bluuebunny/crossref_metadata_2025 huggingface dataset:
Function of the NAC1 Gene from Fraxinus mandshurica in Cold Resistance and Growth Promotion in Tobacco
Authors: Cao Yang , He Liming , Lu Shengdian , Wang Yuling , Zhang Chenxi , Zhan Yaguang
Published: August 2024

To elucidate the function of the cold-resistance regulatory gene FmNAC1 from Fraxinus mandshurica Rupr., this study identified the role that overexpression of the FmNAC1 gene plays in tobacco growth and cold-stress regulation. The cloned FmNAC1 gene from F. mandshurica is 891 bp in length and encodes 296 amino acids. Our subcellular localization analysis confirmed that FmNAC1 is primarily located in the nucleus and functions as a transcription factor. FmNAC1 is responsive to cold and NaCl stress, as well as to the induction of IAA, GA, and ABA hormone signals. To further elucidate its function in cold resistance, four transgenic tobacco lines expressing FmNAC1 (FmNAC1-OE) were generated through tissue culture after the Agrobacterium-mediated transformation of wild-type (WT) Nicotiana tabacum L. These FmNAC1-OE plants exhibited accelerated growth after transplantation. When exposed to low-temperature conditions at −5 °C for 24 h, the rates of wilting and yellowing of the FmNAC1-OE plants were significantly lower than those of the WT tobacco plants. Additionally, the membrane integrity, osmotic regulation, and reactive oxygen species (ROS)-scavenging abilities of the FmNAC1-OE tobacco lines were better than those of the WT plants, indicating the potential of the FmNAC1 gene to improve plant cold resistance. The gene expression results further revealed that the FmNAC1 transcription factor exhibits regulatory interactions with growth-related genes such as IAA and AUX1; cold-resistance-related genes such as ICE, DREB, and CBF1; and genes involved in the clearance of reactive oxygen species (ROS), such as CAT and SOD. All of this evidence shows that the FmNAC1 transcription factor from F. mandshurica plays a key role in contributing to the enhancement of growth, cold resistance, and ROS clearance in transgenic tobacco plants.
Easement Reform and Employment Transfer of Forest Farmers: Evidence from China’s National Parks
Authors: Liu Qiang , Jin Xinyu , Li Lanying , Xu Qianqian
Published: August 2024

The easement reform of collective forest land (ERCFL) is an important part of national park system construction, which plays an important role in promoting the employment transfer of forest farmers. On the basis of survey data from forest farmers in Qianjiangyuan–Baishanzu National Park in Zhejiang Province, this paper uses the propensity score matching-difference in differences model to empirically analyze the impact of ERCFL on the transfer employment behavior and employment quality of forest farmers to provide experience for promoting the employment transfer of forest farmers and improving the ERCFL in national parks. This research shows the following: (1) The ERCFL in national parks has significantly promoted the employment transfer and improved the employment quality of forest farmers. This effect remains significant when controlling for possible endogeneity issues. (2) Three mechanisms of the ERCFL affect forest farmers’ transfer of employment, namely improving non-agricultural employment skills, expanding the scope of non-agricultural employment, and increasing non-agricultural employment opportunities. (3) Significant gender, age, and education differences exist in terms of the impact of the ERCFL on the employment transfer of forest farmers. The impact on men, middle-aged and elderly groups, and low-education groups is more significant. Finally, this paper proposed forward countermeasures and suggestions to promote the employment transfer of forest farmers.
Photosynthesis and Latex Burst Characteristics in Different Varieties of Rubber Trees (Hevea brasiliensis) under Chilling Stress, Combing Bark Tensile Property and Chemical Component Analysis
Authors: Cheng Linlin , Jiang Huichuan , Xie Guishui , Wang Jikun , Peng Wentao , Zhou Lijun , An Feng
Published: August 2024

Rubber trees (Hevea brasiliensis) serve as the primary source of natural rubber. Their native habitat is characterized by warm and humid conditions, so they are particularly sensitive to low temperatures. Under such stress, latex burst can cause severe damage to rubber trees, which is due to the uniqueness of their economically productive parts. In order to establish a correlation between young and mature rubber trees and provide a novel prospective for investigating the mechanisms of latex burst and chilling resistance in rubber trees, the chlorophyll contents, photosynthesis, and chlorophyll fluorescence parameters in four varieties of one-year-old rubber tree seedlings were analyzed under artificially simulated chilling stress. The latex burst characteristics were subsequently recorded. A comprehensive statistical analysis of the chilling-resistance rank was conducted using the membership function method and the combination weighting method. Meanwhile, chemical compositions and tensile properties of barks from two-year-old twigs of mature rubber trees were ascertained. A correlation analysis between chilling resistance, chemical compositions, and tensile properties was performed to identify any interrelationships among them. The results showed that the number and the total area of latex-burst positions in variety Reken628 seedlings were greater than those in other varieties, and the lowest number and total area of latex-burst positions were observed in variety RRIM600 and variety PR107, respectively. With the exception of variety GT1, nectar secretion was noted in all other varieties of rubber tree seedlings under chilling stress. The chilling resistance of the four varieties decreased in the following order: variety GT1 > variety RRIM600 > variety PR107 > variety Reken628. The chilling resistance was strongly (p < 0.001) negatively correlated with cellulose content and acid-insoluble lignin content, respectively. The total area of latex burst was significantly (p < 0.001) and positively correlated with holocellulose content and maximum load, respectively. Furthermore, this study also provides new insights into the mechanism of nectar secretion induced by low temperatures and its association with the chilling resistance of rubber trees.
What Are the Variation Patterns of Vegetation and Its Influencing Factors in China from 2000–2020 from the Partition Perspective?
Authors: Guo Bing , Xu Mei , Zhang Rui , Luo Wei , Yang Jicun
Published: August 2024

China’s vegetation ecosystem has undergone profound changes, and there is an urgent need to explore the mechanisms behind vegetation changes in different ecological sub-regions and historical periods across China. Based on NDVI (normalized difference vegetation index) data, this study analyzed the spatial and temporal evolution patterns and driving mechanisms of six ecological sub-regions in China. The results showed that: (1) over the past 20 years, the vegetation coverage in mainland China showed a decreasing trend from east to west. (2) Over the past 20 years, the vegetation coverage of the six ecological sub-regions showed an increasing trend, with the highest increase in Central Southern China (0.0039) and the lowest increase in East China (0.002). (3) The gravity center of Northeast China showed a trend of migration to the northwest. The gravity center of North China, East China, and Central South China showed a trend of migration to the southwest, while that of Northwest and Southwest China showed a trend of migration to the southeast. (4) During the period from 2000 to 2020, vegetation cover levels showed an upward trend. (5) The lag time of vegetation types in different regions was different. (6) Precipitation was the dominant influencing factor in the evolution of vegetation in Northeast China, North China, Northwest China, and Southwest China. The dominant influencing factors of vegetation evolution in East China were land use and GDP (gross domestic product), while the dominant influencing factors of vegetation evolution in Central South China were precipitation and land use.
Fabrication of Silica–Titanium Composite Film on Wood Surface and Optimization of Its Structure and Properties
Authors: Liu Zhigao , Li Qianying , Cheng Si , Wei Penglian , Fu Yunlin
Published: August 2024

In this thesis, wood loaded with a silica–titanium (Si-Ti) composite film was prepared using the sol–gel method in order to achieve improved wood with high hydrophobicity and photocatalytic activity under visible light. The factors affecting the structure and properties of the composite film, as well as the optimization process, were discussed. Infrared analysis revealed that the vibrational intensity of Si-O-Si, Ti-O-Ti, and Ti-O-Si telescopic vibration peaks increased with an increase in vinyltriethoxysilane (VETS). Additionally, the number of Ti-O-Ti telescopic vibration peaks also increased with an increase in VETS. Furthermore, the intensity of -NO3, Si-O-Si, and Ti-O-Ti telescopic vibrational peaks was enhanced with a higher dosage of nitric acid. Conversely, the intensity of -OH telescopic vibrational peaks decreased with an increase in drying temperature. XRD analysis showed that nitric acid could promote the transformation of TiO2 from amorphous to anatase, while SiO2 would reduce the grain size of anatase TiO2 and promote the growth of rutile TiO2. Additionally, wood surfaces loaded with Si-Ti composite film changed from hydrophilic to hydrophobic, with significant differences observed between different levels of each factor. The photocatalytic activity of surface-loaded Si-Ti composite films on wood was most affected by the amount of nitric acid, which influenced crystallinity of TiO2 and thus impacted the photocatalytic activity. Furthermore, changes in VTES dosage not only affected the crystalline phase of TiO2 and the grain size of Si-Ti composite film but also influenced the crystallinity of TiO2 through generating SiO2. Finally, based on optimal preparation process (titanium–alcohol ratio of 1:5, titanium–silicon ratio of 1:0.2, titanium–acid ratio of 1:0.5, and drying temperature of 100 °C), wood surfaces loaded with Si-Ti composite film achieved a contact angle up to 125.9° and exhibited a decolorization rate for rhodamine B under UV light reaching 94% within 180 min.
Does Forest Contemplation Provide Greater Psychological Benefits than Passive Exposure to the Urban Forest? A Pilot Study
Authors: Janeczko Emilia , Woźnicka Małgorzata , Śmietańska Katarzyna , Wiśniewska Anna , Korcz Natalia , Kobyłka Agata
Published: August 2024

Human contact with the natural environment effectively counteracts negative symptoms of stress and has many positive psychological effects. For this reason, forests within city limits are increasingly seen as part of public health promotion. Being in an urban forest can take many forms, but there is still little known about whether being in a forest alone, without engaging one’s senses, is as effective for human renewal as an experience that involves greater, more conscious activation of the senses of sight, hearing, smell. A study measuring the effect of the forest (spring aspect) on human mental relaxation during passive exposure was conducted on a sample of 19 Polish young adults. The experiment consisted of two series. In the first, participants read an academic textbook in a tree stand for 20 min, while in the next session—conducted at the same location—they contemplated the landscape. Before the experiment and after each of the two series of tests, four psychological questionnaires were administered: the Profile of Mood State (POMS), Positive and Negative Affect Schedule (PANAS), the Restorative Outcome Scale (ROS) and the Subjective Vitality Scale (SVS). Analyses showed that being in a forest environment positively influenced the subjects’ mental relaxation, while the level of benefit from the activation of the senses, especially vision (looking at the forest) was significantly higher compared to the passive activity of reading a text. The research indicates that forest bathing is the best form of relaxation that can be obtained through contact with the forest.
Bioprocess Optimization by Taguchi Design and Response Surface Analysis for Obtaining Active Yeast Used in Vinification
Authors: Dumitrache Corina , Ghica Mihaela Violeta , Frîncu Mihai , Bărbulescu Iuliana Diana , Begea Mihaela , Diguță Camelia Filofteia , Baniță Cornel , Cotea Valeriu V. , Israel-Roming Florentina , Teodorescu Răzvan Ionuț
Published: August 2024

This study presents the behavior of the identified yeast strain S. cerevisiae, isolated from Busuioacă de Bohotin grapes from the Pietroasa winery, during the process of fermentation to obtain dry active yeast biomass for the winemaking process. In this respect, we promoted an optimization strategy for obtaining active dry yeast biomass. The cultivation conditions for micropilot fermentation (temperature, pH, carbon source, and nitrogen source) were selected and designed according to a Taguchi design with four factors and three levels. Reproducibility testing was conducted under specific fermentation parameters: temperature (32 °C), pH (4.5), carbon source (12%), and nitrogen source (0.7%). Following the optimization process, two combinations of cultivation parameters were selected, and one of them, based on the results, was selected for further analysis. Quantitative data were obtained, showing dry yeast biomass (DCW) at 1.39 g/100 mL and protein content at 45.57%. The active yeast was then used in the winemaking process for Tămâioasă Românească and Busuioacă de Bohotin varieties at Pietroasa winery for validation. This optimization aims to facilitate easy and rapid production of fresh wine yeast tailored to the local winemaking practices of Pietroasa winery, with real application potential in other viticultural areas, aligning with the terroir concept.
Blockchain Technology and Its Potential to Benefit Public Services Provision: A Short Survey
Authors: Piccardo Giorgio , Conti Lorenzo , Martino Alessio
Published: August 2024

In the last few years, blockchain has emerged as a cutting-edge technology whose main advantages are transparency, traceability, immutability, enhanced efficiency, and trust, thanks to its decentralized nature. Although many people still identify blockchain with cryptocurrencies and the financial sector, it has many prospective applications beyond digital currency that can serve as use cases for which traditional infrastructures have become obsolete. Governments have started exploring its potential application to public services provision, as confirmed by the increasing number of adoption initiatives, projects, and tests. As the current public administration is often perceived as slow, bureaucratic, lacking transparency, and failing to involve citizens in decision-making processes, blockchain can establish itself as a tool that enables a process of disintermediation, which can revolutionize the way in which public services are managed and provided. In this paper, we will provide a survey of the main application areas which are likely to benefit from blockchain implementation, together with examples of practical implementations carried out by both state and local governments. Later, we will discuss the main challenges that may prevent its widespread adoption, such as government expenditure, technological maturity, and lack of public awareness. Finally, we will wrap up by providing indications on future areas of research for blockchain-based technologies.
Early Ransomware Detection with Deep Learning Models
Authors: Davidian Matan , Kiperberg Michael , Vanetik Natalia
Published: August 2024

Ransomware is a growing-in-popularity type of malware that restricts access to the victim’s system or data until a ransom is paid. Traditional detection methods rely on analyzing the malware’s content, but these methods are ineffective against unknown or zero-day malware. Therefore, zero-day malware detection typically involves observing the malware’s behavior, specifically the sequence of application programming interface (API) calls it makes, such as reading and writing files or enumerating directories. While previous studies have used machine learning (ML) techniques to classify API call sequences, they have only considered the API call name. This paper systematically compares various subsets of API call features, different ML techniques, and context-window sizes to identify the optimal ransomware classifier. Our findings indicate that a context-window size of 7 is ideal, and the most effective ML techniques are CNN and LSTM. Additionally, augmenting the API call name with the operation result significantly enhances the classifier’s precision. Performance analysis suggests that this classifier can be effectively applied in real-time scenarios.
Mitogenome, Poly(A) Mitotranscriptome, and Molecular Phylogeny of Rasbora rasbora (Family Danionidae; Subfamily Rasborinae)
Authors: Profant Stanislava Wolf , Jørgensen Tor Erik , Austad Eirik , Babiak Igor , Johansen Steinar Daae
Published: August 2024

The subfamily Rasborinae is a species-rich group of freshwater fish related to zebrafish; however, its taxonomy remains unclear. We present the complete mitogenome and corresponding polyadenylated mitotranscriptome of Rasbora rasbora (Hamilton 1822) based on long-read and high-coverage Oxford Nanopore Technology sequencing. The mitogenome size, gene content, and gene organization correspond to the typical vertebrate composition, and the mitogenome generates 10 polyadenylated mRNAs. Two alternative polyadenylation sites of ND5 mRNA were detected, one with a 596 nt 3′untranslated region corresponding to the antisense ND6 gene. Polyadenylation also generates seven of the mRNA UAA stop codons. Complete mitogenome sequences, excluding the control region, were carefully aligned for RNA-coding and protein-coding features using 54 available species of the subfamily Rasborinae. The phylogenetic analyses based on maximum likelihood, Bayesian inference, and neighbor-joining tree building methods confirm the transfer of R. rasbora into the Sumatrana species group. The overall phylogeny of the subfamily Rasborinae supports with high confidence some previously observed changes within this subfamily, as well as contradicts some conclusions set by previous studies.
Assessment and Spatio-Temporal Evolution of Marine Fisheries’ Carbon Sink Capacity in China’s Three Marine Economic Circles
Authors: Jin Yue , Ma Jintao , Li Cheng , Hu Qiuguang
Published: August 2024

With the increasing pressure of resource decline and environmental pollution faced by the green transformation of marine fisheries, marine fishery carbon sinks are an increasingly close link to national strategic interests and economic lifelines. It is, therefore, necessary to explore paths for the enhancement and development of the carbon sink capacity of marine fisheries. Based on the economic data of marine fisheries from 2002 to 2021, this paper measures the capacity and characteristics of marine fishery carbon sinks in provincial areas, applies the kernel density estimation method to depict the dynamic evolution of the distribution of absolute differences in marine fishery carbon sinks, and at the same time, identifies the factors influencing the spatial imbalance in the development of marine fishery carbon sinks as well as the spatial spillover effects. The results show the following: (1) From the total amount, the fishery carbon sink capacity of China’s three marine economic circles has fluctuated and increased over the past 20 years, with obvious differences among different circles, and the marine fishery carbon sink capacity of the eastern, northern, and southern marine economic circles has risen sequentially. This trend corresponds to the economic disparities among the three circles, demonstrating a transition from economic to industrial driving effects. (2) From the spatial point of view, the fishery carbon sink capacity shows a trend of increasing year by year, concentrating in the circles, and overflowing between the circles. (3) The results of a partial derivative test further show that among the main influencing factors of marine fishery carbon sinks, capital, labor, and market openness have direct positive effects on marine fishery carbon sinks, and the direct effect of innovation is the strongest, and the significance of the indirect effect of the three circles is weaker compared to the direct effect and total effect. As a result, the carbon sink of China’s marine fisheries has a large room for improvement, and in order to promote the high-quality development of China’s marine fisheries, it is still necessary to accelerate the circulation of resources within the marine economic circle, accelerate the research of fishery technology, actively develop the carbon sink fishery, and enhance the specialization of aquatic product processing.
Analyzing Cooking Efficiency of Gradoli Purgatory Beans: Effects of Dehulling, Malting, and Monovalent Carbonates
Authors: Cimini Alessio , Morgante Lorenzo , Moresi Mauro
Published: August 2024

Legumes, rich in protein, fiber, and micronutrients, are increasingly popular in pulse-based and gluten-free foods despite global consumption stagnating at 21 g/day due to taste, low protein digestibility, anti-nutrients, and long cooking times. Bean resistance to cooking causes textural defects like the hardshell and hard-to-cook phenomena. The pectin–cation–phytate hypothesis explains why soaking beans in sodium salts reduces cooking time by enhancing pectin solubility in water. Gradoli Purgatory beans (GPB), from Italy′s Latium region, were malted, reducing phytic acid by 32% and oligosaccharides by 63%. This study evaluated the hardness of cooked GPB seeds in various conditions, including decorticated or malted states, using a modified standard method. Cooking at 98 °C for 7–75 min on an induction hob with a water-to-seed ratio of 4 g/g was tested. Soaking was applied before cooking for conventional seeds only, followed by texture analysis. Conventional GPBs were adequately cooked if their cotyledons disintegrated upon pressing, requiring a force peak of 250 to 220 N and cooking times of 52 to 57 min. Malted, decorticated, and split GPBs cooked similarly to raw decorticated and split ones, with times of 32 and 25 min, respectively. Faster cooking was due to bean coat removal and splitting, not chemical changes. Sodium or potassium carbonate/bicarbonate at 1–2 g/L improved cooking efficiency, with 2 g/L of sodium carbonate reducing cooking time to 13 min. Higher concentrations caused non-uniform cooking. Cooking malted, decorticated, and split GPBs in sodium-carbonated water reduced greenhouse gas emissions from 561 to 368 g CO2e/kg, meeting the demand for eco-friendly and nutritionally enhanced plant protein sources.
Research on Using K-Means Clustering to Explore High-Risk Products with Ethylene Oxide Residues and Their Manufacturers in Taiwan
Authors: Wu Li-Ya , Liu Fang-Ming , Lin Wen-Chou , Qiu Jing-Ting , Lin Hsu-Yang , Lin King-Fu
Published: August 2024

Considering the frequency of ethylene oxide (EtO) residues found in food, the health effects of EtO have become a concern. Between 2022 and 2023, 489 products were inspected using the purposive sampling method in Taiwan, and nine unqualified products were found to have been imported; subsequently, border control measures were enhanced. To ensure the safety of all imported foods, the current study used the K-means clustering method for identifying EtO residues in food. Data on finished products and raw materials with EtO residues from international public opinion bulletins were collected for analysis. After matching them with the Taiwan Food Cloud, 90 high-risk food items with EtO residues and 1388 manufacturers were screened. The Taiwan Food and Drug Administration set up border controls and grouped the manufacturers using K-means clustering in the unsupervised learning algorithm. For this study, 37 manufacturers with priority inspections and 52 high-risk finished products and raw materials with residual EtO were selected for inspection. While EtO was not detected, the study concluded the following: 1. Using international food safety alerts to strengthen border control can effectively ensure domestic food safety; 2. K-means clustering can validate the risk-based purposive sampling results to ensure food safety and reduce costs.
Development of Magnetic Porous Polymer Composite for Magnetic Solid Phase Extraction of Three Fluoroquinolones in Milk
Authors: Yu Zhendong , Xu Tiantian , Lin Shumin , Liang Shuxuan
Published: August 2024

In this study, a magnetic porous polymer composite with both hydrophilic and hydrophobic groups was synthesized for magnetic solid phase extraction (MSPE) of milk substrates. Optimization was conducted on various parameters, including adsorption dose, solution pH, adsorption time, and some elution conditions. Coupled with a high-performance liquid chromatography fluorescence detector, a novel MSPE method for determination of norfloxacin (NFX), ciprofloxacin (CIP), and enrofloxacin (ENR) in milk was developed based on magnetic metal organic framework polystyrene polymer (Fe3O4@MOF@PLS) as adsorbent. The Fe3O4@MOF@PLS exhibited significantly improved adsorption performance compared to MOF and PLS. Under optimized experimental conditions, the method exhibited good linearity for the three fluoroquinolones (FQs) in the range of 0.5–1000 μg/kg, with limit of detections (LODs) ranging from 0.21 to 1.33 μg/kg, and limit of quantitations (LOQs) from 0.71 to 4.42 μg/kg. The relative standard deviation (RSD) for the three FQs were 3.4–8.8%. The recoveries of three FQs in milk samples ranged from 84.2% to 106.2%. This method was successfully applied to the detection of three FQs in 20 types of milk, demonstrating its simplicity, speed, and effectiveness in analyte enrichment and separation. The method presented advantages in adsorbent dosage, adsorption time, LODs, and LOQs, making it valuable for the analysis and detection of FQs in milk.
Naringenin Promotes Gastrointestinal Motility in Mice by Impacting the SCF/c-Kit Pathway and Gut Microbiota
Authors: Wu Lei , Niu Yao , Ren Boyang , Wang Shengyu , Song Yuhong , Wang Xingyu , Zhao Kai , Yue Zhao , Li Yaru , Gao Jianhua
Published: August 2024

Naringenin (NRG) is widely found in citrus fruits and has anti-inflammatory, hypoglycemic, and immunomodulatory effects. Previous studies have shown that NRG promotes gastrointestinal motility in mice constipation models, but there are few systematic evaluations of its effects on normal animals. This study first clarified the promotive effects of NRG on gastric emptying and small intestine propulsion (p < 0.01). NRG can also regulate the release of gastrointestinal hormones, including enhancing gastrin (GAS) and motilin (MTL) (p < 0.01), while reducing vasoactive intestinal peptide (VIP) secretion (p < 0.01). Using NRG to stimulate the isolated stomach, duodenum, and colon showed similar promotive effects to those observed in vivo (p < 0.01). A Western blot analysis indicated that this effect may be mediated by increasing the expression of stem cell factor (SCF) and its receptor (c-Kit) in these three segments, thus regulating their downstream pathways. It is worth noting that NRG can also increase the proportion of beneficial bacteria (Planococcaceae, Bacteroides acidifaciens, Clostridia_UCG-014) in the intestine and reduce the quantity of harmful bacteria (Staphylococcus). These findings provide a new basis for the application of NRG.
Tailoring the Structure and Physico-Chemical Features of Cellulose-Based Hydrogels Using Multi-Epoxy Crosslinking Agents
Authors: Nicu Raluca , Lisa Gabriela , Darie-Nita Raluca Nicoleta , Avadanei Mihaela Iuliana , Bargan Alexandra , Rusu Daniela , Ciolacu Diana Elena
Published: August 2024

Hydrogel features can be designed and optimized using different crosslinking agents to meet specific requirements. In this regard, the present work investigates the physico-chemical features of cellulose-based hydrogels, designed by using different epoxy crosslinkers from the same glycidyl family, namely epichlorohydrin (ECH), 1,4-butanediol diglycidyl ether (BDDE), and trimethylolpropane triglycidyl ether (TMPTGE). The effect of the crosslinker’s structure (from simple to branched) and functionality (mono-, bi- and tri-epoxy groups) on the hydrogels’ features was studied. The performances of the hydrogels were investigated through the gel fraction, as well as by ATR-FTIR, DVS, SEM, DSC, and TG analyses. Also, the swelling and rheological behaviors of the hydrogels were examined. The advantages and limitations of each approach were discussed and a strong correlation between the crosslinker structure and the hydrogel properties was established. The formation of new ether bonds was evidenced by ATR-FTIR spectroscopy. It was emphasized that the pore size is directly influenced by the crosslinker type, namely, it decreases with the increasing number of epoxy groups from the crosslinker molecule, i.e., from 46 ± 11.1 µm (hydrogel CE, with ECH) to 12.3 ± 2.5 µm (hydrogel CB, with BDDE) and 6.7 ± 1.5 µm (hydrogel CT, with TMPTGE). The rheological behavior is consistent with the swelling data and hydrogel morphology, such as CE with the highest Qmax and the largest pore size being relatively more elastic than CB and CT. Instead, the denser matrices obtained by using crosslinkers with more complex structures have better thermal stability. The experimental results highlight the possibility of using a specific crosslinking agent, with a defined structure and functionality, in order to establish the main characteristics of hydrogels and, implicitly, to design them for a certain field of application.
Enhancement of Mechanical Properties of Benign Polyvinyl Alcohol/Agar Hydrogel by Crosslinking Tannic Acid and Applying Multiple Freeze/Thaw Cycles
Authors: Mahamoud Moustapha Mohamed , Ketema Tadesse Mekonnin , Kuwahara Yutaka , Takafuji Makoto
Published: August 2024

Hydrogels composed of natural and synthetic polymers have considerable potential for use in diverse areas such as biomedical applications and water purification. This is primarily because of their biocompatibility, biodegradability, and low toxicity. The widespread usage of composite hydrogels is hindered by a lack of simultaneous properties, such as high strength and low swelling rate. Herein, we report the preparation of novel hydrogels composed of polyvinyl alcohol (PVA)–intercalated agar polymer networks physically crosslinked with tannic acid. The hydrogel was subjected to multiple freeze/thaw (F/T) cycles (1, 3, and 5), and it was found to exhibit the highest strength after 5 F/T cycles. After 1 F/T cycle, the tensile strength of the composite hydrogel reached 1.56 MPa with a 1.0 wt% crosslinker, whereas after 5 F/T cycles, it increased to 3.77 MPa with a reduced amount (0.75 wt%) of the crosslinker. In addition, the swelling ability decreased upon increasing the crosslinker content and number of F/T cycles. Furthermore, the hydrogel demonstrated excellent water retention and a strong ability to adhere to different substrates. We have successfully implemented an innovative approach to improve the mechanical properties of PVA-based hydrogels by combining the use of tannic acid as a cross-linking agent and multiple F/T cycles. The developed hydrogels are expected to facilitate new developments in hydrogel technology, thus impacting diverse fields such as biomedical (wound dressing and artificial cartilage).
Screening of Optimal Konjac Glucomannan–Protein Composite Gel Formulations to Mimic the Texture and Appearance of Tripe
Authors: Zou Qiang , Liu Yudie , Luo Linghui , Chen Yuyou , Zheng Yuhan , Ran Guilian , Liu Dayu
Published: August 2024

This study aimed to develop a product that closely replicates the texture and appearance of tripe. The effect of three different proteins (soy protein isolate (SPI), pea protein isolate (PPI), and whey protein isolate (WPI)) at different protein levels and processing conditions (heating (90 °C, 1 h) followed by cooling (4 °C, 12 h) and heating (90 °C, 1h) followed by freezing (−18 °C, 12 h)) of konjac glucomannan (KGM) was analyzed. The optimal formulations for simulating tripe were screened by examining their similarity to real tripe in terms of texture, color, and sensory experience. The screened formulations were also subjected to a preliminary mechanistic investigation. The results show that all three proteins improved the gel’s textural properties to varying degrees. At the same concentration, the hardness and chewiness of the KGM/WPI composite gel were significantly higher than those of the other two KGM/protein composite gels, among which the composite gel obtained by adding 8% WPI and 5% KGM heating-frozen (FWK4) had the greatest hardness and chewiness of 4338.07 g and 2313.76, respectively, and the springiness differences in all of the composite gels were small. In addition, the addition of protein increased the whiteness of the hybrid gels, with WPI having the most significant effect on the whiteness of the composite gels (whiteness increased from 30.25 to 62.80 as the concentration of WPI increased from 0 to 10%). Freezing increased composite gel hardness and chewiness, but reduced gel springiness and whiteness. Cluster analysis showed that the composite gel obtained by heating–cooling 8% WPI and 5% KGM (WK4) was very similar to the real tripe in terms of chewiness and whiteness, and WK4 had the highest sensory scores for color, tissue morphology, tactile sensation, taste, and odor. The acceptability score in terms of tissue morphology reached 4.3. Meanwhile, the characterization results of WK4 indicate the presence of large junction areas in the gel network. Fourier transform infrared spectroscopy (FTIR) analysis, X-ray diffraction, and intermolecular force contributions indicated that the incorporation of WPI promoted integral interactions, and that hydrophobic interactions and disulfide bonding played a key role in the WK4 composite gel system. Moreover, scanning electron microscopy (SEM) also showed that the combination of WPI and konjac glucan resulted in a more compact gel structure. This study is informative for the development of the field of bionic tripe processing.
Moisture Sorption Isotherms of Polydextrose and Its Gelling Efficiency in Inhibiting the Retrogradation of Rice Starch
Authors: Liu Chang , Li Xiaoyu , Song Hongdong , Li Xingjun
Published: August 2024

As an anti-staling agent in bread, the desorption isotherm of polydextrose has not been studied due to a very long equilibrium time. The adsorption and desorption isotherms of five Chinese polydextrose products were measured in the range of 0.1–0.9 aw and 20–35 °C by a dynamic moisture sorption analyzer. The results show that the shape of adsorption and desorption isotherms was similar to that of amorphous lactose. In the range of 0.1–0.8 aw, the hysteresis between desorption and adsorption of polydextrose was significant. The sorption isotherms of polydextrose can be fitted by seven commonly used models, and our developed seven-parameter polynomial, the adsorption equations of generalized D’Arcy and Watt (GDW) and Ferro-Fontan, and desorption equations of polynomial and Peleg, performed well in the range of 0.1–0.9 aw. The hysteresis curves of polydextrose at four temperatures quickly decreased with aw increase at aw ˂ 0.5, andthereafter slowly decreased when aw ≥ 0.5. The polynomial fitting hysteresis curves of polydextrose were divided into three regions: ˂0.2, 0.2–0.7, and 0.71–0.9 aw. The addition of 0–10% polydextrose to rice starch decreased the surface adsorption and bulk absorption during the adsorption and desorption of rice starch, while it increased the water adsorption value at aw ≥ 0.7 due to polydextrose dissolution. DSC analysis showed that polydextrose as a gelling agent inhibited the retrogradation of rice starch, which could be used to maintain the quality of cooked rice.
The Impact of Gel Parameters on the Dispersal and Fragmentation of Hyaluronic Acid Gel Fillers within an Artificial Model of Arterial Embolism
Authors: Soares Danny J. , McCarthy Alec D.
Published: August 2024

Accidental arterial embolization of hyaluronic acid (HA) fillers can lead to severe complications, including skin ischemia, blindness, and stroke. Currently, the intra-arterial dispersal and fragmentation behavior of HA gels is unknown but critical to our understanding of the pathomechanism of these injuries. This work introduces the Pulsatile Unit for the Laboratory Simulation of Arterio-embolic Restrictions (PULSAR) and evaluates the intravascular behavior of different HA gels. The fragmentation and dispersal behaviors of four HA gels with distinct rheological properties were evaluated via high-resolution videography and ImageJ particle size and morphology analysis. The gels’ elastic modulus (G′), loss modulus (G″), tan(δ), and HA concentration were subsequently correlated with their intra-arterial behaviors. This study effectively confirms the extensive fragmentation of HA gels upon arterial inoculation, with particle sizes ranging from <50 µm to >1 mm. Gel particle size and morphology correlated most significantly with tan(δ). Conversely, arterial flow rates did not significantly influence gel fragmentation behavior, though the probability of proximal, macrovascular obstruction was affected. Overall, this study validates the PULSAR model for simulation of arterial dynamics and the testing of intravascular filler kinematics. The findings demonstrate the ability of gels to microfragment and disseminate distally, as well as induce partial proximal occlusion depending on gel rheology and arterial flow parameters.
“Go to the Attics, the Closets, and the Basements”: Black Women’s Intergenerational Practices of Memory Keeping in Oxford, Ohio
Authors: Sutton Jazma
Published: August 2024

In 1993, eighty-nine-year-old Jennie Eunice Elder Suel, from Oxford, Ohio, donated a collection of personal and family documents to Miami University’s Walter Havighurst Special Collections. This article examines the Jennie Elder Suel Collection and the actions made by multiple generations of Black women, who chose to preserve their history. The first section traces the development of Suel’s collection and the way in which it is preserved in local archives today. The second section situates the Suel family in a wider context and discusses the archival challenges of recovering the lives and experiences of antebellum Black women in the Midwest, and the following section explores how I have attempted to navigate these challenges through a research method I have innovated called Descendant Archival Practices (DAP). The remainder of the article offers a careful analysis of Black women’s home-based archives and their implications for understanding nineteenth-century Black women’s motivations for archiving themselves. Part of this assessment includes analyzing which records these women deemed valuable to preserve, revealing the inner lives of Black women and the things they cherished. Through these deliberate and heartfelt choices, Black women ensured their legacy through the preservation of their ancestral history.
Canine Histiocytic and Hemophagocytic Histiocytic Sarcomas Display KRAS and Extensive PTPN11/SHP2 Mutations and Respond In Vitro to MEK Inhibition by Cobimetinib
Authors: Yang Ya-Ting , Engleberg Alexander I. , Kapoor Ishana , Kitagawa Keita , Hilburger Sara A. , Thaiwong-Nebelung Tuddow , Yuzbasiyan-Gurkan Vilma
Published: August 2024

Histiocytic sarcoma (HS) is a rare and highly aggressive cancer in humans and dogs. In dogs, it has a high prevalence in certain breeds, such as Bernese mountain dogs (BMDs) and flat-coated retrievers. Hemophagocytic histiocytic sarcoma (HHS) is a unique form of HS that presents with erythrophagocytosis. Due to its rareness, the study of HHS is very limited, and mutations in canine HHS patients have not been studied to date. In previous work, our research group identified two major PTPN11/SHP2 driver mutations, E76K and G503V, in HS in dogs. Here, we report additional mutations located in exon 3 of PTPN11/SHP2 in both HS and HHS cases, further supporting that this area is a mutational hotspot in dogs and that mutations in tumors and liquid biopsies should be evaluated utilizing comprehensive methods such as Sanger and NextGen sequencing. The overall prevalence of PTPN11/SHP2 mutations was 55.8% in HS and 46.2% in HHS. In addition, we identified mutations in KRAS, in about 3% of HS and 4% of HHS cases. These findings point to the shared molecular pathology of activation of the MAPK pathway in HS and HHS cases. We evaluated the efficacy of the highly specific MEK inhibitor, cobimetinib, in canine HS and HHS cell lines. We found that the IC50 values ranged from 74 to 372 nM, which are within the achievable and tolerable ranges for cobimetinib. This finding positions cobimetinib as a promising potential candidate for future canine clinical trials and enhances our understanding of the molecular defects in these challenging cancers.
The Phenotypic Spectrum of 16p11.2 Recurrent Chromosomal Rearrangements
Authors: Mitrakos Anastasios K. , Kosma Konstantina , Makrythanasis Periklis , Tzetis Maria
Published: August 2024

The human 16p11.2 chromosomal region is rich in segmental duplications which mediate the formation of recurrent CNVs. CNVs affecting the 16p11.2 region are associated with an increased risk for developing neuropsychiatric disorders, including autism spectrum disorder (ASD), schizophrenia, and intellectual disability (ID), as well as abnormal body weight and head circumference and dysmorphic features, with marked phenotypic variability and reduced penetrance. CNVs affecting the 16p11.2 region mainly affect a distal interval of ~220 Kb, between Breakpoints 2 and 3 (BP2–BP3), and a proximal interval of ~593 Kb (BP4–BP5). Here, we report on 15 patients with recurrent 16p11.2 rearrangements that were identified among a cohort of 1600 patients (0.9%) with neurodevelopmental disorders. A total of 13 deletions and two duplications were identified, of which eight deletions included the proximal 16p11.2 region (BP4–BP5) and five included the distal 16p11.2 region (BP2–BP3). Of the two duplications that were identified, one affected the proximal and one the distal 16p11.2 region; however, both patients had additional CNVs contributing to phenotypic severity. The features observed and their severity varied greatly, even between patients within the same family. This article aims to further delineate the clinical spectrum of patients with 16p11.2 recurrent rearrangements in order to aid the counselling of patients and their families.
Bioinformatic Evaluation of KLF13 Genetic Variant: Implications for Neurodevelopmental and Psychiatric Symptoms
Authors: Vinci Mirella , Greco Donatella , Treccarichi Simone , Chiavetta Valeria , Figura Maria Grazia , Musumeci Antonino , Greco Vittoria , Federico Concetta , Calì Francesco , Saccone Salvatore
Published: August 2024

The Krüppel-like factor (KLF) family represents a group of transcription factors (TFs) performing different biological processes that are crucial for proper neuronal function, including neuronal development, synaptic plasticity, and neuronal survival. As reported, genetic variants within the KLF family have been associated with a wide spectrum of neurodevelopmental and psychiatric symptoms. In a patient exhibiting attention deficit hyperactivity disorder (ADHD) combined with both neurodevelopmental and psychiatric symptoms, whole-exome sequencing (WES) analysis revealed a de novo heterozygous variant within the Krüppel-like factor 13 (KLF13) gene, which belongs to the KLF family and regulates axonal growth, development, and regeneration in mice. Moreover, in silico analyses pertaining to the likely pathogenic significance of the variant and the impact of the mutation on the KLF13 protein structure suggested a potential deleterious effect. In fact, the variant was localized in correspondence to the starting residue of the N-terminal domain of KLF13, essential for protein–protein interactions, DNA binding, and transcriptional activation or repression. This study aims to highlight the potential involvement of the KLF13 gene in neurodevelopmental and psychiatric disorders. Nevertheless, we cannot rule out that excluded variants, those undetectable by WES, or the polygenic risk may have contributed to the patient’s phenotype given ADHD’s high polygenic risk. However, further functional studies are required to validate its potential contribution to these disorders.
Effects of ACLY Inhibition on Body Weight Distribution: A Drug Target Mendelian Randomization Study
Authors: Gill Dipender , Dib Marie-Joe , Gill Rubinder , Bornstein Stefan R. , Burgess Stephen , Birkenfeld Andreas L.
Published: August 2024

Background: Adenosine triphosphate-citrate lyase (ACLY) inhibition has proven clinically efficacious for low-density lipoprotein cholesterol (LDL-c) lowering and cardiovascular disease (CVD) risk reduction. Clinical and genetic evidence suggests that some LDL-c lowering strategies, such as 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) inhibition with statin therapy increase body weight and the risk of developing type 2 diabetes mellitus (T2DM). However, whether ACLY inhibition affects metabolic risk factors is currently unknown. We aimed to investigate the effects of ACLY inhibition on glycaemic and anthropometric traits using Mendelian randomization (MR). Methods: As genetic instruments for ACLY inhibition, we selected weakly correlated single-nucleotide polymorphisms at the ACLY gene associated with lower ACLY gene expression in the eQTLGen study (N = 31,684) and lower LDL-c levels in the Global Lipid Genetic Consortium study (N = 1.65 million). Two-sample Mendelian randomization was employed to investigate the effects of ACLY inhibition on T2DM risk, and glycaemic and anthropometric traits using summary data from large consortia, with sample sizes ranging from 151,013 to 806,834 individuals. Findings for genetically predicted ACLY inhibition were compared to those obtained for genetically predicted HMGCR inhibition using the same instrument selection strategy and outcome data. Results: Primary MR analyses showed that genetically predicted ACLY inhibition was associated with lower waist-to-hip ratio (β per 1 standard deviation lower LDL-c: −1.17; 95% confidence interval (CI): −1.61 to −0.73; p < 0.001) but not with risk of T2DM (odds ratio (OR) per standard deviation lower LDL-c: 0.74, 95% CI = 0.25 to 2.19, p = 0.59). In contrast, genetically predicted HMGCR inhibition was associated with higher waist-to-hip ratio (β = 0.15; 95%CI = 0.04 to 0.26; p = 0.008) and T2DM risk (OR = 1.73, 95% CI = 1.27 to 2.36, p < 0.001). The MR analyses considering secondary outcomes showed that genetically predicted ACLY inhibition was associated with a lower waist-to-hip ratio adjusted for body mass index (BMI) (β = −1.41; 95%CI = −1.81 to −1.02; p < 0.001). In contrast, genetically predicted HMGCR inhibition was associated with higher HbA1c (β = 0.19; 95%CI = 0.23 to 0.49; p < 0.001) and BMI (β = 0.36; 95%CI = 0.23 to 0.49; p < 0.001). Conclusions: Human genetic evidence supports the metabolically favourable effects of ACLY inhibition on body weight distribution, in contrast to HMGCR inhibition. These findings should be used to guide and prioritize ongoing clinical development efforts.