bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of racial disparities and insurance status in patients with bone sarcomas in the USA
Authors: Hu Xianglin , Fujiwara Tomohiro , Houdek Matthew T. , Chen Lingxiao , Huang Wending , Sun Zhengwang , Sun Yangbai , Yan Wangjun
Published: May 2022

Aims

Socioeconomic and racial disparities have been recognized as impacting the care of patients with cancer, however there are a lack of data examining the impact of these disparities on patients with bone sarcoma. The purpose of this study was to examine socioeconomic and racial disparities that impact the oncological outcomes of patients with bone sarcoma.

Methods

We reviewed 4,739 patients diagnosed with primary bone sarcomas from the Surveillance, Epidemiology and End Results (SEER) registry between 2007 and 2015. We examined the impact of race and insurance status associated with the presence of metastatic disease at diagnosis, treatment outcome, and overall survival (OS).

Results

Patients with Medicaid (odds ratio (OR) 1.41; 95% confidence interval (CI) 1.15 to 1.72) and uninsured patients (OR 1.90; 95% CI 1.26 to 2.86) had higher risks of metastatic disease at diagnosis compared to patients with health insurance. Compared to White patients, Black (OR 0.63, 95% CI 0.47 to 0.85) and Asian/Pacific Islander (OR 0.65, 95% CI 0.46 to 0.91) were less likely to undergo surgery. In addition, Black patients were less likely to receive chemotherapy (OR 0.67, 95% CI 0.49 to 0.91) compared to White patients. In patients with chondrosarcoma, those with Medicaid had worse OS compared to patients with insurance (hazard ratio (HR) 1.65, 95% CI 1.06 to 2.56).

Conclusion

In patients with a bone sarcoma, the cancer stage at diagnosis varied based on insurance status, and racial disparities were identified in treatment. Further studies are needed to identify modifiable factors which can mitigate socioeconomic and racial disparities found in patients with bone sarcomas. Cite this article: Bone Joint Res 2022;11(5):278–291.
Household Characteristics and Energy Provision of Rural Households in Central Nepal
Authors: Pariyar Bishnu , Rijal Bishnu , Lovett Jon C. , Bisunke Rozy , Shrestha Krishna Kumar
Published: May 2022

Access to affordable, reliable and modern energy services is the primary aim of Sustainable Development Goal 7 and underpins other SDGs for improving health and welfare. In keeping with the SDG objectives, national governments and development agencies have been advocating reduction of households’ dependency on traditional sources of energy and encourage the transition to clean energy. Whilst policy interventions for this have had some success, a large proportion of households, especially in the rural areas of developing countries still continue to face energy shortages and lack of access to clean energy continues to be a major developmental challenge. This study investigates the energy use of 295 rural households in three rural villages in central Nepal. Results demonstrate that households continue to depend on traditional sources of energy with limited access to clean energy. Furthermore, access to clean energy amongst households in rural Nepal is determined by households and community socio-economic status. The paper argues that much more needs to be done to enhance households’ access to clean energy and reduce their dependency on traditional sources of energy and the distributional implications of such measures need to be properly considered.
Thermal Energy Feedback Regulation of Human Life System Based on Photon Radiation
Authors: Jin Qinghong , Liu Zhan
Published: May 2022

To solve the problem of the long execution time of the traditional thermalenergy feedback regulation mechanism of human life system, a method ofanalyzing the thermal energy feedback regulation mechanism of human lifesystem based on photonic radiation was proposed. The energy responseentropy of human living system is calculated by analyzing the change ofhuman living system’s thermal energy. The photonic counter is designedby using photonic radiation technology to extract the weak optical signal ofthermal energy of the system and output energy accumulation. The quadraticprogramming algorithm is used to solve the optimal solution of thermalenergy, and the neural network model is combined with the energy accu-mulation of photon counter to realize the thermal feedback regulation. So far,the design of energy feedback regulation mechanism of human living systemhas been completed. The experimental results show that compared withthe traditional feedback control mechanism, the designed energy feedbackcontrol mechanism based on photonic radiation has a shorter implementationtime and is suitable for practical engineering.
Study on Water Regulation of Yong’an River Basin Based on Ecological Flow Guarantee
Authors: Guo Huifang , Yin Fangfang , Ma Haibo , Tian Chuanchong , Zhang Shixia
Published: May 2022

Water resources are the necessary guarantee to support the development of human society and one of the most important resources to maintain the health and stability of the ecological environment. With the gradual increase of China’s population and the gradual increase of water consumption for industry, life and agriculture, the water consumption for ecological environment can not be met. As a result, some rivers have dried up, biological habitat has been lost and species diversity has been reduced. Therefore, how to ensure the river ecological flow is a very urgent problem to be solved. This paper selects Yong’an River Basin as the research object. Yong’an river is a mountainous river with the characteristics of uneven distribution of natural runoff throughout the year and seasonal water shortage. At present, the ecological flow demand of the river has not been considered in the reservoir operation. In this paper, four different scheduling schemes are set up according to the two constraints of ecological base flow and target ecological flow. Finally, the reservoir group operation scheme with better water supply assurance rate and ecological flow assurance rate is determined.
Laser radius measurement method based on the amplitude jump of a
thermal wave
Authors: Hu Dongjin , Qin Xunpeng , Hu Zeqi , Zeng Yan , Ji Feilong
Published: May 2022

The laser radius should be calculated with high precision when analyzing the
characteristics of a Lamb wave excited by a laser in a thin plate.
However, traditional methods for measuring the radius of a laser beam
are complex. The paper aims to propose a novel convenient method, to
the best of our knowledge, for measuring the radius of a laser
ultrasonic beam. A laser interferometer (receiving laser) is used to
receive ultrasonic signals excited by the laser to be measured
(exciting laser) on the surface of a test block. Considering the
characteristics represented by the thermal wave, positions where the
receiving beam contacts and separates from the exciting beam are
determined, and the spot of the radius is calculated by corresponding
geometric relations. Experiments show that the accuracy of the
proposed method is in the order of magnitude of 0.01 mm, and the
relative errors of experimental data are within 2%. This paper
provides an alternative method for the measurement of the laser beam
radius and has great significance for the application of laser
ultrasonic detection in the thin plate and the calculation and
analysis of the dispersion curve.
Generation of a coherent distributed RF array with a strong positive correlation
Authors: Lu Yiwen , Jiang Zhongze , Wan Zhengyi , Yin Feifei , Xu Kun , Dai Yitang
Published: May 2022

In this work, we present a coherent distributed radio frequency (RF) array, discover and quantitatively describe the strong positive correlation between reconstructed signals for the first time. Eight replicable parallel receivers are connected to the phase-locked common trunk link via eight optical couplers spaced 1 km apart. The forward and backward signals at each receiver, extracted from two ports of optical couplers, are recovered to RF signals separately and then mixed to achieve upward frequency conversion. The link delay jitter is counteracted by wavelength-tuning of the optical carrier. With the long-term stability of point-to-multipoint fiber-optic RF dissemination effectively improved, the coherent distributed array is generated, and further the relative frequency stability between signals at different receivers is studied. The proposed correlation coefficient at 103 s is ∼0.8 and shows a slight downward trend with the increase of averaging time based on our experimental results.
Lensfree on-chip microscopy based on single-plane phase retrieval
Authors: Guo Cheng , Liu Xianming , Zhang Feilong , Du Yongbin , Zheng Shenghao , Wang Zehua , Zhang Xiaoqing , Kan Xingchi , Liu Zhengjun , Wang Weibo
Published: May 2022

We propose a novel single-plane phase retrieval method to realize high-quality sample reconstruction for lensfree on-chip microscopy. In our method, complex wavefield reconstruction is modeled as a quadratic minimization problem, where total variation and joint denoising regularization are designed to keep a balance of artifact removal and resolution enhancement. In experiment, we built a 3D-printed field-portable platform to validate the imaging performance of our method, where resolution chart, dynamic target, transparent cell, polystyrene beads, and stained tissue sections are employed for the imaging test. Compared to state-of-the-art methods, our method eliminates image degradation and obtains a higher imaging resolution. Different from multi-wavelength or multi-height phase retrieval methods, our method only utilizes a single-frame intensity data record to accomplish high-fidelity reconstruction of different samples, which contributes a simple, robust, and data-efficient solution to design a resource-limited lensfree on-chip microscope. We believe that it will become a useful tool for telemedicine and point-of-care application.
Pose correction scheme for camera-scanning Fourier ptychography based on camera calibration and homography transform
Authors: Cui Baiqi , Zhang Shaohui , Wang Yechao , Hu Yao , Hao Qun
Published: May 2022

Fourier ptychography (FP), as a computational imaging method, is a powerful tool to improve imaging resolution. Camera-scanning Fourier ptychography extends the application of FP from micro to macro creatively. Due to the non-ideal scanning of the camera driven by the mechanical translation stage, the pose error of the camera occurs, greatly degrading the reconstruction quality, while a precise translation stage is expensive and not suitable for wide-range imaging. Here, to improve the imaging performance of camera-scanning Fourier ptychography, we propose a pose correction scheme based on camera calibration and homography transform approaches. The scheme realizes the accurate alignment of data set and location error correction in the frequency domain. Simulation and experimental results demonstrate this method can optimize the reconstruction results and realize high-quality imaging effectively. Combined with the feature recognition algorithm, the scheme provides the possibility for applying FP in remote sensing imaging and space imaging.
Capturing the songs of mice with an improved detection and classification method for ultrasonic vocalizations (BootSnap)
Authors: Abbasi Reyhaneh , Balazs Peter , Marconi Maria Adelaide , Nicolakis Doris , Zala Sarah M. , Penn Dustin J.
Published: May 2022

House mice communicate through ultrasonic vocalizations (USVs), which are above the range of human hearing (>20 kHz), and several automated methods have been developed for USV detection and classification. Here we evaluate their advantages and disadvantages in a full, systematic comparison, while also presenting a new approach. This study aims to 1) determine the most efficient USV detection tool among the existing methods, and 2) develop a classification model that is more generalizable than existing methods. In both cases, we aim to minimize the user intervention required for processing new data. We compared the performance of four detection methods in an out-of-the-box approach, pretrained DeepSqueak detector, MUPET, USVSEG, and the Automatic Mouse Ultrasound Detector (A-MUD). We also compared these methods to human visual or ‘manual’ classification (ground truth) after assessing its reliability. A-MUD and USVSEG outperformed the other methods in terms of true positive rates using default and adjusted settings, respectively, and A-MUD outperformed USVSEG when false detection rates were also considered. For automating the classification of USVs, we developed BootSnap for supervised classification, which combines bootstrapping on Gammatone Spectrograms and Convolutional Neural Networks algorithms with Snapshot ensemble learning. It successfully classified calls into 12 types, including a new class of false positives that is useful for detection refinement. BootSnap outperformed the pretrained and retrained state-of-the-art tool, and thus it is more generalizable. BootSnap is freely available for scientific use.
Structural dynamics of SARS-CoV-2 nucleocapsid protein induced by RNA binding
Authors: Ribeiro-Filho Helder Veras , Jara Gabriel Ernesto , Batista Fernanda Aparecida Heleno , Schleder Gabriel Ravanhani , Costa Tonoli Celisa Caldana , Soprano Adriana Santos , Guimarães Samuel Leite , Borges Antonio Carlos , Cassago Alexandre , Bajgelman Marcio Chaim , Marques Rafael Elias , Trivella Daniela Barretto Barbosa , Franchini Kleber Gomes , Figueira Ana Carolina Migliorini , Benedetti Celso Eduardo , Lopes-de-Oliveira Paulo Sergio
Published: May 2022

The nucleocapsid (N) protein of the SARS-CoV-2 virus, the causal agent of COVID-19, is a multifunction phosphoprotein that plays critical roles in the virus life cycle, including transcription and packaging of the viral RNA. To play such diverse roles, the N protein has two globular RNA-binding modules, the N- (NTD) and C-terminal (CTD) domains, which are connected by an intrinsically disordered region. Despite the wealth of structural data available for the isolated NTD and CTD, how these domains are arranged in the full-length protein and how the oligomerization of N influences its RNA-binding activity remains largely unclear. Herein, using experimental data from electron microscopy and biochemical/biophysical techniques combined with molecular modeling and molecular dynamics simulations, we show that, in the absence of RNA, the N protein formed structurally dynamic dimers, with the NTD and CTD arranged in extended conformations. However, in the presence of RNA, the N protein assumed a more compact conformation where the NTD and CTD are packed together. We also provided an octameric model for the full-length N bound to RNA that is consistent with electron microscopy images of the N protein in the presence of RNA. Together, our results shed new light on the dynamics and higher-order oligomeric structure of this versatile protein.
The tomato yellow leaf curl virus C4 protein alters the expression of plant developmental genes correlating to leaf upward cupping phenotype in tomato
Authors: Padmanabhan Chellappan , Zheng Yi , Shamimuzzaman Md , Wilson Jennifer R. , Gilliard Andrea , Fei Zhangjun , Ling Kai-Shu
Published: May 2022

Tomato yellow leaf curl virus (TYLCV), a monopartite begomovirus in the family Geminiviridae, is efficiently transmitted by the whitefly, Bemisia tabaci, and causes serious economic losses to tomato crops around the world. TYLCV-infected tomato plants develop distinctive symptoms of yellowing and leaf upward cupping. In recent years, excellent progress has been made in the characterization of TYLCV C4 protein function as a pathogenicity determinant in experimental plants, including Nicotiana benthamiana and Arabidopsis thaliana. However, the molecular mechanism leading to disease symptom development in the natural host plant, tomato, has yet to be characterized. The aim of the current study was to generate transgenic tomato plants expressing the TYLCV C4 gene and evaluate differential gene expression through comparative transcriptome analysis between the transgenic C4 plants and the transgenic green fluorescent protein (Gfp) gene control plants. Transgenic tomato plants expressing TYLCV C4 developed phenotypes, including leaf upward cupping and yellowing, that are similar to the disease symptoms expressed on tomato plants infected with TYLCV. In a total of 241 differentially expressed genes identified in the transcriptome analysis, a series of plant development-related genes, including transcription factors, glutaredoxins, protein kinases, R-genes and microRNA target genes, were significantly altered. These results provide further evidence to support the important function of the C4 protein in begomovirus pathogenicity. These transgenic tomato plants could serve as basic genetic materials for further characterization of plant receptors that are interacting with the TYLCV C4.
Dynamic response and low voltage ride-through enhancement of brushless double-fed induction generator using Salp swarm optimization algorithm
Authors: Memon Ahsanullah , Wazir Bin Mustafa Mohd , Anjum Waqas , Ahmed Ahsan , Ullah Shafi , Altbawi Saleh Masoud Abdallah , Jumani Touqeer Ahmed , Khan Ilyas , Hamadneh Nawaf N.
Published: May 2022

A brushless double-fed induction generator (BDFIG) has shown tremendous success in wind turbines due to its robust brushless design, smooth operation, and variable speed characteristics. However, the research regarding controlling of machine during low voltage ride through (LVRT) need greater attention as it may cause total disconnection of machine. In addition, the BDFIG based wind turbines must be capable of providing controlled amount of reactive power to the grid as per modern grid code requirements. Also, a suitable dynamic response of machine during both normal and fault conditions needs to be ensured. This paper, as such, attempts to provide reactive power to the grid by analytically calculating the decaying flux and developing a rotor side converter control scheme accordingly. Furthermore, the dynamic response and LVRT capability of the BDFIG is enhanced by using one of the very intelligent optimization algorithms called the Salp Swarm Algorithm (SSA). To prove the efficacy of the proposed control scheme, its performance is compared with that of the particle swan optimization (PSO) based controller in terms of limiting the fault current, regulating active and reactive power, and maintaining the stable operation of the power system under identical operating conditions. The simulation results show that the proposed control scheme significantly improves the dynamic response and LVRT capability of the developed BDFIG based wind energy conversion system; thus proves its essence and efficacy.
Tropical wetlands and land use changes: The case of oil palm in neotropical riverine floodplains
Authors: Camacho-Valdez Vera , Rodiles-Hernández Rocío , Navarrete-Gutiérrez Darío A. , Valencia-Barrera Emmanuel
Published: May 2022

Oil palm plantations are expanding in Latin America due to the global demand for food and biofuels, and much of this expansion has occurred at expense of important tropical ecosystems. Nevertheless, there is limited knowledge about effects on aquatic ecosystems near to oil palm-dominated landscapes. In this study, we used Landsat 7 ETM+, Landsat 8 OLI imagery and high-resolution images in Google Earth to map the current extent of oil palm plantations and determined prior land use land cover (LULC) in the Usumacinta River Basin as a case-study site. In addition, we assess the proximity of the crop with aquatic ecosystems distributed in the Usumacinta floodplains and their potential effects. Based on our findings, the most significant change was characterized by the expansion of oil palm crop areas mainly at expenses of regional rainforest and previously intervened lands (e.g. secondary vegetation and agriculture). Although aquatic ecosystem class (e.g. rivers, lagoons and channels) decreased in surface around 3% during the study period (2001–2017), the change was not due to the expansion of oil palm lands. However, we find that more than 50% of oil palm cultivations are near (between 500 and 3000 m) to aquatic ecosystems and this could have significant environmental impacts on sediment and water quality. Oil palm crops tend to spatially concentrate in the Upper Usumacinta ecoregion (Guatemala), which is recognized as an area of important fish endemism. We argue that the basic information generated in this study is essential to have better land use decision-making in a region that is relative newcomer to oil palm boom.
Features of fibrosis regression abound in “non-cirrhotic” patients with resected hepatocellular carcinoma
Authors: Orr Christine E. , Wang Peter L. , Chen Lina , Wang Tao
Published: May 2022

Cirrhosis is a major risk factor for developing hepatocellular carcinoma (HCC). However, many surgically resected HCCs are presumably non-cirrhotic. The dynamic nature of chronic liver disease leads to periods of hepatic repair and fibrosis regression. We hypothesize that most resected HCCs, including those from non-cirrhotic patients, exhibit features of fibrosis regression in their background liver, suggesting previously more advanced liver disease. We reviewed the histology of 37 HCC resections performed between 2005–2020, including 30 from non-cirrhotic patients. The non-neoplastic liver was evaluated for features of liver disease and of the hepatic repair complex (HRC). CD34 immunohistochemistry was performed as a marker of sinusoidal capillarization. CD34 staining was evaluated manually and also by a digital image classifier algorithm. Overall, 28 cases (76%) had a high number of fibrosis regression and hepatic repair features (≥4 out of 8 features). Amongst the 30 non-cirrhotic patients, 21 (70%) showed a high number of repair features. Relative CD34 expression was increased in cases with a high number (≥4) of HRC features versus a low number (≤3) of features (p = 0.019). High HRC cases were more likely to exhibit nodular circumferential CD34 staining (p = 0.019). Our findings suggest that most resected HCC from non-cirrhotic patients display features of fibrosis regression in their background liver. Thus many, if not most, HCC patients who are “non-cirrhotic” may in fact have regressed cirrhosis. This finding reinforces that patients with regressed cirrhosis continue to be at high risk for HCC.
Efficacy versus abundancy: Comparing vaccination schemes
Authors: El Deeb Omar , Jalloul Maya
Published: May 2022

We introduce a novel compartmental model accounting for the effects of vaccine efficacy, deployment rates and timing of initiation of deployment. We simulate different scenarios and initial conditions, and we find that higher abundancy and rate of deployment of low efficacy vaccines lowers the cumulative number of deaths in comparison to slower deployment of high efficacy vaccines. We also forecast that, at the same daily deployment rate, the earlier introduction of vaccination schemes with lower efficacy would also lower the number of deaths with respect to a delayed introduction of high efficacy vaccines, which can however, still achieve lower numbers of infections and better herd immunity.
Establishing a framework towards monitoring HCV microelimination among men who have sex with men living with HIV in Germany: A modeling analysis
Authors: Marquez Lara K. , Ingiliz Patrick , Boesecke Christoph , Krznaric Ivanka , Schewe Knud , Lutz Thomas , Mauss Stefan , Christensen Stefan , Rockstroh Jürgen K. , Jain Sonia , He Feng , Wertheim Joel O. , Martin Natasha K.
Published: May 2022

Background

Prior to direct-acting antivirals (DAAs), HCV incidence rose among men who have sex with men (MSM) living with HIV infection in Germany despite high hepatitis C virus (HCV) treatment rates. We establish a HCV elimination modeling framework to evaluate whether existing treatment rates can achieve the World Health Organization (WHO) incidence target among MSM living with HIV in Germany.

Methods

To evaluate progress towards HCV elimination in Germany, we adapted a previously published HCV transmission model among MSM living with diagnosed HIV. We modelled HCV incidence and prevalence until 2030 (relative to 2015) under existing treatment and DAA scale-up and explored potential impacts of disruptions in treatment and behavioral risk reduction due to the COVID-19 pandemic.

Results

Continuing current treatment rates will result in stable HCV incidence among MSM living with HIV in Germany between 2015–2030. The WHO HCV incidence target is achievable under DAA scale-up to 100% treatment combined with treatment of those previously diagnosed and untreated (at a rate of 15%/year) and would result in greater reductions with early treatment (3 vs 6 months) reducing incidence from 4.0/100person-years to 0.8/100person-years by 2030. A 12-month disruption to HCV treatment (20% reduction) and risk behaviors (25%,50%,75% reduction) during the COVID-19 pandemic would result in a 15% relative increase in total HCV incidence in 2030 compared to that expected under the status quo.

Conclusions

HCV elimination among MSM living with HIV in Germany requires further DAA scale-up among those newly diagnosed combined with efforts to treat those previously diagnosed but untreated. Prospective monitoring will establish whether Germany is on track for HCV microelimination.
Built environment impact on the per capita cycling frequency of family——Based on two-level hierarchical linear model
Authors: Zhang Xiaonan , Wang Jianjun , Xue Jianfeng , Long Xueqin , Li Weijia , Lu Xiaojuan , Wang Sai
Published: May 2022

At present, there is less attention paid to the relationship between the frequency of travel and built environment, especially in households. In this paper, some of the determining factors in the frequency of daily cycling per household were explored based on the data from 2018 Daily Trip Survey in Xianyang, China. Then a two-level linear model was construct to identify the determining factors in the frequency of per capita daily cycling of household. According to the research results, 22.8% of the differences in the per capita cycling frequency of household are due to the differences between communities. In terms of community factors, the densities of road networks and educational facilities delivered a significantly positive impact on the per capita daily cycling frequency of family; on the contrary, the densities of medical facilities, intersections and POI delivered a significantly negative impact. Per capita cycling frequency varies considerably between households. For instance, the number of bicycles owned and the number of school-age children have a significantly positive impact on the per capita daily cycling frequency of family. However, car ownership, household income and occupation composition impose a significantly negative impact. The findings of this study would benefit the transportation engineers and planners who are keen to boost the use of active means of transportation for residents.
Effectiveness of a home telemonitoring program for patients with chronic obstructive pulmonary disease in Germany: Evidence from the first three years
Authors: Hofer Florian , Schreyögg Jonas , Stargardt Tom
Published: May 2022

Introduction

Chronic obstructive pulmonary disease (COPD) affects more than 6 million people in Germany. Monitoring the vital parameters of COPD patients remotely through telemonitoring may help doctors and patients prevent and treat acute exacerbations of COPD, improving patients’ quality of life and saving costs for the statutory health insurance system.

Objective

To evaluate the effects from October 2012 until December 2015 of a structured home telemonitoring program implemented by a statutory health insurer in Germany.

Methods

We conducted a retrospective cohort study using administrative data. After building a balanced control group using Entropy Balancing, we calculated difference-in-difference estimators to account for time-invariant heterogeneity. We estimated differences in mortality rates using Cox regression and conducted subgroup and sensitivity analyses to check the robustness of the base case results. We observed each patient in the program for up to 3 years depending on his or her time of enrolment.

Results

Among patients in the telemonitoring cohort, we observed significantly higher inpatient costs due to COPD (€524.2, p<0,05; €434.6, p<0.05) and outpatient costs (102.5, p<0.01; 78.8 p<0.05) during the first two years of the program. Additional cost categories were significantly increased during the first year of telemonitoring. We also observed a significantly higher number of drug prescriptions during all three years of the observation period (2.0500, p < 0.05; 0.7260, p < 0.05; 3.3170, p < 0.01) and a higher number of outpatient contacts during the first two years (0.945, p<0.01, 0.683, p<0.05). Furthermore, we found significantly improved survival rates for participants in the telemonitoring program (HR 0.68, p<0.001).

Conclusion

On one hand, telemonitoring was associated with higher health care expenditures, especially in the first year of the program. For example, we were able to identify a statistically significant increase in inpatient costs due to COPD, outpatient contacts and drug prescriptions among individuals participating in the telemonitoring program. On the other hand, the telemonitoring program was accompanied by a survival benefit, which might be related to higher adherence rates, more intense treatment, or an improved understanding of COPD among these patients.
Response rates and minimal residual disease outcomes as potential surrogates for progression-free survival in newly diagnosed multiple myeloma
Authors: Daniele Patrick , Mamolo Carla , Cappelleri Joseph C. , Bell Timothy , Neuhof Alexander , Tremblay Gabriel , Musat Mihaela , Forsythe Anna
Published: May 2022

Progression-free survival (PFS) is a common primary endpoint in newly diagnosed multiple myeloma (NDMM). Patients with NDMM typically have longer PFS and are more likely to achieve minimal residual disease (MRD) or complete response (CR) compared to patients with relapsed or refractory multiple myeloma. Response-based surrogate endpoints may hold value given the longer follow-up time required to evaluate PFS in NDMM. In this work, systematic literature reviews of Medline, Embase, and Cochrane databases (2010-06/2020) and relevant congresses (2018–2020) were performed to identify randomized clinical trials (RCTs) and real-world studies in NDMM reporting median PFS and objective response. Associations between PFS and each response endpoint were evaluated using Pearson’s product-moment correlation weighted by sample size in each RCT arm. Unadjusted and adjusted weighted linear regression models were applied to estimate the gain in median PFS associated with each response endpoint. Statistically significant correlations were identified for median PFS with overall response rate (ORR; Pearson r = 0.59), CR (r = 0.48), stringent CR (sCR; r = 0.68), and MRD (r = 0.69). The unadjusted models estimated 0.50 (95% CI: 0.36, 0.64; p<0.001), 0.42 (95% CI: 0.25, 0.58; p<0.001), 1.05 (95% CI: 0.58, 1.52; p<0.001), and 0.35 (95% CI: 0.12, 0.58; p = 0.006) months of median PFS gained per point of ORR, CR, sCR, and MRD, respectively. Associations for median PFS remained statistically significant in models adjusted for age and treatment type with ORR (0.35, 95% CI: 0.21, 0.49; p<0.001), and adjusted for age and International Staging System risk stage with CR (0.29, 95% CI: 0.16, 0.41; p<0.001). Due to small sample size, adjusted models could not be constructed for sCR or MRD. Nevertheless, evidence of significant survival benefit (p<0.05) associated with MRD negativity and sCR was identified across real-world studies. These findings provide support for the use of response outcomes as surrogate endpoints to estimate PFS benefit in NDMM.
Development and initial validation of the Japanese healthy work environment assessment tool for critical care settings
Authors: Kitayama Mio , Unoki Takeshi , Matsuda Yui , Matsuishi Yujiro , Kawai Yusuke , Iida Yasuo , Teramoto Mio , Tatsuno Junko , Hamamoto Miya
Published: May 2022

Aim

This study aims to translate the Healthy Work Environment Assessment Tool (HWE-AT) into Japanese and evaluate its validity and reliability.

Design and methods

The authors followed the guidelines for scale translation, adaptation, and validation in cross-cultural healthcare research. After translation and back-translation, a series of pilot studies were conducted to assess comprehensibility. Subsequently, an expert panel established the content validity. Content validity was calculated using the content validity index (CVI). Finally, we verified the construct validity and calculated the test-retest reliability.

Results

The updated HWE-AT achieved sufficient comprehensibility after conducting the two pilot tests. Content validity was calculated using the scale-level CVI/average and all the items were 1.00. The content validity indices CFI and RMSEA were 0.918 and 0.082, respectively. Intraclass correlation coefficients for all dimensions ranged from 0.618 to 0.903, indicating acceptable test-retest reliability. Our findings suggest that the Japanese version of the HWE-AT has good validity and reliability.
Prognostic value of upper respiratory tract microbes in children presenting to primary care with respiratory infections: A prospective cohort study
Authors: McGeoch Luke J. , Thornton Hannah V. , Blair Peter S. , Christensen Hannah , Turner Nicholas L. , Muir Peter , Vipond Barry , Redmond Niamh M. , Turnbull Sophie , Hay Alastair D.
Published: May 2022

Background

The association between upper respiratory tract microbial positivity and illness prognosis in children is unclear. This impedes clinical decision-making and means the utility of upper respiratory tract microbial point-of-care tests remains unknown. We investigated for relationships between pharyngeal microbes and symptom severity in children with suspected respiratory tract infection (RTI).

Methods

Baseline characteristics and pharyngeal swabs were collected from 2,296 children presenting to 58 general practices in Bristol, UK with acute cough and suspected RTI between 2011–2013. Post-consultation, parents recorded the severity of six RTI symptoms on a 0–6 scale daily for ≤28 days. We used multivariable hurdle regression, adjusting for clinical characteristics, antibiotics and other microbes, to investigate associations between respiratory microbes and mean symptom severity on days 2–4 post-presentation.

Results

Overall, 1,317 (57%) children with complete baseline, microbiological and symptom data were included. Baseline characteristics were similar in included participants and those lacking microbiological data. At least one virus was detected in 869 (66%) children, and at least one bacterium in 783 (60%). Compared to children with no virus detected (mean symptom severity score 1.52), adjusted mean symptom severity was 0.26 points higher in those testing positive for at least one virus (95% CI 0.15 to 0.38, p<0.001); and was also higher in those with detected Influenza B (0.44, 0.15 to 0.72, p = 0.003); RSV (0.41, 0.20 to 0.60, p<0.001); and Influenza A (0.25, -0.01 to 0.51, p = 0.059). Children positive for Enterovirus had a lower adjusted mean symptom severity (-0.24, -0.43 to -0.05, p = 0.013). Children with detected Bordetella pertussis (0.40, 0.00 to 0.79, p = 0.049) and those with detected Moraxella catarrhalis (-0.76, -1.06 to -0.45, p<0.001) respectively had higher and lower mean symptom severity compared to children without these bacteria.

Conclusions

There is a potential role for upper respiratory tract microbiological point-of-care tests in determining the prognosis of childhood RTIs.
Regretful pleasure: Toward an understanding of flow cost in media use
Authors: Pham Giang V. , Duff Brittany R. L.
Published: May 2022

Flow (state of intense focus) during media use has been largely considered a desirable experience, with technologies developed to maximize the chance of encountering flow in computer-mediated environments. However, the total absorption of attention due to flow could be problematic in contexts where the user has multiple predetermined goals, and engaging with the flow-inducing media could cost them resources that may be otherwise devoted to other goals. When flow imposes a cost on the user’s goal performance, it may also reduce their post-experience gratification with the flow-inducing media. The present study proposes a novel theoretical framework to begin understanding the potential cost of flow in media use with supporting evidence from two survey and vignette studies (N= 235 andN= 245). Its findings will extend human-computer interaction research by highlighting the double-edged impact that flow might have on media users’ larger goal performance and downstream well-being.
Survival of HT29 cancer cells is influenced by hepatocyte growth factor receptor inhibition through modulation of self-DNA-triggered TLR9-dependent autophagy response
Authors: Bohusné Barta Bettina , Simon Ágnes , Nagy Lőrinc , Dankó Titanilla , Raffay Regina Eszter , Petővári Gábor , Zsiros Viktória , Sebestyén Anna , Sipos Ferenc , Műzes Györgyi
Published: May 2022

HGFR activation drives the malignant progression of colorectal cancer, and its inhibition displays anti-autophagic activity. The interrelated role of HGFR inhibition and TLR9/autophagy signaling in HT29 cancer cells subjected to modified self-DNA treatments has not been clarified. We analyzed this complex interplay with cell metabolism and proliferation measurements, TLR9, HGFR and autophagy inhibitory assays and WES Simple Western blot-based autophagy flux measurements, gene expression analyses, immunocytochemistry, and transmission electron microscopy. The overexpression of MyD88 and caspase-3 was associated with enhanced HT29 cell proliferation, suggesting that incubation with self-DNAs could suppress the apoptosis-induced compensatory cell proliferation. HGFR inhibition blocked the proliferation-reducing effect of genomic and hypermethylated, but not that of fragmented DNA. Lowest cell proliferation was achieved with the concomitant use of genomic DNA, HGFR inhibitor, and chloroquine, when the proliferation stimulating effect of STAT3 overexpression could be outweighed by the inhibitory effect of LC3B, indicating the putative involvement of HGFR-mTOR-ULK1 molecular cascade in HGFR inhibitor-mediated autophagy. The most intense cell proliferation was caused by the co-administration of hypermethylated DNA, TLR9 and HGFR inhibitors, when decreased expression of both canonical and non-canonical HGFR signaling pathways and autophagy-related genes was present. The observed ultrastructural changes also support the context-dependent role of HGFR inhibition and autophagy on cell survival and proliferation. Further investigation of the influence of the studied signaling pathways and cellular processes can provide a basis for novel, individualized anti-cancer therapies.
A qualitative exploration of mental health services provided in community pharmacies
Authors: Crespo-Gonzalez Carmen , Dineen-Griffin Sarah , Rae John , Hill Rodney A.
Published: May 2022

The burden of mental health problems continues to grow worldwide. Community pharmacists’, as part of the primary care team, optimise care for people living with mental illness. This study aims to examine the factors that support or hinder the delivery of mental health services delivered in Australian community pharmacies and proposes ideas for improvement. A qualitative study was conducted comprising focus groups with community pharmacists and pharmacy staff across metropolitan, regional, and rural areas of New South Wales, Australia. Data were collected in eight focus groups between December 2020 and June 2021. Qualitative data were analysed using thematic analysis. Thirty-three community pharmacists and pharmacy staff participated in an initial round of focus groups. Eleven community pharmacists and pharmacy staff participated in a second round of focus groups. Twenty-four factors that enable or hinder the delivery of mental health services in community pharmacy were identified. Participant’s perception of a lack of recognition and integration of community pharmacy within primary care were identified as major barriers, in addition to consumers’ stigma and lack of awareness regarding service offering. Suggestions for improvement to mental health care delivery in community pharmacy included standardised practice through the use of protocols, remuneration and public awareness. A framework detailing the factors moderating pharmacists, pharmacy staff and consumers’ empowerment in mental health care delivery in community pharmacy is proposed. This study has highlighted that policy and funding support for mental health services is needed that complement and expand integrated models, promote access to services led by or are conducted in collaboration with pharmacists and recognise the professional contribution and competencies of community pharmacists in mental health care. The framework proposed may be a step to strengthening mental health support delivered in community pharmacies.
Disability, physical activity, and health-related quality of life in Australian adults: An investigation using 19 waves of a longitudinal cohort
Authors: Keramat Syed Afroz , Ahammed Benojir , Mohammed Aliu , Seidu Abdul-Aziz , Farjana Fariha , Hashmi Rubayyat , Ahmad Kabir , Haque Rezwanul , Ahmed Sazia , Ali Mohammad Afshar , Ahinkorah Bright Opoku
Published: May 2022

Background

Any form of long-term physical or mental impairment might negatively influence health-related quality of life (HRQoL). HRQoL, as an independent concept, covers a wide range of characteristics that includes physical, mental, social, and spiritual functions. People with disabilities are continuously exposed to multiple barriers that deteriorate their HRQoL. It also creates impairment in performing physical activities. However, experts opine regular physical exercise as an intervention to help disabled people. This research aims to investigate the association between disability and physical activity with HRQoL among the adult population in Australia.

Design

A retrospective cohort study.

Methods

This study utilized the most recent 19 waves of data (2002–2020) from the nationally representative Household, Income and Labour Dynamics in Australia (HILDA) survey. Component summary scores such as physical component summary (PCS) and mental component summary (MCS), and SF-6D utility scores were utilized to measure HRQoL. Random-effects GLS regression technique was fitted to estimate the association between disability and physical activity with HRQoL, after adjusting for a range of socio-demographic and health-related characteristics.

Results

Disability was negatively associated with the PCS (-5.95), MCS (-2.70) and SF-6D (-0.060) compared with non-disabled counterparts. However, respondents engaged in the recommended level of physical activity had substantial gain in PCS (b = 0.96), MCS (1.57), and SF-6D (0.021) scores. Besides, the results showed that performing the recommended level of physical activity in the presence of disability has lessen the negative effect of disability/ positive moderating effect of physical activity on PCS, MCS, and SF-6D scores by 1.84 points, 0.82 points, and 0.013 percentage points, respectively.

Conclusion

This study found an inverse association between disability and HRQoL among Australian adults. However, physical activity was associated with improved HRQoL. Therefore, public health interventions, such as the orientation of physical activities, have a higher potential to dwindle the burden regarding HRQoL.
The monoterpene 1,8-cineole prevents cerebral edema in a murine model of severe malaria
Authors: Santos Edgleyson C. dos , Silva Leandro S. , Pinheiro Alessandro S. , Teixeira Douglas E. , Peruchetti Diogo B. , Silva-Aguiar Rodrigo P. , Wendt Camila H. C. , Miranda Kildare R. , Coelho-de-Souza Andrelina N. , Leal-Cardoso José Henrique , Caruso-Neves Celso , Pinheiro Ana Acacia S.
Published: May 2022

1,8-Cineole is a naturally occurring compound found in essential oils of different plants and has well-known anti-inflammatory and antimicrobial activities. In the present work, we aimed to investigate its potential antimalarial effect, using the following experimental models: (1) the erythrocytic cycle ofPlasmodium falciparum; (2) an adhesion assay using brain microvascular endothelial cells; and (3) an experimental cerebral malaria animal model induced byPlasmodium bergheiANKA infection in susceptible mice. Using the erythrocytic cycle ofPlasmodium falciparum, we characterized the schizonticidal effect of 1,8-cineole. This compound decreased parasitemia in a dose-dependent manner with a half maximal inhibitory concentration of 1045.53 ± 63.30 μM. The inhibitory effect of 972 μM 1,8-cineole was irreversible and independent of parasitemia. Moreover, 1,8-cineole reduced the progression of intracellular development of the parasite over 2 cycles, inducing important morphological changes. Ultrastructure analysis revealed a massive loss of integrity of endomembranes and hemozoin crystals in infected erythrocytes treated with 1,8-cineole. The monoterpene reduced the adhesion index of infected erythrocytes to brain microvascular endothelial cells by 60%. Using the experimental cerebral malaria model, treatment of infected mice for 6 consecutive days with 100 mg/kg/day 1,8-cineole reduced cerebral edema with a 50% reduction in parasitemia. Our data suggest a potential antimalarial effect of 1,8-cineole with an impact on the parasite erythrocytic cycle and severe disease.
Hypoglycaemia due to insulin therapy for the management of hyperkalaemia in hospitalised adults: A scoping review
Authors: Chothia Mogamat-Yazied , Humphrey Toby , Schoonees Anel , Chikte Usuf Mohamed Ebrahim , Davids Mogamat Razeen
Published: May 2022

Introduction

Hyperkalaemia is a very common electrolyte disorder encountered in hospitalised patients. Although hypoglycaemia is a frequent complication of insulin therapy, it is often under-appreciated. We conducted a scoping review of this important complication, and of other adverse effects, of the treatment of hyperkalaemia in hospitalised adults to map existing research on this topic and to identify any knowledge gaps.

Materials and methods

We followed the PRISMA-ScR guidelines. Studies were eligible for inclusion if they reported on any adverse effects in hospitalised patients ≥18-years-old, with hyperkalaemia receiving treatment that included insulin. All eligible research from 1980 to 12 October 2021 were included. We searched Medline (PubMed), Embase (Ovid), the Cochrane Library, CINHAL, Africa-Wide Information, Web of Science Core Collection, LILACS and Epistemonikos. The protocol was prospectively registered with the Open Science Framework (https://osf.io/x8cs9).

Results

Sixty-two articles were included. The prevalence of hypoglycaemia by any definition was 17.2% (95% CI 16.6–17.8%). The median timing of hypoglycaemia was 124 minutes after insulin administration (IQR 102–168 minutes). There were no differences in the prevalence of hypoglycaemia when comparing insulin dose (<10 units vs. ≥10 units), rate of insulin administration (continuous vs. bolus), type of insulin (regular vs. short-acting) or timing of insulin administration relative to dextrose. However, lower insulin doses were associated with a reduced prevalence of severe hypoglycaemia (3.5% vs. 5.9%, P = 0.02). There was no difference regarding prevalence of hypoglycaemia by dextrose dose (≤25 g vs. >25 g); however, prevalence was lower when dextrose was administered as a continuous infusion compared with bolus administration (3.3% vs. 19.5%, P = 0.02). The most common predictor of hypoglycaemia was the pre-treatment serum glucose concentration (n = 13 studies), which ranged from < 5.6–7.8 mmol/L.

Conclusion

This is the first comprehensive review of the adverse effects following insulin therapy for hyperkalaemia. Hypoglycaemia remains a common adverse effect in hospitalised adults. Future randomised trials should focus on identifying the optimal regimen of insulin therapy to mitigate the risk of hypoglycaemia.