bluuebunny/crossref_metadata_2025 huggingface dataset:
Surface Modification of Commercial Cotton Yarn as Electrode for Construction of Flexible Fiber-Shaped Supercapacitor
Authors: Xiao Wei , Huang Jing , Zhou Wenjie , Jiang Qinglin , Deng Ying , Zhang Yanhua , Tian Liangliang
Published: September 2021

In this study, we report on the rational design and facile preparation of a cotton-reduced graphene oxide-silver nanoparticle (cotton-RGO-AgNP) hybrid fiber as an electrode for the building of a flexible fiber-shaped supercapacitor (FSSC). It was adequately characterized and found to possess a well-defined core−shell structure with cotton yarn as a core and a porous RGO-AgNP coating as a shell. Thanks to the unique morphological features and low electrical resistance (only 2.3 Ω·cm−1), it displayed attractive supercapacitive properties. When evaluated in a three-electrode setup, this FSSC electrode delivered the highest linear and volumetric specific capacitance of up to ca. 12.09 mF·cm−1 and ca. 9.67 F·cm−3 with a satisfactory rate capability as well as a decent cycling stability. On the other hand, an individual parallel symmetric FSSC cell constructed by this composite fiber fulfilled the largest linear and volumetric specific capacitance of ca. 1.67 mF·cm−1 and ca. 0.67 F·cm−3 and offered the maximum energy density, as high as ca. 93.1 μWh·cm−3, which outperformed a great number of graphene- and textile yarn-based FSSCs. Impressively, bending deformation brought about quite a limited effect on its electrochemical behaviors and almost no capacitance degradation took place during the consecutive charge/discharge test for over 10,000 cycles. Consequently, these remarkable performances suggest that the currently developed cotton-RGO-AgNP fiber has considerable application potential in flexible, portable and wearable electronics.
Identification of Colourants and Varnishes in a 14th Century Decorated Wood-Carved Door of the Dionysiou Monastery in Mount Athos
Authors: Konstantas Alexander , Karapanagiotis Ioannis , Boyatzis Stamatis C.
Published: September 2021

A decorated and carved wooden door of the late Byzantine period (14th Century), which belongs to the Dionysiou Monastery in Mount Athos, Greece, constitutes an important relic of valuable technological information due to its construction technology and history. Seventeen (17) samples detached from the door are studied using optical microscopy, scanning electron microscopy (SEM) with energy dispersive X-ray analysis (SEM-EDX), and micro-Raman and FTIR spectroscopy. The following materials are identified in the cross sections of the door samples using micro-Raman spectroscopy: orpiment, lead white, red lead, red ochre, cinnabar, carbon black, gypsum, anhydrite, and calcite, and an organic colourant of the indigoid family. SEM-EDX studies supported to the aforementioned Raman results. Interestingly, a combination of inorganic and organic colourants was detected. The main goals of this particular study were to: (a) reveal the colour palette and materials, (b) identify the type of varnish and its condition, and (c) contribute to future restoration processes and aid conservators in selecting compatible restoration materials.
Development of Chitosan/Squid Skin Gelatin Hydrolysate Films: Structural, Physical, Antioxidant, and Antifungal Properties
Authors: Cuevas-Acuña Dulce Alondra , Plascencia-Jatomea Maribel , Santacruz-Ortega Hisila del Carmen , Torres-Arreola Wilfrido , Ezquerra-Brauer Josafat Marina
Published: September 2021

Chitosan (85% deacetylated, viscosity > 400 MPa, and molecular weight of 570.3 kDa)/squid gelatin hydrolysates (SGH) were prepared by incorporating SGHs (10%, 20%, and 40%) into chitosan films. SGH were obtained from squid skin gelatin by hydrolysis with Alcalase. The effects of adding SGH on the physical, chemical structure, mechanical, degradability, antioxidant, and antifungal properties of the chitosan films were evaluated. Films containing SGH were opaquer and more colored than the reference. Scanning electron microscope imaging showed that the surface sections of the CH/SGH films were smooth and homogeneous, though a small amount of insoluble microparticles was observed. Atomic force microscopy indicated that the surface roughness of the chitosan films increased with the addition of SGH. Fourier-transform infrared spectroscopy and nuclear magnetic resonance spectroscopy suggested an excellent compatibility of the components due to hydrogen bonding. The flexibility and in vitro degradability of the films increased as the SGH content increased. The 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate acid and 1,1-diphenyl-2-picrylhydrazyl scavenging rate of films increased with the addition of SGH. Moreover, films containing 20% SGH improved the fungistatic activity against Aspergillus parasiticus of chitosan films. The chitosan/SGH composite films have the potential for use in food packaging.
Analysis of Heat Transfer Characteristics of a Heat Exchanger Based on a Lattice Filling
Authors: Lai Xuhui , Wang Caihua , Peng Dongjian , Yang Huanqing , Wei Zhengying
Published: September 2021

In response to the heat load requirements of the high-thrust liquid rocket engine, a light-weight lattice structure is used to fill traditional a heat exchanger. A parameterized model library of the lattice structure is established, and the relative density of the lattice structure is adjusted by changing the unit cell structure parameters to obtain different filling structures. A comprehensive comparison of heat exchangers with different filling structures performed in terms of weight, heat transfer efficiency, and turbulence intensity. Using the finite difference method, the numerical calculation of the non-steady heat–fluid–solid coupling conjugate heat transfer of the eight-lattice structure is performed, and the dynamic heat transfer process between the lattice structure and liquid oxygen is simulated using the VOF model and the SST k-ω model. The results show that the pressure of the fluid in the heat exchanger increases with increasing relative density, leading to a high outlet temperature and greatly increasing the outlet velocity. The support trusses close to the wall obviously hinder the flow of liquid oxygen, resulting in a sudden change in the flow rate behind the support trusses, driving the high-temperature fluid at the bottom to move upwards. The direction of the support trusses and the unit cell porosity have a greater impact on the liquid oxygen flow rate, which in turn affects the flow and heat transfer performance of the heat exchanger. In consideration of the heat load requirements of the heat exchanger, star-type lattices are used to fill the heat exchanger. When the flow is fully developed, the volume ratio of the heated fluid is 85.60%, and the outlet temperature is 390 K, which meets the design requirements.
Perceptions about the Future of Integrating Emerging Technologies into Higher Education—The Case of Robotics with Artificial Intelligence
Authors: Leoste Janika , Jõgi Larissa , Õun Tiia , Pastor Luis , San Martín López José , Grauberg Indrek
Published: September 2021

Emerging technologies (ETs) will most likely have a strong impact on education (starting with higher education), just like they have already had in so many economic and social areas. This paper is based on the results obtained in the project “My Future Colleague Robot”, an initiative that aimed to improve the competence of university teaching staff regarding the introduction of ETs in teaching practices at university level. In this paper, we identified the strengths and weaknesses, opportunities, and threats that are related to the adoption in higher education of the combination of two ETs: robotics together with artificial intelligence (AI). Additionally, we analyzed the perceptions of university-level teaching staff about the potential of introducing ETs in education. The empirical data presented here were collected using written essays from 18 university teachers and students. Deductive and inductive approaches with thematic analysis were used for the data analysis. The findings support the idea that previous ET-related experience can support positive attitudes and the implementations of ETs in university teaching; in this study, university teachers had optimistic expectations towards ETs, accepting them as part of teaching practice development, while discussion about the negative effects of ETs was negligible.
In Situ Evolution of Pores in Lithium Hydride at Elevated Temperatures Characterized by X-ray Computed Tomography
Authors: Shi Yifan , Peng Lei , Zhang Wangzi , Li Qiang , Li Qishou , Ye Linsen
Published: September 2021

The evolution of defects such as pores at elevated temperatures is crucial for revealing the thermal stability of lithium hydride ceramic. The in situ evolution of pores in sintered lithium hydride ceramic from 25 °C to 500 °C, such as the statistics of pores and the 3D structure of pores, was investigated by X-ray computed tomography. Based on the statistics of pores, the porosity significantly increased from 25 °C to 200 °C and decreased after 200 °C, due to the significant change in the number and total volume of the round-shaped pores and the branched crack-like pores with an increasing temperature. According to the 3D structure of pores, the positions of pores did not change, and the sizes of pores went up in the range of 25–200 °C and went down after 200 °C. Some small round-shaped pores with an Equivalent Diameter of less than 9 μm appeared at 200 °C and disappeared at elevated temperatures. Some adjacent pores of all types connected at 200 °C, and some branched crack-like pores gradually disconnected with an increasing temperature. The expansion of pores at 200 °C caused by the release of residual hydrogen and the contraction of pores after 200 °C because of the migration and diffusion of some hydrogen in pores might be the reason for the evolution of pores with an increasing temperature.
The Morphology, Ultrastructure and Molecular Phylogeny of a New Freshwater Heterolobose Amoeba Parafumarolamoeba stagnalis n. sp. (Vahlkampfiidae; Heterolobosea)
Authors: Borodina Anastasia , Mylnikov Alexander , Janouškovec Jan , Keeling Patrick , Tikhonenkov Denis
Published: September 2021

Heterolobose amoebae are important members of marine, freshwater, and soil microbial communities, but their diversity remains under-explored. We studied the diversity of Vahlkampfiidae to improve our understanding of heterolobosean relationships and their representation in aquatic benthos. Using light and electron microscopy, and molecular phylogenies based on the SSU rRNA and ITS loci, we describe the fine morphology and evolutionary relationships of a new heterolobosean Parafumarolamoeba stagnalis n. sp. from a small pond in European Russia. Cells of P. stagnalis possess a clearly distinguishable anterior hyaline pseudopodium, eruptive movement, several thin and sometimes branched uroidal filaments, spherical cysts without pores and plugs, and mitochondria that have discoid cristae and are surrounded by cisternae of the endoplasmic reticulum. The genus Parafumarolamoeba has so far included a single species, Parafumarolamoeba alta from high-altitude soil in Tibet, which is morphologically distinct from P. stagnalis. Taxonomic description for a new Parafumarolamoeba species is therefore provided.
EUS-Guided Biopsy with a Novel Puncture Biopsy Forceps Needle—Feasibility Study
Authors: Litjens Geke , Gerges Christian , Shastri Yogesh M. , Somani Piyush , Beyna Torsten , Neuhaus Horst , van Laarhoven Cornelis J. H. M. , Prokop Mathias , Siersema Peter D. , Hermans John J. , van Geenen Erwin J. M.
Published: September 2021

Endoscopic ultrasound (EUS) with fine needle aspiration (FNA) or biopsy (FNB) to diagnose lesions in the gastrointestinal tract is common. Demand for histology sampling to identify treatment-specific targets is increasing. Various core biopsy FNB needles to obtain tissue for histology are currently available, however, with variable (37–97%) histology yields. In this multicenter study, we evaluated performance, safety, and user experience of a novel device (the puncture biopsy forceps (PBF) needle). Twenty-four procedures with the PBF needle were performed in 24 patients with a suspected pancreatic lesion (n = 10), subepithelial lesion (n = 10), lymph node (n = 3), or pararectal mass (n = 1). In 20/24 (83%) procedures, the PBF needle yielded sufficient material for interpretation (sample adequacy). In 17/24 (71%), a correct diagnosis was made with the material from the PBF needle (diagnostic accuracy). All participating endoscopists experienced a learning curve. (Per)procedural technical issues occurred in four cases (17%), but there were no adverse events. The PBF needle is a safe and potentially useful device to obtain an EUS-guided biopsy specimen. As the design of the PBF needle is different to core biopsy FNB needles, specific training will likely further improve the performance of the PBF needle. Furthermore, the design of the needle needs further improvement to make it more robust in clinical practice.
Simultaneously Monitoring Whole Corneal Injury with Corneal Optical Density and Thickness in Patients Undergoing Cataract Surgery
Authors: Hsieh Tzu-Han , Yu Hun-Ju , Yang I-Hui , Ho Ren-Wen , Hsiao Yu-Ting , Fang Po-Chiung , Kuo Ming-Tse
Published: September 2021

To pursue the least corneal implication during cataract surgery, this study aimed to monitor corneal wound injury after cataract surgery with a novel method. The prospective cohort study involved thirty-two patients, who were assessed by a Scheimpflug tomography AxL® (Oculus GmbH, Wetzlar, Germany) via the following two kinds of indices: whole corneal optical density (COD) and corneal thickness (CT), two weeks before and one month after cataract surgery. The results of the COD revealed that corneal annuli 0.0–2.0 mm and 2.0–6.0 mm, and the average and maximal values at the incisional site significantly increased postoperatively. Also, the anterior and central stroma of 0.0–2.0 mm, and all three depths of 2.0–6.0 mm, increased remarkably after the operation. For the CT, all ranges of diameters plus incisional sites showed significant increases postoperatively. Furthermore, we analyzed the differences (delta) of COD and CT between pre- and post-operation, and found significant correlations between the delta of COD and the delta of CT, regarding annuli 0.0–2.0 mm, 2.0–6.0 mm, and 6.0–10.0 mm, but no correlation at the incisional site, with either average density or maximal density, was detected. We concluded that whole COD and CT, especially at the central zones of the cornea (annulus < 6 mm), are both valuable parameters in the assessment of corneal damage post-cataract surgery, and are independent indices at the incisional site.
Laser Doppler Imaging for Treating Vascular Complications from Procedures Involving Dermal Fillers: Case Series and Literature Review
Authors: Lee An-Li , Chen Yu-Fan , Yao Wen-Teng , Liu Ying-Chun , Yu Chia-Meng , Yu Chieh-Ming , Tu Chih-Peng , Huang Wen-Chen , Tung Kwang-Yi , Tsai Ming-Feng
Published: September 2021

Vascular occlusion is a rare but severe complication of dermal filler injections. Early treatment of this complication produces better outcomes. Current diagnostic methods for vascular occlusion in the skin are subjective and imprecise; these include capillary refill time, skin color, and reports of pain. This study aimed to assess the use of laser Doppler imaging (LDI) in the evaluation and treatment of vascular complications caused by dermal filler injections. This retrospective study used laser Doppler imaging (LDI) in 13 patients who developed vascular occlusion after facial dermal filler injections, with subsequent follow-up. The precise areas of perfusion observed on LDI were compared with the findings of clinical and photographic evaluation. The results showed that LDI accurately identified areas of vascular occlusion and improved treatment precision among these thirteen patients. The procedure was more precise than visual inspection or photographic evidence. Satisfactory outcomes were achieved for all patients, and no procedure-related complications were reported. Collectively, LDI provides fast, noninvasive, and accurate delineation of areas of vascular occlusion caused by complications of dermal filler injections and avoids several subjective shortcomings of visual and photographic evaluations. Thus, LDI effectively tracks treatment outcomes. However, large-scale studies are required to confirm the present findings.
Effectiveness and Safety of Transthoracic Ultrasound in Guiding Percutaneous Needle Biopsy in the Lung and Comparison vs. CT Scan in Assessing Morphology of Subpleural Consolidations
Authors: Sperandeo Marco , Maiello Evaristo , Graziano Paolo , Simeone Annalisa , De Cosmo Salvatore , Dimitri Lucia , Di Micco Concetta , Perrone Elio , Taurchini Marco , Ferretti Gianmaria , Mirijello Antonio , Varriale Antonio , Grimaldi Maria , Lacedonia Donato , Quarato Carla
Published: September 2021

(1) Background: The aim of this study was to conduct a prospective analysis on the diagnostic accuracy of transthoracic ultrasound-guided percutaneous needle biopsy (TUS-PNB) for the histological assessment of peripheral lung lesions and to assess the performance of transthoracic ultrasound (TUS) examination vs. chest CT (gold standard) in the differentiation between malignant and benign peripheral lung lesions. (2) Methods: A total of 961 consecutive patients with subpleural pulmonary lesions were enrolled. All the patients received a CT scan with contrast; 762 patients underwent TUS-PTNB for suspicion of malignancy, and the remaining 199 enrolled patients underwent only TUS examination as a part of routine follow-up for known non-malignant subpleural consolidations. (3) Results: Among the 762 TUS-guided biopsies, there were 627 (82.28%) malignant lesions, 82 (10.76%) benign lesions, and 53 (6.96%) indeterminate lesions. The overall diagnostic accuracy was 93.04%. The rates of pneumothorax not requiring chest-tube insertion and self-limited hemoptysis were 0.79 and 0.26%, respectively. Patients were divided into two groups based on the benign or malignant nature of the subpleural consolidations. On TUS, both malignant and benign lesions showed mostly irregular margins and a hypoechoic pattern, but no differences were assessed in terms of sonographic margins and pattern between the two groups. There was poor agreement between TUS and chest CT in assessing air bronchograms and necrotic areas. The only finding in the detection of which TUS showed superiority compared to chest-CT was pleural effusion. (4) Conclusions: TUS-PNB was confirmed to be an effective and safe diagnostic method for peripheral pulmonary consolidation, but their sonographic pattern did not allow to rule out a malignant nature. A pre-operative evaluation on CT images, combined with the possibility of performing additional immunohistochemical and cytological investigations and the experience of the medical staff, may improve the diagnostic yield of TUS-guided biopsies.
The Potential of Lung Epithelium Specific Proteins as Biomarkers for COVID-19-Associated Lung Injury
Authors: Almuntashiri Sultan , James Chelsea , Wang Xiaoyun , Siddiqui Budder , Zhang Duo
Published: September 2021

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection was first reported in Wuhan, China, and was declared a pandemic by the World Health Organization (WHO) on 20 March 2020. The respiratory system is the major organ system affected by COVID-19. Numerous studies have found lung abnormalities in patients with COVID-19, including shortness of breath, respiratory failure, and acute respiratory distress syndrome. The identification of lung-specific biomarkers that are easily measurable in serum would be valuable for both clinicians and patients with such conditions. This review is focused on the pneumoproteins and their potential to serve as biomarkers for COVID-19-associated lung injury, including Krebs von den Lungen-6 (KL-6), surfactant proteins (SP-A, SP-B, SP-C, SP-D), and Clara cell secretory protein (CC16). The current findings indicate the aforementioned pneumoproteins may reflect the severity of pulmonary manifestations and could serve as potential biomarkers in COVID-19-related lung injury.
Coexistence of Primary Myelofibrosis and Paroxysmal Nocturnal Hemoglobinuria Clone with JAK2 V617F, U2AF1 and SETBP1 Mutations: A Case Report and Brief Review of Literature
Authors: Park Sholhui , So Min-Kyung , Cho Min-Sun , Kim Dae-Young , Huh Jungwon
Published: September 2021

Primary myelofibrosis (PMF) and paroxysmal nocturnal hemoglobinuria (PNH) are very rare diseases, respectively, and it is uncommon to have both diseases together. Mutational profiling using next-generation sequencing in PMF and PNH detected additional mutations associated with myeloid neoplasms, suggesting a step-wise clonal evolution. We present here a very rare case with PMF and PNH with JAK2 V617F, U2AF1 and SETBP1 mutations at the time of diagnosis. The combination of these two diseases and three genetic mutations is difficult to interpret at once. (i.e., the sequence of these two clonal diseases or the time points of acquiring these mutations). Our report suggests that when diagnosing or treating patients with PMF, it is necessary to keep in mind that PNH may be present at the same time or sometimes new. The genetic mutations simultaneously found in this patient require further research to elucidate the clinical significance and their genetic associations fully.
RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection Methods
Authors: Selvam Kasturi , Najib Mohamad , Khalid Muhammad , Mohamad Suharni , Palaz Fahreddin , Ozsoz Mehmet , Aziah Ismail
Published: September 2021

Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has attracted public attention. The gold standard for diagnosing COVID-19 is reverse transcription–quantitative polymerase chain reaction (RT-qPCR). However, RT-qPCR can only be performed in centralized laboratories due to the requirement for advanced laboratory equipment and qualified workers. In the last decade, clustered regularly interspaced short palindromic repeats (CRISPR) technology has shown considerable promise in the development of rapid, highly sensitive, and specific molecular diagnostic methods that do not require complicated instrumentation. During the current COVID-19 pandemic, there has been growing interest in using CRISPR-based diagnostic techniques to develop rapid and accurate assays for detecting SARS-CoV-2. In this work, we review and summarize reverse-transcription loop-mediated isothermal amplification (RT-LAMP) CRISPR-based diagnostic techniques for detecting SARS-CoV-2.
Students’ Perceptions of Doctoral Defense Formats
Authors: Lantsoght Eva O. L.
Published: September 2021

The doctoral defense is an important step in the doctoral journey and an essential requirement for obtaining a doctoral degree. Past research on the doctoral defense has focused solely on national practices. In this work, I investigate the potential link between the doctoral defense format based on its major and minor elements and the perception of the defense by the student. For this purpose, I first reviewed the different defense formats used internationally to extract the different elements of the doctoral defense, and the literature on students’ perceptions of the doctoral defense. Then, I carried out an international survey which received 297 responses, of which 204 were completed surveys which I used for the analysis in this article. I first analyzed the outcomes of the survey using qualitative and quantitative methods, and then cross-correlated the outcomes of defense format with the outcomes of student perception. From this analysis, I observed that the defense elements that positively impact the student’s perception are: publication of the thesis before the defense, receiving committee feedback before the defense, knowing the recommendations of one or more committee member in advance, having the supervisor present in the audience or as part of the committee, using a dress code, and including a laudatio. The final conclusion of this work is threefold. The first conclusion is that the details of the defense format impact most the students’ perception. The second conclusion is that doctoral students, on average, value the defense as a positive experience. The third conclusion is that the defense format cannot influence two important aspects of how a student perceives the defense: the student’s inner life and experience during the defense, and the behavior of the committee members.
Perception of Risk in the Use of Technologies and Social Media. Implications for Identity Building during Adolescence
Authors: Torrijos-Fincias Patricia , Serrate-González Sara , Martín-Lucas Judith , Muñoz-Rodríguez José Manuel
Published: September 2021

Life for today’s adolescents generally involves spending a large part of their time in front of a screen, permanently connected to the internet. It is no surprise to learn that recent years have witnessed an increase in the number of studies on how adolescent identities are being affected by the phenomenon of hyperconnectivity. This article addresses the perception adolescents have of the uses and functions they encounter during the time they are online and their self-perceived risks, as well as the tools or strategies they use to tackle the threats of a hyperconnected society. This involved designing a qualitative study in which 130 adolescents took part in different focus groups. The results revealed that adolescents use technologies mainly as a means of communication and entertainment, and as they mature, they perceive greater risks associated with this use. The study also found that they deploy few tools and strategies to deal with the self-perceived risks.
A Critical Review of Mobile Learning: Phoenix, Fossil, Zombie or …..?
Authors: Traxler John
Published: September 2021

The established mobile learning paradigm is now two decades old; it grew out of the visions and resources of e-learning research communities in universities in the world’s more economically developed regions. Whilst it has clearly been able to demonstrate many practical, pedagogic and conceptual achievements, it is now running out of steam. It has failed to adapt to a world where mobile technologies are pervasive, ubiquitous and intrusive and where people and communities can now own their own learning. This paper looks at the evolution of the established mobile learning paradigm and explores the current global, demographic, social and technical environment in order to develop a new paradigm more suited to the changed and changing realities and priorities. This is mobile learning2.0. The paper looks at the axioms and values of this paradigm and its possible tools and techniques. The treatment is discursive and critical. The paper reimagines the concepts and practices of learning with mobiles. It embraces many significant themes at a high level, including inclusive and equitable education; learning theories and design; pedagogical frameworks and methodologies; digital and media literacies; social media and learning environments; online collaboration and communities; Informal and formal learning.
A Survey of Wireless Battery Management System: Topology, Emerging Trends, and Challenges
Authors: Samanta Akash , Williamson Sheldon S.
Published: September 2021

An effective battery management system (BMS) is indispensable for any lithium-ion battery (LIB) powered systems such as electric vehicles (EVs) and stationary grid-tied energy storage systems. Massive wire harness, scalability issue, physical failure of wiring, and high implementation cost and weight are some of the major issues in conventional wired-BMS. One of the promising solutions researchers have come up with is the wireless BMS (WBMS) architecture. Despite research and development on WBMS getting momentum more than a decade ago, it is still in a preliminary stage. Significant further upgradation is required towards developing an industry-ready WBMS, especially for high-power LIB packs. Therefore, an in-depth survey exclusively on WBMS architectures is presented in this article. The aim is to provide a summary of the existing developments as well as to present an informative guide to the research community for future developments by highlighting the issues, emerging trends, and challenges. In-depth analysis of the existing WBMS topologies will not only help the researchers to understand the existing challenges and future research scopes clearly but at the same time enthuse them to focus their research inclination in the domain of WBMS.
The Effect of Detoxification of Lignocellulosic Biomass for Enhanced Methane Production
Authors: Kotarska Katarzyna , Dziemianowicz Wojciech , Świerczyńska Anna
Published: September 2021

The aim of this research is to examine the effect of lignocellulosic biomass detoxification on the efficiency of the methane fermentation process. Both for corn straw and rye straw, the methane yield was expressed per volume of fermentation medium and per mass of volatile solids (VS) added. Lignocellulosic biomass was subjected of thermo-chemical and enzymatic sequential pretreatments. It was found that methane yield was higher by 22% when using the detoxification process. In these variants, CH4 yield was 18.86 L/L for corn straw and 17.69 L/L for rye straw; while methane yield expressed per mass of VS added was 0.31 m3/kg VS for corn straw and 0.29 m3/kg VS for rye straw. The inclusion of a detoxification step in pretreatments of biomass lignocellulosic increases the degree of organic substance decomposition and enhances methane yield. The results show that a two-step pretreatment, alkaline/enzymatic with a detoxification process, is necessary for the effective generation of high methane concentration biogas.
Ambrosia Beetles Prefer Closed Canopies: A Case Study in Oak Forests in Central Europe
Authors: Holuša Jaroslav , Fiala Tomáš , Foit Jiří
Published: September 2021

Research Highlights: The percentage of canopy closure was found to be the main factor associated with ambrosia beetle abundance and species richness. The latter two variables increased as canopy closure increased, probably because a high percentage of canopy closure provides a stable and humid environment suitable for the growth of ambrosia fungi. Objectives: Oak is a common host tree for ambrosia beetles (Coleoptera: Curculionidae: Scolytinae), which have independently evolved a nutritional mutualism with fungi. We suspected that ambrosia beetles might have specific habitat preferences that are different from those of other saproxylic beetles and that reflect the specific habitat preferences of their food, i.e., ambrosia fungi. Methods: We assessed ambrosia beetle abundance with ethanol-lured traps in five old-growth oak dominated forests and five managed oak dominated forests (one trap per forest) during the vegetation period in 2020. We determined whether ambrosia beetle abundance and species richness depend on forest type (managed vs. unmanaged), degree of canopy closure, abundance of oak trees, abundance of coarse deadwood, and abundance of dead oak branches. Results: In total, 4137 individuals of six species of ambrosia beetles associated with oaks were captured. The native ambrosia beetle Anisandrus dispar represented the majority of trapped ambrosia bark beetles. A. dispar along with another ambrosia beetle, Xyleborinus saxesenii, represented 99% of all captured beetles. Conclusions: In addition to canopy closure, the abundance of oak trees and the abundance of dead oak branches were significantly associated with ambrosia beetle abundance and species richness. The abundance of A. dispar was mainly correlated with dead oak branch abundance and the degree of canopy closure, whereas the abundances of X. saxesenii and of the invasive species Xyleborinus attenuatus and Cyclorhipidion bodoanum were mainly correlated with the net area occupied by oak trees.
Effects of Leaf Loss by Artificial Defoliation on the Growth of Different Poplar and Willow Varieties
Authors: Helbig Christiane E. , Müller Michael G. , Landgraf Dirk
Published: September 2021

The cultivation of fast-growing tree species in short rotation coppices has gained popularity in Germany in recent years. The resilience of these coppices to phyllophagous pest organisms is crucial for their profitable management, since the loss of a single annual increment can lead to uncompensable economic losses. To study the effects of leaf loss on the growth of poplar and willow varieties that are frequently cultivated under local conditions, three sample short rotation coppices including five poplar (Populus spp.) and three willow (Salix spp.) varieties were established in a randomized block design with four artificial defoliation variants and, on one site, with three different variants regarding the number of defoliation treatments. After up to three defoliation treatments within two growing seasons, the results show negative effects of leaf loss on the height growth and the fresh weight of the aboveground biomass of plants. Our data also suggests a lasting effect of defoliation on plant growth and re-growth after the end of the treatment. In general, defoliation had a greater impact on the growth of poplars than on willows. We conclude that even minor leaf loss can have an impact on plant growth but that the actual effects of defoliation clearly depend on the site, tree species, and variety as well as the extent and number of defoliations, which determine the ability of plants for compensatory growth.
Evaluation of Autochthonous Non-Saccharomyces Yeasts by Sequential Fermentation for Wine Differentiation in Galicia (NW Spain)
Authors: Blanco Pilar , Castrillo David , Graña María José , Lorenzo María José , Soto Elvira
Published: September 2021

Non-Saccharomyces yeasts constitute a useful tool in winemaking because they secrete hydrolytic enzymes and produce metabolites that enhance wine quality; in addition, their ability to reduce alcohol content and/or to increase acidity can help to mitigate the effects of climatic change on wines. The purpose of this study was to evaluate the oenological traits of non-Saccharomyces yeast strains autochthonous from Galicia (NW Spain). To do that, we carried out sequential fermentation using 13 different species from the yeast collection of Estación de Viticultura e Enoloxía de Galicia (Evega) and Saccharomyces cerevisiae EC1118. The fermentation kinetics and yeast implantation were monitored using conventional methods and genetic techniques, respectively. The basic chemical parameters of wine were determined using the OIV official methodology, and the fermentative aroma compounds were determined by GC–FID. The results evidenced the limited fermentative power of these yeasts and the differences in their survival after the addition of S. cerevisiae to complete fermentation. Some strains reduced the alcohol and/or increased the total acidity of the wine. The positive effect on sensory wine properties as well as the production of desirable volatile compounds were confirmed for Metschnikowia spp. (Mf278 and Mp176), Lachancea thermotolerans Lt93, and Pichia kluyveri Pkl88. These strains could be used for wine diversification in Galicia.
Bio-Mechanical and Bio-Rheological Aspects of Sickle Red Cells in Microcirculation: A Mathematical Modelling Approach
Authors: Chaturvedi Purnima , Kumar Rohit , Shah Sapna Ratan
Published: September 2021

Sickle cell disease (SCD) is an inherited monogenic disease characterized by distorted red blood cells that causes vaso-occlusion and vasculopathy. Presently, electrophoresis of haemoglobin and genotyping are used as routine tests for diagnosis of the SCD. These techniques require specialized laboratories and are expensive. The low-cost microfluidics-based diagnostic tool holds a great attention for screening of red blood cell (RBC) deformability. In the present study, lubrication theory has been applied in order to develop a biomechanical model of microcirculation with altered rheological properties of sickle blood in the capillary, which is smaller in size compared to the cell diameter, to explain the multifactorial nature and pathogenesis of vaso-occlusion in SCD. The governing equations have been solved analytically for realistic boundary conditions and simulated using MATLAB. We found that the axial velocity of the cell decreases with a decrease in deformability and compliance. The height of the lubricating film predicts deformation of the cell with respect to local pressure in the microcirculation. Leak back and drag force depend non-linearly on the deformed cell radius with varying viscosity of the plasma and Reynolds number. The modelling predictions of this study is in coherence with experimental results. The analyzed parameters provide unique insights with novel possibilities to design a microfluidics-based effective therapeutic intervention for SCD.
Development of Optimal Digesting Conditions for Microplastic Analysis in Dried Seaweed Gracilaria fisheri
Authors: Prihandari Rizky , Karnpanit Weeraya , Kittibunchakul Suwapat , Kemsawasd Varongsiri
Published: September 2021

Currently, research on the accumulation of microplastics (MPs) in the marine food web is being highlighted. An accurate and reliable digestion method to extract and isolate MPs from complex food matrices has seldom been validated. This study aimed to compare the efficacy of MP isolation among enzymatic-, oxidative-, and the combination of two digestion methods on red seaweed, Gracilaria fisheri. The dried seaweed sample was digested using three different methods under various conditions using enzymes (cellulase and protease), 30% H2O2, and a combination of enzymes and 30% H2O2. The method possessing the best digestion efficiency and polymer recovery rate of MPs was selected, and its effect on spiked plastic polymer integrity was analyzed by Raman spectroscopy. As a result, the enzymatic method rendered moderate digestion efficiency (59.3–63.7%) and high polymer recovery rate (94.7–98.9%). The oxidative method using 30% H2O2 showed high digestion efficiency (93.0–96.3%) and high polymer recovery rate (>98%). The combination method was the most effective method in terms of digestion efficiency, polymer recovery rate, and expenditure of digestion time. The method also showed no chemical changes in the spiked plastic polymers (PE, PP, PS, PVC, and PET) after the digestion process. All the spiked plastic polymers were identifiable using Raman spectroscopy.
The 10,000-Year Success Story of Wheat!
Authors: de Sousa Telma , Ribeiro Miguel , Sabença Carolina , Igrejas Gilberto
Published: September 2021

Wheat is one of the most important cereal crops in the world as it is used in the production of a diverse range of traditional and modern processed foods. The ancient varieties einkorn, emmer, and spelt not only played an important role as a source of food but became the ancestors of the modern varieties currently grown worldwide. Hexaploid wheat (Triticum aestivum L.) and tetraploid wheat (Triticum durum Desf.) now account for around 95% and 5% of the world production, respectively. The success of this cereal is inextricably associated with the capacity of its grain proteins, the gluten, to form a viscoelastic dough that allows the transformation of wheat flour into a wide variety of staple forms of food in the human diet. This review aims to give a holistic view of the temporal and proteogenomic evolution of wheat from its domestication to the massively produced high-yield crop of our day.
Effect of Biodegradable Hydrophilic and Hydrophobic Emulsifiers on the Oleogels Containing Sunflower Wax and Sunflower Oil
Authors: Bharti Deepti , Kim Doman , Cerqueira Miguel Angelo , Mohanty Biswaranjan , Habibullah SK , Banerjee Indranil , Pal Kunal
Published: September 2021

The use of an appropriate oleogelator in the structuring of vegetable oil is a crucial point of consideration. Sunflower wax (SFW) is used as an oleogelator and displays an excellent potential to bind vegetable oils. The current study aimed to look for the effects of hydrophobic (SPAN-80) and hydrophilic (TWEEN-80) emulsifiers on the oleogels prepared using SFW and sunflower oil (SO). The biodegradability and all formulations showed globular crystals on their surface that varied in size and number. Wax ester, being the most abundant component of SFW, was found to produce fibrous and needle-like entanglements capable of binding more than 99% of SO. The formulations containing 3 mg of liquid emulsifiers in 20 g of oleogels showed better mechanical properties such as spreadability and lower firmness than the other tested concentrations. Although the FTIR spectra of all the formulations were similar, which indicated not much variation in the molecular interactions, XRD diffractograms confirmed the presence of β′ form of fat crystals. Further, the mentioned formulations also showed larger average crystallite sizes, which was supported by slow gelation kinetics. A characteristic melting point (Tm~60 °C) of triglyceride was visualized through DSC thermograms. However, a higher melting point in the case of few formulations suggests the possibility of even a stable β polymorph. The formed oleogels indicated the significant contribution of diffusion for curcumin release. Altogether, the use of SFW and SO oleogels with modified properties using biodegradable emulsifiers can be beneficial in replacing saturated fats and fat-derived products.
Fine-Tuning of Molecular Structures to Generate Carbohydrate Based Super Gelators and Their Applications for Drug Delivery and Dye Absorption
Authors: Bietsch Jonathan , Olson Mary , Wang Guijun
Published: September 2021

Carbohydrate-based low molecular weight gelators (LMWGs) exhibit many desirable properties making them useful in various fields including applications as drug delivery carriers. In order to further understand the structural connection to gelation properties, especially the influence of halide substitutions, we have designed and synthesized a series of para-chlorobenzylidene acetal protected D-glucosamine amide derivatives. Fifteen different amides were synthesized, and their self-assembling properties were assessed in multiple organic solvents, as well as mixtures of organic solvents with water. All derivatives were found to be gelators for at least one solvent and majority formed gels in multiple solvents at concentrations lower than 2 wt%. A few derivatives rendered remarkably stable gels in aqueous solutions at concentrations below 0.1 wt%. The benzamide 13 formed gels in water and in EtOH/H2O (v/v 1:2) at 0.36 mg/mL. The gels were characterized using optical microscopy and atomic force microscopy, and the self-assembly mechanism was probed using variable temperature 1H-NMR spectroscopy. Gel extrusion studies using H2O/DMSO gels successfully printed lines of gels on glass slides, which retained viscoelasticity based on rheology. Gels formed by the benzamide 13 were used for encapsulation and the controlled release of chloramphenicol and naproxen, as well as for dye removal for toluidine blue aqueous solutions.
The Level of Self-Care among Patients with Chronic Heart Failure
Authors: Pobrotyn Piotr , Mazur Grzegorz , Kałużna-Oleksy Marta , Uchmanowicz Bartosz , Lomper Katarzyna
Published: September 2021

Introduction: In a long-term approach to the treatment of heart failure, importance is given to the process of self-care management and behaviors. The number of rehospitalizations and unscheduled medical visits can be reduced by actively engaging patients in the self-care process. Methods: The study included 403 patients with chronic heart failure (mean LVEF 40.53%), hospitalized in the Cardiology Department. Medical record analysis and a self-report questionnaire were used to obtain basic sociodemographic and clinical data. The European Heart Failure Self-care Behavior Scale, revised into a nine-item scale (EHFScBS-9), was used to evaluate self-care behavior. Results: Analysis of the EHFSc-9 self-care behavior scale showed that the mean score was 49.55 out of 100 possible points (SD = 22.07). Univariate analysis revealed that significant (p < 0.05) negative predictors of the EHFScB-9 self-care scale included: male sex (b = −5146), hospitalizations in the last year (b = −5488), NYHA class II (b = −11,797) and NYHA IV class (b = −15,196). The multivariate linear regression model showed that a significant (p ˂ 0.05) negative predictor of the EHFScB-9 self-care scale was male sex (b = −5.575). Conclusions: Patients with chronic HF achieve near optimal self-care behavior outcomes. A patient prepared to engage with self-care will have fewer rehospitalizations and a better quality of life.
The Impact of the Pandemic on Acute Ischaemic Stroke Endovascular Treatment from a Multidisciplinary Perspective: A Nonsystematic Review
Authors: Grigonyte Milda , Kraujelyte Agne , Januskeviciute Elija , Semys Giedrius , Kriukelyte Oresta , Kontrimaviciute Egle , Valeviciene Nomeda Rima , Jatuzis Dalius
Published: September 2021

Background: At the beginning of the coronavirus disease 2019 (COVID-19) pandemic, reduced admissions for cerebrovascular events were identified, but acute ischaemic stroke (AIS) has remained one of the leading causes of death and disability for many years. The aim of this article is to review current literature data for multidisciplinary team (MDT) coordination, rational management of resources and facilities, ensuring timely medical care for large vessel occlusion (LVO) AIS patients requiring endovascular treatment during the pandemic. Methods: A detailed literature search was performed in Google Scholar and PubMed databases using these keywords and their combinations: acute ischaemic stroke, emergency, anaesthesia, airway management, mechanical thrombectomy, endovascular treatment, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), COVID-19. Published studies and guidelines from inception to April 2021 were screened. The following nonsystematic review is based on a comprehensive literature search of available data, wherein 59 were chosen for detailed analysis. Results: The pandemic has an impact on every aspect of AIS care, including prethrombectomy, intraprocedural and post-thrombectomy issues. Main challenges include institutional preparedness, increased number of AIS patients with multiorgan involvement, different work coordination principles and considerations about preferred anaesthetic technique. Care of these patients is led by MDT and nonoperating room anaesthesia (NORA) principles are applied. Conclusions: Adequate management of AIS patients requiring mechanical thrombectomy during the pandemic is of paramount importance to maximise the benefit of the endovascular procedure. MDT work and familiarity with NORA principles decrease the negative impact of the disease on the clinical outcomes for AIS patients.