bluuebunny/crossref_metadata_2025 huggingface dataset:
Ellenberg Indicator Values Disclose Complex Environmental Filtering Processes in Plant Communities along an Elevational Gradient
Authors: Di Biase Letizia , Tsafack Noelline , Pace Loretta , Fattorini Simone
Published: January 2023

Ellenberg indicator values (EIVs) express plant preferences for temperature, light, continentality, soil moisture, pH, and soil nutrients, and have been largely used to deduce environmental characteristics from plant communities. However, EIVs might also be used to investigate the importance of filtering mechanisms in shaping plant communities according to species ecological preferences, a so far overlooked use of EIVs. In this paper, we investigated how community-weighted means (CWM), calculated with EIVs, varied along an elevational gradient in a small mountain in Central Italy. We also tested if species abundances varied according to their ecological preferences. We found that the prevalence of thermophilous species declines with elevation, being progressively replaced by cold-adapted species. Heliophilous species prevail at low and high elevations (characterized by the presence of open habitats), whereas in the middle of the gradient (occupied by the beech forest), sciophilous species predominate. Variations for moisture and soil nutrient preferences followed a similar pattern, probably because of the high moisture and nutrient levels of forest soils with a lot of humus. No distinct pattern was detected for EIVs for pH and continentality since these factors are subject to more local variations. These results highlight the possible role of EIVs to investigate how environmental gradients shape plant communities.
Micropropagation Protocol and Genetic Stability of the Salix myrtilloides Plants Cultivated In Vitro
Authors: Parzymies Marzena , Pogorzelec Magdalena , Głębocka Katarzyna , Sliwinska Elwira
Published: January 2023

Salix myrtilloides L. is a relict species, threatened with extinction in many European countries. To prevent the loss of the species, tissue culture was established to produce plant material for reintroduction in natural habitats. Micropropagation was chosen as a method to obtain new plants. S. myrtilloides shoots were disinfected with NaOCl, AgNO3, or with a two-step disinfection with NaOCl, and then placed on MS medium supplemented with BA at 1 mg·dm−3 and IBA at 0.1 mg·dm−3. Regenerated shoots were cultivated in presence of BA, KIN, and 2iP to select the treatment with the highest multiplication rate. The obtained plants were acclimatized to ex vitro conditions. Inter-simple sequence repeat (ISSR) and flow cytometric analyses were conducted on in vitro regenerated plants to check their genetic stability. The best disinfection results were obtained when explants were treated with 1.5% NaOCl for 20 min. The highest multiplication rate and good quality plants were noted in the control media, without growth regualtors and in presence of kinetin at 0.5 mg·dm−3. Flow cytometry and ISSR analyses confirmed genetic stability in plantlets, which indicated the possibility to use the in vitro obtained plants for reintroduction.
Applications of Mass Spectrometry in Dentistry
Authors: Kallianta Meletia , Pappa Eftychia , Vastardis Heleni , Rahiotis Christos
Published: January 2023

Mass Spectrometry (MS) is one of the fastest-developing methods in analytical instrumentation. As a highly sensitive, universal detector, it can identify known and unknown compounds, which can indeed be found in a minimal concentration. This review aims to highlight the significant milestones in MS applications in dentistry during recent decades. MS can be applied in three different fields of dentistry: (1) in research of dental materials and chemical agents, (2) in laboratory analysis of biospecimens, and (3) as a real-time diagnostic tool in service of oral surgery and pathology. MS applications on materials and agents may focus on numerous aspects, such as their clinical behavior, possible toxicity, or antimicrobial properties. MS is also a valuable, non-invasive tool for biomarkers’ detection in saliva and has found great application in -omics technologies as it achieves efficient structure-finding in metabolites. As metabolites are located beyond the central dogma, this technique can provide a complete understanding of cellular functions. Thus, it is possible to determine the biological profile in normal and pathological conditions, detect various oral or systematic diseases and conditions, and predict their course. Lastly, some promising advances concerning the surgical approach to potentially oral malignant or malignant disorders exist. This breakthrough method provides a comprehensive approach to dental materials research and biomarker discovery in dental and craniofacial tissues. The current availability of various ‘OMIC’ approaches paves the way for individualized dentistry and provides suggestions for clinical applications in the point-of-care hubs.
The Effectiveness and Toxicity of Frameless CyberKnife Based Radiosurgery for Parkinson’s Disease—Phase II Study
Authors: Goc Bartłomiej , Roch-Zniszczoł Agata , Larysz Dawid , Zarudzki Łukasz , Stąpór-Fudzińska Małgorzata , Rożek Agnieszka , Woźniak Grzegorz , Boczarska-Jedynak Magdalena , Miszczyk Leszek , Napieralska Aleksandra
Published: January 2023

Frame-based stereotactic radiosurgery (SRS) has an established role in the treatment of tremor in patients with Parkinson’s disease (PD). The low numbers of studies of frameless approaches led to our prospective phase 2 open-label single-arm clinical trial (NCT02406105), which aimed to evaluate the safety and efficacy of CyberKnife frameless SRS. Twenty-three PD patients were irradiated on the area of the thalamic ventral nuclei complex with gradually increasing doses of 70 to 105 Gy delivered in a single fraction. After SRS, patients were monitored for tremor severity and the toxicity of the treatment. Both subjective improvement and dose-dependent efficacy were analysed using standard statistical tests. The median follow-up was 23 months, and one patient died after COVID-19 infection. Another two patients were lost from follow-up. Hyper-response resulting in vascular toxicity and neurologic complications was observed in two patients irradiated with doses of 95 and 100 Gy, respectively. A reduction in tremor severity was observed in fifteen patients, and six experienced stagnation. A constant response during the whole follow-up was observed in 67% patients. A longer median response time was achieved in patients irradiated with doses equal to or less than 85 Gy. Only two patients declared no improvement after SRS. The efficacy of frameless SRS is high and could improve tremor control in a majority of patients. The complication rate is low, especially when doses below 90 Gy are applied. Frameless SRS could be offered as an alternative for patients ineligible for deep brain stimulation; however, studies regarding optimal dose are required.
Lethal Lust: Suicidal Behavior and Chemsex—A Narrative Review of the Literature
Authors: Strasser Martina , Halms Theresa , Rüther Tobias , Hasan Alkomiet , Gertzen Marcus
Published: January 2023

Chemsex is described as the use of certain drugs—commonly methamphetamine, gamma-butyrolactone (GBL)/gammahydroxybutyrate (GHB), and mephedrone—before or during planned sexual activity primarily among men who have sex with men (MSM). Evidence shows that MSM who engage in chemsex are at increased risk of physical harm, such as sexually transmittable infections (STIs), and are more likely to experience mental health symptoms. To further assess this, we reviewed the recent literature to evaluate whether the psychological impact of chemsex behavior includes suicidal ideation and suicidal attempts. Pubmed/MEDLINE was searched for articles reporting suicidal ideation and behavior among chemsex users with the terms “chemsex”, “sexualized drug use”, “suicide”, and “mental health”. Twelve articles (three case reports and nine cross-sectional studies) were included in the final narrative review. Overall, we retrieved mixed results regarding the relationship between chemsex practice and suicidality outcomes. Considering the inhomogeneous nature of the studies, the findings indicate that suicidality could be an issue of concern among MSM in general but among chemsex users in particular. Possible risk factors for suicidality among chemsex participants may include adversities experienced due to one’s sexual orientation and an increased risk for HIV and other STI infections and the resulting negative impact on mental well-being. These aspects warrant further investigations.
Pavement Performance and Ice-Melting Characteristics of Asphalt Mixtures Incorporating Slow-Release Deicing Agent
Authors: Zhang Jiaqiang , Wang Weicheng , Liu Jinzhou , Wang Shuyi , Qin Xiaochun , Yu Bin
Published: January 2023

Icy pavement is one of the primary causes affecting driving safety in winter, and deicing asphalt mixture could effectively resist pavement icing. This study evaluated the effect of a slow-release deicing agent on pavement performance and ice-melting characteristics of the asphalt mixture. The asphalt mixture containing four different contents (0%, 30%, 50%, and 70% using the internal mixing method) by replacing mineral filler was designed. Pavement performance tests were used to investigate the effect of the deicing agent on the high-temperature stability, cracking resistance, and water stability of the asphalt mixture. Qualitative and quantitative tests were designed to compare the ice-melting characteristics and predict the ice-melting durability with different replacement amounts. The experimental results show that the high-temperature stability, low-temperature cracking resistance, and water stability of the asphalt mixture decrease with the increase inthe deicing agent. The mineral filler with a content of 50% deicing agent will enhance the stability of the mixture in the short term. Deicing asphalt mixture can significantly improve the ice and snow melting ability of the pavement, and the asphalt mixture with a content of 50% deicing agent will reduce the interface adhesion between mixture and ice by more than 55%. The slow-release deicing asphalt mixture can reach the maximum release concentration within two hours under rain and snow. The recommended replacement amount of slow-release deicing agent is 50%, and the predicted durability of deicing asphalt mixture is 5–8 years.
Influence of Organizational Culture on Construction Firms’ Performance: The Mediating Roles of Innovation and Marketing Capabilities
Authors: Osman Abdul Manaan , Liu Yisheng , Wang Zhaojing
Published: January 2023

Local Ghanaian construction firms have been accused of underperformance due to inadequate resources and capabilities, lack of market information, poor managerial skills, and other external factors. While construction firms may be unable to control external challenges, how they mobilize internal resources to confront them may be crucial for their performance and survival. An emerging consensus is that how organizations use their resources to respond to externalities is related to their organizational culture (OC). We contend that the unique contribution of organizational culture to firm performance (FP) may diminish in the absence of appropriate firm capabilities. Drawing on the resource-based view and capability theories, we attempt to investigate (a) how the types of OC influence FP and (b) whether innovation and marketing capabilities mediate the link between types of OC and construction firm performance. Results of partial least squares structural equation modelling show that clan, adhocracy, and market culture have significant positive association with FP, while hierarchy culture is negatively related to FP. Furthermore, innovation and marketing capabilities demonstrate a combination of full and partial mediation effects on the link between OC types and FP. Thus, the results direct senior managers’ attention towards the significance of soft assets in construction firm performance outcomes, signaling a shift from a purely technical and rationalistic approach to a more adaptable and humanistic approach. It implies that while emphasizing cultural values, managers should also give priority to these two capability dimensions.
Synthesis and Characterization of ZnO Doped TiO2 Nanocomposites for Their Potential Photocatalytic and Antimicrobial Applications
Authors: Gunasekaran Anguraj , Rajamani Ashok Kumar , Masilamani Chandrasekar , Chinnappan Inmozhi , Ramamoorthy Uthrakumar , Kaviyarasu Kasinathan
Published: January 2023

As a result of the sol-gel method, we were able to produce pure ZnO and ZnO-doped TiO2 nanocomposites. The hexagonal wurtzite phase in ZnO products was discovered by powder X-ray diffraction (XRD). ZnO products are typically hexagonal wurtzite crystallites, formed according to the Debye Scherrer formula. Nanocomposites with significant morphological changes were created using the sol-gel process, including those that resembled rocks. To determine the composition of Zn, O, and Ti atoms in the samples, a multidimensional X-ray analysis was performed. There is an energy gap between 3.61 eV, as determined by UV-vis spectroscopy. In this study, pure ZnO and ZnO-doped TiO2 nanocomposites were used to study the degradation of methylene blue (MB) under visible light irradiation. Over an irradiation course of 6 h, a ZnO-doped TiO2 composite (84%) were studied. As determined by the kinetic analysis, nanocomposites made from pure ZnO and ZnO-doped TiO2 followed pseudo-first-order kinetics. In the presence of ZnO-doped TiO2 nanocomposites, antibacterial activity was significantly improved. This was shown to be effective against Gram-positive and Gram-negative bacteria (Escherichia coli and B. sublittus). There is evidence that the metal oxide nanocomposites that are produced can be used as an appropriate antimicrobial and disinfection alternative, particularly in biomedical settings, as reported in more detail.
A Critical Review of Photo-Based Advanced Oxidation Processes to Pharmaceutical Degradation
Authors: Gonzaga Isabelle M. D. , Almeida Caio V. S. , Mascaro Lucia H.
Published: January 2023

Currently, the production and consumption of pharmaceuticals is growing exponentially, making them emerging contaminants that cause hazards to the ecological environment and human health. These drugs have been detected in surface water and drinking water around the world. This indicates that the conventional treatments used are ineffective for the removal of these compounds from the water, since they are very complex, with high stability and have high persistence in aquatic environments. Considering this problem, several types of alternative treatments, such as advanced oxidative processes, have been studied. Of these, AOPs using irradiation have received increasing interest due to their fast reaction rate and the ability to generate oxidizing species, which leads to an efficient degradation and mineralization of organic compounds, thus improving the quality of water and allowing its reuse. Therefore, in this review, we focus on the advances made in the last five years of irradiated AOPs in the degradation of different classes of pharmaceutical compounds. The articles address different study parameters, such as the method of the synthesis of materials, oxidants used, treatment time, type of light used and toxicity of effluents. This review highlights the success of irradiated AOPs in the removal of pharmaceuticals and hopes to help the readers to better understand these processes and their limitations for removing drugs from the environment. It also sheds light on some paths that future research must follow so that the technology can be fully applied.
Acknowledgment to the Reviewers of Challenges in 2022
Authors:   Challenges Editorial Office
Published: January 2023

High-quality academic publishing is built on rigorous peer review [...]
Indications and Timing of Guided Growth Techniques for Pediatric Upper Extremity Deformities: A Literature Review
Authors: Siemensma Mark F. , van Bergen Christiaan J.A. , van Es Eline M. , Colaris Joost W. , Eygendaal Denise
Published: January 2023

Osseous deformities in children arise due to progressive angular growth or complete physeal arrest. Clinical and radiological alignment measurements help to provide an impression of the deformity, which can be corrected using guided growth techniques. However, little is known about timing and techniques for the upper extremity. Treatment options for deformity correction include monitoring of the deformity, (hemi-)epiphysiodesis, physeal bar resection, and correction osteotomy. Treatment is dependent on the extent and location of the deformity, physeal involvement, presence of a physeal bar, patient age, and predicted length inequality at skeletal maturity. An accurate estimation of the projected limb or bone length inequality is crucial for optimal timing of the intervention. The Paley multiplier method remains the most accurate and simple method for calculating limb growth. While the multiplier method is accurate for calculating growth prior to the growth spurt, measuring peak height velocity (PHV) is superior to chronological age after the onset of the growth spurt. PHV is closely related to skeletal age in children. The Sauvegrain method of skeletal age assessment using elbow radiographs is possibly a simpler and more reliable method than the method by Greulich and Pyle using hand radiographs. PHV-derived multipliers need to be developed for the Sauvegrain method for a more accurate calculation of limb growth during the growth spurt. This paper provides a review of the current literature on the clinical and radiological evaluation of normal upper extremity alignment and aims to provide state-of-the-art directions on deformity evaluation, treatment options, and optimal timing of these options during growth.
Home Immunization with Palivizumab-A Randomized Pilot Study Describing Safety Aspects and Parents’ Preferences
Authors: Ebersjö Christina , Berggren Broström Eva , Kull Inger , Lindholm Olinder Anna
Published: January 2023

Among prematurely born infants and newborns with chronic conditions, a respiratory syncytial virus (RSV) infection may cause (re-)admission and later respiratory complications. Therapeutic protection is possible with monthly injections of a specific monoclonal antibody, palivizumab, during RSV season. Standard care is giving up to five injections in clinic-based settings. Immunization at home could be an alternative to standard care for vulnerable infants to reduce the number of revisits and associated risk of RSV infection. The aim of this randomized pilot trial was to evaluate safety aspects and explore parents’ preferences of home versus hospital immunization with palivizumab during one RSV season. Immediate adverse events (AEs) were observed and registered by a pediatric specialist nurse. Late-onset AEs were reported by parents. Parents’ perceptions were collected through a questionnaire and analyzed using content analysis. The study population consisted of 43 infants in 38 families. No immediate AEs occurred. Three late-onset AEs were reported in two infants in the intervention group. Three categories emerged in the content analysis: (1) protect and watch over the infant, (2) optimal health and well-being for the whole family, and (3) avoid suffering for the infant. The study results show that home immunization with palivizumab is feasible if safety aspects are considered and that parental involvement in the choice of place for immunization after a neonatal intensive care experience can be important.
Validation of a New Soccer Shooting Test Based on Speed Radar Measurement and Shooting Accuracy
Authors: Engler Felix , Hohmann Andreas , Siener Maximilian
Published: January 2023

Due to poor results, the German talent identification and development of the German soccer association DFB no longer performs a shooting test since a few years. The aim of this study was to create and validate a new soccer shooting test that allows valid conclusions to be drawn from the shooting quality of youth soccer players about their overall soccer skills. The shooting test was performed with a total of 57 male club players (age: 15.24 ± 0.864 years) from four different teams from the first, second, fifth, and the seventh division of the respective age group (under 15-year-olds until under 17-year-olds). Each subject took one shot at maximum shot speed and eight target shots, measuring accuracy and the shot speed. A multivariable linear regression analysis with forward selection revealed significant values for the variables average shot speed nondominant leg (p < 0.001) and total score (p = 0.004; accuracy × speed of every target shot). Based on these two variables, the soccer skills could be derived from the shooting skills of the adolescents in 57.4% of the cases. The study shows the importance of a good technique with the nondominant leg and of the ability to shoot accurately as well as fast simultaneously.
Effects of Musical Classes on Motor Creativity According to Age, Sex, and Weight Status in Young Students: A Music-Oriented versus Conventional Education Plan
Authors: Trecroci Athos , Signorini Gabriele , Scurati Raffaele , Colella Dario , Raiola Gaetano , Rigon Marta , Invernizzi Pietro Luigi
Published: January 2023

Motor creativity can be influenced by the specificity of the school–class environments (music-based education plans) and individual characteristics. This study aimed to investigate the effects of music oriented and conventional education plans on rhythmic perceptive capacity, motor creativity, and skill- and health-related fitness components in young students according to age, sex, and weight status. One hundred sixty-three young Italian students from elementary (second and fourth grade) and middle school (sixth and eighth grade) were enrolled in the study according to their education plan (music oriented or conventional). All participants were tested for rhythmic perceptive capacity (Stambak’s test), motor creativity (Divergent Movement Ability test), skill-related (Körperkoordinationstest Für Kinder), and health-related (Multistage Fitness test) components. Individuals were also considered according to age (elementary and middle school), sex, and weight status. Significant age × education plan and sex × education plan interactions (p < 0.01) were found in motor creativity (locomotor and stability skills) and motor competence (balance and jumping-like activities). No significant weight status × education plan interaction was found. The predominant role of music in the music-oriented education plan appeared to foster the ability to enhance motor creativity in elementary and middle school students compared to the conventional plan. Moreover, music-oriented experience also seems relevant for expressing and exhibiting motor competence (i.e., balance) in relation to sex.
Fabrication of Selective and Sensitive Hydrazine Sensor Using Sol-Gel Synthesized MoSe2 as Efficient Electrode Modifier
Authors: Alsalme Ali , Alsaeedi Huda
Published: January 2023

Due to its hazardous nature, the determination of hydrazine is of great significance. This study designed and fabricated a hydrazine electrochemical sensor. Two-dimensional (2-D) molybdenum diselenide (MoSe2) has been synthesized by using the sol-gel method. The phase purity and formation of MoSe2 was determined by a powder X-ray diffractometer. The surface morphological characteristics of the MoSe2 were studied by scanning electron microscopy. The presence of Mo and Se elements in the synthesized MoSe2 was checked by energy dispersive X-ray spectroscopy. The glassy carbon (GC) electrode (3 mm) was modified with the prepared MoSe2 via a drop-cast approach. This MoSe2-glassy carbon (MoSe2-GC) electrode was used as the working electrode for the hydrazine sensing application. The electrochemical sensing properties of the fabricated MoSe2-GC were analyzed by linear sweep voltammetry and cyclic voltammetry. The MoSe2-GC has shown a sensitivity of 0.68 µA/µMcm2 and a detection limit of 0.091 µM. In addition, MoSe2-GC also has good selectivity toward hydrazine determination in the presence of various interfering compounds. The excellent electro-catalytic behavior of MoSe2 is solely responsible for this enhanced sensing performance of MoSe2-GC.
Crystallographic Characterization of Sodium Ions in a Bacterial Leucine/Sodium Symporter
Authors: Karasawa Akira , Liu Haijiao , Quick Matthias , Hendrickson Wayne A. , Liu Qun
Published: January 2023

Na+ is the most abundant ion in living organisms and plays essential roles in regulating nutrient uptake, muscle contraction, and neurotransmission. The identification of Na+ in protein structures is crucial for gaining a deeper understanding of protein function in a physiological context. LeuT, a bacterial homolog of the neurotransmitter:sodium symporter family, uses the Na+ gradient to power the uptake of amino acids into cells and has been used as a paradigm for the study of Na+-dependent transport systems. We have devised a low-energy multi-crystal approach for characterizing low-Z (Z ≤ 20) anomalous scattering ions such as Na+, Mg2+, K+, and Ca2+ by combining Bijvoet-difference Fourier syntheses for ion detection and f” refinements for ion speciation. Using the approach, we experimentally identify two Na+ bound near the central leucine binding site in LeuT. Using LeuT microcrystals, we also demonstrate that Na+ may be depleted to study conformational changes in the LeuT transport cycle.
Prognostic Significance of p53 and p63 in Diffuse Large B-Cell Lymphoma: A Single-Institution Experience
Authors: Moreno Juan , Bahmad Hisham , Aljamal Abed , Delgado Ruben , Salami Ali , Guillot Carolina , Castellano-Sánchez Amilcar , Medina Ana , Sriganeshan Vathany
Published: January 2023

Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoma in adults. We evaluated the immunohistochemical (IHC) expression of p63 and p53 in DLBCL and their significance on overall survival (OS) and progression-free survival (PFS). We conducted a retrospective cohort study of 177 patients with DLBCL who presented to Mount Sinai Medical Center of Florida (Miami Beach, Florida) between 2010 and 2020. IHC staining for p63 and p53 protein expression was performed. A significant correlation was found between p63 positivity and p53 expression, p53/p63 co-positivity, Ki-67 proliferation index, MYC expression, and MYC/BCL2 double expression. Regardless of the germinal center B-cell like (GCB) subgrouping, there was a trend among p53+ patients to have MYC/BCL2 double expression, positive MYC expression, and lower OS and PFS. A tendency of poor OS was seen in p53+ patients in the non-GCB, GCB, and double expressors subgroups and poor PFS in p53+ patients regardless of the subgrouping. In conclusion, our results suggest that p63 and p53 may represent potential additional prognostic biomarkers in DLBCL and may be included in the initial diagnostic work up of patients with DLBCL.
Matrix for Mucosal Regeneration in Transoral Glossectomy for Squamous Cell Carcinoma: Objective and Subjective Functional Evaluation
Authors: Deganello Alberto , Bosio Paolo , Giannini Lorenzo , Parolini Federico , Berretti Giulia , Sordi Alessandra , Rampinelli Vittorio , Gualtieri Tommaso
Published: January 2023

Background: Numerous options to manage local reconstruction following transoral partial glossectomy are possible. In this work, we present our experience using a matrix for mucosal regeneration, Integra®, after transoral resections of squamous cell carcinoma of the oral tongue. Methods: A retrospective analysis of patients treated for tongue carcinoma and reconstruction with Integra®, from September 2017 to September 2022. Functional outcomes were evaluated by measuring swallowing and speech abilities, tongue motility, and subjective quality of life. Results: The series accounts for 13 consecutive patients, staged from Tis to T3, no positive resection margins were found, average defect size was 17.8 cm2. The average histologically measured depth of invasion was 4.1 mm (range 2–12 mm), and no recurrences were observed during follow-up. All patients maintained excellent swallowing function, the average number of recognized words by an external listener during a phone call was 70.5 out of 75, the lingual motility test was good (a mean score of 4.5 out of 6 movements correctly executed) and subjective questionnaires results were optimal. Less satisfying functional results were recorded in elderly patients receiving a wider surgical resection. Conclusions: This reconstructive technique for allows obtaining optimal healing and functional outcomes in patients with tumors suitable for transoral glossectomy.
Potential Molecular Targeted Therapy for Unresectable Hepatocellular Carcinoma
Authors: Kumar Shashank , Pandey Abhay Kumar
Published: January 2023

Hepatocellular carcinoma (HCC) is one of the most prevalent and lethal cancers, representing a serious worldwide health concern. The recurrence incidence of hepatocellular carcinoma (HCC) following surgery or ablation is as high as 70%. Thus, the clinical applicability of standard surgery and other locoregional therapy to improve the outcomes of advanced HCC is restricted and far from ideal. The registered trials did not identify a treatment that prolonged recurrence-free survival, the primary outcome of the majority of research. Several investigator-initiated trials have demonstrated that various treatments extend patients’ recurrence-free or overall survival after curative therapies. In the past decade, targeted therapy has made significant strides in the treatment of advanced HCC. These targeted medicines produce antitumour effects via specific signals, such as anti-angiogenesis or advancement of the cell cycle. As a typical systemic treatment option, it significantly improves the prognosis of this fatal disease. In addition, the combination of targeted therapy with an immune checkpoint inhibitor is redefining the paradigm of advanced HCC treatment. In this review, we focused on the role of approved targeted medicines and potential therapeutic targets in unresectable HCC.
The Grassland Fragmentation Experiment in the Swiss Jura Mountains: A Synthesis
Authors: Baur Bruno , Rusterholz Hans-Peter , Braschler Brigitte
Published: January 2023

We synthesize findings from a 7-year fragmentation experiment in species-rich, nutrient-poor, dry calcareous grasslands in the north-western Jura mountains, Switzerland. We used a standardized approach with 48 fragments (0.25–20.25 m2) and corresponding control plots in three sites. The 5-m-wide isolation area around the fragments was maintained by frequent mowing. Fragments experienced various ecological changes, e.g., plant biomass increased along fragment edges. We examined fragmentation effects on species richness and composition, abundance, genetic diversity, functional diversity, species traits and species interactions (pollination, herbivory, parasitism, disease) in a wide array of invertebrate (gastropods, spiders, woodlice, various insect groups) and plant taxa. Responses to fragmentation differed between taxonomical groups and species. While species richness and individual density were lower in fragments in some groups, the opposite was true for other groups. Fragmentation effects were most pronounced on species interactions; however, some effects only occurred with a delay. For example, fragmentation influenced foraging patterns of bumblebees, affecting pollination, which in turn resulted in a decreased outcrossing frequency and reduced genetic diversity in a focal plant species. We highlight key findings of the experiment and emphasize their implications for grassland conservation.
Acknowledgment to the Reviewers of Dairy in 2022
Authors:   Dairy Editorial Office
Published: January 2023

High-quality academic publishing is built on rigorous peer review [...]
Feature Transformation for Efficient Blood Glucose Prediction in Type 1 Diabetes Mellitus Patients
Authors: Butt Hatim , Khosa Ikramullah , Iftikhar Muhammad Aksam
Published: January 2023

Diabetes Mellitus, a metabolic disease, causes the body to lose control over blood glucose regulation. With recent advances in self-monitoring systems, a patient can access their personalized glycemic profile and may utilize it for efficient prediction of future blood glucose levels. An efficient diabetes management system demands the accurate estimation of blood glucose levels, which, apart from using an appropriate prediction algorithm, depends on discriminative data representation. In this research work, a transformation of event-based data into discriminative continuous features is proposed. Moreover, a multi-layered long short-term memory (LSTM)-based recurrent neural network is developed for the prediction of blood glucose levels in patients with type 1 diabetes. The proposed method is used to forecast the blood glucose level on a prediction horizon of 30 and 60 min. The results are evaluated for three patients using the Ohio T1DM dataset. The proposed scheme achieves the lowest RMSE score of 14.76 mg/dL and 25.48 mg/dL for prediction horizons of 30 min and 60 min, respectively. The suggested methodology can be utilized in closed-loop systems for precise insulin delivery to type 1 patients for better glycemic control.
Retinal Blood-Vessel Extraction Using Weighted Kernel Fuzzy C-Means Clustering and Dilation-Based Functions
Authors: Wisaeng Kittipol
Published: January 2023

Automated blood-vessel extraction is essential in diagnosing Diabetic Retinopathy (DR) and other eye-related diseases. However, the traditional methods for extracting blood vessels tend to provide low accuracy when dealing with difficult situations, such as extracting both micro and large blood vessels simultaneously with low-intensity images and blood vessels with DR. This paper proposes a complete preprocessing method to enhance original retinal images before transferring the enhanced images to a novel blood-vessel extraction method by a combined three extraction stages. The first stage focuses on the fast extraction of retinal blood vessels using Weighted Kernel Fuzzy C-Means (WKFCM) Clustering to draw the vessel feature from the retinal background. The second stage focuses on the accuracy of full-size images to achieve regional vessel feature recognition of large and micro blood vessels and to minimize false extraction. This stage implements the mathematical dilation operator from a trained model called Dilation-Based Function (DBF). Finally, an optimal parameter threshold is empirically determined in the third stage to remove non-vessel features in the binary image and improve the overall vessel extraction results. According to evaluations of the method via the datasets DRIVE, STARE, and DiaretDB0, the proposed WKFCM-DBF method achieved sensitivities, specificities, and accuracy performances of 98.12%, 98.20%, and 98.16%, 98.42%, 98.80%, and 98.51%, and 98.89%, 98.10%, and 98.09%, respectively.
A Survey on Deep-Learning-Based Diabetic Retinopathy Classification
Authors: Sebastian Anila , Elharrouss Omar , Al-Maadeed Somaya , Almaadeed Noor
Published: January 2023

The number of people who suffer from diabetes in the world has been considerably increasing recently. It affects people of all ages. People who have had diabetes for a long time are affected by a condition called Diabetic Retinopathy (DR), which damages the eyes. Automatic detection using new technologies for early detection can help avoid complications such as the loss of vision. Currently, with the development of Artificial Intelligence (AI) techniques, especially Deep Learning (DL), DL-based methods are widely preferred for developing DR detection systems. For this purpose, this study surveyed the existing literature on diabetic retinopathy diagnoses from fundus images using deep learning and provides a brief description of the current DL techniques that are used by researchers in this field. After that, this study lists some of the commonly used datasets. This is followed by a performance comparison of these reviewed methods with respect to some commonly used metrics in computer vision tasks.
The Role of mpMRI in the Assessment of Prostate Cancer Recurrence Using the PI-RR System: Diagnostic Accuracy and Interobserver Agreement in Readers with Different Expertise
Authors: Bergaglio Chiara , Giasotto Veronica , Marcenaro Michela , Barra Salvina , Turazzi Marianna , Bauckneht Matteo , Casaleggio Alessandro , Sciabà Francesca , Terrone Carlo , Mantica Guglielmo , Borghesi Marco , Signori Alessio , Spina Bruno , Piol Nataniele , Zanardi Elisa , Fornarini Giuseppe , Zawaideh Jeries Paolo
Published: January 2023

Background: treated prostate cancer (PCa) patients develop biochemical recurrence (BCR) in 27–53% of cases; the role of MRI in this setting is still controversial. In 2021 a panel of experts proposed a “Prostate Imaging-Recurrence Reporting” (PI-RR) score, aiming to standardize the reporting. The aim of our study is to evaluate the reproducibility of the PI-RR scoring system among readers with different expertise. Methods: in this monocentric, retrospective observational study, the images of patients who underwent MRI with BCR from January 2017 to January 2022 were analyzed by two radiologists and a radiology resident. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy were obtained. Interobserver agreement was calculated. The percentage of the PI-RR score of 3 was estimated to find out the proportion of uncertain exams reported among the readers. Results: a total of seventy-six patients were included in our study: eight previously treated with RT and sixty-eight who underwent surgery. The accuracy range was 75–80%, the sensitivity 68.4–71.1%, the specificity 81.6–89.5%, PPV 78.8–87.1%, and NPV 72.1–75.6%. The inter-reader agreement using a binary evaluation (PI-RR ≥ 3 as positive mpMRI) demonstrated a correlation coefficient (k) of 0.74 (95% CI: 0.62–0.87). The percentage for the PI-RR score of 3 was 6.6% for reader one, 14.5% for reader two, and 2.6% for reader three. Conclusion: this study confirmed the good accuracy of mpMRI in the detection of local recurrence of PCa and the good reproducibility of PI-RR score among all readers, confirming it to be a promising tool for the standardization of the assessment of patients with BCR.
The Impact of Laser Thermal Effect on Histological Evaluation of Oral Soft Tissue Biopsy: Systematic Review
Authors: Tenore Gianluca , Mohsen Ahmed , Nuvoli Alessandro , Palaia Gaspare , Rocchetti Federica , Di Gioia Cira Rosaria Tiziana , Cicconetti Andrea , Romeo Umberto , Del Vecchio Alessandro
Published: January 2023

The aim of the study is to review the literature to observe studies that evaluate the extent of the thermal effect of different laser wavelengths on the histological evaluation of oral soft tissue biopsies. An electronic search for published studies was performed on the PubMed and Scopus databases between July 2020 and November 2022. After the selection process, all the included studies were subjected to quality assessment and data extraction processes. A total of 28 studies met the eligibility criteria. The most studied laser was the carbon dioxide (CO2) laser, followed by the diode laser 940 nm–980 nm. Six studies were focused on each of the Erbium-doped Yttrium Aluminium Garnet (Er:YAG), Neodymium-doped Yttrium Aluminum Garnet (Nd:YAG) lasers, and diode lasers of 808 nm and 445 nm. Three studies were for the Potassium Titanyl Phosphate (KTP) laser, and four studies were for the Erbium, Chromium-doped Yttrium, Scandium, Gallium, and Garnet (Er,Cr:YSGG) laser. The quality and bias assessment revealed that almost all the animal studies were at a low risk of bias (RoB) in the considered domains of the used assessment tool except the allocation concealment domain in the selection bias and the blinding domain in the performance bias, where these domains were awarded an unclear or high score in almost all the included animal studies. For clinical studies, the range of the total RoB score in the comparative studies was 14 to 23, while in the non-comparative studies, it was 11 to 15. Almost all the studies concluded that the thermal effect of different laser wavelengths did not hinder the histological diagnosis. This literature review showed some observations. The thermal effect occurred with different wavelengths and parameters and what should be done is to minimize it by better adjusting the laser parameters. The extension of margins during the collection of laser oral biopsies and the use of laser only in non-suspicious lesions are recommended because of the difficulty of the histopathologist to assess the extension and grade of dysplasia at the surgical margins. The comparison of the thermal effect between different studies was impossible due to the presence of methodological heterogeneity.
Superradiant Quantum Phase Transition for an Exactly Solvable Two-Qubit Spin-Boson Model
Authors: Grimaudo Roberto , Valenti Davide , Sergi Alessandro , Messina Antonino
Published: January 2023

A spin-boson-like model with two interacting qubits is analysed. The model turns out to be exactly solvable since it is characterized by the exchange symmetry between the two spins. The explicit expressions of eigenstates and eigenenergies make it possible to analytically unveil the occurrence of first-order quantum phase transitions. The latter are physically relevant since they are characterized by abrupt changes in the two-spin subsystem concurrence, in the net spin magnetization and in the mean photon number.
Maximum Entropy Exploration in Contextual Bandits with Neural Networks and Energy Based Models
Authors: Elwood Adam , Leonardi Marco , Mohamed Ashraf , Rozza Alessandro
Published: January 2023

Contextual bandits can solve a huge range of real-world problems. However, current popular algorithms to solve them either rely on linear models or unreliable uncertainty estimation in non-linear models, which are required to deal with the exploration–exploitation trade-off. Inspired by theories of human cognition, we introduce novel techniques that use maximum entropy exploration, relying on neural networks to find optimal policies in settings with both continuous and discrete action spaces. We present two classes of models, one with neural networks as reward estimators, and the other with energy based models, which model the probability of obtaining an optimal reward given an action. We evaluate the performance of these models in static and dynamic contextual bandit simulation environments. We show that both techniques outperform standard baseline algorithms, such as NN HMC, NN Discrete, Upper Confidence Bound, and Thompson Sampling, where energy based models have the best overall performance. This provides practitioners with new techniques that perform well in static and dynamic settings, and are particularly well suited to non-linear scenarios with continuous action spaces.
Scarring in Rough Rectangular Billiards
Authors: Izrailev Felix M. , Luna-Acosta German A. , Mendez-Bermudez J. A.
Published: January 2023

We study the mechanism of scarring of eigenstates in rectangular billiards with slightly corrugated surfaces and show that it is very different from that known in Sinai and Bunimovich billiards. We demonstrate that there are two sets of scar states. One set is related to the bouncing ball trajectories in the configuration space of the corresponding classical billiard. A second set of scar-like states emerges in the momentum space, which originated from the plane-wave states of the unperturbed flat billiard. In the case of billiards with one rough surface, the numerical data demonstrate the repulsion of eigenstates from this surface. When two horizontal rough surfaces are considered, the repulsion effect is either enhanced or canceled depending on whether the rough profiles are symmetric or antisymmetric. The effect of repulsion is quite strong and influences the structure of all eigenstates, indicating that the symmetric properties of the rough profiles are important for the problem of scattering of electromagnetic (or electron) waves through quasi-one-dimensional waveguides. Our approach is based on the reduction of the model of one particle in the billiard with corrugated surfaces to a model of two artificial particles in the billiard with flat surfaces, however, with an effective interaction between these particles. As a result, the analysis is conducted in terms of a two-particle basis, and the roughness of the billiard boundaries is absorbed by a quite complicated potential.
Implementation of the Simple Hyperchaotic Memristor Circuit with Attractor Evolution and Large-Scale Parameter Permission
Authors: Yang Gang , Zhang Xiaohong , Moshayedi Ata Jahangir
Published: January 2023

A novel, simple, four-dimensional hyperchaotic memristor circuit consisting of two capacitors, an inductor and a magnetically controlled memristor is designed. Three parameters (a, b, c) are especially set as the research objects of the model through numerical simulation. It is found that the circuit not only exhibits a rich attractor evolution phenomenon, but also has large-scale parameter permission. At the same time, the spectral entropy complexity of the circuit is analyzed, and it is confirmed that the circuit contains a significant amount of dynamical behavior. By setting the internal parameters of the circuit to remain constant, a number of coexisting attractors are found under symmetric initial conditions. Then, the results of the attractor basin further confirm the coexisting attractor behavior and multiple stability. Finally, the simple memristor chaotic circuit is designed by the time-domain method with FPGA technology and the experimental results have the same phase trajectory as the numerical calculation results. Hyperchaos and broad parameter selection mean that the simple memristor model has more complex dynamic behavior, which can be widely used in the future, in areas such as secure communication, intelligent control and memory storage.
Measurement-Based Quantum Thermal Machines with Feedback Control
Authors: Bhandari Bibek , Czupryniak Robert , Erdman Paolo Andrea , Jordan Andrew N.
Published: January 2023

We investigated coupled-qubit-based thermal machines powered by quantum measurements and feedback. We considered two different versions of the machine: (1) a quantum Maxwell’s demon, where the coupled-qubit system is connected to a detachable single shared bath, and (2) a measurement-assisted refrigerator, where the coupled-qubit system is in contact with a hot and cold bath. In the quantum Maxwell’s demon case, we discuss both discrete and continuous measurements. We found that the power output from a single qubit-based device can be improved by coupling it to the second qubit. We further found that the simultaneous measurement of both qubits can produce higher net heat extraction compared to two setups operated in parallel where only single-qubit measurements are performed. In the refrigerator case, we used continuous measurement and unitary operations to power the coupled-qubit-based refrigerator. We found that the cooling power of a refrigerator operated with swap operations can be enhanced by performing suitable measurements.
Media Education through Digital Games: A Review on Design and Factors Influencing Learning Performance
Authors: Ishak Shahrul Affendi , Hasran Umi Azmah , Din Rosseni
Published: January 2023

In the past two decades, a considerable amount of research has focused on digital games as part of media education. Digital game-based learning (DGBL) is identified as a potential pedagogical approach to enhance learning in the digital era. In light of the increased number of academic articles on educational games, this study considers the development and the factors that influence learning performance among children by providing a thematic review of the literature available on DGBL. Findings show that despite following developmental standard procedures, a distinct perspective between the game designer and the educator is essential. Three major approaches have been identified, namely the learner-centered, the game-centered and the model approaches. Learning processes that use digital games show empirically positive results as well as the great influence in terms of intrinsic motivation, game features and overall learning experience.