bluuebunny/crossref_metadata_2025 huggingface dataset:
TwoStepCisMR: A Novel Method and R Package for Attenuating Bias in cis-Mendelian Randomization Analyses
Authors: Woolf Benjamin , Zagkos Loukas , Gill Dipender
Published: August 2022

Mendelian randomisation (MR) is an increasingly popular method for strengthening causal inference in epidemiological studies. cis-MR in particular uses genetic variants in the gene region of a drug target protein as an instrumental variable to provide quasi-experimental evidence for on-target drug effects. A limitation of this framework is when the genetic variant is correlated to another variant that also effects the outcome of interest (confounding through linkage disequilibrium). Methods for correcting this bias, such as multivariable MR, struggle in a cis setting because of the high correlation among genetic variants. Here, through simulation experiments and an applied example considering the effect of interleukin 6 receptor signaling on coronary artery disease risk, we present an alternative method for attenuating bias that does not suffer from this problem. As our method uses both MR and the product and difference method for mediation analysis, our proposal inherits all assumptions of these methods. We have additionally developed an R package, TwoStepCisMR, to facilitate the implementation of the method.
Integrative Investigation of Root-Related mRNAs, lncRNAs and circRNAs of “Muscat Hamburg” (Vitis vinifera L.) Grapevine in Response to Root Restriction through Transcriptomic Analyses
Authors: Liu Jingjing , Li Hui , Zhang Lipeng , Song Yue , He Juan , Xu Wenping , Ma Chao , Ren Yi , Liu Huaifeng
Published: August 2022

Root restriction is a physical and ecological cultivation mode which restricts plant roots into a limited container to regulate vegetative and reproduction growth by reshaping root architecture. However, little is known about related molecular mechanisms. To uncover the root-related regulatory network of endogenous RNAs under root restriction cultivation (referred to RR), transcriptome-wide analyses of mRNAs, long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) involved in root development were performed. During root development, RR treatment had a positive effect on root weight, typically, young roots were significantly higher than conventional cultivation (referred to NR) treatment, suggesting that root architecture reconstruction under RR was attributed to the vigorous induction into lateral roots. Furthermore, a total of 26,588 mRNAs, 1971 lncRNAs, and 2615 circRNAs were identified in root of annual “Muscat Hamburg” grapevine by the transcriptomic analyses. The expression profile of mRNAs, lncRNAs and circRNA were further confirmed by the quantitative real-time PCR (RT-qPCR). Gene ontology enrichment analysis showed that a majority of the differentially expressed mRNAs, lncRNAs and circRNAs were enriched into the categories of cellular process, metabolic process, cell part, binding, and catalytic activity. In addition, the regulatory network of endogenous RNAs was then constructed by the prediction of lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA network, implying that these RNAs play significant regulatory roles for root architecture shaping in response to root restriction. Our results, for the first time, the regulatory network of competitive endogenous RNAs (ceRNAs) functions of lncRNA and circRNA was integrated, and a basis for studying the potential functions of non-coding RNAs (ncRNAs) during root development of grapevine was provided.
CRISPR-Guided Proximity Labeling of RNA–Protein Interactions
Authors: Lu Mingxing , Wang Zuowei , Wang Yixiu , Ren Bingbing
Published: August 2022

Proximity labeling employs modified biotin ligases or peroxidases that produce reactive radicals to covalently label proximate proteins with biotin in living cells. The resulting biotinylated proteins can then be isolated and identified. A combination of programmable DNA targeting and proximity labeling that maps proteomic landscape at DNA elements with dCas9-APEX2 has been established in living cells. However, defining interactome at RNA elements has lagged behind. In combination with RNA-targeting CRISPR-Cas13, proximity labeling can also be used to identify proteins that interact with specific RNA elements in living cells. From this viewpoint, we briefly summarize the latest advances in CRISPR-guided proximity labeling in studying RNA–protein interactions, and we propose applying the most recent engineered proximity-labeling enzymes to study RNA-centric interactions in the future.
Characterization of Autoimmune Thyroid Disease in a Cohort of 73 Paediatric Patients Affected by 22q11.2 Deletion Syndrome: Longitudinal Single-Centre Study
Authors: Ricci Silvia , Sarli Walter Maria , Lodi Lorenzo , Canessa Clementina , Lippi Francesca , Azzari Chiara , Stagi Stefano
Published: August 2022

Background. Chromosome 22q11.2 Deletion Syndrome (22q11.2DS) is the most frequent microdeletion syndrome and is mainly characterized by congenital cardiac defects, dysmorphic features, hypocalcemia, palatal dysfunction, developmental delay, and impaired immune function due to thymic hypoplasia or aplasia. Thyroid anomalies are frequently reported in patients with 22q11.2DS, although only a few well-structured longitudinal studies about autoimmune thyroid disease (ATD) have been reported. Aim. To longitudinally evaluate the frequency of thyroid anomalies and ATD in patients with 22q11.2DS. Patients and Methods. Pediatric patients with a confirmed genetic diagnosis of 22q11.2DS were recruited and followed up on longitudinally. Clinical, biochemical, and immunological data were collected, as well as thyroid function, autoimmunity, and thyroid sonographic data. Results. The study included 73 children with 22q11.2DS, with a mean follow-up duration of 9.51 ± 5.72 years. In all, 16 of the 73 enrolled patients (21.9%) developed ATD before 18 years of age (mean age 12.92 ± 3.66 years). A total of 20.5% developed Hashimoto’s Thyroiditis (HT), of whom 50% required L-thyroxine treatment; 1.4% developed Graves Disease. Thyroid hypoplasia was found in 6/16 patients with ATD and left lobe hypoplasia in 9/16 patients. These features were also found in patients affected by 22q11.2DS without ATD. Among patients who developed ATD, at the first altered ultrasound scan, the most frequent anomalies suggestive of thyroiditis were inhomogeneous echotexture, diffuse or irregular hypo-echogenicity, and vascular overflow. Conclusion. We strongly recommend periodic screening of thyroid function and for autoimmunity in patients affected by 22q11.2DS. Along with blood tests, ultrasound scans of the thyroid gland should be performed periodically since some patients who go on to develop an ATD could have specific anomalies on ultrasound prior to any other anomaly.
Recycling and Reuse of Mine Tailings: A Review of Advancements and Their Implications
Authors: Araujo Francisco , Taborda-Llano Isabella , Nunes Everton , Santos Rafael
Published: August 2022

Mining is an important industry, accounting for 6.9% of global GDP. However, global development promotes accelerated demand, resulting in the accumulation of hazardous waste in land, sea, and air environments. It reached 7 billion tonnes of mine tailings generated yearly worldwide, and 19 billion solid tailings will be accumulated by 2025. Adding to this, the legacy of environmental damage from abandoned mines is worrying; there are around 10,000 abandoned mines in Canada, 50,000 in Australia, and 6000 in South Africa, as well as 9500 coal mines in China, reaching 15,000 by 2050. In this scenario, restoration techniques from mining tailings have become increasingly discussed among scholars due to their potential to offer benefits towards reducing tailing levels, thereby reducing environmental pressure for the correct management and adding value to previously discarded waste. This review paper explores the available literature on the main techniques of mining tailing recycling and reuse and discusses leading technologies, including the benefits and limitations, as well as emerging prospects. The findings of this review serve as a supporting reference for decision makers concerning the related sustainability issues associated with mining, mineral processing, and solid waste management.
The Metaphysics of Sophistry: Protagoras, Nāgārjuna, Antilogos
Authors: Reames Robin
Published: August 2022

There is no category of thought more deliberately or explicitly relegated to a subordinate role in Plato’s dialogues than Sophists and sophistry. It is due to Plato’s influence that terms “sophist” and “sophistry” handed down to us have unilaterally negative associations—synonymous with lies and deception, obscurantism and false reasoning. There are several reasons to be dubious of this standard view of the Sophists and their practices. The primary reason addressed in this essay is that the surviving fragments of the Sophists do not accord with this standard view, a discrepancy that is particularly acute in the case of the 5th-century sophist Protagoras. This essay attends to Protagoras’s doctrines concerning antilogos, the sophistic practice of contradiction and negation. I contend that sophistic antilogos was a paradoxical practice that embodied metaphysical stakes for language and discourse. I challenge the standard view of Sophists and their antilogos by reconstructing a speculative counter-definition: a method for instantiating through language an ontology of flux and becoming over and against what would come to be a Platonist metaphysics of enduring, pure Being. I do this through a comparative analysis of Protagoras and the second century C.E. South Asian Buddhist thinker, Nāgārjuna.
A Study on CKD Progression and Health Disparities Using System Dynamics Modeling
Authors: Yinusa Ahmeed , Faezipour Misagh , Faezipour Miad
Published: August 2022

Chronic kidney disease (CKD) is one of the most prevalent national health problems in the United States. According to the Center for Disease Control and Prevention (CDC), as of 2019, 37 million of the US’s adult population have been estimated to have CKD. In this respect, health disparities are major national concerns regarding the treatments for patients with CKD nationwide. The disparities observed in the healthcare interventions for patients with this disease usually indicate some significant healthcare gaps in the national public health system. However, there is a need for immediate intervention to improve the present healthcare conditions of minorities experiencing CKD nationwide. In this research, the application of system dynamics modeling is proposed to model the CKD progression and health disparities. This process is based on the health interventions administered to minorities experiencing CKD. The graphical results from the model show that there are relationships among the dynamic factors influencing the incidence and prevalence of CKD. Hence, healthcare disparities are inherent challenges in the treatment and management of this disease.
Nurses’ Opinions on Frailty
Authors: Gobbens Robbert J. , Vermeiren Sofie , Van Hoof An , van der Ploeg Tjeerd
Published: August 2022

Nurses come into frequent contact with frail older people in all healthcare settings. However, few studies have specifically asked nurses about their views on frailty. The main aim of this study was to explore the opinions of nurses working with older people on the concept of frailty, regardless of the care setting. In addition, the associations between the background characteristics of nurses and their opinions about frailty were examined. In 2021, members of professional association of nurses and nursing assistants in the Netherlands (V&VN) received a digital questionnaire asking their opinions on frailty, and 251 individuals completed the questionnaire (response rate of 32.1%). The questionnaire contained seven topics: keywords of frailty, frailty domains, causes of frailty, consequences of frailty, reversing frailty, the prevention of frailty, and addressing frailty. Regarding frailty, nurses especially thought of physical deterioration and dementia. However, other domains of human functioning, such as the social and psychological domains, were often mentioned, pointing to a holistic approach to frailty. It also appears that nurses can identify many causes and consequences of frailty. They see opportunities to reverse frailty and an important role for themselves in this process.
Adherence to Antibiotic Prescription of Dental Patients: The Other Side of the Antimicrobial Resistance
Authors: D’Ambrosio Francesco , Di Spirito Federica , De Caro Francesco , Lanza Antonio , Passarella Daniela , Sbordone Ludovico
Published: August 2022

Since non-adherence to antibiotic therapy can cause several problems, including antimicrobial resistance (AMR) and treatment failures, the present study evaluated adherence to oral antibiotic therapy and AMR awareness among consecutively enrolled dental patients. Data concerning age, gender, socioeconomic status, education level, cohabitation, and general health were retrieved from medical records. AMR awareness was investigated through direct questions and adherence to antibiotic treatment was assessed through a modified Italian version of the Morisky medical scale-8 items. Participants’ characteristics were analyzed in relation to treatment adherence and AMR, using a Χ2 independence test (significance level of α <0.1). Dental patients generally showed a low (51.82%) adherence to oral antibiotic therapy, and medium and high adherence was reported only by 29.37% and 18.81% of participants. Treatment adherence was similar in relation to participants’ gender and age but significantly lower in subjects with only secondary school graduation and higher in participants with higher education levels. Non-cohabitants were significantly more adherent than cohabitants. AMR awareness was declared by 42.15% of males and 38.70% of females: 56.52% of dental patients aware of AMR were 18–38 years old, 35.20% were 39–59 years old, and 26.95% were aged between 60 and 80. Further studies are needed to develop adequate strategies, expanding dental patients’ knowledge of AMR, thus optimizing the benefits and reducing the risks of antibiotic administration in dental patients.
Musical Breaks—Live Music in a Hemodialysis Setting—A Qualitative Study on Patient, Nurse, and Musician Perspectives
Authors: Bro Margrethe Langer , Finderup Jeanette , Smilde Rineke , Gram Bibi , Dreyer Pia
Published: August 2022

The aim of this hermeneutic-phenomenological study was to explore the perspectives of 12 patients, 17 nurses, and 4 musicians on patient-tailored live music interventions in a hemodialysis setting. Twenty-six semi-structured interviews were collected—17 with patients, 9 with nurses. Furthermore, 18 moderate participation observations, whilst 1 semi-structured group interview with 3 nurses and 3 musicians, and 13 reflective journals from musicians were collected. Within the analysis—based on Ricoeur’s theory of interpretation—two overall themes emerged: (1) the inner space and (2) the participating space, followed by five subthemes: (1a) Entering a calm and enjoyable pause bubble; (1b) Resting in a thought-free state of mind; (1c) Traveling in the past and catching the moment through heartfelt music; (2a) Bringing positive changes into life; (2b) The artistic quality mediating a magnificent and beautiful experience. We found that patient-tailored live music was a meaningful break, influencing mental and physical well-being, time perception, community, work environment, and artistic approach. The artistic quality of the music was essential—together with the musicians’ social awareness, empathy, and ability to interact with the patients—in creating meaningful moments for patients and staff. Overall, the music interventions were a welcome change in a predictable world of stressful routines and repetitive treatments.
A Simulation-Based Clinical Nursing Education Framework for a Low-Resource Setting: A Multimethod Study
Authors: Salifu David Abdulai , Heymans Yolande , Christmals Christmal Dela
Published: August 2022

Simulation-based clinical education is a useful strategy for teaching, learning, and assessing clinical competence in health professions education. However, the use of simulation-based clinical nursing education (SBCNE) in low-resource settings such as Ghana has been hampered by the lack of a context-specific framework to guide its design, implementation, and evaluation. This study sought to develop a context-specific framework to guide the design, implementation, and evaluation of SBCNE in a low-resource setting. The study employed a sequential multimethod design, comprising a scoping review; qualitative descriptive design (situational analysis) made up of two parts–focus group discussions (FGDs) with post-registration nurses and nursing students, and semi-structured interviews with nurse educators; and narrative synthesis of the scoping review and situational analysis data, used to develop a draft SBCNE framework for a low-resource setting. The draft SBCNE framework was evaluated by stakeholders of nursing education and practice using nominal group discussions. The framework is comprised of five constructs (context, planning, design, community of learning, and outcomes). The user-centric, comprehensive, context-specific SBCNE framework has the potential to enhance the implementation of simulation in nursing education and the development of clinical competence in a low-resource setting. As a result, we urge nursing leaders and nurse educator unions to take the lead in lobbying regulatory bodies, the central government, and their development partners to provide the necessary financial support and resources for the implementation of the framework and adoption of SBCNE in low-resource settings.
Behavioral Strengths and Difficulties and Their Associations with Academic Performance in Math among Rural Youth in China
Authors: Yu Wenjing , Abbey Cody , Qian Yiwei , Wang Huan , Rozelle Scott , Singh Manpreet K.
Published: August 2022

Behavioral strengths and difficulties among children and adolescents may be significantly associated with their academic performance; however, the evidence on this issue for rural youth in developing contexts is limited. This study explored the prevalence and correlates of mental health from three specific dimensions—internalizing problems, externalizing problems, and prosocial behavior—measured by the Strengths and Difficulties Questionnaire (SDQ), and the association of these dimensions with academic performance in math among a sample of 1500 students in rural China. Our findings indicated that students in rural China had worse behavioral difficulties and poorer prosocial skills when compared to most past studies conducted inside and outside of China. In addition, total difficulties and prosocial scores on the SDQ were significantly associated with student math test scores, as students whose externalizing, internalizing, and prosocial scores were in the abnormal range scored lower in math by 0.35 SD, 0.23 SD, and 0.33 SD, respectively. The results add to the growing body of empirical evidence related to the links between social environment, mental health, and academic performance in developing countries, highlighting the importance of students’ mental health for their academic performance, and of understanding risk factors in the social environment among rural youth in developing countries.
Sustainable Management of Green Mold Disease of White Button Mushroom Using Botanicals and Biocontrol Agents under Temperate Conditions
Authors: Altaf Suhail , Jan Shaheen Kousar , Basu Umer , Ahanger Shafat Ahmad , Dave Anand , Kakraliya Sardar Singh , Baazeem Alaa , Mishra Ajay Kumar , Kumar Anupam , Shah Immad Ahmad , Mushtaq Muntazir
Published: August 2022

Green mold (caused by Trichoderma harzianum) is a destructive disease in mushrooms which limits commercial production. The present investigation was carried out to verify the in vitro and in vivo effect of locally available botanicals and bacterial biocontrol agents against this disease. The in vitro evaluation of ethanol extract of botanicals against mycelial growth of T. harzianum at 1, 2, and 3% concentrations showed that Juglans regia and Allium sativum exhibited maximum mycelial growth inhibition of 84.9 and 79.8%, respectively. When the same botanicals were tested against the mycelial growth of A. bisporus, it was observed that J. regia, Curcuma longa, and Azadirachta mellea were least inhibitory (4.66–7.4%). From the in vivo evaluation of plant botanicals at 2% concentration, J. regia and C. longa had the highest average weight (11.8–11.9 g) of a single fruit body and a combined button yield of 11.3–11.9 kg/quintal compost. Among the bacterial bioagents evaluated in vitro, Pseudomonas flourescens, Azotobacter sp., and Bacillus subtilis displayed stimulatory effects of varying degrees on the mycelial growth of A. bisporus but exhibited antagonistic effects on T. harzianum. B. subtilis-38, and P. flourescens-104. Azotobacter-108 caused the highest mycelial growth inhibition of 97.6, 97.4, and 90.3% of T. harzianum, respectively. The current study reveals that the integration of botanical and bacterial antagonists in pathogen-infested white button mushroom casing reduces green mold infection with corresponding yield gains.
Application of Rosemary and Eucalyptus Essential Oils on the Preservation of Cucumber Fruit
Authors: Xylia Panayiota , Chrysargyris Antonios , Shahwar Durray , Ahmed Zienab F. R. , Tzortzakis Nikolaos
Published: August 2022

Cucumber (Cucumis sativus L.) is one of the most significant and frequently produced vegetable crops. However, because of its high perishability, keeping its quality from the farm to the market is a challenging problem. Various techniques have been employed to reduce postharvest losses and to extend the shelf life of vegetables and fruits. Recently, another approach is the application of eco-friendly elicitors as essential oils-EOs to prevent fruit decay and enhance its quality. Therefore, the purpose of this study was to assess the effectiveness of an environmentally friendly product (EP) based on rosemary and eucalyptus essential oils and two distinct application techniques (vapor and dipping) on maintaining quality characteristics of cucumber fruits during storage at 11 °C and ~90% relative humidity (RH) for two weeks. The results showed that the EP was successful in preserving cucumber fruit quality. Vaporized cucumbers did not show any significant weight losses, whereas dipped cucumbers showed a greater weight loss at 0.4% EP (3.5%) compared with the control (3%). Fruit treated with EP (vapor or dipping) or chlorine maintained firmness and ripening index during storage. Total soluble solids (TSS) levels were similar in all vapor-treated fruit while dipping application of chlorine increased TSS (4 oBrix) after 7 days of storage. Vapor or dipping application of EP or chlorine had a slight effect on cucumber color. Vaporized (EP or chlorine) cucumbers had comparable total phenols and antioxidant activity when compared with the control treatment, while dipped fruit had lower total phenols content and antioxidant activity at 0.8% EP after 7 days of storage. These findings indicate that the evaluated EP (vapor or dipping) can be a potential natural alternative to be used to preserve fresh produce instead of the common sanitizing agent (chlorine). Nevertheless, the application method and conditions should be further optimized for every product.
Home Gardening and Food Security Concerns during the COVID-19 Pandemic
Authors: Turnšek Maja , Gangenes Skar Siv-Lene , Piirman Marit , Thorarinsdottir Ragnheiður I. , Bavec Martina , Junge Ranka
Published: August 2022

At times of crisis, home gardening has often been sought out as a potential solution for threats to food security and as a measure to increase socio-psychological effects, such as public sense of self-efficacy, trust in the government and care for one’s wellbeing. The objective of this study was to investigate if home gardening increased during the COVID-19 pandemic in the spring/summer of 2020 and to provide socio-psychological insights into the explanatory factors of such an increase. An explanatory theoretical model of home gardening was proposed and tested to analyse whether home gardening is correlated to food security concerns, and if so, to what extent. A non-representative survey was conducted in five European countries (Slovenia, Norway, Estonia, Switzerland, and Iceland) using snowball sampling via social media networks, reaching 1144 participants. The results showed the pandemic did prove to be an important psychological push towards home gardening prompted by food security concerns. Measured as loose as introducing at least one new gardening activity during COVID-19, this study found an approximately 10% increase in home gardening during the first wave of COVID-19 in the sample population, which was skewed towards educated, female, middle-class Europeans.
Seasonal Change in Phytochemical Composition and Biological Activities of Carissa macrocarpa (Eckl.) A. DC. Leaf Extract
Authors: Ramasar Reshika , Naidoo Yougasphree , Dewir Yaser Hassan , El-Banna Antar Nasr
Published: August 2022

Genus Carissa represents several species that are reported to be of great phyto-medicinal and ethnopharmacological value. However, Carissa macrocarpa is relatively understudied. Furthermore, environmental conditions such as seasonal changes are known to affect the phytochemical composition of medicinal plants. Therefore, this study aimed to investigate the phytochemical composition and biological activity of the leaf extracts of C. macrocarpa in the summer and winter seasons. The phytochemical screening of C. macrocarpa leaves showed positive results for a variety of phytochemicals, such as alkaloids, tannins, phenols, naphthoquinones, flavonoids, saponins, steroids, proteins, carbohydrates, mucilage, gum and resin. The methanolic extract was evaluated for its antibacterial activity against Escherichia coli and Staphylococcus aureus using the agar well diffusion method. The winter leaf extract was distinguished for its potential antibacterial activity against both bacterial strains with inhibition zones (mm) of 8.17 ± 1.04 and 6.83 ± 0.58 at 10 mg/mL. The antioxidant activity of the leaf extracts was evaluated using the 2, 2′-diphenyl-1-picrylhydrazyl (DPPH) assay. The percentage scavenging activities of the different extracts were significantly greater than that of the control. Furthermore, at 15, 30, 60, 120 and 240 µg/mL, the percentage scavenging activities of the winter methanol leaf extract were 74.65, 78.31, 85.45, 90.02 and 95.68%, and those of the summer one were 71.66, 73.57, 84.05, 88.22 and 96.28%, respectively, indicating that the methanol leaf extract had greater percentage scavenging activity in winter than in summer. In winter, the IC50 value of the methanol leaf extract (0.67 µg/mL) was lower than that of ascorbic acid (8.26 µg/mL). It is concluded that winter is the optimal season to harvest leaves of C. macrocarpa for medicinal use. To the best of our knowledge, this is the first report that relates the phytochemical composition and medicinal properties of C. macrocarpa to changes in seasons. The results obtained are promising, and this species should be further explored to decipher its pharmacological worth.
Ethylene and Chitosan Affected the Seed Yield Components of Onion Depending More on the Dose than Timing of Application
Authors: Vecchiotti Daniele , Angeletti Francesco G. S. , Romanazzi Gianfranco , Mariotti Marco , Saia Sergio
Published: August 2022

(1) Background: the production of onion seeds is limited by the competition between seeds and the vegetative organs and by scape lodging. However, information on the effects of plant growth regulation on onion seed production is scarce. Aim of the present study was to evaluate the seed yield components and germination ability of onion seeds as affected by the timing and dose of an ethylene application, a plant growth regulator able to modulate shoot–flower competition; and chitosan, an elicitor of plant defense mechanisms able to increase its tolerance to various stresses. (2) Methods: Onion was treated with ethylene at the recommended dose (100% RD) of a commercial product, at 150% RD in two contrasting phenological phases or untreated (control), or ‘with’ or ‘without’ chitosan, and the seed yield components and germination trend were measured. (3) Results: 100% RD at an early phase of growth did not influence the seed yield and increased the thousand seed weight (TSW) by 3.2%. The application of 150% RD decreased the seed yield by 33.5%, and this occurred irrespective of the timing of application. Such decreases were due to a reduction in the number of seeds per flower. The application of chitosan did not affect the crop at 100% RD and increased the seed yield and slightly increased, but not significantly, the TSW under 150% RD. Germination of the fresh seed was 92%, and 17 months of aging reduced it by 14%, with no effects of the treatments on the germination pattern. (4) Conclusions: the ethylene application mostly affected TSW but not the yield, whereas high doses of ethylene reduced yields irrespective of the timing of application. Such a result may have been due to a delay in the flowering onset that occurred in a relatively dry month. Chitosan sustained its yield when the yield potential was reduced by 150% RD, and such a result was likely due to physical protection from the transpiration since the synthetic fungicides applied did not likely allow the pathogens to infections. These results have implications for establishing the timing and dose of application of plant growth regulators and elicitors in seed onions to sustain the seed quality.
Combination of Selenium and Methyl Jasmonate Controls Postharvest Tomato Gray Mold by Damaging the Membrane System
Authors: Yuan Xiang , Li Changyin , Xie Jiatao , Li Keyi , Chen Siqi , Yuan Linxi , Hu Chengxiao , Wang Xu , Zhao Xiaohu
Published: August 2022

Tomato, which is regarded as an important worldwide crop, is susceptible to gray mold caused by Botrytis cinerea. Selenium and methyl jasmonate can act as antifungal agents against pathogenic infections. To clarify the effect of selenium and methyl jasmonate on the fungal pathogen, the spore germination and mycelial growth of B. cinerea were investigated in vitro using the growth rate method. Additionally, the electrical conductivity, soluble protein content, malondialdehyde content and oxalic acid secretion of B. cinerea mycelium were also determined to further explore the antifungal mechanism of selenium and methyl jasmonate. The results showed that selenium application significantly increased cell membrane permeability and malondialdehyde content, and methyl jasmonate treatment decreased the soluble protein content in mycelium of B. cinerea. Furthermore, supplementation of the medium with both selenium and methyl jasmonate effectively inhibited spore germination and colony growth of B. cinerea by compromising membrane integrity, and significantly reduced soluble protein content and the oxalic acid secretion of hypha. The resulting incidence of postharvest tomato gray mold with the combination of selenium and methyl jasmonate was 34.7%, which was approximately half of that of the control. To sum up, the combined use of selenium and methyl jasmonate inhibited the normal physiological activity and pathogenicity of B. cinerea, which suggests that selenium and methyl jasmonate have the potential for controlling gray mold disease caused by B. cinerea in postharvest fruits and vegetables. These findings may offer a promising and eco-friendly strategy to control gray mold disease in postharvest fruits and vegetables.
Why They Stayed and Why They Left—A Case Study from Ellicott City, MD after Flash Flooding
Authors: Chan Alisha Yee , Burrows Kate , Bell Michelle L.
Published: August 2022

Ellicott City, MD was devasted by flash flooding in 2016 and 2018. A lack of qualitative research has been conducted on topics related to sense of place and flash flooding, especially in the United States. In this study, we reveal reasons why some who experienced flash flooding continued to stay the flood zone and why some leave. We utilized a phenomenological approach to answer these research questions. Data were generated through in-depth interviews with 19 participants from the Historic District and adjacent neighborhoods in Ellicott City. The most common reasons participants stayed were: (1) Community Impact, (2) Historical Land, and (3) Financial Burden. The most common reasons participants left were: (1) Emotional Exhaustion and Frustration, (2) Fear/Anxiety, and (3) Financial Burden. The results of our study indicate that reasons individuals who experience flash flooding stay, or leave may include community/historical, environmental, emotional, and economic factors. This reveals the complexity of relocation and sense of place after natural/environmental disasters and supports previous literature that suggests tailored response efforts based on these unique set of burdens. This paper aims to identify burdens and understand flood victims’ decisions to help policy makers improve flood response efforts.
Community Stakeholders’ Perceptions on Barriers and Facilitators to Food Security of Families with Children under Three Years before and during COVID-19
Authors: Varela Elder Garcia , Zeldman Jamie , Mobley Amy R.
Published: August 2022

Children living in food-insecure households have poorer overall health than children in food-secure households. While U.S. nutrition assistance programs provide resources, these cannot consistently offer age-appropriate nutritional foods for young children. This study aimed to determine community stakeholders’ perceptions of the barriers and facilitators to obtaining adequate, high-quality, and age-appropriate foods for children ages 0–3 in Florida before and during COVID-19. Community stakeholders (n = 32) participated in a 60 min interview via Zoom using a semi-structured script based on the PRECEDE component of the PRECEDE–PROCEED model. Interviews were transcribed verbatim and coded by two researchers using a thematic analysis approach. Stakeholders’ perceptions revealed a lack of awareness surrounding eligibility for assistance programs, a lack of knowledge regarding how to obtain resources and services, and stigma associated with receiving benefits. These remained significant barriers to obtaining healthful foods for households with young children before and during COVID-19. Nonetheless, barriers were exacerbated during the pandemic. Unemployment rates rose, intensifying these households’ financial hardships and food insecurity levels. Likewise, stakeholders suggested the need for families to become more aware of federal assistance eligibility requirements and available opportunities via social media and referrals. Identifying risk factors associated with food insecurity can inform future interventions to safeguard young children’s health and well-being.
Urban Shrinkage and Urban Vitality Correlation Research in the Three Northeastern Provinces of China
Authors: Jiang Yihao , Chen Zhaojin , Sun Pingjun
Published: August 2022

In the global trend of urban shrinkage, urban vitality, as one of the important representations of high-quality urban development, has become a breakthrough. More and more scholars advocate to awaken urban vitality, so as to realize the high-quality development of shrinking cities. This paper takes the municipal districts of 34 cities in the three northeastern provinces of China as study areas, based on the broad concept of urban shrinkage, selects the indicators of population, economy and society, and uses the “two-step diagnostic method” which is consistent with Chinese conditions to identify the urban shrinkage from 2010 to 2018. In this research, the indexes of economic, social, cultural, environmental and spatial dimensions are selected, and the urban vitality and the vitality of each dimension from 2010 to 2018 are calculated and analyzed by using the entropy weight method (EWM). Then, this paper analyzes the correlation between urban shrinkage and urban vitality by Pearson correlation coefficient. The results show that: (1) urban shrinkage in the three northeastern provinces of China has become a regional remarkable phenomenon, which is also an inevitable process in some regions of China and even the world; (2) overall, the urban vitality of cities in the three northeastern provinces of China is steady and rising a little, and there is an obvious spatial agglomeration pattern like “central city polarization”; (3) there is a significant correlation between urban shrinkage and urban vitality, that is, the lower the degree of urban shrinkage, the higher the urban vitality, showing the opposite trend in the process of urban development; (4) the influence of urban shrinkage on each dimension of urban vitality is different, and the correlation results are different, too. In the planning process of shrinking cities in the future, paying attention to the relationship between urban vitality and urban shrinkage, conducting benign guidance on this basis, and adjusting urban vitality elements of different dimensions to stimulate urban development power can enhance urban competitiveness and achieve better development.
Factors Related to Stunting Incidence in Toddlers with Working Mothers in Indonesia
Authors: Laksono Agung Dwi , Sukoco Noor Edi Widya , Rachmawati Tety , Wulandari Ratna Dwi
Published: August 2022

Previous studies have suggested that a toddler stunting is closely related to maternal characteristics. Working mothers, as a group, are vulnerable to having a stunted toddler. The present research aimed to analyze factors related to stunting incidence in toddlers with working mothers in Indonesia. The study sampled 44,071 toddlers with working mothers. The final stage used a multinomial logistic regression test. The study found that working mothers living in rural areas have a higher probability of having stunted or severely stunted toddlers. Maternal age partially affects the incidence of stunted toddlers in Indonesia. Mothers in the ≤19 age group are 1.461 (95% CI 1.140–1.872) times more likely than those in the ≥45 group to have a severely stunted toddler. Those who were never married were 1.433 (95% CI 1.006–2.043) times more likely than those who were divorced/widowed to have a severely stunted toddler. A married mother was 0.734 (95% CI 0.617–0.872) times less likely to have a severely stunted toddler than a divorced/widowed mother. Better education is protective against working mothers having stunted toddlers. Moreover, the present study found that the toddler’s age determined the incidence of stunted toddlers. This study concluded that there are five variables related to stunting incidence in toddlers with working mothers in Indonesia: residence, age, marital status, education, and toddler age.
Risk Factors for Problematic Drinking in One’s Thirties and Forties: A Longitudinal Analysis of the 1970 British Cohort Study
Authors: O’Donovan Gary , Hamer Mark
Published: August 2022

Alcohol drinking and risk factors for problematic drinking may vary across a lifespan. The objective of this study was to identify risk factors for problematic drinking in men and women in their thirties and forties. Alcohol drinking and potential risk factors for problematic drinking were assessed at ages 30, 34, 42, and 46 in the 1970 British Cohort Study. Multilevel models included 10,079 observations in 3880 men and 9241 observations in 3716 women. In men, formerly smoking, currently smoking, having a degree, having malaise, and having a mother who drank while pregnant were independently associated with increased risk of problematic drinking. In women, formerly smoking, currently smoking, being physically active in one’s leisure time, having a degree, having a managerial or professional occupation, having malaise, and having a mother who drank while pregnant were independently associated with increased risk of problematic drinking. In men and women, cohabiting as a couple was associated with decreased risk of problematic drinking. This study suggests that several risk factors may be associated with problematic drinking in men and women in their thirties and forties. Policy makers should consider the role of modifiable risk factors in the prevention of problematic drinking.
Association between Child Abuse and Poor Oral Habits in Mongolian Adolescents
Authors: Okawara Aina , Matsuyama Yusuke , Yoshizawa Araki Miyu , Unnai Yasuda Yuko , Ogawa Takuya , Tumurkhuu Tsasan , Ganburged Ganjargal , Bazar Amarsaikhan , Fujiwara Takeo , Moriyama Keiji
Published: August 2022

(1) This study aimed to investigate the association between child abuse and oral habits in adolescents in Mongolia. (2) A cross-sectional survey was conducted with children and their caregivers in Ulaanbaatar, Mongolia. Parents of 770 children enrolled in two public schools in Ulaanbaatar, Mongolia, completed questionnaires regarding the physical and psychological abuse that their children were subjected to and the presence of poor oral habits (biting nails/lips/pens and bruxism). Multivariable Poisson regression models were fitted with adjustment for age, gender, age of the mother, parental education, family income level, birth order, and living status with grandparents. (3) Biting nails/lips/pens and bruxism were reported by 39.0% and 17.5% of the respondents, respectively. Biting nails/lips/pens was significantly associated with physical abuse but was not significantly associated with psychological abuse (prevalence ratio, PR [95% confidence interval, CI]: 1.44 [1.07–1.95] and 1.34 [0.98–1.83], respectively). However, bruxism was not associated with physical or psychological abuse (PR [95% CI]: 1.16 [0.77–1.77] and 1.04 [0.68–1.61], respectively). (4) Child abuse was associated with biting habits among Mongolian adolescents.
Ergonomic Improvements to Agricultural Harvest Baskets to Reduce the Risk of Musculoskeletal Disorders among Farmers
Authors: Seo Mintae , Kim Hyocher , Jung Wongeon
Published: August 2022

Typical harvesting baskets (TB) are used in various agricultural workplaces; however, no study to date has reported their effect on the musculoskeletal system. Therefore, this study aimed to evaluate the effects of a novel basket with attached rotational handles (RHB) to help alleviate the work-related physical burden of farmers. We analyzed the surface electromyograms (EMGs) of seven muscles, evaluated the subjective discomfort levels and locally perceived discomfort (LPD) scores to investigate the discomfort in the whole body and seven hand muscles, respectively. The EMGs showed that muscle activity decreased in five muscles (flexor carpi ulnaris, extensor carpi radialis, lateral triceps, middle deltoid, and upper trapezius) and increased in two (biceps brachii and erector spinae) when the RHB was used (p < 0.05). The subjective discomfort score for the RHB decreased compared to that for TB (p < 0.001). The LPD scores also decreased, and the RHB and TB scores ranged from 1.25–1.40 and 3.1–3.25, respectively. The use of the RHB may prevent wrist bending, and reduce the activity of certain muscles while increasing the activity of other muscles. Therefore, it is necessary to conduct training and to evaluate the working posture while considering the affected muscles.
Association between Bone-Related Physiological Substances and Oral Function in Community-Dwelling Older People
Authors: Nakamura Misa , Imaoka Masakazu , Tazaki Fumie , Nakao Hidetoshi , Hida Mitsumasa , Kono Ryohei , Kanemoto Hideki , Takeda Masatoshi
Published: August 2022

Background: Oral dysfunction is related to long-term cares including activities of daily living. The objective of this study was to determine the association between oral function and the bone-related physiological substances osteocalcin (OC) and insulin-like growth factor-1 (IGF-1). Methods: The study participants were 139 community-dwelling older people in Japan. Evaluation of oral dysfunction was based on subjective judgment by each participant. Blood analysis included OC, IGF-1, and albumin. Results: Univariate and multiple logistic analyses showed that IGF-1 was significantly associated with a “decline in masticatory function” (p = 0.0074 and p = 0.0308, respectively). Receiver operating characteristic curve analysis of IGF-1 levels revealed a threshold score of 108 ng/mL (p < 0.01) for discriminating a “decline in masticatory function”. Logistic regression analysis revealed that participants with an IGF-1 level ≤108 ng/mL had an odds ratio of 4.31 (p < 0.05) for a “decline in masticatory function”. No significant association was found between the OC level and oral dysfunction. Conclusions: These results suggest a possible relationship between lower serum IGF-1 levels and a decline in masticatory dysfunction in community-dwelling older people.
Severity and Treatment Difficulty of Impacted Maxillary Canine among Orthodontic Patients in Riyadh, Saudi Arabia
Authors: Baidas Laila Fawzi , Alshihah Nada , Alabdulaly Rwan , Mutaieb Sara
Published: August 2022

Background: The current study aimed to evaluate the severity and treatment difficulty of impacted maxillary canines and their relationship with gender, age group, and bucco-palatal position. Methods: A retrospective cross-sectional study was conducted from 2017 to 2021. Patients’ data and panoramic radiography were obtained from the orthodontic clinic at King Saud University’s Dental University Hospital in Riyadh, Saudi Arabia. The severity factors and treatment difficulty index were used to assess the impacted maxillary canines. Statistical analyses were performed utilizing a chi-square test for categorical variables and an independent t-test for numerical variables, and a p-value of ≤0.05. Results: There were 171 impacted maxillary canines in total, with a female-to-male subject ratio (11:8) and a mean age (18.7 years). Overall, 77.2% of impacted upper canines were found to be palatal. The severity of canine impaction parameters showed no significant sex or age group predilection. Buccally impacted maxillary canines were characterized by a preferable angulation to the midline, compared to the palatally impacted maxillary canines (p = 0.012). The horizontal overlap of the impacted maxillary canine cusp tip revealed a significant association with the bucco-palatal position of the impacted canine (p < 0.001). Palatal impaction was located more frequently in sectors 3 and 4. Male patients were found to have a higher total score in terms of the treatment difficulty index relative to females (p = 0.046). Conclusion: Despite the severity parameters having revealed no significant gender predilection, males were found to have higher treatment difficulty in maxillary canine impaction than females. The severity of the palatally impacted canine is greater than that of buccal impaction in terms of angulation to the midline and horizontal overlap.