bluuebunny/crossref_metadata_2025 huggingface dataset:
Study of an Air Curtain in the Context of Individual Protection from Exposure to Coronavirus (SARS-CoV-2) Contained in Cough-Generated Fluid Particles
Authors: Sakharov Alexander S. , Zhukov Konstantin
Published: July 2020

The ongoing respiratory COVID-19 pandemic has heavily impacted the social and private lives of the majority of the global population. This infection is primarily transmitted via virus-laden fluid particles (i.e., droplets and aerosols) that are formed in the respiratory tract of infected individuals and expelled from the mouth in the course of breathing, talking, coughing, and sneezing. To mitigate the risk of virus transmission, in many places of the world, the public has been asked or even obliged to use face covers. It is plausible that in the years ahead we will see the use of face masks, face shields and respirators become a normal practice in our life. However, wearing face covers is uncomfortable in some situations, like, for example, in summer heat, while staying on beaches or at hotel swimming pools, doing exercises in gyms, etc. Also, most types of face cover become contaminated with time and need to be periodically replaced or disinfected. These nuisances are caused by the fact that face covers are based on material barriers, which prevent inward and outward propagation of aerosol and droplets containing the pathogen. Here, we study a non-material based protection barrier created by a flow of well directed down stream of air across the front of the open face. The protection is driven by dragging virus-laden particles inside the width of the air flow and hence, as a consequence, displacing them away from their primary trajectories. Applying well established gas-particle flow formalism, we analyzed the dynamics of aerosols and droplets at different regimes of the flow laying over the bodies of the fluid particles. The analysis allowed us to establish the rates of velocity gain of the fluid particles of dimensions relevant for the pathogen transmissions, while they are crossing the width of the air barrier. On the basis of this analysis, we provide a comprehensive study of the protection effectiveness of the air barrier for a susceptible individual located indoor, in an infected environment. Our study shows that such, potentially portable, air curtains can effectively provide both inward and outward protection and serve as an effective personal protective equipment (PPE) mitigating human to human transmission of virus infection like COVID-19.
Effects of Soil Fertilization on Terpenoids and Other Carbon-Based Secondary Metabolites in Rosmarinus officinalis Plants: A Comparative Study
Authors: Bustamante Maria Ángeles , Michelozzi Marco , Barra Caracciolo Anna , Grenni Paola , Verbokkem Janine , Geerdink Peter , Safi Carl , Nogues Isabel
Published: July 2020

Rosmarinus officinalis is an evergreen aromatic plant with important commercial interest as it contains numerous essential oils (composed of terpenoid compounds) and phenolic constituents (natural antioxidant compounds). This work aims at evaluating the concomitant effects of different inorganic and organic fertilization treatments and the subsequent increases in soil nutrient availability on terpenoids and other carbon-based secondary metabolites, e.g., flavonoids and phenolic compounds, in Rosmarinus officinalis leaves. The results showed that, as expected, the structural carbohydrate content (lignocellulosic compounds) in stems was higher in fertilized plants than in controls. Additionally, positive correlations were observed of the absolute amounts of total terpenoids and some single terpenoid compounds with N or P contents in leaves. On the contrary, the phenolic and flavonoid concentrations in all the rosemary plant parts were lower with the fertilization treatments. Indeed, negative correlations between the phenolic compounds (and flavonoids) and N in rosemary leaves were also found. Overall, the results suggest that the terpenoid production’s response to fertilization was due to N, which is essential for protein synthesis and terpene synthase activity, and to P, which is necessary for the synthesis of both terpenoid precursors and ATP and NADPH, also needed for terpenoid synthesis. On the other hand, the basis for the fertilization’s effects on the production of phenolic compounds is the direct nitrogen trade-off between growth and the shikimic acid pathway by which phenolics compounds are synthesized.
Review of the Impact of Apple Fruit Ripening, Texture and Chemical Contents on Genetically Determined Susceptibility to Storage Rots
Authors: Nybom Hilde , Ahmadi-Afzadi Masoud , Rumpunen Kimmo , Tahir Ibrahim
Published: July 2020

Fungal storage rots like blue mould, grey mould, bull’s eye rot, bitter rot and brown rot destroy large amounts of the harvested apple crop around the world. Application of fungicides is nowadays severely restricted in many countries and production systems, and these problems are therefore likely to increase. Considerable variation among apple cultivars in resistance/susceptibility has been reported, suggesting that efficient defence mechanisms can be selected for and used in plant breeding. These are, however, likely to vary between pathogens, since some fungi are mainly wound-mediated while others attack through lenticels or by infecting blossoms. Since mature fruits are considerably more susceptible than immature fruits, mechanisms involving fruit-ripening processes are likely to play an important role. Significant associations have been detected between the susceptibility to rots in harvested fruit and various fruit maturation-related traits like ripening time, fruit firmness at harvest and rate of fruit softening during storage, as well as fruit biochemical contents like acidity, sugars and polyphenols. Some sources of resistance to blue mould have been described, but more research is needed on the development of spore inoculation methods that produce reproducible data and can be used for large screenings, especially for lenticel-infecting fungi.
Evaluation of Wild Potato Germplasm for Tuber Starch Content and Nitrogen Utilization Efficiency
Authors: Bachmann-Pfabe Silvia , Dehmer Klaus J.
Published: July 2020

Potato wild relatives provide a considerable source of variation for important traits in cultivated potato (Solanum tuberosum L.) breeding. This study evaluates the variation of tuber starch content and nitrogen utilization efficiency (NutE) in wild potato germplasm. For the experiments regarding starch content, 28 accessions of ten different tuber-bearing wild Solanum-species were chosen, and in vitro plantlets were raised from seeds. Twenty plantlets (= genotypes) per accession were then cultivated in the greenhouse until natural senescence and tuber starch content was determined. The average tuber starch content across all genotypes tested was 21.7% of fresh mass. Contents above 28% of fresh mass were found in 50 genotypes, belonging to the species S. chacoense, S. commersonii, S. jamesii, and S. pinnatisectum. Subsequently, 22 wild genotypes revealing high tuber starch contents and four modern varieties of cultivated potato were studied as in vitro plantlets under optimal and low N supply (30 and 7.5 mmol L−1 N). Low N supply lead to a genotype-dependent reduction of shoot dry mass between 13 and 46%. The majority of the wild types also reduced root dry mass by 26 to 62%, while others maintained root growth and even exceeded the NutE of the varieties under low N supply. Thus, wild potato germplasm appears superior to cultivars in terms of tuber starch contents and N utilization efficiency, which should be investigated in further studies.
Arabidopsis Plastid-RNA Polymerase RPOTp Is Involved in Abiotic Stress Tolerance
Authors: Lidón-Soto Abel , Núñez-Delegido Eva , Pastor-Martínez Iván , Robles Pedro , Quesada Víctor
Published: July 2020

Plastid gene expression (PGE) must adequately respond to changes in both development and environmental cues. The transcriptional machinery of plastids in land plants is far more complex than that of prokaryotes. Two types of DNA-dependent RNA polymerases transcribe the plastid genome: a multimeric plastid-encoded polymerase (PEP), and a monomeric nuclear-encoded polymerase (NEP). A single NEP in monocots (RPOTp, RNA polymerase of the T3/T7 phage-type) and two NEPs in dicots (plastid-targeted RPOTp, and plastid- and mitochondrial-targeted RPOTmp) have been hitherto identified. To unravel the role of PGE in plant responses to abiotic stress, we investigated if Arabidopsis RPOTp could function in plant salt tolerance. To this end, we studied the sensitivity of T-DNA mutants scabra3-2 (sca3-2) and sca3-3, defective in the RPOTp gene, to salinity, osmotic stress and the phytohormone abscisic acid (ABA) required for plants to adapt to abiotic stress. sca3 mutants were hypersensitive to NaCl, mannitol and ABA during germination and seedling establishment. Later in development, sca3 plants displayed reduced sensitivity to salt stress. A gene ontology (GO) analysis of the nuclear genes differentially expressed in the sca3-2 mutant (301) revealed that many significantly enriched GO terms were related to chloroplast function, and also to the response to several abiotic stresses. By quantitative RT-PCR (qRT-PCR), we found that genes LHCB1 (LIGHT-HARVESTING CHLOROPHYLL a/b-BINDING1) and AOX1A (ALTERNATIVE OXIDASE 1A) were respectively down- and up-regulated in the Columbia-0 (Col-0) salt-stressed plants, which suggests the activation of plastid and mitochondria-to-nucleus retrograde signaling. The transcript levels of genes RPOTp, RPOTmp and RPOTm significantly increased in these salt-stressed seedlings, but this enhanced expression did not lead to the up-regulation of the plastid genes solely transcribed by NEP. Similar to salinity, carotenoid inhibitor norflurazon (NF) also enhanced the RPOTp transcript levels in Col-0 seedlings. This shows that besides salinity, inhibition of chloroplast biogenesis also induces RPOTp expression. Unlike salt and NF, the NEP genes were significantly down-regulated in the Col-0 seedlings grown in ABA-supplemented media. Together, our findings demonstrate that RPOTp functions in abiotic stress tolerance, and RPOTp is likely regulated positively by plastid-to-nucleus retrograde signaling, which is triggered when chloroplast functionality is perturbed by environmental stresses, e.g., salinity or NF. This suggests the existence of a compensatory mechanism, elicited by impaired chloroplast function. To our knowledge, this is the first study to suggest the role of a nuclear-encoded plastid-RNA polymerase in salt stress tolerance in plants.
QTL Mapping and Prediction of Haploid Male Fertility Traits in Maize (Zea mays L.)
Authors: Jiao Yanyan , Li Jinlong , Li Wei , Chen Ming , Li Mengran , Liu Wenxin , Liu Chenxu , Chen Shaojiang
Published: July 2020

Chromosome doubling of maize haploids is a bottleneck in the large-scale application of doubled haploid (DH) technology. Spontaneous chromosome doubling (SCD) of haploid has been taken as an important method in the production of DH lines and low haploid male fertility (HMF) is a main limiting factor for the use of SCD. To study its genetic basis, haploids of 119 DH lines derived from a cross between inbred lines Qi319 and Chang7-2 were used to map the quantitative trait locus (QTL) contributing to HMF. Three traits including anther emergence rate (AER), anther emergence score (AES) and pollen production score (PPS) of the haploid population were evaluated at two locations. The heritability of the three traits ranged from 0.70 to 0.81. The QTL contributing to AER, AES and PPS were identified on the chromosomes 1, 2, 3, 4, 5, 7, 9 and 10. Five major QTL, qAER5-1, qAER5-2, qAES3, qPPS1 and qPPS5, were found and each could explain more than 15% of the phenotypic variance at least in one environment. Two major QTL, qPPS1 and qPPS5, and two minor QTL, qAES2 and qAER3, were repeatedly detected at both locations. To increase the application efficiency of HMF in breeding programs, genomic prediction for the three traits were carried out with ridge regression best linear unbiased prediction (rrBLUP) and rrBLUP adding QTL effects (rrBLUP-QTL). The prediction accuracies of rrBLUP-QTL were significantly higher than that by rrBLUP for three traits (p < 0.001), which indirectly indicates these QTL were effective. The prediction accuracies for PPS were 0.604 (rrBLUP) and 0.703 (rrBLUP-QTL) across both locations, which were higher than that of AER and AES. Overall, this study provides important information to understand the genetic architecture of SCD of maize haploids.
Exogenous Carbon Compounds Modulate Tomato Root Development
Authors: González-Hernández Ana Isabel , Scalschi Loredana , García-Agustín Pilar , Camañes Gemma
Published: July 2020

NO3− is not only a nutrient, but also a signaling compound that plays an important role in several plant processes, like root development. The present study aimed to investigate the effect of three different exogenous C compounds (sucrose, glucose, 2-oxoglutarate) added to NO3− nutrition on C/N, auxin and antioxidant metabolisms in 10-day-old tomato seedlings. Sucrose and glucose supplementation enhanced primary root (PR) length, lateral root number and root density, while 2-oxoglutarate negatively affected them. This phenomenon was accompanied by a slight increase in NRT2.1 and GS1 gene expression, together with an increase in LAX2 and LAX3 and a decrease in LAX4 in the roots growing under sucrose and glucose sources. The addition of 2-oxoglutarate enhanced the expression of NiR, GDH, PEPC1, LAX1, LAX3 and the antioxidant gene SOD Cl. Taken together, these findings contribute to a better understanding of how these C sources can modulate N uptake and C/N, auxin and antioxidant gene expression, which could be useful for improving nitrogen use efficiency.
Nematicidal Efficacy of Milbemectin against Root-Knot Nematodes
Authors: Talavera-Rubia Miguel , Vela-Delgado Maria Dolores , Verdejo-Lucas Soledad
Published: July 2020

The nematicidal efficacy of milbemectin and its commercial formulate Milbeknock® on (i) egg hatching, (ii) juvenile motility and (iii) infective capacity of root-knot nematodes was evaluated in vitro and in planta assays. Serial dilutions of pure milbemectin were tested against nematode eggs and juveniles and lethal concentrations LC50 and LC90 calculated. Exposure of egg masses to milbemectin at a concentration of 30 μg/mL for 72 h reduced egg hatching by 52%. The increase in exposure time to 240 h did not increase the egg hatching inhibition at the highest concentration 30 μg/mL (53%) but reduced egg hatching at 15 and 7 μg/mL by 35 and 24%, respectively, when compared to untreated controls. The inhibitory effect of milbemectin on juvenile motility ranged from 41 to 87% depending on its concentration, and this effect was persistent after rinsing the juveniles in water. The probabilistic dose–response model indicated that lethal concentrations of milbemectin for juvenile motility were LC50: 7.4 μg/mL and LC90: 29.9 μg/mL. The pre-plant application of Milbeknock® to soils infested with the nematode reduced its infective capacity by 98–99% compared to untreated soils in pot experiments. Milbeknock® reduced nematode soil population densities by 50–60% in natural infestations under field conditions. Milbemectin shows a high level of efficacy against root-knot nematodes as it reduces egg hatching, persistently immobilizes nematode juveniles, and reduces tomato root infection.
Knock-Down the Expression of Brassinosteroid Receptor TaBRI1 Reduces Photosynthesis, Tolerance to High Light and High Temperature Stresses and Grain Yield in Wheat
Authors: Fang Jingjing , Zhu Weiqi , Tong Yiping
Published: July 2020

Brassinosteroid (BR)-deficient or -insensitive mutants exhibited altered plant architecture with the potential to impact yield, the underlying physiological and molecular mechanisms are still to be explored. In this study, we cloned three BR receptor homologous genes TaBRI1-A1, -B1 and -D1 from hexaploid wheat (Triticum estivum L.) and further isolated the TaBRI1-A1, TaBRI1-D1 deletion mutants from the ion beam-induced mutants of variety Xiaoyan81, TaBRI1-A1 and TaBRI1-D1 in which the expression of total receptor TaBRI1 was significantly decreased. The TaBRI1 knock-down mutants exhibited relatively erect leaves and a significant decrease in the 1000-grain weight. Further studies showed that TaBRI1 knock-down mutants showed a significant reduction in photosynthetic rate during the whole grain-filling stage. TaBRI1 knock-down plants generated by TaBRI1-A1, TaBRI1-D1 deletion or using virus-induced gene silencing exhibited the reduction in the efficiency of photosystem II (PSII) (Fv/Fm, ΦPSII and electron transport rate, ETR) especially under high light and high temperature stresses. The 24-epibrassinolide (EBR) treatment increased CO2 assimilation rate in the wild type under both normal and high light and high temperature stresses conditions, but this increasing effect was not observed in the TaBRI1 knock-down mutants. Meanwhile, the expression levels of BR biosynthetic genes including TaDWARF4, TaCPD1 and TaCPD90C1 is not decreased or decreased to a lesser extent in the TaBRI1 knock-down mutants after EBR treatment. These results suggested that TaBRI1 is required for maintaining photosynthesis and tolerance to high light and high temperature stresses both of which are important for grain yield and will be a possible engineered target to control plant photosynthesis and yields in wheat.
Quantifying Metal Contamination and Potential Uptake by Phragmites australis Adans. (Poaceae) Along a Subtropical River System
Authors: Netshiongolwe Ndivhuwo R. , Cuthbert Ross N. , Maenetje Mokgale M. , Chari Lenin D. , Motitsoe Samuel N. , Wasserman Ryan J. , Munyai Linton F. , Dalu Tatenda
Published: July 2020

Metal pollution is pervasive across terrestrial and aquatic ecosystems owing to anthropogenic activities. Sediments can accrue high concentrations of metals and act as secondary sources, and thus may be valuable indicators of metal contamination across spatiotemporal scales. In aquatic systems, the extent of metal pollution may be further mediated by transference among sediments and living organisms, with plant metal contaminants potentially predictive of underlying sediment concentrations. The present study thus quantifies the extent of metal pollutants (Na, K, Ca, Mg, Cu, Zn, Mn, B, Fe) across multiple study sites and seasons (cool-dry, hot-wet, hot-dry) in a subtropical river system. Furthermore, uptake by a key macrophyte species, Phragmites australis, was examined and correlated with sediment pollution levels among different plant parts. Overall, sediment pollution load indices differed seasonally, being significantly highest during the cool-dry season irrespective of sampling location, suggesting that periods with reduced water flows can exacerbate metal pollution levels in riverine sediments. Also, metal concentrations were highest in upstream wetland sites, indicating a capacity for metal sink effects in these areas. Overall, macrophytes contained high concentrations of select metals, however composition and concentrations differed across plant parts, with roots containing particularly high concentrations of Fe and B. Correlations between sediment and macrophyte concentrations were mostly non-significant, whilst stem Mn and Fe concentrations correlated significantly negatively and positively to sediment concentrations, respectively. The present study identifies key spatiotemporal differences in multiple metal contaminants in an understudied subtropical aquatic system that align with hydrological regime differences. Whilst macrophytes were not found to be major accumulators, or predictors, of metal contaminants in this study, they may collectively play a central role in concentration regulation in aquatic systems.
Overview of Schistochilopsis (Hepaticae) in Pacific Asia with the Description Protochilopsis gen. nov.
Authors: Bakalin Vadim A. , Fedosov Vladimir E. , Maltseva Yulia D. , Milyutina Irina A. , Klimova Ksenia G. , Nguyen Hung Manh , Troitsky Aleksey V.
Published: July 2020

The taxonomic diversity center of Schistochilopsis is in East Asia, where the genus also shows the highest genetic diversity and morphological plasticity. The integrative survey of Schistochilopsis in East Asia was the main goal of the present account. Plant materials were obtained from recent collections made by authors in various parts of amphi-Pacific Asia; several types of specimens were also studied. The study includes phylogenetic reconstructions from nuclear ITS1,2, chloroplast trnL and trnG sequences, and anatomo-morphological, biogeographical, and taxonomical analyses. As a result, it was concluded that S. obscura should be transferred to Lophozia s. str. and S. grandiretis to the newly described genus Protochilopsis. Lophozia boliviensis was found to be part of the Andean–Sino-Himalayan taxon belonging to Schistochilopsis. The species status of S. hyperarctica and S. opacifolia was not confirmed. Substantial genetic variation is observed within S. incisa with possible cryptic sympatric distributed entities. The taxonomical section of the paper provides a discussion on the status, distinctive morphological traits, distribution, and ecology supplemented with the morphological description for poorly understood taxa. In the vast majority of cases, the illustrations and photographs made from the types and living material are provided.
Identification of the Volatile Compounds and Observation of the Glandular Trichomes in Opisthopappus taihangensis and Four Species of Chrysanthemum
Authors: Guo Yanhong , Zhang Tengxun , Zhong Jian , Ba Tingting , Xu Ting , Zhang Qixiang , Sun Ming
Published: July 2020

Opisthopappus taihangensis (Ling) Shih, a wild relative germplasm of chrysanthemum, releases a completely different fragrance from chrysanthemum species. We aimed to identify the volatile compounds of the leaves of O. taihangensis and four other Chrysanthemum species using headspace solid-phase micro-extraction combined with gas chromatography-mass spectrometry (HS-SPME-GC/MS). In total, 70 compounds were detected, and terpenoids accounted for the largest percentage in these five species. Many specific compounds were only emitted from O. taihangensis and not from the other four species. In particular, 1,8-cineole could be responsible for the special leaf fragrance of O. taihangensis as it accounted for the largest proportion of the compounds in O. taihangensis but a small or no proportion at all in other species. The glandular trichomes (GTs) in the leaves are the main organs responsible for the emission of volatiles. To explore the relationship between the emissions and the density of the GTs on the leaf epidermis, the shape and density of the GTs were observed and calculated, respectively. The results showed that the trichomes have two shapes in these leaves: T-shaped non-glandular trichomes and capitate trichomes. Histochemical staining analyses indicated that terpenoids are mainly emitted from capitate glandular trichomes. Correlation analysis showed that the volatile amount of terpenoids is highly related to the density of capitate trichomes. In O. taihangensis, the terpenoids content and density of capitate trichomes are the highest. We identified the diversity of leaf volatiles from O. taihangensis and four other Chrysanthemum species and found a possible relationship between the content of volatile compounds and the density of capitate trichomes, which explained the cause of the fragrance of O. taihangensis leaves.
Bio-Based Polyamide 1010 with a Halogen-Free Flame Retardant Based on Melamine–Gallic Acid Complex
Authors: Levinta Nicoleta , Corobea Mihai Cosmin , Vuluga Zina , Nicolae Cristian-Andi , Gabor Augusta Raluca , Raditoiu Valentin , Osiac Mariana , Teodorescu George-Mihail , Teodorescu Mircea
Published: July 2020

This work aims at developing polyamide 1010 (PA1010) composites with improved fire behavior using a halogen-free flame-retardant system based on melamine (Me) and gallic acid (GA) complexes (MA). The MA complexes were formed by hydrogen bonding, starting from 1:2, 1:1, 2:1 Me:GA molar ratios. PA1010 composites were obtained by melt mixing, followed by compression molding. MA provided a plasticizing effect on the PA1010 matrix by decreasing the glass transition temperature. The influence of MA on PA1010 chain packaging was highlighted in the X-ray diffraction patterns, mainly in the amorphous phase, but affected also the α and γ planes. This was reflected in the dynamic mechanical properties by the reduction of the storage modulus. H-bonds occurrence in MA complexes, improved the efficiency in the gaseous form during fire exposure, facilitating the gas formation and finally reflected in thermal stability, thermo-oxidative stability, LOI results, and vertical burning behavior results. PA1010 containing a higher amount of GA in the complex (MA12) displayed a limiting oxygen index (LOI) value of 33.6%, much higher when compared to neat PA1010 (25.8%). Vertical burning tests showed that all the composites can achieve the V-0 rating in contrast with neat PA1010 that has V-2 classification.
The Photoinitiator Lithium Phenyl (2,4,6-Trimethylbenzoyl) Phosphinate with Exposure to 405 nm Light Is Cytotoxic to Mammalian Cells but Not Mutagenic in Bacterial Reverse Mutation Assays
Authors: Nguyen Alexander K. , Goering Peter L. , Elespuru Rosalie K. , Sarkar Das Srilekha , Narayan Roger J.
Published: July 2020

Lithium phenyl (2,4,6-trimethylbenzoyl) phosphinate (LAP) is a free radical photo-initiator used to initiate free radical chain polymerization upon light exposure, and is combined with gelatin methacryloyl (GelMA) to produce a photopolymer used in bioprinting. The free radicals produced under bioprinting conditions are potentially cytotoxic and mutagenic. Since these photo-generated free radicals are highly-reactive but short-lived, toxicity assessments should be conducted with light exposure. In this study, photorheology determined that 10 min exposure to 9.6 mW/cm2 405 nm light from an LED light source fully crosslinked 10 wt % GelMA with >3.4 mmol/L LAP, conditions that were used for subsequent cytotoxicity and mutagenicity assessments. These conditions were cytotoxic to M-1 mouse kidney collecting duct cells, a cell type susceptible to lithium toxicity. Exposure to ≤17 mmol/L (0.5 wt %) LAP without light was not cytotoxic; however, concurrent exposure to ≥3.4 mmol/L LAP and light was cytotoxic. No condition of LAP and/or light exposure evaluated was mutagenic in bacterial reverse mutation assays using S. typhimurium strains TA98, TA100 and E. coli WP2 uvrA. These data indicate that the combination of LAP and free radicals generated from photo-excited LAP is cytotoxic, but mutagenicity was not observed in bacteria under typical bioprinting conditions.
Present Status in Polymeric Mouthguards. A Future Area for Additive Manufacturing?
Authors: Sousa Ana M. , Pinho Ana C. , Messias Ana , Piedade Ana P.
Published: July 2020

Athletes from contact sports are more prone to orofacial injuries because of the exposure to possible shocks and collisions derived from physical proximity. The use of protector polymeric mouthguards proved to be useful in the prevention of the described injuries. There are different types of mouthguards with varying ranges of protection and prices, but they are all made from polymers and share the same propose: to absorb and dissipate the impact energy resulting from the shocks. As they are used inside the mouth, they should not impair breathing and speaking nor compromise the comfort of the athlete. However, the ideal mouthguard is yet to be created. The choice of the most appropriate polymeric material and the standard required properties have not yet been reported. Regardless of the numerous studies in this field, normalized control parameters for both material characterization and mouthguard fabrication are absent. This paper aims to present a review of the current types of available mouthguards and their properties/characteristics. Moreover, a detailed description of the most common polymers for the fabrication of mouthguards, together with the manufacturing techniques, are discussed.
Comparative Study of Aromatic and Cycloaliphatic Isocyanate Effects on Physico-Chemical Properties of Bio-Based Polyurethane Acrylate Coatings
Authors: Mudri Nurul Huda , Abdullah Luqman Chuah , Aung Min Min , Salleh Mek Zah , Awang Biak Dayang Radiah , Rayung Marwah
Published: July 2020

Crude jatropha oil (JO) was modified to form jatropha oil-based polyol (JOL) via two steps in a chemical reaction known as epoxidation and hydroxylation. JOL was then reacted with isocyanates to produce JO-based polyurethane resin. In this study, two types of isocyanates, 2,4-toluene diisocyanate (2,4-TDI) and isophorone diisocyanate (IPDI) were introduced to produce JPUA-TDI and JPUA-IPDI respectively. 2,4-TDI is categorised as an aromatic isocyanate whilst IPDI is known as a cycloaliphatic isocyanate. Both JPUA-TDI and JPUA-IPDI were then end-capped by the acrylate functional group of 2-hydroxyethyl methacrylate (HEMA). The effects of that isocyanate structure were investigated for their physico, chemical and thermal properties. The changes of the functional groups during each synthesis step were monitored by FTIR analysis. The appearance of urethane peaks was observed at 1532 cm−1, 1718 cm−1 and 3369 cm−1 while acrylate peaks were detected at 815 cm−1 and 1663 cm−1 indicating that JPUA was successfully synthesised. It was found that the molar mass of JPUA-TDI was doubled compared to JPUA-IPDI. Each resin showed a similar degradation pattern analysed by thermal gravimetric analysis (TGA). For the mechanical properties, the JPUA-IPDI-based coating formulation exhibited a higher hardness value but poor adhesion compared to the JPUA-TDI-based coating formulation. Both types of jatropha-based polyurethane acrylate may potentially be used in an ultraviolet (UV) curing system specifically for clear coat surface applications to replace dependency on petroleum-based chemicals.
Finite Element Analysis on Bingham–Papanastasiou Viscoplastic Flow in a Channel with Circular/Square Obstacles: A Comparative Benchmarking
Authors: Mehmood Asif , Khan Waqar A. , Mahmood Rashid , Rehman Khalil Ur
Published: July 2020

A CFD (computational fluid dynamics) analysis was carried out for the Bingham viscoplastic fluid flow simulations around cylinders of circular and square shapes. The governing equations in space were discretized with the finite element approach via a weak formulation and utilizing Ladyzhenskaya–Babuška–Brezzi-stable pair Q 2 / P 1 disc for approximation of the velocity and pressure profiles. The discrete non-linear system was linearized through Newton’s method, and a direct linear solver was iterated as an inner core solver. The study predicts the functional dependence and impact of Bingham number, B n , on the drag coefficient and lift coefficient. The effect of the shape of an obstacle is also provided by providing comparative data for the hydrodynamic forces with the published results.
Compartment Model of Mixing in a Bubble Trap and Its Impact on Chromatographic Separations
Authors: Beck Jürgen , Heymann William , von Lieres Eric , Hahn Rainer
Published: July 2020

Chromatography equipment includes hold-up volumes that are external to the packed bed and usually not considered in the development of chromatography models. These volumes can substantially contribute to band-broadening in the system and deteriorate the predicted performance. We selected a bubble trap of a pilot scale chromatography system as an example for a hold-up volume with a non-standard mixing behavior. In a worst-case scenario, the bubble trap is not properly flushed before elution, thus causing the significant band-broadening of the elution peak. We showed that the mixing of buffers with different densities in the bubble trap device can be accurately modeled using a simple compartment model. The model was calibrated at a wide range of flow rates and salt concentrations. The simulations were performed using the open-source software CADET, and all scripts and data are published with this manuscript. The results illustrate the importance of including external holdup volumes in chromatography modeling. The band-broadening effect of tubing, pumps, valves, detectors, frits, or any other zones with non-standard mixing behavior can be considered in very similar ways.
Potential of Jatropha curcas L. as Biodiesel Feedstock in Malaysia: A Concise Review
Authors: Che Hamzah Nurul Husna , Khairuddin Nozieana , Siddique Bazlul Mobin , Hassan Mohd Ali
Published: July 2020

Fluctuation in fossil fuel prices and the increasing awareness of environmental degradation have prompted the search for alternatives from renewable energy sources. Biodiesel is the most efficient alternative to fossil fuel substitution because it can be properly modified for current diesel engines. It is a vegetable oil-based fuel with similar properties to petroleum diesel. Generally, biodiesel is a non-toxic, biodegradable, and highly efficient alternative for fossil fuel substitution. In Malaysia, oil palm is considered as the most valuable commodity crop and gives a high economic return to the country. However, the ethical challenge of food or fuel makes palm oil not an ideal feedstock for biodiesel production. Therefore, attention is shifted to non-edible feedstock like Jatropha curcas Linnaeus (Jatropha curcas L.). It is an inedible oil-bearing crop that can be processed into biodiesel. It has a high-seed yield that could be continually produced for up to 50 years. Furthermore, its utilization will have zero impact on food sources since the oil is poisonous for human and animal consumption. However, Jatropha biodiesel is still in its preliminary phase compared to palm oil-based biodiesel in Malaysia due to a lack of research and development. Therefore, this paper emphasizes the potential of Jatropha curcas as an eco-friendly biodiesel feedstock to promote socio-economic development and meet significantly growing energy demands even though the challenges for its implementation as a national biodiesel program might be longer.
A Systematic Review of the Effectiveness of Cell-Based Therapy in Repairing Peripheral Nerve Gap Defects
Authors: Sahar Muhammad , Barton Matthew , Tansley Geoffrey
Published: July 2020

Nerve prostheses are widely utilized to reconstruct segmental (gap) defects in peripheral nerves as an alternative to nerve grafting. However, with increasing gap length, the effectiveness of a nerve prosthesis becomes sub-optimal, which subsequently has made repairing larger gaps in peripheral nerves a significant challenge in the field of regenerative medicine. Recently, the structure of nerve prostheses has been significantly revised, which interestingly, has provided a promising avenue for the housing and proliferation of supportive cells. In this systematic review, cell implantation in synthetic nerve prostheses to enhance the regenerative capability of an injured nerve with a focus on identifying the cell type and mode of cell delivery is discussed. Of interest are the studies employing supportive cells to bridge gaps greater than 10 mm without the aid of nerve growth factors. The results have shown that cell therapy in conjunction with nerve prostheses becomes inevitable and has dramatically boosted the ability of these prostheses to maintain sustainable nerve regeneration across larger gaps and helped to attain functional recovery, which is the ultimate goal. The statistical analysis supports the use of differentiated bone-marrow-derived mesenchymal stem cells suspended in oxygen-carrying hydrogels in chitosan prostheses for bridging gaps of up to 40 mm; however, based on the imperfect repair outcomes, nerve grafting should not yet be replaced altogether.
Chinese Temple Networks in Southeast Asia: A WebGIS Digital Humanities Platform for the Collaborative Study of the Chinese Diaspora in Southeast Asia
Authors: Yan Yingwei , Dean Kenneth , Feng Chen-Chieh , Hue Guan Thye , Koh Khee-heong , Kong Lily , Ong Chang Woei , Tay Arthur , Wang Yi-chen , Xue Yiran
Published: July 2020

This article introduces a digital platform for collaborative research on the Chinese diaspora in Southeast Asia, focusing on networks of Chinese temples and associations extending from Southeast China to the various port cities of Southeast Asia. The Singapore Historical Geographic Information System (SHGIS) and the Singapore Biographical Database (SBDB) are expandable WebGIS platforms gathering and linking data on cultural and religious networks across Southeast Asia. This inter-connected platform can be expanded to cover not only Singapore but all of Southeast Asia. We have added layers of data that go beyond Chinese Taoist, Buddhist, and popular god temples to also display the distributions of a wide range of other religious networks, including Christian churches, Islamic mosques, Hindu temples, and Theravadin, which are the Taiwanese, Japanese and Tibetan Buddhist monasteries found across the region. This digital platform covers a larger area than the Taiwan History and Culture in Time and Space (THCTS) historical GIS platform but is more regionally focused than the ECAI (Electronic Cultural Atlas Initiative) By incorporating Chinese inscriptions, extensive surveys of Chinese temples and associations, as well as archival and historical sources, this platform provides new materials and new perspectives on the Chinese diaspora in Southeast Asia. This paper: (1) outlines key research questions underlying these digital humanities platforms; (2) describes the overall architecture and the kinds of data included in the SHGIS and the SBDB; (3) reviews past research on historical GIS; and provides (4) a discussion of how incorporating Chinese epigraphy of Southeast Asia into these websites can help scholars trace networks across the entire region, potentially enabling comparative work on a wide range of religious networks in the region. Part 5 of the paper outlines technical aspects of the WebGIS platform.
Indigenous Labor and Land Resources: Guarani–Kaiowa’s Politico–Economic and Ethnic Challenges
Authors: Ioris Antonio A. R.
Published: July 2020

The article deals with the meaning and the management of land-based resources by indigenous peoples, which are analyzed through an assessment of the lived spaces of the Guarani–Kaiowa indigenous people in the Brazilian state of Mato Grosso do Sul. The discussion follows an analytical framework that is focused on land, labor and ethnicity. These interconnected politico-economic categories provide the basis for understanding the violence and exploitation perpetrated against indigenous groups, as well as their capacity to reclaim ancestral territories lost to extractivism and agribusiness development. Empirical results indicate that ethnicity is integral to labor and land management processes. In the case of the Guarani–Kaiowa, not only have they become refugees in their own lands due to racist discrimination, but also their labor has been incorporated in the regional economy through interrelated peasantification and proleterianization tendencies. The result is a complex situation that combines major socio-spatial asymmetries with the strategic, exploitative use of land and labor and the growing political contestation by the indigenous groups.
Using Satellite Gravity and Hydrological Data to Estimate Changes in Evapotranspiration Induced by Water Storage Fluctuations in the Three Gorges Reservoir of China
Authors: Zheng Yuhao , Wang Linsong , Chen Chao , Fu Zhengyan , Peng Zhenran
Published: July 2020

The change in water storage driven by the Three Gorges Project directly affects the terrestrial water migration and redistribution in the Yangtze River Basin (YRB). As a result, a new water balance is established and regional evapotranspiration (ET) fluctuates in the process. In this paper, data from multiple-sources including from the Gravity Recovery and Climate Experiment (GRACE) satellite, land surface models (LSMs), remote sensing, and in-situ observations were used to monitor the temporal and spatial evolution of terrestrial water and estimate changes in ET in the Three Gorges Reservoir (TGR) from 2002 to 2016. Our results showed that GRACE data scaled using the scale factor method significantly improved the signal amplitude and highlighted its spatial differences in the TGR area. Combining GRACE with surface hydrological observations, ET in the TGR area was estimated to have overall change characteristics highly consistent with results from the MOD16 Moderate Resolution Imaging Spectroradiometer (MODIS), and the uncertainties of monthly ET are mainly from TWS changes derived by GRACE uncertainties such as measurement errors and leakage errors. During our study period, the cyclical ET was mainly driven by climate precipitation but short-term (monthly) ET in the TGR area was also directly affected by human-driven water storage. For example, rising water levels in the three water storage stages (2003, 2006, and 2008) caused an abnormal increase in regional ET (up to 22.4 cm/month, 19.2 cm/month and 29.5 cm/month, respectively). Usually, high precipitation will cause increase in ET but the high precipitation during the water release periods (spring and summer) did not have a significant impact on the increased ET due to the water level in the TGR having decreased 30 m in this stage. Our results also indicate that the short-term fluctuations in flooded area and storage capacity of the TGR, i.e., the man-made mass changes in the main branch and tributaries of the Yangtze River, were the main factors that influenced the ET. This further illustrated that a quantitative estimation of changes in the ET in the TGR allows for a deeper understanding of the water balance in the regional land water cycle process as driven by both climate and human factors.
Hydrometeor Distribution and Linear Depolarization Ratio in Thunderstorms
Authors: Sokol Zbyněk , Minářová Jana , Fišer Ondřej
Published: July 2020

The distribution of hydrometeors in thunderstorms is still under investigation as well as the process of electrification in thunderclouds leading to lightning discharges. One indicator of cloud electrification might be high values of the Linear Depolarization Ratio (LDR) at higher vertical levels. This study focuses on LDR values derived from vertically pointing cloud radars and the distribution of five hydrometeor species during 38 days with thunderstorms which occurred in 2018 and 2019 in Central Europe, close to our radar site. The study shows improved algorithms for de-aliasing, the derivation of vertical air velocity and the classification of hydrometeors in clouds using radar data. The comparison of vertical profiles with observed lightning discharges in the vicinity of the radar site (≤1 km) suggested that cloud radar data can indirectly identify “lightning” areas by high LDR values observed at higher gates due to the alignment of ice crystals, likely because of an intensified electric field in thunderclouds. Simultaneously, the results indicated that at higher gates, there is a mixture of several hydrometeor species, which suggests a well-known electrification process by collisions of hydrometeors.
High-Resolution Coherency Functionals for Improving the Velocity Analysis of Ground-Penetrating Radar Data
Authors: Stucchi Eusebio , Ribolini Adriano , Tognarelli Andrea
Published: July 2020

We aim at verifying whether the use of high-resolution coherency functionals could improve the signal-to-noise ratio (S/N) of Ground-Penetrating Radar data by introducing a variable and precisely picked velocity field in the migration process. After carrying out tests on synthetic data to schematically simulate the problem, assessing the types of functionals most suitable for GPR data analysis, we estimated a varying velocity field relative to a real dataset. This dataset was acquired in an archaeological area where an excavation after a GPR survey made it possible to define the position, type, and composition of the detected targets. Two functionals, the Complex Matched Coherency Measure and the Complex Matched Analysis, turned out to be effective in computing coherency maps characterized by high-resolution and strong noise rejection, where velocity picking can be done with high precision. By using the 2D velocity field thus obtained, migration algorithms performed better than in the case of constant or 1D velocity field, with satisfactory collapsing of the diffracted events and moving of the reflected energy in the correct position. The varying velocity field was estimated on different lines and used to migrate all the GPR profiles composing the survey covering the entire archaeological area. The time slices built with the migrated profiles resulted in a higher S/N than those obtained from non-migrated or migrated at constant velocity GPR profiles. The improvements are inherent to the resolution, continuity, and energy content of linear reflective areas. On the basis of our experience, we can state that the use of high-resolution coherency functionals leads to migrated GPR profiles with a high-grade of hyperbolas focusing. These profiles favor better imaging of the targets of interest, thereby allowing for a more reliable interpretation.
Watershed Modeling with Remotely Sensed Big Data: MODIS Leaf Area Index Improves Hydrology and Water Quality Predictions
Authors: Rajib Adnan , Kim I Luk , Golden Heather E. , Lane Charles R. , Kumar Sujay V. , Yu Zhiqiang , Jeyalakshmi Saranya
Published: July 2020

Traditional watershed modeling often overlooks the role of vegetation dynamics. There is also little quantitative evidence to suggest that increased physical realism of vegetation dynamics in process-based models improves hydrology and water quality predictions simultaneously. In this study, we applied a modified Soil and Water Assessment Tool (SWAT) to quantify the extent of improvements that the assimilation of remotely sensed Leaf Area Index (LAI) would convey to streamflow, soil moisture, and nitrate load simulations across a 16,860 km2 agricultural watershed in the midwestern United States. We modified the SWAT source code to automatically override the model’s built-in semiempirical LAI with spatially distributed and temporally continuous estimates from Moderate Resolution Imaging Spectroradiometer (MODIS). Compared to a “basic” traditional model with limited spatial information, our LAI assimilation model (i) significantly improved daily streamflow simulations during medium-to-low flow conditions, (ii) provided realistic spatial distributions of growing season soil moisture, and (iii) substantially reproduced the long-term observed variability of daily nitrate loads. Further analysis revealed that the overestimation or underestimation of LAI imparted a proportional cascading effect on how the model partitions hydrologic fluxes and nutrient pools. As such, assimilation of MODIS LAI data corrected the model’s LAI overestimation tendency, which led to a proportionally increased rootzone soil moisture and decreased plant nitrogen uptake. With these new findings, our study fills the existing knowledge gap regarding vegetation dynamics in watershed modeling and confirms that assimilation of MODIS LAI data in watershed models can effectively improve both hydrology and water quality predictions.