bluuebunny/crossref_metadata_2025 huggingface dataset:
How the Ethylene Biosynthesis Pathway of Semi-Halophytes Is Modified with Prolonged Salinity Stress Occurrence?
Authors: Gieniec Miron , Miszalski Zbigniew , Rozpądek Piotr , Jędrzejczyk Roman J. , Czernicka Małgorzata , Nosek Michał
Published: May 2024

The mechanism of ethylene (ET)–regulated salinity stress response remains largely unexplained, especially for semi-halophytes and halophytes. Here, we present the results of the multifaceted analysis of the model semi-halophyte Mesembryanthemum crystallinum L. (common ice plant) ET biosynthesis pathway key components’ response to prolonged (14 days) salinity stress. Transcriptomic analysis revealed that the expression of 3280 ice plant genes was altered during 14-day long salinity (0.4 M NaCl) stress. A thorough analysis of differentially expressed genes (DEGs) showed that the expression of genes involved in ET biosynthesis and perception (ET receptors), the abscisic acid (ABA) catabolic process, and photosynthetic apparatus was significantly modified with prolonged stressor presence. To some point this result was supported with the expression analysis of the transcript amount (qPCR) of key ET biosynthesis pathway genes, namely ACS6 (1-aminocyclopropane-1-carboxylate synthase) and ACO1 (1-aminocyclopropane-1-carboxylate oxidase) orthologs. However, the pronounced circadian rhythm observed in the expression of both genes in unaffected (control) plants was distorted and an evident downregulation of both orthologs’ was induced with prolonged salinity stress. The UPLC-MS analysis of the ET biosynthesis pathway rate-limiting semi-product, namely of 1-aminocyclopropane-1-carboxylic acid (ACC) content, confirmed the results assessed with molecular tools. The circadian rhythm of the ACC production of NaCl-treated semi-halophytes remained largely unaffected by the prolonged salinity stress episode. We speculate that the obtained results represent an image of the steady state established over the past 14 days, while during the first hours of the salinity stress response, the view could be completely different.
Fructosyl Amino Oxidase as a Therapeutic Enzyme in Age-Related Macular Degeneration
Authors: Delanghe Joris R. , Diana Di Mavungu Jose , Beerens Koen , Himpe Jonas , Bostan Nezahat , Speeckaert Marijn M. , Vrielinck Henk , Vral Anne , Van Den Broeke Caroline , Huizing Manon , Van Aken Elisabeth
Published: May 2024

Age-related macular degeneration (AMD) is an age-related disorder that is a global public health problem. The non-enzymatic Maillard reaction results in the formation of advanced glycation end products (AGEs). Accumulation of AGEs in drusen plays a key role in AMD. AGE-reducing drugs may contribute to the prevention and treatment of AGE-related disease. Fructosamine oxidase (FAOD) acts on fructosyl lysine and fructosyl valine. Based upon the published results of fructosamine 3-kinase (FN3K) and FAOD obtained in cataract and presbyopia, we studied ex vivo FAOD treatment as a non-invasive AMD therapy. On glycolaldehyde-treated porcine retinas, FAOD significantly reduced AGE autofluorescence (p = 0.001). FAOD treatment results in a breakdown of AGEs, as evidenced using UV fluorescence, near-infrared microspectroscopy on stained tissue sections of human retina, and gel permeation chromatography. Drusen are accumulations of AGEs that build up between Bruch’s membrane and the retinal pigment epithelium. On microscopy slides of human retina affected by AMD, a significant reduction in drusen surface to 45 ± 21% was observed following FAOD treatment. Enzymatic digestion followed by mass spectrometry of fructose- and glucose-based AGEs (produced in vitro) revealed a broader spectrum of substrates for FAOD, as compared to FN3K, including the following: fructosyllysine, carboxymethyllysine, carboxyethyllysine, and imidazolone. In contrast to FN3K digestion, agmatine (4-aminobutyl-guanidine) was formed following FAOD treatment in vitro. The present study highlights the therapeutic potential of FAOD in AMD by repairing glycation-induced damage.
Meibum Lipidomic Analysis in Evaporative Dry Eye Subjects
Authors: Garcia-Queiruga Jacobo , Pena-Verdeal Hugo , Sabucedo-Villamarin Belen , Paz-Tarrio Monica , Guitian-Fernandez Esteban , Garcia-Resua Carlos , Yebra-Pimentel Eva , Giraldez Maria J.
Published: May 2024

Meibomian Glands (MG) are sebaceous glands responsible for the production of meibum, the main component of the Tear Film Lipid Layer (TFLL). The TFLL facilitates the spread of the tear film over the ocular surface, provides stability and reduces tear evaporation. Alterations in meibum composition lead to different ocular alterations like Meibomian Gland Dysfunction (MGD) and subsequent Evaporative Dry Eye (EDE). The aim of the present study was to investigate the composition and abundance of meibum lipids and their relationship with eyelid margin abnormalities, lipid layer patterns and MG status. The study utilizes a lipidomic approach to identify and quantify lipids in meibum samples using an Elute UHPLC system. This system considered all four dimensions (mass/charge, retention time, ion mobility and intensity) to provide the accurate identification of lipid species. Samples were categorized as healthy or low/no signs of alteration (group 1) or severe signs of alteration or EDE/MGD (group 2). The current investigation found differences in Variable Importance in Projection lipid abundance between both groups for the MGD signs studied. Changes in meibum composition occur and are related to higher scores in eyelid margin hyperaemia, eyelid margin irregularity, MG orifice plugging, MG loss and lipid layer pattern.
The Role of TRP Channels in Sepsis and Colitis
Authors: Dvornikova Kristina A. , Platonova Olga N. , Bystrova Elena Y.
Published: May 2024

To date, several members of the transient receptor potential (TRP) channels which provide a wide array of roles have been found in the gastrointestinal tract (GI). The goal of earlier research was to comprehend the intricate signaling cascades that contribute to TRP channel activation as well as how these receptors’ activity affects other systems. Moreover, there is a large volume of published studies describing the role of TRP channels in a number of pathological disorders, including inflammatory bowel disease (IBD) and sepsis. Nevertheless, the generalizability of these results is subject to certain limitations. For instance, the study of IBD relies on various animal models and experimental methods, which are unable to precisely imitate the multifactorial chronic disease. The diverse pathophysiological mechanisms and unique susceptibility of animals may account for the inconsistency of the experimental data collected. The main purpose of this study was to conduct a comprehensive review and analysis of existing studies on transient receptor potential (TRP) channels implicating specific models of colitis and sepsis, with particular emphasis on their involvement in pathological disorders such as IBD and sepsis. Furthermore, the text endeavors to evaluate the generalizability of experimental findings, taking into consideration the limitations posed by animal models and experimental methodologies. Finally, we also provide an updated schematic of the most important and possible molecular signaling pathways associated with TRP channels in IBD and sepsis.
SIRT1 Serum Concentrations in Lipodystrophic Syndromes
Authors: Salvatori Luisa , Magno Silvia , Ceccarini Giovanni , Tozzi Rossella , Contini Savina , Pelosini Caterina , Santini Ferruccio , Gnessi Lucio , Mariani Stefania
Published: May 2024

Lipodystrophies (LDs) are rare, complex disorders of the adipose tissue characterized by selective fat loss, altered adipokine profile and metabolic impairment. Sirtuins (SIRTs) are class III NAD+-dependent histone deacetylases linked to fat metabolism. SIRT1 plays a critical role in metabolic health by deacetylating target proteins in tissue types including liver, muscle, and adipose. Circulating SIRT1 levels have been found to be reduced in obesity and increased in anorexia nervosa and patients experiencing weight loss. We evaluated circulating SIRT1 levels in relation to fat levels in 32 lipodystrophic patients affected by congenital or acquired LDs compared to non-LD subjects (24 with anorexia nervosa, 22 normal weight, and 24 with obesity). SIRT1 serum levels were higher in LDs than normal weight subjects (mean ± SEM 4.18 ± 0.48 vs. 2.59 ± 0.20 ng/mL) and subjects with obesity (1.7 ± 0.39 ng/mL), whereas they were close to those measured in anorexia nervosa (3.44 ± 0.46 ng/mL). Our findings show that within the LD group, there was no relationship between SIRT1 levels and the amount of body fat. The mechanisms responsible for secretion and regulation of SIRT1 in LD deserve further investigation.
DeepSub: Utilizing Deep Learning for Predicting the Number of Subunits in Homo-Oligomeric Protein Complexes
Authors: Deng Rui , Wu Ke , Lin Jiawei , Wang Dehang , Huang Yuanyuan , Li Yang , Shi Zhenkun , Zhang Zihan , Wang Zhiwen , Mao Zhitao , Liao Xiaoping , Ma Hongwu
Published: May 2024

The molecular weight (MW) of an enzyme is a critical parameter in enzyme-constrained models (ecModels). It is determined by two factors: the presence of subunits and the abundance of each subunit. Although the number of subunits (NS) can potentially be obtained from UniProt, this information is not readily available for most proteins. In this study, we addressed this gap by extracting and curating subunit information from the UniProt database to establish a robust benchmark dataset. Subsequently, we propose a novel model named DeepSub, which leverages the protein language model and Bi-directional Gated Recurrent Unit (GRU), to predict NS in homo-oligomers solely based on protein sequences. DeepSub demonstrates remarkable accuracy, achieving an accuracy rate as high as 0.967, surpassing the performance of QUEEN. To validate the effectiveness of DeepSub, we performed predictions for protein homo-oligomers that have been reported in the literature but are not documented in the UniProt database. Examples include homoserine dehydrogenase from Corynebacterium glutamicum, Matrilin-4 from Mus musculus and Homo sapiens, and the Multimerins protein family from M. musculus and H. sapiens. The predicted results align closely with the reported findings in the literature, underscoring the reliability and utility of DeepSub.
Carnosic Acid Inhibits Herpes Simplex Virus Replication by Suppressing Cellular ATP Synthesis
Authors: Horváth Georgina , Molnár Edit , Szabó Zoltán , Kecskeméti Gábor , Juhász László , Tallósy Szabolcs Péter , Nyári József , Bogdanov Anita , Somogyvári Ferenc , Endrész Valéria , Burián Katalin , Virok Dezső P.
Published: May 2024

Acquiring resistance against antiviral drugs is a significant problem in antimicrobial therapy. In order to identify novel antiviral compounds, the antiviral activity of eight plants indigenous to the southern region of Hungary against herpes simplex virus-2 (HSV-2) was investigated. The plant extracts and the plant compound carnosic acid were tested for their effectiveness on both the extracellular and intracellular forms of HSV-2 on Vero and HeLa cells. HSV-2 replication was measured by a direct quantitative PCR (qPCR). Among the tested plant extracts, Salvia rosmarinus (S. rosmarinus) exhibited a 90.46% reduction in HSV-2 replication at the 0.47 μg/mL concentration. Carnosic acid, a major antimicrobial compound found in rosemary, also demonstrated a significant dose-dependent inhibition of both extracellular and intracellular forms of HSV-2. The 90% inhibitory concentration (IC90) of carnosic acid was between 25 and 6.25 μg/mL. Proteomics and high-resolution respirometry showed that carnosic acid suppressed key ATP synthesis pathways such as glycolysis, citrate cycle, and oxidative phosphorylation. Inhibition of oxidative phosphorylation also suppressed HSV-2 replication up to 39.94-fold. These results indicate that the antiviral action of carnosic acid includes the inhibition of ATP generation by suppressing key energy production pathways. Carnosic acid holds promise as a potential novel antiviral agent against HSV-2.
Molecular Insight of Plants Response to Drought Stress: Perspectives and New Insights towards Food Security
Authors: Marques Isabel , Hu Honghong
Published: May 2024

In the face of climate-induced challenges, understanding the intricate molecular mechanisms underlying drought tolerance in plants has become imperative [...]
Predicting Transcription Factor Binding Sites with Deep Learning
Authors: Ghosh Nimisha , Santoni Daniele , Saha Indrajit , Felici Giovanni
Published: May 2024

Prediction of binding sites for transcription factors is important to understand how the latter regulate gene expression and how this regulation can be modulated for therapeutic purposes. A consistent number of references address this issue with different approaches, Machine Learning being one of the most successful. Nevertheless, we note that many such approaches fail to propose a robust and meaningful method to embed the genetic data under analysis. We try to overcome this problem by proposing a bidirectional transformer-based encoder, empowered by bidirectional long-short term memory layers and with a capsule layer responsible for the final prediction. To evaluate the efficiency of the proposed approach, we use benchmark ChIP-seq datasets of five cell lines available in the ENCODE repository (A549, GM12878, Hep-G2, H1-hESC, and Hela). The results show that the proposed method can predict TFBS within the five different cell lines very well; moreover, cross-cell predictions provide satisfactory results as well. Experiments conducted across cell lines are reinforced by the analysis of five additional lines used only to test the model trained using the others. The results confirm that prediction across cell lines remains very high, allowing an extensive cross-transcription factor analysis to be performed from which several indications of interest for molecular biology may be drawn.
Unraveling the Connection: Pain and Endoplasmic Reticulum Stress
Authors: Kawanaka Ryoko , Jin Hisayo , Aoe Tomohiko
Published: May 2024

Pain is a complex and multifaceted experience. Recent research has increasingly focused on the role of endoplasmic reticulum (ER) stress in the induction and modulation of pain. The ER is an essential organelle for cells and plays a key role in protein folding and calcium dynamics. Various pathological conditions, such as ischemia, hypoxia, toxic substances, and increased protein production, may disturb protein folding, causing an increase in misfolding proteins in the ER. Such an overload of the folding process leads to ER stress and causes the unfolded protein response (UPR), which increases folding capacity in the ER. Uncompensated ER stress impairs intracellular signaling and cell function, resulting in various diseases, such as diabetes and degenerative neurological diseases. ER stress may be a critical universal mechanism underlying human diseases. Pain sensations involve the central as well as peripheral nervous systems. Several preclinical studies indicate that ER stress in the nervous system is enhanced in various painful states, especially in neuropathic pain conditions. The purpose of this narrative review is to uncover the intricate relationship between ER stress and pain, exploring molecular pathways, implications for various pain conditions, and potential therapeutic strategies.
Sodium Bicarbonate Decreases Alcohol Consumption in Mice
Authors: Lin Jason , Rivadeneira Ana P. , Ye Yani , Ryu Clara , Parvin Shangrila , Jang Kyeongran , Garraway Sandra M. , Choi Inyeong
Published: May 2024

We previously reported that mice with low neuronal pH drink more alcohol, demonstrating the importance of pH for alcohol reward and motivation. In this study, we tested whether systemic pH affects alcohol consumption and if so, whether it occurs by changing the alcohol reward. C57BL/6J mice were given NaHCO3 to raise their blood pH, and the animals’ alcohol consumption was measured in the drinking-in-the-dark and two-bottle free choice paradigms. Alcohol consumption was also assessed after suppressing the bitterness of NaHCO3 with sucrose. Alcohol reward was evaluated using a conditioned place preference. In addition, taste sensitivity was assessed by determining quinine and sucrose preference. The results revealed that a pH increase by NaHCO3 caused mice to decrease their alcohol consumption. The decrease in high alcohol contents (20%) was significant and observed at different ages, as well as in both males and females. Alcohol consumption was also decreased after suppressing NaHCO3 bitterness. Oral gavage of NaHCO3 did not alter quinine and sucrose preference. In the conditioned place preference, NaHCO3-treated mice spent less time in the alcohol-injected chamber. Conclusively, the results show that raising systemic pH with NaHCO3 decreases alcohol consumption, as it decreases the alcohol reward value.
Failure Mechanism and Thermal Runaway in Batteries during Micro-Overcharge Aging at Different Temperatures
Authors: Zhang Zhizu , Ji Changwei , Wang Yanan
Published: May 2024

This paper provides insights into the four key behaviors and mechanisms of the aging to failure of batteries in micro-overcharge cycles at different temperatures, as well as the changes in thermal stability. The test results from a scanning electron microscope (SEM) and an energy-dispersive spectrometer (EDS) indicate that battery failure is primarily associated with the rupture of cathode materials, the fracturing and pulverization of electrode materials on the anode current collector, and the formation of lithium dendrites. Additionally, battery safety is influenced by environmental temperatures and the battery’s state of health (SOH), with failed batteries exhibiting the poorest stability and the highest mass loss rates. Under isothermal conditions, micro-overcharge leads to battery failure without thermal runaway. Thus, temperature stands out as the most influential factor in battery safety. These insights hold significant theoretical and practical value for the development of more precise and secure battery management systems.
Low-Cycle Fatigue Behavior and the Combined Cyclic Hardening Material Model of Plate-Shaped Zn-22Al Alloy for Seismic Dampers
Authors: Liu Zongcheng , Han Jianping , Yang Penghui
Published: May 2024

This study investigates the potential of the plate-shaped Zn-22 wt.% Al (Zn-22Al) alloy as an innovative energy dissipation material for seismic damping devices, since plate-shaped material is more suitable to fabricate large-scale devices for building structures. The research begins with the synthesis of Zn-22Al alloy, given its unavailability in the commercial market. Monotonic tensile tests and low-cycle fatigue tests are performed to analyze material properties and fatigue performance of plate-shaped specimens. Monotonic tensile curves and cyclic stress–strain curves, along with SEM micrographs for microstructure and fracture surface analysis, are acquired. The combined cyclic hardening material model is calibrated to facilitate finite element analysis. Experimental results reveal exceptional ductility in Zn-22Al alloy, achieving a fracture strain of 200.37% (1.11 fracture strain). Fatigue life ranges from 1126 to 189 cycles with increasing strain amplitude (±0.8% to ±2.5%), surpassing mild steel by at least 6 times. The cyclic strain–life relationships align well with the Basquin–Coffin–Manson relationship. The combined kinematic/isotropic hardening model in ABAQUS accurately predicts the hysteretic behavior of the material, showcasing the promising potential of Zn-22Al alloy for seismic damping applications.
Effects of Omega-3 Polyunsaturated Fatty Acids, Docosahexaenoic Acid and Eicosapentaenoic Acid, on Post-Surgical Complications in Surgical Trauma Patients: Mechanisms, Nutrition, and Challenges
Authors: Ouagueni Asma , Al-Zoubi Raed M. , Zarour Ahmad , Al-Ansari Abdulla , Bawadi Hiba
Published: May 2024

This paper aims to provide an in-depth review of the specific outcomes associated with omega-3 polyunsaturated fatty acids (PUFAs), focusing on their purported effects on post-surgical complications in trauma patients. A comprehensive investigation of omega-3 polyunsaturated fatty acids was conducted until February 2023 using the PubMed database. Surgical trauma is characterized by a disruption in immune response post surgery, known to induce systemic inflammation. Omega-3 PUFAs are believed to offer potential improvements in multiple post-surgical complications because of their anti-inflammatory and antioxidant properties. Inconsistent findings have emerged in the context of cardiac surgeries, with the route of administration playing a mediating role in these outcomes. The effects of omega-3 PUFAs on post-operative atrial fibrillation have exhibited variability across various studies. Omega-3 PUFAs have demonstrated positive effects in liver surgery outcomes and in patients with acute respiratory distress syndrome. Omega-3 is suggested to offer potential benefits, particularly in the perioperative care of patients undergoing traumatic procedures. Incorporating omega-3 in such cases is hypothesized to contribute to a reduction in certain surgical outcomes, such as hospitalization duration and length of stay in the intensive care unit. Therefore, comprehensive assessments of adverse effects can aid in identifying the presence of subtle or inconspicuous side effects associated with omega-3.
Insights into the Anti-Adipogenic and Anti-Inflammatory Potentialities of Probiotics against Obesity
Authors: Kober A. K. M. Humayun , Saha Sudeb , Ayyash Mutamed , Namai Fu , Nishiyama Keita , Yoda Kazutoyo , Villena Julio , Kitazawa Haruki
Published: May 2024

Functional foods with probiotics are safe and effective dietary supplements to improve overweight and obesity. Thus, altering the intestinal microflora may be an effective approach for controlling or preventing obesity. This review aims to summarize the experimental method used to study probiotics and obesity, and recent advances in probiotics against obesity. In particular, we focused on studies (in vitro and in vivo) that used probiotics to treat obesity and its associated comorbidities. Several in vitro and in vivo (animal and human clinical) studies conducted with different bacterial species/strains have reported that probiotics promote anti-obesity effects by suppressing the differentiation of pre-adipocytes through immune cell activation, maintaining the Th1/Th2 cytokine balance, altering the intestinal microbiota composition, reducing the lipid profile, and regulating energy metabolism. Most studies on probiotics and obesity have shown that probiotics are responsible for a notable reduction in weight gain and body mass index. It also increases the levels of anti-inflammatory adipokines and decreases those of pro-inflammatory adipokines in the blood, which are responsible for the regulation of glucose and fatty acid breakdown. Furthermore, probiotics effectively increase insulin sensitivity and decrease systemic inflammation. Taken together, the intestinal microbiota profile found in overweight individuals can be modified by probiotic supplementation which can create a promising environment for weight loss along enhancing levels of adiponectin and decreasing leptin, tumor necrosis factor (TNF)-α, interleukin (IL)-6, monocyte chemotactic protein (MCP)-1, and transforming growth factor (TGF)-β on human health.
Analysis of the Protective Effects of Rosa roxburghii-Fermented Juice on Lipopolysaccharide-Induced Acute Lung Injury in Mice through Network Pharmacology and Metabolomics
Authors: Chen Zhiyu , Zhang Shuo , Sun Xiaodong , Meng Duo , Lai Chencen , Zhang Min , Wang Pengjiao , Huang Xuncai , Gao Xiuli
Published: May 2024

Acute lung injury, a fatal condition characterized by a high mortality rate, necessitates urgent exploration of treatment modalities. Utilizing UHPLS-Q-Exactive Orbitrap/MS, our study scrutinized the active constituents present in Rosa roxburghii-fermented juice (RRFJ) while also assessing its protective efficacy against LPS-induced ALI in mice through lung histopathological analysis, cytokine profiling, and oxidative stress assessment. The protective mechanism of RRFJ against ALI in mice was elucidated utilizing metabolomics, network pharmacology, and molecular docking methodologies. Our experimental findings demonstrate that RRFJ markedly ameliorates pathological injuries in ALI-afflicted mice, mitigates systemic inflammation and oxidative stress, enhances energy metabolism, and restores dysregulated amino acid and arachidonic acid metabolic pathways. This study indicates that RRFJ can serve as a functional food for adjuvant treatment of ALI.
High Adherence to the Mediterranean Dietary Pattern Is Inversely Associated with Systemic Inflammation in Older but Not in Younger Brazilian Adults
Authors: Bastos Amália Almeida , Félix Paula Victória , Valentini Neto João , Rogero Marcelo Macedo , Fisberg Regina Mara , Yannakoulia Mary , Ribeiro Sandra Maria Lima
Published: May 2024

The Mediterranean dietary pattern (MPD) has shown promise in preventing low-grade systemic inflammation (LGSI). This study tested if a high adherence to the MDP by younger and older Brazilian adults is associated with lower LGSI and investigated which Mediterranean food components may contribute to these associations. We performed a secondary study on 2015 ISA-Nutrition (290 younger adults (20–59 years old) and 293 older adults (≥60 years old)), a cross-sectional population-based study in São Paulo, SP, Brazil. The adherence to the MDP was assessed using the Mediterranean Diet Score (MedDietScore), obtained from two non-consecutive 24 h dietary recalls (24HDRs). The LGSI score (from plasma CRP, TNF-α, and adiponectin) identified the inflammatory status. Linear regression models assessed the association between LGSI and the MedDietScore. In older adults only, a high adherence to the MDP signified an 11.5% lower LGSI score. Older adults, classified with high adherence to the MDP, differed by consuming lower meat intake and full-fat dairy. Between older adults, the intake of vegetables and olive oil was inversely associated with the levels of LGSI. Thus, among older adults, the intake of some specific Mediterranean food determined high adherence to the MDP and was associated with decreased LGSI.
Impact of Transgenerational Nutrition on Nonalcoholic Fatty Liver Disease Development: Interplay between Gut Microbiota, Epigenetics and Immunity
Authors: Tzeng Hong-Tai , Lee Wei-Chia
Published: May 2024

Nonalcoholic fatty liver disease (NAFLD) has emerged as the most prevalent pediatric liver disorder, primarily attributed to dietary shifts in recent years. NAFLD is characterized by the accumulation of lipid species in hepatocytes, leading to liver inflammation that can progress to steatohepatitis, fibrosis, and cirrhosis. Risk factors contributing to NAFLD encompass genetic variations and metabolic disorders such as obesity, diabetes, and insulin resistance. Moreover, transgenerational influences, resulting in an imbalance of gut microbial composition, epigenetic modifications, and dysregulated hepatic immune responses in offspring, play a pivotal role in pediatric NAFLD development. Maternal nutrition shapes the profile of microbiota-derived metabolites in offspring, exerting significant influence on immune system regulation and the development of metabolic syndrome in offspring. In this review, we summarize recent evidence elucidating the intricate interplay between gut microbiota, epigenetics, and immunity in fetuses exposed to maternal nutrition, and its impact on the onset of NAFLD in offspring. Furthermore, potential therapeutic strategies targeting this network are also discussed.
An Evaluation and Improvement of Microphysical Parameterization for a Heavy Rainfall Process during the Meiyu Season
Authors: Zhou Zhimin , Du Muyun , Hu Yang , Kang Zhaoping , Yu Rong , Guo Yinglian
Published: May 2024

The present study assesses the simulated precipitation and cloud properties using three microphysics schemes (Morrison, Thompson and MY) implemented in the Weather Research and Forecasting model. The precipitation, differential reflectivity (ZDR), specific differential phase (KDP) and mass-weighted mean diameter of raindrops (Dm) are compared with measurements from a heavy rainfall event that occurred on 27 June 2020 during the Integrative Monsoon Frontal Rainfall Experiment (IMFRE). The results indicate that all three microphysics schemes generally capture the characteristics of rainfall, ZDR, KDP and Dm, but tend to overestimate their intensity. To enhance the model performance, adjustments are made based on the MY scheme, which exhibited the best performance. Specifically, the overall coalescence and collision parameter (Ec) is reduced, which effectively decreases Dm and makes it more consistent with observations. Generally, reducing Ec leads to an increase in the simulated content (Qr) and number concentration (Nr) of raindrops across most time steps and altitudes. With a smaller Ec, the impact of microphysical processes on Nr and Qr varies with time and altitude. Generally, the autoconversion of droplets to raindrops primarily contributes to Nr, while the accretion of cloud droplets by raindrops plays a more significant role in increasing Qr. In this study, it is emphasized that even if the precipitation characteristics could be adequately reproduced, accurately simulating microphysical characteristics remains challenging and it still needs adjustments in the most physically based parameterizations to achieve more accurate simulation.
Brain Networks Modulation during Simple and Complex Gait: A “Mobile Brain/Body Imaging” Study
Authors: Bonassi Gaia , Zhao Mingqi , Samogin Jessica , Mantini Dante , Marchese Roberta , Contrino Luciano , Tognetti Paola , Putzolu Martina , Botta Alessandro , Pelosin Elisa , Avanzino Laura
Published: May 2024

Walking encompasses a complex interplay of neuromuscular coordination and cognitive processes. Disruptions in gait can impact personal independence and quality of life, especially among the elderly and neurodegenerative patients. While traditional biomechanical analyses and neuroimaging techniques have contributed to understanding gait control, they often lack the temporal resolution needed for rapid neural dynamics. This study employs a mobile brain/body imaging (MoBI) platform with high-density electroencephalography (hd-EEG) to explore event-related desynchronization and synchronization (ERD/ERS) during overground walking. Simultaneous to hdEEG, we recorded gait spatiotemporal parameters. Participants were asked to walk under usual walking and dual-task walking conditions. For data analysis, we extracted ERD/ERS in α, β, and γ bands from 17 selected regions of interest encompassing not only the sensorimotor cerebral network but also the cognitive and affective networks. A correlation analysis was performed between gait parameters and ERD/ERS intensities in different networks in the different phases of gait. Results showed that ERD/ERS modulations across gait phases in the α and β bands extended beyond the sensorimotor network, over the cognitive and limbic networks, and were more prominent in all networks during dual tasks with respect to usual walking. Correlation analyses showed that a stronger α ERS in the initial double-support phases correlates with shorter step length, emphasizing the role of attention in motor control. Additionally, β ERD/ERS in affective and cognitive networks during dual-task walking correlated with dual-task gait performance, suggesting compensatory mechanisms in complex tasks. This study advances our understanding of neural dynamics during overground walking, emphasizing the multidimensional nature of gait control involving cognitive and affective networks.
Design of High-Precision Driving Control System for Charge Management
Authors: Wang Yang , Lv Boyan , Yu Tao , Wang Longqi , Wang Zhi
Published: May 2024

Due to the interaction of accumulated charges on the surface of a test mass with the surrounding electric and magnetic fields, the performance of inertial sensors is affected, necessitating charge management for the test mass. Discharge technology based on Ultraviolet LEDs is internationally recognized as the optimal solution for charge management. Precision driving of Ultraviolet LEDs is considered a key technology in charge management. This paper presents the driving control system used for Ultraviolet LEDs, achieving precision pulse-width-modulation-type current output with controllable pulse width and amplitude. The system generates the pulse-width-controllable pulse voltage signal via analog pulse-width modulation, and subsequently regulates the amplitude of the PWM signal through range switching. To convert the voltage into the pulse-width-modulation-type driving current, the improved Howland current source is employed. The test results demonstrate that the driving control system can output controllable current in the range of 0.01 mA to 10 mA, with a minimum step of 0.01 mA. The accuracy of the current reaches 1%, the stability within 1 h is better than 1%, and the load regulation is better than 2%. The driving control system provides an important reference for the integration of charge management system and the precision drive control method for LEDs.
AI Concepts for System of Systems Dynamic Interoperability
Authors: Nilsson Jacob , Javed Saleha , Albertsson Kim , Delsing Jerker , Liwicki Marcus , Sandin Fredrik
Published: May 2024

Interoperability is a central problem in digitization and System of Systems (SoS) engineering, which concerns the capacity of systems to exchange information and cooperate. The task to dynamically establish interoperability between heterogeneous cyber-physical systems (CPSs) at run-time is a challenging problem. Different aspects of the interoperability problem have been studied in fields such as SoS, neural translation, and agent-based systems, but there are no unifying solutions beyond domain-specific standardization efforts. The problem is complicated by the uncertain and variable relations between physical processes and human-centric symbols, which result from, e.g., latent physical degrees of freedom, maintenance, re-configurations, and software updates. Therefore, we surveyed the literature for concepts and methods needed to automatically establish SoSs with purposeful CPS communication, focusing on machine learning and connecting approaches that are not integrated in the present literature. Here, we summarize recent developments relevant to the dynamic interoperability problem, such as representation learning for ontology alignment and inference on heterogeneous linked data; neural networks for transcoding of text and code; concept learning-based reasoning; and emergent communication. We find that there has been a recent interest in deep learning approaches to establishing communication under different assumptions about the environment, language, and nature of the communicating entities. Furthermore, we present examples of architectures and discuss open problems associated with artificial intelligence (AI)-enabled solutions in relation to SoS interoperability requirements. Although these developments open new avenues for research, there are still no examples that bridge the concepts necessary to establish dynamic interoperability in complex SoSs, and realistic testbeds are needed.
A Comparison between Powder Bed Fusion of Polyamide 12 and Aluminum Computer Numeric Control Machining: A Carbon Footprint and Energy Assessment
Authors: Sipert Samuel , Almeida Edna dos Santos , Silva Bruno Caetano dos Santos , Silva Neto Hamilton de Araújo , Oliveira André Souza , Juliano Diego Russo , Coelho Rodrigo Santiago
Published: May 2024

This study presents a comparative life cycle assessment (LCA) of two manufacturing scenarios for a camera housing: Powder Bed Fusion (PBF) using polyamide 12 (PA-12) and Computer Numeric Control (CNC) machining using aluminum, for a cradle-to-gate boundary. The selected impact categories were cumulative energy demand (CED) and global warming potential (GWP). The key findings indicate that the PA-12 PBF part outperformed the aluminum CNC machining one in terms of environmental and energy performance, showing a significant reduction of approximately 90% in equivalent carbon emissions and 84% in cumulative energy demand. Sensitivity analyses revealed that the PBF process was highly sensitive to changes in the proportion of virgin/recycled material for the printing process, variations in the life cycle inventory (LCI) data sources for PA-12 powder production, and changes in the transportation system for imported materials, as is the case for the main input in the process (PA-12 powder). Sensitivity analysis also showed less impact for the PBF camera housing even considering a lifespan of one-fifth that of the aluminum for the impact categories considered. However, it should be noted that this analysis did not include considerations for the usage and end-of-life phases, which may have significant contributions to the overall environmental impact.
Innovation-Based Strategic Roadmap for Economic Sustainability and Diversity in Hydrocarbon-Driven Economies: The Qatar Perspective
Authors: Al-Sulaiti Ahmed , Hamouda Abdel Magid , Al-Yafei Hussein , Abdella Galal M.
Published: May 2024

This research addresses the critical opportunities and challenges confronting economic sustainability for hydrocarbon-based economies. The primary objective is to advocate for a transformative shift towards diversified and knowledge-centric economic models to ensure long-term sustainability. The literature review exposes vulnerabilities in hydrocarbon-based economies. The study innovatively explores multifaceted dimensions of economic diversification, including governance issues, societal well-being, and the necessity for robust ecosystem frameworks. Utilizing a qualitative case study methodology, the research investigates Qatar’s initiatives, such as the Qatar National Vision 2030 and government programs, aiming to reduce dependence on hydrocarbons and foster innovation. Insightful semi-structured interviews provide nuanced perspectives on innovation in the hydrocarbon context with business and academic professionals. The primary data collection method involved the following distinct groups: five business professionals and eleven academic experts, representing eight outstanding local and external organizations. Key findings underscore a holistic view of innovation, associating it with practical solutions, adaptability, and transformative potential, showcasing diverse approaches ranging from business-centric to collaborative and user-centric methods. Identified challenges in the education system, the urgency for a matured innovation ecosystem, and opportunities in sustainable energy further enrich the study. Moreover, this research examines the challenges and mitigation strategies associated with economic diversification in hydrocarbon-driven economies, focusing on Qatar. The research recommends sustained efforts in economic diversification, educational reform, and technological integration for hydrocarbon-based economies. Policymakers, businesses, and academics can leverage these insights to navigate the complexities of resource dependency and ensure long-term viability. The commitment to addressing challenges faced by hydrocarbon-dependent nations remains crucial, with a dedication to fostering economic diversity, innovation, and educational excellence for a resilient and prosperous future.
Optimizing Integrated Water and Electrical Networks through a Holistic Water–Energy Nexus Approach
Authors: Elbalki Mennatalla , Shaaban Mostafa F. , Osman Ahmed , Pietrasanta Ariana , Kamil Mohammed , Ali Abdelfatah
Published: May 2024

As water and electrical networks cannot be entirely independent, a more integrated approach, the water–energy nexus (WEN), is developed. A WEN is the basis of a smart city where water and electrical networks are interconnected and integrated by implementing efficient management strategies. Accordingly, this study develops a dynamic co-optimization model for designing and operating an integrated power and water system. The proposed co-optimization model minimizes the total annual and operational costs of a micro-WEN system while capturing its optimum design values and operating conditions and meeting the demands of the electrical and water networks. Furthermore, this work presents a plan for transitioning from thermal desalination to reverse osmosis (RO) desalination in the United Arab Emirates (UAE). The key objective is to decouple electricity and water production, effectively tackling the issue of operating the UAE’s power plants at low efficiency during the winter while ensuring an adequate water supply to meet the growing demand. The results show that the co-optimization model provides a significant reduction in the total operational cost with the integration of photovoltaic energy and shifting to RO. Most importantly, the micro-WEN system is optimized over multiple timescales to reduce the computation effort and memory requirements.
Assessing Multilateral Development Bank ESG Safeguard Integration with International Sustainability Ratings
Authors: Rodríguez Estévez Damián , Arce Ruíz Rosa María
Published: May 2024

In an era where sustainability is paramount, this study critically assesses how multilateral development banks (MDBs) integrate internationally recognized sustainability indicators into their ESG safeguard policies. MDBs have historically incorporated policies to manage environmental and social risks in project financing; yet, protections against negative impacts in developing countries often remain insufficient. On the other hand, several infrastructure sustainability rating systems have been established around the world in recent decades due to economic growth and the importance of controlling environmental impacts associated with the construction sector. The purpose of this study was to analyze whether and how the indicators that these internationally recognized systems use to rate whether a project is sustainable are integrated into these safeguards by using several methodologies, including an analysis of existing documentation, a high-level matrix, and qualitative methods based on co-occurrences using specialized “atlas ti” software. The results show that MDBs’ coverage of financial, governance, and country risks lacks the sustainability focus found in these rating systems. Therefore, this study that concludes MDB safeguards must evolve, balancing comprehensive sustainability parameters and detailed management guidelines and addressing impacts beyond statutory frameworks to encourage stakeholder engagement for more sustainable infrastructure projects.
Development and Effectiveness of an Agro-Healing Program Utilizing Rural Resources to Relieve Stress in Adults
Authors: Park Kyoung-Hee , Park Sin-Ae
Published: May 2024

The purpose of this study was to develop an agro-healing program using rural resources for stressed adults and to measure its effectiveness. A one-day agro-healing program was developed using the resources of a care farm located in Cheongju, South Korea. A total of 37 adults in their 20s to 50s who were experiencing mild or more severe stress participated in the agro-healing program. Analysis of stress-related psychological and emotional indicators of the agro-healing program revealed that post-treatment stress, anxiety, and depression indicators of the healing program significantly decreased (p< 0.05). Furthermore, mood state, vitality, and resilience indicators increased significantly, which demonstrated effectiveness in improving the mental health of participants with mental health issues. A Pearson correlation analysis between each psychological and emotional variable after the program found that stress had a significant correlation with all variables. Subjective vitality had a full mediating effect on the relationship between stress and resilience. Finally, the one-day agro-healing program was found to help relieve symptoms in adults with stress.
Analysis of the Cooperation of a Compressor Heat Pump with a PV System
Authors: Tomczuk Krzysztof , Obstawski Paweł
Published: May 2024

The decarbonization of heating systems is one of the present political and legislative directions of the European Union and its Member States. The main activities concern the energy performance of buildings and energy efficiency. The mentioned UE directives are the basis for the financial support of high-emission fossil fuel thermal energy source replacement with emission-free ones, in particular heat pumps. Other aspects are the support of PV installations and the thermal insulation of buildings. 85% of EU buildings were built before 2000, and among those, 75% have poor energy performance. Therefore, a significant number of buildings have only high-temperature wall radiators, and this was a motivation to prepare this article. The main innovation of this research was a new theoretical design of a high-temperature heat pump based on ecological refrigerants. The presented solution allows wall radiators to receive a hot water supply with temperatures of up to 85 °C during external temperatures of up to −20 °C. Typical heat pumps do not have these kinds of parameters, so the authors decided to verify the possibility of operating this device in such a wide temperature range. Another important aspect was the analysis of PV support. Finally, this paper investigates the possibility of heating an energy-efficient house with the newly designed high-temperature heat pump. Depending on the location in Poland, i.e., Suwałki, Warsaw, and Wrocław, the total electric energy supplied to the compressors was 2538–3364 kWh. The energy provided by the PV to supply power to the compressors is 482–570 kWh. The achieved PV energy self-consumption is 16.9–19.0%. The Seasonal Coefficient of Performance (SCOP) of the heat pump is 1.825–2.038 without PV and 2.515–2.970 with PV.
Regulated Deficit Irrigation to Boost Processing Tomato Sustainability and Fruit Quality
Authors: Burato Andrea , Fusco Giovanna Marta , Pentangelo Alfonso , Nicastro Rosalinda , Modugno Anna Francesca , Scotto di Covella Fabio , Ronga Domenico , Carillo Petronia , Campi Pasquale , Parisi Mario
Published: May 2024

Improving water use efficiency is gaining relevance for the sustainability of agricultural practices. In semi-arid Mediterranean areas, recent studies highlighted that future climatic scenarios will be even more critical for crops, given the increase in water scarcity. In this context, the rationalization of irrigation water is necessary to sustain processing tomato (Solanum lycopersicum L.) yield and quality since this crop requires large volumes of water. The present research aimed to identify the effects of a regulated deficit irrigation (RDI) strategy on the environmental and economic sustainability and fruit technological and functional quality of the processing tomato crop in the Mediterranean area. A two-year, open-field experiment was carried out to compare full irrigation management (IRR, restoring 100% ETc) with an RDI strategy based on restoring 50% ETc when the first fruit cluster reached the typical size (BBCH 701 phenological stage, relative to Solanaceous fruits). Remarkable water saving (21.46%, average of the two years) was achieved under RDI without significant variations in total and marketable yield compared to the IRR regime. Consequently, improved economic water productivity (+23.17%) was observed, allowing enhanced processing tomato sustainability. The RDI strategy boosted the glucose content (+17.78%), soluble solids content (SSC, +10.17%), and dry matter of the fruits (+10.03 g%). Furthermore, a higher SSC-to-titratable acidity ratio (+15.47%) and a negative shift in fructose/glucose balance (−7.71%) were observed in RDI-treated plants. Higher levels of the drought stress markers proline (+38.99%) and total polyphenols (+20.58%) were detected in RDI- compared to IRR-irrigated tomato fruits. These findings suggested the RDI strategy as an effective and sustainable approach for increasing both water productivity and the fruit quality of the processing tomato crop under semi-arid Mediterranean climatic conditions.