bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Figure 1 from Dual Inhibition of PI3K and mTOR Mitigates Compensatory AKT Activation and Improves Tamoxifen Response in Breast Cancer
Authors: Chen Xiaosong , Zhao Meizhong , Hao Mingang , Sun Xueqing , Wang Jinglong , Mao Yan , Zu Lidong , Liu Junjun , Shen Yandong , Wang Jianhua , Shen Kunwei
Published: April 2023

PDF file - 588K, Effects of single agent of tamoxifen, everolimus, or PI3K inhibitors (LY294002 or BKM120, BEZ-235) on MCF-7 and BT474 breast cancer cell lines after 48h and 72h treatment.
Supplementary Figures 1-5 from Epithelial-Specific Deletion of 11β-HSD2 Hinders Apcmin/+ Mouse Tumorigenesis
Authors: Jiang Li , Yang Shilin , Yin Huiyong , Fan Xiaofeng , Wang Suwan , Yao Bing , Pozzi Ambra , Chen Xiaoping , Harris Raymond C. , Zhang Ming-Zhi
Published: April 2023

PDF - 386KB, S1 Dual recombinase strategy for producing a "clean" loxP-floxed Hsd11b2. S2. The targeted events were confirmed with PCR and Southern analysis in F1 mice. S3. Inhibition of the COX-2 pathway had no effect on the activity of retinoblastoma protein and mTOR. S4. Glucocorticoids increased the activity of the tumor suppressor retinoblastoma protein and decreased the activity of mTOR pathway in cultured colonic cancer cells. S5. Inhibition of mTOR activity or inhibition of COX-2 activity did not affect the expression levels of p53 and p21 and REDD1.
Supplementary Table 4 from The Impact of miRNA-Based Molecular Diagnostics and Treatment of NRF2-Stabilized Tumors
Authors: Yamamoto Shinsuke , Inoue Jun , Kawano Tatsuyuki , Kozaki Ken-ichi , Omura Ken , Inazawa Johji
Published: April 2023

XLSX file - 29K, Primers for human NQO1 promoter region.
Supplementary Table 1 from The Impact of miRNA-Based Molecular Diagnostics and Treatment of NRF2-Stabilized Tumors
Authors: Yamamoto Shinsuke , Inoue Jun , Kawano Tatsuyuki , Kozaki Ken-ichi , Omura Ken , Inazawa Johji
Published: April 2023

XLSX file - 94K, Result of function-based screening of miRNAs negatively regulating transcriptional activity of NRF2 in HeLa cells used in Pre-miR? miRNA Precursor Library - Human V4 (Ambion).
Supplementary Figure 4 from The Impact of miRNA-Based Molecular Diagnostics and Treatment of NRF2-Stabilized Tumors
Authors: Yamamoto Shinsuke , Inoue Jun , Kawano Tatsuyuki , Kozaki Ken-ichi , Omura Ken , Inazawa Johji
Published: April 2023

PDF file - 148K, Expression analysis of 4 miRNAs in ESCC samples.
Supplementary Figure 3 from The Impact of miRNA-Based Molecular Diagnostics and Treatment of NRF2-Stabilized Tumors
Authors: Yamamoto Shinsuke , Inoue Jun , Kawano Tatsuyuki , Kozaki Ken-ichi , Omura Ken , Inazawa Johji
Published: April 2023

PDF file - 86K, Expression analysis for NRF2 expression in miRNA-transfected cells.
Supplementary Figure 3 from Molecular Dissection of AKT Activation in Lung Cancer Cell Lines
Authors: Guo Yanan , Du Jinyan , Kwiatkowski David J.
Published: April 2023

PDF file - 1702K, The activation of AKT in HCC2429 cells is EGFR-independent.
Supplementary Tables 1 - 2 from RASEF is a Novel Diagnostic Biomarker and a Therapeutic Target for Lung Cancer
Authors: Oshita Hideto , Nishino Ryohei , Takano Atsushi , Fujitomo Takashi , Aragaki Masato , Kato Tatsuya , Akiyama Hirohiko , Tsuchiya Eiju , Kohno Nobuoki , Nakamura Yusuke , Daigo Yataro
Published: April 2023

PDF file - 113K, Supplementary Table S1. Lung cancer cell lines used in analysis. Supplementary Table S2. Background of lung cancer patients analyzed by tissue microarray (n=341).
Supplementary Figures 1 - 6 from RASEF is a Novel Diagnostic Biomarker and a Therapeutic Target for Lung Cancer
Authors: Oshita Hideto , Nishino Ryohei , Takano Atsushi , Fujitomo Takashi , Aragaki Masato , Kato Tatsuya , Akiyama Hirohiko , Tsuchiya Eiju , Kohno Nobuoki , Nakamura Yusuke , Daigo Yataro
Published: April 2023

PDF file - 2714K, S1. Western blot analysis with anti-RASEF antibody after phosphatase treatment of NCI-H2170 cells. S2. Kaplan-Meier analysis of tumor-specific survival of surgically treated NSCLC patients (n = 341) according to expression levels of RASEF. Markers on survival curves mean censored cases. S3. Kaplan-Meier analysis of tumor-specific survival of another set of NSCLC patients (n = 243) who underwent resection of primary tumors (P= 0.0382 by log-rank test). Makers on survival curves mean censored cases. S4. Statistical analysis of colony formation assay in Figure 3C. S5. The correlation between RASEF expression and ERK1/2 phosphorylation in lung cancer cells. S6. Immunohistochemistry on the serial sections of clinical lung cancer tissues using anti-RASEF antibody and anti-phosphorylated ERK1/2 antibody.
Supplementary Figure Legends from CYP1A1 Regulates Breast Cancer Proliferation and Survival
Authors: Rodriguez Mariangellys , Potter David A.
Published: April 2023

Supplementary Figure Legends- PDF file 57K, Legends for Supplementary Figures 1 and 2
Supplementary Figure 1 from CYP1A1 Regulates Breast Cancer Proliferation and Survival
Authors: Rodriguez Mariangellys , Potter David A.
Published: April 2023

Supplementary Figure 1 - PDF file 103K, Effect of AhRsiRNA on the growth of breast cancer cells
Supplementary Figure Legend from Activation of the NF-κB Pathway by the STAT3 Inhibitor JSI-124 in Human Glioblastoma Cells
Authors: McFarland Braden C. , Gray G. Kenneth , Nozell Susan E. , Hong Suk W. , Benveniste Etty N.
Published: April 2023

PDF file - 79K
Supplementary Figure 3 from CDCP1 Regulates the Function of MT1-MMP and Invadopodia-Mediated Invasion of Cancer Cells
Authors: Miyazawa Yuri , Uekita Takamasa , Ito Yuumi , Seiki Motoharu , Yamaguchi Hideki , Sakai Ryuichi
Published: April 2023

PDF File-139K, CDCP1 is not affecting MT1-MMP expression and pro-MT1-MMP activation by processing.
Supplementary Figure 3 from HER-2/neu Mediates Oncogenic Transformation via Altered CREB Expression and Function
Authors: Steven André , Leisz Sandra , Massa Chiara , Iezzi Manuela , Lattanzio Rossano , Lamolinara Alessia , Bukur Jürgen , Müller Anja , Hiebl Bernhard , Holzhausen Hans-Jürgen , Seliger Barbara
Published: April 2023

PDF file - 3024K, Time- and dose-dependent effects of KG-501 on the CREB expression and activity of HER-2/neu+ cells. (A) mRNA expression of CREB, CBP, bcl2 and bcl-xL was determined over time upon treatment with 10 �M KG-501 using RT-PCR followed by agarose electrophoresis as described in Materials and Methods. Expression of β-actin served as control. (B) HER-2/neu+ cells were incubated with KG-501 for 48 h at 37{degree sign}C and analysed as described in Materials and Methods. Western blotting using a phospho(Ser-133) mAb was performed and Beta-actin mAb served as the control. The right panel demonstrate the quantified densitometry data (right) are means �SD (n = 3). (C) CFSE-labeled HER-2/neu+ cells were incubated with KG-501 and fluorescence was determined 48 h later on a FACsCalibur. Red line, untreated cells.
Supplementary Figure 2 from HER-2/neu Mediates Oncogenic Transformation via Altered CREB Expression and Function
Authors: Steven André , Leisz Sandra , Massa Chiara , Iezzi Manuela , Lattanzio Rossano , Lamolinara Alessia , Bukur Jürgen , Müller Anja , Hiebl Bernhard , Holzhausen Hans-Jürgen , Seliger Barbara
Published: April 2023

PDF file - 1836K, Effects of shCREB constructs on CREB protein expression. (A) Efficacy of shCREB knock-down (shCREB-HER-2/neu+ cells) was analyzed using an NC control plasmid and four different shRNA plasmid contructs targeting murine CREB1. The following shRNA sequences were amplified and cloned into a pClip plasmid construct containing a puromycin resistance gene: plasmid #1: TCAGCCGGGTACTACCATTCT; plasmid #2: ACAGGGAGGCAGCAAGAGAAT, plasmid #3: AAGTCCAAACAGTTCAGATTT and plasmid #4: TGCTCCCACTGTAACCTTAGT. Plasmid NC (GGAATCTCATTCGATGCATAC) served as a nonsense control. These plasmids were transfected into HER-2/neu+ cells and CREB protein expression was determined by Western blotting as described in Materials and Methods using an anti-CREB-specific antibody. Arrows point to plasmid constructs that were further used in this study. (B) mRNA expression of ATF-1 and CREM was analyzed in HER-2/neu+ cells and shCREB-HER-2/neu+ cells using q-PCR. Data are means plus-minus SD (n = 3).
Supplementary Methods from Hematopoietic Expression of Oncogenic BRAF Promotes Aberrant Growth of Monocyte-Lineage Cells Resistant to PLX4720
Authors: Kamata Tamihiro , Dankort David , Kang Jing , Giblett Susan , Pritchard Catrin A. , McMahon Martin , Leavitt Andrew D.
Published: April 2023

PDF file - 21K
Supplementary Figure 7 from Hematopoietic Expression of Oncogenic BRAF Promotes Aberrant Growth of Monocyte-Lineage Cells Resistant to PLX4720
Authors: Kamata Tamihiro , Dankort David , Kang Jing , Giblett Susan , Pritchard Catrin A. , McMahon Martin , Leavitt Andrew D.
Published: April 2023

PDF file - 97K, S7. Sequence data from genomic DNA extracted from the SigM5 cell line.
Supplementary Figure 5 from Hematopoietic Expression of Oncogenic BRAF Promotes Aberrant Growth of Monocyte-Lineage Cells Resistant to PLX4720
Authors: Kamata Tamihiro , Dankort David , Kang Jing , Giblett Susan , Pritchard Catrin A. , McMahon Martin , Leavitt Andrew D.
Published: April 2023

PDF file - 697K, S5. In vitro liquid culture.
Supplementary Figure 2 from Hematopoietic Expression of Oncogenic BRAF Promotes Aberrant Growth of Monocyte-Lineage Cells Resistant to PLX4720
Authors: Kamata Tamihiro , Dankort David , Kang Jing , Giblett Susan , Pritchard Catrin A. , McMahon Martin , Leavitt Andrew D.
Published: April 2023

PDF file - 426K, Histological analysis of lung in primary mice.
Supplementary Figure 2 from EGFR Inhibition Induces Proinflammatory Cytokines via NOX4 in HNSCC
Authors: Fletcher Elise V.M. , Love-Homan Laurie , Sobhakumari Arya , Feddersen Charlotte R. , Koch Adam T. , Goel Apollina , Simons Andrean L.
Published: April 2023

PDF file - 170K, EGFR knockout induced IL-6 protein expression in FaDu cells.
Supplementary Figure 4 from miRNA-145 Targets v-ets Erythroblastosis Virus E26 Oncogene Homolog 1 to Suppress the Invasion, Metastasis, and Angiogenesis of Gastric Cancer Cells
Authors: Zheng Liduan , Pu Jiarui , Qi Teng , Qi Meng , Li Dan , Xiang Xuan , Huang Kai , Tong Qiangsong
Published: April 2023

PDF file - 15K, Effects of Ets1 knockdown on the proliferation of gastric cancer cells
Supplementary Figure 2 from miRNA-145 Targets v-ets Erythroblastosis Virus E26 Oncogene Homolog 1 to Suppress the Invasion, Metastasis, and Angiogenesis of Gastric Cancer Cells
Authors: Zheng Liduan , Pu Jiarui , Qi Teng , Qi Meng , Li Dan , Xiang Xuan , Huang Kai , Tong Qiangsong
Published: April 2023

PDF file - 16K, Ets1 regulated MMP-1 and MMP-9 in gastric cancer cells
Supplementary Materials and Methods from A Novel Regulatory Mechanism of Pim-3 Kinase Stability and Its Involvement in Pancreatic Cancer Progression
Authors: Zhang Fei , Liu Bin , Wang Zhen , Yu Xian-Jun , Ni Quan-Xing , Yang Wen-Tao , Mukaida Naofumi , Li Ying-Yi
Published: April 2023

PDF - 30KB, Supplementary Materials an Methods including antibody, patient and tissue samples, and scoring of TCTP, and a Pim-3 in human pancreatic cancer tissues section.
Supplementary Figure 1 from A Novel Regulatory Mechanism of Pim-3 Kinase Stability and Its Involvement in Pancreatic Cancer Progression
Authors: Zhang Fei , Liu Bin , Wang Zhen , Yu Xian-Jun , Ni Quan-Xing , Yang Wen-Tao , Mukaida Naofumi , Li Ying-Yi
Published: April 2023

PDF - 59KB, Quantitative RT-PCR analyses for Pim-1, Pim-2, and Pim-3.
Supplementary Figure Legend from PKCι Maintains a Tumor-initiating Cell Phenotype That Is Required for Ovarian Tumorigenesis
Authors: Wang Yin , Hill Kristen S. , Fields Alan P.
Published: April 2023

PDF file - 75K
Supplementary Figure 1 from PKCι Maintains a Tumor-initiating Cell Phenotype That Is Required for Ovarian Tumorigenesis
Authors: Wang Yin , Hill Kristen S. , Fields Alan P.
Published: April 2023

PDF file - 71K, S1. Expression of exogenous PKCι rescues the effects of PKCι RNAi on transformed growth of ovarian oncosphere cells.
Supplementary Figure Legends from PGE2-Driven Expression of c-Myc and OncomiR-17-92 Contributes to Apoptosis Resistance in NSCLC
Authors: Krysan Kostyantyn , Kusko Rebecca , Grogan Tristan , O'Hearn James , Reckamp Karen L. , Walser Tonya C. , Garon Edward B. , Lenburg Marc E. , Sharma Sherven , Spira Avrum E. , Elashoff David , Dubinett Steven M.
Published: April 2023

PDF file - 38K
Supplementary Figure 1 from PGE2-Driven Expression of c-Myc and OncomiR-17-92 Contributes to Apoptosis Resistance in NSCLC
Authors: Krysan Kostyantyn , Kusko Rebecca , Grogan Tristan , O'Hearn James , Reckamp Karen L. , Walser Tonya C. , Garon Edward B. , Lenburg Marc E. , Sharma Sherven , Spira Avrum E. , Elashoff David , Dubinett Steven M.
Published: April 2023

PDF file - 37K, Stable knockdown of c-myc expression by shRNA in NSCLC cells.
Supplementary Figure 5 from Towards Sustained Silencing of HER2/neu in Cancer By Epigenetic Editing
Authors: Falahi Fahimeh , Huisman Christian , Kazemier Hinke G. , van der Vlies Pieter , Kok Klaas , Hospers Geke A.P. , Rots Marianne G.
Published: April 2023

PDF file - 48K, Her2/neu downregulation status in MCF7 by pMX-E2C fused to histone methyltransferase domains.
Supplementary Figure 1 from Towards Sustained Silencing of HER2/neu in Cancer By Epigenetic Editing
Authors: Falahi Fahimeh , Huisman Christian , Kazemier Hinke G. , van der Vlies Pieter , Kok Klaas , Hospers Geke A.P. , Rots Marianne G.
Published: April 2023

PDF file - 56K, Her2/neu Zinc Finger Protein fused to VP64 does not have any effect on an irrelevant gene.
Supplementary Figure 7 from The HER2- and Heregulin β1 (HRG)–Inducible TNFR Superfamily Member Fn14 Promotes HRG-Driven Breast Cancer Cell Migration, Invasion, and MMP9 Expression
Authors: Asrani Kaushal , Keri Ruth A. , Galisteo Rebeca , Brown Sharron A.N. , Morgan Sarah J. , Ghosh Arundhati , Tran Nhan L. , Winkles Jeffrey A.
Published: April 2023

PDF file - 177K, Figure S7. HRG1-β1 stimulation increases MCF7/HER2-18 cell migration and invasion.
Supplementary Figure 4 from The HER2- and Heregulin β1 (HRG)–Inducible TNFR Superfamily Member Fn14 Promotes HRG-Driven Breast Cancer Cell Migration, Invasion, and MMP9 Expression
Authors: Asrani Kaushal , Keri Ruth A. , Galisteo Rebeca , Brown Sharron A.N. , Morgan Sarah J. , Ghosh Arundhati , Tran Nhan L. , Winkles Jeffrey A.
Published: April 2023

PDF file - 111K, Figure S4. Lapatinib treatment or HER2/HER3 siRNA co-transfection of MCF7/HER2-18 cells decreases Fn14 expression levels.
Table S5 from Mastermind-Like 3 Controls Proliferation and Differentiation in Neuroblastoma
Authors: Heynen Guus J.J.E. , Nevedomskaya Ekaterina , Palit Sander , Jagalur Basheer Noorjahan , Lieftink Cor , Schlicker Andreas , Zwart Wilbert , Bernards Rene , Bajpe Prashanth Kumar
Published: April 2023

Supplementary Table 5: Sources of publicly available ChIP-seq data for p300 and H3K27me1 in SK-N-SH
Supplementary Figure Legend from Androgen Receptor Promotes the Oncogenic Function of Overexpressed Jagged1 in Prostate Cancer by Enhancing Cyclin B1 Expression via Akt Phosphorylation
Authors: Yu Yongjiang , Zhang Yu , Guan Wenbin , Huang Tao , Kang Jian , Sheng Xujun , Qi Jun
Published: April 2023

PDF - 64K, Legends for Supplementary Figures 1 and 2 and Supplementary Tables 1 and 2
Table S3 from Mastermind-Like 3 Controls Proliferation and Differentiation in Neuroblastoma
Authors: Heynen Guus J.J.E. , Nevedomskaya Ekaterina , Palit Sander , Jagalur Basheer Noorjahan , Lieftink Cor , Schlicker Andreas , Zwart Wilbert , Bernards Rene , Bajpe Prashanth Kumar
Published: April 2023

Supplementary Table 3: List of RA target genes that show loss of activation upon RA treatment in MAML
Supplementary Figures 1-7 from Glutamine Transporters Are Targets of Multiple Oncogenic Signaling Pathways in Prostate Cancer
Authors: White Mark A. , Lin Chenchu , Rajapakshe Kimal , Dong Jianrong , Shi Yan , Tsouko Efrosini , Mukhopadhyay Ratna , Jasso Diana , Dawood Wajahat , Coarfa Cristian , Frigo Daniel E.
Published: April 2023

These supplementary data demonstrate the androgen biphasic effect (Fig. S1), that SLC1A4 and SLC1A5 are indirect AR target genes (Fig. S2), provide knockdown controls for siRNAs targeting SLC1A4 and SLC1A5 (Fig. S3) and shRNA targeting MYC in stable LNCaP cells (Fig. S4), that androgens increase MYC levels independently of mTOR (Fig. S5), rapamycin is not toxic (Fig. S6), and that MYC cannot promote glutamine transporter expression in PTEN wild-type cells (Fig. S7).
Supplementary Table 3 from CAF-Secreted Annexin A1 Induces Prostate Cancer Cells to Gain Stem Cell–like Features
Authors: Geary Lauren A. , Nash Kevin A. , Adisetiyo Helty , Liang Mengmeng , Liao Chun-Peng , Jeong Joseph H. , Zandi Ebrahim , Roy-Burman Pradip
Published: April 2023

XLS file - 23KB, Supplementary Table S3: Proteins.
Supplementary Information Supplementary Figures 1-3, Supplemental Table 1, Supplemental Figure and Table Legends from Identification of an “Exceptional Responder” Cell Line to MEK1 Inhibition: Clinical Implications for MEK-Targeted Therapy
Authors: Gannon Hugh S. , Kaplan Nathan , Tsherniak Aviad , Vazquez Francisca , Weir Barbara A. , Hahn William C. , Meyerson Matthew
Published: April 2023

Characteristics of the 21 lung cancer cell lines assayed in the Project Achilles data set (8). NSCLC = non-small cell lung cancer, LCLC = large cell lung cancer, SCLC = small cell lung cancer. S1. Each data point represents the normalized gene-level scores for ABL1 (ATARiS gene solution 2) in the 30 hematopoietic and lymphoid cell lines tested (8). The three most sensitive lines (indicated by red circles) are the only lines within this data set known to harbor the BCR-ABL1 translocation. S2. (A) Trametinib dose response curve after 6 days. Cell viability normalized to DMSO treated controls. (B) Refametinib dose response curve after 6 days. Cell viability normalized to DMSO treated controls. (C) PD0325901 dose response curve after 6 days. Cell viability normalized to DMSO treated controls. (D) Immunoblot of protein levels after 4-hour treatment with DMSO or 10 nM trametinib in each cancer cell line. S3. Xenograft tumor growth after NCI-H1437 subcutaneous injection in nude mice. Mice were treated daily with either vehicle 5% DMSO or 0.3mg/kg trametinib. For each tumor, the volume was normalized to its size at the start of drug treatment. Supplemental Table 1. Characteristics of the 21 lung cancer cell lines assayed in the Project Achilles data set (8). NSCLC = non-small cell lung cancer, LCLC = large cell lung cancer, SCLC = small cell lung cancer.
Supplementary Figure Legends from CAF-Secreted Annexin A1 Induces Prostate Cancer Cells to Gain Stem Cell–like Features
Authors: Geary Lauren A. , Nash Kevin A. , Adisetiyo Helty , Liang Mengmeng , Liao Chun-Peng , Jeong Joseph H. , Zandi Ebrahim , Roy-Burman Pradip
Published: April 2023

PDF file - 90KB
Supplementary Figures 1 - 9 from Hypoxic Stress Facilitates Acute Activation and Chronic Downregulation of Fanconi Anemia Proteins
Authors: Scanlon Susan E. , Glazer Peter M.
Published: April 2023

PDF file - 1436KB, S1. FANCD2 is ubiquitinated and transcriptionally down-regulated upon treatment with DFX in MCF7 cells. S2. FANCD2 is ubiquitinated and transcriptionally down-regulated upon treatment with DFX in A549 cells. S3. FANCD2 and FANCI ubiquitination does not occur at less severe levels of hypoxia. S4. siRNA depletion of ATR, but not ATM, inhibits FANCD2 and FANCI ubiquitination induced by hypoxia and DFX. S5. FANCD2 and FANCI ubiquitination upon DFX treatment is independent of HIF-1. S6. FANCD2 protein down-regulation by DFX is not due to altered protein stability and transcriptional down-regulation may be related to p130 dephosphorylation and HIF-2. S7. FANCD2 protein level decreases in hypoxia and is restored by HPV16-E7 overexpression. S8. FANCI transcriptional down-regulation upon prolonged exposure to DFX or hypoxia is mitigated by HPV16-E7 overexpression. S9. Increased chromosomal aberrations induced by hypoxia in the absence of FANCD2 are not detected by cytogenetic analysis.
Supplementary Methods from Synergistic Loss of Prostate Cancer Cell Viability by Coinhibition of HDAC and PARP
Authors: Chao Olivia S. , Goodman Oscar B.
Published: April 2023

Supplementary Methods from Synergistic Loss of Prostate Cancer Cell Viability by Coinhibition of HDAC and PARP
Supplementary Figure S3 from Synergistic Loss of Prostate Cancer Cell Viability by Coinhibition of HDAC and PARP
Authors: Chao Olivia S. , Goodman Oscar B.
Published: April 2023

DU145 cells treated with increasing concentration of (a-c) SAHA (0.25-2.0μM) or (d-e) olaparib (5-20μM). BRCA1 and RAD51 nuclear and cytoplasmic protein expression was assessed by western blotting. Fold-change in protein expression over untreated control, normalized to either lamin A/C or α-tubulin for nuclear and cytoplasmic fraction respectively is shown.
Supplementary Methods, Figure Legends, Figures 1 - 3 from Kinesin Family Deregulation Coordinated by Bromodomain Protein ANCCA and Histone Methyltransferase MLL for Breast Cancer Cell Growth, Survival, and Tamoxifen Resistance
Authors: Zou June X. , Duan Zhijian , Wang Junjian , Sokolov Alex , Xu Jianzhen , Chen Christopher Z. , Li Jian Jian , Chen Hong-Wu
Published: April 2023

PDF file - 167K, Supplementary Fig S1. Estrogen induction of kinesins involves assembly and function at promoter of ANCCA, E2F and their associated histone methyltransferase MLL. Supplementary Fig. S2. Kif20A and Kif23 are important for proliferation and survival of tamoxifenresistant breast cancer cells. Supplementary Fig. S3. Expression of ANCCA controlled kinesins is associated with poor outcomes in ER-positive breast tumors.
Supplemental Tables and Figures from STAT1 and NF-κB Inhibitors Diminish Basal Interferon-Stimulated Gene Expression and Improve the Productive Infection of Oncolytic HSV in MPNST Cells
Authors: Jackson Joshua D. , Markert James M. , Li Li , Carroll Steven L. , Cassady Kevin A.
Published: April 2023

Supplemental information which complements the manuscript data. Supplemental Figure 1. Statistical comparison of titers between human and mouse MPNST cell lines. Supplemental Figure 2. Time course of STAT1 phosphorylation in response to oHSV infection. Supplemental Figure 3. STAT1 phosphorylation in response to IFNô€‰´ in human MPNST lines. Supplemental Figure 4. Effect of JAK inhibitor ruxolitinib on M201 spread. Supplemental Figure 5. Endogenous MEK/ERK activation in human MPNST lines.
Supplementary Materials and Methods, Figure Legends from Delineation of a FOXA1/ERα/AGR2 Regulatory Loop That Is Dysregulated in Endocrine Therapy–Resistant Breast Cancer
Authors: Wright Tricia M. , Wardell Suzanne E. , Jasper Jeff S. , Stice James P. , Safi Rachid , Nelson Erik R. , McDonnell Donald P.
Published: April 2023

Supplementary Materials and Methods, Figure Legends from Delineation of a FOXA1/ERα/AGR2 Regulatory Loop That Is Dysregulated in Endocrine Therapy–Resistant Breast Cancer
Supplementary Figure 7 from Delineation of a FOXA1/ERα/AGR2 Regulatory Loop That Is Dysregulated in Endocrine Therapy–Resistant Breast Cancer
Authors: Wright Tricia M. , Wardell Suzanne E. , Jasper Jeff S. , Stice James P. , Safi Rachid , Nelson Erik R. , McDonnell Donald P.
Published: April 2023

Supplementary Figure 7: FOXA1 regulates AGR2 expression in MCF7 GPS ER cells.
Supplementary Figure 6 from Delineation of a FOXA1/ERα/AGR2 Regulatory Loop That Is Dysregulated in Endocrine Therapy–Resistant Breast Cancer
Authors: Wright Tricia M. , Wardell Suzanne E. , Jasper Jeff S. , Stice James P. , Safi Rachid , Nelson Erik R. , McDonnell Donald P.
Published: April 2023

Supplementary Figure 6: ChIP Analysis of AGR2 promoter.
Supplementary Figure 5 from Delineation of a FOXA1/ERα/AGR2 Regulatory Loop That Is Dysregulated in Endocrine Therapy–Resistant Breast Cancer
Authors: Wright Tricia M. , Wardell Suzanne E. , Jasper Jeff S. , Stice James P. , Safi Rachid , Nelson Erik R. , McDonnell Donald P.
Published: April 2023

Supplementary Figure 5: AGR2 regulates ERα expression in both MCF7 and TamR cells.
Supplementary Figure 4 from Hypoxia Regulates Alternative Splicing of HIF and non-HIF Target Genes
Authors: Sena Johnny A. , Wang Liyi , Heasley Lynn E. , Hu Cheng-Jun
Published: April 2023

S4. Activation of endogenous HIF target genes promotes AS of PDK1 minigene.
Supplementary Figure 4 from Delineation of a FOXA1/ERα/AGR2 Regulatory Loop That Is Dysregulated in Endocrine Therapy–Resistant Breast Cancer
Authors: Wright Tricia M. , Wardell Suzanne E. , Jasper Jeff S. , Stice James P. , Safi Rachid , Nelson Erik R. , McDonnell Donald P.
Published: April 2023

Supplementary Figure 4: ERα knockdown in MCF7 and TamR cells.
Supplementary Figure 2 from Delineation of a FOXA1/ERα/AGR2 Regulatory Loop That Is Dysregulated in Endocrine Therapy–Resistant Breast Cancer
Authors: Wright Tricia M. , Wardell Suzanne E. , Jasper Jeff S. , Stice James P. , Safi Rachid , Nelson Erik R. , McDonnell Donald P.
Published: April 2023

Supplementary Figure 2: AGR2 apoptosis analysis in tamoxifen sensitive MCF7 and tamoxifen resistant TamR cells.
Supplementary Figure 2 from Hypoxia Regulates Alternative Splicing of HIF and non-HIF Target Genes
Authors: Sena Johnny A. , Wang Liyi , Heasley Lynn E. , Hu Cheng-Jun
Published: April 2023

S2. Hypoxia regulates AS of additional HIF target genes in Hep3B cells.
Supplemental File 1 from Hypoxia Regulates Alternative Splicing of HIF and non-HIF Target Genes
Authors: Sena Johnny A. , Wang Liyi , Heasley Lynn E. , Hu Cheng-Jun
Published: April 2023

Supplemental File 1: genes that are hypoxia induced or exhibit alternative splicing under hypoxia
Figure S6 from Dissecting LncRNA Roles in Renal Cell Carcinoma Metastasis and Characterizing Genomic Heterogeneity by Single-Cell RNA-seq
Authors: Li Xue , Meng Xianwen , Wei Cong , Zhou Yincong , Chen Hongjun , Huang He , Chen Ming
Published: April 2023

Figure S6. Biological function enrichment analysis for down-regulated and up-regulated CMALs.
Figure S2 from Dissecting LncRNA Roles in Renal Cell Carcinoma Metastasis and Characterizing Genomic Heterogeneity by Single-Cell RNA-seq
Authors: Li Xue , Meng Xianwen , Wei Cong , Zhou Yincong , Chen Hongjun , Huang He , Chen Ming
Published: April 2023

Figure S2. Comparison of the expression of lncRNAs and PCGs in single cells and bulk tissues.
Figure S1 from Dissecting LncRNA Roles in Renal Cell Carcinoma Metastasis and Characterizing Genomic Heterogeneity by Single-Cell RNA-seq
Authors: Li Xue , Meng Xianwen , Wei Cong , Zhou Yincong , Chen Hongjun , Huang He , Chen Ming
Published: April 2023

Figure S1. Expression of lncRNAs and PCGs in single cells and bulk tissues
Supplementary Figure 4 from Taxol Induces Brk-dependent Prosurvival Phenotypes in TNBC Cells through an AhR/GR/HIF–driven Signaling Axis
Authors: Regan Anderson Tarah M. , Ma Shihong , Perez Kerkvliet Carlos , Peng Yan , Helle Taylor M. , Krutilina Raisa I. , Raj Ganesh V. , Cidlowski John A. , Ostrander Julie H. , Schwertfeger Kathryn L. , Seagroves Tiffany N. , Lange Carol A.
Published: April 2023

Western blot of MDA-MB-231 parental or HIF DKO cells demonstrating loss of regulation of PELP1 expression in response to taxol treatment in cells with HIF double-knockout.
Supplementary Figure 3 from Taxol Induces Brk-dependent Prosurvival Phenotypes in TNBC Cells through an AhR/GR/HIF–driven Signaling Axis
Authors: Regan Anderson Tarah M. , Ma Shihong , Perez Kerkvliet Carlos , Peng Yan , Helle Taylor M. , Krutilina Raisa I. , Raj Ganesh V. , Cidlowski John A. , Ostrander Julie H. , Schwertfeger Kathryn L. , Seagroves Tiffany N. , Lange Carol A.
Published: April 2023

Western blot and qPCR data of MDA-MB-231 cells treated for 0-5 days with 5FU, showing increased Brk, HIF-1a, and HIF-2a in response to 5FU.
Figure S2 from p53 Deletion or Hotspot Mutations Enhance mTORC1 Activity by Altering Lysosomal Dynamics of TSC2 and Rheb
Authors: Agarwal Stuti , Bell Catherine M. , Taylor Shirley M. , Moran Richard G.
Published: April 2023

RT-qPCR determination of Rheb mRNA levels isolated from HCT cells wt or null for p53.
Supplementary Figure 1 from Constitutively Active ErbB2 Regulates Cisplatin-Induced Cell Death in Breast Cancer Cells via Pro- and Antiapoptotic Mechanisms
Authors: Sigurðsson Haraldur H. , Olesen Christina W. , Dybboe Rie , Lauritzen Gitte , Pedersen Stine F.
Published: April 2023

Supplementary figure 1: Effects of cisplatin treatment on expression of Î”NErbB2 and NHE1, and subcellular localization of cytochrome c. MCF-7 vector (V) and Î”NErbB2 (Î”N) cells were washed free of tetracycline 48 h before the experiment, and exposed to cisplatin (25 Î¼M) or control conditions for 18 h. Cells were subsequently processed for immunoblotting (A), immunofluorescence analysis (B) or cell surface biotinylation (C-D). A. Î”NErbB2, top panel shows representative immunoblots, lower panel shows Î”NErbB2 protein level relative to that in vector cells under control conditions. B. Subcellular localization of cytochrome c. Nuclei are stained with DAPI. C. Representative immunoblots of NHE1 in total lysate fractions (TLF), intracellular fractions (ITF) and biotinylated fractions (BF) in MCF-7 vector (V) and Î”NErbB2 (Î”N) cells. F. Top panel shows representative immunoblots of NHE1 in TLF and BF in native MCF-7 cells, lower panel shows NHE1 protein level relative to that in native MCF-7 cells under control conditions. Data are representative of 3-5 independent experiments for each condition. Western blot data are shown as means with SEM error bars. * indicates comparison of the value in cisplatin treated to that in the respective cell line control, # indicate comparison of values in vector- and Î”NErbB2 cell lines under same treatment. */#, **/##, ***/### and ****/#### indicate p < 0.05, p < 0.01, p < 0.001 and p < 0.0001, respectively (C, one-way ANOVA with Bonferroni post-test) (A, two-way ANOVA with Bonferroni post-test).
Suppl-Figure 2 from Pancreatic Neuroendocrine Tumors and EMT Behavior Are Driven by the CSC Marker DCLK1
Authors: Ikezono Yu , Koga Hironori , Akiba Jun , Abe Mitsuhiko , Yoshida Takafumi , Wada Fumitaka , Nakamura Toru , Iwamoto Hideki , Masuda Atsutaka , Sakaue Takahiko , Yano Hirohisa , Tsuruta Osamu , Torimura Takuji
Published: April 2023

Comparison of DCLK1 immunostaining scores between primary PNETs and metastatic sites (lymph nodes) in four cases. n.s., not significant.
Table S3 from EGFR Downregulation after Anti-EGFR Therapy Predicts the Antitumor Effect in Colorectal Cancer
Authors: Okada Yasuyuki , Kimura Tetsuo , Nakagawa Tadahiko , Okamoto Koichi , Fukuya Akira , Goji Takahiro , Fujimoto Shota , Sogabe Masahiro , Miyamoto Hiroshi , Muguruma Naoki , Tsuji Yasushi , Okahisa Toshiya , Takayama Tetsuji
Published: April 2023

The relationship between EGFR downregulation and early tumor shrinkage
Figure S1 from Differential Expression of OATP1B3 Mediates Unconjugated Testosterone Influx
Authors: Sissung Tristan M. , Ley Ariel M. , Strope Jonathan D. , McCrea Edel M. , Beedie Shaunna , Peer Cody J. , Shukla Suneet , van Velkinburgh Jennifer , Reece Kelie , Troutman Sarah , Campbell Tessa , Fernandez Elena , Huang Phoebe , Smith Jordan , Thakkar Nilay , Venzon David J. , Brenner Stefan , Lee Wooin , Merino Maria , Luo Ji , Jager Walter , Price Douglas K. , Chau Cindy H. , Figg William D.
Published: April 2023

S1. Expression of (A) doxycycline-inducible GFP-linked OATP1B3 was confirmed in the membrane and not cytosolic fractions of PC3 cells using a GFP-specific antibody Abcam goat antibody (#ab6673).
Table S1 from p53 Activity Dominates That of p73 upon Mdm4 Loss in Development and Tumorigenesis
Authors: Tashakori Mehrnoosh , Zhang Yun , Xiong Shunbin , You M. James , Lozano Guillermina
Published: April 2023

p73 is haploinsufficient to rescue the Mdm4âˆ†2/âˆ†2 p53+/- phenotype.
Supplementary Figure Legends from Metabolic Reprogramming by Folate Restriction Leads to a Less Aggressive Cancer Phenotype
Authors: Ashkavand Zahra , O'Flanagan Ciara , Hennig Mirko , Du Xiuxia , Hursting Stephen D. , Krupenko Sergey A.
Published: April 2023

Supplementary Fig. 1. Levels of folate enzymes in E-Wn, M-Wnt or metM-Wntliver cells measured by a dot blot assay. Supplementary Fig. 2. Folate restriction inhibits cellular migration.
Supplementary Methods, Figure Legends from Beneficial Effects of RAF Inhibitor in Mutant BRAF Splice Variant–Expressing Melanoma
Authors: Hartsough Edward J. , Basile Kevin J. , Aplin Andrew E.
Published: April 2023

PDF file - 75K
Supplemental Legends from Hypoxia Selectively Enhances Integrin α5β1 Receptor Expression in Breast Cancer to Promote Metastasis
Authors: Ju Julia A. , Godet Inês , Ye I Chae , Byun Jungmin , Jayatilaka Hasini , Lee Sun Joo , Xiang Lisha , Samanta Debangshu , Lee Meng Horng , Wu Pei-Hsun , Wirtz Denis , Semenza Gregg L. , Gilkes Daniele M.
Published: April 2023

Supplemental Legends
Supplementary Figures 1 - 4 from Beneficial Effects of RAF Inhibitor in Mutant BRAF Splice Variant–Expressing Melanoma
Authors: Hartsough Edward J. , Basile Kevin J. , Aplin Andrew E.
Published: April 2023

PDF file - 369K, Supplemental Figure 1. PRT #3 and PRT #4 signaling analysis. Supplemental Figure 2. Invasiveness of PRT #3 and PRT #4. Supplemental Figure 3. PRT #3 and PRT #4 display an elongated, spindle-like appearance after RAF inhibitor withdrawal. Supplemental Figure 4. Additive effect of PLX4720 and GSK'212 suppresses cell proliferation and ERK1/2 phosphorylation.
Figure S4 from Hypoxia Selectively Enhances Integrin α5β1 Receptor Expression in Breast Cancer to Promote Metastasis
Authors: Ju Julia A. , Godet Inês , Ye I Chae , Byun Jungmin , Jayatilaka Hasini , Lee Sun Joo , Xiang Lisha , Samanta Debangshu , Lee Meng Horng , Wu Pei-Hsun , Wirtz Denis , Semenza Gregg L. , Gilkes Daniele M.
Published: April 2023

Characterization of fibronectin-collagen gels.
Supplementary Figure 1 from Differential Expression of Stress and Immune Response Pathway Transcripts and miRNAs in Normal Human Endothelial Cells Subjected to Fractionated or Single-Dose Radiation
Authors: Palayoor Sanjeewani T. , John-Aryankalayil Molykutty , Makinde Adeola Y. , Falduto Michael T. , Magnuson Scott R. , Coleman C. Norman
Published: April 2023

PDF - 1331K, Western Blot Analysis.
Supplemental Data from β-Arrestin-2 Counters CXCR7-Mediated EGFR Transactivation and Proliferation
Authors: Kallifatidis Georgios , Munoz Daniel , Singh Rajendra Kumar , Salazar Nicole , Hoy James J. , Lokeshwar Bal L.
Published: April 2023

Supplemental Data
Blaesius_Manuscript_MCR_Aug26_2016_Supplemental tables and figures from Immunophenotyping and Transcriptomic Outcomes in PDX-Derived TNBC Tissue
Authors: Snowden Eileen , Porter Warren , Hahn Friedrich , Ferguson Mitchell , Tong Frances , Parker Joel S. , Middlebrook Aaron , Ghanekar Smita , Dillmore W. Shannon , Blaesius Rainer
Published: April 2023

Cell sorting strategy, Pathway maps for differentially expressed genes, Differentially expressed surface markers, AXL gene set used in analysis. Figure S1: Staining and gating strategy of flow cytometry analysis. Figure S2: Sorting strategy to isolate CD184hi and CD184lo subpopulations. Figure S3: Cell Cycle Pathway Regulation Figure S4: DNA Replication Pathway Regulation Figure S5: G1/S Transition Pathway Regulation Figure S6: Angiogenesis Pathway Regulation Table S1: List of CD surface marker genes differentially regulated (CD184lo/CD184hi) at FDR<0.05 and xfold >4. Table S2: AXL gene set and Fold - change in CD184lo vs. CD184hi population. Table S3: Full RNASeq dataset in Excel format.
Figure S3 from MEK and TAK1 Regulate Apoptosis in Colon Cancer Cells with KRAS-Dependent Activation of Proinflammatory Signaling
Authors: McNew Kelsey L. , Whipple William J. , Mehta Anita K. , Grant Trevor J. , Ray Leah , Kenny Connor , Singh Anurag
Published: April 2023

Synthetic lethality of TAK1 depletion in KRAS transformed cells.
Supplemental Figure 7 from Tumor Suppressor Activity of Selenbp1, a Direct Nkx2-1 Target, in Lung Adenocarcinoma
Authors: Caswell Deborah R. , Chuang Chen-Hua , Ma Rosanna K. , Winters Ian P. , Snyder Eric L. , Winslow Monte M.
Published: April 2023

Nkx2-1 is stabilized in the presence of Selenbp1.
Supplemental Figure 5 from Tumor Suppressor Activity of Selenbp1, a Direct Nkx2-1 Target, in Lung Adenocarcinoma
Authors: Caswell Deborah R. , Chuang Chen-Hua , Ma Rosanna K. , Winters Ian P. , Snyder Eric L. , Winslow Monte M.
Published: April 2023

Nkx2-1 is required and sufficient for Selenbp1 expression, and Nkx2-1 and its target Selenbp1 inhibit migration independent of their effect on proliferation.
Supplemental Figure 4 from Tumor Suppressor Activity of Selenbp1, a Direct Nkx2-1 Target, in Lung Adenocarcinoma
Authors: Caswell Deborah R. , Chuang Chen-Hua , Ma Rosanna K. , Winters Ian P. , Snyder Eric L. , Winslow Monte M.
Published: April 2023

Selenbp1 and Nkx2-1 expression correlates in tumors from mouse lung adenocarcinoma models
Supplementary Data File 2 from GR and ER Coactivation Alters the Expression of Differentiation Genes and Associates with Improved ER+ Breast Cancer Outcome
Authors: West Diana C. , Pan Deng , Tonsing-Carter Eva Y. , Hernandez Kyle M. , Pierce Charles F. , Styke Sarah C. , Bowie Kathleen R. , Garcia Tzintzuni I. , Kocherginsky Masha , Conzen Suzanne D.
Published: April 2023

Analyzed gene expression data for GR- and ER-bound genes
Supplemental Figures and Legends 1-6 from A Systematic Analysis of Negative Growth Control Implicates the DREAM Complex in Cancer Cell Dormancy
Authors: MacDonald James , Ramos-Valdes Yudith , Perampalam Pirunthan , Litovchick Larissa , DiMattia Gabriel E. , Dick Frederick A.
Published: April 2023

S1. Cumulative viability score of siRNA knockdowns. S2. Spheroid morphology of control and gene knock down cells. S3. Reattachment of control and gene knockdown spheroid cells after 72 hours. S4. Relative protein expression levels of Dyrk1A and Dyrk1B in cell lines used in this study. S5. Expression and function of CIP/KIPproteins in non-adherent culture. S6. Flow cytometry analysis of p130 depleted cells.
Supplemental Data from Synthetic Lethality in PTEN-Mutant Prostate Cancer Is Induced by Combinatorial PI3K/Akt and BCL-XL Inhibition
Authors: Ren Wenying , Joshi Raghav , Mathew Paul
Published: April 2023

Supplemental Fig 1, Fig 2, Table 1, Table 2 with Supplemental Methods
Figure S1 from EGFR Mutations Compromise Hypoxia-Associated Radiation Resistance through Impaired Replication Fork–Associated DNA Damage Repair
Authors: Saki Mohammad , Makino Haruhiko , Javvadi Prashanthi , Tomimatsu Nozomi , Ding Liang-Hao , Clark Jennifer E. , Gavin Elaine , Takeda Kenichi , Andrews Joel , Saha Debabrata , Story Michael D. , Burma Sandeep , Nirodi Chaitanya S.
Published: April 2023

MT-EGFR expression reduces survival in NSCLCs and HBEC cells.
Data Supplement from Discovery-Based Protein Expression Profiling Identifies Distinct Subgroups and Pathways in Leiomyosarcomas
Authors: Kirik Ufuk , Hansson Karin , Krogh Morten , Jönsson Mats , Nilbert Mef , James Peter , Carneiro Ana
Published: April 2023

Supplementary Figure Legends
Supplemental Figure Legend from TNF Signaling through RIP1 Kinase Enhances SN38-Induced Death in Colon Adenocarcinoma
Authors: Cabal-Hierro Lucia , O'Dwyer Peter J.
Published: April 2023

Supplemental Figure Legend
Supplemental Table 1 from Proteomic Characterization of Head and Neck Cancer Patient–Derived Xenografts
Authors: Li Hua , Wheeler Sarah , Park Yongseok , Ju Zhenlin , Thomas Sufi M. , Fichera Michele , Egloff Ann M. , Lui Vivian W. , Duvvuri Umamaheswar , Bauman Julie E. , Mills Gordon B. , Grandis Jennifer R.
Published: April 2023

Proteins expressed at similar levels in both primary HNSCC and PDXs
Supplemental Figure 2a from Proteomic Characterization of Head and Neck Cancer Patient–Derived Xenografts
Authors: Li Hua , Wheeler Sarah , Park Yongseok , Ju Zhenlin , Thomas Sufi M. , Fichera Michele , Egloff Ann M. , Lui Vivian W. , Duvvuri Umamaheswar , Bauman Julie E. , Mills Gordon B. , Grandis Jennifer R.
Published: April 2023

Primary HNSCC and PDX jitter plots for range data visualization. 173 primary HNSCC and 12 PDXs are plotted in separate columns by protein. A) 76 proteins expressed at similar levels in both primary HNSCC and PDX.
Supplemental Figure 1b from Proteomic Characterization of Head and Neck Cancer Patient–Derived Xenografts
Authors: Li Hua , Wheeler Sarah , Park Yongseok , Ju Zhenlin , Thomas Sufi M. , Fichera Michele , Egloff Ann M. , Lui Vivian W. , Duvvuri Umamaheswar , Bauman Julie E. , Mills Gordon B. , Grandis Jennifer R.
Published: April 2023

HNSCC Protein expression is not associated with patient age or tumor site. 55 deidentified primary HNSCC with TCPA data were analyzed by unsupervised clustering. B) Tumor site is represented above the heatmap for each tumor.
Supplemental Figure 1a from Proteomic Characterization of Head and Neck Cancer Patient–Derived Xenografts
Authors: Li Hua , Wheeler Sarah , Park Yongseok , Ju Zhenlin , Thomas Sufi M. , Fichera Michele , Egloff Ann M. , Lui Vivian W. , Duvvuri Umamaheswar , Bauman Julie E. , Mills Gordon B. , Grandis Jennifer R.
Published: April 2023

HNSCC Protein expression is not associated with patient age or tumor site. 55 deidentified primary HNSCC with TCPA data were analyzed by unsupervised clustering. A) Age categories (by years of age) are represented above the heatmap for each tumor.
Supplementary figure legends from STAT3 and HIF1α Signaling Drives Oncogenic Cellular Phenotypes in Malignant Peripheral Nerve Sheath Tumors
Authors: Rad Ellie , Dodd Kayleigh , Thomas Laura , Upadhyaya Meena , Tee Andrew
Published: April 2023

Supplementary figure legends
Supplementary Figure 2 from STAT3 and HIF1α Signaling Drives Oncogenic Cellular Phenotypes in Malignant Peripheral Nerve Sheath Tumors
Authors: Rad Ellie , Dodd Kayleigh , Thomas Laura , Upadhyaya Meena , Tee Andrew
Published: April 2023

Supplementary Figure 2. Cucurbitacin-I greatly altered the morphology of the S1844.1 and S1507.2 cell lines. Starved S1844.1 and S1507.2 cells were subjected to wound healing assay. Cells were pre-treated with 5 nM Cucurbitacin-I, for 30 min prior to 30 min 20 ng/ml HGF stimulation. Pictures were taking at 0 and 18 h. n=3.
Supplementary Figure 1 from STAT3 and HIF1α Signaling Drives Oncogenic Cellular Phenotypes in Malignant Peripheral Nerve Sheath Tumors
Authors: Rad Ellie , Dodd Kayleigh , Thomas Laura , Upadhyaya Meena , Tee Andrew
Published: April 2023

Supplementary Figure 1. ST8814 and S462 cells have markedly different STAT3 signalling profiles after HGF stimulation. Starved ST8814 and S462 cells were stimulated with 20 ng/ml HGF for the indicated time (0 h, 1 h, 2 h, and 3 h). Total and phosphorylated STAT3 (Tyr 705) was determined from protein lysates. β-actin serves as a loading control.
Supplementary Figure 4 from Identification of mTORC2 as a Necessary Component of HRG/ErbB2-Dependent Cellular Transformation
Authors: Lin Miao-chong J. , Rojas Katherine S. , Cerione Richard A. , Wilson Kristin F.
Published: April 2023

PDF file - 42K, Attenuation of HRG-stimulated AKT phosphorylation by the knock-down of Rictor can be rescued by the expression of an shRNA-insensitive Rictor construct. SKBR3 cells were infected either with control or Rictor shRNA-containing virus twice, one day apart, and selected with 2 microg/mL puromycin for 48 h. Cells were then subjected to transfection either with mock (no DNA) or 5 microg of Rictor plasmid for 3 hours, followed by serum-starvation for 48 h. On the day of the harvest, cells were treated with or without 1 nM HRG. Whole cell lysates were collected and subjected to Western blotting probing for Rictor, phospho-AKT (T308), phospho-AKT (S473), and total AKT.
Tissue Microarray Analysis of Cdh1 Scoring in Tumor Lymph Node Samples from PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27
Authors: Link Laura A. , Howley Breege V. , Hussey George S. , Howe Philip H.
Published: April 2023

S4. Scoring of tissue microarray tumor lymph node samples for Cdh1 staining and patient recurrence status.
Supplementary Figure 2 from Identification of mTORC2 as a Necessary Component of HRG/ErbB2-Dependent Cellular Transformation
Authors: Lin Miao-chong J. , Rojas Katherine S. , Cerione Richard A. , Wilson Kristin F.
Published: April 2023

PDF file - 201K, HRGalpha, as well as HRGbeta, is capable of stimulating the ability of SKBR3 cells to form colonies and activate components of the mTORC1 signaling pathway. A, SKBR3 cells were seeded in 0.3% agarose-containing complete medium with the addition of 1 nM HRGalpha or 1 nM HRGbeta. Cells were fed every three days with the growth factor-containing medium and colonies were counted on day 13. The experiment was performed in triplicate and the results were averaged and graphed. B, SKBR3 cells were serum-starved for 40-48 h, and were then challenged with HRGalpha for the indicated times. Cell lysates were then collected and analyzed by Western blotting to determine relative levels of phospho-mTOR (S2448), phospho-TSC2 (T1462), phospho-AKT (T308), phospho-AKT (S473), phospho-ribosomal S6 (S235/236), actin, as well as for the total levels of the respective proteins.
Supplementary Figure 1 from Identification of mTORC2 as a Necessary Component of HRG/ErbB2-Dependent Cellular Transformation
Authors: Lin Miao-chong J. , Rojas Katherine S. , Cerione Richard A. , Wilson Kristin F.
Published: April 2023

PDF file - 136K, HRG-dependent stimulation of mTORC1-related signaling activities in SKBR3 cells is blocked by pre-treatment with the ErbB2-specific kinase inhibitor, CP-724,714. SKBR3 cells were serum-starved followed by treatment with or without 5 microM CP-724,714 for 30 minutes prior to stimulation with 1 nM HRG for an additional 30 minutes. Cells were collected and lysed, and the lysates were analyzed by Western blotting using specific antibodies to probe for phospho-ErbB2 (Y1248), phospho-mTOR (S2448), phospho-TSC2 (T1462), phospho-AKT (T308), phospho-AKT (S473), phospho-ribosomal S6 (S235/236), the corresponding pan-specific antibodies, or actin.
Supplementary Methods from Functional Genomics Approach Identifies Novel Signaling Regulators of TGFα Ectodomain Shedding
Authors: Wilson Jennifer L. , Kefaloyianni Eirini , Stopfer Lauren , Harrison Christina , Sabbisetti Venkata S. , Fraenkel Ernest , Lauffenburger Douglas A. , Herrlich Andreas
Published: April 2023

Network optimization methods.
Supplementary Figure S3 from miR-211-5p Suppresses Metastatic Behavior by Targeting SNAI1 in Renal Cancer
Authors: Wang Kefeng , Jin Wei , Jin Peng , Fei Xiang , Wang Xia , Chen Xiaonan
Published: April 2023

Supplementary Figure S3
Supplementary Figures from Functional Genomics Approach Identifies Novel Signaling Regulators of TGFα Ectodomain Shedding
Authors: Wilson Jennifer L. , Kefaloyianni Eirini , Stopfer Lauren , Harrison Christina , Sabbisetti Venkata S. , Fraenkel Ernest , Lauffenburger Douglas A. , Herrlich Andreas
Published: April 2023

Figures for network optimization, shEnrich scoring results for all family sizes, the unclustered network, and additional network validation experiments.
Supplementary Figure S2 from miR-211-5p Suppresses Metastatic Behavior by Targeting SNAI1 in Renal Cancer
Authors: Wang Kefeng , Jin Wei , Jin Peng , Fei Xiang , Wang Xia , Chen Xiaonan
Published: April 2023

Supplementary Figure S2