bluuebunny/crossref_metadata_2025 huggingface dataset:
Strength Analysis of a Rib-Stiffened GLARE-Based Thin-Walled Structure
Authors: Kubit Andrzej , Trzepieciński Tomasz , Krasowski Bogdan , Slota Ján , Spišák Emil
Published: June 2020

This paper presents a new product, a glass laminate aluminium-reinforced epoxy (GLARE)-based thin-walled structure with a stiffener in the form of a longitudinal rib. The stiffening rib in an outer metallic layer of a GLARE-based panel was fabricated by the incremental sheet forming technique and Alclad 2024-T3 aluminium alloy sheets were used as adherends. The strength properties of the adhesive joint between the layers of the fibre metal laminates (FMLs) were determined in a uniaxial tensile test, peel drum test, tensile/shear test and short-beam three-point-bending test. Two variants of FMLs were considered, with an adhesive film and without an adhesive film between the adherends and the epoxy/glass prepreg. The FMLs were tested at three different temperatures that corresponded to those found under real aircraft operating conditions, i.e., −60 °C, room temperature and +80 °C. It was found that the temperatures do not affect the tensile strength and shear strength of the FMLs tested. However, there was a noticeable increase in the stiffness of samples stretched at reduced temperature. An additional adhesive film layer between the adherends and the glass/epoxy prepreg significantly improves the static peeling strength of the joint both at reduced and at elevated temperatures. A clear increase in the critical force at which buckling occurs has been clearly demonstrated in the uniaxial compression test of GLARE-based rib-stiffened panels. In the case of GLARE-based rib-stiffened panels, the critical force averaged 15,370 N, while for the non-embossed variant, it was 11,430 N, which translates into a 34.5% increase in critical force.
Heating Conditions Influence on Solidification of Inconel 625–WC System for Additive Manufacturing
Authors: Huebner Jan , Rutkowski Paweł , Dębowska Aleksandra , Kata Dariusz
Published: June 2020

In this study, an Inconel 625–tungsten carbide (WC) composite system was investigated by means of microstructure changes affected by both heating rate and WC content. In order to investigate how the system behaves while exposed to fast thermal processing, controlled melting using a differential thermal analysis (DTA) apparatus was performed on the powders. Two WC powders with different average grain size were used to obtain six compositions of Inconel 625–WC powder mixtures (10, 20, and 30 wt.% WC). They were analyzed under 10 and 30 °C/min heating rate in order to obtain composite samples. Results from DTA together with SEM/energy-dispersive X-ray spectroscopy (EDS) microstructural observations allowed observing material changes during solidification. Because of the extensive microsegregation of alloying elements to liquid and their reactions with C, which derived from dissolved WC, the formation of secondary phases with improved microhardness was possible. The collected results provide a better understanding of material behavior during intensive thermal processing which can be useful when designing materials for the laser additive manufacturing technique.
New Oral Surgery Materials for Bone Reconstruction—A Comparison of Five Bone Substitute Materials for Dentoalveolar Augmentation
Authors: Kozakiewicz Marcin , Wach Tomasz
Published: June 2020

This article presents a comparison of bone replacement materials in terms of their ability to produce living bone image at the place of their implantation. Five bone replacement materials are compared (Osteovit—porous collagen, Cerasorb Foam—collagen scaffolding of synthetic β tricalcium phosphate, Osbone—synthetic hydroxyapatite, Endobone—deproteinized bovine-derived cancellous bone hydroxyapatite, and Cerasorb—synthetic β tricalcium phosphate). Intraoral radiographs are taken immediately after implantation and 12 months later. The texture analysis was performed to assess (texture index, TI) the level of structure chaos (entropy) in relation to the presence of longitudinal elements visible in radiographs (run length emphasis moment). The reference ratio of the chaotic trabecular pattern (Entropy) to the number of longitudinal structures, i.e., trabeculae (LngREmph), is 176:100 (i.e., 1.76 ± 0.28). Radiological homogeneity immediately after the implantation procedure is a result of the similar shape of its particles (Osbone, Endobone and Cerasorb) or radiolucency (Osteovit, Cerasorb Foam). The particles visible in radiographs were similar in the LngREmph parameters applied to the reference bone, but not in the co-occurrence matrix features. The TI for Osteovit during a 12-month follow-up period changed from 1.55 ± 0.26 to 1.48 ± 0.26 (p > 0.05), for Cerasorb Foam from 1.82 ± 0.27 to 1.63 ± 0.24 (p < 0.05), for Osbone from 1.97 ± 0.31 to 1.74 ± 0.30 (p < 0.01), and for Endobone from 1.86 ± 0.25 to 1.84 ± 0.25 (p > 0.05), The observed structure in the radiological image of bone substitute materials containing calcium phosphates obtains the characteristics of a living bone image after twelve months.
Orthogonal Analysis on Mechanical Properties of Basalt–Polypropylene Fiber Mortar
Authors: Chen Huimin , Xie Chunyan , Fu Chao , Liu Jing , Wei Xiuli , Wu Dake
Published: June 2020

Orthogonal test method was applied to analyze the strength properties of basalt-polypropylene mortar. The effect of basalt fiber length, polypropylene fiber length, basalt fiber volume content and polypropylene fiber volume content on the 28 d cube compressive strength and flexural strength were investigated. Test results show that comparing with flexural strength, the influence of basalt fiber length and polypropylene fiber length on compressive strength of mortar was greater than on flexural strength. The length of polypropylene fibers contributes the highest to the flexural strength. The effect of basalt fiber on mortar strength is the largest with 6 mm length and 4% content. Polypropylene fiber length has the greatest influence on the compressive strength of fiber mortar, followed by basalt fiber volume content. Volume content of polypropylene fiber has the greatest influence on flexural strength of fiber mortar, followed by polypropylene fiber length. According to the scoring of the efficacy coefficient method, the best ratio combination for compressive and flexural strength was the basalt fiber length of 9 mm, polypropylene fiber length of 6 mm, basalt fiber volume content of 4% and polypropylene fiber volume content of 4%. Compared with the blank samples, the 28 d compressive strength and 28 d flexural strength of the cement mortar samples were increased by 27.4% and 49% respectively. According to the test results, the properties of the fiber were analyzed and evaluated and the mechanism of fiber action and fiber microstructure were analyzed.
Wet Synthesis of Elongated Hexagonal ZnO Microstructures for Applications as Photo-Piezoelectric Catalysts
Authors: Pagano Rosanna , Ingrosso Chiara , Giancane Gabriele , Valli Ludovico , Bettini Simona
Published: June 2020

It is well known that energetic demand and environmental pollution are strictly connected; the side products of vehicle and industrial exhausts are considered extremely dangerous for both human and environmental health. In the last years, the possibility to simultaneously photo-degrade water dissolved pollutants by means of ZnO nanostructures and to use their piezoelectric features to enhance the photo-degradation process has been investigated. In the present contribution, an easy and low-cost wet approach to synthetize hexagonal elongated ZnO microstructures in the wurtzite phase was developed. ZnO performances as photo-catalysts, under UV-light irradiation, were confirmed on water dissolved methylene blue dye. Piezoelectric responses of the synthetized ZnO microstructures were evaluated, as well, by depositing them into films onto flexible substrates, and a home-made layout was developed, in order to stimulate the ZnO microstructures deposited on solid supports by means of mechanical stress and UV photons, simultaneously. A relevant increment of the photo-degradation efficiency was observed when the piezopotential was applied, proposing the present approach as a completely eco-friendly tool, able to use renewable energy sources to degrade water solved pollutants.
On the Seismic Performance of Autoclaved Aerated Concrete Self-Insulation Block Walls
Authors: Liu Yun , Chen Gonglian , Wang Zhipeng , Chen Zhen , Gao Yujia , Li Fenglan
Published: June 2020

Autoclaved aerated concrete (AAC) self-insulation block masonry is often used for the infill walls in steel and concrete frame structures. To work together with the frame under earthquake action, it is essential to understand the seismic behavior of AAC self-insulation block masonry walls. In this paper, six AAC self-insulation block masonry walls were experimentally studied under the pseudo static test. The load-displacement hysteretic curves were drawn with the test data. The failure characteristics, loading capacity, stiffness degeneration, energy dissipation capacity and hysteretic behavior are analyzed. The results indicate that the blocks underwent internal failure due to the lower strength with a larger size, but the walls had good energy dissipation capacity with a rational bearing capacity. Accompanied by the influence of vertical compressive stress on the top surface of the walls, the cracking resistance, ultimate bearing capacity, deformability and energy dissipation capacity of the walls were affected by the masonry mortar joints. Comparatively, the walls with thin-layer mortar joints had better seismic performance than those with insulation mortar joints or with vertical joints filled by mineral wool plates. Finally, the shear capacity of the walls under seismic load is evaluated referring to the formulas of current design codes for masonry walls.
On the Nonlinear Stability and Instability of the Boussinesq System for Magnetohydrodynamics Convection
Authors: Bian Dongfen
Published: June 2020

This paper is concerned with the nonlinear stability and instability of the two-dimensional (2D) Boussinesq-MHD equations around the equilibrium state ( u ¯ = 0 , B ¯ = 0 , θ ¯ = θ 0 ( y ) ) with the temperature-dependent fluid viscosity, thermal diffusivity and electrical conductivity in a channel. We prove that if a + ≥ a − , and d 2 d y 2 κ ( θ 0 ( y ) ) ≤ 0 or 0 < d 2 d y 2 κ ( θ 0 ( y ) ) ≤ β 0 , with β 0 > 0 small enough constant, and then this equilibrium state is nonlinearly asymptotically stable, and if a + < a − , this equilibrium state is nonlinearly unstable. Here, a + and a − are the values of the equilibrium temperature θ 0 ( y ) on the upper and lower boundary.
A Multi-Criteria Decision-Making Method Based on the Improved Single-Valued Neutrosophic Weighted Geometric Operator
Authors: Tian Chao , Peng Juan Juan
Published: June 2020

The aggregation operator is one of the most common techniques to solve multi-criteria decision-making (MCDM) problems. The aim of this paper is to propose an MCDM method based on the improved single-valued neutrosophic weighted geometric (ISVNWG) operator. First, the defects of several existing single-valued neutrosophic weighted geometric aggregation operators in terms of producing uncertain results in some special cases are analyzed. Second, an ISVNWG operator is proposed to avoid the defects of existing operators. Further, the properties of the proposed ISVNWG operator, including idempotency, boundedness, monotonicity, and commutativity, are discussed. Finally, a single-valued neutrosophic MCDM method based on the developed ISVNWG operator is proposed to overcome the defects of existing MCDM methods based on existing operators. Application examples demonstrate that our proposed operator and corresponding MCDM method are effective and rational for avoiding uncertain results in some special cases.
Unexpected Enhancement of HDACs Inhibition by MeS Substitution at C-2 Position of Fluoro Largazole
Authors: Zhang Bingbing , Ruan Zhu-Wei , Luo Dongdong , Zhu Yueyue , Ding Tingbo , Sui Qiang , Lei Xinsheng
Published: June 2020

Given our previous finding that fluorination at the C18 position of largazole showed reasonably good tolerance towards inhibitory activity and selectivity of histone deacetylases (HDACs), further modification on the valine residue in the fluoro-largazole’s macrocyclic moiety with S-Me l-Cysteine or Glycine residue was performed. While the Glycine-modified fluoro analog showed poor activity, the S-Me l-Cysteine-modified analog emerged to be a very potent HDAC inhibitor. Unlike all previously reported C2-modified compounds in the largazole family (including our recent fluoro-largazole analogs) where replacement of the Val residue has failed to provide any potency improvement, the S-Me l-Cysteine-modified analog displayed significantly enhanced (five–nine-fold) inhibition of all the tested HDACs while maintaining the selectivity of HDAC1 over HDAC6, as compared to largazole thiol. A molecular modeling study provided rational explanation and structural evidence for the enhanced inhibitory activity. This new finding will aid the design of novel potent HDAC inhibitors.
Marine-Derived Polymers in Ionic Liquids: Architectures Development and Biomedical Applications
Authors: Silva Simone S. , Gomes Joana M. , Rodrigues Luísa C. , Reis Rui L.
Published: June 2020

Marine resources have considerable potential to develop high-value materials for applications in different fields, namely pharmaceutical, environmental, and biomedical. Despite that, the lack of solubility of marine-derived polymers in water and common organic solvents could restrict their applications. In the last years, ionic liquids (ILs) have emerged as platforms able to overcome those drawbacks, opening many routes to enlarge the use of marine-derived polymers as biomaterials, among other applications. From this perspective, ILs can be used as an efficient extraction media for polysaccharides from marine microalgae and wastes (e.g., crab shells, squid, and skeletons) or as solvents to process them in different shapes, such as films, hydrogels, nano/microparticles, and scaffolds. The resulting architectures can be applied in wound repair, bone regeneration, or gene and drug delivery systems. This review is focused on the recent research on the applications of ILs as processing platforms of biomaterials derived from marine polymers.
Anion Exchange Membranes Prepared from Quaternized Polyepichlorohydrin Cross-Linked with 1-(3-aminopropyl)imidazole Grafted Poly(arylene ether ketone) for Enhancement of Toughness and Conductivity
Authors: Tuan Cao Manh , Cong Tinh Vo Dinh , Kim Dukjoon
Published: June 2020

A novel anion exchange membrane was synthesized via crosslinking of the quaternized polyepichlorohydrin (QPECH) by 1-(3-aminopropyl) imidazole grafted poly(arylene ether ketone) (PAEK-API). While the QPECH provided an excellent ion conductive property, the rigid rod-structured PAEK-API played a reinforcing role, along with providing the high conductivity associated with the pendant API group. The chemical structure of QPECH/PAEK-API membranes was identified by 1H nuclear magnetic resonace spectroscopy. A variety of membrane properties, such as anion conductivity, water uptake, length swelling percentage, and thermal, mechanical and chemical stability, were investigated. The QPECH/PAEK-API1 membrane showed quite high hydroxide ion conductivity, from 0.022 S cm−1 (30 °C) to 0.033 S cm−1 (80 °C), and excellent mechanical strength, associated with the low water uptake of less than 40%, even at 80 °C. Such high conductivity at relatively low water uptake is attributed to the concentrated cationic groups, in a cross-linked structure, facilitating feasible ion transport. Further, the QPECH/PAEK-API membranes showed thermal stability up to 250 °C, and chemical stability for 30 days in a 4 NaOH solution, without significant loss of ion exchange capacity.
Metabolic Profiling Provides Unique Insights to Accumulation and Biosynthesis of Key Secondary Metabolites in Annual Pasture Legumes of Mediterranean Origin
Authors: Latif Sajid , Weston Paul A. , Barrow Russell A. , Gurusinghe Saliya , Piltz John W. , Weston Leslie A.
Published: June 2020

Annual legumes from the Mediterranean region are receiving attention in Australia as alternatives to traditional pasture species. The current study employed novel metabolic profiling approaches to quantify key secondary metabolites including phytoestrogens to better understand their biosynthetic regulation in a range of field-grown annual pasture legumes. In addition, total polyphenol and proanthocyanidins were quantified using Folin–Ciocalteu and vanillin assays, respectively. Metabolic profiling coupled with biochemical assay results demonstrated marked differences in the abundance of coumestans, flavonoids, polyphenols, and proanthocyanidins in annual pasture legume species. Genetically related pasture legumes segregated similarly from a chemotaxonomic perspective. A strong and positive association was observed between the concentration of phytoestrogens and upregulation of the flavonoid biosynthetic pathway in annual pasture legumes. Our findings suggest that evolutionary differences in metabolic dynamics and biosynthetic regulation of secondary metabolites have logically occurred over time in various species of annual pasture legumes resulting in enhanced plant defense.
Micro Water Flow Measurement Using a Temperature-Compensated MEMS Piezoresistive Cantilever
Authors: Pommois Romain , Furusawa Gaku , Kosuge Takuya , Yasunaga Shun , Hanawa Haruki , Takahashi Hidetoshi , Kan Tetsuo , Aoyama Hisayuki
Published: June 2020

In this study, we propose a microelectromechanical system (MEMS) force sensor for microflow measurements. The sensor is equipped with a flow sensing piezoresistive cantilever and a dummy piezoresistive cantilever, which acts as a temperature reference. Since the dummy cantilever is also in the form of a thin cantilever, the temperature environment of the dummy sensor is almost identical to that of the sensing cantilever. The temperature compensation effect was measured, and the piezoresistive cantilever was combined with a gasket jig to enable the direct implementation of the piezoresistive cantilever in a flow tube. The sensor device stably measured flow rates from 20 μL/s to 400 μL/s in a silicon tube with a 2-mm inner diameter without being disturbed by temperature fluctuations.
Optimization of MOEMS Projection Module Performance with Enhanced Piezoresistive Sensitivity
Authors: Yu Huijun , Zhou Peng , Wang Kewei , Huang Yanfei , Shen Wenjiang
Published: June 2020

In scanning laser projection systems, the laser modulation time is important for the projection resolution. The modulation time needs to be matched with the motion of the micromirror. For this paper, the piezoresistive sensor was integrated on the torsion beam of the micromirror to monitor the physical position of the micromirror. The feedback signal was used to generate the zero-crossing time, which was used to estimate the physical position of the resonating mirror over time. The estimated position was affected by the zero-crossing time and the error directly influenced the definition of the projected image. By reducing the impurity concentration from 3 × 1018/cm3 to 1 × 1018/cm3 and increasing shear stress on piezoresistive sensor, the sensitivity of the piezoresistive sensor increased from 4.4 mV/V° to 6.4 mV/V° and the error of the image pixel reduced from 1.5 pixels to 0.5 pixels. We demonstrated that the image quality of an Optical-Microeletromechanical Systems (MOEMS) laser projection could be improved by enhancing the sensitivity of the piezoresistive sensor.
Mineralization Age and Hydrothermal Evolution of the Fukeshan Cu (Mo) Deposit in the Northern Great Xing’an Range, Northeast China: Evidence from Fluid Inclusions, H–O–S–Pb Isotopes, and Re–Os Geochronology
Authors: Sun Yong-gang , Li Bi-le , Ding Qing-feng , Qu Yuan , Wang Cheng-ku , Wang Lin-lin , Xu Qing-lin
Published: June 2020

The Fukeshan Cu (Mo) deposit is a newfound porphyry deposit in the northern Great Xing’an Range (GXR), northeast China. In this paper, we present results of chalcopyrite Re–Os geochronology, microthermometry of the fluid inclusions (FIs), and isotopic (H–O–S–Pb) compositions of the Fukeshan Cu (Mo) deposit. Its ore-forming process can be divided into sulfide-barren quartz veins (A vein; stage I), quartz + chalcopyrite + pyrite veins (B vein; stage II), quartz + polymetallic sulfide veins (D vein; stage III), and barren quartz + carbonate ± pyrite veins (E vein; stage IV), with Cu mineralization mainly occurred in stage II. Three types of FIs are identified in this deposit: liquid-rich two-phase (L-type) FIs, vapor-rich two-phase (V-type) FIs, daughter mineral-bearing three-phase (S-type) FIs. The homogenization temperatures of primary FIs hosted in quartz of stages I–IV are 381–494 °C, 282–398 °C, 233–340 °C, and 144–239 °C, with salinities of 7.2–58.6, 4.8–9.9, 1.4–7.9, and 0.9–3.9 wt. % NaCl equivalent, respectively. FIs microthermometry and H–O isotope data suggest that the ore-forming fluids were magmatic in origin and were gradually mixed with meteoric water from stages II to IV. Sulfur and lead isotope results indicate that the ore-forming materials of the Fukeshan Cu (Mo) deposit were likely to have originated from Late Jurassic intrusive rocks. The available data suggest that fluid cooling and incursions of meteoric water into the magmatic fluids were two important factors for Cu precipitation in the Fukeshan Cu (Mo) deposit. Chalcopyrite Re–Os dating yielded an isochron age of 144.7 ± 5.4 Ma, which is similar to the zircon U–Pb age of the quartz diorite porphyry, indicating that Late Jurassic quartz diorite porphyry and Cu mineralization occurred contemporaneously.
The Effect of Growth Medium Strength on Minimum Inhibitory Concentrations of Tannins and Tannin Extracts against E. coli
Authors: Štumpf Sara , Hostnik Gregor , Primožič Mateja , Leitgeb Maja , Salminen Juha-Pekka , Bren Urban
Published: June 2020

In this study the effect of growth medium strength on the minimum inhibitory concentration (MIC) of different tannins and tannin extracts against Escherichia coli was systematically investigated for the first time. Three pure compounds (vescalagin, castalagin and gallic acid) and five extracts (chestnut, quebracho, mimosa, Colistizer and tannic acid) were studied. Broth microdilution was assayed and bacteria were grown using different growth medium strengths varying from half to double the concentration recommended by the producer. MICs were determined using the iodonitrotetrazolium chloride (INT) dye or turbidity measurements. It was observed that MIC values depend on the growth medium strength. With an increase in the growth medium concentration MIC values rose roughly linearly for all samples, while their relative order remained unchanged, indicating that a direct interaction of tannins with growth medium nutrients represents the likely source of their antimicrobial activity. Understanding the effect of growth medium strength can finally yield a plausible explanation for the observed variation in MIC values reported in the scientific literature as well as provide help in planning proper applications of tannins in the livestock production.
Potentially Bioaccessible Phenolics from Mung Bean and Adzuki Bean Sprouts Enriched with Probiotic—Antioxidant Properties and Effect on the Motility and Survival of AGS Human Gastric Carcinoma Cells
Authors: Świeca Michał , Herok Anna , Piwowarczyk Katarzyna , Sikora Małgorzata , Ostanek Patryk , Gawlik-Dziki Urszula , Kapusta Ireneusz , Czyż Jarosław
Published: June 2020

Gastric digests from mung (MBS) and adzuki (ABS) bean sprouts enriched with probiotic Lactobacillus plantarum 299v were tested for their antioxidant potential, as well as antiproliferative and antimotility properties, in human stomach cancer cells (AGS). The digest of ABS contained quercetin and kaempferol derivates, while kaempferol and apigenin derivates were dominant in MBS. Compared to the controls, the probiotic-rich sprouts had a higher antioxidant potential—by 13% and 9%, respectively. Adzuki bean sprouts decreased the viability of AGS already at low concentrations (25% motility inhibitions). MBS and ABS displayed dose-independent cytostatic effects. The ABS extracts decreased the proliferation of AGS more effectively than the MBS extracts—0.2‰ ABS exerted c.a. 70% of inhibitions. Moreover, the phytochemicals from the probiotic-rich sprouts considerably reduced this activity. The increased vinculin level, the apoptotic shape of cell nuclei, and the reduced cell motility and proliferation indicate that the extracts exhibited cytostatic and cytotoxic activity.
Design, Preparation and Thermal Characterization of Polystyrene Composites Reinforced with Novel Three-Cages POSS Molecules
Authors: Blanco Ignazio , Bottino Francesco Agatino , Cicala Gianluca , Ognibene Giulia , Tosto Claudio
Published: June 2020

Novel polystyrene (PS)/polyhedral oligomeric silsequioxanes (POSSs) nanocomposites were designed and prepared by in situ polymerization, using, for the first time, three-cage POSS molecules. The synthesized compounds were first characterized by Fourier transform infrared spectroscopy (FTIR) and 1H NMR spectroscopy to verify the obtaining of the designed products before their thermal performance was evaluated and compared with those of pristine PS and the corresponding single-cage POSSs nanocomposites. The thermal behaviour was checked by the means of the differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). Scanning electron microscopy (SEM) was also used to confirm the hypothesis about the dispersion/aggregation of the POSS molecules into the polymer matrix. The parameters chosen to evaluate the thermal stability of the investigated compounds, namely temperature at 5% of mass loss (T5%) and solid residue at 700 °C, showed a significant increase in the stability of the polymers reinforced with the three-cages POSS, in comparison to both PS and single-cage POSS reinforced PSs, which therefore turn out to be promising molecular fillers for nanocomposite production.
Two Beta-Phosphorylamide Compounds as Ligands for Sm3+, Eu3+, and Tb3+: X-ray Crystallography and Luminescence Properties
Authors: Lear Alan R. , Lenters Jonah , Patterson Michael G. , Staples Richard J. , Werner Eric J. , Biros Shannon M.
Published: June 2020

This paper describes the synthesis of two beta-phosphorylamide ligands and their coordination chemistry with the Ln ions Tb3+, Eu3+, and Sm3+. Both the ligands and Ln complexes were characterized by IR, NMR, MS, and X-ray crystallography. The luminescence properties of the Tb3+ and Eu3+ complexes were also characterized, including the acquisition of lifetime decay curves. In the solid state, the Tb3+ and Sm3+ ligand complexes were found to have a 2:2 stoichiometry when analyzed by X-ray diffraction. In these structures, the Ln ion was bound by both oxygen atoms of each beta-phosphorylamide moiety of the ligands. The Tb3+ and Eu3+ complexes were modestly emissive as solutions in acetonitrile, with lifetime values that fell within typical ranges.
Biological Activity of Porcine Gastric Mucin on Stress Resistance and Immunomodulation
Authors: Liyanage Thiloma D. , Dahanayake Pasan S. , Edirisinghe Shan L. , Nikapitiya Chamilani , Heo Gang-Joon , De Zoysa Mahanama , Whang Ilson
Published: June 2020

Purified porcine gastric mucin (PGM) is an alternative biomaterial to native mucin which displays multifunctional properties for exploring a wide range of biomedical applications. The present study evaluated the in vitro (RAW 264.7 macrophage cells) and in vivo (zebrafish embryos and larvae) bioactivities of PGM. The median lethal concentration (LC50) of PGM was 197.9 µg/mL for embryos, while it was non-toxic to RAW 264.7 cells, even at 500 µg/mL. Following PGM exposure (100 µg/mL), a higher embryo hatching rate (59.9%) was observed at 48 h post fertilization, compared to the control (30.6%). Protective effects of PGM from pathogenic Aeromonas hydrophila were demonstrated by high larvae survival rates of 85.0% and 94.0% at 50 and 100 μg/mL of PGM exposure, respectively. Heat tolerance effect of PGM (50 and 100 µg/mL) on larvae (40 °C for 48 h) was confirmed by 75% and 100% of survival rates, respectively. Additionally, PGM reduced the A. hydrophila–induced reactive oxygen species (ROS) generation in larvae. The qRT-PCR results in PGM exposed larvae exhibited induction of immune-related genes (tlr5a and tlr5b, myd88, c-rel, il1β, tnf-α, il6, il10, cxcl18b, ccl34a.4, defbl1, hamp, ctsd, muc2.1, muc5.1, muc5.2, and muc5.3), stress response (hsp70, hsp90aa1.1, and hsp90ab1), and antioxidant genes (cat and sod1). Moreover, our results revealed that PGM involved in the regulation of transcriptional gene induction increases Hsp90 protein in the zebrafish larvae. Furthermore, upregulation of Il6, Il10, Tnfα, Ccl3, Defa-rs2, Defa21 and Camp and antioxidant genes (Sod2 and Cat) were observed in PGM-exposed RAW 264.7 cells. Overall findings confirmed the activation of immune responses, disease resistance against pathogenic bacteria, heat tolerance, and ROS-scavenging properties by PGM, which may provide insights into new applications for PGM as a multifunctional immunomodulator.
Application of Ion Mobility Spectrometry for Permeability Studies of Organic Substances through Polymeric Materials
Authors: Wiśnik-Sawka Monika , Budzyńska Edyta , Puton Jarosław
Published: June 2020

Drift tube ion mobility spectrometers (DT IMS) allow the concentration of different organic compounds to be measured. This gives the opportunity to use these detectors in measuring the penetration of various substances through polymer membranes. Permeation measurements of two substances (2-heptanone and dimethyl methylphosphonate (DMMP)) through a cylindrical silicone rubber membrane were carried out. The membrane separated the aqueous solution from the air. The analyte was introduced into water, and then its concentration in air on the opposite side of the membrane was recorded. Based on the dynamics of detector signal changes, the diffusion coefficients for both tested substances were determined. Determination of permeability coefficients was based on precise quantitative measurements, which took into account the non-linearity of the detector characteristics and the effect of water on detection sensitivity. The analysis of measurement results was based on a mathematical description of diffusion process.
Curcumin Containing PEGylated Solid Lipid Nanoparticles for Systemic Administration: A Preliminary Study
Authors: Santonocito Debora , Sarpietro Maria Grazia , Carbone Claudia , Panico Annamaria , Campisi Agata , Siciliano Edy Angela , Sposito Giovanni , Castelli Francesco , Puglia Carmelo
Published: June 2020

Curcumin (CUR) has a wide range of pharmacological properties, including anti-inflammatory and antioxidant activities, and it can be considered a good candidate for the potential treatment of central nervous system (CNS) pathologies, although its use in clinical practice is compromised due to its high lipophilicity. Solid lipid nanoparticles (SLNs) are well-known nanocarriers representing a consolidated approach for the delivery of lipophilic compounds, but their systemic use is limited due their short half-life. The formulation of stealth SLNs (pSLNs) could be a valid strategy to overcome this limit. Curcumin-loaded-pSLNs were prepared by the solvent evaporation method. Formulation was characterized for their mean size, zeta potential, size distribution, and morphology. Drug antioxidant activity was evaluated by Oxygen Radical Absorbance Capacity (ORAC) assay. Finally, the obtained formulations were analyzed in terms of long-term stability. Curcumin-loaded-pSLNs showed good technological parameters with a mean particle size below 200 nm, as confirmed by TEM images, and a zeta potential value around −30 mV, predicting good long-term stability. Differential Scanning Calorimetry (DSC) analysis confirmed that PEG micelles interacted with the SLN surface; this suggests the location of the PEG on the pSLN surface. Therefore, these preliminary studies suggest that the produced formulation could be regarded as a promising carrier for the systemic administration.
Anti-Inflammatory Principles from Tamarix aphylla L.: A Bioassay-Guided Fractionation Study
Authors: Gadallah Adel S. , Mujeeb-ur-Rehman  , Atta-ur-Rahman  , Yousuf Sammer , Atia-tul-Wahab  , Jabeen Almas , Swilam Mahmoud M. , Khalifa Shaden A. M. , El-Seedi Hesham R. , Choudhary M. Iqbal
Published: June 2020

Natural products have served as primary remedies since ancient times due to their cultural acceptance and outstanding biodiversity. To investigate whether Tamarix aphylla L. modulates an inflammatory process, we carried out bioassay-guided isolation where the extracts and isolated compounds were tested for their modulatory effects on several inflammatory indicators, such as nitric oxide (NO), reactive oxygen species (ROS), proinflammatory cytokine; tumour necrosis factor (TNF-α), as well as the proliferation of the lymphocyte T-cells. The aqueous ethanolic extract of the plant inhibited the intracellular ROS production, NO generation, and T-cell proliferation. The aqueous ethanolic crude extract was partitioned by liquid-liquid fractionation using n-hexane (n-C6H6), dichloromethane (DCM), ethyl acetate (EtOAc), n-butanol (n-BuOH), and water (H2O). The DCM and n-BuOH extracts showed the highest activity against most inflammatory indicators and were further purified to obtain compounds 1–4. The structures of 3,5-dihydroxy-4’,7-dimethoxyflavone (1) and 3,5-dihydroxy-4-methoxybenzoic acid methyl ester (2) from the DCM extracts; and kaempferol (3), and 3-hydroxy-4-methoxy-(E)-cinnamic acid (4) from the n-BuOH extract were elucidated by different spectroscopic tools, including MS, NMR, UV, and IR. Compound 2 inhibited the production of ROS and TNF-α, whereas compound 3 showed inhibitory activity against all the tested mediators. A better understanding of the potential aspect of Tamarix aphylla L. derivatives as anti-inflammatory agents could open the door for the development of advanced anti-inflammatory entities.
Concentrated Bioshell Calcium Oxide (BiSCaO) Water Kills Pathogenic Microbes: Characterization and Activity
Authors: Nakamura Shingo , Ishihara Masayuki , Sato Yoko , Takayama Tomohiro , Hiruma Sumiyo , Ando Naoko , Fukuda Koichi , Murakami Kaoru , Yokoe Hidetaka
Published: June 2020

Bioshell calcium oxide (BiSCaO) exhibits deodorizing properties and broad microbicidal activity. In this study, we examined possible utility of BiSCaO Water for that purpose. BiSCaO Water was prepared by adding 10 wt% BiSCaO to clean water and gently collecting the supernatant in a bottle. The same volume of clean water was gently poured onto the BiSCaO precipitate and the supernatant was gently collected in a bottle; this process was repeated fifty times. The produced BiSCaO Water contained nanoparticles (about 400–800 nm) composed of smaller nanoparticles (100–200 nm), and was colorless and transparent, with a pH > 12.7. In vitro assays demonstrated that BiSCaO Water eliminated more than 99.9% of influenza A (H1N1) and Feline calicivirus, Escherichia coli such as NBRC 3972 and O-157:H7, Pseudomonas aeruginosa, Salmonella, and Staphylococcus aureus within 15 min. We compared BiSCaO Water with the other microbicidal reagents such as ethanol, BiSCaO, BiSCa(OH)2 suspensions, povidone iodine, NaClO, BiSCaO dispersion and colloidal dispersion with respect to deodorization activity and microbicidal efficacy. The results showed that BiSCaO Water was a potent reagent with excellent deodorization and disinfection activities against pathogenic bacteria and viruses (including both enveloped and nonenveloped viruses).
Charge Accumulation of Amplified Spontaneous Emission in a Conjugated Polymer Chain and Its Dynamical Phonon Spectra
Authors: Lin Zhe , Chen Jiahao , Zhang Yusong , Shen Jianguo , Li Sheng , George Thomas F.
Published: June 2020

In this article, the detailed photoexcitation dynamics which combines nonadiabatic molecular dynamics with electronic transitions shows the occurrence of amplified spontaneous emission (ASE) in conjugated polymers, accompanied by spontaneous electric polarization. The elaborate molecular dynamic process of ultrafast photoexcitation can be described as follows: Continuous external optical pumping (laser of 70 µJ/cm2) not only triggers the appearance of an instantaneous four-level electronic structure but causes population inversion for ASE as well. At the same time, the phonon spectrum of the conjugated polymer changes, and five local infrared lattice vibrational modes form at the two ends, which break the original symmetry in the system and leads to charge accumulation at the ends of the polymer chain without an external electric field. This novel phenomenon gives a brand-new avenue to explain how the lattice vibrations play a role in the evolution of the stimulated emission, which leads to an ultrafast effect in solid conjugated polymers.
LC/MS Analysis of Saponin Fraction from the Leaves of Elaeagnus rhamnoides (L.) A. Nelson and Its Biological Properties in Different In Vitro Models
Authors: Żuchowski Jerzy , Skalski Bartosz , Juszczak Michał , Woźniak Katarzyna , Stochmal Anna , Olas Beata
Published: June 2020

This study focuses on saponin fraction from sea buckthorn (Elaeagnus rhamnoides (L.) A. Nelson) leaves. It has known that for example teas from sea buckthorn leaves have anti-obesity properties. The objective of our present experiments was to investigate both the chemical composition of saponin fraction, as well as their biological properties in different in vitro models (using human plasma, blood platelets, and peripheral blood mononuclear cells (PBMCs)). We observed that saponin fraction reduces plasma lipid peroxidation and protein carbonylation induced by H2O2/Fe. This fraction also decreased DNA oxidative damage induced by H2O2 in PBMCs. Regarding the cytotoxicity of saponin fraction (0.5–50 µg/mL) none was found to cause lysis of blood platelets, and PBMCs. Our results, for the first time indicate that saponin fraction from sea buckthorn leaves may be a new promising source of compounds for prophylaxis and treatment of diseases associated with oxidative stress.
Analysis of Iron Oxide Reduction Kinetics in the Nanometric Scale Using Hydrogen
Authors: Manchili Swathi K. , Wendel Johan , Hryha Eduard , Nyborg Lars
Published: June 2020

Iron nanopowder could be used as a sintering aid to water-atomised steel powder to improve the sintered density of metallurgical (PM) compacts. For the sintering process to be efficient, the inevitable surface oxide on the nanopowder must be reduced at least in part to facilitate its sintering aid effect. While appreciable research has been conducted in the domain of oxide reduction of the normal ferrous powder, the same cannot be said about the nanometric counterpart. The reaction kinetics for the reduction of surface oxide of iron nanopowder in hydrogen was therefore investigated using nonisothermal thermogravimetric (TG) measurements. The activation energy values were determined from the TG data using both isoconversional Kissinger–Akahira–Sunose (KAS) method and the Kissinger approach. The values obtained were well within the range of reported data. The reaction kinetics of Fe2O3 as a reference material was also depicted and the reduction of this oxide proceeds in two sequential stages. The first stage corresponds to the reduction of Fe2O3 to Fe3O4, while the second stage corresponds to a complete reduction of oxide to metallic Fe. The activation energy variation over the reduction process was observed and a model was proposed to understand the reduction of surface iron oxide of iron nanopowder.
Application of a Micro Free-Flow Electrophoresis 3D Printed Lab-on-a-Chip for Micro-Nanoparticles Analysis
Authors: Barbaresco Federica , Cocuzza Matteo , Pirri Candido Fabrizio , Marasso Simone Luigi
Published: June 2020

The present work describes a novel microfluidic free-flow electrophoresis device developed by applying three-dimensional (3D) printing technology to rapid prototype a low-cost chip for micro- and nanoparticle collection and analysis. Accurate reproducibility of the device design and the integration of the inlet and outlet ports with the proper tube interconnection was achieved by the additive manufacturing process. Test prints were performed to compare the glossy and the matte type of surface finish. Analyzing the surface topography of the 3D printed device, we demonstrated how the best reproducibility was obtained with the glossy device showing a 5% accuracy. The performance of the device was demonstrated by a free-flow zone electrophoresis application on micro- and nanoparticles with different dimensions, charge surfaces and fluorescent dyes by applying different separation voltages up to 55 V. Dynamic light scattering (DLS) measurements and ultraviolet−visible spectroscopy (UV−Vis) analysis were performed on particles collected at the outlets. The percentage of particles observed at each outlet was determined in order to demonstrate the capability of the micro free-flow electrophoresis (µFFE) device to work properly in dependence of the applied electric field. In conclusion, we rapid prototyped a microfluidic device by 3D printing, which ensured micro- and nanoparticle deviation and concentration in a reduced operation volume and hence suitable for biomedical as well as pharmaceutical applications.
Magnetocrystalline and Surface Anisotropy in CoFe2O4 Nanoparticles
Authors: Omelyanchik Alexander , Salvador María , D’Orazio Franco , Mameli Valentina , Cannas Carla , Fiorani Dino , Musinu Anna , Rivas Montserrat , Rodionova Valeria , Varvaro Gaspare , Peddis Davide
Published: June 2020

The effect of the annealing temperature Tann on the magnetic properties of cobalt ferrite nanoparticles embedded in an amorphous silica matrix (CoFe2O4/SiO2), synthesized by a sol-gel auto-combustion method, was investigated by magnetization and AC susceptibility measurements. For samples with 15% w/w nanoparticle concentration, the particle size increases from ~2.5 to ~7 nm, increasing Tann from 700 to 900 °C. The effective magnetic anisotropy constant (Keff) increases with decreasing Tann, due to the increase in the surface contribution. For a 5% w/w sample annealed at 900 °C, Keff is much larger (1.7 × 106 J/m3) than that of the 15% w/w sample (7.5 × 105 J/m3) annealed at 700 °C and showing comparable particle size. This indicates that the effect of the annealing temperature on the anisotropy is not only the control of the particle size but also on the core structure (i.e., cation distribution between the two spinel sublattices and degree of spin canting), strongly affecting the magnetocrystalline anisotropy. The results provide evidence that the magnetic anisotropy comes from a complex balance between core and surface contributions that can be controlled by thermal treatments.
LncRNAs in Non-Small-Cell Lung Cancer
Authors: Ginn Lucy , Shi Lei , La Montagna Manuela , Garofalo Michela
Published: June 2020

Lung cancer is associated with a high mortality, with around 1.8 million deaths worldwide in 2018. Non-small-cell lung cancer (NSCLC) accounts for around 85% of cases and, despite improvement in the management of NSCLC, most patients are diagnosed at advanced stage and the five-year survival remains around 15%. This highlights a need to identify novel ways to treat the disease to reduce the burden of NSCLC. Long non-coding RNAs (lncRNAs) are non-coding RNA molecules longer than 200 nucleotides in length which play important roles in gene expression and signaling pathways. Recently, lncRNAs were implicated in cancer, where their expression is dysregulated resulting in aberrant functions. LncRNAs were shown to function as both tumor suppressors and oncogenes in a variety of cancer types. Although there are a few well characterized lncRNAs in NSCLC, many lncRNAs remain un-characterized and their mechanisms of action largely unknown. LncRNAs have success as therapies in neurodegenerative diseases, and having a detailed understanding of their function in NSCLC may guide novel therapeutic approaches and strategies. This review discusses the role of lncRNAs in NSCLC tumorigenesis, highlighting their mechanisms of action and their clinical potential.
The Effect of Vitamin D3 Supplementation on Physical Capacity among Active College-Aged Males
Authors: Kujach Sylwester , Lyzwinski Dariusz , Chroboczek Maciej , Bialowas Dawid , Antosiewicz Jedrzej , Laskowski Radoslaw
Published: June 2020

Vitamin D3 supplementation can affect strength and power; however, the effect on both aerobic and anaerobic performance remains unclear. Here, we investigate the effects of eight weeks of a high dose of vitamin D3 supplementation and its impact on circulating 25-hydroxyvitamin D (25-OH-D3) concentrations and selected indicators of physical capacity. Subjects (n = 28, age 21.1 ± 1.6) were divided into two groups: supplemented (SUP), which was given 6000 IU of vitamin D3 daily for eight weeks; and placebo group (PLA). Serum 25-OH-D3 concentrations were determined in pre- and post-intervention. Aerobic ( V . O2max test) and anaerobic (Wingate Anaerobic Test) capacity were determined before and after the supplementation. The mean baseline concentration of 25-OH-D3 was recognized as deficient (20 ng/mL) and significantly increased over time in the supplemented group (p < 0.01, η2 = 0.86), whilst it remained unchanged in the placebo group. Moreover, the supplementation caused a significant improvement in maximal aerobic (p < 0.05, η2 = 0.27) and anaerobic power (p < 0.01, η2 = 0.51) whereas no changes were observed in PLA group. The V . O2max differences were also significant in the supplemented group (p < 0.05). In summary, the changes in aerobic and anaerobic capacity observed in this study were associated with a serum concentration of 25-OH-D3. Our data imply that vitamin D3 supplementation with a dose of 6000 IU daily for eight weeks is sufficient to improve physical capacity and vitamin D3 status.
Serum 25-Hydroxyvitamin D Concentrations Are Associated with Mental Health and Psychosocial Stress in Young Adults
Authors: Chen Li , Zhu Haidong , Harshfield Gregory A. , Treiber Frank A. , Pollock Jennifer S. , Pollock David , Okereke Olivia I. , Su Shaoyong , Dong Yanbin
Published: June 2020

We aimed to test the hypothesis that serum 25-hydroxyvitamin D3 (25(OH)D) concentration is associated with mental health and life stress measures in young adults and investigate gender and racial disparities in these associations. This study comprised 327 black and white participants. Depression, trait anxiety, perceived stress, and hostility were measured by the following validated instruments: Beck Depression Inventory (BDI), State-Trait Anxiety Inventory (STAI), Perceived Stress Scale (PSS), and Cook–Medley Hostility Scale (CMHS). Linear regression was used to estimate correlations between serum 25(OH)D concentration and mental health measurements in the total population and in subgroups stratified by gender and race. In this sample (28.2 ± 3.1 years, 52% female, 53% black), serum 25(OH)D concentration was negatively related to BDI, STAI, PSS, total CMHS score, and the majority of CMHS subscale scores (p-values < 0.05). Stratified by gender, most of these associations remained significant only in women (p-values < 0.05). Stratified by race, higher 25(OH)D concentrations in white participants were significantly related to lower BDI, STAI, PSS, and CMHS-cynicism subscales (p-values < 0.05); 25(OH)D concentrations in the black participants were only inversely associated with CMHS and most CMHS subscales (p-values < 0.05) but not with BDI, STAI, and PSS. We present novel findings of consistent inverse relationships between serum 25(OH)D concentration and various measures of mental health and life stress. Long-term interventional studies are warranted in order to investigate the roles of vitamin D supplementation in the prevention and mitigation of depression, anxiety, and psychological stress in young adults.