bluuebunny/crossref_metadata_2025 huggingface dataset:
Entrapment of Binaural Auditory Beats in Subjects with Symptoms of Insomnia
Authors: Lee Eunyoung , Bang Youngrong , Yoon In-Young , Choi Ha-Yun
Published: March 2022

Binaural beat (BB) stimulation, which has two different frequencies for each ear, is reportedly effective in reducing anxiety and controlling mood. This study aimed to evaluate the brain wave entrainment effect of binaural beats and to propose an effective and safe supplementary therapy for relieving the symptoms of insomnia. Subjects between 20 and 59 years of age with subclinical symptoms of insomnia were recruited from the community. Quantitative electroencephalography was measured twice, before and two weeks after the BB intervention. Participants used the apparatus with or without 6 Hz BB for 30 min before going to bed for two weeks. When music with BB was played, the relative theta power increased (occipital, p = 0.009). After two weeks of intervention with music, the theta power increased when listening to music with BB (parietal, p = 0.009). After listening to music with BB for two weeks, the decrease in the beta power was more noticeable than after using music-only devices when participants listened to music in the laboratory (occipital, p = 0.035). When BB were played, the entrapment of the theta wave appeared. Therefore, exposure to music with BB is likely to reduce the hyper-arousal state and contribute to sleep induction.
Editorial: Neurosurgery and Neuroanatomy
Authors: Yağmurlu Kaan
Published: March 2022

Microsurgical anatomy is not only the backbone for neurosurgical operations, but also for technological innovations, novel surgical techniques, a better understanding of the etiopathogenesis of pathologies, and translational medicine from neuroscience to daily clinical practice [...]
Rumination and Worry Selectively Modulate Total Calorie Consumption within an Online, Nudge Tactic Paradigm
Authors: Eschle Timothy M. , Wale Sarah P. , McCarrick Dane
Published: March 2022

Rumination and worry, collectively referred to as perseverative cognition, have been implicated in the increased engagement of several health risk behaviours. The current study aimed to investigate the potential influencing role of these repetitive negative thought cognitions in an online snack paradigm. Participants were randomly assigned to either an even condition (a 3:3 ratio of ≤101 kcal and >201 kcal snacks) or an uneven condition (a 4:2 ratio in favour of ≤101 kcal snacks). Upon the presentation of six images of sweet treats, participants were asked to choose the snack they most wanted to consume “right now”, before completing the Ultra-Brief Penn State Worry Questionnaire (UB-PSWQ) and the brief (5-item) Ruminative Response Scale (RRS). The results showed that the reduced availability of higher calorie snacks significantly improved both snack choice and total calorie consumption. However, despite rumination and worry having no influence on the snack type chosen, higher levels of rumination still led to significantly higher overall calorie consumption. Although, contrary to predictions, higher levels of worry conversely led to significantly lower overall calorie consumption. This study adds to the growing work in the role of perseverative cognition and food consumption, which may aid in informing public health strategies. Further exploration is needed to assess whether rumination directly induces unhealthy eating behaviours or simply exacerbates them.
Measurable Residual Disease in High-Risk Acute Myeloid Leukemia
Authors: Cluzeau Thomas , Lemoli Roberto M. , McCloskey James , Cooper Todd
Published: March 2022

Mounting evidence suggests measurable residual disease (MRD) assessments are prognostic in acute myeloid leukemia (AML). High-risk AML encompasses a subset of AML with poor response to therapy and prognosis, with features such as therapy-related AML, an antecedent hematologic disorder, extramedullary disease (in adults), and selected mutations and cytogenetic abnormalities. Historically, few patients with high-risk AML achieved deep and durable remission with conventional chemotherapy; however, newer agents might be more effective in achieving MRD-negative remission. CPX-351 (dual-drug liposomal encapsulation of daunorubicin/cytarabine at a synergistic ratio) demonstrated MRD-negativity rates of 36–64% across retrospective studies in adults with newly diagnosed high-risk AML and 84% in pediatric patients with first-relapse AML. Venetoclax (BCL2 inhibitor) demonstrated MRD-negativity rates of 33–53% in combination with hypomethylating agents for high-risk subgroups in studies of older adults with newly diagnosed AML who were ineligible for intensive therapy and 65% in combination with chemotherapy in pediatric patients with relapsed/refractory AML. However, there is no consensus on optimal MRD methodology in AML, and the use of different techniques, sample sources, sensitivity thresholds, and the timing of assessments limit comparisons across studies. Robust MRD analyses are needed in future clinical studies, and MRD monitoring should become a routine aspect of AML management.
Metformin and ICG-001 Act Synergistically to Abrogate Cancer Stem Cells-Mediated Chemoresistance in Colorectal Cancer by Promoting Apoptosis and Autophagy
Authors: Roy Souvick , Zhao Yinghui , Yuan Yate-Ching , Goel Ajay
Published: March 2022

Colorectal cancer (CRC) remains the third most frequently diagnosed cancer in the United States. The current treatment regimens for CRC include surgery followed by 5FU-based chemotherapy. Cancer stem-like cells (CSCs) have been implicated in 5FU-mediated chemoresistance, which leads to poor prognosis. In this study, we used metformin along with ICG-001, a Wnt signaling inhibitor, to abrogate CSC-mediated chemoresistance in CRC. We observed that 5FU-resistant (5FUR) CRC cells exhibited increased expression of CSC markers and enhanced spheroid formation. Genome-wide transcriptomic profiling analysis revealed that Wnt signaling, colorectal cancer metastasis signaling, etc., were enriched in 5FUR CRC cells. Accordingly, selective targeting of Wnt signaling using ICG-001 along with metformin abrogated CSC-mediated chemoresistance by decreasing the expression of CSC markers and promoting autophagy and apoptosis in a synergistic manner. We also observed that metformin and ICG-001 exhibited anti-tumor activity in CRC patient-derived tumor organoids. In conclusion, our study highlights that metformin and ICG-001 act synergistically and can be used as part of a therapeutic strategy to overcome 5FU-mediated therapeutic resistance in CRC.
Translational Regulation by hnRNP H/F Is Essential for the Proliferation and Survival of Glioblastoma
Authors: Le Bras Morgane , Gorelick Noah , Pautet Sylvain , Tyler Betty , Manenti Stéphane , Skuli Nicolas , Millevoi Stefania , Cammas Anne
Published: March 2022

Deregulation of mRNA translation is a widespread characteristic of glioblastoma (GBM), aggressive malignant brain tumors that are resistant to conventional therapies. RNA-binding proteins (RBPs) play a critical role in translational regulation, yet the mechanisms and impact of these regulations on cancer development, progression and response to therapy remain to be fully understood. Here, we showed that hnRNP H/F RBPs are potent regulators of translation through several mechanisms that converge to modulate the expression and/or the activity of translation initiation factors. Among these, hnRNP H/F regulate the phosphorylation of eIF4E and its translational targets by controlling RNA splicing of the A-Raf kinase mRNA, which in turn modulates the MEK-ERK/MAPK signaling pathway. The underlying mechanism involves RNA G-quadruplex (RG4s), RNA structures whose modulation phenocopies hnRNP H/F translation regulation in GBM cells. Our results highlighted that hnRNP H/F are essential for key functional pathways regulating proliferation and survival of GBM, highlighting its targeting as a promising strategy for improving therapeutic outcomes.
PIK3CA Gene Mutations in HNSCC: Systematic Review and Correlations with HPV Status and Patient Survival
Authors: Cochicho Daniela , Esteves Susana , Rito Miguel , Silva Fernanda , Martins Luís , Montalvão Pedro , Cunha Mário , Magalhães Miguel , Gil da Costa Rui M. , Felix Ana
Published: March 2022

PIK3CA mutations are believed to contribute to the pathogenesis of human papillomavirus (HPV)-associated head and neck squamous cell carcinomas (HNSCC). This study aims to establish the frequency of PIK3CA mutations in a Portuguese HNSCC cohort and to determine their association with the HPV status and patient survival. A meta-analysis of scientific literature also revealed widely different mutation rates in cohorts from different world regions and a trend towards improved prognosis among patients with PIK3CA mutations. DNA samples were available from 95 patients diagnosed with HNSCC at the Portuguese Institute of Oncology in Lisbon between 2010 and 2019. HPV status was established based on viral DNA detected using real-time PCR. The evaluation of PIK3CA gene mutations was performed by real-time PCR for four mutations (H1047L; E542K, E545K, and E545D). Thirty-seven cases were found to harbour PIK3CA mutations (39%), with the E545D mutation (73%) more frequently detected. There were no significant associations between the mutational status and HPV status (74% WT and 68% MUT were HPV (+); p = 0.489) or overall survival (OS) (3-year OS: WT 54% and MUT 65%; p = 0.090). HPV status was the only factor significantly associated with both OS and disease-free survival (DFS), with HPV (+) patients having consistently better outcomes (3-year OS: HPV (+) 65% and HPV (−) 36%; p = 0.007; DFS HPV (+) 83% and HPV (−) 43%; p = 0.001). There was a statistically significant interaction effect between HPV status and PIK3CA mutation regarding DFS (Interaction test: p = 0.026). In HPV (+) patients, PIK3CA wild-type is associated with a significant 4.64 times increase in the hazard of recurrence or death (HR = 4.64; 95% CI 1.02–20.99; p = 0.047). Overall, PIK3CA gene mutations are present in a large number of patients and may help define patient subsets who can benefit from therapies targeting the PI3K pathway. The systematic assessment of PIK3CA gene mutations in HNSCC patients will require further methodological standardisation.
Stacking Machine Learning Algorithms for Biomarker-Based Preoperative Diagnosis of a Pelvic Mass
Authors: Shaw Reid , Lokshin Anna E. , Miller Michael C. , Messerlian-Lambert Geralyn , Moore Richard G.
Published: March 2022

Objective: To identify the most predictive parameters of ovarian malignancy and develop a machine learning (ML) based algorithm to preoperatively distinguish between a benign and malignant pelvic mass. Methods: Retrospective study of 70 predictive parameters collected from 140 women with a pelvic mass. The women were split into a 3:1 “training” to “testing” dataset. Feature selection was performed using Gini impurity through an embedded random forest model and principal component analysis. Nine unique ML classifiers were assessed across a variety of model-specific hyperparameters using 25 bootstrap resamples of the training data. Model predictions were then combined into an ensemble stack by LASSO regression. The final ensemble stack and individual classifiers were then applied to the testing dataset to assess model performance. Results: Feature selection identified HE4, CA125, and transferrin as three predictive parameters of malignancy. Assessment of the ensemble stack on the testing dataset outperformed all individual ML classifiers in predicting malignancy. The ensemble stack demonstrated an accuracy of 97.1%, a receiver operating characteristic (ROC) area under the curve (AUC) of 0.951, and a sensitivity of 93.3% with a specificity of 100%. Conclusions: Combining the measurement of three distinct biomarkers with the stacking of multiple ML classifiers into an ensemble can provide valuable preoperative diagnostic predictions for patients with a pelvic mass.
Therapeutic Approaches in Adult Primary Spinal Cord Astrocytoma: A Systematic Review
Authors: Anghileri Elena , Broggi Morgan , Mazzapicchi Elio , Farinotti Mariangela , Botturi Andrea , Tramacere Irene , Marchetti Marcello
Published: March 2022

The issue: Gliomas are primary tumors arising from supporting cells of the central nervous system (CNS), usually in the brain. The 2021 World Health Organization (WHO) classifies gliomas as adult-type diffuse gliomas or circumscribed astrocytic gliomas depending on their histology and molecular features. Spinal astrocytic gliomas are very rare, and nowadays no standard of therapy is available. Treatment options are limited: surgery is often not radical, and adjuvant therapies include mostly radiotherapy (RT) or systemic chemotherapy (CHT). There is lack of knowledge about the efficacy and safety of therapies and their multidisciplinary approaches. The aim of the review: A systematic review of the literature from January 2000 to June 2021 was performed, including both clinical trials and observational studies on histological adult primary spinal cord astrocytomas (SCA), with a minimum follow-up of 6 months and reporting the overall survival, progression-free survival or clinical neurological outcome after any therapeutic approach (surgery, RT or CHT). What are the main findings? A total of 1197 citations were identified by the Medline search and additional records; based on our inclusion criteria, 18 studies were included with a total of 285 adult patients. We documented the lack of any clinical trial. What are the conclusions? The available literature data are limited to series/retrospective studies, including heterogeneous patients, i.e., astrocytoma as well as ependymoma or pediatric/adult age, with scanty data on the outcomes of interest. No clinical trials have been run. Due to the rarity of this disease, multicentric clinical trials with molecular investigations are mandatory to better manage such a rare disease.
Multi-Disciplinary Care Planning of Ovarian Cancer in Older Patients: General Statement—A Position Paper from SOFOG-GINECO-FRANCOGYN-SFPO
Authors: Bengrine Leila , Bakrin Naoual , Rousseau Frédérique , Lavoué Vincent , Falandry Claire
Published: March 2022

In this position paper the Société Francophone d’OncoGériatrie (SOFOG; French-speaking oncogeriatric society), the Société Française de Pharmacie Oncologique (SFPO, French society for oncology pharmacy), the Groupe d’Investigateurs Nationaux pour l’Étude des Cancers de l’Ovaire et du sein (GINECO, National Investigators’ Group for Studies in Ovarian and Breast Cancer) and the Groupe Français de chirurgie Oncologique et Gynécologique (FRANCOGYN) propose a multi-disciplinary care planning of ovarian cancer in older patients. The treatment pathway is based on four successive decisional nodes (diagnosis, resectability assessment, operability assessment, adjuvant, and maintenance treatment decision) implying multidisciplinarity and adaptation of the treatment plan according to the patient’s geriatric covariates and her motivation towards treatment. Specific attention must be paid to geriatric intervention, supportive care and pharmaceutical conciliation. Studies are needed to prospectively evaluate the impact of geriatric vulnerability parameters at each step of the treatment agenda and the impact of geriatric interventions on patient outcomes.
Editorial Catalysts: Catalysis for the Removal of Gas-Phase Pollutants
Authors: Palomares Antonio Eduardo
Published: March 2022

Air pollution is one of the greatest concerns affecting the world today [...]
High Mobility Group Box 1: Biological Functions and Relevance in Oxidative Stress Related Chronic Diseases
Authors: Taverna Simona , Tonacci Alessandro , Ferraro Maria , Cammarata Giuseppe , Cuttitta Giuseppina , Bucchieri Salvatore , Pace Elisabetta , Gangemi Sebastiano
Published: March 2022

In the early 1970s, a group of non-histone nuclear proteins with high electrophoretic mobility was discovered and named high-mobility group (HMG) proteins. High-mobility group box 1 (HMGB1) is the most studied HMG protein that detects and coordinates cellular stress response. The biological function of HMGB1 depends on its subcellular localization and expression. It plays a critical role in the nucleus and cytoplasm as DNA chaperone, chromosome gatekeeper, autophagy maintainer, and protector from apoptotic cell death. HMGB1 also functions as an extracellular alarmin acting as a damage-associated molecular pattern molecule (DAMP). Recent findings describe HMGB1 as a sophisticated signal of danger, with a pleiotropic function, which is useful as a clinical biomarker for several disorders. HMGB1 has emerged as a mediator in acute and chronic inflammation. Furthermore, HMGB1 targeting can induce beneficial effects on oxidative stress related diseases. This review focus on HMGB1 redox status, localization, mechanisms of release, binding with receptors, and its activities in different oxidative stress-related chronic diseases. Since a growing number of reports show the key role of HMGB1 in socially relevant pathological conditions, to our knowledge, for the first time, here we analyze the scientific literature, evaluating the number of publications focusing on HMGB1 in humans and animal models, per year, from 2006 to 2021 and the number of records published, yearly, per disease and category (studies on humans and animal models).
The Effects of Prenatal Exposure to Pregabalin on the Development of Ventral Midbrain Dopaminergic Neurons
Authors: Alsanie Walaa F. , Alhomrani Majid , Gaber Ahmed , Habeeballah Hamza , Alkhatabi Heba A. , Felimban Raed I. , Abdelrahman Sherin , Hauser Charlotte A. E. , Chaudhary Adeel G. , Alamri Abdulhakeem S. , Raafat Bassem M. , Alamri Abdulwahab , Anwar Sirajudheen , Alswat Khaled A. , Althobaiti Yusuf S. , Asiri Yousif A.
Published: March 2022

Pregabalin is widely used as a treatment for multiple neurological disorders; however, it has been reported to have the potential for misuse. Due to a lack of safety studies in pregnancy, pregabalin is considered the last treatment option for various neurological diseases, such as neuropathic pain. Therefore, pregabalin abuse in pregnant women, even at therapeutic doses, may impair fetal development. We used primary mouse embryonic neurons to investigate whether exposure to pregabalin can impair the morphogenesis and differentiation of ventral midbrain neurons. This study focused on ventral midbrain dopaminergic neurons, as they are responsible for cognition, movement, and behavior. The results showed that pregabalin exposure during early brain development induced upregulation of the dopaminergic progenitor genes Lmx1a and Nurr1 and the mature dopaminergic gene Pitx3. Interestingly, pregabalin had different effects on the morphogenesis of non-dopaminergic ventral midbrain neurons. Importantly, our findings illustrated that a therapeutic dose of pregabalin (10 μM) did not affect the viability of neurons. However, it caused a decrease in ATP release in ventral midbrain neurons. We demonstrated that exposure to pregabalin during early brain development could interfere with the neurogenesis and morphogenesis of ventral midbrain dopaminergic neurons. These findings are crucial for clinical consideration of the use of pregabalin during pregnancy.
A Novel LHX6 Reporter Cell Line for Tracking Human iPSC-Derived Cortical Interneurons
Authors: Cruz-Santos Maria , Cardo Lucia Fernandez , Li Meng
Published: March 2022

GABAergic interneurons control the neural circuitry and network activity in the brain. The dysfunction of cortical interneurons, especially those derived from the medial ganglionic eminence, contributes to neurological disease states. Pluripotent stem cell-derived interneurons provide a powerful tool for understanding the etiology of neuropsychiatric disorders, as well as having the potential to be used as medicine in cell therapy for neurological conditions such as epilepsy. Although large numbers of interneuron progenitors can be readily induced in vitro, the generation of defined interneuron subtypes remains inefficient. Using CRISPR/Cas9-assisted homologous recombination in hPSCs, we inserted the coding sequence of mEmerald and mCherry fluorescence protein, respectively, downstream that of the LHX6, a gene required for, and a marker of medial ganglionic eminence (MGE)-derived cortical interneurons. Upon differentiation of the LHX6-mEmerald and LHX6-mCherry hPSCs towards the MGE fate, both reporters exhibited restricted expression in LHX6+ MGE derivatives of hPSCs. Moreover, the reporter expression responded to changes of interneuron inductive cues. Thus, the LHX6-reporter lines represent a valuable tool to identify molecules controlling human interneuron development and design better interneuron differentiation protocols as well as for studying risk genes associated with interneuronopathies.
The Future of the COVID-19 Pandemic: How Good (or Bad) Can the SARS-CoV2 Spike Protein Get?
Authors: Nugent Matthew A.
Published: March 2022

Severe acute respiratory syndrome virus 2 (SARS-CoV2) has infected an estimated 400 million people world-wide, causing approximately 6 million deaths from severe coronavirus disease 2019 (COVID-19). The SARS-CoV2 Spike protein plays a critical role in viral attachment and entry into host cells. The recent emergence of highly transmissible variants of SARS-CoV2 has been linked to mutations in Spike. This review provides an overview of the structure and function of Spike and describes the factors that impact Spike’s ability to mediate viral infection as well as the potential limits to how good (or bad) Spike protein can become. Proposed here is a framework that considers the processes of Spike-mediated SARS-CoV2 attachment, dissociation, and cell entry where the role of Spike, from the standpoint of the virus, is to maximize cell entry with each viral-cell collision. Key parameters are identified that will be needed to develop models to identify mechanisms that new Spike variants might exploit to enhance viral transmission. In particular, the importance of considering secondary co-receptors for Spike, such as heparan sulfate proteoglycans is discussed. Accurate models of Spike-cell interactions could contribute to the development of new therapies in advance of the emergence of new highly transmissible SARS-CoV2 variants.
TSPO Deficiency Exacerbates GSDMD-Mediated Macrophage Pyroptosis in Inflammatory Bowel Disease
Authors: Zhang Xue , Han Jingyi , Xu Yi , Cai Menghua , Gao Fei , Han Jiajia , Wang Dongdong , Fu Yi , Chen Hui , He Wei , Zhang Jianmin
Published: March 2022

Background: the 18-kDa translocator protein (TSPO) is a mitochondrial outer membrane protein, and its expression tends to increase in response to inflammatory stimulation, rapidly. However, the role of TSPO in inflammation and pyroptosis is not yet clear. Here, we identified TSPO as a novel key regulator of pyroptosis. (2) Methods: TSPO knockout and DSS induced mouse inflammatory bowel disease (IBD) models were employed to assess the roles of TSPO in the pathogenesis of IBD. Primary peritoneal macrophages from TSPO knockout mice were applied to evaluate the mechanism of TSPO in cell pyroptosis. Conclusions: in response to inflammatory injury, TSPO expression is rapidly upregulated and provides a protective function against GSDMD-mediated pyroptosis, which helps us better understand the biological role of TSPO and a novel regulatory mechanism of the pyroptosis process.
Inhibitory Neurotransmission Is Sex-Dependently Affected by Tat Expression in Transgenic Mice and Suppressed by the Fatty Acid Amide Hydrolase Enzyme Inhibitor PF3845 via Cannabinoid Type-1 Receptor Mechanisms
Authors: Xu Changqing , Yadav-Samudrala Barkha J. , Xu Callie , Nath Bhupendra , Mistry Twisha , Jiang Wei , Niphakis Micah J. , Cravatt Benjamin F. , Mukhopadhyay Somnath , Lichtman Aron H. , Ignatowska-Jankowska Bogna M. , Fitting Sylvia
Published: March 2022

(1) Background. The endocannabinoid (eCB) system, which regulates physiological and cognitive processes, presents a promising therapeutic target for treating HIV-associated neurocognitive disorders (HAND). Here we examine whether upregulating eCB tone has potential protective effects against HIV-1 Tat (a key HIV transactivator of transcription) protein-induced alterations in synaptic activity. (2) Methods. Whole-cell patch-clamp recordings were performed to assess inhibitory GABAergic neurotransmission in prefrontal cortex slices of Tat transgenic male and female mice, in the presence and absence of the fatty acid amide hydrolase (FAAH) enzyme inhibitor PF3845. Western blot and mass spectrometry analyses assessed alterations of cannabinoid receptor and enzyme protein expression as well as endogenous ligands, respectively, to determine the impact of Tat exposure on the eCB system. (3) Results. GABAergic activity was significantly altered upon Tat exposure based on sex, whereas the effectiveness of PF3845 to suppress GABAergic activity in Tat transgenic mice was not altered by Tat or sex and involved CB1R-related mechanisms that depended on calcium signaling. Additionally, our data indicated sex-dependent changes for AEA and related non-eCB lipids based on Tat induction. (4) Conclusion. Results highlight sex- and/or Tat-dependent alterations of GABAergic activity and eCB signaling in the prefrontal cortex of Tat transgenic mice and further increase our understanding about the role of FAAH inhibition in neuroHIV.
Mast Cells and Acupuncture Analgesia
Authors: Li Yingchen , Yu Yi , Liu Yuhang , Yao Wei
Published: March 2022

Mast cells are widely distributed in various parts of the human body and play a vital role in the progression of many diseases. Recently, the close relationship between mast cells and acupoints was elucidated, and the role of mast cells in acupuncture analgesia has attracted the attention of researchers worldwide. Using mast cells, acupuncture analgesia and acupoint as key words to search CNKI, PubMed, Web of Science and other databases, combining the representative articles in these databases with the published research papers of our group, we summarized: The enrichment of mast cells and the dense arrangement of collagen fibers, microvessels, and nerves form the basis for acupoints as the reaction sites of acupuncture; acupuncture can cause the deformation of collagen fibers and activate TRPV channels on mast cells membrane, so as to stimulate mast cells to release bioactive substances and activate nerve receptors to generate analgesic effect; system biology models are set up to explain the quantitative process of information initiation and transmission at acupuncture points, and indicate that the acupuncture effect depends on the local mast cells density. In a conclusion, this review will give a scientific explanation of acupuncture analgesia from the material basis of acupoints, the local initiation, and afferent biological mechanism.
Initial In-Brace Correction: Can the Evaluation of Cobb Angle Be the Only Parameter Predictive of the Outcome of Brace Treatment in Patients with Adolescent Idiopathic Scoliosis?
Authors: Aulisa Angelo Gabriele , Galli Marco , Giordano Marco , Falciglia Francesco , Careri Silvia , Toniolo Renato Maria
Published: March 2022

Background: Patients with a better initial in-brace correction could show a higher probability of a successful outcome. However, no one has investigated whether parameters can affect the outcomes. The aim of this retrospective study was to evaluate if the initial correction rate (ICR) could be predictive of the bracing outcome and to determine the role of some mechanical and biological parameters in ICR. Methods: The study population comprised 449 patients who met the inclusion criteria. Curve correction > 10° Cobb defines brace treatment success. Success and failure groups were compared in terms of the Risser sign, initial Cobb angle, initial Perdriolle value and ICR. Results: ICR significantly correlates with initial Perdriolle. The success group had a significantly lower value of Pedriolle and initial Cobb angles, Risser sign and ICR than the failure group. The ICR and lower Risser were significantly associated with the brace treatment outcome. This seems particularly suitable for positivity prediction (Predicting value VP+: 87%). Conclusions: This study confirms that immediate in-brace correction can foretell the brace treatment outcome. Patients with a low Risser sign and a high rate of in-brace correction showed a bracing success of 87%. A correlation between rotation and in-brace correction confirms that rotation is among the parameters that influence the deformed spine reaction to corrective actions the most.
Recurrence of Equinus Foot in Cerebral Palsy following Its Correction—A Meta-Analysis
Authors: Horsch Axel , Klotz Matthias Claus Michael , Platzer Hadrian , Seide Svenja Elisabeth , Ghandour Maher
Published: March 2022

Background: Recurrence in cerebral palsy (CP) patients who have undergone operative or non-operative correction varies greatly from one study to another. Therefore, we conducted this meta-analysis to determine the pooled rate of equinus recurrence following its correction either surgically or non-surgically. Methods: Nine electronic databases were searched from inception to 6 May 2021, and the search was updated on 13 August 2021. We included all studies that reported the recurrence rate of equinus following its correction among CP patients. The primary outcome was recurrence, where data were reported as a pooled event (PE) rate and its corresponding 95% confidence interval (CI). We used the Cochrane’s risk of bias (RoB-II) tool and ROBINS-I tool to assess the quality of included randomized and non-randomized trials, respectively. We conducted subgroup analyses to identify the sources of heterogeneity. Results: The overall rate of recurrence was 0.15 (95% CI: 0.05–0.18; I2 = 88%; p < 0.01). Subgroup analyses indicated that the laterality of CP, study design, and intervention type were significant contributors to heterogeneity. The recurrence rate of equinus differed among interventions; it was highest in the multilevel surgery group (PE = 0.27; 95% CI: 0.19–0.38) and lowest in the Ilizarov procedure group (PE = 0.10; 95% CI: 0.04–0.24). Twelve studies had a low risk of bias, eight had a moderate risk, and nine had a serious risk of bias. Conclusion: The recurrence of equinus following its correction, either surgically or non-surgically, in CP patients is notably high. However, due to the poor quality of available evidence, our findings should be interpreted with caution. Future studies are still warranted to determine the actual risk of equinus recurrence in CP.
Short Versions of the Arabic Psychosocial Impact of Dental Aesthetics Questionnaire for Yemeni Adolescents: Cross-Sectional Derivation and Validation
Authors: Alsanabani Amal A. M. , Yusof Zamros Yuzadi Mohd , Wan Hassan Wan Nurazreena , Aldhorae Khalid , Alyamani Helmi A.
Published: March 2022

Objectives: To shorten the 24-item Arabic Psychosocial Impact of Dental Aesthetics Questionnaire (PIDAQ(A)) for adolescents in Yemen. Material and methods: Two shortening methods derived six-item and nine-item versions: the item impact method selected items with the highest impact scores as rated by 30 participants in each subscale; and the regression method was applied using data of 385 participants from the PIDAQ(A) validity study, with the total PIDAQ(A) score as the dependent variable, and its individual items as the independent variables. The four derived versions were assessed for validity and reliability. Results: The means of the six-item and nine-item short versions of both methods were close. Cronbach’s alpha values extended from 0.90 to 0.92 (intra-class correlations = 0.85–0.88). In criterion validity, strong significant correlations were detected between scores of all short versions and the 24-item PIDAQ(A) score (0.96–0.98; p < 0.001). Construct validity displayed significant associations among all short versions and self-perceived dental appearance rank and self-perceived need for orthodontic braces rank (p < 0.05). Mean scores of all short versions were significantly different between adolescents with severe malocclusion and those with slight malocclusion in discriminant validity tests. In conclusion, all PIDAQ(A) short versions are valid and reliable.
Top-Down Formulation of Goethite Nanosuspensions for the Production of Transparent, Inorganic Glass Coatings
Authors: Peppersack Christoph , Wermbter Karsten , Kwade Arno , Garnweitner Georg , Breitung-Faes Sandra
Published: March 2022

This study presents a simple but effective process route for the production of transparent coatings on glass substrates from inorganic pigment goethite. For this purpose, coating suspensions were prepared by wet milling with a stirred media mill. A water/ethanol mixture was used as the liquid medium to take advantage of the resulting low surface tension for the coating process. In this manner, stable suspensions with particles of down to 50 nm in size were obtained, which already showed a significant increase in transparency. With regard to grinding characteristics, particularly low stress energies proved to be energetically reasonable. The coating step was performed by wet film deposition, achieving coating thicknesses in a range of 0.5–2.5 µm via dip coating. Highly transparent coatings were obtained by applying small particles of 50 nm, which exhibited a significantly lower scattering loss of light (≈3%) in comparison to particles of around 300 nm (70–80%). Additionally, the film color could be adjusted through a variation of the drying temperature due to a conversion of goethite to hematite by dehydration. Since transparency was not affected, this provides an easy-to-implement process adaptation for controlling coating colors.
Electrical Transport Measurements on Layered La(O,F)BiS2 under Extremely High Pressure
Authors: Matsumoto Ryo , Yamamoto Sayaka , Nemoto Yoshihiro , Nishimiya Yuki , Takano Yoshihiko
Published: March 2022

Layered La(O,F)BiS2 exhibits drastic enhancements of the superconducting transition temperature (Tc) under high pressure among the BiS2-based superconducting family. However, the high-pressure application beyond a high-Tc phase of the monoclinic structure has not been conducted. In this study, the electrical transport properties in La(O,F)BiS2 single crystal are measured under high pressures up to 83 GPa. An insulating phase without superconductivity is observed under a higher-pressure region above 16 GPa. Moreover, the sample exhibits metallicity and superconductivity above 60 GPa. The newly observed hidden semiconducting phase and reentrant superconductivity have attracted much attention in BiS2-based compounds.
Gypsum Seeding to Prevent Scaling
Authors: Chagwedera Taona Malvin , Chivavava Jemitias , Lewis Alison Emslie
Published: March 2022

Eutectic freeze crystallization (EFC) is a novel separation technique that can be applied to treat brine solutions such as reverse osmosis retentates. These are often a mixture of different inorganic solutes. The treatment of calcium sulphate-rich brines using EFC often results in gypsum crystallization before any other species. This results in gypsum scaling on the cooled surfaces of the crystallizer, which is undesirable as it retards heat transfer rates and hence reduces the yield of other products. The aim of this study was to investigate and understand gypsum crystallization and gypsum scaling in the presence of gypsum seeds. Synthetic brine solutions were used in this research because they allowed an in-depth understanding of the gypsum bulk crystallization process and scaling tendency without the complexity of industrial brines. A cooled, U-shaped stainless-steel tube suspended in the saturated solution was employed as the scaling surface. This was because a tube-shaped surface enabled the introduction of a constant temperature cold surface in the saturated solution and most industrial EFC crystallizers are constructed from stainless steel. Gypsum seeding was effective in decreasing the mass of scale formed on the heat transfer surface. The most effective seed loading was 0.25 g/L, which reduced scale growth rate by 43%. Importantly, this seed loading is six times the theoretical critical seed loading. The seeding strategy also increased the gypsum crystallization kinetics in the bulk solution, which resulted in an increase in the mass of gypsum product. These findings are relevant for the operability and control of EFC processes, which suffer from scaling problems. By using an appropriate seeding strategy, two problems can be alleviated. Firstly, scaling on the heat transfer surface is minimised and, secondly, seeding increases the crystallization kinetics in the bulk solution, which is advantageous for product yield and recovery. It was also recommended that the use of silica as a seed material to prevent gypsum scaling should be investigated in future studies.
A Rare Case of Plasmablastic Lymphoma in a Patient with HIV and SARS-CoV-2 Infections
Authors: Campeanu Adriana Teodora , Dumea Elena , Rus Mihaela , Fodor Claudia , Ionescu Anita Cristina , Mocanu Elena , Botnarciuc Mihaela , Dumitru Irina Magdalena
Published: March 2022

Lesions commonly associated with HIV infection include oral candidiasis, herpes simplex infection, oral Kaposi’s sarcoma, hairy leukoplakia, periodontal diseases (linear gingival erythema and necrotizing ulcerative periodontitis), xerostomia, human papillomavirus-associated warts, aphthous ulcers, non-Hodgkin’s lymphoma, histoplasmosis, carcinoma, exfoliative cheilitis, and HIV salivary gland disease. Non-Hodgkin’s lymphoma (NHL) is the most common cancer in people living with HIV (PLWH), and the incidence is increased for aggressive B-cell NHL. Plasmablastic lymphoma (PbL) is a rare and aggressive B-cell malignancy that is often unresponsive to chemotherapy and usually has a poor prognosis. We hereby present the case of a patient with a recent history of COVID-19 infection who was diagnosed with HIV and NHL, with manifestations in the oral cavity and a favorable evolution after the introduction of antiviral therapy, specific chemotherapy, and radiotherapy. Dental expertise is necessary for the appropriate management of oral manifestations of HIV infection or AIDS, and lymphoma should be included in the differential diagnosis of any oral lesions.
Immunotherapy in Gastric Cancer
Authors: Högner Anica , Moehler Markus
Published: March 2022

Immune checkpoint inhibition is a new standard of targeted therapy in the treatment of advanced or metastatic gastric cancer (GC) and is represented in various combinations with and without chemotherapy in every therapy line within clinical trials. In advanced adenocarcinoma of GC, gastroesophageal junction cancer (GEJC) and esophageal cancer (EC), the combination of nivolumab and chemotherapy in first-line therapy improves overall survival (OS) in PD-L1 (programmed cell death protein 1)-positive patients with approval in Europe (PD-L1 CPS (combined positivity score) ≥ 5), USA and Taiwan (CHECKMATE-649) and pembrolizumab plus chemotherapy for GEJC and EC in Europe (CPS ≥ 10) and the USA (KEYNOTE-590). Furthermore, pembrolizumab plus trastuzumab and chemotherapy show clear benefits in OS and are approved as first-line treatment of Her2 (human epidermal growth factor receptor-2)-positive tumors in the USA (KEYNOTE-811). Nivolumab demonstrates superior OS regardless of PD-L1 expression in third-line therapy with approval in Japan (ATTRACTION-02) and pembrolizumab prolonged the duration of response in PD-L1 positive patients with approval in the USA in PD-L1 CPS ≥ 1 patients (KEYNOTE-059). This review reflects the rationale and current results of phase II and III clinical trials investigating various immune checkpoint inhibitors targeting PD-L1/1 and CTLA (anticytotoxic T-lymphocyte-associated antigen)-4 in combination with and without chemotherapy and Her2-targeted therapy in GC.
Advances in Diagnosis and Pathophysiology of Microvascular Dysfunction
Authors: Jan Yih-Kuen
Published: March 2022

Microcirculation is the system that brings oxygen and nutrients to local cells and removes metabolic wastes [...]
Transfer Entropy of West Pacific Earthquakes to Inner Van Allen Belt Electron Bursts
Authors: Fidani Cristiano
Published: March 2022

Lithosphere-ionosphere non-linear interactions create a complex system where links between different phenomena can remain hidden. The statistical correlation between West Pacific strong earthquakes and high-energy electron bursts escaping trapped conditions was demonstrated in past works. Here, it is investigated from the point of view of information. Starting from the conditional probability statistical model, which was deduced from the correlation, the Shannon entropy, the joint entropy, and the conditional entropy are calculated. Time-delayed mutual information and transfer entropy have also been calculated analytically here for binary events: by including correlations between consecutive earthquake events, and between consecutive earthquakes and electron bursts. These quantities have been evaluated for the complex dynamical system of lithosphere-ionosphere; although the expressions calculated by probabilities resulted in being valid for each pair of binary events. Peaks occurred for the same time delay as in the correlations, Δt = 1.5–3.5 h, and as well as for a new time delay, Δt = −58.5–−56.5 h, for the transfer entropy; this last is linked to EQ self-correlations from the analysis. Even if the low number of self-correlated EQs makes this second peak insignificant in this case, it is of interest to separate the non-linear contribution of the transfer entropy of binary events in the study of a complex system.
CAD Approach to Control High-Temperature Processes in SiC Technology
Authors: Kubiak Andrzej , Lisik Zbigniew , Raj Ewa
Published: March 2022

Manufacturing silicon carbide semiconductor devices may require high-temperature treatment in closed graphite reactors. This makes temperature control of processed SiC substrates difficult, since their temperature cannot be measured directly. As the monitoring of the SiC sample temperature is critically important for proper process flow, an indirect method involving the use of the CAD approach has been developed. A numerical model of a furnace reactor was created on the basis of the commercial ANSYS package, allowing for the simulation of thermal fields under given heat-dissipation conditions in the modeled area and in the presence of gaseous and liquid media participating in heat exchange and transport. Obtained simulation results remain very consistent with the reference temperature measurements of selected areas of the reactor. The model acts as an accurate tool for temperature distribution verification during the high-temperature annealing of and diffusion of dopants for silicon carbide.
Implementation of Transition Metal Phosphides as Pt-Free Catalysts for PEM Water Electrolysis
Authors: Brito João , Restivo João , Sousa Juliana P. S. , Spera Natalia C. M. , Falcão D. S. , Rocha Amadeu , Pinto A. M. F. R. , Pereira Manuel Fernando R. , Soares Olívia Salomé G. P.
Published: March 2022

Proton Exchange Membrane (PEM) water electrolysis (WE) produces H2 with a high degree of purity, requiring only water and energy. If the energy is provided from renewable energy sources, it releases “Green H2”, a CO2-free H2. PEMWE uses expensive and rare noble metal catalysts, which hinder their use at a large industrial scale. In this work, the electrocatalytic properties of Transition Metal Phosphides (TMP) catalysts supported on Carbon Black (CB) for Hydrogen Evolution Reaction (HER) were investigated as an alternative to Platinum Group Metals. The physico-chemical properties and catalytic performance of the synthesized catalysts were characterized. In the ex situ experiments, the 25% FeP/CB, 50% FeP/CB and 50% CoP/CB with overpotentials of −156.0, −165.9 and −158.5 mV for a current density of 100 mA cm−2 showed the best catalytic properties, thereby progressing to the PEMWE tests. In those tests, the 50% FeP/CB required an overpotential of 252 mV for a current density of 10 mA cm−2, quite close to the 220 mV of the Pt catalyst. This work provides a proper approach to the synthesis and characterization of TMP supported on carbon materials for the HER, paving the way for further research in order to replace the currently used PGM in PEMWE.
Study on Overburden Movement and Fissure Evolution Law of Protective Layer Mining in Shallow Coal Seam
Authors: Li Li , Wu Guiyi , Liu Qinzhi
Published: March 2022

This study aims to effectively solve the problem of the destruction of the coal roof and floor overlying rock after mining the protective layer, and determines whether the gas in the protected layer can be effectively released. We do this based on the engineering background of the Weng’an Coal Mine; research and analysis of the movement of the roof and floor overlying rocks; the evolution of cracks and the pressure relief characteristics of the protected layer after mining the protective layer; and through theoretical analysis and similar simulation experiments. Through numerical simulation, it was found that the protected layer was depressurized due to the mining of the protective layer, and the decompression rate of the protected layer was 0.2–0.8. In addition, the overall expansion rate of the protected layer was greater than the requirements of the “Detailed Rules for Prevention and Control of Coal and Gas Outbursts” for coal mines, and the investigation of the residual gas pressure and content of the protected layer revealed that the protected coal seam had been mined in the upper protective layer coal face. The gas pressure dropped to 50.7% of the original coal seam gas pressure, the rate of decrease was 49.3%, the residual gas content dropped by 68.67%, and the gas concentration in the return airway was 0.31% on average, meeting the national regulations that require its value to be less than or equal to 1%. The study comprehensively demonstrates that mining of the protective layer is beneficial to the release of gas from the protected layer, and provides a practical reference for coal and gas outbursts in mines.
Flexible Matrix of Controllers for Real Time Parallel Control
Authors: Chaber Patryk , Wojtulewicz Andrzej
Published: March 2022

This work aims to develop a novel system, including software and hardware, to perform independent control tasks in a genuine parallel manner. Currently, to control processes with various sampling periods, distributed control systems are most commonly utilized. The main goal of this system is to propose an alternative solution, which allows simultaneous control of both fast and slow processes. The presented approach utilizes FPGA (Field Programmable Gate Array) with Nios II processor (Intel Soft Processor Series) to implement and maintain instances of independent controllers. Instances can implement FDMC (Fast Dynamic Matrix Control) and PID (Proportional-Integral-Derivative) control algorithms with various sampling times. The FPGA-based design allows for true independence of controllers’ execution both from one another and the managing processor. Also, pure parallel execution allows for implementing slow and fast controllers in the same device. The complete flexible system with a matrix of controllers working in parallel in real-time was tested with both simulated and actual control processes (servomotor), yielding the same results as fully simulated experiments.