bluuebunny/crossref_metadata_2025 huggingface dataset:
Aspectos agronômicos e bromatológicos de leguminosas forrageiras no nordeste brasileiro.
Authors: Teixeira V. I. , Dubeux J. C. B. , Santos M. V. F. , Lira M. de A. , Lira M. de A. , Silva H. M. S.
Published: October 2019

O trabalho objetivou avaliar aspectos agronômicos e bromatológicos de nove leguminosas forrageiras herbáceas na zona da Mata Seca de Pernambuco. As leguminosas estudadas foram: Calopogonium mucunoides Desv, Clitoria ternatea L., Desmodium heterocarpon (L.) DC. subsp. ovalifolium (Prain) Ohashi cv. Itabela, Arachis pintoi Krap e Greg. cv. Amarillo, Pueraria phaseoloides (Roxb.) Benth, Stylosanthes guianensis (Aubl.) Sw. cv. Bandeirante, cv. Cook e cv. Mineirão e Stylosanthes macrocephala Ferreira e Sousa Costa cv. Pioneiro. Foram avaliadas as seguintes variáveis: cobertura do solo, altura da planta, produção de matéria verde, stand, relação folha/caule, produção de sementes, teores de fibra em detergente neutro (FDN), fibra em detergente ácido (FDA), hemicelulose, celulose, lignina, proteína bruta (PB) e matéria mineral (MM) das folhas e caules. Foram realizados quatro cortes, sendo utilizada intensidade de 0 cm para o primeiro corte e 5 cm para os demais. O delineamento foi blocos casualizados com três repetições. A maior produção de MV acumulada foi de 45,6 t/ha/432 dias apresentada pela C. ternatea que também apresentou um dos maiores teores de PB, tanto para a folha (27,4%) quanto para o caule (11,5%). Aos 43 dias após o transplantio, o C. mucunoides foi a leguminosa que apresentou a maior porcentagem de solo coberto. Durante o período experimental (432 dias) não foi observado florescimento dos S. guianensis, enquanto que C. mucunoides, C. ternatea e S. macrocephala apresentaram maior potencial de produção de sementes. A. pintoi, C. ternatea e C. mucunoides mostraram grande potencial para produção de forragem nesta região, com a C. ternatea apresentando os resultados mais promissores quanto à composição química.
Comparison of meat composition and sensory values of two different strains of broiler chickens.
Authors: Sogunle O. M. , Egbeyale L. T. , Alajo O. A. , Adeleye O. O. , Fafiolu A. O. , Onunkwor O. B. , Adegbite J. A. , Fanimo A. O.
Published: October 2019

Este studio fue diseñado para comparar la composición química y calidad de las piezas de la canal de 99 broilers de las líneas Arbor Acre y Marshal MY respectivamente, en la Teaching and Research Farm, University of Agriculture, Abeokuta, Nigeria. En el día 56 del experimento, 6 aves por tratamiento fueron sacrificadas humanitariamente para el análisis y evaluación de la canal. Las muestras de carne de los músculos de la pechuga y muslos fueron analizadas para determinar la composición proximal. Los datos obtenidos fueron sometidos a la prueba t, a nivel de 5% de significación. Los resultados de la composición proximal (p
Forrageamento de Apis mellifera l. Em Oiticica (Licania rigida).
Authors: Silva R. A. , Fernandes D. , Lucena Bezerra L. , Costa Silva W. S. , Barreto de Lima A.
Published: October 2019

Com o objetivo de observar a freqüência de visitas de Apis mellifera L. em flores de oticica, verificando ainda o tipo de coleta realizada pelas abelhas ao longo do dia, esta pesquisa foi conduzida no Campus IV do Centro de Ciências Humanas e Agrárias da Universidade Estadual da Paraíba, localizado no município de Catolé do Rocha-PB. Para se determinar o número das visitas, o contagem foi feito no período de 07:00 as 17:00 horas, a cada dez minutos, durante seis dias. As abelhas forrageiam na oiticica néctar, apresentando um maior número de visitas nas flores localizadas à sombra e nas que receberam menor impacto dos ventos.
Composición del fluido folicular de Alpaca (Vicugna pacos) en diferentes estadios de desarrollo.
Authors: Pacheco J. I. , Coila P.
Published: October 2019

El conocimiento de la composición del fluido folicular es necesario como indicativo de ciertas necesidades nutricionales del ovocito, lo cual ayudaría a la formulación de medios de cultivo de los gametos; el objetivo del presente trabajo fue determinar la cantidad de proteínas totales, albúmina, glucosa, lípidos totales y colesteroles en el fluido de folículos secundarios y terciarios. Las muestras fueron colectadas en matadero de alpacas y congeladas. Se determinó la composición mediante la técnica de espectrofotometría, los resultados indican las siguientes cantidades: proteína total (g/dl) 8,26 y 7,26; albúmina (g/dl) 4,75 y 5,29; glucosa (g/l) 3,56 y 3,31; lípidos totales (g/l) 0,3815 y 0,2775; colesterol (g/dl) 0,1039 y 0,09375 en folículos secundarios (7 mm) respectivamente. Estos resultados indican que no existen grandes diferencias entre ambos grupos por lo que el fluido de folículos secundarios también puede usarse en el enriquecimiento de medios de cultivos para gametos en esta especie.
Influência dos níveis energéticos e protéicos em rações de poedeiras leves em manaus.
Authors: Carioca S. T. , Guimarães Cruz F. G. , Matos P. G. J. , Maquiné L. C. , Chagas E. O. , Oliveira S. C. , Santos Filho A. B.
Published: October 2019

O objetivo deste trabalho foi avaliar a influência de diferentes níveis de energia e proteína, sobre o desempenho e qualidade do ovo de poedeiras leves em Manaus-AM, Brasil. O experimento teve duração de 140 dias divididos em cinco períodos de 28 dias. Foram utilizadas 162 aves da linhagem Lohmann LSL com 38 semanas de idade alojadas em 27 gaiolas.O delineamento experimental utilizado foi o inteiramente casualizado em um fatorial 3x3: três níveis de energia (2700, 2800 e 2900 kcal EM/kg) e três de proteína bruta (15, 16 e 17%) com 3 repetições de seis aves por parcela. Os níveis de energia e proteína influenciaram (p0,05) da interação dos fatores nas variáveis analisadas. Concluí-se que rações com 17% PB e 2700 kcal EM/kg apresentaram melhor conversão alimentar e 17% PB e 2900 kcal EM/kg proporcionaram maior produção de ovos, peso da gema, massa do ovo e peso da casca.
Níveis de cálcio e avaliação óssea e de ovos de avestruzes reprodutoras.
Authors: Almeida Paz I. C. L. , Mendes A. A. , Balog A. , Martins M. R. F. B. , Almeida I. C. L. , Fernandes B. C. S. , Milbradt E. L. , Vulcano L. C. , Komiyama C. M. , Cardoso K. F. G.
Published: October 2019

Realizaram-se análises da densidade óssea em tíbias e fêmures de 6 casais de avestruzes reprodutoras com idade entre 5 a 7 anos, alimentados durante 8 semanas, 3 casais com ração de reprodução (T1), e 3 casais com ração de manutenção (T2). Verificou-se que a produção de ovos não foi afetada pelas dietas, assim como a qualidade da casca dos ovos. No entanto a qualidade dos ossos foi pior para as aves do T2.
Características do pelame de cabras saanen e pardo alpina criadas em ambiente tropical.
Authors: Aiura A. L. O. , Aiura F. S. , Silva R. G.
Published: October 2019

Realizou-se um estudo das seguintes características morfológicas do pelame: espessura da capa (E), comprimentos médios dos pêlos (C), número de pêlos por área (N), ângulo de inclinação dos pêlos em relação à superfície (A) e diâmetro médio dos pêlos (D) em 532 cabras das raças Saanen e Pardo Alpina criadas em ambiente tropical As médias e erros-padrões dessas características foram: E= 6,04±0,08 mm; C= 33,17±0,24 mm; N= 191,50±4,67 pêlos.cm-2; A= 10,67±0,15 graus e D= 0,203±0,0013 mm.
Calidad nutritiva de Brachiaria humidicola con fertilización orgánica e inorgánica en suelos ácidos.
Authors: Jiménez O. M. M. , Granados L. , Oliva J. , Quiroz J. , Barrón M.
Published: October 2019

El objetivo de este estudio fue evaluar el efecto de la aplicación de fertilizante orgánico e inorgánico sobre la calidad nutritiva de Brachiaria humidicola, en tres épocas climáticas del año a los 21, 28 y 35 días de crecimiento. El estudio se condujo sobre una pradera localizada en suelos ácidos. Los efectos principales estudiados fueron: época climática (E), sequía, lluvias e invierno; tipo de fertilizante (TF), sin fertilizante, fertilizante inorgánico (150-60-00 NPK) y orgánico (15-4,8-8,4 NPK); edad de corte 21, 28 y 35 días y sus interacciones. Se utilizaron tres réplicas por tratamiento. Las variables estudiadas fueron: proteína bruta (PB), digestibilidad in situ de la materia seca (DIMS), fibra detergente neutro (FDN) y digestibilidad de la FDN (DFDN). Los efectos principales estudiados y sus interacciones afectaron (p
Efeitos da bioestimulação sobre a performance reprodutiva em bovinos de corte.
Authors: Menezes L. M. , Brauner C. C. , Pimentel M. A.
Published: October 2019

As baixas taxas de natalidade são a principal causa do baixo rendimento produtivo e econômico da pecuária de cria no Brasil. Nas últimas décadas, diversas técnicas de manejo como suplementação nutricional de fêmeas, desmame precoce, e utilização de hormônios foram objeto de pesquisa visando aumentar os índices reprodutivos dos rebanhos. A bioestimulação ou efeito macho é definida como o efeito estimulatório via feromônio genital ou outros sinais externos menos compreendidos causados por um macho ou fêmea androgenizada sobre o estro, puberdade ou ovulação de uma fêmea da mesma espécie. É um técnica de manejo que pode ser utilizada para reduzir o período de anestro pós parto em vacas de corte. Em novilhas, o efeito macho sobre a puberdade não é bem esclarecido. Entretanto, há evidências que diferentes períodos de exposição, tipo e formas de bioestimulação podem ser importantes para aumentar o percentual de novilhas cíclicas. O objetivo desta revisão é elencar formas de bioestimulação, tipos, métodos e técnicas utilizadas, fatores e resultados que afetam a eficiência reprodutiva em bovinos de corte.
Rasgos de rendimiento de dos crías cruzadas de cerdos de cepa en cama profunda
Authors: Guimarães G. G. , Murata L. S. , Mcmanus C. , Santana A. P. , Reckziegel G. C. , Amancio A. S. , Hijo R. M. J. , Nephew A. J. F.
Published: October 2019

Este experimento se realizó para evaluar el rendimiento de los cerdos criados en una camada.480 animales se distribuyeron en un diseño de bloques al azar en una disposición factorial 2 x 2 (cruces x sexos), con tres repeticiones de cuarenta animales por unidad experimental. El grosor del tocino (ET) entre las costillas 10 y 11 y la altura de P2 y el área del ojo del lomo (AOL) se evaluaron mediante ultrasonido en tiempo real y características de rendimiento. Se verificó que hubo un efecto sexual, donde los machos mostraron resultados más altos para el consumo de alimento semanal (CS) y diario (CD), sin embargo, la conversión (CA) y la eficiencia alimenticia (AE) presentaron resultados más bajos en comparación con las hembras. No hubo efecto sobre el aumento de peso diario (GPD) y el peso final (PF) entre hombres y mujeres.
Caracterización socioeconómica y productiva de unidades caprinas familiares en la mixteca poblana.
Authors: Hernández J. E. , Franco F. J. , Villarreal O. A. , Camacho J. C. , Pedraza R. M.
Published: October 2019

El objetivo del trabajo fue la caracterización socioeconómica y productiva de 15 unidades caprinas de tipo familiar en el municipio de Piaxtla, Puebla, mediante la realización de encuestas, que incluyeron características sociales, económicas y zootécnicas. De las dieciséis variables utilizadas el análisis de componentes principales selecciona nueve. Los índices de calidad de vida tanto en oportunidades de empleo, educación, salud, vivienda, alimentación balanceada, salario, deporte y cultura son precarios; lo que ha ocasionado durante varias décadas una fuerte corriente de migración de los jóvenes a USA.
Comportamento ingestivo de vacas alimentadas com cana-de-açúcar e diferentes níveis de concentrado.
Authors: Costa L. T. , Silva F. F. , Veloso C. M. , Platillo A. J. V. , Rocha Neto A. L. , Bonomo P. , Mendes F. B. L. , Oliveira J. S. , Azevêdo S. T. , Silva V. L.
Published: October 2019

Foram estudados os efeitos de diferentes níveis de suplementação de vacas leiteiras alimentadas à base de cana-de-açúcar sobre o comportamento ingestivo destes animais. Os níveis de suplementação concentrada foram definidos pelo balanceamento das dietas para conter nutrientes suficientes para mantença e produção de 6, 9, 12 e 15 kg.dia-1 de leite. O experimento foi conduzido na fazenda Paulistinha, na cidade de Macarani-BA, no período de julho a setembro de 2006. Foram utilizadas 16 vacas mestiças Holandês x Zebu distribuídas em um delineamento inteiramente ao acaso, onde estes animais foram observados por dois dias consecutivos e feito uma média dos resultados obtidos para cada animal, chegando a 32 observações e oito repetições por tratamento. Os tempos despendidos com alimentação, ruminação e ócio, o tempo de mastigação total, o número de bolos ruminais, o número de mastigações merícicas por dia e o tempo de ruminação por bolo não diferiram entre os tratamentos (p>0,05), enquanto que para o número de mastigações merícicas por bolo apresentou um comportamento quadrático e para as eficiências de alimentação e ruminação houve um acréscimo linear com o aumento do nível de concentrado.
Efectos de la suplementación con cobre sobre los sistemas oxidativo y antioxidante en broilers.
Authors: Ajuwon O. R. , Idowu O. M. O. , Afolabi S. A. , Kehinde B. O. , Oguntola O. O. , Olatunbosun K. O.
Published: October 2019

El estudio fue diseñado para estudiar los efectos in vivo de la suplementación dietética con cobre sobre los sistemas oxidativos y antioxidantes de los eritrocitos y el hígado de broilers. Las aves fueron sometidas a una dieta comercial (grupo control, n= 20) o suplementada con 250 mg CuSO4/kg dieta (grupo experimental, n= 20) durante 6 semanas. En los eritrocitos y tejido hepático, se evaluó, empleando técnicas consolidadas, la sobrecarga de cobre, la peroxidación oxidativa de los lípidos (LPO), concentración de glutatión (GSH), actividad de los enzimas antioxidantes, super oxido dismutasa (SOD) y catalasa (CAT). La exposición oral al cobre, durante 6 semanas, aumentó (p
Efecto del uso de una enzima y sistema de alimentación sobre productividad en pavos.
Authors: García-López J. C. , Pinos-Rodríguez J. M. , García-Galicia I. A. , Galicia-Juárez G. B. , Gorostiola-Herrera M. L. , Camacho-Escobar M. A.
Published: October 2019

Se evaluó el efecto de una enzima (proteasas, amilasas, celulolasas y galactosidasas) sobre la productividad y calidad de la canal en pavos en confinamiento y semi-confinamiento en la etapa de finalización. Un diseño completamente al azar con arreglo factorial 2 x 2 fue utilizado con 800 pavos White Diamond (nueve semanas de edad, 5084 g peso vivo) a los tratamientos: sistema (confinamiento y semi-confinamiento) y enzima (0 y 0,1% como % DM total de la dieta) para un total de cuatro tratamientos, 5 replicas por tratamiento, 40 pavos por réplica. La ganancia de peso (WG), peso vivo (LW), consumo de alimento (FI), consumo de forraje (FGI) y la calidad de la canal (CY) fueron registrados semanalmente por ocho semanas. Los pavos bajo el sistema de semi-confinamiento tuvieron mayor peso final, ganancia de peso total, ganancia de peso diaria, peso de la canal caliente y fría, así como menor conversión alimenticia que los pavos en el sistema de confinamiento. El porcentaje de la canal fría y caliente fue similar en los pavos en ambos sistemas. La enzima incrementó el peso corporal final, ganancia de peso total, ganancia diaria promedio, peso de la canal caliente y fría, así como también disminuyó la conversión alimenticia en pavos bajo el sistema de semi-confinamiento. El consumo de alimento y el porcentaje de la canal caliente y fría no fueron afectados por las enzimas. La enzima tuvo un efecto benéfico en las características de crecimiento y calidad de la canal en pavos bajo el sistema de semi-confinamiento.
Análise do ambiente para frangos por meio da lógica fuzzy: uma revisão
Authors: Ponciano P. F. , Lopes M. A. , Yanagi Junior T. , Ferraz G. A. S.
Published: October 2019

O objetivo desta revisão foi de discutir a utilização da lógica fuzzy na análise das variáveis que afetam a ambiência de frangos de corte e exemplificar situações bem sucedidas de utilização dessa metodologia na predição das variáveis que afetam o conforto térmico para frangos de corte, além de apresentar a potencialidade dessa metodologia que pode ser usada como suporte à tomada de decisão sobre o controle da climatização dos galpões avícolas, garantindo, assim, melhorias na produção. Dentre as variáveis analisadas por meio da lógica fuzzy, destaca-se o controle da temperatura, umidade relativa, luminosidade, poluentes aéreos e concentração de alguns gases. Esses fatores influenciam diretamente a condição de conforto e bem-estar das aves, afetando sua produtividade. Portanto, é de grande importância uma ferramenta que pode contribuir na tomada de decisão, possibilitando, o controle do ambiente térmico no interior de galpões para produção de frangos.
Sustentabilidad de agroecosistemas.
Authors: Toro-Mujica P. , García A. , Gómez-Castro A. G. , Acero R. , Perea J. , Rodríguez-Estévez V.
Published: October 2019

En este trabajo se revisan, desde la perspectiva de los agroecosistemas, los conceptos de susten-tabilidad reflejados en sus dimensiones: ecológica, social y económica, así como lo relativo al grado de sustitución entre capital natural y humano que se manifiesta en las versiones de la sustentabilidad: débil, que asume la capacidad de sustitución total entre estos recursos, y fuerte que postula que los sistemas naturales son de mayor entidad que los criterios de eficiencia económica. Entre las características principales de sustentabilidad de los agroecosistemas, la resiliencia, asociada a la estabilidad económica y la estabilidad social, debe ser considerada como la desencadenante de la continuidad o discontinuidad de los sistemas. La conveniencia debe ser abordada abandonando los conceptos antropocéntricos y productivistas del corto plazo en beneficio de una concepción holística de los sistemas de producción. Por último, la escala, espacial o temporal, es el factor que permite una cierta adaptación al sistema analizado, modulando la escala temporal a la jerarquización y complejidad de los sistemas agropecuarios y estableciendo como unidad espacial la explotación, sin olvidar la interacción con las actividades de explotaciones adyacentes.
Percursos e Memórias Acadêmicas e Profissionais
Authors: Demetrio Vanio
Published: October 2019

Memorial apresentado no Mestrado em Educação Tecnológica e Profissional
O PROCESSO DE ENSINO E APRENDIZAGEM DA MATEMÁTICA ESCOLAR NA EDUCAÇÃO ESPECIAL: ABORDAGENS E PERSPECTIVAS
Authors: Medeiros Margarete Farias , Oliveira Helen Savi Mondo de , Silveira Katia Elizete , Ribeiro Elizete Maria Possamai , Helfenstein Mara Juliane Woiciechoski , Moraes Ana Maria de , Medeiros Alessandra Olisia , Raimundo Augusto Kurtz
Published: October 2019

O presente artigo tem por finalidade apresentar um relato de experiência sobre a realização do projeto de extensão direcionado a alunos com necessidades específicas da rede pública do município de Sombrio (SC). Foram envolvidos alunos das séries finais do Ensino Fundamental de duas escolas, uma estadual e outra municipal, com metodologias alternativas de ensino, utilizando materiais manipulativos e softwares, procurando atender as dificuldades de aprendizagem da Matemática escolar. O projeto em questão teve como objetivo apresentar possibilidades metodológicas para o ensino da Matemática escolar a alunos com necessidades específicas, bem como propiciar às acadêmicas do Curso Superior de Licenciatura em Matemática uma formação baseada na constante busca por novas metodologias de ensino, no sentido de desafiá-las a rever constantemente suas práticas pedagógicas, dentro de uma postura reflexiva diante das mais diversas realidades em que possam se deparar.  Na realização desta experiência, apontamos como pontos positivos a experiência para as acadêmicas bolsistas, a participação dos alunos, seu interesse e a disponibilidade das escolas envolvidas. Destacamos, ainda, que os alunos envolvidos apresentavam dificuldades de aprendizagem na Matemática escolar, mas com a utilização de materiais manipulativos estes superavam suas dificuldades e conseguiam participar na sala de aula apresentando atenção e concentração nas atividades.
GESTÃO DE RESÍDUOS SÓLIDOS EM UMA EMPRESA SEM FINS LUCRATIVOS
Authors: Vieira Merielin Cristina , Passos Tiago Germano , Maidel Simone
Published: October 2019

O presente artigo aborda de maneira simples e direta a implantação de um sistema de gestão dos resíduos sólidos em uma empresa de pequeno porte, sem fins lucrativos.  O projeto teve como objetivo geral a redução, reutilização e reciclagem de Resíduos Sólidos, bem como a implantação da coleta seletiva, em conformidade com a legislação ambiental e foram utilizados como apoio três objetivos específicos: 1) Evidenciar a necessidade de implantação da Gestão de Resíduos Sólidos na empresa Capri Iate Clube; 2) Definir formas de armazenagem de cada categoria de resíduos e 3) Conscientizar colaboradores e sócios sobre a importância da Gestão de Resíduos Sólidos. Como referência, para o planejamento das ações foram utilizadas as diretrizes da Lei nº 12.305/10, que institui a Política Nacional de Resíduos Sólidos (PNRS). Embora ainda não finalizado, o projeto já alcançou resultados positivos e a simpatia dos sócios e frequentadores da empresa, contribuindo para a conscientização das consequências decorrentes do manejo inadequado dos resíduos sólidos na comunidade e encorajando esforços futuros para completa implantação do projeto na empresa.Palavras chaves: resíduos sólidos; coleta seletiva; responsabilidade ambiental.
O JIU-JITSU NA VIDA DOS ALUNOS DO IFC CAMPUS SANTA ROSA DO SUL: CONTRIBUIÇÕES PARA A FORMAÇÃO INTEGRAL
Authors: Mesquita Junior Paulo Fernando , Silveira Jose Vitor Alves
Published: October 2019

O presente trabalho trata-se de um projeto de extensão realizado, no âmbito do Instituto Federal Catarinense Santa Rosa do Sul (IFC-SRS) com início no ano de 2012, fomentado pelo próprio campus, com o auxílio de alunos voluntários, com foco na oferta de uma oficina esportiva da modalidade de luta Jiu-Jitsu, visando ampliar os espaços que fortalecem e contribuem com o processo educativo promovido pela instituição. As aulas consistem de dois encontros semanais, tendo cada sessão, duração de aproximada de 1 hora e 30 minutos, os quais acontecem no Ginásio Poliesportivo do IFC-SRS. Os encontros são organizados com exercícios de aquecimento e exercícios de preparação física, seguidos de elementos técnicos, como: posições, estrangulamentos, chaves, raspagens, passagens de guarda; e de discussões sobre temas que envolvam a luta. Resultados e conclusão: o Jiu-Jitsu, confirmou-se como um instrumento privilegiado no que se refere à educação integral não só de jovens escolares, mas também como de adultos; interferindo positivamente na vida dos participantes do projeto e no espaço institucional.
Comparison of the metabolites profiles between a Graft Chimera ‘Hongrou Huyou’ ( Citrus changshan-huyou + C. unshiu Marc.) and Two Donors
Authors: Zhang Min , Jing Luyang , Wu Qun , Zhu Kaijie , Ke Fuzhi , Xu Jianguo , Zhao Siqing , Wang Gang , Zhang Chi
Published: October 2019

Background: Chimeras synthesized artificially by grafting are crucial to breeding of perennial woody plants. ‘Hongrou Huyou’ ( Citrus changshan-huyou + C . unshiu Marc.) was a new grafting chimera originated from the junction where a scion Citrus changshan-huyou (“C”) was top-grafted onto a stock Satsuma mandarin ‘Owari’ ( C . unshiu Marc., “O”). The chimera was simplified as OCC because the cell layer constitutions were O for L1 and C for L2 & L3, respectively. In this study, profiles of primary metabolites, volatiles and carotenoids derived from different tissues were investigated between OCC and two donors, aiming to figure out the relationship between layer donor and metabolites.
Results: Comparison of the metabolite profiles showed that the amount and composition of metabolites were different between the peels and the juice sacs, as well as between OCC and two donors. Metabolites (such as violaxanthin and β -cryptoxanthin in carotenoids, germacrene D in volatiles, citric acid and sorbose in primary metabolites) specifically present or absent in the certain tissue were identified in three phenotypes. According to the principal component analysis (PCA), the total metabolites of chimeric peel properties were derived from the donor C, whereas those of chimeric juice sac properties came from the donor O.
Conclusion: Profiles of primary metabolites, volatiles and carotenoids derived from the peels and the juice sacs were systematically compared between OCC and two donors. The content and composition of metabolites differed in tissues as well as between OCC and two donors. Donor dominant metabolite inheritance was dramatic in the different tissues of OCC and basically consistent with the layer origin that the chimeric peels were derived from C and the chimeric juice sacs came from O. These profiles provide potentially chemical markers for genotype differentiation and citrus breeding assessment, more than that, for donor selection during chimera synthesis artificially.
Cloning and Functional Analysis of the FAD2 Gene Family from Desert Shrub Artemisia sphaerocephala
Authors: Miao Xiumei , zhang lijing , Hu Xiaowei , Nan Shuzhen , Chen Xiaolong , Fu Hua
Published: October 2019

Background: Linoleic acid is an important polyunsaturated fatty acid, required for all eukaryotes. Microsomal delta-12 (Δ12) oleate desaturase (FAD2) is a key enzyme for linoleic acid biosynthesis. Desert shrub Artemisia sphaerocephala is rich in linoleic acid, it has a large FAD2 gene family with twenty-six members. The aim of this work is to unveil the difference and potentially functionality of AsFAD2 family members.
Results: Full-length cDNAs of twenty-one AsFAD2 genes were obtained from A. sphaerocephala. The putative polypeptides encoded by AsFAD2 family genes showed a high level of sequence similarity and were relatively conserved during evolution. The motif composition was also relatively conservative. Quantitative real-time PCR analysis revealed that the AsFAD2-1 gene was strongly expressed in developing seeds, which may be closely associated with the high accumulating ability of linoleic acid in A. sphaerocephala seeds. Although different AsFAD2 family members showed diverse response to salt stress, the overall mRNA levels of the AsFAD2 family genes was stable. Transient expression of AsFAD2 genes in the Nicotiana benthamiana leaves revealed that the encoded proteins were all located in the endoplasmic reticulum. Heterologous expression in Saccharomyces cerevisiae suggested that only three AsFAD2 enzymes, AsFAD2-1, -10, and -23, were Δ12 oleate desaturases, which could convert oleic acid to linoleic acid, whereas AsFAD2-1 and AsFAD2-10 could also produce palmitolinoleic acid.
Conclusions: This research reported the cloning, expression studies, subcellular localization and functional identification of the large AsFAD2 gene family. These results should be helpful in understanding fatty acid biosynthesis in A. sphaerocephala, and has the potential to be applied in the study of plant fatty acids traits.
Clinical practice competency and associated factors among graduating nursing students attending at Universities in Amhara region, Ethiopia: Institution based cross-sectional study, 2018
Authors: Getie ፟Addisu , Tsige Yosief , Birhanie Emebet , Getaneh Kenean , Demis Asmamaw
Published: October 2019

Objective To assess clinical practice competency and associated factors among graduating nursing students in universities of Amhara region, Northern Ethiopia. Result This study showed that, 33.6% of the participants were clinically competent and there is slight variation in the clinical competency of participants at different Universities found in Amhara region. Clinical practice competency of students was affected by clinical instructor factors. Students who had got orientation about objective of clinical practice were 2.387 times more likely to be clinically competent [AOR: 2.387; 95% CI (1.011-5.635)]. Students who had clinical instructor who spent enough time for mentoring them during clinical practice had 2.247 times more likely to be clinical competent [AOR: 2.247; 95% CI (1.100-4.593)]. Students who had instructors that follow them while they conduct a procedure were 2.655 times more likely clinically competent [AOR: 2.655; 95% CI (1.294-5.449)]. This study also revealed that clinical practice competency of students was affected by assessment methods factors. Students with clinical practice assessment checklist had 2.663 times higher clinical practice competency [AOR: 2.663; 95% CI (1.324-5.358)]. Regarding staff-student factors, students who were allowed to perform tasks by clinical staffs were 5.858 times more likely to be clinical competent task [AOR: 5.858; 95% CI (2.657-12.916)]
Outbreak of KPC-2 Carbapenem-Resistant Klebsiella pneumoniae ST76 and Carbapenem-Resistant K2 Hypervirulent Klebsiella pneumoniae ST375 Strains in Northeast China: Molecular and Virulent Characteristics
Authors: Su Shanshan , Zhao Yongxin , Yu Lan , Li Chunjiang , Wang Yong , Wang Yuchao , Bao Mingjia , Fu Yu , Zhang Jisheng , Zhang Xiaoli
Published: October 2019

Background Carbapenem-resistant hypervirulent Klebsiella pneumoniae strains have recently come into existence worldwide; however, researchers in northeast China are not aware of their clinical features and molecular characteristics. Methods Here, the molecular and virulent characteristics of 44 carbapenem-resistant K. pneumoniae (CRKP) isolates collected from January 2015 to December 2017 were studied. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were carried out to define the clonal relatedness among the isolates. PCR and capsular serotyping of the virulence-associated genes, as well as biofilm formation and serum complement-mediated killing assays, were employed to determine the virulent potential. The genomic features and associated mobile genetic elements of JmsCRE57 were detected by whole genome sequencing. Results The only positive isolate was JmsCRE57, which belonged to the ST375 serotype K2 that expressed uge, mrkD, fimH, kpn, aerobactin and rmpA virulence-associated genes and showed strong biofilm formation and serum sensitivity. Sequencing results showed that the JmsCRE57 genome mainly consisted of a circular chromosome, three antibiotic resistance plasmids and a virulent plasmid. The antibiotic resistance plasmid expressing blaKPC-2, blaCTX-M-15, aph(3'')-Ib, aph(6)-Id, qnrB1, aac(3)-IIa, aac(6')-Ib-cr, blaOXA-1, blaTEM-1B, catB4, sul2, dfrA14 and blaSHV-99. The virulent plasmid belonged to the IncHI1B group, which is mainly composed of mucoid phenotype genes and siderophore-associated genes. The remaining CRKP strains that expressed uge, fimH, mrkD and kpn virulence-associated genes were not successfully typed. Conclusion Our results provide new insights on the epidemiology of carbapenem-resistant K2 hypervirulent K. pneumoniae ST375 and CRKP ST76 strains in northeast China, which may help control their future outbreaks.
Later Onset of Hypertension Is Associated with Higher Risk of Dementia in Mild Cognitive Impairment
Authors: qin hongyun , Hu chengping , Wang ling , Guo yi , Cao Zhicheng , Zhao xudong , Zhu binggen
Published: October 2019

Objective

To investigate the correlation between hypertension development and the progression of MCI for middle aged and elderly people.

Methods

A population-based longitudinal cognition survey of people aged 55+ was conducted. The hypertension onset age was estimated by self-reported information and medical insurance card records. In order to study the effect of later onset of hypertension on dementia, the incidence of dementia was compared between the two groups.

Results

A total of 277 hypertensive mild cognitive impairment (MCI) participants without dementia were followed for 6 years. Overall, 56 MCI participants (20.22%) progressed to dementia (MCIp). The proportion of MCIp participants in the old-age onset hypertension group (≥ 65 years) was higher than that of middle-age onset one (27.0% vs 15.4%; X2 = 5.538, P = 0.019). And in the old-age onset hypertension group, the proportion of MCIp without diabetes mellitus was higher than those with diabetes mellitus (24.7% vs 12.6%; X2 = 5.321, P = 0.021), and the proportion of MCIp with increased pulse pressure was higher than those without increased pulse pressure (33.3% vs 15.4%; X2 = 3.902, P = 0.048). However, the multivariate logistic regression analysis showed that older age was the only risk for MCIp (OR=0.732, p=0.029).

Conclusions

These results suggest that, individuals with later onset hypertension may have greater cognition decline, even in cases with blood pressure maintained at 130/80 mmHg with antihypertensive management.
Familial autosomal recessive bestrophinopathy: identification of a novel variant in BEST1 gene and the specific metabolomic profile
Authors: Ye Panpan , Xu Jia , Luo Yueqiu , Su Zhitao , yao ke
Published: October 2019

BackgroundAutosomal recessive bestrophinopathy (ARB) is a retinal degenerative disorder caused by BEST1 mutations with autosomal recessive inheritance. We aim to map a comprehensive genomic and metabolic profile of a consanguineous Chinese family with ARB.MethodsOphthalmic examinations were performed on affected patients with ARB. Target capture sequencing was performed to screen causative mutations in 256 known retinal disease genes and Sanger sequencing were used for verification. A UHPLC-MS/MS metabolomic analysis was performed to explore the disease-associated metabolic feature.ResultsAffected patients from this family were characterized by low vision, the presence of subretinal fluid, macular edema, and hyperopia with coincidental angle closure. DNA sequencing identified a novel missense mutation in BEST1 gene (chr11:61725867G>A) of the proband. Sanger sequencing further confirmed the mutation. The blood metabolic profiles were highly similar among all family members probably due to the same life style, habitats and genomic background. However, ARB patients presented significant deregulation of metabolites such as Citric acid, L-Threonic acid, Eicosapentaenoic acid.Conclusions
We identified a novel disease-associated variant in BEST1 gene as well as a disease-specific metabolic feature in familial ARB. The findings improved the understanding of mechanisms of ARB and provided a potential therapeutic strategy with application of metabolomics.
Spontaneous malignant transformation of ovarian surface epithelial cells correlates with EMT alteration and stemness acquisition
Authors: Li Bingyan , Zhang Hemei , Guo Yi , Liu Lizhi , Hou Yongfeng , Wang Ping , Jiang Fei , Zhang Zengli , Hei Tom K
Published: October 2019

Background

In order to complete the physiological function of postovulatory repair during repeated ovulation, the ovarian surface epithelium (OSE) not only has to undergo epithelial-mesenchymal transition (EMT), but also possess the properties of somatic stem-like cells. However, there is no evidence to indicate that both EMT alteration and stemness acquisition are linked to epithelial ovarian carcinomas.Methods

In this study, we established a cell model of spontaneous oncogenic transformation of mouse OSE (MOSE). The cell proliferation was assessed using clonogenic survival and soft agar. The self-renewal of cancer stem-like cells (CSCs) was determined by spheroid culture. CD44 + /CD117 + cells were analyzed using flow cytometer. The PCR array was used to determine the EMT-related mRNA level. Expression of pan-keratin, vimentin, E-cadherin, Snail1 and Slug were detected using western-blotting and immunofluorescences, respectively. The tumorigenesis were monitored by limiting dilution assay in vitro and in vivo .Results

Based on morphological change, chromosomal number and proliferating ability, we defined three sequential stages of transformed cells as early, intermediate and late MOSE cells, respectively. We found that MOSE cells had dual characteristics of not only epithelial but mesenchymal nature as well. Over time, MOSE cells spontaneously developed characteristics of malignant cells and generated tumor nodules expressing both Pan-keratin and Vimentin. Furthermore, we found that the neoplastic transformation of MOSE was accompanied by continuous EMT-inducing signals including Snail1 and Slug. Concurrently, the increase of CD44 + /CD117 + cells and their self-renewal ability were associated with the progression of spontaneous neoplastic transformation of MOSE cells in vitro. Conclusion

These results indicated that both EMT alteration and stemness acquisition were closely correlated with the spontaneous malignant progression of MOSE cells. Our findings provide new insights into the future to combat epithelial ovarian carcinomas.
WITHDRAWN: Successful implementation of an Enhanced Recovery After Surgery (ERAS) protocol reduces nausea and vomiting in patients after infratentorial craniotomy: a randomized controlled trial
Authors: Lu Dan , Wang Yuan , Zhao Tianzhi , Liu Bolin , Ye Lin , Zhao Lanfu , Zhao Binfang , Li Mingjuan , Ma Lin , Li Zhengmin , Niu Jiangtao , Lv Wenhai , Zhang Yufu , Zheng Tao , Xue Yafei , Chen Lei , Chen Long , Sun Xude , Gao Guodong , Chen Bo , He Shiming
Published: October 2019

Objectives: Infratentorial craniotomy patients have a high incidence of postoperative nausea and vomiting (PONV). Enhanced Recovery After Surgery (ERAS) protocols have been shown in multiple surgical disciplines to improve outcomes, including reduced PONV. However, very few studies have described the application of ERAS to infratentorial craniotomy. The aim of this study was to examine whether our ERAS protocol for infratentorial craniotomy could improve PONV.Methods: We implemented an evidence-based, multimodal ERAS protocol for patients undergoing infratentorial craniotomy. A total of 105 patients who underwent infratentorial craniotomy were randomized into either the ERAS group (n = 50) or the control group (n = 55). Primary outcomes were the incidence of vomiting, nausea score, and use of rescue antiemetic during the first 72 h after surgery. Secondary outcomes included postoperative anxiety level, sleep quality, and complications.Results: Over the entire 72 h post-craniotomy observation period, the cumulative incidence of vomiting was significantly lower in the ERAS group than in the control group. Meanwhile, the incidence of vomiting was significantly lower in the ERAS group on postoperative days (PODs) 2 and 3. Notably, the proportion of patients with mild nausea (VAS 0-4) was higher in the ERAS group as compared to the control group on PODs 2 or 3. Additionally, the postoperative anxiety level and quality of sleep were significantly better in the ERAS group.Conclusion: Successful implementation of our ERAS protocol in infratentorial craniotomy patients could attenuate postoperative anxiety, improve sleep quality, and reduce the incidence of PONV, without increasing the rate of postoperative complications.
Arabidopsis MLK3, a Plant-specific Casein Kinase 1, Negatively Regulated Flowering and Phosphorylated Histone H3 in vivo
Authors: Wang Zhen , Kang Junmei , Jia Shangang , Zhang Tiejun , Wu Zhihai , Yu Renjie , Yang Qingchuan
Published: October 2019

Background: Casein kinase 1 (CK1) family members are highly conserved serine/threonine kinase present in most eukaryotes with multiple biological functions. Arabidopsis MUT9-like kinases ( MLKs ) belong to a clade CK1 specific to the plant kingdom and have been implicated collectively in modulating flowering related processes. Three of the four MLKs ( MLK1/2/4 ) have been characterized, however, little is known about MLK3 , the most divergent MLKs. Results: We demonstrated that compared with wild type, mlk3 , a truncated MLK3 , flowered slightly early under long day conditions and ectopic expression of MLK3 rescued the morphological defects of mlk3 , indicating that MLK3 negatively regulates flowering. GA 3 application accelerated flowering of both wild type and mlk3 , suggesting that mlk3 had normal GA response. The recombinant MLK3-GFP was localized in the nucleus exclusively. In vitro kinase assay revealed that the nuclear protein MLK3 phosphorylated histone 3 at threonine 3 (H3T3ph). Mutation of a conserved catalytic residue (Lysine 175) abolished the kinase activity and resulted in failure to complement the early flowering phenotype of mlk3 . Interestingly, the global level of H3T3 phosphorylation in mlk3 did not differ significantly from wild type, suggesting the redundant roles of MLKs in flowering regulation. The transcriptomic analysis demonstrated that 425 genes significantly altered expression level in mlk3 relative to wild type. The mlk3 mlk4 double mutant generated by crossing mlk3 with mlk4 , a loss-of-function mutant of MLK4 showing late flowering, flowered between the two parental lines, suggesting that MLK3 played an antagonistic role to MLK4 in plant transition to flowering. Conclusions: A serine/threonine kinase encoding gene MLK3 is a casein kinase 1 specific to the plant species and represses flowering slightly. MLK3 located in nucleus catalyzes the phosphorylation of histone H3 at threonine 3 in vitro and an intact lysine residue (K175) is indispensible for the kinase activity. This study sheds new light on the delicate control of flowering by the plant-specific CK1 in Arabidopsis.
Characteristics of nocardiosis patients with different immune status from a Chinese tertiary general hospital during 8-year period
Authors: Huang Lei , Sun Liying , Yan Yan
Published: October 2019

Background: Nocardia is an opportunistic pathogen from environment, which is generally thought to infect immunosuppressed patients (ISPs), but recent studies showed it could also cause infections in immunocompetent patients (ICPs). Objective: To compare the clinical characteristics, patients’ outcome, Nocardia species identification and antibiotic susceptibility profiles of nocardiosis between ICPs and ISPs. Methods: The detailed clinical data were collected from all the non-repetitive nocardiosis patients during 2011 and 2018, from a tertiary general hospital in Beijing, China. Then each Nocardia isolate was identified to species level by DNA sequencing. The antibiotic susceptibility testing was performed by E-test method, and interpreted following CLSI M24 document. The clinical and microbiological characteristics between ICPs and ISPs were compared statistically. Results: A total of 23 non-repetitive nocardiosis patients with detailed clinical data were enrolled in this study. Among them, 9 were ICPs and 14 were ISPs. All the skin and soft tissue infections occurred in ICPs (33.3% versus 0%, P<0.05). Bronchiectasis occurred more frequently in ICPs (44.4% versus 21.4%), while chronic kidney diseases and co-infection with aspergillosis occurred more frequently in ISPs (35.7% versus 0%, 35.7% versus 0%, respectively), although they did not reach the statistical significance. There were no significant difference in other clinical characteristics, Nocardia species identification, and antibiotic susceptibility between ISPs and ICPs (P>0.05). Conclusion: Nocardiosis could occur in both ISPs and ICPs. Skin and soft tissue infection and bronchiectasis occurred more frequently in ICPs. Chronic kidney diseases and co-infection with aspergillosis occurred more frequently in ISPs. These characteristics should be noticed by physicians in diagnosis of nocardiosis.
Trabecular bone scores in young HIV-infected men: a matched case-control study
Authors: Kim Youn Jeong , Kang Kwi Young , Jun Yoonhee , Kim Sang Il , Ree Kim Yang
Published: October 2019

Background Screening for osteoporosis with dual-energy X-ray absorptiometry (DXA) is recommended for male HIV-infected patients only above the age of 50. Recently, trabecular bone score (TBS) has been introduced as a novel tool to assess bone microarchitecture using DXA of the lumbar spine. Few studies have reported TBS values in HIV-infected individuals younger than 50 years of age. This study compared TBS values in young males infected with HIV and matched controls, and investigated the associations between TBS and demographic parameters, clinical parameters, and bone mineral density (BMD) scores. Methods A cross-sectional study of BMD and TBS in HIV-infected men (n = 80) aged between 18 and 50 years and age- and sex-matched controls (n = 80) was conducted. Results The proportion of patients with low BMD (Z-score ≤−2) was significantly greater among HIV-infected patients than among matched controls (21.3% [17/80] vs. 8.8% [7/80], p = 0.027). Mean TBS values were significantly lower in HIV-infected patients than in controls (1.41 ± 0.07 vs. 1.45 ± 0.07, p = 0.008). In both groups, TBS values were positively correlated with BMD at the lumbar spine, femoral neck, and total hip (p < 0.001); however, TBS was not correlated with body mass index. In the HIV group, TBS was negatively correlated with the duration of tenofovir disoproxil fumarate(TDF) exposure (p = 0.04). Conclusion Young men infected with HIV had abnormal bone trabecular microarchitecture, as assessed by both TBS and BMD. TBS values were correlated with both BMD and the duration of TDF exposure.
Modulation of SIRT3 expression through CDK4/6 enhances the anti-cancer effect of sorafenib in hepatocellular carcinoma cells
Authors: Jo HanHee , Yoo Dayoung , Lee JongSook , Kim Seon yoo , Chung Jee-in , Ko Hae yong , Lee Misu , Yun Mijin
Published: October 2019

Background : Hepatocellular carcinoma (HCC) is the fifth leading cause of cancer-related deaths worldwide. The only drug currently approved for clinical use in the treatment of advanced HCC is sorafenib. However, many patients with HCC show reduced sensitivity to sorafenib during treatment. SIRT3, a member of the mammalian sirtuin family, is a tumor suppressor in certain tumor types. To date, a few studies have investigated the effects of SIRT3 on tumor prognosis and sorafenib sensitivity in patients with HCC. Thus, this study aimed to investigate the correlation between SIRT3 expression and glucose metabolism and proliferation in HCC and to discover effective compounds that increase endogenous SIRT3 modulation effect of sorafenib. Methods: To determine the correlation between SIRT3 and glucose related proteins, immunostaining was performed with liver cancer tissue using various antibodies. To investigate whether the expression of SIRT3 in HCC is related to the resistance to sorafenib, we treated sorafenib after the modulation of SIRT3 levels in HCC cell lines (overexpression in Huh7, knockdown in HepG2). We also employed PD0332991 to modulate the SIRT3 expression in HCC cell and conducted functional assays. Results: SIRT3 expression is downregulated in high glycolytic and proliferative HCC of human patients, xenograft model and HCC cell lines. We also demonstrated that SIRT3 expression was downregulated after sorafenib treatment, resulting in reduced drug sensitivity in HCC cell lines. To enhance the anti-tumor effect of sorafenib, we employed PD0332991 (CDK4/6-Rb inhibitor) based on the correlation between SIRT3 and phospho-Rb in HCC. In addition, combined treatment with sorafenib and PD0332991 showed a synergic anti-tumor effect in HCC cells. Conclusions: Taken together, our data suggest that the modulation of SIRT3 by CDK4/6 inhibition might be used for HCC therapy together with sorafenib, which, unfortunately, has limited efficacy and whose use is often associated with drug resistance.