bluuebunny/crossref_metadata_2025 huggingface dataset:
Short Communication: Evaluation of water quality and survival rate of red tilapia (Oreochromis niloticus) by using rice-fish culture system in quarry land of clay
Authors: HIDAYATI DEWI , NURTJAHYANI SUPIANA DIAN , OKTAFITRIA DWI , ASHURI NOVA MAULIDINA , KURNIALLAH WAHYU
Published: March 2019

Abstract. Hidayati D, Nurtjahyani SD, Oktafitria D, Ashuri NM, Kurniallah W. 2019. Short Communication: Evaluation of water quality and survival rate of red tilapia (Oreochromis niloticus) by using rice-fish culture system in quarry land of clay. Biodiversitas 20: 589-594. Quarry land is an area that has been used as mining land and has different characteristics from the former conditions before being mined so that the level of productivity of the soil decreases. This study aims to determine the suitability of clay quarry land in Tuban as a cultivation medium based on the analysis of the water quality and survival rate of red tilapia (Oreochromis niloticus) using rice-fish culture system. This study used a completely randomized experimental design method with triplicate of three ratio of cultivation medium using Ex-mining Land Soil (EmLS) from quarry land and Top Soil, i.e., 1:0 (A), 1:1 (B), and 0:1 (C/Control). Results of water quality measurements that include nitrite, nitrate, temperature, pH, and dissolved oxygen in descriptive unreal influence showed good condition. However, ammonia gives a significantly different effect mainly on the survival rate in 30 days nurture. In the end, the ANOVA test result of tilapia survival in treatment A was not significantly different (P>0.05) with treatment B and control. Based on these data, it can be seen that the EmLS from the quarry land of clay and TS can be used as a medium for cultivation of red tilapia (O. niloticus) with the recommended ratio of 1:1.
Bird fauna encountered at the main campus of the Islamic University of Gaza, Gaza City, Palestine
Authors: RABOU ABDEL FATTAH N. ABD
Published: March 2019

Abstract. Abd Rabou AN. 2019. Bird fauna encountered at the main campus of the Islamic University of Gaza, Gaza City, Palestine. Biodiversitas 20: 604-614. Birds are the most prominent and widespread wildlife species of both natural and urban environments worldwide. The strategic geographic position of Palestine at the meeting point of the three continents; Asia, Africa and Europe, is very crucial for the country's bird diversity. The Gaza Strip has never been studied for bird fauna in its urban environments. Thus, the current study comes to survey the bird fauna at the main campus (82,000 m2) of the Islamic University of Gaza (MC-IUG) as an urban environment. Birds were monitored using direct observations and binoculars for the lasted five years. Bird guidebooks and professional cameras were used throughout the succeeding stages of the study. A total number of 56 bird species, belonging to 26 families and 11 orders were encountered throughout the study at MC-IUG. Passeriformes was the largest order and comprised 35 (62.5%) of the recorded bird species, while the non-passerines constitute the remaining 21 species (37.5%). Muscicapidae was the largest among the recorded families, which was represented by 9 species (16.1%), followed by 8 species of Fringillidae (14.3%) and 5 species of Laniidae (8.9%). Finally, the greening strategy of the Gaza Strip should be adopted by Gazans and the local authorities due to the crucial role of vegetation cover in attracting more biodiversity components; especially bird fauna.
Evaluation of Salacca sumatrana as soil conservation crop in South Tapanuli, North Sumatra, Indonesia
Authors: NASUTION YUSRIANI
Published: March 2019

Abstract. Nasution Y, Rasyidin A, Yulnafatmawita, Saidi A. 2019. Evaluation of Salacca sumatrana as soil conservation crop in South Tapanuli, North Sumatra, Indonesia. Biodiversitas 20: 664-670. Salacca sumatrana Becc (snake fruit; or ‘Salak' in local name) is a profitable commodity. Morphologically, it is characterized by low, wide canopy that is potential intercept rainfall, while its roots are able to withstand soil erosion. This study is aimed to evaluate one variety of S. sumatrana named ‘salak sidimpuan" as commodity crop soil conservation based on its capability to intercept rain and trap soil sediment in the catchment area of S. sumatrana field. The research was conducted on mineral soils at West Angkola Sub-district, South Tapanuli District, North Sumatra Province, Indonesia with an altitude of 350-880 m above sea level. The study was carried out from October 2016 until March 2017 using survey method to calculate on rainfall interception, stemflow and throughfall while measurement of sediment used evaporation method. Statistical analysis was used to observe the difference of sediment trapped on the catchment area of S. sumatrana agroforest and the catchment area of mixed forest using Mann-Whitney Test. The results show that rainfall interception on S. sumatrana agroforest is 74.33% of the average rainfall while the Mann-Whitney Test Sig (2-tailed) is 0.000, indicating significant difference in trapping soil sediment between S. sumatrana agroforest and mixed forest. The amount of soil erosion on the catchment of S. sumatrana agroforest is 15.12 ton/ha/year while the erosion on mixed forest is 194.85 ton/ha/year. Thus S. sumatrana plant is capable to withstand rain interception with the result that soil erosion can be held in the catchment area of S. sumatrana agroforest. Instead of the calculation rain interception and soil erosion, then S sumatrana is classified as a soil conservation crop.
Short Communication: Molecular characteristic of taro white cattle based on DNA microsatellite markers
Authors: HERYANI LUH GEDE SRI SURYA , WANDIA I NENGAH , SUARNA I WAYAN , PUJA I KETUT , SUSARI NI NYOMAN WERDI , AGUSTINA KADEK KARANG
Published: March 2019

Abstract. Heryani LGSS, Wandia IN, Suarna IW, Puja IK, Susari NNW, Agustina KK. 2019. Short Communication: Molecular characteristic of taro white cattle based on DNA microsatellite markers. Biodiversitas 20: 671-675. This research was conducted to assess and characterize the genetics of Taro White cattle. Genetic characterizations of this cattle are essential to conservation and breeding program. A total of 18 samples and 4 pairs of microsatellite DNA markers (BM2113, BM1824, INRA023, and ETH225) were amplified by PCR and the products were run on 8% bis-Acrylamide gels. All microsatellite markers were successfully amplified with a mean allelic number of 3.25. Means of observed and expected heterozygosity were found to be 0,25 and 0,628. The Polymorphism Information Content (PIC) values ranged from 0.448 (BM1824) to 0.627 (BM2113) and fixation index were 0.620. The deviation from Hardy-Weinberg equilibrium revealed that Taro White cattle population exhibited significant deviations from Hardy-Weinberg equilibrium (HWE) and possessed a possibility of inbreeding. The microsatellite loci used or focused in the present study further validate their use for evaluation of genetic diversity of Taro White
Short Communication: Activity budget and diet in silvery lutung Trachypithecus cristatus at Gunung Padang, West Sumatra, Indonesia
Authors: AKBAR MUHAMMAD AZHARI , RIZALDI RIZALDI , NOVARINO WILSON , PERWITASARI-FARAJALLAH DYAH , TSUJI YAMATO
Published: March 2019

Abstract. Akbar MA, Rizaldi, Novarino W, Perwitasari-Farajallah D, Tsuji Y. 2019. Activity budget and diet in silvery lutung Trachypithecus cristatus at Gunung Padang, West Sumatra, Indonesia. Biodiversitas 20: 719-724. We studied the activity budget and diet of a group of wild silvery lutungs (Trachypithecus cristatus) in Gunung Padang, West Sumatra, Indonesia, with special attention to age-and sex-related differences. We conducted behavioral observations between July and October 2016, and found that resting constituted the greatest proportion of their daily activities (46%), followed by moving (38%), feeding (12%), and grooming (4%). Resting peaked between 11 am and 1 pm, while moving decreased in this period. The juveniles showed higher percentage of moving and lower percentage of feeding than the adults. The adult males showed higher percentage of grooming than the adult females. Finally, the nursing females showed lower percentage of resting and higher percentage of grooming than single females. During the study, we recorded 14 plant species consumed by the lutungs. Their dietary composition was composed of 63% foliage and 37% fruit. Both the foliage and fruits of Ficus variegata, a plant species belonging to the family Moraceae, was the most consumed. Foliage was frequently consumed by the nursing females and juveniles. The adult males were frequently observed to eat fruit during the study period. No fruit was consumed by the nursing females.
Detection and identification of Begomovirus infecting Cucurbitaceae and Solanaceae in Yogyakarta, Indonesia
Authors: SUBIASTUTI APRILIA SUFI , HARTONO SEDYO , DARYONO BUDI SETIADI
Published: March 2019

Abstract. Subiastuti AS, Hartono S, Daryono BS. 2019. Detection and Identification of Begomovirus infecting Cucurbitaceae and Solanaceae in Yogyakarta, Indonesia. Biodiversitas 20: 738-744. Begomovirus genome has high plasticity that led to evolve rapidly. Begomovirus is one of a remarkably successful group of emerging viruses as the results from combination of many factors. Planting systems in Indonesia which often overlapping two or more plant species in one land has high possibility for occurring mixed infection. It is also suggested has high contribution to increase Begomovirus diversity. The aim of this research is to do preliminary identification of Begomovirus infected-Solanaceae and Cucurbitaceae in Yogyakarta based core coat protein (CP) gene sequence. A total of 50 melon, 50 chili, 30 eggplants, and 30 watermelon samples which showed Begomovirus symptoms were observed from several fields in Yogyakarta and Purworejo, Indonesia during 2016. Almost 90% of infected samples for each plant were tested by PCR and showed positive for Begomovirus. Based on coat protein (CP) gene nucleotide sequence identity, Begomovirus infected Solanaceae in Indonesia has close relationship with Pepper yellow leaf curl Indonesia virus (PepYLCIV) and Tomato yellow leaf curl Kanchanaburi virus (TYLCVKaV), while in Cucurbitaceae has close relationship with Squash leaf curl China virus (SLCCV) Tomato leaf curl New Delhi virus (ToLCNDV). All collected isolates showed highest sequence identity with isolates from South-East Asia and China. However, further analysis that including full genome characterization is still needed to explain Begomovirus evolution in Indonesia.
The diversity of morphological characteristics and chemical content of Celosia cristata plantlets due to gamma ray irradiation
Authors: MUHALLILIN IZZATUL , AISYAH SYARIFAH IIS , SUKMA DEWI
Published: March 2019

Abstract. Muhallilin I, Aisyah SI, Sukma D. 2019. The diversity of morphological characteristics and chemical content of Celosia cristata plantlets due to gamma ray irradiation. Biodiversitas 20: 862-866. Celosia cristata L. or better known as Jengger Ayam is an ornamental plant that has a unique flower shape with attractive colors. Besides, this plant also contains antioxidants and chemical compounds that are used for traditional medicine to treat high blood pressure, and diabetes or as eye ailments. Increased diversity of C. cristata through hybridization techniques is constrained by flowers forming small grains that makes it difficult for castration and isolation of reproductive organs. This study aimed to increase C. cristata diversity of morphology and chemical content using mutation techniques induced by gamma irradiation. Plantlets were irradiated at 0, 25, 50 and 75 Gy doses. Scoring was conducted for 5 weeks until MV6 generation. Planlets were acclimatized for 3 weeks. The surviving plants were moved onto experimental land until seeded and tested for phytochemicals. Gamma irradiation with 25, 50 and 75 Gy doses produced 4 clones that have morphological changes in color and shape of the stem, leaves, and flowers. Changes in chemical content were observed with the appearance of triterpenoid compounds in 1 clone irradiated with 25 Gy.
A mutant variant of Nix gene on γ-irradiated Aedes aegypti as a male-determination factor
Authors: ERNAWAN BENI , SURNIYANTORO HARRY NUGROHO EKO , SUGORO IRAWAN , TAMBUNAN USMAN SUMO FRIEND
Published: March 2019

Abstract. Ernawan B, Surniyantoro HNE, Sugoro I, Tambunan USF. 2019. A mutant variant of Nix gene on γ-irradiated Aedes aegypti as a male-determination factor. Biodiversitas 20: 893-899. This study was aimed to examine the effect of γ-irradiation on the Nix gene sequence as a male-determination factor on Ae. aegypti. The novelty of the present study is the investigation on the gene mutation level of the deoxyribonucleic acid as an impact of ionizing radiation exposure. Males Ae. aegypti at the pupal stage were sterilized by applying 70 Gy γ-rays in varies dose-rates, i.e., 300, 600, 900, 1200 and 1500 Gy/h utilizing panoramic irradiator and males group without γ-irradiation as controls. Adult males that emerged from the pupal stage were assessed for the Nix gene sequence using a Real-Time Polymerase Chain Reaction method and were sequenced using Clustal Omega and Bioedit software, respectively. The results indicated that mutation occurred in Nix gene sequences were categorized as a point mutation. The change of Nix gene sequences was occurred between the nitrogenous base of guanine to adenine (G>A), thymine to adenine (T>A) and cytosine to adenine (C>A) in certain sequence numbers. There were six and nineteen on the forward-strand and reverse-strand of the Nix gene, respectively, due to γ-irradiation treatment. We conclude that γ-irradiation exposure causes point mutation on the Nix gene of males Ae. aegypti.
Morphological variation and heritability studies of Lagenaria siceraria landraces from northern KwaZulu-Natal, South Africa
Authors: BUTHELEZI LUNGELO GIVEN , MAVENGAHAMA SYDNEY , NTULI NONTUTHUKO ROSEMARY
Published: March 2019

Abstract: Lagenaria siceraria (Molina) Standley belongs to the Cucurbitaceae family. Its nutritious tender shoots, fruits, and seeds are widely consumed in rural communities as leafy vegetables. However, studies on variation and heritability of its agronomic traits are lacking. This study aimed to characterise variability and heritability among L. siceraria genotypes with various fruit and seed morphology from different origins. Seedling, vegetative and reproductive traits were compared among landraces. Landraces varied significantly in their seedling, vegetative and reproductive traits. Significant positive correlations were mainly recorded among reproductive traits. The first five informative principal components had a total variability of 74.393%. Biplot and dendrogram grouped landraces mainly according to fruit and seed morphology and then their origin. In a biplot, Cluster-I grouped landraces with pear-shaped (KSP, RSP, RRP) and curvilinear-shaped (NqRC) fruits. Cluster-II mainly associated landraces with smooth and curvilinear-shaped fruits (KSC, NqSC, NSRC, NSC, MSC and DSI) from different origins. Cluster- III associated landraces with rough textured fruits from the same origin (NRB and NRC). High heritability estimate was recorded among fruit and seed traits. This was the first comprehensive variability and heritability study in the country and therefore it formed basis for available L. siceraria germplasm essential for future breeding programs.
Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria
Authors: AYUNINGRUM DIAH , KRISTIANA RHESI , NISA AYUNDA AINUN , RADJASA SEPTHY KUSUMA , MUCHLISSIN SAKTI IMAM , RADJASA OCKY KARNA , SABDONO AGUS , TRIANTO AGUS
Published: March 2019

Abstract. Ayuningrum D, Kristiana R, Nisa AA, Radjasa SK, Muchlissin SI, Radjasa OK, Sabdono A, Trianto A. 2019. Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria. Biodiversitas 20: 956-964. Tunicate is a rich secondary metabolites producer with various biological activities whether as an original producer or produced by the associated microorganisms. In this study, a total of 11 tunicate specimens were identified as Polycarpa aurata with four color variations based on morphological characteristic and COI gene identification and BLAST analysis. The P. aurata associated-bacteria were isolated and tested for antimicrobial activity against multi-drug resistant (MDR) bacteria. A total of 86 axenic isolates were successfully purified. Furthermore, nine isolates (10.5%) exhibited antibacterial activity on preliminary screening. Nine prospective isolates were fermented in respective medium (Zobell 2216, modified M1 or modified ISP2 media) then extracted using ethyl acetate. The ethyl acetate extracts from liquid fermentation were tested against MDR Escherichia coli, MDR Bacillus cereus, Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin-Sensitive and Staphylococcus aureus (MSSA). As a result, seven isolates (8.1%) still retained the activity at the extract concentration 150 µg/disk. Molecular analysis based on 16S rDNA sequencing revealed the most active isolates, TSB 47, TSC 10 and TSB 34 identified as Bacillus tropicus, Vibrio alginolyticus and Virgibacillus massiliensis, with BLAST homology 99%.
Economic valuation of Lansa Mangrove Forest, North Sulawesi, Indonesia
Authors: SONDAK CALVYN FA , KALIGIS ERLY Y , BARA ROBERT A
Published: March 2019

Abstract. Sondak CFA, Kaligis EY, Bara RA. 2019. Economic valuation of Lansa Mangrove Forest, North Sulawesi, Indonesia. Biodiversitas 20: 978-986. Mangrove forest gives many benefits and services to human and environment. Even though it contributes many benefits and services, coastal ecosystems threatened as one of the most critical ecosystems in the world. The study aims to estimate the economic value of ecosystem services provided by Lansa mangrove forest, Wori Sub-district, North Minahasa District, North Sulawesi Province, Indonesia. Here, we describe the use value (direct and indirect value) and non-use value (option and existence value), and emphasize the components of ecosystem services fish resources, firewood, coastal protection, biodiversity, carbon (C) removal and mangrove sustainability because these directly influence human welfare. Their market price calculated fish and C removal value. Coastal barrier and firewood were approached using the replacement cost method. Biodiversity value was calculated using Indonesia mangrove forest biodiversity value. Contingent Valuation method was used to find out people willing to pay for the sustainability of mangrove forest. Lansa mangrove forest total economic value (TEV) was IDR 4,431,197,603 or equal to US$ 305,600 (US$ 1,959 ha-1). The success of this mangrove valuation has potentially large implications for future policy-making of its’ ecosystem service values.
Short Communication: Population and stand structure of Cinnamomum sintoc in the Low Land Forest of Mount Ciremai National Park, West Java, Indonesia
Authors: ISMAIL AGUS YADI , KUSMANA CECEP , SUDIANA EMING , WIDODO PUDJI
Published: March 2019

Abstract. Ismail AY, Kusmana C, Sudiana E, Widodo P. 2019. Short Communication: Population and stand structure of Cinnamomum sintoc in the Low Land Forest of Mount Ciremai National Park, West Java, Indonesia. Biodiversitas 20: 1042-1047. Cinnamomum sintoc (C. sintoc) is one of the anthelmintic and anti-inflammatory medicinal plants which produces essential oils from its roots, skin, and leaves. This study aimed to analyze the population and stand structure of C. sintoc in Mount Ciremai National Park, West Java, Indonesia. Data collection was carried out using plots/ strips method. The plots were purposively placed and made at each altitude and direction of the slope. The research recorded 804 individual trees from each growth edge. Individual density between slope directions and altitudes was not significantly different for all diameter classes, but was significantly different between slope directions of stratum B (P = 0.001) and E (P = 0.012). These results indicate that sintoc population is spread evenly and can be cultivated at various slope and altitude directions and has no preference for the two topographic variables.
Orchids Diversity in the Sicikeh-Cikeh Forest, North Sumatra, Indonesia
Authors: HARTINI SRI
Published: March 2019

Abstract. Hartini S. 2019. Orchids Diversity in the Sicikeh-Cikeh Forest, North Sumatra, Indonesia. Biodiversitas 20: 1087-1096. Sicikeh-cikeh forest includes three forest areas, namely Adian Tinjoan Customary Forest, Adian Tinjoan Protection Forest, and Taman Wisata Alam Sicikeh-cikeh. Typical vegetation of this area is mountain forest with large diversity of plant species. Among the species, orchid is one of the potential plants found diversely in this location and has not yet been recorded. The exploration activities were conducted at Sicikeh-cikeh forest. Orchid exploration was conducted to collect living plants for ex situ conservation purpose. Orchid inventory, to record orchid diversity in this area, was based on plant collection by purposive random sampling. The results of the study recorded approximately 102 different species from 30 genera of orchids in this area. Typical epiphytic and terrestrial highland orchids were found and very common in Sumatra. Among the species found are endemic Sumatra, such as Coelogyne brachygyne, Coelogyne salmonicolor, Dendrobium kruiense, Epigeneium pulchellum, Thrixspermum gombakense, Corybas stenotribonos and Paphiopedilum tonsum. Interesting terrestrial orchids include Kuhlhasseltia javanica, Neuwiedia zollingeri var. javanica, Paphiopedilum tonsum, Phaius callosus, Corybas stenotribonos, Calanthe aurantiaca, Calanthe chrysoglossoides, and Calanthe pulchra. While interesting epiphytic orchids include Bulbophyllum absconditum, Bulbophyllum coloriferum, Coelogyne brachygyne, Epigenium pulchellum, Eria bractescens, Eria pachystachya, Pholidota gibbosa, and Thrixspermum amplexicaule.
Short Communication: Correlation and regression among rice panicle branches traits
Authors: HASTINI TRI , SUWARNO WILLY BAYUARDI , GHULAMAHDI MUNIF , ASWIDINNOOR HAJRIAL
Published: March 2019

Abstract. Hastini T, Suwarno WB, Ghulamahdi M, Aswidinnoor H. 2019. Short Communication: Correlation and regression among rice panicle branches traits. Biodiversitas 20: 1140-1146. Panicle was a very important organ in rice since the grains are developed from the panicle branches. This study aimed to find out the information about the relationship among rice panicle branching traits using correlation and regression analysis. The trial was conducted at the IPB Research Station, Babakan, Bogor, Indonesia from December 2015 until April 2016. Twenty genotypes of lowland rice were used as treatments with three replications and arranged in a completely randomized design. The panicle branches traits were observed. The result showed that there was positively and a highly significant relationship among the panicle branches traits in general. Analysis using linear regression revealed that, in general, the total grains number per panicle showed the linear relationships with other traits, and only total grains number per panicle vs tertiary branches number, total grains number per panicle vs grains number of tertiary panicle branch, and total grains number per panicle vs the number of secondary branches per panicle branch showed the quadratic relationship. Among all rice panicle branching traits, the secondary panicle branches number had the highest contribution to the total grains number per panicle.
Short Communication: Genetic diversity of scalloped hammerhead sharks (Sphyrna lewini) landed in Muncar Fishing Port, Banyuwangi
Authors: ALGHOZALI FAQIH AKBAR , WIJAYANTI DIAH PERMATA , SABDONO AGUS
Published: March 2019

Abstract. Alghozali FA, Wijayanti DP, Sabdono A. 2019. Short Communication: Genetic diversity of scalloped hammerhead sharks (Sphyrna lewini) landed in Muncar Fishing Port, Banyuwangi. Biodiversitas 20: 1154-1159. The majority of sharks caught in Indonesian fisheries were bycatch products from the tuna longline fisheries, but some regions in Indonesia fish the sharks as their main target. One of these regions is located in Muncar, Banyuwangi, which fishes the endangered Scalloped Hammerhead sharks (Sphyrna lewini) as target species. This research aimed to study the genetic diversity of the endangered Scalloped Hammerhead sharks landed in Muncar Fishing Port, Banyuwangi. Genetic analysis was done through PCR (Polymerase Chain Reaction) amplification and sequencing of the mitochondrial DNA COI (Cytochrome Oxidase subunit I) gene. Out of the 37 samples collected, 30 were successfully amplified and sequenced.The results showed moderate haplotype diversity (Hd: 0,582 ± 0,079) and low nucleotide diversity (π: 0,00392± 0,0024) with five haplotypes (h) and 26 polymorphic sites (S). Tajima’s D neutrality model values indicated a population expansion event. Two different clades were determined through phylogenetic analysis and by GenBank sequences comparison. These results provided basic information and present status of the Scalloped Hammerhead sharks population genetically within the fishing ground (Makassar Strait-Kangean Islands).
Extended low temperature and cryostorage longevity of Salix seeds with desiccation control
Authors: Ku Ja Jung , Han Sim Hee , Kim Du Hyun
Published: March 2019

Salix xerophila, S. maximowiczii, and S. koreensis are species of willow native to Korea that are important for bioenergy production. However, the native range of these species has narrowed in recent years due to the impact of climate change. Seeds of these Salix species lose viability within 4 weeks at ambient temperature, and within 4 months at -4°C. Preservation techniques are urgently needed to protect these valuable resources. The effects of seed water content (SWC; 3%, 6%, 9%, 12%, 18%, and 24%) and temperature (ambient, 4°C, -18°C, -80°C, and -196°C) on storage stability were investigated for up to 48, 52, or 60 months, depending on species. Optimal storage temperature and SWC varied between species. S. xerophila seed could be stored without deterioration for 60 months with 9% SWC at -80°C, but rapidly lost viability when stored at -18°C. In S. maximowiczii and S. koreensis, 100% and 90% of normal germination, respectively, was maintained with 18% SWC at -18°C or -80°C. Thus, for some Salix species, storage at -18 and -80°C may provide an economical alternative to cryopreservation or medium-term storage for the maintenance of seedbanks or breeding stocks.
The number of regulatory B cells is increased in mice with collagen-induced arthritis
Authors: Luo Li , Liu Qing , Peng Shanshan , Meng Yan , Du Wenjing , Luo Demei , Wang Qian , Ding Jianbing , Dong Xunan , Ma Xiumin
Published: March 2019

The aim of this study is to investigate changes in regulatory B cells (Bregs) and the expression of related cytokines such as interleukin-10 (IL-10) and transforming growth factor (TGF)-β in a mouse model of collagen-induced arthritis (CIA). A total 20 DBA/1 mice (6-8 weeks old) were randomly divided into control and CIA disease groups. For the CIA disease group, animals were injected intradermally with chicken collagen type II and complete Freund's adjuvant. The calculated arthritis index score of the CIA group was significantly higher than that in control group. Hematoxylin and eosin staining showed tumid synovial cells with irregular arrangement and obvious hyperplasia, with a high degree of inflammatory cell infiltration in CIA model group. Cytometric bead array technology and quantitative RT-PCR indicated that the levels of IL-10 and TGF-β in serum, and synovial cells were significantly increased in the CIA group. The proportion of Bregs in the spleen of the CIA group was significantly increased compared to the control group. In conclusion, our findings demonstrate that the number of Bregs and the expression of TGF-β and IL-10 are enhanced in mice with CIA.
Genome-wide analysis of the WRKY gene family and its response to abiotic stress in buckwheat (Fagopyrum tataricum)
Authors: He Xia , Li Jing-jian , Chen Yuan , Yang Jia-qi , Chen Xiao-yang
Published: March 2019

The WRKY gene family is an ancient plant transcription factor (TF) family with a vital role in plant growth and development, especially in response to biotic and abiotic stresses. Although many researchers have studied WRKY TFs in numerous plant species, little is known of them in Tartary buckwheat (Fagopyrum tataricum). Based on the recently reported genome sequence of Tartary buckwheat, we identified 78 FtWRKY proteins that could be classified into three major groups. All 77 WRKY genes were distributed unevenly across all eight chromosomes. Exon–intron analysis and motif composition prediction revealed the complexity and diversity of FtWRKYs, indicating that WRKY TFs may be of significance in plant growth regulation and stress response. Two separate pairs of tandem duplication genes were found, but no segmental duplications were identified. Overall, most orthologous gene-pairs between Tartary and common buckwheat evolved under strong purifying selection. qRT-PCR was used to analyze differences in expression among four FtWRKYs (FtWRKY6, 74, 31, and 7) under salt, drought, cold, and heat treatments. The results revealed that all four proteins are related to abiotic stress responses, although they exhibited various expression patterns. In particular, the relative expression levels of FtWRKY6, 74, and 31 were significantly upregulated under salt stress, while the highest expression of FtWRKY7 was observed from heat treatment. This study provides comprehensive insights into the WRKY gene family in Tartary buckwheat, and can support the screening of additional candidate genes for further functional characterization of WRKYs under various stresses.
Stabilizability of Infinite Dimensional Systems by Finite Dimensional Control
Authors: Raymond Jean-Pierre
Published: March 2019

In this paper, we consider control systems for which the underlying semigroup is analytic and the resolvent of its generator is compact. In that case we give a characterization of the stabilizability of such control systems. When the stabilizability condition is satisfied, the system is also stabilizable by finite dimensional controls.
We end the paper by giving an application of this result to the stabilizability of the Oseen equations with mixed boundary conditions.
Modulo p parabolic induction of pro-p-Iwahori Hecke algebra
Authors: Abe Noriyuki
Published: March 2019

We study the structure of parabolic inductions of a pro-p-Iwahori Hecke algebra.
In particular, we give a classification of irreducible modulo p representations of pro-p-Iwahori Hecke algebras in terms of supersingular representations.
Since supersingular representations are classified by Ollivier and Vignéras, it completes the classification of irreducible modulo p representations.
A note on the formulas for the Drazin inverse of the sum of two matrices
Authors: Liu Xin , Yang Xiaoying , Wang Yaqiang
Published: March 2019

In this paper we derive the formula of (P + Q)D under the conditions Q(P + Q)P(P + Q) = 0, P(P + Q)P(P + Q) = 0 and QPQ2 = 0. Then, a corollary is given which satisfies the conditions (P + Q)P(P + Q) = 0 and QPQ2 = 0. Meanwhile, we show that the additive formula provided by Bu et al. (J. Appl. Math. Comput. 38 (2012) 631-640) is not valid for all matrices which satisfies the conditions (P + Q)P(P + Q) = 0 and QPQ2 = 0. Also, the representation can be simplified from Višnjić (Filomat 30 (2016) 125-130) which satisfies given conditions. Furthermore, we apply our result to establish a new representation for the Drazin inverse of a complex block matrix having generalized Schur complement equal to zero under some conditions. Finally, a numerical example is given to illustrate our result.
Perbandingan Kualitas Semen Beku Sapi Unggul dan Hubungannya dengan Tingkat Keberhasilan Inseminasi Buatan pada Sapi Aceh
Authors: Fatah Khairul , Dasrul Dasrul , Abdullah Mohd. Agus Nashri
Published: March 2019

ABSTRAK. Penelitian ini merupakan penelitian observasional yang dengan  rancangan acak lengkap (RAL) dengan 3 kelompok perlakuan jenis semen beku sapi aceh (S1), semen beku sapi bali (S2) dan semen beku sapi simmental (S3).  Masing-masing kelompok diulang sebanyak 10 kali. Data kualitas spermatozoa dan angka kebuntingan yang diperoleh dianalisis dengan analisis of variance (ANOVA) dan dilanjutkan dengan uji Duncan dan hubungan kualitas spermatozoa semen beku dengan tingkat kebuntingan diuji dengan regresi berganda.  Persentase motilitas, spermatozoas hidup dan TAU sapi aceh dan sapi bali tidak memperlihatkan perbedaan yang nyata (P0,05), namun keduanya berbeda secara nyata (P0,05) dengan sapi simmental. Hasil uji regresi menunjukan ada hubungan yang nyata (P0,05) antara kualitas spermatozoa (motilitas, spermatozoa hidup dan TAU) semen beku dengan tingkat kebuntingan, dengan persamaan regresi adalah  Y=  - 2,586 + 0,017 X1 - 0,001 X2 + 0,043 X3, nilai koefisien korelasi r = 0,695 dan nilai koefisien determinasi (r2) sebesar 0,483. Keutuhan TAU memiliki hubungan yang lebih kuat (r = 0,695) dibanding dengan motilitas spermatozoa (r = 0,505) dan spermatozoa hidup (r = 0,195) terhadap angka kebuntingan. Disimpulkan kualitas semen beku sapi unggul berpengaruh terhadap tingkat kebuntingan setelah inseminasi pada induk aseptor sapi aceh betina.(Comparison of frozen semen quality of aceh cattle,  bali cattle,  brahman cattle and simmental cattle and the relationship with the success level of artificial insemination in female aceh cattle)ABSTRACT. This study is an observational study with a completely randomized design (CRD) with 3 groups of frozen semen aceh cattle (S1), bali cattle frozen semen (S2) and simmental cattle frozen semen (S3). Each treatments groups was repeated 10 times. Spermatozoa quality data (motility, live spermatozoa, and intact acrosome) of frozen semen and pregnancy rates obtained were analyzed by analysis of variance (ANOVA) and continued with Duncant test. Percentage motility, live sperm and intact acrosome of aceh cattle, bali cattle and simmental cattle showed no significant differences (P 0.05), but they differ significantly (P 0.05) compared with brahman cattle. Results of regression analysis showed no significant relationship (P 0.05) between spermatozoa quality (motility, live sperm and intact acrosome) with a pregnancy rate of frozen semen, the regression equation is Y = - 2,586 + 0,017 X1 - 0,001 X2 + 0.043 X3, Correlation coefficient value r = 0,695 and coefficient of determination (r2) equal to 0,483. intact acrosome of spermatozoa have a stronger relationship (r = 0.695) compared with sperm motility (r = 0.505) and live spermatozoa (r = 0.195) on the pregnancy rate. It was concluded that the quality of frozen semen had an effect on the pregnancy rate after insemination on female aceh cow.
Pengembangan Peternakan Sapi Potong melalui Program Klaster: Deskripsi Program dan Kegiatan
Authors: Sodiq Akhmad , Yuwono Pembudi , Wakhidati Yusmi Nur , Sidhi Arif Harnowo , Rayhan Muhammad , Maulianto Arief
Published: March 2019

ABSTRAK. Tujuan penelitian ini adalah mendeskripsikan program dan kegiatan pengembangan klaster sapi potong. Peternakan rakyat sapi potong yang tergabung dalam kelompok tani ternak dan masuk kategori Usaha Mikro Kecil Menengah (UMKM) dilibatkan pada penelitian ini. Variabel-variabel yang terkait dengan program dan kegiatan penguatan UMKM Sapi Potong dikoleksi. Data dianalisis menggunakan analisis deskriptif dan Root Cause Analysis. Program dan kegiatan pengembangan klaster sapi potong mencakup (i) program penguatan kelembagaan kelompok, (ii) program penguatan teknologi peternakan dan (iii) program penguatan akses pembiayaan perbankan. Program penguatan kelembagaan kelompok melalui kegiatan pendidikan dan pelatihan, peningkatan kerjasama (untuk penyediaan pakan dan modal, penanggulangan penyakit, pemasaran), peningkatan pertemuan antar anggota kelompok dan lembaga lain, peningkatan penguasaan teknologi diversifikasi. Program penguatan teknologi peternakan dengan kegiatan pelatihan berbagai teknologi peternakan, studi banding dan magang, pendampingan penerapan proven teknologi berorientasi peningkatan fisibilitas usaha. Untuk program penguatan akses pembiayaan perbankan berupa kegiatan sosialisasi pembiayaan perbankan dan pendampingan akses pembiayaan perbankan. (Beef cattle development through beef cattle cluster program: description of programs and activities) ABSTRACT. The purpose of this study was to describe the design of programs and activities of beef cattle clusters. Beef cattle farmer group (Micro Small Medium Enterprises, MSME) were involved in this study. Variables related to program and activities for empowering MSME were collected. Descriptive and Root Cause Analysis were applied in this study. The design of program and activities for beef cattle cluster include (i) program of empowering farmer groups, (ii) programs for strengthening livestock technology, and (iii) programs for increasing accessibility getting financial support from the bank. Program of empowering farmer groups involved education and training, increasing cooperation (for feeding capital, disease control, marketing), increasing cooperation among farmer group members and other institutions, transfer technology. Programs for strengthening livestock technology by activities such as training of livestock technologies, internship, provide proven technology for improving feasibility. The socialization of financial scheme, and assistance activities were involved for accelerating financial support from the bank.
Pengaruh Pemberian Ampas Kedelai dan Bungkil Inti Sawit (AKBIS) yang Difermentasi dengan Aspergillus niger terhadap Kadar Lemak dan Kolesterol Daging Dada Broiler
Authors: Syafrizal Syafrizal , Nurliana Nurliana , Sugito Sugito
Published: March 2019

ABSTRAK. Penelitian ini bertujuan mengetahui efek penurunan kadar lemak dan kolesterol pada broiler yang diberikan AKBIS yang difermentasi dengan A. niger. Dalam penelitian ini digunakan 80 ekor broiler. Sampel daging bagian dada diperoleh dari 20 ekor broiler yang diambil secara acak. Sampel diperiksa kadar lemak dengan uji proksimat dan pemeriksaan kolesterol dengan uji Liebermann Burchard. Data yang diperoleh dianalisis dengan uji ANOVA dilanjutkan dengan uji regresi. Hasil penelitian menunjukkan bahwa pakan pemberian AKBIS fermentasi mampu menurunkan kadar lemak dan kolesterol daging dada broiler secara signifikan (P0,05). Penurunan kadar lemak dan kolesterol sangat dipengaruhi oleh serat kasar dan kandungan enzim yang terdapat dalam pakan fermentasi. Dari penelitian ini dapat disimpulkan pemberian AKBIS fermentasi sangat efektif untuk menurunkan kadar lemak dan kolesterol pada daging broiler sehingga aman dikonsumsi serta tidak membahayakan kesehatan.  (The administration effect of soybean dregs and palm kernel meal fermented with Aspergillus niger on fat and cholesterol levels of broiler meat) ABSTRACT. This study aims to determine the effect of decreasing levels of fat and cholesterol in broiler given fermented feed with A. niger. This study used 80 broilers. Chest meat samples obtained from randomly 20 broilers. Samples were examined by proximate test and cholesterol with Liebermann Burchard method. Data were analyzed by ANOVA followed by regression. The results showed that fermented feed could significantly decrease fat and cholesterol level in broiler breast meat (P 0.05). The decreased levels of fat and cholesterol is strongly influenced by the content of crude fiber and enzymes found in fermented feed. This study concluded that the administration of fermented feed able to reduce levels of fat and cholesterol in broiler meat and it is safe for consumption and not harmful to health.
Some Aspects of Vascular Theory of Development and Progression of Primary Open-Angle Glaucoma (Literature Review). Part 1
Authors: Makogon S. I. , Makogon A. S.
Published: March 2019

The article presents a review of studies on various aspects of vascular theory of primary open-angle glaucoma (POAG) development and progression. The data of foreign and domestic scientists on the influence of hemodynamic disturbances in the glaucoma pathogenesis, including various levels: Central, regional and microcirculatory. Violations of the universal mechanism of vascular tone regulation, namely endothelial dysfunction are described. Vascular endothelium is a barrier between vascular wall and blood plasma. When the endothelium is damaged, its antithrombotic properties are transformed into a powerful prothrombotic potential. This leads to an imbalance between vasoconstrictors (endothelin) and vasodilators (nitric oxide). In addition, the release of biologically active substances with local neurotoxic effects. The authors noted an increase in the concentration of endothelin-1, studied its interaction with nitrogen oxide and considered its role in the occurrence of ischemia and the impact on the death of retinal ganglion cells. Scientists have noticed an increase in the level of Willebrand factor and increased platelet aggregation in patients with progressive glaucoma. As a possible cause of hemodynamic disturbances and glaucoma progression, the authors consider changes in blood rheological properties. In addition, the state of the three closely interacting components: the wall of blood vessels (primarily endothelium and subendothelial structures); cellular elements of blood (primarily platelets) and plasma proteolytic systems ensure the normal functioning of the hemostatic system. Changes in of these components state may affect the hemostatic system. Therefore, further study of various directions of vascular theory will help in understanding the etiopathogenesis of glaucoma.
Stimulation of Basal Secretion in the Treatment of Dry Eye Syndrome
Authors: Uchakova L. I. , Balalin S. V. , Zagrebin V. L.
Published: March 2019

The aim is to evaluate the effectiveness of Stillavit in the treatment of dry eye syndrome (DES) and its effect on the density of conjunctival goblet cells and the functional parameters of tear production. Methods. There were analyzed treatment results of 18 patients (36 eyes) with DES at the age of 48 to 80 years (average 60.6 ± 1.3 years) including 17 women and 1 male before and after 1 and 3 months after Stillavit prescription. In all patients in addition to a standard ophthalmological examination the results of Schirmer I, Norn, as well as the LIPCOF test were evaluated and the density of conjunctival goblet cells was examined. Results. After 1 and 3 months there was a significant improvement in the results of the Norn test: from 5.17 ± 0.22 seconds to 7.18 ± 0.17 seconds which indicated an increase in the stability of the tear film. After 3 months there was a significant increase in the density of conjunctival goblet cells, improvement in the Schirmer sample: from 9.26 ± 0.89 to 11.7 ± 0.6 mm (t = 2.28, p < 0.05) and LIPCOF test (t = 2.18, p < 0.05) which indicated an increase in tear production and a significant decrease in the inflammatory process and edema of the conjunctiva. Conclusion. Treatment of DES with the use of Stillavit leads after 3 months to a significant increase in the density of conjunctival goblet cells, increases tear production and stability of the tear film.
Perfect Prismatoids and the Conjecture Concerning Face Numbers of Centrally Symmetric Polytopes
Authors: Kozachok M. A.
Published: March 2019

In this paper we introduce and study a class of centrally symmetric polytopes – perfect prismatoids – and some its properties related to the famous conjecture concerning face numbers of centrally symmetric polytopes are proved. It is proved that any Hanner polytope is a perfect prismatoid and any perfect prismatoid is affine equivalent to some 0/1-polytope.
Verification of Computing Grids with Special Edge Conditions by Infinite Petri Nets
Authors: Zaitsev D. A.
Published: March 2019

A technique of the computing grid verification using invariants of infinite Petri nets was presented. Models of square grid structures in the form of parametric Petri nets for such edge conditions as connection of edges and truncated devices were constructed. Infinite systems of linear algebraic equations were composed on parametric Petri nets for calculating p-invariants; their parametric solutions were obtained. P-invariant Petri nets are structuraly conservative and bounded that together with liveness are the properties of ideal systems. Liveness investigation based on siphons and traps can be implemented by using p-invariants of modified nets.
Deductive Verification of Telecommunication Systems Written in C
Authors: Anureev I. S.
Published: March 2019

A deductive approach to verification of telecommunication systems written in C is proposed. The approach is based on the extension of C by declarative statements and on reduction of verification of parallel communicating components of these systems to separate verification of components written in this extension. An example of verification of a data link protocol is considered.
An Approach to Concretization of Test Scenarios in Industrial Automation Testing of Software Projects
Authors: Kolchin A. V. , Letichevsky A. A. , Peschanenko V. S. , Drobintsev P. D. , Kotlyarov V. P.
Published: March 2019

In this paper we propose an approach to efficient automating test technology for industrial software projects, that uses a formal model of the system, automatically performs a symbolic verification, generation and concretization of the symbolic traces, the generation of test suites for concretized traces, and also includes tools for analysis of the testing results, allowing users to automate the full cycle of testing. Particular emphasis is placed on the presentation of the algorithm concretization and setting of test scenarios.
Unbiased Entropy Estimator for Binary Sequences
Authors: Timofeev E. A.
Published: March 2019

A new class of metrics on a space of right-sided infinite sequences drawn from a binary alphabet was introduced.
The Effect of Domain Deformation on the Behavior of a Distributed Kinetic System
Authors: Kubyshkin E. P.
Published: March 2019

The behavior of a distributed kinetic system, which is in homogeneous equilibrium within a flat circular reactor, under circular domain deformation is studied. We show that the deformation of domain may lead to appearance of stable spatially inhomogeneous oscillatory solutions, including chaotic oscillations (strange attractors), in the neighborhood of homogeneous equilibrium. We also speak about mechanisms of initiation of chaotic attractors and calculate Lyapunov exponents and Lyapunov dimension for these regimes. We call this mechanism of appearance of spatially inhomogeneous nonlinear oscillations in distributed kinetic system the domain effect.
On the Efficient Representation of an Unbounded Resource with the Aid of One-Counter Circuits
Authors: Bashkin V. A.
Published: March 2019

A class of infinite-state automata with a simple periodic behaviour and a convenient graphical representation is studied. A positive one-counter circuit is defined as a strongly connected one-counter net (one-counter nondeterministic finite automata without zero-testing) with at least one positive cycle. It is shown that in a positive circuit an infinite part of a reachability set is an arithmetic progression; numerical properties of this progression are estimated. A specific graphical representation of circuits is presented. General one-counter nets are equivalent to Petri Nets with at most one unbounded place and to pushdown automata with a single-symbol stack alphabet. It is shown that an arbitrary one-counter net can be represented by a finite tree of circuits. A one-counter net is called sound, if a counter is used only for “infinite-state” (periodic) behaviour. It is shown that for an arbitrary one-counter net an equivalent sound net can be effectively constructed from the corresponding tree of circuits.