bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Potential Inhibitors of Histone Deacetylase 6 Through Virtual Screening and Molecular Dynamics Simulation Approach: Implications in Neurodegenerative Diseases
Authors: Shamsi Anas , Shahwan Moyad , Zuberi Azna , Altwaijry Nojood
Published: November 2024

Background: Histone deacetylase 6 (HDAC6) plays a crucial role in neurological, inflammatory, and other diseases; thus, it has emerged as an important target for therapeutic intervention. To date, there are no FDA-approved HDAC6-targeting drugs, and most pipeline candidates suffer from poor target engagement, inadequate brain penetration, and low tolerability. There are a few HDAC6 clinical candidates for the treatment of mostly non-CNS cancers as their pharmacokinetic liabilities exclude them from targeting HDAC6-implicated neurological diseases, urging development to address these challenges. They also demonstrate off-target toxicity due to limited selectivity, leading to adverse effects in patients. Selective inhibitors have thus been the focus of development over the past decade, though no selective and potent HDAC6 inhibitor has yet been approved. Methods: This study involved an integrated virtual screening against HDAC6 using the DrugBank database to identify repurposed drugs capable of inhibiting HDAC6 activity. The primary assessment involved the determination of the ability of molecules to bind with HDAC6. Subsequently, interaction analyses and 500 ns molecular dynamics (MD) simulations followed by essential dynamics were carried out to study the conformational flexibility and stability of HDAC6 in the presence of the screened molecules, i.e., penfluridol and pimozide. Results: The virtual screening results pinpointed penfluridol and pimozide as potential repurposed drugs against HDAC6 based on their binding efficiency and appropriate drug profiles. The docking results indicate that penfluridol and pimozide share the same binding site as the reference inhibitor with HDAC6. The MD simulation results showed that stable protein–ligand complexes of penfluridol and pimozide with HDAC6 were formed. Additionally, MMPBSA analysis revealed favorable binding free energies for all HDAC6–ligand complexes, confirming the stability of their interactions. Conclusions: The study implies that both penfluridol and pimozide have strong and favorable binding with HDAC6, which supports the idea of repositioning these drugs for the management of neurodegenerative disorders. However, further in-depth studies are needed to explore their efficacy and safety in biological systems.
Antibacterial Mechanisms and Clinical Impact of Sitafloxacin
Authors: Kuhn Elian M. A. , Sominsky Levy A. , Chittò Marco , Schwarz Edward M. , Moriarty T. Fintan
Published: November 2024

Sitafloxacin is a 4th generation fluoroquinolone antibiotic with broad activity against a wide range of Gram-negative and Gram-positive bacteria. It is approved in Japan and used to treat pneumonia and urinary tract infections (UTIs) as well as other upper and lower respiratory infections, genitourinary infections, oral infections and otitis media. Compared to other fluoroquinolones, sitafloxacin displays a low minimal inhibitory concentration (MIC) for many bacterial species but also activity against anaerobes, intracellular bacteria, and persisters. Furthermore, it has also shown strong activity against biofilms of P. aeruginosa and S. aureus in vitro, which was recently validated in vivo with murine models of S. aureus implant-associated bone infection. Although limited in scale at present, the published literature supports the further evaluation of sitafloxacin in implant-related infections and other biofilm-related infections. The aim of this review is to summarize the chemical-positioning-based mechanisms, activity, resistance profile, and future clinical potential of sitafloxacin.
Effects of Short-Term Treatment with α-Lipoic Acid on Neuropathic Pain and Biomarkers of DNA Damage in Patients with Diabetes Mellitus
Authors: Lazutka Juozas R. , Daniūnaitė Kristina , Dedonytė Veronika , Popandopula Aistė , Žukaitė Karolina , Visockienė Žydrūnė , Šiaulienė Laura
Published: November 2024

Background/Objectives: Diabetes mellitus (DM) is a complex and heterogenous disease classified as a group of metabolic disorders characterized by chronic hyperglycemia resulting from defects in insulin secretion, insulin action, or both. It leads to various complications, some of which are macrovascular or microvascular complications, like diabetic polyneuropathy (DPN), having a profound impact on patients’ quality of life. Oxidative stress (OS) is one of the significant mechanisms in the development and progression of DPN. Thus, targeting OS pathways by antioxidants, such as α-lipoic acid (ALA), could represent a promising therapeutic strategy for alleviating neuropathic symptoms. The aim of our study was to evaluate whether short-term (from 4 to 9 days) intravenous administration of ALA could cause any measurable improvement in subjects with DM. Methods: Sixteen subjects with DM (six type 1 and ten type 2) and sixteen nondiabetic subjects matched by sex and age were recruited to this study. Only subjects with DM received treatment with ALA (600 mg daily). Pain intensity and biomarkers of DNA damage including plasma concentration of 8-hydroxy-2′-deoxyguanosine (8-OHdG), frequency of micronucleated lymphocytes (MN), and frequency of sister-chromatid exchanges (SCEs), were measured before and after the treatment with ALA. Results: Pain intensity and 8-OHdG levels were significantly lower in DM subjects after the ALA treatment than before the treatment. However, no changes in the frequency of SCEs and MN were observed. Conclusions: Our results show some evidence that even a short-term intravenous treatment with ALA could be beneficial for diabetic subjects, reducing pain intensity and concentration of 8-OHdG in blood plasma.
Immobilization of Silver Nanoparticles with Defensive Gum of Moringa oleifera for Antibacterial Efficacy Against Resistant Bacterial Species from Human Infections
Authors: Ali Liaqat , Ahmad Nisar , Uddin Muhammad Nazir , Abdel-Maksoud Mostafa A. , Fazal Hina , Fatima Sabiha , El-Tayeb Mohamed A. , Kiani Bushra Hafeez , Khan Wajid , Rahat Murad Ali , Ali Mohammad , Khan Yaqub , Rauf Kamran , Khan Salman , Ullah Sami , Ahmad Tanveer , Salam Afshan , Ahmad Sajjad
Published: November 2024

Background: The worldwide misuse of antibiotics is one of the main factors in microbial resistance that is a serious threat worldwide. Alternative strategies are needed to overcome this issue. Objectives: In this study, a novel strategy was adopted to suppress the growth of resistant pathogens through immobilization of silver nanoparticles (AgNPs) in gum of Moringa oleifera. Methods: The AgNPs were prepared from the leaves of Moringa oleifera and subsequently characterized through UV-spectrophotometry, FTIR, SEM, and XRD. The differential ratios of characterized AgNPs were immobilized with gum of M. oleifera and investigated for antimicrobial potential against highly resistant pathogens. Results: The immobilized AgNPs displayed promising activities against highly resistant B. subtilis (23.6 mm; 50 µL:200 µL), E. coli (19.3 mm; 75 µL:200 µL), K. pneumoniae (22 mm; 200 µL:200 µL), P. mirabilis (16.3 mm; 100 µL:200 µL), P. aeruginosa (22 mm; 175 µL:200 µL), and S. typhi (19.3; 25 µL:200 µL) than either AgNPs alone or gum. The immobilized AgNPs released positive sliver ions that easily attached to negatively charged bacterial cells. After attachment and permeation to bacterial cells, the immobilized NPs alter the cell membrane permeability, protein/enzymes denaturation, oxidative stress (ROS), damage DNA, and change the gene expression level. It has been mechanistically considered that the immobilized AgNPs can kill bacteria by damaging their cell membranes, dephosphorylating tyrosine residues during their signal transduction pathways, inducing cell apoptosis, rupturing organelles, and inhibiting cell division, which finally leads to cell death. Conclusions: This study proposes a potential alternative drug for curing various infections.
A Comprehensive Review and Update on Cannabis Hyperemesis Syndrome
Authors: Loganathan Priyadarshini , Gajendran Mahesh , Goyal Hemant
Published: November 2024

Cannabis, derived from Cannabis sativa plants, is a prevalent illicit substance in the United States, containing over 400 chemicals, including 100 cannabinoids, each affecting the body’s organs differently upon ingestion. Cannabis hyperemesis syndrome (CHS) is a gut–brain axis disorder characterized by recurring nausea and vomiting intensified by excessive cannabis consumption. CHS often goes undiagnosed due to inconsistent criteria, subjective symptoms, and similarity to cyclical vomiting syndrome (CVS). Understanding the endocannabinoid system (ECS) and its dual response (pro-emetic at higher doses and anti-emetic at lower doses) is crucial in the pathophysiology of CHS. Recent research noted that type 1 cannabinoid receptors in the intestinal nerve plexus exhibit an inhibitory effect on gastrointestinal motility. At the same time, the thermoregulatory function of endocannabinoids might explain compulsive hot bathing in CHS patients. The prevalence of cannabis CHS is expected to rise as legal restrictions on its recreational use decrease in several states. Education and awareness are vital in diagnosing and treating CHS as its prevalence increases. This comprehensive review explores the ECS’s involvement, CHS management approaches, and knowledge gaps to enhance understanding of this syndrome.
When Light Is Crucial, but Wind Is Rather Trivial: A Basil Case Study
Authors: Florou Efterpi , Politi Angela , Andreadaki Evangelia , Vrakas Konstantinos , Spaliara Hariklia , Neli Alexandros , Koulopoulou Christina Eleni , Koulopoulos Athanasios , Bantis Filippos , Zervoudakis George
Published: November 2024

Light intensity and wind are critical environmental factors of abiotic stress on plants, triggering a considerable number of morphological and physiological responses. This study tested the hypothesis that different light and wind conditions (full sunlight ± wind, shade ± wind) would affect the leaf content of photosynthetic pigments and anthocyanins, as well as the plant height, the fresh and dry weight of the aboveground part, and Water-Use Efficiency on Ocimum basilicum plants. About 16 days after the application of the different conditions, all leaf pigments of the shaded plants exhibited increased contents compared to the lightened ones. Subsequently, this response was enhanced until the 39th day, which was the final day of the experiment. Furthermore, shaded plants grew taller, although their fresh and dry weight and Water-Use Efficiency were lower than that of the corresponding lightened ones. On the other hand, wind did not have any effect on pigment content. Concerning the plant growth indexes, reduced values were observed on the wind-treated plants. The above results demonstrate a considerable effect of light intensity while the applied wind seems to be mild enough to induce important plant responses, partially confirming the hypothesis studied.
Establishment of Betalain-Producing Cell Line and Optimization of Pigment Production in Cell Suspension Cultures of Celosia argentea var. plumosa
Authors: Sang A Roon Thapagorn , Klanrit Poramaporn , Klanrit Poramate , Thanonkeo Pornthap , Apiraksakorn Jirawan , Thanonkeo Sudarat , Klanrit Preekamol
Published: November 2024

The prevalence of synthetic colorants in commercial products has raised concerns regarding potential risks, including allergic reactions and carcinogenesis, associated with their use or consumption. Natural plant extracts have gained attention as potential alternatives. This research focuses on callus induction and the establishment of cell suspension cultures from Celosia argentea var. plumosa. Friable callus was successfully induced using hypocotyl explants cultured on semi-solid Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1 mg/L 6-benzylaminopurine (BAP). The friable callus cell line was used to establish a suspension culture. The effects of sucrose, BAP, and tyrosine concentrations on betalain production were investigated using response surface methodology (RSM) based on central composite design (CCD). Optimal conditions (43.88 g/L sucrose, 0.15 mg/L tyrosine, and 0.77 mg/L BAP) yielded 43.87 mg/L total betalain content after 21 days, representing a threefold increase compared to the control. BAP had a significant positive impact on betalain production, and increasing BAP and sucrose concentrations generally led to higher betalain production. However, tyrosine was not a significant factor for betalain production in cell suspension cultures. Additionally, antioxidant assays showed that suspension-cultured cells (SCCs) under optimized conditions exhibited free radical scavenging activity comparable to that observed in C. argentea var. plumosa flower extract. This study indicates the potential for further research on betalain production using C. argentea var. plumosa cell cultures, which may have commercial applications.
CRISPR-Based Editing of the Medicago truncatula LEC1 Gene
Authors: Potsenkovskaia Elina A. , Tvorogova Varvara E. , Simonova Veronika Y. , Konstantinov Zakhar S. , Kiseleva Anna S. , Matveenko Andrew G. , Brynchikova Anna V. , Lutova Ludmila A.
Published: November 2024

Arabidopsis thaliana LEAFY COTYLEDON1 (LEC1) gene is shown to have numerous diverse functions in plant development, including the regulation of embryo morphogenesis and maturation, hypocotyl elongation, flowering transition, etc. However, the functions of LEC1 orthologs in different plant species have not been extensively studied. In this study, we obtained a line of Medicago truncatula, a model leguminous plant, carrying the loss-of-function mutation in the MtLEC1 (MtNF-YB10) gene, orthologous to LEC1, using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins (CRISPR/Cas9) genome editing system. Edited plants with loss of MtNF-YB10 function did not demonstrate any severe abnormalities during their normal growth and gave viable seeds, but their capability for somatic embryogenesis in vitro was dramatically reduced. The T1 progeny of unedited plants with a Cas9-gRNA cassette insertion was also analyzed based on the suggestion that editing could occur during seed formation. However, no edited plants were found in the T1 generation. These results suggest divergent functions of LEC1 orthologs and make it possible to investigate potential specific MtNF-YB10 functions.
GC/MS Analysis and Protective Effects of Mentha longifolia L. Essential Oil Against Antituberculosis Drug-Induced Organs Toxicity in Wistar Albino Rats
Authors: Abdel-Hameed Usama K. , Abualghaith Abdulaziz S. , Aly Shaza H. , Soliman Mohamed Mostafa , Munshi Lamiaa Adnan , Mohammed Safia A. A. , Eldahshan Omayma A. , Abdelghffar Eman A. R.
Published: November 2024

Mentha longifolia (L.) L., also known as wild mint, is a perennial herbaceous plant that belongs to the Lamiaceae family. This study aimed to investigate the effects of essential oil of M. longifolia (MLEO) on oxidative stress and inflammatory responses in the liver and kidneys in the context of drug-induced liver injury caused by the anti-TB drugs rifampicin, isoniazid, and pyrazinamide (INH-RIF-PZA). The chemical composition of MLEO was characterized using GC/MS analysis, which revealed the presence of pulegone, trans-p-menthan-3-one, piperitenone, and β-caryophyllene as its major volatile constituents. An INH/RIF/PZA mixture was administered to Wistar rats for 30 days, and silymarin was administered as a standard drug. MLEO was administered p.o. at doses of 50 mg and 100 mg/kg b.w. Both doses of the MLEO therapy effectively regulated all biochemical indicators of hepatic impairment and reduced the damage caused by the INH/RIF/PZA mixture. It may be deduced that MLEO has the ability to protect organs against INH/RIF/PZA-induced damage and could potentially be a valuable natural remedy for treating anti-TB-induced liver and kidney injuries.
Emission and Transcriptional Regulation of Aroma Variation in Oncidium Twinkle ‘Red Fantasy’ Under Diel Rhythm
Authors: Chen Yan , Zhong Shengyuan , Kong Lan , Fan Ronghui , Xu Yan , Chen Yiquan , Zhong Huaiqin
Published: November 2024

Oncidium hybridum is one of the important cut-flowers in the world. However, the lack of aroma in its cut-flower varieties greatly limits the sustainable development of the Oncidium hybridum cut-flowers industry. This paper is an integral investigation of the diel pattern and influencing factors of the aroma release of Oncidium Twinkle ‘Red Fantasy’. GC-MS analysis revealed that the release of 3-Carene peaked at 10:00, while Butyl tiglate and Prenyl senecioate did so at 14:00, with a diel rhythm. By analyzing the correlation network between aroma component synthesis and differentially expressed genes, 15 key structural genes were detected and regulated by multiple circadian rhythm-related transcription factors. Cluster-17371.18_TPS, Cluster-65495.1_TPS, Cluster-46699.0_TPS, Cluster-60935.10_DXS, Cluster-47205.4_IDI, and Cluster-65313.7_LOX were key genes in the terpenoid and fatty acid derivative biosynthetic pathway, which were co-expressed with aroma release. Constant light/dark treatments revealed that the diurnal release of 3-Carene may be influenced by light and the circadian clock, and Butyl tiglate and Prenyl senecioate may be mainly determined by endogenous circadian clock. Under constant light treatment, the TPS, DXS, IDI, and LOX genes seem to lose their regulatory role in the release of aroma compounds from Oncidium Twinkle ‘Red Fantasy’. Under constant dark treatment, the TPS genes were consistent with the release pattern of 3-Carene, which may be a key factor in regulating the diel rhythm of 3-Carene biosynthesis. These results laid a theoretical foundation for the study of floral transcriptional regulation and genetic engineering technology breeding of Oncidium hybridum.
Typical Case Studies and Classification with Evaluation of Carbon Dioxide Geological Sequestration in Saline Aquifers
Authors: Ping Lihua , Wang Huijun , Tian Yuchen , Zhang Helong , Wu Xiuping , Chen Shiheng , Liu Yinghai , Liu Yanzhi , Liu Shiqi , Sang Shuxun , Zheng Sijian
Published: November 2024

To achieve carbon neutrality in China’s fossil energy sector, saline aquifer CO2 geological storage has become a critical strategy. As research into carbon reduction and storage potential evaluation advances across various geological scales, the need arises for consolidating key CO2 storage cases and establishing a standardized classification system and evaluation methodology. This paper provides a comprehensive review of notable CO2 storage projects in saline aquifers, covering aspects such as project overviews, structural and reservoir characteristics, caprock integrity, and seismic monitoring protocols. Drawing on insights from mineral and oil and gas exploration, as well as international methods, this paper outlines the stages and potential levels of saline aquifer storage in China. It proposes an evaluation framework with formulas and reference values for key coefficients. The study includes successful global projects, such as Sleipner and Snøhvit in Norway, In Salah in Algeria, and Shenhua in China’s Ordos Basin, which provide valuable insights for long-term carbon capture and storage (CCS). By examining geological characteristics, injection, and monitoring protocols in these projects, this paper analyzes how geological features impact CO2 storage outcomes. For example, the Sleipner project’s success is linked to its straightforward structure, favorable reservoir properties, and stable caprock, while Snøhvit illustrates diverse structural suitability, and In Salah demonstrates the influence of fractures on storage efficacy. CO2 storage activities are segmented into four stages—survey, investigation, exploration, and injection—and are further categorized by storage potential: geological, technical, techno-economic, and engineering capacities. This study also presents evaluation levels (prediction, control, technically recoverable, and engineering) that support effective reservoir selection, potential classification, and calculations considering factors like reservoir stability and sealing efficacy. Depending on application needs, volumetric or mechanistic methods are recommended, with precise determination of geological, displacement, and cost coefficients. For China, a dynamic evaluation mechanism characterized by multi-scale, tiered approaches and increasing precision over time is essential for robust storage potential assessment. The levels and methods outlined here serve as a scientific foundation for regional and stage-based comparisons, guiding engineering approvals and underground space management. To align with practical engineering demands, ongoing innovation through laboratory experiments, simulations, and field practice is crucial, supporting continual refinement of formulas and key parameter determinations.
The Promising Role of Amine Transaminase Cascades in the Synthesis of Non-Canonical Amino Acids
Authors: Gord Noshahri Najme , Rudat Jens
Published: November 2024

Amine transaminases (ATA) are critical players in producing non-canonical amino acids, essential building blocks in pharmaceuticals and fine chemicals. Significant progress has been made in discovering and engineering enzymes in this field, enhancing their use in organic synthesis. However, challenges such as co-factor regeneration, substrate, and product inhibition remain significant limitations to widespread industrial enzyme application. (Chemo-)enzymatic cascades offer efficient and environmentally friendly pathways for synthesizing amino acids, reducing the need for multiple synthesis steps and saving the purification of intermediates. This review focuses specifically on the synthesis of non-canonical amino acids, emphasizing the use of enzymatic and chemoenzymatic cascades involving ATA.
Numerical Simulation Study on the Stable Combustion of a 660 MW Supercritical Unit Boiler at Ultra-Low Load
Authors: Yang Kaiyu , Li Zhengxin , Cao Xinsheng , Du Tielin , Liu Lang
Published: November 2024

To investigate the safe, stable, and economically viable operation of a boiler under ultra-low-load conditions during the deep peaking process of coal-fired units, a numerical simulation study was conducted on a 660 MW front- and rear-wall hedge cyclone burner boiler. The current research on low load conditions is limited to achieving stable combustion by adjusting the operating parameters, and few effective boiler operating parameter predictions are given for very low-load conditions, i.e., below 20%. Various burner operation modes under ultra-low load conditions were analyzed using computational fluid dynamics (CFDs) methods; this operation was successfully tested with six types of pulverized coal combustion in this paper, and fitting models for outlet flue gas temperature and NOx emissions were derived based on the combustion characteristics of different types of pulverized coal. The results indicate that under 20% ultra-low-load conditions, the use of lower burners leads to a uniform temperature distribution within the furnace, achieving a minimum NOx emission of 112 ppm and a flue gas temperature of 743 K. Coal type 3, with the highest carbon content and a calorific value of 22,440 kJ/kg, has the highest average section temperature of 1435.76 K. In contrast, coal type 1 has a higher nitrogen content, with a maximum cross-sectional average NOx concentration of 865.90 ppm and an exit NOx emission concentration of 800 ppm. The overall lower NOx emissions of coal type 3 are primarily attributed to its reduced nitrogen content and increased oxygen content, which enhance pulverized coal combustion and suppress NOx formation. The fitting models accurately capture the influence of pulverized coal composition on outlet flue gas temperature and NOx emissions. This control strategy can be extended to the stable combustion of many kinds of coal. For validation, the fitting error bar for the predicted outlet flue gas temperature based on the elemental composition of coal type 6 was 8.09%, whereas the fitting error bar for the outlet NOx emissions was only 1.45%.
Isolation and Characterization of Biosurfactant-Producing Bacteria for Enhancing Oil Recovery
Authors: Jiang Meiyu , Wang Hongyi , Liu Jiahui , Hou Xuan , Zhang Yuanyuan , Liu Xiaolin , Wei Shiping , Cui Qingfeng
Published: November 2024

Biosurfactants produced by bacteria possess remarkable emulsification properties for crude oil, significantly enhancing oil mobility and recovery rates. This study aimed to isolate and screen biosurfactant-producing bacteria for oil enhancing recovery. A total of 93 bacterial strains were isolated from marine sediments, with three high-yield biosurfactant-producing strains identified: Pseudomonas aeruginosa N33, Bacillus paralicheniformis Nian2, and Stenotrophomonas nematodicola T10. The fermentation conditions, such as pH, carbon source, nitrogen source, and C/N ratio, were optimized to maximize the yield and activity of biosurfactants. Further evaluations were performed to assess the stability of the bio-surfactant activity and its emulsification properties. The results indicated that all three strains produced biosurfactants that retained their oil displacement activity in the presence of Na+ and Mg2+, but showed a significant reduction in their activities in the presence of Ca2+. The biosurfactants maintained their original activity after treatment at 120 °C for 3 h. Additionally, the biosurfactants produced by all three strains demonstrated excellent oil emulsification capabilities. Static oil-washing and dynamic displacement experiments revealed static oil recovery rates of 28.1%, 23.4%, and 7.1%, respectively, for N33, Nian2, and T10, and dynamic oil displacement recovery rates of 95.0%, 74.1%, and 69.0%, respectively. This research provides valuable microbial resources for enhancing oil recovery via microorganisms and lays a foundation for practical application.
How to Effectively Cool Blade Batteries in Extreme High-Temperature Environments?
Authors: Wang Li , Xia Wenhao , Ding Bin
Published: November 2024

The market share of blade batteries is rising rapidly due to their high energy density, efficient space utilization, and low cost. Nevertheless, effective cooling solutions for blade batteries are crucial to ensure the safe operation of electric vehicles, especially in extreme high-temperature environments. This paper numerically investigates the effects of a cooling plate and the blade battery parameters on maximum battery temperature, maximum temperature difference, and cooling water pressure drop. Additionally, the energy efficiency of these solutions under various cooling demands is analyzed. The numerical results show that increasing the channel number and changing the flow direction does not significantly improve the cooling performance of the cooling plate. Moreover, the effect of cooling water temperature on the maximum temperature difference in blade batteries is negligible. Furthermore, increasing the cooling water mass flow rate and the rotational speed of the cooling fan is preferred when Tmax − Ta > 6 K, while reducing the cooling water temperature is more energy-efficient when Tmax − Ta < 6 K. These results are expected to offer theoretical guidance and data support for designing cooling systems for blade batteries in extreme high-temperature environments.
Biosynthesis; Characterization; and Antibacterial, Antioxidant, and Docking Potentials of Doped Silver Nanoparticles Synthesized from Pine Needle Leaf Extract
Authors: Darwich Nourhane A. , Mezher Malak , Abdallah Alaa M. , El-Sayed Ahmed F. , El Hajj Rana , Hamdalla Taymour A. , Khalil Mahmoud I.
Published: November 2024

The current study focused on the synthesis of doped silver nanoparticles (doped AgNPs) with yttrium (Y), gadolinium (Gd), and chromium (Cr) from pine needle leaf extract (PNLE). X-ray diffraction (XRD) was performed to assess the phase formation, detecting 61.83% from Ag and 38.17% for secondary phases of AgCl, AgO, Y, Cr-, and Gd phases. The size and shape of the NPs were determined by transmission electron microscopy (TEM), showing a spherical shape with an average particle size of 26.43 nm. X-ray photoelectron spectroscopy (XPS) detected the oxidation state of the presented elements. The scanning electron microscope (SEM) and the energy-dispersive X-ray analysis (EDX) determined the morphology and elemental composition of the NPs, respectively. Fourier transform infrared spectroscopy (FTIR) determined the different functional groups indicating the presence of Ag, Y, Gd, Cr, and other groups. Photoluminescence (PL) spectroscopy showed the optical properties of the NPs. A vibrating sample magnetometer (VSM) revealed the ferromagnetic behavior of the doped AgNPs. The antibacterial activity of the doped AgNPs was tested against six uro-pathogenic bacteria (Staphylococcus aureus, Staphylococcus haemolyticus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumonia, and Pseudomonas aeruginosa) using the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) microdilution assays, agar well diffusion assay, time–kill test, and antibiofilm screening assays, revealing significant activity, with MICs ranging between 0.0625 and 0.5 mg/mL and antibiofilm activity between 40 and 85%. The antioxidant activity was determined by the 1,1, diphenyl 1-2 picrylhydrazyl (DPPH) radical scavenging assay with a potential of 61.3%. The docking studies showed that the doped AgNPs had the potential to predict the inhibition of crucial enzymes such as penicillin-binding proteins, LasR-binding proteins, carbapenemase, DNA gyrase, and dihydropteroate synthase. The results suggest that the doped AgNPs can be applied in different medical domains.
Aquinas, Suicide, and Communities of Faith
Authors: McCarty Emily
Published: November 2024

In this paper, I will argue that Aquinas provides a framework for why and how religious communities, specifically Christian religious communities, can minister to those who struggle with suicide. Aquinas thinks that charity makes us friends of God, and to be God’s friend is to love one’s neighbor and care for his needs. After examining what Aquinas has to say about suicide, I consider in some detail what he has to say about charity. In light of recent psychological research, I use what Aquinas has to say about charity to suggest ways in which the church should help those struggling with suicidal ideation.
Effects of Tissue Flossing on Athletic Performance Measures: A Systematic Review
Authors: Cheatham Scott W. , Nadeau Justin , Jackson William , Baker Russell
Published: November 2024

The primary aim of this study was to investigate the efficacy of tissue flossing on athletic performance measures. A secondary aim was to explore the efficacy of tissue flossing when applied to a joint or soft tissue (i.e., muscle belly) on athletic performance measures. An article search was completed in the PubMed/MEDLINE, EBSCO, SCOPUS, and OneSearch electronic databases up to May 2024. Studies were included if they used tissue flossing as a primary intervention among healthy participants and used one or more athletic performance measures as an outcome. Exclusion criteria included studies that did not investigate tissue flossing on athletic performance measures among healthy participants, studies that used tissue flossing for blood flow restriction training, case studies, narrative reviews, dissertations, conference proceedings, and papers written in a language other than English. Eighteen articles and 559 total participants were included in the final analysis. Study quality was assessed by two independent reviewers using the Downs and Black Checklist and the Oxford Centre for Evidence-based Medicine. The major findings suggest that a single tissue flossing treatment ranging from 2 to 10 min that includes active single joint or active closed chain exercises may enhance post-intervention muscle strength, jump performance, and balance up to 45 to 60 min post-intervention. Tissue flossing to a joint or soft tissue both produced mixed results among studies, a definitive answer on which technique is superior cannot be determined at this time. Further direct comparison studies are needed for these two techniques.
Research on Fault Diagnosis of Agricultural IoT Sensors Based on Improved Dung Beetle Optimization–Support Vector Machine
Authors: Liang Sicheng , Liu Pingzeng , Zhang Ziwen , Wu Yong
Published: November 2024

The accuracy of data perception in Internet of Things (IoT) systems is fundamental to achieving scientific decision-making and intelligent control. Given the frequent occurrence of sensor failures in complex environments, a rapid and accurate fault diagnosis and handling mechanism is crucial for ensuring the stable operation of the system. Addressing the challenges of insufficient feature extraction and sparse sample data that lead to low fault diagnosis accuracy, this study explores the construction of a fault diagnosis model tailored for agricultural sensors, with the aim of accurately identifying and analyzing various sensor fault modes, including but not limited to bias, drift, accuracy degradation, and complete failure. This study proposes an improved dung beetle optimization–support vector machine (IDBO-SVM) diagnostic model, leveraging the optimization capabilities of the former to finely tune the parameters of the Support Vector Machine (SVM) to enhance fault recognition under conditions of limited sample data. Case analyses were conducted using temperature and humidity sensors in air and soil, with comprehensive performance comparisons made against mainstream algorithms such as the Backpropagation (BP) neural network, Sparrow Search Algorithm–Support Vector Machine (SSA-SVM), and Elman neural network. The results demonstrate that the proposed model achieved an average diagnostic accuracy of 94.91%, significantly outperforming other comparative models. This finding fully validates the model’s potential in enhancing the stability and reliability of control systems. The research results not only provide new ideas and methods for fault diagnosis in IoT systems but also lay a foundation for achieving more precise, efficient intelligent control and scientific decision-making.
Did ESG Affect the Financial Performance of North American Fast-Moving Consumer Goods Firms in the Second Period of the Kyoto Protocol?
Authors: Helhel Asiyenur , Akgun Eray , Helhel Yesim
Published: November 2024

Many agreements and protocols in the global framework call on industries and businesses to respond to threats related to climate change. New terminologies such as environmental, social, and governance (ESG) scores address this issue and responsibility. This study investigates the impact of sustainability (environment (ENV), social (SOC), governance (GOV), and ESG) on the financial performance of firms in the fast-moving consumer goods industry from 2013 to 2020, the second commitment period of the Kyoto Protocol (SCKP). The study sample covers 113 firms in the North American region (the USA and Canada did not participate in SCKP). The results showed that ESG is not an influencer of financial performance, while ENV and SOC components negatively affect financial performance. On the other hand, GOV is the most significant influencer that positively impacts financial performance. Based on these findings, ESG and its components are not conducive to promoting financial performance during the SCKP period. However, fast-moving consumer goods are ahead of other sectors in terms of sustainability disclosure. Moreover, the highest positive impact of GOV is attributed to the advanced system with rules, standards, and regulations that foster the better and more efficient governance of firms from developed countries.
Systematic Review of Battery Life Cycle Management: A Framework for European Regulation Compliance
Authors: Gianvincenzi Mattia , Marconi Marco , Mosconi Enrico Maria , Favi Claudio , Tola Francesco
Published: November 2024

Batteries are fundamental to the sustainable energy transition, playing a key role in both powering devices and storing renewable energy. They are also essential in the shift towards greener automotive solutions. However, battery life cycles face significant environmental challenges, including the harmful impacts of extraction and refining processes and inefficiencies in recycling. Both researchers and policymakers are striving to improve battery technologies through a combination of bottom–up innovations and top–down regulations. This study aims to bridge the gap between scientific advancements and policy frameworks by conducting a Systematic Literature Review of 177 papers. The review identifies innovative solutions to mitigate challenges across the battery life cycle, from production to disposal. A key outcome of this work is the creation of the life cycle management framework, designed to align scientific developments with regulatory strategies, providing an integrated approach to address life cycle challenges. This framework offers a comprehensive tool to guide stakeholders in fostering a sustainable battery ecosystem, contributing to the objectives set by the European Commission’s battery regulation.
Analysis of Associated Woody and Semi-Woody Local Wild Species in Entre Ríos, Argentina: Exploring the Agricultural Potential of Hexachlamys edulis
Authors: Povilonis Ignacio Sebastián , Arena Miriam Elisabet , Alonso Marta , Radice Silvia
Published: November 2024

The loss of native forests in Argentina has been a concern, driven by factors such as agriculture expansion and urbanization. Therefore, understanding the conservation status of sampled populations and their adaptation to different plant communities is essential. This research focused on the heterogeneity analysis of the associated woody and semi-woody vegetation to Hexachlamys edulis (O. Berg) Kausel and D. Legrand, a species commonly known as “ubajay” in Entre Ríos, Argentina. The study aimed to record the species present in the populations, explore plant communities associated with H. edulis, identify other potentially useful agroforestry species, compare locations based on the similarity of accompanying species, and explain the conservation status of each population. Results revealed a total of 71 species belonging to 39 families. The Myrtaceae family was the most relevant, particularly in terms of native species representation. The analysis of biodiversity indicators, including richness, the Shannon index, and dominance revealed variations among the studied sites. The anthropic indicator highlighted the impact of human activity, with Concordia showing a higher ratio of native-to-exotic species. Cluster analysis and ordination techniques revealed groupings of censuses from the same localities, indicating differences in vegetation composition between sites. Significant differences in species composition were found among the sampled populations. Overall, the study can serve as baseline information for future research on the dynamics of vegetation in these areas and on the studied H. edulis species. Finally, these findings contribute to understanding how wild species like H. edulis adapt to different plant communities, which might be valuable for developing new agroecological approaches or identifying potential companion planting species in future agricultural systems.
Advances, Hotspots, and Trends in Outdoor Education Research: A Bibliometric Analysis
Authors: Zong Bobo , Sun Yifan , Li Linfeng
Published: November 2024

Utilizing the Web of Science database as a retrieval source, this study employs CiteSpace software to conduct a visualization analysis of 8380 documents related to outdoor education published from 1994 to 2023. The findings reveal a phased increase in the volume of outdoor education research, with a shift in research themes from environmental governance to environmental education, ultimately concentrating on education for sustainable development that is characterized by significant temporal features. Initially dominated by publications from Europe and North America, the geographical distribution of research has gradually expanded globally. The core research theme centres around environmental education, with experiential education, outdoor learning, and education for sustainable development evolving concurrently. The network structure of research collaboration predominantly involves higher education institutions, with a noticeable shift from limited disciplinary research to interdisciplinary integration across multiple fields.
Integrating Lean Management and Circular Economy for Sustainable Dentistry
Authors: Antoniadou Maria
Published: November 2024

The increasing focus on environmental sustainability has prompted the healthcare sector, including dentistry, to reconsider waste management practices. Dental offices generate various waste streams from outdated technologies, including single-use plastics, hazardous materials, and digital waste. This scoping review explores how integrating circular economy principles with lean management practices can enhance sustainability and operational efficiency in dental clinics. Using the PRISMA methodology, the review identifies 30 articles that passed the Cochrane Risk of Bias Tool and the Critical Appraisal Skills Program (CASP) checklist from 2000. Circular economy principles focus on reducing waste and maximizing resource reuse, while lean management seeks to improve efficiency and minimize process waste. Together, these approaches can significantly reduce resource consumption and enhance recycling efforts in dental practices. Despite barriers such as high technology costs, regulatory constraints, and limited recycling infrastructure, case studies highlight successful implementation in healthcare settings, demonstrating improved environmental and operational outcomes. Collaboration between dental professionals, policymakers, and industry leaders is essential for promoting the adoption of these sustainable practices.
Transforming Irrigated Agriculture in Semi-Arid and Dry Subhumid Mediterranean Conditions: A Case of Protected Cucumber Cultivation
Authors: Darwish Talal , Shaban Amin , Faour Ghaleb , Jomaa Ihab , Moubarak Peter , Khadra Roula
Published: November 2024

Pressure from population growth and climate change stress the limited water resources in the Mediterranean region and threaten food security and social stability. Enhancing food production requires the transformation of irrigation systems and enhancement of local capacity for sustainable water and soil management in irrigated agriculture. The aim of this work is the conversion of traditional irrigation practices, by introducing the practice of optimal irrigation scheduling based on local ET estimation and soil moisture monitoring, and the use of continuous feeding by fertigation to enhance both water and nutrient use efficiency. For this, two trials were established between August and November 2023 in two different pedoclimatic zones (Serein and Sultan Yacoub) of the inner Bekaa Plain of Lebanon, characterized by semi-arid and dry subhumid conditions and different soil types. Greenhouse cucumber was tested to compare the prevailing traditional farmers’ practices with the advanced, technology-based, methods of water management. Results showed a significantly higher amount of water applied by the farmers to the protected cucumber, with a potential for average saving of 105 mm of water applied in each season by improved practices. Water input in the traditional practices revealed potential stress to plants. With more than 20% increase in cucumber yield by the transformed practices, a general trend was observed in the fertilization approach and amounts, resulting in lower nutrient recovery in the farmer’s plots. The science-based practices of water and nutrient management showed higher application and agronomic water use efficiency of full fertigation, exceeding 60%, associated with double and triple higher nitrogen use efficiency, compared to those results obtained by the traditional water and fertilizer application methods. The monitored factors can contribute to severe economic and environmental consequences from nutrient buildup or leaching in the soil–groundwater system in the Mediterranean region.
Seasonal Dynamics and Source Apportionment of Heavy Metals in Road Dust: A Case Study of Liuzhou, China
Authors: Zhang Lu , Qian Jianping , Liu Jinrui , Niu Kangkang , Zhang Huanrong
Published: November 2024

The spatial distribution and seasonal variation in heavy metal pollution in road dust in Liuzhou, China, were investigated. Road dust samples were collected during both drought and wet periods, and the concentrations of nine heavy metals—Cr, Ni, Cu, Pb, Zn, Cd, As, Sb, and Hg—were analyzed. The analysis showed that all nine heavy metals were higher than the background values of Chinese urban soils in both the drought and wet periods, and the mean heavy metal contents of road dust in the dry period were higher than those in the wet period, except for Cd. In the assessment of the pollution of heavy metals in road dust, the results of Igeo showed that Cd and Hg were significant; the results of NI showed that Cr and Ni were more significant. The spatial analysis shows that the industrial concentration areas in the north and west of Liuzhou City are concentration areas of heavy metal pollution. A Pearson correlation analysis showed high correlation between Cd in road dust heavy metals and Cd in the surrounding soil. Source identification via PCA revealed four main contributors: metallurgical and coal-fired industries, mechanical manufacturing, green belt maintenance, and waste treatment facilities. A quantitative analysis via APCS-MLR modeling confirmed that metallurgical and coal-fired industrial sources are the most widespread and important pollution sources in Liuzhou. There is a significant increase in the contribution of waste treatment sources to Hg contamination during wet periods.
On the Problem of the Uniqueness of Fixed Points and Solutions for Quadratic Fractional-Integral Equations on Banach Algebras
Authors: Cichoń Kinga , Cichoń Mieczysław , Ciesielski Maciej
Published: November 2024

In this paper, we study the problem of the uniqueness of fixed points for operators defined on subspaces of the space of continuous functions C[a,b] equipped with norms stronger than the supremum norm. We are particularly interested in Hölder spaces since they are natural ranges of integral operators of fractional order. Our goal is to preserve the expected regularity of the fixed points (solutions of the equations) when investigating their uniqueness, without assuming a contraction condition on the space under study. We claim some symmetry between the case of the obtained results and the case of the classical Banach fixed-point theorem in such spaces, even for operators which are not necessarily contractions in the sense of the norm of these subspaces. This result is of particular interest for the study of quadratic integral equations, and as an application example we prove the uniqueness theorem for such a kind equations with general fractional order integral operators, which are not necessarily contractions, in a suitably constructed generalized Hölder space.