bluuebunny/crossref_metadata_2025 huggingface dataset:
A multifaceted analysis of spent mushroom substrate of selected oyster mushrooms for enzymatic activity, proximate composition, and antimicrobial activity
Authors: Afsar Mashaim , Zia Afia , Us Salam Muhammad Baseer , Ahmad Muhammad Nauman , Khan Ayaz Ali , Ul Haq Taqweem , Aziz Tariq , Alasmari Abdullah F
Published: February 2024

The global market for mushrooms is growing due to its nutritional enrichment, potential usage as a bioremediation, enzyme production, and functional food development. However, the leftover post-harvest mushroom substrate (SMS) generates certain environmental concerns. This study aimed to investigate the potential of SMS obtained from two oyster mushroom species—Pleurotus ostreatus and Pleurotus djamor. These were examined regarding sustainability by analyzing their lignocellulosic enzyme production, cellulose yield, antimicrobial properties, and proximate composition. The findings for both P. ostreatus and P. djamor showed higher activity of amylase, that is, 0.3 U (μmol/min) and 0.7 U (μmol/min), respectively, compared to activity of cellulase, which showed 0.3 U (μmol/min) and 0.5 U (μmol/min), respectively. SMS showed the highest activity of lignocellulosic enzymes, compared to non-SMCs and controls at p ≤ 0.00 and ≤0.01), proving fungual mycelia as the precursor of enzymes activity, as no mushroom is cultivated due to least enzymatic activity. The results for proximate analysis of SMCs showed a significant difference from non-SMCs. The findings for P. djmor revealed protein (1.23%), fats (1.3%), and ash (8.11), which were significantly higher than in P. ostreatus. A positive co-relation of 52% was established between SMCs with amylase, while a correlation of 20% was observed with cellulase, depicting an impact of mycelia in the breakdown of protein for amylase production. The SMC samples were also subjected to antibacterial analysis against Staphylococcus aureus, E. coli, and Xanthomonas. A higher minimum inhibition concentration (MIC) was recorded for P. djamor, that is, 8.80 mm, 11.66 mm, and 9.04 mm, compared to P. ostreatus, which showed its highest MIC as 9.18 mm, 9.30 mm, and 9.28 mm for S. aureus, E. coli, and Xanthomonas, respectively. It was evident from the study that SMC has a potential of being utilized for bioremediation, as it is therapeutically active against pathogens. Additionally, Pleurotus spp. is of great interest because of its ability to produce high nutritive value, cellulose yield, and a vast amount of lignocellulosic enzymes. The current experiment recommends the use of distilled water for mushroom farming, as enzymatic activities can significantly be affected by pH and buffers. Furthermore, the spent compost, being rich nutritionally, can be used for soil enrichment or as a biofertilizer.
Some new fuzzy query processing methods based on similarity measurement and fuzzy data clustering
Authors: Nguyen Tan Thuan  , Tran Thi Thuy Trinh  , Doan Van Ban  , Truong Ngoc Chau  , Nguyen Thi Anh Phuong  , Nguyen Truong-Thang
Published: February 2024

In relational and object-oriented database systems there is always data that is naturally fuzzy or uncertain. However, to deal with complex data types with fuzzy nature, these systems have many limitations. Therefore, in order to represent and manage fuzzy data, it is necessary to have a fuzzy interrogation system to facilitate non-expert users. To solve this challenge, the paper proposes two different approaches to increase the flexibility of the fuzzy interrogation system. Firstly, based on similarity measures and fuzzy logic, we develop three fuzzy query processing algorithms for single-condition and multi-condition cases such as FQSIMSC (Fuzzy Query Sim Single Condition), FQSIMMC (Fuzzy Query Sim Multi-Condition) and FQSEM (Fuzzy Query SEM). Secondly, we combine the fuzzy clustering algorithm EMC (Expectation maximization Coefficient) and the query processing algorithm that is based on fuzzy partitions FQINTERVAL (Fuzzy Query Interval). With this approach, we not only improve query processing cost but also support applications and devices equipped with intelligent interactive function that easily interacts with the fuzzy query system. The results of our theoretical and experimental analysis, it can be seen that both the proposed methods significantly reduce the processing time and memory space for a data set (extracted from UCI) that has a fuzzy and incomplete natural element with the resulting data size being optimal
A new method of failure analysis
Authors: Alexandrov Sergei , Rynkovskaya Marina , Bajmuratov Ismet , Kalistratov Ruslan , Pylkin Ivan
Published: February 2024

The present paper develops a new failure analysis method under plane strain conditions considering a generalized linear yield criterion. The yield criterion and the stress equilibrium equations constitute a hyperbolic system of equations. It is shown that two auxiliary variables satisfy the equation of telegraphy. Simple analytical relationships connect these variables and the radii of curvature of the characteristic curves. The calculated radii of curvature allow for the corresponding characteristic net to be constructed. Then, the stress field is determined using another set of analytical relationships. Thus, a numerical procedure is only necessary for solving the equation of telegraphy. This equation can be integrated by the method of Riemann. In particular, Green’s function is the Bessel function of zero order. A simple example illustrates the general method.
A composição financeira do déficit público
Authors: DALL’ACQUA FERNANDO MAIDA , BRESSER-PEREIRA LUIZ
Published: February 2024

RESUMO A partir dessa hipótese, este artigo critica a teoria convencional sobre déficit público e apresenta um modelo alternativo de análise macroeconômica dos efeitos do déficit público enfatizando sua composição interna entre juros e gastos reais.
How can low germination rates in Amazon chicory seeds be overcome?
Authors: Rosário Isabelle Caroline Bailosa do , Arruda Renata da Silva , Silva Andressa de Oliveira , Gomes Rafaelle Fazzi
Published: February 2024

ABSTRACT: Eryngiumfoetidum exhibits low germination rates and heterogeneous seed maturation. Thus, in order to reduce problems during seedling production, a number of treatments can be used. The aim of this study was to assess the physiological quality of Amazon chicory seeds submitted to water immersion treatments. Two experiments were conducted, under controlled temperature (25 ºC) and nursery conditions, with six treatments (0, 12, 24, 36 and 48 hours) in each. A completely randomized design was used in the controlled condition and random blocks in the nursery. The following were assessed: germination/emergence speed index (GSI/ESI), mean germination/emergence time (MGT/MET), germination/emergence percentage, normal seedling development, fresh weight and dry weight. The data were submitted to linear and second-order polynomial regression analysis in the R statistical program. Under controlled conditions, Maximum GSI was 4.65 after 18.03 hours of immersion, and for MGT, 12 h of immersion provided a shorter average time (13.76 days) and obtained 39.26% of germinated seeds after 26.27 h of immersion, as well as 19.34% of normal seedlings in 27.97 h. In the nursery, a lower MET was observed after 48 h and mean fresh and dry weight were higher after 12 h (18.47 and 1.44 g, respectively). Thus, water immersion for 12 hours resulted in a shorter germination time and higher fresh and dry weight, and the treatment may have helped reactivate embryo metabolism and growth.
Nutritional composition and antioxidant and cancer chemopreventive activities of fruits of Psidiummyrtoides (O. Berg)
Authors: Cassiano Camile Zanichelli da Costa , Soares Karla Lírio , Batista Patrícia Berilli , Marques Stephano , Maróstica Júnior Mário Roberto , Bogusz Junior Stanislau , Núñez Oscar , Fronza Marcio , Endringer Denise Coutinho , Scherer Rodrigo
Published: February 2024

ABSTRACT: Psidiummyrtoides fruits food industries produce by-products such as liqueurs, ice cream, jellies, and juices. However, there needs to be more information about the nutritional composition of fruits, as well as the biological potential, mainly of seed flour.Therefore, the present study aimed to evaluate the nutritional composition and antioxidant and cancer chemopreventive activities of pulp and seed of fruits of Psidiummyrtoides. Moisture content, total minerals and mineral profile, total lipids and fatty acids profile, total proteins, dietary fiber (soluble and insoluble) and total carbohydrates were analyzed. Vitamins C and complex B, organic acids and the profile of phenolic compounds were also determined. DPPH and ABTS methods evaluated the antioxidant activity, and cancer chemopreventive activity was evaluated by quinone reductase induction and NF-κB inhibitory activity. The fruits are a good source of thiamine, iron, phosphorus, potassium, fiber, and protein. Linoleic acid was the major fatty acid in both pulp and seed. Quercetin (1600 µg/100g) and pyrogallol(819 µg/100g) were the major phenolic compounds pulp and seed, respectively. The fruits showed strong antioxidant capacity, mainly the seeds, and the ability to induce quinone reductase activity, highlighting a cancer chemopreventive activity. Seed flour has a high potential to enrich food, in addition to valuing agro-industrial by-products, where we can highlight 25%, 13% and 37% of the recommended daily intake for iron (1.6 mg/100g), phosphorus (58 mg/100g) and vitamin B1 (450 µg/100g), respectively, in addition to antioxidant and cancer chemopreventive activities.
Revisiting and comparing the Carnot Cycle and the Otto Cycle
Authors: Kroetz Tiago
Published: February 2024

The reversibility of the Carnot Cycle makes it the most efficient thermodynamic cycle to convert energy as heat into work operating between two thermal reservoirs at different temperatures. The Otto cycle represents an idealization of the processes in the spark-ignition 4-stroke internal combustion engine operation. Some aspects of these two crucial thermodynamic cycles will be presented here in a comparative manner. This highlights why the Carnot Cycle does not offer advantages over the Otto Cycle in the operation of real thermal engines when we consider the efficiency and work done per cycle dependent on the compression ratio of the cycles.
Madres buscadoras: de la sospecha a la legitimidad
Authors: Gesteira Soledad
Published: February 2024

Resumen: Este artículo describe y analiza el surgimiento del activismo de un grupo de mujeres que denuncian el robo de sus hijos/as al nacer en Argentina y los/as están buscando. A partir de observaciones y entrevistas con estas madres buscadoras se analizan, por un lado, las nociones a las que apelan para dotar de legitimidad a sus reclamos y oponerse a la sospecha que se erige sobre ellas como malas madres que entregaron y/o abandonaron a sus hijos/as. Y, por otro lado, las condiciones de posibilidad para la emergencia de este activismo y las particularidades que le imprime la configuración local de los derechos humanos y de los derechos de las mujeres. Se postula como hipótesis que las prácticas de robo de niños/as que denuncian estas mujeres constituyen formas específicas de violencia inscriptas en tramas sociales de desigualdad de género, clase y edad.
Morphological and biochemical changes in squash fruits with fruiting induced by cytokinin and auxin
Authors: Santos Gisele L. dos , Pereira Francisco H. F. , Araújo Railene H. C. R. , Oliveira Agda M. F. de , Lins Hamurábi A. , Silva Antonio G. C. da , Barros Júnior Aurélio P. , Ribeiro João E. da S.
Published: February 2024

ABSTRACT The use growth regulators from the cytokinins and auxins group has been shown to increase the productivity of certain crops, as they may be involved in the expression of plant sex, although little is known about the action of auxin IBA and cytokinin CPPU on the fruiting of squash, as well as the interaction between both, which makes this field of study promising. Thus, the objective of this study was to induce the fruiting in Atlas squash, by means of different forms of application and proportions of cytokinin and auxin and to evaluate their effects on the physical-chemical characteristics of the fruits. The treatments were composed of six proportions associated with growth regulators and of two forms of application. The experimental design was a randomized block design with four replicates. At 72 days after transplanting, growth and fruit quality analyses were performed. The behavior of the hormones is modulated by their concentration and place of application, and the effect may be beneficial or not. The proportions 50/50% via leaf and 75/25% via ovary were the ones that increased the growth and parameters of fruit quality. The application of CPPU via leaf at higher concentration in proportion decreases quality characteristics such as vitamin C, carotenoids and starch.
Literacy practices and representations in the comic books genre produced by undergraduate students in Rural Education
Authors: Santos Milena dos , Silva Cícero da
Published: February 2024

ABSTRACT In this paper, we analyze literacy practices and representations in the comic books genre produced by undergraduates taking the Rural Education degree. The research is based on literacy theories and is situated in the applied field of language. This is a participatory research, with a qualitative-interpretative approach. The corpus consists of texts from six examples of the comic books genre, and (transcribed) excepts from three interviews conducted with research collaborators. Because this genre has a union of different language systems (verbal and non-verbal), the research revealed traces of literacy that students have in their life experiences. As the research collaborators come from different communities located in rural areas, they present diverse and peculiar literacy practices and representations.
O EU EM NÓS: MARCAS E APROXIMAÇÕES DE UMA DIRETORA NO PROCESSO PSICOTERÁPICO
Authors: Vidal Gabriela Pereira , Ferracini Lucio Guilherme
Published: February 2024

RESUMO O objetivo deste trabalho foi aprofundar as marcas e aproximações de uma diretora no processo psicoterápico. Trata-se de um estudo de caso psicodramático utilizando o registro documental de sessões de psicoterapia bipessoal de três pacientes atendidas anteriormente pela primeira autora e os relatos da própria autora sobre suas percepções nos atendimentos. Foram apresentadas reflexões que demonstram resultados positivos sobre as aproximações na relação psicoterapêutica mediante o humor, a poesia e a música como formas de conexão entre psicodramatista e cliente.
Experiencias de carrera dual y salud mental en estudiantes deportistas de alto rendimiento
Authors: Devoto Maximiliano , Jordana Anna , Bauzá Joan Pons , Zamora-Solé Rocío , Regüela Susana , Kegelaers Jolan , De Brandt Koen , Torregrossa Miquel
Published: February 2024

RESUMEN Estudiantes deportistas de alto rendimiento enfrentan demandas en distintas áreas de su vida al realizar una Carrera Dual (CD). En este estudio exploramos la relación entre experiencias de CD y Salud Mental (SM). Un total de 128 estudiantes deportistas de alto rendimiento respondieron los cuestionarios DCES, MHLS-12, y MHC-SF. Los resultados muestran que las experiencias de CD y alfabetización en SM influyen positivamente en el estado de SM de los estudiantes deportistas. Estos resultados subrayan la importancia de potenciar la interacción investigación-aplicación, involucrando al entorno para fortalecer la SM a través de experiencias positivas y alfabetización.
Long-Acting Drug Delivery Technologies for Meloxicam as a Pain Medicine
Authors: Annaji Manjusha , Mita Nur , Heard Jessica , Kang Xuejia , Poudel Ishwor , Boddu Sai H. S. , Tiwari Amit K. , Babu R. Jayachandra
Published: February 2024

Meloxicam, a selective COX-2 inhibitor, has demonstrated clinical effectiveness in managing inflammation and acute pain. Although available in oral and parenteral formulations such as capsule, tablet, suspension, and solution, frequent administration is necessary to maintain therapeutic efficacy, which can increase adverse effects and patient non-compliance. To address these issues, several sustained drug delivery strategies such as oral, transdermal, transmucosal, injectable, and implantable drug delivery systems have been developed for meloxicam. These sustained drug delivery strategies have the potential to improve the therapeutic efficacy and safety profile of meloxicam, thereby reducing the frequency of dosing and associated gastrointestinal side effects. The choice of drug delivery system will depend on the desired release profile, the target site of inflammation, and the mode of administration. Overall, meloxicam sustained delivery systems offer better patient compliance, and reduce the side effects, thereby improving the clinical applications of this drug. Herein, we discuss in detail different strategies for sustained delivery of meloxicam.
Papillon-Lefèvre Syndrome: 17-Year Dental Follow-Up. Case Report
Authors:   Division of Oral and Maxillofacial Radiology, School of Dentistry, Universidad Científica del Sur, Lima, Perú., Melchor-Soto Miguel , Arriola-Guillén Luis Ernesto ,   Division of Orthodontics and Division of Oral and Maxillofacial Radiology, School of Dentistry, Universidad Científica del Sur, Lima, Perú., Ruíz-Mora Gustavo Armando ,   Division of Orthodontics and Division of Oral and Maxillofacial Radiology, School of Dentistry, Universidad Científica del Sur, Lima, Perú,   Division of Orthodontics, Faculty of Dentistry, Universidad Nacional de Colombia, Bogotá D.C, Colombia., Rodríguez-Cárdenas Yalil Augusto ,   Division of Oral and Maxillofacial Radiology, School of Dentistry, Universidad Científica del Sur, Lima, Perú,   Division of Oral and Maxillofacial Radiology, School of Dentistry, Universidad Nacional de Colombia, Bogotá D.C, Colombia., Melchor-Soto Jesús ,   Division of Odonto-pediatric Dentistry, School of Dentistry, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México., Romero-Quintana José ,   Faculty of Biological and Chemical Sciences, Postgraduate Program in Biotechnology and Postgraduate Program in Biomedical Sciences, Autonomous University of Sinaloa, Culiacán, Sinaloa, Mexico., Aliaga-Del Castillo Aron ,   Department of Orthodontics, Bauru Dental School. University of São Paulo, Brazil.
Published: February 2024

Introduction: The present report describes the case of a 12-year-old patient with 17-year follow-up who was previously diagnosed with Papillon-Lefèvre Syndrome (PLS), which is a rare autosomal recessive irregularity in the cathepsin C gene (CTSC) characterized by palmoplantar hyperkeratosis and premature loss of primary and permanent teeth. Case Report: A specific mutation in the c.203 T > G gene inducing loss of function leading to PLS was detected, as was a mutation in the HLA-DRB1*11 allele, which is associated with this syndrome. There is no consanguinity of the parents, and the siblings are entirely healthy. Early identification of the main characteristics of this syndrome is imperative. Accurate diagnosis by genetic analysis allows differential diagnoses and timely comprehensive dental treatment. Conclusions: Additionally, it allows consultation with a dermatologist to maintain or improve the quality of life of patients with this condition due to progressive worsening and severity of the main physical manifestations. Keywords: Papillon-Lefevre Disease; Keratoderma, Palmo-plantar; Cathepsin C; Periodontitis; Skin Diseases, Genetic; Case reports
Reconstruyendo el campo de los estudios en evaluación de Políticas Públicas en las universidades públicas españolas
Authors: Alarcón-González Francisco Javier , Flores-Candorcio Juan José
Published: February 2024

En enero de 2023 entró en vigor en España la Ley de Institucionalización de la Evaluación de PolíticasPúblicas en la Administración General del Estado. Esta ley da un paso importante al establecer la evaluaciónde políticas públicas (EPP) como elemento para mejorar el uso eficaz y eficiente de los recursospúblicos y dotar de una mayor transparencia al proceso de toma de decisiones. Para ello es necesariocontar con personal capacitado para realizar las evaluaciones. El objetivo de este artículo es presentarcómo se estructura la formación universitaria dentro de las universidades públicas españolas en EPP ycómo los diferentes grados universitarios vinculados mayoritariamente a la Ciencia Política introducenla formación en análisis y evaluación de políticas públicas. A partir de la observación y análisis de lapresencia de estas formaciones en las universidades, este estudio muestra cómo la EPP se encuentra enuna fase bastante incipiente en el desarrollo dentro de los estudios de ciencia política, siendo un enfoquerelativamente joven en España dentro de la Ciencia Política y parcialmente limitado a unas cuantasuniversidades. El volumen de créditos formativos en análisis y evaluación de políticas públicas sobre eltotal de la formación es marginal. Esto significa que, en términos de competencias alcanzadas al final dela formación universitaria y de la transferencia de esos aprendizajes al puesto de trabajo, depende de larealización de la formación universitaria en determinadas universidades.
Evaluación de la satisfacción sexual en la población LGBTIQ Ecuador
Authors: Champutiz-Quintana Kathy Ayde , Checa-Olmos Juan Carlos , Lucas-Matheu Manuel , Bastidas-Champutiz Santiago Bryan
Published: February 2024

La satisfacción sexual se expresa como una evaluación, para manifestar el nivel de agrado a la vidasexual. Los estudios en la sexualidad de la población LGBTIQ han sido poco profundizados, gruposrodeados de tabúes, víctimas de una represión y condenas sociales; afectando las diferentes dimensionesde la salud, como la satisfacción sexual. Este trabajo investiga la escala unidimensional de la satisfacciónsexual en la población LGBTIQ Ecuador. Se analiza cómo esta escala es afectada al correlacionarsecon las diferentes variables sociodemográficas. Se utiliza una metodología cuantitativa, correlacionaldescriptiva y prospectiva. Se aplica: cuestionario a 625 personas residentes en Ecuador con recogida dedatos sociodemográficos, Índice de Satisfacción Sexual (ISS).Los resultados de la escala unidimensional de satisfacción sexual muestran que el 33 % de la poblacióntiene satisfacción sexual, el 60,5 % experimenta insatisfacción sexual y un 6,6 % reporta una experienciasevera de estrés en esta área. Se observa una menor insatisfacción sexual en los residentes de la ciudad deQuito, en hombres, en personas homosexuales, en aquellos con un nivel educativo más alto y en individuosafrodescendientes, con un valor de p < 0,005, lo que indica una significancia estadística.Estos resultados reflejan un nivel negativo de satisfacción sexual en la población estudiada. Por lo tanto,se hace necesario continuar investigando la satisfacción sexual para comprender las causas y los factoresque inciden en la insatisfacción sexual. Es crucial visibilizar las necesidades y los apoyos que requiereesta comunidad en un entorno seguro y respetuoso, especialmente considerando los constantes actos dediscriminación que enfrentan.
Biogeografia em abordagens ecológicas e geográficas: análise dos artigos do I Simpósio Brasileiro de Biogeografia (2020)
Authors: Oliveira Marianne Silva , Carvalho Luiz Felipe Rodrigues
Published: February 2024

A Biogeografia como campo científico interdisciplinar enfrenta desafios para delinear suas bases conceituais, teóricas e metodológicas sob perspectiva da ciência Geográfica. A utilização de dados relacionados a diferentes campos científicos resulta em abordagens distintas que são sintetizadas na literatura como abordagens ecológicas e geográficas, sendo o objetivo deste estudo identificar o tipo de abordagem desenvolvida nos artigos apresentados no I Simpósio Brasileiro de Biogeografia (2020). Os dados relativos aos aspectos bióticos, abióticos e antrópicos utilizados nos artigos foram identificados em suas diferentes combinações que resultam em abordagens distintas e para as quais foram identificados os métodos de análise e escalas espaciais. Verificou-se a predominância da abordagem geográfica contemplada em 80% dos artigos nas quais estão presentes os componentes abióticos e antrópicos associados aos bióticos. A combinação de métodos qualitativos e quantitativos ocorreu em 64% do total de artigos e houve predominância de estudos para escala local em 48%. O desafio para integração de dados relacionados às sociedades humanas e suas intervenções no ambiente é ponto comum evidenciado em estudos sob perspectiva da Geografia. Neste contexto, a integração de dados antrópicos com utilização de métodos quali-quantitativos por meio da abordagem geográfica que foi predominante nos artigos do I Simpósio Brasileiro de Biogeografia constitui relevante contribuição aos debates em escala nacional que visam promover a ampliação das bases conceituais, teóricas e metodológicas deste campo científico interdisciplinar.
Identificação da tendência das emissões de gases do efeito estufa no estado do Amazonas: uma abordagem com suavização exponencial
Authors: Lima Filho Francisco Soares
Published: February 2024

Este artigo foca na importância da compreensão das emissões de dióxido de carbono equivalente (CO2e) no estado do Amazonas, destacando as potenciais implicações para políticas públicas. Utilizando análise de séries temporais de dados climáticos, o estudo investiga padrões de variabilidade e busca prever mudanças de curto e longo prazo nas emissões de CO2e. O objetivo é identificar os modelos de suavização exponencial mais adequados para prever a tendência das emissões de CO2e resultantes de mudanças no uso da terra na Amazônia durante o período de 1990 a 2021. Além disso, o artigo avalia a precisão dos modelos selecionados. Os resultados enfatizam a necessidade crítica de desenvolver modelos eficazes de previsão e estimativa em diversas áreas, destacando sua relevância na informação dos processos de formulação de políticas.
Morphological Diversity and Production of Six Sago (Metroxylon spp.) Accessions from Tana Luwu, South Sulawesi, Indonesia
Authors: Masluki Masluki , Bintoro Mochamad Hasjim , Agusta Herdhata , Sudarsono Sudarsono
Published: February 2024

Tana Luwu area owns a relatively high diversity and production potential of sago, but it has not yet been managed optimally. This study aims to identify and analyze the diversity of morphological characteristics and the production of various sago accessions in Tana Luwu. Based on morphological characteristics, the sago palm in Tana Luwu is divided into two groups by the presence of thorns on the rachis and petioles. The presence of thorns has the largest coordinate axis angles, representing a higher diversity distance than other morphological characteristics. Some accessions show very high production potential, with a total production of 476.66 kg per dry tree starch. The average sago production in the six accessions is 276.40 kg per dry tree starch. Accessions with a yield potential > 200 kg per dry starch are observed in Kapa, Uso, Kasimpo, and Sabbe. Therefore, sago in these accessions is potentially developed to obtain superior varieties. Two significant morphological characteristics that contribute to sago production are stem height (r = 0.73) and the number of leaves (r = 0.78).
Barcoded and targeted cDNA library preparation for Oxford Nanopore Technologies sequencing v1
Authors: A Bamford Rosemary
Published: February 2024

The NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (NEB) was adapted for the purpose of adding Oxford Nanopore Technologies (ONT) compatible barcodes during reverse transcription of RNA. This is a useful process for multiplexing low input samples for ONT transcriptome library preparation. An optional step before ONT library preparation is the targeted enrichment of cDNA molecules using IDT hybridisation probes. Here we provide a protocol for this process based on the 'PacBio cDNA capture using IDT xGen Lockdown Probes' protocol. Using this approach, up to 100 samples can be barcoded with individual ONT barcodes via reverse transcription. Samples can then be pooled together and a cDNA PCR amplification performed. This allows sufficient material for cDNA enrichment and/or ONT ligation sequencing library preparation.
BAF_Protocol_006 On-Bead Peptide Cleanup (Digested by Other Method) v1
Authors: not provided nesf
Published: February 2024

This protocol is for using beads as a cleanup step after protein is digested using a different method (not directly on Sera-Mag beads), for example solution digest. The peptides are precipitated onto the beads to allow for removal of salts and detergents that will interfere with downstream mass spectrometry.
Absolute Illiteracy in European Union Policies. Exploring the Intersections of Gender, Migration and Education
Authors: Barroso Margarida M.
Published: February 2024

In the context of a study focused on the absolute illiteracy of migrant women in Europe, this article assesses the extent to which European Union policy instruments on gender, migration and education incorporate an intersectional perspective. The analysis was based in the content analysis of a selected group of EU policy documents and included i) the close and iterative reading and thematic codification of the texts; ii) the word frequency counting of relevant terms; and iii) the assessment of intersectionality through a set of quality criteria. Apart from revealing the invisibility of absolute illiteracy as a policy problem, results confirm previous evidence showing that the presence of intersectional approaches in EU public policy is still marginal. Some attempts to address the intersectionality of gender, migration or education inequalities have been identified in the selected documents, but a comprehensive policy framework to understand and address those inequalities is still lacking. The article adds up to the existing scholarship claiming for more inclusive policies in contemporary European societies.
Postmodernist collage as a method for regeneration of public spaces
Authors: Kuznetsov Ivan , Akhmedova Elena
Published: February 2024

Today's times are characterized by an unprecedented emancipation of creative searches, liberation from canons and stereotypes, a fundamental rejection of the traditions of creativity according to one model, a transition to creativity by «intuition» based on the conditions of a specific order, location, capabilities and desires. The modern architect is preoccupied with the creative search for his «element A» (according to K. Malevich), his creative method, which could indicate the path to creating a new form, a new artistic image. The individualistic principle, together with the desire to achieve unique originality in each new work, and at the same time the desire to accommodate cultural and artistic archetypes of various other eras in one volume-spatial architectural object or in a fragment of the urban architectural environment, adding new reinvented meanings to them and through this achieving architectural and artistic integrity directs us to search for new methods of creating diversity and uniqueness. The new method of «Postmodern Collage» now provides such creative possibilities.
The Infinity of Fedir Tetianych
Authors:   Taras Shevchenko National University of Kyiv, Kyiv, Ukraine, Yarmolenko Vladyslava
Published: February 2024

Ukrainian art in the late 20th century witnessed significant changes marked by the independence and originality of artists, responding to the repressive Soviet system. Fedir Tetianych, also known as Frypulia, personified a unique Ukrainian phenomenon from the 1970s to the early 21st century. Despite being an outsider during his lifetime, Tetianych's posthumous recognition has grown, with his works now featured in prominent contemporary art centers worldwide, including the prestigious Center Pompidou in Paris. The book «Fedir Tetianych. Frypulia», published in 2021 by the PinchukArtCentre research group, offers a comprehensive overview of Tetianych's life and artistic journey. Analytical essays by various authors explore Tetianych's artistic vision and personality, his concept of Frypulia, and the significance of his textual heritage. The archival collection of Tetianych's works, housed at the Modern Art Research Institute of the Ukrainian Academy of Arts, is also presented. This review focuses on the book's essays, delving into Tetianych's artistic development, unique concepts, and the innovative approach of Valerii Sukharuk in exhibiting Tetianych's work. As the first in-depth study of Tetianych, the book is essential for grasping his distinctive artistic vision and addressing the challenge of classifying his work within traditional artistic styles. It underscores the importance of fostering a broader discourse around Tetianych's groundbreaking contribution to art.
InSAR, Gravity, and Geomagnetic Observations Suggest the 2020 Mw 6.5 Stanley Earthquake Occurred on a Blind Strike-Slip and a Normal Fault
Authors: Zhao Jing-Jing , Chen Qiang , Yang Ying-Hui , Xu Qian , Li Pengfei , Xu Lang
Published: February 2024

The 2020 Mw 6.5 Stanley earthquake occurred in western North America and is the largest earthquake in Idaho state in the past ∼40 yr. The hypocenter is located at the western margin of the Centennial Tectonic Belt (CTB), which is a subprovince of the Basin and Range and characterized by a series of normal faults. In this study, the coseismic Interferometric Synthetic Aperture Radar observations, geophysical data, and aftershocks are jointly collected to estimate the seismogenic fault of the 2020 Stanley earthquake. The best-fitting fault models suggest the earthquake occurred on two blind faults. Slip on the main, west-dipping, seismogenic fault was sinistral and on the second fault predominantly normal slip with a minor component of right-lateral strike slip. Geophysical observations including gravity and geomagnetic data are consistent with the fault interpretations. The Coulomb failure stress (CFS) change indicates that the rupture of the main seismogenic fault had a positive triggering effect on the slip of the second fault and may have increased the rupture risk of the Sawtooth fault. Moreover, a potential seismic hazard risk zone with a notably low occurrence of aftershocks is found in the west of the central segment of the main seismogenic fault.
Los estudiantes de Agronomía elaboran miniclases y vídeos mediante aprendizaje colaborativo
Authors: López Puente Secundino , Mateos Álvarez Iván , López Ferreras Lorena , Ranilla García María José , Saro Higuera Cristina , Valdés Solís Carmen
Published: February 2024

Para motivar a los estudiantes de Agronomía (primer curso de Veterinaria) en el aprendizaje de algunos temas prácticos de la asignatura, fomentar el autoaprendizaje, el trabajo colaborativo y el espíritu crítico, desde el curso 2019-2020 se les propone que trabajen en grupos (3-4 estudiantes) para elaborar una miniclase y un vídeo educativo sobre una serie de temas del programa de prácticas adjudicados al azar a cada grupo, que exponen y defienden ante el resto de sus compañeros y de dos profesores. Cada estudiante, además, evalúa los trabajos presentados por el resto de los grupos. Desde el curso 2021-2022 se realiza una encuesta de satisfacción para cada actividad. El 100% de los estudiantes completaron ambas actividades y, en una escala de 0 a 5, se mostraron satisfechos con las miniclases (3.97±0.100 de media) y con los vídeos (3.94±0.104 de media). Además, las miniclases de sus compañeros les resultaron más divertidas que las clases impartidas por profesores (3.56±0.133) y consideran que los vídeos les permiten comprender mejor algunos conceptos (3.64± 0.109). Aunque exponer las miniclases y presentar los vídeos ante sus compañeros les resultó estimulante (3.17±0.127 y 3.21±0.107, respectivamente), los estudiantes no participaron en la discusión de los trabajos de sus compañeros. Todos los estudiantes participaron en el proceso de evaluación cruzada que implicaba evaluar a los compañeros y ser evaluados por ellos. Este proceso les pareció una buena experiencia, tanto para las miniclases (3.83±0.116) como para los vídeos educativos (3.85±0.121). Los coeficientes de correlación entre las notas concedidas por los profesores y por los estudiantes fueron significativos (0.5-0.6), aunque no se establecieron criterios de valoración. En conclusión, estas actividades parecen mejorar la motivación de los alumnos para el aprendizaje, pero la participación en la discusión de las actividades debe estimularse y la evaluación cruzada podría facilitarse mediante el empleo de rúbricas.
Metodología para construir una red de investigadores con base en teoría de redes complejas
Authors: Mesa-Mazo Mónica Jhoana , García-Usuga Jorge Mario , Rincón-Penagos Julián Andres
Published: February 2024

En este trabajo se implementó una herramienta computacional con base en teoría de redes y vigilancia científica-tecnológica que sirvan para visualizar las tendencias investigativas de una institución de educación superior: caso de estudio, Corporación universitaria empresarial Alexander von Humboldt. Para esto, se desarrolló un instrumento para la recolección de la información de los proyectos de investigación de la institución, con base en ésta se construyeron diferentes tipos de redes (palabras clave y autores). Finalmente, se hizo un análisis a cada una de éstas y se estudió la centralidad de las redes, obteniendo como resultado una red poco conectada con baja densidad. De igual forma, se identificaron las diferentes tendencias en investigación en la institución, así como los investigadores con más diversidad de temáticas
Intrauterine Transmission of Hepatitis B Cannot Be Ruled Out by A Single Negative Hepatitis B e Antigen (HBeAg) Result among Hepatitis B Surface Antigen (HBsAg) - Positive Pregnant Women
Authors: Chalid Maisuri Tadjuddin , Judistiani Tina Dewi , Syahril Rizalinda , Masadah Rina , Febriani Dwi Bahagia , Wahyuni Ridha , Turyadi Turyadi , Massi Muh Nasrum
Published: February 2024

BACKGROUND: The risk factors for intrauterine transmission of hepatitis B virus (HBV) in hepatitis B surface antigen (HBsAg)-positive pregnant women are poorly understood. Numerous factors are considered to be involved, including placental barrier, obstetric environment, high viral load, and positivity of hepatitis B e antigen (HBeAg). This study was conducted to investigate the role of placenta barrier, clinical, and viral factors in intrauterine transmission of HBV.METHODS: A cross-sectional study was conducted involving 1,353 pregnant women who underwent HBsAg screening. Eighty-four (6.2%) women were detected as HBsAg positive and were examined for HBsAg level, anti-HBs, anti-HBc, HBeAg/hepatitis B e antibody (anti-HBe) status, and HBV DNA presence in cord blood. Quantitative HBV DNA was analyzed using real-time polymerase chain reaction (PCR).RESULTS: Eighty-four of 1,353 subjects were HBsAg-positive. HBV DNA was positive in 28/84 (33.7%) maternal sera, 19/79 (24.05%) placental specimens, and 9/83 (10.84%) in cord blood. There were significant associations between HBV DNA in maternal serum (p=0.000) and placental tissue (p=0.000) with HBV DNA in the cord blood. No clinical factors were associated with HBV DNA transmission in cord blood. Sixty percent of viral load >5.3 log10 copies/mL were found in the cord blood, of which 43.8% HBeAg positive and 3.1% HBeAg negative.CONCLUSION: Reduced transmission via compartments established the placenta’s barrier function in mother-to-child transmission. A high maternal viral load and positive HBeAg were risk factors for intrauterine transmission, while negative HBeAg still has the possibility of transmission.KEYWORDS: mother-to-child transmission, hepatitis B virus, intrauterine
Effects of SGLT2-inhibitor on The Expression of MicroRNA-21, Transforming Growth Factor-β1, and Matrix Metalloproteinase-2 in The Process of Cardiac Fibrosis in Hyperglycemic Model Rats
Authors: Ridwan Muhammad , Dimiati Herlina , Syukri Maimun , Lesmana Ronny , Zaini Lia Meuthia
Published: February 2024

BACKGROUND: Sodium glucose co-transporter-2 inhibitor (SGLT2-i), a new oral antidiabetic drug, has been recommended for its morbidity and mortality benefits in patients with heart failure. This study aimed to determine the effect of acute SGLT2-i therapy on the relative ratio expression of microRNA-21 (miR-21), transforming growth factor-β1 (TGF-β1), and matrix metalloproteinase-2 (MMP-2) in the process of cardiac fibrosis in the hyperglycemia Wistar rat models compared to biguanide (metformin) therapy.METHODS: We used Streptozotocin (STZ) to induce hyperglycemia in Wistar rats (n=31), randomly divided into four groups: negative control (NC, n=4), positive control (PC, n=10), hyperglycemia plus metformin (M, n=8), and hyperglycemia plus empagliflozin (E, n=9). After seven weeks, the rats were sacrificed and the heart tissue was taken for microRNA and messenger RNA (mRNA) extraction, followed by reverse transcription quantitative real time polymerase chain reaction (RT-qPCR) examination. The data was analyzed using One-way ANOVA.RESULTS: Results showed a decreasing trend in the gene expression relative ratio of miR-21 (1.0 vs. 1.9; p=0.079) and TGF-β1 (0.9 vs. 3.2; p=0.145), but a significant increase in MMP-2 gene expression (1.3 vs. 0.7; p=0.002) in the SGLT2-i (empagliflozin) vs. biguanide (metformin) groups.CONCLUSION: Empagliflozin administration may play a significant role in preventing the occurrence of cardiac fibrosis in hyperglycemia.KEYWORDS: sodium glucose co-transporter-2 inhibitor, microRNA-21, transforming growth factor-β1, matrix metalloproteinase-2, cardiac fibrosis
Humanistyczny obraz starości. Przesłanki dla gerontologii humanistycznej. Refleksje nad starością. Tom 3
Authors: Dubas Elżbieta
Published: February 2024

W opracowaniu, będącym próbą naszkicowania obrazu starości w odniesieniu do wartości humanistycznych i jej holistycznego ujęcia, uwzględniono wybrane konteksty. Jednym z nich jest perspektywa trójjedni, a więc jedności ciała, psychiki i ducha, kolejnymi – połączona przestrzeń świata wewnętrznego i świata zewnętrznego oraz obiektywnego i subiektywnego wymiaru starości. W takim oglądzie nie sposób pominąć spojrzenia na starość w kontekście wielowymiarowego rozwoju oraz w perspektywie szeroko rozumianego uczenia się. Podjęto także próbę dostrzeżenia w starości piękna, choćby dlatego, że to dziś nie częsty sposób oglądu starości. Poświęcono także miejsce trudnym doświadczeniom egzystencjalnym, jakie dla człowieka starszego bywają codziennością, w szczególności chorobie Alzheimera. Kategoria „towarzyszenie w drodze” okazała się być niezwykle celna dla opisu relacji człowieka z człowiekiem na drodze ich życia i starości. Z dramatu doświadczania trosk egzystencjalnych wyprowadzona została perspektywa traktowania starości nie tylko jako zadania i wyzwania, ale także jako daru. Na starzenie się można spojrzeć także jako na dojrzewanie, stawanie się w pełni człowiekiem. I wreszcie ostatni kontekst, silnie przemawiający aktualnie, w XXI wieku – perspektywa ery cyfrowej, i tu również próba odnalezienia humanistycznej wizji starości. Wskazane konteksty uwypuklają godność osoby starszej oraz holistyczny wymiar starości, wynikający z jednostkowego doświadczania pełnego cyklu życia, zazębiania się doświadczeń na przestrzeni całego życia i splotu jednostkowych doświadczeń z bogatym spektrum wewnętrznych i zewnętrznych uwarunkowań starości i procesów starzenia się.
Program Bimbingan dan Konseling Hipotetik untuk Meningkatkan Kepercayaan Diri Siswa Sekolah Menengah Pertama
Authors: Adiamila Lingga Diany  , Setiawati  , Rina Nurhudi Ramdhani
Published: February 2024

Siswa SMP kelas 8 berada pada masa remaja awal yaitu berada pada usia 12-15 tahun yang dimana siswa perlu memenuhi tugas perkembangan masa remaja. Kepercayaan diri menjadi salah satu yang harus dipenuhi remaja dalam memenuhi tugas perkembangannya serta dapat menerima dirinya sendiri dan bagaimana cara bersikap dan bersosialisasi dengan lingkungan sekitar. Penelitian ini memiliki tujuan Mendeskripsikan kepercayaan diri siswa kelas delapan di SMP Negeri 9 Bandung, Mendeskripsikan kepercayaan diri siswa perempuan dan laki-laki kelas delapan di SMP Negeri 9 Bandung, Serta rancangan program bimbingan dan konseling yang dapat diimplementasikan dalam mengembangkan kepercayaan diri siswa kelas delapan di SMP Negeri 9 Bandung. Penelitian ini merupakan penelitian kuantitatif dengan metode deksriptif dengan variabel kepercayaan diri. sampel dalam penelitian ini berjumlah 329 siswa dengan teknik dalam pengambilan sampel menggunakan pendekatan nonprobability sampling atau teknik sampel jenuh yang dimana seluruh populasi menjadi sampel dalam penelitian ini. Hasil penelitan terlihat bahwa kepercayaan diri siswa kelas 8 SMPN 9 Bandung berada pada kategori sedang, siswa perempuan dan laki-laki tidak memiliki perbedaan yang signifikan. Program bimbingan dan konseling dibuat berdasarkan hasil penelitian yang telah dilakukan mengenai kepercayaan diri.
Penggunaan Teknik Bibliokonseling Berbantuan Happines In Jar pada Siswa yang Mengalami Afeksi Negatif
Authors: Tiara Tiara Anggun Mauladi , Sahril Buchori  , Akhmad Harum
Published: February 2024

Penelitian ini menelaah terkait Penggunaan Teknik Bibliokonseling Berbantuan Happines in jar Pada Siswa Yang Mengalami Afeksi Negatif di SMP Negeri 30 Makassar. Dengan tujuan penelitian untuk Mengetahui penggunaan Teknik Bibliokonseling dengan bantuan Happines in jar dapat mereduksi afeksi negatif siswa di SMP Negeri 30 Makassar. Adapun Metode yang digunakan dalam penelitian ini adalah Kuantitatif dengan jenis penelitian Quasi Eksperimen, dengan desain non-equivalent control group desain. Populasi dalam penelitian ini sebanyak 17 siswa yang terindifikasi mengalami Afeksi negatif yang tinggi, sehingga sampel dalam penelitian ini sebanyak 12 orang siswa dengan penentuan sampel menggunakan Teknik proportional random sampling. Teknik pengumpulan data yang digunakan yaitu angket, Observasi, dan dokumentasi. Analisis data menggunakan analisis deskriptif dan analisis statistic inferensial menggunakan SPSS v.25. Hasil Penelitian menunjukkan bahwa: Penggunaan Teknik bibliokonseling dengan bantuan bantuan Happines in jar dapat mereduksi afeksi negatif siswa di SMP Negeri 30 Makassar, hal ini dapat dilihat dari hasil perhitungan melalui uji independent samples t test diperoleh nilai sig. (2- tailed) =0.019 yang berarti lebih kecil dari <0,05 dengan demikian H0 ditolak dan H1 diterima. Sehingga dapat disimpulkan bahwa Teknik Bibliokonseling dengan bantuan bantuan Happines in jar dapat mereduksi afeksi negatif siswa siswa di SMP Negeri 30 Makassar.
Variations of Coconut Shell, Rice Husk, and Bagasse as an Alternative of Briquettes Materials
Authors: Sari Kartika , Anisyah Anisyah , Toruan Parmin Lumban , Setiawan Andi Arif
Published: February 2024

The current energy condition is getting thinner meanwhile people's daily lives still use a lot of energy derived from fossil fuels so that its use is increasing with limited availability. An alternative to reduce the use of fossil energy is by utilizing waste biomass as an energy source one of them is briquetts. This study aims to analyze the characteristics of briquettes from a variety of briquette mixtures made from coconut shells, rice husks, and bagasse. The method used is survey and experimental. The survey of sampling coconut shells was carried out in the Seberang Ulu I area, rice husks in the Pegayut area, and bagasse was obtained at the ice cane traders in the Seberang Ulu I area. The data obtained were presented in graphical form and analyzed descriptively. The results obtained are the water content in the variation of the combination of briquette-making materials ranging from 4.23 - 5.13%, ash content of 7.6 - 7.86%, the density value obtained is 0.55 - 0.59 g/cm3, the compressive strength value is 25.67 - 37.33 kg/cm2, and the calorific value is 4756 - 4865 cal/gr. The results showed there is not significant differences in moisture content, ash content, and compressive strength, while the density and calorific value showed significant differences.