bluuebunny/crossref_metadata_2025 huggingface dataset:
Structural Statistical Quantifiers and Thermal Features of Quantum Systems
Authors: Pennini Flavia , Plastino Angelo , Plastino Angel Ricardo , Hernando Alberto
Published: December 2020

This paper deals primarily with relatively novel thermal quantifiers called disequilibrium and statistical complexity, whose role is growing in different disciplines of physics and other sciences. These quantifiers are called L. Ruiz, Mancini, and Calvet (LMC) quantifiers, following the initials of the three authors who advanced them. We wish to establish information-theoretical bridges between LMC structural quantifiers and (1) Thermal Heisenberg uncertainties ΔxΔp (at temperature T); (2) A nuclear physics fermion model. Having achieved such purposes, we determine to what an extent our bridges can be extended to both the semi-classical and classical realms. In addition, we find a strict bound relating a special LMC structural quantifier to quantum uncertainties.
A Survey of Candidate Waveforms for beyond 5G Systems
Authors: Conceição Filipe , Gomes Marco , Silva Vitor , Dinis Rui , Silva Adão , Castanheira Daniel
Published: December 2020

The 5G and beyond future wireless networks aim to support a large variety of services with increasing demand in terms of data rate and throughput while providing a higher degree of reliability, keeping the overall system complexity affordable. One of the key aspects regarding the physical layer architecture of such systems is the definition of the waveform to be used in the air interface. Such waveforms must be studied and compared in order to choose the most suitable and capable of providing the 5G and beyond services requirements, with flexible resource allocation in time and frequency domains, while providing high spectral and power efficiencies. In this paper, several beyond 5G waveforms candidates are presented, along with their transceiver architectures. Additionally, the associated advantages and disadvantages regarding the use of these transmission techniques are discussed. They are compared in a similar downlink transmission scenario where three main key performance indicators (KPIs) are evaluated. They are the peak-to-average power ratio, the overall system spectral efficiency (wherein the out of band emissions are measured, along with the spectral confinement of the power spectral density of the transmitted signals) and the bit error rate performance. Additionally, other KPIs are discussed.
Stacked Buck Converter: Current Ripple Elimination Effect and Transient Response
Authors: Huang Chien-Chun , Liu Yu-Chen , Lin Chia-Ching , Ni Chih-Yu , Chiu Huang-Jen
Published: December 2020

To balance the cost and volume when applying a low output current ripple, the power supply design should be able to eliminate the current ripple under any duty cycle in medium and high switching frequencies, and considerably reduce filter volume to improve power density. A stacked buck converter was eventually selected after reviewing the existing solutions and discussing their advantages and disadvantages. A stacked buck converter is used as a basis to propose the transient response and output current ripple elimination effect, boundary limit control method, and low output ripple dead time modulation method to make individual improvements. The principle, mathematical derivation, small-signal model, and compensator design method of the improvement method are presented in detail. Moreover, simulation results are used to mutually verify the correctness and effectiveness of the improvement method. A stacked buck converter with 330-V input, 50-V output, and 1-kW output power was implemented to verify the effect of the low output current ripple dead time modulation. Experimental results showed that the peak-to-peak value of the output current ripple was reduced from 2.09 A to 559 mA, and the RMS value was reduced from 551 mA to 91 mA, thereby effectively improving the output current ripple.
Performance Evaluation of a Stator Modular Ring Generator for a Shrouded Wind Turbine
Authors: Oliveira Jefferson A. , Filho Ály F. Flores
Published: December 2020

This paper presents the performance evaluation of a stator modular ring permanent-magnet generator to be embedded in a shrouded wind turbine. That is done to increase the power conversion for the same turbine area when compared to more conventional ones. An adapted structure allows the assembling of the prototype, aiming to verify its performance under controlled conditions. Aiming to verify the accuracy of an analytical subdomain model for a large diameter machine, the evaluation compares the results obtained by the electromagnetic finite element method and experimental measurements. The results of the components of the air-gap flux density, back EMF and electromagnetic torque obtained by the proposed analytical model and finite-element method are in good agreement with the experimental measurements. The experimental measurements of the iron loss and copper loss show that the prototype efficiency can reach 90% approximately.
Energy-Saving Inertial Drive for Dual-Frequency Excitation of Vibrating Machines
Authors: Gursky Volodymyr , Kuzio Igor , Krot Pavlo , Zimroz Radoslaw
Published: December 2020

The low energy efficiency and excessive power of electric motors of large-scale vibrating machines for processing bulk materials motivated a new design of the inertial drive. This drive consists of one motor and two coaxial unbalanced masses, whose rotational frequencies are related in the ratio 2:1. This approach allows for a generation of the excitation force with variable amplitude and frequency, which changes depending on the inertial characteristics and shaft rotation frequency and does not relate to the phase difference of the unbalanced masses. Because of this, the symmetry axis of the resulting vector hodograph can be changed. The spectral composition of the exciting force up to 200 Hz contains higher harmonics, the energy share of which is 25.4% from the 2nd harmonic and 14.1% from the 3rd and higher harmonics that correspondingly improves bulk material treatment in comparison to single-frequency vibrators. The finite element model is used for checking the strength capacity of the most loaded units of a dual-frequency drive. Its use allows the realization of complex trajectories of motion that are more technologically efficient for variable parameters of the treated media and energy saving in sieving screens and other vibrating machines.
Systematic Method for the Energy-Saving Potential Calculation of Air-Conditioning Systems via Data Mining. Part I: Methodology
Authors: Ma Rongjiang , Yang Shen , Wang Xianlin , Wang Xi-Cheng , Shan Ming , Yu Nanyang , Yang Xudong
Published: December 2020

Air-conditioning systems contribute the most to energy consumption among building equipment. Hence, energy saving for air-conditioning systems would be the essence of reducing building energy consumption. The conventional energy-saving diagnosis method through observation, test, and identification (OTI) has several drawbacks such as time consumption and narrow focus. To overcome these problems, this study proposed a systematic method for energy-saving diagnosis in air-conditioning systems based on data mining. The method mainly includes seven steps: (1) data collection, (2) data preprocessing, (3) recognition of variable-speed equipment, (4) recognition of system operation mode, (5) regression analysis of energy consumption data, (6) constraints analysis of system running, and (7) energy-saving potential analysis. A case study with a complicated air-conditioning system coupled with an ice storage system demonstrated the effectiveness of the proposed method. Compared with the traditional OTI method, the data-mining-based method can provide a more comprehensive analysis of energy-saving potential with less time cost, although it strongly relies on data quality in all steps and lacks flexibility for diagnosing specific equipment for energy-saving potential analysis. The results can deepen the understanding of the operating data characteristics of air-conditioning systems.
Food–Drug Interactions with Fruit Juices
Authors: Petric Zvonimir , Žuntar Irena , Putnik Predrag , Bursać Kovačević Danijela
Published: December 2020

Fruit juices contain a large number of phytochemicals that, in combination with certain drugs, can cause food–drug interactions that can be clinically significant and lead to adverse events. The mechanisms behind such interactions are in most cases related to phytochemical interference with the activity of cytochrome P450 metabolizing enzymes (CYPs) or drug transporters. Moreover, alterations in their activity can have a clinical relevance if systemic exposure to the drug is decreased or increased, meaning that the pharmacological drug effects are suboptimal, or the drug will cause toxicity. In general, the common pharmacokinetic parameters found to be altered in food–drug interactions regarding fruit juices are the area under the concentration–time curve, bioavailability, and maximum plasma concentration. In most cases, the results from the drug interaction studies with fruit juices provide only limited information due to the small number of subjects, which are also healthy volunteers. Moreover, drug interactions with fruit juices are challenging to predict due to the unknown amounts of the specific phytochemicals responsible for the interaction, as well as due to the inter-individual variability of drug metabolism, among others. Therefore, this work aims to raise awareness about possible pharmacological interactions with fruit juices.
Pomological, Sensorial, Nutritional and Nutraceutical Profile of Seven Cultivars of Cherimoya (Annona cherimola Mill)
Authors: Gentile Carla , Mannino Giuseppe , Palazzolo Eristanna , Gianguzzi Giuseppe , Perrone Anna , Serio Graziella , Farina Vittorio
Published: December 2020

In this work, the food quality of four international (Campas, Chaffey, Fino de Jete and White) and three local (Daniela, Torre1 and Torre2) cultivars of Cherimoya (Annona cherimola Mill) was investigated. With this aim, pomological traits, sensorial attributes, physiochemical parameters (pH, total soluble content and total acidity), nutritional composition (macro- and micro-nutrients) and nutraceutical values (bioactive compounds, radical scavenging and antioxidant properties) were evaluated. Among the seven observed cultivars, Fino de Jete was identified as the best, not only for its commercial attributes such as pomological traits and physiochemical values, but also for its nutritional composition. On the other hand, Chaffey and Daniela were the cultivars with the highest content of polyphenols, proanthocyanidins, and with the strongest antioxidant capacity. Concerning the two local ecotypes, Torre1 and Torre2, they displayed a balanced nutritional profile that, if combined with their discrete nutraceutical, physicochemical and pomological values, may result in a reassessment of their commercial impact. In conclusion, our data provide interesting information about the pomological, nutritional, and nutraceutical properties of cherimoya fruits. Our results, in addition to promoting the commercial impact of local cultivars, may increase the use of individual cultivars in breeding programs.
Proximate Chemical Composition, Fatty Acid Profile, and Lipid Qualitative Indices of Brown Bear Meat
Authors: Kelava Ugarković Nikolina , Konjačić Miljenko , Malnar Josip , Tomljanović Kristijan , Šprem Nikica , Ugarković Damir
Published: December 2020

Although game meat quality has been under the spotlight in numerous studies, the quality of brown bear (Ursus arctos) meat is still unknown. The aim of this study was to determine the effects of sex and age on the proximate chemical composition, fatty acid profile, and lipid indices of brown bear meat. Nine (n = 9) females and nine (n = 9) males were hunted during the Croatian spring hunting period in 2018. Based on age, bears were divided into two groups: <3 years (n = 9; five females and four males) and 4–6 years (n = 9; four females and five males). For analysis purposes, samples of M.semimembranosus were collected. Age was shown to have an effect on the traits analyzed, while sex-related differences were not found. Brown bear meat has a high fat content (average 6.12%), especially in older bears (~9%). The contents of protein, dry matter, and ash were similar to those of other game species. Monounsaturated fatty acids made up approximately 50% of all fatty acids, with the most abundant being C18:1n-9. More favorable profiles of essential polyunsaturated fatty acids were found in younger bears. The ratio of polyunsaturated and saturated fatty acids was closer to the recommended ratio than the ratio of n-6 and n-3 polyunsaturated fatty acids, and lipid indices were favorable. Further research is needed to determine seasonal changes in brown bear meat quality.
Food Bioactive Compounds and Emerging Techniques for Their Extraction: Polyphenols as a Case Study
Authors: Câmara José S. , Albuquerque Bianca R. , Aguiar Joselin , Corrêa Rúbia C. G. , Gonçalves João L. , Granato Daniel , Pereira Jorge A. M. , Barros Lillian , Ferreira Isabel C. F. R.
Published: December 2020

Experimental studies have provided convincing evidence that food bioactive compounds (FBCs) have a positive biological impact on human health, exerting protective effects against non-communicable diseases (NCD) including cancer and cardiovascular (CVDs), metabolic, and neurodegenerative disorders (NDDs). These benefits have been associated with the presence of secondary metabolites, namely polyphenols, glucosinolates, carotenoids, terpenoids, alkaloids, saponins, vitamins, and fibres, among others, derived from their antioxidant, antiatherogenic, anti-inflammatory, antimicrobial, antithrombotic, cardioprotective, and vasodilator properties. Polyphenols as one of the most abundant classes of bioactive compounds present in plant-based foods emerge as a promising approach for the development of efficacious preventive agents against NCDs with reduced side effects. The aim of this review is to present comprehensive and deep insights into the potential of polyphenols, from their chemical structure classification and biosynthesis to preventive effects on NCDs, namely cancer, CVDs, and NDDS. The challenge of polyphenols bioavailability and bioaccessibility will be explored in addition to useful industrial and environmental applications. Advanced and emerging extraction techniques will be highlighted and the high-resolution analytical techniques used for FBCs characterization, identification, and quantification will be considered.
Emulsion and Surface-Active Properties of Fish Solubles Based on Direct Extraction and after Hydrolysis of Atlantic Cod and Atlantic Salmon Backbones
Authors: Steinsholm Silje , Oterhals Åge , Underhaug Jarl , Aspevik Tone
Published: December 2020

The focus on natural foods and “clean” labeled products is increasing and encourages development of new biobased ingredients. Fish solubles derived from downstream processing of side stream materials in the fish filleting industries have potential as emulsifiers based on their surface-active and emulsion stabilizing properties. The aim of this study was to evaluate and compare emulsion properties and critical micelle concentration (CMC) of direct protein extracts and protein hydrolysates based on fish backbones, and to identify associations between molecular weight distribution and process yield with the studied physicochemical properties. Protein extracts and enzymatic protein hydrolysates were produced based on two raw materials (cod and salmon backbones), two enzymes with different proteolytic specificity, and varying hydrolysis time. Emulsion activity index (EAI), emulsion stability index (ESI) and CMC were measured and compared with casein as a reference to protein-based emulsifiers. Protein hydrolysis was found to have negative impact on EAI and CMC, likely due to generation of small peptides disrupting the amphiphilic balance. The direct protein extracts had comparable EAI with casein, but the latter had superior ESI values. Protein hydrolysates with acceptable EAI could only be obtained at the expense of product yield. The study emphasizes the complexity of physicochemical properties of protein hydrolysates and discusses the challenges of achieving both good surface-active properties and high product yield.
Animal and Plant Protein Oxidation: Chemical and Functional Property Significance
Authors: Xiong Youling L. , Guo Anqi
Published: December 2020

Protein oxidation, a phenomenon that was not well recognized previously but now better understood, is a complex chemical process occurring ubiquitously in food systems and can be induced by processing treatments as well. While early research concentrated on muscle protein oxidation, later investigations included plant, milk, and egg proteins. The process of protein oxidation involves both radicals and nonradicals, and amino acid side chain groups are usually the site of initial oxidant attack which generates protein carbonyls, disulfide, dityrosine, and protein radicals. The ensuing alteration of protein conformational structures and formation of protein polymers and aggregates can result in significant changes in solubility and functionality, such as gelation, emulsification, foaming, and water-holding. Oxidant dose-dependent effects have been widely reported, i.e., mild-to-moderate oxidation may enhance the functionality while strong oxidation leads to insolubilization and functionality losses. Therefore, controlling the extent of protein oxidation in both animal and plant protein foods through oxidative and antioxidative strategies has been of wide interest in model system as well in in situ studies. This review presents a historical perspective of food protein oxidation research and provides an inclusive discussion of the impact of chemical and enzymatic oxidation on functional properties of meat, legume, cereal, dairy, and egg proteins based on the literature reports published in recent decades.
In-Bag Dry- vs. Wet-Aged Lamb: Quality, Consumer Acceptability, Oxidative Stability and In Vitro Digestibility
Authors: Zhang Renyu , Yoo Michelle J. Y. , Realini Carolina E. , Staincliffe Maryann , Farouk Mustafa M.
Published: December 2020

The aim of this study was to produce in-bag dry-aged lamb and compare its meat quality, consumer acceptability, oxidative stability and in vitro digestibility to the wet-aged equivalents. Significantly higher pH, weight loss and reduced cook loss were observed in dry-aged lamb compared to the wet-aged (p < 0.0001). Dry-aged lamb had harder and chewier texture profiles and lower colour attributes (L*, a* and b*) than the wet-aged (p < 0.001). The dry-aged and wet-aged lamb were equally preferred (around 40% each) by the consumer panel, underpinning the niche nature of dry-aged meat. Significantly (p < 0.05) higher yeast and thiobarbituric acid reactive substances (TABRS) levels were observed in dry-aged lamb compared to the wet-aged. There was no difference in fatty acid profile, protein carbonyl content and pattern of proteolysis between ageing regimes (p > 0.05). Ageing regimes had no impact on overall digestibility; however, a greater gastric digestibility was observed in dry-aged lamb through the increased release of free amino acids (FAAs) compared to the wet-aged. Outcomes of this study demonstrated for the first time the possibility of producing dry-aged lamb legs of acceptable quality, oxidative stability and superior digestibility compared to the equivalent wet-aged lamb.
New Blacks: Language, DNA, and the Construction of the African American/Dominican Boundary of Difference
Authors: Clemons Aris Moreno
Published: December 2020

Given the current political climate in the U.S.—the civil unrest regarding the recognition of the Black Lives Matter movement, the calls to abolish prisons and U.S. Immigration and Customs Enforcement (ICE) detention, and the workers’ rights movements—projects investigating moments of inter-ethnic solidarity and conflict remain essential. Because inter-ethnic conflict and solidarity in communities of color have become more visible as waves of migration over the past 50 years have complicated and enriched the sociocultural landscape of the U.S., I examine the ways that raciolinguistic ideologies are reflected in assertions of ethno-racial belonging for Afro-Dominicans and their descendants. Framing my analysis at the language, race, and identity interface, I ask what mechanisms are used to perform Blackness and/or anti-Blackness for Dominican(-American)s and in what ways does this behavior contribute to our understanding of Blackness in the U.S.? I undertake a critical discourse analysis on 10 YouTube videos that discuss what I call the African American/Dominican boundary of difference. The results show that the primary inter-ethnic conflict between Dominican(-Americans) and African Americans was posited through a categorization fallacy, in which the racial term “Black” was conceived as an ethnic term for use only with African Americans.
Modeling and Simulation of Tsunami Impact: A Short Review of Recent Advances and Future Challenges
Authors: Marras Simone , Mandli Kyle T.
Published: December 2020

Tsunami modeling and simulation has changed in the past few years more than it has in decades, especially with respect to coastal inundation. Among other things, this change is supported by the approaching era of exa-scale computing, whether via GPU or more likely forms of hybrid computing whose presence is growing across the geosciences. For reasons identified in this review, exa-scale computing efforts will impact the on-shore, highly turbulent régime to a higher degree than the 2D shallow water equations used to model tsunami propagation in the open ocean. This short review describes the different approaches to tsunami modeling from generation to impact and underlines the limits of each model based on the flow régime. Moreover, from the perspective of a future comprehensive multi-scale modeling infrastructure to simulate a full tsunami, we underline the current challenges associated with this approach and review the few efforts that are currently underway to achieve this goal. A table of existing tsunami software packages is provided along with an open Github repository to allow developers and model users to update the table with additional models as they are published and help with model discoverability.
Predictors of Health-Related Quality of Life among Healthcare Workers in the Context of Health System Strengthening in Kenya
Authors: Muthuri Rose Nabi Deborah Karimi , Senkubuge Flavia , Hongoro Charles
Published: December 2020

Kenya is among the countries with an acute shortage of skilled health workers. There have been recurrent health worker strikes in Kenya due to several issues, some of which directly or indirectly affect their health. The purpose of this study was to investigate the predictors of health-related quality of life (HRQOL) among healthcare workers in public and mission hospitals in Meru County, Kenya. A cross-sectional study design was undertaken among 553 healthcare workers across 24 hospitals in Meru County. The participants completed the EuroQol-five dimension-five level (EQ-5D-5L) instrument, which measures health status across five dimensions and the overall self-assessment of health status on a visual analogue scale (EQ-VAS). Approximately 66.55% of the healthcare workers reported no problems (i.e., 11,111) across the five dimensions. The six predictors of HRQOL among the healthcare workers were hospital ownership (p < 0.05), age (p < 0.05), income (p < 0.01), availability of water for handwashing (p < 0.05), presence of risk in using a toilet facility (p < 0.05), and overall safety of hospital work environment (p < 0.05). Personal, job-related attributes and work environment characteristics are significant predictors of healthcare workers HRQOL. Thus, these factors ought to be considered by health policymakers and managers when developing and implementing policies and programs aimed at promoting HRQOL among healthcare workers.
The Activity and Stability of p56Lck and TCR Signaling Do Not Depend on the Co-Chaperone Cdc37
Authors: Kowallik Sarah , Kritikos Andreas , Kästle Matthias , Thurm Christoph , Schraven Burkhart , Simeoni Luca
Published: December 2020

Lymphocyte-specific protein tyrosine kinase (Lck) is a pivotal tyrosine kinase involved in T cell receptor (TCR) signaling. Because of its importance, the activity of Lck is regulated at different levels including phosphorylation of tyrosine residues, protein–protein interactions, and localization. It has been proposed that the co-chaperone Cdc37, which assists the chaperone heat shock protein 90 (Hsp90) in the folding of client proteins, is also involved in the regulation of the activity/stability of Lck. Nevertheless, the available experimental data do not clearly support this conclusion. Thus, we assessed whether or not Cdc37 regulates Lck. We performed experiments in which the expression of Cdc37 was either augmented or suppressed in Jurkat T cells. The results of our experiments indicated that neither the overexpression nor the suppression of Cdc37 affected Lck stability and activity. Moreover, TCR signaling proceeded normally in T cells in which Cdc37 expression was either augmented or suppressed. Finally, we demonstrated that also under stress conditions Cdc37 was dispensable for the regulation of Lck activity/stability. In conclusion, our data do not support the idea that Lck is a Cdc37 client.
Molecular Mechanisms of Colon Cancer Progression and Metastasis: Recent Insights and Advancements
Authors: Malki Ahmed , ElRuz Rasha Abu , Gupta Ishita , Allouch Asma , Vranic Semir , Al Moustafa Ala-Eddin
Published: December 2020

Colorectal cancer (CRC), the third most common type of cancer, is the second leading cause of cancer-related mortality rates worldwide. Although modern research was able to shed light on the pathogenesis of CRC and provide enhanced screening strategies, the prevalence of CRC is still on the rise. Studies showed several cellular signaling pathways dysregulated in CRC, leading to the onset of malignant phenotypes. Therefore, analyzing signaling pathways involved in CRC metastasis is necessary to elucidate the underlying mechanism of CRC progression and pharmacotherapy. This review focused on target genes as well as various cellular signaling pathways including Wnt/β-catenin, p53, TGF-β/SMAD, NF-κB, Notch, VEGF, and JAKs/STAT3, which are associated with CRC progression and metastasis. Additionally, alternations in methylation patterns in relation with signaling pathways involved in regulating various cellular mechanisms such as cell cycle, transcription, apoptosis, and angiogenesis as well as invasion and metastasis were also reviewed. To date, understanding the genomic and epigenomic instability has identified candidate biomarkers that are validated for routine clinical use in CRC management. Nevertheless, better understanding of the onset and progression of CRC can aid in the development of early detection molecular markers and risk stratification methods to improve the clinical care of CRC patients.
Possible Receptor Mechanisms Underlying Cannabidiol Effects on Addictive-like Behaviors in Experimental Animals
Authors: Galaj Ewa , Xi Zheng-Xiong
Published: December 2020

Substance use disorder (SUD) is a serious public health problem worldwide for which available treatments show limited effectiveness. Since the legalization of cannabis and the approval of cannabidiol (CBD) by the US Food and Drug Administration, therapeutic potential of CBD for the treatment of SUDs and other diseases has been widely explored. In this mini-review article, we first review the history and evidence supporting CBD as a potential pharmacotherapeutic. We then focus on recent progress in preclinical research regarding the pharmacological efficacy of CBD and the underlying receptor mechanisms on addictive-like behavior. Growing evidence indicates that CBD has therapeutic potential in reducing drug reward, as assessed in intravenous drug self-administration, conditioned place preference and intracranial brain-stimulation reward paradigms. In addition, CBD is effective in reducing relapse in experimental animals. Both in vivo and in vitro receptor mechanism studies indicate that CBD may act as a negative allosteric modulator of type 1 cannabinoid (CB1) receptor and an agonist of type 2 cannabinoid (CB2), transient receptor potential vanilloid 1 (TRPV1), and serotonin 5-HT1A receptors. Through these multiple-receptor mechanisms, CBD is believed to modulate brain dopamine in response to drugs of abuse, leading to attenuation of drug-taking and drug-seeking behavior. While these findings suggest that CBD is a promising therapeutic candidate, further investigation is required to verify its safety, pharmacological efficacy and the underlying receptor mechanisms in both experimental animals and humans.
Regional Differences in Neuroinflammation-Associated Gene Expression in the Brain of Sporadic Creutzfeldt–Jakob Disease Patients
Authors: Areškevičiūtė Aušrinė , Litman Thomas , Broholm Helle , Melchior Linea C. , Nielsen Pia R. , Green Alison , Eriksen Jens O. , Smith Colin , Lund Eva L.
Published: December 2020

Neuroinflammation is an essential part of neurodegeneration. Yet, the current understanding of neuroinflammation-associated molecular events in distinct brain regions of prion disease patients is insufficient to lay the ground for effective treatment strategies targeting this complex neuropathological process. To address this problem, we analyzed the expression of 800 neuroinflammation-associated genes to create a profile of biological processes taking place in the frontal cortex and cerebellum of patients who suffered from sporadic Creutzfeldt–Jakob disease. The analysis was performed using NanoString nCounter technology with human neuroinflammation panel+. The observed gene expression patterns were regionally and sub-regionally distinct, suggesting a variable neuroinflammatory response. Interestingly, the observed differences could not be explained by the molecular subtypes of sporadic Creutzfeldt–Jakob disease. Furthermore, analyses of canonical pathways and upstream regulators based on differentially expressed genes indicated an overlap between biological processes taking place in different brain regions. This suggests that even smaller-scale spatial data reflecting subtle changes in brain cells’ functional heterogeneity and their immediate pathologic microenvironments are needed to explain the observed differential gene expression in a greater detail.
Extracellular Vesicles from Red Blood Cells and Their Evolving Roles in Health, Coagulopathy and Therapy
Authors: Thangaraju Kiruphagaran , Neerukonda Sabari Nath , Katneni Upendra , Buehler Paul W.
Published: December 2020

Red blood cells (RBCs) release extracellular vesicles (EVs) including both endosome-derived exosomes and plasma-membrane-derived microvesicles (MVs). RBC-derived EVs (RBCEVs) are secreted during erythropoiesis, physiological cellular aging, disease conditions, and in response to environmental stressors. RBCEVs are enriched in various bioactive molecules that facilitate cell to cell communication and can act as markers of disease. RBCEVs contribute towards physiological adaptive responses to hypoxia as well as pathophysiological progression of diabetes and genetic non-malignant hematologic disease. Moreover, a considerable number of studies focus on the role of EVs from stored RBCs and have evaluated post transfusion consequences associated with their exposure. Interestingly, RBCEVs are important contributors toward coagulopathy in hematological disorders, thus representing a unique evolving area of study that can provide insights into molecular mechanisms that contribute toward dysregulated hemostasis associated with several disease conditions. Relevant work to this point provides a foundation on which to build further studies focused on unraveling the potential roles of RBCEVs in health and disease. In this review, we provide an analysis and summary of RBCEVs biogenesis, composition, and their biological function with a special emphasis on RBCEV pathophysiological contribution to coagulopathy. Further, we consider potential therapeutic applications of RBCEVs.
Pharmacogenomics, How to Deal with Different Types of Variants in Next Generation Sequencing Data in the Personalized Medicine Area
Authors: Tafazoli Alireza , Wawrusiewicz-Kurylonek Natalia , Posmyk Renata , Miltyk Wojciech
Published: December 2020

Pharmacogenomics (PGx) is the knowledge of diverse drug responses and effects in people, based on their genomic profiles. Such information is considered as one of the main directions to reach personalized medicine in future clinical practices. Since the start of applying next generation sequencing (NGS) methods in drug related clinical investigations, many common medicines found their genetic data for the related metabolizing/shipping proteins in the human body. Yet, the employing of technology is accompanied by big obtained data, which most of them have no clear guidelines for consideration in routine treatment decisions for patients. This review article talks about different types of NGS derived PGx variants in clinical studies and try to display the current and newly developed approaches to deal with pharmacogenetic data with/without clear guidelines for considering in clinical settings.
In Vitro and In Vivo Models of Non-Alcoholic Fatty Liver Disease: A Critical Appraisal
Authors: Soret Pierre-Antoine , Magusto Julie , Housset Chantal , Gautheron Jérémie
Published: December 2020

Non-alcoholic fatty liver disease (NAFLD), including non-alcoholic fatty liver (NAFL) and non-alcoholic steatohepatitis (NASH), represents the hepatic manifestation of obesity and metabolic syndrome. Due to the spread of the obesity epidemic, NAFLD is becoming the most common chronic liver disease and one of the principal indications for liver transplantation. However, no pharmacological treatment is currently approved to prevent the outbreak of NASH, which leads to fibrosis and cirrhosis. Preclinical research is required to improve our knowledge of NAFLD physiopathology and to identify new therapeutic targets. In the present review, we summarize advances in NAFLD preclinical models from cellular models, including new bioengineered platforms, to in vivo models, with a particular focus on genetic and dietary mouse models. We aim to discuss the advantages and limits of these different models.
Exercise Induced Worsening of Mechanical Heterogeneity and Diastolic Impairment in Long QT Syndrome
Authors: Charisopoulou Dafni , Koulaouzidis George , Law Lucy F. , Rydberg Annika , Henein Michael Y.
Published: December 2020

Background: Electromechanical heterogeneities due to marked dispersion of ventricular repolarisation and mechanical function have been associated with symptoms in long QT syndrome (LQTS) patients; Aim: To examine the exercise response of longitudinal LV systolic and diastolic myocardial function and synchronicity in LQTS patients and its relationship with symptoms; Methods: Forty seven (age 45 ± 15 yrs, 25 female, 20 symptomatic) LQTS patients and 35 healthy individuals underwent an exercise test (Bruce protocol). ECG and echo parameters were recorded at rest, peak exercise (p.e.), and recovery; Results: LQTS patients had prolonged and markedly dispersed myocardial contraction, delayed early relaxation phase, and significantly decreased filling time at all exercise phases. Unlike controls, these electromechanical disturbances deteriorated further with exercise, during which additional decrease of the LV diastolic myocardial function and attenuated LV stroke volume were noted. Such abnormal responses to exercise were seen to a greater degree in symptomatic patients and in the LQT1 subgroup and improved with B-blocker therapy. Worsening myocardial contraction dispersion at p.e. was the strongest discriminator for previous clinical events, and its discriminating power excelled further by adding early relaxation delay; Conclusions: Electromechanical disturbances were shown to worsen during exercise in LQTS patients and were more pronounced in those with previous arrhythmic events.
Proposed Criteria for Constipation in Palliative Care Patients. A Multicenter Cohort Study
Authors: Dzierżanowski Tomasz , Larkin Philip
Published: December 2020

Although constipation is one of the most frequently reported symptoms in palliative care, there is no widely accepted definition of constipation and none suitable for patients unable to self-report or express symptoms. This study aimed to verify the objective and subjective symptoms of constipation to develop a diagnostic algorithm for constipation, which is also feasible in unconscious patients. In a pooled analysis of two observational studies, 369 out of 547 adult end-stage cancer patients met the inclusion criteria. The patient-reported difficulty of defecation correlated with observable measures, such as days since last bowel movement, and frequency of bowel movements. Difficulty became at least moderate when there were no bowel movements for ≥2 days, or the frequency of bowel movements was ≤3 per week. The diagnostic algorithm, comprising these three symptoms offers a simple, rapid, and comprehensive tool for palliative care, independent of the patient’s state of consciousness. A clinical trial is necessary to confirm its validity and usefulness.
Microscopic Peritoneal Residual Disease after Complete Macroscopic Cytoreductive Surgery for Advanced High Grade Serous Ovarian Cancer
Authors: Azaïs Henri , Vignion-Dewalle Anne-Sophie , Carrier Marine , Augustin Jeremy , Da Maïa Elisabeth , Penel Alix , Belghiti Jérémie , Nikpayam Marianne , Gonthier Clémentine , Ziane Laurine , Mordon Serge , Collinet Pierre , Canlorbe Geoffroy , Uzan Catherine
Published: December 2020

Background: Epithelial ovarian cancers (EOC) are usually diagnosed at an advanced stage and managed by complete macroscopic cytoreductive surgery (CRS) and systemic chemotherapy. Peritoneal recurrence occurs in 60% of patients and may be due to microscopic peritoneal metastases (mPM) which are neither eradicated by surgery nor controlled by systemic chemotherapy. The aim of this study was to assess and quantify the prevalence of residual mPM after complete macroscopic CRS in patients with advanced high-grade serous ovarian cancer (HGSOC). Methods: A prospective study conducted between 1 June 2018 and 10 July 2019 in a single referent center accredited by the European Society of Gynecological Oncology for advanced EOC management. Consecutive patients presenting with advanced HGSOC and eligible for complete macroscopic CRS were included. Up to 13 peritoneal biopsies were taken from macroscopically healthy peritoneum at the end of CRS and examined for the presence of mPM. A mathematical model was designed to determine the probability of presenting at least one mPM after CRS. Results: 26 patients were included and 26.9% presented mPM. There were no differences in characteristics between patients with or without identified mPM. After mathematical analysis, the probability that mPM remained after complete macroscopic CRS in patients with EOC was 98.14%. Conclusion: Microscopic PM is systematically present after complete macroscopic CRS for EOC and could be a relevant therapeutic target. Adjuvant locoregional strategies to conventional surgery may improve survival by achieving microscopic CRS.
Alexithymia in Alzheimer’s Disease
Authors: Arroyo-Anlló Eva Mª , Souchaud Corinne , Ingrand Pierre , Chamorro Sánchez Jorge , Melero Ventola Alejandra , Gil Roger
Published: December 2020

Alexithymia is widely recognized as the inability to identify and express emotions. It is a construct which consists of four cognitive traits such as difficulty in identifying feelings, describing feelings to others, externally oriented thinking, and limited imaginative capacity. Several studies have linked alexithymia to cognitive functioning, observing greater alexithymia scores associated with poorer cognitive abilities. Despite Alzheimer’s disease (AD) being a neurodegenerative pathology characterized by cognitive troubles from the early stages, associated to behavioral and emotional disturbances, very few investigations have studied the alexithymia in AD. These studies have shown that alexithymia scores—assessed with Toronto Alexithymia Scale (TAS)—were greater in AD patients than healthy participants. The objective of the study was to investigate if the alexithymia was present in patients with mild AD. We hypothesized that the AD group would show more alexithymia features than the control group. We evaluated 54 subjects, including 27 patients diagnosed with mild AD and 27 normal healthy controls, using the Shalling Sifneos Psychosomatic Scale (SSPS-R) and a neuropsychological test battery. Using non-parametric statistical analyses—Wilcoxon and Mann–Whitney U tests—we observed that the SSPS-R scores were similar in the AD and control groups. All participants showed SSPS-R scores below to 10 points, which means no-alexithymia. We did not find significant correlations between SSPS-R scores and cognitive variables in both groups (p > 0.22), but we observed a negative association between name abilities and alexithymia, but it does not reach to significance (p = 0.07). However, a significant correlation between SSPS-R score and mood state, assessed using Zerssen Rating Scale, was found in both groups (p = 0.01). Because we did not find a significant difference in the alexithymia assessment between both subject groups, pot hoc analyses were computed for each item of the SSPS-R. We made comparisons of alexithymic responses percentages in each SSPS-R item between AD and control groups, using Fisher’s test. We observed that AD patients produced more alexithymic responses in some items of SSPS-R test than the control group, particularly about difficulties to find the words to describe feelings, as well as difficulties of imagination capacity and externally oriented thinking. The present results do not confirm our hypothesis and they do not support the results of previous studies revealing great alexithymia in AD.
Comparison of Retinal Layer Thickness and Capillary Vessel Density in the Patients with Spontaneously Resolved Acute Central Serous Chorioretinopathy
Authors: Han Kyu Jin , Kim Hyeong Ju , Woo Je Moon , Min Jung Kee
Published: December 2020

We investigate retinal layer thickness and capillary vessel density (VD) in the patients with central serous chorioretinopathy (CSC) who recovered spontaneously and evaluate the correlation between the changes in these values and visual outcomes using swept-source optical coherence tomography (SS-OCT) and OCT angiography (OCTA). This retrospective case–control study included 34 eyes of 34 patients with spontaneously resolved acute CSC. The changes in retinal layer thickness and capillary VD were examined using SS-OCT and OCTA after complete resolution of subretinal fluid (SRF). The fellow eyes and 34 healthy eyes were used as controls. In the eyes with CSC, the outer retinal layer was significantly thinner than in the eyes of fellow and healthy controls. The foveal avascular zone area and VDs in the superficial and deep capillary plexus in the eyes with CSC were not significantly different from those in the eyes of fellow and healthy controls. The VD of the choriocapillaris in the eyes with CSC was significantly lower than that in the eyes of fellow and healthy controls. Correlation analyses revealed that the outer retinal layer thickness and initial visual acuity were positively correlated with the final visual acuity. Furthermore, the initial SRF area and height were negatively correlated with the outer retinal layer thickness after SRF resolution. Attenuation of outer retinal layer thickness and decreased VD of the choriocapillaris were observed in the eyes with spontaneously resolved acute CSC. The outer retinal layer thickness could be an important visual predictor of CSC.
A Gap in Brain Gain for Emerging Countries: Evidence of International Immigration on Non-Resident Patents
Authors: Enkhtaivan Bolortuya , Brusa Jorge , Davaadorj Zagdbazar
Published: December 2020

Immigration is a controversial topic that draws much debate. From a human sustainability perspective, immigration is disadvantageous for home countries causing brain drains. Ample evidence suggests the developed host countries benefit from immigration in terms of diversification, culture, learning, and brain gains, yet less is understood for emerging countries. The purpose of this paper is to examine the presence of brain gains due to immigration for emerging countries, and explore any gaps as compared to developed countries. Using global data from 88 host and 109 home countries over the period from 1995 to 2015, we find significant brain gains due to immigration for emerging countries. However, our results show that there is still a significant brain gain gap between emerging and developed countries. A brain gain to the developed host countries is about 5.5 times greater than that of the emerging countries. The results hold after addressing endogeneity, self-selection, and large sample biases. Furthermore, brain gain is heterogenous by immigrant types. Skilled or creative immigrants tend to benefit the host countries about three times greater than the other immigrants. In addition, the Top 10 destination countries seem to attract the most creative people, thus harvest the most out of the talented immigrants. In contrast, we find countries of origin other than the Top 10 seem to send these creative people to the rest of the world.
Assessing the Bonitet of Cadastral Parcels for Land Reallocation in Urban Consolidation
Authors: Kilić Pamuković Jelena , Rogulj Katarina , Jajac Nikša
Published: December 2020

Cadastral parcels valuation is a very important aspect when defining the priorities for the selection of newly formed parcels in the reallocation processes. Land reallocation involves a new redistribution of land parcels in the project implementation area. In doing so, it is necessary to take into account the preferences of private owners, the value of cadastral parcels, and their bonitet values, whose determination is the basic task of this research. The main goal is to propose a model to assess the bonitet of private cadastral parcels based on Expert System (ES) of fuzzy logic within the knowledge component, which would reduce uncertainty and increase the objectivity of the evaluation. Expert knowledge is included in the evaluation process by defining weighting coefficients for optimizing the rule base, and linear and nonlinear value functions for criteria standardizing. By applying the newly formed ES in the bonitet assessment, with the created base of expert knowledge, the processes of estimating attribute values have been improved, especially in the form of reducing uncertainty in the assessment of urban land parcels, as well as increasing objectivity by involving a group of experts in the model creation. The proposed model also provides equal access to all stakeholders in the process of urban renewal with different requirements and desires. The model also provides support in conducting the negotiation procedures and planning of land reallocation implementation. The proposed model was tested on the field of the construction of the Campus of University of Split.