bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigation of Cytotoxicity and Cell Uptake of Cationic Beta-Cyclodextrins as Valid Tools in Nasal Delivery
Authors: Rassu Giovanna , Fancello Silvia , Roldo Marta , Malanga Milo , Szente Lajos , Migheli Rossana , Gavini Elisabetta , Giunchedi Paolo
Published: July 2020

Cyclodextrin polymers have high applicability in pharmaceutical formulations due to better biocompatibility, solubility enhancement, loading capacity and controlled drug release than parent the cyclodextrins. The cytotoxicity and cell uptake of new cationic beta-cyclodextrin monomers and polymers were evaluated as suitable material for nasal formulations and their protective effects on cells exposed to hydrogen peroxide were studied. PC12 and CACO-2 cells were selected as the neuronal and epithelial type cells, respectively, to mimic the structure of respiratory and olfactory epithelia of the nasal cavity. All cationic beta-cyclodextrin polymers tested showed dose- and time-dependent toxicity; nevertheless, at 5 µM concentration and 60 min of exposure, the quaternary-ammonium-beta-cyclodextrin soluble polymer could be recognized as non-toxic. Based on these results, fluorescently labelled quaternary-ammonium-beta-cyclodextrin monomer and polymer were selected for uptake studies in CACO-2 cells. The monomeric and polymeric beta-cyclodextrins were internalized in the cytoplasm of CACO-2 cells; the cationic monomer showed higher permeability than the hydroxypropyl-beta-cyclodextrin, employed as comparison. Therefore, these cationic beta-cyclodextrins showed potential as excipients able to improve the nasal absorption of drugs. Furthermore, amino-beta-cyclodextrin and beta-cyclodextrin soluble polymers were able to reduce oxidative damage in PC12 and CACO-2 cells and thus could be studied as bioactive carriers or potential drugs for cells protection against oxidative stress.
Biomimetic Catalysts Based on Au@ZnO-Graphene Composites for the Generation of Hydrogen by Water Splitting
Authors: Machín Abniel , Arango Juan C , Fontánez Kenneth , Cotto María , Duconge José , Soto-Vázquez Loraine , Resto Edgar , Petrescu Florian Ion Tiberiu , Morant Carmen , Márquez Francisco M.
Published: July 2020

For some decades, the scientific community has been looking for alternatives to the use of fossil fuels that allow the planet's sustainable and environmentally friendly development. To do this, attempts have been made to mimic some processes that occur in nature, among which the photosystem-II stands out, which allows water splitting operating with different steps to generate oxygen and hydrogen. This research presents promising results using synthetic catalysts, which try to simulate some natural processes, and which are based on Au@ZnO-graphene compounds. These catalysts were prepared by incorporating different amounts of gold nanoparticles (1 wt.%, 3 wt.%, 5 wt.%, 10 wt.%) and graphene (1 wt.%) on the surface of synthesized zinc oxide nanowires (ZnO NWs), and zinc oxide nanoparticles (ZnO NPs), along with a commercial form (commercial ZnO) for comparison purposes. The highest amount of hydrogen (1,127 µmol/hg) was reported by ZnO NWs with a gold and graphene loadings of 10 wt.% and 1 wt.%, respectively, under irradiation at 400 nm. Quantities of 759 µmol / hg and 709 µmol / hg were obtained with catalysts based on ZnO NPs and commercial ZnO, respectively. The photocatalytic activity of all composites increased with respect to the bare semiconductors, being 2.5 times higher in ZnO NWs, 8.8 times for ZnO NPs and 7.5 times for commercial ZnO. The high photocatalytic activity of the catalysts is attributed, mainly, to the synergism between the different amount of gold and graphene incorporated, and the surface area of the composites.
HnRNPA1 and Its Effect in the Expression of ABCC (ABCC4 and ABCC6) Transporter in Glioma Cell Lines
Authors: Namgail Tundup , Kumar Dinesh , Vashistha Vidhi , Aquib Ahmed , Yadav Ajay
Published: July 2020

Glioblastoma multiforme (GBM) is classified as WHO grade IV Astrocytoma & is the most common highly aggressive form of primary brain tumor. Garde IV tumor are highly recurrent even after treatment, with patient survival rate is less than two years from the time of diagnosis. This might be due to overexpression of one of the factor such as ATP-binding cassette transporters (ABC transporters) responsible for drug resistance. ABCC transporter family a member of ABC transporter was found to mostly responsible for multi drug resistance (MDR) in cancer cells. On the other hand, heterogeneous nuclear ribonucleoprotein (hnRNP) an alternative splicing factor play different role in various cellular process such as nucleic acid metabolism, transcription and translation regulation, among them hnRNPA1 is best studied and its aberrant deregulation favor development of cancer. This study was focused on to study the function of hnRNPA1 in the expression analysis of ABCC transporter (responsible for MDR) in glioma cell lines. The expression of ABCC transporter (ABCC4 and ABCC6) gene was examined in two glioma cell line i.e. U87MG and T98G in normal and knockdown two alternative variants of hnRNPA1 by Quantitative Realtime PCR and Reverse Transcription PCR. We found that ABCC4 was significantly overexpressed in hnRNPA1 Variant 2 knockdown cells (si hnRNPA1 V2) in U87 (3-fold) and in T98G (18.34-fold), While hnRNPA1 Variant 1 knockdown cells (hnRNPA1 V1i) does not shows any significant effect. Further, the expression of ABCC6 was decreased in both hnRNPA1 V1i (0.40-fold) and hnRNPA1 V2i (0.48-fold) in U87. Reverse transcription based result were complemented with normal PCR based detection strategy after running in agarose gel for U87MG and T98G glioma cell line. Finally, this result indicates that hnRNPA1 an alternative splicing factor regulate the expression of ABCC4 and ABCC6 transporter which are responsible for multiple drug resistance in cancer. This information will help in future for the development of an alternative method for the treatment of drug resistance cases in brain tumor and other tumors by targeting hnRNPA1 splicing factor.
Dependency of COVID-19 Spread on Temperature in Rajasthan
Authors: SOLANKI MUKUL
Published: July 2020

To evaluate the dependency of COVID-19 on the temperature, by analyzing secondary data, considering temperature as the only dependent environmental factor. Taking the mean temperature and number of cases from 02nd March to 26th May 2020. We find out the relationship between the number of cases of COVID-19 and mean temperature in Rajasthan by finding the mean and percentage increase of both, and we evaluate that there is no any effect of temperature on coronavirus disease cases in Rajasthan there may be some other environmental factor and extraneous factors which may affect the number of cases.
Tasawuf dan Budaya Populer: Studi atas Pengajian Online Kitab al-Hikam di Facebook oleh Ulil Abshar Abdalla
Authors: Sakhok Jazilus , Munandar Siswoyo Aris , Ladzidzah Ibtisaamatin
Published: July 2020

This study discusses Sufism and Popular Culture: A Study of the Online Study of the Al-Hikam Book on Facebook by Ulil Abshar Abdalla. The author's aims of discusses this because in the modern era peoples use this gadget many are because of the need of the spiritually, but not most of them can actualize themselves to find it. In this case, the figure of Ulil Abshar Abdalla initiated a study in the modern era in the online sites, especially on Facebook as a way of transforming the values of Sufism. This study raised one of Ibn Athaillah's books, al-Hikam. The reason researchers chose this study:   first, this study is still rarely done by Sufism reviewers; second, the uniqueness that was built by Ulil Abshar Abdalla in conveying through facebook media and sharing its explanations to the laypeople and Sufism experts. The results of this determination are: first, revealing new ways or methods in presenting Sufism in this modern era; secondly, it found a relationship between Sufism and popular culture so that it can exist in the respective fields. Finally, Sufism can convey the wise messages in the book of al-Hikam in a wide circle.
A Case Report of Rapid Aseptic Intervertebral Disc Destruction After Lumbar Microdiscectomy
Authors: Clark DesRaj M. , Fredericks Donald R. , Wagner Scott C.
Published: July 2020

Case:

A previously healthy military recruit underwent routine microdiscectomy after lumbar disc herniation. After a period of improvement, he developed recurrent pain without repeat injury. Advanced imaging showing loss of marrow signal, and disc height was concerning for discitis. Inflammatory markers remained negative. Conservative treatment without antibiotics led to symptom resolution.

Conclusion:

This case demonstrates an unusual complication after lumbar microdiscectomy in a healthy individual. The recurrent symptoms and imaging changes were likely secondary to aseptic discitis and rapid degeneration rather than infection. A stepwise approach is critical for determining the cause of pain exacerbation after spinal procedures.
A qualitative study of stakeholder views on the effects of a sugar-sweetened beverages tax on the budgets, dietary intake, and health of lower and higher socioeconomic groups in the Netherlands
Authors: Djojosoeparto Sanne , Eykelenboom Michelle , Poelman Maartje , Stralen Maartje van , Renders Carry , Olthof Margreet , Steenhuis Ingrid , Kamphuis Carlijn
Published: July 2020

The authors have withdrawn this preprint due to author disagreement.
Prediction of higher thermoelectric performance in BiOCuSe by weakening electron-polar optical phonon scattering
Authors: Zhao Tianqi , Gibson Quinn , Daniels Luke , Slater Ben , Cora Furio
Published: July 2020

BiOCuSe is a promising thermoelectric material, but its applications are hindered by low carrier mobility. We use first principles calculations to analyse electron-phonon scattering mechanisms and evaluate their contributions to the thermoelectric figure of merit ZT. The combined scattering of carriers by polar optical (PO) and longitudinal acoustic (LA) phonons yields an intrinsic hole mobility of 32 cm2 V-1 s-1 at room temperature and a temperature power law of T-1.5, which agree well with experiments. We demonstrate that electron phonon scattering in the Cu-Se layer dominates at low T, while contributions from the Bi-O layer become increasingly significant at higher T. At room temperature, ZT is calculated to be 0.48 and can be improved by 30% through weakening PO phonon scattering in the Cu-Se layer. This finding agrees with the experimental observation that weakening the carrier-phonon interaction by Te substitution in the Cu-Se layer improves mobility and ZT. At high T, the figure of merit is improved by weakening phonon scattering in the Bi-O layer instead. The theoretical ZT limit of BiOCuSe is calculated to be 2.5 at 875 K.
LEACH-MTC: A Network Energy Optimization Algorithm Constraint as Moving Target Prediction
Authors: HU Zhen-tao , JIN Yong , Zhou Lin , WANG Chen , LI Ji-fang
Published: July 2020

When some nodes cooperatively track moving targets in wireless sensor network, some things including network working node selection and network energy consumption are influenced. Thus, this paper proposes an improved algorithm LEACH-MTC (LEACH with Moving Target Constraint) based on low energy adaptive clustering hierarchy protocol (LEACH). Firstly, based on the two-step linearization of the nonlinear dynamic model, the state of nonlinear moving target is predicted by the extended Kalman filter (EKF). Secondly, combining the state prediction of moving target and the performance of collaborative monitoring, this paper constructs an ellipse monitoring area of some working nodes to consist with the direction of the target movement. Subsequently, the node sleep strategy corresponding to the state prediction of moving target is designed. Finally, the cluster head selection strategy is proposed based on energy balance utilizing the state prediction of the moving target. Simulation results show that the proposed LEACH-MTC algorithm can not only ensure the real time consistency between the changing direction of area and the direction of target movement, but also increase the number of working nodes’ survival and reduce the network energy consumption.
Building Supramolecular Chirality in Organic Solar Cells Enables High-Performing Circularly Polarized Light Detection
Authors: Liu Lixuan , Yang Yang , Zhang Jianqi , Memon Waqar , Zhang Yajie , Wei Zhixiang
Published: July 2020

High-performing direct circularly polarized light (CPL) detectors are urgently needed for the development of chiral optoelectronics. We herein report the direct CPL detectors based on chiral organic small-molecule donor–fullerene acceptor bulk heterojunction organic solar cells (OSCs). By building supramolecular chirality in active layer of OSCs, the chiral OSC demonstrates its highest short-circuit current dissymmetry factor of 0.17 among state-of-the-art direct CPL detectors possessing intrinsic chirality. It demonstrates a significant difference in responsivity of 40 mA W− 1 upon opposite CPL illumination, four times higher than that of the best reported chiral perovskite CPL detectors to date. The association between the device metrics crucially relating to CPL detection and the anisotropic factor (g-factor) of the active layers revealed that supramolecular chirality is vital to the CPL detectability of OSCs. Given unique but ubiquitous observed supramolecular chirality induction and transfer in organic conjugated systems, highly sensitive CPL detectors based on chiral OSCs show great potential for promoting the development of practical direct CPL detection.
Target SARS-CoV-2: Computation of Binding energies with drugs of Dexamethasone/Umifenovir by Molecular Dynamics using OPLS-AA force field
Authors: Na Sk. Md , R M. Srinivasa
Published: July 2020

Molecular Dynamics simulation using Gromacs with OPLS-AA force field is performed for 100ns between SARS-CoV-2 main protease and Dexamethasone / Umifenovir drugs at 300 K/1 atm pressure. The trajectory of Root Mean Square Deviation (RMSD) and Radius of Gyration(Rg) emphasized the achievement of equilibrium and compactness. The drug-binding affinities on SARS-CoV-2 main protease are estimated via MM/PBSA method. The sign with magnitude of computed Gibbs free energy indicated the presence of strong interactions between SARS-CoV-2 and drugs of Dexamethasone / Umifenovir. The study revealed that the drug Dexamethasone is more effective over Umifenovir in binding SARS-CoV-2 main protease.
Identification of Key Genes and Evaluation of Their Prognosis Potential in Hepatocellular Carcinoma by Integrated Bioinformatics Analysis
Authors: Liu Jankun , liu zy , Li Wei , Pan Xinghua , Fan Zongjiang , Zhang Shurong
Published: July 2020

Background

Hepatocellular carcinoma (HCC) is a malignancy causing highly death rate in the world. Despite the development of treatment strategies for HCC, prognosis of this malignancy remains unsatisfactory. In this study, we aimed to identify the target genes associated with the prognosis of HCC patients.
Methods

Three expression profiles of HCC tissues were extracted from the Gene Expression Omnibus database to explore the differentially expressed genes (DEGs) using GEO2R method. Functional enrichment analysis was performed to reveal the biological characteristics of DEGs. Protein-protein interaction (PPI) network was constructed using Cytoscape software. The survival curve of identified DEGs were tested by Kaplan-Meier analysis.
Results

We identified 15 DEGs (CYP39A1, CYR61, FOS, FOXO1, GADD45B, ID1, IL1RAP, MT1M, PHLDA1, RND3, SDS, SOCS2, TAT, S100P, and SPINK1) in HCC tissues. Prognosis analysis showed that 4 DEGs (FOXO1,SPINK1༌SOCS2, and TAT) correlated with overall survival time of HCC patients, which might serve as therapeutic targets for HCC patients.
Conclusions

By integrated bioinformatics analysis, we proposed a novel way to reveal key genes that closely relate to HCC development.
Correlation Analysis Between Hematological Toxicity and Body Composition in Patients With Diffuse Large B Cell Lymphoma Treated With CHOP±R Regimen
Authors: Zhao Wenhao , Liu Xuelian , Liu Xiangliang , Yang Haimei , Ji Wei , Li Wei
Published: July 2020

Purpose: The tolerance of patients withdiffuse large B cell lymphoma（DLBCL） receiving CHOP±R regimen was significantly different, and grade 3~4 hematologic toxicity after chemotherapy in some patients resulted in prolonged hospital stay, increased risk of infection, delayed treatment, and directly or indirectly affected short-term efficacy and long-term prognosis. Lean body mass（LBM）and L3 skeletal muscle index （L3SMI）obtained from abdominal CT of DLBCL patients were analyzed to determine whether they could be used as independent predictors of hematological toxicity of CHOP± R regimen in DLBCL patients.Methods: The patients with DLBCL who underwent CHOP±R regimen at the Cancer Center of the First Hospital of Jilin University from January 2015 to November 2018 were retrospectively analyzed. The abdominal CT of the patient was analyzed by sliceOmatic5.0 software. The third lumbar disc planar imaging was selected, and two consecutive images were taken to calculate LBM and L3SMI. Single factor and multivariate analysis were performed on the correlation of LBM, L3SMIand chemotherapy-related grade 3~4 hematologic toxicity. The ROC curve was drawn to investigate the predictive value of various human indicators on the hematologic toxicity of grade 3~4 related to chemotherapy.Results: The L3 skeletal muscle index is associated with the occurrence of grade 3~4 hematologic toxicity (leukocyte and neutropenia) in patients with diffuse large B-cell lymphoma treated with CHOP±R regimen. Those with lower L3SMI are prone to grade 3~4 hematologic toxicity.LBM is associated with the occurrence of grade 3~4 hematologic toxicity (leukopenia) in patients with diffuse large B-cell lymphoma treated with CHOP±R regimen. This with lower LBM is prone to grade 3~4 hematologic toxicity.The L3 skeletal muscle index can be used as an independent predictor of grade 3~4 hematologic toxicity (leukocyte and neutropenia) in patients with diffuse large B-cell lymphoma treated with CHOP ± R regimen. The cut-off value can be defined as 39.91 cm2/m2.Conclusion: We can draw the following conclusions:The L3 skeletal muscle index is associated with the occurrence of grade 3~4 hematologic toxicity (leukocyte and neutropenia) in patients with diffuse large B-cell lymphoma treated with CHOP±R regimen. Those with lower L3SMI are prone to grade 3~4 hematologic toxicity.LBM is associated with the occurrence of grade 3~4 hematologic toxicity (leukopenia) in patients with diffuse large B-cell lymphoma treated with CHOP±R regimen. This with lower LBM is prone to grade 3~4 hematologic toxicity.The L3 skeletal muscle index can be used as an independent predictor of grade 3~4 hematologic toxicity (leukocyte and neutropenia) in patients with diffuse large B-cell lymphoma treated with CHOP ± R regimen. The cut-off value can be defined as 39.91 cm2/m 2.
Empagliflozin Ameliorates Leukocyte – Endothelium Cell Interactions and Inflammation in Type 2 Diabetic Patients
Authors: Iannantuoni Francesca , Canet Francisco , Marañon Aranzazu Martinez de , Lopez-Domenech Sandra , Vezza Teresa , Navarro Blanca , Sola Eva , Morillas Carlos , Rocha Milagros , Victor Victor M
Published: July 2020

Background: SGLT2 inhibitors (iSGLT2) such as empagliflozin can reduce cardiovascular risk in patients with type 2 diabetes, but the underlying molecular mechanisms are yet to be determined. In the present study we evaluate the effects of empagliflozin on anthropometric and endocrine parameters, leukocyte-endothelium interactions, adhesion molecules and NFkB-p65 transcription factor expression. Methods: Eighteen patients with type 2 diabetes were recruited for the study. Patients received 10 mg/day of empagliflozin according to standard clinical protocols and were followed-up during a 24-week period. Anthropometric and analytical measurements were performed at baseline, 12-weeks and 24-weeks. Interactions between polymorphonuclear leukocytes and human umbilical vein endothelial cells (HUVECs), serum levels of adhesion molecules (P-selectin, VCAM-1 and ICAM-1) and NFkB-p65 protein levels were measured. Results: We observed a decrease in body weight, BMI and HbA1C levels from 12 weeks of treatment, which had become more pronounced at 24 weeks and was accompanied by a significant reduction in waist circumference, glucose, and hs-CRP levels. Leukocyte-endothelium interactions were reduced due to an enhancement of leukocyte rolling velocity from 12 weeks onwards, together with a significant decrease in leukocyte rolling flux and adhesion at 24 weeks. Accordingly, a significant decrease in ICAM-1 levels and NFkB-p65 expression were observed. Conclusions: Empagliflozin reduced leukocyte-endothelium interactions, adhesion molecules and NFkB-p65 expression in type 2 diabetic patients after 24 weeks of treatment.
The Distribution Characteristic of Microbial Aerosols Inside a Nursery Pig House and the Respiratory Tract of Piglets
Authors: Tang Qian , Huang Kai , Liu Junze , Jin Xiaoming , Li ChunMei
Published: July 2020

Background: The particulate matter (PM) is a carrier of many substances. Microorganisms are vital constituents contained in PM and the kinds and concentration are closely connected to human health and animal production. This study was aimed to investigate the distribution characteristics of microbial aerosols inside the pig house and as well as in the respiratory tract of pigs. The environment inside a nursery pig house was monitored in winter, including temperature, relative humidity, TSP, PM10, PM2.5, NH3, CO2, CO and NO. The concentrations of airborne culturable bacteria, fungi and Escherichia coli were detected. Then 16 S rRNA sequencing technology was applied to identify bacteria contained in different sized PM and the bacteria in the respiratory tract of piglets.Results: The results showed that the concentration of airborne culturable bacteria inside the pig house was significantly higher than that outside. The concentration of airborne culturable bacteria was reduced with the decrease of the size of PM; the concentration of airborne culturable fungi was mostly distributed in the size of 2.1-3.3 μm and 1.1-2.1 μm PM; most airborne culturable Escherichia coli were distributed in the size of >7.0 μm and 2.1-3.3 μm. Besides, these three types of microbial aerosols did not exhibit significant change during different time points. The 16 S rRNA results showed that the bacteria contained in PM had high similarity with that in the respiratory tract of pigs. The bacteria assemblage in the size of 1.1-3.3 μm PM had high similarity with that in the lower respiratory tract (bronchus and lung) of pigs. In addition, four potential pathogenic bacteria genera (Escherichia-Shigella, Streptococcus, Acinetobacter, Pseudomonas) were identified in the PM samples and the respiratory tract.Conclusions: These results will provide a significant scientific basis in exploring the potential risk of aerosol from animal houses for human and animal health.
Anti-fatigue Effects of Ginseng Antler Yam Tang Modulation of Oxidative Stress Signaling in a Mice Model
Authors: Miao Lei , Zhang Rongrong , Shao Shuai , Zhang Hongyin , Xiao Fengqin , Yi Chunguang , Zhao Daqing , yan mingming
Published: July 2020

Background: Ginseng Antler Yam Tang (GAYT), believe to invigorate “Qi” (vital energy), nourish “Blood” (body circulation) and engender “liquid” (body fluid), is a traditional Chinese medicine formula derived from the traditional prescription and Chinese traditional medicine partner theory. Methods: In this study, we aimed to evaluate the anti-fatigue effects of GAYT and its mechanisms are related to oxidative stress signaling using GAYT composition, in vitro and in vivo antioxidant, and biochemical index detection. Chemical components analysis of GAYT was performed by high performance liquid chromatography (HPLC) and ultraviolet spectrophotometry (UV). Results: The results show that the GAYT is rich in protein, total flavonoids, total polysaccharide and saponin. The mice model was treatment by GAYT (0.9, 1.8 and 3.6g/kg) for 4 weeks. GAYT treatment enhanced antioxidant activities. GAYT significantly enhances the exercise performance in weight-loaded swimming, rotating rod, and forced running test. Biochemical index levels showed that these effects were closely correlated with inhibiting the depletion of glycogen, blood lactic acid (LD) and adenosine triphosphate (ATP) stores, regulating oxidative stress-related parameters (superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malonaldehyde (MDA)) in serum and liver of mice. Moreover, the results show that the effects of GAYT may be related with its regulation on the activations of AMP-activated protein kinase and protein kinase B in liver of mice.Conclusions: GAYT can induce recovery from fatigue in mice via the activation of the AMPK and AKT/mTOR pathways. Provide a theoretical basis for the study of GAYT's anti-fatigue effect
Moving towards a competency-based curriculum: Information analysis of patients in geriatric and special care dentistry
Authors: Limpuangthip Nareudee , Komin Orapin , Tatiyapongpaiboon Teerawut
Published: July 2020

Background: There is no core competency for geriatric dentistry and special patient care (GSP) in Thailand. Therefore, the present GSP program was initially developed as a knowledge-based curriculum. The objectives of this study were to improve the present GSP program by analysing information of the patients attending the GSP clinic, and the proposed future GSP curriculum is discussed.Methods: The GSP curriculum used in 2016–2019 was reviewed. Information of the 130 patients attending the GSP clinic, and those who participated a maintenance recall was analysed. The patients were invited to participate a maintenance recall visit to have their general health and oral conditions reassessed.Results: Only 50% of the patients attended a maintenance recall to be assessed for their oral and general health. A model of the future curriculum was developed based on the patients’ information. The content descriptions and learning methods of the present GSP curriculum need modification. The oral health care for elderly and special care patients requires more extensive collaboration with multiple health professions. The program has to include more learning sites for students to gain more experience and skills, especially in managing dependent and frail elders. Conclusions: Information analysis of the targeted patients is essential to improve and transform a knowledge-based curriculum for geriatric and special patient care dentistry into a competency-based curriculum. The GSP curriculum are present as a model for other institutions to develop their own curriculum related to the oral health care of geriatric and special care patients. Oral health care in elderly and special care patients should encompass not only oral disease problems, but also behavior and social context.
The Role of M6A-related CBLL1 in the Prognosis and Immune Infiltration of Pan-cancer
Authors: Guo Qiang , Li Dan , Ji Yan-Mei , Guo Jialong
Published: July 2020

Background

The modification of N6-methyladenosine (m6A) plays an important role in physiology and disease progression. The relationship between the role of m6a-related gene CBLL1 in pan-carcinoma and tumor immune infiltrates has remained unknown.
Methods

To explore the expression level of CBLL1 methylation in pan-cancer in SMART database, and download mRNA expression, mutation and clinical data in UCSC database, to analyze the expression level of CBLL1, and the relationship between CBLL1 expression and clinicopathological features, prognosis, mutation and immune microenvironment in pan-cancer. CIBERSORT was used to analyze the relationship between the expression of CBLL1 and the infiltration of pan-carcinoma immune cells. The mRNA expression data of UCSC database were used to analyze the correlation between CBLL1 expression and pan-cancer immunomodulations, checkpoints and receptor molecules. Gene Set enrichment analysis (GSEA) revealed the possible mechanism of CBLL1 in the regulation of pan-cancer progression.
Results

The levels of CBLL1 methylation and mRNA expression in pan-cancer tissues were abnormal. The level of CBLL1 is related to the age, race, clinical stage and treatment effect of patients with pan-carcinoma and associated with the prognosis of patients with KIRC, LUSC, THCA, THYM, MESO, PRAD, STAD, and UVM. Univariate COX regression analysis showed that expression of CBLL1 was a risk factor for poor prognosis in patients with KICH, KIRC, LAML, THYM, KIRC, PCPG, OV, PRAD, STAD, GBM and UVM.The expression level of CBLL1 was correlated with BLCA, BRCA, COAD, LAML, LGG, LUAD, LUSC, SARC, STAD, THCA, THYM and UVM tumor mutational burden (TMB), and with ACC, BRCA, CESC, COAD, DLBC, HNSC, PRAD, READ, SARC, STAD, TGCT, THCA and UCEC microsatellite instability (MSI). The expression level of CBLL1 was correlated with pan-cancer stromal cells and immune cells. The expression of CBLL1 is related to pan-cancer immunomodulators, checkpoints and receptor molecules. GSEA found that CBLL1 may participate in the progression of pan-cancer through B cell receptor singaling pathway, mRNA binding, immunoglobulin receptor binding, Positive Regulation of cell cycle phase transition and other mechanisms.
Conclusions

CBLL1 is abnormally expressed in patients with pan-carcinoma, which is expected to be a biomarker for prognosis, mutation and immune infiltration in patients with pan-carcinoma.
High-fat diet-induced oxidative stress links increased colonisation of Lactobacillus sakei with its anti-inflammatory properties in obesity
Authors: Lee Jee-Yon , Bae Eunsoo , Kim Hwa Young , Lee Kang-Mu , Yoon Sang Sun , Lee Duk-Chul
Published: July 2020

Background: Obesity is one of the major public health problems related to various chronic health conditions, with steadily increasing prevalence worldwide. Lactobacillus provides various benefits to the host body; however, its role in obesity is unknown. Results: In this study, we found higher colonisation of Lactobacillus sakei species in obesity group, which in turn was related to increased reactive oxygen species (ROS) levels induced by higher fat intake. We isolated L. sakei ob4.1 strain from the faeces of one subject with obesity and compared its genetic and molecular features with those of L. sakei DSM 20017 strain. L. sakei ob4.1 showed higher catalase activity, which was regulated by oxidative stress at gene transcriptional levels. L. sakei ob4.1 maintained colon epithelial cell-adhesion ability under ROS stimulation, and live bacteria could decrease colon epithelial inflammation in a dose-dependent manner. Establishing a mouse model revealed high-fat diet-induced colon ROS to be associated with increased colonisation of L. sakei ob4.1 through catalase activity. Four-week supplementation with this strain could reduce colon inflammation effectively, though not so for body weight or ROS levels in high fat-fed mice. Conclusion: We, therefore, concluded that changes in host gut-oxidative stress levels could link high fat-induced obesity to increased colonisation of L. sakei ob4.1 , and this strain could be potent as anti-inflammatory probiotic in obese population with gut inflammation. Keywords Lactobacillus sakei; Obesity; Gut microbiome; Reactive oxygen stress; Inflammation; High fat diet
Topolins and Red Light Improve the Micropropagation Efficiency of Passion Fruit (Passiflora edulis Sims) ‘Tainung No. 1’
Authors: Chen Ying-Chun , Chang Chen , Lin Huey-Ling
Published: July 2020

Passion fruit is a commercial crop of economic importance worldwide, with recent increases in demand for high-quality plants for commercial production. Plant tissue culture is widely used for the mass propagation of many commercial crops, however its application on passion fruit is challenged by the problem of low reproducibility, leaf chlorosis, and growth retardation resulted from in vitro culture. The aim of this study was to evaluate the effects of cytokinins and light quality on in vitro culture of nodal segments of passion fruit ‘Tainung No. 1’. Three aromatic cytokinins were tested in a modified MS basal medium. The bud proliferation rates of segments initiated on a media containing 1 mg·L−1 meta-topolin riboside (mTR) or benzyladenine (BA) were not significantly different at the same concentration. Buds cultured on medium supplemented with mTR grew and elongated for 4 weeks, while buds on a medium containing BA formed rosettes. After transfer to a medium without plant growth regulators (PGRs), shoots rooted spontaneously within 8 weeks. Furthermore, the effects of continuous propagation under a high proportion of red light affected the subsequent plant growth. Red LED induced an increase in the chlorophyll content (2.71 mg·g−1) compared with other light qualities (1.05–2.63 mg·g−1) and improved plantlet quality. Acclimated plants were grown in the field, and the flower morphology and fruit set were of commercial quality. Findings showed that replacing BA with mTR as the main cytokinin and using a high proportion of red light during the tissue culture induction period produced high-quality plantlets in 3 months. This system is economical and will be further developed for the commercial propagation of passion fruit vines in the future.
Chronic obstructive pulmonary disease in organized groups
Authors: Tаshmetovа G. T. , Liverko I. V.
Published: July 2020

The objective: to study the prevalence of chronic obstructive pulmonary disease (COPD) in adult organized groups, to assess the structure and risk factors of its development.

Subjects and methods. 3,000 people of the organized population working at various enterprises in Tashkent aged 20 to 60 years (1,400 (46.7%) women and 1,600 (53.3%) men) underwent the contemporary pulmonological screening.

Results. The active pulmonological screening aimed to detect COPD among organized groups has been justified; examination of 3,000 people allowed detecting 198 (6.6%) new patients additionally to 65 cases that were already known at the time of screening. The chances of developing COPD increase with the presence of risk factors such as smoking, exposure to toxic substances and chemicals, and recurrent upper respiratory tract infections.
A legal obligation to adapt transboundary water agreements to climate change?
Authors: Jafroudi Maryam
Published: July 2020

Climate change disrupts the water cycle, jeopardises the security of water, food, and energy systems, and forces states to rethink water management practices to adapt to the new hydrological realities. When states share a water resource, adaptation to climate change proves to be more burdensome, especially if the resource in question is governed by a transboundary water agreement that fails to incorporate flexibility. The focus of this paper is on transboundary water basins with more than two riparian states, where only part of the co-riparian states are party to a transboundary water agreement. The paper discusses whether the recognition of the principle of equitable and reasonable use of water as a principle of customary international law creates an obligation for the party states to such agreements to adapt the terms of their arrangement to climate change. It argues that the principle of equitable and reasonable use of water establishes an obligation of conduct for the states to respect non-parties' riparian rights to a fair share of beneficial uses of water and protect the environment. Therefore, the nature of disruptions caused by climate change may make it imperative for the party states to take all possible measures that allow them to modify their water practices under an agreement to the implications of climate change in order to comply with the principle of equitable and reasonable use of water.
Análise do disclosure das provisões e dos passivos contingentes: um estudo de caso das empresas aéreas listadas na B3 em relação aderência ao CPC 25
Authors: Bissi de Oliveira Renata , César Oliveira Camelo Augusto , Do Prado Daciê Franciele , Anzilago Marcielle
Published: July 2020

Objetivo: Considerando que o setor de aviação civil brasileiro registrou representativa participação no PIB brasileiro e tem gerado significativa contribuição no valor agregado para economia, este artigo tem como objetivo analisar o disclosure nas demonstrações financeiras das empresas Azul Linhas Aéreas Brasileiras S. A., Gol Linhas Aéreas Inteligentes S. A. e Latam Airlines Group S. A. a partir do enfoque teórico e normativo do CPC 25 no período de 2013 a 2018.

Metodologia: Realizou-se uma pesquisa qualitativa e descritiva, onde se analisou como ocorre a divulgação das Provisões e Passivos Contingentes apresentadas nas demonstrações financeiras das companhias aéreas brasileiras listadas no Brasil, Bolsa, Bovespa (B3) – Azul Linhas Aéreas Brasileiras S. A., Gol Linhas Aéreas Inteligentes S. A. e Latam Airlines Group S. A., no período de 2013 a 2018. A técnica utilizada foi a análise de conteúdo, onde buscou-se verificar, primeiramente, como são classificadas as provisões e os passivos contingentes divulgadas pelas companhias de linhas aéreas. Em seguida, se apurou o quanto cada categoria representa (em valores) em relação as provisões e passivos contingentes divulgados.  

Resultados: Os resultados apontam que as informações ainda são falhas em relação à aderência às orientações do CPC 25 (2009) quanto à evidenciação de passivos contingentes, fato que pode representar uma barreira na análise da transparência dos dados e das informações aos potenciais usuários da informação contábil. Quanto às provisões, observou-se maior frequência de divulgação de informações que se referem a tributos, área cível, trabalhista e devolução de aeronaves.

Contribuições do Estudo: Esse estudo permite analisar aspectos relativos à natureza, reconhecimento e mensuração das provisões e passivos contingentes, sobretudo, em relação à transparência na divulgação e classificação desses itens, estimulando a diminuição da assimetria informacional dentre os usuários das informações contábeis.
Avaliação comparativa, por país, da situação econômico-financeira de bancos islâmicos
Authors: Jarbou Tariq , Niyama Jorge Katsumi
Published: July 2020

Objetivo: Este estudo visa promover uma avaliação comparativa por país da situação econômico-financeira de bancos islâmicos.

Metodologia: Foi utilizada uma abordagem analítica descritiva e documental. Realizou-se uma coleta de dados sobre indicadores financeiros de bancos islâmicos em 21 países e procedeu-se a análise desses dados à luz das normas recomendadas pelo Conselho de Serviços Financeiros Islâmicos (IFSB) e pelo Comitê de Supervisão Bancária de Basileia (BCBS). A amostra foi composta pelos países com dados disponíveis na base de dados do IFSB (PSIFIs). Os dados referem-se ao segundo trimestre de 2018 agrupados por países. Uma estatística descritiva simples, na forma de figuras, foi utilizada para analisar os dados coletados.

Resultados: Este documento ressalta que 90% das instituições financeiras islâmicas (IFIs) nos países da amostra calculam o índice de adequação de capital (CAR) de acordo com as recomendações do BCBS. Além disso, 95% apresentam CAR superior a 8% (o mínimo estabelecido pelo BCBS e IFSB). Portanto, 95% dos países possuem sistemas financeiros seguros em termos da solvência bancária. Em termos de qualidade de ativos, o sistema financeiro islâmico em Omã apresentou a melhor qualidade no gerenciamento dos seus recursos. O Sudão demonstrou os maiores índices de rentabilidade em seus bancos islâmicos. Na análise de liquidez, não foi possível identificar com precisão qual é o país de melhor índice de liquidez no curto e longo prazo uma vez que mais de 60% dos países não apresentaram dados suficientes. Em termos de ativos líquidos sobre o total de ativos, e o cumprimento de obrigações a curto prazo, os sistemas financeiros islâmicos do Egito e do Afeganistão, respectivamente, lideraram com melhores índices de liquidez da amostra, mostrando capacidade e segurança para cumprir suas obrigações.

Contribuições do Estudo: Identificar os países com maior/menor risco considerando os requerimentos e recomendações do IFSB e do BCBS.
Behavior of budget expenditures during the election period: an analysis in panel data in the Brazilian municipalities
Authors: Gilberto Crispim  , Luiz Alberton  , Celma Duque Ferreira  , Jorge Expedito de Gusmão Lopes
Published: July 2020

Objective: From the political budget cycle (PDC) lens, we investigated the behavior of committed expenditures, investment spending and borrowing in the electoral period, in Brazilian municipalities with a population of 50,000 or more, in the period 2000-2016, comprising 353 clusters and 6001 observations. It was also investigated whether these municipalities meet the legal requirements on the use of budget resources in an election year. The study was motivated by the lack of consensus in the literature on the subject in question.

Methodology: Four research hypotheses were built to assist in achieving the proposed objectives. Data were collected from IBGE, TSE and STN websites. Data were analyzed using the balanced panel data method, grouped by municipality and region, with Tobit regression application and Hausman, Breusch-Pagan Lagrange and F de Chow robustness tests. This method covered confidence with the interval of 99%, 77%, 79% and 84% explaining behavior between the variables.

Results: The results show that there were no changes in expenditure committed before, during and after the election period, regardless of party equality and two-round election, unlike the results of some studies. As for spending on investments and borrowing in the election period, the study suggests an average increase of 9% and 68% respectively, and this is more intense when there is party equality between municipal and state governments, especially in the process of reelection. Regarding compliance with legal requirements on the use of budgetary resources in an election year, the study indicates that local governments do not comply with regulatory requirements.

Contributions of the study: contributes to the specific literature, because it presents robust results on the lack of consensus on the subject in question, as well as for future research involving municipalities with smaller population and comparison between regions and countries.
PRÁTICAS ASSISTENCIAIS PARA SEGURANÇA DO PACIENTE NO TRANSPORTE INTRA-HOSPITALAR
Authors: Oliveira Adrielly Silva de , Bhenson Tavares Barbosa Malom , Antônio da Silva Gustavo , César Bernardino da Silva Júlio , Lais Alves Batista de Oliveira Hellenara , Augusto Lopes Oliveira Diego , Marinho Silva Barbosa Lidiane , De Carvalho Silva Cintia
Published: July 2020

Introdução: O risco ao paciente durante o transporte pode ser minimizado por meio de um planejamento cuidadoso, qualificação do pessoal responsável pelo transporte e seleção de equipamentos adequados. Dessa forma, possíveis ocorrências podem ser facilmente tratadas se houver uma equipe multiprofissional disponível para administrar qualquer complicação possível durante o procedimento. Objetivo: Elucidar o processo de transporte intra-hospitalar e sua relação com a segurança do paciente. Método: Estudo bibliográfico, descritivo, do tipo revisão integrativa no período de 2008 a 2017, nas bases de dados IBECS, LILACS e BDENF. Analisaram-se os estudos a partir da leitura dos títulos, resumos e dos artigos completos que respondessem o objetivo proposto e as sínteses dos resultados dos 4 artigos selecionados apresentaram-se de forma descritivas em figuras. Consideraram-se as categorias temáticas que emergiram da Técnica de Análise de Conteúdo após a análise dos artigos. Resultados: Observou-se nos estudos selecionados que o transporte do paciente envolve riscos fisiológicos e instabilidade hemodinâmica, sendo necessário medidas de segurança do paciente por meio de um planejamento cuidadoso, qualificação do pessoal e seleção de equipamentos adequados. Conclusões: Recomenda-se a padronização das ações dos profissionais envolvidos no transporte e a previsão e provisão dos equipamentos necessários para monitorização clínica do paciente, minimizando os eventos adversos e obtendo-se a excelência do atendimento e segurança do paciente. Destaca-se a necessidade de novos estudos que implementem novos protocolos assistenciais para a segurança do paciente no transporte intra-hospitalar.
QUALIDADE DA ASSISTÊNCIA AO PARTO E PÓS-PARTO NA PERCEPÇÃO DE USUÁRIAS DA ATENÇÃO PRIMÁRIA À SAÚDE
Authors: Silva Magda Jaqueline Santos da , Silva Tamires de Sales da , Souza Dandara Rayssa Silva de , Souza Ana Mayara Gomes de , Ferreira Tainara Lôrena dos Santos , Andrade Fábia Barbosa de
Published: July 2020

Introdução: considerando a importância do momento do parto/nascimento, espera-se que este processo seja realizado de forma adequada, tendo à disposição uma assistência de qualidade, de modo que a mulher tenha seus direitos garantidos. Objetivo: caracterizar e avaliar a qualidade da assistência prestada no parto e pós-parto, a partir da visão de mães, usuárias dos serviços de Atenção Primária à Saúde, com enfoque nos aspectos relativos à humanização. Metodologia: trata-se de uma pesquisa quantitativa o tipo exploratória e descritiva, com abordagem avaliativa, realizada na cidade de Santa Cruz no estado do Rio Grande do Norte.  A coleta de dados se deu no período de novembro de 2012 a fevereiro de 2013, onde foram entrevistadas 200 mulheres. Resultados: 60,5% tiveram parto vaginal; 66,5% tiveram um acompanhante de sua escolha durante o parto; 90,0% das mães afirmaram ter ficado com seus filhos no quarto após o parto; 60,5% avaliaram a qualidade do atendimento no parto como bom; 54,5% buscaram o serviço de saúde na primeira semana após o nascimento. Conclusão: Conclui-se, que a assistência prestada à mulher no parto e pós-parto foi satisfatória do ponto de vista da maioria das entrevistadas no presente estudo.
Design and Synthesis of an Aniline Derivative with Biological Activity on Heart Failure
Authors: Alejandra Garcimarero-Espino E. , Lauro Figueroa-Valverde , Marcela Rosas-Nexticapa , Maria Lopez-Ramos , Francisco Diaz Cedillo , Virginia Mateu-Armand , Yazmin Ortiz-Ake
Published: July 2020

Background:

Several compounds have been synthesized as a therapeutic alternative for
heart failure; however, its preparation requires special conditions.

Objective:

The aim of this study, was to synthesize some aniline derivatives (4-9) from 3-
ethynylaniline to evaluate their biological activity against heart failure.

Methods:

The synthesis of aniline derivatives involved a series of reactions such as etherification,
addition, and cyclization. The structure of all compounds obtained was confirmed by spectroscopic
and spectrometric methods. In addition, to evaluate the biological activity of compounds, an
ischemia/reperfusion injury model was used.

Results:

The results showed that compound 8 decreases heart failure, which translates into a
decrease in the infarction area compared to compounds 4-7 and 9.

Conclusion:

This study reports a facile method for the preparation of aniline derivatives. This method
offers some advantages such as; a simple procedure, low cost, and easy work up. In addition,
compound 8 showed an interesting biological activity against heart failure. This phenomenon is
particularly interesting because the biological activity induced by this compound could involve a
molecular mechanism that is different from other drugs used for the treatment of heart failure.