bluuebunny/crossref_metadata_2025 huggingface dataset:
Do circulating neutrophil extracellular traps predict recurrence in early breast cancer?
Authors: Martinez-Cannon Bertha Alejandra , Garcia-Ronquillo Karen , Rivera-Franco Monica M. , Leon-Rodriguez Eucario
Published: January 2023

Background

Neutrophil extracellular traps (NETs), three-dimensional structures formed by neutrophil enzymes such as neutrophil elastase (NE) and nuclear components (DNA), have been associated with progression and metastasis in breast cancer (BC). Thus, the aim of this study was to evaluate the association of circulating NETs with clinicopathological characteristics and outcomes in early BC.

Methods

A prospective cohort included women with newly diagnosed early BC. NETs were defined as the presence of NE-DNA complexes in plasma, measured by optical density. Levels of NETs were dichotomized according to the median, as low and high levels of circulating NETs. Fisher’s exact test was used to evaluate associations between NETs and clinicopathological characteristics and outcomes. Survival was assessed using the Kaplan Meier method and log-rank test.

Results

Forty patients were included, 23 (57.5%) patients with low and 17 (42.5%) with high levels of circulating NETs. No associations were found between clinicopathological characteristics and circulating NETs levels. Recurrence (p = 0.99) and site of recurrence (p = 0.99) were not statistically associated with plasma NETs levels. Overall, recurrence-free survival was not statistically different between circulating levels of NETs.

Conclusions

With a short follow-up and low number of events, our results suggest that circulating levels of NETs at diagnosis of early BC are not associated with more aggressive clinicopathological characteristics, recurrence, or site of recurrence.
Presentation and management outcomes of Retinoblastoma among Syrian refugees in Jordan
Authors: Yousef Yacoub A. , Abu Salim Qusai F. , Mohammad Mona , Jaradat Imad , Mehyar Mustafa , AlJabari Reem , Al-Habahbeh Omar , Saboubeh Khalid , Halalsheh Hadeel , Khzouz Jakub , Shawagfeh Munir , Sultan Iyad , AlMasri Mahmoud , Al-Nawaiseh Ibrahim , Al-Hussaini Maysa , Mansour Asem
Published: January 2023

Purpose

The humanitarian crisis in Syria has had a profound impact on the entire region. In this study, we report the patterns of presentation and management outcomes of Syrian patients with Retinoblastoma (Rb) treated at a single tertiary cancer center in Jordan.

Methods and Materials

This is a retrospective comparative study of Syrian refugees and Jordanian citizens who had Rb between 2011 and 2020. Collected data included patient demographics, presentation, tumor stage, treatment modalities, eye salvage rate, metastasis, and mortality.

Results

Thirty Syrian refugees (16 (53%) had bilateral disease) and 124 Jordanian citizens (51(41%) had bilateral disease) were diagnosed with Rb during this period. The median age at diagnosis for refugees was 10 and 32 months for patients with bilateral and unilateral Rb consecutively, compared to 6 and 28 months for citizens. The median lag time between signs of disease and initiation of treatment was 3 months for refugees, compared to 1 month for citizens.

Refugees were more likely to present with a more advanced stage (p=0.046). Out of 46 affected eyes in refugees; 32 (70%) eyes were group D or E, while out of 175 affected eyes among citizens; 98 (56%) eyes were group D or E. Therefore, refugees with Rb were more likely to mandate primary enucleation (48%) compared to citizens (25%) (p=0.003). However, out of 24 eyes among refugees who received conservative therapy, 15 (62%) eyes were successfully salvaged, while out of 131 affected eyes among citizens who received conservative therapy, 105 (80%) eyes were successfully salvaged (p=0.06). Two (7%) of the refugees and four (3.2%) of the citizens with Rb died from metastasis.

Conclusion

Syrian refugees with Rb presented with more advanced disease due to delay in diagnosis and referral that increased the treatment burden by decreasing the chance for eye globe salvage. However, patients who received the timely intervention had a similar outcome to citizens with Rb; probably a reflection of the management of all patients at a single specialized center. We advocate for the timely referral of refugees with this rare life-threatening tumor to a specialized cancer center for the best possible outcome.
Semaphorins and their receptors in pancreatic cancer: Mechanisms and therapeutic opportunities
Authors: Liu Dahai , Li Jie , Qi Fei , You Hua
Published: January 2023

Pancreatic cancer (PC) is a malignant tumor with high malignancy that is difficult to diagnose and treat. PC is a major medical problem because of its low early diagnosis rate, high surgical mortality rate, low cure rate, and expensive related testing cost. Therefore, the significance of finding new markers for PC is self-evident. Semaphorins (Semas) have been shown to affect angiogenesis and lymphangiogenesis and can also directly affect the behavior of tumor cells. The expression and related action targets of its family members on PC are summarized in this review.
Identification of lymph node metastasis-related genes and patterns of immune infiltration in colon adenocarcinoma
Authors: Zhang Haoxiang , Zhao Guibin , Zhu Guangwei , Ye Jianxin
Published: January 2023

Backgrounds

Colon adenocarcinoma(COAD) is one of the most common tumors of the digestive tract. Lymph node metastasis (LNM) is a well-established prognostic factor for COAD. The mechanism of COAD lymph node metastasis in immunology remains unknown. The identification of LNM-related biomarkers of COAD could help in its treatment. Thus, the current study was aimed to identify key genes and construct a prognostic signature.

Methods

Gene expression and clinical data were obtained from The Cancer Genome Atlas (TCGA) database. Differentially expressed genes were calculated by using R software. GO functional and KEGG pathway enrichment analysis were processed. The CIBERSORT algorithm was used to assess immune cell infiltration. STRING database was used to screen key genes and constructed a protein-protein interaction network (PPI network). The LASSO-Cox regression analysis was performed based on the components of the PPI network. The correlation analysis between LNM-related signature and immune infiltrating cells was then investigated. TISIDB was used to explore the correlation between the abundance of immunomodulators and the expression of the inquired gene.

Results

In total, 394 differentially expressed genes were identified. After constructing and analyzing the PPI network, 180 genes were entered into the LASSO-Cox regression model, constructing a gene signature. Five genes(PMCH, LRP2, NAT1, NKAIN4, and CD1B) were identified as LNM-related genes of clinical value. Correlation analysis revealed that LRP2 and T follicular helper cells (R=0.34, P=0.0019) and NKAIN4 and T follicular helper cells (R=0.23, P=0.041) had significant correlations. Immunologic analysis revealed that LRP2 and NKAIN4 are potential coregulators of immune checkpoints in COAD.

Conclusion

In general, this study revealed the key genes related to lymph node metastasis and prognostic signature. Several potential mechanisms and therapeutic and prognostic targets of lymph node metastasis were also demonstrated in COAD.
Prediction of necrotizing enterocolitis in very low birth weight infants by superior mesenteric artery ultrasound of postnatal day 1: A nested prospective study
Authors: Yue Guang , Wang Jun , Yang Sheng , Deng Ying , Wen Yang , Jia Wen , Cao Huiling , Ju Rong , Shi Yuan
Published: January 2023

Background

Necrotizing enterocolitis (NEC) is a devastating intestinal complication that occurs mainly in very-low-birth-weight infants (VLBWI). The study's aim was to investigate the possibility of early prediction of NEC on postnatal day 1 based on superior mesenteric artery (SMA) doppler ultrasonograpy.

Methods

A prospective, observational, nested case control study (ChiCTR1900026197) was conducted to enroll VLBWIs (birth weight <1,500 grams) between October 2019 and September 2021. Doppler ultrasound measurement was done during the first 12 h of life and before first feeding. Infants developing NEC (stage II or III) subsequently were included in NEC group and infants spare of NEC were included in control group.

Results

370 VLBWIs were enrolled (30 NEC cases). Among the ultrasound parameters, S/D was significantly higher in the NEC group (OR: 2.081, 95% CI: 1.411–3.069, P = 0.000). The area under the receiver operating curve (AUROC) following the Logistic regression was 0.704 (95% CI: 0.566–0.842, P = 0.001). The sensitivity of S/D for predicting NEC was 52.2% and the specificity was 92.7%. The critical value of S/D was 6.944 and Youden index was 0.449. Preplanned subgroup analysis confirmed that NEC infants of different stages were characterized by different SMA bloodstream. Small for gestational age (SGA) might be a confounding factor affecting intestinal bloodflow. And infants with delayed initiation or slow advancement of feeding exhibited characteristic intestinal perfusion.

Conclusions

In VLBWI, early SMA ultrasound shows the potential to predict NEC. It is reasonable to speculate that SMA bloodstream is related to intestinal structural and functional integrity.
A novel risk model based on anoikis: Predicting prognosis and immune infiltration in cutaneous melanoma
Authors: Zhou Yi , Wang Chen , Chen Yifang , Zhang Wei , Fu Zailin , Li Jianbo , Zheng Jie , Xie Minghua
Published: January 2023

Cutaneous melanoma (CM) is a highly aggressive malignancy with a dimal prognosis and limited treatment options. Anoikis is believed to involve in the regeneration, migration, and metastasis of tumor. The exact role of anoikis-related genes (ARGs) in the development and progression of cutaneous melanoma, however, remains elusive. Four ARGs (SNAI2, TFDP1, IKBKG, and MCL1) with significant differential expression were selected through Cox regression and LASSO analyses. Data for internal and external cohorts validated the accuracy and clinical utility of the prognostic risk model based on ARGs. The Kaplan–Meier curve indicated a much better overall survival rate of low-risk patients. Notably, we also found that the action of ARGs in the CM was mediated by immune-related signaling pathways. Consensus clustering and TIME landscape analysis also indicated that the low-risk score patients have excellent immune status. Moreover, the results of immunotherapy response and drug sensitivity also confirmed the potential implications of informing individualized immune therapeutic strategies for CM. Collectively, the predictive risk model constructed based on ARGs provides an excellent and accurate prediction tool for CM patients. This present research provides a rationale for the joint application of targeted therapy and immunotherapy in CM treatment. The approach could have great therapeutic value and make a contribution to personalized medicine therapy.
Mechanism of pyroptosis in neurodegenerative diseases and its therapeutic potential by traditional Chinese medicine
Authors: Liao Yanfang , Wang Xue , Huang Liting , Qian Hu , Liu Wei
Published: January 2023

Neurodegenerative diseases (NDs) are disorders characterized by degenerative degeneration of neurons and loss of their function. NDs have a complicated pathophysiology, of which neuroinflammation and neuronal death are significant factors. The inflammatory process known as pyroptosis (“fiery death”) is caused by a family of pore-forming proteins called Gasdermins (GSDMs), which appears downstream from the activation of the inflammasome. Clear evidence of enhanced pyroptosis-related proteins activity in common NDs has coincided with abnormal aggregation of pathological proteins (such as Aβ, tau, α-synuclein et al.), making pyroptosis an attractive direction for the recent study of NDs. The purpose of this review is to provide an overview of the molecular mechanisms driving pyroptosis, the mechanistic links between pyroptosis and NDs, and emerging therapeutic strategies in Traditional Chinese Medicine (TCM) to inhibit pyroptosis for the treatment of NDs.
Conduction system pacing for cardiac resynchronization therapy: State of the art, current controversies, and future perspectives
Authors: Bressi Edoardo , Grieco Domenico , Luermans Justin , Burri Haran , Vernooy Kevin
Published: January 2023

Biventricular pacing (BVP) is the established treatment to perform cardiac resynchronization therapy (CRT) in patients with heart failure (HF) and left bundle branch block (LBBB). However, BVP is an unnatural pacing modality still conditioned by the high percentage of non-responders and coronary sinus anatomy. Conduction system pacing (CSP)—His bundle pacing (HBP) and Left bundle branch area pacing (LBBAP)- upcomes as the physiological alternative to BVP in the quest for the optimal CRT. CSP showed promising results in terms of better electro-mechanical ventricular synchronization compared to BVP. However, only a few randomized control trials are currently available, and technical challenges, along with the lack of information on long-term clinical outcomes, limit the establishment of a primary role for CSP over conventional BVP in CRT candidates. This review provides a comprehensive literature revision of potential applications of CSP for CRT in diverse clinical scenarios, underlining the current controversies and prospects of this technique.
Genome-wide identification of the B3 gene family in soybean and the response to melatonin under cold stress
Authors: Ren Chunyuan , Wang Huamei , Zhou Zhiheng , Jia Jingrui , Zhang Qi , Liang Changzhi , Li Wanting , Zhang Yuxian , Yu Gaobo
Published: January 2023

Introduction

Melatonin is a multipotent molecule that exists widely in animals and plants and plays an active regulatory role in abiotic stresses. The B3 superfamily is a ubiquitous transcription factor with a B3 functional domain in plants, which can respond temporally to abiotic stresses by activating defense compounds and plant hormones. Despite the fact that the B3 genes have been studied in a variety of plants, their role in soybean is still unknown.

Methods

The regulation of melatonin on cold resistance of soybean and the response of B3 genes to cold stress were investigated by measuring biochemical indexes of soybean. Meanwhile, the genome-wide identification of B3 gene family was conducted in soybean, and B3 genes were analyzed based on phylogeny, motifs, gene structure, collinearity, and cis-regulatory elements analysis.

Results

We found that cold stress-induced oxidative stress in soybean by producing excessive reactive oxygen species. However, exogenous melatonin treatment could increase the content of endogenous melatonin and other hormones, including IAA and ABA, and enhance the antioxidative system, such as POD activity, CAT activity, and GSH/GSSG, to scavenge ROS. Furthermore, the present study first revealed that melatonin could alleviate the response of soybean to cold stress by inducing the expression of B3 genes. In addition, we first identified 145 B3 genes in soybean that were unevenly distributed on 20 chromosomes. The B3 gene family was divided into 4 subgroups based on the phylogeny tree constructed with protein sequence and a variety of plant hormones and stress response cis-elements were discovered in the promoter region of the B3 genes, indicating that the B3 genes were involved in several aspects of the soybean stress response. Transcriptome analysis and results of qRT-PCR revealed that most GmB3 genes could be induced by cold, the expression of which was also regulated by melatonin. We also found that B3 genes responded to cold stress in plants by interacting with other transcription factors.

Discussion

We found that melatonin regulates the response of soybean to cold stress by regulating the expression of the transcription factor B3 gene, and we identified 145 B3 genes in soybean. These findings further elucidate the potential role of the B3 gene family in soybean to resist low-temperature stress and provide valuable information for soybean functional genomics study.
Phylogenetic studies of magnoliids: Advances and perspectives
Authors: Shen Zhiguo , Ding Xin , Cheng Jianming , Wu Fangfang , Yin Hengfu , Wang Minyan
Published: January 2023

Magnoliids are the largest flowering plant clades outside of the eudicots and monocots, which are distributed worldwide and have high economic, ornamental and ecological values. Eudicots, monocots and magnoliids are the three major clades of Mesangiospermae, and their phylogenetic relationship is one of the most interesting issues. In recent years, with the continuous accumulation of genomic information, the evolutionary status of magnoliids has become a hot spot in plant phylogenetic research. Although great efforts have been made to study the evolution of magnoliids using molecular data from several representative species such as nuclear genome, plastid genome, mitochondrial genome, and transcriptome, the results of current studies on the phylogenetic status of magnoliids are inconsistent. Here, we systematically describe the current understanding of the molecular research on magnoliid phylogeny and review the differences in the evolutionary state of magnoliids. Understanding the research approaches and limitations of magnoliid phylogeny can guide research strategies to further improve the study of the phylogenetic evolution of magnoliids.
The impact of time spent working from home on affective commitment in the workplace: The mediating role of social relationships and collective aims
Authors: Simon Adél Csenge , Aranyi Gabor , Faragó Klára , Pachner Orsolya Csilla , Kiss Orhidea Edith
Published: January 2023

Introduction

Working from home has become increasingly prevalent due to the COVID-19 pandemic, creating new challenges for organizations and employees. According to the latent deprivation model proposed by Jahoda, work provides latent benefits alongside its material rewards, and losing such benefits leads to a decline in well-being. Organizational affective commitment, or affective commitment within the organization, is a prominent concept in organizational psychology that is linked to lower workforce fluctuation and increased work performance. The present research examined the impact of time spent working from home on affective commitment by examining Jahoda’s “latent functions,” including social contact and collective purpose, representing an innovative application of the latent deprivation model in the context of home office.

Methods

Using an online questionnaire, we collected data from 456 participants (239 female and 217 male) who had been employed for at least 2 years and who had spent a proportion of their time working from home in March and April 2021. The data were analyzed using a path model, in which the potential adverse effect of time spent in home office on affective commitment to the workplace was mitigated by latent functions.

Results

Specifically, we found that more time spent in home office was associated with a decrease in social contact, the impact of which on affective commitment was mediated through the perception of collective purpose.

Discussion

Our findings emphasize the role of the latent benefits of work experienced by employees even when working from home, and the role of those benefits in supporting employees’ commitment to the workplace. We argue that a deeper understanding of such factors is vital, as working from home is expected to remain widespread even after the pandemic.
A longitudinal approach to understanding boredom during pandemics: The predictive roles of trauma and emotion dysregulation
Authors: Bambrah Veerpal , Wyman Amanda , Eastwood John D.
Published: January 2023

Research during the COVID-19 pandemic and prior outbreaks suggest that boredom is linked to poor compliance with critical lifesaving social distancing and quarantine guidelines, as well as to numerous mental health difficulties. As such, continued understanding on what contributes to boredom is imperative. Extending beyond the roles of constraint, monotony, and trait dispositions (e.g., individual differences in boredom propensity), and informed by prior theories on the emotional contributors of boredom, the current longitudinal study examined the predictive role of “pandemic trauma” on people’s boredom, with a focus on how emotion dysregulation mediates this relationship. Community participants (N = 345) completed questionnaires three times across an average of 3 1/2 weeks, rating their pandemic trauma, emotion dysregulation, and boredom over the past week each time. Pandemic trauma was assessed with items querying exposure to coronavirus, as well as the financial, resource-related, and interpersonal pandemic stressors that participants experienced. Emotion dysregulation was assessed with the Difficulties in Emotion Regulation Scale. Boredom was assessed with the short-form Multidimensional State Boredom Scale. The results of a theory-informed mediation model showed that participants’ pandemic trauma at Time 1 positively and modestly predicted their boredom at Time 3 and that this relationship was partially and moderately mediated by participants’ lack of emotional clarity and difficulties with engaging in goal-directed behaviors at Time 2. When people experience pandemic-related trauma, they subsequently struggle to understand their feelings and engage in goal-oriented actions, and, in turn, feel more bored. Theoretical and clinical implications as related to the emotional underpinnings of boredom are discussed.
Situating the KTA gap in clinical research: Foregrounding a discontinuity in practices
Authors: Di Rienzo Giulia
Published: January 2023

In this study, I will claim that we need to rearticulate the so-called “knowledge-to-action” (KTA) gap metaphor in clinical research as a discontinuity of practices. In clinical research, there is a significant delay between the production of research results and their application in policy and practice. These difficulties are normally conceptualized through the metaphor of the KTA gap between scientific knowledge and practical applications. I will advise that it is important to reformulate the terms of the problem, as they suggest the difficulty lies only in the results generated on one side (the laboratory), not reaching the other side (the clinic), and that crossing the gap requires us to simply optimize the transfer and exchange of knowledge. This perspective considers knowledge separate from the practices from which it was generated, making it into a thing that can be transported and transferred largely independently from the communities that produce or “possess” it. The paper then revises the terms of the problem, shifting the focus from knowledge understood as independent from practical circumstances to the situated practices of knowing. Knowledge will then be understood as enacted in practice, emerging as people interact recurrently in the context of established practices. When people coming from different domains and with different “ends-in-view” must coordinate, they have to deal with conceptual and practical tensions, different ways of doing things with their surroundings, and different normative practices. Considering that, the KTA gap will be revised, not as a gap between scientific results and their application in clinical practice, but as a discontinuity in how communities engage with their local contexts and what they perceive as relevant for their activities.
Psychological assessment of parents of people diagnosed with borderline personality disorder and comparison with parents of people without psychological disorders
Authors: Guillén Verónica , Bolo Sara , Fonseca-Baeza Sara , Pérez Sandra , García-Alandete Joaquín , Botella Cristina , Marco José Heliodoro
Published: January 2023

Background

To date, several evidence-based interventions have been created to help relatives of people with Borderline Personality Disorder (BPD), but few studies have analyzed the clinical situation of the family members. The aim of this study was twofold: (1) to explore the clinical symptomatology in a sample of parents of people diagnosed with BPD and compare them with a sample of a sample of people without a relative with a personality disorder, (2) to explore whether the parents of people diagnosed with BPD have psychopathology related to personality disorders (PD) or meet the diagnostic criteria for PD.

Method

Participants were 42 (39.6%) fathers and 64 (60.4%) were mothers and mothers (n = XX, −%) of people diagnosed with BPD, who were selected from a specialized PD unit for treatment. The sample of people without a relative with a PD was obtained from social network announcements. To test for differences between the two groups, Student’s t tests were performed for quantitative variables, and Chi-square tests were performed for categorical variables. Cohen’s d was calculated as a measure of the effect size.

Results

Parents of people with BPD showed greater depressive and anxious symptomatology, higher levels of expressed emotion, and worse quality of life than the sample of people without a relative with a personality disorder. In addition, a high percentage of the parents of people diagnosed with BPD (50%) met the diagnostic criteria for different PD.

Conclusion

Parents of people diagnosed with BPD may need psychological help in various aspects. Therapists are therefore advised to bear in mind the importance of carrying out a psychological assessment of family members and, if necessary, to offer psychological intervention. It is crucial to invite the family to be part of the treatment, since they can be part of the solution.

Clinical Trial registration: ClinicalTrials.gov ID, NCT04160871 (registered November 15, 2019).
Happy Software: An interactive program based on an emotion management model for assertive conflict resolution
Authors: Filella Gemma , Ros-Morente Agnès
Published: January 2023

Emotions are key to conflict resolution: to resolve conflict situations assertively, we must be able to manage the emotions that cause them. After a detailed analysis of the relevant theoretical framework, this paper presents a theoretical emotion management model aimed at assertive conflict resolution. The model, which is described step by step, has been transformed into an interactive program for students, implemented, and assessed in a population aged 8–16 years. The model is divided into four steps or phases. The first is emotional awareness, which consists of understanding and legitimating emotions; the second focuses on reducing emotional intensity and impulsivity; the third encompasses the use of different emotion regulation strategies; and the fourth and final step is assertive communication. Training in this process results in better emotion management, which eventually leads to greater wellbeing and a more positive assessment of new conflicts and aids in the assertive resolution thereof. The final section of the paper summarizes the most important evidence and outcomes of the use of the Happy software so far.
Posner-Schlossman syndrome relapse following inactivated COVID-19 vaccination in China
Authors: Sheng Qilian , Sun Yanan , Zhai Ruyi , Fan Xintong , Ying Yue , Kong Xiangmei
Published: January 2023

Introduction

This retrospective study aims to present the characteristics of Posner-Schlossman syndrome (PSS) relapse following inactivated COVID-19 vaccination.

Methods

From 2020 to 2022, 12 out of 106 PSS patients undergoing relapses after any dose of inactivated COVID-19 vaccines were enrolled. Medical histories, information on the vaccination and systemic adverse events were collected. Patients were treated with corticosteroids, intraocular pressure (IOP)-lowering drugs and systemic immunosuppressive agents (if needed). Daily regimen and release course were noted.

Results

The recurrence rate after vaccination was 11.32% (12/106, 95% CI: 5.29%–17.35%) among 106 PSS patients we surveyed. All the 12 patients were inoculated with inactivated COVID-19 vaccines developed by Sinopharm, China. The mean time of relapse was 5.27 ± 3.72 days (range: 1–13 days, median: 4 days). Higher IOP and more keratic precipitates (KPs) were seen in the relapse following vaccination (33.55 ± 12.99 mmHg, 91.67% had KPs compared to 25.38 ± 3.80 mmHg, 33.33% had KPs in previous relapse, P = 0.009). The mean release course was 30.71 ± 34.74 days for the relapse following vaccination and 7.33 ± 6.51 days for previous relapses. The attack frequency before and after vaccination was 3.56 ± 2.07 and 9.11 ± 7.34 times per year (P = 0.044). Higher daily doses of corticosteroids, IOP-lowering drugs and ganciclovir were needed to maintain stable course, though the difference did not reach statistical significance.

Discussion

More frequent relapses and harder control of IOP were found in PSS relapse following COVID-19 vaccination. Ophthalmologists need to be aware of the group vulnerability and take precautions, though the pathogenesis is still under investigation.
Causal effects and functional mechanisms of the Internet on residents' physical fitness-An empirical analysis based on China family panel survey
Authors: Zhang Long , Lu Chuntian , Yi Cuixia , Liu Zhipeng , Zeng Yuhua
Published: January 2023

Introduction

Physical fitness is an essential part of a healthy lifestyle that concerns the overall health of the nation. Research on the relationship between the Internet and physical fitness has long been caught in the dilemma of “media mobilization” and “media suppression,” and previous studies have rarely examined the causal relationship and functional mechanism.

Methods

This study selected the data of 23,989 samples successfully followed in all three surveys of the China Family Panel Survey (CFPS) from 2014 to 2018 to explore the correlation and causal inference between the Internet and physical fitness by using the Time Fixed Effects Model and cross-lagged models, respectively; meanwhile, the data of 24,687 samples in CFPS 2020 to examine the functional mechanism of the Internet's effect on residents' physical fitness behavior by the KHB method.

Results

We obtained three valuable conclusions as follows: First, there is a significant correlation between the Internet and physical fitness behavior. Second, the Internet use is the cause for the increase in fitness frequency, and there is a rival relationship between Internet duration and fitness time. Third, under regular prevention and control of the COVID-19 epidemic, social capital and health risk perceptions are the functional mechanisms of the Internet influencing fitness behavior, and the mediating effect of psychological health risk perceptions is higher than that of social capital.

Discussion

It's necessary to create an intelligent, informative, and digital sports public service system by enriching and optimizing sports media and facilitating the Internet to serve residents' physical fitness better. The new concept of “Internet plus Fitness” will be of great significance in the implementation of the “Healthy China Initiative.”
Planetary health literacy: A conceptual model
Authors: Jochem Carmen , von Sommoggy Julia , Hornidge Anna-Katharina , Schwienhorst-Stich Eva-Maria , Apfelbacher Christian
Published: January 2023

Education for planetary health could be one of the key levers of the much-needed civilizational turn toward a sustainable and healthy future. Education goes beyond information provision and passing on of knowledge and includes competencies to transfer knowledge from one decision situation to another. There are a range of different literacy concepts from various research perspectives that aim to improve such competencies. While many contain aspects highly relevant for planetary health, there is still no comprehensive and integrative planetary health approach. To fill this research gap, we present a conceptual model of planetary health literacy. By zooming into the model, further details on the necessary core competencies of accessing, understanding, appraising, and applying information in order to make judgements and take decisions regarding planetary health can be found. Zooming out of the model allows a holistic planetary health perspective and shows the potential and opportunities of planetary health literacy for the health of humans and ecosystems. Planetary health literacy encompasses both a life-course and a transgenerational approach, at the individual, societal, and global level. Future educational programs focusing on planetary health could integrate the conceptual model to increase planetary health literacy of individuals, including relevant health literacy agents, and of societies.
ORIGO: A mission concept to challenge planetesimal formation theories
Authors: Marschall Raphael , Thomas Nicolas , Ulamec Stephan , Hviid Stubbe , Mottola Stefano , Vincent Jean-Baptiste , Ferri Francesca , Herique Alain , Plettemeier Dirk , Kereszturi Ákos , Lavagna Michèle R. , Prinetto Jacopo , Dottori Alice , Falke Albert , da Silva Pais Cabral Francisco
Published: January 2023

Comets are generally considered among the most pristine objects in our Solar System. There have thus been significant efforts to understand these bodies. During the past decades, we have seen significant progress in our theoretical understanding of planetesimal/cometesimals (the precursors of comets) formation. Recent space missions—such as ESA’s Rosetta mission to comet 67P/Churyumov-Gerasimenko—have provided observations claimed by proponents of different comet formation theories to validate their scenarios. Yet, no single formation paradigm could be definitively proven. Given the importance of understanding how the first bodies in our Solar System formed, we propose a dedicated mission to address this issue. ORIGO will deliver a lander to the surface of a cometary nucleus where it will characterise the first five m of the subsurface. With remote sensing instruments and the deployment of payload into a borehole, we will be able to study the physico-chemical structure of ancient, unmodified material. The mission has been designed to fit into the ESA M-class mission budget.
Stingless bee keeping: Biocultural conservation and agroecological education
Authors: Aldasoro Maya Elda Miriam , Rodríguez Robles Ulises , Martínez Gutiérrez María Luisa , Chan Mutul Guelmy A. , Avilez López Teresita , Morales Helda , Ferguson Bruce G. , Mérida Rivas Jorge A.
Published: January 2023

Introduction

Stingless bee breeding, also called Meliponiculture, has existed for thousands of years in Mesoamerica among a variety of rural and indigenous cultures. Due to its biocultural importance, it represents a perfect device for agroecological education and scaling, as well as pollinator conservation. At the same time, promoting agroecological transitions are necessary for conserving the cultural heritage that meliponicultures represent.

Methods

We organized a series of activities based on this premise: documenting and dialoguing “saberes contemporáneos” (contemporary knowledges), design and implementation of community agroecological workshops, guided visits at the institutional Meliponary at ECOSUR Villahermosa and promotion of agroecological and biocultural school gardens, all in Tabasco, México. We used “diálogo de saberes” (knowledge dialogues) as a methodological approach, promoting respect for the contribution of the diversity of ontoepistemologies involved. We drew on the ideas of several pedagogues, mainly from popular education and critical pedagogy frameworks.

Results and discussion

We identified characteristics of stingless bees and meliponicultures that make them excellent mediators for biocultural conservation and agroecological education: the complex, deep and beautiful relation between humans and bees; meliponiculture's ecological and cultural importance; stingless bees as pollinators par excellence, landscape connectors, and charismatic species; the association of bees with values around work and community; meliponicultures' symbolic relevance and emotional significance; meliponicultures as promoters of intergenerational dialogue; bee keeping as an activity of caring for the continuity of life; sensory stimulation through contact with colonies; learning through doing in the practice of beekeeping; meliponicultures as an activity requiring skill but no special equipment; and meliponiculture's productive potential. This confluence of teaching-learning opportunities, cultural and moral values, care for the land and biocultural diversity, and economic potential makes meliponiculture a potent catalyst for agroecological learning and transitions.
Use of Portable Devices and an Innovative and Non-Destructive Index for In-Field Monitoring of Olive Fruit Ripeness
Authors: Cinosi Nicola , Portarena Silvia , Almadi Leen , Berrettini Annalisa , Torres Mariela , Pierantozzi Pierluigi , Villa Fabiola , Galletti Andrea , Famiani Franco , Farinelli Daniela
Published: January 2023

A new non-destructive index of absorbance difference (IAD), calculated with different wavelengths as references, near the chlorophyll absorbance peak or that for anthocyanin, has been used to determine the fruit ripening of several fruit species using portable devices such as Standard DA-Meter® and Kiwi-Meter®. In this study, for the first time, the application of the IAD was evaluated on single, intact olives of the Leccino cultivar, as a single non-destructive maturity index to follow ripening in the field, directly on the tree. The IAD determined by both devices was found to be useful for monitoring the olive ripening, however, better performance was achieved with the Kiwi-Meter®. In fact, the IAD determined by this device was more effective at measuring all the studied olive ripening parameters in the calibration and validation datasets with acceptable accuracy. Specifically, better performance of the IDA by Kiwi-Meter® was observed in estimation of the color index (R2 = 0.817, DRMSEC = 0.404 and RPIQ = 3.863 for calibration and R2 = 0.752, DRMSECV = 0.574 and RPIQ = 3.244 for validation), confirming its potential for evaluating the degree of olive ripeness. This novel index has significant applicative possibilities, since it would allow rapid, on-site screening of the best harvesting time to improve the olive production in terms of both yield and quality.
Effects of Different-Sized Cages on the Production Performance, Serum Parameters, and Caecal Microbiota Composition of Laying Hens
Authors: Wan Yi , Du Qiang , Wang Duobiao , Ma Ruiyu , Qi Renrong , Yang Rongbin , Li Xin , Li Junying , Liu Wei , Li Yan , Zhan Kai
Published: January 2023

The effects of four different-sized cages—huge (HC), large (LC), medium (MC), and small (SC) cages—on the productive performance, serum biochemical indices, and caecal microbiota composition of Roman laying hens were investigated. At 44 weeks of age, a total of 450 hens were selected and allocated to the four groups, with six replicates each. Equal stocking density (0.054 m2 per bird) was maintained among the four groups throughout the experiment, and number of birds/cage changed for each treatment. After 2 weeks of preliminary trial, the formal experiment was performed from 46 to 60 weeks of age. The laying rate and feed conversion ratio (FCR) were determined daily, antibody titres were measured every 3 weeks, and serum biochemical parameters and caecal microbiota composition were analysed at 60 weeks of age. Compared to HC and SC, the higher laying rate and lower FCR in MC and LC indicated positive effects on egg production and feed efficiency, while SC showed the highest body weight gain (p < 0.05). With increasing cage size, the serum triglycerides (TG) and total cholesterol (T-CH) levels were reduced, and serum glutathione peroxidase (GSH-Px) activity improved, where birds raised in HCs had the lowest serum TG and T-CH and the highest GSH-Px activity. Twenty-nine different phyla and 301 different genera were detected in the caecal microbiota of birds in the four groups. Methanobrevibacter was significantly higher in the SC than in the other groups (p < 0.05). Faecalibacterium was most abundant in the MC compared with the other groups (p < 0.05) and was significantly positively correlated with serum GSH-Px concentration (R = 0.214, p = 0.0017). Lactobacillus was significantly less abundant in the LC and MC than in the HC and SC groups (p < 0.05) and was significantly positively correlated with body weight (R = 0.350, p = 0.0009) but negatively correlated with laying rate and FCR. In conclusion, MC were superior to HC and LC in improving feed conversion efficiency and caecal microflora composition compared to the SC. An appropriate increase in cage size is beneficial to laying hen production and health.
Detection of Candidate Genes Associated with Fecundity through Genome-Wide Selection Signatures of Katahdin Ewes
Authors: Sánchez-Ramos Reyna , Trujano-Chavez Mitzilin Zuleica , Gallegos-Sánchez Jaime , Becerril-Pérez Carlos Miguel , Cadena-Villegas Said , Cortez-Romero César
Published: January 2023

One of the strategies to genetically improve reproductive traits, despite their low inheritability, has been the identification of candidate genes. Therefore, the objective of this study was to detect candidate genes associated with fecundity through the fixation index (FST) and runs of homozygosity (ROH) of selection signatures in Katahdin ewes. Productive and reproductive records from three years were used and the genotypes (OvineSNP50K) of 48 Katahdin ewes. Two groups of ewes were identified to carry out the genetic comparison: with high fecundity (1.3 ± 0.03) and with low fecundity (1.1 ± 0.06). This study shows for the first time evidence of the influence of the CNOT11, GLUD1, GRID1, MAPK8, and CCL28 genes in the fecundity of Katahdin ewes; in addition, new candidate genes were detected for fecundity that were not reported previously in ewes but that were detected for other species: ANK2 (sow), ARHGAP22 (cow and buffalo cow), GHITM (cow), HERC6 (cow), DPF2 (cow), and TRNAC-GCA (buffalo cow, bull). These new candidate genes in ewes seem to have a high expression in reproduction. Therefore, future studies are needed focused on describing the physiological basis of changes in the reproductive behavior influenced by these genes.
In Vitro Replication of Swine Hepatitis E Virus (HEV): Production of Cell-Adapted Strains
Authors: Ianiro Giovanni , Monini Marina , Ammendolia Maria Grazia , De Sabato Luca , Ostanello Fabio , Vaccari Gabriele , Di Bartolo Ilaria
Published: January 2023

The hepatitis E caused by the virus HEV of genotypes HEV-3 and HEV-4 is a zoonotic foodborne disease spread worldwide. HEV is currently classified into eight different genotypes (HEV-1–8). Genotypes HEV-3 and HEV-4 are zoonotic and are further divided into subtypes. Most of the information on HEV replication remains unknown due to the lack of an efficient cell cultivation system. Over the last couple of years, several protocols for HEV cultivation have been developed on different cell lines; even if they were troublesome, long, and scarcely reproducible, they offered the opportunity to study the replicative cycle of the virus. In the present study, we aimed to obtain a protocol ready to use viral stock in serum free medium that can be used with reduced time of growth and without any purification steps. The employed method allowed isolation and cell adaptation of four swine HEV-3 strains, belonging to three different subtypes. Phylogenetic analyses conducted on partial genome sequences of in vitro isolated strains did not reveal any insertion in the hypervariable region (HVR) of the genomes. A limited number of mutations was acquired in the genome during the virus growth in the partial sequences of Methyltransferase (Met) and ORF2 coding genes.
Impact of Exogenous Xylanase and Phytase, Individually or in Combination, on Performance, Digesta Viscosity and Carcass Characteristics in Broiler Birds Fed Wheat-Based Diets
Authors: Anwar Urooj , Riaz Muhammad , Farooq Khalid Muhammad , Mustafa Riaz , Farooq Umar , Ashraf Muhammad , Munir Hassan , Auon Muhammad , Hussain Mubasher , Hussain Munawar , Ayaz Chisti Muhammad Farhan , Bilal Muhammad Qamar , Rehman Abd ur , Rahman Muhammad Aziz ur
Published: January 2023

The current study was conducted to evaluate the effects of stored wheat-based diet (1.5 and 2.5 years stored wheat) with and without the supplementation of xylanase and phytase enzymes in combination or individually on performance parameters, digestibility, digesta viscosity and carcass characteristics of broilers. For this purpose, a total of 640-day-old male broilers were randomly distributed to the 64 pens, and each pen had 10 birds. Two basal isocaloric and isonitrogenous diets contained 1.5 and 2.5 years stored wheat were formulated in this experiment. In the current study, experimental feeds were prepared by supplementing exogenous enzymes in both basal diets with xylanase (500 XU), phytase (500 FTU) alone or in a combination of phytase and xylanase. Performance parameters data represents that both in starter phase and finisher phase, inclusion of exogenous enzymes xylanase and phytase in both basal diets alone or in combination enhance the feed intake, and body weight gain (p < 0.05) and improve the feed conversion ratio in overall phase (p < 0.05). Similarly, supplementation of exogenous xylanase and phytase alone or in combination enhance the nutrient digestibility and reduce the digesta viscosity (p < 0.05). Based on the results of this experiment, it is concluded that supplementation of exogenous xylanase and phytase enzymes alone or in combination in wheat-based diets (stored wheat 1.5 and 2.5 years) enhance and improves the performance of birds.
Validating a Thoroughbred Racehorse Welfare Index through Horse Behaviour and Trainers’ Reports of Welfare Issues in Their Horses
Authors: Mactaggart Alison Glen , Phillips Clive Julian Christie
Published: January 2023

We validated a Thoroughbred racehorse welfare index, developed initially from expert opinion, by relating it to horse behaviour recorded in a range of training stables and surveying trainers to investigate the environment and management systems for Thoroughbred racehorses. Relationships between the index scores and horse behaviour were observed. Then, an Australia-wide survey of racing industry stakeholders was conducted to identify which parameters are important for welfare in a training stable. Trainers performed well on horsemanship and health/disease aspects. Provisions for ventilation, transportation and nutrition were also scored at high levels. However, provisions for weaning, wastage, assisting horses in coping with heat stress, stabling and the education of racehorses were not as well covered, indicating a need for improvement in these welfare issues. We concluded that our Thoroughbred Racehorse welfare index is able to discriminate between trainers offering differing levels of welfare for the major issues in racehorse training stables.
Resolution Aspects for Near-Field Projections
Authors: Stuckert Alexander , Khanh Tran Quoc
Published: January 2023

This work intends to define the resolution requirements for near-field projections in a psycho-physical study design to evaluate the participants’ perception under the influence of different ambient lighting levels and various viewing distances. The variation in ambient lighting and viewing distances relates to various daytime and critical distances in urban environments. The application of near-field projections increases the popularity of communication- or safety-relevant projections, such as for automated vehicles. However, previous studies in the filming industry have shown that the resolution requirements differ depending on the application. In this work, a field study design presents an experimental approach to define a perceived resolution on the street surface in the near field around the vehicle. Furthermore, the study evaluates the influence of viewing distance, ambient lighting and projection content on the perceived resolution in detail. The results reveal a significant dependency on ambient lighting (p < 0.05). Furthermore, this work states that the symbol-based projection has lower resolution requirements, e.g., a viewing distance of 1 m and 3 m results in a 2 pixels per degree resolution compared to the text-based projection in the parking garage scenario. Nevertheless, in the dusk/dawn scenario, the perceived resolution can be grouped for viewing distances above 1 m for content-independent projections.