bluuebunny/crossref_metadata_2025 huggingface dataset:
Oxidative Phosphorylation Dysfunction Modifies the Cell Secretome
Authors: Garrido-Pérez Nuria , Vela-Sebastián Ana , López-Gallardo Ester , Emperador Sonia , Iglesias Eldris , Meade Patricia , Jiménez-Mallebrera Cecilia , Montoya Julio , Bayona-Bafaluy M. Pilar , Ruiz-Pesini Eduardo
Published: May 2020

Mitochondrial oxidative phosphorylation disorders are extremely heterogeneous conditions. Their clinical and genetic variability makes the identification of reliable and specific biomarkers very challenging. Until now, only a few studies have focused on the effect of a defective oxidative phosphorylation functioning on the cell’s secretome, although it could be a promising approach for the identification and pre-selection of potential circulating biomarkers for mitochondrial diseases. Here, we review the insights obtained from secretome studies with regard to oxidative phosphorylation dysfunction, and the biomarkers that appear, so far, to be promising to identify mitochondrial diseases. We propose two new biomarkers to be taken into account in future diagnostic trials.
Akkermansia muciniphila Aspartic Protease Amuc_1434* Inhibits Human Colorectal Cancer LS174T Cell Viability via TRAIL-Mediated Apoptosis Pathway
Authors: Meng Xin , Zhang Jinrui , Wu Hao , Yu Dahai , Fang Xuexun
Published: May 2020

Mucin2 (Muc2) is the main component of the intestinal mucosal layer and is highly expressed in mucous colorectal cancer. Previous studies conducted by our lab found that the recombinant protein Amuc_1434 (expressed in Escherichia coli prokaryote cell system, hereinafter termed Amuc_1434*), derived from Akkermansia muciniphila, can degrade Muc2. Thus, the main objective of this study was to explore the effects of Amuc_1434* on LS174T in colorectal cancer cells expressing Muc2. Results from this study demonstrated that Amuc_1434* inhibited the proliferation of LS174T cells, which was related to its ability to degrade Muc2. Amuc_1434* also blocked the G0/G1 phase of the cell cycle of LS174T cells and upregulated the expression of tumor protein 53 (p53), which is a cell cycle-related protein. In addition, Amuc_1434* promoted apoptosis of LS174T cells and increased mitochondrial ROS levels in LS174T cells. The mitochondrial membrane potential of LS174T cells was also downregulated by Amuc_1434*. Amuc_1434* can activate the death receptor pathway and mitochondrial pathway of apoptosis by upregulating tumor-necrosis-factor-related apoptosis-inducing ligand (TRAIL). In conclusion, our study was the first to demonstrate that the protein Amuc_1434* derived from Akkermansia muciniphila suppresses LS174T cell viability via TRAIL-mediated apoptosis pathway.
Different Roles of Auxins in Somatic Embryogenesis Efficiency in Two Picea Species
Authors: Hazubska-Przybył Teresa , Ratajczak Ewelina , Obarska Agata , Pers-Kamczyc Emilia
Published: May 2020

The effects of auxins 2,4-D (2,4-dichlorophenoxyacetic acid), NAA (1-naphthaleneacetic acid) or picloram (4-amino-3,5,6-trichloropicolinic acid; 9 µM) and cytokinin BA (benzyloadenine; 4.5 µM) applied in the early stages of somatic embryogenesis (SE) on specific stages of SE in Picea abies and P. omorika were investigated. The highest SE initiation frequency was obtained after 2,4-D application in P. omorika (22.00%) and picloram application in P. abies (10.48%). NAA treatment significantly promoted embryogenic tissue (ET) proliferation in P. abies, while 2,4-D treatment reduced it. This reduction was related to the oxidative stress level, which was lower with the presence of NAA in the proliferation medium and higher with the presence of 2,4-D. The reduced oxidative stress level after NAA treatment suggests that hydrogen peroxide (H2O2) acts as a signalling molecule and promotes ET proliferation. NAA and picloram in the proliferation medium decreased the further production and maturation of P. omorika somatic embryos compared with that under 2,4-D. The quality of the germinated P. abies embryos and their development into plantlets depended on the auxin type and were the highest in NAA-originated embryos. These results show that different auxin types can generate different physiological responses in plant materials during SE in both spruce species.
Fabrication of Mechanically Reinforced Gelatin/Hydroxyapatite Bio-Composite Scaffolds by Core/Shell Nozzle Printing for Bone Tissue Engineering
Authors: Kim Haeri , Hwangbo Hanjun , Koo YoungWon , Kim GeunHyung
Published: May 2020

In tissue engineering, biocompatible scaffolds are used as 3D cell niches to provide a similar environment to that of native tissue for seeded cells to regenerate the target tissue. When engineering bone tissue, high mechanical strength and calcium phosphate composition are essential factors to consider. In this study, we fabricated biocompatible composite scaffolds composed of synthetic polymers (polycaprolactone (PCL) and poly (vinyl alcohol) (PVA)), natural polymers (gelatin and collagen) and bioceramic (hydroxyapatite; HA) for bone tissue engineering. The synthetic polymers were used to enhance the mechanical properties of the composite scaffolds while the natural protein-based polymers were used to enhance various cellular activities, such as cell adhesion and proliferation. Meanwhile, the bioceramic was introduced to promote osteogenic differentiation. Composite scaffolds were evaluated for their physical characteristics, such as mechanical, swelling and protein absorbing properties as well as biological properties (cell proliferation, alkaline phosphatase (ALP) activities and calcium deposition) with human osteoblast-like cells (MG63). Consequently, incorporation of hydroxyapatite into the gelatin/PVA (C-GPH) scaffold showed 5-fold and 1.5-fold increase in calcium deposition and ALP activities, respectively compared to gelatin/PVA scaffold (C-GP). Moreover, compressive modulus also increased 1.8-fold. Integration of PCL core into gelatin/PVA/hydroxyapatite scaffold (C-PGPH) further amplified the compressive modulus 1.5-fold. In conclusion, the scaffold that is reinforced with HA particles and integrated with PCL core of the struts showed significant potential in field of bone tissue engineering.
A New Approach to Keep the Privacy Information of the Signer in a Digital Signature Scheme
Authors: Duong Dung Hoang , Susilo Willy , Trinh Viet Cuong
Published: May 2020

In modern applications, such as Electronic Voting, e-Health, e-Cash, there is a need that the validity of a signature should be verified by only one responsible person. This is opposite to the traditional digital signature scheme where anybody can verify a signature. There have been several solutions for this problem, the first one is we combine a signature scheme with an encryption scheme; the second one is to use the group signature; and the last one is to use the strong designated verifier signature scheme with the undeniable property. In this paper, we extend the traditional digital signature scheme to propose a new solution for the aforementioned problem. Our extension is in the sense that only a designated verifier (responsible person) can verify a signer’s signature, and if necessary (in case the signer refuses to admit his/her signature) the designated verifier without revealing his/her secret key is able to prove to anybody that the signer has actually generated the signature. The comparison between our proposed solution and the three existing solutions shows that our proposed solution is the best one in terms of both security and efficiency.
Optimizing Sample Size for Population Genomic Study in a Global Invasive Lady Beetle, Harmonia Axyridis
Authors: Li Hongran , Qu Wanmei , Obrycki John J. , Meng Ling , Zhou Xuguo , Chu Dong , Li Baoping
Published: May 2020

Finding optimal sample sizes is critical for the accurate estimation of genetic diversity of large invasive populations. Based on previous studies, we hypothesized that a minimal sample size of 3–8 individuals is sufficient to dissect the population architecture of the harlequin lady beetle, Harmonia axyridis, a biological control agent and an invasive alien species. Here, equipped with a type IIB endonuclease restriction site-associated (2b-RAD) DNA sequencing approach, we identified 13,766 and 13,929 single nucleotide polymorphisms (SNPs), respectively, among native and invasive H. axyridis populations. With this information we simulated populations using a randomly selected 3000 SNPs and a subset of individuals. From this simulation we finally determined that six individuals is the minimum sample size required for the accurate estimation of intra- and inter-population genetic diversity within and across H. axyridis populations. Our findings provide an empirical advantage for population genomic studies of H. axyridis in particular and suggest useful tactics for similar studies on multicellular organisms in general.
Life History Evaluation of Ooencyrtus lucidus, a Newly Described Egg Parasitoid of Bagrada hilaris
Authors: Ganjisaffar Fatemeh , Perring Thomas M.
Published: May 2020

Ooencyrtus lucidus Triapitsyn & Ganjisaffar (Hymenoptera: Encyrtidae) was recently recovered from fresh sentinel eggs of Bagrada hilaris (Burmeister) (Hemiptera: Pentatomidae) in California. In the present study, life history traits of O. lucidus were studied at 26 ± 0 . 5 ∘ C, 40 ± 10 % RH, and a photoperiod of 14 L: 10 D hours. Results showed that 95.2% of the parasitized eggs yielded successful emergence of adults. The total immature developmental time was 14.27 and 13.95 days for females and males, respectively. The sex ratio of O. lucidus was 0.5 (females/offspring). Mated females laid on average 6.0 eggs per day and 222.7 eggs during their mean ovipositional period of 37.3 days. There was no significant difference in reproduction parameters between mated and non-mated females. The net reproductive rate ( R 0 ) was 103.8 females/female/generation, the intrinsic rate of increase ( r m ) was 0.171 females/female/day, the finite rate of increase ( λ ) was 1.187 population multiplication/day, the mean generation time (T) was 27.1 days, and the doubling time (DT) was 4.0 days. The highest longevity was observed in mated and non-mated females fed with honey, and they lived for 70.8 and 70.1 days, respectively. Providing females with host eggs in addition to honey, reduced their longevity by 24% in mated and 28% in non-mated individuals. Non-mated females and males deprived of honey only lived for 3.5 and 2.5 days after emergence, respectively. Our results indicate that O. lucidus has sufficient life history traits to make it a promising egg parasitoid for the biological control of B. hilaris.
DNA Metabarcoding as a Tool for Disentangling Food Webs in Agroecosystems
Authors: Sow Ahmadou , Haran Julien , Benoit Laure , Galan Maxime , Brévault Thierry
Published: May 2020

Better knowledge of food webs and related ecological processes is fundamental to understanding the functional role of biodiversity in ecosystems. This is particularly true for pest regulation by natural enemies in agroecosystems. However, it is generally difficult to decipher the impact of predators, as they often leave no direct evidence of their activity. Metabarcoding via high-throughput sequencing (HTS) offers new opportunities for unraveling trophic linkages between generalist predators and their prey, and ultimately identifying key ecological drivers of natural pest regulation. Here, this approach proved effective in deciphering the diet composition of key predatory arthropods (nine species.; 27 prey taxa), insectivorous birds (one species, 13 prey taxa) and bats (one species; 103 prey taxa) sampled in a millet-based agroecosystem in Senegal. Such information makes it possible to identify the diet breadth and preferences of predators (e.g., mainly moths for bats), to design a qualitative trophic network, and to identify patterns of intraguild predation across arthropod predators, insectivorous vertebrates and parasitoids. Appropriateness and limitations of the proposed molecular-based approach for assessing the diet of crop pest predators and trophic linkages are discussed.
Transcriptome Analysis of Gene Expression Profiles of Tomato Yellow Leaf Curl Virus-Infected Whiteflies over Different Viral Acquisition Access Periods
Authors: Li Meng , Zhao Jing , Su Yun-Lin
Published: May 2020

Tomato yellow leaf curl virus (TYLCV), which is transmitted by Bemisia tabaci in a persistent-circulative manner, threatens tomato production worldwide. Little is known about the complicated interaction during this process at the molecular level. In this study, viral AAPs at 0 h, 2 h, 6 h, 12 h and 48 h were investigated using a comparative transcriptome analysis to uncover the transcriptional responses of whiteflies to virus infection. Our results have shown that 755, 587, 1140 and 1347 differentially expressed genes (DEGs) were identified in the comparisons of the data of 0 h vs. 2 h, 0 h vs. 6 h, 0 h vs. 12 h and 0 h vs. 48 h, respectively. KEGG analysis showed that DEGs associated with metabolisms and signal transduction were down-regulated in virus-infected whiteflies. Additionally, 16 up-regulated putative transporter genes and 10 down-regulated genes associated with IL-17 signaling pathway were identified by time-associated gene cluster analysis. These data boost our comprehensions on whitefly-TYLCV interactions associated with different viral AAPs.
Association of Taurine with In-Hospital Mortality in Patients after Out-of-Hospital Cardiac Arrest: Results from the Prospective, Observational COMMUNICATE Study
Authors: Herzog Naemi , Laager Rahel , Thommen Emanuel , Widmer Madlaina , Vincent Alessia M. , Keller Annalena , Becker Christoph , Beck Katharina , Perrig Sebastian , Bernasconi Luca , Neyer Peter , Marsch Stephan , Schuetz Philipp , Sutter Raoul , Tisljar Kai , Hunziker Sabina
Published: May 2020

Background: Studies have suggested that taurine may have neuro- and cardio-protective functions, but there is little research looking at taurine levels in patients after out-of-hospital cardiac arrest (OHCA). Our aim was to evaluate the association of taurine with mortality and neurological deficits in a well-defined cohort of OHCA patients. Methods: We prospectively measured serum taurine concentration in OHCA patients upon admission to the intensive care unit (ICU) of the University Hospital Basel (Switzerland). We analyzed the association of taurine levels and in-hospital mortality (primary endpoint). We further evaluated neurological outcomes assessed by the cerebral performance category scale. We calculated logistic regression analyses and report odds ratios (OR) and 95% confidence intervals (CI). We calculated different predefined multivariable regression models including demographic variables, comorbidities, initial vital signs, initial blood markers and resuscitation measures. We assessed discrimination by means of area under the receiver operating curve (ROC). Results: Of 240 included patients, 130 (54.2%) survived until hospital discharge and 110 (45.8%) had a favorable neurological outcome. Taurine levels were significantly associated with higher in-hospital mortality (adjusted OR 4.12 (95%CI 1.22 to 13.91), p = 0.02). In addition, a significant association between taurine concentration and a poor neurological outcome was observed (adjusted OR of 3.71 (95%CI 1.13 to 12.25), p = 0.03). Area under the curve (AUC) suggested only low discrimination for both endpoints (0.57 and 0.57, respectively). Conclusion: Admission taurine levels are associated with mortality and neurological outcomes in OHCA patients and may help in the risk assessment of this vulnerable population. Further studies are needed to assess whether therapeutic modulation of taurine may improve clinical outcomes after cardiac arrest.
Utility of Flexible Bronchoscopy for Airway Foreign Bodies Removal in Adults
Authors: Sancho-Chust Jose N. , Molina Virginia , Vañes Sandra , Pulido Ana M. , Maestre Lia , Chiner Eusebi
Published: May 2020

Foreign body aspiration is relatively infrequent in adults. Airway foreign bodies (AFBs) can be removed by flexible bronchoscopy (FB) or rigid bronchoscopy (RB). We performed a retrospective analysis of FBs performed in our centre over a 25 year period, focusing on the procedures that revealed an AFB during the examination stage. We recorded demographic data, clinical characteristics and radiological and bronchoscopic findings. During the study period, 12,588 FBs were performed in adults. Airway foreign bodies were identified in 32 of these cases, giving a prevalence of 0.25%. The most frequent clinical presentation was cough, sputum and fever. The most frequent radiological findings were alveolar infiltrates and atelectasis. In 94% of cases, AFBs were removed successfully by FB; RB was necessary in only 6% of cases. There were no FB-related complications. The most common AFB location was the right bronchial tree (69%). We classified AFBs as organic (85%: animal 57%; vegetable 28%), inorganic (6%) and iatrogenic (9%). Bronchial infection occurred in 51% of cases, and Actinomyces spp. was the most common causal microorganism. In conclusion, AFBs are a rare entity with nonspecific clinical presentation, most AFBs were organic, and FB is a safe and effective method for AFB removal.
Lasers in Transurethral Enucleation of the Prostate—Do We Really Need Them
Authors: Herrmann Thomas R.W. , Gravas Stavros , de la Rosette Jean JMCH , Wolters Mathias , Anastasiadis Aristotelis G. , Giannakis Ioannis
Published: May 2020

The acronym EEP, coding for transurethral Endoscopic Enucleation of the Prostate, was introduced in 2016 by the European Association of Urology (EAU) guidelines panel on management of non-neurogenic male lower urinary tract symptoms (LUTS) and benign prostatic obstruction (BPO). Since then, a laser-based treatment, Holmium Laser Enucleation of the Prostate (HoLEP), and the current-based treatment of bipolar enucleation of the prostate (BipoLEP) are equally appreciated as valuable options for the management of benign prostatic obstruction (BPO). This was mainly inspired by the results of two meta-analyses on randomized controlled trials, comparing open prostatectomy with either Holmium Laser Enucleation of the Prostate (HoLEP) or bipolar enucleation of the prostate (BipoLEP). Prior to that, HoLEP was embraced as the only valid option for transurethral enucleation, although evidence for equivalence existed as early as 2006, but was not recognized due to a plethora of acronyms for bipolar energy-based treatments and practiced HoLEP-centrism. On the other hand, the academic discourse focused on different (other) laser approaches that came up, led by Thulium:Yttrium-Aluminum-Garnet (Tm:YAG) Vapoenucleation (ThuVEP) in 2009 and, finally, transurethral anatomical enucleation with Tm:YAG support (thulium laser enucleation of the prostate, ThuLEP) in 2010. Initially, the discourse on lasers focused on the different properties of lasers rather than technique or surgical anatomy, respectively. In and after 2016, the discussion ultimately moved towards surgical technique and accepting anatomical preparation as the common of all EEP techniques (AEEP). Since then, the unspoken question has been raised, whether lasers are still necessary to perform EEP in light of existing evidence, given the total cost of ownership (TCO) for these generators. This article weighs the current evidence and comes to the conclusion that no evidence of superiority of one modality over another exists with regard to any endpoint. Therefore, in the sense of critical importance, AEEP can be safely and effectively performed without laser technologies and without compromise.
Hearing Loss and Risk of Overall, Injury-Related, and Cardiovascular Mortality: The Kangbuk Samsung Health Study
Authors: Lee Woncheol , Chang Yoosoo , Shin Hocheol , Ryu Seungho
Published: May 2020

Hearing loss (HL) has been related to cardiovascular risk factors as well as prevalence of cardiovascular disease itself. We evaluated the association of HL with overall, injury-related, and cardiovascular mortality. A cohort study included 580,798 Korean adults (mean age: 39.7) who attended a screening exam between 2002 and 2016 with a follow-up of up to 17 years. HL was defined as a pure-tone average of thresholds at 0.5, 1.0, and 2.0 kHz ≥25 dB (decibels) in the better ear and further categorized into mild (25–<40 dB) and moderate-to-severe (≥40 dB). Overall and cause-specific mortality was ascertained through linkage to national death records. During median follow-up of 8.4 years, 6581 overall deaths, 977 cardiovascular deaths, and 1161 injury-related deaths were identified. Compared to participants with normal hearing, multivariable-adjusted hazard ratios (HRs) with 95% confidence intervals (95% CIs) for overall mortality among participants with mild and moderate-to-severe HL were 1.13 (1.05–1.21) and 1.30 (1.16–1.46), respectively. Corresponding HRs (95% CIs) for cardiovascular mortality were 1.32 (1.10–1.58) and 1.53 (1.16–2.01), respectively, and corresponding HRs (95% CIs) for injury-related mortality were 1.03 (0.81–1.31) and 1.64 (1.13–2.36), respectively. In this large cohort, HL was positively and independently associated with overall, cardiovascular, and injury-related mortality. A significantly elevated risk of cardiovascular mortality started from mild HL.
Effects of Aerobic Exercise on Cortisol Stress Reactivity in Response to the Trier Social Stress Test in Inpatients with Major Depressive Disorders: A Randomized Controlled Trial
Authors: Gerber Markus , Imboden Christian , Beck Johannes , Brand Serge , Colledge Flora , Eckert Anne , Holsboer-Trachsler Edith , Pühse Uwe , Hatzinger Martin
Published: May 2020

Physical activity is associated with a favourable (blunted) cortisol stress reactivity in healthy people. However, evidence from experimental study and with psychiatric patients is missing. This study examines whether exercise training impacts on cortisol stress reactivity in inpatients with major depressive disorder (MDD). These new insights are important because the stress reactivity of healthy people and patients with severe symptoms of depression might differ. Methods: The study was designed as a randomized controlled trial (trial registration number: NCT02679053). In total, 25 patients (13 women, 12 men, mean age: 38.1 ± 12.0 years) completed a laboratory stressor task before and after a six-week intervention period. Nine samples of salivary free cortisol were taken before and after the Trier social stress test (TSST). Fourteen participants took part in six weeks of aerobic exercise training, while 11 patients were allocated to the control condition. While the primary outcome of the study was depressive symptom severity, the focus of this paper is on one of the secondary outcomes (cortisol reactivity during the TSST). The impact of aerobic exercise training was examined with a repeated-measures analysis of variance. We also examined the association between change in depression and cortisol response via correlational analysis. Cortisol reactivity did not change from baseline to post-intervention, either in the intervention or the control group. Participation in six weeks of aerobic exercise training was not associated with participants’ cortisol reactivity. Moreover, depressive symptom change was not associated with change in cortisol response. Aerobic exercise training was not associated with patients’ stress reactivity in this study. Because many patients initially showed a relatively flat/blunted cortisol response curve, efforts might be needed to find out which treatments are most efficient to promote a normalization of HPA axis reactivity.
Association between Very Low-Density Lipoprotein Cholesterol (VLDL-C) and Carotid Intima-Media Thickness in Postmenopausal Women Without Overt Cardiovascular Disease and on LDL-C Target Levels
Authors: Gentile Marco , Iannuzzi Arcangelo , Giallauria Francesco , D’Andrea Antonello , Venturini Elio , Pacileo Mario , Covetti Giuseppe , Panico Camilla , Mattiello Amalia , Vitale Giuseppe , Sarullo Filippo Maria , Rubba Paolo , Vigorito Carlo , Panico Salvatore , Iannuzzo Gabriella
Published: May 2020

Background: atherosclerotic process inexorably advances in patients reaching low-density lipoprotein cholesterol (LDL-C) targets. An attractive hypothesis is that lipoprotein particles (very low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL)), could contribute to residual risk. The present study aims to investigate the relationship between carotid intima-media thickness (IMT) and different lipoprotein subfractions in a cohort of healthy postmenopausal women. Methods: 75 postmenopausal women, at LDL-C target levels without overt cardiovascular disease, underwent biochemical analyses (including subfraction assay of plasma lipoproteins) and carotid ultrasound examination. Results: a statistically significant correlation between VLDL and carotid IMT (p < 0.001) was found. No significant correlation was found between carotid IMT and LDL-C (p = 0.179), IDL-C (p = 0.815), high-density lipoprotein (HDL) (p = 0.855), and LDL score (p = 0.240). Moreover, IMT is significantly correlated to LDL particle diameter (p = 0.044). After adjusting for age, systolic blood pressure, body mass index, smoking habits, glucose plasma concentration, and Lipoprotein(a) (Lpa) levels, multivariate analysis showed that women in the third tertile of VLDL-C, compared with those in the first tertile, were significantly associated to the highest IMT (p = 0.04). Conclusions: in this cohort of postmenopausal women, VLDL-C was significantly associated to carotid IMT, independent of main cardiovascular risk factors. These findings pave the way for targeting circulating concentrations of VLDL-C to reduce cardiovascular events in patients with target LDL-C levels.
Bleeding Severity in Percutaneous Coronary Intervention (PCI) and Its Impact on Short-Term Clinical Outcomes
Authors: Murali Shashank , Vogrin Sara , Noaman Samer , Dinh Diem T. , Brennan Angela L. , Lefkovits Jeffrey , Reid Christopher M. , Cox Nicholas , Chan William
Published: May 2020

Bleeding severity in patients undergoing percutaneous coronary intervention (PCI), defined by the Bleeding Academic Research Consortium (BARC), portends adverse prognosis. We analysed data from 37,866 Australian patients undergoing PCI enrolled in the Victorian Cardiac Outcomes Registry (VCOR), and investigated the association between increasing BARC severity and in-hospital and 30-day major adverse cardiac and cerebrovascular events (MACCE) (a composite of mortality, myocardial infarction, stent thrombosis, target vessel revascularisation, or stroke). Independent predictors associated with major bleeding (BARC groups 3&5), and MACCE were also assessed. There was a stepwise increase in in-hospital and 30-day MACCE with greater severity of bleeding. Independent predictors of bleeding included female sex (Odds Ratio (OR) 1.34), age (OR 1.02), fibrinolytic therapy (OR 1.77), femoral access (OR 1.51), and ticagrelor (OR 1.42), all significant at the p < 0.001 level. Following adjustment of clinically important variables, BARC 3&5 bleeds (OR 4.37) were still predictive of cumulative in-hospital and 30-day MACCE. In conclusion, major bleeding is an uncommon but potentially fatal PCI complication and was independently associated with greater MACCE rates. Efforts to mitigate the occurrence of bleeding, including radial access and judicious use of potent antiplatelet therapies, may ameliorate the risk of short-term adverse clinical outcomes.
MSI and EBV Positive Gastric Cancer’s Subgroups and Their Link with Novel Immunotherapy
Authors: Rodriquenz Maria Grazia , Roviello Giandomenico , D’Angelo Alberto , Lavacchi Daniele , Roviello Franco , Polom Karol
Published: May 2020

Gastric cancers have been historically classified based on histomorphologic features. The Cancer Genome Atlas network reported the comprehensive identification of genetic alterations associated with gastric cancer, identifying four distinct subtypes— Epstein-Barr virus (EBV)-positive, microsatellite-unstable/instability (MSI), genomically stable and chromosomal instability. In particular, EBV-positive and MSI gastric cancers seem responsive to novel immunotherapies drugs. The aim of this review is to describe MSI and EBV positive gastric cancer’s subgroups and their relationship with novel immunotherapy.
25-Hydroxyvitamin D, Vitamin D Binding Protein, Bioavailable 25-Hydroxyvitamin D, and Body Composition in a Diverse Sample of Women Collegiate Indoor Athletes
Authors: Fields Jennifer B. , Gallo Sina , Worswick Jenna M. , Busteed Deanna R. , Jones Margaret T.
Published: May 2020

Women athletes are at higher risk for bone diseases; yet, information on vitamin D status ((25(OH)D), vitamin D binding protein (VDBP), and bioavailable 25(OH)D is limited. Collegiate athletes (n = 36) from volleyball (WVB), basketball (WBB), and track and field (WTF) were measured for (25(OH)D), VDBP, and bioavailable 25(OH)D; body composition and bone mineral density (BMD); and skin pigmentation. Participants self-reported daily vitamin D intake and sun exposure. One-way analysis of variance analyzed mean differences in measures across sports. Linear regression examined relationships between 25(OH)D; VDBP; bioavailable 25(OH)D; and whole body, hip, and spine BMD. Participants’ (mean ± SD, 19.4 ± 1.4 years, 172.75 ± 8.21 cm, 70.9 ± 13.2 kg, and 22.9 ± 4.1% body fat) overall mean 25(OH)D was 70.5 ± 32.25 nmol/L, and 28% of participants were deemed inadequate and 61% below thresholds identified as sufficient for athletes. Although WBB athletes consumed higher (p = 0.007) dietary vitamin D (760.9 ± 484.2 IU/d) than WVB (342.6 ± 257.8) and WTF (402.3 ± 376.4) athletes did, there were no differences across sport in serum 25(OH)D. WVB and WTF had higher bioavailable 25(OH)D than WBB. No relationships existed between vitamin D status and body composition. Vitamin D inadequacy was identified among 1/3 of women indoor sport athletes. Consistent monitoring of vitamin D status and diet are recommended to sustain athlete health and sport performance.
Developing a Pilot Operational Oceanography System for an Enclosed Basin
Authors: Petalas Stamatios , Mamoutos Ioannis , Dimitrakopoulos Agisilaos-Alexandros , Sampatakaki Angeliki , Zervakis Vassilis
Published: May 2020

The first stages of the development of a pilot oceanographic observatory for an enclosed basin (Kalloni Gulf, Lesvos, Greece) are described. The focus of the present work is related to the estimation of the water exchange with the open sea, using a numerical model of the region, volume flux measurements based on current velocity, and sea-level measurements. Three different methods of assessment of the exchange are described and evaluated, in order to select a reference method to calibrate submarine telephone cable measurements. The high-resolution coastal circulation model for the enclosed sea, nested in a larger-domain model, is developed and evaluated against in situ data, focusing on the adequate representation of the exchange and the hydrographic structure in the basin. Monitoring the water level is selected as the best-suited method for estimating the water exchange in hourly-to-weekly time scales. The model reproduces adequately the tidal exchange and sea-level response, as well as the hydrographic characteristics of the basin. The cable voltage measurements exhibit tidal signals overwhelmed by low-frequency noise, possibly attributable to circuitry and ground failures; however, the most suitable method for estimating exchanges of the basin with the open sea appears to be sea-level monitoring.
Surface Crack Growth in Offshore Metallic Pipes under Cyclic Loads: A Literature Review
Authors: Li Zongchen , Jiang Xiaoli , Hopman Hans
Published: May 2020

The surface crack, also known as the partly through-thickness crack, is a serious threat to the structural integrity of offshore metallic pipes. In this paper, we review the research progress in regard to surface crack growth in metallic pipes subjected to cyclic loads from the fracture mechanics perspective. The purpose is to provide state-of-the-art investigations, as well as indicate the remaining challenges. First, the available studies on surface cracked metallic pipes are overviewed from experimental, numerical, and analytical perspectives, respectively. Then, we analyse state-of-the-art research and discuss the insufficiencies of the available literature from different perspectives, such as surface cracks and pipe configurations, environmental influential parameters, the girth welding effect, and numerical and analytical evaluation methods. Building on these surveys and discussions, we identify various remaining challenges and possible further research topics that are anticipated to be of significant value both for academics and practitioners.
Flume Experiment on the Sediment-Retaining Effect of Submerged Breakwaters under the Combined Action of Waves and Currents
Authors: Yan Bing , Zhang Ruibo , Zhang Puyang
Published: May 2020

This research was initiated as design guidance for the extension works of a submerged trapezoidal riprap breakwater protecting the channel towards Huanghua Port in China. Based on flume experiments, the sediment-retaining effect of the breakwater was investigated in two sections under the combined action of currents and waves. We also discussed differences due to two kinds of facing layer—Accropodes and mold bag concrete. Three main observations are provided herein. First, the suspended sediments in front of breakwaters are mainly concentrated in the near-bottom 30% water depth scope and the interface can be seen clearly between the high concentration suspension and the upper clear water. Second, the sediment-retaining ratio of breakwater at −5.7 m isobath position is about 37%–49% and that at −7.3 m isobath position is about 61%–65%. Last, when there are wave breakings in the vicinity of submerged breakwater crest, the facing layers cause some differences of sediment-retaining performances.
Integrated Management of Aphis craccivora in Cowpea Using Intercropping and Entomopathogenic Fungi under Field Conditions
Authors: Mweke Allan , Akutse Komivi Senyo , Ulrichs Christian , Fiaboe Komi Kouma Mokpokpo , Maniania Nguya Kalemba , Ekesi Sunday
Published: May 2020

Cowpea aphid, Aphis craccivora, is a major cowpea pest. Cowpea–cereal intercrop alone does not effectively manage the pest. Use of pesticides in intercrop leads to health and environmental risks. Fungal-based biopesticides offer a better option because they are environment- and consumer-friendly. This study assessed the combined effect of Metarhizium anisopliae ICIPE 62 and cowpea–maize intercrop against A. craccivora under six treatments: (1) untreated cowpea monocrop, (2) untreated cowpea–maize intercrop, (3) cowpea monocrop + ICIPE 62, (4) cowpea–maize intercrop + ICIPE 62, (5) cowpea monocrop + Duduthrin insecticide, and (6) cowpea–maize intercrop + Duduthrin during three seasons (long rainy/cold and dry/short rainy). In the cold and dry season, cowpea–maize intercrop treated with ICIPE 62 recorded the lowest infestation/cowpea damage, whereas the leaf yield was comparable to cowpea monocrop treated with ICIPE 62. In the short rainy season, the cowpea–maize intercrop treated with ICIPE 62 recorded the lowest infestation/damage, whereas leaf yield was similar to cowpea–maize intercrop treated with ICIPE 62 in the cold and dry season. Duduthrin in monocrop and intercrop did not reduce aphid infestation/cowpea damage levels in all the seasons. Although the efficacy of M. anisopliae ICIPE 62-based biopesticide could be affected by seasons, it successfully controlled aphid population in cowpea–maize intercrop under field conditions without affecting aphid-associated natural enemies.
Future Trends in Nebulized Therapies for Pulmonary Disease
Authors: McCarthy Sean D. , González Héctor E. , Higgins Brendan D.
Published: May 2020

Aerosol therapy is a key modality for drug delivery to the lungs of respiratory disease patients. Aerosol therapy improves therapeutic effects by directly targeting diseased lung regions for rapid onset of action, requiring smaller doses than oral or intravenous delivery and minimizing systemic side effects. In order to optimize treatment of critically ill patients, the efficacy of aerosol therapy depends on lung morphology, breathing patterns, aerosol droplet characteristics, disease, mechanical ventilation, pharmacokinetics, and the pharmacodynamics of cell-drug interactions. While aerosol characteristics are influenced by drug formulations and device mechanisms, most other factors are reliant on individual patient variables. This has led to increased efforts towards more personalized therapeutic approaches to optimize pulmonary drug delivery and improve selection of effective drug types for individual patients. Vibrating mesh nebulizers (VMN) are the dominant device in clinical trials involving mechanical ventilation and emerging drugs. In this review, we consider the use of VMN during mechanical ventilation in intensive care units. We aim to link VMN fundamentals to applications in mechanically ventilated patients and look to the future use of VMN in emerging personalized therapeutic drugs.
Agricultural Landscape Composition Linked with Acoustic Measures of Avian Diversity
Authors: Dixon Adam P. , Baker Matthew E. , Ellis Erle C.
Published: May 2020

Measuring, monitoring, and managing biodiversity across agricultural regions depends on methods that can combine high-resolution mapping of landscape patterns with local biodiversity observations. This study explores the potential to monitor biodiversity in agricultural landscapes by linking high-resolution remote sensing with passive acoustic monitoring. Land cover maps produced using a small unmanned aerial system (UAS) and PlanetScope (PS) satellite imagery were used to investigate relationships between landscape patterns and an acoustically derived biodiversity index (vocalizing bird species richness) across 12 agricultural sample locations equipped with acoustic recorders in Iowa, USA during the 2018 growing season. Statistical assessment revealed a significant direct association between vocalizing bird richness and percent noncrop vegetation cover. High spatial resolution (1 m) UAS mapping produced stronger statistical associations than PS-based maps (3 m) for landscape composition metrics. Landscape configuration metrics (Shannon’s diversity index, contagion, perimeter-area-ratio, and circumscribing circle index) were either cross-correlated with composition metrics or unusable owing to complete landscape homogeneity in some agricultural landscape samples. This study shows that high resolution mapping of noncrop vegetation cover can be linked with acoustic monitoring of unique bird vocalizations to provide a useful indicator of biodiversity in agricultural landscapes.
Key Roles for Landscape Ecology in Transformative Agriculture Using Aotearoa—New Zealand as a Case Example
Authors: Pearson Diane
Published: May 2020

Aotearoa—New Zealand (NZ) is internationally renowned for picturesque landscapes and agricultural products. Agricultural intensification has been economically beneficial to NZ but has implications for its clean green image. Contaminated waterways, high carbon emissions, and extensive soil erosion demonstrate the downside of high stocking rates and land clearing. Transformative farming systems are required to address the challenge of balancing production with the environment. Whilst navigating through the process of change, farmers need to be supported to make informed decisions at the farm and landscape scale. Landscape ecology (LE) is ideally positioned to inform the development of future farming landscapes and provide a scientific context to the criteria against which land-related information can be evaluated. However, to do this effectively, LE needs to demonstrate that it can link theory with practice. Using NZ as a case example, this paper discusses the key roles for LE in future farming systems. It looks at the way LE can help quantify the state of the landscape, provide support towards the co-creation of alternative futures, and assist with the inclusion of land-related information into design and planning to ensure mitigation and adaption responses assist in the transformation of farming systems for sustainable outcomes.
High-Throughput Identification of MiR-145 Targets in Human Articular Chondrocytes
Authors: Martinez-Sanchez Aida , Lazzarano Stefano , Sharma Eshita , Lockstone Helen , Murphy Christopher L.
Published: May 2020

MicroRNAs (miRNAs) play key roles in cartilage development and homeostasis and are dysregulated in osteoarthritis. MiR-145 modulation induces profound changes in the human articular chondrocyte (HAC) phenotype, partially through direct repression of SOX9. Since miRNAs can simultaneously silence multiple targets, we aimed to identify the whole targetome of miR-145 in HACs, critical if miR-145 is to be considered a target for cartilage repair. We performed RIP-seq (RNA-immunoprecipitation and high-throughput sequencing) of miRISC (miRNA-induced silencing complex) in HACs overexpressing miR-145 to identify miR-145 direct targets and used cWords to assess enrichment of miR-145 seed matches in the identified targets. Further validations were performed by RT-qPCR, Western immunoblot, and luciferase assays. MiR-145 affects the expression of over 350 genes and directly targets more than 50 mRNAs through the 3′UTR or, more commonly, the coding region. MiR-145 targets DUSP6, involved in cartilage organization and development, at the translational level. DUSP6 depletion leads to MMP13 upregulation, suggesting a contribution towards the effect of miR-145 on MMP13 expression. In conclusion, miR-145 directly targets several genes involved in the expression of the extracellular matrix and inflammation in primary chondrocytes. Thus, we propose miR-145 as an important regulator of chondrocyte function and a new target for cartilage repair.
A Time-Frequency Based Approach for Acoustic Emission Assessment of Sliding Wear
Authors: Rastegaev Igor , Merson Dmitry , Rastegaeva Inna , Vinogradov Alexei
Published: May 2020

The acoustic emission method is one of few contemporary non-destructive testing techniques enabling continuous on-line health monitoring and control of tribological systems. However, the existence of multiple “pseudo”-acoustic emission (AE) and noise sources during friction, and their random occurrence poses serious challenges for researchers and practitioners when extracting “useful” information from the upcoming AE signal. These challenges and numerous uncertainties in signal classification prevent the unequivocal interpretation of results and hinder wider uptake of the AE technique despite its apparent advantages. Currently, the signal recording and processing technologies are booming, and new applications are born on this support. Specific tribology applications, therefore, call for developing new and tuning existing approaches to the online AE monitoring and analysis. In the present work, we critically analyze, compare and summarize the results of the application of several filtering techniques and AE signal classifiers in model tribological sliding friction systems allowing for the simulation of predominant wear mechanisms. Several effective schemes of AE data processing were identified through extensive comparative studies. Guidelines were provided for practical application, including the online monitoring and control of the systems with friction, characterizing the severity and timing of damage, on-line evaluation of wear as sliding contact tests and instrumented acceleration of tribological testing and cost reduction.
Effect of Pre-Shear on Agglomeration and Rheological Parameters of Cement Paste
Authors: Thiedeitz Mareike , Dressler Inka , Kränkel Thomas , Gehlen Christoph , Lowke Dirk
Published: May 2020

Cementitious pastes are multiphase suspensions that are rheologically characterized by viscosity and yield stress. They tend to flocculate during rest due to attractive interparticle forces, and desagglomerate when shear is induced. The shear history, e.g., mixing energy and time, determines the apparent state of flocculation and accordingly the particle size distribution of the cement in the suspension, which itself affects suspension’s plastic viscosity and yield stress. Thus, it is crucial to understand the effect of the mixing procedure of cementitious suspensions before starting rheological measurements. However, the measurement of the in-situ particle agglomeration status is difficult, due to rapidly changing particle network structuration. The focused beam reflectance measurement (FBRM) technique offers an opportunity for the in-situ investigation of the chord length distribution. This enables to detect the state of flocculation of the particles during shear. Cementitious pastes differing in their solid fraction and superplasticizer content were analyzed after various pre-shear histories, i.e., mixing times. Yield stress and viscosity were measured in a parallel-plate-rheometer and related to in-situ measurements of the chord length distribution with the FBRM-probe to characterize the agglomeration status. With increasing mixing time agglomerates were increasingly broken up in dependence of pre-shear: After 300 s of pre-shear the agglomerate sizes decreased by 10 µm to 15 µm compared to a 30 s pre-shear. At the same time dynamic yield stress and viscosity decreased up to 30% until a state of equilibrium was almost reached. The investigations show a correlation between mean chord length and the corresponding rheological parameters affected by the duration of pre-shear.
Surface Comparison of Three Different Commercial Custom-Made Titanium Meshes Produced by SLM for Dental Applications
Authors: Cruz Nuno , Martins Maria Inês , Domingos Santos José , Gil Mur Javier , Tondela João Paulo
Published: May 2020

The use of individualized titanium meshes has been referred to in scientific literature since 2011. There are many advantages to its use, however, the main complications are related to early or late exposures. As some aspects such as its surface properties have been pointed out to influence the soft tissue response, this study was designed to compare the surface characteristics of three commercially available individualized titanium meshes between them and according to the manufacturer’s specifications. The results from the scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction and the contact profilometry measurements were analyzed and cross-checked. It was discovered that, the BoneEasy’s post-processing superficial treatment was more refined, as it delivers the mesh with the lowest Ra value, 0.61 ± 0.14 µm, due to the applied electropolishing. On the other hand, the Yxoss CBR® mesh from ReOss® was sandblasted, presenting an extremely rough surface with a Ra of 6.59 ± 0.76 µm.
On the Chip Shaping and Surface Topography When Finish Cutting 17-4 PH Precipitation-Hardening Stainless Steel under Near-Dry Cutting Conditions
Authors: Leksycki Kamil , Feldshtein Eugene , Królczyk Grzegorz M. , Legutko Stanisław
Published: May 2020

This study describes the surface topography of the 17-4 PH stainless steel machined under dry, wet and near-dry cutting conditions. Cutting speeds of 150–500 m/min, feeds of 0.05–0.4 mm/rev and 0.5 mm depth of cutting were applied. The research was based on the ‘parameter space investigation’ method. Surface roughness parameters, contour maps and material participation curves were analysed using the optical Sensofar S Neox 3D profilometer and the effect of feed, cutting speed and their mutual interaction was noticed. Changes in chip shape depending on the processing conditions are shown. Compared to dry machining, a reduction of Sa, Sq and Sz parameters of 38–48% was achieved for near-dry condition. For lower feeds and average cutting speeds valleys and ridges were observed on the surface machined under dry, wet and near-dry conditions. For higher feeds and middle and higher cutting speeds, deep valleys and high ridges were observed on the surface. Depending on the processing conditions, different textures of the machined surface were registered, particularly anisotropic mixed, periodic and periodically determined. In the Sa range of 0.4–0.8 μm for dry and wet conditions the surface isotropy is ~20%, under near-dry conditions it is ~60%.