bluuebunny/crossref_metadata_2025 huggingface dataset:
Breaking down barriers to COPD management in primary care: applying the updated 2023 Canadian Thoracic Society guideline for pharmacotherapy
Authors: Kaplan Alan , Babineau Amanda , Hauptman Robert , Levitz Suzanne , Lin Peter , Yang Molly
Published: August 2024

Chronic obstructive pulmonary disease (COPD) is a highly prevalent yet under-recognized and sub-optimally managed disease that is associated with substantial morbidity and mortality. Primary care providers (PCPs) are at the frontlines of COPD management, and they play a critical role across the full spectrum of the COPD patient journey from initial recognition and diagnosis to treatment optimization and referral to specialty care. The Canadian Thoracic Society (CTS) recently updated their guideline on pharmacotherapy in patients with stable COPD, and there are several key changes that have a direct impact on COPD management in the primary care setting. Notably, it is the first guideline to formally make recommendations on mortality reduction in COPD, which elevates this disease to the same league as other chronic diseases that are commonly managed in primary care and where optimized pharmacotherapy can reduce all-cause mortality. It also recommends earlier and more aggressive initial maintenance inhaler therapy across all severities of COPD, and preferentially favors the use of single inhaler therapies over multiple inhaler regimens. This review summarizes some of the key guideline changes and offers practical tips on how to implement the new recommendations in primary care. It also addresses other barriers to optimal COPD management in the primary care setting that are not addressed by the guideline update and suggests strategies on how they could be overcome.
The microbiota: a crucial mediator in gut homeostasis and colonization resistance
Authors: Chen Yiding , Xiao Ling , Zhou Min , Zhang Hu
Published: August 2024

The gut microbiota is a complex and diverse community of microorganisms that colonizes the human gastrointestinal tract and influences various aspects of human health. These microbes are closely related to enteric infections. As a foreign entity for the host, commensal microbiota is restricted and regulated by the barrier and immune system in the gut and contributes to gut homeostasis. Commensals also effectively resist the colonization of pathogens and the overgrowth of indigenous pathobionts by utilizing a variety of mechanisms, while pathogens have developed strategies to subvert colonization resistance. Dysbiosis of the microbial community can lead to enteric infections. The microbiota acts as a pivotal mediator in establishing a harmonious mutualistic symbiosis with the host and shielding the host against pathogens. This review aims to provide a comprehensive overview of the mechanisms underlying host-microbiome and microbiome-pathogen interactions, highlighting the multi-faceted roles of the gut microbiota in preventing enteric infections. We also discuss the applications of manipulating the microbiota to treat infectious diseases in the gut.
Construction of an amylolytic Saccharomyces cerevisiae strain with high copies of α-amylase and glucoamylase genes integration for bioethanol production from sweet potato residue
Authors: Wang Xin , Guo Na , Hu Jingting , Gou Chenchen , Xie Xinyue , Zheng Haobo , Liao Aimei , Huang Jihong , Hui Ming , Liu Na
Published: August 2024

Sweet potato residue (SPR) is the by-product of starch extraction from fresh sweet potatoes and is rich in carbohydrates, making it a suitable substrate for bioethanol production. An amylolytic industrial yeast strain with co-expressing α-amylase and glucoamylase genes would combine enzyme production, SPR hydrolysis, and glucose fermentation into a one-step process. This consolidated bioprocessing (CBP) shows great application potential in the economic production of bioethanol. In this study, a convenient heterologous gene integration method was developed. Eight copies of a Talaromyces emersonii α-amylase expression cassette and eight copies of a Saccharomycopsis fibuligera glucoamylase expression cassette were integrated into the genome of industrial diploid Saccharomyces cerevisiae strain 1974. The resulting recombinant strains exhibited clear transparent zones in the iodine starch plates, and SDS-PAGE analysis indicated that α-amylase and glucoamylase were secreted into the culture medium. Enzymatic activity analysis demonstrated that the optimal temperature for α-amylase and glucoamylase was 60–70°C, and the pH optima for α-amylase and glucoamylase was 4.0 and 5.0, respectively. Initially, soluble corn starch with a concentration of 100 g/L was initially used to evaluate the ethanol production capability of recombinant amylolytic S. cerevisiae strains. After 7 days of CBP fermentation, the α-amylase-expressing strain 1974-temA and the glucoamylase-expressing strain 1974-GA produced 33.03 and 28.37 g/L ethanol, respectively. However, the 1974-GA-temA strain, which expressed α-amylase and glucoamylase, produced 42.22 g/L ethanol, corresponding to 70.59% of the theoretical yield. Subsequently, fermentation was conducted using the amylolytic strain 1974-GA-temA without the addition of exogenous α-amylase and glucoamylase, which resulted in the production of 32.15 g/L ethanol with an ethanol yield of 0.30 g/g. The addition of 20% glucoamylase (60 U/g SPR) increased ethanol concentration to 50.55 g/L, corresponding to a theoretical yield of 93.23%, which was comparable to the ethanol production observed with the addition of 100% α-amylase and glucoamylase. The recombinant amylolytic strains constructed in this study will facilitate the advancement of CBP fermentation of SPR for the production of bioethanol.
The clinical significance of endoplasmic reticulum stress related genes in non-small cell lung cancer and analysis of single nucleotide polymorphism for CAV1
Authors: Li Shuang , Chen Junting , Zhou Baosen
Published: August 2024

In recent years, protein homeostasis imbalance caused by endoplasmic reticulum stress has become a major hallmark of cancer. Studies have shown that endoplasmic reticulum stress is closely related to the occurrence, development, and drug resistance of non-small cell lung cancer, however, the role of various endoplasmic reticulum stress-related genes in non-small cell lung cancer is still unclear. In this study, we established an endoplasmic reticulum stress scores based on the Cancer Genome Atlas for non-small cell lung cancer to reflect patient features and predict prognosis. Survival analysis showed significant differences in overall survival among non-small cell lung cancer patients with different endoplasmic reticulum stress scores. In addition, endoplasmic reticulum stress scores was significantly correlated with the clinical features of non-small cell lung cancer patients, and can be served as an independent prognostic indicator. A nomogram based on endoplasmic reticulum stress scores indicated a certain clinical net benefit, while ssGSEA analysis demonstrated that there was a certain immunosuppressive microenvironment in high endoplasmic reticulum stress scores. Gene Set Enrichment Analysis showed that scores was associated with cancer pathways and metabolism. Finally, weighted gene co-expression network analysis displayed that CAV1 was closely related to the occurrence of non-small cell lung cancer. Therefore, in order to further analyze the role of this gene, Chinese non-smoking females were selected as the research subjects to investigate the relationship between CAV1 rs3779514 and susceptibility and prognosis of non-small cell lung cancer. The results showed that the mutation of rs3779514 significantly reduced the risk of non-small cell lung cancer in Chinese non-smoking females, but no prognostic effect was found. In summary, we proposed an endoplasmic reticulum stress scores, which was an independent prognostic factor and indicated immune characteristics in the microenvironment of non-small cell lung cancer. We also validated the relationship between single nucleotide polymorphism locus of core genes and susceptibility to non-small cell lung cancer.
Coordination between midcingulate cortex and retrosplenial cortex in pain regulation
Authors: Qiu Yunya , Lian Yan-Na , Wu Cheng , Liu Li , Zhang Chen , Li Xiang-Yao
Published: August 2024

Introduction

The cingulate cortex, with its subregions ACC, MCC, and RSC, is key in pain processing. However, the detailed interactions among these regions in modulating pain sensation have remained unclear.

Methods

In this study, chemogenetic tools were employed to selectively activate or inhibit neuronal activity in the MCC and RSC of rodents to elucidate their roles in pain regulation.Results: Our results showed that chemogenetic activation in both the RSC and MCC heightened pain sensitivity. Suppression of MCC activity disrupted the RSC’s regulation of both mechanical and thermal pain, while RSC inhibition specifically affected the MCC’s regulation of thermal pain.

Discussion

The findings indicate a complex interplay between the MCC and RSC, with the MCC potentially governing the RSC’s pain regulatory mechanisms. The RSC, in turn, is crucial for the MCC’s control over thermal sensation, revealing a collaborative mechanism in pain processing.

Conclusion

This study provides evidence for the MCC and RSC’s collaborative roles in pain regulation, highlighting the importance of their interactions for thermal and mechanical pain sensitivity. Understanding these mechanisms could aid in developing targeted therapies for pain disorders.
Induction of seizures and initiation of epileptogenesis by pilocarpine in zebrafish larvae
Authors: Gawel Kinga , Hulas-Stasiak Monika , Marszalek-Grabska Marta , Grenda Anna , Siekierska Aleksandra , Kosheva Nataliia , van der Ent Wietske , Esguerra Camila V. , Krawczyk Pawel , Turski Waldemar A.
Published: August 2024

Objective

Preclinical models of seizures and epilepsy in rodents contributed substantially to the discovery of currently available antiseizure medications. These were also broadly used for investigation of processes of epileptogenesis. Nevertheless, rodent models pose some limitations, thus, new models using alternative species are in high demand. The aim of this study was to describe a new model of seizures/epilepsy induced by the cholinomimetic agent, pilocarpine (PILO), in larval zebrafish.

Methods

Local field potential (LFP) recordings were conducted to analyze electroencephalographic discharges and correlate it with larval behavior. Hematoxylin and eosin (H&E) staining, as well as TUNEL staining were performed to analyze morphology and apoptosis, respectively. Real-time quantitative polymerase chain reaction (qRT-PCR) was undertaken for gene expression analysis.

Results

Acute exposure to PILO, in a concentration-dependent manner, induces electroencephalographic discharges in larval zebrafish, which behaviorally manifest as decreased locomotion and moving time, but enhanced movement velocity. The PILO-induced seizure-like activity is behaviorally distinct from this induced by the application of chemoconvulsant pentylenetetrazole (PTZ). Zebrafish larvae previously exposed to PILO (2 h), after a washing out period, exhibit spontaneous, unprovoked discharges and apoptotic changes in their brains.

Significance

Here, we comprehensively investigated a new model of PILO-induced seizures/epilepsy in larval zebrafish. We propose that this model may be used to study epileptogenesis and for antiseizure drug screening purposes.
Antioxidant activities, dietary nutrients, and yield potential of bitter gourd (Momordica charantia L.) lines in diverse growing environments
Authors: Jat Gograj Singh , Behera Tusar Kanti , Singh Awani Kumar , Bana Ram Swaroop , Singh Deepak , Godara Samarth , Reddy Umesh K. , Rao P. Gangadhara , Ram H. , Vinay N. D. , Kumar Sachin , Tomar Bhoopal Singh
Published: August 2024

The biotic and abiotic stresses cause a significant decline in the yield and fruit quality traits, including antioxidants and minerals, of bitter gourd when grown in open fields. Protected cultivation technology has emerged to minimize such stresses. We investigated the effect of diverse environments (hi-tech greenhouse, naturally ventilated polyhouse, insect-proof net-house, and open field) and breeding lines on earliness, yield potential, antioxidant activities, and dietary nutrients. In the GYT analysis, 12 treatment combinations involving four growing environments and three breeding lines of bitter gourd were examined. The 3-year study suggested that the cultivation of bitter gourd crops in an insect-proof net house (NH) showed superior performance in earliness, yield-attributing traits, antioxidant activities, and dietary nutrients, followed by a naturally ventilated polyhouse (NP). However, NH was on par with NP and significantly better than the open-field-grown crop. The GYT biplot analysis highlighted that the combinations of NH and Pusa Rasdar outperformed and were the most stable treatments for all the traits investigated, followed by NH in conjunction with S32 and S57 lines. This study suggests that growing bitter gourd in protected environments is the optimal strategy to achieve early market prices and improve the yield and nutritional quality of the fruits.
Efficacy of probiotics/synbiotics supplementation in patients with chronic kidney disease: a systematic review and meta-analysis of randomized controlled trials
Authors: Liu Chang , Yang Letian , Wei Wei , Fu Ping
Published: August 2024

Background

Chronic kidney disease (CKD) is a serious and steadily growing health problem worldwide. Probiotic and synbiotic supplementation are expected to improve kidney function in CKD patients by altering imbalanced intestinal flora, regulating microbiota metabolites, modulating the brain-gut axis, and reducing inflammation.

Objectives

Our aim is to report the latest and largest pooled analyses and evidence updates to explore whether probiotic and synbiotic have beneficial effects on renal function and general conditions in patients with CKD.

Methods

We conducted a systematic literature search using PubMed, Embase, Web of Science, and the Cochrane Central Register of Controlled Trials from inception until 1 December 2023. Eligible literatures were screened according to inclusion and exclusion criteria, data were extracted, and a systematic review and meta-analysis was performed. Measurements included renal function-related markers, inflammatory markers, uremic toxins, lipid metabolism-related markers and electrolytes levels.

Results

Twenty-one studies were included. The results showed that probiotic/synbiotic significantly reduced blood urea nitrogen (BUN) (standardized mean difference (SMD), −0.23, 95% confidence interval (CI) −0.41, −0.04; p = 0.02, I2 = 10%) and lowered c-reactive protein level (CRP) (SMD: −0.34; 95% CI: −0.62, −0.07; p = 0.01, I2 = 37%) in CKD patients, compared with the control group.

Conclusion

In summary, probiotic/synbiotic supplementation seems to be effective in improving renal function indices and inflammation indices in CKD patients. Subgroup analyses suggested that longer-term supplementation is more favorable for CKD patients, but there is a high degree of heterogeneity in the results of partial subgroup analyses. The efficacy of probiotic/synbiotic in treating CKD needs to be supported by more evidence from large-scale clinical studies.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42024526836, Unique identifier: CRD42024526836.
Integrating electromagnetic cancer stress with immunotherapy: a therapeutic paradigm
Authors: Fuster Mark M.
Published: August 2024

An array of published cell-based and small animal studies have demonstrated a variety of exposures of cancer cells or experimental carcinomas to electromagnetic (EM) wave platforms that are non-ionizing and non-thermal. Overall effects appear to be inhibitory, inducing cancer cell stress or death as well as inhibition in tumor growth in experimental models. A variety of physical input variables, including discrete frequencies, amplitudes, and exposure times, have been tested, but drawing methodologic rationale and mechanistic conclusions across studies is challenging. Nevertheless, outputs such as tumor cytotoxicity, apoptosis, tumor membrane electroporation and leak, and reactive oxygen species generation are intriguing. Early EM platforms in humans employ pulsed electric fields applied either externally or using interventional tumor contact to induce tumor cell electroporation with stromal, vascular, and immunologic sparing. It is also possible that direct or external exposures to non-thermal EM waves or pulsed magnetic fields may generate electromotive forces to engage with unique tumor cell properties, including tumor glycocalyx to induce carcinoma membrane disruption and stress, providing novel avenues to augment tumor antigen release, cross-presentation by tumor-resident immune cells, and anti-tumor immunity. Integration with existing checkpoint inhibitor strategies to boost immunotherapeutic effects in carcinomas may also emerge as a broadly effective strategy, but little has been considered or tested in this area. Unlike the use of chemo/radiation and/or targeted therapies in cancer, EM platforms may allow for the survival of tumor-associated immunologic cells, including naïve and sensitized anti-tumor T cells. Moreover, EM-induced cancer cell stress and apoptosis may potentiate endogenous tumor antigen-specific anti-tumor immunity. Clinical studies examining a few of these combined EM-platform approaches are in their infancy, and a greater thrust in research (including basic, clinical, and translational work) in understanding how EM platforms may integrate with immunotherapy will be critical in driving advances in cancer outcomes under this promising combination.
Efficacy and risk factors of stent placement in the treatment of malignant tracheoesophageal fistula
Authors: Wang Qingxia , Duan Zhihong , Liu Shiqi , Shi Ruihua
Published: August 2024

Background

Due to the low incidence of malignant tracheoesophageal fistula and the paucity of relevant clinical studies, the benefits of stent implantation have not been well documented. It remains unclear which factors may affect fistula closure.

Methods

Between January 2015 and January 2021, 344 patients who were diagnosed with malignant tracheoesophageal fistula at Zhongda Hospital, Southeast University, were retrospectively enrolled. Demographic and clinical data were collected. Risk factors for fistula closure identified by univariate analysis were further analyzed using multivariable logistic regression.

Results

A total of 288 patients were analyzed in this study, of which 94 were treated conservatively, 170 were treated with an esophageal stent, and 24 were treated with a tracheal stent. Among them, the delta Karnofsky’s performance status score values (after 2 weeks/before treatment [p = 0.0028], after 1 month/before treatment [p = 0.0103]) were significantly different between conservative and stent treatment. There was a significant reduction of pneumonia incidence in the stenting group (33.53%) compared to the conservative treatment group (77.05%) after one month (p <0.0001). In addition, the closure of fistulas was influenced by four independent risk factors: 1) treatment methods (p < 0.0001), 2) fistula size (p = 0.0003), 3) preoperative white blood cell count (p = 0.0042), and 4) preoperative Karnofsky’s performance status score (p = 0.0001).

Conclusions

Stent implantation has become an effective method for treating malignant tracheoesophageal fistula compared to conservative treatment. Additionally, stent implantation, smaller fistula size, lower preoperative white blood cell count, and higher preoperative Karnofsky’s performance status score suggest a better outcome.
Analysis of genome-wide association studies of low-temperature germination in Xian and Geng rice
Authors: Li Kang , Hassan Muhammad Ahmad , Guo Jinmeng , Zhao Xueyu , Gan Quan , Lin Cuixiang , Ten Bin , Zhou Kunneng , Li Min , Shi Yingyao , Ni Dahu , Song Fengshun
Published: August 2024

Rice is the leading global staple crop. Low temperatures pose negative impacts on rice’s optimal growth and development. Rice cultivars acclimating to low temperatures exhibited improved seedling emergence under direct-seeded sowing conditions, yet little is known about the genes that regulate germination at low temperatures (LTG). In this research investigation, we’ve performed whole genome sequencing for the 273 rice plant materials. Using the best linear unbiased prediction (BLUP) values for each rice material, we identified 7 LTG-related traits and performed the efficient genetic analysis and genome-wide association study (GWAS). As a result of this, 95 quantitative trait loci (QTLs) and 1001 candidate genes associated with LTG in rice were identified. Haplotype analysis and functional annotation of the candidate genes resulted in the identification of three promising candidate genes (LOC_Os08g30520 for regulating LTG4 and LTG5, LOC_Os10g02625 for regulating LTG6, LTg7 and LTG8, and LOC_Os12g31460 for regulating LTG7, LTg8 and LTG9) involving in the regulation of LTG in rice. This research provides a solid foundation for addressing the LTG issue in rice and will be valuable in future direct-seeded rice breeding programs.
Managing fruit rot diseases of Vaccinium corymbosum
Authors: Neugebauer Kerri A. , Mattupalli Chakradhar , Hu Mengjun , Oliver Jonathan E. , VanderWeide Joshua , Lu Yuzhen , Sullivan Kevin , Stockwell Virginia O. , Oudemans Peter , Miles Timothy D.
Published: August 2024

Blueberry is an important perennial fruit crop with expanding consumption and production worldwide. Consumer demand for blueberries has grown due to the desirable flavor and numerous health benefits, and fresh market production in the U.S. has risen in turn. U.S. imports have also increased to satisfy year-round consumer demand for fresh blueberries. Pre- and post-harvest fruit diseases such as anthracnose (caused by Colletotrichum spp.) and botrytis fruit rot (caused by Botrytis spp.) have a significant impact on fruit quality and consumer acceptance. These are also among the most difficult diseases to control in the blueberry cropping system. These latent pathogens can cause significant losses both in the field, and especially during transport and marketplace storage. Although both diseases result in rotted fruit, the biology and infection strategies of the causal pathogens are very different, and the management strategies differ. Innovations for management, such as improved molecular detection assays for fungicide resistance, postharvest imaging, breeding resistant cultivars, and biopesticides have been developed for improved fruit quality. Development and integration of new strategies is critical for the long-term success of the blueberry industry.
An implementation framework for evaluating the biocidal potential of essential oils in controlling Fusarium wilt in spinach: from in vitro to in planta
Authors: Mirmajlessi Mahyar , Najdabbasi Neda , Sigillo Loredana , Haesaert Geert
Published: August 2024

Fusarium wilt, caused by Fusarium oxysporum f. sp. spinaciae, causes a significant challenge on vegetative spinach and seed production. Addressing this issue necessitates continuous research focused on innovative treatments and protocols through comprehensive bioassays. Recent studies have highlighted the potential of plant-based compounds in controlling fungal diseases. The present work aims to conduct a series of experiments, encompassing both in vitro and in planta assessments, to investigate the biocontrol capabilities of different essential oils (EOs) at various application rates, with the ultimate goal of reducing the incidence of Fusarium wilt in spinach. The inhibitory effect of four plant EOs (marjoram, thyme, oregano, and tea tree) was initially assessed on the spore germination of five unknown Fusarium strains. The outcomes revealed diverse sensitivities of Fusarium strains to EOs, with thyme exhibiting the broadest inhibition, followed by oregano at the highest concentration (6.66 μL/mL) in most strains. The tested compounds displayed a diverse range of median effective dose (ED50) values (0.69 to 7.53 µL/mL), with thyme and oregano consistently showing lower ED50 values. The direct and indirect inhibitory impact of these compounds on Fusarium mycelial growth ranged from ~14% to ~100%, wherein thyme and oregano consistently exhibiting the highest effectiveness. Following the results of five distinct inoculation approaches and molecular identification, the highly pathogenic strain F-17536 (F. oxysporum f.sp. spinaciae) was chosen for Fusarium wilt assessment in spinach seedlings, employing two promising EO candidates through seed and soil treatments. Our findings indicate that colonized grain (CG) proved to be a convenient and optimal inoculation method for consistent Fusarium wilt assessment under greenhouse conditions. Seed treatments with thyme and oregano EOs consistently resulted in significantly better disease reduction rates, approximately 54% and 36% respectively, compared to soil treatments (P > 0.05). Notably, thyme, applied at 6.66 µL/mL, exhibited a favorable emergence rate (ERI), exceeding seven, in both treatments, emphasizing its potential for effective disease control in spinach seedlings without inducing phytotoxic effects. This study successfully transitions from in vitro to in planta experiments, highlighting the potential incorporation of EOs into integrated disease management for Fusarium wilt in spinach production.
Understanding users of online energy efficiency counseling: comparison to representative samples in Norway
Authors: Klöckner Christian A. , Nayum Alim , Vesely Stepan
Published: August 2024

Introduction

To achieve substantial energy efficiency improvements in the privately owned building stock, it is important to communicate with potential renovators at the right point in time and provide them with targeted information to strengthen their renovation ambitions. The European Union recommends using one-stop-shops (OSSs), which provide information and support throughout the whole process, from planning to acquisition of funding, implementation, and evaluation as a measure to remove unnecessary barriers.

Methods

For this paper, we invited visitors of two Norwegian websites with OSS characteristics to answer an online survey about their renovation plans and energy efficiency ambitions. The participants visited the websites out of their own interest; no recruitment for the websites was conducted as part of the study (N = 437). They also rated a range of psychological drivers, facilitators, and barriers to including energy upgrades in a renovation project. Their answers were then compared to existing data from representative samples of Norwegian households regarding home renovation in 2014, 2018, and 2023, as well as data from a sample of people who were engaged in renovation projects in 2014, which was collected by the research team with a similar online survey. Furthermore, 78 visitors completed a brief follow-up online survey one year later to report the implemented measures.

Results

We found that visitors of the websites are involved in more comprehensive renovation projects and have substantially higher ambitions for the upgrade of energy efficiency compared to the representative samples. They also perceive stronger personal and social norms, as well as have a different profile of facilitators and barriers.

Discussion

The findings suggest to policymakers that OSSs should be marketed especially to people motivated to upgrade energy efficiency but lack information and are unable to implement their plans alone. Also, the construction industry might refer interested people to such low-threshold online solutions to assist informed and more ambitious decisions.
Online screening for excessive daytime sleepiness: a feasibility study
Authors: Hlodak Jan , Geckova Andrea Madarasova , Carnakovic Simona , Feketeova Eva
Published: August 2024

Purpose

Excessive daytime sleepiness (EDS) can have a significant impact on health and quality of life but may remain undiagnosed due to low awareness and underestimation of the clinical impact of the symptoms. An online screening tool supported by media campaigns might increase awareness and help detect undiagnosed cases of EDS and narcolepsy. The aim of this study was to develop an online screening method, along with a media campaign focusing on EDS, and evaluate its feasibility.

Methods

Online screening supported by a media campaign targeting young and middle-aged adults (18–45 years old) were developed and implemented over a period of 1 year starting from November 2022. The Epworth Sleepiness Scale was used to identify EDS, and the Swiss Narcolepsy Scale was used to identify narcolepsy. In addition, the data on sociodemographic characteristics, selected sleep and health indicators and lifestyle behaviors were collected to indicate the etiology of the EDS. Feasibility, e.g., implementation and practicality, was assessed by the response rate, response to the promotion strategy, time spent on the tool, sample characteristics, and the prevalence of identified EDS and narcolepsy cases.

Results

A total of 2,390 people opened the screening link; 568 of them completed the online screening (23.8%), and most of them (n = 437, 76.9%) left their contact data to receive feedback. We identified 171 (30.1%) respondents at risk of EDS and 61 (10.7%) at risk of narcolepsy. The mean time of the screening was 15 min.

Conclusion

An online screening tool supported with a campaign seems to be a feasible way to increase awareness about EDS and prevent delayed detection of EDS cases.
There and back again: dynamics of temporary labor migration, insights from rural India
Authors: Attah-Otu Bernard , Sengupta Angan , McAleavy Tony
Published: August 2024

Temporary labor migration is a household phenomenon among rural communities in India. This study seeks to understand the subjective experiences influencing the temporariness of labor migration among internal migrants in India by examining various factors such as migration conditions, motivation, migration arrangements, coping and adaptation strategies, and determinants of stay. To achieve this objective, the current qualitative study utilized 14 in-depth interviews and 2 focus group discussions to investigate the temporary nature of labor migration among internal migrants in India. Our findings reveal that migration decisions are rational choices made collectively at the household level, considering socio-economic outcomes. We also find that social networks and contractors facilitate migration arrangements and job connections, and migrants employ various strategies to reduce costs and cope with expenses in urban areas. However, migration destinations often fail to meet migrants’ expectations, exposing them to low-wage employment and precarious working and living conditions, which are detrimental to their health. Limited housing and sanitation facilities further contribute to the challenges faced by migrants. Work conditions, including poor wages and high job demands, also affect their well-being. These findings highlight the need for improved support systems that address accommodation challenges, work conditions, and the overall welfare of labor migrants.
First case report of diagnosis of extrapancreatic solid pseudopapillary tumor with SMA invasion in a 47-year-old man: a case report and literature review
Authors: Zhang Aijing , Wang Kaibin , Tian Xiaohan , Chen Shuhai , Xu Jianwei , Liu Han , Wang Lei , Li Feng
Published: August 2024

Background

Solid pseudopapillary tumor of the pancreas (SPT) is a rare low-grade malignant tumor predominantly observed in young women without significant clinical symptoms. While most SPTs occur in the pancreatic region, rare cases have occurred in the retroperitoneum, making the diagnosis of ectopic SPTs difficult.

Case presentation

Herein, we report a rare case of an extrapancreatic solid SPT with superior mesenteric artery (SMA) involvement in a 47-year-old man together with a literature review to provide context with clinical information, CT and a literature review.

Conclusions

This case may provide a practical approach for the diagnosis of ectopic SPT, especially for patients with vascular invasion.
Case report: A mesenchymal chondrosarcoma with alternative HEY1::NCOA2 fusions in the sella turcica
Authors: Kishikawa Satsuki , Kondo Akihide , Yao Takashi , Saito Tsuyoshi
Published: August 2024

Introduction

Mesenchymal chondrosarcoma (MCS) is a rare subtype of chondrosarcoma that occurs at widespread anatomical locations, such as bone, soft tissue, and intracranial sites. The central nervous system (CNS) is one of the most common origins of extraosseous MCS. However, alternative HEY1::NCOA2 fusions have not been reported in this tumor.

Case report

We report a case of intracranial MCS with HEY1::NCOA2 rearrangement. A 52-year-old woman presented with a 15-mm calcified mass around the sella turcica. She initially underwent transsphenoidal surgery for tumor resection and then additional resections for five local recurrences over 5 years. Histologically, the tumor was composed of small round to spindle-shaped cells admixed with well-differentiated hyaline cartilaginous islands. A hemangiopericytoma-like vascular pattern and small sinusoid-like vessels were also observed. RNA sequencing using RNA extracted from formalin-fixed paraffin-embedded samples from the last operation revealed two alternative variants of the HEY1::NCOA2 fusion: HEY1(ex4)::NCOA2 (ex13) and HEY1(ex4)::NCOA2(ex14). Both variants were confirmed as in-frame fusions using reverse transcription-polymerase chain reaction.

Discussion

Cartilaginous components were often not apparent during the recurrences. In addition to the non-typical pathological finding, the correct diagnosis was hampered by the poor RNA quality of the surgical specimens and non-specific STAT6 nuclear staining.

Conclusion

This is the first reported case of intracranial MCS with an alternative HEY1::NCOA2 fusion.
Fast Minimum Error Entropy for Linear Regression
Authors: Li Qiang , Liao Xiao , Cui Wei , Wang Ying , Cao Hui , Guan Qingshu
Published: August 2024

The minimum error entropy (MEE) criterion finds extensive utility across diverse applications, particularly in contexts characterized by non-Gaussian noise. However, its computational demands are notable, and are primarily attributable to the double summation operation involved in calculating the probability density function (PDF) of the error. To address this, our study introduces a novel approach, termed the fast minimum error entropy (FMEE) algorithm, aimed at mitigating computational complexity through the utilization of polynomial expansions of the error PDF. Initially, the PDF approximation of a random variable is derived via the Gram–Charlier expansion. Subsequently, we proceed to ascertain and streamline the entropy of the random variable. Following this, the error entropy inherent to the linear regression model is delineated and expressed as a function of the regression coefficient vector. Lastly, leveraging the gradient descent algorithm, we compute the regression coefficient vector corresponding to the minimum error entropy. Theoretical scrutiny reveals that the time complexity of FMEE stands at O(n), in stark contrast to the O(n2) complexity associated with MEE. Experimentally, our findings underscore the remarkable efficiency gains afforded by FMEE, with time consumption registering less than 1‰ of that observed with MEE. Encouragingly, this efficiency leap is achieved without compromising accuracy, as evidenced by negligible differentials observed between the accuracies of FMEE and MEE. Furthermore, comprehensive regression experiments on real-world electric datasets in northwest China demonstrate that our FMEE outperforms baseline methods by a clear margin.
Soft Robotic System with Continuum Manipulator and Compliant Gripper: Design, Fabrication, and Implementation
Authors: Qaddoori Fenjan Shakir , Fathollahi Dehkordi Siavash
Published: August 2024

This paper presents the design, construction, and implementation of a soft robotic system comprising a continuum manipulator arm equipped with a compliant gripper. Three main objectives were pursued: (1) developing a soft silicone gripper as an alternative to expensive and rigid steel grippers, enabling safe and precise handling of delicate or irregular objects such as fruits, glassware, and irregular shapes; (2) fabricating a continuum manipulator arm with robotic joints inspired by vertebrae, allowing for smooth, non-linear motion and more excellent maneuverability compared to traditional rigid arms, enabling access to hard-to-reach areas; and (3) integrating the compliant gripper with the continuum manipulator and implementing a control system for the soft gripper and remote bending arm using a microcontroller. The soft gripper, manipulator arm vertebrae, and other components were fabricated using 3D printing with PLA material for the molds. The gripper construct used hyperelastic silicone (Ecoflex 00.30). The continuum manipulator achieved a higher degree of freedom and mobility, while simulations and experiments validated the design’s effectiveness. The comparison shows that the close agreements differ by only 2.5%. In practical experiments involving lifting objects, the gripper was able to carry items with a greater mass. The proposed soft, integrated robotic system outperformed traditional rigid approaches, offering safe and flexible handling capabilities in unstructured environments. The nature-inspired design enabled a compliant grip and enhanced maneuverability, making it suitable for various applications requiring dexterous manipulation of delicate or irregularly shaped objects.
Comparative Study of Methods for Robot Control with Flexible Joints
Authors: Zotovic-Stanisic Ranko , Perez-Ubeda Rodrigo , Perles Angel
Published: August 2024

Robots with flexible joints are gaining importance in areas such as collaborative robots (cobots), exoskeletons, and prostheses. They are meant to directly interact with humans, and the emphasis in their construction is not on precision but rather on weight reduction and soft interaction with humans. Well-known rigid robot control strategies are not valid in this area, so new control methods have been proposed to deal with the complexity introduced by elasticity. Some of these methods are seldom used and are unknown to most of the academic community. After selecting the methods, we carried out a comprehensive comparative study of algorithms: simple gravity compensation (Sgc), the singular perturbation method (Spm), the passivity-based approach (Pba), backstepping control design (Bcd), and exact gravity cancellation (Egc). We modeled these algorithms using MATLAB and simulated them for different stiffness levels. Furthermore, their practical implementation was analyzed from the perspective of the magnitudes to be measured and the computational costs of their implementation. In conclusion, the Sgc method is a fast and affordable solution if joint stiffness is relatively high. If good performance is necessary, the Pba is the best option.
Robustness Improved Method for Deadbeat Predictive Current Control of PMLSM with Segmented Stators
Authors: Gu Shijie , Leng Peng , Chen Qiang , Jin Yuxin , Li Jie , Yu Peichang
Published: August 2024

Permanent magnet linear synchronous motors (PMLSMs) with stator segmented structures are widely used in the design of high-power propulsion systems. However, due to the inherent delay and segmented structure of the systems, there are parameter disturbances in the inductance and flux linkage of the motors. This makes the deadbeat predictive current control (DPCC) algorithm for a current loop less robust in the control system, leading to a decrease in control performance. Compensation methods such as compensation by observer and online estimation of parameters, are problematic to apply in practice due to the difficulty of parameter adjustment and the high complexity of the algorithm. In this paper, a robustness-improved incremental DPCC (RII-DPCC) method—which uses incremental DPCC (I-DPCC) to eliminate flux linkage parameters—is proposed. The stability of the current loop was evaluated through zero-pole analysis of the discrete transfer function. Current feedforward was introduced to improve the stability of I-DPCC. The inductance stability range of I-DPCC was increased from 0.8–1.25 times to 0–2 times the actual value, and the theoretical stability range was increased more than 4 times, effectively improving the robustness of the predictive model to flux linkage and inductance parameters. Finally, the effectiveness of the proposed method was verified through numerical simulation and experiment.
The Content of Soil Glomalin Concerning Selected Indicators of Soil Fertility
Authors: Černý Jindřich , Balík Jiří , Suran Pavel , Sedlář Ondřej , Procházková Simona , Kulhánek Martin
Published: August 2024

The glomalin content is generally considered an indicator of the soil organic matter (SOM) quality. The content of easily extractable glomalin (EEG) and the total glomalin (TG) content was investigated across 71 different sites in the Czech Republic with arable soil and crop production (12 chernozems, 30 luvisols, 17 cambisols, and 12 fluvisols). The majority of the crops in the crop rotation were cereals (45.5%—mainly winter wheat, winter barley, and spring barley). The proportion of winter canola within the crop rotation was 15.9%. The contribution of other crops was substantially smaller (alfalfa, clover, potatoes, beet, silage maize, grain maize). The representation of crops in the crop rotation is standard for conventional farming in the Czech Republic. Based on the results of long-term field monitoring at 71 sites in different soil–climate conditions, we can state the following. The TG content was significantly correlated with the soil organic matter carbon content (CSOM), as well as another important indicator of SOM quality (humic and fulvic acid carbon content ratio—CHA/CFA). A significant and positive correlation was also determined for the TG and clay content (size < 0.002 mm), as well as particles smaller than 0.01 mm. The easily extractable glomalin content (EEG) did not differ based on the reference soil group (RSG). On the other hand, the total glomalin content (TG) was significantly higher in the chernozem RSG in comparison with other RSGs (luvisols, cambisols, fluvisols). There was no relationship between the pHCaCl2 and glomalin (EEG; TG). The same can be said about the relationship between glomalin (EEG; TG) and the bulk density and porosity. No link was established between the glomalin content (EEG; TG) and phosphorus plant-available content. There was no relationship between the amount of applied organic matter (carbon inputs) and the soil glomalin content (EEG; TG). This relationship was not influenced by the type of applied organic fertilizer. No significant relationship was found for either straw, manure, or compost. The data on the glomalin content are significantly influenced by the site (soil type and soil texture).
Comparative Analysis of Nature-Inspired Metaheuristic Techniques for Optimizing Phishing Website Detection
Authors: Nagunwa Thomas
Published: August 2024

The increasing number, frequency, and sophistication of phishing website-based attacks necessitate the development of robust solutions for detecting phishing websites to enhance the overall security of cyberspace. Drawing inspiration from natural processes, nature-inspired metaheuristic techniques have been proven to be efficient in solving complex optimization problems in diverse domains. Following these successes, this research paper aims to investigate the effectiveness of metaheuristic techniques, particularly Genetic Algorithms (GAs), Differential Evolution (DE), and Particle Swarm Optimization (PSO), in optimizing the hyperparameters of machine learning (ML) algorithms for detecting phishing websites. Using multiple datasets, six ensemble classifiers were trained on each dataset and their hyperparameters were optimized using each metaheuristic technique. As a baseline for assessing performance improvement, the classifiers were also trained with the default hyperparameters. To validate the genuine impact of the techniques over the use of default hyperparameters, we conducted statistical tests on the accuracy scores of all the optimized classifiers. The results show that the GA is the most effective technique, by improving the accuracy scores of all the classifiers, followed by DE, which improved four of the six classifiers. PSO was the least effective, improving only one classifier. It was also found that GA-optimized Gradient Boosting, LGBM and XGBoost were the best classifiers across all the metrics in predicting phishing websites, achieving peak accuracy scores of 98.98%, 99.24%, and 99.47%, respectively.
Dentition Anomalies and Cranial Abnormalities in Invasive Beavers (Castor canadensis) in Tierra Del Fuego, Argentina
Authors: González-Calderón Alvaro
Published: August 2024

The study of dentition anomalies and pathologies in wildlife contributes, together with other indicators, to understanding the relevance of some factors on the health status of populations. This has not been properly evaluated in invasive mammals. To test the feasibility of eradication, the government of Tierra del Fuego performed the eradication of beavers (Castor canadensis) from 2016 to 2018: 1121 animals were removed and 970 examined. These beavers were examined to analyze the incidence of dentition anomalies and other dentition and cranial conditions. The beavers presented two dentition anomalies: hypodontia and hyperdontia (0.41% in both cases), and for the first time, a dentition anomaly in the upper quadrants was reported. Beavers also presented artifactual tooth loss (0.30%) and acquired tooth loss (0.61%) but with low incidence. The presence of fractured teeth (0.41%) and caries (0.31%) occurred in all age classes, also with low incidence. The third molar tooth was the most affected, showing a female bias. The 0.93% of skulls had a cranial abnormality represented as a buccal curvature. Malocclusion cases also were recorded (0.51%). The low prevalence of dentition anomalies, fractured teeth, caries, and cranial abnormalities would not compromise the lifespan of beavers. These results leave a precedent of dentition anomalies and dentition and cranial conditions in an invasive mammal.
Density and Home Range of Cats in a Small Inhabited Mediterranean Island
Authors: Molina-Bernabeu Sara , López-Iborra Germán
Published: August 2024

There is growing concern about effectively controlling cat populations due to their impact on biodiversity, especially on islands. To plan this management, it is essential to know the cat population size, sterilization rates, and space they use. Small inhabited islands can have very high cat densities; thus, this study aimed to evaluate cat density and home range on a small tourist island in the Spanish Mediterranean. Surveys in the urban area identified individual cats using a photographic catalog, and camera trapping was conducted in the scrubland area. GPS devices were fitted on three urban cats. The overall cat density was estimated to be 308 cats/km2, varying between the urban area (1084 cats/km2) and the uninhabited scrubland (27 cats/km2). Urban cats had smaller average home ranges (0.38 ha or 1.25 ha, depending on the estimation method) compared to scrubland cats (9.53 ha). Penetration of scrubland cats into the urban area was not detected. These results indicate that the urban area acts as a source of cats for the scrubland. Although the total sterilization rate was high (90.3%), the large cat population implies that the density would take over a decade to decrease to acceptable levels. Therefore, complementary measures for managing this cat population are recommended.