bluuebunny/crossref_metadata_2025 huggingface dataset:
Experimental Analysis of Process Parameter Effects on Vibrations in the High-Speed Grinding of a Camshaft
Authors: Liu Tao , Deng Zhaohui , Lv Lishu , She Shuailong , Liu Wei , Luo Chengyao
Published: March 2020

The capacity to minimize vibrations in grinding by the selection of appropriate process parameters is a significant benefit in the process optimization of cam grinding. This paper presents survey methods to characterize and quantify the vibrations and waviness in a camshaft grinding application. First, a modal analysis was conducted to study the dynamic characteristic of a camshaft grinding machine. Then, developed methods were applied to study the influences of various parameters on vibrations in the high-speed grinding of a camshaft. Furthermore, the influence of each grinding process condition on surface waviness in high-speed camshaft grinding was studied. The results show that the vibrations and the surface waviness change with the increase of grinding depth, and an appropriate grinding wheel speed combined with a workpiece speed has, for most grinding conditions, a reducing effect on vibration magnitudes and waviness. Finally, the specific indications about the optimal grinding process parameters in terms of dynamic characteristics of the grinding machine were given, and the speed ratio=1.2 is a novel choice.
Power-Following Control Strategy of a Wheel-Drive Hydraulic Hybrid Vehicle
Authors: Zhang Tao , Wang Qiang , He Xiao-Hui , Li Si-Sheng , Shen Xin-Min
Published: March 2020

Energy management strategy is a critical technology for improving the fuel economy of wheel-drive hydraulic hybrid vehicles. For driving, a power-following control strategy is proposed in this study by adding several working points of the engine in the optimal fuel economy power curve. For braking, the “I” curve distribution strategy based on critical braking strength zmin was adopted. A test bench was constructed according to the quarter of the prototype vehicle. Taking the typical working conditions of Federal Urban Driving Schedule (FUDS) and the selfset extra-urban driving schedule (EUDC-1) cycle condition into consideration, the energy management strategy was studied. The torque and speed of the simulated engine and pressure of the accumulator were obtained. The test fuel consumption in this research was compared with the original fuel consumption of the prototype vehicle. It was found that the proposed energy management strategy could effectively improve the fuel economy by more than 24 % under the requirement of satisfying the dynamic performance of the whole vehicle.
Die Pflicht zum Schulbesuch
Authors: Hauk Julia
Published: March 2020

The author discusses the constitutional conformity of compulsory schooling and the question of whether parents or pupils have a right to homeschooling – i.e. a right to replace school attendance with other education at home. In her opinion, Article 7 of the German constitution assumes a general obligation to attend school, which results from the regulations on religious education and freedom of private schooling, as well as from the history of the norm. The factual legitimacy of compulsory schooling lies in its democratisation and integration function. The fundamental rights of parents and children do not require the admission of homeschooling either. As fundamental rights affected by compulsory schooling, the author cites the parental right of education and, on the part of the pupils, the general freedom of action, the general right of personality and the freedom of the person. Furthermore, the author deals with the protection of fundamental rights in cases of religiously motivated homeschooling.
Die Ordnungsfunktion des Tarifvertrags
Authors: Holler Daniel Eginhard
Published: March 2020

Die Ordnungsfunktion des Tarifvertrags gehört zu den schillerndsten Begriffen des Tarifvertragsrechts. Sie wird oft und gerne bemüht, um die Erstreckung der Regelungswirkung des Tarifvertrags über den mitgliedschaftlichen Bereich hinaus zu begründen. Aber auch nach ihrer gesetzlichen Normierung in § 1 AEntG und § 4a Abs. 1 TVG bleibt diese Tarifvertragsfunktion umstritten und in ihrem rechtlichen Gehalt unklar.

Daniel Holler nimmt sich daher der Frage nach dieser tarifvertraglichen Ordnungsfunktion an. In seiner rechtsdogmatischen Untersuchung wird die Funktion rechtlich analysiert, strukturiert und in das bestehende Privatrechtssystem eingefügt. Er weist nicht nur nach, dass die Ordnungsfunktion des Tarifvertrags stark limitiert ist, sondern stellt zugleich Beziehungen zu aktuellen praktischen Rechtsproblemen her.
Die Renaissance der Staatenbeschwerde
Authors: Küchler Helen
Published: March 2020

Conflicts in Eastern Ukraine, on the Crimean Peninsula, and in the Balkans and the Caucasus – the Council of Europe system is in crisis, which has led to the renaissance of a long-forgotten instrument of European human rights protection: the interstate complaint procedure under art. 33 of the ECHR. It allows any contracting state as a ‘collective guarantor of the Convention’ (preamble) to refer any alleged breach of the ECHR to its court. After decades of indifference towards human rights violations in other European states, art. 33 of the ECHR became more commonly used after the turn of the millennium. However, it has primarily been misused as a dispute resolution mechanism, instead of its potential being exploited to solve systemic problems concerning the realisation of human rights. Therefore, this study calls for the renaissance of the interstate complaint procedure to be reframed in order for it to contribute to one of humankind’s goals: the effective enforcement of human rights around the globe.
Manzuma gen. nov., a new aelurilline genus of jumping spiders (Araneae, Salticidae)
Authors: Azarkina Galina N.
Published: March 2020

A new jumping spider genus, Manzuma gen. nov. (Salticidae Blackwall, 1841), is described, type species is Manzuma nigritibia (Caporiacco, 1941). Aelurillus reconditus Wesołowska & van Harten, 1994 is synonymized with Rafalus nigritibiis (Caporiacco, 1941). Four new combinations are proposed: M. jocquei gen. et comb. nov. (ex Aelurillus), M. kenyaensis gen. et comb. nov. (ex Langelurillus), M. lympha gen. et comb. nov. (ex Rafalus) and M. nigritibia gen. et comb. nov. (ex Rafalus). Three species, M. botswana gen. et sp. nov. (♂♀, Botswana and Republic of South Africa), M. petroae gen. et sp. nov. (♂♀, Republic of South Africa) and M. tanzanica gen. et sp. nov. (♂, Tanzania), are described. The male of M. kenyaensis gen. et comb. nov. and female of M. lympha gen. et comb. nov. are described for the first time. A new aelurilline synapomorphy is proposed. Identification key for males is provided.
Caracterização das viagens para a escola em cidades de pequeno porte: estudo de caso da região do Vale do Jacuí/RS (Brasil)
Authors: Silva Raquel Cristina Ferreira , Oestreich Letícia , Pereira Brenda Medeiros , Padillo Alejandro Ruiz -
Published: March 2020

School trips characterize the main daily commute by students. Understanding how these trips are made and which aspects influence their choices is fundamental to provide improvements in transportation and quality of life for students. From a literature review, we listed aspects impacting the selection of the transportation mode used in school trips and the satisfaction with the quality of scholar transportation provided to the students. To qualify scholar trips in small cities, high school students from 10 schools located in 6 different cities of the central region of Rio Grande do Sul State were selected, totaling 763 students interviewed. Through the application of a qualitative and quantitative questionnaire we collected data on target audience, trips, perceptions of trips and satisfaction with scholar transportation, providing information for the diagnosis of the scholar trips. With the results obtained, it is possible to guide public policies aimed at improving the quality and safety of scholar trips.
Planejamento urbano e regional: diagnósticos do município de Candelária (RS) e proposta de pista multiuso
Authors: Wollmann Luiza , Speth Gianne , Peres Leonardo Ernandes da Silva , Joris Nadiane Dias , Domingues Quétilan Rodrigues , Porto Rhaíssa Mix , Ribeiro Bárbara Maria Giaccom
Published: March 2020

With the purpose of consolidating, in a strategic way, to encourage and to support the local commerce, sports and cultural aspects in the city of Candelária (Rio Grande do Sul, Brazil), we propose a study of equipment needs that offer entertainment and leisure to the population, preventing residents from moving to other nearby cities to meet those needs. Favored by the flat topography of the site, the solutions interconnect with the proposal to create a multipurpose track, which would encourage the use of the bicycle as a means of transport, not only for working purposes, but also for leisure, as well as providing benefits of greater road safety and travel speed. For this, the proposed urban equipment and its connections with the multipurpose track are analyzed using geoprocessing and virtual reality features. Virtual maps and three-dimensional models help on exemplification of the proposals, enabling better study of the city’s existing potentialities as conditioning factors of the project.
Promoção da mobilidade sustentável através de atividades lúdicas com bicicletas na educação infantil
Authors: Macedo Juliana Aguiar , Pereira Brenda Medeiros , Padillo Alejandro Ruiz-
Published: March 2020

Children connect with the city mainly through their trips to school. Since childhood children can develop the interest in taking care of the public spaces that surrounds them and develop a questioning character. The Pedala Kids extension project seeks to encourage children to ride bikes and interact with urban space through activities aimed at increasing road safety, encouraging active mobility and respect for life, with long-term effects, contributing to a well-functioning of the city. In partnership with a school in Cachoeira do Sul, the Federal University of Santa Maria - Campus Cachoeira do Sul held the Cycling Games. The aim of this paper is to present the experience of the Games as a playful activity that seeks to contribute to citizenship enhancement of children. With four main stages, the Games had four main steps: brief literature review, meetings with partner agencies for project structuring, preparation of supporting material and realization of the Cycling Games’ activities. Thus, this project stimulates the engagement of the academic community in promoting the quality of life of the population of Cachoeira do Sul.
Simulação de uma Unidade de Medição Fasorial em Tempo Real utilizando Typhoon Virtual HIL
Authors: Reis Amanda Wohlfahrt , Guarda Fernando Guilherme Kaehler
Published: March 2020

This paper aims to present a project to implement a phasor measurement unit (PMU), which is the main component in the synchronized phasor measurement system. This measurement technology aims to bring significant gains to the operation of electrical systems, since it allows to simultaneously measure magnitudes and phase angles of voltage and current at geographically distant points from the electrical system. So, the paper reports the use of filters in obtaining PMU measurements. In this way, it is intended to implement the PMU in the Typhoon Virtual HIL software.
Montagem, testes operacionais e validação de uma unidade laboratorial para extração de compostos de matrizes vegetais utilizando fluidos pressurizados ou supercríticos como solventes
Authors: Ferreira Renan De Oliveira , Cunha Estêvão Santos Laureano da , Bitencourte Iuri Prass , Silva Jefferson Fagundes da , Morais Jéssica Rocha de , Silveira Norton Marozo , Teixeira Angelico Loreto , Tres Marcus Vinícius , Zabot Giovani Leone
Published: March 2020

Extractions of compounds still use traditional methods, leaving solvent residues in the extracts. An alternative is the use of high-pressure solvents such as supercritical CO2 and pressurized liquefied petroleum gas (LPG). Based on this context, the objective of this work was to assemble an equipment for high-pressure extractions using CO2 or pressurized LPG as solvent. The project and assembling were divided into 3 steps. In the first step, the equipment was designed. Also, aluminum bars, pipes, valves, pressure gauges, thermostatic baths, a pump for solvent pressurization, a digital temperature controller, a band heater and a reactor with filters were purchased. In the second step, some adaptations were done in the structure and its components for improving the operation. In the third step, the equipment was effectively assembled. Thereafter, operational validation tests were performed in order to verify the operation and the necessity of adjustments. In addition, a Standard Operating Procedure was created in order to standardize the next operations. The equipment is suitable for the extraction of bioactive compounds from different plant matrices, providing satisfactory extraction yields.
Qualidade física de grãos beneficiados de cultivares de arroz produzidos em diferentes níveis de adubação
Authors: Müller Amanda , Coradi Paulo Carteri , Steinhaus Jonatas Ibagé , Souza Guilherme Abreu Coelho de , Carneiro Letícia Oliveira , Jaques Lanes Beatriz Acosta , Grohs Mara
Published: March 2020

Rice is one of the most cultivated cereals in the world and the increase in crop yield is associated with cultivars and levels of soil fertilization, and may suffer changes in grain quality after processing. The objective of this work was to evaluate the physical quality of grains of rice benefited as polished and integral of cultivars IR 424 RI and IR 431 CL as a function of different levels of fertilization. After the harvest, the grains were subjected to the debarking and polishing processes. Quality analysis was performed according to the physical classification of rice. The cultivar IR 424 RI obtained higher values of yellow, burned and plastered grains, and in the cultivar IR 431 LC more broken, chopped and stained grains were observed. The average yield of polished rice of cultivar IR 431 CL was 44.54% and cultivar IR 424 RI was 56.80%. Meanwhile, the average yield of whole rice of cultivar IR 431 CL was 65.02% and cultivar IR 424 RI was 73.08%. The increase in the fertilization levels of the rice influenced positively on the yield of whole grains, but did not present satisfactory results regarding the physical quality.
Redução da capacitância de barramento em driver para alimentação de LEDS
Authors: Yousef Eyad Yousef Ibrahim , Luz Paulo César Vargas
Published: March 2020

This paper presents the performance evaluation of control techniques for the reduction of capacitors in drivers for actuation LED.Through the active control of the driver output current, the ripple from the PFC stage of the current can be controlled, keeping itat levels appropriate to the technical standards, being IEC 61000-3-2 Class C and IEEE 1789. This control allows the outputvoltage ripple to vary at higher amplitude levels, allowing the use of a lower capacitance bus capacitor, therefore longer life usefuland reliability.The analyzed electronic system consists of the Buck-Boost converter was performed at the power control stagefor different bus voltages and output power. Different controller structures were analizes, in order to obtain an analysis of theinfluence of these controllers in the reduction of bus capacitance. Thus obtaining a greater ripple in the bus voltage, maintainingagreement with IEEE 1789 standard regarding current ripple limits on LEDs.
Medidas de temperatura em ambiente interno usando a Plataforma Arduino
Authors: Ritter Greice Scherer , Cavalheiro Eliezer Oliveira , Silva Ronaldo Barcelos e , Schmidt Leonardo Da Rosa , Maldaner Silvana
Published: March 2020

The paper presents the results of a study with temperature measurements using low cost sensors connected to an Arduino microcontroller. To perform the study, three sensors widely used for monitoring environmental conditions with Arduino. The selected sensors were the LM35DZ (analog sensor) and DHT11 and DHT22 (digital sensors). The LM35DZ sensor is a sensor known to be an analog sensor that has linear temperature response with voltage. The DHT11 sensor measures temperature and humidity simultaneously.  To measure temperature the DHT11 sensor uses a temperature-sensitive resistor and has a measurement range from 0 to 50 °C, with an uncertainty ± 2% ° C. The DHT22 has a measurement range  -40 to 80 ° C and an uncertainty ± 1% ° C.  Simultaneous temperature measurements with the three sensors showed good performance in indoor situations, showing the maximum and minimum temperatures of a daily temperature cycle.
PacMaze: um jogo de lógica utilizando conceitos de labirintos para um arcade de jogos educativos
Authors: Maran Vinícius , Costa Pedro Henrique Unterberger da , Silveira Luan Willig , Dutra Diones De Vargas
Published: March 2020

Computer programming is increasingly present in our daily lives, as a consequence of the number of people interested in this area is increasing proportionally. The intrinsic factor of programming is logical reasoning, where its scarcity results in poor performance for example in mathematics, physics and programming itself. The application of this game is to make the player develop a quick and logical thinking contributing in diverse areas of knowledge through the Ludum pro Bono game platform, a game platform focused on different methods of teaching through games.
Levantamento de plantas daninhas em lavoura de arroz irrigado com pivô em Cachoeira Do Sul - RS
Authors: Machado Bruno Luan Da Rosa , Schmidt Diogo André , Robattini Jean Carlos , Grohs Mara , Frescura Viviane Dal-souto
Published: March 2020

The survey of weed species in crops allows the identification, quantification of weed flora, its evolution in the area and helps in decision making for control of species. In this sense, the objective of this work was to survey weeds in pivot-irrigated rice fields in Cachoeira do Sul, Capané district, in the experimental area of the Rio Grandense do Arroz Institute (IRGA). Evaluations were performed by applying the 0.25 m2 square method, randomly launched sixty-eight times. Weeds in the sampled areas were removed from the soil, species identified and quantified. The evaluations were performed after the application of Gamit (preemerging), Ricer and Clincher and Imazethapyr (postemerging). Even after herbicide applications, 13 species were identified, representing the families Asteraceae, Poaceae, Malvaceae and Rubiaceae. Knowledge of the weeds present in the crop allows planning to improve control, avoiding unnecessary expense with herbicides or even using other methods to control these species.
Linfoma não-Hodgkin, manifestação no sistema estomatognático – Relato de caso clínico
Authors: Gomes Rita Fabiane Teixeira , Piazza José Luiz , Castelo Edilson Fernando
Published: March 2020

Linfomas são transformações neoplásicas de células linfoides, o Linfoma Não-Hodgkin raramente ocorre na cavidade oral, os casos intraorais podem manifestar-se como inchaço, dor, dormência, mobilidade dentária ou linfadenopatia cervical, os achados radiográficos podem assemelhar-se a osteomielites, neoplasias secundárias ou lesões odontogênicas. Pode ocorrer aumento de volume em tecido mole e não cicatrização após extrações dentárias - sinais suspeitos de malignidade. Os linfomas são lesões malignas agressivas, com alto potencial de cura através de quimioterapia associada ou não à radioterapia, se diagnosticados precocemente.  A cirurgia geralmente não é indicada. O prognóstico é bom para doenças localizadas, sendo menos favorável para doença disseminada. Cabe ao cirurgião-dentista diagnosticar manifestações estomatognáticas, contribuindo para o tratamento médico efetivo. O objetivo deste estudo é o relato de caso clínico de paciente com lesão osteolítica na mandíbula, com diagnóstico de Linfoma não-Hodgkin difuso de grandes células B a partir da investigação clínica multidisciplinar.
Programas de exercício físico para pessoas pré-hipertensas: revisão com base no modelo RE-AIM
Authors: Squarcini Camila Fabiana Rossi , Silva Andreza Nascimento da , Bastos Junior Clovis Lemos , Macedo Danielle De Amaral , Souza Joslei Viana de
Published: March 2020

Há evidências em estudos com alto controle de variáveis e grupos homogêneos (validade interna) de que o exercício físico reduz os índices pressóricos em pessoas com pré-hipertensão (PH). Entretanto, não se sabe ao certo se esses estudos apresentam informações suficientes para que tais exercícios sejam aplicados na prática clínica, no dia-a-dia (validade externa). Objetivo: O objetivo foi avaliar se os artigos científicos sobre programas de exercício físico para pessoas com pré-hipertensão da principal base de dados biomédica apresentam informações suficientes sobre a sua validade interna e externa. Métodos: Foi realizada uma revisão sistemática no PubMed entre 2007 a 2017, de artigos publicados inglês, espanhol ou português. Para seleção dos artigos, dois pareceristas (e um terceiro em caso de discordância) avaliaram o título, o resumo e o texto completo. Após esta etapa, foi realizada a avaliação da validade interna e externa utilizando o check-list do modelo de avaliação RE-AIM. Resultados: Foram encontrados 11.693 artigos para leitura dos títulos. Destes, 7 foram selecionados para leitura dos resumos, 5 artigos para leitura na íntegra, finalizando o processo com 3 artigos selecionados para a análise pelo modelo RE-AIM. Dos artigos selecionados, observou-se que os artigos atenderam apenas 33,33% dos itens das dimensões Alcance (público alvo e participante da pesquisa) e Implementação. No check-list da dimensão Efetividade/Eficácia (resultados) apenas 12,5% foram atendidos. As dimensões Adoção (organização e equipe alvo e participante) e Manutenção não foram contempladas. Conclusão: os artigos apresentam poucas informações a respeito da validade externa, dificultando assim a possibilidade de disseminação desses protocolos de exercício físico para outras realidades.
Extravasamiento de doxorubicina em punto de mano y tratamiento – Relato de caso
Authors: Fraga Lívia Ruas Meirelles , Cardoso Patrícia , Moraes Liliana De Los Santos , Carvalho José Antônio Mainardi , Bairros André Valle de
Published: March 2020

El cáncer de mama es una de las principales causas de mortalidad en la población femenina brasileña y la doxorubicina es uno de los medicamentos utilizados para evitar la replicación de células tumorales. Nuestro relato describe una paciente con neoplasia maligna de mama que sufrió el extravase de este fármaco durante el procedimiento de infusión. La evolución de la lesión en la mano de la paciente, el tratamiento y el pronóstico se evaluaron en este caso.
Efeitos da terapia aquática na qualidade do sono, algia e qualidade de vida em mulheres com fibromialgia
Authors: Schlemmer Géssica Bordin Viera , Maciel Mariane Brondani , Giotto Mai Carla Mirelle , Biazus Jaqueline De Fátima
Published: March 2020

Objetivo: O objetivo desta pesquisa foi avaliar os efeitos da terapia aquática na qualidade do sono, na qualidade de vida e no quadro álgico de mulheres com fibromialgia. Método: Esta pesquisa possui abordagem quantitativa. Para avaliar a qualidade de vida e a qualidade do sono foram utilizados os questionários QIF e PITTSBURGH, respectivamente. Para avaliar os pontos dolorosos foi utilizada a palpação dos triggers points da fibromialgia. Resultados: Verificou-se que a terapia aquática, os pontos de algia, qualidade do sono e de vida não foram estatisticamente significativas, porém muitas mudanças com relação a esses quesitos foram encontradas. Conclusão: Portanto as alterações emocionais sofridas por essas mulheres alteraram mais diretamente nas suas qualidades de vida, de sono e dor do que os benefícios proporcionados pelo protocolo de terapia aquática.
Descarte de medicamentos: avaliação do conhecimento dos produtores da atividade leiteira
Authors: Batista Nadine Neto , Mendes Graziella Alebrant , Carvalho Themis Goretti Moreira Leal de , Negrete Bruna Barcellos , Bulegon Jovana Simonetti , Schäfer Marcos Guilherme , Martins Valeska , Deuschle Viviane Cecília Kessler Nunes
Published: March 2020

O objetivo desse estudo foi avaliar a prática do uso e do descarte de medicamentos entre produtores da rede leiteira da região noroeste do Estado do Rio Grande do Sul.  O estudo foi conduzido mediante a aplicação de um questionário e a amostra foi constituída por 46 participantes. Os resultados demonstram que a maioria dos participantes apresenta medicamentos em suas residências (98%). Destes, 66% afirmam usar medicamentos por conta própria, 22% reutiliza medicamentos de tratamentos anteriores, 65% realiza o descarte em lixo comum e 9% descarta em vaso sanitário. Em relação à “farmácia caseira” o medicamento mais armazenado foi o paracetamol (78%). A maioria dos participantes (74%) relataram não ter recebido nenhuma informação a respeito do assunto. Neste sentido, observa-se que a maioria da população descarta os medicamentos de forma inadequada e a falta de orientações é o principal fator que leva a esta prática.
Adesão à terapia nutricional por pacientes obesos com e sem comorbidades de um ambulatório de Pelotas/RS
Authors: Vieira Juliana Bilhalva , Valle Sandra Costa , Ramos Camila Irigonhé
Published: March 2020

Objetivo: O objetivo desta pesquisa foi identificar a frequência de adesão ao tratamento nutricional de pacientes obesos com ou sem comorbidades atendidos no Ambulatório de Nutrição de acordo com fatores sociodemográficos e comportamentais. Métodos: Estudo transversal a partir das anamneses realizadas pelo Ambulatório de Nutrição da Faculdade de Nutrição (FN) da Universidade Federal de Pelotas (UFPel). Foram avaliados dados referentes à pacientes adultos, de ambos os sexos, obesos com ou sem a presença de comorbidades que consultaram no Ambulatório de Nutrição da FN/UFPel durante o ano de 2016. A realização deste estudo deve-se a grande dificuldade em manter satisfatórias taxas de adesão ao tratamento nutricional da obesidade, tendo em vista que o tratamento envolve mudanças comportamentais e no estilo de vida dos pacientes. Resultados: Os pacientes obesos assistidos no ambulatório eram predominantemente mulheres de baixa escolaridade, com idade superior a 30 anos, sedentárias, com presença de comorbidades e que apresentaram significativa redução do peso corporal. Conclusão: O atual estudo considerou que a adesão ao tratamento foi efetuada por aqueles que reduziram o peso corporal, o que ocorreu para uma porcentagem expressiva de pacientes e de forma significativa. Conclui-se que os pacientes obesos atendidos em ambulatório mostraram boa adesão ao tratamento nutricional, visto que estes obtiveram redução do peso corporal, mas a presença de comorbidades não foi associada ao grau de obesidade e a fatores sociodemográficos nesta população especifica.
Atividade física de crianças e apoio familiar percebido: um estudo exploratório
Authors: Silva Naildo Santos , Mello Julio Brugnara , Pedretti Augusto , Gaya Adroaldo , Gaya Anelise
Published: March 2020

Objetivo: O objetivo deste estudo é descrever a prevalência de crianças na zona saudável e risco para a atividade física e do apoio familiar percebido de acordo com o sexo e identificar as relações entre a atividade física diária e o apoio familiar percebido. Método: Trata-se de um estudo exploratório, com amostra voluntária de 49 crianças matriculadas no 5º ano do ensino fundamental. Foram avaliadas a atividade física através de pedometria e o apoio familiar através de questionário. Foram utilizadas análises descritivas e de associação. Foi testada a diferença entre os sexos com o teste qui-quadrado de Pearson. Resultados: Nas variáveis do apoio familiar percebido, não foram identificadas diferenças entre os sexos. Na análise bruta apenas a variável “seus pais (pai/mãe) levam você para praticar atividade física” se associou com a atividade física das crianças. No modelo final de análise ajustada, as variáveis “considera o pai ativo”, “mãe estimula a ser ativo” e “seus pais levam você para praticar atividade física” se associaram com a atividade física das crianças. Conclusões: As figuras maternas e paternas parecem assumir diferentes papéis no apoio percebido pelas crianças, assim sugerimos que o pai e a mãe (ou figuras equivalentes) devem fazer parte do processo das ações de intervenções para que estas relações sejam confirmadas de forma mais fidedigna.
Caracterização das dissertações e teses brasileiras acerca das arboviroses Dengue, Zika e Chikungunya
Authors: Silva Bárbara Coeli Oliveira da , Costa Francisco Hudson da Rocha , Barreto Vanessa Pinheiro , Dossantos Fernanda Nahara Carvalho , Neco Klebia Karoline dos Santos , Feijão Alexsandra Rodrigues
Published: March 2020

O estudo objetivou caracterizar as dissertações e teses brasileiras acerca das arboviroses dengue, zika e chikungunya. Trata-se de um estudo documental de abordagem quantitativa. A coleta de dados ocorreu no mês de outubro de 2018 no Catálogo de Teses e Dissertações da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). A amostra final foi de 189 estudos, 133 (70,38%) dissertações e 56 (29,62%) teses. As universidades que mais produziram sobre a temática foram a Fundação Oswaldo Cruz, com 27 pesquisas (14,28%) e a Universidade de São Paulo, com 23 (12,17%) estudos. Foram elencadas as seguintes categorias temáticas em maior destaque: Epidemiologia das Infeções pelos Arbovírus com 45 estudos (23,81%); caracterização molecular dos arbovírus, 20 (10,57%); e métodos de combate aos arbovírus, com 19 (10,05%) estudos. A totalidade de estudos analisados evidencia a necessidade de uma melhor compreensão acerca das arboviroses, no que se refere aos seus respectivos métodos de diagnósticos e tratamento. Sugere-se a realização de novos estudos e estímulo à pesquisa em todas as regiões do Brasil, de forma descentralizada e direcionada ao combate e mitigação das arboviroses.
A TRANSPARÊNCIA COMO INSTRUMENTO DE ACOMPANHAMENTO DO PLANEJAMENTO ESTRATÉGICO PÚBLICO MUNICIPAL
Authors: Fenner Vanessa Unfried , Scheid Liara Laís , Dalcin Dionéia , Anes Carlos Eduardo Ruschel
Published: March 2020

O acompanhamento do planejamento estratégico é uma etapa fundamental para garantir o seu sucesso, seja nas organizações privadas ou no setor público. Para auxiliar no monitoramento dos planos estratégicos, as organizações, em especial as públicas, contam com a transparência como uma de suas ferramentas. É através dela que as informações acerca da gestão pública, isto é, das ações previstas pelo planejamento, são disponibilizadas à sociedade e esta, ao acessá-las, poderá cobrar o cumprimento das metas e objetivos traçados. Esse controle social auxilia os agentes públicos a realizar as correções, atualizações e mudanças de curso necessárias para atingir seus objetivos estratégicos com maior efetividade. Nesse sentido, este ensaio teórico tem como objetivo discutir o papel da transparência como instrumento do planejamento estratégico público municipal, em especial, na etapa de acompanhamento. Neste estudo, evidencia-se o estágio de monitoramento dos planos estratégicos da administração pública. A partir da discussão, nota-se a importância dos mecanismos de transparência para o planejamento estratégico municipal, tendo em vista não somente o melhor funcionamento das instituições públicas, mas também o consequente aumento da qualidade de governança e da confiança no governo. Entende-se também que a transparência é promotora da participação dos cidadãos na gestão pública, o que favorece a efetividade das políticas públicas nesta elaboradas e implementadas e, consequentemente, impulsiona o desenvolvimento da sociedade.
Integration of Immunotherapy Into the Treatment of Advanced Urothelial Carcinoma
Authors: Ghatalia Pooja , Plimack Elizabeth R.
Published: March 2020

Five new PD-1/PD-L1 checkpoint inhibitors have been approved for the treatment of metastatic urothelial carcinoma (UC): pembrolizumab, atezolizumab, durvalumab, nivolumab, and avelumab. Although cisplatin-based chemotherapy remains the recommended frontline option for cisplatin-eligible patients with metastatic UC, immunotherapy is now an available option in the second-line setting as well as the frontline setting for selected cisplatin-ineligible patients who are either unable to tolerate chemotherapy or PD-L1–positive. This review describes the updated clinical efficacy of these checkpoint inhibitors in the treatment of advanced UC and suggests how they can be sequenced in the context of available chemotherapeutic options.
Influence of the temperature during the larval stage of development on lifespan in Drosophila melanogaster
Authors: Karaman A. S. , Vaiserman A. M. , Pisaruk A. V. , Koshel N. M. , Mekhova L. V. , Kozeretska I. A.
Published: March 2020

Aim. To study the influence of different temperatures on larval stage on the development duration and life expectancy of Drosophila melanogaster imago. Methods. The statistical significance of the indicators was determined by one-way ANOVA followed by Tukey HSD post-hoc tests to evaluate significance of differences between groups. Results. The development duration of imagoes significantly increased by 1.7 times when the developmental temperature decreased from 27.5 to 20.0°С. The average and maximum lifespan of the flies was maximum at a temperature of 22.5°С. The lifespan of fruit flies was significantly decreased compared to control (25.0°С) at a developmental temperature of 20.0°C and above 22.5°C. Conclusions. The obtained data suggest that developmental temperature significantly affects the lifespan of the flies. It likely is a physiological optimum of the temperature of development, in which life expectancy is maximal. Probably, this is due to the fact that at optimal temperature, the Drosophila development is most complete and their viability is highest.Keywords: Drosophila melanogaster, development, lifespan, temperature, larval stage.
Genotyping of the site of the mtDNA of representatives of the Cetoniinae subfamily
Authors: Balashenko N. A. , Semenyak A. A. , Kornilkova A. S. , Sen'kevich I. A. , Prishchepchik O. V. , Dromashko S. E.
Published: March 2020

Aim. Description of the results of molecular genetic research of insects-representatives of the subfamily Cetoniinae (flower chafer). Methods. For the analysis, methods of low-traumatic sampling of material for DNA isolation, polymerase chain reaction method, sequencing of the region of mtDNA COI gene were used. Results. A technique for sampling biological material for isolating DNA from the hind legs of individuals was proposed, which allowed not to remove from the habitat the individuals of the most valuable species, collected on the territory of the Republic of Belarus. A collection of DNA samples of representatives of the subfamily Cetoniinae was prepared. The haplotypes Oxythyrea funesta (Poda, 1761) and Protaetia aeruginosa (Linnaeus, 1767) of representatives of the populations inhabiting the territory of Belarus have been identified. Conclusions. There were significant differences in haplotypes among representatives of the Belarusian and European parts of their areal.

Keywords: molecular markers, mtDNA, DNA-barcoding, insects, subfamily Cetoniinae.
The protein expression changes during the signaling systems interaction in stressed pea seedlings
Authors: Nesterenko O. G. , Litvinov S. V. , Rashydov N. M.
Published: March 2020

Aim. The plant’s signaling systems functioning under stress impact is expressed in changes of the genes expression and protein synthesis. The purpose of this study is to investigate the effect of stressors and their combinations on pea seedlings at the molecular level, qualitative and quantitative changes in the spectrum of plant proteins. Methods. This phenomenon were investigated on four experimental groups: control seedlings, plants irradiated with gamma rays in the dose of 10 Gy, pea exposed to salt stress (0.22 Mol/L NaCl solution) and both stressors consequently. Proteins were isolated from each group and analyzed using ultra-high performance liquid chromatography. Results and conclusions. We observed the modification of expression of eight identified proteins: transketolase, malate dehydrogenase (43.52 kDa and 41.98 kDa), translation elongation factor EF-2 subunit, 14-3-3-like protein, heat shock cognate protein 80, heat shock cognate 70 kDa-like, 14-3-3-like protein B. Their significant role in the stress signals transduction and in the processes of forming an active response to the adverse factors is confirmed by concentration fluctuations between groups. The largest number of proteins has changed in response to the combined effect of ionizing radiation and salinity. Each factor by itself cause changes in less quantity of proteins.Keywords: signaling systems, proteins, pea seedlings, stress factors.
Genome specificity of primers to puroindoline genes
Authors: Sozinova O. I. , Kozub N. A. , Sozinov I. A. , Blume Ya. B.
Published: March 2020

Aim. Genome specificity of primers for amplification of complete coding sequences of puroindoline genes was studied. Methods. PCR with gene-specific primers was performed for amplification of puroindoline genes of wheat and related species. Results. The primer pair designated PinaDH is not gene-specific as it yields two products of amplification of the genes Pina-1 and Gsp-1. This primers pair is not specific for the Pina-1 gene of the genome V of D. villosum. The primer pairs designated PinaCM and Pinb are gene-specific as they produce one amplification product of respective length. We have demonstrated that the PinaCM primer pair is also specific for the genomes D, E and V, and the Pinb primer pair is not specific for the genome V. Conclusions. Gene and genomic specificity of primers for puroindoline genes was refined. Primers suitable for further direct sequencing of coding parts of puroindoline genes of wheat and related species were chosen.

Keywords: puroindoline, Triticum aestivum, Aegilops, Dasypyrum, genome specificity.
Agrobacterium-mediated in planta genetic transformation of winter wheat (Triticum aestivum L.)
Authors: Mykhalska S. I. , Komisarenko A. G. , Kurchii V. M. , Tishchenko O. M.
Published: March 2020

Aim. To optimize the agrobacterium-mediated method of winter wheat transformation (Triticum aestivum L.); to select the conditions and period of inoculation to effectively transfer the genes during pollination. Methods. Agrobacterium-mediated in planta genetic transformation of winter wheat (Triticum aestivum L.) during pollination. Results. The conditions for agrobacterium-mediated transformation method of winter wheat during natural (frequency pollination was 1 %) and non-natural (frequency pollination was 4 %) pollination were defined. Conclusions. The possibility of integrating transgenes into the genome of winter wheat plants by the method of Agrobacterium-mediated transformation in planta in the process of forced and natural pollination is demonstrated. It is found that the transformation efficiency to a large extent depends on the plant genotype and the method of carrying out the transformation procedure. The selection of transgenic plants under water deficit conditions allowed to identify the plants with functional transgene. The signs of functioning transgene have been remaining in the next generation of genetically modified winter wheat.

Keywords: Triticum aestivum L., Agrobacterium-mediated transformation in planta, transgenic plants, seeds.
Presowing radiation exposure of Chamomilla recutita L. seeds affects on flavonoid accumulation
Authors: Pchelovska S. A. , Litvinov S. V. , Shylina Yu. V. , Lystvan K. V. , Zhuk V. V. , Sokolova D. O. , Tonkal L. V. , Salivon A. G. , Nesterenko O. G.
Published: March 2020

Aim. Presowing radiation exposure of seeds is offered to apply as modification factor of secondary metabolites accumulation in medicinal plants. Aim of studying was to choose doses of both gamma- and X-ray exposure of Chamomilla recutita L. seeds that would lead to increasing synthesis of secondary metabolites and biomass. Methods. Both X- and gamma-radiation exposure of seeds, extraction of flavonoids, spectrophotometry assessment of flavonoid extracts, qualitative and semi-quantitative high effective liquid chromatography. Results. Presowing radiation exposure of Chamomilla recutita L. seeds increased both flavonoid production over control and yield of pharmacologically valuable raw material – inflorescence number per square. Chromatographical analyses indicated stable qualitative composition of Chamomilla recutita L. ethanol extracts; the exposure did not lead to synthesis of de novo components. Conclusions. Obtained data validate approach to apply presowing radiation exposure of Chamomilla recutita L. seeds (5–50 Gy dose rate) as modification factor increasing both its productivity and pharmaceutical value of medicinal plants.

Keywords: Chamomilla recutita L., stimulating productivity, flavonoids, presowing exposure.
Noncanonical DNA-binding properties of potential components of an antitumor composition (cytokine AIMP1/p43, lectin SNA, izatizon), which are able to affect different targets
Authors: Tsuvariev O. Yu. , Starozhuk O. V. , Karpova I. S. , Palchykovska L. G. , Zayika L. A. , Shyrina T. V. , Lylo V. V. , Kornelyuk O. I.
Published: March 2020

Aim. To search the DNA-binding properties of various drugs promising for the creation of the antitumor composition (AIMP1/p43, SNA-I, izatizon), as well as their ability to influence on key matrix processes – DNA transcription and replication, which are a sensitive target for the action of many pharmacological drugs. Methods. The recombinant protein AIMP1/p43 was obtained from supernatant of E.coli lysed cells by metallic chelating chromatography on a Ni‑NTA‑agarose column. Also there were used the elderberry bark lectin SNA-I (LECTINOTEST, Ukraine), and the preparation of own production izatizon (IMBG NAS of Ukraine). The DNA-binding ability of the drugs was investigated by EMSA. As a transcription test system, an enzyme complex of DNA-dependent RNA polymerase of T7 bacteriophage and plasmid DNA pTZ19R * were used. PCR with Taq DNA polymerase and the plasmid pTZ19R * was used as the model replicative system. Results. Experimental conditions, in which the specificity of each drug action appeared, were found: the cytokine AIMP1/p43 and the SNA-I demonstrated the ability to bind to DNA by the EMSA test; izatizon induced structural changes in DNA, blocked the amplification of DNA during the PCR process, and also in vitro transcription with the participation of DNA-dependent RNA polymerase of the bacteriophage T7. Conclusions. Each of the selected drugs, which according to the literature data have antitumor potential, has demonstrated the ability to interact with the model supercoiled plasmid DNA pTZ19R *. The different ways of the selected drugs influence on DNA allows us to expect their effective combined action and synergism in case of their application as a common antitumor composition.

Keywords: cytokine AIMP1/p43, SNA-I, izatizon, EMSA method, transcription inhibiting, amplification blocking.
Transcriptional factor Cat8 is involved in regulation of xylose fermentation in engineered Saccharomyces cerevisiae
Authors: Dzanaieva L. S. , Dmytruk K. V. , Sybirny A. A.
Published: March 2020

Aim. The aim of this work is the construction of cat8Δ strain on the base of xylose-fermenting S. cerevisiae strain and evaluation of the xylose fermentation rate. Methods. The CAT8 deletion cassette harboring natNT2 gene flanking with 5’ and 3’ non-coding regions of CAT8 gene has been constructed. After transformation by the cassette the cat8Δ strain was selected on the nourseothricin containing medium. Xylose fermentation experiments of constructed strain was performed in mineral medium supplemented with xylose under oxygen-limited conditions. Results. Xylose-fermenting cat8Δ S. cerevisiae strain has been constructed by homologous recombination of the CAT8 deletion cassette with target sequences in the genome of GS010 strain. The cat8Δ strain possessed increase in ethanol accumulation, ethanol yield, rate of ethanol production and productivity of ethanol synthesis relative to the parental GS010 strain for 9.5, 6, 20 and 12 %, respectively. Conclusions. The mutant of the xylose-fermenting S. cerevisiae strain with knock out of the CAT8 gene coding for transcriptional activator, has been constructed. The cat8Δ mutant showed 9.5 % increase in ethanol production from xylose relative to parental strain.

Keywords: alcoholic fermentation, xylose, S. cerevisiae, Cat8.
The analisis of human MGMT gene orthologous in protests
Authors: Pidpala O. V. , Lukash L. L.
Published: March 2020

Aim. The intron sequences of orthologous О6-methylguanin-DNA methyltransferase (MGMT) genes in Protists on the early stages of their formation in eukaryotic organisms have been analysed. Methods. Homologous regions have been defined by the program BLASTN 2.6.1. Nucleotide sequences of the bacterial and mitochondrial group II introns have been taken from Database for Bacterial Group II Introns. Searching and identifying the MGEs have been realized by using CENSOR. Results. It has been shown that the evolution of the gene does not always coincide with the evolution of the organism. This is shown on the example of intron loss and gain in social amoebae Dictyostelium. Also it has been found the fragmentary nature of homology between various introns and exons of the orthologous genes. Conclusions. The obtained results allow offer a suggestion about the endogenous mosaic character of the evolutional formation of the gene structural units. Keywords: О6-methylguanin-DNA methyltransferase (MGMT) gene orthologous, Protists, gene evolution, spliceosomal introns, intron loss and gain.
Analysis of Glu-1 loci in samples of rare wheat species and their hybrides
Authors: Sichkar S. M. , Velykozhon L. H. , Dubrovna O. V. , Morgun B. V.
Published: March 2020

Aim. Determination of the allelic composition of Glu-1 loci in samples of rare wheat species and their hybrids with spring bread wheat. Methods. PCR analysis. Results. In existing collection samples of the rare wheat species the alleles of the a/c loci Glu-A1 were found, while allele b was found only in T.dicoccum, var. volgense (Emmer Kokchetavskaya). An additional amplicon with a length of 450 bp was found in the Glu-A1 locus in the sample (T. dicoccum × Dasypyrum villosum), and in (Ae. ventricosa × T. dicoccum) another one a length of 700 bp in genome B, was found require further research. Among hexaploid wheat, it was revealed genotypes differing in the presence of alleles a and d of the locus Glu-D1. The hybridity of the received forms has been confirmed, as evidenced by the identification of both parent components in the hybrids or alleles of the Glu-D1 locus of bread wheat in hybrids with emmer. Conclusions. Analysis of the allele composition of Glu1 locuses in samples of rare wheat species and their hybrids with bread wheat allowed to select genotypes and hybrid combinations that may be promising for further breeding work.

Keywords: Triticum spelta L., T. dicoccum, hybrids, PCR analysis, Glu-1 locus.