Recovery of vitamin D levels by cholecalciferol supplementation on obese rats
Authors: Veonika Dea Anenta , Wiboworini Budiyanti , Muthmainah Muthmainah 
Published: May 2024

<p align="center"><strong>ABSTRAK</strong></p><p class="Default"><strong><em>Latar Belakang</em></strong><strong>: </strong>Obesitas disebabkan oleh penumpukan lemak dalam tubuh karena faktor biologis, psikososial, dan perilaku. Prevalensi obesitas mencapai 42,24% di Amerika Serikat, sementara <em>overwe</em><em>ight</em> dan obesitas mencapai 59% di Eropa. Obesitas dapat menyebabkan defisiensi vitamin D melalui berbagai mekanisme.</p><p class="Default"><strong><em>Tujuan:</em></strong> Untuk mengatahui pengaruh pemberian suplementasi <em>cholecalciferol </em>terhadap kadar 25(OH)D pada tikus obesitas.<strong> </strong></p><p class="Default"><strong><em>Metode:</em></strong><strong> </strong>Dalam penelitian ini, kami melakukan penelitian  <em>true experiment with. </em><em>       pre-post test control group design</em>. Penelitian ini menganalisis 3 kelompok tikus galur Sprague Dawley jantan yang diinduksi dengan diet tinggi lemak tinggi fruktosa (HFHF)  menjadi tikus obesitas. Masing-masing kelompok mendapatkan <em>cholecalciferol </em>sebanyak 2,500 IU/200gr/hari pada kelompok P1, 5,000IU/200gr/hari pada kelompok P2, dan 10,000 IU/200gr/hari pada kelompok P3 selama 8 minggu. Tikus kemudian dianalisis kadar serum 25(OH)D sebelum dan sesudah perlakuan.</p><p class="Default"><strong><em>Hasil:</em></strong> Suplementasi <em>cholecalciferol </em>secara signifikan meningkatkan kadar vitamin D pada setiap kelompok intervensi yang diberikan <em>cholecalciferol</em>. Rerata kadar 25(OH)D kelompok P1,P2, dan P3 sebelum perlakuan  berturut-turut adalah 29,43±0,83 ng/mL, 28,61±1,57 ng/mL, dan 28,86±1,46 ng/mL. Rerata kadar 25(OH)D setelah suplementasi <em>cholecalciferol</em> untuk kelompok P1,P2, dan P3 berturut-turut adalah 74,27±0,77 ng/mL, 100,30±1,48 ng/mL, dan 126,73±2,30 ng/mL. Ada perbedaan yang signifikan antara nilai 25(OH)D sebelum dan sesudah perlakuan  pada ketiga kelompok intervensi dengan nilai (p&lt;0,05).<strong> </strong></p><p class="Default"><strong><em>Kesimpula</em></strong><strong><em>n:</em></strong><strong> </strong>Pemberian<strong> </strong><em>cholecalciferol</em> mampu meningkatkan kadar 25(OH)D pada tikus jantan yang diinduksi obesitas.</p><p class="Default"> </p><p><strong>KATA KUNCI: </strong><em>cholecalciferol</em><em>; obesitas; vitamin D</em></p><p align="center"><strong> </strong></p><p align="center"><strong>ABSTRACT</strong><strong> </strong></p><p><strong><em>B</em></strong><strong><em>ackground</em></strong><em>:</em> Obesity is caused by the accumulation of fat in the body due to biological, psychosocial and behavioral factors. The prevalence of obesity reaches 42.24% in the United States, while overweight and obesity reaches 59% in Europe. Obesity can cause vitamin D deficiency through various mechanisms.</p><p><em><strong>Objectives:</strong></em> To determine the effect of cholecalciferol supplementation on 25(OH)D levels in obese mice.</p><p><strong><em>Methods</em></strong><em>:</em> In this research, we conducted a true experiment research with pre-post test control group design. This study analyzed 3 groups of male Sprague Dawley rats that were induced by a high-fat, high-fructose (HFHF) diet to become obese rats. Each group received 2,500 IU/200gr/day of cholecalciferol in group P1, 5,000IU/200gr/day in group P2, and 10,000 IU/200gr/day in group P3 for 8 weeks. The mice were then analyzed for serum 25(OH)D levels before and after treatment.</p><p><strong><em>Results</em></strong><em>:</em> Cholecalciferol supplementation significantly increased vitamin D levels in each intervention group given cholecalciferol. The mean 25(OH)D levels in groups P1, P2, and P3 before treatment were 29.43 ± 0.83 ng/mL, 28.61 ± 1.57 ng/mL, and 28.86 ± 1.46, respectively. ng/mL. The mean 25(OH)D levels after cholecalciferol supplementation for groups P1, P2, and P3 were 74.27±0.77 ng/mL, 100.30±1.48 ng/mL, and 126.73±2 respectively. .30 ng/mL. There was a significant difference between the 25(OH)D values before and after treatment in the three intervention groups with values (p&lt;0.05).</p><p><strong><em>Conclusion</em></strong><em>:</em> Administration of cholecalciferol can increase 25(OH)D levels in male mice that are induced by obesity.</p><p> </p><p class="Default"><strong>KEYWORDS:<em> </em></strong><em>cholecalciferol, vitamin D, obesity<strong></strong></em></p>