bluuebunny/crossref_metadata_2025 huggingface dataset:
Framework for Operational Resilience Management of Critical Infrastructures and Organizations
Authors: Lichte Daniel , Torres Frank Sill , Engler Evelin
Published: May 2022

Progressing digitalization and networking of systems and organizations representing Critical Infrastructures opens promising new potentials and opportunities, which on the downside, are accompanied by rising complexity and increasingly opaque interdependencies. The consequently increasing lack of knowledge leads to uncertainties affecting risk assessment and decision-making in case of adverse events. This trend motivated recent discussions and developments in risk science, emphasizing the need to handle such uncertainties. Complementarily, research in the resilience domain focuses on system capabilities to handle surprising hazardous situations. Several frameworks presented in the literature aim at combining both perspectives but either lack the focus on operational management, have a rather theoretical approach, or are designed for specific applications. Based on this observation, we propose an approach that integrates resilience management into the actual operation of Critical Infrastructure Systems and Organizations by providing an operational process that coordinates the fundamental resilience capabilities of responding, monitoring, anticipation, and learning. Furthermore, we tackle the challenge of uncertainties resulting from a lack of knowledge by aligning the concepts of digital twin and resilience management. The proposed framework is extensively discussed, and required processes are presented in detail. Eventually, its applicability and potential are reviewed by means of a complex hazardous situation at a Bavarian district heating power plant.
Association between the Right Ventricular Longitudinal Shortening Fraction and Mortality in Acute Respiratory Distress Syndrome Related to COVID-19 Infection: A Prospective Study
Authors: Beyls Christophe , Daumin Camille , Hermida Alexis , Booz Thomas , Ghesquieres Tristan , Crombet Maxime , Martin Nicolas , Huette Pierre , Jounieaux Vincent , Dupont Hervé , Abou-Arab Osama , Mahjoub Yazine
Published: May 2022

Introduction: Right ventricular systolic dysfunction (RVsD) increases acute respiratory distress syndrome mortality in COVID-19 infection (CARDS). The RV longitudinal shortening fraction (RV-LSF) is an angle-independent and automatically calculated speckle-tracking parameter. We explored the association between RV-LSF and 30-day mortality in CARDS patients. Methods: Moderate-to-severe CARDS patients hospitalized at Amiens University Hospital with transesophageal echocardiography performed within 48 h of intensive care unit admission were included. RVsD was defined by an RV-LSF of <20%. The patients were divided into two groups according to the presence of RVsD. Using multivariate Cox regression, clinical and echocardiographic risk factors predicting 30-day mortality were evaluated. Results: Between 28 February 2020 and 1 December 2021, 86 patients were included. A total of 43% (n = 37/86) of the patients showed RVsD and 22% (n = 19/86) of the patients died. RV-LSF was observed in 26 (23.1–29.7)% of the no-RVsD function group and 16.5 (13.7–19.4)% (p < 0.001) of the RVsD group. Cardiogenic shock (n = 7/37 vs. 2/49, p = 0.03) and acute cor pulmonale (n = 18/37 vs. 10/49, p = 0.009) were more frequent in the RVsD group. The 30-day mortality was higher in the RVsD group (15/37 vs. 4/49, p = 0.001). In a multivariable Cox model, RV-LSF was an independent mortality factor (HR 4.45, 95%CI (1.43–13.8), p = 0.01). Conclusion: in a cohort of moderate-to-severe CARDS patients under mechanical ventilation, RVsD defined by the RV-LSF was associated with higher 30-day mortalities.
A Generalistic Approach to Machine-Learning-Supported Task Migration on Real-Time Systems
Authors: Delgadillo Octavio , Blieninger Bernhard , Kuhn Juri , Baumgarten Uwe
Published: May 2022

Consolidating tasks to a smaller number of electronic control units (ECUs) is an important strategy for optimizing costs and resources in the automotive industry. In our research, we aim to enable ECU consolidation by migrating tasks at runtime between different ECUs, which adds redundancy and fail-safety capabilities to the system. In this paper, we present a setup with a generalistic and modular architecture that allows for integrating and testing different ECU architectures and machine learning (ML) models. As part of a holistic testbed, we introduce a collection of reproducible tasks, as well as a toolchain that controls the dynamic migration of tasks depending on ECU status and load. The migration is aided by the machine learning predictions on the schedulability analysis of possible future task distributions. To demonstrate the capabilities of the setup, we show its integration with FreeRTOS-based ECUs and two ML models—a long short-term memory (LSTM) network and a spiking neural network—along with a collection of tasks to distribute among the ECUs. Our approach shows a promising potential for machine-learning-based schedulability analysis and enables a comparison between different ML models.
Evaluating the Shark Deterrent Effects of the Novel Exclusion Barrier in Comparison to the Rigorously Tested Sharksafe Barrier Technology
Authors: O’Connell Craig P , Crews Julia , King Andre , Gressle Juliet
Published: May 2022

Although exceedingly rare, shark attacks have a disproportionately large impact on human behavior, often resulting in shark culls. Due to the invasive nature of shark culls, innovating and testing non-invasive deterrent technologies that may minimize the potential for a rare negative shark encounter has become a conservation priority. One such mitigation approach is barriers, such as exclusion nets and the Sharksafe barrier. With both technologies exhibiting limitations and/or ineffectiveness (e.g., Sharksafe barrier), the development of a more effective technology was warranted. Therefore, this study had two key objectives: (1) to determine if DC 12 Volts 180 Newtons electromagnets can produce deterrent responses in the bull shark (Carcharhinus leucas) and (2) to determine if a newly designed and eco-friendly Exclusion barrier exhibits enhanced C. leucas deterrent capabilities when directly compared to the Sharksafe barrier. Based on 100 baited apparatus trials, electromagnetically treated baits resulted in significantly greater avoidance and reduced feeding frequencies. Furthermore, Poisson generalized linear mixed effect model analyses based on 27, 1-h trials illustrated that the Exclusion barrier region resulted in the greatest avoidance and lowest entrance and exit frequencies when compared to the control and Sharksafe barrier regions. Although the Exclusion barrier did not exclude all interacting sharks, the technology provided superior deterrent efficacy in relation to the Sharksafe barrier. Therefore, with many shark populations exhibiting precipitous declines, continued research on this novel technology on potentially dangerous shark species (e.g., white sharks—Carcharodon carcharias) and in varying ecological conditions (e.g., a high energy coastline) is warranted.
Clinical Aspergillus Signatures in COPD and Bronchiectasis
Authors: Tiew Pei Yee , Thng Kai Xian , Chotirmall Sanjay H.
Published: May 2022

Pulmonary mycoses remain a global threat, causing significant morbidity and mortality. Patients with airways disease, including COPD and bronchiectasis, are at increased risks of pulmonary mycoses and its associated complications. Frequent use of antibiotics and corticosteroids coupled with impaired host defenses predispose patients to fungal colonization and airway persistence, which are associated with negative clinical consequences. Notably, Aspergillus species remain the best-studied fungal pathogen and induce a broad spectrum of clinical manifestations in COPD and bronchiectasis ranging from colonization and sensitization to more invasive disease. Next-generation sequencing (NGS) has gained prominence in the field of respiratory infection, and in some cases is beginning to act as a viable alternative to traditional culture. NGS has revolutionized our understanding of airway microbiota and in particular fungi. In this context, it permits the identification of the previously unculturable, fungal composition, and dynamic change within microbial communities of the airway, including potential roles in chronic respiratory disease. Furthermore, inter-kingdom microbial interactions, including fungi, in conjunction with host immunity have recently been shown to have important clinical roles in COPD and bronchiectasis. In this review, we provide an overview of clinical Aspergillus signatures in COPD and bronchiectasis and cover the current advances in the understanding of the mycobiome in these disease states. The challenges and limitations of NGS will be addressed.
Interactions between Core Elements of the Botrytis cinerea Circadian Clock Are Modulated by Light and Different Protein Domains
Authors: Rojas Vicente , Salinas Francisco , Romero Andrés , Larrondo Luis F. , Canessa Paulo
Published: May 2022

Botrytis cinerea possesses a complex light-sensing system composed of eleven photoreceptors. In B. cinerea, bcwcl1 encodes for the BcWCL1 protein, the orthologue of the blue-light photoreceptor WC-1 from Neurospora crassa. The functional partner of BcWCL1 is the BcWCL2 protein, both interacting in the nucleus and forming the B. cinerea white collar complex (BcWCC). This complex is required for photomorphogenesis and circadian regulation. However, no molecular evidence shows a light-dependent interaction between the BcWCC components or light-sensing capabilities in BcWCL1. In this work, by employing a yeast two-hybrid system that allows for the in vivo analysis of protein–protein interactions, we confirm that BcWCL1 and BcWCL2 interact in the absence of light as well as upon blue-light stimulation, primarily through their PAS (Per-Arnt-Sim) domains. Deletion of the PAS domains present in BcWCL1 (BcWCL1PAS∆) or BcWCL2 (BcWCL2PAS∆) severely impairs the interaction between these proteins. Interestingly, the BcWCL1PAS∆ protein shows a blue-light response and interacts with BcWCL2 or BcWCL2PAS∆ upon light stimulation. Finally, we demonstrate that BcWCL1 and BcWCL1PAS∆ respond to blue light by introducing a point mutation in the photoactive cysteine, confirming that both proteins are capable of light sensing. Altogether, the results revealed the complexity of protein–protein interactions occurring between the core elements of the B. cinerea circadian clock.
Investment Promotion, Tax Competition, and Industrial Land Price in China—Evidence from the Corporate Tax Collection Reform
Authors: Song Huasheng , Sun Guili
Published: May 2022

Fiscal arrangements have significant influences on the use of land in China. The tax collection reform in 2002 stipulates that firms establish after the reform are levied by the state tax bureau while those established before the reform continue to be levied by local tax bureaus. The reform divided similar firms into two groups by the date of establishment and created a discontinuity in the tax enforcement for those firms established around the date of the reform. Based on the land transaction data on a parcel basis, we used the regression discontinuity design to study the impact of reform on industrial land prices. We found that the reform has resulted in significant discontinuities in land prices. The firms levied by the state tax authority receive lower land prices relative to the same type of firms that are levied by local tax authorities. The intuition is that tax collection reform has brought an exogenous constraint on local governments’ ability to engage in tax competition. As a countermeasure, local governments use low-cost land as another way to attract investment.
Effect of sp3–sp2 Transformation on the Wear Rate of the DLC Coating
Authors: Gershman Iosif , Mironov Alexander , Mezrin Alexei , Torskaya Elena , Kuznetsova Tatyana , Lapitskaya Vasilina , Rogachev Alexander
Published: May 2022

Based on the methods of non-equilibrium thermodynamics, it was found that the implementation of spontaneous processes with positive entropy production during friction leads to an increase in the wear intensity. Non-spontaneous processes with negative entropy production lead to a decrease in wear intensity. The tribological characteristics of diamond-like carbon (DLC) with different silicon content were studied. The wear intensity practically does not correlate with the friction coefficient. It is shown that DLC with the highest content of diamond-like inclusions (sp3) in the coating has the highest wear rate. In the same DLC, the most intense sp3–sp2 transformation during friction was observed. The sp3–sp2 transformation is a spontaneous process.
Self-Lubricating Effect of FeWO4 Tribologically Synthesized from WC-(Fe-Mn-C) Composite during High-Speed Sliding against a HSS Disk
Authors: Savchenko Nikolai , Sevostyanova Irina , Tarasov Sergei
Published: May 2022

WC-(Fe-Mn-C) composites with γ-iron and γ + α’ matrices were sintered and then tested at sliding speeds in the range 7–37 m/s. The coefficient of friction was exponentially reduced as a function of sliding speed reaching its minimum at 37 m/s. This behavior was provided by the mechanochemical formation of iron tungstate FeWO4 on the worn surfaces of composite samples. The lubricating effect of iron tungstate did not, however, allow for a reduction in wear. The worn surface was represented by a 3–10 μm-thickness tribological layer composed of fine WC and iron particles cemented by FeWO4. This layer provided the self-lubricating effect in high-speed (high-temperature) sliding because of its easy shear and quasi-viscous behavior. The underlying 25–65 μm of thickness layer was composed of only heat-affected WC and recrystallized iron grains.
Development of Ceramic Tiles from Philippine Nickel Laterite Mine Waste by Ceramic Casting Method
Authors: Bernardo-Arugay Ivyleen C. , Echavez Fel Jane A. , Aquiatan Rae Homer L. , Tabelin Carlito B. , Virtudazo Raymond V. Rivera , Resabal Vannie Joy T.
Published: May 2022

Siltation is the biggest environmental challenge associated with nickel laterite mining in the Philippines. The amount of silt generated is huge and one mitigation strategy currently employed by the mining companies is the construction of siltation ponds where the bulk of the clayey- and silt-sized surface runoffs is collected. However, this poses several serious environmental hazards such as landslides due to heavy rainfall and the potential release of hazardous heavy metals. A promising approach to reduce the risks associated with long-term storage of nickel mine waste (NMW) is to employ circular economy by repurposing it for ceramic applications. While generating useful materials with economic value out of a mine waste, it will result in a reduction in volume of waste for disposal. In this study, the method employed to produce NMW-based ceramic wall and floor tiles is slip casting as it is the most appropriate method in forming tiles with complex surface features. Five formulations of NMW-based slips were made for the casting of ceramic tiles and each slip was characterized for its suitability as raw material. The results of NMW characterization show that NMW could be utilized as raw materials for both ceramic wall and floor tiles and the addition of feldspar can enhance casting and physical properties.
Metallogenic Mechanism of Typical Carbonate-Hosted Uranium Deposits in Guizhou (China)
Authors: Qiu Lin-Fei , Wu Yu , Wang Qiong , Wu Lin-Feng , He Zhong-Bo , Peng Song , Fan Yun-Fei
Published: May 2022

Research on topics such as geological–tectonic evolution, metallogenic models of deposits (gold, mercury, lead, zinc, etc.), and ore-forming fluids’ evolution has been conducted in Guizhou. However, few studies have been conducted on uranium (U) deposits (especially carbonate-hosted U deposits). Moreover, the relationship between hydrocarbon fluids and U-mineralization has not been addressed at all. Typical carbonate-hosted U deposits (including some ore spots) in Guizhou Province have been investigated through close field work, petrography, mineralogical, micro-spectroscopy, organic geochemical and C isotope studies. The central part of the U-ore body is often black (the black alternation zone) at the outcrop, and its sides are gray and gray-brown (the gray alternation zone); the color gradually becomes lighter (black to gray) from the center of the ore body out to the sides. Petrographic observations, microscopic laser Raman spectroscopy, and infrared spectroscopic and scanning electron microscope analyses have indicated that U-minerals (pitchblende and coffinite), pyrite and “black” organic matter (OM) are closely co-dependent, with the OM having the typical characteristics of bitumen. Large light oil fluid inclusions were found in gray alternation rocks (besides the U-ore body) with strong light blue fluorescence properties, indicating that hydrocarbon fluids and U-minerals may came from the same U-bearing hydrocarbon fluids. The values of the 13C isotope value, a biomarker of OM and trace elements, REEs in U-ores, were found to be similar here to those in the local paleo-petroleum reservoir, indicating that the bitumen may originate from the deeply intruding paleo-petroleum reservoir. The precipitation of U is related to the cracking differentiation of hydrocarbon fluids. As result, the carbonate-hosted U-mineralization in Guizhou is neither of a sedimentary diagenesis type, nor of a sedimentary diagenesis superimposed leaching hydrothermal transformation type, as have been described by previous scholars. To be exact, the U deposit is controlled by fault and hydrocarbon fluids, and so it can be defined as a structural hydrocarbon–carbonate-type U deposit. A new U-mineralization model was proposed in this study. Here, U, molybdenum, and other metals were mainly found in the black rocks in the lower stratum (presumably Niutitang Formation), having migrated together with hydrocarbon fluids in the form of tiny mineral inclusions. The hydrocarbon fluids (containing some brine) caused cracking and differentiation upon entering the fracture zone, at which point the ore-forming materials (U, pyrite, and other metals) were released and precipitated.
Nanoformulation of Polyphenol Curcumin Enhances Cisplatin-Induced Apoptosis in Drug-Resistant MDA-MB-231 Breast Cancer Cells
Authors: Karami Parastoo , Othman Goran , Housein Zjwan , Salihi Abbas , Hosseinpour Feizi Mohammad Ali , Azeez Hewa Jalal , Babaei Esmaeil
Published: May 2022

Triple Negative Breast Cancer (TNBC) is the aggressive and lethal type of breast malignancy that develops resistance to current therapies. Combination therapy has proven to be an effective strategy on TNBC. We aimed to study whether the nano-formulation of polyphenolic curcumin (Gemini-Cur) would affect the cisplatin-induced toxicity in MDA-MB-231 breast cancer cells. MDA-MB-231 cells were treated with Gemini-Cur, cisplatin and combination of Gemini-Cur/Cisplatin in a time- and dose-dependent manner. Cell viability was studied by using MTT, fluorescence microscopy and cell cycle assays. The mode of death was also determined by Hoechst staining and annexin V-FITC. Real-time PCR and western blotting were employed to detect the expression of BAX and BCL-2 genes. Our data demonstrated that Gemini-Cur significantly sensitizes cancer cells to cisplatin (combination index ≤ 1) and decreases IC50 values in comparison with Gemini-cur or cisplatin. Further studies confirmed that Gemini-Cur/Cisplatin suppresses cancer cell growth through induction of apoptosis (p < 0.001). In conclusion, the data confirm the synergistic effect of polyphenolic curcumin on cisplatin toxicity and provide attractive strategy to attain its apoptotic effect on TNBC.
Analysis of Plant–Plant Interactions Reveals the Presence of Potent Antileukemic Compounds
Authors: Mery David E. , Compadre Amanda J. , Ordóñez Paola E. , Selvik Edward J. , Morocho Vladimir , Contreras Jorge , Malagón Omar , Jones Darin E. , Breen Philip J. , Balick Michael J. , Gaudio Flavio G. , Guzman Monica L. , Compadre Cesar M.
Published: May 2022

A method to identify anticancer compounds in plants was proposed based on the hypothesis that these compounds are primarily present in plants to provide them with an ecological advantage over neighboring plants and other competitors. According to this view, identifying plants that contain compounds that inhibit or interfere with the development of other plant species may facilitate the discovery of novel anticancer agents. The method was developed and tested using Magnolia grandiflora, Gynoxys verrucosa, Picradeniopsis oppositifolia, and Hedyosmum racemosum, which are plant species known to possess compounds with cytotoxic activities. Plant extracts were screened for growth inhibitory activity, and then a thin-layer chromatography bioautography assay was conducted. This located the major antileukemic compounds 1, 2, 4, and 5 in the extracts. Once the active compounds were located, they were extracted and purified, and their structures were determined. The growth inhibitory activity of the purified compounds showed a significant correlation with their antileukemic activity. The proposed approach is rapid, inexpensive, and can easily be implemented in areas of the world with high biodiversity but with less access to advanced facilities and biological assays.
Preparation and Photocatalytic Performance of TiO2 Nanowire-Based Self-Supported Hybrid Membranes
Authors: Shehab Mohammed Ahmed , Sharma Nikita , Valsesia Andrea , Karacs Gábor , Kristály Ferenc , Koós Tamás , Leskó Anett Katalin , Nánai Lilla , Hernadi Klara , Németh Zoltán
Published: May 2022

Nowadays, the use of hybrid structures and multi-component materials is gaining ground in the fields of environmental protection, water treatment and removal of organic pollutants. This study describes promising, cheap and photoactive self-supported hybrid membranes as a possible solution for wastewater treatment applications. In the course of this research work, the photocatalytic performance of titania nanowire (TiO2 NW)-based hybrid membranes in the adsorption and degradation of methylene blue (MB) under UV irradiation was investigated. Characterization techniques such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDS), X-ray powder diffractometry (XRD) were used to study the morphology and surface of the as-prepared hybrid membranes. We tested the photocatalytic efficiency of the as-prepared membranes in decomposing methylene blue (MB) under UV light irradiation. The hybrid membranes achieved the removal of MB with a degradation efficiency of 90% in 60 min. The high efficiency can be attributed to the presence of binary components in the membrane that enhanced both the adsorption capability and the photocatalytic ability of the membranes. The results obtained suggest that multicomponent hybrid membranes could be promising candidates for future photocatalysis-based water treatment technologies that also take into account the principles of circular economy.
Characterizing the ZrC(111)/c-ZrO2(111) Hetero-Ceramic Interface: First Principles DFT and Atomistic Thermodynamic Modeling
Authors: Osei-Agyemang Eric , Paul Jean-François , Lucas Romain , Foucaud Sylvie , Cristol Sylvain , Mamede Anne-Sophie , Nuns Nicolas , Addad Ahmed
Published: May 2022

The mechanical and physical properties of zirconium carbide (ZrC) are limited to its ability to deteriorate in oxidizing environments. Low refractory oxides are typically formed as layers on ZrC surfaces when exposed to the slightest concentrations of oxygen. However, this carbide has a wide range of applications in nuclear reactor lines and nozzle flaps in the aerospace industry, just to name a few. To develop mechanically strong and oxygen-resistant ZrC materials, the need for studying and characterizing the oxidized layers, with emphasis on the interfacial structure between ZrC and the oxidized phases, cannot be understated. In this paper, the ZrC(111)//c-ZrO2 (111) interface was studied by both finite temperature molecular dynamic simulation and DFT. The interfacial mechanical properties were characterized by the work of adhesion which revealed a Zr|OO|Zr|OO//ZrC(111) interface model as the most stable with an oxygen layer from ZrO2 being deposited on the ZrC(111) surface. Further structural analysis at the interface showed a crack in the first ZrO2 layer at the interfacial region. Investigations of the electronic structure using the density of state calculations and Bader charge analysis revealed the interfacial properties as local effects with no significant impacts in the bulk regions of the interface slab.
Harnessing Greenhouse Gases Absorption by Doped Fullerenes with Externally Oriented Electric Field
Authors: Lemos Silva Rodrigo A. , Scalabrini Machado Daniel F. , Nunes Rodrigues Núbia Maria , de Oliveira Heibbe C. B. , Ribeiro Luciano , da Silva Filho Demétrio A.
Published: May 2022

In this work, a theoretical investigation of the effects caused by the doping of C20 with silicon (Si) atom as well as the adsorption of CO, CO2 and N2 gases to C20 and C19Si fullerenes was carried out. In concordance with previous studies, it was found that the choice of the doping site can control the structural, electronic, and energetic characteristics of the C19Si system. The ability of C20 and C19Si to adsorb CO, CO2 and N2 gas molecules was evaluated. In order to modulate the process of adsorption of these chemical species to C19Si, an externally oriented electric field was included in the theoretical calculations. It was observed that C19Si is highly selective with respect to CO adsorption. Upon the increase of the electric field intensity the adsorption energy was magnified correspondingly and that the interaction between CO and C19Si changes in nature from a physical adsorption to a partial covalent character interaction.
Brain Melody Interaction: Understanding Effects of Music on Cerebral Hemodynamic Responses
Authors: Rahman Jessica Sharmin , Caldwell Sabrina , Jones Richard , Gedeon Tom
Published: May 2022

Music elicits strong emotional reactions in people, regardless of their gender, age or cultural background. Understanding the effects of music on brain activity can enhance existing music therapy techniques and lead to improvements in various medical and affective computing research. We explore the effects of three different music genres on people’s cerebral hemodynamic responses. Functional near-infrared spectroscopy (fNIRS) signals were collected from 27 participants while they listened to 12 different pieces of music. The signals were pre-processed to reflect oxyhemoglobin (HbO2) and deoxyhemoglobin (HbR) concentrations in the brain. K-nearest neighbor (KNN), random forest (RF) and a one-dimensional (1D) convolutional neural network (CNN) were used to classify the signals using music genre and subjective responses provided by the participants as labels. Results from this study show that the highest accuracy in distinguishing three music genres was achieved by deep learning models (73.4% accuracy in music genre classification and 80.5% accuracy when predicting participants’ subjective rating of emotional content of music). This study validates a strong motivation for using fNIRS signals to detect people’s emotional state while listening to music. It could also be beneficial in giving personalised music recommendations based on people’s brain activity to improve their emotional well-being.
Innovative Nanomaterials for Biomedical Applications
Authors: Bianchi Michele , Carnevale Gianluca
Published: May 2022

Research focusing on innovative nanomaterials for applications in biomedicine and bioengineering has steadily gained attention over the last 20 years [...]
Bright Single-Photon Sources for the Telecommunication O-Band Based on an InAs Quantum Dot with (In)GaAs Asymmetric Barriers in a Photonic Nanoantenna
Authors: Rakhlin Maxim , Klimko Grigorii , Sorokin Sergey , Kulagina Marina , Zadiranov Yurii , Kazanov Dmitrii , Shubina Tatiana , Ivanov Sergey , Toropov Alexey
Published: May 2022

We report on single-photon emitters for the telecommunication O-band (1260–1360 nm), which comprise an InAs/(In)GaAs quantum dot with asymmetric barriers, placed inside a semiconductor tapered nanocolumn acting as a photonic nanoantenna. The implemented design of the barriers provides a shift in the quantum dot radiation wavelength towards the O-band, while the nanoantenna collects the radiation and ensures its effective output. With non-resonant optical pumping, the average count rate of emitted single photons exceeds 10 MHz with the second-order correlation function g(2)(0) = 0.18 at 8 K.
A Structure—Activity Relationship Study of the Inhibition of α-Amylase by Benzoic Acid and Its Derivatives
Authors: Guan Lei , Long Haoyuan , Ren Fazheng , Li Yixuan , Zhang Hao
Published: May 2022

Phenolic acids are widely found in fruits and vegetables. The inhibitory effect of phenolic acids on α-amylase, a key enzyme for starch digestion, has attracted the attention of researchers. To further investigate the effects of different substituents on the benzene ring of phenolic acid on the inhibition of α-amylase activity, in vitro experiments and molecular docking were used. The structure-activity relationships of 17 phenolic acids with benzoic acid as the parent nucleus were analyzed by determining their half inhibitory concentration (IC50) toward α-amylase. The results showed that 2,3,4-trihydroxybenzoic acid had the strongest inhibitory effect on α-amylase with an IC50 value of 17.30 ± 0.73 mM. According to the structure-activity analysis, the hydroxyl group at the 2-position on the benzene ring had a strong positive effect on the inhibitory activity of α-amylase, while methoxylation at the 2-position and hydroxylation at the 5-position had a negative effect. Molecular docking revealed that hydrogen bonding and hydrophobic interactions were involved in the inhibition, with hydrogen bonding being the primary force. These findings provide a more comprehensive understanding of phenolic acids as inhibitors of α-amylase and provide new ideas for the design of dietary formulations for diabetic patients.
Nutritional Status in Chinese Patients with Obesity Following Sleeve Gastrectomy/Roux-en-Y Gastric Bypass: A Retrospective Multicenter Cohort Study
Authors: Zhang Chunlan , Chen Xi , Liu Shiping , Liu Wei , Zhu Dalong , Li Xiaoying , Qu Shen , Zhu Zhiming , Zhang Jingjing , Zhou Zhiguang
Published: May 2022

Metabolic surgery (MS) is one of the most effective therapies for treating obesity. Due to the lack of multicenter cohort research on nutritional evaluations after surgery in Chinese patients, we explored the changes in nutritional status following MS in Chinese patients. This was a retrospective study of patients (n = 903) who underwent sleeve gastrectomy (SG) (n = 640) or Roux-en-Y gastric bypass (RYGB) (n = 263) for obesity at five different hospitals in China between 17 February 2011, and 20 December 2019. Major nutrients were evaluated at baseline and 1, 3, 6, and 12 months postoperatively. Hb levels decreased, and anemia prevalence increased at 12 months after MS in the premenopausal female group. Moreover, patients with preoperative anemia had an increased risk of postoperative anemia. The ferritin levels (p < 0.001) decreased and iron deficiency increased (p < 0.001) at 12 months after MS among premenopausal females. No significant changes in folate deficiency and vitamin B12 deficiency were found throughout the study. The bone mineral density (BMD) of the femoral neck, lumbar spine, and total hip significantly decreased from baseline to 12 months after MS; however, no new patients developed osteopenia or osteoporosis after MS. Based on 12 months of follow-up, premenopausal females presented a high incidence of anemia after MS. Although we found no differences in osteopenia and osteoporosis prevalence after MS, the BMD did decrease significantly, which suggests that nutrient supplements and long-term follow-up are especially necessary postoperation.
Vitamin B12 Levels, Substance Use Patterns and Clinical Characteristics among People with Severe Substance Use Disorders: A Cohort Study from Western Norway
Authors: Madebo Tesfaye , Bemanian Mitra , Vold Jørn Henrik , Chowdhury Ranadip , Aas Christer Frode , Druckrey-Fiskaaen Karl Trygve , Johansson Kjell Arne , Fadnes Lars Thore
Published: May 2022

People with severe substance use disorder (SUD) have a higher burden of micronutrient deficiency compared with the general population. The aim of this study was to investigate vitamin B12 status and risk factors of deficiency related to substance use, opioid agonist therapy (OAT), as well as hepatitis C infection and liver fibrosis. In this prospective cohort study, participants were recruited from outpatient OAT and SUD clinics in western Norway, and assessed annually with a clinical interview and exam, including venous blood sampling. Data were collected between March 2016 and June 2020, and a total of 2451 serum vitamin B12 measurements from 672 participants were included. The median serum vitamin B12 concentration was 396 (standard deviation 198) pmol/L at baseline, 22% of the population had suboptimal levels (<300 pmol/L) and 1.2% were deficient at baseline (<175 pmol/L). No clear associations were seen with substance use patterns, but liver disease and younger age were associated with higher vitamin B12 levels. Although the majority of participants had satisfactory vitamin B12 levels, about a fifth had suboptimal levels that might or might not be adequate for metabolic needs. Future studies could investigate potential gains in interventions among patients with suboptimal but non-deficient levels.
Qualified Nurses’ Perceptions of Cultural Competence and Experiences of Caring for Culturally Diverse Patients: A Qualitative Study in Four European Countries
Authors: Antón-Solanas Isabel , Rodríguez-Roca Beatriz , Vanceulebroeck Valérie , Kömürcü Nuran , Kalkan Indrani , Tambo-Lizalde Elena , Huércanos-Esparza Isabel , Casa Nova Antonio , Hamam-Alcober Nadia , Coelho Margarida , Coelho Teresa , Van Gils Yannic , Öz Seda Degirmenci , Kavala Arzu , Subirón-Valera Ana B.
Published: May 2022

Background: European nurses are expected to provide appropriate care for patients from diverse cultural backgrounds. However, there is limited knowledge and understanding of this process. The aim of this study was to analyse the perceptions of culture and experiences of caring for patients from diverse cultural backgrounds of a purposive sample of qualified nurses from four European countries, namely Belgium, Portugal, Spain and Turkey. Methods: A qualitative phenomenological approach was selected in order to understand complex phenomena through the participants’ lived experiences, meanings and perspectives. Individual interviews and focus groups took place with 28 staff nurses and 11 nurse managers from four European countries. The sociodemographic and cultural characteristics of the sample were described and analysed using descriptive statistics. Qualitative data were transcribed verbatim, translated into English and analysed following Braun and Clark’s phases for thematic analysis. Results: Five themes and twelve subthemes emerged from thematic analysis of the transcripts. The themes included: (1) relevance of culture for nursing; (2) culture in the healthcare service; (3) qualities of the healthcare professionals; (4) challenges to culturally competent care; (5) becoming a culturally competent nurse. Conclusions: There are challenges to the delivery of culturally congruent care, namely language and communication difficulties, prejudices and stereotyping in the health service, a tendency for ethnocentrism, a lack of education and training in cultural competence and a lack of support from the health service to facilitate new ways of acting.
Oral Administration of Valganciclovir Reduces Clinical Signs, Virus Shedding and Cell-Associated Viremia in Ponies Experimentally Infected with the Equid Herpesvirus-1 C2254 Variant
Authors: Thieulent Côme J. , Sutton Gabrielle , Toquet Marie-Pierre , Fremaux Samuel , Hue Erika , Fortier Christine , Pléau Alexis , Deslis Alain , Abrioux Stéphane , Guitton Edouard , Pronost Stéphane , Paillot Romain
Published: May 2022

Equid alphaherpesvirus-1 (EHV-1) is one of the main pathogens in horses, responsible for respiratory diseases, ocular diseases, abortions, neonatal foal death and neurological complications such as equine herpesvirus myeloencephalopathy (EHM). Current vaccines reduce the excretion and dissemination of the virus and, therefore, the extent of an epizooty. While their efficacy against EHV-1-induced abortion in pregnant mares and the decreased occurrence of an abortion storm in the field have been reported, their potential efficacy against the neurological form of disease remains undocumented. No antiviral treatment against EHV-1 is marketed and recommended to date. This study aimed to measure the protection induced by valganciclovir (VGCV), the prodrug of ganciclovir, in Welsh mountain ponies experimentally infected with an EHV-1 ORF30-C2254 strain. Four ponies were administered VGCV immediately prior to experimental EHV-1 infection, while another four ponies received a placebo. The treatment consisted in 6.5 mg/kg body weight of valganciclovir administered orally three times the first day and twice daily for 13 days. Clinical signs of disease, virus shedding and viraemia were measured for up to 3 weeks. The severity of the cumulative clinical score was significantly reduced in the treated group when compared with the control group. Shedding of infectious EHV-1 was significantly reduced in the treated group when compared with the control group between Day + 1 (D + 1) and D + 12. Viraemia was significantly reduced in the treated group when compared with the control group. Seroconversion was measured in all the ponies included in the study, irrespective of the treatment received. Oral administration of valganciclovir induced no noticeable side effect but reduced clinical signs of disease, infectious virus shedding and viraemia in ponies experimentally infected with the EHV-1 C2254 variant.
Host–Pathogen Interaction Involved in Trypanosoma cruzi Infection
Authors: Castillo Christian , Kemmerling Ulrike
Published: May 2022

Chagas disease, or American trypanosomiasis, caused by the protozoan parasite Trypanosoma cruzi (T. cruzi) [...]
Dynamics of Mycobacterium tuberculosis Lineages in Oman, 2009 to 2018
Authors: Al-Mahrouqi Sara , Ahmed Reham , Al-Azri Saleh , Al-Hamidhi Salama , Balkhair Abdullah A. , Al-Jardani Amina , Al-Fahdi Amira , Al-Balushi Laila , Al-Zadjali Samia , Adikaram Chamila , Al-Marhoubi Asmhan , Gadalla Amal , Babiker Hamza A.
Published: May 2022

Study aim. Effective Tuberculosis (TB) control measures in Oman have reduced the annual incidence of tuberculosis cases by 92% between 1981 and 2016. However, the current incidence remains above the program control target of <1 TB case per 100,000 population. This has been partly attributed to a high influx of migrants from countries with high TB burdens. The present study aimed to elucidate Mycobacterium tuberculosis infection dynamics among nationals and foreigners over a period of 10 years. Methods. The study examined TB cases reported between 2009 and 2018 and examined the spatial heterogeneity of TB cases and the distribution of M. tuberculosis genotypes defined by spoligotypes and MIRU-VNTR among Omanis and foreigners. Results. A total of 484 spoligoprofiles were detected among the examined isolates (n = 1295). These include 943 (72.8%) clustered and 352 (27.2%) unique isolates. Diverse M. tuberculosis lineages exist in all provinces in Oman, with most lineages shared between Omanis and foreigners. The most frequent spoligotypes were found to belong to EAI (318, 30.9%), CAS (310, 30.1%), T (154, 14.9%), and Beijing (88, 8.5%) lineages. However, the frequencies of these lineages differed between Omanis and foreigners. Of the clustered strains, 192 MTB isolates were further analysed via MIRU-VNTR. Each isolate exhibited a unique MIRU-VNTR profile, indicative of absence of ongoing transmission. Conclusions. TB incidence exhibits spatial heterogeneity across Oman, with high levels of diversity of M. tuberculosis lineages among Omanis and foreigners and sub-lineages shared between the two groups. However, MIRU-VNTR analysis ruled out ongoing transmission.
Biotechnological Evolution of siRNA Molecules: From Bench Tool to the Refined Drug
Authors: de Brito e Cunha Danielle , Frederico Ana , Azamor Tamiris , Melgaço Juliana , da Costa Neves Patricia , Bom Ana , Tilli Tatiana , Missailidis Sotiris
Published: May 2022

The depth and versatility of siRNA technologies enable their use in disease targets that are undruggable by small molecules or that seek to achieve a refined turn-off of the genes for any therapeutic area. Major extracellular barriers are enzymatic degradation of siRNAs by serum endonucleases and RNAases, renal clearance of the siRNA delivery system, the impermeability of biological membranes for siRNA, activation of the immune system, plasma protein sequestration, and capillary endothelium crossing. To overcome the intrinsic difficulties of the use of siRNA molecules, therapeutic applications require nanometric delivery carriers aiming to protect double-strands and deliver molecules to target cells. This review discusses the history of siRNAs, siRNA design, and delivery strategies, with a focus on progress made regarding siRNA molecules in clinical trials and how siRNA has become a valuable asset for biopharmaceutical companies.
Food-Derived β-Carboline Alkaloids Ameliorate Lipid Droplet Accumulation in Human Hepatocytes
Authors: Dibwe Dya Fita , Oba Saki , Takeishi Nire , Sakurai Toshihiro , Tsukui Takayuki , Chiba Hitoshi , Hui Shu-Ping
Published: May 2022

Lipid droplet accumulation (LDA) in hepatocytes is the initial stage of nonalcoholic fatty liver disease (NAFLD). In the search for natural compounds for the prevention of NAFLD, a series of β-carboline alkaloid derivatives, inspired by flazin and its derivative, newly identified in Crassostrea gigas Thunberg. extracts, were examined for LDA inhibition (LDAI) activity in oleic acid–loaded hepatocytes (HepG2). Eight compounds with a piperidine or pyridine C-ring were chemically synthesized (1–8). Among them, compounds 2 and 4 (flazin) with a carboxy group at C-3 and furfuryl alcohol moiety at C-1 showed low cytotoxicity and they exhibited significant LDAI activity. Compound 2 with piperidine C-ring was identified for the first time in C. gigas extract, and ameliorated the lipid accumulation with the LDAI value of 25.4%. Active compounds 2 and 4 significantly inhibited triacylglycerol species accumulation in cells. These compounds upregulated ATGL and downregulated SREBP1, FASN, and SCD1 genes, suggesting that they activated lipolysis and suppressed lipogenesis, respectively. These results suggest that β-carboline alkaloids, especially compounds 2 and 4, might be potentially useful for preventing NAFLD.
Formulation of Amphotericin B in PEGylated Liposomes for Improved Treatment of Cutaneous Leishmaniasis by Parenteral and Oral Routes
Authors: Ramos Guilherme S. , Vallejos Virgínia M. R. , Borges Gabriel S. M. , Almeida Raquel M. , Alves Izabela M. , Aguiar Marta M. G. , Fernandes Christian , Guimarães Pedro P. G. , Fujiwara Ricardo T. , Loiseau Philippe M. , Ferreira Lucas A. M. , Frézard Frédéric
Published: May 2022

Liposomal amphotericin B (AmB) or AmBisome® is the most effective and safe therapeutic agent for visceral leishmaniasis (VL), but its clinical efficacy is limited in cutaneous leishmaniasis (CL) and HIV/VL co-infection. The aim of this work was to develop a formulation of AmB in PEGylated liposomes and compare its efficacy to AmBisome® in a murine model of CL. Formulations of AmB in conventional and PEGylated liposomes were characterized for particle size and morphology, drug encapsulation efficiency and aggregation state. Those were compared to AmBisome® in Leishmania amazonensis-infected BALB/c mice for their effects on the lesion size growth and parasite load. The conventional and PEGylated formulations showed vesicles with 100–130 nm diameter and low polydispersity, incorporating more than 95% of AmB under the non-aggregated form. Following parenteral administration in the murine model of CL, the PEGylated formulation of AmB significantly reduced the lesion size growth and parasite load, in comparison to control groups, in contrast to conventional liposomal AmB. The PEGylated formulation of AmB was also effective when given by oral route on a 2-day regimen. This work reports for the first time that PEGylated liposomal AmB can improve the treatment of experimental cutaneous leishmaniasis by both parenteral and oral routes.
Rapid Identification of Easily-Confused Mineral Traditional Chinese Medicine (TCM) Based on Low-Wavenumber Raman and Terahertz Spectroscopy
Authors: Ge Meilan , Wang Yuye , Li Haibin , Wang Yu , Hu Changhao , Zhu Junfeng , Xu Degang , Wu Bin , Yao Jianquan
Published: May 2022

With the unique advantages of mineral TCMs gradually emerging in clinical treatment, health care, and precaution, it has played an important role in the international medical market. Commonly, mineral TCMs with similar appearance and different processing methods have different effects, but they are easy to be confused in preparation, storage, transportation, and other links, which affects the use and causes related problems. In this paper, six kinds of easily confused mineral TCMs, including coral skeleton, ophicalcitum, calamine, matrii sulfas exsiccatus, gypsum, and alumen, are rapidly characterized using Raman spectroscopy, which can be distinguished with different Raman peaks at 0–300 cm−1 due to the different lattice structure. The THz spectra of these mineral TCMs show that different mineral TCMs have different THz absorption coefficients at 0.3–2.0 THz. Furthermore, compared with the ineffectiveness of the Raman spectrum for differentiating mineral TCMs prepared with disparate processing methods, the terahertz absorption spectrum plays an active role in making up the limitation of low-wavenumber Raman spectroscopy. The combination of low-wavenumber Raman and THz spectroscopy provides a simple and feasible scheme for the identification of mineral TCMs, which could play an important role in the quality control of mineral TCMs.
AC/DC: The FERMI FEL Split and Delay Optical Device for Ultrafast X-ray Science
Authors: Simoncig Alberto , Manfredda Michele , Gaio Giulio , Mahne Nicola , Raimondi Lorenzo , Fava Claudio , Gerusina Simone , Gobessi Riccardo , Abrami Alessandro , Capotondi Flavio , De Angelis Dario , Menk Ralf Hendrik , Pancaldi Matteo , Pedersoli Emanuele , Zangrando Marco
Published: May 2022

Free-electron lasers (FELs) are the most advanced class of light-sources, by virtue of their unique capability to lase high-brightness pulses characterized by wavelengths spanning the extreme-ultraviolet, the soft and hard X-ray spectral domains, as well as by temporal lengths lying in the femtosecond (fs) timescale. The next step to push the current standards in ultrafast X-ray science is strongly linked to the possibility of engineering and exploiting time-resolved experiments exclusively for FELs pulses, ideally having different colors tunable at specific electronic resonance of the chemical elements. At the seeded FERMI FEL (Trieste, Italy) this goal is committed to the optical device known as AC/DC, which stands for the auto correlator/delay creator. AC/DC is designed to double the incoming FEL pulse splitting the photon beam by inserting a grazing incidence flat mirror, thus preserving the spectral and temporal properties, and further delaying one of these two pulses in time. It can independently tune the FEL pulses fluence on the two optical paths by means of solid-state filters, too. Here, we present a detailed description about this optical device. Strong emphasis is dedicated to the AC/DC opto-mechanical design and to the laser-based feedback systems implemented to compensate for any mismatch affecting the FEL optical trajectory, ascribable to both mechanical imperfections and paraxial errors rising during a temporal delay scan.