bluuebunny/crossref_metadata_2025 huggingface dataset:
Phosphoric Acid Modification of Hβ Zeolite for Guaiacol Hydrodeoxygenation
Authors: Wang Xun , Lv Yongkang , Zhu Shanhui , Wang Xuefeng , Deng Cunbao
Published: August 2021

Regulating the acid property of zeolite is an effective strategy to improve dehydration of intermediate alcohol, which is the rate-determining step in hydrodeoxygenation of lignin-based phenolic compounds. Herein, a commercial Hβ (SiO2/Al2O3 = 25) was modified by phosphoric acid, and evaluated in the catalytic performance of guaiacol to cyclohexane, combined with Ni/SiO2 prepared by the ammonia evaporation hydrothermal (AEH) method. Incorporating a small amount of phosphorus had little impact on the morphology, texture properties of Hβ, but led to dramatic variations in acid property, including the amount of acid sites and the ratio of Brønsted acid sites to Lewis acid sites, as confirmed by NH3-TPD, Py-IR, FT-IR and 27Al MAS NMR. Phosphorus modification on Hβ could effectively balance competitive adsorption of guaiacol on Lewis acid sites and intermediate alcohol dehydration on Brønsted acid sites, and then enhanced the catalytic performance of guaiacol hydrodeoxygenation to cyclohexane. By comparison, Hβ containing 2 wt.% phosphorus reached the highest activity and cyclohexane selectivity.
Hematopoietic Dysfunction during Graft-Versus-Host Disease: A Self-Destructive Process?
Authors: Müskens Konradin F. , Lindemans Caroline A. , Belderbos Mirjam E.
Published: August 2021

Graft-versus-host disease (GvHD) is a major complication of allogeneic hematopoietic (stem) cell transplantation (HCT). Clinically, GvHD is associated with severe and long-lasting hematopoietic dysfunction, which may contribute to the high mortality of GvHD after HCT. During GvHD, excessive immune activation damages both hematopoietic stem and progenitor cells and their surrounding bone marrow niche, leading to a reduction in cell number and functionality of both compartments. Hematopoietic dysfunction can be further aggravated by the occurrence—and treatment—of HCT-associated complications. These include immune suppressive therapy, coinciding infections and their treatment, and changes in the microbiome. In this review, we provide a structured overview of GvHD-mediated hematopoietic dysfunction, including the targets in the bone marrow, the mechanisms of action and the effect of GvHD-related complications and their treatment. This information may aid in the identification of treatment options to improve hematopoietic function in patients, during and after GvHD.
ERK Phosphorylation Regulates the Aml1/Runx1 Splice Variants and the TRP Channels Expression during the Differentiation of Glioma Stem Cell Lines
Authors: Santoni Giorgio , Nabissi Massimo , Amantini Consuelo , Santoni Matteo , Ricci-Vitiani Lucia , Pallini Roberto , Maggi Federica , Morelli Maria Beatrice
Published: August 2021

The identification of cancer stem cells in brain tumors paved the way for new therapeutic approaches. Recently, a role for the transcriptional factor Runx1/Aml1 and the downstream ion channel genes in brain cancer development and progression has been suggested. This study aimed to explore the expression and the role of Runx1/Aml1, its Aml1b and Aml1c splice variants and the downstream TRPA1 and TRPV1 ion channels in undifferentiated and day-14 differentiated neural stem cells (NSCs and D-NSCs) and glioblastoma stem cells (GSCs and D-GSCs) lines with different proneural (PN) or mesenchymal (MES) phenotype. Gene and protein expression were evaluated by qRT-PCR, cytofluorimetric, western blot and confocal microscopy analyses. Moreover, by western blot, we observed that ERK phosphorylation enhances the Aml1b and Aml1c protein expression during glioma differentiation. Furthermore, the agonists of TRPA1 and TRPV1 channels stimulated apoptosis/necrosis in GSCs and D-GSCs as evaluated by Annexin V and PI staining and cytofluorimetric analysis. Finally, by qRT-PCR, the modulation of Wnt/β catenin, FGF, and TGFβ/SMAD signaling pathways in PN- and MES-GSCs was reported. Overall, our results provide new evidence regarding Runx1/Aml1 isoform overexpression and modulation in TRP channel expression during gliomagenesis, thus offering new directions for glioblastoma therapy.
Transcriptional Landscape and Splicing Efficiency in Arabidopsis Mitochondria
Authors: Garcia Laura E. , Sanchez-Puerta M. Virginia
Published: August 2021

Plant mitochondrial transcription is initiated from multiple promoters without an apparent motif, which precludes their identification in other species based on sequence comparisons. Even though coding regions take up only a small fraction of plant mitochondrial genomes, deep RNAseq studies uncovered that these genomes are fully or nearly fully transcribed with significantly different RNA read depth across the genome. Transcriptomic analysis can be a powerful tool to understand the transcription process in diverse angiosperms, including the identification of potential promoters and co-transcribed genes or to study the efficiency of intron splicing. In this work, we analyzed the transcriptional landscape of the Arabidopsis mitochondrial genome (mtDNA) based on large-scale RNA sequencing data to evaluate the use of RNAseq to study those aspects of the transcription process. We found that about 98% of the Arabidopsis mtDNA is transcribed with highly different RNA read depth, which was elevated in known genes. The location of a sharp increase in RNA read depth upstream of genes matched the experimentally identified promoters. The continuously high RNA read depth across two adjacent genes agreed with the known co-transcribed units in Arabidopsis mitochondria. Most intron-containing genes showed a high splicing efficiency with no differences between cis and trans-spliced introns or between genes with distinct splicing mechanisms. Deep RNAseq analyses of diverse plant species will be valuable to recognize general and lineage-specific characteristics related to the mitochondrial transcription process.
Herb Sanqi-Derived Compound K Alleviates Oxidative Stress in Cultured Human Melanocytes and Improves Oxidative-Stress-Related Leukoderma in Guinea Pigs
Authors: Tang Suwei , Yang Lingli , Kuroda Yasutaka , Lai Sylvia , Xie Shaoqiong , Zhang Huimin , Katayama Ichiro
Published: August 2021

Sanqi, a traditional Chinese herb, is widely used for cardiovascular diseases, and its neuroprotective effects against oxidative stress were recently discovered. The purpose of this study was to investigate whether Sanqi-derived compound K (Sanqi-CK), an active metabolite of Sanqi, could protect melanocytes from oxidative stress. Cultured human primary skin epidermal melanocytes (HEMn-MPs) were treated with hydrogen peroxide (H2O2) in the presence or absence of Sanqi-CK. Sanqi-CK exhibited protective effects against H2O2-induced cell death by reducing oxidative stress. In addition, treatment with Sanqi-CK reversed the decreased glutathione reductase activity and decreased ratio of reduced glutathione (GSH)/oxidized glutathione (GSSG) seen in H2O2-treated melanocytes. Furthermore, topical application of Sanqi-CK alleviated leukoderma in guinea pigs, a disorder characterized by melanocyte cell death resulting from rhododendrol-induced oxidative stress. Taken together, these data suggest that Sanqi-CK protects melanocytes against oxidative stress, and its protective effects are associated with modulating the redox balance between GSH and GSSG and activating glutathione reductase. Thus, Sanqi-CK may be a good candidate for preventing melanocyte loss in oxidative-stress-associated pigmentary disorders.
Systemic Administration of PTH Supports Vascularization in Segmental Bone Defects Filled with Ceramic-Based Bone Graft Substitute
Authors: Freischmidt Holger , Armbruster Jonas , Bonner Emma , Guehring Thorsten , Nurjadi Dennis , Bechberger Maren , Sonntag Robert , Schmidmaier Gerhard , Grützner Paul Alfred , Helbig Lars
Published: August 2021

Non-unions continue to present a challenge to trauma surgeons, as current treatment options are limited, duration of treatment is long, and the outcome often unsatisfactory. Additionally, standard treatment with autologous bone grafts is associated with comorbidity at the donor site. Therefore, alternatives to autologous bone grafts and further therapeutic strategies to improve on the outcome and reduce cost for care providers are desirable. In this study in Sprague–Dawley rats we employed a recently established sequential defect model, which provides a platform to test new potential therapeutic strategies on non-unions while gaining mechanistic insight into their actions. The effects of a combinatorial treatment of a bone graft substitute (HACaS+G) implantation and systemic PTH administration was assessed by µ-CT, histological analysis, and bio-mechanical testing and compared to monotreatment and controls. Although neither PTH alone nor the combination of a bone graft substitute and PTH led to the formation of a stable union, our data demonstrate a clear osteoinductive and osteoconductive effect of the bone graft substitute. Additionally, PTH administration was shown to induce vascularization, both as a single adjuvant treatment and in combination with the bone graft substitute. Thus, systemic PTH administration is a potential synergistic co-treatment to bone graft substitutes.
Lysophosphatidic Acid Signaling in Cancer Cells: What Makes LPA So Special?
Authors: Balijepalli Pravita , Sitton Ciera C. , Meier Kathryn E.
Published: August 2021

Lysophosphatidic acid (LPA) refers to a family of simple phospholipids that act as ligands for G protein-coupled receptors. While LPA exerts effects throughout the body in normal physiological circumstances, its pathological role in cancer is of great interest from a therapeutic viewpoint. The numerous LPA receptors (LPARs) are coupled to a variety of G proteins, and more than one LPAR is typically expressed on any given cell. While the individual receptors signal through conventional GPCR pathways, LPA is particularly efficacious in stimulating cancer cell proliferation and migration. This review addresses the mechanistic aspects underlying these pro-tumorigenic effects. We provide examples of LPA signaling responses in various types of cancers, with an emphasis on those where roles have been identified for specific LPARs. While providing an overview of LPAR signaling, these examples also reveal gaps in our knowledge regarding the mechanisms of LPA action at the receptor level. The current understanding of the LPAR structure and the roles of LPAR interactions with other receptors are discussed. Overall, LPARs provide insight into the potential molecular mechanisms that underlie the ability of individual GPCRs (or combinations of GPCRs) to elicit a unique spectrum of responses from their agonist ligands. Further knowledge of these mechanisms will inform drug discovery, since GPCRs are promising therapeutic targets for cancer.
Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor
Authors: Arcadio Francesco , Zeni Luigi , Perri Chiara , D’Agostino Girolamo , Chiaretti Giudo , Porto Giovanni , Minardo Aldo , Cennamo Nunzio
Published: August 2021

Nowadays, the development of simple, fast, and low-cost selective sensors to detect substances of interest is of great importance in several application fields. Among this kind of sensors, those based on surface plasmon resonance (SPR) represent a promising category, since they are highly sensitive, versatile, and label-free. In this work, an SPR probe, based on a poly(methyl methacrylate) (PMMA) slab waveguide covered by a gold nanofilm, combined with a specific molecularly imprinted polymer (MIP) receptor for bovine serum albumin (BSA) protein, has been realized and experimentally characterized. The obtained experimental results have shown a limit of detection (LOD) equal to about 8.5 × 10−9 M. This value is smaller than the one achieved by another SPR probe, based on a D-shaped plastic optical fiber (POF), functionalized with the same MIP receptor; more specifically, the obtained LOD was reduced by about three orders of magnitude with respect to the POF configuration. Moreover, concerning the D-shaped POF configuration, no manufacturing process is present in the proposed sensor configuration. In addition, fibers are used only to connect the simple sensor chip with a light source and a detector, promoting a bio-chemical sensing approach based on disposable, low-cost, and removable chips.
Meditation Effects on Anxiety and Resilience of Preadolescents and Adolescents: A Randomized Controlled Study
Authors: Gomes Alexandra , Vieira dos Santos Joana , Vieira Luís Sérgio
Published: August 2021

Meditation has been described as having a positive impact on well-being while reducing anxiety and stress among those who practice, mainly working as a resource to cope with everyday difficulties. As a simple and easy to apply meditation technique, transcendental meditation (TM) has shown promising results in adults and in children, although more studies are needed to show the impact on psychological and behavioral dimensions in children and adolescents. This quasi-experimental, pre-test–post-test study, with a control group, aimed to evaluate the impact of TM on the stress and resilience of children and adolescents, with ages between 9 and 16 years old. Participants were selected within schools which implemented the Quiet Time Program (QT), from those who volunteered to participate. They were randomly assigned to an experimental group (immediate TM learning) and to a control group (delayed TM learning). A repeated measures ANOVA showed an interaction of time and group on externalizing behavior, from the strengths and difficulties measure. The experimental group decreased on externalizing less adjusted behaviors, while the control group increased in this aspect, after a twelve-week period. TM failed to reduce anxiety and to contribute to resilience in the TM experimental group. Both groups improved anxiety indicators. The results might suggest students were acting upon their expectation of improvement on practicing TM or solely modifying their behavior along the contextual factors, which affected both groups equally.
Adaptive Heritage: Is This Creative Thinking or Abandoning Our Values?
Authors: Perry Jim , Gordon Iain J.
Published: August 2021

Protected areas, such as natural World Heritage sites, RAMSAR wetlands and Biosphere Reserves, are ecosystems within landscapes. Each site meets certain criteria that allow it to qualify as a heritage or protected area. Both climate change and human influence (e.g., incursion, increased tourist visitation) are altering biophysical conditions at many such sites. As a result, conditions at many sites are falling outside the criteria for their original designation. The alternatives are to change the criteria, remove protection from the site, change site boundaries such that the larger or smaller landscape meets the criteria, or manage the existing landscape in some way that reduces the threat. This paper argues for adaptive heritage, an approach that explicitly recognizes changing conditions and societal value. We discuss the need to view heritage areas as parts of a larger landscape, and to take an adaptive approach to the management of that landscape. We offer five themes of adaptive heritage: (1) treat sites as living heritage, (2) employ innovative governance, (3) embrace transparency and accountability, (4) invest in monitoring and evaluation, and (5) manage adaptively. We offer the Australian Wet Tropics as an example where aspects of adaptive heritage currently are practiced, highlighting the tools being used. This paper offers guidance supporting decisions about natural heritage in the face of climate change and non-climatic pressures. Rather than delisting or lowering standards, we argue for adaptive approaches.
Ancient Faunal History Revealed by Interdisciplinary Biomolecular Approaches
Authors: Rosengren Erika , Acatrinei Arina , Cruceru Nicolae , Dehasque Marianne , Haliuc Aritina , Lord Edana , Mircea Cristina I. , Rusu Ioana , Mármol-Sánchez Emilio , Kelemen Beatrice S. , Meleg Ioana N.
Published: August 2021

Starting four decades ago, studies have examined the ecology and evolutionary dynamics of populations and species using short mitochondrial DNA fragments and stable isotopes. Through technological and analytical advances, the methods and biomolecules at our disposal have increased significantly to now include lipids, whole genomes, proteomes, and even epigenomes. At an unprecedented resolution, the study of ancient biomolecules has made it possible for us to disentangle the complex processes that shaped the ancient faunal diversity across millennia, with the potential to aid in implicating probable causes of species extinction and how humans impacted the genetics and ecology of wild and domestic species. However, even now, few studies explore interdisciplinary biomolecular approaches to reveal ancient faunal diversity dynamics in relation to environmental and anthropogenic impact. This review will approach how biomolecules have been implemented in a broad variety of topics and species, from the extinct Pleistocene megafauna to ancient wild and domestic stocks, as well as how their future use has the potential to offer an enhanced understanding of drivers of past faunal diversity on Earth.
Mesonephric-like Differentiation of Endometrial Endometrioid Carcinoma: Clinicopathological and Molecular Characteristics Distinct from Those of Uterine Mesonephric-like Adenocarcinoma
Authors: Park Sujin , Bae Go Eun , Kim Jiyoung , Kim Hyun-Soo
Published: August 2021

When diagnosing endometrial carcinoma cases, we encountered histological features that strikingly resembled uterine mesonephric-like adenocarcinoma (MLA), but the differential diagnosis remained challenging after performing immunostaining. Considering the aggressive biological behavior and poor prognosis of uterine MLA, we believe that the accurate recognition of mesonephric-like differentiation (MLD) is important in the diagnosis of endometrial carcinoma. We aimed to investigate the clinicopathological and molecular characteristics of such cases and compared them with those of uterine MLAs. Five patients diagnosed with endometrioid carcinoma (EC) with MLD were included in this study. Histological evaluation, immunostaining, and targeted sequencing were performed. All five tumors showed typical morphological features of MLA, including densely aggregated tubular structures, deep basophilia under low-power magnification microscopy, eosinophilic intraluminal secretions, and diverse growth patterns. Immunostaining revealed moderate-to-strong nuclear immunoreactivity for estrogen and progesterone receptors in more than 50% tumor cells. The staining intensities and proportions of PAX2 and GATA3 were variable. None of the tumors harbored KRAS mutations. Considering the prognostic implications, ancillary tests, including immunostaining and targeted sequencing, should be performed to accurately differentiate between endometrial EC-MLD and uterine MLA.
Hospitalist Perceptions of Barriers to Lung Ultrasound Adoption in Diverse Hospital Environments
Authors: Maw Anna M. , Ho P. Michael , Morris Megan A. , Glasgow Russell E. , Huebschmann Amy G. , Barnard Juliana G. , Metter Robert , Tierney David M. , Mathews Benji K. , Havranek Edward P. , Kissler Mark , Fleshner Michelle , Burian Barbara K. , Platz Elke , Soni Nilam J.
Published: August 2021

Despite the many advantages of lung ultrasound (LUS) in the diagnosis and management of patients with dyspnea, its adoption among hospitalists has been slow. We performed semi-structured interviews of hospitals from four diverse health systems in the United States to understand determinants of adoption within a range of clinical settings. We used the diffusion of innovation theory to guide a framework analysis of the data. Of the 27 hospitalists invited, we performed 22 interviews from four hospitals of diverse types. Median years post-residency of interviewees was 10.5 [IQR:5-15]. Four main themes emerged: (1) There are important clinical advantages to LUS despite operator dependence, (2) LUS enhances patient and clinician experience, (3) Investment of clinician time to learn and perform LUS is a barrier to adoption but yields improved efficiency for the health system and (4) Mandated training and use may be necessary to achieve broad adoption as monetary incentives are less effective. Despite the perceived benefits of LUS for patients, clinicians and health systems, a significant barrier to broad LUS adoption is the experience of time scarcity by hospitalists. Future implementation strategies should focus on changes to the clinical environment that address clinician barriers to learning and adoption of new skills.
Estimation of Winter Wheat Yield from UAV-Based Multi-Temporal Imagery Using Crop Allometric Relationship and SAFY Model
Authors: Song Yang , Wang Jinfei , Shan Bo
Published: August 2021

Crop yield prediction and estimation play essential roles in the precision crop management system. The Simple Algorithm for Yield Estimation (SAFY) has been applied to Unmanned Aerial Vehicle (UAV)-based data to provide high spatial yield prediction and estimation for winter wheat. However, this crop model relies on the relationship between crop leaf weight and biomass, which only considers the contribution of leaves on the final biomass and yield calculation. This study developed the modified SAFY-height model by incorporating an allometric relationship between ground-based measured crop height and biomass. A piecewise linear regression model is used to establish the relationship between crop height and biomass. The parameters of the modified SAFY-height model are calibrated using ground measurements. Then, the calibrated modified SAFY-height model is applied on the UAV-based photogrammetric point cloud derived crop height and effective leaf area index (LAIe) maps to predict winter wheat yield. The growing accumulated temperature turning points of an allometric relationship between crop height and biomass is 712 °C. The modified SAFY-height model, relative to traditional SAFY, provided more accurate yield estimation for areas with LAI higher than 1.01 m2/m2. The RMSE and RRMSE are improved by 3.3% and 0.5%, respectively.
Short-Term Electric Load Forecasting Based on Variational Mode Decomposition and Grey Wolf Optimization
Authors: Zhou Mengran , Hu Tianyu , Bian Kai , Lai Wenhao , Hu Feng , Hamrani Oumaima , Zhu Ziwei
Published: August 2021

Short-term electric load forecasting plays a significant role in the safe and stable operation of the power system and power market transactions. In recent years, with the development of new energy sources, more and more sources have been integrated into the grid. This has posed a serious challenge to short-term electric load forecasting. Focusing on load series with non-linear and time-varying characteristics, an approach to short-term electric load forecasting using a “decomposition and ensemble” framework is proposed in this paper. The method is verified using hourly load data from Oslo and the surrounding areas of Norway. First, the load series is decomposed into five components by variational mode decomposition (VMD). Second, a support vector regression (SVR) forecasting model is established for the five components to predict the electric load components, and the grey wolf optimization (GWO) algorithm is used to optimize the cost and gamma parameters of SVR. Finally, the predicted values of the five components are superimposed to obtain the final electric load forecasting results. In this paper, the proposed method is compared with GWO-SVR without modal decomposition and using empirical mode decomposition (EMD) to test the impact of VMD on prediction. This paper also compares the proposed method with the SVR model using VMD and other optimization algorithms. The four evaluation indexes of the proposed method are optimal: MAE is 71.65 MW, MAPE is 1.41%, MSE is 10,461.32, and R2 is 0.9834. This indicates that the proposed method has a good application prospect for short-term electric load forecasting.
A Review of the State of the Art of Biomethane Production: Recent Advancements and Integration of Renewable Energies
Authors: Calise Francesco , Cappiello Francesco Liberato , Cimmino Luca , d’Accadia Massimo Dentice , Vicidomini Maria
Published: August 2021

Anaerobic Digestion (AD) is a well-established process that is becoming increasingly popular, especially as a technology for organic waste treatment; the process produces biogas, which can be upgraded to biomethane, which can be used in the transport sector or injected into the natural gas grid. Considering the sensitivity of Anaerobic Digestion to several process parameters, mathematical modeling and numerical simulations can be useful to improve both design and control of the process. Therefore, several different modeling approaches were presented in the literature, aiming at providing suitable tools for the design and simulation of these systems. The purpose of this study is to analyze the recent advancements in the biomethane production from different points of view. Special attention is paid to the integration of this technology with additional renewable energy sources, such as solar, geothermal and wind, aimed at achieving a fully renewable biomethane production. In this case, auxiliary heat may be provided by solar thermal or geothermal energy, while wind or photovoltaic plants can provide auxiliary electricity. Recent advancements in plants design, biomethane production and mathematical modeling are shown in the paper, and the main challenges that these fields must face with are discussed. Considering the increasing interest of industries, public policy makers and researchers in this field, the efficiency and profitability such hybrid renewable solutions for biomethane production are expected to significantly improve in the next future, provided that suitable subsidies and funding policies are implemented to support their development.
Catalytic Hydrogen Combustion for Domestic and Safety Applications: A Critical Review of Catalyst Materials and Technologies
Authors: Kozhukhova Alina E. , du Preez Stephanus P. , Bessarabov Dmitri G.
Published: August 2021

Spatial heating and cooking account for a significant fraction of global domestic energy consumption. It is therefore likely that hydrogen combustion will form part of a hydrogen-based energy economy. Catalytic hydrogen combustion (CHC) is considered a promising technology for this purpose. CHC is an exothermic reaction, with water as the only by-product. Compared to direct flame-based hydrogen combustion, CHC is relatively safe as it foregoes COx, CH4, and under certain conditions NOx formation. More so, the risk of blow-off (flame extinguished due to the high fuel flow speed required for H2 combustion) is adverted. CHC is, however, perplexed by the occurrence of hotspots, which are defined as areas where the localized surface temperature is higher than the average surface temperature over the catalyst surface. Hotspots may result in hydrogen’s autoignition and accelerated catalyst degradation. In this review, catalyst materials along with the hydrogen technologies investigated for CHC applications were discussed. We showed that although significant research has been dedicated to CHC, relatively limited commercial applications have been identified up to date. We further showed the effect of catalyst support selection on the performance and durability of CHC catalysts, as well as a holistic summary of existing catalysts used for various CHC applications and catalytic burners. Lastly, the relevance of CHC applications for safety purposes was demonstrated.
Heat Transfer Performance Potential with a High-Temperature Phase Change Dispersion
Authors: Fischer Ludger , Mura Ernesto , O’Neill Poppy , von Arx Silvan , Worlitschek Jörg , Qiao Geng , Li Qi , Ding Yulong
Published: August 2021

Phase change dispersions are useful for isothermal cooling applications. As a result of the phase changes that occur in PCDs, they are expected to have greater storage capacities than those of single-phase heat transfer fluids. However, for appropriate heat exchanger dimensions and geometries for use in phase change dispersions, knowledge about the convective heat transfer coefficients of phase change dispersions is necessary. A test unit for measuring the local heat transfer coefficients and Nusselt numbers of PCDs was created. The boundary condition of constant heat flux was chosen for testing, and the experimental heat transfer coefficients and Nusselt numbers for the investigated phase change dispersion were established. Different experimental parameters, such as the electrical wall heat input, Reynolds number, and mass flow rate, were varied during testing, and the results were compared to those of water tests. It was found that, due to the tendency of low-temperature increases in phase change dispersions, the driving temperature difference is greater than that of water. In addition, larger heat storage capacities were obtained for phase change dispersions than for water. Through this experimentation, it was acknowledged that future investigation into the optimised operating conditions must be performed.
Adaptive Impedance Control of a Novel Automated Umbilical System for Propellant Loading
Authors: Tao Han , Cong Dacheng
Published: August 2021

In this paper, an automated umbilical system based on a 6-dof (degree of freedom) hydraulic parallel mechanism is proposed to automate the rocket propellant loading process. The mechanical structure, vision acquisition algorithm, and control algorithm used in the system are described in detail in the paper. To address the fluid nonlinearity problem of the hydraulic drive system, nonlinear compensation and three-state feedback control are used in the paper to enhance the performance of the hydraulic system. For the problem of force tracking during the docking process between the umbilical system and the rocket, an adaptive impedance control algorithm based on the online environmental parameter estimation is proposed in the paper, which effectively reduces the contact force during the docking process. The dynamic tracking and docking experiments indicate that this automated umbilical system features rapid reaction speed, high measurement precision, and good flexibility, which can be used to realize the auto-mating and following task for the propellant loading robot in a hazardous environment.
Analysis of Dynamic Characteristics and Power Losses of High Speed on/off Valve with Pre-Existing Control Algorithm
Authors: Zhong Qi , Wang Xiele , Xie Geng , Yang Huayong , Yu Cheng , Xu Enguang , Li Yanbiao
Published: August 2021

A high-speed on/off valve (HSV) is generally the core component of a digital hydraulic transmission system. Therefore, its dynamic characteristics often restrict the overall performance of the digital hydraulic system. Most of the current studies focus on the optimization on the dynamic characteristics or the energy characteristics, few studies have comprehensively considered the two characteristics of the valve together. In this paper, a pre-existing control algorithm (PECA) is proposed to improve the dynamic characteristics of the HSV, and simultaneously optimize the power losses of the HSV to improve its energy conversion efficiency. The results show that, compared with the traditional single-voltage driven strategy, the opening time of the PECA decreases by 29.4%, the closing time decreases by 59.6%, and the energy conversion rate increases by 7.9%.
Production Profile of Farms and Methane and Nitrous Oxide Emissions
Authors: Koloszko-Chomentowska Zofia , Sieczko Leszek , Trochimczuk Roman
Published: August 2021

The negative impact of agricultural production on the environment is manifested, above all, in the emission of greenhouse gases (GHG). The goals of this study were to estimate methane and nitrous oxide emissions at the level of individual farms and indicate differences in emissions depending on the type of production, and to investigate dependencies between greenhouse gas emissions and economic indicators. Methane and nitrous oxide emissions were estimated at three types of farms in Poland, based on FADN data: field crops, milk, and mixed. Data were from 2004–2018. Statistical analysis confirmed the relationship between greenhouse gas emissions and economic performance. On milk farms, the value of methane and nitrous oxide emissions increased with increased net value added and farm income. Milk farms reached the highest land productivity and the highest level of income per 1 ha of farmland. On field crops farms, the relationship between net value added and farm income and methane and nitrous oxide emissions was negative. Animals remain a strong determinant of methane and nitrous oxide emissions, and the emissions at milk farms were the highest. On mixed farms, emissions result from intensive livestock and crop production. In farms of the field crops type, emissions were the lowest and mainly concerned crops.
Accurate Computation of Mutual Inductance of Non Coaxial Pancake Coils
Authors: Parise Mauro , Loreto Fabrizio , Romano Daniele , Antonini Giulio , Ekman Jonas
Published: August 2021

The computation of self and mutual inductances of coils is a classic problem of electrical engineering. The accurate modeling of coupled coils has received renewed interest with the spread of wireless power transfer systems. This problem has been quite well addressed for coplanar or perfectly coaxial coils but it is known that the misalignment conditions easily lead to a sharp decrease in the efficiency. Hence, it is crucial to take misalignment into account in order to properly design the overall wireless power transfer system. This work presents a study to compute analytically the mutual inductance of non-coaxial pancake coils with parallel axes. The accuracy of the proposed methodology is tested by comparison with the numerical results obtained using the tool Fast-Henry. Then, a wireless power transfer system, comprising a full bridge inverter is considered, showing the impact of the misalignment on the coupling between two pancake coils and, thus, between the source and the load.
Electric Field and Temperature Simulations of High-Voltage Direct Current Cables Considering the Soil Environment
Authors: Jörgens Christoph , Clemens Markus
Published: August 2021

For long distance electric power transport, high-voltage direct current (HVDC) cable systems are a commonly used solution. Space charges accumulate in the HVDC cable insulations due to the applied voltage and the nonlinear electric conductivity of the insulation material. The resulting electric field depends on the material parameters of the surrounding soil environment that may differ locally and have an influence on the temperature distribution in the cable and the environment. To use the radial symmetry of the cable geometry, typical electric field simulations neglect the influence of the surrounding soil, due to different dimensions of the cable and the environment and the resulting high computational effort. Here, the environment and its effect on the resulting electric field is considered and the assumption of a possible radial symmetric temperature within the insulation is analyzed. To reduce the computation time, weakly coupled simulations are performed to compute the temperature and the electric field inside the cable insulation, neglecting insulation losses. The results of a weakly coupled simulation are compared against those of a full transient simulation, considering the insulation losses for two common cable insulations with different maximum operation temperatures. Due to the buried depth of HV cables, an approximately radial symmetric temperature distribution within the insulation is obtained for a single cable and cable pairs when, considering a metallic sheath. Furthermore, the simulations show a temperature increase of the earth–air interface above the buried cable that needs to be considered when computing the cable conductor temperature, using the IEC standards.
Organic Wastes Amended with Sorbents Reduce N2O Emissions from Sugarcane Cropping
Authors: Westermann Maren , Brackin Richard , Robinson Nicole , Salazar Cajas Monica , Buckley Scott , Bailey Taleta , Redding Matthew , Kochanek Jitka , Hill Jaye , Guillou Stéphane , Freitas Joao Carlos Martins , Wang Weijin , Pratt Chris , Fujinuma Ryo , Schmidt Susanne
Published: August 2021

Nutrient-rich organic wastes and soil ameliorants can benefit crop performance and soil health but can also prevent crop nutrient sufficiency or increase greenhouse gas emissions. We hypothesised that nitrogen (N)-rich agricultural waste (poultry litter) amended with sorbents (bentonite clay or biochar) or compost (high C/N ratio) attenuates the concentration of inorganic nitrogen (N) in soil and reduces emissions of nitrous oxide (N2O). We tested this hypothesis with a field experiment conducted on a commercial sugarcane farm, using in vitro incubations. Treatments received 160 kg N ha−1, either from mineral fertiliser or poultry litter, with additional N (2–60 kg N ha−1) supplied by the sorbents and compost. Crop yield was similar in all N treatments, indicating N sufficiency, with the poultry litter + biochar treatment statistically matching the yield of the no-N control. Confirming our hypothesis, mineral N fertiliser resulted in the highest concentrations of soil inorganic N, followed by poultry litter and the amended poultry formulations. Reflecting the soil inorganic N concentrations, the average N2O emission factors ranked as per the following: mineral fertiliser 8.02% > poultry litter 6.77% > poultry litter + compost 6.75% > poultry litter + bentonite 5.5% > poultry litter + biochar 3.4%. All emission factors exceeded the IPCC Tier 1 default for managed soils (1%) and the Australian Government default for sugarcane soil (1.25%). Our findings reinforce concerns that current default emissions factors underestimate N2O emissions. The laboratory incubations broadly matched the field N2O emissions, indicating that in vitro testing is a cost-effective first step to guide the blending of organic wastes in a way that ensures N sufficiency for crops but minimises N losses. We conclude that suitable sorbent-waste formulations that attenuate N release will advance N efficiency and the circular nutrient economy.
Long-Term Projection of Species-Specific Responses to Chronic Additions of Nitrogen, Sulfur, and Lime
Authors: Storm Alexander , Adams Mary Beth , Schuler Jamie
Published: August 2021

Elevated acid deposition has been a concern in the central Appalachian region for decades. A long-term acidification experiment on the Fernow Experimental Forest in central West Virginia was initiated in 1996 and continues to this day. Ammonium sulfate was used to simulate elevated acid deposition. A concurrent lime treatment with an ammonium sulfate treatment was also implemented to assess the ameliorative effects of base cations to offset acidification. We show that the forest vegetation simulator growth model can be locally calibrated and used to project stand growth and development over 40 years to assess the impacts of acid deposition and liming. Modeled projections showed that pin cherry (initially) and sweet birch responded positively to nitrogen and sulfur additions, while black cherry, red maple, and cucumbertree responded positively to nitrogen, sulfur, and lime. Yellow-poplar negatively responded to both treatments. Despite these differences, our projections show a maximum of 5% difference in total stand volume among treatments after 40 years.
A Novel Plant Resistance Inducer for the Protection of European Ash (Fraxinus excelsior L.) against Hymenoscyphus fraxineus—Preliminary Studies
Authors: Turczański Krzysztof , Bełka Marta , Kukawka Rafal , Spychalski Maciej , Smiglak Marcin
Published: August 2021

Ash tree disease is caused by an ascomycete fungus Hymenoscyphus fraxineus, which first emerged in 1992, eastern Poland. Site factors, genetic predispositions, and resistance to the pathogen have not been fully described yet. The general aim of the study undertaken was to check the effect of using a new active substance representing benzothiadiazoles, a BTH derivative, namely, N-methyl-N-methoxyamide-7-carboxybenzo(1.2.3)thiadiazole (BTHWA), on ash saplings. A total of 41 ash saplings, aged three to five years, were subjected to this experiment in six variants of treatment. The results of the inoculation with H. fraxineus indicated that the treatment with BTHWA resulted in the limitation of the size of necrotic phloem lesions. Although the lesions were detectable in the cross section, the plants showed no visible signs of infection. The results suggest that H. fraxineus development in ash saplings can be slowed down or even completely stopped through triggering plant resistance by BTHWA.
Selection Process of a Mixed Inoculum of Non-Saccharomyces Yeasts Isolated in the D.O.Ca. Rioja
Authors: Escribano-Viana Rocío , González-Arenzana Lucía , Garijo Patrocinio , López Rosa , Santamaría Pilar , Gutiérrez Ana Rosa
Published: August 2021

The use of non-Saccharomyces yeasts in sequential fermentations with S. cerevisiae has been proposed to improve the organoleptic characteristics involved in the quality of wine. The present study set out to select a non-Saccharomyces inoculum from the D.O.Ca. Rioja for use in winemaking. Strains included in the study belonged to Torulaspora delbrueckii, Lachancea thermotolerans, Metschnikowia pulcherrima, Zygosaccharomyces bailii, Williopsis pratensis, Debaryomyces hansenii, Pichia kluyveri, Sporidiobolus salmonicolor, Candida spp., Cryptococcus spp. and two mixed inocula of Lachancea thermotolerans-Torulaspora delbrueckii in a 30/70 ratio. In the first stage of the process, SO2 resistance and presence of enzymatic activities related to wine aroma and wine color and fining (esterase, esterase-lipase, lipase, leucine arylamidase, valine arylamidase, cystine arylamidase, β-glucosidase, pectinase, cellulose, xylanase and glucanase) were studied. In the later stages, selection criteria such as fermentative behavior, aroma compound production or influence on phenolic compounds were studied in laboratory scale vinifications. Taking into account the results obtained in the different stages of the process, a mixed inoculum of Lachancea thermotolerans-Torulaspora delbrueckii in a 30/70 ratio was finally selected. This inoculum stood out for its high implantation capacity, the production of compounds of interest such as glycerol and lactic acid and the consequent modulation of wine acidity. Given these characteristics, the selected inoculum is suitable for the production of quality wines.
Physico-Chemical Characteristics and Amino Acid Content Evaluation of Citric Acid by-Product Produced by Microbial Fermentation as a Potential Use in Animal Feed
Authors: Oryza.S Mutyarsih , Wongtangtintharn Sawitree , Tengjaroenkul Bundit , Cherdthong Anusorn , Tanpong Sirisak , Bunchalee Pasakorn , Pootthachaya Padsakorn , Reungsang Alissara , Polyorach Sineenart
Published: August 2021

The production of citric acid produces 70% waste product or by-product. This by-product is produced by microbial fermentation which could be used as an alternative raw material for animal feed because it still contains citric acid, which could help to reduce pathogenic bacteria. The objective of this study is to evaluate the physical and chemical value of citric acid by-product from rice (CABR) to compare the properties with those of rice bran and broken rice and to determine its potential as an alternative energy source in animal feed. The chemical composition of CABR was calculated using proximate analysis. The color of CABR was darker, and the bulk density value was 549.65 (g/L) (p < 0.05). With free flow, the angle of repose was 40°, and the particle size had less polygonal starch granules. CABR had a low pH of 4.77 and contained 19.80% crude protein, 11.97% crude fiber, and 4005.72 kcal/kg of energy. CABR had a higher crude protein value than broken rice and rice bran and a higher gross energy value than broken rice but less than rice bran. It also had a higher crude fiber value (p > 0.05). The results suggest that CABR could be utilized as an energy and protein source for animal feed formulations.
Fishes Receives Its First Impact Factor
Authors: Esteban Maria Angeles , Baldisserotto Bernardo , Hallerman Eric
Published: August 2021

“A new journey for Fishes” [...]
The Bioaccessibility of Phenolics, Flavonoids, Carotenoids, and Capsaicinoid Compounds: A Comparative Study of Cooked Potato Cultivars Mixed with Roasted Pepper Varieties
Authors: Hamed Mansor , Holm David G. , Bartolo Michael , Raigond Pinky , Sathuvalli Vidyasagar , Jayanty Sastry S.
Published: August 2021

An in vitro method was used to assess the bioaccessibility of phenolics, flavonoids, carotenoids, and capsaicinoid compounds in different cooked potatoes mixed with roasted peppers (Capsicum annuum), Joe Parker (JP, hot), and Sweet Delilah (SD, sweet). The present study identified differences in the bioaccessibility of bioactive compounds among the potato cultivars (Solanum tuberosum) Purple Majesty (PM; purple flesh), Yukon Gold (YG; yellow flesh), Rio Grande Russet (RG; white flesh) and a numbered selection (CO 97226-2R/R (R/R; red flesh)). The bioactive compounds and capsaicinoid compounds in potatoes and peppers were estimated before and after in vitro digestion. Before digestion, the total phenolic content of potato cultivars mixed with JP was in the following order: R/R > PM > YG > RG. The highest levels of carotenoids were 194.34 µg/g in YG and 42.92 µg/g in the RG cultivar when mixed with roasted JP. The results indicate that the amount of bioaccessible phenolics ranged from 485 to 252 µg/g in potato cultivars mixed with roasted JP. The bioaccessibility of flavonoids ranged from 185.1 to 59.25 µg/g. The results indicate that the YG cultivar mixed with JP and SD showed the highest phenolic and carotenoid bioaccessibility. In contrast, the PM mixed with JP and SD contained the lowest phenolic and carotenoid bioaccessibility. Our results indicate that the highest flavonoid bioaccessibility occurred in R/R mixed with roasted JP and SD. The lowest flavonoids bioaccessibility occurred in PM and the RG. The maximum bioaccessible amount of capsaicin was observed in YG mixed with JP, while the minimum bioaccessibility was observed with PM.
Investigation and Rapid Discrimination of Food-Related Bacteria under Stress Treatments Using IR Microspectroscopy
Authors: Klein Daniel , Breuch René , Reinmüller Jessica , Engelhard Carsten , Kaul Peter
Published: August 2021

Because the robust and rapid determination of spoilage microorganisms is becoming increasingly important in industry, the use of IR microspectroscopy, and the establishment of robust and versatile chemometric models for data processing and classification, is gaining importance. To further improve the chemometric models, bacterial stress responses were induced, to study the effect on the IR spectra and to improve the chemometric model. Thus, in this work, nine important food-relevant microorganisms were subjected to eight stress conditions, besides the regular culturing as a reference. Spectral changes compared to normal growth conditions without stressors were found in the spectral regions of 900–1500 cm−1 and 1500–1700 cm−1. These differences might stem from changes in the protein secondary structure, exopolymer production, and concentration of nucleic acids, lipids, and polysaccharides. As a result, a model for the discrimination of the studied microorganisms at the genus, species and strain level was established, with an accuracy of 96.6%. This was achieved despite the inclusion of various stress conditions and times after incubation of the bacteria. In addition, a model was developed for each individual microorganism, to separate each stress condition or regular treatment with 100% accuracy.