bluuebunny/crossref_metadata_2025 huggingface dataset:
Insecticidal Potential of Essential Oils from Ammi visnaga L. and Trachyspermum ammi L. against Sitophilus oryzae (L.) and In Silico Study of Their Major Constituents
Authors: Harmouzi Anjoud , EL Ammari Yassine , Mssillou Ibrahim , Chlouchi Amina , Lim Adrian , Abdelaziz Shahat Abdelaaty , Chebaibi Mohamed
Published: July 2024

There is a high interest in utilizing natural bioactive products derived from plants as a substitute for synthetic chemicals in the industry. This research focuses on the phytochemical composition of essential oils (EOs) of Ammi visnaga L. and Trachyspermum ammi L and their insecticidal activity against Sitophilus oryzae (L.), a common pest found in stored cereals. The EOs were extracted through steam distillation and analyzed using gas chromatography coupled with mass spectrometry (GC-MS). The EOs of A. visnaga consisted of twenty-four components, with Abietadiene (41.23%) being the most abundant, followed by linalool (25.54%) and limonene (19.04%). On the other hand, the EOs of T. ammi consisted of twenty-eight main components, with isothymol being the most abundant (51.88%). The results revealed that the EOs of T. Ammi (DL50 = 0.1 µL EOs/L of air) were more toxic than A. visnaga (0.38 µL EOs/L of air), with the toxicity varying based on doses and exposure periods. To further understand the molecular mechanisms underlying this activity, molecular docking and dynamic simulations were performed using the major chemical constituents of the oils. The simulation results indicated that the major compounds, Abietadiene and isothymol, interact with the catalytic sites of the target proteins, inhibiting acetylcholinesterase and chitin synthase. These interactions form energetically favorable systems that remain stable throughout the molecular dynamic period. This research provides valuable insights into the potential of these EOs as natural insecticides and highlights the importance of molecular modeling in understanding the biological activities of plant-derived compounds.
PdS-ZnS-Doped Electrospun Polymer Nanofibers as Effective Photocatalyst for Hydrogen Evolution
Authors: Panthi Gopal , Gyawali Arun
Published: July 2024

Poly(vinyl acetate) nanofibers doped with PdS-ZnS nanoparticles (PdS-ZnS/PVAc nanofibers) were fabricated via an electrospinning technique. PdS-ZnS nanoparticles were in situ synthesized by adding (NH4)2S solution to poly(vinyl acetate)/zinc acetate/palladium acetate solution. Electrospinning of the formed colloidal solution led to the formation of poly(vinyl acetate) nanofibers containing uniformly distributed PdS-ZnS nanoparticles. The prepared samples were characterized by field emission scanning electron microscopy, X-ray diffraction, transmission electron microscopy and Fourier transform infrared spectroscopy. In photocatalytic activity investigation, the PdS-ZnS/PVAc nanofibers showed remarkably enhanced performance towards water photosplitting under solar irradiation compared to the ZnS/PVAc nanofibers. This enhanced performance is attributed to the synergistic effects of heterostructured PdS-ZnS nanoparticles, which can improve photogenerated charge migration and solar light absorption.
Understanding Factors Influencing Diabetic Patients’ Intention to Use Digital Health Services in Saudi Arabia Using the Technology Acceptance Model
Authors: Al-Sulimani Tagreed Sadeek , Bouaguel Waad
Published: July 2024

This study employs the Technology Acceptance Model to investigate the factors influencing Saudi Arabian diabetic patients’ intention to use digital health services. There is an urgent need to investigate the possibilities of digital health services in managing diabetes given the startlingly rapidly increasing prevalence rate of diabetes in KSA. The study examines the variables affecting patients’ acceptance and desire to use digital health tools to manage their diabetes. The study employs the Technology Acceptance Model to ascertain the crucial factors that impact patients’ opinions regarding the usefulness and ease of use of digital healthcare technologies. The proposed model extends the traditional Technology Acceptance Model by adding two new constructs, perceived privacy and trust. These constructs were examined by analyzing the intentions of 600 respondents through online surveys. The study’s conclusions showed that attitudes toward using digital health services for KSA diabetic patients are greatly influenced by every component of the extended Technology Acceptance Model. The study’s conclusions add to the body of knowledge already in existence and offer insightful information to decision-makers hoping to improve digital health services.
Urtica dioica: Anticancer Properties and Other Systemic Health Benefits from In Vitro to Clinical Trials
Authors: Abi Sleiman Marc , Younes Maria , Hajj Roy , Salameh Tommy , Abi Rached Samir , Abi Younes Rimane , Daoud Lynn , Doumiati Jean Louis , Frem Francesca , Ishak Ramza , Medawar Christopher , Naim Hassan Y. , Rizk Sandra
Published: July 2024

While conventional medicine has advanced in recent years, there are still concerns about its potential adverse reactions. The ethnopharmacological knowledge established over many centuries and the existence of a variety of metabolites have made medicinal plants, such as the stinging nettle plant, an invaluable resource for treating a wide range of health conditions, considering its minimal adverse effects on human health. The aim of this review is to highlight the therapeutic benefits and biological activities of the edible Urtica dioica (UD) plant with an emphasis on its selective chemo-preventive properties against various types of cancer, whereby we decipher the mechanism of action of UD on various cancers including prostate, breast, leukemia, and colon in addition to evaluating its antidiabetic, microbial, and inflammatory properties. We further highlight the systemic protective effects of UD on the liver, reproductive, excretory, cardiovascular, nervous, and digestive systems. We present a critical assessment of the results obtained from in vitro and in vivo studies as well as clinical trials to highlight the gaps that require further exploration for future prospective studies.
Resveratrol and Vitamin D: Eclectic Molecules Promoting Mitochondrial Health in Sarcopenia
Authors: Russo Cristina , Valle Maria Stella , D’Angeli Floriana , Surdo Sofia , Malaguarnera Lucia
Published: July 2024

Sarcopenia refers to the progressive loss and atrophy of skeletal muscle function, often associated with aging or secondary to conditions involving systemic inflammation, oxidative stress, and mitochondrial dysfunction. Recent evidence indicates that skeletal muscle function is not only influenced by physical, environmental, and genetic factors but is also significantly impacted by nutritional deficiencies. Natural compounds with antioxidant properties, such as resveratrol and vitamin D, have shown promise in preventing mitochondrial dysfunction in skeletal muscle cells. These antioxidants can slow down muscle atrophy by regulating mitochondrial functions and neuromuscular junctions. This review provides an overview of the molecular mechanisms leading to skeletal muscle atrophy and summarizes recent advances in using resveratrol and vitamin D supplementation for its prevention and treatment. Understanding these molecular mechanisms and implementing combined interventions can optimize treatment outcomes, ensure muscle function recovery, and improve the quality of life for patients.
Survival-Related Genes on Chromosomes 6 and 17 in Medulloblastoma
Authors: Vriend Jerry , Liu Xiao-Qing
Published: July 2024

Survival of Medulloblastoma (MB) depends on various factors, including the gene expression profiles of MB tumor tissues. In this study, we identified 967 MB survival-related genes (SRGs) using a gene expression dataset and the Cox proportional hazards regression model. Notably, the SRGs were over-represented on chromosomes 6 and 17, known for the abnormalities monosomy 6 and isochromosome 17 in MB. The most significant SRG was HMGA1 (high mobility group AT-hook 1) on chromosome 6, which is a known oncogene and a histone H1 competitor. High expression of HMGA1 was associated with worse survival, primarily in the Group 3γ subtype. The high expression of HMGA1 was unrelated to any known somatic copy number alteration. Most SRGs on chromosome 17p were associated with low expression in Group 4β, the MB subtype, with 93% deletion of 17p and 98% copy gain of 17q. GO enrichment analysis showed that both chromosomes 6 and 17 included SRGs related to telomere maintenance and provided a rationale for testing telomerase inhibitors in Group 3 MBs. We conclude that HMGA1, along with other SRGs on chromosomes 6 and 17, warrant further investigation as potential therapeutic targets in selected subgroups or subtypes of MB.
Functional Characterization of the Human BRCA1 ∆11 Splicing Isoforms in Yeast
Authors: Galli Alvaro , Bellè Francesca , Fargnoli Arcangelo , Caligo Maria Adelaide , Cervelli Tiziana
Published: July 2024

BRCA1, a crucial tumor suppressor gene, has several splicing isoforms, including Δ9–11, Δ11, and Δ11q, which lack exon 11, coding for significant portions of the protein. These isoforms are naturally present in both normal and cancerous cells, exhibiting altered activity compared to the full-length BRCA1. Despite this, the impact on cancer risk of the germline intronic variants promoting the exclusive expression of these Δ11 isoforms remains uncertain. Consequently, they are classified as variants of uncertain significance (VUS), posing challenges for traditional genetic classification methods due to their rarity and complexity. Our research utilizes a yeast-based functional assay, previously validated for assessing missense BRCA1 variants, to compare the activity of the Δ11 splicing isoforms with known pathogenic missense variants. This approach allows us to elucidate the functional implications of these isoforms and determine whether their exclusive expression could contribute to increased cancer risk. By doing so, we aim to provide insights into the pathogenic potential of intronic VUS-generating BRCA1 splicing isoforms and improve the classification of BRCA1 variants.
Sws2 Gene Positively Regulates Melanin Production in Plectropomus leopardus Skin via Direct Regulation of the Synthesis of Retinoic Acid
Authors: Yu Haoran , Chen Huapeng , Wang Xinxin , Zhang Yichun , Tan Yafang , Wang Lei , Sun Junlong , Luo Jian , Song Feibiao
Published: July 2024

Opsins are a class of transmembrane proteins encoded by opsin genes, and they play a variety of functional roles. Short wavelength-sensitive opsin 2 (sws2), one of the five classes of visual opsin genes, mainly senses blue light. Previous research has indicated that sws2 is essential for melanocyte formation in fish; however, its specific role in skin color differentiation remains to be elucidated. Here, we identified the sws2 gene in a prized reef-dwelling fish, Plectropomus leopardus. The full-length P. leopardus sws2 gene encodes a protein consisting of 351 amino acids, and exhibits substantial homology with other fish species. The expression of the sws2 gene was widespread across P. leopardus tissues, with high expression in eye and skin tissues. Through immunohistochemistry and in situ hybridization analyses, we discovered that the sws2 gene was primarily localized in the rod and cone cells of the retina, and epidermal cells of the skin. Furthermore, dsRNA interference was used for sws2 gene knockdown in living P. leopardus to elucidate its function in skin color differentiation. Black-color-related genes, melanin contents, and tyrosinase activity in the skin significantly decreased after sws2 knockdown (p < 0.05), but red-color-related genes and carotenoid and lutein contents significantly increased (p < 0.05). Retinoic acid injection produced the opposite results. Our results suggested that the sws2 gene influences P. leopardus skin color regulation by affecting vitamin synthesis and melanin-related gene expression levels. This study establishes a foundation for elucidating the molecular mechanisms by which sws2 regulates melanocyte formation in fish skin.
Immunotherapy in Breast Cancer
Authors: Dvir Kathrin , Giordano Sara , Leone Jose Pablo
Published: July 2024

Breast cancer is a disease encompassing a spectrum of molecular subtypes and clinical presentations, each with distinct prognostic implications and treatment responses. Breast cancer has traditionally been considered an immunologically “cold” tumor, unresponsive to immunotherapy. However, clinical trials in recent years have found immunotherapy to be an efficacious therapeutic option for select patients. Breast cancer is categorized into different subtypes ranging from the most common positive hormone receptor (HR+), human epidermal growth factor receptor 2 (HER2)—negative type, to less frequent HER2− positive breast cancer and triple-negative breast cancer (TNBC), highlighting the necessity for tailored treatment strategies aimed at maximizing patient outcomes. Despite notable progress in early detection and new therapeutic modalities, breast cancer remains the second leading cause of cancer death in the USA. Moreover, in recent decades, breast cancer incidence rates have been increasing, especially in women younger than the age of 50. This has prompted the exploration of new therapeutic approaches to address this trend, offering new therapeutic prospects for breast cancer patients. Immunotherapy is a class of therapeutic agents that has revolutionized the treatment landscape of many cancers, namely melanoma, lung cancer, and gastroesophageal cancers, amongst others. Though belatedly, immunotherapy has entered the treatment armamentarium of breast cancer, with the approval of pembrolizumab in combination with chemotherapy in triple-negative breast cancer (TNBC) in the neoadjuvant and advanced settings, thereby paving the path for further research and integration of immune checkpoint inhibitors in other subtypes of breast cancer. Trials exploring various combination therapies to harness the power of immunotherapy in symbiosis with various chemotherapeutic agents are ongoing in hopes of improving response rates and prolonging survival for breast cancer patients. Biomarkers and precise patient selection for the utilization of immunotherapy remain cardinal and are currently under investigation, with some biomarkers showing promise, such as Program Death Lignat-1 (PDL-1) Combined Positive Score, Tumor Mutation Burden (TMB), and Tumor Infiltrating Lymphocytes (TILs). This review will present the current landscape of immunotherapy, particularly checkpoint inhibitors, in different types of breast cancer.
Gonadotropin Activity during Early Folliculogenesis and Implications for Polycystic Ovarian Syndrome and Premature Ovarian Insufficiency: A Narrative Review
Authors: Longobardi Salvatore , Klinger Francesca Gioia , Zheng Wenjing , Campitiello Maria Rosaria , D’Hooghe Thomas , La Marca Antonio
Published: July 2024

Female fertility depends on the ovarian reserve of follicles, which is determined at birth. Primordial follicle development and oocyte maturation are regulated by multiple factors and pathways and classified into gonadotropin-independent and gonadotropin-dependent phases, according to the response to gonadotropins. Folliculogenesis has always been considered to be gonadotropin-dependent only from the antral stage, but evidence from the literature highlights the role of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) during early folliculogenesis with a potential role in the progression of the pool of primordial follicles. Hormonal and molecular pathway alterations during the very earliest stages of folliculogenesis may be the root cause of anovulation in polycystic ovary syndrome (PCOS) and in PCOS-like phenotypes related to antiepileptic treatment. Excessive induction of primordial follicle activation can also lead to premature ovarian insufficiency (POI), a condition characterized by menopause in women before 40 years of age. Future treatments aiming to suppress initial recruitment or prevent the growth of resting follicles could help in prolonging female fertility, especially in women with PCOS or POI. This review will briefly introduce the impact of gonadotropins on early folliculogenesis. We will discuss the influence of LH on ovarian reserve and its potential role in PCOS and POI infertility.
Orchestrating Stress Responses in Multiple Sclerosis: A Role for Astrocytic IFNγ Signaling
Authors: Habean Maria L. , Kaiser Kaitlin E. , Williams Jessica L.
Published: July 2024

Multiple sclerosis (MS) is an inflammatory and neurodegenerative disease that is characterized by the infiltration of peripheral immune cells into the central nervous system (CNS), secretion of inflammatory factors, demyelination, and axonal degeneration. Inflammatory mediators such as cytokines alter cellular function and activate resident CNS cells, including astrocytes. Notably, interferon (IFN)γ is a prominent pleiotropic cytokine involved in MS that contributes to disease pathogenesis. Astrocytes are dynamic cells that respond to changes in the cellular microenvironment and are highly responsive to many cytokines, including IFNγ. Throughout the course of MS, intrinsic cell stress is initiated in response to inflammation, which can impact the pathology. It is known that cell stress is pronounced during MS; however, the specific mechanisms relating IFNγ signaling to cell stress responses in astrocytes are still under investigation. This review will highlight the current literature regarding the impact of IFNγ signaling alone and in combination with other immune mediators on astrocyte synthesis of free oxygen radicals and cell death, and cover what is understood regarding astrocytic mitochondrial dysfunction and endoplasmic reticulum stress.
Natural Compounds for Preventing Age-Related Diseases and Cancers
Authors: Ki Mi-Ran , Youn Sol , Kim Dong Hyun , Pack Seung Pil
Published: July 2024

Aging is a multifaceted process influenced by hereditary factors, lifestyle, and environmental elements. As time progresses, the human body experiences degenerative changes in major functions. The external and internal signs of aging manifest in various ways, including skin dryness, wrinkles, musculoskeletal disorders, cardiovascular diseases, diabetes, neurodegenerative disorders, and cancer. Additionally, cancer, like aging, is a complex disease that arises from the accumulation of various genetic and epigenetic alterations. Circadian clock dysregulation has recently been identified as an important risk factor for aging and cancer development. Natural compounds and herbal medicines have gained significant attention for their potential in preventing age-related diseases and inhibiting cancer progression. These compounds demonstrate antioxidant, anti-inflammatory, anti-proliferative, pro-apoptotic, anti-metastatic, and anti-angiogenic effects as well as circadian clock regulation. This review explores age-related diseases, cancers, and the potential of specific natural compounds in targeting the key features of these conditions.
The Clinical Significance of CRNDE Gene Methylation, Polymorphisms, and CRNDEP Micropeptide Expression in Ovarian Tumors
Authors: Szafron Laura Aleksandra , Iwanicka-Nowicka Roksana , Podgorska Agnieszka , Bonna Arkadiusz M. , Sobiczewski Piotr , Kupryjanczyk Jolanta , Szafron Lukasz Michal
Published: July 2024

CRNDE is an oncogene expressed as a long non-coding RNA. However, our team previously reported that the CRNDE gene also encodes a micropeptide, CRNDEP. The amino acid sequence of CRNDEP has recently been revealed by other researchers, too. This study aimed to investigate genetic alterations within the CRNDEP-coding region of the CRNDE gene, methylation profiling of this gene, and CRNDEP expression analysis. All investigations were performed on clinical material from patients with ovarian tumors of diverse aggressiveness. We found that CRNDEP levels were significantly elevated in highly aggressive tumors compared to benign neoplasms. Consistently, a high level of this micropeptide was a negative, independent, prognostic, and predictive factor in high-grade ovarian cancer (hgOvCa) patients. The cancer-promoting role of CRNDE(P), shown in our recent study, was also supported by genetic and epigenetic results obtained herein, revealing no CRNDEP-disrupting mutations in any clinical sample. Moreover, in borderline ovarian tumors (BOTS), but not in ovarian cancers, the presence of a single nucleotide polymorphism in CRNDE, rs115515594, significantly increased the risk of recurrence. Consistently, in BOTS only, the same genetic variant was highly overrepresented compared to healthy individuals. We also discovered that hypomethylation of CRNDE is associated with increased aggressiveness of ovarian tumors. Accordingly, hypomethylation of this gene’s promoter/first exon correlated with hgOvCa resistance to chemotherapy, but only in specimens with accumulation of the TP53 tumor suppressor protein. Taken together, these results contribute to a better understanding of the role of CRNDE(P) in tumorigenesis and potentially may lead to improvements in screening, diagnosis, and treatment of ovarian neoplasms.
Transcriptional Silencing of 35S rDNA in Tragopogon porrifolius Correlates with Cytosine Methylation in Sequence-Specific Manner
Authors: Matyášek Roman , Kalfusová Radka , Kuderová Alena , Řehůřková Kateřina , Sochorová Jana , Kovařík Aleš
Published: July 2024

Despite the widely accepted involvement of DNA methylation in the regulation of rDNA transcription, the relative participation of different cytosine methylation pathways is currently described only for a few model plants. Using PacBio, Bisulfite, and RNA sequencing; PCR; Southern hybridizations; and FISH, the epigenetic consequences of rDNA copy number variation were estimated in two T. porrifolius lineages, por1 and por2, the latter with more than twice the rDNA copy numbers distributed approximately equally between NORs on chromosomes A and D. The lower rDNA content in por1 correlated with significantly reduced (>90%) sizes of both D-NORs. Moreover, two (L and S) prominent rDNA variants, differing in the repetitive organization of intergenic spacers, were detected in por2, while only the S-rDNA variant was detected in por1. Transcriptional activity of S-rDNA in por1 was associated with secondary constriction of both A-NORs. In contrast, silencing of S-rDNA in por2 was accompanied by condensation of A-NORs, secondary constriction on D-NORs, and L-rDNA transcriptional activity, suggesting (i) bidirectional nucleolar dominance and (ii) association of S-rDNAs with A-NORs and L-rDNAs with D-NORs in T. porrifolius. Each S- and L-rDNA array was formed of several sub-variants differentiating both genetically (specific SNPs) and epigenetically (transcriptional efficiency and cytosine methylation). The most significant correlations between rDNA silencing and methylation were detected for symmetric CWG motifs followed by CG motifs. No correlations were detected for external cytosine in CCGs or asymmetric CHHs, where methylation was rather position-dependent, particularly for AT-rich variants. We conclude that variations in rDNA copy numbers in plant diploids can be accompanied by prompt epigenetic responses to maintain an appropriate number of active rDNAs. The methylation dynamics of CWGs are likely to be the most responsible for regulating silent and active rDNA states.
A Comprehensive Review of Biologics in Phase III and IV Clinical Trials for Atopic Dermatitis
Authors: Waligóra-Dziwak Katarzyna , Dańczak-Pazdrowska Aleksandra , Jenerowicz Dorota
Published: July 2024

Atopic dermatitis (AD) is a skin condition characterized by significant challenges and a substantial deterioration in the life quality for affected patients. The therapeutic landscape for AD has witnessed a transformative shift with the emergence of biologic therapies. Our focus centers on biologics currently undergoing phase III and IV clinical trials, deeming them to hold the highest potential for significant clinical relevance. To identify biologic drugs under development in phase III and IV clinical trials, we searched ClinicalTrials.gov. Additional relevant trials were identified through JapicCTI/ Japan Registry of Clinical Trials (jRCT) with a citation search. A search in MEDLINE and EMBASE was performed. There have been 76 clinical trials identified concerning biologic drugs: dupilumab (34 trials), lebrikizumab (14 trials), tralokinumab (10 trials), rocatinlimab (7 trials), amlitelimab (2 trials), nemolizumab (6 trials), MG-K10 (1 trial), CM310 (1 trial), 611 (1 trial). A search in MEDLINE revealed 132 articles concerning phase III and IV clinical trials for AD treatment. A total of 39 articles concerned biologic drugs covering 23 clinical trials. A search in EMBASE revealed 268 relevant articles, allowing us to identify results of an additional six clinical trials. The safety and efficacy of these biologics are comprehensively addressed in this review. This comprehensive review aims to explore the current landscape of biologic therapies for AD, delving into the latest research findings, clinical trial outcomes, and the diverse mechanisms of action employed by these novel interventions.
Opportunities and Challenges in Cross-Country Collaboration: Insights from the Beneluxa Initiative
Authors: Claessens Zilke , Lammens Michiel , Barbier Liese , Huys Isabelle
Published: July 2024

National pricing and reimbursement agencies face growing challenges with complex health technologies, prompting European policy advancements. Beneluxa is a cross-country collaboration involving Belgium, the Netherlands, Luxemburg, Austria, and Ireland that aims to address sustainable access to medicines. In view of the soon-to-be-implemented EU HTA Regulation, insights and experiences from stakeholders with Beneluxa cross-country collaboration could provide possible transferable learnings. Therefore, this research aims to (i) identify the opportunities and challenges faced by Beneluxa, (ii) gather insights from stakeholders, namely (possible) applicants and policymakers, within and beyond Beneluxa on the initiative and broader cross-country collaboration principles, and (iii) transfer these insights into learnings and recommendations in anticipation of the full implementation of the new HTA Regulation. Fifteen semi-structured interviews were conducted with industry and European HTA/policy stakeholders. The principal challenges discussed by stakeholders encompass hesitancy from the industry toward Beneluxa assessments, which were attributed to procedural and timeline uncertainties, legislative framework ambiguity, and challenges in terms of industry’s internal organization. Another challenge highlighted is the resource-intensive nature of the procedure due to diverse approaches among member states. In addition, industry stakeholders mentioned limited communication and procedural complexity. Despite challenges, both stakeholder groups recognized important opportunities for cross-country collaboration. Transferable insights for future cross-country collaboration include transparent communication, clear legislative embedding, internal industry restructuring to facilitate joint HTAs, and member state support for conducting collaborative assessments. The study underscores diverging views among stakeholders on cross-country collaboration’s potential to support HTA and the market access of complex health technologies. While acknowledging benefits, there still are challenges, including industry hesitancy, emphasizing the need for transparent communication and clear guidance in the evolving EU HTA landscape.
A Workflow for the Compensation of Substrate Defects When Overprinting in Extrusion-Based Processes
Authors: Atzler Fynn , Hümbert Simon , Voggenreiter Heinz
Published: July 2024

Fused granular fabrication (FGF) is used in industrial applications to manufacture complex parts in a short time frame and with reduced costs. Recently, the overprinting of continuous fibre-reinforced laminates has been discussed to produce high-performance, functional structures. A hybrid process combining FGF with Automated Fibre Placement (AFP) was developed to implement this approach, where an additively manufactured structure is bonded in situ onto a thermoplastic laminate. However, this combination places great demands on process control, especially in the first printing layer. When 3D printing onto a laminate, the height of the first printed layer is decisive to the shear strength of the bonding. Manufacturing-induced surface defects of a laminate, like thermal warpage, gaps, and tape overlaps, can result in deviations from the ideal geometry and thus impair the bonding strength when left uncompensated. This study, therefore, proposes a novel process flow that uses a 3D scan of a laminate to adjust the geometry of the additively manufactured structure to achieve a constant layer height in the 3D print and, thus, constant mechanical properties. For the above-listed surface defects, only thermal warpage was found to have a significant effect on the bonding strength.
Outbreak of Ralstonia spp. and Burkholderia spp. Catheter-Related Bloodstream Infection in Hemodialysis Unit
Authors: Valente Mauro , Orecchioni Francesca , Brigante Fabiana , Moretti Maria Ilaria , Mariani Roberta , D’Errico Marcello Mario , Moretti Marco , Tavio Marcello , Ferreiro Cotorruelo Maria Soledad , Marchi Massimo , Moglie Emanuele , Ranghino Andrea
Published: July 2024

The Ralstonia species (RB) and Burkholderia species (BB) are bacteria responsible for nosocomial infections in frail patients such as hemodialyzed (HD) patients. Here, we report how we managed an outbreak caused by RB and BB that occurred in a dialysis unit. From the 7th to the 16th of April 2021, an infection due to RB and BB occurred in 7 out of 39 (17.9%) HD patients with central venous catheter (CVC). Disinfectants, CVC-lock therapy solutions, water by reverse osmosis unit (ROW) and dialysis concentrates were cultured, including the biofilm from the loading plastic tubes (LPTs) that connect the hemodialysis consoles (HCs) to the ROW delivery line. The antibiotic treatment was successful for all patients. RB and BB were isolated in the biofilm of 11/37 LPTs. Three out of 11 positive LPTs were associated with the infected patients. The ROW delivery line was modified to provide a whole disinfection with the HCs connected, avoiding the risk of new contamination of the LPTs. A filtration module of 0.01 mm was added prior to the ROW delivery line. Our experience suggests that outbreaks sustained by unusual bacteria such as RB and BB should be promptly investigated to treat the infected patients with the appropriate therapy and to identify the possible source of infection, making the needful changes to achieve a safer dialysis unit.
Semantic Fields and Castilianization in Galician: A Comparative Study with the Loanword Typology Project
Authors: Álvarez de la Granja María , Dubert García Francisco
Published: July 2024

This study examines the correspondence between the borrowability indices from the Loanwoard Typology (LWT) project and Castilianization indices from the Atlas Lingüístico Galego (ALGa) across seven semantic fields. To this end, we identified all Castilianisms in the ALGa and conducted a quantitative analysis to compare these indices. Results obtained indicate a mismatch between the rankings of the LWT project and the ALGa. For example, the field ‘The body’ has the highest level of Castilianization according to the ALGa but the lowest borrowed score in the LWT project. Moreover, Castilianization levels in the ALGa show greater dispersion than borrowability levels from the LWT project. In fact, in each semantic field, many concepts (52.2%) have low levels of Castilianization, between 0% and 10%, and only a few concepts have high levels. A more detailed analysis of three semantic fields (‘The body’, ‘Agriculture and vegetation’, and ‘The physical world’) suggests that explanations based solely on semantic criteria (such as the existence of an unalterable central lexicon) are insufficient; other factors such as prestige, urbanization, cultural modernity, frequency of word usage, and perhaps other intralinguistic factors should be taken into account.
Study of Volatile Organic Compounds in Emission from Bottom Sediments of Three Lakes with Impact of Anthropopression Using the Proton Transfer Reaction Mass Spectrometry
Authors: Antonowicz Józef , Wróblewski Tomasz
Published: July 2024

Studies of volatile organic compounds (VOCs) emitted from the bottom sediments of three Pomeranian lakes in Poland: Łazienkowskie, Rychnowskie, and Jeleń were conducted. All three lakes are subject to anthropogenic pressure but to varying degrees. In 2021, bottom sediment samples were taken from the lakes studied and an analysis of the emission of 20 volatile organic compounds was carried out using a proton transfer reaction mass spectrometer (PTR-MS). Concentrations in emissions from the bottom sediments of VOCs with the following mass–charge ratio (m/z) were analyzed: 57, 61, 63, 69, 75, 81, 83, 85, 87, 95, 97, 99, 101, 109, 111, 127, 129, 137, 149, and 157. The obtained data were analyzed by performing statistical tests and multivariate cluster and PCA analysis. The analysis shows that the lowest concentrations of VOCs were observed from bottom sediments in Lake Jeleń, which is subject to the lowest anthropopressure among the studied lakes. The analysis shows that the lowest concentrations of VOCs were observed from bottom sediments in Lake Jeleń, which is subject to lower anthropopressure among the studied lakes. With the help of cluster analysis, it was possible to collect data on the VOC concentrations into clusters, which resulted in demonstrating similarities between Łazienkowskie and Rychnowskie lakes—lakes connected by an isthmus, and the different characteristics of Lake Jeleń. PCA analysis leads to similar observations. The tested m/z VOCs can be identified using additional analytical methods.
Preparation of (Nd, Ce)-Fe-B Regenerated Magnets by In-Situ Restoration of Grain Boundary Structure Using Nascent Nd-Fe-B Powder
Authors: Yu Xi , Luo Sangen , Yang Munan , Shen Qingpeng , Yang Honglong , Zhong Shuwei , Zhang Weilong , Yang Bin
Published: July 2024

Rare earth resource recycling is an important endeavor for environmental protection and resource utilization. This study explores the method of preparing regenerated magnets using waste magnets as raw materials based on existing processes. By utilizing existing Nd-Fe-B production equipment, various waste magnets are transformed into recycled powder. Next, nascent Nd-Fe-B powders with slightly higher rare earth content are selected as the repairing agent. The regenerated magnets are prepared by incorporating the nascent powder into the recycled powder. The focus lies in investigating the repairing effect of the nascent powder repairing agent on the microstructure of regenerated magnets and exploring the influence of sintering temperature and powder addition on the magnetic properties and microstructure of the regenerated magnets. The results showed that the nascent powder increased the proportion of grain boundary phases and effectively repaired the grain boundary structure of the regenerated magnets. In addition, the Pr element in the nascent powder replaces the Ce element in the recycled powder, which ultimately improves the magnetic properties of the regenerated magnet in a comprehensive manner. This study provides valuable insights and guidance for rare earth resource recycling and the preparation of regenerated magnets.
Evaluation of the Influence of Bolt Fastener Spacing on the Elastic Critical Load from the Lateral-Torsional Buckling Condition of Built-Up Bending Members
Authors: Piotrowski Rafał , Szychowski Andrzej
Published: July 2024

In an experimental study of two-branched beams bent transversely about the major stiffness axis, the elastic critical load from the lateral-torsional buckling condition was determined. The tests were conducted on simply supported two-branch beam models with a built-up section consisting of two cold-formed channel members (2C) bolted back-to-back. The bolts were located at the mid-height of the built-up cross-section. Five groups of members differing in longitudinal bolt spacing were examined. The models were gravitationally loaded (using ballast) at the centre of the beam span. This approach eliminated the undesirable effect of the lateral support of the beam, e.g., by the actuator head. The critical load, measured by the concentrated transverse force (Pz,cr), was determined using the modified Southwell method. It has been experimentally shown that, in built-up beams, there is an influence of bolt spacing on the elastic critical load from the lateral-torsional buckling condition. The lowest critical load capacity and the most non-linear behaviour of the built-up member were observed in beams bolted with only three bolts (at the supports and in the middle of the span). However, the experimental results obtained in this study show that increasing the number of bolts above a certain level (in the case of the tested models, it was seven bolts) does not result in a further increase in the critical load, which is a surprising result. The obtained values were 15 to 23% lower than the critical load determined numerically by the finite element method (LTBeamN) for an analogous element with a uniform I-section.
Misdiagnosis Based on Neoplastic Markers—Extremely High Alpha-Fetoprotein in Patients with Intrahepatic Cholangiocarcinoma with Literature Review of the Published Cases
Authors: Jakimów Krzysztof , Tekiela Natalia , Kozak Katarzyna , Peterek Robert , Kwaśniewska Anna , Pająk Jacek , Chudek Jerzy
Published: July 2024

Background: Alpha-fetoprotein (AFP) and carbohydrate antigen 19-9 (CA 19-9) are two tumor markers that are widely used in the differential diagnosis in patients with primary liver tumors. Very high levels of AFP are sporadically observed in patients with intrahepatic cholangiocarcinoma (ICC) and may cause an incorrect initial diagnosis of hepatocellular carcinoma (HCC). Methods: Two cases of tumors in cirrhotic livers were described, in which the initial diagnosis, based on very high AFP levels (Patient I: 10,464 ng/mL, Patient II: 2212 ng/mL, reference range: ≤8.04 ng/mL) was HCC. In addition, the PubMed database was searched for cases of ICC with elevated AFP. Discussion: In both individuals, liver cirrhosis was diagnosed, but there was no typical rapid “washout” in the contrast-enhanced computed tomography. Based on the histological assessment of samples obtained in the core biopsies, the initially assumed diagnosis of HCC was changed to ICC in both cases. Only nine cases of patients with ICC and high AFP levels were found in the PubMed database. The AFP levels ranged from slightly elevated to over 16,000 ng/mL. Conclusions: A very high AFP level does not necessarily correlate with the presence of HCC. Therefore, the diagnosis has to be verified histologically, when the radiological imaging is uncertain in patients with liver cirrhosis.
Unlocking the Potential: Angiotensin Receptor Neprilysin and Sodium Glucose Co-Transporter 2 Inhibitors for Right Ventricle Dysfunction in Heart Failure
Authors: Das Bibhuti B.
Published: July 2024

This review article examines the mechanism of action of Angiotensin Receptor–Neprilysin Inhibitors (ARNIs) and Sodium–Glucose Co-Transporter 2 Inhibitors (SGLT2is) in managing chronic right ventricular (RV) dysfunction. Despite advancements in heart failure (HF) treatment, RV dysfunction remains a significant contributor to morbidity and mortality. This article explores the The article explores the impact of ARNIs and SGLT2is on RV function based on clinical and preclinical evidence, and the potential benefits of combined therapy. It highlights the need for further research to optimize patient outcomes and suggests that RV function should be considered in future clinical trials as part of risk stratification for HF therapies. This review underscores the importance of the early initiation of ARNIs and SGLT2is as per guideline-directed medical therapy for eligible HFrEF and HFpEF patients to improve co-existing RV dysfunction.
The Cross-Sectional Area Ratio of Right-to-Left Portal Vein Predicts the Effect of Preoperative Right Portal Vein Embolization
Authors: Jo Yeongsoo , Lee Hae Won , Han Ho-Seong , Yoon Yoo-Seok , Cho Jai Young
Published: July 2024

Background and Objectives: Preoperative right portal vein embolization (RPVE) is often attempted before right hepatectomy for liver tumors to increase the future remnant liver volume (FRLV). Although many factors affecting FRLV have been discussed, few studies have focused on the ratio of the cross-sectional area of the right portal vein to that of the left portal vein (RPVA/LPVA). The aim of the present study was to evaluate the effect of RPVA/LPVA on predicting FRLV increase after RPVE. Materials and Methods: The data of 65 patients who had undergone RPVE to increase FRLV between 2004 and 2021 were investigated retrospectively. Using computed tomography scans, we measured the total liver volume (TLV), FRLV, the proportion of FRLV relative to TLV (FRLV%), the increase in FRLV% (ΔFRLV%), and RPVA/LPVA twice, immediately before and 2–3 weeks after RPVE; we analyzed the correlations among those variables, and determined prognostic factors for sufficient ΔFRLV%. Results: Fifty-four patients underwent hepatectomy. Based on the cut-off value of RPVA/LPVA, the patients were divided into low (RPVA/LPVA ≤ 1.20, N = 30) and high groups (RPVA/LPVA > 1.20, N = 35). The ΔFRLV% was significantly greater in the high group than in the low group (9.52% and 15.34%, respectively, p < 0.001). In a multivariable analysis, RPVA/LPVA (HR = 20.368, p < 0.001) was the most significant prognostic factor for sufficient ΔFRLV%. Conclusions: RPVE was more effective in patients with higher RPVA/LPVA, which is an easily accessible predictive factor for sufficient ΔFRLV%.
Accessible New Non-Quantum Dot Cs2PbI2Cl2-Based Photocatalysts for Efficient Hole-Driven Photocatalytic Applications
Authors: Huang Xing , Lv Kuanxin , Zhu Wenqiang , Li Zhenzhen , Zhao Hang
Published: July 2024

Efficient, low-cost photocatalysts with mild synthesis conditions and stable photocatalytic behavior have always been the focus in the field of photocatalysis. This study proves that non-quantum-dot Cs2PbI2Cl2-based materials, created by a simple method, can be successfully employed as new high-efficient photocatalysts. The results demonstrate that two-dimensional Cs2PbI2Cl2 perovskite can achieve over three times higher photocatalytic performance compared to three-dimensional CsPbBr3 perovskite. Moreover, the photocatalytic performance of Cs2PbI2Cl2 can be further improved by constructing a heterojunction structure, such as Cs2PbI2Cl2/CsPbBr3. Cs2PbI2Cl2 can connect well with CsPbBr3 through a simple method, resulting in tight bonding at the interface and efficient carrier transfer. Cs2PbI2Cl2/CsPbBr3 exhibits notable 5-fold and 10-fold improvements in photocatalytic performance and rate compared to CsPbBr3. Additionally, Cs2PbI2Cl2/CsPbBr3 demonstrates superb stable catalytic performance, with nearly no decrease in photocatalytic performance after 7 months (RH = 20% ± 10, T = 25 °C ± 5). This study also reveals that the photocatalytic process based on Cs2PbI2Cl2/CsPbBr3 can directly oxidize organic matter using holes, without relying on the generation of intermediate reactive oxygen species from water or oxygen (such as ·OH or ·O2−), showcasing further potential for achieving high photocatalytic efficiency and selectivity in anhydrous/anaerobic catalytic reactions and treating recalcitrant pollutants.
Self-Reconstructed Metal–Organic Framework-Based Hybrid Electrocatalysts for Efficient Oxygen Evolution
Authors: Cai Kunting , Chen Weibin , Wan Yinji , Chu Hsingkai , Hai Xiao , Zou Ruqiang
Published: July 2024

Refining synthesis strategies for metal–organic framework (MOF)-based catalysts to improve their performance and stability in an oxygen evolution reaction (OER) is a big challenge. In this study, a series of nanostructured electrocatalysts were synthesized through a solvothermal method by growing MOFs and metal–triazolates (METs) on nickel foam (NF) substrates (named MET-M/NF, M = Fe, Co, Cu), and these electrocatalysts could be used directly as OER self-supporting electrodes. Among these electrocatalysts, MET-Fe/NF exhibited the best OER performance, requiring only an overpotential of 122 mV at a current density of 10 mA cm−2 and showing remarkable stability over 15 h. The experimental results uncovered that MET-Fe/NF underwent an in situ structural reconstruction, resulting in the formation of numerous iron/nickel (oxy)hydroxides with high OER activity. Furthermore, in a two-electrode water-splitting setup, MET-Fe/NF only required 1.463 V to achieve a current density of 10 mA cm−2. Highlighting its potential for practical applications. This work provides insight into the design and development of efficient MOF-based OER catalysts.
Molecular and Biochemical Therapeutic Strategies for Duchenne Muscular Dystrophy
Authors: Krishna Lakshmi , Prashant Akila , Kumar Yogish H. , Paneyala Shasthara , Patil Siddaramappa J. , Ramachandra Shobha Chikkavaddaragudi , Vishwanath Prashant
Published: July 2024

Significant progress has been achieved in understanding Duchenne muscular dystrophy (DMD) mechanisms and developing treatments to slow disease progression. This review article thoroughly assesses primary and secondary DMD therapies, focusing on innovative modalities. The primary therapy addresses the genetic abnormality causing DMD, specifically the absence or reduced expression of dystrophin. Gene replacement therapies, such as exon skipping, readthrough, and gene editing technologies, show promise in restoring dystrophin expression. Adeno-associated viruses (AAVs), a recent advancement in viral vector-based gene therapies, have shown encouraging results in preclinical and clinical studies. Secondary therapies aim to maintain muscle function and improve quality of life by mitigating DMD symptoms and complications. Glucocorticoid drugs like prednisone and deflazacort have proven effective in slowing disease progression and delaying loss of ambulation. Supportive treatments targeting calcium dysregulation, histone deacetylase, and redox imbalance are also crucial for preserving overall health and function. Additionally, the review includes a detailed table of ongoing and approved clinical trials for DMD, exploring various therapeutic approaches such as gene therapies, exon skipping drugs, utrophin modulators, anti-inflammatory agents, and novel compounds. This highlights the dynamic research field and ongoing efforts to develop effective DMD treatments.