bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of wind energy production on a threatened species, the Bicknell’s Thrush Catharus bicknelli, with and without mitigation
Authors: LEMAÎTRE JÉRÔME , LAMARRE VINCENT
Published: March 2020

Renewable energy helps meet the growing energetic demand while reducing greenhouse gas emissions. Despite its environmental benefits, production of wind energy can adversely affect wildlife populations, including birds. In some species, indirect impacts such as habitat loss and disturbance may be more important than fatalities caused by collisions with turbines. Bicknell’s Thrush Catharus bicknelli, one of the most endangered bird species in North America, may be threatened by wind energy production because it breeds at high elevation sites, which are often prized for their wind potential. Our study had two objectives: we first aimed to document the impacts of the construction and operation of a wind energy facility without mitigation strategy on the occurrence of the Bicknell’s Thrush. At a second facility, we then tested the effectiveness of turbine micro-siting as an effective mitigation strategy to reduce the impacts of wind-energy development on the species. We conducted avian point-counts at 143 locations spread across both facilities in Quebec (Canada) at different periods: before, during and after construction. We modelled the probability of occurrence of the species at point-counts as a function of period, forest loss caused by wind energy development, distance to the nearest turbine and habitat suitability. At the facility without mitigation, we found that the probability of occurrence decreased during construction and early operation at high elevation sites, where most of the turbines were erected. However, the Bicknell’s Thrush recolonized high elevation sites eight years post-construction. In addition, we did not detect a significant impact of wind energy production on the species’ occurrence at the facility where micro-siting was applied. We conclude that habitat loss and disturbance during construction are the main impacts of wind energy production on the Bicknell’s Thrush and that micro-siting appears to be a promising mitigation strategy.
Lean body mass is the strongest anthropometric predictor of left ventricular mass in the obese paediatric population
Authors: Shea James R. , Henshaw Melissa H. , Carter Janet , Chowdhury Shahryar M.
Published: March 2020

Background:

Indexing left ventricular mass to body surface area or height2.7 leads to inaccuracies in diagnosing left ventricular hypertrophy in obese children. Lean body mass predictive equations provide the opportunity to determine the utility of lean body mass in indexing left ventricular mass. Our objectives were to compare the diagnostic accuracy of predicted lean body mass, body surface area, and height in detecting abnormal left ventricle mass in obese children.

Methods:

Obese non-hypertensive patients aged 4–21 years were recruited prospectively. Dual-energy X-ray absorptiometry was used to measure lean body mass. Height, weight, sex, race, and body mass index z-score were used to calculate predicted lean body mass.

Results:

We enrolled 328 patients. Average age was 12.6 ± 3.8 years. Measured lean body mass had the strongest relationship with left ventricular mass (R2 = 0.84, p < 0.01) compared to predicted lean body mass (R2 = 0.82, p < 0.01), body surface area (R2 = 0.80, p < 0.01), and height2.7 (R2 = 0.65, p < 0.01). Of the clinically derived variables, predicted lean body mass was the only measure to have an independent association with left ventricular mass (β = 0.90, p < 0.01). Predicted lean body mass was the most accurate scaling variable in detecting left ventricular hypertrophy (positive predictive value = 88%, negative predictive value = 99%).

Conclusions:

Lean body mass is the strongest predictor of left ventricular mass in obese children. Predicted lean body mass is the most accurate anthropometric scaling variable for left ventricular mass in left ventricular hypertrophy detection. Predicted lean body mass should be considered for clinical use as the body size correcting variable for left ventricular mass in obese children.
Impact of the superior cavopulmonary anastomosis on cerebral oxygenation
Authors: Thatte Nikhil , Zhou Lingyu , Kheir John N.
Published: March 2020

Background:

Patients with univentricular heart disease may undergo a superior cavopulmonary anastomosis, an operative intervention that raises cerebral venous pressure and impedance to cerebral venous return. The ability of infantile cerebral autoregulation to compensate for this is not well understood.

Materials and methods:

We identified all patients undergoing a superior cavopulmonary anastomosis (cases) and compared metrics of cerebral oxygenation upon admission to the ICU with patients following repair of tetralogy of Fallot or arterial switch operation (controls). The primary endpoint was cerebral venous oxyhaemoglobin saturation measured from an internal jugular venous catheter. Other predictor variables included case–control assignment, age, weight, sex, ischemic times, arterial oxyhaemoglobin saturation, mean arterial blood pressure, and superior caval pressure.

Results:

A total of 151 cases and 350 controls were identified. The first post-operative cerebral venous oxyhaemoglobin saturation was significantly lower following superior cavopulmonary anastomosis than in controls (44 ± 12 versus 59 ± 15%, p < 0.001), as was arterial oxyhaemoglobin saturation (81 ± 9 versus 98 ± 5%, p < 0.001). Cerebral venous oxyhaemoglobin saturation correlated poorly with superior caval pressure in both groups. When estimated by linear mixed effects model, arterial oxyhaemoglobin saturation was the primary determinant of central venous oxyhaemoglobin saturation in both groups (β = 0.79, p = 3 × 10−14); for every 1% point increase in arterial oxyhaemoglobin saturation, there was a 0.79% point increase in venous oxyhaemoglobin saturation. In this model, no other predictors were significant, including superior caval pressure and case–control assignment.

Conclusion:

Cerebral autoregulation appears to remain intact despite acute imposition of cerebral venous hypertension following superior cavopulmonary anastomosis. Following superior cavopulmonary anastomosis, cerebral venous oxyhaemoglobin saturation is primarily determined by arterial oxyhaemoglobin saturation.
Examining the roles of metacognitive beliefs and maladaptive aspects of perfectionism in depression and anxiety
Authors: Kannis-Dymand Lee , Hughes Emily , Mulgrew Kate , Carter Janet D. , Love Steven
Published: March 2020

Background:

Metacognition and perfectionism are factors found to be associated with both anxiety and depression. A common component that underlies these factors is the influence of perseverance, or the tendency to continue a behaviour or thought even if it is no longer productive.

Aims:

This study aimed to investigate the relationships between metacognitive beliefs with maladaptive aspects of perfectionism (i.e. perseverance behaviours), and their relation to anxiety and depression.

Method:

Participants (n = 1033) completed six self-report questionnaires measuring metacognitive beliefs about rumination and worry, perseverance, anxiety and depression. Data were analysed using correlational testing, and structural equation modelling.

Results:

Results of structural equation modelling revealed that positive metacognitive beliefs about repetitive negative thinking increased the likelihood to perceive the thinking as uncontrollable, and that perseverance behaviours were predicted by all metacognitive beliefs. Furthermore, examination of partial correlations revealed that both negative metacognitive beliefs about repetitive negative thinking and perseverance behaviours predicted anxiety and depression; however, negative metacognitive beliefs were the strongest predictor, in both cases.

Conclusions:

The results provided support for current metacognitive models, in that the interpretation of cognitive perseveration sequentially influences psychopathology, but also provided insight into the inclusion of perseveration behaviours. Furthermore, the findings may also have value in a clinical setting, as targeting metacognitive beliefs in the presence of perseverance type behaviours may prove beneficial for treatment.
A new tool for rating cognitive behavioural supervision – preliminary findings in a clinical setting
Authors: Moeller S.B. , Rosenberg N.K. , Hvenegaard M. , Straarup K. , Austin S.F.
Published: March 2020

Cognitive behavioural therapy (CBT) is an evidence-based psychotherapy and one of the most widely used treatments for mental health problems. It is generally acknowledged that supervision improves the quality of treatment although systematic descriptions and empirical evaluation of supervision have been sparse. Moreover, there are relatively few valid and reliable instruments to evaluate supervision. Based on a comprehensive review of the supervision literature, six competency domains were identified to cover the scope of CBT supervision: Theory, Focus, Learning strategy, Techniques, Structure, and Interpersonal style. The Moeller, Moerch, Rosenberg Supervision Scale (MMRSS) was developed to evaluate supervisor performance within each of these domains after observation of supervision. The present study examined the psychometric properties of the MMRSS (inter-rater reliability and construct validity), the clinical utility, and satisfaction when using MMRSS to evaluate CBT supervision. CBT supervisors (n = 8) were recruited for the study and provided videos of group supervision. A total of 21 videos were rated using the MMRSS and the Supervisory Competency Scale (SCS) by two independent raters. Supervisees and supervisors completed a satisfaction questionnaire to capture their experience of using the MMRSS during supervision of supervision. The MMRSS showed acceptable internal consistency and validity. Several domains in MMRSS (Structure, Learning strategy, and Interpersonal style) correlated significantly with the corresponding domains in the SCS for cognitive supervision. Preliminary results indicate that the MMRSS may be a valid and clinically useful tool to evaluate CBT supervision, although further systematic evaluation is needed.

Key learning aims

(1)

To understand that empirically founded evaluation of cognitive behavioural supervision is essential for good training.

(2)

To argue that a modern view of supervision places an emphasis on learning principles.

(3)

To describe the Moeller, Moerch, Rosenberg Supervision Scale (MMRSS) and the scale’s preliminary psychometric properties.

(4)

To describe the supervisors’ and supervisees’ reported satisfaction using the MMRSS.
Failed and successful attempts at institutional change: the battle for marriage equality in the United States
Authors: Mariani Giulia
Published: March 2020

By focusing on the legislative process underpinning marriage equality in the American states, this article identifies the combinations of conditions under which attempts at institutional displacement succeed or fail. Hitherto, few scholarly works have empirically examined displacement and whether, and how, actors can preserve institutional stability in the face of organized efforts to change institutions. Taking causal complexity into account, the analytical model factors in the resources of both change and status quo actors as well as the political context that enables or constrains their strategies. The results of the comparative analysis show that states have followed different paths to the displacement of heterosexual marriage in favor of marriage equality. Yet, most crucially, the findings pinpoint that the inclusion of religious exemption clauses is a conditionsine qua nonfor marriage equality laws to be effectively passed, thus challenging the widely accepted notion that morality policies are foreign to compromise.
SAR analysis of tri-band antennas for a 5G eyewear device
Authors: Geyikoğlu M. Dilruba , Koç Polat Hilal , Kaburcuk Fatih , Çavuşoğlu Bülent
Published: March 2020

The goal of this study is to analyze the specific absorption rate (SAR) distribution of the projected 5G frequencies below 6 GHz and at Wi-Fi frequency (2.45 GHz) on a human head, for eyewear device applications. Two separate tri-band printed dipole antennas for this purpose are designed and fabricated at operating frequencies of 2.45/3.8/6 GHz for prototype-1 and at operating frequencies of 2.45/3.6/4.56 GHz for prototype-2. In order to obtain the desired frequencies: first, the prototypes of the proposed antennas are fine-tuned via Computer Simulation Technology Microwave Studio (CST) and then fabricated on the FR4 layer. The reflection coefficient (S11) is tested and the simulation results are confirmed. In order to analyze the effect of wearing a pair of glasses' frame including a tri-band 5G antenna, a frame is designed and produced via 3D printer with polylactic acid material which has high dielectric constant (ɛr = 8.1). The SAR results of the proposed antennas have been examined for the cases where the antenna is embedded in the frame and is used alone. Both cases were analyzed by using the homogeneous specific anthropomorphic mannequin and the heterogeneous visible human head phantoms and the results have been evaluated in terms of SAR10 g values.
Reply to comment by Michard et al. on “The Mesozoic Margin of the Maghrebian Tethys in the Rif Belt (Morocco): Evidence for Polyphase Rifting and Related Magmatic Activity”
Authors: Gimeno‐Vives Oriol , Mohn Geoffroy , Bosse Valérie , Haissen Faouziya , Zaghloul Mohamed Najib , Atouabat Achraf , Frizon de Lamotte Dominique
Published: March 2020

Key Points

Opening of the Maghrebian Tethys during a major Middle Jurassic rifting phase

Late Triassic to Early Jurassic CAMP‐related magmatism

Non‐oceanic nature of the Mesorif Gabbroic Complex and absence of an overlaying oceanic sequence
Decreased vitamin C uptake mediated by SLC2A3 promotes leukaemia progression and impedes TET2 restoration
Authors: Liu Jun , Hong Junshik , Han Heejoo , Park Jihyun , Kim Dongchan , Park Hyejoo , Ko Myunggon , Koh Youngil , Shin Dong-Yeop , Yoon Sung-Soo
Published: March 2020

Background

Vitamin C suppresses leukaemogenesis by modulating Tet methylcytosine dioxygenase (TET) activity. However, its beneficial effect in the treatment of patients with acute myeloid leukaemia (AML) remains controversial. In this study, we aimed to identify a potential predictive biomarker for vitamin C treatment in AML.

Methods

Gene expression patterns and their relevance to the survival of AML patients were analysed with The Cancer Genome Atlas (TCGA) and Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database cases. In vitro experiments were performed on AML cell lines, a SLC2A3-knockdown cell line and patient-derived primary AML cells.

Results

SLC2A3 expression was significantly decreased in leukaemic blast cells. Below-median SLC2A3 expression was associated with poor overall survival. Low SLC2A3 expression was associated with less effective demethylation, and a diminished vitamin C effect in the AML and lymphoma cell lines. SLC2A3 knockdown in the KG-1 cell line decreased the response of vitamin C. In patient-derived primary AML cells, vitamin C only restored TET2 activity when SLC2A3 was expressed.

Conclusion

SLC2A3 could be used as a potential biomarker to predict the effect of vitamin C treatment in AML.
PfCERLI1 is a conserved rhoptry associated protein essential for Plasmodium falciparum merozoite invasion of erythrocytes
Authors: Liffner Benjamin , Frölich Sonja , Heinemann Gary K. , Liu Boyin , Ralph Stuart A. , Dixon Matthew W. A. , Gilberger Tim-Wolf , Wilson Danny W.
Published: March 2020

The disease-causing blood-stage of the Plasmodium falciparum lifecycle begins with invasion of human erythrocytes by merozoites. Many vaccine candidates with key roles in binding to the erythrocyte surface and entry are secreted from the large bulb-like rhoptry organelles at the apical tip of the merozoite. Here we identify an essential role for the conserved protein P. falciparum Cytosolically Exposed Rhoptry Leaflet Interacting protein 1 (PfCERLI1) in rhoptry function. We show that PfCERLI1 localises to the cytosolic face of the rhoptry bulb membrane and knockdown of PfCERLI1 inhibits merozoite invasion. While schizogony and merozoite organelle biogenesis appear normal, biochemical techniques and semi-quantitative super-resolution microscopy show that PfCERLI1 knockdown prevents secretion of key rhoptry antigens that coordinate merozoite invasion. PfCERLI1 is a rhoptry associated protein identified to have a direct role in function of this essential merozoite invasion organelle, which has broader implications for understanding apicomplexan invasion biology.
Kondo-free mirages in elliptical quantum corrals
Authors: Li Qili , Li Xiaoxia , Miao Bingfeng , Sun Liang , Chen Gong , Han Ping , Ding Haifeng
Published: March 2020

The quantum mirage effect is a fascinating phenomenon in fundamental physics. Landmark experiments on quantum mirages reveal atomic-scale transport of information with potential to remotely probe atoms or molecules with minimal perturbation. Previous experimental investigations are Kondo-effect based; the quantum mirages appear only near the Fermi energy. This strongly limits the exploration of the mechanism and potential application. Here we demonstrate a Kondo-free quantum mirage that operates in a wide energy range beyond Fermi energy. Together with an analytical model, our systematic investigations identify that the quantum mirage is the result of quantum interference of the onsite electronic states with those scattered by the adatom at the focus of elliptical quantum corrals, where two kinds of scattering paths are of critical importance. Moreover, we also demonstrate the manipulation of quantum mirages with pseudo basic logic operations, such as NOT, FANOUT and OR gates.
Directional ionic transport across the oxide interface enables low-temperature epitaxy of rutile TiO2
Authors: Park Yunkyu , Sim Hyeji , Jo Minguk , Kim Gi-Yeop , Yoon Daseob , Han Hyeon , Kim Younghak , Song Kyung , Lee Donghwa , Choi Si-Young , Son Junwoo
Published: March 2020

Heterogeneous interfaces exhibit the unique phenomena by the redistribution of charged species to equilibrate the chemical potentials. Despite recent studies on the electronic charge accumulation across chemically inert interfaces, the systematic research to investigate massive reconfiguration of charged ions has been limited in heterostructures with chemically reacting interfaces so far. Here, we demonstrate that a chemical potential mismatch controls oxygen ionic transport across TiO2/VO2 interfaces, and that this directional transport unprecedentedly stabilizes high-quality rutile TiO2 epitaxial films at the lowest temperature (≤ 150 °C) ever reported, at which rutile phase is difficult to be crystallized. Comprehensive characterizations reveal that this unconventional low-temperature epitaxy of rutile TiO2 phase is achieved by lowering the activation barrier by increasing the “effective” oxygen pressure through a facile ionic pathway from VO2-δ sacrificial templates. This discovery shows a robust control of defect-induced properties at oxide interfaces by the mismatch of thermodynamic driving force, and also suggests a strategy to overcome a kinetic barrier to phase stabilization at exceptionally low temperature.
Tunable rectification in a molecular heterojunction with two-dimensional semiconductors
Authors: Shin Jaeho , Yang Seunghoon , Jang Yeonsik , Eo Jung Sun , Kim Tae-Wook , Lee Takhee , Lee Chul-Ho , Wang Gunuk
Published: March 2020

Until now, a specifically designed functional molecular species has been recognized as an absolute necessity for realizing the diode’s behavior in molecular electronic junctions. Here, we suggest a facile approach for the implementation of a tailored diode in a molecular junction based on non-functionalized alkyl and conjugated molecular monolayers. A two-dimensional semiconductor (MoS2 and WSe2) is used as a rectifying designer at the alkyl or conjugated molecule/Au interface. From the adjustment of band alignment at molecules/two-dimensional semiconductor interface that can activate different transport pathways depending on the voltage polarity, the rectifying characteristics can be implemented and controlled. The rectification ratio could be widely tuned from 1.24 to 1.83 × 104 by changing the molecular species and type and the number of layers of the two-dimensional semiconductors in the heterostructure molecular junction. Our work sets a design rule for implementing tailored-diode function in a molecular heterojunction structure with non-functionalized molecular systems.
Dense active matter model of motion patterns in confluent cell monolayers
Authors: Henkes Silke , Kostanjevec Kaja , Collinson J. Martin , Sknepnek Rastko , Bertin Eric
Published: March 2020

Epithelial cell monolayers show remarkable displacement and velocity correlations over distances of ten or more cell sizes that are reminiscent of supercooled liquids and active nematics. We show that many observed features can be described within the framework of dense active matter, and argue that persistent uncoordinated cell motility coupled to the collective elastic modes of the cell sheet is sufficient to produce swirl-like correlations. We obtain this result using both continuum active linear elasticity and a normal modes formalism, and validate analytical predictions with numerical simulations of two agent-based cell models, soft elastic particles and the self-propelled Voronoi model together with in-vitro experiments of confluent corneal epithelial cell sheets. Simulations and normal mode analysis perfectly match when tissue-level reorganisation occurs on times longer than the persistence time of cell motility. Our analytical model quantitatively matches measured velocity correlation functions over more than a decade with a single fitting parameter.
Carboranyl Derivatives of Rofecoxib with Cytostatic Activity against Human Melanoma and Colon Cancer Cells
Authors: Buzharevski Antonio , Paskaš Svetlana , Sárosi Menyhárt-Botond , Laube Markus , Lönnecke Peter , Neumann Wilma , Murganić Blagoje , Mijatović Sanja , Maksimović-Ivanić Danijelа , Pietzsch Jens , Hey-Hawkins Evamarie
Published: March 2020

Owing to the involvement of cyclooxygenase-2 (COX-2) in carcinogenesis, COX-2-selective inhibitors are increasingly studied for their potential cytotoxic properties. Moreover, the incorporation of carboranes in structures of established anti-inflammatory drugs can improve the potency and metabolic stability of the inhibitors. Herein, we report the synthesis of carborane-containing derivatives of rofecoxib that display remarkable cytotoxic or cytostatic activity in the micromolar range with excellent selectivity for melanoma and colon cancer cell lines over normal cells. Furthermore, it was shown that the carborane-modified derivatives of rofecoxib showed different modes of action that were dependent on the cell type.
Publisher Correction: Femtosecond laser fabrication of silver nanostructures on glass for surface enhanced Raman spectroscopy
Authors: MacKenzie Mark , Chi Haonan , Varma Manoj , Pal Parama , Kar Ajoy , Paterson Lynn
Published: March 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Enzyme Release from Polyion Complex by Extremely Low Frequency Magnetic Field
Authors: Vlasova Kseniya Yu. , Vishwasrao Hemant , Abakumov Maxim A. , Golovin Dmitry Yu. , Gribanovsky Sergey L. , Zhigachev Alexander O. , Poloznikov Andrey А. , Majouga Alexander G. , Golovin Yuri I. , Sokolsky-Papkov Marina , Klyachko Natalia L. , Kabanov Alexander V.
Published: March 2020

Remote nano-magneto-mechanical actuation of magnetic nanoparticles (MNPs) by non-heating extremely low frequency magnetic field (ELF MF) is explored as a tool for non-invasive modification of bionanomaterials in pharmaceutical and medical applications. Here we study the effects of ELF MF (30–160 Hz, 8–120 kA/m) on the activity and release of a model enzyme, superoxide dismutase 1 (SOD1) immobilized by polyion coupling on dispersed MNPs aggregates coated with poly(L-lysine)-block-poly(ethylene glycol) block copolymer (s-MNPs). Such fields do not cause any considerable heating of MNPs but promote their rotating-oscillating mechanical motion that produces mechanical forces and deformations in adjacent materials. We observed the changes in the catalytic activity of immobilized SOD1 as well as its release from the s-MNPs/SOD1 polyion complex upon application of the ELF MF for 5 to 15 min. At longer exposures (25 min) the s-MNPs/SOD1 dispersion destabilizes. The bell-shaped effect of the field frequency with maximum at f = 50 Hz and saturation effect of field strength (between 30 kA/m and 120 kA/m at f = 50 Hz) are reported and explained. The findings are significant as one early indication of the nano-magneto-mechanical disruption by ELF MF of cooperative polyion complexes that are widely used for design of current functional healthcare bionanomaterials.
Evidence for population expansion of Cotton pink bollworm Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) in India
Authors: Naik V. Chinna Babu , Pusadkar Pratik P. , Waghmare Sandesh T. , K. P. Raghavendra , Kranthi Sandhya , Kumbhare Sujit , Nagrare V. S. , Kumar Rishi , Prabhulinga Tenguri , Gokte-Narkhedkar Nandini , Waghmare V. N.
Published: March 2020

Pink bollworm,Pectinophora gossypiella(Saunders) infestation onBtcotton is a major concern to cotton production in India. The genetic diversity and phylogeographic structure of the insect in light of PBW resistance needs to be revisited. The objective of this study was to identify different haplotypes of pink bollworm and their distribution in India. To achieve this we studied the population structure in 44 cotton growing districts of India. The partial mitochondrial COI sequence analyses of 214 pink bollworm populations collected from 44 geographical locations representing 9 cotton growing states of India were analysed. Genetic diversity analysis exhibited presence of 27 haplotypes, among them Pg_H1 and Pg_H2 were the most common and were present in 143 and 32 populations, respectively. Distributions of pairwise differences obtained with partial COI gene data from the overall Indian populations are unimodal, suggesting population expansion in India. Significant neutrality test on the basis of Tajima’ D and Fu’s Fs presented a star-shaped haplotype network together with multiple haplotypes. The unimodal mismatch distribution, rejection of neutrality test with significant negative values supported the theory of demographic expansion in cotton pink bollworm populations in India. Genetic data not only provides us with a perspective of population genetics, but also that the two populations of pink bollworm, those occurring early in the season are genetically close to the late season populations with respect to their partial CO1 region. Resistance to Cry toxins does not seem to have had an impact on this region of the mt DNA in populations of pink bollworm.
Observation of spin-polarized Anderson state around charge neutral point in graphene with Fe-clusters
Authors: Park Jungmin , Oh Inseon , Jin Mi-Jin , Jo Junhyeon , Choe Daeseong , Yun Hyung Duk , Lee Suk Woo , Lee Zonghoon , Kwon Soon-Yong , Jin Hosub , Chung Suk Bum , Yoo Jung-Woo
Published: March 2020

The pristine graphene described with massless Dirac fermion could bear topological insulator state and ferromagnetism via the band structure engineering with various adatoms and proximity effects from heterostructures. In particular, topological Anderson insulator state was theoretically predicted in tight-binding honeycomb lattice with Anderson disorder term. Here, we introduced physi-absorbed Fe-clusters/adatoms on graphene to impose exchange interaction and random lattice disorder, and we observed Anderson insulator state accompanying with Kondo effect and field-induced conducting state upon applying the magnetic field at around a charge neutral point. Furthermore, the emergence of the double peak of resistivity at ν = 0 state indicates spin-splitted edge state with high effective exchange field (>70 T). These phenomena suggest the appearance of topological Anderson insulator state triggered by the induced exchange field and disorder.
Multiplexed MRM-based protein quantification of putative prognostic biomarkers for chronic kidney disease progression in plasma
Authors: Makridakis Manousos , Kontostathi Georgia , Petra Eleni , Stroggilos Rafael , Lygirou Vasiliki , Filip Szymon , Duranton Flore , Mischak Harald , Argiles Angel , Zoidakis Jerome , Vlahou Antonia
Published: March 2020

Current diagnostic measures for Chronic Kidney Disease (CKD) include detection of reduced estimated glomerular filtration rate (eGFR) and albuminuria, which have suboptimal accuracies in predicting disease progression. The disease complexity and heterogeneity underscore the need for multiplex quantification of different markers. The goal of this study was to determine the association of six previously reported CKD-associated plasma proteins [B2M (Beta-2-microglobulin), SERPINF1 (Pigment epithelium-derived factor), AMBP (Protein AMBP), LYZ (Lysozyme C), HBB (Hemoglobin subunit beta) and IGHA1 (Immunoglobulin heavy constant alpha 1)], as measured in a multiplex format, with kidney function, and outcome. Antibody-free, multiple reaction monitoring mass spectrometry (MRM) assays were developed, characterized for their analytical performance, and used for the analysis of 72 plasma samples from a patient cohort with longitudinal follow-up. The MRM significantly correlated (Rho = 0.5–0.9) with results from respective ELISA. Five proteins [AMBP, B2M, LYZ, HBB and SERPINF1] were significantly associated with eGFR, with the three former also associated with unfavorable outcome. The combination of these markers provided stronger associations with outcome (p < 0.0001) compared to individual markers. Collectively, our study describes a multiplex assay for absolute quantification and verification analysis of previously described putative CKD prognostic markers, laying the groundwork for further use in prospective validation studies.
Hepamine - A Liver Disease Microarray Database, Visualization Platform and Data-Mining Resource
Authors: Itzel Timo , Neubauer Melanie , Ebert Matthias , Evert Matthias , Teufel Andreas
Published: March 2020

Numerous gene expression profiling data on liver diseases were generated and stored in public databases. Only few were used for additional analyses by the hepatology research community. This may mostly be due to limited bioinformatics knowledge of most biomedical research personnel. In order to support an easy translation of bioinformatics data into translational hepatology research, we created Hepamine, a liver disease gene expression, visualization platform and data-mining resource. Microarray data were obtained from the NCBI GEO database. Pre-analysis of expression data was performed using R statistical software and the limma microarray analysis package from the Bioconductor repository. We generated Hepamine, a web-based repository of pre-analyzed microarray data for various liver diseases. At its initial release Hepamine contains 13 gene expression datasets, 20 microarray experiments and approximately 400 000 gene expression measurements. A self-explanatory website offers open and easy access to gene expression profiles. Results are furthermore visualized in simple three-color tables indicating differential expression. All data were linked to common functional and genetic databases particularly through the DAVID bioinformatics suite. Hepamine provides comprehensive data and easy access to hepatologic gene expression data even without in depth bioinformatics or microarray profiling experience. http://www.hepamine.de.
Proximity-dependent biotin labelling reveals CP190 as an EcR/Usp molecular partner
Authors: Mazina Marina Yu. , Ziganshin Rustam H. , Magnitov Mikhail D. , Golovnin Anton K. , Vorobyeva Nadezhda E.
Published: March 2020

Proximity-dependent biotin labelling revealed undescribed participants of the ecdysone response in Drosophila. Two labelling enzymes (BioID2 and APEX2) were fused to EcR or Usp to biotin label the surrounding proteins. The EcR/Usp heterodimer was found to collaborate with nuclear pore subunits, chromatin remodelers, and architectural proteins. Many proteins identified through proximity-dependent labelling with EcR/Usp were described previously as functional components of an ecdysone response, corroborating the potency of this labelling method. A link to ecdysone response was confirmed for some newly discovered regulators by immunoprecipitation of prepupal nuclear extract with anti-EcR antibodies and functional experiments in Drosophila S2 cells. A more in-depth study was conducted to clarify the association of EcR/Usp with one of the detected proteins, CP190, a well-described cofactor of Drosophila insulators. CP190 was found to co-immunoprecipitate with the EcR subunit of EcR/Usp in a 20E-independent manner. ChIP-Seq experiments revealed only partial overlapping between CP190 and EcR bound sites in the Drosophila genome and complete absence of CP190 binding at 20E-dependent enhancers. Analysis of Hi-C data demonstrated an existence of remote interactions between 20E-dependent enhancers and CP190 sites which suggests formation of a protein complex between EcR/Usp and CP190 through the space. Our results support the previous concept that CP190 has a role in stabilization of specific chromatin loops for proper activation of transcription of genes regulated by 20E hormone.
Seeking high-priority mutations enabling successful antibody-breeding: systematic analysis of a mutant that gained over 100-fold enhanced affinity
Authors: Oyama Hiroyuki , Kiguchi Yuki , Morita Izumi , Yamamoto Chika , Higashi Yuka , Taguchi Miku , Tagawa Tatsuya , Enami Yuri , Takamine Yuriko , Hasegawa Hanako , Takeuchi Atsuko , Kobayashi Norihiro
Published: March 2020

“Antibody-breeding” has provided therapeutic/diagnostic antibody mutants with greater performance than native antibodies. Typically, random point mutations are introduced into the VH and VL domains of parent antibodies to generate diverse libraries of single-chain Fv fragments (scFvs), from which evolved mutants are selected. We produced an scFv against estradiol-17β with 11 amino acid substitutions and a >100-fold improved affinity constant (Ka = 1.19 × 1010 M−1) over the parent scFv, enabling immunoassays with >30-fold higher sensitivity. We systematically analyzed contributions of these substitutions to the affinity enhancement. Comparing various partial scFv revertants based on their Kas indicated that a revertant with four substitutions (VH-L100gQ, VL-I29V, -L36M, -S77G) exhibited somewhat higher affinity (Ka = 1.46 × 1010 M−1). Finally, the VH-L100gQ substitution, occurring in VH complementarity-determining region (CDR) 3, was found to be the highest-priority for improving the affinity, and VL-I29V and/or VL-L36M cooperated significantly. These findings encouraged us to reconsider the potential of VH-CDR3-targeting mutagenesis, which has been frequently attempted. The substitution(s) wherein might enable a “high rate of return” in terms of selecting mutants with dramatically enhanced affinities. The “high risk” of generating a tremendous excess of “junk mutants” can be overcome with the efficient selection systems that we developed.
Hierarchical Eutectoid Nano-lamellar Decomposition in an Al0.3CoFeNi Complex Concentrated Alloy
Authors: Dasari Sriswaroop , Gwalani Bharat , Jagetia Abhinav , Soni Vishal , Gorsse Stéphane , Banerjee Rajarshi
Published: March 2020

This paper reports a novel eutectoid nano-lamellar (FCC + L12)/(BCC + B2) microstructure that has been discovered in a relatively simple Al0.3CoFeNi high entropy alloy (HEA) or complex concentrated alloy (CCA). This novel eutectoid nano-lamellar microstructure presumably results from the complex interplay between Al-mediated lattice distortion (due to its larger atomic radius) in a face-centered cubic (FCC) CoFeNi solid solution, and a chemical ordering tendency leading to precipitation of ordered phases such as L12 and B2. This eutectoid microstructure is a result of solid-state decomposition of the FCC matrix and therefore distinct from the commonly reported eutectic microstructure in HEAs which results from solidification. This novel nano-lamellar microstructure exhibits a tensile yield strength of 1074 MPa with a reasonable ductility of 8%. The same alloy can be tuned to form a more damage-tolerant FCC + B2 microstructure, retaining high tensile yield stress (~900 MPa) with appreciable tensile ductility (>20%), via annealing at 700 °C. Such tunability of microstructures with dramatically different mechanical properties can be effectively engineered in the same CCA, by exploiting the complex interplay between ordering tendencies and lattice distortion.
Impact of projected sea surface temperature biases on tropical cyclones projections in the South Pacific
Authors: Dutheil C. , Lengaigne M. , Bador M. , Vialard J. , Lefèvre J. , Jourdain N. C. , Jullien S. , Peltier A. , Sultan B. , Menkès C.
Published: March 2020

Climate model projections generally indicate fewer but more intense tropical cyclones (TCs) in response to increasing anthropogenic emissions. However these simulations suffer from long-standing biases in their Sea Surface Temperature (SST). While most studies investigating future changes in TC activity using high-resolution atmospheric models correct for the present-day SST bias, they do not consider the reliability of the projected SST changes from global climate models. The present study illustrates that future South Pacific TC activity changes are strongly sensitive to correcting the projected SST changes using an emergent constraint method. This additional correction indeed leads to a strong reduction of the cyclogenesis (−55%) over the South Pacific basin, while no statistically significant change arises in the uncorrected simulations. Cyclogenesis indices suggest that this strong reduction in the corrected experiment is caused by stronger vertical wind shear in response to a South Pacific Convergence Zone equatorward shift. We thus find that uncertainty in the projected SST patterns could strongly hamper the reliability of South Pacific TC projections. The strong sensitivity found in the current study will need to be investigated with other models, observational constraint methods and in other TC basins in order to assess the reliability of regional TC projections.
Synergistic enhancement in the microelectronic properties of poly-(dioctylfluorene) based Schottky devices by CdSe quantum dots
Authors: Muhammad Fida , Tahir Muhammad , Zeb Muhammad , Kalasad Muttanagoud N. , Mohd Said Suhana , Sarker Mahidur R. , Sabri Mohd Faizul Mohd , Ali Sawal Hamid Md
Published: March 2020

This paper reports the potential application of cadmium selenide (CdSe) quantum dots (QDs) in improving the microelectronic characteristics of Schottky barrier diode (SBD) prepared from a semiconducting material poly-(9,9-dioctylfluorene) (F8). Two SBDs, Ag/F8/P3HT/ITO and Ag/F8-CdSe QDs/P3HT/ITO, are fabricated by spin coating a 10 wt% solution of F8 in chloroform and 10:1 wt% solution of F8:CdSe QDs, respectively, on a pre-deposited poly(3-hexylthiophene) (P3HT) on indium tin oxide (ITO) substrate. To study the electronic properties of the fabricated devices, current-voltage (I–V) measurements are carried out at 25 °C in dark conditions. The I–V curves of Ag/F8/P3HT/ITO and Ag/F8-CdSe QDs/P3HT/ITO SBDs demonstrate asymmetrical behavior with forward bias current rectification ratio (RR) of 7.42 ± 0.02 and 142 ± 0.02, respectively, at ± 3.5 V which confirm the formation of depletion region. Other key parameters which govern microelectronic properties of the fabricated devices such as charge carrier mobility (µ), barrier height (ϕb), series resistance (Rs) and quality factor (n) are extracted from their corresponding I–V characteristics. Norde’s and Cheung functions are also applied to characterize the devices to study consistency in various parameters. Significant improvement is found in the values of Rs, n, and RR by 3, 1.7, and 19 times, respectively, for Ag/F8-CdSe QDs/P3HT/ITO SBD as compared to Ag/F8/P3HT/ITO. This enhancement is due to the incorporation of CdSe QDs having 3-dimensional quantum confinement and large surface-to-volume area. Poole-Frenkle and Richardson-Schottky conduction mechanisms are also discussed for both of the devices. Morphology, optical bandgap (1.88 ± 0.5 eV) and photoluminescence (PL) spectrum of CdSe QDs with a peak intensity at 556 nm are also reported and discussed.
Analysis of L-arginine:glycine amidinotransferase-, creatine- and homoarginine-dependent gene regulation in the murine heart
Authors: Jensen Märit , Müller Christian , Choe Chi-un , Schwedhelm Edzard , Zeller Tanja
Published: March 2020

L-arginine:glycine amidinotransferase (AGAT) and its metabolites creatine and homoarginine (HA) have been linked to cardiovascular pathologies in both human and murine studies, but the underlying molecular mechanisms are poorly understood. Here, we report the first analysis of heart transcriptome variation using microarrays in an AGAT-deficient (AGAT−/−) mouse model to evaluate AGAT-, creatine- and HA-dependent gene regulation. Our data revealed significant differences of gene expression between AGAT−/− and wild-type (WT) mice, affecting cardiac energy metabolism (Fbp2, Ucp2), cardiac hypertrophy and fibrosis (Nppa, Ctgf), immune response (Fgl2), and the conduction system of the heart (Dsc2, Ehd4, Hcn2, Hcn4, Scn4a, Scn4b). All of these genes being expressed on WT level in creatine-supplemented mice. Using in silico analysis based on the GEO database we found that most of these candidate genes (Ctgf, Dsc2, Fbp2, Fgl2, Hcn2, Nppa)  revealed significant alterations in a WT mouse model of myocardial infarction underlining a pathophysiological relationship between AGAT metabolism and cardiovascular disease.
Weak Antilocalization and Anisotropic Magnetoresistance as a Probe of Surface States in Topological Bi2TexSe3−x Thin Films
Authors: Stephen Gregory M. , Vail Owen. A. , Lu Jiwei , Beck William A. , Taylor Patrick J. , Friedman Adam L.
Published: March 2020

Topological materials, such as the quintessential topological insulators in the Bi2X3 family (X = O, S, Se, Te), are extremely promising for beyond Moore’s Law computing applications where alternative state variables and energy efficiency are prized. It is essential to understand how the topological nature of these materials changes with growth conditions and, more specifically, chalcogen content. In this study, we investigate the evolution of the magnetoresistance of Bi2TexSe3−x for varying chalcogen ratios and constant growth conditions as a function of both temperature and angle of applied field. The contribution of 2D and 3D weak antilocalization are investigated by utilizing the Tkachov-Hankiewicz model and Hakami-Larkin-Nagaoka models of magnetoconductance.
Retrospective Motion Correction in Multishot MRI using Generative Adversarial Network
Authors: Usman Muhammad , Latif Siddique , Asim Muhammad , Lee Byoung-Dai , Qadir Junaid
Published: March 2020

Multishot Magnetic Resonance Imaging (MRI) is a promising data acquisition technique that can produce a high-resolution image with relatively less data acquisition time than the standard spin echo. The downside of multishot MRI is that it is very sensitive to subject motion and even small levels of motion during the scan can produce artifacts in the final magnetic resonance (MR) image, which may result in a misdiagnosis. Numerous efforts have focused on addressing this issue; however, all of these proposals are limited in terms of how much motion they can correct and require excessive computational time. In this paper, we propose a novel generative adversarial network (GAN)-based conjugate gradient SENSE (CG-SENSE) reconstruction framework for motion correction in multishot MRI. First CG-SENSE reconstruction is employed to reconstruct an image from the motion-corrupted k-space data and then the GAN-based proposed framework is applied to correct the motion artifacts. The proposed method has been rigorously evaluated on synthetically corrupted data on varying degrees of motion, numbers of shots, and encoding trajectories. Our analyses (both quantitative as well as qualitative/visual analysis) establish that the proposed method is robust and reduces several-fold the computational time reported by the current state-of-the-art technique.
Antioxidant evaluation-guided chemical profiling and structure-activity analysis of leaf extracts from five trees in Broussonetia and Morus (Moraceae)
Authors: Cao Xinxin , Yang Lingguang , Xue Qiang , Yao Fan , Sun Jing , Yang Fuyu , Liu Yujun
Published: March 2020

Morus and Broussonetia trees are widely used as food and/or feed. Among 23 phenolics identified from leaves of five Moraceae species using UPLC–QTOF–MS/MS, 15 were screened using DPPH/ABTS-guided HPLCs, including seven weak (flavonoids with one hydroxyl on B-ring) and eight strong (four caffeoylquinic acids and four flavonoids, each with a double hydroxyl on B-ring) antioxidants. We then determined the activity and synergistic effects of individual antioxidants and a mixture of the eight strongest antioxidants using DPPH-guided HPLC. Our findings revealed that (1) flavonoid glucuronide may have a more negative effect on antioxidant activity than glucoside, and (2) other compounds in the mixture may exert a negative synergistic effect on antioxidant activity of the four flavonoids with B-ring double hydroxyls but not the four caffeoylquinic acids. In conclusion, the eight phenolics with the strongest antioxidant ability reliably represented the bioactivity of the five extracts examined in this study. Moreover, the Morus alba hybrid had more phenolic biosynthesis machinery than its cross-parent M. alba, whereas the Broussonetia papyrifera hybrid had significantly less phenolic machinery than B. papyrifera. This difference is probably the main reason for livestock preference for the hybrid of B. papyrifera over B. papyrifera in feed.
Molecular classification of breast cancer using the mRNA expression profiles of immune-related genes
Authors: Mei Juan , Zhao Ji , Fu Yi
Published: March 2020

Breast cancer is the most lethal cancer in women and displaying a broad range of heterogeneity in terms of clinical, molecular behavior and response to therapy. Increasing evidence demonstrated that immune-related genes were an important source of prognostic information for several types of tumors. In this study, the k-mean clustering was applied to gene expression data from the immune-related genes, two molecular clusters were identified for 1980 breast cancer patients. The prognostic significance of the immune-related genes based classification was confirmed in the log-rank test. These clusters were also associated with immune checkpoints, immune-related features and tumor infiltrating levels. In addition, we used the shrunken centroid algorithm to predict the cluster of a given breast cancer sample, and good predictive results were obtained by this algorithm. These results indicated that the proposed classification method is a promising method, and we hope that this method may improve the treatment stratification of breast cancer in the future.