bluuebunny/crossref_metadata_2025 huggingface dataset:
Measurement of Water Drop Sizes Generated by a Dripping Rainfall Simulator with Drippers in the Form of Hypodermic Needles
Authors: Rončević Vukašin , Živanović Nikola , Boxel John H. van , Iserloh Thomas , Antić Nevena , Ferreira Carla Sofia Santos , Spasić Marko
Published: August 2024

Dripping rainfall simulators (DRS) for soil research generate water drops with different types of drippers, but metal tubes are most commonly used, often in the form of hypodermic needles. However, scientific papers using dripping rainfall simulators are often incomplete in terms of data on hypodermic needle characteristics, as well as data on drops produced by hypodermic needles under different water pressures. This study determines which drop sizes and dripping speeds are generated by various hypodermic needles at different water pressures. For the purpose of this study, a dripping rainfall simulator was designed and constructed for laboratory use. Water drops were generated with 11 different needles, ranging in size from 16 G to 32 G (tube gauge number), at different water pressures. Measured water drop sizes ranged from 1.42 to 3.69 mm at a dripping speed between 10 and 360 drops per minute and water head from 14 to over 1970 mm. Measured drop sizes, supplemented with data from previous studies, provided information on the relation between drop sizes and the size of the hypodermic needles. Van Boxel’s numerical model provided estimations of the fall velocity for different drop diameters and their kinetic energy for falling heights up to 11.5 m. The results of this research can be used to design dripping rainfall simulators for soil research.
NPS6D200—A Long Range Nanopositioning Stage with 6D Closed Loop Control
Authors: Hesse Steffen , Huaman Alex , Katzschmann Michael , Leistritz Bianca , Herzog Ludwig
Published: August 2024

This work presents the new development of a nanopositioning machine for a large operating range. The machine, called NPS6D200, provides Ø200 mm planar and 25 mm vertical travel range and applies a 6D closed loop control with all drive forces applied directly to the same moving part. The stage architecture evolves from an integrated planar direct drive which is extended by high precision vertical positioning capability. The setup of the machine and the characteristics of the different subsystems are presented together with investigations into the positioning performance that is achieved with the NPS6D200. In constant setpoint operation as well as in synchronized multiaxial motion tasks over three orders of magnitude, the system shows servo errors only in the low nanometer range and proves suitable as positioning platform for nanoscience applications.
Applicability of Magnetic Susceptibility Measurements on Cave Sediments in Karst Areas: Insight from Dinaric Karst (Velebit Mt., Croatia)
Authors: Barudžija Uroš , Kamenski Ana , Paar Dalibor , Malvić Tomislav
Published: August 2024

Magnetism in Croatian caves has been significantly underexplored, despite the well-documented preservation of cave sediments. This study investigates whether magnetic susceptibility can be effectively recorded in Croatian caves and, if so, whether there are differences between in situ and laboratory measurements, as well as the extent of these differences. The focus is on two prominent sites within the Velebit Mountain range of the Dinaric Karst: the deep Slovačka Cave and the extensive Crnopac Cave System (ex. Kita Gaćešina Cave). Magnetic susceptibility was measured in soft sediments using the Kappameter KM-7 for in situ assessments and the Bartington MS2B System for laboratory analyses. The comparison of the two devices aimed to evaluate the feasibility of detecting magnetic susceptibility variations within carbonate sediments. Sampling locations were selected based on preliminary assessments of magnetic susceptibility variations and accessibility for sampling. Characteristic samples from each profile underwent X-ray powder diffraction analysis. Results revealed significant variations in magnetic susceptibility across each profile under both in situ and laboratory conditions. In situ measurements ranged from −45 × 10−5 SI to 120 × 10−5 SI, while laboratory measurements ranged from 4.00 × 10−5 SI to 99.40 × 10−5 SI. The portable Kappameter KM-7 provided satisfactory results for preliminary investigations, while the Bartington MS2B System demonstrated high precision in analyzing these variations in detail. These findings support the potential for further investigations into magnetic susceptibility in karst cave environments, with an emphasis on understanding depositional history and the timeline of climate and tectonic changes.
E-GTN: Advanced Terrain Sensing Framework for Enhancing Intelligent Decision Making of Excavators
Authors: Zhao Qianyou , Gao Le , Wu Duidi , Meng Xinyao , Qi Jin , Hu Jie
Published: August 2024

The shift towards autonomous excavators in construction and mining is a significant leap towards enhancing operational efficiency and ensuring worker safety. However, it presents challenges, such as the need for sophisticated decision making and environmental perception due to complex terrains and diverse conditions. Our study introduces the E-GTN framework, a novel approach tailored for autonomous excavation that leverages advanced multisensor fusion and a custom-designed convolutional neural network to address these challenges. Results demonstrate that GridNet effectively processes grid data, enabling the reinforcement learning algorithm to make informed decisions, thereby ensuring efficient and intelligent autonomous excavator performance. The study concludes that the E-GTN framework offers a robust solution for the challenges in unmanned excavator operations, providing a valuable platform for future advancements in the field.
Advancements and Challenges in Perovskite-Based Photo-Induced Rechargeable Batteries and Supercapacitors: A Comparative Review
Authors: M. R. Anil Kumar , Nekahi Atiyeh , Bouguern Mohamed Djihad , Ma Dongling , Zaghib Karim
Published: August 2024

Perovskite-based photo-batteries (PBs) have been developed as a promising combination of photovoltaic and electrochemical technology due to their cost-effective design and significant increase in solar-to-electric power conversion efficiency. The use of complex metal oxides of the perovskite-type in batteries and photovoltaic cells has attracted considerable attention. Because of its variable bandgap, non-rigid structure, high light absorption capacity, long charge carrier diffusion length, and high charge mobility, this material has shown promise in energy storage devices, especially Li-ion batteries (LIBs) and PBs. This review paper focuses on recent progress and comparative analysis of PBs using perovskite-based materials. The practical application of these batteries as dependable power sources faces significant technical and financial challenges because solar radiation is alternating. In order to address this, research is being performed on PBs with the integration of perovskite solar cells (PSCs) as a way to balance energy availability and demand, cut down on energy waste, and stabilize power output for wearable and portable electronics as well as energy storage applications.
Response Characteristics and Community Assembly Mechanisms of nirS-Type Denitrifiers in the Alpine Wetland under Simulated Precipitation Conditions
Authors: Zhang Ni , Chen Kelong , Chen Ji , Ji Wei , Yang Ziwei , Chen Zhirong
Published: August 2024

The nitrogen cycling process in alpine wetlands is profoundly affected by precipitation changes, yet the dynamic response mechanism of denitrifiers to long-term precipitation shifts in the alpine wetland of the Qinghai-Tibet Plateau remains enigmatic. Utilizing high-throughput sequencing analysis of nirS-type functional genes, this study delved into the dynamic response mechanism of nirS-type denitrifiers to precipitation changes in the alpine wetland of Qinghai Lake. The findings revealed that nirS-type denitrifiers in the alpine wetland of Qinghai Lake were primarily Proteobacteria, and Alpha diversity exhibited a negative correlation with the precipitation gradient, with deterministic processes predominating in the community assembly of denitrifying microbes. A 50% increase in rainfall shifted the community assembly process of denitrifiers from deterministic to stochastic. Dominant microflora at the genus level responded significantly to precipitation changes, with aerobic bacteria comprising the majority of differentially abundant taxa (55.56%). As precipitation increased, the complexity of the microbial interaction network decreased, and a 25% reduction in precipitation notably elevated the relative abundance of three key functional groups: chemoheterotrophic, aerobic chemoheterotrophic, and nitrogen fixation. Precipitation notably emerged as the primary regulator of nirS-type denitrifiers in the alpine wetland of Qinghai Lake, accounting for 51% of the variation in community composition. In summary, this study offers a fresh perspective for investigating the ecological processes of nitrogen cycling in alpine ecosystems by examining the diversity and community composition of nirS-type denitrifiers in response to precipitation changes.
Hypoxia-Induced Adaptations of Embryonic Fibroblasts: Implications for Developmental Processes
Authors: Li Zeyu , Han Delong , Li Zhenchi , Luo Lingjie
Published: August 2024

Animal embryonic development occurs under hypoxia, which can promote various developmental processes. Embryonic fibroblasts, which can differentiate into bone and cartilage and secrete various members of the collagen protein family, play essential roles in the formation of embryonic connective tissues and basement membranes. However, the adaptations of embryonic fibroblasts under hypoxia remain poorly understood. In this study, we investigated the effects of hypoxia on mouse embryonic fibroblasts (MEFs). We found that hypoxia can induce migration, promote metabolic reprogramming, induce the production of ROS and apoptosis, and trigger the activation of multiple signaling pathways of MEFs. Additionally, we identified several hypoxia-inducible genes, including Proser2, Bean1, Dpf1, Rnf128, and Fam71f1, which are regulated by HIF1α. Furthermore, we demonstrated that CoCl2 partially mimics the effects of low oxygen on MEFs. However, we found that the mechanisms underlying the production of ROS and apoptosis differ between hypoxia and CoCl2 treatment. These findings provide insights into the complex interplay between hypoxia, fibroblasts, and embryonic developmental processes.
Impact of BMI and Cardiorespiratory Fitness on Oxidative Stress in Plasma and Circulating Exosomes Following Acute Exercise
Authors: Slusher Aaron L. , Visavadiya Nishant P. , Fico Brandon G. , Estébanez Brisamar , Acevedo Edmund O. , Huang Chun-Jung
Published: August 2024

The impact of cardiorespiratory fitness (VO2max) and obesity on indices of oxidative stress in plasma and circulating exosome-like extracellular vesicles (ELVs) were examined following acute exercise. Indices of oxidative stress in plasma and isolated plasma ELVs were examined in aerobically trained (NW-Tr; n = 15) and untrained (NW-UTr; n = 18) normal-weight individuals and aerobically untrained individuals with obesity (Ob-Utr; n = 10) prior to and immediately following acute maximal treadmill running. Following exercise, ELV flotillin-1 expression (p = 0.008) and plasma total antioxidant capacity (TAC; p = 0.010) increased more in NW-UTr compared to NW-Tr and Ob-UTr participants, whereas plasma protein carbonyls (PC) decreased more in Ob-UTr compared to NW-Tr and NW-UTr groups. ELV glutathione (GSH) concentrations decreased more in NW-Tr compared to NW-UTr and Ob-UTr participants (p = 0.009), whereas lipid peroxidase (LPO) concentrations increased more in Ob-UTr compared to NW-Tr and NW-UTr participants (p = 0.003). Body mass index (BMI) was associated negatively with plasma TAC and PC (p < 0.05) and positively with ELV LPO concentration responses (p = 0.009). Finally, plasma-to-total (plasma + ELV) GSH ratios decreased in Ob-UTr compared to NW-Tr and NW-UTr participants (p = 0.006), PC ratios increased in NW-Tr and NW-UTr compared to Ob-UTr subjects (p = 0.008), and reactive oxygen/nitrogen species ratios increased in NW-UTr and decreased in Ob-UTr participants (p < 0.001). BMI, independently of VO2max, differentially regulates indices of oxidative stress within plasma and circulating ELVs prior to and immediately following acute maximal treadmill exercise.
Pathophysiology in Brain Arteriovenous Malformations: Focus on Endothelial Dysfunctions and Endothelial-to-Mesenchymal Transition
Authors: Jeong Jae Yeong , Bafor Adrian E. , Freeman Bridger H. , Chen Peng R. , Park Eun S. , Kim Eunhee
Published: August 2024

Brain arteriovenous malformations (bAVMs) substantially increase the risk for intracerebral hemorrhage (ICH), which is associated with significant morbidity and mortality. However, the treatment options for bAVMs are severely limited, primarily relying on invasive methods that carry their own risks for intraoperative hemorrhage or even death. Currently, there are no pharmaceutical agents shown to treat this condition, primarily due to a poor understanding of bAVM pathophysiology. For the last decade, bAVM research has made significant advances, including the identification of novel genetic mutations and relevant signaling in bAVM development. However, bAVM pathophysiology is still largely unclear. Further investigation is required to understand the detailed cellular and molecular mechanisms involved, which will enable the development of safer and more effective treatment options. Endothelial cells (ECs), the cells that line the vascular lumen, are integral to the pathogenesis of bAVMs. Understanding the fundamental role of ECs in pathological conditions is crucial to unraveling bAVM pathophysiology. This review focuses on the current knowledge of bAVM-relevant signaling pathways and dysfunctions in ECs, particularly the endothelial-to-mesenchymal transition (EndMT).
Genetic and Clinical Factors Influencing Congenital Anomalies of the Kidney and Urinary Tract in Children: Insights from Prenatal and Postnatal Assessments
Authors: Önal Hülya Gözde , Nalçacıoğlu Hülya , Karalı Demet Tekcan , Önal Mesut , Yağız Beytullah , Bilgici Meltem Necibe Ceyhan
Published: August 2024

Congenital anomalies of the kidney and urinary tract (CAKUT) significantly contribute to pediatric morbidity, often necessitating ureterorenal surgery. This study explored the relationship between genetic mutations, renal surgery requirements, and prenatal, postnatal, and parental risk factors in children with CAKUT. A retrospective analysis of 651 children diagnosed with CAKUT included patient demographics, parental risk factors, ultrasound findings, genetic mutations, and surgical incidence. Antenatal ultrasounds showed normal findings in 32.1%, hydronephrosis in 46.9%, and other abnormalities in 21% of cases. Surgical intervention correlated with higher hydronephrosis reduction than non-intervention. Genetic mutations were identified in 5.4% of cases, with 24.6% requiring surgery. Low neonatal birth weight (odds ratio [OR] = 0.98, p < 0.001), advanced maternal age (OR = 1.06, p < 0.001), and postnatal abnormal ultrasound findings (OR = 2.62, p < 0.001) were associated with increased genetic mutation risks. Antenatal hydronephrosis (OR = 3.85, p < 0.001) and postnatal urinary tract infections (OR = 4.85, p < 0.001) increased the likelihood of surgical intervention. Neonatal birth weight, maternal age, and postnatal ultrasound findings were identified as independent risk factors for genetic mutations, while no significant link was found between these genetic factors and the need for surgery. Surgical needs were associated with urinary tract infections and antenatal hydronephrosis, indicating that timely surgical intervention may benefit these patients.
A Fluorescent Lateral Flow Immunoassay for the Detection of Skeletal Muscle Troponin I in Serum for Muscle Injury Monitoring at the Point of Care
Authors: Tang Deding , Wu Shuang , Kong Mengqi , Liu Zhaonan , Li Zonghao , Han Ying , Gong Yan , Hu Jie
Published: August 2024

Exercise-induced muscle injury is one of the most common types of sports injuries. Skeletal muscle troponin I (skTnI) serves as an ideal biomarker in assessing such injuries, facilitating timely detection and evaluation. In this study, we develop a fluorescent sandwich lateral flow immunoassay (LFIA) combined with a desktop analyzer for rapid detection of skTnI. Through optimizing the reaction system, the assay achieves a satisfying detection performance, reaching a limit of detection (LOD) of 0.5 ng/mL with a turnaround time of 15 min. The proposed detection platform offers portability, ease of use, and high sensitivity, which facilitates the monitoring of exercise-induced muscle injuries at the point of care. This feature is particularly advantageous for end users, enabling timely detection of sports-related injuries and ultimately enhancing prognosis and sports life.
Electrical Conductivity Measurement in Human Liver Tissue: Assessment on Normal vs. Tumor Tissue and under In Vivo vs. Ex Vivo Conditions
Authors: Sarreshtehdari Amirhossein , García-Sánchez Tomás , Sánchez-Velázquez Patricia , Ielpo Benedetto , Berjano Enrique , Villamonte María , Moll Xavier , Burdio Fernando
Published: August 2024

Background: This study evaluated electrical conductivity in human liver tissue in the 3–1000 kHz frequency range to compare normal versus tumor tissues under in vivo versus ex vivo conditions. Methods: Previous informed consent was obtained from twenty patients undergoing liver resection in whom liver electrical conductivity was measured during surgery and after resection. Result: We found higher electrical conductivity values in tumor tissues than in normal tissue in both in vivo (0.41 ± 0.10 vs. 0.13 ± 0.06 S/m) and ex vivo (0.27 ± 0.09 vs. 0.12 ± 0.07 S/m) conditions (at 3 kHz). The electric properties also showed a promising potential for distinguishing between different tissue types including metastasis, cholangiocarcinoma (CCA), hepatocellular carcinoma (HCC), hepatic cirrhosis, and normal liver (both in vivo and ex vivo). At 3 kHz, in vivo electrical conductivity for cholangiocarcinoma, HCC, and metastasis were 0.35, 0.42 ± 0.13, and 0.41 ± 0.08 S/m, respectively, which differed significantly from each other (p < 0.05). Conclusions: These findings could potentially improve liver disease diagnostics through electrical conductivity measurements and treatment techniques involving electric fields. Future research should focus on expanding the sample size to refine the categorization and comparison processes across diverse human liver tissue types.
Enhancing Job Performance: The Critical Roles of Well-Being, Satisfaction, and Trust in Supervisor
Authors: Yang Yiting , Obrenovic Bojan , Kamotho Daniel W. , Godinic Danijela , Ostic Dragana
Published: August 2024

This empirical study investigated the associations between psychological well-being, job satisfaction, trust in supervisor, and job performance. Data were collected from 277 company employees in Bahrain through online questionnaires and analyzed using structural equation modeling (SEM). The purpose of the study is to examine the relationships between psychological well-being, job satisfaction, trust in supervisor, and job performance through the use of a theoretical framework that synthesizes prominent models in the field of organizational psychology. The research findings indicate a positive influence of psychological well-being on both job satisfaction and job performance. Notably, job satisfaction plays a mediating role in the relationship between psychological well-being and job performance. The study contributes to the existing body of knowledge by offering an integrated approach to examining the intricate connections between psychological well-being, job satisfaction, trust in supervisor, and job performance, which all are crucial for creating a sustainable workplace environment and outcomes. Practical implications highlight the need for organizations to prioritize employee psychological well-being through initiatives such as wellness programs and supportive work environments, as these initiatives directly influence job satisfaction and performance. Job satisfaction acts as a significant mediator, emphasizing the importance of fair compensation, recognition, and professional development in enhancing job satisfaction.
Analysis of Factors Contributing to State Body Appreciation during Exercise
Authors: Baceviciene Migle , Bliujute Kristina , Jankauskiene Rasa
Published: August 2024

This cross-sectional study aimed to analyse the factors contributing to state body appreciation (SBA) during exercise. After providing their informed consent, 200 study participants (mean age 30.0 ± 9.4 years, 72.0% were men) filled in an online questionnaire immediately after the completion of resistance training (n = 125), cardiovascular exercise (n = 55), or functional/interval group exercise (n = 20) sessions. The study measures included socio-demographic variables, body mass index (BMI), the duration of involvement in sports, SBA, state body surveillance (SBS), state mindfulness in physical activity, state intrinsic exercise regulation, and perceived pleasantness during exercise. The results showed that exercisers involved in sports for >2 years and those whose body weight was within a healthy range (<25.0 kg/m2) demonstrated higher SBA and mindfulness during exercise, lower SBS, more intrinsic exercise regulation, and higher satisfaction during physical activity compared to exercisers with less exercise experience (≤2 years) and a body weight higher than a healthy range (≥25.0 kg/m2). The negative effects of being overweight or obese were more pronounced in individuals with ≤2 years of sports participation, except regarding body surveillance and monitoring the mind. The factors significantly contributing to SBA during the exercise sessions were a longer duration of involvement in sports, a lower BMI and SBS, and greater mindful body acceptance and exercise pleasantness. Decreasing SBS and enhancing mindful body acceptance, pleasantness, and intrinsic motivation during exercise might significantly contribute to SBA in physical activity. These results can inform physical-activity-based programmes aiming to promote a positive body image. Also, these results show that it is important to increase education and develop competencies for fitness coaches to create inclusive and positive-body-image-promoting sports environments.
Preparation and Performance Testing of Steel Slag Concrete from Steel Solid Waste
Authors: Yuan Bao , Zhao Dongnan , Lei Jialiu , Song Shengqiang
Published: August 2024

In this paper, steel slag powder was used to replace part of the cement in road concrete, and group tests were carried out on coarse aggregates with different water–cement (W/C) ratios, different steel slag parameters, and different particle sizes. A sample of 100 mm × 100 mm × 100 mm was prepared and cured for 3 days, 7 days, and 28 days. In addition, the fluidity and compressive properties of the samples were also tested. The outcomes of this study revealed that, at a constant W/C ratio, increasing the proportion of steel slag improved the concrete’s fluidity but reduced its compressive strength; the 3-day (3 d) compressive strength of 40% steel slag was lower because the early activity of steel slag was lower than that of cement; steel slag also decreased the early-hydration rate of concrete. Comparisons across different W/C ratios demonstrated that steel slag made a more significant contribution to lower W/C ratios than higher ones. The water requirement for steel slag was relatively moderate, and the compressive strength of steel slag concrete with a high W/C ratio notably improved in the later stages. Based on the experimental conditions, the optimal content of steel slag was found to be 35%. Reusing steel slag as a replacement for sand in coarse aggregate can effectively lower costs and offer an innovative approach to steel slag treatment.
A Comparative Study of the Fire Properties of Chinese Traditional Timber Structural Components under Different Surface Treatments
Authors: Li Yupeng , Yeo Sokyee , Dai Shibing
Published: August 2024

Fire is generally recognized as a major threat to the protection of historic timber architecture. Thus, there is an urgent need to study the fire properties of historic timber structures so as to better protect them in the future. Two types of commonly used wood species (pine and poplar) were selected as test specimens and three types of surface treatments (Chinese traditional coating, modern flame retardant, and a combination of the two methods) were applied. The specimens were subjected to a semi-full-scale fire test. The charring rate and the surface heating curve were calculated during the fire test to assess the flammability of various woods under different treatments. Results showed that the fire properties of traditional-coated wood were better than the modern type, but large amounts of smoke were released during combustion. The combination of traditional and modern methods did not significantly improve the overall fire properties and was even worse than the traditional treatment alone. At the same time, the fire properties of the modern method are highly correlated with the type of wood species used. The above results can provide informative advice on the selection of suitable surface treatments for the subsequent restoration of ancient buildings.
Research on Urban Community Street Environment Evaluation and Optimization Strategy under the Concept of a Healthy City: A Case Study of the Dingwangtai Area of Changsha City
Authors: Zhang Yichi , Tang Hui , Huo Kecheng , Tang Jiangfan
Published: August 2024

The World Health Organization (WHO) articulated a vision in 1986, hoping that countries around the world would actively promote the construction and development of healthy cities. In the context of China, a paradigm shift in urban development has been triggered by the deep implementation of the Healthy China Strategy and the gradual completion of the comprehensive national spatial planning. This shift emphasizes a micro-level focus, advocating a human-centered approach to urban space exploration. In this paper, the Dingwangtai Area of Changsha City is selected as a case study. A street space health evaluation index system called “5D+” is constructed from six dimensions, namely, human perception, degree of mixing, density, distance to transit, destination accessibility, and devise. This research adopts the community as the fundamental unit of analysis, employing the Entropy-weighting TOPSIS method for the computation of indicators. The results of this study show that the Dingwangtai Area exhibits relative deficiencies in the dimensions of density, devise, and destination accessibility. Specifically, at the community level, the Ma Wang Street Community and the Fanhou Street Community have scored particularly low. In response, this paper proposes targeted measures and detailed recommendations aimed at optimizing the design of the street, enhancing the human experience, enriching functional attributes, and refining the street network. The ultimate goal is to propose a Healthy Streets Evaluation Index System based on the concept of a healthy city and to explore its relationship with healthy streets so as to provide valuable insights for the development of healthy city streets.
Evaluation of TBM Cutter Wear in Granite and Developing a Cutter Life Prediction Model for Face Cutters Based on Field Data: A Case Study
Authors: Liu Jianping , He Tiankui , Peng Xingxin , Pan Yucong
Published: August 2024

Disc cutter wear has emerged as a critical concern impacting the efficiency and cost budgets of TBMs (tunnel boring machines). Through statistical analysis of field data on cutter wear in a TBM tunnel, this study explores the wear rules of different types of disc cutters in granite. Grey sensitivity analysis is employed to investigate the sensitivity between the cutter ring wear rate of face cutters and two types of cutter wear influence parameters. Subsequently, reasonable parameters are selected to develop a new cutter life prediction model for face cutters. The results show that, with increases in the installation radius, the accumulated wear extent shows a linearly increasing trend for both the center and the face cutter, while it first increases and then decreases for gauge cutters, and the accumulated replacement number shows a linear growth trend for face cutters. The accumulated wear extent of the average single cutter position of gauge cutters is about 3 times that of face cutters and 7 times that of center cutters; the number of replaced cutter rings of the average single cutter position for gauge cutters is about 3–4 times that for center cutters and face cutters; and the average utilization rate of gauge cutters is the highest (80.97%). The cutter ring wear rate of face cutters is the most sensitive to three intact rock parameters (uniaxial compressive strength (UCS), Cerchar abrasion index (CAI), and equivalent quartz content (EQC)) and two TBM tunnelling parameters (cutterhead thrust (F) and cutterhead rotational speed (RPM)). Finally, a new cutter life prediction model (R2 = 0.964) for face cutters is developed based on F, UCS, and RPM. The research results can provide a certain theoretical basis for cutter wear and cutter life prediction for the face cutters of TBM projects in similar geological conditions and TBM specifications.
SKF-96365 Expels Tyrosine Kinase Inhibitor-Treated CML Stem and Progenitor Cells from the HS27A Stromal Cell Niche in a RhoA-Dependent Mechanism
Authors: Dubourg Audrey , Harnois Thomas , Cousin Laetitia , Constantin Bruno , Bourmeyster Nicolas
Published: August 2024

Background: A major issue in Chronic Myeloid Leukemia (CML) is the persistence of quiescent leukemia stem cells (LSCs) in the hematopoietic niche under tyrosine kinase inhibitor (TKI) treatment. Results: Here, using CFSE sorting, we show that low-proliferating CD34+ cells from CML patients in 3D co-culture hide under HS27A stromal cells during TKI treatment—a behavior less observed in untreated cells. Under the same conditions, Ba/F3p210 cells lose their spontaneous motility. In CML CD34+ and Ba/F3p210 cells, while Rac1 is completely inhibited by TKI, RhoA remains activated but is unable to signal to ROCK. Co-incubation of Ba/F3p210 cells with TKI, SKF-96365 (a calcium channel inhibitor), and EGF restores myosin II activation and amoeboid motility to levels comparable to untreated cells, sustaining the activation of ROCK. In CFSE+ CD34+ cells containing quiescent leukemic stem cells, co-incubation of TKI with SKF-96365 induced the expulsion of these cells from the HS27A niche. Conclusions: This study underscores the role of RhoA in LSC behavior under TKI treatment and suggests that SKF-96365 could remobilize quiescent CML LSCs through reactivation of the RhoA/ROCK pathway.
Exploring Predictive and Prognostic Biomarkers in Colorectal Cancer: A Comprehensive Review
Authors: Ashouri Karam , Wong Alexandra , Mittal Pooja , Torres-Gonzalez Lesly , Lo Jae Ho , Soni Shivani , Algaze Sandra , Khoukaz Taline , Zhang Wu , Yang Yan , Millstein Joshua , Lenz Heinz-Josef , Battaglin Francesca
Published: August 2024

Colorectal cancer (CRC) remains the second leading cause of cancer-related mortality worldwide. While immune checkpoint inhibitors have significantly improved patient outcomes, their effectiveness is mostly limited to tumors with microsatellite instability (MSI-H/dMMR) or an increased tumor mutational burden, which comprise 10% of cases. Advancing personalized medicine in CRC hinges on identifying predictive biomarkers to guide treatment decisions. This comprehensive review examines established tissue markers such as KRAS and HER2, highlighting their roles in resistance to anti-EGFR agents and discussing advances in targeted therapies for these markers. Additionally, this review summarizes encouraging data on promising therapeutic targets and highlights the clinical utility of liquid biopsies. By synthesizing current evidence and identifying knowledge gaps, this review provides clinicians and researchers with a contemporary understanding of the biomarker landscape in CRC. Finally, the review examines future directions and challenges in translating promising biomarkers into clinical practice, with the goal of enhancing personalized medicine approaches for colorectal cancer patients.
Targeting IL-8 and Its Receptors in Prostate Cancer: Inflammation, Stress Response, and Treatment Resistance
Authors: McClelland Shauna , Maxwell Pamela J. , Branco Cristina , Barry Simon T. , Eberlein Cath , LaBonte Melissa J.
Published: August 2024

This review delves into the intricate roles of interleukin-8 (IL-8) and its receptors, CXCR1 and CXCR2, in prostate cancer (PCa), particularly in castration-resistant (CRPC) and metastatic CRPC (mCRPC). This review emphasizes the crucial role of the tumour microenvironment (TME) and inflammatory cytokines in promoting tumour progression and response to tumour cell targeting agents. IL-8, acting through C-X-C chemokine receptor type 1 (CXCR1) and type 2 (CXCR2), modulates multiple signalling pathways, enhancing the angiogenesis, proliferation, and migration of cancer cells. This review highlights the shift in PCa research focus from solely tumour cells to the non-cancer-cell components, including vascular endothelial cells, the extracellular matrix, immune cells, and the dynamic interactions within the TME. The immunosuppressive nature of the PCa TME significantly influences tumour progression and resistance to emerging therapies. Current treatment modalities, including androgen deprivation therapy and chemotherapeutics, encounter persistent resistance and are complicated by prostate cancer’s notably “immune-cold” nature, which limits immune system response to the tumour. These challenges underscore the critical need for novel approaches that both overcome resistance and enhance immune engagement within the TME. The therapeutic potential of inhibiting IL-8 signalling is explored, with studies showing enhanced sensitivity of PCa cells to treatments, including radiation and androgen receptor inhibitors. Clinical trials, such as the ACE trial, demonstrate the efficacy of combining CXCR2 inhibitors with existing treatments, offering significant benefits, especially for patients with resistant PCa. This review also addresses the challenges in targeting cytokines and chemokines, noting the complexity of the TME and the need for precision in therapeutic targeting to avoid side effects and optimize outcomes.
Interaction of Receptor-Binding Domain of the SARS-CoV-2 Omicron Variant with hACE2 and Actin
Authors: Fujimoto Ai , Kawai Haruki , Kawamura Rintaro , Kitamura Akira
Published: August 2024

The omicron variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was identified in 2021 as a variant with heavy amino acid mutations in the spike protein, which is targeted by most vaccines, compared to previous variants. Amino acid substitutions in the spike proteins may alter their affinity for host viral receptors and the host interactome. Here, we found that the receptor-binding domain (RBD) of the omicron variant of SARS-CoV-2 exhibited an increased affinity for human angiotensin-converting enzyme 2, a viral cell receptor, compared to the prototype RBD. Moreover, we identified β- and γ-actin as omicron-specific binding partners of RBD. Protein complex predictions revealed that many omicron-specific amino acid substitutions affected the affinity between RBD of the omicron variant and actin. Our findings indicate that proteins localized to different cellular compartments exhibit strong binding to the omicron RBD.
Blocking Thromboxane-Prostanoid Receptor Signaling Attenuates Lipopolysaccharide- and Stearic Acid-Induced Inflammatory Response in Human PBMCs
Authors: Rajamanickam Vinothkumar , Desouza Cyrus V. , Castillo Romilia T. , Saraswathi Viswanathan
Published: August 2024

Inflammation is implicated in the etiology of obesity-related diseases. Thromboxane-prostanoid receptor (TPR) is known to play a role in mediating an inflammatory response in a variety of cells. Gut-derived lipopolysaccharide (LPS), a TLR4 agonist, is elevated in obesity. Moreover, free fatty acids (FFAs) are important mediators of obesity-related inflammation. However, the role and mechanisms by which TPR regulates the inflammatory response in human immune cells remain unclear. We sought to determine the link between TPR and obesity and the role/mechanisms by which TPR alters LPS- or stearic acid (SA)-induced inflammatory responses in PBMCs. Cells were pre-treated with agents blocking TPR signaling, followed by treatment with LPS or stearic acid (SA). Our findings showed that TPR mRNA levels are higher in PBMCs from individuals with obesity. Blockade of TPR as well as ROCK, which acts downstream of TPR, attenuated LPS- and/or SA-induced pro-inflammatory responses. On the other hand, TPR activation using its agonist enhanced the pro-inflammatory effects of LPS and/or SA. Of note, the TPR agonist by itself elicits an inflammatory response, which was attenuated by blocking TPR or ROCK. Our data suggest that TPR plays a key role in promoting an inflammatory response in human PBMCs, and this effect is mediated via TLR4 and/or ROCK signaling.
Skin Telocytes Could Fundament the Cellular Mechanisms of Wound Healing in Platelet-Rich Plasma Administration
Authors: Manole Catalin G. , Voiculescu Vlad M. , Soare Cristina , Ceafalan Laura Cristina , Gherghiceanu Mihaela , Hinescu Mihail E.
Published: August 2024

For more than 40 years, autologous platelet concentrates have been used in clinical medicine. Since the first formula used, namely platelet-rich plasma (PRP), other platelet concentrates have been experimented with, including platelet-rich fibrin and concentrated growth factor. Platelet concentrates have three standard characteristics: they act as scaffolds, they serve as a source of growth factors and cytokines, and they contain live cells. PRP has become extensively used in regenerative medicine for the successful treatment of a variety of clinical (non-)dermatological conditions like alopecies, acne scars, skin burns, skin ulcers, muscle, cartilage, and bone repair, and as an adjuvant in post-surgery wound healing, with obvious benefits in terms of functionality and aesthetic recovery of affected tissues/organs. These indications were well documented, and a large amount of evidence has already been published supporting the efficacy of this method. The primordial principle behind minimally invasive PRP treatments is the usage of the patient’s own platelets. The benefits of the autologous transplantation of thrombocytes are significant, representing a fast and economic method that requires only basic equipment and training, and it is biocompatible, thus being a low risk for the patient (infection and immunological reactions can be virtually disregarded). Usually, the structural benefits of applying PRP are attributed to fibroblasts only, as they are considered the most numerous cell population within the interstitium. However, this apparent simplistic explanation is still eluding those different types of interstitial cells (distinct from fibroblasts) that are residing within stromal tissue, e.g., telocytes (TCs). Moreover, dermal TCs have an already documented potential in angiogenesis (extra-cutaneous, but also within skin), and their implication in skin recovery in a few dermatological conditions was attested and described ultrastructurally and immunophenotypically. Interestingly, PRP biochemically consists of a series of growth factors, cytokines, and other molecules, to which TCs have also proven to have a positive expression. Thus, it is attractive to hypothesize and to document any tissular collaboration between cutaneous administered PRP and local dermal TCs in skin recovery/repair/regeneration. Therefore, TCs could be perceived as the missing link necessary to provide a solid explanation of the good results achieved by administering PRP in skin-repairing processes.
Analysis of the Effector Functions of Vδ2 γδ T Cells and NK Cells against Cholangiocarcinoma Cells
Authors: Kulma Inthuon , Na-Bangchang Kesara , Carvallo Herrera Andrea , Ndubuisi Ifeanyi Theodora , Iwasaki Masashi , Tomono Hiromi , Morita Craig T. , Okamura Haruki , Mukae Hiroshi , Tanaka Yoshimasa
Published: August 2024

Cholangiocarcinoma (CCA) is a rare disease characterized by malignant cells derived from the epithelial cells of the biliary duct system. Despite extensive treatments, the prognosis for CCA remains poor, emphasizing the critical need for the development of novel treatments. Considerable attention has been directed towards innate immune effector cells, which can recognize tumor cells independently of the major histocompatibility complex, laying the foundation for the development of off-the-shelf drugs. In this study, we cultured innate immune cells obtained from the peripheral blood of healthy adults and conducted a comparative analysis of the effector functions against CCA cell lines by Vδ2 γδ T cells and NK cells. This analysis was performed using standard short- and long-term cytotoxicity assays, as well as ELISA for IFN-γ. Vδ2 γδ T cells demonstrated cytotoxicity and IFN-γ production in response to CCA cells in a TCR-dependent manner, particularly in the presence of tetrakis-pivaloyloxymethyl 2-(thiazole-2-ylamino)ethylidene-1,1-bisphosphonate, a bisphosphonate prodrug. In contrast, direct killing and antibody-dependent cellular cytotoxicity were relatively slow and weak. Conversely, NK cells displayed potent, direct cytotoxicity against CCA cells. In summary, both Vδ2 γδ T cells and NK cells show promise as innate immune effector cells for adoptive transfer therapy in the context of CCA.
The Impacts of Narcissistic Leadership on Achieving the United Nations’ Sustainable Development Goals—A Scoping Review
Authors: Khorram-Manesh Amir , Goniewicz Krzysztof , Burkle Frederick M.
Published: August 2024

The United Nations’ Sustainable Development Goals (UN’s SDG) are a set of actionable frameworks, which can be achieved through collaboration among nations, emphasizing the significant role of leadership. Recent geopolitical trends have spotlighted leaders with pronounced sociopathic narcissistic characteristics, opposing any collaborative approach to achieving the UN’s SDGs. This study used a mix method (a scoping review and action research), to critically investigate the impact of leadership, narcissistic leadership in particular, on achieving the UN’s SDGs. The results showed that publications discussing the impacts of narcissism on achieving interconnected SDGs are scarce. The systemic negative factors enabling narcissistic leaders to rise above and keep power, and potential strategies to inhibit such leadership styles were critically discussed. The loss of the Sustainable Development Goals remains the best indicator of the global negative impact of narcissistic leadership and its influence on global health systems. It is not country-specific and is the only legitimate global measure available. Preventing the rise of such leadership, particularly in low- and middle-income countries may avert wars, conflicts, and their deadly outcomes from national and global perspectives. Novel global policies are urgently needed to safeguard global peace, health, and security.
An AI-Enhanced Systematic Review of Climate Adaptation Costs: Approaches and Advancements, 2010–2021
Authors: Boero Riccardo
Published: August 2024

This study addresses the critical global challenge of climate adaptation by assessing the inadequacies in current methodologies for estimating adaptation costs. Broad assessments reveal a significant investment shortfall in adaptation strategies, highlighting the necessity for precise cost analysis to guide effective policy-making. By employing the PRISMA 2020 protocol and enhancing it with the prismAId tool, this review systematically analyzes the recent evolution of cost assessment methodologies using state-of-the-art generative AI. The AI-enhanced approach facilitates rapid and replicable research extensions. The analysis reveals a significant geographical and sectoral disparity in research on climate adaptation costs, with notable underrepresentation of crucial areas and sectors that are most vulnerable to climate impacts. The study also highlights a predominant reliance on secondary data and a lack of comprehensive uncertainty quantification in economic assessments, suggesting an urgent need for methodological enhancements. It concludes that extending analyses beyond merely verifying that benefits exceed costs is crucial for supporting effective climate adaptation. By assessing the profitability of adaptation investments, it becomes possible to prioritize these investments not only against similar interventions but also across the broader spectrum of public spending.
Tribological Properties of CrN/DLC and CrN Coatings under Different Testing Conditions
Authors: Zhang Shuling , Yang Xiangdong , Huang Tenglong , Guo Feng , Dai Longjie , Liu Yi , Zhang Bo
Published: August 2024

CrN and diamond-like carbon (DLC) coatings are deposited on the surface of 431 stainless steel by the direct current magnetron sputtering technique. The surface morphology, micro-structure, hardness, friction, and wear properties of CrN, CrN/DLC and multi-layer composite DLC coatings are investigated by scanning electron microscopy, X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy, nanoindentation tester, scratch tester, and friction and wear tester. The results show that the surface of the single CrN coating is very rough for the columnar crystal structure with preferred orientation. When it serves as inner transition layers to form the composite DLC coatings, the surface gets much smoother, with reduced defects. The friction and wear results indicate that the composite DLC coatings exhibit lower coefficients of friction, and better wear and corrosion resistance in dry friction, deionized water, and seawater. In the dry wear and friction process, the single CrN coating is easily worn out, and severe friction oxidation and furrow wear both appear with a friction coefficient of 0.48. But the friction coefficient of a CrN coating in seawater is reduced to 0.16, and friction oxidation and wear loss are further reduced with water lubrication. The CrN/DLC coating has excellent tribological performance in three test concoctions and has the lowest friction coefficient of 0.08 in seawater, which is related to the higher sp3 bond content, density (1.907 g/cm3) and high degree of amorphization, contributing to high hardness and a self-lubrication effect. However, due to the limited thickness of CrN/DLC (1.14 µm), it easily peels off and fails during friction and wear in different testing conditions. In multi-layer composite DLC coatings, there are more sp2 bonds with decreased amorphization, high enough thickness (4.02 µm), and increased bonding strength for the formation of different carbides and nitrides of chromium as transition layers, which gives rise to the further decreased average friction coefficient and the lowest wear loss. Therefore, the CrN coating alone has good wear resistance, and, as with the inner transition layer with a DLC coating, it can effectively improve the overall thickness and the bonding strength of the multi-layer films by optimizing the chemical compounds of DLC coatings. These results provide experimental support and reference for the design and selection of surface coatings for 431 stainless steels in different working conditions.