bluuebunny/crossref_metadata_2025 huggingface dataset:
Urbanization Drives SOC Accumulation, Its Temperature Stability and Turnover in Forests, Northeastern China
Authors: Zhai Chang , Wang Wenjie , He Xingyuan , Zhou Wei , Xiao Lu , Zhang Bo
Published: April 2017

Global urbanization is a vital process shaping terrestrial ecosystems but its effects on forest soil carbon (C) dynamics are still not well defined. To clarify the effects of urbanization on soil organic carbon (SOC) variation, 306 soil samples were collected and analyzed under two urban–rural gradients, defined according to human disturbance time and ring road development in Changchun, northeast China. Forest SOC showed a linear increase with increasing human disturbance time from year 1900 to 2014 (13.4 g C m−2 year−1), and a similar trend was found for the ring road gradient. Old-city regions had the longest SOC turnover time and it increased significantly with increasing urbanization (p = 0.011). Along both urban–rural gradients SOC stability toward temperature variation increased with increasing urbanization, meaning SOC stability in old-city regions was higher than in new regions. However, none of the urban–rural gradients showed marked changes in soil basal respiration rate. Both Pearson correlation and stepwise regression proved that these urbanization-induced SOC patterns were closely associated with landscape forest (LF) proportion and soil electrical conductivity (EC) changes in urban–rural gradients, but marginally related with tree size and compositional changes. Overall, Changchun urbanization-induced SOC accumulation was 60.6–98.08 thousand tons, accounting for 12.8–20.7% of the total forest C biomass sequestration. Thus, China’s rapid urbanization-induced SOC sequestration, stability and turnover time, should be fully estimated when evaluating terrestrial C balance.
Red Alder-Conifer Stands in Alaska: An Example of Mixed Species Management to Enhance Structural and Biological Complexity
Authors: Deal Robert , Orlikowska Ewa , D’Amore David , Hennon Paul
Published: April 2017

There is worldwide interest in managing forests to improve biodiversity, enhance ecosystem services and assure long-term sustainability of forest resources. An increasingly important goal of forest management is to increase stand diversity and improve wildlife and aquatic habitat. Well-planned silvicultural systems containing a mixture of broadleaf-conifer species have potential to enhance stand diversity and provide other ecosystem services earlier than typical even-aged conifer plantations. Here, we use the example of mixed Sitka spruce/western hemlock and red alder in young, managed stands in southeast Alaska to achieve these goals. We briefly describe the silvics of Sitka spruce, western hemlock and red alder plantations as pure conifer stands or pure broadleaf stands. Then, we synthesize studies of mixed red alder-Sitka spruce/western hemlock stands in southeast Alaska and present their potential for improving stand structural complexity, biodiversity and other ecosystem services over pure conifer forests. Finally, we discuss some of the opportunities and potential tradeoffs for managing mixed broadleaf-conifer stands for providing a number of natural resources and the influence of these broadleaf-conifer forests on ecosystem linkages and processes.
A Sequential Steam Explosion and Reactive Extrusion Pretreatment for Lignocellulosic Biomass Conversion within a Fermentation-Based Biorefinery Perspective
Authors: Oliva José , Negro María , Manzanares Paloma , Ballesteros Ignacio , Chamorro Miguel , Sáez Felicia , Ballesteros Mercedes , Moreno Antonio
Published: April 2017

The present work evaluates a two-step pretreatment process based on steam explosion and extrusion technologies for the optimal fractionation of lignocellulosic biomass. Two-step pretreatment of barley straw resulted in overall glucan, hemicellulose and lignin recovery yields of 84%, 91% and 87%, respectively. Precipitation of the collected lignin-rich liquid fraction yielded a solid residue with high lignin content, offering possibilities for subsequent applications. Moreover, hydrolysability tests showed almost complete saccharification of the pretreated solid residue, which when combined with the low concentration of the generated inhibitory compounds, is representative of a good pretreatment approach. Scheffersomyces stipitis was capable of fermenting all of the glucose and xylose from the non-diluted hemicellulose fraction, resulting in an ethanol concentration of 17.5 g/L with 0.34 g/g yields. Similarly, Saccharomyces cerevisiae produced about 4% (v/v) ethanol concentration with 0.40 g/g yields, during simultaneous saccharification and fermentation (SSF) of the two-step pretreated solid residue at 10% (w/w) consistency. These results increased the overall conversion yields from a one-step steam explosion pretreatment by 1.4-fold, showing the effectiveness of including an extrusion step to enhance overall biomass fractionation and carbohydrates conversion via microbial fermentation processes.
Validation of a Novel Technique and Evaluation of the Surface Free Energy of Food
Authors: Senturk Parreidt Tugce , Schmid Markus , Hauser Carolin
Published: April 2017

Characterizing the physical properties of a surface is largely dependent on determining the contact angle exhibited by a liquid. Contact angles on the surfaces of rough and irregularly-shaped food samples are difficult to measure using a contact angle meter (goniometer). As a consequence, values for the surface energy and its components can be mismeasured. The aim of this work was to use a novel contact angle measurement method, namely the snake-based ImageJ program, to accurately measure the contact angles of rough and irregular shapes, such as food samples, and so enable more accurate calculation of the surface energy of food materials. In order to validate the novel technique, the contact angles of three different test liquids on four different smooth polymer films were measured using both the ImageJ software with the DropSnake plugin and the widely used contact angle meter. The distributions of the values obtained by the two methods were different. Therefore, the contact angles, surface energies, and polar and dispersive components of plastic films obtained using the ImageJ program and the Drop Shape Analyzer (DSA) were interpreted with the help of simple linear regression analysis. As case studies, the superficial characteristics of strawberry and endive salad epicarp were measured with the ImageJ program and the results were interpreted with the Drop Shape Analyzer equivalent according to our regression models. The data indicated that the ImageJ program can be successfully used for contact angle determination of rough and strongly hydrophobic surfaces, such as strawberry epicarp. However, for the special geometry of droplets on slightly hydrophobic surfaces, such as salad leaves, the program code interpolation part can be altered.
Erratum: Tingting Lian, et al. Identification of Site-Specific Stroke Biomarker Candidates by Laser Capture Microdissection and Labeled Reference Peptide. Int. J. Mol. Sci. 2015, 16, 13427–13441
Authors: Lian Tingting , Qu Daixin , Zhao Xu , Yu Lixia , Gao Bing
Published: April 2017

The authors wish to make a change to their published paper [1]. Since 2013, the authors have performed multiple experiments in many animals, including mice and rats, at both the Sun Yat-Sen University (Guangzhou, China) and China Medical University (Shenyang, China) [...]
Assembling Deep Neural Networks for Medical Compound Figure Detection
Authors: Yu Yuhai , Lin Hongfei , Meng Jiana , Wei Xiaocong , Zhao Zhehuan
Published: April 2017

Compound figure detection on figures and associated captions is the first step to making medical figures from biomedical literature available for further analysis. The performance of traditional methods is limited to the choice of hand-engineering features and prior domain knowledge. We train multiple convolutional neural networks (CNNs), long short-term memory (LSTM) networks, and gated recurrent unit (GRU) networks on top of pre-trained word vectors to learn textual features from captions and employ deep CNNs to learn visual features from figures. We then identify compound figures by combining textual and visual prediction. Our proposed architecture obtains remarkable performance in three run types—textual, visual and mixed—and achieves better performance in ImageCLEF2015 and ImageCLEF2016.
Network Models for Cognitive Development and Intelligence
Authors: Van Der Maas Han , Kan Kees-Jan , Marsman Maarten , Stevenson Claire E.
Published: April 2017

Cronbach’s (1957) famous division of scientific psychology into two disciplines is still apparent for the fields of cognition (general mechanisms) and intelligence (dimensionality of individual differences). The welcome integration of the two fields requires the construction of mechanistic models of cognition and cognitive development that explain key phenomena in individual differences research. In this paper, we argue that network modeling is a promising approach to integrate the processes of cognitive development and (developing) intelligence into one unified theory. Network models are defined mathematically, describe mechanisms on the level of the individual, and are able to explain positive correlations among intelligence subtest scores—the empirical basis for the well-known g-factor—as well as more complex factorial structures. Links between network modeling, factor modeling, and item response theory allow for a common metric, encompassing both discrete and continuous characteristics, for cognitive development and intelligence.
Sediment Transport into the Swinomish Navigation Channel, Puget Sound—Habitat Restoration versus Navigation Maintenance Needs
Authors: Khangaonkar Tarang , Nugraha Adi , Hinton Steve , Michalsen David , Brown Scott
Published: April 2017

The 11 mile (1.6 km) Swinomish Federal Navigation Channel provides a safe and short passage to fishing and recreational craft in and out of Northern Puget Sound by connecting Skagit and Padilla Bays, US State abbrev., USA. A network of dikes and jetties were constructed through the Swinomish corridor between 1893 and 1936 to improve navigation functionality. Over the years, these river training dikes and jetties designed to minimize sedimentation in the channel have deteriorated, resulting in reduced protection of the channel. The need to repair or modify dikes/jetties for channel maintenance, however, may conflict with salmon habitat restoration goals aimed at improving access, connectivity and brackish water habitat. Several restoration projects have been proposed in the Skagit delta involving breaching, lowering, or removal of dikes. To assess relative merits of the available alternatives, a hydrodynamic model of the Skagit River estuary was developed using the Finite Volume Community Ocean Model (FVCOM). In this paper, we present the refinement and calibration of the model using oceanographic data collected from the years 2006 and 2009 with a focus on the sediment and brackish water transport from the river and Skagit Bay tide flats to the Swinomish Channel. The model was applied to assess the feasibility of achieving the desired dual outcome of (a) reducing sedimentation and shoaling in the Swinomish Channel and (b) providing a direct migration pathway and improved conveyance of freshwater into the Swinomish Channel. The potential reduction in shoaling through site-specific structure repairs is evaluated. Similarly, the potential to significantly improve of brackish water habitat through dike breach restoration actions using the McGlinn Causeway project example, along with its impacts on sediment deposition in the Swinomish Navigation Channel, is examined.
Narrative Perspectives on Self-Directed Foreign Language Learning in a Computer- and Mobile-Assisted Language Learning Context
Authors: Isbell Daniel , Rawal Hima , Oh Rachelle , Loewen Shawn
Published: April 2017

Millions of learners around the world use self-directed computer- and mobile-assisted language learning (CALL, MALL) programs to study foreign languages. One such program, Duolingo, currently attracts over 120 million users and is claimed (by the publisher) to be a highly effective method of language learning. While L2 researchers have shown limited engagement with similar large-scale commercial programs, issues related to learner persistence, motivation, and program efficacy have been reported. This study investigates the experiences and efficacy of learning Turkish on Duolingo for 12 weeks, drawing on a methodological tradition of researcher narratives. Three graduate student researchers kept diaries and completed weekly reflections on their Turkish learning experiences, which served as source material for individual narrative analysis. The resulting narratives were discussed and analyzed collaboratively from an ecological perspective. Strategies used by the researcher-participants were heavily influenced by ecological factors. Persistence in learning was found to be influenced by ecological factors and varied across timescales. Ultimately, the researcher-participants had limited Turkish learning outcomes and felt demotivated to continue studying on Duolingo. Implications for CALL/MALL design include presenting materials in a meaningful context, capitalizing on social affordances, and providing meaningful feedback to learners in order to facilitate learning and goal-setting.
Strain Localization of Elastic-Damaging Frictional-Cohesive Materials: Analytical Results and Numerical Verification
Authors: Wu Jian-Ying , Cervera Miguel
Published: April 2017

Damage-induced strain softening is of vital importance for the modeling of localized failure in frictional-cohesive materials. This paper addresses strain localization of damaging solids and the resulting consistent frictional-cohesive crack models. As a supplement to the framework recently established for stress-based continuum material models in rate form (Wu and Cervera 2015, 2016), several classical strain-based damage models, expressed usually in total and secant format, are considered. Upon strain localization of such damaging solids, Maxwell’s kinematics of a strong (or regularized) discontinuity has to be reproduced by the inelastic damage strains, which are defined by a bounded characteristic tensor and an unbounded scalar related to the damage variable. This kinematic constraint yields a set of nonlinear equations from which the discontinuity orientation and damage-type localized cohesive relations can be derived. It is found that for the “Simó and Ju 1987” isotropic damage model, the localization angles and the resulting cohesive model heavily depend on lateral deformations usually ignored in classical crack models for quasi-brittle solids. To remedy this inconsistency, a modified damage model is proposed. Its strain localization analysis naturally results in a consistent frictional-cohesive crack model of damage type, which can be regularized as a classical smeared crack model. The analytical results are numerically verified by the recently-proposed mixed stabilized finite element method, regarding a singly-perforated plate under uniaxial tension. Remarkably, for all of the damage models discussed in this work, the numerically-obtained localization angles agree almost exactly with the closed-form results. This agreement, on the one hand, consolidates the strain localization analysis based on Maxwell’s kinematics and, on the other hand, illustrates versatility of the mixed stabilized finite element method.
Mechanics of Pickering Drops Probed by Electric Field–Induced Stress
Authors: Mikkelsen Alexander , Dommersnes Paul , Rozynek Zbigniew , Gholamipour-Shirazi Azarmidokht , Carvalho Marcio da Silveira , Fossum Jon Otto
Published: April 2017

Fluid drops coated with particles, so-called Pickering drops, play an important role in emulsion and capsule applications. In this context, knowledge of mechanical properties and stability of Pickering drops are essential. Here we prepare Pickering drops via electric field-driven self-assembly. We use direct current (DC) electric fields to induce mechanical stress on these drops, as a possible alternative to the use of, for example, fluid flow fields. Drop deformation is monitored as a function of the applied electric field strength. The deformation of pure silicone oil drops is enhanced when covered by insulating polyethylene (PE) particles, whereas drops covered by conductive clay particles can also change shape from oblate to prolate. We attribute these results to changes in the electric conductivity of the drop interface after adding particles, and have developed a fluid shell description to estimate the conductivity of Pickering particle layers that are assumed to be non-jammed and fluid-like. Retraction experiments in the absence of electric fields are also performed. Particle-covered drops retract slower than particle-free drops, caused by increased viscous dissipation due to the presence of the Pickering particle layer.
Antibacterial and Antifungal Activity of Essential Oils against Pathogens Responsible for Otitis Externa in Dogs and Cats
Authors: Ebani Valentina , Nardoni Simona , Bertelloni Fabrizio , Najar Basma , Pistelli Luisa , Mancianti Francesca
Published: April 2017

Background: Essential oils (EOs) are recommended by some veterinarians to treat otitis externa in pets, but data about their efficacy in scientific literature are very scant. Methods: Nine commercial EOs, from roman chamomile (Anthemis nobilis L.), star anise (Illicium verum), lavender (Lavandula hybrida), litsea (Litsea cubeba (Lour.) Pers.), basil (Ocimum basilicum L.), oregano (Origanum vulgare L. subsp. hirticum), rosemary (Rosmarinus officinalis L.), clary sage (Salvia sclarea L.), and thyme (Thymus vulgaris L.) were tested against bacterial and fungal pathogens previously isolated from dogs and cats with otitis externa. In particular, the analyses were carried out against Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus pseudointermedius, Aspergillus niger, Aspergillus fumigatus, Aspergillus terreus, Candida albicans, Candida tropicalis, Trichosporon sp., and Rhodotorula sp. Results: O. vulgare and S. sclarea showed superior antibacterial activity, even if not against all the strains. Trichosporon sp., C. albicans, and A. terreus were insensitive to most Eos, while other yeasts and molds showed different degrees of sensitivity. In particular, most fungi were inhibited by O. vulgare and R. officinalis. Conclusions: The obtained results suggest that some EOs could be included in treatment as an alternative therapeutic option in bacterial otitis complicated by fungi, in association with conventional drugs.
Efficient Use of Porous Hybrid Materials in a Selective Detection of Lead(II) from Aqueous Solutions: An Electrochemical Study
Authors: Zirlianngura  , Tiwari Diwakar , Ha Jeong-Hyub , Lee Seung-Mok
Published: April 2017

Due to health and pollution concerns of aquatic environments related to the presence of heavy metal toxic ions, the necessity of developing devices able to detect and to monitor such kinds of species has recently gained importance. Carbon paste electrodes (CPEs) a starting approach to obtain new ion-selective devices by supporting materials like bentonite and/or clay; which become sensitive to lead(II) when they are suitably modified by chemical treatments to obtain different hybrid materials. In this work, two natural clays and three different hybrid materials were produced and then were characterized by X-ray diffraction, scanning electron microscopy, and Fourier transform infrared spectroscopy to assess their physico-chemical properties. After this stage, the electrochemical characterization of the modified CPEs using hybrid materials was performed by cyclic voltammetry, using the standard Fe(CN)64−/Fe(CN)63− redox couple. Subsequently, this study performed electrochemical experiments on lead(II) containing solutions, to test the ability of the examined CPEs to detect this toxic ion present in very low amounts. Lead(II) exhibited a reversible two electron oxidation/reduction behaviour in the cyclic voltammetry analyses and a reasonably good linear behaviour of the current associated with the oxidation peak as a function of its concentration (5.0–40.0 μg/L). The detection limit was found to vary in the range of 3–5 μg/L for the different modified CPEs. The presence of several co-existing ions showed that an interference variation had occurred. These results, therefore, show a restriction of the selectivity of the electrode up to a certain extent in the lead(II) detection. Finally, tap water with spiked lead(II) was analyzed to verify the suitability of the electrodes in the low level detection of lead(II) from real matrix samples.
Gamma-Aminobutyric Acid Increases the Production of Short-Chain Fatty Acids and Decreases pH Values in Mouse Colon
Authors: Xie Min , Chen Hai-Hong , Nie Shao-Ping , Yin Jun-Yi , Xie Ming-Yong
Published: April 2017

Gamma-Aminobutyric acid (GABA) could regulate physiological functions in the gastrointestinal tract. The present study aimed to investigate the effect of GABA on colon health in mice. The female Kunming mice were given GABA at doses of 5, 10, 20 and 40 mg/kg/d for 14 days. Afterwards, the short-chain fatty acids (SCFAs) concentrations, pH values, colon index, colon length and weight of colonic and cecal contents were determined to evaluate the effects of GABA on colon health. The results showed that intake of GABA could increase the concentrations of acetate, propionate, butyrate and total SCFAs in colonic and cecal contents, as well as the weight of colonic and cecal contents. The colon index and length of the 40 mg/kg/d GABA-treated group were significantly higher than those of the control group (p < 0.05). In addition, decrease of pH values in colonic and cecal contents was also observed. These results suggest that GABA may improve colon health.
Evaluation of Tanshinone IIA Developmental Toxicity in Zebrafish Embryos
Authors: Wang Tao , Wang Chengxi , Wu Qiong , Zheng Kangdi , Chen Jiaojiao , Lan Yutao , Qin Yao , Mei Wenjie , Wang Baoguo
Published: April 2017

Tanshinone IIA (Tan-IIA) is derived from the dried roots of Salvia miltiorrhiza Bunge, a traditional Chinese medicine. Although Salvia miltiorrhiza has been applied for many years, the toxicity of the mono-constituent of Salvia miltiorrhiza, tanshinone IIA, is still understudied. This study evaluated the cardiotoxicity and developmental malformations of Tan-IIA by using zebrafish normal embryos and dechorionated embryos. After treatment with Tan-IIA in different concentrations for four-day periods, obvious pericardial edema, spinal curvature, and even missing tails were observed in zebrafish embryos. The LC50 values in the dechorionated embryo group at 72 h post-fertilization (hpf) and 96 hpf were 18.5 μM and 12.8 μM, respectively, and the teratogenicity was manifested at a concentration of about 1 µM. The main endpoints of teratogenicity were scoliosis, malformation of tail, and pericardium edema. Our findings displayed the potential cardiotoxicity and severe impact on the abnormal development of Tan-IIA in zebrafish embryo at high concentrations, which may help avoid the risk of its clinical application.
Reactions of 5-Indolizyl Lithium Compounds with Some Bielectrophiles
Authors: Rzhevskii Sergey A. , Rybakov Victor B. , Khrustalev Victor N. , Babaev Eugene V.
Published: April 2017

Abstract: Indolizyl-5-lithium anions react with succinic and phtalic anhidrides giving 1,4-keto acids, with oxallyl chloride giving 1,2-diketone, and with ethyl pyruvate giving 1,2-hydroxyacid. However, with α-halocarbonyl compounds, they react in different ways, forming the products of selective bromination at C-5 (with α-bromo ketones and esters of α-bromo acids) and 5-chloroacetyl indolizines.
Ameliorative Effects and Possible Molecular Mechanism of Action of Black Ginseng (Panax ginseng) on Acetaminophen-Mediated Liver Injury
Authors: Hu Jun-Nan , Liu Zhi , Wang Zi , Li Xin-Dian , Zhang Lian-Xue , Li Wei , Wang Ying-Ping
Published: April 2017

Background: Frequent overdosing of acetaminophen (APAP) has become the major cause of acute liver injury (ALI). The present study aimed to evaluate the potential hepatoprotective effects of black ginseng (BG) on APAP-induced mice liver injuries and the underlying mechanisms of action were further investigated for the first time. Methods: Mice were treated with BG (300, 600 mg/kg) by oral gavage once a day for seven days. On the 7th day, all mice were treated with 250 mg/kg APAP which caused severe liver injury after 24 h and hepatotoxicity was assessed. Results: Our results showed that pretreatment with BG significantly decreased the levels of serum alanine aminotransferase (ALT) and aspartate transaminase (AST) compared with the APAP group. Meanwhile, hepatic antioxidant including glutathione (GSH) was elevated compared with the APAP group. In contrast, a significant decrease of the levels of the lipid peroxidation product malondialdehyde (MDA) was observed in the BG-treated groups compared with the APAP group. These effects were associated with significant increases of cytochrome P450 E1 (CYP2E1) and 4-hydroxynonenal (4-HNE) levels in liver tissues. Moreover, BG supplementation suppressed activation of apoptotic pathways through increasing Bcl-2 and decreasing Bax protein expression levels according to western blotting analysis. Histopathological examination revealed that BG pretreatment significantly inhibited APAP-induced necrosis and inflammatory infiltration in liver tissues. Biological indicators of nitrative stress like 3-nitrotyrosine (3-NT) were also inhibited after pretreatment with BG, compared with the APAP group. Conclusions: The results clearly suggest that the underlying molecular mechanisms of action of BG-mediated alleviation of APAP-induced hepatotoxicity may involve its anti-oxidant, anti-apoptotic, anti-inflammatory and anti-nitrative effects.
Chocolate Consumption and Risk of Heart Failure: A Meta-Analysis of Prospective Studies
Authors: Gong Fei , Yao Shuyuan , Wan Jing , Gan Xuedong
Published: April 2017

Epidemiological studies have shown inconsistent findings on the association between chocolate consumption and risk of heart failure (HF). We, therefore, performed a meta-analysis of prospective studies to determine the role of chocolate intake in the prevention of HF. We searched databases of PubMed, Web of Science, and Scopus through December 2016 and scrutinized the reference lists of relevant literatures to identify eligible studies. Study-specific hazard ratios (HRs) and 95% confidence intervals (CIs) were aggregated using random effect models. The dose–response relationship between chocolate consumption and incident HF was also assessed. This meta-analysis is registered with PROSPERO, number CRD42017054230. Five prospective studies with 106,109 participants were finally included. Compared to no consumption of chocolate, the pooled HRs (95% CIs) of HF were 0.86 (0.82–0.91) for low-to-moderate consumption (<7 servings/week) and 0.94 (0.80–1.09) for high consumption (≥7 servings/week). In dose–response meta-analysis, we detected a curve linear relationship between chocolate consumption and risk of HF (p for nonlinearity = 0.005). Compared with non-consumption, the HRs (95% CIs) of HF across chocolate consumption levels were 0.92 (0.88–0.97), 0.86 (0.78–0.94), 0.93 (0.85–1.03), and 1.07 (0.92–1.23) for 1, 3, 7, and 10 servings/week, respectively. In conclusion, chocolate consumption in moderation may be associated with a decreased risk of HF.
Genistein Ameliorates Ischemia/Reperfusion-Induced Renal Injury in a SIRT1-Dependent Manner
Authors: Li Wei-Fang , Yang Kang , Zhu Ping , Zhao Hong-Qian , Song Yin-Hong , Liu Kuan-Can , Huang Wei-Feng
Published: April 2017

Renal ischemia/reperfusion (I/R) injury continues to be a complicated situation in clinical practice. Genistein, the main isoflavone found in soy products, is known to possess a wide spectrum of biochemical and pharmacological activities. However, the protective effect of genistein on renal I/R injury has not been well investigated. In the current study, we explore whether genistein exhibits its renal-protective effects through SIRT1 (Sirtuin 1) in I/R-induced mice model. We found the treatment of genistein significantly reduced renal I/R-induced cell death, simultaneously stimulating renal cell proliferation. Meanwhile, SIRT1 expression was up-regulated following the administration of genistein in renal region. Furthermore, pharmacological inhibition or shRNA-mediated depletion of SIRT1 significantly reversed the protective effect of genistein on renal dysfunction, cellular damage, apoptosis, and proliferation following I/R injury, suggesting an indispensible role of the increased SIRT1 expression and activity in this process. Meanwhile, the reduced p53 and p21 expression and increased PCNA (Proliferating Cell Nuclear Antigen) expression were blocked after the depletion of SIRT1 compared with the genistein treatment group in the renal I/R process. Hence, our results provided further experimental basis for the potential use of genistein for the treatment of kidney disease with deficiency of SIRT1 activity.
Testing the Capacity of a Multi-Nutrient Profiling System to Guide Food and Beverage Reformulation: Results from Five National Food Composition Databases
Authors: Combet Emilie , Vlassopoulos Antonis , Mölenberg Famke , Gressier Mathilde , Privet Lisa , Wratten Craig , Sharif Sahar , Vieux Florent , Lehmann Undine , Masset Gabriel
Published: April 2017

Nutrient profiling ranks foods based on their nutrient composition, with applications in multiple aspects of food policy. We tested the capacity of a category-specific model developed for product reformulation to improve the average nutrient content of foods, using five national food composition datasets (UK, US, China, Brazil, France). Products (n = 7183) were split into 35 categories based on the Nestlé Nutritional Profiling Systems (NNPS) and were then classified as NNPS ‘Pass’ if all nutrient targets were met (energy (E), total fat (TF), saturated fat (SFA), sodium (Na), added sugars (AS), protein, calcium). In a modelling scenario, all NNPS Fail products were ‘reformulated’ to meet NNPS standards. Overall, a third (36%) of all products achieved the NNPS standard/pass (inter-country and inter-category range: 32%–40%; 5%–72%, respectively), with most products requiring reformulation in two or more nutrients. The most common nutrients to require reformulation were SFA (22%–44%) and TF (23%–42%). Modelled compliance with NNPS standards could reduce the average content of SFA, Na and AS (10%, 8% and 6%, respectively) at the food supply level. Despite the good potential to stimulate reformulation across the five countries, the study highlights the need for better data quality and granularity of food composition databases.
Endurance Training with or without Glucose-Fructose Ingestion: Effects on Lactate Metabolism Assessed in a Randomized Clinical Trial on Sedentary Men
Authors: Rosset Robin , Lecoultre Virgile , Egli Léonie , Cros Jérémy , Rey Valentine , Stefanoni Nathalie , Sauvinet Valérie , Laville Martine , Schneiter Philippe , Tappy Luc
Published: April 2017

Glucose-fructose ingestion increases glucose and lactate oxidation during exercise. We hypothesized that training with glucose-fructose would induce key adaptations in lactate metabolism. Two groups of eight sedentary males were endurance-trained for three weeks while ingesting either glucose-fructose (GF) or water (C). Effects of glucose-fructose on lactate appearance, oxidation, and clearance were measured at rest and during exercise, pre-training, and post-training. Pre-training, resting lactate appearance was 3.6 ± 0.5 vs. 3.6 ± 0.4 mg·kg−1·min−1 in GF and C, and was increased to 11.2 ± 1.4 vs. 8.8 ± 0.7 mg·kg−1·min−1 by exercise (Exercise: p < 0.01). Lactate oxidation represented 20.6% ± 1.0% and 17.5% ± 1.7% of lactate appearance at rest, and 86.3% ± 3.8% and 86.8% ± 6.6% during exercise (Exercise: p < 0.01) in GF and C, respectively. Training with GF increased resting lactate appearance and oxidation (Training × Intervention: both p < 0.05), but not during exercise (Training × Intervention: both p > 0.05). Training with GF and C had similar effects to increase lactate clearance during exercise (+15.5 ± 9.2 and +10.1 ± 5.9 mL·kg−1·min−1; Training: p < 0.01; Training × Intervention: p = 0.97). The findings of this study show that in sedentary participants, glucose-fructose ingestion leads to high systemic lactate appearance, most of which is disposed non-oxidatively at rest and is oxidized during exercise. Training with or without glucose-fructose increases lactate clearance, without altering lactate appearance and oxidation during exercise.
The Association of Ankle Brachial Index, Protein-Energy Wasting, and Inflammation Status with Cardiovascular Mortality in Patients on Chronic Hemodialysis
Authors: Ishii Hideki , Takahashi Hiroshi , Ito Yasuhiko , Aoyama Toru , Kamoi Daisuke , Sakakibara Takashi , Umemoto Norio , Kumada Yoshitaka , Suzuki Susumu , Murohara Toyoaki
Published: April 2017

Protein-energy wasting (PEW) is highly prevalent in hemodialysis (HD) patients. We investigated the association of abnormal ankle brachial index (ABI), PEW, and chronic inflammation status with clinical prognosis in HD patients. A total of 973 HD patients were enrolled and were followed-up for 8 years. As a marker of the PEW, geriatric nutritional risk index (GNRI) was used. Cut-off levels were 91.2 for GNRI defined from previous studies and 1.9 mg/L for C-reactive protein (CRP) as median value, respectively. Abnormal ABI was seen in 332 (34.1%) patients. Declined GNRI and elevated CRP levels were independently associated with abnormal ABI (odds ratio (OR) 0.97, 95% confidence interval (CI) 0.96–0.99, p = 0.0009 and OR 1.40, 95% CI 1.07–1.83, p = 0.013, respectively). GNRI levels were also independently correlated with CRP levels (β = −0.126, p < 0.0001). During follow-up period, 283 (29.1%) patients died, including 123 (12.6%) due to cardiovascular disease (CVD). Abnormal ABI (adjusted hazard ratio (HR) 1.62, 95% CI 1.13–2.32, p = 0.0096), GNRI < 91.2 (adjusted HR 1.57, 95% CI 1.06–2.33, p = 0.023) and CRP > 1.9 mg/L (adjusted HR 1.89, 95% CI 1.31–2.77, p = 0.0007) independently predicted mortality due to CVD, respectively. In conclusion, abnormal ABI, GNRI, and CRP levels were closely associated with each other, and the combination of these variables increase their predictive values for the risk of mortality due to CVD and all-cause mortality in HD patients.
Morphology Control of Ni(II)-NTA-End-Functionalized Block Copolymer and Bio-Conjugation through Metal-Ligand Complex
Authors: Park Dasom , Lee Chaeyeon , Chae Minsu , Kadir Mohammad , Choi Ji , Song Jae , Paik Hyun-jong
Published: April 2017

This study demonstrates the synthesis of an amphiphilic block copolymer, Ni2+-nitrilotiracetic acid-end-functionalized-poly(poly(ethylene glycol)methyl ether methacrylate)-block-polystyrene (NTA-p(PEGMA-b-St)), morphology control via their self-assembly behavior and reversible bioconjugation of hexahistidine-tagged green fluorescent protein (His6-GFP) onto the surfaces of polymeric vesicles through nitrilotriacetic acid (NTA)-Ni2+-His interaction. First, the t-boc-protected-NTA-p(PEGMA-b-St) was synthesized by atom transfer radical polymerization. After the removal of the t-boc protecting group, the NTA group of the polymer was complexed with Ni2+. To induce self-assembly, water was added as a selective solvent to the solution of the copolymer in tetrahydrofuran (THF). Varying the water content of the solution resulted in various morphologies including spheres, lamellas and vesicles. Finally, polymeric vesicles decorated with green fluorescent protein (GFP) on their surfaces were prepared by the addition of His6-GFP into the vesicles solution. Reversibility of the binding between vesicles and His6-GFP was confirmed with a fluorescent microscope.
Biodegradable and Biocompatible PDLLA-PEG1k-PDLLA Diacrylate Macromers: Synthesis, Characterisation and Preparation of Soluble Hyperbranched Polymers and Crosslinked Hydrogels
Authors: Hughes Alan , Tai Hongyun , Tochwin Anna , Wang Wenxin
Published: April 2017

A series of PDLLA-PEG1k-PDLLA tri-block co-polymers with various compositions, i.e., containing 2–10 lactoyl units, were prepared via ring opening polymerisation of d,l-lactide in the presence of poly (ethylene glycol) (PEG) (Mn = 1000 g·mol−1) as the initiator and stannous 2-ethylhexanoate as the catalyst at different feed ratios. PDLLA-PEG1k-PDLLA co-polymers were then functionalised with acrylate groups using acryloyl chloride under various reaction conditions. The diacrylated PDLLA-PEG1k-PDLLA (diacryl-PDLLA-PEG1k-PDLLA) were further polymerised to synthesize soluble hyperbranched polymers by either homo-polymerisation or co-polymerisation with poly(ethylene glycol) methyl ether methylacrylate (PEGMEMA) via free radical polymerisation. The polymer samples obtained were characterised by 1H NMR (proton Nuclear Magnetic Resonance), FTIR (Fourier Transform Infra-red spectroscopy), and GPC (Gel Permeation Chromatography). Moreover, the diacryl-PDLLA-PEG1k-PDLLA macromers were used for the preparation of biodegradable crosslinked hydrogels through the Michael addition reaction and radical photo-polymerisation with or without poly(ethylene glycol) methyl ether methylacrylate (PEGMEMA, Mn = 475 g·mol−1) as the co-monomer. It was found that fine tuning of the diacryl-PDLLA-PEG1k-PDLLA constituents and its combination with co-monomers resulted in hydrogels with tailored swelling properties. It is envisioned that soluble hyperbranched polymers and crosslinked hydrogels prepared from diacryl-PDLLA-PEG1k-PDLLA macromers can have promising applications in the fields of nano-medicines and regenerative medicines.
Kinetics of the Aqueous-Phase Copolymerization of MAA and PEGMA Macromonomer: Influence of Monomer Concentration and Side Chain Length of PEGMA
Authors: Emaldi Iñaki , Hamzehlou Shaghayegh , Sanchez-Dolado Jorge , Leiza Jose
Published: April 2017

An in situ nuclear magnetic resonance spectroscopy (NMR) technique is used to monitor the aqueous-phase copolymerization kinetics of methacrylic acid (MAA) and poly(ethylene glycol) methyl ether methacrylate (PEGMA) macromonomers. In particular, the study analyses the effect of the number of ethylene glycol (EG) groups along the lateral chains of PEGMA and is carried out under fully ionized conditions of MAA at different initial monomer ratios and initial overall monomer concentrations (5–20 wt % in aqueous solution). The composition drift with conversion indicates that PEGMA macromonomer is more reactive than MAA. Individual monomer consumption rates show that the rates of consumption of both monomers are not first order with respect to overall concentration of the monomer. The reactivity ratios estimated from the copolymerization kinetics reveal, that for the short PEGMA, the reactivity ratios rMAA and rPEGMA increase with the solids content (SC). A totally different trend is obtained for the longer PEGMA, whose reactivity ratio (rPEGMA23) decreases with solids content, whereas the reactivity ratio of MAA remains roughly constant.
The Protestant Reformers and the Jews: Excavating Contexts, Unearthing Logic
Authors: Pak G.
Published: April 2017

This article highlights the important initial tasks of excavating the pertinent contexts of the sixteenth-century Protestant reformers and discerning what is at stake for them (i.e., “unearthing logic”) in order to analyze their views of and teachings about Jews and Judaism. Pertinent contexts include the immediate contexts to which Luther and Calvin responded (e.g., Jewish “blasphemy” and/or Christian Hebraism), as well as attending to the significant theological frameworks in which they each operated. Equally important is activity of sifting through the discrepancies in the secondary literature’s depictions of Luther and Calvin’s place in the history of Christian-Jewish relations. The article highlights biblical interpretation—particularly the defense of Scripture’s perspicuity—as the distinctive locus of the reformers’ angst concerning Jews and Judaism. In conclusion, the author offers some lessons from church history for discerning what Christian faithfulness might look like in response to this troubling history.
The Economic and Ethical Implications of Living Wages
Authors: Konigsburg Joyce
Published: April 2017

Although rhetoric about wages and jobs often emphasizes the effects of globalization, questions remain as to whether United States workers are paid adequately to sustain a reasonable standard of living. One solution is to implement a living wage, which is accurate and specific to a local economy but more computationally complex than a one-size-fits-all minimum wage. When considered economically, a living wage has the potential to increase business and production costs as well as lower profits and cause job loss. From ethical viewpoints articulated in Catholic social thought, sustainable wages enhance human dignity by supporting human agency, encouraging creativity, and permitting contributions to the common good. This article explores whether the positive ethical outcomes of implementing a living wage outweigh any possibly negative, unintended economic results.
A Glacier Surge of Bivachny Glacier, Pamir Mountains, Observed by a Time Series of High-Resolution Digital Elevation Models and Glacier Velocities
Authors: Wendt Anja , Mayer Christoph , Lambrecht Astrid , Floricioiu Dana
Published: April 2017

Surge-type glaciers are characterised by relatively short phases of enhanced ice transport and mass redistribution after a comparatively long quiescent phase when the glacier is virtually inactive. This unstable behaviour makes it difficult to assess the influence of climate change on those glaciers. We describe the evolution of the most recent surge of Bivachny Glacier in the Pamir Mountains, Tajikistan between 2011 and 2015 with respect to changes in its topography and dynamics. For the relevant time span, nine digital elevation models were derived from TanDEM-X data; optical satellite data (Landsat 5, 7 and 8, EO-1) as well as synthetic aperture radar data (TerraSAR-X and TanDEM-X) were used to analyse ice flow velocities. The comparison of the topography at the beginning of the surge with the one observed by the Shuttle Radar Topography Mission in 2000 revealed a thickening in the upper part of the ablation area of the glacier and a thinning further down the glacier as is typically observed during the quiescent phase. During the active phase, a surge bulge measuring up to around 80 m developed and travelled downstream for a distance of 13 km with a mean velocity of 4400 m year−1. Ice flow velocities increased from below 90 m year−1 duringthe quiescent phase in 2000 to up to 3400 m year−1 in spring 2014. After reaching the confluence with Fedchenko Glacier, the surge slowed down until it completely terminated in 2015. The observed seasonality of the glacier velocities with a regular speed-up during the onset of the melt period suggests a hydrological control of the surge related to the effectiveness of the subglacial drainage system.
Deep Count: Fruit Counting Based on Deep Simulated Learning
Authors: Rahnemoonfar Maryam , Sheppard Clay
Published: April 2017

Recent years have witnessed significant advancement in computer vision research based on deep learning. Success of these tasks largely depends on the availability of a large amount of training samples. Labeling the training samples is an expensive process. In this paper, we present a simulated deep convolutional neural network for yield estimation. Knowing the exact number of fruits, flowers, and trees helps farmers to make better decisions on cultivation practices, plant disease prevention, and the size of harvest labor force. The current practice of yield estimation based on the manual counting of fruits or flowers by workers is a very time consuming and expensive process and it is not practical for big fields. Automatic yield estimation based on robotic agriculture provides a viable solution in this regard. Our network is trained entirely on synthetic data and tested on real data. To capture features on multiple scales, we used a modified version of the Inception-ResNet architecture. Our algorithm counts efficiently even if fruits are under shadow, occluded by foliage, branches, or if there is some degree of overlap amongst fruits. Experimental results show a 91% average test accuracy on real images and 93% on synthetic images.
Dynamic Construction Scheme for Virtualization Security Service in Software-Defined Networks
Authors: Lin Zhaowen , Tao Dan , Wang Zhenji
Published: April 2017

For a Software Defined Network (SDN), security is an important factor affecting its large-scale deployment. The existing security solutions for SDN mainly focus on the controller itself, which has to handle all the security protection tasks by using the programmability of the network. This will undoubtedly involve a heavy burden for the controller. More devastatingly, once the controller itself is attacked, the entire network will be paralyzed. Motivated by this, this paper proposes a novel security protection architecture for SDN. We design a security service orchestration center in the control plane of SDN, and this center physically decouples from the SDN controller and constructs SDN security services. We adopt virtualization technology to construct a security meta-function library, and propose a dynamic security service composition construction algorithm based on web service composition technology. The rule-combining method is used to combine security meta-functions to construct security services which meet the requirements of users. Moreover, the RETE algorithm is introduced to improve the efficiency of the rule-combining method. We evaluate our solutions in a realistic scenario based on OpenStack. Substantial experimental results demonstrate the effectiveness of our solutions that contribute to achieve the effective security protection with a small burden of the SDN controller.
A Multicriteria Model to Evaluate Strategic Plans for the Nautical and Naval Industry in Cartagena de Indias, Colombia
Authors: Gonzalez-Urango Hannia , García-Melón Mónica
Published: April 2017

The evaluation of urban development plans is a key concern of the strategic planning of the city of Cartagena de Indias (Colombia) due to the pressure exerted by both public and private sectors. Any strategic planning requirement deserves the inclusion of clear terms of coordination and cooperation among sectors, including local communities and the scientific sector. In this paper, we present a methodology for the sustainable evaluation of strategic nautical and naval projects for the development of the city of Cartagena de Indias. The methodology is based on the multicriteria technique Analytic Network Process, which allows considering political, socio-cultural and environmental aspects. The aim is to provide answers and guide the decision makers towards the optimal selection of strategies. Results provide some important insights into the overall conception of what sustainable evaluation means for the experts consulted. The procedure enhances participation and transparency and becomes a support for their decisions.