bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of Miniaturized Wearable Wristband Type Surface EMG Measurement System for Biometric Authentication
Authors: Shin Siho , Kang Mingu , Jung Jaehyo , Kim Youn Tae
Published: April 2021

Personal authentication systems employing biometrics are attracting increasing attention owing to their relatively high security compared to existing authentication systems. In this study, a wearable electromyogram (EMG) system that can be worn on the forearm was developed to detect EMG signals and, subsequently, apply them for personal authentication. In previous studies, wet electrodes were attached to the skin for measuring biosignals. Wet electrodes contain adhesives and conductive gels, leading to problems such as skin rash and signal-quality deterioration in long-term measurements. The miniaturized wearable EMG system developed in this study comprised flexible dry electrodes attached to the watch strap, enabling EMG measurements without additional electrodes. In addition, for accurately classifying and applying the measured signal to the personal authentication system, an optimal algorithm for classifying the EMG signals based on a multi-class support vector machine (SVM) model was implemented. The model using cubic SVM achieved the highest personal authentication rate of 87.1%. We confirmed the possibility of implementing a wearable authentication system by measuring the EMG signal and artificial intelligence analysis algorithm presented in this study.
Comparison of Different Design Alternatives for Hardware-in-the-Loop of Power Converters
Authors: Zamiri Elyas , Sanchez Alberto , Yushkova Marina , Martínez-García Maria Sofia , de Castro Angel
Published: April 2021

This paper aims to compare different design alternatives of hardware-in-the-loop (HIL) for emulating power converters in Field Programmable Gate Arrays (FPGAs). It proposes various numerical formats (fixed and floating-point) and different approaches (pure VHSIC Hardware Description Language (VHDL), Intellectual Properties (IPs), automated MATLAB HDL code, and High-Level Synthesis (HLS)) to design power converters. Although the proposed models are simple power electronics HIL systems, the idea can be extended to any HIL system. This study compares the design effort of different coding methods and numerical formats considering possible synthesis tools (Precision and Vivado), and it comprises an analytical discussion in terms of area and speed. The different models are synthesized as ad-hoc modules in general-purpose FPGAs, but also using the NI myRIO device as an example of a commercial tool capable of implementing HIL models. The comparison confirms that the optimum design alternative must be chosen based on the application (complexity, frequency, etc.) and designers’ constraints, such as available area, coding expertise, and design effort.
Unsupervised Subcategory Domain Adaptive Network for 3D Object Detection in LiDAR
Authors: Wang Zhiyu , Wang Li , Xiao Liang , Dai Bin
Published: April 2021

Three-dimensional object detection based on the LiDAR point cloud plays an important role in autonomous driving. The point cloud distribution of the object varies greatly at different distances, observation angles, and occlusion levels. Besides, different types of LiDARs have different settings of projection angles, thus producing an entirely different point cloud distribution. Pre-trained models on the dataset with annotations may degrade on other datasets. In this paper, we propose a method for object detection using an unsupervised adaptive network, which does not require additional annotation data of the target domain. Our object detection adaptive network consists of a general object detection network, a global feature adaptation network, and a special subcategory instance adaptation network. We divide the source domain data into different subcategories and use a multi-label discriminator to assign labels dynamically to the target domain data. We evaluated our approach on the KITTI object benchmark and proved that the proposed unsupervised adaptive method could achieve a remarkable improvement in the adaptation capabilities.
Disposable over Reusable Face Masks: Public Safety or Environmental Disaster?
Authors: Prata Joana C. , Silva Ana L. Patrício , Duarte Armando C. , Rocha-Santos Teresa
Published: April 2021

Many governments have imposed the public use of face masks and they are now moving towards enforcing disposable masks to abate COVID-19 transmission. While disposable masks consistently provide higher protection, they also carry multiple environmental burdens, from greenhouse gases released during production to the landfilling and littering. Conversely, reusable masks’ protection can vary from >90% certified industrial masks, similar to disposable masks, to dubious homemade or artisanal masks. This work discusses the protection provided by different masks, their impact on the environment, and new proposals combining concerns about public health and sustainability.
Modeling and Spatialization of Biomass and Carbon Stock Using LiDAR Metrics in Tropical Dry Forest, Brazil
Authors: Oliveira Cinthia Pereira de , Ferreira Rinaldo Luiz Caraciolo , da Silva José Antônio Aleixo , Lima Robson Borges de , Silva Emanuel Araújo , Silva Anderson Francisco da , Lucena Josias Divino Silva de , dos Santos Nattan Adler Tavares , Lopes Iran Jorge Corrêa , Pessoa Mayara Maria de Lima , Melo Cybelle Laís Souto-Maior Sales de
Published: April 2021

In recent years, with the growing environmental concern regarding climate change, there has been a search for efficient alternatives in indirect methods for the quantification of biomass and forest carbon stock. In this article, we seek to obtain pioneering results of biomass and carbon estimates from forest inventory data and LiDAR technology in a dry tropical forest in Brazil. We use forest inventory data in two areas together with data from the LiDAR flyby, generating estimates of local biomass and carbon levels obtained from local species. We approach three types of models for data analysis: Multiple linear regression with principal components (PCA), conventional multiple linear regression and stepwise multiple linear regression. The best fit total above ground biomass (TAGB) and total above ground carbon (TAGC) model was the stepwise multiple linear regression, concluding, then, that LiDAR data can be used to estimate biomass and total carbon in dry tropical forest, proven by an adjustment considered in the models employed, with a significant correlation between the LiDAR metrics. Our finding provides important information about the spatial distribution of TAGB and TAGC in the study area, which can be used to manage the reserve for optimal carbon sequestration.
Saccharomyces cerevisiae Fermentation of 28 Barley and 12 Oat Cultivars
Authors: Tse Timothy J. , Wiens Daniel J. , Shen Jianheng , Beattie Aaron D. , Reaney Martin J. T.
Published: April 2021

As barley and oat production have recently increased in Canada, it has become prudent to investigate these cereal crops as potential feedstocks for alcoholic fermentation. Ethanol and other coproduct yields can vary substantially among fermented feedstocks, which currently consist primarily of wheat and corn. In this study, the liquified mash of milled grains from 28 barley (hulled and hull-less) and 12 oat cultivars were fermented with Saccharomyces cerevisiae to determine concentrations of fermentation products (ethanol, isopropanol, acetic acid, lactic acid, succinic acid, α-glycerylphosphorylcholine (α-GPC), and glycerol). On average, the fermentation of barley produced significantly higher amounts of ethanol, isopropanol, acetic acid, succinic acid, α-GPC, and glycerol than that of oats. The best performing barley cultivars were able to produce up to 78.48 g/L (CDC Clear) ethanol and 1.81 g/L α-GPC (CDC Cowboy). Furthermore, the presence of milled hulls did not impact ethanol yield amongst barley cultivars. Due to its superior ethanol yield compared to oats, barley is a suitable feedstock for ethanol production. In addition, the accumulation of α-GPC could add considerable value to the fermentation of these cereal crops.
Gasification of Woody Biomasses and Forestry Residues: Simulation, Performance Analysis, and Environmental Impact
Authors: Safarian Sahar , Ebrahimi Saryazdi Seyed Mohammad , Unnthorsson Runar , Richter Christiaan
Published: April 2021

Wood and forestry residues are usually processed as wastes, but they can be recovered to produce electrical and thermal energy through processes of thermochemical conversion of gasification. This study proposes an equilibrium simulation model developed by ASPEN Plus to investigate the performance of 28 woody biomass and forestry residues’ (WB&FR) gasification in a downdraft gasifier linked with a power generation unit. The case study assesses power generation in Iceland from one ton of each feedstock. The results for the WB&FR alternatives show that the net power generated from one ton of input feedstock to the system is in intervals of 0 to 400 kW/ton, that more that 50% of the systems are located in the range of 100 to 200 kW/ton, and that, among them, the gasification system derived by tamarack bark significantly outranks all other systems by producing 363 kW/ton. Moreover, the environmental impact of these systems is assessed based on the impact categories of global warming (GWP), acidification (AP), and eutrophication (EP) potentials and normalizes the environmental impact. The results show that electricity generation from WB&FR gasification is environmentally friendly for 75% of the studied systems (confirmed by a normalized environmental impact [NEI] less than 10) and that the systems fed by tamarack bark and birch bark, with an NEI lower than 5, significantly outrank all other systems owing to the favorable results obtained in the environmental sector.
Implementation of a Virtual Assistant for the Academic Management of a University with the Use of Artificial Intelligence
Authors: Villegas-Ch William , García-Ortiz Joselin , Mullo-Ca Karen , Sánchez-Viteri Santiago , Roman-Cañizares Milton
Published: April 2021

Currently, private universities, as a result of the pandemic that the world is facing, are going through very delicate moments in several areas, both academic and financial. Academically, there are learning problems and these are directly related to the dropout rate, which brings financial problems. Added to this are the economic problems caused by the pandemic, where the rates of students who want to access a private education have dropped considerably. For this reason, it is necessary for all private universities to have support to improve their student income and avoid cuts in budgets and resources. However, the academic part represents a great effort to fulfill their academic activities, which are the priority, with attention on those interested in pursuing a training programs. To solve these problems, it is important to integrate technologies such as Chatbots, which use artificial intelligence in such a way that tasks such as providing information on an academic courses are addressed by them, reducing the administrative burden and improving the user experience. At the same time, this encourages people to be a part of the college.
Validation of Antiobesity Effects of Black Soybean Seed Coat Powder Suitable as a Food Material: Comparisons with Conventional Yellow Soybean Seed Coat Powder
Authors: Moriyasu Yuuki , Fukumoto Chiho , Wada Maki , Yano Erika , Murase Hiroshi , Mizuno Masatoshi , Zaima Nobuhiro , Moriyama Tatsuya
Published: April 2021

In this study, we fed obese model mice black soybean seed coat powder (BSCP) and evaluated the antiobesity effects. As a control, normal yellow soybean seed coat powder (YSCP) was used. C57BL/6J, a high-fat diet-induced obesity model mouse, was fed a high-fat diet containing BSCP or YSCP (20% fat) to induce obesity. The results showed that in the BSCP group, it caused significant suppression of body weight gain and suppression of white adipose tissue weight compared with the YSCP group. Moreover, it significantly decreased serum leptin levels, which correlated with visceral fat mass, and increased antidiabetic adipocytokine and adiponectin levels. Therefore, this suggests the pigmented components contained in BSCP have an antiobesity effect in obese model mice. It is suggested that this material, which can be prepared without extraction with an organic solvent and is suitable for use as a food material, could be a functional food material with a practicable antiobesity effect.
Which Company Characteristics Make a Food Business at Risk for Food Fraud?
Authors: van Ruth Saskia M. , Nillesen Onno
Published: April 2021

Fraud can happen to any food business, but some sectors show more historical evidence of food fraud than others. This may be due to particular company characteristics that affect a company’s level of vulnerability. In the current study, we examined the relevance of the industry segment, business size, and location of food businesses on their food fraud vulnerabilities. Over 8000 food fraud vulnerability self-assessments conducted by food businesses active in 20 industry segments located in five continents were collected and the data analyzed. Results revealed that a company’s industry segment (chain and tier) affects its fraud vulnerability greatly and to a larger extent than the size of the business. The effect of industry segment on fraud vulnerability appears fairly similar across continents, whereas the effect of business size exhibits large geographical variation. The results demonstrate that those involved in animal product supply chains and end of chain nodes (catering, retail) are most vulnerable, and so are larger businesses, and businesses located in Africa and Asia. Current results imply that company characteristics are important determinants of the level of fraud vulnerability, and they may be used reversely in the future, i.e., as predictors of vulnerability.
An Approach to Investigate Content-Related Quality of Nutraceuticals Used by Slovenian Consumers: A Case Study with Folate and Vitamin D Supplements
Authors: Žmitek Katja , Krušič Sanja , Pravst Igor
Published: April 2021

A predisposition for the efficiency of nutraceuticals is that the product contains a sufficient quantity of a vitamin. Several studies have highlighted different quality issues. Our objective was to investigate whether the contents of the vitamins in selected types of food supplements were in accordance with labeling. We focused on two types of food supplements where content-related quality issues could result in public health risks: food supplements for supplementation with (a) folic acid (as 5-methyltetrahydrofolate (5-MTHF)) in pregnancy and (b) with vitamin D in the general population. The study was done on supplements from the global supply that are typically used by Slovenian consumers. We sampled one production batch of 30 different food supplements—six and 24 samples with 5-MTHF and cholecalciferol, respectively. We found samples with vitamin contents outside the 80–150% tolerance interval in both sets. Particularly, 5-MTHF was found to be more problematic, probably due to its lower stability. This study shows the need for better quality control. Quality control is needed during both the manufacturing process and product shelf lifetimes. Content quality should be also subject to external controls by authorities. Voluntarily quality control schemes would also enable consumers to identify products of sufficient quality.
BTH Treatment Delays the Senescence of Postharvest Pitaya Fruit in Relation to Enhancing Antioxidant System and Phenylpropanoid Pathway
Authors: Ding Xiaochun , Zhu Xiaoyang , Zheng Wang , Li Fengjun , Xiao Shuangling , Duan Xuewu
Published: April 2021

The plant resistance elicitor Benzo (1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) can enhance disease resistance of harvested fruit. Nonetheless, it is still unknown whether BTH plays a role in regulating fruit senescence. In this study, exogenous BTH treatment efficiently delayed the senescence of postharvest pitaya fruit with lower lipid peroxidation level. Furthermore, BTH-treated fruit exhibited lower hydrogen peroxide (H2O2) content, higher contents of reduced ascorbic acid (AsA) and reduced glutathione (GSH) levels and higher ratios of reduced to oxidized glutathione (GSH/GSSG) and ascorbic acid (AsA/DHA), as well as higher activities of ROS scavenging enzymes, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD) and glutathione reductase (GR) in comparison with control fruit. Moreover, BTH treatment enhanced the activities of phenylpropanoid pathway-related enzymes, including cinnamate-4-hydroxylase (C4H), phenylalanine ammonia-lyase (PAL) and 4-coumarate/coenzyme A ligase (4CL) and the levels of phenolics, flavonoids and lignin. In addition, BTH treatment upregulated the expression of HuSOD1/3/4, HuCAT2, HuAPX1/2 and HuPOD1/2/4 genes. These results suggested that application of BTH delayed the senescence of harvested pitaya fruit in relation to enhanced antioxidant system and phenylpropanoid pathway.
Quality of Frozen Hake Fillets in the Portuguese Retail Market: A Case Study of Inadequate Practices in the European Frozen White Fish Market
Authors: Mendes Rogério , Silva Helena , Oliveira Patrícia , Oliveira Luís , Teixeira Bárbara
Published: April 2021

The overall quality of frozen hake fillets in the Portuguese market was evaluated. Physical, biochemical, microbiological, and sensory analysis in 20 brands revealed several non-conformities. Hake was identified in 19 brands, although two mislabeled the species. Lower net weight than labeled was evidenced in ca. one-third of brands. TVB-N in ca. one-third of the samples presented high values, although within legal limit. Almost all brands presented excessive amount of ice glaze, low levels of WHC (raw/cooked), low levels of soluble protein, and undue thaw-drip loss, thus reflecting the poor quality of fillets. Added phosphates were below the legal limit in all samples; however, they were used in glaze ice in three brands, and only labeled in one. Overall microbiological quality of frozen fillets was good, though yeasts and molds detected in six samples indicate poor hygienic conditions in some facilities. Labels comply with legal requisites, however, need improvement, namely the ‘best before’ periods. In general, packaging was efficient regarding presence of ice crystals and dehydration signs. Concerning sensory analysis of cooked fillets, 63% of the brands had bad to poor quality and 37% fair quality. Merluccius productus revealed the worst quality, namely regarding thaw drip loss, thaw drip loss protein, M/P ratio, pH and sensory evaluation. Overall results show that a significant part of the hake fillets business operators are still failing in relation with best practices.
Stability and Median Rationalizability for Aggregate Matchings
Authors: Echenique Federico , Lee SangMok , Shum Matthew , Yenmez M. Bumin
Published: April 2021

We develop the theory of stability for aggregate matchings used in empirical studies and establish fundamental properties of stable matchings including the result that the set of stable matchings is a non-empty, complete, and distributive lattice. Aggregate matchings are relevant as matching data in revealed preference theory. We present a result on rationalizing a matching data as the median stable matching.
Genome-Wide Transcriptional Analysis and Functional Validation Linked a Cluster of Epsilon Glutathione S-Transferases with Insecticide Resistance in the Major Malaria Vector Anopheles funestus across Africa
Authors: Kouamo Mersimine F. M. , Ibrahim Sulaiman S. , Hearn Jack , Riveron Jacob M. , Kusimo Michael , Tchouakui Magellan , Ebai Terence , Tchapga Williams , Wondji Murielle J. , Irving Helen , Boudjeko Thaddée , Boyom Fabrice F. , Wondji Charles S.
Published: April 2021

Resistance is threatening the effectiveness of insecticide-based interventions in use for malaria control. Pinpointing genes associated with resistance is crucial for evidence-based resistance management targeting the major malaria vectors. Here, a combination of RNA-seq based genome-wide transcriptional analysis and RNA-silencing in vivo functional validation were used to identify key insecticide resistance genes associated with DDT and DDT/permethrin cross-resistance across Africa. A cluster of glutathione-S-transferase from epsilon group were found to be overexpressed in resistant populations of Anopheles funestus across Africa including GSTe1 [Cameroon (fold change, FC: 2.54), Ghana (4.20), Malawi (2.51)], GSTe2 [Cameroon (4.47), Ghana (7.52), Malawi (2.13)], GSTe3 [Cameroon (2.49), Uganda (2.60)], GSTe4 in Ghana (3.47), GSTe5 [Ghana (2.94), Malawi (2.26)], GSTe6 [Cameroun (3.0), Ghana (3.11), Malawi (3.07), Uganda (3.78)] and GSTe7 (2.39) in Ghana. Validation of GSTe genes expression profiles by qPCR confirmed that the genes are differentially expressed across Africa with a greater overexpression in DDT-resistant mosquitoes. RNAi-based knock-down analyses supported that five GSTe genes are playing a major role in resistance to pyrethroids (permethrin and deltamethrin) and DDT in An. funestus, with a significant recovery of susceptibility observed when GSTe2, 3, 4, 5 and GSTe6 were silenced. These findings established that GSTe3, 4, 5 and 6 contribute to DDT resistance and should be further characterized to identify their specific genetic variants, to help design DNA-based diagnostic assays, as previously done for the 119F-GSTe2 mutation. This study highlights the role of GSTes in the development of resistance to insecticides in malaria vectors and calls for actions to mitigate this resistance.
“Is Cleopatra Black?”: Examining Whiteness and the American New Woman
Authors: Maier Angelica J.
Published: April 2021

In the 1920s and 1930s, conceptions of the “New Woman” and Egyptomania shaped American culture. Employing methods of critical race art history and material culture studies, I focus on a 1925 Callot Soeurs dress and silk pajamas (c. 1920–1929), taking into consideration both the semiotic qualities of Egyptian motifs as they circulated in early twentieth century American visual culture as well as the sensuous material aspects of the garments. Through primary sources like cosmetic advertisements, fashion magazines, and costume manuals, I contextualize the figure of Cleopatra as a symbol of white beauty and power in this period. Weighing both visual and material aspects, I argue that the repeated act of wearing these garments by white-presenting women placed them in a performative valence, where the wearer ironically became a white woman through her appropriation of Cleopatra and Egyptian motifs. Further, these motifs conferred modernity, cosmopolitanism, class status and an acceptable sexuality upon the wearer. As such, I address how material objects shape subjectivity, simultaneously reflecting and producing racialized and gendered discourses. By focusing on white womanhood, I draw upon critical studies of whiteness in order to disrupt its invisible normative status. This essay traces its operational logic and aids in dismantling the pervasive power of white supremacy that continues to circulate today.
Is Greater Than 0.5 MAC Inhalational Agent Use Post-Bypass Related to Need for Inotropic and/or Vasoconstrictor Support?
Authors: Lenk Tara A. , Guerra-Londono Carlos E. , Graul Thomas E. , Murinson Marc A. , Hehar Prabhdeep K. , Divine George , Modak Raj K.
Published: April 2021

Background and Aims: We hypothesized that maintaining a patient on moderate–high doses of potent inhalational agent for greater than 30 min during the post-bypass period would be an independent predictor of initiation and usage of either inotropic and/or vasopressor infusions. Setting and Design: This study is a retrospective design and approved by the institutional review board. The setting was a single-center, academic tertiary care hospital in Detroit, Michigan. Materials and Methods: Three-hundred, ninety-seven elective cardiac surgery patients were identified for chart review. Electronic medical records were reviewed to collect demographics and perioperative data. Statistics used include a propensity score regression adjusted analysis utilizing logistic regression models and a multivariable model. Results: A propensity score regression adjusted analysis was performed and then applied in both univariate and multivariate logistic regression models with a p value of <0.05 reaching statistical significance. Fifty-six percent of the participants had an exposure of greater than 30 min of a minimum alveolar concentration of isoflurane greater than 0.5 (ETISO ≥ 0.5MAC, 30 min) in the post-bypass period. After adjusting for propensity score, this was found to be a significant predictor of inotrope and/or vasoconstrictor use post-bypass (OR 2.49, 95% CI 1.15–5.38, p = 0.021). In the multivariate model, pulmonary hypertension (OR 5.9; 95% CI 1.33–26.28; p = 0.02), Euroscore II (2.73; 95% CI 1.35–5.5; p = 0.005), and cardiopulmonary bypass hours (OR 1.86; 95% CI 1.02–3.4; p = 0.042) emerged as significant. Conclusions: This study showed that an ETISO ≥ 0.5MAC, 30 min exposure during the immediate post-bypass period during elective cardiac surgery was an independent predictor of a patient being started on inotrope or vasoconstrictor infusions. Further research should consider a prospective design and examine depth of anesthesia during the post-bypass period.
Integrative Statistics, Machine Learning and Artificial Intelligence Neural Network Analysis Correlated CSF1R with the Prognosis of Diffuse Large B-Cell Lymphoma
Authors: Carreras Joaquim , Kikuti Yara Yukie , Miyaoka Masashi , Roncador Giovanna , Garcia Juan Fernando , Hiraiwa Shinichiro , Tomita Sakura , Ikoma Haruka , Kondo Yusuke , Ito Atsushi , Komohara Yoshihiro , Nakamura Naoya , Hamoudi Rifat
Published: April 2021

Tumor-associated macrophages (TAMs) of the immune microenvironment play an important role in the Diffuse Large B-cell Lymphoma (DLBCL) pathogenesis. This research aimed to characterize the expression of macrophage colony-stimulating factor 1 receptor (CSF1R) at the gene and protein level in correlation with survival. First, the immunohistochemical expression of CSF1R was analyzed in a series of 198 cases from Tokai University Hospital and two patterns of histological expression were found, a TAMs, and a diffuse B-lymphocytes pattern. The clinicopathological correlations showed that the CSF1R + TAMs pattern associated with a poor progression-free survival of the patients, disease progression, higher MYC proto-oncogene expression, lower MDM2 expression, BCL2 translocation, and a MYD88 L265P mutation. Conversely, a diffuse CSF1R + B-cells pattern was associated with a favorable progression-free survival. Second, the histological expression of CSF1R was also correlated with 10 CSF1R-related markers including CSF1, STAT3, NFKB1, Ki67, MYC, PD-L1, TNFAIP8, IKAROS, CD163, and CD68. CSF1R moderately correlated with STAT3, TNFAIP8, CD68, and CD163 in the cases with the CSF1R + TAMs pattern. In addition, machine learning modeling predicted the CSF1R immunohistochemical expression with high accuracy using regression, generalized linear, an artificial intelligence neural network (multilayer perceptron), and support vector machine (SVM) analyses. Finally, a multilayer perceptron analysis predicted the genes associated with the CSF1R gene expression using the GEO GSE10846 DLBCL series of the Lymphoma/Leukemia Molecular Profiling Project (LLMPP), with correlation to the whole set of 20,683 genes as well as with an immuno-oncology cancer panel of 1790 genes. In addition, CSF1R positively correlated with SIRPA and inversely with CD47. In conclusion, the CSF1R histological pattern correlated with the progression-free survival of the patients of the Tokai series, and predictive analytics is a feasible strategy in DLBCL.
Oxygen Supply System Management in an Overweight Adult after 12 Months in Antarctica—Study Case
Authors: Radziejowska Maria , Moiseyenko Yevgen , Radziejowski Paweł , Zych Michał
Published: April 2021

The aim of the study was to try to determine the functional state of the respiratory system, i.e., selected parameters and indicators of physiological systems responsible for the supply of oxygen at all stages of its delivery in people as their body weight increases from normal weight to overweight. The studies include an analysis of test results of functional respiratory system state (FSD) indicators of a 30-year-old and 170-cm tall man. Measurements of FSD were conducted two times: the first time before an expedition to Antarctica at 70 kg (normal body weight); the next measurements were taken a year later, after coming back from the expedition, at 82 kg (overweight). When analyzing the functional respiratory system state in terms of the effect of overweight it was found that the maintenance of the oxygen homeostasis in those conditions occurred at the level of a compensated hypoxic state. That is why the decision to engage in physical activity can be made only if we are sure that significant destructive additive effects of both types of hypoxic influences (from excessive body weight and from the physical activity) are not overlapping.
Correction: Cervinka, R.; et al. Investigating the Qualities of a Recreational Forest: Findings from the Cross-Sectional Hallerwald Case Study. Int. J. Environ. Res. Public Health 2020, 17, 1676
Authors: Cervinka Renate , Schwab Markus , Haluza Daniela
Published: April 2021

In the original article [...]
Vibration Exposure and Transmissibility on Dentist’s Anatomy: A Study of Micro Motors and Air-Turbines
Authors: Banga Harish Kumar , Goel Pankaj , Kumar Raman , Kumar Vikas , Kalra Parveen , Singh Sehijpal , Singh Sunpreet , Prakash Chander , Pruncu Catalin
Published: April 2021

The use of dental hand pieces endanger dentists to vibration exposure as they are subjected to very high amplitude and vibration frequency. This paper has envisaged a comparative analysis of vibration amplitudes and transmissibility during idling and drilling with micro motor (MM) and air-turbine (AT) hand pieces. The study aims to identify the mean difference in vibration amplitudes during idling, explore different grasp forces while drilling with irrigant injection by the dentist, and various vibration transmission of these hand pieces. The study utilized 22 separate frequency resonances on two new and eight used MMs and two new and eight used ATs of different brands by observing the investigator at 16 different dentist clinics. The study adopted a descriptive research design with non–probability sampling techniques for selecting dentists and hand pieces. Statistical methods like Levene Test of Homogeneity, Welch ANOVA, independent t-test, and Games–Howell test were utilized with SPSS version 22 and MS-Excel. The results reveal that vibration amplitudes and vibration transmissibility when measured at position 2 are higher than in another position 1. Vibrations during idling for used MMs are more than AT hand pieces, and the used MM (MUD) and used AT (AUA) hand pieces differ due to their obsolescence and over-usage. Vibration amplitudes increase every time with the tightening of grasping of the hand piece. Vibration amplitudes for each grasping style of MM hand piece differ from all other grasping styles of AT hand pieces. Routine exposure to consistent vibrations has ill physical, mental, and psychological effects on dentists. The used hand pieces more hazardous as compared to newer ones. The study suggests that these hand pieces must be replaced periodically, sufficient to break between two operations, especially after every hand piece usage. Hence, the present research work can be further extended by creating some control groups among dentists and then studying the vibration amplitude exposure of various dental hand pieces and subsequent transmissibility to their body parts.
The Influence of Abortion Law on the Frequency of Pregnancy Terminations—A Retrospective Comparative Study
Authors: Zaręba Kornelia , Wójtowicz Stanisław , Banasiewicz Jolanta , Herman Krzysztof , Jakiel Grzegorz
Published: April 2021

Abortion law is one of the main factors influencing the number of abortions performed in a country. The study aimed to assess the influence of abortion law on the number of performed terminations with particular attention paid to pregnancy terminations due to fetal defects. The retrospective comparative analysis of statistical data included on the governmental websites of Poland and the UK was performed. The average of 190,733.1 terminations were performed in the United Kingdom in the years 2009–2018 with the average of 2820.9 due to fetal defects. At the same time the average of 858.6 terminations were performed in Poland with 820.7 due to fetal defects. Population size is the only significant predictor of the number of terminations in the United Kingdom. The increase in the number of deliveries and population in Poland was not linked to the increase in the overall number of terminations or terminations due to fetal defects. It might be due to the unavailability of pregnancy terminations in many places. The radicalization of abortion law exerts no influence on the decrease in the number of terminations due to fetal indications. The liberalization of abortion law promotes the increase in the number of terminations due to social indications.
Suicide Thoughts and Attempts in the Norwegian General Population during the Early Stage of the COVID-19 Outbreak
Authors: Bonsaksen Tore , Skogstad Laila , Heir Trond , Ekeberg Øivind , Schou-Bredal Inger , Grimholt Tine K.
Published: April 2021

The aim of the study was to examine the prevalence of suicide thoughts and attempts during the early stage of the COVID-19 outbreak and examine pandemic-related factors associated with suicide thoughts in the general Norwegian population. A sample of 4527 adults living in Norway were recruited via social media. Data related to suicide thoughts and attempts, alcohol use, pandemic-related concerns, and sociodemographic variables were collected. Associations with suicide thoughts were analyzed with logistic regression analysis. In the sample, 3.6% reported suicide thoughts during the last month, while 0.2% had attempted suicide during the same period. Previous suicide attempts (OR: 11.93, p < 0.001), lower age (OR: 0.69, p < 0.001), daily alcohol use (OR: 3.31, p < 0.001), being in the risk group for COVID-19 complications (OR: 2.15, p < 0.001), and having economic concerns related to the pandemic (OR: 2.28, p < 0.001) were associated with having current suicide thoughts. In addition to known risk factors, the study suggests that aspects specific to COVID-19 may be important for suicidal behaviors during the pandemic.
Does International Travel Frequency Affect COVID-19 Biosecurity Behavior in the United States?
Authors: Kim Myung Ja , Hall C. Michael , Bonn Mark
Published: April 2021

High-quality biosecurity practices are critical to restarting international tourism. Effective market segmentation improves the communication and efficacy of health advice. Travel frequency is an important basis for health-related consumer segmentation, as it is closely related to risk of greater exposure to infectious diseases. Theoretically grounded studies of tourist biosecurity behavior and travel frequency have largely been neglected, although insights into practices and attitudes are especially relevant for coronavirus disease of 2019 (COVID-19 (coronavirus disease of 2019) health responses. Therefore, this research constructed and tested a conceptual model applying Value–Attitude–Behavior theory to US travelers to see whether the frequency of international travel affected tourist COVID-19 related biosecurity behavior. US respondents were drawn from a panel using a quota sampling technique according to the age and gender of American outbound tourists. An online survey was administered in September 2020. The responses (n = 395) of those who traveled internationally within five years were analyzed utilizing partial least squares-structural equation modeling (PLS-SEM) with multi-group analysis. Travel frequency significantly affects biosecurity behavior. High travel frequency (≥8 trips) has the strongest effect of value on biosecurity attitudes, personal norms, social norms, and biosecurity social norms, leading to biosecurity behaviors. Biosecurity behaviors pertaining to medium travel frequency (4–7 trips) are significantly influenced by personal norms. At low travel frequency (1–3 trips) levels, biosecurity behaviors are stimulated by biosecurity attitudes and social norms, showing the highest predictive power among the three groups. This work provides insights into international travel consumer biosecurity practices and behavior. From a market segmentation perspective, the levels of international travel frequency have various influences on biosecurity values, attitudes, personal norms, social norms, and behaviors. The biosecurity behaviors of low-frequency travelers are found to be the most significant of the three groups, suggesting that individuals who travel less frequently are more likely to practice responsible COVID-19 biosecurity behavior.
Modelling Sediment Retention Services and Soil Erosion Changes in Portugal: A Spatio-Temporal Approach
Authors: Marques Susana M. , Campos Felipe S. , David João , Cabral Pedro
Published: April 2021

Soils provide important regulating ecosystem services and have crucial implications for human well-being and environmental conservation. However, soil degradation and particularly soil erosion jeopardize the maintenance and existence of these services. This study explores the spatio–temporal relationships of soil erosion to understand the distribution patterns of sediment retention services in mainland Portugal. Based on Corine Land Cover maps from 1990 to 2018, the InVEST Sediment Delivery Ratio (SDR) model was used to evaluate the influence of sediment dynamics for soil and water conservation. Spatial differences in the sediment retention levels were observed within the NUTS III boundaries, showing which areas are more vulnerable to soil erosion processes. Results indicated that the Region of Leiria, Douro and the coastal regions have decreased importantly in sediment retention capacity over the years. However, in most of the territory (77.52%), changes in sediment retention were little or were not important (i.e., less than 5%). The statistical validation of the model proved the consistency of the results, demonstrating that the InVEST SDR model is an appropriate tool for estimating soil loss potential by water at regional/national levels, although having its limitations. These findings can be relevant to support strategies for more efficient land-use planning regarding soil erosion mitigation practices and to stimulate further investigation at a national level on this important ecosystem service.
DNA2 in Chromosome Stability and Cell Survival—Is It All about Replication Forks?
Authors: Hudson Jessica J. R. , Rass Ulrich
Published: April 2021

The conserved nuclease-helicase DNA2 has been linked to mitochondrial myopathy, Seckel syndrome, and cancer. Across species, the protein is indispensable for cell proliferation. On the molecular level, DNA2 has been implicated in DNA double-strand break (DSB) repair, checkpoint activation, Okazaki fragment processing (OFP), and telomere homeostasis. More recently, a critical contribution of DNA2 to the replication stress response and recovery of stalled DNA replication forks (RFs) has emerged. Here, we review the available functional and phenotypic data and propose that the major cellular defects associated with DNA2 dysfunction, and the links that exist with human disease, can be rationalized through the fundamental importance of DNA2-dependent RF recovery to genome duplication. Being a crucial player at stalled RFs, DNA2 is a promising target for anti-cancer therapy aimed at eliminating cancer cells by replication-stress overload.
Marliolide Derivative Induces Melanosome Degradation via Nrf2/p62-Mediated Autophagy
Authors: Yun Cheong-Yong , Choi Nahyun , Lee Jae Un , Lee Eun Jung , Kim Ji Young , Choi Won Jun , Oh Sang Ho , Sung Jong-Hyuk
Published: April 2021

Nuclear factor erythroid 2-related factor 2 (Nrf2), which is linked to autophagy regulation and melanogenesis regulation, is activated by marliolide. In this study, we investigated the effect of a marliolide derivative on melanosome degradation through the autophagy pathway. The effect of the marliolide derivative on melanosome degradation was investigated in α-melanocyte stimulating hormone (α-MSH)-treated melanocytes, melanosome-incorporated keratinocyte, and ultraviolet (UV)B-exposed HRM-2 mice (melanin-possessing hairless mice). The marliolide derivative, 5-methyl-3-tetradecylidene-dihydro-furan-2-one (DMF02), decreased melanin pigmentation by melanosome degradation in α-MSH-treated melanocytes and melanosome-incorporated keratinocytes, evidenced by premelanosome protein (PMEL) expression, but did not affect melanogenesis-associated proteins. The UVB-induced hyperpigmentation in HRM-2 mice was also reduced by a topical application of DMF02. DMF02 activated Nrf2 and induced autophagy in vivo, evidenced by decreased PMEL in microtubule-associated proteins 1A/1B light chain 3B (LC3)-II-expressed areas. DMF02 also induced melanosome degradation via autophagy in vitro, and DMF02-induced melanosome degradation was recovered by chloroquine (CQ), which is a lysosomal inhibitor. In addition, Nrf2 silencing by siRNA attenuated the DMF02-induced melanosome degradation via the suppression of p62. DMF02 induced melanosome degradation in melanocytes and keratinocytes by regulating autophagy via Nrf2-p62 activation. Therefore, Nrf2 activator could be a promising therapeutic agent for reducing hyperpigmentation.
In Vitro Efficacy of Bacterial Cellulose Dressings Chemisorbed with Antiseptics against Biofilm Formed by Pathogens Isolated from Chronic Wounds
Authors: Dydak Karolina , Junka Adam , Dydak Agata , Brożyna Malwina , Paleczny Justyna , Fijalkowski Karol , Kubielas Grzegorz , Aniołek Olga , Bartoszewicz Marzenna
Published: April 2021

Local administration of antiseptics is required to prevent and fight against biofilm-based infections of chronic wounds. One of the methods used for delivering antiseptics to infected wounds is the application of dressings chemisorbed with antimicrobials. Dressings made of bacterial cellulose (BC) display several features, making them suitable for such a purpose. This work aimed to compare the activity of commonly used antiseptic molecules: octenidine, polyhexanide, povidone-iodine, chlorhexidine, ethacridine lactate, and hypochlorous solutions and to evaluate their usefulness as active substances of BC dressings against 48 bacterial strains (8 species) and 6 yeast strains (1 species). A silver dressing was applied as a control material of proven antimicrobial activity. The methodology applied included the assessment of minimal inhibitory concentrations (MIC) and minimal biofilm eradication concentration (MBEC), the modified disc-diffusion method, and the modified antibiofilm dressing activity measurement (A.D.A.M.) method. While in 96-well plate-based methods (MIC and MBEC assessment), the highest antimicrobial activity was recorded for chlorhexidine, in the modified disc-diffusion method and in the modified A.D.A.M test, povidone-iodine performed the best. In an in vitro setting simulating chronic wound conditions, BC dressings chemisorbed with polyhexanide, octenidine, or povidone-iodine displayed a similar or even higher antibiofilm activity than the control dressing containing silver molecules. If translated into clinical conditions, the obtained results suggest high applicability of BC dressings chemisorbed with antiseptics to eradicate biofilm from chronic wounds.
Alterations of the Skin and Gut Microbiome in Psoriasis and Psoriatic Arthritis
Authors: Olejniczak-Staruch Irmina , Ciążyńska Magdalena , Sobolewska-Sztychny Dorota , Narbutt Joanna , Skibińska Małgorzata , Lesiak Aleksandra
Published: April 2021

Numerous scientific studies in recent years have shown significant skin and gut dysbiosis among patients with psoriasis. A significant decrease in microbiome alpha-diversity (abundance of different bacterial taxa measured in one sample) as well as beta-diversity (microbial diversity in different samples) was noted in psoriasis skin. It has been proven that the representation of Cutibacterium, Burkholderia spp., and Lactobacilli is decreased and Corynebacterium kroppenstedii, Corynebacterium simulans, Neisseria spp., and Finegoldia spp. increased in the psoriasis skin in comparison to healthy skin. Alterations in the gut microbiome in psoriasis are similar to those observed in patients with inflammatory bowel disease. In those two diseases, the F. prausnitzii, Bifidobacterium spp., Lactobacillus spp., Parabacteroides and Coprobacillus were underrepresented, while the abundance of Salmonella sp., Campylobacter sp., Helicobacter sp., Escherichia coli, Alcaligenes sp., and Mycobacterium sp. was increased. Several research studies provided evidence for the significant influence of psoriasis treatments on the skin and gut microbiome and a positive influence of orally administered probiotics on the course of this dermatosis. Further research is needed to determine the influence of the microbiome on the development of inflammatory skin diseases. The changes in microbiome under psoriasis treatment can serve as a potential biomarker of positive response to the administered therapy.
Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry and High-Sensitivity Gas Chromatography-Mass Spectrometry Screening of Classic Drugs and New Psychoactive Substances and Metabolites in Urine of Consumers
Authors: Marchei Emilia , Ferri Maria Alias , Torrens Marta , Farré Magí , Pacifici Roberta , Pichini Simona , Pellegrini Manuela
Published: April 2021

The use of the new psychoactive substances is continuously growing and the implementation of accurate and sensible analysis in biological matrices of users is relevant and fundamental for clinical and forensic purposes. Two different analytical technologies, high-sensitivity gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) were used for a screening analysis of classic drugs and new psychoactive substances and their metabolites in urine of formed heroin addicts under methadone maintenance therapy. Sample preparation involved a liquid-liquid extraction. The UHPLC-HRMS method included Accucore™ phenyl Hexyl (100 × 2.1 mm, 2.6 μm, Thermo, USA) column with a gradient mobile phase consisting of mobile phase A (ammonium formate 2 mM in water, 0.1% formic acid) and mobile phase B (ammonium formate 2 mM in methanol/acetonitrile 50:50 (v/v), 0.1% formic acid) and a full-scan data-dependent MS2 (ddMS2) mode for substances identification (mass range 100–1000 m/z). The GC-MS method employed an ultra-Inert Intuvo GC column (HP-5MS UI, 30 m, 250 µm i.d, film thickness 0.25 µm; Agilent Technologies, Santa Clara, CA, USA) and electron-impact (EI) mass spectra were recorded in total ion monitoring mode (scan range 40–550 m/z). Urine samples from 296 patients with a history of opioid use disorder were examined. Around 80 different psychoactive substances and/or metabolites were identified, being methadone and metabolites the most prevalent ones. The possibility to screen for a huge number of psychotropic substances can be useful in suspected drug related fatalities or acute intoxication/exposure occurring in emergency departments and drug addiction services.