bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization of multi-wavelength polarized light transmission in the real sea spray environment
Authors: Luo Kaiming , Fu Qiang , Zhang Yue , Yang Wei , Zhang Su , Zhan Juntong , Li Zheng , Ma Yi , Duan Jin , Jiang Huilin
Published: September 2023

Sea spray particles are a type of non-uniform, non-spherical, non-isotropic, and complex medium, and the study of the transmission characteristics of polarized light in a real sea spray environment can provide reference values in many fields, such as polarization imaging, marine target detection, and LiDAR, which can make up for the vacancy of polarized light transmission in a complex sea spray environment. In this paper, a real sea fog test is carried out in the Qingdao Sea area of China in the horizontal/oblique direction, and a platform for generating and detecting polarized light with multiple tilt angles is constructed by using the active test method, which realizes the test scheme for the characteristics of energy change and polarization state change in the linearly polarized light and circularly polarized light at different visibility levels in sea fog environments. The results show that it is more difficult to deflect the circularly polarized light than linearly polarized light at the same sea spray visibility level. With the increase in the tilt angle, a decrease in the polarization is observed. The polarization of the near-infrared light is always larger than that of the visible light, which indicates that the circularly polarized light has better polarization preservation than the linearly polarized light and the polarization preservation of the near-infrared light is better than that of the visible light.
Application of a nerve stereoscopic reconstruction technique based on ultrasonic images in the diagnosis of neuralgic amyotrophy
Authors: Yu Miao , Ding Wenquan , Shao Guoqing , Li Miaozhong , Zhou Xiaoling , Liu Linhai , Li Xueyuan
Published: September 2023

Objective: To propose a nerve stereoscopic reconstruction technique based on ultrasound imaging for site diagnosis, intuitive reflection of disease severity, and classification of neuralgic amyotrophy (NA).

Methods: We enrolled 44 patients with NA who underwent high-frequency ultrasonography examination. Multiple sites on the normal side and the affected side were scanned to calculate the ratio of the cross-section area (CSA) of the affected side to the normal side at each location measured, i.e., the cross-section area swelling ratio (CSASR). The CSASR of 44 patients and 30 normal controls was analyzed to determine their threshold value for the diagnosis of NA. Then, ultrasound images of the cross-section were used to reconstruct the stereoscopic model of the nerve on the affected side and the normal side. Using the CSASR values in each measurement location, a CSASR stereoscopic model was developed.

Results: The threshold value of CSASR for ultrasound diagnosis of NA was 1.55. The average diseased segments per patient was 2.49 ± 1.97, with an average overall length of 10.03 ± 7.95 cm. Nerve stereoscopic reconstruction could be conducted for swelling, torsion, incomplete constriction, and complete constriction.

Conclusion: The ultrasound image reconstruction method proposed in this study can accurately determine the site, range, and type of neuropathies in patients with NA, and simultaneously provide complete and accurate data information and intuitive morphological information.
An evaluation based on the analytic hierarchy process and GGEbiplot on French fry potato genotypes in Yunnan, China
Authors: Liang Shumin , Jiang Wei , Yang Yan , Lu Lili , Whitworth Jonathan L. , Novy Richard G. , Bao Lixian , Yin Ziyou , Li Zhou , He Pinggen , Xu Youxian , Li Xianping
Published: September 2023

A total of 33 potato (Solanum tuberosum L.) cultivars and breeding clones imported from the United States and two local cultivars (Yunshu 401 and Cooperation 88, CK) were planted and evaluated. To determine their suitability for processing into French fries at five locations (e1-e5) in Yunnan Province, China, we developed a comprehensive evaluation system using the analytical hierarchy process (AHP). Eleven evaluation indicators for French fry quality, yield, and agronomic characteristics with a relative importance (weight coefficients) of 0.483, 0.301 and 0.216, respectively, were used to analyze the 35 potato genotypes (designated g1-g35).The genotypes were ranked and the results revealed that (1) on the average, the 33 potato genotypes imported from the United States showed a lower performance compared to the local cultivars. Compared with the CK, they were classified as not vigorous (Mean 5.11 vs CK 7.75), matured earlier (Mean 5.79 vs CK 1.70), and had a low resistance to late blight (Mean 3735.59 vs CK 1418.55), requiring the use of fungicides to control the disease at the five trial locations. (2) The US cultivar ‘Defender’ (g3) ranked in the top six at all five test locations because it had higher yield (29.56 t h m-2), better fry quality (4.64), higher dry matter content (20.41%), better tuber length/width ratio (1.99), longer tubers (13.57cm), stronger plant vigor (7.17) and higher resistance to late blight (AUDPC: 3134.2). (3) By using GGEbiplot analysis, superior genotypes with high and stable yields were g3 and ‘Echo Russet’ (g33). ‘Yunshu 401’ (g34) and ‘Yukon Gem’ (g4) had high but not stable yields. The ideal test environments and hence experimental locations were Luquan (LQ, e2) and Lijiang (LJ, e4) which resulted in the best discrimination between genotypes among the five experimental locations in Yunnan. Overall, the developed evaluation system based on AHP and GGEbiplot analysis including 11 evaluation indicators for French fry quality, yield and agricultural traits can be a model for evaluation and promotion of new French fry cultivars, and evaluating and selecting the test location.
Vigour testing for the rice seed with computer vision-based techniques
Authors: Qiao Juxiang , Liao Yun , Yin Changsheng , Yang Xiaohong , Tú Hoàng Minh , Wang Wei , Liu Yanfang
Published: September 2023

Rice is the staple food for approximately half of the world’s population. Seed vigour has a crucial impact on the yield, which can be evaluated by germination rate, vigor index and etc. Existing seed vigour testing methods heavily rely on manual inspections that are destructive, time-consuming, and labor-intensive. To address the drawbacks of existing rice seed vigour testing, we proposed a multispectral image-based non-destructive seed germination testing approach. Specifically, we collected multispectral data in 19 wavebands for six rice varieties. Furthermore, we designed an end-to-end pipeline, denoted as MsiFormer (MisFormer cod3e will be available at https://github.com/LiaoYun0x0/MisFormer) by integrating a Yolo-based object detector (trained Yolo v5) and a vision transformer-based vigour testing model, which effectively improved the automation and efficiency of existing techniques. In order to objectively evaluate the performance of the proposed method in this paper, we conduct a comparison between MisFormer and other 3 deep learning methods. The results showed that, MisFormer performed much better with the accuracy of 94.17%, which was 2.5%-18.34% higher than the other 3 deep learning methods. Besides MsiFormer, possibilities of CIELab mediated image analysis of TTC (tetrazolium chloride) staining in rice seed viability and nCDA (normalized canonical discriminant analysis) in rice seed vigour were also discussed, where CIELab L* of TTC staining were negatively correlated with vigor index and germination rate, with Pearson’s correlation coefficient of -0.9874, -0.9802 respectively, and CIELab A* of TTC staining were and positively correlated with vigor index and germination rate, with Pearson’s correlation coefficient of 0.9624, 0.9544 respectively, and CIELab A* of nCDA had Pearson’s correlation coefficient of -0.8866 and -0.9340 with vigor index and germination rate, respectively. Besides testing methods, vigour results within and among variety(ies) showed that, there were great variations among the 6 rice varieties, and mean coefficient of variation (CV) of vigor index of individual seed within a variety reached 64.87%, revealing the high risk of conventional methods in random sampling. Vigour variations had close relationship with wavelengths of 780 nm-970 nm, indicating their value in future research.
Advances in alternative splicing identification: deep learning and pantranscriptome
Authors: Shen Fei , Hu Chenyang , Huang Xin , He Hao , Yang Deng , Zhao Jirong , Yang Xiaozeng
Published: September 2023

In plants, alternative splicing is a crucial mechanism for regulating gene expression at the post-transcriptional level, which leads to diverse proteins by generating multiple mature mRNA isoforms and diversify the gene regulation. Due to the complexity and variability of this process, accurate identification of splicing events is a vital step in studying alternative splicing. This article presents the application of alternative splicing algorithms with or without reference genomes in plants, as well as the integration of advanced deep learning techniques for improved detection accuracy. In addition, we also discuss alternative splicing studies in the pan-genomic background and the usefulness of integrated strategies for fully profiling alternative splicing.
Remote sensing continuity: a comparison of HTP platforms and potential challenges with field applications
Authors: Herr Andrew W. , Carter Arron H.
Published: September 2023

In an era of climate change and increased environmental variability, breeders are looking for tools to maintain and increase genetic gain and overall efficiency. In recent years the field of high throughput phenotyping (HTP) has received increased attention as an option to meet this need. There are many platform options in HTP, but ground-based handheld and remote aerial systems are two popular options. While many HTP setups have similar specifications, it is not always clear if data from different systems can be treated interchangeably. In this research, we evaluated two handheld radiometer platforms, Cropscan MSR16R and Spectra Vista Corp (SVC) HR-1024i, as well as a UAS-based system with a Sentera Quad Multispectral Sensor. Each handheld radiometer was used for two years simultaneously with the unoccupied aircraft systems (UAS) in collecting winter wheat breeding trials between 2018-2021. Spectral reflectance indices (SRI) were calculated for each system. SRI heritability and correlation were analyzed in evaluating the platform and SRI usability for breeding applications. Correlations of SRIs were low against UAS SRI and grain yield while using the Cropscan system in 2018 and 2019. Dissimilarly, the SVC system in 2020 and 2021 produced moderate correlations across UAS SRI and grain yield. UAS SRI were consistently more heritable, with broad-sense heritability ranging from 0.58 to 0.80. Data standardization and collection windows are important to consider in ensuring reliable data. Furthermore, practical aspects and best practices for these HTP platforms, relative to applied breeding applications, are highlighted and discussed. The findings of this study can be a framework to build upon when considering the implementation of HTP technology in an applied breeding program.
Insight into regulation of adventitious root formation by arbuscular mycorrhizal fungus and exogenous auxin in tea plant (Camellia sinensis L.) cuttings
Authors: Chen Weili , Shan Wenshu , Niu Tingting , Ye Tao , Sun Qinyu , Zhang Jiaxia
Published: September 2023

Introduction

Adventitious root (AR) development, affected by various biotic and abiotic factors, is the most important procedure in tea plant (Camellia sinensis L.) cutting propagation. Establishing symbiotic relationships with most terrestrial plants, AMF (Arbuscular mycorrhizal fungus) can mediate the AR formation of several herbaceous and woody plants in previous studies.

Methods

In this paper, effects of combined application of AMF and exogenous auxin on AR formation of cuttings from different tea plant varieties (‘Pingyangtezao’, ‘Longjing 43’ and ‘Longjingchangye’) were studied. Then we also performed RNA-Seq analysis with ‘Pingyangtezao’ cuttings aiming to find the possible auxin-related pathway of AM fungal regulation on AR formation. To accurately uncover the regulatory mechanism of AMF on AR formation of tea cuttings, rooting process were separated into four stages (S0, non-rooting; S1, AR protrusion; S2, AR formation and S3, AR elongation) at the same sampling time.

Results and Discussion

Results showed that IBA treatment increased the mycorrhizal colonization rate, especially in ‘Pingyangtezao’ variety (from 37.58% to 46.29%). Both inoculating AMF and addition of IBA promoted the AR formation, and rooting of different tea plant varieties showed different dependence on auxin. AMF could alleviate the effect of auxin-related inhibitors (2,3,5-triiodobenzoic acid, L-α-(Aminooxy)-β-phenylpropionic acid and α-(phenylethyl-2-oxo)-IAA) on rooting of tea cuttings, even though the colonization of AMF was hindered at various degrees. Transcriptomic analysis showed that different numbers of differentially expressed genes (DEGs) at various rooting stages of tea cuttings with the most at S2 stage (1360 DEGs), indicating the increasing regulation by AMF with the development of AR. Similar trend was found in auxin-related DEGs, and family genes of YUC, GH, PIN, LAX, SAUR, AUX, and ABP involved in the AM fungal regulation on AR formation of tea cuttings. Additionally, AMF strongly mediated auxin transport and signal transduction pathways in tea cuttings as showed by the results of correlation analysis. Overall, interaction of AMF and exogenous auxin in promoting rooting and the preliminary mechanism of AMF regulating AR formation of tea cuttings was deciphered in this paper, which may provide a basis for further deep mechanistic research and cutting propagation of tea production.
Differential gene expression provides leads to environmentally regulated soybean seed protein content
Authors: Hooker Julia C. , Smith Myron , Zapata Gerardo , Charette Martin , Luckert Doris , Mohr Ramona M. , Daba Ketema A. , Warkentin Thomas D. , Hadinezhad Mehri , Barlow Brent , Hou Anfu , Lefebvre François , Golshani Ashkan , Cober Elroy R. , Samanfar Bahram
Published: September 2023

Soybean is an important global source of plant-based protein. A persistent trend has been observed over the past two decades that soybeans grown in western Canada have lower seed protein content than soybeans grown in eastern Canada. In this study, 10 soybean genotypes ranging in average seed protein content were grown in an eastern location (control) and three western locations (experimental) in Canada. Seed protein and oil contents were measured for all lines in each location. RNA-sequencing and differential gene expression analysis were used to identify differentially expressed genes that may account for relatively low protein content in western-grown soybeans. Differentially expressed genes were enriched for ontologies and pathways that included amino acid biosynthesis, circadian rhythm, starch metabolism, and lipid biosynthesis. Gene ontology, pathway mapping, and quantitative trait locus (QTL) mapping collectively provide a close inspection of mechanisms influencing nitrogen assimilation and amino acid biosynthesis between soybeans grown in the East and West. It was found that western-grown soybeans had persistent upregulation of asparaginase (an asparagine hydrolase) and persistent downregulation of asparagine synthetase across 30 individual differential expression datasets. This specific difference in asparagine metabolism between growing environments is almost certainly related to the observed differences in seed protein content because of the positive correlation between seed protein content at maturity and free asparagine in the developing seed. These results provided pointed information on seed protein-related genes influenced by environment. This information is valuable for breeding programs and genetic engineering of geographically optimized soybeans.
Vesicle trafficking in rice: too little is known
Authors: Zhu Xiaobo , Yin Junjie , Guo Hongming , Wang Yuping , Ma Bingtian
Published: September 2023

The vesicle trafficking apparatus is a fundamental machinery to maintain the homeostasis of membrane-enclosed organelles in eukaryotic cells. Thus, it is broadly conserved in eukaryotes including plants. Intensive studies in the model organisms have produced a comprehensive picture of vesicle trafficking in yeast and human. However, with respect to the vesicle trafficking of plants including rice, our understanding of the components and their coordinated regulation is very limited. At present, several vesicle trafficking apparatus components and cargo proteins have been identified and characterized in rice, but there still remain large unknowns concerning the organization and function of the rice vesicle trafficking system. In this review, we outline the main vesicle trafficking pathways of rice based on knowledge obtained in model organisms, and summarize current advances of rice vesicle trafficking. We also propose to develop methodologies applicable to rice and even other crops for further exploring the mysteries of vesicle trafficking in plants.
Sustained organic amendments utilization enhances ratoon crop growth and soil quality by enriching beneficial metabolites and suppressing pathogenic bacteria
Authors: Fallah Nyumah , Pang Ziqin , Lin Zhaoli , Nyimbo Witness Joseph , Lin Wenxiong , Mbuya Sylvain Ntambo , Ishimwe Captoline , Zhang Hua
Published: September 2023

Introduction

Organic soil amendments such as filter mud (FM) and biochar (BC) can potentially influence the abundance and composition of metabolites. However, our current understanding of the stimulatory effects of FM and BC’s long-term impact on stress-regulating metabolites, such as abscisic acid (ABA), jasmonic acid (JA), melatonin, and phenyllactic acid (PLA), and these substrates regulatory effects on disease-causing bacteria in sugarcane ratooning field, which is susceptible to nutrients depletion, diseases, etc., remain poorly understood. Additionally, little is known about how the long-term interaction of these substrates and compounds influences sugarcane ratooning soil enzyme activities, nutrient cycling, and crop growth performance.

Methods

To answer these questions, we adopted metabolomics tools combined with high-throughput sequencing to explore the stimulatory effects of the long-term addition of FM and BC on metabolites (e.g., PLA and abscisic aldehyde) and quantify these substrates’ regulatory effects on disease-causing bacteria, soil enzyme activities, nutrient cycling, and crop growth performance.

Results

The result revealed that ratoon crop weight, stem diameter, sugar content, as well as soil physico-chemical properties, including soil nitrate (NH3+-N), organic matter (OM), total nitrogen (TN), total carbon (TC), and β-glucosidase, marked a significant increase under the BC and FM-amended soils. Whereas soil available potassium (AK), NO3–N, cellulase activity, and phosphatase peaked under the BC-amended soil, primarily due to the enduring effects of these substrates and metabolites. Furthermore, BC and FM-amended soils enriched specific stress-regulating metabolites, including JA, melatonin, abscisic aldehyde, etc. The sustained effects of both BC and FM-amended soils suppressed disease-causing bacteria, eventually promoting ratooning soil growth conditions. A number of key bioactive compounds had distinct associations with several beneficial bacteria and soil physico-chemical properties.

Discussion

This study proves that long-term BC and FM application is one of the eco-friendly strategies to promote ratoon crop growth and soil quality through the enrichment of stress-regulating metabolites and the suppression of disease-causing bacteria.
Uniparental expression of ribosomal RNA in ×Festulolium grasses: a link between the genome and nucleolar dominance
Authors: Mahelka Václav , Kopecký David , Majka Joanna , Krak Karol
Published: September 2023

Genome or genomic dominance (GD) is a phenomenon observed in hybrids when one parental genome becomes dominant over the other. It is manifested by the replacement of chromatin of the submissive genome by that of the dominant genome and by biased gene expression. Nucleolar dominance (ND) – the functional expression of only one parental set of ribosomal genes in hybrids – is another example of an intragenomic competitive process which, however, concerns ribosomal DNA only. Although GD and ND are relatively well understood, the nature and extent of their potential interdependence is mostly unknown. Here, we ask whether hybrids showing GD also exhibit ND and, if so, whether the dominant genome is the same. To test this, we used hybrids between Festuca and Lolium grasses (Festulolium), and between two Festuca species in which GD has been observed (with Lolium as the dominant genome in Festulolium and F. pratensis in interspecific Festuca hybrids). Using amplicon sequencing of ITS1 and ITS2 of the 45S ribosomal DNA (rDNA) cluster and molecular cytogenetics, we studied the organization and expression of rDNA in leaf tissue in five hybrid combinations, four generations and 31 genotypes [F. pratensis × L. multiflorum (F1, F2, F3, BC1), L. multiflorum × F. pratensis (F1), L. multiflorum × F. glaucescens (F2), L. perenne × F. pratensis (F1), F. glaucescens × F. pratensis (F1)]. We have found that instant ND occurs in Festulolium, where expression of Lolium-type rDNA reached nearly 100% in all F1 hybrids and was maintained through subsequent generations. Therefore, ND and GD in Festulolium are manifested by the same dominant genome (Lolium). We also confirmed the concordance between GD and ND in an interspecific cross between two Festuca species.
Optimistic narrative future visions: a communication tool for promoting sustainable (plastic) behavior
Authors: Neef Nicolas E. , Fußwinkel Selina , Roos Claudine , Frank Lilli , Shihepo Kapandu , Richter Isabell
Published: September 2023

Plastic pollution is a pressing global issue, necessitating a focus on consumer behavior to curb this problem at its source. To effectively promote sustainable practices, communication strategies that employ future visions have gained attention. This study examines the effects of a narrative video intervention depicting an optimistic future vision concerning single-use plastic bag consumption in South Africa, compared to a representation of the prevailing status quo. Using a preregistered within-subject design, we assess the psychological and emotional responses to two scenarios of which one is illustrating adaptive behaviors toward reduced plastic bag usage, and the other showcasing current consumption patterns. Parametric analyses revealed a shift in emotional states, characterized by a greater experience of positive emotions and a reduced experience of negative emotions following the exposure to the optimistic future scenario video, as compared to the status quo video. Moreover, engagement with the optimistic future scenario was associated with higher levels of perceived behavioral control and behavioral intentions. No significant changes were found regarding sense of responsibility. These findings point to the potential of optimistic future visions to influence individuals at psychological and emotional levels. This renders optimistic future vision communication as an effective tool for sustainable behavior change, particularly in relation to the sustainable use of plastic shopping bags.
A study on the mechanisms of teachers’ academic emotions and motivational beliefs on learning engagement in the context of online training
Authors: Zhang Dongdong , Gao Siyuan , Ren Liling
Published: September 2023

Introduction

In the context of digital transformation of education, online training is one of the important ways for teachers to improve their professionalism and promote the quality of education. However, studies have shown that teachers’ online training suffers from insufficient learning engagement and other problems, so it is crucial to explore the factors influencing teachers’ learning engagement and their mechanisms of action in the context of online training.

Methods

Taking 589 teachers who participated in online training as the research subjects, the study used the methods of survey research and statistical analysis to explore the influence mechanism of teachers’ academic emotions and motivational beliefs on online learning engagement based on the dual perspectives of control value theory and expectancy-value theory.

Results

The study found that: (1) positive-high arousal academic emotions, training self-efficacy, and training task value significantly and positively predicted online learning engagement, respectively; (2) negative-high arousal and negative-low arousal academic emotions significantly and negatively predicted online learning engagement; (3) training self-efficacy and training task value mediated the relationship between positive-high arousal academic emotions, negative-high arousal academic emotions, negative-low arousal academic emotions and online learning engagement, respectively.

Discussion

The study concluded that by creating an immersive learning environment based on the educational meta universe, personalized and precise training based on big data and adaptive technologies, and establishing a multi-dimensional and three-dimensional online learning support service system, which can effectively improve teachers’ online learning engagement and enhance their online training quality and effectiveness.
All it takes is empathy: how virtual reality perspective-taking influences intergroup attitudes and stereotypes
Authors: Chen Vivian Hsueh Hua , Ibasco Gabrielle C.
Published: September 2023

Research in the past decade has demonstrated the potential of virtual reality perspective-taking (VRPT) to reduce bias against salient outgroups. In the perspective-taking literature, both affective and cognitive mechanisms have been theorized and identified as plausible pathways to prejudice reduction. Few studies have systematically compared affective and cognitive mediators, especially in relation to virtual reality, a medium posited to produce visceral, affective experiences. The present study seeks to extend current research on VRPT’s mechanisms by comparing empathy (affective) and situational attributions (cognitive) as dual mediators influencing intergroup attitudes (affective) and stereotypes (cognitive). In a between-subjects experiment, 84 participants were randomly assigned to embody a VR ingroup or outgroup waiting staff at a local food establishment, interacting with an impolite ingroup customer. Results indicated that participants in the outgroup VRPT condition reported significantly more positive attitudes and stereotypes towards outgroup members than those in the ingroup VRPT condition. For both attitudes and stereotypes, empathy significantly mediated the effect of VRPT, but situational attributions did not. Findings from this research provide support for affect as a key component of virtual experiences and how they shape intergroup perceptions. Implications and directions for further research are discussed.
General health literacy, COVID-19-related health literacy, and protective behaviors: evidence from a population-based study in Japan
Authors: Murakami Keiko , Kuriyama Shinichi , Hashimoto Hideki
Published: September 2023

Introduction

Health literacy (HL) can be regarded as a key element of non-pharmaceutical interventions used in emergency responses. The present study aimed to determine the associations of combinations of general HL and COVID-19-related HL with COVID-19 protective behaviors and healthy lifestyle behaviors.

Methods

A questionnaire survey was conducted from December 2020 to January 2021 among residents in Japanese metropolitan areas. Valid responses were received from 1,443 residents. The levels of HL were categorized into four groups: low level in both HLs (reference), high level in general HL only, high level in COVID-19-related HL only, and high level in both HLs. The total scores of eight COVID-19 protective behaviors were dichotomized into low and high adherence. Healthy lifestyle behaviors included healthy and balanced diet, adequate sleep, and regular exercise. Poisson regression analyses were conducted to examine the associations between the HL groups and high adherence to COVID-19 protective behaviors.

Results

High level in COVID-19-related HL only was associated with high adherence to COVID-19 protective behaviors [prevalence ratio (PR), 1.25; 95% confidence interval (CI), 1.09–1.45], while high level in general HL only was associated with healthy and balanced diet (PR, 1.49; 95% CI, 1.04–2.13), adequate sleep (PR, 1.46; 95% CI, 1.02–2.10), and regular exercise (PR, 2.00; 95% CI, 1.29–3.13). High level in both HLs showed the highest prevalence of high adherence to COVID-19 protective behaviors and healthy lifestyle behaviors.

Conclusion

These findings indicate that COVID-19-related HL and general HL can both be considered to enhance protective behaviors.
Testing of worn face mask and saliva for SARS-CoV-2
Authors: Wang Xiaoling , Thwin Ohnmar , Haq Zahin , Dong Zijun , Tisdale Lela , Fuentes Lemuel Rivera , Grobe Nadja , Kotanko Peter
Published: September 2023

Background

Exhaled SARS-CoV-2 can be detected on face masks. We compared tests for SARS-CoV-2 RNA on worn face masks and matched saliva samples.

Methods

We conducted this prospective, observational, case-control study between December 2021 and March 2022. Cases comprised 30 in-center hemodialysis patients with recent COVID-19 diagnosis. Controls comprised 13 hemodialysis patients and 25 clinic staff without COVID-19 during the study period and the past 2 months. Disposable 3-layer masks were collected after being worn for 4 hours together with concurrent saliva samples. ThermoFisher COVID-19 Combo Kit (A47814) was used for RT-PCR testing.

Results

Mask and saliva testing specificities were 99% and 100%, respectively. Test sensitivity was 62% for masks, and 81% for saliva (p = 0.16). Median viral RNA shedding duration was 11 days and longer in immunocompromised versus non-immunocompromised patients (22 vs. 11 days, p = 0.06, log-rank test).

Conclusion

While SARS-CoV-2 testing on worn masks appears to be less sensitive compared to saliva, it may be a preferred screening method for individuals who are mandated to wear masks yet averse to more invasive sampling. However, optimized RNA extraction methods and automated procedures are warranted to increase test sensitivity and scalability. We corroborated longer viral RNA shedding in immunocompromised patients.
Benefits, risks, barriers, and facilitators to cycling: a narrative review
Authors: Logan Greig , Somers Camilla , Baker Graham , Connell Hayley , Gray Stuart , Kelly Paul , McIntosh Emma , Welsh Paul , Gray Cindy M. , Gill Jason M. R.
Published: September 2023

There is large potential to increase cycling participation worldwide. Participation in cycling is associated with lower risk of mortality from any cause, and incidence of cardiovascular disease and type 2 diabetes, as well as positive mental health and well-being. The largest potential for health gains likely to come from increasing participation amongst those who do not currently cycle regularly, rather than encouraging those who already cycle regularly to cycle more. Replacing car journeys with cycling can lead to reductions in air pollution emissions and lower pollutant exposure to the general population. Important gaps and uncertainties in the existing evidence base include: the extent to which the health benefits associated with cycling participation are fully causal due to the observational nature of much of the existing evidence base; the real-world economic cost-benefits of pragmatic interventions to increase cycling participation; and the most effective (combination of) approaches to increase cycling participation. To address these uncertainties, large-scale, long-term randomised controlled trials are needed to: evaluate the effectiveness, and cost-effectiveness, of (combinations of) intervention approaches to induce sustained long-term increases in cycling participation in terms of increases in numbers of people cycling regularly and number of cycling journeys undertaken, across a range of population demographic groups; establish the effects of such interventions on relevant outcomes related to health and wellbeing, economic productivity and wider societal impacts; and provide more robust quantification of potential harms of increasing cycling participation, such as collision risks.
Well-being as a performance pillar: a holistic approach for monitoring tennis players
Authors: Michel Marie-Florine , Girard Olivier , Guillard Vincent , Brechbuhl Cyril
Published: September 2023

This perspective article aims to discuss the usefulness of tools that can assist tennis professionals effectively manage the well-being of their players. This includes identifying and monitoring meaningful metrics (i.e., training load, training intensity, heart rate variability), as well as careful planning of training and competition schedules with appropriate recovery periods. The use of innovative training methods (i.e., repeated-sprint training in hypoxia and heat training), and proper dietary practices, along with biometric assessment for young players, represents should be considered. Adopting a holistic approach to decision-making about training and competition, balancing both health and performance considerations, is crucial for tennis players and their support teams. More research is needed to refine best practices for enhancing tennis performance while prioritizing the well-being of players.
Temporal characterization of biogas slurry: a pre-requisite for sustainable nutrigation in crop production
Authors: Yadav Rashmi , Sudhishri Susama , Khanna Manoj , Lal Khajanchi , Dass Anchal , Kushwaha H. L. , Bandyopadhyay Kalikinkar , Dey Abir , Kushwah Ajay , Nag Ramineni Harsha
Published: September 2023

Biogas slurry serves as a useful organic fertilizer due to its substantial nutrient content, and its characterization enables the evaluation of nutrient content and its efficient utilization. This study focuses on the variations in the nutrient content of biogas slurry from different dairy farm systems located near the ICAR-Indian Agricultural Research Institute (IARI) (New Delhi), Daryapur Kalan (New Delhi), and Madanpur (Uttar Pradesh) during the pre-monsoon, monsoon, and post-monsoon seasons. The study reveals significant variations in macronutrient levels, particularly nitrogen (N), which showed variations exceeding 3% and a wider range of almost 6% during the pre-monsoon and post-monsoon periods. Spatial differences between dairy farms also contributed to the variance, with more than 10% differences observed between IARI and Daryapur Kalan and between IARI and Madanpur. Phosphorus (P) remained stable across seasons with spatial variation, while potassium (K) exhibited a reverse trend. Correlation analysis highlighted strong positive associations between N content and phosphorus (0.959), organic carbon (0.954), pH (0.813), and electrical conductivity (0.806). The findings suggest the use of biogas slurry has a potential to reduce the synthetic fertilizer consumption of N, P, and K by approximately 8.78%, 11.01%, and 14.33%, respectively and using them for further for nutrigation.
Spontaneous rupture of a splenic artery aneurysm causing acute abdomen in a 19-year-old male patient: a case report
Authors: Njoum Yumna , Barqawi Abdallah Deghles , Maree Mohammed
Published: September 2023

Introduction

A splenic artery aneurysm is considered an abnormal dilatation of the splenic artery layers greater than 1 cm in diameter. First described by Beaussier in 1770, it affects 1% of the population but carries a major risk for life-threatening complications of rupture in 3%–10% of cases regardless of its congenital or acquired etiology. The presentation is highly variable, from asymptomatic incidental discovery during routine imaging to aneurysmal rupture causing acute abdomen, massive gastrointestinal bleeding, and hemorrhagic shock.

Case presentation

Herein, we present a 19-year-old male patient who presented with epigastric pain and abdominal rigidity associated with a moderate amount of free peritoneal fluid that was found to be a ruptured SAA after immediate laparoscopy, which was successfully managed with splenectomy.

Conclusion

SAAs are a rare etiology of acute abdomen and hemorrhagic shock but have a very high risk of mortality even upon immediate intervention, requiring a very high level of vigilance and a low threshold for surgical intervention in unstable patients presenting with abdominal pain.
Systems biology platform for efficient development and translation of multitargeted therapeutics
Authors: Azer Karim , Leaf Irina
Published: September 2023

Failure to achieve efficacy is among the top, if not the most common reason for clinical trial failures. While there may be many underlying contributors to these failures, selecting the right mechanistic hypothesis, the right dose, or the right patient population are the main culprits. Systems biology is an inter-disciplinary field at the intersection of biology and mathematics that has the growing potential to increase probability of success in clinical trials, delivering a data-driven matching of the right mechanism to the right patient, at the right dose. Moreover, as part of successful selection of targets for a therapeutic area, systems biology is a prime approach to development of combination therapies to combating complex diseases, where single targets have failed to achieve sufficient efficacy in the clinic. Systems biology approaches have become increasingly powerful with the progress in molecular and computational methods and represent a novel innovative tool to tackle the complex mechanisms of human disease biology, linking it to clinical phenotypes and optimizing multiple steps of drug discovery and development. With increasing ability of probing biology at a cellular and organ level with omics technologies, systems biology is here to stay and is positioned to be one of the key pillars of drug discovery and development, predicting and advancing the best therapies that can be combined together for an optimal pharmacological effect in the clinic. Here we describe a systems biology platform with a stepwise approach that starts with characterization of the key pathways contributing to the Mechanism of Disease (MOD) and is followed by identification, design, optimization, and translation into the clinic of the best therapies that are able to reverse disease-related pathological mechanisms through one or multiple Mechanisms of Action (MOA).
Population Genetic Analysis of a Bread Wheat Panel from Northern and Huang-Huai Agro-Ecological Regions in China
Authors: Jin Xiaojie , Gao Huimin , Liu Qian , Zhao Yun , He Wenchuang , Wang Guijuan , Zhou Yu , Song Zheng , Zhao Xiaobin , Ren Xifeng , Peng Yanchun , Zhang Yingjun
Published: September 2023

Bread wheat (Triticum aestivum L.) is one of the most extensively cultivated cereal crops around the world. Here, we investigated the population structure and genetic diversity of a panel mainly originated from two wheat agro-ecological regions (northern winter wheat region, NW; and the Huang-Huai River Valley’s facultative wheat region, HH) in China based on a 15K SNP array. Population genetic analysis revealed that the optimal population number (K) was three, and the three groups were roughly related to ecological regions, including NW (mainly Hebei), HH1 (Henan-Shaanxi), and HH2 (Shandong). Within HH, HH1 had a higher nucleotide diversity (π = 0.31167), minor allele frequency (MAF = 0.2663), polymorphism information content (PIC = 0.2668), and expected heterozygosity (Hexp = 0.3346) than HH2. Furthermore, our results demonstrated that genetic diversity decreases with the advancement of wheat breeding. Finally, inference of ancestry informative markers indicated that the genomes of the three pure groups from the three provinces (Hebei, Henan, and Shandong) of the two regions have genomic regions with different mosaic patterns derived from the two landrace groups. These findings may facilitate the development of wheat breeding strategies to target novel desired alleles in the future.
Effects of Methyl Jasmonate on Fruit Coloration and Quality Improvement in Pears (Pyrus bretschneideri)
Authors: Li Bo , Zhang Xiangzhan , Han Chunhong , Duan Ruiwei , Yang Jian , Xue Huabai
Published: September 2023

Red-skinned pears with a bright red color and abundant health benefits are favored by consumers. However, fruit coloration and inner quality are usually affected by adverse factors, which lead to a decline in fruit quality and commerciality. Methyl jasmonate (MeJA) has been reported to be involved in many plant processes, including anthocyanin accumulation, while the value of MeJA application for fruit coloration and quality improvement in red-skinned pears is still largely unclear. The application of 0, 0.5, 1.0, or 2.0 mM MeJA at different fruit development stages significantly promoted red coloration in ‘Danxiahong’ pears. Moreover, MeJA treatment increased the fruit soluble solids, improved the total sugar content, decreased the fruit acid content, and significantly increased the total sugar/total acid ratio. However, no significant effect was observed on the fruit’s shape or longitudinal or transverse diameters. RT-qPCR analysis indicated that the expression of anthocyanin biosynthetic regulatory and structural genes, including PbrMYB10, PbrbHLH3, PbrWD40, PbrPAL, PbrCHI, PbrDFR, and other genes, was induced by MeJA treatments. Overall, our findings demonstrate that the application of MeJA plays a significant role in promoting anthocyanin accumulation in pear peels, leading to enhanced fruit coloration. Furthermore, MeJA treatment also positively impacts the improvement of the inner fruit quality. These results not only provide valuable insights into the mechanism of MeJA-mediated coloration but also contribute to a better understanding of the overall role of MeJA in pear fruit development and quality enhancement.
Green Waste from Cucumber (Cucumis sativus L.) Cultivation as a Source of Bioactive Flavonoids with Hypolipidemic Potential
Authors: Olennikov Daniil N. , Kashchenko Nina I.
Published: September 2023

Cucumber is an important agricultural crop that is cultivated under greenhouse conditions. Cucumber cultivation generates substantial quantities of green waste that require proper disposal. The lack of data on the chemical composition of cucumber greens and their biological activity emphasizes the necessity for research on useful products resulting from this widely available waste. Our examination of the flavonoid contents in the leaves and stems of 30 cucumber cultivars revealed 6 cultivars with high flavonoid contents. In addition, the cutting time did not cause negative effects, and we observed a positive effect with 0.05–0.10% organomineral fertilizer application on the flavonoid levels in the greens. Liquid chromatography–mass spectrometry detected 38 apigenin derivatives, including acylated and non-acylated cucumerins and C-, O-, and C,O-glycosides. Among these, 12 known flavonoids and 18 novel compounds were identified. The concentrations of these compounds in the six flavonoid extracts varied at 39.85–181.53 mg/g for the non-acylated flavones, 14.67–293.31 mg/g for the cucumerins, and 401.73–892.17 mg/g for the acylated flavones. Oral administration of the total flavonoid extracts (at a dosage of 100 g/kg/day) resulted in a hypolipidemic effect in hyperlipidemic hamsters, with subsequent normalization of their serum lipid profiles, malondialdehyde levels, and liver antioxidative enzyme activities. These results substantiate the lipid-lowering potential of cucumber waste extracts. Our investigation of the selected flavonoid activity showed that isovitexin-2′′-O-glucoside-6′′-O-p-coumarate (administered at 50 g/kg/day) had the highest hypolipidemic potency. These results can contribute to the practical use of cucumber green waste and the development of novel supplements for diseases linked to high-fat consumption.
Knowledge, Attitudes, and Practices Regarding Antibiotic Sales in Pharmacies in Medellín, Colombia 2023
Authors: Colonia Daniel Ricardo Montes , Ramírez Patiño Daniela , Higuita-Gutiérrez Luis Felipe
Published: September 2023

Objective: Describe the knowledge, attitudes, and practices regarding the sale of antibiotics in pharmacies in Medellín, Colombia. Method: A cross-sectional descriptive study was conducted in 277 selected pharmacies using a stratified sampling method with proportional allocation to represent all areas of the city. Knowledge, attitudes, and practices (KAPs) were assessed using a scale, analyzed with absolute and relative frequencies for each item, and represented in a global score ranging from 0 to 100, with a higher score indicating better KAPs. Data were analyzed using relative frequencies with 95% confidence intervals, the Mann-Whitney U test, the Kruskal-Wallis test, and linear regression. Results: Of the included pharmacies, 52.6% were chain pharmacies, 48.4% were attended by pharmacy assistants, and 59% of pharmacists had more than 5 years of experience. The median knowledge score was 70.8 (IQR 58.3–87.5), with 35.3% of pharmacists believing that antibiotics are effective in treating the common cold, 35.2% for treating COVID-19, and 29.4% considering them available for sale without a medical prescription. The attitude score was 53.3 (40.0–66.7), with 60.9% agreeing that prohibiting the sale of antibiotics without a prescription would decrease their sales. The practice score was 62.5 (40.0–79.2), with 65.4% of pharmacists stating that they sometimes sell antibiotics without a prescription due to patients struggling to obtain a medical consultation, 61.3% admitting to selling antibiotics without a prescription for urinary tract infections, and 41.3% for upper respiratory tract infections. Practices were predominantly influenced by pharmacy type (chain or independent) and, to a lesser extent, by knowledge and attitudes. Conclusion: Pharmacists in Medellín exhibit inadequate knowledge, attitudes, and practices regarding the use and sale of antibiotics without a medical prescription. These findings align with international evidence highlighting the need for educational and regulatory strategies promoting rational antibiotic use in pharmacies.
Research on Students’ Action Behavior Recognition Method Based on Classroom Time-Series Images
Authors: Shou Zhaoyu , Yan Mingbang , Wen Hui , Liu Jinghua , Mo Jianwen , Zhang Huibing
Published: September 2023

Students’ action behavior performance is an important part of classroom teaching evaluation. To detect the action behavior of students in classroom teaching videos, and based on the detection results, the action behavior sequence of individual students in the teaching time of knowledge points is obtained and analyzed. This paper proposes a method for recognizing students’ action behaviors based on classroom time-series images. First, we propose an improved Asynchronous Interaction Aggregation (AIA) network for student action behavior detection. By adding a Multi-scale Temporal Attention (MsTA) module and a Multi-scale Channel Spatial Attention (MsCSA) module to the fast pathway and slow pathway, respectively, the accuracy of student action behavior recognition is improved in SlowFast, which is the backbone network of the improved AIA network,. Second, the Equalized Focal Loss function is introduced to improve the category imbalance that exists in the student action behavior dataset. Experimental results on the student action behavior dataset show that the method proposed in this paper can detect different action behaviors of students in the classroom and has better detection performance compared to the original AIA network. Finally, based on the results of action behavior recognition, the seat number is used as the index to obtain the action behavior sequence of individual students during the teaching time of knowledge points and the performance of students in this period is analyzed.