bluuebunny/crossref_metadata_2025 huggingface dataset:
Amantadine as an Aid to Extubation in Severe Acute Brain Injury: A Case Series
Authors: Fang Benjamin , Angulo Castro Sergio , McHugh Daryl C.
Published: February 2024

For a subset of patients with severe acute brain injury (SABI) undergoing invasive mechanical ventilation, the primary barrier to successful extubation is depressed mental status. Amantadine is a neurostimulant that has been demonstrated to increase arousal and improve functional outcomes in patients with SABI. In this case series, we describe 5 patients with SABI and invasive mechanical ventilation who received amantadine as an agent to improve mental status to allow extubation. The primary barrier to extubation for all patients was depressed mental status. Median age was 77 (range 32 to 82). Primary diagnoses were ischemic stroke (n = 1), subdural hemorrhage (n = 2), intracerebral hemorrhage (n = 1), and traumatic brain injury (n = 1). Median Glasgow Coma Score was 7T prior to administration of amantadine and 10T on the day after amantadine was initiated, with improvements in eye-opening and motor response. Four patients displayed improvement in arousal and attention and were successfully extubated 1 to 4 days after initiation of amantadine (median 2 days). The fifth patient only displayed marginal improvement in mental status after starting amantadine, but was ultimately able to be extubated 7 days later. Amantadine may improve the likelihood of or reduce the time to successful extubation in patients with SABI.
Liver Biopsy Handling of Metabolic-Associated Fatty Liver Disease (MAFLD): the Children’s Hospital of Eastern Ontario grossing protocol
Authors: Sergi Consolato M. , Kehar Mohit , Jimenez-Rivera Carolina
Published: February 2024

Metabolic-(non-alcoholic) associated fatty liver disease (MAFLD/NAFLD) has increasingly become a worldwide epidemic. It has been suggested that renaming NAFLD to MAFLD is critical in identifying patients with advanced fibrosis and poor cardiovascular outcomes. There are concerns that the progression to non-alcoholic steatohepatitis (NASH) may become a constant drive in the future healthcare of children and adolescents. There is a necessity to tackle the emerging risk factors for NASH-associated hepatocellular carcinoma (HCC). In this narrative review, we present the current protocol of liver biopsy separated between pre-analytical, analytical, and post-analytical handling. Genetic association investigations have identified single nucleotide polymorphisms implicated in the progression of MAFLD-HCC, many of which seem to belong to the lipid metabolism pathways. PNPLA3 rs738409 variant, TM6SF2 rs58542926 variant, MBOAT7 rs641738 variant, and GCKR variants seem to be significantly associated with NAFLD disease susceptibility. In disclosing the current comprehensive protocol performed at the Children’s Hospital of Eastern Ontario, Ottawa, ON, Canada, we support the most recent Kulkarni-Sarin’s pledge to rename NAFLD to MAFLD. Grossing of the liver biopsy is key to identifying histologic, immunophenotypical, and ultrastructure data and properly preserving tissue for molecular genomics data.
Defective blastogenesis of postnatally diagnosed type VI sirenomelia in a young primigravida: A case report
Authors: Albitar Mhd Mustafa , Almouallem Mohamad Moamen , Kanaan Ahmad Mostafa , Alawad Ieman
Published: February 2024

Sirenomelia is a rare lethal multi-systemic birth malformation in which the two lower limbs are replaced with a rotated single midline tail-like limb. Several hypotheses try to explain this syndrome, with the most prominent theories being the “vascular steal hypothesis” and the “defective blastogenesis hypothesis.” We report a case of a baby with sirenomelia who had a single femur and a single tibia, which classify the case as type VI on Stocker and Heifetz classification. The only risk factor in our case is young maternal age. The baby had a single umbilical artery, a prominent feature of the vascular steal hypothesis. Nonetheless, it also had upper limb deformity, which can be better explained by the defective blastogenesis hypothesis. Our case supports the defective blastogenesis theory of sirenomelia more than the vascular steal hypothesis as it has both a single umbilical artery and upper limb deformity. Also, our case serves as a teaching lesson that indicates the importance of an obstetric ultrasound before a cesarean section has to be done to avoid unnecessary surgery for life incompatible congenital anomaly.
Rethinking use-restricted open-source licenses for regulating abuse of generative models
Authors: Cui Jonathan , Araujo David A
Published: February 2024

The rapid progress of Artificial intelligence in generative modeling is marred by widespread misuse. In response, researchers turn to use-based restrictions—contractual terms prohibiting certain uses—as a “solution” for abuse. While these restrictions can be beneficial to artificial intelligence governance in API-gated settings, their failings are especially significant in open-source models: not only do they lack any means of enforcement, but they also perpetuate the current proliferation of tokenistic efforts toward ethical artificial intelligence. This observation echoes growing literature that points to useless efforts in “AI ethics,” and underscores the need to shift from this paradigm. This article provides an overview of these drawbacks and argues that researchers should divert their efforts to studying deployable, effective, and theoretically grounded solutions like watermarking and model alignment from human feedback to effect tangible changes in the current climate of artificial intelligence.
Cystoscopic-guided laser ablation of intramural ectopic ureters in a female cat
Authors: Gibbs Nicole H , Adams Larry G , Murakami Masahiro , Commons Julie , Steinbach Sarah ML
Published: February 2024

Case summary

An 8-month-old female spayed domestic shorthair cat was presented for chronic urinary incontinence (UI). Since being adopted 6 months earlier, the cat had a history of urine leakage during both activity and rest. Baseline blood work and urine culture showed no significant abnormalities and no evidence of a urinary tract infection. An abdominal CT with excretory urography followed by a focal urinary tract ultrasound revealed a suspected right intramural ectopic ureter (EU) and potential left EU. Cystoscopy confirmed bilateral intramural EUs. Cystoscopic-guided laser ablation (CLA) of both EUs was performed. The cat developed temporary urinary obstruction (UO) 36 h after the procedure, which was medically managed with prazosin and buprenorphine. Ultimately, the cat’s urinary signs completely resolved with no UI recognized after the procedure and the cat has remained continent during 18 months of follow-up.

Relevance and novel information

CLA of intramural EUs is routinely performed in dogs, but this technique has not been previously reported in cats with this condition. Although post-procedural urinary tract signs were initially present, the cat ultimately had an excellent outcome with resolution of UI after this procedure.
Resolution of Radiculopathy Following Indirect Versus Direct Decompression in Single Level Lumbar Fusion
Authors: Walia Arnaav , Ani Fares , Maglaras Constance , Raman Tina , Fischer Charla
Published: February 2024

Study Design

Retrospective analysis.

Objectives

To evaluate resolution of radiculopathy in one-level lumbar fusion with indirect or direct decompression techniques.

Methods

Patients ≥18 years of age with preoperative radiculopathy undergoing single-level lumbar fusion with up to 2-year follow-up were grouped by indirect and direct decompression. Direct decompression (DD) group included ALIF and LLIF with posterior DD procedure as well as all TLIF. Indirect decompression (ID) group included ALIF and LLIF without posterior DD procedure. Propensity score matching was used to control for intergroup differences in age. Intergroup outcomes were compared using means comparison tests. Logistic regressions were used to correlate decompression type with symptom resolution over time. Significance set at P < .05.

Results

116 patients were included: 58 direct decompression (DD) (mean 53.9y, 67.2% female) and 58 indirect decompression (ID) (mean 54.6y, 61.4% female). DD patients experienced greater blood loss than ID. Additionally, DD patients were 4.7 times more likely than ID patients to experience full resolution of radiculopathy at 3 months post-op. By 6 months, DD patients demonstrated larger reductions in VAS score. With regard to motor function, DD patients had improved motor score associated with the L5 dermatome at 6 months relative to ID patients.

Conclusions

Direct decompression was associated with greater resolution of radiculopathy in the near post-operative term, with no differences at long term follow-up when compared with indirect decompression. In particularly debilitated patients, these findings may influence surgeons to perform a direct decompression to achieve more rapid resolution of radiculopathy symptoms.
Unpacking the Role of Green Smart Technologies Adoption, Green Ambidextrous Leadership, and Green Innovation Behaviour on Green Innovation Performance in Malaysian Manufacturing Companies
Authors: Hossain Mohammad Imtiaz , Teh Boon Heng , Tabash Mosab I. , Chong Lee-Lee , Ong Tze San
Published: February 2024

Integrating the natural resource-based view theory, contingency theory and paradox theory, the current study investigates the impact of the green smart technologies adoption (GSTA) and green ambidextrous leadership (GAL) on green innovation performance (GIP), along with the mediating role of Green Innovation Behaviour (GIB). The research design for this study consists of a quantitative method, a positivism paradigm, a cross-sectional time horizon and a structured questionnaire survey. A total of 313 responses from Malaysian manufacturing firms were analysed using partial least squares-structural equation modelling (PLS-SEM). The findings reveal that GSTA, GAL and GIB positively impact GIP. Moreover, GIB mediates the association between GSTA and GIP, but there is no mediation observed in the GAL–GIP association. The results of this study provide valuable insights for policymakers, management and relevant stakeholders in developing countries on how to strike the right balance of exploitation and exploration leadership in uncertain and volatile market settings, while fostering GSTA and GIP. The adoption of GSTA and GAL improves manufacturing companies’ sustainability. Furthermore, these findings contribute to the Malaysian government’s commitment to the Nationally Determined Contribution towards the Paris Agreement, the Shared Prosperity 2030 vision and the proposed 15 key economic growth activities.
French Version of the Short Anterior Cruciate Ligament–Return to Sport After Injury Scale: Development and Validation
Authors: Abid Hichem , Rougereau Gregoire , Bohu Yoann , Meyer Alain , Gerometta Antoine , Grimaud Olivier , Lefevre Nicolas , Hardy Alexandre
Published: February 2024

Background:

The 12-item Anterior Cruciate Ligament–Return to Sport after Injury (ACL-RSI) scale was developed to assess the psychological readiness of patients to return to sports after ACL reconstruction (ACLR). A short (6-item) English version was also developed, which has shown to have good reliability and validity.

Purpose/Hypothesis:

We aimed to develop and validate a French version of the short ACL-RSI scale. We hypothesized that the same questions would remain in the selection as the English version and that the French version of the scale would have the same psychometric properties.

Study Design:

Cohort study (diagnosis); Level of evidence, 3.

Methods:

The full 12-item French ACL-RSI scale was administered to 1000 patients who had undergone ACLR surgery. Reliability (Cronbach alpha) and factor analysis of the full scale were determined. Item selection and elimination process was conducted to develop a short (6-item) version. The same methodology was used to develop the English short ACL-RSI scale. A minimal (3-item) version was also developed and assessed.

Results:

Internal consistency of the full version of the French ACL-RSI was found to be high (Cronbach alpha = .95), suggesting item redundancy. The short (6-item) version was also found to have high internal consistency (Cronbach alpha = .92) and was strongly correlated with the full version ( r = 0.98). The minimal (3-item) version was also found to have high internal consistency, as well as a strong correlation with the full version ( r = 0.94).

Conclusion:

The French version of the short (6-item) ACL-RSI scale was valid, discriminant, consistent, and reproducible. The minimal (3-item) version was also found to be useful and more efficient to collect the information provided by the full ACL-RSI in a French-speaking population.
Trends in Meniscus-Related Publications in PubMed Since 1928: A Bibliometric Study
Authors: Kambhampati Srinivas B.S. , D’Ambrosi Riccardo , Vishwanathan Karthik , Vaish Abhishek , Vaishya Raju
Published: February 2024

Background:

As information on the meniscus accumulates in PubMed, it is possible to evaluate the trends in research on the topic over time.

Purpose:

To evaluate the major trends in meniscus-related publications in PubMed from the earliest publication to the present day.

Study Design:

Cross-sectional study.

Methods:

PubMed was searched on January 3, 2023, using the search strategy “menisc *”[All Fields] AND (“knee”[MeSH Terms] OR “knee”[All Fields] OR “knee joint”[MeSH Terms] OR (“knee”[All Fields] AND “joint”[All Fields]) OR “knee joint”[All Fields]) AND 1900/01/01:2022/12/31[Date - Publication]. This retrieved 15,569 human and animal studies. We determined the top 10 meniscus-related publications in terms of overall citations and citations per year, as well as the top 10 authors, journals, countries, and institutions of cited publications on the meniscus. In addition, we performed word-cloud analyses based on meniscus-related terms from different periods (before 1981, before 2000, after 2005, and in the past 5 years [2018-2022]), including the year of first appearance and the number of publications featured.

Results:

Since the first recorded publication on the meniscus in 1928, there was a steady growth in the number of articles until 2005, when there was an upsurge in publications from 254 in 2005 to 955 in 2022. Noyes was the author with the most citations (n = 3314), and a 2006 study by Caplan and Dennis had the most citations per year (n = 125). Arthroscopy published the most articles on the meniscus with 1118, whereas the Journal of Cellular Biochemistry had the most citations per article with 125.69. The most published countries and institutes were the United States and the Hospital for Special Surgery, respectively. Word-cloud analysis of article titles showed that “meniscectomy” had become less prominent, and “root,”“ramp,”“transplantation,” and “slope” had become more prominent.

Conclusion:

There has been an upsurge in publications on the meniscus since 2005, with word-cloud analysis indicating shifting interests in meniscus-related research.
Towards Conceptualizing a Policy and Promoting Advocacy for People With Color Vision Deficiency: Addressing the Importance of Color Vision Deficiency in India and Creating a Global Reference Template
Authors: Male Shiva Ram , Shamanna Bindiganavale Ramaswamy , Gandhi Rashmin
Published: February 2024

Color vision is crucial for daily life, and conditions such as color blindness and deficiencies can negatively impact one's quality of life and livelihood. According to a study in India there was an increase in the prevalence of color vision deficiency (CVD) among children and young adult teenagers, who are losing empowering opportunities in education and academics. However, this trend can be managed with evidence-based new interventions and objectives from India's developing nation models. This policy paper mainly highlights the need for effective strategies to address this issue. Early identification of CVD is crucial for effective interventions and rehabilitation to reduce medical, behavioral, and economic burdens on patients, families, and society. Strategies include policy prescriptions, advocacy, public health campaigns, investing in a trained workforce, and using technology to facilitate early screening in educational institutes. These strategies aim to address the growing healthcare crisis of CVD.
Effect of Nitro Counseling on Lifestyle of Obese Adolescence
Authors: Shouli Mustafa Mohammad , Shouli Khaila Mohammad , Aqtam Ibrahim , Ayed Ahmad
Published: February 2024

Introduction

Obesity during adolescence represents a strong predictor of higher mortality in adulthood; thus, lifestyle interventions represent the recommended therapy.

Objective

To evaluate the effect of nitro counseling on lifestyle of obese adolescence, and develop life changing program.

Method

Quasi-experimental design was conducted between October 2021 and January 2022. A total of 117 university nursing students participated from two universities in Palestine. A self-reported questionnaire was used for data collection and included the following: socio-demographic characteristics, university students’ knowledge, practices adolescent lifestyle, and anthropometric measurement to detect body mass index. The nitro counseling program was performed over three and half months. Percentage, mean value, standard deviation, Chi-square (X2), T paired test, and proportion probability ( p-value), when p < 0.05 or < 0.02 it is statistically significant difference.

Results

The results revealed that 34.1% of students weren’t aware of obesity pre-counseling, while it was improved to 93.1% post-nitro counseling implementation with highly statistical significance ( p < 0.001). Less than half of adolescence were obesity class1, and more than one-third were obesity class II. There was a highly significant difference between students’ knowledge and their practices pre- and post-nitro counseling program implementation.

Conclusion

This study confirmed that the total effect of obesity on adolescence self-esteem, social distress, and physical health demonstrated a highly significant difference between pre- and post-implementation of nitro counseling program. In addition to, improved adolescence knowledge and practices pre- and post-counseling implementation.
New energy vehicles and the political geoecology of China’s Ecological Civilisation
Authors: Ampuero-Ruiz Pablo I
Published: February 2024

Ecological Civilisation has been China’s policy concept to frame its environmental sustainability strategy. While it lacks a clear definition, its multiple practices show a reliance on the development and implementation of technologies that reduce pollution levels and beautify the country. One of those technologies are New Energy Vehicles (NEV), which stand at the centre of an e-mobility transition across urban China. I engage with the growing debate on China’s Ecological Civilisation and e-mobility transition by reflecting on the critical junctions between this policy concept and the NEV industry. I suggest that the New Energy requirements of China’s Ecological Civilisation rely on power relations that enhance state capacity domestically and transnationally. In this sense, the pursuit of New Energy to build China’s Ecological Civilisation relies on moral and sovereign forms of power. In a context of increasing tensions between China and countries in the North Atlantic, my contribution shows that these forms of power enhance China’s state capacity through its economic infrastructure, re-producing old dependencies and inequalities.
Reproducibility of Published Meta-Analyses on Clinical-Psychological Interventions
Authors: López-Nicolás Rubén , Lakens Daniel , López-López Jose A. , Rubio-Aparicio Maria , Sandoval-Lentisco Alejandro , López-Ibáñez Carmen , Blázquez-Rincón Desirée , Sánchez-Meca Julio
Published: February 2024

Meta-analysis is one of the most useful research approaches, the relevance of which relies on its credibility. Reproducibility of scientific results could be considered as the minimal threshold of this credibility. We assessed the reproducibility of a sample of meta-analyses published between 2000 and 2020. From a random sample of 100 articles reporting results of meta-analyses of interventions in clinical psychology, 217 meta-analyses were selected. We first tried to retrieve the original data by recovering a data file, recoding the data from document files, or requesting it from original authors. Second, through a multistage workflow, we tried to reproduce the main results of each meta-analysis. The original data were retrieved for 67% (146/217) of meta-analyses. Although this rate showed an improvement over the years, in only 5% of these cases was it possible to retrieve a data file ready for reuse. Of these 146, 52 showed a discrepancy larger than 5% in the main results in the first stage. For 10 meta-analyses, this discrepancy was solved after fixing a coding error of our data-retrieval process, and for 15 of them, it was considered approximately reproduced in a qualitative assessment. In the remaining meta-analyses (18%, 27/146), different issues were identified in an in-depth review, such as reporting inconsistencies, lack of data, or transcription errors. Nevertheless, the numerical discrepancies were mostly minor and had little or no impact on the conclusions. Overall, one of the biggest threats to the reproducibility of meta-analysis is related to data availability and current data-sharing practices in meta-analysis.
Non-heroic DIY in the (new) no future: Reflections from the Basque Country
Authors: del Amo Castro Ion Andoni , Smith Nicholas , Rodríguez Arkaute Natxo
Published: February 2024

The present article explores the changes and continuities in the Do It Yourself (DIY) ethos and practices in the Basque Country from the 1960s to the 2020s. In this context, music has had an outsized importance, linked especially to social and cultural movements. We base our analysis in previous works, cultural analysis, and in debates and round tables that we have organised with several young musical bands. The four decades from 1960s are characterised, therefore, by a heroic DIY ethos and practices, inspired by counterculture and nationalism. In the present day, we can talk about a different line on DIY, which paints it as non-heroic, something less idealistic and more conformist/conventional usage of DIY, driven by the new no-future ethos of trap music and online culture.
American indie cinema between DIY cultures and global Hollywood: Exploring the paradigm of Slacker
Authors: Psaltidis Nico
Published: February 2024

A landmark of the 1990s independent cinema and emblem of Generation X, Slacker (1991) is a significant event in the genealogy of Austin, Texas DIY scenes, with a long-lasting impact on the city's film industry and cultural history. The article applies research on DIY to the realm of American independent cinema, using Richard Linklater's iconic film to define indie cinema as a complex idea, linking the global media industries to cult performers emerging from intense subcultural contexts. After touching upon the history of American independent cinema, the film's content is read against its production networks, suggesting the historical role of DIY media ecologies in the value chain of Hollywood. The flexible career pathways emanating from Slacker chart various fates that creative labour undergoes in today's media industries. This methodological perspective reinforces a relational approach to the study of independent cinema, suggesting possible lines of inquiry for future research.
Management of acute breakthrough hemolysis with intensive pegcetacoplan dosing in patients with PNH
Authors: Griffin Morag , Kelly Richard J , Panse Jens , de Castro Carlos , Szer Jeff , Horneff Regina , Tan Lisa , Yeh Michael , Peffault de Latour Régis
Published: February 2024

Paroxysmal nocturnal hemoglobinuria (PNH) is characterized by complement-mediated intravascular hemolysis leading to anemia, fatigue, and potentially life-threatening thrombotic complications. Breakthrough hemolysis (BTH) was first described in patients with PNH treated with terminal complement C5 inhibitors when intravascular hemolysis reoccurred despite treatment. Pegcetacoplan, the first proximal complement C3 inhibitor, offers broad hemolysis control in patients with PNH. While experience of managing BTH on C5 inhibitors is documented, very limited guidance exists for proximal complement inhibitors. This interim analysis assessed the effect of intensive treatment with pegcetacoplan following an acute BTH event in a subset of patients enrolled in the ongoing open-label extension study of pegcetacoplan in PNH. Thirteen patients with acute BTH included in the analysis received either a single IV dose of 1080 mg (n = 4) or 1080 mg subcutaneous (SC) dosing on 3 consecutive days (n = 9). A potential, clinically-relevant complement-amplifying condition, such as infection or vaccination, was reported in approximately half of the patients experiencing an acute BTH. Lactate dehydrogenase (LDH) levels decreased between day 1 and day 2 in 8 of 12 evaluable patients and in all 13 patients at day 7 to 12. Nine of 13 patients (69%) achieved LDH <2× the upper limit of normal by day 14 to 19. All adverse events associated with the acute BTH event were considered resolved by the investigators. Overall, intensive treatment with pegcetacoplan was safe and well tolerated. These novel data support effective management of acute BTH events in patients on pegcetacoplan with intensive IV or SC pegcetacoplan dosing. This trial was registered at www.clinicaltrials.gov as #NCT03531255.
Deciphering the regulatory landscape of murine splenic response to anemic stress at single-cell resolution
Authors: Yang Chong , Yokomori Rui , Chua Lee Hui , Tan Shi Hao , Koh Mun Yee , Totani Haruhito , Sanda Takaomi , Suda Toshio
Published: February 2024

Stress erythropoiesis can be influenced by multiple mediators through both intrinsic and extrinsic mechanisms in early erythroid precursors. Single-cell RNA sequencing was conducted on spleen tissue isolated from mice subjected to phenylhydrazine and serial bleeding to explore novel molecular mechanisms of stress erythropoiesis. Our results showed prominent emergence of early erythroblast populations under both modes of anemic stress. Analysis of gene expression revealed distinct phases during the development of emerging erythroid cells. Interestingly, we observed the presence of a "hiatus" subpopulation characterized by relatively low level of transcriptional activities that transitions between early stages of emerging erythroid cells, with moderate protein synthesis activities. Moreover, single-cell analysis conducted on macrophage populations revealed distinct transcriptional programs in Vcam1+ macrophages under stress. Notably, a novel marker, CD81, was identified for labeling central macrophages in erythroblastic islands (EBIs), which is functionally required for EBIs to combat anemic stress. These findings offer fresh insights into the intrinsic and extrinsic pathways of early erythroblasts' response to stress, potentially informing the development of innovative therapeutic approaches for addressing anemic-related conditions.
Platelet-derived growth factor signaling in pericytes promotes hypothalamic inflammation and obesity
Authors: Okekawa Akira , Wada Tsutomu , Onogi Yasuhiro , Takeda Yuki , Miyazawa Yuichiro , Sasahara Masakiyo , Tsuneki Hiroshi , Sasaoka Toshiyasu
Published: February 2024

Background

Pericytes are a vital component of the blood–brain barrier, and their involvement in acute inflammation was recently suggested. However, it remains unclear whether pericytes contribute to hypothalamic chronic inflammation and energy metabolism in obesity. The present study investigated the impact of pericytes on the pathophysiology of obesity by focusing on platelet-derived growth factor (PDGF) signaling, which regulates pericyte functions.

Methods

Tamoxifen-inducible systemic conditional PDGF receptor β knockout mice (Pdgfrb∆SYS-KO) and Calcium/calmodulin-dependent protein kinase type IIa (CaMKIIa)-positive neuron-specific PDGF receptor β knockout mice (Pdgfrb∆CaMKII-KO) were fed a high-fat diet, and metabolic phenotypes before and 3 to 4 weeks after dietary loading were examined. Intracellular energy metabolism and relevant signal transduction in lipopolysaccharide- and/or platelet-derived growth factor-BB (PDGF-BB)-stimulated human brain pericytes (HBPCs) were assessed by the Seahorse XFe24 Analyzer and Western blotting. The pericyte secretome in conditioned medium from HBPCs was studied using cytokine array kit, and its impact on polarization was examined in bone marrow-derived macrophages (BMDMs), which are microglia-like cells.

Results

Energy consumption increased and body weight gain decreased after high-fat diet loading in Pdgfrb∆SYS-KO mice. Cellular oncogene fos (cFos) expression increased in proopiomelanocortin (POMC) neurons, whereas microglial numbers and inflammatory gene expression decreased in the hypothalamus of Pdgfrb∆SYS-KO mice. No significant changes were observed in Pdgfrb∆CaMKII-KO mice. In HBPCs, a co-stimulation with lipopolysaccharide and PDGF-BB shifted intracellular metabolism towards glycolysis, activated mitogen-activated protein kinase (MAPK), and modulated the secretome to the inflammatory phenotype. Consequently, the secretome showed an increase in various proinflammatory chemokines and growth factors including Epithelial-derived neutrophil-activating peptide 78 (C-X-C motif chemokine ligand (CXCL)5), Thymus and activation-regulated chemokine (C–C motif chemokine (CCL)17), Monocyte chemoattractant protein 1 (CCL2), and Growth-regulated oncogene α (CXCL1). Furthermore, conditioned medium from HBPCs stimulated the inflammatory priming of BMDMs, and this change was abolished by the C-X-C motif chemokine receptor (CXCR) inhibitor. Consistently, mRNA expression of CXCL5 was elevated by lipopolysaccharide and PDGF-BB treatment in HBPCs, and the expression was significantly lower in the hypothalamus of Pdgfrb∆SYS-KO mice than in control Pdgfrbflox/flox mice (FL) following 4 weeks of HFD feeding.

Conclusions

PDGF receptor β signaling in hypothalamic pericytes promotes polarization of macrophages by changing their secretome and contributes to the progression of obesity.
Validity and reliability of Arabic version of pediatric migraine disability assessment scale (Child Self-Report versus Parent Proxy-Report): a multi-center study
Authors: Magdy Rehab , Hassan Amr , Mohammed Zeinab , Abdeltwab Mohamed A. , Ghaffar Nawal F. Abdel , Hussein Mona
Published: February 2024

Background

Pediatric Migraine Disability Assessment (PedMIDAS) is one of the most frequently used questionnaires to assess disability from migraine in pediatric patients. This work aimed to evaluate the validity and test–retest reliability of the Arabic version of the child self-report versus the parent proxy report PedMIDAS. We also aimed to test the agreement between children's and parents' reports of the scale.

Methods

PedMIDAS was subjected to translation and back-translation, then applied to 112 pediatric patients fulfilling the migraine diagnostic criteria. This cross-sectional study was conducted on two visits, one week apart. At visit 1, the following data were obtained from the included pediatric patients: disease duration, migraine type, current treatment regimen, monthly migraine days (MMD) during the last month preceding the enrollment, and migraine intensity using the visual analogue scale. Then, each child and his parent were independently asked to fill out PedMIDAS and Child Self-Report of the Pediatric Quality of Life Inventory™ 4.0 (PedsQL™) to test the convergent validity of PedMIDAS. At visit 2, each child was requested to complete PedMIDAS again, and so was the parent to evaluate test–retest reliability.

Results

Cronbach’s alpha was estimated to be 0.94 for each instrument. For the child-self report PedMIDAS, the average measure intraclass correlation coefficient (ICC) value was 0.992 (95%CI = 0.989–0.995), while it was estimated to be 0.990 for the parent-proxy report with 95%CI = 0.985–0.993, indicating excellent test–retest reliability for both instruments. The child-self report and the parent-proxy report PedMIDAS scores were significantly correlated with MMD, VAS, and all domains of the corresponding PedsQL, supporting convergent validity for both instruments. Agreement between parent and child on disability grading categories of PedMIDAS was substantial (κ = 0.644).

Conclusion

The Arabic version of PedMIDAS was a valid and reliable instrument to assess disability from migraine in Arabic-speaking pediatric patients with migraine. Parent reports can be valuable as a complement to child reports for a comprehensive assessment of migraine.
Arterial hypertension in the chronic evolution of migraine: bystander or risk factor? An overview
Authors: Mazzacane Federico , Vaghi Gloria , Cotta Ramusino Matteo , Perini Giulia , Costa Alfredo
Published: February 2024

Background

Several risk factors are associated with the chronic evolution of migraine. Clinical and preclinical studies have provided data about the role of hypertension (HT) as one of the potential modifiable risk factors of chronic migraine (CM). This review is focused on the biological and clinical evidence supporting common mechanisms underlying HT and migraine and the potential role of HT in the transition from episodic to chronic migraine.

Methods

We conducted a narrative review from a literature search covering the available evidence from studies investigating: i) the role of HT in the transition to CM in clinical practice; ii) the biological mechanisms potentially underpinning the association between HT and evolution to CM; iii) the role of antihypertensive medications in migraine prophylaxis.

Results

HT proved to be at the base of multiple mechanisms underlying migraine and migraine chronicization. Endothelial dysfunction, blood–brain barrier alterations, calcitonin gene-related peptide signaling, and renin–angiotensin–aldosterone system dysregulation are involved in the worsening effect of HT on migraine frequency, and the role of HT in the transition to CM is supported by clinical observations.

Conclusions

The observed evidence supports HT contribution to CM evolution due to shared pathophysiologic mechanisms. While a bidirectional influence appears to be ascertained, data are still lacking about the one-way role of HT as direct risk factor for CM transition. Further research is needed to confirm a causal role of HT in this process.
Dynamic changes in glymphatic function in reversible cerebral vasoconstriction syndrome
Authors: Wu Chia-Hung , Kuo Yu , Ling Yu-Hsiang , Wang Yen-Feng , Fuh Jong-Ling , Lirng Jiing-Feng , Wu Hsiu-Mei , Wang Shuu-Jiun , Chen Shih-Pin
Published: February 2024

Background

The pathophysiology of the reversible cerebral vasoconstriction syndrome (RCVS) remains enigmatic and the role of glymphatics in RCVS pathophysiology has not been evaluated. We aimed to investigate RCVS glymphatic dynamics and its clinical correlates.

Methods

We prospectively evaluated the glymphatic function in RCVS patients, with RCVS subjects and healthy controls (HCs) recruited between August 2020 and November 2023, by calculating diffusion-tensor imaging along the perivascular space (DTI-ALPS) index under a 3-T MRI. Clinical and vascular (transcranial color-coded duplex sonography) investigations were conducted in RCVS subjects. RCVS participants were separated into acute (≤ 30 days) and remission (≥ 90 days) groups by disease onset to MRI interval. The time-trend, acute stage and longitudinal analyses of the DTI-ALPS index were conducted. Correlations between DTI-ALPS index and vascular and clinical parameters were performed. Bonferroni correction was applied to vascular investigations (q = 0.05/11).

Results

A total of 138 RCVS patients (mean age, 46.8 years ± 11.8; 128 women) and 42 HCs (mean age, 46.0 years ± 4.5; 35 women) were evaluated. Acute RCVS demonstrated lower DTI-ALPS index than HCs (p < 0.001) and remission RCVS (p < 0.001). A continuously increasing DTI-ALPS trend after disease onset was demonstrated. The DTI-ALPS was lower when the internal carotid arteries resistance index and six-item Headache Impact test scores were higher. In contrast, during 50–100 days after disease onset, the DTI-ALPS index was higher when the middle cerebral artery flow velocity was higher.

Conclusions

Glymphatic function in patients with RCVS exhibited a unique dynamic evolution that was temporally coupled to different vascular indices and headache-related disabilities along the disease course. These findings may provide novel insights into the complex interactions between glymphatic transport, vasomotor control and pain modulation.
Dual-targeted nano-encapsulation of neonatal porcine islet-like cell clusters with triiodothyronine-loaded bifunctional polymersomes
Authors: Lee Sang Hoon , Kim Minse , Lee Eun-Jin , Ahn Sun Mi , Ahn Yu-Rim , Choi Jaewon , Kang Jung-Taek , Kim Hyun-Ouk
Published: February 2024

There is growing evidence that neonatal porcine islet-like cell clusters (NPCCs) isolated from piglets can be used to treat type 1 diabetes in humans. However, graft rejection is a common complication in humans owing to the prevalence of xenoantigens in porcine. Therefore, researchers have investigated various islet encapsulation techniques that could protect against these antigens. To this end, this study presents a robust nano-encapsulation method based on bifunctional polymersomes (PSomes), in which N-hydroxysuccinimide (NHS) and maleimide (Mal) groups conjugated to the PSomes terminal interact with the amine and thiol groups on the surface of NPCCs to induce dual targeting via two covalent bonds. The findings indicate that the ratio of NHS to Mal on PSomes is optimal for dual targeting. Moreover, triiodothyronine (T3) is known to promotes pancreatic islet maturation and differentiation of endocrine cells into beta cells. T3 encapsulated in PSomes is shown to increase the glucose sensitivity of NPCCs and enhance insulin secretion from NPCCs. Furthermore, improvements in the nano-encapsulation efficiency and insulin-secreting capability of NPCCs through dual targeting via dual-Psomes are demonstrated. In conclusion, the proposed nano-encapsulation technique could pave the way for significant advances in islet nano-encapsulation and the imprevement of NPCC immaturity via T3 release.
Personnel adjustments during the Covid-19 pandemic: did co-determination make a difference?
Authors: Fackler Daniel , Schnabel Claus , Stegmaier Jens
Published: February 2024

Using a unique dataset of establishments in Germany surveyed during the Covid-19 pandemic, this study investigates whether personnel adjustments during the crisis differed between establishments with and without a works council. Our regression analyses show that the hiring and dismissal rate as well as the churning rate were lower in establishments with a works council. In contrast, the net employment growth rate over the pandemic and the implementation of short-time work did not differ significantly between establishments with and without a works council. We conclude that worker co-determination did indeed make a difference in terms of higher employment stability for the incumbent workforce during the pandemic.
Modulation of long-term potentiation following microdoses of LSD captured by thalamo-cortical modelling in a randomised, controlled trial
Authors: Murphy Robin J. , Godfrey Kate , Shaw Alexander D. , Muthukumaraswamy Suresh , Sumner Rachael L.
Published: February 2024

Background

Microdosing psychedelics is a phenomenon with claimed cognitive benefits that are relatively untested clinically. Pre-clinically, psychedelics have demonstrated enhancing effects on neuroplasticity, which cannot be measured directly in humans, but may be indexed by non-invasive electroencephalography (EEG) paradigms. This study used a visual long-term potentiation (LTP) EEG paradigm to test the effects of microdosed lysergic acid diethylamide (LSD) on neural plasticity, both acutely while on the drug and cumulatively after microdosing every third day for six weeks. Healthy adult males (n = 80) completed the visual LTP paradigm at baseline, 2.5 h following a dose of 10 µg of LSD or inactive placebo, and 6 weeks later after taking 14 repeated microdoses. Visually induced LTP was used as indirect index of neural plasticity. Surface level event-related potential (ERPs) based analyses are presented alongside dynamic causal modelling of the source localised data using a generative thalamocortical model (TCM) of visual cortex to elucidate underlying synaptic circuitry.

Results

Event-related potential (ERP) analyses of N1b and P2 components did not show evidence of changes in visually induced LTP by LSD either acutely or after 6 weeks of regular dosing. However modelling the complete timecourse of the ERP with the TCM demonstrated changes in laminar connectivity in primary visual cortex. This primarily included changes to self-gain and inhibitory input parameters acutely. Layer 2/3 to layer 5 excitatory connectivity was also different between LSD and placebo groups. After regular dosing only excitatory input from layer 2/3 into layer 5 and inhibitory input into layer 4 were different between groups.

Conclusions

Without modulation of the ERPs it is difficult to relate the findings to other studies visually inducing LTP. It also indicates the classic peak analysis may not be sensitive enough to demonstrate evidence for changes in LTP plasticity in humans at such low doses. The TCM provides a more sensitive approach to assessing changes to plasticity as differences in plasticity mediated laminar connectivity were found between the LSD and placebo groups.

Trial registration: ANZCTR registration number ACTRN12621000436875; Registered 16/04/2021 https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=381476.
Risk factors for gastroesophageal reflux disease: a population-based study
Authors: Sadafi Sepehr , Azizi Ali , Pasdar Yahya , Shakiba Ebrahim , Darbandi Mitra
Published: February 2024

Background

Gastroesophageal reflux disease (GERD) in the long term reduces the quality of life, leading to digestive diseases. The present study aims to determine the risk factors for GERD.

Method

This study was conducted on 9,631 adults aged 35–65 years. The demographic characteristics, behavioral habits, nutritional intake, physical activity, anthropometric indices, and GERD data were extracted from the databank related to the Ravansar non-communicable diseases (RaNCD). Statistical analysis was performed using logistic regression models.

Results

The prevalence of GERD was 10.99% (n = 1,058). The GERD was higher among older age and women. After adjusting for age and sex, the odds of GERD among current smokers was 23% higher than non-smokers. Drinking increased odds of GERD (OR: 1.51; 95% CI: 1.13, 1.99). The odds of GERD among depressed individuals were 46% higher than non-depressed. In addition, a significant relationship was observed between the high intake of sweets and desserts with increased GERD (OR: 1.02, 95% CI: 1.01, 1.03). Further, high intake of fiber (OR: 0.98, 95% CI: 0.97, 0.99) and dairy (OR: 0.99, 95% CI: 0.98, 0.99) was related to reducing the odds of GERD. Furthermore, a significant relationship was reported between the waist hip ratio (WHR) and visceral fat area (VFA) with increased odds of GERD. Finally, the physical activity level was inversely related to GERD.

Conclusion

Based on the results, smoking, alcohol, inactivity, high intake of sweets and desserts, low intake of fiber, depression, visceral fat, and obesity are considered as risk factors for GERD. Modifying lifestyle and behavioral habits prevent GERD.
The use of a multi-disciplinary geriatric telemedicine service (TELEG) and its acceptance at a tertiary care centre in Malaysia
Authors: Ting Chuo Yew , Abdul Halim Nur Hidayati , Ling Jia Nee , Tiong Ing Khieng , Ahmad Shauki Nor Izzah H. J. , Lee Yew Fong , Osman Nor Anizah , Chai Gin Wei , Ung Shin Han , Ang Melinda
Published: February 2024

Background

The COVID-19 pandemic has fueled the widespread adoption of telemedicine in healthcare, particularly in Sarawak, Malaysia. This study investigates the use and acceptance of Sarawak’s inaugural multidisciplinary geriatric telemedicine service, TELEG.

Methods

This cross-sectional study took place at the Sarawak Heart Centre’s geriatric department from July 1, 2021, to April 30, 2022. Convenient sampling included all TELEG-enrolled patients during this period, to achieve minimum sample size of 148. TELEG’s utilization was assessed in terms of medication therapy and treatment plan optimization, as well as enhanced healthcare accessibility. Participants’ acceptance of TELEG was measured using the Service User Technology Acceptability Questionnaire (SUTAQ) administered through Google Forms. Descriptive statistics percentages illustrated the proportion of participants who found TELEG moderately to highly acceptable. Associations between baseline characteristics and overall acceptance were explored through bivariate analyses, including Pearson’s correlation test, independent t-test, and ANOVA. The influence of six SUTAQ dimensions on overall acceptance, multivariable linear regression using enter method was employed. Statistical significance was determined by p-values less than 0.5.

Results

Among 180 geriatric patients enrolled in TELEG during the study period, 149 agreed to participate. TELEG led to medication therapy optimization for 88.6% of participants, primarily involving dose adjustment (44.7%), de-prescribing (31.8%), and prescribing (15.9%). Additionally, 53.8% received treatment plan optimization, predominantly in the form of self-care education (56.3%), referrals for further treatment (33.8%), additional laboratory investigations (29.6%), and increased monitoring (26.8%). Among those educated in self-care (n = 40), dietary intake (27.5%), lower limb exercise (25.0%), and COVID-19 vaccination (12.5%) were the most common topics. All participants expressed moderate to high acceptance of TELEG (mean = 4.9, SD = 0.65, on a scale of 1 to 6). Notably, care personnel concern (B = 0.256;p < 0.001) had the most significant impact on overall acceptance.

Conclusion

This pioneering study evaluates the utilization and user acceptance of a geriatric telemedicine service in the region, providing valuable insights to support its expansion. Follow-up surveys or interviews to gain insights into users’ experiences are crucial to further enhance acceptance.
Prevalence of celiac-related antibodies and its impact on metabolic control in Egyptian children with type 1 diabetes mellitus
Authors: Baseer Khaled A. Abdel , Mohammed Abdallah E. , Elwafa Aya Mohamed Abo , Sakhr Hala M.
Published: February 2024

Objective

The simultaneous presence of celiac disease and type 1 diabetes (T1DM) is coupled with more hazards of comorbidities and complications. This current study aimed to screen for celiac disease in Egyptian children with type 1 diabetes and evaluate its impact on glycemic control.

Methods

A cross-sectional study was verified with 200 Egyptian children diagnosed with T1DM and having a diabetic duration of less than five years. Testing for anti-tissue transglutaminase IgA (tTG-IgA), anti-tissue transglutaminase IgG (tTG-IgG), anti-Endomysial IgA (EMA), and Hb A1c levels were done.

Results

The serological screening revealed that 11 cases (5.5%) tested positive; 8 children with T1DM (4.0%) showed tTG-IgA antibodies ≥ 10 times the upper limit of normal (ULN) with at least one symptom; and 3 cases (1.5%) had levels between 20 and 50 IU/ml (considering a cut-off point of 10 U/ML for positive results). Intestinal biopsy was performed for these three cases, with one case detected to have subtotal villous atrophy, resulting in an overall prevalence of celiac disease in T1DM as 4.5%. Children with positive screening exhibited a higher insulin dose, a higher HbA1c, an increased frequency of hypoglycemic attacks, and recurrent DKA compared to negative cases. A negative correlation was detected between tTG-IgA antibodies with height Z score and hemoglobin level, while a positive correlation was found between tTG-IgA antibodies and HbA1c level.

Conclusion

Undiagnosed celiac disease in children with T1DM negatively impacted metabolic control and affected their general health.