bluuebunny/crossref_metadata_2025 huggingface dataset:
Phase Transformation Kinetics of a FCC Al0.25CoCrFeNi High-Entropy Alloy during Isochronal Heating
Authors: Wang Jun , Wei Chen , Yang Haoxue , Guo Tong , Xu Tingting , Li Jinshan
Published: December 2018

The phase transformation kinetics of a face-centered-cubic (FCC) Al0.25CoCrFeNi high-entropy alloy during isochronal heating is investigated by thermal dilation experiment. The phase transformed volume fraction is determined from the thermal expansion curve, and results show that the phase transition is controlled by diffusion controlled nucleation-growth mechanism. The kinetic parameters, activation energy and kinetic exponent are determined based on Kissinger–Akahira–Sunose (KAS) and Johnson–Mehl–Avrami (JMA) method, respectively. The activation energy and kinetic exponent determined are almost constant, indicating a stable and slow speed of phase transition in the FCC Al0.25CoCrFeNi high-entropy alloy. During the main transformation process, the kinetic exponent shows that the phase transition is diffusion controlled process without nucleation during the transformation.
Bonding Strength of a Glass Microfluidic Device Fabricated by Femtosecond Laser Micromachining and Direct Welding
Authors: Kim Sungil , Kim Jeongtae , Joung Yeun-Ho , Choi Jiyeon , Koo Chiwan
Published: December 2018

We present a rapid and highly reliable glass (fused silica) microfluidic device fabrication process using various laser processes, including maskless microchannel formation and packaging. Femtosecond laser assisted selective etching was adopted to pattern microfluidic channels on a glass substrate and direct welding was applied for local melting of the glass interface in the vicinity of the microchannels. To pattern channels, a pulse energy of 10 μJ was used with a scanning speed of 100 mm/s at a pulse repetition rate of 500 kHz. After 20–30 min of etching in hydrofluoric acid (HF), the glass was welded with a pulse energy of 2.7 μJ and a speed of 20 mm/s. The developed process was as simple as drawing, but powerful enough to reduce the entire production time to an hour. To investigate the welding strength of the fabricated glass device, we increased the hydraulic pressure inside the microchannel of the glass device integrated into a custom-built pressure measurement system and monitored the internal pressure. The glass device showed extremely reliable bonding by enduring internal pressure up to at least 1.4 MPa without any leakage or breakage. The measured pressure is 3.5-fold higher than the maximum internal pressure of the conventional polydimethylsiloxane (PDMS)–glass or PDMS–PDMS bonding. The demonstrated laser process can be applied to produce a new class of glass devices with reliability in a high pressure environment, which cannot be achieved by PDMS devices or ultraviolet (UV) glued glass devices.
Lithology and U–Pb Geochronology of Basement of Cenozoic Yitong Basin in Northeastern China: Implication for Basin Architecture and New Horizon of Deep Natural Gas Exploration
Authors: Chen Zhenlin , Jiang Shu , Wang Hua , Mei Lianfu , Miao Hongbo , Zou Yu
Published: December 2018

The lithology and formation age of basement rocks are significant for the understanding of the nature of basin architecture, evolution and the potential of hydrocarbons of a basin. In this study, the basement lithology of the Cenozoic Yitong Basin is investigated through the petrological analysis of cores, cuttings, and thin sections. The results suggest that the basement rocks of the Yitong Basin are mostly composed of unique igneous rocks that are different from nearby basins’ sedimentary and metamorphic basement. The igneous rocks are dominated by intrusive monzonite granite and alkali feldspar granite. Additionally, U–Pb zircon geochronology of basement samples by LA-ICP-MS and the geological interpretation of apparent resistivity data indicate that the igneous basement in major part of the basin was mainly formed by a lateral intrusion of granite into the Permian sedimentary stratum in the Yanshanian period from 177 to 170 Ma. The results also reveal the two-layer basin architecture with coal-bearing Carboniferous–Permian strata below the igneous basement covered with Tertiary sediments, thus providing a new geologic horizon for deep natural gas exploration in the older coal-bearing sedimentary rocks beneath the current igneous basement.
Geochemistry and Mineralogy of Rare Earth Elements (REE) in Bauxitic Ores of the Catalan Coastal Range, NE Spain
Authors: Reinhardt Nils , Proenza Joaquín , Villanova-de-Benavent Cristina , Aiglsperger Thomas , Bover-Arnal Telm , Torró Lisard , Salas Ramon , Dziggel Annika
Published: December 2018

Karst bauxite deposits are currently investigated as a new resource for rare earth elements (REE) in order to avoid present and future supply shortfalls of these critical metals. The present work focuses on the geochemistry and mineralogy of the REE in karst bauxite deposits of the Catalan Coastal Range (CCR), NE-Spain. It is revealed that the studied bauxitic ores have a dominant breccia and local ooido-pisoidic and pelitomorphic texture. The bauxitic ores are mostly composed of kaolinite and hematite, as well as of lesser amounts of boehmite, diaspore, rutile and calcite. The mineralogy and major element composition indicate incomplete bauxitization of an argillaceous precursor material possibly derived from the erosion of the Mesozoic Ebro massif paleo-high. The studied bauxites are characterized by ∑REE (including Sc, Y) between 286 and 820 ppm (av. 483 ppm) and light REE to heavy REE (LREE/HREE) ratios up to 10.6. REE are mainly concentrated in phosphate minerals, identified as monazite-(Ce) and xenotime-(Y) of detrital origin and unidentified REE-phosphates of a possible authigenic origin. REE remobilization presumably took place under acidic conditions, whereas REE entrapment in the form of precipitation of authigenic rare earth minerals from percolating solutions was related to neutral to slightly alkaline conditions. During the bauxitization process no significant REE fractionation took place and the REE distribution pattern of the bauxitic ores was governed by the REE budget of the precursor material. Finally, adsorption as a main REE scavenging mechanism in the studied CCR bauxite deposits should not be considered, since the presented data did not reveal significant REE contents in Fe-and Mn-oxyhydroxides and clay minerals.
Correlation of Hydrocarbon Reservoir Sandstones Using Heavy Mineral Provenance Signatures: Examples from the North Sea and Adjacent Areas
Authors: Morton Andrew , McGill Paula
Published: December 2018

Correlation of hydrocarbon reservoir sandstones is one of the most important economic applications for heavy mineral analysis. In this paper, we review the fundamental principles required for establishing correlation frameworks using heavy mineral data, and illustrate the applications of a wide variety of heavy mineral techniques using a number of case studies from hydrocarbon reservoirs in the North Sea and adjacent areas. The examples cover Triassic red-bed successions in the central North Sea and west of Shetland, which have been subdivided and correlated using provenance-sensitive ratio data and mineral morphologies; Middle Jurassic paralic sandstones in the northern North Sea, correlated using garnet geochemistry; Upper Jurassic deep water sandstones in the northern North Sea, discriminated using rutile geochemistry and detrital zircon age data; and the “real-time” application of the technique at well site in Devonian-Carboniferous fluvio-lacustrine sandstones of the Clair Field, west of Shetland.
Initial Investigation into the Leaching of Manganese from Nodules at Room Temperature with the Use of Sulfuric Acid and the Addition of Foundry Slag—Part I
Authors: Toro Norman , Herrera Nelson , Castillo Jonathan , Torres Cynthia M. , Sepúlveda Rossana
Published: December 2018

In this study, the surface optimization methodology was used to assess the effect of three independent variables—time, particle size and sulfuric acid concentration—on Mn extraction from marine nodules during leaching with H2SO4 in the presence of foundry slag. The effect of the MnO2/Fe ratio and particle size (MnO2) was also investigated. The maximum Mn extraction rate was obtained when a MnO2 to Fe molar ratio of 0.5, 1 M of H2SO4, −320 + 400 Tyler mesh (−47 + 38 μm) nodule particle size and a leaching time of 30 min were used.
Classical QSAR and Docking Simulation of 4-Pyridone Derivatives for Their Antimalarial Activity
Authors: Flores-Sumoza Máryury , Alcázar Jackson , Márquez Edgar , Mora José , Lezama Jesús , Puello Esneyder
Published: December 2018

In this work, the minimum energy structures of 22 4-pyridone derivatives have been optimized at Density Functional Theory level, and several quantum molecular, including electronic and thermodynamic descriptors, were computed for these substrates in order to obtain a statistical and meaningful QSAR equation. In this sense, by using multiple linear regressions, five mathematical models have been obtained. The best model with only four descriptors (r2 = 0.86, Q2 = 0.92, S.E.P = 0.38) was validated by the leave-one-out cross-validation method. The antimalarial activity can be explained by the combination of the four mentioned descriptors e.g., electronic potential, dipolar momentum, partition coefficient and molar refractivity. The statistical parameters of this model suggest that it is robust enough to predict the antimalarial activity of new possible compounds; consequently, three small chemical modifications into the structural core of these compounds were performed specifically on the most active compound of the series (compound 13). These three new suggested compounds were leveled as 13A, 13B and 13C, and the predicted biological antimalarial activity is 0.02 µM, 0.03 µM, and 0.07 µM, respectively. In order to complement these results focused on the possible action mechanism of the substrates, a docking simulation was included for these new structures as well as for the compound 13 and the docking scores (binding affinity) obtained for the interaction of these substrates with the cytochrome bc1, were −7.5, −7.2, −6.9 and −7.5 kcal/mol for 13A, 13B, 13C and compound 13, respectively, which suggests that these compounds are good candidates for its biological application in this illness.
Salvia elegans, Salvia greggii and Salvia officinalis Decoctions: Antioxidant Activities and Inhibition of Carbohydrate and Lipid Metabolic Enzymes
Authors: Pereira Olívia R. , Catarino Marcelo D. , Afonso Andrea F. , Silva Artur M. S. , Cardoso Susana M.
Published: December 2018

Salvia elegans Vahl., Salvia greggii A. Gray, and Salvia officinalis L. decoctions were investigated for their health-benefit properties, in particular with respect to antioxidant activity and inhibitory ability towards key enzymes with impact in diabetes and obesity (α-glucosidase, α-amylase and pancreatic lipase). Additionally, the phenolic profiles of the three decoctions were determined and correlated with the beneficial properties. The S. elegans decoction was the most promising in regard to the antioxidant effects, namely in the scavenging capacity of the free radicals DPPH•, NO• and O2•–, and the ability to reduce Fe3+, as well as the most effective inhibitor of α-glucosidase (EC50 = 36.0 ± 2.7 μg/mL vs. EC50 = 345.3 ± 6.4 μg/mL and 71.2 ± 5.0 μg/mL for S. greggii and S. officinalis, respectively). This superior activity of the S. elegans decoction over those of S. greggii and S. officinalis was, overall, highly correlated with its richness in caffeic acid and derivatives. In turn, the S. officinalis decoction exhibited good inhibitory capacity against xanthine oxidase activity, a fact that could be associated with its high content of flavones, in particular the glycosidic forms of apigenin, scutellarein and luteolin.
Why Natural or Electron Irradiated Sheep Wool Show Anomalous Sorption of Higher Concentrations of Copper(II)
Authors: Porubská Mária , Kleinová Angela , Hybler Peter , Braniša Jana
Published: December 2018

Sorption of higher concentrations of Cu(II) solution onto natural sheep wool or wool irradiated by an electron beam was studied. Sorption isotherms were of unexpected character, showing extremes. The samples with lower absorbed doses adsorbed less than non-irradiated wool, while higher doses led to increased sorption varying with both concentration and dose. FTIR spectra taken from the fibre surface and bulk were different. It was concluded that there was formation of Cu(II)-complexes of carboxylic and cysteic acids with ligands coming from various keratin macromolecules. Clusters of chains crosslinked through the ligands on the surface limit diffusion of Cu(II) into the bulk of fibre, thus decreasing the sorption. After exhausting the available ligands on the surface the remaining Cu(II) cations diffuse into the keratin bulk. Here, depending on accessibility of suitable ligands, Cu(II) creates simple or complex salts giving rise to the sorption extremes. Suggestion of a mechanism for this phenomenon is presented.
Molecular Mechanism of Action of RORγt Agonists and Inverse Agonists: Insights from Molecular Dynamics Simulation
Authors: Sun Nannan , Yuan Congmin , Ma Xiaojun , Wang Yonghui , Gu Xianfeng , Fu Wei
Published: December 2018

As an attractive drug-target, retinoic acid receptor-related orphan receptor-gamma-t (RORγt) has been employed widely to develop clinically relevant small molecular modulators as potent therapy for autoimmune disease and cancer, but its molecular mechanism of action (MOA) remains unclear. In the present study, we designed and discovered two novel RORγt ligands that are similar in structure, but different in efficacy. Using fluorescence resonance energy transfer (FRET) assay, compound 1 was identified as an agonist with an EC50 of 3.7 μM (max. act.: 78%), while compound 2 as an inverse agonist with an IC50 value of 2.0 μM (max. inh.: 61%). We performed molecular dynamics (MD) simulations, and elucidated the MOA of RORγt agonist and inverse agonist. Through the analyses of our MD results, we found that, after RORγt is bound with the agonist 1, the side chain of Trp317 stays in the gauche- conformation, and thus helps to form the hydrogen bond, His479-Trp502, and a large hydrophobic network among H11, H11′, and H12. All these interactions stabilize the H12, and helps the receptor recruit the coactivator. When the RORγt is bound with the inverse agonist 2, the side chain of Trp317 is forced to adopt the trans conformation, and these presumed interactions are partially destroyed. Taken together, the critical role of residue Trp317 could be viewed as the driving force for the activation of RORγt.
Time-Resolved Spectroscopic Study of N,N-Di(4-bromo)nitrenium Ions in Selected Solutions
Authors: Du Lili , Lan Xin , Yan Zhiping , Zhu Ruixue , Phillips David Lee
Published: December 2018

Nitrenium ions are important reactive intermediates in chemistry and biology. In this work, femtosecond and nanosecond transient absorption (fs-TA and ns-TA) along with nanosecond time-resolved resonance Raman (ns-TR3) experiments were employed to examine the photochemical pathways of N-(4,4′-dibromodiphenylamino)-2,4,6-trimethylpyridinium BF4− (salt (DN) from just absorption of a photon of light to the production of the important N,N-di(4-bromophenyl)nitrenium ion 2. In acetonitrile (MeCN), the formation of halogenated diarylnitrenium ion 2 was observed within 4 ps, showing the vibrational spectra with strong intensity. The nucleophilic adduct reaction of ion 2 with H2O was also examined in aqueous solutions. The direct detection of the unique ortho adduct intermediate 3 shows that there is an efficient and exclusive reaction pathway for 2 with H2O. The results shown in this paper give new characterization of 2, which can be used to design time-resolved spectroscopy investigations of covalent addition reactions of nitrenium ions with other molecules in future studies.
Core-Shell Nanoencapsulation of α-Tocopherol by Blending Sodium Oleate and Rebaudioside A: Preparation, Characterization, and Antioxidant Activity
Authors: He Junbo , Shi Hao , Huang Shuangshuang , Han Lijuan , Zhang Weinong , Zhong Qixin
Published: December 2018

Nanoencapsulation of α-tocopherol (α-TOC) by blending sodium oleate (NaOl) and rebaudioside A (RebA) was successfully prepared by self-assembly method under mild conditions. The optimized nanoemulsion showed the loading capacity of α-TOC was 30 wt% of sodium oleate. FTIR analysis suggested that hydrogen bonds and hydrophobic interactions were the major forces in α-TOC-NaOl/RebA complexes that were spherical and possessed well-distinguishable core-shell structures. The freeze-dried α-TOC-NaOl/RebA complexes had great stability under ambient conditions. The release profile of α-TOC showed a first-order kinetics reaching around 67.9% after 90 h at 25 °C. Nanoencapsulation improved dispersibility and greatly increased the antioxidant activity of α-TOC. Therefore, the stable α-TOC-NaOl/RebA core-shell complexes prepared from “generally recognized as safe” (GRAS) ingredients have great potential to supplement α-TOC in food and cosmetic products.
Electrodeposition–Assisted Assembled Multilayer Films of Gold Nanoparticles and Glucose Oxidase onto Polypyrrole-Reduced Graphene Oxide Matrix and Their Electrocatalytic Activity toward Glucose
Authors: Wu Baoyan , Hou Shihua , Xue Yongyong , Chen Zhan
Published: December 2018

The study reports a facile and eco-friendly approach for nanomaterial synthesis and enzyme immobilization. A corresponding glucose biosensor was fabricated by immobilizing the gold nanoparticles (AuNPs) and glucose oxidase (GOD) multilayer films onto the polypyrrole (PPy)/reduced graphene oxide (RGO) modified glassy carbon electrode (GCE) via the electrodeposition and self-assembly. PPy and graphene oxide were first coated on the surface of a bare GCE by the electrodeposition. Then, AuNPs and GOD were alternately immobilized onto PPy-RGO/GCE electrode using the electrodeposition of AuNPs and self-assembly of GOD to obtain AuNPs-GOD multilayer films. The resulting PPy-RGO-(AuNPs-GOD)n/GCE biosensors were used to characterize and assess their electrocatalytic activity toward glucose using cyclic voltammetry and amperometry. The response current increased with the increased number of AuNPs-GOD layers, and the biosensor based on four layers of AuNPs-GOD showed the best performance. The PPy-RGO-(AuNPs-GOD)4/GCE electrode can detect glucose in a linear range from 0.2 mM to 8 mM with a good sensitivity of 0.89 μA/mM, and a detection limit of 5.6 μM (S/N = 3). This study presents a promising eco-friendly biosensor platform with advantages of electrodeposition and self-assembly, and would be helpful for the future design of more complex electrochemical detection systems.
Ionic Transportation and Dielectric Properties of YF3:Eu3+ Nanocrystals
Authors: Cui Xiaoyan , Hu Tingjing , Wang Jingshu , Zhang Junkai , Zhong Xin , Chen Yanli , Li Xuefei , Yang Jinghai , Gao Chunxiao
Published: December 2018

The ionic transportation and dielectric properties of YF3:Eu3+ nanocrystals are investigated by AC impedance spectroscopy. The ion diffusion coefficient and conductivity increase along with the doping concentration and reach their highest values at 4% of Eu3+. The difference of ionic radius between Eu3+ and Y3+ leads to the structural disorder and lattice strain, which deduces the increase of the ion diffusion coefficient and conductivity before 4% Eu3+ doping; then the interaction of the neighboring doping ions is dominated, which results in the difficulty of ion migration and decreases of the ion diffusion coefficient and conductivity. The strong dispersion of the permittivity in the low frequency region indicates that the charge carrier transport mechanism is the ion hopping in the system. The low-frequency hopping dispersion is affected by an interfacial polarization, which exhibits a Maxwell-Wagner relaxation process, and its loss peak shifts to higher frequency with the ionic conductivity increasing.
Effect of Dimensions and Agglomerations of Carbon Nanotubes on Synchronous Enhancement of Mechanical and Damping Properties of Epoxy Nanocomposites
Authors: Wang Tingting , Song Bo , Qiao Kun , Huang Yudong , Wang Li
Published: December 2018

In order to achieve simultaneous enhancement of mechanical and damping properties, epoxy resin nanocomposites reinforced with a series of carboxylic multi-walled carbon nanotubes (C-MWCNTs) with different dimensions were prepared. A solution-based high-speed shear dispersion method was developed. The dispersion mechanism of carbon nanotubes was studied, and the degree of dispersion difficulty of carbon nanotubes with different dimensions was evaluated by theoretical calculation, and the minimum size of agglomerates for dispersion based on the mechanism of rupture was deduced. Then, the effect of synchronous enhancement on the mechanical and damping properties was tested by experiment. The effects of dimensions and agglomerations on the tensile properties, damping properties, and glass transition temperature (Tg) of the nanocomposites were investigated. The ranking of dispersion difficulty was verified using the deviations between predicted and experimental tensile modulus. The experimental results showed that the effects of synchronous enhancement on the mechanical properties and damping capacity of two kinds of specimens were remarkable and the only drawback was that their Tg showed the maximum decrease. Further studies indicated that C-MWCNTs with large aspect ratios and large specific surface areas possessed better effects on synchronous enhancement, but caused a decrease in the glass transition temperature, while agglomeration had the opposite effect. The results of this work would be helpful for preparing improved structural damping integrated composites.
Energy-Dense Diets and Mineral Metabolism in the Context of Chronic Kidney Disease–Metabolic Bone Disease (CKD-MBD)
Authors: Rodriguez Mariano , Aguilera-Tejero Escolastico
Published: December 2018

The aim of this paper is to review current knowledge about the interactions of energy-dense diets and mineral metabolism in the context of chronic kidney disease–metabolic bone disease (CKD-MBD). Energy dense-diets promote obesity and type II diabetes, two well-known causes of CKD. Conversely, these diets may help to prevent weight loss, which is associated with increased mortality in advanced CKD patients. Recent evidence indicates that, in addition to its nephrotoxic potential, energy-dense food promotes changes in mineral metabolism that are clearly detrimental in the context of CKD-MBD, such as phosphorus (P) retention, increased concentrations of fibroblast growth factor 23, decreased levels of renal klotho, and reduction in circulating concentrations of calcitriol. Moreover, in uremic animals, a high fat diet induces oxidative stress that potentiates high P-induced vascular calcification, and these extraskeletal calcifications can be ameliorated by oral supplementation of vitamin E. In conclusion, although energy-dense foods may have a role in preventing undernutrition and weight loss in a small section of the CKD population, in general, they should be discouraged in patients with renal disease, due to their impact on P load and oxidative stress.
Plant-Based Diets: Considerations for Environmental Impact, Protein Quality, and Exercise Performance
Authors: Lynch Heidi , Johnston Carol , Wharton Christopher
Published: December 2018

Plant-based diets provide well-established physical and environmental health benefits. These benefits stem in part from the degree of restriction of animal-derived foods. Historically, meat and other animal-derived proteins have been viewed as an integral component of athletes’ diets, leading some to question the adequacy of vegetarian or vegan diets for supporting athletic performance. The purpose of this review is to examine the impact of plant-based diets on human physical health, environmental sustainability, and exercise performance capacity. Based on currently available literature, it is unlikely that plant-based diets provide advantages, but do not suffer from disadvantages, compared to omnivorous diets for strength, anaerobic, or aerobic exercise performance. However, plant-based diets typically reduce the risk of developing numerous chronic diseases over the lifespan and require fewer natural resources for production compared to meat-containing diets. As such, plant-based diets appear to be viable options for adequately supporting athletic performance while concurrently contributing to overall physical and environmental health. Given the sparse literature comparing omnivore, vegetarian, and vegan athletes, particularly at the elite level, further research is warranted to ascertain differences that might appear at the highest levels of training and athletic performance.
Nutritional Quality of Lunches Served in South East England Hospital Staff Canteens
Authors: Jaworowska Agnieszka , Rotaru Gabriela , Christides Tatiana
Published: December 2018

Worksite canteens generally are characterized by obesogenic environments, which offer access to energy-dense foods and sugar-sweetened beverages rather than nutrient-rich food. This study assessed the nutritional quality of hot lunches offered in National Health Service (NHS) hospital staff canteens: 35 side dishes and 112 meals were purchased from 8 NHS hospital staff canteens. The meals were analyzed for portion size, energy, protein, total fat, saturated fatty acids (SFAs), salt, and the sodium to potassium ratio. The vegetarian and meat-based lunch meals served in the hospital staff canteens tended to be high in energy, total fat, saturated fatty acids, and salt: 40%, 59% and 67% of meat meals and 34%, 43%, and 80% of vegetarian meals were assigned the red traffic light label for total fat, salt, and SFAs per portion, respectively. Similar types of meals, but served in different hospitals, varied considerably in their nutritional quality. The consumption of some lunch meals could provide more than 50% of recommended total fat, SFAs, and salt for both men and women and daily energy for women. The majority of analyzed lunch meals were characterized by an unfavorable nutrient profile, and regular consumption of such meals may increase the risk of noncommunicable diseases.
Reproducibility of a Questionnaire for Dietary Habits, Lifestyle and Nutrition Knowledge Assessment (KomPAN) in Polish Adolescents and Adults
Authors: Kowalkowska Joanna , Wadolowska Lidia , Czarnocinska Jolanta , Czlapka-Matyasik Magdalena , Galinski Grzegorz , Jezewska-Zychowicz Marzena , Bronkowska Monika , Dlugosz Anna , Loboda Dorota , Wyka Joanna
Published: December 2018

The aim of the study was to evaluate the reproducibility of the Dietary Habits and Nutrition Beliefs Questionnaire (KomPAN) in Polish adolescents and adults, including the assessment of indexes developed based on the questionnaire. In total, the study involved 954 subjects aged 15–65 (53.9% females). Interviews using the interviewer-administered questionnaire (IA-Q) in healthy subjects (n 299) and the self-administered questionnaire (SA-Q) in healthy subjects (n 517) and outpatients (n 138) were conducted and repeated after two weeks. Considering the consumption frequency of 33 food items, the cross-classification (test-retest) agreement of classification into the same category obtained for IA-Q in healthy subjects ranged from 72.2% (fruit juices) to 91.6% (energy drinks); the kappa statistic was >0.60 for all food items. For SA-Q conducted in healthy subjects the cross-classification agreement ranged from 63.8% (vegetable oils, margarines, mixes of butter and margarines) to 84.7% (lard); the kappa statistic was >0.50 for all food items. For SA-Q in outpatients, the cross-classification agreement ranged from 42.0% (both fruit juices and white rice, white pasta, fine-ground groats) to 92.0% (energy drinks); the kappa statistic was ≥0.40 for 20/33 food items. The kappa statistic for lifestyle items ranged 0.42–0.96, and for the nutrition knowledge level it ranged 0.46–0.73. The questionnaire showed moderate to very good reproducibility and can be recommended to assess dietary habits, lifestyle and nutrition knowledge of healthy adolescents and adults and those suffering from chronic diseases, after validation and/or calibration study is carried out. The reproducibility of the interviewer-administered questionnaire was better than its self-administered version. The reproducibility of the self-administered questionnaire was better in healthy subjects than in outpatients.
Selenium Status Is Not Associated with Cognitive Performance: A Cross-Sectional Study in 154 Older Australian Adults
Authors: Cardoso Barbara R. , Szymlek-Gay Ewa A. , Roberts Blaine R. , Formica Melissa , Gianoudis Jenny , O’Connell Stella , Nowson Caryl A. , Daly Robin M.
Published: December 2018

Selenium was suggested to play a role in modulating cognitive performance and dementia risk. Thus, this study aimed to investigate the association between selenium status and cognitive performance, as well as inflammatory and neurotrophic markers in healthy older adults. This cross-sectional study included 154 older adults (≥60 years) from Victoria, Australia. Participants were assessed for cognitive performance (Cogstate battery), dietary selenium intake (two 24-h food recalls), plasma selenium concentration, inflammatory markers (interleukin (IL)-6, -8, -10, tumor necrosis factor-alpha and adiponectin) and neurotrophic factors (brain-derived neurotrophic factor, vascular endothelial growth factor and insulin-like growth factor 1). Dietary selenium intake was adequate for 85% of all participants. The prevalence of selenium deficiency was low; only 8.4% did not have the minimum concentration in plasma required for optimization of iodothyronine 5′ deiodinases activity. Multiple linear regression analysis revealed that plasma selenium was not associated with cognitive performance, inflammatory markers nor neurotrophic factors, independent of age, sex, body mass index (BMI), habitual physical activity, APOE status, education, and history of cardiovascular disease. The lack of association might be due to the optimization of selenoproteins synthesis as a result of adequate selenium intake. Future prospective studies are recommended to explore potential associations of selenium status with age-associated cognitive decline.
Lipidomic Response to Coffee Consumption
Authors: Kuang Alan , Erlund Iris , Herder Christian , Westerhuis Johan A. , Tuomilehto Jaakko , Cornelis Marilyn C.
Published: December 2018

Coffee is widely consumed and contains many bioactive compounds, any of which may impact pathways related to disease development. Our objective was to identify individual lipid changes in response to coffee drinking. We profiled the lipidome of fasting serum samples collected from a previously reported single blinded, three-stage clinical trial. Forty-seven habitual coffee consumers refrained from drinking coffee for 1 month, consumed 4 cups of coffee/day in the second month and 8 cups/day in the third month. Samples collected after each coffee stage were subject to quantitative lipidomic profiling using ion-mobility spectrometry–mass spectrometry. A total of 853 lipid species mapping to 14 lipid classes were included for univariate analysis. Three lysophosphatidylcholine (LPC) species including LPC (20:4), LPC (22:1) and LPC (22:2), significantly decreased after coffee intake (p < 0.05 and q < 0.05). An additional 72 species mapping to the LPC, free fatty acid, phosphatidylcholine, cholesteryl ester and triacylglycerol classes of lipids were nominally associated with coffee intake (p < 0.05 and q > 0.05); 58 of these decreased after coffee intake. In conclusion, coffee intake leads to lower levels of specific LPC species with potential impacts on glycerophospholipid metabolism more generally.
Impact of Nutritional Status on Gastroenteropancreatic Neuroendocrine Tumors (GEP-NET) Aggressiveness
Authors: Barrea Luigi , Altieri Barbara , Muscogiuri Giovanna , Laudisio Daniela , Annunziata Giuseppe , Colao Annamaria , Faggiano Antongiulio , Savastano Silvia
Published: December 2018

Neuroendocrine tumors (NETs) are rare neoplasms mostly originating from the gastroenteropancreatic tract (GEP-NETs). Data regarding nutritional status in GEP-NET patients are limited. The aim of the study was to investigate the nutritional status and adherence to the Mediterranean Diet (MD) in GEP-NET patients and to correlate them with tumor aggressiveness. A cross-sectional case-control observational study was conducted enrolling 83 patients with well-differentiated G1/G2 GEP-NETs after resection, as well as 83 healthy subjects, age, sex and body mass index-matched. Nutritional status was assessed by evaluating with Bioelectrical Impedance analysis and its phase angle (PhA), adherence to the MD according to PREDIMED score, dietary assessment, anthropometric parameters, and clinico-pathological characteristics. GEP-NET patients consumed less frequently vegetables, fruits, wine, fish/seafood, nuts, and more frequently red/processed meats, butter, cream, margarine, and soda drinks than controls. Patients with more aggressive disease presented a lower adherence to MD according to PREDIMED categories in comparison to G1, localized and free/stable disease status. A smaller PhA value and a lower PREDIMED score were significantly correlated with G2 tumor, metastases, and progressive disease. To the best of our knowledge, this is the first study reporting an association between nutritional status and tumor aggressiveness in a selected group of GEP-NETs. Moreover, higher intakes of food of MD, may represent a potential tool for prevention of tumor aggressiveness. Thus, a skilled nutritionist should be an integral part of the multidisciplinary management of GEP-NET patients.
Pistachio Consumption Prevents and Improves Lipid Dysmetabolism by Reducing the Lipid Metabolizing Gene Expression in Diet-Induced Obese Mice
Authors: Terzo Simona , Caldara Gaetano Felice , Ferrantelli Vincenzo , Puleio Roberto , Cassata Giovanni , Mulè Flavia , Amato Antonella
Published: December 2018

Pistachios contain beneficial substances such as unsaturated fatty acids, phytosterols, and polyphenols. In the present study, we investigated if pistachio consumption is able to prevent or to revert hyperglycemia, dyslipidemia, hepatic steatosis, and adipose tissue morphological alterations caused by high fat diet (HFD) in the mouse. Moreover, the impact of pistachio intake on the mRNA expression of peroxisome proliferator-activated receptor γ (PPAR-γ), fatty acid transport proteins (FAT-P), fatty acid synthase (FAS), stearoyl-CoA desaturase (SCD1), and sterol regulatory element-binding transcription factor-1c (SREBP-1c) in liver and adipose tissue was also analyzed. No change in body weight, food intake, and hyperglycemia was observed between mice consuming pistachios (HFD-P) and HFD mice. Pistachio intake was able to prevent but not to reverse HFD-induced hypertriglyceridemia. Cholesterol plasma levels, steatosis grading, body fat mass, and adipocyte size were significantly lower in HFD-P group compared to HFD in both prevention and reversal protocol. Pistachio-diet was able to prevent HFD-induced overexpression of PPAR-γ, FAS, and SCD1 in the liver and SREBP-1c, PPAR-γ, and FAT-P in adipose tissue. Similarly, HFD-P significantly ameliorated the expression levels of FAT-P and SCD1 in the liver and SREBP-1c, FAS, and SCD1 in adipose tissue of obese mice. The present study shows that pistachio consumption is able to prevent and to ameliorate obesity-related dysfunctions by positively modulating the expression of genes linked to lipid metabolism.
The Effect of a High-Dose Vitamin B Multivitamin Supplement on the Relationship between Brain Metabolism and Blood Biomarkers of Oxidative Stress: A Randomized Control Trial
Authors: Ford Talitha C. , Downey Luke A. , Simpson Tamara , McPhee Grace , Oliver Chris , Stough Con
Published: December 2018

A diet rich in B-group vitamins is essential for optimal body and brain function, and insufficient amounts of such vitamins have been associated with higher levels of neural inflammation and oxidative stress, as marked by increased blood plasma homocysteine. Neural biomarkers of oxidative stress quantified through proton magnetic spectroscopy (1H-MRS) are not well understood, and the relationship between such neural and blood biomarkers is seldom studied. The current study addresses this gap by investigating the direct effect of 6-month high-dose B-group vitamin supplementation on neural and blood biomarkers of metabolism. Using a randomized, double-blind, placebo-controlled design, 32 healthy adults (20 female, 12 male) aged 30–65 years underwent blood tests (vitamin B6, vitamin B12, folate, and homocysteine levels) and 1H-MRS of the posterior cingulate cortex (PCC) and dorsolateral prefrontal cortex (DLPFC) before and after supplementation. Results confirmed the supplement was effective in increasing vitamin B6 and vitamin B12 levels and reducing homocysteine, whereas there was no change in folate levels. There were significant relationships between vitamin B6 and N-acetylaspartate (NAA), choline, and creatine, as well as between vitamin B12 and creatine (ps < 0.05), whereas NAA in the PCC increased, albeit not significantly (p > 0.05). Together these data provide preliminary evidence for the efficacy of high-dose B-group supplementation in reducing oxidative stress and inflammation through increasing oxidative metabolism. It may also promote myelination, cellular metabolism, and energy storage.
Magnesium: Are We Consuming Enough?
Authors: Razzaque Mohammed S.
Published: December 2018

Magnesium is essential for maintaining normal cellular and organ function. In-adequate magnesium balance is associated with various disorders, such as skeletal deformities, cardiovascular diseases, and metabolic syndrome. Unfortunately, routinely measured serum magnesium levels do not always reflect total body magnesium status. Thus, normal blood magnesium levels eclipse the wide-spread magnesium deficiency. Other magnesium measuring methods, including the magnesium loading test, may provide more accurate reflections of total body magnesium status and thus improve identification of magnesium-deficient individuals, and prevent magnesium deficiency related complications.
Preliminary Validation of the Exercise-Snacking Licensing Scale: Rewarding Exercise with Unhealthy Snack Foods and Drinks
Authors: West Jessica S. , Guelfi Kym J. , Dimmock James A. , Jackson Ben
Published: December 2018

There is evidence that individuals’ compensatory health beliefs may be an important psychological driver of health behavior. Only recently, however, have researchers begun to develop and seek to validate instruments that are suited to measuring specific pairings of the diverse compensatory health beliefs that exist. The aim of this study was to provide support for key aspects of validity associated with the Exercise-Snacking Licensing Scale (ESLS), an instrument that was designed to assess individuals’ endorsement (or licensing) of unhealthy snacking behaviors around exercise. Participants (N = 1095) responded to a version of the ESLS that was designed to assess their licensing responses following either “light” or “tiring” physical activity, and completed additional instruments assessing dispositional, exercise-related, and diet-related constructs. Analyses indicated that scores derived from both versions of the ESLS (“light” and “tiring” physical activity) displayed a relatively consistent factor structure, favorable alpha coefficients, and meaningful correlations with variables that are theoretically aligned with licensing. Factor analytic procedures did, however, indicate that researchers may wish, in future, to consider the use (or not) of reverse-scored items within the ESLS. Together, these findings provide important insight into the structural, external, and generalizability aspects of validity for scores derived from the ESLS, and indicate that the ESLS may be a valuable instrument for the brief assessment of unhealthy licensing beliefs around exercise. Further use of the ESLS is encouraged to determine if and how these licensing beliefs actually influence subsequent snacking behaviors, and the potential downstream effects these beliefs may have in shaping health outcomes associated with exercise participation.
Dietary Advanced Glycation Endproducts Induce an Inflammatory Response in Human Macrophages in Vitro
Authors: Van der Lugt Timme , Weseler Antje R. , Gebbink Wouter A. , Vrolijk Misha F. , Opperhuizen Antoon , Bast Aalt
Published: December 2018

Advanced glycation endproducts (AGEs) can be found in protein- and sugar-rich food products processed at high temperatures, which make up a vast amount of the Western diet. The effect of AGE-rich food products on human health is not yet clear and controversy still exists due to possible contamination of samples with endotoxin and the use of endogenous formed AGEs. AGEs occur in food products, both as protein-bound and individual molecules. Which form exactly induces a pro-inflammatory effect is also unknown. In this study, we exposed human macrophage-like cells to dietary AGEs, both in a protein matrix and individual AGEs. It was ensured that all samples did not contain endotoxin concentrations > 0.06 EU/mL. The dietary AGEs induced TNF-alpha secretion of human macrophage-like cells. This effect was decreased by the addition of N(ε)-carboxymethyllysine (CML)-antibodies or a receptor for advanced glycation endproducts (RAGE) antagonist. None of the individual AGEs induce any TNF-alpha, indicating that AGEs should be bound to proteins to exert an inflammatory reaction. These findings show that dietary AGEs directly stimulate the inflammatory response of human innate immune cells and help us define the risk of regular consumption of AGE-rich food products on human health.
Vitamin D Supplementation Modestly Reduces Serum Iron Indices of Healthy Arab Adolescents
Authors: Masoud Mohammad S. , Alokail Majed S. , Yakout Sobhy M. , Khattak Malak Nawaz K. , AlRehaili Marwan M. , Wani Kaiser , Al-Daghri Nasser M.
Published: December 2018

Vitamin D deficiency has been shown to affect iron status via decreased calcitriol production, translating to decreased erythropoiesis. The present study aimed to determine for the first time whether vitamin D supplementation can affect iron levels among Arab adolescents. A total of 125 out of the initial 200 Saudi adolescents with vitamin D deficiency (serum 25(OH)D < 50 nmol/L) were selected from the Vitamin D-School Project of King Saud University in Riyadh, Saudi Arabia. Cluster randomization was done in schools, and students received either vitamin D tablets (1000 IU/day) (N = 53, mean age 14.1 ± 1.0 years) or vitamin D-fortified milk (40IU/200mL) (N = 72, mean age 14.8 ± 1.4 years). Both groups received nutritional counseling. Anthropometrics, glucose, lipids, iron indices, and 25(OH)D were measured at baseline and after six months. Within group analysis showed that post-intervention, serum 25(OH)D significantly increased by as much as 50%, and a parallel decrease of −42% (p-values <0.001 and 0.002, respectively) was observed in serum iron in the tablet group. These changes were not observed in the control group. Between-group analysis showed a clinically significant increase in serum 25(OH)D (p = 0.001) and decrease in iron (p < 0.001) in the tablet group. The present findings suggest a possible inhibitory role of vitamin D supplementation in the iron indices of healthy adolescents whose 25(OH)D levels are sub-optimal but not severely deficient, implying that the causal relationship between both micronutrients may be dependent on the severity of deficiency, type of iron disorder, and other vascular conditions that are known to affect hematologic indices. Well-designed, randomized trials are needed to confirm the present findings.
New Protocol for Production of Reduced-Gluten Wheat Bread and Pasta and Clinical Effect in Patients with Irritable Bowel Syndrome: A randomised, Double-Blind, Cross-Over Study
Authors: Calasso Maria , Francavilla Ruggiero , Cristofori Fernanda , De Angelis Maria , Gobbetti Marco
Published: December 2018

It has been suggested that sourdough fermented products have beneficial health effects. Fungal proteases and selected sourdough lactic acid bacteria were used to produce wheat bread and pasta with a reduced-gluten content (<50% of traditional products). Fermentable oligo-, di- and mono- saccharides and polyols and amylase/trypsin inhibitors were also evaluated. The sensorial features of new products were similar to traditional ones. The efficacy of these new products in reducing the severity of symptoms in Irritable Bowel Syndrome (IBS) patients were compared to traditional bread and pasta using a randomized, crossover-controlled trial. While on a strict gluten-free diet, patients were randomized to consume a reduced- or normal-gluten diet for 2weeks; then, patients from both arms started the wash-out period of one week, and subsequently started the final 2-week period on a normal or reduced-gluten diet. Compared to normal-gluten content, the administration of a reduced-gluten content diet resulted in a decrease of the Visual Analogue Scale score (p = 0.042), while no differences were found in the IBS-Severity Score, Hospital Anxiety and Depression Scale, and IBS-Quality of Life. Data herein reported are novel encouraging findings that should spur a new avenue of research aiming to develop products specifically designed for IBS patients.
Cross-Sectional and Longitudinal Association between Glycemic Status and Body Composition in Men: A Population-Based Study
Authors: Almusaylim Khaleal , Minett Maggie , Binkley Teresa L. , Beare Tianna M. , Specker Bonny
Published: December 2018

This study sought to evaluate the associations between changes in glycemic status and changes in total body (TB), trunk, and appendicular fat (FM) and lean mass (LM) in men. A population-based study of men aged 20–66 years at baseline were included in cross-sectional (n = 430) and three-year longitudinal (n = 411) analyses. Prediabetes was defined as fasting glucose 100–125 mg/dL. Type 2 diabetes (T2D) was determined by: self-reported diabetes, current anti-diabetic drug use (insulin/oral hypoglycemic agents), fasting glucose (≥126 mg/dL), or non-fasting glucose (≥200 mg/dL). Body composition was evaluated by dual-energy X-ray absorptiometry. Longitudinal analyses showed that changes in TB FM and LM, and appendicular LM differed among glycemic groups. Normoglycemic men who converted to prediabetes lost more TB and appendicular LM than men who remained normoglycemic (all, p < 0.05). Normoglycemic or prediabetic men who developed T2D had a greater loss of TB and appendicular LM than men who remained normoglycemic (both, p < 0.05). T2D men had greater gains in TB FM and greater losses in TB and appendicular LM than men who remained normoglycemic (all, p < 0.05). Dysglycemia is associated with adverse changes in TB and appendicular LM.