Study of Clinical, Hematologic and Immunologic Parameters in Assessing the Anti-Radiation Efficacy of the Therapeutic Agent Based on the Microorganism Fusobacterium Necrophorum
Authors:   Federal Center for Toxicological, Radiation and Biological Safety, Kazan, Russia, Gaynutdinov T.R. ,   Kazan Federal University, Kazan, Russia, Ryzhkin S.A. ,   Federal Center for Toxicological, Radiation and Biological Safety, Kazan, Russia,   Kazan Federal University, Kazan, Russia,   Russian Medical Academy of Continuing Professional Education, Moscow, Russia,   Kazan State Medical University, Kazan,   Academy of Sciences of the Republic of Tatarstan, Kazan, Russia, Vagin K.N. ,   Federal Center for Toxicological, Radiation and Biological Safety, Kazan, Russia,   Kazan Federal University, Kazan, Russia, Trizna E.Yu. ,   Kazan Federal University, Kazan, Russia, Ohrimenko S.E. ,   Russian Medical Academy of Continuing Professional Education, Moscow, Russia,   A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Published: June 2024

Purpose: To study clinical, hematologic and immunologic parameters in assessing the anti-radiation efficacy of the therapeutic agent based on the microorganism Fusobacterium necrophorum. Material and methods: The studies on determination of the anti-radiation efficacy of the strains of microorganisms killed by gamma-irradiation were carried out on sexually mature sexless white mice and white rats with live weight of 18–20 and 180–200 g, respectively, divided into experimental and control groups according to the principle of analogs. Modeling of acute radiation sickness was carried out on a Puma gamma unit with a radioactive source of cesium-137 at a dose of LD100/30. As potential anti-radiation drugs we used inactivated by irradiation on the gamma unit Explorer preparations of microbial origin F. necrophorum strain 8. necrophorum strain 8TS630501 at doses of 15, 20, 25 and 30 kGy. The tested preparations were injected subcutaneously in the volume of 0.2 cm3 to white mice and 2.0 cm3 to white rats 3 days after radiation exposure. Results: It was experimentally established that complete sterilization of the microbe occurs at doses of 25 and 30 kGy. The culture of F. necrophorum, irradiated at doses of 25 and 30 kGy and administered to animals 3 days after external radiation exposure, promoted survival, preservation of 60 to 80 % of lethally irradiated white mice and rats. At the same time the recovery of leukocytes and hemoglobin number was slow and continued until the end of the study. In animals treated with the developed therapeutic agents, there was also a decrease in the number of T-cells, but it was less pronounced than in the irradiation control group. The number of B-lymphocytes was affected similarly to T-lymphocytes. The minimum of the number of B-lymphocytes in the experimental groups was noted at 14 days. Studies on the intensity of the process of lipid peroxidation (LPO) in the content of malonic dialdehyde in peripheral blood of gamma-irradiated, treated and intact rats, it was found that in the irradiated control group there is a significant increase in the LPO index in blood in relation to biological control and treatment groups. Conclusion: It has been established that the highest anti-radiation efficacy is possessed by a therapeutic agent of microbial origin (RNF-30), which was obtained by gamma-irradiation at a dose of 30 kGy of the culture of F. necrophorum.