166 Mitochondrial stress responses in bovine cumulus cells and oocytes matured under lipotoxic conditions: a proteomic insight
Authors: Marei W. F. A. , Van Raemdonck G. , Baggerman G. , Bols P. E. J. , Leroy J. L. M. R. 
Published: December 2018

Obesity and up-regulated lipolysis are commonly associated with increased free fatty acid (FFA) concentrations, predominantly palmitic acid (PA), in blood and ovarian follicular fluid, which have been strongly linked with reduced oocyte quality. Mitochondria are known to play a central role in regulating cellular stress responses to lipotoxicity, which is well described in somatic cells. Although mitochondrial functions in oocytes are crucial for developmental competence, their stress response capacity has not been clearly described. Networking with endoplasmic reticulum unfolded protein responses (UPR) and protein translation may be different in oocytes than in somatic cells. Understanding these mechanisms is important to develop treatments. The aim of this study was to compare PA-induced stress responses in oocytes to those in cumulus cells (CC). We exposed bovine cumulus-oocyte complexes (COC) to pathophysiological PA concentration (150 µM) or solvent during in vitro maturation (24h) as a model. Then, the CC were separated from the oocytes (from pools of 120 COC per treatment per replicate, 3 replicates), and their proteomic profiles were examined using shotgun proteomic analysis with tandem mass tags. Functional analysis of the differentially regulated proteins (DRP; P&lt;0.05, fold change &gt;10%, false discovery rate &lt;5%) was done using Ingenuity® Pathway Analysis (IPA®; Qiagen, Valencia, CA, USA). A total of 1843 and 1275 proteins were identified in CC and oocytes, respectively, of which 86 and 54 were differentially regulated by PA. In CC, 20/86 proteins were mitochondrial, 16 of which were down-regulated. Canonical pathway analysis in CC showed that pro-apoptotic UPR, mitochondrial dysfunction, and other related redox regulatory, metabolic, and apoptotic pathways were the most affected. In the enclosed oocytes, 12/54 proteins were mitochondrial, 8 of which were up-regulated. Functional analysis of the DRP in oocytes suggests that pro-survival mechanisms were predominant. Mitochondrion-specific H2O2-scavenging enzyme (peroxiredoxin-3), mitochondrial trifunctional protein (HADHB), heat shock protein A8 (HSPA8), as well as the NRF2-mediated oxidative stress response, among others, were key regulatory mechanisms induced by PA in the oocytes. However, an increase in the relative abundance of cytochrome C was evident, which may trigger apoptosis. This was accompanied by up-regulation of SLC24A5, which negatively regulates mitochondrial outer membrane permeabilization and may prevent such apoptotic trigger. These data show that the mitochondria in oocytes, despite being structurally immature, regulate adaptive signalling pathways in response to metabolic stress. Although the proteomic changes in oocytes were predominantly anti-apoptotic, certain defective pro-apoptotic changes were identified. These data provide a unique insight into the mitochondrial adaptive signalling pathways in metabolically compromised oocytes, and indicate specific mitochondrial target pathways through which the developmental capacity of metabolically compromised oocytes can be improved or protected.