bluuebunny/crossref_metadata_2025 huggingface dataset:
Cyclin E/CDK2: DNA Replication, Replication Stress and Genomic Instability
Authors: Fagundes Rafaela , Teixeira Leonardo K.
Published: November 2021

DNA replication must be precisely controlled in order to maintain genome stability. Transition through cell cycle phases is regulated by a family of Cyclin-Dependent Kinases (CDKs) in association with respective cyclin regulatory subunits. In normal cell cycles, E-type cyclins (Cyclin E1 and Cyclin E2, CCNE1 and CCNE2 genes) associate with CDK2 to promote G1/S transition. Cyclin E/CDK2 complex mostly controls cell cycle progression and DNA replication through phosphorylation of specific substrates. Oncogenic activation of Cyclin E/CDK2 complex impairs normal DNA replication, causing replication stress and DNA damage. As a consequence, Cyclin E/CDK2-induced replication stress leads to genomic instability and contributes to human carcinogenesis. In this review, we focus on the main functions of Cyclin E/CDK2 complex in normal DNA replication and the molecular mechanisms by which oncogenic activation of Cyclin E/CDK2 causes replication stress and genomic instability in human cancer.
T-Cell Factors as Transcriptional Inhibitors: Activities and Regulations in Vertebrate Head Development
Authors: Bou-Rouphael Johnny , Durand Béatrice C.
Published: November 2021

Since its first discovery in the late 90s, Wnt canonical signaling has been demonstrated to affect a large variety of neural developmental processes, including, but not limited to, embryonic axis formation, neural proliferation, fate determination, and maintenance of neural stem cells. For decades, studies have focused on the mechanisms controlling the activity of β-catenin, the sole mediator of Wnt transcriptional response. More recently, the spotlight of research is directed towards the last cascade component, the T-cell factor (TCF)/Lymphoid-Enhancer binding Factor (LEF), and more specifically, the TCF/LEF-mediated switch from transcriptional activation to repression, which in both embryonic blastomeres and mouse embryonic stem cells pushes the balance from pluri/multipotency towards differentiation. It has been long known that Groucho/Transducin-Like Enhancer of split (Gro/TLE) is the main co-repressor partner of TCF/LEF. More recently, other TCF/LEF-interacting partners have been identified, including the pro-neural BarH-Like 2 (BARHL2), which belongs to the evolutionary highly conserved family of homeodomain-containing transcription factors. This review describes the activities and regulatory modes of TCF/LEF as transcriptional repressors, with a specific focus on the functions of Barhl2 in vertebrate brain development. Specific attention is given to the transcriptional events leading to formation of the Organizer, as well as the roles and regulations of Wnt/β-catenin pathway in growth of the caudal forebrain. We present TCF/LEF activities in both embryonic and neural stem cells and discuss how alterations of this pathway could lead to tumors.
A Convenient and Label-Free Colorimetric Detection for L-Histidine Based on Inhibition of Oxidation of 3,3′,5,5′-Tetramethylbenzidine-H2O2 System Triggered by Copper Ions
Authors: Zhang Zhikun , Zhao Wenmeng , Hu Cuixia , Cao Yapeng , Liu Yumin , Liu Qingju
Published: November 2021

L-Histidine (L-His) is an essential amino acid, which is used to synthesize proteins and enzymes. The concentration of L-His in the body is controlled to regulate tissue growth and repair of tissues. In this study, a rapid and sensitive method was developed for colorimetric L-his detection using Cu2+ ions to inhibit the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB)–H2O2 system. H2O2 can oxidize TMB to oxTMB in the presence of copper, and the change in color from colorless (TMB) to blue (oxTMB) is similar to that observed in the presence of peroxidase. However, because the imidazole ring and carboxyl group of L-His can coordinate with Cu2+ ions to form stable L-His–Cu2+ complexes, the color of the TMB–H2O2 solution remains unchanged after the addition of L-His. Therefore, because L-His effectively hinders the colorimetric reaction of TMB with H2O2, this assay can be used to quantitatively determine the concentration of L-His in samples. Under optimized conditions, our colorimetric sensor exhibited two linear ranges of 60 nM to 1 μM and 1 μM to 1 mM for L-His detection and a detection limit of 50 nM (S/N = 3); furthermore, the assay can be performed within 20 min. Moreover, the proposed assay was used to determine the concentration of L-His in urine samples, suggesting that this convenient and label-free colorimetric method presents promising applications in bioanalytical chemistry and clinical diagnosis.
Porphyrin Photoabsorption and Fluorescence Variation with Adsorptive Loading on Gold Nanoparticles
Authors: Shinohara Akira , Shao Guang , Nakanishi Takashi , Shinmori Hideyuki
Published: November 2021

Here, we report the photophysical structure–property relationship of porphyrins adsorbed on gold nanoparticles. The number of porphyrin–alkanethiolate adsorbates per particle was adjusted by a post-synthetic thiol/thiolate exchange reaction on 1-dodecanethiolate–protected gold nanoparticles. Even with a low loading level of adsorbates (<10% of all thiolate sites on gold nanoparticles), the shoulder absorption at the Soret band was intensified, indicating the formation of aggregates of porphyrin adsorbates on the nanoparticles. Steady-state fluorescence quantum yields could be adjusted by the bulkiness of substituents at the meso-positions of the porphyrin or the methylene linker chain length, regardless of the porphyrin loading level and the nanoparticle diameter.
Roseburia intestinalis: A Beneficial Gut Organism From the Discoveries in Genus and Species
Authors: Nie Kai , Ma Kejia , Luo Weiwei , Shen Zhaohua , Yang Zhenyu , Xiao Mengwei , Tong Ting , Yang Yuanyuan , Wang Xiaoyan
Published: November 2021

Roseburia intestinalisis an anaerobic, Gram-positive, slightly curved rod-shaped flagellated bacterium that produces butyrate in the colon.R. intestinalishas been shown to prevent intestinal inflammation and maintain energy homeostasis by producing metabolites. Evidence shows that this bacterium contributes to various diseases, such as inflammatory bowel disease, type 2 diabetes mellitus, antiphospholipid syndrome, and atherosclerosis. This review reveals the potential therapeutic role ofR. intestinalisin human diseases. Patients with inflammatory bowel disease exhibit significant changes inR. intestinalisabundance, and they may benefit a lot from modulations targetingR. intestinalis. The data reviewed here demonstrate thatR. intestinalisplays its role in regulating barrier homeostasis, immune cells, and cytokine release through its metabolite butyrate, flagellin and other. Recent advancements in the application of primary culture technology, culture omics, single-cell sequencing, and metabonomics technology have improved research onRoseburiaand revealed the benefits of this bacterium in human health and disease treatment.
Social-Ecological Interactions Influencing Primate Harvest: Insights From Madagascar
Authors: Rivera Christian J. , Mayo Dominic , Hull Vanessa
Published: November 2021

Globally, non-human primates face mounting threats due to unsustainable harvest by humans. There is a need to better understand the diverse drivers of primate harvest and the complex social-ecological interactions influencing harvest in shared human-primate systems. Here, we take an interdisciplinary, systems approach to assess how complex interactions among primate biological and ecological characteristics and human social factors affect primate harvest. We apply our approach through a review and synthesis of the literature on lemur harvest in Madagascar, a country with one of the highest primate species richness in the world coupled with high rates of threatened primate species and populations in decline. We identify social and ecological factors affecting primate harvest, including the characteristics of lemurs that may make them vulnerable to harvest by humans; factors describing human motivations for (or deterrents to) harvest; and political and governance factors related to power and accessibility. We then discuss social-ecological interactions that emerge from: (1) the prevalence of informal institutions (e.g., cultural taboos), (2) adoption of human predatory strategies, (3) synergies with habitat use and habitat loss, and (4) interactions among regional- and local-scale factors (multi-level interactions). Our results illustrate that social-ecological interactions influencing lemur harvest in Madagascar are complex and context-specific, while influenced by a combination of interactions between species-specific characteristics and human social factors. These context-specific interactions may be also influenced by local-level cultural practices, land use change, and effects from regional-level social complexities such as political upheaval and food insecurity. We conclude by discussing the importance of identifying and explicitly accounting for nuances in underlying social-ecological systems and putting forth ideas for future research on primate harvest in shared human-primate systems, including research on social-ecological feedbacks and the application of Routine Activities Theory.
DGAT2-MOGAT2 SNPs and Gene-Environment Interactions on Serum Lipid Profiles and the Risk of Ischemic Stroke
Authors: Zhou Yong-Gang , Yin Rui-Xing , Huang Feng , Wu Jin-Zhen , Chen Wu-Xian , Cao Xiao-Li
Published: November 2021

Background: The genetic susceptibility to ischemic stroke (IS) is still not well-understood. Recent genome-wide association studies (GWASes) found that several single nucleotide polymorphisms (SNPs) in the Diacylglycerol acyltransferase 2 gene (DGAT2) and monoacylglycerol O-acyltransferase 2 (MOGAT2) cluster were associated with serum lipid levels. However, the association between the DGAT2-MOGAT2 SNPs and serum lipid phenotypes has not yet been verified in the Chinese people. Therefore, the present study was to determine the DGAT2-MOGAT2 SNPs and gene-environment interactions on serum lipid profiles and the risk of IS.

Methods: Genotyping of 5 SNPs (DGAT2 rs11236530, DGAT2 rs3060, MOGAT2 rs600626, MOGAT2 rs609379, and MOGAT2 rs10899104) in 544 IS patients and 561 healthy controls was performed by the next-generation sequencing technologies. The association between genotypes and serum lipid data was determined by analysis of covariance, and a corrected P-value was adopted after Bonferroni correction. Unconditional logistic regression analysis was performed to assess the association between genotypes and the risk of IS after adjustment of potential confounders.

Results: The rs11236530A allele was associated with increased risk of IS (CA/AA vs. CC, OR = 1.45, 95%CI = 1.12–1.88, P = 0.0044), whereas the rs600626G-rs609379A-rs10899104G haplotype was associated with decreased risk of IS (adjusted OR = 0.67, 95% CI = 0.48–0.93, P = 0.018). The rs11236530A allele carriers had lower high-density lipoprotein cholesterol (HDL-C) concentrations than the rs11236530A allele non-carriers (P < 0.001). The interactions of rs11236530-smoking, rs3060-smoking and rs10899104-smoking influenced serum apolipoprotein B levels, whereas the interactions of rs11236530- and rs3060-alcohol affected serum HDL-C levels (PI < 0.004–0.001). The interaction of rs600626G-rs609379A-rs10899104G-alcohol (OR = 0.41, 95% CI = 0.22–0.76) and rs600626G-rs609379C-rs10899104T-alcohol (OR = 0.12, 95% CI = 0.04–0.36) decreased the risk of IS (PI < 0.0001).

Conclusions: The rs11236530A allele was associated with decreased serum HDL-C levels in controls and increased risk of IS in patient group. The rs600626G-rs609379A-rs10899104G haplotype, the rs600626G-rs 609379A-rs10899104G-alcohol and rs600626G-rs609379C-rs10899104T-alcohol interactions were associated with decreased risk of IS. The rs11236530 SNP may be a genetic marker for IS in our study populations.
Abnormal Cardiac Repolarization in Thyroid Diseases: Results of an Observational Study
Authors: Aweimer Assem , Schiedat Fabian , Schöne Dominik , Landgrafe-Mende Gabi , Bogossian Harilaos , Mügge Andreas , Patsalis Polykarpos C. , Gotzmann Michael , Akin Ibrahim , El-Battrawy Ibrahim , Dietrich Johannes W.
Published: November 2021

Background: The relationship between thyroid function and cardiac disease is complex. Both hypothyroidism and thyrotoxicosis can predispose to ventricular arrhythmia and other major adverse cardiovascular events (MACE), so that a U-shaped relationship between thyroid signaling and the incidence of MACE has been postulated. Moreover, recently published data suggest an association between thyroid hormone concentration and the risk of sudden cardiac death (SCD) even in euthyroid populations with high-normal FT4 levels. In this study, we investigated markers of repolarization in ECGs, as predictors of cardiovascular events, in patients with a spectrum of subclinical and overt thyroid dysfunction.

Methods: Resting ECGs of 100 subjects, 90 patients (LV-EF > 45%) with thyroid disease (60 overt hyperthyroid, 11 overt hypothyroid and 19 L-T4-treated and biochemically euthyroid patients after thyroidectomy or with autoimmune thyroiditis) and 10 healthy volunteers were analyzed for Tp-e interval. The Tp-e interval was measured manually and was correlated to serum concentrations of thyroid stimulating hormone (TSH), free triiodothyronine (FT3) and thyroxine (FT4).

Results: The Tp-e interval significantly correlated to log-transformed concentrations of TSH (Spearman's rho = 0.30, p < 0.01), FT4 (rho = −0.26, p < 0.05), and FT3 (rho = −0.23, p < 0.05) as well as log-transformed thyroid's secretory capacity (SPINA-GT, rho = −0.33, p < 0.01). Spearman's rho of correlations of JT interval to log-transformed TSH, FT4, FT3, and SPINA-GT were 0.51 (p < 1e−7), −0.45 (p < 1e−5), −0.55 (p < 1e−8), and −0.43 (p < 1e−4), respectively. In minimal multivariable regression models, markers of thyroid homeostasis correlated to heart rate, QT, Tp-e, and JT intervals. Group-wise evaluation in hypothyroid, euthyroid and hyperthyroid subjects revealed similar correlations in all three groups.

Conclusion: We observed significant inverse correlations of Tp-e and JT intervals with FT4 and FT3 over the whole spectrum of thyroid function. Our data suggest a possible mechanism of SCD in hypothyroid state by prolongation of repolarization. We do not observe a U-shaped relationship, so that the mechanism of SCD in patients with high FT4 or hyperthyroidism seems not to be driven by abnormalities in repolarization.
MicroRNAs as Biomarkers and Therapeutic Targets in Doxorubicin-Induced Cardiomyopathy: A Review
Authors: Chen Liuying , Xu Yizhou
Published: November 2021

Doxorubicin is a broad-spectrum chemotherapy drug applied in antitumor therapy. However, its clinical utility is limited by its fatal cardiotoxicity. Doxorubicin (DOX)-induced cardiomyopathy (DIC) begins with the first DOX dose and is characterized by being cumulative dose-dependent, and its early diagnosis using common detection methods is very difficult. Therefore, it is urgent to determine the underlying mechanism of DIC to construct treatment strategies for the early intervention before irreversible damage to the myocardium occurs. Growing evidence suggests that microRNAs (miRNAs) play regulatory roles in the cardiovascular system. miRNAs may be involved in DIC by acting through multiple pathways to induce cardiomyocyte injury. Recent studies have shown that the dysregulation of miRNA expression can aggravate the pathological process of DIC, including the induction of oxidative stress, apoptosis, ion channel dysfunction and microvascular dysfunction. Current findings on the roles of miRNAs in DIC have led to a wide range of studies exploring candidate miRNAs to be utilized as diagnostic biomarkers and potential therapeutic targets for DIC. In this review, we discuss frontier studies on the roles of miRNAs in DIC to better understand their functions, develop relevant applications in DIC, discuss possible reasons for the limitations of their use and speculate on innovative treatment strategies.
Insights Into Endovascular Management of Superior Vena Cava Obstructions
Authors: Ponti Alexandre , Saltiel Sarah , Rotzinger David C. , Qanadli Salah D.
Published: November 2021

Superior vena cava obstruction results from any limitation of blood flow through the superior vena cava. Circulation to the heart may persist through various collateral vessels whose development depends on the level of obstruction. Depending on the level and degree of occlusive disease, the severity of clinical symptoms may vary considerably, up to lethal. Etiologies have changed dramatically in recent years, mainly due to the increasing use of intravascular devices. However, guidelines for treatment are lacking, and various options are available. Endovascular therapies developed considerably in recent years, may offer a rapid improvement in symptoms and proved to be safe. However, knowledge and selection of appropriate techniques are essential to venous angioplasty, involving specific tools to guarantee satisfying outcomes. This review aims to discuss the particular venous anatomy of the upper body, the physiopathology of superior vena cava obstruction, and specificities of endovascular treatment compared with other management options.
MOCOnet: Robust Motion Correction of Cardiovascular Magnetic Resonance T1 Mapping Using Convolutional Neural Networks
Authors: Gonzales Ricardo A. , Zhang Qiang , Papież Bartłomiej W. , Werys Konrad , Lukaschuk Elena , Popescu Iulia A. , Burrage Matthew K. , Shanmuganathan Mayooran , Ferreira Vanessa M. , Piechnik Stefan K.
Published: November 2021

Background: Quantitative cardiovascular magnetic resonance (CMR) T1 mapping has shown promise for advanced tissue characterisation in routine clinical practise. However, T1 mapping is prone to motion artefacts, which affects its robustness and clinical interpretation. Current methods for motion correction on T1 mapping are model-driven with no guarantee on generalisability, limiting its widespread use. In contrast, emerging data-driven deep learning approaches have shown good performance in general image registration tasks. We propose MOCOnet, a convolutional neural network solution, for generalisable motion artefact correction in T1 maps.

Methods: The network architecture employs U-Net for producing distance vector fields and utilises warping layers to apply deformation to the feature maps in a coarse-to-fine manner. Using the UK Biobank imaging dataset scanned at 1.5T, MOCOnet was trained on 1,536 mid-ventricular T1 maps (acquired using the ShMOLLI method) with motion artefacts, generated by a customised deformation procedure, and tested on a different set of 200 samples with a diverse range of motion. MOCOnet was compared to a well-validated baseline multi-modal image registration method. Motion reduction was visually assessed by 3 human experts, with motion scores ranging from 0% (strictly no motion) to 100% (very severe motion).

Results: MOCOnet achieved fast image registration (<1 second per T1 map) and successfully suppressed a wide range of motion artefacts. MOCOnet significantly reduced motion scores from 37.1±21.5 to 13.3±10.5 (p < 0.001), whereas the baseline method reduced it to 15.8±15.6 (p < 0.001). MOCOnet was significantly better than the baseline method in suppressing motion artefacts and more consistently (p = 0.007).

Conclusion: MOCOnet demonstrated significantly better motion correction performance compared to a traditional image registration approach. Salvaging data affected by motion with robustness and in a time-efficient manner may enable better image quality and reliable images for immediate clinical interpretation.
Endothelial Cell CD36 Reduces Atherosclerosis and Controls Systemic Metabolism
Authors: Rekhi Umar R. , Omar Mohamed , Alexiou Maria , Delyea Cole , Immaraj Linnet , Elahi Shokrollah , Febbraio Maria
Published: November 2021

High-fat Western diets contribute to tissue dysregulation of fatty acid and glucose intake, resulting in obesity and insulin resistance and their sequelae, including atherosclerosis. New therapies are desperately needed to interrupt this epidemic. The significant idea driving this research is that the understudied regulation of fatty acid entry into tissues at the endothelial cell (EC) interface can provide novel therapeutic targets that will greatly modify health outcomes and advance health-related knowledge. Dysfunctional endothelium, defined as activated, pro-inflammatory, and pro-thrombotic, is critical in atherosclerosis initiation, in modulating thrombotic events that could result in myocardial infarction and stroke, and is a hallmark of insulin resistance. Dyslipidemia from high-fat diets overwhelmingly contributes to the development of dysfunctional endothelium. CD36 acts as a receptor for pathological ligands generated by high-fat diets and in fatty acid uptake, and therefore, it may additionally contribute to EC dysfunction. We created EC CD36 knockout (CD36°) mice using cre-lox technology and a cre-promoter that does not eliminate CD36 in hematopoietic cells (Tie2e cre). These mice were studied on different diets, and crossed to the low density lipoprotein receptor (LDLR) knockout for atherosclerosis assessment. Our data show that EC CD36° and EC CD36°/LDLR° mice have metabolic changes suggestive of an uncompensated role for EC CD36 in fatty acid uptake. The mice lacking expression of EC CD36 had increased glucose clearance compared with controls when fed with multiple diets. EC CD36° male mice showed increased carbohydrate utilization and decreased energy expenditure by indirect calorimetry. Female EC CD36°/LDLR° mice have reduced atherosclerosis. Taken together, these data support a significant role for EC CD36 in systemic metabolism and reveal sex-specific impact on atherosclerosis and energy substrate use.
Assessment of the Cardiovascular Risk Profile of Infants Exposed to Pre-eclampsia in-utero: A Prospective Case-Control Study in South African Children of African Ancestry
Authors: Nkeh-Chungag Benedicta Ngwenchi , Engwa Godwill Azeh , Businge Charles , Kutllovci-Hasani Kaltrina , Kengne Andre P. , Goswami Nandu
Published: November 2021

Background: It has been reported that maternal gestational environment may be programmed to have a significant impact on foetal and offspring health later in life. Studies have shown that children born to pre-eclamptic mothers are prone to obesity, hypertension, and diabetes in their adult life. However, such findings are yet to be established in an African population. This protocol is for a study aiming to investigate the relationship between pre-eclampsia (PE) and cardiovascular risk in children born to pre-eclamptic mothers in a South African population of African descents.

Methods: A prospective case-control design will be employed to recruit pre-eclamptic and normotensive pregnant women and their offspring after birth. Pregnant women will be assessed for cardiovascular risk factors including PE, obesity, haemodynamics, lipids, glycaemic indices, oxidative stress, and vascular function at 30 weeks of gestation. The cardiovascular risk profile of their offspring will be assessed at birth and 6 weeks later. The difference in cardiovascular risk profile between children born to the pre-eclamptic and normotensive mothers will be compared and the correlation between maternal and offspring cardiovascular risks will be investigated.

Discussion: This will be the first prospective study to assess the in-utero effect of cardiovascular risk in offspring born to pre-eclamptic women of African ancestry. It is expected that findings from this study will provide information on the cardiovascular effect of in-utero exposure to PE in a population of African ancestry. This knowledge will advise policy on the management of women with PE with a view of protecting cardiovascular health in offspring.
Fair and Effective Policing for Neighborhood Safety: Understanding and Overcoming Selection Biases
Authors: Ren Weijeiying , Liu Kunpeng , Zhao Tianxiang , Fu Yanjie
Published: November 2021

An accurate crime prediction and risk estimation can help improve the efficiency and effectiveness of policing activities. However, reports have revealed that biases like racial prejudice could exist in policing enforcement, and trained predictors may inherit them. In this work, we study the possible reasons and countermeasures to this problem, using records from the New York frisk and search program (NYCSF) as the dataset. Concretely, we provide analysis on the possible origin of this phenomenon from the perspective of risk discrepancy, and study it with the scope of selection bias. Motivated by theories in causal inference, we propose a re-weighting approach based on propensity score to balance the data distribution, with respect to the identified treatment: search action. Naively applying existing re-weighting approaches in causal inference is not suitable as the weight is passively estimated from observational data. Inspired by adversarial learning techniques, we formulate the predictor training and re-weighting as a min-max game, so that the re-weighting scale can be automatically learned. Specifically, the proposed approach aims to train a model that: 1) able to balance the data distribution in the searched and un-searched groups; 2) remain discriminative between treatment interventions. Extensive evaluations on real-world dataset are conducted, and results validate the effectiveness of the proposed framework.
A High Resolution Reanalysis for the Mediterranean Sea
Authors: Escudier Romain , Clementi Emanuela , Cipollone Andrea , Pistoia Jenny , Drudi Massimiliano , Grandi Alessandro , Lyubartsev Vladislav , Lecci Rita , Aydogdu Ali , Delrosso Damiano , Omar Mohamed , Masina Simona , Coppini Giovanni , Pinardi Nadia
Published: November 2021

In order to be able to forecast the weather and estimate future climate changes in the ocean, it is crucial to understand the past and the mechanisms responsible for the ocean variability. This is particularly true in a complex area such as the Mediterranean Sea with diverse dynamics like deep convection and overturning circulation. To this end, effective tools are ocean reanalyses or reconstructions of the past ocean state. Here we present a new physical reanalysis of the Mediterranean Sea at high resolution, developed in the Copernicus Marine Environment Monitoring Service (CMEMS) framework. The hydrodynamic model is based on the Nucleus for European Modelling of the Ocean (NEMO) combined with a variational data assimilation scheme (OceanVar). The model has a horizontal resolution of 1/24° and 141 unevenly distributed vertical z* levels. It provides daily and monthly temperature, salinity, current, sea level and mixed layer depth as well as hourly fields for surface velocities and sea level. ECMWF ERA-5 atmospheric fields force the model and daily boundary conditions in the Atlantic are taken from a global reanalysis. The reanalysis covers the 33 years from 1987 to 2019. Initialized from SeaDataNet climatology in January 1985, it reaches a nominal state after a 2-years spin-up. In-situ data from CTD, ARGO floats and XBT are assimilated into the model in combination with satellite altimetry observations. This reanalysis has been validated and assessed through comparison to in-situ and satellite observations as well as literature climatologies. The results show an overall improvement of the comparison with observations and a better representation of the main dynamics of the region compared to a previous, lower resolution (1/16°), reanalysis. Temperature and salinity RMSD are decreased by respectively 14 and 18%. The salinity biases at depth of the previous version are corrected. Climate signals show continuous increase of the temperature and salinity, confirming estimates from observations and other reanalysis. The new reanalysis will allow the study of physical processes at multi-scales, from the large scale to the transient small mesoscale structures and the selection of climate indicators for the basin.
Magmatic-Hydrothermal Processes of Vein-Type Haman-Gunbuk-Daejang Copper Deposits in the Gyeongnam Metallogenic Belt in South Korea
Authors: Lee Tong Ha , Seo Jung Hun , Yoo Bong Chul , Lee Bum Han , Han Seung Hee , Yang Yun Seok , Lee Jun Hee
Published: November 2021

Haman, Gunbuk, and Daejang deposits are neighboring vein-type hydrothermal Cu deposits located in the SE part of the Korean Peninsula. These three deposits are formed by magmatic-hydrothermal activity associated with a series of Cretaceous granodioritic intrusions of the Jindong Granitoids, which have created a series of veins and alterations in a hornfelsed shale formation. The copper deposits have common veining and alteration features: 1) a pervasive chlorite-epidote alteration, cut by 2) Cu-Pb-Zn-bearing quartz veins with a tourmaline-biotite alteration, and 3) the latest barren calcite veins. Chalcopyrite, pyrite, and pyrrhotite are common ore minerals in the three deposits. Whereas magnetite is a dominant mineral in the Haman and Gunbuk deposits, no magnetite is present, but sphalerite and galena are abundant in the Daejang deposit. Ore-bearing quartz veins have three types of fluid inclusions: 1) liquid-rich, 2) vapor-rich, and 3) brine inclusions. Hydrothermal temperatures obtained from the brine inclusion assemblages are about 340–600, 250–500, and 320–460°C in the Haman, Gunbuk, and Daejang deposits, respectively. The maximum temperatures (from 460 to 600°C) recorded in the fluid inclusions of the three deposits are higher than those of the Cu ore precipitating temperature of typical porphyry-like deposits (from 300 to 400°C). Raman spectroscopy of vapor inclusions showed the presence of CO2 and CH4 in the three deposits, which indicates relatively reduced hydrothermal conditions as compared with typical porphyry deposits. The Rb/Sr ratios and Cs concentrations of brine inclusions suggest that the Daejang deposit was formed by a later and more fractionated magma than the Haman and Gunbuk deposits, and the Daejang deposit has lower Fe/Mn ratios in brine inclusions than the Haman and Gunbuk deposits, which indicates contrasting redox conditions in hydrothermal fluids possibly caused by an interaction with a hosting shale formation. In brines, concentrations of base metals do not change significantly with temperature, which suggests that significant ore mineralization precipitation is unlikely below current exposure levels, especially at the Haman deposit. Ore and alteration mineral petrography and fluid inclusions suggest that the Haman deposit was formed near the top of the deep intrusion center, whereas the Gunbuk deposit was formed at a shallower intrusion periphery. The Daejang deposit was formed later at a shallow depth by relatively fractionated magma.
High-Resolution Late Devonian Magnetostratigraphy From the Canning Basin, Western Australia: A Re-Evaluation
Authors: Green Theodore , Slotznick Sarah P. , Jaqueto Plinio , Raub Timothy D. , Tohver Eric , Playton Ted E. , Haines Peter W. , Kirschvink Joseph L. , Hocking Roger M. , Montgomery Paul
Published: November 2021

Late Devonian time was a period of rapid upheaval in the Earth system, including climate change, sea level changes, widespread ocean anoxia, and the Frasnian-Famennian mass extinction; the cause(s) of these changes remain(s) uncertain. The Lennard Shelf of the Canning Basin in Western Australia contains carbonate reef sections spanning much of the Late Devonian Epoch and has been sampled for paleomagnetic analysis with studies by Hansma and colleagues in 2015 and Playton and colleagues in 2016. However, previous paleomagnetic directions were scattered and their use for magnetostratigraphy has been questioned. Here, rock magnetic data and magnetostratigraphy for a late Devonian drill-core from the Lennard Shelf were analyzed. Three magnetostratigraphic interpretations were made using different paleopoles that showed good correlation with each other and the earlier interpretations by Playton and colleagues in 2016. Additionally, the rock magnetic data revealed the samples contain various mixtures of detrital and diagenetic minerals, the former of which should be viable recorders of primary magnetic signatures. Even in samples with these detrital phases, paleomagnetic data were often noisy and produced ambiguous polarity assignments, likely due to the anomalously weak Devonian field. Because of this ambiguity and the absence of a robust paleopole, broader correlations for this critical time-period will be difficult without additional paleomagnetic data from the late Devonian Period. Expanded data for this interval could eventually shed light on the timing, causes, and rates of the Frasnian-Famennian mass extinction and other environmental shifts in the late Devonian Epoch.
Emergency Survey and Stability Analysis of a Rainfall-Induced Soil-Rock Mixture Landslide at Chongqing City, China
Authors: Zhou Chang , Ai Dong , Huang Wei , Xu Huiyuan , Ma Liwen , Chen Lichuan , Wang Luqi
Published: November 2021

The stability analysis of damaged landslides and unstable debris is important for rescue work and emergency operations. This paper investigates a predisposed geological emergence, inducing the factors and deformation processes of the Zhongbao landslide, which happened on July 25, 2020. The stability of the landslide debris was evaluated by an integrated monitoring system consisting of ground-based radar, unmanned aerial vehicles, airborne Lidar, thermal infrared temperature monitoring, GNSS displacement monitoring, deep displacement monitoring, and rainfall monitoring. The strata and weak layer controlled the landslide failure, and topography defined the boundary of the failed rock mass. A continually intensive rainfall caused the deformation and accelerated failure of the landslide. The shallow and steep deposit (Part I) firstly slid at a high velocity, and then pushed the rear part of the landslide (Part II) to deform, forming numerous cracks, which accelerated the rainfall infiltrating into the rock mass. The moisture content increase could decrease the strength of the shale rock within the bedding planes. Finally, with the rock and soil mass sliding along the weak layer, a barrier dam and a barrier lake were formed. The monitoring and numerical simulation results showed that after the landslide failure, there was still local collapse and deformation occurrences which threatened rescue work and barrier lake excavation, and the stability of the accumulation area gradually decreased as the rainfall increased. Therefore, the barrier dam was not excavated until the accumulation rate gradually stabilized on July 28. Moreover, most of the reactivated deposits still accumulated in the transportation and source areas. Thus, in August, the displacement of the landslide debris gradually accelerated in a stepwise manner, and responded strongly to rainfall, especially in the accumulation area, so that it was inferred that the damaged landslide could slide again and cause a more threatening and severe failure. The analysis results of the study area can provide references for the failure mechanism of a rainfall-induced landslide and the stability evaluation of a damaged landslide.
Seismic Response of a Water Transmission Pipeline Across a Fault Zone Adopting a Large-Scale Vibration Table Test
Authors: Deng Longsheng , Zhang Wenzhong , Dai Yan , Fan Wen , Li Yubo , Ren Sen , Li Pei
Published: November 2021

The seismic response is generally amplified significantly near the fault zone due to the influence of discontinuous interfaces and weak-broken geotechnical structures, which imposes a severe geologic hazard risk on the engineering crossing the fault. The Hanjiang to Weihe River Project (phase II) crosses many high seismic intensity regions and intersects with eight large-scale regional active faults. Seismic fortification of the pipelines across the fault zone is significant for the design and construction of the project. A large-scale vibration table test was adopted to investigate the seismic response and fault influences. The responses of accelerations, dynamic stresses, strains, and water pressures were obtained. The results show that the dynamic responses were amplified significantly by the fault zone and the hanging wall. The influence range of fault on acceleration response is approximately four times the fault width. The acceleration amplification ratio in the fault zone generally exceeds 1.35, even reaching 1.8, and the hanging wall amplification ratio is approximately 1.2. The dynamic soil pressure primarily depends on the acceleration distribution and is apparently influenced by pipeline location and model inhomogeneity. The pipeline is bent slightly along the axial direction, accompanied by expansion and shrinkage in the radial direction. The maximum tensile and compressive strains appear at the lower and upper pipeline boundaries near the middle section, respectively. Massive y-direction cracks developed in the soil, accompanied by slight seismic subsidence. The research findings could provide reasonable parameters for the seismic design and construction of the project.
Characteristics and Control Mechanism of Lacustrine Shale Oil Reservoir in the Member 2 of Kongdian Formation in Cangdong Sag, Bohai Bay Basin, China
Authors: Xu Qilu , Zhao Xianzheng , Pu Xiugang , Han Wenzhong , Shi Zhannan , Tian Jinqiang , Zhang Bojie , Xin Bixiao , Guo Pengfei
Published: November 2021

The significance of lacustrine shale oil has gradually become prominent. Lacustrine shale has complex lithologies, and their reservoir properties are quite various. The multi-scale pore structure of shale controls the law of shale oil enrichment. Typical lacustrine shale developed in the Member 2 of Kongdian Formation in Cangdong sag, Bohai Bay Basin. The lithofacies and multi-scale storage space of this lacustrine shale have been systematically studied. 1. The mineral composition is quite different, and the lithofacies can be summarized into siliceous, carbonate and mixed types. The rock structure can be summarized into laminated, layered, and massive types. 2. The pores are diverse and multi-scale. Interparticle pores contribute the main storage space, especially the interparticle pores of quartz and dolomite. 3. The physical properties of the massive shales is relatively inferior to those of layered and laminatedtypes, and it presents the characteristics of " laminated >layered > massive ". The developed laminae can significantly improve the space and seepage capacity of lacustrine shale. 4. Clay minerals provide the main nano-scale storage space, but they are often filled in pores and reduces the shale brittleness, which have destruction effects.
Diabetic Pneumopathy–A New Diabetes-Associated Complication: Mechanisms, Consequences and Treatment Considerations
Authors: Kopf Stefan , Kumar Varun , Kender Zoltan , Han Zhe , Fleming Thomas , Herzig Stephan , Nawroth Peter P.
Published: November 2021

Patients with diabetes are over-represented among the total cases reported with “idiopathic” pulmonary fibrosis (IPF). This raises the question, whether this is an association only or whether diabetes itself can cause pulmonary fibrosis. Recent studies in mouse models of type 1 and type 2 diabetes demonstrated that diabetes causes pulmonary fibrosis. Both types of diabetes trigger a cascade, starting with increased DNA damage, an impaired DNA repair, and leading to persistent DNA damage signaling. This response, in turn, induces senescence, a senescence-associated-secretory phenotype (SASP), marked by the release of pro-inflammatory cytokines and growth factors, finally resulting in fibrosis. Restoring DNA repair drives fibrosis into remission, thus proving causality. These data can be translated clinically to patients with type 2 diabetes, characterized by long-term diabetes and albuminuria. Hence there are several arguments, to substitute the term “idiopathic” pulmonary fibrosis (IPF) in patients with diabetes (and exclusion of other causes of lung diseases) by the term “diabetes-induced pulmonary fibrosis” (DiPF). However, future studies are required to establish this term and to study whether patients with diabetes respond to the established therapies similar to non-diabetic patients.
Risk Factors, Prognostic Factors, and Nomogram for Distant Metastasis in Breast Cancer Patients Without Lymph Node Metastasis
Authors: Min Yu , Liu Xiaoman , Hu Daixing , Chen Hang , Chen Jialin , Xiang Ke , Yin Guobing , Han Yuling , Feng Yang , Luo Haojun
Published: November 2021

Background

Lymph node negative (N0) breast cancer can be found coexisting with distant metastasis (DM), which might consequently make clinicians underestimate the risk of relapse and insufficient treatment for this subpopulation.

Methods

The clinicopathological characteristics of N0 breast cancer patients from the Surveillance, Epidemiology, and End Results (SEER) database between January 2010 and December 2015 were retrospectively reviewed. Multivariate logistic and Cox analyses were used to identify independent risk factors in promoting DM and the 1-, 3-, and 5- year cancer-specific survival (CSS) in this subpopulation.

Result

Seven factors including age (<40 years), tumor size (>10 mm), race (Black), location (central), grade (poor differentiation), histology (invasive lobular carcinoma), and subtype (luminal B and Her-2 enriched) were associated with DM, and the area under curve (AUC) was 0.776 (95% CI: 0.763–0.790). Moreover, T1-3N0M1 patients with age >60 years at diagnosis, Black race, triple-negative breast cancer subtype, no surgery performed, and multiple DMs presented a worse 1-, 3-, and 5-year CSS. The areas under the ROC for 1-, 3-, and 5- year CSS in the training cohort were 0.772, 0.741, and 0.762, respectively, and 0.725, 0.695, and 0.699 in the validation cohort.

Conclusion

The clinicopathological characteristics associated with the risk of DM and the prognosis of female breast cancer patients without lymph node metastasis but with DM are determined. A novel nomogram for predicting 1-, 3-, 5- year CSS in T1-3N0M1 patients is also well established and validated, which could help clinicians better stratify patients who are at a high-risk level for receiving relatively aggressive management.
Sex-Specific Associations of Testosterone and Genetic Factors With Health Span
Authors: Zhao Xiaoyu , Liang Shuang , Wang Nanxi , Hong Tongtong , Sambou Muhammed Lamin , Fan Jingyi , Zhu Meng , Wang Cheng , Hang Dong , Jiang Yue , Dai Juncheng
Published: November 2021

Background

Previous studies have suggested associations between testosterone, genetic factors, and a series of complex diseases, but the associations with the lifespan phenotype, such as health span, remain unclear.

Methods

In this prospective cohort study, we analyzed 145,481 men and 147,733 women aged 38–73 years old from UK Biobank (UKB) to investigate the sex-specific associations of total testosterone (TT), free testosterone (FT), or polygenic risk score (PRS) with health span termination (HST) risk. At baseline, serum testosterone levels were measured. HST was defined by eight events strongly associated with longevity. PRS, an efficient tool combining the effect of common genetic variants to discriminate genetic risk of complex phenotypes, was constructed by 12 single-nucleotide polymorphisms related to health span from UKB (P ≤ 5.0 × 10−8). We used multivariable Cox regression models to estimate hazard ratios (HRs) and 95% confidence intervals (CIs).

Results

With a median follow-up time of 7.70 years, 26,748 (18.39%) men and 18,963 (12.84%) women had HST. TT was negatively associated with HST in men [HR per standard deviation (SD) increment of log-TT: 0.92, 95% CI: 0.88–0.97]. Inversely, both TT (HR per SD increment of log-TT: 1.05, 95% CI: 1.02–1.08) and FT (HR per SD increment of log-FT: 1.08, 95% CI: 1.05–1.11) presented an increased risk of HST in women. PRS was positively associated with HST risk (quintile 5 versus quintile 1, men, HR: 1.19, 95% CI: 1.15–1.24; women, HR: 1.21, 95% CI: 1.16–1.27). Moreover, men with high TT and low genetic risk showed the lowest HST risk (HR: 0.80, 95% CI: 0.73–0.88), whereas HST risk for women with both high TT and genetic risk increased obviously (HR: 1.32, 95% CI: 1.19–1.46). Similar joint effects were observed for FT in both genders.

Conclusions

We observed sex-specific associations that testosterone was negatively associated with HST risk in men and positively associated with HST risk in women. Genetic factors increased the HST risk, suggesting that participants with both high genetic risk and abnormal testosterone levels (high level in women or low level in men) should be the target for early intervention. Although our findings highlight the associations between testosterone and health span, further mechanistic studies and prospective trials are warranted to explore the causation behind.
Short-Term Photovoltaic Power Interval Prediction Based on the Improved Generalized Error Mixture Distribution and Wavelet Packet -LSSVM
Authors: Yang Mao , Peng Tian , Su Xin , Ma Miaomiao
Published: November 2021

The periodicity and non-stationary nature of photovoltaic (PV) output power make the point prediction result contain very little information, increase the difficulty of describing the prediction uncertainty, and it is difficult to ensure the most efficient operation of the power system. Effectively predicting the PV power range will greatly improve the economics and stability of the grid. Therefore, this paper proposes an improved generalized based on the combination of wavelet packet (WP) and least squares support vector machine (LSSVM) to obtain higher accuracy point prediction results. The error mixed distribution function is used to fit the probability distribution of the prediction error, and the probability prediction is performed to obtain the prediction interval. The coverage rate and average width of the prediction interval are used as indicators to evaluate the prediction results of the interval. By comparing with the results of conventional methods based on normal distribution, at 95 and 90% confidence levels, the method proposed in this paper achieves higher coverage while reducing the average bandwidth by 5.238 and 3.756%, which verifies the effectiveness of the proposed probability interval prediction method.
Lignin-Assisted Water Electrolysis for Energy-Saving Hydrogen Production With Ti/PbO2 as the Anode
Authors: Li Jiayi , Zhou Wei , Huang Yuming , Gao Jihui
Published: November 2021

Replacing the oxygen evolution reaction (OER), which is of high energy consumption and slow kinetics, with the more thermodynamically favorable reaction at the anode can reduce the electricity consumption for hydrogen production. Here we developed a lignin-assisted water electrolysis (LAWE) process by using Ti/PbO2 with high OER overpotential as the anode aimed at decreasing the energy consumption for hydrogen production. The influence of key operating parameters such as temperature and lignin concentration on hydrogen production was analyzed. Compared with alkaline water electrolysis (AWE), the anode potential can be decreased from 0.773 to 0.303 (V vs. Hg/HgO) at 10 mA/cm2 in LAWE, and the corresponding cell voltage can be reduced by 546 mV. With increasing the temperature and lignin concentration, current density and H2 production rate were efficiently promoted. Furthermore, the anode deactivation was investigated by analyzing the linear sweep voltammetry (LSV) and cyclic voltammetry (CV) tests. Results showed that the anode deactivation was affected by the temperature.