bluuebunny/crossref_metadata_2025 huggingface dataset:
Fixation stability comparison of bone screws based on thread design: buttress thread, triangle thread, and square thread
Authors: Feng Xiaoreng , Luo Zhaopei , Li Yupeng , Yao Yiyi , Qi Weichen , Chen Bin , Liang Hongfeng
Published: August 2022

Background

The influence of thread profile on the fixation stability of bone screws remains unclear. This study aimed to compare the fixation stability of screws with different thread profiles under several loading conditions.

Methods

Bone screws that differed in thread profile (buttress, triangle, and square thread) only were made of stainless steel. Their fixation stabilities were evaluated individually by the axial pullout test and lateral migration test, besides, they were also evaluated in pairs together with a dynamic compression plate and a locking plate in polyurethane foam blocks under cyclic craniocaudal and torsional loadings.

Results

The triangle-threaded and square-threaded screws had the highest pullout forces and lateral migration resistance. When being applied to a dynamic compression plate, higher forces and more cycles were required for both triangle- and square-threaded screws to reach the same displacement under cyclic craniocaudal loading. On the other hand, the triangle-threaded screws required a higher torque and more cycles to reach the same angular displacement under cyclic torsional loading. When being applied to a locking plate, the square-threaded screws needed higher load, torque, and more cycles to reach the same displacement under both cyclic craniocaudal and torsion loadings.

Conclusions

The triangle-threaded screws had superior pullout strength, while square-threaded screws demonstrated the highest lateral migration resistance. Moreover, dynamic compression plate fixation with triangle- and square-threaded screws achieved more favorable fixation stability under craniocaudal loading, while triangle-threaded screws demonstrated superior fixation stability under torsional loading. Locking plate fixation with a square-threaded screw achieved better fixation stability under both loading types.
S6K1 amplification confers innate resistance to CDK4/6 inhibitors through activating c-Myc pathway in patients with estrogen receptor-positive breast cancer
Authors: Mo Hongnan , Liu Xuefeng , Xue Yu , Chen Hongyan , Guo Shichao , Li Zhangfu , Wang Shuang , Li Caiming , Han Jiashu , Fu Ming , Song Yongmei , Li Dan , Ma Fei
Published: August 2022

Background

CDK4/6 inhibitors combined with endocrine therapy has become the preferred treatment approach for patients with estrogen receptor-positive metastatic breast cancer. However, the predictive biomarkers and mechanisms of innate resistance to CDK4/6 inhibitors remain largely unknown. We sought to elucidate the molecular hallmarks and therapeutically actionable features of patients with resistance to CDK4/6 inhibitors.

Methods

A total of 36 patients received palbociclib and endocrine therapy were included in this study as the discovery cohort. Next-generation sequencing of circulating tumour DNA in these patients was performed to evaluate somatic alterations associated with innate resistance to palbociclib. Then the candidate biomarker was validated in another independent cohort of 104 patients and publicly available datasets. The resistance was verified in parental MCF-7 and T47D cells, as well as their derivatives with small interfering RNA transfection and lentivirus infection. The relevant mechanism was examined by RNA sequencing, chromatin immunoprecipitation and luciferase assay. Patient-derived organoid and patient-derived xenografts studies were utilized to evaluated the antitumor activity of rational combinations.

Results

In the discovery cohort, S6K1 amplification (3/35, 9%) was identified as an important reason for innate resistance to CDK4/6 inhibitors. In the independent cohort, S6K1 was overexpressed in 15/104 (14%) patients. In those who had received palbociclib treatment, patients with high-expressed S6K1 had significantly worse progression free survival than those with low S6K1 expression (hazard ratio = 3.0, P = 0.0072). Meta-analysis of public data revealed that patients with S6K1 amplification accounted for 12% of breast cancers. Breast cancer patients with high S6K1 expression had significantly worse relapse-free survival (hazard ratio = 1.31, P < 0.0001). In breast cancer cells, S6K1 overexpression, caused by gene amplification, was sufficient to promote resistance to palbociclib. Mechanistically, S6K1 overexpression increased the expression levels of G1/S transition-related proteins and the phosphorylation of Rb, mainly through the activation of c-Myc pathway. Notably, this resistance could be abrogated by the addition of mTOR inhibitor, which blocked the upstream of S6K1, in vitro and in vivo.

Conclusions

S6K1 amplification is an important mechanism of innate resistance to palbociclib in breast cancers. Breast cancers with S6K1 amplification could be considered for combinations of CDK4/6 and S6K1 antagonists.
Anti-mullerian hormone levels before and after ovarian drilling in polycystic ovary syndrome: has this an effect on fertility?
Authors: Javedani Masroor Mojgan , Sheybani Hossein , Sheybani Shiva , Abolghasem Nastaran
Published: August 2022

Background

Polycystic ovary syndrome (PCOS) is the most common endocrine, metabolic, and multi-causal disorder in the reproductive period with a possible genetic origin. Women with PCOS are characterized by oligo-ovulation, clinical or biochemical hyperandrogenism, and polycystic ovaries. Women with PCOS have an increased number of antral follicles. Anti-Mullerian hormone (AMH), a dimeric glycoprotein produced from the granulosa cells of the pre-antral and antral follicles, is elevated in PCOS. AMH has been implicated in two stages of follicle dysfunction that lead to the development of PCOS. The level of AMH decreases following ovarian drilling in patients with PCOS. The present study compared the level of AMH before and after Laparoscopic ovarian drilling (LOD) in patients with PCOS and its effect on fertility.

Materials and methods

This cohort study was carried out on 84 women with PCOS who underwent LOD in Akbarabadi Hospital in Tehran in 2020. Demographic characteristics, AMH, and estradiol levels were determined before surgery and compared with the amount one week after surgery. The effect of AMH level on pregnancy rate was also evaluated.

Results

The mean age of the patients was 29.01 ± 4.01 years. The mean Body Mass Index (BMI) of the patients was 26.33 ± 4.14 kg/m2. The results showed that the mean AMH level decreased significantly after ovarian drilling (P-value < 0.001). Menstrual cycle distribution was significantly different before and after LOD (P < 0.001). None of the variables had an effect on the pregnancy (P-value > 0.05). Oligomenorrhea in the previous menstrual period might cause AMH levels to increase by 3.826 units after LOD (P-value < 0.001).

Conclusion

Measuring serum AMH concentration before treatment can be a useful tool to predict LOD outcomes. This can help in selecting the patient for treatment.

Trial registration

The project was found to be under the ethical principles and the national norms and standards for conducting research in Iran with the approval ID and issue date of IR.IUMS.FMD.REC.1397.206 and 2018.08.26 respectively, which has been registered with the research project number 2766 in the Vice-Chancellor for Research and Technology Development of Iran University of Medical Sciences, School of Medicine, Tehran, Iran. URL: https://ethics.research.ac.ir/EthicsProposalView.php?id=34791.
GNIP1 functions both as a scaffold protein and an E3 ubiquitin ligase to regulate autophagy in lung cancer
Authors: Zhou Feifei , Liu Yufeng , Ai Wenqian , Wang Yanan , Gan Mingxi , Jiang Qingkun , Han Tianyu , Wang Jian-Bin
Published: August 2022

Background

Glycogen-Interacting Protein 1 (GNIP1), an E3 ligase, is a member of the tripartite motif (TRIM) family proteins. Current studies on GNIP1 mainly focus on glycogen metabolism. However, the function and molecular mechanisms of GNIP1 in regulating autophagy still remains unclear. This study aimed to investigate the regulatory mechanism of GNIP1 in regulating autophagy in non-small cell lung cancer (NSCLC).

Methods

Crystal violet staining assays were used to evaluate the ability of cell growth and proliferation. Transwell and scratch wound healing assays were used to evaluate the cell migration ability. The protein expressions were measured by western blot and immunohistochemistry. Co-immunoprecipitation assays determined the protein–protein interactions. The in vivo effect of GNIP1 on tumor growth was determined by xenograft assay.

Results

We found that GNIP1 was overexpressed in tumor tissues and the expression level of GNIP1 was related to the poor prognosis and the survival time of NSCLC patients. In non-small cell lung cancer (NSCLC), GNIP1 increased proliferation and migration of cancer cells by promoting autophagy. Mechanistic studies indicated that GNIP1, as a scaffold protein, recruited BECN1 and LC3B to promote the formation of autophagosomes. Besides, GNIP1 mediated the degradation of 14-3-3ζ, the negative regulator of VPS34 complex, thus promoting autophagy. Overexpressing GNIP1 promoted tumorigenesis and enhanced autophagy in xenograft models.

Conclusion

GNIP1 promotes proliferation and migration of NSCLC cells through mediating autophagy, which provides theoretical basis for targeting GNIP1 as anti-cancer drugs.
Genomic analysis of single nucleotide polymorphisms in malaria parasite drug targets
Authors: Gill Jasmita , Sharma Amit
Published: August 2022

Malaria is a life-threatening parasitic disease caused by members of the genus Plasmodium. The development and spread of drug-resistant strains of Plasmodium parasites represent a major challenge to malaria control and elimination programmes. Evaluating genetic polymorphism in a drug target improves our understanding of drug resistance and facilitates drug design. Approximately 450 and 19 whole-genome assemblies of Plasmodium falciparum and Plasmodium vivax, respectively, are currently available, and numerous sequence variations have been found due to the presence of single nucleotide polymorphism (SNP). In the study reported here, we analysed global SNPs in the malaria parasite aminoacyl-tRNA synthetases (aaRSs). Our analysis revealed 3182 unique SNPs in the 20 cytoplasmic P. falciparum aaRSs. Structural mapping of SNPs onto the three-dimensional inhibitor-bound complexes of the three advanced drug targets within aaRSs revealed a remarkably low mutation frequency in the crucial aminoacylation domains, low overall occurrence of mutations across samples and high conservation in drug/substrate binding regions. In contrast to aaRSs, dihydropteroate synthase (DHPS), also a malaria drug target, showed high occurrences of drug resistance-causing mutations. Our results show that it is pivotal to screen potent malaria drug targets against global SNP profiles to assess genetic variances to ensure success in designing drugs against validated targets and tackle drug resistance early on.

Graphical Abstract
Clostridioides difficile toxin is infrequently detected in inflammatory bowel disease and does not associate with clinical outcomes
Authors: Bernard Rachel , Hammami Muhammad B. , Arnold Forest W. , Mcgrath Brian , Patel Alieysa , Wuerth Brandon , Nicholson Maribeth R. , Rao Krishna , Micic Dejan
Published: August 2022

Introduction

Clostridioides difficile infection (CDI) rates and outcomes can vary based on differences in testing strategy. Our aim was to assess the prevalence of toxin detection in inflammatory bowel disease (IBD) when compared to those without IBD. Secondly, the clinical outcomes of CDI in IBD were assessed using two-step testing strategies.

Methods

We included patients undergoing CDI testing from four academic centers in the United States between January 1, 2018 and June 30, 2020. First the prevalence of toxin detection was compared between individuals with IBD and those without IBD. Secondly, among patients with IBD a primary composite outcome of abdominal colectomy, admission to an intensive care unit (ICU) or death within 30 days of C. difficile testing was assessed across the three categorical groups (screen positive/toxin positive, screen positive/toxin negative and screen negative assay) resulting from the two-step testing strategy.

Results

When comparing individuals with a positive screening assay, patients with IBD were less likely to have toxin detected by enzyme immunoassay (EIA) as compared to the non-IBD population (22/145 (15.2%) vs. 413/1144 (36.1%), p < 0.0001). Among all patients with IBD (n = 300), twenty-five (8.3%) had a screen positive assay/toxin positive assay, 136 (45.3%) had a screen positive/toxin negative assay and 139 (46.3%) had a negative screening assay. No significant difference in the primary composite outcome was detected across the three groups (p = 0.566).

Conclusion

When compared to those without IBD, patients with IBD have a reduced proportion of cases of C. difficile with toxin positivity. Differences in clinical outcomes among patients with IBD were not detected and limited by the infrequent detection of expressed toxin.
Acute exacerbation of idiopathic hypereosinophilic syndrome following asymptomatic coronavirus disease 2019: a case report
Authors: Suzuki Satoshi , Suzuki Keiko , Ichikawa Takaya , Takahashi Kae , Minami-Hori Masako , Tanino Yoko
Published: August 2022

Background

Previous research has suggested that some autoimmune diseases develop after the occurrence of coronavirus disease 2019. Hypereosinophilic syndrome is a rare disease presenting with idiopathic eosinophilia and multiple organ involvement, including the skin, lungs, gastrointestinal tract, heart, and nervous system. The diagnosis of idiopathic hypereosinophilic syndrome poses a dilemma because clinical manifestation and serum biomarkers are similar to those of eosinophilic granulomatosis with polyangiitis. Only a few cases have been reported where coronavirus disease 2019 may have caused the new onset or exacerbation of eosinophilic granulomatosis with polyangiitis or idiopathic hypereosinophilic syndrome.

Case presentation

We present the case of a 48-year-old Japanese woman with history of asthma who developed deteriorating symptoms of insidiously developed idiopathic hypereosinophilic syndrome following asymptomatic coronavirus disease 2019. She developed acute-onset back pain, tachycardia, abdominal discomfort, loss of appetite, weight loss, skin rash on the back, and numbness of the extremities 3 days after the quarantine period. Extreme hypereosinophilia with multiple abnormal findings including pulmonary ground-glass opacity lesions and mononeuritis multiplex was consistent with hypereosinophilic syndrome. Normal cellularity with eosinophilic proliferation in the bone marrow and negative FIP1L1–PDGFRA raised the diagnosis of idiopathic hypereosinophilic syndrome. Although the patient tested negative for anti-neutrophilic cytoplasmic antibodies and skin biopsy was negative for vasculitis, eosinophilic granulomatosis with polyangiitis could not be excluded. Since glucocorticoids are a standard therapy for both idiopathic hypereosinophilic syndrome and eosinophilic granulomatosis with polyangiitis, we initiated glucocorticoids following a multidisciplinary discussion.

Conclusion

Although the relationship between asymptomatic coronavirus disease 2019 and acute idiopathic hypereosinophilic syndrome exacerbation was uncertain, the chronological order of the symptomatic development suggested a possible link. More clinical cases and population-based studies are needed to determine the potential effect of coronavirus disease 2019 on autoimmune diseases.
Introduction of ROSA robotic-arm system for total knee arthroplasty is associated with a minimal learning curve for operative time
Authors: Bolam Scott M. , Tay Mei Lin , Zaidi Faseeh , Sidaginamale Raghavendra P. , Hanlon Michael , Munro Jacob T. , Monk A. Paul
Published: August 2022

Purpose

The introduction of robotics for total knee arthroplasty (TKA) into the operating theatre is often associated with a learning curve and is potentially associated with additional complications. The purpose of this study was to determine the learning curve of robotic-assisted (RA) TKA within a multi-surgeon team.

Methods

This prospective cohort study included 83 consecutive conventional jig-based TKAs compared with 53 RA TKAs using the Robotic Surgical Assistant (ROSA) system (Zimmer Biomet, Warsaw, Indiana, USA) for knee osteoarthritis performed by three high-volume (> 100 TKA per year) orthopaedic surgeons. Baseline characteristics including age, BMI, sex and pre-operative Kellgren-Lawrence graded and Hip-Knee-Ankle Axis were well-matched between the conventional and RA TKA groups. Cumulative summation (CUSUM) analysis was used to assess learning curves for operative times for each surgeon. Peri-operative and delayed complications (infection, periprosthetic fracture, thromboembolism, and compromised wound healing) and revisions were reviewed.

Results

The CUSUM analysis for operative time demonstrated an inflexion point after 5, 6 and 15 cases for each of the three surgeons, or 8.7 cases on average. There were no significant differences (p = 0.53) in operative times between the RA TKA learning (before inflexion point) and proficiency (after inflexion point) phases. Similarly, the operative times of the RA TKA group did not differ significantly (p = 0.92) from the conventional TKA group. There was no discernible learning curve for the accuracy of component planning using the RA TKA system. The average length of post-operative follow-up was 21.3 ± 9.0 months. There was one revision for instability in the conventional TKA group and none in the RA TKA group. There were no significant difference (p > 0.99) in post-operative complication rates between the conventional TKA and RA TKA groups.

Conclusions

The introduction of the RA TKA system was associated with a learning curve for operative time of 8.7 cases. Operative times between the RA TKA and conventional TKA group were similar. The short learning curve implies this RA TKA system can be adopted relatively quickly into a surgical team with minimal risks to patients.
Multiparametric liver MRI for predicting early recurrence of hepatocellular carcinoma after microwave ablation
Authors: Zhang Zhaohe , Yu Jie , Liu Sisi , Dong Linan , Liu Tiefang , Wang Haiyi , Han Zhiyu , Zhang Xiaojing , Liang Ping
Published: August 2022

Background

High early recurrence (ER) of hepatocellular carcinoma (HCC) after microwave ablation (MWA) represents a sign of aggressive behavior and severely worsens prognosis. The aim of this study was to estimate the outcome of HCC following MWA and develop a response algorithmic strategy based on multiparametric MRI and clinical variables.

Methods

In this retrospective study, we reviewed the records of 339 patients (mean age, 62 ± 12 years; 106 men) treated with percutaneous MWA for HCC between January 2014 and December 2017 that were evaluated by multiparametric MRI. These patients were randomly split into a development and an internal validation group (3:1). Logistic regression analysis was used to screen imaging features. Multivariate Cox regression analysis was then performed to determine predictors of ER (within 2 years) of MWA. The response algorithmic strategy to predict ER was developed and validated using these data sets. ER rates were also evaluated by Kaplan–Meier analysis.

Results

Based on logistic regression analyses, we established an image response algorithm integrating ill-defined margins, lack of capsule enhancement, pre-ablative ADC, ΔADC, and EADC to calculate recurrence scores and define the risk of ER. In a multivariate Cox regression model, the independent risk factors of ER (p < 0.05) were minimal ablative margin (MAM) (HR 0.57; 95% CI 0.35 – 0.95; p < 0.001), the recurrence score (HR: 9.25; 95% CI 4.25 – 16.56; p = 0.021), and tumor size (HR 6.21; 95% CI 1.25 – 10.82; p = 0.014). Combining MAM and tumor size, the recurrence score calculated by the response algorithmic strategy provided predictive accuracy of 93.5%, with sensitivity of 92.3% and specificity of 83.1%. Kaplan–Meier estimates of the rates of ER in the low-risk and high-risk groups were 6.8% (95% CI 4.0 – 9.6) and 30.5% (95% CI 23.6 – 37.4), respectively.

Conclusion

A response algorithmic strategy based on multiparametric MRI and clinical variables was useful for predicting the ER of HCC after MWA.
The impact of biochar on wood-inhabiting bacterial community and its function in a boreal pine forest
Authors: Qu Zhao-lei , Li Xiao-li , Ge Yan , Palviainen Marjo , Zhou Xuan , Heinonsalo Jussi , Berninger Frank , Pumpanen Jukka , Köster Kajar , Sun Hui
Published: August 2022

Biochar is considered to be a possible means of carbon sequestration to alleviate climate change. However, the dynamics of the microbial community during wood decomposition after biochar application remain poorly understood. In this study, the wood-inhabiting bacterial community composition and its potential functions during a two-year decomposition period after the addition of different amounts of biochar (0.5 kg m−2 and 1.0 kg m−2), and at different biochar pyrolysis temperatures (500 °C and 650 °C), in a boreal Scots pine forest, were analyzed using Illumina NovaSeq sequencing combined with Functional Annotation of Prokaryotic Taxa (FAPROTAX). The results showed that the wood decomposition rates increased after biochar addition to the soil surface in the second year. Treatment with biochar produced at high temperatures increased the diversity of wood-inhabiting bacteria more than that produced at low temperatures (P < 0.05). The wood-inhabiting bacterial diversity and species richness decreased with decomposition time. The biochar treatments changed the wood-inhabiting bacterial community structure during the decomposition period. The pyrolysis temperature and the amount of applied biochar had no effect on the bacterial community structure but shifted the abundance of certain bacterial taxa. Similarly, biochar application shifted the wood-inhabiting bacterial community function in the first year, but not in the second year. The wood-inhabiting bacterial community and function were affected by soil pH, soil water content, and soil total nitrogen. The results provide useful information on biochar application for future forest management practices. Long-term monitoring is needed to better understand the effects of biochar application on nutrient cycling in boreal forests.
Predictors of mental health deterioration from pre- to post-COVID-19 outbreak
Authors: Rius Ottenheim Nathaly , Pan Kuan-Yu , Kok Almar A. L. , Jörg Frederike , Eikelenboom Merijn , Horsfall Melany , Luteijn Rob A. , van Oppen Patricia , Rhebergen Didi , Schoevers Robert A. , Penninx Brenda W. J. H. , Giltay Erik J.
Published: August 2022

Background

Mental health was only modestly affected in adults during the early months of the COVID-19 pandemic on the group level, but interpersonal variation was large.

Aims

We aim to investigate potential predictors of the differences in changes in mental health.

Method

Data were aggregated from three Dutch ongoing prospective cohorts with similar methodology for data collection. We included participants with pre-pandemic data gathered during 2006–2016, and who completed online questionnaires at least once during lockdown in The Netherlands between 1 April and 15 May 2020. Sociodemographic, clinical (number of mental health disorders and personality factors) and COVID-19-related variables were analysed as predictors of relative changes in four mental health outcomes (depressive symptoms, anxiety and worry symptoms, and loneliness), using multivariate linear regression analyses.

Results

We included 1517 participants with (n = 1181) and without (n = 336) mental health disorders. Mean age was 56.1 years (s.d. 13.2), and 64.3% were women. Higher neuroticism predicted increases in all four mental health outcomes, especially for worry (β = 0.172, P = 0.003). Living alone and female gender predicted increases in depressive symptoms and loneliness (β = 0.05–0.08), whereas quarantine and strict adherence with COVID-19 restrictions predicted increases in anxiety and worry symptoms (β = 0.07–0.11).Teleworking predicted a decrease in anxiety symptoms (β = −0.07) and higher age predicted a decrease in anxiety (β = −0.08) and worry symptoms (β = −0.10).

Conclusions

Our study showed neuroticism as a robust predictor of adverse changes in mental health, and identified additional sociodemographic and COVID-19-related predictors that explain longitudinal variability in mental health during the COVID-19 pandemic.
Multicentre, England-wide randomised controlled trial of the ‘Foundations’ smartphone application in improving mental health and well-being in a healthcare worker population
Authors: Gnanapragasam Sam N. , Tinch-Taylor Rose , Scott Hannah R. , Hegarty Siobhan , Souliou Emilia , Bhundia Rupa , Lamb Danielle , Weston Danny , Greenberg Neil , Madan Ira , Stevelink Sharon , Raine Rosalind , Carter Ben , Wessely Simon
Published: August 2022

Background

Healthcare workers (HCWs) have faced considerable pressures during the COVID-19 pandemic. For some, this has resulted in mental health distress and disorder. Although interventions have sought to support HCWs, few have been evaluated.

Aims

We aimed to determine the effectiveness of the ‘Foundations’ application (app) on general (non-psychotic) psychiatric morbidity.

Method

We conducted a multicentre randomised controlled trial of HCWs at 16 NHS trusts (trial registration number: EudraCT: 2021-001279-18). Participants were randomly assigned to the app or wait-list control group. Measures were assessed at baseline, after 4 and 8 weeks. The primary outcome was general psychiatric morbidity (using the General Health Questionnaire). Secondary outcomes included: well-being; presenteeism; anxiety; depression and insomnia. The primary analysis used mixed-effects multivariable regression, presented as adjusted mean differences (aMD).

Results

Between 22 March and 3 June 2021, 1002 participants were randomised (500:502), and 894 (89.2%) followed-up. The sample was predominately women (754/894, 84.3%), with a mean age of 44⋅3 years (interquartile range (IQR) 34–53). Participants randomised to the app had a reduction in psychiatric morbidity symptoms (aMD = −1.39, 95% CI −2.05 to −0.74), improvement in well-being (aMD = 0⋅54, 95% CI 0⋅20 to 0⋅89) and reduction in insomnia (adjusted odds ratio (aOR) = 0⋅36, 95% CI 0⋅21 to 0⋅60). No other significant findings were found, or adverse events reported.

Conclusions

The app had an effect in reducing psychiatric morbidity symptoms in a sample of HCWs. Given it is scalable with no adverse effects, the app may be used as part of an organisation's tiered staff support package. Further evidence is needed on long-term effectiveness and cost-effectiveness.
Biomechanical energetics of terrestrial locomotion in California sea lions (Zalophus californianus)
Authors: Kerr Sarah J. , Fish Frank E. , Nicastro Anthony J. , Zeligs Jenifer A. , Skrovan Stephanie , Leftwich Megan C.
Published: August 2022

Pinnipedia, an order of semi-aquatic marine mammals, adapted a body design that allows for efficient aquatic locomotion but limited terrestrial locomotion. Otariids, like the California sea lion (Zalophus californianus), have enlarged forelimbs and can bring their hindlimbs under the body to locomote quadrupedally on land, but phocids (true seals) have reduced forelimbs and are unable to bring their hindlimbs beneath them during terrestrial locomotion. Because of these differences, phocids are expected to have greater energetic costs when moving on land compared with otariids. The mechanical costs of transport (COT) and power outputs of terrestrial locomotion were first obtained from one male and two female adult California sea lions through video recording locomotion sequences across a level runway. The center of mass, along with six other anatomical points, were digitized to obtain variables such as velocity (V), amplitude of heave (A) and the frequency (f) of oscillations during the locomotion cycle. These variables represent the principal parameters of a biomechanical model that computes the power output of individuals. The three California sea lions in this study averaged a power output of 112.04 W and a COT of 0.63 J kg−1 m−1. This footage was compared against video footage previously recorded of three phocid species (harbor seal, gray seal and northern elephant seal). Power output and mechanical COT were compared for all four pinniped species by tracking the animals' center of mass. The quadrupedal gait of sea lions showed lower vertical displacements of the center of mass, and higher velocities compared with the terrestrial gait of phocids. Northern elephant seals, gray seals and harbor seals showed significantly higher COT and power outputs than the sea lions. California sea lions locomote with lower energetic costs, and thus higher efficiency compared with phocids, proving that they are a mechanically intermediate species on land between terrestrial mammals and phocids. This study provides novel information on the mechanical energy exerted by pinnipeds, particularly California sea lions, to then be used in future research to better understand the limitations of these aquatic mammals.
Locomotor resilience through load-dependent modulation of muscle co-contraction
Authors: Günzel Yannick , Schmitz Josef , Dürr Volker
Published: August 2022

Terrestrial locomotor behavior in variable environments requires resilience to sudden changes in substrate properties. For example, walking animals can adjust to substantial changes in slope and corresponding changes in load distribution among legs. In insects, slope-dependent adjustments have mainly been examined under steady-state conditions, whereas the transition dynamics have been largely neglected. In a previous study, we showed that steady-state adjustments of stick insects to ±45 deg slopes involve substantial changes in joint torques and muscle activity with only minor changes in leg kinematics. Here, we took a close look at the time course of these adjustments as stick insects compensate for various kinds of disturbances to load distribution. In particular, we tested whether the transition from one steady state to another involves distinct transition steps or follows a graded process. To resolve this, we combined simultaneous recordings of whole-body kinematics and hindleg muscle activity to elucidate how freely walking Carausius morosus negotiated a step-change in substrate slope. Step-by-step adjustments revealed that muscle activity changed in a graded manner as a function of body pitch relative to gravity. We further show analogous transient adjustment of muscle activity in response to destabilizing lift-off events of neighboring legs and the disappearance of antagonist co-activation during crawling episodes. Given these three examples of load-dependent regulation of antagonist muscle co-contraction, we conclude that stick insects respond to both transient and sustained changes in load distribution by regulating joint stiffness rather than through distinct transition steps.
Rational Illiquidity and Consumption: Theory and Evidence from Income Tax Withholding and Refunds
Authors: Gelman Michael , Kariv Shachar , Shapiro Matthew D. , Silverman Dan
Published: August 2022

Low liquidity and a high marginal propensity to consume are tightly linked. This paper analyzes this link in the context of income tax withholding and refunds. A theory of rational cash management with income uncertainty endogenizes the relationship between illiquidity and the marginal propensity to consume, and can explain the finding that households tend to spend tax refunds as if they valued liquidity, yet do not act to increase liquidity by reducing their withholding. The theory is supported by individual-level evidence based on financial account records, including a positive correlation between the size of tax refunds and the marginal propensity to consume out of those refunds. (JEL E21, G51, H24, H31)
Measuring Racial Discrimination in Bail Decisions
Authors: Arnold David , Dobbie Will , Hull Peter
Published: August 2022

We develop new quasi-experimental tools to measure disparate impact, regardless of its source, in the context of bail decisions. We show that omitted variables bias in pretrial release rate comparisons can be purged by using the quasi-random assignment of judges to estimate average pretrial misconduct risk by race. We find that two-thirds of the release rate disparity between White and Black defendants in New York City is due to the disparate impact of release decisions. We then develop a hierarchical marginal treatment effect model to study the drivers of disparate impact, finding evidence of both racial bias and statistical discrimination. (JEL J15, K42)
Methods Matter: p-Hacking and Publication Bias in Causal Analysis in Economics: Reply
Authors: Brodeur Abel , Cook Nikolai , Heyes Anthony
Published: August 2022

In Brodeur, Cook, and Heyes (2020) we present evidence that instrumental variable (and to a lesser extent difference-in-difference) articles are more p-hacked than randomized controlled trial and regression discontinuity design articles. We also find no evidence that (i) articles published in the top five journals are different; (ii) the “revise and resubmit” process mitigates the problem; (iii) things are improving through time. Kranz and Pütz (2022) apply a novel adjustment to address rounding errors. They successfully replicate our results with the exception of our shakiest finding: after adjusting for rounding errors, bunching of test statistics for difference-in-difference articles is now smaller around the 5 percent level (and coincidentally larger at the 10 percent level). (JEL A14, C12, C52)
Virtual non-contrast images from dual-energy CT angiography of the abdominal aorta and femoral arteries: comparison with true non-contrast CT images
Authors: Lee Min Hee , Park Hee Jin , Kim Ji Na , Kim Myung Sub , Hong Seok Woo , Park Jai Hyung , Kang Chang Ho
Published: August 2022

Objectives:

To assess whether virtual non-contrast (VNC) computed tomography (CT) images acquired from dual-energy CT (DECT) have sufficient image quality to replace true non-contrast (TNC) CT images in CT angiography (CTAG).

Methods:

This study enrolled 63 consecutive patients who underwent a CTAG that included a single-energy non-contrast scan, followed by a post-contrast DECT scan. Comparison of attenuation and noise between TNC and VNC images was made by drawing circular regions of interest (ROI) on a picture archiving and communication system (PACS) workstation within the aortic lumen at the levels of the renal arteries, the aortic bifurcation and right femoral artery. Mean attenuation and image noise (one standard deviation) were registered in Hounsfield units (HU). The VNC images were subjectively evaluated for artifacts such as subtraction of calcifications or architectural distortion based on TNC image as a standard of reference.

Results:

Most attenuations of the VNCs were higher than TNC, except right femoral artery of reader 2. Most image noises of TNC were higher than VNC, except abdominal aorta in reader 1. In qualitative image analysis, mean scores of VNC according to the 5-point scale were 3.68 and 3.63 (reader 1 and reader 2, respectively) which mean good to excellent to diagnose.

Conclusion:

HUs and VNC image noises are different from TNC images in CTAG. VNC images have sufficient image quality to replace TNC images in the diagnosis of calcific lesions.

Advances in knowledge:

VNC images acquired from DECT have image quality adequate to replace TNC images in the diagnosis of the calcific lesion on the CTAG. VNC images based on DECT can avoid excessive and unnecessary patient exposure to radiation during CTAG.
Lei geral das agências: A nova reforma regulatória no Brasil
Authors: Ramalho Pedro Ivo Sebba , Lopes André Vaz , Taveira Rodrigo Abrão Veloso
Published: August 2022

O Brasil promoveu recentemente uma ampla reforma organizacional e institucional das agências reguladoras federais. Essa reforma regulatória, representada pela chamada Lei Geral das Agências (Lei nº 13.848/2019), buscou aprimorar a governança regulatória por meio da definição de novas estruturas e procedimentos obrigatórios para as agências independentes, objetivando a promoção da qualidade regulatória. Este artigo teve como objetivo avaliar os reflexos da nova legislação no funcionamento das agências reguladoras federais, particularmente às ferramentas de transparência e participação, e da harmonização e do aprimoramento do processo decisório, utilizados nas boas práticas regulatórias. Mediante a análise documental e de entrevistas estruturadas com atores-chave nas 11 agências federais, foi feita uma comparação entre o período anterior à lei e o cenário regulatório após um ano de sua vigência. Os dados coletados indicam a consolidação da consulta pública, da análise de impacto regulatório e da transparência na tomada de decisões, aspectos tratados pela nova legislação. Tais práticas já faziam parte da cultura das agências independentes, mas a edição da nova legislação refletiu em uma sedimentação dessas ações essencial à adoção de regulamentos com qualidade, em linha com as ferramentas utilizadas para a better regulation, adotadas pelos entes regulatórios europeus. Esse novo cenário desvelado pela pesquisa aponta um potencial avanço no modelo de governança regulatória brasileiro no nível federal, o que pode gerar efeitos positivos na credibilidade e confiança das agências reguladoras independentes.
Laparoscopic Umbilical Hernia repair in male patients with abdominal obesity
Authors: AlMulhim Abdulrahman Saleh , Memon Abdul Qadeer
Published: August 2022

Objective: Obesity is a global health problem, and obese patients are subject to developing abdominal wall hernias. There are few prospective studies comparing the laparoscopic method of umbilical hernia mesh repair between abdominal obesity patients and normal abdominal waist patients. The aim of this study was to evaluate the short-term outcomes (operative time, early complications and hospital stay) in the patients having laparoscopic hernia repair with abdominal obesity.

Methods: This prospective cohort study was conducted at King Fahad Hospital Hofuf, Kingdom of Saudi Arabia from June 2014 to June 2021. Fifty four (54) adult male patients with umbilical hernia were included in this study. The patients were divided into two groups: Group-A: Patients with abdominal obesity (n=26), and Group-B: Patients without abdominal obesity (n=28). All the patients underwent laparoscopic repair of umbilical hernia. The patients with abdominal obesity were defined as those having an abdominal girth more than 102 centimeters.

Results: No significant differences were observed as related to age, co-morbidity and risk factors between the two groups. The statistically significant difference between the two groups observed was related to the mean operative time and the mean hospital stay.

Conclusion: Laparoscopic umbilical hernia repair can be safely performed in abdominal obesity in male patients without an additional risk of complications.

doi: https://doi.org/10.12669/pjms.38.7.6470

How to cite this:Al-Mulhim AS, Memon AQ. Laparoscopic Umbilical Hernia repair in male patients with abdominal obesity. Pak J Med Sci. 2022;38(7):1776-1779. doi: https://doi.org/10.12669/pjms.38.7.6470

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The Greek Law on Strategic Investments in Light of the FDI Screening Regulation: Will the Greek Investment Regime’s Openness Endure?
Authors: Fotopoulos Dionysis N.
Published: August 2022

Attracting Foreign Direct Investment (FDI) is vital for Greece’s growth in the aftermath of a lengthy economic crisis and the Covid-19 pandemic. Concurrently, the European Union (EU) seeks to safeguard essential bloc-wise security interests that are compromised by third country investors, especially Chinese ones, sprawling across member states – a policy concern substantiated in normative text as recently as 2019 by the enactment of Regulation 2019/452. The latter set the basic pan-European contour in terms of member states’ cooperation for the screening of FDI. A year later, the Greek Parliament voted for Law 4864/2021 on Strategic Investments, with a view to incentivizing aspiring high-profile investors. In that regard, the main objective of the present policy paper is to examine how Law 4864/2021 interplays with Regulation 2019/452. The paper will conclude that the Greek liberalized framework will have to align in the future with the European trend of extended screening over FDI, notwithstanding Law 4864/2021 moving into the opposite direction.
Putin’s Great Patriotic War: Russia’s Securitization of the West and Humiliation Narratives Surrounding the 2022 Invasion of Ukraine
Authors: Fusiek Dawid Aristotelis
Published: August 2022

The paper seeks to analyze Vladimir Putin’s securitization of the West in his speech at a military parade in Moscow’s Red Square on the 9th of May 2022. Thereby, the article employs political discourse analysis and Copenhagen School’s securitization theory to show how Putin uses speech acts to transform the West into an existential threat and justify the adoption of extraordinary measures, such as the 2022 Russian invasion of Ukraine. Furthermore, the research refers to Richard Sakwa’s concepts of “cold peace” and “neo-revisionism” to make sense of the emergence of such discourse. By using humiliation narratives and evoking patriotic images, Putin exacerbates a sense of victimhood and historical right in the Russian audience that emphasizes the state’s isolationism and historic role to fight against the “Nazified” West and Ukraine. Putin also promotes an image of Russia’s superiority, supporting cultural and civilizational pluralism and diversity ending the pre-existing Western universalism. The paper concludes that with these practices, the Russian president manages to not only successfully securitize the West but also spur significantly the public’s support for his war.
Political Responsiveness in Crisis Period, Sustainability and ESG in EU: The Initiatives of Next Generation EU and the Potentials for Programming Period 2021-2027
Authors: Taliouris Evangelos , Manasakis Constantine
Published: August 2022

Crisis in Europe used to generate significant socioeconomic and political changes in institutions and states cooperation, especially in post war period. During the last decade, the financial crisis, in combination with climate change and the COVID-19 pandemic has challenged significantly EU, while at the same time, the Lisbon Treaty in 2009 provided solutions as well as the political will of “being together is better”. The EU Green Deal indicated that cooperation is a prerequisite for EU as a whole and at the same time, it is a fact in combination with the Sustainable Development Goals (SDGs). Moreover, Covid-19 has indicated the need for an effective policy response in order to deal with this health crisis. The main issue for EU is how to inspire and provide a sustainable, fair and social inclusive future for the member states and next generation. The EU via the “Transforming our world: the 2030 Agenda for Sustainable Development” booklet analyzes its international position in sustainable development issues, while the Next Generation EU is an important policy and financial tool in order to improve the common policies in new programming period 2021-2027.
Dual Instruments and Sequential Products of Observables
Authors: Gudder Stan
Published: August 2022

We first show that every operation possesses an unique dual operation and measures an unique effect. If a and b are effects and J is an operation that measures a, we define the sequential product of a then b relative to J. Properties of the sequential product are derived and are illustrated in terms of Lüders and Holevo operations. We next extend this work to the theory of instruments and observables. We also define the concept of an instrument (observable) conditioned by another instrument (observable). Identity, state-constant and repeatable instruments are considered. Sequential products of finite observables relative to Lüders and Holevo instruments are studied.
Review of the literature on the dangers of zolpidem use, its potential for abuse and addiction
Authors: Skowrońska Katarzyna , Chmura Anna , Pondel Kamil , Cyboran Katarzyna , Baciur Patrycja
Published: August 2022

Introduction and purpose of the work : Commonly Z-drugs, especially zolpidem , are considered safer than short-acting drugs benzodiazepines . Recently, more and more is said about the abuse of these relatively harmless drugs. The aim of this study is to collect scientific reports on the increasing abuse of these drugs.

State of knowledge: There are many reports of zolpidem abusers and addicts in the available literature . These people belong to three groups of patients: the first is people with insomnia who develop tolerance, the second is recreational use, and the third is people with mental disorders. Taking increased doses of zolpidem causes a psychostimulant effect, increased activity during sleep, up to delusions and psychotic symptoms. Rapid discontinuation of high doses of the drug should be performed in a hospital setting. Summary : Physicians should be advised to remain vigilant during therapy with group Z drugs and to check that the patient is not receiving the drug from multiple sources and is not taking zolpidem in supra-therapeutic doses .
Economic and Environmental Implications of Biomass Commercialization in Agricultural Processing
Authors: Li Bin , Boyabatlı Onur , Avcı Buket
Published: August 2022

Motivated by the agricultural industries, this paper studies the economic and environmental implications of biomass commercialization; that is, converting organic waste into a saleable product from the perspective of a processor that uses a commodity input to produce both a commodity output and biomass. We characterize the economic value of biomass commercialization and examine how input and output spot price uncertainties affect this value. Using a model calibration, we find that lower input spot price variability or higher output spot price variability or correlation between the two spot prices increases this value for a typical palm oil mill. To measure the environmental impact, we use total expected carbon emissions resulting from profit-maximizing decisions and characterize the change in total expected emissions after commercialization. Our analysis reveals that, although higher biomass demand or biomass price always increases the value of biomass commercialization, these changes are not necessarily environmentally beneficial as they may increase the emissions associated with biomass commercialization. We also characterize conditions under which biomass commercialization is environmentally beneficial or harmful; that is, it leads to a reduction or an increase in the total expected emissions, respectively. In comparison with the existing understanding which does not take into account optimization of operational decisions, our analysis highlights two types of misconceptions (and characterizes the specific conditions under which they appear): (i) we would mistakenly think that biomass commercialization is environmentally beneficial when it is not, and (ii) we would mistakenly think that biomass commercialization is environmentally harmful when it is not.

This paper was accepted by Victor Martínez-de-Albéniz, operations management.

Supplemental Material: The online appendix is available at https://doi.org/10.1287/mnsc.2022.4518 .