bluuebunny/crossref_metadata_2025 huggingface dataset:
A machine learning based radiomics approach for predicting No. 14v station lymph node metastasis in gastric cancer
Authors: Ma Tingting , Zhao Mengran , Li Xiangli , Song Xiangchao , Wang Lingwei , Ye Zhaoxiang
Published: October 2024

Purpose

To evaluate the potential of radiomics approach for predicting No. 14v station lymph node metastasis (14vM) in gastric cancer (GC).

Methods

The contrast enhanced CT (CECT) images with corresponding clinical information of 288 GC patients were retrospectively collected. Patients were separated into training set (n = 202) and testing set (n = 86). A total of 1,316 radiomics feature were extracted from portal venous phase images of CECT. Seven machine learning (ML) algorithms including naïve Bayes (NB), k-nearest neighbor (KNN), decision tree (DT), logistic regression (LR), random forest (RF), eXtreme gradient boosting (XGBoost) and support vector machine (SVM) were trained for development of optimal radiomics signature. A combined model was established by combining radiomics with important clinicopathological factors. The diagnostic ability of the signature and model were evaluated.

Results

LR algorithm was chosen for signature construction. The radiomics signature exhibited good discrimination accuracy of 14vM with AUCs of 0.83 in the training and 0.77 in the testing set. The risk of 14vM showed significant association with higher radiomics score. A combined model exhibited increased predictive ability and good agreement in the training (AUC = 0.87) and testing (AUC = 0.85) sets.

Conclusion

The ML-based radiomics model provided a promising image biomarker for preoperative detection of 14vM and may help the surgeon to decide whether to add 14v dissection to lymphadenectomy.
Compassionate engagement of communities in support of palliative and end-of-life care: challenges in post-pandemic era
Authors: Vitorino Joel Vieira , Duarte Beatriz Veiga , Ali Amira Mohammed , Laranjeira Carlos
Published: October 2024

Over the years, humanity has faced various global crises of different kinds that have caused great suffering in the community, such as wars, slavery, torture or the Holocaust, but also climate change, economic crises, or sanitary disasters. The recent pandemic posed a barrier to palliative and End-of-Life (EoL) care, as the need for physical distance made it difficult to retain essential human interactions while minimizing the risk of viral transmission. During the COVID-19 pandemic, the robustness of supportive networks (i.e., family, friends, neighbors, and community members) determined whether someone experienced a calm death at home or an unnecessary hospital admission, labeled as an ‘emergency’. In this vein, active establishment and strengthening of such networks are the foundation of compassionate community efforts. Firstly, providing both physical and emotional support to the entire network of caregivers enhances their ability to care for others and improves the overall experience of death, including the process of dying and the ensuing bereavement period. Furthermore, individuals can enhance their own physical and mental health by practicing compassion. The ability of networks to withstand and recover from physical and emotional challenges, while maintaining strong and supportive relationships among its members, depends on the health and overall well-being of those members. Therefore, we argue that active community participation and death education can strengthen a community’s capacity to assist people facing death, dying, and bereavement.
Selection and application of methanol-utilizing bacteria from tomato leaves for biocontrol of gray mold
Authors: Suenaga Hiroyuki , Hira Tomoko , Yoshimura Takahiro , Oka Takuji , Hira Daisuke
Published: October 2024

Gray mold, caused by Botrytis cinerea, is a significant threat to tomato production. Traditional chemical control methods have become increasingly ineffective because of the development of resistance. This study aimed to isolate methanol-utilizing bacteria from tomato leaves and evaluate their biocontrol potential against gray mold. To obtain bacterial suspensions, tomato leaf samples were collected and washed. We analyzed the microbial communities of these samples using 16S rRNA amplicon sequencing and identified several methylotrophic strains. Among these, 405 isolated strains were cultivated on a solid low-nutrient inorganic salt medium containing methanol, and 7 strains exhibiting considerable antifungal activity against B. cinerea were identified. Greenhouse tests revealed that two strains—SY163 and SY183—significantly reduced the severity of gray mold on tomato leaves. Disease index scores and the area under the disease progress curve values confirmed the efficacy of these strains as biocontrol agents. Statistical analysis indicated the effectiveness of pre- and co-application of these strains with B. cinerea. Phylogenetic analysis identified Serratia rubidaea as the inhibitory strain. The biocontrol activity is likely mediated through the production of antifungal compounds and suppression of B. cinerea sporulation. This study provides the basis for developing a technology of gray mold suppression by controlling the abundance of S. rubidaea in plant microbial communities.
Epidemiological dynamics and molecular characterization of HIV drug resistance in eastern China from 2020 to 2023
Authors: Zhu Min , Sun Zhou , Zhang Xingliang , Luo Wenjie , Wu Sisheng , Ye Ling , Xu Ke , Chen Junfang
Published: October 2024

Objective

HIV drug resistance (HIVDR) has become a threat to the elimination of the AIDS epidemic due to the global scale-up of antiretroviral therapy (ART) for HIV-infected individuals. This study aims to investigate the epidemiological dynamics and molecular characterization of HIV pretreatment drug resistance (PDR) and acquired drug resistance (ADR) in Hangzhou, a developed region in China.

Methods

An epidemiological survey combined with a molecular transmission network and Bayesian analysis was conducted. A total of 3,596 individuals with newly confirmed HIV infections (from 2020 to 2023) and 164 individuals with ART failure (from 2021 to 2023) were included. The molecular transmission network was used to identify key drug-resistant transmission clusters, while the Bayesian analysis was utilized to trace the origins and spread of these clusters.

Results

The overall prevalence of PDR was found to be 8.4% (303/3596). Among these cases, PDR to non-nucleoside reverse transcriptase inhibitors (NNRTIs) accounted for 4.7% (170/3596), significantly higher than the resistance observed for protease inhibitors (PIs; 2.8%, p < 0.001) and nucleoside reverse transcriptase inhibitors (NRTIs; 1.4%, p < 0.001). Multivariate logistic regression analysis revealed a significantly higher PDR value among individuals infected with the CRF07_BC subtype compared to those with the CRF08_BC subtype (aOR = 0.56, 95% CI = 0.359–0.859, p = 0.008). The molecular transmission network analysis identified the transmission of the drug resistance mutation (DRM) Q58E within the clusters of the CRF07_BC subtype. The Bayesian analysis suggested that these clusters were introduced into Hangzhou from Shenzhen between 2005 and 2012. Furthermore, the study highlighted 50.6% (83/164) prevalence of ADR among individuals experiencing ART failure. The combined molecular network analysis of virological failure and newly confirmed HIV infections indicated the transmission of the K103N mutation between these groups.

Conclusion

In conclusion, targeted interventions may be necessary for specific subtypes and transmission clusters to control the spread of drug-resistant HIV. Continuous monitoring of resistance patterns is critical to inform treatment strategies and optimize ART regimens.
Analyzing dissemination, quality, and reliability of Chinese brain tumor-related short videos on TikTok and Bilibili: a cross-sectional study
Authors: Zhang Ren , Zhang Zhiwei , Jie Hui , Guo Yi , Liu Yi , Yang Yuan , Li Chuan , Guo Chenglin
Published: October 2024

Background

As the Internet becomes an increasingly vital source of medical information, the quality and reliability of brain tumor-related short videos on platforms such as TikTok and Bilibili have not been adequately evaluated. Therefore, this study aims to assess these aspects and explore the factors influencing the dissemination of such videos.

Methods

A cross-sectional analysis was conducted on the top 100 brain tumor-related short videos from TikTok and Bilibili. The videos were evaluated using the Global Quality Score and the DISCERN reliability instrument. An eXtreme Gradient Boosting algorithm was utilized to predict dissemination outcomes. The videos were also categorized by content type and uploader.

Results

TikTok videos scored relatively higher on both the Global Quality Score (median 2, interquartile range [2, 3] on TikTok vs. median 2, interquartile range [1, 2] on Bilibili, p = 1.51E-04) and the DISCERN reliability instrument (median 15, interquartile range [13, 18.25] on TikTok vs. 13.5, interquartile range [11, 16] on Bilibili, p = 1.66E-04). Subgroup analysis revealed that videos uploaded by professional individuals and institutions had higher quality and reliability compared to those uploaded by non-professional entities. Videos focusing on disease knowledge exhibited the highest quality and reliability compared to other content types. The number of followers emerged as the most important variable in our dissemination prediction model.

Conclusion

The overall quality and reliability of brain tumor-related short videos on TikTok and Bilibili were unsatisfactory and did not significantly influence video dissemination. Future research should expand the scope to better understand the factors driving the dissemination of medical-themed videos.
Cipaglucosidase alfa plus miglustat: linking mechanism of action to clinical outcomes in late-onset Pompe disease
Authors: Byrne Barry J. , Parenti Giancarlo , Schoser Benedikt , van der Ploeg Ans T. , Do Hung , Fox Brian , Goldman Mitchell , Johnson Franklin K. , Kang Jia , Mehta Nickita , Mondick John , Sheikh M. Osman , Sitaraman Das Sheela , Tuske Steven , Brudvig Jon , Weimer Jill M. , Mozaffar Tahseen
Published: October 2024

Enzyme replacement therapy (ERT) is the only approved disease-modifying treatment modality for Pompe disease, a rare, inherited metabolic disorder caused by a deficiency in the acid α-glucosidase (GAA) enzyme that catabolizes lysosomal glycogen. First-generation recombinant human GAA (rhGAA) ERT (alglucosidase alfa) can slow the progressive muscle degeneration characteristic of the disease. Still, most patients experience diminished efficacy over time, possibly because of poor uptake into target tissues. Next-generation ERTs aim to address this problem by increasing bis-phosphorylated high mannose (bis-M6P) N-glycans on rhGAA as these moieties have sufficiently high receptor binding affinity at the resultant low interstitial enzyme concentrations after dosing to drive uptake by the cation-independent mannose 6-phosphate receptor on target cells. However, some approaches introduce bis-M6P onto rhGAA via non-natural linkages that cannot be hydrolyzed by natural human enzymes and thus inhibit the endolysosomal glycan trimming necessary for complete enzyme activation after cell uptake. Furthermore, all rhGAA ERTs face potential inactivation during intravenous delivery (and subsequent non-productive clearance) as GAA is an acid hydrolase that is rapidly denatured in the near-neutral pH of the blood. One new therapy, cipaglucosidase alfa plus miglustat, is hypothesized to address these challenges by combining an enzyme enriched with naturally occurring bis-M6P N-glycans with a small-molecule stabilizer. Here, we investigate this hypothesis by analyzing published and new data related to the mechanism of action of the enzyme and stabilizer molecule. Based on an extensive collection of in vitro, preclinical, and clinical data, we conclude that cipaglucosidase alfa plus miglustat successfully addresses each of these challenges to offer meaningful advantages in terms of pharmacokinetic exposure, target-cell uptake, endolysosomal processing, and clinical benefit.
Effect of supplementation with lutein, zeaxanthin, and omega-3 fatty acids on macular pigment and visual function in young adults with long-term use of digital devices: study protocol for a randomized double-blind placebo-controlled study
Authors: Wang Lina , Ma Mei , Li Yong , Pei Cheng , Wang Jianming , Li Juan , Yang Linjuan , Liu Qianying , Tang Li , Hao Yang , Jiang Huili , Fu Jiaxuan , Xiao Yuyao , Wang Yahui , Cui Meng , Su Tong , Bai Jiaqi , Tang Hao , Wang Yue , Shan Hongying , Jiang Hong , Deng Chaoming , Kong Liyun , Hui Zhaozhao , Ma Le
Published: October 2024

Background

Growing evidence emphasizes the importance of xanthophyll carotenoids and omega-3 fatty acids in eye health. However, the beneficial effects of such supplementation have not been thoroughly discussed among adults with high screen exposure. Current trial evidence on lutein bioavailability is contradictory, and the interactions of dietary intervention with host-related factors remain elusive. This study aims to investigate the comparative effectiveness of supplementation with macular xanthophylls and omega-3 fatty acids on macular pigment optical density (MPOD) and visual function, access the bioavailability of free lutein and lutein ester, and explore the complex interplay between genetic variations, intestinal microbiota, and the dietary intervention in Chinese adults with long-term exposure to digital devices.

Methods

The Lutein, Zeaxanthin, and Omega-3 (LZO) clinical trial is a 24-week multicenter, randomized, double-blind, placebo-controlled trial of 600 participants recruited from research centers, universities, and communities. Individuals are eligible to participate if they are aged over 18 years and use digital devices for over 8 h daily in the last 2 years, and will be randomized to six arms. A total of three visits will be scheduled at baseline, 12 and 24 weeks. The primary outcome is the change in MPOD over the 24-week intervention. The secondary outcomes are changes in visual function (visual acuity, best-corrected visual acuity, contrast and glare sensitivity, critical flicker fusion, reaction time, visuognosis persistence, symptoms and signs of dry eye, retinal thickness, and optical quality), and changes in serum lutein and zeaxanthin concentrations, and erythrocyte membrane omega-3 fatty acids. Genetic variations will be determined using genome-wide genotyping at baseline. 16S rRNA gene sequencing will be utilized to assess microbiome compositional changes before and after intervention.

Discussion

The trial is anticipated to establish early interventions to prevent photochemical ocular damage and delay the onset of vision impairment in young adults with long-term repeated exposure to screen-based electronic devices, and provide valuable insights for the development of precision nutrition strategies for maintaining eye health.

Clinical trial registration

www.clinicaltrials.in.th, Identifier, TCTR20220904002.
The association between empirical dietary inflammatory pattern and risk of cancer and cancer-specific mortality: a systematic review and meta-analysis of prospective cohort studies
Authors: Hosseini Fatemeh S. , Nikparast Ali , Etesami Elahe , Javaheri-Tafti Fatemeh , Asghari Golaleh
Published: October 2024

Background/aim

Current evidence indicates a correlation between the inflammatory potential of diet and the risk of cancer and cancer-specific mortality. This study aimed to assess the association between empirical dietary inflammatory pattern (EDIP), which has recently been designed based on the inflammatory potential of the diet, and the risk of cancer and cancer-specific mortality.

Methods

A systematic literature search was conducted across the PubMed/Medline, Scopus, and Web of Science databases from January 2016 to March 2024. A random effects model was used to calculate the pooled effect size (ES) and 95% confidence intervals (95% CI). Heterogeneity between studies was assessed using the Cochran Q test and the I2 statistic.

Results

From the initial 229 records, 24 prospective cohort studies with 2,683,350 participants and 37,091 cancer incidence cases, as well as 20,819 cancer-specific mortality, were included in our study. Pooled results indicated a significant association between higher adherence to the EDIP and an increased risk of total cancer (ES: 1.10; 95% CI: 1.05–1.15; I2 = 41.1), colorectal cancer (ES: 1.19; 95% CI: 1.11–1.27; I2 = 41.1), and liver cancer (ES: 1.48; 95% CI: 1.14–1.94; I2 = 36.9). However, no significant association between increased adherence to the EDIP and an increased risk of ovarian or endometrial cancer was found. Furthermore, greater adherence to the EDIP was significantly associated with an increased risk of cancer-specific mortality (ES: 1.18; 95% CI: 1.05–1.33; I2 = 45.4).

Conclusion

Our results showed that a diet with higher inflammatory properties is associated with an increased risk of cancer and cancer-specific mortality.

Systematic review registration

PROSPERO registration no. CRD42024496912.
Jiajiejian gel ameliorates thyroid nodules through regulation of thyroid hormones and suppression of the (IL-6, TNF-α, IL-1β)/JAK2/STAT3/VEGF pathway
Authors: Wang Changlin , Gao Xiangju , Qiao Mingqi , Gao Dongmei , Guo Yinghui , Wang Jieqiong , Song Chunhong
Published: October 2024

Background

The high incidence of thyroid nodules and their rapid growth in recent years have become an important issue affecting public health. Traditional Chinese medicine (TCM) external treatments have unique advantages in treating this disease, but the currently available external preparations are relatively few and the therapeutic mechanism is unclear. Jiajiejian gel (JJJG) is a TCM external preparation developed by our team for the thyroid nodule treatment, which has been preliminarily proven to be safe and effective in clinical practice.

Objective

The current study was aimed to elucidate the therapeutic effects and the underlying mechanisms of JJJG on thyroid nodules in rats.

Methods

The contents of paeonol and forsythoside A in JJJG were determined by HPLC. The thyroid nodules rat model was established through oral gavage of 0.1% propylthiouracil (PTU) for 6 weeks and meanwhile the rats were treated with external JJJG (0.26, 0.52, 1.04 g/kg). Subsequently, the therapeutic effect of JJJG was observed by means of ultrasonic examination, morphology observation, organ coefficients determination and histopathological analysis. Mechanismlly, the levels of FT3, FT4 and TSH in serum were measured and transcriptomics methods were used to analyse and screen the key targets and pathways of alleviating thyroid nodules by JJJG. Further, gene and protein expression levels of key factors in the pathways were measured and validated using quantitative real-time PCR, ELISA, western blotting and immunofluorescence, so as to clarify the therapeutic mechanism.

Results

The contents of the paeonol and forsythoside A were 1.160 and 0.608 mg/g, respectively. JJJG reduced thyroid swelling, improved nodular lesions, decreased thyroid coefficients, and inhibited abnormal nodular hyperplasia of follicular epithelial cells. In terms of mechanism, JJJG significantly increased the levels of FT3 and FT4 and decreased TSH level in serum (P < 0.05). Transcriptomics suggested that the (IL-6, TNF-α, IL-1β)/JAK2/STAT3/VEGF pathway may be one of the key mechanisms in the treatment of thyroid nodules by JJJG. Further validation experiments demonstrated that JJJG significantly reduced the mRNA expression and protein content of IL-1β, IL-6 and TNF-α in thyroid tissue, as well as the mRNA expression of JAK2, STAT3 and VEGF and the protein expression of p-JAK2/JAK2, p-STAT3/STAT3 and VEGF (P < 0.05).

Conclusion

This study indicates that JJJG efficiently ameliorates thyroid nodules by regulating the levels of FT3, FT4 and TSH in serum and suppressing (IL-6, TNF-α, IL-1β)/JAK2/STAT3/VEGF pathway in thyroid tissue, providing a potential therapeutic approach for thyroid nodules.
Monocrotaline-mediated autophagy via inhibiting PI3K/AKT/mTOR pathway induces apoptosis in rat hepatocytes
Authors: Guo Yazhou , Yuan Yang , Wang Ruibo , Bai Jun , Jia Yanqing , Qiu Xinxin , Niu Huafeng , Li Long , Luo Yan , Zhao Baoyu , Zhang Zhencang
Published: October 2024

Monocrotaline (MCT), a major pyrrolizidine alkaloid, is well-known for its high liver toxicity. Dysregulation of autophagy induced apoptosis can lead to various liver diseases, including those induced by chemical compounds. Therefore, we aim to explore whether autophagy might serve as a potential strategy for addressing liver apoptosis caused by MCT. In primary rat hepatocytes (PRHs), MCT significantly increased the number of autophagosomes and the expression levels of LC3II, Becline-1, and Atg5, while it decreased the expression of p62 in a concentration-dependent manner at doses of 100, 200, 300, and 400 μM. Western blot assays revealed MCT inhibited the phosphorylation levels of the PI3K/AKT/mTOR pathway. To elucidate the role of autophagy in mediating MCT-induced apoptosis, we further pretreated PRHs with the autophagy agonist Rapamycin and the inhibitors Bafilomycin A1 and Chloroquine, respectively, and assessed the apoptosis of PRHs induced by MCT. The results displayed that Rapamycin increased the apoptosis rate and the expression of cleaved caspase-3, whereas Bafilomycin A1 and Chloroquine reduced the apoptosis and the expression of cleaved caspase-3 in PRHs. This study confirms that autophagy enhances PRHs apoptosis induced by MCT. In summary, this study demonstrates that MCT-induced autophagy via inhibition of the PI3K/AKT/mTOR pathway can lead to apoptosis in PRHs.
Transient changes in L-arginine, asymmetric and symmetric dimethyl arginine in triathletes following Norseman Xtreme Triathlon
Authors: Bonnevie-Svendsen Martin , Nyborg Christoffer , Bratseth Vibeke , Melau Jørgen , Hisdal Jonny
Published: October 2024

Arterial vasodilation is dependent on nitric oxide synthesized from L-arginine by endothelial nitric oxide synthase. Triathletes are reported to display altered serum concentrations of nitric oxide metabolites such as L-arginine, asymmetric dimethyl arginine (ADMA) and symmetric dimethyl arginine (SDMA) shortly after completing long-distance triathlon races. In other populations, similar changes to nitric oxide metabolites are established risk markers of cardiovascular disease. The objective of this study was to assess serum concentrations of metabolites for endothelial nitric oxide synthesis in triathletes one week following a long-distance triathlon race. In this prospective observational study, we used high-performance liquid chromatography to measure circulating concentrations of L-arginine, ADMA, and SDMA in triathletes. Venous blood samples were collected before, immediately after, day one, and one week following the triathlon race. Serum concentrations and L-arginine/ADMA ratio were determined for each time-point and compared to baseline. L-arginine/ADMA ratio was reduced on day one (147 ± 32 vs 163 ± 40, p < 0.02). ADMA was reduced immediately after and increased at day one and remained elevated at one week (0.29 ± 0.05 μM, p < 0.001, 0.44 ± 0.08 μM, p < 0.001 and 0.42 ± 0.07 μM, p = 0.04, respectively vs 0.40 ± 0.05 μM). SDMA was increased at all time-points when compared to baseline (0.48 ± 0.10 μM, p < 0.001, 0.53 ± 0.11 μM, p < 0.001 and 0.42 ± 0.08 μM, p = 0.048 vs 0.38 ± 0.05 μM). L-arginine was only decreased immediately after (46.0 ± 9.3 μM vs. 64.6 ± 16.1 μM, p < 0.001). Long-distance triathlon racing induces altered levels of metabolites for endothelial nitric oxide production that mostly normalizes within one week following racing. The clinical relevance of these transient changes has yet to be elucidated in the athletic population.
Higher fraction of inspired oxygen during anesthesia increase the risk of postoperative pulmonary complications in patients undergoing non-cardiothoracic surgery: a retrospective cohort study
Authors: Wang Tianzhu , Zhao Weixing , Ma Libin , Wu Jing , Ma Xiaojing , Liu Luyu , Cao Jiangbei , Lou Jingsheng , Mi Weidong , Zhang Changsheng
Published: October 2024

Objective

The ideal intra-operative inspired oxygen concentration remains controversial. We aimed to investigate the association between the intraoperative fraction of inspired oxygen (FiO2) and the incidence of postoperative pulmonary complications (PPCs) in patients undergoing non-cardiothoracic surgery.

Methods

This was a retrospective cohort study of elderly patients who underwent non-cardiothoracic surgery between April 2020 and January 2022. According to intraoperative FiO2, patients were divided into low (≤60%) and high (>60%) FiO2 groups. The primary outcome was the incidence of a composite of pulmonary complications (PPCs) within the first seven postoperative days. Propensity score matching (PSM) and inverse probability treatment weighting (IPTW) were conducted to adjust for baseline characteristic differences between the two groups. Multivariate logistic regression analysis was used to calculate the odds ratios (OR) for FiO2 and PPCs.

Results

Among the 3,515 included patients with a median age of 70 years (interquartile range: 68–74), 492 (14%) experienced PPCs within the first 7 postoperative days. Elevated FiO2 was associated with an increased risk of PPCs in all the logistic regression models. The OR of the FiO2 > 60% group was 1.252 (95%CI, 1.015–1.551, P = 0.038) in the univariate analysis. In the multivariate logistic regression models, the ORs of the FiO2 > 60% group were 1.259 (Model 2), 1.314 (Model 3), and 1.32 (model 4). A balanced covariate distribution between the two groups was created using PSM or IPTW. The correlation between elevated FiO2 and an increased risk of PPCs remained statistically significant with PSM analysis (OR, 1.393; 95% CI, 1.077–1.804; P = 0.012) and IPTW analysis (OR, 1.266; 95% CI, 1.086–1.476; P = 0.003).

Conclusion

High intraoperative FiO2 (>60%) was associated with the postoperative occurrence of pulmonary complications, independent of predefined risk factors, in elderly non-cardiothoracic surgery patients. High intraoperative FiO2 should be applied cautiously in surgical patients vulnerable to PPCs.
Molecular mechanism of GIRK2 channel gating modulated by cholesteryl hemisuccinate
Authors: Cui Meng , Lu Yongcheng , Ma Xinyi , Logothetis Diomedes E.
Published: October 2024

Cholesterol, an essential lipid of cell membranes, regulates G protein-gated inwardly rectifying potassium (GIRK) channel activity. Previous studies have shown that cholesterol activates GIRK2 homotetrameric channels, which are expressed in dopaminergic neurons of the brain. Deletion of GIRK2 channels affects both GIRK2 homo- and heterotetrames and can lead to abnormal neuronal excitability, including conditions such as epilepsy and addiction. A 3.5 Å cryo-EM structure of GIRK2 in complex with CHS (cholesteryl hemisuccinate) and PIP2 (phosphatidylinositol 4,5-bisphosphate) has been solved. This structure provides the opportunity to study GIRK2 channel gating dynamics regulated by cholesterol using gating molecular dynamics (GMD) simulations. In the present study, we conducted microsecond-long GMD simulations on the GIRK2 channel in its APO, PIP2, and PIP2/CHS bound states, followed by systematic analysis to gain molecular insights into how CHS modulates GIRK2 channel gating. We found that CHS binding facilitates GIRK2 channel opening, with 43 K+ ion permeation events observed, compared to 0 and 2 K+ ion permeation events for GIRK2-APO and GIRK2/PIP2, respectively. Binding of CHS to the GIRK2 channel enhances PIP2 and channel interactions, which is consistent with previous experimental results. The negatively charged PIP2 alters the internal electrostatic potential field in the channel and lowers the negative free energy barrier for K+ ion permeation.
A feasibility and usability study of a virtual reality tool (VESPA 2.0) for cognitive rehabilitation in patients with mild cognitive impairment: an ecological approach
Authors: Latella Desirèe , Formica Caterina , Ielo Augusto , Grioli Pietro , Marra Angela , Costanzo Daniela , Merlo Maria Emanuele , Pappalardo Salvatore Marco , Corallo Francesco , Marino Silvia , Quartarone Angelo , Calabrò Rocco Salvatore , Maresca Giuseppa
Published: October 2024

Introduction

Mild cognitive impairment (MCI) or “mild neurocognitive disorder” represents an intermediate status between normality and dementia. It is characterized by cognitive decline that does not significantly interfere with normal daily living activities. Virtual reality (VR) is the new frontier of rehabilitation.

Methods

We enrolled 50 MCI patients who underwent a neuropsychological evaluation and participated in 40 sessions of cognitive treatment using the Virtual Environment for a Superior Neuro-Psychiatry, Second Generation (VESPA 2.0) System. This preliminary study highlights the role of VR tools for cognitive rehabilitation (CR) for the recovery of cognitive functions and consequent better management of MCI condition. Our study demonstrated that the VESPA 2.0 System is a valuable tool in a context that closely resembles real-life situations rather than controlled, artificial environments as traditional cognitive training methods.

Results

The results showed that the patient group had significant improvements between T0 and T1 (assessment), in particular, in the global cognitive profile, visuospatial skills, and executive functions after treatment with the VESPA 2.0 System.

Discussion

Our findings contribute with new evidence of understanding the impact of using simulations of the Activities of Daily Living (ADL) scale in the CR.
Pattern and associated factors of COVID-19 knowledge, attitude and practice (KAP) among COVID-19-comorbid patients: a systematic review and meta-analysis
Authors: Rehman Anees ur , Tasleem Zermina , Muhammad Sohail Ayaz , Rasool Muhammad Fawad , Shah Shahid , Jabeen Gul , Arif Sonia , Omar Babkair Lujain Salim , Kadumi Yahya Jaber , Alghamdi Saleh , Almarzoky Abuhussain Safa S. , Khayyat Sarah M. , Alharthi Raed Hamed Hilal , Hossain Mohammad Akbar , Abbas Asma A. , Elrggal Mahmoud Essam , Haseeb Abdul
Published: October 2024

Background

The COVID-19 comorbid population is at higher risk of developing severe health issues like acute respiratory distress syndrome, coagulation syndrome, metabolic acidosis, and septic shock, potentially leading to patient death. Patients’ knowledge, attitudes, and practices (KAP) significantly influence their response to the pandemic and aid in enhancing health policy implementation.

Objective

To identify and evaluate the pattern and associated factors to COVID-19 knowledge, attitude, and practice among individuals with comorbidities.

Methodology

The systematic review followed the PRISMA guidelines. Relevant studies assessing the KAP of comorbid patients were retrieved by carefully searching the PubMed and Google Scholar databases. The appraisal tool for cross-sectional studies was used to determine the quality of the included studies and the risk of biases.

Results

Eighteen studies met the inclusion criteria and were included in the review. The pooled sample size of the included studies was 9,104. Different comorbidities reported in the studies include hypertension, diabetes, psychological disorders, and cancer. Pooled analysis showed that 65% of patients showed good knowledge, 57% of patients showed a positive attitude and 51% of patients followed good practices to manage the COVID-19 in presence of their comorbid condition. Significant factors impacting knowledge, attitude and practice in COVID-19 comorbid patients were ethnicity OR 1.78 [95% CI 1.35–2.32]; educational status 3.2 [2.79–3.58]; urban residence 2.43 [1.65–3.02]; employment Status 1.67[1.34–2.12]; financial Status 4.02[3.66–4.38]; occupation 3.65[3.31–4.25]; information Source 2.64[2.19–3.26]; comorbidity 3.28[2.78–3.61]; and duration of chronic illness 1.59[1.31–2.04].

Conclusion

Comorbid COVID-19 patients showed good knowledge, positive attitude and good practice towards the management of the disease.
Assessment of abdominal obesity prevalence and determinants among adults in southwest Ethiopia: a cross-sectional study
Authors: Yosef Tewodros , Sebeta Asresash , Tekalign Eyob , Sisay Binyam Girma , Bekele Bayu Begashaw , Tesfaw Aragaw , Shifera Nigusie
Published: October 2024

Background

Abdominal obesity, excess fat around the abdomen, is more harmful than other fat types and is a key diagnostic criterion for metabolic syndrome. It poses a growing global public health concern. This study aimed to assess the prevalence of and determinants of abdominal obesity among adults in southwest Ethiopia.

Methods

A cross-sectional study involving 624 adults in Semen Bench district, southwest Ethiopia was conducted from May 23 to June 23, 2022. The data was gathered using questionnaires and anthropometric measurements. The data were analyzed using Stata version 18. A robust Poisson regression was used due to the abdominal obesity prevalence exceeding 10%. Variables with p-values < 0.25 in the bivariate analysis were included in the multivariable analysis. The adjusted prevalence ratio (APR) and 95% confidence interval were reported to indicate statistical significance and the strength of associations.

Results

The prevalence of abdominal obesity was 18% (95% CI: 15–21%). After adjusting for confounding variables, the determinants of abdominal obesity included participants aged 31–50 (APR = 3.62, 95% CI: 2.67–8.95) and 51–65 (APR = 3.24, 95% CI: 2.51–8.69), being female (APR = 2.65, 95% CI: 1.63–4.98), having a low wealth index (APR = 1.46, 95% CI: 1.19–3.76), physical inactivity (APR = 1.39, 95% CI: 1.06–4.18), lack of adequate dietary diversity (APR = 3.08, 95% CI: 2.09–6.42), and food insecurity (APR = 2.84, 95% CI: 1.82–7.68).

Conclusion

The research revealed that 18% of the participants exhibited abdominal obesity. Factors such as advanced age, being female, having a low wealth index, physical inactivity, insufficient dietary diversity, and food insecurity were identified as contributors to abdominal obesity. Therefore, it is crucial to develop targeted interventions to address modifiable factors, as this can also help prevent the onset of non-communicable chronic diseases associated with abdominal obesity.
Deciphering solar magnetic activity: some (unpopular) thoughts on the coupling of the Sun’s “weather” and “climate”
Authors: McIntosh Scott W. , Leamon Robert J.
Published: October 2024

The Sun exhibits episodic surges of magnetic activity across a range of temporal and spatial scales, the most prominent of which is the 11-th year modulation of sunspot production. Beside the ∼170 (min to max) decadal variation in sunspot production there is a less-explored quasi-annual variation in the range of 25–50 sunspots/year in magnitude. In addition, there is there is a slower, ∼80 year period, 10–50 variation in the sunspot number, that is commonly referred to as the “Gleissberg Cycle.” Using a suite of contemporary and historical observations we will illustrate these elements of our star’s episodic behavior and present a hypothesis that may provide a consistent physical link between the observed “climatic,” “decadal,” and “seasonal” magnetic variation of our star.
Training for transformation: examining food systems courses at US land-grant universities
Authors: Hoffs Charlie T , Chharan Nooran , Landry Matthew J. , Ward Catherine P. , Oyewole Kemi A. , Wang May C. , Egan Sophie , Bruce Janine , Gardner Christopher
Published: October 2024

Higher education institutions can play an essential role in preparing students to participate in movements for just and sustainable food systems change. For the past two decades, many United States land-grant universities (LGUs) have developed food systems education (FSE) courses. This study examined the extent to which FSE courses employ four capacities deemed crucial by the FSE literature: multidimensional approaches, interdisciplinarity, centering equity, and training students to take action in food systems. The syllabi of 171 undergraduate courses at 20 LGUs were obtained by contacting instructors, and their course descriptions and learning outcomes were analyzed. This subset of LGUs were identified from the membership list of the Menus of Change University Research Collaborative (MCURC), a nationwide network of colleges using campus dining halls and classrooms as living laboratories for food systems change. Most course descriptions and learning outcomes exhibited multidimensional approaches and interdisciplinarity. However, many failed to incorporate teaching content and practices that help students critically examine equity and social justice issues in food systems, or engage in transformative change. LGUs have both the resources and urgent responsibility to empower students to be part of movements to transform unjust, unsustainable food systems. The findings of this study, and an accompanying open-access syllabus website, aim to accelerate the development of FSE curricula that prepare students to change food systems.
Long-Term Exposure to Nitrogen Dioxide and Ozone and Mortality: Update of the WHO Air Quality Guidelines Systematic Review and Meta-Analysis
Authors: Kasdagli Maria-Iosifina , Orellano Pablo , Pérez Velasco Román , Samoli Evangelia
Published: October 2024

Objectives

We performed a systematic review and meta-analysis on long-term exposure to nitrogen dioxide (NO2) and ozone (O3) with mortality, to expand evidence that informed 2021 the WHO Air Quality Guidelines and guide the Health Risks of Air Pollution in Europe project.

Methods

We included cohorts investigating NO2 and O3 mortality from all-causes, respiratory diseases, chronic obstructive pulmonary disease (COPD), acute lower respiratory infections (ALRI); and NO2 mortality from circulatory, ischemic heart, cerebrovascular diseases and lung cancer. We pooled estimates by random-effects models and investigated heterogeneity. We assessed the certainty of the evidence using the Grading of Recommendations Assessment Development approach and Evaluation (GRADE).

Results

We selected 83 studies for NO2 and 26 for O3 for the meta-analysis. NO2 was associated with all outcomes, except for cerebrovascular mortality. O3 was associated with respiratory mortality following annual exposure. There was high heterogeneity, partly explained by region and pollutant levels. Certainty was high for NO2 with COPD and ALRI, and annual O3 with respiratory mortality.

Conclusion

An increasing body of evidence, with new results from countrywide areas and the Western Pacific, supports certainty, including new outcomes.
Phenological Monitoring of Irrigated Sugarcane Using Google Earth Engine, Time Series, and TIMESAT in the Brazilian Semi-Arid
Authors: Castro Manrique Diego Rosyur , Lopes Pabrício Marcos Oliveira , Nascimento Cristina Rodrigues , Ribeiro Eberson Pessoa , Silva Anderson Santos da
Published: October 2024

Monitoring sugarcane phenology is essential since the globalized market requires reliable information on the quantity of raw materials for the industrial production of sugar and alcohol. In this context, the general objective of this study was to evaluate the phenological seasonality of the sugarcane varieties SP 79-1011 and VAP 90-212 observed from the NDVI time series over 19 years (2001–2020) from global databases. In addition, this research had the following specific objectives: (i) to estimate phenological parameters (Start of Season (SOS), End of Season (EOS), Length of Season (LOS), and Peak of Season (POS)) using TIMESAT software in version 3.3 applied to the NDVI time series over 19 years; (ii) to characterize the land use and land cover obtained from the MapBiomas project; (iii) to analyze rainfall variability; and (iv) to validate the sugarcane harvest date (SP 79-1011). This study was carried out in sugarcane growing areas in Juazeiro, Bahia, Brazil. The results showed that the NDVI time series did not follow the rainfall in the region. The sugarcane areas advanced over the savanna formation (Caatinga), reducing them to remnants along the irrigation channels. The comparison of the observed harvest dates of the SP 79-1011 variety to the values estimated with the TIMESAT software showed an excellent fit of 0.99. The mean absolute error in estimating the sugarcane harvest date was approximately ten days, with a performance index of 0.99 and a correlation coefficient of 0.99, significant at a 5% confidence level. The TIMESAT software was able to estimate the phenological parameters of sugarcane using MODIS sensor images processed on the Google Earth Engine platform during the evaluated period (2001 to 2020).
Research on the Corrosion Resistance and Cytotoxicity of Medical Forged Co-28Cr-6Mo Alloy
Authors: Xu Bo , Xu Yangtao , Wei Jianglong
Published: October 2024

Co-Cr-Mo alloy as a human body implant material has a long history, because of its excellent corrosion resistance and biocompatibility, and is widely used in human hip joint materials. Co-Cr-Mo alloy in the human body is often in a passivation state; the formation of dense oxide film on the alloy surface prevents further corrosion of the alloy. The main component of the passivation film is the oxide of Cr, so a layer of oxide film formed by Cr on the surface of Co-Cr-Mo alloy is the reason for its good corrosion resistance. In biocompatibility, cytotoxicity is the first choice and necessary option for biological evaluation, and cytotoxicity can quickly detect the effect of materials on cells in a relatively short time. Therefore, this research conducted a comparative evaluation on the corrosion resistance and biocompatibility of forged Co-Cr-Mo alloys produced in domestic and foreign alloys in line with medical standards. Three simulated human body fluids and Princeton electrochemical station were selected for corrosion resistance experiments, and it was found that the corrosion resistance of four alloys in sodium citrate solution inside and outside China would be reduced. All the alloys exhibit secondary passivation behavior in Hanks solution, which improves the corrosion resistance of the alloys. According to the self-corrosion potential Ecorr analysis, the corrosion resistance of domestic B alloy is the best, while that of foreign R31537 alloy is poor. In the biocompatibility experiment, the biocompatibility of Co-Cr-Mo alloy was evaluated through the measurement of contact Angle and cytotoxicity reaction. The experimental results show that Co-Cr-Mo alloy is a hydrophilic material, and the contact Angle of foreign R31537 alloy is smaller, indicating that the surface of R31537 alloy is more suitable for cell adhesion and spreading. According to the qualitative and quantitative analysis of the cytotoxicity experiment, the toxic reaction grade of domestic A, B and R31537 alloy is grade 1, the toxic reaction grade of C alloy is grade 2, and C alloy has a slight toxic reaction.
Nitidine Chloride Alleviates Hypoxic Stress via PINK1-Parkin-Mediated Mitophagy in the Mammary Epithelial Cells of Milk Buffalo
Authors: Kong Zhiwei , Pan Haichang , Wang Zi , Abla Alida , Wei Yingming
Published: October 2024

Hypoxia in the mammary gland epithelial cells of milk buffalo (BMECs) can affect milk yield and composition, and it can even cause metabolic diseases. Nitidine chloride (NC) is a natural alkaloid with antioxidant properties that can scavenge excessive reactive oxygen species (ROS). However, the effect of NC on the hypoxic injury of BMECs and its molecular mechanisms are still unknown. Here, an immunofluorescence assay, transmission electron microscopy (TEM), and flow cytometry, combined with untargeted metabolomics, were used to investigate the protective effect of NC on hypoxic stress injury in BMECs. It was found that NC can significantly reduce cell activity (p < 0.05) and inhibit cellular oxidative stress (p < 0.05) and cell apoptosis (p < 0.05). A significant decrease in mitophagy mediated by the PINK1-Parkin pathway was observed after NC pretreatment (p < 0.05). In addition, a metabolic pathway enrichment analysis demonstrated that the mechanisms of NC against hypoxic stress may be related to the downregulation of pathways involving aminoacyl tRNA biosynthesis; arginine and proline metabolism; glycine, serine, and threonine metabolism; phenylalanine, tyrosine, and tryptophan biosynthesis; and phenylalanine metabolism. Thus, NC has a protective effect on hypoxic mitochondria, and it can regulate amino acid metabolism in response to hypoxic stress. The present study provides a reference for the application of nitidine chloride to regulate the mammary lactation function of milk buffalo.
Impact of Nutrient Starvation on Biofilm Formation in Pseudomonas aeruginosa: An Analysis of Growth, Adhesion, and Spatial Distribution
Authors: De Plano Laura Maria , Caratozzolo Manuela , Conoci Sabrina , Guglielmino Salvatore P. P. , Franco Domenico
Published: October 2024

Objectives: This study investigates the impact of nutrient availability on the growth, adhesion, and biofilm formation of Pseudomonas aeruginosa ATCC 27853 under static conditions. Methods: Bacterial behaviour was evaluated in nutrient-rich Luria–Bertani (LB) broth and nutrient-limited M9 media, specifically lacking carbon (M9-C), nitrogen (M9-N), or phosphorus (M9-P). Bacterial adhesion was analysed microscopically during the transition from reversible to irreversible attachment (up to 120 min) and during biofilm production/maturation stages (up to 72 h). Results: Results demonstrated that LB and M9 media supported bacterial growth, whereas nutrient-starved conditions halted growth, with M9-C and M9-N inducing stationary phases and M9-P leading to cell death. Fractal analysis was employed to characterise the spatial distribution and complexity of bacterial adhesion patterns, revealing that nutrient-limited conditions affected both adhesion density and biofilm architecture, particularly in M9-C. In addition, live/dead staining confirmed a higher proportion of dead cells in M9-P over time (at 48 and 72 h). Conclusions: This study highlights how nutrient starvation influences biofilm formation and bacterial dispersion, offering insights into the survival strategies of P. aeruginosa in resource-limited environments. These findings should contribute to a better understanding of biofilm dynamics, with implications for managing biofilm-related infections and industrial biofouling.
Path Planning of Inspection Robot Based on Improved Ant Colony Algorithm
Authors: Wang Haixia , Wang Shihao , Yu Tao
Published: October 2024

The conventional Ant Colony Optimization (ACO) algorithm, applied to logistics robot path planning in a two-dimensional grid environment, encounters several challenges: slow convergence rate, susceptibility to local optima, and an excessive number of turning points in the planned paths. To address these limitations, an improved ant colony algorithm has been developed. First, the heuristic function is enhanced by incorporating artificial potential field (APF) attraction, which introduces the influence of the target point’s attraction on the heuristic function. This modification accelerates convergence and improves the optimization performance of the algorithm. Second, an additional pheromone increment, calculated from the difference in pheromone levels between the best and worst paths of the previous generation, is introduced during the pheromone update process. This adjustment adaptively enhances the path length optimality. Lastly, a triangle pruning method is applied to eliminate unnecessary turning points, reducing the number of turns the logistics robot must execute and ensuring a more direct and efficient path. To validate the effectiveness of the improved algorithm, extensive simulation experiments were conducted in two grid-based environments of varying complexity. Several performance indicators were utilized to compare the conventional ACO algorithm, a previously improved version, and the newly proposed algorithm. MATLAB simulation results demonstrated that the improved ant colony algorithm significantly outperforms the other methods in terms of path length, number of iterations, and the reduction of inflection points, confirming its superiority in logistics robot path planning.