bluuebunny/crossref_metadata_2025 huggingface dataset:
PREVALENCE OF ANEMIA AMONG PREGNANT WOMEN A CROSS SECTIONAL STUDY
Authors: Lekhala Mukesh , Acharya Rekha
Published: August 2021

Background: Anemia in pregnancy is a worldwide problem, but it is most prevalent in the developing world. Anemia during pregnancy affects both mother’s and her child’s health. Globally, the prevalence of anemia is 74% among pregnant women, and in India, it ranges from 19% to 61%. Anemia during pregnancy will lead to consequences such as fetal anemia, low birth weight, preterm delivery, intrauterine growth restriction, and perinatal mortality. Anemia during pregnancy and its consequences can be prevented and treated if diagnosed on time.

Methods: A cross-sectional study was conducted at maternity wards of P B M hospital, attached to S P Medical College, Bikaner. The study was conducted for a period of 2 months. Women aged 15-49 yrs. Who had undergone delivery and having ANC record were include in the study after taking informed consent for participation in the study. The data were collected using semi?structured questionnaire.

Results: Out of 1090 women 91.3% women were anemic. Prevalence of anemia was higher(95.3%) in women with less than 3 ANC visit as compare to women with 3 or more ANC visit.

Conclusion: The prevalence of anemia among pregnant women was high in our study and also seen is various studies in different parts of the country. This continuing high burden of anemia could be mainly due to lack of utilization of the services provided by the government

Keywords: Anemia; Hemoglobin Status; Pregnant Women
Strategi Pengelolaan Wakaf Meraih Kepercayaan Umat, Menuju Optimalisasi Pengumpulan: Studi Kasus Badan Wakaf Al-Quran
Authors: Rudianto Sofiyan , Ayuniyyah Qurroh , Supriyanto Trisiladi
Published: August 2021

Penelitian ini bertujuan untuk menggali strategi pengelolaan wakaf untuk meraih kepercayaan masyarakat dan mengoptimalkan penghimpunan dana dengan mengambil kasus Badan Wakaf Al-Qur’an (BWA). Penelitian ini menggunakan metode penelitian kepustakaan. Ini menggunakan data primer dan sekunder. Yang pertama diperoleh dari proses observasi sedangkan yang kedua diperoleh dari situs webnya. Setelah mengkaji strategi pengelolaan BWA, penelitian ini mencoba mengeksplorasi strategi pengelolaan wakaf berdasarkan proses bisnis pengelolaan wakaf. Penelitian ini menyarankan bahwa salah satu faktor kunci keberhasilan pengelolaan wakaf adalah Nazhir (pengelola wakaf). Oleh karena itu, ada beberapa karakter utama yang harus dimiliki oleh Nazhir. Diantaranya adalah iman, taqwa kepada Allah SWT, amanah, dan profesional. Nazhir harus menguasai pengetahuan tentang wakaf, memiliki kepemimpinan yang kuat, dan meningkatkan pengalaman mereka dalam pengelolaan dan promosi lembaga. Dengan pembenahan karakter tersebut, diharapkan para nazir dapat memperoleh kepercayaan dari masyarakat sehingga penghimpunan wakaf dapat lebih optimal.
Провокація кримінального правопорушення під час проведення контролю за вчиненням злочину: етико-правовий аспект
Authors: Аббасова Д. Л.
Published: August 2021

Статтю присвячено дослідженню проблем провокації кримінального правопорушення при проведенні контролю за вчиненням злочину у розрізі етичних аспектів взаємодії суб’єктів кримінального провадження. Автором здійснено аналіз вітчизняної судової практики на предмет виявлення провокації кримінального правопорушення за всіма розділами Особливої частини Кримінального кодексу України. Дослідження проведено на прикладі найчисленнішої, за виявленням провокації з боку органів досудового розслідування, категорії справ – сфері обігу наркотичних засобів, психотропних речовин, їх аналогів або прекурсорів. При цьому особливу увагу приділено морально-етичним аспектам недопустимості використання доказів, здобутих під час проведення негласних (слідчих) розшукових дій, які мають ознаки провокації, а саме ризикам: штучного збільшення показників розкриваємості кримінальних правопорушень і притягнення до кримінальної відповідальності завідомо невинних осіб; негативного впливу на правосвідомість та правову культуру суспільства, включаючи деформацію професійної правосвідомості у формі правового нігілізму. У статті наведено дефініцію провокації кримінального правопорушення та критерії, за яких можливо констатувати наявність або відсутність цього явища з боку органів досудового розслідування, що сформовані практикою Європейського Суду з прав людини. Разом із цим виділено ознаки провокації кримінального правопорушення в матеріальному (змістовному) (ознаки, що вказують на провокування: відсутність підстав для проведення негласних слідчих (розшукових) дій, активна поведінка органів досудового розслідування, яка не має характеру приєднання) та процесуальному (можливість суду перевірити обставини, викладені в заяві про провокацію) аспектах, які знайшли своє відображення у вітчизняній судовій практиці, на підтвердження чого наведено низку судових рішень. До того ж, у статті визначено причини виникнення провокації кримінального правопорушення та запропоновано способи подолання цього негативного явища шляхом криміналізації провокації кримінального правопорушення на прикладі законодавства США, Іспанії, Франції, Німеччини і Грузії. Проблеми, які порушуються у статті, є актуальними та такими, що викликані потребами юридичної науки та практики.
Identifikasi Cendawan Patogen pada Beberapa Kultivar Benih Kacang Tunggak (Vigna unguicullata L.) Berdasarkan Ketinggian Tempat yang Berbeda di Kabupaten Timor Tengah Utara
Authors: Monemnasi Elisabeth Buik
Published: August 2021

Tanaman kacang tunggak (Vigna unguiculata L.) merupakan salah satu tanaman leguminose. Secara fisiologis, tanaman ini memiliki tingkat adaptasi yang cukup tinggi, baik didataran rendah maupun tinggi sehingga menjadi tradisi bagi masyarakat Timor Tengah Utara (TTU) untuk terus membudidayakannya. Hasil produksi kacang tunggak di daerah TTU setiap tahun tetap saja rendah oleh karena kondisi lingkungan dan serangan cendawan patogen pada proses penyimpanan yang kurang tepat. Penelitian ini bertujuan untuk mengetahui jenis-jenis cendawan patogen yang menyerang, ketahanan Kultivar dan pengaruh ketinggian tempat terhadap aktivitas patogen. Penelitian ini dilaksanakan pada bulan September sampai Oktober 2019 di Laboratorium Fakultas Pertanian dan Laboratorium Biologi Universitas Timor. Rancangan penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 2 pola faktorial, faktor pertama Kultivar benih dan faktor kedua adalah daerah. Setiap perlakuan diulang sebanyak 3 kali sehingga terdapat 27 satuan percobaan. Pengamatan identifikasi patogen dilakukan dengan karakterisasi morfologi, dan pengujian vigor viabilitas benih dilakukan dengan metode UKDdp. Data hasil pengamatan kemudian dianalisis dengan menggunakan sidik ragam (anova) rancangan acak lengkap (RAL) dan diuji lanjut dengan menggunakan Duncan Multiple Range Test (DMRT) dengan tingkat signifikan 5 %. Berdasarkan penelitian yang telah dilaksanakan, bahwa terdapat lima cendawan kontamin yang mengkontaminasi benih kacang tunggak Kultivar merah, putih, hitam dari beberapa daerah di wilayah TTU antara lain Cendawan Fussarium sp, Cendawan Melanospora sp, Cendawan Collectotrichum sp, Cendawan Aspergillus sp, dan Cendawan Altenaria sp. Serangan terbesar disebabkan oleh cendawan Fussarium sp.
Pelatihan Terapi Air Terhadap Konstipasi Masa Kehamilan Pada Bidan Di Puskesmas Langsa Barat Kota Langsa
Authors: Henniwati Henniwati , Dewita Dewita
Published: August 2021

ABSTRAK Tenaga kesehatan khusunya bidan sanggat penting untuk meningkatkan pengetahuan dan keterampilan. Untuk meningkatkan itu semua diperlukannya pelatihan-pelatihan khususnya kebidanan. Pelatihan merupakan suatu proses dimana seseorang mencapai kemampuan tertentu. Konstipasi yang sering terjadi selama kehamilan yaitu konstipasi. Prevalensi sembelit pada kehamilan berkisar antara 11% sampai 44%. Salah satu terapi untuk mengatasi konstipasi adalah dengan terapi air. Tujuan kegiatan ini untuk menambah pengalaman, pengetahuan dan kompetensi dalam pemberian asuhan kebidanan terhadap mengurangi konstipasi pada ibu hamil. Pelatihan diberikan selama 3 hari kepada bidan desa, diberikan selama 2 jam. Pengetahuan dan keterampilan bidan menjadi lebih baik setelah mendapatkan edukasi da pelatihan tentang terapi air terhadap konstipasi pada ibu hamil. Kata Kunci  : Pelatihan, Terapi Air, Konstipasi  ABSTRACT  Health Workers, especially midwives, it is very important to increase knowledge and skills. To improve it all requires training, especially midwifery. Training is a process by which a person reaches certain abilities. Constipation that often occurs during pregnancy is constipation. The prevalence of constipation in pregnancy ranges from 11% to 44%. One of the therapies to treat constipation is water therapy. The purpose of this activity is to increase experience, knowledge, and competence in providing midwifery care to reduce constipation in pregnant women. Training is given for 3 days to village midwives, given for 2 hours. The knowledge and skills of midwives became better after receiving education and training on water therapy for constipation in pregnant women. Keywords: Training, Water Therapy, Constipation
Penerapan Terapi Aktivitas Kelompok (TAK) Dan Senam Lansia Sebagai Upaya Pengendalian Demensia Pada Lansia
Authors: Sigalingging Ganda , Nasution Zulkarnain , Pakpahan Hetti Marlina , Tafonao Nasilia
Published: August 2021

ABSTRAK Ancaman triple burden merupakan tantangan yang harus dihadapi di Indonesia, diantaranya jumlah lansia yang terus meningkat. Seiring pertambahan usia, berpotensi menimbulkan masalah kesehatan fisik dan psikososial antara lain depresi, harga diri rendah, bahkan bunuh diri, jika tidak ditangani dengan serius. Oleh karena itu, diperlukan upaya mempertahankan kesehatan lansia pada taraf kesehatan optimal. Tujuan untuk  meningkatkan kemampuan lansia berinteraksi dalam rangka pencapaian penyesuaian psikologi, perilaku dan pencapaian adaptasi optimal.  Metode kegiatan melakukan senam sehat dan terapi aktivitas kelompok (TAK). Hasil kegiatan menunjukkan, secara keseluruhan lansia terlihat ceriah dan bahagia. Disimpulkan kegiatan ini mampu meningkatkan semangat dan menciptakan kebersamaan sesama lansia selama berada di rumah Jompo. Kepada Yayasan panti jompo untuk memfasilitasi kegiatan sesuai kebutuhan lansia yaitu melakukan terapi aktivitas kelompok (TAK) dengan senam sehat secara berkesinambungan untuk  tercipta perasaan yang riang gembira sehingga kualitas hidup lansia  tetap sehat, mandiri, berguna, dan produktif. Kata kunci : Terapi aktivitas kelompok, senam sehat, demensia,  lansia.    ABSTRACT The triple threat is a challenge that must be faced in Indonesia. More and more the number of elderly continues to increase. As you age, changes occur due to the aging process that causes physical and psychosocial problems. one of the psychosocial problems in the elderly is low self-esteem. If that doesn't work, the elderly will repair, attract, challenge, and even escape. Therefore, it is necessary to maintain the health of the elderly at an optimal level of health. The goal is to increase the ability of the elderly in the contexts of psychological loss, behavior, and optimal adaptation. Activity methods of doing healthy gymnastics and group activity therapy. The results of the activity showed that overall the elderly looked bright and happy.  This activity can increase enthusiasm and create togetherness among the elderly in a nursing home.  Keywords: Group activity therapy, healthy gymnastics, dementia, elderly.
Pengabdian Masyarakat di Wilayah Kerja puskesmas Pancu Batu Kabupaten Deliserdang
Authors: Sigalingging Vina Yolanda Sari , Simanullang Murni Sari Dewi , Simorangkir Lindawati , Barus Mardiati , Pakpahan Rotua Elvina , Tampubolon Lindawati F , Derang Imelda
Published: August 2021

ABSTRAK Untuk mencapai kesehatan yang panjang harus dimulai sejak dini. Pada usia dini, balita sangatlah berperan penting sebagai permulaan kehidupan yang akan datang. Tindakan kesehatan yang dapat dilakukan diawal kehidupan manusia adalah dengan adanya imunisasi. Imunisasi yang di berikan haruslah lengkap hingga batas usia dengan berbagai macam imunisasi yang akan diterima. Tujuan dari pengabdian masyarakat ini adalah untuk meningkatkan kesehatan balita sejak dini. Sasaran kegiatan ini adalah bayi dibawah umur 5 tahun yang akan menerima imunisasi. Hasil dari kegiatan ini adalah balita yang dating mendapatkan imunisasi sesuai dengan umurnya. Diharapkan dengan dilaksanakannya pengabdian masyarakat ini, para ibu dapat rajin untuk membawa balita mereka untuk di bawa keposyandu secara rutin dan balita mendapatkan imunisasi lengkap guna memberikan kesehatan yang baik di masa yang akan dating dan mengurangi resiko sakit, cacat dan kematian pada balita. Kata Kunci :Posyandu, Balita ABSTRACT To achieve long health must start early. At an early age, toddlers are very important as the beginning of life to come. Health action that can be done early in human life is immunization. The immunization given must be complete up to the age limit with a variety of immunizations to be received. The purpose of this community service is to improve the health of toddlers from an early age. The target of this activity is infants under 5 years of age who will receive immunizations. The result of this activity is toddlers who come to receive immunizations according to their age. It is hoped that with the implementation of this community service, mothers can be diligent in bringing their toddlers to the Integrated Healthcare Center, regularly and toddlers get complete immunizations to provide good health in the future and reduce the risk of illness, disability and death in toddlers. Keywords: Integrated Healthcare Center, Toddler
Pemberdayaan Keluarga Melalui Pendampingan Budidaya “Empon-Empon” Serta Produksi Dan Branding Karak Non “Bleng”
Authors: Pangestu Lila , Nur Annisa Syarah Ayu
Published: August 2021

Pandemi Corona Virus 19 berdampak terhadap keseluruhan aspek kehidupan , khususnya ketahanan ekonomi keluarga dengan ditandai adanya menurunnya produksi, distribusi, pendapatan dari home industry Tujuan kegiatan ini adalah memberdayakan  keluarga melalui budidaya empon- empon serta membranding  karak non “bleng  berbasis kearifan lokal di Dusun Nglaroh, Nangsri, Kebakkramat, Karanganyar. Metode pendekatan pada kegiatan ini adalah rural development and empowerment melalui penyampaian materi, diskusi, latihan praktik (drill practice) serta pendampingan. Sasaran kegiatan ini adalah kelompok produktif ekonomi yang terdiri dari 10 home industri dan kelompok masyarakat non produktif ekonomi yang berjumlah 30 keluarga. Indikator kegiatan ini adalkah peningkatan pengetahuan dan ketrampilan dalam budidaya empon – empon dan pengetahuan dan keteampilan dalam pengelolaan kerak non bleng sebagai produk unggulan serta partisipasi peserta dalam kegiatan. Instrument yang dipakai adalah ceklist pre dan post. Adapun hasil kegiatan ini adalah adanya peningkatan pengatahuan, ketrampilan serta partisipasi masyarakat terhadap aspek kegiatan ini sebesar 90%. Ketahanan ekonomi keluarga dapat diperbaiki melalui pemberdayaan kelompok degan budidaya empon- embpon dan branding kerak belng pada masyarakat ekonomi produktif dan non preoduktif di desa Ngangsi Kebak kramat Karanganyar Jawa Tengah
Thin layer chromatographic detection of enrofloxacin antibiotic residues in poultry tissues
Authors: Islam Bhuiyan Md Mominul , Islam Md Shafiqul , Hasan Md Rakibul , Islam Kazi Rafiqul
Published: August 2021

Antibiotic residues remain in edible portion of meat animals that have been treated with antibiotics. The aim of this study was to detect enrofloxacin residue after discriminate and indiscriminate administration and investigate the effect of enrofloxacin in growth of poultry. 18 broilers DOC (Cobb-500) were collected & reared up to 31 days. On day 16, they were randomly divided into 3 groups, namely Group–A (Control group), Group-B (Discriminate group) and Group-C (Indiscriminate group). Each group contains 6 birds. The discriminate and indiscriminate groups were treated with antibiotic, enrofloxacin. In Group-B withdrawal period was followed and treatment was stopped before 7 days of sacrifice. On the other hand, withdrawal period was not maintained in indiscriminate group and antibiotic treatment was continued until the day of sacrifice. Body weight was recorded daily in the morning. On 31st day mean body weight was highest in Group-C (1901.17 ± 15.22gm) and lowest body weight was in Group-A (1453.33 ± 26.39gm). The differences among mean weight gain were statistically significant (P<0.005) in both discriminate & indiscriminate group compared to control group. Test results found in TLC showed that in discriminate antibiotic group (Group-B) 50% liver samples, 33.33% kidney and 16.67% fat samples were enrofloxacin positive. No sample of thigh muscle, breast muscle and spleen was positive. In indiscriminate antibiotic group (group-C) all the samples were positive in case of liver, kidney, fat and spleen samples. Only 33.33% and 16.67 % samples were positive in case of fat and thigh muscle samples. All the samples of control group (Group-A) were negative. Overall, the present study documented the widespread abuse of enrofloxacin and failure to implement the recommended withdrawal period will undeniably leads to deposition of residues in broiler tissues.
Asian Australas. J. Food Saf. Secur. 2021, 5 (1), 11-18
Quality characteristics and hatchability of eggs and serum electrolytes concentration of Gola, Giribaz and Ghiachundi pigeon breed
Authors: Sujan Khaled Mahmud , Biswas Manik , Tareq Sakhawat Hossain , Islam Md Kamrul
Published: August 2021

The weather and vast areas of crop fields along with housing premises of Bangladesh are suitable for pigeon farming. The experiment was conducted to evaluate the characteristics of eggs and serum electrolyte concentration of Gola, Giribaz and Ghiachundi pigeon squabs. Three pairs of each breed were reared in the cage with commercial feed and water. After laying eggs, the length and width of eggs were recorded. After hatching of eggs, eggshell thickness was recorded. The thickness of the eggshell was 0.15±0.01 mm, 0.15±0.01 mm, 0.15±0.00 mm for Gola, Giribaz and Ghiachundi breeds respectively. The body weights of the squabs were recorded at 7th and 45th day. The squabs were sacrificed on 45th day and the blood sample was collected and serum sodium, potassium, calcium and phosphorus concentration were measured by using appropriate analytical techniques. Serum sodium, potassium, calcium and phosphorus were: 102.93±15.95 (mmol/L), 11.18±2.19 (mmol/L), 6.20±1.11 (mmol/L), 4.53±0.45 (mmol/L) for Gola breed; 101.48±2.12 (mmol/L), 12.15±0.96 (mmol/L), 5.65±1.09 (mmol/L), 4.53±0.36 (mmol/L) for Giribaz breed and 95.18±6.71 (mmol/L), 11.99±0.91 (mmol/L), 5.09±0.75(mmol/L), 4.81±0.24 (mmol/L) for Ghiachundi breed respectively. A positive correlation has been found between thickness of eggshell and phosphorus while there is a negative correlation with calcium for Gola breed; both have been found insignificant for Giribaz breed and only calcium has been found significant for Ghiachundi breed. A strong correlation between the final body weight of the squab and K for the Gola breed; between the final body weight of the squab and Ca, P, K for Giribaz breed and between the final body weight of the squab and Ca, Na, K have been found. It could be concluded that serum electrolytes having an influential effect on egg characteristics and body weight of corresponding pigeon breed.
Asian J. Med. Biol. Res. 2021, 7 (2), 107-112
Proximate composition, amino acids, and fatty acids contents of dried shrimp products available in Jashore region, Bangladesh
Authors: Das Pranta , Md Salman - , Islam Md Aminur , Suraiya Sharmin , Haq Monjurul
Published: August 2021

Dried shrimp has some special advantageous such as long shelf-life, high nutritional content, and ease of transportation considered as a healthy choice of food. The nutritional properties of three common and demandable dried shrimp species available in Jashore, Bangladesh were evaluated. The moisture content of dried Palaemon karnafuliensis, Metapenaeus Monoceros and Ferapenaeus indicus was determined 19.7±0.40%, 20.5±0.25% and 24.9±0.21%, respectively. The protein content was found 57.46±5.88%, 62.5±1.98%, and 55.5±1.85% in Palaemon karnafuliensis, Metapenaeus Monoceros, and Ferapenaeus indicus, respectively. The ash and fat content of Palaemon karnafuliensis, Metapenaeus Monoceros and Ferapenaeus indicus were observed 12.20±0.90% and 1.90±0.15%, 10.20±0.39% and 1.48±0.32%, 8.57±1.43%, and 1.08±0.21%, respectively. Total saturated fatty acids content was found 31.56%, 29.21%, 38.59 in Palaemon karnafuliensis, Metapenaeus monoceros, and Ferapenaeus indicus, respectively. The polyunsaturated fatty acids was found 42.60%, 42.29%, and 37.80% in Palaemon karnafuliensis, Metapenaeus monoceros, and Ferapenaeus indicus, respectively. There were nine non-essential and eight essential amino acids found in the dried shrimp products. Glutamine, proline, glycine and alanine were dominated among the non-essential amino acid. Lysine was found a significant amount in the study. All the three dried shrimp products were considered as highly nutritive and less fat value which is considered healthy for the consumers.
Asian J. Med. Biol. Res. 2021, 7 (2), 138-146
Genetic diversity analysis of advanced rice lines for salt tolerance using SSR markers
Authors: Singha Tanoy , Mahamud Md Asif , Imran Shahin , Paul Newton Chandra , Hoque Md Najmol , Chakrobarty Tusher , Al Galib Md Asadulla , Hassan Lutful
Published: August 2021

Twenty-two rice lines were used to evaluate salt tolerance at the Laboratory of Biotechnology Division of Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh. Four SSR markers viz. AP3206f, RM1287, RM7075, and RM10793 were used to screen the germplasm for salt tolerance. SSR analysis revealed that the number of alleles per locus ranged from 3 to 5 with an average of 4.25 alleles per locus. Polymorphism Information Content (PIC) values ranged from 0.4762 (RM7075) to 0.7524 (AP3206f) with an average of 0.61 per locus. The highest genetic diversity (0.7810) was observed in loci AP3206f, and the lowest genetic diversity (0.5620) was observed in loci RM7075 with a mean diversity of 0.6663. The genotypes with genetic similarity clustered together in the dendrogram based on UPGMA method and we observed seven major clusters where cluster I contained most of the genotypes. Cluster I, II, III, IV, V, VI, and VII contained 6, 1, 2, 4, 4, 4 and 1 genotypes, respectively. These results revealed that marker AP3206f would be best in screening 22 rice genotypes followed by RM1287, RM7075, and RM10793 according to PIC values. These findings can have the potential role for further improvement of salinity tolerance rice genotypes through marker-assisted breeding.
Asian J. Med. Biol. Res. 2021, 7 (2), 214-221
Pengembangan Model Konseptual Niat Pembayaran Pajak Pelaku E-Commerce
Authors: Dewi Sari
Published: August 2021

Ecommerce transactions are one of the complex and growing types of transactions in Indonesia. Ecommerce trade is currently experiencing very rapid progress which is marked by a growing improvement in current technological advances, especially in the field of e-commerce. The largest state revenue can be seen from the 2019 State Revenue and Expenditure Budget of state revenues of 1,786.4 trillion rupiahs from taxes. The Ministry of Finance enforces the Minister of Finance Regulation Number 210/PMK.010/2018 concerning Tax Treatment of Trading Transactions Through Electronic Systems. In response to this, there is the potential for an exodus of e-commerce players to switch to using social media as a place for online buying and selling transactions. The payment intention factor for e-commerce transactions is based on three aspects, namely, knowledge and understanding of taxation. Internal influences in the form of taxpayer awareness, ethics, the use of information technology which is influenced by the external readiness of information technology, as well as the influence of the taxpayer's environment. This study aims to propose a conceptual model to identify the factors that influence the tax payment intention of e-commerce actors. This research is expected to provide suggestions to the Directorate General of Taxes regarding e-commerce taxpayers.
Cardiotoxicidad en una Cohorte de pacientes con Diagnóstico de Cáncer de Mama que Recibieron Quimioterapia con Antraciclinas. Seguimiento a 12 años
Authors: Santacruz Maridueña Mayra Patricia , García Matamoros EDITOR: Dra. Katherine
Published: August 2021

Introducción: Los esquemas de quimioterapia en el tratamiento de cáncer de mama incluyen Antraciclinas con una efectividad de respuesta alta, sin embargo en algunos casos el potencial efecto terapéutico se ve limitado por la Cardiomiopatía Inducida por Antraciclinas (CIPA). El objetivo del estudio fue establecer la prevalencia longitudinal de esta entidad.

Métodos: Este estudio observacional, longitudinal fue realizado en el Instituto Oncológico Nacional “Dr. Juan Tanca Marengo” -SOLCA-Guayaquil. El período de exposición fue abril a diciembre del 2008. El período de observación terminó el 30 de junio del 2020. Incluyó mujeres >18 años con cáncer de mama, en tratamiento con antraciclinas, clasificadas por riesgo Cardiovascular de Framingham en Riesgo Bajo (A), riesgo moderado (B) y riesgo alto (C). Se midió las Alteraciones electrocardiográficas (AEKG) basales, a las 6 horas y a las 24 horas y desarrollo de CIPA en seguimiento a 12 años. La muestra fue no probabilística tipo censo. Se utiliza estadística descriptiva con Intervalo de confianza para proporciones.

Resultados: Ingresaron al estudio 50 casos son AEKG. El desarrollo de AEKG a 6 horas en 18/50 casos (36% IC95% 34.1-37.9%), estas AEKG persistieron hasta las 24 horas. Las AEKG se presentaron en un 18.52% en mujeres con Riesgo A, 52.38% en Riesgo B y 100% en Riesgo C. El desarrollo de CIPA  a 12 años fue de 3.57% (2.27-4.87%) en CIPA-Subaguda y de 7.14% (5.34-8.95% en CIPA crónica. CIPA Subaguda+ crónica 10.71% (8.55-12.88%).

Conclusiones: En este estudio se concluye que los eventos de cardiotoxicidad aguda fueron detectados por cambios electrocardiográficos y que en el seguimiento a largo plazo fueron evidentes en un porcentaje un poco mayor a la reportada en la literatura internacional.
GAMBARAN PENGETAHUAN DAN SIKAP PERAWAT RUANG OK DALAM PEMBERIAN INISIASI MENYUSU DINI (IMD) DI RSUD Dr. HADRIANUS SINAGA PANGURURAN KABUPATEN SAMOSIR TAHUN 2019
Authors: Marpaung Ricard Fredrik , Derlina Rostio
Published: August 2021

Tujuan penelitian ini adalah mengetahui gambaran pengetahuan dan sikap perawat ruang OK dalam pemberian inisias menyusui dini (IMD) di RSUD Dr. Hadrianus Sinaga Pangururan Kabupaten Samosir Tahun 2017. Jenis penelitian ini adalah cross-sectional. Populasi dalam penelitian ini adalah seluruh perawat ruang OK RSUD Dr. Hadrianus Sinaga Pangururan Kabupaten Samosir yaitu sebanyak 10 orang. Data dikumpulkan melalui pengisian kuesioner dan dianalisis secara deskriptif berupa frekuensi dan persentase. Didapatkan bahwa: 1) mayoritas mempunyai pengetahuan cukup yaitu sebanyak 4 orang (40%) sedangkan minoritas mempunyai pengetahuan baik yaitu sebanyak 3 orang (30%) dan pengetahuan kurang yaitu sebanyak 3 orang (30%), 2) mayoritas mempunyai sikap setuju yaitu sebanyak 7 orang (70%) sedangkan minoritas sikap tidak setuju yaitu sebanyak 3 orang (30%).

Kata kunci: inisiasi menyusu dini (IMD); pengetahuan; sikap
Sosialisasi Pesantren dan Santri Sehat di Pondok Pesantren Mahasiswa Liwa’ul Haq
Authors: Hafid Fahmi , Nasrul Nasrul , Zainul Zainul , Junaidi Junaidi , Hartini Diah Ayu
Published: August 2021

Pesantren telah menjadi Lembaga Pendidikan Islam yang tertua, unik dan terus berkembang. Dalam sejarah, pesantren berkiprah turut membangun bangsa Indonesia. Karena peran strategisnya itulah, pesantren dapat menjadi fondasi yang kuat bagi penggalakan gerakan masyarakat hidup sehat terutama bagi santri dan pengurusnya. Tujuan pengabdian masyarakat ini adalah terlaksananya kegiatan Sosialisasi Pesantren dan Santri Sehat Pesantren Mahasiswa Liwa’ul Haq Kota Palu. Metode pelaksanaan dengan Sosialisasi Pesantren dan Santri Sehat kepada 20 santri dan santriwati serta pengurus pesantren. Pelaksanaan pada tanggal 11-12 Februari 2021 di Pondok Pesantren Mahasiswa Liwa’ul Haq Kelurahan Tondo Kecamatan Mantikulore Kota Palu Sulawesi Tengah. Hasil yang diperoleh dari kegiatan sosialisasi ini adalah terjalinnya kerjasama antara Poltekkes Kemenkes Palu dengan Pesantren Mahasiswa Liwa’ul Haq dalam rangka pembentukan Pos Kesehatan Pesantren (Poskestren) dan pembinaan pesantren dan santri sehat di Kota Palu.
Chryseobacterium aquifrigidense FANN1 Produced Detergent-Stable Metallokeratinase and Amino Acids Through the Abasement of Chicken Feathers
Authors: Bokveld Amahle , Nnolim Nonso E. , Nwodo Uchechukwu U.
Published: August 2021

Microbial keratinases’ versatility in the beneficiation of keratinous waste biomass into high-value products prompts their application in diverse spheres hence, advancing green technology and the bioeconomy. Consequently, a feather-degrading Chryseobacterium aquifrigidense FANN1 (NCBI: MW169027) was used to produce keratinase, and its biochemical properties were determined. The optimization of physicochemical parameters and analysis of the free amino acid constituents of the feather hydrolysate were also carried out. FANN1 showed a maximum keratinase yield of 1,664.55 ± 42.43 U/mL after 72 h, at optimal process conditions that included initial medium pH, incubation temperature, inoculum size, and chicken feather concentration of 8, 30°C, 4% (v/v), and 15 (g/L), respectively. Analysis of degradation product showed 50.32% and 23.25% as the protein value and total free amino acids, respectively, with a relatively high abundance of arginine (2.25%) and serine (2.03%). FANN1 keratinase was optimally active at pH 8.0 and relatively moderate to high temperature (40–50°C). EDTA and 1,10-phenanthroline inhibited the keratinase activity, and that suggests a metallo-keratinase. The enzyme showed remarkable stability in the presence of chemical agents, with residual activity 141 ± 10.38%, 98 ± 0.43%, 111 ± 1.73%, 124 ± 0.87%, 104 ± 3.89%, 107 ± 7.79%, and 112 ± 0.86% against DTT, H2O2, DMSO, acetonitrile, triton X-100, tween-80, and SDS, respectively. The residual activity of FANN1 keratinase was enhanced by Sunlight (129%), Ariel (116%), MAQ (151%), and Surf (143%) compared to the control after 60 min preincubation. Likewise, the enzyme was remarkably stable in the presence Fe3+ (120 ± 5.06%), Ca2+ (100 ± 10.33%), Na+ (122 ± 2.95%), Al3+ (106 ± 10.33%); while Co2+ (68 ± 8.22%) and Fe2+ (51 ± 8.43%) elicited the most repressive effect on keratinase activity. The findings suggest that C. aquifrigidense FANN1 is a potential candidate for keratinous wastes bio-recycling, and the associated keratinase has a good prospect for application in detergent formulation.
Androgen Activity Is Associated With PD-L1 Downregulation in Thyroid Cancer
Authors: O’Connell Timmy J. , Dadafarin Sina , Jones Melanie , Rodríguez Tomás , Gupta Anvita , Shin Edward , Moscatello Augustine , Iacob Codrin , Islam Humayun , Tiwari Raj K. , Geliebter Jan
Published: August 2021

Thyroid cancer is the most prevalent endocrine malignancy in the United States with greater than 53,000 new cases in 2020. There is a significant gender disparity in disease incidence as well, with women developing thyroid cancer three times more often than men; however, the underlying cause of this disparity is poorly understood. Using RNA-sequencing, we profiled the immune landscape of papillary thyroid cancer (PTC) and identified a significant inverse correlation between androgen receptor (AR) levels and the immune checkpoint molecule PD-L1. The expression of PD-L1 was then measured in an androgen responsive-thyroid cancer cell line. Dihydrotestosterone (DHT) treatment resulted in significant reduction in surface PD-L1 expression in a time and dose-dependent manner. To determine if androgen-mediated PD-L1 downregulation was AR-dependent, we treated cells with flutamide, a selective AR antagonist, and prior to DHT treatment to pharmacologically inhibit AR-induced signaling. This resulted ina> 90% restoration of cell surface PD-L1 expression, suggesting a potential role for AR activity in PD-L1 regulation. Investigation into the AR binding sites showed AR activation impacts NF-kB signaling by increasing IkBα and by possibly preventing NF-kB translocation into the nucleus, reducing PD-L1 promoter activation. This study provides evidence of sex-hormone mediated regulation of immune checkpoint moleculesin vitrowith potential ramification for immunotherapies.
Chondroitin Sulfate/Dermatan Sulfate-Protein Interactions and Their Biological Functions in Human Diseases: Implications and Analytical Tools
Authors: Zhang Bin , Chi Lianli
Published: August 2021

Chondroitin sulfate (CS) and dermatan sulfate (DS) are linear anionic polysaccharides that are widely present on the cell surface and in the cell matrix and connective tissue. CS and DS chains are usually attached to core proteins and are present in the form of proteoglycans (PGs). They not only are important structural substances but also bind to a variety of cytokines, growth factors, cell surface receptors, adhesion molecules, enzymes and fibrillary glycoproteins to execute series of important biological functions. CS and DS exhibit variable sulfation patterns and different sequence arrangements, and their molecular weights also vary within a large range, increasing the structural complexity and diversity of CS/DS. The structure-function relationship of CS/DS PGs directly and indirectly involves them in a variety of physiological and pathological processes. Accumulating evidence suggests that CS/DS serves as an important cofactor for many cell behaviors. Understanding the molecular basis of these interactions helps to elucidate the occurrence and development of various diseases and the development of new therapeutic approaches. The present article reviews the physiological and pathological processes in which CS and DS participate through their interactions with different proteins. Moreover, classic and emerging glycosaminoglycan (GAG)-protein interaction analysis tools and their applications in CS/DS-protein characterization are also discussed.
Application of the Stacked Refraction Convolution Section to 2D Ocean Bottom Seismometer Wide-angle Seismic Data Along the Tamayo Through Basin, Gulf of California
Authors: González-Fernández Antonio
Published: August 2021

The stacked refraction convolution section can be used as an interpretation tool in wide-angle refraction seismic data generated by air gun shooting and recorded by Ocean Bottom Seismometers (OBS). The refraction convolution section is a full-wave extension of the Generalized Reciprocal Method (GRM), a method frequently used in shallow refraction seismic interpretation, but not applied to deep crustal-scale studies. The sum of the travel times of the waves refracted in the same interface and recorded in a pair of forward and reverse profiles, time-corrected by the reciprocal time, is an estimation close to the two-way travel times of the multichannel seismic reflection sections, but with seismic rays illuminating the interfaces upwards. The sum of seismic traces is obtained with the convolution section. Furthermore, several pairs of convolved forward-reverse refraction recordings of the same area can be stacked together to improve the signal to noise ratio. To show the applicability of the refraction convolution section in OBS deep data, we interpreted the basement structure of the Tamayo Through Basin in the southern Gulf of California, offshore Mexico. We compared the results with both, a multichannel seismic section recorded in the same profile, and the previous interpretations of the same wide-angle seismic data modeled with ray tracing and tomography methods. The basement imaged by the stacked refraction convolution section is similar in geometry to that obtained by seismic reflection processing. The stacked refraction convolution section identifies the full extent of the basement and confirms the location of a nearly constant thickness volcanic layer in the northwestern half of the basin. However, only a small area of volcanic deposits is found in the shallower parts of the southwestern margin. We also show that the convolution process can be used to estimate the occurrence of lateral variations of seismic velocities in the basement, as a further application of the GRM to deep refraction data.
Nomogram Model to Predict the Probability of Ovarian Hyperstimulation Syndrome in the Treatment of Patients With Polycystic Ovary Syndrome
Authors: Li Fei , Chen Ying , Niu Aiqin , He Yajing , Yan Ying
Published: August 2021

Objective

The objective of this study was to explore the risk factors of ovarian hyperstimulation syndrome (OHSS) in patients with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) and to establish a nomogram model evaluate the probability of OHSS in PCOS patients.

Methods

We retrospectively analyzed clinical data from 4,351 patients with PCOS receiving IVF/ICSI in our reproductive medical center. The clinical cases were randomly divided into a modeling group (3,231 cases) and a verification group (1,120 cases) according to a ratio of about 3:1. The independent risk factors correlation with the occurrence of OHSS was identified by logistic regression analysis. Based on the selected independent risk factors and correlated regression coefficients, we established a nomogram model to predict the probability of OHSS in PCOS patients, and the predictive accuracy of the model was measured using the area under the receiver operating curve (AUC).

Results

Univariate and multivariate logistic regression analyses showed that FSH (OR, 0.901; 95% CI, 0.847–0.958; P<0.001), AMH (OR, 1.259; 95% CI, 1.206–1.315; P<0.001), E2 value on the day of hCG injection (OR, 1.122; 95% CI, 1.021–1.253; P<0.001), total dosage of Gn used (OR, 1.010; 95% CI, 1.002–1.016; P=0.041), and follicle number on the day of hCG injection (OR, 0.134; 95% CI, 1.020–1.261; P=0.020) are the independent risk factors for OHSS in PCOS patients. The AUC of the modeling group is 0.827 (95% CI, 0.795–0.859), and the AUC of the verification group is 0.757 (95% CI, 0.733–0.782).

Conclusion

The newly established nomogram model has proven to be a novel tool that can effectively, easily, and intuitively predict the probability of OHSS in the patients with PCOS, by which the clinician can set up a better clinical management strategies for conducting a precise personal therapy.
Rare Genetic Disorders: Novel Treatment Strategies and Insights Into Human Biology
Authors: Koch Peter J. , Koster Maranke I.
Published: August 2021

The last decade has seen a dramatic increase in innovative ideas for the treatment of genetic disorders for which no curative therapies exist. Gene and protein replacement therapies stand out as novel approaches to treat a select group of these diseases, such as certain tissue fragility disorders. Further, the advent of stem cell approaches, such as induced pluripotent stem cells (iPSC) technology, has led to the development of new methods of creating replacement tissues for regenerative medicine. This coincided with the discovery of genome editing techniques, which allow for the correction of disease-causing mutations. The culmination of these discoveries suggests that new and innovative therapies for monogenetic disorders affecting single organs or tissues are on the horizon. Challenges remain, however, especially with diseases that simultaneously affect several tissues and organs during development. Examples of this group of diseases include ectodermal dysplasias, genetic disorders affecting the development of tissues and organs such as the skin, cornea, and epithelial appendages. Gene or protein replacement strategies are unlikely to be successful in addressing the multiorgan phenotype of these diseases. Instead, we believe that a more effective approach will be to focus on correcting phenotypes in the most severely affected tissues. This could include the generation of replacement tissues or the identification of pharmaceutical compounds that correct disease pathways in specific tissues.
GARP Correlates With Tumor-Infiltrating T-Cells and Predicts the Outcome of Gastric Cancer
Authors: Jiang Sutian , Zhang Yifan , Zhang Xiaojing , Lu Bing , Sun Pingping , Wu Qianqian , Ding Xuzhong , Huang Jianfei
Published: August 2021

Accepting the crucial role of the immune microenvironment (TME) in tumor progression enables us to identify immunotherapeutic targets and develop new therapies. Glycoprotein A repetitions predominant (GARP) plays a vital part in maintaining regulatory T cell (Treg)-mediated immune tolerance. The impact of GARP in TME of gastric cancer is still worth exploring. We investigated public genomic datasets from The Cancer Genome Atlas and Gene Expression Omnibus to analyze the possible role of GARP and its relationship with TME of gastric cancer. Fluorescence-based multiplex immunohistochemistry and immunohistochemistry for T-cell immune signatures in a series of tissue microarrays were used to validate the value of GARP in the TME. We initially found that GARP expression was upregulated in gastric carcinoma cells, and diverse levels o3f immune cell infiltration and immune checkpoint expression were detected. Gene expression profiling revealed that GARP expression was related to the TME of gastric cancer. GARP upregulation was usually accompanied by increased FOXP3+ Treg and CD4+ T cell infiltration. In addition, GARP expression had positive relationships with CTLA-4 and PD-L1 expression in gastric cancer. Cox regression analysis and a nomogram highlighted that the probability of poor overall survival was predicted well by GARP or GARP+CD4+ T cell. Taken together, this research underlines the potential effect of GARP in regulating survival and tumor-infiltrating T-cells. In addition, the function of CD4+ T cell immune signatures in the prognosis can be clinically meaningful, thereby providing a new idea for the immunotherapeutic approach.
sCD28, sCD80, sCTLA-4, and sBTLA Are Promising Markers in Diagnostic and Therapeutic Approaches for Aseptic Loosening and Periprosthetic Joint Infection
Authors: Jubel Jil M. , Randau Thomas M. , Becker-Gotot Janine , Scheidt Sebastian , Wimmer Matthias D. , Kohlhof Hendrik , Burger Christof , Wirtz Dieter C. , Schildberg Frank A.
Published: August 2021

Aseptic prosthetic loosening and periprosthetic joint infections (PJI) are among the most frequent complications after total knee/hip joint arthroplasty (TJA). Current research efforts focus on understanding the involvement of the immune system in these frequent complications. Different immune cell types have already been implicated in aseptic prosthetic loosening and PJI. The aim of this study was to systematically analyze aspirates from knee and hip joints, evaluating the qualitative and quantitative composition of soluble immunoregulatory markers, with a focus on co-inhibitory and co-stimulatory markers. It has been shown that these molecules play important roles in immune regulation in cancer and chronic infectious diseases, but they have not been investigated in the context of joint replacement. For this purpose, aspirates from control joints (i.e., native joints without implanted prostheses), joints with TJA (no signs of infection or aseptic loosening), joints with aseptic implant failure (AIF; i.e., aseptic loosening), and joints with PJI were collected. Fourteen soluble immunoregulatory markers were assessed using bead-based multiplex assays. In this study, it could be shown that the concentrations of the analyzed immunoregulatory molecules vary between control, TJA, AIF, and PJI joints. Comparing TJA patients to CO patients, sCD80 was significantly elevated. The marker sBTLA was significantly elevated in AIF joints compared to TJA joints. In addition, a significant difference for eight markers could be shown when comparing the AIF and CO groups (sCD27, sCTLA-4, sCD137, sCD80, sCD28, sTIM-3, sPD-1, sBTLA). A significant difference was also reached for nine soluble markers when the PJI and CO groups were compared (sLAG-3, sCTLA-4, sCD27, sCD80, sCD28, sTIM-3, sPD-1, IDO, sBTLA). In summary, the analyzed immunoregulatory markers could be useful for diagnostic purposes as well as to develop new therapeutic approaches for AIF and PJI.
Runx Transcription Factors in T Cells—What Is Beyond Thymic Development?
Authors: Korinfskaya Svetlana , Parameswaran Sreeja , Weirauch Matthew T. , Barski Artem
Published: August 2021

Runx proteins (also known as Runt-domain transcription factors) have been studied for a long time as key regulators of cellular differentiation. RUNX2 has been described as essential for osteogenesis, whereas RUNX1 and RUNX3 are known to control blood cell development during different stages of cell lineage specification. However, recent studies show evidence of complex relationships between RUNX proteins, chromatin-modifying machinery, the cytoskeleton and different transcription factors in various non-embryonic contexts, including mature T cell homeostasis, inflammation and cancer. In this review, we discuss the diversity of Runx functions in mature T helper cells, such as production of cytokines and chemokines by different CD4 T cell populations; apoptosis; and immunologic memory acquisition. We then briefly cover recent findings about the contribution ofRUNX1, RUNX2andRUNX3to various immunologic diseases. Finally, we discuss areas that require further study to better understand the role that Runx proteins play in inflammation and immunity.
Ablation of Survivin in T Cells Attenuates Acute Allograft Rejection after Murine Heterotopic Heart Transplantation by Inducing Apoptosis
Authors: Xu Heng , Yu Jizhang , Cui Jikai , Chen Zhang , Zhang Xi , Zou Yanqiang , Du Yifan , Li Yuan , Le Sheng , Jiang Lang , Xia Jiahong , Wu Jie
Published: August 2021

Although studies in oncology have well explored the pharmacological effects of Birc5, little is known about its role in allogeneic T-cell responses. Therefore, the present study used a mouse model of acute heart allograft rejection to investigate the protective effect and mechanism of conditional knockout of Birc5 in T cells. Survivin (encoded by Birc5) was up-regulated in T cells activated in vivo and in vitro. Deletion of Birc5 in T cells attenuated acute heart allograft rejection by reducing the ratio of effector to naive T cells and Th1 to Tregs. In addition, deletion of Birc5 had no noticeable effect on proliferation but on apoptosis and the secretion of IFN-γ. The results revealed a significant increase in the percentage of Annexin V positive CD4+ T cells in the Birc5-/- group, compared to the WT. Moreover, there was significant increase in early apoptotic alloreactive T cells in Birc5-/- mice and this was partly mediated by caspase-3. Furthermore, treatment with YM155 inhibited acute heart allograft rejection in vivo and increased T-cell apoptosis in healthy human PBMCs in vitro. The results highlight a potential therapeutic target for the prevention and treatment of acute transplant rejection.