bluuebunny/crossref_metadata_2025 huggingface dataset:
YOLO-PEM: A Lightweight Detection Method for Young “Okubo” Peaches in Complex Orchard Environments
Authors: Jing Jianping , Zhang Shujuan , Sun Haixia , Ren Rui , Cui Tianyu
Published: August 2024

The intelligent detection of young peaches is the main technology of fruit-thinning robots, which is crucial for enhancing peach fruit quality and reducing labor costs. This study presents the lightweight YOLO-PEM model based on YOLOv8s to achieve high-precision and automatic detection of young “Okubo” peaches. Firstly, the C2f_P module was devised by partial convolution (PConv), replacing all C2f modules in YOLOv8s to achieve the model’s lightweight. Secondly, embedding the efficient multi-scale attention (EMA) module in the lightweight C2f_P_1 module of the backbone network enhanced the feature extraction capability and accuracy for young peaches. Finally, the MPDIoU loss function was utilized to replace the original CIoU loss function, which improved the detection accuracy of the bounding box while speeding up the convergence of the model. The experimental results demonstrate that the YOLO-PEM model achieved an average precision (AP) of 90.86%, F1 score of 86.70%, and model size of 16.1 MB, which was a 1.85% improvement in the AP, 0.85% improvement in the F1 score, and 5.3 MB reduction in the model size compared with YOLOv8s. The AP was 6.26%, 6.01%, 2.05%, 2.12%, and 1.87% higher compared with the other lightweight detection models YOLOv3-tiny, YOLOv4-tiny, YOLOv5s, YOLOv6s, and YOLOv7-tiny, respectively. Furthermore, the FPS of YOLO-PEM was 196.2 f·s-1, which can fulfill the demand for the real-time detection of young peaches. YOLO-PEM effectively detects young peaches in complex orchard environments and can offer a basis for the theoretical design of the vision system of the “Okubo” peach fruit-thinning robot and scientific management of orchards.
Tillage Practices Effect on Root Distribution and Variation of Soil CO2 Emission under Different Cropping Strategies
Authors: Buivydienė Agnė , Deveikytė Irena , Veršulienė Agnė , Feiza Virginijus
Published: August 2024

Conservation soil management strategies, particularly no-tillage and cropping strategies, have become an effective and widely adopted practice that has a direct influence on root parameters and mitigation of greenhouse gasses. However, the effect of different tillage and cropping strategies on root growth in field conditions is rarely studied. The study aimed to quantify and characterise the relationship between root network development and CO2 emission and how these parameters are affected by different cropping and tillage strategies. Five different crop rotations were tested, with or without the inclusion of catch crops (CC), by growing them in the soil where different tillage practices were applied. Selected cropping strategies differed among themselves in terms of the frequency of CC grown per rotation. The data revealed that in NT treatments, the CO2 emission (both autotrophic and heterotrophic simultaneously) was 25% higher than in CT. The cropping strategies were identified as an important factor influencing CO2 emissions. An increase in CO2 emission varied between 30 and 35% depending on the share of legume and CC inclusion. The frequency of CC grown per rotation has had an effect on the rate of CO2 emission. The cropping strategy when CC was grown every year showed the lowest amount of CO2 (by 26%), while in other cropping strategies, when CC was grown once or twice per rotation, significantly higher CO2 emissions were observed. Root growth and their development were significantly affected (p < 0.05) by soil depth and cropping strategies concerning root length and root volume changes. The inclusion of CC into the rotations led towards a decrease in root volume (by 21%). Root length (R2 = 0.45; p < 0.05) and root volume (R2 = 0.82; p < 0.05) had a significant impact on soil CO2 emissions. The results collected from 2021 to 2023 experiments indicated that cropping strategies and CC management areas are important tools not only for the improvement of root parameters but also for understanding how they affect CO2 emissions. The main message for stakeholders is that the cropping strategies diversification with the inclusion of CC every year in a winter oil-seed rape, spring wheat and pea crop rotation (R/W/P + CC) had demonstrated the possibilities to reduce CO2 emission and improve the root network parameters as compared to the monoculture strategy.
Exploration of Response Mechanisms in the Gills of Pacific Oyster (Crassostrea gigas) to Cadmium Exposure through Integrative Metabolomic and Transcriptomic Analyses
Authors: Dong Luyao , Sun Yanan , Chu Muyang , Xie Yuxin , Wang Pinyi , Li Bin , Li Zan , Xu Xiaohui , Feng Yanwei , Sun Guohua , Wang Zhongping , Cui Cuiju , Wang Weijun , Yang Jianmin
Published: August 2024

Marine mollusks, including oysters, are highly tolerant to high levels of cadmium (Cd), but the molecular mechanisms underlying their molecular response to acute Cd exposure remain unclear. In this study, the Pacific oyster Crassostrea gigas was used as a biological model, exposed to acute Cd stress for 96 h. Transcriptomic analyses of their gills were performed, and metabolomic analyses further validated these results. In our study, a total of 111 differentially expressed metabolites (DEMs) and 2108 differentially expressed genes (DEGs) were identified under acute Cd exposure. Further analyses revealed alterations in key genes and metabolic pathways associated with heavy metal stress response. Cd exposure triggered physiological and metabolic responses in oysters, including enhanced oxidative stress and disturbances in energy metabolism, and these changes revealed the biological response of oysters to acute Cd stress. Moreover, oysters could effectively enhance the tolerance and detoxification ability to acute Cd exposure through activating ABC transporters, enhancing glutathione metabolism and sulfur relay system in gill cells, and regulating energy metabolism. This study reveals the molecular mechanism of acute Cd stress in oysters and explores the molecular mechanism of high tolerance to Cd in oysters by using combined metabolomics and transcriptome analysis.
Comparison between Intramuscular and Intranasal Administration of Sedative Drugs Used for Piglet Castration
Authors: Breitenlechner Andreas , Bünger Moritz , Ruczizka Ursula Katharina , Dolezal Marlies , Auer Ulrike , Buzanich-Ladinig Andrea
Published: August 2024

The aim of this study was to test the intranasal administration of different anaesthetics in piglets less than seven days of age undergoing castration for their suitability for providing good-quality sedation and short induction and recovery time with minimal stress. Azaperone alone at a high (5 mg/kg), medium (3 mg/kg) and low dosage (2 mg/kg) and in two combinations with either alfaxalone or midazolam were applied intramuscularly (i.m.) or intranasally (i.n.) to 120 healthy piglets. Compared to intramuscular application, intranasal application showed longer induction times, shorter recovery times and higher scores for defence and vocalisation. In conclusion, the intranasal protocols did not meet the requirements in all groups and their use can therefore not be recommended. A rapid induction phase and good quality of sedation could not be guaranteed.
Quercetin Supplementation Improves Intestinal Digestive and Absorptive Functions and Microbiota in Rats Fed Protein-Oxidized Soybean Meal: Transcriptomics and Microbiomics Insights
Authors: Wang Zhiyong , Wang Peng , Zhou Yanmin , Zhuang Su
Published: August 2024

To clarify the nutritional mechanisms of quercetin mitigation in the digestive and absorptive functions in rats fed protein-oxidized soybean meal, 48 three-week-old male SD rats were randomly allocated into a 2 × 2 factorial design with two soybean meal types (fresh soybean meal or protein-oxidized soybean meal) and two quercetin levels (0 or 400 mg/kg) for a 28-day feeding trial. The protein-oxidized soybean meal treatment decreased (p < 0.05) the relative weights of the pancreas, stomach, and cecum, duodenal villus height, pancreatic and jejunal lipase activities, apparent ileal digestibility of amino acids, and apparent total tract digestibility of dry matter, crude protein, and ether extract. The supplementation of quercetin in the protein-oxidized soybean meal diet reversed (p < 0.05) the decreases in the duodenal length, ileal villus height, lipase activity, apparent ileal digestibility of amino acids, and apparent total tract digestibility of dry matter, crude protein, and ether extract. Transcriptomics revealed that the “alanine transport” and “lipid digestion and absorption” pathways were downregulated by the protein-oxidized soybean meal compared with fresh soybean meal, while the “basic amino acid transmembrane transporter activity” and “lipid digestion and absorption” pathways were upregulated by the quercetin supplementation. Microbiomics revealed that the protein-oxidized soybean meal increased the protein-degrading and inflammation-triggering bacteria in the cecum, while the relative abundances of beneficial bacteria were elevated by the quercetin supplementation.
Impact of Partial Oil Removal on Energy Content of Distillers Grains Plus Solubles for Finishing Cattle
Authors: Burhoop Jordan E. , Sperber Jessica L. , Bittner Curt J. , Hilscher F. Henry , MacDonald Jim C. , Erickson Galen E.
Published: August 2024

Two experiments evaluated the impact of the reduction in the percentage of corn oil remaining in distillers grains plus solubles (DGS) after the ethanol plant de-oiling process or by adding corn oil back to DGS following de-oiling on finishing cattle performance and nutrient digestion. Experiment 1 utilized 320 yearling steers (initial BW = 413 kg; SD = 25 kg) fed in 32 pens (10 steers/pen) and assigned to one of four treatments (n = 8 pens/treatment). The four treatments consisted of a blended DRC:HMC corn control diet (CON), de-oiled modified distillers grains plus solubles included at 40% of diet DM (DODGS), de-oiled modified distillers grains plus solubles included at 38% of diet DM plus 2% added corn oil (DODGS + Oil), and full-fat modified distillers grains plus solubles included at 40% of diet DM (FFDGS). The DODGS product contained 8.9% fat while the FFDGS product contained 11.6% fat. Dry matter intake (DMI) was impacted by treatment (p = 0.01) with steers fed DODGS having the greatest DMI and steers fed CON, DODGS + Oil, and FFDGS having lower DMI. Dietary treatment tended to impact ADG (p = 0.06) with steers fed DODGS and DODGS + Oil having greater gains than CON, with FFDGS being an intermediate. As a result of increased ADG, G:F differed between treatments (p < 0.01) with the greatest feed efficiency observed for steers fed DODGS + Oil and FFDGS. Including MDGS in the diet improved G:F by 6 to 11% compared to feeding DRC:HMC corn blend, with an improvement in G:F of 4.9 and 1.2% for DODGS + Oil and FFDGS, respectively, compared to DODGS. Hot carcass weight was impacted by dietary treatment (HCW; p = 0.05), with DODGS- and DODGS + Oil-fed steers having the heaviest HCW, CON steers having the lightest HCW, and FFDGS being an intermediate. Experiment 2 was a 5 × 4 unbalanced Latin rectangle digestion experiment with four diets, five ruminally cannulated steers, and five periods that utilized the same treatments as Exp. 1. Dietary fat measured 4.2, 6.0, 7.9, and 7.1% for CON, DODGS, DODGS + Oil, and FFDGS, respectively. Intakes of DM, OM, and energy as well as total tract fat digestibility and DE (Mcal/d) were not impacted by dietary treatment (p ≥ 0.46). When corn oil was added back to de-oiled MDGS, there was a negative impact on digestibility of OM (p < 0.01) and NDF (p = 0.07) compared with DODGS, FFDGS, and CON. Partially removing oil from modified distillers grains plus solubles did not significantly impact cattle performance, carcass traits, energy content, or digestibility when MDGS was included at approximately 40% of diet DM.
Review of Photovoltaic–Battery Energy Storage Systems for Grid-Forming Operation
Authors: Yin Kai , Xiao Yi , Shen Xiaomeng , Zhu Yinxiao , Yang Yongheng
Published: August 2024

Coordinated control technology attracts increasing attention to the photovoltaic–battery energy storage (PV-BES) systems for the grid-forming (GFM) operation. However, there is an absence of a unified perspective that reviews the coordinated GFM control for PV-BES systems based on different system configurations. This paper aims to fill the gap by providing a comprehensive review of coordinated GFM control strategies for PV-BES, considering various system configurations. Typical configurations of PV-BES systems are explored, followed by a detailed discussion of conventional GFM control methods used in the PV-BES systems. Furthermore, coordinated GFM controls are analyzed in PV-BES systems based on different configurations, providing the common DC bus configuration as a widely adopted configuration due to its more control degrees of freedom and ease of expansion. Moreover, the mode division and switching coordinated control based on the system power is the most widely used. Furthermore, challenges in the coordinated GFM controls for the PV-BES system in future applications are briefed and emphasized before the conclusion.
Differential Regulation of Wingless-Wnt/c-Jun N-Terminal Kinase Crosstalk via Oxidative Eustress in Primary and Metastatic Colorectal Cancer Cells
Authors: Aceto Gitana Maria , Pagotto Sara , Del Pizzo Francesco Domenico , Saoca Concetta , Selvaggi Federico , Visone Rosa , Cotellese Roberto , Aguennouz M’hammed , Lattanzio Rossano , Catalano Teresa
Published: August 2024

In the tumor microenvironment (TME), ROS production affects survival, progression, and therapy resistance in colorectal cancer (CRC). H2O2-mediated oxidative stress can modulate Wnt/β-catenin signaling and metabolic reprogramming of the TME. Currently, it is unclear how mild/moderate oxidative stress (eustress) modulates Wnt/β-catenin/APC and JNK signaling relationships in primary and metastatic CRC cells. In this study, we determined the effects of the H2O2 concentration inducing eustress on isogenic SW480 and SW620 cells, also in combination with JNK inhibition. We assessed cell viability, mitochondrial respiration, glycolysis, and Wnt/β-catenin/APC/JNK gene and protein expression. Primary CRC cells were more sensitive to H2O2 eustress combined with JNK inhibition, showing a reduction in viability compared to metastatic cells. JNK inhibition under eustress reduced both glycolytic and respiratory capacity in SW620 cells, indicating a greater capacity to adapt to TME. In primary CRC cells, H2O2 alone significantly increased APC, LEF1, LRP6, cMYC and IL8 gene expression, whereas in metastatic CRC cells, this effect occurred after JNK inhibition. In metastatic but not in primary tumor cells, eustress and inhibition of JNK reduced APC, β-catenin, and pJNK protein. The results showed differential cross-regulation of Wnt/JNK in primary and metastatic tumor cells under environmental eustress conditions. Further studies would be useful to validate these findings and explore their therapeutic potential.
Differences in the Interleukin Profiles in Inattentive ADHD Prepubertal Children Are Probably Related to Conduct Disorder Comorbidity
Authors: González-Villén Raquel , Fernández-López María Luisa , Checa-Ros Ana , Tortosa-Pinto Pilar , Aguado-Rivas Raquel , Garre-Morata Laura , Acuña-Castroviejo Darío , Molina-Carballo Antonio
Published: August 2024

Inflammatory cytokines are involved in attention deficit hyperactivity disorder (ADHD), a highly prevalent neurodevelopmental disorder. To quantify the baseline levels of pro- and anti-inflammatory cytokines and their changes after methylphenidate (MPH), a total of 31 prepubertal children with ADHD were recruited and subclassified into only two ADHD presentations—ADHD attention deficit (n = 13) or ADHD combined (n = 18). The children were also screened for oppositional defiant conduct disorder (ODCD) and anxiety disorder. Blood samples were drawn at 09:00 and after 4.63 ± 1.87 months of treatment. Four pro-inflammatory cytokines (interleukin-1beta (IL-1β), IL-5, IL-6, tumor necrosis factor-alpha (TNF-α)) and three anti-inflammatory cytokines (IL-4, IL-10, IL-13) were measured using a Luminex® assay. For statistics, a factorial analysis was performed in Stata 15.1. Overall, there were no statistically significant differences in the interleukin (IL) values induced by treatment. When grouped by presentation, the differences were present almost exclusively in ADHD-AD, usually with a profile opposite to that observed in ADHD-C, and with interactions between comorbid factors, with IL-1β (p = 0.01) and IL-13 (p = 0.006) being the ones reaching the greatest statistical significance. These differences are probably related to the ODCD factor, and they disappear after treatment. In conclusion, the changes observed in cytokine levels in prepubertal children only in the ADHD-AD presentation are probably related to comorbidities (specifically ODCD) and are mitigated after treatment.
Detecting Nanotopography Induced Changes in Cell Migration Directions Using Oxygen Sensors
Authors: Wang Muting , Pang Stella W.
Published: August 2024

This study investigates the oxygen (O2) consumption of single cells during changes in their migration direction. This is the first integration of nanotopographies with an O2 biosensor in a platform, allowing the real-time monitoring of O2 consumption in cells and the ability to distinguish cells migrating in the same direction from those migrating in the opposite direction. Advanced nanofabrication technologies were used to pattern nanoholes or nanopillars on grating ridges, and their effects were evaluated using fluorescence microscopy, cell migration assays, and O2 consumption analysis. The results revealed that cells on the nanopillars over grating ridges exhibited an enhanced migration motility and more frequent directional changes. Additionally, these cells showed an increased number of protrusions and filopodia with denser F-actin areas and an increased number of dotted F-actin structures around the nanopillars. Dynamic metabolic responses were also evident, as indicated by the fluorescence intensity peaks of platinum octaethylporphyrin ketone dye, reflecting an increased O2 consumption and higher mitochondria activities, due to the higher energy required in response to directional changes. The study emphasizes the complex interplay between O2 consumption and cell migration directional changes, providing insights into biomaterial science and regenerative medicine. It suggests innovative designs for biomaterials that guide cell migration and metabolism, advocating nanoengineered platforms to harness the intricate relationships between cells and their microenvironments for therapeutic applications.
Reinforcement of Insufficient Transverse Connectivity in Prestressed Concrete Box Girder Bridges Using Concrete-Filled Steel Tube Trusses and Diaphragms: A Comparative Study
Authors: Li Peng , Yang Caiqian , Xu Fu , Li Junshi , Jin Dongzhao
Published: August 2024

To address the issue of insufficient transverse connectivity in prestressed concrete box girder (PCB) bridges, this study investigates two transverse strengthening methods—installing diaphragms and utilizing concrete-filled steel tube trusses (CFSTTs). A finite element model was developed for a typical 30 m PCB bridge and was validated by on-site load test results for reliability. Based on the deflection and load distribution of PCB bridges before and after reinforcement, as well as the maximum stress and strain of the diaphragms and the CFSTTs, comparative analyses were conducted on diaphragms of different thicknesses and materials, as well as on CFSTTs of various strength grades. The results show that the addition of a transverse partition and CFSTTs can effectively improve the load distribution of the PCB bridge and reduce the maximum deflection of the girder, especially when using the CFSTT reinforcement method. The unique structural design improves the reinforcement effect of the material in the post-elastic stage. When using CFSTTs, increasing the steel tube wall strength significantly reduces the maximum deflection of the main girder. For example, using steel tubes with yield strengths of 235 MPa and 420 MPa filled with concrete of 50 MPa compressive strength reduced the maximum deflections by 15.32% and 24.55%, respectively, and improved the load distribution coefficients by up to 7.31% and 11.57%. Additionally, steel diaphragms demonstrated better reinforcement effects compared with concrete diaphragms. The load transverse distribution coefficients for the CFSTT-reinforced PCB bridge were calculated using the hinge plate (beam) and the rigid plate (beam) methods, showing minimal differences between the two approaches. The findings of this study provide valuable insights into the design of diaphragm and CFSTT reinforcement in PCB bridges, aiding in the selection of optimal reinforcement strategies.
Investigating User Feedback for Learning Space Design in Primary Schools of Shandong Province, China
Authors: Sun Rongrong , Firzan Muhammad
Published: August 2024

A well-designed learning environment is crucial for enhancing both the physical and mental health of students, which in turn improves their learning outcomes. However, many classrooms in China, particularly in rural areas, were constructed and designed several decades ago, so it is essential to redesign these learning spaces to align with the requirements of 21st Century education. This study aims to develop the stimulation, individuality, and naturalness (SIN) theoretical framework for identifying the learning environment of current classroom by examining the full range of sensory effects experienced by individuals. This study conducted qualitative interviews with 72 students and 18 class teachers to explore major issues with their existing learning spaces in four primary schools in Shandong Province of China. The results show that high temperatures and poor air quality are frequently raised by respondents, which directly impact students’ learning experience. This finding confirms naturalness likely underpins human comfort. Teachers and students felt that the classroom should be improved from the aspects of crowded space, imperceptible decoration, congestion and monotonous layouts. The study emphasised the important factors that designers and policymakers should consider to promote a comfortable, efficient, and healthy learning environment.
Bearing Capacity of Hybrid (Steel and GFRP) Reinforced Columns under Eccentric Loading: Theory and Experiment
Authors: Pang Lei , Han Zebin , Xiao Jie , Liu Zexuan , Qu Wenjun , Dong Sansheng
Published: August 2024

In order to reveal the mechanical behavior of short concrete columns reinforced with hybrid steel and glass FRP bars, 10 specimens were designed for eccentric compression tests. The effect of eccentricity and load–displacement/strain of the specimens was studied. Test results indicate that the damage process and failure mode of these hybrid RC columns was similar to those in the conventional steel-reinforced concrete columns. The mode of failure for all specimens is characterized as large eccentricity compression failure, and the ultimate bearing capacity of the columns decreases with the increase in eccentricity. However, the impact of the varying axial stiffness ratio between GFRP and steel bars on the bearing capacity can be considered negligible. In addition, based on theoretical analysis, two boundary states for distinguishing failure mode and the formulae for calculating ultimate bearing capacity in different failure modes of eccentrically loaded hybrid RC columns are proposed. The computed results agree well with test results.
Aerodynamic Behavior of Hump Slab Track in Desert Railways: A Case Study in Shuregaz, Iran
Authors: Fathali Masoud , Nasrabad Mohammad Mohsen Kabiri , Nejad Fereidoon Moghadas , Chalabii Jafar , Rad Majid Movahedi
Published: August 2024

The development of rail transport necessitates expanding environmentally friendly infrastructure. However, specific challenges arise in desert and sandy regions. One innovative solution to manage the effects of windblown sand on desert railways is the use of hump slab track superstructure. This paper develops a solid–fluid aerodynamic model based on ANSYS Fluent 2021 R2 software to simulate the hump slab track during a sandstorm. The model is validated through wind tunnel testing. A case study of a railway sandstorm in the Shuregaz region of Iran is presented, evaluating various sandstorm parameters and hump heights to determine their impact on sand concentration and particle velocity within the sand transit channels. The results indicate that increasing the sand particle diameter (from 150 to 250 µm) leads to higher sand concentration (up to 40%) and lower sand movement velocity (up to 28%). These results have been observed with a higher incremental approach concerning the sand flow rate. Conversely, increasing sandstorm velocity (from 10 to 30 m/s) decreases sand concentration and increases sand movement velocity up to 80% and 150%, respectively. Additionally, a 25 cm hump height significantly enhances sand passage by creating larger channels.
Global Stability Behavior of Pre-Cast Cable-Stiffened Steel Columns
Authors: Zhao Ying , Hu Junxiu , Song Shushuang , Zhang Tianhao , Li Pengcheng , Xiong Gang
Published: August 2024

Cable-stiffened steel columns (CSSC) have a high load-carrying capacity and strong stability compared to ordinary steel columns. In practical engineering, the connection between the crossarm and main column of a CSSC is usually welded. However, the welding-residual stress adversely affects the steel column. In this study, pre-cast CSSCs, with a pinned connection between the crossarm and main column, are presented. The new type of pre-cast CSSCs avoid the welding-residual and are easy to disassemble. A model test and numerical analysis of its global stability behavior under eccentric compression is conducted. Based on the analysis, the buckling modes of these columns are defined and a method for determining the governing imperfection in a nonlinear buckling analysis is proposed. The effects of slenderness ratio, cross-arm length, cable diameter, and other parameters on the load-carrying capacities of the columns are investigated using the proposed method. The results of this study can be used as a reference for the engineering designs and specifications of pre-cast CSSCs.
Experimental and Theoretical Study on the Interface Mechanical Performance of Aluminum–Timber Composite Connections
Authors: Zheng Tengteng , Zhao Caiqi , Zhang Yikang
Published: August 2024

The interface mechanical performance between aluminum alloy and timber is the key to ensure that the two work together. In this study, 11 group connection performance tests were carried out to investigate the influence of connection type (shear bolt connection, epoxy resin adhesive connection, and mixed connection), number and spacing of bolts, thickness, and length (the area of incidence of the adhesive in the structure) of epoxy resin adhesive on the interface shear-resistant capacity of aluminum–timber composite connections. The shear performance of the three kinds of connections were studied via finite element analysis, and the calculation formula for interface shear-resistant capacity of the aluminum–timber composite connection was proposed based on the bond-slip mechanism of adhesive. The analysis results indicate that the mixed connection can avoid the brittle failure characteristics of the shear bolt connection and the epoxy resin adhesive connection, and the shear-resistant capacity is increased by 45.6% and 14.7%, respectively. The results of the calculation formula for interface shear-resistant capacity are in good agreement with the experimental results, indicating that it is suitable for the aluminum–timber composite connection.
Evaluation and Division Methods of Coupled Spatial Units between Parks and Living Circles: A Case Study of Four National-Level New Areas
Authors: Wang Chenpei , Yang Yuanxiang , Li Gantian , Yan Shuiyu
Published: August 2024

This study, based on the perspective of supply and demand coupling between parks and living circles, divides spatial units by introducing the Thiessen polygon method with urban parks as the core. Simultaneously, the coefficient of variation (CV) was used to evaluate the distribution characteristics of these spatial units. The suitability of the spatial units was comprehensively evaluated through the three indicators of park service support, functional concentration of living circle, and management and development level. The Node–Place (NP) model was used to quantify the coupling relationship between the park and the living circle, and the characteristics of the coupling units were deeply analyzed from the aspects of supply and demand services, coupling configuration relationship, development management, etc. The results are empirically applied to four national-level new areas, thereby providing a new evaluation method and perspective for urban planning and management.
Conventional Spinal Chordomas: Investigation of SMARCB1/INI1 Protein Expression, Genetic Alterations in SMARCB1 Gene, and Clinicopathological Features in 89 Patients
Authors: Maioli Margherita , Cocchi Stefania , Gambarotti Marco , Benini Stefania , Magagnoli Giovanna , Gamberi Gabriella , Griffoni Cristiana , Gasbarrini Alessandro , Ghermandi Riccardo , Noli Luigi Emanuele , Alcherigi Chiara , Ferrari Cristina , Bianchi Giuseppe , Asioli Sofia , Pignotti Elettra , Righi Alberto
Published: August 2024

The partial loss of SMARCB1/INI1 expression has recently been reported in skull base conventional chordomas, with possible therapeutic implications. We retrospectively analyzed 89 patients with conventional spinal chordomas to investigate the differences in the immunohistochemical expression of SMARCB1/INI1 and the underlying genetic alterations in the SMARCB1 gene. Moreover, we assessed the correlation of clinicopathological features (age, gender, tumor size, tumor location, surgical margins, Ki67 labelling index, SMARCB1/INI1 pattern, previous surgery, previous treatment, type of surgery, and the Charlson Comorbidity Index) with patient survival. Our cohort included 51 males and 38 females, with a median age at diagnosis of 61 years. The median tumor size at presentation was 5.9 cm. The 5-year overall survival (OS) and 5-year disease-free survival (DFS) rates were 90.8% and 54.9%, respectively. Partial SMARCB1/INI1 loss was identified in 37 (41.6%) patients with conventional spinal chordomas (27 mosaic and 10 clonal). The most frequent genetic alteration detected was the monoallelic deletion of a portion of the long arm of chromosome 22, which includes the SMARCB1 gene. Partial loss of SMARCB1/INI1 was correlated with cervical–thoracic–lumbar tumor location (p = 0.033) and inadequate surgical margins (p = 0.007), possibly due to the high degree of tumor invasiveness in this site. Among all the considered clinicopathological features related to patient survival, only tumor location in the sacrococcygeal region and adequate surgical margins positively impacted DFS. In conclusion, partial SMARCB1/INI1 loss, mostly due to 22q deletion, was detected in a significant number of patients with conventional spinal chordomas and was correlated with mobile spine location and inadequate surgical margins.
Efficacy and Safety of Sorafenib or Lenvatinib for Advanced Hepatocellular Carcinoma after Failure of First-Line Atezolizumab Plus Bevacizumab: A Systematic Review and Meta-Analysis
Authors: Peng Tzu-Rong , Weng Yi-Fang , Wu Ta-Wei , Wu Chao-Chuan , Chou Yi-Chun , Hsu Ching-Sheng
Published: August 2024

Background: Although atezolizumab plus bevacizumab (hereinafter, atezolizumab–bevacizumab) is the standard first-line treatment for patients with advanced HCC, the optimal second-line regimen remains unknown. This study evaluated the efficacy and safety of sorafenib and lenvatinib in patients with advanced HCC that progressed under atezolizumab–bevacizumab treatment. Methods: Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, we searched PubMed, Embase, and the Cochrane Library for articles published before November 2023. Random-effects meta-analysis was performed to determine the pooled objective response rate (ORR), disease control rate (DCR), progression-free survival (PFS), and overall survival (OS), comparing patients who received sorafenib versus lenvatinib. Results: Seven studies involving 387 patients were included. The pooled ORR, DCR, OS, and PFS for sorafenib and lenvatinib together were 26% (95% CI: 14–43%), 63% (95% CI: 47–77%), 11.45 months (95% CI: 7.12–15.77, I2 = 92%, p < 0.01), and 3.78 months (95% CI: 2.34–5.23, I2 = 67%, p = 0.02), respectively. Although lenvatinib users had a longer median OS (12.42 vs. 10.75 months) and PFS (5.15 vs. 2.58 months) than sorafenib users, the pooled ORR, DCR, median OS, and PFS for these medications were comparable. Additionally, the distributions of all-grade and grade ≥ 3 adverse events for sorafenib and lenvatinib were comparable to those for these two medications when used as first-line therapies. Conclusions: Sorafenib or lenvatinib can provide effective treatment with manageable toxicity in patients with advanced HCC after disease progression under atezolizumab–bevacizumab.
Role of MicroRNA-204 in Regulating the Hallmarks of Breast Cancer: An Update
Authors: Bermúdez Mercedes , Martínez-Barajas Marcela Guadalupe , Bueno-Urquiza Lesly Jazmín , López-Gutiérrez Jorge Armando , Villegas-Mercado Carlos Esteban , López-Camarillo César
Published: August 2024

microRNA-204-5p (miR-204) is a small noncoding RNA with diverse regulatory roles in breast cancer (BC) development and progression. miR-204 is implicated in the instauration of fundamental traits acquired during the multistep development of BC, known as the hallmarks of cancer. It may act as a potent tumor suppressor by inhibiting key cellular processes like angiogenesis, vasculogenic mimicry, invasion, migration, and metastasis. It achieves this by targeting multiple master genes involved in these processes, including HIF-1α, β-catenin, VEGFA, TGFBR2, FAK, FOXA1, among others. Additionally, miR-204 modulates signaling pathways like PI3K/AKT and interacts with HOTAIR and DSCAM-AS1 lncRNAs, further influencing tumor progression. Beyond its direct effects on tumor cells, miR-204 shapes the tumor microenvironment by regulating immune cell infiltration, suppressing pro-tumorigenic cytokine production, and potentially influencing immunotherapy response. Moreover, miR-204 plays a crucial role in metabolic reprogramming by directly suppressing metabolic genes within tumor cells, indirectly affecting metabolism through exosome signaling, and remodeling metabolic flux within the tumor microenvironment. This review aims to present an update on the current knowledge regarding the role of miR-204 in the hallmarks of BC. In conclusion, miR-204 is a potential therapeutic target and prognostic marker in BC, emphasizing the need for further research to fully elucidate its complex roles in orchestrating aggressive BC behavior.
Effect of Microcapsules of Chitosan-Coated Toddalia asiatica (L.) Lam Extracts on the Surface Coating Properties of Poplar Wood
Authors: Zhu Ye , Wang Ying , Yan Xiaoxing
Published: August 2024

Using chitosan as the shell material and Toddalia asiatica (L.) Lam extract as the core material, microcapsules of chitosan-coated Toddalia asiatica (L.) Lam extracts were prepared. The microcapsules were added to waterborne topcoats to investigate the effects of different content and MToddalia asiatica(L.) Lam extracts:Mchitosan (MT:MC) on the performance of waterborne coatings on poplar surfaces. Under different MT:MC of microcapsules, the content of microcapsules in the coating was negatively correlated with the glossiness, reflectivity, and adhesion of the coating. The addition of microcapsules reduced the liquid resistance of the coating to citric acid and improved the ethanol and cleaning agent resistance of the coating. The hardness, impact resistance, and roughness of the coatings increased gradually with the increase in microcapsule content. The content of microcapsules was positively correlated with the Escherichia coli and Staphylococcus aureus antibacterial performance of coatings, and the coatings had a slightly higher antibacterial rate against Staphylococcus aureus than Escherichia coli overall. The poplar surface coating with 5.0% microcapsules and MT:MC of 4.0:1 was excellent: the gloss was 5.30 GU, the light loss rate was 62.22%, the color difference ΔE was 22.93, the hardness was HB, the impact resistance was grade 3, the adhesion was grade 2, the roughness was 2.022 µm, the resistance to ethanol and cleaning agent was grade 2, and the resistances to Escherichia coli and Staphylococcus aureus were 74.21% and 82.01%, respectively. The results of the study provide a technical reference for the application of antibacterial waterborne coatings on wood surfaces.
Endurance to Multiple Factors of Water-Based Electrically Conductive Paints with Metallic Microparticles
Authors: Caramitu Alina Ruxandra , Ciobanu Romeo Cristian , Aradoaei Mihaela , Lungu Magdalena Valentina , Nicula Nicoleta Oana , Lungulescu Eduard Marius
Published: August 2024

The paper describes the innovative adaptation of some specific environmental tests from general organic coatings towards newly developed water-based composite paints with metallic particles (Al and Fe), with a high content of metal (10% and respectively 20%) for electromagnetic shielding applications. Electrical conductivity is the most affected dielectric parameter under both by UV radiation and thermal exposure. The paints with 20% metallic powder are more sensitive to environmental factors, and the influence of metal type could also be emphasized in relation to the dielectric feature evolution vs. exposure time. The action of mold significantly decreases the dielectric features of paints, but the weathering aging effect is much more enhanced if the samples are cumulatively submitted to thermal aging and respectively UV exposure, along with the action of mold. The potential application of the study is related mainly to the development of new autonomous electric cars, which need special conditions of electromagnetic shielding, under the circumstances that the conductive paint layers are normally very sensitive to environmental factors, affecting the equipment performance and security.
Effects of ALD Deposition Cycles of Al2O3 on the Morphology and Performance of FTO-Based Dye-Sensitized Solar Cells
Authors: Addae Elizabeth Adzo , Sitek Wojciech , Szindler Marek , Fijalkowski Mateusz , Matus Krzysztof
Published: August 2024

In dye-sensitized solar cells (DSSCs), materials classified as Transparent Conducting Oxides (TCOs) have the capacity to conduct electricity and transmit light at the same time. Their exceptional blend of optical transparency and electrical conductivity makes them popular choices for transparent electrodes in DSSCs. Fluorine Tin Oxide (FTO) was utilized in this experiment. The optical and electrical characteristics of TCOs may be negatively impacted by their frequent exposure to hostile environments and potential for deterioration. TCOs are coated with passivating layers to increase their performance, stability, and defense against environmental elements including oxygen, moisture, and chemical pollutants. Because of its superior dielectric qualities, strong chemical stability, and suitability with TCO materials, aluminum oxide (Al2O3) was utilized as a passivating layer for the FTO. In this research work, Al2O3 was deposited via atomic layer deposition (ALD) to form thin mesoporous layers as a passivator in the photoanode (working electrode). The work focuses on finding an appropriate thickness of Al2O3 for optimum performance of the dye-sensitized solar cells. The solar simulation and sheet resistance analysis clearly showed 200 cycles of Al2O3 to exhibit an efficiency of 4.31%, which was the most efficient performance. The surface morphology and topography of all samples were discussed and analyzed.
Exploring New Traveling Wave Solutions to the Nonlinear Integro-Partial Differential Equations with Stability and Modulation Instability in Industrial Engineering
Authors: Borhan J. R. M. , Abouelfarag I. , El-Rashidy K. , Miah M. Mamun , Iqbal M. Ashik , Kanan Mohammad
Published: August 2024

In this research article, we demonstrate the generalized expansion method to investigate nonlinear integro-partial differential equations via an efficient mathematical method for generating abundant exact solutions for two types of applicable nonlinear models. Moreover, stability analysis and modulation instability are also studied for two types of nonlinear models in this present investigation. These analyses have several applications including analyzing control systems, engineering, biomedical engineering, neural networks, optical fiber communications, signal processing, nonlinear imaging techniques, oceanography, and astrophysical phenomena. To study nonlinear PDEs analytically, exact traveling wave solutions are in high demand. In this paper, the (1 + 1)-dimensional integro-differential Ito equation (IDIE), relevant in various branches of physics, statistical mechanics, condensed matter physics, quantum field theory, the dynamics of complex systems, etc., and also the (2 + 1)-dimensional integro-differential Sawda–Kotera equation (IDSKE), providing insights into the several physical fields, especially quantum gravity field theory, conformal field theory, neural networks, signal processing, control systems, etc., are investigated to obtain a variety of wave solutions in modern physics by using the mentioned method. Since abundant exact wave solutions give us vast information about the physical phenomena of the mentioned models, our analysis aims to determine various types of traveling wave solutions via a different integrable ordinary differential equation. Furthermore, the characteristics of the obtained new exact solutions have been illustrated by some figures. The method used here is candid, convenient, proficient, and overwhelming compared to other existing computational techniques in solving other current world physical problems. This article provides an exemplary practice of finding new types of analytical equations.
Biocompatibility and Post-Marketing Surveillance Study of Emollient Plus Medical Device Cream Containing Oligofructans from Ophiopogon japonicus and Acetyl Heptapeptide-4 in Atopic Dermatitis Skin Care
Authors: Rachalewski Michał , Pasikowska-Piwko Monika , Dębowska Renata , Marczak Iwona , Lendzion Karolina , Godziątkowski Hubert , Czarnomysy Robert , Rogiewicz Katarzyna , Eris Irena
Published: August 2024

Emollients plus are defined as topical formulations containing active ingredients with no pharmacological effect. They are designed to target multiple mechanisms in AD pathophysiology. The objective of the present study was to assess the efficacy of emollient plus medical device cream by performing a post-marketing surveillance study. It was carried out in cooperation with 88 members of the Polish Association for Atopic Diseases who were diagnosed with AD and voluntarily fulfilled the questionnaire after 14 days of product use. Additionally, the medical device underwent in vitro/ex vivo testing. Cytotoxicity was assessed by in vitro studies: direct MTT assay and indirect Agarose Overlay Assay. An ex vivo EpiDerm™ culture (EPI-200) was used to investigate the irritation potential, and culture medium was collected after 18 h of contact with the skin model to perform a flow cytometric for the analysis of inflammatory cytokines. A dermatological assessment with the local SCORAD was employed to confirm the efficacy of the cream. It was found that 86% of patients with AD observed an improvement in their skin condition during the two-week testing period. In vitro/ex vivo assays confirmed that the product is safe, non-irritant, and does not stimulate the production of proinflammatory cytokines. According to the local SCORAD, the symptoms of AD were alleviated. Moreover, preliminary studies indicated its efficacy in eliminating S. aureus on patients’ skin.
Synergistic Threats to Wild Fauna in Ecuador: Using a Novel Data Source to Estimate the Impacts of Trafficking and Human–Wildlife Conflict
Authors: Villalba-Briones Ricardo , Mendoza Patricia , Garces Daniel , Belen Molineros Eliana , Monros Juan S. , Shanee Sam
Published: August 2024

Wildlife trafficking and human–wildlife conflict are major causes of species decline. The illegal nature of wildlife trafficking makes it hard, and sometimes dangerous, to study. ‘Mansión Mascota’ is a veterinary clinic in Guayaquil, Ecuador, which, through agreement with the Ecuadorian Ministry of the Environment, receives confiscated wildlife for treatment. Mansión Mascota also receives injured and abandoned wildlife brought in by the authorities and the public. Between January 2018 and September 2022, the clinic received 3212 wild animals from ≥171 taxa, including mammals, birds, reptiles, and amphibians. Through the classification of records based on reported use, native distribution, and evidence of capture/pet keeping, we were able to classify 1127 animals of 68 species as subject to wildlife trafficking. Turtles were the most abundant group (69%). The majority of turtles (91%) and primates (80%) were of Amazonian origin, whereas 90% of psittacines and 97% of carnivores had potential coastal origins. Threatened and conservation-dependent species were common in the traffic. Furthermore, ≥955 animals of 106 species were brought to the clinic due to anthropogenic wildlife conflict. Trafficking, combined with the high number of injured animals, highlights the synergistic threats facing wildlife in Ecuador.