bluuebunny/crossref_metadata_2025 huggingface dataset:
Developing Social Entrepreneurship Orientation: The Impact of Internal Work Locus of Control and Bricolage
Authors: Xiabao Peng , Horsey Emmanuel Mensah , Song Xiaofan , Guo Rui
Published: May 2022

Using core self-evaluation theory, the current study assesses the effect of internal work locus of control and bricolage on social entrepreneurship orientation. We adopted the cross-sectional survey design using a sampling frame to engage 400 top executives of social enterprises in mainland China. Three hundred and seventy-two of the executives replied, presenting a response rate of 93%. Results of structural equation modeling analysis show significant positive relationships between internal work locus of control, bricolage, and social entrepreneurship orientation. The positive mediating effect of bricolage on the relationship between internal work locus of control and social entrepreneurship orientation was also found to be true. Consequently, to foster social entrepreneurship orientation, top executives of social enterprises need to gather available resources for bricolage tasks. These findings contribute new knowledge to how internal work locus of control affects social entrepreneurship orientation through the bricolage activity of Chinese social enterprises. Through core self-evaluation theory, we demonstrate the effect of internal work locus of control as a preceding factor in the relationship between bricolage and social entrepreneurship orientation.
Association Between History of Gestational Diabetes Mellitus and the Risk of Arthritis in Women
Authors: Mao Yuanyuan , Hu Wenbin , Xia Bin , Liu Li , Liu Qin
Published: May 2022

Objective

The association between gestational diabetes mellitus (GDM) and the risk of arthritis has not been reported. GDM increases the risk of long-term complications including diabetes and metabolic syndrome that are positively associated with the risk of arthritis. This study aimed to explore the association between GDM and the risk of arthritis.

Methods

Women (age ≥ 20 years) who had delivered at least one live birth were included from the 2007 to 2018 National Health and Nutrition Examination Survey cohort (N = 11,997). Patients who had a history of GDM and arthritis were identified by in-home interview. Subgroup analyses were conducted by arthritis types and status of obesity, current diabetes, metabolic syndrome, smoking, alcohol drinking, and physical activity.

Results

GDM was associated with increased odds of arthritis [multivariable-adjusted odds ratio (95% confidence interval): 1.31 (1.06–1.62)], and the result was similar in sensitivity analysis with further adjustment for metabolic syndrome [1.30 (1.05–1.60)]. In subgroup analyses, GDM was associated with increased odds of osteoarthritis [1.47 (1.05–2.06)], while no association was observed with rheumatoid arthritis [1.04 (0.69–1.57)] and other types [1.26 (0.94–1.68)]. GDM was associated with increased odds of arthritis in women without metabolic syndrome [1.34 (1.00–1.78)] and diabetes [1.35 (1.03–1.76)], in obese individuals [1.64 (1.24–2.16)], current/former smokers [1.43 (1.05–1.95)], and current drinkers [1.76 (1.00–3.14)], and in individuals engaging in higher levels of physical activity [1.53 (1.06–2.20)].

Conclusions

GDM was associated with increased odds of arthritis, and the association was independent of type 2 diabetes and metabolic syndrome.
A Prediction Model for Peak Expiratory Flow Derived From Venous Blood Biomarkers and Clinical Factors in Amyotrophic Lateral Sclerosis
Authors: He Xianghua , Feng Jiaming , Cong Xue , Huang Hongyan , Zhao Quanzhen , Shen Qiuyan , Xu Fang , Xu Yanming
Published: May 2022

Although peripheral venous blood biomarkers are related to respiratory function in Amyotrophic lateral sclerosis (ALS) patients, there are still few prediction models that predict pulmonary function. This study aimed to investigate the venous blood biomarkers associated with respiratory function in patients with ALS from southwest China and to create prediction models based on those clinical biomarkers using logistic regression. A total of 319 patients with ALS from the retrospective cohort and 97 patients with ALS from the prospective cohort were enrolled in this study. A multivariable prediction model for the correlation between peak expiratory flow (PEF) and hematologic, biochemical laboratory parameters, and clinical factors in patients with ALS was created. Along with female patients, bulbar-onset, lower body mass index (BMI), later age of onset, lower level of creatinine, uric acid, triglyceride, and a higher level of high-density lipoprotein cholesterol (HDL_C) were related to reduced PEF. The area under the receiver operating characteristics (ROC) curve is.802 for the test set and.775 for the validation set. The study constructed a multivariable prediction model for PEF in patients with ALS. The results can be helpful for clinical practice to predict respiratory impairment.
Optimally Temperature Compensated FBG-Based Sensor Dedicated to Non-Intrusive Pipe Internal Pressure Monitoring
Authors: Maurin Laurent , Roussel Nicolas , Laffont Guillaume
Published: May 2022

Pipe internal pressure measurement is of utmost importance in the oil & gas industry to monitor the extraction process, and thus to prevent hydrate-plugs formation which may occur in specific temperature and pressure conditions. Traditional solutions usually rely on pressure sensors in direct contact with the fluid to monitor, therefore requiring one hole per sensor, but they also weaken the pipe structure, which may prematurely lead to significant leaks. Attempts to develop non-intrusive pressure sensors relying, for instance, on acoustic waves detection or even strain measurements (the pipe wall acting, in some way, like the membrane of a traditional intrusive sensor), are up to now not fully satisfying, mainly due to poor temperature cross-sensitivity compensation. Thus, 1 °C temperature compensation error typically leads for Fiber Bragg Grating (FBG) transducers to pressure measurement biases greater than 26% at 100 bar (e.g.: Ø 4” NPS Sch. 160 steel pipe). Consequently, if such non-intrusive, but biased, solutions could possibly have been considered to monitor, for instance, a Nuclear Power Plant (NPP) primary coolant circuit, it was with the risk of dramatic consequences since the fluid can reach temperatures up to 320 °C. On the other hand, the solution detailed here truly achieves to cancel the temperature cross-sensitivity, and potentially any additional effect on pressure measurement, provided that each effect has the same influence on all transducers. It first relies on a better understanding of the pipe behavior under hydrostatic pressure, supported by a dedicated model developed on purpose, which demonstrates that the internal pressure and the surface temperature variations of a closed pipe can be recovered with at least two direction-sensitive transducers, the temperature dependence of the pressure measurement being simply removed by a straightforward compensation process. This paper explains the underlying principle, thanks to a formal model established with only few hypotheses, but extended to more complex field conditions. It ends with a lab-test validation involving FBG transducers attached to a pressure circuit submitted to temperature variations greater than several tens of °C, and concludes about the advantages and limitations of this novel approach for non-intrusive sensing, and its potential extensions to other measurement techniques.
Case Report: 3D-Printed Prosthesis for Limb Salvage and Joint Preservation After Tibial Sarcoma Resection
Authors: Guo Zehao , Zhang Ran , Que Yukang , Hu Bo , Xu Shenglin , Hu Yong
Published: May 2022

Introduction

Reconstruction of massive tibial defects in ankle joint-preserving surgery remains challenging though biological and prosthetic methods have been attempted. We surgically treated a patient with only 18-mm distal tibia remaining and reconstructed with a unique three-dimensional printed prosthesis.

Case Presentation, Intervention, and Outcomes

A 36-year-old male presented to our clinic with complaints of gradually swelling left calf and palpable painless mass for five months. Imageological exam indicated a lesion spanning the entire length of the tibia and surrounding the vascular plexus. Diagnosis of chondrosarcoma was confirmed by biopsy. Amputation was initially recommended but rejected, thus a novel one-step limb-salvage procedure was performed. After en-bloc tumor resection and blood supply rebuilding, a customized, three-dimensional printed prosthesis with porous interface was fixed that connected the tumor knee prosthesis and distal ultra-small bone segment. During a 16-month follow-up, no soft tissue or prosthesis-related complications occurred. The patient was alive with no sign of recurrence or metastasis. Walking ability and full tibiotalar range of motion were preserved.

Conclusions

Custom-made, three-dimensional printed prosthesis manifested excellent mechanical stability during the follow-up in this joint-preserving surgery. Further investigation of the durability and rate of long-term complications is needed to introduce to routine clinical practice.
Comparison of Dynamics of Udder Skin Microbiota From Grazing Yak and Cattle During the Perinatal Period on the Qinghai–Tibetan Plateau
Authors: Zhang Jianbo , Ahmad Anum Ali , Jia Yan , Dingkao Renqing , Du Mei , Liang Zeyi , Zheng Juanshan , Bature Ibrahim , Yan Ping , Salekdeh Ghasem Hosseini , Ding Xuezhi
Published: May 2022

The perinatal period has an important impact on the health of ruminants, and the imbalance of udder skin microbiota might be an important inducement of bovine mastitis. However, it is not clear how the perinatal period affects the microbial structure and stability of the udder skin of yak and cattle. Here, we used 16S rRNA gene high-throughput sequencing to analyze the udder skin microbiota of yak and cattle during the perinatal period. We found that the diversity and richness of microbiota of bovine udder skin during 1–2 weeks postpartum were significantly lower than those in the 1–2 weeks prenatal and 1-month postpartum period (Wilcoxon, p < 0.05). Besides, we found sharing of 2,533 OTUs in the udder skin microbiota of yak and cattle during the perinatal period, among which the core microbiota at the genera level was mainly composed of Staphylococcus, Moraxella, and Acinetobacter. However, the genus Acinetobacter was significantly abundant in the udder skin of cattle during 1–2 weeks postpartum. The NMDS and LEfSe results showed that the perinatal period had more effects on the composition and stability of microbial community in the udder skin of cattle compared to yak, particularly during 1–2 weeks postpartum. In addition, the average content of total whey proteins and immunoglobulin G of whey protein were significantly higher in the yak colostrum when compared to those found in the cattle (p < 0.05). In conclusion, the structure of udder skin microbiota of yak during the perinatal period is more stable than that of cattle in the same habitat, and 1–2 weeks postpartum may be a potential window period to prevent cattle mastitis.
Welfare Concerns for Mounted Load Carrying by Working Donkeys in Pakistan
Authors: Bukhari Syed S. U. H. , Rosanowski Sarah M. , McElligott Alan G. , Parkes Rebecca S. V.
Published: May 2022

Working donkeys (Equus asinus) are vital to people's livelihoods. They are essential for carrying goods, however, globally, overloading is one of the primary welfare concerns for working donkeys. We studied mounted load carrying by donkeys and associated factors in Pakistan. A cross-sectional study of donkey owners (n = 332) was conducted, and interviews were undertaken based on a questionnaire. Owners estimated that the median weight of their donkeys was 110 kg [interquartile range (IQR) 100–120 kg], and that they carried a median mounted load of 81.5 kg (IQR 63–99 kg). We found that 87.4% of donkeys carried a load above 50% of their bodyweight ratio (BWR), the median BWR carried was 77.1% (IQR 54.5–90.7%), and 25.3% of donkeys carried above 90% BWR. Donkeys that were loaded at more than 50% BWR were more likely to adopt sternal recumbency compared to donkeys loaded with less weight (P = 0.01). Donkeys carrying construction material were more likely to carry more than the median BWR, when compared to domestic loads (P < 0.001). Younger donkeys aged between one and 5 years carried more than the median BWR compared to those aged over 15 years (P = 0.03). For the models with donkeys carrying median BWR and above 90% BWR, those working in peri-urban and urban areas were more likely to carry a greater BWR than donkeys working in rural areas (P < 0.001; P < 0.001, respectively). For donkeys carrying more than 90% BWR, mixed breed donkeys carried higher loads compared to other breeds of donkeys (P < 0.001). Overloading based on current recommendations (50% BWR) was common, with the majority (87.4%) of donkeys reported to carry more than the recommended 50% limit. This survey provides evidence of on-the-ground working practices and factors associated with mounted load carrying, which is critical for developing evidence-based recommendations for loading, in order to improve the welfare of working donkeys.
The Prevalence of Several Risky Driving Behaviors and Associated Crash Risk in Adolescent: A Population-Based Study of Tuscany Region
Authors: Lastrucci Vieri , Innocenti Francesco , Lorini Chiara , Berti Alice , Silvestri Caterina , Lazzeretti Marco , Voller Fabio , Bonaccorsi Guglielmo
Published: May 2022

Objectives: To evaluate the prevalence of numerous risky driving behaviors (RDBs) and the associated risk of road traffic accidents (RTA) in a population-based sample of adolescent drivers (14–19 years) of Tuscany, Italy.

Methods: The frequency of participation -by age and sex- often RDBs were investigated: Multivariable analyses were performed to evaluate the association between RDBs and the risk of RTA and severe RTA.

Results: 2,737 adolescents were included in the study. Talking to passenger(s), listening to loud music, speeding, and texting showed the highest weekly participation rates. For all the considered RDBs, the frequency of participation significantly increased with age. Males reported a significantly higher participation in speeding, DUI of alcohol or drugs; while females reported listening to loud music and talking to passenger(s) more frequently. All the considered RDBs were significantly associated with the risk of RTA and severe RTA.

Conclusion: The prevalence of RDBs and the associated risk of RTA largely varied in adolescents. Findings provide evidence for tailoring prevention interventions and suggest the need to include common- but traditionally overlooked- RDBs in road safety campaigns.
Hospital-Based Health Technology Assessment of Machine Perfusion Systems for Human Liver Transplantation
Authors: De Simone Paolo , Ghinolfi Davide
Published: May 2022

Based on published data, we have carried out a hospital-based health technology assessment of machine perfusion in adult liver transplantation using cold storage as a comparator, and within the perspective of a national health system-based hospital practice and disease-related group reimbursement policy. A systematic literature review on machine perfusion for adult liver transplantation was conducted exploring the Pubmed, CINAHL, Scopus, Embase, and Cochrane databases. The literature was analyzed with the intent to provide information on 6 dimensions and 19 items of the hospital-based health technology assessment framework derived from previous studies. Out of 705 references, 47 (6.7%) were retained for current analysis. Use of machine perfusion was associated with advantages over cold storage, i.e., a 10%–50% reduced risk for early allograft dysfunction, 7%–15% less ischemia reperfusion injury; 7%–50% fewer ischemic biliary complications, comparable or improved 1-year graft and patient survival, and up to a 50% lower graft discard rate. Hospital stay was not longer, and technical failures were anecdotal. Information on costs of machine perfusion is limited, but this technology is projected to increase hospital costs while cost-effectiveness analysis requires data over the transplant patient lifetime. No hospital-based health technology assessment study on machine perfusion in liver transplantation was previously conducted. From the hospital perspective, there is evidence of the clinical advantages of this novel technology, but strategies to counterbalance the increased costs of liver transplantation are urgently needed. Further studies should focus on the ethical, social, and organizational issues related to machine perfusion.
Exploring the Suitability of Rule-Based Classification to Provide Interpretability in Outcome-Based Process Predictive Monitoring
Authors: Lee Suhwan , Comuzzi Marco , Kwon Nahyun
Published: May 2022

The development of models for process outcome prediction using event logs has evolved in the literature with a clear focus on performance improvement. In this paper, we take a different perspective, focusing on obtaining interpretable predictive models for outcome prediction. We propose to use association rule-based classification, which results in inherently interpretable classification models. Although association rule mining has been used with event logs for process model approximation and anomaly detection in the past, its application to an outcome-based predictive model is novel. Moreover, we propose two ways of visualising the rules obtained to increase the interpretability of the model. First, the rules composing a model can be visualised globally. Second, given a running case on which a prediction is made, the rules influencing the prediction for that particular case can be visualised locally. The experimental results on real world event logs show that in most cases the performance of the rule-based classifier (RIPPER) is close to the one of traditional machine learning approaches. We also show the application of the global and local visualisation methods to real world event logs.
Anaemia in Lambs Caused by Mycoplasma ovis: Global and Australian Perspectives
Authors: Windsor Peter
Published: May 2022

Mycoplasma ovis (formerly Eperythrozoon ovis) is a haemotropic parasitic bacterium found within erythrocytes and distributed widely in global sheep and goat production regions. M. ovis is transmitted by biting flies and by contaminated instruments, causing morbidity and mortalities from anaemia, usually within 6 weeks following blood-exposure procedures, particularly vaccination, castration, ear tagging, mulesing, and occasionally crutching and shearing. Affected animals develop haemolytic anaemia and may have jaundice, causing abattoir condemnations. The typical history, clinical and pathological findings, display of M. ovis in blood smears and/or by PCR is diagnostic, although immune responses deplete M. ovis from smears; hence, in-contact healthy animals should be examined. Differential diagnoses include haemonchosis, fasciolosis, malnutrition (copper or vitamin B12 deficiency), and plant toxicities. M. ovis parasitaemia may persist, with recrudescence following stressful events, although most older sheep remain immune. Human infections have been reported. Inadequate socioeconomic data present difficulties in assessing the impact of M. ovis on production and as antimicrobial therapy is ineffective, its control requires management practices that minimize the impact of invasive procedures in periods when risks of M. ovis transmission are more likely. Although considered an emerging pathogen, recent improvements in welfare attitudes and husbandry practices on Australian sheep farms may potentially limit the transmission of M. ovis.
Growth Curves and Body Condition of Young Cats and Their Relation to Maternal Body Condition
Authors: Opsomer Han , Liesegang Annette , Brugger Daniel , Wichert Brigitta
Published: May 2022

The aim of the present study was to assess factors like litter, individual and maternal effects on kitten overweight at 8 months of age, defined as body condition score (BCS) ≥ 6, in an intact cat family. To minimize confounding, a homogenized cat population was used. After categorization of the life weight data according to the kittens’ sex, BCS and maternal non-pregnant phenotype (overweight (OM), lean (LM), variable (VM)), analyses including Pearson’s correlation coefficients, two-way ANOVA, linear, linear broken-line regression and repeated measures mixed model analyses were performed. Overweight and OM kittens gained weight most quickly, and females reached their peak weight earlier than males (6.2 ± 0.6 vs. 7.4 ± 0.2 months). In females but not in males the age to reach peak weight differed markedly according to category. Male (5.82 ± 0.09, p < 0.01) and OM kittens’ (5.80 ± 0.11, p = 0.07) BCS at 8 months was higher and they were heavier than their counterparts, from 3 and 5 months onwards, respectively. Litter size negatively correlated with overweight (r = −0.30, p < 0.01) and birthweight showed a positive correlation to live weight (R2 = 0.05, p = 0.05) and monthly weight gain (R2 = 0.18, p < 0.01) over time. This study supports routine monitoring of birthweight, growth rate and maternal phenotype prior to pregnancy to identify kittens at risk for becoming overweight.
Endoscopic and Surgical Removal of Gastrointestinal Foreign Bodies in Dogs: An Analysis of 72 Cases
Authors: Di Palma Cristina , Pasolini Maria Pia , Navas Luigi , Campanile Andrea , Lamagna Francesco , Fatone Gerardo , Micieli Fabiana , Esposito Ciro , Donnarumma Daniela , Uccello Valeria , Lamagna Barbara
Published: May 2022

In emergency veterinary practice, gastrointestinal foreign body (GFB) removal is a common procedure that is performed with different techniques, such as endoscopy or surgery. The aims of this retrospective, multicentre, clinical study were to report the common locations and types of objects recovered and to investigate clinical factors and outcomes in dogs after surgical or endoscopic treatment for GFB removal. Records of dogs with a GFB diagnosis referred to the Teaching Veterinary Hospital or treated in three different veterinary hospitals from September 2017 to September 2019 were examined. The data obtained from each case included breed, age, clinical signs at presentation, duration of clinical signs, type and location of the GFB, treatment, length of hospitalisation and outcome. Seventy-two dogs were enrolled in the study. There were 42 males (58%) and 30 females (42%). The median age was 36 months (range: 3 months to 8 years). Endoscopic retrieval was performed in 56% of GFBs (located in the stomach or duodenum), whereas 44% of dogs underwent surgery. The type of FB detected varied greatly: kid toy (14%), metallic object/coin (13%), cloth (13%), sock (8%), ball (8%), plastic material (8%), peach stone (7%), fishhook (6%), sewing needle (4%), hair tie (4%), pacifier (3%), plant materials (3%) and others (9%). Moreover, the FBs were classified as sharp (13%, n = 9), pointed (33%, n = 24), blunt (26%, n = 19), or linear (28%, n = 20). In this study, 68% of FBs were localised in the stomach, 25% in the intestinal tract (50% duodenum, 28% jejunum, and 22% ileum), and 7% in both the stomach and small intestine. The type of GFB was not significantly associated with age, site or breed. There was a significant association between the type of GFB and sex: if the dog was male, there was a 38% probability of ingesting linear GFBs. The dog survival rate was 100% in cases treated by gastric endoscopic or surgical removal, 94% in cases treated with enterotomy and 33% in cases in which enterectomy was necessary. Enterectomy and multiple surgical sites were associated with a poor outcome. The presence of vomiting for more than 24 h was significantly associated with death.
Impact of Full Prelithiation of Si-Based Anodes on the Rate and Cycle Performance of Li-Ion Capacitors
Authors: Eguchi Takuya , Sugawara Ryoichi , Abe Yusuke , Tomioka Masahiro , Kumagai Seiji
Published: May 2022

The impact of full prelithiation on the rate and cycle performance of a Si-based Li-ion capacitor (LIC) was investigated. Full prelithiation of the anode was achieved by assembling a half cell with a 2 µm-sized Si anode (0 V vs. Li/Li+) and Li metal. A three-electrode full cell (100% prelithiation) was assembled using an activated carbon (AC) cathode with a high specific surface area (3041 m2/g), fully prelithiated Si anode, and Li metal reference electrode. A three-electrode full cell (87% prelithiation) using a Si anode prelithiated with 87% Li ions was also assembled. Both cells displayed similar energy density levels at a lower power density (200 Wh/kg at ≤100 W/kg; based on the total mass of AC and Si). However, at a higher power density (1 kW/kg), the 100% prelithiation cell maintained a high energy density (180 Wh/kg), whereas that of the 87% prelithiation cell was significantly reduced (80 Wh/kg). During charge/discharge cycling at ~1 kW/kg, the energy density retention of the 100% prelithiation cell was higher than that of the 87% prelithiation cell. The larger irreversibility of the Si anode during the initial Li-ion uptake/release cycles confirmed that the simple full prelithiation process is essential for Si-based LIC cells.
Bioengineered Wound Healing Skin Models: The Role of Immune Response and Endogenous ECM to Fully Replicate the Dynamic of Scar Tissue Formation In Vitro
Authors: Urciuolo Francesco , Passariello Roberta , Imparato Giorgia , Casale Costantino , Netti Paolo Antonio
Published: May 2022

The healing of deep skin wounds is a complex phenomenon evolving according with a fine spatiotemporal regulation of different biological events (hemostasis, inflammation, proliferation, remodeling). Due to the spontaneous evolution of damaged human dermis toward a fibrotic scar, the treatment of deep wounds still represents a clinical concern. Bioengineered full-thickness skin models may play a crucial role in this direction by providing a deep understanding of the process that leads to the formation of fibrotic scars. This will allow (i) to identify new drugs and targets/biomarkers, (ii) to test new therapeutic approaches, and (iii) to develop more accurate in silico models, with the final aim to guide the closure process toward a scar-free closure and, in a more general sense, (iv) to understand the mechanisms involved in the intrinsic and extrinsic aging of the skin. In this work, the complex dynamic of events underlaying the closure of deep skin wound is presented and the engineered models that aim at replicating such complex phenomenon are reviewed. Despite the complexity of the cellular and extracellular events occurring during the skin wound healing the gold standard assay used to replicate such a process is still represented by planar in vitro models that have been largely used to identify the key factors regulating the involved cellular processes. However, the lack of the main constituents of the extracellular matrix (ECM) makes these over-simplistic 2D models unable to predict the complexity of the closure process. Three-dimensional bioengineered models, which aim at recreating the closure dynamics of the human dermis by using exogenous biomaterials, have been developed to fill such a gap. Although interesting mechanistic effects have been figured out, the effect of the inflammatory response on the ECM remodelling is not replicated yet. We discuss how more faithful wound healing models can be obtained by creating immunocompetent 3D dermis models featuring an endogenous ECM.
NF-κB: A Double-Edged Sword Controlling Inflammation
Authors: Liu Danhui , Zhong Zhenyu , Karin Michael
Published: May 2022

Inflammation, when properly mounted and precisely calibrated, is a beneficial process that enables the rapid removal of invading pathogens and/or cellular corpses and promotes tissue repair/regeneration to restore homeostasis after injury. Being a paradigm of a rapid response transcription factor, the nuclear factor-kappa B (NF-κB) transcription factor family plays a central role in amplifying inflammation by inducing the expression of inflammatory cytokines and chemokines. Additionally, NF-κB also induces the expression of pro-survival and -proliferative genes responsible for promoting tissue repair and regeneration. Paradoxically, recent studies have suggested that the NF-κB pathway can also exert inhibitory effects on pro-inflammatory cytokine production to temper inflammation. Here, we review our current understanding about the pro- and anti-inflammatory roles of NF-κB and discuss the implication of its dichotomous inflammation-modulating activity in the context of inflammasome activation and tumorigenesis.
Macrophage-Derived Extracellular Vesicles: A Promising Tool for Personalized Cancer Therapy
Authors: Barone Antonella , d’Avanzo Nicola , Cristiano Maria Chiara , Paolino Donatella , Fresta Massimo
Published: May 2022

The incidence of cancer is increasing dramatically, affecting all ages of the population and reaching an ever higher worldwide mortality rate. The lack of therapies’ efficacy is due to several factors such as a delay in diagnosis, tumor regrowth after surgical resection and the occurrence of multidrug resistance (MDR). Tumor-associated immune cells and the tumor microenvironment (TME) deeply affect the tumor’s progression, leading to several physicochemical changes compared to physiological conditions. In this scenario, macrophages play a crucial role, participating both in tumor suppression or progression based on the polarization of onco-suppressive M1 or pro-oncogenic M2 phenotypes. Moreover, much evidence supports the pivotal role of macrophage-derived extracellular vesicles (EVs) as mediators in TME, because of their ability to shuttle the cell–cell and organ–cell communications, by delivering nucleic acids and proteins. EVs are lipid-based nanosystems with a broad size range distribution, which reflect a similar composition of native parent cells, thus providing a natural selectivity towards target sites. In this review, we discuss the impact of macrophage-derived EVs in the cancer’s fate as well as their potential implications for the development of personalized anticancer nanomedicine.
Follicular Fluid: A Powerful Tool for the Understanding and Diagnosis of Polycystic Ovary Syndrome
Authors: Brinca Ana Teresa , Ramalhinho Ana Cristina , Sousa Ângela , Oliani António Hélio , Breitenfeld Luiza , Passarinha Luís A. , Gallardo Eugenia
Published: May 2022

Polycystic ovary syndrome (PCOS) represents one of the leading causes of anovulatory infertility and affects 5% to 20% of women worldwide. Until today, both the subsequent etiology and pathophysiology of PCOS remain unclear, and patients with PCOS that undergo assisted reproductive techniques (ART) might present a poor to exaggerated response, low oocyte quality, ovarian hyperstimulation syndrome, as well as changes in the follicular fluid metabolites pattern. These abnormalities originate a decrease of Metaphase II (MII) oocytes and decreased rates for fertilization, cleavage, implantation, blastocyst conversion, poor egg to follicle ratio, and increased miscarriages. Focus on obtaining high-quality embryos has been taken into more consideration over the years. Nowadays, the use of metabolomic analysis in the quantification of proteins and peptides in biological matrices might predict, with more accuracy, the success in assisted reproductive technology. In this article, we review the use of human follicular fluid as the matrix in metabolomic analysis for diagnostic and ART predictor of success for PCOS patients.
Apoptosis and (in) Pain—Potential Clinical Implications
Authors: Ribeiro Hugo , Sarmento-Ribeiro Ana Bela , Andrade José Paulo , Dourado Marília
Published: May 2022

The deregulation of apoptosis is involved in the development of several pathologies, and recent evidence suggests that apoptosis may be involved in chronic pain, namely in neuropathic pain. Neuropathic pain is a chronic pain state caused by primary damage or dysfunction of the nervous system; however, the details of the molecular mechanisms have not yet been fully elucidated. Recently, it was found that nerve endings contain transient receptor potential (TRP) channels that sense and detect signals released by injured tissues and respond to these damage signals. TRP channels are similar to the voltage-gated potassium channels or nucleotide-gated channels that participate in calcium and magnesium homeostasis. TRP channels allowing calcium to penetrate into nerve terminals can activate apoptosis, leading to nerve terminal destruction. Further, some TRPs are activated by acid and reactive oxygen species (ROS). ROS are mainly produced in the mitochondrial respiratory chain, and an increase in ROS production and/or a decrease in the antioxidant network may induce oxidative stress (OS). Depending on the OS levels, they can promote cellular proliferation and/or cell degeneration or death. Previous studies have indicated that proinflammatory cytokines, such as tumor necrosis factor-α (TNF-α), play an important role in the peripheral mediation of neuropathic pain. This article aims to perform a review of the involvement of apoptosis in pain, particularly the role of OS and neuroinflammation, and the clinical relevance of this knowledge. The potential discovery of new biomarkers and therapeutic targets can result in the development of more effective and targeted drugs to treat chronic pain, namely neuropathic pain. Highlights: Oxidative stress and neuroinflammation can activate cell signaling pathways that can lead to nerve terminal destruction by apoptosis. These could constitute potential new pain biomarkers and targets for therapy in neuropathic pain.
Extrusion and Subsequent Transformation of Textile-Reinforced Mortar Components—Requirements on the Textile, Mortar and Process Parameters with a Laboratory Mortar Extruder (LabMorTex)
Authors: Kalthoff Matthias , Raupach Michael , Matschei Thomas
Published: May 2022

To produce defect-free extruded and shaped components, the forming behaviour of extruded fibre-reinforced mortar mixtures, impregnated textiles and extruded textile-reinforced mortar (TRM) was investigated. The TRM test specimens were formed longitudinally and transversely using specially developed forming setups. Regardless of the selected fibre content ranging between 0 and 0.5 Vol.-%, defect-free longitudinal forming of the mortar is possible up to a bending radius of 5 cm and transversely up to a bending radius of approx. 6 cm. For the extruded TRM specimens, longitudinal bending radii of up to 10 cm were achieved. The results represent the basis for the construction of new formwork-free extruded and subsequently shaped textile-reinforced concrete components.
Emerging Roles of Immune Cells in Cancer Development and Progression
Authors: Entenberg David , Filippou Panagiota S. , Karagiannis George S.
Published: May 2022

Immune cells are a major constituent of the tumor microenvironment, and participate in interactions with tumor cells to promote the acquisition of critical hallmarks of cancer [...]
Gallic Acid: A Natural Phenolic Compound Exerting Antitumoral Activities in Colorectal Cancer via Interaction with G-Quadruplexes
Authors: Sanchez-Martin Victoria , Plaza-Calonge María del Carmen , Soriano-Lerma Ana , Ortiz-Gonzalez Matilde , Linde-Rodriguez Angel , Perez-Carrasco Virginia , Ramirez-Macias Inmaculada , Cuadros Marta , Gutierrez-Fernandez Jose , Murciano-Calles Javier , Rodríguez-Manzaneque Juan Carlos , Soriano Miguel , Garcia-Salcedo Jose Antonio
Published: May 2022

Natural phenolic compounds have gained momentum for the prevention and treatment of cancer, but their antitumoral mechanism of action is not yet well understood. In the present study, we screened the antitumoral potential of several phenolic compounds in a cellular model of colorectal cancer (CRC). We selected gallic acid (GA) as a candidate in terms of potency and selectivity and extensively evaluated its biological activity. We report on the role of GA as a ligand of DNA G-quadruplexes (G4s), explaining several of its antitumoral effects, including the transcriptional inhibition of ribosomal and CMYC genes. In addition, GA shared with other established G4 ligands some effects such as cell cycle arrest, nucleolar stress, and induction of DNA damage. We further confirmed the antitumoral and G4-stabilizing properties of GA using a xenograft model of CRC. Finally, we succinctly demonstrate that GA could be explored as a therapeutic agent in a patient cohort with CRC. Our work reveals that GA, a natural bioactive compound present in the diet, affects gene expression by interaction with G4s both in vitro and in vivo and paves the way towards G4s targeting with phenolic compounds.
Bottom-Up Approach to the Discovery of Clinically Relevant Biomarker Genes: The Case of Colorectal Cancer
Authors: Kamel Faddy , Schneider Nathalie , Nisar Pasha , Soloviev Mikhail
Published: May 2022

Traditional approaches to genome-wide marker discovery often follow a common top-down strategy, where a large scale ‘omics’ investigation is followed by the analysis of functional pathways involved, to narrow down the list of identified putative biomarkers, and to deconvolute gene expression networks, or to obtain an insight into genetic alterations observed in cancer. We set out to investigate whether a reverse approach would allow full or partial reconstruction of the transcriptional programs and biological pathways specific to a given cancer and whether the full or substantially expanded list of putative markers could thus be identified by starting with the partial knowledge of a few disease-specific markers. To this end, we used 10 well-documented differentially expressed markers of colorectal cancer (CRC), analyzed their transcription factor networks and biological pathways, and predicted the existence of 193 new putative markers. Incredibly, the use of a validation marker set of 10 other completely different known CRC markers and the same procedure resulted in a very similar set of 143 predicted markers. Of these, 138 were identical to those found using the training set, confirming our main hypothesis that a much-expanded set of disease markers can be predicted by starting with just a small subset of validated markers. Further to this, we validated the expression of 42 out of 138 top-ranked predicted markers experimentally using qPCR in surgically removed CRC tissues. We showed that 41 out of 42 mRNAs tested have significantly altered levels of mRNA expression in surgically excised CRC tissues. Of the markers tested, 36 have been reported to be associated with aspects of CRC in the past, whilst only limited published evidence exists for another three genes (BCL2, PDGFRB and TSC2), and no published evidence directly linking genes to CRC was found for CCNA1, SHC1 and TGFB3. Whilst we used CRC to test and validate our marker discovery strategy, the reported procedures apply more generally to cancer marker discovery.
Crosstalk of Epigenetic and Metabolic Signaling Underpinning Glioblastoma Pathogenesis
Authors: Markouli Mariam , Strepkos Dimitrios , Papavassiliou Kostas A. , Papavassiliou Athanasios G. , Piperi Christina
Published: May 2022

Metabolic alterations in neoplastic cells have recently gained increasing attention as a main topic of research, playing a crucial regulatory role in the development and progression of tumors. The interplay between epigenetic modifications and metabolic pathways in glioblastoma cells has emerged as a key pathogenic area with great potential for targeted therapy. Epigenetic mechanisms have been demonstrated to affect main metabolic pathways, such as glycolysis, pentose phosphate pathway, gluconeogenesis, oxidative phosphorylation, TCA cycle, lipid, and glutamine metabolism by modifying key regulatory genes. Although epigenetic modifications can primarily promote the activity of metabolic pathways, they may also exert an inhibitory role. In this way, they participate in a complex network of interactions that regulate the metabolic behavior of malignant cells, increasing their heterogeneity and plasticity. Herein, we discuss the main epigenetic mechanisms that regulate the metabolic pathways in glioblastoma cells and highlight their targeting potential against tumor progression.
Primary High-Grade Myxoid Liposarcoma of the Extremities: Prognostic Factors and Metastatic Pattern
Authors: Tuzzato Gianmarco , Laranga Roberta , Ostetto Federico , Bubbico Elisa , Vara Giulio , Bianchi Giuseppe
Published: May 2022

(1) Background: This retrospective study aimed to analyze the history and treatment outcomes of localized, high-grade MLS of the extremities. (2) Methods: We retrospectively reviewed 82 patients with primary high-grade MLS of the extremities. OS, LRFS, MFS, PRS, and DFS were analyzed. (3) Results: Five-year OS and LRS were 96% (95% CI: 86–98) and 94% (95% CI: 85–98), respectively. Statistical analysis indicated no risk factors for OS and LFRS. MFS was 77% (65–85) at 5-year follow-up. Size (p = 0.0337) was the only risk factor statistically significant for MFS (HR = 0.248, 95% CI: 0.07–0.84). Median PRS after distant metastasis was 34 months (range: 1–127 months). Five-year PRS was 79% (48–93). Overall, the 5-year DFS was 76% (65–85). (4) Conclusions: Patients with MLS were found to have a good prognosis. In high-grade deep-seated tumors, common risk factors for MLS do not correlate with survival. Tumor size appears to be the only predictor of long-term DSF and MSF.
An Integrative Human Pan-Cancer Analysis of Cyclin-Dependent Kinase 1 (CDK1)
Authors: Liu Xuanyou , Wu Hao , Liu Zhenguo
Published: May 2022

Cyclin-dependent kinase 1 (CDK1) is essential for cell division by regulating the G2/M phase and mitosis. CDK1 overexpression can also promote the development and progression of a variety of cancers. However, the significance of CDK1 in the formation, progression, and prognosis of human pan-cancer remains unclear. In the present study, we used The Cancer Genome Atlas database, Clinical Proteomic Tumor Analysis Consortium, Human Protein Atlas, Genotype-Tissue Expression, and other well-established databases to comprehensively examine CDK1 genetic alterations and gene/protein expression in various cancers and their relationships with the prognosis, immune reactivities, and clinical outcomes for 33 tumor types. Gene set enrichment analysis was also conducted to examine the potential mechanisms of CDK1 in tumorigenesis. The data showed that CDK1 mutation was frequently present in multiple tumors. CDK1 expression was significantly increased in various types of tumors as compared with normal tissues and was associated with poor overall and disease-free survival. In addition, CDK1 expression was significantly correlated with oncogenic genes, proteins, cellular components, myeloid-derived suppressor cell infiltration, ESTMATEScore, and signaling pathways associated with tumor development and progression and tumor microenvironments. These data indicate that CDK1 could serve as a promising biomarker for predicting tumor prognosis and a potential target for cancer treatment.
Tropomyosin-Related Kinase Fusions in Gastrointestinal Stromal Tumors
Authors: Lee Ji Hyun , Shin Su-Jin , Choe Eun-Ah , Kim Jungyoun , Hyung Woo Jin , Kim Hyo Song , Jung Minkyu , Beom Seung-Hoon , Kim Tae Il , Ahn Joong Bae , Chung Hyun Cheol , Shin Sang Joon
Published: May 2022

The canonical mutations in gastrointestinal stromal tumors (GISTs) are typically activating mutations in KIT and platelet-derived growth factor receptor alpha (PDGFRA). GISTs with non-canonical mutations are a heterogeneous group. Here, we examined tropomyosin-related kinase (TRK) fusion in GIST cases without KIT/PDGFRA mutations (KIT/PDGFRA wild-type (WT) GISTs). We retrospectively analyzed patients who were diagnosed with GISTs at the Yonsei Cancer Center, Severance Hospital, between January 1998 and December 2016. Thirty-one patients with KIT/PDGFRA WT GISTs were included in the analysis. TRK expression in tumor samples was assessed by pan-TRK immunohistochemistry (IHC), and the neurotrophic tyrosine receptor kinase (NTRK: the gene encoding TRK) rearrangement was analyzed by fluorescence in situ hybridization (FISH). IHC analyses revealed that five cases in this cohort exhibited a weak to moderate TRK expression. NTRK1 fusions were detected in three tumor samples, and two samples harbored NTRK3 fusions. The remaining 26 samples did not harbor NTRK fusions. Two types of NTRK fusions were detected, and the overall NTRK fusion frequency in KIT/PDGFRA WT GIST cases was 16% (5/31). Our data provide insights into the molecular alterations underpinning KIT/PDGFRA WT GISTs. More effort should be devoted to improve methods to identify this distinct disease subtype within the KIT/PDGFRA WT GIST group.
New Paradigms for Cytoreductive Nephrectomy
Authors: Lichtbroun Benjamin J. , Srivastava Arnav , Doppalapudi Sai Krishnaraya , Chua Kevin , Singer Eric A.
Published: May 2022

The role of CN in the treatment of metastatic renal cell carcinoma (mRCC) has been studied over the course of the past few decades. With the advent of immuno-oncologic (IO) agents, there has been a paradigm shift in the treatment of RCC. Within this new era of cancer care, the role of CN is unclear. There are several studies currently underway that aim to assess the role of CN in combination with these therapies. We reviewed articles examining CN, both historically and in the modern immunotherapy era. While immune-oncologic agents are relatively new and large clinical trials have yet to be completed, data thus far is promising that CN may provide clinical benefit. Multiple ongoing trials may clarify the role of CN in this new era of cancer care.
Imaging Endocannabinoids and Bioactive Lipid Messengers in Basic Research and Biomedical Application
Authors: Aoki Jun , Isokawa Masako
Published: May 2022

Endocannabinoids (eCBs) are representative bioactive lipid messengers [...]
A Dual-Frequency Terahertz Metasurface Capable of Distinguishing the Handedness of Circularly Polarized Light
Authors: Yang Bowei , Ouyang Mingzhao , Ren Hang , Ma Chenhao , Zhao Yu , Zhang Yuhui , Fu Yuegang
Published: May 2022

Circularly polarized light can present more optical properties of chiral materials and is widely used to analyze and detect biomolecules. In this paper, a dual-frequency terahertz circular polarization detection structure, which is based on multilayer metamaterials, is proposed. The proposed structure consists of a dual-frequency quarter-wave plate, a polyimide spacer, and a filter. The simulation results show that the structure can distinguish the handedness of circularly polarized light by filtering. The extinction ratios are 4 dB and 5.26 dB at 0.952 THz and 1.03 THz, respectively, and the maximum transmittance efficiency reaches 40%. Given the advantages of easy integration and dual-frequency operation, our design is bound to facilitate the development of multi-frequency detection in biomedical imaging devices.
Preprocessing of Gravity Data
Authors: Izvoltova Jana , Bacova Dasa , Chromcak Jakub , Hodas Stanislav
Published: May 2022

The paper deals with computation techniques applied in preprocessing of gravity data, which are based on time series analysis by using mathematical and statistical smoothing techniques such as moving average, moving median, cumulative and moving average, etc. The main aim of gravity data preprocessing is to avoid abrupt errors caused by a sudden movement of the subsoil due to human or natural activities or systematic instrumental influences and so provide relevant gravity values, which are then subjected to further processing. The new approach of the described research involves the preprocessing phase in gravity data analysis to identify and avoid gross errors, which could influence the size of unknown parameters estimated by the least square method in the processing phase.