bluuebunny/crossref_metadata_2025 huggingface dataset:
The acoustic advantage of hunting at low heights above water: behavioural experiments on the European ‘trawling’ bats Myotis capaccinii, M. dasycneme and M. daubentonii
Authors: Siemers Björn M. , Stilz Peter , Schnitzler Hans-Ulrich
Published: April 2021

We have demonstrated in behavioural experiments that success in capturing prey from surfaces in ‘trawling Myotis’ (Leuconoë-type) depends on the acoustic properties of the surface on which the prey is presented. Two types of surface structure were ensonified with artificial bat signals to probe their acoustic characteristics. We have shown that perception of prey by echolocation is easier if the prey is presented on a smooth surface (such as calm water) than if it is presented on a structured surface (such as vegetation or the ground). This is because the smooth surface reflects a much lower level of clutter echoes than the structured one if ensonified at an angle typical for bats foraging low over water. The ensonification experiments revealed that the sound pressure level of the echo was even higher for mealworms on a smooth surface than for mealworms suspended in air. This might be because waves travelling via the surface also contribute to the echo (e.g. reflection from the surface to the mealworm, back to the surface and then to the receiver). From the behavioural experiments, we conclude that ‘trawling Myotis’ take isolated objects on smooth (water) surfaces for prey. Those objects reflect isolated, stationary acoustic glints back to the echolocating bats. Conversely, ‘trawling Myotis’ will not recognise prey if prey echoes are embedded in numerous clutter echoes. We have demonstrated marked similarities between the three European ‘trawling Myotis’ species M. dasycneme, M. daubentonii and M. capaccinii in echolocation behaviour, search image, foraging strategy and prey perception. We propose that a combination of prey abundance and acoustic advantages could have led to repeated and convergent evolution of ‘trawling’ bats in different parts of the world.
Migrating songbirds tested in computer-controlled Emlen funnels use stellar cues for a time-independent compass
Authors: Mouritsen Henrik , Larsen Ole Næsbye
Published: April 2021

This paper investigates how young pied flycatchers, Ficedula hypoleuca, and blackcaps, Sylvia atricapilla, interpret and use celestial cues. In order to record these data, we developed a computer-controlled version of the Emlen funnel, which enabled us to make detailed temporal analyses. First, we showed that the birds use a star compass. Then, we tested the birds under a stationary planetarium sky, which simulated the star pattern of the local sky at 02:35 h for 11 consecutive hours of the night, and compared the birds’ directional choices as a function of time with the predictions from five alternative stellar orientation hypotheses. The results supported the hypothesis suggesting that birds use a time-independent star compass based on learned geometrical star configurations to pinpoint the rotational point of the starry sky (north). In contrast, neither hypotheses suggesting that birds use the stars for establishing their global position and then perform true star navigation nor those suggesting the use of a time-compensated star compass were supported.
Effects of training on forced submersion responses in harbor seals
Authors: Jobsis P. D. , Ponganis P. J. , Kooyman G. L.
Published: April 2021

In several pinniped species, the heart rates observed during unrestrained dives are frequently higher than the severe bradycardias recorded during forced submersions. To examine other physiological components of the classic ‘dive response’ during such moderate bradycardias, a training protocol was developed to habituate harbor seals (Phoca vitulina) to short forced submersions. Significant changes were observed between physiological measurements made during naive and trained submersions (3–3.5 min). Differences were found in measurements of heart rate during submersion (naive 18±4.3 beats min–1versus trained 35±3.4 beats min–1), muscle blood flow measured using laser-Doppler flowmetry (naive 1.8±0.8 ml min–1 100 g–1versus trained 5.8±3.9 ml min–1 100 g–1), change in venous PO2 (naive –0.44±1.25 kPa versus trained –1.48±0.76 kPa) and muscle deoxygenation rate (naive –0.67±0.27 mvd s–1versus trained –0.51±0.18 mvd s–1, a relative measure of muscle oxygenation provided by the Vander Niroscope, where mvd are milli-vander units). In contrast to the naive situation, the post-submersion increase in plasma lactate levels was only rarely significant in trained seals. Resting eupneic (while breathing) heart rate and total oxygen consumption rates (measured in two seals) were not significantly different between the naive and trained states. This training protocol revealed that the higher heart rate and greater muscle blood flow in the trained seals were associated with a lower muscle deoxygenation rate, presumably secondary to greater extraction of blood O2 during trained submersions. Supplementation of muscle oxygenation by blood O2 delivery during diving would increase the rate of blood O2 depletion but could prolong the duration of aerobic muscle metabolism during diving. This alteration of the dive response may increase the metabolic efficiency of diving.
Regulation of intracellular pH in anoxia-tolerant and anoxia-intolerant teleost hepatocytes
Authors: Krumschnabel Gerhard , Manzl Claudia , Schwarzbaum Pablo J.
Published: April 2021

Mechanisms of intracellular pH (pHi) regulation were investigated in anoxia-tolerant hepatocytes from goldfish Carassius auratus, and compared to the situation in the anoxia-intolerant hepatocytes from trout Oncorhynchus mykiss. Under normoxic conditions, the pHi of goldfish hepatocytes was regulated by a Na+/H+ exchanger and a Na+-independent Cl–/HCO3– exchanger, the latter being activated only after acidification of the cells. Mechanisms of acid secretion appear to be fuelled, at least in part, by lactate formation under fully aerobic conditions, as inhibition of glycolysis caused a drastic reduction of steady state proton release. In trout hepatocytes both a Na+/H+ exchanger and a Cl–/HCO3– exchanger were found to be tonically active, as described previously. During chemical anoxia a constant pHi was maintained in goldfish hepatocytes, whereas it was reversibly reduced by 0.3 units in the trout cells. Under these conditions a reversible increase in the rate of acid secretion was induced in the cells from both species. In the goldfish cells this was based on a SITS-sensitive transporter, possibly involving export of lactate, with no contribution from Na+/H+ exchange. By contrast, in hepatocytes from trout, CN-induced acid secretion was dominated by the activity of the Na+/H+ exchanger. Brief exposure to extracellular acidosis had no dramatic effects on the energetics of hepatocytes from either species.
Evidence for a mineralocorticoid-like receptor linked to branchial chloride cell proliferation in freshwater rainbow trout
Authors: Sloman Katherine A. , Desforges Patrick R. , Gilmour Kathleen M.
Published: April 2021

Fish acclimated to ion-deficient water exhibit proliferation of branchial chloride cells. The objective of the present study was to investigate the role of cortisol in this response using the corticosteroid receptor antagonists RU486 and spironolactone. RU486 is a potent antagonist of the glucocorticoid actions of cortisol, whereas spironolactone exhibits high-affinity binding to mineralocorticoid receptors, with a resulting blockade of mineralocorticoid properties in mammals. Untreated rainbow trout, as well as rainbow trout given a single intraperitoneal implant of coconut oil alone, coconut oil containing RU486 (0.5 mg g–1) or coconut oil containing spironolactone (0.1 mg g–1), were exposed to either dechlorinated city-of-Ottawa tapwater or artificial softwater for 7 days. Neither corticosteroid antagonist nor acclimation condition affected circulating plasma cortisol levels, plasma ion concentrations or gill Na+-K+-ATPase activity. Kidney Na+-K+-ATPase activity was significantly higher in softwater-acclimated fish than in fish held in dechlorinated tapwater. In addition, whereas RU486 treatment was found to be without effect on gill morphometrics, treatment with spironolactone inhibited the proliferation of chloride cells normally associated with acclimation to ion-deficient water. The results of the present study provide further evidence for the mineralocorticoid actions of cortisol in freshwater fish, specifically in eliciting chloride cell proliferation. Furthermore, these results support the hypothesis that distinct glucocorticoid and mineralocorticoid receptor populations are present in teleost fish, despite the apparent absence of the classic mineralocorticoid hormone, aldosterone.
Na++K+-ATPase in gills of the blue crabCallinectes sapidus: cDNA sequencing and salinity-related expression of α-subunit mRNA and protein
Authors: Towle David W. , Paulsen Ryan S. , Weihrauch Dirk , Kordylewski Marek , Salvador Cristina , Lignot Jean-Hervé , Spanings-Pierrot Céline
Published: April 2021

Many studies have shown that hyperosmoregulation in euryhaline crabs is accompanied by enhanced Na++K+-ATPase activity in the posterior gills, but it remains unclear whether the response is due to regulation of pre-existing enzyme or to increased gene transcription and mRNA translation. To address this question, the complete open reading frame and 3′ and 5′ untranslated regions of the mRNA coding for the α-subunit of Na++K+-ATPase from the blue crab Callinectes sapidus were amplified by reverse transcriptase/polymerase chain reaction (RT-PCR) and sequenced. The resulting 3828-nucleotide cDNA encodes a putative 1039-amino-acid protein with a predicted molecular mass of 115.6 kDa. Hydrophobicity analysis of the amino acid sequence indicated eight membrane-spanning regions, in agreement with previously suggested topologies. The α-subunit amino acid sequence is highly conserved among species, with the blue crab sequence showing 81–83 % identity to those of other arthropods and 74–77 % identity to those of vertebrate species. Quantitative RT-PCR analysis showed high levels of α-subunit mRNA in posterior gills 6–8 compared with anterior gills 3–5. Western blots of gill plasma membranes revealed a single Na++K+-ATPase α-subunit protein band of the expected size. The posterior gills contained a much higher level of α-subunit protein than the anterior gills, in agreement with previous measurements of enzyme activity. Immunocytochemical analysis showed that the Na++K+-ATPase α-subunit protein detected by α5 antibody is localized to the basolateral membrane region of gill epithelial cells. Transfer of blue crabs from 35 to 5 ‰ salinity was not accompanied by notable differences in the relative proportions of α-subunit mRNA and protein in the posterior gills, suggesting that the enhanced Na++K+-ATPase enzyme activity that accompanies the hyperosmoregulatory response may result from post-translational regulatory processes.
The effects of depth on the cardiac and behavioural responses of double-crested cormorants (Phalacrocorax auritus) during voluntary diving
Authors: Enstipp Manfred R. , Andrews Russel D. , Jones David R.
Published: April 2021

Heart rate and dive behaviour were monitored in double-crested cormorants (Phalacrocorax auritus) during shallow (1 m) and deep diving (12 m), after breathing different gas mixtures, to investigate the role of depth and the accompanying changes in blood gas levels in cardiac and behavioural control during voluntary diving. Pre-dive heart rate in both shallow- and deep-diving birds was approximately three times the resting heart rate (137.9±17.5 beats min–1; mean ± s.d., N=5), falling abruptly upon submersion to around 200–250 beats min–1. During shallow diving, the initial reduction in heart rate was followed by a secondary, more gradual decline, to around the resting level. In contrast, during deep diving, heart rate stabilised at 200–250 beats min–1. In dives of similar duration, mean dive heart rate was significantly lower during shallow diving (163.2±14.0 beats min–1) than during deep diving (216.4±7.7 beats min–1), but in both cases was significantly above the resting value. The difference in cardiac response is probably due to an increase in arterial oxygen tension (PaO2) during the descent phase of deep dives (compression hyperoxia). Exposure to a hyperoxic gas mixture before shallow diving significantly increased mean dive heart rate, while exposure to a hypoxic gas mixture in both the shallow and deep dive tanks significantly reduced mean dive heart rate. In contrast, breathing hypercapnic gas before diving had no significant effect on dive heart rate. We suggest that the cardiac response to voluntary diving in double-crested cormorants is strongly influenced by changes in blood oxygen levels throughout the dive. Dive duration was unaffected by alterations in inspired gas composition, but surface interval duration decreased during hyperoxic gas exposure and increased during hypoxic gas exposure. The most efficient dive pattern (highest dive/pause ratio) was observed after hyperoxic exposure. Our study suggests that blood oxygen level is a powerful stimulus that facilitates the cardiac and behavioural adjustments during foraging that are important components of a strategy allowing double-crested cormorants to maximise the time spent under water and, hence, potential foraging time.
The role of angiotensin II in regulating catecholamine secretion during hypoxia in rainbow troutOncorhynchus mykiss
Authors: Lapner Katherine N. , Perry Steve F.
Published: April 2021

Experiments were performed in vivo on chronically cannulated adult rainbow trout (Oncorhynchus mykiss) to assess the involvement of serotonergic or muscarinic receptor stimulation or activation of the renin–angiotensin system in eliciting catecholamine release during acute hypoxia during periods of nicotinic receptor desensitisation.Despite nicotinic receptor desensitisation induced by intravenous infusion of nicotine (1.3×10–5 mol kg–1 h–1), plasma catecholamine levels were increased to levels (adrenaline plus noradrenaline 125–200 nmol l–1) similar to those in control fish during severe hypoxia (40–45 mmHg; 5.3–6.0 kPa). Blockade of serotonergic receptors using methysergide or of muscarinic receptors using atropine did not affect the ability of fish to elevate circulating catecholamine levels during hypoxia. However, selective blockade of the renin–angiotensin system, using lisinopril to inhibit angiotensin-converting enzyme, prevented the elevation of both angiotensin II and circulating catecholamine levels in acutely hypoxic fish experiencing nicotinic receptor desensitisation. In fish possessing functional nicotinic receptors, angiotensin-converting enzyme blockade attenuated but did not prevent the elevation of plasma catecholamine levels during hypoxia. The results of this study indicate that the renin–angiotensin system is activated during hypoxia and plays a role in eliciting catecholamine release that is secondary to activation of nicotinic receptors. However, under conditions of nicotinic receptor desensitisation, activation of the renin–angiotensin system during hypoxia is a prerequisite for catecholamine release.
The correlation between wing kinematics and steering muscle activity in the blowfly Calliphora vicina
Authors: Balint Claire N. , Dickinson Michael H.
Published: April 2021

Determining how the motor patterns of the nervous system are converted into the mechanical and behavioral output of the body is a central goal in the study of locomotion. In the case of dipteran flight, a population of small steering muscles controls many of the subtle changes in wing kinematics that allow flies to maneuver rapidly. We filmed the wing motion of tethered Calliphora vicina at high speed and simultaneously recorded multi-channel electromyographic signals from some of the prominent steering muscles in order to correlate kinematics with muscle activity. Using this analysis, we found that the timing of each spike in the basalare muscles was strongly correlated with changes in the deviation of the stroke plane during the downstroke. The relationship was non-linear such that the magnitude of the kinematic response to each muscle spike decreased with increasing levels of stroke deviation. This result suggests that downstroke deviation is controlled in part via the mechanical summation of basalare activity. We also found that interactions among the basalares and muscles III2–III4 determine the maximum forward amplitude of the wingstroke. In addition, activity in muscle I1 appears to participate in a wingbeat gearing mechanism, as previously proposed. Using these results, we have been able to correlate changes in wing kinematics with alteration in the spike rate, firing phase and combinatorial activity of identified steering muscles.
Longitudinal variation in muscle protein expression and contraction kinetics of largemouth bass axial muscle
Authors: Thys Tierney M. , Blank Jason M. , Coughlin David J. , Schachat Fred
Published: April 2021

The present study investigates muscle protein expression in largemouth bass Micropterus salmoides through intra- and intermyomeric comparisons of white muscle. Using denaturing SDS-polyacrylamide gel electrophoresis, muscle protein expression in the arm and cone regions of sequential myomeres was compared for three bass. Low percentage (4.75 %) polyacrylamide-SDS gels and cyanogen bromide (CNBr) peptide mapping revealed no obvious intramyomeric differences between the myosin heavy chains of the arm and cone regions. Electrophoresis of myofibrils and muscle homogenates on higher percentage gels also failed to demonstrate any significant differences between arm and cone regions in either the myosin light chains or any of the major insoluble and soluble contractile proteins. Two differences were discovered intermyomerically: (i) the ratio of two troponin T isoforms changed from head to tail and (ii) caudal muscle had a lower total parvalbumin content than rostral muscle. Since troponin T and parvalbumin have been implicated in the regulation of skeletal muscle kinetics, longitudinal variation in muscle contraction kinetics was predicted. Subsequent experiments revealed that bass rostral white muscle showed faster rates of activation and relaxation than more caudal muscle, as has been observed in white muscle of other fish species. Rostral–caudal variations in white muscle protein composition and contractile properties are predicted to affect patterns of power production during fast, unsteady swimming.
Selective ablation of antennular sensilla on the Caribbean spiny lobsterPanulirus argussuggests that dual antennular chemosensory pathways mediate odorant activation of searching and localization of food
Authors: Steullet Pascal , Dudar Omar , Flavus Tanya , Zhou Min , Derby Charles D.
Published: April 2021

In spiny lobsters and other decapod crustaceans, odorant-mediated searching behavior patterns are driven primarily by chemosensory neurons in the antennules. Two groups of antennular chemosensory neurons can be distinguished on the basis of the sensilla that they innervate and their central projections: those that innervate the aesthetasc sensilla on the lateral flagella and project into the glomerularly organized olfactory lobes, and those that innervate other (i.e. non-aesthetasc) sensilla on both lateral and medial flagella and project into the stratified and non-glomerularly organized lateral antennular neuropils. By ablating different groups of antennular sensory neurons or sensilla, we examined the role of aesthetasc and non-aesthetasc chemosensory neurons in regulating local searching behavior of Caribbean spiny lobsters, Panulirus argus, for food (squid) in a low-flow environment. The results show that odorant-mediated activation of searching and localization of food under these conditions requires only a subset of functional antennular chemosensory neurons, since neither aesthetasc chemosensory neurons nor non-aesthetasc chemosensory neurons are by themselves necessary for these types of behavior. However, ablation of aesthetasc chemosensory neurons together with subsets of non-aesthetasc chemosensory neurons from either the medial or lateral flagella impairs the ability of lobsters to locate the food. This reveals a large degree of functional redundancy but also some complementary functions between aesthetasc and non-aesthetasc chemosensory neurons, and hence between these dual antennular chemosensory pathways, in odorant-mediated searching behavior of lobsters under these conditions.
Selection for high voluntary wheel-running increases speed and intermittency in house mice (Mus domesticus)
Authors: Girard I. , McAleer M. W. , Rhodes J. S. , Garland T.
Published: April 2021

In nature, many animals use intermittent rather than continuous locomotion. In laboratory studies, intermittent exercise regimens have been shown to increase endurance compared with continuous exercise. We hypothesized that increased intermittency has evolved in lines of house mice (Mus domesticus) that have been selectively bred for high voluntary wheel-running (wheel diameter 1.12 m) activity. After 23 generations, female mice from four replicate selection lines ran 2.7 times more revolutions per day than individuals from four random-bred control lines. To measure instantaneous running speeds and to quantify intermittency, we videotaped mice (N=41) during a 5-min period of peak activity on night 6 of a 6-day exposure to wheels. Compared with controls (20 revs min–1 while actually running), selection-line females (41 revs min–1) ran significantly faster. These instantaneous speeds closely matched the computer-recorded speeds over the same 5-min period. Selection-line females also ran more intermittently, with shorter (10.0 s bout–1) and more frequent (7.8 bouts min–1) bouts than controls (16.8 s bout–1, 3.4 bouts min–1). Inter-bout pauses were also significantly shorter in selection-line (2.7 s) than in control-line (7.4 s) females. We hypothesize that intermittency of locomotion is a key feature allowing the increased wheel-running performance at high running speeds in selection-line mice.
Similitude in the Cardiovascular System of Mammals
Authors: Dawson Thomas H.
Published: April 2021

Scaling laws governing the cardiovascular system of mammals are discussed in the present review in a manner emphasizing their experimental basis. Specific attention is given to the well-known experimental laws requiring the rate of oxygen consumption and the heart rate of mammals to vary with body mass raised to the powers 3/4 and −1/4, respectively. This review involves reconsideration and further discussion of the previous work of the writer in which these and other scaling relationships were developed from fundamental considerations. The predicted scaling laws remain unchanged from the earlier work, but alternative assumptions leading to the laws are used so as to provide additional insight. The scaling laws are shown to have their origin in the basic design of the cardiovascular system and in the basic processes involved in its working. Modification of the design assumptions of the system to account for known differences in the relative heart masses of mammals and birds is shown to lead to the scaling laws for rate of oxygen consumption and heart rate of birds.
The Influence of Thermal Acclimation on Power Production During Swimming I. In Vivo Stimulation and Length Change Pattern of Scup Red Muscle
Authors: Rome Lawrence C. , Swank Douglas M.
Published: April 2021

Ectothermal animals are able to locomote in a kinematically similar manner over a wide range of temperatures. It has long been recognized that there can be a significant reduction in the power output of muscle during swimming at low temperatures because of the reduced steady-state (i.e. constant activation and shortening velocity) power-generating capabilities of muscle. However, an additional reduction in power involves the interplay between the non-steady-state contractile properties of the muscles (i.e. the rates of activation and relaxation) and the in vivo stimulation and length change pattern the muscle undergoes during locomotion. In particular, it has been found that isolated scup (Stenotomus chrysops) red muscle working under in vivo stimulus and length change conditions (measured in warm-acclimated scup swimming at low temperatures) generates very little power for swimming. Even though the relaxation of the muscle has slowed greatly, warm-acclimated fish swim with the same tail-beat frequencies and the same stimulus duty cycles at cold temperatures, thereby not affording the slow-relaxing muscle any extra time to relax.

We hypothesize that considerable improvement in the power output of the red muscle at low temperatures could be achieved if cold acclimation resulted in either a faster muscle relaxation rate or in the muscle being given more time to relax (e.g. by shortening the stimulus duration or reducing the tail-beat frequency). We test these hypotheses in this paper and the accompanying paper.

Scup were acclimated to 10 °C (cold-acclimated) and 20 °C (warm-acclimated) for at least 6 weeks. Electromyograms (EMGs) and high-speed cine films were taken of fish swimming steadily at 10 °C and 20 °C. At 10 °C, we found that, although there were no differences in tail-beat frequency, muscle strain or stimulation phase between acclimation groups, cold-acclimated scup had EMG duty cycles approximately 20 % shorter than warm-acclimated scup. In contrast at 20 °C, there was no difference between acclimation groups in EMG duty cycle, nor in any other muscle length change or stimulation parameter.

Thus, in response to cold acclimation, there appears to be a reduction in EMG duty cycle at low swimming temperatures that is probably due to an alteration in the operation of the pattern generator. This novel acclimation probably improves muscle power output at low temperatures compared with that of warm-acclimated fish, an expectation we test in the accompanying paper using the work-loop technique.
Wake Dynamics and Fluid Forces of Turning Maneuvers in Sunfish
Authors: Drucker Eliot G. , Lauder George V.
Published: April 2021

While experimental analyses of steady rectilinear locomotion in fishes are common, unsteady movement involving time-dependent variation in heading, speed and acceleration probably accounts for the greatest portion of the locomotor time budget. Turning maneuvers, in particular, are key elements of the unsteady locomotor repertoire of fishes and, by many species, are accomplished by generating asymmetrical forces with the pectoral fins. The development of such left–right asymmetries in force production is a critical and as yet unstudied aspect of aquatic locomotor dynamics. In this paper, we measure the fluid forces exerted by the left and right pectoral fins of bluegill sunfish (Lepomis macrochirus) during turning using digital particle image velocimetry (DPIV). DPIV allowed quantification of water velocity fields, and hence momentum, in the wake of the pectoral fins as sunfish executed turns; forces exerted during turning were compared with those generated by the immediately preceding fin beats during steady swimming. Sunfish generate the forces required for turning by modulating two variables: wake momentum and pectoral fin stroke timing. Fins on opposite sides of the fish play functionally distinct roles during turning maneuvers. The fin nearer the stimulus inducing the turn (i.e. the strong side fin) generates a laterally oriented vortex ring with a strong central jet whose associated lateral force is four times greater than that produced during steady swimming. Little posterior (thrust) force is generated by the strong-side fin, and this fin therefore acts to rotate the body away from the source of the stimulus. The contralateral (weak-side) fin generates a posteriorly oriented vortex ring with a thrust force nine times that produced by the fin during steady swimming. Minimal lateral force is exerted by the weak-side fin, and this fin therefore acts primarily to translate the body linearly away from the stimulus. Turning with the paired fins is not simply steady swimming performed unilaterally. Instead, turning involves asymmetrical fin movements and fluid forces that are distinct in both direction and magnitude from those used to swim forward at constant speed. These data reflect the plasticity of the teleost pectoral fin in performing a wide range of steady and unsteady locomotor tasks.
Kinematics of Rotation in Place During Defense Turning in the Crayfish Procambarus Clarkii
Authors: Copp Newton H. , Jamon Marc
Published: April 2021

The kinematic patterns of defense turning behavior in freely behaving specimens of the crayfish Procambarus clarkii were investigated with the aid of a video-analysis system. Movements of the body and all pereiopods, except the chelipeds, were analyzed. Because this behavior approximates to a rotation in place, this analysis extends previous studies on straight and curve walking in crustaceans.

Specimens of P. clarkii responded to a tactile stimulus on a walking leg by turning accurately to face the source of the stimulation. Angular velocity profiles of the movement of the animal’s carapace suggest that defense turn responses are executed in two phases: an initial stereotyped phase, in which the body twists on its legs and undergoes a rapid angular acceleration, followed by a more erratic phase of generally decreasing angular velocity that leads to the final orientation. Comparisons of contralateral members of each pair of legs reveal that defense turns are affected by changes in step geometry, rather than by changes in the timing parameters of leg motion, although inner legs 3 and 4 tend to take more steps than their outer counterparts during the course of a response. During the initial phase, outer legs 3 and 4 exhibit larger stance amplitudes than their inner partners, and all the outer legs produce larger stance amplitudes than their inner counterparts during the second stage of the response. Also, the net vectors of the initial stances, particularly, are angled with respect to the body, with the power strokes of the inner legs produced during promotion and those of the outer legs produced during remotion. Unlike straight and curve walking in the crayfish, there is no discernible pattern of contralateral leg coordination during defense turns. Similarities and differences between defense turns and curve walking are discussed. It is apparent that rotation in place, as in defense turns, is not a simple variation on straight or curve walking but a distinct locomotor pattern.
Pectoralis Muscle Performance During Ascending and Slow Level Flight in Mallards (Anas Platyrhynchos)
Authors: Williamson Michael R. , Dial Kenneth P. , Biewener Andrew A.
Published: April 2021

In vivo measurements of pectoralis muscle length change and force production were obtained using sonomicrometry and delto-pectoral bone strain recordings during ascending and slow level flight in mallards (Anas platyrhynchos). These measurements provide a description of the force/ length properties of the pectoralis under dynamic conditions during two discrete flight behaviors and allow an examination of the effects of differences in body size and morphology on pectoralis performance by comparing the results with those of a recent similar study of slow level flight in pigeons (Columbia livia). In the present study, the mallard pectoralis showed a distinct pattern of active lengthening during the upstroke. This probably enhances the rate of force generation and the magnitude of the force generated and, thus, the amount of work and power produced during the downstroke. The power output of the pectoralis averaged 17.0 W kg−1 body mass (131 W kg−1muscle mass) during slow level flight (3 m s−1) and 23.3 W kg−1 body mass (174 W kg−1 muscle mass) during ascending flight. This increase in power was achieved principally via an increase in muscle strain (29 % versus 36 %), rather than an increase in peak force (107 N versus 113 N) or cycle frequency (8.4 Hz versus 8.9 Hz). Body-mass-specific power output of mallards during slow level flight (17.0 W kg−1), measured in terms of pectoralis mechanical power, was similar to that measured recently in pigeons (16.1 W kg−1). Mallards compensate for their greater body mass and proportionately smaller wing area and pectoralis muscle volume by operating with a high myofibrillar stress to elevate mechanical power output.
Prey-Capture Behavior in Gymnotid Electric Fish: Motion Analysis and Effects of water Conductivity
Authors: Maciver Malcolm A. , Sharabash Noura M. , Nelson Mark E.
Published: April 2021

Animals can actively influence the content and quality of sensory information they acquire from the environment through the positioning of peripheral sensory surfaces. This study investigated receptor surface positioning during prey-capture behavior in weakly electric gymnotiform fish of the genus Apteronotus. Infrared video techniques and three-dimensional model-based tracking methods were used to provide quantitative information on body position and conformation as black ghost (A. albifrons) and brown ghost (A. leptorhynchus) knifefish hunted for prey (Daphnia magna) in the dark. We found that detection distance depends on the electrical conductivity of the surrounding water. Best performance was observed at low water conductivity (2.8 cm mean detection distance and 2 % miss rate at 35 μS cm−1, A. albifrons) and poorest performance at high conductivity (1.5 cm mean detection distance and 11 % miss rate at 600 μS cm−1, A. albifrons). The observed conductivity-dependence implies that nonvisual prey detection in Apteronotus is likely to be dominated by the electrosense over the range of water conductivities experienced by the animal in its natural environment. This result provides the first evidence for the involvement of electrosensory cues in the prey-capture behavior of gymnotids, but it leaves open the possibility that both the high-frequency (tuberous) and low-frequency (ampullary) electroreceptors may contribute. We describe an electrosensory orienting response to prey, whereby the fish rolls its body following detection to bring the prey above the dorsum. This orienting response and the spatial distribution of prey at the time of detection highlight the importance of the dorsal surface of the trunk for electrosensory signal acquisition. Finally, quantitative analysis of fish motion demonstrates that Apteronotus can adapt its trajectory to account for post-detection motion of the prey, suggesting that it uses a closed-loop adaptive tracking strategy, rather than an open-loop ballistic strike strategy, to intercept the prey.
Behavioural Assessment of Visual Acuity in Bumblebees (Bombus Impatiens)
Authors: Macuda Todd , Gegear Robert J. , Laverty Terence M. , Timney Brian
Published: April 2021

The present study used previously developed techniques to measure resolution acuity in bumblebees (Bombus impatiens). Bees were required to discriminate between horizontal and vertical gratings in a Y-maze apparatus. The gratings had a mean luminance of 9 cd m−2 and a Michelson contrast of 84 %. For different bees, either the horizontal or vertical grating was rewarded. Rewarded gratings were associated with a sucrose and water solution (30 % sucrose by volume) and unrewarded gratings with plain water. Acuity estimates were established at several different viewing distances over several sessions using a method of constant stimuli. Visual acuity functions were generated from the performance data, and acuity thresholds were interpolated at a performance level of 65 % correct. When corrected for viewing distance, best angular acuity obtained for horizontal and vertical gratings was 0.35 and 0.36 cycles degree−1, respectively. These results are compared with those of the honeybee and discussed in the context of the bumblebee’s foraging behaviour.
Quantitative Tests of an Associative Theory of Risk-Sensitivity in Honeybees
Authors: Shapiro Martin S. , Couvillon P. A. , Bitterman M. E.
Published: April 2021

Risk-sensitivity was studied in free-flying honeybees trained individually to choose between two scented targets (A and B) with varying amounts and concentrations of sucrose solution as reward. In the first phase of experiment 1, the animals showed ‘risk-aversion,’ preferring A, which provided 5 μl of a 40 % sucrose solution on every trial, to B, which provided 30 μl of the same solution once in every six trials (mean amount per trial 5 μl for each alternative). In the second phase, the preference reversed with reversal of the reward assignments. In experiment 2, the consistently rewarded A (5 μl of 40 % sucrose solution per trial) was again preferred, although the inconsistently rewarded B now provided twice the amount of sucrose solution on average (30 μl on two of every six trials, mean amount per trial 10 μl). In experiment 3, with A providing 10 μl of a 15 % sucrose solution on every trial and B providing 10 μl of a 60 % sucrose solution on two of every four trials (mean concentration per trial 30 %), the animals preferred B. In Experiment 4, patterned after experiment 1, similar results were obtained under more natural conditions in which the animals were no longer constrained (as they were in the first three experiments) to go equally often to each alternative. The results of all four experiments were predicted quantitatively and with considerable accuracy by a simple associative theory of discriminative learning in honeybees.
The Synchronisation of Signal Emission with Wingbeat During the Approach Phase in Soprano Pipistrelles (Pipistrellus Pygmaeus)
Authors: Wong Josephine G. , Waters Dean A.
Published: April 2021

Previous studies have shown that, during search flight in bats, wingbeat, respiration and echolocation are synchronised in a 1:1 relationship. An efficiently integrated locomotor–respiratory system enables bats to produce intense echolocation signals at little or no cost above that required for flight. In this study, we investigated the coupling of wingbeat with echolocation in the laboratory during approach flight in soprano pipistrelles (Pipistrellus pygmaeus) using high-speed digital video at 250 and 500 frames s−1. We found that soprano pipistrelles most commonly produce single or double pulses per wingbeat. Single pulses per wingbeat occurred in two alternative positions: immediately before the end of the upstroke or after the start of the downstroke. Double pulses per wingbeat were emitted in the same wingbeat positions on the upstroke and the downstroke, as in single pulses per wingbeat. We suggest that, during approach flight, the coupling of more than one echolocation signal with a single wingbeat and expiratory cycle allows echolocation to remain energetically economic. When soprano pipistrelles approached a Perspex disc target, an increase in mean repetition rate was achieved by producing an extra pulse per wingbeat. Finally, we hypothesise that the bat’s approach to potentially interesting targets in the same horizontal plane as it’s flight path, i.e. during flapping flight, may be characterised by the production of double pulses per wingbeat, resulting in a unique pattern of echolocation pulse intervals.
Olfactory and Behavioural Responses of the Blood-Sucking bug Triatoma Infestans to Odours of Vertebrate Hosts
Authors: Guerenstein P. G. , Guerin P. M.
Published: April 2021

Olfactory receptors in basiconic and grooved-peg sensilla on the antenna of fifth-instar Triatoma infestans nymphs respond to host odours. Gas chromatography analyses of host odour extracts coupled to electrophysiological recordings from basiconic sensillum receptors indicate that nonanal is a constituent of sheep wool and chicken feather odour that stimulates one of the receptors in this type of sensillum. Similar analyses revealed isobutyric acid in rabbit odour to be a chemostimulant for one of the receptors in grooved-peg sensilla. The response of the aldehyde receptor was higher to heptanal, octanal and nonanal than to other aliphatic aldehydes, and the response of the acid receptor was higher to isobutyric acid than to other short-chain branched and unbranched acids.

The behavioural responses of fifth-instar T. infestans nymphs to nonanal and isobutyric acid in an air-stream on a servosphere indicate that, whereas nonanal causes activation of the bugs, isobutyric acid induces an increase in upwind displacement, i.e. odour-conditioned anemotaxis. Binary mixtures of these compounds did not improve the attraction obtained with isobutyric acid alone. A comparison of the behavioural and electrophysiological responses of the bugs to different amounts of isobutyric acid in air suggests that attraction is obtained at concentrations that causes low-to-moderate increases in the firing rate of the acid-excited receptor in the grooved-peg sensilla, whereas at a dose that evokes relatively high firing rates (>40 Hz) no attraction is obtained.
Coordinated Righting Behaviour in Locusts
Authors: Faisal A. A. , Matheson T.
Published: April 2021

A locust placed upside down on a flat surface uses a predictable sequence of leg movements to right itself. To analyse this behaviour, we made use of a naturally occurring state of quiescence (thanatosis) to position locusts in a standardised upside-down position from which they spontaneously right themselves.

Locusts grasped around the pronotum enter a state of thanatosis during which the limbs can be manipulated into particular postures, where they remain, and the animal can be placed upside down on the ground. When released, thanatosis lasts 4–456 s (mean 73 s) before the animal suddenly becomes active again and rights itself within a further 600 ms. Thanatosis is characterised by very low levels of leg motor activity.

During righting, one hind leg provides most of the downward force against the ground that rolls the body around a longitudinal axis towards the other side. The driving force is produced by femoral levation (relative to the body) at the trochanter and by tibial extension. As the animal rolls over, the hind leg on the other side is also levated at the trochanter, so that it does not obstruct the movement. The forelegs and middle legs are not required for successful righting but they can help initially to tip the locust to one side, and at the end of the movement they help stop the roll as the animal turns upright. Individual locusts have a preferred righting direction but can, nevertheless, roll to either side.

Locusts falling upside down through the air use both passive and active mechanisms to right themselves before they land. Without active movements, falling locusts tend to rotate into an upright position, but most locusts extend their hind leg tibiae and/or spread their wings, which increases the success of mid-air righting from 28 to 49 % when falling from 30 cm.

The rapid and reliable righting behaviour of locusts reduces the time spent in a vulnerable upside-down position. Their narrow body geometry, large hind legs, which can generate substantial dorsally directed force, and the particular patterns of coordinated movements of the legs on both sides of the body are the key features that permit locusts to right themselves effectively. The reliability of autonomous multi-legged robots may be enhanced by incorporating these features into their design.
Heart Rate and Rate of Oxygen Consumption of Exercising Macaroni Penguins
Authors: Green J. A. , Butler P. J. , Woakes A. J. , Boyd I. L. , Holder R. L.
Published: April 2021

Summary Twenty-four macaroni penguins (Eudyptes chrysolophus) from three groups, breeding males (N=9), breeding females (N=9) and moulting females (N=6), were exercised on a variable-speed treadmill. Heart rate (fH) and mass-specific rate of oxygen consumption were recorded from the animals, and both fH and were found to increase linearly with increasing treadmill speed. A linear regress.ion equation described the relationship between Fh and for each individual. There were no significant differences in these regressions between breeding and moulting females. There were significant differences in these relationships between all females and breeding males. fH and were recorded from five of these animals for a total of 24 h. When fH was used to predict for the 24 h period using the derived regressions, the estimate was not significantly different from the measured values, with .an average error of −2.1 %. When fH was used to predict for the 5 min intervals used for the calibration in all 24 birds, the estimate was not significantly different from the observed value. s, and the average error was only +0.47 %. Since the relationship was the same during periods of the annual cycle when the animals were inactive/fasting and active/foraging, it seems reasonable that, as long as sex differences are taken into account, fH can be used to predict the metabolic rates of free-ranging macaroni penguins all year round.
A New Technique for Monitoring the Behaviour of Free-Ranging Adélie Penguins
Authors: Yoda K. , Naito Y. , Sato K. , Takahashi A. , Nishikawa J. , Ropert-Coudert Y. , Kurita M. , Maho Y. Le
Published: April 2021

Measurement of the time allocation of penguins at sea has been a major goal of researchers in recent years. Until now, however, no equipment has been available that would allow measurement of the aquatic and terrestrial behaviour of an Antarctic penguin while it is commuting between the colony and the foraging grounds. A new motion detector, based on the measurement of acceleration, has been used here in addition to current methods of inferring behaviour using data loggers that monitor depth and speed. We present data on the time allocation of Adélie penguins (Pygoscelis adeliae) according to the different types of behaviours they display during their foraging trips: walking, tobogganing, standing on land, lying on land, resting at the water surface, porpoising and diving. To illustrate the potential of this new technique, we compared the behaviour of Adélie penguins during the chick-rearing period in a fast sea-ice region and an ice-free region. The proportion of time spent standing, lying on land and walking during foraging trips was greater for penguins in the sea-ice region (37.6±13.3 % standing, 21.6±15.6 % lying and 5.9±6.3 % walking) than for those in the ice-free region (12.0±15.8 % standing, 0.38±0.60 % lying and 0 % walking), whereas the proportion of time spent resting at the water surface and porpoising was greater for birds in the ice-free region (38.1±6.4 % resting and 1.1±1.1 % porpoising) than for those in the sea-ice region (3.0±2.3 % resting and 0 % porpoising; means ± S.D., N=7 for the sea-ice region, N=4 for the ice-free region). Using this new approach, further studies combining the monitoring of marine resources in different Antarctic sites and the measurement of the energy expenditure of foraging penguins, e.g. using heart rates, will constitute a powerful tool for investigating the effects of environmental conditions on their foraging strategy. This technique will expand our ability to monitor many animals in the field.