bluuebunny/crossref_metadata_2025 huggingface dataset:
Genomic sequencing has a high diagnostic yield in children with congenital anomalies of the heart and urinary system
Authors: Allred Erika T. , Perens Elliot A. , Coufal Nicole G. , Sanford Kobayashi Erica , Kingsmore Stephen F. , Dimmock David P.
Published: March 2023

Background

Congenital heart defects (CHD) and congenital anomalies of the kidney and urinary tract (CAKUT) account for significant morbidity and mortality in childhood. Dozens of monogenic causes of anomalies in each organ system have been identified. However, even though 30% of CHD patients also have a CAKUT and both organs arise from the lateral mesoderm, there is sparse overlap of the genes implicated in the congenital anomalies for these organ systems. We sought to determine whether patients with both CAKUT and CHD have a monogenic etiology, with the long-term goal of guiding future diagnostic work up and improving outcomes.

Methods

Retrospective review of electronic medical records (EMR), identifying patients admitted to Rady Children's Hospital between January 2015 and July 2020 with both CAKUT and CHD who underwent either whole exome sequencing (WES) or whole genome sequencing (WGS). Data collected included demographics, presenting phenotype, genetic results, and mother's pregnancy history. WGS data was reanalyzed with a specific focus on the CAKUT and CHD phenotype. Genetic results were reviewed to identify causative, candidate, and novel genes for the CAKUT and CHD phenotype. Associated additional structural malformations were identified and categorized.

Results

Thirty-two patients were identified. Eight patients had causative variants for the CAKUT/CHD phenotype, three patients had candidate variants, and three patients had potential novel variants. Five patients had variants in genes not associated with the CAKUT/CHD phenotype, and 13 patients had no variant identified. Of these, eight patients were identified as having possible alternative causes for their CHD/CAKUT phenotype. Eighty-eight percent of all CAKUT/CHD patients had at least one additional organ system with a structural malformation.

Conclusions

Overall, our study demonstrated a high rate of monogenic etiologies in hospitalized patients with both CHD and CAKUT, with a diagnostic rate of 44%. Thus, physicians should have a high suspicion for genetic disease in this population. Together, these data provide valuable information on how to approach acutely ill patients with CAKUT and CHD, including guiding diagnostic work up for associated phenotypes, as well as novel insights into the genetics of CAKUT and CHD overlap syndromes in hospitalized children.
CAFs orchestrates tumor immune microenvironment—A new target in cancer therapy?
Authors: Zhang Chunxue , Fei Yuxiang , Wang Hui , Hu Sheng , Liu Chao , Hu Rong , Du Qianming
Published: March 2023

Cancer immunotherapy has opened a new landscape in cancer treatment, however, the poor specificity and resistance of most targeted therapeutics have limited their therapeutic efficacy. In recent years, the role of CAFs in immune regulation has been increasingly noted as more evidence has been uncovered regarding the link between cancer-associated fibroblasts (CAFs) and the evolutionary process of tumor progression. CAFs interact with immune cells to shape the tumor immune microenvironment (TIME) that favors malignant tumor progression, a crosstalk process that leads to the failure of cancer immunotherapies. In this review, we outline recent advances in the immunosuppressive function of CAFs, highlight the mechanisms of CAFs-immune cell interactions, and discuss current CAF-targeted therapeutic strategies for future study.
Comprehensive analysis of immunogenic cell death associated genes expression, tumor microenvironment, and prognosis in hepatocellular carcinoma
Authors: Xiang Jiankang , Liu Chuan , He Qingmin , He Pengzhan , Dong Weiguo
Published: March 2023

Background: Immunogenic cell death (ICD) plays an important role in the development of cancers. This study attempted to explore the role of ICD in the prognosis of hepatocellular carcinoma (HCC).

Methods: Gene expression and clinical data were downloaded from The Cancer Genome Alas and Gene Expression Omnibus dataset. The immune/stromal/Estimate scores of the tumor microenvironment (TME) were calculated by ESTIMATE and CIBERSORT algorithms. Kaplan-Meier analysis, functional enrichment analysis, least absolute shrinkage and selection operator (LASSO) analysis, and univariate and multivariate Cox regression analysis were used for prognostic gene screening and prognostic model construction. The correlation of immune cell infiltration and risk scores was analyzed as well. Molecular docking was used to explore the relevance of related genes to anti-cancer drugs.

Results: Ten ICD associated differentially expressed genes in HCC were found, and all of them had good predictive ability for HCC. ICD gene high amount of expression group was associated with poor prognosis (p = 0.015). The TME, immune cell infiltration and gene expression were different between ICD high and low groups (all p < 0.05). Six ICD associated genes (BAX, CASP8, IFNB1, LY96, NT5E and PIK3CA) which could predict the survival status were identified and used to construct the prognostic model for HCC. A risk score was calculated and it could be used as an independent prognostic factor in HCC patients (p < 0.001). In addition, the risk score had a positive correlation with macrophage M0 (r = 0.33, p = 0.0086). Molecular docking indicated that sorafenib could bind strongly to the target protein, representing that sorafenib may exert anticancer effects through these six ICD associated genes.

Conclusion: This study established a prognostic model including six ICD associated genes for HCC, which may deepen our understanding of ICD and guide therapy for HCC patients.
Pneumatosis intestinalis post steroid use in a patient with immune-related adverse events: Case report, literature review and FAERS analysis
Authors: Zhang Tingting , Cao Mingnan , Zhao Bin , Pan Chen , Lin Li , Tang Chuanhao , Zhao Zhigang , Duan Jingli , Wang Li , Liang Jun
Published: March 2023

Introduction: The accurate diagnosis of pneumatosis intestinalis (PI) is increasing despite patients’ limited identification of etiologic factors. Recently a patient with lung squamous carcinoma who developed pneumatosis intestinalis following methylprednisolone administration for immune-related adverse events was treated at our hospital. Subsequent a literature review and an analysis of the FDA Adverse Event Reporting System (FAERS) database enabled the identification of additional cases of pneumatosis intestinalis.

Methods: A literature review of the MEDLINE/PubMed and Web of Science Core Collection databases using standard pneumatosis intestinalis search terms to identify published cases of immune checkpoint inhibitors (ICIs) or steroids causing pneumatosis intestinalis were performed. A separate retrospective pharmacovigilance study of FAERS enabled the extraction of unpublished cases of pneumatosis intestinalis between the first quarter of 2005 and the third quarter of 2022. Disproportionality and Bayesian analyses were performed to identify signal detection in reported odds ratios, proportional reporting ratios, information components, and empirical Bayesian geometric means.

Results: Ten case reports of steroid-related pneumatosis intestinalis were retrieved from six published studies. The implicated drug therapies included pre-treatment with steroids before chemotherapy, combination therapy with cytotoxic agents and steroids, and monotherapy with steroids. In the FAERS pharmacovigilance study, 1,272 cases of immune checkpoint inhibitors or steroid-related pneumatosis intestinalis were incidentally reported. The signal detected in five kinds of immune checkpoint inhibitors and six kinds of steroids implied a positive correlation between the drugs and adverse events.

Conclusion: Steroids might be the etiologic factors in the current case of pneumatosis intestinalis. Reports supporting the role of steroids in suspected cases of pneumatosis intestinalis can be found in literature databases and the FAERS database. Even so, as documented in FAERS, immune checkpoint inhibitors-induced pneumatosis intestinalis should not be excluded.
The effect of high oral loading dose of cholecalciferol in non-alcoholic fatty liver disease patients. A randomized placebo controlled trial
Authors: Mohamed Amal Ahmed , Halim Ahmed Abdel , Mohamed Sahar , Mahmoud Seham Mohamed , Bahgat Eldemiry Eman Mohamed , Mohamed Rasha Sobh , Shaheen Mahmoud Maamoun , Naguib Gina G. , Muharram Nashwa M. , Khalil Mona G. , Saed Salma , Ibrahim Randa , Salah Seif Ahmed , Kamal Noha , Nasraldin Karima , Abdelrahman Ali Elsaid , El Borolossy Radwa
Published: March 2023

Background and Aim: Non-alcoholic fatty liver (NAFLD) is one of the most common progressive metabolic disorders worldwide. There are increasing scientific interests nowadays for the association between vitamin D status and Non-alcoholic fatty liver. Earlier studies have revealed that vitamin D deficiency is highly prevalent in Non-alcoholic fatty liver patients that contributes to poor outcomes. Hence, the present study aimed to assess the efficacy and safety of oral cholecalciferol on Non-alcoholic fatty liver patients.

Subjects and Methods: This study was conducted on 140 patients that were randomized either to group 1 that received the standard conventional therapy in addition to placebo or group 2 that received the standard conventional therapy in addition to cholecalciferol during the 4 months study period.

Results: At the end of the study group 2 revealed significant decrease (p < 0.05) in the mean serum level of TG, LDL-C, TC, hsCRP as compared to their baseline results and group 1 results. Additionally, a significant improvement in the serum levels of ALT (p = 0.001) was seen in group 2 at the end of the study when compared to group 1. Whereas group 1 did not show any change in these parameters when compared to group 2 and their baseline results.

Conclusion: Cholecalciferol was shown to have beneficial effects on serum ALT levels, hsCRP levels and lipid profile of NAFLD patients.

Clinical Trial Registration:https://prsinfo.clinicaltrials.gov/prs-users-guide.html, identifier NCT05613192
Genetic architecture of rust resistance in a wheat (Triticum turgidum) diversity panel
Authors: Klymiuk Valentyna , Haile Teketel , Ens Jennifer , Wiebe Krystalee , N’Diaye Amidou , Fatiukha Andrii , Krugman Tamar , Ben-David Roi , Hübner Sariel , Cloutier Sylvie , Pozniak Curtis J.
Published: March 2023

Introduction

Wheat rust diseases are widespread and affect all wheat growing areas around the globe. Breeding strategies focus on incorporating genetic disease resistance. However, pathogens can quickly evolve and overcome the resistance genes deployed in commercial cultivars, creating a constant need for identifying new sources of resistance.

Methods

We have assembled a diverse tetraploid wheat panel comprised of 447 accessions of three Triticum turgidum subspecies and performed a genome-wide association study (GWAS) for resistance to wheat stem, stripe, and leaf rusts. The panel was genotyped with the 90K Wheat iSelect single nucleotide polymorphism (SNP) array and subsequent filtering resulted in a set of 6,410 non-redundant SNP markers with known physical positions.

Results

Population structure and phylogenetic analyses revealed that the diversity panel could be divided into three subpopulations based on phylogenetic/geographic relatedness. Marker-trait associations (MTAs) were detected for two stem rust, two stripe rust and one leaf rust resistance loci. Of them, three MTAs coincide with the known rust resistance genes Sr13, Yr15 and Yr67, while the other two may harbor undescribed resistance genes.

Discussion

The tetraploid wheat diversity panel, developed and characterized herein, captures wide geographic origins, genetic diversity, and evolutionary history since domestication making it a useful community resource for mapping of other agronomically important traits and for conducting evolutionary studies.
Genome-wide association study identifies a gene conferring high physiological phosphorus use efficiency in rice
Authors: Yan Ming , Feng Fangjun , Xu Xiaoyan , Fan Peiqing , Lou Qiaojun , Chen Liang , Zhang Anning , Luo Lijun , Mei Hanwei
Published: March 2023

Phosphate (Pi) is indispensable for the growth and development of plant, and low-Pi stress is a major limitation for crop growth and yield worldwide. The tolerance to low-Pi stress varied among rice germplasm resources. However, the mechanisms underlying the tolerance of rice to low-Pi stress, as a complex quantitative trait, are not clear. We performed a genome-wide association study (GWAS) through a diverse worldwide collection of 191 rice accessions in the field under normal-Pi and low-Pi supply in two years. Twenty and three significant association loci were identified for biomass and grain yield per plant under low-Pi supply respectively. The expression level of OsAAD as a candidate gene from a associated locus was significantly up-regulated after low-Pi stress treatment for five days and tended to return to normal levels after Pi re-supply in shoots. Suppression of OsAAD expression could improve the physiological phosphorus use efficiency (PPUE) and grain yields through affecting the expression of several genes associated with GA biosynthesis and metabolism. OsAAD would be a promising gene for increasing PPUE and grain yield in rice under normal- and low-Pi supply via genome editing.
Cognitive diagnosis and algorithmic cultural analysis of fourth-grade Yi students’ mathematical skills in China: A case study of several primary schools in Puge county, Liangshan prefecture
Authors: Zhang Hong , Ye Shuang , Shi Lingke
Published: March 2023

The Liangshan Yi Autonomous Prefecture is the largest area in China inhabited by the Yi people, and the original Yi characteristics and culture are well maintained. The Yi also have a high degree of ethnic and cultural intermingling with Tibetans, Han and other ethnic groups. The level of mathematical abilities directly determines the quality of mathematical learning of Yi students. Primary four is the stage of “concrete operations,” and is a critical point in the development of mathematical symbolic awareness. In this study, the geographical location of the school and the financial income of the township in which the school is located were used as the basis for sampling, and the DINA model was used to diagnose the mathematical ability of fourth grade students in three rural Yi primary schools in Puge County. The study found that there was individual variability in the mathematical abilities of fourth grade Yi students, with 21 different types of cognitive error patterns identified, the main ones being five. In addition, the state of knowledge of fourth grade Yi students in arithmetic revealed that their overall level of mathematical ability was low, showing a lag, with none of the knowledge attributes of arithmetic being fully mastered. Cultural differences between the Chinese and Yi languages contribute to the difficulties that Yi students have in learning mathematical operations, including differences in understanding the place value system, zero, decimal expressions, and differences in the perception of multiplication and division. The above research can inform the implementation of targeted remediation for teaching and learning.
Happiness and high reliability develop affective trust in in-vehicle agents
Authors: Zieger Scott , Dong Jiayuan , Taylor Skye , Sanford Caitlyn , Jeon Myounghoon
Published: March 2023

The advancement of Conditionally Automated Vehicles (CAVs) requires research into critical factors to achieve an optimal interaction between drivers and vehicles. The present study investigated the impact of driver emotions and in-vehicle agent (IVA) reliability on drivers’ perceptions, trust, perceived workload, situation awareness (SA), and driving performance toward a Level 3 automated vehicle system. Two humanoid robots acted as the in-vehicle intelligent agents to guide and communicate with the drivers during the experiment. Forty-eight college students participated in the driving simulator study. The participants each experienced a 12-min writing task to induce their designated emotion (happy, angry, or neutral) prior to the driving task. Their affective states were measured before the induction, after the induction, and after the experiment by completing an emotion assessment questionnaire. During the driving scenarios, IVAs informed the participants about five upcoming driving events and three of them asked for the participants to take over control. Participants’ SA and takeover driving performance were measured during driving; in addition, participants reported their subjective judgment ratings, trust, and perceived workload (NASA-TLX) toward the Level 3 automated vehicle system after each driving scenario. The results suggested that there was an interaction between emotions and agent reliability contributing to the part of affective trust and the jerk rate in takeover performance. Participants in the happy and high reliability conditions were shown to have a higher affective trust and a lower jerk rate than other emotions in the low reliability condition; however, no significant difference was found in the cognitive trust and other driving performance measures. We suggested that affective trust can be achieved only when both conditions met, including drivers’ happy emotion and high reliability. Happy participants also perceived more physical demand than angry and neutral participants. Our results indicated that trust depends on driver emotional states interacting with reliability of the system, which suggested future research and design should consider the impact of driver emotions and system reliability on automated vehicles.
Exploring the impact of virtual leadership on job satisfaction in the post-COVID-19 era: The mediating role of work–life balance and trust in leaders
Authors: Al Dilby Hala Koleilat , Farmanesh Panteha
Published: March 2023

Leadership remains a highly important role in the management of employees' psychological and physical well-being, particularly in the aftermath of the COVID-19 pandemic. As various sectors adapted virtual settings to overcome the restrictions posed by the pandemic, the vitality of virtual leaders became more pivotal as they could enhance the virtual work environment for employees and steer teams toward organizational goals. This study assessed the effect of virtual leaders on employees' job satisfaction in the information technology sector as a high-performance industry. Furthermore, the mediating effects of trust in leaders and work–life balance on the virtual leadership–job satisfaction relationship were assessed in the proposed model of this research. Through a deductive quantitative approach and using purposive and convenience sampling techniques, a total of 196 respondents participated in the research. The data analysis process was deployed via Smart PLS software and the PLS-SEM technique. The results showed that virtual leaders play a major role in determining information technology (IT) employees' job satisfaction while the mediating effects of both trust in leaders and work–life balance are significant factors that can enhance the work environment for leaders to achieve better results. The statistically significant findings of this research suggest a number of positive work outcomes and pathways with scholarly and managerial implications that can be beneficial for leaders in relevant sectors.
Provision of palliative and end-of-life care in UK care homes during the COVID-19 pandemic: A mixed methods observational study with implications for policy
Authors: Bradshaw Andy , Ostler Sophia , Goodman Claire , Batkovskyte Izabele , Ellis-Smith Clare , Tunnard India , Bone Anna E. , Barclay Stephen , Vernon Martin , Higginson Irene J. , Evans Catherine J. , Sleeman Katherine E.
Published: March 2023

Introduction

Little consideration has been given to how the provision of palliative and end-of-life care in care homes was affected by COVID-19. The aims of this study were to: (i) investigate the response of UK care homes in meeting the rapidly increasing need for palliative and end-of-life care during the COVID-19 pandemic and (ii) propose policy recommendations for strengthening the provision of palliative and end-of-life care within care homes.

Materials and methods

A mixed methods observational study was conducted, which incorporated (i) an online cross-sectional survey of UK care homes and (ii) qualitative interviews with care home practitioners. Participants for the survey were recruited between April and September 2021. Survey participants indicating availability to participate in an interview were recruited using a purposive sampling approach between June and October 2021. Data were integrated through analytic triangulation in which we sought areas of convergence, divergence, and complementarity.

Results

There were 107 responses to the survey and 27 interviews. We found that (i) relationship-centered care is crucial to high-quality palliative and end-of-life care within care homes, but this was disrupted during the pandemic. (ii) Care homes' ability to maintain high-quality relationship-centered care required key “pillars” being in place: integration with external healthcare systems, digital inclusion, and a supported workforce. Inequities within the care home sector meant that in some services these pillars were compromised, and relationship-centered care suffered. (iii) The provision of relationship-centered care was undermined by care home staff feeling that their efforts and expertise in delivering palliative and end-of-life care often went unrecognized/undervalued.

Conclusion

Relationship-centered care is a key component of high-quality palliative and end-of-life care in care homes, but this was disrupted during the COVID-19 pandemic. We identify key policy priorities to equip care homes with the resources, capacity, and expertise needed to deliver palliative and end-of-life care: (i) integration within health and social care systems, (ii) digital inclusivity, (iii) workforce development, (iv) support for care home managers, and (v) addressing (dis)parities of esteem. These policy recommendations inform, extend, and align with policies and initiatives within the UK and internationally.
Combined hearing and vision screening programs: A scoping review
Authors: Oosthuizen Ilze , Frisby Caitlin , Chadha Shelly , Manchaiah Vinaya , Swanepoel De Wet
Published: March 2023

Background and aim

The World Health Organization (WHO) estimates that 1.5 billion and 2.2 billion people have hearing and vision impairment, respectively. The burden of these non-communicable diseases is highest in low- and middle-income countries due to a lack of services and health professionals. The WHO has recommended universal health coverage and integrated service delivery to improve ear and eye care services. This scoping review describes the evidence for combined hearing and vision screening programs.

Method

A keyword search of three electronic databases, namely Scopus, MEDLINE (PubMed), and Web of Science, was conducted, resulting in 219 results. After removing duplicates and screening based on eligibility criteria, data were extracted from 19 included studies. The Joanna Briggs Institute Reviewer Manual and the Preferred Reporting Items for Systematic Reviews and Meta-analyzes (PRISMA) Extension for Scoping Reviews were followed. A narrative synthesis was conducted.

Results

Most studies (63.2%) were from high-income countries, with 31.6% from middle-income and 5.2% from low-income countries. The majority of studies (78.9%) involved children and the four studies reporting on adults all included adults above 50 years of age. Vision screening was most commonly performed with the “Tumbling E” and “Snellen Chart,” while hearing was typically screened using pure tone audiometry. Studies reported referral rates as the most common outcome with sensitivity and specificity rates not reported in any included articles. Reported benefits of combined vision and hearing screenings included earlier detection of vision and hearing difficulties to support functioning and quality of life as well as resource sharing for reduced costs. Challenges to combined screening included ineffective follow-up systems, management of test equipment, and monitoring of screening personnel.

Conclusions

There is limited research evidence for combined hearing and vision screening programs. Although potential benefits are demonstrated, especially for mHealth-supported programs in communities, more feasibility and implementation research are required, particularly in low- and middle-income countries and across all age groups. Developing universal, standardized reporting guidelines for combined sensory screening programs is recommended to enhance the standardization and effectiveness of combined sensory screening programs.
Multilevel analysis of factors for intimate partner violence during pregnancy in Gammo Goffa Zone, South Ethiopia: A community based study
Authors: Utaile Mesfin Mamo , Ahmed Ahmed Ali , Yalew Alemayehu Worku
Published: March 2023

Background

Intimate partner violence during pregnancy is a public health problem that can affect both maternal and fetal life. However, its prevalence and associated factors have not been well studied and understood in Ethiopia. Hence, this study was conducted to assess the individual and community-level factors associated with intimate partner violence during pregnancy in Gammo Goffa Zone, South Ethiopia.

Methods

A community-based cross-sectional study was conducted among 1,535 randomly selected pregnant women from July to October 2020. Data were collected using an interviewer-administered, standardized WHO multi-country study questionnaire and analyzed using STATA 14. A two level mixed-effects logistic regression model was used to identify factors associated with intimate partner violence during pregnancy.

Results

The prevalence of intimate partner violence during pregnancy was found to be 48% (95% CI: 45–50%). Factors affecting violence during pregnancy were identified at the community and individual levels. Access to health facilities (AOR = 0.61; 95% CI: 0.43, 0.85), women feeling isolated from the community (AOR= 1.96; 95% CI: 1.04, 3.69), and strict gender role differences (AOR= 1.45; 95% CI: 1.03, 2.04) were among higher-level factors found to be significantly associated with intimate partner violence during pregnancy. Low decision-making power was found to increase the odds of experiencing IPV during pregnancy (AOR= 2.51; 95% CI: 1.28, 4.92). Similarly, maternal education, maternal occupation, living with the partner's family, current pregnancy intended by the partner, dowry payment, and presence of marital conflict were among the individual- level factors found to increase the odds of experiencing intimate partner violence during pregnancy.

Conclusions

The prevalence of intimate partner violence during pregnancy was high in the study area. Both individual and community-level factors had significant implications on maternal health programs related to violence against women. Socio-demographic and socio-ecological characteristics were identified as associated factors. Since it is a multifaceted problem, special emphasis has to be given to multi-sectoral approaches involving all responsible bodies to mitigate the situation.
Strategy of skull base reconstruction after endoscopic transnasal pituitary adenoma resection
Authors: Zhang Chao , Yang Zhijun , Liu Pinan
Published: March 2023

Objective

Endoscopic endonasal surgery (EES) is commonly performed for resection of lesions of the anterior/middle cranial fossa region. Cerebrospinal fluid (CSF) leakage is a major complication. Skull base reconstruction after EES is challenging. We describe our reconstruction strategy and technique and analyze its outcomes.

Methods

We retrospectively analyzed 703 patients with pituitary adenoma who underwent EES in our center from January 2020 to August 2022. Clinical, imaging, operative, and pathologic data were recorded from the medical records and analyzed. Skull base reconstruction was performed to achieve the following three goals: seal the original leak, eliminate dead space, provide blood supply, and early ambulation. Reconstruction was tailored to individual patients based on grade of CSF leakage encountered during surgery.

Results

The number of patients with a grade 0, 1, 2, and 3 intraoperative CSF leak was 487, 101, 86, and 29, respectively. Overall incidence of postoperative CSF leakage was 0.14% (1/703). Fascia sutured and vascularized nasoseptal flap were selected for all grade 3 CSF leaks. One patient who experienced postoperative CSF leakage developed intracranial infection and were treated with lumbar CSF drainage that failed; eventually re-exploration surgery for repair was required. Other patients did not have complications such as CSF leak and infection. 29 patients with grade 3 CSF leakage did not complain of severe nasal complications after operation. No perioperative complications related to the strategy (overpacking, infections, or hematomas) occurred. Incidence of postoperative CSF leak according to intraoperative leak grade was as follows: grade 0, zero; grade 1, zero; grade 2, 1.16% (1/86); and grade 3, zero.

Conclusion

The principles of sealing the original leak, eliminating dead space, providing blood supply, and early ambulation are key in skull base reconstruction after EES. Individualization of these principles can significantly reduce the incidence of postoperative CSF leakage and intracranial infection and reduce the use of lumbar CSF drainage. Skull base suture technique is safe and effective in patients with high-flow cerebrospinal fluid leaks.
An Iterative Ray Tracing Algorithm to Increase Simulation Speed While Maintaining Overall Precision
Authors: Autio Hanna , Vardaxis Nikolaos-Georgios , Bard Hagberg Delphine
Published: March 2023

Ray tracing is a frequently used method for acoustic simulations, valued for its calculation speed and ease of use. Although it is fast, there are no fully ray tracing-based real-time simulation methods or engines. Under real-time restrictions, ray tracing simulations lose precision and the variance inherent in the random simulation method has too much impact on the outcome. In this paper, an algorithm called iterative ray tracing is presented that reduces the negative effects of real-time restrictions by iteratively improving the initial calculation and increasing the precision over time. In addition, new estimates of the expected value and variance of ray tracing simulations are presented and used to show the iteration steps in the new algorithm reduce variance, while maintaining the expected value. Simulations using iterative ray tracing are compared to measurements and simulations using the classical ray tracing method, and it is shown that iterative ray tracing can be used to improve precision over time. Although more testing is needed, iterative ray tracing can be used to extend most ray tracing algorithms, in order to decrease the adverse effects of real-time restrictions.
The Impact of Rural Households’ Part-Time Farming on Grain Output: Promotion or Inhibition?
Authors: Ge Dongdong , Kang Xiaolan , Liang Xian , Xie Fangting
Published: March 2023

Given the prevalence of part-time farming behaviors in rural households, studying the impact of part-time farming behaviors on grain output is of great practical significance. Using a panel dataset of 5629 Chinese national rural fixed observation point data from 2009 to 2015, this paper used the Propensity Score Matching-Difference in Differences method (PSM-DID) to examine the impact and dynamic effect of rural household’s part-time farming behavior on grain output. In addition, the paper also aims to explore the mechanism of how part-time farming affects grain output by running an OLS in an intermediary effect model. We analyzed the data from two aspects: the effect of rural households’ part-time farming decisions on grain output and the relationship between the income from rural households’ part-time farming and grain output. After accounting for the control variables, the results showed a significant positive correlation between rural households’ part-time farming behavior and grain output, with an influence coefficient of 0.304 tons increase in total grain output. While rural households’ part-time farming behavior inhibited grain output over the short term, it ultimately promoted grain output in the long run. The promotion effect increased with the duration of the rural households’ part-time farming. Moreover, the degree of part-time farming and its impact on grain output are mediated by agricultural labor inputs and agricultural technology inputs. Hence, differences in the employment times of rural households’ part-time farming can lead to varying results in grain output. To improve the efficiency of grain output, this study recommends that the government implement policies that promote orderly rural households’ part-time farming behavior.
Physicochemical and Nutritional Potential of Fifteen Sorghum Cultivars from Burkina Faso
Authors: Bazié David , Dibala Crépin Ibingou , Kondombo Clarisse Pulcherie , Diao Mamounata , Konaté Kiessoun , Sama Hemayoro , Kayodé Adéchola Pierre Polycarpe , Dicko Mamoudou H.
Published: March 2023

(1) Background: Sorghum (Sorghum bicolor (L.) Moench) is a staple food cereal for most of the rural populations in sub-Saharan Africa. In Burkina Faso, a great diversity of sorghum cultivar is cultivated, but its nutritional potential still needs to be assessed. This study aims to characterize the physicochemical and nutritional profile of grains from 15 sorghum cultivars grown in Burkina Faso in order to identify the best ones for selection and breeding programs. (2) Methods: The physicochemical, nutritional, and antioxidant-activity characterizations of the grains were performed according to standard methods. (3) Results: The study shows significant differences between cultivars according to the physicochemical traits of the grains, such as 1000-grain weight, moisture, and germination rate. For nutritional parameters, the best contents of carbohydrates (79.36%), proteins (9.21%), and fats (4.40%) were recorded with cultivars V12, V8, and V11, respectively. The heavy grains are flouriest with high contents of carbohydrates with high ABTS antiradical activity. However, these grains have low contents of proteins and flavonoids. Those with high ash contents are the richest in amylose and phenolic compounds. Principal component analysis based on physicochemical and nutritional characteristics of sorghum grains identified four groups of varieties with specific characteristics. Group 1 (G1), which includes cultivars V1, V12, V13, V14, and V15, is characterized by a high weight of 1000 grains and mealy cultivars with relatively high total carbohydrate content and ABTS.+ antiradical activity. Group 2 (G2) includes cultivars V7, V9, and V11 and is characterized by cultivars with low 1000-grain weight, less floury but good germination rate, high protein, flavonoids, and relatively high antioxidant activity. Group 3 (G3) includes cultivars V3, V4, and V8 with relatively high ash, amylose, and polyphenol contents, while group 4 (G4), which includes cultivars V2 and V6, has high antioxidant activity and high fatty acid content. Conclusions: The study recorded a variation of physicochemical and nutritional characteristics of sorghum grain according to cultivars. The cultivars were divided into four groups. Among them, the group 1 cultivars have the best nutritional traits and could therefore be used in breeding and selection programs to improve the nutritional potential of sorghum.
Evaluation of Growth Conditions, Antioxidant Potential, and Sensory Attributes of Six Diverse Microgreens Species
Authors: Dhaka Ajeet Singh , Dikshit Harsh Kumar , Mishra Gyan P. , Tontang Mechiya Tomuilim , Meena Nand Lal , Kumar Ranjeet Ranjan , Ramesh S. V. , Narwal Sneh , Aski Muraleedhar , Thimmegowda Vinutha , Gupta Sanjeev , Nair Ramakrishnan M. , Praveen Shelly
Published: March 2023

Microgreens belong to a class of functional foods with valuable nutritional elements and diverse health benefits when consumed as food supplements. Its consumption has increased sharply due to the abundance of different health-promoting components than their mature plants. The present study investigated the growth conditions and nutritional profiles of six crops (mungbean, lentil, red radish, pearl-millet, mustard, and red cabbage) as microgreens grown under the light with a 16 h light/8 h dark cycle. Firstly, the optimum temperature and the day of harvesting of each of the microgreens for their maximum yield were standardized. The optimum temperature ranged from 24 to 28 °C, and the best stage for their harvesting ranged from the 6th to 13th day for all six microgreens species. Physiological parameters such as height, yield, color, moisture content, seed weight to fresh weight (FW) ratio, and FW to dry weight (DW) were also estimated. All the microgreens were analyzed for the total phenolics content, total anthocyanin content, vitamin C, free radical scavenging activity, dietary fiber, and phytic acid contents at the harvesting stage. Total phenolics, total anthocyanin, and vitamin C contents ranged from 55 to 1240 mg/100 g, 25 to 186 mg/100 g, and 22 to 86 mg/100 g, respectively, in the studied microgreens. Red cabbage and pearl-millet microgreens accumulated higher phenolics than other studied microgreens, while total anthocyanin content was higher in red radish and pearl-millet microgreens. Vitamin C content was recorded as highest in red cabbage microgreens. DPPH-based free radical scavenging activity ranged from 62–84% and was highest in red cabbage microgreens. All the microgreens were also analyzed for their dietary fiber (DF) content which ranged from 2.5–12.5% and was recorded as maximum in pearl millet microgreens. The findings of this study offer helpful information on the growth circumstances necessary to produce microgreens with the greatest nutrient and health benefits.
The Complete Mitochondrial Genome of Box Tree Moth Cydalima perspectalis and Insights into Phylogenetics in Pyraloidea
Authors: Gao Yichang , Zhang Jie , Wang Qinghao , Liu Qiuning , Tang Boping
Published: March 2023

To resolve and reconstruct phylogenetic relationships within Pyraloidea based on molecular data, the mitochondrial genome (mitogenome) was widely applied to understand phylogenetic relations at different taxonomic levels. In this research, a complete mitogenome of Cydalima perspectalis was recorded, and the phylogenetic position of C. perspectalis was inferred based on the sequence in combination with other available sequence data. According to the research, the circular mitochondrial genome is 15,180 bp in length. It contains 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), 13 typical protein-coding genes (PCGs), and a non-coding control region. The arrangement of a gene of the C. perspectalis mitogenome is not the same as the putative ancestral arthropod mitogenome. All of the PCGs are initiated by ATN codons, except for the cytochrome c oxidase subunit 1 (cox1) gene, which is undertaken by CGA. Five genes have incomplete stop codons that contain only ‘T’. All tRNA genes display a typical clover–leaf structure of mitochondrial tRNA, except for trnS1 (AGN). The control region contained an ‘ATAGG(A)’-like motif followed by a poly-T stretch. Based on the mitochondrial data, phylogenetic analysis within Pyraloidea was carried out using Bayesian inference (BI) and maximum likelihood (ML) analyses. Phylogenetic analysis showed that C. perspectalis is more closely related to Pygospila tyres within Spilomelinae than those of Crambidae and Pyraloidea.
Evaluation of Rumen Degradation Kinetics of Low-Lignin Alfalfa ‘Hi-Gest® 360’ in Saskatchewan Canada
Authors: Damiran Daalkhaijav , Biligetu Bill , Lardner Herbert
Published: March 2023

The objective of this study was to determine rumen degradation kinetics of new low-lignin alfalfa (Medicago sativa L.) cv. Hi-Gest®360 (HiGest) in comparison with conventional alfalfa cv. AC Grazeland (Grazeland) in monoculture and binary mixtures at different maturity stages. Two cultivars of alfalfa (HiGest, and AC Grazeland) and their binary mixtures with hybrid bromegrass (HBG; cv. AC Success), grown in 2019 at two locations (Saskatoon and Lanigan), were cut at three maturity stages of alfalfa (1 = 10% bloom; 2 = 40% bloom; and 3 = 100% bloom). Rumen degradation characteristics, including rapidly degradable fraction (S), potentially degradable fraction (D), undegradable fraction (U), degradation rate (Kd), lag time (T0), and effective degradability (ED) of each component were determined using in situ technique and were analyzed by a first-order kinetic equation described by Ørskov and McDonald with lag time. Generally, in alfalfa monoculture, S or D were decreased and U was increased without affecting Kd and T0, resulting in decreased ED fraction with increasing stage of maturity. In binary mixtures, plant maturity stages have negligible effects on rumen degradation characteristics of CP. HiGest had higher effective degradability of DM (EDDM) as well as of NDF (EDNDF) than Grazeland. In conclusion, HiGest had greater DM and NDF rumen degradation potential relative to Grazeland. HiGest and Grazeland were different in DM and CP degradation patterns, with HiGest having higher EDDM and EDCP than Grazeland.
Defensin Interactions in Relation to Monoclonal and Disease-Related Proteinase 3 Antibodies Binding at the Catalytic Site
Authors: Zoega Morten , Trier Nicole Hartwig , Nejrup Rikke Guldhammer , Chailyan Anna , Friis Tina , Højrup Peter , Houen Gunnar
Published: March 2023

Proteinase 3 (PR3) is a neutrophil granulocyte enzyme and an autoantigen found in several forms of vasculitis. Due to the diagnostic and clinical importance of antibodies (Abs) to PR3, it is important to characterize the protein and the nature of its epitopes. Here, we have characterized PR3 monoclonal antibodies (MAbs) and disease-associated Abs and their dependency on the PR3 structure and modifications, especially interactions with α-defensins. Three MAbs (HYB 172-01, 172-04, 172-05), which bind to PR3 in its native and denatured forms and provide the disulphide bridges, were intact. α-1-antitrypsin (AT) binds to purified human neutrophil granulocyte PR3 and inhibits its proteolytic activity, towards a small synthetic peptide substrate and a large protein substrate (casein). AT also inhibited the binding of the three MAbs to PR3, indicating that they bind in a region affected by AT binding. However, the MAbs did not inhibit PR3 proteolytic activity with a small substrate, showing that they bound at the active site without restricting access to the substrate cleft. Patient-derived Abs showed essentially the same characteristics as the MAbs, with important implications for vasculitis diagnostics and pathophysiology. Current findings illustrate that PR3 epitopes depend on the three-dimensional structure of the PR3/defensin complex, and that the epitopes depend to a smaller or larger degree on PR3/defensin associations.
Update on Therapeutic Drug Monitoring of Beta-Lactam Antibiotics in Critically Ill Patients—A Narrative Review
Authors: Stašek Jan , Keller Filip , Kočí Veronika , Klučka Jozef , Klabusayová Eva , Wiewiorka Ondřej , Strašilová Zuzana , Beňovská Miroslava , Škardová Markéta , Maláska Jan
Published: March 2023

Beta-lactam antibiotics remain one of the most preferred groups of antibiotics in critical care due to their excellent safety profiles and their activity against a wide spectrum of pathogens. The cornerstone of appropriate therapy with beta-lactams is to achieve an adequate plasmatic concentration of a given antibiotic, which is derived primarily from the minimum inhibitory concentration (MIC) of the specific pathogen. In a critically ill patient, the plasmatic levels of drugs could be affected by many significant changes in the patient’s physiology, such as hypoalbuminemia, endothelial dysfunction with the leakage of intravascular fluid into interstitial space and acute kidney injury. Predicting antibiotic concentration from models based on non-critically ill populations may be misleading. Therapeutic drug monitoring (TDM) has been shown to be effective in achieving adequate concentrations of many drugs, including beta-lactam antibiotics. Reliable methods, such as high-performance liquid chromatography, provide the accurate testing of a wide range of beta-lactam antibiotics. Long turnaround times remain the main drawback limiting their widespread use, although progress has been made recently in the implementation of different novel methods of antibiotic testing. However, whether the TDM approach can effectively improve clinically relevant patient outcomes must be proved in future clinical trials.
Efficacy of Penicillin–Streptomycin Brands against Staphylococcus aureus: Concordance between Veterinary Clinicians’ Perception and the Realities
Authors: Tufa Takele Beyene , Guta Asegid , Tufa Tafese B. , Nigussie Dereje , Beyi Ashenafi Feyisa , Gutema Fanta D. , Regassa Fikru
Published: March 2023

Antibiotics must be safe and effective for use in both human and veterinary medicine. However, information about the efficacy of different brands of antibiotics commonly used in veterinary practices is lacking in Ethiopia. In this study, we determined the efficacy of three brands of penicillin–streptomycin (Pen&strep, Penstrep, and Pro&strep) by performing antimicrobial susceptibility testing against Staphylococcus aureus isolated from cow milk from dairy farms in the towns of Sebata and Bishoftu, Central Ethiopia. We also assessed the knowledge, attitudes, and practices (KAP) of veterinarians regarding the quality and use of brand antibiotics and the antibiotic utilization practices of dairy farm personnel using a structured questionnaire. Of 43 S. aureus isolated and tested, 33 (77%), 10 (23%), and 1 (2%) were susceptible to brands A, B, and C, respectively. According to the respondents, all of them reported that penstrep is the most prescribed antibiotic in dairy farms (100%), followed by oxytetracycline (78%) and sulfa drugs (52%). All veterinarians perceived that antibiotics imported from Western countries have a higher efficacy than those from Eastern countries, and they preferred brand A to the other brands, witnessing its better clinical outcome. The majority (87%) and a little more than half (53%) of the respondents perceived the overuse of antibiotics in veterinary clinics and dairy farms, respectively. Our study revealed the better efficacy of brand A against S. aureus compared to the other brands. Interestingly, the veterinarians’ perception of and preference toward the use of brand antibiotics agreed with the findings of our antibacterial susceptibility testing. The prudent use of brand A is critically important for sustaining effective treatment, avoiding the risk of antimicrobial resistance, and helping to address animal welfare issues.