of most species was associated with regions and forest type, and most of the species correlated with forest located at middle and low elevation. The results of this study indicated the very high biodiversity of longhorn beetles in Thailand, which suggests that an understanding of their seasonal and elevational distribution will be of value to agriculture management and conservation. They also indicated that malaise traps are appropriate for the evaluation of biodiversity.
Monitoring Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) Infestation in Soybean by Proximal Sensing
Authors: Barros Pedro P. S. , Schutze Inana X. , Iost Filho Fernando H. , Yamamoto Pedro T. , Fiorio Peterson R. , Demattê José A. M.
Published: January 2021

Although monitoring insect pest populations in the fields is essential in crop management, it is still a laborious and sometimes ineffective process. Imprecise decision-making in an integrated pest management program may lead to ineffective control in infested areas or the excessive use of insecticides. In addition, high infestation levels may diminish the photosynthetic activity of soybean, reducing their development and yield. Therefore, we proposed that levels of infested soybean areas could be identified and classified in a field using hyperspectral proximal sensing. Thus, the goals of this study were to investigate and discriminate the reflectance characteristics of soybean non-infested and infested with Bemisia tabaci using hyperspectral sensing data. Therefore, cages were placed over soybean plants in a commercial field and artificial whitefly infestations were created. Later, samples of infested and non-infested soybean leaves were collected and transported to the laboratory to obtain the hyperspectral curves. The results allowed us to discriminate the different levels of infestation and to separate healthy from whitefly infested soybean leaves based on their reflectance. In conclusion, these results show that hyperspectral sensing can potentially be used to monitor whitefly populations in soybean fields.
Factors Affecting Thanatosis in the Braconid Parasitoid Wasp Heterospilus prosopidis
Authors: Amemiya Mio , Sasakawa Kôji
Published: January 2021

Thanatosis, also called death feigning, is often an antipredator behavior. In insects, it has been reported from species of various orders, but knowledge of this behavior in Hymenoptera is insufficient. This study examined the effects of sex, age (0 or 2 days old), temperature (18 or 25 °C), and background color (white, green, or brown) on thanatosis in the braconid parasitoid wasp Heterospilus prosopidis. Thanatosis was more frequent in 0-d-old individuals and in females at 18 °C. The duration of thanatosis was longer in females, but this effect of sex was weaker at 18 °C and in 0-d-old individuals. The background color affected neither the frequency nor duration. These results were compared with reports for other insects and predictions based on the life history of this species, and are discussed from an ecological perspective.
Morphology of the Novel Basimandibular Gland in the Ant Genus Strumigenys (Hymenoptera, Formicidae)
Authors: Wang Chu , Steenhuyse-Vandevelde Michael , Lin Chung-Chi , Billen Johan
Published: January 2021

In 1999, Barry Bolton postulated the presence of a basimandibular gland in the mandibles in all species of the ant genus Strumigenys, solely based on scanning microscopy observations. We now confirm the presence of this putative gland in the proximal outer part of the mandibles of 22 investigated species by histological and ultrastructural examination, including 10 short- and 12 long-mandibulate species. All species have a basimandibular gland, that is formed by 15–25 µm thick epithelial cells and belongs to class-1 following the standard classification of insect exocrine glands. We consider it a novel gland because of its peculiar bowl-shape and special arrangement of the microvilli that are confined to large vacuolar spaces instead of reaching the cuticle. The gland is most pronounced in S. mutica, particularly in the queen. In addition to this gland, we also found scattered class-3 intramandibular gland cells in the mandibles. Queens of S. mutica are peculiar in having a cluster of these cells in the distal tip of their mandibles. As this species is a social parasite, further research is required to determine whether the development of these mandibular glands is related to its parasitic lifestyle.
Ecology and Biology of Aquatic Insects
Authors: Starr Scott M. , Wallace John R.
Published: January 2021

The advancement of our knowledge on the ecology and biology of aquatic insects is essential to improving our understanding of their roles in water quality, disease ecology, as indicators of climate change, biodiversity, as well as community structure and ecosystem functioning [...]