bluuebunny/crossref_metadata_2025 huggingface dataset:
Fractal Characterization of Nanopore Structure in Shale, Tight Sandstone and Mudstone from the Ordos Basin of China Using Nitrogen Adsorption
Authors: Li Xiaohong , Gao Zhiyong , Fang Siyi , Ren Chao , Yang Kun , Wang Fuyong
Published: February 2019

The characteristics of the nanopore structure in shale, tight sandstone and mudstone from the Ordos Basin of China were investigated by X-ray diffraction (XRD) analysis, porosity and permeability tests and low-pressure nitrogen adsorption experiments. Fractal dimensions D1 and D2 were determined from the low relative pressure range (0 < P/P0 < 0.4) and the high relative pressure range (0.4 < P/P0 < 1) of nitrogen adsorption data, respectively, using the Frenkel–Halsey–Hill (FHH) model. Relationships between pore structure parameters, mineral compositions and fractal dimensions were investigated. According to the International Union of Pure and Applied Chemistry (IUPAC) isotherm classification standard, the morphologies of the nitrogen adsorption curves of these 14 samples belong to the H2 and H3 types. Relationships among average pore diameter, Brunner-Emmet-Teller (BET) specific surface area, pore volume, porosity and permeability have been discussed. The heterogeneities of shale nanopore structures were verified, and nanopore size mainly concentrates under 30 nm. The average fractal dimension D1 of all the samples is 2.1187, varying from 1.1755 to 2.6122, and the average fractal dimension D2 is 2.4645, with the range from 2.2144 to 2.7362. Compared with D1, D2 has stronger relationships with pore structure parameters, and can be used for analyzing pore structure characteristics.
Influence on CO and PM Emissions of an Innovative Burner Pot for Pellet Stoves: An Experimental Study
Authors: Polonini Luigi F. , Petrocelli Domenico , Parmigiani Simone P. , Lezzi Adriano M.
Published: February 2019

In this study, an extensive set of experiments has been performed to investigate how the emissions of carbon monoxide (CO) and particulate matter (PM) from wood pellet combustion are correlated with the different layout of air inlets in the two burner pots of pellet stoves. In particular, emissions generated using a “traditional” burner pot are compared with those produced with an innovative one. Tests were carried out on four pellet stoves (8–11 kW burning power). Tests show that CO emissions can be reduced close to zero using the innovative burner pot. PM emissions are also reduced, but not as much as those of CO. This phenomenon is explained assuming that CO reduction implies a reduction of the organic part of PM, but not of the inorganic matter. Experiments also show an apparent dependence of PM release on the time from ignition for both burner pot models: PM emissions increase and the color of PM deposited on filters varies over a wide range. It is argued that this effect is due to ash accumulation on the bottom of the burner pot and to its interaction with the primary air stream that flows over it.
A Study on the Effects of Energy and Environmental Efficiency at China’s Provincial Level
Authors: Ma Liang-han , Hsieh Jin-chi , Chiu Yung-ho
Published: February 2019

China’s economic development status continues to grow, but its environmental degradation issue is also becoming a global concern. This study uses dynamic network data envelopment analysis (DN-DEA) to evaluate the energy using efficiency and environmental efficiency in China over the period 2014–2017. The result shows that the former is greater than the latter for all years. This study utilizes a policy-oriented matrix to find the relationship between energy efficiency and Malmquist productivity index (MPI), showing that Chongqing and 13 other provinces have relatively poor energy efficiency, and they therefore must formulate a more effective energy policy to improve undesirable gas emissions. Shanghai and 6 other provinces exhibit relatively good energy efficiency, but are not progressing in MPI, and hence they must develop a stable energy strategy to avoid different efficiencies of catch-up and frontier-shift across time periods. High energy-consuming industries must also choose a low-carbon energy strategy so that they can promote economic development, while taking into account environmental protection in China’s provincial level.
Integrated Planning for Regional Electric Power System Management with Risk Measure and Carbon Emission Constraints: A Case Study of the Xinjiang Uygur Autonomous Region, China
Authors: Xie Yulei , Fu Zhenghui , Xia Dehong , Lu Wentao , Huang Guohe , Wang Han
Published: February 2019

With the carbon reduction targets being set in the Paris Agreement on Climate Change, China is facing great pressure to meet its emission reduction commitment. The electric power industry as the major source of carbon emissions needs to be a focus. However, the uncertainty of power systems, the risk of reducing emissions and the fuzziness of carbon capture technology popularization rate and carbon reduction targets makes previous planning methods unsatisfactory for current planning. This paper establishes an interval fuzzy programming with a risk measure model which takes carbon capture technology and carbon reduction targets into account, to ensure that the complex electric management system achieves the best developmental state. It was concluded that in order to reduce carbon emissions, wind power and hydropower would be the best choices, and coal-fired power would be the suboptimal choice, and solar power would play a complementary role. Besides, decision makers should put much more effort into promoting and improving carbon capture technology instead of simply setting emission reduction targets. The non-synchronism of the downward trend in carbon emissions per unit of electricity generation and electric power industry total carbon emissions need to be taken seriously.
Power Stabilization based on Switching Control of Segmented Transmitting Coils for Multi Loads in Static-Dynamic Hybrid Wireless Charging System at Traffic Lights
Authors: Liu Han , Tan Linlin , Huang Xueliang , Zhang Ming , Zhang Zhenxing , Li Jiacheng
Published: February 2019

In order to promote the driving range of the electric vehicles (EVs), decrease the demand for large capacity battery and create more charging opportunities for the EVs, the static-dynamic hybrid wireless charging system is presented to utilize waiting time of traffic lights at intersections to replenish electricity in this paper. Firstly, the topology and principle of the proposed static-dynamic hybrid wireless charging system with short-segmented coils at traffic lights are introduced. Secondly, the general circuit model of the static-dynamic hybrid wireless charging system with multi-coupling between the primary and secondary sides is developed. The design method of the compensation circuit in primary side based on the number and the positions of the energized primary coils is investigated. After analyzing the characteristics of the system varying with the position of the single load, the control conceptions of the energizing mode alternating in primary side and the combination of the primary compensation capacitors are put forward. Then the power stabilization control strategy of the static-dynamic hybrid wireless charging system with multi short-segmented coils and multi loads is proposed. Finally, the theoretical analyses are verified with the experiments.
Do High-Voltage Power Transmission Lines Affect Forest Landscape and Vegetation Growth: Evidence from a Case for Southeastern of China
Authors: Li Xiang , Lin Yuying
Published: February 2019

The rapid growth of the network of high-voltage power transmission lines (HVPTLs) is inevitably covering more forest domains. However, no direct quantitative measurements have been reported of the effects of HVPTLs on vegetation growth. Thus, the impacts of HVPTLs on vegetation growth are uncertain. Taking one of the areas with the highest forest coverage in China as an example, the upper reaches of the Minjiang River in Fujian Province, we quantitatively analyzed the effect of HVPTLs on forest landscape fragmentation and vegetation growth using Landsat imageries and forest inventory datasets. The results revealed that 0.9% of the forests became edge habitats assuming a 150 m depth-of-edge-influence by HVPTLs, and the forest plantations were the most exposed to HVPTLs among all the forest landscape types. Habitat fragmentation was the main consequence of HVPTL installation, which can be reduced by an increase in the patch density and a decrease in the mean patch area (MA), largest patch index (LPI), and effective mesh size (MESH). In all the landscape types, the forest plantation and the non-forest land were most affected by HVPTLs, with the LPI values decreasing by 44.1 and 20.8%, respectively. The values of MESH decreased by 44.2 and 32.2%, respectively. We found an obvious increasing trend in the values of the normalized difference vegetation index (NDVI) in 2016 and NDVI growth during the period of 2007 to 2016 with an increase in the distance from HVPTL. The turning points of stability were 60 to 90 meters for HVPTL corridors and 90 to 150 meters for HVPTL pylons, which indicates that the pylons have a much greater impact on NDVI and its growth than the lines. Our research provides valuable suggestions for vegetation protection, restoration, and wildfire management after the construction of HVPTLs.
Use of a Selected Leuconostoc Citreum Strain as a Starter for Making a “Yeast-Free” Bread
Authors: De Bellis Palmira , Rizzello Carlo , Sisto Angelo , Valerio Francesca , Lonigro Stella , Conte Amalia , Lorusso Valeria , Lavermicocca Paola
Published: February 2019

The aim of this study was the characterization and selection of bacterial strains suitable for the production of a “yeast-free” bread. The strains Leuconostoc citreum C2.27 and Weissella confusa C5.7 were selected for their leavening and acidification capabilities and individually used as starters in bread-making tests. Liquid type-II sourdoughs, singly inoculated with the two selected strains, were characterized and employed for bread-making, through the set-up of a biotechnological protocol without the use of baker’s yeast as a leavening agent. Aiming to verify the ability of the selected strains to dominate the fermentation process, bacteria and yeasts were isolated from liquid sourdoughs and doughs, genetically characterized and identified. Both the selected strains were suitable for the production of bread, even if L. citreum C2.27 showed the highest leavening capacity and was able to dominate the dough microbiota. The effects of different salt concentrations on the selected strain performances were also investigated. The applicability of the developed protocol, adapted for the production of the typical Apulian bread, “puccia”, and the suitability of the strain L. citreum C2.27 were confirmed at pilot scale in an industrial bakery. The puccia bread, which was produced with the liquid sourdough fermented with L. citreum C2.27, without baker’s yeast and salt, was similar in appearance to the conventional product containing baker’s yeast and was judged positively by a sensory analysis.
The Occurrence of Biogenic Amines and Determination of Biogenic Amine-Producing Lactic Acid Bacteria in Kkakdugi and Chonggak Kimchi
Authors: Jin Young Hun , Lee Jae Hoan , Park Young Kyung , Lee Jun-Hee , Mah Jae-Hyung
Published: February 2019

In this study, biogenic amine content in two types of fermented radish kimchi (Kkakdugi and Chonggak kimchi) was determined by high performance liquid chromatography (HPLC). While most samples had low levels of biogenic amines, some samples contained histamine content over the toxicity limit. Additionally, significant amounts of total biogenic amines were detected in certain samples due to high levels of putrefactive amines. As one of the significant factors influencing biogenic amine content in both radish kimchi, Myeolchi-aekjoet appeared to be important source of histamine. Besides, tyramine-producing strains of lactic acid bacteria existed in both radish kimchi. Through 16s rRNA sequencing analysis, the dominant species of tyramine-producing strains was identified as Lactobacillus brevis, which suggests that the species is responsible for tyramine formation in both radish kimchi. During fermentation, a higher tyramine accumulation was observed in both radish kimchi when L. brevis strains were used as inocula. The addition of Myeolchi-aekjeot affected the initial concentrations of histamine and cadaverine in both radish kimchi. Therefore, this study suggests that reducing the ratio of Myeolchi-aekjeot to other ingredients (and/or using Myeolchi-aekjeot with low biogenic amine content) and using starter cultures with ability to degrade and/or inability to produce biogenic amines would be effective in reducing biogenic amine content in Kkakdugi and Chonggak kimchi.
Overexpression of StDREB2 Transcription Factor Enhances Drought Stress Tolerance in Cotton (Gossypium barbadense L.)
Authors: El-Esawi Mohamed A. , Alayafi Aisha A.
Published: February 2019

Drought stress significantly restricts plant growth and crop productivity. Cotton is the most important textile fiber and oilseed crop worldwide, and its cultivation is affected by drought stress, particularly in dry regions. Improving cotton tolerance to drought stress using the advanced genetic engineering technologies is a promising strategy to maintain crop production and fiber quality and meet the increasing worldwide fiber and oil demand. Dehydration-responsive element binding (DREB) transcription factors play a main role in regulating stresses-tolerance pathways in plant. This study investigated whether potato DREB2 (StDREB2) overexpression can improve drought tolerance in cotton. StDREB2 transcription factor was isolated and overexpressed in cotton. Plant biomass, boll number, relative water content, soluble sugars content, soluble protein content, chlorophyll content, proline content, gas-exchange parameters, and antioxidants enzymes (POD, CAT, SOD, GST) activity of the StDREB2-overexpressing cotton plants were higher than those of wild type plants. By contrast, the contents of malondialdehyde, hydrogen peroxide and superoxide anion of StDREB2-overexpressing transgenic plants were significantly lower than that of the wild type plants. Moreover, the transgenic cotton lines revealed higher expression levels of antioxidant genes (SOD, CAT, POD, GST) and stress-tolerant genes (GhERF2, GhNAC3, GhRD22, GhDREB1A, GhDREB1B, GhDREB1C) compared to wild-type plants. Taken together, these findings showed that StDREB2 overexpression augments drought stress tolerance in cotton by inducing plant biomass, gas-exchange characteristics, reactive oxygen species (ROS) scavenging, antioxidant enzymes activities, osmolytes accumulation, and expression of stress-related genes. As a result, StDREB2 could be an important candidate gene for drought-tolerant cotton breeding.
Molecular Evolution of the Vacuolar Iron Transporter (VIT) Family Genes in 14 Plant Species
Authors: Cao Jun
Published: February 2019

The vacuolar iron transporter (VIT) proteins are involved in the storage and transport of iron. However, the evolution of this gene family in plants is unknown. In this study, I first identified 114 VIT genes in 14 plant species and classified these genes into seven groups by phylogenetic analysis. Conserved gene organization and motif distribution implied conserved function in each group. I also found that tandem duplication, segmental duplication and transposition contributed to the expansion of this gene family. Additionally, several positive selection sites were identified. Divergent expression patterns of soybean VIT genes were further investigated in different development stages and under iron stress. Functional network analysis exhibited 211 physical or functional interactions. The results will provide the basis for further functional studies of the VIT genes in plants.
Rhyolite Domes and Subsequent Offlap of Pliocene Carbonates on Volcanic Islets at San Basilio (Baja California Sur, Mexico)
Authors: Johnson Markes E. , Backus David H. , Carreño Ana Luisa , Ledesma-Vázquez Jorge
Published: February 2019

San Basilio basin in Baja California Sur (Mexico) exhibits distinct styles of volcanism that interrupted phases of normal sedimentation correlated with the Zanclean Stage (Lower Pliocene). Sea cliffs around a 4-km2 bay opening onto the Gulf of California are dominated by rhyolite, mudstone, sandstone, and limestone. Volcanism associated with re-sedimented hyaloclastite is regionally uncommon and the goal was to investigate interactions between volcanic events and intervals of stability represented by fossil-rich strata. Methods of study involved a combination of microfossil and macrofossil analyses. Relating the basin’s faults to Pliocene development in the greater Gulf of California was a secondary goal. Microfossils Bolivina bicostata and B. interjuncta recovered from mudstone indicate an initial water column of 150 m. An abrupt hydromagmatic explosion ruptured the mudstone cover, followed by banded rhyolite flows inter-bedded with sandstone. Outlying limestone beds with the index fossil Clypeaster bowersi are separated from rhyolite by conglomerate eroded under intertidal conditions. A renewed phase of activity saw eruption of smaller volcanoes in the basin center semi-contemporaneous with pecten limestone deposited on unstable slopes. Normal faults conform to a pattern of extensional rifting in the proto-gulf, followed by cross-cutting faults indicating the onset of transtensional tectonics beginning about 3.5 Ma.
Architectural Heritage: A Discussion on Conservation and Safety
Authors: Borri Antonio , Corradi Marco
Published: February 2019

This paper presents a study carried out in the Italian region of Umbria, after the 2016 Central Italy earthquake. The aim is to address the problem of the conservation and protection of architectural heritage in Italy. The application of retrofitting strategies, while improving the seismic performance of historic—and frequently listed by conservation bodies—buildings, will not significantly alter their appearance, will be reversible, and fall within the principle of minimum intervention (minimal (least) intervention (or conservative repair)). These issues were evaluated in the paper by considering the different points of view of structural engineers and conservators. Heavy structural-oriented interventions as well as the underestimation of the importance of the structural safety for masonry monuments has produced, in the past, irreversible damage to important buildings and monuments, and loss of architectural heritage in Italy. The requirement of structural safety, especially for monuments located in seismic-prone areas, is compared with the need for conservation. The Italian building guidelines for interventions of cultural heritage are also discussed, highlighting some limitations and important advancements.
Experiences with Participation in a Supervised Group-Based Outdoor Cycling Programme for People with Mental Illness: A Focus Group Study
Authors: Schnor Helle , Linderoth Stina , Midtgaard Julie
Published: February 2019

Epidemiological evidence suggests that physical exercise, notably popular sports, is associated with reduced, mental health burden. This study explored participation in a supervised, group-based, outdoor cycling programme (10 × 10 km rides over a five-month period) for people with mental illness. We conducted two rounds of three audio-taped focus groups with people with mental illness (n = 25, mean age = 40 years) that focused on previous physical activity and motivation for enrolment (baseline), and on programme evaluation, including subjective wellbeing (after 10 weeks). Transcribed verbatim, the group discussions were analysed using systematic text condensation, which identified 12 categories and four themes: 1) Reinvigoration, (2) motivation through equal status, (3) group commitment without focus on illness, and (4) the value of cycling. Of particular interest was the potential for outdoor cycling to support unique non-stigmatising therapeutic relationships in a non-patient environment, outdoor sensory experiences, e.g., fresh air, wind, and rain, and feelings of personal mastery, equal status, solidarity, community, and healing. This study indicated that outdoor cycling performed in groups supervised by healthcare staff may support exercise self-efficacy and empower people with mental illness, potentially promoting long-term physical activity and participation. Future interventional studies examining the effectiveness of outdoor cycling complementary to conventional community mental healthcare services are warranted.
Truth Telling about Tobacco and Nicotine
Authors: Annechino Rachelle , Antin Tamar M.J.
Published: February 2019

Research suggests that many people in the US are misinformed about the relative harms of various tobacco and nicotine products. Concerns about public misinformation have often been framed as relevant only to the degree that public health institutions agree to prioritize conventional approaches to tobacco harm reduction. We argue that while the information priorities of public health professionals are important, ethical and credible information sharing also requires consideration of broader issues related to public trust. To promote trust, public health institutions must develop truth telling relationships with the communities they serve and be genuinely responsive to what people themselves want to know about tobacco and nicotine products.
Physical Activity of Workers in a Hospital
Authors: Jun So Yeon , Kim Jaewon , Choi Hyehoon , Kim Joon Sung , Lim Seong Hoon , Sul Bomi , Hong Bo Young
Published: February 2019

Purpose: This study aims to evaluate the physical activity of healthcare personnel and the affecting factors of physical activity (PA) in a hospital using an accelerometer device (Actigraph wGT3X-BT). Method: A total of 63 subjects (22 physicians, 19 nurses, and 23 supporting staff) participated and wore an accelerometer for seven days. Among the outputs, the mean counts for a minute, time spent for light, moderate, and vigorous intensity PA, and step count were extracted. As a secondary study, 16 subjects continued for one more week after feedback on their PA of the previous week and counseling to encourage PA. Result: Most of (62/63) the participants fulfilled the recommended amount of PA, which is more than 300 min of moderate to vigorous physical activity (MVPA). Physicians showed significantly less PA than nurses or supporting staffs: Mean counts per minute (210.4 vs. 476.0 and 441.8 respectively), time in MVPA per week (904.7 min vs. 1471.3 min and 1451.0 min), and step counts per week (69,029 vs. 87,119 and 84,700) (p < 0.001). Nurses and supporting staff were not statistically different. There was no significant difference in the PA of workers in the hospital regarding gender and marital status. However, the average calorie expenditure of the child raising group was significantly higher. There was no statistically significant difference in PA before and after counseling. No participants reported a vigorous degree of exercise intensity over the study period. Conclusion: Most of the healthcare personnel met the recommended PA, however, only 57% (36/63) recalled having engaged in MVPA during the study period. The group of physicians showed less PA compared to nurses or supporting staff. Single check-up and counseling were not found to increase PA.
Chemical Composition and Source Apportionment of PM2.5 in Urban Areas of Xiangtan, Central South China
Authors: Ma Xiaoyao , Xiao Zhenghui , He Lizhi , Shi Zongbo , Cao Yunjiang , Tian Zhe , Vu Tuan , Liu Jisong
Published: February 2019

Xiangtan, South China, is characterized by year-round high relative humidity and very low wind speeds. To assess levels of PM2.5, daily samples were collected from 2016 to 2017 at two urban sites. The mass concentrations of PM2.5 were in the range of 30–217 µg/m3, with the highest concentrations in winter and the lowest in spring. Major water-soluble ions (WSIIs) and total carbon (TC) accounted for 58–59% and 21–24% of the PM2.5 mass, respectively. Secondary inorganic ions (SO42−, NO3−, and NH4+) dominated the WSIIs and accounted for 73% and 74% at the two sites. The concentrations of K, Fe, Al, Sb, Ca, Zn, Mg, Pb, Ba, As, and Mn in the PM2.5 at the two sites were higher than 40 ng/m3, and decreased in the order of winter > autumn > spring. Enrichment factor analysis indicates that Co, Cu, Zn, As, Se, Cd, Sb, Tl, and Pb mainly originates from anthropogenic sources. Source apportionment analysis showed that secondary inorganic aerosols, vehicle exhaust, coal combustion and secondary aerosols, fugitive dust, industrial emissions, steel industry are the major sources of PM2.5, contributing 25–27%, 21–22%, 19–21%, 16–18%, 6–9%, and 8–9% to PM2.5 mass.
Chronic Obstructive Pulmonary Disease as a Main Factor of Premature Aging
Authors: Karametos Ilias , Tsiboli Paraskevi , Togousidis Ilias , Hatzoglou Chrisi , Giamouzis Grigorios , Gourgoulianis Konstantinos I.
Published: February 2019

(1) Background: Chronic obstructive pulmonary disease (COPD) is defined as an inflammatory disorder that presents an increasingly prevalent health problem. Accelerated aging has been examined as a pathologic mechanism of many chronic diseases like COPD. We examined whether COPD is combined with accelerated aging, studying two hormones, dehydroepiandrosterone (DHEA) and growth hormone (GH), known to be characteristic biological markers of aging. (2) Methods: Data were collected from 119 participants, 70 (58.8%) COPD patients and 49 (41.2%) from a health control group over the period of 2014–2016 in a spirometry program. Information about their medical history, tobacco use, and blood tests was obtained. (3) Results: The average age of the health control patients was 73.5 years (SD = 5.5), and that of the COPD patients was 75.4 years (SD = 6.9). Both groups were similar in age and sex. A greater proportion of smokers were found in the COPD group (87.1%) versus the control group (36.7%). The majority of COPD patients were classified as STAGE II (51.4%) and STAGE III (37.1%) according to GOLD (Global Initiative for Chronic Obstructive Pulmonary Disease). Levels of DHEA (SD = 17.1) and GH (SD = 0.37) were significantly lower in the COPD group (p < 0.001) compared to those in the controls (SD = 26.3, SD = 0.79). DHEA and GH were more significant and negatively correlated with age. The regression equation of DHEA with age produced a coefficient equal to 1.26. In this study, the difference in DHEA between COPD patients and controls was, on average, 30.2 μg/dL, indicating that the biological age of a COPD patient is on average about 24 years older than that of a control subject of the same age. Similarly, the difference in GH between COPD patients and controls was, on average, 0.42 ng/mL, indicating that the biological age of a COPD patient is on average about 13.1 years older than that of a control subject of the same age. (4) Conclusions: The findings of our study strongly suggest the presence of premature biological aging in COPD patients. Their biological age could actually vary from 13 to 23 years older than non-COPD controls according to DHEA and GH variation.
The Oral NOAEL of Flurochloridone in Male Wistar Rats in Ninety-Day Subchronic Toxicity Test Was 3mg/kg/day
Authors: Zhu Hongyan , Li Rui , Zhou Su , Zhang Suhui , Wang Yu , Liu Shihong , Song Qingwen , Chang Xiuli , Zhang Yubin , Liu Luqing , Tang Liming , Zhou Zhijun
Published: February 2019

A ninety-day toxicity and toxicokinetics of flurochloridone (FLC) were studied in male Wistar rats with oral administration at doses of 3 mg/kg and 10 mg/kg respectively, following the previous study. Apparent toxicity to reproductive system of male rats was still observed at the dose of 10 mg/kg, trace amounts of FLC were still detected 24 hours after administration, testicular weight, epididymal weight and serum testosterone were significantly reduced and sperm abnormalities in epididymis were significantly increased. No abnormalities were found in 3 mg/kg group, it indicated that no-observed-adverse-effect level (NOAEL) of FLC in male rats was 3 mg/kg/day, far below the dose of 20 mg/kg/day reported by European Food Safety Authority (EFSA). Therefore, more attention should be paid to this herbicide.
Denture Wearing Moderates the Association between Aspiration Risk and Incident Pneumonia in Older Nursing Home Residents: A Prospective Cohort Study
Authors: Takeuchi Kenji , Izumi Maya , Furuta Michiko , Takeshita Toru , Shibata Yukie , Kageyama Shinya , Okabe Yuka , Akifusa Sumio , Ganaha Seijun , Yamashita Yoshihisa
Published: February 2019

Aspiration is increasingly recognized as a major risk for pneumonia, but a potential link between wearing dentures and incident pneumonia with aspiration risk is unclear. The aim of this study was to investigate whether denture wearing moderates the association between aspiration risk and incident pneumonia in older adults. We used prospective cohort data of 156 residents aged >70 years from eight nursing homes in Aso, Japan. Aspiration risk was evaluated using the modified water swallowing test. During a 1-year follow-up (2014 to 2015), information on incident pneumonia was obtained from nursing home medical records. During follow-up, pneumonia developed in 7.1% of participants. In the multivariate-adjusted Cox proportional hazards model, after adjusting for potential confounders, aspiration risk was independently associated with a 4.4-fold higher hazard ratio (HR) of incident pneumonia (95% confidence interval, CI, 1.16–16.43). The difference in the risk of incident pneumonia between subjects with aspiration risk who were wearing dentures and those not at risk of aspiration was not significant, whereas those with aspiration risk without dentures had a 7.3-fold higher HR of incident pneumonia than those not at risk of aspiration (95% CI, 1.02–52.63). Denture wearing might partially moderate the increased risk of incident pneumonia associated with aspiration risk.
A Varied Density-based Clustering Approach for Event Detection from Heterogeneous Twitter Data
Authors: Ghaemi Zeinab , Farnaghi Mahdi
Published: February 2019

Extracting the latent knowledge from Twitter by applying spatial clustering on geotagged tweets provides the ability to discover events and their locations. DBSCAN (density-based spatial clustering of applications with noise), which has been widely used to retrieve events from geotagged tweets, cannot efficiently detect clusters when there is significant spatial heterogeneity in the dataset, as it is the case for Twitter data where the distribution of users, as well as the intensity of publishing tweets, varies over the study areas. This study proposes VDCT (Varied Density-based spatial Clustering for Twitter data) algorithm that extracts clusters from geotagged tweets by considering spatial heterogeneity. The algorithm employs exponential spline interpolation to determine different search radiuses for cluster detection. Moreover, in addition to spatial proximity, textual similarities among tweets are also taken into account by the algorithm. In order to examine the efficiency of the algorithm, geotagged tweets collected during a hurricane in the United States were used for event detection. The output clusters of VDCT have been compared to those of DBSCAN. Visual and quantitative comparison of the results proved the feasibility of the proposed method.
MicroRNA29a Reverts the Activated Hepatic Stellate Cells in the Regression of Hepatic Fibrosis through Regulation of ATPase H+ Transporting V1 Subunit C1
Authors: Jing Fei , Geng Yan , Xu Xin-Yi , Xu Hong-Yu , Shi Jin-Song , Xu Zheng-Hong
Published: February 2019

Activated hepatic stellate cells (aHSCs) play a key role in liver fibrosis. During the regression of fibrosis, aHSCs are transformed into inactivated cells (iHSCs), which are quiescent lipid-containing cells and express higher levels of lipid-related genes, such as peroxisome proliferators-activated receptors gamma (PPARγ). Here, we investigated the role of MicroRNA29a (Mir29a) in the resolution of liver fibrosis. Mir29a and lipid-related genes were up-regulated after the recovery of CCl4-induced liver fibrosis in mice. PPARγ agonist rosiglitazone (RSG) promoted de-differentiation of aHSCs to iHSCs and up-regulated MIR29a expression in a human HSC cell line LX-2. MIR29a mimics in vitro promoted the expression of lipid-related genes, while decreased the expression of fibrosis-related genes. MIR29a inhibitor showed the reverse effects. ATPase H+ transporting V1 subunit C1 (Atp6v1c1) was increased in liver fibrosis, while down-regulated after the recovery in mice, and negatively regulated by MIR29a in LX-2 cells. Knockdown of ATP6V1C1 by siRNA decreased alpha-smooth muscle actin (α-SMA) and increased lipid-related genes expression. Simultaneous addition of MIR29a mimics and ATP6V1C1 siRNA further increased RSG promoted expression of lipid-related proteins in vitro. Collectively, MIR29a plays an important role during the trans-differentiation of aHSCs in the resolution of liver fibrosis, in part, through regulation of ATP6V1C1.
Positive Impact of Pulsed Electric Field on Lactic Acid Removal, Demineralization and Membrane Scaling during Acid Whey Electrodialysis
Authors: Dufton Guillaume , Mikhaylin Sergey , Gaaloul Sami , Bazinet Laurent
Published: February 2019

The drying of acid whey is hindered by its high mineral and organic acid contents, and their removal is performed industrially through expensive and environmentally impacting serial processes. Previous works demonstrated the ability to remove these elements by electrodialysis alone but with a major concern—membrane scaling. In this study, two conditions of pulsed electric field (PEF) were tested and compared to conventional DC current condition to evaluate the potential of PEF to mitigate membrane scaling and to affect lactic acid and salt removals. The application of a PEF 25 s/25 s pulse/pause combination at an initial under-limiting current density allowed for decreasing the amount of scaling, the final system electrical resistance by 32%, and the relative energy consumption up to 33%. The use of pulsed current also enabled better lactic acid removal than the DC condition by 10% and 16% for PEF 50 s/10 s and 25 s/25 s, respectively. These results would be due to two mechanisms: (1) the mitigation of concentration polarization phenomenon and (2) the rinsing of the membranes during the pause periods. To the best of our knowledge, this was the first time that PEF current conditions were used on acid whey to both demineralize and deacidify it.
Grain Legumes and Fear of Salt Stress: Focus on Mechanisms and Management Strategies
Authors: Nadeem Muhammad , Li Jiajia , Yahya Muhammad , Wang Minghua , Ali Asif , Cheng Andong , Wang Xiaobo , Ma Chuanxi
Published: February 2019

Salinity is an ever-present major constraint and a major threat to legume crops, particularly in areas with irrigated agriculture. Legumes demonstrate high sensitivity, especially during vegetative and reproductive phases. This review gives an overview of legumes sensitivity to salt stress (SS) and mechanisms to cope with salinity stress under unfavorable conditions. It also focuses on the promising management approaches, i.e., agronomic practices, breeding approaches, and genome editing techniques to improve performance of legumes under SS. Now, the onus is on researchers to comprehend the plants physiological and molecular mechanisms, in addition to various responses as part of their stress tolerance strategy. Due to their ability to fix biological nitrogen, high protein contents, dietary fiber, and essential mineral contents, legumes have become a fascinating group of plants. There is an immense need to develop SS tolerant legume varieties to meet growing demand of protein worldwide. This review covering crucial areas ranging from effects, mechanisms, and management strategies, may elucidate further the ways to develop SS-tolerant varieties and to produce legume crops in unfavorable environments.
Exploration of miRNA and mRNA Profiles in Fresh and Frozen-Thawed Boar Sperm by Transcriptome and Small RNA Sequencing
Authors: Dai Ding-Hui , Qazi Izhar Hyder , Ran Ming-Xia , Liang Kai , Zhang Yan , Zhang Ming , Zhou Guang-Bin , Angel Christiana , Zeng Chang-Jun
Published: February 2019

Due to lower farrowing rate and reduced litter size with frozen-thawed semen, over 90% of artificial insemination (AI) is conducted using liquid stored boar semen. Although substantial progress has been made towards optimizing the cryopreservation protocols for boar sperm, the influencing factors and underlying mechanisms related to cryoinjury and freeze tolerance of boar sperm remain largely unknown. In this study, we report the differential expression of mRNAs and miRNAs between fresh and frozen-thawed boar sperm using high-throughput RNA sequencing. Our results showed that 567 mRNAs and 135 miRNAs were differentially expressed (DE) in fresh and frozen-thawed boar sperm. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the majority of DE mRNAs were enriched in environmental information processing such as cytokine-cytokine receptor interactions, PI3K-Akt signaling, cell adhesion, MAPK, and calcium signaling pathways. Moreover, the targets of DE miRNAs were enriched in significant GO terms such as cell process, protein binding, and response to stimuli. In conclusion, we speculate that DE mRNAs and miRNAs are heavily involved in boar sperm response to environment stimuli, apoptosis, and metabolic activities. The differences in expression also reflect the various structural and functional changes in sperm during cryopreservation.
Cytotoxic Activity of the Histone Deacetylase 3-Selective Inhibitor Pojamide on MDA-MB-231 Triple-Negative Breast Cancer Cells
Authors: Luparello Claudio , Asaro Dalia Maria Lucia , Cruciata Ilenia , Hassell-Hart Storm , Sansook Supojjanee , Spencer John , Caradonna Fabio
Published: February 2019

We examined the effects of the ferrocene-based histone deacetylase-3 inhibitor Pojamide (N1-(2-aminophenyl)-N8-ferrocenyloctanediamide) and its two derivatives N1-(2-aminophenyl)-N6-ferrocenyladipamide and N1-(2-aminophenyl)-N8-ferroceniumoctanediamide tetrafluoroborate on triple-negative MDA-MB-231 breast cancer cells. Viability/growth assays indicated that only the first two compounds at 70 μM concentration caused an approximate halving of cell number after 24 h of exposure, whereas the tetrafluoroborate derivative exerted no effect on cell survival nor proliferation. Flow cytometric and protein blot analyses were performed on cells exposed to both Pojamide and the ferrocenyladipamide derivative to evaluate cell cycle distribution, apoptosis/autophagy modulation, and mitochondrial metabolic state in order to assess the cellular basis of the cytotoxic effect. The data obtained show that the cytotoxic effect of the two deacetylase inhibitors may be ascribed to the onset of non-apoptotic cell death conceivably linked to a down-regulation of autophagic processes and an impairment of mitochondrial function with an increase in intracellular reactive oxygen species. Our work expands the list of autophagy-regulating drugs and also provides a further example of the role played by the inhibition of autophagy in breast cancer cell death. Moreover, the compounds studied may represent attractive and promising targets for subsequent molecular modeling for anti-neoplastic agents in malignant breast cancer.
Tempo-Spatial Pattern of Stepharine Accumulation in Stephania Glabra Morphogenic Tissues
Authors: Gorpenchenko Tatiana Y. , Grigorchuk Valeria P. , Bulgakov Dmitry V. , Tchernoded Galina K. , Bulgakov Victor P.
Published: February 2019

Alkaloids attract great attention due to their valuable therapeutic properties. Stepharine, an aporphine alkaloid of Stephania glabra plants, exhibits anti-aging, anti-hypertensive, and anti-viral effects. The distribution of aporphine alkaloids in cell cultures, as well as whole plants is unknown, which hampers the development of bioengineering strategies toward enhancing their production. The spatial distribution of stepharine in cell culture models, plantlets, and mature micropropagated plants was investigated at the cellular and organ levels. Stepharine biosynthesis was found to be highly spatially and temporally regulated during plant development. We proposed that self-intoxication is the most likely reason for the failure of the induction of alkaloid biosynthesis in cell cultures. During somatic embryo development, the toxic load of alkaloids inside the cells increased. Only specialized cell sites such as vascular tissues with companion cells (VT cells), laticifers, and parenchymal cells with inclusions (PI cells) can tolerate the accumulation of alkaloids, and thus circumvent this restriction. S. glabra plants have adapted to toxic pressure by forming an additional transport secretory (laticifer) system and depository PI cells. Postembryonic growth restricts specialized cell site formation during organ development. Future bioengineering strategies should include cultures enriched in the specific cells identified in this study.
Increased Cytotoxicity of Herpes Simplex Virus Thymidine Kinase Expression in Human Induced Pluripotent Stem Cells
Authors: Iwasawa Chizuru , Tamura Ryota , Sugiura Yuki , Suzuki Sadafumi , Kuzumaki Naoko , Narita Minoru , Suematsu Makoto , Nakamura Masaya , Yoshida Kazunari , Toda Masahiro , Okano Hideyuki , Miyoshi Hiroyuki
Published: February 2019

Human induced pluripotent stem cells (iPSCs) hold enormous promise for regenerative medicine. The major safety concern is the tumorigenicity of transplanted cells derived from iPSCs. A potential solution would be to introduce a suicide gene into iPSCs as a safety switch. The herpes simplex virus type 1 thymidine kinase (HSV-TK) gene, in combination with ganciclovir, is the most widely used enzyme/prodrug suicide system from basic research to clinical applications. In the present study, we attempted to establish human iPSCs that stably expressed HSV-TK with either lentiviral vectors or CRISPR/Cas9-mediated genome editing. However, this task was difficult to achieve, because high-level and/or constitutive expression of HSV-TK resulted in the induction of cell death or silencing of HSV-TK expression. A nucleotide metabolism analysis suggested that excessive accumulation of thymidine triphosphate, caused by HSV-TK expression, resulted in an imbalance in the dNTP pools. This unbalanced state led to DNA synthesis inhibition and cell death in a process similar to a “thymidine block”, but more severe. We also demonstrated that the Tet-inducible system was a feasible solution for overcoming the cytotoxicity of HSV-TK expression. Our results provided a warning against using the HSV-TK gene in human iPSCs, particularly in clinical applications.
Populus euphratica JRL Mediates ABA Response, Ionic and ROS Homeostasis in Arabidopsis under Salt Stress
Authors: Zhang Huilong , Deng Chen , Yao Jun , Zhang Yan-Li , Zhang Yi-Nan , Deng Shurong , Zhao Nan , Sa Gang , Zhou Xiaoyang , Lu Cunfu , Lin Shanzhi , Zhao Rui , Chen Shaoliang
Published: February 2019

Sodium chloride (NaCl) induced expression of a jacalin-related mannose-binding lectin (JRL) gene in leaves, roots, and callus cultures of Populus euphratica (salt-resistant poplar). To explore the mechanism of the PeJRL in salinity tolerance, the full length of PeJRL was cloned from P. euphratica and was transformed into Arabidopsis. PeJRL was localized to the cytoplasm in mesophyll cells. Overexpression of PeJRL in Arabidopsis significantly improved the salt tolerance of transgenic plants, in terms of seed germination, root growth, and electrolyte leakage during seedling establishment. Under NaCl stress, transgenic plants retained K+ and limited the accumulation of Na+. PeJRL-transgenic lines increased Na+ extrusion, which was associated with the upward regulation of SOS1, AHA1, and AHA2 genes encoding plasma membrane Na+/proton (H+) antiporter and H+-pumps. The activated H+-ATPases in PeJRL-overexpressed plants restricted the channel-mediated loss of K+ that was activated by NaCl-induced depolarization. Under salt stress, PeJRL–transgenic Arabidopsis maintained reactive oxygen species (ROS) homeostasis by activating the antioxidant enzymes and reducing the production of O2− through downregulation of NADPH oxidases. Of note, the PeJRL-transgenic Arabidopsis repressed abscisic acid (ABA) biosynthesis, thus reducing the ABA-elicited ROS production and the oxidative damage during the period of salt stress. A schematic model was proposed to show the mediation of PeJRL on ABA response, and ionic and ROS homeostasis under NaCl stress.
Hydroxyethyl Starch 130/0.4 Binds to Neutrophils Impairing Their Chemotaxis through a Mac-1 Dependent Interaction
Authors: Trentini Alessandro , Murganti Francesca , Rosta Valentina , Cervellati Carlo , Manfrinato Maria Cristina , Spadaro Savino , Dallocchio Franco , Volta Carlo Alberto , Bellini Tiziana
Published: February 2019

Several studies showed that hydroxyethyl starch (HES), a synthetic colloid used in volume replacement therapies, interferes with leukocyte-endothelium interactions. Although still unclear, the mechanism seems to involve the inhibition of neutrophils’ integrin. With the aim to provide direct evidence of the binding of HES to neutrophils and to investigate the influence of HES on neutrophil chemotaxis, we isolated and treated the cells with different concentrations of fluorescein-conjugated HES (HES-FITC), with or without different stimuli (N-Formylmethionine-leucyl-phenylalanine, fMLP, or IL-8). HES internalization was evaluated by trypan blue quenching and ammonium chloride treatment. Chemotaxis was evaluated by under-agarose assay after pretreatment of the cells with HES or a balanced saline solution. The integrin interacting with HES was identified by using specific blocking antibodies. Our results showed that HES-FITC binds to the plasma membrane of neutrophils without being internalized. Additionally, the cell-associated fluorescence increased after stimulation of neutrophils with fMLP (p < 0.01) but not IL-8. HES treatment impaired the chemotaxis only towards fMLP, event mainly ascribed to the inhibition of CD-11b (Mac-1 integrin) activity. Therefore, the observed effect mediated by HES should be taken into account during volume replacement therapies. Thus, HES treatment could be advantageous in clinical conditions where a low activation/recruitment of neutrophils may be beneficial, but may be harmful when unimpaired immune functions are mandatory.
From Human Cytogenetics to Human Chromosomics
Authors: Liehr Thomas
Published: February 2019

Background: The concept of “chromosomics” was introduced by Prof. Uwe Claussen in 2005. Herein, the growing insights into human chromosome structure finally lead to a “chromosomic view” of the three-dimensional constitution and plasticity of genes in interphase nuclei are discussed. This review is dedicated to the memory of Prof. Uwe Claussen (30 April 1945–20 July 2008). Recent findings: Chromosomics is the study of chromosomes, their three-dimensional positioning in the interphase nucleus, the consequences from plasticity of chromosomal subregions and gene interactions, the influence of chromatin-modification-mediated events on cells, and even individuals, evolution, and disease. Progress achieved in recent years is summarized, including the detection of chromosome-chromosome-interactions which, if damaged, lead to malfunction and disease. However, chromosomics in the Human Genetics field is not progressing presently, as research interest has shifted from single cell to high throughput, genomic approaches. Conclusion: Chromosomics and its impact were predicted correctly in 2005 by Prof. Claussen. Although some progress was achieved, present reconsiderations of the role of the chromosome and the single cell in Human Genetic research are urgently necessary.