bluuebunny/crossref_metadata_2025 huggingface dataset:
Quality Assurance for Hepatitis C Virus Point-of-Care Diagnostics in Sub-Saharan Africa
Authors: Duah Evans , Mathebula Evans Mantiri , Mashamba-Thompson Tivani
Published: February 2023

As part of a multinational study to evaluate the Bioline Hepatitis C virus (HCV) point-of-care (POC) testing in sub-Saharan Africa (SSA), this narrative review summarises regulatory standards and quality indicators for validating and approving HCV clinical diagnostics. In addition, this review also provides a summary of their diagnostic evaluations using the REASSURED criteria as the benchmark and its implications on the WHO HCV elimination goals 2030.
Using A-Mode Ultrasound to Assess the Body Composition of Soccer Players: A Comparative Study of Prediction Formulas
Authors: Muntean Paul , Neagu Monica , Amaricai Elena , Haragus Horia G. , Onofrei Roxana Ramona , Neagu Adrian
Published: February 2023

For elite athletes, monitoring body composition is important for maximizing performance without health risks. Amplitude (A)-mode ultrasound (AUS) has attracted increasing attention as an alternative to skinfold thickness measurements commonly used for assessing the amount of body fat in athletes. AUS accuracy and precision, however, depend on the formula used to predict body fat percentage (%BF) from subcutaneous fat layer thicknesses. Therefore, this study evaluates the accuracy of the 1-point biceps (B1), 9-sites Parrillo, 3-sites Jackson and Pollock (JP3), and 7-sites Jackson and Pollock (JP7) formulas. Relying on the previous validation of the JP3 formula in college-aged male athletes, we took AUS measurements in 54 professional soccer players (aged 22.9 ± 3.83 y, mean ± SD) and compared the results given by different formulas. The Kruskal–Wallis test indicated significant differences (p < 10−6), and Conover’s post hoc test revealed that the JP3 and JP7 data come from the same distribution, whereas the data given by B1 and P9 differ from all the others. Lin’s concordance correlation coefficients for B1 vs. JP7, P9 vs. JP7, and JP3 vs. JP7 were 0.464, 0.341, and 0.909, respectively. The Bland–Altman analysis indicated mean differences of −0.5 %BF between JP3 and JP7, 4.7 %BF between P9 and JP7, and 3.1 %BF between B1 and JP7. This study suggests that JP7 and JP3 are equally valid, whereas P9 and B1 overestimate %BF in athletes.
Blood Biomarkers in Patients with Parkinson’s Disease: A Review in Context of Anesthetic Care
Authors: Joo Jin , Jeong Jongmin , Park Hue Jung
Published: February 2023

Parkinson’s disease (PD) is the second most common inflammatory neurodegenerative disorder after dementia. Preclinical and epidemiological data strongly suggest that chronic neuroinflammation slowly induces neuronal dysfunction. Activated microglia secrete several neurotoxic substances, such as chemokines and proinflammatory cytokines, which may promote blood–brain barrier (BBB) permeabilization. CD4+ T cells comprise proinflammatory cells such as T helper (Th) 1 and Th17 cells, as well as anti-inflammatory cells such as Th2 and T regulatory cells (Tregs). Th1 and Th17 cells can be detrimental to dopamine neurons, whereas Th2 and Tregs are neuroprotective. The results of studies on the serum levels of cytokines such as IFN-γ and TNF-α secreted by Th1 T cells, IL-8 and IL-10 secreted by Th2 T cells, and IL-17 secreted by Th17 cells in PD patients are not uniform. In addition, the relationships between serum cytokine levels and motor and non-motor symptoms of PD are controversial. Surgical stress and anesthesia induce inflammatory responses by disturbing the balance between pro- and anti-inflammatory cytokines, which may exacerbate the neuroinflammatory response in PD patients. Here we review studies on blood inflammatory biomarkers in PD patients and discuss the roles of surgery and anesthesia in PD progression.
Short Half-Life of Des-γ-Carboxy Prothrombin Is a Superior Factor for Early Prediction of Outcomes of Hepatocellular Carcinoma Treated with Radiofrequency Ablation
Authors: Yao Chih-Chien , Wang Jing-Houng , Chen Chien-Hung , Hung Chao-Hung , Yen Yi-Hao , Kee Kwong-Ming , Hu Tsung-Hui , Tsai Ming-Chao , Kuo Yuan-Hung , Lu Sheng-Nan
Published: February 2023

Background: The role of des-γ-carboxy prothrombin (DCP) in patients undergoing radiofrequency ablation (RFA) for hepatocellular carcinoma (HCC) needs to be clarified. Materials and methods: 174 HCC patients that underwent RFA were enrolled. We calculated the HLs of DCP from the available values before and on first day after ablation and assessed the correlation between HLs of DCP and RFA efficacy. Results: Of 174 patients, 63 with pre-ablation DCP concentrations of ≥80 mAU/mL were analyzed. The ROC analysis showed the optimal cut-off value of HLs of DCP for predicting RFA response was 47.5 h. Therefore, we defined short HLs of DCP < 48 h as a predictor of favorable treatment response. Of 43 patients with a complete radiological response, 34 (79.1%) had short HLs of DCP. In 36 patients with short HLs of DCP, 34 (94.4%) had a complete radiologic response. The sensitivity, specificity, accuracy, positive predictive value, and negative predictive value were 79.1%, 90.0%, 82.5%, 94.4%, and 66.7%. During the 12-month follow-up, patients who had short HLs of DCP had a better disease-free survival rate than patients with long HLs of DCP (p < 0.001). Conclusions: Short HLs of DCP < 48 h calculated on the first day post-RFA are a useful predictor for treatment response and recurrence-free survival after RFA.
An Inexact Feasible Quantum Interior Point Method for Linearly Constrained Quadratic Optimization
Authors: Wu Zeguan , Mohammadisiahroudi Mohammadhossein , Augustino Brandon , Yang Xiu , Terlaky Tamás
Published: February 2023

Quantum linear system algorithms (QLSAs) have the potential to speed up algorithms that rely on solving linear systems. Interior point methods (IPMs) yield a fundamental family of polynomial-time algorithms for solving optimization problems. IPMs solve a Newton linear system at each iteration to compute the search direction; thus, QLSAs can potentially speed up IPMs. Due to the noise in contemporary quantum computers, quantum-assisted IPMs (QIPMs) only admit an inexact solution to the Newton linear system. Typically, an inexact search direction leads to an infeasible solution, so, to overcome this, we propose an inexact-feasible QIPM (IF-QIPM) for solving linearly constrained quadratic optimization problems. We also apply the algorithm to ℓ1-norm soft margin support vector machine (SVM) problems, and demonstrate that our algorithm enjoys a speedup in the dimension over existing approaches. This complexity bound is better than any existing classical or quantum algorithm that produces a classical solution.
Unconstrained Lagrangian Variational Principles for the Einstein Field Equations
Authors: Cremaschini Claudio , Tessarotto Massimo
Published: February 2023

This paper deals with the problem of establishing a systematic theoretical formulation of variational principles for the continuum gravitational field dynamics of classical General Relativity (GR). In this reference, the existence of multiple Lagrangian functions underlying the Einstein field equations (EFE) but having different physical connotations is pointed out. Given validity of the Principle of Manifest Covariance (PMC), a set of corresponding variational principles can be constructed. These are classified in two categories, respectively, referred to as constrained and unconstrained Lagrangian principles. They differ for the normalization properties required to be satisfied by the variational fields with respect to the analogous conditions holding for the extremal fields. However, it is proved that only the unconstrained framework correctly reproduces EFE as extremal equations. Remarkably, the synchronous variational principle recently discovered belongs to this category. Instead, the constrained class can reproduce the Hilbert–Einstein formulation, although its validity demands unavoidably violation of PMC. In view of the mathematical structure of GR based on tensor representation and its conceptual meaning, it is therefore concluded that the unconstrained variational setting should be regarded as the natural and more fundamental framework for the establishment of the variational theory of EFE and the consequent formulation of consistent Hamiltonian and quantum gravity theories.
Thermodynamics of Quantum Spin-Bath Depolarization
Authors: Dasari Durga Bhaktavatsala Rao
Published: February 2023

We analyze here through exact calculations the thermodynamical effects in depolarizing a quantum spin-bath initially at zero temperature through a quantum probe coupled to an infinite temperature bath by evaluating the heat and entropy changes. We show that the correlations induced in the bath during the depolarizing process does not allow for the entropy of the bath to increase towards its maximal limit. On the contrary, the energy deposited in the bath can be completely extracted in a finite time. We explore these findings through an exactly solvable central spin model, wherein a central spin-1/2 system is homogeneously coupled to a bath of identical spins. Further, we show that, upon destroying these unwanted correlations, we boost the rate of both energy extraction and entropy towards their limiting values. We envisage that these studies are relevant for quantum battery research wherein both charging and discharging processes are key to characterizing the battery performance.
Working with Convex Responses: Antifragility from Finance to Oncology
Authors: Taleb Nassim Nicholas , West Jeffrey
Published: February 2023

We extend techniques and learnings about the stochastic properties of nonlinear responses from finance to medicine, particularly oncology, where it can inform dosing and intervention. We define antifragility. We propose uses of risk analysis for medical problems, through the properties of nonlinear responses (convex or concave). We (1) link the convexity/concavity of the dose-response function to the statistical properties of the results; (2) define “antifragility” as a mathematical property for local beneficial convex responses and the generalization of “fragility” as its opposite, locally concave in the tails of the statistical distribution; (3) propose mathematically tractable relations between dosage, severity of conditions, and iatrogenics. In short, we propose a framework to integrate the necessary consequences of nonlinearities in evidence-based oncology and more general clinical risk management.
Improvement of Piglet Rearing’s Energy Efficiency and Sustainability Using Air-to-Air Heat Exchangers—A Two-Year Case Study
Authors: Deeken Hauke F. , Lengling Alexandra , Krommweh Manuel S. , Büscher Wolfgang
Published: February 2023

Pig farming in mechanically ventilated barns requires much electricity for ventilation or exhaust air purification. Furthermore, thermal energy is needed to fulfill the animals’ temperature requirements, especially in piglet rearing. Electrical and thermal energy input leads to CO2 emissions and operating costs. Up to 90% of heat losses are due to the exhausted air. Heat exchangers can recover thermal energy from the warm exhaust air and transfer it to the cold fresh air. This study aimed to investigate energy consumption, efficiency, CO2 emissions, and energy costs when using heat exchangers in a German piglet rearing barn under practical conditions in combination with exhaust air purification. The following parameters were obtained for a two-year period: air temperatures, air flow rates, and electricity and liquefied natural gas consumption; the latter were used to calculate CO2 emissions and energy costs. In total, 576,042 kWhel,th and 616,893 kWhel,th (years 1 and 2) of energy were provided, including 290,414 kWhth and 317,913 kWhth of thermal energy recovered. Using heat exchangers reduced CO2 emissions by up to 37.5% and energy costs by up to 19.7% per year. The study shows that piglet rearing can increase its ecological and environmental sustainability by using heat recovery.
CO2 Compression and Dehydration for Transport and Geological Storage
Authors: Bielka Paweł , Kuczyński Szymon , Nagy Stanisław
Published: February 2023

Observation of the greenhouse effect prompts the consideration of every possibility of reducing anthropogenic carbon dioxide emissions. One of the key methods that has been the subject of much research is Carbon Dioxide Capture and Storage. The purpose of this study was to investigate the main technologies of CO2 capture, separation, and dehydration as well as methods of its transport and methodology of selecting a suitable geological storage site. An installation of dehydration and compression of carbon dioxide captured after the post-combustion was designed at a temperature of 35 °C, a pressure of 1.51 bar, and a mass flow rate of 2.449 million tons/year, assuming that the geological storage site is located at 30 km from the capture place. For the dehydration process, a multistage compression and cooling system were applied, combined with a triethylene glycol (TEG) dehydration unit. The mass flow rate of TEG was selected as 0.5 kg/s. H2O out of the TEG unit was 26.6 ppm. The amount of energy required to compress the gas was minimized by adopting a maximum post-compression gas temperature of 95 °C for each cycle, thereby reducing plant operating costs. The total power demand was 7047 kW, 15,990 kW, and 24,471 kW, and the total received heat input was 13,880.76 kW, 31,620.07 kW, and 47,035.66 kW for 25%, 60%, and 100% plant load, respectively. The use of more compressors reduces the gas temperature downstream through successive compression stages. It also decreases the total amount of energy required to power the entire plant and the amount of heat that must be collected during the gas stream cooling process. The integration of CO2 compression and cooling system to recover heat and increase the efficiency of power units should be considered.
Spontaneous Imbibition and Core Flooding Experiments of Enhanced Oil Recovery in Tight Reservoirs with Surfactants
Authors: Zhang Shaojie , Zhu Feng , Xu Jin , Liu Peng , Chen Shangbin , Wang Yang
Published: February 2023

Despite the implementation of hydraulic fracturing technologies, the oil recovery in tight oil reservoirs is still poor. In this study, cationic, anionic, and nonionic surfactants of various sorts were investigated to improve oil recovery in tight carbonate cores from the Middle Bakken Formation in the Williston Basin. Petrophysical investigations were performed on the samples prior to the imbibition and core-flooding experiments. The composition of the minerals was examined using the XRD technique. To investigate the pore-size distribution and microstructures, nitrogen adsorption and SEM techniques were applied. The next step involved brine and surfactant imbibition for six Bakken cores and two Berea sandstone cores. The core samples were completely saturated with Bakken crude oil prior to the experiments. The core plugs were then submerged into the brine and surfactant solutions. The volume of recovered oil was measured using imbibition cells as part of experiments involving brine and surfactant ingestion into oil-filled cores. According to the findings, oil recovery from brine imbibition ranges from 4.3% to 15%, whereas oil recovery from surfactant imbibition can range from 9% to 28%. According to the findings, core samples with more clay and larger pore diameters produce higher levels of oil recovery. Additionally, two tight Bakken core samples were used in core-flooding tests. Brine and a separate surfactant solution were the injected fluids. The primary oil recovery from brine flooding on core samples is between 23% and 25%, according to the results. The maximum oil recovery by second-stage surfactant flooding is approximately 33% and 35%. The anionic surfactants appear to yield a better oil recovery in tight Bakken rocks, possibly due to their higher carbonate mineral concentrations, especially clays, according to both the core-scale imbibition and flooding experiments. For studied samples with larger pore sizes, the oil recovery is higher. The knowledge of the impacts of mineral composition, pore size, and surfactant types on oil recovery in tight carbonate rocks is improved by this study.
A Comparison of Double-End Partial Discharge Localization Algorithms in Power Cables
Authors: Abu Bakar Asfarina , Yii Chai Chang , Fern Chin Kui , Hou Pin Yoong , Lago Herwansyah , Rohani Mohamad Nur Khairul Hafizi
Published: February 2023

The double-end partial discharge (PD) measurement method is the most common method for measuring and localizing PD sources in power cables. The sensitivity of the PD sensor, the processing speed of the data acquisition unit, and the method of the PD localization algorithm are the three main keys to ensuring the accuracy of the PD source localization on power cables. A new multi-end PD localization algorithm known as segmented correlation trimmed mean (SCTM) has recently demonstrated excellent accuracy in the localization of PD sources on power cables. The algorithm, however, is only applicable to multi-end PD measurement methods. In this paper, the mathematical equation of the SCTM algorithm is customized to match the double-end PD measurement method. A MATLAB simulation was conducted to assess the performance of the SCTM algorithm in the double-end PD measurement method. The maximum peak detection (MPD) algorithm, segmented correlation (SC), and SCTM algorithm were compared as PD localization algorithms. The SC algorithms have shown that identifying the correlation bond between two cues instead of the peak of the PD signal in the MPD algorithm significantly increases the PD localization accuracy. The results show that the SCTM algorithm outperforms the MPD and SC algorithms in terms of accuracy.
Analytical Approach to Current Rating of Three-Phase Power Cable with Round Conductors
Authors: Szczegielniak Tomasz , Jabłoński Paweł , Kusiak Dariusz
Published: February 2023

The continuous increase in the demand for electricity makes it necessary to modernize or build new transmission lines. This, in turn, results in research that is still being carried out on the optimal use of power cables. In the paper, an improved analytical method for the determination of the current rating of power cables was proposed. The method for determining the ampacity of the power cable presented in the IEC standard assumes that power losses in the phase conductors and screens are determined by taking into account skin and the proximity effects on the basis of tabulated coefficients. The methodology proposed in the paper is based on the method presented in the IEC standard, but the power losses in the conductive elements of the cable are determined analytically, which offers higher accuracy. In order to validate the analytical method proposed in this paper, numerical calculations based on the finite element method with very fine mesh were also performed. Exemplary calculations carried out for three types of cables with use of the proposed method, IEC standard and finite elements showed very good agreement in the results. The proposed method requires more computational effort, but it offers more accurate results than the IEC standard and can be used when higher accuracy is required. It can also serve as a reference point for simplified calculations.
Mobility-as-a-Service as a Catalyst for Urban Transport Integration in Conditions of Uncertainty
Authors: Chmiel Beata , Pawlowska Barbara , Szmelter-Jarosz Agnieszka
Published: February 2023

Sudden events are being observed more often (pandemics, armed conflicts, high inflation, etc.). Urban transport systems are especially susceptible to sudden disruptions as manifested by sharp changes in user preferences and demand for transport services. Mobility as a service (MaaS) aims to improve the efficiency of urban transport systems and support the integration of various forms of transport. The main goal of the research is to assess the level of MaaS use and check what characteristics could influence using MaaS by different groups of users. A case study of the Tri-City, the largest urban centre in northern Poland, was used to examine the possibility of implementing MaaS. We assumed that the use of IT applications would help to reduce the challenges faced by mobility in modern cities. We hypothesised that MaaS is conducive to integrating transport in the city. Knowledge of MaaS acceptance is essential in implementing this concept and can help local authorities respond to sudden disruptions by increasing flexibility in shaping urban mobility. Poland still lacks a universal multimodal solution that would help optimise travel within the cities and actions should be undertaken to popularise digital solutions that improve the efficiency and organisation of PT.
Effectiveness of Urban Mobility Decarbonization Instruments
Authors: Karoń Grzegorz , Tomanek Robert
Published: February 2023

Decarbonization of urban mobility requires complex, interconnected actions. The instruments for decarbonizing urban mobility include urban collective transport, bicycles and means of personal transport, cybermobility and electromobility. The article presents the results of research on the transport behavior of the creative class, the effects of the COVID-19 pandemic on the functioning of urban mobility, and the results of research on the efficiency of electromobility in the field of the implementation of battery-powered buses. The research was aimed at a critical assessment of the current mobility model and creation of the basis for change in this area. In particular, these studies focused on changes in transport needs and greater use of cyberspace for various activities, which should reduce the volume of physical flows of people in urban transport networks. The aim of the article is to present the conclusions of these studies in terms of their use to change mobility models in cities. The practical significance of the presented research consists in indicating the factors of building individualized urban mobility strategies, with particular emphasis on the issues of sustainable development and decarbonization. The authors’ contribution to the development of science is demonstration of the possibilities of changing the mobility model in the city and reducing greenhouse gas emissions. In particular, this applies to urban metropolises that are moving away from heavy mining and processing industries, i.e., the number of jobs in business and educational services is increasing, and the industry of new technologies is developing. The conclusions formulated in the paper indicate, in particular, the significant decarbonization potential of cybermobility and the use of zero-emission means of transport, such as bicycles and scooters, which can be released by changing the approach to the organization of work and learning, in particular their virtualization and deconcentration of travel sources.
Designing Coupling of 2-Dimensional PhotoRecepto-Conversion Scheme (2DPRCS) with Clean Unit System Platform (CUSP)
Authors: Ishibashi Akira , Liang Sheng-Fu , Kato Naoto , Zhou Ziling , Hsieh Tsung-Hao , Matsuda Junji , Sawamura Nobuo
Published: February 2023

There has been so far no energo-environmental system, whose design is considered energy-wise as well as cleanliness-wise, put in practical use despite the fact that those systems would be of huge potential importance as disaster shelters for casualties and/or infectious disease patients, in particular, those of COVID-19. We have designed the energo-environmental system based on the 2-Dimensional PhotoRecepto-Conversion Scheme (2DPRCS) and Clean Unit System Platform (CUSP) technologies. We have demonstrated the energo-environmental system can be as clean as US 209D class 1000 or better, quite handily, in a couple of minutes. As for the solar-cell-based energy generation part, we have shown that the needed electric power could be generated using our original technology of the 2DPRCS by simulations, as the possible first application of casualties’ and patients’ highly clean rest-space that has monitoring ability of the status of those people including sleep assessment. This energo-environmental clean system would be realized with the implementation of 2DPRCS in the near future.
Clinical Features of Transient Growth Hormone Deficiency
Authors: Sakai Yuki , Ikegawa Kento , Shimura Kazuhiro , Hasegawa Yukihiro
Published: February 2023

Background: Most patients with idiopathic growth hormone deficiency (iGHD) in childhood have normal GH stimulation test results in adulthood. The present study aimed to investigate the characteristics and possible etiology of transient iGHD. Methods: Patients with childhood-onset iGHD who completed their GH treatment between March 2010 and March 2021 were retrospectively studied. Patients with a clear history of child abuse or constitutional delay of growth and puberty were excluded. Ten patients with a diagnosis of iGHD based on a decreased growth rate and growth hormone stimulation test findings at the time of onset were included. Retesting demonstrated that these patients had a normal GH level. Results: Five patients had insufficient weight gain (BMI SD score < −1.0 at the start of treatment or a decrease in BMI SD score > 1.0 from one year before treatment to the start of treatment). The other five patients had no remarkable clinical features. One patient had decreased height velocity at the same time as their sibling. Conclusion: Insufficient pre-treatment weight gain or a familial cluster of cases may be related to low GH peaks of GHST, leading to a diagnosis of transient GHD.
Lower Sensitivity of Soil Carbon and Nitrogen to Regional Temperature Change in Karst Forests Than in Non-Karst Forests
Authors: Li Yunfan , Yang Rong , Hu Peilei , Xiao Dan , Wang Zhongcheng , Zhang Wei , Wang Kelin
Published: February 2023

Lithology has such an important effect on the sustainability of soil carbon (C) pools. Forests are an important part of terrestrial C sinks; yet, it is unclear whether their soil carbon sensitivity to temperature changes is regulated by lithology, especially in karst ecosystems, which are widely distributed globally. Along a climate gradient in the subtropical region of southwest China, we compared the contents of soil organic C (SOC) and total N (TN) in karst and non-karst forests. The data were analyzed and processed using ANOVA, regression analysis, and random forest. The results showed that the karst forests had significantly higher SOC and TN contents but lower ratio of SOC to TN (C:N) than non-karst forests, mainly because of the higher soil calcium (Ca) content and microbial biomass. With rising mean annual temperature (MAT), SOC and TN contents in non-karst forests significantly decreased, whereas in karst forests they were not correlated with MAT; while, the opposite was true for C:N. In karst forests, soil Ca constrain warming induced decomposition of SOC and TN by forming stable complexes with SOM through exchangeable Ca, and by promoting aggregate stability through the role of calcium carbonate. The correlation between δ13C and the logarithm of SOC concentration also supported that conclusion. In karst forests compared to non-karst forests, soil C pools are larger and less sensitive to regional temperature change. Nevertheless, climate warming may still accelerate soil C loss in karst forests by increasing microbial C limitation. Thus, soil C sequestration potential and loss risk coexist in karst areas. The ratio of SOC to TN (C:N) is regulated through appropriate management measures in the process of karst vegetation restoration, thus promoting long-term stable sequestration of soil carbon pools.
Linkage between Leaf–Litter–Soil, Microbial Resource Limitation, and Carbon-Use Efficiency in Successive Chinese Fir (Cunninghamia lanceolata) Plantations
Authors: Shen Lu , Ye Shaoming , Liu Haiyu , Deng Xiangsheng , He Peng , Cheng Fei
Published: February 2023

Chinese fir (Cunninghamia lanceolata) is a kind of evergreen coniferous tree species, the expansion of its pure forest area and multiple generations of continuous planting has led to a decline of stand quality and woodland fertility. To further investigate the relationship between leaf, litter, and soil stoichiometry, microbial community status, and microbial resource limitation of Chinese fir after continuous planting. We studied the C, N, and P stoichiometries of leaf, litter, and soil from successive rotations of Chinese fir plantations. In addition to this, soil microbial biomass C, N, and P, extracellular enzymes, as well as the soil microbial community composition, were determined. The continuous planting of Chinese fir significantly increased the leaf N and P contents and decreased the C content of litter, and the soil C:N and C:P ratios, thus leading to a soil stoichiometric imbalance. The continuous planting of Chinese fir plantations significantly increased the soil microbial biomass. Compared with the first-generation plantations, the N and P contents of the second- and third-generation plantations increased by 37.11%, 21.83% and 46.28%, 73.38%, respectively, thus alleviating the restriction of microbial N and P. Under continuous planting, the extracellular enzyme activities of N (NAG + LAP) and P (AP) were significantly decreased, and those of the second- and third-generation plantations were significantly decreased by 7.05%, 9.43% and 11.79%, 48.94%, respectively, compared with those of the first-generation plantations, resulting in an increase of 7.85 and 3.19% in carbon-use efficiency. The fungi:bacteria (F:B) ratio of the soil microbial community was elevated in successive plantations. The least squares pathway model (PLS-PM) indicated that the stoichiometric ratio of ecological enzymes had an indirect negative effect on CUE, and was the strongest predictor. This study showed that the successive plantation of Chinese fir resulted in a leaf, litter, and soil stoichiometric imbalance, further affecting community composition and resource limitation of soil microorganisms.
Infestation Phases and Impacts of Dryocoetes confusus in Subalpine Fir Forests of Southern British Columbia
Authors: Maclauchlan Lorraine E. , Stock Arthur J. , Brooks Julie E.
Published: February 2023

Subalpine fir mortality and stand decline are increasingly evident in British Columbia (B.C.). This long-term study confirmed Dryocoetes confusus to be the major disturbance agent in high-elevation subalpine forests, killing over two-thirds of subalpine fir in eleven one-hectare study plots. D. confusus infestations in mature stands can be described as early-, mid-, or late-phase. The transition from the early- through late-phase is characterized by a lowered stem density as high levels of D. confusus attack and remove the largest trees, while other mortality factors kill smaller trees. Initially, live subalpine fir density and D. confusus activity varied among plots. By a final assessment, very little difference was observed in live stems per hectare. Mortality from all factors ranged from 0.5% to 5% annually, reaching as high as 80% in-stand mortality with >6 times more dead than live volume. When subalpine fir density was reduced to <400 sph, the D. confusus attack rate declined. Cumulative mortality increased the average gap size in plots from 11 m2 to 18 m2. Our study also showed that D. confusus might be able to switch to a univoltine life cycle, taking advantage of warmer and longer growing seasons that, in part, could explain the rapid increase in mortality in stands.
The Characteristics of Gas and Particulate Emissions from Smouldering Combustion in the Pinus pumila Forest of Huzhong National Nature Reserve of the Daxing’an Mountains
Authors: Tang Shuyuan , Yin Sainan , Shan Yanlong , Yu Bo , Cui Chenxi , Cao Lili
Published: February 2023

Smouldering combustion can emit a large amount of CO2, CO and particulate matter (PM). Moisture content is an important factor of the emission characteristics. As the hot spot of forest smouldering combustion, the gas and particulate emissions of the Huzhong National Nature Reserve with different moisture contents are discussed herein. The emission factors (EF) of CO2 and CO were 100.71 ± 39.14 g/kg and 11.76 ± 3.89 g/kg, respectively. The EF of PM2.5, PM4 and PM10 were 87.11 ± 19.47 g/kg, 353.37 ±159.25 g/kg and 602.59 ± 276.80 g/kg, respectively. PM2.5 accounted for 16.59 ± 5.25% of the PM, and PM4 and PM10 were 54.03 ± 13.46% and 91.00 ± 10.81%, respectively. There was no significant difference in the EF of CO2 and CO with different moisture contents, nor in the EF of PM2.5, but there was a significant difference in the EF of PM4 and PM10 with different moisture contents. In addition, the peak of CO2 and CO appeared at 2~3 h; the peak of PM2.5 lagged behind that of PM4 and PM10. According to the regression analysis, experimental expressions were obtained for the modified combustion efficiency (MCE) and the EF of PM.
Establishment of a Highly Efficient In Vitro Propagation System of Diospyros lotus
Authors: Liu Yang , Lu Xiaoyu , Zhang Hui , Li Shuzhan , Li Ze
Published: February 2023

Persimmon (Diospyros) is an economically important tree widely cultivated for woody grain production in China, and Diospyros lotus is mainly used as the grafting stock of persimmon. However, the breeding of stress-resistant rootstocks of D. lotus using molecular means has yet to be achieved; in particular, an efficient blade-regeneration system has not been perfected to date. This study examined the effects of different plant-growth regulators and concentrations on the primary culture of stems with buds, the induction of leaf callus, the differentiation of adventitious shoots, and rooting culture of D. lotus. The optimal formula for inducing axillary buds from stems with buds was 1/2 Murashige and Skoog (MS) medium containing 2.0 mg/L 6-benzylaminopurine (6-BA) and 0.5 mg/L naphthaleneacetic acid (NAA), in which the induction rate of axillary buds approached 67.1%. The best medium formula for leaf callus induction was 1/2 MS medium containing 2.0 mg/L 6-BA and 0.5 mg/L NAA. Then callus was transferred to 1/2 MS medium containing 2.0 mg/L 6-(γ,γ-dimethylallylamino)purine (2iP), 2.0 mg/L thidizuron (TDZ), and 40 g/L sucrose to induce adventitious shoots after dark culture for 48 h, resulting in 7.9 shoots per explant and a 75.2% induction frequency of adventitious shoots. In addition, it was difficult to induce adventitious shoots from callus after six times of continuous transfer and differentiation. The adventitious shoots were transferred to 1/2 MS medium containing 2.0 mg/L zeatin (ZT) and 2.0 mg/L 2iP for proliferation culture, in which the multiplication coefficient approached 7.6. The adventitious shoots after multiplication were inoculated into 1/2 MS + 1.0 mg/L IBA + 0.5 mg/L NAA medium, the rooting rate was 70.2%, and the average number of heels was 9.6. Thus, studies in this area are expected to facilitate rapid and excellent growth, as well as theoretical support for factory saplings’ care and molecular breeding.
Bark Stripping by Deer Disturbs Regeneration in a Larix–Abies Subalpine Forest
Authors: Nagaike Takuo
Published: February 2023

Deer can slow the natural succession of forests from light-demanding to shade-tolerant species or even keep forests in an early successional stage. This study examined the effects of bark stripping by deer (Cervus nippon) on the stand dynamics in a Larix kaempferi–Abies veitchii subalpine forest in central Japan. Bark stripping by C. nippon was concentrated on smaller A. veitchii individuals, and the number of stems with stripped bark increased over the study period (2007–2019). Unlike other tree species in the study plot, the main mortality factor of A. veitchii was bark stripping. In addition, the mortality factors of A. veitchii clearly differed among tree sizes: smaller A. veitchii trees (diameter at breast height ≤ 15 cm) died owing mainly to bark stripping, whereas larger individuals died owing mainly to uprooting, snapping, and standing dead trees. At this forest site, bark-stripping deer preferred the late-successional species (A. veitchii) to the early-successional species (L. kaempferi), and consequently, the progress of succession was disturbed.
Assessing the Impact of Confirmation of Rights and Collective Trust on Farmer’s Forestry Management and Protection Behaviour—A Case of Collective Forest Areas in Zhejiang and Jiangxi Provinces, China
Authors: Li Lipeng , Ding Xiuling , Sarkar Apurbo , Li Hua
Published: February 2023

Confirmation of rights and collective trust (interpersonal and institutional) can act as primary factors for facilitating effective forest management and conservation. Collective forests are lands held collectively by either rural or indigenous communities based on a shared history, language, culture, or lineage. It is an institutional arrangement in which communities are involved wholly or partly in decision-making and contribute knowledge and labour to achieve healthy forests and social well-being. Based on the existing literature, the nature of collective forest rights and trust can be derived from social, rather than economic, prospects. Therefore, based on the institutional theory, this study constructs a theoretical framework to verify the impact of the confirmation of rights and trust on farmers’ forestry management conservation behaviour. The empirical setup of the study was comprised of a data set of 682 collective forest farmers in Zhejiang and Jiangxi provinces, China. We utilized the negative binomial regression model to quantify the proposed framework. The main conclusions were as follows. Confirmation of rights and collective trust had a significant positive role in promoting farmers’ forestry management and protection behaviour. Increasing the confirmation of rights and trusts by one unit increased the number of farmers’ forest management and protection by 1.846 and 2.631 times, respectively. The interaction between confirmation and trust did not substantially affect farmers’ behaviour. The diverse forest labour force, the total area of forest land, and the number of forest plots had a significant positive impact, while the head of the village and the number of migrant workers significantly and negatively impacted farmers’ behaviour. Therefore, it is necessary to further improve the forestry land rights system and strengthen the trust mechanism so that it can become an effective institutional incentive for farmers to manage and protect forests.
The Inhibitory Effects of RNA-Interference-Mediated Guanylate Cyclase Knockdown on Larval Metamorphosis and Early Progeny Growth of Razor Clam
Authors: Han Yuting , Li Beibei , Li Yifeng , Niu Donghong
Published: February 2023

Guanylate cyclase (GC, cGMPase) is a key enzyme in organisms, catalyzing the synthesis of cGMP from GTP, thus making cGMP work. cGMP plays a vital role in the regulation of cell and biological growth as a second messenger in signaling pathways. In this study, we screened and identified cGMPase from the razor clam Sinonovacula constricta, which encoded 1257 amino acids and was widely expressed in different tissues, especially the gill and liver. We also screened one double-stranded RNA (dsRNA), cGMPase, which was used to knockdown cGMPase at three larval metamorphosis development stages: trochophores-veliger larve, veliger larve-umbo larve, and umbo larve-creeping larvae. We showed that interference at these stages significantly inhibited larval metamorphosis and survival rates. cGMPase knockdown resulted in an average metamorphosis rate of 60% and an average mortality rate of 50% when compared with control clams. After 50 days, shell length and body weight were inhibited to 53% and 66%, respectively. Thus, cGMPase appeared to regulate metamorphosis development and growth in S. constricta. By examining the role of the key gene in the metamorphosis development of S. constricta larvae and the growth and development period, we can provide some data reference for studying the growth and development mechanism of shellfish, and the results provided basic information for the breeding of S. constricta.
DNA Barcoding of Wild Plants with Potential Medicinal Properties from Faifa Mountains in Saudi Arabia
Authors: Safhi Fatmah Ahmed , Alshamrani Salha Mesfer , Bogmaza Abdullah Farag Mohammed , El-Moneim Diaa Abd
Published: February 2023

Wild medicinal plants are the main source of active ingredients and provide a continuous natural source for many folk medicinal products, a role that is important for society’s health with an impressive record of utilization. Thus, surveying, conserving, and precisely identifying wild medicinal plants is required. The current study aimed to precisely identify fourteen wild-sourced medicinal plants from southwest Saudi Arabia, within the Fifa mountains area located in Jazan province, using the DNA barcoding technique. Two DNA regions (nuclear ITS and chloroplast rbcL) were sequenced and analyzed for the collected species using BLAST-based and phylogeny-based identification methods. Based on our analysis, ten of the fourteen species were successfully identified by DNA barcoding, five were identified as morphologically inspected, and three were morphologically indifferent. The study was able to distinguish some key medicinal species and highlight the importance of combining morphological observation with DNA barcoding to ensure the precise identification of wild plants, especially if they are medicinally relevant and associated with public health and safety usage.
Role of miR-143 and miR-146 in Risk Evaluation of Coronary Artery Diseases in Autopsied Samples
Authors: Tie Jian , Takanari Hiroki , Ota Koya , Okuda Takahisa
Published: February 2023

Coronary artery disease (CAD) is a common and fatal cardiovascular disease. Among known CAD risk factors, miRNA polymorphisms, such as Has-miR-143 (rs41291957 C>G) and Has-miR-146a (rs2910164 G>A), have emerged as important genetic markers of CAD. Despite many genetic association studies in multiple populations, no study assessing the association between CAD risk and SNPs of miR-143 and miR-146 was documented in the Japanese people. Therefore, using the TaqMan SNP assay, we investigated two SNP genotypes in 151 subjects with forensic autopsy-proven CAD. After pathological observation, we used ImageJ software to assess the degree of coronary artery atresia. Moreover, the genotypes and miRNA content of the two groups of samples with atresia <10% and >10% were analyzed. The results showed that the CC genotype of rs2910164 was more frequent in patients with CAD than in controls, which was associated with the risk of CAD in the study population. However, Has-miR-143 rs41291957 genotype did not show a clear correlation with the risk of CAD.
Copy Number Variation Analysis Revealed the Evolutionary Difference between Chinese Indigenous Pigs and Asian Wild Boars
Authors: Fan Shuhao , Kong Chengcheng , Chen Yige , Zheng Xianrui , Zhou Ren , Zhang Xiaodong , Wu Xudong , Zhang Wei , Ding Yueyun , Yin Zongjun
Published: February 2023

Copy number variation (CNV) has been widely used to study the evolution of different species. We first discovered different CNVs in 24 Anqingliubai pigs and 6 Asian wild boars using next-generation sequencing at the whole-genome level with 10× depth to understand the relationship between genetic evolution and production traits in wild boars and domestic pigs. A total of 97,489 CNVs were identified and divided into 10,429 copy number variation regions (CNVRs), occupying 32.06% of the porcine genome. Chromosome 1 had the most CNVRs, and chromosome 18 had the least. Ninety-six CNVRs were selected using VST 1% based on the signatures of all CNVRs, and sixty-five genes were identified in the selected regions. These genes were strongly correlated with traits distinguishing groups by enrichment in Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways, such as growth (CD36), reproduction (CIT, RLN), detoxification (CYP3A29), and fatty acid metabolism (ELOVL6). The QTL overlapping regions were associated with meat traits, growth, and immunity, which was consistent with CNV analysis. Our findings increase the understanding of evolved genome structural variations between wild boars and domestic pigs, and provide new molecular biomarkers to guide breeding and the efficient use of available genetic resources.
Primary Care Physicians’ Knowledge and Attitudes Regarding Palliative Care in Northeast Malaysia
Authors: Hamdan Norhazura , Yaacob Lili Husniati , Idris Nur Suhaila , Abdul Majid Mohd Shafik
Published: February 2023

Palliative care in Malaysia has progressed steadily since its inception in 1991, and it has been integrated gradually into primary health care in the past decade. This study aims to assess the level of knowledge and the attitudes towards palliative care and its associated factors among primary care physicians. A cross-sectional study was conducted among primary care physicians using two validated questionnaires: the Palliative Care Knowledge Test (PCKT) and Frommelt’s Attitude Toward Care of the Dying (FATCOD). The data were analysed using descriptive and linear regression statistics. A total of 241 primary care physicians from 27 different health clinics participated in the study. The mean PCKT score was 8.68 (2.94), whereas the mean FATCOD score was 106.8 (9.14). The maximum score for each questionnaire was 20 and 150, respectively. There was a significant positive relationship between knowledge and attitudes toward palliative care, with a p-value of 0.003 (CI 0.22–1.04) and an r-value of 0.42. Palliative care knowledge among primary care physicians is still low despite their overall positive attitude towards the service. This finding suggests the urgent need for more education and training on palliative care for primary care physicians in Malaysia.
Man as Image of Nature in Magnus Hundt: The Perspective of a Thomist ca. 1500
Authors: Engel Karsten
Published: February 2023

This paper draws on a late medieval example to show that images of nature can also be images of the human body. It presents the Anthropologium de hominis dignitate by the Leipzig magister Magnus Hundt (1449–1519). The Anthropologium is a text that prominently integrates the human body into its conception of man and its account of human dignity. The body is not presented as a prison of the soul, but as a perfectly balanced physical counterpart to the soul. The paper shows how Hundt’s reflections were influenced by his commitment to the Thomistic school. Moreover, it reveals how the elevated Imago Dei thesis provides a justification for the study of the human body. Hundt is shown to offer nothing less than a theological–philosophical legitimation for practising medicine. In doing so, he also incorporates images of nature in a literal sense, insofar as he includes images of the human body in his book.
Understanding the Perspectives of Latino Adults Who Smoke on Physical Activity: A Qualitative Study
Authors: Hernández-Torres Ruthmarie , Alaniz-Cantu Edgar , Bautista Rojas Maria Valeria , Lara Daimarelys , Merritt Sarah , DeJesus Elisa , D’Abundo Michelle Lee , McIntosh Scott , Ossip Deborah J. , Fuller Daniel , Rivera M. Patricia , Cupertino Ana Paula , Cartujano-Barrera Francisco
Published: February 2023

Smoking and sedentary lifestyle frequently co-occur among Latinos. Evidence suggests that moderate to vigorous physical activity (MVPA) may enhance smoking cessation rates. However, this synergistic phenomenon has not been studied among Latinos, the largest minority group in the United States. This qualitative study consisted of semi-structured interviews in English or Spanish with Latino adults who smoke (n = 20) to understand their perspectives on physical activity. Participants were recruited using community-based recruitment strategies. The Health Belief Model was used as a framework for qualitative theoretical analysis. Multiple perceived benefits (e.g., mood management, strategy to quit smoking), susceptibility (e.g., risk of cardiovascular diseases, physical impairment), and barriers (e.g., lack of social support, low financial resources) of being physically active were identified. Moreover, multiple cues to action to do physical activity (e.g., being a role model, spending time with family and friends) were identified. These factors provide concrete operational strategies to address smoking cessation and physical activity among Latinos. Further research is needed on how best to integrate these perspectives into smoking cessation interventions.