bluuebunny/crossref_metadata_2025 huggingface dataset:
Roles and Challenges for Village Doctors in COVID-19 Pandemic Prevention and Control in Rural Beijing, China: A Qualitative Study
Authors: Li Jin , Zhao Ning , Zhang Haiyan , Yang Hui , Yang Jia
Published: June 2022

Objectives

Rural areas in China are more vulnerable to COVID-19 pandemic than urban areas, due to their far fewer health care resources. Village doctors, as rural grassroots health workers in China, have been actively engaged in the pandemic prevention and control. This study aims to describe the roles of village doctors in rural China, and the challenges they have faced during the prevention and control of the COVID-19 pandemic.

Setting

This study was conducted in three towns in Huairou District, Beijing, China.

Design

We carried out semi-structured interviews with 75 key informants. All the interviews were audio-recorded and transcribed verbatim. We employed thematic analysis to define themes and sub-themes from the qualitative data.

Results

We reported four themes. First, the village doctor guided the village committee to carry out decontamination, monitored home-isolated residents, and disseminated knowledge on prevention of the COVID-19 pandemic during the rural pandemic prevention and control. Second, they took pandemic prevention measures in village clinics, distributed pandemic prevention materials, and undertook pre-screening triage. Third, village doctors provided basic medical care, including treatment of common diseases as well as the purchase and delivery of medicines to villagers. Fourth, village doctors faced difficulties and challenges, such as inadequate medical skills, aging staff structure, and lack of pandemic prevention materials.

Conclusions

Despite many difficulties and challenges, village doctors have actively participated in rural pandemic prevention and control, and made outstanding contributions to curbing spread of COVID-19 pandemic in rural areas. Village doctors provide basic health care while participating in various non-medical tasks.
COVID-19 in China: A Rapid Review of the Impacts on the Mental Health of Undergraduate Students
Authors: Shi Hairong , Zhu Haixue , Ni Yan
Published: June 2022

Public health crises pose challenges for governments and health systems, and the coronavirus disease 2019 (COVID-19) has presented major challenges to humans worldwide. In the context of COVID-19 in China, we explore the impacts of the pandemic on the mental health of undergraduate students. We examine pandemic prevention and control measures in Chinese universities through a rapid review and use our findings to explain the difficulties that undergraduate students face. Moreover, our analysis examines the impacts on five aspects of mental health: emotional aspects, personality, interpersonal relationships, learning behavior and employment options. Additionally, we provide implications in four areas based on the application of the study: strengthening psychological intervention, promoting government information disclosure, improving family communication and adjusting self-awareness.
Planetary nebulae and how to find them: A concise review
Authors: Parker Quentin A.
Published: June 2022

This review provides useful background and information on how we find, vet and compile Planetary Nebulae (PNe) candidates and verify them. It presents a summary of the known Galactic PNe population and their curation in the Hong Kong/AAO/Strasbourg/Hα PNe catalogue, “HASH”. It is a simple introduction for anyone interested in working with PNe, including postgraduate students entering the field and for more general interest too.
Surgical Correction of Kyphosis in Patients With Camptocormia Associated With Parkinson’s Disease: A Case Report and Review of the Literature
Authors: Mei Guo-long , Wei Hui-ting , Ma Yue-rong , Wan Dun
Published: June 2022

Background

Camptocormia is a postural deformity that is characterized by a markedly flexed lumbar spine, with symptoms that worsen with walking and standing. Here, we report a case of camptocormia associated with Parkinson’s disease.

Case description

A 70-year-old man with a 7-year history of Parkinson’s disease presented with a fall injury that caused lower back pain for 3 months and was aggravated for 2 months. He had been diagnosed with a compression fracture after the fall and had undergone percutaneous kyphoplasty at a local hospital. MRI showed non-union of the L1 vertebra and compression fracture of L2. The patient underwent posterior osteotomy, canal decompression, and internal fixation of the T10-L3 intervertebral plate with bone graft fusion. Postoperative examination showed that the lumbar lordosis was corrected and sensation was restored in both lower extremities. However, after 1 month, the fixation was loosened and a correction surgery was performed at our hospital. At the most recent follow-up at 1.5 years, the patient was found to be in good general health and did not complain of lower back discomfort. He was also actively exercising according to the rehabilitation regimen and had resumed social life.

Conclusion

This is a rare case of camptocormia in a Parkinson’s patient that highlights the need for careful evaluation of whether internal spinal fixation surgery is beneficial in such patients.
MRI-Based Optimization Design of the Pre-Spinal Route of Contralateral C7 Nerve Transfer for Spastic Arm Paralysis
Authors: Zhao Hua-Li , Gao Yun , Yu Ai-Ping , Wei Yi-Min , Shen Yun-Dong , Jiang Su , Qiu Yan-Qun , Yu Jing , Liang Zong-Hui
Published: June 2022

Purpose

The prespinal route of contralateral cervical 7 nerve transfer developed by Prof. Wendong Xu helps realize the direct anastomosis of the bilateral cervical 7 nerves. However, 20% of operations still require a nerve graft, which leads to an unfavorable prognosis. This study aims to explore the optimized prespinal route with MRI to further improve the prognosis.

Methods

The current study enrolled 30 patients who suffered from central spastic paralysis of an upper limb and who underwent contralateral cervical 7 nerve transfer via Prof. Xu’s prespinal route through the anterior edge of the contralateral longus colli. MRI images were used to analyze the route length, vertebral artery exposure, and contralateral cervical 7 nerve included angle. Three prespinal routes were virtually designed and analyzed. The selected optimal route was applied to another 50 patients with central spastic paralysis of an upper limb for contralateral cervical 7 nerve transfer.

Results

By the interventions on the 30 patients, the middle and posterior routes were shorter than the anterior route in length, but with no statistical difference between the two routes. Of 30 contralateral vertebral arteries, 26 were located at the posterior medial edge of the longus colli. The average included angles of the anterior, middle, and posterior routes were 108.02 ± 7.89°, 95.51 ± 6.52°, and 72.48 ± 4.65°, respectively. According to these data, the middle route was optimally applied to 50 patients, in whom the rate of nerve transplantation was only 4%, and no serious complications such as vertebral artery or brachial plexus injury occurred.

Conclusion

The low rate of nerve transplantation in 50 patients and the absence of any serious complications in these cases suggests that the middle route is the optimal one.
Robotic Adrenalectomy: An Initial Experience in a Turkish Regional Hospital
Authors: Erdemir Ayhan , Rasa Kemal
Published: June 2022

Background

Due to the technical advantages and the convenience it provides to surgeons, “robotic adrenalectomy” is a widely used procedure for adrenal surgeries. In this study, we aim to evaluate our robotic adrenalectomy experience and delineate the factors that have a substantial impact on surgical outcomes.

Methods

Successive 0 transperitoneal robotic operations using the daVinci SI® platform were grouped according to the surgery side, malignant or benign pathologies, for adenoma or non-adenoma lesions, tumor size of less than 4 cm or above, body mass index below or above 30 kg/m2, and with or without laparotomy history. Groups were compared in terms of duration of the operations, amount of bleeding, and the duration of hospitalization.

Results

Morbidity developed in 5 patients (16.6%), and no mortality was observed. We had only one conversion to perform open surgery (3.3%). Operations performed for adenoma significantly last longer when compared with the non-adenoma group (p < 0.05). In the malignant group, the amount of bleeding during surgery was found to be significantly higher (p < 0.05). The blood loss during the surgery was also found to be higher in the adenoma group than in the non-adenoma match (p < 0.05). Phenomenally, operative blood loss was found to be lesser in the bigger tumor size group (>4 cm) than in the smaller size group (p < 0.05).

Conclusion

Our results corroborate that robotic adrenalectomy may be more challenging for malignant pathologies and adenomas, but we can claim that it is an effective and safe option for all adrenal gland pathologies.
The Learning Curve of Da Vinci Robot-Assisted Hemicolectomy for Colon Cancer: A Retrospective Study of 76 Cases at a Single Center
Authors: Huang Pu , Li Sen , Li Peng , Jia Baoqing
Published: June 2022

Background and Aims

Robotic-assisted right hemicolectomy (RARH) has many benefits in treating colon cancer, but it is a new technology that needs to be evaluated. This study aims to assess the learning curve (LC) of RARH procedures with the complete mesoscopic exception and D3 lymph node dissection for colon carcinoma.

Methods

A retrospective analysis was performed on a consecutive series of 76 patients who underwent RARH from July 2014 to March 2018. The operation time was evaluated using the cumulative sum (CUSUM) method to analyze the LC. The patients were categorized into two groups based on the LC: Phase I and Phase II. Statistical methods were used to compare clinicopathological data on intraoperative and perioperative outcomes at different stages of the study.

Results

The peak point of the LC was observed in the 27th case. Using the CUSUM method, we divide the LC into two stages. Stage 1 (initial learning stage): Cases 1–27 and Stage 2 (proficiency phase): Cases 28–76. There were no obvious distinctions between the two patients’ essential characteristics (age, sex, body mass index, clinical stage, and ASA score). The mean operation time of each group is 187.37 ± 45.56 min and 161.1 ± 37.74 min (P = 0.009), respectively. The intraoperative blood loss of each group is 170.4 ± 217.2 ml and 95.7 ± 72.8 ml (P = 0.031), respectively.

Conclusion

Based on the LC with CUSUM analysis, the data suggest that the learning phase of RARH was achieved after 27 cases. The operation time and the intraoperative blood loss decrease with more cases performed.
Spontaneous Rupture and Hemorrhage of WON: A Case Report
Authors: Yan Jican , Yu Wenhao , Yan Jingxin , Guo Xinjian , Hou Lizhao , Ren Li , Fan Haining
Published: June 2022

Background

Pancreatic pseudocysts are characterized by the leakage of pancreatic juice caused by various reasons, which leads to pancreatic juice accumulates around the pancreas, and stimulates the greater omentum and other tissues to form an area of fibrotic loculated effusion with an integrated capsule. Approximately, one-third of patients experienced recurrent pancreatic juice leakage, compression symptoms, infection, and bleeding, which requires surgical intervention, but spontaneous rupture cases are extremely rare.

Case presentation

We here present the case of 40-year-old male who presented with abdominal pain and vomiting for two days and 10 h, respectively. He had a history of chronic pancreatitis and pseudocysts. The symptoms of abdominal pain worsened in the second day. Laboratory tests showed a progressive decrease in hemoglobin. Then, emergency pancreatoduodenectomy was performed. Intraoperative exploration found a small blood clot in the abdominal cavity and a hematoma that had formed in the intestinal cavity and retroperitoneum.

Conclusion

This case showed that pseudocysts of the pancreas can rupture under certain circumstances, leading to intraperitoneal bleeding and hematoma formation, which can endanger the life of the patient. And surgical treatment can be the first choice for hemorrhagic pseudocysts.
Biomechanical and Microstructural Properties of Subchondral Bone From Three Metacarpophalangeal Joint Sites in Thoroughbred Racehorses
Authors: Pearce Duncan J. , Hitchens Peta L. , Malekipour Fatemeh , Ayodele Babatunde , Lee Peter Vee Sin , Whitton R. Chris
Published: June 2022

Fatigue-induced subchondral bone (SCB) injury is common in racehorses. Understanding how subchondral microstructure and microdamage influence mechanical properties is important for developing injury prevention strategies. Mechanical properties of the disto-palmar third metacarpal condyle (MCIII) correlate poorly with microstructure, and it is unknown whether the properties of other sites within the metacarpophalangeal (fetlock) joint are similarly complex. We aimed to investigate the mechanical and structural properties of equine SCB from specimens with minimal evidence of macroscopic disease. Three sites within the metacarpophalangeal joint were examined: the disto-palmar MCIII, disto-dorsal MCIII, and proximal sesamoid bone. Two regions of interest within the SCB were compared, a 2 mm superficial and an underlying 2 mm deep layer. Cartilage-bone specimens underwent micro-computed tomography, then cyclic compression for 100 cycles at 2 Hz. Disto-dorsal MCIII specimens were loaded to 30 MPa (n = 10), while disto-palmar MCIII (n = 10) and proximal sesamoid (n = 10) specimens were loaded to 40 MPa. Digital image correlation determined local strains. Specimens were stained with lead-uranyl acetate for volumetric microdamage quantification. The dorsal MCIII SCB had lower bone volume fraction (BVTV), bone mineral density (BMD), and stiffness compared to the palmar MCIII and sesamoid bone (p < 0.05). Superficial SCB had higher BVTV and lower BMD than deeper SCB (p < 0.05), except at the palmar MCIII site where there was no difference in BVTV between depths (p = 0.419). At all sites, the deep bone was stiffer (p < 0.001), although the superficial to deep gradient was smaller in the dorsal MCIII. Hysteresis (energy loss) was greater superficially in palmar MCIII and sesamoid (p < 0.001), but not dorsal MCIII specimens (p = 0.118). The stiffness increased with cyclic loading in total cartilage-bone specimens (p < 0.001), but not in superficial and deep layers of the bone, whereas hysteresis decreased with the cycle for all sites and layers (p < 0.001). Superficial equine SCB is uniformly less stiff than deeper bone despite non-uniform differences in bone density and damage levels. The more compliant superficial layer has an important role in energy dissipation, but whether this is a specific adaptation or a result of microdamage accumulation is not clear.
Study on Numerical Algorithm of the N-S Equation for Multi-Body Flows around Irregular Disintegrations in Near Space
Authors: Han Zheng , Li Zhihui , Bai Zhiyong , Li Xuguo , Zhang Jiazhong
Published: June 2022

There has been a concern that the accurate numerical simulation of multi-body flow, which is caused by the multiple disintegrations of expired spacecraft re-entering into the near space, has a critical bottleneck impact on the falling area of the disintegrated debris. To solve this problem, an O-type grid topology method has been designed for the multi-body flow field of irregular debris formed by multiple disintegrations in near space, and a finite-volume implicit numerical scheme has been constructed for the Navier-Stokes equations to solve the aerodynamic interference characteristics of irregular multi-body flow, and further the N-S equation numerical algorithm has been established for the irregular multi-body flows in near space. The reliability of the method has been verified by the comparison of the present computation and the experiment of the low-density wind tunnel for the two-body flow of sphere, cylinder and square scripts. The objects of this study are from the multiple disintegrations of the Tiangong-1 spacecraft during uncontrolled re-entry into the atmosphere, including propelling cylinders and low-temperature lock cabinets. A series of simulations of multi-body flow mechanisms around different combinations have been carried out with varied shapes and spacing. As a result, it is found that when the distance of irregular debris (e.g., two propelling cylinders) in the near space is in the range of Δy < 3D or Δx < D, there is an obvious multi-body interference between debris, and the flow characteristics are obviously changed. When the distance between the debris in near space reaches a certain level, the influence of mutual interference can be ignored. For example, when the y-direction distance between multiple bodies is greater than 3D, the flow interference tends to be small and can be ignored, and we can regard them as two separate pieces to be carried out by the numerical prediction of flight track and falling area in engineering application. The results provide a practical design criterion for the integrated simulation platform which is used to simulate the multi-physics complex aerodynamics of space vehicles from the free-molecule flow of the outer space to the near-ground continuum flow.
Design and Parameter Optimization of a Finger Clip Plate Garlic Seed-Metering Device Based on EDEM
Authors: Wang Haoyi , Sun Xinping , Li Hua , Fu Jieyi , Zeng Xiaoping , Xu Youzhi , Wang Yongjian , Liu Huaqing , Lü Zhimin
Published: June 2022

In present-day mechanized garlic seeding, high missing rates and low qualified percentages of single seeds are common problems; thus, a finger clip plate garlic seed-metering device was designed in this study. First, the structure and working principle of the seed-metering device were studied. Subsequently, the critical component parameters of the seed-metering device were determined using theoretical calculations; then, EDEM software was used in single-factor simulation experiments to analyze the effects of opening the diameter of the seed-collecting spoon, the operating speed of the seeding tray, and the population number on the seed-filling performance. Finally, a Box–Behnken center combination experiment was conducted with the population, opening the diameter of the seed-collecting spoon, and rotating the speed of the seeding tray as experiment factors, with the single-seed filling rate, qualified percent, and missing rate as evaluation indicators. A three-factor and three-level orthogonal test was conducted to establish the mathematic regression model of the experiment factors and evaluation indicators, as well as to realize the parameter optimization. After rounding, the laboratory validation test was carried out with 240~280 seeds, a 26 mm seed scoop-opening diameter, and a 28 r/min operating speed. The average qualified rate, missing rate, and reply rate of single seeds were 91.86%, 2.71%, and 5.43%, respectively, which is basically consistent with the forecast results of regression model.
Identification of SSR Markers Associated with Yield-Related Traits and Heterosis Effect in Winter Oilseed Rape (Brassica Napus L.)
Authors: Wolko Joanna , Łopatyńska Agnieszka , Wolko Łukasz , Bocianowski Jan , Mikołajczyk Katarzyna , Liersch Alina
Published: June 2022

The identification of markers responsible for regulating important agronomic traits in rapeseed supports breeding and increases the seed yield. Microsatellite (SSR) markers are mainly used as ‘neutral’ genetic markers but are also linked with many biological functions. The objective of this study was identification of microsatellite markers associated with important agronomic traits affecting the seed yield of winter oilseed rape and with the heterosis effect for these traits. The plant material consists of four parental lines, 60 doubled haploid (DH) lines, 60 single cross hybrids, and 60 three-way cross hybrids. The association between molecular markers and observed traits was estimated using regression analysis. Among 89 SSR markers, 43 were polymorphic, and 15 were selected for mapping because they demonstrated stability in both years of observation. These markers were physically mapped in the rapeseed reference genomes and their immediate vicinity was searched to identify candidate genes associated with the studied traits. Six markers (BrGMS3837, BnEMS1119, BrGMS2901, BnGMS0509, BrGMS3688, BrGMS4057), which showed a positive estimation effect in our association analysis, and thus increased the value of a given trait or heterosis effect, turned out to be linked with genes that could be responsible for the development and growth of plants.
Better Drought Index between SPEI and SMDI and the Key Parameters in Denoting Drought Impacts on Spring Wheat Yields in Qinghai, China
Authors: Hou Miaolei , Yao Ning , Li Yi , Liu Fenggui , Biswas Asim , Pulatov Alim , Hassan Ishtiaq
Published: June 2022

Drought has great negative impacts on crop growth and production. In order to select appropriate drought indices to quantify drought influences on crops to minimize the risk of drought-related crops as much as possible, climate and spring wheat yield-related data from eight sites in the Qinghai Province of China were collected for selecting better drought index between standardized precipitation evapotranspiration index (SPEI, denoting meteorological drought) and soil moisture deficit index (SMDI, denoting agricultural drought) as well as the key parameters (timescale and month) in denoting drought impacts on spring wheat yields. The spring wheat yields during 1961–2018 were simulated by the DSSAT–CERES–Wheat model. Pearson correlations were used to investigate the relationship between SPEI and SMDI and between spring wheat yields and drought indices at different timescales. The results showed that: (1) SMDI reflected more consistent dry/wet conditions than SPEI when the timescales changed and (2) There were one- and two-month lags in SMDI compared to SPEI (with the higher correlation coefficients values of 0.35–0.68) during May to August and (3) May (the jointing period of spring wheat) and the two-month timescale of SMDI0–10 (with the higher correlation coefficients values of 0.21–0.37) were key parameters denoting drought influences on spring wheat yield and (4) The correlations between the linear slopes of spring wheat yield reduction rate and linear slopes of SMDI0–10 in May at the studied eight sites were considerable between 1961–2018 (r = 0.85). This study provides helpful references for mitigating the drought risk of spring wheat.
Guidelines for Telemetry Studies on Snow Leopards
Authors: Johansson Örjan , Kachel Shannon , Weckworth Byron
Published: June 2022

Animal-borne tracking devices have generated a wealth of new knowledge, allowing us to better understand, manage and conserve species. Fitting such tracking devices requires that animals are captured and often chemically immobilized. Such procedures cause stress and involve the risk of injuries and loss of life even in healthy individuals. For telemetry studies to be justifiable, it is vital that capture operations are planned and executed in an efficient and ethical way. Project objectives must be clearly articulated to address well-defined knowledge gaps, and studies designed to maximize the probability of achieving those goals. We provide guidelines for how to plan, design, and implement telemetry studies with a special emphasis on snow leopards that are typically captured using foot snares. We also describe the necessary steps to ensure that captures are conducted safely, and with minimal stress to animals.
Effects of Replacing Fishmeal and Soybean Protein Concentrate with Degossypolized Cottonseed Protein in Diets on Growth Performance, Nutrient Digestibility, Intestinal Morphology, Cecum Microbiome and Fermentation of Weaned Piglets
Authors: Wang Li , Gao Wenjun , Shi Huangwei , Hu Qile , Lai Changhua
Published: June 2022

The inclusion of high-quality proteins is commonly used in swine production, especially in weaned pigs. Our research investigated the effects of replacing fishmeal (FM) and soybean protein concentrate (SPC) with degossypolized cottonseed protein (DCP) on the growth performance, nutrient digestibility, intestinal morphology, cecum microbiota and fermentation in weaned pigs. A total of 90 pigs were used in a 4-week trial. Pigs were randomly assigned to three dietary treatments (initial BW 8.06 ± 0.26 kg; six pigs per pen; five pens per treatment), including a basal diet group (CON) with a 6% SPC and 6% FM; two experimental diets group (SPCr and FMr) were formulated by replacing SPC or FM with 6% DCP, respectively. There were no differences in growth performance and diarrhea rate among three treatments except for the ADFI during day 14 to day 28. Using the DCP to replace FM would weaken the jejunum morphology and decrease the nutrient digestibility of pigs during day 0 to day 14. However, replacing FM with DCP can improve the community structure of cecum microbiota, and may relieve these negative effects. In conclusion, DCP can be used as a cost-effective alternative protein supplement.
In Vitro Evaluation of Antimicrobial Effect of Phytobiotics Mixture on Salmonella spp. Isolated from Chicken Broiler
Authors: Iwiński Hubert , Wódz Karolina , Chodkowska Karolina , Nowak Tomasz , Różański Henryk
Published: June 2022

Background: The identification of natural antibacterial agents from various sources that can act effectively against disease-causing foodborne bacteria is one of the major concerns throughout the world. In the present study, a unique phytobiotics mixture containing thymol, menthol, linalool, trans-anethole, methyl salicylate, 1,8-cineole, and p-cymene was evaluated for antibacterial activity against selected strains of Salmonella spp. Results: The phytobiotics mixture was effective against Salmonella enterica subsp. enterica serovars Enteritidis, Typhimurium, and Kentucky. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of this unique mixture for these three pathogens were 1:256. Among these three strains, one S. Kentucky presented the most extensive resistance profiles to 18 antibiotics belonging to 5 classes of antibiotics. One of S. Typhimurium presents extensive resistance profiles to 14 antibiotics belonging to 5 classes of antibiotics. Conclusions: The results suggest that the phytobiotics mixture used in the experiment can be used as a strong natural antibacterial agent against Gram-negative foodborne pathogens such as S. Typhimurium, S. Kentucky, and S. Enteritidis. This is a preliminary analysis of the effectiveness of a phytobiotic product in an in vitro model, which may be the starting point for further studies, including in vivo animal models.
Healthcare Professionals’ Knowledge and Beliefs on Antibiotic Prophylaxis in Cesarean Section: A Mixed-Methods Study in Benin
Authors: Dohou Angèle Modupè , Buda Valentina Oana , Anagonou Severin , Van Bambeke Françoise , Van Hees Thierry , Dossou Francis Moïse , Dalleur Olivia
Published: June 2022

A low adherence to recommendations on antibiotic prophylaxis has been reported worldwide. Since 2009, cesarean sections have been performed under user fee exemption in Benin with a free kit containing the required supplies and antibiotics for prophylaxis. Despite the kit, the level of antibiotic prophylaxis achievement remains low. We conducted a convergent parallel design study in 2017 using a self-administered questionnaire and interviews to assess the knowledge and explore the beliefs of healthcare professionals regarding antibiotic prophylaxis in three hospitals. Of the 35 participants, 33 filled out the questionnaire. Based on the five conventional criteria of antibiotic prophylaxis, the mean level of knowledge was 3.3 out of 5, and only 15.2% scored 5 out of 5. From the verbatim of 19 interviewees, determinants such as suboptimal patient status health, low confidence in antibiotics, some disagreement with the policy, inappropriate infrastructures and limited financial resources in hospitals, poor management of the policy in the central level, and patient refusal to buy antibiotics can explain poor practices. Because of the dysfunction at these levels, the patient becomes the major determinant of adequate antibiotic prophylaxis. Policymakers have to consider these determinants for improving antibiotic prophylaxis in a way that ensures patient safety and reduces the incidence of antimicrobial resistance.
Three-Dimensional Dynamic Variations of Ground/Air Surface Temperatures and Their Correlation with Large-Scale Circulation Indexes in Southwest China (1980–2019)
Authors: Jin Hanyu , Cheng Qingping , Wang Ping
Published: June 2022

Air/soil temperatures play important roles in land–atmosphere interactions. The three-dimensional (temporal, spatial, and vertical) variations of maximum, mean, and minimum ground soil temperature at 0 cm (GSTx, GSTm, and GSTn, respectively), surface air temperature at 2 m (SATx, SATm, and SATn, respectively), and soil–air temperature difference (SATDx, SATDm, and SATDn, respectively) and their potential linkages with large-scale indexes in Southwest China during 1980–2019 were analyzed. Variations of GST and SAT at the majority of stations (pixels) exhibited significant (p ≤ 0.05) warming, albeit at different rates; consequently, SATD exhibited different variation. Moreover, the period of GST, SAT, and SATD was similar in intra-annual and interannual oscillation but was different in interdecadal oscillation. The variation rate of GST, SAT, and SATD exhibited significant (p ≤ 0.05) correlation with elevation, but with different variation gradient. Notably, asymmetric variation of SATDx (downward trend) and of SATDn (upward trend) with elevation was found at elevations >3 km. Wavelet coherence showed that the Atlantic Multidecadal Oscillation is the dominant factor affecting GST and SAT, whereas the Pacific Decadal Oscillation and the North Atlantic Oscillation make the greatest contributions to SATD. It was found that GST, SAT, and SATD exhibit different variations under the effects of global warming, the driving mechanism of which requires further study.
Thermal Stability and the Effect of Water on Hydrogen Fluoride Generation in Lithium-Ion Battery Electrolytes Containing LiPF6
Authors: Han Ji Yun , Jung Seungho
Published: June 2022

Lithium-ion batteries (LIBs) have been used as electrochemical energy storage devices in various fields, ranging from mobile phones to electric vehicles. LIBs are composed of a positive electrode, a negative electrode, an electrolyte, and a binder. Among them, electrolytes consist of organic solvents and lithium ion conducting salts. The electrolytes used in LIBs are mostly linear and cyclic alkyl carbonates. These electrolytes are usually based on their combinations to allow the use of Li as the anodic active component, resulting in the high power and energy density of batteries. However, these organic electrolytes have high volatility and flammability that pose a serious safety issue when exposed to extreme conditions such as elevated temperatures. At that time, these electrolytes can react with active electrode materials and release a considerable amount of heat and gas. In this study, a simultaneous thermal analysis-mass spectrometry analysis was performed on six different organic solvents to examine the effect of water on hydrogen fluoride (HF) generation temperature in the electrolyte of a LIB. The electrolytes used in the experiment were anhydrous diethyl carbonate, 1,2-dimethoxyethane, ethylene carbonate, 1,3-dioxolane, tetrahydrofurfuryl alcohol, and 2-methyl-tetrahydrofuran, each containing LiPF6. The HF formation temperature was observed and compared with that when water entered the electrolyte exposed to high-temperature conditions such as fire.
Elastodontic Devices in Orthodontics: An In-Vitro Study on Mechanical Deformation under Loading
Authors: Quinzi Vincenzo , Gallusi Gianni , Carli Elisabetta , Pepe Francesca , Rastelli Elena , Tecco Simona
Published: June 2022

The purpose of the present study was to evaluate the mechanical resistance of elastodontic devices (ED): their maximum compression loads and plastic deformation under loading (percentage). An Instron universal machine (Model 3365, Instron, Industrial Product Group, Grove City, PA, USA) was employed with a 100 N load cell and with Bluehill software for loading analyses. Each device was submitted to a five-cycles test. The following ED were evaluated: A.M.C.O.P. (Micerium, Genova, Italy) in red color, in orange color, and in blue color; HealthyStart (Ortho-Tain, Winnetka, IL, USA), and T4K™ phase 1 (Myofunctional Research Co., Helensvale, Australia). During the five-cycles test, the Ortho-Tain device delivered the greatest compression load (7.56 N), with the lowest percentage of deformation (0.95%). For all devices, a slight plastic deformation of the material was registered, ranging from 0.95% to 1.75%. For the T4K device it was not possible to complete the five-cycles test. For all the analyzed ED, a slight plastic deformation under loading was registered, that in all cases can be considered clinically acceptable. Further studies are needed to test the appliances after clinical usage.
EMD-Based Method for Supervised Classification of Parkinson’s Disease Patients Using Balance Control Data
Authors: Safi Khaled , Aly Wael Hosny Fouad , AlAkkoumi Mouhammad , Kanj Hassan , Ghedira Mouna , Hutin Emilie
Published: June 2022

There has recently been increasing interest in postural stability aimed at gaining a better understanding of the human postural system. This system controls human balance in quiet standing and during locomotion. Parkinson’s disease (PD) is the most common degenerative movement disorder that affects human stability and causes falls and injuries. This paper proposes a novel methodology to differentiate between healthy individuals and those with PD through the empirical mode decomposition (EMD) method. EMD enables the breaking down of a complex signal into several elementary signals called intrinsic mode functions (IMFs). Three temporal parameters and three spectral parameters are extracted from each stabilometric signal as well as from its IMFs. Next, the best five features are selected using the feature selection method. The classification task is carried out using four known machine-learning methods, KNN, decision tree, Random Forest and SVM classifiers, over 10-fold cross validation. The used dataset consists of 28 healthy subjects (14 young adults and 14 old adults) and 32 PD patients (12 young adults and 20 old adults). The SVM method has a performance of 92% and the Dempster–Sahfer formalism method has an accuracy of 96.51%.
Fusobacterium nucleatum and Malignant Tumors of the Digestive Tract: A Mechanistic Overview
Authors: Lai Yue , Mi Jun , Feng Qiang
Published: June 2022

Fusobacterium nucleatum (F. nucleatum) is an oral anaerobe that plays a role in several oral diseases. However, F. nucleatum is also found in other tissues of the digestive tract, and several studies have recently reported that the level of F. nucleatum is significantly elevated in malignant tumors of the digestive tract. F. nucleatum is proposed as one of the risk factors in the initiation and progression of digestive tract malignant tumors. In this review, we summarize recent reports on F. nucleatum and its role in digestive tract cancers and evaluate the mechanisms underlying the action of F. nucleatum in digestive tract cancers.
Adhesion of Resin to Lithium Disilicate with Different Surface Treatments before and after Salivary Contamination—An In-Vitro Study
Authors: Harouny Ryan , Hardan Louis , Harouny Elie , Kassis Cynthia , Bourgi Rim , Lukomska-Szymanska Monika , Kharouf Naji , Ball Vincent , Khairallah Carlos
Published: June 2022

The salivary contamination occurring at the try-in procedures of lithium disilicate (LDS) can jeopardize their bond strength. Various laboratory reports have concluded that applying 37% phosphoric acid (H3PO4) could be considered as a predictable way of removing salivary contaminants. An experimental method that consists of sealing the intaglio of the ceramic restorations with a layer of cured adhesive could allow consequent time saving for dental practitioners. It is, besides, necessary to establish an optimal decontamination protocol. Hence, this study aimed to determine the most efficient surface treatment, before and after salivary contamination, by comparing the adhesion between resin and LDS. In order to do so, five groups of ten specimens (n = 10) each underwent the different types of surface treatments before bonding, followed by 2500 cycles in the thermocycler. A shear bond strength (SBS) test was then conducted on a universal testing machine (YLE GmbH Waldstraße Bad König, Germany), followed by a fracture-type analysis on an optical microscope (Olympus BX53, Shinjuku, Tokyo, Japan). Statistical analysis was set with a level of significance of α = 0.05. The surface treatment significantly affected the SBS results. The decontamination with HF (12.59 ± 2.71 MPa) and H3PO4 (13.11 ± 1.03 MPa) obtained the highest values, silanizing only before contamination obtained intermediate values (11.74 ± 3.49 MPa), and silanizing both before and after the salivary contamination (10.41 ± 2.75 MPa) along with applying a bonding agent before contamination (9.65 ± 1.99 MPa) resulted in the lowest values. In conclusion, H3PO4 proved to be efficient, thus, allowing the practitioner to avoid the clinical use of HF; it can, therefore, be considered as a valid alternative. Presilanization and resilanization of specimens, along with applying a bonding agent before contamination, did not yield satisfying results.
Cortisol as an Independent Predictor of Unfavorable Outcomes in Hospitalized COVID-19 Patients
Authors: Świątkowska-Stodulska Renata , Berlińska Agata , Puchalska-Reglińska Ewelina
Published: June 2022

Most cases of COVID-19 are non-severe, but some patients require urgent hospital care. In the past, it has been established that adrenal hyperactivity predicts poorer prognosis in severely ill patients. We wanted to verify if cortisol levels can be tied to clinical outcomes and the degree of inflammation in hospitalized COVID-19 patients. We recruited 180 adult patients with PCR-confirmed COVID-19. The group was divided into smaller subgroups based on the glucocorticoid treatment status; the subgroups were evaluated in three separate time points. The assessment involved hormonal function (cortisol, ACTH), inflammatory markers, and occurrence of the pre-selected endpoints (death, hospitalization ≥10 days, non-invasive ventilation or high-flow oxygenation, mechanical ventilation, vasopressors). In the evaluated group, 121 patients showed signs of abnormal adrenal function. There was a clear correlation between cortisol and IL-6 concentrations in all three time points regardless of glucocorticoid treatment. A total of 71.1% of patients displaying abnormal cortisol production met the preselected endpoints. Our analysis showed that a cutoff cortisol concentration prognosing endpoint occurrence could be set at 15.45 μg/dL for patients not treated with glucocorticoids. Cortisol concentration can be seen as an independent prognostic factor for unfavorable outcomes in selected adults hospitalized with COVID-19.
Ubiquitin-Specific Protease 6 n-Terminal-like Protein (USP6NL) and the Epidermal Growth Factor Receptor (EGFR) Signaling Axis Regulates Ubiquitin-Mediated DNA Repair and Temozolomide-Resistance in Glioblastoma
Authors: Su I-Chang , Su Yu-Kai , Chuang Hao-Yu , Yadav Vijesh Kumar , Setiawan Syahru Agung , Fong Iat-Hang , Yeh Chi-Tai , Huang Hui-Chuan , Lin Chien-Min
Published: June 2022

Glioblastoma multiforme (GBM) is the most malignant glioma, with a 30–60% epidermal growth factor receptor (EGFR) mutation. This mutation is associated with unrestricted cell growth and increases the possibility of cancer invasion. Patients with EGFR-mutated GBM often develop resistance to the available treatment modalities and higher recurrence rates. The drug resistance observed is associated with multiple genetic or epigenetic factors. The ubiquitin-specific protease 6 N-terminal-like protein (USP6NL) is a GTPase-activating protein that functions as a deubiquitinating enzyme and regulates endocytosis and signal transduction. It is highly expressed in many cancer types and may promote the growth and proliferation of cancer cells. We hypothesized that USP6NL affects GBM chemoresistance and tumorigenesis, and that its inhibition may be a novel therapeutic strategy for GBM treatment. The USP6NL level, together with EGFR expression in human GBM tissue samples and cell lines associated with therapy resistance, tumor growth, and cancer invasion, were investigated. Its pivotal roles and potential mechanism in modulating tumor growth, and the key mechanism associated with therapy resistance of GBM cells, were studied, both in vitro and in vivo. Herein, we found that deubiquitinase USP6NL and growth factor receptor EGFR were strongly associated with the oncogenicity and resistance of GBM, both in vitro and in vivo, toward temozolomide, as evidenced by enhanced migration, invasion, and acquisition of a highly invasive and drug-resistant phenotype by the GBM cells. Furthermore, abrogation of USP6NL reversed the properties of GBM cells and resensitized them toward temozolomide by enhancing autophagy and reducing the DNA damage repair response. Our results provide novel insights into the probable mechanism through which USP6NL/EGFR signaling might suppress the anticancer therapeutic response, induce cancer invasiveness, and facilitate reduced sensitivity to temozolomide treatment in GBM in an autolysosome-dependent manner. Therefore, controlling the USP6NL may offer an alternative, but efficient, therapeutic strategy for targeting and eradicating otherwise resistant and recurrent phenotypes of aggressive GBM cells.
Optogenetically Engineered Neurons Differentiated from Human SH-SY5Y Cells Survived and Expressed ChR2 in 3D Hydrogel
Authors: Lee Si-Yuen , George Julian , Nagel David , Ye Hua , Seymour Leonard
Published: June 2022

The cases of brain degenerative disease will rise as the human population ages. Current treatments have a transient effect and lack an investigative system that is physiologically relevant for testing. There is evidence suggesting optogenetic stimulation is a potential strategy; however, an in vitro disease and optogenetic model requires a three-dimensional microenvironment. Alginate is a promising material for tissue and optogenetic engineering. Although it is bioinert, alginate hydrogel is transparent and therefore allows optical penetration for stimulation. In this study, alginate was functionalized with arginine-glycine-aspartate acid (RGD) to serve as a 3D platform for encapsulation of human SH-SY5Y cells, which were optogenetically modified and characterized. The RGD-alginate hydrogels were tested for swelling and degradation. Prior to encapsulation, the cells were assessed for neuronal expression and optical-stimulation response. The results showed that RGD-alginate possessed a consistent swelling ratio of 18% on day 7, and degradation remained between 3.7–5% throughout 14 days. Optogenetically modified SH-SY5Y cells were highly viable (>85%) after lentiviral transduction and neuronal differentiation. The cells demonstrated properties of functional neurons, developing beta III tubulin (TuJ1)-positive long neurites, forming neural networks, and expressing vGlut2. Action potentials were produced upon optical stimulation. The neurons derived from human SH-SY5Y cells were successfully genetically modified and encapsulated; they survived and expressed ChR2 in an RGD-alginate hydrogel system.
CoCl2-Mimicked Endothelial Cell Hypoxia Induces Nucleotide Depletion and Functional Impairment That Is Reversed by Nucleotide Precursors
Authors: Kutryb-Zajac Barbara , Kawecka Ada , Braczko Alicja , Franczak Marika , Slominska Ewa M. , Giovannoni Roberto , Smolenski Ryszard T.
Published: June 2022

Chronic hypoxia drives vascular dysfunction by various mechanisms, including changes in mitochondrial respiration. Although endothelial cells (ECs) rely predominantly on glycolysis, hypoxia is known to alter oxidative phosphorylation, promote oxidative stress and induce dysfunction in ECs. Our work aimed to analyze the effects of prolonged treatment with hypoxia-mimetic agent CoCl2 on intracellular nucleotide concentration, extracellular nucleotide breakdown, mitochondrial function, and nitric oxide (NO) production in microvascular ECs. Moreover, we investigated how nucleotide precursor supplementation and adenosine deaminase inhibition protected against CoCl2-mediated disturbances. Mouse (H5V) and human (HMEC-1) microvascular ECs were exposed to CoCl2-mimicked hypoxia for 24 h in the presence of nucleotide precursors: adenine and ribose, and adenosine deaminase inhibitor, 2′deoxycoformycin. CoCl2 treatment decreased NO production by ECs, depleted intracellular ATP concentration, and increased extracellular nucleotide and adenosine catabolism in both H5V and HMEC-1 cell lines. Diminished intracellular ATP level was the effect of disturbed mitochondrial phosphorylation, while nucleotide precursors effectively restored the ATP pool via the salvage pathway and improved endothelial function under CoCl2 treatment. Endothelial protective effects of adenine and ribose were further enhanced by adenosine deaminase inhibition, that increased adenosine concentration. This work points to a novel strategy for protection of hypoxic ECs by replenishing the adenine nucleotide pool and promoting adenosine signaling.
Detection of Oxytetracycline Using an Electrochemical Label-Free Aptamer-Based Biosensor
Authors: Akbarzadeh Sanaz , Khajehsharifi Habibollah , Hajihosseini Saeedeh
Published: June 2022

One of the most effective ways to detect and measure antibiotics is to detect their biomarkers. The best biomarker for the control and detection of oxytetracycline (OTC) is the OTC-specific aptamer. In this study, a novel, rapid, and label-free aptamer-based electrochemical biosensor (electrochemical aptasensor) was designed for OTC determination based on a newly synthesized nanocomposite including multi-walled carbon nanotubes (MWCNTs), gold nanoparticles (AuNPs), reduced graphene oxide (rGO), and chitosan (CS), as well as nanosheets to modify a glassy carbon electrode, which extremely enhanced electrical conductivity and increased the electrode surface to bind well with the amine-terminated OTC-specific aptamer through self-assembly. The (MWCNTs-AuNPs/CS-AuNPs/rGO-AuNPs) nanocomposite modified electrode was synthesized using a layer- by-layer modification method which had the highest efficiency for better aptamer stabilization. Differential pulse voltammetry (DPV), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM) techniques were used to investigate and evaluate the electrochemical properties and importance of the synthesized nanocomposite in different steps. The designed aptasensor was very sensitive for measuring the OTC content of milk samples, and the results were compared with those of our previously published paper. Based on the calibration curve, the detection limit was 30.0 pM, and the linear range was 1.00–540 nM for OTC. The repeatability and reproducibility of the aptasensor were obtained for 10.0 nM of OTC with a relative standard deviation (RSD%) of 2.39% and 4.01%, respectively, which were not affected by the coexistence of similar derivatives. The measurement in real samples with the recovery range of 93.5% to 98.76% shows that this aptasensor with a low detection limit and wide linear range can be a good tool for detecting OTC.