bluuebunny/crossref_metadata_2025 huggingface dataset:
Ideological Discourse Analysis of Oduduwa Secessionist Twitter Narratives
Authors: Aminu PraiseGod
Published: September 2022

The Oduduwa secessionist agitators are a group of social actors with the resolution of seceding Nigeria. Meanwhile, in spite of their reminder that Nigeria’s nationhood is still highly contested, there appears to be very little or no linguistic research on discourses produced by this emerging group of activists. Therefore, this study analyses the Oduduwa agitators’ tweets to uncover their prevailing ideologies and highlight their strategies for representing themselves and those they oppose. An analytical and qualitative research design is used to interpret the data selected. From the corpus of 10,000 tweets on Oduduwa secessionist agitators, a few tweets are purposively selected and analysed in this study. With insights from van Dijk’s model of Critical Discourse Analysis, findings reveal that Oduduwa secessionists’ Twitter posts (tweets) are protest discourses, with positive ‘we’ in-group representations and negative ‘they’ out-group constructions imprinted on them. The agitators apply linguistic strategies such as code-switching, foregrounding and hashtags to express their solidarity as well as establish social interaction. The study concludes that Oduduwa secessionist agitators’ tweets are effectively used to describe the identities of the actors, express their arguments and demands, enunciate their activities and goals, and offer information updates to the agitators and supporters.
PERAN GENERASI Z DALAM PENGUATAN EKONOMI KREATIF
Authors: Khoiriyah Ummi , Isfandayani Isfandayani , Tirtajaya Mas Deden
Published: September 2022

Creative economy is a catch in thinking to get things that are economical. However, the contribution of Generation Z to the creative economy is still small, one of which is Generation Z in Sukarukun Village. This study aims to build the role of Generation Z in strengthening the creative economy. The implementation method in this activity uses the community education method by providing socialization, training and assistance to children and adolescents related to the creative economy which aims to increase public understanding and increase awareness of the importance of Generation Z's role in strengthening the creative economy. The results of this activity show that increasing insight related to the creative economy, Generation Z can make products about the creative economy by making handicrafts in the form of pencil cases that can be of economic value, designing product photos so that they are more attractive using photoroom applications, do product packaging and marketing on social media in the form of whatsapp business, instagram and tiktok.
SOSIALISASI WEBINAR PENGENALAN OLAHRAGA CRICKET BAGI GURU SMP PJOK DI KOTA BEKASI
Authors: Fillah Ahmad Muharib , Haqiyah Aridhotul , Basri Hasan
Published: September 2022

Cricket is a sport that can be played by all ages and all groups of people, both teenagers and the general public. In general, sports have demands for achievement and there are even many competitions to sports in the village or even at a higher level. because it is a new sport in Indonesia, even though it is already a popular sport in the world. The target of this webinar activity is 30 junior high school physical education teachers in the city of Bekasi, West Java. This activity aims to introduce cricket to primary school PJOK teachers who will have the ability to form outstanding athletes. This webinar activity uses a training method that is divided into 3 stages (orientation, implementation, and evaluation), so that participants can be coordinated until the end of the event and there is an increase in teacher understanding regarding the material that has been delivered, namely history, equipment and cricket match rules.
WORKSHOP PEMBUATAN MEDIA BELAJAR POWERPOINT, MENTIMETER DAN YOUTUBE BAGI GURU SMK WIDYA NUSANTARA BEKASI
Authors: Samsiana Seta , Handayanto Rahmadya Trias , Supratno Setyo , Hasad Andi , Marini Sri , Sylviana Rika , Rakhman Taufiqur , Sujatmiko Aeri
Published: September 2022

This training provides several materials related to efforts to develop interactive media so that the learning provided becomes more communicative, increasing the interest and quality of student learning, including Video Learning Presenter View Recoder Ms. Power Point, Use of Mentimeter Interactive Media, Uploading Learning Media on Youtube. The purpose of abdimas activities is to introduce several types of learning media that can be an additional reference of knowledge in SMK Widya Nusantara Bekasi in the implementation of teaching and learning activities that are still carried out online. This is a real step from US to improve the ability of teachers to create interactive learning media independently.
GERAKAN ECOVILLAGE BERBASIS SABILULUNGAN KONSERVASI LAHAN GREENBELT WADUK JATIGEDE
Authors: Putra Purnama , Putrianika Puput , Nurhidayah Siti , Basri Hasan , Ridwan Ridwan , Widyowati Dian Desty
Published: September 2022

Jatigede Reservoir is the second largest reservoir in Indonesia. Jatigede Reservoir also has the main function for irrigation facilities and hydroelectric power (PLTA). In addition, it also functions as a means of freshwater aquaculture, water sports facilities, recreational facilities, and so on. The construction of the Jatigede Reservoir provides great benefits, but on the other hand there are sacrifices for several interests, namely the loss of community land, changes in community livelihoods and the continuity of the reservoir related to the reservoir area border with the community. Ecosystem conservation to strengthen the sustainability of the reservoir requires the concept of sabilulungan which is the local wisdom of the Sundanese people who live around the reservoir. The value of sabilulungan is not only at the level of mutual cooperation but also closely related to its relationships and interactions with others, the environment and with God. This Community Service uses the concept of Socialization and Participatory in the Sabilulungan-based Ecovillage theme in forming and maintaining the Jatigede reservoir greenbelt so that it can have an impact on social, economic, ecosystem and natural conservation aspects. The activities carried out were in the form of counseling related to the sabilulungan-based ecovillage concept, planting community forest plant seeds, health checks and distribution of basic food packages to the Cibugel village community. This activity involves village officials, community leaders, RT and RT heads, hamlet heads and other community members, in addition to expanding the scope and strengthening the level of success of the Community Service team program in collaboration with PT Migas Hulu Jabar. Community participation with village officials and community leaders is the most important thing in the sabilulungan-based Ecovillage concept, academics and CSR managers are other complementary stakeholders in maintaining the program consistency.
STRATEGI PENJUALAN MAKANAN MELALUI MEDIA DIGITAL: GOFOOD, GRABFOOD DAN SHOPEEFOOD
Authors: Halim Fajriyah Salsabila , Rahmawati Rafika , Mardiah Siti
Published: September 2022

The purpose of this community service is to help overcome the problem for MSME actors to use digital applications in developing their business as well as a means to promote through digital marketing. Various digital marketing strategies through digital applications, including Gofood, Grabfood and Shopeefood. The methodology used is descriptive qualitative with interviews, observations and socialization to MSME actors. Data is collected through documents and literature related to digital marketing and digital applications that are widely used today. This study found that digital marketing strategies have a role in promoting products. The entire digital marketing strategy, both in planning, implementing, and evaluating, has its own uniqueness. This shows that the sales strategy through digital media has been going well and provides more benefits to MSME actors in helping develop businesses, especially in the food sector by using the Gofood, Grabfood and Shopeefood applications.
STRATEGI DINAS KEPENDUDUKAN DAN PENCATATAN SIPIL DALAM MENCAPAI PELAYANAN PRIMA DI KABUPATEN BEKASI
Authors: Kunta Heriawan Abdullah , Hartono Rina Susanti
Published: September 2022

This study aims to determine the "Strategy of the Department of Population and Civil Registration in Achieving Excellent Service in Bekasi Regency" which is marked by the presence of people who complain or complain about the service that is not yet excellent. This has the potential to become a barrier to achieving these goals. This study uses a qualitative method. The data collection technique is done by means of interviews (interviews) and distributing questionnaires (questionnaires). The instrument used was a Likert scale model questionnaire, with data analysis used was categorization analysis. The sample of this study was 125 people who came to the Bekasi district civil registration and occupation service. The results of the analysis show that: (1) Based on the level of excellent service, the research subjects at Disdukcapil Bekasi regency where the attitude aspect is 62% in the good category, the attention aspect is 50% in the good category, the action aspect is 51% in the good category (Ability) 45% is in the bad category, then the appearance (appearance) is 51% in the good category and 46% is in the good category. This is based on the results of a questionnaire given to 125 research subjects. (2) Based on the conclusion of the research results, there are still obstacles in the ability aspect (ability) 45% which are categorized as not good such as slow service, limited information on service procedures, less informative service and still lack of human resources to provide services. (3) Based on the existing obstacles, Disdukcapil designs strategies to achieve excellent service such as updating administrative information, disdukcapil issues E-SIAK and makes requests through WhatsApp and adds to service human resources provided with administrative training.
IMPLEMENTASI KEBIJAKAN PERDA NOMOR 04 TAHUN 2005 DALAM UPAYA MEMFUNGSIKAN LEMBAGA PEMBERDAYAAN MASYARAKAT (LPM) DI WILAYAH KECAMATAN BEKASI UTARA KOTA BEKASI
Authors: Priatna Darma , - Sudiono
Published: September 2022

The institution is a government partner.The existence of Community Empowerment Institutions does not show its function, As an institution is only complementary, lacks role, is still weak and has not been accepted by the community, the institution is only a symbol, has not yet had an initiative, has not been able to mobilize community self- help in development.This thesis research puts forward the theory of George W. Edward III. He gave four key points in looking at the implementation of policies, namely First Communication, Both Resources, Third Disposition and the fourth Structure of Bureaucracy. The use of methods in this study using qualitative descriptive methods. In this study using a sample with a community Purposive sampling technique in North Bekasi District. Data collection techniques used in the form of observation techniques, interviews and questionnaires or questionnaires. Communication between the government and administrators of the Society and Community Empowerment Institutions has proceeded according to provisions. Resource. Already applied in the institution. according to the duties and functions of the institution. Disposition aspect in the institution's activity program, according to the trial and skills possessed. Bureaucratic structure is a series of activities according to the Standard Operating Prosedures (SOPs). Regular and conducive working mechanisms for Community Empowerment Institutions are in accordance with regional regulations and this institution can function as a forum for community participation.
IMPLEMENTASI KEBIJAKAN BADAN PENYELENGGARA JAMINAN SOSIAL (BPJS) KESEHATAN DI RUMAH SAKIT UMUM DAERAH KABUPATEN BEKASI
Authors: Suryani Ignasia , Al Rasyid M. Harun
Published: September 2022

In general, this study aims to find out how the implementation of BPJS Health policies and what factors influence the implementation of BPJS Health policies in Bekasi District Hospitals. This study used descriptive qualitative method. The data used is interviews with key informants of approximately one month. The results of the study indicate that in general the implementation of the BPJS Health policy at the Bekasi District Hospital is quite satisfactory, although there are still obstacles that must be overcome and repaired. According to the results of the study, based on the approach to the smooth implementation of routine functions and the approach to realizing the desired impact proposed by Randall B. Repley and Grace A. Franklin, several obstacles were found. Among them are the claims of BPJS Health participants at the Bekasi District Hospital that have not been paid by the BPJS Health and the revenue deficit experienced by the Obligation section.
KETERKAITAN IMPLEMENTASI ANGGARAN BERBASIS KINERJA DENGAN AKUNTABILITAS PROGRAM PRIORITAS NASIONAL KEMENTERIAN AGRARIA DAN TATA RUANG/ BADAN PERTANAHAN NASIONAL
Authors: - Suwarni , Nuraini Siti
Published: September 2022

National Priority Program policy Complete Systematic Land Registration (PTSL) in the Ministry of Agrarian and Spatial Planning / National Land Agency as an effort to realize good governance in order to accelerate the provision of legal certainty over land rights carried out in 2017-2024. The Bandung City Land Office as the implementing work unit is the only Land Office that has completed PTSL in 2017 with 152% achievement, while the August 2017 research with the results of the achievement is only 7.78% target, so that the implementation of performance-based budgeting needs to be linked with accountability. The results showed that the implementation of the implementation of performance-based budgeting at the Bandung City Land Office had implemented budget principles based on input, output and outcome with monitoring and evaluation supported by the application of the Land Program Quality Control System (SKMPP) so that it was effective and efficient. Financial accountability and performance accountability have been integrated into the Command Agency Performance Accountability System (SAKIP). The linkage between the implementation of performance-based budgets greatly influences the accountability of national priority programs. The researcher suggested to the Ministry of Agrarian and Spatial Planning / National Land Agency to make planning more optimally and the preparation of standard costs should involve work units that represent regional characteristics and conditions so that the unit of budget allocation costs in achieving performance as needed.
HUBUNGAN KEMITRAAN PEMERINTAH DAERAH DAN DEWAN PERWAKILAN RAKYAT DAERAH DALAM PENYUSUNAN APBD DI KABUPATEN INDRAMAYU
Authors: Radic Umar Sekar , Kuswandi Aos
Published: September 2022

This study identifies the partnership relationship between the Regional Government and the Regional House of Representatives (DPRD) of Indramayu Regency in the preparation of the Regional Revenue and Expenditure Budget of Indramayu Regency. The theory used is the partnership theory which contains three principles: equality, transparency and mutual benefit. The partnership theory describes how the principle of equality and the principle of transparency are implemented at the stage of preparing the Regional Revenue and Expenditure Budget in Indramayu Regency. Mutual benefit is also described as the result of the implementation of the APBD, followed by an analysis of the obstacles experienced in the preparation of the APBD in Indramayu Regency as well as the efforts of stakeholders in overcoming obstacles. The research method used is descriptive qualitative research method. The conclusion of the study shows that, there are differences in the application of the principle of equality in the musrenbang between one village/kelurahan and another in each sub-district. There is a polarization in the application of the principle of transparency among residents in terms of affiliation or political support for the Village Head (Kuwu). SKPD seems to be more dominant in the development planning process than community members. In addition, the benefits received by some community members from the implementation of the APBD every year are still relatively small.
STRATEGI KEBIJAKAN DERADIKALISASI DALAM PENANGANAN RADIKALISME PADA BADAN NASIONAL PENANGGULANGAN TERORISME (BNPT)
Authors: Restiana Viona Limfia , Susila Adi , Rofieq Ainur
Published: September 2022

Munculnya fenomena radikalisme yang mengatasnamakan agama di Indonesia menjadi perhatian serius. Ancaman teroris yang kian nyata tidak hanya dirasakan oleh Pemerintah Indonesia saja, tetapi juga dianggap sebagai ancaman global. Berulang kali Indonesia menjadi target serangan teroris, dan ada anggapan bahwa Indonesia sebagai sarang teroris. Dari Global Terrorism Index tahun 2017, diketahui bahwa Indonesia merupakan negara ke-42 di dunia yang terkena dampak terorisme. Pasca diguncang Bom Bali I dan beberapa kasus pengeboman lain, pemerintah mengeluarkan Instruksi Presiden Nomor 4 Tahun 2002 dalam rangka menanggulangi tindakan terorisme, dan pada tanggal 16 Juli 2010 Presiden Republik Indonesia dengan menerbitkan Peraturan Presiden Nomor 46 tahun 2010 tentang Badan Nasional Penanggulangan Terorisme (BNPT). Dengan dimasukkannya deradikalisasi sebagai bagian dari program atas kebijakan Perpres Nomor 46 Tahun 2010 yang merupakan bentuk strategi dari upaya pencegahan dan penanggulangan terorisme terhadap teroris, mantan teroris, dan mantan napi teroris, dan secara umum pelaku radikal anarkis dan kelompok pelaku ekstrimis yang mengatasnamakan agama. Pada perkembangannya, ternyata kasus terorisme dan radikalisme ternyata belum mampu menemukan titik hentinya. Kembalinya mantan napi teroris ke dalam jaringan teroris, memunculkan pertanyaan bagaimana program dan strategi deradikalisasi yang dilaksanakan oleh BNPT. Tulisan ini menggunakan metode kualitatif dengan pendekatan deskriptif, dengan teknik pengumpulan data yang meliputi; 1) Observasi, 2) Wawancara; dan 3) Studi Kepustakaan. Berdasarkan hasil penelitian menunjukkan bahwa terdapat strategi program deradikalisasi yang terdiri dari rehabilitas, reedukasi, resosialisasi, pembinaan wawasan kebangsaan, pembinaan keagamaan moderat, dan kewirasusahaan dengan pendekatan “Soft Approach”, yang selanjutnya diringkas ke dalam empat tahapan dalam pelaksanaan program deradikalisasi diantaranya, identifikasi, rehabilitasi, reedukasi, dan resosialisasi.
Information Control in Afghanistan, 1901–1946
Authors: Karimi Ali
Published: September 2022

This article examines the history of information control in the first half of the twentieth century in Afghanistan. This was a period of great turmoil. The world fought two devastating wars and Afghanistan went through major political and social transformations that included several violent regime changes. Despite being a neutral state, the Afghan capital attracted European rivals who campaigned for the hearts and minds of Afghans. In addition to foreign intrigues, the Afghan rulers, too, used certain information practices as part of their surveillance regimes to suppress political dissent and public unrest. A contribution to media history in Afghanistan, this article looks into how the state tried to control the flow of information in this period through surveillance, censorship, and the spread of misinformation. This was an era when print and other media technologies gained significant popularity in Afghanistan but people continued to use mostly word-of-mouth to communicate information. Despite its best efforts, which often involved brute force, the article argues, the state was not always successful in preventing people from talking with each other.
Philosophical Theories of Colour in Ancient Greek Thought – and Their Relevance Today
Authors: Sassi Maria Michela
Published: September 2022

Our modern scientific explanation of colour as a subjective impression has replaced a ‘pre-theoretical’ notion of colour as an intrinsic property of objects, which was mainstream in ancient thought. Why have we lost such pre-theoretical notion, and what have we lost by losing it? I argue that most ancient Greek philosophers exploited this pre-theoretical assumption – one that was obvious to them – in terms and ways that are still worthy of attention in the context of contemporary philosophy of colour. I offer an in-depth analysis of the theories of a number of early Greek philosophers, as well as of Plato in the Timaeus, showing that they tend to share the idea that colour is an essential property of external bodies and therefore plays a causal role in perception. Then I focus on a comparison between Democritus’ stance and Aristotle’s in order to highlight a significant contrast between the two. Democritus' theory is an exception in ancient thought because it traces the colour phenomenon back to a refraction of atmospheric light through atomic aggregates. Aristotle, instead, posits the presence of colour and light in the bodies themselves. Finally, I interpret Aristotle's account as the most complex attempt in ancient thought to weld together what we call the ‘manifest image’ of colour and its ‘scientific image’.
Spatial Variation of Soft Shell Clam Biochemical Health Status-Impacts on Age Pigments, Glucose and Melatonin Status
Authors: Gagné F.  , André C.  , Turgeon S.  , Ménard N.
Published: September 2022

Clams are sessile organisms feeding at the sediment/water interface making them particularly at risk to urban/anthropogenic pollution. The purpose of this study was therefore to examine the health impacts of wild clam populations at 2 harbors in the Saint-Lawrence Estuary and on site in Saguenay Fjord (Québec, Canada) a site known for Beluga whale high residency area. Health status was examined at tissue levels for selected metallic elements, oxidative stress, energy metabolism and circadian rhythms (serotonin and melatonin levels). The data revealed that clams collected at the fjord site (Beluga residency) were the most contaminated by Ag, Al, Cd, Cr, Co, Cu Fe, Mn, Hg, Ni Pb, and Zn. The clams were physiologically older (age related pigments) showed signs of oxidative stress (peroxidase activity and lipid peroxidation) at most of the polluted sites and were strongly correlated to most of the above elements/metals. There was an increase in glucose, esterase and ketone bodies indicative of lipid mobilization for energy. Moreover, glucose levels were significantly correlated with known hyperglycemic metals such as Cd and Pb. Based on melatonin to serotonin ratio, clams at the most polluted site in Saguenay fjord and one marina site had elevated ratio suggesting altered circadian rhythm and were seemingly out of phase to the normal tidal schedule in this area.
Microplastics and it’s Impact on Aquatic Environment
Authors: Preeti Adhana , Kalpana Singh , Vandana Garg
Published: September 2022

Microplastics (MPs), a small piece (<5mm) of plastic debris, are amongst the most serious threats to aquatic ecosystems. These are commonly found in aquatic environments due to the widespread use of plastic items. Plastic components are broken down from the large fragments into small fragments during the treatment procedure in treatment plants of wastewater, these plants can operate as the entry points for the MPs into the aquatic ecosystem; so it is necessary that MPs must be removed from the wastewater during the treatment process. However, there is not sufficient data available about MPs’ impact on the ecological services cascade and how it is linked with the declining biodiversity. This review examines the outcomes of MPs in the aquatic environment, their role as carriers, and the possible influence of MPs on aquatic biota. In this review detailed overview of existing knowledge regarding MP aggregation in the aquatic ecosystem is provided.
Burrow Morphology of Brachyuran Crab Dotilla blanfordi Alcock, 1900 from Gulf of Khambhat, Gujarat, India
Authors: Kanaiyalal S. Upadhyay , Krupal J. Patel , Jayesh M. Prajapati , Vasantkumar M. Rabari , Dimple R. Thacker , Heris V. Patel , Jigneshkumar N. Trivedi
Published: September 2022

The present study was conducted to understand the burrow morphology of brachyuran crab Dotilla blanfordi Alcock, 1900 at Dandi beach located in Gulf of Khambhat of Gujarat state, India. Burrows were selected randomly and their opening diameter was recorded. Diluted resin was poured in the burrows to generate burrow cast. If the occupant crab emerges out of the burrow, it was captured and carapace width and gender was recorded. The shapes of complete burrow casts were identified and measurements of various morphological characters of burrow cast were recorded. Total 7 different burrow shapes were identified, out of which single tube burrow was the most common as compared to other burrow shapes. The crab carapace width showed significant relationship with burrow opening diameter, burrow depth, burrow length and burrow volume. Juveniles (3.45 mm CW) were making burrow of all the seven shapes. Burrow depth, length and volume were significantly higher in adult crabs as compared to juveniles. No significant variation was observed between burrows of male and female individuals. The present study showed that burrow morphology is highly affected by the crab body size and also varies significantly with different life stages of the crab. Juveniles make shallow and simple burrows as their smaller gill areas stores lesser amount of respiratory water which needs to be changed frequently. However, adults make complex burrows to perform various tasks including copulation and reproduction.
Regenerative medicine technologies applied to transplant medicine. An update
Authors: Petrosyan Astgik , Montali Filippo , Peloso Andrea , Citro Antonio , Byers Lori N. , La Pointe Catherine , Suleiman Mara , Marchetti Alice , Mcneill Eoin P. , Speer Allison L , Ng Wai Hoe , Ren Xi , Bussolati Benedetta , Perin Laura , Di Nardo Paolo , Cardinale Vincenzo , Duisit Jerome , Monetti Alexandra Rose , Savino John Richard , Asthana Amish , Orlando Giuseppe
Published: September 2022

Regenerative medicine (RM) is changing how we think and practice transplant medicine. In regenerative medicine, the aim is to develop and employ methods to regenerate, restore or replace damaged/diseased tissues or organs. Regenerative medicine investigates using tools such as novel technologies or techniques, extracellular vesicles, cell-based therapies, and tissue-engineered constructs to design effective patient-specific treatments. This review illustrates current advancements in regenerative medicine that may pertain to transplant medicine. We highlight progress made and various tools designed and employed specifically for each tissue or organ, such as the kidney, heart, liver, lung, vasculature, gastrointestinal tract, and pancreas. By combing both fields of transplant and regenerative medicine, we can harbor a successful collaboration that would be beneficial and efficacious for the repair and design of de novo engineered whole organs for transplantations.
Soft-tissue simulation of the breast for intraoperative navigation and fusion of preoperative planning
Authors: Alcañiz Patricia , Vivo de Catarina César , Gutiérrez Alessandro , Pérez Jesús , Illana Carlos , Pinar Beatriz , Otaduy Miguel A.
Published: September 2022

Computational preoperative planning offers the opportunity to reduce surgery time and patient risk. However, on soft tissues such as the breast, deviations between the preoperative and intraoperative settings largely limit the applicability of preoperative planning. In this work, we propose a high-performance accurate simulation model of the breast, to fuse preoperative information with the intraoperative deformation setting. Our simulation method encompasses three major elements: high-quality finite-element modeling (FEM), efficient handling of anatomical couplings for high-performance computation, and personalized parameter estimation from surface scans. We show the applicability of our method on two problems: 1) transforming high-quality preoperative scans to the intraoperative setting for fusion of preoperative planning data, and 2) real-time tracking of breast tumors for navigation during intraoperative radiotherapy. We have validated our methodology on a test cohort of nine patients who underwent tumor resection surgery and intraoperative radiotherapy, and we have quantitatively compared simulation results to intraoperative scans. The accuracy of our simulation results suggest clinical viability of the proposed methodology.
Life cycle energy analysis of residential wooden buildings versus concrete and steel buildings: A review
Authors: Schenk Daniela , Amiri Ali
Published: September 2022

Around 40% of global energy consumption can be attributed to the construction sector. Consequently, the development of the construction industry towards more sustainable solutions and technologies plays a crucial role in the future of our planet. Various tools and methods have been developed to assess the energy consumption of buildings, one of which is life cycle energy analysis (LCEA). LCEA requires the energy consumption at each stage of the life cycle of a product to be assessed, enabling the comparison of the impact of construction materials on energy consumption. Findings from LCEAs of buildings suggest that timber framed constructions show promising results with respect to energy consumption and sustainability. In this study a critical analysis of 100 case studies from the literature of LCEAs conducted for residential buildings is presented. Based on the studied material, the embodied, operational, and demolition energies for timber, concrete and steel buildings are compared and the importance of sustainable material selection for buildings is highlighted. The results reveal that on average, the embodied energy of timber buildings is 28–47% lower than for concrete and steel buildings respectively. The mean and median values of embodied emissions are 2,92 and 2,97 for timber, 4.08 and 3,95 for concrete, and 5,55 and 5,53 GJ/m2 for steel buildings. Moreover, the data suggests that the energy supply system of residential buildings plays a larger role in the operational energy consumption that the construction material. In addition, climate conditions, insulation detail, windows and building surfaces, and building direction are the other energy use role players. Finally, it was found that the demolition energy contributes only a small amount to the total life cycle energy consumption. This study demonstrates the significance of embodied energy when comparing the life cycle energy requirements of buildings and highlights the need for the development of a more standardised approach to LCEA case studies.
Egr1 plays a major role in the transcriptional response of white adipocytes to insulin and environmental cues
Authors: Meriin A. B. , Zaarur N. , Roy D. , Kandror K. V.
Published: September 2022

It is believed that insulin regulates metabolic functions of white adipose tissue primarily at the post-translational level via the PI3K-Akt-mediated pathway. Still, changes in transcription also play an important role in the response of white adipocytes to insulin and environmental signals. One transcription factor that is dramatically and rapidly induced in adipocytes by insulin and nutrients is called Early Growth Response 1, or Egr1. Among other functions, it directly binds to promoters of leptin and ATGL stimulating the former and inhibiting the latter. Furthermore, expression of Egr1 in adipocytes demonstrates cell autonomous circadian pattern suggesting that Egr1 not only mediates the effect of insulin and nutrients on lipolysis and leptin production but also, coordinates insulin action with endogenous circadian rhythms of adipose tissue.
Isolation of Viable Single Cells With High Yield and Purity Using a Small Amount of Human Kidney Tissue Biopsy
Authors: Yaigoub Hasnaa , Tirichen Hasna , Xin Xiaohong , Shi Shuhong , Wu Changxin , Li Rongshan , Li Yafeng
Published: September 2022

Objective: Establishment of an efficient method of preparing human kidney single cell suspension, using a very small amount of tissue puncture.

Methods: Samples of human kidney tissue puncture were cut into pieces, and then 80 μL of the digestive enzyme were added to each punctured tissue to induce enzymatic digestion. The enzyme combination is composed of collagenases, DNase and hyaluronidase and the sample was incubated 20 min at 37°C. The obtained cell suspension was filtered through a 70 μm cell strainer, centrifuged at 300 g for 5 min and the supernatant was removed, then the pellet was resuspended in 3 ml of DMEM (Dulbecco’s Modified Eagle’s Medium). Cell suspension was sorted and purified by flow sorting to remove dead cells and obtain a cell suspension with higher viability rate.

Results: We found that 1) diverse single cells of human kidney can be obtained by the digestive enzyme, as observed under the light microscope, with different sizes, normal cell morphology and good dispersion. 2) (2-3) × 106 single cells can be extracted from one fresh punctured kidney tissue of about 10 mg, with a cell viability rate of more than 80%.

Conclusion: In this work we generated a comprehensive and high-resolution single-cell method, which is simple and efficient for preparing single cell suspension from a minimal amount of human kidney tissue. This method can facilitate the study of renal cell biology and the pathogenesis of kidney diseases.
The MASTL-ENSA-PP2A/B55 axis modulates cisplatin resistance in oral squamous cell carcinoma
Authors: Gouttia Odjo G. , Zhao Jing , Li Yanqiu , Zwiener Mackenzie J. , Wang Ling , Oakley Gregory G. , Peng Aimin
Published: September 2022

Platinum-based chemotherapy is the standard first-line treatment for oral squamous cell carcinoma (OSCC) that is inoperable, recurrent, or metastatic. Platinum sensitivity is a major determinant of patient survival in advanced OSCC. Here, we investigated the involvement of MASTL, a cell cycle kinase that mediates ENSA/ARPP19 phosphorylation and PP2A/B55 inhibition, in OSCC therapy. Interestingly, upregulation of MASTL and ENSA/ARPP19, and downregulation of PP2A/B55, were common in OSCC. MASTL expression was in association with poor patient survival. In established OSCC cell lines, upregulation of MASTL and ENSA, and downregulation of B55 genes, correlated with cisplatin resistance. We further confirmed that stable expression of MASTL in OSCC cells promoted cell survival and proliferation under cisplatin treatment, in an ENSA-dependent manner. Conversely, deletion of MASTL or ENSA, or overexpression of B55α, sensitized cisplatin response, consistent with increased DNA damage accumulation, signaling, and caspase activation. Moreover, GKI-1, the first-in-class small molecule inhibitor of MASTL kinase, phenocopied MASTL depletion in enhancing the outcome of cisplatin treatment in OSCC cells, at a dose substantially lower than that needed to disrupt mitotic entry. Finally, GKI-1 exhibited promising efficacy in a mouse tumor xenograft model, in conjunction with cisplatin therapy.
Cryptotanshinone inhibits ovarian tumor growth and metastasis by degrading c-Myc and attenuating the FAK signaling pathway
Authors: Guo Huijun , Zhang Wenjing , Wang Jiaxing , Zhao Guannan , Wang Yaohong , Zhu Bing-Mei , Dong Peixin , Watari Hidemichi , Wang Baojin , Li Wei , Tigyi Gabor , Yue Junming
Published: September 2022

Cryptotanshinone (CT), a natural compound derived from Salvia miltiorrhiza Bunge that is also known as the traditional Chinese medicine Danshen, exhibits antitumor activity in various cancers. However, it remains unclear whether CT has a potential therapeutic benefit against ovarian cancers. The aim of this study was to test the efficacy of CT in ovarian cancer cells in vitro and using a xenograft model in NSG mice orthotopically implanted with HEY A8 human ovarian cancer cells and to explore the molecular mechanism(s) underlying CT’s antitumor effects. We found that CT inhibited the proliferation, migration, and invasion of OVCAR3 and HEY A8 cells, while sensitizing the cell responses to the chemotherapy drugs paclitaxel and cisplatin. CT also suppressed ovarian tumor growth and metastasis in immunocompromised mice orthotopically inoculated with HEY A8 cells. Mechanistically, CT degraded the protein encoded by the oncogene c-Myc by promoting its ubiquitination and disrupting the interaction with its partner protein Max. CT also attenuated signaling via the nuclear focal adhesion kinase (FAK) pathway and degraded FAK protein in both cell lines. Knockdown of c-Myc using lentiviral CRISPR/Cas9 nickase resulted in reduction of FAK expression, which phenocopies the effects of CT and the c-Myc/Max inhibitor 10058-F4. Taken together, our studies demonstrate that CT inhibits primary ovarian tumor growth and metastasis by degrading c-Myc and FAK and attenuating the FAK signaling pathway.
Electrochemically induced in vitro focal hypoxia in human neurons
Authors: Wong Joseph J. Y. , Varga Balazs V. , Káradóttir Ragnhildur Thóra , Hall Elizabeth A. H.
Published: September 2022

Focalised hypoxia is widely prevalent in diseases such as stroke, cardiac arrest, and dementia. While in some cases hypoxia improves cellular functions, it mostly induces or exacerbates pathological changes. The lack of methodologies that can simulate focal acute hypoxia, in either animal or cell culture, impedes our understanding of the cellular consequences of hypoxia. To address this gap, an electrochemical localised oxygen scavenging system (eLOS), is reported, providing an innovative platform for spatiotemporal in vitro hypoxia modulation. The electrochemical system is modelled showing O2 flux patterns and localised O2 scavenging and hypoxia regions, as a function of distance from the electrode and surrounding flux barriers, allowing an effective focal hypoxia tool to be designed for in vitro cell culture study. O2 concentration is reduced in an electrochemically defined targeted area from normoxia to hypoxia in about 6 min depending on the O2-flux boundaries. As a result, a cell culture-well was designed, where localised O2 scavenging could be induced. The impact of localised hypoxia was demonstrated on human neural progenitor cells (hNPCs) and it was shown that miniature focal hypoxic insults can be induced, that evoke time-dependent HIF-1α transcription factor accumulation. This transcription is “patterned” across the culture according to the electrochemically induced spatiotemporal hypoxia gradient. A basic lacunar infarct model was also developed through the application of eLOS in a purpose designed microfluidic device. Miniature focal hypoxic insults were induced in cellular processes of fully oxygenated cell bodies, such as the axons of human cortical neurons. The results demonstrate experimentally that localised axonal hypoxic stress can lead to significant increase of neuronal death, despite the neurons remaining at normoxia. This suggests that focal hypoxic insult to axons alone is sufficient to impact surrounding neurons and may provide an in vitro model to study the impact of microinfarcts occurring in the deep cerebral white matter, as well as providing a promising tool for wider understanding of acute hypoxic insults with potential to uncover its pathophysiology in multiple diseases.
Novel inhibitors and activity-based probes targeting serine proteases
Authors: Ferguson Timothy E. G. , Reihill James A. , Martin S. Lorraine , Walker Brian
Published: September 2022

Serine proteases play varied and manifold roles in important biological, physiological, and pathological processes. These include viral, bacterial, and parasitic infection, allergic sensitization, tumor invasion, and metastasis. The use of activity-based profiling has been foundational in pinpointing the precise roles of serine proteases across this myriad of processes. A broad range of serine protease-targeted activity-based probe (ABP) chemotypes have been developed and we have recently introduced biotinylated and “clickable” peptides containing P1N-alkyl glycine arginineN-hydroxy succinimidyl (NHS) carbamates as ABPs for detection/profiling of trypsin-like serine proteases. This present study provides synthetic details for the preparation of additional examples of this ABP chemotype, which function as potent irreversible inhibitors of their respective target serine protease. We describe their use for the activity-based profiling of a broad range of serine proteases including trypsin, the trypsin-like protease plasmin, chymotrypsin, cathepsin G, and neutrophil elastase (NE), including the profiling of the latter protease in clinical samples obtained from patients with cystic fibrosis.
Clinical study on sequential treatment of severe diarrhea irritable bowel syndrome with precision probiotic strains transplantation capsules, fecal microbiota transplantation capsules and live combined bacillus subtilis and enterococcus faecium capsules
Authors: Lv Linling , Ruan Guangcong , Ping Yi , Cheng Yi , Tian Yuting , Xiao Zhifeng , Zhao Xuefei , Chen Dongfeng , Wei Yanling
Published: September 2022

Objective

To study the effect of precision probiotic strains transplantation capsules on diarrhea irritable bowel syndrome compared with fecal microbiota transplantation capsules and live combined bacillus subtilis and enterococcus faecium capsules.

Methods

Two patients with severe irritable bowel syndrome were treated with precision probiotic strains transplantation capsules, fecal microbiota transplantation capsules and live combined bacillus subtilis and enterococcus faecium capsules in sequence. IBS-SSS, IBS-QoL, GSRS, stool frequency, stool character, degree of abdominal pain, GAD-7, and PHQ9 scores of patients at 0, 2, 4, 6, 8, 10, and 12 weeks of treatment were monitored and recorded, and stool samples were collected for metagenomics and metabolomics.

Results

It was found that the IBS-SSS score of patient case 1 decreased by 175 points and that of patient case 2 decreased by 100 points after treatment of precision probiotic strains transplantation capsules. There was no significant decrease after fecal microbiota transplantation capsules and live combined bacillus subtilis and enterococcus faecium capsules were used. At the same time, compared with fecal microbiota transplantation and live combined bacillus subtilis and enterococcus faecium capsules, the IBS QoL, stool frequency, stool character, degree of abdominal pain and GAD-7 score of patient case 1 improved more significantly by the precision probiotic strains transplantation capsules. And the stool frequency and stool character score of patient case 2 decreased more significantly. Intestinal microbiota also improved more significantly after the precise capsule transplantation treatment. And we found Eubacterium_ Eligens showed the same change trend in the treatment of two patients, which may play a role in the treatment.

Conclusion

precision probiotic strains transplantation capsules is more beneficial to improve the intestinal microbiota of patients than microbiota transplantation capsule and live combined bacillus subtilis and enterococcus faecium capsules, so as to better alleviate clinical symptoms. This study provides a more perfect and convenient therapeutic drugs for the treatment of IBS.
The impact of UEFA Euro 2020 football championship on Takotsubo Syndrome: Results of a multicenter national registry
Authors: Polimeni Alberto , Spaccarotella Carmen , Ielapi Jessica , Esposito Giovanni , Ravera Amelia , Martuscelli Eugenio , Ciconte Vincenzo , Menichelli Maurizio , Varbella Ferdinando , Imazio Massimo , Navazio Alessandro , Sinagra Gianfranco , Oberhollenzer Rainer , Sibilio Gerolamo , Cacciavillani Luisa , Meloni Luigi , Dominici Marcello , Tomai Fabrizio , Amico Francesco , Corda Marco , Musumeci Giuseppe , Lupi Alessandro , Zezza Luigi , De Caterina Raffaele , Cernetti Carlo , Metra Marco , Rossi Lidia , Calabrò Paolo , Murrone Adriano , Volpe Massimo , Caldarola Pasquale , Carugo Stefano , Cortese Bernardo , Valenti Renato , Boriani Giuseppe , Fedele Francesco , Ventura Giorgio , Manes Maria Teresa , Colavita Angela Rita , Feola Mauro , Versaci Francesco , Assennato Pasquale , Arena Giuseppe , Ceravolo Roberto , Amodeo Vincenzo , Tortorici Gianfranco , Nassiacos Daniele , Antonicelli Roberto , Esposito Nicolino , Favale Stefano , Licciardello Giovanni , Tedesco Luigi , Indolfi Ciro
Published: September 2022

Objectives

The UEFA 2020 European Football Championship held in multiple cities across Europe from June 11 to July 11, 2021, was won by Italy, providing an opportunity to examine the relationship between emotional stress and the incidence of acute cardiovascular events (ACE).

Methods and results

Cardiovascular hospitalizations in the Cardiac Care Units of 49 hospital networks in Italy were assessed by emergency physicians during the UEFA Euro 2020 Football Championship. We compared the events that occurred during matches involving Italy with events that occurred during the remaining days of the championship as the control period. ACE was assessed in 1,235 patients. ACE during the UEFA Euro 2020 Football Championship semifinal and final, the most stressful matches ended with penalties and victory of the Italian team, were assessed. A significant increase in the incidence of Takotsubo Syndrome (TTS) by a factor of 11.41 (1.6–495.1, P < 0.003), as compared with the control period, was demonstrated during the semifinal and final, whereas no differences were found in the incidence of ACS [IRR 0.93(0.74–1.18), P = 0.57]. No differences in the incidence of ACS [IRR 0.98 (0.87–1.11; P = 0.80)] or TTS [IRR 1.66(0.80–3.4), P = 0.14] were found in the entire period including all matches of the UEFA Euro 2020 compared to the control period.

Conclusions

The data of this national registry demonstrated an association between the semifinal and final of UEFA Euro 2020 and TTS suggesting that it can be triggered by also positive emotions such as the victory in the European Football Championship finals.
Weekly earthquake prediction in a region of China based on an intensive precursor network AETA
Authors: Xie Jinhan , Yong Shanshan , Wang Xin’an , Bao Zhenyu , Liu Yibin , Zhang Xing , He Chunjiu
Published: September 2022

Once a majority of earthquakes occur without prediction, it is very likely to have a huge impact on human society. To solve the worldwide challenging problem of earthquake prediction, our laboratory has developed a set of sensory systems to monitor the abnormal activity of geological signals before an earthquake happens in China. At present, more than 300 stations have been deployed, and the observation time has exceeded 4 years. Based on the various geological activities collected, a local correlation tracking method is used to capture signal anomalies before an earthquake, and then the ROC curve is used for the evaluation of the predictive accuracy. The method is applied in the Sichuan-Yunnan area weekly, verifying the forecast within a 91-week time frame and a 30-week time frame. The method proposed in this article has earthquake prediction ability with a rate of over 70%. It promotes and contributes to helping people avoid the fear of unpredictable earthquakes.