Optimizing the use of the PHYTOTOXKIT test to assess the toxicity of soil contaminated with creosote
Authors: Kucaj Wojciech Filip , Rygielski Kacper , Cybulska Krystyna 
Published: July 2019

<p><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="font-family: TimesNewRomanPSMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">Soil phytotoxicity studies were performed with different doses of creosote by means of<span style="font-family: TimesNewRomanPSMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"> the PHYTOTOXKIT test, using <span style="font-family: TimesNewRomanPS-ItalicMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Sinapis alba</em><span style="font-family: TimesNewRomanPSMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">, <span style="font-family: TimesNewRomanPS-ItalicMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Lepidium sativum</em><span style="font-family: TimesNewRomanPSMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">, <span style="font-family: TimesNewRomanPS-ItalicMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Sorghum saccharatum </em><span style="font-family: TimesNewRomanPSMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">as test<span style="font-family: TimesNewRomanPSMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"> plants. The obtained results indicate highly signifiant effect of the creosote dose, duration of soil<span style="font-family: TimesNewRomanPSMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"> incubation, type of test plant and period, after which the root length measurement was performed<span style="font-family: TimesNewRomanPSMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"> during the phytotoxicity index root test. The analysis of results indicates the highest sensitivity of<span style="font-family: TimesNewRomanPS-ItalicMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em> Sorghum saccharatum </em><span style="font-family: TimesNewRomanPSMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">to creosote and the highest correlation of results obtained with the aid of<span style="font-family: TimesNewRomanPS-ItalicMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em> Lepidium sativum </em><span style="font-family: TimesNewRomanPSMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">when measuring the root length after the fist day the seeds are lined with the<span style="font-family: TimesNewRomanPSMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"> size of the dose. The proposed mathematical model makes it possible to predict the reaction of test<span style="font-family: TimesNewRomanPSMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"> plants on the size of creosote dose as well as to assess its amount in the soil based on the root phytotoxicity. These results allow for a signifiant simplifiation of the test and shorten its duration.<span style="font-family: TimesNewRomanPSMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"> This allows the modifid test to be used for simple monitoring of not only the phytotoxicity but<span style="font-family: TimesNewRomanPSMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"> also the creosote residues during reclamation of contaminated soil.</span></span></span></span></span></span></span></span></span></span><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /><br class="Apple-interchange-newline" /></span></span></span></span></span></span></span></span></span></span></span></p>