bluuebunny/crossref_metadata_2025 huggingface dataset:
Fast Path Planning of Autonomous Vehicles in 3D Environments
Authors: Kim Jonghoek
Published: April 2022

Three dimensional path planner is crucial for the safe navigation of autonomous vehicles (AV), such as unmanned aerial vehicles or unmanned underwater vehicles, which operate in three dimensions. In this paper, we develop a novel 3D path planner, which is fast in generating a near-optimal solution path. The planner generates the 3D path considering the size of an AV so that as the AV traverses the constructed path, it does not collide with an obstacle. This paper introduces a 3D path planner with novel concepts, such as a virtual agent and virtual sensors. In order to generate a 3D path to the goal as fast as possible, we let the virtual agent deploy virtual sensors iteratively, such that the connected sensor network can be formed. The constructed sensor network serves as a topological map for the AV, and we find a shortest path from the start to the goal utilizing the network. The virtual agent’s maneuver is biased towards the goal, in order to find a path to the goal as fast as possible. Moreover, the size of the agent is set considering the safety margin of the generated path. Through MATLAB simulations, we demonstrate the outperformance (low computational load and short path length) of our 3D path planner by comparing it with the 3D RRT-star algorithm.
Time-Dependent Behavior of Reinforced Concrete Beams under High Sustained Loads
Authors: Shubaili Mohammed , Elawadi Ali , Orton Sarah , Tian Ying
Published: April 2022

High levels of sustained load can lead to time-dependent failure of reinforced concrete (RC) members. This in turn may lead to collapse of all or part of a building. Design errors, construction errors, and material deterioration may lead to concrete elements being subjected to high levels of sustained loads well exceeding typical service loads. Plain concrete can experience compressive failure when subjected to a high sustained stress (over 75% of its short-term strength). However, there is a lack of knowledge about the time-dependent strength and stiffness characteristics of RC members under high sustained loads. This paper presents the results of experimental testing of simply supported shear-controlled RC beams under high sustained loads. Two series of beams, consisting of 4 and 5 beams, were tested at concrete ages of 67 to 543 days to represent in-service concrete structures. The applied sustained loads ranged from 82% to 98% of the short-term capacity and lasted for 24 to 52 days. Test results indicated that high sustained load may eventually lead to failure (collapse); however, the level of load needs to be very close (~98%) to the short-term capacity. Under sustained load, all specimens experienced increased deflection with over half of the deflection increase occurring in the first 24 h. The sustained load increased the deflection at shear failure by 190% on average. The increase in the beam deflection may allow for load redistribution in redundant structural systems. A sharp increase in deflection due to tertiary creep occurred in a short time (~2 min) before failure, indicating little warning of the impending failure.
Influence of Artificial Soft Tissue on Intra-Operative Vibration Analysis Method for Primary Fixation Monitoring in Cementless Total Hip Arthroplasty
Authors: Athanassoulis Makris George , Pastrav Leonard , Goossens Quentin , Timmermans Maikel , Mulier Michiel , Vles Georges Frederic , Desmet Wim , Denis Kathleen
Published: April 2022

In cementless Total Hip Arthroplasty (THA), achieving high primary implant fixation is crucial for the long-term survivorship of the femoral stem. While orthopedic surgeons traditionally assess fixation based on their subjective judgement, novel vibration-analysis fixation-monitoring techniques show promising potential in providing the surgeon with objective and quantifiable fixation measurements. This study presents a dynamic response measurement protocol for implant endpoint insertion and evaluates this protocol in the presence of artificial soft tissue. After the artificial femur was prepared in accordance with the THA protocol, the implant was inserted and progressively hammered into the cavity. The Pearson Correlation Coefficient (PCC) and Frequency Response Assurance Criterion (FRAC) corresponding to each insertion hammer hit were derived from the Frequency Response Functions (FRF) corresponding to each insertion step. The protocol was repeated with the artificial femur submerged in artificial soft tissue to imitate the influence of anatomical soft tissue. The FRAC appeared overall more sensitive than the PCC. In the presence of the artificial soft tissue the technique yielded higher PCC and FRAC values earlier in the insertion process. The measurements with artificial soft tissue produced FRFs with fewer peaks, lower resonance frequencies, and overall higher damping factors. The soft tissue appears to limit the fixation-change detection capabilities of the system and a promising potential remedy to this limitation is suggested.
Use of Raman Spectroscopy, Scanning Electron Microscopy and Energy Dispersive X-ray Spectroscopy in a Multi-Technique Approach for Physical Characterization of Purple Urine Bag Syndrome
Authors: Acri Giuseppe , Sansotta Carlo , Salmeri Francesca Maria , Romeo Marco , Ruello Elisa V. , Denaro Lucia , Testagrossa Barbara
Published: April 2022

Purple urine bag syndrome (PUBS) is a rare condition characterized by purple discoloration of urine and urine bags. Although it is benign, it represents an alarming symptom to the patients and their relatives because of purple discoloration. We have physically characterized urine and urine bags belonging to a patient suffering from PUBS using an approach that combines Raman spectroscopy (RS) and scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX). Five “blue” discolored bags and one sterile urine bag, representing the control, were cut into 1 cm2 square samples and analyzed by using RS and SEM + EDX technique. RS enabled us to identify the presence of indigo, a metabolite of tryptophan, while SEM analysis showed the biofilm deposit, probably due to the presence of microorganisms, and the EDX measurements exhibited the elemental composition of the bags. In particular, urine bags before and after the presence of PUBS urine showed an increase of ~32% of Cl, ~33% of O, ~667% of Ca, ~65% of Al and Mg, while C decreased by about 41%. Our results, to be taken as a proof-of-principle study, are promising for the aim to characterizing the urine bags in a flexible, inexpensive, and comprehensive manner.
Development of an Automated Body Temperature Detection Platform for Face Recognition in Cattle with YOLO V3-Tiny Deep Learning and Infrared Thermal Imaging
Authors: Guo Shih-Sian , Lee Kuo-Hua , Chang Liyun , Tseng Chin-Dar , Sie Sin-Jhe , Lin Guang-Zhi , Chen Jih-Yi , Yeh Yi-Hsin , Huang Yu-Jie , Lee Tsair-Fwu
Published: April 2022

This study developed an automated temperature measurement and monitoring platform for dairy cattle. The platform used the YOLO V3-tiny (you only look once, YOLO) deep learning algorithm to identify and classify dairy cattle images. The system included a total of three layers of YOLO V3-tiny identification: (1) dairy cow body; (2) individual number (identity, ID); (3) thermal image of eye socket identification. We recorded each cow’s individual number and body temperature data after the three layers of identification, and carried out long-term body temperature tracking. The average prediction score of the recognition rate was 96%, and the accuracy was 90.0%. The thermal image of eye socket recognition rate was >99%. The area under the receiver operating characteristic curves (AUC) index of the prediction model was 0.813 (0.717–0.910). This showed that the model had excellent predictive ability. This system provides a rapid and convenient temperature measurement solution for ranchers. The improvement in dairy cattle image recognition can be optimized by collecting more image data. In the future, this platform is expected to replace the traditional solution of intrusive radio-frequency identification for individual recognition.
Sagittal Balance in Children: Reference Values of the Sacral Slope for the Roussouly Classification and of the Pelvic Incidence for a New, Age-Specific Classification
Authors: Negrini Stefano , Zaina Fabio , Cordani Claudio , Donzelli Sabrina
Published: April 2022

Background: The Roussouly classification, based on the functional parameter sacral slope (SS), describes the normal sagittal balance in adults and has proved useful for surgery. Reference values in children should be defined, since they are an important treatment target of conservative treatment. Moreover, during growth, there are few correlations between sagittal parameters, and we hypothesize that a new classification based on the anatomical parameter pelvic incidence (PI) could also be useful. We performed a cross-sectional study to identify the reference values for the Roussouly classification during growth and to develop a new classification based on PI in children. Methods: Correlations between sagittal parameters and age were searched in 222 healthy subjects at the first consultation (6–18 years old). A new classification, based on PI, and comprising three types, is defined and compared to the Roussouly classification. Results: With age, correlations among sagittal balance parameters increase, as well as SS and PI, but with different cut-offs. The distribution of Roussouly types do not correspond to that in adulthood; thus, we defined new reference cut-offs. We defined a PI-based classification in three types, not overlapping Roussouly’s. We found a uniform and balanced distribution of cases among the nine possible combinations. Conclusions: In children, we need to use new thresholds for the Roussouly types. The new classification based on PI is correlated with the Roussouly classification, but it is also clearly different. Future studies will determine its validity.
Time-Varying Degradation Model for Remaining Useful Life Prediction of Rolling Bearings under Variable Rotational Speed
Authors: Du Wenliao , Hou Xukun , Wang Hongchao
Published: April 2022

It is difficult to accurately extract the health index of non-stationary signals of rolling bearings under variable rotational speed, which also leads to greater prediction error for bearing degradation models with fixed parameters. For this reason, an angular domain unscented particle filter model with time-varying degradation parameters is proposed to deal with the remaining useful life (RUL) prediction of rolling bearings. Order analysis is first performed to transform the variable-speed signal from time domain to angular domain for extracting the health index in the angular domain representation. To track the bearing degradation state, a real-time finite element model is established to guide the parameters updating the procedure of the performance degradation model. Finally, the bearing degradation state is estimated by the unscented particle filter (UPF), and then the remaining useful life of the bearing is predicted. In this way, the time-varying degradation model is developed by considering both non-stationary feature extraction and dynamic state tracking for rolling bearings. The proposed method is verified by both benchmarks: bearing experimental data, and a bearing accelerated life experiment. Compared with state-of-the-art prognostic methods, the present model can predict the bearing remaining useful life (RUL) more accurately under variable rotational speed.
Experimental Study of a Rotating Electrode Plasma Reactor for Hydrogen Production from Liquid Petroleum Gas Conversion
Authors: Barkhordari Ali , Mirzaei Seyed Iman , Falahat Amir , Krawczyk Dorota A. , Rodero Antonio
Published: April 2022

In this work, a new plasma reactor operating with a butane/propane (C4H10/C3H8) gaseous mixture, designed for hydrogen molecule production, was experimentally studied. This reactor is based on a rotating electrode, biased by an AC high voltage. The plasma discharge was investigated for different AC voltages, rotational frequencies, and gas flow rates. A discharge in the filamentary mode was produced as proved by the electrical characterization. Gas Chromatography (GC) was applied to study the LPG remediation. The maximum conversion factors of 70% and 60% were found for the C3H8 and C4H10, respectively, with an H2 selectivity of 98%. Hydrogen atomic lines from the Balmer series and various molecular bands were detected by optical emission spectroscopy (OES). The stark broadening of the Hα Balmer line was used for the determination of the electron density. The spectra simulation of the C2 band was permitted to obtain the gas temperature while the first five lines of hydrogen atoms were used to calculate the electron temperature. A non-equilibrium plasma with two very different temperatures for electrons and heavy particles was found. The spectroscopic study allowed us to explain the experimental results of the LPG conversion and its dependence on the plasma conditions, resulting in optimizing the H2 formation.
The Influence of Pore Distribution of Coal Char in the Char Fragmentation and Included Minerals Partitioning: A Percolation Modeling
Authors: Li Rui , Jing Zhenqi , Ma Jingjing , Qin Long , Yan Kai , Wen Chang
Published: April 2022

The processes of char fragmentation, including mineral partitioning and particulate matter (PM) formation during dense and porous char combustion, were observed by a site percolation model. This model simulated the diffusion-controlled regime of char combustion, and the size distributions of included minerals in typical bituminous coal were determined by the computer-controlled scanning electron microscope (CCSEM), and the data were put into the char matrix randomly. The model presents the influence of initial pore distribution on char oxidation and fragmentation, the impact of the char conversion process on the extent of fragmentation, the change of ash distributions with the char conversion, and the particulate matters (PM) size distribution, which is derived from the consequence of the competition between char fragmentation and included minerals partitioning and coalescence. The results indicate that with increasing initial char porosity (φ), the number of large size pores increases but the number of pores decreases, which leads to open pores increasing, close pores decreasing, and the surface reaction area increasing. While φ ≥ 0.4, char fragmentation obviously occurs during the stage in which the rates of char conversion are 0.4–0.6, and it looks as though the maximum value of fragmentation will transfer to an earlier conversion stage if it has a larger φ. The enhanced φ shows a positive effect on the increase in the number and concentration of PM < 10 μm (nominally aerodynamic diameter), this is attributed to char fragments more drastically, and the probability of mineral coalescence reduces a lot.
MesSBAR—Multicopter and Instrumentation for Air Quality Research
Authors: Bretschneider Lutz , Schlerf Andreas , Baum Anja , Bohlius Henning , Buchholz Marcel , Düsing Sebastian , Ebert Volker , Erraji Hassnae , Frost Paul , Käthner Ralf , Krüger Thomas , Lange Anne Caroline , Langner Marcel , Nowak Andreas , Pätzold Falk , Rüdiger Julian , Saturno Jorge , Scholz Hendrik , Schuldt Tobias , Seldschopf Rickmar , Sobotta Andre , Tillmann Ralf , Wehner Birgit , Wesolek Christian , Wolf Katharina , Lampert Astrid
Published: April 2022

Air quality measurements usually consist of ground-based instrumentation at fixed locations. However, vertical profiles of pollutants are of interest for understanding processes, distribution, dilution and concentration. Therefore, a multicopter system has been developed to investigate the vertical distribution of the concentration of aerosol particles, black carbon, ozone, nitrogen oxides (NOx) and carbon monoxide and the meteorological parameters of temperature and humidity. This article presents the requirements by different users, the setup of the quadrocopter system, the instrumentation and the results of first applications. The vertical distribution of particulate matter next to a highway was strongly related to atmospheric stratification, with different concentrations below and above the temperature inversion present in the morning. After the qualification phase described in this article, two identically equipped multicopters will be used upwind and downwind of line or diffuse sources such as highways or urban areas to quantify the influence of their emissions on the local air quality.
Impact of Climate Change on the Yield and Water Footprint of Winter Wheat in the Haihe River Basin, China
Authors: Jia Dongdong , Wang Chunying , Han Yuping , Huang Huiping , Xiao Heng
Published: April 2022

Climate change can impact the yield and water footprint of crops. Therefore, assessing such impacts carries great significance for regional water and food security. This study validated and verified the variety parameters of winter wheat for the Decision Support System for Agrotechnology Transfer (DSSAT) model, using the long-term (1993–2013) growth and yield data observed from six agricultural experiment stations in the Haihe River Basin (HRB), China. The growth process was simulated under three representative concentration pathways (RCPs), named RCP2.6, RCP4.5, and RCP8.5—climate scenarios driven by the HadGEM2-ES model. The variety parameters of winter wheat showed high accuracy in the simulation of the anthesis and maturity dates, and could be used for long-term prediction of the growth process. The trends of climate change had positive impacts on the water footprint of winter wheat but adverse impacts on the yield. The growing period was shortened by 3.6 days, 4.7 days, and 5.0 days per decade in the RCP2.6, RCP4.5, and RCP8.5 scenarios, respectively, due to the rapid accumulation of heat. The yield would be increased in lower emissions scenarios (17% in RCP2.6), but decreased in high-emissions scenarios due to high temperatures, which may restrict the growth of wheat. The water footprint was decreased by 10%, 11%, and 13% in the RCP2.6, RCP4.5, and RCP8.5 scenarios, respectively, indicating that the water-use efficiency could be improved in the future. The results showed broad application prospects of the DSSAT model in simulating the response of crop growth to climate change.
Long-Term Exposure to Essential Oils and Cardiopulmonary Health from a Population-Based Study
Authors: Lee Chien-Yu , Lin Lian-Yu , Chuang Hsiao-Chi , Ho Kin-Fai , Chuang Kai-Jen
Published: April 2022

It is still unknown whether long-term inhalation of indoor air pollutants from ambient essential oil is associated with increased cardiopulmonary events. We recruited 200 healthy homemakers to conduct a prospective observation study in Northern Taiwan. We measured heart rate (HR), systolic blood pressure (SBP), diastolic BP (DBP), peak expiratory flow rate (PEFR), and indoor air pollutants four times per year for each participant between 2008 and 2018. Moreover, a questionnaire related to essential oil usage, home characteristics, and health status was filled out with each participant. The association between essential oil usage and cardiopulmonary health was determined using mixed-effects models. The mixed-effects models showed a significant association between essential oil usage and adverse cardiopulmonary effects including increased HR and BP and decreased % predicted PEFR among participants with heavy use of essential oils. No significant association between essential oils usage and adverse cardiopulmonary effects was observed among participants without essential oils usage or participants with mild use of essential oils (less than one hour per day). We concluded that exposure to indoor air pollution related to essential oils was associated with adverse cardiopulmonary effects among participants with essential oil usage more than one hour per day.
Estimation of the Near-Surface Ozone Concentration with Full Spatiotemporal Coverage across the Beijing-Tianjin-Hebei Region Based on Extreme Gradient Boosting Combined with a WRF-Chem Model
Authors: Hu Xiaomin , Zhang Jing , Xue Wenhao , Zhou Lihua , Che Yunfei , Han Tian
Published: April 2022

With the intensification of global warming and economic development in China, the near-surface ozone (O3) concentration has been increasing recently, especially in the Beijing-Tianjin-Hebei (BTH) region, which is the political and economic center of China. However, O3 has been measured in real time only over the past few years, and the observational records are discontinuous. Therefore, we propose a new method (WRFC-XGB) to establish a near-surface O3 concentration dataset in the BTH region by integrating the Weather Research and Forecasting with Chemistry (WRF-Chem) model with the extreme gradient boosting (XGBoost) algorithm. Based on this method, the 8-h maximum daily average (MDA8) O3 concentrations are obtained with full spatiotemporal coverage at a spatial resolution of 0.1° × 0.1° across the BTH region in 2018. Two evaluation methods, sample- and station-based 10-fold cross-validation (10-CV), are used to assess our method. The sample-based (station-based) 10-CV evaluation results indicate that WRFC-XGB can achieve excellent accuracy with a high coefficient of determination (R2) of 0.95 (0.91), low root mean square error (RMSE) of 13.50 (17.70) µg m−3, and mean absolute error (MAE) of 9.60 (12.89) µg m−3. In addition, superb spatiotemporal consistencies are confirmed for this model, including the estimation of high O3 concentrations, and our WRFC-XGB model outperforms traditional models and previous studies in data mining. In addition, the proposed model can be applied to estimate the O3 concentration when it has not been measured. Furthermore, the spatial distribution analysis of the MDA8 O3 in 2018 reveals that O3 pollution in the BTH region exhibits significant seasonality. Heavy O3 pollution episodes mainly occur in summer, and the high O3 loading is distributed mainly in the southern BTH areas, which will pose challenges to atmospheric environmental governance for local governments.
A Comparative Study of Models for Heat Transfer in Bidisperse Gas–Solid Systems via CFD–DEM Simulations
Authors: Huang Zheqing , Huang Qi , Yu Yaxiong , Li Yu , Zhou Qiang
Published: April 2022

In this study, flow and heat transfers in bidisperse gas–solid systems were numerically investigated using the computational fluid dynamics–discrete element method (CFD–DEM). Three different models to close the gas–solid heat transfer coefficient for each species of bidisperse systems were compared in the simulations. The effect of the particle diameter ratio and particle number ratio between large and small particles on the particle mean temperature and temperature distribution of each species were systematically investigated. The simulation results show that differences in the particle mean temperature and temperature distribution profiles exist among the three heat transfer models at a higher particle number ratio. The differences between the effects of three heat transfer models on heat transfer properties in bidisperse systems with particle diameter ratios of up to 4 are marginal when the particle number ratio between small and large particles is 1.
Revisiting Gradient Boosting-Based Approaches for Learning Imbalanced Data: A Case of Anomaly Detection on Power Grids
Authors: Louk Maya Hilda Lestari , Tama Bayu Adhi
Published: April 2022

Gradient boosting ensembles have been used in the cyber-security area for many years; nonetheless, their efficacy and accuracy for intrusion detection systems (IDSs) remain questionable, particularly when dealing with problems involving imbalanced data. This article fills the void in the existing body of knowledge by evaluating the performance of gradient boosting-based ensembles, including gradient boosting machine (GBM), extreme gradient boosting (XGBoost), LightGBM, and CatBoost. This paper assesses the performance of various imbalanced data sets using the Matthew correlation coefficient (MCC), area under the receiver operating characteristic curve (AUC), and F1 metrics. The article discusses an example of anomaly detection in an industrial control network and, more specifically, threat detection in a cyber-physical smart power grid. The tests’ results indicate that CatBoost surpassed its competitors, regardless of the imbalance ratio of the data sets. Moreover, LightGBM showed a much lower performance value and had more variability across the data sets.
An Emergency Event Detection Ensemble Model Based on Big Data
Authors: Alfalqi Khalid , Bellaiche Martine
Published: April 2022

Emergency events arise when a serious, unexpected, and often dangerous threat affects normal life. Hence, knowing what is occurring during and after emergency events is critical to mitigate the effect of the incident on humans’ life, on the environment and our infrastructures, as well as the inherent financial consequences. Social network utilization in emergency event detection models can play an important role as information is shared and users’ status is updated once an emergency event occurs. Besides, big data proved its significance as a tool to assist and alleviate emergency events by processing an enormous amount of data over a short time interval. This paper shows that it is necessary to have an appropriate emergency event detection ensemble model (EEDEM) to respond quickly once such unfortunate events occur. Furthermore, it integrates Snapchat maps to propose a novel method to pinpoint the exact location of an emergency event. Moreover, merging social networks and big data can accelerate the emergency event detection system: social network data, such as those from Twitter and Snapchat, allow us to manage, monitor, analyze and detect emergency events. The main objective of this paper is to propose a novel and efficient big data-based EEDEM to pinpoint the exact location of emergency events by employing the collected data from social networks, such as “Twitter” and “Snapchat”, while integrating big data (BD) and machine learning (ML). Furthermore, this paper evaluates the performance of five ML base models and the proposed ensemble approach to detect emergency events. Results show that the proposed ensemble approach achieved a very high accuracy of 99.87% which outperform the other base models. Moreover, the proposed base models yields a high level of accuracy: 99.72%, 99.70% for LSTM and decision tree, respectively, with an acceptable training time.
Effects of Chlorophyll a and b in Reducing Genotoxicity of 2-Amino-3,8-dimethylimidazo[4,5-F]quinoxaline (MeIQx)
Authors: Kocaoğlu Cenkci Serap , Kaya Bülent
Published: April 2022

In this study, the protective effects of chlorophyll a and chlorophyll b (0.5 and 1 µM) against the heterocyclic amine compound 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx, 4.69 µM, 9.38 µM, 23.45 µM) with somatic mutation and recombination test in Drosophila melanogaster are investigated. Chronic applications are performed to transheterozygous larvae with respect to two recessive genes, mwh (multiple wing hair) and flr3 (flare), by using Drosophila strains. The genotoxic effects of MeIQx are primarily determined for third instars larvae. In antigenotoxicity studies, two different application groups are constituted. While for the first group doses of chlorophyll a, b, and MeIQx are given to the third instars larvae simultaneously, for the second group doses of MeIQx are applied at the third instars after doses of chlorophyll a and b are given to at the second instars larvae. Chlorophyll a and b are effective in reducing genotoxic effects of MeIQx by both applications on individuals and it is observed that the pretreatment method is much more effective than the simultaneous one. There are similar results for chlorophyll a and b in efficacy.
The Effect of Hypothermia and Osmotic Shock on the Electrocardiogram of Adult Zebrafish
Authors: Arel Elodie , Rolland Laura , Thireau Jérôme , Torrente Angelo Giovanni , Bechard Emilie , Bride Jamie , Jopling Chris , Demion Marie , Le Guennec Jean-Yves
Published: April 2022

The use of zebrafish to explore cardiac physiology has been widely adopted within the scientific community. Whether this animal model can be used to determine drug cardiac toxicity via electrocardiogram (ECG) analysis is still an ongoing question. Several reports indicate that the recording configuration severely affects the ECG waveforms and its derived-parameters, emphasizing the need for improved characterization. To address this problem, we recorded ECGs from adult zebrafish hearts in three different configurations (unexposed heart, exposed heart, and extracted heart) to identify the most reliable method to explore ECG recordings at baseline and in response to commonly used clinical therapies. We found that the exposed heart configuration provided the most reliable and reproducible ECG recordings of waveforms and intervals. We were unable to determine T wave morphology in unexposed hearts. In extracted hearts, ECG intervals were lengthened and P waves were unstable. However, in the exposed heart configuration, we were able to reliably record ECGs and subsequently establish the QT-RR relationship (Holzgrefe correction) in response to changes in heart rate.
Genetic Diversity Trends in the Cultivated Potato: A Spatiotemporal Overview
Authors: Spanoghe Martin , Marique Thierry , Nirsha Alexandra , Esnault Florence , Lanterbecq Deborah
Published: April 2022

We investigated the changes in genetic diversity over time and space of the cultivated potato (Solanum tuberosum L.) for the period pre-1800 to 2021. A substantial panel of 1219 potato varieties, belonging to different spatiotemporal groups, was examined using a set of 35 microsatellite markers (SSR). Genotypic data covering a total of 407 alleles was analyzed using both self-organizing map (SOM) and discriminant analysis of principal components (DAPC) de novo and a priori clustering methods, respectively. Data analysis based on different models of genetic structuring provided evidence of (1) at least two early lineages that have been maintained since their initial introduction from the Andes into Europe in the 16th century, followed by later ones coming from reintroduction events from the US in the mid-1800s; (2) a level of diversity that has gradually evolved throughout the studied time periods and areas, with the most modern variety groups encompassing most of the diversity found in earlier decades; (3) the emergence of new genetic groups within the current population due to increases in the use of germplasm enhancement practices using exotic germplasms. In addition, analysis revealed significant genetic differentiation both among and within the spatiotemporal groups of germplasm studied. Our results therefore highlight that no major genetic narrowing events have occurred within the cultivated potato over the past three centuries. On the contrary, the genetic base shows promising signs of improvement, thanks to extensive breeding work that is gaining momentum. This overview could be drawn on not only to understand better how past decisions have impacted the current genetic cultivated potato resources, but also to develop appropriate new strategies for breeding programs consistent with the socio-economic and sustainability challenges faced by agrifood systems.
Epidemiology of HCV and HBV in a High Endemic Area of Southern Italy: Opportunities from the COVID-19 Pandemic—Standardized National Screening or One Tailored to Local Epidemiology?
Authors: Nevola Riccardo , Messina Vincenzo , Marrone Aldo , Coppola Nicola , Rescigno Carolina , Esposito Vincenzo , Sangiovanni Vincenzo , Claar Ernesto , Pisaturo Mariantonietta , Fusco Francesco Maria , Rosario Pietro , Izzi Antonio , Pisapia Raffaella , Rosato Valerio , Maggi Paolo , Adinolfi Luigi Elio
Published: April 2022

The COVID-19 pandemic led to the hospitalization of an unselected population with the possibility to evaluate the epidemiology of viral hepatitis. Thus, a retrospective multicenter study was conducted in an area of Southern Italy with the aim of assessing the prevalence of HCV and HBV markers and the ability of current screening program to capture cases. We evaluated 2126 hospitalized patients in seven COVID Centers of Naples and Caserta area in which 70% of the Campania population lives. HBsAg and HCV-Ab prevalence was 1.6% and 5.1%, respectively, with no differences between gender. Decade distribution for birth year shows a bimodal trend of HCV prevalence, with a peak (11.6%) in the decade 1930–1939 and a second peak (5.6%) for those born in 1960–1969. An analysis of the screening period imposed by the Italian government for those born between 1969 and 1989 shows that only 17% of cases of HCV infection could be captured. A small alignment of the screening period, i.e., those born from 1960 to 1984, would capture 40% of cases. The data confirm the high endemicity of our geographical area for hepatitis virus infections and underline the need for a tailored screening program according to the regional epidemiology.
The Fibrinogen-like Domain of ANGPTL3 Facilitates Lipolysis in 3T3-L1 Cells by Activating the Intracellular Erk Pathway
Authors: Bini Simone , Pecce Valeria , Di Costanzo Alessia , Polito Luca , Ghadiri Ameneh , Minicocci Ilenia , Tambaro Federica , Covino Stella , Arca Marcello , D’Erasmo Laura
Published: April 2022

Background: ANGPTL3 stimulates lipolysis in adipocytes, but the underlying molecular mechanism is yet unknown. The C-terminal fibrinogen-like domain of ANGPTL3 (ANGPTL3-Fld) activates the AKT pathway in endothelial cells. Hence, we evaluated whether ANGPTL3-Fld stimulates lipolysis in adipocytes through the MAPK kinase pathway. Materials and Methods: 3T3-L1 adipocytes were treated with isoproterenol (ISO), ANGPTL3-Fld, or both. Lipolysis was evaluated through the release of free fatty acids (FFAs) in the culture medium. The activation status of intracellular kinases was evaluated with and without the inhibition of the BRAF–ERK arm of the MAPK pathway. Results: ANGPTL3-Fld alone was not able to activate lipolysis, while the combination of ANGPTL3-Fld and ISO determined a 10-fold enrichment of the FFA concentration in the culture medium with an incremental effect (twofold) when compared with ISO alone. ANGPTL3-Fld alone inhibited hormone-sensitive lipase (HSL), whereas the treatment with ISO induced the activation of HSL. The net balance of ANGPTL3-Fld and ISO cotreatment resulted in HSL activation. The results indicate that ANGPTL3-Fld generated an intracellular activation signal involving the MAPK–ERK pathway, possibly through the PDGFRβ—PLCγ-AMPK axis. Conclusion: ANGPTL3-Fld appears to act as a facilitator of lipolysis in adipocytes, and this effect was driven by a signal mediated by a pathway that is different from the canonical β-adrenergic stimulus.
Expression of Beta-Catenin, Cadherins and P-Runx2 in Fibro-Osseous Lesions of the Jaw: Tissue Microarray Study
Authors: Pannone Giuseppe , Nocini Riccardo , Santoro Angela , Spirito Francesca , Nocini Pier Francesco , Papagerakis Silvana , Franceschi Renny T. , Di Domenico Marina , Di Carlo Angelina , Danelia Nana , Lo Muzio Lorenzo
Published: April 2022

Fibrous dysplasia (FD) and hyperparathyroidism-jaw tumor syndrome (HPT-JT) are well-characterized benign bone fibro-osseous lesions. The intracellular mechanism leading to excessive deposition of fibrous tissue and alteration of differentiation processes leading to osteomalacia have not yet been fully clarified. Tissue Microarray (TMA)-based immunohistochemical expression of β-catenin, CK-AE1/AE3, Ki-67, cadherins and P-Runx2 were analyzed in archival samples from nine patients affected by FD and HPT-JT and in seven controls, with the aim of elucidating the contribution of these molecules (β-catenin, cadherins and P-Runx2) in the osteoblast differentiation pathway. β-catenin was strongly upregulated in FD, showing a hyper-cellulated pattern, while it was faintly expressed in bone tumors associated with HPT-JT. Furthermore, the loss of expression of OB-cadherin in osteoblast lineage in FD was accompanied by N-cadherin and P-cadherin upregulation (p < 0.05), while E-cadherin showed a minor role in these pathological processes. P-Runx2 showed over-expression in six out of eight cases of FD and stained moderately positive in the rimming lining osteoblasts in HPT-JT syndrome. β-catenin plays a central role in fibrous tissue proliferation and accompanies the lack of differentiation of osteoblast precursors in mature osteoblasts in FD. The study showed that the combined evaluation of the histological characteristics and the histochemical and immunohistochemical profile of key molecules involved in osteoblast differentiation are useful in the diagnosis, classification and therapeutic management of fibrous-osseous lesions.
Berry Anthocyanins in Rodent and Human Obesity and Diabetes: A Review of the Evidence
Authors: Riordan Joseph , Solverson Patrick
Published: April 2022

Obesity in America is a public health crisis that will continue to impact the country at an individual, social, and economic level unless we address the disease with dietary modifications to reduce or prevent its development. Nutritional interventions designed for obesity treatment are constantly evolving. Berries, which are a rich source of polyphenols, have been suggested as a potential bioactive component, as they have been reported to have anti-obesity effects. Therefore, this review will provide an overview of epidemiological studies to introduce the idea of berries for health promotion. Studies conducted in both rodents and humans are summarized. This review includes an overview of the physiological responses associated with berry consumption, including the effects on the composition of the gut microbiota in humans and rodents, which demonstrate how berry consumption may provide a protective effect against obesity and its related comorbidities. However, these findings have yet to be translated into feasible, long-term nutrition intervention in humans. Future research into different berries and their components will identify effective, accessible functional food options that can augment nutritional interventions.
Advanced Imaging in Cardiac Amyloidosis
Authors: Waldmeier Dominik , Herzberg Jan , Stephan Frank-Peter , Seemann Marcus , Arenja Nisha
Published: April 2022

This review serves as a synopsis of multimodality imaging in cardiac amyloidosis (CA), which is a disease characterized by deposition of misfolded protein fragments in the heart. It emphasizes and summarizes the diagnostic possibilities and their prognostic values. In general, echocardiography is the first diagnostic tool in patients with an identified systemic disease or unclear left ventricular hypertrophy. Several echocardiographic parameters will raise suspicion and lead to further testing. Cardiac magnetic resonance and scintigraphy with bone avid radiotracers are crucial for diagnosis of CA and even enable a distinction between different subtypes. The subject is illuminated with established guidelines and innovative recent publications to further improve early diagnosis of cardiac amyloidosis in light of current treatment options.
Investigating the Effects of Conditioned Media from Stem Cells of Human Exfoliated Deciduous Teeth on Dental Pulp Stem Cells
Authors: Vu Huong Thu , Han Mi-Ran , Lee Jun-Haeng , Kim Jong-Soo , Shin Ji-Sun , Yoon Ji-Young , Park Jeong-Hui , Dashnyam Khandmaa , Knowles Jonathan Campbell , Lee Hae-Hyoung , Kim Jong-Bin , Lee Jung-Hwan
Published: April 2022

Pulp regeneration has recently attracted interest in modern dentistry. However, the success ratio of pulp regeneration is low due to the compromising potential of stem cells, such as their survival, migration, and odontoblastic differentiation. Stem cells from human exfoliated deciduous teeth (SHED) have been considered a promising tool for regenerative therapy due to their ability to secrete multiple factors that are essential for tissue regeneration, which is achieved by minimally invasive procedures with fewer ethical or legal concerns than those of other procedures. The aim of this study is to investigate the potency of SHED-derived conditioned media (SHED CM) on dental pulp stem cells (DPSCs), a major type of mesenchymal stem cells for dental pulp regeneration. Our results show the promotive efficiency of SHED CM on the proliferation, survival rate, and migration of DPSCs in a dose-dependent manner. Upregulation of odontoblast/osteogenic-related marker genes, such as ALP, DSPP, DMP1, OCN, and RUNX2, and enhanced mineral deposition of impaired DPSCs are also observed in the presence of SHED CM. The analysis of SHED CM found that a variety of cytokines and growth factors have positive effects on cell proliferation, migration, anti-apoptosis, and odontoblast/osteogenic differentiation. These findings suggest that SHED CM could provide some benefits to DPSCs in pulp regeneration.
Expression and Prognostic Implication of PD-L1 in Patients with Urothelial Carcinoma with Variant Histology (Squamous Differentiation or Micropapillary) Undergoing Radical Cystectomy
Authors: Chung Jae-Hoon , Lee Chung-Un , Lee Dong-Hyeon , Song Wan
Published: April 2022

The expression and prognostic role of programmed death ligand-1 (PD-L1) on tumor-infiltrating immune cells (TICs) has not been determined in urothelial carcinoma (UC) with variant histology. We retrospectively reviewed 90 patients (44 with micropapillary variant of UC (MPUC) and 46 with UC with squamous differentiation (UCSD)) who underwent radical cystectomy between January 2013 and December 2019. The expression of PD-L1 in TICs was measured using the VENTANA (SP-142) immunohistochemistry assay and dichotomized using a 5% cutoff value (positive ≥ 5%). Kaplan–Meier survival analysis was used to estimate recurrence-free survival (RFS), and multivariable Cox proportional hazard models were used to identify factors predicting tumor recurrence. Overall, positive PD-L1 expression in TICs was confirmed in 50 of 90 (55.6%) patients (40.1% (18/44) of MPUC and 69.9% (32/46) of UCSD). RFS was significantly shorter in patients with positive PD-L1 expression in TICs than in those with negative PD-L1 expression both in MPUC (p = 0.005) and UCSD (p = 0.046). Positive PD-L1 expression in TICs was significantly associated with an increased risk of tumor recurrence in both MPUC (HR = 1.85; 95% CI: 1.323–2.672; p = 0.017) and UCSD (HR = 1.58; 95% CI: 1.162–2.780; p = 0.032). In conclusion, positive PD-L1 expression in TICs was significantly associated with poorer RFS in both MPUC and UCSD patients. Our results support the use of adjuvant immunotherapy in these patients if they test positive for PD-L1 in their TICs.
Prolonged Cadmium Exposure Alters Migration Dynamics and Increases Heterogeneity of Human Uterine Fibroid Cells—Insights from Time Lapse Analysis
Authors: Yan Yitang , Shi Min , Fannin Rick , Yu Linda , Liu Jingli , Castro Lysandra , Dixon Darlene
Published: April 2022

Cadmium (Cd) is one of the most prevalent environmental heavy metal contaminants and is considered an endocrine disruptor and carcinogen. In women with uterine fibroids, there is a correlation between blood Cd levels and fibroid tumor size. In this study, fibroid cells were exposed to 10 µM CdCl2 for 6 months and a fast-growing Cd-Resistant Leiomyoma culture, termed CR-LM6, was recovered. To characterize the morphological and mechanodynamic features of uterine fibroid cells associated with prolonged Cd exposure, we conducted time lapse imaging using a Zeiss confocal microscope and analyzed data by Imaris and RStudio. Our experiments recorded more than 64,000 trackable nuclear surface objects, with each having multiple parameters such as nuclear size and shape, speed, location, orientation, track length, and track straightness. Quantitative analysis revealed that prolonged Cd exposure significantly altered cell migration behavior, such as increased track length and reduced track straightness. Cd exposure also significantly increased the heterogeneity in nuclear size. Additionally, Cd significantly increased the median and variance of instantaneous speed, indicating that Cd exposure results in higher speed and greater variation in motility. Profiling of mRNA by NanoString analysis and Ingenuity Pathway Analysis (IPA) strongly suggested that the direction of gene expression changes due to Cd exposure enhanced cell movement and invasion. The altered expression of extracellular matrix (ECM) genes such as collagens, matrix metallopeptidases (MMPs), secreted phosphoprotein 1 (SPP1), which are important for migration contact guidance, may be responsible for the greater heterogeneity. The significantly increased heterogeneity of nuclear size, speed, and altered migration patterns may be a prerequisite for fibroid cells to attain characteristics favorable for cancer progression, invasion, and metastasis.
Preparation of Graphene Quantum Dots by Visible-Fenton Reaction and Ultrasensitive Label-Free Immunosensor for Detecting Lipovitellin of Paralichthys Olivaceus
Authors: Yang Ailing , Su Yue , Zhang Zhenzhong , Wang Huaidong , Qi Chong , Ru Shaoguo , Wang Jun
Published: April 2022

The increasing levels of environmental estrogens are causing negative effects on water, soil, wildlife, and human beings; label-free immunosensors with high specificities and sensitivities are being developed to test estrogeneous chemicals in complex environmental conditions. For the first time, highly fluorescent graphene quantum dots (GQDs) were prepared using a visible-Fenton catalysis reaction with graphene oxide (GO) as a precursor. Different microscopy and spectroscopy techniques were employed to characterize the physical and chemical properties of the GQDs. Based on the fluorescence resonance energy transfer (FRET) between amino-functionalized GQDs conjugated with anti-lipovitellin monoclonal antibodies (Anti-Lv-mAb) and reduced graphene oxide (rGO), an ultrasensitive fluorescent “ON-OFF” label-free immunosensor for the detection of lipovitellin (Lv), a sensitive biomarker derived from Paralichthys olivaceus for environmental estrogen, has been established. The immunosensor has a wide linear test range (0.001–1500 ng/mL), a lower limit of detection (LOD, 0.9 pg/mL), excellent sensitivity (26,407.8 CPS/(ng/mL)), and high selectivity and reproducibility for Lv quantification. The results demonstrated that the visible-Fenton is a simple, mild, green, efficient, and general approach to fabricating GQDs, and the fluorescent “ON-OFF” immunosensor is an easy-to-use, time-saving, ultrasensitive, and accurate detection method for weak estrogenic activity.
An iEEG Recording and Adjustable Shunt-Current Conduction Platform for Epilepsy Treatment
Authors: You Changhua , Yao Lei , Yao Pan , Li Li , Ding Ping , Liang Shuli , Liu Chunxiu , Xue Ning
Published: April 2022

This paper proposes a compact bioelectronics sensing platform, including a multi-channel electrode, intracranial electroencephalogram (iEEG) recorder, adjustable galvanometer, and shunt-current conduction circuit pathway. The developed implantable electrode made of polyurethane-insulated stainless-steel materials is capable of recording iEEG signals and shunt-current conduction. The electrochemical impedance of the conduction, ground/reference, and working electrode were characterized in phosphate buffer saline solution, revealing in vitro results of 517.2 Ω@1 kHz (length of 0.1 mm, diameter of 0.8 mm), 1.374 kΩ@1 kHz (length of 0.3 mm, diameter of 0.1 mm), and 3.188 kΩ@1 kHz (length of 0.1 mm, diameter of 0.1 mm), respectively. On-bench measurement of the system revealed that the input noise of the system is less than 2 μVrms, the signal frequency bandwidth range is 1 Hz~10 kHz, and the shunt-current detection range is 0.1~3000 μA with an accuracy of above 99.985%. The electrode was implanted in the CA1 region of the right hippocampus of rats for the in vivo experiments. Kainic acid (KA)-induced seizures were detected through iEEG monitoring, and the induced shunt-current was successfully measured and conducted out of the brain through the designed circuit-body path, which verifies the potential of current conduction for the treatment of epilepsy.