bluuebunny/crossref_metadata_2025 huggingface dataset:
Interpolation of Small Datasets in the Sandstone Hydrocarbon Reservoirs, Case Study of the Sava Depression, Croatia
Authors: Malvić Tomislav , Ivšinović Josip , Velić Josipa , Rajić Rajna
Published: May 2019

The interpolation of small datasets is challenging problem regarding the selection of interpolation methods and type of datasets. Here, for such analysis, the analysed data was taken in two hydrocarbon fields (“A” and “B”), located in the western part of the Sava Depression (in Northern Croatia). The selected reservoirs “L” (in the “A” Field) and “K” (“B”) are of Lower Pontian (Upper Miocene) age and belong to the Kloštar-Ivanić Formation. Due to strong tectonics, there are numerous tectonic blocks, each sampled with only a few wells. We selected two variables for interpolation—reservoirs permeabilities and injected volumes of field water. The following interpolation methods are described, compared and applied: Nearest Neighbourhood, Natural Neighbour (for the first time in the Sava Depression) and Inverse Distance Weighting. The last one has been recommended as the most appropriate in this study. Also, the presented research can be repeated in similar clastic environments at the same level hydrocarbon of exploration.
Appetite, Anatomy, and Desire in Caroline Era Poetry and Theatre
Authors: Woltmann Suzy
Published: May 2019

The Caroline Era in early modern England was characterized by political instability and theological revolution. In response, there were ideological attempts to regulate desire and a distinct focus on the creation of a public persona. These crises led to anxieties about gender, performance, and the body. Literary production at this time was founded in political conflict and was both a response to and an escape from these events. In this article, I argue that early modern writers literarily regulate gender and bodies through the personal, political and theological happenings that they respond to within their work. John Donne’s metaphysical poetry and George Herbert’s The Temple express the anxiety of desire involved in the process of seeking God. These texts translate this through the language of eros, bodily sacrificial connection to the Divine Logos, and communion that focuses on digestion and inclusion in the corporeal universe as a means to achieve the divine moment. Similarly, John Ford’s The Broken Heart portrays anxieties about desire and appetite through female anatomization. The portrayals of desire, anxiety, and appetite in these texts (all first published in 1633) are representative of the historical, political, and religious ideological structures that informed their creation.
Ground Penetrating Radar Investigation of Corvin Castle (Castelul Corvinilor), Hunedoara, Romania
Authors: Morris Isabel , Cleary Julia , Gonciar Andre , Glisic Branko
Published: May 2019

Corvin Castle, located in Hunedoara County (Transylvania), is an important Romanian cultural site. Originally, a fort constructed in the 14th century, it was first converted into a castle by Ioan de Hunedoara in the 15th century, frequently changing owners (with significant construction in the 15th and 17th centuries) until it was abandoned in the mid-19th century. After undergoing various ill-fated reconstruction efforts in the late 19th century, the castle reopened in the 1950s when the Romanian government renewed its interest in cultural sites and undertook a series of sparsely-documented archaeological investigations and conservation projects. Presently, restoration efforts require renewed investigation of Corvin Castle’s construction and history. Ground penetrating radar (GPR) is a promising tool for investigating the construction phases of heritage structures like Corvin Castle, where invasive methods are inappropriate and extensive historical modification has left incomplete records. In 2017, a comprehensive GPR survey of the castle was conducted. The survey recognizes features mentioned in texts, discovers previously unknown constructions, locates areas of moisture ingress around the courtyard, and identifies the extent and composition of the building foundations. Information gained from these scans, especially combined with printed sources, is an asset in planning restoration efforts and understanding the effects of past modifications.
LEDs Combined with CHO Sources and CCC Priming PLB Regeneration of Phalaenopsis
Authors: Mehraj Hasan , Alam Md. Meskatul , Habiba Sultana Umma , Mehbub Hasan
Published: May 2019

Throughout this study, the objective was to determine the most effective carbohydrate (CHO) sources under different light-emitting diodes (LEDs), and the impact of chlorocholine chloride (CCC), for the in vitro regeneration of the protocom-like bodies (PLBs) in Phalaenopsis ‘Fmk02010’. We applied 15 LEDs combined with three CHO sources and five CCC concentrations in the study. Organogenesis of PLBs was very poor in maltose both for the number of PLBs and their fresh weight (FW) compared to media containing sucrose and trehalose. Sucrose was the best CHO source under the red-white (RW) LED for the in vitro organogenesis of PLBs (PLBs: 54.13; FW: 0.109 g), while trehalose was best under the blue-white (BW) LED (PLBs: 36.33, FW: 0.129 g). The red-blue-white (RBW)-trehalose combination generated a suitable number of PLBs (35.13) with the highest FW (0.167 g). CCC at 0.01, 0.1, and 1 mgL−1CCC had no effect on PLB formation or FW, but 10 mg L−1 reduced both. RW-sucrose, BW-trehalose, and RBW-trehalose were the best combinations for PLB organogenesis. The addition of low concentrations of CCC in the plant culture medium are unnecessary.
Seasonal Groundwater Recharge Characterization Using Time-Lapse Electrical Resistivity Tomography in the Thepkasattri Watershed on Phuket Island, Thailand
Authors: Tesfaldet Yacob T. , Puttiwongrak Avirut
Published: May 2019

Understanding the recharge mechanisms in the vadose zone is crucial to groundwater management and artificial recharge development. In this study, a systematic characterization of seasonal groundwater recharge was done using time-lapse electrical resistivity tomography (time-lapse ERT). The objective of this study was to characterize the seasonal groundwater recharge through the vadose zone and streams. A total of six electrical resistivity surveys in two locations were taken during the dry and rainy seasons using an advanced geosciences incorporated (AGI) SuperSting R2 resistivity meter in 2018. Then, time-lapse inversion was calculated using the dry season ERT as the base model and the rainy season ERTs as the monitoring datasets. The results showed a significant decrease in inverted resistivity from the dry season to the rainy season, which suggests rainwater infiltration through the vadose zone. Similarly, significant water level rise was observed in wells monitored during the survey indicating groundwater recharge. The time-lapse ERT showed, in one case, the Nang Dak stream and the unsaturated zones are the preferential groundwater recharge zones throughout the year; in another case, the Rieng stream is the groundwater discharge zone and the vadose zone is the preferential recharge zone. Finally, a simplified conceptual hydrogeological model representing the study area is presented to visualize the recharge mechanisms in the study area.
Maternal Occupational Oil Mist Exposure and Birth Defects, National Birth Defects Prevention Study, 1997–2011
Authors: Siegel Miriam , Rocheleau Carissa M. , Johnson Candice Y. , Waters Martha A. , Lawson Christina C. , Riehle-Colarusso Tiffany , Reefhuis Jennita ,   The National Birth Defects Prevention Study
Published: May 2019

Workers in various industries can be exposed to oil mists when oil-based fluids are aerosolized during work processes. Oil mists can be inhaled or deposited on the skin. Little research exists on the reproductive effects of oil mist exposure in pregnant workers. We aimed to investigate associations between occupational oil mist exposure in early pregnancy and a spectrum of birth defects using data from 22,011 case mothers and 8140 control mothers in the National Birth Defects Prevention Study. In total, 150 mothers were rated as exposed. Manufacturing jobs, particularly apparel manufacturing, comprised the largest groups of exposed mothers. Mothers of infants with septal heart defects (odds ratio (OR): 1.8, 95% confidence interval (CI): 1.0–3.3), and especially perimembranous ventricular septal defects (OR: 2.5, CI: 1.2–5.2), were more likely to be occupationally exposed to oil mists in early pregnancy than control mothers; and their rater-estimated cumulative exposure was more likely to be higher. This was the first U.S. study evaluating associations between oil mist exposure and a broad spectrum of birth defects. Our results are consistent with previous European studies, supporting a potential association between oil-based exposures and congenital heart defects. Further research is needed to evaluate the reproductive effects of occupational oil mist exposure.
Analysis of Mortality Change Rate from Temperature in Summer by Age, Occupation, Household Type, and Chronic Diseases in 229 Korean Municipalities from 2007–2016
Authors: Park Jongchul , Chae Yeora , Choi Seo Hyung
Published: May 2019

This study analyzed mortality change rate (MCR: daily change rate of mortality at a given temperature per average summer mortality) for 229 municipalities in Korea considering age, occupation, household type, chronic diseases, and regional temperature distribution. We found that the MCR for heat wave differs depending on socioeconomic factors and the temperature distribution in the region. The MCRs for the elderly (≥65 years of age), outdoor workers, one-person households, and chronic disease patients start to increase at lower temperatures and react more sensitively to temperature than others. For the socioeconomic factors considered in this study, occupation was found to be the most significant factor for the MCR differences (outdoor workers 1.17 and others 1.10 above 35 °C, p < 0.01). The MCRs of elderly outdoor workers increased consistently with temperature, while the MCRs of younger outdoor workers decreased at 33 °C, the heat wave warning level in Korea. The MCRs in lower temperature regions start to increase at 28 °C, whereas the MCRs start to increase at 30 °C in higher temperature regions. The results of this study suggest that heat wave policies should be based on contextualized impacts considering age, occupation, household type, chronic disease, and regional temperature distribution.
Assessment of Spatial Variability across Multiple Pollutants in Auckland, New Zealand
Authors: Longley Ian , Tunno Brett , Somervell Elizabeth , Edwards Sam , Olivares Gustavo , Gray Sally , Coulson Guy , Cambal Leah , Roper Courtney , Chubb Lauren , Clougherty Jane E.
Published: May 2019

Spatial saturation studies using source-specific chemical tracers are commonly used to examine intra-urban variation in exposures and source impacts, for epidemiology and policy purposes. Most such studies, however, has been performed in North America and Europe, with substantial regional combustion-source contributions. In contrast, Auckland, New Zealand, a large western city, is relatively isolated in the south Pacific, with minimal impact from long-range combustion sources. However, fluctuating wind patterns, complex terrain, and an adjacent major port complicate pollution patterns within the central business district (CBD). We monitored multiple pollutants (fine particulate matter (PM2.5), black carbon (BC), elemental composition, organic diesel tracers (polycyclic aromatic hydrocarbons (PAHs), hopanes, steranes), and nitrogen dioxide (NO2)) at 12 sites across the ~5 km2 CBD during autumn 2014, to capture spatial variation in traffic, diesel, and proximity to the port. PM2.5 concentrations varied 2.5-fold and NO2 concentrations 2.9-fold across the CBD, though constituents varied more dramatically. The highest-concentration constituent was sodium (Na), a distinct non-combustion-related tracer for sea salt (µ = 197.8 ng/m3 (SD = 163.1 ng/m3)). BC, often used as a diesel-emissions tracer, varied more than five-fold across sites. Vanadium (V), higher near the ports, varied more than 40-fold across sites. Concentrations of most combustion-related constituents were higher near heavy traffic, truck, or bus activity, and near the port. Wind speed modified absolute concentrations, and wind direction modified spatial patterns in concentrations (i.e., ports impacts were more notable with winds from the northeast).
Factors Associated with Knowledge, Attitude and Practices of Common Eye Diseases in General Population: A Multicenter Cross-Sectional Study from Pakistan
Authors: Zhao Mingyue , Gillani Ali Hassan , Amirul Islam Fakir Mohammad , Ji Wenjing , Hayat Khezar , Li Zongjie , Akbar Jamshaid , Ahmed Abu Bakar , Azam Ans , Masood Imran , Fang Yu
Published: May 2019

High prevalence of blindness coupled with low public awareness of eye diseases is a severe problem in Pakistan. The objective of this study was to evaluate the awareness, attitude and practices related to common ocular problems and eye care use among general population of Punjab, Pakistan. Data were collected cross-sectionally, using random selection of participants from five districts of Punjab, Pakistan. A pre-tested questionnaire was used to collect the data from January to March 2017. The ordinal data were converted to interval--based data by Rasch analysis, which is termed as “knowledge score” and ranges from −3 to +3. Descriptive statistics, linear regression, binary logistic regression and chi square analysis were performed to evaluate the data. Of the 2073 people contacted, 96.4% (2019) responded the interviewer. The mean (SD) age was 39.9 (11.4) years, majority were males (64.8%) and only 3.1% were illiterate. The majority (68.1%) of respondents were aware that blindness can be prevented and are least aware of age-related macular degradation (31.4%). Multivariate analysis to knowledge scores demonstrated that male gender (β = 0.53 95%CI = 0.39, 0.66, p < 0.001), higher education (β = 0.31 95%CI = 0.25, 0.37, p < 0.001) were significantly associated with knowledge score. Eighty-two percent of our respondents had a positive attitude towards health-seeking behavior and those who had adequate income on most of occasions were 1.82 (AOR = 1.82 95%CI = 1.26, 2.62 p = 0.001) times more prone towards health-seeking behavior than those who had insufficient income. Out of all respondents, 21.5% had an eye examination at least once a year and this frequency was significantly higher in lower age, females, high education and high Socio Economic Status level (p < 0.05). Among the general population of Pakistan, awareness about prevention of blindness and refractive error was optimum, while awareness of blindness causing eye conditions, like age-related macular degradation was low. Intensive improvements in the health literacy and public interventions—specifically in old age people, males and respondents with lower education—are urgently needed.
Proton Pump Inhibitors and Fracture Risk: A Review of Current Evidence and Mechanisms Involved
Authors: Thong Benjamin Ka Seng , Ima-Nirwana Soelaiman , Chin Kok-Yong
Published: May 2019

The number of patients with gastroesophageal problems taking proton pump inhibitors (PPIs) is increasing. Several studies suggested a possible association between PPIs and fracture risk, especially hip fractures, but the relationship remains contentious. This review aimed to investigate the longitudinal studies published in the last five years on the relationship between PPIs and fracture risk. The mechanism underlying this relationship was also explored. Overall, PPIs were positively associated with elevated fracture risk in multiple studies (n = 14), although some studies reported no significant relationship (n = 4). Increased gastrin production and hypochlorhydria are the two main mechanisms that affect bone remodeling, mineral absorption, and muscle strength, contributing to increased fracture risk among PPI users. As a conclusion, there is a potential relationship between PPIs and fracture risks. Therefore, patients on long-term PPI treatment should pay attention to bone health status and consider prophylaxis to decrease fracture risk.
Associations between Polypharmacy, Self-Rated Health, and Depression in African American Older Adults; Mediators and Moderators
Authors: Bazargan Mohsen , Smith James , Saqib Mohammed , Helmi Hamid , Assari Shervin
Published: May 2019

Background. Despite the prevalence of multimorbidity among African American (AA) older adults, little information exists on correlates of polypharmacy (using 5+ medications) in AA older adults. There is more information available regarding the link between polypharmacy and physical aspects of health than subjective ones. Aims. In a local sample of AA older adults in Los Angeles, this study investigated the association of polypharmacy with self-rated health (SRH) and depression. We also explored gender differences in these links. Methods. This community-based study was conducted in south Los Angeles. A total number of 708 AA older adults (age ≥ 55 years) were entered into this study. From this number, 253 were AA men and 455 were AA women. Polypharmacy was the independent variable. Self-rated health (SRH) and depression were the dependent variables. Age, educational attainment, financial difficulty (difficulty paying bills, etc.), and marital status were covariates. Gender was the moderator. Multimorbidity, measured as the number of chronic diseases (CDs), was the mediator. Logistic regressions were applied for data analysis. Results. Polypharmacy was associated with worse SRH and depression. Multimorbidity fully mediated the association between polypharmacy and depressive symptoms. Multimorbidity only partially mediated the association between polypharmacy and poor SRH. Gender moderated the association between polypharmacy and SRH, as polypharmacy was associated with poor SRH in women but not men. Gender did not alter the association between polypharmacy and depression. Conclusions. AA older women with polypharmacy experience worse SRH and depression, an association which is partially due to the underlying multimorbidity. There is a need for preventing inappropriate polypharmacy in AA older adults, particularly when addressing poor SRH and depression in AA older women with multimorbidity.
Feasibility of Ski Mountaineering for Patients Following a Total Knee Arthroplasty: A Descriptive Field Study
Authors: Haslinger Simon , Huber Daniela , Morawetz David , Blank Cornelia , Prossegger Johanna , Dünnwald Tobias , Koller Arnold , Fink Christian , Hartl Arnulf , Schobersberger Wolfgang
Published: May 2019

Background: Total knee arthroplasty (TKA) is socially relevant due to its high prevalence, high incidence and the affected population. A subpopulation of TKA patients exists that strives to be active and also return to sports after total joint replacement. In this context, a further group of TKA patients is interested in high-impact physical activities and want to proceed with such activities even after surgery. Focusing on winter sports, there is still a lack of evidence on whether ski mountaineering is feasible for this subgroup of patients. Therefore, this feasibility study examines the effects of moderate ski mountaineering on strength, balance, functional abilities and mental health in persons following a TKA. Methods: Eight patients (six males, two females; median age, 63 ± Interquartile range 9 years) with TKA were included in this study. The volunteers, who were pre-selected for a 7-day holiday in Sankt Johann (Tyrol, Austria), participated in five guided ski mountaineering tours. Statistical analyses of non-parametric longitudinal data were performed using analysis of variance. For gait parameters and the Feeling Scale, one-factor longitudinal models were used. Statistical significance was set at the level of p < 0.05. Results: A significant decrease in the S3-Check MFT stability index (p = 0.04), a significant increase in general well-being (p = 0.05), and a trend towards a decrease in general stress (p = 0.1) were detected, while all other parameters were unaffected. Conclusion: A 7-day recreational ski mountaineering holiday had no negative effects on ski-experienced patients with TKA and seemed to increase well-being. Further studies should focus on larger groups and use controlled designs. Additionally, long-term effects should be evaluated.
Factors Influencing the Mental Health Consequences of Climate Change in Canada
Authors: Hayes Katie , Berry Peter , Ebi Kristie L.
Published: May 2019

Climate change is increasing risks to the mental health of Canadians. Impacts from a changing climate may outstrip the ability of Canadians and their health-sustaining institutions to adapt effectively and could increase poor mental health outcomes, particularly amongst those most marginalized in society. A scoping review of literature published during 2000–2017 explored risks, impacts, and vulnerabilities related to climate change and mental health. In this commentary, the authors present a new assessment of evidence from this scoping review and highlight factors that influence the capacity to adapt to the mental health consequences of a changing climate. Findings from this assessment reveal eleven key factors that influence the capacity to adapt: social capital; sense of community; government assistance; access to resources; community preparedness; intersectoral/transdisciplinary collaboration; vulnerability and adaptation assessments; communication and outreach; mental health literacy; and culturally relevant resources. Attention to these factors by Canadian decision makers can support proactive and effective management of the mental health consequences of climate change.
Temporal Cross-Correlations between Ambient Air Pollutants and Seasonality of Tuberculosis: A Time-Series Analysis
Authors: Wang Hua , Tian Changwei , Wang Wenming , Luo Xiaoming
Published: May 2019

The associations between ambient air pollutants and tuberculosis seasonality are unclear. We assessed the temporal cross-correlations between ambient air pollutants and tuberculosis seasonality. Monthly tuberculosis incidence data and ambient air pollutants (PM2.5, PM10, carbon monoxide (CO), nitrogen dioxide (NO2), ozone (O3), sulfur dioxide (SO2)) and air quality index (AQI) from 2013 to 2017 in Shanghai were included. A cross-correlogram and generalized additive model were used. A 4-month delayed effect of PM2.5 (0.55), PM10 (0.52), SO2 (0.47), NO2 (0.40), CO (0.39), and AQI (0.45), and a 6-month delayed effect of O3 (−0.38) on the incidence of tuberculosis were found. The number of tuberculosis cases increased by 8%, 4%, 18%, and 14% for a 10 μg/m3 increment in PM2.5, PM10, SO2, and NO2; 4% for a 10 unit increment in AQI; 8% for a 0.1 mg/m3 increment in CO; and decreased by 4% for a 10 μg/m3 increment in O3. PM2.5 concentrations above 50 μg/m3, 70 μg/m3 for PM10, 16 μg/m3 for SO2, 47 μg/m3 for NO2, 0.85 mg/m3 for CO, and 85 for AQI, and O3 concentrations lower than 95 μg/m3 were positively associated with the incidence of tuberculosis. Ambient air pollutants were correlated with tuberculosis seasonality. However, this sort of study cannot prove causality.
How Can We Improve the Transformation Success Rate of Research Results in the Pharmaceutical Industry? The Game Theoretic Model of Technology Transfer Subjects
Authors: Sun Ziyuan , Wang Man , Zhang Weiwei , Li Yanli , Wang Dan , Dong Feng
Published: May 2019

University–industry technology transfer (UITT) plays an important role in the construction of the national pharmaceutical innovation system. The speculations of a faculty inventor may hinder the successful transfer of pharmaceutical research results. This paper divides the specific process of the transformation of pharmaceutical research results into two parts: (1) an evolutionary game between faculty inventors and universities; and (2) a Stackelberg game between faculty inventors and pharmaceutical companies. Further, we carry out numerical simulations to analyze the impact of transformation success rate, income distribution coefficient, and a faculty inventor’s future working years on the transformation of pharmaceutical research results. The findings indicated that whether a combination of action strategies of faculty inventors and universities can evolve to the optimal equilibrium is determined by many factors, such as the technological transaction price of the pharmaceutical company and the reward or the income obtained by the faculty inventor. The transformation success rate and the income distribution coefficient are the key factors that affect the faculty inventor’s will and the behavior of the pharmaceutical company. The conclusions of this paper contribute to the research on how we can improve the success rate of research results and avoid resource waste, and provide a decision-making reference for the management of pharmaceutical research results in universities.
Collaborative Action on Fetal Alcohol Spectrum Disorder Prevention: Principles for Enacting the Truth and Reconciliation Commission Call to Action #33
Authors: Wolfson Lindsay , Poole Nancy , Morton Ninomiya Melody , Rutman Deborah , Letendre Sherry , Winterhoff Toni , Finney Catherine , Carlson Elizabeth , Prouty Michelle , McFarlane Audrey , Ruttan Lia , Murphy Lisa , Stewart Carmen , Lawley Lisa , Rowan Tammy
Published: May 2019

The association between fetal alcohol spectrum disorder (FASD), residential schools and subsequent assimilatory policies in Canada is of such significance that it was included in the groundbreaking Truth and Reconciliation Commission of Canada’s Final Report through Call to Action #33, which focuses on collaboratively developing FASD prevention programs in Indigenous communities. A consensus statement with eight tenets for enacting Call to Action #33 was co-developed in May 2017 using a Two-Eyed Seeing approach during and after a meeting on Indigenous approaches to FASD prevention held in Canada. The consensus statement provides guidance for creating community-based, culture-led FASD prevention programs in Indigenous communities. The eight tenets reflect the diverse perspectives of Indigenous and non-Indigenous participants, are grounded in available research evidence, and align with Indigenous worldviews and wellness models. This paper uses the consensus statement and eight exemplary FASD prevention programs from Indigenous communities and organizations across Canada to highlight identity, culture, and relationships as central elements of FASD prevention in Indigenous communities. The consensus statement provides guidance for developing community- and culture-led FASD prevention programs and highlights the importance of Indigenous knowledge systems in developing and researching FASD prevention in, and with, Indigenous communities.
Does the Development of a High-Speed Railway Improve the Equalization of Medical and Health Services? Evidence from China
Authors: Yang Gangqiang , Ma Yuxi , Xue Yongyu , Meng Xia
Published: May 2019

Does the development of a high-speed railway (HSR) have a significant impact on the equalization of medical and health resources allocated among cities? Based on the panel data of 67 cities in China from 2007 to 2016, this paper investigates the direct and dynamic effects of HSR development on the equalization of medical and health services by using the difference-in-differences (DID) method. The empirical results show that an HSR connection significantly reduces the equalization level of medical and health services in cities and that the effect is larger for the period from the year of the connection to the second year. However, in the long term, HSR development improves the equalization level of medical and health services in cities. Heterogeneity tests show that the effect of the HSR connection shows an “N”-shaped trend under different city scales, the equalization level of medical resources in the largest cities benefit the most from HSR development, and the Eastern and Western regions of China are more sensitive to the HSR connection. While the allocation of medical resources is in the direction of equalization, the level of medical resources is significantly more equal with the HSR development in cities with stronger financial capacity and non-core cities. The analysis of other city characteristics provides policy recommendations for improving the public services delivery mode in China’s heterogeneous cities in terms of HSR development.
Exploring the Relationship between Urban Quiet Areas and Perceived Restorative Benefits
Authors: Payne Sarah R. , Bruce Neil
Published: May 2019

To help mitigate the adverse health impacts of environmental noise, European cities are recommended to identify urban quiet areas for preservation. Procedures for identifying urban quiet areas vary across cities and between countries, and little is known of the strength of the salutogenic (health-promoting) benefits they may provide. Taking a multi-site approach, this study examines the potential of three sites as urban quiet areas and their associated health benefits, particularly in relation to perceived restorative benefits. Across three cities in the United Kingdom, an urban garden, urban park, and an urban square had sound pressure levels measured. Responses from 151 visitors to these sites evaluated the place as quiet, calm, and tranquil, and assessed their experience of the place in terms of perceived sounds, its benefits, how it made them feel, and perceived restoration. Depending on the criteria used, the sites varied in their suitability as urban quiet areas, although all provided perceived health benefits. Relationships between sound levels (subjective and objective) and perceived restoration were not linear, with the type of sounds heard and other aspects of the place experience believed to affect the relationship. Building on this work, a future experimental approach based on the study sites is planned to manipulate the multiple variables involved. This will provide a clearer understanding of the relationship between urban quiet areas and perceived restorative benefits.
Measuring Socioeconomic Inequalities in Obesity among Korean Adults, 1998–2015
Authors: Hwang Jongnam , Lee Eun-Young , Lee Chung Gun
Published: May 2019

Obesity is a prominent global public health challenge as its prevalence has grown. Even though the increase in prevalence of obesity in Korea has been relatively low, it is expected to continually increase in the next several years, leading to social and economic burdens. This study aimed to assess socioeconomic inequalities in obesity among Korean adults. Using nationally representative survey datasets, the concentration index (CI) and decomposition of the CI were used to capture and quantify obesity-related inequalities from 1998 to 2015. The results suggested that pro-poor inequalities in obesity existed in Korea, indicating that obesity was more concentrated among individuals with lower income. In a gender-stratified model, obesity was more concentrated among women with lower income and men with higher income, showing that the trend and magnitude of inequalities in obesity each vary by gender. The decomposition approach revealed that, over the past 17 years, the main contributors to the existing inequalities were higher education and higher income levels. These findings suggest that comprehensive and multifaceted interventions at the local and national levels should be considered to address the identified income- and education-related barriers with respect to obesity among Korean adults.
Legumain Promotes Atherosclerotic Vascular Remodeling
Authors: Ozawa Nana , Sato Yuki , Mori Yukari , Masuda Hiroko , Yamane Mao , Yamamoto Yuka , Shirai Remina , Watanabe Rena , Sato Kengo , Mori Yusaku , Hirano Tsutomu , Watanabe Takuya
Published: May 2019

Legumain, a recently discovered cysteine protease, is increased in both carotid plaques and plasma of patients with carotid atherosclerosis. Legumain increases the migration of human monocytes and human umbilical vein endothelial cells (HUVECs). However, the causal relationship between legumain and atherosclerosis formation is not clear. We assessed the expression of legumain in aortic atheromatous plaques and after wire-injury-induced femoral artery neointimal thickening and investigated the effect of chronic legumain infusion on atherogenesis in Apoe−/− mice. We also investigated the associated cellular and molecular mechanisms in vitro, by assessing the effects of legumain on inflammatory responses in HUVECs and THP-1 monocyte-derived macrophages; macrophage foam cell formation; and migration, proliferation, and extracellular matrix protein expression in human aortic smooth muscle cells (HASMCs). Legumain was expressed at high levels in atheromatous plaques and wire injury-induced neointimal lesions in Apoe−/− mice. Legumain was also expressed abundantly in THP-1 monocytes, THP-1 monocyte-derived macrophages, HASMCs, and HUVECs. Legumain suppressed lipopolysaccharide-induced mRNA expression of vascular cell adhesion molecule-1 (VCAM1), but potentiated the expression of interleukin-6 (IL6) and E-selectin (SELE) in HUVECs. Legumain enhanced the inflammatory M1 phenotype and oxidized low-density lipoprotein-induced foam cell formation in macrophages. Legumain did not alter the proliferation or apoptosis of HASMCs, but it increased their migration. Moreover, legumain increased the expression of collagen-3, fibronectin, and elastin, but not collagen-1, in HASMCs. Chronic infusion of legumain into Apoe−/− mice potentiated the development of atherosclerotic lesions, accompanied by vascular remodeling, an increase in the number of macrophages and ASMCs, and increased collagen-3 expression in plaques. Our study provides the first evidence that legumain contributes to the induction of atherosclerotic vascular remodeling.
Dynamic Transcriptome Changes Related to Oil Accumulation in Developing Soybean Seeds
Authors: Yang Songnan , Miao Long , He Jianbo , Zhang Kai , Li Yan , Gai Junyi
Published: May 2019

Soybean is one of the most important oil crops in the world. Revealing the molecular basis and exploring key candidate genes for seed oil synthesis has great significance for soybean improvement. In this study, we found that oil accumulation rates and gene expression levels changed dynamically during soybean seed development. The expression levels of genes in metabolic pathways such as carbon fixation, photosynthesis, glycolysis, and fatty acid biosynthesis were significantly up-regulated during the rapid accumulation of oil in developing soybean seeds. Through weighted correlation network analysis, we identified six co-expression modules associated with soybean seed oil content and the pink module was the most positively correlated (r = 0.83, p = 7 × 10−4) network. Through the integration of differential expression and co-expression analysis, we predicted 124 candidate genes potentially affecting soybean seed oil content, including seven genes in lipid metabolism pathway, two genes involved in glycolysis, one gene in sucrose metabolism, and 12 genes belonged to transcription factors as well as other categories. Among these, three genes (GmABI3b, GmNFYA and GmFAD2-1B) have been shown to control oil and fatty acid content in soybean seeds, and other newly identified candidate genes would broaden our knowledge to understand the molecular basis for oil accumulation in soybean seeds.
Glomerular Hematuria: Cause or Consequence of Renal Inflammation?
Authors: Moreno Juan Antonio , Sevillano Ángel , Gutiérrez Eduardo , Guerrero-Hue Melania , Vázquez-Carballo Cristina , Yuste Claudia , Herencia Carmen , García-Caballero Cristina , Praga Manuel , Egido Jesús
Published: May 2019

Glomerular hematuria is a cardinal symptom of renal disease. Glomerular hematuria may be classified as microhematuria or macrohematuria according to the number of red blood cells in urine. Recent evidence suggests a pathological role of persistent glomerular microhematuria in the progression of renal disease. Moreover, gross hematuria, or macrohematuria, promotes acute kidney injury (AKI), with subsequent impairment of renal function in a high proportion of patients. In this pathological context, hemoglobin, heme, or iron released from red blood cells in the urinary space may cause direct tubular cell injury, oxidative stress, pro-inflammatory cytokine production, and further monocyte/macrophage recruitment. The aim of this manuscript is to review the role of glomerular hematuria in kidney injury, the role of inflammation as cause and consequence of glomerular hematuria, and to discuss novel therapies to combat hematuria.
Medical Treatment for Osteoporosis: From Molecular to Clinical Opinions
Authors: Chen Li-Ru , Ko Nai-Yu , Chen Kuo-Hu
Published: May 2019

Osteoporosis is a major concern all over the world. With aging, a gradual loss of bone mass results in osteopenia and osteoporosis. Heritable factors account for 60–80% of optimal bone mineralization. Modifiable factors, such as weight-bearing exercise, nutrition, body mass, and hormonal milieu, play an important role in the development of osteopenia and osteoporosis in adulthood. Currently, anti-resorptive agents, including estrogen, bisphosphonates, and selective estrogen receptor modulators (SERMs), are the drugs of choice for osteoporosis. Other treatments include parathyroid hormone (PTH) as well as the nutritional support of calcium and vitamin D. New treatments such as tissue-selective estrogen receptor complexes (TSECs) are currently in use too. This review, which is based on a systematic appraisal of the current literature, provides current molecular and genetic opinions on osteoporosis and its medical treatment. It offers evidence-based information to help researchers and clinicians with osteoporosis assessment. However, many issues regarding osteoporosis and its treatment remain unknown or controversial and warrant future investigation.
Transcription, Processing, and Decay of Mitochondrial RNA in Health and Disease
Authors: Barchiesi Arianna , Vascotto Carlo
Published: May 2019

Although the large majority of mitochondrial proteins are nuclear encoded, for their correct functioning mitochondria require the expression of 13 proteins, two rRNA, and 22 tRNA codified by mitochondrial DNA (mtDNA). Once transcribed, mitochondrial RNA (mtRNA) is processed, mito-ribosomes are assembled, and mtDNA-encoded proteins belonging to the respiratory chain are synthesized. These processes require the coordinated spatio-temporal action of several enzymes, and many different factors are involved in the regulation and control of protein synthesis and in the stability and turnover of mitochondrial RNA. In this review, we describe the essential steps of mitochondrial RNA synthesis, maturation, and degradation, the factors controlling these processes, and how the alteration of these processes is associated with human pathologies.
Earliest Mechanisms of Dopaminergic Neurons Sufferance in a Novel Slow Progressing Ex Vivo Model of Parkinson Disease in Rat Organotypic Cultures of Substantia Nigra
Authors: Dal Ben Matteo , Bongiovanni Rosario , Tuniz Simone , Fioriti Emanuela , Tiribelli Claudio , Moretti Rita , Gazzin Silvia
Published: May 2019

The current treatments of Parkinson disease (PD) are ineffective mainly due to the poor understanding of the early events causing the decline of dopaminergic neurons (DOPAn). To overcome this problem, slow progressively degenerating models of PD allowing the study of the pre-clinical phase are crucial. We recreated in a short ex vivo time scale (96 h) all the features of human PD (needing dozens of years) by challenging organotypic culture of rat substantia nigra with low doses of rotenone. Thus, taking advantage of the existent knowledge, the model was used to perform a time-dependent comparative study of the principal possible causative molecular mechanisms undergoing DOPAn demise. Alteration in the redox state and inflammation started at 3 h, preceding the reduction in DOPAn number (pre-diagnosis phase). The number of DOPAn declined to levels compatible with diagnosis only at 12 h. The decline was accompanied by a persistent inflammation and redox imbalance. Significant microglia activation, apoptosis, a reduction in dopamine vesicle transporters, and the ubiquitination of misfolded protein clearance pathways were late (96 h, consequential) events. The work suggests inflammation and redox imbalance as simultaneous early mechanisms undergoing DOPAn sufferance, to be targeted for a causative treatment aimed to stop/delay PD.
C1 Compound Biosensors: Design, Functional Study, and Applications
Authors: Lee Jin-Young , Sung Bong Hyun , Oh So-Hyung , Kwon Kil Koang , Lee Hyewon , Kim Haseong , Lee Dae-Hee , Yeom Soo-Jin , Lee Seung-Goo
Published: May 2019

The microbial assimilation of one-carbon (C1) gases is a topic of interest, given that products developed using this pathway have the potential to act as promising substrates for the synthesis of valuable chemicals via enzymatic oxidation or C–C bonding. Despite extensive studies on C1 gas assimilation pathways, their key enzymes have yet to be subjected to high-throughput evolution studies on account of the lack of an efficient analytical tool for C1 metabolites. To address this challenging issue, we attempted to establish a fine-tuned single-cell–level biosensor system constituting a combination of transcription factors (TFs) and several C1-converting enzymes that convert target compounds to the ligand of a TF. This enzymatic conversion broadens the detection range of ligands by the genetic biosensor systems. In this study, we presented new genetic enzyme screening systems (GESSs) to detect formate, formaldehyde, and methanol from specific enzyme activities and pathways, named FA-GESS, Frm-GESS, and MeOH-GESS, respectively. All the biosensors displayed linear responses to their respective C1 molecules, namely, formate (1.0–250 mM), formaldehyde (1.0–50 μM), and methanol (5–400 mM), and they did so with high specificity. Consequently, the helper enzymes, including formaldehyde dehydrogenase and methanol dehydrogenase, were successfully combined to constitute new versatile combinations of the C1-biosensors.
Lysosome Alterations in the Human Epithelial Cell Line HaCaT and Skin Specimens: Relevance to Psoriasis
Authors: Bocheńska Katarzyna , Moskot Marta , Malinowska Marcelina , Jakóbkiewicz-Banecka Joanna , Szczerkowska-Dobosz Aneta , Purzycka-Bohdan Dorota , Pleńkowska Joanna , Słomiński Bartosz , Gabig-Cimińska Magdalena
Published: May 2019

Despite the constantly updated knowledge regarding the alterations occurring in the cells of patients with psoriasis, the status and the role of the lysosome, a control center of cell metabolism, remain to be elucidated. The architecture of the epidermis is largely regulated by the action of lysosomes, possibly activating signaling pathways in the cellular crosstalk of keratinocytes—epidermal cells—with infiltrating immune cells. Thus, in the present study, lysosome alterations were examined in vitro and in situ using a two-dimensional (2D) keratinocyte model of HaCaT cells with “psoriasis-like” inflammation and skin specimens, respectively. Specific fluorescence and immunohistochemical staining showed an augmented level of acidic organelles in response to keratinocyte activation (mimicking a psoriatic condition while maintaining the membrane integrity of these structures) as compared with the control, similar to that seen in skin samples taken from patients. Interestingly, patients with the most pronounced PASI (Psoriasis Area and Severity Index), BSA (Body Surface Area), and DLQI (Dermatology Life Quality Index) scores suffered a high incidence of positive lysosomal-associated membrane protein 1 (LAMP1) expression. Moreover, it was found that the gene deregulation pattern was comparable in lesioned (PP) and non-lesioned (PN) patient-derived skin tissue, which may indicate that these alterations occur prior to the onset of the characteristic phenotype of the disease. Changes in the activity of genes encoding the microphthalmia family (MiT family) of transcription factors and mammalian target of rapamycin complex 1 (MTORC1) were also observed in the in vitro psoriasis model, indicating that the biogenesis pathway of this arm is inhibited. Interestingly, in contrast to the keratinocytes of HaCaT with “psoriasis-like” inflammation, LAMP1 was up-regulated in both PP and PN skin, which can be a potential sign of an alternative mechanism of lysosome formation. Defining the molecular profile of psoriasis in the context of “the awesome lysosome” is not only interesting, but also desired; therefore, it is believed that this paper will serve to encourage other researchers to conduct further studies on this subject.
IL-1β Damages Fibrocartilage and Upregulates MMP-13 Expression in Fibrochondrocytes in the Condyle of the Temporomandibular Joint
Authors: Tabeian Hessam , Betti Beatriz F. , dos Santos Cirqueira Cinthya , de Vries Teun J. , Lobbezoo Frank , ter Linde Anouk V. , Zandieh-Doulabi Behrouz , Koenders Marije I. , Everts Vincent , Bakker Astrid D.
Published: May 2019

The temporomandibular joint (TMJ), which differs anatomically and biochemically from hyaline cartilage-covered joints, is an under-recognized joint in arthritic disease, even though TMJ damage can have deleterious effects on physical appearance, pain and function. Here, we analyzed the effect of IL-1β, a cytokine highly expressed in arthritic joints, on TMJ fibrocartilage-derived cells, and we investigated the modulatory effect of mechanical loading on IL-1β-induced expression of catabolic enzymes. TMJ cartilage degradation was analyzed in 8–11-week-old mice deficient for IL-1 receptor antagonist (IL-1RA−/−) and wild-type controls. Cells were isolated from the juvenile porcine condyle, fossa, and disc, grown in agarose gels, and subjected to IL-1β (0.1–10 ng/mL) for 6 or 24 h. Expression of catabolic enzymes (ADAMTS and MMPs) was quantified by RT-qPCR and immunohistochemistry. Porcine condylar cells were stimulated with IL-1β for 12 h with IL-1β, followed by 8 h of 6% dynamic mechanical (tensile) strain, and gene expression of MMPs was quantified. Early signs of condylar cartilage damage were apparent in IL-1RA−/− mice. In porcine cells, IL-1β strongly increased expression of the aggrecanases ADAMTS4 and ADAMTS5 by fibrochondrocytes from the fossa (13-fold and 7-fold) and enhanced the number of MMP-13 protein-expressing condylar cells (8-fold). Mechanical loading significantly lowered (3-fold) IL-1β-induced MMP-13 gene expression by condylar fibrochondrocytes. IL-1β induces TMJ condylar cartilage damage, possibly by enhancing MMP-13 production. Mechanical loading reduces IL-1β-induced MMP-13 gene expression, suggesting that mechanical stimuli may prevent cartilage damage of the TMJ in arthritic patients.