bluuebunny/crossref_metadata_2025 huggingface dataset:
Blood Tumor Mutational Burden as a Predictive Biomarker in Patients With Advanced Non-Small Cell Lung Cancer (NSCLC)
Authors: Ma Yuhui , Li Quan , Du Yaxi , Cai Jingjing , Chen Wanlin , Zhao Guangqiang , Liu Xing , Li Hongsheng , Ma Luyao , Huang Yunchao , Zhou Yongchun
Published: May 2021

This study was designed to investigate the impact of blood tumor mutational burden (bTMB) on advanced NSCLC in Southwest China. The relationship between the tTMB estimated by next-generation sequencing (NGS) and clinical outcome was retrospectively analyzed in tissue specimens from 21 patients with advanced NSCLC. Furthermore, the relationship between the bTMB estimated by NGS and clinical outcome was retrospectively assessed in blood specimens from 70 patients with advanced NSCLC. Finally, 13 advanced NSCLC patients were used to evaluate the utility of bTMB assessed by NGS in differentiating patients who would benefit from immunotherapy. In the tTMB group, tTMB ≥ 10 mutations/Mb was related to inferior progression-free survival (PFS) (hazard ratio [HR], 0.30; 95% CI, 0.08-1.17; log-rank P = 0.03) and overall survival (OS) (HR, 0.30; 95% CI, 0.08-1.16; log-rank P = 0.03). In the bTMB group, bTMB ≥ 6 mutations/Mb was associated with inferior PFS (HR, 0.32; 95% CI, 0.14-1.35; log-rank P < 0.01) and OS (HR, 0.31; 95% CI, 0.14-0.7; log-rank P < 0.01). In the immunotherapy section, bTMB ≥ 6 mutations/Mb was related to superior PFS (HR, 0.32; 95% CI, 0.14-1.35; log-rank P < 0.01) and objective response rates (ORRs) (bTMB < 6: 14.2%; 95% CI, 0.03-1.19; bTMB ≥ 6: 83.3%; 95% CI, 0.91-37.08; P = 0.02). These findings suggest that bTMB is a validated predictive biomarker for determining the clinical outcome of advanced NSCLC patients and may serve as a feasible predictor of the clinical benefit of immunotherapies (anti-PD-1 antibody) in the advanced NSCLC population in Yunnan Province.
Ultrasound-Guided Percutaneous Core Needle Biopsy of Peripheral Pulmonary Nodules ≤ 2 cm: Diagnostic Performance, Safety and Influence Factors
Authors: Huang Weijun , Ye Jieyi , Qiu Yide , Peng Weiwei , Lan Ninghui , Huang Ting , Ou Yinghui , Deng Xiaoyun , Li Yingjia
Published: May 2021

Purpose

To evaluate diagnostic performance and safety of ultrasound-guided needle biopsy in the diagnosis of peripheral pulmonary nodules (PPLs) ≤ 2 cm, and the influence factors of sample adequacy and safety.

Materials and Methods

194 patients (99 men, 95 women; mean age, 56.2 ± 13.7 years) who received biopsy for PPLs ≤ 2 cm between January 2014 to January 2019 were included. Variables including patient demographics, lesion location, lesion size, presence of lesion necrosis, presence of emphysema on CT, patient position, biopsy needle size and number of needle passes were recorded. Univariate analysis and multivariate logistic regression analysis were performed to explore the influence factor of sample adequacy and safety.

Results

Biopsy specimens were adequate for diagnosis in 161/194 (83%) cases; the diagnostic accuracy was 81.4% (158/194). The overall complication rate was 8.8% (17/194), including pneumothorax, hemoptysis and pleural effusion, which occurred in 2.1% (4/194), 5.2% (10/194), and 1.5% (3/194) of patients, respectively. The incidence of pneumothorax in the 16-gauge-needle group were significantly higher than that of the 18-gauge-needle group (5.6% vs 0%, P=0.018). Adequate sampling of 16-gauge and 18-gauge needles were achieved in 90.3%(65/72) and 78.7%(96/122) cases, respectively. Multivariate logistic regression analysis revealed needle size (16-gauge vs 18-gauge) was an independent influence factors of sample adequacy (P=0.015, odds ratio=3.419). A receiver operating characteristic curve was plotted and the area under the curve was 0.774.

Conclusion

US-guided percutaneous needle biopsy is a feasible and safe technique for small PPLs ≤ 2 cm. Needle size is an independent influence factor of sample adequacy and post-procedure pneumothorax. Sixteen-gauge needle has the advantage of achieving adequate sample for pathological analysis, though the risk of pneumothorax should be alerted.
Repurposing a PICU for Adult Care in a State Mandated COVID-19 Only Hospital: Outcome Comparison to the MICU Cohort to Determine Safety and Effectiveness
Authors: Gist Ramon E. , Pinto Rohit , Kissoon Niranjan , Ahmed Youssef E. , Daniel Pia , Hamele Mitchell
Published: May 2021

Objective: The ongoing coronavirus 2019 (COVID-19) pandemic is disproportionally impacting the adult population. This study describes the experiences after repurposing a PICU and its staff for adult critical care within a state mandated COVID-19 hospital and compares the outcomes to adult patients admitted to the institution's MICU during the same period.

Design: A retrospective chart review was performed to analyze outcomes for the adults admitted to the PICU and MICU during the 27-day period the PICU was incorporated into the institution's adult critical care surge plan.

Setting: Tertiary care state University hospital.

Patients: Critically ill adult patients with proven or suspected COVID-19.

Interventions: To select the most ideal adult patients for PICU admission a tiered approach that incorporated older patients with more comorbidities at each stage was implemented.

Measurements and Main Results: There were 140 patients admitted to the MICU and 9 patients admitted to the PICU during this period. The mean age of the adult patients admitted to the PICU was lower (49.1 vs. 63.2 p = 0.017). There was no statistically significant difference in the number of comorbidities, intubation rates, days of ventilation, dialysis or LOS. Patients selected for PICU care did not have coronary artery disease, CHF, cerebrovascular disease or COPD. Mean admission Sequential Organ Failure Assessment (SOFA) score was lower in patients admitted to the PICU (4 vs. 6.4, p = 0.017) with similar rates of survival to discharge (66.7 vs. 44.4%, p = 0.64).

Conclusion: Outcomes for the adult patients who received care in the PICU did not appear to be worse than those who were admitted to the MICU during this time. While limited by a small sample size, this single center cohort study revealed that careful assessment of critical illness considering age and type of co-morbidities may be a safe and effective approach in determining which critically ill adult patients with known or suspected COVID-19 are the most appropriate for PICU admission in general hospitals with primary management by its physicians and nurses.
Influence of NaCl on the Structure and Dynamics of Phospholipid Layers
Authors: Jaksch Sebastian , Holderer Olaf , Frielinghaus Henrich , Koutsioubas Alexandros , Zolnierczuk Piotr , Hayward Dominic William , Förster Stephan , Müller-Buschbaum Peter
Published: May 2021

We present a structural and dynamical analysis of the influence of NaCl on multilayer stacks of phospholipids on a solid surface. To this end, multilayer stacks of phospholipids (L-α-phosphatidylcholine, abbreviated as SoyPC) are investigated with neutron reflectometry, grazing-incidence small-angle neutron scattering (GISANS) and grazing-incidence neutron-spin echo spectroscopy (GINSES). We show both that the NaCl influence on the structure is predominantly on water-head group interface and also, that the change in dynamics is restricted to an associated change in the inter-plane viscosity. Using this knowledge, it is possible to model the dynamical behavior of a phospholipid membrane in response to a salt concentration of the solvent using only a single parameter, namely the in-plane viscosity. The excellent agreement with our previously published model also strongly supports the existence of a thermally excited surface mode in phospholipid membranes for close-to-physiological conditions.
The Angiotensin II Type 1 Receptor-Associated Protein Attenuates Angiotensin II-Mediated Inhibition of the Renal Outer Medullary Potassium Channel in Collecting Duct Cells
Authors: Polidoro Juliano Zequini , Rebouças Nancy Amaral , Girardi Adriana Castello Costa
Published: May 2021

Adjustments in renal K+ excretion constitute a central mechanism for K+ homeostasis. The renal outer medullary potassium (ROMK) channel accounts for the major K+ secretory route in collecting ducts during basal conditions. Activation of the angiotensin II (Ang II) type 1 receptor (AT1R) by Ang II is known to inhibit ROMK activity under the setting of K+ dietary restriction, underscoring the role of the AT1R in K+ conservation. The present study aimed to investigate whether an AT1R binding partner, the AT1R-associated protein (ATRAP), impacts Ang II-mediated ROMK regulation in collecting duct cells and, if so, to gain insight into the potential underlying mechanisms. To this end, we overexpressed either ATRAP or β-galactosidase (LacZ; used as a control), in M-1 cells, a model line of cortical collecting duct cells. We then assessed ROMK channel activity by employing a novel fluorescence-based microplate assay. Experiments were performed in the presence of 10−10 M Ang II or vehicle for 40 min. We observed that Ang II-induced a significant inhibition of ROMK in LacZ, but not in ATRAP-overexpressed M-1 cells. Inhibition of ROMK-mediated K+ secretion by Ang II was accompanied by lower ROMK cell surface expression. Conversely, Ang II did not affect the ROMK-cell surface abundance in M-1 cells transfected with ATRAP. Additionally, diminished response to Ang II in M-1 cells overexpressing ATRAP was accompanied by decreased c-Src phosphorylation at the tyrosine 416. Unexpectedly, reduced phospho-c-Src levels were also found in M-1 cells, overexpressing ATRAP treated with vehicle, suggesting that ATRAP can also downregulate this kinase independently of Ang II-AT1R activation. Collectively, our data support that ATRAP attenuates inhibition of ROMK by Ang II in collecting duct cells, presumably by reducing c-Src activation and blocking ROMK internalization. The potential role of ATRAP in K+ homeostasis and/or disorders awaits further investigation.
Effect of Nitrogen Addition on Selection of Germination Trait in an Alpine Meadow on the Tibet Plateau
Authors: Liu Kun , Liu Yang , Zhang Zhilong , Zhang Shiting , Baskin Carol C. , Baskin Jerry M. , Liang Ting , Bu Haiyan , Li Shuxia , Zhang Tingting , Cui Xianliang , Xiao Sa
Published: May 2021

Seed germination requirements may determine the kinds of habitat in which plants can survive. We tested the hypothesis that nitrogen (N) addition can change seed germination trait-environmental filter interactions and ultimately redistribute seed germination traits in alpine meadows. We determined the role of N addition on germination trait selection in an alpine meadow after N addition by combining a 3-year N addition experiment in an alpine meadow and laboratory germination experiments. At the species level, germination percentage, germination rate (speed) and breadth of temperature niche for germination (BTN) were positively related to survival of a species in the fertilized community. In addition, community-weighted means of germination percentage, germination rate, germination response to alternating temperature and BTN increased. However, germination response to wet-cold storage (cold stratification) and functional richness of germination traits was lower in alpine meadows with high-nitrogen addition than in those with no, low and medium N addition. Thus, N addition had a significant influence on environmental filter-germination trait interactions and generated a different set of germination traits in the alpine meadow. Further, the effect of N addition on germination trait selection by environmental filters was amount-dependent. Low and medium levels of N addition had less effect on redistribution of germination traits than the high level.
Extensins: Self-Assembly, Crosslinking, and the Role of Peroxidases
Authors: Mishler-Elmore John W. , Zhou Yadi , Sukul Abhijit , Oblak Mercedes , Tan Li , Faik Ahmed , Held Michael A.
Published: May 2021

The extensin (EXT) network is elaborated by the covalent intermolecular crosslinking of EXT glycoprotein monomers, and its proper assembly is important for numerous aspects of basic wall architecture and cellular defense. In this review, we discuss new advances in the secretion of EXT monomers and the molecular drivers of EXT network self-assembly. Many of the functions of EXTs are conferred through covalent crosslinking into the wall, so we also discuss the different types of known intermolecular crosslinks, the enzymes that are involved, as well as the potential for additional crosslinks that are yet to be identified. EXTs also function in wall architecture independent of crosslinking status, and therefore, we explore the role of non-crosslinking EXTs. As EXT crosslinking is upregulated in response to wounding and pathogen infection, we discuss a potential regulatory mechanism to control covalent crosslinking and its relationship to the subcellular localization of the crosslinking enzymes.
Delineating the Benefits of Arts Education for Children’s Socioemotional Development
Authors: Holochwost Steven J. , Goldstein Thalia R. , Wolf Dennie Palmer
Published: May 2021

In this paper, we argue that in order for the study of arts education to continue to advance, we must delineate the effects of particular forms of arts education, offered in certain contexts, on specific domains of children’s socioemotional development. We explain why formulating precise hypotheses about the effects of arts education on children’s socioemotional development requires a differentiated definition of each arts education program or activity in question, as well as a consideration of both the immediate and broader contexts in which that program or activity occurs. We then offer the New Victory Theater’s Schools with Performing Arts Reach Kids (SPARK) program as an illustrative example of how these considerations allow for the refinement of hypotheses about the impact of arts education on children’s socioemotional development.
The Polish Version of the Resilience Scale 25: Adaptation and Preliminary Psychometric Evaluation
Authors: Konaszewski Karol , Skalski Sebastian , Surzykiewicz Janusz
Published: May 2021

Background: The aim of the presented series of studies was to test the factor structure and assess the psychometric properties of the Resilience Scale 25 in the Polish population. It was developed and tested during the course of four independent studies analysing various aspects of the validation of the RS 25 questionnaire’s Polish version.

Method: Study 1 concerned the procedure for developing the Polish language version. Study 2 (N = 2716) consisted of reliability tests and a confirmatory factor analysis. In Studies 3 (N = 733) and 4 (N = 431), the validity was assessed by examining the relationship between resilience and the assessment of ego-resiliency, the risk of depression, styles of coping with stress, perceived stress, and satisfaction with life.

Results: The presented research results obtained using the measure indicate that it can be considered to be a reliable and valid research tool. A five-factor solution showed a good fit to the data: χ2/df = 12.85; RMSEA = 0.066 (low = 0.064; high = 0.068; 90% CI); GFI = 0.90; AGFI = 0.90. An assessment of the internal consistency was carried out on the basis of Cronbach’s alpha. The values achieved were satisfactory and indicate acceptable internal reliability of the questionnaire (0.89) and of the five dimensions: (1) purpose (0.65); (2) equanimity (0.65); (3) self-reliance (0.75); (4) perseverance (0.72); and (5) existential aloneness (0.66). In accordance with the predictions and earlier studies, resilience was correlated positively with ego-resiliency, a task-oriented style of coping with stress, and life satisfaction and negatively with perceived stress, the risk of depression, and an emotion-oriented coping style.

Conclusion: The Polish version of the RS 25 allows the assessment of the intensity of resilience as a general indicator and its five constituting dimensions. Such a measurement seems to be important from the perspective of assessing the role of an individual’s resources in clinical psychology, health psychology, and psychotherapy.
Patients Characteristics and Psychosocial Treatment in Psychodynamic and Cognitive Behavior Therapy
Authors: Muschalla Beate , Linden Michael , Rose Matthias
Published: May 2021

Introduction: The most prevalent psychotherapy schools are psychodynamic (PDT) and cognitive behavior therapy (CBT). There are no scientific guidelines on which type of patient should be treated by which therapist, and how they can find the best one. Part of the answer can be derived from data on who is treated in which way.

Objective: Objective of this study was to compare patients in routine PDT and CBT to describe similarities and differences in regard to patient status and treatment.

Materials and Methods: A research psychotherapist visited 73 cognitive behavior therapists and 58 psychodynamic psychotherapists in their office and asked them to report about the last cases they had seen. There were 188 CBT and 134 PD case reports.

Results: There were no significant differences in socio-demographics between PDT and CBT patients. The average number of treatment session so far was significantly higher in PDT than CBT. There were longer duration of illness, more parallel medical treatments and higher rates of sick leave in CBT patients. While assessment of capacities with the Mini-ICF-APP showed no differences, more participation restrictions were found in CBT patients. Correspondingly there were more sociomedical interventions, especially in regard to work.

Conclusions: The differences between PDT and CBT may be explained by the fact that PDT requires analytical capabilities on the side of the patient, which may exclude patients with social problems, while CBT is coping oriented which allows the inclusion of all kinds of patients. Still, in both treatment modes, complex patients are treated with multidimensional interventions.
Price Variance in Hybrid-LCA Leads to Significant Uncertainty in Carbon Footprints
Authors: Jakobs Arthur , Schulte Simon , Pauliuk Stefan
Published: May 2021

Hybrid Life Cycle Assessment (HLCA) methods attempt to address the limitations regarding process coverage and resolution of the more traditional Process- and Input-Output Life Cycle Assessments (PLCA, IOLCA). Due to the use of different units, HLCA methods rely on commodity price information to convert the physical units used in process inventories to the monetary units commonly used in Input-Output models. However, prices for the same commodity can vary significantly between different supply chains, or even between various levels in the same supply chain. The resulting commodity price variance in turn leads to added uncertainty in the hybrid environmental footprint. In this paper we take international trading statistics from BACI/UN-COMTRADE to estimate the variance of commodity prices, and use these in an integrated HLCA model of the process database ecoinvent with the EE-MRIO database EXIOBASE. We show that geographical aggregation of PLCA processes is a significant driver in the price variance of their reference products. We analyse the effect of price variance on process carbon footprint intensities (CFIs) and find that the CFIs of hybridised processes show a median increase of 6–17% due to hybridisation, for two different double counting scenarios, and a median uncertainty of −2 to +4% due to price variance. Furthermore, we illustrate the effect of price variance on the carbon footprint uncertainty in a HLCA study of Swiss household consumption. Although the relative footprint increase due to hybridisation is small to moderate with 8–14% for two different double counting correction strategies, the uncertainty due to price variability of this contribution to the footprint is very high, with 95% confidence intervals of (−28, +90%) and (−23, +68%) relative to the median. The magnitude and high positive skewness of the uncertainty highlights the importance of taking price variance into account when performing hybrid LCA.
Clostridium difficile: Bacteria That Can Infect People Taking Antibiotics
Authors: Delmée Michel
Published: May 2021

If I ask you what antibiotics are for, you will probably say that they are used to treat infections, by killing the bacteria responsible for those infections. Well, in this article I will explain to you that there are some circumstances in which antibiotic treatment may instead cause an infection. The microbe responsible for this infection is called Clostridium difficile. I will explain which is this curious microbe, how and when it causes an infection, why antibiotics are implicated and how we can treat it …
An Analysis of Broadcasting Media Using Social Media Engagement in the WNBA
Authors: Pegoraro Ann , Kennedy Heather , Agha Nola , Brown Nicholas , Berri David
Published: May 2021

While there has been research into what teams, leagues, and athletes post on social media and the impact of post content on social media engagement, there is limited understanding and empirical research on the impact of broadcasting media on social sport consumption. There are an increasing number of new media through which sport leagues can distribute their content to fans. This research examines the impact of different broadcast platforms on game day engagement with WNBA team Twitter accounts. Using data for the 2016–2018 seasons, results indicate athlete/team quality and performance were positively associated with post engagement, underscoring the importance of the core sport product and potentially indicating that the WNBA is developing a star-driven culture similar to the NBA. In addition, broadcasting on League Pass or local TV (for home teams) and Twitter were associated with lower post engagement suggesting we have more to learn about maximizing online engagement.
Perspective on Clinically-Relevant Antimicrobial Resistant Enterobacterales in Food: Closing the Gaps Using Genomics
Authors: Díaz-Gavidia Constanza , Álvarez Francisca P. , Munita Jose M. , Cortés Sandra , Moreno-Switt Andrea I.
Published: May 2021

Antimicrobial resistance is one of the most important public health concerns—it causes 700,000 deaths annually according to the World Health Organization (WHO). Enterobacterales such as E. coli and Klebsiella pneumoniae, have become resistant to many relevant antimicrobials including carbapenems and extended spectrum cephalosporins. These clinically relevant resistant Enterobacterales (CRRE) members are now globally distributed in the environment including different food types (meats, produce, dairy). Unlike known foodborne pathogens, CRRE are not usually part of most food surveillance systems. However, numerous reports of CRRE highlight the importance of these bacteria in food and have been shown to contribute to the overall crisis of antimicrobial resistance. This is especially important in the context of carriage of these pathogens by immuno-compromised individuals. CRRE infections upon consumption of contaminated food could colonize the human gastrointestinal tract and eventually be a source of systemic infections such as urinary tract infections or septicemia. While different aspects need to be considered to elucidate this, whole genome sequencing along with metadata could be used to understand genomic relationships of CRRE obtained from foods and humans, including isolates from clinical infections. Once robust scientific data is available on the role of CRRE in food, countries could move forward to better survey and control CRRE in food.
El análisis transaccional en la era de la información.
Authors: Steiner Claude M.
Published: May 2021

El Análisis Transaccional tal como fue desarrollado por Eric Berne constituyó una teoría avanzada a su tiempo: además de ofrecer un enfoque altamente eficaz en psicoterapia anticipó cuestiones teóricas desde el punto de vista psicológico y psicoterapéutico que llegarían a ser importantes en la Era de la Información.
One Health Approach: An Overview of Q Fever in Livestock, Wildlife and Humans in Asturias (Northwestern Spain)
Authors: Espí Alberto , del Cerro Ana , Oleaga Álvaro , Rodríguez-Pérez Mercedes , López Ceferino M. , Hurtado Ana , Rodríguez-Martínez Luís D. , Barandika Jesús F. , García-Pérez Ana L.
Published: May 2021

This study aimed to investigate the seroprevalence of C. burnetii in domestic ruminants, wild ungulates, as well as the current situation of Q fever in humans in a small region in northwestern Spain where a close contact at the wildlife–livestock–human interface exists, and information on C. burnetii infection is scarce. Seroprevalence of C. burnetii was 8.4% in sheep, 18.4% in cattle, and 24.4% in goats. Real-time PCR analysis of environmental samples collected in 25 livestock farms detected Coxiella DNA in dust and/or aerosols collected in 20 of them. Analysis of sera from 327 wild ungulates revealed lower seroprevalence than that found in domestic ruminants, with 8.4% of Iberian red deer, 7.3% chamois, 6.9% fallow deer, 5.5% European wild boar and 3.5% of roe deer harboring antibodies to C. burnetii. Exposure to the pathogen in humans was determined by IFAT analysis of 1312 blood samples collected from patients admitted at healthcare centers with Q fever compatible symptoms, such as fever and/or pneumonia. Results showed that 15.9% of the patients had IFAT titers ≥ 1/128 suggestive of probable acute infection. This study is an example of a One Health approach with medical and veterinary institutions involved in investigating zoonotic diseases.
Bayesian Estimation of the True Seroprevalence and Risk Factor Analysis of Bovine Leukemia Virus Infection in Pakistan
Authors: Sakhawat Ali , Rola-Łuszczak Marzena , Osiński Zbigniew , Bibi Nazia , Kuźmak Jacek
Published: May 2021

The objective of this study was to determine the true seroprevalence of bovine leukemia virus (BLV) infection in dairy cattle from Pakistan at the animal and herd-level. We tested 1380 dairy cattle from 451 herds and 92 water buffalo. The sera were tested by ELISA and the results were analyzed using Bayesian inference. The median posterior estimate of the herd level true BLV prevalence was 1.4%, with a 95% credible interval (CI) 0.7–3.1, whereas the median posterior estimate of the within-farm true seroprevalence was 3.8% with a 95% CI 2.8–4.8. All 92 sera collected from water buffalo were negative. Several risk factors potentially associated with seropositivity to BLV infections in Pakistan were analyzed using logistic regression model based on calculation of an odds ratio (OR). The study showed an association between seropositivity and medium herd (≥50) size (OR = 23.57, 95% CI: 3.01–103.48). Common housing of indigenous cattle with exotic-breed cattle (OR = 0.67, 95% CI: 06–2.35) or housing indigenous or their crossbred cattle with exotic-breed cattle (OR = 0.95, 95% CI: 0.14–3.01) had no effect on the BLV seroprevalence. Similarly, common housing of cattle and water buffalo was not risk factor for increased BLV seropositivity (OR = 27.10, 95% CI: 0.63–119.34).
Heterologous Expression and Characterization of a Ferulic Acid Esterase from Aspergillus aculeatus with Potential Use in Sunflower Seed Processing
Authors: Zhang Wenbin , Wang Jieyu , Liu Yuechen , Leng Juncai
Published: May 2021

Hydrolytic activity for chlorogenic acid (CGA) has been recognized as an important side activity of some types of ferulic acid esterases. The purpose of this work was to enhance the efficient expression of ferulic acid esterase (FAE) and to explore its application in the processing of sunflower seed. Two novel FAEs from Aspergillus aculeatus (AaSD14) were expressed in genetically engineered E. coli BL21 (DE3), and their properties, including temperature, pH, metal ions and substrate specificity, were characterized after purification. Competitive CGA hydrolysis activity was observed in these recombined ferulic acid esterases (reFAEs) with reFAE1 of 246.37 U/g and reFAE2 of 340.95 U/g, which were 56.6 and 78.4 times higher than that of the wild strain (4.35 U/g), respectively. Meanwhile, the fermentation cycle was greatly shortened to 2.0 d. These reFAEs were recognized as type C FAE through substrate specificity assays. Treatment of sunflower seed protein (SSP) using reFAE2 resulted in a remarkable color change, from green to milk-white, confirming the activity of CGA biodegradation. Therefore, it shows certain potential in the processing of sunflower seed and other related foodstuffs.
In-Vitro Comparative Adhesion Evaluation of Bioceramic and Dual-Cure Resin Endodontic Sealers Using SEM, AFM, Push-Out and FTIR
Authors: Chisnoiu Radu Marcel , Moldovan Marioara , Prodan Doina , Chisnoiu Andrea Maria , Hrab Dana , Delean Ada Gabriela , Muntean Alexandrina , Rotaru Doina Iulia , Pastrav Ovidiu , Pastrav Mihaela
Published: May 2021

(1) Background: Which are the adhesion characteristics of four root canal sealers: two experimental (dual cure resin based endodontic sealer (E1-DCR) and bioceramic based sealer (E2-BC)), and two commercial (RealSeal and TotallFill BC (FKG Germany)). (2) Methods. One-hundred-twenty extracted monoradicular teeth received mechanic-antiseptic preparation and then endodontic filling using gutta-percha in combination with one of the four sealers. Samples from the apical third were selected from each tooth and were randomly distributed for Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM) and push-out bond strength. Fourier Transform Infrared (FTIR) Spectroscopy was made exclusively for experimental sealers. (3) Results. The two experimental materials did not present a significant difference in apical sealing ability based on SEM evaluation. Gaps were identified in case of RealSeal samples and also lower penetration into the dentinal tubules compared to TotalFill BC. On AFM analysis a more pronounced penetration into the tubules was observed in case of E2-BC sealer was observed. E1-DCR registered a lower bond strength (0.7177 ± 0.55) in the apical third compared to Total Fill BC (p = 0.01). (4) Conclusions. Within the limitations of the current study, it can be stated that the two experimental sealers’ characteristics are comparable to that of the two consecrated materials.
Privacy and Security in Cognitive Cities: A Systematic Review
Authors: Machin Juvenal , Batista Edgar , Martínez-Ballesté Antoni , Solanas Agusti
Published: May 2021

The emerging paradigm of the cognitive city, which augments smart cities with learning and behavioral change capabilities, is gaining increasing attention as a promising solution to the challenges of future mega-cities. Cognitive cities are built upon artificial learning and behavioral analysis techniques founded on the exploitation of human-machine collective intelligence. Hence, cognitive cities rely on the sharing of citizens’ daily-life data, which might be considered sensitive personal data. In this context, privacy and security of the shared information become critical issues that have to be addressed to guarantee the proper deployment of cognitive cities and the fundamental rights of people. This article provides a thorough literature review using the recommendations for systematic reviews proposed by Vom Brocke et al. and the PRISMA statement. We analyze peer-reviewed publications indexed in ACM Digital Library, IEEE Xplore, Scopus, and Web of Science until July 2020. We identify the main challenges on privacy and information security within cognitive cities, and the proposals described in the literature to address them. We conclude that many challenges remain open and we suggest several research lines that will require further examination in the years to come.
Application of Machine Learning Algorithms to Predict the Effectiveness of Radial Jet Drilling Technology in Various Geological Conditions
Authors: Kochnev Aleksandr , Galkin Sergey , Krivoshchekov Sergey , Kozyrev Nikita , Chalova Polina
Published: May 2021

This study presents a methodological approach to forecasting the efficiency of radial drilling technology under various geological and physical conditions. The approach is based upon the integration of mathematical statistical methods and building machine learning models to forecast the liquid production rate increment, as well as to forecast technological indexes using a hydrodynamic model. This paper reviewed the global practice of radial drilling and well intervention efficiency modeling. The efficiency of the technology in question was analyzed on the oil deposits of the Perm Territory. Mathematical statistical methods were used to determine the geological and technological parameters of the efficient technology use. Based on the determined parameters, machine learning models were built, allowing us to forecast the oil and liquid production rate. A script was developed to integrate machine learning methods into a hydrodynamic simulator. When the method was tested, the deviations in the difference between the actual and the forecast cumulative oil production did not exceed 10%, which proves the reliability of the method. At the same time, the hydrodynamic model allows for taking into account the mutual influence of oil wells, the dynamics of water cut, and reservoir pressure.
Development of Ground Special Vehicle PHM with Case-Based Reason Model
Authors: Wu Qicai , Yuan Haiwen , Yuan Haibin
Published: May 2021

The case-based reasoning (CBR) method can effectively predict the future health condition of the system based on past and present operating data records, so it can be applied to the prognostic and health management (PHM) framework, which is a type of data-driven problem-solving. The establishment of a CBR model for practical application of the Ground Special Vehicle (GSV) PHM framework is in great demand. Since many CBR algorithms are too complicated in weight optimization methods, and are difficult to establish effective knowledge and reasoning models for engineering practice, an application development using a CBR model that includes case representation, case retrieval, case reuse, and simulated annealing algorithm is introduced in this paper. The purpose is to solve the problem of normal/abnormal determination and the degree of health performance prediction. Based on the proposed CBR model, optimization methods for attribute weights are described. State classification accuracy rate and root mean square error are adopted to setup objective functions. According to the reasoning steps, attribute weights are trained and put into case retrieval; after that, different rules of case reuse are established for these two kinds of problems. To validate the model performance of the application, a cross-validation test is carried on a historical data set. Comparative analysis of even weight allocation CBR (EW-CBR) method, correlation coefficient weight allocation CBR (CW-CBR) method, and SA weight allocation CBR (SA-CBR) method is carried out. Cross-validation results show that the proposed method can reach better results compared with the EW-CBR model and CW-CBR model. The developed PHM framework is applied to practical usage for over three years, and the proposed CBR model is an effective approach toward the best PHM framework solutions in practical applications.
Characterization of PM10-Bound Polycyclic Aromatic Hydrocarbons and Associated Carcinogenic Risk in Bangkok, Thailand
Authors: Tadsanaprasittipol Amornphat , Tonrub Pinthong , Beverland Iain J. , Kalin Robert M.
Published: May 2021

Concentrations of ambient particulate-bound polycyclic aromatic hydrocarbons (pPAHs) were measured in PM10 samples collected at roadside, industrial and urban background sites in Bangkok between May 2013 and May 2014. The annual average PM10 concentrations were not significantly different between the roadside (56.4 ± 27.3 µg m−3) and industrial (51.0 ± 31.1 µg m−3) sites. The lowest annual mean PM10 was observed at the urban background site (39.8 ± 22.2 µg m−3). Seasonal variations of pPAHs were observed at the three sampling sites. The total pPAHs ranged between 1.09 and 13.10 ng m−3 (mean 4.85 ± 2.51 ng m−3), 1.49 and 9.39 ng m−3 (mean 3.84 ± 2.01 ng m−3) and 0.77 and 5.20 ng m−3 (mean 2.28 ± 1.16 ng m−3) at the roadside, industrial and urban background sites, respectively. The observed annual average benzo[a]pyrene concentrations were 0.47 ± 0.39 ng m−3, 0.35 ± 0.27 ng m−3 and 0.24 ± 0.19 ng m−3 at the roadside, industrial and urban background sites. Long-term carcinogenic health risk of inhalation exposure expressed as the toxicity equivalent to benzo[a]pyrene concentrations were calculated as 0.83, 0.72 and 0.39 ng m−3 at the industrial, roadside and urban background sites, respectively. The composition of pPAHs plays an important role in the carcinogenicity of a PAHs mixture.
Fuzzy Graph Learning Regularized Sparse Filtering for Visual Domain Adaptation
Authors: Min Lingtong , Zhou Deyun , Li Xiaoyang , Lv Qinyi , Zhi Yuanjie
Published: May 2021

Distribution mismatch can be easily found in multi-sensor systems, which may be caused by different shoot angles, weather conditions and so on. Domain adaptation aims to build robust classifiers using the knowledge from a well-labeled source domain, while applied on a related but different target domain. Pseudo labeling is a prevalent technique for class-wise distribution alignment. Therefore, numerous efforts have been spent on alleviating the issue of mislabeling. In this paper, unlike existing selective hard labeling works, we propose a fuzzy labeling based graph learning framework for matching conditional distribution. Specifically, we construct the cross-domain affinity graph by considering the fuzzy label matrix of target samples. In order to solve the problem of representation shrinkage, the paradigm of sparse filtering is introduced. Finally, a unified optimization method based on gradient descent is proposed. Extensive experiments show that our method achieves comparable or superior performance when compared to state-of-the-art works.
Overview of Low-Level Wind Shear Characteristics over Chinese Mainland
Authors: Lin Caiyan , Zhang Kaijun , Chen Xintao , Liang Sheng , Wu Junjie , Zhang Wei
Published: May 2021

The characteristics of low-level wind shear (LLWS) over the Chinese mainland were investigated using reports from pilots, air traffic controllers and the number of arriving/departing flights from 2016 to 2020. A preliminary analysis of the impact of the flights on the LLWS reports was carried out, and the cause of LLWS was also investigated. LLWS reports from most airports indicate that LLWS is most likely to occur within 600 m AGL with a higher density below 300 m, but with some exceptions, as wind shear is reported at higher altitudes at some airports. Airports with a high frequency of LLWS reports are almost all located in or around regions with complex topography and in regions with prevailing weather conditions favorable to LLWS. The variation in overall LLWS reports displays a steady increase from 2016 to 2019 and a decrease from 2019 to 2020, consistent with the trend in the number of flights, but with no evidently similar trends for individual airports. Seasonal variations in LLWS reports are observed and demonstrate no notable impact caused by the number of flights, implying that the main cause is the monthly variation of weather conditions. Diurnal variation is also evident and largely associated with the variation in number of flights during the busy period in addition to weather conditions, such as common strong winds, in the afternoon.
An fNIRS Investigation of Masculinity, Femininity, and Sex on Nonparents’ Empathic Response to Infant Cries
Authors: Ng Xinyao , Ng Li Ying , Gabrieli Giulio , Azhari Atiqah , Neoh Michelle Jin Yee , Esposito Gianluca
Published: May 2021

According to societal stereotypes, the female sex and people who are more feminine have been considered to be more empathic than males and people who are more masculine. Therefore, females and feminine individuals are expected to respond more empathically to an infant’s cries. While this hypothesis was tested using self-report scales, it has not been explored thoroughly in terms of prefrontal cortex (PFC) activity, which may be a more objective means of measuring empathy. Specifically, the medial PFC (mPFC) is involved in social cognitive processing and thus a good proxy to measure the level of empathy. This study aims to (1) assess if the empathic response, in terms of medial PFC (mPFC) activity, to infant cries differ between sexes; (2) investigate if the empathic response is moderated by levels of masculinity and femininity. Functional near-infrared spectroscopy (fNIRS) was used to measure nonparent participants’ (18 males, 20 females) mPFC response to infant cries of different pitches (high and low). The Toronto Empathy Questionnaire was used to measure trait empathy and Bem’s Sex Role Inventory was used to measure the level of masculinity and femininity. Results revealed that biological sex had no significant effect on the empathic response towards infant cries of varying pitch. Furthermore, masculinity, not femininity, was correlated with an increase in empathic response in the mPFC to high but not low-pitch infant cries. We reason that this is because of the higher aversiveness and inflicted pain associated with higher-pitched cries, which induces more emotional and physical pain that masculine individuals seek to avoid. Overall, the results suggest that greater masculinity would imply greater mentalizing and processing of empathy-related information.
The Performance of Fibrous CDC Electrodes in Aqueous and Non-Aqueous Electrolytes
Authors: Malmberg Siret , Arulepp Mati , Laanemets Krista , Käärik Maike , Laheäär Ann , Tarasova Elvira , Vassiljeva Viktoria , Krasnou Illia , Krumme Andres
Published: May 2021

The aim of this study was to investigate the electrochemical behaviour of aqueous electrolytes on thin-layer (20 µm) nanoporous carbide-derived carbon (CDC) composite fibrous directly electrospun electrodes without further carbonisation. There have been previously investigated fibrous electrodes, which are produced by applying different post-treatment processes, however this makes the production of fibrous electrodes more expensive, complex and time consuming. Furthermore, in the present study high specific capacitance was achieved with directly electrospun nanoporous CDC-based fibrous electrodes in different neutral aqueous electrolytes. The benefit of fibrous electrodes is the advanced mechanical properties compared to the existing commercial electrode technologies based on pressure-rolled or slurry-cast powder mix electrodes. Such improved mechanical properties are preferred in more demanding applications, such as in the space industry. Electrospinning technology also allows for larger electrode production capacities without increased production costs. In addition to the influence of aqueous electrolyte chemical composition, the salt concentration effects and cycle stability with respect to organic electrolytes are investigated. Cyclic voltammetry (CV) measurements on electrospun electrodes showed the highest capacitance for asymmetrical cells with an aqueous 1 M NaNO3-H2O electrolyte. High CV capacitance was correlated with constant current charge–discharge (CC) data, for which a specific capacitance of 191 F g−1 for the positively charged electrode and 311 F g−1 for the negatively charged electrode was achieved. The investigation of electrolyte salt concentration on fibrous electrodes revealed the typical capacitance dependence on ionic conductivity with a peak capacitance at medium concentration levels. The cycle-life measurements of selected two-electrode test cells with aqueous and non-aqueous electrolytes revealed good stability of the electrospun electrodes.
Novel Pharmacological Options in the Treatment of Cholangiocarcinoma: Mechanisms of Resistance
Authors: Marin Jose J. G. , Sanchon-Sanchez Paula , Cives-Losada Candela , del Carmen Sofía , González-Santiago Jesús M. , Monte Maria J. , Macias Rocio I. R.
Published: May 2021

Despite the crucial advances in understanding the biology of cholangiocarcinoma (CCA) achieved during the last decade, very little of this knowledge has been translated into clinical practice. Thus, CCA prognosis is among the most dismal of solid tumors. The reason is the frequent late diagnosis of this form of cancer, which makes surgical removal of the tumor impossible, together with the poor response to standard chemotherapy and targeted therapy with inhibitors of tyrosine kinase receptors. The discovery of genetic alterations with an impact on the malignant characteristics of CCA, such as proliferation, invasiveness, and the ability to generate metastases, has led to envisage to treat these patients with selective inhibitors of mutated proteins. Moreover, the hope of developing new tools to improve the dismal outcome of patients with advanced CCA also includes the use of small molecules and antibodies able to interact with proteins involved in the crosstalk between cancer and immune cells with the aim of enhancing the immune system’s attack against the tumor. The lack of effect of these new therapies in some patients with CCA is associated with the ability of tumor cells to continuously adapt to the pharmacological pressure by developing different mechanisms of resistance. However, the available information about these mechanisms for the new drugs and how they evolve is still limited.
Investigating the Impact of Immune-Related Adverse Events, Glucocorticoid Use and Immunotherapy Interruption on Long-Term Survival Outcomes
Authors: Bruyère Charline Lafayolle de la , Souquet Pierre-Jean , Dalle Stéphane , Corbaux Pauline , Boespflug Amélie , Duruisseaux Michaël , Kiakouama-Maleka Lize , Reverdy Thibaut , Maugeais Madeleine , Sahin Gulsum , Maillet Denis , Péron Julien
Published: May 2021

It remains unclear whether immune-related adverse events (irAEs) and glucocorticoid use could impact long-term outcomes in patients treated for solid tumors with immune checkpoint inhibitors (ICI). All patients treated with a single-agent ICI for any advanced cancer were included in this retrospective unicentric study. The objectives were to assess the impact of grade ≥3 irAEs, glucocorticoid use and the interruption of immunotherapy on progression-free survival (PFS) and overall survival (OS). In this 828-patient cohort, the first occurrence of grade ≥3 irAEs had no significant impact on PFS or OS. Glucocorticoid administration for the irAEs was associated with a significantly shorter PFS (adjusted HR 3.0; p = 0.00040) and a trend toward shorter OS. ICI interruption was associated with a significantly shorter PFS (adjusted HR 3.5; p < 0.00043) and shorter OS (HR 4.5; p = 0.0027). Glucocorticoid administration and ICI interruption were correlated. In our population of patients treated with single agent ICI, grade ≥3 irAEs did not impact long-term outcomes. However, the need for glucocorticoids and the interruption of immunotherapy resulted in poorer long-term outcomes. The impact of grade ≥3 irAEs reported in other studies might then be explained by the management of the irAEs.