bluuebunny/crossref_metadata_2025 huggingface dataset:
Co-Occurring X-Linked Agammaglobulinemia and X-Linked Chronic Granulomatous Disease: Two Isolated Pathogenic Variants in One Patient
Authors: Gunderman Lauren , Brown Jeffrey , Chaudhury Sonali , O’Gorman Maurice , Fuleihan Ramsay , Khanolkar Aaruni , Ahmed Aisha
Published: March 2023

We present a unique and unusual case of a male patient diagnosed with two coexisting and typically unassociated X-linked conditions: he was initially diagnosed with X-linked agammaglobulinemia (XLA) followed by a diagnosis of X-linked chronic granulomatous disease (XCGD) and an as of yet unpublished hypomorphic gp91phox variant in the CYBB gene. The latter was tested after the finding of granulomatous gingivitis. Hematopoietic stem cell transplant (HSCT) was performed due to severe colitis and nodular regenerative hyperplasia (NRH) of the liver. Following transplant, complete donor engraftment was observed with the restoration of a normal oxidative burst and full restoration of normal levels of circulating, mature CD19+ B cells. This case is singular in that it does not involve a contiguous gene syndrome in which deleted genes are in close proximity to either BTK and CYBB, which has been previously reported. To our knowledge, this is the first reported case of XLA and XCGD co-existing in a single patient and of having both inborn errors of immunity successfully treated by HSCT.
In Silico Identification of Lead Compounds for Pseudomonas Aeruginosa PqsA Enzyme: Computational Study to Block Biofilm Formation
Authors: Shahab Muhammad , Danial Muhammad , Khan Taimur , Liang Chaoqun , Duan Xiuyuan , Wang Daixi , Gao Hanzi , Zheng Guojun
Published: March 2023

Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium implicated in acute and chronic nosocomial infections and a leading cause of patient mortality. Pseudomonas aeruginosa infections are frequently associated with the development of biofilms, which give the bacteria additional drug resistance and increase their virulence. The goal of this study was to find strong compounds that block the Anthranilate-CoA ligase enzyme made by the pqsA gene. This would stop the P. aeruginosa quorum signaling system. This enzyme plays a crucial role in the pathogenicity of P. aeruginosa by producing autoinducers for cell-to-cell communication that lead to the production of biofilms. Pharmacophore-based virtual screening was carried out utilizing a library of commercially accessible enzyme inhibitors. The most promising hits obtained during virtual screening were put through molecular docking with the help of MOE. The virtual screening yielded 7/160 and 10/249 hits (ZINC and Chembridge). Finally, 2/7 ZINC hits and 2/10 ChemBridge hits were selected as potent lead compounds employing diverse scaffolds due to their high pqsA enzyme binding affinity. The results of the pharmacophore-based virtual screening were subsequently verified using a molecular dynamic simulation-based study (MDS). Using MDS and post-MDS, the stability of the complexes was evaluated. The most promising lead compounds exhibited a high binding affinity towards protein-binding pocket and interacted with the catalytic dyad. At least one of the scaffolds selected will possibly prove useful for future research. However, further scientific confirmation in the form of preclinical and clinical research is required before implementation.
Effectiveness and Safety of Belimumab in Chinese Lupus Patients: A Multicenter, Real-World Observational Study
Authors: Sun Fangfang , Wu Huaxiang , Wang Zitao , Wu Tong , Wu Xue , Chen Jie , Zhang Danting , Bao Chunde , Shen Nan , Wu Lijun , Zhu Jing , Ye Shuang
Published: March 2023

Objective: The effectiveness and safety of belimumab in Chinese lupus patients with different disease activities were investigated in a real-world setting. Method: Patients who received 10 mg/kg belimumab intravenously on weeks 0, 2, and 4, and then every 4 weeks on a background of standard-of-care (SoC) therapy and had a follow-up of more than 6 months were enrolled from four centers in China. They were stratified according to the Safety of Estrogens in Lupus Erythematosus National Assessment-SLE Disease Activity Index (SELENA-SLEDAI) score at baseline as the moderate/severe (SELENA-SLEDAI > 6) or mild subgroups (SELENA-SLEDAI ≤ 6). Attainment of the Lupus Low Disease Activity State (LLDAS) or remission on treatment was analyzed in all patients. The SLE Responder Index 4 (SRI-4) and SELENA-SLEDAI Flare Index (SFI) were evaluated for patients with moderate/severe disease and mild disease, respectively. Patients in the control arm with SoC alone from previous metformin lupus trials were selected by propensity score matching (PSM) as the reference group. Results: 224 SLE patients with a mean follow-up of 11.7 months receiving belimumab were enrolled in this observational study, of which 126 and 98 were in the moderate/severe and mild subgroup, respectively. At 12 months, 54.76% of the patients attained LLDAS and 28.57% attained remission. Lower daily prednisone at baseline were independently associated with 12-month LLDAS. Further, 87% of the subgroup with moderate/severe disease achieved SRI-4 at 12 months and a high SLEDAI at baseline was its predictive factor. For the mild subgroup, a reduced flare rate was observed compared with PSM reference (17.5%, vs. 38.6%, p = 0.021). Infection events, particularly viral infections and pneumonia were recorded in 7 and 6 patients, respectively. Conclusion: Our real-world data supported the effectiveness and safety of belimumab in Chinese lupus patients.
Mechanical Characterization of 3D-Printed Patterned Membranes for Cardiac Tissue Engineering: An Experimental and Numerical Study
Authors: Poerio Aurelia , Guibert Bertrand , Leroux Mélanie M. , Mano João F. , Cleymand Franck , Jehl Jean-Philippe
Published: March 2023

A myocardial infarction can cause irreversible damage to the heart muscle. A promising approach for the treatment of myocardial infarction and prevention of severe complications is the application of cardiac patches or epicardial restraint devices. The challenge for the fabrication of cardiac patches is the replication of the fibrillar structure of the myocardium, in particular its anisotropy and local elasticity. In this study, we developed a chitosan–gelatin–guar gum-based biomaterial ink that was fabricated using 3D printing to create patterned anisotropic membranes. The experimental results were then used to develop a numerical model able to predict the elastic properties of additional geometries with tunable elasticity that could easily match the mechanical properties of the heart tissue (particularly the myocardium).
Circulating miRNA as a Biomarker in Oral Cancer Liquid Biopsy
Authors: Roi Alexandra , Boia Simina , Rusu Laura-Cristina , Roi Ciprian Ioan , Boia Eugen Radu , Riviș Mircea
Published: March 2023

Oral cancer is currently challenging the healthcare system, with a high incidence among the population and a poor survival rate. One of the main focuses related to this malignancy is the urge to implement a viable approach for improving its early diagnosis. By introducing the use of liquid biopsy and the identification of potential biomarkers, aiming for a noninvasive approach, new advancements offer promising perspectives in the diagnosis of oral cancer. The present review discusses the potential of circulating miRNAs as oral cancer biomarkers identified in body fluids such as serum, plasma, and saliva samples of oral cancer patients. Existing results reveal an important implication of different miRNA expressions involved in the initiation, development, progression, and metastasis rate of oral malignancy. Liquid biomarkers can play a crucial role in the development of the concept of personalized medicine, providing a wide range of clinical applications and future targeted therapies.
Recent Biomedical Applications of Coupling Nanocomposite Polymeric Materials Reinforced with Variable Carbon Nanofillers
Authors: Alosaimi Abeer M. , Alorabi Randa O. , Katowah Dina F. , Al-Thagafi Zahrah T. , Alsolami Eman S. , Hussein Mahmoud A. , Qutob Mohammad , Rafatullah Mohd
Published: March 2023

The hybridization between polymers and carbon materials is one of the most recent and crucial study areas which abstracted more concern from scientists in the past few years. Polymers could be classified into two classes according to the source materials synthetic and natural. Synthetic polymeric materials have been applied over a floppy zone of industrial fields including the field of biomedicine. Carbon nanomaterials including (fullerene, carbon nanotubes, and graphene) classified as one of the most significant sources of hybrid materials. Nanocarbons are improving significantly mechanical properties of polymers in nanocomposites in addition to physical and chemical properties of the new materials. In all varieties of proposed bio-nanocomposites, a considerable improvement in the microbiological performance of the materials has been explored. Various polymeric materials and carbon-course nanofillers were present, along with antibacterial, antifungal, and anticancer products. This review spots the light on the types of synthetic polymers-based carbon materials and presented state-of-art examples on their application in the area of biomedicine.
Updates on Lymphovascular Invasion in Breast Cancer
Authors: Kuhn Elisabetta , Gambini Donatella , Despini Luca , Asnaghi Dario , Runza Letterio , Ferrero Stefano
Published: March 2023

Traditionally, lymphovascular invasion (LVI) has represented one of the foremost pathological features of malignancy and has been associated with a worse prognosis in different cancers, including breast carcinoma. According to the most updated reporting protocols, the assessment of LVI is required in the pathology report of breast cancer surgical specimens. Importantly, strict histological criteria should be followed for LVI assessment, which nevertheless is encumbered by inconsistency in interpretation among pathologists, leading to significant interobserver variability and scarce reproducibility. Current guidelines for breast cancer indicate biological factors as the main determinants of oncological and radiation therapy, together with TNM staging and age. In clinical practice, the widespread use of genomic assays as a decision-making tool for hormone receptor-positive, HER2-negative breast cancer and the subsequent availability of a reliable prognostic predictor have likely scaled back interest in LVI’s predictive value. However, in selected cases, the presence of LVI impacts adjuvant therapy. This review summarizes current knowledge on LVI in breast cancer with regard to definition, histopathological assessment, its biological understanding, clinicopathological association, and therapeutic implications.
G-Quadruplexes in c-MYC Promoter as Targets for Cancer Therapy
Authors: Bahls Bárbara , Aljnadi Israa M. , Emídio Rita , Mendes Eduarda , Paulo Alexandra
Published: March 2023

Cancer is a societal burden demanding innovative approaches. A major problem with the conventional chemotherapeutic agents is their strong toxicity and other side effects due to their poor selectivity. Uncontrolled proliferation of cancer cells is due to mutations, deletions, or amplifications in genes (oncogenes) encoding for proteins that regulate cell growth and division, such as transcription factors, for example, c-MYC. The direct targeting of the c-MYC protein has been attempted but so far unsuccessfully, as it lacks a definite binding site for the modulators. Meanwhile, another approach has been explored since the discovery that G-quadruplex secondary DNA structures formed in the guanine-rich sequences of the c-MYC promoter region can downregulate the transcription of this oncogene. Here, we will overview the major achievements made in the last decades towards the discovery of a new class of anticancer drugs targeting G-quadruplexes in the c-MYC promoter of cancer cells.
Insight on Infections in Diabetic Setting
Authors: Pari Bianca , Gallucci Matteo , Ghigo Alberto , Brizzi Maria Felice
Published: March 2023

The correlation between diabetes mellitus and infectious diseases is widely recognized. DM patients are characterized by the impaired function of the immune system. This translates into the occurrence of a variety of infections, including urinary tract, skin and surgical site infections, pneumonia, tuberculosis, and, more recently, SARS-CoV-2. Hyperglycemia has been identified as a relevant factor contributing to unfavorable outcomes in hospitalized patients including SARS-CoV-2 patients. Several studies have been performed proving that to maintain the proper and stringent monitoring of glycemia, a balanced diet and physical activity is mandatory to reduce the risk of infections and their associated complications. This review is focused on the mechanisms accounting for the increased susceptibility of DM patients to infections, with particular attention to the impact of newly introduced hypoglycemic drugs in sepsis management.
The Effects of Using Virtual Reality on Thai Word Order Learning
Authors: Watthanapas Nitiwat , Hao Yung-Wei , Ye Jian-Hong , Hong Jon-Chao , Ye Jhen-Ni
Published: March 2023

Thai has its own unique spelling system and grammatical rules. Its word order is quite different from that of Mandarin and English, thus making it more difficult for students in Taiwan to learn. Past studies also point out that learning word order is one of the most difficult aspects when learning foreign languages. As science and technology advance, emerging technologies have been widely applied in foreign language learning. This research aims to explore the effect of using a multi-language VR learning assessment system on assisting Thai learners to learn grammatical word order, and to investigate the correlates between Thai self-efficacy, Thai language anxiety, word order learning retention, and task value of VR learning. In order to accomplish this purpose, we invited Thai learners who took Thai courses in the continuing education division of a national university in northern Taiwan to participate in a 5-week teaching experiment, during which the participants were asked to practice Thai word order for 20 min. They were administered a questionnaire to fill out after five weeks of practice and were tested for retention one month after the experiment. A total of 84 valid questionnaires were collected, with an effective return rate of 93.3%. Of the respondents, 30 were male (35.7%), and 54 were female (64.3%). The data were subjected to item analysis, reliability and validity analysis, and then underwent PLS-SEM for research model validation. The results revealed that: (1) Thai language self-efficacy was positively related to learning retention and task value; (2) Thai language anxiety was negatively related to learning retention and task value; (3) Learning retention was positively related to the task value of learning and continuous usage intention.
The Relationship between Teachers and Pupils with Down Syndrome: A Qualitative Study in Primary Schools
Authors: Carbone Agostino , Castaldi Michela , Szpunar Giordana
Published: March 2023

Down syndrome (DS), due the presence of an intellectual disability and a precarious health condition, involves important challenges for developing skills at school. The study explores the relational dynamics between teachers and students with DS and how the latter influence the processes of socialization and learning at school. The study involved 15 (n = 15) special education teachers (M age = 40.4; SD = 9.3) from primary schools in Italy who were or had previously been in charge of a pupil with DS. The teachers were interviewed through a semi-structured interview, built ad hoc. The data were analyzed through the grounded theory method supported by ATLAS.ti software. Data analysis produced 20 (n = 20) categories grouped into 6 (n = 6) macro-categories: (1) psychophysical characteristics; (2) learnings; (3) relational aspects; (4) collaborations; (5) extracurricular activities; and (6) teacher training. The research shows that students with DS have good relationships with their peers; however, there are still some important critical issues, including poor training of teachers on certain topics and on the application of collaborative and relationship support strategies, the limited collaboration between support teachers and curricular teachers, and the absence of support from ad hoc professional figures.
Bioactive Glass Inhibits Tumor Development from Giant Cell Tumor of Bone-Derived Neoplastic Stromal Cells in a Chicken Chorioallantoic Membrane Assay
Authors: Fellenberg Joerg , Losch Sarina , Marinescu Max R. , Frey Birgit , Lehner Burkhard , Arango-Ospina Marcela , Hadzhieva Zoya , Boccaccini Aldo R. , Westhauser Fabian
Published: March 2023

Tumor recurrence is a major problem during the treatment of giant cell tumors of bone (GCTB). We recently identified tumor cell-specific cytotoxic effects of bioactive glasses (BGs) toward neoplastic stromal cells derived from GCTB tissue (GCTSCs) in vitro. Since these data indicated a promising role of BGs in the adjuvant treatment of GCTBs, we aimed to investigate the transferability of the in vitro data into the more complex in vivo situation in the current study. We first analyzed the cytotoxicity of three different BGs in vitro by WST-1 assay after co-cultivation with primary GCTSC cell lines. The effects of BGs on tumor engraftment and growth were analyzed by chicken chorioallantoic membrane (CAM) assays and subsequent quantification of tumor take rates and tumor volumes. In vitro, all tested BGs displayed a cytotoxic effect on GCTSCs that was dependent on BG composition, concentration, and particle size. Comparable effects could be observed within the in vivo environment resulting in reduced tumor take rates and tumor volumes in BG-treated samples. These data indicate a possible clinical application of BGs in the context of GCTB therapy, mediating a reduction of recurrence rates with the simultaneous promotion of bone regeneration.
Investigation of 11p15.5 Methylation Defects Associated with Beckwith-Wiedemann Spectrum and Embryonic Tumor Risk in Lateralized Overgrowth Patients
Authors: Tüysüz Beyhan , Bozlak Serdar , Uludağ Alkaya Dilek , Ocak Süheyla , Kasap Büşra , Sunamak Çifçi Evrim , Seker Ali , Bayhan Ilhan Avni , Apak Hilmi
Published: March 2023

The Beckwith–Wiedemann spectrum (BWSp) ranges from isolated lateralized overgrowth (ILO) to classic phenotypes. In this broad clinical spectrum, an epigenetic alteration on chromosome 11p15.5 can be detected. The risk for embryonal tumors is high, especially in patients with lateralized overgrowth (LO). The aim of this study is to investigate epigenetic alterations in 11p15.5 and tumor risk in 87 children with LO. The methylation level of 11p15.5 was examined in the blood of all patients and in skin samples or buccal swabs from 40 patients with negative blood tests; 63.2% of patients were compatible with the ILO phenotype, 18.4% were atypical, and 18.4% were classic. The molecular diagnosis rate was 81.2% for the atypical and classic phenotypes, and 10.9% for the ILO phenotype. In patients with epigenetic alterations, LO was statistically significantly more severe than in test negatives. Tumors developed in six (6.9%) of the total 87 patients with LO; four belonged to the atypical or classical phenotype (12.5%) and two to ILO (3.5%). Three of the four patients with atypical/classical phenotypes had pUPD11, one had IC1-GOM alteration, and two ILO patients were negative. We conclude that LO patients should be monitored for tumor risk even if their epigenetic tests are negative.
Cellular Mechanisms of Skin Diseases
Authors: Goldust Mohamad
Published: March 2023

Skin plays an important role in protecting and enhancing health [...]
Correction: Furlan, G. and Galupa R. Mechanisms of Choice in X-Chromosome Inactivation. Cells 2022, 11, 535
Authors: Furlan Giulia , Galupa Rafael
Published: March 2023

The authors wish to make the following changes to their paper [...]
Human Stem Cell Use in Androgenetic Alopecia: A Systematic Review
Authors: Krefft-Trzciniecka Katarzyna , Piętowska Zuzanna , Nowicka Danuta , Szepietowski Jacek C.
Published: March 2023

Androgenetic alopecia is a condition that results in hair loss in both men and women. This can have a significant impact on a person’s psychological well-being, which can lead to a decreased quality of life. We conducted a systematic review to evaluate the efficacy of using stem cells in androgenic alopecia. The search was conducted in MEDLINE via PubMed, Web of Science, and Scopus databases. The review was performed on data pertaining to the efficacy of using different types of stem cells in androgenic alopecia: quantitative results of stem cell usage were compared to the control treatment or, different types of treatment for female and male androgenetic alopecia. Of the outcomes, the density of hair was analyzed. Fourteen articles were selected for this review. During and after treatment with stem cells, no major side effects were reported by patients with alopecia. The use of stem cells in androgenic alopecia seems to be a promising alternative to the standard treatment or it could play the role of complementary therapy to improve the effect of primary treatment. However, these results should be interpreted with caution until they can be reproduced in larger and more representative samples.
Targeting ARID1A-Deficient Cancers: An Immune-Metabolic Perspective
Authors: Lebedev Timofey , Kousar Rubina , Patrick Bbumba , Usama Muhammad , Lee Meng-Kuei , Tan Ming , Li Xing-Guo
Published: March 2023

Epigenetic remodeling and metabolic reprogramming, two well-known cancer hallmarks, are highly intertwined. In addition to their abilities to confer cancer cell growth advantage, these alterations play a critical role in dynamically shaping the tumor microenvironment and antitumor immunity. Recent studies point toward the interplay between epigenetic regulation and metabolic rewiring as a potentially targetable Achilles’ heel in cancer. In this review, we explore the key metabolic mechanisms that underpin the immunomodulatory role of AT-rich interaction domain 1A (ARID1A), the most frequently mutated epigenetic regulator across human cancers. We will summarize the recent advances in targeting ARID1A-deficient cancers by harnessing immune-metabolic vulnerability elicited by ARID1A deficiency to stimulate antitumor immune response, and ultimately, to improve patient outcome.
The Application of Combined Visible and Ultraviolet Irradiation to Improve the Functional Characteristics of Gas Sensors Based on ZnO/SnO2 and ZnO/Au Nanorods
Authors: Ivanishcheva Alexandra P. , Sysoev Victor V. , Abdullin Khabibulla A. , Nesterenko Andrey V. , Khubezhov Soslan A. , Petrov Victor V.
Published: March 2023

Arrays of zinc oxide (ZnO) nanorods were synthesized over quartz substrates by the hydrothermal method. These nanorods were grown in a predominantly vertical orientation with lengths of 500–800 nm and an average cross-sectional size of 40–80 nm. Gold, with average sizes of 9 ± 1 nm and 4 ± 0.5 nm, and tin nanoclusters, with average sizes of 30 ± 5 nm and 15 ± 3 nm, were formed on top of the ZnO nanorods. Annealing was carried out at 300 °C for 2 h to form ZnO/SnO2 and ZnO/Au nanorods. The resulting nanorod-arrayed films were comprehensively studied using X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectrometry (EDS) and X-ray photoelectron spectroscopy (XPS). To fabricate resistive sensor elements, the films were supplied with V/Ni contact metallization on top of the nanorods. The gas sensor performance of the prepared films was evaluated at various temperatures in order to select 200 °C as the optimum one which enabled a selective detection of NO2. Adding UV-viz irradiation via a light-emitting diode, λ = 400 nm, allowed us to reduce the working temperature to 50 °C and to advance the detection limit of NO2 to 0.5 ppm. The minimum response time of the samples was 92 s, which is 9 times faster than in studies without exposure to UV-viz radiation.
Risk Factors for Persistent Anosmia and Dysgeusia in Children with SARS-CoV-2 Infection: A Retrospective Study
Authors: Mariani Francesco , Morello Rosa , Traini Daniele Omar , La Rocca Anna , De Rose Cristina , Valentini Piero , Buonsenso Danilo
Published: March 2023

Background: Olfactory and gustative dysfunctions are two of the most common post-acute sequelae of SARS-CoV-2 infection in children, which can have a negative impact on the routines of children and families. As several children have had COVID-19 since the Omicron variant, it is important to investigate if this increase in infections is reflected in higher olfactory/taste disfunctions. The primary aim of this study was to characterize the presence of olfactory/gustative problems in a cohort of children, its evolution, and its association with risk factors such as COVID-19 variant, hospitalization, presence of olfactory/gustative dysfunction during the acute phase, and vaccination. Methods: This was a retrospective analysis of children with microbiologically confirmed SARS-CoV-2 infection evaluated in person at a referral pediatric post-COVID-19 clinic in Rome, Italy. We included children younger than 19 years old, evaluated from the beginning of the pandemic up to October 2022. At specific timepoints, we investigated the presence of olfactory/taste disfunctions and evaluated them according to the SARS-CoV-2 variants circulating at the time of infection. Results: A total of 1250 children (650 females; 52.0%) with a mean age of 6.77 (±4.12) years were included in the study. At 3, 6, 12, and 18 months, 12 (9.6%), 7 (5.6%), 2 (1.6%), and 1 (0.8%) of the children reported anosmia and dysgeusia post-COVID-19 infection, respectively. The presence of anosmia and dysgeusia during the acute phase of infection and being infected with a pre-Omicron variant were found to be significant risk factors for persistent olfactory and gustatory dysfunction during all follow-up periods. Conclusions: anosmia and dysgeusia symptoms tended to decrease gradually over time, but not all children recovered quickly.
Alteration of Mesenchymal Stem Cells Isolated from Glioblastoma Multiforme under the Influence of Photodynamic Treatment
Authors: Tumangelova-Yuzeir Kalina , Minkin Krassimir , Angelov Ivan , Ivanova-Todorova Ekaterina , Kurteva Ekaterina , Vasilev Georgi , Arabadjiev Jeliazko , Karazapryanov Petar , Gabrovski Kaloyan , Zaharieva Lidia , Genova Tsanislava , Kyurkchiev Dobroslav
Published: March 2023

The central hypothesis for the development of glioblastoma multiforme (GBM) postulates that the tumor begins its development by transforming neural stem cells into cancer stem cells (CSC). Recently, it has become clear that another kind of stem cell, the mesenchymal stem cell (MSC), plays a role in the tumor stroma. Mesenchymal stem cells, along with their typical markers, can express neural markers and are capable of neural transdifferentiation. From this perspective, it is hypothesized that MSCs can give rise to CSC. In addition, MSCs suppress the immune cells through direct contact and secretory factors. Photodynamic therapy aims to selectively accumulate a photosensitizer in neoplastic cells, forming reactive oxygen species (ROS) upon irradiation, initiating death pathways. In our experiments, MSCs from 15 glioblastomas (GB-MSC) were isolated and cultured. The cells were treated with 5-ALA and irradiated. Flow cytometry and ELISA were used to detect the marker expression and soluble-factor secretion. The MSCs’ neural markers, Nestin, Sox2, and glial fibrillary acid protein (GFAP), were down-regulated, but the expression levels of the mesenchymal markers CD73, CD90, and CD105 were retained. The GB-MSCs also reduced their expression of PD-L1 and increased their secretion of PGE2. Our results give us grounds to speculate that the photodynamic impact on GB-MSCs reduces their capacity for neural transdifferentiation.
Coating Technology Makes Comprehensive Surface Protection Possible
Authors: Ma Chi
Published: March 2023

The tribology and wear protection of coatings has been an active and rapidly developing area for research and industries in recent years, with innovative coating materials, structures, and fabrication technologies [...]
Investigating the Individual Performances of Coupled Superconducting Transmon Qubits
Authors: Ahmad Halima Giovanna , Jordan Caleb , van den Boogaart Roald , Waardenburg Daan , Zachariadis Christos , Mastrovito Pasquale , Georgiev Asen Lyubenov , Montemurro Domenico , Pepe Giovanni Piero , Arthers Marten , Bruno Alessandro , Tafuri Francesco , Mukhanov Oleg , Arzeo Marco , Massarotti Davide
Published: March 2023

The strong requirement for high-performing quantum computing led to intensive research on novel quantum platforms in the last decades. The circuital nature of Josephson-based quantum superconducting systems powerfully supports massive circuital freedom, which allowed for the implementation of a wide range of qubit designs, and an easy interface with the quantum processing unit. However, this unavoidably introduces a coupling with the environment, and thus to extra decoherence sources. Moreover, at the time of writing, control and readout protocols mainly use analogue microwave electronics, which limit the otherwise reasonable scalability in superconducting quantum circuits. Within the future perspective to improve scalability by integrating novel control energy-efficient superconducting electronics at the quantum stage in a multi-chip module, we report on an all-microwave characterization of a planar two-transmon qubits device, which involves state-of-the-art control pulses optimization. We demonstrate that the single-qubit average gate fidelity is mainly limited by the gate pulse duration and the quality of the optimization, and thus does not preclude the integration in novel hybrid quantum-classical superconducting devices.
Numerical and Experimental Analysis of the ZFC Heat Release from a YBCO Bulk and Validation of YBCO Thermal Parameters
Authors: Arsénio Costa António J. , Fernandes João F. P. , Melicio Rui , Cardeira Carlos , Costa Branco Paulo J.
Published: March 2023

This article presents results from a simple experimental methodology used to determine the amount of heat transferred from an yttrium barium copper oxide (YBCO) bulk to liquid nitrogen (LN2) and LN2 consumption during the process of zero-field cooling (ZFC). The thermal power can be determined from the YBCO bulk temperature variation, which is difficult to measure with accuracy. In this procedure, the thermal power from the YBCO bulk to LN2 is determined from the measured rate of LN2 evaporation, considering the LN2 latent heat. To reduce the influence of room temperature heating and make the LN2 mass variation depend as much as possible on the heat released from the YBCO bulk, a step transient from room temperature into the LN2 is performed. The precision of results is determined from the rate of LN2 evaporation due to room temperature heating with the bulk already cooled by ZFC. The temperature evolution at the bulk lateral surface where the heat transfer is higher is also measured. The results from experimental measurements are compared with 3D finite element analysis (FEA) numerical results. The obtained evolutions of the temperature and thermal power from the YBCO bulk are used to validate YBCO thermal parameters, such as thermal conductivity and specific heat capacity at constant pressure. The YBCO bulk equivalent heat capacity and thermal resistance are determined by analyzing the equivalent first-order thermal lumped parameter circuit based on the obtained evolutions in time of the YBCO temperature and heat transferred to the LN2. The characteristics of dependence of the YBCO thermal resistance and heat capacity with temperature are obtained by correlating their time evolutions with the bulk average temperature evolution in time. The YBCO-specific heat capacity at constant pressure is then calculated by dividing the obtained bulk heat capacity by the bulk mass. The YBCO thermal conductivity is calculated from the obtained thermal resistance considering an equivalent bulk section and length toward the main direction of heat flux.
Thermoelectrical Properties of ITO/Pt, In2O3/Pt and ITO/In2O3 Thermocouples Prepared with Magnetron Sputtering
Authors: Liu Yantao , Shi Peng , Ren Wei , Huang Rong
Published: March 2023

ITO/Pt, In2O3/Pt and ITO/In2O3 thermocouples were prepared by the radio frequency (RF) magnetron sputtering method. The XRD results showed that all the annealed ITO and In2O3 films annealed at high temperature present a cubic structure. Scanning electron microscope results showed that the thickness of the ITO and In2O3 films could reach 1.25 µm and 1.21 µm, respectively. The ITO/Pt and In2O3/Pt thin film thermocouples could obtain an output voltage of 68.7 mV and 183.5 mV, respectively, under a 900 °C temperature difference, and at the same time, the Seebeck coefficient reached 76.1 µV/°C and 203.9 µV/°C, respectively. For the ITO/In2O3 thermocouple, the maximum value of the output voltage was 165.7 mV under a 1200 °C temperature difference, and the Seebeck coefficient was 138.1 µV/°C. Annealing under different atmosphere conditions under 1000 °C, including vacuum, air and nitrogen atmospheres, resulted in values of the Seebeck coefficient that were 138.2 µV/°C, 135.5 µV/°C and 115.7 µV/°C, respectively.
Self-Assembly Heterometallic Cu-Ln Complexes: Synthesis, Crystal Structures and Magnetic Characterization
Authors: Zhang Shaoliang , Du Ruili , Fan Xiufang , Zhao Xinhua , Wang Yanlan , Li Shanshan
Published: March 2023

Using N2O4 donor symmetric ligand H2L and dca co-ligand, two new isostructural dinuclear CuII–LnIII complexes [Cu(Cl)(L)Ln(NO3)(CH3OH)(H2O)(dca)] [Ln=Ho (1CuHo), Gd (2CuGd)] [H2L = 6,6′-((1E,1′E)-(ethane-1,2-diylbis(azaneylylidene))bis(methaneylylidene))bis(2-methoxyphenol); dca=dicyanamide] were designed, synthesized and studied. In the two isostructural compounds, the geometric environment around the nine-coordinated Ln(III) ions is muffin, whereas the geometry of the penta-coordinated Cu(II) ions is square pyramid. The magnetic properties of both complexes were also studied. Direct current magnetic susceptibility measurements indicate ferromagnetic interactions between the Cu(II) ion and Gd(III) ion in complex 2CuGd. Alternating current (ac) magnetic measurements indicate that complex 1CuHo displays slow magnetic relaxation behaviour.
The Synthesis and Domain Structures of Single-Crystal-Like Mesoscale BaTiO3 Plates
Authors: Zheng Kun , Zhuang Jian , Quan Yi , Zhao Jinyan , Wang Lingyan , Wang Zhe , Ren Wei
Published: March 2023

The (001) plate-like BaTiO3 piezoelectric micromaterials are synthesized by topochemical microcrystal conversion technique. BaTiO3 plates with a length of 2~10 μm and thickness of 0.5~1.3 μm are obtained. The dependence of morphology on synthesis conditions is discussed in detail. The crystal symmetry and multiscale domain structures of BaTiO3 plates are systematically investigated by various characterizations. X-ray diffraction (XRD) and Raman spectra analyses demonstrate the tetragonal symmetry of the (001) oriented BaTiO3 plates at room temperature. The domain configurations of the micron BaTiO3 are investigated with a polarized light microscope (PLM) and piezoresponse force microscopy (PFM). The single-crystal-like quality and uniformity are supported by PLM observations. More importantly, the classical 90° banded ferroelectric domains of ~125 nm width are observed for the first time in such BaTiO3 plates. The domain features in the mesoscale BaTiO3 plate are discussed and compared with its bulk counterparts. The results may provide insights into understanding and designing the mesoscale BaTiO3 functional materials.
Artificial Neural Network for the Prediction of Fatigue Life of Microscale Single-Crystal Copper
Authors: Zeng Fanming , Yan Yabin
Published: March 2023

Microscale single-crystal copper is widely used in electronics, communications and other fields due to its excellent properties such as high ductility, high toughness and good conductivity. Therefore, it is particularly important to research its fatigue life. In order to explore the influence of size effect, loading frequency and shear strain on the main slip surface on the fatigue life of microscale single-crystal copper based on in situ fatigue experimental data of microscale single-crystal copper, this paper used a BP neural network algorithm to construct a single-crystal copper fatigue life prediction network model. The data set included 14 groups of training data, with 11 groups as training sets and 3 groups as testing sets. The input characteristics were length, width, height, loading frequency and shear strain of the main sliding plane of a microscale single-crystal copper sample. The output characteristic was the fatigue life of microscale single-crystal copper. After training, the mean square error (MSE) of the model was 0.03, the absolute value error (MAE) was 0.125, and the correlation coefficient (R2) was 0.93271, indicating that the BP neural network algorithm can effectively predict the fatigue life of microscale single-crystal copper and has good generalization ability. This model can not only save the experimental time of fatigue life measurement of micro-scale single-crystal copper, but also optimize the properties of the material by taking equidistant points in the range of characteristic parameters. Therefore, the current study demonstrates an applicable and efficient methodology to evaluate the fatigue life of microscale materials in industrial applications.
A Review of Research on the Mustard Hill Coral, Porites astreoides
Authors: Eagleson Ryan G. , Álvarez-Filip Lorenzo , Lumsden John S.
Published: March 2023

Coral reefs are the most diverse habitat per unit area in the world’s oceans, supporting an estimated 1–3 million species in only 0.2% of its area. These ecosystems have suffered severe declines since the 1970s, largely as a result of climate change, ocean acidification, pollution, disease, and overfishing. Porites astreoides is a shallow species that is able to thrive in a variety of environmental conditions and has been a clear ‘winner’ on Atlantic reefs in the last decades. This, coupled with its ease of identification and wide distribution, has caused P. astreoides to become a focal species in many scientific studies. Given the current and increasing significance of P. astreoides, this review sought to (i) identify the key life history traits that allowed this species to thrive under stressful conditions; (ii) compile aspects of its biology and ecology to understand its future contribution to Atlantic reefs, and (iii) identify knowledge gaps. To date, no comprehensive overview of the literature exists for P. astreoides. All articles available on Google Scholar up to the time of submission containing the terms ‘Mustard Hill Coral’, ‘Porites astreoides’, or ‘P. astreoides’ were examined for potential inclusion in this review. Papers were assessed based on whether they captured the most influential or widespread theories, represented an important trend in the research, or contained novel findings relevant to the understanding of this species. This review provides a scholarly resource and wide-ranging synthesis of P. astreoides on Atlantic reefs of today and the future.
Editorial for Special Issue “Advance in Diagnostic and Management of Ischemic Heart Disease”
Authors: Dorobanțu Maria
Published: March 2023

Ischemic heart disease is one of the leading causes of morbidity and mortality worldwide [...]
The Accuracy of Pre-Endoscopic Scores for Mortality Prediction in Patients with Upper GI Bleeding and No Endoscopy Performed
Authors: Cazacu Sergiu Marian , Alexandru Dragoș Ovidiu , Statie Răzvan-Cristian , Iordache Sevastița , Ungureanu Bogdan Silviu , Iovănescu Vlad Florin , Popa Petrică , Sacerdoțianu Victor Mihai , Neagoe Carmen Daniela , Florescu Mirela Marinela
Published: March 2023

(1) Background: The assessment of mortality and rebleeding rate in upper gastrointestinal bleeding (UGIB) is essential, and several prognostic scores have been proposed. Some patients with UGIB did not undergo endoscopy, either because they refused the procedure, suffered from alcohol withdrawal symptoms or altered general status, or because the bleeding was severe enough to cause death before the endoscopy. The mortality risk in the subgroup of patients without endoscopy is poorly evaluated in the literature. (2) Methods: The purpose of the study was to identify the most useful scores for the assessment of in-hospital mortality in patients with UGIB with no endoscopy performed and no known etiology. A total of 198 patients with UGIB and no endoscopy performed were admitted between January 2017 and December 2021 and the accuracy of 12 prognostic scores and the Charlson comorbidity index for in-hospital mortality prediction were analyzed, as well as Child–Pugh Turcotte (CPT) and Meld scores in patients with cirrhosis. (3) Results: The mortality rate was 37.9%, higher than in variceal (21.9%, p < 0.0001) and non-variceal bleeding (7.4%, p < 0.0001). The most accurate scores by AUC were the International Bleeding score (INBS, 0.844), Glasgow Blatchford (0.783), MAP score (0.78), Iino (0.766), AIM65 and modified N-score (0.745 each), modified Glasgow-Blatchford (0.73), H3B2 and N-score (0.701); Rockall, Baylor, and T-score had an AUC below 0.7. MELD score was superior to CPT in patients with cirrhosis (AUC 0.811 versus 0.670). (4) Conclusions: The mortality rate in UGIB with no endoscopy was higher than in both variceal and non-variceal bleeding and was higher in the pandemic period but with no statistical significance (45.3% versus 32.14%, p = 0.0586), mainly because of positive cases. Only one case of rebleeding was noted; the hospitalization period was significantly shorter. The most accurate score was International Bleeding Score; the MELD score had a higher but moderate accuracy compared with CPT in patients with cirrhosis.
Quantization of Integrable and Chaotic Three-Particle Fermi–Pasta–Ulam–Tsingou Models
Authors: Arzika Alio Issoufou , Solfanelli Andrea , Schmid Harald , Ruffo Stefano
Published: March 2023

We study the transition from integrability to chaos for the three-particle Fermi–Pasta–Ulam–Tsingou (FPUT) model. We can show that both the quartic β-FPUT model (α=0) and the cubic one (β=0) are integrable by introducing an appropriate Fourier representation to express the nonlinear terms of the Hamiltonian. For generic values of α and β, the model is non-integrable and displays a mixed phase space with both chaotic and regular trajectories. In the classical case, chaos is diagnosed by the investigation of Poincaré sections. In the quantum case, the level spacing statistics in the energy basis belongs to the Gaussian orthogonal ensemble in the chaotic regime, and crosses over to Poissonian behavior in the quasi-integrable low-energy limit. In the chaotic part of the spectrum, two generic observables obey the eigenstate thermalization hypothesis.
More Stages Decrease Dissipation in Irreversible Step Processes
Authors: Salamon Peter , Andresen Bjarne , Nulton James , Roach Ty N. F. , Rohwer Forest
Published: March 2023

The dissipation in an irreversible step process is reduced when the number of steps is increased in any refinement of the steps in the process. This is a consequence of the ladder theorem, which states that, for any irreversible process proceeding by a sequence of relaxations, dividing any relaxation step into two will result in a new sequence that is more efficient than the original one. This results in a more-steps-the-better rule, even when the new sequence of steps is not reoptimized. This superiority of many steps is well established empirically in, e.g., insulation and separation applications. In particular, the fact that the division of any step into two steps improves the overall efficiency has interesting implications for biological evolution and emphasizes thermodynamic length as a central measure for dissipation.
The Effect of Ice on Shoulder Proprioception in Badminton Athletes
Authors: Marouvo Joel , Tavares Nuno , Dias Gonçalo , Castro Maria António
Published: March 2023

This study aims to analyze the influence of the application of cryotherapy on shoulder proprioception in badminton athletes. Thirty federated badminton athletes were included in this study, all of whom belonged to three of the teams currently competing in national competitions (Portugal). Their mean ages were 21.00 ± 5.60 years, and their experience in the modality was 8.40 ± 6.93 years. They practiced in an average of 2.93 ± 1.26 training sessions per week. All of them used their right hand to hold the racket. Each participant’s dominant shoulder joint position and force senses were evaluated for four consecutive time points through the isokinetic dynamometer Biodex System 3. The experimental procedure consisted of applying ice for 15 min and the control procedure consisted of no therapeutic intervention. The proprioception outcomes were expressed using the absolute error, relative error, and variable error. All statistical analysis was performed using PASW Statistics 18 software (IBM-SPSS Statistics). There were no statistically significant changes in the joint position and force senses after the intervention, as well as during the subsequent 30 min. We conclude that, after this cryotherapy technique, there is no increased risk of injury associated with a proprioception deficit that prevents athletes from immediately returning to badminton practice.
Soft-Sensor Modeling of Temperature Variation in a Room under Cooling Conditions
Authors: Xu Feng , Sakurai Kei , Sato Yuki , Sakai Yuka , Sabu Shunsuke , Kanayama Hiroaki , Satou Daisuke , Kansha Yasuki
Published: March 2023

Non-uniform temperature distributions in air-conditioned areas can reduce the energy efficiency of air conditioners and cause uncomfortable thermal sensations for occupants. Furthermore, it is impractical to use physical sensors to measure the local temperature at every position. This study developed a soft-sensing model that integrates the fundamentals of thermodynamics and transport phenomena to predict the temperature at the target position in space. Water experiments were conducted to simulate indoor conditions in an air-conditioning cooling mode. The transient temperatures of various positions were measured for model training and validation. The velocity vectors of water flow were acquired using the particle image velocimetry method. Correlation analysis of various positions was conducted to select the input variable. The soft-sensing model was developed using the multiple linear regression method. The model for the top layer was modified by the correction of dead time. The experimental results showed the temperature inhomogeneity between different layers. The temperature at each target position under two initial temperatures and two flow rates was accurately predicted with a mean absolute error within 0.69 K. Moreover, the temperature under different flow rates can be predicted with one model. Therefore, this soft-sensing model has the potential to be integrated into air-conditioning systems.