bluuebunny/crossref_metadata_2025 huggingface dataset:
Predictors of Hospitalization for Patients Presenting to Emergency Department with COVID-19 Infection
Authors: Alsagban Alhareth , Singh Amteshwar , Baidya Anurima , Dalal Monika , Khaliq Waseem
Published: January 2025

Background: Predictors of morbidity and mortality in hospitalized COVID-19 patients have been extensively studied. However, comparative analyses of predictors for hospitalization versus discharge from the emergency department remain limited. Methods: This retrospective study evaluated predictors of hospitalization among adults (≥18 years) presenting to the emergency department with COVID-19 infection between 1 March 2020 and 15 June 2020. Data were obtained from electronic health records across five hospitals within the Johns Hopkins Health System, encompassing 2513 beds. Multivariable logistic regression models were employed to assess the association between sociodemographic characteristics, clinical symptoms, and comorbidities with hospitalization. Results: Of the 2767 patients presenting to the emergency department, 1678 (61%) were hospitalized, while 1089 (39%) were discharged from the emergency department. Hospitalized patients were older (mean age 61.8 years, SD 18), more likely to be African American and White, non-Hispanic, unemployed or on disability, medically insured, had access to primary care, and presented on weekends. Smoking status, alcohol use, and higher comorbidity burden (mean age-adjusted Charlson Comorbidity Index > 3) were also more prevalent with hospitalization. Dyspnea was a prominent clinical feature among hospitalized patients. After adjusting for sociodemographic and clinical risk factors, significant predictors of hospitalization included health insurance (OR 3.44; 95% CI: 1.98–6), having a primary care (OR 1.85; 95% CI: 1.33–2.59), presentation from a non-home locale (OR 4.04; 95% CI: 1.93–8.47), age-adjusted CCI > 3 (OR 1.72; 95% CI: 1.11–2.68), dyspnea (OR 2.22; 95% CI: 1.56–3.17), neutrophil-to-lymphocyte ratio ≥ 3 (OR 2.17; 95% CI: 1.54–3.06), and an abnormal chest radiograph findings (OR 6.17; 95% CI: 4.40–8.66). Interestingly, obesity, defined as a BMI ≥ 30 kg/m2 (OR 0.45; 95% CI: 0.32–0.64), and the presence of fever (OR 0.64; 95% CI: 0.43–0.95) were found to be associated with a decreased likelihood of hospitalization. Conclusions: Future studies are warranted to further explore predictors of COVID-19 hospitalization, with particular focus on the implications of weekend presentations and the paradoxical relationship of obesity with COVID-19 health outcomes. These findings could inform the development of triage models to enhance preparedness for future pandemics.
Diagnostic Utility of Procalcitonin and Lactate Determination in Cerebrospinal Fluid for the Diagnosis of Neonatal Meningitis
Authors: Borowiak Anna , Safranow Krzysztof , Sarna Angela , Łoniewska Beata
Published: January 2025

Objectives: The diagnosis of meningitis is based on microbiological analysis of the cerebrospinal fluid, and the evaluation of cytosis and biochemical parameters such as protein and glucose levels. Sometimes when there is a traumatic lumber puncture, the cerebrospinal fluid is bloody, which makes it difficult to diagnose. The objective of the study was to examine the performance of cerebrospinal fluid (CSF) procalcitonin (PCT) and lactate as potential markers for the diagnosis of meningitis in neonates. Methods: 110 neonates who qualified for lumbar puncture were enrolled in the study. On the basis of CSF analysis, the neonates were classified into two groups: the meningitis group (n = 33) and the non-meningitis group (n = 77). PCT and lactate in CSF and established CSF parameters were recorded. Results: Median CSF PCT level was significantly higher in the meningitis group compared to non-meningitis: 0.93 (0.39–1.59) vs. 0.34 (0.195–0.74) ng/mL, p < 0.000001. Median CSF lactate level was significantly higher in the meningitis group compared to non-meningitis: 3.1 (2.27–3.96) vs. 1.78 (1.38–3.19) mmol/L, p < 0.001. At a cutoff of 0.35 ng/mL, CSF PCT had a sensitivity of 82% and specificity of 52% in the diagnosis of meningitis (AUC = 0.7). At a cutoff of 2.07 mmol/L, CSF lactate had a sensitivity of 84% and specificity of 60% in the diagnosis of meningitis (AUC = 0.701). Conclusions: Concentrations in CSF of PCT and lactate in neonates with meningitis are significantly higher than in the non-meningitis group. None of the biochemical indicators studied met the criteria for a marker for the diagnosis of meningitis as a single indicator.
Comparing Repaired Subscapularis Tendon Integrity Using Ultrasound in Onlay Versus Inlay Reverse Shoulder Arthroplasty
Authors: Kapilan Shri , Nabergoj Marko , Lädermann Alexandre , Collin Philippe
Published: January 2025

Background: The importance of the subscapularis tendon in reverse shoulder arthroplasty (RSA) has been increasingly emphasized lately. Recent studies have indicated that a repaired subscapularis tendon has better functional outcomes. This study is aimed at comparing the healing rate of repaired subscapularis tendons between onlay and inlay Bony Increased Offset-Reversed Shoulder Arthroplasty (BIO-RSA). Methods: This retrospective comparative review covers all patients who underwent BIO-RSA at a single center, comprising 189 cases performed by on a single surgeon from January 2012 till December 2021. We included all patients who underwent subscapularis tenotomy repair and who had a tendon ultrasound (US) examination at six months postoperatively (as requested in this single surgeon’s usual protocol). These patients were divided into two comparable groups, an onlay group and an inlay group. Healing status was determined using the Sugaya classification, with healed subscapularis tendons defined as having Sugaya type I–III integrity and the unhealed tendons as having Sugaya type IV and V integrity. Results: In total, 2 patients were excluded because ultrasound was not performed (they missed their appointment); 187 patients were evaluated; 98 patients underwent an onlay BIO-RSA; and 89 patients underwent an inlay BIO-RSA. The healing rate of the repaired subscapularis tendon was 73% in the onlay group and 56% in the inlay one (p = 0.020). Conclusions: The onlay systems may enhance subscapularis tendon healing compared to the inlay one, possibly due to the preserved intramedullary vascularity and the near-normal tendon excursion that can be achieved by the onlay system. Choosing an onlay design can minimize bone cuts during RSA, while achieving good subscapularis tendon healing.
Supra-Normal Ejection Fraction at Hospital Admission Stratifies Mortality Risk in HFpEF Patients Aged ≥ 70 Years
Authors: Sonaglioni Andrea , Lonati Chiara , Scime’ Valentina , Nicolosi Gian Luigi , Bruno Antonino , Lombardo Michele , Harari Sergio
Published: January 2025

Background: During the last few years, significant pathophysiological differences between heart failure (HF) patients with “normal” ejection fraction (EF) (50% to 64%) and those with supra-normal EF (≥65%) have been highlighted. However, these distinct EF phenotypes have been poorly investigated in elderly patients aged ≥70 y. Accordingly, the present study aimed at assessing the clinical and echocardiographic characteristics of a retrospective cohort of elderly HFpEF patients (aged ≥ 70 y), categorized on the basis of “normal” EF (50 to 64%) or “supra-normal” EF (≥65%). Methods: All patients aged ≥ 70 y that were discharged from our Institution with a first diagnosis of HF with preserved EF (HFpEF) between January 2020 and March 2021 entered the study. All patients underwent clinical evaluation, blood tests, and transthoracic echocardiography. The primary endpoint was “all-cause mortality”, while the secondary one was the composite “all-cause mortality or rehospitalization for all causes” over a mid-term follow-up. Results: A total of 200 HFpEF patients (86.4 ± 6.6 y, 70% females) were retrospectively evaluated. The “normal” EF group (n = 99) and the “supra-normal” EF one (n = 101) were separately analyzed. Compared to patients with “normal” EF, those with “supra-normal” EF were older, with greater comorbidity burden, and moderate-to-severe frailty status. The mean follow-up duration was 3.6 ± 0.3 y. During follow-up, 79 patients died, and 73 were rehospitalized. In the multivariate Cox regression analysis, age (HR 1.09, 95% CI 1.03–1.16, p = 0.002), EF (HR 1.08, 95% CI 1.03–1.14, p = 0.004), tricuspid annular plane systolic excursion (TAPSE)/systolic pulmonary artery pressure (sPAP) ratio (HR 0.14, 95% CI 0.03–0.61, p = 0.009), and infectious disease occurring during the baseline stay (HR 7.23, 95% CI 2.41–21.6, p < 0.001) were independently associated with the primary endpoint in the whole study population. EF (HR 1.04, 95% CI 1.01–1.07, p = 0.02) also predicted the secondary endpoint. EF ≥65% was the best cut-off to predict both endpoints. Conclusions: “Supra-normal” EF (≥65%) at hospital admission is independently associated with all-cause mortality and rehospitalization for all causes in elderly HFpEF patients over a mid-term follow-up.
Assessing the TNM Classification for Periprosthetic Joint Infections of the Knee: Predictive Validity for Functional and Subjective Outcomes
Authors: Kienzle Arne , Walter Sandy , Köhli Paul , Gwinner Clemens , Hardt Sebastian , Müller Michael , Perka Carsten , Donner Stefanie
Published: January 2025

Background: Periprosthetic joint infection (PJI) following knee arthroplasty can significantly compromise patient mobility and quality of life. The newly proposed TNM classification system, adapted from oncology, categorizes PJI severity but has not yet been correlated with both subjective and objective outcomes post PJI treatment. Objective: This study evaluates the applicability of the TNM classification system for predicting outcomes in knee PJI revision surgeries. Methods: We conducted a retrospective analysis of 108 patients who underwent revision surgeries for knee PJI at our institution from January 2012 to January 2023. We assessed the correlation between the TNM classification and postoperative outcomes using the Knee Society Score (KSS) function and knee score, as well as the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC). Results: The TNM classification demonstrated that higher ‘T’ stages were significantly associated with worse functional and subjective outcomes. The ‘N’ classification had limited predictive value, likely due to treatment adjustments based on pathogen type. The ‘M’ classification correlated with functional outcomes but not with subjective scores, suggesting that patients with more severe preoperative comorbidities might adjust their expectations. Conclusions: While the TNM classification shows potential, its current form as a prognostic tool in PJI management is limited. Enhancing the ‘T’ component, coupled with the integration of a validated morbidity score such as the CCI could improve its prognostic value. Despite its shortcomings, the TNM system may still provide valuable prognostic insights for both patients and surgeons in tackling complex PJI.
Health Professionals’ Preferences for Next-Generation Sequencing in the Diagnosis of Suspected Genetic Disorders in the Paediatric Population
Authors: Nurchis Mario , Altamura Gerardo , Raspolini Gian , Capobianco Enrico , Salmasi Luca , Damiani Gianfranco
Published: January 2025

Background/Objectives: Next-generation sequencing (NGS) can explain how genetics influence morbidity and mortality in children. However, it is unclear whether health providers will perceive and use such treatments. We conducted a discrete choice experiment (DCE) to understand Italian health professionals’ preferences for NGS to improve the diagnosis of paediatric genetic diseases. Methods: The DCE was administered online to 125 health professionals in Italy. We documented attributes influencing professionals’ decisions of NGS, including higher diagnostic yield, shorter counselling periods, cost, turnaround time, and the identification of fewer variants of unknown significance. Results: Results show that factors such as higher diagnostic yield, shorter counselling periods, lower costs, and faster turnaround times positively influenced the adoption of NGS tests. Willingness to pay (WTP) estimates varied from EUR 387 (95% CI, 271.8–502.9) for 7% increase in the diagnostic yield to EUR 469 (95% CI, 287.2–744.9) for a decrease of one week in the turnaround time. Responders would reduce diagnostic yield by 7% to decrease the turnaround time by one week in both the preference and the willingness to trade (WTT) spaces. Respondents prioritised diagnostic yield (RI = 50.36%; 95% CI 40.2–67.2%) compared to other attributes. Conclusions: therefore, health professionals value NGS for allowing earlier, more accurate genetic diagnoses.
Incentivizing Video-on-Demand Subscription Intention Through Tiered Discounts and Anti-Piracy Messages
Authors: Redondo Ignacio , Serrano Diana
Published: January 2025

Subscription video-on-demand (SVOD) platforms face high churn rates and substantial revenue losses from SVOD content piracy, all of which limit their ability to invest in acquiring/creating content compelling enough to win and retain subscribers. Based on social exchange theory, this study argues that platforms can improve relationships with SVOD content users by offering tiered discounts in exchange for advertising/loyalty and by promoting anti-piracy messages with a prosocial (threatening) approach that emphasizes harm to filmmakers (punishment for pirates). We hypothesize that these incentives enhance subscription intention when the incentive specifications (advertising levels, loyalty levels, message approach, and message credibility) match the public’s heterogeneous dispositions (advertising attitude, loyalty attitude, justice sensitivity, and fear of punishment). In a survey on the intention to subscribe to a hypothetical new platform, we confirmed the hypothesized interactions for advertising-based discounts, loyalty-based discounts, and prosocial messages, but did not find support for threatening messages. Further exploration showed that the evaluation of platform content was much more influential than any other incentive and that tiered loyalty discounts had a remarkable capacity to enhance subscription intention. This study’s findings may help shape incentives that are more satisfying to users and ultimately more profitable for platforms.
The Analysis of Spatiotemporal Changes in Vegetation Coverage and Driving Factors in the Historically Affected Manganese Mining Areas of Yongzhou City, Hunan Province
Authors: Liu Jinbin , He Zexin , Shi Huading , Zhao Yun , Wang Junke , Liu Anfu , Li Li , Zhu Ruifeng
Published: January 2025

Manganese ore, as an important strategic metal resource for the country, was subject to unreasonable mining practices and outdated smelting technologies in early China, leading to severe ecological damage in mining areas. This study examines the trends in vegetation cover change in the historical manganese mining areas of Yongzhou under the influence of policy, providing technical references for mitigating the ecological impact of these legacy mining areas and offering a basis for adjusting mine restoration policies. This paper takes the manganese mining area in Yongzhou City, Hunan Province as a case study and selects multiple periods of Landsat satellite images from 2000 to 2023. By calculating the Normalized Difference Vegetation Index (NDVI) and the Fractional Vegetation Coverage (FVC), the spatiotemporal changes and driving factors of vegetation coverage in the Yongzhou manganese mining area from 2000 to 2023 were analyzed. The analysis results show that, in terms of time, from 2000 to 2012, the vegetation coverage in the manganese mining area decreased from 0.58 to 0.21, while from 2013 to 2023, it gradually recovered from 0.21 to 0.40. From a spatial perspective, in areas where artificial reclamation was conducted, the vegetation was mainly mildly and moderately degraded, while in areas where no artificial restoration was carried out, significant vegetation degradation was observed. Mining activities were the primary anthropogenic driving force behind the decrease in vegetation coverage, while effective ecological protection projects and proactive policy guidance were the main anthropogenic driving forces behind the increase in vegetation coverage in the mining area.
Glass/Polyester Laminates Modified with L-Arginine Phosphate—Effects on the Flammability and Smoke Emission
Authors: Dowbysz Adriana , Samsonowicz Mariola , Kukfisz Bożena , Koperniak Piotr
Published: January 2025

Flammability and smoke generation of glass-fiber-reinforced polyester laminates (GFRPs) modified with L-arginine phosphate (ArgPA) have been investigated. The composition, structure, and thermal degradation processes of ArgPA were assessed by the elemental, FTIR, and thermogravimetric analyses. Flammability and smoke emission of GFRPs varying by different amounts (5–15 wt.%) of bio-based flame retardant (FR) prepared via hand lay-up method were assessed in terms of the limiting oxygen index (LOI) and smoke density tests. It was observed that the addition of ArgPA results in the formation of a charred layer with visible bubbles. The LOI of GFRP with 15 wt.% of ArgPA increased from 20.73 V/V % (non-modified GFRP) to 24.55 V/V %, and the material classification was improved from combustible to self-extinguishing. FRs usually increase the specific optical density of smoke, which was also observed for ArgPA-modified GFRPs. However, the specific optical density of smoke at the 4th minute of measurement (Ds(4)) obtained for ArgPA-modified GFRPs was lower than for GFRPs modified with commercially used APP. TG/FTIR studies of resin modified with ArgPA revealed the presence of phosphorus compounds and non-combustible gases in the decomposition products. Results demonstrate the potential of ArgPA as an effective, bio-based FR for the enhancement of GFRP fire safety.
Stripe Noise Removal in Blazed Grating Generation for Electrically Tunable Beam Deflector
Authors: Kim Woosup , Kim Taeyoung , Do Jun , Ma Heechang , Yoon Heesun , Won Kanghee
Published: January 2025

In this paper, we demonstrate a blazed phase grating to achieve tunable beam steering and propose a novel algorithm to reduce the stripe noise in wrapped phase. To control the diffraction angle to steer light to the desired direction, an electrically tunable transmission-type beam deflector based on liquid crystals is introduced, and electric fields are applied to the patterned indium tin oxide electrodes to change its phase retardation. Two different 2π phase-wrapping methods are applied to obtain various diffraction angles within the minimum cell-gap, and the method of equal interval of phase achieves a worthwhile diffraction efficiency compared to the methods based on equal interval of diffraction angle. The proposed method is able to completely eliminate the stripe noise in all steering angles that helps to improve the diffraction efficiency.
A Critical Review of the Decarbonisation Potential in the U.K. Cement Industry
Authors: Sherif Ziyad , Sarfraz Shoaib , Jolly Mark , Salonitis Konstantinos
Published: January 2025

As urbanisation and infrastructure development continue to drive rising cement demand, the imperative to significantly reduce emissions from this emissions-intensive sector has become increasingly urgent, especially in the context of global climate goals such as achieving net zero emissions by 2050. This review examines the status, challenges and prospects of low-carbon cement technologies and mitigation strategies through the lens of the U.K. cement industry. A mixed-methods approach was employed, combining structured literature searches across academic databases with analyses of industry reports, market data and technological roadmaps to ensure a comprehensive evaluation. Following an outline of cement production, resource flows and the sector’s landscape in the U.K., the review delves into an array of decarbonisation pathways. This includes deploying the best available technologies (BATs), fuel switching, carbon capture utilisation and storage (CCUS), clinker substitution and low-carbon cement formulations. A critical assessment is provided on the technological readiness, costs, resource availability considerations and scalability aspects governing the widespread implementation prospects of these approaches within the U.K. cement industry. Furthermore, this study proposes a roadmap that considers priority avenues and policy needs essential for facilitating the transition towards sustainable cement production aligned with the U.K.’s net zero obligations by 2050. This evaluation contributes significantly to the ongoing decarbonisation discourse by holistically mapping technological solutions and strategic imperatives tailored to the unique challenges and opportunities presented by the U.K. cement sector.
Microstructure, Hardness, and Wear Behavior of Layers Obtained by Electric Arc Hardfacing Processes
Authors: Balos Sebastian , Zlatanović Danka Labus , Janjatović Petar , Pećanac Milan , Erić Cekić Olivera , Rosić Milena , Stopić Srećko
Published: January 2025

Hardfacing is a welding-related technique aimed at depositing a harder and tougher layer onto a softer, less wear-resistant substrate or base metal. This process enhances the abrasion resistance of the component, increasing its durability under working conditions. A key feature of hardfacing is dilution, which refers to the mixing of the hardfacing layer and the base metal. In this study, shielded metal arc welding (SMAW) was employed to hardface structural steel using chromium carbide vanadium consumables, with results compared to AISI D2 cold-work tool steel. Four different SMAW parameters were tested, and the abrasive test was conducted against SiC discs. Wear rate, represented by the wear loss rate, was correlated to microstructure, scanning electron microscopy, energy-dispersive X-ray spectroscopy, hardness, microhardness, and surface roughness. The results showed that key SMAW parameters, such as welding speed and current, significantly influence wear resistance. Specifically, slower welding speeds and higher currents, which result in greater heat input, led to the increased wear resistance of the deposited layer through the mechanism of the inoculation of larger and harder carbides.
Software-Defined Value Stream Process Systems
Authors: Goebels Max , Schulte Alexander , Georgi Patrick , Heimberger Fabian , Schwalm Jannik , Liewerenz Oliver , Schäfer Adrian , Fleischer Jürgen , Verl Alexander , Möhring Hans-Christian , Schulze Volker , Matthiesen Sven , Parspour Nejila
Published: January 2025

The increasing volatility of the markets and the growing demand for customized products are challenges for future production to ensure maximum flexibility and adaptability. This paper introduces software-defined value stream process systems (SVPSs), a novel approach that extends the concept of software-defined manufacturing into autonomous, reconfigurable production systems. SVPSs establish a cyber–physical chain that links product features to requirements, enabling their fulfillment through modular machine and process hardware. A modular construction kit of individually combinable hardware and associated software modules is presented. These modules are coordinated via a digital process chain that enables holistic simulations, optimizations, and planning based on a Digital Twin. This system is based on software-defined manufacturing but extends it into autonomous reconfigurable machines. By enabling virtual planning and commissioning of entire production lines, the SVPS concept provides an efficient and adaptable solution to meet the demands of volatile markets.
Is the Beck Depression Inventory (BDI) a Useful Tool for Predicting IVF Success?
Authors: Šljivancanin Dragiša , Vidaković Snežana , Kisić Tepavčević Darija , Petrović Bojana , Šljivančanin Una , Dokić Milan
Published: January 2025

Background and Objectives: Infertility is a global problem. The interaction between depression and infertility seems bidirectional, and depression may negatively influence IVF outcomes. The Beck Depression Inventory (BDI) is one of the most extensively used instruments for diagnosing depression. The aim of this study was to assess the dynamics of depression in patients undergoing IVF utilizing the BDI. Materials and Methods: This prospective cohort study was conducted at the Clinic for Gynecology and Obstetrics of the University Clinical Center of Serbia in Belgrade, Serbia during the year 2019. Both partners in the IVF program were required to complete the paper-based BDI 5 to 10 days before starting ovarian stimulation, within the 48 h preceding oocyte aspiration and four weeks after embryo transfer. We sought to identify potential predictors of a favorable IVF outcome by using logistic regression modeling. Results: Our study enrolled 86 couples. The overall IVF efficacy in our cohort was 18.6%. A statistically significant logistic regression model (p = 0.001) managed to explain 47.6% of the variability. Increasing patient age, the presence of depression (BDI > 16) and the number of failed IVF cycles were found to be significant negative predictors of ongoing IVF success. Conclusions: Depression is more common in patients undergoing IVF than in the general population. Depression might increase the odds of IVF failure, lending credence to the idea that depression screening using BDI should be a routine part of the IVF process.
Technical Report on the New Ultrasound Lateral Mid-Shaft Approach to the Sciatic Nerve: A Never-Ending Story
Authors: Sepolvere Giuseppe , Tedesco Mario , Cibelli Mario , Cirillo Dario , Sparaco Angelo , Gagliardi Giuseppe , Costagliola Giuseppina , Cristiano Loredana , Scialdone Valeria Rita , Pasquariello Maria Rosaria , Di Zazzo Fabrizio , Merola Luigi , Della Valle Mirco , Arminio Daniela , Bottazzo Leonardo Maria , Folliero Marco , Ranieri Giorgio , Santonastaso Domenico Pietro , Coviello Antonio
Published: January 2025

The anatomy of the sciatic nerve allows it to be blocked at different levels using various anesthetic approaches. However, for several reasons, performing these approaches may be challenging or disadvantageous in specific categories of patients, particularly in obese patients. The objective of this brief technical report is to describe a new technical approach to sciatic nerve block, designed to simplify the procedure for certain categories of patients and less experienced practitioners. Since 2010, more than 5000 procedures have been performed by both experienced anesthesiologists and novice trainees in several hospitals. The ultrasound lateral mid-shaft technique appears to be a safe and effective method for performing a sciatic nerve block, even in obese patients with significant subcutaneous fat and unclear ultrasound images. This approach is particularly beneficial given the various anatomical variations that can occur. By targeting the mid-thigh area, the ultrasound beam accesses anatomical structures that are more superficial, improving the technique’s efficacy. Various hospital groups have been performing this technique as a routine procedure, achieving a success rate of nearly 100%. This impressive success rate exceeds that of other conventional techniques documented in the literature. Additionally, there have been significant improvements in comfort and ease for anesthetists. This method allows the anesthetic to spread around the paraneural sheath, covering the posterior femoral cutaneous nerve. Finally, it is performed in the supine position without the need to mobilize the lower limbs, ensuring patient comfort, especially in cases of fractures or lower limb injuries. Further studies are needed to confirm these results.
Combining In Vitro, In Vivo, and In Silico Approaches to Explore the Effect of Ceratonia siliqua and Ocimum basilicum Rich Phenolic Formula on Lipid Metabolism and Plasma Lipoprotein Oxidation in Mice Fed a High-Fat Diet: A Follow-Up Study
Authors: Moumou Mohammadine , Tayebi Amani , Hadini Abderrahmane , Noman Omar M. , Alhalmi Abdulsalam , Ahmoda Hamza , Amrani Souliman , Harnafi Hicham
Published: January 2025

Background/Objectives: Hyperlipidemia is a serious risk factor for cardiovascular diseases and liver steatosis. In this work, we explored the effect of an herbal formula (CBF) containing immature Ceratonia siliqua pods and Ocimum basilicum extracts on lipid metabolism disorders and lipoprotein-rich plasma (LRP) oxidation in mice. Methods: The phenolic composition was determined using HPLC-DAD analysis. The antioxidant activity was studied using various in vitro methods. Acute toxicity was evaluated in mice. Importantly, the effect of the CBF on lipid metabolism disorders was investigated in a high-fat diet (HFD) hyperlipidemia mouse model. An in silico study was carried out to predict underlying mechanisms. Results: The HPLC analysis revealed gallic acid, cinnamic acid, and naringenin as major phenolics of the carob pod aqueous extract. Concerning the basil hydro-ethanolic extract, rosmarinic, chicoric, caftaric, and caffeic acids were the main phenolics. Accordingly, the CBF prevented LRP oxidation in a concentration-dependent manner. This formula is not toxic in mice (LD50 > 2000 mg/kg body weight). Moreover, animals administered the CBF at 200 mg/kg/day presented a significant decline in their body weight gain, adipose tissue weight, plasma total cholesterol, low-density lipoprotein cholesterol (LDL-C) level, and glycaemia after 10 weeks’ treatment. Accordingly, the CBF decreased the plasma atherogenic index and the LDL-C to HDL-C ratio and reduced the level of fats accumulated in the liver. The molecular docking study revealed that chicoric, rosmarinic, and caftaric acids, and naringenin bound particularly strongly to many proteins involved in the regulation of lipid and cholesterol metabolism. This includes the HMG-CoA reductase, PPARα/γ, PCSK9, Cyp7a1, and ATP-citrate lyase. Conclusions: The CBF could be a good source of natural supplements, functional foods, and pharmaceuticals effective in managing hyperlipidemia and oxidative stress and preventing their related cardiovascular disorders.
Volatile Organic Metabolites as Potential Biomarkers for Genitourinary Cancers: Review of the Applications and Detection Methods
Authors: Holbrook Kiana L. , Lee Wen-Yee
Published: January 2025

Cancer is one of the leading causes of death globally, and is ranked second in the United States. Early detection is crucial for more effective treatment and a higher chance of survival rates, reducing burdens on individuals and societies. Genitourinary cancers, in particular, face significant challenges in early detection. Finding new and cost-effective diagnostic methods is of clinical need. Metabolomic-based approaches, notably volatile organic compound (VOC) analysis, have shown promise in detecting cancer. VOCs are small organic metabolites involved in biological processes and disease development. They can be detected in urine, breath, and blood samples, making them potential candidates for sensitive and non-invasive alternatives for early cancer detection. However, developing robust VOC detection methods remains a hurdle. This review outlines the current landscape of major genitourinary cancers (kidney, prostate, bladder, and testicular), including epidemiology, risk factors, and current diagnostic tools. Furthermore, it explores the applications of using VOCs as cancer biomarkers, various analytical techniques, and comparisons of extraction and detection methods across different biospecimens. The potential use of VOCs in detection, monitoring disease progression, and treatment responses in the field of genitourinary oncology is examined.
Comparison of Microfluidic Synthesis of Silver Nanoparticles in Flow and Drop Reactors at Low Dean Numbers
Authors: Nathanael Konstantia , Kovalchuk Nina M. , Simmons Mark J. H.
Published: January 2025

This study evaluates the performance of continuous flow and drop-based microfluidic devices for the synthesis of silver nanoparticles (AgNPs) under identical hydrodynamic and chemical conditions. Flows at low values of Dean number (De < 1) were investigated, where the contribution of the vortices forming inside the drop to the additional mixing inside the reactor should be most noticeable. In the drop-based microfluidic device, discrete aqueous drops serving as reactors were generated by flow focusing using silicone oil as the continuous phase. Aqueous solutions of reagents were supplied through two different channels merging just before the drops were formed. In the continuous flow device, the reagents merged at a Tee junction, and the reaction was carried out in the outlet tube. Although continuous flow systems may face challenges such as particle concentration reduction due to deposition on the channel wall or fouling, they are often more practical for research due to their operational simplicity, primarily through the elimination of the need to separate the aqueous nanoparticle dispersion from the oil phase. The results demonstrate that both microfluidic approaches produced AgNPs of similar sizes when the hydrodynamic conditions defined by the values of De and the residence time within the reactor were similar.
Inhibition of Salmonella enterica and Enterohemorrhagic Escherichia coli by Ethanolic Extracts of Pomegranate Peels
Authors: Wu Weifan , Mis Solval Kevin , Chen Jinru
Published: January 2025

This study compared the effect of phenolics extracted from four different types of pomegranate peels for controlling the growth of Salmonella enterica and enterohemorrhagic Escherichia coli. Cells of the two bacterial cultures (5 log CFU/mL) were treated in tryptic soy broth containing 0, 1, or 2% ethanolic extracts of four pomegranate peels for 5, 10, or 24 h at 25 °C. The surviving cells were recovered on a general-purpose agar and a selective medium. The extracts of three products were more effective (p ≤ 0.05) against E. coli than the extract of the remaining product, which had a similar activity to the extracts of two of the products used against S. enterica. Longer treatment time and higher extract concentration resulted in greater pathogen population reductions. The bacterial strains used had varied susceptibility to the extracts. Reductions in cell population positively correlated with the total contents of hydrolyzable tannins in the treatment systems. These findings suggested that ethanolic extracts of evaluated pomegranate peels had inhibitory activities against the two bacterial pathogens. This highlights the potential of pomegranate peels as a promising natural alternative to conventional antimicrobials for controlling foodborne pathogens like S. enterica and E. coli.
Extraction, Purification, Structural Characteristics, Biological Activities, and Applications of Polysaccharides from Gastrodia elata: A Review
Authors: Yang Yan , Hai Yonglin , Yang Yongcheng , Wen Rouyuan , Wang Le , Qian Yan , Zhang Jiaxian , Li Yongguo , Shi Zhilong , Zhang Haizhu
Published: January 2025

Gastrodia elata Blume is a well-known medicinal and edible plant in China, celebrated for its extensive history in traditional medicine and functional food applications. Among its key bioactive components, polysaccharides have drawn significant attention from researchers in the fields of health food and medicine due to their potential health benefits. Recent studies have revealed various biological activities associated with G. elata polysaccharides, including antioxidant, anti-tumor, anti-inflammatory, antibacterial, anti-aging, immune regulation, and neuroprotective properties. However, a comprehensive overview of these polysaccharides remains elusive. Specifically, relationship between the structure and activity of G. elata polysaccharides, along with the mechanisms through which various types exert their biological effects, has yet to be fully elucidated. This knowledge gap may impede the further development and utilization of G. elata polysaccharides in medicine, health products, food, and cosmetics. This paper provides a comprehensive overview of recent advancements in extraction, separation, purification, biological activities, and applications of G. elata polysaccharides. Additionally, it delves into structure-activity relationships and pharmacological mechanisms of these polysaccharides, giving support for future research to enhance their application in medicine, food, health products, and cosmetics.
Non-Stoichiometric BaxMn0.7Cu0.3O3 Perovskites as Catalysts for CO Oxidation: Optimizing the Ba Content
Authors: Díaz-Verde Álvaro , dos Santos Veiga Emerson Luiz , Beltrán-Mir Héctor , Illán-Gómez María José , Cordoncillo-Cordoncillo Eloísa
Published: January 2025

In this work, a series of BaxMn0.7Cu0.3O3 samples (x: 1, 0.9, 0.8, and 0.7, BxMC) was synthesized, characterized, and used as catalysts for CO oxidation reaction. All formulations were active for CO oxidation in the tested conditions. A correlation between the electrical conductivity, obtained by impedance spectroscopy, and the reducibility of the samples, obtained by H2-TPR, was observed. The Ba0.8Mn0.7Cu0.3O3 composition (B0.8MC) showed the best catalytic performance (comparable to that of the 1% Pt/Al2O3 reference sample) during tests conducted under conditions similar to those found in the exhaust gases of current gasoline engines. The characterization data suggest the simultaneous presence of a high Mn(IV)/Mn(III) surface ratio, oxygen vacancies, and reduced copper species, these two latter being key properties for ensuring a high CO conversion percentage as both are active sites for CO oxidation. The reaction temperature and the reactant atmosphere composition seem to be the most important factors for achieving a good catalytic performance, as they strongly determine the location and stability of the reduced copper species.
Effect of a Novel Food Rich in Miraculin on the Intestinal Microbiome of Malnourished Patients with Cancer and Dysgeusia
Authors: Plaza-Diaz Julio , Brandimonte-Hernández Marco , López-Plaza Bricia , Ruiz-Ojeda Francisco Javier , Álvarez-Mercado Ana Isabel , Arcos-Castellanos Lucía , Feliú-Batlle Jaime , Hummel Thomas , Palma-Milla Samara , Gil Angel
Published: January 2025

Background/Objectives: Dysgeusia contributes to malnutrition and worsens the quality of life of patients with cancer. Despite the different strategies, there is no effective treatment for patients suffering from taste disorders provided by the pharmaceutical industry. Therefore, we developed a novel strategy for reducing side effects in cancer patients by providing a novel food supplement with the taste-modifying glycoprotein miraculin, which is approved by the European Union, as an adjuvant to medical–nutritional therapy. Methods: A pilot randomized, parallel, triple-blind, and placebo-controlled intervention clinical trial was carried out in which 31 malnourished patients with cancer and dysgeusia receiving antineoplastic treatment were randomized into three arms—standard dose of dried miracle berries (DMBs) (150 mg DMB/tablet), high dose of DMBs (300 mg DMB/tablet), or placebo (300 mg freeze-dried strawberry)—for three months. Patients consumed a DMB or placebo tablet before each main meal (breakfast, lunch, and dinner). Using stool samples from patients with cancer, we analyzed the intestinal microbiome via nanopore methodology. Results: We detected differences in the relative abundances of genera Phocaeicola and Escherichia depending on the treatment. Nevertheless, only the Solibaculum genus was more abundant in the standard-dose DMB group after 3 months. At the species level, Bacteroides sp. PHL 2737 presented a relatively low abundance in both DMB groups, whereas Vescimonas coprocola presented a relatively high abundance in both treatment groups after 3 months. Furthermore, a standard dose of DMB was positively associated with TNF-α levels and Lachnoclostridium and Mediterraneibacter abundances, and a high dose of DMB was negatively associated with TNF-α levels and the relative abundance of Phocaeicola. Following the administration of a high dose of DMB, a positive correlation was observed between erythrocyte polyunsaturated fatty acids and the presence of Lachnoclostridium and Roseburia. Additionally, a positive association was identified between Phocaeicola and the acetic acid concentration of feces. There was a negative association between the relative abundance of Phocaeicola and taste perception in the high-dose DMB group. Conclusions: The combination of DMB intake with nutritional treatment and individualized dietary guidance results in positive changes in the intestinal microbiome of patients with cancer and dysgeusia. Changes observed in the intestinal microbiome might contribute to maintaining an appropriate immune response in cancer patients. As the current pilot study included a limited number of participants, further clinical trials on a larger group of patients are needed to draw robust findings.
A Comprehensive Analysis of Echinococcus granulosus Infections in Children and Adolescents: Results of a 7-Year Retrospective Study and Literature Review
Authors: Mihai Cristina Maria , Lupu Ancuta , Chisnoiu Tatiana , Balasa Adriana Luminita , Baciu Ginel , Lupu Vasile Valeriu , Popovici Violeta , Suciu Felicia , Enache Florin-Daniel , Cambrea Simona Claudia , Stoicescu Ramona Mihaela
Published: January 2025

Cystic echinococcosis (CE) is a neglected tropical parasitic disease linked with significant social and economic burdens worldwide. The scientific community has minimal information on echinococcosis in Romanian people, and hospital medical records are the only sources that may be used to investigate its status. A 7-year retrospective clinical study on pediatric patients with CE from Southeast Romania was performed, and 39 children and adolescents were included, aged 2–15 years old. They were hospitalized with cystic echinococcosis in the Pediatric Department and Pediatric Surgery Department of Constanta County Clinical Emergency Hospital “St. Apostle Andrew” between 1 January 2017 and 1 October 2024. Twenty-nine (74.36%) pediatric patients came from rural zones, and 10 (25.64%) had urban residences. In total, 28 children (71.79%) had contact with four different animals (dogs, goats, pigs, and sheep); only four were from urban zones, and they had contact only with dogs. Data regarding the length of hospital stay, cyst location, and complications were collected and analyzed. According to the medical files, the diagnosis was established using imaging techniques and serological tests for CE. IgE and IgG reported appreciable variations in correlation with all parameters, and significant differences (p < 0.05) were recorded. IgE levels considerably increased in cases of no animal contact, pulmonary involvement, complications, surgical treatment, and multiple hospitalizations. Moderate IgE values were recorded in cases of urban residences, pig and sheep contact, and hepatic involvement. The IgG concentration considerably increased with sheep contact and moderately increased in cases of rural zones, hepatic involvement, complications, and surgical treatment. The results show that incidental discovery, symptoms, complications, multiple dissemination, pulmonary involvement, and dog and pig contact increase the hospitalization time. Extensive data analysis supports our results. Our findings highlight the complexity of managing E. granulosus infections in children and evidence the importance of a multidisciplinary approach, combining early diagnostic tools, tailored medical therapy, and careful surgical intervention when necessary.
Development and Characterization of Lyophilized Chondroitin Sulfate-Loaded Solid Lipid Nanoparticles: Encapsulation Efficiency and Stability
Authors: Bustos Araya Marta E. , Ricart Anna Nardi , Calpena Campmany Ana C. , Prohens Rafel , Miñarro Carmona Montserrat
Published: January 2025

This study explores the development and characterization of lyophilized chondroitin sulfate (CHON)-loaded solid lipid nanoparticles (SLN) as an innovative platform for advanced drug delivery. Background/Objectives: Solid lipid nanoparticles are increasingly recognized for their biocompatibility, their ability to encapsulate diverse compounds, their capacity to enhance drug stability, their bioavailability, and their therapeutic efficacy. Methods: CHON, a naturally occurring glycosaminoglycan with anti-inflammatory and regenerative properties, was integrated into SLN formulations using the hot microemulsion technique. Two formulations (SLN-1 and SLN-2) were produced and optimized by evaluating critical physicochemical properties such as particle size, zeta potential, encapsulation efficiency (EE%), and stability. The lyophilization process, with the addition of various cryoprotectants, revealed trehalose to be the most effective agent in maintaining nanoparticle integrity and functional properties. Results: Morphological analyses using transmission electron microscopy (TEM) and atomic force microscopy (AFM) confirmed the dimensions of the nanoscales and their structural uniformity. Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) revealed minimal excipient interaction with CHON, ensuring formulation stability. Stability studies under different environmental conditions highlighted that SLN-2 is the most stable formulation, maintaining superior encapsulation efficiency (≥88%) and particle size consistency over time. Conclusions: These findings underscore the potential of CHON-loaded SLNs as promising candidates for targeted, sustained-release therapies in the treatment of inflammatory and degenerative diseases.
Response of Plant Leaf Traits to Environmental Factors in Climax Communities at Varying Latitudes in Karst Regions
Authors: Xie Gang , Wang Yang , Chen Zhifeng , Ji Yangyang , Lu Yao , Liang Yi , Zhou Ruiyu , Tao Juan
Published: January 2025

Exploring the changes in plant functional traits and their relationship with the environment in karst climax communities across different latitudes can enhance our understanding of how these communities respond to environmental gradients. In this study, we focus on climax karst climax plant communities in Guizhou Province, China. We selected three sample sites located at varying latitudes and analyzed the variations in functional traits of the plant communities at these latitudes. Additionally, we examined the relationship between functional traits and environmental factors, integrating species characteristics and community structure into our analysis. The results indicated that (1) there were significant differences in both the community leaf aspect ratio and the community-specific leaf area. (2) Soil organic carbon content exhibited significant variations across different latitudes, while soil nitrogen content was notably higher in mid-latitude and low-latitude regions compared to high-latitude areas. The distribution of soil factors was more concentrated in high and mid-latitude regions, whereas low-latitude areas displayed more pronounced variability. (3) The primary environmental factors influencing the climax community in the karst study area included soil water content (SPMC), soil bulk density (BD), soil organic carbon content (SOC), soil nitrogen content (SNC), and soil phosphorus content (SPC). Our findings suggest that karst plant communities exhibit specific combinations of functional traits at distinct latitudes. With increasing latitude, the community demonstrated a gradual shift in ecological strategy from conservative to more opportunistic. Most environmental factors imposed limiting effects on plant functional traits, with plants primarily constrained by BD during growth. Among the responses of plant functional traits to environmental factors, community-weighted leaf area and community-weighted chlorophyll content were the most sensitive to soil conditions.