bluuebunny/crossref_metadata_2025 huggingface dataset:
A longitudinal study of naloxone opioid overdose awareness and reversal training for first-year medical students: specific elements require reinforcement
Authors: Sandhu Reena K. , Heller Michael V. , Buckanavage Jack , Haslund-Gourley Benjamin , Leckron Joshua , Kupersmith Brady , Goss Nathaniel C. , Samson Kyle , Gadegbeku Annette B.
Published: July 2022

Background

The opioid epidemic is a progressively worsening public health crisis that continues to impact healthcare system strategies such as overdose reversal and destigmatization. Even among healthcare professionals, there remains a lack of confidence in naloxone administration and a prevalence of stigma. While training can play a major impact in reducing these shortcomings, the long-term effectiveness has yet to be characterized in training healthcare professionals. This study examined the long-term retention of opioid overdose awareness and reversal training (OOART) by evaluating performance at two-time intervals, immediately post-training and at a 3-month follow-up.

Methods

Voluntary training was offered to first-year (M1) medical students at the Drexel University College of Medicine in 2021. At this training, 118 students completed training, 95 completed the post-training survey, and 42 completed the 3-month follow-up.

Results

Opioid reversal knowledge questions assessed significantly increased scores post-training and at the 3-month follow-up. In three of the attitude questions, scores were improved at both follow-up timepoints. In addition, three attitude questions indicating a participant’s confidence to respond to an opioid overdose situation increased directly after the training, but regressed at the 3-month follow-up. The remaining questions did not show any statistical difference across the survey intervals.

Conclusions

This study establishes that while OOART provides participants with the knowledge of how to respond to an opioid overdose, the retention of this knowledge at a 3-month interval is reduced. The results were mixed for longitudinal assessment of participant’s attitudes toward people with opioid use disorder. Some positive increases in attitudes were retained at the 3-month interval, while others trended back toward pre-training levels. These results support the effectiveness of the training but also provide evidence that OOART must be reinforced often.
Follicle-stimulating hormone signaling in Sertoli cells: a licence to the early stages of spermatogenesis
Authors: Wang Jia-Ming , Li Zhen-Fang , Yang Wan-Xi , Tan Fu-Qing
Published: July 2022

Follicle-stimulating hormone signaling is essential for the initiation and early stages of spermatogenesis. Follicle-stimulating hormone receptor is exclusively expressed in Sertoli cells. As the only type of somatic cell in the seminiferous tubule, Sertoli cells regulate spermatogenesis not only by controlling their own number and function but also through paracrine actions to nourish germ cells surrounded by Sertoli cells. After follicle-stimulating hormone binds to its receptor and activates the follicle-stimulating hormone signaling pathway, follicle-stimulating hormone signaling will establish a normal Sertoli cell number and promote their differentiation. Spermatogonia pool maintenance, spermatogonia differentiation and their entry into meiosis are also positively regulated by follicle-stimulating hormone signaling. In addition, follicle-stimulating hormone signaling regulates germ cell survival and limits their apoptosis. Our review summarizes the aforementioned functions of follicle-stimulating hormone signaling in Sertoli cells. We also describe the clinical potential of follicle-stimulating hormone treatment in male patients with infertility. Furthermore, our review may be helpful for developing better therapies for treating patients with dysfunctional follicle-stimulating hormone signaling in Sertoli cells.
Repopulated microglia induce expression of Cxcl13 with differential changes in Tau phosphorylation but do not impact amyloid pathology
Authors: Karaahmet Berke , Le Linh , Mendes Monique S. , Majewska Ania K. , O’Banion M. Kerry
Published: July 2022

Background

Adult microglia rely on self-renewal through division to repopulate and sustain their numbers. However, with aging, microglia display morphological and transcriptional changes that reflect a heightened state of neuroinflammation. This state threatens aging neurons and other cells and can influence the progression of Alzheimer’s disease (AD). In this study, we sought to determine whether renewing microglia through a forced partial depletion/repopulation method could attenuate AD pathology in the 3xTg and APP/PS1 mouse models.

Methods

We pharmacologically depleted the microglia of two cohorts of 21- to 22-month-old 3xTg mice and one cohort of 14-month-old APP/PS1 mice using PLX5622 formulated in chow for 2 weeks. Following depletion, we returned the mice to standard chow diet for 1 month to allow microglial repopulation. We assessed the effect of depletion and repopulation on AD pathology, microglial gene expression, and surface levels of homeostatic markers on microglia using immunohistochemistry, single-cell RNAseq and flow cytometry.

Results

Although we did not identify a significant impact of microglial repopulation on amyloid pathology in either of the AD models, we observed differential changes in phosphorylated-Tau epitopes after repopulation in the 3xTg mice. We provide evidence that repopulated microglia in the hippocampal formation exhibited changes in the levels of homeostatic microglial markers. Lastly, we identified novel subpopulations of microglia by performing single-cell RNAseq analysis on CD45int/+cells from hippocampi of control and repopulated 3xTg mice. In particular, one subpopulation induced after repopulation is characterized by heightened expression ofCxcl13.

Conclusion

Overall, we found that depleting and repopulating microglia causes overexpression of microglialCxcl13with disparate effects on Tau and amyloid pathologies.
A retrospective analysis of serous effusions based on the newly proposed international system for reporting serous fluid cytopathology: a report of 3633 cases in an oncological center
Authors: Zhu Yan-li , Ren Wen-hao , Wang Qian , Jin Hai-zhu , Guo Yi-yi , Lin Dong-mei
Published: July 2022

Background

The International System for Reporting Serous Fluid Cytopathology (TIS) was recently proposed. We retrospectively applied TIS recommendations for reporting the cytological diagnosis of serous effusions and reported our experience.

Methods

All the serous effusions from January 2018 to September 2021 were retrieved from the database. Recategorization was performed using the TIS classification, the risk of malignancy (ROM) was calculated for each TIS category. In addition, on the basis of the original TIS classification, we further subdivided the TIS category IV (suspicious for malignancy, SFM) into 2 groups (IVa and IVb) according to cytological characteristics (quality and quantity) to explore the necessity of SFM subclassification. The performance evaluation was carried out using different samples (pleural, peritoneal and pericardial effusions) and preparation methods (conventional smears, liquid-based preparations and cell blocks).

Results

A total of 3633 cases were studied: 17 (0.5%) were diagnosed as ‘nondiagnostic’ (I, ND), 1100 (30.3%) as ‘negative for malignancy’ (II, NFM), 101 (2.8%) as ‘atypia of undetermined significance’ (III, AUS), 677 (18.6%) as ‘suspicious for malignancy’ (IV, SFM), and 1738 (47.8%) as ‘malignant’ (V, MAL). The ROMs for the categories were 38.5%, 28.6%, 52.1%, 99.4% and 100%, respectively. The ROM for SFM was significantly higher than that for AUS (P < 0.001), while the difference between the ROMs for IVa and IVb was insignificant. The sensitivity, negative predictive value (NPV) and diagnostic accuracy of liquid-based preparations were all superior to those of conventional smears and cell blocks in detecting abnormalities. Using the three preparation methods simultaneously had the highest sensitivity, NPV and diagnostic accuracy.

Conclusion

Serous effusion cytology has a high specificity and positive predictive value (PPV), and TIS is a user-friendly reporting system. Liquid-based preparations could improve the sensitivity of diagnosis, and it is best to use three different preparation methods simultaneously for serous effusion cytologic examination.
Increased spine PIP3 is sequestered from dendritic shafts
Authors: Ueda Yoshibumi , Sugimoto Naotoshi , Ozawa Takeaki
Published: July 2022

Phosphatidylinositol 3,4,5-trisphosphate (PIP3) is a lipid second messenger that is crucial for the synaptic plasticity underlying learning and memory in pyramidal neurons in the brain. Our previous study uncovered PIP3 enrichment in the dendritic spines of hippocampal pyramidal neurons in the static state using a fluorescence lifetime-based PIP3 probe. However, the extent to which PIP3 enrichment is preserved in different states has not been fully investigated. Here, we revealed that PIP3 accumulation in dendritic spines is strictly controlled even in an active state in which PIP3 is increased by glutamate stimulation and high potassium-induced membrane depolarization. Time-course PIP3 analysis clarified the gradual PIP3 accumulation in dendritic spines over days during neuronal development. Collectively, these results deepen our understanding of PIP3 dynamics in dendritic spines, and the dysregulation of the PIP3 gradient between dendritic spines and shafts could cause neuronal diseases and mental disorders, such as autism spectrum disorder.
Genetic diversity of drug-resistant Mycobacterium tuberculosis clinical isolates from Khuzestan province, Iran
Authors: Bakhtiyariniya Pejman , Khosravi Azar Dokht , Hashemzadeh Mohammad , Savari Mohammad
Published: July 2022

The emergence of drug-resistant strains of the Mycobacterium tuberculosis (MTB) has challenged tuberculosis control programs. So far, few studies using the 24-locus mycobacterial interspersed repetitive unit variable number tandem repeats (MIRU-VNTR) have investigated the genetic diversity of MTB in Iran. This study aimed to determine the genetic diversity of MTB isolates resistant to first-line anti-tuberculosis drugs using 24-locus MIRU-VNTR in southwestern Iran. Out of 6620 MTB clinical isolates, 29 resistant isolates to one or more isoniazid, rifampin, and ethambutol were detected using drug susceptibility testing by the proportional method. The manual 24-locus MIRU-VNTR was used to determine the MTB resistant isolates’ phylogenetic relationship. MIRU-VNTRplus web application tools were applied to analyze the associated data. Using 24-locus MIRU-VNTR, 13.8% of isolates (n = 4) were distributed in two clusters, and the remaining 86.2% (n = 25) showed a unique pattern. Four clonal complexes were observed in the minimum spanning tree based on the double-locus variant. Most isolates belonged to Delhi/CAS (34.5%, 10/29) and NEW-1 (24.1%, 7/29) sub-lineages, followed by EAI and LAM with a frequency of 6.9% (2/29) and 3.5% (1/29), respectively. Eight isolates (27.6%) did not match any genotype in the database. The 24-locus MIRU-VNTR showed a high discriminatory power; however, the 15-locus and 12-locus set analyses were more discriminative. Our study revealed a high degree of genetic diversity among drug-resistant MTB isolates, which could be interpreted as the low rate of person-to-person transmission in this region. The 15-locus MIRU-VNTR would be recommended for preliminary genotyping of drug-resistant MTB.
How to boost the boosters? A survey-experiment on the effectiveness of different policies aimed at enhancing acceptance of a “Seasonal” vaccination against COVID-19
Authors: Goren Talia , Beeri Itai , Vashdi Dana Rachel
Published: July 2022

Background

Evidence suggests a gradual decrease in the effectiveness of the anti-COVID-19 vaccines, stressing the potential need for periodical booster shots. However, it is hard to tell whether previously applied policies for enhancing vaccine acceptance will be as effective for repeated periodical booster shots during a pandemic. Hence, this study aims to explore the effectiveness of different health policies on periodical vaccination acceptance amidst an ongoing pandemic.

Methods

A cross sectional online experiment was performed in a representative sample of 929 Israeli citizens. Participants were randomly allocated to 4 groups simulating different hypothetical periodical-vaccination-promoting policy scenarios: (1) Mandate (N = 229); (2) a negative monetary incentive (N = 244); (3) a positive monetary incentive (N = 228) and (4) information provision (N = 228). Compliance intentions and vaccine-acceptance-related variables were measured. Analysis included multivariate hierarchic logistic and linear regressions.

Results

Compliance intentions levels were medium (M = 3.13 on a 1–5 scale). Only 20.2% of the sample demonstrated strong acceptance of periodical vaccination, which is lower than the acceptance rate of the seasonal flu shot in the country in the year preceding the pandemic. Type of policy was related to the extent to which a respondent strongly agreed to be periodically vaccinated or not. Specifically, strong acceptance was more likely when positive or negative incentives were presented in comparison to the mandate or information provision conditions. However, when examining the extent of compliance among respondents who were less decisive, the type of policy did not predict the extent to which these respondents intended to comply. In addition, compliance intentions were related with the perceived benefits and barriers of the vaccine, the perceived efficacy of getting vaccinated and social norms. Hesitator’s intentions were additionally associated with anti-COVID-19 vaccination history, perceived severity of the disease and trust in government.

Conclusions

Pandemic-containing vaccines may be perceived as less effective and beneficial than pandemic-preventing vaccines. Individuals with different levels of motivation for periodical vaccination during a pandemic may be affected by different factors. While strongly opinionated individuals are affected by the type of vaccination-promoting policy, hesitators are affected by a larger number of factors, which provide policy makers with greater opportunities to enhance their vaccination intentions.
Survival benefits from neoadjuvant treatment in gastric cancer: a systematic review and meta-analysis
Authors: Hu Jianwen , Yang Yanpeng , Ma Yongchen , Ning Yingze , Chen Guowei , Liu Yucun
Published: July 2022

Background

Surgery is the main treatment option for patients with local gastric cancer. However, surgery alone is usually not sufficient for stomach cancer patients, and combined therapies are recommended for these patients. In recent studies, some preoperative treatments have shown benefits. However, the treatment selection is still uncertain because previous studies failed to obtain a statistically significant difference between preoperative chemotherapy and preoperative chemoradiotherapy. Therefore, we plan to perform a systematic review and meta-analysis to compare the benefits among these preoperative treatments.

Methods/design

This review includes randomized controlled trials with or without blinding as well as published studies, high-quality unpublished studies, full articles and meeting abstracts with an English context if sufficient results were provided for analysis. Data sources include the Cochrane Central Register of Controlled Trials, Embase, MEDLINE, major relevant international conferences and manual screening of references. Patients with a diagnosis of resectable primary gastric or EGJ adenocarcinoma (stage II or higher) who underwent surgery alone or preoperative treatment followed by surgery and who were pathologically confirmed as proposed by the AJCC 2017 guidelines without age, sex, race, subtypes of adenocarcinoma and molecular pathology limitations will be included. The following three interventions will be included: surgery alone, neoadjuvant chemistry followed by surgery and neoadjuvant chemoradiotherapy followed by surgery. All-cause mortality, overall survival (OS, the time interval from diagnosis to death) and/or progression-free survival (PFS, the time interval from diagnosis to disease progression or death from any cause) will be defined as major results of concern. The clinical and pathological response rate (according to RECIST and tumour regression score), R0 resection rate, quality of life and grade 3 or above adverse events (according to the National Cancer Institute Common Terminology Criteria for Adverse Events, NCI-CTCAE) will be defined as the secondary outcomes.

Discussion

The aim of this systematic review is to compare the benefits of different preoperative treatments for patients with locoregional stomach cancer. This systematic review will improve the understanding of the relative efficacy of these treatment options by providing the latest evidence on the efficacy of various treatment options in the management of gastric cancer patients and may guide clinical practice.

Systematic review registration

PROSPERO CRD4202123718
Investigation of technical quality of antenatal and perinatal services in a nationally representative sample of health facilities in Nepal
Authors: Khatri Resham B. , Durham Jo , Assefa Yibeltal
Published: July 2022

Background

Access to routine antenatal and perinatal services is improved in the last two decades in Nepal. However, gaps remain in coverage and quality of care delivered from the health facilities. This study investigated the delivery of technical quality antenatal and perinatal services from health facilities and their associated determinants in Nepal.

Methods

Data for this study were derived from the Nepal Health Facility Survey 2015. The World Health Organization's Service Availability and Readiness Assessment framework was adopted to assess the technical quality of antenatal and perinatal services of health facilities. Outcome variables included technical quality scores of i) 269 facilities providing antenatal services and ii) 109 facilities providing childbirth and postnatal care services (perinatal care). Technical quality scores of health facilities were estimated adapting recommended antenatal and perinatal interventions. Independent variables included locations and types of health facilities and their management functions (e.g., supervision). We conducted a linear regression analysis to identify the determinants of better technical quality of health services in health facilities. Beta coefficients were exponentiated into odds ratios (ORs) and reported with 95% confidence intervals (CIs). The significance level was set at p-value < 0.05.

Results

The mean score of the technical quality of health facilities for each outcome variable (antenatal and perinatal services) was 0.55 (out of 1.00). Compared to province one, facilities of Madhesh province had 4% lower odds (adjusted OR = 0.96; 95%CI: 0.92, 0.99) of providing better quality antenatal services, while health facilities of Gandaki province had higher odds of providing better quality antenatal services (aOR = 1.05; 95% CI: 1.01, 1.10). Private facilities had higher odds (aOR = 1.13; 95% CI: 1.03, 1.23) of providing better quality perinatal services compared to public facilities.

Conclusions

Private facilities provide better quality antenatal and perinatal health services than public facilities, while health facilities of Madhesh province provide poor quality perinatal services. Health system needs to implement tailored strategies, including recruiting health workers, supervision and onsite coaching and access to necessary equipment and medicine in the facilities of Madhesh province. Health system inputs (trained human resources, equipment and supplies) are needed in the public facilities. Extending the safe delivery incentive programme to the privately managed facilities could also improve access to better quality health services in Nepal.
CircCEP85 upregulates IGF1 expression to promote breast cancer progression via sponging miR-1193
Authors: Gao Fei , Han Jianjun , Jia Li , He Jun , Wang Yun , Chen Mi
Published: July 2022

Background

Increasing evidence has suggested that circular RNAs (circRNAs) play critical roles in breast cancer (BC) progression. However, the expression level and potential functional role of circRNA centrosomal protein 85 (circCEP85) in BC remains largely unknown. Here, we aimed to explore the role of circCEP85 in BC.

Methods

The levels of circCEP85, insuline-like growth factor I (IGF1) mRNA and microRNA-1193 (miR-1193) were examined by quantitative real-time polymerase chain reaction. The protein level was measured by Western blot. Cell proliferation, migration, apoptosis, angiogenesis and stemness were assessed by cell counting kit-8, 5-ethynyl-2’-deoxyuridine assay, transwell assay, flow cytometry, tube formation and sphere formation assays. Xenograft mouse models were conducted to evaluate the effect of circCEP85 in BC in vivo. Moreover, dual-luciferase reporter, RNA pull-down, and RNA immunoprecipitation (RIP) assays were preformed to confirm the interaction between miR-1193 and circCEP85 or IGF1.

Results

CircCEP85 was upregulated in BC tissues and cells. Silencing of circCEP85 inhibited proliferation, invasion, angiogenesis and stemness, but promoted apoptosis in BC cells in vitro. In addition, circCEP85 silencing inhibited tumor growth in vivo. Mechanistically, circCEP85 elevated IGF1 expression via sponging miR-1193 to promote breast cancer progression.

Conclusion

The circCEP85-miR-1193-IGF1 axis regulated BC progression via the competitive endogenous RNA (ceRNA) mechanism. CircCEP85 might be a prognostic biomarker and therapeutic target for BC.
Biopsies of osseous jaw lesions using 3D-printed surgical guides: a clinical study
Authors: Postl Lukas , Mücke Thomas , Hunger Stefan , Wuersching Sabina Noreen , Holberg Svenia , Bissinger Oliver , Burgkart Rainer , Malek Michael , Krennmair Stefan
Published: July 2022

Background

Bone biopsies are often necessary to make a diagnosis in the case of irregular bone structures of the jaw. A 3D-printed surgical guide may be a helpful tool for enhancing the accuracy of the biopsy and for ensuring that the tissue of interest is precisely removed for examination. This study was conducted to compare the accuracy of biopsies performed with 3D-printed surgical guides to that of free-handed biopsies.

Methods

Computed tomography scans were performed on patients with bony lesions of the lower jaw. Surgical guides were planned via computer-aided design and manufactured by a 3D-printer. Biopsies were performed with the surgical guides. Bone models of the lower jaw with geometries identical to the patients’ lower jaws were produced using a 3D-printer. The jaw models were fitted into a phantom head model and free-handed biopsies were taken as controls. The accuracy of the biopsies was evaluated by comparing the parameters for the axis, angle and depth of the biopsies to the planned parameters.

Results

Eight patients were included. The mean deviation between the biopsy axes was significantly lower in guided procedures than in free-handed biopsies (1.4 mm ± 0.9 mm; 3.6 mm ± 1.0 mm; p = 0.0005). The mean biopsy angle deviation was also significantly lower in guided biopsies than in free-handed biopsies (6.8° ± 4.0; 15.4° ± 3.6; p = 0.0005). The biopsy depth showed no significant difference between the guided and the free-handed biopsies.

Conclusions

Computer-guided biopsies allow significantly higher accuracy than free-handed procedures.
Global metabolic alterations in colorectal cancer cells during irinotecan-induced DNA replication stress
Authors: Marx Christian , Sonnemann Jürgen , Maddocks Oliver D. K. , Marx-Blümel Lisa , Beyer Mandy , Hoelzer Doerte , Thierbach René , Maletzki Claudia , Linnebacher Michael , Heinzel Thorsten , Krämer Oliver H.
Published: July 2022

Background

Metabolic adaptations can allow cancer cells to survive DNA-damaging chemotherapy. This unmet clinical challenge is a potential vulnerability of cancer. Accordingly, there is an intense search for mechanisms that modulate cell metabolism during anti-tumor therapy. We set out to define how colorectal cancer CRC cells alter their metabolism upon DNA replication stress and whether this provides opportunities to eliminate such cells more efficiently.

Methods

We incubated p53-positive and p53-negative permanent CRC cells and short-term cultured primary CRC cells with the topoisomerase-1 inhibitor irinotecan and other drugs that cause DNA replication stress and consequently DNA damage. We analyzed pro-apoptotic mitochondrial membrane depolarization and cell death with flow cytometry. We evaluated cellular metabolism with immunoblotting of electron transport chain (ETC) complex subunits, analysis of mitochondrial mRNA expression by qPCR, MTT assay, measurements of oxygen consumption and reactive oxygen species (ROS), and metabolic flux analysis with the Seahorse platform. Global metabolic alterations were assessed using targeted mass spectrometric analysis of extra- and intracellular metabolites.

Results

Chemotherapeutics that cause DNA replication stress induce metabolic changes in p53-positive and p53-negative CRC cells. Irinotecan enhances glycolysis, oxygen consumption, mitochondrial ETC activation, and ROS production in CRC cells. This is connected to increased levels of electron transport chain complexes involving mitochondrial translation. Mass spectrometric analysis reveals global metabolic adaptations of CRC cells to irinotecan, including the glycolysis, tricarboxylic acid cycle, and pentose phosphate pathways. P53-proficient CRC cells, however, have a more active metabolism upon DNA replication stress than their p53-deficient counterparts. This metabolic switch is a vulnerability of p53-positive cells to irinotecan-induced apoptosis under glucose-restricted conditions.

Conclusion

Drugs that cause DNA replication stress increase the metabolism of CRC cells. Glucose restriction might improve the effectiveness of classical chemotherapy against p53-positive CRC cells.

Graphical Abstract

The topoisomerase-1 inhibitor irinotecan and other chemotherapeutics that cause DNA damage induce metabolic adaptations in colorectal cancer (CRC) cells irrespective of their p53 status. Irinotecan enhances the glycolysis and oxygen consumption in CRC cells to deliver energy and biomolecules necessary for DNA repair and their survival. Compared to p53-deficient cells, p53-proficient CRC cells have a more active metabolism and use their intracellular metabolites more extensively. This metabolic switch creates a vulnerability to chemotherapy under glucose-restricted conditions for p53-positive cells.
The influence of learning mode and learning sharing behavior on the synchronicity of attention of sharers and learners
Authors: Xiaojun Zhao , Xinrui Kang , Xupeng Li
Published: July 2022

Attention is the concentration of mental activities to a certain object, and students' inattentiveness in class directly affects their learning efficiency. As an emerging technology of educational application, augmented reality (AR) technology combines virtual reality and three-dimensional reconstruction to bring multisensory stimulation to students, enhancing immersion and attention in learning. A quantitative study was conducted on third-grade pupils. Study 1 examined whether learning mode and learning sharing behavior affect the synchronization of sharers’ and learners’ attention. Study 2 examined the impact of learning mode and sharing role on sharer and shared. The results showed that compared with learning alone, when sharing, the attention score of AR group is higher than that of text group. Whether it is the sharer or the shared, the attention score of AR group is higher than that of text group. AR has more advantages than text in terms of learning attention. In future research, it is optional to diversify AR learning materials and further use near-infrared spectroscopy technology to study interactive learning in AR mode.
Determination of the polymer composition of mid-twentieth century purses by Raman spectroscopy
Authors: Boyden Mary N. , Kleist Elyse M. , Asztalos Courtney K. , Korter Timothy M.
Published: July 2022

A database was constructed of 134 reference plastic samples and their Raman spectra to aid in the rapid and accurate identification of the polymer composition of mid-twentieth century plastic purses and their component parts from the Plastics Artifacts Collection at Syracuse University Libraries. Work began by making and testing the database, which led to the determination that matching artifact spectra to reference sample spectra worked well for artifact spectra having signal-to-noise ratios down to 30:1. This finding allowed for using reduced laser power as necessary to protect delicate and pigmented artifacts. These studies enabled the definitive chemical identification of seven selected artifacts, showing that the majority are composed of single plastics including polystyrene, poly(methyl methacrylate), or cellulose acetate. However, it was discovered that one of the purses was composed of both poly(methyl methacrylate) and cellulose acetate. The compositions of three artifacts of unknown composition were discovered, corrections to the existing records were made for three other artifacts, and the composition of one artifact was confirmed. This decisive study will contribute to the archival processing and preservation of these artifacts.
Utilization of a meteorological satellite as a space telescope: the lunar mid-infrared spectrum as seen by Himawari-8
Authors: Nishiyama Gaku , Namiki Noriyuki , Sugita Seiji , Uno Shinsuke
Published: July 2022

The Japanese meteorological satellite Himawari-8 has captured the Earth’s atmosphere and stars, planets, and the Moon in its field of view, enabling us to capture their spectroscopy with 16 bands from visible to mid-infrared wavelengths. The nine infrared bands in the Advanced Himawari Imager (AHI) onboard Himawari-8 are unique in spaceborne observations and are potentially useful for lunar science. In addition, infrared bands of AHI cover wavelengths similar to those of other interplanetary instruments and thus are useful for calibrations. However, infrared AHI data have not yet been investigated in planetary science. To confirm the utility of AHI for planetary science, we develop a procedure to retrieve the lunar infrared spectrum and compare it with thermal conduction simulations. Our analysis shows that lunar brightness temperature curves can be obtained in the morning, evening, and nighttime for all nine bands. Particularly at 8.5 μm, they show a good agreement with previous observations by the Diviner radiometer onboard NASA’s Lunar Reconnaissance Orbiter. As pointed out previously, the brightness temperatures differ between the bands, indicating temperature mixing within a pixel. Our simulation suggests that surface roughness as steep as those measured at the Apollo landing sites significantly contributes to the observed brightness temperature differences in the morning and evening; however, nighttime brightness temperatures are greatly affected by rocks with higher thermal inertia than the regolith. The rock abundances are estimated to be 0.18–0.48% and 6.1–10.3% at the equator and within Tycho crater, respectively. Our estimations from AHI data are consistent with those of Diviner. These results support the idea that AHI potentially serves as a space telescope for future lunar and planetary sciences, for example, for constraining water content on the lunar surface.

Graphical Abstract
Wide-field swept-source OCT angiography of the periarterial capillary-free zone before and after anti-VEGF therapy for branch retinal vein occlusion
Authors: Tang Wenyi , Liu Wei , Guo Jingli , Zhang Lili , Xu Gezhi , Wang Keyan , Chang Qing
Published: July 2022

Background

The aim of the study was to investigate the changes in the periarterial capillary-free zone (paCFZ) after anti-vascular endothelial growth factor (VEGF) therapy in patients with branch retinal vein occlusion (BRVO) by wide-field swept-source optical coherence tomography angiography (SS-OCTA) and assess their associations with clinical outcomes.

Methods

In this retrospective observational study of 54 treatment-naïve BRVO patients with macular edema, we reviewed the findings of 12 × 12 mm2 SS-OCTA at baseline, 3, 6, and 12 months after intravitreal ranibizumab injections. The paCFZ and major retinal artery areas were measured on SS-OCTA images. The paCFZ area to artery area (P/A) ratio was calculated.

Results

The paCFZ areas and P/A ratios of first- and second-order arteries were significantly greater in BRVO eyes than in contralateral eyes (all P < 0.01), but there were no differences in the first- and second-order artery areas (P = 0.20 and 0.25, respectively). The paCFZ areas and P/A ratios decreased significantly at 3, 6, and 12 months after anti-VEGF therapy (all P < 0.01). The baseline P/A ratio was significantly correlated with the baseline best-corrected visual acuity (BCVA), central retinal thickness, and their improvements at 3, 6, and 12 months (all P < 0.05). Baseline BCVA and P/A ratios of first- and second-order arteries were independently associated with the final BCVA in multivariate linear regression.

Conclusions

Wide-field SS-OCTA shows that anti-VEGF therapy can lead to a significant improvement in the paCFZ parameters in BRVO. Smaller baseline P/A ratios on SS-OCTA tend to predict better visual outcomes at 12 months after anti-VEGF therapy.
Support structure tomography using per-pixel signed shadow casting in human manikin 3D printing
Authors: Jung Jin Young , Chee Seonkoo , Sul In Hwan
Published: July 2022

This study proposes an advanced algorithm for predicting the optimal orientation in human manikin 3D printing. We can print the manikin mesh data on any scale depending on the user’s needs. Once the 3D printing scale was determined, the manikin data were dissected based on the 3D printer’s maximal printing volume using our previous work. Then, we applied the newly proposed algorithm, designated as “per-pixel signed-shadow casting,” to each dissected manikin part to calculate the volumes of the object and the support structure. Our method classified the original mesh triangles into three groups—alpha, beta, and top-covering—to eliminate the need for special hardware such as graphic cards. The result is shown as a two-dimensional bitmap file, designated as “tomograph”. This tomograph represents the local support structure distribution information on a visual and quantitative basis. Repeating this tomography method for the three rotational axes resulted in a four-dimensional (4D) box-shaped graph. The optimal orientation of any arbitrary object is easily determined from the lowest-valued pixel in the 4D box graph. We applied this proposed method to several basic primitive shapes with different degrees of symmetry and complex shapes, such as the famous “Stanford Bunny”. Finally, the algorithm was applied to human manikins using several printing scales. The theoretical values were compared with those obtained from analytical or g-code-based experimental volumes.
Active monitoring of adverse reactions following COVID-19 and other vaccinations: a feasibility study as part of the CoVaKo project
Authors: Zeschick Nikoletta , Warkentin Lisette , Kühlein Thomas , Steininger Philipp , Überla Klaus , Hueber Susann , Sebastião Maria
Published: July 2022

Background

The Corona-Vakzin-Konsortium project (CoVaKo) analyses the efficacy and safety of COVID-19 vaccines in a real-world setting, as well as breakthrough infections in Bavaria, Germany. A subproject of CoVaKo aims to identify adverse reactions of the COVID-19 vaccine and compare these to adverse reactions of other vaccines in an online survey. In a preceding feasibility study, the study materials were tested for comprehensibility, visual design, and motivation to participate, as well as for their ability to be implemented and carried out in primary care practices and vaccination centres.

Methods

We used a mixed-methods research design. First, three focus groups consisting of general population participants were organised to evaluate the study materials and survey. Second, a test roll-out was conducted in vaccination centres and primary care practices that involved implementing and quantitatively evaluating the online survey. Third, interviews were conducted with participating general practitioners and heads of vaccination centres four weeks after the test roll-out.

Results

Parts of the information and registration form proved incomprehensible, specifically regarding the recruitment material and/or online survey. For example, headings were misleading given that, relative to other vaccinations, the COVID-19 vaccination was overemphasised in the title. Participants requested additional information regarding the procedure and completion time. Within 31 days, 2199 participants, who received either a COVID-19 vaccination (99%) or at least one of the control vaccinations (1%), registered for the study. Participants (strongly) agreed that the registration process was easy to understand, that the completion time was reasonable, and that the technical setup was straightforward. Physicians and heads of the vaccination centres perceived the study as easy to integrate into their workflow. The majority expressed willingness to participate in the main study.

Conclusions

Our study indicated that identifying and documenting adverse reactions following vaccinations using an online survey is feasible. Testing materials and surveys provided valuable insight, enabling subsequent improvements. Participation from health professionals proved essential in ensuring the practicality of procedures. Lastly, adapting the study’s organisation to external fluctuating structures and requirements confirmed necessary for a successful implementation, especially due to dynamic changes in the nation’s COVID-19 vaccination strategies.

Trial registration

The trial was retrospectively registered at the “Deutsches Register Klinischer Studien” (DRKS-ID: DRKS00025881) on Oct 14, 2021.
Systematic review of the characteristics of school-based feasibility cluster randomised trials of interventions for improving the health of pupils in the UK
Authors: Parker Kitty , Eddy Saskia , Nunns Michael , Xiao ZhiMin , Ford Tamsin , Eldridge Sandra , Ukoumunne Obioha C.
Published: July 2022

Background

The last 20 years have seen a marked increase in the use of cluster randomised trials (CRTs) in schools to evaluate interventions for improving pupil health outcomes. Schools have limited resources and participating in full-scale trials can be challenging and costly, given their main purpose is education. Feasibility studies can be used to identify challenges with implementing interventions and delivering trials. This systematic review summarises methodological characteristics and objectives of school-based cluster randomised feasibility studies in the United Kingdom (UK).

Methods

We systematically searched MEDLINE from inception to 31 December 2020. Eligible papers were school-based feasibility CRTs that included health outcomes measured on pupils.

Results

Of 3285 articles identified, 24 were included. School-based feasibility CRTs have been increasingly used in the UK since the first publication in 2008. Five (21%) studies provided justification for the use of the CRT design. Three (13%) studies provided details of a formal sample size calculation, with only one of these allowing for clustering. The median (IQR; range) recruited sample size was 7.5 (4.5 to 9; 2 to 37) schools and 274 (179 to 557; 29 to 1567) pupils. The most common feasibility objectives were to estimate the potential effectiveness of the intervention (n = 17; 71%), assess acceptability of the intervention (n = 16; 67%), and estimate the recruitment/retention rates (n = 15; 63%). Only one study was used to assess whether cluster randomisation was appropriate, and none of the studies that randomised clusters before recruiting pupils assessed the possibility of recruitment bias. Besides potential effectiveness, cost-effectiveness, and the intra-cluster correlation coefficient, no studies quantified the precision of the feasibility parameter estimates.

Conclusions

Feasibility CRTs are increasingly used in schools prior to definitive trials of interventions for improving health in pupils. The average sample size of studies included in this review would be large enough to estimate pupil-level feasibility parameters (e.g., percentage followed up) with reasonable precision. The review highlights the need for clearer sample size justification and better reporting of the precision with which feasibility parameters are estimated. Better use could be made of feasibility CRTs to assess challenges that are specific to the cluster design.

Trial registration

PROSPERO: CRD42020218993.
The inconsistency of documentation: a study of online C standard library documents
Authors: Li Ruishi , Yang Yunfei , Liu Jinghua , Hu Peiwei , Meng Guozhu
Published: July 2022

The C standard libraries are basic function libraries standardized by the C language. Programmers usually refer to their API documentation provided by third-party websites. Unfortunately, these documents are not necessarily complete or accurate, especially for constraint sentences of API usage, which are called Security Specifications (SSs). SS issues can prevent programmers from following obligatory constraints, which results in API misuse vulnerabilities. Previous work studying SS issues could only find certain types of inaccurate SSs through checking the compliance between API usage and existing SSs. Therefore, we propose a novel approach SSeeker for quickly discovering missing and inaccurate SSs through the inconsistency of semantically similar SSs. More specifically, SSeeker first completes broken sentences and discovers SSs from them by judging their constraint sentiment. Then SSeeker puts semantically similar SSs from different sources into a group, which can be used to discover missing or inaccurate SSs. With the help of SSeeker, we investigated 4 popular online third-party C standard library documents, studied their conformity with the C99 standard, analyzed their APIs and SSs, and discovered 92 prototype issues, 15 web page issues, and 96 SS issues.
Frequency changes during the propagation of a light beam
Authors: Emile Olivier , Emile Janine
Published: July 2022

The energy and therefore the frequency of electromagnetic fields are quantities which are generally considered to be conserved in free space propagation. However, we observe here a frequency varying optical wave in free space propagation. The light comes from an Arago spot. Its frequency changes due to the rotational Doppler effect. More precisely, the light diffracted by a two-dimensional asymmetric object carries orbital angular momentum that varies with distance. As the object rotates, the frequency of the diffracted light experiences different rotational Doppler shifts along propagation. It varies in a discrete quantized way as it propagates. It can be adjusted, without violation of the conservation of energy. Since this phenomenon is deeply rooted in the diffraction process, it sheds some new light on this still open issue.
Palmar Striated Xanthomas in Clinical Practice
Authors: Roy Nathalie , Gaudet Daniel , Brisson Diane
Published: July 2022

Context

Palmar striated xanthomas (PSX) are macular subcutaneous lesions conferring a yellow-to-orange coloration of palmar and finger creases that characterize dysbetalipoproteinemia, a disease associated with sustained plasma accumulation of triglyceride-rich lipoprotein remnants. Although remnants accumulation may occur in any condition interfering with triglyceride-rich lipoprotein hydrolysis or clearance, the presence of PSX has not been systematically assessed across the spectrum of lipid disorders potentially associated with sustained or recurrent remnants accumulation.

Objective

The aim of this study was to assess the occurrence of (PSX) in a wide spectrum of lipid disorders ranging from very severe hypercholesterolemia (homozygous familial hypercholesterolemia) to very severe hypertriglyceridemia (chylomicronemia).

Methods

This study involved 3382 dyslipidemic White adult patients (1856 men and 1526 women) seen at the Chicoutimi Hospital Lipid Clinic (Quebec, Canada), covering a wide range of lipid disorders, from severe hypertriglyceridemia to severe hypercholesterolemia. Categorical variables were compared using the Pearson χ 2 statistic, whereas univariate analysis of variance or nonparametric Kruskal-Wallis was used for continuous variables.

Results

A total of 5.1% (173/3382) of the studied patients presented PSX, a majority of them (67.1%) being women. PSX were observed in 18.8% of patients with dysbetalipoproteinemia and also among 14.1% of hypertriglyceridemic patients with partial lipoprotein lipase deficiency, 3.7% of patients with chylomicronemia, and in all those with homozygous familial hypercholesterolemia. Overall, 10.7% of patients with PSX did not meet dysbetalipoproteinemia diagnosis criteria.

Conclusion

According to our study, the PSX prevalence estimate among patients without dysbetalipoproteinemia would be around 10% and they could be observed in a wide spectrum of lipid disorders associated with recurrent or sustained remnant lipoprotein accumulation.
Temporomandibular joint disorders prevalence and awareness of appropriate clinical practices, among Al-Madinah community: A prospective cross sectional study
Authors: Alolayan Albraa , Alsayed Shayma S. , Salamah Ruwaa M. , Ali Khadija M. , Alsousi Mashael , Elsayed Shadia
Published: July 2022

Background:  Painful temporomandibular joint disorders (TMDs) are of musculoskeletal origin and are considered the most common cause of non-odontogenic pain in the orofacial region.  The purpose of this study was to investigate the prevalence and awareness of temporomandibular joint (TMJ) disorders in Almadinah Almunawwarah community.

Methods: A prospective observational cross-sectional study with convenience sampling was conducted. A translated Arabic version of Fonseca's questionnaire was employed. The questionnaire asked about the participant's personal information, if they thought they had TMDs, and who to visit for therapy if necessary. These were followed by 10 items from Fonseca's questionnaire, each with a three-point scale.

Results: The questionnaire was completed by 598 people. Females made up 57.1% of the participants. TMDs were present in 61% of the population, with varying degrees of severity. Males (44.3%) were less affected than females (55.7%). The difference, however, was not statistically significant (P = 0.354). Out of the 61% TMDs Positive patients, 74.1% had mild TMDs symptoms, while 20.8% and 5.1%, respectively, had moderate and severe TMDs symptoms (P = 0.05). The severity of the symptoms was unaffected by demographic data (P > 0.05). Only 40% seek care, with 64.6% selecting for a dentist and 24.6% preferring for an orthopaedic specialist (P= 0.008).

Conclusions: Participants from Al-Madinah had a greater prevalence of mild TMDs. The majority of the participants had no idea who to go to for treatment. The findings of this study highlight the importance of educational activities to enhance public awareness