bluuebunny/crossref_metadata_2025 huggingface dataset:
Kinetic Response of Industrial Flotation Banks: Effect of Particle Size and Mineralogy
Authors: Vallejos Paulina , Yianatos Juan , Rodriguez Marcelo , Cortínez Jorge
Published: May 2023

One of the main problems in current flotation plants and new projects is the significant decrease in feed grades and changes in the mineral composition, which has an important effect on the mineral liberation and associations. Additionally, flotation plants usually operate with a feed throughput higher than the original design, which has a significant impact on the feed particle size. All these conditions affect the metallurgical performance, and they must be evaluated and addressed by improving the control strategies for the optimization of banks. For this purpose, the flotation of valuable minerals along the circuits requires better understanding, considering the compromise between performance and selectivity in flotation circuits. This paper presents a characterization of the different minerals recovered along industrial flotation circuits. Plant sampling campaigns, performed in a copper industrial concentrator in Chile, provided the data for the study. Two rougher flotation banks consisting of cells of 130 m3 and 300 m3, were evaluated. All the samples from the industrial surveys were analysed by screening and mineralogy, and the mineral recovery along the circuits was evaluated per particle size class, liberation class, type of associations, and others. Additionally, operating data on froth depth profiles complemented the evaluation of the mineral composition of concentrate streams along the circuit. The results allowed for identifying and quantifying the main factors that affect the copper recovery and concentrate grade along the flotation banks. We found that minerals with liberation lower than 50% have a significant impact on the recovery. On the other hand, the decrease in the Cu grade in concentrates along the flotation circuits is mainly due to the relative increase of middling minerals (less liberated), non-valuable floatable minerals, such as free pyrite, and gangue entrainment. The characterization of the gangue entrainment flowrate along the flotation banks showed a strong relationship with froth depth, which becomes more critical towards the last cells of the banks.
Sulfide Trace Element Signatures and S- and Pb-Isotope Geochemistry of Porphyry Copper and Epithermal Gold-Base Metal Mineralization in the Elatsite–Chelopech Ore Field (Bulgaria)
Authors: Stefanova Elitsa , Georgiev Stoyan , Peytcheva Irena , Marchev Peter , von Quadt Albrecht , Raicheva Raya , Gerdjikov Ianko , Kouzmanov Kalin , Boyce Adrian , Vennemann Torsten
Published: May 2023

The Elatsite–Chelopech ore field in the northern part of the Panagyurishte district in Central Bulgaria comprises numerous spatially associated porphyry copper and epithermal gold deposits and prospects. In addition to the mineralization and alteration features, trace elements, lead and sulfur isotope signatures of sulfide minerals from porphyry copper, base metal and gold-base metal deposits/prospects have been studied. LA-ICP-MS analyses of pyrite, arsenopyrite and sulfosalt minerals validate them as major carriers for Au, Ag, Sb, Se and Co. Pyrite from the three types of mineralization has specific geochemical characteristics. Pyrite from the porphyry copper deposits/prospects has generally lower total trace element content compared to pyrite from the epithermal prospects, except for Se, Co and Ni. Pyrite from the base metal and gold-base metal veins is enriched in As, Au, Ag, Sb and Pb. In pyrite from the base metal deposits, Co and Ni have contents comparable to the pyrite from the porphyry copper deposits, while pyrite from the gold-base metal veins shows lower Co and Ni. Arsenopyrite from these deposits shows similar features. Similarly, sphalerite from the gold-base metal veins also has lower Co content compared to sphalerite from the base metal veins but higher In and Cu contents. In addition to the close spatial relationships between the Elatsite and Gorna Kamenitsa porphyry Cu deposits and Negarstitsa-West and Dolna Kamenitsa base metal prospects, as well as similarities in the mineralization and alteration styles, the lead isotopic (206Pb/204Pb = 18.61–18.68, 207Pb/204Pb = 15.64–15.65 for porphyry and 206Pb/204Pb = 18.55–18.67, 207Pb/204Pb = 15.64–15.68 for base metal) and sulfur isotopic (δ34S values of −3 to +1‰ for porphyry and δ34S values of −1.7 to +3.5‰ for base metal) signatures of sulfides support the idea of a genetic link between these two types of deposits. The porphyry and base-metal mineralization result from a common major ore-forming event during the Late Cretaceous, corresponding to deep/higher-temperature and shallower/distal/lower-temperature environments, respectively. In particular, more radiogenic lead (206Pb/204Pb = 18.41–18.47, 207Pb/204Pb = 15.67–15.76) and slightly different sulfur isotopic compositions (δ34S values of +3.5 to +10.6‰) of sulfides from the distal gold-base metal veins of Kordunsko Dere, Svishti Plaz and Shipkite might be a consequence of the interaction of the ore-forming fluids with an external older crustal and isotopically positive S source. Alternatively, a different fluid source/event for the formation of these gold-base metal veins may be suggested.
Evaluation of Biological Activity of New 1,2,4-Triazole Derivatives Containing Propionic Acid Moiety
Authors: Paprocka Renata , Wiese-Szadkowska Małgorzata , Kołodziej Przemysław , Kutkowska Jolanta , Balcerowska Sara , Bogucka-Kocka Anna
Published: May 2023

To this day, the quest to find new drugs is still a challenge due to the growing demands of patients suffering from chronic inflammatory diseases and the need for the individualization of therapy. The aim of this research was to synthesize new 1,2,4-triazole derivatives containing propanoic acid moiety and to investigate their anti-inflammatory, antibacterial and anthelmintic activity. Compounds 3a–3g were obtained in reactions of amidrazones 1a–1g with succinic anhydride. Several analyses of proton and carbon nuclear magnetic resonance (1H NMR, 13C NMR, respectively), as well as high-resolution mass spectra (HRMS), confirmed the structures of 1,2,4-triazole derivatives 3a–3g. Toxicity, antiproliferative activity and influence on cytokine release (TNF-α: Tumor Necrosis Factor-α, IL-6: Interleukin-6, IFN-γ: Interferon-γ, and IL-10: Interleukin-10) of the compounds 3a–3g were evaluated in peripheral blood mononuclear cells culture. Moreover, mitogen-stimulated cell culture was used for biological activity tests. The antimicrobial and anthelmintic activity of derivatives 3a–3g were studied against Gram-positive and Gram-negative bacterial strains and Rhabditis sp. culture. Despite the lack of toxicity, compounds 3a–3g significantly reduced the level of TNF-α. Derivatives 3a, 3c and 3e also decreased the release of IFN-γ. Taking all of the results into consideration, compounds 3a, 3c and 3e show the most beneficial anti-inflammatory effects.
Protective Effects of Hemp (Cannabis sativa) Root Extracts against Insulin-Deficient Diabetes Mellitus In Mice
Authors: Kim Yujeong , Kim Wonhee , Kim Soo-Hyun , Sim Kyu-Sang , Kim Ki-Hyun , Cho Kiu-Hyung , Kwon Gi-Seok , Lee Jung-Bok , Kim Jun-Ho
Published: May 2023

The pharmacological potential of industrial hemp (Cannabis sativa) has been widely studied. However, the majority of studies have focused on cannabidiol, isolated from the inflorescence and leaf of the plant. In the present study, we evaluated the anti-diabetic potential of hemp root water (HWE) and ethanol extracts (HEE) in streptozotocin (STZ)-induced insulin-deficient diabetic mice. The administration of HWE and HEE ameliorated hyperglycemia and improved glucose homeostasis and islet function in STZ-treated mice (p < 0.05). HWE and HEE suppressed β-cell apoptosis and cytokine-induced inflammatory signaling in the pancreas (p < 0.05). Moreover, HWE and HEE normalized insulin-signaling defects in skeletal muscles and apoptotic response in the liver and kidney induced by STZ (p < 0.05). Gas chromatography-mass spectrometry analysis of HWE and HEE showed possible active compounds which might be responsible for the observed anti-diabetic potential. These findings indicate the possible mechanisms by which hemp root extracts protect mice against insulin-deficient diabetes, and support the need for further studies geared towards the application of hemp root as a novel bioactive material.
Design, Synthesis, and Biomedical Application of Multifunctional Fluorescent Polymer Nanomaterials
Authors: Bu Qingpan , Li Ping , Xia Yunfei , Hu Die , Li Wenjing , Shi Dongfang , Song Kai
Published: May 2023

Luminescent polymer nanomaterials not only have the characteristics of various types of luminescent functional materials and a wide range of applications, but also have the characteristics of good biocompatibility and easy functionalization of polymer nanomaterials. They are widely used in biomedical fields such as bioimaging, biosensing, and drug delivery. Designing and constructing new controllable synthesis methods for multifunctional fluorescent polymer nanomaterials with good water solubility and excellent biocompatibility is of great significance. Exploring efficient functionalization methods for luminescent materials is still one of the core issues in the design and development of new fluorescent materials. With this in mind, this review first introduces the structures, properties, and synthetic methods regarding fluorescent polymeric nanomaterials. Then, the functionalization strategies of fluorescent polymer nanomaterials are summarized. In addition, the research progress of multifunctional fluorescent polymer nanomaterials for bioimaging is also discussed. Finally, the synthesis, development, and application fields of fluorescent polymeric nanomaterials, as well as the challenges and opportunities of structure–property correlations, are comprehensively summarized and the corresponding perspectives are well illustrated.
Effects of HAT-CN Layer Thickness on Molecular Orientation and Energy-Level Alignment with ZnPc
Authors: Joo Eunah , Hur Jin Woo , Ko Joon Young , Kim Tae Gyun , Hwang Jung Yeon , Smith Kevin E. , Lee Hyunbok , Cho Sang Wan
Published: May 2023

Efficient energy-level alignment is crucial for achieving high performance in organic electronic devices. Because the electronic structure of an organic semiconductor is significantly influenced by its molecular orientation, comprehensively understanding the molecular orientation and electronic structure of the organic layer is essential. In this study, we investigated the interface between a 1,4,5,8,9,11-hexaazatriphenylene hexacarbonitrile (HAT-CN) hole injection layer and a zinc-phthalocyanine (ZnPc) p-type organic semiconductor. To determine the energy-level alignment and molecular orientation, we conducted in situ ultraviolet and X-ray photoelectron spectroscopies, as well as angle-resolved X-ray absorption spectroscopy. We found that the HAT-CN molecules were oriented relatively face-on (40°) in the thin (5 nm) layer, whereas they were oriented relatively edge-on (62°) in the thick (100 nm) layer. By contrast, ZnPc orientation was not significantly altered by the underlying HAT-CN orientation. The highest occupied molecular orbital (HOMO) level of ZnPc was closer to the Fermi level on the 100 nm thick HAT-CN layer than on the 5 nm thick HAT-CN layer because of the higher work function. Consequently, a considerably low energy gap between the lowest unoccupied molecular orbital level of HAT-CN and the HOMO level of ZnPc was formed in the 100 nm thick HAT-CN case. This may improve the hole injection ability of the anode system, which can be utilized in various electronic devices.
Skeleton Synthesis of a Plant-Derived Radioprotective Alkaloid Born to Produce a Novel Fused Heterocycle
Authors: Liu Sifan , Gu Huiling , Liang Kai , Wei Zhenzhen , Li Bin , Tian Ying , Li Ruihong , Zhang Guangjie , Liu Shuchen
Published: May 2023

Alkaloids are a material treasure bestowed on humans by nature owing to their numerous biological activities. Orychophragine D, an alkaloid isolated from the seeds of Orychophragmus violaceus was identified as bearing a novel skeleton and proved to have an excellent radioprotective effect. Different from the common alkaloid structure, the main block of orychophragine D is constructed of an oxotriazine and an oxopiperazine, which are connected in parallel by a C-N bond. In this paper, a preparation method for the novel heterocycle skeleton of orychophragine D is proposed for the first time. N-Boc-L-serine was utilized as the original material to complete the preparation with 11 steps in a 13% overall yield. A hydroxyl group was established on the side chain of the skeleton as the reaction site for researchers to conduct further structural modification or derivatization.
Fine Tuning the Intermolecular Interactions of Water Clusters Using the Dispersion-Corrected Density Functional Theory
Authors: Ferretti Alfonso , Canal Laura , Sorodoc Robert A. , Sinha Sourab , Brancato Giuseppe
Published: May 2023

Dispersion-inclusive density functional theory (DFT) methods have unequivocally demonstrated improved performances with respect to standard DFT approximations for modeling large and extended molecular systems at the quantum mechanical level. Yet, in some cases, disagreements with highly accurate reference calculations, such as CCSD(T) and quantum Monte Carlo (MC) calculations, still remain. Furthermore, the application of general-purpose corrections, such as the popular Grimme’s semi-classical models (DFT-D), to different Kohn–Sham exchange–correlation functionals sometimes leads to variable and inconsistent results, which recommend a careful prior evaluation. In a recent study, we proposed a simple optimization protocol for enhancing the accuracy of these DFT-D methods by following an alternative and system-specific approach. Here, adopting the same computational strategy, we show how the accurate MC intermolecular interactions of a large set of water clusters of variable sizes (i.e., 300 (H2O)n structures, n = 9, 15, 27) can be reproduced remarkably well by dispersion-corrected DFT models (i.e., B3LYP-D4, PBE-D4, revPBE(0)-D4) upon re-optimization, reaching a mean absolute error per monomer of ~0.1 kcal/mol. Hence, the obtained results support the use of this procedure for fine-tuning tailored DFT-D models for the accurate description of targeted molecular systems.
A Facile Hydrothermal Synthesis and Resistive Switching Behavior of α-Fe2O3 Nanowire Arrays
Authors: Yu Zhiqiang , Xu Jiamin , Liu Baosheng , Sun Zijun , Huang Qingnan , Ou Meilian , Wang Qingcheng , Jia Jinhao , Kang Wenbo , Xiao Qingquan , Gao Tinghong , Xie Quan
Published: May 2023

A facile hydrothermal process has been developed to synthesize the α-Fe2O3 nanowire arrays with a preferential growth orientation along the [110] direction. The W/α-Fe2O3/FTO memory device with the nonvolatile resistive switching behavior has been achieved. The resistance ratio (RHRS/RLRS) of the W/α-Fe2O3/FTO memory device exceeds two orders of magnitude, which can be preserved for more than 103s without obvious decline. Furthermore, the carrier transport properties of the W/α-Fe2O3/FTO memory device are dominated by the Ohmic conduction mechanism in the low resistance state and trap-controlled space-charge-limited current conduction mechanism in the high resistance state, respectively. The partial formation and rupture of conducting nanofilaments modified by the intrinsic oxygen vacancies have been suggested to be responsible for the nonvolatile resistive switching behavior of the W/α-Fe2O3/FTO memory device. This work suggests that the as-prepared α-Fe2O3 nanowire-based W/α-Fe2O3/FTO memory device may be a potential candidate for applications in the next-generation nonvolatile memory devices.
Temperature-Sensitive Modified Bentonite Based on NIPAM for Drilling Fluid: Experimental and Molecular Simulation Studies
Authors: Pan Yi , Zhang Xinyue , Zhan Qianru , Yang Shuangchun , Wang Yanchao , Guan Jian , Yang Gang , Yang Peng , Qayum Zain Ullah Abdul
Published: May 2023

Bentonite is an important component of drilling fluid, whose quality directly affects the safety and economic benefits of water-based drilling fluid. In order to effectively cope with temperature changes, the development of temperature-sensitive modified bentonite is of great significance. In this study, a temperature-sensitive modified bentonite based on NIPAM with excellent temperature sensitivity was developed through intercalation modification. The temperature-sensitive bentonite (CMC-B-NIPAM) was prepared by grafting N-isopropyl acrylamide (NIPAM) onto the surface of calcium bentonite through the dehydration condensation of silane coupling agent KH570 after the intercalation of sodium Carboxymethyl Cellulose (CMC). The synthesis indexes of CMC-B and CMC-B-NIPAM were optimized by the single-factor method. CMC-B-NIPAM was characterized by XRD and FTIR. The temperature sensitivity, rheology, suspensibility, and expansion capacity of CMC-B-NIPAM dispersion were investigated. The results showed that CMC-B-NIPAM had good temperature sensitivity, and the rheological properties of its dispersion showed characteristics of steady flow and temperature thickening in the range of 40–70 °C. A molecular simulation model was established to observe the microsynthesis mechanism of temperature-sensitive modified bentonite based on NIPAM. The results of this study show that CMC-B-NIPAM drilling fluid has the function of ensuring the stability of drilling fluid flow patterns compared to traditional drilling fluids.
NMR-Based Metabolic Profiling of the Effects of α-Ketoglutarate Supplementation on Energy-Deficient C2C12 Myotubes
Authors: Li Yantong , Zhang Shuya , Huang Caihua , Lin Donghai
Published: May 2023

Skeletal muscle is closely linked to energy metabolism, but it is inevitably deprived of energy. Cellular differentiation is an essential and energy-demanding process in skeletal muscle development. Much attention has been paid to identifying beneficial factors that promote skeletal muscle satellite cell differentiation and further understanding the underlying regulatory mechanisms. As a critical metabolic substrate or regulator, α-ketoglutarate (AKG) has been recognized as a potential nutritional supplement or therapeutic target for skeletal muscle. We have previously found beneficial effects of AKG supplementation on the proliferation of C2C12 myoblasts cultured under both normal and energy-deficient conditions and have further elucidated the underlying metabolic mechanisms. However, it remains unclear what role AKG plays in myotube formation in different energy states. In the present study, we investigated the effects of AKG supplementation on the differentiation of C2C12 myoblasts cultured in normal medium (Nor myotubes) and low glucose medium (Low myotubes) and performed NMR-based metabonomic profiling to address AKG-induced metabolic changes in both Nor and Low myotubes. Significantly, AKG supplementation promoted myotube formation and induced metabolic remodeling in myotubes under normal medium and low glucose medium, including improved energy metabolism and enhanced antioxidant capacity. Specifically, AKG mainly altered amino acid metabolism and antioxidant metabolism and upregulated glycine levels and antioxidase expression. Our results are typical for the mechanistic understanding of the effects of AKG supplementation on myotube formation in the two energy states. This study may be beneficial for further exploring the applications of AKG supplementation in sports, exercise, and therapy.
Synthesis and Photocatalytic Properties of Four Coordination Compounds Constructed from Two Benzimidazole-Based Asymmetric Polyazocyclic Ligands
Authors: Ren Chenfei , Li Jian , Zhang Xingxing , Niu Yunyin
Published: May 2023

In this paper, two benzimidazole derivative ligands were obtained using o-phenylenediamine and n-pyridine formaldehyde (n = 3, 4) by amine–aldol condensation reactions, which were reacted with selected inorganic metal salts by ambient temperature volatilization method to give compounds 1–4: {[(L1)6]·[Cu8I8]} (1), {[L1]·[CuBr]·H2O} (2), {[L2]·[CuBr]}n (3), and {[(L2)4]·[Cu4I4]} (4). They were characterized by IR, UV-Vis absorption spectroscopy, thermogravimetric analysis, and single crystal X-ray analysis. Simultaneously, compounds 1–4 were found to possess photocatalytic degradation of ciprofloxacin (CIP) by preliminary experimental investigations.
Herpetin Promotes Bone Marrow Mesenchymal Stem Cells to Alleviate Carbon Tetrachloride-Induced Acute Liver Injury in Mice
Authors: Ding Yi , Tan Rui , Gu Jian , Gong Puyang
Published: May 2023

Herpetin, an active compound derived from the seeds of Herpetospermum caudigerum Wall., is a traditional Tibetan herbal medicine that is used for the treatment of hepatobiliary diseases. The aim of this study was to evaluate the stimulant effect of herpetin on bone marrow mesenchymal stem cells (BMSCs) to improve acute liver injury (ALI). In vitro results showed that herpetin treatment enhanced expression of the liver-specific proteins alpha-fetoprotein, albumin, and cytokeratin 18; increased cytochrome P450 family 3 subfamily a member 4 activity; and increased the glycogen-storage capacity of BMSCs. Mice with ALI induced by carbon tetrachloride (CCl4) were treated with a combination of BMSCs by tail-vein injection and herpetin by intraperitoneal injection. Hematoxylin and eosin staining and serum biochemical index detection showed that the liver function of ALI mice improved after administration of herpetin combined with BMSCs. Western blotting results suggested that the stromal cell-derived factor-1/C-X-C motif chemokine receptor 4 axis and the Wnt/β-catenin pathway in the liver tissue were activated after treatment with herpetin and BMSCs. Therefore, herpetin is a promising BMSC induction agent, and coadministration of herpetin and BMSCs may affect the treatment of ALI.
L-Theanine and Immunity: A Review
Authors: Chen Shuna , Kang Jiaxin , Zhu Huanqing , Wang Kaixi , Han Ziyi , Wang Leyu , Liu Junsheng , Wu Yuanyuan , He Puming , Tu Youying , Li Bo
Published: May 2023

L-theanine (N-ethyl-γ-glutamine) is the main amino acid in tea leaves. It not only contributes to tea flavor but also possesses several health benefits. Compared with its sedative and calming activities, the immunomodulatory effects of L-theanine have received less attention. Clinical and epidemiological studies have shown that L-theanine reduces immunosuppression caused by strenuous exercise and prevents colds and influenza by improving immunity. Numerous cell and animal studies have proven that theanine plays an immunoregulatory role in inflammation, nerve damage, the intestinal tract, and tumors by regulating γδT lymphocyte function, glutathione (GSH) synthesis, and the secretion of cytokines and neurotransmitters. In addition, theanine can be used as an immunomodulator in animal production. This article reviews the research progress of L-theanine on immunoregulation and related mechanisms, as well as its application in poultry and animal husbandry. It is hoped that this work will be beneficial to future related research.
Supercritical Carbon Dioxide Technology for Recovering Valuable Phytochemicals from Cannabis sativa L. and Valorization of Its Biomass for Food Applications
Authors: de Aguiar Ana Carolina , Vardanega Renata , Viganó Juliane , Silva Eric Keven
Published: May 2023

Supercritical carbon dioxide (CO2) extraction techniques meet all-new consumer market demands for health-promoting phytochemical compound-rich extracts produced from green and sustainable technology. In this regard, this review is dedicated to discussing is the promise of integrating high-pressure CO2 technologies into the Cannabis sativa L. processing chain to valorize its valuable pharmaceutical properties and food biomass. To do this, the cannabis plant, cannabinoids, and endocannabinoid system were reviewed to understand their therapeutic and side effects. The supercritical fluid extraction (SFE) technique was presented as a smart alternative to producing cannabis bioproducts. The impact of SFE operating conditions on cannabis compound extraction was examined for aerial parts (inflorescences, stems, and leaves), seeds, and byproducts. Furthermore, the opportunities of using non-thermal supercritical CO2 processing on cannabis biomass were addressed for industrial hemp valorization, focusing on its biorefinery to simultaneously produce cannabidiol and new ingredients for food applications as plant-based products.
High Thermoelectric Performance of a Novel γ-PbSnX2 (X = S, Se, Te) Monolayer: Predicted Using First Principles
Authors: Ding Changhao , Duan Zhifu , Luo Nannan , Zeng Jiang , Ren Wei , Tang Liming , Chen Keqiu
Published: May 2023

Two-dimensional (2D) group IV metal chalcogenides are potential candidates for thermoelectric (TE) applications due to their unique structural properties. In this paper, we predicted a 2D monolayer group IV metal chalcogenide semiconductor γ-PbSn2 (X = S, Se, Te), and first-principles calculations and Boltzmann transport theory were used to study the thermoelectric performance. We found that γ-PbSnX2 had an ultra-high carrier mobility of up to 4.04 × 103 cm2 V−1 s−1, which produced metal-like electrical conductivity. Moreover, γ-PbSn2 not only has a very high Seebeck coefficient, which leads to a high power factor, but also shows an intrinsically low lattice thermal conductivity of 6–8 W/mK at room temperature. The lower lattice thermal conductivity and high power factors resulted in excellent thermoelectric performance. The ZT values of γ-PbSnS2 and γ-PbSnSe2 were as high as 2.65 and 2.96 at 900 K, respectively. The result suggests that the γ-PbSnX2 monolayer is a better candidates for excellent thermoelectric performance.
Carbon Nanotube-Supported Dummy Template Molecularly Imprinted Polymers for Selective Adsorption of Amide Herbicides in Aquatic Products
Authors: Zeng Sili , Li Chenhui , Huang Li , Chen Zhongxiang , Wang Peng , Qin Dongli , Gao Lei
Published: May 2023

In this study, a carbon nanotube (CNTs)—supported dummy template molecularly imprinted polymer (DMIPs) material was synthesized and utilized for the detection of amide herbicides in aquatic products via matrix solid-phase dispersion technology (MSPD). The DMIPs material was characterized, and its adsorption kinetics and isotherm were determined, the adsorption model was established, and the selective adsorption coefficient was calculated. The extract parameters of the method were optimized and successfully employed for the separation, analysis and detection of real samples, with satisfactory detection limits and linear ranges obtained. By comparing with other methods, the CNTs@DMIPs combined with MSPD technology established in our study can effectively solve false negative problems caused by insufficient destructive force, using dummy template molecules can also address the issue of false positives caused by template molecule leakage in molecular imprinting. Overall, the method is appropriate for the separation and detection of endogenous substances from highly viscous and poorly dispersed samples and is used as a routine detection tool in the aquaculture industry.
Lead-Free FACsSnI3 Based Perovskite Solar Cell: Designing Hole and Electron Transport Layer
Authors: Moiz Syed Abdul , Alahmadi Ahmed N. M. , Alshaikh Mohammed Saleh
Published: May 2023

In recent years, lead-based perovskites solar cells have demonstrated excellent power-conversion efficiency. Despite their remarkable progress, the commercialization of lead-based perovskites is hampered by lead toxicity concerns. The recently discovered non-toxic FACsSnI3 perovskite has the potential to replace lead-based perovskites in solar cell applications. Since the perovskite material FACsSnI3 (FA0.85Cs0.15SnI3) is relatively new, there is a lack of information, particularly regarding the design features required for electron and hole-transport layers for efficient photovoltaic responses. The important variables, such as electron affinity, energy band gap, film thickness, and doping density of both electron and hole-transport layers, were simulated and modeled separately and iteratively in this study to achieve the most efficient photovoltaic response. Finally, the absorber layer thickness of FACsSnI3 perovskite is tuned to achieve a maximum power-conversion efficiency of slightly more than 24%. We hope that the findings of this study will serve as a strong guideline for future research and the design of lead-free perovskite solar cells for efficient photovoltaic responses.
Synthesis and Characterization of Gold Chiral Nanoparticles Functionalized by a Chiral Drug
Authors: Bettini Simona , Ottolini Michela , Valli Donato , Pagano Rosanna , Ingrosso Chiara , Roeffaers Maarten , Hofkens Johan , Valli Ludovico , Giancane Gabriele
Published: May 2023

Inorganic chiral nanoparticles are attracting more and more attention due to their peculiar optical properties and potential biological applications, such as bioimaging, therapeutics, and diagnostics. Among inorganic chiral nanoparticles, gold chiral nanostructures were demonstrated to be very interesting in this context, with good physical chemical stability and also the possibility to decorate the surface, improving biomedical application as the interaction with the bio-systems. Gold (Au) nanostructures were synthesized according to a seed-mediated procedure which envisages the use of cetyltrimethylammonium bromide (CTAB) as the capping agent and L- and D-cysteine to promote chirality. Au nanostructures have been demonstrated to have opposite circular dichroism signals depending on the amino acid enantiomer used during the synthesis. Then, a procedure to decorate the Au surface with penicillamine, a drug used for the treatment of Wilson’s disease, was developed. The composite material of gold nanoparticles/penicillamine was characterized using electron microscopy, and the penicillamine functionalization was monitored by means of UV-Visible, Raman, and infrared spectroscopy, highlighting the formation of the Au–S bond. Furthermore, electron circular dichroism was used to monitor the chirality of the synthesized nanostructures and it was demonstrated that both penicillamine enantiomers can be successfully bonded with both the enantiomers of the gold nanostructures without affecting gold nanoparticles’ chirality. The effective modification of nanostructures’ surfaces via penicillamine introduction allowed us to address the important issue of controlling chirality and surface properties in the chiral nano-system.
Microspheres with 2D rGO/Alginate Matrix for Unusual Prolonged Release of Cefotaxime
Authors: Gomaa Islam , Emam Merna H. , Wassel Ahmed R. , Ashraf Kholoud , Hussan Sara , Kalil Haitham , Bayachou Mekki , Ibrahim Medhat A.
Published: May 2023

A synergistic interaction between reduced graphene oxide (rGO) and a biodegradable natural polymer, sodium alginate, was developed to create unique microspheres with protruding spiky features at the surface (spiky microspheres) that act as a super encapsulation and sustained release system for the highly effective antibiotic cefotaxime. Three forms of microspheres, namely alginate (Alg), alginate-cefotaxime (Alg-CTX), and alginate-cefotaxime-reduced graphene (Alg-CTX-rGO) composites, were prepared using calcium chloride as a cross-linking agent. The microspheres were characterized using field emission scanning electron microscopy (FESEM), Fourier-transform infrared (FT-IR) spectroscopy, and X-ray diffraction to investigate their pores, roughness, surface morphology, functional groups, phase formation, purity, and structural properties. The membrane diffusion method was employed to determine the release profile of Cefotaxime from the fabricated microspheres. The antibacterial activities of CTX solution, Alg microspheres, Alg-CTX microspheres, and Alg-CTX-rGO microspheres were investigated against gram-negative bacteria (Escherichia coli) using the agar diffusion method on Muller–Hinton agar. The prepared samples exhibited excellent results, suggesting their potential for enhanced antibiotic delivery. The results demonstrated the potential of the microsphere 2D rGO/alginate matrix for enhancing cefotaxime delivery with an unusual, prolonged release profile.
BiVO4 As a Sustainable and Emerging Photocatalyst: Synthesis Methodologies, Engineering Properties, and Its Volatile Organic Compounds Degradation Efficiency
Authors: Kamble Ganesh S. , Natarajan Thillai Sivakumar , Patil Santosh S. , Thomas Molly , Chougale Rajvardhan K. , Sanadi Prashant D. , Siddharth Umesh S. , Ling Yong-Chein
Published: May 2023

Bismuth vanadate (BiVO4) is one of the best bismuth-based semiconducting materials because of its narrow band gap energy, good visible light absorption, unique physical and chemical characteristics, and non-toxic nature. In addition, BiVO4 with different morphologies has been synthesized and exhibited excellent visible light photocatalytic efficiency in the degradation of various organic pollutants, including volatile organic compounds (VOCs). Nevertheless, the commercial scale utilization of BiVO4 is significantly limited because of the poor separation (faster recombination rate) and transport ability of photogenerated electron–hole pairs. So, engineering/modifications of BiVO4 materials are performed to enhance their structural, electronic, and morphological properties. Thus, this review article aims to provide a critical overview of advanced oxidation processes (AOPs), various semiconducting nanomaterials, BiVO4 synthesis methodologies, engineering of BiVO4 properties through making binary and ternary nanocomposites, and coupling with metals/non-metals and metal nanoparticles and the development of Z-scheme type nanocomposites, etc., and their visible light photocatalytic efficiency in VOCs degradation. In addition, future challenges and the way forward for improving the commercial-scale application of BiVO4-based semiconducting nanomaterials are also discussed. Thus, we hope that this review is a valuable resource for designing BiVO4-based nanocomposites with superior visible-light-driven photocatalytic efficiency in VOCs degradation.
Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis
Authors: Alves Alex Barbosa , da Silva Bortoleti Bruna Taciane , Tomiotto-Pellissier Fernanda , Ganaza Ana Flávia Marques , Gonçalves Manoela Daiele , Carloto Amanda Cristina Machado , Rodrigues Ana Carolina Jacob , Silva Taylon Felipe , Nakazato Gerson , Kobayashi Renata Katsuko Takayama , Lazarin-Bidóia Danielle , Miranda-Sapla Milena Menegazzo , Costa Idessania Nazareth , Pavanelli Wander Rogério , Conchon-Costa Ivete
Published: May 2023

American tegumentary leishmaniasis, a zoonotic disease caused by the Leishmania genus, poses significant challenges in treatment, including administration difficulty, low efficacy, and parasite resistance. Novel compounds or associations offer alternative therapies, and natural products such as oregano essential oil (OEO), extracted from Origanum vulgare, have been extensively researched due to biological effects, including antibacterial, antifungal, and antiparasitic properties. Silver nanoparticles (AgNp), a nanomaterial with compelling antimicrobial and antiparasitic activity, have been shown to exhibit potent leishmanicidal properties. We evaluated the in vitro effect of OEO and AgNp-Bio association on L. amazonensis and the death mechanisms of the parasite involved. Our results demonstrated a synergistic antileishmanial effect of OEO + AgNp on promastigote forms and L. amazonensis-infected macrophages, which induced morphological and ultrastructural changes in promastigotes. Subsequently, we investigated the mechanisms underlying parasite death and showed an increase in NO, ROS, mitochondrial depolarization, accumulation of lipid-storage bodies, autophagic vacuoles, phosphatidylserine exposure, and damage to the plasma membrane. Moreover, the association resulted in a reduction in the percentage of infected cells and the number of amastigotes per macrophage. In conclusion, our findings establish that OEO + AgNp elicits a late apoptosis-like mechanism to combat promastigote forms and promotes ROS and NO production in infected macrophages to target intracellular amastigote forms.
Clinical Spectrum and Outcomes of Cryptogenic Klebsiella pneumoniae Liver Abscess in the Americas: A Scoping Review
Authors: Cardenas-Alvarez Jorge , Balayla Galit , Triana Abel , Diaz Lankenau Rodrigo , Franco-Paredes Carlos , Henao-Martínez Andrés F. , Motoa Gabriel
Published: May 2023

(1) Background: Cryptogenic Klebsiella pneumoniae liver abscesses are an invasive infection with or without extra hepatic involvement in the absence of hepatobiliary disease or abdominal malignancy. Most of the evidence has emanated from reports from Asia, and previous studies in the Americas have limited clinical characterization. (2) Methods: To understand this syndrome’s characteristics on our continent, we conducted a scoping review to identify adult cases of idiopathic, community-acquired monomicrobial K. pneumoniae liver abscess in the Americas. (3) Results: We identified 144 cases spanning 1978–2022. Most cases were reported in males that had traveled or migrated from Southeast or East Asia with diabetes mellitus. Extrahepatic involvement and bacteremia were common, including seeding to the lungs, ocular structures, and central nervous system. Although limited by sample size, the most commonly reported genes were magA or rmpA. Concomitant percutaneous drainage and third generation cephalosporins (alone or in combination with other antibiotics) were frequently used, yet pooled fatality occurred in 9% of the reported cases. (4) Conclusions: The features of cryptogenic K. pneumoniae liver abscess in the Americas mirror those described in Asia, confirming its global dissemination. This condition is increasingly being reported in our continent and carries significant clinical impact due to its systemic invasiveness.
New Insights on the Neglected Avian Nematode Hystrichis tricolor: Hystrichiosis-Induced Proventriculitis in Synanthropic Egyptian Geese (Alopochen aegyptiaca Linnaeus, 1766) in Germany
Authors: Fischer Ella F. , Schlohsarczyk Elfi K. , Gröf Manuela , Gärtner Ulrich , Taubert Anja , Hermosilla Carlos
Published: May 2023

Hystrichis tricolor is a neglected avian enoplid nematode (superfamiliy Dioctophymatoidea) and known to parasitize various species of the Anatidae (Anas spp. and Mergus spp.) from the northern hemisphere, inducing mainly proventriculitis in domestic and wild waterfowl. Here, we focus on the pathological findings of naturally H. tricholor-infected Egyptian geese (Alopochen aegyptiaca) and a neozoan shelduck (Tandorninae) of Germany. Nowadays, this species is considered the fastest-spreading alien waterfowl species within Western Europe. Additionally, molecular sequencing coupled with phylogenetic characterization of H. tricolor is reported. Post mortem analyses unveiled patent gastric H. tricolor infections in eight of twelve infected birds (8/12; 66.7%), inducing proventriculitis resulting in large visible nodular lesions. Histopathological findings point to chronic host pro-inflammatory immune reactions. These results demonstrate the potential role of Egyptian geese as natural reservoir hosts of H. tricholor and highlight their possible role in parasite transmission (i.e., spillback) into endemic waterfowl species. Due to avian health concerns, the occurrence of hystrichiosis should be monitored in native waterfowl in the future, introducing appropriate management practices in conservation strategies of endemic wild birds not only in Germany but elsewhere in Europe.
Inflammatory and Immune Responses during SARS-CoV-2 Infection in Vaccinated and Non-Vaccinated Pregnant Women and Their Newborns
Authors: Zelini Paola , d’Angelo Piera , Zavaglio Federica , Soleymaninejadian Ehsan , Mariani Liliana , Perotti Francesca , Dominoni Mattia , Tonello Stelvio , Sainaghi Pierpaolo , Minisini Rosalba , Apostolo Daria , Lilleri Daniele , Spinillo Arsenio , Baldanti Fausto
Published: May 2023

Background. Pregnant women are more susceptible to severe disease associated with SARS-CoV-2 infection. We performed a prospective study to analyze the inflammatory and immune profile after SARS-CoV-2 infection occurring in vaccinated or non-vaccinated pregnant women and their newborns. Methods. Twenty-five pregnant women with SARS-CoV-2 infection were enrolled, and sixteen cord blood samples were obtained at delivery. Results. We observed that IL-1β, TNF-α, Eotaxin, MIB-1β, VEGF, IL-15, IL-2, IL-5, IL-9, IL-10 and IL-1ra levels were significantly higher in vaccinated than non-vaccinated mothers. Furthermore, the newborns of the vaccinated mothers produced higher levels of IL-7, IL-5 and IL-12 compared to the newborns of non-vaccinated mothers. Anti-Spike (S) IgG levels were significantly higher in all vaccinated mothers and their newborns compared to the non-vaccinated group. We found that 87.5% of vaccinated women and 66.6% of non-vaccinated women mounted an S-specific T-cell response quantified by ELISpot assay. Moreover, 75.0% of vaccinated mothers and 38.4% of non-vaccinated mothers showed S-specific CD4+ T-cell proliferative response. The T-helper subset response was restricted to CD4+ Th1 in both vaccinated and non-vaccinated women. Conclusion. A higher level of cytokines, IgG antibodies and memory T cells was noted in the vaccinated women. Furthermore, the maternal IgG antibody trans-placental transfer occurred more frequently in vaccinated mothers and may protect the newborn.
Reduced Type 2 Innate Lymphocyte Cell Frequencies in Patent Wuchereria bancrofti-Infected Individuals
Authors: Tamadaho Ruth S. E. , Osei-Mensah Jubin , Arndts Kathrin , Debrah Linda Batsa , Debrah Alexander Y. , Layland Laura E. , Hoerauf Achim , Pfarr Kenneth , Ritter Manuel
Published: May 2023

Approximately 51 million individuals suffer from lymphatic filariasis (LF) caused mainly by the filarial worm Wuchereria bancrofti. Mass drug administration (MDA) programs led to a significant reduction in the number of infected individuals, but the consequences of the treatment and clearance of infection in regard to host immunity remain uncertain. Thus, this study investigates the composition of myeloid-derived suppressor cells (MDSCs), macrophage subsets and innate lymphoid cells (ILCs), in patent (circulating filarial antigen (CFA)+ microfilariae (MF)+) and latent (CFA+MF−) W. bancrofti-infected individuals, previously W. bancrofti-infected (PI) individuals cured of the infection due to MDA, uninfected controls (endemic normal (EN)) and individuals who suffer from lymphoedema (LE) from the Western Region of Ghana. Frequencies of ILC2 were significantly reduced in W. bancrofti-infected individuals, while the frequencies of MDSCs, M2 macrophages, ILC1 and ILC3 were comparable between the cohorts. Importantly, clearance of infection due to MDA restored the ILC2 frequencies, suggesting that ILC2 subsets might migrate to the site of infection within the lymphatic tissue. In general, the immune cell composition in individuals who cured the infection were comparable to the uninfected individuals, showing that filarial-driven changes of the immune responses require an active infection and are not maintained upon the clearance of the infection.
Infection with Seasonal H1N1 Influenza Results in Comparable Disease Kinetics and Host Immune Responses in Ferrets and Golden Syrian Hamsters
Authors: Paterson Jemma , Ryan Kathryn A. , Morley Daniel , Jones Nicola J. , Yeates Paul , Hall Yper , Whittaker Catherine J. , Salguero Francisco J. , Marriott Anthony C.
Published: May 2023

Animal models of influenza are important in preclinical research for the study of influenza infection and the assessment of vaccines, drugs and therapeutics. Here, we show that Golden Syrian hamsters (Mesocricetus auratus) inoculated via the intranasal route with high dose of influenza H1N1 display comparable disease kinetics and immune responses to the ‘gold standard’ ferret (Mustela furo) model. We demonstrate that both the hamster and ferret models have measurable disease endpoints of weight loss, temperature change, viral shedding from the upper respiratory tract and increased lung pathology. We also characterised both the humoral and cellular immune responses to infection in both models. The comparability of these data supports the Golden Syrian hamster model being useful in preclinical evaluation studies to explore the efficacy of countermeasures against influenza.
Valley-Selective Polarization in Twisted Bilayer Graphene Controlled by a Counter-Rotating Bicircular Laser Field
Authors: Chen Jiayin , Liu Candong , Li Ruxin
Published: May 2023

The electron valley pseudospin in two-dimensional hexagonal materials is a crucial degree of freedom for achieving their potential application in valleytronic devices. Here, bringing valleytronics to layered van der Waals materials, we theoretically investigate lightwave-controlled valley-selective excitation in twisted bilayer graphene (tBLG) with a large twist angle. It is demonstrated that the counter-rotating bicircular light field, consisting of a fundamental circularly-polarized pulse and its counter-rotating second harmonic, can manipulate the sub-cycle valley transport dynamics by controlling the relative phase between two colors. In comparison with monolayer graphene, the unique interlayer coupling of tBLG renders its valley selectivity highly sensitive to duration, leading to a noticeable valley asymmetry that is excited by single-cycle pulses. We also describe the distinct signatures of the valley pseudospin change in terms of observing the valley-selective circularly-polarized high-harmonic generation. The results show that the valley pseudospin dynamics can still leave visible fingerprints in the modulation of harmonic signals with a two-color relative phase. This work could assist experimental researchers in selecting the appropriate protocols and parameters to obtain ideal control and characterization of valley polarization in tBLG.
Predicting Maize Theoretical Methane Yield in Combination with Ground and UAV Remote Data Using Machine Learning
Authors: Kavaliauskas Ardas , Žydelis Renaldas , Castaldi Fabio , Auškalnienė Ona , Povilaitis Virmantas
Published: May 2023

The accurate, timely, and non-destructive estimation of maize total-above ground biomass (TAB) and theoretical biochemical methane potential (TBMP) under different phenological stages is a substantial part of agricultural remote sensing. The assimilation of UAV and machine learning (ML) data may be successfully applied in predicting maize TAB and TBMP; however, in the Nordic-Baltic region, these technologies are not fully exploited. Therefore, in this study, during the maize growing period, we tracked unmanned aerial vehicle (UAV) based multispectral bands (blue, red, green, red edge, and infrared) at the main phenological stages. In the next step, we calculated UAV-based vegetation indices, which were combined with field measurements and different ML models, including generalized linear, random forest, as well as support vector machines. The results showed that the best ML predictions were obtained during the maize blister (R2)–Dough (R4) growth period when the prediction models managed to explain 88–95% of TAB and 88–97% TBMP variation. However, for the practical usage of farmers, the earliest suitable timing for adequate TAB and TBMP prediction in the Nordic-Baltic area is stage V7–V10. We conclude that UAV techniques in combination with ML models were successfully applied for maize TAB and TBMP estimation, but similar research should be continued for further improvements.