bluuebunny/crossref_metadata_2025 huggingface dataset:
Nadir oxygen delivery is associated with postoperative acute kidney injury in low-weight infants undergoing cardiopulmonary bypass
Authors: Gao Peng , Jin Yu , Zhang Peiyao , Wang Wenting , Hu Jinxiao , Liu Jinping
Published: December 2022

Background

Acute kidney injury (AKI) is common after cardiac surgery with cardiopulmonary bypass (CPB) and is associated with increased mortality and morbidity. Nadir indexed oxygen delivery (DO2i) lower than the critical threshold during CPB is a risk factor for postoperative AKI. The critical DO2i for preventing AKI in children has not been well studied. The study aimed to explore the association between nadir DO2i and postoperative AKI in infant cardiac surgery with CPB.

Methods

From August 2021 to July 2022, 413 low-weight infants (≤10 kg) undergoing cardiac surgery with CPB were consecutively enrolled in this prospective observational study. Nadir DO2i was calculated during the hypothermia and rewarming phases of CPB, respectively. The association between nadir DO2i and postoperative AKI was investigated in mild hypothermia (32–34°C) and moderate hypothermia (26–32°C).

Results

A total of 142 (38.3%) patients developed postoperative AKI. In patients undergoing mild hypothermia during CPB, nadir DO2i in hypothermia and rewarming phases was independently associated with postoperative AKI. The cutoff values of nadir DO2i during hypothermia and rewarming phases were 258 mL/min/m2and 281 mL/min/m2, respectively. There was no significant association between nadir DO2i and postoperative AKI in patients undergoing moderate hypothermia during CPB.

Conclusion

In low-weight infants undergoing mild hypothermia during CPB, the critical DO2i for preventing AKI was 258 mL/min/m2in the hypothermia phase and 281 mL/min/m2for rewarming. Moreover, an individualized critical DO2i threshold should be advocated during CPB.
Case report: Cardiac arrest during carotid body tumor resection indicating carotid sinus hypersensitivity
Authors: Duan Hong Yong , Guan Qiang , Guo Yu Jie , Liang Ning
Published: December 2022

Background

Carotid body tumor surgery is associated with various complications. However, intraoperative cardiac arrest is very rare and no more than 10 cases have been reported.

Case description

A 58-year-old woman diagnosed with bilateral carotid body tumors underwent right carotid body tumor surgery. Sudden cardiac arrest occurred during the resection and was attributed to carotid sinus hypersensitivity. The patient recovered after prompt treatment and the tumor was removed completely with no complications.

Conclusion

Cardiac arrest attributed to carotid sinus hypersensitivity during carotid body tumor resection is very rare. Proper treatments can reverse intraoperative cardiac arrest. If carotid sinus hypersensitivity is detected preoperatively, prophylactic temporary pacemaker implantation may be appropriate.
Four propositions on how to conceptualize, research, and develop teacher educator professionalism
Authors: Vanassche Eline
Published: December 2022

This article offers four propositions on how to conceptualize, research, and develop teacher educator professionalism. These propositions start from one central thesis: teacher educator professionalism can only be fully understood by looking at actual teacher education practices and how these unfold and develop. Based on this conception of enacted professionalism, the first proposition questions the emphasis on teaching experience for teacher educators; the second proposition advances the idea that teacher education cannot be envisioned without someone to educate; the third proposition suggests making the teacher education institute as an organization a central focus of understanding; the fourth proposition addresses the need to reframe traditional forms of knowledge (re)presentation. In conclusion, a framework for future research and practice of teacher educator development is proposed.
Insulin resistance, autophagy and apoptosis in patients with polycystic ovary syndrome: Association with PI3K signaling pathway
Authors: Tong Cheng , Wu Yue , Zhang Lingling , Yu Ying
Published: December 2022

Polycystic ovary syndrome (PCOS) is a disease in which endocrine metabolic abnormalities coexist with reproductive system abnormalities, with the main clinical manifestations including abnormal menstruation, hirsutism, acne, infertility, and obesity, and it is also a high risk for the development of many pregnancy complications, gynecological malignancies and other diseases. Therefore, timely intervention to prevent the progression of PCOS is of great significance for improving the quality of life of most female patients. Insulin resistance (IR) is one of the most common endocrine disorders in PCOS patients, with approximately 75% of PCOS patients experiencing varying degrees of IR. It is now believed that it is mainly related to the PI3K signaling pathway. The role of autophagy and apoptosis of ovarian granulosa cells (GCs) in the pathogenesis of PCOS has also been gradually verified in recent years. Coincidentally, it also seems to be associated with the PI3K signaling pathway. Our aim is to review these relevant studies, to explore the association between the IR, cellular autophagy and apoptosis in PCOS patients and the PI3K pathway. We summarize some of the drug studies that have improved PCOS as well. We have also found that proteomics holds great promise in exploring the pathogenesis of PCOS, and we have published our views on this.
Early postoperative calcitonin-to-preoperative calcitonin ratio as a predictive marker for structural recurrence in sporadic medullary thyroid cancer: A retrospective study
Authors: Jiao Zan , Wu Tong , Jiang Mingjie , Jiang Shuxian , Jiang Ke , Peng Jin , Luo Guangfeng , Yu Yongchao , Chen Weichao , Yang Ankui
Published: December 2022

Background

Calcitonin (Ctn) is widely used as a marker in the diagnosis, prognosis, and postoperative follow-up of patients with medullary thyroid carcinoma (MTC). The prognostic value of postoperative calcitonin-to-preoperative calcitonin ratio (CR), reflecting the change in Ctn level of response to initial treatment, remains uncertain in long-term disease outcomes. This study aims to determine the cut-off value of CR for predicting structural recurrence and assess the prognostic role of CR in patients with MTC.

Methods

We retrospectively reviewed patients with MTC in Sun Yat-sen University Cancer Center (SYSUCC) between 2000 and 2022. CR is defined as the ratio of postoperative Ctn level on the day of discharge divided by preoperative Ctn level. In order to determine the optimal cut-off value of CR, the receiver operating characteristic (ROC) analysis was performed. We evaluate the effect of CR on recurrence-free survival (RFS) by using the Kaplan-Meier method and Cox regression analysis. Then, a nomogram based on CR was constructed.

Results

In total, 112 sporadic MTC patients were included in this study. The optimal cut-off value of CR that predicted disease recurrence was 0.125. Patients with CR≥0.125 showed significantly worse RFS than patients with CR <0.125, respectively (3-years RFS rate of 63.1 vs. 94.7%, 5-years RFS rate of 50.7 vs. 90.3%, P < 0.001). In the multivariate analysis, CR was the strongest independent predictor of structural recurrence (HR: 5.050, 95% CI: 2.247–11.349, P <0.001). Tumor size (HR: 1.321, 95% CI: 1.010–1.726, P =0.042), multifocality (HR: 2.258, 95% CI: 1.008–5.058, P =0.048) and metastasized lymph nodes (HR: 3.793, 95% CI: 1.617–8.897, P <0.001) were also independent predictors of structural recurrence. The uncorrected concordance index (c-index) of the nomogram was 0.827 (95% CI, 0.729-0.925) for RFS, and bias-corrected c-index were similar. As compared to TNM stage, the nomogram based on CR provided better discrimination accuracy.

Conclusions

We demonstrate that CR is a strong prognostic marker to predict structural recurrence in patients with sporadic MTC. The nomogram incorporating CR provided useful prediction of RFS for patients with sporadic MTC to provide personalized treatment.
Achievements and gaps in projection studies on the temperature-attributable health burden: Where should we be headed?
Authors: Rai Masna , Breitner Susanne , Zhang Siqi , Rappold Ana G. , Schneider Alexandra
Published: December 2022

Future projection of the temperature-related health burden, including mortality and hospital admissions, is a growing field of research. These studies aim to provide crucial information for decision-makers considering existing health policies as well as integrating targeted adaptation strategies to evade the health burden. However, this field of research is still overshadowed by large uncertainties. These uncertainties exist to an extent in the future climate and population models used by such studies but largely in the disparities in underlying assumptions. Existing studies differ in the factors incorporated for projection and strategies for considering the future adaptation of the population to temperature. These differences exist to a great degree because of a lack of robust evidence as well as gaps in the field of climate epidemiology that still require extensive input from the research community. This narrative review summarizes the current status of projection studies of temperature-attributable health burden, the guiding assumptions behind them, the common grounds, as well as the differences. Overall, the review aims to highlight existing evidence and knowledge gaps as a basis for designing future studies on temperature-attributable health burden estimation. Finding a robust methodology for projecting the future health burden could be a milestone for climate epidemiologists as this would largely benefit the world when applying this technique to project the climate-attributable cause-specific health burden and adapt our existing health policies accordingly.
Sleeping sites provide new insight into multiple central place foraging strategies of Tibetan macaques (Macaca thibetana)
Authors: Li Bo-Wen , Li Wen-Bo , Xia Dong-Po , Zhang Tong , Yang Pei-Pei , Li Jin-Hua
Published: December 2022

Food resources, including food types, quantity, and quality, are the key factors that determine the survival and reproduction of wild animals. However, the most basic requirement is access to food. The choice of sleeping sites plays a crucial role in efficiently acquiring food and provides a useful starting point for studying foraging strategies. We collected data on sleeping site and foraging patch uses of wild Tibetan macaques (Macaca thibetana) in Huangshan, Anhui, China, from September 2020 to August 2021. We found that Tibetan macaques used 50 different sleeping sites, mostly located on cliffs, some of which they reused. Sleeping site altitude differed significantly according to season, with higher altitudes recorded in summer and winter. Tibetan macaques did not sleep as much as expected in the peripheral regions of their home range. The sleeping sites were often distributed in proximity to foraging patches, and there was a positive correlation between the use of sleeping sites and surrounding foraging patches. The utilization of foraging patches by Tibetan macaques is inclined towards the multiple central place foraging strategy. Our results provide supportive evidence for the proximity to food resource hypothesis and indicate the important role of sleeping sites in food resource utilization in Tibetan macaques.
Refugees' time investments—Differences in the time use of refugees, other immigrants, and natives in Germany
Authors: Kuhlemann Jana
Published: December 2022

Introduction

Since the 2015/16 refugee influx to Germany and other European countries, these host societies have been challenged with the integration of culturally distant refugees. These recent arrivals can strategically invest their time in activities promoting their integration, thereby rendering time use as a channel of integration. Refugees are a vulnerable group that differs from other immigrants with respect to their migration motivation, experience, and conditions in the receiving countries. Accordingly, refugees might also differ from other immigrants with respect to their time use. This might play a role in explaining differences in refugees' and other immigrants' integration outcomes.

Methods

Using a cluster analysis approach, this contribution (1) descriptively examines whether and to what extent refugees' time use differs from that of other immigrants and the host-country population in Germany and (2) examines the role of refugees' legal status for their time use. The study examines time allocation to different activities of refugees, other first-generation immigrants, and native Germans, using data collected from 2016 to 2019 of the German Socio-Economic Panel, including the IAB-BAMF-SOEP Survey of Refugees and the IAB-SOEP Migration Sample.

Results and discussion

Results from (1) the cluster analysis approach show different clusters of time use patterns for the three population groups of refugees, other immigrants, and natives. For native Germans and other immigrants, the dominant time use cluster is characterized by full-time investment in employment activities. For refugees, the dominant time use pattern is characterized by low overall invested hours to the measured activities (low activitycluster). In contrast to the other two groups, a cluster of refugees predominantly allocating their time to employment activities is not found. Pooled analyses (2) of the role of refugees' legal status show some evidence that those who have a form of protection status, in comparison to those who have asylum seeker status, have a lower probability to display childcare- and household-related activities than to reportlow activity. However, fixed effects analyses show that refugees receiving a positive decision on their asylum application do not change with respect to their time use patterns.
Ethnic discrimination in neighborhood ingroup-outgroup encounters: Reducing threat-perception and increasing fairness as possible solutions
Authors: Kleinewiese Julia
Published: December 2022

Research on discriminating behavior against ethnic minorities in everyday situations is still a rather under-researched field, since most prior research on ethnic discrimination focuses on housing markets, job markets, criminal justice, institutions or discourses. This article contributes toward filling the research-gap on everyday discrimination by bringing together prior research from sociology and social-psychology, including threat and competition theories from integration research, social identity theory, particularism-universalism theory and experimental findings on fairness norms. It conceptually advances the field by combining them into an integrated interdisciplinary approach that can examine discriminating behavior in everyday situations. This approach studies the dynamics of ingroup-outgroup relationships, fairness norms and threat in regard to negative behavior toward others (e.g., a neighbor). In particular, it focusses on the dynamics under which negative behavior is more likely toward an ethnic outgroup-person than an ingroup-person (i.e., discriminating behavior). To scrutinize the expectations derived within this framework, a factorial survey experiment was designed, implemented and analyzed (by means of multilevel mixed-effects linear regressions and average marginal effects). The survey experiment presents a hypothetical scenario between two neighbors in order to measure the effects and dynamics of ingroup-outgroup relationships, fairness norms and threat on behavior. While no significant outgroup-effect can be found in the general analysis of the main effects, more in-depth analyses show an interplay of situational cues: Outgroup-discriminating behavior becomes significantly more likely when the “actor” has low general fairness norms and/or when threat-level in a situation is low. These results foreground the importance of interdisciplinary in-depth analyses of dynamics for understanding the conditions under which discriminating behavior takes place in everyday situations—and for deriving measures that can reduce discrimination.
cGAS deficiency enhances inflammasome activation in macrophages and inflammatory pathology in pristane-induced lupus
Authors: Kumpunya Sarinya , Thim-uam Arthid , Thumarat Chisanu , Leelahavanichkul Asada , Kalpongnukul Nuttiya , Chantaravisoot Naphat , Pisitkun Trairak , Pisitkun Prapaporn
Published: December 2022

Introduction

Type I interferon (IFN) plays a vital role in the pathogenesis of systemic lupus erythematosus. Cyclic GMP AMP synthase (cGAS) is a cytosolic DNA sensor that recognizes dsDNA and creates cGAMP to activate STING-mediated type I IFN production. The activation of STING induces lupus disease in Fcgr2b deficient mice through the differentiation of dendritic cells. In contrast, Cgas-deficient mice could be generated more autoantibody production and proteinuria in pristane-induced lupus (PIL). These data suggested that the other dsDNA sensors could be involved in lupus development mechanisms.

Methods

This study aimed to identify the cGAS-mediated mechanisms contributing to lupus pathogenesis in PIL. The Cgas-deficient and WT mice were induced lupus disease with pristane and subsequently analyzed autoantibody, histopathology, and immunophenotypes. The lung tissues were analyzed with the expression profiles by RT-PCR and western blot. The bone marrow-derived macrophages were stimulated with inflammasome activators and observed pyroptosis.

Results

The Cgas-/- mice developed more severe pulmonary hemorrhage and autoantibody production than WT mice. The activated dendritic cells, IFN-g-, and IL-17a-producing T helper cells, and infiltrated macrophages in the lung were detected in Cgas-/- mice higher than in WT mice. We observed an increase in expression of Aim2, Casp11, and Ifi16 in the lung and serum IL-1a but IL-1b in pristane-injected Cgas-/- mice. The rise of Caspase-11 in the lung of pristane-injected Cgas-/- mice suggested noncanonical inflammasome activation. The activation of AIM2 and NLRP3 inflammasomes in bone marrow-derived macrophages (BMDMs) enhanced the number of dead cells in Cgas-/- mice compared with WT mice. Activation of the inflammasome significantly induced pyroptosis in Cgas-/- BMDMs. The dsDNA level, but not mitochondrial DNA, increased dramatically in pristane-injected Cgas-/- mice suggesting the dsDNA could be a ligand activating inflammasomes. The cGAS agonist-induced BMDM activation in the Cgas-/- mice indicated that the activation of DNA sensors other than cGAS enhanced activated macrophages.

Conclusion

These findings suggested that cGAS hampers the unusual noncanonical inflammasome activation through other DNA sensors.
The relationship between chronic immune response and neurodegenerative damage in long COVID-19
Authors: Elizalde-Díaz José Pedro , Miranda-Narváez Clara Leticia , Martínez-Lazcano Juan Carlos , Martínez-Martínez Eduardo
Published: December 2022

In the past two years, the world has faced the pandemic caused by the severe acute respiratory syndrome 2 coronavirus (SARS-CoV-2), which by August of 2022 has infected around 619 million people and caused the death of 6.55 million individuals globally. Although SARS-CoV-2 mainly affects the respiratory tract level, there are several reports, indicating that other organs such as the heart, kidney, pancreas, and brain can also be damaged. A characteristic observed in blood serum samples of patients suffering COVID-19 disease in moderate and severe stages, is a significant increase in proinflammatory cytokines such as interferon-α (IFN-α), interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6) and interleukin-18 (IL-18), as well as the presence of autoantibodies against interferon-α (IFN-α), interferon-λ (IFN-λ), C-C motif chemokine ligand 26 (CCL26), CXC motif chemokine ligand 12 (CXCL12), family with sequence similarity 19 (chemokine (C-C motif)-like) member A4 (FAM19A4), and C-C motif chemokine ligand 1 (CCL1). Interestingly, it has been described that the chronic cytokinemia is related to alterations of blood-brain barrier (BBB) permeability and induction of neurotoxicity. Furthermore, the generation of autoantibodies affects processes such as neurogenesis, neuronal repair, chemotaxis and the optimal microglia function. These observations support the notion that COVID-19 patients who survived the disease present neurological sequelae and neuropsychiatric disorders. The goal of this review is to explore the relationship between inflammatory and humoral immune markers and the major neurological damage manifested in post-COVID-19 patients.
Piperlongumine alleviates corneal allograft rejection via suppressing angiogenesis and inflammation
Authors: Fan Xiangyu , Qiu Jini , Yuan Tianjie , Zhang Jing , Xu Jianjiang
Published: December 2022

Background

Neovascularization and inflammatory response are two essential features of corneal allograft rejection. Here, we investigated the impact of Piperlongumine (PL) on alleviating corneal allograft rejection, primarily focusing on pathological angiogenesis and inflammation.

Methods

A murine corneal allograft transplantation model was utilized to investigate the role of PL in preventing corneal allograft rejection. PL (10 mg/kg) or vehicle was intraperitoneally injected daily into BALB/c recipients from day -3 to day 14. The clinical signs of the corneal grafts were monitored for 30 days. Corneal neovascularization and inflammatory cell infiltration were detected by immunofluorescence staining and immunohistochemistry. The proportion of CD4+ T cells and macrophages in the draining lymph nodes (DLNs) was examined by flow cytometry. In vitro, HUVECs were cultured under hypoxia or incubated with TNF-α to mimic the hypoxic and inflammatory microenvironment favoring neovascularization in corneal allograft rejection. Multiple angiogenic processes including proliferation, migration, invasion and tube formation of HUVECs in hypoxia with or without PL treatment were routinely evaluated. The influence of PL treatment on TNF-α-induced pro-inflammation in HUVECs was investigated by real-time PCR and ELISA.

Results

In vivo, PL treatment effectively attenuated corneal allograft rejection, paralleled by coincident suppression of neovascularization and alleviation of inflammatory response. In vitro, PL distinctively inhibited hypoxia-induced angiogenic processes in HUVECs. Two key players in hypoxia-induced angiogenesis, HIF-1α and VEGF-A were significantly suppressed by PL treatment. Also, TNF-α-induced pro-inflammation in HUVECs was hampered by PL treatment, along with a pronounced reduction in ICAM-1, VCAM-1, CCL2, and CXCL5 expression.

Conclusions

The current study demonstrated that PL could exhibit both anti-angiogenic and anti-inflammatory effects in preventing corneal allograft rejection, highlighting the potential therapeutic applications of PL in clinical strategy.
Density dependent regulation of inflammatory responses in macrophages
Authors: Vaughan-Jackson Alun , Stodolak Szymon , Ebrahimi Kourosh H. , Johnson Errin , Reardon Paul K. , Dupont Maeva , Zhang Shengpan , McCullagh James S. O. , James William S.
Published: December 2022

Macrophage distribution density is tightly regulated within the body, yet the importance of macrophage crowding during in vitro culture is largely unstudied. Using a human induced pluripotent stem cell (iPSC)-derived macrophage model of tissue resident macrophages, we characterize how increasing macrophage culture density changes their morphology and phenotype before and after inflammatory stimulation. In particular, density drives changes in macrophage inflammatory cytokine and chemokine secretion in both resting and activated states. This density regulated inflammatory state is also evident in blood monocyte derived-macrophages, the human monocytic THP-1 immortalized cell line, and iPSC-derived microglia. Density-dependent changes appear to be driven by a transferable soluble factor, yet the precise mechanism remains unknown. Our findings highlight cell plating density as an important but frequently overlooked consideration of in vitro macrophage research relevant to a variety of fields ranging from basic macrophage cell biology to disease studies.
Operational monitoring of water quality with a Do-It-Yourself modular instrument
Authors: Rodero Carlos , Bardaji Raul , Olmedo Estrella , Piera Jaume
Published: December 2022

We analyze the efficacy of using a Do-It-Yourself (DIY) modular instrument to estimate the diffuse attenuation coefficient (Kd) of photosynthetically active radiation (PAR), which can be used for operational oceanography in turbid shallow waters. This parameter evaluates the water transparency, as it summarizes several water components providing an indicator for water quality. Historically, water transparency has been measured with a simple and inexpensive tool: the Secchi disk. Although it contributes a valuable index of visual water clarity, the quality of its measurements is user-dependent, and it does not enable the automatic monitoring of the water quality. For this reason, we need electronic devices to get accurate measures and facilitate long-term evaluations for water quality monitoring. This paper has two main objectives: First, to present the KduPRO, a low-cost and DIY moored instrument. The KduPRO is an evolution of the KdUINO buoy, that provides an estimation of the water transparency in coastal areas and continental waters, with an automatic quality control parameter that makes this sensor suitable for operational observing systems; and second, to provide a replicability analysis associated to the uncertainty of its Kd estimations. This instrument is based on a modular system of light sensors, independent of each other, measuring the irradiance at different depths. This study analyses the performance of the KduPRO with other reference commercial instruments, the performance between different modules of the same system and finally, a case study of measuring the water quality in Loch Leven (a lake in Scotland). The affordable cost, ease of use and measurement repeatability make this instrument a potentially valuable tool for anyone interested in monitoring water quality.
Divulging the social sex change mechanism in a unique model system for studying the sexual plasticity of protogynous hermaphrodite fish, three bamboo leaf wrasse (Pseudolabrus sieboldi)
Authors: Chakraborty Tapas , Yamamoto Yume , Hanai Shoma , Hirano Mayumi , Mohapatra Sipra , Yamaguchi Akihiko , Takeda Tatsusuke , Matsuyama Michiya , Ohta Kohei
Published: December 2022

The gonadal sexual fate of vertebrates is either defined by genetics or environment, or a combination of both factors. Interestingly, in sequential hermaphroditism, the animal can undergo natural sex changes from female-to-male, male-to-female, and bidirectional way throughout their lives. This change exhibits the process which shifts between oogenesis and spermatogenesis and is regarded as an ideal instance of sexual plasticity. To develop the experimental model for studying the sexual plasticity of protogynous fish, the social conditions that induce sex changes were defined in wrasse, Pseudolabrus sieboldi. When six females were kept together in a tank, the largest female became a male, whereas a similar conversion did not occur when only two females were present in a tank. A semi-gonadectomy analysis developed in the present study verified the direct relationship between gonadal sex and body coloration. In P. sieboldi, the sex change is controlled by the relative body size of an individual within a group, rather than by absolute body size. When six females were kept in smaller sized tanks, delayed sex change or unchanged individuals was observed. Overall, more than 90% of the largest females demonstrated sex change after being housed with five smaller females in different sizes of tanks ranging from 80 to 500 L. Furthermore, the experiment using a transparent barrier suggested that visual stimuli are one of the major cues to initiate sex change. Our findings on the laboratory conditions leading to the initiation of sex change in wrasse suggest the usefulness of this species as a model organism for comparative studies in molecular, cellular, and physiological mechanisms of sexual plasticity, as well as on social and reproductive behaviors.
Influence of the interannual variability of the Kuroshio Extension on the Mediterranean trough in the cold season
Authors: Jiang Yuxi , Sun Jianxiang , Ma Li , Li Huan , Wang Yanshuo
Published: December 2022

The impacts of the Kuroshio Extension (KE) interannual fluctuation on the Mediterranean trough (MedT) and associated Euro-Mediterranean climate in wintertime are analyzed in this study. It appears that when the KE index is positive (corresponds to a stable KE state with sharp sea surface temperature front and subsided oceanic eddies), the MedT is observed to be stronger in the west of its climatological position, resulting in a colder condition to the west of the Black Sea and more precipitation in the East Mediterranean and Anatolia Plateau. The opposite atmospheric pattern holds when the KE index is negative (unstable KE state). The empirical orthogonal function analysis is performed on 500-hPa geopotential height to obtain the dominant modes of MedT. The results show the KE index is significantly correlated with the intensity and zonal displacement of MedT, with correlation coefficients of 0.57 and 0.40, respectively. The low-frequency Rossby wave activity and high-frequency eddies are revealed as the prominent contributors to this co-variability between the KE and MedT. The upward turbulent heat flux transport from the ocean to the atmosphere strengthened by the stable KE activates the Rossby waves propagating upward and westward to the MedT region, contributing to approximately 30% of the MedT deepening. Meanwhile, the KE-driven Rossby waves intensify the background baroclinicity to the north of the Mediterranean Sea and thus enhance the transient eddy activity, consequently leading to another 20% of the MedT amplification via the eddy–mean flow interaction. The findings highlight the impacts of the KE state on the meteorological conditions in the Euro-Mediterranean region, which can potentially optimize the forecast accuracies of the MedT and associated Euro-Mediterranean climate variability.
Ultrasound cyclo-plasty for moderate glaucoma: Eighteen-month results from a prospective study
Authors: Wang Rui-Xue , Li Ning , Chen Xiao-Ya
Published: December 2022

Purpose

To evaluate the long-term clinical efficacy of ultrasound cyclo-plasty (UCP) in the treatment of moderate glaucoma and molecular effects in animal experiments.

Methods

An 18-month clinical study was conducted among 32 patients with moderate glaucoma. The primary outcome was surgical success, defined as a reduction in intraocular pressure (IOP) of greater than or equal to 20% from the baseline and an IOP value of greater than 5 mmHg at the last follow-up. The secondary outcomes were related to the quality of life, complications, and mean IOP value at each follow-up. In the animal experiment, 20 New Zealand rabbits were used to establish a high-IOP model and implement UCP. The distribution of aquaporin 4 (AQP4) in the ciliary body and the tissue changes under electron microscopy were observed after surgery.

Results

The mean patient IOP decreased from 34.9 ± 4.9 mmHg before surgery to 23.5 ± 5.2 mmHg at 18 months after UCP. No vision loss occurred in any patient. Some patients had postoperative complications, but the symptoms were mild and disappeared within 3 months after the surgery. Most patients had good postoperative quality of life. Histology showed that AQP4 remained in the ciliary muscle after UCP, and only the bilayered epithelial cells showed coagulative necrosis. Furthermore, electron microscopic observation revealed the destruction of ciliary process cells covered by ultrasound after UCP.

Conclusion

UCP is associated with mild postoperative reactions and the mild treatment of ciliary tissue and is a safe and effective method for reducing IOP in moderate glaucoma.
The training of wrist arthroscopy
Authors: Shi Haifei , Lu Pan , Yu Dongdong , Wang Jiwen , Wang Zhenhua , Zhuang Baotang , Shao Chao , Liu Chang , Liu Bo
Published: December 2022

The wrist is a complex joint that bridges the hand to the forearm. Patients with wrist disorders increasingly prefer minimally invasive procedures for wrist joint diagnosis and treatment. Wrist arthroscopy offers direct visualization of the structures of the joint anatomy and existing disease processes while causing minimal damage to surrounding soft tissue. However, it requires a high level of technical ability for wrist arthroscopy practitioners. Therefore, an improved focus on wrist arthroscopy training combining new educational media and traditional practice should aid in the development of novel wrist arthroscopy training mode. This article aims to describe the status of wrist training and evaluation systems and introduce a new progressive wrist training system.
Coronavirus disease 2019 (COVID-19) excess mortality outcomes associated with pandemic effects study (COPES): A systematic review and meta-analysis
Authors: Lu David , Dhanoa Sumeet , Cheema Harleen , Lewis Kimberley , Geeraert Patrick , Merrick Benjamin , Vander Leek Aaron , Sebastianski Meghan , Kula Brittany , Chaudhuri Dipayan , Basmaji John , Agrawal Arnav , Niven Dan , Fiest Kirsten , Stelfox Henry T. , Zuege Danny J. , Rewa Oleksa G. , Bagshaw Sean M. , Lau Vincent I.
Published: December 2022

Background and aim

With the Coronavirus Disease 2019 (COVID-19) pandemic continuing to impact healthcare systems around the world, healthcare providers are attempting to balance resources devoted to COVID-19 patients while minimizing excess mortality overall (both COVID-19 and non-COVID-19 patients). To this end, we conducted a systematic review (SR) to describe the effect of the COVID-19 pandemic on all-cause excess mortality (COVID-19 and non-COVID-19) during the pandemic timeframe compared to non-pandemic times.

Methods

We searched EMBASE, Cochrane Database of SRs, MEDLINE, Cumulative Index to Nursing and Allied Health Literature (CINAHL) and Cochrane Controlled Trials Register (CENTRAL), from inception (1948) to December 31, 2020. We used a two-stage review process to screen/extract data. We assessed risk of bias using Newcastle-Ottawa Scale (NOS). We used Critical Appraisal and Grading of Recommendations Assessment, Development and Evaluation (GRADE) methodology.

Results

Of 11,581 citations, 194 studies met eligibility. Of these studies, 31 had mortality comparisons (n = 433,196,345 participants). Compared to pre-pandemic times, during the COVID-19 pandemic, our meta-analysis demonstrated that COVID-19 mortality had an increased risk difference (RD) of 0.06% (95% CI: 0.06–0.06% p < 0.00001). All-cause mortality also increased [relative risk (RR): 1.53, 95% confidence interval (CI): 1.38–1.70, p < 0.00001] alongside non-COVID-19 mortality (RR: 1.18, 1.07–1.30, p < 0.00001). There was “very low” certainty of evidence through GRADE assessment for all outcomes studied, demonstrating the evidence as uncertain.

Interpretation

The COVID-19 pandemic may have caused significant increases in all-cause excess mortality, greater than those accounted for by increases due to COVID-19 mortality alone, although the evidence is uncertain.

Systematic review registration

[https://www.crd.york.ac.uk/prospero/#recordDetails], identifier [CRD42020201256].
Present and future of gait assessment in clinical practice: Towards the application of novel trends and technologies
Authors: Hulleck Abdul Aziz , Menoth Mohan Dhanya , Abdallah Nada , El Rich Marwan , Khalaf Kinda
Published: December 2022

Background

Despite being available for more than three decades, quantitative gait analysis remains largely associated with research institutions and not well leveraged in clinical settings. This is mostly due to the high cost/cumbersome equipment and complex protocols and data management/analysis associated with traditional gait labs, as well as the diverse training/experience and preference of clinical teams. Observational gait and qualitative scales continue to be predominantly used in clinics despite evidence of less efficacy of quantifying gait.

Research objective

This study provides a scoping review of the status of clinical gait assessment, including shedding light on common gait pathologies, clinical parameters, indices, and scales. We also highlight novel state-of-the-art gait characterization and analysis approaches and the integration of commercially available wearable tools and technology and AI-driven computational platforms.

Methods

A comprehensive literature search was conducted within PubMed, Web of Science, Medline, and ScienceDirect for all articles published until December 2021 using a set of keywords, including normal and pathological gait, gait parameters, gait assessment, gait analysis, wearable systems, inertial measurement units, accelerometer, gyroscope, magnetometer, insole sensors, electromyography sensors. Original articles that met the selection criteria were included.

Results and significance

Clinical gait analysis remains highly observational and is hence subjective and largely influenced by the observer's background and experience. Quantitative Instrumented gait analysis (IGA) has the capability of providing clinicians with accurate and reliable gait data for diagnosis and monitoring but is limited in clinical applicability mainly due to logistics. Rapidly emerging smart wearable technology, multi-modality, and sensor fusion approaches, as well as AI-driven computational platforms are increasingly commanding greater attention in gait assessment. These tools promise a paradigm shift in the quantification of gait in the clinic and beyond. On the other hand, standardization of clinical protocols and ensuring their feasibility to map the complex features of human gait and represent them meaningfully remain critical challenges.
Phage family classification under Caudoviricetes: A review of current tools using the latest ICTV classification framework
Authors: Zhu Yilin , Shang Jiayu , Peng Cheng , Sun Yanni
Published: December 2022

Bacteriophages, which are viruses infecting bacteria, are the most ubiquitous and diverse entities in the biosphere. There is accumulating evidence revealing their important roles in shaping the structure of various microbiomes. Thanks to (viral) metagenomic sequencing, a large number of new bacteriophages have been discovered. However, lacking a standard and automatic virus classification pipeline, the taxonomic characterization of new viruses seriously lag behind the sequencing efforts. In particular, according to the latest version of ICTV, several large phage families in the previous classification system are removed. Therefore, a comprehensive review and comparison of taxonomic classification tools under the new standard are needed to establish the state-of-the-art. In this work, we retrained and tested four recently published tools on newly labeled databases. We demonstrated their utilities and tested them on multiple datasets, including the RefSeq, short contigs, simulated metagenomic datasets, and low-similarity datasets. This study provides a comprehensive review of phage family classification in different scenarios and a practical guidance for choosing appropriate taxonomic classification pipelines. To our best knowledge, this is the first review conducted under the new ICTV classification framework. The results show that the new family classification framework overall leads to better conserved groups and thus makes family-level classification more feasible.
The spring-like effect of microRNA-31 in balancing inflammatory and regenerative responses in colitis
Authors: Qu Jing , Shao Chunlei , Ying Yongfa , Wu Yuning , Liu Wen , Tian Yuhua , Yin Zhiyong , Li Xiang , Yu Zhengquan , Shuai Jianwei
Published: December 2022

Inflammatory bowel diseases (IBDs) are chronic inflammatory disorders caused by the disruption of immune tolerance to the gut microbiota. MicroRNA-31 (MIR31) has been proven to be up-regulated in intestinal tissues from patients with IBDs and colitis-associated neoplasias. While the functional role of MIR31 in colitis and related diseases remain elusive. Combining mathematical modeling and experimental analysis, we systematically explored the regulatory mechanism of MIR31 in inflammatory and epithelial regeneration responses in colitis. Level of MIR31 presents an “adaptation” behavior in dextran sulfate sodium (DSS)-induced colitis, and the similar behavior is also observed for the key cytokines of p65 and STAT3. Simulation analysis predicts MIR31 suppresses the activation of p65 and STAT3 but accelerates the recovery of epithelia in colitis, which are validated by our experimental observations. Further analysis reveals that the number of proliferative epithelial cells, which characterizes the inflammatory process and the recovery of epithelia in colitis, is mainly determined by the inhibition of MIR31 on IL17RA. MIR31 promotes epithelial regeneration in low levels of DSS-induced colitis but inhibits inflammation with high DSS levels, which is dominated by the competition for MIR31 to either inhibit inflammation or promote epithelial regeneration by binding to different targets. The binding probability determines the functional transformation of MIR31, but the functional strength is determined by MIR31 levels. Thus, the role of MIR31 in the inflammatory response can be described as the “spring-like effect,” where DSS, MIR31 action strength, and proliferative epithelial cell number are regarded as external force, intrinsic spring force, and spring length, respectively. Overall, our study uncovers the vital roles of MIR31 in balancing inflammation and the recovery of epithelia in colitis, providing potential clues for the development of therapeutic targets in drug design.
Characterization of cellular senescence patterns predicts the prognosis and therapeutic response of hepatocellular carcinoma
Authors: Tang Yuqin , Guo Chengbin , Chen Chuanliang , Zhang Yongqiang
Published: December 2022

Background: Hepatocellular carcinoma (HCC) is a prevalent malignancy with a high mortality rate. Cellular senescence, an irreversible state of cell cycle arrest, plays a paradoxical role in cancer progression. Here, we aimed to identify Hepatocellular carcinoma subtypes by cellular senescence-related genes (CSGs) and to construct a cellular senescence-related gene subtype predictor as well as a novel prognostic scoring system, which was expected to predict clinical outcomes and therapeutic response of Hepatocellular carcinoma.

Methods: RNA-seq data and clinical information of Hepatocellular carcinoma patients were derived from The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC). The “multi-split” selection was used to screen the robust prognostic cellular senescence-related genes. Unsupervised clustering was performed to identify CSGs-related subtypes and a discriminant model was obtained through multiple statistical approaches. A CSGs-based prognostic model-CSGscore, was constructed by LASSO-Cox regression and stepwise regression. Immunophenoscore (IPS) and Tumor Immune Dysfunction and Exclusion (TIDE) were utilized to evaluate the immunotherapy response. Tumor stemness indices mRNAsi and mDNAsi were used to analyze the relationship between CSGscore and stemness.

Results: 238 robust prognostic differentially expressed cellular senescence-related genes (DECSGs) were used to categorize all 336 hepatocellular carcinoma patients of the TCGA-LIHC cohort into two groups with different survival. Two hub genes, TOP2A and KIF11 were confirmed as key indicators and were used to form a precise and concise cellular senescence-related gene subtype predictor. Five genes (PSRC1, SOCS2, TMEM45A, CCT5, and STC2) were selected from the TCGA training dataset to construct the prognostic CSGscore signature, which could precisely predict the prognosis of hepatocellular carcinoma patients both in the training and validation datasets. Multivariate analysis verified it as an independent prognostic factor. Besides, CSGscore was also a valuable predictor of therapeutic responses in hepatocellular carcinoma. More downstream analysis revealed the signature genes were significantly associated with stemness and tumor progression.

Conclusion: Two subtypes with divergent outcomes were identified by prognostic cellular senescence-related genes and based on that, a subtype indicator was established. Moreover, a prognostic CSGscore system was constructed to predict the survival outcomes and sensitivity of therapeutic responses in hepatocellular carcinoma, providing novel insight into hepatocellular carcinoma biomarkers investigation and design of tailored treatments depending on the molecular characteristics of individual patients.
Feature optimization based on improved novel global harmony search algorithm for motor imagery electroencephalogram classification
Authors: Shi Bin , Chen Xiaokai , Yue Zan , Zeng Feixiang , Yin Shuai , Wang Benguo , Wang Jing
Published: December 2022

Background

Effectively decoding electroencephalogram (EEG) pattern for specific mental tasks is a crucial topic in the development of brain-computer interface (BCI). Extracting common spatial pattern (CSP) features from motor imagery EEG signals is often highly dependent on the selection of frequency band and time interval. Therefore, optimizing frequency band and time interval would contribute to effective feature extraction and accurate EEG decoding.

Objective

This study proposes an approach based on an improved novel global harmony search (INGHS) to optimize frequency-time parameters for effective CSP feature extraction.

Methods

The INGHS algorithm is applied to find the optimal frequency band and temporal interval. The linear discriminant analysis and support vector machine are used for EEG pattern decoding. Extensive experimental studies are conducted on three EEG datasets to assess the effectiveness of our proposed method.

Results

The average test accuracy obtained by the time-frequency parameters selected by the proposed INGHS method is slightly better than artificial bee colony (ABC) and particle swarm optimization (PSO) algorithms. Furthermore, the INGHS algorithm is superior to PSO and ABC in running time.

Conclusion

These superior experimental results demonstrate that the optimal frequency band and time interval selected by the INGHS algorithm could significantly improve the decoding accuracy compared with the traditional CSP method. This method has a potential to improve the performance of MI-based BCI systems.