bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and Validation of the Adolescent Sexting Scale (A-SextS) with a Spanish Sample
Authors: Molla Esparza Cristian , Nájera Pablo , López-González Emelina , Losilla Josep-Maria
Published: November 2020

“Sexting” is generally defined as the exchange of sexual media content via the internet. However, research on this topic has underscored the need to seek greater consensus when considering different conceptual elements that make up this definition. The aim of this study was to develop and validate an instrument for measuring sexting among adolescents, in order to cover a gap identified in the previous literature. The Adolescent Sexting Scale (A-SextS for short) was developed for validation on a sample of 579 Spanish secondary school pupils between the ages of 11 and 18. Evidence for face, content, concurrent, and criterion validity were assessed. A comprehensive set of 64 items, covering six defining characteristics of sexting (e.g., actions, recipient, media format, degree of sexual explicitness), was constructed after conducting an extensive literature review, two discussion groups, and a pilot study. Sexting prevalence rates measured by A-SextS were mostly concurrent with those found in previous studies. A-SextS subscales produced statistically significant positive associations with pornography consumption and physical sexual intercourse. The study shows that A-SextS can be an integrating instrument that facilitates a rigorous and comprehensive assessment of adolescent sexting experiences, as well as the formulation of an operationalized definition of the practice of sexting.
A Continuous, Semi-Automated Workflow: From 3D City Models with Geometric Optimization and CFD Simulations to Visualization of Wind in an Urban Environment
Authors: Deininger Martina E. , von der Grün Maximilian , Piepereit Raul , Schneider Sven , Santhanavanich Thunyathep , Coors Volker , Voß Ursula
Published: November 2020

The concept and implementation of Smart Cities is an important approach to improve decision making as well as quality of life of the growing urban population. An essential part of this is the presentation of data from different sources within a digital city model. Wind flow at building scale has a strong impact on many health and energy issues in a city. For the analysis of urban wind, Computational Fluid Dynamics (CFD) has become an established tool, but requires specialist knowledge to prepare the geometric input during a time-consuming process. Results are available only as predefined selections of pictures or videos. In this article, a continuous, semi-automated workflow is presented, which ❶ speeds-up the preparation of CFD simulation models using a largely automated geometry optimization; and ❷ enables web-based interactive exploration of urban wind simulations to a large and diverse audience, including experts and layman. Results are evaluated based on a case study using a part of a district in Stuttgart in terms of: ➀ time saving of the CFD model preparation workflow (85% faster than the manual method), ➁ response time measurements of different data formats within the Smart City platform (3D Tiles loaded 30% faster than geoJSON using the same data representations) and ➂ protocols (3DPS provided much higher flexibility than static and 3D container API), as well as ➃ subjective user experience analysis of various visualization schemes of urban wind. Time saving for the model optimization may, however, vary depending on the data quality and the extent of the study area.
Molecular Mechanisms of Laparoscopic Ovarian Drilling and Its Therapeutic Effects in Polycystic Ovary Syndrome
Authors: Seow Kok-Min , Chang Yi-Wen , Chen Kuo-Hu , Juan Chi-Chang , Huang Chen-Yu , Lin Li-Te , Tsui Kuan-Hao , Chen Yi-Jen , Lee Wen-Ling , Wang Peng-Hui
Published: November 2020

Polycystic ovary syndrome (PCOS) is a common endocrinopathy, characterized by chronic anovulation, hyperandrogenism, and multiple small subcapsular cystic follicles in the ovary during ultrasonography, and affects 5–10% of women of reproductive age. PCOS is frequently associated with insulin resistance (IR) accompanied by compensatory hyperinsulinemia and, therefore, presents an increased risk of type 2 diabetes mellitus (DM). The pathophysiology of PCOS is unclear, and many hypotheses have been proposed. Among these hypotheses, IR and hyperandrogenism may be the two key factors. The first line of treatment in PCOS includes lifestyle changes and body weight reduction. Achieving a 5–15% body weight reduction may improve IR and PCOS-associated hormonal abnormalities. For women who desire pregnancy, clomiphene citrate (CC) is the front-line treatment for ovulation induction. Twenty five percent of women may fail to ovulate spontaneously after three cycles of CC treatment, which is called CC-resistant PCOS. For CC-resistant PCOS women, there are many strategies to improve ovulation rate, including medical treatment and surgical approaches. Among the various surgical approaches, one particular surgical method, called laparoscopic ovarian drilling (LOD), has been proposed as an alternative treatment. LOD results in an overall spontaneous ovulation rate of 30–90% and final pregnancy rates of 13–88%. These benefits are more significant for women with CC-resistant PCOS. Although the intra- and post-operative complications and sequelae are always important, we believe that a better understanding of the pathophysiological changes and/or molecular mechanisms after LOD may provide a rationale for this procedure. LOD, mediated mainly by thermal effects, produces a series of morphological and biochemical changes. These changes include the formation of artificial holes in the very thick cortical wall, loosening of the dense and hard cortical wall, destruction of ovarian follicles with a subsequently decreased amount of theca and/or granulosa cells, destruction of ovarian stromal tissue with the subsequent development of transient but purulent and acute inflammatory reactions to initiate the immune response, and the continuing leakage or drainage of “toxic” follicular fluid in these immature and growth-ceased pre-antral follicles. All these factors contribute to decreasing local and systemic androgen levels, the following apoptosis process with these pre-antral follicles to atresia; the re-starting of normal follicular recruitment, development, and maturation, and finally, the normalization of the “hypothalamus–pituitary–ovary” axis and subsequent spontaneous ovulation. The detailed local and systematic changes in PCOS women after LOD are comprehensively reviewed in the current article.
ER–Mitochondria Contact Sites Reporters: Strengths and Weaknesses of the Available Approaches
Authors: Giamogante Flavia , Barazzuol Lucia , Brini Marisa , Calì Tito
Published: November 2020

Organelle intercommunication represents a wide area of interest. Over the last few decades, increasing evidence has highlighted the importance of organelle contact sites in many biological processes including Ca2+ signaling, lipid biosynthesis, apoptosis, and autophagy but also their involvement in pathological conditions. ER–mitochondria tethering is one of the most investigated inter-organelle communications and it is differently modulated in response to several cellular conditions including, but not limited to, starvation, Endoplasmic Reticulum (ER) stress, and mitochondrial shape modifications. Despite many studies aiming to understand their functions and how they are perturbed under different conditions, approaches to assess organelle proximity are still limited. Indeed, better visualization and characterization of contact sites remain a fascinating challenge. The aim of this review is to summarize strengths and weaknesses of the available methods to detect and quantify contact sites, with a main focus on ER–mitochondria tethering.
Downregulation of CTRP-3 by Weight Loss In Vivo and by Bile Acids and Incretins in Adipocytes In Vitro
Authors: Schmid Andreas , Gehl Jonas , Thomalla Miriam , Hochberg Alexandra , Kreiß Anja , Patz Marissa , Karrasch Thomas , Schäffler Andreas
Published: November 2020

The adipokine CTRP-3 (C1q/TNF-related protein-3) exerts anti-inflammatory and anti-diabetic effects. Its regulation in obesity and during weight loss is unknown. Serum and adipose tissue (AT) samples were obtained from patients (n = 179) undergoing bariatric surgery (BS). Moreover, patients (n = 131) participating in a low-calorie diet (LCD) program were studied. CTRP 3 levels were quantified by ELISA and mRNA expression was analyzed in AT and in 3T3-L1 adipocytes treated with bile acids and incretins. There was a persistent downregulation of CTRP-3 serum levels during weight loss. CTRP-3 expression was higher in subcutaneous than in visceral AT and serum levels of CTRP-3 were positively related to AT expression levels. A rapid decrease of circulating CTRP-3 was observed immediately upon BS, suggesting weight loss-independent regulatory mechanisms. Adipocytes CTRP-3 expression was inhibited by primary bile acid species and GLP 1. Adipocyte-specific CTRP-3 deficiency increased bile acid receptor expression. Circulating CTRP-3 levels are downregulated during weight loss, with a considerable decline occurring immediately upon BS. Mechanisms dependent and independent of weight loss cause the post-surgical decline of CTRP-3. The data strongly argue for regulatory interrelations of CTRP-3 with bile acids and incretin system.
Platelets, Thrombocytosis, and Ovarian Cancer Prognosis: Surveying the Landscape of the Literature
Authors: Hufnagel Demetra H. , Cozzi Gabriella D. , Crispens Marta A. , Beeghly-Fadiel Alicia
Published: November 2020

Platelets are critical components of a number of physiologic processes, including tissue remodeling after injury, wound healing, and maintenance of vascular integrity. Increasing evidence suggests that platelets may also play important roles in cancer. In ovarian cancer, thrombocytosis, both at the time of initial diagnosis and at recurrence, has been associated with poorer prognosis. This review describes current evidence for associations between thrombocytosis and ovarian cancer prognosis and discusses the clinical relevance of platelet count thresholds and timing of assessment. In addition, we discuss several mechanisms from in vitro, in vivo, and clinical studies that may underlie these associations and recommend potential approaches for novel therapeutic targets for this lethal disease.
5-Nitro-2,4-Dichloropyrimidine as an Universal Model for Low-Energy Electron Processes Relevant for Radiosensitization
Authors: Luxford Thomas F. M. , Pshenichnyuk Stanislav A. , Asfandiarov Nail L. , Perečko Tomáš , Falk Martin , Kočišek Jaroslav
Published: November 2020

We report experimental results of low-energy electron interactions with 5-nitro-2,4-dichloropyrimidine isolated in the gas phase and hydrated in a cluster environment. The molecule exhibits a very rare combination of many so far hypothesized low-energy electron induced mechanisms, which may be responsible for synergism in concurrent chemo-radiation therapy of cancer. In contrast to many previous efforts to design an ideal radiosensitizer based on one mode of action, the present model molecule presents an alternative approach, where several modes of action are combined. With respect to the processes induced by the low-energy electrons, this is not a trivial task because of strong bond specificity of the dissociative electron attachment reaction, as it is discussed in the present paper. Unfortunately, low solubility and high toxicity of the molecule, as obtained from preliminary MTT assay tests, do not enable further studies of its activity in real biological systems but it can advantageously serve as a model or a base for rational design of radiosensitizers.
Inhibiting P2Y12 in Macrophages Induces Endoplasmic Reticulum Stress and Promotes an Anti-Tumoral Phenotype
Authors: Pavlović Nataša , Kopsida Maria , Gerwins Pär , Heindryckx Femke
Published: November 2020

The P2Y12 receptor is an adenosine diphosphate responsive G protein-coupled receptor expressed on the surface of platelets and is the pharmacologic target of several anti-thrombotic agents. In this study, we use liver samples from mice with cirrhosis and hepatocellular carcinoma to show that P2Y12 is expressed by macrophages in the liver. Using in vitro methods, we show that inhibition of P2Y12 with ticagrelor enhances tumor cell phagocytosis by macrophages and induces an anti-tumoral phenotype. Treatment with ticagrelor also increases the expression of several actors of the endoplasmic reticulum (ER) stress pathways, suggesting activation of the unfolded protein response (UPR). Inhibiting the UPR with tauroursodeoxycholic acid (Tudca) diminishes the pro-phagocytotic effect of ticagrelor, thereby indicating that P2Y12 mediates macrophage function through activation of ER stress pathways. This could be relevant in the pathogenesis of chronic liver disease and cancer, as macrophages are considered key players in these inflammation-driven pathologies.
Revealing Alteration in the Hepatic Glucose Metabolism of Genetically Improved Carp, Jayanti Rohu Labeo rohita Fed a High Carbohydrate Diet Using Transcriptome Sequencing
Authors: Rasal Kiran D. , Iquebal Mir Asif , Dixit Sangita , Vasam Manohar , Raza Mustafa , Sahoo Lakshman , Jaiswal Sarika , Nandi Samiran , Mahapatra Kanta Das , Rasal Avinash , Udit Uday Kumar , Meher Prem Kumar , Murmu Khuntia , Angadi UB , Rai Anil , Kumar Dinesh , Sundaray Jitendra Kumar
Published: November 2020

Although feed cost is the greatest concern in aquaculture, the inclusion of carbohydrates in the fish diet, and their assimilation, are still not well understood in aquaculture species. We identified molecular events that occur due to the inclusion of high carbohydrate levels in the diets of genetically improved ‘Jayanti rohu’ Labeo rohita. To reveal transcriptional changes in the liver of rohu, a feeding experiment was conducted with three doses of gelatinized starch (20% (control), 40%, and 60%). Transcriptome sequencing revealed totals of 15,232 (4464 up- and 4343 down-regulated) and 15,360 (4478 up- and 4171 down-regulated) differentially expressed genes. Up-regulated transcripts associated with glucose metabolisms, such as hexokinase, PHK, glycogen synthase and PGK, were found in fish fed diets with high starch levels. Interestingly, a de novo lipogenesis mechanism was found to be enriched in the livers of treated fish due to up-regulated transcripts such as FAS, ACCα, and PPARγ. The insulin signaling pathways with enriched PPAR and mTOR were identified by Kyoto Encyclopedia of Genes and Genome (KEGG) as a result of high carbohydrates. This work revealed for the first time the atypical regulation transcripts associated with glucose metabolism and lipogenesis in the livers of Jayanti rohu due to the inclusion of high carbohydrate levels in the diet. This study also encourages the exploration of early nutritional programming for enhancing glucose efficiency in carp species, for sustainable and cost-effective aquaculture production.
Application of In Vitro Metabolism Activation in High-Throughput Screening
Authors: Ooka Masato , Lynch Caitlin , Xia Menghang
Published: November 2020

In vitro methods which incorporate metabolic capability into the assays allow us to assess the activity of metabolites from their parent compounds. These methods can be applied into high-throughput screening (HTS) platforms, thereby increasing the speed to identify compounds that become active via the metabolism process. HTS was originally used in the pharmaceutical industry and now is also used in academic settings to evaluate biological activity and/or toxicity of chemicals. Although most chemicals are metabolized in our body, many HTS assays lack the capability to determine compound activity via metabolism. To overcome this problem, several in vitro metabolic methods have been applied to an HTS format. In this review, we describe in vitro metabolism methods and their application in HTS assays, as well as discuss the future perspectives of HTS with metabolic activity. Each in vitro metabolism method has advantages and disadvantages. For instance, the S9 mix has a full set of liver metabolic enzymes, but it displays high cytotoxicity in cell-based assays. In vitro metabolism requires liver fractions or the use of other metabolically capable systems, including primary hepatocytes or recombinant enzymes. Several newly developed in vitro metabolic methods, including HepaRG cells, three-dimensional (3D) cell models, and organ-on-a-chip technology, will also be discussed. These newly developed in vitro metabolism approaches offer significant progress in dissecting biological processes, developing drugs, and making toxicology studies quicker and more efficient.
Making the Case for a P2P Personal Health Record
Authors: Horne William Connor , Ben Miled Zina
Published: November 2020

Improved health care services can benefit from a more seamless exchange of medical information between patients and health care providers. This exchange is especially important considering the increasing trends in mobility, comorbidity and outbreaks. However, current Electronic Health Records (EHR) tend to be institution-centric, often leaving the medical information of the patient fragmented and more importantly inaccessible to the patient for sharing with other health providers in a timely manner. Nearly a decade ago, several client–server models for personal health records (PHR) were proposed. The aim of these previous PHRs was to address data fragmentation issues. However, these models were not widely adopted by patients. This paper discusses the need for a new PHR model that can enhance the patient experience by making medical services more accessible. The aims of the proposed model are to (1) help patients maintain a complete lifelong health record, (2) facilitate timely communication and data sharing with health care providers from multiple institutions and (3) promote integration with advanced third-party services (e.g., risk prediction for chronic diseases) that require access to the patient’s health data. The proposed model is based on a Peer-to-Peer (P2P) network as opposed to the client–server architecture of the previous PHR models. This architecture consists of a central index server that manages the network and acts as a mediator, a peer client for patients and providers that allows them to manage health records and connect to the network, and a service client that enables third-party providers to offer services to the patients. This distributed architecture is essential since it promotes ownership of the health record by the patient instead of the health care institution. Moreover, it allows the patient to subscribe to an extended range of personalized e-health services.
Prevalence and Outcomes of Acute Hypoxaemic Respiratory Failure in Wales: The PANDORA-WALES Study
Authors: Kopczynska Maja , Sharif Ben , Pugh Richard , Otahal Igor , Havalda Peter , Groblewski Wojciech , Lynch Ceri , George David , Sutherland Jayne , Pandey Manish , Jones Phillippa , Murdoch Maxene , Hatalyak Adam , Jones Rhidian , Kacmarek Robert , Villar Jesús , Szakmany Tamas , on behalf of the PANDORA-WALES Investigators
Published: November 2020

Background: We aimed to identify the prevalence of acute hypoxaemic respiratory failure (AHRF) in the intensive care unit (ICU) and its associated mortality. The secondary aim was to describe ventilatory management as well as the use of rescue therapies. Methods: Multi-centre prospective study in nine hospitals in Wales, UK, over 2-month periods. All patients admitted to an ICU were screened for AHRF and followed-up until discharge from the ICU. Data were collected from patient charts on patient demographics, clinical characteristics, management and outcomes. Results: Out of 2215 critical care admissions, 886 patients received mechanical ventilation. A total of 197 patients met inclusion criteria and were recruited. Seventy (35.5%) were non-survivors. Non-survivors were significantly older, had higher SOFA scores and received more vasopressor support than survivors. Twenty-five (12.7%) patients who fulfilled the Berlin definition of acute respiratory distress syndrome (ARDS) during the ICU stay without impact on overall survival. Rescue therapies were rarely used. Analysis of ventilation showed that median Vt was 7.1 mL/kg PBW (IQR 5.9–9.1) and 21.3% of patients had optimal ventilation during their ICU stay. Conclusions: One in four mechanically ventilated patients have AHRF. Despite advances of care and better, but not optimal, utilisation of low tidal volume ventilation, mortality remains high.
Associations of HDL Subspecies Defined by ApoC3 with Non-Alcoholic Fatty Liver Disease: The Multi-Ethnic Study of Atherosclerosis
Authors: Morze Jakub , Koch Manja , Aroner Sarah A. , Budoff Matthew , McClelland Robyn L. , Mukamal Kenneth J. , Jensen Majken K.
Published: November 2020

Previously, we reported that inverse associations of high-density lipoprotein (HDL) with cardiovascular disease and diabetes were only observed for HDL that lacked the pro-inflammatory protein apolipoprotein C3 (apoC3). To provide further insight into the cardiometabolic properties of HDL subspecies defined by the presence or absence of apoC3, we aimed to examine these subspecies with liver fat content and non-alcoholic fatty liver disease (NAFLD). We investigated cross-sectional associations between ELISA-measured plasma levels of apoA1 in HDL that contained or lacked apoC3 and computed tomography-determined liver fat content and NAFLD (<51 HU) at baseline (2000–2002) among 5007 participants in the Multi-Ethnic Study of Atherosclerosis (MESA) without heavy alcohol consumption (>14 drinks/week in men and >7 drinks/week in women). In multivariable-adjusted regression models, apoA1 in HDL that contained or lacked apoC3 was differentially associated with liver fat content (Pheterogeneity = 0.048). While apoA1 in HDL that lacked apoC3 was inversely associated with liver fat content (Ptrend < 0.0001), apoA1 in HDL that contained apoC3 was not statistically significantly associated with liver fat content (Ptrend = 0.57). Higher apoA1 in HDL that lacked apoC3 was related to a lower prevalence of NAFLD (OR per SD: 0.80; 95% CI: 0.72, 0.89), whereas no association was found for apoA1 in HDL that contained apoC3 (OR per SD: 0.95; 95% CI: 0.85, 1.05; Pheterogeneity = 0.09). Higher apoA1 in HDL that lacked apoC3 was associated with less liver fat content and a lower prevalence of NAFLD. This finding extends the inverse association of HDL lacking apoC3 from cardiovascular disease to NAFLD. Lack of biopsy-proven hepatic steatosis and fibrosis data requires the replication of our study in further studies.
Effects of Netarsudil on Actin-Driven Cellular Functions in Normal and Glaucomatous Trabecular Meshwork Cells: A Live Imaging Study
Authors: Keller Kate E. , Kopczynski Casey
Published: November 2020

The actin cytoskeleton of trabecular meshwork (TM) cells is a therapeutic target for lowering intraocular pressure (IOP) in glaucoma patients. Netarsudil (the active ingredient in RhopressaTM) is a Rho-associated protein kinase inhibitor that induces disassembly of actin stress fibers. Here, we used live cell imaging of SiR-actin-labeled normal (NTM) and glaucomatous TM (GTM) cells to investigate actin dynamics during actin-driven biological processes with and without netarsudil treatment. Actin stress fibers were thicker in GTM than NTM cells and took longer (>120 min) to disassemble following addition of 1 µM netarsudil. Actin-rich extracellular vesicles (EVs) were derived by two mechanisms: exocytosis of intracellular-derived vesicles, and cleavage of filopodial tips, which detached the filopodia from the substratum, allowing them to retract to the cell body. While some phagocytosis was noted in untreated TM cells, netarsudil potently stimulated phagocytic uptake of EVs. Netarsudil treatment induced lateral fusion of tunneling nanotubes (TNTs) that connected adjacent TM cells; TNTs are important for TM cellular communication. Together, our results suggest that netarsudil may clear outflow channels in TM tissue by inducing phagocytosis and/or by modulating TM communication via EVs and TNTs. These cellular functions likely work together to regulate IOP in normal and glaucomatous TM.
Fetal and Placental Weight in Pre-Gestational Maternal Obesity (PGMO) vs. Excessive Gestational Weight Gain (EGWG)—A Preliminary Approach to the Perinatal Outcomes in Diet-Controlled Gestational Diabetes Mellitus
Authors: Gujski Mariusz , Szukiewicz Dariusz , Chołuj Marta , Sawicki Włodzimierz , Bojar Iwona
Published: November 2020

Both pre-gestational maternal obesity (PGMO) and excessive gestational weight gain (EGWG) increase the risk of gestational diabetes mellitus (GDM). Here, we conducted a retrospective study to comparatively examine the relation between fetal birth weight (FW) and placental weight (PW) in PGMO (n = 100) compared to EGWG (n = 100) with respect to perinatal outcomes in diet-controlled GDM. The control group was made up of 100 healthy pregnancies. The mean FW and the mean PW in EGWG were correlated with lowered fetal weight/placental weight ratio (FW/PW ratio). The percentage of births completed by cesarean section accounted for 47%, 32%, and 18% of all deliveries (EGWG, PGMO, and controls, respectively), with the predominance of FW-related indications for cesarean section. Extended postpartum hospital stays due to neonate were more frequent in EGWG, especially due to neonatal jaundice (p < 0.05). The results indicate the higher perinatal risk in mothers with EGWG compared to PGMO during GDM-complicated pregnancy. Further in-depth comparative studies involving larger patient pools are needed to validate these findings, the intent of which is to formulate guidelines for GDM patients in respect to management of PGMO and EGWG.
Patients with Inflammatory Bowel Disease Are Not at Increased Risk of COVID-19: A Large Multinational Cohort Study
Authors: Allocca Mariangela , Chaparro María , Gonzalez Haidee Aleman , Bosca-Watts Marta Maia , Palmela Carolina , D’Amico Ferdinando , Zacharopoulou Eirini , Kopylov Uri , Ellul Pierre , Bamias Giorgos , Ntelis Vassilios , Lahat Adi , Mantzaris Gerassimos J , Papaconstantinou Ioannis , Katsanos Konstantinos , Uspenskaya Yulia , Christodoulou Dimitrios , Ben Horin Shomron , Peyrin-Biroulet Laurent , Torres Joanna , Sebastian Shaji , Gisbert Javier P , Danese Silvio , Fiorino Gionata
Published: November 2020

The impact of COVID-19 on inflammatory bowel disease (IBD) patients under pharmacological immunosuppression is still not clearly understood. We investigated the incidence of COVID-19 and the impact of immunosuppression and containment measures on the risk of SARS-CoV-2 infection in a large IBD cohort, from a multicenter cohort from 21st of February to 30th of June, 2020. Ninety-seven patients with IBD (43 UC, 53 CD, one unclassified IBD) and concomitant COVID-19 over a total of 23,879 patients with IBD were enrolled in the study. The cumulative incidence of SARS-CoV-2 infection in patients with IBD vs. the general population was 0.406% and 0.402% cases, respectively. Twenty-three patients (24%) were hospitalized, 21 (22%) had pneumonia, four (4%) were admitted to the Intensive Care Unit, and one patient died. Lethality in our cohort was 1% compared to 9% in the general population. At multivariable analysis, age > 65 years was associated with increased risk of pneumonia and hospitalization (OR 11.6, 95% CI 2.18–62.60; OR 5.1, 95% CI 1.10–23.86, respectively), treatment with corticosteroids increased the risk of hospitalization (OR 7.6, 95% CI 1.48–40.05), whereas monoclonal antibodies were associated with reduced risk of pneumonia and hospitalization (OR 0.1, 95% CI 0.04–0.52; OR 0.3, 95% CI 0.10–0.90, respectively). The risk of COVID-19 in patients with IBD is similar to the general population. National lockdown was effective in preventing infection in our cohort. Advanced age and treatment with corticosteroids impacted negatively on the outcome of COVID-19, whereas monoclonal antibodies did not seem to have a detrimental effect.
Sediment Deposition Following Construction of a Breakwater Harbor: Point Judith Harbor of Refuge, Rhode Island, USA
Authors: Oakley Bryan A. , Murphy Cody J. , Lee Kym K. , Hollis Robert J. , Caccioppoli Brian , King John W.
Published: November 2020

Breakwaters are a common shoreline protection structure, often trapping sediment as the incoming wave energy is reduced. Quantifying the dynamics and volume of these sediment sinks within a coastal system is an important step toward understanding the sediment budget for a particular coastal area. This study examines the volume of sediment deposited within the breakwater enclosed Point Judith Harbor of Refuge (Rhode Island, United States of America (USA)) in the late 19th century using seismic reflection profiles, bathymetric mapping, and isotopic analysis of core sediment. Geophysical profiles show a district seismic facies up to 4 m thick above the ravinement surface, particularly in the western and central portion of the harbor. Century-scale bathymetric changes revealed shoaling of a similar magnitude, and isotopic data support the deposition of this sediment package within the 20th century. The total volume of sediment within the harbor exceeds 5.0 × 106 m3, with an estimated sand volume of 3.6 × 106 m3. The results show that the harbor is a substantial sediment sink for the Rhode Island South Shore and provide the basis for future studies of the sediment budget for this shoreline.
Cross-Shore Intertidal Bar Behavior along the Dutch Coast: Laser Measurements and Conceptual Model
Authors: Vos Sander , Spaans Lennard , Reniers Ad , Holman Rob , Mccall Robert , de Vries Sierd
Published: November 2020

Intertidal bars are naturally occurring morphological features along the waterline of sandy beaches. Present quantitative knowledge on intertidal bar behavior is limited, due to the scarcity of data resources and the limitations of traditional survey techniques. To investigate and quantify the cross-shore morphologic behavior of intertidal bars, hourly terrestrial laser scans of Kijkduin beach (The Netherlands) are used and a conceptual evolution intertidal bar model is constructed. In a six-week period in January and February 2017, a pronounced intertidal bar formed at Kijkduin beach and migrated onshore during mild wave conditions and eroded again during storm conditions. The observed maximum shoreward migration was 30 m horizontally with a maximum growth of about 1 m in the vertical direction. Onshore sediment transport fluxes peaked around 2 m3 per m width per day. In the conceptual model proposed here, run-up and overwash processes are dominant for shoreward growth and migration of the bar and submersion processes are responsible for bar destruction.
Geometrical Analysis of the Inland Topography to Assess the Likely Response of Wave-Dominated Coastline to Sea Level: Application to Great Britain
Authors: Payo Andres , Williams Chris , Vernon Rowan , Hulbert Andrew , Lee Kathryn , Lee Jonathan
Published: November 2020

The need for quantitative assessments at a large spatial scale (103 km) and over time horizons of the order 101 to 102 years have been reinforced by the 2019 Special Report on the Ocean and Cryosphere in a Changing Climate, which concluded that adaptation to a sea-level rise will be needed no matter what emission scenario is followed. Here, we used a simple geometrical analysis of the backshore topography to assess the likely response of any wave-dominated coastline to a sea-level rise, and we applied it along the entire Great Britain (GB) coastline, which is ca. 17,820 km long. We illustrated how the backshore geometry can be linked to the shoreline response (rate of change and net response: erosion or accretion) to a sea-level rise by using a generalized shoreline Exner equation, which includes the effect of the backshore slope and differences in sediment fractions within the nearshore. To apply this to the whole of GB, we developed an automated delineation approach to extract the main geometrical attributes. Our analysis suggests that 71% of the coast of GB is best described as gentle coast, including estuarine coastline or open coasts where back-barrier beaches can form. The remaining 29% is best described as cliff-type coastlines, for which the majority (57%) of the backshore slope values are negative, suggesting that a non-equilibrium trajectory will most likely be followed as a response to a rise in sea level. For the remaining 43% of the cliffed coast, we have provided regional statistics showing where the potential sinks and sources of sediment are likely to be.
Applicability of the Goda–Takahashi Wave Load Formula for Vertical Slender Hydraulic Structures
Authors: Meinen Nadieh Elisabeth , Steenbergen Raphaël Daniël Johannes Maria , Hofland Bas , Jonkman Sebastiaan Nicolaas
Published: November 2020

Vertical slender hydraulic structures such as sluices, navigation locks, or storm-surge barriers are often dynamically loaded by waves. For a safe and economic design, an accurate description of the wave loads is needed. A widely used formula for this purpose is the Goda–Takahashi wave load formula (GT). It was derived for the assessment of gravity-based caisson breakwaters. Due to its many advantages, the formula is also often employed for the assessment of vertical slender hydraulic structures, although its applicability to those type of structures was never fully demonstrated. This study provides insights in the applicability of GT for vertical slender hydraulic structures. This is done based on a literature review on the historical backgrounds of GT, and an investigation of several case-studies. In the case-studies, the equivalent-static wave loads for caisson breakwaters in scope of GT are compared with those for vertical slender hydraulic structures. The results show that GT can safely be applied for vertical slender hydraulic structures loaded by pulsating wave loads, but that systematic over- or under-estimations are expected for breaking or impact wave loads. For individual cases, differences up to 200% were obtained. These large over- or under-estimations underline the need for an improvement of the current design tools for vertical slender hydraulic structures loaded by breaking or impact wave loads.
Analysis of miRNA Expression in Patients with Rheumatoid Arthritis during Olokizumab Treatment
Authors: Bure Irina V. , Mikhaylenko Dmitry S. , Kuznetsova Ekaterina B. , Alekseeva Ekaterina A. , Bondareva Kristina I. , Kalinkin Alexey I. , Lukashev Alexander N. , Tarasov Vadim V. , Zamyatnin Andrey A. , Nemtsova Marina V.
Published: November 2020

Rheumatoid arthritis (RA) is the most common autoimmune disease worldwide. Epigenetic alternations of microRNAs (miRNAs) can contribute to its pathogenesis and progression. As the first line therapy with DMARDs is not always successful, other drugs and therapeutic targets should be applied. This study aims to measure the expression level of plasma miRNAs in RA patients treated with olokizumab and to evaluate their potential as prognostic biomarkers. The expression of 9 miRNAs was quantified in 103 RA patients before treatment and at weeks 12 and 24 of olokizumab therapy by reverse transcription-polymerase chain reaction (RT-PCR) assay and analyzed in groups of responders and non-responders. Almost all miRNAs changed their expression during therapy. The ROC curve analysis of the most prominent of them together with consequent univariate and multivariate regression analysis revealed statistically significant associations with the olokizumab therapy efficiency scores for miR-26b, miR-29, miR-451, and miR-522. Therefore, these miRNAs might be a potential therapeutic response biomarker.
Mobilizing Indigenous Knowledge through the Caribou Hunter Success Working Group
Authors: Atkinson Hannah
Published: November 2020

The caribou stewardship practices of the Iñupiat have persisted through cycles of abundance and decline for the Western Arctic Caribou Herd (WACH). This research seeks to address the challenges and opportunities faced when mobilizing Indigenous Knowledge in the National Park Service (NPS) management of the herd. Motivated by Indigenous stewardship concerns, NPS staff facilitate and participate in an informal working group focused on caribou hunter success. Using Indigenous Knowledge methods, this study examined the outcomes of the working group and the use of “rules of thumb” to identify and share stewardship practices. In the two cases, the Caribou Hunter Success Working Group created space for subsistence hunters to develop educational materials based on Indigenous Knowledge to address specific hunter success issues. Subsistence users participate in the federal subsistence programs and related subsistence forums, and it is the work of the NPS to mobilize the knowledge they contribute to improve subsistence management for both the users and the resource. There are two additional benefits for the NPS: (1) a better understanding of the use of the resource, and (2) when regulations are informed by Indigenous Knowledge, there is a greater likelihood of adherence. The mobilization of Indigenous Knowledge leads to more effective management.
Knowledge Mobilization in the Beaver Hills Biosphere, Alberta, Canada
Authors: Blye Clara-Jane , Halpenny Elizabeth A. , Hvenegaard Glen T. , Patriquin Dee
Published: November 2020

This study explores how knowledge was and is mobilized to advance the objectives of the Beaver Hills Biosphere Reserve, located in Alberta, Canada. Established in 2016, a 12-year collaborative effort worked to establish the biosphere reserve and achieve formal UNESCO designation. Subsequent efforts to grow the newly established biosphere reserve have accelerated in recent years. Our study documented how different types of knowledge were accessed, created, curated, and shared between partners during these two time periods. Focus group interviews were conducted with 14 participants, who are affiliated with Beaver Hills Biosphere Reserve partner organizations, and revealed the following findings: (1) not all knowledge is equally valued or understood; (2) partnerships are highly valued, and were essential to successful knowledge mobilization, but were stronger among individuals rather than organizations; (3) fear of the loss of autonomy and potential complications due to the establishment of a biosphere reserve slowed the exchange of information and engagement by some regional actors; and (4) knowledge mobilization is and was impeded by staff and agency capacity, finances, and time scarcity. This was further complicated by entrenched norms of practice, existing successful working relationships impeding the development of new partnerships, and embracing alternative forms of knowledge.
Synthesis, Electrochemical Studies, and Antimicrobial Properties of Fe3O4 Nanoparticles from Callistemon viminalis Plant Extracts
Authors: Uwaya Gloria E. , Fayemi Omolola E. , Sherif El-Sayed M. , Junaedi Harri , Ebenso Eno E.
Published: November 2020

Less toxic, environmentally safe green-mediated iron (III) oxide nanoparticles (Fe3O4-NP) synthesized using Callistemon viminalis (C. viminalis) leaf (Fe3O4-NPL) and flower (Fe3O4-NPF) extracts is reported in this work for the first time. Total flavonoids and phenols present in the plant extracts were determined. Characterization of the nanoparticles was carried out using Fourier transform infrared (FTIR) spectroscopy, ultraviolet–visible spectroscopy (UV–VIS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and Malvern zeta sizer. Other properties of the nanoparticles were investigated using the thermogravimetric analyser and cyclic voltammetry. The average particle sizes obtained for Fe3O4-NPL and Fe3O4-NPF were 17.91 nm and 27.93 nm, respectively. Fe3O4-NPL exhibited an excellent electrochemical activity when compared with Fe3O4-NPF based on a stability study using cyclic voltammetry and regression value. Additionally, Fe3O4-NPF displayed excellent antimicrobial activity against Bacillus cereus, Salmonella enteritidis, and Vibrio cholerae with zones of inhibition of 13, 15, and 25 mm, respectively. Simple, cheap, and less toxic green-mediated iron (III) oxide nanoparticles synthesized from C. viminalis leaf (Fe3O4-NPL) and flower (Fe3O4-NPF) extracts hold the potential of being used to control the activity of pathogenic bacteria of health importance and as an electrochemical sensor for both biological and environmental analytes.
Effect of Silane Coupling Agent on Modification of Areca Fiber/Natural Latex
Authors: Pan Yiren , Zhang Meng , Zhang Jian , Zhu Xiaoyao , Bian Huiguang , Wang Chuansheng
Published: November 2020

In this paper, the areca fiber was extracted by physical and chemical treatment, and then the areca fiber/natural latex composite was prepared by natural latex impregnation technology. In order to combine areca fiber and natural rubber latex better, three silane coupling agents with different action mechanism were selected: Si−69, KH550, and KH570 which were used to treat the areca fiber/natural latex compound. The results show that the silane coupling agent can change the surface of the fiber from hydrophilic surface to organophilic surface, making the bonding of areca fiber to natural latex more closely. At the same time, the mechanical properties, physical and mechanical properties, swelling properties, and dynamic viscoelastic properties of the tightly bonded areca fiber/nature latex composites were improved. After observing the micro-structure through a scanning electron microscope, it was found that the three silane coupling agents could effectively bind areca fiber and natural latex to enhance the performance of the composite material, of which Si−69 performed best, and the tensile strength and tear strength of the composite increased by 21.19% and 12.90% respectively.
Evaluation of Osteoconduction of a Synthetic Hydroxyapatite/β-Tricalcium Phosphate Block Fixed in Rabbit Mandibles
Authors: Pires Luis Carlos de Almeida , da Silva Rodrigo Capalbo , Poli Pier Paolo , Ruas Esgalha Fernando , Hadad Henrique , Palin Letícia Pitol , Piquera Santos Ana Flávia , Teixiera Colombo Luara , Kawamata de Jesus Laís , Bassi Ana Paula Farnezi , Maiorana Carlo , Okamoto Roberta , de Carvalho Paulo Sérgio Perri , Souza Francisley Ávila
Published: November 2020

(1) Background: This study aimed to evaluate the incorporation of hydroxyapatite/β-tricalcium phosphate blocks grafted in rabbit mandibles. (2) Methods: Topographic characterization of biomaterial was performed through scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM-EDX). Ten rabbits randomly received autogenous bone graft harvested from the tibia (Autogenous Group—AG) or synthetic biomaterial manufactured in β-tricalcium phosphate (Biomaterial Group—BG) at their right and left mandibular angles. Euthanasia was performed at 30 and 60 postoperative days; (3) Results: SEM-EDX showed a surface with the formation of crystals clusters. Histological analyses in BG at 30 days showed a slower process of incorporation than AG. At 60 days, BG showed remnants of biomaterial enveloped by bone tissue in the anabolic modeling phase. Histometric analysis showed that mean values of newly formed bone-like tissue in the AG (6.56%/9.70%) were statistically higher compared to BG (3.14%/6.43%) in both periods, respectively. Immunohistochemical analysis demonstrated early bone formation and maturation in the AG with more intense osteopontin and osteocalcin staining. (4) Conclusions: The biomaterial proved to be a possible bone substitute, being incorporated into the receiving bed; however, it showed delayed bone incorporation compared to autogenous bone.
Anti-Oxidative Activity of Alcohol-Water Extracts from Field Horsetail (Equisteum arvense) in Elastomer Vulcanizates Subjected to Accelerated Aging Processes
Authors: Masłowski Marcin , Miedzianowska Justyna , Czylkowska Agnieszka , Efenberger-Szmechtyk Magdalena , Nowak Agnieszka , Strzelec Krzysztof
Published: November 2020

The article aimed to highlight the antioxidant potential of natural extracts obtained from Equisteum arvense in elastomer vulcanizatec made of natural rubber. Horsetail extracts were prepared using four solvent systems at different volume ratios: methanol–water (50/50 and 70/30) and ethanol–water (50/50 and 70/30), which were then lyophilized and added to the polymer matrix. A deep characterization of the obtained bio-additives was performed. The obtained vulcanizates were subjected to various aging processes: thermo-oxidative, ultraviolet and solar. Then, the resistance and susceptibility of vulcanizates to selected processes of accelerated, simulated degradation were determined based on the changes in the spatial structure (network nodes), material strength and physical properties (color). The research showed the presence of polyphenols in the extracts of horsetail, which resulted in their high anti-oxidative activity. Moreover, the extracts were able to absorb UV radiation. As a consequence, they were active additives that protected rubber vulcanizates against the damaging effects of the aging processes, securing their functional properties. Samples containing natural extracts were characterized by better stability of: mechanical strength parameters, the increase in cross-linking density and color changes after simulating aging processes in comparison with the control sample.
Effect of Aromatic System Expansion on Crystal Structures of 1,2,5-Thia- and 1,2,5-Selenadiazoles and Their Quaternary Salts: Synthesis, Structure, and Spectroscopic Properties
Authors: Alfuth Jan , Zadykowicz Beata , Sikorski Artur , Połoński Tadeusz , Eichstaedt Katarzyna , Olszewska Teresa
Published: November 2020

Rational manipulation of secondary bonding interactions is a crucial factor in the construction of new chalcogenadiazole-based materials. This article reports detailed experimental studies on phenanthro[9,10-c][1,2,5]chalcogenadiazolium and 2,1,3-benzochalcogenadiazolium salts and their precursors. The compounds were synthesized, characterized employing NMR and UV-Vis spectroscopy. TD-DFT calculations were also performed. The influence of the size of the aromatic system on the molecular motifs formed by the compounds in the solid state has been studied by means of single-crystal X-ray diffraction. In case of the salts, the nature of an anion was also taken into consideration. The results show that cyclic [E···N]2 supramolecular synthon connects neighboring molecules of phenanthro[9,10-c][1,2,5]chalcogenadiazoles, with a relatively large aromatic system, in dimers regardless of the chalcogen atom in the molecule. Both N-methyl-2,1,3-benzothiadiazolium and N-methylphenanthro[9,10-c][1,2,5]chalcogenadiazolium cations have a strong affinity for triflate and iodide anions, therefore the formation of S···N or Se···N secondary bonding interactions is observed only in two out of the eight quaternary salts. Less coordinating anions must be used to enable the building blocks studied to form cyclic [E···N]2 synthons. Moreover, for two of the triflate salts, which are isostructural, a new supramolecular motif has been observed.
Significant Improvement the Mechanical Properties of CoCrNi Alloy by Tailoring a Dual FCC-Phase Structure
Authors: Yi Hailong , Bi Mengyuan , Yang Kang , Zhang Bing
Published: November 2020

In this work, SixCoCrNi (x = 0, 0.1, 0.2, 0.3, 0.4, molar ratio) alloys were designed by introducing elemental Si into CoCrNi, a medium-entropy alloy (MEA). The effects of Si addition on the microstructure and mechanical properties of the as-cast SiCoCrNi alloys were investigated. The results suggested that a novel Si-rich face-centred cubic (FCC) phase formed in the Si0.3CoCrNi and Si0.4CoCrNi alloys. The Si-rich FCC phase, which featured high hardness and was located between the dendrites of the matrix FCC phase (with higher phase fraction), significantly increased the yield strength of the Si0.4CoCrNi alloys five times compared with the CoCrNi alloy.