bluuebunny/crossref_metadata_2025 huggingface dataset:
Environmental Effects on the Coefficient of Friction and Tack Adhesion of Formulated Waterborne Coatings
Authors: Hall J.L. , Bevas C.J. , Hinder S.J. , Kynaston E.L. , Lindsay C.I. , Gruber P. , Keddie J.L.
Published: January 2022

Waterborne coatings emit a low amount of harmful volatile organic compounds (VOCs) into the atmosphere compared to solvent-cast coatings. A typical waterborne formulation for agricultural applications consists of colloidal thermoplastic particles (latex) as the binder, a thickener to raise the viscosity, inorganic filler particles with a water-soluble dispersant, and a colloidal wax to modify surface properties. The formulations typically contain hygroscopic species that are potentially subject to softening by environmental moisture. The hardness, tack adhesion, and coefficient of friction of formulated coatings determines their suitability in applications. However, the relationship of these properties to the components in a coating formulation has not been adequately explored. Furthermore, the relationship between hygroscopic components and properties is an added complication. Here, we have characterized the hardness and tack adhesion of model formulated coatings using a single micro-indentation cycle with a conical indenter under controlled temperatures (above and below the glass transition temperature of a latex binder) and relative humidities. In parallel, we measured the coefficient of kinetic friction, μk, for the same coatings using a bespoke testing rig under controlled environmental conditions. Across a range of temperatures, RH and compositions, we find an inverse correlation between the coating hardness and μk. Any correlation of μk with the roughness of the coatings, which varies with the composition, is less clear. Formulations that contain wax additives have a higher μk at a low RH of 10%, in comparison to formulations without wax. For the wax formulations, μk decreases when the RH is raised, whereas in non-wax formulations, μk increases with increasing RH. Wax-containing coatings are hydrophilic (with a lower water contact angle), however the wax has a lower water permeability. A lubricating layer of water can explain the lower observed μk in these formulations. The addition of wax is also found to planarize the coating surface, which leads to higher tack adhesion in dry coatings in comparison to coatings without wax. Greater adhesive contact in these coatings can explain their higher friction. Our systematic research will aid the design of seed coating formulations to achieve their optimum properties under a wide range of environmental conditions.
Quantitative Analysis of Retinal Vasculature in Rhegmatogenous Retinal Detachment Based on Ultra-Widefield Fundus Imaging
Authors: Feng Bingkai , Su Wenxin , Chen Qingshan , Gan Run , Wang Mingxuan , Wang Jiantao , Zhang Jiayi , Yan Xiaohe
Published: January 2022

Purpose

To quantitatively analyze retinal vascular morphological features, such as vascular density, caliber, and tortuosity, in rhegmatogenous retinal detachment (RRD).

Methods

A total of 244 patients with RRD and 400 healthy controls (HC) were included. Retinal fundus images were collected using OPTOS PLC Daytona P200T. Retinal images were divided into RRD and non-RRD regions of interest (ROIs). All visible retinal fundus vessels were then extracted mainly based on edge detection within ROI to form the whole-vascular image. Retinal vasculature parameters, such as vascular density, caliber, and tortuosity, were calculated.

Results

For the absolute density, the mean rank (MR) value of normal controls was significantly higher than that in non-RRD (p < 0.001). A consistent tendency of significant vascular density was increased from non-RRD to RRD (p < 0.001). The average and median diameters of normal controls were both significantly larger than RRD (p < 0.001). The average and median diameters were also appeared significantly thinner in non-RRD. Unweighted and width-inversely-weighted vascular tortuosity in RRD and non-RRD comparison exhibited non-significant differences. All types of tortuosity calculated from HC were significantly larger (p < 0.001) in values compared to RRD. All types of tortuosity values of HC were significantly higher than non-RRD. Compared with non-RRD, RRD was significantly larger in area-weighted, length-weighted, and width-weighted vascular tortuosity.

Conclusions

This study showed that RRD affects both the quantity and morphology of retinal vasculature, such as RRD and non-RRD areas. Smaller average and medium vascular diameters and tortuosity values were found in RRD. However, the absolute vascular density, the average and median diameter, and tortuosity values were also reduced in non-RRD although the retina is still attached. This work indicates that RRD may affect the retinal vasculature beyond the detached retina.
A Simple Weaning Model Based on Interpretable Machine Learning Algorithm for Patients With Sepsis: A Research of MIMIC-IV and eICU Databases
Authors: Liu Wanjun , Tao Gan , Zhang Yijun , Xiao Wenyan , Zhang Jin , Liu Yu , Lu Zongqing , Hua Tianfeng , Yang Min
Published: January 2022

Background

Invasive mechanical ventilation plays an important role in the prognosis of patients with sepsis. However, there are, currently, no tools specifically designed to assess weaning from invasive mechanical ventilation in patients with sepsis. The aim of our study was to develop a practical model to predict weaning in patients with sepsis.

Methods

We extracted patient information from the Medical Information Mart for Intensive Care Database-IV (MIMIC-IV) and the eICU Collaborative Research Database (eICU-CRD). Kaplan–Meier curves were plotted to compare the 28-day mortality between patients who successfully weaned and those who failed to wean. Subsequently, MIMIC-IV was divided into a training set and an internal verification set, and the eICU-CRD was designated as the external verification set. We selected the best model to simplify the internal and external validation sets based on the performance of the model.

Results

A total of 5020 and 7081 sepsis patients with invasive mechanical ventilation in MIMIC-IV and eICU-CRD were included, respectively. After matching, weaning was independently associated with 28-day mortality and length of ICU stay (p < 0.001 and p = 0.002, respectively). After comparison, 35 clinical variables were extracted to build weaning models. XGBoost performed the best discrimination among the models in the internal and external validation sets (AUROC: 0.80 and 0.86, respectively). Finally, a simplified model was developed based on XGBoost, which included only four variables. The simplified model also had good predictive performance (AUROC:0.75 and 0.78 in internal and external validation sets, respectively) and was developed into a web-based tool for further review.

Conclusions

Weaning success is independently related to short-term mortality in patients with sepsis. The simplified model based on the XGBoost algorithm provides good predictive performance and great clinical applicablity for weaning, and a web-based tool was developed for better clinical application.
Kiss1 Inhibits the Proliferation of Nasopharyngeal Carcinoma Cells Via Activation of the LKB1/AMPK Pathway
Authors: Li Tingting , Tian Yong , Wang Yixuan , Cui Zhen , He Zelai , Wu Xiao , Zhang Yajun , Jiang Hao
Published: January 2022

Nasopharyngeal carcinoma (NPC) is a cancer that occurs in the nasopharynx. Infinite proliferation and distant metastasis are the main characteristics of NPC cells, and the main reason for the current failure of malignant tumor treatment. In this study, by integrating the immunohistochemical, cell transfection, western blot and real-time reverse transcriptase polymerase chain reaction (RT-PCR) analysis, we observed that the expression of KISS1 and its receptor gene (KISS1R) negatively related with the proliferation of NPC cells. Overexpression of the KISS1 genes in cells reduced cell proliferation, slow down the cell cycle, and increased apoptosis. Additionally, overexpression of these genes significantly increased Liver Kinase B1 (LKB1), phosphorylation of LKB1 and AMPK, indicated by Western blotting. Together, all of these results suggested for the first time that KISS1 and KISS1R suppress the proliferation of NPC cells by activating the LKB1/AMPK pathway, thus revealing a viable indicator for diagnosis of NPC in clinical practice.
Endoscope-Assisted Retrosigmoid Approach for Vestibular Schwannomas With Intracanalicular Extensions: Facial Nerve Outcomes
Authors: Bi Yunke , Ni Yunjia , Gao Dandan , Zhu Qingwei , Zhou Qiangyi , Tang Junjia , Liu Juan , Shi Fei , Li Hongchan , Yin Jian , Liu Yaohua , Lou Meiqing
Published: January 2022

Objective

To explore the role of neuroendoscope assistance during surgical resection of the intracanalicular portion of vestibular schwannomas via the retrosigmoid approach and the subsequent early facial nerve outcomes.

Methods

Patients of vestibular schwannoma with intracanalicular extensions undergoing retrosigmoid dissection at a single institution were retrospectively analyzed in this study. Several surgical techniques were applied to ensure maximal and safe removal of tumors. Tumors extending less than 10 mm into the internal acoustic canal (IAC) were classified as Grade A, while those extending over 10 mm into IAC were taken as Grade B. Neuroendoscope was applied at the end of microscopic phase to search for potential remnants for Grade B tumors. Absolute tumor extension was defined and measured. House and Brackmann (HB) scale was used to evaluate immediate CN VII outcomes.

Results

Of the 61 patients, there were 38 females and 23 males. A total of 18 (29.51%) cases were Koos Grade II, 12 (19.67%) cases Koos Grade III, and 31 (50.82%) cases Koos Grade IV. There were 38 cases (62.30%) of Grade A and 23 cases (37.70%) of Grade B. Gross total resection was achieved in 60 cases (98.36%). Four cases of intracanalicular remnants were detected and completely removed under endoscopic visualizations. There was a significantly higher proportion (17%, p = 0.02) of intracanalicular remnants in Grade B than Grade A. CN VII and VIII were anatomically preserved in all cases. A total of 55 cases (90.16%) retained good (HB Grades 1 and 2) facial nerve outcomes.

Conclusions

In Grade B vestibular schwannomas, after maximal microsurgical removal, endoscopic evaluation of the intracanalicular portion revealed residual tumors in 17% of the patients. Hence endoscopic evaluation of the potential intracanalicular remnants for tumor extending over 10 mm within IAC (Grade B) is recommended.
Overexpression of PD-L1 is an Independent Predictor for Recurrence in HCC Patients Who Receive Sorafenib Treatment After Surgical Resection
Authors: Tan Yifei , Xu Qing , Wu Zhenru , Zhang Wei , Li Bo , Zhang Bohan , Xu Xi , Zhang Bo , Yan Ke , Song Jiulin , Lv Tao , Yang Jian , Jiang Li , Shi Yujun , Yang Jiayin , Yan Lunan
Published: January 2022

Objective

The predicting values of programmed cell death protein 1 (PD-1) and programmed death-ligand 1(PD-L1) were unclear in Hepatocellular carcinoma (HCC) patients who receive sorafenib treatment after curative hepatic resection.

Methods

We retrospectively enrolled HCC patients who received adjuvant sorafenib treatment after curative resection (N = 154), and patients had resection alone (N = 312). Immunohistochemistry was used to assess expression of PD-1 on tumor infiltration immune cells and PD-L1 on HCC cells. Cox proportional hazard models were used to explore association between clinicopathological factors and risk of tumor recurrence.

Results

No significant difference was detected in RFS (p = 0.542), or OS (p = 0.542) between the resection and sorafenib group and resection alone group. In the 154 patients who received adjuvant sorafenib, expression of PD-1 or PD-L1 was not significantly associated with long-term outcomes. However, in the 122 patients at high risk of postoperative recurrence who had adjuvant sorafenib treatment, characterized by maxim tumor size ≥5 cm, or the presence of macro- or micro-vascular invasion, patients with PD-L1 overexpression (≥3.0) had significantly worse RFS (p = 0.021), and overexpression of PD-L1 (HR: 1.88, 95%CI: 1.18–2.99, p = 0.008) was identified as an independent risk factor associated with unfavorable RFS.

Conclusion

Overexpression of PD-L1 serves as an independent predictor of recurrence in HCC patients at high risk of relapse who received adjuvant sorafenib treatment after curative resection.
Vital Hepatic Lymphoma Residuum or Excessive Immune Response? Challenging Treatment Decisions in a Patient With Systemic Lupus Erythematosus and Liver-Dominant Diffuse Large B-Cell Lymphoma: Case Report
Authors: Kurch Lars , Georgi Thomas W. , Monecke Astrid , Seehofer Daniel , Borte Gudrun , Sabri Osama , Kluge Regine , Heyn Simone , Pierer Matthias , Platzbecker Uwe , Kayser Sabine
Published: January 2022

A 28-year-old female patient with active and difficult-to-treat systemic lupus erythematosus (SLE) was diagnosed with liver-dominant diffused large B-cell lymphoma. Repeated response 18F-FDG-PET studies showed persistently high, and, despite intensified immunochemotherapy, further increasing metabolic activity of one of the hepatic lymphoma residuals, whereas all other initial lymphoma manifestations had achieved complete metabolic remission. As biopsy of the 18F-FDG-PET-positive liver residual turned out to be inconclusive, complete resection was performed. Subsequent histopathological examination, however, revealed only necrotic tissue. Thus, no further lymphoma treatment was scheduled. The patient undergoes regular surveillance and is disease-free 13 months after resection. Similarly, treatment of SLE is no longer required due to lack of activity already after the first two cycles of lymphoma treatment. The case shows how closely SLE and diffused large B-cell lymphoma can be connected and stresses the importance of interdisciplinary treatment approaches. In the future, artificial intelligence may help to further classify 18F-FDG-PET-positive lymphoma residuals. This could lead to an increase of the positive predictive value of interim- and end-of-treatment 18F-FDG-PET. The patient’s point of view enables another instructive perspective on the course of treatment, which often remains hidden to treating physicians due to lack of time in clinical routine.
Efficacy and Safety of Oral Acetaminophen for Premature Infants With Patent Ductus Arteriosus: A Meta-Analysis
Authors: Zi-Yun Xie , Ruo-lin Zhang , Yue-wei Xia , Tao Bo
Published: January 2022

Objective: To systematically review the efficacy and safety of oral Acetaminophen for premature infants with patent ductus arteriosus (PDA).

Methods: Databases including Ovid, EMbase, Pubmed, The Cochrane Library, Cumulative Index to Nursing and Allied Health Literature (CINHAL), China National Knowledge Infrastructure (CNKI), Chinese Biomedical Database (CBM), WanFang Data, China Science and Technology Journal Database were searched to collect the randomized controlled trials (RCTs) about Acetaminophen for premature infants with PDA from inception to January 1, 2021. Quality assessment was performed through bias risk evaluation according to the Cochrane Handbook 5.1.0, and then the homogeneous studies were analyzed using Revman 5.4 software.

Results: A total of 16 RCTs were included, which were divided into for four subgroups: subgroup I (oral acetaminophen vs. oral ibuprofen, 13 RCTs), subgroup II (oral acetaminophen vs. intravenous indomethacin, 1 RCT), subgroup III (oral acetaminophen vs intravenous ibuprofen, 1 RCT), and subgroup IV (oral acetaminophen vs intravenous placebo, 1 RCT). In subgroup I, There was no significant difference in the ductal closure rate after the first course of drug administration [typical relative risk (RR) 0.97, 95% confidence interval (CI) 0.90 to 1.05], the accumulated ductal closure rate after two course of treatment (RR 0.96, 95% CI 0.91–1.02), and mortality (RR 1.06, 95% CI 0.75–1.49) between treatment with oral acetaminophen versus oral ibuprofen (p > 0.05); compared with oral ibuprofen, oral acetaminophen was associated with a significant reduction in the incidence of gastrointestinal bleeding/stool occult blood positive (RR 0.51, 95% CI 0.32 to 0.82)and oliguria (RR 0.62, 95% CI 0.42–0.91) (p < 0.05).

Conclusion: The meta analysis approves the facts that there is no significant difference in the efficacity in premature infants with PDA between oral acetaminophen and buprofen or indometacin, but compared to ibuprofen, oral acetaminophen may decrease the incidence of oliguria and gastrointestinal bleeding. More reliable conclusions should be made through large-size, multi-center, well-designed RCTs.
Cilostazol Ameliorates Peripheral Neuropathic Pain in Streptozotocin-Induced Type I Diabetic Rats
Authors: Cheng Kuang-I. , Wang Hung-Chen , Tseng Kuang-Yi , Wang Yi-Hsuan , Chang Chung-Yu , Chen Yi-Jing , Lai Chung-Sheng , Chen Dar-Ren , Chang Lin-Li
Published: January 2022

Background: Cilostazol is an antiplatelet agent with vasodilating, endothelial function restoration, and anti-inflammatory effects. This study aims to investigate the efficacy of oral cilostazol for preventing the development of diabetic peripheral neuropathy (DPN).

Materials and Methods: Ninety adult male Sprague-Dawley rats were divided into five groups: 1) naïve (control); 2) diabetic (DM); 3) DM receiving 10 mg/kg cilostazol (cilo-10); 4) DM receiving 30 mg/kg cilostazol (cilo-30); and 5) DM receiving 100 mg/kg cilostazol (cilo-100). Hindpaw responses to thermal and mechanical stimuli were measured. Activation of microglia and astrocytes in the spinal dorsal horn (SDH) and expression of NaVs in the dorsal root ganglia (DRG) were examined with Western blots and immunofluorescence.

Results: DM rats displayed decreased withdrawal thresholds to mechanical stimuli (mechanical allodynia) and blunted responses to thermal stimuli. In addition, the expression of microglia increased, but astrocytes were reduced in the SDH. Upregulation of Nav −1.1, 1.2, −1.3, −1.6, and −1.7 and downregulation of Nav-1.8 were observed in the DRG. The DM rats receiving cilostazol all returned DM-induced decrease in withdrawal threshold to mechanical stimuli and attenuated neuropathic pain. Additionally, all cilostazol treatments suppressed the level of activated microglial cells and ameliorated the DM-induced decline in astrocyte expression levels in the SDH. However, only the rats treated with cilo-100 demonstrated significant improvements to the aberrant NaV expression in the DRG.

Conclusion: Oral cilostazol can blunt the responses of mechanical allodynia and has the potential to treat diabetic neuropathy by attenuating NaV and glial cell dysregulation.
PBK Enhances Cellular Proliferation With Histone H3 Phosphorylation and Suppresses Migration and Invasion With CDH1 Stabilization in Colorectal Cancer
Authors: Koshino Akira , Nagano Aya , Ota Akinobu , Hyodo Toshinori , Ueki Akane , Komura Masayuki , Sugimura-Nagata Akane , Ebi Masahide , Ogasawara Naotaka , Kasai Kenji , Hosokawa Yoshitaka , Kasugai Kunio , Takahashi Satoru , Inaguma Shingo
Published: January 2022

Colorectal cancer (CRC) is one of the most frequent gastrointestinal malignancies with high morbidity and mortality rates. Several biological markers for the prognostication of patient outcome of CRCs are available. Recently, our group identified two favorable factors for the survival of CRC patients: PDZ-binding kinase (PBK) and phospho-histone H3 (PHH3). Both showed a significant inverse association to pT stage. The aim of this study was to uncover the mechanism through which these cellular proliferation–associated protein expressions lead to favorable clinical outcome in CRC patients. We first confirmed co-expression of PBK and PHH3 in CRC cells. Further investigation showed that aberrantly expressed PBK up-regulated the cellular proliferation of CRC cells with accumulation of PHH3. The PBK inhibitor OTS514 suppressed cellular proliferation of CRC cells through down-regulation of PHH3 and induction of apoptosis. In vitro studies revealed that PBK suppressed the migration and invasion of CRC cells with suppression of Wnt/β-catenin signaling and CDH1 stabilization. Exogeneous PBK up-regulated the phosphorylated CDH1 at S840, S846, and S847 residues in cultured cells. Recombinant PBK directly phosphorylated HH3; however, it failed to phosphorylate CDH1 directly in vitro. The present study demonstrated the association of two markers PBK and PHH3 in CRC. We further identified one of the potential mechanisms by which higher expression of these cellular proliferation–associated proteins leads to the better survival of CRC patients, which likely involves PBK-mediated suppression of the migration and invasion of CRC cells. Our findings suggest that PBK-targeting therapeutics may be useful for the treatment of CRC patients with PBK-expressing tumors.
Autophagy Induced by Micheliolide Alleviates Acute Irradiation-Induced Intestinal Injury via Inhibition of the NLRP3 Inflammasome
Authors: Wu Dong-ming , Li Jing , Shen Rong , Li Jin , Yu Ye , Li Li , Deng Shi-hua , Liu Teng , Zhang Ting , Xu Ying , Wang De-gui
Published: January 2022

Radiation-induced enteropathy (RIE) is one of the most common and fatal complications of abdominal radiotherapy, with no effective interventions available. Pyroptosis, a form of proinflammatory regulated cell death, was recently found to play a vital role in radiation-induced inflammation and may represent a novel therapeutic target for RIE. To investigate this, we found that micheliolide (MCL) exerted anti-radiation effects in vitro. Therefore, we investigated both the therapeutic effects of MCL in RIE and the possible mechanisms by which it may be therapeutic. We developed a mouse model of RIE by exposing C57BL/6J mice to abdominal irradiation. MCL treatment significantly ameliorated radiation-induced intestinal tissue damage, inflammatory cell infiltration, and proinflammatory cytokine release. In agreement with these observations, the beneficial effects of MCL treatment in RIE were abolished in Becn1+/− mice. Furthermore, super-resolution microscopy revealed a close association between NLR pyrin domain three and lysosome-associated membrane protein/light chain 3-positive vesicles following MCL treatment, suggesting that MCL facilitates phagocytosis of the NLR pyrin domain three inflammasome. In summary, MCL-mediated induction of autophagy can ameliorate RIE by NLR pyrin domain three inflammasome degradation and identify MCL as a novel therapy for RIE.
Pterocarpus santalinus Selectively Inhibits a Subset of Pro-Inflammatory Genes in Interleukin-1 Stimulated Endothelial Cells
Authors: Natalia Priscilla , Zwirchmayr Julia , Rudžionytė Ieva , Pulsinger Alexandra , Breuss Johannes M. , Uhrin Pavel , Rollinger Judith M. , de Martin Rainer
Published: January 2022

Based on the traditional use and scientific reports on the anti-inflammatory potential of red sandalwood, i.e., the heartwood of Pterocarpus santalinus L., we investigated its activity in a model of IL-1 stimulated endothelial cells. Endothelial cells were stimulated with IL-1 with or without prior incubation with a defined sandalwoodextract (PS), and analyzed for the expression of selected pro-inflammatory genes. The activity of NF-κB, a transcription factor of central importance for inflammatory gene expression was assessed by reporter gene analysis, Western blotting of IκBα, and nuclear translocation studies. In addition, microarray studies were performed followed by verification of selected genes by qPCR and supplemented by bioinformatics analysis. Our results show that PS is able to suppress the induction of E-selectin and VCAM-1, molecules that mediate key steps in the adhesion of leukocytes to the endothelium. It also suppressed the activity of an NF-κB reporter, IκBα phosphorylation and degradation, and the nuclear translocation of NF-κB RelA. In contrast, it stimulated JNK phosphorylation indicating the activation of the JNK signaling pathway. Gene expression profiling revealed that PS inhibits only a specific subset of IL-1 induced genes, while others remain unaffected. Most strongly suppressed genes were the signal transducer TRAF1 and the chemokine CX3CL1, whereas IL-8 was an example of a non-affected gene. Notably, PS also stimulated the expression of certain genes, including ones with negative regulatory function, e.g., members of the NR4A family, the mRNA destabilizing protein TTP as well as the transcription factors ATF3 and BHLHB40. These results provide mechanistic insight into the anti-inflammatory activity of PS, and suggest that it acts through the interplay of negative and positive regulators to achieve a differential inhibition of inflammatory gene expression.
Anti-Inflammatory and Pro-Autophagy Effects of the Cannabinoid Receptor CB2R: Possibility of Modulation in Type 1 Diabetes
Authors: Liu Qing-Rong , Aseer Kanikkai Raja , Yao Qin , Zhong Xiaoming , Ghosh Paritosh , O’Connell Jennifer F. , Egan Josephine M.
Published: January 2022

Type 1 diabetes mellitus (T1DM) is an autoimmune disease resulting from loss of insulin-secreting β-cells in islets of Langerhans. The loss of β-cells is initiated when self-tolerance to β-cell-derived contents breaks down, which leads to T cell-mediated β-cell damage and, ultimately, β-cell apoptosis. Many investigations have demonstrated the positive effects of antagonizing cannabinoid receptor 1 (CB1R) in metabolic diseases such as fatty liver disease, obesity, and diabetes mellitus, but the role of cannabinoid receptor 2 (CB2R) in such diseases is relatively unknown. Activation of CB2R is known for its immunosuppressive roles in multiple sclerosis, rheumatoid arthritis, Crohn’s, celiac, and lupus diseases, and since autoimmune diseases can share common environmental and genetic factors, we propose CB2R specific agonists may also serve as disease modifiers in diabetes mellitus. The CNR2 gene, which encodes CB2R protein, is the result of a gene duplication of CNR1, which encodes CB1R protein. This ortholog evolved rapidly after transitioning from invertebrates to vertebrate hundreds of million years ago. Human specific CNR2 isoforms are induced by inflammation in pancreatic islets, and a CNR2 nonsynonymous SNP (Q63R) is associated with autoimmune diseases. We collected evidence from the literature and from our own studies demonstrating that CB2R is involved in regulating the inflammasome and especially release of the cytokine interleukin 1B (IL-1β). Furthermore, CB2R activation controls intracellular autophagy and may regulate secretion of extracellular vesicles from adipocytes that participate in recycling of lipid droplets, dysregulation of which induces chronic inflammation and obesity. CB2R activation may play a similar role in islets of Langerhans. Here, we will discuss future strategies to unravel what roles, if any, CB2R modifiers potentially play in T1DM.
The Negative Dependence of Evacuation Time on Group Size Under a Binding Mechanism
Authors: Wang Tianyi , Chen Yu
Published: January 2022

This paper initiates the analysis of the relation between evacuation time and group size by applying an extended floor-field cellular automaton model. Multi-speed-agents, a group structure containing leaders and followers, and a local-density-dependent dynamic field are implemented all together in the model. Most importantly, a complete binding mechanism which includes leaders waiting for followers is brought up for the first time. A counterintuitive negative relation between evacuation time and group size is discovered in one-group-type simulations, which corresponds to a previous experimental finding. An entropy-like quantity, namely the mixing index, is constructed to analyze the cause of that relation. It is found that under the binding mechanism, the higher degree of group mixing, the longer the evacuation time will be. Moreover, through a constant scale transformation, it is shown that the mixing index can be a key indicator that contains useful information about the evacuation system.
Engineering a Plant-Derived Astaxanthin Synthetic Pathway Into Nicotiana benthamiana
Authors: Allen Quinton M. , Febres Vicente J. , Rathinasabapathi Bala , Chaparro José X.
Published: January 2022

Carotenoids have been shown to be essential for human nutrition. Consumption of carotenoid-rich fruits and vegetables can reduce the risk of many diseases. The ketocarotenoid astaxanthin has become a commercially valuable compound due to its powerful antioxidant properties compared to other carotenoids. It is naturally produced in certain algae, bacteria, and the flowers of some species of the genus Adonis, although it is produced in such small quantities in these organisms that it is costly to extract. Chemical synthesis of this compound has also shown limited success with a high proportion of esterified forms of astaxanthin being produced, which decreases antioxidant properties by the conversion of hydroxyl groups to esters. Previously, transgenic astaxanthin-producing plants have been created using a β-carotene ketolase enzyme of either bacterial or algal origin. However, a novel astaxanthin pathway exists in the flowering plants of the genus Adonis which has not been utilized in the same manner. The pathway involves two unique enzymes, β-ring-4-dehydrogenase and 4-hydroxy-β-ring-4-dehydrogenase, which add the necessary hydroxyl and ketone groups to the rings of β-carotene. In the present study, Nicotiana benthamiana plants were transformed with chimeric constructs coding for these two enzymes. The regenerated, transgenic plants accumulate astaxanthin and their growth (height and weight) was unaffected, when compared to non-transformed N. benthamiana and to plants transformed with the bacterial β-carotene ketolase. The accumulation of astaxanthin also improved seedling survivability under harsh UV light, mitigated reactive oxygen accumulation, and provided a phenotype (color) that allowed the efficient identification and recovery of transgenic plants with and without selection.
A Configurational Analysis of Small and Medium-Sized Enterprises’ Radical Innovations: The Perspective of Dynamic Capabilities
Authors: Tang Shuangshuang , Liao Shudi , Wang Lumeng , Chen Weijing , Guo Zhiwen
Published: January 2022

Adopting a configurational perspective, this study explored the pathways for small and medium-sized enterprises (SMEs) to achieve high levels of radical innovation. On the basis of dynamic capabilities theory, six causal conditions for radical innovation were identified at both external and internal levels—that is, environmental turbulence (i.e., technological and market turbulence) and absorptive capacity (i.e., knowledge base, explorative, transformative, and exploitative learning processes). The results of a fuzzy-set qualitative comparative analysis (fsQCA) of 82 Chinese SMEs identified four solutions for high radical innovation. The six causal conditions interacted interdependently and different combinations of these conditions were equally effective pathways for SMEs to achieve radical innovation. Hence, SMEs could generate radical innovation through flexibly allocating resources and capabilities based on the environmental circumstances. By using the fsQCA method, this study contributes to the related literature with an investigation of the complex causal relationship between environmental turbulence, absorptive capacity, and SMEs’ radical innovation. The results resolve some prior contradictory findings and provide new insights for future research. Other theoretical contributions, practical implications, and directions for future research are also discussed.
A Hybrid Feature Selection and Ensemble Approach to Identify Depressed Users in Online Social Media
Authors: Liu Jingfang , Shi Mengshi
Published: January 2022

Depression has become one of the most common mental illnesses, and the widespread use of social media provides new ideas for detecting various mental illnesses. The purpose of this study is to use machine learning technology to detect users of depressive patients based on user-shared content and posting behaviors in social media. At present, the existing research mostly uses a single detection method, and the unbalanced class distribution often leads to a low recognition rate. In addition, a large number of irrelevant or redundant features in high-dimensional data sets interfere with the accuracy of recognition. To solve this problem, this paper proposes a hybrid feature selection and stacking ensemble strategy for depression user detection. First, recursive elimination method and extremely randomized trees method are used to calculate feature importance and mutual information value, calculate feature weight vector, and select the optimal feature subset according to the feature weight. Second, naive bayes, k-nearest neighbor, regularized logistic regression and support vector machine are used as base learners, and a simple logistic regression algorithm is used as a combination strategy to build a stacking model. Experimental results show that compared with other machine learning algorithms, the proposed hybrid method, which integrates feature selection and ensemble, has a higher accuracy of 90.27% in identifying online patients. We believe this study will help develop new methods to identify depressed people in social networks, providing guidance for future research.
Job Demands and Resources, Positive and Negative Affect, and Psychological Distress of Social Workers in China
Authors: Huang Chienchung , Xie Xiaoxia , Cheung Shannon P. , Zhou Yuqing
Published: January 2022

Globally, human service professionals, like social workers, experience significant job demands (JD) which can lead to outcomes like psychological distress, burnout, and high turnover rates. This is especially true in China, where the social work profession has grown substantially in recent decades. Because social workers play a crucial role in supporting vulnerable communities, there is a need to understand how their work conditions affect outcomes like psychological distress. This study applies the job demands and resources (JD-R) model to study this relation, along with the mediational effects of positive affect (PA) and negative affect (NA), in social workers from Chengdu, China (n = 897). The results of structural equation modeling indicate that JD-R differentially affect psychological distress. PA and NA partially mediate these relations. Job resources (JR) reduced psychological distress by reducing NA and increasing PA. JD did not have any effect on PA but significantly increased NA, which was associated with higher psychological distress. The magnitudes of each estimate suggest that JR has a greater effect on PA and NA, relative to the effects of JD on PA and NA. Interventions that seek to promote PA and reduce NA may be able to work with existing JR to buffer against the effects of JD in social workers.
Son or Daughter Care in Relation to Self-Reported Health Outcomes for Older Adults in China
Authors: Zhang Yanan , Harper Sarah
Published: January 2022

Objectives

Parental care in China is traditionally provided by sons. In recent decades—partly due to the One-Child Policy but also economic development and urbanization—significant changes have occurred with more and more parents receiving care from daughters. We investigate the disparities in outcomes of eldercare provided by son(s) and daughter(s).

Methods

Our study compares the self-reported health (SRH) status of parents who receive eldercare from daughters and sons in China, analyzing the harmonized 2013, 2015, and 2018 waves of CHARLS with random-effects logistic estimates.

Results

Our results show that the SRH status of parents who receive care from their sons is greater than those cared for by their daughters. This disparity is greater in rural areas, for mothers, and poorer families.

Discussion

The One-Child Policy was more effective in urban areas, reducing both the availability of sons and cultural son preference. Higher levels of education received by girls in urban settings increases their employability and thus their ability to materially care for their parents. However, traditional norms and gender differences in social economic statuses still persist in rural areas, leading to higher SRH status of those cared for by sons, especially amongst those who are heavily dependent on their children: mothers or parents with less wealth.
Cardiac Patch Transplantation Instruments for Robotic Minimally Invasive Cardiac Surgery: Initial Proof-of-concept Designs and Surgery in a Porcine Cadaver
Authors: Roche Christopher D. , Iyer Gautam R. , Nguyen Minh H. , Mabroora Sohaima , Dome Anthony , Sakr Kareem , Pawar Rohan , Lee Vincent , Wilson Christopher C. , Gentile Carmine
Published: January 2022

Background: Damaged cardiac tissues could potentially be regenerated by transplanting bioengineered cardiac patches to the heart surface. To be fully paradigm-shifting, such patches may need to be transplanted using minimally invasive robotic cardiac surgery (not only traditional open surgery). Here, we present novel robotic designs, initial prototyping and a new surgical operation for instruments to transplant patches via robotic minimally invasive heart surgery.

Methods: Robotic surgical instruments and automated control systems were designed, tested with simulation software and prototyped. Surgical proof-of-concept testing was performed on a pig cadaver.

Results: Three robotic instrument designs were developed. The first (called “Claw” for the claw-like patch holder at the tip) operates on a rack and pinion mechanism. The second design (“Shell-Beak”) uses adjustable folding plates and rods with a bevel gear mechanism. The third (“HeartStamp”) utilizes a stamp platform protruding through an adjustable ring. For the HeartStamp, rods run through a cylindrical structure designed to fit a uniportal Video-Assisted Thorascopic Surgery (VATS) surgical port. Designed to work with or without a sterile sheath, the patch is pushed out by the stamp platform as it protrudes. Two instrument robotic control systems were designed, simulated in silico and one of these underwent early ‘sizing and learning’ prototyping as a proof-of-concept. To reflect real surgical conditions, surgery was run “live” and reported exactly (as-it-happened). We successfully picked up, transferred and released a patch onto the heart using the HeartStamp in a pig cadaver model.

Conclusion: These world-first designs, early prototypes and a novel surgical operation pave the way for robotic instruments for automated keyhole patch transplantation to the heart. Our novel approach is presented for others to build upon free from restrictions or cost—potentially a significant moment in myocardial regeneration surgery which may open a therapeutic avenue for patients unfit for traditional open surgery.
Do Certification Schemes Enhance Coffee Yields and Household Income? Lessons Learned Across Continents
Authors: Jena Pradyot Ranjan , Grote Ulrike
Published: January 2022

While the market for sustainably certified products grows, the debate on whether smallholder farmers benefit from this certification movement is far from over. We present empirical findings across three continents. Identical household surveys were conducted among 738 smallholder coffee farmers organized in primary cooperatives in Ethiopia, India and Nicaragua. The comparative analysis which is based on the propensity score matching approach shows that the impacts of Fairtrade certification on coffee yields and income vary across countries. In Ethiopia, the coffee farmers from Fairtrade certified cooperatives fare worse than their non-certified counterparts both in coffee yield and income. In the Indian case study, the Fairtrade cooperative members have yield and price advantages over the non-certified farmers. This has in turn led to higher net revenue from coffee for certified farmers. In Nicaragua, coffee farmers from Fairtrade and double (Fairtrade-Organic) certified cooperatives also benefit in terms of net revenue but there is no statistically significant effect on yield and household income. A comparison of the Fairtrade minimum floor price and the weight-equivalent Fairtrade cooperative price in the three countries shows that Nicaraguan Fairtrade certified farmers have obtained a higher average price than the Fairtrade mandated minimum price, whereas in Ethiopia the certified farmers received a much lower price. In India, the certified average price was closer to the minimum floor price. We conclude that coffee cooperatives and the motivation and capability of their staff play a central role in training their member farmers about each aspect of coffee growing and certification.
Metabolite Identification and Pharmacokinetic Behavior of Diaveridine in the Plasma of Pigs and Chickens Based on Radioactive Tracing Coupled With LC/MS-IT-TOF Assay
Authors: Wang Liye , Wen Lihua , Pan Yuanhu , Wang Zhenzhen , Zhou Kaixiang , Mi Kun , Liu Zhenli , Qu Wei , Huang Lingli
Published: January 2022

Diaveridine (DVD) is widely used for the prevention and treatment of coccidiosis and leucocytozoonosis infections in food-producing animals. To gain a better understanding of DVD metabolism and pharmacokinetics in healthy Landrace/Doric Cross castrated male pigs and both female and male Cobb 500 broiler chickens, a method involving radioactive tracing coupled with LC/MS-IT-TOF was developed for the identification and quantitation of DVD and its metabolites in pig and chicken plasma, and then was applied to investigate DVD pharmacokinetics. A simple MCX solid phase extraction procedure was adopted for sample preparation. After a single oral administration of 3H-DVD (10 mg/kg BW), three radioactive compounds (D0: DVD; D1: 3′-desmethyl-DVD; and D2: monoglucuronide of 3′-desmethyl-DVD) were identified in pig plasma, while only two radioactive compounds (D0 and D2) were identified in chicken plasma. In both species, the Cmax values for all detected compounds were reached at 2 h after dosing. The Cmax order was D2 (1.38 μg/ml) > D0 (0.49 μg/ml) > D1 (0.24 μg/ml) in pigs and D0 (1.55 μg/ml) > D2 (0.27 μg/ml) in chickens. The longer t1/2 (elimination half-life) of D0 contributed to the slow elimination of DVD-related compounds. The t1/2β of D0 in pigs (66.41 h) was significantly longer than that in chickens (48.30 h), but the t1/2 of total DVD-related metabolites in pigs (42.86 h) was lower than that in chickens (56.11 h). These findings suggested that the metabolism and pharmacokinetics of DVD in pigs and chickens were significantly different, and that this would affect its effectiveness, toxicology, and food safety in these animals.
GAE and OBE Enhanced Interference Mitigation Techniques in LDACS
Authors: Keshkar Miziya , Muthalagu Raja , Rajak Abdul , Mathew Libin K.
Published: January 2022

Interference mitigation in L-band digital aeronautic communication systems from legacy users is vital due to stringent safety requirements and steady-state increase in air traffic. This paper proposes an L-band digital aeronautic communication systems receiver prototype that employs nonlinear operations to reduce the interference from the prime interference contributor distance measuring equipment. The knowledge of genie-aided estimator and optimum Bayesian estimator is utilized to propose improved and low complexity nonlinear devices, such as a genie-aided estimator enhanced pulse peak attenuator, genie-aided estimator enhanced pulse peak limiter, joint genie-aided estimator enhanced pulse peak attenuator, joint genie-aided estimator enhanced pulse peak limiter, optimum Bayesian estimator enhanced pulse peak attenuator, optimum Bayesian estimator enhanced pulse peak limiter, joint optimum Bayesian estimator enhanced pulse peak attenuator and joint optimum Bayesian estimator enhanced pulse peak limiter. The performance of the proposed methods is compared with the classical pulse blanking in terms of the received bit error rate for different signal-to-noise ratios. The proposed genie-aided estimator enhanced methods exhibited SNR saving in the range of 2 to 2.5 dB at a bit error rate of 10−1. At the same BER, the proposed optimum Bayesian estimator enhanced methods achieved SNR saving in the range of 2.5 to 3 dB.
The Classification of Farming Progress in Rice–Wheat Rotation Fields Based on UAV RGB Images and the Regional Mean Model
Authors: Song Xiaoxin , Wu Fei , Lu Xiaotong , Yang Tianle , Ju Chengxin , Sun Chengming , Liu Tao
Published: January 2022

Extraction of farming progress information in rice–wheat rotation regions is an important topic in smart field research. In this study, a new method for the classification of farming progress types using unmanned aerial vehicle (UAV) RGB images and the proposed regional mean (RM) model is presented. First, RGB information was extracted from the images to create and select the optimal color indices. After index classification, we compared the brightness reflection of the corresponding grayscale map, the classification interval, and the standard deviation of each farming progress type. These comparisons showed that the optimal classification color indices were the normalized red–blue difference index (NRBDI), the normalized green–blue difference index (NGBDI), and the modified red–blue difference index (MRBDI). Second, the RM model was built according to the whole-field farming progress classification requirements to achieve the final classification. We verified the model accuracy, and the Kappa coefficients obtained by combining the NRBDI, NGBDI, and MRBDI with the RM model were 0.86, 0.82, and 0.88, respectively. The proposed method was then applied to predict UAV RGB images of unharvested wheat, harvested wheat, and tilled and irrigated fields. The results were compared with those obtained with traditional machine learning methods, that is, the support vector machine, maximum likelihood classification, and random forest methods. The NRBDI, NGBDI, and MRBDI were combined with the RM model to monitor farming progress of ground truth ROIs, and the Kappa coefficients obtained were 0.9134, 0.8738, and 0.9179, respectively, while traditional machine learning methods all produced a Kappa coefficient less than 0.7. The results indicate a significantly higher accuracy of the proposed method than those of the traditional machine learning classification methods for the identification of farming progress type. The proposed work provides an important reference for the application of UAV to the field classification of progress types.
Economic Advantages of Individual Animal Identification in Fattening Pigs
Authors: Thölke Henrich , Wolf Petra
Published: January 2022

Pig farmers are exposed to strong international competition and confronted with low revenues, while production requirements are increasing. Individual electronic monitoring allows for closer observation of the fattening process, which can be useful for identifying weaknesses in the production process and carrying out more targeted cost management. Consequently, this study aimed to demonstrate a reduction in unit production costs through individual animal identification (IAI) using ultra-high-frequency radio frequency identification (UHF RFID) transponder ear tags. For data collection, day-old suckling piglets were individually marked. During fattening, these piglets (n = 224) were kept in 16 groups (n = 14 in each). Individual body weights and the feed consumption for each pen were recorded until slaughtering. These data were used to perform three hypothetical selection scenarios at nine different times, retrospectively, by selecting individual animals that deviated downwards from the mean value of live weight by 10, 15, or 20%. For each group, two different calculations were performed to highlight potential savings. High potential savings of direct costs (up to EUR 4.56) for female animals and castrates (EUR 5.44) were shown. Nevertheless, costs of individual data collection were EUR 4.06 per pig, and we found that IAI offers advantages for conventional fatteners for future fattening periods to work in an economically sustainable manner.