bluuebunny/crossref_metadata_2025 huggingface dataset:
Economic Comparison of Satellite, Plane and UAV-Acquired NDVI Images for Site-Specific Nitrogen Application: Observations from Italy
Authors: Sozzi Marco , Kayad Ahmed , Gobbo Stefano , Cogato Alessia , Sartori Luigi , Marinello Francesco
Published: October 2021

Defining the most profitable remote sensing platforms is a difficult decision-making process, as it requires agronomic and economic considerations. In this paper, the price and profitability of three levels of remote sensing platforms were evaluated to define a decision-making process. Prices of satellite, plane and UAV-acquired vegetation indices were collected in Italy during 2020 and compared to the economic benefits resulting from variable rate nitrogen application, according to a bibliographic meta-analysis performed on grains. The quality comparison of these three technologies was performed considering the error propagation in the NDVI formula. The errors of the single bands were used to assess the optical properties of the sensors. Results showed that medium-resolution satellite data with good optical properties could be profitably used for variable rate nitrogen applications starting from 2.5 hectares, in case of medium resolution with good optical properties. High-resolution satellites with lower optical quality were profitable starting from 13.2 hectares, while very high-resolution satellites with good optical properties could be profitably used starting from 76.8 hectares. Plane-acquired images, which have good optical properties, were profitable starting from 66.4 hectares. Additionally, a reference model for satellite image price is proposed.
Modelling Agroforestry’s Contributions to People—A Review of Available Models
Authors: Kraft Philipp , Rezaei Ehsan Eyshi , Breuer Lutz , Ewert Frank , Große-Stoltenberg André , Kleinebecker Till , Seserman Diana-Maria , Nendel Claas
Published: October 2021

Climate change, increasing environmental pollution, continuous loss of biodiversity, and a growing human population with increasing food demand, threaten the functioning of agro-ecosystems and their contribution to people and society. Agroforestry systems promise a number of benefits to enhance nature’s contributions to people. There are a wide range of agroforestry systems implemented representing different levels of establishment across the globe. This range and the long time periods for the establishment of these systems make empirical assessments of impacts on ecosystem functions difficult. In this study we investigate how simulation models can help to assess and predict the role of agroforestry in nature’s contributions. The review of existing models to simulate agroforestry systems reveals that most models predict mainly biomass production and yield. Regulating ecosystem services are mostly considered as a means for the assessment of yield only. Generic agroecosystem models with agroforestry extensions provide a broader scope, but the interaction between trees and crops is often addressed in a simplistic way. The application of existing models for agroforestry systems is particularly hindered by issues related to code structure, licences or availability. Therefore, we call for a community effort to connect existing agroforestry models with ecosystem effect models towards an open-source, multi-effect agroforestry modelling framework.
Changes in Bacterial Soil Biota under Cultivation of Crops near a Municipal Landfill Site
Authors: Ropek Dariusz Roman , Frączek Krzysztof
Published: October 2021

The study aimed to evaluate the changes in the quantitative composition of a soil bacterial community near a municipal waste landfill, and attempted to use a bacteriological coefficient to assess the degree of soil degradation. The research was carried out near a landfill site located in southern Poland. Soil samples were collected from plots on which spring wheat, field bean and potato were cultivated. Microbiological analyses included the determination of the total number of bacteria in active and dormant (sporulating) stages. The highest ratio of sporulating bacteria in relation to vegetative bacteria was found in the reclaimed sector of the landfill site. The proposed bacteriological indicator of soil quality (i.e., the ratio of the number of sporulating bacteria to the number of vegetative forms) seems to be a good index for the assessment of soil quality near the landfill site.
Hybrid Machine Learning Models for Forecasting Surgical Case Volumes at a Hospital
Authors: Aravazhi Agaraoli
Published: October 2021

Recent developments in machine learning and deep learning have led to the use of multiple algorithms to make better predictions. Surgical units in hospitals allocate their resources for day surgeries based on the number of elective patients, which is mostly disrupted by emergency surgeries. Sixteen different models were constructed for this comparative study, including four simple and twelve hybrid models for predicting the demand for endocrinology, gastroenterology, vascular, urology, and pediatric surgical units. The four simple models used were seasonal autoregressive integrated moving average (SARIMA), support vector regression (SVR), multilayer perceptron (MLP), and long short-term memory (LSTM). The twelve hybrid models used were a combination of any two of the above-mentioned simple models, namely, SARIMA–SVR, SVR–SARIMA, SARIMA–MLP, MLP–SARIMA, SARIMA–LSTM, LSTM–SARIMA, SVR–MLP, MLP–SVR, SVR–LSTM, LSTM–SVR, MLP–LSTM, and LSTM–MLP. Data from the period 2012–2018 were used to build and test the models for each surgical unit. The results indicated that, in some cases, the simple LSTM model outperformed the others while, in other cases, there was a need for hybrid models. This shows that surgical units are unique in nature and need separate models for predicting their corresponding surgical volumes.
Prevalence and Novel Genotypes Identification of Enterocytozoon bieneusi in Dairy Cattle in Yunnan Province, China
Authors: Song Hai-Yang , Wang Kai-Sheng , Yang Jian-Fa , Mao Hua-Ming , Pu Li-Hua , Zou Yang , Ma Jun , Zhu Xing-Quan , Zou Feng-Cai , He Jun-Jun
Published: October 2021

Enterocytozoon bieneusi is a fungus-like protist parasite that can cause diarrhea and enteric diseases. The infection of E. bieneusi has been reported in many host species, including cattle and humans. However, information on prevalence and genotype distribution of E. bieneusi in dairy cattle in Yunnan province in China is still absent. In this study, 490 Holstein Cows and 351 dairy buffalo fecal samples were collected from three regions in Yunnan province, China. By using nest-PCR that targets the internal transcribed spacer (ITS), we found that the prevalence of E. bieneusi was 0.59% (5/841). DNA sequence analysis showed that five E. bieneusi genotypes were identified in this study, including two novel genotypes, YNDCEB-90 and YNDCEB-174, and three known genotypes (I, J, BEB4). Phylogenetic analysis revealed that two novel genotypes, YNDCEB-90 and YNDCEB-174, were clustered into Group 1, representing the zoonotic potential. The remaining genotypes I, J, and BEB4, which are the most frequent genotypes of E. bieneusi infection in cattle and lead to E. bieneusi infection in humans, belonged to Group 2. Although the lower prevalence of E. bieneusi was detected in dairy cattle in Yunnan province, it indicates that dairy cattle should be considered to be one of the potential hosts for transmitting E. bieneusi to humans. These findings are important for the development of effective prevention strategies for microsporidiosis.
Application of Methods to Assess Animal Welfare and Suffering Caused by Infectious Diseases in Cattle and Swine Populations
Authors: Nielsen Søren Saxmose , Houe Hans , Denwood Matthew , Nielsen Liza Rosenbaum , Forkman Björn , Otten Nina Dam , Agger Jens Frederik
Published: October 2021

Control of infectious diseases in livestock has often been motivated by food safety concerns and the economic impact on livestock production. However, diseases may also affect animal welfare. We present an approach to quantify the effect of five infectious diseases on animal welfare in cattle (three diseases) and pigs (two diseases). We grouped clinical manifestations that often occur together into lists of clinical entities for each disease based on literature reviews, and subsequently estimated “suffering scores” based on an aggregation of duration, frequency, and severity. The duration and severity were based on literature reviews and expert knowledge elicitation, while frequency was based mainly on estimates from the literature. The resulting suffering scores were compared to scores from common welfare hazards found under Danish conditions. Most notably, the suffering scores for cattle diseases were ranked as: bovine viral diarrhoea and infection with Mycobacterium avium subsp. paratuberculosis > infectious bovine rhinotracheitis, and for pigs as: porcine respiratory and reproductive syndrome > Aujeszky’s disease. The approach has limitations due to the limited data available in literature and uncertainties associated with expert knowledge, but it can provide decision makers with a tool to quantify the impact of infections on animal welfare given these uncertainties.
Use of Infrared Thermography during Ejaculation Process and Its Link with Semen Quality and Freezability in Dogs
Authors: Tekin Koray , İnanç Muhammed Enes , Özen Doğukan , Cil Beste , Olğaç Kemal Tuna , Yılmaz Burak , Taşdemir Umut , Tuncer Pürhan Barbaros , Büyükleblebici Serhat , Daşkın Ali , Uysal Ongun , Stelletta Calogero
Published: October 2021

This study aimed to describe the thermal variation of external reproductive tracts during ejaculation in relation to sperm quality in dogs. Forty-six adult fertile dogs were monitored using a thermal camera before, during and after the semen collection, taking into account penile and scrotal temperatures as reproductive thermal patterns while eye and perianal temperatures were recorded as complementary thermal patterns of behavioral response. The parameters were classified depending on age (≤4 years and >4 years), body weight (BW) (≤75 kg and >75 kg), sperm concentration (CON) (≤300 million and >300 million), total testicular volume (TTV) (≤600 cm3 and >600 cm3) and total ejaculation time (TET) (≤800 s and >800 s) of the animals from which semen was collected successfully. Heavier males (p < 0.05) that have more consistent testicles (p < 0.01) as well as quicker ejaculate responders (p < 0.001) and lower scrotal temperature had better semen (Δ motility) freezability. The lower eye temperature prior to the ejaculation (p < 0.01), lower scrotal temperature following ejaculation (p < 0.01), and conversely, higher penile temperature during the ejaculation (p < 0.001) had a higher sperm concentration. Furthermore, the sperm freezability was negatively correlated with total ejaculation time (r = −0.39, p < 0.05) and sperm abnormalities were lower in the ejaculate of dogs having a higher temperature of the scrotum, bulbus and penis. In conclusion, infrared monitoring throughout semen collection in dogs can provide information on behavioral reactions during human manipulation, as well as semen quality and testicular functionality.
Perches as Cooling Devices for Reducing Heat Stress in Caged Laying Hens: A Review
Authors: Hu Jiaying , Xiong Yijie , Gates Richard S. , Cheng Heng-Wei
Published: October 2021

Heat stress is one of the most detrimental environmental challenges affecting the biological process and the related production performance of farm animals, especially in poultry. Commercial laying hens have been bred (selected) for high egg production, resulting in increased sensitivity to heat stress due to breeding-linked metabolic heat production. In addition, laying hens are prone to heat stress due to their inadequate species-specific cooling mechanisms resulting in low heat tolerance. In addition, hens have no sweat glands and feathering covers almost their entire body to minimize body heat loss. The poultry industry and scientists are developing cooling methods to prevent or reduce heat stress-caused damage to chicken health, welfare, and economic losses. We have designed and tested a cooling system using perches, in which chilled water (10 °C) circulates through a conventional perch passing through the layer cages to offer the cooling potential to improve hen health, welfare, and performance during acute and chronic periods of heat stress (35 °C). This review summarizes the outcomes of a multi-year study using the designed cooled perch system. The results indicate that conducting heat from perching hens directly onto the cooled perch system efficiently reduces heat stress and related damage in laying hens. It provides a novel strategy: perches, one key furnishment in cage-free and enriched colony facilities, could be modified as cooling devices to improve thermal comfort for hens during hot seasons, especially in the tropical and subtropical regions.
Efficacy of Ceftazidime and Cefepime in the Management of COVID-19 Patients: Single Center Report from Egypt
Authors: Eid Ragaey A. , Elgendy Marwa O. , El-Gendy Ahmed O. , Elgendy Sara O. , Belbahri Lassaad , Sayed Ahmed M. , Rateb Mostafa E.
Published: October 2021

The purpose of this study was to explore the value of using cefepime and ceftazidime in treating patients with COVID-19. A total of 370 (162 males) patients, with RT-PCR-confirmed cases of COVID-19, were included in the study. Out of them, 260 patients were treated with cefepime or ceftazidime, with the addition of steroids to the treatment. Patients were divided into three groups: Group 1: patients treated with cefepime (124 patients); Group 2: patients treated with ceftazidime (136 patients); Group 3 (control group): patients treated according to the WHO guidelines and the Egyptian COVID-19 management protocol (110 patients)/ Each group was classified into three age groups: 18–30, 31–60, and >60 years. The dose of either cefepime or ceftazidime was 1000 mg twice daily for five days. Eight milligrams of dexamethasone were used as the steroidal drug. Careful follow-ups for the patients were carried out. In vitro and in silico Mpro enzyme assays were performed to investigate the antiviral potential of both antibiotics. The mean recovery time for Group 1 was 12 days, for Group 2 was 13 days, and for Group 3 (control) was 19 days. No deaths were recorded, and all patients were recovered without any complications. For Group 1, the recovery time was 10, 12, and 16 days for the age groups 18–30, 30–60, and >60 years, respectively. For Group 2, the recovery time was 11, 13, and 15 days for the age groups 18–30, 30–60, and >60 years, respectively. For Group 3 (control), the recovery time was 15, 16, and 17 days for the age groups 18–30, 30–60, and >60 years, respectively. Both ceftazidime and cefepime showed very good inhibitory activity towards SARS CoV-2′s Mpro, with IC50 values of 1.81 µM and 8.53 µM, respectively. In conclusion, ceftazidime and cefepime are efficient for the management of moderate and severe cases of COVID-19 due to their potential anti-SARS CoV-2 activity and low side effects, and, hence, the currently used complex multidrug treatment protocol can be replaced by the simpler one proposed in this study.
Neuroprotective Activity of Melittin—The Main Component of Bee Venom—Against Oxidative Stress Induced by Aβ25–35 in In Vitro and In Vivo Models
Authors: Nguyen Cong Duc , Lee Gihyun
Published: October 2021

Melittin, a 26-amino acid peptide, is the main component of the venom of four honeybee species and exhibits neuroprotective actions. However, it is unclear how melittin ameliorates neuronal cells in oxidative stress and how it affects memory impairment in an in vivo model. We evaluated the neuroprotective effect of melittin on Aβ25–35-induced neuro-oxidative stress in both in vitro HT22 cells and in vivo animal model. Melittin effectively protected against HT22 cell viability and significantly deregulated the Aβ25–35-induced overproduction of intracellular reactive oxygen species. Western blot analysis showed that melittin suppressed cell apoptosis and regulated Bax/Bcl-2 ratio, as well as the expression of proapoptotic related factors: Apoptosis-inducing factor (AIF), Calpain, Cytochrome c (CytoC), Cleaved caspase-3 (Cleacas3). Additionally, melittin enhanced the antioxidant defense pathway by regulating the nuclear translocation of nuclear factor erythroid 2-like 2 (Nrf2) thus upregulated the production of the heme oxygenase-1 (HO-1), a major cellular antioxidant enzyme combating neuronal oxidative stress. Furthermore, melittin treatment activated the Tropomyosin-related kinase receptor B (TrkB)/cAMP Response Element-Binding (CREB)/Brain-derived neurotrophic factor (BDNF), contributing to neuronal neurogenesis, and regulating the normal function of synapses in the brain. In our in vivo experiment, melittin was shown to enhance the depleted learning and memory ability, a novel finding. A mouse model with cognitive deficits induced by Aβ25–35 intracerebroventricular injection was used. Melittin had dose-dependently enhanced neural-disrupted animal behavior and enhanced neurogenesis in the dentate gyrus hippocampal region. Further analysis of mouse brain tissue and serum confirmed that melittin enhanced oxidant–antioxidant balance, cholinergic system activity, and intercellular neurotrophic factors regulation, which were all negatively altered by Aβ25–35. Our study shows that melittin exerts antioxidant and neuroprotective actions against neural oxidative stress. Melittin can be a potential therapeutic agent for neurodegenerative disorders.
Selecting Remote Driving Locations for Latency Sensitive Reliable Tele-Operation
Authors: Zulqarnain Syed Qamar , Lee Sanghwan
Published: October 2021

These days, autonomous vehicles (AVs) technology has been improved dramatically. However, even though the AVs require no human intervention in most situations, AVs may fail in certain situations. In such cases, it is desirable that humans can operate the vehicle manually to recover from a failure situation through remote driving. Furthermore, we believe that remote driving can enhance the current transportation system in various ways. In this paper, we consider a revolutionary transportation platform, where all the vehicles in an area are controlled by some remote controllers or drivers so that transportation can be performed in a more efficient way. For example, road capacity can be effectively utilized and fuel efficiency can be increased by centralized remote control. However, one of the biggest challenges in such remote driving is the communication latency between the remote driver and the vehicle. Thus, selecting appropriate locations of the remote drivers is very important to avoid any type of safety problem that might happen due to large communication latency. Furthermore, the selection should reflect the traffic situation created by multiple vehicles in an area. To tackle these challenges, in this paper, we propose several algorithms that select remote drivers’ locations for a given transportation schedules of multiple vehicles. We consider two objectives in this system and evaluate the performance of the proposed algorithms through simulations. The results show that the proposed algorithms perform better than some baseline algorithms.
Disease-Free Survival after Breast Conservation Therapy vs. Mastectomy of Patients with T1/2 Breast Cancer and No Lymph Node Metastases: Our Experience
Authors: Fanizzi Annarita , Ressa Maurizio Cosmo , Gatta Gianluca , Cristofaro Cristian , De Santis Valerio , Didonna Vittorio , Diotaiuti Sergio , Forgia Daniele La , Petruzzellis Nicole , Tamborra Pasquale , Lorusso Vito , Massafra Raffaella
Published: October 2021

Several retrospective analyses of large amounts of contemporary data have shown the superiority of breast conservative surgery (BCS) over mastectomy carried out in the early stage of breast cancer. The characteristics of the patients and cancers that are most likely to benefit from BCS remain unclear. In our work, we analyzed the disease-free survival (DFS) of a cohort of patients treated with BCS or mastectomy between 1995 and 2018 in our institute with pT1-2, pN0, or cM0 breast cancer. The DFS curves of patients treated with both mastectomy and quadrantectomy were compared in the different subsamples with respect to the clinical and histopathological characteristics. We identified 188 eligible patients treated with BCS and 64 patients treated with mastectomy. DFS was not found to be statistically higher in patients treated with BCS compared to those treated with mastectomy, who achieved a 5-year DFS of 89.9% vs. 81.3% and a 10-year DFS of 78.9% vs. 79.3%, respectively. No significant differences were detected for the DFS curves when patients were differentiated by the type of surgical treatment received, age, and the tumor histological characteristics. We verified a p-value just above the 10% significance threshold for patients with tumor dimensions between 20 mm and 50 mm and molecular sub-type Luminal B. In our experience, treatment with mastectomy is not associated with improved DFS compared to treatment with BCS in women with early-stage tumors.
Non-Invasive Physical Plasma Enhances the Membrane Permeability to Low Molecular Weight Compounds and Subsequently Leads to the Loss of Cellular ATP and the Devitalization of Epithelial Cancer Cells
Authors: Sander Caroline , Nitsch Andreas , Erb Holger H. H. , Egger Eva K. , Haralambiev Lyubomir , Eggers Benedikt , Kramer Franz-Josef , Weiss Martin , Mustea Alexander , Stope Matthias B.
Published: October 2021

Non-invasive physical plasma (NIPP) achieves biomedical effects primarily through the formation of reactive oxygen and nitrogen species. In clinical use, these species interact with cells of the treated tissue, affecting the cytoplasmic membrane first. The present study investigated the permeability of the cytoplasmic membrane of breast cancer cells with different fluorescent dyes after NIPP treatment and determined the subsequent effects on cell viability. After NIPP treatment and the associated formation of reactive oxygen species, low molecular weight compounds were able to pass through the cytoplasmic membrane in both directions to a higher extent. Consequently, a loss of cellular ATP into the extracellular space was induced. Due to these limitations in cell physiology, apoptosis was induced in the cancer cells and the entire cell population exhibited decreased cell growth. It can be concluded that NIPP treatment disturbs the biochemical functionality of the cytoplasmic membrane of cancer cells, which massively impairs their viability. This observation opens a vast application horizon of NIPP therapy to treat precancerous and malignant diseases beyond breast cancer therapy.
Brewing with 10% and 20% Malted Lentils—Trials on Laboratory and Pilot Scales
Authors: Trummer Jonas , Watson Hellen , De Clippeleer Jessika , Poreda Aleksander
Published: October 2021

Lentils, a popular foodstuff worldwide, are gaining more interest for their use in alternative diets. In addition, we are observing an ever-growing demand for new raw materials in the malting and brewing industry and an overall rising interest in a low-gluten lifestyle. Therefore, in this study, malt was produced from green lentils and used in both laboratory- and pilot-scale brewing trials. Malted lentils were used as 10% and 20% adjuncts at the laboratory scale, following the Congress mash procedure, and the most important parameters (e.g., filtration time, pH, color, extract, fermentability) of the wort and beer samples were analyzed with a special focus on the concentrations of metal ions (Mg2+, Ca2+, Zn2+, Fe) in wort. The production of beer with lentil malt as an adjunct was then scaled up to 1 hl, and several beer parameters were analyzed, including the gluten content and foam stability. The results showed that the gluten content was decreased by circa 35% and foam stability was enhanced by approximately 6% when adding 20% lentil malt. Furthermore, the use of lentil malt reduced the filtration time by up to 17%. A trained panel evaluated the sensorial qualities of the produced beers. Overall, the use of green lentil malt shows promising results for its potential use in brewing.
Model and Knowledge Representation for the Reuse of Design Process Knowledge Supporting Design Automation in Mass Customization
Authors: Dworschak Fabian , Kügler Patricia , Schleich Benjamin , Wartzack Sandro
Published: October 2021

Mass customization aims to meet individual requirements and, therefore, is one way to attract and retain customers—a key challenge in the design industry. The increase in design automation has offered new opportunities to design customized products at high speed in a way that is cost equivalent to mass production. Design automation is built upon the reuse of product and process knowledge. Ontologies have proven to be a feasible, highly aggregated knowledge representation in engineering design. While product and process knowledge from other lifecycle phases are represented in multiple approaches, the design process of the product as well as the adaption process of product variants is missing, causing breakpoints or additional iterations in design automation. Therefore, suitable knowledge representation tailored to design automation is still missing. Accordingly, this contribution proposes a novel knowledge representation approach to enable design automation for mass customization. Methodically, this novel approach uses semantic enrichment of CAD environments to automatically deduce information about a design task, design rationale, and design process represented by a formal ontology. The integration of the design process significantly differentiates the approach from previous ones. The feasibility of the approach is demonstrated by a bike crank customization process.
Classification between Elderly Voices and Young Voices Using an Efficient Combination of Deep Learning Classifiers and Various Parameters
Authors: Lee Ji-Yeoun
Published: October 2021

The objective of this research was to develop deep learning classifiers and various parameters that provide an accurate and objective system for classifying elderly and young voice signals. This work focused on deep learning methods, such as feedforward neural network (FNN) and convolutional neural network (CNN), for the detection of elderly voice signals using mel-frequency cepstral coefficients (MFCCs) and linear prediction cepstrum coefficients (LPCCs), skewness, as well as kurtosis parameters. In total, 126 subjects (63 elderly and 63 young) were obtained from the Saarbruecken voice database. The highest performance of 93.75% appeared when the skewness was added to the MFCC and MFCC delta parameters, although the fusion of the skewness and kurtosis parameters had a positive effect on the overall accuracy of the classification. The results of this study also revealed that the performance of FNN was higher than that of CNN. Most parameters estimated from male data samples demonstrated good performance in terms of gender. Rather than using mixed female and male data, this work recommends the development of separate systems that represent the best performance through each optimized parameter using data from independent male and female samples.
Modelling of a Non-Transferred Plasma Torch Used for Nano-Silica Powders Production
Authors: AlShunaifi Ibrahim A. , Elaissi Samira , Ghiloufi Imed , Alterary Seham S. , Alharbi Ahmed A.
Published: October 2021

In this study, a two-dimensional numerical model was developed to simulate operation conditions in the non-transferred plasma torch, used to synthesis nanosilica powder. The turbulent magnetohydrodynamic model was presented to predict the nitrogen plasma flow and heat transfer characteristics inside and outside the plasma torch. The continuity, momentum, energy, current continuity equations, and the turbulence model were expressed in cylindrical coordinates and numerically solved by COMSOL Multiphysics software with a finite element method. The operation conditions of the mass flow rate of ionized gas ranging from 78 sccm to 240 sccm and the current varying between 50 A to 200 A were systematically analyzed. The variation in the electrothermal efficiency with the gas flow rate, the plasma current, and the enthalpy was also reported. The results revealed that the increase in working current lead to a raise in the effective electric power and then an increase in the distribution of plasma velocity and temperature. The efficiency of the torch was found to be between 36% and 75%. The plasma jet exited the nozzle torch with a larger fast and hot core diameter with increasing current. The numerical results showed good correlation and good trends with the experimental measurement. This study allowed us to obtain more efficient control of the process conditions and a better optimization of this process in terms of the production rate and primary particle size. X-ray diffraction (XRD) and transmission electron microscopy (TEM) were used to characterize the primary nanosilica powder that was experimentally collected. The arc plasma method enabled us to produce a spherical silicon ultra-fine powder of about 20 nm in diameter.
Preparation, Characterization and Evaluation of Guar Films Impregnated with Relaxing Peptide Loaded into Chitosan Microparticles
Authors: Batista Patrícia , Castro Pedro M. , Madureira Ana Raquel , Sarmento Bruno , Pintado Manuela
Published: October 2021

Background: Biological molecules such as therapeutic proteins and peptides have provided ample opportunities for further improvements in health and wellbeing. However, such biomacromolecules face stability challenges regarding premature processing and digestion, whenever any enteric delivery route is considered. Hence, oral guar films entrapping peptide-containing chitosan microparticles have been developed as a new delivery system to carry and protect therapeutic relaxing peptides. Results: The composite films were characterized regarding physicochemical properties using FTIR as well as peptide encapsulation efficiency, cytotoxicity, and peptide permeability through buccal and intestinal cell-based models. The composite films with 0.18% (w/w) of peptide demonstrated ca. 0.63% of peptide loading efficiency into the chitosan microparticles and to be safe for buccal and intestinal epithelial cells up to 24 h of contact. The permeability assays through TR146 cells to mimic buccal mucosa epithelium and Caco-2/HT29-MTX to mimic duodenum conditions showed that the incorporation of peptide chitosan microparticles into guar-gum oral films offered a faster permeability, when compared with the free peptide and peptide-chitosan-microparticles-loaded chitosan. Conclusion: This study suggests that peptide-loaded chitosan microparticles into guar-gum oral films might be a promising carrier for enhancement of the buccal and intestinal absorption of a relaxing peptide.
Requirements Analysis in Disruptive Engineering Solutions Using the Paradigm of Living Systems
Authors: Brad Emilia , Brad Stelian
Published: October 2021

A particular characteristic of disruptive products is in reengineering advanced technologies for addressing the needs of low-end consumers and/or non-consumers, to transform them into new consumers. This requires a lean co-creative analysis of requirements with all stakeholders involved. Even if a theory encourages the continuous connection of designers and users throughout the design lifecycle for agile adaptation of requirements to the new experiences of users by intersecting them with various versions of the prototype, the rigid budget and time allocated to the design project require novel approaches to clarify the right vectors of product-evolution from the very early design stages of the project lifecycle—allowing agile approaches to fine-tune the set of requirements. In this context, an analysis process of requirements that uses a constructor inspired by living systems is introduced in this paper. This constructor identifies gaps in requirement formulation and indicates areas where improvements must be undertaken. The method is applied in the case of a new cybersecurity software solution that targets micro and small companies.
Controllable Synthesis of 1, 3, 5-tris (1H-benzo[d]imidazole-2-yl) Benzene-Based MOFs
Authors: Gaikwad Sanjit , Cheedarala Ravi Kumar , Gaikwad Ranjit , Kim Soonho , Han Sangil
Published: October 2021

The growing interest in metal–organic frameworks (MOFs) in both industrial and scientific circles has increased in the last twenty years, owing to their crystallinity, structural versatility, and controlled porosity. In this study, we present three novel MOFs obtained from the 1, 3, 5-tris (1H-benzo[d]imidazole-2-yl) benzene (TIBM) organic linker. The formed TIBM crystal powders were characterized by scanning electron microscopy (SEM) to estimate the morphology of the particles, powder X-ray diffraction (XRD) to confirm the crystal structure, Brunauer–Emmett–Teller (BET) method for structural analysis, and thermogravimetric measurements to examine the thermal stability. The TIBM-Cu MOF showed excellent CO2 (3.60 mmol/g) adsorption capacity at 1 bar and 298 K, because of the open Cu site, compared to TIBM-Cr (1.6 mmol/g) and TIBM-Al (2.1 mmol/g). Additionally, due to the high porosity (0.3–1.5 nm), TIBM-Cu MOF showed a considerable CO2/N2 selectivity (53) compared to TIBM-Al (35) and TIBM-Cr (10).
Characterization of Transitivity in L-Tolerance Spaces by Convergence and Closure
Authors: Jäger Gunther , Ahsanullah T. M. G.
Published: October 2021

We show that the category of quantale-valued tolerance spaces is isomorphic to a category of quantale-valued convergence spaces. We define suitable quantale-valued closure functions and use them to characterize transitivity axioms. Furthermore, transitivity is characterized by convergence and diagonal axioms. Quantale-valued tolerance relations compatible with group structures are also characterized by convergence and it is shown that they are transitive.
Deficiencies in Root Canal Fillings Subsequent to Adaptive Instrumentation of Oval Canals
Authors: Pawar Ajinkya M. , Bhardwaj Anuj , Banga Kulvinder S. , Singh Gurdeep , Kfir Anda , Luke Alexander Maniangat , Dinata Vialyne , Wahjuningrun Dian Agustin
Published: October 2021

The purpose of this study was to explore the influence of instrumentation and the potential for debris deposition using XP-endo shaper plus (XP-SP) and full-sequence SAF (F-SAF) on the adaption of thermoplastic root canal fillings in oval canals. Following the manufacturer’s instructions, ninety human permanent mandibular incisors with a single oval canal 6 mm from the apex (verified using pre-operative CBCT scanning) were instrumented with XP-SP and F-SAF. Obtura III Max apparatus was used for root canal obturation without the use of a root canal sealer. The roots were then sectioned 6 mm from the apex and examined with a digital stereomicroscope at x25 magnification to assess the root canal fillings. The F-SAF was associated with a significantly higher (p < 0.01) percentage of entire adaptation of the root fillings (76%) compared to the XP-SP (57%). Furthermore, the XP-SP group was also associated with higher (p < 0.01) defective obturation with debris at 17% and with voids at 26%. However, the F-SAF had lower percentages of defective obturations (7% with debris and 17% with voids). The quality of obturation of oval canals instrumented using full-sequence SAF was better.
Comparative Analysis of Biological Characteristics among P0 Proteins from Different Brassica Yellows Virus Genotypes
Authors: Zhang Xiao-Yan , Li Yuan-Yuan , Wang Ying , Li Da-Wei , Yu Jia-Lin , Han Cheng-Gui
Published: October 2021

Brassica yellows virus (BrYV) is a tentative species of the genus Polerovirus, which has at least three genotypes (A, B, and C) in China. The P0 protein of BrYV-A (P0BrA) has been identified as a viral suppressor of RNA silencing (VSR), which can also induce cell death in infiltrated Nicotiana benthamiana leaves. In this study, we demonstrated that the cell death induced by P0BrA was accompanied by the accumulation of reactive oxygen species (ROS) and increased Pathogenesis-related protein genes-1 (PR1) expression. Meanwhile, this cell death phenotype was delayed by salicylic acid (SA) pretreatment. Biological function comparison of the three P0 proteins showed that transiently expressed P0BrB or P0BrC induced a significantly delayed and milder cell death response compared with P0BrA. However, like P0BrA, they also suppressed local and systemic RNA silencing. Six residues of P0BrA essential for inducing cell death were identified by comparative analysis and amino acid substitution assay. We also show that all three BrYV genotypes have synergistic interactions with pea enation mosaic virus 2 (PEMV 2) in N. benthamiana. This study provides theoretical guidance for controlling the viral disease caused by poleroviruses in the future.
Repurposing Disulfiram for Targeting of Glioblastoma Stem Cells: An In Vitro Study
Authors: Zirjacks Lisa , Stransky Nicolai , Klumpp Lukas , Prause Lukas , Eckert Franziska , Zips Daniel , Schleicher Sabine , Handgretinger Rupert , Huber Stephan M. , Ganser Katrin
Published: October 2021

Mesenchymal glioblastoma stem cells (GSCs), a subpopulation in glioblastoma that are responsible for therapy resistance and tumor spreading in the brain, reportedly upregulate aldehyde dehydrogenase isoform-1A3 (ALDH1A3) which can be inhibited by disulfiram (DSF), an FDA-approved drug formerly prescribed in alcohol use disorder. Reportedly, DSF in combination with Cu2+ ions exerts multiple tumoricidal, chemo- and radio-therapy-sensitizing effects in several tumor entities. The present study aimed to quantify these DSF effects in glioblastoma stem cells in vitro, regarding dependence on ALDH1A3 expression. To this end, two patient-derived GSC cultures with differing ALDH1A3 expression were pretreated (in the presence of CuSO4, 100 nM) with DSF (0 or 100 nM) and the DNA-alkylating agent temozolomide (0 or 30 µM) and then cells were irradiated with a single dose of 0–8 Gy. As read-outs, cell cycle distribution and clonogenic survival were determined by flow cytometry and limited dilution assay, respectively. As a result, DSF modulated cell cycle distribution in both GSC cultures and dramatically decreased clonogenic survival independently of ALDH1A3 expression. This effect was additive to the impairment of clonogenic survival by radiation, but not associated with radiosensitization. Of note, cotreatment with temozolomide blunted the DSF inhibition of clonogenic survival. In conclusion, DSF targets GSCs independent of ALDH1A3 expression, suggesting a therapeutic efficacy also in glioblastomas with low mesenchymal GSC populations. As temozolomide somehow antagonized the DSF effects, strategies for future combination of DSF with the adjuvant standard therapy (fractionated radiotherapy and concomitant temozolomide chemotherapy followed by temozolomide maintenance therapy) are not supported by the present study.
Traditional Microscopic Techniques Employed in Dental Adhesion Research—Applications and Protocols of Specimen Preparation
Authors: Nawrocka Agnieszka , Piwonski Ireneusz , Sauro Salvatore , Porcelli Annalisa , Hardan Louis , Lukomska-Szymanska Monika
Published: October 2021

Microscopy is a traditional method to perform ex vivo/in vitro dental research. Contemporary microscopic techniques offer the opportunity to observe dental tissues and materials up to nanoscale level. The aim of this paper was to perform a literature review on four microscopic methods, which are widely employed in dental studies concerning the evaluation of resin-dental adhesive interfaces—confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM). The literature search was performed using digital databases: PubMed, Web of Science and Scopus. On the basis of key words relevant to the topic and established eligibility criteria, finally 84 papers were included in the review. Presented microscopic techniques differ in their principle of operation and require specific protocols for specimen preparation. With regard to adhesion studies, microscopy assists in the description of several elements involved in adhesive bonding, as well as in the assessment of the condition of enamel surface and the most appropriate etching procedures. There are several factors determining the quality of the interaction between the substrates which could be recognized and a potential for further implementation of microscopic techniques in dental research could be recognized, especially when these techniques are used simultaneously or combined with spectroscopic methods. Through such microscopy techniques it is possible to provide clinically relevant conclusions and recommendations, which can be easily introduced for enamel-safe bonding and bonding protocols, as well as optimal pretreatments in dentine preparation.
Non-Enzymatic Detection of Glucose in Neutral Solution Using PBS-Treated Electrodeposited Copper-Nickel Electrodes
Authors: Goodnight Lindsey , Butler Derrick , Xia Tunan , Ebrahimi Aida
Published: October 2021

Transition metals have been explored extensively for non-enzymatic electrochemical detection of glucose. However, to enable glucose oxidation, the majority of reports require highly alkaline electrolytes which can be damaging to the sensors and hazardous to handle. In this work, we developed a non-enzymatic sensor for detection of glucose in near-neutral solution based on copper-nickel electrodes which are electrochemically modified in phosphate-buffered saline (PBS). Nickel and copper were deposited using chronopotentiometry, followed by a two-step annealing process in air (Step 1: at room temperature and Step 2: at 150 °C) and electrochemical stabilization in PBS. Morphology and chemical composition of the electrodes were characterized using scanning electron microscopy and energy-dispersive X-ray spectroscopy. Cyclic voltammetry was used to measure oxidation reaction of glucose in sodium sulfate (100 mM, pH 6.4). The PBS-Cu-Ni working electrodes enabled detection of glucose with a limit of detection (LOD) of 4.2 nM, a dynamic response from 5 nM to 20 mM, and sensitivity of 5.47 ± 0.45 μA cm−2/log10(mole.L−1) at an applied potential of 0.2 V. In addition to the ultralow LOD, the sensors are selective toward glucose in the presence of physiologically relevant concentrations of ascorbic acid and uric acid spiked in artificial saliva. The optimized PBS-Cu-Ni electrodes demonstrate better stability after seven days storage in ambient compared to the Cu-Ni electrodes without PBS treatment.
Enzyme (Single and Multiple) and Nanozyme Biosensors: Recent Developments and Their Novel Applications in the Water-Food-Health Nexus
Authors: Alvarado-Ramírez Lynette , Rostro-Alanis Magdalena , Rodríguez-Rodríguez José , Sosa-Hernández Juan Eduardo , Melchor-Martínez Elda M. , Iqbal Hafiz M. N. , Parra-Saldívar Roberto
Published: October 2021

The use of sensors in critical areas for human development such as water, food, and health has increased in recent decades. When the sensor uses biological recognition, it is known as a biosensor. Nowadays, the development of biosensors has been increased due to the need for reliable, fast, and sensitive techniques for the detection of multiple analytes. In recent years, with the advancement in nanotechnology within biocatalysis, enzyme-based biosensors have been emerging as reliable, sensitive, and selectively tools. A wide variety of enzyme biosensors has been developed by detecting multiple analytes. In this way, together with technological advances in areas such as biotechnology and materials sciences, different modalities of biosensors have been developed, such as bi-enzymatic biosensors and nanozyme biosensors. Furthermore, the use of more than one enzyme within the same detection system leads to bi-enzymatic biosensors or multi-enzyme sensors. The development and synthesis of new materials with enzyme-like properties have been growing, giving rise to nanozymes, considered a promising tool in the biosensor field due to their multiple advantages. In this review, general views and a comparison describing the advantages and disadvantages of each enzyme-based biosensor modality, their possible trends and the principal reported applications will be presented.
Interactional Effects of Multidimensional Perfectionism and Cognitive Emotion Regulation Strategies on Eating Disorder Symptoms in Female College Students
Authors: Tng Germaine Y. Q. , Yang Hwajin
Published: October 2021

Given the inconclusive findings regarding the relation between perfectionism and eating disorder symptoms, it is important that we determine whether this relation is modulated by emotion dysregulation, which is a prominent risk factor for eating disorders. We sought to identify specific cognitive emotion regulatory strategies—rumination, self-blame, and catastrophizing—that interact with multidimensional perfectionism to shape eating disorder symptoms (i.e., shape, weight, eating concerns, and dietary restraint). Using latent moderated structural equation modeling, we analyzed data from 167 healthy young female adults. We found that only rumination significantly moderated the relation between socially prescribed perfectionism and eating disorder symptoms. However, this was not observed for self-oriented perfectionism or other regulatory strategies. These findings held true when a host of covariates were controlled for. Our findings underscore the crucial role of rumination, a modifiable emotion regulatory strategy, in augmenting the relation between socially prescribed perfectionism and eating disorder symptoms in young women.
Effects of Reducing Agent on the Activity of PtRu/Carbon Black Anode Catalyst of Direct Methanol Fuel Cell
Authors: Chen Yu-Wen , Chen Han-Gen
Published: October 2021

A series of PtRu/carbon black catalysts were prepared by means of deposition-precipitation and reduced by various reducing agents. NaBH4, HCHO and NaH2PO2, respectively, were used as the reduction agents. Some of the samples were reduced by various amounts of NaH2PO2 to investigate the effects of P/Pt ratios on the characteristics and activity of the catalyst. These catalysts were characterized by X-ray diffraction and transmission electron microscopy. The components of these catalysts were detected by X-ray fluorescence, X-ray photoelectron microscopy, and extended X-ray absorption of fine structures (EXAFS). The methanol oxidation ability of the catalysts was tested by cyclic voltammetry measurement. The results show that NaH2PO2 could effectively reduce the particle size of PtRu metal. It can suppress the growth of metal particles. In addition, the P/Pt ratio is crucial. The catalyst reduced by NaH2PO2 with a P/Pt ratio of 1.2 had the highest activity among all catalysts. It had the higher Pt and Ru metal contents and smaller metal particle size than the other catalysts. Its activity was 253.12 A/g, which is higher that than the commercial catalyst (Johnson Matthey H10100, 251.32 A/g).