bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy of the first bioactive peptide from the pearl
Authors: Wu Chaoyi , Yin Zehui , Wang Yayu , Chen Xinjiani , Li Bailei , Wang Qin , Yao Liping , Zhang Zhen , Liu Xiaojun , Zhang Rongqing
Published: October 2023

Pearls have high medicinal value. In the present study, we discovered the first bioactive peptide in pearls. The bioactive peptide, KKCHFWPFPW, was a novel angiotensin I-converting enzyme (ACE)-inhibitory peptide derived from the pearl matrix ofPinctada fucata. It was screened and identified using quadrupole time-of-flight mass spectrometry. The molecular weight of the peptide was 1417.5 Da, and its theoretical isoelectric point was 9.31. The half-maximal inhibitory concentration of the peptide was 4.17μM, as determined by high-performance liquid chromatography. The Lineweaver–Burk plot showed that this peptide competitively inhibited ACE activity. As the peptide concentration increased, the ACE inhibition rate also increased. The molecular docking was simulated using Maestro 2022-1 Glide software to understand the potential mechanisms underlying the ACE-inhibitory activity of KKCHFWPFPW. These results indicated that the peptide from theP. martensiipearl matrix might be a potential source of antihypertensive peptides.
Complex genomic landscape of inversion polymorphism in Europe’s most destructive forest pest
Authors: Mykhailenko Anastasiia , Zieliński Piotr , Bednarz Aleksandra , Schlyter Fredrik , Andersson Martin N. , Antunes Bernardo , Borowski Zbigniew , Krokene Paal , Melin Markus , Morales-García Julia , Müller Jörg , Nowak Zuzanna , Schebeck Martin , Stauffer Christian , Viiri Heli , Zaborowska Julia , Babik Wiesław , Nadachowska-Brzyska Krystyna
Published: October 2023

In many species, polymorphic inversions underlie complex phenotypic polymorphisms and facilitate local adaptation in the face of gene flow. Multiple polymorphic inversions can co-occur in a genome, but the prevalence, evolutionary significance, and limits to complexity of genomic inversion landscapes remain poorly understood. Here, we examine genome-wide variation in one of Europe’s most destructive forest pests, the spruce bark beetleIps typographus, scan for polymorphic inversions, and test whether inversions are involved in key adaptations in this species. We analyzed 240 individuals from 18 populations across the species’ European range and, using a whole-genome resequencing approach, identified 27 polymorphic inversions covering approximately 28% of the genome. The inversions vary in size and in levels of intra-inversion recombination, are highly polymorphic across the species range, and often overlap, forming a complex genomic architecture. We test several mechanisms, including directional selection, overdominance and associative overdominance that can contribute to the maintenance of inversion polymorphisms in the genome. We show that the heterogeneous inversion landscape is likely maintained by the combined action of several evolutionary forces and that inversions are enriched in odorant receptor genes encoding key elements of recognition pathways for host plants, mates, and symbiotic fungi. Our results indicate that the genome of this major forest pest of growing social, political, and economic importance harbors one of the most complex inversion landscapes described to date posing a question about limits of genomic architecture complexity.
The maintenance of genetic polymorphism underlying sexually antagonistic traits
Authors: Flintham Ewan , Savolainen Vincent , Otto Sarah , Reuter Max , Mullon Charles
Published: October 2023

Abstract

Selection often favours different trait values in males and females, leading to genetic conflicts between the sexes when traits have a shared genetic basis. Such sexual antagonism has been proposed to maintain genetic polymorphism. However, this notion is based on insights from population genetic models of single loci with fixed fitness effects. It is thus unclear how readily polymorphism emerges from sex-specific selection acting on continuous traits, where fitness effects arise from the genotype-phenotype map and the fitness landscape. Here we model the evolution of a continuous trait that has a shared genetic basis but different optima in males and females, considering a wide variety of genetic architectures and fitness landscapes. For autosomal loci, the long-term maintenance of polymorphism requires strong conflict between males and females that generates uncharacteristic sex-specific fitness patterns. Instead, more plausible sex-specific fitness landscapes typically generate stabilising selection leading to an evolutionarily stable state that consists of a single homozygous genotype. Except for sites tightly linked to the sex determining region, our results indicate that genetic variation due to sexual antagonism should arise only rarely and often be transient, making these signatures challenging to detect in genomic data.
Experimental and methodological framework for the assessment of nucleic acids in airborne microorganisms
Authors: Péguilhan Raphaëlle , Rossi Florent , Rué Olivier , Joly Muriel , Amato Pierre
Published: October 2023

Studying airborne microorganisms is highly challenging due to ultra-low and spread biomass, and great spatial and temporal variabilities at short scales. Aeromicrobiology is still an emerging discipline of environmental microbiology, and some of the basic practices (replication, control of contaminants,etc) are not yet widely adopted, which potentially limits conclusions. Here we aim at evaluating the benefits of such practices in the study of the aeromicrobiome using molecular-based approaches, and recommend the following: (i) sample at high airflow rate, if possible into a fixative agent, in order to be able to capture specific situations ; (ii) replicate sampling and process samples individually to enable statistical analyses ; (iii) check for contaminants at different steps of the analytical process, and account for their potential stochasticity in sequence decontamination methods ; (iv) include internal references to verify qualitative and quantitative aspects of the data, and (v) eventually investigate multiple analytical procedures to identify potential impacts on the data. In our study, samples were collected at a remote mountain site using high-flow rate impingers collecting airborne material into nucleic acid preservation buffer. As high of ∼75% of the sequences were shared between independent triplicates, gathering 28 to 38% of the richness observed at the ASV level at a given sampling date, which also emphasizes spatial heterogeneity at short scale due to rare taxa. Thanks to replicates, daily variations in the diversity of bacteria could be distinguished statistically, and the inevitable presence of contaminating sequences in controls could be accounted for using established statistical methods. This work opens new perspectives and notably paves the way to untargeted molecular methods in the exploration of aeromicrobiome’s composition and functioning.

Graphical abstract
In vivodisentanglement of diffusion frequency-dependence, tensor shape, and relaxation using multidimensional MRI
Authors: Johnson Jessica T.E. , Irfanoglu M. Okan , Manninen Eppu , Ross Thomas J. , Yang Yihong , Laun Frederik B. , Martin Jan , Topgaard Daniel , Benjamini Dan
Published: October 2023

Diffusion MRI with free gradient waveforms, combined with simultaneous relaxation encoding, referred to as multidimensional MRI (MD-MRI), offers microstructural specificity in complex biological tissue. This approach delivers intravoxel information about the microstructure, local chemical composition, and importantly, how these properties are coupled within heterogeneous tissue containing multiple microenvironments. Recent theoretical advances incorporated diffusion time dependency and integrated MD-MRI with concepts from oscillating gradients. This framework probes the diffusion frequency,ω, in addition to the diffusion tensor,D, and relaxation,R1,R2, correlations. AD(ω)-R1-R2clinical imaging protocol was then introduced, with limited brain coverage and 3 mm3voxel size, which hinder brain segmentation and future cohort studies. In this study, we introduce an efficient, sparsein vivoMD-MRI acquisition protocol providing whole brain coverage at 2 mm3voxel size. We demonstrate its feasibility and robustness using a well-defined phantom and repeated scans of five healthy individuals. Additionally, we test different denoising strategies to address the sparse nature of this protocol, and show that efficient MD-MRI encoding design demands a nuanced denoising approach. The MD-MRI framework provides rich information that allows resolving the diffusion frequency dependence into intravoxel components based on theirD(ω)-R1-R2distribution, enabling the creation of microstructure-specific maps in the human brain. Our results encourage the broader adoption and use of this new imaging approach for characterizing healthy and pathological tissues.
The benzoxazinoid breakdown product 5-Methoxy-2-Nitrophenol has a dual function in maize defense against herbivory
Authors: Schimmel Bernardus C. J. , Escobar-Bravo Rocío , Mateo Pierre , Chen Cindy , Glauser Gaétan , Ogi Vera , Wang Lei , Verdecia-Mogena Arletys , Robert Christelle A. M. , Erb Matthias
Published: October 2023

Catabolism of plant secondary metabolites can yield bioactive breakdown products. However, these compounds often remain poorly characterized. Here, we report on the discovery, biosynthesis, and biological relevance of 5-Methoxy-2-Nitrophenol (5M2NP), a secondary metabolite breakdown product which accumulates in damaged maize tissues. We used mutant plants, biochemical complementation, and metabolomic analyses to determine the biosynthetic origin of 5M2NP. Additionally, we assessed 5M2NP’s role in plant defense against herbivores. For this, we surveyed defense-associated responses (gene expression, phytohormones, volatile organic compounds) in wounded, 5M2NP-supplemented maize leaves, and performed bioassays with herbivorous insects. We found that 5M2NP accumulation upon tissue disruption is contingent upon a functional benzoxazinoid biosynthesis pathway. Labeling experiments demonstrated that 5M2NP is derived from DIMBOA. Physiological doses of exogenous 5M2NP increased the wound-induced expression of defense genes and emission of terpenoids. Additionally, 5M2NP exhibited antibiotic and antixenotic activities towards both generalist and specialist herbivores in nano-to micromolar quantities. We conclude that 5M2NP, which represents a novel class of plant-derived compounds, can act as a direct defense and a defense modulator. 5M2NP thus expands the functional repertoire of benzoxazinoids and likely contributes to their protective function against insect herbivores. The discovery of 5M2NP highlights the multifunctionality of plant secondary metabolites and their breakdown products.
The Hidden Diversity of Vascular Patterns in Flower Heads
Authors: Owens Andrew , Zhang Teng , Gu Philmo , Hart Jeremy , Stobbs Jarvis , Cieslak Mikolaj , Elomaa Paula , Prusinkiewicz Przemyslaw
Published: October 2023

Vascular systems are intimately related to the shape and spatial arrangement of the plant organs they support. We investigate the largely unexplored association between spiral phyllotaxis and the vascular system in Asteraceae flowers heads.

We imaged heads of eight species using synchrotron-based X-ray micro-computed tomography and applied original virtual reality and haptic software to explore head vasculature in three dimensions. We then constructed a computational model to infer a plausible patterning mechanism.

The vascular system in the head of the model plantGerbera hybridais qualitatively different from those ofBellis perennisandHelianthus annuus, characterized previously.Cirsium vulgare, Craspedia globosa, Echinacea purpurea, Echinops bannaticus, andTanacetum vulgarerepresent variants of the Bellis and Helianthus systems. In each species the layout of the main strands is stereotypical, but details vary. The observed vascular patterns can be generated by a common computational model with different parameter values.

In spite of the observed differences of vascular systems in heads, they may be produced by a conserved mechanism. The diversity and irregularities of vasculature stand in contrast with the relative uniformity and regularity of phyllotactic patterns, confirming that phyllotaxis in heads is not driven by the vasculature.
Shifts in attention drive context-dependent subspace encoding in anterior cingulate cortex during decision making
Authors: Hajnal Márton Albert , Tran Duy , Szabó Zsombor , Albert Andrea , Safaryan Karen , Einstein Michael , Martelo Mauricio Vallejo , Polack Pierre-Olivier , Golshani Peyman , Orbán Gergő
Published: October 2023

Attention is a cognitive faculty that selects part of a larger set of percepts, driven by cues such as stimulus saliency, internal goals or priors. The enhancement of the attended representation and inhibition of distractors have been proposed as potential neural mechanisms driving this selection process. Yet, how attention operates when the cue has to be internally constructed from conflicting stimuli, decision rules, and reward contingencies, is less understood. Here we recorded from populations of neurons in the anterior cingulate cortex (ACC), an area implicated in ongoing error monitoring and correction during decision conflicts, in a challenging attention-shifting task. In this task, mice had to attend to the rewarded modality when presented identical auditory and visual stimuli in two contexts without direct external cues. In the ACC, the irrelevant stimulus continuously became less decodable than the relevant stimulus as the trial progressed to the decision point. This contrasted strongly with our previous findings in V1 where both relevant and irrelevant stimuli were equally decodable throughout the trial. Using analytical tools and a recurrent neural network (RNN) model, we found that the linearly independent representation of stimulus modalities in ACC was well suited to context-gated suppression of a stimulus modality. We demonstrated that the feedback structure of lateral connections in the RNN consisted of excitatory interactions between cell ensembles representing the same modality and mutual inhibition between cell ensembles representing distinct stimulus modalities. Using this RNN model showing signatures of context-gated suppression, we predicted that the level of contextual modulation of individual neurons should be correlated with their relative responsiveness to the two stimulus modalities used in the task. We verified this prediction in recordings from ACC neurons but not from recordings from V1 neurons. Therefore, ACC effectively operates on low-dimensional neuronal subspaces to combine stimulus related information with internal cues to drive actions under conflict.
Cultivating efficiency: High-throughput growth analysis of anaerobic bacteria in compact microplate readers
Authors: Snoeyenbos-West Oona L.O. , Guerrero Christina R , Valencia Makaela , Carini Paul
Published: October 2023

Anaerobic microbes play crucial roles in environmental processes, industry, and human health. Traditional methods for monitoring the growth of anaerobes, including plate counts or subsampling broth cultures for optical density measurements, are time and resource intensive. The advent of microplate readers revolutionized bacterial growth studies by enabling high-throughput and real-time monitoring of microbial growth kinetics but their use in anaerobic microbiology has remained limited. Here, we present a workflow for using small-footprint microplate readers and the Growthcurver R package to analyze the kinetic growth metrics of anaerobic bacteria. We benchmarked the small-footprint Cerillo Stratus microplate reader against a BioTek Synergy HTX microplate reader in aerobic conditions usingEscherichia coliDSM 28618 cultures. The growth rates and carrying capacities obtained from the two readers were statistically indistinguishable. However, the area under the logistic curve was significantly higher in cultures monitored by the Stratus reader. We used the Stratus to quantify the growth responses of anaerobically grownE. coliandClostridium bolteaeDSM 29485 to different doses of the toxin sodium arsenite. The growth ofE. coliandC. bolteaewas sensitive to arsenite doses of 1.3 μM and 0.4 μM, respectively. Complete inhibition of growth was achieved at 38 μM arsenite forC. bolteae, and 338 μM inE. coli. These results show that the Stratus performs similarly to a leading brand of microplate reader and can be reliably used in anaerobic conditions. We discuss the advantages of the small format microplate readers and our experiences with the Stratus.

Importance statement

We present a workflow that facilitates the production and analysis of growth curves for anaerobic microbes using small-footprint microplate readers and an R script. This workflow is a cost and space-effective solution to most high-throughput solutions for collecting growth data from anaerobic microbes. This technology can be used for applications in which high-throughput would advance discovery, including microbial isolation, bioprospecting, co-culturing, host-microbe interactions, and drug/toxin-microbial interactions.
Shared and Distinct Mechanisms of UBA1 Inactivation Across Different Diseases
Authors: Collins Jason C. , Magaziner Samuel J. , English Maya , Hassan Bakar , Chen Xiang , Balanda Nicholas , Anderson Meghan , Lam Athena , Fernandez-Pol Sebastian , Kwong Bernice , Greenberg Peter L. , Terrier Benjamin , Likhite Mary E. , Kosmider Olivier , Wang Yan , Samara Nadine L. , Walters Kylie J. , Beck David B. , Werner Achim
Published: October 2023

Most cellular ubiquitin signaling is initiated by UBA1, which activates and transfers ubiquitin to tens of E2 enzymes. Clonally acquiredUBA1missense mutations cause an inflammatory-hematologic overlap disease called VEXAS (vacuoles, E1, X-linked, autoinflammatory, somatic) syndrome. Despite extensive clinical investigation into this lethal disease, little is known about the underlying molecular mechanisms. Here, by dissecting VEXAS-causingUBA1mutations, we discovered that p.Met41 mutations alter cytoplasmic isoform expression, whereas other mutations reduce catalytic activity of nuclear and cytoplasmic isoforms by diverse mechanisms, including aberrant oxyester formation. Strikingly, non-p.Met41 mutations most prominently affect transthioesterification, revealing ubiquitin transfer to cytoplasmic E2 enzymes as a shared property of pathogenesis amongst different VEXAS syndrome genotypes. A similar E2 charging bottleneck exists in some lung cancer-associatedUBA1mutations, but not in spinal muscular atrophy-causingUBA1mutations, which instead, render UBA1 thermolabile. Collectively, our results highlight the precision of conformational changes required for faithful ubiquitin transfer, define distinct and shared mechanisms of UBA1 inactivation in diverse diseases, and suggest that specific E1-E2 modules control different aspects of tissue differentiation and maintenance.
Social bond dynamics and the evolution of helping
Authors: Leimar Olof , Bshary Redouna
Published: October 2023

Empiricists often struggle to apply game theory models to real-life cases of animal cooperation. One reason is that many examples of cooperation occur in stable groups, where costs and benefits of helping depend on several factors. Among these are variable investments, fitness interdependencies, learning, memory, reciprocity, and partner choice, including the formation of social bonds with specific group members. Here, we present a game theory model exploring the conditions under which social bonds between group members can promote cooperation, with reciprocal acts of helping. In the model, bonds build up from exchanges of help in a similar way as the strength of association increases in learning, as in the Rescorla-Wagner rule. The bonds in turn affect partner choice and influence helping amounts. The model has a mechanism of reciprocity for bonded pairs, which can evolve towards either loose or strict reciprocation. Several aspects of the model are inspired by observations of food sharing in vampire bats. We find that small social neighbourhoods are required for the evolutionary stability of helping, either as small group sizes, or if members of larger groups can form temporary (daily) smaller groupings. The evolutionary outcome is a fairly low cost helping, while the benefit of receiving help can be substantial. Individuals in need request help based on bond strength, but there is also an evolved preference for initiating bonds with new group members. In contrast, if different groups come into temporary contact with each other, the evolved tendency is to avoid forming bonds between groups.

Popular science summary

The search for evolutionary explanations of cooperation between members of social groups has long been a high profile endeavour. A case of particular interest is when individuals develop a network of friends and exchange help through these social bonds. The question of helping between friends was given emphasis already from the start of the evolutionary study of cooperation, more than 50 years ago, but it has remained without any decisive progress since that time. Here we present a game-theory analysis of helping through the build-up of social bonds. We find that there is reciprocity in socially bonded pairs, which is neither immediate nor very strict, and that relatively small social neighbourhoods are required for the evolutionary stability of helping.
Non-coding variants impactcis-regulatory coordination in a cell type-specific manner
Authors: Pushkarev Olga , van Mierlo Guido , Kribelbauer Judith F. , Saelens Wouter , Gardeux Vincent , Deplancke Bart
Published: October 2023

BACKGROUND

Interactions amongcis-regulatory elements (CREs) play a crucial role in gene regulation. Various approaches have been developed to map these interactions genome-wide, including those relying on interindividual epigenomic variation to identify groups of covariable regulatory elements, referred to as chromatin modules (CMs). While CM mapping allows to investigate the relationship between chromatin modularity and gene expression, the computational principles used for CM identification vary in their application and outcomes.

RESULTS

We comprehensively evaluate and streamline existing CM mapping tools and present guidelines for optimal utilization of epigenome data from a diverse population of individuals to assess regulatory coordination across the human genome. We showcase the effectiveness of our recommended practices by analysing distinct cell types and demonstrate cell type-specificity of CRE interactions in CMs and their relevance for gene expression. Integration of genotype information revealed that many non-coding disease-associated variants affect the activity of CMs in a cell type-specific manner by affecting the binding of cell type-specific transcription factors. We provide example cases that illustrate in detail how CMs can be used to deconstruct GWAS loci, understand variable expression of cell surface receptors in immune cells and reveal how genetic variation can impact the expression of prognostic markers in chronic lymphocytic leukaemia.

CONCLUSIONS

Our study presents an optimal strategy for CM mapping, and reveals how CMs capture the coordination of CREs and its impact on gene expression. Non-coding genetic variants can disrupt this coordination, and we highlight how this may lead to disease predisposition in a cell type-specific manner.
Scale-independent topological interactions drive the first fate decision in theDrosophilaembryo
Authors: Hur Woonyung , Mukherjee Arghyadip , Hayden Luke , Lu Ziqi , Chao Anna , Mitchell Noah P. , Streichan Sebastian J. , Vergassola Massimo , Talia Stefano Di
Published: October 2023

During embryogenesis, the earliest cell fate decision is often linked to nuclear positioning, whose control arises from the integration of the cell cycle oscillator and associated cytoskeletal dynamics. Yet, the mechanisms that ensure that the correct number of nuclei move to the appropriate place remain poorly understood. Here, using light sheet microscopy, we show that inDrosophilaembryos spindle orientation controls which nuclei migrate towards the cortex and which remains inside the embryo, thereby determining nuclear fate and the number of cells undergoing development. Combining computational methods inspired by integral geometry and manipulations of cell cycle genes, we show that spindle orientation is controlled by topological spindle-spindle interactions and not by internuclear distance. Using arguments describing the behavior of space-filling systems, we develop a theory for topological dependency in microtubule structures. Our work shows how topological interplay of microtubule mechanics can ensure robust control of density and cell fate determination.
In vitro reconstitution of SARS-CoV-2 Nsp1-induced mRNA cleavage reveals the key roles of the N-terminal domain of Nsp1 and the RRM domain of eIF3g
Authors: Abaeva Irina S. , Arhab Yani , Miścicka Anna , Hellen Christopher U.T. , Pestova Tatyana V.
Published: October 2023

SARS CoV-2 nonstructural protein 1 (Nsp1) is the major pathogenesis factor that inhibits host translation using a dual strategy of impairing initiation and inducing endonucleolytic cleavage of cellular mRNAs. To investigate the mechanism of cleavage, we reconstituted it in vitro on β-globin, EMCV IRES, and CrPV IRES mRNAs that use unrelated initiation mechanisms. In all instances, cleavage required Nsp1 and only canonical translational components (40S subunits and initiation factors), arguing against involvement of a putative cellular RNA endonuclease. Requirements for initiation factors differed for these mRNAs, reflecting their requirements for ribosomal attachment. Cleavage of CrPV IRES mRNA was supported by a minimal set of components consisting of 40S subunits and eIF3g's RRM domain. The cleavage site was located in the coding region 18 nt downstream from the mRNA entrance, indicating that cleavage occurs on the solvent side of the 40S subunit. Mutational analysis identified a positively charged surface on Nsp1's N-terminal domain (NTD) and a surface above the mRNA-binding channel on eIF3g's RRM domain that contain residues essential for cleavage. These residues were required for cleavage on all three mRNAs, highlighting general roles of the Nsp1 NTD and eIF3g's RRM domain in cleavage per se, irrespective of the mode of ribosomal attachment.
Allelic chromatin structure precedes imprinted expression ofKcnk9during neurogenesis
Authors: Loftus Daniel , Bae Bongmin , Whilden Courtney M. , Whipple Amanda J.
Published: October 2023

Differences in chromatin state inherited from the parental gametes influence the regulation of maternal and paternal alleles in offspring. This phenomenon, known as genomic imprinting, results in genes preferentially transcribed from one parental allele. While local epigenetic factors such as DNA methylation are known to be important for the establishment of imprinted gene expression, less is known about the mechanisms by which differentially methylated regions (DMRs) lead to differences in allelic expression across broad stretches of chromatin. Allele-specific higher-order chromatin structure has been observed at multiple imprinted loci, consistent with the observation of allelic binding of the chromatin-organizing factor CTCF at multiple DMRs. However, whether allelic chromatin structure impacts allelic gene expression is not known for most imprinted loci. Here we characterize the mechanisms underlying brain-specific imprinted expression of thePeg13-Kcnk9locus, an imprinted region associated with intellectual disability. We performed region capture Hi-C on mouse brains from reciprocal hybrid crosses and found imprinted higher-order chromatin structure caused by the allelic binding of CTCF to thePeg13DMR. Using an in vitro neuron differentiation system, we showed that imprinted chromatin structure precedes imprinted expression at the locus. Additionally, activation of a distal enhancer induced imprinted expression ofKcnk9in an allelic chromatin structure-dependent manner. This work provides a high-resolution map of imprinted chromatin structure and demonstrates that chromatin state established in early development can promote imprinted expression upon differentiation.
Apparel behavioural intentions of urban bottom-of-the-pyramid consumers: exploring the role of store atmospherics
Authors: van Niekerk Bianca Maria , Roberts-Lombard Mornay , Cunningham Nicole
Published: October 2023

Purpose

This study aims to explore the impact of store atmospherics on urban bottom-of-the-pyramid consumers’ behavioural intentions to purchase apparel in an emerging African market context. This study also considers purchase antecedents to attitude, perceived behavioural control and social norms as determinants of urban bottom-of-the-pyramid consumers’ apparel behavioural intentions.

Design/methodology/approach

Using non-probability sampling, specifically purposive and interlocking sampling, data collection was secured from 881 economically active Namibian urban bottom-of-the-pyramid respondents through interviewer-administered questionnaires. Covariance-based structural equation modelling assessed the significant relationships among all constructs in the conceptual model.

Findings

This study found that for favourable apparel behavioural intentions of urban bottom-of-the-pyramid consumers to occur, apparel retailers should emphasise trust, perceived awareness and self-identity through apparel assortment and groupings, easy-to-read visible signage, together with competent, friendly and respectful sales personnel in their store atmospherics.

Practical implications

The findings of this study may guide apparel retailers in other emerging African markets to develop regional integration, market-based solutions and inclusive economic growth focusing on “non-essential” products, such as apparel, among urban bottom-of-the-pyramid consumers.

Originality/value

This study expands the intellectual boundaries of urban bottom-of-the-pyramid consumers’ behavioural intentions towards “non-essential” products. The theoretical framework supports the integration of both the stimulus-organism-response model and the theory of planned behaviour into one single model for empirical investigation. Additionally, adopting a novel theoretical framework helped identify the impact of store atmospherics from a bottom-of-the-pyramid perspective in an emerging African market context, such as Namibia.
How does TMT heterogeneity affect breakthrough innovation? The moderating effect of strategic decision-making logics
Authors: Yang Xuecheng , Shao Yunfei
Published: October 2023

Purpose

Drawing on the upper echelons theory, this study focuses on how top management team (TMT) heterogeneity affects breakthrough innovations and examines how strategic decision-making logic (including causation and effectuation) moderates the relationship between TMT heterogeneity and breakthrough innovation.

Design/methodology/approach

By conducting an empirical test of 227 sample firms in China, the authors applied linear hierarchical regression analysis to test the hypotheses on the TMT heterogeneityinnovation relationship and the moderating roles of causation and effectuation.

Findings

The empirical tests show that TMT heterogeneity positively affects breakthrough innovation, and both causation and effectuation positively moderate the positive relationship between TMT heterogeneity and breakthrough innovation. In addition, effectuation has a stronger moderating effect on the positive correlation between TMT heterogeneity and breakthrough innovation than causation.

Originality/value

This study extends the upper echelons theory to explain how the characteristics of TMTs affect firm innovation. Specifically, the authors explore the TMT heterogeneity–breakthrough innovation relationship from the perspectives of information processing and core competence and reveal the boundary condition of strategic decision-making logic in the correlation between TMT heterogeneity and breakthrough innovation. In this vein, the authors contribute to the literature by untangling the internal mechanisms between TMT heterogeneity and breakthrough innovation and extending the discussion on effectuation theory from the entrepreneurship domain to the innovation field. Furthermore, the research findings can provide helpful implications for TMTs to manage breakthrough innovation effectively.
A profile of core workplace skills in developing countries: utilizing PIAAC datasets
Authors: Xu Xiao
Published: October 2023

Purpose

Utilizing datasets of Ecuador, Hungary, Kazakhstan, Mexico and Peru from the Programme for the International Assessment of Adult Competencies survey from 2017 to 2018, this study aimed to develop and validate a profile indicating core workplace skills in developing countries.

Design/methodology/approach

DeVellis' guide of scale development navigated the development of the profile. Multiple techniques including item analysis, exploratory factor analysis, confirmatory factor analysis and multigroup confirmatory factor analysis were used on a sample of 7,166 participants to validate the profile of core workplace skills in developing countries.

Findings

A resultant five-dimensional profile with 18 items was developed: oral communication skills, reading skills, math skills, information and communication technology skills and learning skills. The estimates of composite reliability showed the profile was reliable. The validity estimates of the profile were obtained from several sources including content, convergent, discriminative and construct validity. The measurement invariance was also held for the profile.

Originality/value

Based on the researcher's knowledge, the study is the first attempt to develop a profile to indicate core workplace skills in developing countries. The profile theoretically framed the core workplace skills in developing countries and provides a new measure for identifying, evaluating and thus improving core workplace skills in developing countries for different stakeholders in the era of Education 4.0.
The magic number: three women on the board
Authors: Salaiz Ashley , Faifman Leon
Published: October 2023

Purpose

This study aims to unpack the progress of board gender diversity among the 3,000 largest US listed firms by market capitalization (i.e. Russell 3000 Index). This study extrapolates four classifications of firms based on the number of women in the boardroom: zero women, one or two women, three plus women and gender balanced. The purpose of this study is to examine where progress has and has not been made, why firms plateau and an agenda for the future.

Design/methodology/approach

This study first provides a summative overview of the literature on the benefits of board gender diversity. It then examines progress according to the four classifications, each of which have theoretical underpinnings for whether or not firms can reap the strategic benefits of gender-diverse boardrooms.

Findings

Several indices of US publicly traded companies now have women holding between 30% and 33% of the seats in the boardroom. By examining the spread of women on boards according to the four classifications, this study extrapolates three key insights: firms experiencing tokenism (i.e. one or two women in the boardroom) do not have enough women to reap the strategic benefits of diverse boardrooms; firms that have reached a critical mass (three women in the boardroom) are at an impasse and may risk plateauing; and gender-balanced firms are elevated to the status of being role models for other firms. Calls for action and associated action plans accompany these insights.

Practical implications

This study reminds managers and directors of the strategic benefits of gender-diverse boards and offers three critical insights that boards can use to classify what stage they are at on the path toward board gender equality. Based on their classification, calls for action and action plans offer guidance to firms.

Originality/value

This study shifts away from focusing on the average percentage of board seats held by women across all firms and offers new insights on the progress that firms have made according to the number of women in their boardroom.
At the crossroads: generative AI and corporate risk management
Authors: Hirsch Peter Buell
Published: October 2023

Purpose

This study aims to identify the risks to corporate reputation presented by deepfakes and how to manage them.

Design/methodology/approach

The approach involves a review of the current literature on deepfakes across different sectors to create a clear picture of the risks that deepfakes entail and how best to deal with them.

Findings

While deepfakes are still mostly easily detectable, their sophistication increases daily, and corporations need both technology and culture shifts to deal with this evolving threat.

Originality/value

While deepfakes have been the subject of intense interest, to the best of the author’s knowledge, this is the first attempt to look at the problem from the perspective of corporate enterprise risk.
Uncovering misalignments in the health- and elderly care system
Authors: Hemphälä Jens , Eneberg Magnus
Published: October 2023

Purpose

The increasing size of the elderly population is emerging as a primary catalyst for the escalation of healthcare expenditure, and a sense of urgency is manifest. However, the complexity of the health- and elderly care systems provides challenges in improving system efficiency. Hence, the system-level understanding of the main obstacles to integration care needs further exploration. In order to better integrate health- and elderly care, the study needs to identify the actual misalignments underpinning the issue. This study provides the theoretical foundations for resource misalignments and provides empirical examples of these.

Design/methodology/approach

Semi-structured interviews with multiple stakeholders on various hierarchical levels were carried out to create a more complete view of the system and resources deployed in health- and elderly care. The application of user-centered design methods and co-creation with employees have also been crucial to the outcomes of the study.

Findings

Results show that health- and elderly care is a large-scale complex system. The overlapping and mutually reinforcing misalignments are: (1) regulation and policy differences, (2) stakeholder quantity and variation, (3) external control of health- and elderly care, (3) decreasing collaboration and (4) communication channels and IT development.

Originality/value

This qualitative study builds on institutional theory and resource integration theory and contributes with empirical descriptions of misalignments in the health- and elderly care system. These descriptions will serve as points of departure for systems design to improve the efficiency and effectiveness of health- and elderly care.
An analysis of the wool characteristics that determine wool price in South Africa
Authors: Zenda Mashford , Malan Paul , Geyer Antonie
Published: October 2023

Purpose

South Africa’s wool industry plays an important role in the agricultural sector. The wool industry provides a valuable source of income for farmers who practice sustainable farming practices. However, wool farmers face numerous challenges, such as wool contamination, dirty wool and producing good-quality wool. Good-quality wool is determined by fibre diameter, clean yield, vegetable matter and staple length. This study aims to address these challenges.

Design/methodology/approach

A multiple regression analysis of price (R/kg) of White wool and Merino wool was applied to four variables fibre diameter: vegetable matter, clean yield and staple length. The analysis was based on the data for the 2009–2019 data from Cape Wools auctions.

Findings

Fibre diameter, clean yield and staple length, with exception of vegetable matter, made a statistically significant contribution to the determination of wool price after all other independent variables were controlled for (p < 0.05). A one-unit (micron) increase in fibre diameter resulted in a 0.404-unit decrease in wool price (R/kg). A one-unit (mm) increase in staple length resulted in a 0.022-unit increase in wool price (R/kg). There was no statistically significant association between vegetable matter and wool price. A one-unit increase in clean yield was associated with a 0.111-unit increase in wool price (R/kg).

Research limitations/implications

Since wool fleeces consist of the largest portion of wool shorn from sheep, it is important for wool farmers to focus on wool with low fibre diameter, high clean yield percentage, low percentage of vegetable matter content and good length of the wool.

Practical implications

Since wool fleeces consist of the largest portion of wool shorn from sheep, it is important for wool farmers to focus on wool with low fibre diameter, high clean yield percentage, low percentage of vegetable matter content and good length of the wool.

Social implications

In a developing country such as South Africa, this study is important for the following reason. It is understanding the wool characteristics that have the most significance influence on the determination of wool price for Merino wool and White wool might effectively help the wool farmers to adapt their production systems to improve the wool characteristics that determine wool price.

Originality/value

This study identified a need for a study to be conducted on all wool classes.
An examination of economic growth determined by foreign direct investment and the institutional environment: a subnational perspective
Authors: Goswami Vandana , Goswami Lalit
Published: October 2023

Purpose

The purpose of this paper is to analyse the relationship between foreign direct investment (FDI) inflows and economic growth with a special focus on the institutional environment at the state level. FDI-led economic growth and economic growth-led FDI have two dominant theoretical foundations, but empirical research supports contradictory findings. These perspectives largely ignore the institutional environment, assuming institutions to be background information.

Design/methodology/approach

To examine the causal relationship between FDI, the Granger causality method has been used. The impact of FDI inflows and other institutional factors on economic growth has been examined using the panel data regression method. The principal component analysis (PCA) method has also been used to develop indexes for some variables.

Findings

Results indicate a two-way Granger causality between FDI inflows and economic growth at the state level. Infrastructures, education expenses, labour availability and gross fixed-capital formation (GFCF) are positive and significant determinants, whilst corruption and FDI inflows are leaving negative impact on state-wise economic growth.

Originality/value

This study contributes to the body of the literature in four different ways: first, it empirically examines the trends and patterns of subnational FDI inflow and economic growth disparity in India; second, it examines the causality between FDI and economic growth. Third, with the institution-based paradigm in international business, it investigates how institutional variables affect the expansion of the economy. Fourth, it extends prior research by examining the link at the state level using a large panel data set made up of 29 states and 7 union territories (UTs) over the years 2000–2019.
The structure of the thermal landscape determined behavioural and physiological responses to simulated predation risk
Authors: Rusch Travis W. , Sears Michael W. , Angilletta Michael J.
Published: October 2023

Although predators can deter an animal from regulating its body temperature by basking or shuttling, this response to predation should depend on the spatial distribution of thermal resources.

By simulating predation risk, we showed that movement, thermoregulation and corticosterone of male lizards (Sceloporus jarrovi) depended on the spatial distribution of shade.

Simulated risk caused lizards to move less, thermoregulate worse and circulate more corticosterone than they did without risk. However, a patchier distribution of shade enabled lizards to move more, thermoregulate better and circulate less corticosterone when exposed to a simulated predator.

In the absence of simulated risk, lizards in patchier environments moved less, thermoregulated better and circulated less corticosterone, indicating the distribution of shade also affected the energetic cost of thermoregulation.

This study provides the first test of a spatial theory of thermoregulation under the perceived risk of predation.

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User experience of wearing comfort of reusable versus disposable surgical gowns and environmental perspectives: A cross‐sectional survey
Authors: van Nieuwenhuizen Kim E. , Friedericy Hans J. , van der Linden Sjaak , Jansen Frank Willem , van der Eijk Anne C.
Published: October 2023

Objective

To determine the user experience of wearing comfort of reusable sterile surgical gowns and compare these gowns with conventional disposable surgical gowns.

Design

Cross‐sectional survey.

Setting

An academic hospital in the Netherlands.

Population

Gynaecologists, surgeons, residents and operating room assistants (n=80).

Methods

Quantitative and qualitative data were obtained via a written questionnaire. Participants provided subjective comments and scored the reusable gown on each individual topic with a score from 1 to 5 (1 = unsatisfactory, 2 = moderate, 3 = good, 4 = very good, 5 = excellent) and compared the reusable gown with the conventional disposable alternative (better, equal or worse).

Main outcome measures

Wearing comfort: ventilation and temperature regulation, fit and length, functionality, barrier function and ease of use.

Results

The results of the overall scores of the reusable gown are scored as ‘very good’ (mean 4.3, SD ± 0.5) by its users. Regarding comparison of the gowns, more than 79% (lowest score 79%, highest score 95%) of the participants scored the reusable gown equal or higher on six of seven topics. The topic ‘ease of use’ was scored equal or higher by 59% of the participants. Subjective comments provided information on possible improvements.

Conclusions

The findings of this study demonstrate that there is professional acceptance regarding the utilisation of reusable surgical gowns. To facilitate broader adoption, it is imperative to foster collaboration among suppliers and healthcare institutions. The reusable surgical gown is an environmentally sustainable, safe and comfortable alternative in the operating room.
Effect of material temperatures on quality of fish vermicelli, a noodle‐shaped surimi product
Authors: Cai Libin , Gao Xia , Xiong Shanbai , An Yueqi , Yi Lin , You Juan , Liu Ru
Published: October 2023

The study investigated the effect of material temperatures (15°C, 20°C, 25°C, 30°C, 35°C, and 40°C) on the quality of fish vermicelli (FV) in terms of morphological, cooking, textural, microstructural, and water distribution properties. Results showed that as the material temperatures increased, the surface smoothness, cooking characteristics, and textural properties of FV significantly increased, followed by a decrease. Especially, when the material temperatures were 25°C and 30°C, the FV samples exhibited denser and more continuous gel networks with more embedded starch particles, resulting in decreased cooking loss and improved tensile strength. However, at material temperatures above 35°C, the continuity of the protein phase was disrupted by overswelling starch granules, leading to the worst cooking characteristics and textural properties. Especially at material temperatures of 40°C, the water holding capacity, hardness, and springiness of FV decreased by 25.59%, 73.48%, and 47.98%, respectively, compared to the samples at 25°C. Additionally, the cooking loss increased by 91.40%. In conclusion, the critical material temperature for the quality deterioration of FV was 35°C, and it was recommended to produce FV below this temperature.