bluuebunny/crossref_metadata_2025 huggingface dataset:
Progressive Neuropsychiatric Symptoms Following Recurrent COVID-19 Infections in a Previously Healthy Adolescent
Authors: Shawl Christopher , Clark R. Hunter , Edwards Matthew T. , Berson Casey , Zappitelli Megan
Published: November 2023

This is the almost 2-year-long course of a 16-year-old male without significant psychiatry history who abruptly developed symptoms of obsessive-compulsive disorder (OCD) and psychosis following a confirmed coronavirus disease 2019 (COVID-19) infection. His symptoms worsened following a confirmed reinfection with COVID-19. He responded poorly to treatment with selective serotonin reuptake inhibitors, antipsychotics, and benzodiazepines. This case highlights an emerging phenomenon of post-COVID-19 neuropsychiatric sequelae and presents a complicated diagnostic and treatment challenge. The differential for this patient was explored and outlined in detail, and the medical workup recommendations for new-onset mental status changes were reviewed as they pertain to the patient’s assessment and treatment course. While there are several case reports of adolescents with abrupt-onset OCD and psychosis symptoms following COVID-19 infections, none of these reports include worsening of symptoms following reinfection, and few reports follow patients beyond initial hospitalization and treatment.
Risk of Neurodegenerative Diseases in Elderly Koreans with an Initial Diagnosis of Type 2 Diabetes: A Nationwide Retrospective Cohort Study
Authors: Kim Hee-Cheol , Lee Ho-Jun , Kim Yang-Tae , Jang Byeong-Churl
Published: November 2023

Type 2 diabetes (T2D) and neurodegenerative diseases (NDs) are common among elderly individuals. Growing evidence has indicated a strong link between T2D and NDs, such as Alzheimer’s disease. However, previous studies have limitations in exploring the epidemiological relationship among these diseases as a group of NDs rather than as a specific type of ND. We aimed to investigate the risk of NDs in elderly Koreans who were first diagnosed with T2D and determine the association between T2D and NDs. We conducted a retrospective longitudinal cohort study of patients with who were initially diagnosed with T2D using the Korean National Health Information Database. The study participants were categorized into a T2D group (

n
=
155,459

) and a control group (

n
=
155,459

), aged 60–84 years, that were matched for age, sex, and comorbidities. We followed the participants for 10 years to investigate the incidence of NDs. The Cox proportional hazards regression model was used to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs) for NDs. The numbers of patients diagnosed with ND at the end of follow-up were as follows: 51,096/155,459 (32.9%) in the T2D group and 44,673/155,459 (28.7%) in the control group (

χ

2

=
622.53

,

p
<
0.001

). The incidences of NDs in the T2D and control groups were 44.68 (95% CI: 44.29, 45.07) and 36.89 (95% CI: 36.55, 37.24) cases per 1,000 person-years at risk, respectively. The overall incidence of NDs was higher in the T2D group than that in the control group (HR: 1.23, 95% CI: 1.22, 1.25,

p
<
0.001

). This study revealed a higher incidence of NDs in elderly Koreans who were initially diagnosed with T2D. This suggests that T2D is a risk factor for NDs in elderly Koreans.
Association between Dietary Anthocyanidins and Biliary Cancer Risk in 98,458 Participants: Results from a Prospective Study
Authors: Xiang Ling , Wu Dabin , Xu Zhiquan , Tang Yunhao , He Hongmei , Wang Yaxu , Gu Haitao , Peng Linglong
Published: November 2023

Background:

Previous studies have suggested anthocyanidins or anthocyanidin-rich foods and extracts exhibit protective effects against various cancers. However, the relationship between dietary anthocyanidins and the risk of biliary cancer remains uncertain.

Methods:

This study used data from the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial to investigate the relationship between total anthocyanidins intake and biliary cancer incidence. Cox regression analysis was conducted to estimate HRs and corresponding 95% confidence intervals (CI) for the incidence of biliary cancer, with adjustments made for confounding factors. A restricted cubic spline model was employed to examine the dose–response relationship. In addition, subgroup and sensitivity analyses were conducted to evaluate potential interactions and test the model's robustness.

Results:

During 8.9 years and 872,645.3 person-years of follow-up, 95 cases of biliary cancer were observed. The incidence rate of biliary cancer in this study was 11 cases per 100,000 person-years. Using the fully adjusted Cox regression model, the inverse association was observed between total anthocyanidins intake and the risk of biliary cancer (HR Q4 vs..Q1: 0.52; 95% CI: 0.29–0.91; Ptrend = 0.043). This association remained significant in sensitivity analyses. A linear dose–response relationship (Pnonlinearity = 0.118) and potential interaction with drinking status (Pinteraction = 0.033) were identified.

Conclusions:

This study provides evidence of an inverse association between total anthocyanidins intake and biliary cancer incidence.

Impact:

Our study found a total anthocyanidin-rich diet was associated with a reduced risk of biliary cancer in Americans ages 55 to 74 years.
Elucidating the Heterogeneity of Immunotherapy Response and Immune-Related Toxicities by Longitudinal ctDNA and Immune Cell Compartment Tracking in Lung Cancer
Authors: Murray Joseph C. , Sivapalan Lavanya , Hummelink Karlijn , Balan Archana , White James R. , Niknafs Noushin , Rhymee Lamia , Pereira Gavin , Rao Nisha , Weksler Benny , Bahary Nathan , Phallen Jillian , Leal Alessandro , Bartlett David L. , Marrone Kristen A. , Naidoo Jarushka , Goel Akul , Levy Benjamin , Rosner Samuel , Hann Christine L. , Scott Susan C. , Feliciano Josephine , Lam Vincent K. , Ettinger David S. , Li Qing Kay , Illei Peter B. , Monkhorst Kim , Scharpf Robert B. , Brahmer Julie R. , Velculescu Victor E. , Zaidi Ali H. , Forde Patrick M. , Anagnostou Valsamo
Published: November 2023

Purpose:

Although immunotherapy is the mainstay of therapy for advanced non–small cell lung cancer (NSCLC), robust biomarkers of clinical response are lacking. The heterogeneity of clinical responses together with the limited value of radiographic response assessments to timely and accurately predict therapeutic effect—especially in the setting of stable disease—calls for the development of molecularly informed real-time minimally invasive approaches. In addition to capturing tumor regression, liquid biopsies may be informative in capturing immune-related adverse events (irAE).

Experimental Design:

We investigated longitudinal changes in circulating tumor DNA (ctDNA) in patients with metastatic NSCLC who received immunotherapy-based regimens. Using ctDNA targeted error-correction sequencing together with matched sequencing of white blood cells and tumor tissue, we tracked serial changes in cell-free tumor load (cfTL) and determined molecular response. Peripheral T-cell repertoire dynamics were serially assessed and evaluated together with plasma protein expression profiles.

Results:

Molecular response, defined as complete clearance of cfTL, was significantly associated with progression-free (log-rank P = 0.0003) and overall survival (log-rank P = 0.01) and was particularly informative in capturing differential survival outcomes among patients with radiographically stable disease. For patients who developed irAEs, on-treatment peripheral blood T-cell repertoire reshaping, assessed by significant T-cell receptor (TCR) clonotypic expansions and regressions, was identified on average 5 months prior to clinical diagnosis of an irAE.

Conclusions:

Molecular responses assist with the interpretation of heterogeneous clinical responses, especially for patients with stable disease. Our complementary assessment of the peripheral tumor and immune compartments provides an approach for monitoring of clinical benefits and irAEs during immunotherapy.
Supplementary Figures and Tables from MinimuMM-seq: Genome Sequencing of Circulating Tumor Cells for Minimally Invasive Molecular Characterization of Multiple Myeloma Pathology
Authors: Dutta Ankit K. , Alberge Jean-Baptiste , Lightbody Elizabeth D. , Boehner Cody J. , Dunford Andrew , Sklavenitis-Pistofidis Romanos , Mouhieddine Tarek H. , Cowan Annie N. , Su Nang Kham , Horowitz Erica M. , Barr Hadley , Hevenor Laura , Beckwith Jenna B. , Perry Jacqueline , Cao Amanda , Lin Ziao , Kuhr Frank K. , Mastro Richard G. Del , Nadeem Omar , Greipp Patricia T. , Stewart Chip , Auclair Daniel , Getz Gad , Ghobrial Irene M.
Published: November 2023

Supplementary data contains additional Materials and Methods used to generate results presented only in the supplementary figures and tables, as well as more detailed bioinformatics methods.
Figure S1 shows correlation between clinical measures of disease pathology, survival, and circulating tumor cells enumeration.
Figure S2 shows characteristics of isolated circulating tumor cells from peripheral blood of precursor disease patients.
Figure S3 shows cohort-level genomic characterization of tumor in MM precursor stages with CTCs.
Figure S4 shows longitudinal and tissue-matched genomic characterization of driver mutations.
Figure S5 shows comparison of mutational processes between BMPCs and CTCs assigned to most likely PCAWG composite reference signature.
Table S1 shows clinical characteristics and sampling of participants in this study.
Table S2 shows whole-genome sequencing coverage and library metrics.
Table S3 shows clinical BM FISH results and cells recovered for cohort with matched samples.
Table S4 shows comparison of BCR sequence between BMPCs and CTCs.
Table S5 shows clinical BM FISH results of peripheral blood only cohort and CTCs recovered.
Table S6 shows enumeration of single nucleotide variants and short insertions and deletions discovered from WGS of CTCs.
Table S7 shows enumeration of structural variants reconstructed from WGS of CTCs.
Table S8 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Table S8 shows the results of linear regression analysis: association between ORF1p IgG titers and individual cancer types (stages 1-2) relative to healthy subjects after adjustment for age.
Table S5 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Table S5 shows the distribution of age between cancer and healthy subjects.
Table S4 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Table S4 shows the number of samples for five cancer types and distribution by disease stages.
Table S3 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Table S3 shows IgG titers against ORF1p-derived peptides in healthy persons, lung, and ovarian cancer patients.
Table S2 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Table S2 shows anti-ORF1p IgG titers and detection of ORF1p by Western blots in a set of selected blood samples of cancer patients.
Table S11 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Table S11 shows comparison of anti-ORF1p IgG titers between indicated groups of subjects.
Table S1 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Table S1 contains the list of synthetic peptides derived from ORF1p.
Supplementary Methods from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Methods include synthesis and purification of recombinant L1 antigens, immunoassays for L1 antigens and peptides derived from ORF1p and ORF2p,and Western immunoblotting protocols
Figure S1 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Figure S1 shows characteristics of HeLa cells with tetracycline-inducible L1 retrotransposition reporter.
Fig S9 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Figure S9 shows comparison of anti-ORF1p IgG titers in cancer patients with or without exposure to anti‐cancer therapies.
Fig S8 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Figure S8 shows comparison of anti‐ORF1p IgG titers cancer patients with different tobacco smoking history.
Fig S7 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Figure S7 shows the ROC curves indicating specificity and sensitivity of anti‐ORF1p immunoassay for 5 cancer types.
Fig S6 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Figure S6 shows correlation of anti‐ORF1p and anti‐ORF2p IgG titers in patients with five cancer types and healthy individuals.
Fig S5 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Figure S5 shows anti‐ORF2p IgG titers in the blood of esophageal, lung, pancreatic, ovarian and liver cancer patients.
Fig S4 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Figure S4 demonstrates detection of human ORF1p antigen by sandwich ELISA in the presence of human anti‐ORF1p-reactive antibodies.
Fig S3 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Figure S3 shows immunofluorescent staining and Western immunoblotting of lysates of HeLa cells with inducible L1 expression.
Fig S11 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Supplementary Figure S11 shows comparison of anti‐ORF1p, anti‐ORF2p and anti‐p53 IgG in blood samples from patients with SLE and healthy individuals.
FIGURE 3 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Authors: Vylegzhanina Alexandra V. , Bespalov Ivan A. , Novototskaya-Vlasova Ksenia A. , Hall Brandon M. , Gleiberman Anatoli S. , Yu Han , Leontieva Olga V. , Leonova Katerina I. , Kurnasov Oleg V. , Osterman Andrei L. , Dy Grace K. , Komissarov Alexey A. , Vasilieva Elena , Gehlhausen Jeff , Iwasaki Akiko , Ambrosone Christine B. , Tsuji Takemasa , Matsuzaki Junko , Odunsi Kunle , Andrianova Ekaterina L. , Gudkov Andrei V.
Published: November 2023

Anti-ORF1p IgG response in serum samples from patients with five cancer types. A, Selection of cancers for analysis in anti-ORF1p immunoassay. Sector sizes are proportional to the incidence of these cancers in the United States in 2021 (https://seer.cancer.gov/statfacts/). Color code indicates a 5-year survival. “S” stands for the existence of approved cancer screening protocols. B, Detection of anti-ORF1p IgG titers in serum samples of patients with the indicated cancer types of all stages. Sample size: ovary (N = 979), esophagus (N = 377), lung (N = 907), pancreas (N = 124), liver (N = 217) cancers and healthy (N = 352). C, The same as panel B; only samples from patients with cancer stages 1 and 2 are shown. Sample size: ovary (N = 193), esophagus (N = 79), lung (N = 90), pancreas (N = 40), and liver (N = 67) cancers and healthy (N = 352). The gray areas in B and C mark samples below the anti-ORF1p IgG titer threshold of 450. Statistics were calculated by Dunn multiple comparison test with adjusted P value for anti-ORF1p IgG titers. All P value less than 0.05 is considered significant. D, Anti-ORF1p immunoassay threshold determination. E, Spearman r correlation analysis of anti-ORF1p IgG titers and age among healthy individuals (20–89 years of age) or patients with cancer (18–95 years of age).
Secondary Colo-Colic Intussusception Caused by Giant Polyp of Sigmoid Colon in a 5-Year-Old Girl
Authors: Haizhi Tan , Xiaobing Xiao , Jianrong Deng
Published: November 2023

Intussusception is common in infants and young children. The most common type is small intestinal intussusception or ileo-colic intussusception. Colonic intussusception is rare. Intestinal polyps, especially large polyps or multiple polyps, can cause intussusception. Here, we report a rare case of colo-colic intussusception caused by a giant juvenile polyp of sigmoid colon, and the patient achieved good clinical effect through polypectomy under colonoscopy. So, when children have colonic intussusception, the possibility of colonic polyps should be considered.
Bone Mineral Parameters in Peritoneal Dialysis Patients after Lowering Calcium Concentration in Dialysis Fluids: A Case Series in Patients Using Icodextrin
Authors: Verschuur Lara C. , Liefting Anouschka G. , van Dam Bastiaan , Penne Erik L. , Frerichs Fenneke C.
Published: November 2023

In patients treated with peritoneal dialysis (PD), lowering the calcium level in PD fluids results in lower serum calcium levels and higher parathyroid hormone (PTH) levels. It is hypothesized that this effect is attenuated when patients are using icodextrin 7.5% for the once-daily long dwell (containing high calcium concentration). In this case series, we included 8 stable PD patients (mean age 68 ± 13 years, 7 male), all using icodextrin 7.5% (containing 1.75 mmol/L calcium) for the once-daily long dwell. The calcium content of the PD fluids for the remaining dwells was lowered from 1.75 mmol/L to 1.25 mmol/L. Bone mineral parameters and phosphate prescription at baseline, 6 weeks after this change, and after 6 months were compared. After lowering calcium concentration of the PD fluids – except for the icodextrin 7.5% – from 1.75 mmol/L to 1.25 mmol/L, calcium levels changed from 2.32 ± 0.11 to 2.29 ± 0.12 (p = NS); intact PTH (iPTH) from 39.6 ± 28.3 to 64.9 ± 34.5 pmol/L (p = 0.045); and alkaline phosphatase from 104.13 ± 48.75 to 101.38 ± 32.39 (p = NS). After 6 months, all bone mineral parameters were similar to baseline levels; however, slightly higher calcium-based phosphate binders were prescribed. Lowering calcium content from 1.75 mmol/L to 1.25 mmol/L in PD fluids in patients on icodextrin resulted in stable calcium values, a temporal increase in iPTH and a modest increase in calcium-based phosphate binder prescription. Using icodextrin for the long once-daily dwell appears to attenuate the effects on bone mineral parameters when lowering the calcium concentration of the short dwells.
Polycystic Ovary Syndrome Fuels Cardiovascular Inflammation and Aggravates Ischemic Cardiac Injury
Authors: Gao Ling , Zhao Yichao , Wu Haiyan , Lin Xianhua , Guo Fei , Li Jie , Long Yuhang , Zhou Bokang , She Junsen , Zhang Chen , Sheng Jianzhong , Jin Li , Wu Yanting , Huang Hefeng
Published: November 2023

BACKGROUND:

Reducing cardiovascular disease burden among women remains challenging. Epidemiologic studies have indicated that polycystic ovary syndrome (PCOS), the most common endocrine disease in women of reproductive age, is associated with an increased prevalence and extent of coronary artery disease. However, the mechanism through which PCOS affects cardiac health in women remains unclear.

METHODS:

Prenatal anti-Müllerian hormone treatment or peripubertal letrozole infusion was used to establish mouse models of PCOS. RNA sequencing was performed to determine global transcriptomic changes in the hearts of PCOS mice. Flow cytometry and immunofluorescence staining were performed to detect myocardial macrophage accumulation in multiple PCOS models. Parabiosis models, cell-tracking experiments, and in vivo gene silencing approaches were used to explore the mechanisms underlying increased macrophage infiltration in PCOS mouse hearts. Permanent coronary ligation was performed to establish myocardial infarction (MI). Histologic analysis and small-animal imaging modalities (eg, magnetic resonance imaging and echocardiography) were performed to evaluate the effects of PCOS on injury after MI. Women with PCOS and control participants (n=200) were recruited to confirm findings observed in animal models.

RESULTS:

Transcriptomic profiling and immunostaining revealed that hearts from PCOS mice were characterized by increased macrophage accumulation. Parabiosis studies revealed that monocyte-derived macrophages were significantly increased in the hearts of PCOS mice because of enhanced circulating Ly6C
+
monocyte supply. Compared with control mice, PCOS mice showed a significant increase in splenic Ly6C
+
monocyte output, associated with elevated hematopoietic progenitors in the spleen and sympathetic tone. Plasma norepinephrine (a sympathetic neurotransmitter) levels and spleen size were consistently increased in women with PCOS when compared with those in control participants, and norepinephrine levels were significantly correlated with circulating CD14
++
CD16
−
monocyte counts. Compared with animals without PCOS, PCOS animals showed significantly exacerbated atherosclerotic plaque development and post-MI cardiac remodeling. Conditional
Vcam1
silencing in PCOS mice significantly suppressed cardiac inflammation and improved cardiac injury after MI.

CONCLUSIONS:

Our data documented previously unrecognized mechanisms through which PCOS could affect cardiovascular health in women. PCOS may promote myocardial macrophage accumulation and post-MI cardiac remodeling because of augmented splenic myelopoiesis.
The Population Exchange and Politics in the Historiography of Turkey and Greece
Authors: Lamprou Alexandros
Published: November 2023

This is a historiographical essay about the impact of the population exchange on the political sphere in Greece and Turkey. Notwithstanding the asymmetry in the literature produced in the two countries—excessive in Greece and limited in Turkey—there have been two seminal narratives regarding the impact of the exchange on interwar politics. In regard to Greece, the political cleavage between refugees and locals is shown as the basic factor shaping politics. In regard to Turkey, the exodus of the Christian bourgeoisie is seen as a major factor behind the authoritarianism of the interwar regime. Nevertheless, recent research on local communities that specifically explores refugee and exchangee agency in local and regional politics and studies the place of refugees and exchangees in state demographic projects goes beyond the limitations of macro perspectives, qualifying the prevailing theses on the impact of the exchange on politics in both countries.
Xylaria sridharii sp. nov.—a new species of Xylariaceae from India
Authors: SHARATHCHANDRA KODANDOOR , MAHADEVAKUMAR SHIVANNEGOWDA , NUTHAN BETTADAPURA R. , SATISH SHREEDHARAMURTHY
Published: November 2023

A new species of Xylaria associated with leaflets of coconut (Cocos nucifera) in Konaje, Mangalore, Karnataka is collected and examined. The species was characterized by having long stromata terminated with a globose head-like swollen fertile part, which showed a further extended thread-like appendage at its tip. Micro-morphological examination, cultural characterization and molecular sequence analysis of ITS-rDNA, LSU and TUB were carried out. The phylogenetic analysis based on combined ITS-rDNA and tub2 gene sequences showed that Xylaria sridharii is phylogenetically related to X. oxyacanthae, X. reevesiae and X. palmicola. However, X. sridharii is distinguished by the presence of the hirsute stromata, which shows multiple branching (2–4, but rarely recorded up to 6) at the apex, and each branch produces a fertile part, which in turn ends with a thin filiform appendage.
A new genus of Afrotropical Lasiocampini: Revaya gen. n. (Lepidoptera, Lasiocampidae, Lasiocampinae)
Authors: PROZOROV ALEXEY M. , PROZOROVA TATIANA A. , YAKOVLEV ROMAN V. , VOLKOVA JULIA S. , SALDAITIS AIDAS , SULAK HARALD , REVAY EDITA E. , MÜLLER GÜNTER C.
Published: November 2023

A new genus Revaya gen. n. with Revaya yahya sp. n. (type-species) and Revaya edita sp. n. are described. A detailed diagnosis for the new genus is provided and compared with three closely related genera: Catalebeda Aurivillius, 1902; Ptyssophlebia Berio, 1937; and Oplometa, Aurivillius 1894. Male genitalia of Oplometa cassandra (Druce, 1887) and female genitalia of Ptyssophlebia discocellularis (Strand, 1912) are illustrated for the first time. Catalebeda producta, P. discocellularis and O. cassandra are recorded for the first time in the Democratic Republic of the Congo.
Dryocoetiops krivetsae sp. n. (Coleoptera: Curculionidae: Scolytinae: Dryocoetini), the northernmost species of the genus: conflicts between molecular and morphological data in the tribe Dryocoetini
Authors: KERCHEV IVAN A. , ILINSKY YURY YU. , BYKOV ROMAN A. , MANDELSHTAM MIKHAIL YU.
Published: November 2023

A new species of bark beetle, Dryocoetiops krivetsae Kerchev, Mandelshtam, Bykov et Ilinsky, sp. n., from the southern part of Primorsky Krai (Russian Far East), is described. This is the northernmost discovery of the Oriental genus Dryocoetiops Schedl, 1957, and the first record of the genus in the Russian fauna. Phylogenetic analysis of nuclear and mitochondrial genes i) confirms an independent lineage of Dryocoetiops krivetsae and ii) indicates numerous conflicts of genera relationships in the tribe Dryocoetini.
From Tolerance for Ambiguity to Stress and Anxiety: The Mediating Role of Need for Cognitive Closure Among Chinese University Students
Authors: Zuo Tingting
Published: November 2023

Based on the Entropy Model of Uncertainty, this study examined the relationship between tolerance for ambiguity and stress and anxiety, as well as the mediating roles of overall need for cognitive closure and its dimensions in this relationship. Four hundred and eighty-eight Chinese university students were recruited online and completed the survey voluntarily and anonymously. The results of structural equation modeling revealed that tolerance for ambiguity negatively predicted students’ perceived stress and anxiety. Moreover, overall need for cognitive closure, as well as its dimensions of preference for order and decisiveness played full mediating roles in this relationship. Specifically, tolerance for ambiguity negatively predicted students’ overall need for cognitive closure, preference for order, preference for predictability, decisiveness, and closed-mindedness. Further, preference for order negatively predicted students’ perceived stress and anxiety, while overall need for cognitive closure and decisiveness positively predicted such. However, preference for predictability and closed-mindedness were not significant predictors of perceived stress and anxiety.
Structural and molecular biology of Sabiá virus
Authors: Bezerra Eduardo HS , Melo-Hanchuk Talita D , Marques Rafael Elias
Published: November 2023

Brazilian mammarenavirus, or Sabiá virus (SABV), is a New World (NW) arenavirus associated with fulminant hemorrhagic disease in humans and the sole biosafety level 4 microorganism ever isolated in Brazil. Since the isolation of SABV in the 1990s, studies on viral biology have been scarce, with no available countermeasures against SABV infection or disease. Here we provide a comprehensive review of SABV biology, including key aspects of SABV replication, and comparisons with related Old World and NW arenaviruses. SABV is most likely a rodent-borne virus, transmitted to humans, through exposure to urine and feces in peri-urban areas. Using protein structure prediction methods and alignments, we analyzed shared and unique features of SABV proteins (GPC, NP, Z, and L) that could be explored in search of therapeutic strategies, including repurposing intended application against arenaviruses. Highly conserved catalytic activities present in L protein could be targeted for broad-acting antiviral activity among arenaviruses, while protein-protein interactions, such as those between L and the matrix protein Z, have evolved in NW arenaviruses and should be specific to SABV. The nucleoprotein (NP) also shares targetable interaction interfaces with L and Z and exhibits exonuclease activity in the C-terminal domain, which may be involved in multiple aspects of SABV replication. Envelope glycoproteins GP1 and GP2 have been explored in the development of promising cross-reactive neutralizing antibodies and vaccines, some of which could be repurposed for SABV. GP1 remains a challenging target in SABV as evolutive pressures render it the most variable viral protein in terms of both sequence and structure, while antiviral strategies targeting the Z protein remain to be validated. In conclusion, the prediction and analysis of protein structures should revolutionize research on viruses such as SABV by facilitating the rational design of countermeasures while reducing dependence on sophisticated laboratory infrastructure for experimental validation.
False negative result of polymerase chain reaction in very early stages of acute retinal necrosis
Authors: Wang Haibo , Qian Zhuyun , Cui Lin , Liu Beichen , Zou Jixin , Wang Lu , Tao Yong , Zhang Lijun , Jin Lei
Published: November 2023

Background

Viral nucleic acid testing of intraocular fluid using polymerase chain reaction (PCR) is a major laboratory examination in the diagnosis of acute retinal necrosis (ARN). Importantly, false negative PCR results may occur in several special situations. We reported a case of ARN with a negative PCR result in the aqueous humour in the very early stages of disease.

Case presentation

A female patient presented to the ophthalmologist with complaints of blurred vision and redness in her left eye. Her medical history included ARN in her right eye 10 years prior. Although the result of the aqueous viral analysis by PCR in her left eye was negative the first time (one day after the appearance of ocular symptoms), ARN in her left eye was presumed based on the clinical signs. With timely antiviral and anti-inflammatory treatments, the retinal lesions diminished. The viral load of herpes simplex virus (HSV) turned positive (7.25 × 103 copies/mL) one week later, increased to 2.49 × 105 copies/mL after three weeks, and finally turned negative about five weeks after the onset of disease. The initial HSV-IgG level in the aqueous humour was 0.01 U/mL and increased to 222.64 U/mL in the final sampling.

Conclusions

The results of PCR analysis can be negative in the very early stages of ARN. Diagnosis of ARN should be made based on the clinical features, and antiviral treatments should not be delayed. Repeated PCR analysis of the aqueous humour is necessary to confirm the diagnosis and monitor the disease process.
The expression of the NPR1-dependent defense response pathway genes in Persea americana (Mill.) following infection with Phytophthora cinnamomi
Authors: Backer Robert , Naidoo Sanushka , van den Berg Noëlani
Published: November 2023

A plant’s defense against pathogens involves an extensive set of phytohormone regulated defense signaling pathways. The salicylic acid (SA)-signaling pathway is one of the most well-studied in plant defense. The bulk of SA-related defense gene expression and the subsequent establishment of systemic acquired resistance (SAR) is dependent on the nonexpressor of pathogenesis-related genes 1 (NPR1). Therefore, understanding the NPR1 pathway and all its associations has the potential to provide valuable insights into defense against pathogens. The causal agent of Phytophthora root rot (PRR), Phytophthora cinnamomi, is of particular importance to the avocado (Persea americana) industry, which encounters considerable economic losses on account of this pathogen each year. Furthermore, P. cinnamomi is a hemibiotrophic pathogen, suggesting that the SA-signaling pathway plays an essential role in the initial defense response. Therefore, the NPR1 pathway which regulates downstream SA-induced gene expression would be instrumental in defense against P. cinnamomi. Thus, we identified 92 NPR1 pathway-associated orthologs from the P. americana West Indian pure accession genome and interrogated their expression following P. cinnamomi inoculation, using RNA-sequencing data. In total, 64 and 51 NPR1 pathway-associated genes were temporally regulated in the partially resistant (Dusa®) and susceptible (R0.12) P. americana rootstocks, respectively. Furthermore, 42 NPR1 pathway-associated genes were differentially regulated when comparing Dusa® to R0.12. Although this study suggests that SAR was established successfully in both rootstocks, the evidence presented indicated that Dusa® suppressed SA-signaling more effectively following the induction of SAR. Additionally, contrary to Dusa®, data from R0.12 suggested a substantial lack of SA- and NPR1-related defense gene expression during some of the earliest time-points following P. cinnamomi inoculation. This study represents the most comprehensive investigation of the SA-induced, NPR1-dependent pathway in P. americana to date. Lastly, this work provides novel insights into the likely mechanisms governing P. cinnamomi resistance in P. americana.
Morphological characterization and interspecific variation among five species of Ziziphus genus to select superiors in Iran
Authors: Khadivi Ali
Published: November 2023

Background

Several species of the genus Ziziphus are used worldwide for their medicinal and therapeutic properties. The present study aimed to investigate the phenotypic variation of five species of the Ziziphus genus, including Z. jujuba Mill. (25 accessions), Z. mauritiana Lam. (25 accessions), Z. spina-christi L. (25 accessions), Z. nummularia L. (10 accessions), and Z. xylopyrus Willd. (10 accessions) from Markazi, Sistan-va-Baluchestan, and Khuzestan provinces, Iran.

Results

The investigated accessions have significant differences in terms of all the measured as revealed using analysis of variance (ANOVA, P < 0.01). The range of fruit weight was 0.43–1.29 g in Z. jujuba, 17.85–29.87 g in Z. mauritiana, 0.94–3.44 g in Z. spina-christi, 0.93–2.02 g in Z. nummularia, and 0.91–3.02 g in Z. xylopyrus. All the measured traits showed significant and positive correlations with each other. Multiple regression analysis (MRA) results showed that fruit length, stone width, stone weight, stone length, and fruit width have significant effects on fruit weight, and thus their fluctuations have a significant effect on increasing or decreasing fruit weight. The accessions were grouped into two main clusters using hierarchical cluster analysis. The first cluster (I) included all the accessions of Z. mauritiana, while the second cluster (II) contained the accessions of the rest species forming two sub-clusters.

Conclusion

Based on the commercial characters, accessions no. 12, 13, 17, 23, and 24 in Z. jujuba, accessions no. 3, 9, 17, 18, 20, 22, and 23 in Z. mauritiana, accessions no. 5, 6, 8, 13, 19, 22, and 24 in Z. spina-christi, accessions no. 3, 7, and 9 in Z. nummularia, and accessions no. 2, 4, 7, and 10 in Z. oxyphylla showed the highest fruit weight and thus can be suggested as superior for cultivation or use in breeding programs due to having larger fruits.
Prevalence and risk factors of oral human papillomavirus infection among 4212 healthy adults in Hebei, China
Authors: Yu Shuting , Zhu Yingying , He Huijing , Hu Yaoda , Zhu Xiaoli , Diao Wenwen , Li Shuguang , Shan Guangliang , Chen Xingming
Published: November 2023

Background

Human papillomavirus (HPV) infection is an essential cause of oropharyngeal squamous cell carcinoma that is increasing in incidence worldwide. However, little is known about the epidemiology of oral HPV infection among healthy adults in China.

Methods

A study in northern China was conducted in 2021 as baseline data of Diverse Life-Course Cohort (DLCC). Residents who aged above 20 were eligible to participate. Oral swab specimens and questionnaires were collected from 4226 participants. HPV DNA in oral exfoliated cells was tested by Nested Polymerase Chain Reaction approach and sequencing. Univariate and multivariate analyses were performed to assess the associations between exposure factors and oral HPV infection.

Results

Overall prevalence of oral HPV infection was 4.08% (95%CI, 3.69%-4.68%). The most prevalent HPV type detected was HPV-81 (1.35%; 95% CI, 1.00%–1.70%), followed by HPV-16 (0.64%; 95% CI, 0.40%–0.88%). Oral HPV infection presented a bimodal pattern with respect to age in male and female participants. Oral HPV prevalence of male participants was significantly higher than prevalence of female participants (5.0% versus 3.6%, P = 0.041). Prevalence of oral HPV was higher among current smokers (OR = 1.59; 95% CI, 1.11–2.29; P = 0.039) and current drinkers (OR = 1.60; 95% CI, 1.14–2.25; P = 0.023). Current alcohol consumption was independently associated with oral HPV infection (OR = 1.74; 95% CI, 1.22–2.50; P = 0.010).

Conclusions

Among healthy adults aged above 20 in Hebei, China, the prevalence of high-risk HPV infection was 1.92% (95%CI, 1.51%-2.34%). Oral HPV prevalence was independently associated with alcohol consumption. More tailored prevention strategies are needed to prevent oral HPV infection through smoking cessation, reduction of alcohol consumption, and HPV vaccination.
The effect of zoledronic acid on hip geometry in renal transplant recipients: a double-blind placebo-controlled randomized study
Authors: Dabbaghmanesh Alireza , Bakhshayeshkaram Marzieh , Roshanzamir Sharareh , Naseri Arzhang , Dabbaghmanesh Mohammad Mahdi , Heydari Seyed Taghi , Talehzadeh Pedram , Dabbaghmanesh Mohammad Hossein , Jahromi Shahrokh Ezzatzadegan
Published: November 2023

Background

In renal transplant patients, bisphosphonates may prevent bone loss, but little is known about their effects on bone microarchitecture and geometrical hip parameters, as the key factors of bone stability. This study aimed to analyze the effect of zoledronic acid on the mentioned parameters in kidney transplant patients.

Methods

In this double-blind, randomized trial, 33 patients were followed for six months after administering either 4mg of zoledronic acid or a placebo. Bone mineral density (BMD) measurement of the spine, hip, radius, and whole body was obtained, and trabecular bone score (TBS) was evaluated using the software. Geometric assessment at the proximal femur was performed by the HSA program.

Results

Eighteen patients in the intervention group and 15 in the control group completed the study. The mean percentages of the changes in the BMD at the lumbar spine and whole body were significantly different between the placebo and intervention groups (-0.23% vs. 4.91% and -2.03% vs. 1.23%) (P < 0.05). Zoledronic acid appeared to enhance the subperiosteal diameter, endocortical diameter, and cross-sectional moment of inertia (CSMI) at the narrow neck in comparison with placebo (P < 0.05); however, no difference in TBS was observed between both groups (P > 0.05).

Conclusions

We concluded that a single administration of zoledronic acid might ameliorate bone loss at the lumbar spine and the whole body and maintain the subperiosteal diameter, endocortical diameter, and CSMI as parameters of bone strength at the narrow neck of the proximal femur after six months in renal-transplant recipients.

Trial registration

This study was registered in IRCT (ID: IRCT20181202041821N1) on 04–05-2019.