bluuebunny/crossref_metadata_2025 huggingface dataset:
Review on Cardiorespiratory Complications after SARS-CoV-2 Infection in Young Adult Healthy Athletes
Authors: Romagnoli Sofia , Sbrollini Agnese , Marcantoni Ilaria , Morettini Micaela , Burattini Laura
Published: May 2022

This review analyzes scientific data published in the first two years of the COVID-19 pandemic with the aim to report the cardiorespiratory complications observed after SARS-CoV-2 infection in young adult healthy athletes. Fifteen studies were selected using PRISMA guidelines. A total of 4725 athletes (3438 males and 1287 females) practicing 19 sports categories were included in the study. Information about symptoms was released by 4379 (93%) athletes; of them, 1433 (33%) declared to be asymptomatic, whereas the remaining 2946 (67%) reported the occurrence of symptoms with mild (1315; 45%), moderate (821; 28%), severe (1; 0%) and unknown (809; 27%) severity. The most common symptoms were anosmia (33%), ageusia (32%) and headache (30%). Cardiac magnetic resonance identified the largest number of cardiorespiratory abnormalities (15.7%). Among the confirmed inflammations, myocarditis was the most common (0.5%). In conclusion, the low degree of symptom severity and the low rate of cardiac abnormalities suggest that the risk of significant cardiorespiratory involvement after SARS-CoV-2 infection in young adult athletes is likely low; however, the long-term physiologic effects of SARS-CoV-2 infection are not established yet. Extensive cardiorespiratory screening seems excessive in most cases, and classical pre-participation cardiovascular screening may be sufficient.
Digital Soil Mapping of Soil Organic Matter with Deep Learning Algorithms
Authors: Zeng Pengyuan , Song Xuan , Yang Huan , Wei Ning , Du Liping
Published: May 2022

Digital soil mapping has emerged as a new method to describe the spatial distribution of soils economically and efficiently. In this study, a lightweight soil organic matter (SOM) mapping method based on a deep residual network, which we call LSM-ResNet, is proposed to make accurate predictions with background covariates. ResNet not only integrates spatial background information around the observed environmental covariates, but also reduces problems such as information loss, which undermines the integrity of information and reduces prediction uncertainty. To train the model, rectified linear units, mean squared error, and adaptive momentum estimation were used as the activation function, loss/cost function, and optimizer, respectively. The method was tested with Landsat5, the meteorological data from WorldClim, and the 1602 sampling points set from Xinxiang, China. The performance of the proposed LSM-ResNet was compared to a traditional machine learning algorithm, the random forest (RF) algorithm, and a training set (80%) and a test set (20%) were created to test both models. The results showed that the LSM-ResNet (RMSE = 6.40, R2 = 0.51) model outperformed the RF model in both the roots mean square error (RMSE) and coefficient of determination (R2), and the training accuracy was significantly improved compared to RF (RMSE = 6.81, R2 = 0.46). The trained LSM-ResNet model was used for SOM prediction in Xinxiang, a district of plain terrain in China. The prediction maps can be deemed an accurate reflection of the spatial variability of the SOM distribution.
Continuous Ingestion of Lacticaseibacillus rhamnosus JB-1 during Chronic Stress Ensures Neurometabolic and Behavioural Stability in Rats
Authors: Chudzik Agata , Słowik Tymoteusz , Kochalska Katarzyna , Pankowska Anna , Łazorczyk Artur , Andres-Mach Marta , Rola Radosław , Stanisz Greg J. , Orzyłowska Anna
Published: May 2022

The intestinal microbiome composition and dietary supplementation with psychobiotics can result in neurochemical alterations in the brain, which are possible due to the presence of the brain–gut–microbiome axis. In the present study, magnetic resonance spectroscopy (MRS) and behavioural testing were used to evaluate whether treatment with Lacticaseibacillus rhamnosus JB-1 (JB‑1) bacteria alters brain metabolites’ levels and behaviour during continuous exposure to chronic stress. Twenty Wistar rats were subjected to eight weeks of a chronic unpredictable mild stress protocol. Simultaneously, half of them were fed with JB-1 bacteria, and the second half was given a daily placebo. Animals were examined at three-time points: before starting the stress protocol and after five and eight weeks of stress onset. In the elevated plus maze behavioural test the placebo group displayed increased anxiety expressed by almost complete avoidance of exploration, while the JB-1 dietary supplementation mitigated anxiety which resulted in a longer exploration time. Hippocampal MRS measurements demonstrated a significant decrease in glutamine + glutathione concentration in the placebo group compared to the JB-1 bacteria-supplemented group after five weeks of stress. With the progression of stress the decrease of glutamate, glutathione, taurine, and macromolecular concentrations were observed in the placebo group as compared to baseline. The level of brain metabolites in the JB-1-supplemented rats were stable throughout the experiment, with only the taurine level decreasing between weeks five and eight of stress. These data indicated that the JB-1 bacteria diet might stabilize levels of stress-related neurometabolites in rat brain and could prevent the development of anxiety/depressive-like behaviour.
Immunoglobulin G N-glycan Biomarkers for Autoimmune Diseases: Current State and a Glycoinformatics Perspective
Authors: Flevaris Konstantinos , Kontoravdi Cleo
Published: May 2022

The effective treatment of autoimmune disorders can greatly benefit from disease-specific biomarkers that are functionally involved in immune system regulation and can be collected through minimally invasive procedures. In this regard, human serum IgG N-glycans are promising for uncovering disease predisposition and monitoring progression, and for the identification of specific molecular targets for advanced therapies. In particular, the IgG N-glycome in diseased tissues is considered to be disease-dependent; thus, specific glycan structures may be involved in the pathophysiology of autoimmune diseases. This study provides a critical overview of the literature on human IgG N-glycomics, with a focus on the identification of disease-specific glycan alterations. In order to expedite the establishment of clinically-relevant N-glycan biomarkers, the employment of advanced computational tools for the interpretation of clinical data and their relationship with the underlying molecular mechanisms may be critical. Glycoinformatics tools, including artificial intelligence and systems glycobiology approaches, are reviewed for their potential to provide insight into patient stratification and disease etiology. Challenges in the integration of such glycoinformatics approaches in N-glycan biomarker research are critically discussed.
Skin Inflammation Modulation via TNF-α, IL-17, and IL-12 Family Inhibitors Therapy and Cancer Control in Patients with Psoriasis
Authors: Costache Daniel Octavian , Feroiu Oana , Ghilencea Adelina , Georgescu Mihaela , Căruntu Ana , Căruntu Constantin , Țiplica Sorin George , Jinga Mariana , Costache Raluca Simona
Published: May 2022

The systemic inflammatory syndrome concept is one of the foundations that stand at the basis of revolutionary modern and future therapies, based on the in-depth understanding of the delicate mechanisms that govern the collaboration between the systems and organs of the human body and, at the same time, the fine balance that ensures a reproach-free operation. An interesting concept that we propose is that of the environment-inadequacy status, a concept that non-specifically incorporates all the situations of the organism’s response disorders in the face of imprecisely defined situations of the environment. The correlation between these two concepts will define the future of modern medicine, along with the gene-adjustment mechanisms. Psoriasis is a clear example of an inadequate body response as a result of exposure to as of yet undefined triggers with an excessive systemic inflammatory reaction and hitherto insufficiently controllable. Modern biological therapies, such as TNF-α, IL-12 family, and IL-17 inhibitors, are intended to profoundly reshape the cytokine configuration of patients with inflammatory diseases such as psoriasis, with tremendous success in disease control. Yet, because of the important roles of cytokines in cancer promotion and control, concern was raised about the fact that the use of biologicals may alter immune surveillance and promote cancer progression. Both theoretical and practical data nevertheless showed that the treatment-induced control of cytokines may be beneficial for reducing the inflammatory milieu that promotes cancer and such have a beneficial role in maintaining health. We briefly present the intricate roles of those cytokine families on cancer control, with some debates on if their inhibition might or might not promote additional tumoral development.
Genome-Wide Identification and Expression of MAPK Gene Family in Cultivated Strawberry and Their Involvement in Fruit Developing and Ripening
Authors: Li Mengyao , Li Binghua , Yang Min , Wang Liangxin , Hou Guoyan , Lin Yuanxiu , Zhang Yunting , Zhang Yong , Chen Qing , Wang Yan , He Wen , Wang Xiaorong , Tang Haoru , Yang Guichuan , Luo Ya
Published: May 2022

Studies on many plants have shown that mitogen-activated protein kinases (MAPKs) are key proteins involved in regulating plant responses to biotic and abiotic stresses. However, their involvement in cultivated strawberry development and ripening remains unclear. In this study, 43 FaMAPK gene family members were identified in the genome of cultivated strawberry (Fragaria × ananassa), phylogenetic analysis indicated that FaMAPKs could be classified into four groups. Systematic analysis of the conserved motif, exon–intron structure showed that there were significant varieties between different groups in structure, but in the same group they were similar. Multiple cis-regulatory elements associated with phytohormone response, and abiotic and biotic stresses were predicted in the promoter regions of FaMAPK genes. Transcriptional analysis showed that all FaMAPK genes were expressed at all developmental stages. Meanwhile, the effect of exogenous ABA and sucrose on the expression profile of FaMAPKs was investigated. Exogenous ABA, sucrose, and ABA plus sucrose treatments upregulated the expression of FaMAPK genes and increased the content of endogenous ABA, sucrose, and anthocyanin in strawberry fruits, suggesting that ABA and sucrose might be involved in the FaMAPK-mediated regulation of strawberry fruit ripening. Based on the obtained results, MAPK genes closely related to the ripening of strawberries were screened to provide a theoretical basis and support for future research on strawberries.
Polysialylation in a DISC1 Mutant Mouse
Authors: Takahashi Yuka , Abe Chikara , Hane Masaya , Wu Di , Kitajima Ken , Sato Chihiro
Published: May 2022

Schizophrenia is a serious psychiatric disorder that affects the social life of patients. Psychiatric disorders are caused by a complex combination of genetic (G) and environmental (E) factors. Polysialylation represents a unique posttranslational modification of a protein, and such changes in neural cell adhesion molecules (NCAMs) have been reported in postmortem brains from patients with psychiatric disorders. To understand the G × E effect on polysialylated NCAM expression, in this study, we performed precise measurements of polySia and NCAM using a disrupted-in-schizophrenia 1 (DISC1)-mutant mouse (G), a mouse model of schizophrenia, under acute stress conditions (E). This is the first study to reveal a lower number and smaller length of polySia in the suprachiasmatic nucleus of DISC1 mutants relative to those in wild-type (WT) mice. In addition, an analysis of polySia and NCAM responses to acute stress in five brain regions (olfactory bulb, prefrontal cortex, suprachiasmatic nucleus, amygdala, and hippocampus) revealed that the pattern of changes in these responses in WT mice and DISC1 mutants differed by region. These differences could indicate the vulnerability of DISC1 mutants to stress.
Fluorescent Imaging of Extracellular Fungal Enzymes Bound onto Plant Cell Walls
Authors: Gacias-Amengual Neus , Wohlschlager Lena , Csarman Florian , Ludwig Roland
Published: May 2022

Lignocelluloytic enzymes are industrially applied as biocatalysts for the deconstruction of recalcitrant plant biomass. To study their biocatalytic and physiological function, the assessment of their binding behavior and spatial distribution on lignocellulosic material is a crucial prerequisite. In this study, selected hydrolases and oxidoreductases from the white rot fungus Phanerochaete chrysosporium were localized on model substrates as well as poplar wood by confocal laser scanning microscopy. Two different detection approaches were investigated: direct tagging of the enzymes and tagging specific antibodies generated against the enzymes. Site-directed mutagenesis was employed to introduce a single surface-exposed cysteine residue for the maleimide site-specific conjugation. Specific polyclonal antibodies were produced against the enzymes and were labeled using N-hydroxysuccinimide (NHS) ester as a cross-linker. Both methods allowed the visualization of cell wall-bound enzymes but showed slightly different fluorescent yields. Using native poplar thin sections, we identified the innermost secondary cell wall layer as the preferential attack point for cellulose-degrading enzymes. Alkali pretreatment resulted in a partial delignification and promoted substrate accessibility and enzyme binding. The methods presented in this study are suitable for the visualization of enzymes during catalytic biomass degradation and can be further exploited for interaction studies of lignocellulolytic enzymes in biorefineries.
Can Similarities between the Pathogenesis of Preeclampsia and COVID-19 Increase the Understanding of COVID-19?
Authors: Giardini Valentina , Gambacorti-Passerini Carlo , Casati Marco , Carrer Andrea , Vergani Patrizia
Published: May 2022

COVID-19 has been primarily identified as a respiratory infection characterized by signs and symptoms associated with the dysfunction of the renin-angiotensin system (RAS). This is attributed to the SARS-CoV-2 virus invading the respiratory mucosa via angiotensin-converting enzyme 2 (ACE2), which is an important element of the RAS. Meanwhile, preeclampsia is an obstetric pathology that, surprisingly, resembles the pathology of COVID-19. It is a systemic syndrome that occurs during the second half of pregnancy and is determined to be a major cause of maternal and perinatal morbidity and mortality. This disease typically presents with new-onset hypertension and proteinuria or other specific end-organ dysfunctions. RAS-mediated mechanisms may explain its primary clinical-pathological features, which are suggestive of an underlying microvascular dysfunction in both diseases, with induction of vasculopathy, coagulopathy, and inflammation. In this report, we review the medical literature on this subject. Further, the underlying similarities between the two conditions are discussed to assess preeclampsia as a model for COVID-19. These considerations are valid in the case of original SARS-CoV-2 primary infection. Emerging SARS-CoV-2 variants as well as the vaccination could alter various aspects of the virus biology, including human ACE-2 receptor binding affinity and therefore the RAS mediated consequences.
Factors Influencing Watching and Purchase Intentions on Live Streaming Platforms: From a 7Ps Marketing Mix Perspective
Authors: Ho Chaang-Iuan , Liu Yaoyu , Chen Ming-Chih
Published: May 2022

Previous studies have investigated how customer purchase intention is influenced by live streaming. However, no study has investigated the effect of service marketing mix (7Ps) on consumer shopping behavior from sellers’ perspectives. The present study is designed to shed light on the relationships among the 7Ps and the customers’ purchase intention through watching the broadcasters’ show. An integrative marketing-oriented model is proposed and tested using data collected from 330 customers (including 237 shoppers for apparel and 93 customers for seafood) through Facebook live shopping platforms. The research results reveal that promotion, placement, and physical evidence have positive effects on customers’ purchase intention. In addition, the watching intention had a positive effect on purchase intention. It is also found that watching intention has a full mediation effect on the relationship between the 7Ps marketing mix and the purchase intention. The implications of the findings and issues for future research are also discussed.
Identification and Characterization of Genes Related to Resistance of Autographa californica Nucleopolyhedrovirus Infection in Bombyx mori
Authors: Kong Yunhui , Sun Lingling , Tang Yaling , Li Jiashuang , Qin Sheng , Li Muwang
Published: May 2022

In Bombyx mori, as an important economic insect, it was first found that some strains were completely refractory to infection with Autographa californica nucleopolyhedrovirus (AcMNPV) through intrahemocelical injection; whereas almost all natural strains had difficulty resisting Bombyx mori nucleopolyhedrovirus (BmNPV), which is also a member of the family Baculoviridae. Previous genetics analysis research found that this trait was controlled by a potentially corresponding locus on chromosome 3, but the specific gene and mechanism was still unknown. With the help of the massive silkworm strain re-sequencing dataset, we performed the Genome-Wide Association Studies (GWAS) to identify the gene related to the resistance of AcMNPV in this study. The GWAS results showed that the Niemann-Pick type C1 (NPC-1) gene was the most associated with the trait. The knockdown experiments in BmN cells showed that BmNPC1 has a successful virus suppression infection ability. We found a small number of amino acid mutations among different resistant silkworms, which indicates that these mutations contributed to the resistance of AcMNPV. Furthermore, inhibition of the BmNPC1 gene also changed the viral gene expression of the AcMNPV, which is similar to the expression profile in the transcriptome data of p50 and C108 strains.
Review of the Family Thanerocleridae (Coleoptera: Cleroidea) and the Description of Thanerosus gen. nov. from Cretaceous Amber Using Micro-CT Scanning
Authors: Peris David , Mähler Bastian , Kolibáč Jiří
Published: May 2022

The predaceous beetle family Thanerocleridae is one of the smallest families of Cleroidea. It comprises only 36 extant species widespread on all continents. Three more species have been described from Cretaceous ambers of Myanmar and France. The fourth fossil representative of Thanerocleridae is described herein. Thanerosus antiquus gen. and sp. nov. is based on one fossil specimen preserved in an amber piece from Upper Cretaceous Kachin amber. The holotype was imaged using an X-ray micro-CT system to obtain high-quality 3D images. A phylogenetic analysis based on 33 morphological characters supports the placement of the new genus at the basal position in a tree of Thanerocleridae, in the vicinity of extant Zenodosus Wolcott and three extinct Mesozoic genera with which the new fossil shares open procoxal and mesocoxal cavities and transverse procoxae. We offer here a key to all extant and extinct genera in the family together with a complete list of all valid thaneroclerid taxa.
Negative-Pressure Ventilation in Neuromuscular Diseases in the Acute Setting
Authors: Annunziata Anna , Calabrese Cecilia , Simioli Francesca , Coppola Antonietta , Flora Martina , Marotta Antonella , Di Spirito Valentina , Didonna Francesco , Cicalese Marcellino , Fiorentino Giuseppe
Published: May 2022

Mechanical ventilation started with negative-pressure ventilation (NPV) during the 1950s to assist patients with respiratory failure, secondary to poliomyelitis. Over the years, technological evolution has allowed for the development of more comfortable devices, leading to an increased interest in NPV. The patients affected by neuromuscular diseases (NMD) with chronic and acute respiratory failure (ARF) may benefit from NPV. The knowledge of the available respiratory-support techniques, indications, contraindications, and adverse effects is necessary to offer the patient a personalized treatment that considers the pathology’s complexity.
Endometrial Cancer Patient-Derived Xenograft Models: A Systematic Review
Authors: Tanaka Tomohito , Nishie Ruri , Ueda Shoko , Miyamoto Shunsuke , Hashida Sousuke , Konishi Hiromi , Terada Shinichi , Kogata Yuhei , Sasaki Hiroshi , Tsunetoh Satoshi , Taniguchi Kohei , Komura Kazumasa , Ohmichi Masahide
Published: May 2022

Background: Because patient-derived xenograft (PDX) models resemble the original tumors, they can be used as platforms to find target agents for precision medicine and to study characteristics of tumor biology such as clonal evolution and microenvironment interactions. The aim of this review was to identify articles on endometrial cancer PDXs (EC-PDXs) and verify the methodology and outcomes. Methods: We used PubMed to research and identify articles on EC-PDX. The data were analyzed descriptively. Results: Post literature review, eight studies were selected for the systematic review. Eighty-five EC-PDXs were established from 173 patients with EC, with a total success rate of 49.1%. A 1–10 mm3 fragment was usually implanted. Fresh-fragment implantation had higher success rates than using overnight-stored or frozen fragments. Primary tumors were successfully established with subcutaneous implantation, but metastasis rarely occurred; orthotopic implantation via minced tumor cell injection was better for metastatic models. The success rate did not correspond to immunodeficiency grades, and PDXs using nude mice reduced costs. The tumor growth period ranged from 2 weeks to 13 months. Similar characteristics were observed between primary tumors and PDXs, including pathological findings, gene mutations, and gene expression. Conclusion: EC-PDXs are promising tools for translational research because they closely resemble the features of tumors in patients and retain molecular and histological features of the disease.
SARS-CoV-2 Droplet and Airborne Transmission Heterogeneity
Authors: Baselga Marta , Güemes Antonio , Alba Juan J. , Schuhmacher Alberto J.
Published: May 2022

The spread dynamics of the SARS-CoV-2 virus have not yet been fully understood after two years of the pandemic. The virus’s global spread represented a unique scenario for advancing infectious disease research. Consequently, mechanistic epidemiological theories were quickly dismissed, and more attention was paid to other approaches that considered heterogeneity in the spread. One of the most critical advances in aerial pathogens transmission was the global acceptance of the airborne model, where the airway is presented as the epicenter of the spread of the disease. Although the aerodynamics and persistence of the SARS-CoV-2 virus in the air have been extensively studied, the actual probability of contagion is still unknown. In this work, the individual heterogeneity in the transmission of 22 patients infected with COVID-19 was analyzed by close contact (cough samples) and air (environmental samples). Viral RNA was detected in 2/19 cough samples from patient subgroups, with a mean Ct (Cycle Threshold in Quantitative Polymerase Chain Reaction analysis) of 25.7 ± 7.0. Nevertheless, viral RNA was only detected in air samples from 1/8 patients, with an average Ct of 25.0 ± 4.0. Viral load in cough samples ranged from 7.3 × 105 to 8.7 × 108 copies/mL among patients, while concentrations between 1.1–4.8 copies/m3 were found in air, consistent with other reports in the literature. In patients undergoing follow-up, no viral load was found (neither in coughs nor in the air) after the third day of symptoms, which could help define quarantine periods in infected individuals. In addition, it was found that the patient’s Ct should not be considered an indicator of infectiousness, since it could not be correlated with the viral load disseminated. The results of this work are in line with proposed hypotheses of superspreaders, which can attribute part of the heterogeneity of the spread to the oversized emission of a small percentage of infected people.
Chronic Pain in Musculoskeletal Diseases: Do You Know Your Enemy?
Authors: Bonanni Roberto , Cariati Ida , Tancredi Virginia , Iundusi Riccardo , Gasbarra Elena , Tarantino Umberto
Published: May 2022

Musculoskeletal pain is a condition that characterises several diseases and represents a constantly growing issue with enormous socio-economic burdens, highlighting the importance of developing treatment algorithms appropriate to the patient’s needs and effective management strategies. Indeed, the algic condition must be assessed and treated independently of the underlying pathological process since it has an extremely negative impact on the emotional and psychic aspects of the individual, leading to isolation and depression. A full understanding of the pathophysiological mechanisms involved in nociceptive stimulation and central sensitization is an important step in improving approaches to musculoskeletal pain. In this context, the bidirectional relationship between immune cells and neurons involved in nociception could represent a key point in the understanding of these mechanisms. Therefore, we provide an updated overview of the magnitude of the musculoskeletal pain problem, in terms of prevalence and costs, and summarise the role of the most important molecular players involved in the development and maintenance of pain. Finally, based on the pathophysiological mechanisms, we propose a model, called the “musculoskeletal pain cycle”, which could be a useful tool to counteract resignation to the algic condition and provide a starting point for developing a treatment algorithm for the patient with musculoskeletal pain.
Dental Treatment under General Anesthesia in Pre-School Children and Schoolchildren with Special Healthcare Needs: A Comparative Retrospective Study
Authors: Schulz-Weidner Nelly , Schlenz Maximiliane Amelie , Jung Linda Giuliana , Uebereck Constanze Friederike , Nehls Agnes , Krämer Norbert
Published: May 2022

The aim of this retrospective study was to evaluate the dental treatments received under general anesthesia (GA) in pre-school children and school children with special healthcare needs (n = 263) compared with healthy controls (n = 62). In order to analyze the impact of pre-existing cofactors on oral health, children were divided into the following groups: heart disease, cancer, syndromic disease, and other diseases as well as in- and outpatient healthy children. Caries experience (dmf-t/DMF-T) before GA (impulse 1), waiting time, and dental treatment under GA (impulse 2) were determined. Pre-school children showed a higher caries experience (mean ± standard deviation; 8.3 ± 4.8) compared to schoolchildren (6.9 ± 4.3). Predominantly, early childhood caries (ECC) of type 1 were detected. From all groups with special healthcare needs, children with cancer revealed the highest Significant Caries Index (17.0 ± 2.0), followed by other diseases (14.6 ± 2.6), syndromic disease (14.3 ± 1.8), and heart disease (13.8 ± 2.7). Overall, 2607 dental procedures were performed under GA with a mean of 8.0 ± 6.5 dental measures per child. Within the limitations of this study, the data exhibited independent of pre-existing cofactors and age high caries risk in all patient groups showing a high need for treatment.
Efficacy of Colchicine in the Treatment of COVID-19 Patients: A Systematic Review and Meta-Analysis
Authors: Toro-Huamanchumo Carlos J. , Benites-Meza Jerry K. , Mamani-García Carlos S. , Bustamante-Paytan Diego , Gracia-Ramos Abraham Edgar , Diaz-Vélez Cristian , Barboza Joshuan J.
Published: May 2022

Objective: We assessed the efficacy of colchicine in COVID-19 patients through a systematic review. Methods: Six databases were searched until March 2022 for studies assessing colchicine versus control in hospitalized patients with COVID-19. The primary outcome was mortality, and secondary outcome was length of hospitalization. Inverse variance and random effect meta-analyses were performed. The strength of evidence was assessed using GRADE. Results: Nine studies (five randomized clinical trials (RCTs) and four non-randomized studies of intervention (NRSI); n = 13,478). Colchicine did not reduce mortality in comparison with the standard of care in RCTs (RR 0.99; 95%CI 0.90 to 1.10; p = 0.90); however, it did reduce mortality in NRSI studies (RR 0.45; 95%CI 0.26 to 0.77; p = 0.02). In the analysis of RCTs, colchicine did not reduce the length of hospitalization in comparison with the standard of care (MD: −2.25 days; 95%CI: −9.34 to 4.84; p = 0.15). Most studies were scored as having a high risk of bias. Quality of evidence was very low for primary and secondary outcomes. Conclusion: Colchicine did not reduce the mortality and length of hospitalization in comparison with the standard of care in hospitalized patients with COVID-19. The published evidence is insufficient and of very low quality to recommend treatment in patients with COVID-19.
The Use of Levosimendan after Out-of-Hospital Cardiac Arrest and Its Association with Outcome—An Observational Study
Authors: Rysz Susanne , Fagerlund Malin Jonsson , Lundberg Johan , Ringh Mattias , Hollenberg Jacob , Lindgren Marcus , Jonsson Martin , Djärv Therese , Nordberg Per
Published: May 2022

Background: Levosimendan improves resuscitation rates and cardiac performance in animal cardiac arrest models. The aim of this study was to describe the use of levosimendan in out-of-hospital cardiac arrest (OHCA) patients and its association with outcome. Methods: A retrospective observational study of OHCA patients admitted to six intensive care units in Stockholm, Sweden, between 2010 and 2016. Patients treated with levosimendan within 24 h from admission were compared with those not treated with levosimendan. Propensity score matching and multivariable logistic regression analysis were used to assess the association between levosimendan treatment and 30-day mortality Results: Levosimendan treatment was initiated in 94/940 (10%) patients within 24 h. The proportion of men (81%, vs. 67%, p = 0.007), initial shockable rhythm (66% vs. 37%, p < 0.001), acute myocardial infarction, AMI (47% vs. 24%, p < 0.001) and need for vasoactive support (98% vs. 61%, p < 0.001) were higher among patients treated with levosimendan. After adjustment for age, sex, bystander cardiopulmonary resuscitation, witnessed status, initial rhythm and AMI, the odds ratio (OR) for 30-day mortality in the levosimendan group compared to the no-levosimendan group was 0.94 (95% Confidence interval [CI], 0.56–1.57, p = 0.82). Similar results were seen when using a propensity score analysis comparing patients with circulatory shock. Conclusions: In this observational study of OHCA patients, levosimendan was used in a limited patient group, most often in those with initial shockable rhythms, acute myocardial infarction and with a high need for vasopressors. In this limited patient cohort, levosimendan treatment was not associated with 30-day mortality. However, a better matching of patient factors and indications for use is required to derive conclusions on associations with outcome.
The Rate of and Factors Associated with Delivery by Caesarean Section among Women with Epilepsy: Time Trend in a Single-Centre Cohort in Mazovia, Poland
Authors: Majkowska-Zwolińska Beata , Jędrzejczak Joanna
Published: May 2022

Data from literature suggest that the rate of caesarean section (CS) in women with epilepsy (WWE) is higher than in the general population. In Poland, there is neither a national registry nor another data set to access the outcome of pregnancy in WWE. Therefore, we address this gap by prospectively studying CS rates among 1021 WWE pregnancies at a single centre, their trends over time, and factors increasing the likelihood of the CS. To determine whether the diagnosis of epilepsy itself increased this likelihood, mixed models were used to analyse the contributions of specific variables, including the presence of seizures at different pregnancy-related timepoints. Over 20 years, the mean rate of CS in WWE was progressively growing and was higher than in the general population in Mazovia (47% vs. 32%). Generalized seizures in the third trimester increased the likelihood of CS with the highest odds (OR 4.4). The most frequent indication for a CS was obstetric (58.1%), followed by epilepsy-related (25.2%). Almost half of women who indicated epilepsy as the sole reason for CS had no seizure during pregnancy, and nearly 70% did not have generalized seizures. This suggests the overuse of epilepsy as an indication of CS and encourages defining more strict criteria.
Correlating Ocular Physiology and Visual Function with Mild Cognitive Loss in Senior Citizens in Taiwan
Authors: Su Kuo-Chen , Cheng Hong-Ming , Chu Yu , Lu Fang-Chun , Tsai Lung-Hui , Cheng Ching-Ying
Published: May 2022

Purpose: The transition of Taiwan from an aging to a super-aging society has come with a cost as more elderly now suffer from cognitive impairment. The main purpose of our study was to investigate if early detection can be developed so that timely intervention can be instituted. We analyzed the correlation of cognitive function with ocular physiology and visual functions between senior citizens aged 60 years or older in Taiwan. Methods: Thirty-six healthy subjects were recruited for the study. Addenbrooke’s cognitive examination III (ACE-III), binocular functions (including objective and subjective refraction, distance and near dissociated phoria, stereopsis, contrast sensitivity, adult developmental eye movement (ADEM), and ocular physiology (by using optical coherence tomography, OCT, and macular pigment measurement, MPS) were performed, and the data were analyzed via independent t-test, chi-square test, Pearson correlation, linear regression, and ROC (receiver operating characteristic) curve. Results: Data analysis showed that (1) patients with poor eye movement had a strong correlation with the total score and all dimensions of cognitive functions, (2) the thickness of the macula had a strong correlation with attention and memory, and (3) patients with poor eye movement and poor stereopsis in combination with thinner inferior macula appeared to have lower cognitive abilities. Discussion and Conclusions: Cognitive dysfunction is not readily identified during the early stage of cognitive decline. The use of simple and inexpensive ADEM or stereopsis test and comparing the OCT results that are popular in optometry clinics for reference can be diagnostic in identifying patients with mild cognitive impairments. With the combined use of macular pigment density or retinal thickness measurements, it was possible to effectively predict the early degradation of cognition.
OSDI Questions on Daily Life Activities Allow to Detect Subclinical Dry Eye in Young Contact Lens Users
Authors: Pastor-Zaplana José Ángel , Borrás Fernando , Gallar Juana , Acosta M. Carmen
Published: May 2022

Dry eye disease (DED) is difficult to detect in young contact lens (CL) wearers, who usually have no signs, mild symptoms and an ocular surface disease index (OSDI) below the DED diagnosis values (OSDI ≥ 13). We investigate if some of the 12 OSDI questions (OSDI A—ocular symptoms; OSDI B—vision-related functionality; OSDI C—environmental triggers) contribute the most to classify young CL as symptomatic. TBUT and tear volume are also measured. Age, gender and refraction error-matched eye glasses (EG) wearers participated as the control. CL and EG data were compared with t-test and z-test. Confusion matrices and logistic correlation analyses were performed to define the contribution of each OSDI question to classify symptomatic subjects. OSDI classified symptomatic CL better than the tear volume or TBUT values. In CL, only OSDI B and C values were significantly higher in symptomatic vs. asymptomatic subjects (p < 0.001), while values of all twelve OSDI questions were significantly higher in symptomatic vs. asymptomatic EG (p < 0.05–0.001). All OSDI questions contribute equally to identify symptomatic EG, while only OSDI B questions on daily life visual functions are significant to classify symptomatic CL wearers at risk to develop DED or at a subclinical stage. CL wearers scoring ≥ 2 on the OSDI B questions should be considered for preventive treatments, even if their clinical sings are scarce or absent.
Strategic Crisis Response of Shipping Industry in the Post COVID-19 Era: A Case of the Top 10 Shipping Lines
Authors: Sun Zhikuan , Zhang Yan
Published: May 2022

Shipping has played a pivotal role during the epidemic, ensuring that the global logistics functions without disruption. COVID-19 hit various industries around the world, and shipping was no exception. How the shipping industry responds to the crisis and simultaneously shoulders its respective responsibility in the world’s battling the crisis is thus worth exploring in depth. This study takes the top 10 global container shipping capacity liners, which account for 84.7% of the worldwide capacity, as research objects. A corpus for text analysis was constructed collecting press releases and advisories issued on official websites of these 10 container shipping companies from January 2020 to July 2021. Comparison studies were made horizontally among ten shipping companies and longitudinally for crisis evolution patterns into three sub-corpora of Pre/early-Crisis, Crisis-in-Progression, and Post-COVID-19-Era. Quantitative findings were explored and elaborated further under a comprehensive theoretical framework integrating crisis management and communication, corporate social responsibility (CSR), and maritime management (MM). The extracted positive and negative keywords revealed textual characteristics and emergency response strategies on the part of shipping lines in the Pre/early-Crisis, Crisis-in-Progression, and Post-COVID-19-Era. The inclusion of the themes of pursuing sustainability in the shipping lines’ responses to such worldwide crisis as COVID-19 is out of the common knowledge of crisis management but reveals the commitment and strategies on the part of the industry. The findings provide a reasonably comprehensive picture of the efforts made by large container shipping companies to respond to COVID-19 and the measures taken to soothe stakeholders. This paper extends and relearns crisis management, CSR, and MM theories through integrating the fulfilling of cooperate social responsibilities in maritime management as the cooperate crisis responses, thus proposing the integrity of the three topics. Moreover, management recommendations are provided for shipping company management, IMO, and port authorities.
Energy-Loss Straggling and Delta-Ray Escape in Solid-State Microdosimeters Used in Ion-Beam Therapy
Authors: Magrin Giulio , Barna Sandra , Meouchi Cynthia , Rosenfeld Anatoly , Palmans Hugo
Published: May 2022

Microdosimetry is increasingly adopted in the characterization of proton and carbon ion beams used in cancer therapy. Spectra and mean values of lineal energy calculated in frequency and dose are seen by many as the tools which, by complementing dosimetric measurements, allow for the most complete characterization of the therapeutic radiation fields. The urgency is now to consolidate the experience and converge to commonly accepted methodologies. In this context, the purpose of this work is to study the effects of the energy-loss straggling and the delta-ray escape, considering slab-sensitive volumes; these are, in fact, the typical shapes of solid-state microdosimeters, which are widely used in investigating light ion therapy beams. The method considers the energy distribution of delta rays resulting from the collision of the impinging ion and, taking into account the escape, convolutes it with itself as many times as the expected number of collisions in the sensitive volume thickness. The resulting distribution is compared to the experimental microdosimetric spectrum showing a substantially good agreement. The extension of the methodology to a wider range of ion energy and detector characteristics is instrumental for a detector-independent microdosimetric assessment of the radiation fields.
Hot Water Treatment Causes Lasting Alteration to the Grapevine (Vitis vinifera L.) Mycobiome and Reduces Pathogenic Species Causing Grapevine Trunk Diseases
Authors: Lade Sarah B. , Štraus Dora , Buñol Arnau , Oliva Jonàs
Published: May 2022

The effective management of grapevine trunk diseases (GTDs) is an ongoing challenge. Hot water treatment (HWT) is an environmentally friendly and economically viable option; however, the short-term effects of HWT on grapevine (Vitis vinifera L.) health and production are not fully understood. The aim of this study was to compare the effects of HWT on plant growth and fungal community structure in nursery stock until plants were completely established in the field. We assessed eleven graft and three rootstock varieties from four local nurseries in a region of Catalonia (NE Spain) where GTDs are a serious threat. After treatment, the plants were left to grow under field conditions for two growing seasons. Metabarcoding of the ITS region was used to study the mycobiomes of plant graft unions and root collars. We also assessed the influence of plant physiological indicators in community composition. Hot water treatment caused lasting changes in GTD communities in both the root collar and graft union that were not always characterized as a reduction of GTD-related fungi. However, HWT reduced the relative abundance of some serious GTD-associated pathogens such as Cadophora luteo-olivacea in graft tissues, and Phaeomoniella chlamydospora and Neofusicoccum parvum in the root collar. Treatment had the greatest influence on the total and GTD-related fungal communities of Chardonnay and Xarel·lo, respectively. Total community variation was driven by treatment and nursery in rootstocks, whereas HWT most significantly affected the GTD community composition in R-110 rootstock. In conclusion, changes in fungal abundance were species-specific and mostly dependent on the plant tissue type; however, HWT did reduce plant biomass accumulation in the short-term.
Low Inflammatory Stimulus Increases D2 Activity and Modulates Thyroid Hormone Metabolism during Myogenesis In Vitro
Authors: Oliveira Thamires Siqueira de , Shimabukuro Marilia Kimie , Monteiro Victoria Regina Siqueira , Andrade Cherley Borba Vieira , Boelen Anita , Wajner Simone Magagnin , Maia Ana Luiza , Ortiga-Carvalho Tania Maria , Bloise Flavia Fonseca
Published: May 2022

Thyroid hormone (TH) signaling controls muscle progenitor cells differentiation. However, inflammation can alter muscle TH signaling by modulating the expression of TH transporters (Slc16a2), receptors (Thra1), and deiodinase enzymes (Dio2 and Dio3). Thus, a proinflammatory environment could affect myogenesis. The role of a low-grade inflammatory milieu in TH signaling during myogenesis needs further investigation. Herein, we aimed to study the impact of the bacterial lipopolysaccharide (LPS)-induced inflammatory stimulus on the TH signaling during myogenesis. C2C12 myoblasts differentiation was induced without (CTR) or with 10 ng/mL LPS presence. The myoblasts under LPS stimulus release the proinflammatory cytokines (IL-6 and IL-1β) and chemokines (CCL2 and CXCL-1). LPS decreases Myod1 expression by 28% during the initial myogenesis, thus reducing the myogenic stimulus. At the same time, LPS reduced the expression of Dio2 by 41% but doubled the D2 enzymatic activity. The late differentiation was not affected by inflammatory milieu, which only increased the Slc16a2 gene expression by 38%. LPS altered the intracellular metabolism of TH and reduced the initial myogenic stimulus. However, it did not affect late differentiation. Increased intracellular TH activation may be the compensatory pathway involved in the recovery of myogenic differentiation under a low-grade inflammatory milieu.
Methodologies and MR Parameters in Quantitative Magnetic Resonance Neurography: A Scoping Review Protocol
Authors: Balsiger Fabian , Wagner Benedikt , Jende Johann M. E. , Marty Benjamin , Bendszus Martin , Scheidegger Olivier , Kurz Felix T.
Published: May 2022

Magnetic resonance neurography (MRN), the MR imaging of peripheral nerves, is clinically used for assessing and monitoring peripheral neuropathies based on qualitative, weighted MR imaging. Recently, quantitative MRN has been increasingly reported with various MR parameters as potential biomarkers. An evidence synthesis mapping the available methodologies and normative values of quantitative MRN of human peripheral nerves, independent of the anatomical location and type of neuropathy, is currently unavailable and would likely benefit this young field of research. Therefore, the proposed scoping review will include peer-reviewed literature describing methodologies and normative values of quantitative MRN of human peripheral nerves. The literature search will include the databases MEDLINE (PubMed), EMBASE (Ovid), Web of Science, and Scopus. At least two independent reviewers will screen the titles and abstracts against the inclusion criteria. Potential studies will then be screened in full against the inclusion criteria by two or more independent reviewers. From all eligible studies, data will be extracted by two or more independent reviewers and presented in a diagrammatic or tabular form, separated by MR parameter and accompanied by a narrative summary. The reporting will follow the guidelines of the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR). Upon completion, the scoping review will provide a map of the available literature, identify possible gaps, and inform on possible future research. SCOPING REVIEW REGISTRATION: Open Science Framework 9P3ZM.
A Study on the Role of Pre-Cleaning and a New Method to Strengthen Gate Oxide Quality
Authors: Lee In-Kyum , Choi Byoung-Deog
Published: May 2022

Isopropyl alcohol (IPA) has been conventionally used for pre-cleaning processes. As the device size decreased, the gate oxide layer became thinner. As a result, the quality of the gate oxide was degraded by a pre-cleaning process, and oxide reliabilities and product yield were affected. In this study, we investigate whether the carbon generated on the silicon interface after the IPA drying process might have induced gate oxide breakdown. Time-dependent dielectric breakdown (TDDB) failure increased in frequency since carbon contaminations were increased in the oxide according to the amount of IPA. Organic contaminations resulted in a lower energy level, and electron tunneling occurred through the gate oxide. When an external electric field was applied, organic materials in the gate oxide layer were aligned, and a percolation path formed to cause breakdown. Finally, we suggest a new cleaning method using carbon-free O3 deionized (DI) water as a dry-cleaning method to improve oxide dielectric breakdown. An O3 DI dry cleaning process could reduce carbon particles in the oxide layer and decrease gate oxide failure by 7%.