bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimizing and normalizing the population through hormone therapies in Italian science,c.1926–1950
Authors: BECCALOSSI CHIARA
Published: January 2020

This essay explores how hormone treatments were used to optimize and normalize individuals under Italian Fascism. It does so by taking the activities of the Biotypological Orthogenetic Institute − an Italian eugenics and endocrinological centre founded by Nicola Pende in 1926 − as the prime example of a version of eugenics, biotypology, which was based on hormone therapies. This essay first demonstrates that Italian Fascist biopolitics was not only concerned with increasing the size of the Italian population, but also with improving its quality. It suggests that under the Italian Fascist regime hormone therapies became eugenic tools of intervention to improve the Italian race. Second, while Pende's institute purportedly enhanced men and women, its activities show the extent to which the ‘techniques of normalization’ pursued by the Fascist regime were both systematic and invasive.
The influence of homeostatic mechanisms on neural regulation of food craving in anorexia nervosa
Authors: Stopyra Marion A. , Friederich Hans-Christoph , Mönning Esther , Lavandier Nora , Bendszus Martin , Herzog Wolfgang , Simon Joe J.
Published: January 2020

Background

Restrictive food intake in anorexia nervosa (AN) has been related to an overactive cognitive control network inhibiting intuitive motivational responses to food stimuli. However, the influence of short-term homeostatic signaling on the neural regulation of cue-induced food craving in AN is still unclear.

Methods

Twenty-five women with AN and 25 matched normal-weight women were examined on two occasions after receiving either glucose or water directly into their stomach using a nasogastric tube. Participants were blinded to the type of infusion. An event-related functional magnetic resonance imaging paradigm was used to investigate the effect of intestinal glucose load on neural processing during either simple viewing or distraction from food stimuli.

Results

Neural differences between patients with AN and normal-weight participants were found during the distraction from food stimuli, but not during the viewing condition. When compared to controls, patients with AN displayed increased activation during food distraction in the left parietal lobule/precuneus and fusiform gyrus after water infusion and decreased activation in ventromedial prefrontal and cingulate regions after intestinal glucose load.

Conclusions

Independent of the cephalic phase and the awareness of caloric intake, homeostatic influences trigger disorder-specific reactions in AN. Food distraction in patients with AN is associated with either excessive higher-order cognitive control during physiological hunger or decreased internally directed attention after intestinal glucose load. These findings suggest that food distraction plays an important role in the psychopathology of AN. This study was registered on clinicaltrials.gov with identifier: NCT03075371.
Navigation of Semi-autonomous Service Robots Using Local Information and Anytime Motion Planners
Authors: Pereira Guilherme A. S. , Freitas Elias J. R.
Published: January 2020

This paper deals with the problem of navigating semi-autonomous mobile robots without global localization systems in unknown environments. We propose a planning-based obstacle avoidance strategy that relies on local maps and a series of short-time coordinate frames. With this approach, simple odometry and range information are sufficient to make the robot to safely follow the user commands. Different from reactive obstacle avoidance strategies, the proposed approach chooses a good and smooth local path for the robot. The methodology is evaluated using a mobile service robot moving in an unknown corridor environment populated with obstacles and people.
A tuberculosis outbreak in a psychiatric hospital: Kanagawa, Japan, 2012
Authors: Tasaka M. , Koeda E. , Takahashi C. , Ota M.
Published: January 2020

In January 2012, an inpatient in a ward of a psychiatric hospital with nearly 300 beds in Kanagawa, Japan, was diagnosed with sputum smear-positive pulmonary tuberculosis (TB). Here we characterise the TB outbreak cases and identify the population at risk. TB was diagnosed when a person tested bacteriologically positive for TB or was determined to have TB by a physician. A latent TB infection (LTBI) case was defined as a person tested positive by interferon-gamma release assay (IGRA). A total of 125 contacts were screened via IGRA and chest X-ray. In all, 15 TB and 15 LTBI cases were found by the end of October 2012, and thereafter no additional TB case was found. Of the 15 TB cases, eight were culture-positive and all the isolates had identical variable number tandem repeat patterns. Twenty-four of the 56 (42.9%, 95% confidence interval (CI) 29.7–56.8) inpatients in the ward had either TB or LTBI with a relative risk of 8.6 (95% CI 1.2–59.3), compared to the staff members who did not work full-time in the ward (one of 20 (5.0%, 95% CI 0.0–24.9)). We recommend that psychiatric hospitals conduct periodic screening of staff members and inpatients for TB to prevent nosocomial TB outbreaks.
Drivers of population change in common farmland birds in Germany
Authors: BUSCH MALTE , KATZENBERGER JAKOB , TRAUTMANN SVEN , GERLACH BETTINA , DRÖSCHMEISTER RAINER , SUDFELDT CHRISTOPH
Published: January 2020

Farmland bird populations in Germany are declining at a higher speed than species inhabiting other habitats. We studied potential causes for bird population changes based on data from standardised German breeding bird monitoring schemes. We related population trends to covariates describing the changes in the agricultural landscape in Germany, weather conditions during the breeding season and for some migratory species, conditions at stopover and wintering sites. Linear mixed effect models were used to analyse effect strength at species level and conclusions are drawn for the overall group of farmland bird species. The area of grassland and fallow land was shown to have the strongest positive effects and the area of maize and rapeseed the strongest negative effects on farmland bird population trends. The results obtained also indicate that despite the consistent influence of weather conditions during the breeding season, land-use changes had a stronger impact on bird populations than weather. Conditions at Sahel wintering sites did not show a consistent effect on population trends. Based on these findings the study quantitatively underpins and ranks key factors shaping farmland bird populations in Germany.
Effects of different polyvinylpyrrolidone concentrations on intracytoplasmic sperm injection
Authors: Ding Ding , Wang Qiushuang , Li Xinyuan , Chen Beili , Zou Weiwei , Ji Dongmei , Hao Yan , Xue Rufeng , Zou Huijuan , Wei Zhaolian , Zhou Ping , Cao Yunxia , Zhang Zhiguo
Published: January 2020

To explore whether different polyvinylpyrrolidone (PVP) concentrations affect the results of intracytoplasmic sperm injection (ICSI), a prospective study was conducted for 194 couples undergoing 210 ICSI therapy cycles. These cycles were divided into three groups (10, 7 and 5% groups) using the corresponding concentration of PVP for sperm immobilization. The main outcome measures were analyzed. Results indicated that, with a decrease in PVP concentrations, all of the main outcome measures increased. In particular, the high-quality cleavage embryo rate in the 7% group was significantly lower than in the 5% group (P < 0.01), and the cleavage, high-quality cleavage embryo, and high-quality blastocyst rates in the 5% group were significantly higher than those in the 10% group (all P < 0.001). For high-/intermediate-quality semen, all of the main outcome measures were significantly increased with 5% PVP. For the poor-quality semen, only the high-quality cleavage embryo and high-quality blastocyst rates were significantly higher in the 5% group. Therefore, lowering PVP concentrations greatly promoted the development of embryos in ICSI cycles, with an optimal concentration of 5% for ICSI.
Micromorphological and ultrastructural description of spermatozoa from squirrel monkeys (Saimiri collinsi Osgood, 1916)
Authors: Leão Danuza L. , Sampaio Wlaisa V. , Sousa Patrícia C. , Moura Arlindo A.A. , Oskam Irma C. , Santos Regiane R. , Domingues Sheyla F.S.
Published: January 2020

Saimiri collinsi is used as an animal model in biotechnology research for conservation of species from the genus Saimiri. However, the development of biotechnologies depends on a proper knowledge of the sperm morphology to understand the basic aspects of sperm physiology, as potential male fertility depends on different cellular sperm structures. With this purpose, this study characterized the micromorphological and ultrastructural characteristics of squirrel monkeys (Saimiri collinsi) sperm using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM electromyography revealed that a normal Saimiri collinsi sperm measures 71.7 ± 0.7 μm with lateral tail insertion, a paddle-shaped flattened head and an acrosome occupying most of the head. TEM also showed that the middle piece is characterized by a central 9 + 2 microtubule axoneme surrounded by nine dense fibres, and that the mitochondria were juxtaposed, forming the mitochondrial sheath. Here we provide the first micromorphological and ultrastructure description of S. collinsi sperm.
Evaluation of a multimodal pain rehabilitation programme in primary care based on clinical register data: a feasibility study
Authors: Sennehed Charlotte Post , Stigmar Kjerstin , Grahn Birgitta , Fischer Marcelo Rivano , Forsbrand Malin , Nyberg Anja , Petersson Ingemar F. , Holmberg Sara
Published: January 2020

Aim:

Investigate the feasibility of identifying a well-defined treatment group and a comparable reference group in clinical register data.

Background:

There is insufficient knowledge on how to avert neck/back pain from turning chronic or to impair work ability. The Swedish Government implemented a national multimodal rehabilitation (MMR) programme in primary care intending to promote work ability, reduce sick leave and increase return to work. Since randomised control trial data for effect is lacking, it is important to evaluate existing observational data from clinical settings.

Methods:

We identified all unique patients with musculoskeletal pain (MSP) diagnoses undergoing the MMR programme in primary care in the Skåne Health care Register (n = 2140) during 2010–2011. A reference cohort in primary care (n = 56 300) with similar MSP diagnoses, same ages and the same level of sick leave before baseline was identified for the same period. The reference cohort received ordinary care and treatment in primary care. The final study group consisted of 603 eligible MMR patients and 2874 eligible reference patients. Socio-economic and health-related baseline data including sick leave one year before up to two years after baseline were compared between groups.

Findings:

There were significant socio-economic and health differences at baseline between the MMR and the reference patients, with the MMR group having lower income, higher morbidity and more sick leave days. Sick leave days per year decreased significantly in the MMR group (118–102 days, P < 0.001) and in the reference group (50–42 days, P < 0.001) from one year before baseline to two years after.

Conclusions:

It was not feasible to identify a comparable reference group based on clinical register data. Despite an ambitious attempt to limit selection bias, significant baseline differences in socio-economic and health were present. In absence of randomised trials, effects of MMR cannot be sufficiently evaluated in primary care.
What's it like to be a state? An argument for state consciousness
Authors: Lerner Adam B.
Published: January 2020

Questions of consciousness pervade the social sciences. Yet, despite persistent tendencies to anthropomorphize states, most International Relations scholarship implicitly adopts the position that humans are conscious and states are not. Recognizing that scholarly disagreement over fundamental issues prevents answering definitively whether states are truly conscious, I instead demonstrate how scholars of multiple dispositions can incorporate a pragmatic notion of state consciousness into their theorizing. Drawing on recent work from Eric Schwitzgebel and original supplementary arguments, I demonstrate that states are not only complex informationally integrated systems with emergent properties, but they also exhibit seemingly genuine responses to qualia that are irreducible to individuals within them. Though knowing whether states possess an emergent ‘stream’ of consciousness indiscernible to their inhabitants may not yet be possible, I argue that a pragmatic notion of state consciousness can contribute to a more complete understanding of state personhood, as well as a revised model of the international system useful to multiple important theoretical debates. In the article's final section, I apply this model to debate over the levels of analysis at which scholarship applies ontological security theory. I suggest the possibility of emergent state-level ontological insecurity that need not be understood via problematic reduction to individuals.
Design of ultra-wide tetra band phased array inverted T-shaped patch antennas using DGS with beam-steering capabilities for 5G applications
Authors: Anas Muhammad , Shahid Hifsa , Rauf Abdul , Shahid Abdullah
Published: January 2020

A novel 1 × 4 phased array elliptical inverted T-shaped slotted sectored patch antenna with defected ground structure (DGS), resonate at proposed ultra-wide tetra band at 28, 43, 51, and 64 GHz with high gain and beam-steering capabilities is presented. An inverted T-shaped slotted stub is used with the sectored patch to achieve ultra-wideband properties. In order to resonate the antenna at four different bands, DGS of round bracket slot is etched on the ground. The 1 × 4 phased arrays are used at the top edge and bottom edge of mobile PCB with high gain. The simulation results show that the antenna has four ultra-wide bands: 25.8–29.7, 40.6–44.6, 49.2–53.1, and 62.2–74 GHz with a maximum gain of 16.5 dBi at 51 GHz. The phased array antenna is capable to steer its main beam within ±30° at the 26, 28, and 43 GHz, using appropriate phase shifts of each antenna element. The proposed millimeter wave antenna is particularly suitable for cellular infrastructures and can be a candidate for emerging 5G mobile applications. The availability of an additional 11.8 GHz (62.2–74 GHz) of contiguous unlicensed spectrum will allow the launching of new exciting wireless services.
TwinsMX: Uncovering the Basis of Health and Disease in the Mexican Population
Authors: Leon-Apodaca Ana V. , Chiu-Han Enrique , Ortega-Mora Ivett , Román-López Talía V. , Caballero-Sánchez Ulises , Aldana-Assad Oscar , Campos Adrián I. , Cuellar-Partida Gabriel , Ruiz-Contreras Alejandra E. , Alcauter Sarael , Rentería Miguel E. , Medina-Rivera Alejandra
Published: January 2020

TwinsMX is a national twin registry in Mexico recently created with institutional support from the Universidad Nacional Autónoma de México. It aims to serve as a platform to advance epidemiological and genetic research in the country and to disentangle the genetic and environmental contributions to health and disease in the admixed Mexican population. Here, we describe our recruitment and data collection strategies and discuss both the progress to date and future directions. More information about the registry is available on our website: https://twinsmxofficial.unam.mx/ (content in Spanish).
Nonlinear Impacts of Surface Exchange Coefficient Uncertainty on Tropical Cyclone Intensity and Air‐Sea Interactions
Authors: Nystrom Robert G. , Chen Xingchao , Zhang Fuqing , Davis Christopher A.
Published: January 2020

Tropical cyclone maximum intensity is believed to result from a balance between the surface friction, which removes energy, and a temperature/moisture (enthalpy) difference between the sea surface and the air above it, which adds energy. The competing processes near the air‐sea interface are controlled by both the near surface wind speed and the surface momentum (Cd) and enthalpy (Ck) exchange coefficients. Unfortunately, these coefficients are currently highly uncertain at high wind speeds. Tropical cyclone winds also apply a force on the ocean surface, which results in ocean surface cooling through vertical mixing. Using coupled atmosphere‐ocean and uncoupled (atmosphere only) ensemble simulations we explore the complex influence of uncertain surface exchange coefficients on storm‐induced ocean feedback and tropical cyclone intensity. We find that the magnitude of ocean cooling increases with storm intensity and Cd. Additionally, the simulated maximum wind speed uncertainty does not necessarily decrease when ocean feedback are considered.
Methane Mitigation: Methods to Reduce Emissions, on the Path to the Paris Agreement
Authors: Nisbet E. G. , Fisher R. E. , Lowry D. , France J. L. , Allen G. , Bakkaloglu S. , Broderick T. J. , Cain M. , Coleman M. , Fernandez J. , Forster G. , Griffiths P. T. , Iverach C. P. , Kelly B. F. J. , Manning M. R. , Nisbet‐Jones P. B. R. , Pyle J. A. , Townsend‐Small A. , al‐Shalaan A. , Warwick N. , Zazzeri G.
Published: January 2020

The atmospheric methane burden is increasing rapidly, contrary to pathways compatible with the goals of the 2015 United Nations Framework Convention on Climate Change Paris Agreement. Urgent action is required to bring methane back to a pathway more in line with the Paris goals. Emission reduction from “tractable” (easier to mitigate) anthropogenic sources such as the fossil fuel industries and landfills is being much facilitated by technical advances in the past decade, which have radically improved our ability to locate, identify, quantify, and reduce emissions. Measures to reduce emissions from “intractable” (harder to mitigate) anthropogenic sources such as agriculture and biomass burning have received less attention and are also becoming more feasible, including removal from elevated‐methane ambient air near to sources. The wider effort to use microbiological and dietary intervention to reduce emissions from cattle (and humans) is not addressed in detail in this essentially geophysical review. Though they cannot replace the need to reach “net‐zero” emissions of CO2, significant reductions in the methane burden will ease the timescales needed to reach required CO2reduction targets for any particular future temperature limit. There is no single magic bullet, but implementation of a wide array of mitigation and emission reduction strategies could substantially cut the global methane burden, at a cost that is relatively low compared to the parallel and necessary measures to reduce CO2, and thereby reduce the atmospheric methane burden back toward pathways consistent with the goals of the Paris Agreement.
Correction: Autoantibodies to synapsin I sequestrate synapsin I and alter synaptic function
Authors: Rocchi Anna , Sacchetti Silvio , De Fusco Antonio , Giovedi Silvia , Parisi Barbara , Cesca Fabrizia , Höltje Markus , Ruprecht Klemens , Ahnert-Hilger Gudrun , Benfenati Fabio
Published: January 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Biphasic exocytosis of herpesvirus from hippocampal neurons and mechanistic implication to membrane fusion
Authors: Liu Yun-Tao , Shivakoti Sakar , Jia Fan , Tao Chang-Lu , Zhang Bin , Xu Fuqiang , Lau Pakming , Bi Guo-Qiang , Zhou Z. Hong
Published: January 2020

Exocytosis is a crucial cellular process involved in the release of neural transmitters or signaling hormones, and disposal of waste or toxic materials. The relationship between structural transition and temporal progression of this process is poorly understood, partly due to lack of adequate tools to resolve such dynamic structures at sufficient resolution in 3D. Exocytosis can be hijacked by some viruses, exemplified by the widely used model α-herpesvirus pseudorabies virus (PRV), which relies on exocytosis for trans-synaptic spread across neurons. Here, we have used cryo electron tomography (cryoET) to capture 199 events of PRV exocytosis from cultured hippocampal neurons. We established cumulative frequency analysis to estimate the relative duration of an exocytosis stage based on the frequency of observed viral particles at that stage. This analysis revealed that PRV exocytosis is biphasic, including a fast, “release phase” driven by fusion proteins and fused membranes, and a slow, “recovery phase” driven by flattening of curved membranes. The biphasic property of exocytosis discovered here appears to be conserved for membrane fusion during viral entry, and our approach of cumulative frequency analysis should have general utility for characterizing other membrane fusion events.
The ETFL formulation allows multi-omics integration in thermodynamics-compliant metabolism and expression models
Authors: Salvy Pierre , Hatzimanikatis Vassily
Published: January 2020

Systems biology has long been interested in models capturing both metabolism and expression in a cell. We propose here an implementation of the metabolism and expression model formalism (ME-models), which we call ETFL, for Expression and Thermodynamics Flux models. ETFL is a hierarchical model formulation, from metabolism to RNA synthesis, that allows simulating thermodynamics-compliant intracellular fluxes as well as enzyme and mRNA concentration levels. ETFL formulates a mixed-integer linear problem (MILP) that enables both relative and absolute metabolite, protein, and mRNA concentration integration. ETFL is compatible with standard MILP solvers and does not require a non-linear solver, unlike the previous state of the art. It also accounts for growth-dependent parameters, such as relative protein or mRNA content. We present ETFL along with its validation using results obtained from a well-characterized E. coli model. We show that ETFL is able to reproduce proteome-limited growth. We also subject it to several analyses, including the prediction of feasible mRNA and enzyme concentrations and gene essentiality.
Dorsal root ganglion macrophages contribute to both the initiation and persistence of neuropathic pain
Authors: Yu Xiaobing , Liu Hongju , Hamel Katherine A. , Morvan Maelig G. , Yu Stephen , Leff Jacqueline , Guan Zhonghui , Braz Joao M. , Basbaum Allan I.
Published: January 2020

Paralleling the activation of dorsal horn microglia after peripheral nerve injury is a significant expansion and proliferation of macrophages around injured sensory neurons in dorsal root ganglia (DRG). Here we demonstrate a critical contribution of DRG macrophages, but not those at the nerve injury site, to both the initiation and maintenance of the mechanical hypersensitivity that characterizes the neuropathic pain phenotype. In contrast to the reported sexual dimorphism in the microglial contribution to neuropathic pain, depletion of DRG macrophages reduces nerve injury-induced mechanical hypersensitivity and expansion of DRG macrophages in both male and female mice. However, fewer macrophages are induced in the female mice and deletion of colony-stimulating factor 1 from sensory neurons, which prevents nerve injury-induced microglial activation and proliferation, only reduces macrophage expansion in male mice. Finally, we demonstrate molecular cross-talk between axotomized sensory neurons and macrophages, revealing potential peripheral DRG targets for neuropathic pain management.
Nanoscopic diffusion of water on a topological insulator
Authors: Tamtögl Anton , Sacchi Marco , Avidor Nadav , Calvo-Almazán Irene , Townsend Peter S. M. , Bremholm Martin , Hofmann Philip , Ellis John , Allison William
Published: January 2020

The microscopic motion of water is a central question, but gaining experimental information about the interfacial dynamics of water in fields such as catalysis, biophysics and nanotribology is challenging due to its ultrafast motion, and the complex interplay of inter-molecular and molecule-surface interactions. Here we present an experimental and computational study of the nanoscale-nanosecond motion of water at the surface of a topological insulator (TI), Bi$${}_{2}$$2Te$${}_{3}$$3. Understanding the chemistry and motion of molecules on TI surfaces, while considered a key to design and manufacturing for future applications, has hitherto been hardly addressed experimentally. By combining helium spin-echo spectroscopy and density functional theory calculations, we are able to obtain a general insight into the diffusion of water on Bi$${}_{2}$$2Te$${}_{3}$$3. Instead of Brownian motion, we find an activated jump diffusion mechanism. Signatures of correlated motion suggest unusual repulsive interactions between the water molecules. From the lineshape broadening we determine the diffusion coefficient, the diffusion energy and the pre-exponential factor.
Dynamic organelle distribution initiates actin-based spindle migration in mouse oocytes
Authors: Duan Xing , Li Yizeng , Yi Kexi , Guo Fengli , Wang HaiYang , Wu Pei-Hsun , Yang Jing , Mair Devin B. , Morales Edwin Angelo , Kalab Petr , Wirtz Denis , Sun Sean X. , Li Rong
Published: January 2020

Migration of meiosis-I (MI) spindle from the cell center to a sub-cortical location is a critical step for mouse oocytes to undergo asymmetric meiotic cell division. In this study, we investigate the mechanism by which formin-2 (FMN2) orchestrates the initial movement of MI spindle. By defining protein domains responsible for targeting FMN2, we show that spindle-periphery localized FMN2 is required for spindle migration. The spindle-peripheral FMN2 nucleates short actin bundles from vesicles derived likely from the endoplasmic reticulum (ER) and concentrated in a layer outside the spindle. This layer is in turn surrounded by mitochondria. A model based on polymerizing actin filaments pushing against mitochondria, thus generating a counter force on the spindle, demonstrated an inherent ability of this system to break symmetry and evolve directional spindle motion. The model is further supported through experiments involving spatially biasing actin nucleation via optogenetics and disruption of mitochondrial distribution and dynamics.
Environmental DNA reveals seasonal shifts and potential interactions in a marine community
Authors: Djurhuus Anni , Closek Collin J. , Kelly Ryan P. , Pitz Kathleen J. , Michisaki Reiko P. , Starks Hilary A. , Walz Kristine R. , Andruszkiewicz Elizabeth A. , Olesin Emily , Hubbard Katherine , Montes Enrique , Otis Daniel , Muller-Karger Frank E. , Chavez Francisco P. , Boehm Alexandria B. , Breitbart Mya
Published: January 2020

Environmental DNA (eDNA) analysis allows the simultaneous examination of organisms across multiple trophic levels and domains of life, providing critical information about the complex biotic interactions related to ecosystem change. Here we used multilocus amplicon sequencing of eDNA to survey biodiversity from an eighteen-month (2015–2016) time-series of seawater samples from Monterey Bay, California. The resulting dataset encompasses 663 taxonomic groups (at Family or higher taxonomic rank) ranging from microorganisms to mammals. We inferred changes in the composition of communities, revealing putative interactions among taxa and identifying correlations between these communities and environmental properties over time. Community network analysis provided evidence of expected predator-prey relationships, trophic linkages, and seasonal shifts across all domains of life. We conclude that eDNA-based analyses can provide detailed information about marine ecosystem dynamics and identify sensitive biological indicators that can suggest ecosystem changes and inform conservation strategies.
Digitally virtualized atoms for acoustic metamaterials
Authors: Cho Choonlae , Wen Xinhua , Park Namkyoo , Li Jensen
Published: January 2020

By designing tailor-made resonance modes with structured atoms, metamaterials allow us to obtain constitutive parameters outside their limited range from natural materials. Nonetheless, tuning the constitutive parameters depends on our ability to modify the physical structure or external circuits attached to the metamaterials, posing a fundamental challenge to the range of tunability in many real-time applications. Here, we propose the concept of virtualized metamaterials on their signal response function to escape the boundary inherent in the physical structure of metamaterials. By replacing the resonating physical structure with a designer mathematical convolution kernel with a fast digital signal processing circuit, we demonstrate a decoupled control of the effective bulk modulus and mass density of acoustic metamaterials on-demand through a software-defined frequency dispersion. Providing freely software-reconfigurable amplitude, center frequency, bandwidth of frequency dispersion, our approach adds an additional dimension to constructing non-reciprocal, non-Hermitian, and topological systems with time-varying capability as potential applications.
Market integration reduces kin density in women’s ego-networks in rural Poland
Authors: Colleran Heidi
Published: January 2020

It is widely assumed that as populations become more market integrated the ‘inner circles’ of people’s social networks become less densely connected and family-oriented. This ‘loosening’ of kin networks may fundamentally alter the social dynamics of reproduction, facilitating demographic transitions to low fertility. Few data exist to test this hypothesis. Previous research in urbanized populations has not explicitly measured kin density in ego-networks, nor assessed how market integration influences network structure at different levels of aggregation. Here I analyze the ego-networks of ~2000 women in 22 rural Polish communities transitioning from subsistence farming to market-dependence. I compare how ego-network size, density and kin density co-vary with household and community-level market integration. Market integration is associated with less kin-dense networks, but not necessarily less dense ones, and is unrelated to network size. Declining kin density during economic transitions may be a critical mechanism for the broader cultural transmission of low fertility values.
Extreme genetic signatures of local adaptation during Lotus japonicus colonization of Japan
Authors: Shah Niraj , Wakabayashi Tomomi , Kawamura Yasuko , Skovbjerg Cathrine Kiel , Wang Ming-Zhuo , Mustamin Yusdar , Isomura Yoshiko , Gupta Vikas , Jin Haojie , Mun Terry , Sandal Niels , Azuma Fuyuki , Fukai Eigo , Seren Ümit , Kusakabe Shohei , Kikuchi Yuki , Nitanda Shogo , Kumaki Takashi , Hashiguchi Masatsugu , Tanaka Hidenori , Hayashi Atsushi , Sønderkær Mads , Nielsen Kaare Lehmann , Schneeberger Korbinian , Vilhjalmsson Bjarni , Akashi Ryo , Stougaard Jens , Sato Shusei , Schierup Mikkel Heide , Andersen Stig Uggerhøj
Published: January 2020

Colonization of new habitats is expected to require genetic adaptations to overcome environmental challenges. Here, we use full genome re-sequencing and extensive common garden experiments to investigate demographic and selective processes associated with colonization of Japan by Lotus japonicus over the past ~20,000 years. Based on patterns of genomic variation, we infer the details of the colonization process where L. japonicus gradually spread from subtropical conditions to much colder climates in northern Japan. We identify genomic regions with extreme genetic differentiation between northern and southern subpopulations and perform population structure-corrected association mapping of phenotypic traits measured in a common garden. Comparing the results of these analyses, we find that signatures of extreme subpopulation differentiation overlap strongly with phenotype association signals for overwintering and flowering time traits. Our results provide evidence that these traits were direct targets of selection during colonization and point to associated candidate genes.
Author Correction: Quantum nanophotonics with group IV defects in diamond
Authors: Bradac Carlo , Gao Weibo , Forneris Jacopo , Trusheim Matthew E. , Aharonovich Igor
Published: January 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Waves on Louisiana Continental Shelf Influenced by Atmospheric Fronts
Authors: Guo Biyun , Subrahmanyam M. V. , Li C.
Published: January 2020

Ocean-atmospheric dynamical processes influence the wave characteristics, and sea surface temperature (SST). The processes that affect SST in the ocean area included surface heat fluxes, wind, and precipitation. In this study, we analyzed the wave data in response to the cold front passages over Louisiana continental shelf. The data examined in this research is mainly from WAVCIS (Wave-Current-surge Information System), Coastal Ocean Estuarine Dynamics Lab at Louisiana State University and Global Precipitation Climatology Project (GPCP). With respect to the wave response to the atmospheric forcing, here we consider: (1) the connection between wave variability and atmospheric frontal passages, and (2) the influence on the SST variations during the processes. The occurrences of wind wave, precipitation, and weather processes have distinct regularities. Atmospheric cold fronts have an important influence on wave formation and associated processes over Louisiana continental shelf.
Investigating a Plasmodium falciparum erythrocyte invasion phenotype switch at the whole transcriptome level
Authors: Nyarko Prince B. , Tarr Sarah J. , Aniweh Yaw , Stewart Lindsay B. , Conway David J. , Awandare Gordon A.
Published: January 2020

The central role that erythrocyte invasion plays in Plasmodium falciparum survival and reproduction makes this process an attractive target for therapeutic or vaccine development. However, multiple invasion-related genes with complementary and overlapping functions afford the parasite the plasticity to vary ligands used for invasion, leading to phenotypic variation and immune evasion. Overcoming the challenge posed by redundant ligands requires a deeper understanding of conditions that select for variant phenotypes and the molecular mediators. While host factors including receptor heterogeneity and acquired immune responses may drive parasite phenotypic variation, we have previously shown that host-independent changes in invasion phenotype can be achieved by continuous culturing of the W2mef and Dd2 P. falciparum strains in moving suspension as opposed to static conditions. Here, we have used a highly biologically replicated whole transcriptome sequencing approach to identify the molecular signatures of variation associated with the phenotype switch. The data show increased expression of particular invasion-related genes in switched parasites, as well as a large number of genes encoding proteins that are either exported or form part of the export machinery. The genes with most markedly increased expression included members of the erythrocyte binding antigens (EBA), reticulocyte binding homologues (RH), surface associated interspersed proteins (SURFIN), exported protein family 1 (EPF1) and Plasmodium Helical Interspersed Sub-Telomeric (PHIST) gene families. The data indicate changes in expression of a repertoire of genes not previously associated with erythrocyte invasion phenotypes, suggesting the possibility that moving suspension culture may also select for other traits.
Complex marine bioturbation ecosystem engineering behaviors persisted in the wake of the end-Permian mass extinction
Authors: Cribb Alison T. , Bottjer David J.
Published: January 2020

The end-Permian mass extinction was the most severe mass extinction event of the Phanerozoic and was followed by a several million-year delay in benthic ecosystem recovery. While much work has been done to understand biotic recovery in both the body and trace fossil records of the Early Triassic, almost no focus has previously been given to analyzing patterns in ecosystem engineering complexity as a result of the extinction drivers. Bioturbation is a key ecosystem engineering behavior in marine environments, as it results in changes to resource flows and the physical environment. Thus, the trace fossil record can be used to examine the effect of the end-Permian mass extinction on bioturbating ecosystem engineers. We present a dataset compiled from previously published literature to analyze burrowing ecosystem engineering behaviors through the Permian-Triassic boundary. We report two key observations: first, that there is no loss in bioturbation ecosystem engineering behaviors after the mass extinction, and second, that these persisting behaviors include deep tier, high-impact, complex ecosystem engineering. These findings suggest that while environmental conditions may have limited deeper burrowing, complex ecosystem engineering behaviors were able to persist in the Early Triassic. Furthermore, the persistence of deep tier bioirrigated three-dimensional network burrows implies that benthic biogeochemical cycling could have been maintained at pre-extinction states in some local environments, stimulating ecosystem productivity and promoting biotic recovery in the Early Triassic.
Thermal sensitivity links to cellular cardiac decline in three spiny lobsters
Authors: Oellermann Michael , Hickey Anthony J. R. , Fitzgibbon Quinn P. , Smith Greg
Published: January 2020

Understanding mechanisms of thermal sensitivity is key to predict responses of marine organisms to changing temperatures. Sustaining heart function is critical for complex organisms to oxygenate tissues, particularly under temperature stress. Yet, specific mechanisms that define thermal sensitivity of cardiac function remain unclear. Here we investigated whole animal metabolism, cardiac performance and mitochondrial function in response to elevated temperatures for temperate, subtropical and tropical spiny lobster species. While oxygen demands increased with rising temperatures, heart function became limited or declined in all three species of lobsters. The decline in cardiac performance coincided with decreases in mitochondrial efficiency through increasing mitochondrial proton leakage, which predicts impaired compensation of ATP production. Species differences were marked by shifts in mitochondrial function, with the least thermal scope apparent for tropical lobsters. We conclude that acute temperature stress of spiny lobsters, irrespective of their climatic origin, is marked by declining cellular energetic function of the heart, contributing to an increasing loss of whole animal performance. Better understanding of physiological thermal stress cascades will help to improve forecasts of how changing environmental temperatures affect the fitness of these ecologically and commercially important species.
Vitamin D Supplementation in Overweight/obese Asian Indian Women with Prediabetes Reduces Glycemic Measures and Truncal Subcutaneous Fat: A 78 Weeks Randomized Placebo-Controlled Trial (PREVENT-WIN Trial)
Authors: Bhatt Surya Prakash , Misra Anoop , Pandey Ravindra Mohan , Upadhyay Ashish Datt , Gulati Seema , Singh Namrata
Published: January 2020

Vitamin D deficiency may contribute to etiology of type 2 diabetes in Asian Indians. The objectives of this study was to evaluate effect of vitamin D supplementation on glycemic profile and body composition in prediabetic and vitamin D deficient overweight/obese Asian Indian women. In this open-label randomized placebo-controlled trial (78 weeks duration), 121 females (aged 20–60 years) with prediabetes and vitamin D deficiency were randomly allocated in intervention (n, 61) and placebo (n, 60) groups. The primary outcome variables were fasting blood glucose (FBG), 2-h blood glucose post OGTT (2-h BG), hemoglobin A1c (HbA1C), and reversal to normoglycemia. In Intention-to-treat analysis, at the end of intervention, we observed significant decrease in FBG [−5.0 (−12.6–2.4), p = 0.04], 2-h blood glucose post OGTT [−11(−49.3–26.9), p = 0.02], hemoglobin A1c [−0.41 (5.89, 6.55), p = 0.05] and increase in 25(OH) D [7.5 (−6.0–20.9), p = 0.002] levels in intervention as compared to the placebo group. Changes in glycemic category based on FBG were as follows; intervention group: normal FBG, 58.6%; impaired fasting glucose (IFG), 39%; and type 2 diabetes mellitus (T2DM), 2.4%; placebo group: normal FBG, 48.8%; IFG, 46.3%; and T2DM, 4.9%. Changes in category of 2-hour glucose post OGTT after intervention were as follows; intervention group: normal glucose tolerance (NGT) 51.2% and prediabetes, 48.8%; placebo group: NGT, 43.9%; prediabetes, 53.7% and T2DM, 2.4%. After intervention, subscapular skinfold (visit Ist compared to visit IIIrd) and suprailiac skinfold (visit IInd compared to visit IIIrd) were significantly lower in intervention group vs. control group. In conclusion, we observed significant reduction in FBG, 2-hour glucose post OGTT, HbA1c, and truncal subcutaneous fat and reversal to normoglycemia in overweight/obese prediabetic vitamin D deficient Asian Indian women after 78 weeks of vitamin D supplementation.
Population-specific signatures of intra-individual mitochondrial DNA heteroplasmy and their potential evolutionary advantages
Authors: Tikochinski Yaron , Carreras Carlos , Tikochinski Gili , Vilaça Sibelle T.
Published: January 2020

Heteroplasmy is the existence of more than one mitochondrial DNA (mtDNA) variant within a cell. The evolutionary mechanisms of heteroplasmy are not fully understood, despite being a very common phenomenon. Here we combined heteroplasmy measurements using high throughput sequencing on green turtles (Chelonia mydas) with simulations to understand how heteroplasmy modulates population diversity across generations and under different demographic scenarios. We found heteroplasmy to be widespread in all individuals analysed, with consistent signal in individuals across time and tissue. Significant shifts in haplotype composition were found from mother to offspring, signalling the effect of the cellular bottleneck during oogenesis as included in the model. Our model of mtDNA inheritance indicated that heteroplasmy favoured the increase of population diversity through time and buffered against population bottlenecks, thus indicating the importance of this phenomenon in species with reduced population sizes and frequent population bottlenecks like marine turtles. Individuals with recent haplotypes showed higher levels of heteroplasmy than the individuals with ancient haplotypes, suggesting a potential advantage of maintaining established copies when new mutations arise. We recommend using heteroplasmy through high throughput sequencing in marine turtles, as well as other wildlife populations, for diversity assessment, population genetics, and mixed stock analysis.
Superdirective dimers of coupled self-resonant split ring resonators: Analytical modelling and numerical and experimental validation
Authors: Vallecchi A. , Radkovskaya A. , Li L. , Faulkner G. , Stevens C. J. , Shamonina E.
Published: January 2020

Superdirective antennas developed over the last century have received renewed interest in recent years from the development of metamaterials. These arrays of electromagnetic resonators (or meta-atoms) carrying short wavelength electro- and/or magneto-inductive waves support current distributions with very high spatial frequency as required by the classical conditions for superdirectivity. As meta-atoms can have both electric and magnetic dipole characteristics (and hence radiation properties), developing antennas exploiting these distributions can challenge conventional intuitions regarding the optimal configurations required. In this work we are reporting the development of a genuinely superdirective array using split ring resonators (SRRs). We provide a comprehensive analytical model characterizing the radiation from SRR dimers in which excitation of only one split ring leads to superdirective radiation via mutually coupled modes. Our model exploits simple circuit descriptions of coupled resonant circuits, combined with standard radiation formulae for curvilinear current distributions. Using this simple model we are able to map directivity against possible SRR locations and orientations in two dimensions and identify the unique optimal configuration which meets the requirements for superdirective emission. We validate the theoretical findings by comparison to both full wave simulations and experiments showing that our SRR dimer achieves endfire directivity very close to the maximum theoretical value.
Impact of BH3-mimetics on Human and Mouse Blood Leukocytes: A Comparative Study
Authors: Rohner Lionel , Reinhart Ramona , Iype Joseena , Bachmann Sofia , Kaufmann Thomas , Fux Michaela
Published: January 2020

BH3-mimetics are small molecule inhibitors that neutralize the function of anti-apoptotic BCL-2 family members. BH3-mimetics have recently gained a lot of popularity in oncology because of their success in cancer treatment. However, BH3-mimetics might have a broader clinical application. Here, we established an ex vivo flow cytometric assay allowing the comparison of the impact of BH3-mimetics (ABT-199, ABT-263, WEHI-539, and S63845) on leukocyte populations of both, healthy human subjects and C57BL/6 J wild type mice. BH3-mimetics were added to freshly drawn blood that was diluted 1/2 in cell medium, and BH3-mimetics-mediated impact on leukocyte count was assessed by flow cytometry. Our results demonstrate that responses towards 1μM of BH3-mimetics can be identical as well as considerably different in leukocytes of humans and mice. For instance, the inhibition of BCL-2 by ABT-199 caused cell death in all types of lymphocytes in mice but was exclusively specific for B cells in humans. Moreover, inhibition of BCL-XL by WEHI-539 affected solely mouse leukocytes while targeting MCL-1 by S63845 resulted in efficient induction of cell death in human neutrophils but not in their mouse counterparts. Our ex vivo assay enables initial identification of analogies and differences between human and mouse leukocytes in response towards BH3-mimetics.
Unraveling the Role of Interfaces on the Spall Failure of Cu/Ta Multilayered Systems
Authors: Chen Jie , Mathaudhu Suveen N. , Thadhani Naresh , Dongare Avinash M.
Published: January 2020

Molecular dynamics (MD) simulations are carried out to investigate the effects of the type and spacing of FCC/BCC interfaces on the deformation and spall behavior. The simulations are carried out using model Cu/Ta multilayers with six different types of interfaces. The results suggest that interface type can significantly affect the structure and intensity of the incoming shock wave, change the activated slip systems, alter dislocation slip and twinning behavior, affect where and how voids are nucleated during spallation and the resulting spall strength. Moreover, the above aspects are significantly affected by the interface spacing. A transition from homogeneous to heterogeneous dislocation nucleation occurs as the interface spacing is decreased to 6 nm. Depending on interface type and spacing, damage (voids) nucleation and spall failure is observed to occur not only at the Cu/Ta interfaces, but also in the weaker Cu layer interior, or even in the stronger Ta layer interior, although different mechanisms underlie each of these three distinct failure modes. These findings point to the fact that, depending on the combination of interface type and spacing, interfaces can lead to both strengthening and weakening of the Cu/Ta multilayered microstructures.