bluuebunny/crossref_metadata_2025 huggingface dataset:
Class of hemorrhagic shock is associated with progressive diastolic coronary flow reversal and diminished left ventricular function
Authors: Elansary Noha N. , Stonko David P. , Treffalls Rebecca N. , Abdou Hossam , Madurska Marta J. , Morrison Jonathan J.
Published: December 2022

Introduction: The relationship between coronary artery flow and left ventricular (LV) function during hemorrhagic shock remains unknown. The aim of this study was to quantify coronary artery flow directionality alongside left ventricular function through the four classes of hemorrhage shock.

Methods: Following baseline data collection, swine were exsanguinated into cardiac arrest via the femoral artery using a logarithmic bleed, taking each animal through the four classes of hemorrhagic shock based on percent bleed (class I: 15%; class II: 15%–30%; class III: 30%–40%; class IV: >40%). Telemetry data, left ventricular pressure-volume loops, and left anterior descending artery flow tracings over numerous cardiac cycles were collected and analyzed for each animal throughout.

Results: Five male swine (mean 72 ± 12 kg) were successfully exsanguinated into cardiac arrest. Mean left ventricular end-diastolic volume, end-diastolic pressure, and stroke work decreased as the hemorrhagic shock class progressed (p < 0.001). The proportion of diastole spent with retrograde coronary flow was also associated with class of hemorrhagic shock (mean 5.6% of diastole in baseline, to 63.9% of diastole in class IV; p < 0.0001), worsening at each class from baseline through class IV. Preload recruitable stroke work (PRSW) decreased significantly in classes II through IV (p < 0.001). Systemic Vascular Resistance (SVR) is associated with class of hemorrhage shock (p < 0.001).

Conclusion: With progressive classes of hemorrhagic shock left ventricular function progressively decreased, and the coronary arteries spent a greater proportion of diastole in retrograde flow, with progressively more negative total coronary flow. Preload recruitable stroke work, a load-independent measure of inotropy, also worsened in severe hemorrhagic shock, indicating the mechanism extends beyond the drop in preload and afterload alone.
Upcoming progress of transcriptomics studies on plants: An overview
Authors: Tyagi Parul , Singh Deeksha , Mathur Shivangi , Singh Ayushi , Ranjan Rajiv
Published: December 2022

Transcriptome sequencing or RNA-Sequencing is a high-resolution, sensitive and high-throughput next-generation sequencing (NGS) approach used to study non-model plants and other organisms. In other words, it is an assembly of RNA transcripts from individual or whole samples of functional and developmental stages. RNA-Seq is a significant technique for identifying gene predictions and mining functional analysis that improves gene ontology understanding mechanisms of biological processes, molecular functions, and cellular components, but there is limited information available on this topic. Transcriptomics research on different types of plants can assist researchers to understand functional genes in better ways and regulatory processes to improve breeding selection and cultivation practices. In recent years, several advancements in RNA-Seq technology have been made for the characterization of the transcriptomes of distinct cell types in biological tissues in an efficient manner. RNA-Seq technologies are briefly introduced and examined in terms of their scientific applications. In a nutshell, it introduces all transcriptome sequencing and analysis techniques, as well as their applications in plant biology research. This review will focus on numerous existing and forthcoming strategies for improving transcriptome sequencing technologies for functional gene mining in various plants using RNA- Seq technology, based on the principles, development, and applications.
Integrated analysis of miRNAome transcriptome and degradome reveals miRNA-target modules governing floral florescence development and senescence across early- and late-flowering genotypes in tree peony
Authors: Guo Lili , Li Yuying , Zhang Chenjie , Wang Zhanying , Carlson John E. , Yin Weinlun , Zhang Xiuxin , Hou Xiaogai
Published: December 2022

As a candidate national flower of China, tree peony has extremely high ornamental, medicinal and oil value. However, the short florescence and rarity of early-flowering and late-flowering varieties restrict further improvement of the economic value of tree peony. Specific miRNAs and their target genes engaged in tree peony floral florescence, development and senescence remain unknown. This report presents the integrated analysis of the miRNAome, transcriptome and degradome of tree peony petals collected from blooming, initial flowering, full blooming and decay stages in early-flowering variety Paeonia ostii ‘Fengdan’, an early-flowering mutant line of Paeonia ostii ‘Fengdan’ and late-flowering variety Paeonia suffruticosa ‘Lianhe’. Transcriptome analysis revealed a transcript (‘psu.G.00014095’) which was annotated as a xyloglucan endotransglycosylase/hydrolase precursor XTH-25 and found to be differentially expressed across flower developmental stages in Paeonia ostii ‘Fengdan’ and Paeonia suffruticosa ‘Lianhe’. The miRNA-mRNA modules were presented significant enrichment in various pathways such as plant hormone signal transduction, indole alkaloid biosynthesis, arachidonic acid metabolism, folate biosynthesis, fatty acid elongation, and the MAPK signaling pathway. Multiple miRNA-mRNA-TF modules demonstrated the potential functions of MYB-related, bHLH, Trihelix, NAC, GRAS and HD-ZIP TF families in floral florescence, development, and senescence of tree peony. Comparative spatio-temporal expression investigation of eight floral-favored miRNA-target modules suggested that transcript ‘psu.T.00024044’ and microRNA mtr-miR166g-5p are involved in the floral florescence, development and senescence associated agronomic traits of tree peony. The results might accelerate the understanding of the potential regulation mechanism in regards to floral florescence, development and abscission, and supply guidance for tree peony breeding of varieties with later and longer florescence characteristics.
Sugarcane responses to two strains of Xanthomonas albilineans differing in pathogenicity through a differential modulation of salicylic acid and reactive oxygen species
Authors: Zhao Jian-Ying , Chen Juan , Shi Yang , Fu Hua-Ying , Huang Mei-Ting , Rott Philippe C. , Gao San-Ji
Published: December 2022

Leaf scald caused byXanthomonas albilineansis one of the major bacterial diseases of sugarcane that threaten the sugar industry worldwide. Pathogenic divergence among strains ofX. albilineansand interactions with the sugarcane host remain largely unexplored. In this study, 40 strains ofX. albilineansfrom China were distributed into three distinct evolutionary groups based on multilocus sequence analysis and simple sequence repeats loci markers. In pathogenicity assays, the 40 strains ofX. albilineansfrom China were divided into three pathogenicity groups (low, medium, and high). Twenty-four hours post inoculation (hpi) of leaf scald susceptible variety GT58, leaf populations ofX. albilineansstrain XaCN51 (high pathogenicity group) determined by qPCR were 3-fold higher than those of strain XaCN24 (low pathogenicity group). Inoculated sugarcane plants modulated the reactive oxygen species (ROS) homoeostasis by enhancing respiratory burst oxidase homolog (ScRBOH) expression and superoxide dismutase (SOD) activity and by decreasing catalase (CAT) activity, especially after infection byX. albilineansXaCN51. Furthermore, at 24 hpi, plants infected with XaCN51 maintained a lower content of endogenous salicylic acid (SA) and a lower expression level of SA-mediated genes (ScNPR3,ScTGA4,ScPR1, andScPR5) as compared to plants infected with XaCN24. Altogether, these data revealed that the ROS production-scavenging system and activation of the SA pathway were involved in the sugarcane defense response to an attack byX. albilineans.
Exploration of the rhizosphere microbiome of native plant Ceanothus velutinus – an excellent resource of plant growth-promoting bacteria
Authors: Ganesh Jyothsna , Singh Vishal , Hewitt Katherine , Kaundal Amita
Published: December 2022

Continuous demand for an increase in food production due to climate change and a steady rise in world population requires stress-resilient, sustainable agriculture. Overuse of chemical fertilizers and monoculture farming to achieve this goal deteriorated soil health and negatively affected its microbiome. The rhizosphere microbiome of a plant plays a significant role in its growth and development and promotes the plant’s overall health through nutrient uptake/availability, stress tolerance, and biocontrol activity. The Intermountain West (IW) region of the US is rich in native plants recommended for low water use landscaping because of their drought tolerance. The rhizosphere microbiome of these native plants is an excellent resource for plant growth-promoting rhizobacteria (PGPR) to use these microbes as biofertilizers and biostimulants to enhance food production, mitigate environmental stresses and an alternative for chemical fertilizer, and improve soil health. Here, we isolated, purified, identified, and characterized 64 bacterial isolates from a native plant,Ceanothus velutinus, commonly known as snowbrush ceanothus, from the natural habitat and the greenhouse-grown native soil-treated snowbrush ceanothus plants. We also conducted a microbial diversity analysis of the rhizosphere of greenhouse-grown native soil-treated and untreated plants (control). Twenty-seven of the 64 isolates were from the rhizosphere of the native region, and 36 were from the greenhouse-grown native soil-treated plants. These isolates were also tested for plant growth-promoting (PGP) traits such as their ability to produce catalase, siderophore, and indole acetic acid, fix atmospheric nitrogen and solubilize phosphate. Thirteen bacterial isolates tested positive for all five plant growth-promoting abilities and belonged to the generaPantoea,Pseudomonas,Bacillus, andAncylobacter. Besides, there are isolates belonging to the genusStreptomyces,Bacillus,Peribacillus,Variovorax,Xenophilus,Brevundimonas, andPriestia, which exhibit at least one of the plant growth-promoting activities. This initial screen provided a list of potential PGPR to test for plant health improvement on model and crop plants. Most of the bacterial isolates in this study have a great potential to become biofertilizers and bio-stimulants.
Hybrid knowledge and climate-resilient agriculture practices of the Tharu in the western Tarai, Nepal
Authors: Chaudhary Buddhi R. , Erskine William , Acciaioli Greg
Published: December 2022

Indigenous knowledge can function as a basis of innovation in agriculture because it is not only culturally accepted, but often also environmentally adaptive. The debates and misunderstandings regarding the relations between Indigenous and scientific knowledge are transforming into a trend to integrate all knowledge to deal with complex issues, such as climate change. In this study we explore the understandings of the Tharu people of their farming system in relation to adaptation and mitigation of climate change, based on mixed methods design using both ethnographic analysis of their specific agriculture practices from participant observation and a survey of 229 households in the western Tarai of Nepal. Among our findings is the fit of the traditional agricultural calendar of the Tharu with the labor regimen of agricultural seasons. We found that conservation tillage-oriented agricultural practices, such as relay cropping, including zero-tillage, remain important in the farming system. Although this practice is decreasing, particularly due to the low yield as compared to the conventional tillage system, relay sowing and zero-tillage in the lowlands and uplands remain important for timely crop sowing. Similarly, mixed cropping is prevalent, particularly among small holders, for subsistence-based farming, in part due to higher yield than sole cropping. We conclude that Indigenous knowledge regarding climate and agriculture practices assists making informed decisions for climate-resilient and low emission agriculture. Although some traditional climate-resilient agriculture practices may yield lower profit than those derived from scientific knowledge/methods, the Tharu have therefore embraced “hybrid knowledge”—a combination of Indigenous and scientific knowledge, technology and practice—to balance increased yield and profit maximization with concurrent decreased vulnerability to extreme weather events. We argue that it is not useful to make firm distinctions among traditional, Indigenous and local knowledge in the age of hybridity. This hybridity is evident in the complementarity of the use of improved varieties and scientific agricultural practices for the major grains and the continuing use of landraces for minor crops such as lentils, peas and mustard. However, further research on the sustainable productivity of such practices is required before their widespread dissemination.
A study of cross-border E-commerce research trends: Based on knowledge mapping and literature analysis
Authors: Chen Yongfeng , Li Mengya , Song Jiajie , Ma Xueli , Jiang Yiding , Wu Sainan , Chen Guan Lin
Published: December 2022

As a result of the trend toward economic globalization, the vigorous development of cross-border e-commerce has attracted many scholars to study this field, involving many related fields, such as consumer behavior, advertising, information systems, and supply chain management. Throughout the existing literature, it can be found that most of the research focuses on certain influencing factors of the development of cross-border e-commerce, and there is no systematic and macro- overview of the development trend of research in this field in recent years, nor the integration and analysis of keywords. Given that the research in the field of cross-border e-commerce is fragmented to a large extent, to effectively explore the research trend in this field, we must understand the current situation of cross-border e-commerce. Systematic bibliometric analysis can solve this problem by providing publishing trends and information on various topics. Therefore, based on the scientific database web, this study collected 198 references related to cross-border e-commerce from 2016 to 2021, briefly introduced the current situation and development trend of cross-border e-commerce, visually analyzed and refined the journals, authors, research institutions, countries, publication years, keywords, citations of academic publications in this field, and other key information, and summarized the development trend and path of CEBC in the existing research. It is helpful for researchers to solve the current research gap, understand the future research direction in this field, and help academia establish a strict knowledge system.
The relationship of inclusive climate and peers' attitude on children with disabilities in China: A mediating role of empathy
Authors: Fu Wangqian , Xiao Yawen , Yin Cui , Zhou Tianqi
Published: December 2022

Objective

Peer attitude towards children with disabilities can significantly impact on the successful implementation of inclusive education. The current study examined the attitude of Chinese students toward peers with disabilities in relation to inclusive climate and empathy.

Method

A total of 392 typically developing students (aging from 10 to 17 years old, with average age of 12.7) in primary and middle schools participated in the study by reporting the Inclusive Climate Scale, Peers Attitude to Students with Disabilities Scale, and Balanced Emotional Empathy Scale.

Results

We found that students held a neutral attitude to peers with disabilities, and girls were more acceptable than boys. There were positive relationships among inclusive climate, empathy and attitude to classmates with disabilities. The mediation analysis showed that empathy played a partial mediation role between inclusive climate and the peers' attitude towards children with disabilities.

Conclusion

Implications for improving students' attitude on peers with disabilities were discussed in the paper.
Chinese university students’ conceptions of feedback and the relationships with self-regulated learning, self-efficacy, and English language achievement
Authors: Lu Shasha , Cheng Liying , Chahine Saad
Published: December 2022

In China, under the influence of examination-driven culture and teacher-centered ways of learning, students’ self-regulated learning (SRL) capabilities, self-efficacy, and actual English proficiency are greatly hindered. Given this situation, the Chinese Ministry of Education has promulgated the use of formative assessment in the College English curriculum at the tertiary level since 2004. Feedback, as an integrated part of formative assessment, takes up the largest proportion of the Chinese College English classroom assessment and intends to facilitate SRL and learning. However, whether feedback could facilitate students’ SRL and learning has not been fully investigated in this context in China. Therefore, this study first explored how students self-reported their conceptions of feedback, SRL, and self-efficacy, and second, the relationships among these constructs and their English language achievement in the College English course. A questionnaire was used to collect data on students’ conceptions of feedback, SRL, self-efficacy, and self-perceived English language proficiency. Their English test scores as an indicator of English language achievement were also collected. A total of 538 participants from a university in Northern China participated in this study. Data were analyzed using descriptive statistics, exploratory factor analyses, Pearson correlation analyses, and multiple regression analyses. The results found that Chinese students from the College English course reported a high level of conceptions of teacher and peer feedback, SRL, and self-efficacy, yet a low level of Teacher/Peer Feedback Ignored. For the relationships among these variables, students’ conceptions of feedback contributed to SRL and self-efficacy. Besides, self-efficacy was found to be the strongest predictor for self-perceived English language proficiency and standardized English test scores, both indicators for English language achievement. From the theoretical perspective, this study addressed the research gap in the literature by examining four constructs together, that is, students’ conceptions of feedback, SRL, self-efficacy, and English language achievement within a university context in China. From the pedagogical angle, the results can also support teachers in their feedback practices to facilitate students’ SRL, self-efficacy, and learning.
Exercise is the dominant factor affecting the development of teenagers' eyesight—Based on the Bayesian model averaging
Authors: Liu Zhong-hui , Zhao Meng-fei , Ma Shuai , Li Yin , Sun Zhi-ying , Gao Lei
Published: December 2022

Objective

The model uncertainty may result in inconsistency about the environmental factors of myopia among students, and the Bayesian model average (BMA) is an effective way to eliminate it. We aimed to explore the influencing factors of myopia in primary and middle school students by BMA.

Methods

The data came from the 2021 National Surveillance of Common Diseases and Health Influencing Factors of students. By stratified random cluster sampling, the physical and mental health status of students in Tianjin and the factors affecting their physical health, such as diet, exercise, mental stress, school bullying, sleep time, and internet use, were investigated. The sample consisted of 8,457 primary school students, 8,191 junior middle school students, and 5,901 senior middle school students. Besides the physical examination, we used computer optometry (non-ciliary paralysis) to screen myopia. And we used BMA to select the risk factors through the BMS package in R.

Results

The exercise was the only factor that affected the eyesight of junior and senior middle schoolers by BMA, with the posterior probability of 0.9736 and 0.9762, but not for the primary students. And we failed to select variables that affected eyesight in grades 4–6 of primary school.

Conclusion

The exercise was a strong influencing factor for the eyesight of students in Tianjin's junior and senior middle schools.
Sleep disturbances, depressive symptoms, and cognitive efficiency as determinants of mistakes at work in shift and non-shift workers
Authors: Yeo Hyewon , Lee Jooyoung , Jeon Sehyun , Lee Somi , Hwang Yunjee , Kim Jichul , Kim Seog Ju
Published: December 2022

Introduction

Shift work is known to reduce productivity and safety at work. Previous studies have suggested that a variety of interrelated factors, such as mood, cognition, and sleep, can affect the performance of shift workers. This study aimed to identify potential pathways from depression, sleep, and cognition to work performance in shift and non-shift workers.

Material and methods

Online survey including the Center for Epidemiologic Studies Depression Scale (CES-D), Cognitive Failure Questionnaire (CFQ), and Pittsburgh Sleep Quality Index (PSQI), as well as two items representing work mistakes were administered to 4,561 shift workers and 2,093 non-shift workers. A multi-group structural equation model (SEM) was used to explore differences in the paths to work mistakes between shift and non-shift workers.

Results

Shift workers had higher PSQI, CES-D, and CFQ scores, and made more mistakes at work than non-shift workers. The SEM revealed that PSQI, CES-D, and CFQ scores were significantly related to mistakes at work, with the CFQ being a mediating variable. There were significant differences in the path coefficients of the PSQI and CES-D between shift and non-shift workers. The direct effects of sleep disturbances on mistakes at work were greater in shift workers, while direct effects of depressive symptoms were found only in non-shift workers.

Discussion

The present study found that shift workers made more mistakes at work than non-shift workers, probably because of depressed mood, poor sleep quality, and cognitive inefficiency. Sleep influences work performance in shift workers more directly compared to non-shift workers.
Anti-violence measures developed by ILO and WHO: Analysis of the prevalence of workplace violence and the effects of implementation in a general hospital in China
Authors: Hu Yubing , Luo Qianqian , Li Rui , Zhang Min , Wang Yuming , Su Peipei , Sun Jing , Li Ying , Liu Yujie , Chen Na , Huang Yiming
Published: December 2022

Objective

Workplace violence (WPV) not only affects workers' physical and mental health but also increases turnover rates and social costs. There are no studies that have consistently followed the prevalence of WPV and the effectiveness of anti-violence measures in cross-sectional studies of the same hospital. The main aim of this study was to understand the prevalence of WPV among medical staff in a hospital in China and the effectiveness of implemented anti-violence measures.

Methods

A cross-sectional survey, which was conducted by using a questionnaire developed by the International Labor Organization and the World Health Organization, was administered in a tertiary hospital in China in 2022 to investigate the occurrence of violence in the past year. Valid data were collected from 1,195 respondents via cell phone. WPV was compared between this survey and a baseline survey conducted at the same hospital in 2017 using the same questionnaire. The relationship between years and gender and occupation was explored using the Cochran–Armitage trend test. Descriptive statistics and chi-square tests were used to respond to the effects of the anti-violence interventions.

Results

After the implementation of anti-violence measures, the prevalence of WPV decreased from 48.47 in 2017 to 33.95% in 2021. The recognition of safety measures increased from 80.16 in 2017 to 87.70% in 2021(P < 0.001), improvement of the work environment increased from 56.57 in 2017 to 65.10% in 2021(P < 0.001), restricted of public access increased from 34.36 in 2017 to 55.60% in 2021(P < 0.001), the patient protocols increased from 37.42 in 2017 to 38.40% in 2021, patient screening increased from 32.11 in 2017 to 41.90% in 2021(P < 0.001), and strict restrictions on workplace cash activities from 29.65% in 2017 to 36.00% in 2021(P < 0.05). The percentage of health workers who felt that anti-violence measures were not at all helpful to their current work decreased from 2017 to 2021.

Conclusions

The prevalence of workplace violence has decreased, recognition of interventions has increased, and the proportion of interventions that are perceived by HWs as not helpful at all has decreased. This study demonstrates that the 12 anti-violence measures recommended by the International Labor Organization are applicable in China. Based on this, hospitals should focus on the improvement of the intervention effect of the following measures to further prevent violence.
Popular cleaning systems of bottles reused for traditional food packaging in the city of Yaoundé (Cameroon) and study of their prospective effectiveness on biofilms
Authors: Mouafo Hippolyte T. , Adjele Jorelle J. B. , Hell Raïssa H. , Baomog Annick M. B. , Tchuenchieu Alex D. , Kamgnia Junior A. N. , Manet Linda , Bonny Patrice , Baleba Roger M. M. , Medoua Gabriel N.
Published: December 2022

This study was focused on the collecting and recycling systems of bottles reused for traditional food packaging in the city of Yaoundé and the presence of biofilms in these bottles. A cross-sectional study approved by the Institutional Review Board was conducted in 43 quarters randomly selected in Yaoundé using a semi-structured questionnaire. The target population was producers and street sellers of traditional foods who used recovered bottles for food packaging and had freely signed the informed consent clearance. Then, the recovered bottles cleaned by the participants and ready to be reused as traditional food packaging were sampled and screened for the presence of biofilms. The results showed that 84% of the 162 participants were women. Bottles reused for traditional food packaging were mainly collected from garbage (70.4%). A total of six different cleaning processes were identified from manufacturers of traditional food products. The cleaning processes were significantly (p < 0.05) dependent on the education level and varied from one manufacturer to another. Amongst the identified cleaning processes, those which included soaking and disinfection unit operations were more efficient in biofilms’ removal. Bottles cleaned according to these processes scored the lowest biofilms’ contents. Independently of the cleaning processes, biofilms were detected in all recovered bottles. This might suggest a potential health risk for consumers.
Linking life table and predation rate for evaluating temperature effects on Orius strigicollis for the biological control of Frankliniella occidentalis
Authors: Ren Xiaoyun , Li Xiaowei , Huang Jun , Zhang Zhijun , Hafeez Muhammad , Zhang Jinming , Chen Limin , Zhou Shuxing , Zhang Lisheng , Lu Yaobin
Published: December 2022

Introduction

Orius spp. are generalist predators released in horticultural and agricultural systems to control thrips. Understanding the effects of temperature on the development, predation rate, and population dynamics of Orius is essential for identifying the optimal timing of Orius release for establishing an adequate population to facilitate synchrony with thrips population growth and to prevent thrips outbreaks. The biological control efficiency of natural enemies as well as predator–prey relationships can be precisely described by integrating life table parameters and the predation rate.

Methods

In this study, the demographic features of Orius strigicollis fed on 2nd instar nymphs of western flower thrips (WFT), Frankliniella occidentalis, were compared at 18.5, 23.5, 27, and 33°C using the TWOSEX-MSChart program. The CONSUME-MSChart program was used to examine predation rates under different temperatures (18.5, 23.5, and 27°C).

Results

The results showed no significant difference in fecundity among those reared at 18.5, 23.5, and 27°C, but fecundity at these temperatures was significantly higher than that at 33°C. The intrinsic rate of increase (r), finite rate of increase (λ), and net reproduction rate (R0) were the highest at 27°C. The net predation rate (C0) and transformation rate (Qp) were significantly higher at 18.5°C (C0 = 168.39 prey/predator, Qp = 8.22) and 23.5°C (C0 = 140.49 prey/predator, Qp = 6.03) than at 27°C (C0 = 138.39 prey/predator, Qp= 3.81); however, the finite predation rate (ω) showed the opposite trend. In addition to temperature, the stage of O. strigicollis at release can affect population dynamics.

Discussion

Our study showed that temperature influenced the demographic traits and predation rates of O. strigicollis. When planning a release, the stage of O. strigicollis and temperature should be taken into account to establish an adequate population for the control of WFT.
Bariatric surgery in liver cirrhosis
Authors: Mehdorn A. S. , Moulla Y. , Mehdorn M. , Dietrich A. , Schönfels W. , Becker T. , Braun F. , Beckmann J. H. , Linecker M.
Published: December 2022

Introduction

Obesity is frequently associated with its hepatic manifestation, the nonalcoholic fatty liver disease (NAFLD). The most effective treatment for morbid obesity is bariatric surgery (BS) also improving NAFLD and liver function. In patients where NAFLD has already progressed to liver cirrhosis, BS can be considered a high-risk procedure. Hence, consideration of the procedure and the most appropriate timing is crucial.

Material and Methods

Obese patients suffering from NAFLD who underwent BS from two German University Medical Centers were retrospectively analyzed.

Results

Twenty-seven patients underwent BS. Most common procedures were laparoscopic Roux-en-Y-gastric (RYGB) and laparoscopic sleeve gastrectomy (SG). All patients suffered from liver cirrhosis Child A. A preoperative transjugular portosystemic shunt (TIPS) was established in three patients and failed in another patient. Postoperative complications consisted of wound healing disorders (n = 2), anastomotic bleeding (n = 1), and leak from the staple line (n = 1). This patient suffered from intraoperatively detected macroscopic liver cirrhosis. Excess weight loss was 73% and 85% after 1 and 2 years, respectively. Two patients suffered from postoperative aggravation of their liver function, resulting in a higher Child–Pugh score, while three could be removed from the waiting list for a liver transplantation.

Conclusion

BS leads to weight loss, both after SG and RYGB, and potential improvement of liver function in liver cirrhosis. These patients need to be considered with care when evaluated for BS. Preoperative TIPS implantation may reduce the perioperative risk in selected patients.
Dietary Lactobacillus fermentum and Lactobacillus paracasei improve the intestinal health of broilers challenged with coccidia and Clostridium perfringens
Authors: Li Peng , Zheng Liyun , Qi Ya , Liu Zhipeng , Du Encun , Wei Jintao , Zhang Zhengfan , Guo Shuangshuang , Ding Binying
Published: December 2022

Necrotic enteritis (NE) is a great threat to the intestinal health of broilers, resulting in decreased growth performance and significant economic losses. Lactobacillus fermentum (LF) and Lactobacillus paracasei (LP) exert beneficial effects on intestinal health. The aim of the present study was to investigate the effects of dietary LF and LP on the intestinal health and growth performance of broilers challenged with coccidia and Clostridium perfringens (CCP). The animal trial was carried out using 336 broilers (Ross 308) for 35 days with a completely randomized design. The broilers were divided into 4 groups based on treatment as follows: the control (CTR) group was fed the basal diet and without CCP challenge and the CCP group was fed the basal diet and with CCP challenge. The broilers in the CCP+LF and CCP+LP groups were challenged by CCP, and meanwhile, LF (1 × 109 CFU/g) and LP (1 × 109 CFU/g) were supplemented into the basal diets, respectively. The results showed that the growth performance and the intestinal morphology were negatively affected by the CCP challenge. In addition, the number of coccidia in the intestinal digesta and the relative abundance of Escherichia coli in the cecal digesta were increased. Besides, the mRNA level of IgA in the jejunum was downregulated, and the transcript level of IL-8 was upregulated by the CCP challenge. Dietary LF and LP failed to improve the growth performance of broilers with the CCP challenge. However, they were beneficial for intestinal barrier function. In addition, dietary LF was able to alleviate the downregulation of TGF-β mRNA level in the spleen with CCP challenge and decreased the lesion scores compared with the CCP group. Furthermore, dietary LP alleviated the upregulation of the IL-8 mRNA level in the jejunum with CCP challenge and reduced the number of coccidia in the ileal digesta. In conclusion, dietary LF and LP failed to mitigate the negative effects of CCP infection on growth performance; however, they were able to improve the intestinal health of broilers challenged with CCP by strengthening the intestinal barrier and alleviating inflammation.
The pharmacokinetics of levobupivacaine 0.5% after infraorbital or inferior alveolar block in anesthetized dogs
Authors: Pavlica Matic , Kržan Mojca , Nemec Ana , Kosjek Tina , Baš Anže , Seliškar Alenka
Published: December 2022

Introduction

Data are lacking on the pharmacokinetic profile and safety of levobupivacaine (LB) used for regional anesthesia of the maxilla and mandibles in dogs.

Methods

Infraorbital block (n= 10), inferior alveolar block (n= 10) or both infraorbital and inferior alveolar blocks (n= 10) were administered to dogs undergoing dental surgery under isoflurane anesthesia. The dose of LB was calculated as 0.11 ml/kg2/3for the infraorbital block and 0.18 ml/kg2/3for the inferior alveolar block. Blood samples were collected before and immediately after administration of the oral blocks, and 3, 4, 7, 12, 17, 32, 47, 62, 92, and 122 min thereafter. Quantification of LB in plasma was performed by LC-MS/MS.

Results and discussion

The results are presented as median and interquartile range. In dogs in which all four quadrants of the oral cavity were desensitized with LB, theCmaxwas 1,335 (1,030–1,929) ng/ml, theTmaxwas 7 (4–9.5) min, and the AUC(0 → 120)was 57,976 (44,954–96,224) ng min/ml. Plasma concentrations of LB were several times lower than the reported toxic concentrations, and no signs of cardiovascular depression or neurotoxicity were observed in any of the dogs, suggesting that the occurrence of severe adverse effects after administration of LB at the doses used in this study is unlikely.
Pharmacokinetics and tolerability of the dual TORC1/2 inhibitor sapanisertib in combination with the MEK inhibitor trametinib in dogs
Authors: Wei Bih-Rong , Peer Cody J. , Richardson William J. , Hewitt Stephen M. , Figg William D. , Simpson R. Mark
Published: December 2022

Activation of one or both the Ras/MAPK and PI3K/Akt/mTOR signal transduction pathways are known to mediate oncogenicity of several canine and human cancers, including mucosal melanomas. Reciprocal cross activation between the two pathways can be a source of drug resistance. Consequently, oral dosing for plasma pharmacokinetic (PK) analysis and tolerability to a combination of sapanisertib, a dual TORC1/2 inhibitor, and trametinib, a MEK inhibitor, was evaluated in nontumor-bearing laboratory dogs for its potential application in parallel pathway targeting. Twelve dogs, divided into three equal cohorts, received either the combination or single agents. Animals were monitored for PK following single dose and 17-day repeat dosing, and by clinical observations, hematology, serum biochemistry, coagulation studies and urinalyses. A single trametinib dose (0.025 mg/kg), sulfated as dimethyl sulfoxide which enhanced its absorption, reached mean maximum concentration (Cmax) 0.64 ng/mL [18% coefficient of variation (CV)] at a median time to maximum concentration (Tmax) of 1.5 h (hr), and mean area under the concentration-time curve (AUC) 16.8 hr*ng/mL (14%CV), which were similar when given alone or in combination with sapanisertib. A prolonged half-life afforded 3–4-fold plasma accumulation of trametinib with daily dosing, analogous to humans. Trametinib PK mirrored previous regulatory data in dogs, while exposure approximated some published human values but generally not all patients. Sapanisertib-alone in canine plasma following single 0.1 mg/kg dose [mean Cmax 26.3 ng/mL (21%CV), median Tmax 2.0 hr, and mean AUC 248 hr*ng/mL (41%CV)] resembled levels in human therapeutic trials; whereas canine sapanisertib exposure was reduced when combined with trametinib, a known cytochrome P450 CYP3A4 inducer. Sex differences were not observed for either drug. Side effects upon repeat dosing with either or both drugs may include body weight loss, maldigestion, and cutaneous discoloration. The combination was tolerated without dose limiting toxicity, although clinical laboratory analyses revealed drug-induced acute-phase inflammation, proteinuria, and decreased blood reticulocytes, mild changes not necessitating intervention. Short-term results in dogs with this combination would appear to hold translational promise for clinical trial evaluation to target canine and possibly human melanoma, as well as other cancers having one or both signal transduction pathway activations.
Pharmacokinetics of esomeprazole in goats (Capra aegagrus hircus) after intravenous and subcutaneous administration
Authors: Fladung Rachel , Smith Joe S. , Hines Melissa T. , Soto-Gonzalez Windy Michelle , Fayne Bryanna , Rahn Rebecca R. , Escher Olivia Grace , Harvill Lainey , Bergman Joan , Garcia Jessica D. , Kreuder Amanda Jo , Cox Sherry
Published: December 2022

Background

Stressed and hospitalized goats are at risk of developing abomasal (gastric) ulceration, but there is a paucity of pharmacokinetic studies for proton pump inhibiting drugs, such as, esomeprazole in goats.

Objectives

The objectives for this study were to estimate plasma pharmacokinetic parameters for esomeprazole in adult goats after intravenous (IV) and subcutaneous (SQ) administration. A secondary objective was to describe the plasma kinetics of the metabolite esomeprazole sulfone after IV and SC administration in goats.

Materials and methods

Esomeprazole was administered to 5 adult goats in a crossover study at doses of 1 mg/kg IV or 2 mg/kg SC. Plasma samples were collected over 36 h and analyzed via reverse phase HPLC to determine concentrations of esomeprazole and esomeprazole sulfone. Pharmacokinetic parameters were derived via non-compartmental analysis.

Results

Following IV administration, mean values for plasma clearance (Cl), elimination half-life [T1/2 (λz)], C0, and volume of distribution (Vz) of esomeprazole were estimated at 24.9 mL/min/kg, 6 min, 2.324 μg/mL, and 0.23 L/kg, respectively. After SC administration elimination half-life, maximum concentration (Cmax) and time to maximum concentration (Tmax) of esomeprazole were estimated at 29 min, 1.038 μg/mL, and 22 minutes respectively. Maximum concentrations of the sulfone metabolite were 32 and 18 ng/mL after IV and SC administration.

Conclusion

Esomeprazole was rapidly eliminated from plasma after both IV and SC injection in goats. The elimination half-life in goats appears to be shorter than reported in dogs, as well as less than that reported for pantoprazole in goats. The sulfone metabolite was detected and also rapidly eliminated from the plasma after both IV and SC administration. Additional pharmacodynamic investigations are needed to determine the efficacy of esomeprazole on abomasal (gastric) acid suppression in goats and could include larger doses or additional routes of administration.
Helicopter Rescue for Flood Disaster: Scheduling, Simulation, and Evaluation
Authors: Xue Yuanbo , Gao Yuan , Tian Yongliang , Liu Hu , Wang Xiyu
Published: December 2022

Frequent severe floods have caused great losses to urban safety and the economy, which raises high requirements for the efficiency and effectiveness of emergency rescue. Due to the flood characteristics, flood rescue requires a more rapid responder and decision-making compared with other kinds of disaster rescue. In recent years, aviation emergency rescue (AER) has attracted much attention for flood applications. In order to evaluate the effectiveness of AER for flood disasters, the present study proposes a conceptual model of helicopter AER scheduling and develops a simulation system of helicopter AER scheduling using multiple agents. Seven elements are considered in the conceptual model: helicopters, the command-and-control center, temporary take-off/landing points, mission demand points, resettlement points, loading points, and unloading points. Furthermore, process-oriented and object-oriented scheduling rules are developed as the general guide for scheduling. In order to efficiently simulate and evaluate an AER mission (assisting the decision maker), the simulation system is designed with multiple agents and a user interface, which can quickly load mission settings, run the simulation, and collect data for further evaluation. A standardized mission makespan is adopted as the evaluation index. Based on that, the minimum integrated index can be derived to finally assess the different rescue schemes and choose the best. In the case study, the comparison results indicate that the rescue efficiency of large helicopters (Mi-26 in the case) could be limited by the capabilities of loading points and unloading points. This problem is solved by scheduling small/medium-size helicopters to transfer the personnel. Alternately, two types of helicopters can be used: one for passenger transfer and the other for goods/material transfer. Anyway, the analyses in the case study illustrate the correlation between effectiveness and scheduling, which demonstrates the significance of decision-making. By using the proposed scheduling and modeling methods, the simulation system can be served as a convenient decision-making support tool for practical rescue applications.
Potential of Omics to Control Diseases and Pests in the Coconut Tree
Authors: Tzec-Simá Miguel , Félix Jean Wildort , Granados-Alegría María , Aparicio-Ortiz Mónica , Juárez-Monroy Dilery , Mayo-Ruiz Damian , Vivas-López Saraí , Gómez-Tah Rufino , Canto-Canché Blondy , Berezovski Maxim V. , Islas-Flores Ignacio
Published: December 2022

The coconut palm (Cocos nucifera L.) is a common crop in pantropical areas facing various challenges, one of them being the control of diseases and pests. Diseases such as bud rot caused by Phytophthora palmivora, lethal yellowing caused by phytoplasmas of the types 16SrIV-A, 16SrIV-D or 16SrIV-E, among others, and pests like the coconut palm weevil, Rhynchophorus vulneratus (Coleoptera: Curculionidae), and the horned beetle, Oryctes rhinocerus (Coleoptera: Scarabaeidae), are controlled by applying pesticides, pheromones and cultural control. These practices do not guarantee eradication since some causal agents have become resistant or are imbedded in infected tissues making them difficult to eradicate. This review condenses the current genomics, transcriptomics, proteomics and metabolomics studies which are being conducted with the aim of understanding the pathosystems associated with the coconut palm, highlighting the findings generated by omics studies that may become future targets for the control of diseases and pests in the coconut crop.
Environmental Simulation Model for Rapid Prediction of Tea Seedling Growth
Authors: Li He , Mao Yilin , Wang Yu , Fan Kai , Shi Hongtao , Sun Litao , Shen Jiazhi , Shen Yaozong , Xu Yang , Ding Zhaotang
Published: December 2022

Accurate and effective monitoring of environmental parameters in tea seedling greenhouses is an important basis for regulating the seedling environment, which is crucial for improving the seedling growth quality. This study proposes a tea seedling growth simulation (TSGS) model based on deep learning. The Internet of Things system was used to measure environmental change during the whole seedling process. The correlation between the environmental parameters and the biomass growth of tea seedlings in various varieties was analyzed. A CNN-LSTM network was proposed to build the TSGS model of light, temperature, water, gas, mineral nutrition, and growth biomass. The results showed that: (1) the average correlation coefficients of air temperature, soil temperature, and soil moisture with the biomass growth of tea seedlings were 0.78, 0.84, and −0.63, respectively, which were three important parameters for establishing the TSGS model. (2) For evaluating the TSGS model of a single variety, the accuracy of ZM’s TSGS based on the CNN-LSTM network was the highest (Rp2 = 0.98, RMSEP = 0.14). (3) For evaluating the TSGS model of multiple varieties, the accuracy of TSGS based on the CNN-LSTM network was the highest (Rp2 = 0.96, RMSEP = 0.17). This study provided effective technical parameters for intelligent control of tea-cutting growth and a new method for rapid breeding.
Oxidation Behavior, Insulation Resistance, and Permeability of FeSiCr Alloys for Multilayer Inductors
Authors: Priese Christoph , Töpfer Jörg
Published: December 2022

FeSiCr alloys are used as soft magnetic materials for power multilayer inductors. The alloys are typically annealed at intermediate temperatures in air during inductor fabrication to form an insulating chromium oxide layer around the alloy particles. The variation of the annealing temperature between 700 °C and 900 °C in air, and, for the first time, the variation of the oxygen partial pressure during annealing at 900 °C are studied, and their effects on the alloy’s oxidation behavior, phase formation, insulation resistance, and permeability are demonstrated. The chromium oxide content increases up to about 12 wt% with annealing temperature in air, whereas it decreases to 8.2 wt% after annealing at 900 °C and 0.001% O2. The observed mass changes during annealing confirm the various tendencies towards oxidation. This oxidation behavior is reflected in an increase in the insulation resistance with annealing temperature or in a resistance reduction with decreasing oxygen partial pressure. The permeability decreases from µ = 22 after annealing at 700 °C to µ = 18.5 at 900 °C in air. The reduction of pO2 during annealing at 900 °C leads to an increase in permeability up to µ = 22.5 at pO2 = 0.001% O2. The results can be used to design cofiring strategies using reduced oxygen partial pressure for new composite multilayer inductive components consisting of FeSiCr- and ferrite layers in combination with silver metallization.