bluuebunny/crossref_metadata_2025 huggingface dataset:
Degradation of soybean meal proteins by wheat malt endopeptidase and the antioxidant capacity of the enzymolytic products
Authors: Fan Jingxiao , Gao Aiying , Zhan Chao , Jin Yuhong
Published: March 2023

This study investigated the hydrolysis effect of the endopeptidase from wheat malt on the soybean meal proteins. The results indicated that the endopeptidase broke the peptide bonds of soybean meal proteins and converted the alcohol- and alkali-soluble proteins into water-soluble and salt-soluble proteins. In addition, wheat malt endopeptidase did not break the disulfide bonds between proteins but affected the conformation of disulfide bonds between substrate protein molecules, which were changed from the gauche-gauche-trans (g-g-t) vibrational mode to the trans-gauche-trans (t-g-t) vibrational mode. Wheat malt endopeptidase exhibited the highest enzymatic activity at 2 h of enzymatic digestion, demonstrating the fastest hydrolytic rate of soybean meal proteins. Compared with the samples before enzymatic hydrolysis, the total alcohol- and alkali-soluble proteins were decreased by 11.89% but the water- and salt-soluble proteins were increased by 11.99%, indicating the hydrolytic effect of endopeptidase. The corresponding water-soluble proteins had molecular weights of 66.4–97.2, 29–44.3, and 20.1 kDa, while the salt-soluble proteins had molecular weights of 44.3–66.4, 29–44.3, and 20.1 kDa, respectively. The degree of enzymatic hydrolysis of soybean meal reached the maximum at 8 h. The newly created proteins exhibited significantly antioxidant properties, which were inversely related to the molecular weight. Proteins with molecular weight <3 kDa had the highest antioxidant performance with an antioxidant capacity of 1.72 ± 0.03 mM, hydroxyl radical scavenging rate of 98.04%, and ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] radical scavenging capacity of 0.44 ± 0.04 mM.
Survival rate of colorectal cancer in China: A systematic review and meta-analysis
Authors: Wang Ren , Lian Jie , Wang Xin , Pang Xiangyi , Xu Benjie , Tang Shuli , Shao Jiayue , Lu Haibo
Published: March 2023

Background

This study aims to comprehensively summarize the colorectal survival rate in China. Method: In PubMed and Web of Science, keywords such as “colorectal cancer”, “survival” and “China” were used to search literatures in the past 10 years. Random effect models were selected to summarize 1-year, 3-year, and 5-year survival rates, and meta-regression and subgroup analyses were performed on the included studies.

Results

A total of 16 retrospective and prospective studies providing survival rates for colorectal cancer in China were included. The 1-year, 3-year, and 5-year survival rates of colorectal cancer in China were 0.79, 0.72 and 0.62, respectively. In the included studies, the 5-year survival rates of stage I (5474 cases), stage II (9215 cases), stage III (8048 cases), and stage IV (4199 cases) colorectal cancer patients were 0.85, 0.81, 0.57 and 0.30, respectively. Among them, the 5-year survival rates of colorectal cancer were 0.82, 0.76, 0.71, 0.67, 0.66, 0.65 and 0.63 in Tianjin, Beijing, Guangdong, Shandong, Liaoning, Zhejiang and Shanghai, respectively.

Conclusion

The 5-year survival rate in China is close to that of most European countries, but still lower than Japan and South Korea, and the gap is gradually narrowing. Region, stage, differentiation, pathological type, and surgical approach can affect 5-year survival in colorectal cancer.

Systematic review registration

https://www.crd.york.ac.uk/prospero/ identifier, CRD42022357789.
Opportunities and challenges related to ferroptosis in glioma and neuroblastoma
Authors: Chi Huizhong , Li Boyan , Wang Qingtong , Gao Zijie , Feng Bowen , Xue Hao , Li Gang
Published: March 2023

A newly identified form of cell death known as ferroptosis is characterized by the peroxidation of lipids in response to iron. Rapid progress in research on ferroptosis in glioma and neuroblastoma has promoted the exploitation of ferroptosis in related therapy. This manuscript provides a review of the findings on ferroptosis-related therapy in glioblastoma and neuroblastoma and outlines the mechanisms involved in ferroptosis in glioma and neuroblastoma. We summarize some recent data on traditional drugs, natural compounds and nanomedicines used as ferroptosis inducers in glioma and neuroblastoma, as well as some bioinformatic analyses of genes involved in ferroptosis. Moreover, we summarize some data on the associations of ferroptosis with the tumor immunotherapy and TMZ drug resistance. Finally, we discuss future directions for ferroptosis research in glioma and neuroblastoma and currently unresolved issues.
Comparison of the efficacy and safety of fruquintinib and regorafenib in the treatment of metastatic colorectal cancer: A real-world study
Authors: Deng Ya-Ya , Zhang Xin-Yue , Zhu Peng-Fei , Lu Hong-Rui , Liu Qian , Pan Shuang-Yue , Chen Zhe-Ling , Yang Liu
Published: March 2023

Background

Fruquintinib and regorafenib have been approved for the third-line therapy of metastatic colorectal cancer (mCRC) in China. However, at present, there is a lack of head-to-head clinical trials on the comparison of efficacy and safety between the two drugs.

Materials and methods

The data of patients with mCRC who were treated with fruquintinib or regorafenib after the standard chemotherapy in Zhejiang Provincial People’s Hospital from October 2018 to November 2021 were collected and analyzed. The primary endpoints were overall survival (OS), progression-free survival (PFS) and adverse events. The secondary endpoints were the appropriate sequence, objective remission rate (ORR) and disease control rate (DCR) of fruquintinib and regorafenib.

Results

A total of 105 patients were enrolled in this study. The ORR of fruquintinib group (n=55) and regorafenib group (n=50) were 6.1% and 2.0%; the DCR were 65.3% and 54.2%, respectively. There was no significant difference in median OS (mOS) and PFS (mPFS) between the two groups (mOS:14.2 vs12.0 months, p=0.057; mPFS:4.4 vs 3.5 months, p=0.150). Combined immunotherapy showed a synergistic effect. The mPFS and mOS of fruquintinib combined with anti-PD-1 therapy were longer than those of fruquintinib monotherapy (mPFS:5.9 vs 3.0 months, p=0.009; mOS:17.5 vs 11.3 months, p=0.008). The mOS of patients treated with regorafenib combined with anti-PD-1 therapy was 14.8 months higher than that of regorafenib monotherapy (p=0.045). When combined with anti-PD-1 therapy, the mPFS and mOS of fruquintinib was significantly longer than regorafenib (mPFS:5.9 vs 3.8 months, p=0.018; mOS:17.5 vs 14.8 months, p=0.044). In the treatment sequence, the OS of patients treated with regorafenib and then fruquintinib was significantly longer than that of the reverse treatment sequence (15.0 vs 8.3 months, p=0.019). The adverse reactions were generally similar, but the incidence of hand-foot syndrome of regorafenib was higher than that of fruquintinib, while fruquintinib was more prone to grade 3 hypertension.

Conclusion

Fruquintinib monotherapy showed better disease control rate and objective remission rate in the post-line therapy of metastasis colorectal cancer. Notably, the combination of PD-1 immunotherapy brought the additional effect, especially in the fruquintinib combined with anti-PD-1 therapy. Patients treated with regorafenib and then fruquintinib was significantly longer than that of the reverse treatment sequence. The toxicity of fruquintinib and regorafenib are similar.
Comprehensive analysis of autophagy-related clusters and individual risk model for immunotherapy response prediction in gastric cancer
Authors: Yao Yanxin , Hu Xin , Ma Junfu , Wu Liuxing , Tian Ye , Chen Kexin , Liu Ben
Published: March 2023

Introduction

Autophagy can be triggered by oxidative stress and is a double-edged sword involved in the progression of multiple malignancies. However, the precise roles of autophagy on immune response in gastric cancer (GC) remain clarified.

Methods

We endeavor to explore the novel autophagy-related clusters and develop a multi-gene signature for predicting the prognosis and the response to immunotherapy in GC. A total of 1505 patients from eight GC cohorts were categorized into two subtypes using consensus clustering. We compare the differences between clusters by the multi-omics approach. Cox and LASSO regression models were used to construct the prognostic signature.

Results

Two distinct clusters were identified. Compared with cluster 2, the patients in cluster 1 have favorable survival outcomes and lower scores for epithelial-mesenchymal transition (EMT). The two subtypes are further characterized by high heterogeneity concerning immune cell infiltration, somatic mutation pattern, and pathway activity by gene set enrichment analysis (GSEA). We obtained 21 autophagy-related differential expression genes (DEGs), in which PTK6 amplification and BCL2/CDKN2A deletion were highly prevalent. The four-gene (PEA15, HSPB8, BNIP3, and GABARAPL1) risk signature was further constructed with good predictive performance and validated in 3 independent datasets including our local Tianjin cohort. The risk score was proved to be independent prognostic factor. A prognostic nomogram showed robust validity of GC survival. The risk score was significantly associated with immune cell infiltration status, tumor mutation burden (TMB), microsatellite instability (MSI), and immune checkpoint molecules. Furthermore, the model was efficient for predicting the response to tumor-targeted agent and immunotherapy and verified by the IMvigor210 cohort. This model is also capable of discriminating between low and high-risk patients receiving chemotherapy.

Conclusion

Altogether, our exploratory research on the landscape of autophagy-related patterns may shed light on individualized therapies and prognosis in GC.
Modulating the proliferative and cytotoxic properties of patient-derived TIL by a synthetic immune niche of immobilized CCL21 and ICAM1
Authors: Yunger Sharon , Geiger Benjamin , Friedman Nir , Besser Michal J. , Adutler-Lieber Shimrit
Published: March 2023

A major challenge in developing an effective adoptive cancer immunotherapy is the ex-vivo generation of tumor-reactive cells in sufficient numbers and with enhanced cytotoxic potential. It was recently demonstrated that culturing of activated murine CD8+ T-cells on a “Synthetic Immune Niche” (SIN), consisting of immobilized CCL21 and ICAM-1, enhances T-cell expansion, increases their cytotoxicity against cultured cancer cells and suppresses tumor growth in vivo. In the study reported here, we have tested the effect of the CCL21+ICAM1 SIN, on the expansion and cytotoxic phenotype of Tumor Infiltrating Lymphocytes (TIL) from melanoma patients, following activation with immobilized anti-CD3/CD28 stimulation, or commercial activation beads. The majority of TIL tested, displayed higher expansion when cultured on the coated SIN compared to cells incubated on uncoated substrate and a lower frequency of TIM-3+CD8+ cells after stimulation with anti-CD3/CD28 beads. Comparable enhancement of TIL proliferation was obtained by the CCL21+ICAM1 SIN, in a clinical setting that included a 14-day rapid expansion procedure (REP). Co-incubation of post-REP TIL with matching target cancerous cells demonstrated increased IFNγ secretion beyond baseline in most of the TIL cultures, as well as a significant increase in granzyme B levels following activation on SIN. The SIN did not significantly alter the relative frequency of CD8/CD4 populations, as well as the expression of CD28, CD25, several exhaustion markers and the differentiation status of the expanded cells. These results demonstrate the potential capacity of the CCL21+ICAM1 SIN to reinforce TIL-based immunotherapy for cancer patients.
Identification of the novel therapeutic targets and biomarkers associated of prostate cancer with cancer-associated fibroblasts (CAFs)
Authors: Zhai Xinyu , Chen Xinglin , Wan Zhong , Ge Minyao , Ding Yi , Gu Jianyi , Hua Jinjun , Guo Dongdong , Tan Mingyue , Xu Dongliang
Published: March 2023

Globally, prostate cancer remains a leading cause of mortality and morbidity despite advances in treatment. Research on prostate cancer has primarily focused on the malignant epithelium, but the tumor microenvironment has recently been recognized as an important factor in the progression of prostate cancer. Cancer-associated fibroblasts (CAFs) play an important role in prostate cancer progression among multiple cell types in the tumor microenvironment. In order to develop new treatments and identify predictive and prognostic biomarkers for CAFs, further research is needed to understand the mechanism of action of prostate cancer and CAF. In this work, we performed the single-cell RNA sequence analysis to obtain the biomarkers for CAFs, and ten genes were finally regarded as the marker genes for CAFs. Based on the ssGSEA algorithm, the prostate cancer cohort was divided into low- and high-CAFs groups. Further analysis revealed that the CAFs-score is associated with many immune-related cells and immune-related pathways. In addition, between the low- and high-CAFs tissues, a total of 127 hub genes were discovered, which is specific in CAFs. After constructing the prognostic prediction model, SLPI, VSIG2, CENPF, SLC7A1, SMC4, and ITPR2 were finally regarded as the key genes in the prognosis of patients with prostate cancer. Each patient was assigned with the risk score as follows: SLPI* 0.000584811158157081 + VSIG2 * -0.01190627068889 + CENPF * -0.317826812875334 + SLC7A1 * -0.0410213995358753 + SMC4 * 0.202544454923637 + ITPR2 * -0.0824652047622673 + TOP2A * 0.140312081524807 + OR51E2 * -0.00136602095885459. The GSVA revealed the biological features of CAFs, many cancer-related pathways, such as the adipocytokine signaling pathway, ERBB signaling pathway, GnRH signaling pathway, insulin signaling pathway, mTOR signaling pathway and PPAR signaling pathway are closely associated with CAFs. As a result of these observations, similar transcriptomics may be involved in the transition from normal fibroblasts to CAFs in adjacent tissues. As one of the biomarkers for CAFs, CENPF can promote the proliferation ability of prostate cancer cells. The overexpress of CENPF could promote the proliferation ability of prostate cancer cells. In conclusion, we discuss the potential prognostic and therapeutic value of CAF-dependent pathways in prostate cancer.
Clinical features and prognostic factors in patients diagnosed with lymphovascular invasion of testicular germ-cell tumors: Analysis based on the SEER database
Authors: Ke Hu , Jiang Shengming , He Ziqi , Song Qianlin , Yang Dashuai , Song Chao , Dong Caitao , Liu Junwei , Su Xiaozhe , Zhou Jiawei , Xiong Yunhe
Published: March 2023

Background

Lymphovascular invasion (LVI) is a high-risk factor for testicular germ-cell tumors (TGCT), but a prognostic model for TGCT-LVI patients is lacking. This study aimed to develop a nomogram for predicting the overall survival (OS) of TGCT-LVI patients.

Methods

A complete cohort of 3288 eligible TGCG-LVI patients (training cohort, 2300 cases; validation cohort, 988 cases) were obtained from the Surveillance, Epidemiology, and End Results database. Variables screened by multivariate Cox regression analysis were used to construct a nomogram, which was subsequently evaluated using the consistency index (C-index), time-dependent receiver operating characteristic curve (ROC), and calibration plots. The advantages and disadvantages of the American Joint Committee on Cancer (AJCC) staging system and the nomogram were assessed by integrated discrimination improvement (IDI) and net reclassification improvement (NRI). Decision-analysis curve (DCA) was used to measure the net clinical benefit of the nomogram versus the AJCC staging system. Finally, Kaplan–Meier curves were used to evaluate the ability to identify different risk groups between the traditional AJCC staging system and the new risk-stratification system built on the nomogram.

Results

Nine variables were screened by multivariate Cox regression analysis to construct the nomogram. The C-index (training cohort, 0.821; validation cohort, 0.819) and time-dependent ROC of 3-, 5-, and 9-year OS between the two cohorts suggested that the nomogram had good discriminatory ability. Calibration curves showed good consistency of the nomogram. The NRI values of 3-, 5-, and 9-year OS were 0.308, 0.274, and 0.295, respectively, and the corresponding values for the validation cohort were 0.093, 0.093, and 0.099, respectively (P<0.01). Additionally, the nomogram had more net clinical benefit as shown by the DCA curves, and the new risk-stratification system provided better differentiation than the AJCC staging system.

Conclusions

A prognostic nomogram and new risk-stratification system were developed and validated to assist clinicians in assessing TGCT-LVI patients.
Project Optimus, an FDA initiative: Considerations for cancer drug development internationally, from an academic perspective
Authors: Murphy Ravindhi , Halford Sarah , Symeonides Stefan Nicholas
Published: March 2023

Modern cancer therapeutics are increasingly targeted, bringing the promise of new and improved activity, alongside better tolerability. However, while many are indeed resulting in dramatic improvements in disease control and patient survival, short- and long-term tolerability has not always accompanied it. The choice of dose and schedule is often in the upper range of the therapeutic window, driven by the maximum tolerated dose (MTD) model of previous cytotoxic agents. There is increasing recognition that this needs to change, by taking a more holistic approach to determine the optimal dose for desired biological effects and tolerability early in clinical development. In the US, the FDA’s Oncology Centre of Excellence is addressing this via the Project Optimus initiative: aiming to reform dose optimisation studies so that they can demonstrate the most appropriate dose selection. Early clinical development will need to demonstrate the dose-exposure, -pharmacodynamic, -toxicity and -activity relationships, including randomised evaluations for dose selection. Regulatory agencies outside the US are similarly exploring this. Along with Australia, Brazil, Canada, Israel, Singapore and Switzerland, the UK participates in Project Orbis, a collaborative program with the FDA to accelerate patient access to new cancer medicines through coordinated regulatory review. Close alignment with Project Optimus will be important internationally and will require changes across industry, including for academic units and small biotech. We discuss our perspective on the implications, and opportunities, for early phase oncology trials as a uniquely charity-funded drug development facility, the Centre for Drug Development within the Cancer Research UK charity.
At-home dried blood spot (DBS) collection to increase population heterogeneity representation in pediatric research: An ECHO study
Authors: Green Joseph M. , Barry Fatoumata , Burton Phoebe , Beauchemin Jennifer , Huentelman Matthew J. , Deoni Sean C. L. , Lewis Candace R. ,   on behalf of program collaborators for Environmental Influences on Child Health Outcomes
Published: March 2023

Self-collection of dried blood samples (DBS) in the participant's home provides an alternative to university/hospital visits for research and has the potential to improve the representation of population heterogeneity in research. This study aimed to assess the feasibility of guardian and/or self-DBS collection in healthy youth in the lab and home. Guardians/youth [N = 140; females = 63; Mage = 8.73, SDage = 3.56] who enrolled in a longitudinal study of typical development were asked during a lab visit to provide a DBS. Upon providing a sample, the participants were asked if they would be willing to self-collect in the home and return the sample via the post office. Of those asked to provide a sample in the lab, 82% consented and 18% declined, with a significant difference in age but no significant difference in sex, ethnicity, race, or family income. Of those who provided a sample in the lab, 75% were willing to self-collect DBS in the home, with no significant difference in demographic variables between them. We report a quality assessment and DNA extraction results from a subset of samples. The results demonstrate a high feasibility of DBS collection from healthy youth for research purposes both in the laboratory and in the home across different demographic variables. Developmental researchers should consider including this approach in their studies to increase population heterogeneity representation.
The effects of early combined training on the physical development of preterm infants with different gestational ages
Authors: He Fang , Wu Na , Ma Xiuwei , Liu Xiaofang , Gao Ming , Feng Zhichun
Published: March 2023

Objective

To investigate the effects of early combined training on the physical development of preterm infants of different gestational ages.

Methods

A total of 144 preterm infants from our hospital's neonatal intensive care unit (NICU) between 2019 and 2020 were selected as the research participants and randomly divided into an intervention group (77 cases) and a control group (67 cases). The physical development and catch-up growth satisfaction rate of preterm infants in the intervention and control groups were compared at 40 weeks, 3 months, 6 months and 12 months of corrected age.

Results

At 40 weeks of gestational age and corrected 3 months of gestational age, the physical growth indexes of the intervention group were higher than those of the control group, with a statistical difference (P < 0.05). At the corrected age of 12 months, the body weight and length of preterm infants in the <29 weeks intervention group were still higher than those in the control group (P < 0.05). The body weight of preterm infants in the 29–32 weeks and 32–34 weeks intervention groups was higher than in the control group (P < 0.05). There was no statistical difference between the intervention and control groups in the 34–37 weeks category (P > 0.05). The catch-up growth satisfaction rates of all the physical growth indexes in the intervention group were higher than those of the control group at the corrected 3 months for all the gestational ages (P < 0.05). While those indexes in the three >29 weeks intervention groups were higher than those in the <29 weeks intervention group at the corrected age of 12 months (P < 0.05).

Conclusion

Early combined training can promote the early catch-up growth of preterm infants, especially in the early gestational age groups (<34 weeks), and the catch-up growth promotion can last for 12 months. The older the gestational age, the sooner catch-up growth satisfaction will occur.
Fontan-associated liver disease: Diagnosis, surveillance, and management
Authors: de Lange Charlotte , Möller Thomas , Hebelka Hanna
Published: March 2023

The Fontan operation is a lifesaving procedure for patients with functional single-ventricle congenital heart disease, where hypoplastic left heart syndrome is the most frequent anomaly. Hemodynamic changes following Fontan circulation creation are now increasingly recognized to cause multiorgan affection, where the development of a chronic liver disease, Fontan-associated liver disease (FALD), is one of the most important morbidities. Virtually, all patients with a Fontan circulation develop liver congestion, resulting in fibrosis and cirrhosis, and most patients experience childhood onset. FALD is a distinctive type of congestive hepatopathy, and its pathogenesis is thought to be a multifactorial process driven by increased nonpulsatile central venous pressure and decreased cardiac output, both of which are inherent in the Fontan circulation. In the advanced stage of liver injury, complications of portal hypertension often occur, and there is a risk of developing secondary liver cancer, reported at young age. However, FALD develops with few clinical symptoms, a surprisingly variable degree of severity in liver disease, and with little relation to poor cardiac function. The disease mechanisms and modifying factors of its development are still not fully understood. As one of the more important noncardiac complications of the Fontan circulation, FALD needs to be diagnosed in a timely manner with a structured monitoring scheme of disease development, early detection of malignancy, and determination of the optimal time point for transplantation. There is also a clear need for consensus on the best surveillance strategy for FALD. In this regard, imaging plays an important role together with clinical scoring systems, biochemical workups, and histology. Patients operated on with a Fontan circulation are generally followed up in cardiology units. Ultimately, the resulting multiorgan affection requires a multidisciplinary team of healthcare personnel to address the different organ complications. This article discusses the current concepts, diagnosis, and management of FALD, with special emphasis on the role of different imaging techniques in the diagnosis and monitoring of disease progression, as well as current recommendations for liver disease surveillance.
Identification and characterization of opportunistic pathogen Pectobacterium polonicum causing potato blackleg in China
Authors: Han Wanxin , Wang Jinhui , Pirhonen Minna , Pan Yang , Qin Jingxin , Zhang Shangqing , Zhu Jiehua , Yang Zhihui
Published: March 2023

Blackleg and aerial stem rot of potato (Solanum tuberosum L.), caused by soft rot enterobacteria of the genera Pectobacterium and Dickeya, has recently increased years in Hebei Province, China. Field surveys were performed during the 2021 potato growing season in Hebei to identify and characterize bacterial pathogens. Sixteen potato plants showing blackleg or aerial stem rot were collected from three potato-producing areas, and ten representative pectinolytic bacteria were isolated from symptomatic plants. 16S rDNA sequencing and multilocus sequence analysis were performed to determine the taxonomic position of the bacterial isolates. The isolates belonged to the genus Pectobacterium, including Pectobacterium atrosepticum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Pectobacterium parmentieri. The exceptions were isolates BY21311 and BY21312, which belonged to a new species of Pectobacterium polonicum previously found in groundwater. The taxonomy of isolate BY21311 was confirmed using whole genome-based analysis. P. polonicum has only been identified in potato plants on one farm in Baoding region in China. Isolates BY21311 and BY21312 displayed similar physiological and biochemical traits to the type strain DPMP315T. Artificial inoculation assays revealed that isolate BY21311 fulfilled Koch’s postulates for potato blackleg. These findings represent the first time P. polonicum, a water-associated Pectobacterium species may be the cause of blackleg in the field. Interestingly, P. polonicum BY21311 has reduced ability to macerate potato tubers when compared to P. atrosepticum, P. brasiliense, P. versatile, and P. parvum, which is more virulent in tubers than the type strain DPMP315T. The host range of isolate BY21311 was determined by injection method, which can impregnate five plants. Although the genome of isolate BY21311 harbors gene clusters encoding a type III secretion system, it did not elicit a hypersensitive response (HR) in Nicotiana benthamiana or N. tabacum leaves. T3SS effector AvrE and T4SS effector PilN were obtained by predicting isolate BY21311 genome. P. polonicum appears to show significant variations in gene content between two genomes, and gene content varies between isolates BY21311 and DPMP315T, with strain specific-genes involved in many aspects, including lipopolysaccharide biosynthesis, substrate translocation, T4SS and T6SS among others, suggesting that isolates BY21311 and DPMP315T might represent distinct clades within the species.
Strip intercropping with local crops increased Aconitum carmichaeli yield and soil quality
Authors: Liu Chen , Yan Pengdong , Liu Zhenyu , Zhang Jianglan , Zhang Guoyan , Cui Langjun
Published: March 2023

Aconitum carmichaeli Debx. is a traditional Chinese medicine that is cultivated in China and Japan. However, the monoculturing of this herb substantially decreases soil quality. Therefore, scientific planting management is crucial for resolving the current problems in the cultivation of A. carmichaeli. In this study, we conducted a comparative study on the soil environmental characteristics, herb growth and quality of A. carmichaeli intercropping with five local crops in two different areas. Herb growth and quality, including biomass and secondary metabolites, and rhizosphere soil environmental characteristics were measured. The results showed that the intercropping with the five local crops substantially improved the A. carmichaeli biomass and polysaccharide content, decreased the disease index, and altered three monoester diterpenoid alkaloids and three diester diterpenoid alkaloids accumulations. The intercrops also increased the soil pH, nitrogen-cycling-gene abundances, and potentially beneficial microorganism abundances, and it also changed the soil nutrient levels. Moreover, these intercropping patterns could alleviate the continuous cropping obstacles of A. carmichaeli. According to a comprehensive evaluation of the A. carmichaeli growth and quality, as well as the soil quality, the best intercropping systems were the A. carmichaeli intercropping with rice, maize, and peanut. In summary, the strip-intercropping systems could improve the A. carmichaeli growth and soil quality, and be beneficial to the sustainable ecological planting of A. carmichaeli.
Kidney transplant-related knowledge and influencing factors in Chinese kidney transplant candidates and recipients: A cross-sectional study
Authors: Ma Hangxia , Hu Maosen , Wan Jingjing
Published: March 2023

Objective

To investigate the kidney transplantation knowledge of kidney transplant (KT) candidates and recipients, and to explore the related influencing factors.

Methods

From March to July 2022, a total of 170 KT candidates and 270 KT recipients were investigated in two tertiary and Grade A hospitals in Hunan Province, China, using demographic questionnaires and the Kidney Transplant Understanding Tool (K-TUT). Multiple linear regression was used to explore the influencing factors of related knowledge of kidney transplantation.

Results

The scores of kidney transplantation knowledge of the two groups were 50.67 (Ranged: 0–63) and 52.79 (Ranged: 0–62), indicating a middle level of knowledge. Education level and whether they have received health education were significantly associated with the knowledge level of kidney transplantation in both KT candidates and recipients. In addition, age and fertility status were only significantly associated with the knowledge level of kidney transplantation in KT recipients.

Conclusion

Our finding shows that the knowledge level of KT candidates and recipients is not optimistic, which suggests that healthcare providers should pay more attention to the health education of this population. In addition, future health education interventions should consider the education level, age, and fertility status factors affecting kidney transplantation knowledge in KT candidates and recipients.
Acoustic information masking effects of natural sounds on traffic noise based on psychological health in open urban spaces
Authors: Zhang Shilun , Chen Lin
Published: March 2023

The use of existing resources, such as natural sounds, to promote the mental health of citizens is an area of research that is receiving increasing attention. This research contributes to existing knowledge by combining a field psychological walk method and an experimental acoustic control method to compare the acoustic information masking effects of water and birdsong sounds on traffic noise based on the psychological health responses of 30 participants to such effects. The influence of traffic noise and contextual sounds on the psychological health of participants identified the potential of natural sounds in the acoustic information masking of traffic noise. Furthermore, it was found that 65.0 dBA water sounds did not mask 60.0 dBA traffic noises. However, 45.0 dBA birdsong sounds did mask it, but this effect was not significant. Additionally, contextual factors with and without crowd activity sounds were not significant in influencing psychological health through birdsong. This study contributes to public health cost savings. It may also guide the development of new ideas and methods for configuring open urban spaces according to public health needs.
High-risk population and factors of stroke has changed among middle-aged and elderly Chinese—Evidence from 1989 to 2015
Authors: Zhang Xue , Dai Jing , Li Wei , Yang Yunjuan
Published: March 2023

Background

Stroke is an acute cerebrovascular disease with high mortality and disability. This study aimed to investigate the trend of stroke prevalence from 1989 to 2015 in China, explore the transition of high-risk population and high-risk factors, and provide some evidence to develop more targeted stroke intervention strategies.

Material and methods

We derived the baseline data from China Health and Nutrition Survey (CHNS). Participants responded to face-to-face interviews and examinations containing demographic information, behavioral health information, disease history, and physical examination. We applied chi-square test, shapley value decomposition model, and decision tree model to evaluate the changes of high-risk population and high-risk factors of stroke.

Results

Across 42,419 middle-aged and elderly residents, the prevalence of stroke was decreasing from 1989 to 2015. Hypertension was the leading risk factor of stroke, while its contribution rate was weakened with the increasing of medicine taking rate. As the second risk factor of stroke, the contribution of age decreased either. Meanwhile, the contribution rate of historical health factors, lifestyle factors, and regional factors, such as body mass index, diabetes, and living area to the impact of stroke was increasing. In addition, the first high-risk population of stroke changed from hypertension patients aged 75 years and above to without spouse residents living in stroke belt such as Beijing and Liaoning. The second risk population of stroke transformed from male hypertensive patients under 75 years old into male hypertensive patients living in urban. The third high-risk group turned from the elderly aged 75 and above into the female patients with hypertension and diabetes.

Conclusions

This study demonstrated that the high-risk population and high-risk factors of stroke changed in China and revealed the direction and internal mechanism of transition of stroke. Targeted stroke intervention strategies should be renewed. Health education for the high-risk population of stroke should be carried out, healthy living habits need be advocated, and the use of antihypertensive drugs for the hypertensive patients should be standardized.
Nutrition literacy differs based on demographics among University students in Bengbu, China
Authors: Gao Tianjing , Duan Ying , Qi Qi , Mo Guangju , Han Siyue , Liu Huaqing , Zhang Min
Published: March 2023

Background

Nutrition literacy (NL) encompasses the knowledge and skills that inform individuals' food choices. This cross-sectional study explored factors associated with NL among Chinese university students in Bengbu, China.

Methods

A cross-sectional survey was carried out. Two thousand one hundred thirty-three university students were selected by stratified cluster sampling. A 43-item NL questionnaire was used to assess NL. Binary logistic regression was used to determine odds ratios (ORs) along with 95% confidence intervals (CIs) for NL and to test the interaction effects of multiple factors on total NL and its six dimensions.

Results

Of these participants, 1,399 (65.6%) were women and 734 (34.4%) were men. Students who were from urban areas (OR = 1.36, 95% CI: 1.08–1.72), were living with both parents (OR = 1.30, 95% CI: 1.02–1.65), and had high academic performance (OR = 1.85, 95% CI: 1.34–2.57) were more likely to report higher NL levels than did other students. The ORs for NL (OR = 1.60, 95% CI: 1.06–2.41), nutrition knowledge (OR = 1.51, 95% CI: 1.00–2.26), obtaining skills (OR = 1.76, 95% CI: 1.16–2.65), and critical skills (OR = 1.59, 95% CI: 1.05–2.39) were higher for medical students who had received nutrition education than for other students. The ORs for NL (OR = 2.42, 95% CI: 1.21–4.84), nutrition understanding (OR = 2.59, 95% CI: 1.28–5.25), and interactive skills (OR = 2.06, 95% CI: 1.04–4.08) were higher for only-child students and those with a monthly expenditure of >¥1500.

Conclusions

NL of university students differed in terms of place of origin, living arrangement, nutrition education, academic performance, and household income, and the findings imply that universities should have all students take a basic nutrition course to improve their NL.
An anonymization-based privacy-preserving data collection protocol for digital health data
Authors: Andrew J. , Eunice R. Jennifer , Karthikeyan J.
Published: March 2023

Digital health data collection is vital for healthcare and medical research. But it contains sensitive information about patients, which makes it challenging. To collect health data without privacy breaches, it must be secured between the data owner and the collector. Existing data collection research studies have too stringent assumptions such as using a third-party anonymizer or a private channel amid the data owner and the collector. These studies are more susceptible to privacy attacks due to third-party involvement, which makes them less applicable for privacy-preserving healthcare data collection. This article proposes a novel privacy-preserving data collection protocol that anonymizes healthcare data without using a third-party anonymizer or a private channel for data transmission. A clustering-based k-anonymity model was adopted to efficiently prevent identity disclosure attacks, and the communication between the data owner and the collector is restricted to some elected representatives of each equivalent group of data owners. We also identified a privacy attack, known as “leader collusion”, in which the elected representatives may collaborate to violate an individual's privacy. We propose solutions for such collisions and sensitive attribute protection. A greedy heuristic method is devised to efficiently handle the data owners who join or depart the anonymization process dynamically. Furthermore, we present the potential privacy attacks on the proposed protocol and theoretical analysis. Extensive experiments are conducted in real-world datasets, and the results suggest that our solution outperforms the state-of-the-art techniques in terms of privacy protection and computational complexity.
Epidemiological status and associated factors of frailty and pre-frailty in older adults with asthma in China: A national cross-sectional study
Authors: Zeng Xue-zhai , Meng Ling-bing , Jia Na , Shi Jing , Zhang Chi , Li Ying-ying , Hu Xing , Hu Jia-bin , Li Jian-yi , Wu Di-shan , Li Hui , Qi Xin , Wang Hua , Zhang Qiu-xia , Li Juan , Liu De-ping
Published: March 2023

Objective

There are few studies on the prevalence and factors associated with frailty and pre-frailty in older adults with asthma worldwide. The aim of this study was to examine the epidemiological status and factors associated with frailty and pre-frailty in older adults with asthma in China.

Research design and methods

Data were obtained from the Sample Survey of Aged Population in Urban and Rural China in 2015, a nationwide cross-sectional survey covering 224,142 older people aged 60 years or older in 31 provinces/autonomous regions/municipalities in mainland China. We performed frailty and pre-frailty assessments using the frailty index, and the diagnosis of asthma in the older adults was self-reported based on the history of the physician's diagnosis.

Results

Nine thousand four hundred sixteen older adults with asthma were included in the study. The age-sex standardized prevalence of frailty and pre-frailty in Chinese older adults with asthma was 35.8% (95% CI 34.8%−36.7%) and 54.5% (95% CI 53.5%−55.5%). Multinomial logistic regression analysis showed that increased age, female, illiteracy, living alone, poor economic status, ADL disability, comorbid chronic diseases, previous hospitalization in the past year, and residence in northern China were associated with frailty and pre-frailty in older adults with asthma.

Conclusion

The prevalence of frailty and pre-frailty in Chinese older adults with asthma is very high, and assessment of frailty should become routine in the management of older adults with asthma. Appropriate public health prevention strategies based on identified risk factors for frailty in older adults with asthma should be developed to reduce the burden of frailty in Chinese older adults with asthma.
Utilization of three-layers heterogeneous mammographic phantom through MCNPX code for breast and chest radiation dose levels at different diagnostic X-ray energies: A Monte Carlo simulation study
Authors: ALMisned Ghada , Elshami Wiam , Kilic G. , Rabaa Elaf , Zakaly Hesham M. H. , Ene Antoaneta , Tekin H. O.
Published: March 2023

Introduction

We report the breast and chest radiation dose assessment for mammographic examinations using a three-layer heterogeneous breast phantom through the MCNPX Monte Carlo code.

Methods

A three-layer heterogeneous phantom along with compression plates and X-ray source are modeled. The validation of the simulation code is obtained using the data of AAPM TG-195 report. Deposited energy amount as a function of increasing source energy is calculated over a wide energy range. The behavioral changes in X-ray absorption as well as transmission are examined using the F6 Tally Mesh extension of MCNPX code. Moreover, deposited energy amount is calculated for modeled body phantom in the same energy range.

Results and discussions

The diverse distribution of glands has a significant impact on the quantity of energy received by the various breast layers. In layers with a low glandular ratio, low-energy primary X-ray penetrability is highest. In response to an increase in energy, the absorption in layers with a low glandular ratio decreased. This results in the X-rays releasing their energy in the bottom layers. Additionally, the increase in energy increases the quantity of energy absorbed by the tissues around the breast.
The interplay between the microbiome and colonic immune system in checkpoint inhibitor therapy
Authors: Dehinsilu Jacob , Sergaki Chrysi , Amos Gregory , Fontana Vanessa , Pirmohamed Munir
Published: March 2023

The advent of immune checkpoint inhibitor therapy was a significant step in the development of treatments for cancer. It is, however, a double-edged sword. Immune related adverse events are the result of unleashing brakes on the immune system and affect many patients undergoing checkpoint inhibitor therapy, often being debilitating and occasionally lethal. It has been shown both in mice and in humans that the presence of certain families, genera and species of bacteria are associated with improved responses to checkpoint inhibitor therapy, whereas in their absence the response to therapy is often poor. Recent studies have demonstrated that immune related adverse events to checkpoint inhibitor therapy can be perturbed and perhaps predicted based on the composition and functional capacity of the gut microbiota and parts of the immune system. In the case of colitis associated with immune checkpoint inhibitor therapy, one interesting avenue of investigation is based on the activity of secretory immunoglobulin A (SIgA). Produced by plasma cells, IgA is present in high concentrations at the gut mucosa and is involved in both the maturation and maintenance of the microbiota as well as the development of IBD. Here we summarise the current literature surrounding the interplay between the gut microbiota and response to CPI therapy. Additionally, we overview the colonic immune system, paying particular attention to IgA, as a key component of the microbiota-immune system interaction.
Emerging trend and pattern of urbanization and its contribution from migration in Gujarat: Evidence from district level analysis
Authors: Roy Anil Kumar , Saha Meghnad
Published: March 2023

Gujarat has been one of the leading states in India as far as the levels of urbanization are concerned. This is due to its pull factors attracting migrations from outside the state. However, the urban population is concentrated in a few districts dominated by large urban centers such as Ahmedabad, Surat, Rajkot, Vadodara, Bhavnagar and Jamnagar. The levels of urbanization in Gujarat have been higher than the national average throughout independent India. It has also experienced rapid urban growth of population during the same period, but the recently emerging concentration and pattern of urbanization have been lopsided, meaning that a few districts contributes more than half of the total urban population of the state. Literature in the thematic areas of urbanization particularly in Gujarat are few and limited. The studies so far have discussed about the impact of globalization and urbanizations, trends and patters of urbanization, dynamics of urban development and migration in Gujarat. This study particularly focuses on establishing a link between migration and urbanization using district level data on urbanization. This study is unique in the sense that previous studies have failed to capture the interstate migration and levels of urbanization in the state of Gujarat in India. Recent census data show a substantial increase in the census towns in Gujarat in the vicinity of the developed corridor and within the influence areas of large metropolitan cities. This paper aims to analyze the trends and emerging patterns in the levels of urbanization at the district level. It attempts to understand the inequalities in the distribution of urban population using the GINI coefficient of different size-class towns during the 2001 and 2011 censuses. It also tries to map the distribution of the urban population at the district level to show the emerging pattern. Emerging trends and the existing network of the census towns of 2011 have also been mapped to provide the idea of regional distribution. The existing urbanization pattern in Gujarat matches with the industrial development. It reminds colonial experiences of induced industrialization, attracting colossal in-migration and higher urbanization. The migration pattern during the last two census periods shows that respective districts of the above-mentioned cities are attracting in-migration due to the early post-independence industrialization period. We have calculated the net contribution from migration to the levels of urbanization using D-2 Series migration data from the census of India for the years 2001 and 2011. It shows that Central Gujarat, Saurashtra and South Gujarat have gained more due to migrations from other states to sustain their industrial development, leading to rapid and higher levels of urbanization in these regions. However, the future of urbanization in the state will depend on carefully selecting industrial activities that are sustainable in the long run.