bluuebunny/crossref_metadata_2025 huggingface dataset:
Ambassador of People with Disabilities in the Workplace—Conducive Demographic and Professional Characteristics
Authors: Załuska Urszula , Grześkowiak Alicja , Kozyra Cyprian , Kwiatkowska-Ciotucha Dorota
Published: September 2020

This paper introduces into the analysis the concept of the ambassador of people with disability in the workplace. A kind and friendly person in the workplace, who creates a positive atmosphere around people with disabilities, may play a crucial role in their adaptation on the open labor market. Presence of such a person is especially important in entities that did not previously employ people with disabilities. It is vital that employers who would like to employ people with disability possess knowledge about demographic and professional characteristics that predispose employees to perform this special role. On the one hand, in this article we attempted to evaluate the differentiation in the perception of the issue of disability due to demographic and professional characteristics of respondents, and, on the other hand, to identify features that favor being an “ambassador of people with disabilities” in the workplace. The study was conducted in 2019 on the representative samples of Internet users from 8 European countries using Computer-Assisted Internet Interviews. For the purposes of the study, we used the Attitudes to Disability Scale WHOQOL Group test and a proprietary questionnaire. As for the methods of analysis, we relied on the classical analysis of variance and logistic regression. The conducted study showed that the perception of the issue of disability is significantly related to demographic and professional characteristics of respondents, and that the role of the ambassador is the most appropriate for a middle-aged woman with a good knowledge of disability issues, indecisive in the workplace.
Managers’ Conceptions and Their Effects on the Perception of Employees with Disabilities
Authors: Marques Antônio Luiz , Romeo Marina , Matalinares Marjorye , Yepes-Baldó Montserrat
Published: September 2020

The research aimed to identify managers’ conceptions of disability and the relationship that was established between these conceptions and their perception of the persons with disabilities (PWD) performance, bond, benefits of hiring, and training needs. 257 managers answered a questionnaire in order to identify conceptions of disability in organizations. Descriptive statistics, factorial analysis, and hierarchical analysis of grouping were performed while using IBM Statistic 20.0.0. The results show that managers who have the spiritual and the conception based on inclusion perceive the insertion of PWD as beneficial to the organization. Those who conceive disability as a question of normality perceive the PWD performance as inferior to those without disabilities, which implies that PWDs should be segregated; and, the managers who perceive disability as a social problem are likely to place PWDs in the workplace according to their potential. The results can be fruitfully used by managers, human resources’ professionals, academics, and the society to promote inclusion.
Profiles of Problematic Internet Use in Bullying and Cyberbullying among Adolescents
Authors: Méndez Inmaculada , Jorquera Ana Belén , Esteban Cecilia Ruiz , García-Fernández José Manuel
Published: September 2020

The rise of technology has increased risks such as problematic internet use or cyberbullying. Data show that there is problematic use of the internet, which has important repercussions academically, personally, socially and for health. The objective of this study was to identify different profiles that vary according to intra- and interpersonal conflicts related to internet use. In addition, this study aimed to examine whether there are significant differences in bullying and cyberbullying among adolescents with a conflict related to internet use. The study participants were 810 students of Compulsory Secondary Education (M = 13.99, SD = 1.32). The Questionnaire on School Violence and the Questionnaire of Experiences Related to Internet was use. The latent profile analysis identified four different types of conflicts related to internet use: (a) high levels in intra- and interpersonal conflicts; (b) low levels intra- and interpersonal conflicts; (c) moderate intra- and interpersonal conflicts and (d) very high levels in intra- and interpersonal conflicts. The results of the study indicated that there were significant differences in the manifestations of school violence between the profiles. This study assists in educational programs to prevent conflicts related to internet use and school violence through emotional adjustment.
Care Dependency in Patients with Heart Failure: A Cross-Sectional Study in Spain
Authors: Juárez-Vela Raúl , Durante Ángela , Pellicer-García Begoña , Cardoso-Muñoz Antonio , Criado-Gutiérrez José María , Antón-Solanas Isabel , Gea-Caballero Vicente
Published: September 2020

Background: Heart failure (HF) is a progressive and debilitating condition that represents an ever-growing problem for health systems worldwide. HF patients feel that they are a burden on their families, they feel socially isolated and have a low perception of their health. Accordingly, the objectives of this study were to: (1) to explore the profile of care dependency in a representative sample of Spanish HF patients through the Care Dependency Scale (CDS), and (2) to identify correlates of care dependency in this population. Material and Methods: We performed a cross-sectional study of 187 patients admitted for HF decompensation to the Hospital Clínico of Zaragoza (Spain). Results: Only 15% of our sample was highly or completely dependent on care from others. More specifically, our results indicate that HF patients felt a greater level of dependency on care from others when it comes to moving, getting dressed and undressed, maintaining good personal hygiene, participating in daily and recreational activities and being continent. Conclusions: We find association between the CDS categories that present a low score for care dependency in HF patients and the patients’ physical deterioration.
Impacts of a Standing Desk Intervention within an English Primary School Classroom: A Pilot Controlled Trial
Authors: Sherry Aron P. , Pearson Natalie , Ridgers Nicola D. , Johnson William , Barber Sally E. , Bingham Daniel D. , Nagy Liana C. , Clemes Stacy A.
Published: September 2020

Traditional classroom furniture dictates that children predominantly sit during class time. This study evaluated the impact of providing standing desks within a deprived UK primary school setting over 8 months using mixed-method approaches. All children within a Year 5 class (9–10-year-olds, n = 30) received an adjustable sit–stand desk, while another Year 5 class (n = 30) in a nearby school retained traditional furniture as a control classroom. At baseline, 4 months, and 8 months, activPAL monitors (PAL Technologies, Glasgow, UK) were worn for 7 days to provide time spent sitting and standing. Behavior-related mental health, musculoskeletal discomfort surveys, and a cognitive function test battery were also completed at all three timepoints. Intervention experiences from pupils and the teacher were captured using focus groups, interviews, and classroom observations. At both 4 months and 8 months, multi-level models revealed a reduction in class time sitting in the intervention group compared to the control group ((β (95%CI) 4 months −25.3% (−32.3, −18.4); 8 months −19.9% (−27.05, −12.9)). Qualitative data revealed challenges to teaching practicalities and a gradual decline in behavior-related mental health was observed (intervention vs. control: 4 months +5.31 (+2.55, +8.08); 8 months +7.92 (+5.18, +10.66)). Larger trials within similar high-priority settings are required to determine the feasibility and cost-effectiveness of providing standing desks to every child in the classroom.
Predictive Modeling for Occupational Safety Outcomes and Days Away from Work Analysis in Mining Operations
Authors: Yedla Anurag , Kakhki Fatemeh Davoudi , Jannesari Ali
Published: September 2020

Mining is known to be one of the most hazardous occupations in the world. Many serious accidents have occurred worldwide over the years in mining. Although there have been efforts to create a safer work environment for miners, the number of accidents occurring at the mining sites is still significant. Machine learning techniques and predictive analytics are becoming one of the leading resources to create safer work environments in the manufacturing and construction industries. These techniques are leveraged to generate actionable insights to improve decision-making. A large amount of mining safety-related data are available, and machine learning algorithms can be used to analyze the data. The use of machine learning techniques can significantly benefit the mining industry. Decision tree, random forest, and artificial neural networks were implemented to analyze the outcomes of mining accidents. These machine learning models were also used to predict days away from work. An accidents dataset provided by the Mine Safety and Health Administration was used to train the models. The models were trained separately on tabular data and narratives. The use of a synthetic data augmentation technique using word embedding was also investigated to tackle the data imbalance problem. Performance of all the models was compared with the performance of the traditional logistic regression model. The results show that models trained on narratives performed better than the models trained on structured/tabular data in predicting the outcome of the accident. The higher predictive power of the models trained on narratives led to the conclusion that the narratives have additional information relevant to the outcome of injury compared to the tabular entries. The models trained on tabular data had a lower mean squared error compared to the models trained on narratives while predicting the days away from work. The results highlight the importance of predictors, like shift start time, accident time, and mining experience in predicting the days away from work. It was found that the F1 score of all the underrepresented classes except one improved after the use of the data augmentation technique. This approach gave greater insight into the factors influencing the outcome of the accident and days away from work.
Evaluation of the Nutritional Quality of Processed Foods in Honduras: Comparison of Three Nutrient Profiles
Authors: Hernandez Santana Adriana , Waleska Bodden Andrade Sharyl , Rojas Aleman Dina , Enríquez Jean Pierre , Di Iorio Adriana Beatriz
Published: September 2020

Obesity is considered a global pandemic. Different countries have worked to implement front-of-package (FOP) labeling systems that define thresholds for critical nutrients (CN) as part of their public health policies. The objective of this study is to identify the proportion of Processed (PF) and Ultra-Processed (UPF) Foods marketed in Honduras, which meet or fall short of the criteria of three Nutrient Profile Models (NPM): PAHO (2016), Chile (2017) and the Central American Technical Regulation Proposal-Nutritional Warning Front Labeling (CATRP-NWFL 2017). This study is descriptive; 1009 products from 206 brands were collected nationwide. Descriptive statistics were performed. The mean CN compliance with the three models was 49.3% for sodium, 30.6% for sugars, 63.1% and 96% for saturated and trans fats. The PAHO and Chilean (NPM) similarly concentrated on the lower compliance with the established criteria, unlike the CATRP, which has less stringent criteria. This is the first assessment of CN content in PF and UPF in Honduras under three different NPMs. We highlight the importance of defining or adopting criteria for the implementation of NWFL as information for the consumer and thereby contribute to reducing the risks of obesity and related diseases.
Downside: The Perpetrator of Violence in the Representations of Social and Health Professionals
Authors: Procentese Fortuna , Fasanelli Roberto , Carnevale Stefania , Esposito Ciro , Pisapia Noemi , Arcidiacono Caterina , Napoli Immacolata Di
Published: September 2020

Gender-based violence is a widespread phenomenon and pandemic that affects women’s lives. Many interventions have been activated for perpetrators, but the dropout rate is still high. In order to draw up guidelines for responsibly and sustainably dealing with the phenomenon, this study is aimed at investigating the professionals’ perception of the perpetrator as a useful element in designing innovative intervention policies. Open interviews were carried out with welfare and health professionals and the Grounded Theory Methodology was used to analyze the collected data. These results detect attitudes of social health personnel and their feelings of impotence towards gender-based perpetrators because of the emergence of an inevitable repetitiveness of the violent behavior, as well as the “normality of violence” in a patriarchal culture and its “transversality”. This reflective knowledge allows for the opportunity to develop best transformative attitudes toward the phenomenon. According to the results, it is urgent to establish an active and convinced alliance with the healthy part of the man, through specific prevention paths, in order to activate an authentic motivation for change and its sustainability.
Thinking about My Existence during COVID-19, I Feel Anxiety and Awe—The Mediating Role of Existential Anxiety and Life Satisfaction on the Relationship between PTSD Symptoms and Post-Traumatic Growth
Authors: Tomaszek Katarzyna , Muchacka-Cymerman Agnieszka
Published: September 2020

Background: The global outbreak of COVID-19set new challenges and threats for every human being. In the psychological field it is similar to deep existential crises or a traumatic experience that may lead to the appearance or exacerbation of a serious mental disorder and loss of life meaning and satisfaction. Courtney et al. (2020) discussed deadly pandemic COVID-19 in the light of TMT theory and named it as global contagion of mortality that personally affected every human being. Such unique conditions activate existential fears as people start to be aware of their own mortality. Objective: The main aim of this study was to test the mediating effect of existential anxiety, activated by COVID-19 and life satisfaction (SWLS) on the relationship between PTSD symptoms and post-traumatic growth (PTG). We also examined the moderated mediating effect of severity of trauma symptoms on life satisfaction and existential anxiety and its associations with PTG. Method: We conducted an online survey during the peak of the COVID-19 outbreak in Poland. The participants completed existential anxiety scale (SNE), life satisfaction scale (SWLS), IES-R scale for measuring the level of PTSD symptoms and post-traumatic growth inventory (PTGI). Results: The effect of PTSD on PTG was found to be mediated by existential anxiety and life satisfaction. We also confirmed two indirect effects: (1) the indirect effect of PTSD on PTG via existential anxiety and life satisfaction tested simultaneously; (2) the indirect effect of life satisfaction on PTG through severity of trauma symptoms. An intermediate or high level of PTSD level was related to less PTG when low and full PTSD stress symptoms strengthened PTG experiences. Conclusions: A therapeutic intervention for individuals after traumatic experience should attempt to include fundamental existential questions and meaning of life as well as the severity of PTSD symptoms. The severity of traumatic sensations may affect the relationship between life satisfaction and post-traumatic growth.
Even Pacing Is Associated with Faster Finishing Times in Ultramarathon Distance Trail Running—The “Ultra-Trail du Mont Blanc” 2008–2019
Authors: Suter Daniel , Sousa Caio Victor , Hill Lee , Scheer Volker , Nikolaidis Pantelis Theo , Knechtle Beat
Published: September 2020

In recent years, there has been an increasing number of investigations analyzing the effects of sex, performance level, and age on pacing in various running disciplines. However, little is known about the impact of those factors on pacing strategies in ultramarathon trail running. This study investigated the effects of age, sex, and performance level on pacing in the UTMB® (Ultra-trail du Mont Blanc) and aimed to verify previous findings obtained in the research on other running disciplines and other ultramarathon races. Data from the UTMB® from 2008 to 2019 for 13,829 race results (12,681 men and 1148 women) were analyzed. A general linear model (two-way analysis of variance (ANOVA)) was applied to identify a sex, age group, and interaction effect in pace average and pace variation. A univariate model (one-way ANOVA) was used to identify a sex effect for age, pace average, and pace variation for the fastest men and women. In our study, pace average and a steadier pace were positively correlated. Even pacing throughout the UTMB® correlated with faster finishing times. The average pace depended significantly on sex and age group. When considering the top five athletes in each age group, sex and age group also had significant effects on pace variation. The fastest women were older than the fastest men, and the fastest men were faster than the fastest women. Women had a higher pace variation than men. In male competitors, younger age may be advantageous for a successful finish of the UTMB®. Faster male runners seemed to be younger in ultramarathon trail running with large changes in altitude when compared to other distances and terrains.
Household CO2 Emissions: Current Status and Future Perspectives
Authors: Liu Lina , Qu Jiansheng , Maraseni Tek Narayan , Niu Yibo , Zeng Jingjing , Zhang Lihua , Xu Li
Published: September 2020

The household sector, which plays a critical role in emission reduction, is a main source of greenhouse gas (GHG) emissions. Although numerous academic journals have published papers on household CO2 emissions (HCEs), great challenges remain in research on assessments, determinants, and further research prospects. This work reviews and projects HCEs using a bibliometric analysis and a systematic review based on the data from the Web of Science (WOS) platform from 1991 to 2020. Over the last 30 years, there has been a rapid and active trend of research on HCEs. We find that (1) the scale of the bibliometric analysis shows that research on HCEs is interdisciplinary and must consider overall cognition of the environment, the economy, society, and technology. It also needs to strengthen cooperation between different countries/territories to emphasize the quality and influence of papers on HCEs. (2) A review of previous literature shows that research on HCEs mainly focuses on the research object, mainstream assessments, and influencing factors. The following six main aspects impact HCEs: demographic, income, social, technological, policy, and natural factors. (3) The research discussion suggests that more micro-level research needs to be conducted, such as research on the city level and the individual level, which is important for sustainable development and low consumption. A comparative analysis of the differences in HCEs is a future research direction. Additionally, localized carbon emission reduction measures need to be implemented.
Non-Malignant Respiratory Illnesses in Association with Occupational Exposure to Asbestos and Other Insulating Materials: Findings from the Alberta Insulator Cohort
Authors: Moitra Subhabrata , Farshchi Tabrizi Ali , Idrissi Machichi Kawtar , Kamravaei Samineh , Miandashti Noushin , Henderson Linda , Mukherjee Manali , Khadour Fadi , Naseem Muhammad T. , Lacy Paige , Melenka Lyle
Published: September 2020

Many insulating materials are used in construction, although few have been reported to cause non-malignant respiratory illnesses. We aimed to investigate associations between exposures to insulating materials and non-malignant respiratory illnesses in insulators. In this cross-sectional study, 990 insulators (45 ± 14 years) were screened from 2011–2017 in Alberta. All participants underwent pulmonary function tests and chest radiography. Demographics, work history, and history of chest infections were obtained through questionnaires. Chronic obstructive pulmonary disease (COPD) was diagnosed according to established guidelines. Associations between exposures and respiratory illnesses were assessed by modified Poisson regression. Of those screened, 875 (88%) were males. 457 (46%) participants reported having ≥ 1 chest infection in the past 3 years, while 156 (16%) were diagnosed with COPD. In multivariate models, all materials (asbestos, calcium silicate, carbon fibers, fiberglass, and refractory ceramic fibers) except aerogels and mineral fibers were associated with recurrent chest infections (prevalence ratio [PR] range: 1.18–1.42). Only asbestos was associated with COPD (PR: 1.44; 95% confidence interval [CI]: 1.01, 2.05). Therefore, occupational exposure to insulating materials was associated with non-malignant respiratory illnesses, specifically, recurrent chest infections and COPD. Longitudinal studies are urgently needed to assess the risk of exposure to these newly implemented insulation materials.
Orthodontics First in Hemimandibular Hyperplasia. “Mind the Gap”
Authors: Perrotta Stefania , Lo Giudice Giorgio , Bocchino Tecla , Califano Luigi , Valletta Rosa
Published: September 2020

A 32-year-old man was referred to the Division of Orthodontics of the University of Naples “Federico II”, with a 15-year history of gradually increasing right-sided facial asymmetry. Clinical and radiological examinations was consistent to hemimandibular hyperplasia, a rare developmental asymmetry characterized by three-dimensional enlargement of one-half of the mandible. The standard surgical-orthodontic management was proposed to the patient. However, he refused to undergo bimaxillary orthognatic surgery. Therefore, a different treatment was proposed based on the orthodontic technique of pre-surgical decompensation and post-surgical refinement used in bimaxillary orthognatic surgery planning, and surgical intervention with a condylectomy. The dental arches were evenly levelled out with a multi-bracket treatment and then the condylectomy was performed. Orthodontic treatment continued with a levelling and torque control by 0.19 × 0.25 SS arch and physiotherapy. At the three-month follow-up, the patient showed anterior and posterior bite rebalancing, arch intercuspation recovery, and anterior open bite closure due to muscular self-rebalancing. The two-year follow-up showed regular mandibular dynamic, orthodontic appliances were removed, and the patient was instructed to wear retainer for the following months. The final result was aesthetically reasonable for the patient, although slight asymmetry of the chin persisted.
Tuberculosis and Other Airborne Microbes in Occupational Health and Safety
Authors: Vaquero-Álvarez Esther , Cubero-Atienza Antonio , Ruiz-Martínez Pilar , Vaquero-Abellán Manuel , Redel-Macías María Dolores , Aparicio-Martínez Pilar
Published: September 2020

Airborne pathogens and non-malignant infectious diseases such as tuberculosis are highly contagious and can have severe effects on healthcare workers. The symptoms of these diseases take time to manifest, which can prevent workers from noticing that they have been exposed until symptoms appear. The current paper sought to assess the occupational safety and preventative measures taken in laboratories in Spain, and to compare these measures with those reported by other studies worldwide. A cross-sectional study of workers (35–50 years old) was conducted using a web survey (N = 30), and a bibliometric analysis was carried out in the Scopus database (92 documents were selected). The occupational safety and health measures were inadequate, according to the opinions of the workers. The training (p < 0.01), the amount of work (p < 0.05), and how the workers followed their protocols (p < 0.001) were linked to incidents and exposure to airborne pathogens. The most significant previous publication was a report (848 citations) stating that the previous variables linked to exposure are vital for prevention. Most works focused on countries like the U.S.A. (p = 0.009) were reviews, with a limited number of studies focused on occupational safety.
Model Construction for Undergraduate Student College Adjustment
Authors: Lee Sona , Ahn Hye Young
Published: September 2020

Background: College students are known to struggle with a number of difficulties, such as their future careers and interpersonal relationships, as well as job-seeking stress. This study aimed to develop and test a structural model for undergraduate student college adjustment. Methods: The data collection period ranged from November 2019 to January 2020. A questionnaire was distributed to a total of 300 college students; a total of 290 copies were ultimately used for analysis. Result: The model fit indexes of the final model were χ2 = 427.707 (p < 0.001), DF = 173, χ2/DF = 2.47, GFI = 0.88, Adjusted Goodness of Fit Index (AGFI) = 0.84, Comparative Fit Index (CFI) = 0.91, Incremental Fit Index (IFI) = 0.92, Standardized Root Mean-square Residual (SRMR) = 0.07, and Root Mean-Square Error of Approximation (RMSEA) = 0.07. All of the model fit indexes were acceptable, and seven of the thirteen paths in the final model were significant. The factors that influenced college adjustment were social support (r = 0.39) and ego-identity (β = 0.73), explaining 57.1% of the variance. Conclusions: To increase college adjustment, it is necessary to consider the relationship of adjustment with college students’ surrounding environments, such as their family, friends, and professors, and how students can improve their ego-identity.
Effect of Early Mobilization on Physical Function in Patients after Cardiac Surgery: A Systematic Review and Meta-Analysis
Authors: Kanejima Yuji , Shimogai Takayuki , Kitamura Masahiro , Ishihara Kodai , Izawa Kazuhiro P.
Published: September 2020

The objective effects of early mobilization on physical function in patients after cardiac surgery remain unknown. The purpose of the present study was to clarify the effects of early mobilization on physical function in patients after cardiac surgery through meta-analysis. Four electronic databases were searched on 2 August 2019. We used search keywords related to “early mobilization”, “cardiac surgery”, and “randomized controlled trials”. All randomized controlled trials conducting early mobilization after cardiac surgery were included. We defined early mobilization as the application of physical activity within the first five postoperative days. Citations and data extraction were independently screened in duplicate by two authors. The meta-analysis was conducted using random-effects modeling with EZR software. The primary outcome was the distance walked during the six-minute walking test at hospital discharge. Six randomized controlled trials comprising 391 patients were included following screening of 591 studies. All studies included coronary artery bypass grafting as the cardiac surgery conducted. Early mobilization started on postoperative days 1–2 and was conducting twice daily. Early mobilization showed a trend of being combined with respiratory exercise or psychoeducation. The meta-analysis showed that the distance walked during the 6-min walking test improved by 54 m (95% confidence interval, 31.1–76.9; I2 = 52%) at hospital discharge. The present study suggested that early mobilization after cardiac surgery may improve physical function at discharge.
Glacial Lakes Mapping Using Multi Satellite PlanetScope Imagery and Deep Learning
Authors: Qayyum Nida , Ghuffar Sajid , Ahmad Hafiz , Yousaf Adeel , Shahid Imran
Published: September 2020

Glacial lakes mapping using satellite remote sensing data are important for studying the effects of climate change as well as for the mitigation and risk assessment of a Glacial Lake Outburst Flood (GLOF). The 3U cubesat constellation of Planet Labs offers the capability of imaging the whole Earth landmass everyday at 3–4 m spatial resolution. The higher spatial, as well as temporal resolution of PlanetScope imagery in comparison with Landsat-8 and Sentinel-2, makes it a valuable data source for monitoring the glacial lakes. Therefore, this paper explores the potential of the PlanetScope imagery for glacial lakes mapping with a focus on the Hindu Kush, Karakoram and Himalaya (HKKH) region. Though the revisit time of the PlanetScope imagery is short, courtesy of 130+ small satellites, this imagery contains only four bands and the imaging sensors in these small satellites exhibit varying spectral responses as well as lower dynamic range. Furthermore, the presence of cast shadows in the mountainous regions and varying spectral signature of the water pixels due to differences in composition, turbidity and depth makes it challenging to automatically and reliably extract surface water in PlanetScope imagery. Keeping in view these challenges, this work uses state of the art deep learning models for pixel-wise classification of PlanetScope imagery into the water and background pixels and compares the results with Random Forest and Support Vector Machine classifiers. The deep learning model is based on the popular U-Net architecture. We evaluate U-Net architecture similar to the original U-Net as well as a U-Net with a pre-trained EfficientNet backbone. In order to train the deep neural network, ground truth data are generated by manual digitization of the surface water in PlanetScope imagery with the aid of Very High Resolution Satellite (VHRS) imagery. The created dataset consists of more than 5000 water bodies having an area of approx. 71km2 in eight different sites in the HKKH region. The evaluation of the test data show that the U-Net with EfficientNet backbone achieved the highest F1 Score of 0.936. A visual comparison with the existing glacial lake inventories is then performed over the Baltoro glacier in the Karakoram range. The results show that the deep learning model detected significantly more lakes than the existing inventories, which have been derived from Landsat OLI imagery. The trained model is further evaluated on the time series PlanetScope imagery of two glacial lakes, which have resulted in an outburst flood. The output of the U-Net is also compared with the GLakeMap data. The results show that the higher spatial and temporal resolution of PlanetScope imagery is a significant advantage in the context of glacial lakes mapping and monitoring.
An Application of Sentinel-1, Sentinel-2, and GNSS Data for Landslide Susceptibility Mapping
Authors: Ghorbanzadeh Omid , Didehban Khalil , Rasouli Hamid , Kamran Khalil , Feizizadeh Bakhtiar , Blaschke Thomas
Published: September 2020

In this study, we used Sentinel-1 and Sentinel-2 data to delineate post-earthquake landslides within an object-based image analysis (OBIA). We used our resulting landslide inventory map for training the data-driven model of the frequency ratio (FR) for landslide susceptibility modelling and mapping considering eleven conditioning factors of soil type, slope angle, distance to roads, distance to rivers, rainfall, normalised difference vegetation index (NDVI), aspect, altitude, distance to faults, land cover, and lithology. A fuzzy analytic hierarchy process (FAHP) also was used for the susceptibility mapping using expert knowledge. Then, we integrated the data-driven model of the FR with the knowledge-based model of the FAHP to reduce the associated uncertainty in each approach. We validated our resulting landslide inventory map based on 30% of the global positioning system (GPS) points of an extensive field survey in the study area. The remaining 70% of the GPS points were used to validate the performance of the applied models and the resulting landslide susceptibility maps using the receiver operating characteristic (ROC) curves. Our resulting landslide inventory map got a precision of 94% and the AUCs (area under the curve) of the susceptibility maps showed 83%, 89%, and 96% for the F-AHP, FR, and the integrated model, respectively. The introduced methodology in this study can be used in the application of remote sensing data for landslide inventory and susceptibility mapping in other areas where earthquakes are considered as the main landslide-triggered factor.
PAI-1, the Plasminogen System, and Skeletal Muscle
Authors: Rahman Fasih Ahmad , Krause Matthew Paul
Published: September 2020

The plasminogen system is a critical proteolytic system responsible for the remodeling of the extracellular matrix (ECM). The master regulator of the plasminogen system, plasminogen activator inhibitor-1 (PAI-1), has been implicated for its role in exacerbating various disease states not only through the accumulation of ECM (i.e., fibrosis) but also its role in altering cell fate/behaviour. Examination of PAI-1 has extended through various tissues and cell-types with recent investigations showing its presence in skeletal muscle. In skeletal muscle, the role of this protein has been implicated throughout the regeneration process, and in skeletal muscle pathologies (muscular dystrophy, diabetes, and aging-driven pathology). Needless to say, the complete function of this protein in skeletal muscle has yet to be fully elucidated. Given the importance of skeletal muscle in maintaining overall health and quality of life, it is critical to understand the alterations—particularly in PAI-1—that occur to negatively impact this organ. Thus, we provide a comprehensive review of the importance of PAI-1 in skeletal muscle health and function. We aim to shed light on the relevance of this protein in skeletal muscle and propose potential therapeutic approaches to aid in the maintenance of skeletal muscle health.
Transcriptome-Wide Analysis of Interplay between mRNA Stability, Translation and Small RNAs in Response to Neuronal Membrane Depolarization
Authors: Kiltschewskij Dylan J. , Cairns Murray J.
Published: September 2020

Experience-dependent changes to neural circuitry are shaped by spatially-restricted activity-dependent mRNA translation. Although the complexity of mRNA translation in neuronal cells is widely appreciated, translational profiles associated with neuronal excitation remain largely uncharacterized, and the associated regulatory mechanisms are poorly understood. Here, we employed ribosome profiling, mRNA sequencing and small RNA sequencing to profile transcriptome-wide changes in mRNA translation after whole cell depolarization of differentiated neuroblast cultures, and investigate the contribution of sequence-specific regulatory mechanisms. Immediately after depolarization, a functional partition between transcriptional and translational responses was uncovered, in which many mRNAs were subjected to significant changes in abundance or ribosomal occupancy, but not both. After an extended (2 h) post-stimulus rest phase, however, these changes became synchronized, suggesting that there are different layers of post-transcriptional regulation which are temporally separated but become coordinated over time. Globally, changes in mRNA abundance and translation were found to be associated with a number of intrinsic mRNA features, including mRNA length, GC% and secondary structures; however, the effect of these factors differed between both post-depolarization time-points. Furthermore, small RNA sequencing revealed that miRNAs and tRNA-derived small RNA fragments were subjected to peak changes in expression immediately after stimulation, during which these molecules were predominantly associated with fluctuations in mRNA abundance, consistent with known regulatory mechanisms. These data suggest that excitation-associated neuronal translation is subjected to extensive temporal coordination, with substantial contributions from a number of sequence-dependent regulatory mechanisms.
The Role and Impact of Extracellular Vesicles in the Modulation and Delivery of Cytokines during Autoimmunity
Authors: Hussain Mohammed Tayab , Iqbal Asif Jilani , Norling Lucy Victoria
Published: September 2020

Cytokines and extracellular vesicles are two methods of initiating and maintaining cellular crosstalk. The role of cytokines in the initiation, progression, and resolution of inflammation has been well studied and more so, their pathophysiological role in the development of autoimmune disease. In recent years, the impact of extracellular vesicles on the progression of autoimmunity has become more widely appreciated. In this review, we discuss the mechanisms that allow extracellular vesicles of various sources to modulate cytokine production, and release, and how extracellular vesicles might be involved in the direct delivery and modulation of cytokine levels. Moreover, we explore what challenges are faced by current therapies and the promising future for extracellular vesicles as therapeutic agents in conditions driven by immune dysregulation.
Prolonged Lipid Accumulation in Cultured Primary Human Hepatocytes Rather Leads to ER Stress than Oxidative Stress
Authors: Rennert Christiane , Heil Theresa , Schicht Gerda , Stilkerich Anna , Seidemann Lena , Kegel-Hübner Victoria , Seehofer Daniel , Damm Georg
Published: September 2020

Overweight has become a major health care problem in Western societies and is accompanied by an increasing incidence and prevalence of non-alcoholic fatty liver disease (NAFLD). The progression from NAFLD to non-alcoholic steatohepatitis (NASH) marks a crucial tipping point in the progression of severe and irreversible liver diseases. This study aims to gain further insight into the molecular processes leading to the evolution from steatosis to steatohepatitis. Steatosis was induced in cultures of primary human hepatocytes by continuous five-day exposure to free fatty acids (FFAs). The kinetics of lipid accumulation, lipotoxicity, and oxidative stress were measured. Additionally, ER stress was evaluated by analyzing the protein expression profiles of its key players: PERK, IRE1a, and ATF6a. Our data revealed that hepatocytes are capable of storing enormous amounts of lipids without showing signs of lipotoxicity. Prolonged lipid accumulation did not create an imbalance in hepatocyte redox homeostasis or a reduction in antioxidative capacity. However, we observed an FFA-dependent increase in ER stress, revealing thresholds for triggering the activation of pathways associated with lipid stress, inhibition of protein translation, and apoptosis. Our study clearly showed that even severe lipid accumulation can be attenuated by cellular defenses, but regenerative capacities may be reduced.
Spectroscopic Properties of Two 5′-(4-Dimethylamino)Azobenzene Conjugated G-Quadruplex Forming Oligonucleotides
Authors: Imperatore Concetta , Varriale Antonio , Rivieccio Elisa , Pennacchio Angela , Staiano Maria , D’Auria Sabato , Casertano Marcello , Altucci Carlo , Valadan Mohammadhassan , Singh Manjot , Menna Marialuisa , Varra Michela
Published: September 2020

The synthesis of two 5′-end (4-dimethylamino)azobenzene conjugated G-quadruplex forming aptamers, the thrombin binding aptamer (TBA) and the HIV-1 integrase aptamer (T30695), was performed. Their structural behavior was investigated by means of UV, CD, fluorescence spectroscopy, and gel electrophoresis techniques in K+-containing buffers and water-ethanol blends. Particularly, we observed that the presence of the 5′-(4-dimethylamino)azobenzene moiety leads TBA to form multimers instead of the typical monomolecular chair-like G-quadruplex and almost hampers T30695 G-quadruplex monomers to dimerize. Fluorescence studies evidenced that both the conjugated G-quadruplexes possess unique fluorescence features when excited at wavelengths corresponding to the UV absorption of the conjugated moiety. Furthermore, a preliminary investigation of the trans-cis conversion of the dye incorporated at the 5′-end of TBA and T30695 showed that, unlike the free dye, in K+-containing water-ethanol-triethylamine blend the trans-to-cis conversion was almost undetectable by means of a standard UV spectrophotometer.
In Vitro Feasibility Analysis of a New Sutureless Wound-Closure System Based on a Temperature-Regulated Laser and a Transparent Collagen Membrane for Laser Tissue Soldering (LTS)
Authors: Birkelbach Moritz Alexander , Smeets Ralf , Fiedler Imke , Kluwe Lan , Wehner Martin , Trebst Tilmann , Hartjen Philip
Published: September 2020

For the post-surgical treatment of oral wounds and mucosal defects beyond a certain size, the gold standard is still an autologous skin or mucosal graft in combination with complex suturing techniques. A variety of techniques and biomaterials has been developed for sutureless wound closure including different tissue glues or collagen patches. However, no wound covering that enables for sutureless fixation has yet been introduced. Thus, a new system was developed that allows for sutureless wound covering including a transparent collagen membrane, which can be attached to the mucosa using a specially modified 2λ laser beam with integrated temperature sensors and serum albumin as bio-adhesive. The sutureless wound closure system was tested for its applicability and its cytocompatibility by an established in vitro model in the present study. The feasibility of the laser system was tested ex vivo on a porcine palate. The in vitro cytocompatibility tests excluded the potential release of toxic substances from the laser-irradiated collagen membrane and the bio-adhesive. The results of the ex vivo feasibility study using a porcine palate revealed satisfactory mean tensile strength of 1.2–1.5 N for the bonding of the membrane to the tissue fixed with laser of 980 nm. The results suggest that our newly developed laser-assisted wound closure system is a feasible approach and could be a first step on the way towards a laser based sutureless clinical application in tissue repair and oral surgery.
Effects of Diabetes Mellitus on Fibrin Clot Structure and Mechanics in a Model of Acute Neutrophil Extracellular Traps (NETs) Formation
Authors: de Vries Judith J. , Hoppenbrouwers Tamara , Martinez-Torres Cristina , Majied Rezin , Özcan Behiye , van Hoek Mandy , Leebeek Frank W.G. , Rijken Dingeman C. , Koenderink Gijsje H. , de Maat Moniek P.M.
Published: September 2020

Subjects with diabetes mellitus (DM) have an increased risk of arterial thrombosis, to which changes in clot structure and mechanics may contribute. Another contributing factor might be an increased formation of neutrophil extracellular traps (NETs) in DM. NETs are mainly formed during the acute phase of disease and form a network within the fibrin matrix, thereby influencing clot properties. Previous research has shown separate effects of NETs and DM on clot properties, therefore our aim was to study how DM affects clot properties in a model resembling an acute phase of disease with NETs formation. Clots were prepared from citrated plasma from subjects with and without DM with the addition of NETs, induced in neutrophils by S. aureus bacteria or phorbol myristate acetate (PMA). Structural parameters were measured using scanning electron microscopy, mechanical properties using rheology, and sensitivity to lysis using a fluorescence-based fibrinolysis assay. Plasma clots from subjects with DM had significantly thicker fibers and fewer pores and branch points than clots from subjects without DM. In addition, fibrinolysis was significantly slower, while mechanical properties were similar between both groups. In conclusion, in a model of acute NETs formation, DM plasma shows prothrombotic effects on fibrin clots.
Primary Cilia and Calcium Signaling Interactions
Authors: Saternos Hannah , Ley Sidney , AbouAlaiwi Wissam
Published: September 2020

The calcium ion (Ca2+) is a diverse secondary messenger with a near-ubiquitous role in a vast array of cellular processes. Cilia are present on nearly every cell type in either a motile or non-motile form; motile cilia generate fluid flow needed for a variety of biological processes, such as left–right body patterning during development, while non-motile cilia serve as the signaling powerhouses of the cell, with vital singling receptors localized to their ciliary membranes. Much of the research currently available on Ca2+-dependent cellular actions and primary cilia are tissue-specific processes. However, basic stimuli-sensing pathways, such as mechanosensation, chemosensation, and electrical sensation (electrosensation), are complex processes entangled in many intersecting pathways; an overview of proposed functions involving cilia and Ca2+ interplay will be briefly summarized here. Next, we will focus on summarizing the evidence for their interactions in basic cellular activities, including the cell cycle, cell polarity and migration, neuronal pattering, glucose-mediated insulin secretion, biliary regulation, and bone formation. Literature investigating the role of cilia and Ca2+-dependent processes at a single-cellular level appears to be scarce, though overlapping signaling pathways imply that cilia and Ca2+ interact with each other on this level in widespread and varied ways on a perpetual basis. Vastly different cellular functions across many different cell types depend on context-specific Ca2+ and cilia interactions to trigger the correct physiological responses, and abnormalities in these interactions, whether at the tissue or the single-cell level, can result in diseases known as ciliopathies; due to their clinical relevance, pathological alterations of cilia function and Ca2+ signaling will also be briefly touched upon throughout this review.
Metabolomic Analysis Reveals Changes in Preimplantation Embryos Following Fresh or Vitrified Transfer
Authors: Garcia-Dominguez Ximo , Diretto Gianfranco , Frusciante Sarah , Vicente José Salvador , Marco-Jiménez Francisco
Published: September 2020

Although assisted reproduction technologies (ARTs) are recognised as safe, and most of the offspring seem apparently healthy, there is clear evidence that ARTs are associated with changes in the embryo’s developmental trajectory, which incur physiological consequences during the prenatal and postnatal stages of life. The present study aimed to address the influence of early (day-3 embryos) embryo transfer and cryopreservation on embryo survival, size, and metabolome at the preimplantation stage (day-6 embryos). To this end, fresh-transferred (FT) and vitrified-transferred (VT) embryos were compared using naturally-conceived (NC) embryos as a control reference. The results show that as in vitro manipulation was increased (NC < FT < VT), both embryo survival rate (0.91 ± 0.02, 0.78 ± 0.05 and 0.63 ± 0.05, for NC, FT, and VT groups, respectively) and embryo size (3.21 ± 0.49 mm, 2.15 ± 0.51 mm, 1.76 ± 0.46 mm of diameter for NC, FT, and VT groups, respectively) were significantly decreased. Moreover, an unbiased metabolomics analysis showed overall down-accumulation in 40 metabolites among the three experimental groups, with embryo transfer and embryo cryopreservation procedures both exerting a cumulative effect. In this regard, targeted metabolomics findings revealed a significant reduction in some metabolites involved in metabolic pathways, such as the Krebs cycle, amino acids, unsaturated fatty acids, and arachidonic acid metabolisms. Altogether, these findings highlight a synergistic effect between the embryo transfer and vitrification procedures in preimplantation embryos. However, the ex vivo manipulation during embryo transfer seemed to be the major trigger of the embryonic changes, as the deviations added by the vitrification process were relatively smaller.