bluuebunny/crossref_metadata_2025 huggingface dataset:
Normothermic ex vivo lung perfusion using a developed solution followed by orthotopic left lung transplantation (experimental study)
Authors: Pashkov I. V. , Gautier S. V. , Bogdanov V. K. , Oleshkevich D. O. , Bondarenko D. M. , Mozheiko N. P. , Bunenkov N. S. , Grudinin N. V.
Published: July 2023

The continued unavailability of adequate organs for transplantation to meet the existing demand has resulted in a major challenge in transplantology. This is especially felt in lung transplantation (LTx). LTx is the only effective method of treatment for patients with end-stage lung diseases. Normothermic ex vivo lung perfusion (EVLP) has been proposed to increase the number of donor organs suitable for transplant – EVLP has proven itself in a number of clinical trials. The ability to restore suboptimal donor lungs, previously considered unsuitable for transplantation, can improve organ functionality, and thus increase the number of lung transplants. However, widespread implementation of ex vivo perfusion is associated with high financial costs for consumables and perfusate.

Objective: to test the developed solution on an ex vivo lung perfusion model, followed by orthotopic LT under experimental conditions.

Materials and methods. The experiment included lung explantation stages, static hypothermic storage, EVLP and orthotopic left LTx. Perfusion was performed in a closed perfusion system. We used our own made human albumin-based perfusion solution as perfusate. Perfusion lasted for 2 hours, and evaluation was carried out every 30 minutes. In all cases, static hypothermic storage after perfusion lasted for 4 hours. The orthotopic single-lung transplantation procedure was performed using assisted circulation, supplemented by membrane oxygenation. Postoperative follow-up was 2 hours, after which the experimental animal was euthanized.

Results. Respiratory index before lung explantation was 310 ± 40 mmHg. The PaO2/FiO2 ratio had positive growth dynamics throughout the entire EVLP procedure. Oxygenation index was 437 ± 25 mm Hg after 120 minutes of perfusion. Throughout the entire EVLP procedure, there was a steady decrease in pulmonary vascular resistance (PVR). Initial PVR was 300 ± 100 dyn×s/cm5; throughout the EVLP, PVR tended to fall, reaching 38,5 ± 12 dyn×s/cm5 at the end of perfusion.

Conclusion. A safe and effective EVLP using our perfusate is possible. The developed orthotopic left lung transplantation protocol under circulatory support conditions, supplemented by membrane oxygenation, showed it is efficient and reliable.
Difusão de política na prática: o caso da regulação brasileira de medicamentos
Authors: Sizukusa Letícia Oyamada , Fontoura Yuna , Krieger Morgana G. Martins
Published: July 2023

Resumo O presente estudo busca contribuir para a melhor compreensão de processos de difusão de política com base em arenas transnacionais - mais especificamente, do processo de difusão que influenciou a regulação das Boas Práticas de Fabricação (BPF) de medicamentos no Brasil num contexto envolto por atores internacionais. Por meio de pesquisa qualitativa, analisamos o processo de adesão da Agência Nacional de Vigilância Sanitária (Anvisa) ao arranjo de cooperação Pharmaceutical Inspection Co-operation Scheme (PIC/S), iniciado em 2010 e alcançado em 2021. Foi identificado um processo influenciado por 2 constelações de difusão, motivado por interesses da agência nacional em manter sua relevância e por atores que integram o Sistema Nacional de Vigilância Sanitária, no qual o modelo de equivalência e convergência regulatória do PIC/S se mostrou fundamental para a adaptação da referência internacional em nível nacional, mantendo o sistema em funcionamento. Tal processo de difusão de política ficou mais relevante nos últimos anos por ampliar a convergência regulatória e, potencialmente, tornar mais eficiente a avaliação das BPF de medicamentos pelas diversas autoridades sanitárias.
Policy diffusion in practice: the case of Brazilian medicinal products regulation
Authors: Sizukusa Letícia Oyamada , Fontoura Yuna , Krieger Morgana G. Martins
Published: July 2023

Abstract The present study seeks to contribute to a better understanding of policy diffusion processes, more specifically, of the diffusion process from a transnational arena that influenced the regulation of Good Manufacturing Practices (GMP) for medicinal products in Brazil in a context surrounded by international authorities. By conducting qualitative research, we analyzed the process of adhesion of the Brazilian Health Regulatory Agency (Anvisa) to the Pharmaceutical Inspection Co-operation Scheme (PIC/S), initiated in 2010 and achieved in 2021. A process influenced by two constellations of diffusion was identified, motivated by the national agency’s interests in maintaining its relevance and by actors that make up the National Sanitary Surveillance System, in which the PIC/S model of regulatory equivalence and convergence proved to be fundamental for the adaptation of the international reference to the national level, keeping the national system functioning. Such a policy diffusion process became even more relevant in the past years due to the expansion of regulatory convergence and potentially making the various health authorities’ GMP assessment of medicinal products more efficient.
Quando em Roma, faça como os romanos: um estudo de caso da Odebrecht e o contínuo da destrutividade
Authors: Coelho Caio César
Published: July 2023

Resumo Esta pesquisa conduz um estudo de caso de uma empresa de construção pesada Odebrecht para responder à questão: Como um indivíduo racionaliza o crime em uma organização corrupta? Este estudo é baseado nos conceitos de organização corrupta, contínuo da destrutividade, desengajamento moral e racionalização. Nós analisamos quatro livros que são artefatos da cultura da Odebrecht e vídeos de 49 executivos que colaboraram na investigação da Lava Jato. Os resultados descrevem os caminhos que os funcionários trilham dentro da organização, adquirindo seus sistemas de valores, crenças e pressupostos. Estes caminhos levam a racionalização da corrupção. Este estudo de caso mostra que o contínuo da destrutividade começa quando empregados encontram comportamentos antiéticos dentro da organização e que os mecanismos de racionalização se modificam com o tempo dentro da cultura corrupta. A qualquer momento executivos podem pedir demissão ou denunciar; no entanto, com o tempo se torna difícil exercer qualquer uma dessas opções. Ao aplicar e refinar o contínuo, esta pesquisa prove um entendimento sobre como desengajamento moral e racionalização incentivam funcionários a seguir adiante no contínuo.
Difusão da Lei Geral da Micro e Pequena Empresa em municípios paulistas
Authors: Carvalho Grégory dos Passos , Coêlho Denilson Bandeira
Published: July 2023

Resumo Este artigo tem como objeto de análise a implantação da Lei Complementar nº 123, de 2006 (LC nº 123/2006), que instituiu o Estatuto Nacional da Microempresa e da Empresa de Pequeno Porte (MPE), por municípios paulistas. Utilizou-se o referencial teórico de difusão de políticas públicas tendo como objetivo estudar seu processo de adoção, observando os diferentes contextos e momentos de coerção previstos na legislação federal. Com base nas discussões teóricas relacionadas com o mecanismo de difusão chamado de coerção, respondeu-se a seguinte pergunta: qual é a influência do mecanismo de coerção vertical na difusão da Lei Geral da MPE nos municípios paulistas? A técnica “análise de sobrevivência” foi aplicada para identificar fatores explicativos da difusão da política, considerando variáveis de desenho institucional, políticas e partidárias, efeito vizinhança, necessidade da política, organizações/atores sociais e fatores estruturais. Os resultados demonstram que, conforme varia o desenho institucional de coerção, mudam os fatores explicativos da difusão dessa política na ponta, de modo que os aspectos locais, incluindo atores sociais, fatores regionais e questões estruturais, importam para a difusão, variando conforme o contexto coercitivo.
Climate financing: institutional effectiveness of the national fund on climate change
Authors: Lopes Ariane Cristina Cordeiro Gazzi , Albuquerque Andrei Aparecido de
Published: July 2023

Abstract There is broad scientific consensus about the climate emergency. Governments have intensified mitigation and adaptation actions by conducting climate finance through national climate funds. In 2009, the National Fund on Climate Change (FNMC) was established in Brazil. Analyzes on climate finance have intensified after 2020, and there are few empirical studies on these funds. This article contributes to this gap by analyzing the institutional effectiveness of the FNMC through desk research in the period from 2009 to 2020, considering 21 indicators distributed in five dimensions. This study identifies the main challenges to the FNMC’s institutional effectiveness, recognizing its importance in helping Brazil meet its commitment to international climate goals by reducing greenhouse gases (GHG), strengthening research, and increasing investments in climate change projects. The results reveal great fragility in the FNMC’s institutional effectiveness. None of the five dimensions analyzed had all indicators satisfied, and resource mobilization and sustainability were identified as the main challenges to the FNMC’s institutional effectiveness.
Perfil dos convênios celebrados pelo Ministério da Justiça e Segurança Pública com entes subnacionais no Brasil (2008-2022)
Authors: Lui Lizandro , Sales Eric Rodrigues de
Published: July 2023

Resumo O presente artigo tem como objetivo analisar, com base na discussão sobre instrumentos de políticas públicas e nos estudos sobre as políticas de segurança pública no Brasil, o perfil das transferências voluntárias de recursos orçamentários da União celebradas por meio do Ministério da Justiça e Segurança Pública (MJSP) com os entes subnacionais, no período de 2008 a 2022. Trata-se de uma pesquisa documental e de uma análise de conteúdo sobre dados de acesso público extraídos da Plataforma +Brasil. Identificou-se que os convênios dialogam com o paradigma da segurança pública voltada à prevenção da violência, que os estados das regiões Sudeste e Sul figuram como os maiores beneficiados das transferências voluntárias do MJSP e que o Congresso Nacional passou a ser o principal financiador dos convênios ao longo do tempo. O estudo conclui que é preciso esforços por parte do Governo Federal com vistas à distribuição de recursos de forma equânime pelo território.
Genetic progress in 53 years of the Peach Breeding Program of Embrapa: Fresh market cultivars
Authors: Corrêa Elisia Rodrigues , Nardino Maicon , Raseira Maria do Carmo Bassols , Real Isadora Moreira da Luz , Barros Willian Silva , Franzon Rodrigo Cezar
Published: July 2023

Abstract A peach breeding program started in 1963, at the Experiment Station of Pelotas, nowadays Embrapa Clima Temperado, whose primary aim was developing cultivars adapted to mild winter conditions. Its first priority was to obtain cultivars of canning type fruits, and over the years, the fresh market cultivars acquired equal importance. This article analyzes the data of 84 fresh market cultivars, obtained in Pelotas, from 1964 to 2017, focusing on the following parameters: time of ripening, fruit development period, average fruit mass, number of fruits per tree, productivity per plant and total soluble solids. First, the data were tabulated, divided into two periods (1964-1984 and 1985-2017), and the descriptive statistical analysis was performed, followed by an analysis via mixed models and estimates of genetic progress via meta-analysis. The main results revealed a reduced fruit development period of the fresh market peaches belonging to that program. It was also observed a significant spread of the time of ripening (end of September or beginning of October until January), with a slight tendency for earliness. Genetic gain was observed for yield corresponding to 1.17 and 2.25% per year, for 1964-1984 and 1985-2017, respectively.
Coefficient of repeatability, stability,and adaptability estimates for Psidium guajava S1 progenies via mixed models
Authors: Ambrósio Moisés , Viana Alexandre Pio , Cavalcante Natan Ramos , Preisigke Sandra da Costa , Santana Josefa Grasiela Silva , Crevelari Jocarla Ambrosim
Published: July 2023

Abstract This study intends to estimate the coefficient of repeatability for traits relating to yield, so as to infer what is the minimum number of evaluations required to identify superior genotypes; to verify adaptability and stability; to predict genetic gains; and to select the best families and in S1 progenies of Psidium guajava. Families were obtained by means of self-fertilization of superior genotypes (full sibs) from the guava tree genetic breeding program of the UENF. The experiment was implemented at the Experimental Station of Ilha Barra do Pomba, in the municipality of Itaocara-RJ. In this case, an experimental randomized block design was used with 18 inbred families, three replicates, and ten plants per plot. Inbred families were evaluated in three harvests regarding fruit weight, number of fruits per plant, and total yield. It was then conducted an analysis of repeatability, adaptability, and stability of genotypes by means of the Selegen/REML/BLUP program. Repeatability values show that the performance of genotypes is constant among measurements, suggesting the need to perform only three evaluations for the selection of superior genotypes. There was agreement on the choice of families 1, 4, 6, and 12 as the most yielded, adaptable, and stable for the traits evaluated.
Effect of fruits maturation stage and vegetal regulators in the physiological quality of seeds sweet passion fruit cv. BRS Mel do Cerrado
Authors: Silveira Flávia Aparecida da , Faleiro Fábio Gelape , Oliveira Jamile da Silva , Junqueira Nilton Tadeu Vilela , Araújo Neto José Eufrosino de , Damacena Geisybell Lopes
Published: July 2023

Abstract This study aimed to evaluate the effect of fruit maturation stages and pre-germination treatments with plant regulators on the physiological quality of seeds of cv. BRS Mel do Cerrado. Two experiments were carried out, germination and emergence, installed in a completely randomized design in a 3 x 2 factorial arrangement, with three stages maturation (1-partially ripe fruits; 2-fully ripe fruits and 3-senescent fruits) and two pre-germination treatments (1. [GA4+7 +N-(phenylmethyl)-aminopurine (300 ppm)] and 2. distilled water), forming six treatments with four replications of 50 seeds. The evaluated characteristics were germination percentage at 14 and 35 days, germination speed index (GSI), emergence percentage and emergence speed index (ESI). Germination at 14 days, GSI and seedling emergence were not affected by the maturation stage, however germination and GSI were higher when the plant regulator was used. The final percentage of germination was higher in seeds taken from partially ripe fruits and treated with regulators and a higher ESI was observed for seeds from senescent fruits and treated with regulator. Seeds treated with plant regulators are more vigorous and have a higher rate of seedling emergence.
Carbohydrate, flavonoid, anthocyanin,total phenol, chlorophyll and mineral (K+) content development of wax apple fruit as affected by CPPU and NAA using swabbing technology
Authors: Hossain Ahmed Bin Mohamed Sharif , Alsaif Adel Mohamed , Rudayni Hassan Ahmed , Al-Hashimi Abdulrahman , Aleissa Mohammad Saad , Taha Rosna Mat
Published: July 2023

Abstract The study was conducted to investigate the effect of CPPU(2-Chloro-4-pyridyl-phenylurea) and NAA (Naphthaleneacetic acid) on the pigments, total phenol, flavonoid and mineral (K+) content in wax apple fruit. CPPU concentrations were 10, 15 and 20ppm (Expt.1) and 6, 12, 18ppm NAA were applied coming after swabbing technique (Expt.2). In Expt.1, the lowest fruit weight, fruit size and chlorophyll content was observed in control treatment. However, the highest fruit weight, fruit size and chlorophyll (SPAD) content was found in 15ppm CPPU. The most effective concentration was 15ppm CPPU for the earlier fruit maturity (color development) compared to other concentrations. Moreover, flavonoid, fructose, inverted sugar, total phenol and K+ were higher in 15ppm CPPU than control, 10 and 20ppm CPPU. In addition, anthocyanin was found increasing trend while developing the fruit maturity represented by color development. In Expt.2, chlorophyll was higher in 15ppm CPPU than control, 6, 18ppm NAA. Furthermore, flavonoid, fructose, inverted sugar, total phenol and K+ content were higher in 12ppm NAA than control, 6 and 18ppm NAA. Besides, the maximum anthocyanin was found in 12ppm NAA. Finally it seemed that 15ppm CPPU and 12ppm NAA were the best concentration for fruit growth and biochemical contents development in wax apple.
Postharvest longevity of Fremont IAC 543 mandarin
Authors: Pacheco Camilla de Andrade , Azevedo Fernando Alves de , Silva Danilo José da , Shimizu Gabriel Danilo , Conceição Patrícia Marluci da
Published: July 2023

Abstract The planting of new types of table citrus is an interesting alternative for citrus growers in the state of Paraná, as this domestic market continues to grow and needs innovation. Thus, the aim of this study was to evaluate the acceptance and storage of a new hybrid for the region of Londrina / PR, Fremont IAC 543 mandarin (Citrus clementina hort. ex Tan x C. reticulataBlanco). Treatments were: storage of fruits with and without wax coating at room temperature [21 ± 2 °C and relative humidity (RH) = 60 ± 5%] for up to 36 days. Sensory (acceptance test and purchase intention) and physicochemical analyses [weight, juice yield, acidity, soluble solids and soluble solids/titratable acidity (ratio)] were performed before and after storage. Physicochemical data were submitted to analysis of variance and regression, while sensory analysis results were explored through descriptive statistics and principal components analysis using the R statistical software. It could be concluded that Fremont IAC 543 mandarin can be stored with wax coating and at room temperature without losing its quality characteristics for a period of up to 18 days.
“Decifra-me ou te devoro”, o enigma da uberização: análise do trabalho e da saúde
Authors: Souza Diego de Oliveira
Published: July 2023

Resumo: Esta pesquisa buscou analisar a relação trabalho-saúde no bojo da uberização, tomando a perspectiva das próprias plataformas como objeto da crítica marxista. Constatou-se que essas empresas propagam a promessa do “ganhe dinheiro”, expressando uma série de fetichismos que está na base da construção da falácia da autogestão e do empreendedorismo. No que diz respeito à saúde, reproduzem, de forma caricata, velhos modelos de intervenção na saúde dos trabalhadores, repetindo a história como farsa.
Lycopene enhances bone neoformation in calvaria bone defects of ovariectomized rats
Authors: Ricardo Vitória , Sousa Luiz Gustavo de , Regalo Isabela Hallak , Pitol Dimitrius Leonardo , Bombonato-Prado Karina Fittipaldi , Regalo Simone Cecilio Hallak , Siessere Selma
Published: July 2023

Abstract Osteoporosis can affect a significant part of the population and fractures are the most common complications associated with this disease, leading to high public health costs. Thus, the prevention of fractures is relevant to individuals with signs and symptoms as well as to the health system. Postmenopausal osteoporosis has been associated with oxidative stress, emphasizing the importance of an efficient defense system to maintain bone health. Lycopene is a carotenoid with antioxidant properties that may stimulate osteoblastogenesis and inhibit osteoclastogenesis. The purpose of this investigation was to analyze the influence of lycopene in the bone neoformation of calvaria defects in ovariectomized rats utilizing the concentration of 45 mg/kg. Wistar Hannover female rats were divided into ovariectomized and sham groups. The ovariectomized animals received 45 mg/kg lycopene (OvxL) or water (Ovx) by daily gavage the day after ovariectomy/sham surgery for 16 weeks. Twelve weeks after ovariectomy, there were performed 5-mm calvaria defects followed by euthanasia after 4 weeks. Samples of bone tissue were collected to perform morphological and morphometrical analysis of the neoformed bone area, and percentage with Software Image J. Morphological evaluation showed mature bone with more osteocytes in the group OVxL when compared to the other groups. The morphometrical analysis demonstrated a significant increase of bone neoformation in the group OvxL (p<0.05). The data obtained suggest that lycopene benefits bone repair in the absence of estrogenic hormones.
Diagnosis and prevalence of probable awake and sleep bruxism in adolescents: an exploratory analysis
Authors: Prado Ivana Meyer , Abreu Lucas Guimarães , Pordeus Isabela Almeida , Amin Maryam , Paiva Saul Martins , Serra-Negra Junia Maria
Published: July 2023

Abstract The aims of this study were to perform an exploratory analysis of probable awake (AB) and sleep bruxism (SB) prevalence using of different diagnosis criteria based on the International Consensus; evaluate the associations between self-report and clinical signs/symptoms in adolescents. Participated in this cross-sectional study 403 adolescents aged 12- to 19-years-old enrolled in public and private schools from Belo Horizonte, Brazil. Parents/caregivers answered a questionnaire about sociodemographic status and adolescents’ health status. Adolescents answered a questionnaire evaluating AB (e.g., grinding and clenching) and SB (e.g., grinding, bracing, and thrusting) activities and frequent headaches. A clinical examination was performed on adolescents to evaluate bruxism clinical signs/symptoms (pain upon palpation on masseter and temporal, linea alba, indentation on the tongue and attrition wear severity). Descriptive statistics and Pearson’s Qui-square test were performed (P≤0.05). Adolescents mean age was 14.3±1.5 years, and 58.1% were female. Self-report of SB was identified in 31% of participants and self-report of AB in 51.6%. Almost all adolescents (99%) presented at least one tooth with attrition wear (98.5% on enamel and 0.5% on dentin), with a mean number of 12.4±5.7 teeth. Depending on the diagnosis criteria, the prevalence of probable SB and AB varied from 0- 99% and 0.2- 99%, respectively. A high inconsistency was found for the prevalence of probable AB and SB in adolescents, which were influenced by the different clinical sings/symptoms used as diagnosis criteria. Frequent headaches and pain upon palpation on masseter and temporal muscle were associated to self-report of AB and SB among adolescents.
Anti-erosion effect of an experimental varnish on eroded dentin
Authors: Monteiro Filho George , Barros Antonia Patricia Oliveira , Fernandes Gabriela Carvalho Santos , Jassé Fernanda Ferreira de Albuquerque , Kuga Milton Carlos , Alencar Cristiane de Melo
Published: July 2023

Abstract This in vitro study evaluated the effect of an experimental varnish containing 20% nano-hydroxyapatite (nHAP) associated with 5% stannous chloride (SnCl2) against erosive-abrasive wear on bovine dentin. Samples of bovine cervical dentin were pre-eroded (0.3% citric acid, pH 2.6 for 10 minutes) and randomized into 4 groups (n=10): Control group - experimental varnish without active ingredient (CG); experimental varnish containing 20% nHAP (nHG); experimental varnish containing 5% SnCl2 (24.800 ppm Sn2+) (SnG); experimental varnish containing 20% nHAP associated with 5% SnCl2 (18.300 ppm Sn2+) (nHSnG). After applying the materials, the erosive-abrasive challenges were performed for five days. Erosive dentin loss and analysis of the pattern of dentinal obliteration were performed by 3D confocal laser microscopy. A one-way ANOVA/Bonferroni test was performed to analyze the data (α=0.05). The SnG and nHSnG experimental groups presented more effectiveness in preventing erosive wear when compared to the other groups (p<0.05). There was no statistically significant difference between the SnG and nHSnG groups (p = 0.731) in tooth structure dentin loss. Regarding the amount of open dentinal tubules, the highest amount of obstructed dentinal tubules was demonstrated in SnG and nHSnG (p < 0.05) when compared to the others. Between SnG and nHSnG there was no significant difference (p = 0.952) in the amount of closed dentinal tubules in the dentin. Experimental varnishes containing 5% SnCl2 associated or not with 20% nHAP showed to be a promising strategy in preventing erosive-abrasive wear of dentin. In addition, nHSnG was able to obliterate dentinal tubules.
Epithelial responses and Candida albicans pathogenicity are enhanced in the presence of oral streptococci
Authors: Martorano-Fernandes Loyse , Brito Arella Cristina Muniz , Araújo Elza Cristina Farias de , Almeida Leopoldina de Fátima Dantas de , Wei Xiao-Qing , Williams David Wynne , Cavalcanti Yuri Wanderley
Published: July 2023

Abstract Experimental models that consider host-pathogen interactions are relevant for improving knowledge about oral candidiasis. The aim of this study was to assess the epithelial immune responses, Candida penetration of cell monolayers, and virulence during mixed species culture infections. Single species cultures of Candida albicans and mixed cultures (C. albicans, Streptococcus mutans, and Streptococcus sanguinis) were used to infect monolayers of HaCaT and FaDu ATCC HTB-43 cells for 12 h. After infection, IL-18 and IL-34 gene expression was measured to assess epithelial cell immune responses, and lactate dehydrogenase (LDH) activity was measured as an indicator of cell damage. Microscopy determined C. albicans morphology and penetration of fungal cells through the keratinocyte monolayer. Monolayers devoid of infection served as controls. Data were analyzed by an ANOVA one-way test followed by Tukey’s post-hoc test (α = 0.05). The results found that IL-18 and IL-34 gene expression and LDH activity were significantly (p < 0.05) upregulated for both cell lines exposed to mixed species cultures compared with C. albicans alone. Candida albicans yeast and hyphae were evident in C. albicans only infections. In contrast, monolayers infected by C. albicans, S. mutans, and S. sanguinis exhibited higher microbial invasion with several hyphal aggregates detected. The presence of streptococci in C. albicans infection enhances the virulence and pathogenicity of the fungus with associated increased immune responses and tissue damage. Extrapolation of these findings to oral infection would indicate the added potential benefit of managing bacterial components of biofilms during treatment.
As ressonâncias da loucura materna nos processos de subjetivação de uma filha
Authors: Barbosa Adilane dos Santos , Jucá Vládia Jamile dos Santos , Torrenté Mônica de Oliveira Nunes de
Published: July 2023

Resumo Este artigo aborda as ressonâncias da loucura materna na subjetivação de uma filha a partir de um estudo de caso. Trabalhou-se com história de vida e foi realizado um diálogo com os processos de subjetivação e práticas de cuidado de si. A análise temática organizou os resultados a partir das seguintes categorias: as ressonâncias da loucura materna nos processos de subjetivação; o cuidado de si como horizonte transformador nos processos de subjetivação; o trabalho de subjetivar um legado transgeracional e a elaboração da mãe como referência de maternidade. Entre os resultados, destacam-se: a presença de afetos ambivalentes, a inconstância da presença física e da disponibilidade psíquica maternas e o desenvolvimento, por parte da entrevistada, de práticas de cuidado destinadas a si e a ao outro. Uma trajetória marcada por violência, questões de raça, gênero e classe, dificuldades sociais e econômicas que intensificaram a condição de sofrimento psíquico.
Identification of volatile compounds in salep (Serapias vomeracea) tubers and effects of harvest time and drying method on composition variation
Authors: Şavşatlı Yusuf
Published: July 2023

ABSTRACT Serapias vomeracea is an economically valuable plant belonging to the Orchidaceae family; particularly, its tubers are consumed as hot drinks. Past studies on S. vomeracea have focused on volatile components only in the aerial parts of this orchid. This study is the first to investigate the volatile compounds present in S. vomeracea tubers, which have a high commercial value. Additionally, we determined the effect of harvest time and drying method on the volatile compound profile. The tubers were harvested on two different days (May 17, 2022, and June 6, 2022) and subjected to four different drying conditions (shade drying, oven drying, shade drying after boiling, and lyophilizer drying). To determine the volatile compound profile, sample extracts were prepared by solid-phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry. Overall, 22 volatile compounds were identified: 3 esters, 3 aldehydes, 3 alcohols, 3 terpenes, 3 alkanes, 3 carboxylic acids, 1 phenol, 1 ketone, 1 lactone, and 1 furan. The results revealed that p-cresol was the main compound. During harvesting and subsequent drying, some compounds were lost, and some new compounds were formed. Moreover, harvest time had quite limited effects on volatile compounds in the lyophilizer method. Cluster analysis revealed that the combination of harvest time and drying was effective in the distribution of volatile compounds in salep powder.
Adaptability and stability of mungbean genotypes in the Mid-North of Mato Grosso, Brazil
Authors: Noleto Mariano Pereira , Menezes Júnior José Ângelo Nogueira de , Olibone Dácio , Gobbi Simone Daneluz , Pivetta Laerte Gustavo , Silva Kaesel Jackson Damasceno e
Published: July 2023

ABSTRACT Evaluation of genotype x environment interactions is essential in plant breeding aimed at adapting crops to new settings. Considering the need for research on the adaptation of mungbean to grain-production regions of Brazil, this study evaluated the agronomic performance, adaptability, and stability of mungbean production in the mid-north of Mato Grosso using different genotypes. Two experiments (with and without fertilizer application) were carried out in Sorriso and Sinop in 2019 and 2020, totaling eight environments. The treatments were 10 mungbean lines and the control cultivar BRSMG Camaleão. In general, significant differences among the treatments were observed in the yield, mass of 100 grains, and value for cultivation. The genotype x environment interactions were significant for yield. On average, the yield was in the range of 734-1305 kg ha-1, the mass of 100 grains was 4.63-6.56 g, and the value for cultivation was 2.08-3.56. Genotypes BRA-08654-1, BRA-000027, BRA-084654-2, and BG3 combined high average yield and mass of 100 grains. These genotypes also showed good adaptability for cultivation in the mid-north of Mato Grosso. The yellow seed-coated genotype BRA-084689 also showed good agronomic performance and adaptability. Genotypes BRA-08654-1, BRA-000027, BRA-084654-2, BRA-084689, and BG3 are promising for further experiments evaluating the value for cultivation and use, the final breeding stage consisting of tests at a national level.
Ecological importance of lepidopteran defoliators on eucalyptus plantations based in faunistic and natural enemy analyses
Authors: Ghiotto T. C. , Barbosa M. C. , Guerreiro J. C. , Prado E. P. , Masson M. V. , Tavares W. S. , Wilcken C. F. , Zanuncio J. C. , Ferreira-Filho P. J.
Published: July 2023

Abstract Areas planted with Eucalyptus urophylla S.T. Blake variety platyphylla F. Muell. (Myrtaceae) expand annually in most regions of Brazil. Many lepidopteran species defoliate this plant, but with damage varying per species. The objective of this study was to identify the pest status of lepidopteran defoliators based in the faunistic analysis of these insects and of their natural enemies on E. urophylla variety platyphylla plantations in a representative producing region of Brazil. Adult moths of lepidopterans and of their natural enemies were captured using a light trap, installed every two weeks, from September 2016 to August 2018. A total of 183, 10, three and 139 lepidopteran species was captured and classified as primary, secondary, without defined importance to eucalypt plants and non-identified with 1,419, seven, 465 and 876 individuals, respectively. Two primary pest species were constants, two accessories and six accidentals and all secondary ones were accidentals. Six primary pest species were common and dominant and four non-dominants. Faunistic indices indicated the main lepidopteran species that should be monitored in pest management programs. Seven hymenopteran species (65 individuals), three dipterans (49 individuals) and two hemipterans (four individuals) were the natural enemies collected using light traps. The monitoring of lepidopteran pests with light traps can contribute to the management and to reduce damage and control costs for these species, besides identifying natural enemies for biological control programs in Eucalyptus plantations.
Brassavola tuberculata Hook.: in vitro growth and ex vitro establishment as a function of the micropropagation system and sucrose
Authors: Soares J. S. , Ramos J. C. M. , Sorgato J. C. , Ribeiro L. M. , Reis L. C.
Published: July 2023

Abstract This study examines the in vitro growth and ex vitro establishment of Brassavola tuberculata in relation to the micropropagation system and sucrose concentration employed in the in vitro culture. A completely randomized experimental design was utilized, employing a 2 x 5 factorial arrangement. The experimental period began with seedlings cultivated in vitro for 180 days, which were subsequently transferred to Murashige and Skoog culture media containing sucrose concentrations of 0, 15, 30, 45, or 60 g L-1. The cultures were subjected to two micropropagation systems: conventional and gas exchange. After 90 days of in vitro cultivation, the plants were evaluated, transplanted into a substrate, and placed in a screened nursery for ex vitro cultivation. After 300 days of ex vitro cultivation, the survival and initial characteristics of the plants were assessed. The micropropagation system allowing gas exchange and sucrose concentrations up to 30 g L-1 enhanced the shoot and root growth of in vitro propagated plants. No noticeable anatomical differences were observed after 90 days of in vitro culture among the different sucrose concentrations and micropropagation systems used. In the ex vitro establishment, irrespective of sucrose concentration, the micropropagation system facilitating gas exchange positively influenced all evaluated characteristics.
Effect of temperature on embryonic development and first exogenous feeding of goldfish Carassius auratus (Linnaeus, 1758)
Authors: Motta J. H. S. , Glória L. S. , Radael M. C. , Mattos D. C. , Cardoso L. D. , Vidal-Júnior M. V.
Published: July 2023

Abstract Goldfish or Kinguio is a widely marketed species worldwide due to the ornamental market. There is some lack of acknowledgment of the production of the species under specific climatic conditions. To evaluate the effect of temperature on embryonic development and the first exogenous feeding of goldfish, an experiment was proposed. Fifteen incubators, organized in five treatments (18, 22, 26, 30, and 34 °C) with three replications each, were used to keep the fertilized goldfish eggs until the first exogenous feeding of the larvae. The main development events were observed to understand the possible effects of these temperatures on embryos and larvae of the species. Temperature influences embryo development and the time of first exogenous feeding of goldfish. The temperature of 34 °C was lethal to the species causing 100% of anomalies in the embryos and larvae. The experiment data allow us to conclude that the species presents a maximum thermal limit during embryogenesis, and these data are important to the aquaculture industry and to understand the effect of climate changes on goldfish. The data obtained in this experiment will assist in the management of invasive species and production of the species (aquaculture).
Amelioration of exosome and mesenchymal stem cells in rats infected with diabetic nephropathy by attenuating early markers and aquaporin-1 expression
Authors: Zahran F. , Nabil A. , Nassr A. , Barakat N.
Published: July 2023

Abstract Diabetic nephropathy (DN) is a prevalent diabetic microvascular condition. It is the leading cause of kidney disease in the advanced stages. There is no currently effective treatment available. This research aimed to investigate the curative potentials of exosomes isolated from mesenchymal stem cells affecting DN. This study was performed on 70 male adult albino rats. Adult rats were randomized into seven groups: Group I: Negative control group, Group II: DN group, Group III: Balanites treated group, Group IV: MSCs treated group, Group V: Exosome treated group, Group VI: Balanites + MSCs treated group and Group VII: Balanites + exosome treated group. Following the trial period, blood and renal tissues were subjected to biochemical, gene expression analyses, and histopathological examinations. Results showed that MDA was substantially increased, whereas TAC was significantly decreased in the kidney in the DN group compared to normal health rats. Undesired elevated values of MDA levels and a decrease in TAC were substantially ameliorated in groups co-administered Balanites aegyptiacae with MSCs or exosomes compared to the DN group. A substantial elevation in TNF-α and substantially diminished concentration of IGF-1 were noticed in DN rats compared to normal health rats. Compared to the DN group, the co-administration of Balanites aegyptiacae with MSCs or exosomes substantially improved the undesirable elevated values of TNF-α and IGF-1. Furthermore, in the DN group, the mRNA expression of Vanin-1, Nephrin, and collagen IV was significantly higher than in normal healthy rats. Compared with DN rats, Vanin-1, Nephrin, and collagen IV Upregulation were substantially reduced in groups co-administered Balanites aegyptiacae with MSCs or exosomes. In DN rats, AQP1 expression was significantly lower than in normal healthy rats. Furthermore, the groups co-administered Balanites aegyptiacae with MSCs or exosomes demonstrated a substantial increase in AQP1 mRNA expression compared to DN rats.
Acute pulmonary embolism as a complication in a young male patient with a left popliteal venous aneurysm
Authors: Contreras-Jiménez Emmanuel , Martínez-Quesada Jose I. , Miranda-Ramirez Montserrat W. , Anaya-Ayala Javier E. , Arzola-Flores Luis H. , Mier y Terán-Ellis Santiago , Cuen-Ojeda Cesar , Hinojosa Carlos A.
Published: July 2023

Abstract Venous aneurysms are rare and have a prevalence of 0.1 to 0.2% in the reported series. Typically, patients do not present any symptoms, but are prone to develop deep venous thrombosis (DVT) and the most feared complication, pulmonary embolism (PE). We present the case of a previously healthy 36-year-old man who presented at the emergency department with tachycardia, dyspnea, and pleuritic pain. A thoracic computed tomography angiography (CTA) confirmed the diagnosis of acute pulmonary embolism. He was treated with systemic thrombolysis and anticoagulation. In the further workup of the cause of the embolism, computed tomography revealed a fusiform dilation of the left popliteal vein measuring 3 by 3 centimeters (cm) with an incomplete filling defect because of thrombus presence. The patient underwent open surgical repair. At one month follow-up, he was asymptomatic, and an ultrasound revealed complete patency of the popliteal vein without dilatation or thrombus.
A rare cause of deep vein thrombosis: inferior vena cava agenesis
Authors: Del Canto Peruyera Pablo , Vallina-Victorero Vázquez Manuel Javier
Published: July 2023

Abstract Inferior vena cava agenesis is a rare condition and is often misdiagnosed. This anomaly is asymptomatic in the majority of cases and is usually diagnosed during imaging tests carried out for other purposes. The most frequent manifestation is deep vein thrombosis (DVT) in lower limbs and anticoagulation therapy is the most frequent treatment option. Other techniques such as thrombolysis and venous bypass are also described. We report two cases diagnosed at our institution during the last year, both of which presented with an episode of DVT. We opted for indefinite anticoagulation therapy and both patients remain asymptomatic, after 1 year of surveillance in the first case and 6 months in the second, with no new episodes of DVT. Although it is not a life-threatening anomaly, it is important to make an appropriate diagnosis and provide treatment to improve the symptoms and quality of life of these patients.
Adequação da estratificação de risco e da profilaxia do tromboembolismo venoso em hospital universitário terciário
Authors: Ramalli Junior Edvaldo Luiz , Dalio Marcelo Bellini , Ribeiro Maurício Serra , Joviliano Edwaldo Edner
Published: July 2023

Resumo Contexto O tromboembolismo venoso (TEV) é a terceira doença cardiovascular mais comum e a principal causa de óbito evitável em pacientes internados. Apesar de diretrizes bem estabelecidos na literatura, a profilaxia dessa afecção ainda é subutilizada. Estudos apontam taxa de adequação da profilaxia próxima de 50% mundialmente. Objetivos Avaliar a adequação da estratificação de risco e da prescrição de medidas de profilaxia do TEV em um hospital universitário terciário. Métodos Estudo transversal observacional realizado através da coleta de dados de prontuário. Foram incluídos pacientes adultos internados em diferentes especialidades e divididos em grupos cirúrgico e clínico. A estratificação de risco de TEV realizada pelos médicos assistentes foi comparada com a realizada pelos médicos pesquisadores, com base nas diretrizes recentes. A prescrição de medidas de profilaxia realizadas pelos médicos assistentes foi comparada com as recomendações das diretrizes, obtendo assim a taxa de adequação da profilaxia. Resultados Foram avaliados 400 pacientes, sendo 169 (42,3%) cirúrgicos e 231 (57,7%) clínicos. A taxa geral de adequação da estratificação foi de 50,8%. Nos grupos cirúrgico e clínico, as taxas de adequação foram respectivamente 39,1% e 59,3% (p < 0,0001). A taxa geral de adequação da profilaxia foi de 71,5%, enquanto no grupo cirúrgico foi de 78,1% e no grupo clínico de 66,7% (p = 0,0137). Conclusões A adequação da estratificação de risco para TEV foi baixa, demonstrando a pouca conscientização dos médicos assistentes sobre esse problema. No entanto, as taxas de adequação da prescrição de medidas de profilaxia estão superiores às de dados globais.
Adequacy of venous thromboembolism risk stratification and prophylaxis in a tertiary university hospital
Authors: Ramalli Junior Edvaldo Luiz , Dalio Marcelo Bellini , Ribeiro Maurício Serra , Joviliano Edwaldo Edner
Published: July 2023

Abstract Background Venous thromboembolism is the third most common cardiovascular disease and the main cause of preventable death in hospitalized patients. Prophylaxis is still underused, despite well-established guidelines in the literature. Studies show a worldwide prophylaxis adequacy rate close to 50%. Objectives To assess the adequacy of risk stratification and prophylactic measures for venous thromboembolism in a tertiary university hospital. Methods A cross-sectional observational study was carried out, collecting data from medical records. Adult patients hospitalized by different specialties were enrolled and divided into surgical and clinical groups. The risk stratification of venous thromboembolism performed by the attending physicians was compared with stratification based on recent guidelines performed by the research physicians. Prophylaxis measures prescribed by the attending physicians were compared with guideline recommendations, thus obtaining the prophylaxis adequacy rate. Results 400 patients were analyzed, 169 (42.3%) surgical and 231 (57.7%) clinical. The overall stratification adequacy rate was 50.8%. Adequacy rates were 39.1% and 59.3% in the surgical and clinical groups respectively (P < 0.0001). The overall prophylaxis adequacy rate was 71.5%, with 78.1% in the surgical group and 66.7% in the clinical group (P=0.0137). Conclusions Risk stratification adequacy is low, demonstrating a low awareness among prescribing physicians of the need for adequate stratification for prescription of prophylaxis. However, the prophylaxis prescription adequacy rates are higher than those in global data.
Influência dos fatores de risco para aterosclerose na distribuição anatômica da doença arterial periférica em pacientes com isquemia crônica crítica de membros: um estudo transversal
Authors: Santos Vanessa Prado dos , Cerutti Camila Izabel , Alencar Marcelo José Carlos , Queiroz André Brito , Ferreira Lucas de Mello , Fidelis Cícero , Araújo Filho José Siqueira de , Alves Carlos Alberto Silveira
Published: July 2023

Resumo Contexto Os fatores de risco para aterosclerose podem ter influência distinta nas doenças cardiovasculares, impactando também no território da doença arterial periférica (DAP). Objetivos Estudar a influência dos diferentes fatores de risco para a aterosclerose na distribuição anatômica da DAP em pacientes com isquemia crítica crônica de membros (ICCM). Métodos Foi realizado um estudo observacional, transversal e analítico, incluindo 476 doentes internados devido à ICCM por DAP. Foram estudados, comparativamente, os pacientes com DAP dos três distintos territórios anatômicos (aortoilíaco, femoropoplíteo e infragenicular) em relação à presença dos fatores de risco para a aterosclerose (idade, gênero, diabetes mellitus, tabagismo e hipertensão arterial). A análise multivariada foi realizada para testar a associação entre os fatores de risco e a distribuição anatômica da DAP. Resultados A média de idade dos pacientes foi de 69 anos, 249 (52%) eram homens, e 273 (57%), diabéticos. Predominaram os pacientes na Categoria 5 da Classificação de Rutherford (353/74%). Na análise multivariada, as mulheres tiveram chance 2,7 (IC: 1,75-4,26) vezes maior de doença do território femoropoplíteo. Os doentes tabagistas tiveram 3,6 (IC: 1,54-8,30) vezes maior risco de doença do território aortoilíaco. Diabéticos apresentaram 1,8 (IC: 1,04-3,19) vezes maior chance de obstrução apenas do território infragenicular. Conclusões O estudo mostrou que gênero, diabetes mellitus e tabagismo influenciam no padrão de distribuição da DAP em pacientes com ICCM. Os doentes diabéticos apresentaram maior chance de doença isolada do território infragenicular, as mulheres tiveram maior risco de DAP do território femoropoplíteo e os tabagistas possuem maior chance comprometimento aortoilíaco.
Síndrome de Dunbar - relato de caso
Authors: Campos Sarah Maria Lemos de , Pessoa Rafael Prado , Pelegrini João Paulo de Araújo , Silveira Henrique Fernandes , Diniz Maria Fernanda Lopes , Bianchini Maria Passos , Lopes Leonardo Soares , Costa Marcus Eduardo Valadares Meireles Martins da
Published: July 2023

Resumo A síndrome de Dunbar constitui um diagnóstico de exclusão dentro dos quadros de dor abdominal. O tratamento cirúrgico consiste na dissecção completa do ligamento e do gânglio nervoso circundante. Dessa forma, o presente caso refere-se a um paciente do sexo masculino, 45 anos, previamente hígido, com queixa de dor abdominal epigástrica com irradiação para o dorso e fraqueza. Inicialmente, foi realizada tomografia computadorizada de abdome para complementação do quadro, que evidenciou arteriopatia do tronco celíaco e da artéria mesentérica em associação com estenose. Optou-se por tratamento cirúrgico devido à refratariedade da dor, mas os achados foram inespecíficos. Houve necessidade de complementação da propedêutica com angiotomografia seriada para acompanhamento do caso. Após cerca de 6 meses, notou-se espessamento do ligamento arqueado, com compressão do terço proximal do tronco celíaco e estenose de 80%. Em meio a esse cenário, o paciente foi submetido a laparoscopia para descompressão do tronco celíaco, evoluindo satisfatoriamente no pós-operatório.