bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of loci associated with water use efficiency and symbiotic nitrogen fixation in soybean
Authors: Arifuzzaman Muhammad , Mamidi Sujan , Sanz-Saez Alvaro , Zakeri Hossein , Scaboo Andrew , Fritschi Felix B.
Published: November 2023

Soybean (Glycine max) production is greatly affected by persistent and/or intermittent droughts in rainfed soybean-growing regions worldwide. Symbiotic N2 fixation (SNF) in soybean can also be significantly hampered even under moderate drought stress. The objective of this study was to identify genomic regions associated with shoot carbon isotope ratio (δ13C) as a surrogate measure for water use efficiency (WUE), nitrogen isotope ratio (δ15N) to assess relative SNF, N concentration ([N]), and carbon/nitrogen ratio (C/N). Genome-wide association mapping was performed with 105 genotypes and approximately 4 million single-nucleotide polymorphism markers derived from whole-genome resequencing information. A total of 11, 21, 22, and 22 genomic loci associated with δ13C, δ15N, [N], and C/N, respectively, were identified in two environments. Nine of these 76 loci were stable across environments, as they were detected in both environments. In addition to the 62 novel loci identified, 14 loci aligned with previously reported quantitative trait loci for different C and N traits related to drought, WUE, and N2 fixation in soybean. A total of 58 Glyma gene models encoding for different genes related to the four traits were identified in the vicinity of the genomic loci.
Research progress on the roles of actin-depolymerizing factor in plant stress responses
Authors: Sun Yongwang , Shi Mengmeng , Wang Deying , Gong Yujie , Sha Qi , Lv Peng , Yang Jing , Chu Pengfei , Guo Shangjing
Published: November 2023

Actin-depolymerizing factors (ADFs) are highly conserved small-molecule actin-binding proteins found throughout eukaryotic cells. In land plants, ADFs form a small gene family that displays functional redundancy despite variations among its individual members. ADF can bind to actin monomers or polymerized microfilaments and regulate dynamic changes in the cytoskeletal framework through specialized biochemical activities, such as severing, depolymerizing, and bundling. The involvement of ADFs in modulating the microfilaments’ dynamic changes has significant implications for various physiological processes, including plant growth, development, and stress response. The current body of research has greatly advanced our comprehension of the involvement of ADFs in the regulation of plant responses to both biotic and abiotic stresses, particularly with respect to the molecular regulatory mechanisms that govern ADF activity during the transmission of stress signals. Stress has the capacity to directly modify the transcription levels of ADF genes, as well as indirectly regulate their expression through transcription factors such as MYB, C-repeat binding factors, ABF, and 14-3-3 proteins. Furthermore, apart from their role in regulating actin dynamics, ADFs possess the ability to modulate the stress response by influencing downstream genes associated with pathogen resistance and abiotic stress response. This paper provides a comprehensive overview of the current advancements in plant ADF gene research and suggests that the identification of plant ADF family genes across a broader spectrum, thorough analysis of ADF gene regulation in stress resistance of plants, and manipulation of ADF genes through genome-editing techniques to enhance plant stress resistance are crucial avenues for future investigation in this field.
Understanding the impact of the COVID-19 pandemic on U.S. older adults: self-reported pandemic-related concerns and consequences in a cross-sectional survey study
Authors: Sams Nichole , Darnell Doyanne , Fisher Dylan , Allred Ryan , Huyhn Kathy , Mosser Brittany A. , Areán Patricia A.
Published: November 2023

Background and objectives

The purpose of this study was to explore COVID-19 pandemic-related concerns among a racially and ethnically representative sample of older adults in the U.S.

Research design and methods

Participants were 501 English-speaking adults 60 years and older recruited online nationally across the U.S. from Amazon Mechanical Turk (mTurk) and Prolific Research Platforms during June of 2020. Data comes from a larger cross-sectional survey. We content analyzed open-ended responses about pandemic-related concerns and observed responses to a checklist of items created by the research team to assess for specific physical, social, and financial consequences experienced due to the pandemic.

Results

A majority of the sample (92%) reported at least one pandemic-related concern, with the highest percentage expressing concerns coded as Concern for Others (28%), Physical Health (27%), Socializing (24%), Finance (15%) and Socio-Political-Economic (14%). Participants reported high concern severity (M = 4.03, SD = 1.04) about their concerns mentioned in response to the open-ended concerns question. When prompted with a checklist of items, participants frequently endorsed disruption in social activities as a consequence of the pandemic (83%), disruptions that could impact physical health (45%), and concern over finances as a consequence of the pandemic (41%).

Discussion and implications

Older adults most frequently mentioned concerns about the well-being and behavior of others, one’s own physical health, and the impacts of the pandemic and social distancing policies on social activities. Findings align with the Socioemotional Selectivity Theory and point to the importance of supporting older adults to maintain meaningful social engagement under conditions of a pandemic and social distancing policies.
Learning with self-generated drawings and the impact of learners’ emotional states
Authors: Navratil Sabrina D. , Kühl Tim
Published: November 2023

Introduction

The present study examined the influence of emotional states when learning with self-generated drawings. It was assumed that learners in a positive emotional state would profit from learning with self-generated drawings, while learners in a negative emotional state would not profit from this strategy to the same extent but would rather benefit through reading.

Methods

University students (N  =  123) were randomly assigned to one of four conditions resulting from a 2  ×  2 design with self-generated drawings (yes vs. no) and emotional state (positive vs. negative) as independent variables.

Results

Results showed that learning with self-generated drawings was more beneficial for a following transfer test than learning without drawings – irrespective of a learner’s emotional state. The quality of self-generated drawings predicted the learning outcomes of the retention and pictorial test, but not for transfer.

Discussion

Missing effects of emotional states and the missing interaction with self-generated drawings will be discussed.
Visual-motor involvement in autism spectrum disorder: could the stereopsis deficit affect motor coordination?
Authors: Longo Rosa , Allegrini Francesca , Gusson Elena , Morbio Roberta , Di Gennaro Gianfranco , Gozzi Luigi Alberto , Marchini Giorgio , Zoccante Leonardo
Published: November 2023

Purpose

Given the known difficulty in sensory processing and in motor skills in patients with Autism Spectrum Disorder (ASD), and since visual impairment could interfere with children’s behaviour, early detection and management of visual-motor difficulties are crucial. This exploratory study aims to evaluate the visual-motor status in a cohort of children affected by ASD.

Methods

The records of patients affected by ASD and admitted between 2018 and 2022 to the Pediatric University Hospital of Verona were reviewed. Best Corrected Visual Acuity, cycloplegic refraction, stereopsis, convergence, complete ocular motility, strabismus examination, slit-lamp anterior segment examination and fundus ophthalmoscopy were collected and reviewed.

Results

A total of 253 patients (203 ASD and 50 healthy controls) were included in the study. A higher number of total orthoptic defects were detected in the ASD group, in comparison with the control group. Specifically, a higher percentage of stereopsis deficit and convergence insufficiency was observed.

Conclusion

In our cohort of children with ASD stereopsis deficit, convergence insufficiency and refractive errors are the most observed ocular conditions. These findings are consistent with the known alterations of motor skills and sensory processing in ASD. Moreover, our study supports the hypothesis that visual acuity is not compromised in children with ASD. As a result, a complete ophthalmic evaluation is highly recommended in children with ASD, to guarantee early detection and treatment of possible visual-motor defects.
Patient safety discourse in a pandemic: a Twitter hashtag analysis study on #PatientSafety
Authors: Litvinova Olena , Matin Farhan Bin , Matin Maima , Zima-Kulisiewicz Bogumila , Tomasik Cyprian , Siddiquea Bodrun Naher , Stoyanov Jivko , Atanasov Atanas G. , Willschke Harald
Published: November 2023

Background

The digitalization of medicine is becoming a transformative force in modern healthcare systems. This study aims to investigate discussions regarding patient safety, as well as summarize perceived approaches to mitigating risks of adverse events expressed through the #PatientSafety Twitter hashtag during the COVID-19 pandemic.

Methods

This research is grounded in the analysis of data extracted from Twitter under the hashtag #PatientSafety between December 1, 2019 and February 1, 2023. Symplur Signals, which represents a tool offering a method to monitor tweets containing hashtags registered with the Symplur Healthcare Hashtag Project, was used for analyzing the tweets shared in the study period. For text analytics of the relevant data, we further used the word cloud generator MonkeyLearn, and VOSviewer.

Results

The analysis encompasses 358′809 tweets that were shared by 90′079 Twitter users, generating a total of 1′183’384′757 impressions. Physicians contributed to 18.65% of all tweets, followed by other healthcare professionals (14.31%), and health-focused individuals (10.91%). Geographically, more than a third of tweets (60.90%) were published in the United States. Canada and India followed in second and third positions, respectively. Blocks of trending terms of greater interest to the global Twitter community within the hashtag #PatientSafety were determined to be: “Patient,” “Practical doctors,” and “Health Care Safety Management.” The findings demonstrate the engagement of the Twitter community with COVID-19 and problems related to the training, experience of doctors and patients during a pandemic, communication, the vaccine safety and effectiveness, and potential use of off-label drugs. Noteworthy, in the field of pharmacovigilance, Twitter has the possibility of identifying adverse reactions associated with the use of drugs, including vaccines. The issue of medical errors has been also discussed by Twitter users using the hashtag #PatientSafety.

Conclusion

It is clear that various stakeholders, including students, medical practitioners, health organizations, pharmaceutical companies, and regulatory bodies, leverage Twitter to rapidly exchange medical information, data on the disease symptoms, and the drug effects. Consequently, there is a need to further integrate Twitter-derived data into the operational routines of healthcare organizations.
Why Are Clouds Over the Southern Ocean Super-Cool?
Authors: Fiddes Sonya L. , Mallet Marc D. , Alexander Simon P. , Protat Alain
Published: November 2023

When water drops below 0° Celsius, we assume it turns to ice. This is not always true though, especially inside of some clouds over the Southern Ocean. When water exists as a liquid below 0°C, it is known as super-cooled liquid water. Water only stays liquid below 0°C if it is extremely pure. Pollution and dust are two examples of impurities that can help water freeze. The water in Southern Ocean clouds has had very little contact with pollution or dust, so these clouds are often made up of super-cooled liquid water instead of ice. Super-cooled liquid water clouds reflect more sunlight back out into space than ice clouds do. Whether clouds are made of liquid or ice can control how warm the ocean is. This makes Southern Ocean clouds super cool as well as super important for the Earth’s climate!
“There just isn't any other option—so we just have to put up with it”: mental health in women's cycling and the necessity of structural change
Authors: Colangelo Jill , Smith Alexander , Buadze Anna , Liebrenz Michael
Published: November 2023

Historically, bicycle riding connoted freedom, independence, and enhanced mental and physical wellbeing for women. Persevering through criticism and moral panic, female cyclists have been competitive since the late 19th century—many earning substantial prize money and prestige. Unfortunately, this progress was not linear in its trajectory and contemporary professional women's cycling continues to be pervaded by structural and cultural challenges, which can have deleterious effects on athlete mental health. Notably, socioeconomic pressures endure, like unstable employment terms, limited team support, and role conflicts. Furthermore, sexual harassment, body shaming, and manipulation may characterize women's experiences with their coaches and teams. Sizable investment gaps between men's and women's teams and competitions often underpin these scenarios of disadvantage. Alongside hindering the development of women's cycling, these adverse circumstances may induce psychosocial risk factors. Within this context, by highlighting sport-specific and sex-specific considerations, the emerging subdiscipline of sports psychiatry can be valuable for protecting and promoting athlete welfare in women's cycling. Raising awareness about extant symptoms, vulnerabilities, contributing behaviours, and systemic issues, can bolster efforts to develop better conditions and care equivalence. To that end, this perspective article draws upon anecdotal and scholarly evidence to provide an overview of psychiatric concerns in women's professional cycling. This informs recommended strategies to improve mental health and advance equality within the sport, which should involve actions from several stakeholders, such as athletes, teams, and governing bodies.
A systematic review and meta-regression analysis of technical efficiency in Ethiopian wheat farming
Authors: Mulusew Asmamaw , Hong Mingyong
Published: November 2023

Replications are important for science, both statistically and philosophically. A plethora of empirical studies that have been done so far show that there are always variations, errors and inconclusive findings on the technical efficiency (TE) scores of wheat output in Ethiopia. In this study, we intend to contribute to these controversies by providing the first thorough investigation of the magnitude of mean-effect size and the underlying factors of mean technical efficiency variations in wheat production across studies in Ethiopia. These efficiency scores are retrieved from 31 studies, or a household size of 12,754 over the years 2011–2022. The analysis of meta-regression was done using a random effect model using Comprehensive Meta-analysis (CMA-4) software. The Begg and Mazumdar rank-correlation test, egger test, and funnel plot were used to determine publication bias across studies. Since 2022, we have utilized the classic fail-safe-N test. One issue with publication bias is the omission of some non-significant studies from the analysis, which, if they were included, would cancel out the observed effect. Further, the missing study problem or “file drawer” problem, of the study would also be computed using the “Trim and Fill” estimation approach. To evaluate heterogeneity, a forest plot is used. The effect size is also determined using the random effect model along with the I-squared statistic, the tau-squared and tau, and the Q-test. As a result, a mean effect size of 71.6% with a 95% confidence interval of 67.9%−75.3% is observed. The meta-regression demonstrates that the 31 mean technical efficiency studies included in the research had varying efficiency scores due to variations in sample size, methodologies used, publication status, and study range. It suggests that, with all other factors held constant, a 1% change in these regressors causes a 0.01%, 10.88%, 21.22%, and 6.74% change, respectively, in the mean technical efficiency scores across studies in Ethiopia. In addition, the technical efficiency score across studies is also affected by the location in which the study is conducted.
Effects of mid-to-late prepartum feed supplementation in Hanwoo beef cows on their performance, blood metabolites, and the carcass characteristics and metabolites of their neonatal calves
Authors: Park Myung Sun , Shokrollahi Borhan , Kim Ui Hyung , Won Jeong Il , Cho Soo-Hyun , Jin Shil , Kang Sung Sik , Moon Sung Jin , Um Kyung-Hwan , Jang Ki Suk , Kim Hyoun Ju , Kim Nam Young , Kim Sung Woo , Jang Sun Sik , Lee Hyun-Jeong
Published: November 2023

Introduction

This study aimed to evaluate the implications of supplementary nutrition during the mid-to-late pregnancy on various parameters in Hanwoo cows and their subsequent neonatal calves.

Materials and methods

Eight Hanwoo cows in their first parity were divided into two groups. The control group (C, 100%) received 3kg of concentrate and 5kg of rice straw throughout the pregnancy period, while the treatment group (T, 150%) increased their diet during mid-to-late pregnancy. Both performance assessments and blood metabolite analyses were performed for the pregnant cows. Neonatal calves were subjected to morphometric evaluations, blood sampling, and detailed morphometric analyses of carcasses and gastrointestinal components.

Results

Performance indices of the cows showed that both Pregnancy Period (PregP) and Body Condition Score (BCS) were significantly improved with supplemental feeding (p <0.05). Improvements in Body Weight (BW) and Feed Conversion Ratio (FCR) were not statistically significant. Blood metabolite analysis for the cows revealed decreased levels of triglycerides (TGLate), non-esterified fatty acids (NEFALate), and progesterone (P4Late), with a notable increase in glucose (GluLate) levels (p <0.01). In the neonatal calves, anatomical metrics of the gastrointestinal tissues showed increased Omasum Width (OmasWdth) values in the supplemented group (p =0.053). There was significant increase of papillae and villus lengths in the rumen and small intestine (p <0.01 and p <0.05, respectively). Morphometric evaluations displayed longer body lengths (BLnth) and larger chest width (ChestWdth) in the treated calves (p <0.05 and p <0.01, respectively). Carcass characteristics showed no substantial variations between the groups, while blood analysis in the calves revealed decreased GPT levels in the nutritionally supplemented group (p<0.05).

Discussion

The findings indicate that supplementing the diets of Hanwoo cows during mid-to-late pregnancy leads to significant changes in select maternal blood metabolites and influences specific anatomical and morphometric features in neonatal calves, all without significant shifts in carcass attributes.
Effects of different wheat bran fermentation sources on growth performance, nutrient digestibility, serum antioxidant capacity and fecal microbiota in growing pigs
Authors: Liu Heng , Ren Xiaojie , Li Yang , Cao Qingjie , Yang Lijie , Jiang Shuzhen , Fu Jiawei , Gao Jie , Yan Lei , Li Junxun , Yang Weiren
Published: November 2023

The present study aimed to evaluate the application of different wheat bran fermentation sources in growing pigs. A total of 320 pigs (43 ± 0.21 kg), were randomly allocated to 5 groups in a 21-d trial. The control group was fed a basal diet (CON) containing raw wheat bran, and the other four treatments were fed the diets in which the raw wheat bran in the basal diet was substituted with Aspergillus niger (WBA), Bacillus licheniformis (WBB), Candida utilis (WBC), and Lactobacillus plantarum (WBL) fermented wheat bran, respectively. The results showed that compared to the CON group, the crude fiber and pH values were decreased (p < 0.05), while the gross energy (GE), crude protein (CP), and lactic acid values were increased (p < 0.05) in all the wheat bran fermented by different strains. Compared with other treatments, feeding B. licheniformis fermented wheat bran had higher final weight, average daily gain, as well as lower feed-to-gain ratio. Compared with CON group, pigs fed with fermented wheat bran diets had higher dry matter, CP, and GE availability, serum total protein, albumin and superoxide dismutase levels, and fecal Lactobacillus counts, as well as lower malondialdehyde level and fecal Escherichia coli count. Collectively, our findings suggested that feeding fermented wheat bran, especially B. licheniformis fermented wheat bran, showed beneficial effects on the growth performance, nutrient digestibility, serum antioxidant capacity, and the gut microbiota structure of growing pigs.
Influence of Guar Meal from Pig Compound Feed on Productive Performance, Nitrogen Metabolism, and Greenhouse Gas Emissions
Authors: Mihaila Gabriel , Habeanu Mihaela , Lefter Nicoleta , Gheorghe Anca , Dumitru Mihaela , Marin Iuliana , Vidu Livia , Nicolae Carmen Georgeta , Popa Dana , Marin Monica
Published: November 2023

Guar (Cyamopsis tetragonoloba) is an annual legume tolerant to drought. Guar meal (GM) is a protein- and carbohydrate-rich co-product generated after the mechanical separation of the endosperm from the germ and hull of guar seed. GM has received considerable interest in animal feed as an alternative to soybean meal (SM). In this study, we aimed to assess the nitrogen (N) balance indicators, performance, carcass traits, and main greenhouse gas (GHG) emissions resulting from enteric fermentation (E-CH4) and manure (M-CH4 and N2O). Two tests were performed: (i) a biological trial on 45 pigs (15 animals/group) and (ii) a digestibility test in metabolism cages (N = 15, 5 replicates/group). Three different diets were given to the pigs: one diet was based on 0% GM (SM diet); in the second, GM-50%, GM replaced 50% of the SM; and the third was GM-100%, in which GM fully replaced the SM. The GM and SM diets were analyzed for their proximate composition. A model based on prediction equations was used to estimate the GHGs. GM up to 10% in the diets of finishing pigs did not significantly impact growth performance or carcass traits, although a slight increase in neutral detergent fiber (NDF) was observed. GM up to 10% improved N digestibility (p < 0.0001), net protein utilization (p < 0.0001), the biological value of protein, coefficients of metabolizability, and the coefficient of the total tract’s apparent digestibility. Irrespective of its dietary proportion, GM decreased total nitrogen output (TNO, p = 0.11). A highly significant impact was noted for N2O and E-CH4 (for DM, p < 0.0001), as well as a significant impact for E-CH4, expressed as g CO2 Eq (p = 0.007), and g CO2 Eq. LU (livestock unit, p = 0.005), also reported as ADG (p = 0.024). Manure, M-CH4, was not significantly influenced. In conclusion, GM can replace up to 100% SM and is thus a valuable byproduct that does not alter animal performance and can positively impact N2O and E-CH4.
Wastewater-Based Nutrient Supply for Lettuce Production in the Infulene Valley, Maputo, Mozambique
Authors: Niquice-Janeiro Celma Almerinda , Arsénio Andre Marques , van Lier Jules Bernardus
Published: November 2023

This research investigated the contribution of wastewater-based nutrient supply, viz., nitrogen (N), phosphorous (P), and potassium (K), for lettuce production in the Infulene Valley, Mozambique, from July to September 2019. The research was conducted in groundwater- and wastewater-irrigated agricultural plots. Water samples were collected weekly, soil samples were collected before planting and after harvest, and lettuce samples were collected at harvest time. The nutrient content (N, P, and K) was measured, and a mass balance method was applied. Wastewater had distinctly higher nutrient contents than groundwater, which guaranteed crop nutrition during the growing stage. Wastewater contributed 88%, 96%, and 97% to the N, P, and K requirements, respectively. The crop yield in the wastewater-irrigated areas was 43,8 ± 16 tons/ha, which was higher than 35 ± 8 tons/ha observed for the groundwater-irrigated areas, but results showed no statistically significant differences. Conclusively, wastewater led to reduced soil-nutrient gap and can be a source of nutrients. Therefore, wastewater is regarded as an alternative nutrient source of interest, and if properly applied, it might reduce environmental health hazards, resulting from run-off or leaching of excess nutrients.
A Comparison of Haematological and Biochemical Profiles between Intrauterine Growth Restriction and Normal Piglets at 72 Hours Postpartum
Authors: Ayala Lucía , Sánchez Cristian Jesús , Hernández Fuensanta , Madrid Josefa , López Miguel José , Martínez-Miró Silvia
Published: November 2023

Intrauterine growth restriction in piglets has been a problem in the pig industry due to genetic selection based on hyperprolificacy. This has led to an increase in the number of underweight piglets and a worsening of the survival rate. The goal of this study was to enhance the knowledge of differences between normal and IUGR piglets a few hours after birth in terms of haematological variables, biochemical parameters, and immunoglobulin levels. Two groups of 20 piglets each were assessed. The control group (N) was made up of piglets with weights greater than 1500 g, and the IUGR group consisted of piglets weighing 500–1000 g and with at least two IUGR features. Blood samples were collected 72 h after birth for analysis of the red and white blood cell parameters, reticulocyte indices, platelet indices, biochemical parameters, and immunoglobulin levels. Alterations in red blood cells and reticulocytes, a lower lymphocyte count, hyperinsulinemia, and high oxidative stress were observed in IUGR piglets (p < 0.05). In contrast, differences were not observed (p > 0.05) in the serum immunoglobulin level. It can be concluded that the haematological and biochemical differences in IUGR piglets with respect to normal-weight piglets are present at birth indicating possible alterations in immunity, metabolism, and redox status; therefore, IUGR piglets could be more vulnerable to illness and future disorders, such as metabolic syndrome.
The Oral Microbiome across Oral Sites in Cats with Chronic Gingivostomatitis, Periodontal Disease, and Tooth Resorption Compared with Healthy Cats
Authors: Anderson Jamie G. , Rojas Connie A. , Scarsella Elisa , Entrolezo Zhandra , Jospin Guillaume , Hoffman Sharon L. , Force Judy , MacLellan Roxane H. , Peak Mike , Shope Bonnie H. , Tsugawa Anson J. , Ganz Holly H.
Published: November 2023

Feline chronic gingivostomatitis (FCGS) is a chronic mucosal and gingival inflammatory disease in which pathogenesis remains unclear. Interactions between the host inflammatory process, the host immune response, and the oral microbiome are implicated in this pathogenesis. To begin to understand this disease and the impact of the microbiome to host inflammatory disease states, we collected sterile noninvasive plaque biofilm samples from ten distinct sites within the oral cavity in cats with stomatitis (n = 12), healthy cats (n = 9), and cats with tooth resorption or periodontitis (n = 11). Analysis of full-length 16S rRNA gene sequences indicated that the microbiomes of cats with FCGS presented marked dysbiosis at multiple oral sites. Additionally, microbiome beta diversity varied with oral condition, indicating that stomatitis, periodontitis, and/or tooth resorption influence the microbiome differently. Lastly, we found that the microbiomes of swabs taken from the oral cavity were comparable to those taken from plaque using endodontic paper points, validating this as another sampling method. Collectively, our work furthers our understanding of the dysbiosis and composition of bacteria in the oral microbiome in FCGS, with hopes of contributing to the prevention, diagnosis, and treatment of this challenging condition in felines.
Drug Discovery
Authors: Nikolova Stoyanka
Published: November 2023

More than 50% of deaths worldwide are linked to chronic inflammatory disorders, including cancer, cardiovascular disease, dementia, stroke, and diabetes [...]
A Blockchain-Based Privacy-Preserving and Fair Data Transaction Model in IoT
Authors: Zhou Wei , Zhang De , Han Guangjie , Zhu Wenyin , Wang Xupeng
Published: November 2023

The rapid development of the Internet of Things (IoT) has resulted in vast amounts of widely distributed data. Sharing these data can spur innovative advancements and enhance service quality. However, conventional data-sharing methods often involve third-party intermediaries, posing risks of single-point failures and privacy leaks. Moreover, these traditional sharing methods lack a secure transaction model to compensate for data sharing, which makes ensuring fair payment between data consumers and providers challenging. Blockchain, as a decentralized, secure, and trustworthy distributed ledger, offers a novel solution for data sharing. Nevertheless, since all nodes on the blockchain can access on-chain data, data privacy is inadequately protected, and traditional privacy-preserving methods like anonymization and generalization are ineffective against attackers with background knowledge. To address these issues, this paper proposes a decentralized, privacy-preserving, and fair data transaction model based on blockchain technology. We designed an adaptive local differential privacy algorithm, MDLDP, to protect the privacy of transaction data. Concurrently, verifiable encrypted signatures are employed to address the issue of fair payment during the data transaction process. This model proposes a committee structure to replace the individual arbitrator commonly seen in traditional verifiable encrypted signatures, thereby reducing potential collusion between dishonest traders and the arbitrator. The arbitration committee leverages threshold signature techniques to manage arbitration private keys. A full arbitration private key can only be collaboratively constructed by any arbitrary t members, ensuring the key’s security. Theoretical analyses and experimental results reveal that, in comparison to existing approaches, our model delivers enhanced transactional security. Moreover, while guaranteeing data availability, MDLDP affords elevated privacy protection.
Free Vibration Analysis of Arches with Interval-Uncertain Parameters
Authors: Nie Zhihua , Ren Xingmin , Yang Yongfeng , Fu Chao , Zhao Jiepeng
Published: November 2023

The dynamic characteristics of a structure serve as a crucial foundation for structural assessment, fault diagnosis, and ensuring structural safety. Therefore, it is imperative to investigate the impacts of uncertain parameters on the dynamic performance of structures. The dynamic characteristics of arches with uncertain parameters are analyzed in this paper. The uncertain parameters are regarded as non-probabilistic uncertainties and represented as interval variables. A model of an arch considering interval-uncertain parameters is built, and kinematic equations are established. The natural frequencies are obtained using the differential quadrature (DQ) method, and the relationships between natural frequency, radius, and central angle are also analyzed. On this basis, the Chebyshev polynomial surrogate (CPS) model is employed to solve the uncertain dynamic problem, and the natural frequencies are seen to be the objective functions of the CPS model. The accuracy verification of the model is achieved by comparison with the Monte Carlo simulation (MCS). Simulations are carried out considering different uncertainties, and the results show that the bounds of natural frequencies are influenced not only by the types of uncertain parameters, but also by their combinations.
Evaluation of the Efficacy of the VHO-Osthold® Perfect Orthonyxic Buckle While Treating Ingrown Nails and Its Effect on the Clinical Picture of the Nail Apparatus
Authors: Kuros Faustyna , Wcisło-Dziadecka Dominika
Published: November 2023

Background: An ingrown nail (IN—ingrowing nail) is a very troublesome condition that mainly affects the big toe in all age groups and involves the ingrowth of part of the nail plate into the soft periungual tissues, causing an inflammatory reaction, pain, and even a bacterial infection. As a result of the evolution of inflammation, highly vascularized and painful granulation tissue is formed, visible in the side walls, which causes difficulties in walking, while reducing the quality of life of patients in everyday functioning. The aim of the study was to evaluate the effectiveness of the VHO-Osthold® Perfect orthonyxic buckle while treating ingrown nails and its effect on the clinical picture of the nail apparatus. Methods: A prospective observational study was conducted on 39 patients with ingrown toenails in Scholz stage 3a–5b who underwent therapy with the use of a VHO-Osthold® Perfect orthonyxic brace. An original questionnaire form was used to assess the therapy, consisting of several parts concerning the type and stage of the ingrown nails, the location and aetiopathogenesis of the ingrown nails, pain, and the patients’ life quality before and after therapy. Results: All elements improved significantly after treatment (p < 0.05 each). Twelve months after the end of therapy, none of the probands reported disease relapse. The predominant location of ingrown nails was both big toes (69.2% for each foot). The most common group of reasons was outer pressure—all patients experienced at least one issue, with frequent mechanical injuries being the most common (59.0%), followed by wearing too-small shoes (46.2%). Almost half of the patients declared no worries during treatment (48.7%). Life quality assessment grew from 1.82 ± 1.02 to 6.41 ± 0.72 on a scale from 1 to 7, p < 0.001. Conclusions: The VHO-Osthold® Perfect clamp eliminates pain after application to the ingrown toenail and allows for a safe and conservative approach to the treatment of ingrown toenails, improving the clinical picture of the nail apparatus, general well-being, and the quality of life of patients with advanced ingrown toenails.
Statistical Homogenization of Elastic and Fracture Properties of a Sample Selective Laser Melting Material
Authors: Connor Ryan P. , Vemparala Balavignesh , Abedi Reza , Huynh Giang , Soghrati Soheil , Feldmeier Chris T. , Lamb Kevin
Published: November 2023

Selective laser melting (SLM) is an additive manufacturing technique commonly used in the rapid prototyping of components. The complexity of the SLM microstructure poses a unique challenge to deriving effective mechanical properties at different length scales. Representative volume elements (RVEs) are often used to homogenize the material properties of composites. Instead of RVEs, we use statistical volume elements (SVEs) to homogenize the elastic and fracture properties of the material. This relates the inherent variation of a material’s microstructure to the variation in its mechanical properties at different observation scales. The convergence to the RVE limit is examined from two perspectives: the stability of the mean value as the SVE size increases for the mean-based approach, and the tendency of the normalized variation in homogenized properties to zero as the SVE size increases for the variation-based approach. Fracture properties tend to make the RVE limit slower than do elastic properties from both perspectives. There are also differences between vertical (normal to printing plane) and horizontal (in-plane) properties. While the elastic properties tend to make the RVE limit faster for the horizontal direction, i.e., having a smaller variation and more stable mean value, the fracture properties exhibit the opposite effect. We attributed these differences to the geometry of the melt pools.
Analysis of the Influence of PET and Glass Packaging Waste Materials on the Physical and Mechanical Properties of Cementitious Composites
Authors: Zapała-Sławeta Justyna , Mazurek Grzegorz , Woś Jakub
Published: November 2023

The limited availability of natural resources, such as sand, and the need to reduce CO2 emissions, which are produced in large quantities in the production of binding materials, indicate the need to look for alternative raw materials used in construction materials. At the same time, there is a strong need to utilise waste packaging materials, the global production of which is constantly increasing. This work aims to investigate the possibility of using recycled polyethylene terephthalate (PET), utilised as a partial substitute for fine aggregate, and waste glass, implemented as powder, serving as a partial substitute for cement in the manufacturing of the cementitious composites. An experimental study was carried out to evaluate the physical and mechanical properties of the resultant cementitious composites. The incorporated PET aggregate comprised 0%, 5%, and 10% by volume of silica sand and 0%, 10%, and 20% glass powder by weight of cement. The addition of waste raw materials augmented the flow of fresh mortars, predominantly subsequent to the introduction of PET recyclate. The deployment of artificial aggregate in mortars induces a decrease in the volumetric density. Concurrently, the mechanical properties of mortars enriched with waste materials exhibited a reduction, in terms of both compressive and flexural strength, with the detriment escalating in conjunction with the content of waste raw materials. An analysis of statistical significance of effects, grounded in an analysis of variance, is delineated within this document, pinpointing the quantities of waste raw materials that can be assimilated in mortars without inducing a substantial deterioration of strength properties. Through studies on phase composition, it has been demonstrated that the utilised glass waste, possessing a grain size analogous to cement, exhibited poor pozzolanic properties. The test results indicate that it is possible to partially replace cement with glass powder, up to 10%, and fine aggregate with PET waste, up to 5%, without a significant reduction in the mechanical properties of the material.
Nanobiotechnology in Bone Tissue Engineering Applications: Recent Advances and Future Perspectives
Authors: Iqbal Neelam , Pant Tejal , Rohra Nanda , Goyal Abhishek , Lawrence Merin , Dey Anomitra , Ganguly Payal
Published: November 2023

Bone regeneration and repair are complex processes with the potential of added complications, like delayed repair, fracture non-union, and post-surgical infections. These conditions remain a challenge globally, pressurizing the economy and patients suffering from these conditions. Applications of nanotechnology (NBT) in the field of medicine have provided a medium for several approaches to support these global challenges. Tissue engineering is one such field that has been on the rise in the last three decades through the utilization of NBT for addressing the challenges related to bone regeneration. First, NBT enables the formation of scaffolds at the nanoscale needed for bone tissue engineering (BTE) using natural and synthetic polymers, as well as with minerals and metals. Then, it aids the development of the nano-formulation strategized to deliver antimicrobial drugs and/or growth factors through various ways to enhance bone repair through the scaffold. Third, NBT facilitates the use of specialized nanoparticles to image and track cellular events in vitro as well as in vivo. This review is an effort to bring together the current knowledge in the field of BTE and present the scope of ever-evolving NBT, a contribution towards precision medicine.
Hydroxytyrosol Counteracts Triple Negative Breast Cancer Cell Dissemination via Its Copper Complexing Properties
Authors: Perta Nunzio , Torrieri Di Tullio Laura , Cugini Elisa , Fattibene Paola , Rapanotti Maria Cristina , Borromeo Ilaria , Forni Cinzia , Malaspina Patrizia , Cacciamani Tiziana , Di Marino Daniele , Rossi Luisa , De Luca Anastasia
Published: November 2023

Polyphenols have gained increasing attention for their therapeutic potential, particularly in conditions like cancer, due to their established antioxidant and anti-inflammatory properties. Recent research highlights their ability to bind to transition metals, such as copper. This is particularly noteworthy given the key role of copper both in the initiation and progression of cancer. Copper can modulate the activity of kinases required for the epithelial–mesenchymal transition (EMT), a process fundamental to tumor cell dissemination. We have previously demonstrated the copper-binding capacity of oleuropein, a secoiridoid found in Olea europaea. In the present study, we investigated the effect of hydroxytyrosol, the primary oleuropein metabolite, on the metastatic potential of three triple-negative breast cancer cell lines (MDA-MB-231, MDA-MB-468, and SUM159). We found that hydroxytyrosol modulated the intracellular copper levels, influencing both the epithelial and mesenchymal markers, by downregulating copper-dependent AKT phosphorylation, a member of the EMT signaling cascade, through Western blot, RT-qPCR, and immunofluorescence. Indeed, by optical spectra, EPR, and in silico approaches, we found that hydroxytyrosol formed a complex with copper, acting as a chelating agent, thus regulating its homeostasis and affecting the copper-dependent signaling cascades. While our results bring to light the copper-chelating properties of hydroxytyrosol capable of countering tumor progression, they also provide further confirmation of the key role of copper in promoting the aggressiveness of triple-negative breast cancer cells.
Prostaglandin E2 Boosts the Hyaluronan-Mediated Increase in Inflammatory Response to Lipopolysaccharide by Enhancing Lyve1 Expression
Authors: Hog Pauline , Kuntschar Silvia , Rappl Peter , Huard Arnaud , Weigert Andreas , Brüne Bernhard , Schmid Tobias
Published: November 2023

Macrophages are a highly versatile and heterogenic group of immune cells, known for their involvement in inflammatory reactions. However, our knowledge about distinct subpopulations of macrophages and their specific contribution to the resolution of inflammation remains incomplete. We have previously shown, in an in vivo peritonitis model, that inhibition of the synthesis of the pro-inflammatory lipid mediator prostaglandin E2 (PGE2) attenuates efficient resolution of inflammation. PGE2 levels during later stages of the inflammatory process further correlate with expression of the hyaluronan (HA) receptor Lyve1 in peritoneal macrophages. In the present study, we therefore aimed to understand if PGE2 might contribute to the regulation of Lyve1 and how this might impact inflammatory responses. In line with our in vivo findings, PGE2 synergized with dexamethasone to enhance Lyve1 expression in bone marrow-derived macrophages, while expression of the predominant hyaluronan receptor CD44 remained unaltered. PGE2-mediated Lyve1 upregulation was strictly dependent on PGE2 receptor EP2 signaling. While PGE2/dexamethasone-treated macrophages, despite their enhanced Lyve1 expression, did not show inflammatory responses upon stimulation with low (LMW) or high-molecular-weight hyaluronan (HMW)-HA, they were sensitized towards LMW-HA-dependent augmentation of lipopolysaccharide (LPS)-induced inflammatory responses. Thus, Lyve1-expressing macrophages emerged as a subpopulation of macrophages integrating inflammatory stimuli with extracellular matrix-derived signals.
Paraventricular Mast Cell-Derived Histamine Activates CRH Neurons to Mediate Adult Visceral Hypersensitivity Induced by Neonatal Maternal Separation
Authors: Chen Ziyang , Zhou Tiantian , Li Yunfan , Li Tingting , Ding Zhengnian , Liu Li
Published: November 2023

Neonatal maternal separation (NMS) is an early-life stress (ELS) that can result in adult visceral hypersensitivity, which is usually manifested as chronic visceral pain. Although mast cells and corticotropin-releasing hormone (CRH) neurons are involved in stress response, whether there is an interaction between mast cells and CRH neurons in hypothalamic paraventricular nucleus (PVN) during the ELS-induced visceral hypersensitivity remains elusive. Herein, we established an NMS model by separating neonatal mice from their mothers, and observed that these mice presented visceral hypersensitivity in adulthood, as indicated by elevated abdominal withdrawal reflex and lowered visceral pain threshold. The NMS-induced adult visceral hypersensitivity was accompanied by activation of mast cells and CRH neurons in PVN. Also, NMS increased the histamine content (an inflammatory mediator mainly released by mast cells) and histamine H2 receptor (H2R) expression of CRH neurons in PVN. Remarkably, intra-PVN administration with mast cell stabilizer attenuated the NMS-induced CRH neuronal activation and adult visceral pain, while histamine administration showed the opposite effects. Moreover, intra-PVN injection with H2R antagonist alleviated the NMS-induced CRH neuronal activation, PKA and CREB phosphorylation, and importantly, adult visceral pain. Together, our findings revealed a role of an interaction between paraventricular mast cells and CRH neurons in NMS-induced adult visceral hypersensitivity, thereby providing a perspective for the management of visceral pain.
Impact of Teaching Palliative Psychology with Psychodrama and Arts Therapies on Psychology Students in Klagenfurt
Authors: Wieser Michael Alexander , Leitner Alexandra
Published: November 2023

The Erasmus+ project “Death Education for Palliative Psychology” (DE4PP) under the coordination of the University of Padua investigated the effects of teaching palliative psychology with psychodrama and arts therapies, as positive effects on students’ attitudes towards life and death were suspected through the use of these forms of treatment. Five countries participated in this project funded by the European Commission (Austria, Israel, Italy, Poland, and Romania). In Klagenfurt (Austria), 34 students from the University of Klagenfurt completed the pilot course entitled “Palliative Psychology” generated by the project partners. Course participants filled out psychological and satisfaction questionnaires at the beginning and end of the course, to measure the impact of teaching palliative psychology with psychodrama and arts therapies. The research involved a control group. In addition, six participants in the course participated in a focus group interview at the end, which included questions about their experience of the course; the impact of arts therapies and psychodrama techniques; experiences of verbal and artistic processing of death anxiety; and the meaning of life and death, representation of death, and associated feelings. The results, in summary, showed that processing of life and death had occurred in the participants due to the course they had completed. As part of the course, creative arts and psychodrama were bridges to death. Filling out satisfaction questionnaires, photovoice assignments, role reversal, and visualizing a personal social atom were seen by students in Klagenfurt as particularly effective methods for self-reflection.